LOCUS       DBKEKI010000001        97352 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.001, whole genome shotgun sequence.
ACCESSION   DBKEKI010000001 DBKEKI010000000
VERSION     DBKEKI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 97352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.001"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..122
                     /locus_tag="ACUQPX_000001"
     CDS             <1..122
                     /locus_tag="ACUQPX_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HHV9054822.1"
                     /translation="IISNEVAPFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            136..1416
                     /gene="gabT"
                     /locus_tag="ACUQPX_000002"
     CDS             136..1416
                     /gene="gabT"
                     /locus_tag="ACUQPX_000002"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HHV9054823.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAKNCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            1654..3084
                     /gene="gabP"
                     /locus_tag="ACUQPX_000003"
     CDS             1654..3084
                     /gene="gabP"
                     /locus_tag="ACUQPX_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HHV9054824.1"
                     /translation="MGQSSQPHELGGGLKSRHVTSRHVTSRHVTMLSIAGVIGASLFV
                     GSSVAIAEAGPAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGY
                     TIGWLYWWFWVLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGE
                     FEFWLALCKVIAILAFIFLGAVAISGIYPYAEVSGISRLWDSGGFMPNGFGAVLSAML
                     ITMFSFMGAEIVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPG
                     LKAVGSYRSVLELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAV
                     MGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQ
                     LRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIG
                     IICTVPIMARWKKLVLWQKTPVHNTR"
     gene            3105..3767
                     /gene="csiR"
                     /locus_tag="ACUQPX_000004"
     CDS             3105..3767
                     /gene="csiR"
                     /locus_tag="ACUQPX_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HHV9054825.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(3768..4217)
                     /gene="kbp"
                     /locus_tag="ACUQPX_000005"
     CDS             complement(3768..4217)
                     /gene="kbp"
                     /locus_tag="ACUQPX_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HHV9054826.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVLEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(4301..4459)
                     /locus_tag="ACUQPX_000006"
     CDS             complement(4301..4459)
                     /locus_tag="ACUQPX_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HHV9054827.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            4642..4941
                     /locus_tag="ACUQPX_000007"
     CDS             4642..4941
                     /locus_tag="ACUQPX_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HHV9054828.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            4951..5475
                     /gene="ygaP"
                     /locus_tag="ACUQPX_000008"
     CDS             4951..5475
                     /gene="ygaP"
                     /locus_tag="ACUQPX_000008"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HHV9054829.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(5522..5926)
                     /gene="stpA"
                     /locus_tag="ACUQPX_000009"
     CDS             complement(5522..5926)
                     /gene="stpA"
                     /locus_tag="ACUQPX_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HHV9054830.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            6614..7063
                     /gene="alaE"
                     /locus_tag="ACUQPX_000010"
     CDS             6614..7063
                     /gene="alaE"
                     /locus_tag="ACUQPX_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HHV9054831.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(7100..7444)
                     /locus_tag="ACUQPX_000011"
     CDS             complement(7100..7444)
                     /locus_tag="ACUQPX_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HHV9054832.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            7596..7925
                     /locus_tag="ACUQPX_000012"
     CDS             7596..7925
                     /locus_tag="ACUQPX_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HHV9054833.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            8173..8418
                     /gene="nrdH"
                     /locus_tag="ACUQPX_000013"
     CDS             8173..8418
                     /gene="nrdH"
                     /locus_tag="ACUQPX_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HHV9054834.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPQAASA"
     gene            8415..8825
                     /gene="nrdI"
                     /locus_tag="ACUQPX_000014"
     CDS             8415..8825
                     /gene="nrdI"
                     /locus_tag="ACUQPX_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HHV9054835.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            8798..10942
                     /gene="nrdE"
                     /locus_tag="ACUQPX_000015"
     CDS             8798..10942
                     /gene="nrdE"
                     /locus_tag="ACUQPX_000015"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HHV9054836.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            10952..11911
                     /gene="nrdF"
                     /locus_tag="ACUQPX_000016"
     CDS             10952..11911
                     /gene="nrdF"
                     /locus_tag="ACUQPX_000016"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HHV9054837.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            12266..13468
                     /gene="proV"
                     /locus_tag="ACUQPX_000017"
     CDS             12266..13468
                     /gene="proV"
                     /locus_tag="ACUQPX_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HHV9054838.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLHAL
                     DREGVNNG"
     gene            13461..14525
                     /gene="proW"
                     /locus_tag="ACUQPX_000018"
     CDS             13461..14525
                     /gene="proW"
                     /locus_tag="ACUQPX_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HHV9054839.1"
                     /translation="MADKNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            14583..15575
                     /gene="proX"
                     /locus_tag="ACUQPX_000019"
     CDS             14583..15575
                     /gene="proX"
                     /locus_tag="ACUQPX_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HHV9054840.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            15767..16951
                     /locus_tag="ACUQPX_000020"
     CDS             15767..16951
                     /locus_tag="ACUQPX_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9054841.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCMTFANFSILWTSMAFLLAAPPFNYSDGDIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGATGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            17075..17812
                     /gene="ygaZ"
                     /locus_tag="ACUQPX_000021"
     CDS             17075..17812
                     /gene="ygaZ"
                     /locus_tag="ACUQPX_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HHV9054842.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            17802..18137
                     /gene="ygaH"
                     /locus_tag="ACUQPX_000022"
     CDS             17802..18137
                     /gene="ygaH"
                     /locus_tag="ACUQPX_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HHV9054843.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            18228..18758
                     /gene="emrR"
                     /locus_tag="ACUQPX_000023"
     CDS             18228..18758
                     /gene="emrR"
                     /locus_tag="ACUQPX_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HHV9054844.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            18885..20057
                     /gene="emrA"
                     /locus_tag="ACUQPX_000024"
                     /pseudo
     CDS             18885..20057
                     /gene="emrA"
                     /locus_tag="ACUQPX_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            20074..21612
                     /gene="emrB"
                     /locus_tag="ACUQPX_000025"
     CDS             20074..21612
                     /gene="emrB"
                     /locus_tag="ACUQPX_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HHV9054845.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRLAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(21676..22191)
                     /gene="luxS"
                     /locus_tag="ACUQPX_000026"
     CDS             complement(21676..22191)
                     /gene="luxS"
                     /locus_tag="ACUQPX_000026"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HHV9054846.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(22341..23897)
                     /gene="gshA"
                     /locus_tag="ACUQPX_000027"
     CDS             complement(22341..23897)
                     /gene="gshA"
                     /locus_tag="ACUQPX_000027"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HHV9054847.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGELPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(23970..24398)
                     /locus_tag="ACUQPX_000028"
     CDS             complement(23970..24398)
                     /locus_tag="ACUQPX_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HHV9054848.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(24395..24961)
                     /gene="yqaB"
                     /locus_tag="ACUQPX_000029"
     CDS             complement(24395..24961)
                     /gene="yqaB"
                     /locus_tag="ACUQPX_000029"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HHV9054849.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(25109..25185)
                     /locus_tag="ACUQPX_000030"
     tRNA            complement(25109..25185)
                     /locus_tag="ACUQPX_000030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25149..25151),aa:Arg,seq:acg)
     gene            complement(25248..25324)
                     /locus_tag="ACUQPX_000031"
     tRNA            complement(25248..25324)
                     /locus_tag="ACUQPX_000031"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25288..25290),aa:Arg,seq:acg)
     gene            complement(25389..25465)
                     /locus_tag="ACUQPX_000032"
     tRNA            complement(25389..25465)
                     /locus_tag="ACUQPX_000032"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25429..25431),aa:Arg,seq:acg)
     gene            complement(25469..25561)
                     /locus_tag="ACUQPX_000033"
     tRNA            complement(25469..25561)
                     /locus_tag="ACUQPX_000033"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25525..25527),aa:Ser,seq:gct)
     gene            complement(25877..26062)
                     /gene="csrA"
                     /locus_tag="ACUQPX_000034"
     CDS             complement(25877..26062)
                     /gene="csrA"
                     /locus_tag="ACUQPX_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HHV9054850.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(26297..28927)
                     /gene="alaS"
                     /locus_tag="ACUQPX_000035"
     CDS             complement(26297..28927)
                     /gene="alaS"
                     /locus_tag="ACUQPX_000035"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HHV9054851.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKQEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLGRTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVTEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(29055..29555)
                     /gene="recX"
                     /locus_tag="ACUQPX_000036"
     CDS             complement(29055..29555)
                     /gene="recX"
                     /locus_tag="ACUQPX_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HHV9054852.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(29798..30859)
                     /gene="recA"
                     /locus_tag="ACUQPX_000037"
     CDS             complement(29798..30859)
                     /gene="recA"
                     /locus_tag="ACUQPX_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HHV9054853.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(30939..31436)
                     /gene="pncC"
                     /locus_tag="ACUQPX_000038"
     CDS             complement(30939..31436)
                     /gene="pncC"
                     /locus_tag="ACUQPX_000038"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HHV9054854.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(31581..32666)
                     /gene="mltB"
                     /locus_tag="ACUQPX_000039"
     CDS             complement(31581..32666)
                     /gene="mltB"
                     /locus_tag="ACUQPX_000039"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HHV9054855.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            32922..33485
                     /gene="srlA"
                     /locus_tag="ACUQPX_000040"
     CDS             32922..33485
                     /gene="srlA"
                     /locus_tag="ACUQPX_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015795 - sorbitol transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HHV9054856.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            33482..34441
                     /gene="srlE"
                     /locus_tag="ACUQPX_000041"
     CDS             33482..34441
                     /gene="srlE"
                     /locus_tag="ACUQPX_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015795 - sorbitol transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HHV9054857.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVVVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            34452..34823
                     /gene="srlB"
                     /locus_tag="ACUQPX_000042"
                     /gene_synonym="gutB"
     CDS             34452..34823
                     /gene="srlB"
                     /locus_tag="ACUQPX_000042"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HHV9054858.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAENNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            34827..35606
                     /gene="srlD"
                     /locus_tag="ACUQPX_000043"
     CDS             34827..35606
                     /gene="srlD"
                     /locus_tag="ACUQPX_000043"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HHV9054859.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            35759..36070
                     /gene="gutM"
                     /locus_tag="ACUQPX_000044"
     CDS             35759..36070
                     /gene="gutM"
                     /locus_tag="ACUQPX_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HHV9054860.1"
                     /translation="MALGGWQISRFNRAFDTLCQQGRVGVGRSSGRFKPRVVVAIALD
                     DQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPHDPLSQNALSLALKLKR
                     G"
     gene            36137..36910
                     /gene="srlR"
                     /locus_tag="ACUQPX_000045"
                     /gene_synonym="gutR"
     CDS             36137..36910
                     /gene="srlR"
                     /locus_tag="ACUQPX_000045"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HHV9054861.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            36903..37868
                     /gene="gutQ"
                     /locus_tag="ACUQPX_000046"
     CDS             36903..37868
                     /gene="gutQ"
                     /locus_tag="ACUQPX_000046"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HHV9054862.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(37865..39379)
                     /gene="norR"
                     /locus_tag="ACUQPX_000047"
     CDS             complement(37865..39379)
                     /gene="norR"
                     /locus_tag="ACUQPX_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HHV9054863.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            39566..41005
                     /gene="norV"
                     /locus_tag="ACUQPX_000048"
     CDS             39566..41005
                     /gene="norV"
                     /locus_tag="ACUQPX_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HHV9054864.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            41002..42135
                     /gene="norW"
                     /locus_tag="ACUQPX_000049"
     CDS             41002..42135
                     /gene="norW"
                     /locus_tag="ACUQPX_000049"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HHV9054865.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRTDDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(42263..44515)
                     /gene="hypF"
                     /locus_tag="ACUQPX_000050"
     CDS             complement(42263..44515)
                     /gene="hypF"
                     /locus_tag="ACUQPX_000050"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HHV9054866.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLYQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWLAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(44668..45195)
                     /gene="hydN"
                     /locus_tag="ACUQPX_000051"
     CDS             complement(44668..45195)
                     /gene="hydN"
                     /locus_tag="ACUQPX_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HHV9054867.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(45344..46354)
                     /gene="ascG"
                     /locus_tag="ACUQPX_000052"
     CDS             complement(45344..46354)
                     /gene="ascG"
                     /locus_tag="ACUQPX_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HHV9054868.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            46614..48071
                     /gene="ascF"
                     /locus_tag="ACUQPX_000053"
     CDS             46614..48071
                     /gene="ascF"
                     /locus_tag="ACUQPX_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HHV9054869.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            48080..49504
                     /gene="ascB"
                     /locus_tag="ACUQPX_000054"
     CDS             48080..49504
                     /gene="ascB"
                     /locus_tag="ACUQPX_000054"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HHV9054870.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(49619..50038)
                     /locus_tag="ACUQPX_000055"
     CDS             complement(49619..50038)
                     /locus_tag="ACUQPX_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9054871.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(50035..50346)
                     /locus_tag="ACUQPX_000056"
     CDS             complement(50035..50346)
                     /locus_tag="ACUQPX_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HHV9054872.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(50508..51270)
                     /locus_tag="ACUQPX_000057"
                     /pseudo
     CDS             complement(50508..51270)
                     /locus_tag="ACUQPX_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(51313..51783)
                     /gene="hycI"
                     /locus_tag="ACUQPX_000058"
     CDS             complement(51313..51783)
                     /gene="hycI"
                     /locus_tag="ACUQPX_000058"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HHV9054873.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(51776..52186)
                     /gene="hycH"
                     /locus_tag="ACUQPX_000059"
     CDS             complement(51776..52186)
                     /gene="hycH"
                     /locus_tag="ACUQPX_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HHV9054874.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(52183..52950)
                     /gene="hycG"
                     /locus_tag="ACUQPX_000060"
     CDS             complement(52183..52950)
                     /gene="hycG"
                     /locus_tag="ACUQPX_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HHV9054875.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(52950..53492)
                     /gene="hycF"
                     /locus_tag="ACUQPX_000061"
     CDS             complement(52950..53492)
                     /gene="hycF"
                     /locus_tag="ACUQPX_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HHV9054876.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(53502..55211)
                     /gene="hycE"
                     /locus_tag="ACUQPX_000062"
     CDS             complement(53502..55211)
                     /gene="hycE"
                     /locus_tag="ACUQPX_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HHV9054877.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(55229..56152)
                     /gene="hycD"
                     /locus_tag="ACUQPX_000063"
     CDS             complement(55229..56152)
                     /gene="hycD"
                     /locus_tag="ACUQPX_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HHV9054878.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(56155..57981)
                     /gene="hycC"
                     /locus_tag="ACUQPX_000064"
     CDS             complement(56155..57981)
                     /gene="hycC"
                     /locus_tag="ACUQPX_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HHV9054879.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAIFKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(57978..58589)
                     /gene="hycB"
                     /locus_tag="ACUQPX_000065"
     CDS             complement(57978..58589)
                     /gene="hycB"
                     /locus_tag="ACUQPX_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HHV9054880.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(58714..59175)
                     /gene="hycA"
                     /locus_tag="ACUQPX_000066"
     CDS             complement(58714..59175)
                     /gene="hycA"
                     /locus_tag="ACUQPX_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HHV9054881.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            59387..59737
                     /gene="hypA"
                     /locus_tag="ACUQPX_000067"
     CDS             59387..59737
                     /gene="hypA"
                     /locus_tag="ACUQPX_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HHV9054882.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            59741..60613
                     /gene="hypB"
                     /locus_tag="ACUQPX_000068"
     CDS             59741..60613
                     /gene="hypB"
                     /locus_tag="ACUQPX_000068"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HHV9054883.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYRHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            60604..60876
                     /gene="hypC"
                     /locus_tag="ACUQPX_000069"
     CDS             60604..60876
                     /gene="hypC"
                     /locus_tag="ACUQPX_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HHV9054884.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            60876..61997
                     /gene="hypD"
                     /locus_tag="ACUQPX_000070"
     CDS             60876..61997
                     /gene="hypD"
                     /locus_tag="ACUQPX_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HHV9054885.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            61994..63004
                     /gene="hypE"
                     /locus_tag="ACUQPX_000071"
     CDS             61994..63004
                     /gene="hypE"
                     /locus_tag="ACUQPX_000071"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HHV9054886.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            63078..65146
                     /gene="flhA"
                     /locus_tag="ACUQPX_000072"
                     /pseudo
     CDS             63078..65146
                     /gene="flhA"
                     /locus_tag="ACUQPX_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(65183..65536)
                     /locus_tag="ACUQPX_000073"
     CDS             complement(65183..65536)
                     /locus_tag="ACUQPX_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HHV9054887.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(65568..65747)
                     /locus_tag="ACUQPX_000074"
     CDS             complement(65568..65747)
                     /locus_tag="ACUQPX_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9054888.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            65823..68384
                     /gene="mutS"
                     /locus_tag="ACUQPX_000075"
     CDS             65823..68384
                     /gene="mutS"
                     /locus_tag="ACUQPX_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HHV9054889.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNGPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            68490..69146
                     /gene="pphB"
                     /locus_tag="ACUQPX_000076"
     CDS             68490..69146
                     /gene="pphB"
                     /locus_tag="ACUQPX_000076"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HHV9054890.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     WPVYRMQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(69197..69964)
                     /gene="ygbI"
                     /locus_tag="ACUQPX_000077"
     CDS             complement(69197..69964)
                     /gene="ygbI"
                     /locus_tag="ACUQPX_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HHV9054891.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            70160..70932
                     /locus_tag="ACUQPX_000078"
                     /pseudo
     CDS             70160..70932
                     /locus_tag="ACUQPX_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            70929..72191
                     /gene="otnK"
                     /locus_tag="ACUQPX_000079"
     CDS             70929..72191
                     /gene="otnK"
                     /locus_tag="ACUQPX_000079"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HHV9054892.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMHHHPVNPMTDRYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            72188..72826
                     /locus_tag="ACUQPX_000080"
     CDS             72188..72826
                     /locus_tag="ACUQPX_000080"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HHV9054893.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            72831..73601
                     /locus_tag="ACUQPX_000081"
     CDS             72831..73601
                     /locus_tag="ACUQPX_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HHV9054894.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYNYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDA"
     gene            73696..75060
                     /locus_tag="ACUQPX_000082"
     CDS             73696..75060
                     /locus_tag="ACUQPX_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HHV9054895.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSCGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGVVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMVGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(75100..75336)
                     /locus_tag="ACUQPX_000083"
     CDS             complement(75100..75336)
                     /locus_tag="ACUQPX_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HHV9054896.1"
                     /translation="MICPRCADEQIEVMVKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(75347..76774)
                     /locus_tag="ACUQPX_000084"
     CDS             complement(75347..76774)
                     /locus_tag="ACUQPX_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HHV9054897.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTTMLAARK"
     gene            complement(76774..77367)
                     /locus_tag="ACUQPX_000085"
     CDS             complement(76774..77367)
                     /locus_tag="ACUQPX_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HHV9054898.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            77514..77921
                     /locus_tag="ACUQPX_000086"
     CDS             77514..77921
                     /locus_tag="ACUQPX_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HHV9054899.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(78041..79033)
                     /gene="rpoS"
                     /locus_tag="ACUQPX_000087"
     CDS             complement(78041..79033)
                     /gene="rpoS"
                     /locus_tag="ACUQPX_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HHV9054900.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSCMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(79096..80190)
                     /gene="nlpD"
                     /locus_tag="ACUQPX_000088"
     CDS             complement(79096..80190)
                     /gene="nlpD"
                     /locus_tag="ACUQPX_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HHV9054901.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(80375..81001)
                     /gene="pcm"
                     /locus_tag="ACUQPX_000089"
     CDS             complement(80375..81001)
                     /gene="pcm"
                     /locus_tag="ACUQPX_000089"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HHV9054902.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(80995..81756)
                     /gene="surE"
                     /locus_tag="ACUQPX_000090"
     CDS             complement(80995..81756)
                     /gene="surE"
                     /locus_tag="ACUQPX_000090"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HHV9054903.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(81737..82786)
                     /gene="truD"
                     /locus_tag="ACUQPX_000091"
     CDS             complement(81737..82786)
                     /gene="truD"
                     /locus_tag="ACUQPX_000091"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HHV9054904.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(82783..83262)
                     /gene="ispF"
                     /locus_tag="ACUQPX_000092"
     CDS             complement(82783..83262)
                     /gene="ispF"
                     /locus_tag="ACUQPX_000092"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HHV9054905.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTFGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(83262..83972)
                     /gene="ispD"
                     /locus_tag="ACUQPX_000093"
     CDS             complement(83262..83972)
                     /gene="ispD"
                     /locus_tag="ACUQPX_000093"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HHV9054906.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LMHDAARPCLHQDDLARLLALSENSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(83991..84302)
                     /gene="ftsB"
                     /locus_tag="ACUQPX_000094"
     CDS             complement(83991..84302)
                     /gene="ftsB"
                     /locus_tag="ACUQPX_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HHV9054907.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(84496..84819)
                     /locus_tag="ACUQPX_000095"
     CDS             complement(84496..84819)
                     /locus_tag="ACUQPX_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HHV9054908.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(84869..85474)
                     /gene="cysC"
                     /locus_tag="ACUQPX_000096"
     CDS             complement(84869..85474)
                     /gene="cysC"
                     /locus_tag="ACUQPX_000096"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HHV9054909.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(85474..86901)
                     /gene="cysN"
                     /locus_tag="ACUQPX_000097"
     CDS             complement(85474..86901)
                     /gene="cysN"
                     /locus_tag="ACUQPX_000097"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HHV9054910.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSLGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(86903..87811)
                     /gene="cysD"
                     /locus_tag="ACUQPX_000098"
     CDS             complement(86903..87811)
                     /gene="cysD"
                     /locus_tag="ACUQPX_000098"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HHV9054911.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            88064..89101
                     /gene="iap"
                     /locus_tag="ACUQPX_000099"
     CDS             88064..89101
                     /gene="iap"
                     /locus_tag="ACUQPX_000099"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HHV9054912.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KPPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(89235..90338)
                     /locus_tag="ACUQPX_000100"
                     /pseudo
     CDS             complement(89235..90338)
                     /locus_tag="ACUQPX_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(90532..90684)
                     /locus_tag="ACUQPX_000101"
     CDS             complement(90532..90684)
                     /locus_tag="ACUQPX_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HHV9054913.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(90948..91682)
                     /gene="cysH"
                     /locus_tag="ACUQPX_000102"
     CDS             complement(90948..91682)
                     /gene="cysH"
                     /locus_tag="ACUQPX_000102"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HHV9054914.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(91756..93468)
                     /gene="cysI"
                     /locus_tag="ACUQPX_000103"
     CDS             complement(91756..93468)
                     /gene="cysI"
                     /locus_tag="ACUQPX_000103"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HHV9054915.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILTSLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(93468..95267)
                     /gene="cysJ"
                     /locus_tag="ACUQPX_000104"
     CDS             complement(93468..95267)
                     /gene="cysJ"
                     /locus_tag="ACUQPX_000104"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HHV9054916.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            95586..95948
                     /gene="queD"
                     /locus_tag="ACUQPX_000105"
     CDS             95586..95948
                     /gene="queD"
                     /locus_tag="ACUQPX_000105"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HHV9054917.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            96026..97296
                     /locus_tag="ACUQPX_000106"
                     /pseudo
     CDS             96026..97296
                     /locus_tag="ACUQPX_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            97251..>97352
                     /locus_tag="ACUQPX_000107"
     CDS             97251..>97352
                     /locus_tag="ACUQPX_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HHV9054918.1"
                     /translation="MASGGRCYQESAMSVARNLWRVADAPHIVPADSV"
BASE COUNT        22956 a        26064 c        25058 g        23274 t
ORIGIN      
        1 gcattatttc caatgaagtg gccccattcg gcggcatcaa agcctcgggt ctgggtcgtg
       61 aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc atcggtcttt
      121 aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag tcaggcgatt
      181 ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcgaaaaa ctgccgggtg
      241 tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt gctcaatacc
      301 gggcacctgc atccgaaggt ggtggccgcg gtggaagcgc agttgaaaaa actgtcgcac
      361 acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat tatgaatcag
      421 aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc cgaagcggtg
      481 gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat cgcttttagc
      541 ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt gaatccgtac
      601 tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc ttgcccgctg
      661 cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa aaatgatgcc
      721 gcgccggaag atatcgccgc catcatgatt gagccggttc agggcgaagg cggtttctac
      781 gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca tgggatcatg
      841 ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt tgcgatggag
      901 cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg cggtttcccg
      961 ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg cggtctgggc
     1021 ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt gaaggtgttc
     1081 gagcaggaaa atctgctgca aaaagccaac gatctggggc agaagctaaa agacggattg
     1141 ttggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg agcgatgatt
     1201 gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact caccgccgag
     1261 atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc gtattacaac
     1321 gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca gggtctggag
     1381 attatcggtc agtgttttga tgaggcgaag cagtagcacc gcccccatgc aggaggcgac
     1441 gctgcgcgtc ttgtccggcc tacgagaatc caagtcggat aaggcgttta cgccgcatcc
     1501 gacaatctgt acgtgaacag gaataaatct atatcggccg gataaggcgg agccgctctc
     1561 cggcaaaaag aatcaataac aattaaacgc gtgacccgac gcgggaaatg tcggggcgct
     1621 ctcccaagtg acacactttc gagaggattc aggatggggc aatcatcgca accacacgag
     1681 ttaggcggcg ggctgaaatc acgtcacgtc acgtcacgtc acgtcacgtc acgtcacgtc
     1741 accatgttgt ctattgccgg tgttatcggc gcaagtctgt ttgtcggttc cagcgtcgcc
     1801 atcgccgaag cgggcccggc ggtattactg gcctatctgt tcgccggatt actggtggtt
     1861 atgattatgc ggatgctggc ggaaatggca gttgccacgc ccgataccgg ttcgttttcc
     1921 acctatgccg ataaagccat tggccgctgg gcgggctata ccatcggctg gttgtactgg
     1981 tggttttggg tactggttat cccactggaa gccaacatcg ccgccatgat cctgcactcg
     2041 tgggttccag gcattcccat ctggttattt tccctcgtca ttaccctcgc cttaactggc
     2101 agtaatttat taagcgttaa aaactacggc gaatttgagt tctggctggc gctgtgcaaa
     2161 gtcatcgcta tcctggcctt tattttcctt ggtgcagtcg caattagcgg tatttaccct
     2221 tatgccgaag tgagcgggat ctcaagattg tgggatagcg gcggctttat gcccaacggt
     2281 ttcggtgcgg tattaagcgc gatgttgatc accatgttct cgtttatggg cgcagaaatt
     2341 gtcaccattg ccgccgcgga atccgacacg ccggaaaaac atattgtccg cgccaccaac
     2401 tcggttatct ggcgtatttc tatcttctat ttgtgctcta tttttgtcgt cgtggcatta
     2461 ataccgtgga atatgccggg gctgaaagcc gttggatctt atcgctcggt tctggaattg
     2521 ctcaatattc cccatgcgaa attaatcatg gactgtgtga tattactttc cgtaaccagt
     2581 tgcctgaact cggccctgta taccgcctca aggatgctct actccttaag ccgtcgcggt
     2641 gatgctcccg cggtaatggg taaaatcaac cgcagtaaaa ccccgtacgt ggcggtgtta
     2701 ctctccaccg gcgcggcatt cttaacggtg gtggtgaact attacgcgcc tgcgaaggta
     2761 tttaaatttc tgatcgacag ctccggcgct atcgccctgc tggtttattt agtcatcgcc
     2821 gtttcacagt tgcggatgcg caaaattctg cgagcagaag gaagcgaaat tcgcttgcga
     2881 atgtggcttt atccctggct cacctggctg gtcatcggct ttattacctt tgtgttggta
     2941 gtgatgctat tccgtccggc gcaacagtta gaagtgatct ccaccggctt attagcgata
     3001 gggattatct gtaccgtgcc aattatggct cgctggaaaa agctggtatt gtggcaaaaa
     3061 acacccgttc ataatacgcg ctgataatga tcaggagtca caccatgacc attacgtctc
     3121 tggatggcta tcgctggctg aagaacgata ttattcgcgg taattttcaa ccggatgaaa
     3181 aattacggat gagtttactg acatcgcgtt atgcacttgg cgttgggccg ttgcgggaag
     3241 cactttctca actggtggcg gaacggctgg tcacggtggt gaatcaaaaa gggtatcggg
     3301 tggcgtctat gtcagagcag gagctgctcg atattttcga cgcccgcgcc aatatggaag
     3361 cgatgctggt cagtctggcg attgcccgcg gtggcgatga atgggaggca gacgttctcg
     3421 caaaagcgca tctgctcagt aagctggaag cctgtgacgc cagcgagaaa atgcttgatg
     3481 agtgggatct gcgtcatcag gcgtttcata cagcaattgt ggcgggctgt ggttctcact
     3541 atttgctgca aatgcgtgaa cggttgtttg atctggcggc gcgttatcga tttatctggc
     3601 tgcggcgaac ggtgctttcg gtggaaatgc tggaggataa acacgatcag caccagaccc
     3661 tgactgcggc ggtactggcg cgagatacag cgcgcgccag tgagttaatg cgccagcatt
     3721 tactgacgcc aattcccatt atccagcagg cgatggctgg caattaacta ctcttccgga
     3781 atacgcaaca cttgccccgg ataaatttta tccgggcttt ttagcatcgg tttattcgct
     3841 tcgaagattt tattgtacag attagcgtta ccgtagacct gtttggaaat ggcactcaga
     3901 gtgtcgccag acttaacggt ataaaactgg ctggcagtgg ctggtgtcgc cgttttcacc
     3961 tgatcatcaa cactggcaat accggaaata ttccccaccg caacaaggat tttctccttc
     4021 gcctcctgac tcaggccgtc accagtgacc gtcgctttgc cgtcggcaat ttgaatgttc
     4081 actttatcgg catcaggtat accggttttg ctcagatgct ccagcacctt cttcgcctga
     4141 tcgtctttat cgtgctgacc tgtaaccgcg tcccagagtt tttctccggc atctttcaca
     4201 aaattgaaca gacccatagc tacctcattc gttaacggaa acactcagaa agtgtagcag
     4261 aggaacgcgg cgcgttaacg ccgcgggggt gagcacaggt ttaatcgcgg gtttgcaccc
     4321 agaacgcgtg aatcaaaccg gggatatacc caagcagtgt cagcagaata ttaataatga
     4381 acgcccaacc gaacccttta ccgagcagca cgccgagcgg cggcagaatg atggtgatga
     4441 cgattctcca gaaacccata tgtactccct ataagaaaat tactcattgt ttaaaaagag
     4501 attttatctc ttaagcgtag taattttagc ggagcctgcc agtttcagct tacgctttta
     4561 ctctcattta cgctgctgag gctggtatat gttgcatttt tagtttagag tttacttaat
     4621 ttagaaaaca cttaaataaa tatgactgaa ctcgcgcaat tacaggccag taccgaacag
     4681 gcagcggcct tattgaaagc aatgagccac cctaaacggt tgctgattct gtgcatgctt
     4741 agcggttccc ccggcaccag cgcgggagag ctgacgcgca ttaccggact gagcgcctct
     4801 gcgacatcac agcatctcgc tcgtatgcgg gacgaagggc ttatcaacag ccaacgggat
     4861 gcccaacgca ttctatattc cattaaaaat gaggcggtaa atgccattat cgccaccctg
     4921 aaaaatgtct attgtccgta aggagtcact atggctttga caaccatttc gccgcatgat
     4981 gcacaagaat taatcgcacg cggcgcaaag ttaatcgata ttcgtgacgc tgatgaatat
     5041 cttcgtgaac atattcctga agcagatctg gctccattat ccgtgctgga acagtcaggt
     5101 cttccaccta aattacgtcg cgagcaaatt atattccact gccaggcagg taaacgcacc
     5161 agtaataacg ccgataaatt agcagcgatt gccgcccccg cagaaatatt tttactcgaa
     5221 gatgggattg atggctggaa aagagctgga ttgccagtag cggtaaataa atctcaaccc
     5281 ttgccgttaa tgcgccaggt gcagatcgct gcgggtggtt taatattaat cggcgttgta
     5341 ctgggttata ccgtaaatag cggtttcttc ttattaagtg gctttgttgg tgccgggtta
     5401 ctgtttgcag gaatcagcgg tttttgtgga atggcaaggt tgttagataa gatgccgtgg
     5461 aaccaacgag cttgagaagc gacgccggac gcgccctagc agcgacatcc ggcctcagta
     5521 attagatcag gaaatcgtcg agagatttac cttctgccag cgcctgagca attggcttcg
     5581 gtgtacggcc ctgaccggtc caggttttag tttcaccgtt aacatcagtg aatttatatt
     5641 tcgccggacg cggctggcgt tttttaccag cacgtggtgc agcagcagag ctattaccca
     5701 ataactcttc cgggttaatt ccgtcagctt tcatcagctc cagccaggtg ctaatttttt
     5761 cctgacgctc agccagttca cgctgctgct gttcttcttc ttcacgtctt tctttagtga
     5821 caaccctgaa tttttcgagc atttcttcaa gaacgtcaat ggagaattcg cgagccatcg
     5881 cacggagggt gcgaatgtta tttaaacttt gtaacattac ggacataata aagaaaacct
     5941 tttaacgcca aaacaaaaaa gtatttcgtc gcgtagtgta agcgatcact tattatattt
     6001 ccacaaaatt taatctattt acagtcctgc gcgagattat ttcagctgtt attttctgaa
     6061 actctcagaa taatttcaag attatatatt agccattttg atgagtaatt taggttattg
     6121 gtgaacagag tggcgctctc tgctcgctaa ctccgcaagc ctgacaaaac cgaagtgaat
     6181 aaaaatgcaa tataacagaa taaaagaaaa cacccctcac tggtgctttc tgctgttaat
     6241 gtacattttc tggcatcaaa aatgctgtaa atgacccggt cacgacaaac accgaattct
     6301 ctacgcttat agcgttacct cacccccaaa ctttcattag tgaaattaaa ggcaaggcca
     6361 tatccaattg atttaattga aaataaatca tatcagcatg taaaaatcca caatgtacaa
     6421 aaaacgagcc gttacggaat attttatcta caaaaactga ctaaataaaa atttttcact
     6481 aattgattag tcatagccag cgatatacgc tatgcgaaaa tgcagatggc aatgagatcc
     6541 actgctttca tctccattaa catcccatta cgcttttatt aaggagcatt agcatgtatt
     6601 aaggagcatt agcatgttct caccgcagtc acgcttgcgt catgcagttg cagatacgtt
     6661 cgcgatggtt gtttactgtt ctgtcgtgaa catgtgtatt gaagttttcc tctccggaat
     6721 gagcttcgaa cagtcttttt attccagatt ggtagcgatt ccggtgaaca tcttaattgc
     6781 atggccatac ggtatgtacc gcgatctgtt tatgcgcgcg gcacgcaaag ttagcccgtc
     6841 gggctggata aaaaatctgg ctgatatcct agcttatgtg acgttccagt caccggtgta
     6901 tgtggcgatc ttgttagtgg tgggcgcaga ctggcatcag attatggcgg cggtcagttc
     6961 aaacatcgtt gtttcgatgt tgatgggggc ggtttatggc tacttcctcg attattgccg
     7021 ccgactgttt aaagtcagcc gttaccagca ggtaaaagcc tgactcttct tttcgcgact
     7081 ggcatcgcca gtcgcgtctt taactggctt cgccaaataa accattcaaa taacgttcaa
     7141 gcgcaacacg cgaactaaag ccatgcggga taccgtaatg ctcgtgtcgc tcgcctgcta
     7201 aatagagcgg aaattgctgg taatgatcgc aagttttaat atcggaagcg ggttcagcca
     7261 gtgttgaact gcgttctttt aatgttggat gaattaccag agcggtacgt cccattcgcg
     7321 cttcgcgatt aacatagaca taattttccc cacggcggta accatacgct ttctgggtta
     7381 ccacatcgac agtaaaaccg actttttcaa gtacgcgcgc cacctcgtct ggtcgtaaat
     7441 acatatttga tcctcgttat tttcaaccgc gggcctacct tacctgattg cgcattcaca
     7501 acgctttcag aaaagtccat aaagccgcga tgaacctcag ttaagaaaat tatggtctac
     7561 actgaaaatt acatcgaatt ctaatggggg atcatatgtt taaccgaccg aaccgcaacg
     7621 acgttgatga tggcgtgcag gatatccaga atgatgtcaa tcaattagct gacagcctcg
     7681 aatctgtatt gaagtcctgg ggcagcgacg ccaaagggga agctgaagcc gcacgcagta
     7741 aagctcaggc gttgctgaaa gaaaccaggg cgcgaatgca tggtcgtact cgcgtccagc
     7801 aagccgcgcg cgatgccgtt ggctgcgcgg attcttttgt tcgtgaaaga ccctggtgta
     7861 gcgtgggtac agcagctgcg gtaggtattt ttatcggcgc actgttaagc atgcgcaaat
     7921 cgtagtgcaa aaatgataat aaatacgcgt ctttgacccc gaagcctgtc ttcggggttt
     7981 ctttttgcct ggtgaatcac aaaaatcccc ctcccccgtc acgctcatat ccagggtaat
     8041 ttcgatcact atttgctata tattgtgtgg ttgaatcttt tttcaactac atctagtatc
     8101 tctgtatcaa cagagagaca acccgacgcg tatcatcgcg ccgtatcttc attttaaacg
     8161 gaaatacgaa tcatgcgcat aactatttat actcgtaacg attgcgttca gtgccacgcc
     8221 accaaacggg cgatggaaaa ccggggcttt gattttgaaa tgattaatgt cgatcgcgtt
     8281 cctgaagcag cggaagcgtt gcgtgctcag ggctttcgtc agttgccggt agtgattgct
     8341 ggcgatctta gctggtctgg tttccgtccg gacatgatta accgtctgca tccagcgcca
     8401 caagcggcca gtgcatgagc cagctcgtct acttctccag cagctccgaa aacacgcagc
     8461 gttttatcga acgtttaggt ctgcccgcgg tgcgcatccc gctcaatgag cgggaacgga
     8521 ttcaggtaga cgagccttac atcctgatcg tgccctctta cggcggcggc ggtacggctg
     8581 gcgcggtgcc acgacaggta attcgctttt taaacgacga gcacaaccgg gcgttgcttc
     8641 gcggcgttat tgcttctggt aatcgcaact ttggtgaggc gtatggccgc gccggagatg
     8701 tgattgcccg gaaatgcggc gtgccgtggc tgtaccgttt tgaactcatg ggtacgcaaa
     8761 gcgatatcga aaacgttcgt aaaggagtaa ccgaattttg gcaacgacaa ccgcagaatg
     8821 cctgacgcag gaaacgatgg attaccacgc gctgaatgcg atgcttaatc tttacgatag
     8881 cgcaggtcgc attcagttcg ataaagaccg ccaggccgtt gacgccttta ttgcgacgca
     8941 tgtgcgtccg aacagtgtga ccttcagtag ccagcagcag cgcctgaact ggctggtcaa
     9001 cgaaggttac tatgatgaaa gcgttcttaa tcgctactct cgcgactttg tcattacgct
     9061 gtttgcccac gcacacacca gcggttttcg tttccagaca ttcctcgggg catggaagtt
     9121 ttacaccagc tatacgttga agacattcga cggtaaacgt tatctggaag attttgccga
     9181 tcgagtcacg atggtggcgc tgacgctggc acaaggcgat gagacgctgg cgttgcaact
     9241 gaccgatgaa atgctgtcag gacgctttca gccagccacg ccaacattcc tcaactgcgg
     9301 taagcagcag cgcggcgaac tggtttcctg ttttttgctg cgtattgaag acaatatgga
     9361 gtcgattggt cgggcggtaa attccgcact gcagctgtcg aaacgcggcg gcggcgtagc
     9421 atttttgctg tcgaatctgc gggaagcggg cgcgccaatt aaacgtattg aaaatcaatc
     9481 ttctggcgta attccggtga tgaaaatgtt ggaagacgca ttttcctatg ccaaccaact
     9541 cggcgcgcgt cagggggcag gtgcagtcta tttacatgct catcatcccg atattctgcg
     9601 ttttctcgac acgaaacggg aaaatgccga cgaaaaaatc cgcattaaaa cactgtcgct
     9661 gggcgtggtg atcccggata tcactttcca tctggcaaaa gagaatgcgc agatggcgct
     9721 gttttcgccc tatgacgttg agcgagttta tggcaagccg tttgccgata tcgccattag
     9781 cgaacactat gacgaactgg ttgccgatga acgcatccgc aaaaaatacc tcaacgcccg
     9841 tgatttcttc cagcgactgg cagaaatcca gtttgagtcc ggctatccct acatcatgta
     9901 tgaagacaca gtaaaccgtg ctaaccctat cgccgggcgc ataaatatga gtaatctctg
     9961 ctcagaaatt ttgcaggtta acagcgcctc agagtatgac gagaatctcg actatgcccg
    10021 cacaggccat gatatttcct gcaatttagg ttcgttgaat attgcgcaca ccatggattc
    10081 ccccgatttt tcccgcacgg tagagactgc cgtgcgcggt ttaacggcgg tgtcagatat
    10141 gagtcatatc cgcagcgtgc cgtccatcga agccggaaat gccgcctcgc acgccatcgg
    10201 actggggcag atgaatttac atggttatct ggcgagggaa ggcatcgctt atggttcgcc
    10261 ggaagccctg gatttcacca atctctattt ctataccatc acctggcacg cactgcgtac
    10321 ctcgatgttg ctggcgcgcg aacgcggtga aaccttcgcc gggttcaaac agtcacgcta
    10381 tgccagtggt gaatatttca gccaatatct gcaaggtaac tggcagccga aaacggcgaa
    10441 agttggcgaa ctgtttgccc gcagcggtat tacgttacct acccgtgaga tgtgggcgca
    10501 actgcgcgac gacgtgatgc gctacggcat atacaaccag aatctccagg cggtgccgcc
    10561 aaccgggtct atctcttata tcaaccatgc tacgtcgagt attcatccga ttgtggcgaa
    10621 agtagagata cgcaaagagg gcaaaacagg acgcgtttac taccctgccc cgtttatgac
    10681 taacgaaaat ctggcgctgt atcaggacgc ttacgaaatt ggcgcagaaa agatcatcga
    10741 cacctacgcg gaagcgactc gccatgtcga tcaggggctg tcgctgacgc tgtttttccc
    10801 cgataccgcc accactcgcg atatcaacaa agcgcagatt tacgcctggc gcaaaggtat
    10861 caaaacgctc tattacatcc gcctgcgtca gatggcgctg gaaggcactg aaattgaagg
    10921 ctgcgtctcc tgtgcacttt aaggaatatc tatgaaactc tcacgtatca gcgccatcaa
    10981 ctggaacaag atatctgacg ataaagatct ggaggtgtgg aatcgcctga ccagcaattt
    11041 ctggctacca gaaaaggtgc cgctgtcgaa cgatattccc gcctggcaga cattaactgt
    11101 cgtagaacaa caactgacga tgcgcgtttt tactggcctg acgctgctcg acacgctgca
    11161 aaatgttatc ggcgcgcctt cgctgatgcc cgatgcgctc acgcctcatg aagaggcggt
    11221 attatcgaat atcagcttta tggaagcggt tcatgcccgc tcttacagtt cgattttctc
    11281 gacgctgtgc cagaccaaag atgtcgatgc cgcctacgcc tggagtgaag aaaacgcacc
    11341 gttgcagcga aaagctcaga ttattcagca acattatcgc ggtgatgatc cgctgaaaaa
    11401 gaaaatcgcc agtgtgtttc ttgaatcttt tttgttctat tccggtttct ggctgccgat
    11461 gtatttttcc agccgcggaa agctgaccaa taccgcggac ctgatccgtc tgattatccg
    11521 cgatgaagca gtccacggtt actacatagg ctataaatat cagaaaaaca tggaaaagat
    11581 atctctggga caacgtgaag agttgaagag tttcgccttc gatttgttgc tggaactcta
    11641 cgacaacgag ttgcaataca ccgatgagct gtacgccgaa accccgtggg ctgacgatgt
    11701 gaaagcgttt ctctgttaca acaccaataa ggctttgatg aatctgggct acgaaccgtt
    11761 atttcccgca gaaatggcgg aagtgaatcc ggcaatcctc gccgcgcttt cgccgaatgc
    11821 cgatgaaaat cacgatttct tttccggttc aggctcctct tatgtgatgg ggaaagcggt
    11881 tgaaacagaa gatgaagact ggaatttctg agggtgttat tttcaaaaat atcactaccc
    11941 gcagcaggga aataattccc gccaaatagc tttttatcac gcaaatattc tgtagtgatc
    12001 tacactgata ctctgttgca ttattcgcct gaaactacaa tattcaggcg ttttttcgct
    12061 atctatgaca aaaaatatca acttttctcg atttgctctc agcccttata tcacggaaaa
    12121 ttccggcgat ttgctcgcat caatattcat gccacatttg ccctcagggg ttgcctcaga
    12181 ttctcagtat gttagggtag aaaaaagtga ctatttccat tgggtcatat atcgacataa
    12241 acaaataaag gaatcattct attgcatggc cattaaatta gaaattaaaa atctttataa
    12301 aatatttggt gagcatccac agcgagcgtt caaatatatc gaacaaggac tttcaaaaga
    12361 acaaattctg gaaaaaactg ggctatcact tggcgtaaaa gacgccagtc tggccattga
    12421 agaaggcgag atatttgtca tcatgggatt atccggctcg ggtaaatcca caatggtacg
    12481 ccttctcaat cgcctgattg aacccacccg cgggcaagtg ctgattgatg gtgtggatat
    12541 tgccaaaata tccgacgccg aactccgtga ggtgcgcaga aaaaagattg cgatggtctt
    12601 ccagtccttt gccttaatgc cgcatatgac cgtgctggac aatactgcgt tcggtatgga
    12661 attggccgga attaatgccg aagaacgccg ggaaaaagcc cttgatgcac tgcgtcaggt
    12721 cgggctggaa aattatgccc acagctaccc ggatgaactc tctggcggga tgcgtcaacg
    12781 tgtgggatta gcccgcgcgt tagcgattaa tccggatata ttattaatgg acgaagcctt
    12841 ctcggcgctc gatccattaa ttcgcaccga gatgcaggat gagctggtaa aattacaggc
    12901 gaaacatcag cgcaccattg tctttatttc ccacgatctt gatgaagcca tgcgtattgg
    12961 cgaccgaatt gccattatgc aaaatggtga agtggtacag gtcggcacac cggatgaaat
    13021 tctcaataat ccggcgaatg attatgtccg taccttcttc cgtggcgttg atattagtca
    13081 ggtgttcagt gcgaaagata ttgcccgccg gacaccgaat ggcttaattc gtaaaactcc
    13141 tggcttcggc ccacgttcgg cactgaaatt attgcaggat gaagatcgcg aatatggcta
    13201 cgttatcgaa cgcggtaata agtttgtcgg cgcagtctcc atcgattcgc ttaaaaccgc
    13261 gttaacgcag cagcaaggtc ttgatgcggc gctgattgat gcgccgttag cagtcgatgc
    13321 acaaacgcct cttagcgagt tgctctctca tgtcggacag gcaccctgtg cggtgcccgt
    13381 ggtcgacgag gaccaacagt atgtcggcat catttcgaaa ggaatgctgc tgcacgcttt
    13441 agatcgtgag ggggtaaata atggctgata aaaataatcc gtgggatacc acgccagcgg
    13501 cggacagtgc cgcgcaatcc gcagacgcct ggggtacacc gacgactgca ccggctgacg
    13561 gcggtggtgc tgactggctg accagtacgc ctgcgccaaa cgtcgagcat tttaatattc
    13621 tcgatccgtt ccataaaacg ctgatcccgc tcgacagttg ggtcactgaa gggatcgact
    13681 gggttgttac ccatttccgt cccgtcttcc agggcgtgcg cgttccggtt gattatatcc
    13741 tcaacggttt ccagcaattg ctgctgggta tgccagcgcc agtggcgatt atcgttttcg
    13801 ctctcatcgc ctggcagatt tccggggtcg gaatgggcgt ggcgacgctg gtctcgctga
    13861 ttgccatcgg cgcaatcggt gcctggtcgc aggcaatggt gactctggcg ctggtgttaa
    13921 ctgccctgct gttctgtatc gtcatcggtt tgccgttggg gatatggctg gcgagaagtc
    13981 cgcgagcggc gaaaattatt cgtccactgc ttgatgccat gcagaccacg ccagcgtttg
    14041 tttatctggt gccaatcgtc atgctatttg gtatcggtaa cgtgccgggc gtggtggtga
    14101 cgatcatctt tgcgctgccg ccgattatcc gtctgaccat tctggggatt aaccaggttc
    14161 cggcggatct gattgaagcc tcgcgctcat tcggtgccag cccgcgccag atgctgttca
    14221 aagttcagtt accgctggcg atgccgacca ttatggcggg cgttaaccag acgctgatgc
    14281 tggccctttc tatggtggtc attgcctcga tgattgccgt cggcgggctg ggtcagatgg
    14341 tacttcgcgg tatcggtcgt ctggatatgg ggcttgccac cgttggcggc gtcgggattg
    14401 tgatcctcgc cattatcctc gatcgcctga cgcaggccgt tgggcgcgac tcacgcagtc
    14461 gcggcaaccg tcgctggtac accactggcc ctgttggtct gctgacccgc ccattcatta
    14521 agtaactctg cacttgcccg gtgacgccgg gcattatcac cctgccaaaa aaaggaataa
    14581 caatgcgaca tagcgtactt tttgcgacag cgtttgccac gcttatctct acacaaactt
    14641 ttgctgccga tctgccgggc aaaggcatta ctgttaatcc agttcagagc accatcactg
    14701 aagaaacctt ccagacgctg ctggtcagtc gtgcgctgga gaaattaggt tataccgtca
    14761 acaaacccag cgaagtggat tacaacgttg gctacacctc gcttgcttcc ggcgatgcaa
    14821 ccttcaccgc cgtgaactgg acgccactcc atgacaacat gtacgaagct gccggtggcg
    14881 ataagaaatt ttatcgtgaa ggggtatttg ttaacggcgc agcacagggt tacctgatcg
    14941 ataagaaaac cgccgaccaa tacaaaatca ccaacatcgc acaactgaaa gatccgaaga
    15001 tcgccaaact gttcgatacc aacggcgacg gtaaagcgga tttaaccggt tgtaaccctg
    15061 gctggggctg cgaaggtgcg atcaaccacc agcttgccgc gtatgaactg accaacaccg
    15121 tgacgcataa tcaggggaac tacgcagcga tgatggccga caccatcagt cgctacaaag
    15181 agggcaaacc ggtgttttat tacacctgga cgccgtactg ggtgagtaat gagctgaagc
    15241 cagggaaaga tgtggtctgg ttgcaggtgc cgttctccgc actgccgggc gataaaaatg
    15301 ccgataccaa actgccgaat ggcgcgaatt acggcttccc ggtcagcacc atgcatatcg
    15361 ttgccaacaa agcctggacc gagaaaaatc cggcggcagc caaactgttt gccattatgc
    15421 agttgccagt ggccgatatt aacgcccaga acgccattat gcatgacggc aaagcctcag
    15481 aaggcgatat tcagggccac gttgatggct ggatcaaagc ccaccagcag cagttcgatg
    15541 gctgggtgaa tgaggcgctg gcagcgcaga agtaattttt attcgggcgg ataaggcgtt
    15601 ttcgcctcat ccgccgctct gtacacaatg cctgatgcga cgctgacgcg tcctatcatg
    15661 cctacaaacc acatcgcaca tttcaacaag ctatctttca tcccatattc atcaacatcc
    15721 gctattattg atttccagct taatcatcac ctgatgaaca aaaataatga ctaaacctaa
    15781 tcatgagctt agcccggcgc tgatcgtgct gatgtctatc gccaccggtc tggcggtagc
    15841 cagtaactat tacgcccagc cattgctcga caccatcgcg cgtaactttt ccctttccgc
    15901 cagttcggca ggctttattg ttaccgccgc gcagttgggc tatgccgcag gtctactgtt
    15961 tcttgttccc ctcggtgata tgtttgaacg ccgccgcctg attgtctcga tgaccttact
    16021 ggcagcgggc ggtatgttaa tcactgccag cagtcagtca ctggcgatga tgatcctcgg
    16081 tacggcatta accggtttat tctcagtcgt ggcacaaatt ctggttccgc tggcagcgac
    16141 gctggcttca ccggataaac gcggcaaagt ggttggcacc attatgagcg gtctgctgtt
    16201 ggggatattg ctggcacgaa ccgttgccgg gttactggca aacctcggcg gctggcgcac
    16261 cgtcttttgg gttgcttcgg tgttaatggc actgatggcg ctggcattat ggcgtggtct
    16321 gccacaaatg aaatcagaaa cccacctcaa ctacccacag ttgttgggtt ccgttttcag
    16381 catgttcatc agcgataaga tcctgcgcac ccgcgcgttg ctgggctgca tgacctttgc
    16441 caacttcagc attctctgga cctcaatggc ctttttgctt gccgctccac cttttaacta
    16501 cagcgatggt gacattggtc tgtttgggct tgcgggagct gccggagcgt tgggcgctcg
    16561 tccggcgggc ggttttgccg acaagggcaa atcgcaccac accacaactt tcggtctgct
    16621 tctgctgtta ctttcatggc tggcaatctg gtttggtcac acttccgtac tggcgttgat
    16681 tatcggcatt ctggtgctgg acctcaccgt acagggcgtg catatcacta accagacggt
    16741 aatttatcga atacatcctg atgcgcgtaa tcgcctgacc gcaggttaca tgaccagcta
    16801 ctttattggc ggtgccaccg gttcgctaat ttcagcctca gcctggcaac atggcggttg
    16861 ggctggcgtt tgtctggctg gcgcgacgat tgccctggtt aacttactgg tctggtggcg
    16921 aggttttcat cgtcaggaag ccgcaaatta agcaatcagc aaccgttttc gtggctttac
    16981 acttataagg gtgttaagaa gcccatcagt ctgataaggt taagatattc attcagtcta
    17041 tttataatat taacaatcgt taagcgtaca ctctatggaa agccctactc cacagcctgc
    17101 tcctggttcg gcgaccttca tggaaggatg caaagacagt ttaccgattg ttattagtta
    17161 tattccggtg gcctttgcgt tcggtctgaa tgcgacccgt ctgggattct ctcctctcga
    17221 aagcgttttt ttctcctgca tcatttatgc aggcgcgagc cagttcgtca ttaccgcgat
    17281 gctggcagcc gggagtagtt tgtgggttgc tgcactgacc gtcatggcaa tggatgttcg
    17341 ccatgttttg tatggcccgt cactgcgtag ccgtattatt cagcgtctgc aaaaatcgaa
    17401 aaccgcccta tgggcgtttg gcctgacgga tgaggttttt gccgccgcca ccgcaaaact
    17461 ggtacgcaat aatcgccgct ggagcgagaa ctggatgatc ggcattgcct tcagttcatg
    17521 gtcatcgtgg gtatttggta cggtaatagg ggcattctcc ggcagcggct tgctgcaagg
    17581 ttatcccgcc gttgaagctg cattaggttt tatgcttccg gcactcttta tgagtttcct
    17641 gctcgcctct ttccagcgca aacaatctct ttgcgttacc gcagctttag ttggtgccct
    17701 tgcaggcgta acgctatttt ctattcccgt cgccattctg gcaggcattg tctgtggctg
    17761 cctcactgcg ttaatccagg cattctggca aggagcgccc gatgagctat gaggttctgc
    17821 tgcttgggtt actggttggc gcggcgaatt attgcttccg ctatttgccg ctgcgcctgc
    17881 gtgtgggtaa tgcccgccca accaaacgtg gcgcggtagg tattttgctc gacaccattg
    17941 gcatcgcctc gatatgcgct ctgctggttg tctctaccgc accagaagtg atgcacgata
    18001 cacgccgttt cgtgcccacg ctggtcggct tcgcggtact gggtgccagt ttctataaaa
    18061 cacgcagcat tatcatccca acactgctta gtgcgctggc ctatgggctc gcctggaaag
    18121 tgatggcgat tatataaccc acaagaatca tttttctaaa acaatacatt tactttattt
    18181 gtcactatcg ttactatatc ggctgaaatt aatgaggtca tacccaaatg gatagttcgt
    18241 ttacgcccat tgaacaaatg ctaaaatttc gcgccagccg ccacgaagat tttccttatc
    18301 aggagatcct tctgactcgt ctttgcatgc acatgcaaag caagctgctg gagaaccgca
    18361 ataaaatgct gaaggctcag gggattaacg agacgttgtt tatggcgttg attacgctgg
    18421 agtctcagga aaaccacagt attcagcctt ctgaattaag ttgtgctctt ggatcatccc
    18481 gtaccaacgc gacgcgtatt gccgatgaac tggaaaaacg cggttggatc gaacgtcgtg
    18541 aaagcgataa cgatcgccgc tgcctgcatc tgcaattaac ggaaaaaggt cacgagtttt
    18601 tgcgcgaggt tttaccaccg cagcataact gcctgcatca actctggtcc gcgctcagca
    18661 caacagaaaa agatcagctc gagcaaatca cccgcaaatt gctctcccgt ctcgaccaga
    18721 tggaacaaga cggtgtggtt ctcgaagcga tgagctaacg cgtcatctcg ctcaaaaatc
    18781 cagatttata aaagaaaaaa tgaccggcca gcatcgcaac atgctggcct ttttggcaag
    18841 caggtcggct cagccgatga gttaagaaga tcgtggagaa caatatgagc gcaaatgcgg
    18901 agactcaaac cccgcagcaa ccggtaaaga agagcggcaa acgtaagcgt ctgctcctcc
    18961 ttctcacctt gctctttata attattgccg tagcgatagg gatttattgg tttttggtac
    19021 tgcgtcattt cgaagaaacc gatgacgcat acgtggcagg gaatcagatt caaattatgt
    19081 ctcaggtgtc tggcagcgtg acgaaagtct gggccgataa caccgatttt gtaaaagaag
    19141 gcgatgttct ggtcactctc gacccgacag atgctcgcca ggcgtttgaa aaagccaaaa
    19201 ctgcactggc ttccagcgtt cgccaaaccc accagctgat gattaacagc aaacagttgc
    19261 aggcgaatat tgaggtgcaa aaaatcgccc tcgcgaaagc acaaagcgac tacaaccgcc
    19321 gtgtgccgct gggcaatgcc aacctgattg gtcgcgaaga gctgcaacac gcccgcgacg
    19381 ccgtcaccag tgcccaggcg caactggacg tcgctattca acaatacaat gccaatcagg
    19441 cgatgattct ggggactaaa ctggaagatc agcctgccgt gcaacaggct gccaccgaag
    19501 tacgtaacgc ctagctggcg ctggagcgta cccgtattgt cagtccgatg accggttatg
    19561 tttcccgtcg tgccgtgcaa ccgggtgcgc aaattagccc aacgacgccg ctgatggcgg
    19621 tcgctccagc caccaatatg tgggtggatg ccaactttaa agagacgcag attgccaata
    19681 tgcgcatcgg tcagccggtc accatcacca ccgatattta cggcgatgat gtgaaataca
    19741 ccggtaaagt ggttggtctg gatatgggca caggtagcgc gttctcactg cttccggcgc
    19801 aaaatgcgac cggtaactgg atcaaagtcg ttcagcgtct acctgtgcgt atcgaactgg
    19861 accagaaaca gctggagcaa tacccgctgc gcattggttt gtccacgctg gtgagcgtca
    19921 ataccactaa ccgtgacggt caggtactgg caaataaagt acgttcaaca ccggtcgcgg
    19981 taagtaccgc gcgtgaaatc agcctggcac ctgtcaataa actgatcgac gatatcgtaa
    20041 aagctaacgc tggctaatcc agaggtgcgt gtgatgcaac agcaaaaacc gctggaaggc
    20101 gcgcaactgg tcattatgac gattgcgctg tcactggcga cattcatgca ggtgctggac
    20161 tccaccattg ctaacgtggc gatccccact atcgccggga atctgggctc atcgctcagc
    20221 cagggaacgt gggtaatcac ttctttcggg gtggcgaatg ccatctcgat cccgcttacc
    20281 ggctggctgg caaagcgcgt cggggaagtg aaactgttcc tttggtccac catcgccttt
    20341 gctattgcgt cgtgggcgtg tggtgtctcc agcagcctga atatgctgat cttcttccgc
    20401 gtgattcagg ggattgtcgc cgggccgttg atcccgcttt cgcaaagtct attgctgaat
    20461 aactacccgc cagccaaacg ctcgatcgcg ctggcgttgt ggtcgatgac ggtgattgtc
    20521 gcgccaattt gcggcccgat cctcggcggt tatatcagcg ataattacca ctggggctgg
    20581 atattcttca tcaacgtgcc gattggcgtg gcggtggtgt tgatgacact gcaaactctg
    20641 cgcggacgtg aaacccgcac cgaacggcgg cggattgatg ccgtggggct ggcactgctg
    20701 gttattggta tcggcagcct gcagattatg ctcgaccgcg gtaaagagct ggactgtttt
    20761 tcatcacagg aaattatcat tcttaccgtg gtggcggtgg tggctatctg cttcctgatt
    20821 gtctgggagc tgaccgacga taacccgata gtcgatctgt cgttgtttaa gtcgcgcaac
    20881 ttcaccatcg gctgcttgtg tatcagcctc gcgtatatgc tctacttcgg cgctattgtt
    20941 ctgctgccgc agttgttgca ggaggtctac ggttacacgg cgacctgggc aggtttggcc
    21001 tctgcgccgg tagggattat tccggtgatt ctgtcgccga ttatcggccg cctcgcgcat
    21061 aaactggata tgcggcggct ggtaaccttc agctttatta tgtatgccgt ctgcttctac
    21121 tggcgtgcct atacctttga accaggtatg gattttggcg cgtcggcctg gccgcagttt
    21181 atccaggggt ttgcggtggc ctgcttcttt atgccgctga ccaccattac gctgtctggt
    21241 ttgccaccgg aacgactggc ggcggcatcg agcctctcta actttacgcg aacgctggcg
    21301 gggtctatcg gcacgtcgat aaccacgacc atgtggacca accgcgagtc gatgcaccat
    21361 gcgcagttga ctgagtcggt aaacccgttc aacccgaatg cccaggcgat gtacagtcaa
    21421 ctggaagggc ttgggatgac gcaacagcag gcatcaggct ggattgccca gcagatcacc
    21481 aatcaggggc tgattatttc cgccaatgag atcttctgga tgtcagccgg gatattcctc
    21541 gtactgctgg ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg cggcggaggc
    21601 ggtgcgcact aagtacaact aagccagttc atttgaactg gcttttttca attaattgtg
    21661 aagatagttt actgactaga tgtgcagttc ctgcaacttc tctttcggca gtgccagttc
    21721 ttcgttgctg ttgatgcgta cgtcacgctc cagaatgcta cgcgcaatat cctgcgcttc
    21781 ctgcaacgag tgcatctggt aagtgccaca ctggtagacg ttcagttccg ggatctgatt
    21841 ctgatcctgc actttcagca cgtcttccat tgccgctttc caggcatcag caacacgctg
    21901 ctcatctggc gtaccaatca gactcatata aaaaccggtg cggcagccca ttggcgagat
    21961 atcgataatc tctacaccat taccgttaag atggttacgc ataaaaccag caaacaggtg
    22021 ctccagggta tggatccctc tttctggcat cacttctttg ttcggcacgc agaagcgcag
    22081 atcgaacacg gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca cccgaactgc
    22141 aggcgcttcc atccgggtat gatcgactgt gaagctatct aacaacggca tttagccacc
    22201 tccggtaatt tttttaaaaa ttttctgaac tctttcttcc caggcgagtc tgagtatatg
    22261 aaagacgcgc atttgttatc atcatccctg aattcagaga tgaaattttg gccactcacg
    22321 agtggccttt ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa cggttcggta
    22381 tcagcggttt ccatttcctg ctgacggcgt tcagatgcct cgcgctcggc tacaaaatcc
    22441 tcttcccgca gaatttccag cggctcttca cgcagcagat tacggtaggc ttctgcaaac
    22501 gctttgcctg ttccgccaat accagtatca atcatagacc ttaagatacg ggcagagaaa
    22561 gtcagatcgg gattatcgaa gcaggcaacc agttcatcac acactttctg atacgcttcg
    22621 ccgccgttaa tgctatccag cgtttgcgcg acgcgtttca gatcgcggaa cagatcttta
    22681 cccacctgca gtaacgggaa ctgcgcggtt tcgcagccga tacccagcgt caggcccggt
    22741 ttgcgacctt caaggatcac ccggttccag ttaacgcgcg tacaggcaag ttcgctactg
    22801 ctcatttccg gtgcatcagc cagcgcacac cagaccataa acaggtcgag gaatcgcacc
    22861 tgctgttcat ctacaccaat cggcgagaac gggttgatgt ccagcgaacg cacttcaata
    22921 tattcaatgc cgccacgtaa cagcgcatca gaaggtaact cgccgctgcg ggtaacgcgt
    22981 tttggacgaa tcggcgcata cagttcgttt tcaatctgca gcacgttgct attgatttgc
    23041 agcctcttac cgtctttctc aataccaatc ttcgcgtact cttccgatgg cgttttgatt
    23101 gcctgtttaa ggcccgctac gtactcgtaa agatcgttga aggtaatacc aagattgctt
    23161 tgcgatttat tggtatagcc gagatcgctc aaacgaagag aggtcgcata cggcaggtaa
    23221 tacataccgc actcggtttt ctcaaacggc agcgacgttg gttttccttg caggaaagaa
    23281 gaacaaatcg ccggagatgc accaaacaga taaggaatga cccaaccgaa acgatagtaa
    23341 ttgcggataa cgcggaaata gcccgcagaa attttctctt tggcatcagc gcccgagata
    23401 tcaccgcact tcgcttgcca gaatgccatt ggcaaagaga aattgtagtg cacgccggaa
    23461 atggtttgca tcagcgcgcc gtagcgattt ttcagccctt cacgatacag cgttttaaag
    23521 cgtccggtgt tagaagtgcc gtactgtgcc agttcgatgt cctgaccttc tgcgatgtag
    23581 catggcatac ttaacggcca catccgctca tcgcccatat tgcgcgccgt ataacgatgc
    23641 agatcgcgca taaaggtcag catatgttca atatcaccat ccactggtgt aatgaattcc
    23701 agcaatgctt ccgcaaaatc ggtagtaatc catttgtgcg tcagtgcgga acctaatgct
    23761 tcaggatgac ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt ttcgcgctcc
    23821 agcccacgct gtatcccctt taacgcctga ggatgttttt ccagccaggc cagcgcctgt
    23881 gatacgtccg ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt agcataactg
    23941 taatggtgac catatgtgca ggcctacaat tagtgccacc acatcatgcc ctgaacggtc
    24001 gctgctgcaa ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa gattactggc
    24061 ccccaggaga tgcgcatcca tcccgctaac agacacagta aatcgccaac cacgggcatc
    24121 cagcttaata atagcgtgac tgcaccatag cgtttcagcc agccggtagc tttctcttgc
    24181 cagcgcgatg ttttacgcaa tggaaagaaa cgcccaagga taacgttagt taaccctcca
    24241 aggctattac ccattgttgc tgttaaaact aaaacccagg gatgactgat cccggaaagc
    24301 aacattgcca ccagcacgac ttcggagttg ccgggtaata gtgtagcgct gagaaaacta
    24361 ctggcaaaca acgagaaaag cgataacgct tcactcacag caagcgaaca tccacagcgt
    24421 ccatgcctgc tgcacgggcc gcctgaatac cgaaatcggc atcttcaaag accacacact
    24481 gcgtcggttg cacgcccata cgctgcgcgc acaacaaaaa tgtgtctggc gcgggtttat
    24541 ggtgtttgac gtgatcagca gcgacgacgg cgtcaaaata acggcgtaat cccaggtgcg
    24601 ccagcaatgc ctcagcgatg gcgctttcac tccccgttcc tacagccatt gggcgacgac
    24661 catgccaact tttcaccact tcaacaagag gaagcggctc gacactatcc agcagcatac
    24721 ttcttactgc ttctgttttt tcacgcgcta acgcatgcgg gtcgagatcg gcctgattca
    24781 gctcaataat tgcctgagca atacgccagg tgggcgatcc attaagcgca atcatcgcct
    24841 gaatatcgta ctgaagaccg tagtgcccta atacttcgcg ccacgcttta cggtgcgtag
    24901 gctccgtatc caggattgtg ccatccatat caaaaattaa acctgcataa cgctcgtaca
    24961 tggtcttctc gcaagtcgaa aaatatgacg ctactttagc gtaattgctt gattttgtcg
    25021 ctgatggcga aggagaagaa aattcaggaa atggataaag tagttattta gaaatgagat
    25081 atttttgaag gaaatttttt ggagaatatg gtgcatccgg gaggattcga acctccgacc
    25141 gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcatcgg gaaatttatt
    25201 ttactgctga tattgatacc gctactaaag ccatatcaag taagagatgg tgcatccggg
    25261 aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc tgagctacgg
    25321 atgcatcggg aaacttactt tactgcagat tttttgatac cgctactaaa gccgtatcaa
    25381 gtaagagatg gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta
    25441 ctctatccag ctgagctacg gatgcaaatg gcggtgaggc ggggattcga accccggatg
    25501 cagcttttga ccgcatactc ccttagcagg ggagcgcctt cagcctctcg gccacctcac
    25561 cacacgcctc ttacgagtgc ttcgaagaac ttgtttatcg ctcatcgtcg ctgcgtggcg
    25621 cacatattac tttctgagac ttataagtca aacaattttt cccacacttt tatcgtttgc
    25681 acacttcacg ctcaattagt ctgtaaaaac gtcaaaaaga gtgttttatc tacagaaaaa
    25741 cggaggtctg gcagatagca gtaatgcaaa aaaatggaga cttaagttga atgaacggga
    25801 gtaaagcgaa aagactatag agtgaaggag aaattttgag ggtgcgtctc accgataaag
    25861 atgagacgcg gaaagattag taactggact gctgggattt ttcagcctgg atacgctggt
    25921 agatctcttc acggtgaaca gaaacttcct tcggggcatt tacgccaata cgtacctggt
    25981 tgccctttac ccctaaaact gtcacggtga cctcatcccc aatcatgagg gtctcaccaa
    26041 ctcgacgagt cagaatcagc attctttgct ccttgaaaga ttaaaagagt cgggtctctc
    26101 tgtatcccgg cattatccat catataacgc caaaaagtaa gcaatgacaa acacattaca
    26161 tgtaagcagt catggcatta cattctgtta aacctaagtt tagccgatat acacaacttc
    26221 aacctgactt tatcgttgtc gatagcgtta atgcgaatgc cgtgaagcga gtccacggca
    26281 ttgcctgacg cttatattat tgcaatttcg cgctgaccca gcctttcaca ctggctaacg
    26341 ctgcaggtaa ggccgcagca tccgtaccac cggcttgcgc catgtcagga cgtccaccac
    26401 ccttgccgcc cacctgctga gcgaccatac caatcagttc ccctgctttc acacgatctg
    26461 tgacgtcctt agatacgcct gcaatcagag aaaccttacc ttcggttacc gttgccagca
    26521 cgataattgt cgaccccagc tgatttttta aatcgtcaac catggtacgc aacattttcg
    26581 gctcaacacc gctaagctcg ctaaccaaca gcttaacacc attaacatca attgccttac
    26641 tggaaagatt tgcgctctcc tgtgcggcag cttgttcttt aagctgttgt agttcttttt
    26701 ctaactgacg cgtacgtccc agtactgagc gcactttatc cgccagatta ttgctatcgc
    26761 ctttcagcag atgcgcgact tcgcttaagc gatcgctgtc tgcatgaacg gtggtgatag
    26821 cgccttctcc ggttaccgct tcgatacgac gaacgcctgc agcagtaccc gattcagaga
    26881 tgatgcggaa cagaccaata tcaccagtgc ggctggcgtg agtaccgcca cacagctcgg
    26941 tggagaaatc gcccatgctc agcacgcgta cacgctcatc atacttctcg ccgaacagcg
    27001 ccatcgcacc tttcgctttc gccgcttcga gatccatgat gttggtttcg atcggcaaat
    27061 tgcgacgaat ctgtgcgttc accaggtctt cgaccgcacg aatctcttcc tgtttcatcg
    27121 cttcgttgtg tgagaagtcg aagcgcagca ccttgtcgtt aaccagtgaa cctttctgcg
    27181 atacatgagt ccccagaacc tggcgcagcg cagcgtgcat caggtgcgtt gcggagtgat
    27241 tcagacgaat acgggcgcga cgagcctcat caatatcagc ctgcacagcg tcgcccactt
    27301 tcagagaacc cgcagcaagt ttaccgatgt gaccaatcgc ctggccgtat ttctgcgtat
    27361 cttccaccgc gaaggagaag ttagcgcctt tcagttcgcc tttatcacca acctgaccgc
    27421 cggattccgc atagaatggc gtttgatcca gcacgaccac agcttcctgg cctgcattga
    27481 tggcatcaac cgctttacca tcaacaaaca gcgcggtcac tttgccgttc agttccagat
    27541 ggtcatagcc tttaaattca gatgcactat caacacggat cattgcgttg taatcggcac
    27601 caaagccgct ggcttcgcgc gcgcgacgac gctgctcttc cattgctgct tcaaaaccag
    27661 cttcgtcaac tttgatgttg cgctcacgac aaacatcagc cgtcaggtca accgggaagc
    27721 cataggtgtc gtacagacgg aaagccgttt caccatccag cgtatcacca gaaagttttg
    27781 ccagctcttc atccagcaac gccagaccgc gctccagagt acgggcaaac tgctcttctt
    27841 cagtcttcag cacctgctca acctgcgcct gctggcgttt caggtcttca cccgcagagc
    27901 ccataacgtc gatcagcgga ccaaccagtt tgtagaagaa ggtttctttc gcgccgagca
    27961 tattgccgtg acgcactgcg cgacgaatga tacgacgcag tacataacca cggttttcat
    28021 tggacggcat tacgccatcc gcgatcagga acgcacaaga acgaatgtgg tcagcgatta
    28081 cgcgcagcga tttattgctc agatcggtcg cgccagtgac tttcgctacc gcctggatca
    28141 acgtgcggaa caggtcgatg tcatagttag agttaacgtg ttgcagcacc gcagcaatac
    28201 gctccagacc cataccggta tctacagacg gcttcggcag cggttccatc gtgccatctg
    28261 cctggcggtt gaactgcatg aagacgatgt tccagatctc aatgtagcgg tcgccgtctt
    28321 cttccggact tcccggaggg ccaccccaaa tgtggtcgcc gtgatcgtag aagatttcgg
    28381 tgcacgggcc gcacggacca gtgtcaccca tctgccagaa gttgtcagat gcgtatggcg
    28441 cacctttgtt atcgccgatg cgaataatac gttcgcgcgg gatcccgact tctttttccc
    28501 agatttcgta ggcttcgtcg tcgctttcat agacggtaac ccacagacgc tctttcggca
    28561 gggcaaacca tttttcgctg gtcagcagtt cccatgcaaa ctgaatggca tcgtgtttga
    28621 aatagtcgcc gaagctgaag ttgcccagca tttcgaagaa ggtatggtga cgcgcggtgt
    28681 aaccgacgtt ttccaggtcg ttgtgtttac cacccgcacg cacgcagcgt tgggaagtgg
    28741 tagcgcggga ataattacgc ttgtcgagcc caaggaacac atccttgaac tggttcatcc
    28801 cggcgttggt aaacaacaaa gttgggtcgt tatggggtac cagggagctg ctggcaacta
    28861 cctgatgtcc cttactatgg aaaaagtcga gaaacgcctg acggatctca gcggtgctct
    28921 tgctcataat tatcctgaaa tcaagctaac gaaatatcgc caccagctcc agcgtgtctt
    28981 aaccgccggg ctggtaactg aaaagtggga ataagataag ttttcttgac tgggaagtaa
    29041 aataccgtat gcgttcagtc ggcaaaattt cgccaaatat cctggatatc ttccatcaga
    29101 tagccacgat agagcagaaa acgctggatc ttaacttttt ctgaaaagac agttggcaaa
    29161 ggttcaccat attttcgcgt cgcctgatcg cgcgccagtg cgcaccagtc gatgtcacat
    29221 tcacgcatcg ctttttctgt cgcttcgcgg gaaatacctt tctgattcag ttcctgacga
    29281 atacgtgcag gtccataacc tttgcggcta cggctggcga taaagcgcgc aacaaatcgg
    29341 ctgtcatcga gatagccatg ttcatggcac caggcaataa cgcgctcgta atcttctgcc
    29401 gtagcatcaa tctcttctgg gccatttttg cccataatcg gtgccgcgag tttacgtcgc
    29461 agttcttgct cactgtgatc gcgcaccgcc agaatgcgta ccgcacgatc caacaggcga
    29521 gcatatgccg ggcgacggga tgttgattct gtcatggcat atccttacaa cttaaaaaag
    29581 caaaagggcc gcagatgcga cccaggtttt gctgataaac agatttaata gtcaatgagt
    29641 atgcagaatg ttcccgcagg cccaattcgt tagaggatcg aggtgagagt aatttttcac
    29701 gccagtgctg gcctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtc
    29761 cgtgcacaca ttatctatta tcatcaataa cgaacgatta aaaatcttcg ttagtttctg
    29821 ctacgccttc gctgtcatct acagagaaat ccggcgttga gttcgggttg ctcagcagca
    29881 actcacgtac tttcttctcg atctctttcg cggtttccgg gttatctttc agccaggcag
    29941 ttgcattcgc tttaccctga ccgatcttct cacctttgta gctgtaccac gcgcctgctt
    30001 tctcgatcag cttctctttt acgcccaggt caaccagttc gccgtagaag ttgatacctt
    30061 cgccgtagag gatctgaaat tcagcctgtt taaacggtgc agcgattttg ttcttcacca
    30121 ctttcacgcg ggtttcgcta cccaccacgt tttcgccctc tttcaccgcg ccgatacgac
    30181 ggatgtcgag acgaacagag gcgtagaatt tcagcgcgtt accaccggta gtggtttccg
    30241 ggttaccgaa catcacacca attttcatac ggatctggtt gatgaagatc agcagcgtgt
    30301 tggactgctt caggttaccc gccagcttac gcatcgcctg gctcatcata cgtgccgcaa
    30361 ggcccatgtg agagtcgccg atttcgcctt cgatttccgc tttcggcgtc agtgccgcca
    30421 cggagtcaac gacgataacg tctactgcgc cagaacgcgc cagggcgtca cagatttcca
    30481 gtgcctgctc gccggtgtcc ggctgggagc acagcaggtt atcgatatcg acgcccagtt
    30541 tacgtgcgta gattgggtcc agcgcgtgtt cagcatcgat aaacgcacag gttttacctt
    30601 cacgctgcgc tgcggcgatc acctgcagcg tcagcgtggt tttaccggaa gattccggtc
    30661 cgtagatttc gacgatacgg cccatcggca gaccacctgc cccaagcgcg atatccagtg
    30721 aaagcgaacc ggtagagatg gtttccacat ccatggaacg gtcttcaccc aggcgcatga
    30781 tggagccttt accaaattgt ttctcaatct ggcccagtgc tgccgccaac gctttctgtt
    30841 tgttttcgtc gatagccatt tttactcctg tcatgccggg taataccgga tagtcaatat
    30901 gttctgttga agcaattata ctgtatgctc atacagtatc aagtgttttg tagaaattgt
    30961 tgccacaagg tctgcaatgc atacgcagta gcctgacgac gcactgcatc acggtcgccg
    31021 ctgaagcatt cccgccgggt aatgccttca ccgcgggcag tggcaaaagc aaaccagacg
    31081 gtgccgacag gcttctcttc actgccgcca tccggcccgg cgataccact aatagacacg
    31141 gcataatcag cacgagccgc tttcagtgca cctatcgcca tttccaccac gacgggttca
    31201 ctcaccgcgc catgctgcgc cagcgtctct tcgcgtacgc cgatcatctg cgctttggct
    31261 tcgttactgt aggtgacaaa tccgcgttca aaccaggcgg agctaccggc aatatcggta
    31321 atcactttcg ctacccaacc accggtacaa gactcggcag ttgttacggt tgcgccacgg
    31381 gctttcagcg cctgcccaac ctgttcgctt aactgcatca gttcactgtc agtcatcaca
    31441 acctctgtca gtcaaaaatt tatgccggac aagatagcac tttcgtcagg aagatggtgt
    31501 tggcgttgca attttacgag gaggattcag aaaaaagctg attagccaga gggaagctca
    31561 cgcccccctc ttgtaaatag ctactgtact cgcgccagcg ccacggcttg tcctaactgc
    31621 cagaccgcca ttgcgtaatg ggtgctgtgg ttgtaacggg tgatggtgta gaagttcggc
    31681 agaccgtacc agtactggta gccggtgcca acatccagac gcagcaggct ggcttgttga
    31741 tggttgccca gcggctgctg tggcgttaaa cctgcggcgg caagctgcga aatgctgtac
    31801 ttagttttga aaccatttgg caagcctgga gcctgaccgt ttgccattat cgcgacctga
    31861 tcgcctttca cccagccgtg cgctttgaaa tagttcgcca cgctaccgat cgcatcaacc
    31921 ggatcccaca ggttgatatg cccgtcgccg ctgaaatcta ccgcatattg tttgtaagac
    31981 gacggcataa actgtccgta gcccatcgcc ccggcaaagg aacctttcag attgagcgga
    32041 tcgtcctgct cgtcgcgcgc catcagcagg aaggtttcca gctcgccaga gaaatactcc
    32101 gcgcggcgtg ggtagttaaa tgacagcgtt gccagcgcat cgaggatgcg agttttcccc
    32161 atcacgcgcc cccagcgggt ttcaacgccg ataatcccga cgataatttc cggcggtacg
    32221 ccatacacct gccacgcgcg attcaacgca tcttcatact gattccagaa aaccacaccg
    32281 ttctgcacgt tgtccggcgt aataaatttt ttgcgataac gcagccatgc gccgttcgga
    32341 cctgatggcg gtttcaccga tgtggttggt gcctggttat ccatcagccg cagtaccgaa
    32401 tccagacgct tcgcctggga gagaatttcc tgcaactgct gacgatcgaa accgtgtttg
    32461 ttcaccattt tgtcgatgaa ctgctgggca ttcgggttat tagcgaaatc gccgcccatc
    32521 tgcatcacat tgtgctgcgg ctcaagcagg aagccgccag acggcgttcc ggtggtcgtt
    32581 tcagtctcag taggttttgg cttgctgcta caggcggcaa gcaacacaaa aaggggaagc
    32641 aatgttacat aacgacgctt gaacatgagg ggtccattta acagattcaa ccaagggcaa
    32701 gtatggtaaa gcatcacgcc ccgcacaagg aagcggtagt cactgcccga tacggacttt
    32761 acataactca actcattccc ctcgctatac ttttattcaa actttcaaat taaaatattt
    32821 atctttcatt ttgcgatcaa aataacactt ttaaaccttt caatctgatt atattaggtt
    32881 gccatttggt aataaaacaa taaatcctga aggagagaac aatgatagaa accattactc
    32941 atggtgcaga gtggtttatc gggctgttcc aaaagggcgg agaggtgttt accgggatgg
    33001 tgaccggcat tcttccgctg ttgattagcc tgctggttat catgaacgca ctgattaatt
    33061 ttatcggtca gcatcgtatt gaacgttttg ctcaacgttg cgccggtaac cctgtttccc
    33121 gttacctact gttaccgtgc attggcacgt ttgtcttttg caatccgatg accttaagcc
    33181 tgggtcgctt tatgccggaa aagtacaaac ccagctacta cgcggcggcc tcttatagct
    33241 gccactcaat gaatggcctc ttcccccata tcaaccctgg cgaactgttt gtttatcttg
    33301 gcattgccag cggtctgaca acgctgaacc tgccacttgg cccactggcg gtgagttatc
    33361 tgctggttgg tctggtcacc aatttcttcc gcggctgggt gaccgatctg accaccgcca
    33421 tttttgagaa aaagatgggc attcaacttg aacaaaaagt tcacctggca ggagcaacat
    33481 catgacgcgt attcggatcg aaaaaggaac gggtggctgg ggcggcccgc ttgagctgga
    33541 agccacgccg ggcaaaaaaa tcgtctatat caccgccggt acccggcctg cgattgttga
    33601 caaactggca caacttactg gctggcaggc tattgacgga tttaaagaag gtgaacccgc
    33661 ggaggcggaa attggtgtcg tggtaatcga ctgtggcggc acattacgct gcggcatcta
    33721 tccgaaacga cgtattccca ccattaatat ccactcgacg ggcaagtccg gtccgctggc
    33781 gcagtacatt gtggaagata tttatgtctc tggcgtaaaa gaagaaaaca tcactgtagt
    33841 gggtgatgcg acaccacaac cctcttccgt gggccgtgac tatgacacca gtaagaaaat
    33901 caccgaacaa agcgatggtt tactggcgaa ggtgggaatg ggcatggggt ccgccgttgc
    33961 cgtgttgttt cagtctggtc gtgacaccat cgacactgta ttaaaaacca ttctgccgtt
    34021 tatggcattc gtctcggcgc tcattggcat cattatggct tctggccttg gtgactggat
    34081 tgcccacggt cttgctccgc tggcgagcca tccactgggt ctggtcatgc tggcgctcat
    34141 ctgctccttc ccactgcttt cacctttcct cggcccaggc gcagttatcg cacaggttat
    34201 cggcgtattg attggcgtgc agattggtct cggcaatatt ccgccgcatc tggctttacc
    34261 ggcactgttt gccatcaacg cgcaggcggc ctgcgacttc atcccggtcg gtttgtcgct
    34321 ggcggaagcc cgtcaggaca cggttcgcgt cggtgtccct tctgtactgg tgagccgctt
    34381 tttaaccggc gcgccgactg tactgatcgc ctggtttgtc tccggtttta tctatcaata
    34441 gaggctgaaa catgaccgtt atttatcaga ccaccatcac ccgtatcggc gcgagtgcca
    34501 ctgacgccct cagcgaccag atgctcatca cctttcgtga aggcgcgcca gcggacctcg
    34561 aagagtattg cttcattcat tgccacggcg agttgaaagg tgcactccat cccggtttgc
    34621 aattttcact cgggcagcat cgctatccgg tgaccgctgt tggcagcgtg gcggaaaaca
    34681 accttcgcga actgggtcat gtcaccctgc gcttcgatgg tttaaacgaa gcggaatttc
    34741 cgggcactgt ccatgtggca ggccctgtac ccgacgatat cgcgccggga tcggttttga
    34801 agtttgaatc tgttaaggag taaaaaatga atcaggttgc cgttgtcatc ggtggtgggc
    34861 aaaccttagg cgcgttcctg tgccacggtc tggctgccga ggggtatcgc gtcgcggttg
    34921 tcgatattca gagcgacaaa gccgcaaatg tggcacaaga aattaacgcc gaatatggtg
    34981 aaggtatggc gtacggtttt ggtgctgacg ccactagcga gcaaagcgtt ctggcgctct
    35041 ctcgtggggt agatgaaatc tttggtcgcg tggatttgct ggtctatagc gccggaatag
    35101 caaaagcagc ctttatcagc gacttccagc tcggcgattt tgaccgttcg ctacaagtga
    35161 atctggtggg ttatttcctg tgtgcccgtg aattttcgcg tttgatgatc cgcgacggga
    35221 ttcagggtcg cattattcag atcaactcga aatccggcaa agtgggcagc aaacacaact
    35281 ctggctacag cgcagcgaaa tttggtggcg tcgggctgac tcaatcactg gcgctggatc
    35341 tggcggagta cggcattacg gtgcattcac tgatgctcgg taacctgctg aaatcgccga
    35401 tgttccagtc actgttgcca caatacgcga ccaagctggg tatcaaaccg gatcaagtcg
    35461 agcagtatta catcgacaaa gtaccgctca aacgcggctg cgattatcaa gatgtgctga
    35521 atatgctgct gttctacgcc agtcctaagg cgtcgtactg caccggacag tcgatcaatg
    35581 tcaccggcgg tcaggtgatg ttctgatcaa cagcggagat ccgttaagga tctccgtgag
    35641 actatagaat gcctgatgcg ctacgctcat caggcataca ggacttccgc cactacatta
    35701 aggaaaagtt atggtatccg cactcatcac cgtcgccgtt atcgcctagt gtgcgcaact
    35761 ggccttaggc ggctggcaaa tttctcgttt taaccgtgcc ttcgacacac tatgccagca
    35821 agggcgggtt ggcgtgggcc gttccagcgg gcgctttaaa ccgcgggtcg tggtcgccat
    35881 cgcgctggac gatcagcagt gcatcgtcga caccttgttt atgaaaggac tgactgtctt
    35941 cgcccgaccg caaaaaattc ccgcaattac cggcatgcat gcgggtgatt tacagcccga
    36001 tgtgatcttt ccccatgatc cactatcaca gaatgctcta tcattggcgc ttaaactgaa
    36061 acgtggataa tttcgttgtg aatgttactt gcttgcgaag ttatcatttt gaaacctaaa
    36121 tcaggtaatc acgcccatga aacctcgtca gcgtcaggcc gccattctgg agtatctgca
    36181 aaagcagggt aaatgctcgg ttgaagaatt ggcgcaatac tttgacacca caggcacaac
    36241 cattcgcaaa gatctggtca ttctggaaca tgccggaacc gtcattcgta cttatggcgg
    36301 agtggtgttg aataaagagg aatccgatcc gcctatcgat cataaaacgc tcatcaacac
    36361 ccacaagaaa gagctgattg cagaagctgc cgttagtttt atccatgatg gcgattcgat
    36421 cattcttgat gctggcagta ccgttttgca gatggttccc ctgctctcgc gctttaataa
    36481 catcacggtg atgaccaaca gcctgcatat cgtcaatgcg ctatccgaac tggataacga
    36541 acaaactatc ctgatgccag gcggaacatt tcgcaaaaaa tcggcctcat ttcacgggca
    36601 actggcagag aatgccttcg agcatttcac cttcgataaa ttgtttatgg gcaccgacgg
    36661 catcgatctc aatgcgggcg taaccacgtt caacgaggtt tataccgtca gtaaggcaat
    36721 gtgcaatgcc gcgcgcgaag tgattttgat ggcggactca tcaaagtttg gccgtaaaag
    36781 ccccaacgta gtttgcagtc ttgaaagcgt cgataagcta attaccgacg caggtatcga
    36841 tccggcgttt cgtcaggcgc tggaagagaa agggatcgat gtgatcataa ccggagagag
    36901 caatgagtga agcactactg aacgcgggac gtcagacgtt aatgctggag ttgcaggaag
    36961 caagccgttt accggaacgt ctgggcgatg attttgttcg cgccgccaat atcatcctgc
    37021 actgcgaagg caaagtggtg gtttcgggaa ttggcaaatc gggccacatt ggtaagaaaa
    37081 tcgccgcaac acttgccagt accggcacac cggctttttt tgtccatccg gcagaagcac
    37141 tgcacggcga tctggggatg attgaaagcc gcgatgtgat gctgtttatc tcttactccg
    37201 gtggcgcgaa ggaactggat ctgattattc cgcgtctgga agataaatct atcgcgctgc
    37261 tggcgatgac cggcaaaccg acgtcaccgc tgggcctggc ggcgaaagcg gtgctggata
    37321 tctccgtaga acgcgaagcc tgcccgatgc accttgcgcc gacctccagc accgtcaata
    37381 ccctgatgat gggtgacgcg ctggcgatgg cggtcatgca ggcgcgcggt tttaatgaag
    37441 aagattttgc ccgctcccac ccagctgggg cactgggcgc tcgcttgctg aataaagtgc
    37501 atcatctgat gcgtcgtgat gacgctatcc cacaggtagc gttaaccgcc agcgtgatgg
    37561 atgcgatgct ggaactcagc cgcaccggtc tggggctggt ggcggtatgt gacgctcaac
    37621 aacaggtaca aggcgtcttt accgacggcg atttacgtcg ctggctggta ggcggcggcg
    37681 cactcaccac gccagttaat gaagcgatga cgacaggcgg caccacatta caggcacaaa
    37741 gtcgcgccat cgacgccaaa gaagttctga tgaaacgcaa aatcactgcc gcaccagtgg
    37801 tagatgaaaa cggcaaactc accggcgcaa ttaacctgca ggatttctat caggccggga
    37861 ttatttaatc cttcaatccc agacgtttcg ccagccgatg caggttggcg acgtcagttt
    37921 ccagcattcg cgcacaggct gcccagttgt gatgattttg tgccagtgcc tggcgaatag
    37981 tttcacgctg gaacgcttct gtcgcttcac gcaggttttg cttaacaacg ggcaccgccg
    38041 ccgcttctgg cggcggcaac gtcacctcag gaaaagcaaa atgttgcgcc tcaagaatca
    38101 cttcatcgcc gttgcgggtg gctctcgcca gcactaccgc ccgatgaata gcatgttcca
    38161 gttcgcgcac gtttcccgga aaactgtagt gttgcagtaa atttcgcgcc ccggcactta
    38221 ataccacgcg ggagagcccc tgccgcaaac gacactgctc gcagaaatac cccgccagca
    38281 gaatgacatc atcgccccgc tcacgcagcg gcggcaccga aagtggaaac acgctcaggc
    38341 gatgaaacaa atcggcgcgg aatcgccctg ccagcacctc ttcgcgtaag tcgcggttag
    38401 tcgccgccag cacgcgcaca tcgacccgca aactacggtc atcgccaacg cgctgaatat
    38461 cgccatactg caacaccctc agcagcttgg cctgcaatgc caacgacaac tcgccgatct
    38521 catcgagaaa cagagtaccg ttatccgcca tttcaaactt cccgctgcgg ttactgatag
    38581 cgccagtaaa cgctcctttc acatgcccga acaactcact ttccgccaca ctttccggca
    38641 gtgcggcaca gttgagatag accagcggat tcaccgcccg tggcgaggct tcatgaatcg
    38701 ctttcgccac cagctcctta ccggttcccg tctcaccgct gatcagaacg ttgagatcgg
    38761 acgccgccac aatctcaatc tcttttttca gttgcgtcat gccaggggac aagccaatca
    38821 tctgcgtctg tttcaccgct tcaaacggcg tggcatcgcc tggcagcata ttctggcttt
    38881 ccagttgttc aatcagcaac gcattgctta acgctcccgc cgccagcgcg gcaatcagcc
    38941 gtaactcttc gtcgctgaaa acatcgaact gatcgggctg catcccgtcg agcgtcagtg
    39001 cgccgatcag gttttgtccg acaaacaatg gcagaccaac gcaggcgtga accttcagac
    39061 tctcctgccc aggaatcaaa ccgtcatagg gatcgggcaa ttcgctgtct gcgggaaagc
    39121 gcaccacatc cccggcgcgg gcaatcgctt ccagccgtgg atgcccttcc agcgcaaagc
    39181 gtctaccgag tacatccttt gccagaccgt cgatggcaag cggaataaac tgccgcgaat
    39241 cgtaacgtag caacgcagac gcatcgcact ccagcacctg acgtagcgtg gtgatcaggc
    39301 gctgaaaacg atcctgatga ccaatcccac gctgcaattc gatggcgata ttcgccagca
    39361 catcaacgga aaaactcatc tttgcctcac tgtcaatttg actatagata ttgtcatatc
    39421 gaccatttga ttgatagtca ttttgactac tcattaatga gcataatttt atttatagag
    39481 taaaaacaat cagataagaa actggcacgc aatctgcaat tagcaagaca tctttttaga
    39541 acacgctgaa taaattgagg ttgctatgtc tattgtggtg aaaaataaca ttcattgggt
    39601 gggtcaacgt gactgggaag tgcgtgattt tcacggcacg gaatataaaa cgctgcgcgg
    39661 cagcagctac aacagctacc tcatccgcga agaaaaaaac gtgctgatcg acaccgtcga
    39721 ccataaattc agccgcgaat ttgtgcagaa cctgcgtaat gaaatcgatc tggcggatat
    39781 cgattacatc gtgattaacc atgcagaaga ggaccacgcc ggggcgctga ccgaactgat
    39841 ggcgcaaatt cccgatacgc cgatctactg tactgccaac gctatcgact cgataaatgg
    39901 tcatcaccat catccggagt ggaattttaa tgtggtgaaa actggcgaca cgctggatat
    39961 cggcaacggc aaacagctca tttttgtcga aacaccaatg ctacactggc cggacagcat
    40021 gatgacttac ctgacaggcg acgcggtgct gttcagtaac gatgctttcg gtcagcacta
    40081 ctgcgacgag catctgttca acgatgaagt ggatcagacg gagcttttcg agcagtgcca
    40141 gcgttactac gccaatatcc tgacgccgtt cagccgcctg gtaacgccga aaattaccga
    40201 gatcctgggc tttaacttac cagtcgatat gatagcgacc tcccacggcg tggtctggcg
    40261 cgataacccg acgcaaattg tcgagctgta cctgaagtgg gccgctgatt atcaggaaga
    40321 cagaatcacc attttctacg acaccatgtc gaataacacc cgcatgatgg ctgacgctat
    40381 cgcccaggga attgcggaaa ccgacccacg cgtggcggtg aaaattttca acgtcgcccg
    40441 aagcgataaa aacgaaatcc tgaccaatgt cttccgctca aaaggcgtgc tggtcggcac
    40501 ttcgacgatg aataacgtga tgatgccgaa aatcgccggg ctggtggagg agatgaccgg
    40561 attacgcttc cgtaacaaac gcgccagtgc tttcggatct cacggctgga gcggcggtgc
    40621 ggtggatcgc ctttccacgc gcctgcagga tgcgggtttc gaaatgtcgc tcagcctgaa
    40681 agccaaatgg cgaccagacc aggacgctct ggagttatgc cgtgaacacg gtcgcgaaat
    40741 cgcccgtcag tgggcgctcg cgccgctgcc gcagagcacg gtgaatacgg tagttaaaga
    40801 agaaacctct gccacgacga cggctgacct cggcccacga atgcagtgca gcgtctgcca
    40861 gtggatttac gatccggcaa aaggcgagcc aatgcaggac gttgcgccag gaacgccgtg
    40921 gagtgaagtc ccggataact tcctctgccc ggaatgctcc ctcggcaaag acgtctttga
    40981 cgaactggca tcggaggcaa aatgagcaac ggcattgtga tcatcggttc gggcttcgcc
    41041 gcccgccaac tggtgaaaaa tattcgcaaa caggacgcca ctattccatt aaccctgatt
    41101 gccgccgaca gcatagatga gtacaacaaa cccgacctca gccatgttat cagtcagggg
    41161 caacgtaccg acgaccttac ccgccagacg gcgggtgaat ttgccgggca gtttaatctg
    41221 cgcctgtttc cgcacacctg ggtgacggat atcgatgccg aagcccatgt ggtgaaaagc
    41281 cagaataatc agtggcaata cgacaagtta gtgctggcaa ccggtgccag cgcctttgtc
    41341 ccgcctgtgc ctgggcgtga gttaatgctg acgttaaata gtcagcaaga gtatcgcgcc
    41401 tgtgaaacgc aactgcggga tgcccgacgc gtgttgattg tcggcggtgg tttgattggt
    41461 agcgaactgg cgatggattt ttgtcgtgca ggcaaagcgg tcacgctaat cgacaacgct
    41521 gccagtattc tggcgtcatt aatgccacca gaagtaagca gccgcttgca gcatcggttg
    41581 acggagatgg gcgttcatct gctgttgaaa tctcagttgc aggggctgga aaaaacggat
    41641 tctggcattc tggcaacgct ggaccaccag cgtagtatcg aagtggatgc ggtaattgcc
    41701 gccaccggac tgcgcccgga aacagccctg gcacgacgcg ccgggctgac gattaatcgc
    41761 ggcgtttgcg tcgatagtta tctgcaaacc agtaatgccg atatttacgc gctgggcgat
    41821 tgcgcggaaa ttaacggtca ggtattgccg ttcctgcagc cgattcaact tagcgggatg
    41881 gtgctggcaa aaaatcttct cggcaataac acgccgctga aactaccggc gatgctggtg
    41941 aaaatcaaaa cgccggaatt accgctgcat ctggcaggcg aaacccagcg tcaggattta
    42001 cgctggcaga ttaataccga acgccaggga atggtggcgc gcggcgttga cgatgctgac
    42061 cagcttcgcg cctttgtggt cagtgaggat cggatgaaag aggcatttgt attgttgaaa
    42121 acgttgccgg tgtaggtggg ctactgtgcc taaaatgtcg gatgcgacgc tggcgcgtct
    42181 tatccgacct acggggacgc atgtgtaggc cggataaggc atttacgccg catccggcaa
    42241 gggtgtccaa atgcaacacg ctttatccgt tctggacttc acccgctaac caacgcgccg
    42301 cagcaataac cccctgcccc agagacaaac cgccatcacc cgccggtaaa ctctgtggaa
    42361 agagcaatgt gaaatcagcg agataatgcg ccagacgtgc acgcagcaaa cggttatgaa
    42421 taaccccgcc gctaaatacc agcgtagtga taccacgcat cgtggcctgc tcacgcatca
    42481 acgcggcaaa accctgcgcc agcgcatcat gaaacgccca cgcgcgttga ttaaccggtg
    42541 ccagccagtt cagccactgc tgccagaaag tggcgagatc cagttgattg tccacccgcg
    42601 gcatcgtcac cggatgcgtc actccgtcgc atgaggctgc gagcgcctcc agagcacaag
    42661 ccgcttcacc ttcataactt aacgtggctg gcgcacagcc cagtgccgcc gccactgcat
    42721 cgaacaaccg cccacacgat gatgccagcg gcgcgttaat tccacgctca atggcccgcg
    42781 ccagtacgct ccagttttgc tgttgcacac ttgccgtttc agggtaattc tgccactccg
    42841 gcacaaagcg caggcactgc gccagcaggt ttcgccacgg ctgcttcgcc gccaaatcgc
    42901 cacccggaag cgccactgca ggcaagccgc ccaggtgctc acattcgcga tagttcaccc
    42961 gcaggcactc gccgccccac aaagcgccat tctcccccat accgataccg tcgagcgtca
    43021 aagcaatgac atcaccgcca tccagcggcc actgatgctc tgccagacac gccgctgcat
    43081 gggcatgatg atgcagtacc gtttgcgtcg gcagattcat ttcgctcgcc cactggctgg
    43141 agacatagcc cggatgcaca tcatgcacaa tgtattgcgg agtgaaatcg tagatgtttt
    43201 gcatcaggcg taacgcttcg cgccactgca tctggatgcc atcgtcactt aaatcgccca
    43261 gatgctgact caacaccgct tgttcaccgc gcaccaggca gaaggtattt ttcagatccg
    43321 cgccaagaca cagcacaggc ggaacatttt taaagcccgg aggcaaaacc agcgcatccg
    43381 gcacataccc ccgcgaacgg cgcagcattt cgccgctttc gcgcaccact gaatcatcca
    43441 tccgctgcac gatgtcgcgg ttatgtagca agaatccgtc ggcaatgccc tgcaaatccg
    43501 ccagcgcctg ttcgttgctg atagctggtg gtttaccgct caggttgccg gaggtcatca
    43561 ccagcgggca ttgcagttcc tgtaacagca aatgctggag cgggttcgca ggcaacatca
    43621 ccccgacttc gttaaggcca ggggcgatat catcacaaag ctcaggtacg tattttttat
    43681 ccaccagcac aatcggcgca gcgggcgtgg taagcaactg gcgcgcagcg tctggtaaac
    43741 tttctgccac tggcaacatg actgccagcg gtttcgccgg gcgatgtttg cgctcccgca
    43801 gtgtcgccac cgcgttactg ttacgtgcat cgcaggcaag atgaaatccg ccaatccctt
    43861 tgatggcgac aatgttgccc atttttaact gtgcgatagc tgcctgtaat gccgcctctt
    43921 gttctgcatg ttcaccatga cttacccatt caagatgcgg gccacactcc gggcaggcca
    43981 ccggctgggc gtggaagcga cgatcgagcg ggtcgcggta ctctttgtca caggccggac
    44041 atagcggaaa cgccgccatc acggtaaacg ggcggtcgta aggcatcgcg cgaataatgg
    44101 tgaaacgcgg gccgcagtgg gtacagttga taaacggata acgataacgc cgctcgcctg
    44161 gggtattcat ttcggcaagg caagcagggc aagtggcggc atccgggaca atttgcgtat
    44221 tcatggtgcc gccggtgctc tggcggatgg tgaactcggt gggcagttgt gaccagataa
    44281 acggctcacg ctcgacgcta tcaatacgcg ccagtggcgg acagtgctga tacaattgaa
    44341 caagaaacgt ttccgggtct tccagcagcc ggacttctac gccatcgccg tcattacata
    44401 catcgccgtg aagatttaat tgctgtgcca gctgccagac aaacggacga aaaccgacgc
    44461 cctgcacttt gccacgaata cgcagttgga caccgcaaga tgtgtttttt gccattgagt
    44521 tattcccgcc atcatgaatt gcgtaacccg ccctgccgga cacgacagcg tcgtatccgg
    44581 cagtctcagg tcggcgatac cgcccgctcc gtattctacg aatatttccg ggaattcctt
    44641 tgatgccaga acagttctgt aagattttta gaacatcagc gccgtacggc ggcgtttttc
    44701 tgcgctcagt tgttcaagtt tattacgatc gacacaaatc agcgcatggg tcgggcaagc
    44761 cgccatacac gccgggccgt cttcacgatg gttgcacagg tcgcatttat tggcttcggc
    44821 tttgtcagcc cgtacattca gacccgcgcc gctattgcga atcacaggac gtaccaccac
    44881 ttccatcgcg ccatacgggc aagccaccac gcaggttttg caaccaatgc aacgttcctg
    44941 catcacatga acaaaccctt tatcacggct gatagcacca ttcgggcaga cgttagcgca
    45001 cggtgcatct tcacactgac ggcaaactgt cgccgtggaa atgttcacac ctttaatgac
    45061 atggatacgc ggtaaaaatg tttccggggt cagcgatgca cagtcctgat tttcctgatg
    45121 agaaaccacg cacgccactt cacaggtacg gcaaccaata catttactcg cgtcagcaat
    45181 gatgaaacgg ttcatcaaat tctccagcaa tgacagttaa tgcgccgata cattcacaaa
    45241 tcatgccagt ttttaatttg ctgttattta aggaaattaa tttctgtaat gcaggaaaaa
    45301 agatgtcatc gacactagtg acgatgacat gtgatgacaa tgtttatcgc gaaagggcaa
    45361 tgagtgagtc gcggcggatc agttttccgc taaaggtttt cggcggtgag aaatccccgc
    45421 catcgagcat aaaaatcagc cgtccaataa tttcctgaat catctcagtc accggaattt
    45481 ttacgctgga gagcgccgga acgatatagg gggcaattgc gatatcatcg aatccgataa
    45541 ctgacacctg ctctggcacc gctacgccgc gctcgtgcaa cgctttgatc gcacctatcg
    45601 ccatatcgtc gttactggca actaacgcgc taaatttagc cccacgttcg agcaacgttt
    45661 ctaccccttc ggccccgctg gcgggcgtcc atttaccgtt agcgataagt ttttcattga
    45721 gcgcaatacc atgctgcgac agcgcgtctt tatacccggc aagacgttca atgctggtgg
    45781 gggaatccat cgagccggta aggaaagcaa tctcctgatg cccggcgttt atcaactctg
    45841 ccacggcgtt aaagctggtc tgtttatgat cgcaccagac gctatggctg ctattattgc
    45901 gcaggcggcg attaagcacc attatcggct gactgtgcgc gtcaatgatg tcatcaatct
    45961 catccacgct taaaaaacgc gggtaaatca taatggcgtc gcagcgcaaa tccagcagat
    46021 actgaatcgc ctggcgctct tcttcggcgc tgtgtttacc atctgccaat agcaactgtc
    46081 gccctttctc ttccgccatt cgcgcggcat gaaacagtaa ttcactaaaa taaatgccgt
    46141 gataaagcgt gttggtcact accagcccca gcgtctgagt actcttcgcc gacagattgc
    46201 gggccagcaa atttgggcgg taactgctct cttctaccgc ctgaaacacg cgatctttag
    46261 tctcctggct gacgtagcca ttacctgaaa gcacgcggga aacggtcgct tttgaaaccc
    46321 cggcgcgctt cgccacttcc agcatcgtcg tcatcatttt catcccttta cacgcaatca
    46381 acgcagtgta ctgcaccgtt tgccgattgt ccttgcgcaa tcggcgggaa aaatattcag
    46441 gtgaccggtt tcacaaatat aaaaaatgaa caattaactc tcttgcttat ttagtgacaa
    46501 ctattcatga ttttgtgaaa ccggtttctt aattccgttt cagcataggc atttttccgt
    46561 cacgtcgact gataacaact acatctaccc tactgataac aggataaaat ccgatggcca
    46621 aaaattatgc ggcgctggca cgctcggtga tagcggcact gggcggcgtt gataacatct
    46681 cggcagtcac gcactgtatg acgcgtttgc gctttgttat caaagatgac cagcttatcg
    46741 acagcccgac gttaaaaacc atccccggcg tgctcggcgt ggtacgtagt gacaaccagt
    46801 gtcaggtaat tatcggcaat acggtttcgc aggcctttca ggaagtcgtc agcctgctgc
    46861 cgggagatat gcagcccgca cagcccgtgg gtaaacccaa actcacgctg cgtcgcattg
    46921 gtgcggggat cctcgatgcg ctgatcggca ccatgtcacc gctgatcccg gcgattatcg
    46981 gcggatcgat ggtcaaactg ctggcgatga tcctcgagat gagcggcgtg ctgacaaaag
    47041 gatcgccgac cttaaccatt ctgaatgtga ttggtgacgg tgctttcttc ttcctgccgc
    47101 taatggtcgc ggcatctgcc gccatcaaat ttaaaaccaa tatgtcgctg gcgattgcca
    47161 tcgcgggtgt gctggtacat ccgagtttta ttgaattaat ggcgaaagcg gcccagggtg
    47221 aacatgtcga atttgccctg atcccggtca ccgcggtgaa atacacctac acggtgatcc
    47281 cggcgctggt gatgacctgg tgcctgtcat atatcgaacg ctgggtggat cgcattacac
    47341 cggcggtgac caaaaacttc ctcaagccaa tgctgattgt gttgattgcc gcaccgctgg
    47401 caatcctgct gattggcccg attggtatct ggatcggtag cgccatttcg gcgctggttt
    47461 acaccattca tggttatctg ggctggcttt cagtcgccat tatgggcgcg ctgtggcctc
    47521 tgctggtaat gaccgggatg caccgcgtct ttacgccaac catcattcag accattgccg
    47581 aaaccggcaa agaaggaatg gtcatgccat cagagatcgg cgctaacctg tcgctgggcg
    47641 gttcatcact ggcggtgccg tggaaaacga aaaacccgga actgcgccag acggcgctgg
    47701 ctgcggcggc atcagccatt atggcgggga tttccgaacc ggcgttatac ggcgtggcga
    47761 tccgcctgaa acgtccgctt atcgccagtc ttatcagcgg ttttatttgc ggcgcggttg
    47821 ccggtatggc ggggcttgcc agccactcaa tggcagcgcc ggggctattt accagcgtgc
    47881 aattcttcga tccggcgaat ccaatgagca tcgtctgggt gttcgcggtc atggcgctgg
    47941 cggtgatgct gtcgtttatc ctcacactgt tgctcggctt tgaggatatt cctgttgagg
    48001 aagcggctgc ccaggcgcga aagcatcaga gcgtacaacc gaccgtcgcc aaagaagtaa
    48061 gtcttaattg aggatgaaaa tgtcagtatt tccagaaggt tttttatggg gcggcgcgct
    48121 tgccgccaac cagtctgaag gtgcgttccg tgaaggcgga aaagggctga ccaccgtcga
    48181 tatgatccca cacggcgagc atcgaatggc ggtgaaactg gggctggaaa aacgttttca
    48241 gttgcgcgat gacgagtttt atcccagcca tgaggcgacg gatttttatc atcgttataa
    48301 agaagatatc gccctgatgg cagagatggg attcaaggtt ttccgtacct caattgcctg
    48361 gagccgtctc tttccgcagg gcgatgaaat cacgcccaat cagcagggca ttgcttttta
    48421 tcgtcctgtc tttgaagagt gtaaaaagta cggtatcgaa ccgctggtca cgttgtgcca
    48481 cttcgatgtg ccgatgcatc tggtcaccga atatggctcc tggcgtaacc gcaagctggt
    48541 ggagtttttc agccgctacg ccagaacctg ctttgaagca tttgatggtc tggtgaaata
    48601 ctggctaacc ttcaatgaaa tcaacattat gttgcatagc ccgttctccg gcgcgggtct
    48661 ggtgtttgaa gaaggtgaaa atcaggatca ggtgaaatat caggccgcgc atcaccagct
    48721 ggttgccagt gcgctagcca ccaaaatcgc ccatgaggtt aacccgcaaa atcaggtggg
    48781 ctgtatgctg gcgggcggta acttctaccc ttacagctgc aaaccggaag atgtctgggc
    48841 ggcgctggag aaagatcggg aaaacctgtt ttttatcgat gtgcaggcgc gcggcgcgta
    48901 tccggcttac tctgcccgcg tatttcgcga aaaaggggta accatagata aagcgctggg
    48961 cgatgatgag atcctgaaaa acaccgtcga ttttgtctct ttcagctatt acgcctcgcg
    49021 ctgcgcctcg gcggagatga acgccaacaa cagcagtgcg gcgaacgtgg tgaaatcgct
    49081 gcgtaacccg tatctgcaag tgagcgactg gggctgggga attgatccac tcggtctgcg
    49141 tatcaccatg aatatgatgt acgatcgtta tcagaagccg ctgtttctgg tggaaaacgg
    49201 cctcggcgca aaagatgaac ttgctgccaa tggcgagatt aacgatgact atcgcatcag
    49261 ctatttacgc gaacatatcc gcgcaatggg cgaagcgatt gcagatggca ttccgctgat
    49321 gggctacacc acatggggct gtattgattt agtttccgcc tctacgggtg aaatgagcaa
    49381 acgctacggt tttgtctacg tagaccgtga cgacgcaggc aacggcacgc tgacgcgcac
    49441 acgtaagaaa tcgttctggt ggtataaaaa agtgattgcc agtaatgggg aagatttaga
    49501 gtaagtaaca gtgccggatg cggcgtgaac gccttatccg gcctacatag ttcgtgcgac
    49561 atgtaggccc gataagcgca gcgcatcggg caatttagca tttggcatta gtcaaaaatc
    49621 aaagaaacca ttccggctta acgccaaatc gggctgaaag tgccttaatg tgggatatcg
    49681 tcagggatcg ttgtccagac aaaatctgac tgaccaggga tttagagccg atttcgttct
    49741 tcaaatccgc ataggtcaga ttatgttgat caatcagcgt gcgaagtaac gccacgccaa
    49801 caggcattgc cgcaacggct ttgtcgaact ctgcaaactc ttggttgttg ttttcatatt
    49861 ctgcaatgcg gctcgccaga aagtcgataa gtggattggt gtcatcttct tcaatcaaat
    49921 attcgaccag ttccagtgct tcacgataat catcttctga gtcactacct cccagaaagg
    49981 ggactgcatt gaccagttct cttgttgctt taacagcgcg tgcagcatta gcagtcattc
    50041 tttgttctct ctgtagtaac gtgtcagttt gtcatactcg gcgtgattag ttatgtgctt
    50101 cacaaagaac cgcttattaa caaagtttat gtaggcaatc acccttaggt tgttcccacc
    50161 tacatccaga acgtaccatt tgttgcgata tttaaaattg tcgaggttgg gaaacgccgt
    50221 tcgcatctct tctggcgtag aaaaatccgt ttccttaata acccgataca atgcctgaag
    50281 agccagagcg tcgttaggat atttacgcgc agactcttca aatggcgctt tagaaatgat
    50341 gtgcattttt aagttttaac ttccttatag taagtaaaca gccacatctg cgagttctat
    50401 ccttatgtgt tcggaaacta tagctcatca aatccaagtt cacaatatgt aaacacttgt
    50461 gcgcgatata attaccccca aaatcctcat agccactctg caaaaagtca gttctggttc
    50521 acccctcaag cacatccccc tttatcaagc aacgctgcgt ctgcgccaga tcttccgttt
    50581 gcgggtcgcg cccggcgatg gggttgagga cgagtttacg gttgggggta taagtaaaac
    50641 gcgtgagcgc gggatagctt aacttgtcaa accagccgtg aacacgctgc catttggcgt
    50701 cttttacctg atacagactg gttatccagt aaccagaatt aaactgcata tccagcaatt
    50761 gcgtgtgacc attgccttgc aaatccagca aatcatctac gccagttttg cttgcggtgt
    50821 agaatcccct gggttcaaag acacagggac ggccttcgcg cgtgaacgtc ataagaatta
    50881 aatgtgcata aggagccaga ccattaccgg tacttggtaa ccagataacc agataaccag
    50941 atcctgaatg ccgttacgat caagatcggc gcgatagagt tgccctttgc ccgctatgtt
    51001 tgccagcata cccacaggca cagtccagga tttaccggcg cggtctttag cggttaattg
    51061 ccagtaatct tcttcatttg gcatcttctc aagcgtcacg ccagcaacgc ccactttcct
    51121 aaaatgccag gtttgctcgg catcataagg catggtcagg agaggtttca gcgcccgctt
    51181 ttgcgcgtat tcgctgtcac tcattggcag atattcggta aagctggtgg cctgtgcagg
    51241 cagcactaag ctcaataaaa aagcgcacat ccctgttctg atgttcaatt ccctgaattc
    51301 cctgatggag aattattctt cttccaccgc taactgcgca aatccaccat ttccttccca
    51361 gccttccagt cgttgataaa cggtttctac cgcatcttta atcggctggg tcatcgggta
    51421 gtaaaagccg acgatatccg gctgaatgcc gaggaaaatc acttcaccaa tgtcttcttt
    51481 taactggtcg ataaggtaat tcaacggcat gttatgggta gtcatcataa acatctcggc
    51541 gatatcatcc gggtcgatga tgcggatttc gccggggttt agccccatat ccgtggcgtc
    51601 gacaatcagc agtcgtgtcg ggcgcagttc acggatggcg acgatgtcgt tttccggtgc
    51661 actaccgccg tcaatcacca cccagttacc tttcggcgcg gcggcgcact tttccgccag
    51721 cagcgggcca gcgccatcat cgcccatcat gctattgcca acacagagta aaacgtcagt
    51781 cacgcagtct cctcaccatc aaatagatgg cgttttcctg atgaatatca tgcagcatac
    51841 ttaacagcat tgtactccat tcctgctgct gcgaagtttg cgctgcccgg gctttatcga
    51901 atgcgttggc cagcatcggc acatggttaa tgtcgatgac gatctcgcca tatttcggta
    51961 cgccttccat tttgtggcgg gcttcgctgc ccgcctccag agtggcaatc catgccagat
    52021 attcatccca tgggcaggtg agcgccgctt ccaggcaatc gataacccca aggtggtgac
    52081 caatcgccag gctgtaatag accacctgct gcgcctcggc gggcgtggca tcgttctcat
    52141 caataaattt acggctcagt tgactgaaca ccaccttttc actcatcgga tacgcgcctc
    52201 ttcaacaaca tgattcagat ggctgacaat ctcgttcaga cgcggatcgt tttccgcttc
    52261 cagccagcgt gcaacctgtt cttcgccctg ccctaactgt gtaaggtaat catcggcaat
    52321 ctgacgaccg taacgataac ccgccaggcg acgtgcttcg cgatccactt tcacgcgcag
    52381 cggctgcacc atatcgccat gcaggatctc cgccggttgt tcatccagtt cacccggccc
    52441 acgggcgtga attttctgct ccagcaggcc gagcgccatt gcaaagccgt acagcgtggc
    52501 ggcaggcgtt ggcgggcagc cagggatata aacatccaca gggacaattt tatccgtacc
    52561 gccccacacg cagtagagat cgtggaagat cccgccgctg ttaccgcagg caccgtatga
    52621 gatacagatt ttcgggtccg gcgcggactg ccacgcacgc agcgcagggg atcgcattgc
    52681 acgggtgacc gcgccggtaa acagtaaaat atccgcatga cgcggtgaag gaacgacttt
    52741 aatgccgaag cgttctgcat cgaacagcgg agaaagcgtg gcgaaaattt cgatttcgca
    52801 gccgttgcag ccgccgcagt ccacgcggta aacataggca gaacgtttga tttttttcag
    52861 taacgacgcc ttcatgctgg cgatggattc atccaccgtc atcgggaccg gaatgccgtt
    52921 ggcgtcacgg gggcctaata aattgctcat cagatggcct ctttcatatg gcgagtcagt
    52981 tcaatacggt cggacggcac caggcatttc tggcgcttac attccgggca agtctcaaag
    53041 ctttcgcggt ggttttccgc gcggctgtca ccattgtgct taagcagcgc aatggcgtag
    53101 tcgatctctt tctgtacggc gaaaggacga ttgcagacgc ggcagttgca cagcgcgaag
    53161 cgggactgtt gcaggaagtc ttctttcttc cacaccgcca gttcgtactc ttgcgacagt
    53221 ttgatcgccg ccgtcgggca aacttcttcg cagcgtccac agaagatgca gcgcccgaga
    53281 ttaaactccc acgccagctc tcctgtggcg aggtcagttt caaccgttaa ggcgtttgac
    53341 gggcaggcat tgacgcaagc cgcgcagccg atgcactgct gcgggttctg ctctggctta
    53401 ccacggaagt ttttatcaac cgcaatcggc tccagcggat aagacgaggt cgccgtgccg
    53461 gttttgatga cttttttgat aaaggtaaac atggcgattc cttatttcag cggcgagttt
    53521 ttacgctcaa tgctgtaacg ctcgagttct ttgtacggca ccactttgct cttcttctta
    53581 cgcacatcga ctacggtcat gcggtcggta caggagtagc aagggtccaa gctaccgata
    53641 atcagcggcg catcggaaac ggtattgcca cgcagcatgt agcgcagggt cggccagttc
    53701 gcgtaggtcg cggcacggca gcgccagcgg tacagcttct ggttgtcgcc ggtcatgctc
    53761 cagtggatat cgtcgccgcg cggcgcttcg gcaaagccca gcgcgaaacg gtgcggaatg
    53821 taggtaaagc cttccaccat cagcggaccg cccggcaggt tatccagacc gtagtcgatc
    53881 atattcagcg cggtatagac ctcgttgatt cgcactttca gacgggaaat aacgtcgcag
    53941 ccctgctcgc tgtggacttc cattggcagc aggccataac cgacaaacgg gtgatcggcg
    54001 cgagtatcgc gggcgagacc gctggcgcga accatcgggc caacgttact gaaatcgcgg
    54061 gcgatttccg ggtccagacg accaatgccg acggtgcgtt gttccatgtt cggcgtactc
    54121 agcagcacat ccaccagctc ctgcacttca cgacgcattt gttgcgccag ctggcgggtc
    54181 tggatcatgt catctttcag cagatcgcga cgaatcccgc cgatcaggtt caggccgtag
    54241 gttttacgcg caccggtaag gatctctgcc attttcatgg aggtttcacg cacgcggaag
    54301 aactgcataa agccggagtc gaagccggta aagtgacagg cgaggccgag gttgagcaga
    54361 tgcgagtgca ggcgttccac ctccagcaga atggcgcgga tcatctgcgc gcgttctggc
    54421 acctgaatac ccatcgcgtt ttccaccgac gtggtgtagg cggtgctgtg ggcaaagccg
    54481 cagatcccgc acacacggtc agagaggaag gtcacttcgt tataacccat gcgggtttcc
    54541 gccagttttt ccataccgcg atggacgtag aacagacggt agtcggcgtc gataatgttt
    54601 tcgccatcga cgaacagacg gaagtgaccc ggttcgtcag aagtgacgtg cagcggacca
    54661 atcggcacga cgttgttttt cttgtcgccc agttcgttga tgaactcgta ggtttcagca
    54721 tcggtagtcg gtgccggacg ctgacgataa tccatgctgt ctttacgcag cggataaagt
    54781 tcatccggcc agtcatccgg cagcaccaga cgacgttcat ccggcagacc aaccggaatc
    54841 aaaccgtaca tatcgcgcac ttcgcgctcg ccccacaccg ccgccggaac gcgcggcgtc
    54901 acggacggat actccggttt gttggcgtca acttcgacgc gaaccgttac ccaacacttg
    54961 gtgcccttct ccatcgacag cacgtaataa acggcgtaat gaccattcag tttgcgttcg
    55021 tcgttaccaa acagcaccga cagccagcca ccctgcttgt agtagagaaa ctccaccact
    55081 tccggcagat agttcacctt tacggtgaca gtcagctgat ctttggtctg ccaggcgtgg
    55141 tccagcacga cgcctggaaa tgcctcattc agcgcggcga gataatgttg acctaatttt
    55201 tcttcagaca tgctcaaact ctctttaatc acgccgccag caaggagacg aacgctaaaa
    55261 atgcaaagcc aaacccagcc caggtaatgc gcggagtaat atcaagacgc agacgggcca
    55321 tgctgttttc gaacagcgcg ataaccagga cgccgaccac cagtttgacg atggcaatca
    55381 ccagcgccag cagcagtcca ccgacggtga aggtttccat ttgtccccac ggaataaaca
    55441 ccccgacgaa catctgcaac accaccagct gtttcaggct gataccccat ttcatgacgc
    55501 caaagccgct accgctgtat tcggagagcg gaccttcctg caactcctgc tcggcttccg
    55561 ccaggtcgaa cggcagtttg cccatttcga taaaagtggc gaacgcacag gcgcagagcg
    55621 ccagcaccag cgggatgctt tggctcagcg gccagtgata aacggtgtcg gtaatgttgc
    55681 tgatgttggt ggaaccggca acctgtgcgg caacccacag accaagcagc aacatcggtt
    55741 cgaccagcac gccaagcatc gcttcacggc tcgcaccgat agcggtaaac gggctaccgg
    55801 tatccagacc ggaaatggca aagaagaaac gcgcgatggc gaagagatac agtaaggtga
    55861 tcaaatcgcc cagttgcggc agcggagaac cgacggtcac caccggcagc gcagtagcga
    55921 tagtcagcat gacgcccacc atcacatacg gcgtcaggcg gaacacccag ccagaggcat
    55981 ccgggccaac gctctggcgg cccagcagtt tgataatgtc gcgatactcc tgcaacacgc
    56041 ccggcccgcg acggttatgc agacgggcgc gcgccacgcg ggtaataccg gagagcagcg
    56101 gcgcaacggc aaataacacc agcgcctgaa ttaacggata taaaacactc attctcaggc
    56161 tcctcgtgaa acaataatca ccaccagtac cgccagttca accagcgcca tccggcggaa
    56221 cagcaacgca ctaccttcgc actgccagcc cggcaccagc gacaccggat tcagccattt
    56281 gcgcagtttc agcactggcg caaacgcctg tttaaccggc atggcaaaac cgtgagcggt
    56341 aatcaccatt gatttttcat ggtcgtagcc gcacacccag gccgcaccgc gggaacgtga
    56401 cggcaggcgg tcgcctttga aaatcgccat aatgatgaat ggcagcagcg ggcaggcaat
    56461 cagcagcaat gtgatcattg gttgagaaac ggtggtgtta gcaggctcca gaggcagagg
    56521 tacagcagca gagagcatcg gcagcagcca cggcgcagca acaccgccaa ttacgcagca
    56581 aatcgccagt gccactacgc ttacgctcat caggagcggc gcacaggtgg cgttttcggc
    56641 ttctttggta cgcggcgcgc cgaggaacgt gacgccatag actttcgcca tacacatcac
    56701 cgccagcgca ccggtaattg ccagacccac agcgagcagc ggccccagca gacgggcaac
    56761 aaacgcgcca ctattgctca gtttgaagaa ggattgatag ataacccatt ccccggcaaa
    56821 cccgttcagt ggcggcagcg cagccattgc catcaacccg actaacatgg cgatggagat
    56881 aaccggcatt ttcttgccaa taccaccgag tttttcgata tcgcgatgac cggtacggaa
    56941 ccagacgctc cccgccccga ggaacagtac gcttttgaac aggctatggt taagcagatg
    57001 gtacagacca ccgaccaggc caagagcaat cagcgccggt tgttcgagcg cgatacccgt
    57061 tacgccagcg cccagcccca gcaggataat gccgatattt tccagggtgt ggtaagccag
    57121 caggcgctgg atgttgtgtt ccatcaacgc atacagacca ccgacgaacg cggtgatcat
    57181 gccgagcacc agcagcgcga tcccccacca cagcggtgca ttaccgccca gcagtgacag
    57241 ggttaacatg cccagcaggc caattttcat gactaccgta gaaaacaacg cggcagctgg
    57301 cgcagaggcg ttagcatgtg cctgcggcac ccagccgtgc agcggaataa tcccagccag
    57361 caggccaaag ccaatcacac cgagcagcca gatatcggaa ccgagcggca gctgttgcat
    57421 acgcatatcc agcaggcgca gatccagcgt gccataacgc tgccacagca gccagcaagc
    57481 aatcaccagc agcagagtgc caagacgccc cagcgcaaac cacagtttgc cctctttgct
    57541 gttgctggtg aggaacaccg cacacaaggc catgacttcg gccattacca cgaacatgcc
    57601 gaggttgctg gcgatgacgg cgcagacggc ggcagccatc aacatattga tctgcaagcc
    57661 gttgcacttc acctgcgcgt ggcgatgcca gtcaatgtta tagaggctga caaacagacc
    57721 gcaaaggccg agggtaatca gccaaatcgc gttaagcggg gagatttgca catcgtagct
    57781 taccagcgac agcgcaccgc tcacgccaac cgcgccagtc agtacagtga agcccgcggc
    57841 tgccgtatac agactaccaa ccgcgccgcc aattccggct atccagccac ttagcgcttt
    57901 ttgaaaagaa aagagaaatg ccagaacagc ggcggcgaca aaccatgcca cgccgctatt
    57961 gatcagggaa attgcgctca tttagcctct ccactttgag cctgctgaaa caaggtgaga
    58021 tcgccaaagt ccgtgttaaa ggtcagctca cgcttacgtt tgctgacgcg ggcgatatcg
    58081 gtgttatcca ccagatgcag ggctttagtc gggcacatcc gcacacaggc cggaccttgt
    58141 tcatcaaagc tacaaaggtc acatttgacg gcgatcgcgc gaatacctgg cacccagtca
    58201 agcaatgtgc tgacacgcgc cggagcaggc ggtgccggtg gcgctttcgg ggtattggcg
    58261 tttgccggaa tatccagcgg acggctgccg gaaaattcaa ttgcgccaaa cgggcaggcg
    58321 atgccgcaca gtttgcagct tacgcacaga ctttcattca actgcacggc cccatcaacg
    58381 cgggtgatgg cgttaaccgg gcagaccacc gcgcagggtg catcttcaca gtggtgacag
    58441 agctgcggcg cagattcttt ttcattgagc atcactctca ggcgcggcat tgattgcagg
    58501 ccgtgctggc gatgcgtctc tgaacaggcg gcctcacagg tgtggcagcc gatacagagc
    58561 gtggagtcgg caattacaaa acgattcacc aggcattcct caggtgattg tcatttttga
    58621 cgaaaacatg ccgatgaaat gtcattttcg acactcatcg acacgcccat ccccaaacag
    58681 gcgtaacgcc tgcaaaacgg gcaaagcctc agctcatgct gccgggcttt gtccctttac
    58741 cagttggctt aaattcaccg gcacattgtt ttcaatggcg gtgtataagc tgtcgtaaac
    58801 gccagcgatt ttttcgagat agtgttccag aacctgttcg cgatcgcgga tgttgacctt
    58861 gccatcaatc atgccgtcaa tgctcagttg cgcctgcgca atccgctgtt tgtcttcgcc
    58921 atcttttgtt tcgacgtaat actcgatggt gtggctgtta aagccatccg ccagggtgac
    58981 gtaaatacga aaatgttcaa aaaggacgaa acagagttct ttgtccggcg cgcagctcat
    59041 ggcgtgatgc aggcgcgctt tcgataacgt gatcccctga accagcgaat tgcagtagat
    59101 gtgccactgg tcctgtagac gacgatgccg ctgtgcgatg taatcggctt tctcgcttat
    59161 ttcccaaata gtcattgtca ggttactcgt ttaacagaga tgccagcttt aagcattttt
    59221 tgcgccaacc tttaatttat tgttattaaa gagattttta agctaaagat gaatttcgtc
    59281 gccgtgtcga cgtgtcattt cgacatcatc gacattattc accgcaggga taatcaacac
    59341 tggcgcaatt attgcttgta gctggcaata gttaatggga ggcgatatgc acgaaataac
    59401 cctctgccaa cgggcactgg aattgatcga acagcaggcc gcaaaacacg gcgcaaaacg
    59461 cgtaactggg gtctggctca aaattggcgc attttcttgt gtcgaaacca gctctcttgc
    59521 cttttgtttt gatctggttt gccgcggcag cgtggcggaa ggttgtaaac tgcacctcga
    59581 agaacaagag gccgaatgct ggtgtgaaac atgccaacag tatgtgacgc tactgaccca
    59641 gcgcgtccgc cgctgtccac agtgtcatgg tgacatgcta cagattgtgg cagacgacgg
    59701 tttacagatt cggcggatag aaatagacca ggagtgagcg atgtgtacaa catgcggttg
    59761 cggtgaaggc aacctgtata tcgagggtga tgaacataac cctcattccg cgtttcgtag
    59821 cgcgccattt gccccggcgg cacgcccgca gatgaaaatc accggcatta aagcgcctga
    59881 atttaccccc agccagactg aagaaggcga cctgcattac ggtcatggcg aagcgggcac
    59941 tcacgcaccg ggcatgagcc agcgtcggat gctggaagtc gaaattgacg tgctggacaa
    60001 aaacaaccgt ctggctgaac gcaaccgcgc gcgctttgct gcccgcaagc aactggtgct
    60061 caacctggtt tccagccctg gttccggtaa aaccaccctg ctgacggaaa ccttaatgcg
    60121 cctgaaagac agcgttccgt gcgcggttat tgaaggcgac cagcaaaccg tgaacgatgc
    60181 cgcacgcatt cgcgctaccg gtacaccagc gattcaggtg aacaccggta aaggctgcca
    60241 tcttgacgca cagatgattg ccgacgccgc accgcgtctg ccactggacg ataacggtat
    60301 tctgtttatc gaaaacgttg gcaacctcgt atgcccggcc agcttcgatc tcggtgaaaa
    60361 acacaaagta gcggtgcttt ccgttaccga aggtgaagac aaaccgctga aatatcggca
    60421 tatgtttgcc gccgcctcgc tgatgctgct caacaaagtt gacctgttgc cgtatctcaa
    60481 ctttgacgtt gagaagtgca tcgcctgcgc ccgcgaagtc aatccagaaa ttgaaatcat
    60541 ccttatttcc gccaccagcg gcgaagggat ggaccagtgg ctgaactggc tggagacaca
    60601 gcgatgtgca taggcgttcc cggccagatc cgcaccattg acggcaacca ggcgaaagtc
    60661 gacgtctgcg gcattcagcg cgatgtcgat ttaacgttag tcggcagctg cgatgaaaac
    60721 ggtcagccgc gcgtgggcca gtgggtactg gttcacgttg gctttgccat gagcgtaatt
    60781 aatgaagccg aagcacgcga cactctcgac gccttacaaa acatgtttga cgttgagccg
    60841 gatgtcggcg cgctgttgta tggcgaggaa aaataatgcg ttttgttgat gaatatcgcg
    60901 cgccggaaca ggtgatgcag ttaattgagc atctgcgcga acgtgcttca catctctctt
    60961 acaccgccga acgccctctg cggattatgg aagtgtgtgg cggtcatacc cacgccattt
    61021 ttaaattcgg cctcgaccag ttactgcctg aaaacgttga gtttatccac ggtccgggtt
    61081 gcccggtgtg cgtactgccg atgggtagaa tcgacacctg cgtggagatt gccagccatc
    61141 cggaagtcat cttctgtacc tttggcgacg ccatgcgcgt accagggaaa cagggatcgc
    61201 tgttgcaggc aaaagcacgc ggtgccgatg tgcgcatcgt ttactcgccg atggatgcgt
    61261 tgaaactggc gcaggagaat ccaacccgca aagtggtgtt cttcggctta ggttttgaaa
    61321 ccaccatgcc gaccaccgcc atcactctgc aacaggcgaa agcgcgtgat gtgcagaatt
    61381 tttacttctt ctgccagcac attacgctta tcccgacgct gcgcagtttg ctggaacagc
    61441 cggataacgg tatcgacgcg ttcctcgcgc cgggccacgt tagtatggtt atcggcactg
    61501 atgcctataa ttttatcgcc agcgattttc atcgtccgct ggtggtcgct ggtttcgaac
    61561 cccttgatct actgcaaggc gtggtcatgc tggtggagca gaaaatagcg gcccacagca
    61621 aggtagagaa tcagtatcgt cgggtggtac cggatgccgg taacctgctg gcgcaacagg
    61681 cgattgccga tgtgttctgt gtcaacggcg acagcgaatg gcgcggctta ggcgtgattg
    61741 aatcttctgg cgtgcacctg acgccggatt atcaacgatt cgatgccgaa gcacatttcc
    61801 gcccggcacc gcagcaggtc tgcgatgacc cgcgcgcgcg ttgtggtgag gtattaacgg
    61861 gcaaatgtaa gccgcatcaa tgcccgctgt ttggtaacac ctgtaatcct caaaccgcat
    61921 ttggtgcact gatggtctcc tccgaaggag cgtgcgccgc gtggtatcag tatcgtcagc
    61981 aggagagtga agcgtgaata atatccaact cgcccacggc agcggcggcc aggcgatgca
    62041 gcaattaatc aacagcctgt ttatggaagc ctttgccaac ccgtggctgg cagagcagga
    62101 agatcaggca cgtcttgatc tggcgcagct ggtagcggaa ggcgaccgtc tggcgttctc
    62161 caccgacagt tacgtcattg acccgctgtt ctttcctggc ggtaatatcg gcaagctggc
    62221 gatttgcggc accgcgaatg acgttgcggt cagtggcgct attccgcgct atctctcctg
    62281 tggctttatc ctcgaagaag gattgccgat ggagacactg aaagccgtag tgaccagcat
    62341 ggcagaaacc gcccgcacgg caggcattgc catcgttact ggcgatacta aagtggtgca
    62401 gcgcggcgcg gcagataaac tgtttatcaa caccgctggc atgggcgcaa ttccggcgaa
    62461 tattcactgg ggcgcacaga cgctaaccgc aggcgatgta ttgctggtta gcggtacact
    62521 cggcgaccac ggggcgacta tccttaacct gcgtgagcag ctggggctgg atggcgaact
    62581 ggtcagcgac tgcgcggtgc tgacgccgct tattcagacg ctgcgtgaca ttcccggcgt
    62641 gaaagcgctg cgtgatgcca cccgtggtgg tgtaaacgcg gtggttcatg agttcgcggc
    62701 agcctgcggt tgtggtattg aaatttctga atcagcgctg ccggttaaac ctgccgtgcg
    62761 cggcgtttgc gaattgctgg gactggacgc cctgaacttt gccaacgaag gcaaactggt
    62821 gatcgccgtt gaacgcaacg cggcagagca agtgctggca gcgttacatt cccatccact
    62881 ggggaaagac gcggcgctga ttggtgaagt ggtggaacgt aaaggtgttc gtcttgccgg
    62941 tctgtatggc gtgaaacgaa ccctcgattt accacacgcc gaaccgcttc cgcgtatatg
    63001 ctaataaaat tctaaatctc ctatagttag tcaatgacct tttgcaccgc tttgcggtgc
    63061 tttcctggaa gaacaaaatg tcatatacac cgatgagtga tctcggacag caagggttgt
    63121 tcgacatcac tcggacacta ttgcagcagc ccgatctggc ctcgctgtgt gaggctcttt
    63181 cgcaactggt aaagcgttct gcgctcgccg acaacgcggc tattgtgttg tggcaagcgc
    63241 agactcaacg tgcgtcttat tacgcgtcgc gtgaaaaaga cacccccatt aaatatgaag
    63301 acgaaactgt tctggcacac ggtccggtac gcagcatttt gtcgcgccct gatacgctgc
    63361 attgcagtta cgaagagttt tgtgaaacct ggccgcaggt gggctatacc ccaaatttgg
    63421 tcactattgc ctgatgccac tggcggcgga agggcatatt tttggtggct gtgaatttat
    63481 tcgttatgac gatcgtccct ggagcgaaaa agagttcaat cgtctgcaaa catttacgca
    63541 gatcgtttct gtcgtcaccg aacaaatcca gagccgcgtc gtcaacaatg tcgactatga
    63601 gttgttatgc cgggagctcg ataacttccg catcctggtt gccattacca acgcggtgct
    63661 ttcccgcctg gatatggacg aactggtcag cgaagtcgcc aaagaaatcc attactattt
    63721 cgacattgac gatatcagca ttgtcttacg cagccaccgt aaaaacaaac tcaacatcta
    63781 ctccactcac tatcttgata aacaacatcc cgcccacgaa cagagcgaag tcgatgaagc
    63841 cggaaccctc accgaacgcg tgttcaaaag taaagagatg ctgctgatca atctccacga
    63901 gcgggacgat ttagccccct atgaacgcat gttgttcgac acctggggca accagattca
    63961 aaccttgtgc ctgttaccgc tgatgtctgg cgacaccatg ctgggcgtgc tgaaactggc
    64021 gcaatgcgaa gagaaagtgt ttaccactac caatctgaat ttgctgcgcc agattgccga
    64081 acgtgtggca atcgctgtcg ataacgccct cgcctatcag gaaatccatc gtctgaaaga
    64141 acggctggtt gatgaaaacc tcgccctgac cgagcagctc aacaatgttg atagtgaatt
    64201 tggcgagatt attggccgca gcgaagccat gtacagcgtg cttaaacaag ttgaaatggt
    64261 ggcgcaaagt gacagtaccg tgctgatcct cggtgaaact ggcacgggta aagagctgat
    64321 tgcccgtgcg atccataatc tcagtgggcg taataatcgc cgcatggtca aaatgaactg
    64381 cgcggcgatg cctgccggat tgctggaaag cgatctgttt ggtcatgagc gtggggcttt
    64441 taccggtgcc agcgcccagc gcattggtcg ttttgaactg gcggataaaa gctctctgtt
    64501 cctcgacgaa gtgggcgata tgccactgga gttacagccg aagttgctgc gtgtattgca
    64561 ggaacaggag tttgaacgtc tcggcagcaa caaaatcatt cagacggacg tacgtttaat
    64621 tgccgcgact aaccgcgatc tgaaaaaaat ggtcgccgac cgagagttcc gtagcgatct
    64681 ctattaccgc ctgaacgtat tcccgattca cctgccgcca ctacgcgagc gtccggaaga
    64741 tattccgctg ctggcgaaag cctttacctt caaaattgcc cgtcgtctgg ggcgcaatat
    64801 cgacagcatt cctgccgaga cgctgcgcac cttgagcaac atggagtggc cgggtaacgt
    64861 acgcgaactg gaaaacgtca ttgagcgcgc agtattgcta acacgcggca acgtgctgca
    64921 gctgtcattg ccagatattg ctttaccgga gcctgaaacg ccgcctgccg caacggttgt
    64981 cgcccaggag ggcgaagatg aatatcagtt gattgtgcgc gtgctgaaag aaaccaacag
    65041 cgtggttgcc gggcctaaag gcgctgcgca acgtctgggg ctgaaacgca cgaccctgct
    65101 gtcacggatg aagcggctgg gaattgataa atcggcattg atttaactgc aaattgccgg
    65161 acagatctgc ctgtccggca tactattcac gaggtttttt cggacgatat ttttccggca
    65221 gttctggcac cggacgcttg tcatcgatga gatgacgcac ggttaagatc ggatgacgcc
    65281 acagcattct cggcccggcc caacgcataa tctgtttcat ctcttcacgc tttgcaggct
    65341 ggtaacagtg caccggacac tgcttacagg ctggtttctc ttcgccgaac acacatttat
    65401 ccagccgctt ttgcgcgtaa acaaacaacg cctcgtaatg ctccggctcc gctgacgcct
    65461 gcgggcattt cgcttgataa agatcgatca tttttttaat cgtcagtttt tcacgagaga
    65521 tacgcttgcc ggacatgctg cctccacctc attaagatgc atttatatta catcttaatc
    65581 ttaaagggca ctatgactct aaagaagaag ggttcgccaa tcgatacaat tttgcgtact
    65641 tgcttcataa gcatcacgca aaagctgcaa aacagcatct ttcccggaac cagcatcaag
    65701 aactcgccgt tcgcttcttc ccctgaaatg attaactccg gtatcatgtg cgccttatgt
    65761 gattacaacg aaaataaaaa ccatcacacc ccatttaata tcagggaacc ggacataacc
    65821 ccatgagtgc aatagaaaat ttcgacgccc atacgcccat gatgcagcag tatctcaagc
    65881 tgaaagccca gcatcccgag atcctgctgt tttaccggat gggtgatttt tatgaactgt
    65941 tttatgacga cgcaaaacgc gcgtcgcaac tgctggatat ttcactgacc aaacgcggtg
    66001 cttcggcggg agagccgatc ccgatggcgg ggattcccta ccatgcggtg gaaaactatc
    66061 tcgccaaact ggtgaatcag ggagagtccg ttgccatctg cgaacaaatt ggcgatccgg
    66121 cgaccagcaa aggtccggtt gagcgcaaag ttgtgcgtat cgttacgcca ggcaccatca
    66181 gcgatgaagc cctgttacag gagcgtcagg acaacctgct ggcggctatc tggcaggaca
    66241 gcaaaggttt cggctacgcg acgctggata tcagctccgg gcgttttcgc ctgagcgaac
    66301 cggccgaccg cgaaacgatg gcggcagagc tgcaacgcac taatccggcg gaactgctgt
    66361 atgcagaaga tttcgccgag atgtcgctga ttgaaggccg tcgcggcctg cgccgtcgcc
    66421 cgctgtggga gtttgaaatc gacaccgcgc gccagcagtt gaatctgcaa tttggcaccc
    66481 gcgatctggt cggttttggc gtcgagaacg cgccgcgcgg actttgtgct gccggttgtc
    66541 tgttgcagta tgcgaaagat acccaacgca cgaccctgcc gcatattcgt tctatcacca
    66601 tggaacgtga gcaggacagc atcattatgg atgccgcgac acgtcgtaac ctggaaatta
    66661 ctcagaacct ggcgggcggt gcggaaaata cgctggcttc tgtgctcgac tgcaccgtca
    66721 cgccgatggg tagtcgtatg ctgaaacgct ggctgcatat gccagtgcgc gatacccgcg
    66781 tgttgcttga gcgccagcaa actattggcg cattgcagga tttcaccgcc gagttgcagc
    66841 cggtactgcg tcaggtcggc gacctggaac gtattctggc acgtctggct ttacgaactg
    66901 ctcgcccacg cgatctggcc cgtatgcgcc acgctttcca gcaactgccg gagctgcgtg
    66961 cgcagttaga aactgtcgat agtgcaccgg tacaggcgct acgtgagaag atgggcgagt
    67021 ttgccgagct gcgcgatctg ctggagcgag caatcatcga cacaccgccg gtgctggtac
    67081 gcgacggtgg tgttatcgca tcgggctata acgaagagct ggatgagtgg cgcgcgctgg
    67141 ctgacggcgc gaccgattat ctggagcgtc tggaagtccg cgagcgtgaa cgtaccggcc
    67201 tggacacgct gaaagttggc tttaatgcgg tgcacggcta ctacattcaa atcagccgtg
    67261 ggcaaagcca tctggcaccc atcaactaca tgcgtcgcca gacgctgaaa aacgccgagc
    67321 gctacatcat tccagagcta aaagagtacg aagataaagt tctcacctca aaaggcaaag
    67381 cactggcact ggaaaaacag ctttatgaag agctgttcga cctgctgttg ccgcatctgg
    67441 aagcgttgca acagagcgcg agcgcgctgg cggaactcga cgtgctggtt aacctggcgg
    67501 aacgggccta taccctgaac tacacctgcc cgaccttcat tgataaaccg ggcattcgca
    67561 ttaccgaagg tcgccatccg gtagttgaac aagtactgaa tgggccattt atcgctaacc
    67621 cgctgaatct gtcgccgcag cgccgtatgt tgattattac cggcccgaac atgggcggta
    67681 aaagtaccta tatgcgccag accgcactga ttgcgctgat ggcctacatc ggcagctatg
    67741 taccggcaca aaaagtcgag attggaccta tcgatcgcat ctttacccgc gtaggcgcgg
    67801 cagatgacct ggcttccggg cgctcaacct ttatggtgga gatgactgaa accgccaata
    67861 ttttacataa cgccaccgaa tacagtctgg tgttaatgga tgagatcggg cgtggaacgt
    67921 ccacctacga tggtctgtcg ctggcgtggg cgtgcgcaga aaatctggcg aataagatta
    67981 aggcattgac gctgtttgcc acccactatt tcgagctgac ccagctaccg gagaaaatgg
    68041 aaggcgtcgc caacgtacat ctcgatgcgc tggagcacgg cgacactatc gcctttatgc
    68101 acagcgtgca ggacggcgcg gcgagcaaaa gctacggtct ggcggttgct gctctggcgg
    68161 gcgtaccgaa agaggttatt aagcgcgcac ggcaaaaact gcgtgagctg gaaagcattt
    68221 cgccgaacgc cgccgcgacg caagtggatg gtacgcagat gtctttgctg tcagtgccag
    68281 aagaaacttc gcctgcggtc gaagcactgg aaaatctcga cccggattca ctgactccgc
    68341 gtcaggcgct ggagtggatt tatcgcttga agagtctggt gtaataacaa ttcccgatag
    68401 tcttttgcta tcgggaatat taacgacaac tgacgaatca aataaaaata ccctgtataa
    68461 taggaaagct tattttacag ggtaaaacca tgccatctac acgctatcaa aaaatcaatg
    68521 cccatcacta tcgccatata tgggtcgttg gtgatattca tggtgaatat cagttattac
    68581 aatcccgctt acatcaactc tcttttttcc ccgaaaccga cttacttatt tctgtcggcg
    68641 ataatattga tcgtgggccg gagagtcttg acgtcctgcg cctgctaaac cagccctggt
    68701 ttatctccgt taaaggcaac cacgaagcaa tggcgctgga tgcgttcgcg accggcgatg
    68761 gcaatatgtg gcttgccagc ggtggtgact ggtttttcga tttaaatgat tcagagcaac
    68821 aagaggcaat agatctgttg ctgaaattcc atcaccttcc acatattatt gaaatcacta
    68881 acgacataat aaaatatgtc atcgcacatg cagattatcc gggtgatgaa tatctctttg
    68941 gcaaagaaat agcggagagc gaattactct ggcctgttta tcgtatgcag aaatcgctta
    69001 atggcgagtt acaacaaata aacggcgctg attattttat atttggacat atgatgtttg
    69061 ataacattca gacgttcgct aaccagattt atattgatac cggatcgccg aaaagtgggc
    69121 ggctgtcatt ttataaaata aggtagtctc atgcttcttc tgtgaagcat gagtaaccag
    69181 atgttattgc aggccattat tcatttttcg ctaccagcaa agagagatcc tgcttcgcca
    69241 gcgcgcgact ggcactctcc ggcaaaccgt cgtctgtaat aatctgatca aactcgctta
    69301 atggtaacgc cagccatgtc gccacctgac catatttcgt cgcatcacag accaaaactc
    69361 gctggcggct ggcactggca atcgcccgtt tcaccgtgac tttatcttcc gctggcgtag
    69421 aaatcccccg cacactccat gacgatgcag aaataaaagc ctgatcaatc atcaggctgc
    69481 gcagcatggt cgcagcggct tccccgacac aggaacggtt ttcccgacac actgcaccgc
    69541 cagtgtgaat aattgtgcaa ttactgttgt cgagcaagta gttcgcaata acgaaatcgt
    69601 ttgtgaccac agtcagtgac tccatgtgaa tcagatgctg tgctatcgct aacgtggtcg
    69661 ttcccgcatc cagatagata caacttcccg gctgaacaag acttgccgcc agcttgccaa
    69721 tagccgcttt ttgcgtcatt gccagcgcag tttttacctg atgagaaggt tcatgcgcca
    69781 cgcgtcccgg agactggacg cctccggaca ccagcacaac ggctccctgc tgctccagtt
    69841 tttgtaaatc ccgacgaatg gtcatatgtg acacattaat tctgtccgtt agttcagcaa
    69901 tactgacaat gcctttttca gctaccatct caaggatgat ttggcgacgc tctacgggta
    69961 tcaacttttg ctccttcctt tgtcctgctg acattctacg ctatttgcct gcgaaacgtg
    70021 cgcggcgcaa ctaacgctta gttcacataa aataacacaa aatgttaatt tatgtgaatc
    70081 agctcaccat atcgttatct ttcagcgctc atattcacaa tatcaaacaa aatatcactt
    70141 aaattaacaa ggagagcaga tgaaaacggg atctgagttt catgtcggta tcgttggctt
    70201 agggtcaatg ggaatgggag cagcactgtc atgtgtccgc gcaggttttt ctacctgggg
    70261 cgcagacctg aacagcaatg cctgcgctac gttgaaagag gcaggtgctt gcggggtttc
    70321 tgataacgcc gcgacgtttg ccgaaaaact ggacgctctg ctggtgctgg tggtcaatgc
    70381 ggcccaggtt aaacaggtgc tgtttggtga aacaggcgtt gcacaacatc tgaaacccgg
    70441 tacggcagta atggtttctt ccactatcgc tagtgctgat gcgcaagaaa ttgctaccgc
    70501 tctggctgga ttcgatctgg aaatgctgga tgcgccagtt tctggtggtg cagtaaaagc
    70561 cgctaacggt gaaatgactg tcatggcctc cggtagcgat attgcctttg aacgactggc
    70621 acccgtgcgg ggatcccgct ggatgtgatg tatgacgtcg tgaccaatgc cgccggaaat
    70681 tcctggatgt tcgaaaaccg gatgcgtcat gtggtggatg gcgattacac cccgcattca
    70741 gccgtcgata tttttgttaa ggatcttggt ctggttgccg atacagccaa agccctgcac
    70801 ttcccgctgc cattggcctc aacagcattg aatatgttca ccagcgccag taacgcgggt
    70861 tacgggaaag aagacgatag cgcagttatc aagattttct ctggcatcac tctaccggga
    70921 gcgaaatcat gatcaagatt ggcgttatcg ccgatgattt taccggcgcg acggatatcg
    70981 ccagttttct ggtggaaaac ggtctaccaa cggtacaaat taacggtgtt ccaacaggta
    71041 aaatgccgga agcaatcgac gcactggtga tcagcctgaa aacgcgctcc tgtccggtgg
    71101 ttgaagccac acagcaatcg ctggcggctc tgagctggtt gcaacagcaa ggttgcaaac
    71161 agatctattt caaatactgc tctactttcg acagtacggc gaaaggtaat attggcccgg
    71221 ttaccgatgc attaatggat gctctcgaca cgccgtttac ggtcttctct ccggccctgc
    71281 cggtcaacgg acgtacggtt tatcaggggt atttgttcgt aatgaatcaa ctgctggccg
    71341 aatccgggat gcaccatcac ccggtaaatc ccatgaccga ccgctatctt ccccgtctgg
    71401 ttgaagcgca atccacaggg cgctgcggcg tcgtttcggc acatgttttc gaacaaggtg
    71461 tggatgccgt tcgtcaagag ctggctcgct tacagcaaga gggctaccgc tacgcggtgc
    71521 ttgatgcgct gaccgaacac catctggaaa ttcagggaga agccttgcgc gatgccccac
    71581 tggtaacggg cggttctggt ctggcgattg gcctggcccg gcagtgggcg caagaaaacg
    71641 gtaaccaggc tcgcgaagca gggcgtccgc tcgctgggcg cggcgtagtg ctctccggtt
    71701 catgctctca aatgaccaac cgccaggtgg cacattaccg tcaaattgca ccagcccgtg
    71761 aagttgatgt ggcacgctgc ctctcaaccg aaactctggc cgcttatgca cacgaactgg
    71821 cagagtgggt tctgggccag gaaagtgtac ttgctccact ggtttttgcc accgccagca
    71881 ctgacgcatt ggcagcaatt caacagcaat acggtgcaca aaaagccagt caggcagtag
    71941 aaactctgtt ttctcaacta gcggcgcggt tagcagcgga aggcgtgaca cgctttattg
    72001 tcgcaggcgg tgagacctcc ggcgtagtca cacagagcct gggcataaaa gggtttcata
    72061 ttggcccaac catttccccc ggcgtgccgt gggtaaacgc actggataag cctgtctcac
    72121 tcgcccttaa atctggcaac ttcggtgatg aagccttttt ttcacgagcc caaagagagt
    72181 ttttatcatg agcgatttcg caaaagtaga gcagtctttg cgagaggaga tgacgcggat
    72241 tgccagttca ttctttcagc gcggctatgc aaccggttcg gctggcaatc tgtcgctgct
    72301 tttacctgac gggaatttac tggcgacacc gacaggttca tgcctgggca atctcgatcc
    72361 gcagcggctt tccaaagtcg ccgcggatgg cgaatggtta agtggtgaca aaccctcgaa
    72421 agaggtgctc tttcatctgg cgctgtatcg caacaatccg cgctgtaaag cggtggtgca
    72481 tttgcacagc acatggtcga cggcgctttc ctgcctgcaa gggctggaca gcagcaacgt
    72541 tattcgtccg ttcacaccat acgtggtgat gcggatggga aatgtcccgc tggtgcccta
    72601 ttaccgaccg ggcgataaac gcatcgcaca ggatctggcg gaactggcag cagacaatca
    72661 ggctttttta ctggcaaatc atggcccagt ggtttgcggt gaaagcctgc aagaagccgc
    72721 caacaatatg gaagagctgg aggaaacggc aaagctgatt tttattctcg gtgaccgccc
    72781 gatccgttat ctgaccgcag gtgaaattgc ggaattaagg agttaatgca atgcctcgtt
    72841 ttgcagctaa tttatccatg atgttcaccg aagtgccttt tattgaacgc ttcgccgcag
    72901 cgcgaaaagc cgggttcgat gctgtggaat ttctgtttcc ctataactac tccaccctgc
    72961 aaatccaaaa gcaactggag caaaaccatc tgacactggc gctgttcaat accgctcccg
    73021 gagatattaa tgcgggggaa tggggattat ccgcccttcc cggacgtgag cacgaagcac
    73081 acgcagacat cgacctggca ctggaatatg cattggcgct caattgtgaa caagtccatg
    73141 tgatggcagg cgtcgtgccc gctggcgaag atgctgaacg gtaccgggca gtatttatcg
    73201 ataatctccg ctacgctgcc gaccgctttg cgccacacgg caagcgaata ttagttgaag
    73261 cactcagccc cggcgttaag cctcattatc tcttctccag ccagtatcag gcactggcta
    73321 ttgttgaaga ggttgcgcga gataacgtgt ttattcaact agatactttt catgcgcaaa
    73381 aagtagatgg caacctgacg catttaatcc gcgactacgc cgggaaatat gcgcacgtac
    73441 aaattgccgg actacctgat cgacatgaac cggacgatgg agaaatcaac tatccgtggc
    73501 tgttccgcct gttcgatgag gtgggatatc agggctggat cggttgtgaa tataaacctc
    73561 gtggcctcac cgaagaaggg cttggctggt ttgacgccta gcgctaattc gttaaatccc
    73621 taattacaac gtacccatac atccccccat ctacggatgg ggtaggttaa ctataacttt
    73681 cagacagggt ttcccatgtc cacaattaca ttgttatgca ttgcgttagc tggcgtaatc
    73741 atgctgttgc tgctggtcat caaggcaaag gtacaaccat tcgttgctct gctcctcgtc
    73801 agcctgttag tcgcacttgc ggcgggtata ccggcgggcg aagtgggtaa agtgatgatc
    73861 gccgggatgg gcggtgtgct tggttccgtc actattatta ttggtctggg cgctatgctg
    73921 gggcgtatga tcgaacactc ttgtggtgca gagtcactgg ctaattattt cagtcgcaag
    73981 ttaggtgaca aacgaactat cgctgcgctg actctggcag cgttcttcct cggtattccc
    74041 gtcttctttg atgtcggctt tattattctt gcgccaatca tttacggttt tgccaaggta
    74101 gccaaaatat cgccactcaa atttggcctg cctgtcgctg ggatcatgct cactgttcac
    74161 gtggcggtac cgccgcatcc aggccctgtc gccgcagcgg ggttactcca cgcagacatc
    74221 ggctggctaa ccatcatcgg tattgcgatt tctattcccg taggggttgt tggctacttt
    74281 gcagcgaaaa taatcaataa acgccaatat gcgatgtcag tagaagtact ggaacagatg
    74341 caactggctc cggccagtga ggaaggcgcg acaaaattaa gcgataaaat aaatccaccg
    74401 ggcgtcgcgc tggtcacctc gctaattgtt attcctatcg cgattatcat ggtgggtacg
    74461 gtttccgcaa cactgatgcc gccttcgcat cccctgcttg gtacgctaca gctgatcggc
    74521 tcaccaatgg tagctctaat gattgcgctg gtgctggcat tctggttatt ggctttacgt
    74581 cgcggctgga gcttacaaca caccagcgac attatgggct cagcgcttcc tactgcggcg
    74641 gtagtgattt tggttactgg tgctggaggg gtatttggca aagtgctggt ggaatcgggc
    74701 gtcggcaaag cccttgccaa catgttacaa atgattgacc tgcctctgtt accagccgca
    74761 tttattattt cactggcgct gcgtgcatcg caggggtcag caaccgtagc aatactgaca
    74821 accggcgggt tactctcaga agcggtgatg ggattgaatc cgattcagtg cgtattggtg
    74881 acgctggcag cctgctttgg tgggcttggg gcgtcacata ttaatgactc agggttctgg
    74941 attgtgacca aatatctggg gttgtcggta gcagacggtc tgaaaacctg gacagtgtta
    75001 acgaccattc tcggttttac cggattctta attacctggt gcgtatgggc ggtaatttga
    75061 ccttaataaa aaggtccgat gggcatcgga cctttttatt tagcgcttac cttccgccag
    75121 cagcggcggg atgctcggca ccattggcgc atcatcaata tctttctgcg tcatgcggaa
    75181 cgcttccggg taatgttcgc gactggtgcg gcgtagcggt tcggtatcgc gccaggtata
    75241 aaggcaatgc tggcactgat ataccgtcca gacatctttc accggcgatt tcaccatcac
    75301 ttcaatctgt tcatcggcac aacgtggaca aatcatcttg ttctccttat ttacgtgcag
    75361 ccagcatagt ggtcagtttt tcagcccagg ctttggtttc cggcaaatcc accaccggct
    75421 ggctgtagtg accacggttg tccggggcga caggcgtggt agcgtcgata atcagcttgt
    75481 cggtgatccc cgccgggctt gagccagggt cgagttccag cacggacata ttcggcaact
    75541 gcaccaaatc ccctgccggg tttactttcg aggagagcgc ccacatcacc tgcggcaggt
    75601 tgaacgggtc aacgtcttca tcgaccataa tcaccatctt tacgtagccc agaccgtgcg
    75661 gcgtggtcat cgcacgcagg cccaccgcgc gggcaaagcc gccgtagcgt tttttggtgg
    75721 agataatcgc cagcaggccg tgggtgtaca tggcatttac cgcctgcact tccgggaact
    75781 cggctttcag ttgttgatac agcggtacgc aagtggctgg ccccatcagg tagtcgattt
    75841 cggtccacgg cataccgaga tagagtgatt cgaaaatcgg tttgctgcga taagagactt
    75901 tatcgatgcg caccacggtc atgttgcgcc caccggagta gtgcccggta aactcaccga
    75961 acggcccttc gatttcgcgt ttacggcttt cgataacccc ttcgaggatc acttctgaac
    76021 cccacggcac atcaaaaccg gtcagcgggg cggtggcgat cgggtacggg ctttcacgca
    76081 gcgcgcctgc catttcatac tcagactgat cgtatttcag cggcgtggcc cccatcagcg
    76141 tgatgatcgg atcgttaccg agcgtgatcg caatcggcag atcttcaccg cgctcttctg
    76201 ctttatgcag atgcagggcg atatcgtgca tcggcaccgg ttgcaggccg agcttacgct
    76261 tgcccttcac ttccatgcgg tagatgccga cgttctgctt gccgaagtta tccgggtcga
    76321 gcggatcgcg ggaaaccacg cacgctttgt cgagatagaa accgccatcg ccatcattca
    76381 ggcgaaacag cggcagaata tcgaacaggt tgatctcttc gccatcgacg gtgttctgcg
    76441 cccaggctgg attggcgcgg cgctccgggg caatcgggaa gttatcccag cggcggataa
    76501 actcatcaat ctgctttttg accggggtgt ttggtggcag gccaagggaa atcgcgtggt
    76561 tctgccagga accgatggta ttcatcgcca cgcgggcatc ggtaaagccg cgaatattat
    76621 caaaccacag cgcgggcgcg ccgtcgccga tacgcccggt ggcgttagct gctgctgcca
    76681 gatccggctc agcgttcact tcttcgctga ttttcagtaa ctggccgtgg tcatcaagcg
    76741 cctgtaaaaa gctgcgtaaa tcatcaaatg ccattattca ttctcctgag aaaaattccg
    76801 ggcctgcggc aatccttgcc agcgcctggc gtggggatgt tcgaggccaa attgatccag
    76861 cacgcgggct accacatggt ggacaatgtc atctaccgtt tcgggatggt tataaaaggc
    76921 aggcatcggc ggcaccatcg ccacgcccat gcgtgaaagt gcgagcatat tttcgagatg
    76981 gatggtgcta agcggcattt cacgcggcac cagcaccagt ttgcggcctt ctttgagcac
    77041 gacgtccgcc gcgcgcccta ccaggccatc ggcgtaacca gcgcggatac cggcgagcgt
    77101 tttcatactg cacggaataa cgatcatgcc gtcggtacga aaagaaccgg atgagatgat
    77161 cgccgcctga tccgccgggt tatggctgaa gtctgcgagg gcagcaacat cgcgagcgct
    77221 gtaaggcgtt tccagttcaa tggtggtttt cgcccacttc gacatcacca gatgagtctc
    77281 gacattcggc atctcacgca gcgcttgcag taatgccaca ccaagaggcg caccggtagc
    77341 ccctgtcatc ccgacgatca gtttcattgc ttattctctt aattgttcgc atacgaacgt
    77401 tattattaac gtactccttc atttccacag cggcaaggat tgaaaagtaa caacatcttc
    77461 ttcgtcacta aaaaaccgct atgatagcga cagagaatta gcccggagtt tacatggcgt
    77521 tacgaaataa agcgttccat cagttacgac agctttttca gcagcacacg gctcgctggc
    77581 agcacgagtt acctgacctg accaaaccac agtacgcggt gatgcgcgcc attgctgata
    77641 aacctggtat tgaacaggtg gcactgatag aagcagcagt cagcaccaaa gcaacgctag
    77701 cagaaatgtt ggcaagaatg gaaaatcgtg gcttagtcag acgtgaacat gacgtcgctg
    77761 acaagcggcg acgctttgtc tggctgactg ccgaaggaga aaagctactt gctgcggcga
    77821 tacctattgg tgacagcgtg gatgaggaat ttttggggcg tttgagtgga gaagagcagg
    77881 agctgtttat gcagttggtg cgcaagatga tgagcacata gaatgctgat tgccgggtag
    77941 gacgctgacg tgtcttatcc aggcgacaaa aacacaagct tcccaaacaa aagaaaaagg
    78001 ccagcctcgc ttgagactgg cctttctgac agatgcttac ttactcgcgg aacagcgctt
    78061 cgatattcag cccctgcgtt tgcaggattt cgcgcaaacg gcgcaggcct tcaacctgaa
    78121 tctggcgaac acgttcacgg gtgaggccaa tttcacgacc tacatcttcc agtgttgccg
    78181 cttcgtaccc cagcaaaccg aatcgacgtg ccagtacttc acgctgtttg gcgttcagct
    78241 cgaacagcca tttgacgatg ctctgcttca tatcgtcatc ttgcgtggta tcttccggac
    78301 cgttctcttt ttcatcggcc aggatgtcca gcaacgcttt ttcggaatca ccacccagcg
    78361 gggtgtctac cgaggtaatg cgctcgttaa gacgaagcat acagctgacg tcatcaactg
    78421 gcttatccag ttgctctgcg atctcttccg cacttggttc atggtccagc ttatgggaca
    78481 actcacgtgc ggttcgcagg taaacgttca gctcctttac gatgtgaatc ggcaaacgaa
    78541 tagtacgggt ttggttcata atcgcccgtt caatcgtctg gcgaatccac caggttgcgt
    78601 atgttgagaa gcggaaacca cgttccgggt caaacttctc taccgcgcgg atcagcccca
    78661 agttgccctc ttcgataagg tccagcaacg ccagaccacg attgccataa cggcgggcaa
    78721 tttttaccac cagacgcaag ttactctcga tcatccggcg gcgagaggcg acatctccac
    78781 gcagtgcgcg acgcgcaaaa taaacttctt cttcggccgt taacagtggt gaataaccaa
    78841 tctcaccaag gtaaagctga gtcgcgtcca acacacgctg tgtggctccc tgcgataaca
    78901 gttcctcttc ggccaaatcg ttatcactgg gttcctcttc tactaaggcc ttttcgtcaa
    78961 aaacctcaac tccgttctca tcaaattccg catcttcatt taaatcatga actttcagcg
    79021 tattctgact cataaggtgg ctcctacccg tgatcccttg acggaacatt caagcaaaag
    79081 cctggttccg ccgatttatc gctgcggcaa ataacgcagc gggtttacgg atttcccctt
    79141 gtaacgaatt tcaaaatgca agcgtgttga actggttccg gtgctaccca tggttgctat
    79201 tttttgcccc gccttcactt cttgttgttc ccggaccagc attgtgtcgt tatgggcgta
    79261 ggcactcagg taatcatcat tatgtttgat gataatcaga ttaccgtagc cgcgcagcgc
    79321 gttaccagca taaacaacgc ggccatctgc ggtcgcgata attgcctgtc ctttgctgcc
    79381 tgcgatatca atccccttgt tgcccccctc agaagcgcca aaggtttcga tcactttgcc
    79441 ctcagtcggc cagcgccagg tggagatagg cgtactggtt gatgtactgc tgacagtcgg
    79501 ctcggttgtg cttgctgttg gtaccgttac aggcgctgtg accgtggtcg cagttggctt
    79561 gttgttcggc aacattttgt tagcactctg ttcacccgaa gactcagaat acgtaattgt
    79621 cggttgcgac gcaacagcaa cggtggaatt ttgtgcaggc ttgatcacaa ctccttgctc
    79681 tgctgcgtcg gcctgggtaa tggcatttcc gccagtgatt ggcgtaccgg aagcattacc
    79741 cacctgcaag gtctgaccaa cgttcagcgc gtatggtgcc tgaatattgt tgcgctgagc
    79801 aaggtcacgg aaatcgttgc cagtaatcca ggcgatatag aaaagtgtgt cgcctttttt
    79861 cacggtatag gtactgccgc tataactgcc tttcggaatg ttcccatact gacggttata
    79921 gacgatgcgt ccgttttcca tctgtaccgg ctgctgagct actggctgca ctggctggat
    79981 ttgcggttgt tgagtagcct gaatttgtgg ctgctgtacc ggctgaattt gcggttgctg
    80041 cgctgtagac gtcgtcccca ttttcggcgg cggcgtaatc aacataccag aattagtatt
    80101 tgcaggcgca ttgccattaa cggagctgac cggtgccggt ggatttgaag tgtcagaaca
    80161 gcctgccagc catagcgaaa ccagtgacaa agccgcaatg cggcgaacgg tgaattttgg
    80221 gcttcccgcg ctcatttatc ccccaggaaa aattggttaa taaccagtga cataattacc
    80281 gtgcaaggca ccctactgaa cactggaaaa gatgttcacg atacgctgac ctacggcaaa
    80341 ataaccagga aaaatccagg tatttcctca cgttttaagc cagctcaccc ttcactaaag
    80401 ggacaaagcg cacggcctcc acggtatcga taataaattc gcctccccga cggcgcaccc
    80461 gtttcaaata ctggtgctcc tcccctacgg gtaagacgag aatcccgcct tcgtccagct
    80521 gcgtcattag cgcagttgga atttccggcg gtgccgccgt aacaatgata gcgtcaaacg
    80581 gcgcacgtgc ctgccaacct tgccatccat cgccatgacg ggttgaaaca ttatgtaaat
    80641 caagattttt caggcggcga cgtgcctgcc actgcaagcc tttaatccgt tcaaccgagc
    80701 aaacatgctg gacaagatgc gccaggattg ccgtttgata tcccgaacca gtgccaattt
    80761 ccagcacccg cgactgcggc gtcagctcga gtaattcggt cattcgcgcc accatatacg
    80821 gctgcgaaat tgtctgcccc tgacctatcg gcaaggcgat attgtcccag gctttttgtt
    80881 caaacgcttc atcaacgaat ttttcacgcg gcacggcggc aagtgcattc agcacctgct
    80941 catcctgaat accttgcgca cgtaattgat ccagaagtgc ttgtacgcgt ctgcttacca
    81001 ttgcgtgcca actcccacgc tgtttaacca gtctgaaacc acatcttgcg cgctatgcgc
    81061 agttaaatcc acatgcagcg gcgtgatgga gacatagccc tcatctaccg cagcaaaatc
    81121 ggtccccgga ccggcatcac atttaccgcc cggcgggcca atccagtaca gcgtattgcc
    81181 gcgcggatct tgctgcggga tcacttgatc tgccggatgt cgtgtaccgc agcgcgtcac
    81241 gcgaatacct ttgatttgat ccaagggtaa atccggaacg ttaatattaa gaatacgtcc
    81301 ggtgcgcagc ggctctttac acagtgcgcg caaaattgaa caggttaccg ccgcggcagt
    81361 gtcgtaatgt ttatgcccgt caagcgagac ggccagcgcc ggaaaaccta aatgacggcc
    81421 ttccatcgcg gcggctaccg taccggaata aataacatca tcccccagat tcggcccggc
    81481 gttaattccg gacacaacaa tgtccgggcg cggacgcatc agagcattca cgccaagata
    81541 gacacaatcg gtcggggttc ccatttgcac agcaatatca ccattttcaa aggtaaacgt
    81601 gcgcagggag gattccagtg tcagagaatt tgaagcgccg ctgcggttac gatcgggggc
    81661 gaccacctga acgtcagcaa actcacgcaa ggctttcgcc agcgtttgta taccgggtgc
    81721 atgtaccccg tcatcattac tcagcaatat gcgcataatc acctgttgtg ttgataagtt
    81781 ccctgacaac gctggttgca aaactacccg ccggaagcca gaaacggatc tctacggtga
    81841 cgtcatccca ccagttccag cttaattgtt gcggatacag cagcatcgct ctgcgcgcgg
    81901 cttcaacttt ttcgcgcacc agtaaagctt gtaattcagt ttctgcggcg acagctgctt
    81961 gttcgaatgc cagcgcttca cgctgagttc cccattcgcc actgcccggc aatgcggcgg
    82021 ttatcatcag ctctttatcg ttgacgcgac gctgtaattc cgccagttct tcggtggttg
    82081 ctacaaacca gctaccacgt ccggctaatt gtagcgcatc gccgtcaaca acttgattaa
    82141 cgtctgcttt tttgaggcgc tcagcaacaa tctgattaaa caacgcactg cgggctgccg
    82201 acaaccagaa actccgttta ttgcgatcgc gcaccggagt attggtttgc gcccagcgca
    82261 gcgcgccctg caagttgcta ccgccaatcc caaaacgttg ggcaccgaag tagttcggta
    82321 caccttttac gcaaatatcg atcagacgtt gttcaacgtc atcgcgattg ctcacttcgc
    82381 gcagaaccag ggtaaaggcg ttacctttca gcgcccctaa acgcagcttg cgcttgtgcc
    82441 gcgcatactc cagcacctgg cagccttcca gttgaaaggc gctcagatcg ggcatttcct
    82501 tgcccggcac gcgagcacat aaccactgtt ccgttacagc atgtttgtct ttttgcccag
    82561 cgaagctgac ttcacgggaa tgaattttca ggaatttcgc cagtgcatcc gccacaaaac
    82621 gggtattgca gccgtttttg aggattctaa ccagaatatg ctcaccttca ccatcaggct
    82681 caaagcccaa atcttccacc accacaaagt cttccggatt ggctttcagc agcccggtgc
    82741 cttgcggttt accgtggagg taagtgagat tatcaaactc aatcattttg ttgccttaat
    82801 gagtagcgcc accgcttcac aggcaatccc ttccccacgt ccggtaaatc caagtttttc
    82861 cgtagtagtg gctttcacgt taacatcatc catatggcag ccgagatctt cggcaataaa
    82921 cacgcgcatt tgtggaatgt gcggcaacat cttcggtgcc tgagcgatga tagtgacatc
    82981 gacgttgcca aaggtataac ccttcgcctg aatacgacgc caggcttcgc gtagcagctc
    83041 gcggctgtcg gcacctttaa atgccggatc ggtatccggg aacagtttgc cgatatcccc
    83101 cagcgccgcc gcgccaagca atgcatcggt caacgcatga agcgccacgt cgccatcaga
    83161 atgcgccagc aatccttttt cgtaaggaat gcgtacgcca ccaatgataa ttgggccttc
    83221 accgccaaag gcgtgtacgt caaaaccgtg tccaattcgc attatgtatt ctcctgatgg
    83281 atggttcggg tgaggtaaaa ctcggccagt gccaaatctt ccgggcgcgt gactttaatg
    83341 ttatccgcgc ggccttcgac caactgcgga tgaaatccgc aatattccag cgccgaggct
    83401 tcgtcggtaa tggtagcgcc ttcatttaga gcgcgcgtca gacagtcatg taacagctca
    83461 cgagggaaaa attgcggcgt cagcgcgtgc cataagccgt tgcgatcaac ggtatgagca
    83521 atggcatttt tgcccggttc ggcacgtttc atagtatcgc gcactggtgc ggcgaggatc
    83581 cctcccgtgc ggctgttttc gctcaacgcc aacaatcgcg cgaggtcatc ctgatgcaaa
    83641 caaggacgag cggcgtcatg catcaatacc cactgcacat cgccagcggc tttcagacct
    83701 gccagcacgg aatcggcacg ctcatcaccg ccatctacaa cggtgatttg cggatgattc
    83761 gccagaggaa gttgtgcaaa acggctatcg ccaggactta tggcaatgac gacacgtgtc
    83821 acccggggat gcgccagcag cgcatgcacc gagtgttcaa gaatggtttg attaccgatt
    83881 gagagatatt gcttaggaca ttccgtttgc attcgacggc caaatccggc cgccggaacc
    83941 acggcgcaaa catccaaatg agtggttgcc atgttaattc ccgggctgat ttatcgattg
    84001 ttttgccccg cagactgtgc gcgcttcgac gcgtcaggca ccagacgata aaaagtttcg
    84061 cccggcctgg tcatgctgag ttcattacgc gcacgctctt cgagcgcctc ctggccgcca
    84121 ttgagatcgt caatttcggc aaaaagttga tcgtttcgcg ctttaagttt cgcgtttgta
    84181 gcttgcagtg ccgccacatc atcattgacg cgggtatagt catgtatacc gttcttaccg
    84241 aaccacagcg aatactgtag ccagaccaga atagccagca acagcagcgt tagtttaccc
    84301 atcctgcccc ctgaaaaacg gcatcatcat cccatgcatc cgaagacgac tctacatcct
    84361 ctgttgggga taccgcgaca acgcgggcaa atgtaccaca tttgtccatt gttacgtata
    84421 cccagggcgt gcagaacata atctcattat tagttacggt ttgaattatg aacagaggag
    84481 acaagaaagt acaaattagc ccagtagcca cataaacagt gcgccaaaca taatgcctac
    84541 tgtcaccagg gtgaaaacaa tactgtagcg tagctttccg tccatcaatg aatgcagcgc
    84601 aatccccacc actaccgcga caggcatcag tgccagaaaa aaaggccagg tgtagataaa
    84661 gaagaacagc gtgttagagc cataaatcaa catcggcatc gccagcgcaa ataaccagga
    84721 gataaaaccg accacggcac caggcagtga ccatgtagtt tcttcatcct cagtaaggct
    84781 gtcgttattt gttagtgtaa tgttatggct attgcgcata tttgatcctg ttactctgac
    84841 gaaccgggca tggaaacccg gtggtgtctc aggatctgat aatatcgttc tgtctcaaca
    84901 gatctaataa ttgctgtacc aaatttgtta ctaattgttc accattgaga tgaatttctg
    84961 ccgattcagg cgcttcgtaa acggaatcta ttcccgtaaa gttgcgcagt tcaccggcac
    85021 gcgctttctt atataagcct tttggatcgc gggcttcgca aatcgccagc ggcgtatcga
    85081 caaacacttc aataaagcgc ccttctccta cgcgttcgcg aaccatctgg cgttcggcgc
    85141 ggtgtggcga gataaatgcg gtcagcacca ccagtccggc ttcaaccatc aaattcgcca
    85201 cttcaccgac gcgacggata ttctctttac gatcggcatc gctaaaaccg agatcgctgc
    85261 ataatccgtg gcgaacattg tcgccatcca gcagatacgt actgacgccg agtttatgta
    85321 acgcctcctc cagcgccccg gcgaccgttg atttaccgga cccggagagg ccggtaaacc
    85381 acagcactac accacgatga ccgtggtgta gttcgcgttg ttgcacagtg accggatgac
    85441 tatgccagac gacgttttcg tcatgcagcg ccattattta tcccccagca aatcgcgcgc
    85501 gccccagtgc ggaaagtggc gacgaaccag ggcattcaat tccagttcga atgcactgaa
    85561 ttcagatgac gcagctgttg cctggctaac tggctcgtgc accataccgg cacccacggt
    85621 cacattgctc aggcgatcga taaaaatcag cccaccggta accggatttt gctgatagcg
    85681 atctaacacc agtggctcgt caaaagtgag atccaccagg ccgatgccgt tcagcggcag
    85741 gttttcaact tcgcgttggg taaggttatt aatatcaacc tgataacgaa tgccatcaac
    85801 acgagcacgc gtcttcttac cggcaatttt gatgtcataa ctctggccca gggaaagcgg
    85861 ctgttccgcc atccatacca catccaccga cgcactctgc accgccggta acgcttcgtc
    85921 tgccgccagc agcagatcac cacggctgat atcgatctca tccgtcagca ccagggtgat
    85981 cgcttctccg gcaaaggctt cttcgcgatc accatcaaaa gtcacgatcc gcgcgacgtt
    86041 tgattccaca ccagagggca gcacttttac acgttgcccg acttccacgc gaccggatgc
    86101 cagcattccg gcgtagccac gaaaatcgag atttgggcgg ttaacgtact gcaccgggaa
    86161 gcgcattggc tgggcatcca ccactcgctg gatctccacg gtttccagca cttcgagcag
    86221 tgtcggaccg ctgtaccacg gcatactttc actttgcgaa gccacgttgt caccttccag
    86281 tgcggagagc ggcacaaagc ggatatccag attacccggc agctgcccgg caaaggtcag
    86341 ataatcttca cgaatacggg tgaacgtctc ttcactgtaa tccaccagat ccattttgtt
    86401 gatcgccacg accagatgtt tgatccccaa cagtgtggag ataaaactgt gacgacgggt
    86461 ttgatcgagc acgcctttac gggcatcgat cagtaagatc gccagttcac atgtcgatgc
    86521 gccagtcgcc atattgcgag tgtactgctc gtgccctggg gtgtcggcga taataaattt
    86581 acgcttctcg gtagagaaat agcggtaggc cacatcaata gtgatgccct gttcgcgctc
    86641 agcttgcagg ccgtccacca gcagagccag atccagcttt tcgccctggg tgccgtgacg
    86701 cttactgtcg ttatgcagcg atgagagctg atcttcgtag atttggcggg tatcgtgcag
    86761 cagacgacca atcagggtac ttttgccgtc atcgacgcta ccacaggtca gaaaacgcag
    86821 caggctttta tgttgttgcg caatcatcca ggcttcgacg ccgccttcat tggcgatttg
    86881 ttgtgcaagt acggtgttca tcttaaaaat acccctgacg ttttttcagc tccatggatc
    86941 ccgcctggtc gcggtcaatc acgcgaccct gacgttcact ggtggtggaa accagcatct
    87001 cttcgatgat ttccggcagt gtttgtgcat ttgactccac cgcaccggtc agcggccagc
    87061 agcccagcgt acggaaacgc accatccgtt ttttgatcac ttcgcccggt tgcaggtcga
    87121 tacggttgtc atcaatcatc atcaacatac cgtcgcgttc cagaaccgga cgttccgcag
    87181 cgagatacag cggcacaatg tcgatatttt ccagccagat gtattgccag atatcctgct
    87241 cggtccagtt agagagcggg aagacgcgga tgctttcgcc tttgttaatt tgcccgttgt
    87301 agttgtgcca cagttccggg cgctgatttt tcggatccca gcgatggaag cggtcacgga
    87361 aagagtagat tcgctcttta gcgcgggatt tctcttcgtc acggcgcgca ccaccgaagg
    87421 cggcatcaaa accgtatttg ttcagcgcct gtttcaggcc ttcagttttc ataatatcgg
    87481 tatgtttcgc gctgccgtgc acgaatggat taatccccat cgccacgcct tccgggtttt
    87541 tatgcaccag cagctcgcag ccgtaggctt tcgccgtacg atcgcggaac tcatacatct
    87601 cgcggaattt ccagccggta tcgacatgca gcaacgggaa aggcagcgta cctggataaa
    87661 acgccttgcg cgccagatgc agcatgacgc tggaatcttt accgatagag tagagcatca
    87721 ccggatttga gaattctgcc gccacctcgc gaataatgtg gatgctttcc gcctccagtt
    87781 gccgcaggtg agtaagtcgt ttttgatcca taaccgttcc tttgcaatac cgctattttc
    87841 ttgccatcag atgtttctga ctatagggag cgtaagagaa cgaatgaaat taccaattag
    87901 aatgagtagt tccttaacgg aataacgatt tggcaaagct aatatcaaaa agtgcttaag
    87961 gcaccggatt tcgggcgttt aggaagattt gaaattgttt tagcgcagcg gcagtttcat
    88021 actatggcgg taaaaaaatt tgcatggtat ttaaggactc actatgtttt ccgcattgcg
    88081 ccaccgtacc gctgccctgg cgctcggcgt atgctttatt ctccccgtac acgcctcgtc
    88141 acctaaacct ggcgattttg ctaataccca ggcacgacat attgctactt tctttccggg
    88201 acgcatgacc ggaactccag cagaaatgtt atctgccgat tatattcgcc aacagtttca
    88261 gcaaatgggt tatcgcagtg atattcggac atttaatagt cggtatattt ataccgcccg
    88321 cgataatcgc aagaactggc ataacgtgac aggaagtacg gtgattgccg ctcatgaagg
    88381 caaagcgccg cagcagatca tcattatggc gcatctggat acttacgccc cgctgagcga
    88441 tgctgacgca gatgccaatc tcggcggact gacgttacaa ggaatagatg ataacgccgc
    88501 aggtttaggt gtcatgctgg aactggcaga acgcctgaaa aatacgccta ccgagtatgg
    88561 tattcgattt gtggcgacca gcggcgaaga ggaaggaaaa ttaggcgctg agaatttact
    88621 caagcggatg agtgacaccg aaaagaaaaa tacgctgctg gtgattaatc tcgataactt
    88681 aattgttggc gataaattgt atttcaacag cggtgtaaaa ccccctgaag cagtaaggaa
    88741 attaacgcgc gacagggcgc tggcaattgc acgcagtcac ggaatagccg caacgaccaa
    88801 tccgggtttg aataaaaatt atccgaaagg cactgggtgt tgtaatgacg cagaaatatt
    88861 cgacaaagcg ggcattgctg tactttcggt ggaagcgact aactggaatc ttggaaataa
    88921 ggatggttat cagcaacgcg caaaaacacc tgccttcccg gcgggaaata gctggcatga
    88981 cgtaagactg gataatcaac aacatattga taaggcactt cctggaagaa tagaacgtcg
    89041 ctgccgtgac gttatgcgga taatgctacc gttggtgaag gagctggcga aggcgtcttg
    89101 atgggtttga aaatgggagc tgggagttct accgcagggg cggggaattc taagtgatac
    89161 ccatcatcgc atccagtgcg tccggtttac ttccgggaaa accgcaccgt attgcgccgg
    89221 tttatctccg ctggagcggg gaacacctct ggcgaataga aagcaagcca ggttttgacc
    89281 agtcgatcgt acatggcatt ggccgttgcc attgttggca ggccaaaaaa cagcccctga
    89341 gcgttacccg cagccatcaa cctgtgcgcc aggataagcg ccgcttctgt tttacctgcg
    89401 ccggtcacat cttccagaat aaacaactgt ggtcctggct gattgatatc cagatccagc
    89461 accttttgct gtaacggtgt cgtattctca ataaaaggaa acagcgtatt aattccggtg
    89521 aaaggcgcgg tttctgcttg tggcggaaag acggttaatg cgttttgagc ctgaactaaa
    89581 gccttttgcc agtaatcttc aatatccatt gcctgtgcga cgcgtggaaa aaatcgcgtt
    89641 gacgaccctg tccagtctgc aagtacgacg gttgcagaga tataccagga aagttgtttt
    89701 ataagttcaa cgccctcgtc atcatcccag aatgtgggga tctctattcg cggaaacagt
    89761 ggcttgattt caaggagaaa atctcgtgct gcagctttgt cttcaggcag aaaattatcc
    89821 aattcatcca tacgatcagg gggccgacca tgatgccctg tcgtcacggg catccacatt
    89881 tctatcactc gcctaagttt gcgcggagag agcgaagatg aaggaagtaa ctcctgacat
    89941 tcacttaaat aatgattcca caaccagtag cccagcgttg aatgagagat tttttcgtaa
    90001 ttttttctgg caccttcctg aatcttgaat tcagggggca ggtaaagttg ttgaaaagag
    90061 cgggcaaatt ttccaatatc gtgccagcac agcaaccagg cgaaaaattg agctgcctgt
    90121 tccttgtcag aaatccctaa ttgacgaaaa taatcagcca gcccgaagca atttctttta
    90181 accattaaat agcccattgc ggccacatcc agcaaatgcc agcaaagaag gtgatagtcg
    90241 tcgctaccct ctttctcgcc acgtcgggtt tttccccaga aatcaaagaa agtcacaata
    90301 tttttatcct tcagtaaact taaaggatat ttacgcatat tttaaaaatt atctgtgata
    90361 tatatcagcc aataaaacat ttacattatg aataaattaa tgattttcat ttgaaattca
    90421 taataataaa tagagactat atattcacat aaaaaatata aattttaaaa aagggtgcag
    90481 gagaccaaac catcacaatt cagtacgcgg ggttttaccc ccgcgtggtg gctacttctt
    90541 cgattcgtaa gcgagaactg ccttaaactc cataccacgt tctctgatac tcaactcaca
    90601 taacgagtca cctaccatca acgtgaatcc cagtactgta caacacagta cgatgattgc
    90661 cacaagggca tattttgtca gcatgtcttg cctccctgca aagaagagac taacatccga
    90721 attgctacgt tcgaacgcga gcctcaggtt gatagaaata tcgcctgggg cttttgtcca
    90781 tctggaacct cgcgaatgct taacgccaga cagcctcaag cacccaacgc cattctatac
    90841 ctgttaattt tccctgcgat gttcaccttt aaatgccgga atgctgctcg caaaataaaa
    90901 acgcccccat catttctgac agaggcgttt aatttatccg gcaatattta cccttcgtgt
    90961 aatccacatt ccctttttaa gccaaagaaa cgcgtttctt cttccgccat gccgggttcc
    91021 catttacggg ttgtatgggt atcgcccacc gataaatatc cttcgtccca taatgggtga
    91081 tatttcaggc catgtttttg caggtactga taaatagttc ggttatccca gtcgataatc
    91141 ggcagcactt taaatacgcc acgctgaatt gccagcaccg gtaaattggc acggctgccg
    91201 gattgttcac gccgcaggcc agcaaaccac gtttgcgcat tcagttcttt cagcgcccgg
    91261 ttcatcggtt cgactttgtt gatgtcattg tacttttcaa tgccttcaac gccctgttcc
    91321 cacagtttgc cgtagcgtgc ttcctgccag gcggcgcttt cggtagcgcg gtacactttc
    91381 aggttgagct tgagtttgtc cgttaactcg tcaataaagc ggtaggtttc cgggaacaga
    91441 tagccggtgt cggtgaggat caccggaata tccgggtgaa tctgattcac cagatgcagg
    91501 ctcaccgccg cctgaatgcc aaagctggaa gaaagtacat attcaccggg cagattatcc
    91561 agcgcccagg caacgcggcc ttcagcgtcc agtttttcca gttcggcgtt agtttctgcc
    91621 agcgccagaa tgcgatctac cttcggcagt tcgttcaggg cgtttagatc gagtttggac
    91681 atagattcct cactgtttgc cttgcctgat gcgacgctca cgcgtcttat caggcctaca
    91741 agaccgggct gatggttaat cccacaaatc gcgcgccgga tcgagcaccg ggcgaatgat
    91801 gcccgcacgc accgtaaagt cgccgaagcc ttcacccgct tcgcgctctt tcgcccagcg
    91861 ccctatcagt tcatcaagcg acgtcaggat ttccggctcg gtgatgtttt ctttatacat
    91921 ccgtgggata cgtgtcccaa tgcggttgcc gccaagatgc aggttgtagc gacccggtgc
    91981 tttacccacc aggcccactt ccgccagcat cgcgcgacca caaccgttcg ggcagcctgt
    92041 tacacgcatc acgatatgct catcgctgac accatgtttc gccattaaat tatcgatgtt
    92101 gtcgataaaa gacggcagga aacgctccgc ttccgccatc gccagcgggc aagtcgggaa
    92161 tgacacgcag gccatcgagt tttcacgctg cggcgtgacg gcattcatta acccgctctc
    92221 tttggcaatt ttctcgatct tcgctttctc gctttccggc acaccggcga tgatcagatt
    92281 ctggttcgcc gtaatgcgga aatcaccttt gtggatcttc gcgatctcca gcaggccagt
    92341 tttcagcgga cgccccggat aatcaaggat gcgaccattt tcgataaaca gcgtaaggtg
    92401 ccagttatca tcaatgccct taacccagcc aatacgatcg ccgcgccctg taaattcata
    92461 cgggcggatc ggttcaaatt tgatccccgc gcgacgctcc acttccgctt taaacgtctc
    92521 aacccccacg cgctccagtg tgtatttggt tttggcattt ttacgatcgg ttcggttacc
    92581 ccagtcacgc tgagttgtca cgacggcttc cgccaccgct agcgtatgct ctagcggcag
    92641 atagccaaac tcactcgccg tgcgggcgta ggttttcttg ttaccgtgtt cgatagaaag
    92701 cccaccgccc accagcaggt tgaagcccac cagcttgccg ttttccgcga tcgccacgaa
    92761 gttcatgtcg ttggcgtgca gatcaatatc gttctgcggc gggatcacta ccgtggtttt
    92821 gaacttacgc ggcaggtagg tctgaccaag gatcggttct tcatcggtgg tggcgacttt
    92881 ttcctgatcg agccagatct ccgcatacgc gcgggtacgc ggcagcagat gttcagaaat
    92941 cttcttcgcc cactcgtacg cttctgcgtg cagctgcgac tcgtaagggt tcgaggtgca
    93001 gagtacgtta cggttcatgt cgttagcagt cgccagcgca tcaagaccga ccgagtgcag
    93061 catctggtgc accggtttga cgttcttttt cagaatgccg tggaactgaa acgtctggcg
    93121 gttggtcagg cgaatgctgc catagatggt gttttcaccg gcaaatttat cgatcgcctg
    93181 ccactgtttg gtggtgataa ctccacccgg cagacggcag cgcaacagca tcgcgtggcg
    93241 cggctccagc ttctgttcag cacgttcggc gcggatgtcg cggtcatcct gctgatacat
    93301 gccgtggaag cggatcagca ggaagttatc gcccttaaag ccgccggtca gaccgtcgtt
    93361 taaatcttcc gcaatggtgc cgcgcaggta gttgctttca agcttcatgc gctcggcgtc
    93421 tgtcagtttt ccttcgacca ctaaaggccc tggatgtttt tcgctcatta gtagacatct
    93481 cgctgataac gacgctctac gcgcagctca cttaaaaatt catccgccgc ttcggtgtcc
    93541 atgccaccaa attcggcaat cacttccaga agtgcctgct caacgtcttt cgccatgcga
    93601 ttagcgtcgc cgcagacata aatgtgggca ccgtcattga tccagcgcca cagctccgcg
    93661 ccctgttcgc gcagtttatc ttgtacgtaa actttttctt tttggtcgcg cgaccaggcg
    93721 agatcgatcc gcgtcagcac accctctttg acgtagcgct gccactccac ctgatacagg
    93781 aagtcttccg taaaatgcgg attgccaaag aacagccagt ttttacctgg cgcttcgtca
    93841 gcggcgcgtt gctgcataaa ggcgcggaac ggcgcaatac cggtgcctgg gccaatcata
    93901 atcaccgggg tttccaggtt agcgggcagg cggaagttat cgttatgttc gataaatacg
    93961 cggacttcgc cctcttcttc cacgcggtca gcgaggaagc tggaggcacc accggcacgg
    94021 gcgcggcctt ccacgtcgta acgcaccaca ccaacggtga cgtgtacttc gttctcgact
    94081 tccgcctgcg aggaggcaat ggaatagaga cgcggggtca gcgggcgcag cagattaatt
    94141 agcgcttcgg catcaagctg tgccggggag aaacgcacca tgtcaacaat cggcgtcgtc
    94201 gcggcgtaat gctgtaactt cgctttatcg cccaccagcg gcagcagtgt ttcactgcgg
    94261 gtaagcgtgg cgtaattctc aacaatgttg gcggtgttga cggtcagttc gaagtgccac
    94321 tgtagcgctt cgttcagagg caacgttttg ccctcgacag tgacaggttc atcgcctgtc
    94381 agccacagca gttcgacaag ttctttcacc agtgccggat cgttctgata ccagacgccc
    94441 agcgcgtcac ccggctggta acgcaggccc gagtcgccta agtcaatttc gatatggcga
    94501 acgtcttttt cagagttacg cccagtaatt ttctggttaa cagagaggct agccaccagc
    94561 ggcgcgtctt tgctgtacgg gctggtgtgg atttcattta ccgcgccagt agcgacggat
    94621 tgcgaaggtg ccgcgacagg cgcacgcgat ttaagcgcat caaccacgcg ggcgcgccac
    94681 tcgctggcag cagcctggta ttcaacatca gcatcgacac ggtcgagcag gcgttcacca
    94741 cccagttccg ccagcttgct gtcaaaatct ttcccggact ggcagaaaaa ttcataagag
    94801 ctatcgccga ggctaaacac ggcaaacgcg gtgttttcca gctttggcgc ttttttggag
    94861 aacaggaact tatgcagcgc gacggcttct tccggcggtt ccccttcccc ttgcgttgac
    94921 gtcactacga tgagcagttt ttcgctggcg atttgtttga atttatagtc gcccgcgttc
    94981 accagcttaa cgttcagttt tgctgctaat aaatcatcgc gtaatgcttc agcaacccgg
    95041 cgcgcattgc cggtttgcga agcagagata atcgttatac ccggcatttc tgcggcgggc
    95101 gctggcgtca ctgcaagcgc aacaggctgc tggttgagca cgccccagaa atagccagaa
    95161 acccaggcaa gctgggtagg cgttaaatcg gtcgtggccg cctgaaggcg tgccagttgc
    95221 tccgggttca acggaagcaa cgcggaaggt gggacctgtg tcgtcatgcg tcgttatgtt
    95281 ccagtaagca aagctgtttc tgcgccctgt cagcgcccat aaaacagaag agaaggtaag
    95341 gttaacgggg tgaacggtgt ggattaaaga cgggatagcg ataactaata accaaatcga
    95401 ctaacctgtt ttagcaatag tctttaacaa caaaatagat taaccaacct aatgaaaaac
    95461 aaatgaattt agccaatcat taagataaat cagcgatttt gcgcaacaaa gtcgttttag
    95521 ataatgcgaa aaaacagcct ttccggtact ctacggcggt tttatcgtcc tgtagagaaa
    95581 ttatgatgtc caccacgtta tttaaagatt tcaccttcga agccgctcac cgcttaccac
    95641 atgtcccgga agggcataaa tgtggtcgcc tgcacgggca ttcctttatg gtgcgactgg
    95701 aaattaccgg ggaagtcgat ccgcatacgg gctggattat cgatttcgct gaactgaaag
    95761 cggcgtttaa accaacttat gagcgcctcg atcaccatta tctcaatgat attccaggtc
    95821 tggaaaaccc aaccagcgaa gttttagcaa aatggatttg ggatcaggtt aaacccgttg
    95881 tgccgctgtt aagtgcggtg atggtaaaag aaacctgcac cgcagggtgt atctatcgcg
    95941 gcgaatgata agagtgtgtc ggcggtcaat ttccctttag taacgctatg ttagggtgtt
    96001 gtgttctgga tatctggggc atgacatgga agacgactgc gacattatta ttattggtgc
    96061 cggtattgca ggcaccgctt gcgcgttacg ctgcgcgcga gcggatttat ccgttttgtt
    96121 actcgaacgc gctgaaatcc ccggcagcaa aaatctttcc ggcgggcggt tatataccca
    96181 tgcactcgcg gaactcctcc cgcaatttca tctgaccgcg cctcttgaac gacgcatcac
    96241 tcacgaaagc ctttccctgt taacgccgga tggcgcaacg acgttttcca gcttacagcc
    96301 cggcggtgaa tcctggagtg tattacgtgc acggttcgat ccgtggctgg ttgccgaagc
    96361 cgaaaaagaa ggtgtcgaat gcatccccgg agcgacggtg gatgcactgt atgaagaaaa
    96421 cggcagagtc tgtggcgtta tttgtggtga cgatattctc cgcgcccgtt atgtggtgct
    96481 ggcagaaggt gccaacagcg tcctggctga acgtcacggg ttagtgactc gtcctgctgg
    96541 cgaagcgatg gcgttgggga tcaaagaagt gctgtcgctg gaaacatccg ctattgaaga
    96601 acgttttcat ctggagaata acgaaggcgc agcgttgctg ttcagcggcg gaatctgtga
    96661 tgacttaccc ggcggcgcat ttctttatac taatcaacaa acgctctcgt tagggattgt
    96721 ttgcccgctc tcttccctta cgcaaagtcg tgttccggca agcgagctgc tgactcgctt
    96781 taaagcgcat ccggcagtgc gcccgcttat caaaaacacg gaatcactgg agtatggtgc
    96841 gcatctggtg ccagaaggtg gcttgcacag tatgccggtg caatacgccg gtaacggctg
    96901 gctgctggtg ggcgatgcgt tgcgcagttg cgtcaatacc ggaatttccg tgcgcggcat
    96961 ggatatggcg ctgactggcg cacaggcggc ggcacaaacg ctaataagcg cctgccagca
    97021 ccgcgagccg caaaatctgt ttgcgcttta tcatcacaac gtagagcgca gcctgctatg
    97081 ggatgttcta caacgttatc agcatgttcc ggcgcttttg caacccctgg ctggtatcgg
    97141 gcgtggcctg cgttaatgca ggatatttcc cgcgatttat gggatcaggg tgataaacct
    97201 gttccaccgc tgcgccagtt attctggcgt catttacgtc gtcatggcct gtggcatctg
    97261 gcgggcgatg ttatcaggag tctgcgatgt ctgtagcccg taatctctgg cgcgttgctg
    97321 atgcgccgca cattgttccg gctgactccg tt
//
LOCUS       DBKEKI010000002        77854 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.002, whole genome shotgun sequence.
ACCESSION   DBKEKI010000002 DBKEKI010000000
VERSION     DBKEKI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 77854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.002"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..2265
                     /locus_tag="ACUQPX_000108"
                     /pseudo
     CDS             1..2265
                     /locus_tag="ACUQPX_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2266..3915
                     /locus_tag="ACUQPX_000109"
     CDS             2266..3915
                     /locus_tag="ACUQPX_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HHV9054919.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELVYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3920..4696
                     /locus_tag="ACUQPX_000110"
     CDS             3920..4696
                     /locus_tag="ACUQPX_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HHV9054920.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4971..7820
                     /gene="rcsC"
                     /locus_tag="ACUQPX_000111"
     CDS             4971..7820
                     /gene="rcsC"
                     /locus_tag="ACUQPX_000111"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HHV9054921.1"
                     /translation="MKYLASFRTTLKASHYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8020..8670)
                     /gene="rcsB"
                     /locus_tag="ACUQPX_000112"
     CDS             complement(8020..8670)
                     /gene="rcsB"
                     /locus_tag="ACUQPX_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HHV9054922.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8687..11359)
                     /gene="rcsD"
                     /locus_tag="ACUQPX_000113"
     CDS             complement(8687..11359)
                     /gene="rcsD"
                     /locus_tag="ACUQPX_000113"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HHV9054923.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTLRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESDVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            12098..13219
                     /gene="ompC"
                     /locus_tag="ACUQPX_000114"
     CDS             12098..13219
                     /gene="ompC"
                     /locus_tag="ACUQPX_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HHV9054924.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13331..14386
                     /gene="apbE"
                     /locus_tag="ACUQPX_000115"
     CDS             13331..14386
                     /gene="apbE"
                     /locus_tag="ACUQPX_000115"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HHV9054925.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14460..15524
                     /gene="ada"
                     /locus_tag="ACUQPX_000116"
     CDS             14460..15524
                     /gene="ada"
                     /locus_tag="ACUQPX_000116"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HHV9054926.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15524..16174
                     /gene="alkB"
                     /locus_tag="ACUQPX_000117"
     CDS             15524..16174
                     /gene="alkB"
                     /locus_tag="ACUQPX_000117"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HHV9054927.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            16250..17893
                     /gene="yojI"
                     /locus_tag="ACUQPX_000118"
     CDS             16250..17893
                     /gene="yojI"
                     /locus_tag="ACUQPX_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HHV9054928.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            18111..19757
                     /gene="mqo"
                     /locus_tag="ACUQPX_000119"
     CDS             18111..19757
                     /gene="mqo"
                     /locus_tag="ACUQPX_000119"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HHV9054929.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGGAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNRHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(19907..20416)
                     /gene="eco"
                     /locus_tag="ACUQPX_000120"
     CDS             complement(19907..20416)
                     /gene="eco"
                     /locus_tag="ACUQPX_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HHV9054930.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRIWKAE
                     EKIDNAEVR"
     gene            complement(20530..20695)
                     /gene="yojO"
                     /locus_tag="ACUQPX_000121"
                     /pseudo
     CDS             complement(20530..20695)
                     /gene="yojO"
                     /locus_tag="ACUQPX_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
     gene            20804..21298
                     /gene="napF"
                     /locus_tag="ACUQPX_000122"
     CDS             20804..21298
                     /gene="napF"
                     /locus_tag="ACUQPX_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HHV9054931.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            21288..21551
                     /gene="napD"
                     /locus_tag="ACUQPX_000123"
     CDS             21288..21551
                     /gene="napD"
                     /locus_tag="ACUQPX_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HHV9054932.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQQNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            21548..24034
                     /gene="napA"
                     /locus_tag="ACUQPX_000124"
     CDS             21548..24034
                     /gene="napA"
                     /locus_tag="ACUQPX_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HHV9054933.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            24041..24736
                     /gene="napG"
                     /locus_tag="ACUQPX_000125"
     CDS             24041..24736
                     /gene="napG"
                     /locus_tag="ACUQPX_000125"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HHV9054934.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            24723..25586
                     /gene="napH"
                     /locus_tag="ACUQPX_000126"
     CDS             24723..25586
                     /gene="napH"
                     /locus_tag="ACUQPX_000126"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HHV9054935.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            25583..26032
                     /gene="napB"
                     /locus_tag="ACUQPX_000127"
     CDS             25583..26032
                     /gene="napB"
                     /locus_tag="ACUQPX_000127"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HHV9054936.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            26042..26644
                     /gene="napC"
                     /locus_tag="ACUQPX_000128"
     CDS             26042..26644
                     /gene="napC"
                     /locus_tag="ACUQPX_000128"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HHV9054937.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVELGF"
     gene            26657..27280
                     /gene="ccmA"
                     /locus_tag="ACUQPX_000129"
     CDS             26657..27280
                     /gene="ccmA"
                     /locus_tag="ACUQPX_000129"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HHV9054938.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            27277..27939
                     /gene="ccmB"
                     /locus_tag="ACUQPX_000130"
     CDS             27277..27939
                     /gene="ccmB"
                     /locus_tag="ACUQPX_000130"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HHV9054939.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            27981..28718
                     /gene="ccmC"
                     /locus_tag="ACUQPX_000131"
     CDS             27981..28718
                     /gene="ccmC"
                     /locus_tag="ACUQPX_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HHV9054940.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            28715..28924
                     /gene="ccmD"
                     /locus_tag="ACUQPX_000132"
     CDS             28715..28924
                     /gene="ccmD"
                     /locus_tag="ACUQPX_000132"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HHV9054941.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            28921..29400
                     /gene="ccmE"
                     /locus_tag="ACUQPX_000133"
     CDS             28921..29400
                     /gene="ccmE"
                     /locus_tag="ACUQPX_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HHV9054942.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            29397..31340
                     /gene="ccmF"
                     /locus_tag="ACUQPX_000134"
     CDS             29397..31340
                     /gene="ccmF"
                     /locus_tag="ACUQPX_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HHV9054943.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            31337..31894
                     /gene="dsbE"
                     /locus_tag="ACUQPX_000135"
     CDS             31337..31894
                     /gene="dsbE"
                     /locus_tag="ACUQPX_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HHV9054944.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            31891..32943
                     /gene="ccmH"
                     /locus_tag="ACUQPX_000136"
     CDS             31891..32943
                     /gene="ccmH"
                     /locus_tag="ACUQPX_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HHV9054945.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(33154..33801)
                     /gene="narP"
                     /locus_tag="ACUQPX_000137"
     CDS             complement(33154..33801)
                     /gene="narP"
                     /locus_tag="ACUQPX_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HHV9054946.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            34121..36712
                     /gene="yejO"
                     /locus_tag="ACUQPX_000138"
     CDS             34121..36712
                     /gene="yejO"
                     /locus_tag="ACUQPX_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HHV9054947.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKMQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            complement(36815..36891)
                     /locus_tag="ACUQPX_000139"
     tRNA            complement(36815..36891)
                     /locus_tag="ACUQPX_000139"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36855..36857),aa:Pro,seq:ggg)
     gene            complement(36966..38726)
                     /gene="yejM"
                     /locus_tag="ACUQPX_000140"
     CDS             complement(36966..38726)
                     /gene="yejM"
                     /locus_tag="ACUQPX_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HHV9054948.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(38746..38973)
                     /locus_tag="ACUQPX_000141"
     CDS             complement(38746..38973)
                     /locus_tag="ACUQPX_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HHV9054949.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQASINEDKAH"
     gene            39155..40162
                     /gene="yejK"
                     /locus_tag="ACUQPX_000142"
     CDS             39155..40162
                     /gene="yejK"
                     /locus_tag="ACUQPX_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HHV9054950.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(40301..40585)
                     /gene="rplY"
                     /locus_tag="ACUQPX_000143"
     CDS             complement(40301..40585)
                     /gene="rplY"
                     /locus_tag="ACUQPX_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HHV9054951.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(40710..42470)
                     /locus_tag="ACUQPX_000144"
     CDS             complement(40710..42470)
                     /locus_tag="ACUQPX_000144"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HHV9054952.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            42620..43315
                     /gene="rsuA"
                     /locus_tag="ACUQPX_000145"
     CDS             42620..43315
                     /gene="rsuA"
                     /locus_tag="ACUQPX_000145"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HHV9054953.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            43343..44533
                     /gene="bcr"
                     /locus_tag="ACUQPX_000146"
     CDS             43343..44533
                     /gene="bcr"
                     /locus_tag="ACUQPX_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HHV9054954.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSV
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            44866..45210
                     /locus_tag="ACUQPX_000147"
     CDS             44866..45210
                     /locus_tag="ACUQPX_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HHV9054955.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(45214..46803)
                     /gene="yejF"
                     /locus_tag="ACUQPX_000148"
     CDS             complement(45214..46803)
                     /gene="yejF"
                     /locus_tag="ACUQPX_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HHV9054956.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLLVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRITIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(46805..47830)
                     /locus_tag="ACUQPX_000149"
     CDS             complement(46805..47830)
                     /locus_tag="ACUQPX_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9054957.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITILFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(47830..48924)
                     /locus_tag="ACUQPX_000150"
     CDS             complement(47830..48924)
                     /locus_tag="ACUQPX_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HHV9054958.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(48925..50739)
                     /locus_tag="ACUQPX_000151"
     CDS             complement(48925..50739)
                     /locus_tag="ACUQPX_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HHV9054959.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDCLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(50821..52377)
                     /locus_tag="ACUQPX_000152"
     CDS             complement(50821..52377)
                     /locus_tag="ACUQPX_000152"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HHV9054960.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAEFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(52558..53124)
                     /gene="mepS"
                     /locus_tag="ACUQPX_000153"
     CDS             complement(52558..53124)
                     /gene="mepS"
                     /locus_tag="ACUQPX_000153"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HHV9054961.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(53536..54249)
                     /gene="lpxT"
                     /locus_tag="ACUQPX_000154"
     CDS             complement(53536..54249)
                     /gene="lpxT"
                     /locus_tag="ACUQPX_000154"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HHV9054962.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALTIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(54288..55274)
                     /gene="yeiR"
                     /locus_tag="ACUQPX_000155"
     CDS             complement(54288..55274)
                     /gene="yeiR"
                     /locus_tag="ACUQPX_000155"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HHV9054963.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(55392..56858)
                     /locus_tag="ACUQPX_000156"
     CDS             complement(55392..56858)
                     /locus_tag="ACUQPX_000156"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HHV9054964.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYISGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(57081..57653)
                     /gene="yeiP"
                     /locus_tag="ACUQPX_000157"
     CDS             complement(57081..57653)
                     /gene="yeiP"
                     /locus_tag="ACUQPX_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HHV9054965.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            57808..58062
                     /locus_tag="ACUQPX_000158"
     CDS             57808..58062
                     /locus_tag="ACUQPX_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HHV9054966.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(58059..59246)
                     /gene="setB"
                     /locus_tag="ACUQPX_000159"
     CDS             complement(58059..59246)
                     /gene="setB"
                     /locus_tag="ACUQPX_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HHV9054967.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALALAREHADKTGREAVMFSSFLRAQVSLAWVI
                     GPPLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRR
                     DTLLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGY
                     FAKRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQD
                     LMPGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRI
                     KDV"
     gene            59614..60744
                     /gene="fruB"
                     /locus_tag="ACUQPX_000160"
     CDS             59614..60744
                     /gene="fruB"
                     /locus_tag="ACUQPX_000160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HHV9054968.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            60744..61682
                     /gene="fruK"
                     /locus_tag="ACUQPX_000161"
     CDS             60744..61682
                     /gene="fruK"
                     /locus_tag="ACUQPX_000161"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HHV9054969.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            61699..63390
                     /gene="fruA"
                     /locus_tag="ACUQPX_000162"
     CDS             61699..63390
                     /gene="fruA"
                     /locus_tag="ACUQPX_000162"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HHV9054970.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            63814..64755
                     /locus_tag="ACUQPX_000163"
     CDS             63814..64755
                     /locus_tag="ACUQPX_000163"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HHV9054971.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            64743..65681
                     /gene="psuG"
                     /locus_tag="ACUQPX_000164"
     CDS             64743..65681
                     /gene="psuG"
                     /locus_tag="ACUQPX_000164"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HHV9054972.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            65775..67025
                     /locus_tag="ACUQPX_000165"
     CDS             65775..67025
                     /locus_tag="ACUQPX_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HHV9054973.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLLFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAILPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(67096..67794)
                     /locus_tag="ACUQPX_000166"
     CDS             complement(67096..67794)
                     /locus_tag="ACUQPX_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HHV9054974.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKHLSGLA
                     LEMDPENKFSGMMQ"
     gene            67925..68866
                     /gene="rihB"
                     /locus_tag="ACUQPX_000167"
     CDS             67925..68866
                     /gene="rihB"
                     /locus_tag="ACUQPX_000167"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HHV9054975.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVVGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            68966..70216
                     /locus_tag="ACUQPX_000168"
     CDS             68966..70216
                     /locus_tag="ACUQPX_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HHV9054976.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(70272..71361)
                     /locus_tag="ACUQPX_000169"
                     /pseudo
     CDS             complement(70272..71361)
                     /locus_tag="ACUQPX_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(71364..72221)
                     /gene="nfo"
                     /locus_tag="ACUQPX_000170"
     CDS             complement(71364..72221)
                     /gene="nfo"
                     /locus_tag="ACUQPX_000170"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HHV9054977.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(72295..73344)
                     /locus_tag="ACUQPX_000171"
     CDS             complement(72295..73344)
                     /locus_tag="ACUQPX_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HHV9054978.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            73443..74324
                     /gene="yieE"
                     /locus_tag="ACUQPX_000172"
     CDS             73443..74324
                     /gene="yieE"
                     /locus_tag="ACUQPX_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HHV9054979.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            74530..75999
                     /gene="lysP"
                     /locus_tag="ACUQPX_000173"
     CDS             74530..75999
                     /gene="lysP"
                     /locus_tag="ACUQPX_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HHV9054980.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            76291..>77854
                     /gene="cirA"
                     /locus_tag="ACUQPX_000174"
     CDS             76291..>77854
                     /gene="cirA"
                     /locus_tag="ACUQPX_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HHV9054981.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGIKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYY"
BASE COUNT        18731 a        19051 c        20994 g        19078 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct acgaccaggc
      181 gctctccagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgataa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgatgc cttccttacg gcatgggcat ggtactccga
     1081 ctggcaggcc agccaggcgc tcggcgtaac gcaacaatcg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cggataacat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaagggttgg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtca gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaaatg tgcaggtccg ctggcgtgaa ccggcaaaaa cggctcaaca aagtaacatt
     1801 ccggtgaccg ttgaacgcca gttgtatcgg cttatccccg gtgaagaaga gatgagcttt
     1861 actctgcagc cggtgaccag caatgagatt gacagcgatg cgctgtatct cgatgaaatt
     1921 acgcttacca gcgagcagga tgcagttctg cgctatggtc aggtggaagt accgctcccg
     1981 ccgggagccg acgttgagcg cacaacatgg ggcatttcag tcaataaacc caacgccgcg
     2041 aaacagcagg ggcaattgct ggaaaaagcg cgtaatgaaa tgggcgaact ggcctatatg
     2101 gtgccggtga aagaactgac gggaacggtc actttccgcc atttgctgcg cttctcgcaa
     2161 aaagggcaat tcgttctgcc tcctgctcgt tatgtgcgtt cctatgcacc tgcgcagcaa
     2221 agtgtcgcgg cagggagtga atggaccggg atgcaggtga aataagtgaa ctggcgcaga
     2281 atcgtctggc tattggcgtt ggttactctg ccaacgcttg cggaggagac gccgttacaa
     2341 ctggtgctgc gcggtgcgca gcacgatcaa ctgtatcagc tgtcgtcatc aggcgtgacc
     2401 aaagtctcag cattacctga ctcgctcacc acgccgttag ggagtttgtg gaaactgtat
     2461 gtttacgcct ggctggagga tacccatcaa ccggagcagc cctaccagtg tcgcggaaac
     2521 tccccggaag aggtttattg ttgtcaggct ggggagagca taacgcgcga taccgcgctg
     2581 gtacgttcct gtgggctcta ttttgcgccg cagcggttgc atattggcgc agatgtgtgg
     2641 gggcaatact ggcaacagcg tcaggcacct gcgtggttag cgtctcttac aacgctgaag
     2701 ccagaaacct cggtaacggt taaatcgctg cttgattcat tagcgacgtt acctgcgcaa
     2761 aacaaagctc aggaagtctt actggatgtg gtgctggacg aggcgaaaat cggtgtcgca
     2821 tccatgctcg gtagtcgggt acgggtgaaa acctggagct ggtttgccga tgataaacag
     2881 gaaatccgtc agggtggttt tgctggctgg ttaaccgatg gtacaccgct gtgggttact
     2941 ggtagtggca ccagcaaaac ggtactaacc cgttatgcga cggtgcttaa tcgggttttg
     3001 ccagtgccga cgcaggttgc cagtgggcaa tgtgtagagg tggagctgtt tgcccgctat
     3061 ccgctgaaga aaatcacagc ggaaaagagt acgacggcag taaaacccag cgtacttaat
     3121 gggcgctatc gtgtgacgtt tactaatggt aaccacatta cgtttgtcag ccacggtgag
     3181 acgaccttac tgagcgaaaa gggcaaactg aaattgcagt cgcatctcga tcgtgaagag
     3241 tacgttgcca gagttctgga tcgcgaggcg aaaagtacgc cacctgaggc agcgaaagcc
     3301 atgaccgtgg cgatacgtac ttttttacag caaaacgcca atcgggaagg tgattgcctg
     3361 accatccccg acagcagcgc cacgcagcgt gtttctgctt cgccagcgac cacgggggcg
     3421 cgaacgatga ccgcctggac gcaggagctc gtctatgcag gcgatccggt tcattatcat
     3481 ggcagtcgcg ctaccgaagg gacactttcc tggcggcaag ccacggccca ggcggggcag
     3541 ggggagcgtt acgaccagat actcgcgttt gcttatcccg acaacagcct tagccgctgg
     3601 ggagctccac gctcaacctg ccagttatta cccaaagcga aagcctggct ggcgaaaaaa
     3661 atgccgcagt ggcggcgtat attacaaggc gagacggggt acaacgaacc agacgtgttt
     3721 gcggtctgtc gtttagtctc aggtttcccc tataccgatc ggcagcagaa acggctgttt
     3781 attcgtaact tctttacgct tcaggatcgg ctggatttaa cccatgagta tctgcacctg
     3841 gcctttgatg gttatcccac cgggcttgat gagaactata tcgaaacgct gacccgacaa
     3901 ttattaatgg actaatgcta tgcgaaagat ttttcttccg ttgttactgg tggcactttc
     3961 gcctgtcgct cacagtgaag gtgtgcagga agtcgagatt gacgcaccgc tctctggctg
     4021 gcatccggca gagggcgaag atgccagttt ttcacaatcg atcaattacc cggcgtcgtc
     4081 cgtcaacatg gcagatgatc aaaatatctc agcgcagatc cgcggcaaaa taaagaatta
     4141 tgctgcggcg ggtaaagttc agcagggccg gctggtggtc aacggtgcca gtatgccgca
     4201 gagaattgaa tccgatggtt catttgcacg cccttatatt ttcactgaag gcagcaacag
     4261 cgtgcaggtc atcagcccgg atgggcaaag ccggcaaaaa atgcagtttt actcaacgcc
     4321 gggtacagga actattcgtg cacgtttacg gctggttctc tcgtgggata cggacaatac
     4381 cgatctcgat cttcatgttg tcacgcccga tggcgaacac gcctggtacg gtaacaccgt
     4441 gctgaaaaac agtggtgcac tggatatgga tgtcacgacg gggtacggac ccgagatttt
     4501 cgccatgcca gcaccagttc acgggcgcta tcaggtgtat atcaactact atggtgggcg
     4561 cagcgaaacg gaattgacga ccgcccaact gacgctgatc accgatgaag gatcggtcaa
     4621 tgagaaacag gaaacattta ttgtaccgat gcgtaatgct ggcgagctga cgctggtgaa
     4681 aagttttgac tggtgatcca tcccttcaca caacctggct ctctcttttt tcagataaga
     4741 ctgctgggcg atctgctgac gccaggatat aaccattaac aagcgtcggc agattggtca
     4801 tttcttattt ttcgtactga gaactgtcca tctactccgg ctttcttggt taaggcaatg
     4861 gtttctaagc gatacgcatt attgcaatgg tgacagaaca ttgcggtaac acgcttttac
     4921 cgctacctta accacactcc atcggtcacc tgaggcggag cttcgcccct ttgaaatacc
     4981 ttgcttcttt tcgtacaacc ctgaaagcct cgcactacat gttcagagca ttggcgttag
     5041 tgctctggct gttgattgct ttttcatccg ttttttacat cgttaatgcg ttacatcagc
     5101 gagaatcgga aattcgtcag gaatttaatc tgagttccga tcaggctcag cgctttattc
     5161 aacgcacctc tgatgtgatg aaagagctta agtacatcgc cgaaaatcgc ttatcggcag
     5221 aaaacggtgt gctttccccg cgtggacgag aaacgcaggc ggatgtgcct gcgtttgaac
     5281 cgctgtttgc cgactccgat tgttccgcaa tgagtaacac ctggcgaggt tctctggagt
     5341 cattggcgtg gtttatgcgc tactggcgcg ataatttttc tgcggcttac gatctcaacc
     5401 gggtattttt aatcggcagt gataacctct gcatggccaa tttcggtctg cgtgatatgc
     5461 cagtggaacg cgataccgcg ttgaaagcgt tgcatgaacg catcaataaa tatcgaaatg
     5521 caccacaaga tgatagcggc agtaacctct actggatcag cgaaggtccg cgccctggcg
     5581 tcgggtattt ttacgcgttg acgccagttt atctggcgaa ccggttgcag gcgcttttgg
     5641 gtgtcgagca gaccatccgg atggagaact ttttcttacc gggtacgttg ccgatggggg
     5701 ttaccattct tgatgaaaat ggtcataccc tgatttcgct taccggacca gaaagtaaaa
     5761 ttaagggcga tcctcgctgg atgcaggaac gctcttggtt tggctatacg gaagggttcc
     5821 gggagctggt gctgaagaaa aatctgccac cctcatcgct aagcatcgtg tattcggtgc
     5881 cggttgataa ggtgctggaa cgcattcgca tgttgatcct taacgcaatt ttgctgaatg
     5941 tgcttgccgg agctgcattg tttactctcg cgcggatgta cgagcgacgt attttcattc
     6001 cggcggaaag cgacgccctg cgactggaag aacatgagca gttcaatcgc aagattgtcg
     6061 cctccgcgcc agtgggtatc tgcattttgc gtaccgctga tggcgtcaat attttaagta
     6121 acgaactggc gcatacctat ctcaatatgc ttacgcatga ggaccgccaa cgactgacgc
     6181 aaattatctg tgggcagcag gtcaattttg ttgatgtcct gaccagcaac aataccaatc
     6241 tgcaaatcag cttcgtccat tcgcgctatc gtaatgaaaa cgtggccatt tgtgtgctgg
     6301 tggatgtttc ttcgcgcgtg aagatggaag agtcgttgca ggagatggca caagcagcgg
     6361 aacaggcgag ccagtcaaaa tcgatgttcc ttgccaccgt cagtcatgag ctgcgaacgc
     6421 cgctgtatgg cattatcggt aacctggatc tgttgcaaac caaagagtta ccgaaaggcg
     6481 tcgatcgtct ggtgacggca atgaacaact cttccagcct gttgttgaaa attatcagcg
     6541 atattctcga tttctcgaag attgaatcgg aacagttgaa gatcgaaccg cgtgagtttt
     6601 caccgcgtga agtgatgaac cacatcaccg ccaactattt accgctggtg gtacgcaagc
     6661 agttaggctt gtactgcttt attgaaccgg atgtgccagt ggccttaaat ggcgacccga
     6721 tgcgtttaca gcaggtcatc tccaacctgt tgagtaatgc cataaaattc accgataccg
     6781 gctgtatagt tttgcatgtt cgcgctgatg gcgattatct ctctatccgt gttcgcgata
     6841 ccggcgtggg gattccggcg aaagaagtgg tgcgcttgtt tgatcccttc ttccaggtcg
     6901 gaacgggcgt acagcgaaac ttccagggga ccggtctggg tctggcgatt tgtgaaaaac
     6961 tgatcagcat gatggacggc gatatctcgg tagattcaga accgggaatg ggcagccagt
     7021 ttaccgtgcg tattccgttg tacggcgctc agtacccgca gaaaaaaggc gtggaagggt
     7081 tgagtggtaa acgctgctgg ctggcggtcc gcaatgcgtc gctctgtcag ttcctggaaa
     7141 ccagtttgca gcgcagcggc atcgtcgtta caacatacga agggcaggaa ccgactcccg
     7201 aagatgtgtt aatcactgac gaggtagtga gtaaaaaatg gcagggcaga gcggtagtga
     7261 ccttctgtcg tcgccatatt ggtattccgc tggagaaagc gccaggggag tgggtacaca
     7321 gtgtggctgc tccgcatgag ctaccggcat tgttggcgcg tatttatttg atcgagatgg
     7381 agagcgacga tcctgctaac gctctgccgt cgacggacaa agcggtcagc gataacgacg
     7441 atatgatgat tctggtcgtg gatgatcatc cgattaaccg acgtctgctg gcagatcagt
     7501 tgggatcgtt gggctatcaa tgtaaaaccg cgaatgatgg cgtcgatgcg cttaatgtac
     7561 ttagtaagaa tcatattgat atcgtgctta gcgacgtcaa catgccaaat atggatggtt
     7621 accgcttgac gcaacgcatt cgtcagttgg gactgacgtt gccggtaatc ggagtaactg
     7681 ctaatgcgtt ggctgaagag aagcagcggt gtctggagtc cggtatggac agctgcctct
     7741 cgaagccggt aacgctggat gtgataaaac agacgctgac ggtatatgcc gagagggtca
     7801 ggaaatcgcg ggattcgtag gacggataag gcgtttacgc cgcatccggc attcagtgca
     7861 aatgccagat aagacactga cgcgtcttat ctggcctact ttaatgtaat ctgttgaaat
     7921 aatgggaatc gtaggccgga taaggcgttt acgccgcatc cggcaatcgg tgcaaatgcc
     7981 agataagaca ctaacgcgtc ttatctggcc tacagatgat tagtctttat ctgccggact
     8041 taaggtcaca gaagagagat aattcagcag ggcgatatcg ttctcgacgc ccagcttcat
     8101 catcgcagat ttcttctggc tactgatggt tttaatactg cggttcagct ttttagcgat
     8161 ctcggtcact aggaagcctt ccgcaaacag gcgcagaact tcactctctt ttggcgagag
     8221 acgcttgtca ccgtaaccac cagcactgat tttttccaac aggcgagaaa cgctttccgg
     8281 ggtaaatttc ttccctttct gcagcgcggc gagagctttc ggcagatcgg tcggtgcacc
     8341 ttgtttcagc acgatccctt cgatatccag atccaatacc gcactaagaa tcgccgggtt
     8401 gttgttcata gtcagaacaa tgatcgacag gcttgggaaa tggcgcttga tgtacttgat
     8461 taaggtaatg ccatcgccgt acttatcgcc aggcatggag agatcggtaa tcaacacatg
     8521 cgcatccagt ttcggcaggt tgttgatcag tgctgtagag tcttcaaatt cgccgacaac
     8581 attcacccac tcaatttgct caagtgattt gcgaataccg aacaagacta tcggatggtc
     8641 atcggcaata attacgttca tattgttcat gtaataggct accttgctac agcaagctct
     8701 tgacataact gtcaatgtcg ctgatgtatt tttctattcc tggaacatcc ttctcacgaa
     8761 tcagatgttc cagcgtttca cataactgct tgccgggtac cagatttagc atggcaaata
     8821 cgcctttaag acgatgagcc gtttgggcta acgcagcaaa gtcactggtt gctgcttcag
     8881 tatacagcct cttaacatca tccggtactg tgtctacaaa gagcgcataa tagccgctgg
     8941 catggagttg cgcattttca tctccgccca gaggcgattc tgtcacctct tcctgcgcca
     9001 gttgcacttc aattaattgt aagaccgctt cctgcatagc gttgctcata ttgaagttga
     9061 cgcacaattg accaggccca atttcccgta cgtcagactc atcatcgctt aaaagcaagc
     9121 cagaggcagt aagattagac ggattatccg ttaaaaagat atcataatct tgactaatta
     9181 atctttcatc gggtgtgata caggttgcac cccaattttc taacaggcga gtgacaatat
     9241 tccgaatttc tgccgaagta acatccacca ttacgcagac atcatccagt aaacgctctt
     9301 cttcctcttc aacttccggg tcagcggcga gcattttgat atgcacagag tagcgtgtac
     9361 caagcccatc ccgcgttttg atgtttaaat gaccgcccag tttacgtgcc agttgatcgc
     9421 tcagccagaa tgccagcggg tccgcctttc cataacgatc gttttgggtc tggttgataa
     9481 acgggaagtg caaattatcc atttcatgaa tacttacgcc ttctcccgtg tccagaatgc
     9541 ggaatgtcag gcggtcttca gaggactcat cctgatcaac ctcaagggtg atttttccca
     9601 attgcgttga ggtcacggca tattgcatca gtagcagcaa aatacgtcgt aaggcatcgc
     9661 gatcgccgcg gcgcatatcg tgtgctttca gatgattgtt aatcagcagt tgcagacctt
     9721 tacgcttgat ggcaggcaac actgaaggca caacttcatc aattaaatcc tgcacggaga
     9781 acagcaccgt ctcacttttc cagctatcat ccgcaagcat gttcgctaac tgtatttctt
     9841 cgaccagccg caccagcaca tctgcctgat tcgccagttg tttgctttcc ggggcgttga
     9901 gtttagccgc actctccgcc agggactgtg cgggttcttt cagcgcatcg ccaatgtttt
     9961 tcataaaggt catccgcccc tgctggtttt tctcatacag acgctgcgcc tgcttgagtt
    10021 tcttgtttac cagcacttcg cgatcctgat cgcgaataat gaaaatttgt gtgcgcggcg
    10081 cgacctggct gcggaacatg cggatctcat acagctcgtt attgatcgtc gcctgaataa
    10141 tcccctgatg ctgttccgcc atggtggtga tgttttgcag attcagatgc ggcagcaaat
    10201 gatcggcaat tttgttactt atgacagtgc ggttcgattc ctgatcgtga accagcaggc
    10261 cgagcggcaa cagtgagact atctcttcat tgattgcccg taaaatgcgc aattcgttat
    10321 tgaccgccgt gctggggacg ctttctgtac tgcggctgga gaaatggcgg aatgtggtat
    10381 agccaaataa cgccagcgcc agcaaaccga tgttcagcag cagtggcagc agaatgtttt
    10441 gcaacgtatc cagcaataag gtgccataag gaacctgcca gaccaggcgc atatcggtag
    10501 agttgagtgc cgaggagatt tcaatcttcg tactgttaaa gtggatactg acgctatccg
    10561 tcccttcttt ctcattatca ttgtttcccg ttgccgtcgc gtctggctca aggcggaaac
    10621 tgtccagcgg catacccggt gggatcaaat cattaatcgg cagatcaaaa gccacgaccg
    10681 ttgccagatg tcccggctgg ttgaacgtgg tacgcaaggt aaagtaatga ccgttctgcc
    10741 aggccaggcg gcgcatgtta gaaaaacttt cgcgttcatc gagggcgttg gcctgttgca
    10801 acatttctgc acgacgtgaa tcaacaatgt cactgacggt cgattcttta aatccggagg
    10861 tgagatcttt aaggggtagg gttgagatca gcaccagact gttatcctga ccattcaggt
    10921 aatacatcga ccacggtaca ttttctgcac cccacaatgt atccagataa gtggacatcc
    10981 gcagagtcat ctcaagcgtt gagttgtcgt gagagccaaa gattaacgct tccgttttgc
    11041 ggcgtggttt ctccagatag tagacatcct gtttcaggcg cgtctcttgt aaaccttcgc
    11101 cggaggaggg ggaggtcgtc gcggcaatgt tgtcgtagat ctgccaggtc acgtaacgcc
    11161 aggtatcgac gcgcttttgg attgcgtggg taatgtcgac aatctggtaa cttttatctt
    11221 tcagccaggc gttaacggca ctttgtacca ttacccccat cgtcaccagt aacacaatga
    11281 tcaacagtaa aaagaagcgg gtaatgctcc ccggtaggag tgaaaagcgg gtcgtggccg
    11341 ttgtctcttt ctgacgcatt cgtgtttatg acctgttaaa acttcgcgaa gtgatagggc
    11401 agtataaagg gtacagtgtg aaattccaga ctcttacctg attgatgatt ctctattctc
    11461 ccgtgatatt tatcatcctg tatgggggga aatagatgta caagcgccat tttgctgtac
    11521 aaataaccta caaaaagccc aacgaaatta attgcaaata aacaaagatt gctggaaatt
    11581 atgctgatgt taattatttg tgaaatagtt aacaagcgtt atagtttttc tgtggtagca
    11641 cagaataatg aaaagtgtgt aaagaagggt aaaaaaaaac gaatgcgagg catccggttg
    11701 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    11761 agcgggagtt attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    11821 cttaagaata agttattgat tttaaccttg aattattatt gcttgatgtt aggtgcttat
    11881 ttcgccattc cgcaataatc ttaaaaagtt cacttgtatt tacattttga aacatctata
    11941 gcgataaatg aaacatctta aaagttttag tatcatattc gtgttggatt attctgcatt
    12001 tttggggaga atggacttgc cgactgatta atgagggtta atcagtatgc agtggcataa
    12061 aaaagcaaat aaaggcatat aacagagggt taataacatg aaagttaaag tactgtccct
    12121 cctggtccca gctctgctgg tagcaggcgc agcaaacgct gctgaagttt acaacaaaga
    12181 cggcaacaaa ttagatctgt acggtaaagt agacggcctg cactatttct ctgacgacaa
    12241 gtctgtagat ggcgaccaga cctacatgcg tcttggcttc aaaggtgaaa ctcaggttac
    12301 tgaccagctg accggttacg gccagtggga atatcagatc cagggcaaca gcgctgaaaa
    12361 cgaaaacaac tcctggaccc gtgtggcatt cgcaggtctg aaattccagg atgtaggttc
    12421 tttcgactac ggtcgtaact acggcgtagt ttacgacgta acttcctgga ccgacgtact
    12481 gccagaattc ggtggtgaca cttacggttc tgacaacttc atgcagcagc gtggtaacgg
    12541 cttcgcaacc taccgtagca ccgacttctt tggtctggtt gacggtctga acttcgctgt
    12601 tcagtatcag ggcaaaaacg gtagcccgga aggtgaaggc atgaccaaca acggtcgtga
    12661 agctctgcgt cagaacggtg acggcgttgg cggttctatt acttatgatt acgaaggctt
    12721 cggtatcggt gctgcagttt ccagctccaa acgtactgat gaccagaact tcggtctgaa
    12781 ccgttatgat gagcgttaca tcggtaatgg cgaccgcgct gaaacctaca ccggtggtct
    12841 gaaatacgac gcgaacaaca tctacctagc tgctcagtac acccagacct acaacgcaac
    12901 tcgcgtaggc aacttgggtt gggcgaacaa agcacagaac ttcgaagctg ttgctcagta
    12961 ccagttcgac ttcggtctgc gtccgtccct ggcttacctg cagtctaaag gtaaaaacct
    13021 gggtgtcatc aatggtcgta actacgacga cgaagatatc ctgaaatatg ttgatgttgg
    13081 tgcgacctac tacttcaaca aaaacatgtc cacctacgtt gactacaaaa tcaacctgct
    13141 ggacgacaac cagttcactc gtgacgctgg catcaacact gataacatcg tagctttggg
    13201 tctggtttac cagttctaat ctcgatggat atcgaacaaa gggcctgcgg gccctttttt
    13261 cattgttttc agcgtacaaa cacagttttt tggtgtactc ttgcgaccgt tcgcatgagg
    13321 ataatcacgt atggaaataa gctttacccg cgtggcactg ctggttgccg cgctcttctt
    13381 tgttggttgc gatcaaaaac cacaacccgc caaaacccac gctactgaag ttactgttct
    13441 tgaaggcaaa accatgggta ccttctggcg tgccagcatc ccgggcattg atgccaaacg
    13501 cagtgccgaa cttaaagaaa agattcagac ccagctggac gccgacgatc agctgctttc
    13561 gacctataaa aaagattccg cgctgatgcg ctttaacgac tcgcaaagtt tgtcgccgtg
    13621 gccggtaagt gaagcgatgg ccgatatcgt caccacctcg ctgcgcattg gggcaaggac
    13681 cgatggtgcg atggatatta ccgtcgggcc gctggtgaat ctgtggggct ttggcccgga
    13741 acaacagccg gttcaaattc cgagccagga acagatcgat gcgatgaaag ccaaaaccgg
    13801 cttacagcac ttgacggtca tcaatcagtc gcatcagcaa tatctgcaaa aagacctgcc
    13861 ggatttatat gtcgatctct ccaccgtcgg tgaaggttat gcggcggatc acctggcacg
    13921 cttgatggag caggaaggga tttcccgcta tctggtgtcg gtgggcggcg cgctgaacag
    13981 ccgtggtatg aacggtgaag gccagccgtg gcgggtagcg attcaaaaac caaccgataa
    14041 agaaaacgcg gttcaggctg tggtggatat caacggtcat gggattagca cctctggcag
    14101 ctaccgtaac tattacgaac tggacggcaa acgtctttcc catgttatcg atccgcaaac
    14161 cgggcgtccc atcgaacaca atctggtatc cgtgacggtg attgctccga cggcgctgga
    14221 agccgatgcc tgggatactg gcttgatggt actcgggccg gagaaagcca aagaggttgt
    14281 tcgccgggaa gggctggcgg tttatatgat caccaaagaa ggcgatagct ttaaaacctg
    14341 gatgtcacca cagtttaaaa gcttccttgt cagcgaaaaa aattaaagcg caagattgtt
    14401 ggtttttgcg tgatggtgac cgggcagact aaaggctatc cttaaccagg gagctgatta
    14461 tgaaaaacgc cacatgctta actgacgatc aacgctggca atctgtctta gcccgcgacc
    14521 cgaatgccga cggcgaattc gttttcgccg tgcgcaccac gggtatcttt tgccgtccgt
    14581 cttgccgcgc cagacatgcc ttgcgggaaa atgtctcctt ctacgcaaat gccagcgagg
    14641 cactcgccgc cggatttcgc ccctgcaaac gttgtcagcc agacaaagcc aatccccggc
    14701 aacatcggtt ggataaaatc acccacgcgt gtcgactgct ggaacaggaa acgcctgtaa
    14761 cgctggaagc cttagccgac caggtggcga tgagtccatt tcatctacat cggttgttta
    14821 aagcgactac cggaatgacg cctaaagcct ggcaacaggc ctggcgcgct cgccgtttgc
    14881 gcgaatcgct ggcgaaaggg gagagcgtga cgacgtctat tcttaacgcc ggattccccg
    14941 acagcagcag ttactaccgc aaagccgatg aaacgctggg catgacggct aaacaattcc
    15001 gtcacggtgg cgaaaatctg gcggtgcgtt acgcgctggc tgattgtgag ctgggtcgtt
    15061 gcctggtggc agaaagcgag cgggggattt gcgcgatatt gctgggcgat gatgacgcga
    15121 cattaatcag cgagttgcag cagatgtttc ccgctgccga caacgcgcct gccgatctga
    15181 cgtttcagca acatgtgcgt gaagtgatcg ccagcctcaa tcaacgcgat acgccgctga
    15241 cgttaccgct ggatattcgc ggcactgctt ttcagcaaca agtctggcag gcactgcgca
    15301 cgataccttg tggtgaaacc gtcagttatc agcaactggc taacgccatc ggcaaaccga
    15361 aagcggtacg ggccgttgcc agcgcctgtg ccgccaacaa gctggctatc gtaataccct
    15421 gtcatcgagt ggtccgtggt gatggcacac tttccggtta ccgctggggc gtgtcgcgta
    15481 aagcgcaact gctgcgccgc gaagctgaaa atgaggagag gtaatgttgg atctgtttgc
    15541 cgatgctgaa ccgtggcaag agccactggc ggctggtgcg gtaattttac ggcgttttgc
    15601 ttttaatgct gcggagcaac taatccgcga tattaatgac gttgccagcc agtcgccgtt
    15661 tcgccagatg gtcgctccag gcggatatac catgtcggtg gcgatgacca actgtgggca
    15721 tctgggctgg acgacccatc ggcaaggtta tctctattcg cccattgatc cgcaaacaaa
    15781 taaaccgtgg cccgccatgc cacaaagctt tcatgattta tgtcaacgtg cggctacggc
    15841 ggcgggctat ccagatttcc agccagatgc ttgtcttatc aaccgctacg ctcctggcgc
    15901 gaaactgtcg ctgcatcagg ataaagacga accggatctg cgcgcgccaa ttgtttctgt
    15961 ttctctgggc ttacccgcga tttttcaatt tggtggcctg aaacgtaatg atccgctcaa
    16021 acgtttgttg ttggaacatg gcgatgtggt ggtatggggc ggtgaatcgc ggctgtttta
    16081 tcacggtatt caaccgttga aagcggggtt tcatccactc accaccgact gccgctacaa
    16141 cctgacattc cgtcaggcag gtaaaaaaga ataaaaataa gaattattat tgctgtgcgc
    16201 gcgaagatgt ttaaactgcg ggctgtaatt cattgtccgg gttttctgca tggaacttct
    16261 tgtacttgtc tggcggcagt atcgctggcc atttatcagt gtgatggcgc taagcctcgc
    16321 cagtgcggca ttaggcattg gcttaattgc ttttatcaat cagcgcctta tcgaaacggc
    16381 ggataccagt ctgctggtgt taccggagtt tctgggatta ttgctgctgt tgatggcagt
    16441 cactctcgga tcgcaactgg cgctcaccac tttggggcat cacttcgttt accgactgcg
    16501 tagtgaattt atcaagcgga ttctggatac ccacgtcgag cgcattgaac aactcggtag
    16561 cgcctcgttg ctggcgggat taaccagcga tgtgcgcaat atcaccattg cttttgtgcg
    16621 tctgccggaa ctggggcagg ggatcattct cactatcggt tcatcggcgt atctgtggat
    16681 gctgtcgggc aaaatgttgc tggtaacggc tatctggatg gcgatcacca tctggggcgg
    16741 ttttgtgctg gtggcgcggg tgtacaaaca tatggcgacc ctacgtgaaa ccgaagacaa
    16801 gctgtacacg gattttcaaa ctgtacttga agggcgcaaa gagctgactc tgaaccggga
    16861 acgcgccgag tatgtgttta acaacctcta catccccgat gcgcaagagt atcgccatca
    16921 tattattcgc gcagacacct tccatcttag tgccgtgaac tggtcaaaca tcatgatgct
    16981 gggcgctatc ggcctggtgt tctggatggc gaacagtctc ggttgggctg ataccaacgt
    17041 tgctgcgacc tattcgttga cgctgttatt cctgcgtacg ccgctgcttt cggcggttgg
    17101 cgcattgccg acgctgctga cggcgcaggt ggcgtttaac aagctgaaca aattcgcgct
    17161 cgcgcctttc aaagctgagt ttccgcgccc gcaggcgttt cccaactggc aaacgctgga
    17221 gctgcgtaac gtgacgtttg cttatcagga taacgcgttt tccgttggtc cgattaatct
    17281 caccatcaaa cgtggcgagc tgctgtttct gattggcggc aacggtagcg gaaaatcgac
    17341 gctggcgatg ttgttgacgg gcttgtatca gccacaaagc ggcgaaatct tgctggatgg
    17401 caaacctgtc agcggcgaac aaccggaaga ttatcgcaaa ctgttttcgg cagtgtttac
    17461 cgatgtctgg ctgtttgatc aactgctggg gccggagggg caacccgcta acccgcaact
    17521 ggttgagaag tggctggcgc agctgaaaat ggctcataag cttgagttaa gcaacgggcg
    17581 tattgttaac ctgaagttat caaaagggca gaaaaaacgc gtggcgctgt tgctggcgct
    17641 ggcagaagaa cgcgatatta tcctgctgga tgaatgggcg gcggatcagg atccacactt
    17701 ccgtcgtgag ttttatcagg tgttgctgcc gctgatgcag gagatgggta aaactatttt
    17761 cgctatcagt catgatgatc attactttat ccacgccgac cgcctgctgg aaatgcgcaa
    17821 tgggcaactt agcgagctga cgggcgaaga gcgcgatgcc gcttcgcgtg atgccgttgc
    17881 ccggacggca taatattacg acgctgacct ggcggactgc tgccgtcagg tcaatatgct
    17941 aaaaatccct tcatttctaa taaccctata attaatcaac gaaattataa tgtttctaaa
    18001 attagaatat aatttataaa cattatttaa atgttgttac ttaagtgtta ccgttgatgc
    18061 cgcgcaaatc tcacctgcaa taaagcgact aaaagtaagg cattaacaag atgaaaaaag
    18121 tgactgccat gctcttctcg atggccgtgg ggcttaatgc cgtttcgatg gcggcaaaag
    18181 cgaaagcgtc cgaggagcag gaaactgatg tactgttgat tggcggcggc attatgagcg
    18241 ccacgttggg gacctattta cgcgagctgg agcctgaatg gtcgatgacc atggtggagc
    18301 gcctggaggg gggcgcgcag gagagttcga acggctggaa caacgccgga accgggcatt
    18361 ctgcactgat ggaactgaac tacaccccgc aaaacgccga tggcagcatc agtattgaaa
    18421 aagcagtcgc cattaacgaa gcatttcaga tttcccgcca gttctgggcg caccaggtcg
    18481 agcgcggcgt gctgcgtact ccgcgttcat ttatcaatac cgttccgcat atgagctttg
    18541 tctggggcga ggataacgtt aatttcctgc gcgcccgtta cgccgcgttg caacaaagct
    18601 cgctgtttcg cggtatgcgt tactctgaag atcacgcgca gatcaaagag tgggcaccgt
    18661 tagtgatgga agggcgcgat ccgcaacaga aagtagcggc aacacgcaca gaaattggca
    18721 ctgatgtgaa ctatggtgag atcacccgcc agttaattgc ttccttacag aagaaatcta
    18781 acttctcgct gcaactcagc agcgaagtcc gcgccctaaa gcgtaatgac gataacacct
    18841 ggaccgttac cgttgccgat ctgaaaaacg gcactgcaca gaacatccgc gccaaatttg
    18901 tctttatcgg cgcgggcggt gcggcgctga agctgttaca ggaatcgggg attccggaag
    18961 cgaaagacta cgccggtttc ccggtgggcg gacagttcct tgtttcggaa aatccagacg
    19021 tggttaatcg ccatctggcg aaagtttacg gtaaagcatc cgttggcgca ccaccgatgt
    19081 cggttccgca tatcgatacc cgtgttctgg acggtaaacg cgtagtgctg tttgggccat
    19141 ttgccacctt ctcaaccaaa ttcctcaaaa acggttcatt gtgggatctg atgagttcca
    19201 ccaccacctc taacgtgatg ccgatgatgc acgtcgggct ggataatttc gatctggtga
    19261 aatatctggt gagtcaggtg atgttgagtg aagaggatcg ttttgaagcg ttgaaagagt
    19321 actatccgca agcgaaaaaa gaggactggc gtttgtggca agcggggcag cgtgtgcaga
    19381 ttatcaagcg tgatgccgag aaaggtggcg tactgcgtct gggtaccgaa gtggtcagtg
    19441 accagcaagg gaccattgcg gcgctgctgg gggcatcgcc aggggcgtca accgccgcgc
    19501 cgattatgtt gaatctgctg gaaaaagtat ttggcgatcg cgtttccagc ccgcaatggc
    19561 aggctacgtt gaaagcgatc gttccgtctt atggacgcaa gctgaacggt gatgtagcgg
    19621 caacagaacg cgagttgcag tacaccagcg aagtgctggg gctgaaatac gacaaaccgc
    19681 aagcagcaga tagtacgccg aaaccgcagt tgaaaccgca acccgttcaa aaagaagtgg
    19741 cggatattgc gttgtaatga tgtgccacat ccggcatggt atgccggatg tggcgtatac
    19801 tgataagacg cgccagcgtc gcatcaggca accggctcgg gcgttagatg ttggatgcgg
    19861 cgtaaccgcc ttatccaacc aacggggtgc cgtacttcac aacagtttag cgaacttccg
    19921 cgttgtcaat tttctcttcc gccttccaga tgcggtattt cacatctaca ttgtctggcg
    19981 tatacaccac gatcggcagc ttgctgttgt aacgcagcat tccagcatcg cccagatacg
    20041 cggtgacaaa tttcttctct ttcttgccat ccgggcaggc catcatcgtg gaaaccggcg
    20101 agctaacttt atcaaagaca taataatcat agccccagcc ttccagcgtt ttgctttcca
    20161 gcttcccgcc gagacgatgc aaattgcaat cgacttccag cgtctgaccg attaacagtt
    20221 ctactttcag ggtagattca tcttcttgcg gggttaactg aatcacctga cgcttcatgc
    20281 ctgtttcagc ttgtggataa ggcgcgattt tttccagtgg ctggacgctt tctgccgccc
    20341 aggcggaagt ggtagcgaaa gcggcaaaca atactgcagg tagaatggtc ttcatttttt
    20401 tgctttcctt cttcatttat aggttttcac cgatgatgtc gctgaaggtt aacatattca
    20461 tcagatttat taatttgctt tatgaataac ttaaagaaca ttcagacaat tttacctgct
    20521 cttttagtgt taaattctga tgagggagca aaccagcaga aatatctctg gtgaatataa
    20581 tcgctatata aatatgttta taaccatttg aaatgtgagc aaaagcccgt ttttgccacg
    20641 cttcccgcca ctcttttgat cctgctacag gttttacccc gatcggggta tgcatctttg
    20701 acacatcctt taatatctta gcggctataa aaatggctta ttaattatgc ggtctatttg
    20761 gtcgctctca attttcagag cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc
    20821 ccgtcggggc atactcactg gtcgctggcg caaagccagt aacggtatcc gtccgccctg
    20881 gtccggtgat gaatctcatt ttctgaccca ttgcacccgc tgcgacgcct gtattaatgc
    20941 ttgtgaaaac aacattctgc aacgcggcac gggtggctat ccgagcgtta atttcaaaaa
    21001 taacgagtgc agcttctgtt acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc
    21061 gcgccacacc agggcctggg atttgcagtt caccatcggg gacgcgtgcc tggcgtatca
    21121 gtcagttgaa tgccgccgct gtcaggatag ctgtgagcca atggcaatta tctttcgccc
    21181 gacgctctcc gggatctacc agccgcaact taatagccaa ctttgcaacg gatgtggagc
    21241 atgtgcagcc agctgtccgg tatcagccat aactgcggag tatctccatg cacactaact
    21301 ggcaagtttg cagcctggtc gtgcaggcca aaagcgaacg aatttcagac atcagcaccc
    21361 aacagaacgc ctttcccggc tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga
    21421 ttgtggtggt ggaagcagaa gacagcgaaa cgctgatcca aaccattgag tcagtacgca
    21481 acgtagaggg cgtgctggcg gtgtcgctgg tttatcacca gcaggaagag caaggtgagg
    21541 aaacaccatg aaactcagtc gtcgtagctt tatgaaagct aacgccgttg cggccgctgc
    21601 ggcggctgcc ggtctcagcg tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc
    21661 cattaaatgg gataaagcgc cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg
    21721 aacgcagcag gggcgtgtgg tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg
    21781 cctgaactgc attaagggct atttcctgcc caagatcatg tacggtaaag accgtttgac
    21841 gcagccgctg ctgcgtatga aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat
    21901 cacctgggat caggctttcg atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa
    21961 agggccggaa tcgatcggta tgttcggttc tggtcagtgg actatctggg aaggttatgc
    22021 cgcgtccaag ctgttcaaag cgggcttccg ttcgaacaac atcgacccga acgcacgtca
    22081 ctgtatggcg tcggcagtag ttggctttat gcgtaccttt ggtatggatg agccgatggg
    22141 ctgctatgac gacatcgagc aggctgacgc gtttgtgctg tggggcgcta acatggcgga
    22201 gatgcacccg atcctctggt cacgcattac taaccgtcgt ctctctaacc agaacgtcac
    22261 cgtggcggtg ctttctacct accagcatcg tagcttcgag ctggcggata acggcatcat
    22321 ctttacgccg caatctgacc tggtgatcct gaactacatc gccaactata tcattcaaaa
    22381 caatgcgata aatcaggact tcttcagcaa gcacgttaac ctgcgcaaag gggcgacgga
    22441 catcggctac ggtttacgtc cgacccatcc gctggaaaaa gcagcgaaga atccgggttc
    22501 tgacgcctcc gaaccgatga gctttgaaga ttacaaagcc ttcgttgctg agtatacgct
    22561 ggaaaaaacc gctgaaatga ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct
    22621 gtatgccgat ccgaacaaga aagtcatctc ctactggacg atgggcttca accagcatac
    22681 ccgtggtgtg tgggccaaca acctggtcta caacctgcac ctgctgaccg gcaaaatttc
    22741 ccagccgggt tgcggtccgt tctccctgac cgggcaacct tctgcgtgtg gtactgcgcg
    22801 tgaagtgggc acctttgctc accgtctgcc tgcggacatg gtggtaacta acgagaaaca
    22861 tcgtgatatc tgtgagaaga agtggaatat cccgagcggc accattccgg cgaaaatcgg
    22921 tcttcatgcg gtggcacaag accgtgcgct gaaagacggc aagctgaatg tttactggac
    22981 catgtgtacc aacaacatgc aggccgggcc gaacatcaat gaagagcgta tgccgggctg
    23041 gcgcgatccg cgcaacttca tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc
    23101 cgccgacttg atcctgccga ccgcaatgtg ggtagagaaa gagggcgctt acggtaacgc
    23161 cgaacgccgt actcagttct ggcgtcagca ggtacaggcg ccaggcgaag cgaaatcgga
    23221 tctctggcag ttagtccagt tctcccgccg cttcaaaact gaagaagtat ggccggaaga
    23281 gctgctggcg aagaaaccgg aactgcgtgg caaaacgctg tacgaagttc tgtatgccac
    23341 ccccgaagtg agcaaattcc cggtatccga actggcggaa gatcagctga acgatgaatc
    23401 ccgcgagctg ggcttctatc tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg
    23461 cggtcacggt cacgatctgg caccgttcga tgactaccac aaagcgcgcg gtctgcgctg
    23521 gccggtggtg aatggtaaag aaacacagtg gcgttacagc gaaggtaacg acccgtacgt
    23581 gaaagcgggc gaaggctata agttctacgg taaaccggat ggcaaagcgg tgatcttcgc
    23641 actgccgttc gaaccggcgg cggaagcacc ggatgaagag tacgacctgt ggctctctac
    23701 cggacgcgtt ctggagcact ggcacaccgg cagtatgact cgccgtgtgc cggaactgca
    23761 ccgcgccttc ccggaagcgg tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct
    23821 gcgccgtggc gacaaagtga aagtggtttc tcgccgtggc gaagtgatct cgattgttga
    23881 aacgcgcggt cgtaaccgtc cgccacaggg cctggtgtac atgccgttct tcgacgccgc
    23941 acagctggtt aacaaactga cgctggatgc gaccgatccg ctctcgaaag agacggactt
    24001 caagaagtgc gcggtcaaac tggagaaggt gtaagccatt atgtcccggt cagcgaaacc
    24061 tcaaaatggt cgccgccgct ttctgcgcga tgtcgttcgc acagcaggcg ggctggctgc
    24121 cgtgggtgtg gcgctggggt tacaacagca aaccgcacgc gcatctggcg tgcggttgcg
    24181 cccgcccgga gccataaacg agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg
    24241 tgttcaggct tgcccttacg acaccttaaa actggcgacg ctggcctctg gtctgtcggc
    24301 gggcacgcca tattttgtcg cacgggatat tccttgcgaa atgtgtgagg acattccgtg
    24361 cgccaaagtg tgcccaagcg gtgcgctgga tcgtgagatt gaatcgatcg acgacgcgcg
    24421 gatggggctg gcggtactgg tggaccagga aaactgtctc aactttcagg ggctgcgctg
    24481 cgatgtttgt tatcgcgaat gcccgaaaat tgatgaggcc atcaccttgg agctggagcg
    24541 caacacgcgt accggtaagc acgcccgctt tctgccgacg gttcacagcg acgcctgtac
    24601 cggttgcggt aagtgcgaaa aagtatgcgt gctggaacaa ccggcaatca aggtgttacc
    24661 gctgtcactg gcgaaagggg agttaggtca ccattaccgc ttcggctggc tggaggggaa
    24721 caatggcaaa tcgtaaacgt gacgccgggc gcgaggcgct ggagaaaaaa ggctggtggc
    24781 gcagtcaccg ttggctggtg ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt
    24841 tgagcggtcc atggtttggt gtgtggatct tgcacggcaa ctacagcagt agcctgttat
    24901 tcgacaccgt tccgctgacc gatccgctga tgacgctgca aagtcttgcc agtggtcatc
    24961 tgcctgccac ggtggcgttg accggtgcgg tgattatcac cgtgctctac gccctggcgg
    25021 gaaagcgatt attttgcagc tgggtctgcc cgctgaaccc gattaccgac ttagcgaact
    25081 ggttacgcag gcgctttgac ctcaatcagt ctgcgacgat ccctcgccac attcgctacg
    25141 tgctgttggt ggtcattctg gtgggatcag ccttaactgg cacgctcatt tgggaatgga
    25201 tcaaccccgt ttctctgatg gggcgtagcc tggtaatggg cttcggcagc ggcgcgctgc
    25261 ttattctcgc gctgttttta tttgatctac tggtcgttga acacggctgg tgcgggcaca
    25321 tttgtccggt tggcgcgctg tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg
    25381 ccagcgatcg gcagaaatgt aaccgctgta tggattgttt tcatgtttgc ccggaaccgc
    25441 atgtgctacg tgccccggtg ctggatgaac aaagcccggt gcaggtcacc agccgcgatt
    25501 gcatgacgtg cggtcgctgc gtggatgtct gttctgagga tgtatttaca ataactacac
    25561 gatggagttc gggagcgaaa tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg
    25621 gacggcgatg ctggccctgg tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt
    25681 tagccagtcg ccggaagtat ccgggacgca ggaaggggcc attcggatgc caaaagagca
    25741 ggatcggatg ccgctgaact atgtgaatca gccgccaatg atcccgcata gcgttgaagg
    25801 ttatcaggta acgaccaata ccaatcgctg cttgcagtgc cacggtgtcg aaagctatcg
    25861 caccactggc gcgccgcgta tcagtcctac tcactttatg gatagcgacg gcaaagtggg
    25921 cgcggaagtg gctccgcgtc gttatttctg tctgcaatgt cacgtaccgc aggccgatac
    25981 cgcgccaatc gtggggaata cctttacccc atcaaaaggt tacgggaaat aagaggttat
    26041 tatgggaaat tctgaccgta agcctggtct gattaagcgc ctgtggaaat ggtggcgtac
    26101 ccccagccat ctggcgctgg ggacgctgct gttgatcggt tttgttggcg gcatcgtctt
    26161 ctgggggggc tttaacaccg ggatggaaaa agccaatacc gaagagttct gcattagctg
    26221 ccacgaaatg cgcaacacgg tgtatcagga atacatggat tccgtgcact acaacaaccg
    26281 tagcggcgtc cgtgcgacct gtccggattg tcacgttccg cacgagtttg tgccgaagat
    26341 gatacgcaag ctcaaagcaa gtaaagagct gtatggtaaa atttttggcg ttattgacac
    26401 gccgcagaaa tttgaagctc atcgtctgac gatggcacag aatgagtggc ggcgcatgaa
    26461 ggacaataac tcgcaggagt gccgtaactg tcacaacttc gagtatatgg atacaaccgc
    26521 ccagaaatcg gttgccgcga agatgcatga ccaggcggtg aaagatgggc aaacctgtat
    26581 tgattgccat aaagggatcg cgcacaagct gcccgatatg cgtgaagtcg agctaggttt
    26641 ttaacagggt tattgcgtgg gtatgcttga agccagagag ttactttgtg agcgggatga
    26701 acgaacctta tttagtggct tgtcatttac gctgaacgca ggagagtggg tacaaatcac
    26761 cggtagcaac ggcgcgggga agacaacgct tctccgtttg ctgacggggt tgtctcgccc
    26821 tgacgctggc gaggttctct ggcaagggca gcccttgcat caggtacgcg acagctacca
    26881 tcaaaacctg ttatggatag gccatcagcc ggggatcaaa acccggctga cggcgttaga
    26941 aaatctgcac ttttatcatc gcgatggcga tgccgcacaa tgtctggaag ccctggcgca
    27001 ggccgggctt gccggattcg aagatattcc ggtaaatcaa ctttctgccg ggcaacaacg
    27061 ccgcgtcgct ttagcgcgtc tgtggttgac ccgtgccaca ttatggatcc tcgacgagcc
    27121 ttttaccgcg attgacgtta acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac
    27181 ggagcagggg gggattgtga ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa
    27241 aattcgccgc atttcactga cgcaaacgag ggccgcatga tgttctggcg tattttccgt
    27301 cttgaactgc gcgtagcgtt tcgccatagc gccgaaatcg ccaacccgct gtggttcttc
    27361 ctgattgtaa ttaccctttt tccgctcagt atcggtccgg agccgcaact gctggcgcgt
    27421 attgcaccgg gcattatctg ggttgctgcg ctgctttcat ccttgctggc gctggaacga
    27481 ctgttccgtg acgatttgca ggacggcagt cttgaacaat tgatgttgtt gccgttaccc
    27541 ttgcccgccg ttgtgctggc gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta
    27601 ctcatccttt cgccactggt agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg
    27661 atggcgctga cgctgctgct gggaacgcct acgcttggct ttctcggtgc accgggcgtg
    27721 gcgctgacag tgggacttaa gcgcggtggt gtgctgctca gcatactggt gttaccgctg
    27781 actatcccat tactcatctt tgccaccgcc gcgatggacg cggcttctat gcatttgccc
    27841 gttgatgggt atctggcaat tttaggcgcg ttgctggcag gcaccgcgac attaagtcct
    27901 tttgcgacgg cggcagcgtt acgaatcagc attcaataac gctggattac tgatttttgt
    27961 tcgagtctag tatcgaaact atgtggaaaa cactgcatca actggcgatc ccaccacggc
    28021 tgtatcaaat ctgtggctgg tttataccgt ggctggcaat tgccagtgtg gtcgtgctta
    28081 ccgtcggctg gatctgggga ttcggctttg ctccggctga ttatcagcag ggaaatagct
    28141 accgcattat ctacctgcat gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa
    28201 tggcggtggc ggcgtttatt ggccttgtct ggcagatgaa aatggccaac ctggcggtgg
    28261 cggcgatggc ccccattggt gccgtgttta cctttattgc cctggttacc ggctctgcat
    28321 ggggaaaacc gatgtggggc acctggtggg tatgggatgc acgtctgact tctgaactgg
    28381 tgctgctgtt tttgtatgtg ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc
    28441 tggcgggccg tgcggcaggt atcctggtgc tgattggcgt ggtgaatcta ccgattattc
    28501 attactccgt ggagtggtgg aacaccctgc atcagggatc aacgcggatg cagcaaagta
    28561 tcgatccggc gatgcgttcg ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg
    28621 ccacgctgac gctgatgcgg atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt
    28681 gggtgagtga actgatactg aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa
    28741 tgaatttttc gcaatgggcg gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt
    28801 tattccgctg gtggttttgg tcgtgcactc ggtgatgcaa catcgcgcaa ttctgcgtgg
    28861 tgtggcgcaa cagcgggcgc gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc
    28921 atgaatattc gccgtaaaaa ccgcttgtgg attgcctgtg ccgtgttggc agggctggcg
    28981 ctgactatcg gtctggtgct atatgcgctg cgctcgaata tcgatctctt ttatacgccg
    29041 ggggaaattc tctacggcaa gcgtgaaact cagcagatgc cggaagtcgg tcagcgtctg
    29101 cgcgttggcg ggatggtgat gccgggtagc gtgcagcgcg atcccaattc gctgaaagtg
    29161 accttcacca tttacgatgc tgaaggctca gtggatgtct cttacgaagg cattttgccg
    29221 gatctgttcc gtgaagggca gggcgttgtg gtgcagggcg agctggaaaa aggcaatcat
    29281 atcctcgcga aagaagtgct ggcgaaacac gacgaaaact acacgccgcc agaagttgag
    29341 aaagcgatgg aagctaacca ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga
    29401 tgccggaaat tggaaacggg ctgctgtgct tggcgttagg gattgcgctg ctgctgtccg
    29461 tgtatccgct atggggcgtg gcgcgcggag atgcgcgcat gatggcgtct tcccgcttgt
    29521 ttgcctggct gctgtttatg tctgtggctg gcgcatttct ggtgctggtc aatgctttcg
    29581 tggtcaacga ctttaccgtc acctatgttg ccagcaactc caatacccag cttccggtgt
    29641 ggtatcgcgt ggcggctacc tggggcgcgc atgaaggttc gcttctgctg tgggtgctgc
    29701 tgatgagcgg ctggaccttt gcggtggcga tttttagtca gcgtattccg ctggatattg
    29761 tggcccgtgt actggcgata atggggatgg tcagcgtcgg ctttttgctg ttcattctct
    29821 ttacctctaa cccgttctca cgcacgctgc cgaacttccc gattgaaggg cgcgatctta
    29881 acccgctgtt acaggatccg gggctgatct tccatccgcc tctgctttat atggggtacg
    29941 tgggtttctc ggtggcgttt gcttttgcca ttgcttcttt gctgagcggg cgtctggaca
    30001 gcacttatgc gcgttttact cgcccgtgga cgctggcggc gtggattttc ctgacgctcg
    30061 gcattgtgct cggttccgca tgggcctatt acgaactcgg ctggggtggc tggtggttct
    30121 gggatccggt agaaaacgcc tcgtttatgc cgtggctggt ggggactgca ctgatgcact
    30181 cactggcggt cactgaacaa cgcgccagct tcaaagcgtg gacattactg ctggcaatca
    30241 gtgccttctc gttgtgtctg ttggggactt tcctcgtgcg ttccggcgtg ctggtatcgg
    30301 tacacgcgtt tgcgtctgat ccggcgcgcg gtatgtttat cctcgccttt atggtgctgg
    30361 tgattggcgg ttcgctgctg ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa
    30421 acaatgcgct gtggtcgcgg gaatctttgc tgttagcgaa caacgttttg ctggtcgccg
    30481 cgatgctggt ggtgttgctg gggacgctgc tgccgctggt gcacaagcaa cttggactgg
    30541 gcagtatttc gattggcgaa ccgttcttca acaccatgtt tacctggctg atggtgccgt
    30601 ttgcgctact gcttggtgtc ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa
    30661 tccgcaattt attgattatc gccttcatct ctacgctggt gctgtctctg ctgttgccgt
    30721 ggctgttcga aagcaaagtt gtggcgatga cggtgctcgg cctggcaatg gcctgctgga
    30781 ttgcggtgct ggcaactgcg gaagctgcgc tgcgtatttc acgcggcacg aaaaccacct
    30841 tcagttattg ggggatggtg gcggctcacc tggggctggc agtgacaatt gttggcattg
    30901 cctttagcca gaattatagc gttgagcgtg atgtgcgcat gaagtccggc gatagcgtcg
    30961 atattcatga atatcgcttc accttccgtg atgtcaaaga ggtgactggc ccgaactggc
    31021 gcggcggtgt ggcgactatt ggcgtaacgc gagagggtaa accggaaacg gtgctgtatg
    31081 cggaaaaacg ttattacaac actgccgggt cgatgatgac cgaagcggca attgacggcg
    31141 gcatcacgcg tgacctgtac gccgcgctcg gtgaagagct ggaaaacggc gcgtgggccg
    31201 tgcgtcttta ctacaaacca tttgttcgct ggatttgggc gggcgggctg atgatggcgt
    31261 tgggcggact gctgtgtctg tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa
    31321 ctgcgccgga ggccgtatga agcgcaaagt attgttaatt ccgttgatta tcttcctggc
    31381 gattgccgcg gcgctgctgt ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa
    31441 tctggaatcg gcgctcattg gcaagcctgt gccgaagttt cgtctcgaat cactggacaa
    31501 tccggggcag ttttatcagg cggatgtgct gactcagggc aaaccagtac tgcttaacgt
    31561 ctgggcgact tggtgtccga cctgccgtgc ggaacatcaa tatctgaatc agctttcagc
    31621 gcagggcatt cgcgtggtcg gcatgaacta taaagacgat cgccagaagg caatcagctg
    31681 gctgaaagag ctgggcaatc cttacgcgct aagcctgttt gatggcgacg gcatgttagg
    31741 gctggatctc ggtgtctatg gcgcgccaga aaccttcctt attgacggca acggtatcat
    31801 tcgctatcgc catgcgggcg atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct
    31861 gtgggagaaa tacagtaagg aggccgcaca atgaggtttt tattgggcgt gctgatgctg
    31921 atgatctccg gctcagcgct ggcgaccatc gacgtgttgc agtttaaaga tgaagcacag
    31981 gaacaacagt tccgtcagct cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc
    32041 attgccgatt ccaactcgat gattgccacc gacctgcgtc agaaagtgta tgaactgatg
    32101 caggaaggta aaagtaaaaa agagattgtc gattatatgg tggcgcgtta cggcaacttc
    32161 gtcacttacg atccgccgtt aacgccgctg accgtgctgc tgtgggtgct gccagtagtg
    32221 gctattggca ttggcggttg ggtcatatac gcccgctcgc ggcgtcgggt acgcgtggtg
    32281 ccggaagcgt ttcctgaaca aagcgtgccg gaaggtaagc gtgccggata tgttgtttat
    32341 ctgccgggta ttgtggtggc gttaattgtg gctggcgtca gctactacca gactggcaat
    32401 tatcagcagg tgaaaatctg gcagcaggcc acggcacagg ctccggcgtt gctggacagg
    32461 gcgctggacc cgaaagccga tccgctcaac gaagaagaga tgtcgcgcct tgcgctgggg
    32521 atgcgtactc aactgcaaaa aaatccggga gatatagaag gctggattat gttgggccgc
    32581 gttggcatgg cgctgggtaa cgccagtatc gccaccgatg catacgctac tgcgtatcgc
    32641 ctcgatccga agaacagcga tgccgcactg ggatacgctg aagcgttgac tcgttcatct
    32701 gatcccaacg acaaccgcct cggcggtgaa ctgctacgtc agctggtgag aacggaccat
    32761 agcaatatcc gtgtgctaag catgtatgcg tttaatgcct ttgagcagca gcgatttggc
    32821 gaagccgttg ccgcgtggga gatgatgttg aaactcttac ctgccaacga tactcgccgt
    32881 gcggtgattg aacgtagtat cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa
    32941 taaaaagagg cccgatggtg atgccatcgg gctagactga tgacaaagtg cgcttagttt
    33001 atgccggatg cggcgtgaac gccttatccg gcctacaaaa ttgtgtaaat tcaataaatt
    33061 gatcgatcca cgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgtcataaa
    33121 tctcagcccg atgataaatc catcgggcta tttttattgt gccccgcgtt gttgcaggaa
    33181 cagaatggtg gccgccacgc gtgagcggac attgagttta cgcagcagat tgcgaatatg
    33241 tacttttact gtctgctcgg aaatattcaa caccgatgca atctgtttat ttgacagccc
    33301 ctgtgccagc tcgtgcagaa catccagctc gcgctccgtc agcacgctga agggatcttc
    33361 ttccgcgcca aacatttcac gttcacgtaa gtactgattg acgcgttcgc taaagacttt
    33421 gctgcctttc gctccggtac gaatcgcttc cagcaatact tccgggtcgc tgtctttcaa
    33481 cagataaccg tctgcgcctg cgtctatcag cgcaaagaca tcgctggagg catcggatac
    33541 ggtcaggata ataatttgcg cggtaacgcc atccctgcgc aaggcattga gggtatccag
    33601 gccactcata cctttcatat tgagatccag caagatcacg tcgatatcca gtctattcgc
    33661 cagatcgatc gcgctcgcgc cgtcgcccgc ttcggcgacc acttcaaagc caggatcaag
    33721 ctccagtaac tgacgaacac cgcgtcgcat aagtggatga tcatccacaa tcatcacctg
    33781 aaaaggcgtt gcttcaggca tagtagtctc ctgaggtttt attagaatca ttatgtttga
    33841 gcgtagcgtt ggtaacgcta ccaggcgcgt gaatgttacc ccattttatg tatggacatg
    33901 tagtgaaaac tacaaccttg tcgttcgctt tggcatcatt ttcattataa agacggaatt
    33961 ttgcatcagc ttctcatcaa gaaaaacaac taaaaaatca atcacaatac ttgtgcattt
    34021 ctttaaacag tttttaaaaa ctgactccgg gtatggagct atgggtattt tctgtaccca
    34081 atgcttttaa ctgcaattaa tttcatagga tgaaagctca atgcatcaat ctggttctgt
    34141 ttctctttgt cgttccgcaa tatctgttct ggtggctaca gcgttatatt cacccatagc
    34201 attggcatca acagttgagt atggtgagac agttgatggt gttatcctgg aaaaagatat
    34261 ccagctggtt tatgggaccg ccaataatac gaaaatcaat cctggcggag aacagcatat
    34321 aaaagaattt ggtgtaagta gtaatactga aattaacgga gggtatcaat acattgaaat
    34381 gaatggtacc gcagaatact cagtattaaa tgatggttat caaattgttc aaatgggtgg
    34441 cgcggcaaac cagactaccc tcaataatgg tgtgctacag gtttatggcc cagcgaatga
    34501 taccacgatt aaaggcgggc gcttaatcgt tgaaaaagat ggggggaccg tttttgccgc
    34561 tatcgaaaag ggaggactac tggaggttaa agagggggga tttgcgcttg cggtagatca
    34621 gaaagcaggc ggtgctatta aaacaaccac gcgggccatg gaggtattcg gaacaaaccg
    34681 tctcggtcag ttcgatatca agaatggtat tgctaataat atgttgttgg aaaacggcgg
    34741 aagtttgcga gttgaagaaa atgacttcgc ttataatact actgtggata gtggcggctt
    34801 actggaggtt atggatggcg ggactgcaac tggcgttgat aaaaaagcag gcgggaaatt
    34861 aattgtctca acgaatgcgc tggaagtgag tggtacaaac agtaaaggcc aatttagtat
    34921 aaaggatggt gtgtcaaaaa attatgaact ggatgatggt tccgggctca ttgttatgga
    34981 ggacacgcag gccattgaca ctatccttga tgagcatgcc actatgcaat cgctgggaaa
    35041 ggatactggt acgaaaatgc aggcaaatgc ggtatatgat ctcggtcgat cagaccagaa
    35101 tggaagtatc acgtattcct caaaagccat ctctgaaaat atggtaatca acaatggccg
    35161 cgctaatgtc tgggctggca caatggttaa cgtgtcagtc agagggaatg atggcattct
    35221 tgaggtcatg aagccgcaaa taaattatgc acccgctatg ttggtgggta aggtagtggt
    35281 ttctgagggc gcttctttta gaacgcatgg tgccgtggat accagcaaag cggacgtttc
    35341 gctcgaaaat agcgcatgga ccatcattgc cgatatcact acgacgaacc aaaacactcg
    35401 cctcaactta gccaaccttg cgatgtccgg cgcaaatgtg attatgatgg ctgagccagt
    35461 gactcgttca tctgtgacgg caagtgcgga aaatttcatt acgttgacca ccaataccct
    35521 gtcgggaaac ggcaattttt atatgcgtac cgatatggcc aatcatcaga gcgatcagct
    35581 caacgtcacc ggtcaggcaa caggtgattt caaaatattc gtgacggaca ccggtgccag
    35641 cccggcagca ggagatagcc ttacactggt aacaacgggc ggcggtgatg ctgcatttac
    35701 gttgggcaat gccggaggcg ttgttgatat cggtacgtat gaatatacct tgctggataa
    35761 tggcaaccat agctggagcc tcgcagagaa tcgcgcgcaa attacccctt caaccactga
    35821 tgtgctgaac atggcagccg cacaaccgct ggtatttgat gcagaactgg acaccgtgcg
    35881 tgagcgtctt ggtagcgtaa aaggcgttag ttacgatacg gtgatgtgga gttcggcaat
    35941 taacacccgc aacaacgtga ccactgatgc gggagctggt tttgagcaaa cattgacggg
    36001 cctgacgctc ggtatcgata gccgtttctc ccgtgaagaa agcagtacaa ttcgcggctt
    36061 gttctttggt tactctcatt ctgatattgg ttttgatcgc ggcggaaaag gtaatatcga
    36121 tagctatacc ctgggggctt atgccggttg ggagcatcag aacggtgcct atgttgatgg
    36181 ggtggtgaaa gttgaccgtt ttgccaacac catccatggc aagatgagta atggggcaac
    36241 agcgtttggc gattacaata gtaacggcgc gggtgctcat gttgagagcg ggttccgttg
    36301 ggttgacgga ttgtggagtg ttagacccta tctggccttt accggcttta ccacagatgg
    36361 tcaggactac acgttatcaa acggcatgcg cgcggatgtg ggaaataccc ggatattacg
    36421 cgctgaagcg ggaacggcgg taagctatca catggacctg caaaacggta cgacgctgga
    36481 accctggctg aaagccgccg tgcgtcagga atacgccgat tctaaccagg tgaaagttaa
    36541 tgacgatggc aaatttaata atgatgtggc tggaaccagt ggcgtttatc aggctgggat
    36601 aaggtcatcg tttaccccga cgttaagcgg tcatttgtca gtcagctatg gcaatggcgc
    36661 aggggtagaa tcgccgtgga atactcaggc gggtgtggtc tggacgttct gataacagaa
    36721 aataaacagg ctgtgatgtg tcacggtctg tttatcgaat taattgcaga tataaaaaaa
    36781 ccaaccgtaa gggttggttt tttcttggga tttttggtcg gcacgagagg atttgaacct
    36841 ccgacccccg acaccccatg acggtgcgct accaggctgc gctacgtgcc gactcgtggc
    36901 tgctaatact accgttttcc acaccgattg caagtaagat atttcgctaa ctgatttata
    36961 attaatcagt tagcgataaa acgcttctcg tctgtcagca cttgcaataa caaacttaac
    37021 tgtggttttt catctttcac tctttcacca cgtaagttgt aagtgcggta tttaccgtta
    37081 ttgttcagca ccagcgtctt tttcggggtg gtaattgcca gcgtatcgtt atccgcagca
    37141 gtaacccagt aatgacggcg ttgagggttg aacaaatcct gaccttgtga atattcgctg
    37201 gcaggtgtgc tgacatgcag caggcgttgc atcagcgtcg tcatcagatc ggtatgatca
    37261 gtcagagcat taatacgctg cgccggcgtg cctggccagt gaatcactaa tggcacctgc
    37321 agatgaccgt gggaccagtc aaaggtttct tcctcttcgc tcagtggaat accccgaccg
    37381 gcagtgataa tcaccaccgt attgtccagt ttgccagaat cacgcagtgc attgagcacg
    37441 cggttgatct ggtcatcgac attgcctgcc gcccggctat atttccgtgc aaatgcctgc
    37501 tgattgctgt cgtcaatgtt agtgccattg aaagaaaccc acgagaacca gcggttatct
    37561 tcttgtgcgt agcggcccag ccagttgatc cactgcgtgg cggtctgatc gtcagattgg
    37621 gtgcgcacgc tcggcatcga gaaatctgac aacaatgcct ggcgatacag cgggctggta
    37681 aagccatctg atgagaataa ccccagctga tagccttgct gattaagcgc agtaattaat
    37741 gccgcaggcg tacgggtcga cagaatgccg tccatatagc tcggcgagat gccatagaac
    37801 aggccaaaga tgccgttgtc tgtagtgttg ccggagctca tatggcgcgt gaacgaaata
    37861 ttttgctcag caaaacctgc cagcgcaggc atctgcttct cgaagcgtga gtagttcagg
    37921 ccatcgacag taatcaacag cacattctgc ccggtgccca tatcgcgata gcgcagttcg
    37981 cttaacggat actgtacgga aacggcgtct ggattacctt gctcaataag acggcgttga
    38041 tactcctgcg catcaagcag gccatgcttc tcaaggaaac gtcgcgccgt catcgggtac
    38101 gaaagcggca ggttagcgcg ctgcatggtg atcgggcgat agaagttggc atcggcccag
    38161 atatacacca catgcgaggc gataaaggcg ataaataaga atgcggccag cgggcgcgcg
    38221 aagcgtcgac gacgcgtcag gctgcgcagc ttttgccagc tccacgtcgc aaacaccagt
    38281 tcaagcaata aaataaccgg cacgctgatg aacatcagct gccagtcgcg cgccatctca
    38341 ttttcgtctg ggttgataac caactgccag acgatgggat taagatggag atggaaacga
    38401 gtaaagactt cgctatcgat cagtaatagc gtcattcccg ccgtcgccag aatgacggac
    38461 aaaaacctca tcagcctctg ggagccgacg ataaaggtca gcgggaagag gatcagcaag
    38521 taggtggcga acaccaggaa gctgaaatgg ccgataatgc ttacgtagga ataaatgcga
    38581 ccagcaagcg ttgtcggcca gtcggcgata aacaggtaac ggctgccaat gacgagcgaa
    38641 agcagaatat tgaacagtgc aaaccagtgc ccccaactga ccatctggga gactttttca
    38701 cggtagcgct gacgatgagt taccataacc tgttatctgt ttcccttagt gcgctttgtc
    38761 ttcgttgata gaggcctgta aggcgcgggc aaaagagttg gcaattgcct ggcgttgggc
    38821 cggggcaatg ctggtgttga taaggttagt gaccatgttg cctaacacca tcagggaaag
    38881 gtcggtcgga gccttatgtt tttccagtac gttgagcagc tctgcgagca attgttcaac
    38941 ctgttcatta ctgtagcggg aaatttgtgg cataaatcga aatcagttag ttgatgaaag
    39001 ggcaacatat taccgtagca acggtttttt ttctgctttt ttatgcgtta aatcattctc
    39061 gttacgctgc cttctgtaaa atgaccgcca tcttttattg cgtcgcttaa ccggcggtgg
    39121 ttgaataccg cccggtctta aaggagagtt tatcatgagt ctggatatca accagattgc
    39181 tctgcaccag cttatcaagc gtgatgagca gaaccttgag ctggtgttgc gcgattcatt
    39241 gctggaaccg acagaaaccg tcgttgagat ggtggcagaa ctgcatcggg tctatagcgc
    39301 caaaaataaa gcttacggct tgtttagcga agagagtgaa ctggcgcaga cgctgcgctt
    39361 acagcgtcag ggcgaagagg actttctggc atttagccgc gcggcaaccg gtcgcctgcg
    39421 tgacgaatta gcgaaatatc cttttgctga cggtggattc gtgctgtttt gccactaccg
    39481 ttatctggcg gtggagtatt tgctggtggc agttctgagc aacctgagca gtatgcgcgt
    39541 taacgaaaat ctcgacatca acccaaccca ttatcttgat atcaatcatg cggatattgt
    39601 tgcgcgtatc gatttaaccg aatgggaaac caatccagag tccacccgct atctcacttt
    39661 cctgaaaggg cgagtagggc gcaaagtggc ggatttcttt atggatttcc tcggggccag
    39721 cgaaggattg aacgcgaaag cgcaaaaccg tgggctgttg caagcggttg atgatttcac
    39781 tgccgaagca caactggata aagcagagcg acagaacgtg cgccagcagg tttatagcta
    39841 ctgtaatgag cagctgcagg caggcgaaga gatcgaactg gaatcgctgt cgaaagagct
    39901 ggcaggcgta agcgaagtga gcttcacgga gtttgcggct gaaaaaggct acgaactgga
    39961 agagagcttc ccggcagacc gtagcacact gcgtcagttg acgaagtttg ccggtagcgg
    40021 cggtggcttg actattaact ttgatgcgat gctgttaggt gaacgcatct tctgggatcc
    40081 agcaaccgat accttgacca ttaaaggcac gccgccgaat ttgcgcgacc aattgcagcg
    40141 ccggacgtct ggcggcaatt aatcggtttt gtgttctgaa tgccagatgc ggcgtgaacg
    40201 ccttatctgg cctgcaaaaa atagcaatac cgtaatcagg cataatctgc agatacaaaa
    40261 aaccccgccg gagcggggtt ttttacaact tattcagcaa ttaagcgcga acgaagtcga
    40321 tgtgctgcag cttcggtttg tacgggtgac gctgtacgtc ctgagcttta actttgattt
    40381 ctttaccgtc aacaacgatg gtcagaactt cgctgtagaa ttcagcttta gcttgcatgt
    40441 tcatgacttt gtcgtgatcc agctcgatag ccagcggtgc ttctttgcca ccgtagatga
    40501 ttgccgggaa cttgttagcg gcacgcaggc ggcggctcgc acccttaccc tgctctttac
    40561 gtacttctgc gttgatagta aacatttctt tctctcttaa attaatcctg ctacaggcga
    40621 cccagcaaca agtaagtgat ctgctttgcg tatgcaaaag cgggcgagat tctatactca
    40681 atcgccttat acatcaatca aaactaccat taaccgcgta attcgtgcgc ccgacgaaaa
    40741 cgaccttcgt aatcgaacac tttttcacgc acttgccagt actgaccttt catgcgggcg
    40801 acgacaaaat ccggatgtcg cagtaaggct tgctgggcga ggatatcggc ggcggtgatc
    40861 cagcgcagag ggatgcccgg tgtgcgcgta tgcgggcgga taaaaagctg ctcgaaggcg
    40921 gtacgctggg cgggcgtttg cagacggaaa cgctcactca catccgcgcc gtcttcatcg
    40981 taataggtga ttttcaacca ttcgcctttc tcgtcgtgcc catgctgcaa agacatgccg
    41041 ctacagcgta ataccagcgc atctttcagt cgtagcgccg cttttaacat atcatccgga
    41101 tcaaccagta ccgtgtcaca ttcgcggcag cggcgagcgg caatatcatt ttctgcgtta
    41161 cattgcgggc aatttttaaa acggaaacgg aagtcacact gttcgcgatg accgtcgtca
    41221 tcttcaaacc atccctgaca gcggcgacca aagtgttcaa tcaatgtccc gtcggcggtc
    41281 gttttccccc aaaaggtgtt ggcaaaaccg caggcagggc agaaaacctg taccggaacg
    41341 ttgtcacttt tgcctttcgg tgtaccaact tccggcgcgt agagatcgtg aggattaccc
    41401 gcataatcaa gaattaaaca atcagtcttg cccggagcga gacgcagacc gcgcccgaca
    41461 atttgttggt aaagactcac tgattcggta gggcgcagaa tggcgataag atcgacgtgc
    41521 ggggcgtcaa atccggtggt cagtactgcg acgttgacca gatagcgaaa acgctgggct
    41581 ttaaaatctt caattaacac atcgcgctca gcgccggggg tgtcgccagt aatcagtgca
    41641 gcatcttcgg caggcagtaa tcccacaatc tcttttgcgt gttcaacggt ggcggcgaaa
    41701 atcattaccc ctttgcgctt ttcggcgaac tccatgatct ggctgatgat atgtggcgta
    41761 atgcgttgct gttttttcag ctcacggttg agatcagctt cgctgaacag cccgttactc
    41821 tgtgcctgca agcggctgaa atcgtattgc actactggca tatccagtcg ttctggcggc
    41881 gtcagataac cgtgtttaat catataacgc agcggcagct cataaatgca gtcacggaaa
    41941 agggctttct catcgccgcg taccatgccg tgataatgga actggtagat ccagcctttg
    42001 cccaatcgaa aaggcgtggc agtcagcccc agcaggcgta agtggggatt cactttagtc
    42061 aggtgagtga ggatttgctg atactggctc tcttcatcgt caccaatacg gtggcattca
    42121 tcgacaatca acagcgaaaa ttcaccctga aaggcatcaa gattacgggc gaccgactga
    42181 acgctgccaa ataccacttt accgtggctc tctttgcgct ttagcccggc ggcaaaaata
    42241 tcggcttcca gccccagcgc ctgatacttt gcatggtttt gcgccaccag ttctttaacg
    42301 tgtgccagca ccagtacgcg accacgcgcc agccgtgcca gttccgctat caccaggctt
    42361 ttacctgcgc cggtgggcag cacgataacg gcaggggttt tatgacgacg aaaatggttg
    42421 agcgtggcat ccacggcttc ttgctgatat gggcggagtg taaaaatcat ggtttcgcgt
    42481 aggtagatta ttctgctaat agtatgccac ggttcttttt cccttgaggg aagttattag
    42541 accgctatac tgggcagaca acagttcaga ttttcgggtc attacccgac ctccgttttt
    42601 tacaggcaaa atcacacaca tgcgacttga taaatttatc gcacagcaac tcggcgttag
    42661 ccgtgctatt gccgggcgtg aaatccgcgg caatcgtgtc accgtcgatg gcgaaatcgt
    42721 tcgtaatgca gcgttcaaac tgctccctga acatgatgtc gcttacgatg gcaacccgct
    42781 ggcgcagcaa cacggtccac gttacttcat gctcaataag cctcagggct atgtttgctc
    42841 cacggacgac ccggatcacc cgacggtgct ctattttctg gatgagccgg tagcgtggaa
    42901 actgcacgcc gcaggacgtc tggatatcga taccaccggt ctggtgctga tgactgatga
    42961 tggtcagtgg tcgcaccgca ttacttctcc gcgccatcat tgcgagaaga cctatctggt
    43021 gacactggaa tcacctgtag ctgacgatac ggcagagcaa tttgctaaag gcgtgcagct
    43081 gcataacgaa aaagatctca ctaagccggc ggtgcttgaa gtgattaccc caacgcaggt
    43141 tcgtctgacc atcagcgaag ggcgttatca tcaggtgaaa cgcatgttcg ccgccgtggg
    43201 taaccacgtg gttgagctgc atcgtgaacg tattggcggt attacgctgg atgctgattt
    43261 agcccccggt gaatatcgtc cgttaactga agaagaaatt gccagcgtcg tctaactttt
    43321 caatgacttt caggagcccg ttgtgaccac ccgacagcat tcgtcgtttg ctattgtttt
    43381 tatccttggc ctgctggcca tgttgatgcc gctgtcgatt gatatgtatc tgcccgcgct
    43441 accggtaatt tcagcgcagt ttggcgtatc ggtgggcagt acgcagatga ccctcagtac
    43501 ttatattctg ggctttgcgt tggggcagtt aatctacggg ccgatggcag acagcttcgg
    43561 gcgtaagccg gtggttcttg gcgggacgct ggtgtttgcc gccgccgcgg tggcgtgtgc
    43621 gttggcacaa accatcgatc agctgattgt gatgcgtttc ttccacgggc tggctgcggc
    43681 tgcggccagc gtggttatca acgctttgat gcgtgatatt tacccgaaag aagagttctc
    43741 gcggatgatg tcgtttgtca tgctggtgac gaccattgca ctgctgatgg caccgatagt
    43801 tggcggttgg gtgctggtgt ggctgagctg gcattacatc ttctggatcc tggcattagc
    43861 ggcgattctg gcttcggcaa tgattttctt cctgattaaa gaaaccttac caccggagcg
    43921 tcgtcagcca tttcacattc gtaccactat tggtaacttt gcggcgctgt tccgccataa
    43981 acgtgtcctg agctacatgc ttgccagtgg tttcagcttt gccgggatgt tctcattctt
    44041 aagcgccgga ccgtttgttt atattgaaat taaccacgtc gcgccggaaa actttggtta
    44101 ttactttgcg ctaaacattg tttttctgtt cgtgatgacc atctttaaca gccgcttcgt
    44161 ccgccgcatt ggcgcgttaa atatgttccg ctcggggttg tggatacaat ttattatggc
    44221 agcgtggatg gtcatcagtg cgctgctggg gctgggattc tggtcgctgg tggttggcgt
    44281 tgcggcgttt gtgggctgcg tgtcgatggt gtcatccaat gcgatggcgg tcattcttga
    44341 tgagtttccc catatggcgg gaacggcatc ttcgctggca ggaaccttcc gttttggcat
    44401 aggggcaatt gttggcgcat tgctttctct tgcgaccttt aactctgcat ggccgatgat
    44461 ttggtcaatt gcattctgcg caaccagctc cattctcttc tgtctgtacg ccagtcggcc
    44521 gaaaaaacgg tgatctattg cacaacgagg aaggtaaaag gcttcctttg ttgatgcatg
    44581 tcaaccacaa atctatcatt tccccgatat atgtttattt tatgtaaaat caatttatgt
    44641 aaaaagtcac atcattgtag ttaaaaaggt tgagttagat cgcagaaacg ggtacatata
    44701 gccccgcaaa cgtgaccacg cccgcagata ttacttaaat cagagccata gaggccacgc
    44761 aggcgaggca tcaatcttta cgatctgtat aaagacggat tgttgatgat gtgttaaaat
    44821 tgatgtaaac aaattgtgaa gtgaatgtac ttccggggaa aataagtgac ttcattacaa
    44881 ctctcaatcg tccatcgact gccgcagaac tatcgctggt ctgctggttt cgcaggttcg
    44941 aaggttgaac cgattccgca aaatggaccg tgcggtgaca acagcctggt ggcgcttaaa
    45001 ttgcttagcc cggatggtga taatgcatgg tcggtgatgt ataaactaag ccaggcgtta
    45061 agcgacatcg aagttccatg ttcggtgctg gagtgtgaag gtgagccgtg cctgtttgta
    45121 aatcgccagg acgagtttgc tgcaacatgc cgattgaaaa attttggtgt ggcaattgcc
    45181 gaaccctttt caaactacaa tcctttttaa gcgtcagctc aacgccagta actgacgcgt
    45241 atactcctgc tgcggtgtgg caaacacgcg cgcgcacggt ccttgttcca ctacttcccc
    45301 ttgtcgcagt acgataacct gatgacataa cgcgcggaca acgtgcaaat cgtggctgat
    45361 aaacaaatag gccagttgat gcttttgttg caatgatttc aaaagcgtca atatttgcgc
    45421 ctgtaccgtt ttgtcgagtg atgatgtcgg ttcatcaagt atgatcagcg agggcttaag
    45481 aattaatgcc ctggcaatcg taatacgttg tcgctgacca ccagagaact ccgccggata
    45541 acggtggcgt gtttcaggat ctaatcccac ttcatgcatc acggctatca cttgttgttc
    45601 gcgttgtgcg gcagaaagcg tcggctggtg aaccagtaag ccttcctcaa taatctgcaa
    45661 aacgttgagt cgtgggttga gcgaggagtt tggatcctga aataccacct gaatacgatg
    45721 acgagtaggt aacagctggc ggcgatttaa atcttgcagt ggctgaccgt caaagacgat
    45781 gctgccctga gaggtaatca gtcgcagcag cgccagtccc gtcgtgcttt tcccggaacc
    45841 agactcgccc actaaaccca gtgtttcacc cgcccgtagc gtaaaactga tgtttttcac
    45901 caccacatta tgatccacaa tgcgcttcaa aatccctttg cgaatgggga aggcaacctg
    45961 aagctgttca acatccagca acgttgaggc aggttctggc aacggtaccg gatcgcctga
    46021 cggttcactg ttgagtagct tttgtgtgta aggatgagtg ggtgaggcaa atagcgtagc
    46081 ggcgttattt tgctcgacac agcgaccgtt ttgcattacc gccacgcggt gggccagttt
    46141 tctgacaatg ctgaggttat gagtaataaa cagcatgccc atattcagct cgccttgcag
    46201 ttcgcgcaac agctgtaaaa tctgcgcttg gacagagacg tccagtgcgg tggtcggttc
    46261 atcggcaatt aataattccg gtcgcgttaa cagcgccatc gcaatcatca cccgctggcg
    46321 ttcgccgccg gagagctgat gcggataatc tgtcagccgt tttgccgcct ggcggatacc
    46381 gacgcgatca aggcagttaa gaatttcgcc acgagccgct tcccgacgca tcccgcggtg
    46441 gagtgaaagc acttcataaa gctgtttttc cagggtatgc aatggattta acgacaccat
    46501 cggttcctga aaaatcatgg cgatcttatt accgcgtaca ccgcgcaacg tttgatcgct
    46561 ggcatgaagc agcgattcgc catgaaaacg aatatcgccg gagagatatt caaccggcgg
    46621 ggaagggagc aggcgtaaaa ttgacagcgc ggtaacgctt ttgcctgaac ctgactcacc
    46681 caccagcgcc agcgtttcgc cagcctcaat ctgtagtgaa acatcattga ctactgtacg
    46741 tacggtttgc tgatggcgaa aacccaccga caaattttca atcgctaaca gagtttgcgt
    46801 catgctacac cgccttatta ggatcaaatg cgtcgcggac ggcttcacca ataaagatca
    46861 gcaaagacaa taatatcgcc accgacaaga aggcggtgat cccaagccac ggggcctgaa
    46921 ggttattttt cccttgtaac agcagttcac cgagtgacgg tgaaccgagc ggtagaccga
    46981 agccgaggaa atcgagcgag gtcagggtgg ttatcgaact acataaaata aacggtaaaa
    47041 aggtgagggt cgcgaccatg gcattaggca acatatgacg caggatgata ctgcgatcgc
    47101 tgacgccaag cgcctgtgcc gcacgaatgt agtcgaaatt acgagtacgt aaaaactccg
    47161 cccgcaccac gccgaccaga ctcatccagc caaacaagat agtaattgcc agcagccacc
    47221 agaagttagg ctgtacgacg ctggaaagta aaataatcaa aaacagcgtc ggcatccccg
    47281 accatacttc aataaagcgt tgcccccaga gatcgacttt accgccgtaa tagccttgta
    47341 gcgcccccgc cagcacgccc atcacgctgg aacagagagt cagcatcagg ccaaacagaa
    47401 ccgagatccg cgtgccatag agaatgcgtg ccagcacatc gccgccgttt gcatccgttc
    47461 ccagccagtt ttgccgggag ggtggagaag ggaagggctt atcggtagca aagttgatac
    47521 tggtagcacc aaagcgaatc ggtgcccaca gtacccagcc gttattttcc agccgttgtt
    47581 tcagccacgg gtcctgataa tcagcctgac ttgccagcgg gccgccaaaa tcgctttcgc
    47641 tgtagttttt caacaacggg aaataccaac tgccgtcata acgcaccagc aacggtttat
    47701 cgttagcgat aagttcagaa cacaaactca aaccaaacaa aacgaggaaa atccataacg
    47761 accagtagcc gcgacggtta tgacgaaaac gcgcccaacg ggcctgattg acggggctga
    47821 gtcgcgacat taacgtccct caaaatctat acgcggatca accagcgtat agctgatatc
    47881 actgacaata ttcagcagca ggccaatcag ggtgaaaata tacaaggtac caaacattac
    47941 aggataatcg cgcgagacgg tcgcttcgta gcccagtaag ccaagaccat tgagtgaaaa
    48001 catcacctca atcagcagcg agccggtaaa aaacatgctg ataaacgtcg ccggaaaacc
    48061 ggcaatcacc agcagcatgg cgttgcggaa catatgtttc cagagaatgt ttttctcact
    48121 tacccctttc gcacgcgcgg tcaccacgta ttgcttgcgc acttcatcaa ggaatgagtt
    48181 tttggtcagc atggtcagcg ccgcaaagcc accaatcact gtcgccagca ccggtagcgt
    48241 gatatgccac agataatcgg tgattttctg ataccacggc agcgaatcaa agttagcgga
    48301 aaccaggccg cgtagaggga acaggtcgaa atagctgccg cccgcgaaga agacaatcag
    48361 caggatggca aacaaaaagg ccggaatggc gtagccgatg atgataaatg cgctactcca
    48421 gacgtcaaag cgactcccat tataaacagc tttgcgaatg cctaacggaa tcgacaccag
    48481 atagataatc agcgtgctcc acaatccgag ggtgatggaa accggcagac tgtctttaat
    48541 cagcgtcagc accgaggcgc tgcgaaacag gctatcacca aaatcaaagc ggatgtagtc
    48601 ccagagcatt ttgaagtaac gttcgtggat cggcttatcg aaaccgtagc gatgagtgat
    48661 ctcagcgatc acttctggat ctaatccgcg tccgccacgg taattactgt cgctgatatt
    48721 gccgacaccc gtttgcgcat ggctggcacg aacaccttca ccgcctgcgc cgggtaatac
    48781 tccggcatta ccaaactcaa tggcggcgat ggcctgatcg accggaccgc caggcgcaat
    48841 ttgcacgatg aaaaagttga tagtgataat agcccataac gttgggatca ccagcaacag
    48901 acggcgaatc aggtaagcgc ccatctactc tccctgttgt ctggctgacg gcagtttggc
    48961 cgctttatta acgtcatacc accaggtatc gatacccagg ctgtaaacag ggcgtacagc
    49021 gggttgggag aatttatccc accaggcgag acagtcttcc gccatgtacc acattggcag
    49081 catgtaataa ttccacgtta atacgcgatc cagtgctcgc cccaacggca gtaatttttc
    49141 tttatttccc tgcgcggcaa taatttggtt gatcagcgag tcgataaccg ggctttgcac
    49201 gccgggggca ttataagtgg aattgatata ttccgatgac caggaaatct gtaaatcgga
    49261 actgggccac ggcatcgccc gccataggcg cggcatcatg tcatagtcgc gactgcgcat
    49321 gcggttagtg atttgcgagt tatccacctt gcgaatgtcc atattgatac ccagccgttg
    49381 caggctgtgc tggaacggca atacccactg actattgctg cttgcgggaa gcaataattc
    49441 aaagctgagc ggctgacccg ttgtggcatt aacgcgttgc tgccccttca gcacccagcc
    49501 cgcttcgttg agaagtttgt cggcttttaa caggttgtca cgatcgtagc catcgccttt
    49561 agataccggc ggctggtaga tttgtgtgaa gacttctggc ggtagatctt ttttcattgg
    49621 tgccagaagc accagctccg cggcgtcggg gtaatttctg gccgcgtatt cggtattttg
    49681 aaaataactg ttcgtgcgac tccaggcgtt gtaaaacaac gccttgttca tccattcaaa
    49741 gtcaaaggcg agagtgattg cttcccgcac ccggcgatcg ctgaataccg cacgttggat
    49801 attaaacgcc agccagcgcg tatcctgggc tgattcgttc ttttgctcat ctttgatgat
    49861 gtatttttta tcgaaatttt taccggtata acgcgtggcc cagtttttag cgtcgttttc
    49921 catacgcaaa tcaaaggcac ctgctttaaa cgcttcaaag gcgacattat catcgaggta
    49981 ataatcgtag cgaatggtgt cgaaattcca gcgtccacgg tttaccggta agtttgctgc
    50041 ccagtaatct ttcacgcggg aatagacaat attttgcccc attttccagg acgtaacgtg
    50101 gtacggacca ctggcaagcg gaggcgtggc gagcgggtcg ctaagtttgt gatccttcca
    50161 gtacttttct ggaaataccg gcagcgaaaa cagactcaac atatcttctt tgccgggttt
    50221 agctaactca atgcgcacgg ttaacggcgc aatggctttg acggtggtgc ctttgtagac
    50281 cagacgaaat tgcggcacgc cttcggtcag aaatttttga aaagtaaact ctacatcgcg
    50341 ggcagtaatg ggagaaccat catgaaaacg ggcgcgcgga ttaatagcga cctccaccca
    50401 ggaataatcg tcagcatagc gtgcactttc agcaatcagc gggtaataac tgcctggttc
    50461 gtcatcggaa gtcgtaaata acgtgtcgta cagctgctcg gtgcgtgcgc ccgggttgcc
    50521 gcgcagtgca tagcggttga aattatcgaa ggtaccgagg gctgacaacg ttatctgccc
    50581 accttttggc gcggcggggt tcacataatc aaaatggttg aaattaaacg cgtaccgggg
    50641 ttcgcccagc acggcaaagg catagctttc cttgatagcc tgcgcctgcg caccgaaggt
    50701 gaacagagcg ataaacagca gcagtatgcg cacaatcatc tgcaacggat ttccttttac
    50761 tcatcattag ccgcaatccg gcatgctttc actatgaata ataagggacg taagcacacc
    50821 ttaccactgc ggcgtatacg gtttcttcag ccagcgaaca aaatcgtcca gcggtaacgg
    50881 gcgactaatc cagtagcctt gcatgaaatt aacgccgcgt tcgcttagcc atcgcgcttg
    50941 ttccggcgtt tcgacccctt cagtaactgt cagcatattg agacgtttcg ccagcgtcag
    51001 caccgcgtca agtacgggtg aagtgatcgt ttccgtaccg atggcgttga taaatccacg
    51061 gtcaattttc agataatcga gcgtaaaacg ctcaagatag ataagcgcgc tgtgcccggt
    51121 gccgaagtca tcaatagcaa tttctacgcc gaccgagtgc agccaggcga agagttgtgt
    51181 ggcttcttgc tctttcagca tatcgcgctc ggtaatttcc agcacaatct ggaaatggtg
    51241 tgcgggcagg gaagtgagca gtttctggat atctgcttta aagctttcgc tgtgcagatg
    51301 gtccggcgca atgttaatgc caaatttgac gcctaccggc agtacttttt ctaattctgc
    51361 ggcatcgcgg gcaattaact caaacaggtg ctgagtcagc ggcacaatca tcttttgcga
    51421 ttcggcaaag ttaatgaagg catccggggg aatttctccc gctacaggat gccgccagcg
    51481 tagcagtact tccaggcccg ttacccgcaa agcttgtgta tccaccaccg gttgatacgc
    51541 cacgtaaaat tgttcgcgct tgatggcggt catgatttct ctgccggggc gcatacgtac
    51601 gctcatcagg taatagcaaa gcaggccaac gacagttcca ctcatgccgc ccagtaaaaa
    51661 tgcgtaccac acatcgttcc atgtccagtc atccgcataa agccgaacgg tcagaggaat
    51721 gcccgcaatt ttagtttcac ggaccggcat gtcggttaat tcattaacgt tcatcaaacg
    51781 tgaagaaaag gtagatagcg cagtattgcc aataatgagg gcgacgccat cgtaatcttc
    51841 ctgccgtgaa ctataaaaga gagaaggcgt caggttgaga ttcagagcgg caaacacacc
    51901 gctatttttc agcaaagggt tgcgatacca gatgacgatt gcgggtttgt tcggcaccat
    51961 cggcgtgccg ggtaagatcg ccatatcgac gtttttatta atgtcgagcg ccggaatcaa
    52021 ttcattgagt ggaatgtcca tctcaccagt cgcagatgaa cagaatgttt ttttatcttt
    52081 caccagcaca aacgtacgga cattcatgct aaactccgcg cgagcggtca gttcggggtt
    52141 agcctgctgg caggtatcta aggtcagcgg ctgaagccgg tcagtagtgg atttcaggtc
    52201 ggcaaaatag gtatcgagat acttttgtac gtcgataatc agtgtgtcgt atttgacttc
    52261 gtgcttatgc caggccacta aaaactgaag gcagctaacc agtatcgcaa tcatcacgcc
    52321 tgctacaatg caggtaagca ggagcttacg tccagaattg ggagcgcgta tgaacatgat
    52381 tccgttaatt tcctgaaatt acgacaacat ttaaaagata acaatgaccg gactatagcc
    52441 tggatcaaga cgcggaaaca tcattttctc tgatgaatga aaacaaaaaa gcactgccta
    52501 agcagtgctt tcagttaact cgtcaggata gccaagggat tgcatccaaa cggtttatta
    52561 gctgcggctg agaacccggc gtgcttcgtt gtaacgcttc ttccagtacg gttcattcat
    52621 gctggaaata ataacaccac tgctggtgga agcatggaca aactgattgt tgccgatata
    52681 aataccgaca tggcgtcccg ttgaaccggc acggaacaga actaaatcac ccgtacgcaa
    52741 attactgcgg gagacagatt tacccatttc ctgctgttcg taagtcgaac gcggaagttc
    52801 taagccaaat tgctcacgga atgtacgctg tacgaaacca gaacaatcga tacctttttt
    52861 agtgctgccg cccagacgat aacgtacgcc tttccagtca gcatactgat ccataattcg
    52921 cgattttacg tcgacattac gaaccaggtt ttcaaattca tcctgagaag cttgcagtga
    52981 tgatgtttca ctacccactg cacgtgtctc aggatgcata ttctttgcgg tgttatttgc
    53041 actacatgca gaaagcagaa ccgctactgc aatcgcggga atcccgcgca agatatatct
    53101 caaaatcggt tgagatttga ccatgttgtt tgttttccct tgaagtcctt aacgacaaat
    53161 atcgttataa aaaaatgcca agcgacacga ggctaattac ctgggcatgt tgaaacaatt
    53221 ccttaaggcc aaatttgtgg actaacgcac aaaattatat gctgtgagga taatctaccg
    53281 ccgaggcaaa acgagttcga gattacccga ttggtgcggt cttggcgagc ggttttacat
    53341 gattttttat aagaaaaagt gatacagaaa gttaataagc ggggttggtc aattaaagta
    53401 caattgagtg aaacgcatgc aatccattca tttacttaga gaatgatttt tgcgttcgat
    53461 agatgaaagc acggtgcgca tcatcaggat tatcctcact ataaaaataa ccctgatgat
    53521 gttaattact gtgagttatt tgttttggaa atgtttgttt tttcctggta gtgatttgtc
    53581 aaaaaaggtg attaatcgat cacttaatgg cgtcagcaac acccagggca aaccgatcaa
    53641 tatcacggtc atcgaaccga caatgatgtc agtaaaccag tgtgcgccaa tcattactct
    53701 gggaaatgca aaaaccacaa aaatagtaag ggcgataagg cctgcaactt tgccgaaata
    53761 acgccacatg aatgccgaaa aaataagcag catcatgccg tgatcgccgg gaaagctatc
    53821 tcgtgaggca tctttcgtgg gaacagagag cagttcgctg acgcggttaa tatcggtaaa
    53881 agtcaatgtt gggctggccc gttttacagg aattaatgcc tgacccagct ggtttaatac
    53941 cactgcagtt aatagcatga ccagacccat aatcacgata cgtcgtctgc caggggcgtt
    54001 ttctttcagc cagaaactca gcatcaacat acccatcgcc agcagtgaac aaccgtcgaa
    54061 ggcgcgattg ttggtcaatg caaccagcca taaaaaggcc ttactttcga ccagtttctg
    54121 attaaagaaa taaaaaatat ccgcatcaat cggcaaccag aatccatgat taacgggaat
    54181 ataccaggaa agaaacagcg cggggccgac aatattcaac aacactattt gcggcaaatt
    54241 tttaatcata ttttctctaa ttatcttact gaaaaacgca ggcaacctta cgcggtagtc
    54301 gctaaacgaa gcttcaacag cgcgctctgc aaggcattcc agtcagcttc gctgctggaa
    54361 atcagctcaa tacggctgtc cggtggcgca acgttttgcg tttcaatgtg caggtcatcg
    54421 ccctgacggt tgattcgtac cagcccttcg ggaatacgca gcacgccttt gacgcgttcc
    54481 accggtgcaa gtcgcgccca ttccagaatg ccaatggtgt cgaataccgt atcagcgtcg
    54541 aatatccagc cgcaggcctg atatccttgc ccgctgttca gactgcgacg ccagcgttga
    54601 tgctctggca ggcttaacgc tgctaaccct tttttcacga catgctgatg agaatgcgcg
    54661 gcgctggcgg gcaactcggc taaattgcga cgcggcaaat ccagaagatg accgtcaact
    54721 ttcccatgct cactgtgaat taattgtcga tcgccaccat tttgctgcca ccaacgctgt
    54781 agcgcttgct cactttcggg cgtcgcacgg tcggatttat tggcgacaat gatgtctgcg
    54841 gcagccagct ggtcacggaa gttttcattg ctggcgcttt tttcgtccag cagcaggcgt
    54901 ggatcgagaa tgcacaaggt ggcgcgcaga tctatccacg gttcatagac tggtgcggtt
    54961 aacagatcga ggatctgttt cggatggccc agcccggtcg gctctatcaa caagcggtct
    55021 ggttttccct gacgcagtaa ggtattcaac cctacctgca tgggtaaacc attaacgcag
    55081 cacatgcagc cgccggggat ctctttcagc aatgcgccgc tatcggcgag caaagcacca
    55141 tcaattccga cttccccaaa ttcattaacc agtaccgccc atttttcgtt gggatcttta
    55201 tgggctaaca gatgaagaat cgacgtggtt ttcccgctgc cgagaaaacc ggtgatgagg
    55261 ttggtcctgg tcacaacggc tccgcaatac tctggtaagt taactgagcg ttaatcttgg
    55321 ctaaaaaagg cagaaaagag aaggggagag cggacgaagg tgagcttcat ccgctttaac
    55381 cgcagaaatc gttaaatttt gagggtgttt aatagcgcct gatgtgcgcc gaattgtacg
    55441 atttgttgcc aggcttgttc gatggcctgc acaaaatgcg ggttatctgg cagatcatca
    55501 ccgaaaactt cacgcaggga aagcagggcg gttacgcgtt gttcactgct gctgcccgca
    55561 acaagttcgc gaattttatc gctaagcgga tcgcgaacat caatggcatt tccggcatca
    55621 tcaacgccgc tgatgtaacg catccagcca gcaacgccta atgccagcaa cgaccagtcc
    55681 gtttcacgcc ccagatgtat gcgaatacct gccagcatac gttgcggtaa tttctggctg
    55741 ccatccatcg cgatttgcca ggtcttatgt ttcagcgacg gattagcaaa acgtgcaatt
    55801 aacttatccg catattgtgt taaatcgaca tctttaattt gcagtgtcgg cgcttgctca
    55861 tccagcatta atgttctggc agcatagcga aatacgcgat cctgcatgca atcactgata
    55921 tgggcgaatc ctgagaggta acccagataa gcgagaaaag agtggctgcc attaagcata
    55981 cgcagtttca tctcttccca tggcaggaca tcattcacca tttgtacacc tgcgacttcc
    56041 caggcaggac gcccagcgat gaagttatct tcaaccaccc actggataaa cggttcgcag
    56101 ctaatcgcgc agggatcatt tacccccaga tgctggctga tttctgccag tgattcgtcg
    56161 gttgcagccg gaacaatgcg gtcgaccatg gttcccggaa aactgacgtg ttcttttatc
    56221 cacccggcga gttctggcga acgtttttct gccattctca gcaccgcgtt tttcaccaca
    56281 tggccattgt cgggaatatt gtcgcaggag agcacggtaa acggtgtaag gccgcgctca
    56341 cggcggcgtt tcagtgcttc gacgagaata cccggtgcgg agtgaggttc ttcaggggtt
    56401 tgtagatcgt gaataatccg cggattactg gtgtcgagtg caccggtggc cgggtcaata
    56461 caatagcctt tttcggtaat cgtcagggaa acaattgcca cctgtggctc gcaaaatttc
    56521 tcaataattg ccgctaagga atccagtttt gcattaaggc attcgtgaac ggcaccgaca
    56581 attatcacct gattgccgtc cgcacctttc tccagcacgg tatataaatg gttctgtgcg
    56641 cggagctggc tcatcagttg atcaccgctg aacaagctga tttcacagat cccccagtcg
    56701 ccgccctggg cattcagcac acgatcggtc agtaacgcct gatgagcgcg gtgaaaggcg
    56761 ccaaagccaa aatgaacgat acgtgattgc aactgctggc gatcgtagcg tggagcgtgt
    56821 acatgatgcg ggagcgtaac ggaggcaatt gtcttcatag gagtcacgtc ttagtaacga
    56881 taaattaaca atgacagtgt aaagttatat gacagcaatt gaaattggtc tggattgtta
    56941 atgcgatgaa gtgtgagggc gatcaatcaa cgagtgaaaa ttgagggcgt aaggtgttca
    57001 ggaagcgcaa aatagaagat tggtataata aattaaaaaa gggacagcct gagctatccc
    57061 ttttctgcat gcggctgaag ttagtcagca cgccccatat aacggcgttc ttcgatatgg
    57121 atacgaattt tttcgcccgg gcttaagtat tctggtacct gaatcaccag accagtgctc
    57181 aatgtcgccg gtttgttacg ggcgctggca gatgcccctt tgatgcctgg tgcggtttca
    57241 acgatttcca gatcaacggt ctgcggcagc tcaagcgcca gcagttgacc gtcccaggtc
    57301 agcacctgca tgtccggcat gccgccctct ggcataaaca gcaactcttc ttcaatctga
    57361 tctttggtga aggtatacgg ggtatagtct tctttatcca taaagacata ttcgttgcca
    57421 tcgacatagg agaagtcaac gtaacggcgg gtcagcgtca cggtgtcaac gatatcatca
    57481 cctttgaaac gctcttctac tttcagcccg gtacggacat cagaaaaacg cattttgtac
    57541 agcgtagcgg caccgcgggc agtgggcgac tgaatatcaa tatccttagc cagcagcagt
    57601 ttgccgttgt aattcagtac catacctttt ttaatttcgt tcgctcttgg catcgaaaaa
    57661 ttcctgtagt cgaaaatgtc aaaaatatcg cgacaaagta ctcgcgacgg gcctttcggg
    57721 caagtggtat tcgcactttt gctggtgcaa aaaaggtggt actgtgcgcg ctcatcaatc
    57781 cggtggttaa ccttaagaga acaacgtatg gaatgccgtc cgggttgcgg ggcgtgttgc
    57841 actgcccctt caatttccag ccctattccc ggtatgccag atggcaagcc cgccaatacg
    57901 ccctgcattc agcttgatga acagcaacgc tgtaaaattt tcacctcgcc actgcgaccg
    57961 aaagtctgtg caggcttaca agccagcgct gaaatgtgcg gtaactcgcg tcagcaggcc
    58021 atgacctggc ttatcgatct ggaaatgctg accgcgcctt aaacatcttt aatccgcagt
    58081 aagcaaaaca gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc
    58141 cagatctcgg cgacgatgcc cgccacagat cctgcgatga tccagcccac gcgcgacgtg
    58201 ttggtataga gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc
    58261 atcccgatgc cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga
    58321 atggcaggtg aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca
    58381 acgcgcatta agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc
    58441 gggatttcca gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc
    58501 agttcgttga taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc
    58561 aatgtacaaa tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc
    58621 gtgccggtcg ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg
    58681 ccgcaaacaa taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc
    58741 gctaacgcat aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa
    58801 aaagagctga acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca
    58861 agggcaaaca tttgcgggtt agcggtcgag ccaaagctgc taagaaagac gccaacgaat
    58921 agcaaaacaa agtagttgcg attccaggca aaaagggtgc aggccagcac gcctaacagg
    58981 cagcaaaaga caatcagcga tttgcgatcg ccgcgcttat cagagcgccc agcgagaaac
    59041 tgacttacca gaatcccaat gacagcgctg ccggtaaaga agaatcccac catcgccgga
    59101 cgggcatgta cttcatcggt aaggaaaata ctgagtgtcg gggtttgcag agcacccgca
    59161 ataccggtga gaaaggcaac gattaaaaac gccgtcgagg tcaggtcaaa cgatttcgcg
    59221 ctggagactg cgggggagtt atgcatgctg gtcgatcact ggaagaggaa gacgcggagt
    59281 ttacgctgct tgtcagaaaa taaacagaca gcgaaatcaa aagcgccacg aataatcaaa
    59341 atgccatttc actgttgctg attggctgct gacgctgctg aaatgaaatt gctgataacg
    59401 gattttccca tcagcaatta ggaaaaatga caaaaaattg tgcagcacat caaacttttg
    59461 ctcataactt tacggctttc cttgcgtgct gaaacagaag tattatgctt tcttgaaacg
    59521 tttcagcgcg atcttgtctt taaccctaag ccaggttggc gctttttttc tcatagaggc
    59581 tgaatcgttt caattcagca agagaggaga actatgttcc agttatccgt acaggacatc
    59641 catccgggcg aaaaggccgg agacaaagaa gaggcgattc gccaggtcgc tgcggcgctg
    59701 gtgcaggccg gtaatgtagc agaaggctac gtcaatggca tgctggcgcg cgaacagcaa
    59761 acctcaacgt tcctcggcaa tggtattgct attccacacg gcactaccga cacccgcgat
    59821 caggtgctga aaaccggcgt tcaggtattt cagttcccgg aaggcgtcac ctggggtgac
    59881 ggtcaggtag cgtacgtggc aatcggtatt gctgccagct cggataaaca tctgggcctg
    59941 ctacgccagc tgacccacgt actgagcgat gattccgttg ctgaacaact gaagtcagca
    60001 acaacagcag aagaacttcg cgcattactg atgggcgaaa agcagagtga gcagctgaag
    60061 ctcgacaacg aaatgctgac gctggatatc gtcgccagcg atctgctgac tcttcaggcg
    60121 ctgaacgctg cgcgtctgaa agaggcgagg gcagttgacg ccactttcgt caccaaagcc
    60181 atcaatgaac aaccgctgaa tctcggacag ggtatctggc tgagcgatag cgccgaaggc
    60241 aatctgcgta gtgcgattgc ggtaagccgt gcggcaaatg cttttgatgt ggacggcgaa
    60301 acggcagcca tgctggtgag tgtggcgatg aatgacgatc agcccatcgc ggttcttaag
    60361 cgtctcgctg atttgttgct cgacaataaa gctgaccgct tgctgaaagc ggatgcggca
    60421 acgttgctgg cgctgctgac cagcgatgat gcgccgaccg acgacgtgtt aagcgcggag
    60481 tttgtggtgc gcaatgaaca cggcctgcat gctcgtccag gtaccatgct ggtcaatacc
    60541 attaaacaat ttaacagtga tattaccgtg acaaaccttg atggcaccgg caaaccggca
    60601 aacggacgta gtctgatgaa agttgtggca cttggcgtta agaaaggtca tcgcctacgc
    60661 tttaccgccc agggtgcaga tgctgaacag gcgctgaaag ccattggcga cgctatcgct
    60721 gctggtcttg gggagggcgc ataatgagca gacgtgttgc tacgatcacc cttaatccgg
    60781 cttatgacct tgttggtttc tgcccggaaa ttgaacgcgg cgaagtgaac ctggtgaaaa
    60841 ccaccggtct gcatgcggcg ggtaaaggca tcaacgtggc caaagtatta aaagacctgg
    60901 gaattgatgt caccgttggc ggcttcctgg gtaaagacaa tcaggatggt tttcagcaac
    60961 tgttcagcga gctgggcatt gccaaccgtt tccaggttgt acaggggcgc acccgaatta
    61021 acgttaagct gacggaaaaa gacggcgaag tgaccgactt caacttctcg ggttttgaag
    61081 tcacccccgc cgactgggaa cgctttgtga ctgattctct gagctggctc ggtcagttcg
    61141 atatggtctg tgtcagcgga agcttaccgt caggcgtcag cccggaagcg ttcaccgact
    61201 ggatgactcg cctgcgtagt cagtgtcctt gcattatctt tgatagtagc cgtgaagcgt
    61261 tagtagcagg tttgaaagcg gcaccgtggc tggtgaaacc taaccgccgc gagttggaaa
    61321 tctgggcagg ccgtaaactg cctgaaatga aagatgtgat tgaagctgca catgcgctac
    61381 gtgaacaagg tattgcgcat gttgttattt cactgggtgc cgaaggcgcg ctttgggtta
    61441 atgcctccgg cgaatggatc gctaaaccac cgtcagtcga tgtcgtaagc accgttggcg
    61501 caggggattc tatggttggt ggcctgattt atggcttgct gatgcgtgaa tccagtgaac
    61561 acacactgcg tctggcgaca gctgttgcag ccctggcggt aagtcaaagc aatgtgggta
    61621 ttaccgatcg tccgcagttg gccgcaatga tggcgcgcgt cgacttacaa ccttttaact
    61681 gacagcagga gaggcataat gaaaacgctg ctgattattg acgctaatct cggtcaggca
    61741 cgcgcctata tggcgaagac cctgctgggc gcggcggcgc gaaaagcaaa actggaaatc
    61801 atcgacaatc cgaacgacgc agaaatggcg attgttctcg gtgattccat cccgaatgac
    61861 agcgcgctga acggtaaaaa tgtctggctg ggtgatattt cccgggcagt tgcgcaccct
    61921 gagctgttcc tgagtgaagc caaaggccat gcgaaacctt acactgcgcc ggtcactgcg
    61981 acagcaccgg ttgccgccag cggtccgaaa cgcgtagttg cggtgactgc ttgcccgact
    62041 ggcgtagcac acacctttat ggcggctgaa gccattgaaa ccgaagcgaa aaaacgtggc
    62101 tggtgggtga aagttgaaac ccgtggttct gttggcgcgg gtaatgcaat cactcccgag
    62161 gaagtagccg cagcggatct ggtgattgtg gcggcagata tcgaagtgga tctggcgaaa
    62221 tttgctggta aaccgatgta tcgtacctct accggtctgg cgctgaagaa aaccgcgcag
    62281 gaactggata aagcggttgc tgaagcaacg ccgtatgaac cggcgggcaa agctcaaacg
    62341 gcgaccactg aaggtaagaa agagagtgca ggcgcttatc gtcacttgct aacgggcgtc
    62401 tcttacatgc tgccgatggt cgttgctggt ggtctgtgta tcgcgctttc ttttgctttt
    62461 ggtatcgaag cgtttaaaga gccgggtacg ttggcagcgg cgctgatgca gattggtggt
    62521 ggttcagcct ttgcgctgat ggtgccggta ctggcaggtt atattgcctt ctccattgcc
    62581 gatcgtccgg gcctcacgcc gggtctgatt ggcggtatgc tggcggtcag caccggttct
    62641 ggcttcattg gcggtattat tgcgggcttc ctggctggtt acattgcgaa gttaatcagt
    62701 acgcaactga aactgccgca gagtatggag gcgctgaaac cgatcctgat cattccgcta
    62761 atttccagtc tggtggtcgg tctggcgatg atctacctga tcggtaaacc agttgctggc
    62821 attctcgaag ggctgactca ctggctgcag accatgggga ctgcgaatgc ggttctgctg
    62881 ggggcgatcc tcggtggcat gatgtgtact gacatgggcg gtccggtaaa caaagcagcg
    62941 tacgcattcg gtgtgggtct gctgagtact caaacctatg gcccgatggc ggcgattatg
    63001 gcggcaggta tggtgccacc gctggcaatg ggcctggcaa caatggtggc gcgtcgcaaa
    63061 ttcgacaaag cgcagcagga aggtggcaaa gccgctctgg tattgggact gtgcttcatt
    63121 tcggaaggtg caattccgtt tgctgctcgt gatccgatgc gtgtgctgcc gtgctgtatc
    63181 gtgggtgggg cgctgactgg cgcaatctca atggcgattg gtgcgaaact gatggcaccg
    63241 cacggtggtc tgtttgttct gctgatccct ggcgctatta cgccggtatt gggttacctg
    63301 gtagcaatta ttgccggtac gctggtggcg ggtttggcct atgctttcct gaaacgtccg
    63361 gaagtggacg cagtagcgaa agcagcgtaa taaaaggtgt gttacagggc agaaatttcc
    63421 tgccctgtaa catttgatga gcgcaaagag aatgaccagc ttgtgaatgt aattttatga
    63481 atttaaatgg ttttatcttt cagtagaaac ttcaaacgtt catcaaacac taaggttatt
    63541 tcctcaaggg cagattattt tctgcccttt tttagtgttc ctgttctgtg catcccgtca
    63601 caaattcctc gcttttgtaa ttcaggcatt taatcgcatt ttgtgagcct gctcaaatct
    63661 gcccattttg cctctctggc ggtaatttac tttacgtaaa acatgcatac tatgagcaca
    63721 tgtttaaata tgaacacatg tttaaaccgt ctttgcagta tctatattct tgtggattga
    63781 cagggttggt aattttttac cagaggacgt gctatgcgcg aaaaggatta tgtcgtaatt
    63841 ataggttcgg cgaatattga tgtcgccgga tattcacatg aatcattaaa ttatgcggat
    63901 tcaaatccag gtaaaataaa atttacgcct ggtggagtag ggcgcaatat tgcacaaaac
    63961 ctggcgttgc tgggtaacaa agcctggcta ctgagcgccg taggcagtga tttttatggt
    64021 caatcgctgc taacgcaaac caatcaatct ggtgtttatg tcgataaatg cctgattgtg
    64081 ccgggagaaa atacgtcgag ttatttatca ttactcgata ataccggtga aatgctggtt
    64141 gctataaatg acatgaatat tagcaacgct attacagctg aatatctcgc acagcaccgt
    64201 gaatttattc agagggcaaa ggtcattgtc gcggactgta atatcagtga agaggcactg
    64261 gcatggattc tggataatgc cgccaacgta cccgtatttg tcgatccggt ttcagcgtgg
    64321 aaatgtgtca aagtgcgcga ccgtctaaat cagatccaca ctctgaagcc aaaccgcctt
    64381 gaagcggaaa ccctgagtgg gattgcgctg tcagggcgtg acgatgtggc aaaagttgct
    64441 gcctggttcc atcaacatgg cctgaatcga ctggtattga gcatgggcgg cgacggcgtt
    64501 tattacagcg atatcagcgg tgaaaatggc tggtctgcgc cgatcaaaac caatgttatt
    64561 aatgttaccg gagcgggcga tgccatgatg gcgggacttg cttcgtgttg ggtagacgga
    64621 atgccgtttg ccgaatctgt tcgtttcgca cagggatgtt cgtcaatggc gctctcctgt
    64681 gaatacacca ataaccccga tttatcgatt gccaacgtta tatcgttagt ggagaacgca
    64741 gaatgtctga attaaaaatt tcccctgaat tattacaaat ttccccggaa gtgcaggacg
    64801 ctttaaaaaa caaaaaaccg gttgtggcgc tggaatcgac cattatttct cacgggatgc
    64861 cgttcccaca aaatgcccag accgcaattg aagtagaaga aactattcgt aaacagggcg
    64921 cagtacctgc cactatcgcc attattggcg gcgtgatgaa agtgggttta agcaaagaaa
    64981 aaattgaatt actgggtcgt gaagggcata acgtgaccaa agttagtcgt cgcgatttac
    65041 cttttgttgt tgccgccgga aaaaatggcg caaccactgt ggcttcaacg atgattattg
    65101 cggcgcttgc cggaattaaa gtatttgcca ccgggggaat tggtggtgtg catcgcgggg
    65161 cggaacatac cttcgatatt tctgccgatt tgcaagaact ggcaaatact aatgtcaccg
    65221 ttgtttgtgc cggggcgaaa tctattctcg atttaggatt aaccactgag tatttagaaa
    65281 ccttcggtgt gccgttaatt ggctatcaga ctaaagcgct gcctgcgttt ttctgccgta
    65341 ccagctcgtt tgacgtcagc attcgtctcg acagcgccag tgaaattgcc cgtgcaatgg
    65401 cggtgaaatg gcaaagcggg ctgaacggtg gcctcgtggt agcgaacccg atcccggaac
    65461 agtttgcgat gccggaggaa tctatcaatg cagccataga tcaagccgtc gccgaagccg
    65521 aagagcaggg cgttattggt aaagaaagta caccgttcct gctggcgcgc gttgctgagc
    65581 tgaccggcgg tgacagcctg aaatccaata tccagctggt gttcaacaac gccattctgg
    65641 cgagtgaaat tgccaaagaa taccagcgtc tcgcgggtta atttctgacg ggcagaaagc
    65701 cgtcgcccgt actcatcaag cataccaaaa cctggcgctg acgccgggtt tactggcatg
    65761 aagggaaaat cattatggat ataatgagaa gtgttgtggg gatggtggtg ttactggcaa
    65821 tagcatttct gttgtcagtg aataaaaaga gcatcagttt gcgcacggtt ggagccgcac
    65881 tgctgctgca aatcgctatt ggtggcatca tgctctactt cccaccggga aaatgggcag
    65941 tagaacaggc ggcattaggc gttcataaag tgatgtctta cagtgatgcc ggtagcgcct
    66001 tcatttttgg ttcgctggtt gggccgaaaa tggatgtcct gtttgacggt gcgggtttta
    66061 tcttcgcctt tcgcgtactt ccggcgatta ttttcgttac tgcgctcatc agtctgctgt
    66121 actacattgg cgtgatgggg ctgctgattc gcatccttgg cagcattttc cagaaagccc
    66181 tcaacatcag caaaatcgaa tcttttgttg cggttaccac tattttcctc gggcaaaatg
    66241 agatcccggc gatcgttaaa ccgtttatcg atcgcatgaa tcgcaacgag ttgtttaccg
    66301 caatttgtag cgggatggcg tccattgctg gttcgatgat gattggttat gccggaatgg
    66361 gcgtaccaat tgactacctg ttagcggcat cgctgatggc gatccccggc ggtattttgt
    66421 ttgcacgtat tcttagcccg gcaaccgagc cttcgcaggt cacatttgaa aatctgttgt
    66481 tcagcgaaac gccgccaaaa agctttatcg aagcggcggc gagcggtgcg atgaccgggc
    66541 tgaaaatcgc cgctggtgta gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga
    66601 tcaacggcat tatcggcgga attggcggct ggtttggttt cgccaatgcc tctctggaaa
    66661 gtatttttgg ctatgtgctg gcaccgctgg cgtggatcat gggtgtggac tggagtgatg
    66721 ccaaccttgc tggtagcctg attgggcaga aactggcgat taacgaattc gtcgcttacc
    66781 tgagtttctc cccatacctg caaacgggcg gcacgctgga agtgaaaacc gtggcgatta
    66841 tatcctttgc gctttgtggt tttgctaact ttggttctat cggtgttgtc gttggcgcat
    66901 tttcggctat tttgccaaaa cgtgctccag aaatcgccca gcttggttta cgggcgctgg
    66961 cagcagcaac gctttccaac ctgatgagtg cgactattgc cgggttcttt attggtctgg
    67021 cgtaatgctc gatttgtaga ccggataaaa cgtattaagc gttgcatccg gcaatcgcgc
    67081 tattgcaaat aatttttact gcatcatccc ggagaattta ttctccgggt ccatctccag
    67141 cgccagtcct gacaaatgct ttctgttttt aattagatat cctttcttgc ttttaattaa
    67201 taaaccgtcg tgaatgaatt gcgcgagaac atataaaaga tgtcgataag aaacgcctaa
    67261 atactctgcc gcttgcgtat gcttttcgtg ataaagatca ccttcttgtg agagtaatat
    67321 aaatgctgcc aggcgattca ctaacggaaa tgattgattc tgagttaaag aaacaatatt
    67381 acgataattt ttatgactta aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa
    67441 taacagcgga cggtaatgtt tcatagggag cgcaaggcac caacactctt caatagcctg
    67501 caccgcacgc ggttcatggt ctttgtcgat taactcaatc tcgccaataa aacagggagc
    67561 ggcaaagaaa tcgatcagcg acacgcgacc attagcaagc gtggcgtaaa gcctggcgcg
    67621 gcctcgcgtc agataaaaca gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg
    67681 cgctaaaaaa tgaaacagcc gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa
    67741 cgcagattca ctcatcaatt gctgcttaag gtcattattg tggatttctt tcacgatgtt
    67801 tatccgctat gagatttctc atatttagca tacgtacgct gttgttaaga ttcaatcatc
    67861 ggcccggtcg aattttcttt gctgtagaaa aaaataacgc aactggaaac agaggaaata
    67921 aacaatggaa aagagaaaaa ttattctgga ttgtgaaccg ggtcatgatg atgctattgc
    67981 aatgatgatg gcagcgaaac atccggcaat agatttatta ggcatcacta ttgtagtggg
    68041 taatcagacg cttgataaaa cattaattaa tggcctgaat gtttgccaga aactggagat
    68101 taatgttccg gtttatgcgg ggatgccgca acccattatg cgtaaacaaa tcgttgccga
    68161 taatattcac ggtgaaaccg gactggatgg cccggtattc gagccgctga cccgccaggc
    68221 agaaagtacc catgcggtga aatatatcat cgataccctg atggcaagcg atggcgatat
    68281 cactctggtg ccggttggtc cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc
    68341 aatcctgccc aaaatccgtg aaattgtgct aatgggcggc gcttacggta caggcaactt
    68401 caccccatct gccgagttca atatctttgc cgacccggaa gccgcccgcg tggtgttcac
    68461 ctccggcgtt ccattagtga tgatgggcct cgatcttacc aaccagaccg tttgcacccc
    68521 ggatgtaatt gctcggatgg aaagggtagg cggccctgcc ggagagctgt tcagcgacat
    68581 catgaacttc actctcaaaa cgcagttcga atactacggc ctggctggcg gcccggtgca
    68641 tgacgccacc tgcattggtt atctgatcaa ccctgatggc attaaaaccc aggatatgta
    68701 cgtcgaagtg gacgtcaaca gcggcccttg ctacgggcgt acggtctgcg acgaactggg
    68761 cgttcttggc aagcccgcta ataccaaagt cggcatcact attgataccg actggttctg
    68821 gggcttagtc gaagagtgcg tgcgcggcta catcaaaacc cattaagtgt tgataacaag
    68881 ccgggacagt atcgcaagat gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc
    68941 aaaatgacat gaagagataa tcactatgga tgtcatgaga agtgttctgg gaatggtggt
    69001 actgctggca attgcgtttt tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt
    69061 tggcgcggcg ttagtgttac aggtcgtgat tggcgggatt atgctttggt taccgccagg
    69121 gcgttgggtc gcagaaaaag tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc
    69181 aggtagcgca tttatcttcg gttctctggt cggaccgaaa atggatacct tatttgatgg
    69241 cgcaggattt atctttggtt tcagggtatt accggcaatt atcttcgtca ctgcactggt
    69301 gagtattctc tactacatcg gtgtgatggg gatcttaatt cgcattctcg gcggtatctt
    69361 ccagaaagct ttaaatatca gcaagatcga gtcattcgtc gcggtcacca ccattttcct
    69421 cgggcaaaac gaaattccgg cgatcgtcaa accctttatc gatcgtctga atcgcaatga
    69481 attatttaca gcgatttgta gtggcatggc ctcaattgct ggttcgacaa tgattggtta
    69541 cgccgcactg ggcgtacctg tggaatattt gctggcggca tcgttaatgg cgatccctgg
    69601 cgggatcttg tttgcccgcc tgttaagccc ggctacggaa tcttcgcagg tttcttttaa
    69661 taacctctct ttcaccgaaa caccgccaaa aagcattatt gaagccgctg cgacaggggc
    69721 aatgaccggg ctgaaaatcg ccgccggtgt agcgacagtt gttatggcat ttgtcgccat
    69781 cattgcgtta attaacggta ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc
    69841 ctcgctggag tccattttag gttacctgct ggccccactt gcgtgggtaa tgggtgttga
    69901 ctggagtgat gcaaatcttg ccgggagttt gattggacag aaactggcaa taaatgaatt
    69961 tgtcgcttat ctcaatttct caccctatct gcaaacggct ggcactctgg atgctaaaac
    70021 cgtggcgatt atttccttcg cgttgtgcgg tttcgctaac tttggttcta tcggggtggt
    70081 ggtgggggcg ttttctgcgg ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt
    70141 acgcgcgctg gcggcggcga cactttctaa tctgatgagt gccaccattg ccgggttctt
    70201 tattggttta gcgtagtgtg caatttatga gccggattag atgcggtcaa gcgtcgaatc
    70261 cggcaacatt gttacaccat tggcactaat gacagcgcgt tatcggcaga cagggtaggg
    70321 ttgtttaagc tgccgctggc gcgtgaaatt gccgcgcaag ccatcgcaaa acgggcgctg
    70381 tcacggaaat tgtttccctc cagaaagcta tataccagac ccgccataaa accatcgtca
    70441 gcaccaaaac tgtctaccgt cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc
    70501 ttttcgctga aataaactga ctcatcgggc aaataaacaa acagttgctg aacaccttgc
    70561 tgatgcaatg cattcactgc ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat
    70621 aaaatttcca gctccggtaa agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg
    70681 attttgcccg ctttgaattc tgaaacggta tcgacaaaca ccgggatctc atcagcgagg
    70741 gtaaagaccc attccagcgc ctcggctgtc aggttgcaat cagccagtac cacgcccgca
    70801 tgacgaagta aatcgcgcga cccgttcagt agttgcggtg tcaactgttc cagcagatgg
    70861 gtatcgttaa tcgccagcac ggtttgatcg tctcgattgg caattgccag atacgtcgat
    70921 gtgctttgac catgcaaacg aacgcagccg gagacattca cgccggcacg gcgcgtttct
    70981 tctaggagca tttcgccata aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg
    71041 cgctaacagc gccagattgt gggcgatatt gcgtccaacg ccgcctgccg agcaatgaat
    71101 tgtaccggga tgagaagccg cttgcgggta atggatatcc gccatcccgc gaatatccat
    71161 attgattgtc cccaccacta cgcagtattc ctgctcggtg agaatgtaac ctttgccttt
    71221 aatccggcct ttgcgcatta aatccataat atgcgccgca acgcgcgaac gactgatttg
    71281 cagcatgtcc gcaatttcgt tctgctgaat cagcgggtta cgccgtaaaa ttgcaaggat
    71341 ctccttttcc cggttattca tcttcaggct accgcttttt cagtttgttg cgctttcagc
    71401 caggcgatct cttctgccca gatatccggg ttgatggttt caaggatcag cggaatgccg
    71461 tcgaaacggt cgttctgcat aatccagcgg aacgcatcat gaccgatatt gccttcaccg
    71521 aggctatgat ggcggtcaac gcggctgcca aaggtacttt tcgcatcgtt aaggtgcatc
    71581 ccgcgcagat acttaaagcc gacaatacgg gcaaaatcag cgaacgtttt ttcgcattcg
    71641 gctggagtac gcaaatcata cccggcagcg aaagcatggc aggtatcaat gcagacgccg
    71701 acgcgggatt tatcttccac gccgtcgata atcgccgcga gatgttcgaa tttaaaccct
    71761 aagttactgc cctgaccggc ggtgttttca atcaccgctg ttacaccttg agttttatcc
    71821 agcgcaatgt tgatggattc ggcaatacgc gcaaggcaat cctcttctga aatctgcatc
    71881 agatggctgc cagggtggaa gttgagcaaa gaaagcccca gttgttcgca acgctgcatt
    71941 tcatctataa aggcatcgcg cgatttttcc agagcttcag tgaccggatg tccgaggttg
    72001 atcagataac tgtcgtgggg aagaatttgc gccgatgtgt agtgatattt ttcacaggcg
    72061 gctttgaatt catcgatggt ttgcgtcgtg agcggtgcgg cacgccactg acgttggttt
    72121 ttggtgaaca aggcaaacgc ggttgcgtcg atttcggcgg cgcgaattgc ggcatttgcc
    72181 agaccgccag cagcactaac gtgcgctcca atgtatttca tgcgaggact cctgttaaac
    72241 ccgctggagg aaaacggtaa tgatagcggg ttaacaggag caagaagtag tggtttatgc
    72301 gatgacgctt tgaatcacat agttaatcgc accaccacca acaatcagcc aggcaaacag
    72361 taccagtgcc atcagcagag gtttcgcccc agcttttttc agcgcgctga cgtgagtggt
    72421 cagacccagc gccgccatcg ccattgccag caggaaggta tccagcgtta ccagcatgtt
    72481 caccacgctc tgcggtaaca ggtggaacga gttaaagatg gcaactacga tgaacaagat
    72541 ggcaaaccac ggaatagtga ttttgctttt ctcgccgctg ttcgccccag acagctgttt
    72601 aacacgcgcc gccagcagga tgaggaacgg agccagcatc atcacgcgca gcattttgga
    72661 aataactgct gcgttttctg catccgggct gatggcatga cctgccgcca ccacctgcgc
    72721 cacttcgtgc acagtagaac caatgtagat accgaaagtt tccggactaa accattgaga
    72781 catcagcgga tatatcgccg ggtagaggaa aatcgcgacg gtcccgaaga taacaacggt
    72841 tgcaacagcc acggttactt tactggcttc cgctttcact accggctcag tcgccagtac
    72901 cgcggcagca ccacagatac tgctaccggc accgatcaac caactggtgt gcttatccag
    72961 accaaacact ttctgcccca ggaagcaagc cagcaggaag gtactggaca gcgtcagcac
    73021 gtcaatgatg atcccactga taccgacatc ggcaatttgc gagaacgtca gacggaagcc
    73081 ataaagaatg atacccagac gtaataaata ttgcttggca aacagcacac caccgtcaca
    73141 gcttttccag atgtgcggat agatggtgtt gcctaaaacc atccccaaca agattgcgag
    73201 ggtgagggca ctaaacccgg cacccgcaac cgcgggaatg gaaccacccc acagggcgac
    73261 cccggtgata actgcactca gggctaaccc cggaataaaa tgccacagtg tacgatgttg
    73321 tttctgtaag gtgatattcg tcataaccct ctcctttaac cgggcataag gttacgacta
    73381 actggtttaa aaataaaatt gattatatat ttataattaa tctttataag tggtaagcga
    73441 ctatgcacat caccctccgg cagttggaag tttttgcaga agtattgaaa agtggatcaa
    73501 ccacccaggc gtcggtgatg ctggcgttgt cgcaatcagc agtgagcgca gccttgactg
    73561 acctggaagg gcagcttggc gttcaactgt ttgatcgcgt ggggaaaaga ctggttgtta
    73621 atgaacacgg gcggctgctc tatccgcgag cgttggcact gcttgaacag gcggttgaaa
    73681 tcgaacaact gtttcgcgaa gacaacggcg cgattcgtat ctatgccagt agtaccatcg
    73741 gtaactacat tctgcctgca gttatcgccc gttatcgcca tgattatccg cagttgccga
    73801 ttgaacttag cgttgggaac agccaggacg tgatgcaagc ggtgctggat ttccgcgttg
    73861 atattggctt tattgaagga ccgtgccaca gcactgaaat catttctgaa ccgtggctgg
    73921 aagacgagct ggtggttttc gccgcgccga cttcgccgtt ggctcgtggt ccggtcacct
    73981 tagaacagct ggccgctgcg ccgtggatcc tgcgtgaacg cggttccggc acgcgggaga
    74041 ttgtcgatta tctgttgctg tcacatttac cgaagtttga gatggcgatg gaattaggta
    74101 actccgaggc aatcaaacat gcggtgcgtc atgggttggg aattagttgc ctgtcgcgac
    74161 gtgtgattga agatcaattg caggcaggca cattaagtga agttgcggtc cctctgccgc
    74221 gcctgatgcg tacgttgtgg cgtatacatc atcggcaaaa acacctttcc aacgcgctac
    74281 ggcgctttct ggactattgc gatcccgcaa atgtgccgcg ttaagttgct gtacaagaac
    74341 atgctggtgc tgtgtcgatt tcgtgacgca gcgccttcag catgcattcg ccagaaaaga
    74401 gattggctgc tttacttata atccctgggc gatcatgaag gtgtcttata accgtgtatt
    74461 tctgccggaa ggattgccaa tcgtctgcta caatcgcgcc atcattttta agatggatag
    74521 catttttgta tgggttccga aactaaaact acagaagcgc cgggcttacg ccgtgaatta
    74581 aaggcgcgtc acctgacgat gattgccatt ggcggttcca tcggtacagg tctttttgtt
    74641 gcctctggcg caacgatttc tcaggcaggt ccgggcgggg cattgctctc gtatatgctg
    74701 attggcctga tggtttactt cctgatgacc agtctcggtg aattggctgc atatatgccg
    74761 gtttccggtt cgtttgccac ttacggtcag aactatgttg aagaaggctt tggcttcgcg
    74821 ctgggctgga actactggta caactgggcg gtgactattg ccgttgacct ggttgcagca
    74881 cagctggtca tgagctggtg gttcccggat acaccgggct ggatctggag tgcgttgttc
    74941 ctcggcgtta tcttcctgtt gaactacatc tctgttcgcg gctttggtga agcggaatac
    75001 tggttctcac tgatcaaagt cacgacagtt attgtcttta tcatcgttgg cgtgctgatg
    75061 attatcggta tcttcaaagg cgcgcagcct gcgggctgga gcaactggac aatcggcgaa
    75121 gcgccgtttg ctggtggttt tgcggcgatg atcggcgtag ctatgattgt cggcttctct
    75181 ttccagggaa ccgagctgat cggtattgct gcaggcgagt ccgaagatcc ggcgaaaaac
    75241 attccacgcg cggtacgtca ggtattctgg cgaatcctgt tgttctatgt gttcgcgatc
    75301 ctgattatca gcctgattat tccgtatacc gatccgagcc tgctgcgtaa cgatgttaaa
    75361 gacatcagcg tcagtccgtt caccctggtg ttccagcacg cgggtctgct ctctgcggcg
    75421 gcggtgatga acgcagttat tctgacggcg gtgctgtcag caggtaactc cggtatgtat
    75481 gcgtctactc gtatgctgta caccctggcg tgtgacggta aagcgccgcg cattttcgct
    75541 aaattgtcgc gtggtggcgt gccgcgtaat gcgctgtatg cgacgacggt gattgccggt
    75601 ttgtgcttcc tgacctccat gtttggcaac cagacggtat acctgtggct gctgaacacc
    75661 tccgggatga cgggttttat cgcctggctg gggattgcca ttagccacta tcgcttccgt
    75721 cgcggttacg tattgcaggg acacgacatt aacgatctgc cgtaccgttc aggtttcttc
    75781 ccactggggc cgatcttcgc attcattctg tgtctgatta tcactttggg ccagaactac
    75841 gaagcgttcc tgaaagatac cattgactgg ggcggcgtag cggcaacgta tattggtatc
    75901 ccgctgttcc tgattatttg gttcggctac aagctgatta aaggaactca cttcgtacgc
    75961 tacagcgaaa tgaagttccc gcagaacgat aagaaataag tttcctccct tccttgctaa
    76021 gccctctcaa ccgagagggc tttttcaatt ccatttccct gacaaatcat acggatataa
    76081 aatttaacat ttggattgat aattgttatc gtttgcatta tcgttacgac gcaatcaaaa
    76141 aaggctgaca aatcagaggc tgttccggct ttctgggatg atcacctgca tacaaataag
    76201 tccaccgtga tgctgccgta cgcaagggga cgtaaagaag atgtgagcga tacccatttt
    76261 attttcgtag ttacctcatg gagatatgga atgtttaggt tgaacccttt cgtacgggtc
    76321 gggctgtgtt tgtccgctat ttcttgtgca tggcctgtgt tagcggtcga tgatgatggc
    76381 gaaacgatgg ttgtcactgc atcttccgtg gaacaaaatc ttaaagatgc acccgccagt
    76441 atcagcgtca ttacccagga agacctgcag cgaaaaccgg tacagaatct gaaggatgtc
    76501 ctcaaagaag tgcctggcgt acaactgacg aacgaagggg ataaccgtaa gggcgttagt
    76561 attcgtggtc tggacagcag ctacaccctg attcttgtcg acggtaaacg cgttaactcc
    76621 cgcaatgccg tcttccgcca caatgatttc gatctgaact ggatcccggt cgattccatc
    76681 gaacgtattg aagtggttcg tggcccgatg tcgtcgctat acggctccga tgcgctcggc
    76741 ggtgtagtga atatcatcac caaaaaaatc ggtcagaaat ggtcgggtac cgttaccgtc
    76801 gataccacca ttcaggaaca tcgcgatcgc ggtgatacct ataacggtca gttctttacc
    76861 agtggaccat taattgatgg tgtgctggga ataaaagctt acggcagcct ggcaaaacgt
    76921 gaaaaggatg acccgcaaaa ctcaacgacc accgataccg gagaaacgcc gcgtattgaa
    76981 ggattctcca gccgcgacgg caatgtcgaa tttgcctgga caccgaatca aaatcacgat
    77041 tttactgccg gatacggttt cgaccgtcag gatcgtgatt ccgactcgct ggacaaaaac
    77101 cgcctggaac gccagaacta ctccgtcagc cataatgggc gttgggatta cggcaccagc
    77161 gaactgaaat actacggtga gaaagtcgag aacaaaaacc ctggcaacag cagcccgata
    77221 acttccgaaa gcaatacggt cgacggcaaa tacacgttgc cgctgacggc gattaatcag
    77281 tttctcacgg ttggcggtga atggcgtcac gacaaactta gcgatgcggt gaacctgacc
    77341 gggggaacca gctccaaaac gtctgccagc cagtacgcgc tgtttgtgga agatgaatgg
    77401 cggatcttcg agccgctggc gctgacgacc ggcgtgcgta tggacgatca cgaaacctac
    77461 ggtgaacact ggagtccgcg tgcctacctg gtttataacg ccaccgacac cgtaacggtg
    77521 aaagggggct gggcgacggc atttaaagca ccttctctgt tgcaacttag ccctgactgg
    77581 acgagcaatt cctgccgtgg cgcatgtaag attgtgggta gcccggatct gaaaccagaa
    77641 accagcgaaa gttgggagct ggggctttac tacatgggtg aagaaggctg gctggaaggg
    77701 gttgaatcca gcgttatcgt tttccgtaac gatgtgaaag atcgtatcag catcagccgt
    77761 acgtctgacg tcaacgctgc accgggctac caaaactttg ttggttttga gacgggcgct
    77821 aacggacggc gcataccggt atttagctac taca
//
LOCUS       DBKEKI010000003        65181 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.003, whole genome shotgun sequence.
ACCESSION   DBKEKI010000003 DBKEKI010000000
VERSION     DBKEKI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.003"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1434
                     /gene="gadC"
                     /locus_tag="ACUQPX_000175"
     CDS             <1..1434
                     /gene="gadC"
                     /locus_tag="ACUQPX_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HHV9054982.1"
                     /translation="SKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIFYYTFAAIMF
                     LVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYFPTVLTFGAV
                     SVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSKWCGIIGTII
                     PAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAYAGMEMNAVH
                     VNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLLVAYDMLFEW
                     AGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKTNRHGMGHHL
                     MFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFAGAIYLRHSQPDKK
                     RPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLVALFLLIPIL
                     IYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            1468..2277
                     /gene="murI"
                     /locus_tag="ACUQPX_000176"
     CDS             1468..2277
                     /gene="murI"
                     /locus_tag="ACUQPX_000176"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HHV9054983.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNTNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNPTINIFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNLFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(2396..3090)
                     /locus_tag="ACUQPX_000177"
     CDS             complement(join(2396..2844,2844..3090))
                     /locus_tag="ACUQPX_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element ISEc30 family transposase"
                     /protein_id="HHV9054984.1"
                     /translation="MARVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQLTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVTHADVA
                     FICELDEQWSYVGSKARQHWIWYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQCIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            3589..4605
                     /gene="umuC"
                     /locus_tag="ACUQPX_000178"
     CDS             3589..4605
                     /gene="umuC"
                     /locus_tag="ACUQPX_000178"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HHV9054985.1"
                     /translation="MPDNYRIQEVEPYSIDEMFIDLRGINHCISPEAFGHQLREQVKS
                     WTGLTMGVGIAPTKTLAKSAQWATKQWPQFSGVVALTAENRNWILKLLGLQPVGEVWG
                     VGRRLTEKLNALGINTALQLAQANTAFIRKNFSVILERTVRELNGESSISLEEAPPAK
                     QQIVCSRSFGERITDKDAMHQAVVQYAERAAEKLRGERQYCRQVTTFVRTSPFAVKEP
                     CYSNAAVEKLPLPTQDSRDIIAAACRALNHVWREGYRYMKAGVMLADFTPSGIAQPGL
                     FDEIQPRKNSEKLMKTLDELNQSGKGKVWFAGRGTAPEWQMKREMLSPAYTTRWTDLP
                     VAQL"
     gene            4825..5451
                     /locus_tag="ACUQPX_000179"
     CDS             4825..5451
                     /locus_tag="ACUQPX_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HHV9054986.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
     gene            5448..5750
                     /locus_tag="ACUQPX_000180"
     CDS             5448..5750
                     /locus_tag="ACUQPX_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9054987.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            complement(6253..7599)
                     /gene="yagA"
                     /locus_tag="ACUQPX_000181"
     CDS             complement(6253..7599)
                     /gene="yagA"
                     /locus_tag="ACUQPX_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HHV9054988.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMALHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRPRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYMRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
     gene            7953..8555
                     /locus_tag="ACUQPX_000182"
     CDS             7953..8555
                     /locus_tag="ACUQPX_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HHV9054989.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            8793..9059
                     /locus_tag="ACUQPX_000183"
     CDS             8793..9059
                     /locus_tag="ACUQPX_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9054990.1"
                     /translation="MIIGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(9250..9531)
                     /locus_tag="ACUQPX_000184"
     CDS             complement(9250..9531)
                     /locus_tag="ACUQPX_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9054991.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKSPE"
     gene            complement(9637..9912)
                     /locus_tag="ACUQPX_000185"
     CDS             complement(9637..9912)
                     /locus_tag="ACUQPX_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HHV9054992.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(9912..10196)
                     /locus_tag="ACUQPX_000186"
     CDS             complement(9912..10196)
                     /locus_tag="ACUQPX_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HHV9054993.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            complement(11099..11971)
                     /gene="repA"
                     /locus_tag="ACUQPX_000187"
     CDS             complement(11099..11971)
                     /gene="repA"
                     /locus_tag="ACUQPX_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HHV9054994.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENRLRKKQGMDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            12810..13316
                     /locus_tag="ACUQPX_000188"
     CDS             12810..13316
                     /locus_tag="ACUQPX_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005082529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HHV9054995.1"
                     /translation="MNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILK
                     KVRRTDKVCGYRRHIHAVFPGYFFLKADPESHSFTHLRRHSAFLDFVKMAGEIKTVRE
                     DIVQSLMKVYPDPALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHL
                     VSDDGALT"
     gene            13457..14119
                     /locus_tag="ACUQPX_000189"
     CDS             13457..14119
                     /locus_tag="ACUQPX_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HHV9054996.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALATC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTGDTFSTTGRMIRPVAFSLIHP
                     ATINTSEVEEIFRDEGFMLAHGRECSLNGSGRNMLSRILHVGHSGLAEEEWGMDNSLL
                     LH"
     gene            14392..15006
                     /locus_tag="ACUQPX_000190"
     CDS             14392..15006
                     /locus_tag="ACUQPX_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HHV9054997.1"
                     /translation="MTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTPDL
                     PEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAPLC
                     LLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTDRF
                     GQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            15033..16727
                     /locus_tag="ACUQPX_000191"
     CDS             15033..16727
                     /locus_tag="ACUQPX_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0016600 - flotillin complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HHV9054998.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTARVHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            17166..17501
                     /locus_tag="ACUQPX_000192"
     CDS             17166..17501
                     /locus_tag="ACUQPX_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9054999.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFTIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GHVSITGKK"
     gene            17728..18798
                     /locus_tag="ACUQPX_000193"
     CDS             17728..18798
                     /locus_tag="ACUQPX_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HHV9055000.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREAHQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSTATP
                     AGPTPVTPAVSVSRAQTTTGTSGTPESRTTTPAPVATKPVVPPQQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPAE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            18802..19239
                     /gene="pilM"
                     /locus_tag="ACUQPX_000194"
     CDS             18802..19239
                     /gene="pilM"
                     /locus_tag="ACUQPX_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HHV9055001.1"
                     /translation="MGWVTGFIMVIMAIGNFWFHSHLSRTVHHQQTAEITQQAADFIR
                     YMNAINDYLYQHPERRAAGGQLTSAQLGLPATKNVSHLISQQRVFVWAKEKPGLMGAL
                     LEQSGDSALLARVENGRLLDTRGRRISITLPAVIPDQVIIWMN"
     gene            19271..20890
                     /locus_tag="ACUQPX_000195"
     CDS             19271..20890
                     /locus_tag="ACUQPX_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HHV9055002.1"
                     /translation="MNKKLLAIMIAATLLQGCTSFRQAENTRKDAVAEQQRAREMIAE
                     LRNRTPVVKESPRQWINPRPVTDKNPGNAVPGCPIIINTRQSITLQQVAQRISQNCHI
                     PVRITPDVQAYLSVTTSGSTQQIQGTVPPPDTGGMVPLAAIGSSDTTLPRLQGNTASL
                     PELKASGLTSLLNAVASRLGISWRYDGSHITFYYLDTRTFPVTYMDSQVAYNATVVSG
                     TMSSNGSSGGTASDAISGDASNTQTTTVDMKSALYQDVKNAVSSMLTPGIGRMFMSTG
                     FITVTDTPQVLETVRTFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDTVTGGVSIVNGKLTGSKAFLKALSSQGDVSVVTRNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            20911..22206
                     /gene="pilO2"
                     /locus_tag="ACUQPX_000196"
     CDS             20911..22206
                     /gene="pilO2"
                     /locus_tag="ACUQPX_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HHV9055003.1"
                     /translation="MTDISPTERQVFPLTAGRNVFLAGMDWKTLPASHKNPRTFARSL
                     GAVRYISCEYLSTEDTDRHIMVAAVSQNTLPKGSRRYFSLAMLILPLLEPGGYAIVEL
                     SQANDTELYGFVSAVDGILVSDLVGTREEIREAREIFLTINSAPEHGWTCYEPPSFNG
                     PDGRGPLPLETLTGAGKYPAEARLHPVSRGPQLIPVLLILTLLGTAWYGWQYYERLKA
                     EKAALAEAAQKAAEERITPPWLSMPETGDFIRGCSDVWSRLPVSVAGWRYSLAECAAT
                     GNSGNLRASYTNIAGTTVTAFSQRVREIFSIRPFMVMPDGNSGGFALPVTFSMPETPV
                     AVEALPENTLLQERLTTLARSMQLKMDWQEVSNSFTDEDGNTIQPPWKEYDLQILTTL
                     PAHQVAEHFSEPSVRFISVTRQLEEGRFRYQFTGKYYVQ"
     gene            22196..22654
                     /gene="pilP"
                     /locus_tag="ACUQPX_000197"
     CDS             22196..22654
                     /gene="pilP"
                     /locus_tag="ACUQPX_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HHV9055004.1"
                     /translation="MSSKTKALLLSVLILPTTVAASTISNMQQNNRQLQVEQIQEETA
                     LYEAMLAREKVRHELRTLEQGEPAEPVHSQESSSAVTTDTPAPITGIPRLTEISGVHP
                     QITARIQLPGGQTADVKEGQHIPGTTFRVEKITTDSVVLEQNGIRTTLQP"
     gene            22758..24266
                     /locus_tag="ACUQPX_000198"
     CDS             22758..24266
                     /locus_tag="ACUQPX_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HHV9055005.1"
                     /translation="MLAPADQPPDDILDYVVVNTDKTGQIHVRVVEGKKHLRAVQDYL
                     TRLCSRYPGKVSPFEFTTLDVIARLRQETVISGVENGINPVQQKMLGYISHAATLSAS
                     DLHITPGRDSTDFTYLEARVHGELELLDIVRKDEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAERFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQHIPTFSMLGYHPEQE
                     RAVRRMLQRPEGIVILSGPTGSGKSTTLRTASAAYLEQYGFNDTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSVKSALRLDPDAILNGEIRDHASAITAIKAAMTG
                     HLMLTTLHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQVICPHCRLPWHEV
                     ESSRTDEERRLVENFCQPDAVYLRNHNGCPHCWRGVNGRTVIAEVISPDAKFFQIYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            24268..25362
                     /locus_tag="ACUQPX_000199"
     CDS             24268..25362
                     /locus_tag="ACUQPX_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HHV9055006.1"
                     /translation="MKILERLNNTELELSGLNRWLGKKTFRRTTFYESLAGMIRDGTP
                     VMRALEFICDVETDFGKKKGQSGLYFLATDCIASIRSSGQLSPALKDWVPKDEIALIR
                     NGEERGDIAEAMFQVVKTAKGRQEMISSLVSVCLYPLILLTLCVVNMYNVHTRVIPMI
                     SAFAPEERWSVQMVILAKSSGLVIGYGLWLLAGLLLLAILIRMSFSLYTGPRRRYLDR
                     IPPWSLYQTFHGVNYLFNISSMLQINIPLSHALTRIEKHAEHNRWLKSRISAIRKNVL
                     SGQSLALAMKNCGYDFPSKSCVNNLLLNSEREDSVASMALYADRWLEEAKKNVRKSGI
                     IITAISGLLVFLFIINMVSAIYGISDMLKQ"
     gene            25424..25960
                     /locus_tag="ACUQPX_000200"
     CDS             25424..25960
                     /locus_tag="ACUQPX_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HHV9055007.1"
                     /translation="MSVITSSYSEHNIRHLQNDDKGMTLLEVLGVLVVAAIVIGAVMG
                     LMSDTLSSSDNQKELKNLQTIATKMKAQKFQGQYTGTDYVKILTESGGLPADMIAGGN
                     KAKNAWGGAVTIKVSSDKYSYVIESSNVPKKNCIDLVTSLRSSSMFTKINGNVTNKVD
                     PSTVCNADKTTIKLETNS"
     gene            26005..26490
                     /locus_tag="ACUQPX_000201"
     CDS             26005..26490
                     /locus_tag="ACUQPX_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HHV9055008.1"
                     /translation="MNRLFILTVIFSLLCSLPSAQASPQDKQYDRCFITAGKRYSIDP
                     GLLKIIARRESSLNPRAINHNRNDRGKIISTDYGIMQINSTHIPGLKKMGIIRSEKDL
                     LNNVCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKSNTHRRMKYARQIYSAYMQG
                     R"
     gene            26506..27132
                     /locus_tag="ACUQPX_000202"
     CDS             26506..27132
                     /locus_tag="ACUQPX_000202"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HHV9055009.1"
                     /translation="MLQIIPALLVAITVVIQCLAVQMSRYRLSRMNARPPHHGLALMS
                     GMLAAAAIILRLYQNDSAVGVLFMTIMTGCMTLMTLTDAASCRLPRPFTIMYLISGSA
                     FRLWQGDWLTGLCSAGFWFLLLLLFRQYTSYRRKTETIGLGDVFLIAGIAIWSPPGDI
                     PWIVATAAGGALLYLWCNRQRRITRELPFGPFLCASQYAFTFYPGGLW"
     gene            27150..28511
                     /gene="pilV"
                     /locus_tag="ACUQPX_000203"
     CDS             27150..28511
                     /gene="pilV"
                     /locus_tag="ACUQPX_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HHV9055010.1"
                     /translation="MQKDNDKGFALLEILGGLVVISLLMPLFWSYIEDYLNEMRNQSA
                     AFHADAYNTAARTYIADNNARLHSGTLPATFTADELIRKGYLKGLNRSPFGQSYTTGI
                     RRNTSTGRLEALTCSTGGENIKDDALRSIASLLPGLGGFIGKNGTATGVFGGWTDKPG
                     DYGLSCNGGHIAIVMMGDDLQESDRLYRFQVPGRPELNQMNTAINMGGNNLNNAGNVN
                     GQSATLKGDVTSENGWLITKNDKGWKNITYGGGFTMTDSQWIRAVGGKGIITTGEIKG
                     GKVSGGTVRSDGRLSTGEYLQLDKTAVANTKCSPDGLVGRDSKGAILSCQSGVWVGFE
                     SYPVGSPIPWPSATPPQGYLLMNGQSFSCSRYPQLARAYPSCKLPDLRGVFIRGWDNG
                     KGLDGDRNRQLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYIV
                     KAS"
     gene            28625..28819
                     /locus_tag="ACUQPX_000204"
                     /pseudo
     CDS             28625..28819
                     /locus_tag="ACUQPX_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            28984..29805
                     /locus_tag="ACUQPX_000205"
     CDS             28984..29805
                     /locus_tag="ACUQPX_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HHV9055011.1"
                     /translation="MKTDSTGIAARMMLSLDRERICECLLSHRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGEILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PATQEIFCRIISARENMDIILWHGFPFAGTHSGEYCGVLLRQKKEINNTIIYPVLSLY
                     PARMDIEKEGWFQMARKISPDIPVRAVTLTPAQAEYLITGKILPTALFRVPLSPW"
     gene            29907..31109
                     /locus_tag="ACUQPX_000206"
     CDS             29907..31109
                     /locus_tag="ACUQPX_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HHV9055012.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIADNADKISDDWKAFDRAVD
                     SHSGVPQTAARLKERLQDFRNTHASAQAGVSAVAALPGDTLAAALMLKTLGTVSVDGK
                     VSDADLNYLESIADSGSQDVDKNRLTSQAFARAALITDVGVALATELETAGQKWSLGF
                     TPKFQRVDLFNYNTLIKNYDSSAFKGDRYHNTQNGINADIGASMDLDDNWTLGLVAQN
                     LIPRSIETKEVHGVTETFRIRPQVTTGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AVGAEFNAWKWAQLRTGYRQNLASNNGSAFTAGIGLSPFDVIHIDVAGLVGTDNNYGA
                     IAQLQFTF"
     gene            31213..31671
                     /locus_tag="ACUQPX_000207"
     CDS             31213..31671
                     /locus_tag="ACUQPX_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238171.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HHV9055013.1"
                     /translation="MQKKRITLAGGVAMLCLSGCHLPPDNTAPARKYGAASEVAVTRH
                     AQYQLWEQGTYSAALPSRQPGGSLQAHSGRVSFDWEGDALELLSELARLRGQQFSYSG
                     VRLPLPVSLHVRDMTFADALRLTEAQTAWRAVLSQSAGRLHLSYMPTEKK"
     gene            31668..32504
                     /locus_tag="ACUQPX_000208"
     CDS             31668..32504
                     /locus_tag="ACUQPX_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HHV9055014.1"
                     /translation="MKKQVCLLLSGLLLAGCAGQHHRPPAGMAGNLPPPDLRAWLTPT
                     DNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISR
                     EGWLPPVIDEAQDVAHIQPDQIRTASRVWTILRPERFVSNPPGWRDWLLRGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRDNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQRQTVTGNGRKLITGDHVRRLKTHAEFTLQKSRWRPVVSTEGAPDEI
                     TR"
     gene            32488..33636
                     /gene="traJ"
                     /locus_tag="ACUQPX_000209"
     CDS             32488..33636
                     /gene="traJ"
                     /locus_tag="ACUQPX_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HHV9055015.1"
                     /translation="MKLPDEFGTFPFARFTAEEFRYFFAWCARHRVSDIDLTGGSPVS
                     VNRFGRRVRSSAHPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKVISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRYIQTHFPDRKTVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRDTLSRQS
                     HATWGHYIDEIIRKEKRRIRDQVLTMYQTGSIEASEAALFIPAGEFPK"
     gene            33633..33923
                     /gene="icmT"
                     /locus_tag="ACUQPX_000210"
     CDS             33633..33923
                     /gene="icmT"
                     /locus_tag="ACUQPX_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IcmT/TraK family protein"
                     /protein_id="HHV9055016.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFVLLGYSGWTVRTLCRRLYSLVQGKQASGRPWWYRHRTELPREWRGTE"
     gene            33987..38048
                     /locus_tag="ACUQPX_000211"
     CDS             33987..38048
                     /locus_tag="ACUQPX_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HHV9055017.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYRAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQAGYARSYINRLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDASNMIAVAENARQIWTDSPFVFCADNDHQREINKGVFSATKAAEVTN
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDNFQNAMNAQLKHIGIPTPNSDTADHREA
                     VVIDNLIFTPVKDEKPQMSPENRQSTAPETELATQDTPYDTHSDDTENQAYEDDYAAY
                     ASLQSVTAETLSPDKPLENIASAADTGTENTSVPTAGDQEDLNTDATGTDTAHNSDTA
                     ETGTLPEPAGMQSVQQATTEPTAEDKPHENVVSAADTREENASVATADTGEENASATT
                     ADNQSTQAEEHPRSEDTPETSLSDAAGTQHTTTTQSEEQVQVSMASADDWSAFAEDLN
                     ADTPGAPTVHNTDNAETNTVSEPAGMRPVQQVTTELTAEDKAQEKSVASAAETGAEHS
                     SDITDDNRNAPAEELNADATVAVHNTGTAETASPSEPAETQPVRQATAEPTAEDKLQE
                     ERLVSATETSTANEAARPETPSLFSRLRRFLTGVKSRTSEPSVIQEKKPTEAETATEE
                     VAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYSV
                     DGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIV
                     EYDIKVRMKLPAQRAALEKLRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVS
                     PKAEAEQTAGSTAETPHPEAPVTAKAEAPRADRPEHPAAPSSAPEKAPVSAQPQQPDT
                     PVEEPPGKLSPGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVK
                     NHQKGDPVTLTLQARETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFP
                     MATFSQVMLQMQQHWPQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAP
                     NELIPAIASADKDNRQLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINAL
                     TDNGLQFAGYGEAVNHDENNHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSL
                     GFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGR"
     gene            38065..38415
                     /locus_tag="ACUQPX_000212"
     CDS             38065..38415
                     /locus_tag="ACUQPX_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HHV9055018.1"
                     /translation="MNMKKTLLVILTGITLASPVAGAATCRAGAAAEAGSRAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDISITLPQFPSLDKLLRDLENQVCDAVVDRVND
                     HLPGNIDPWKDYNL"
     gene            38427..39122
                     /locus_tag="ACUQPX_000213"
     CDS             38427..39122
                     /locus_tag="ACUQPX_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HHV9055019.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLYSGAALCISLVANGIQYWHATGVTREYFATDNGRLVRLAPTSQPAWSQSDVMNFGS
                     MTLSEAFNLDFVHYRKQITTQAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTTTIG
                     AGTLVNQGQMENGVWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAAAGI"
     gene            39133..40107
                     /locus_tag="ACUQPX_000214"
     CDS             39133..40107
                     /locus_tag="ACUQPX_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HHV9055020.1"
                     /translation="MHIHHLIGALLIVCGTVTAADNPGWKNARSPQTGGKTAQATGNT
                     PAGGPPPAAQLPGNLPPPGAALVQEGASLDSPLNASEIRELRGIMADNERAISAPLTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDVNGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTTTLSQEIDSRLDLRIPR
                     QGPASPVVSIPSEKIALHDATLQAFLDGVPPDDPAIRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            40111..41448
                     /gene="traO"
                     /locus_tag="ACUQPX_000215"
     CDS             40111..41448
                     /gene="traO"
                     /locus_tag="ACUQPX_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HHV9055021.1"
                     /translation="MSAEQEQDTAAKSGKKLSALLVMGGVIIIGGGYLLLTWLSPENA
                     ASLSSVNINSTTSGGGRNVMETQHYRDLLRADNERGAAEALRNNETFIASLPRGLDTD
                     TGNDQKRDNPPDDQSGTSGQNASPPQEDQTAREKRREALQKLLARINTQHQDARPPVV
                     AAAMWSTTGTPGTAPHASPPGTVTSQHPRLQETSLATPAARPGIQLIPALTRVPAYID
                     TEVDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGQGMDIHFDRMMWRGMFLKVNAYA
                     LNERTGMSTVASDVNHRWFKHIVLPSVLGGVGNVGSLYKDANTQILQGNYGSITGRVG
                     MPDGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDIST
                     PSSGTAPSESPSSGRPLTQAEAEHQASDRIRAAVLRKQNEMQQRYGIQQESYP"
     gene            41445..42158
                     /gene="traP"
                     /locus_tag="ACUQPX_000216"
     CDS             41445..42158
                     /gene="traP"
                     /locus_tag="ACUQPX_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HHV9055022.1"
                     /translation="MKNEHEDPGSFGETTAPASGKSFLKRQYMGYSLPVWAGCALIIL
                     VAIWYKFLRTPSEPPADAGFAVTPPAAESWAEPMTMTESGSGNNMPAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKDSITTLSNAIQKDEAWAQETRQLLEETRKQVHTL
                     QMQGALPAPTGNNRQPAKTLPSPLQGMKIMSVEKGMAWIRWQGSAWAVREGDSLGNIT
                     ITRIDPSSRAVITSAGTLR"
     gene            42155..42685
                     /gene="traQ"
                     /locus_tag="ACUQPX_000217"
     CDS             42155..42685
                     /gene="traQ"
                     /locus_tag="ACUQPX_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HHV9055023.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIFICGMLVSLPSMINAGGTQMGFRADSFAPIAYVQPGSFGAA
                     AGAVNAILSLAKLAGVGFVMNGISIWRKSLLDGHTALSASESISKGNVKFVAGVLLVF
                     NDRVIDATLASLGIAF"
     gene            42732..43130
                     /locus_tag="ACUQPX_000218"
     CDS             42732..43130
                     /locus_tag="ACUQPX_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HHV9055024.1"
                     /translation="MSRLRAMAARLRAASVYVQLCLMACRDRLVRLLLLLPVMLLPHT
                     AMANTLFDMIKTVGDGARGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGVCLAVVPELMGSGQKTLGLSPVSIS"
     gene            43187..43438
                     /locus_tag="ACUQPX_000219"
     CDS             43187..43438
                     /locus_tag="ACUQPX_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HHV9055025.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKAYEKDPATHRPGPGTMAFPAPATGHGIQR"
     gene            43353..44171
                     /locus_tag="ACUQPX_000220"
     CDS             43353..44171
                     /locus_tag="ACUQPX_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HHV9055026.1"
                     /translation="MKKTPPLIAPVRERWLFPLPQPDTVFSGEALLPPPVLSTEGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCQDTIVLLEKNLTRVMRLKKRPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESDLLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALQEIFSDNRQRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPEQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            44466..47510
                     /locus_tag="ACUQPX_000221"
     CDS             44466..47510
                     /locus_tag="ACUQPX_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHV9055027.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDQTAPGTLESLIAALAERMNTAW
                     KNSGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRVRKLAEHAPPARFAQDPWRWTLSALKIRHDALLD
                     TLEQALTHDSDGLLIRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGGD
                     TSVFHAPSLNLQLFTTQPETHGSLIQAGELWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPDGMKALGVKKTLLTYSSFIPPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PDHTRNLFDVMYGARKPVTPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISSLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHIAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSEERLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQHYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQEVGSLRARQI
                     LAEHFPRGSATSLIEHRARTHDSENVIHELAAELIRKQGYNL"
     gene            47510..48130
                     /locus_tag="ACUQPX_000222"
     CDS             47510..48130
                     /locus_tag="ACUQPX_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HHV9055028.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMSDNTWLWPDVPGTPALTAGDWPVMESDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESLSHRVPDILYPPKTEKKECKDNLQPHCLHSL"
     gene            48088..49293
                     /gene="traW"
                     /locus_tag="ACUQPX_000223"
     CDS             48088..49293
                     /gene="traW"
                     /locus_tag="ACUQPX_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HHV9055029.1"
                     /translation="MQRQSATTLLALLVGCAVIPATHAYSVNVVSSQPVTRQIMPELA
                     TIQGIMEEMVRMQTATGTAITQNSEKLATVIAQDGQATRQQMIFSNETHRLEEARKSF
                     SVPDSICSESASGIAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPVREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGDLPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            49290..49859
                     /gene="traX"
                     /locus_tag="ACUQPX_000224"
     CDS             49290..49859
                     /gene="traX"
                     /locus_tag="ACUQPX_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HHV9055030.1"
                     /translation="MKDDNSIDEKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     DQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVDELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPVITLIRILVCLLLMSTIAALCASKALIASYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            49934..52099
                     /locus_tag="ACUQPX_000225"
     CDS             49934..52099
                     /locus_tag="ACUQPX_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HHV9055031.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIATMGIVWFLFIGIRHAVRTGHQGSVFNN
                     GRDVVGILSVVSGFLMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMELCKHAMNTGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNKQSALAHFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPADNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLEDLANRAPTVSGVSSLGEAGDTEFLKEVKGAYQ
                     AQLQNSAYTPALGTATSASEYHASKTQDPQSAILKLIGFNLTRMTNWTATYLSGTGTT
                     TNQLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALYAAALM
                     NVQINSVTGVFSMAGFLMIYARACTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNMRRTPGVREEKLKNINRDDKDGIKQ"
     gene            52187..52840
                     /gene="excA"
                     /locus_tag="ACUQPX_000226"
     CDS             52187..52840
                     /gene="excA"
                     /locus_tag="ACUQPX_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HHV9055032.1"
                     /translation="MNTTKSRYPSWWAYYYLIRTAYFIFGVPFLLLCSLGFTGMMLTE
                     PYIETDIQAYLYTYGSWLLLILPSLWLYMKTRAKKKKVQQVVQQIKESSPFAPTSDYE
                     QLSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVS
                     LPMIPLEISGISSRTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            53114..54700
                     /locus_tag="ACUQPX_000227"
     CDS             53114..54700
                     /locus_tag="ACUQPX_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HHV9055033.1"
                     /translation="MSESRSNFPARHGTPWNTEELQRLLVSIAEGVSLKKLTQTHQRS
                     SGGIRGAIKRLLPSEFLEFNDINTVNDLSNYFNKIPRQEHNSIINNLISLSTAKTIAH
                     DKVEIQKNSFKQNTPLPLCQPNTDFKDYIADEHDVIMLIHSAIESLSNKRHQYILLMR
                     LGTDDSPHSLADIAVKLGISRERVRQLQERALGLLAAKSRYEGTPGACLKALINSIGN
                     SPYDIAVWIYRIVHRDFVTSSELAAKFIIFAAGIPKARRDEIKNSLSLISQLQKQKLR
                     ERRAELAHAHAQERARIKKERVEFIFSKWLDNTHWPKYIAPPPPVEQLCSQRAVNDTD
                     ISGFFYSQKLGRTVQYESGLEFKIMNLLECCDQVLFYQEQPFSIPYCFKNKSKKYYPD
                     LLIATVDGRCLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSLRQLEE
                     HAISTNTWKLIEAELKTSRILAWREMMELRTQYEIHRLDLIAYIIQSGAEVDNFYRIT
                     PKISNNTSNQRQPNESDCHN"
     gene            complement(54838..54990)
                     /locus_tag="ACUQPX_000228"
     CDS             complement(54838..54990)
                     /locus_tag="ACUQPX_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HHV9055034.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRIEQGNTVFVATL
                     AYEVKR"
     gene            55320..55562
                     /locus_tag="ACUQPX_000229"
     CDS             55320..55562
                     /locus_tag="ACUQPX_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055035.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALTMLSTLCYNLM
                     MTCGIHSMDSQMLNGHGISMQLQGIDIAEKPGKNIH"
     gene            55853..56221
                     /locus_tag="ACUQPX_000230"
     CDS             55853..56221
                     /locus_tag="ACUQPX_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055036.1"
                     /translation="MKDNNTTATPETWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
     gene            56225..56440
                     /locus_tag="ACUQPX_000231"
     CDS             56225..56440
                     /locus_tag="ACUQPX_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055037.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(56638..57261)
                     /locus_tag="ACUQPX_000232"
     CDS             complement(56638..57261)
                     /locus_tag="ACUQPX_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9055038.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWEDVMRECRDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYSLATRIRRVDSYGMTTRCFTDIAGQVKAPTARLKLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRVVSGYSL"
     gene            57420..57887
                     /locus_tag="ACUQPX_000233"
     CDS             57420..57887
                     /locus_tag="ACUQPX_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HHV9055039.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVR
                     VRLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            58037..58213
                     /locus_tag="ACUQPX_000234"
     CDS             58037..58213
                     /locus_tag="ACUQPX_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055040.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFDSR
                     EQAQEVQEGFRFLT"
     gene            complement(58276..58371)
                     /gene="dqlB"
                     /locus_tag="ACUQPX_000235"
     CDS             complement(58276..58371)
                     /gene="dqlB"
                     /locus_tag="ACUQPX_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HHV9055041.1"
                     /translation="MINLLIIVLRAVVAVANAVIALLELIRELVD"
     gene            complement(58960..59055)
                     /gene="dqlB"
                     /locus_tag="ACUQPX_000236"
     CDS             complement(58960..59055)
                     /gene="dqlB"
                     /locus_tag="ACUQPX_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HHV9055042.1"
                     /translation="MINLLIIVLRAVVAVANAVIAILELIRELVD"
     gene            complement(59108..59254)
                     /locus_tag="ACUQPX_000237"
     CDS             complement(59108..59254)
                     /locus_tag="ACUQPX_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055043.1"
                     /translation="MSGDLPSHQSIGTMDLCYSKTWPDKQVVLRLVLWPEEVSFQLTM
                     KELL"
     gene            59592..60935
                     /locus_tag="ACUQPX_000238"
     CDS             59592..60935
                     /locus_tag="ACUQPX_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HHV9055044.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFGQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEVFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGETTETTETTETTETTETTETTETTETTETTEKQHPADKEKEQ"
     gene            60932..62056
                     /locus_tag="ACUQPX_000239"
     CDS             60932..62056
                     /locus_tag="ACUQPX_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003756 - protein disulfide isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HHV9055045.1"
                     /translation="MTIHYTATLNNKHLSLIALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            62037..64340
                     /gene="trbC"
                     /locus_tag="ACUQPX_000240"
     CDS             62037..64340
                     /gene="trbC"
                     /locus_tag="ACUQPX_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HHV9055046.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADTGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
BASE COUNT        16669 a        17545 c        16142 g        14825 t
ORIGIN      
        1 agtaagaaaa tgacagtggg gacactcgct atcatgaata taactgctgt agtaagtcta
       61 cgatttatac cttctgaagc agaatacggc ttaggtgcca ttttttacta tacctttgct
      121 gcaattatgt ttttagtacc tgttgccttt gttgcggctg agctagctac aacatatcca
      181 caaaaagggg gggcatttcg atggatagga gaagcatttg gggctcgctg gggttttgta
      241 ggaatgctta tgacctggct acaagtcatc ccctactttc caacagtact gacatttggt
      301 gcagtctcgg ttgcatttat tgatgttgat atgggggtgg cagaatcaat tgccagtaat
      361 aaatggtata ttttattttt cgtactattt gtttactggg gagctatttg tgttgcatta
      421 cgtggcgttg gtattttctc gcaggtatct aagtggtgcg gtattatagg gacaattatt
      481 ccagctatta tccttgttat tcttggtttc tcctatctgt ggttcagcgg taaaccagcg
      541 gtgatcccat taggttgggg tgatcttttc cctaatatag ctaacttcca gaatattgtt
      601 cttgccgcca gcatatttct tgcttatgca gggatggaaa tgaatgcggt ccatgttaac
      661 gatttagata atccaactaa aaaataccca attgcaatta ccatcgcttc tataggtact
      721 attgcaatat tcctgctcag tactttaggg gtcgccttta ttattcctac agataaaatt
      781 agtctaactc aaagtttgtt ggttgcctac gatatgctgt tcgaatgggc tggagtgcca
      841 tggcttggct ctgtgatggc atttatgcta gcaattggcg tactgggtgg ggtggttact
      901 tggattgcag ggcctaatac aggtgtatta gcgatcgcga aagctggtta tttacctaag
      961 tttttccaaa aaaccaatcg tcatggaatg gggcaccatc tgatgtttgt acaaggtata
     1021 attgtctcag ttctttctgt tacttttgtg ataatgcctt cagttcaagc agcttttcag
     1081 attttatcac agttaacagt tatgctctat ttagttatgt atatgctcat gttcgctggt
     1141 gctatctatc tacgacattc tcaacctgat aaaaaacgcc catatcgagc ccctgcttta
     1201 ttcttctggg caacacttgg attcttaggg tcttttctgg cttttgtatt aagttttatt
     1261 ccgcccgagc aaatcccaac tggtagtcca accagctatg ttctttattt gttagtatta
     1321 gttgccctat ttttattaat tccaatatta atttacgcct tccgaaaatc tgactggaaa
     1381 gatccaaact ctgatttcga accattttct tgggaaaaaa gaactagtaa ttaaattttc
     1441 aataagctac aaaagatgag gttaaatatg tttagtgctg aatctccaat tggtgtaata
     1501 gactcaggtg taggtggatt gacatcggta aaggaaataa ttaatttgct tcctggcgaa
     1561 gatattatct attgtggaga taatacgaat gcaccttatg gcaaccgttc tgccgatgat
     1621 atcatcagtt tgactaaaaa aatgctgact tttttacagt caaggaatgt caaactagtt
     1681 gctgttgcat gtaatactat atcctcaact ttggaatccg aagaatacag cggatatgct
     1741 aagagctttc cgtttcctat cttatccatc attgaacctg cagtagaaga tgtgattcgc
     1801 cagcagtata aaaatgtggg tattatcgct accgagttta ctattaaaac ggggtgtcac
     1861 aaagagctaa taaaaaaatt gaacccaaca ataaatatat ttggagaacc aagcaaaaat
     1921 cttgcaatgt taattgagga aggtaatctt aatgctcctg caatattgaa tgatatcaaa
     1981 aaacatgtta accatttaat ttccctccat ccagtaaatg aaattattct cggctgtact
     2041 cactatccaa ttgttcagaa tttatttgaa gcagttgcac cggatataaa atttattaat
     2101 cctgcacatg atcaggctat ctctattaaa aatcacttgg gacagctaaa tttattaaac
     2161 tcctcaaata taggaacttt gcaaataaat actagtggaa gtatggagat ctacaaaacg
     2221 gtactagggg aattgagcat tacgaaacct catacattca gtatcaggca gttttaaatt
     2281 tatccggtgc accgtaaaac cccgtccttc tgaactgcac cccaaaagtt ggacaccaac
     2341 tgagtaaggt gcattttatg ggcaaaccca gttacacact gggtagtgca tccaattagt
     2401 agaacatgtg tttttcgata aacgctccga tcactttttc gtggatctca actgagcgtg
     2461 agaagcagat tgttttacga cccaaccgct taatgcgggt gcgtagcgtc agattattac
     2521 gctcaatgca ttgggtgaat attttgccgg tcagatgctt attcttcggc acctcccggc
     2581 catagctgcc ccagtcatcg ctggtcagca tgccgatgtt gaagggtgta agcagtgcca
     2641 gtagctcccg gcacgtttga tcggttcggg gaccaaaagt gtaggccagt acaccgcctg
     2701 ttttggtgtt gtacgcgtac cagatccagt gttgccgggc tttactgcca acgtagctcc
     2761 attgctcatc aagctcgcag ataaacgcca catcagcatg ggtaacaggc gaagaagtta
     2821 ttcgctttgg cgcgagtttt ttaaagtccg gatgacggtg ttactaccaa ttttcagtgt
     2881 cctggcggta tcgcgaaccc cggcaccatt aaaggccatt tcagtgatca gctctttcat
     2941 acccggctta cgtgcttgat aagtataagt gagctgaaac acacggtggc agtcacggca
     3001 gcgaagtctg tcacggcctt tagggttctg accatggcgg taaacctgag ctgactgaca
     3061 acggggacaa tgaatattaa ctctggccat gagataacct caaaaacccg tattatacat
     3121 cagattcaac taattagagg catcacctat ccagcattat ataacatact aacaataact
     3181 aaattataat aatcacaact tataatcaaa taataaacgc ataaaatcaa caatgatttt
     3241 ttctggttat atttatctaa aaaaacggaa tattatgtaa cttattattt tctaagcgta
     3301 aatttattat gtggtcagac taacaaactt gcgtaccacc cgaagaatat gcgaaaattt
     3361 aagtattctt tcttttttta ttttttcatt ttctcttttc ttctctttcc ggttctcttc
     3421 ttttctggtt ttggtttttt cctttttatt tttattcttt cttcagggtt gtgctaaaac
     3481 accaaaacat ttttcaccac aaaagcatcc aatcatcttg ccagaccaat caccttaacc
     3541 ggatacactg ccggatataa tcccactaac acccagccac cccgcaccat tcctgataac
     3601 taccgtattc aggaagttga gccctactca attgatgaaa tgttcattga tttgcggggg
     3661 ataaatcatt gcatctctcc ggaggctttt ggtcatcagc tcagggaaca ggtaaagagc
     3721 tggacaggac tcaccatggg ggtgggcatt gcgcctacaa aaacgctggc taaaagtgca
     3781 cagtgggcaa caaagcaatg gccacagttt tccggtgtgg tcgcgctgac ggcagaaaac
     3841 cgtaattgga tcttgaagct actggggctg cagccagttg gtgaggtctg gggagtagga
     3901 cgcagactga cggaaaagct gaatgcgctg ggtattaaca cagcactgca actggcgcag
     3961 gctaacacgg cattcatccg gaaaaacttc agcgtcattc ttgagcgtac ggtacgcgaa
     4021 ctcaacggcg agtccagcat atccctggaa gaagcaccac cggcaaaaca gcagattgtc
     4081 tgtagtcgca gttttggtga acgaatcaca gacaaagatg ccatgcacca ggctgttgtt
     4141 cagtatgcag agcgggccgc agagaaacta cgtggggagc gtcagtattg ccggcaggtg
     4201 acgacatttg tacggacatc cccctttgca gtaaaagaac cctgttacag caatgccgct
     4261 gtggaaaagc ttccattgcc cacacaggac agccgggaca ttattgccgc agcatgcaga
     4321 gcgttaaacc atgtctggcg tgaagggtac cgctatatga aggcaggtgt catgctggct
     4381 gatttcacac catcgggtat agcgcagccg ggattatttg atgaaatcca gccccgtaaa
     4441 aacagtgaaa agttaatgaa aacactcgat gaactgaacc agtcgggaaa agggaaagtg
     4501 tggtttgcgg ggcgaggaac cgcccctgaa tggcaaatga aacgggaaat gctttctcct
     4561 gcgtatacca ccagatggac agatttgcca gtagcccagt tatgaatatg taatccaccc
     4621 ataatgttgt tatcctgtta tcctgttatc ttgttatctt gttatcttgt tatcttgtta
     4681 tcttgttatc ttgttatctt aattaatcta taaatcaaat acttaacaac ttgctaagtt
     4741 gttaacataa caaaaataat tgcatgattc gcgttcagat ggcaatatta agttattaac
     4801 ttaacagctt aatatgaggc tataatgata atagtattag gtagtcaaaa gggtggggta
     4861 ggtaaaagta cactggcggt atctatagct gcgtatctga tgtcactagg caatagagtg
     4921 attattgtcg acgcagatga ccagaaatca gtattaacct ggtacaacaa tcgccctgaa
     4981 gatctccctc atattccggt tacaggcgcg acaggtaaca tcaaagccat gttaaaggaa
     5041 cacgaaaaat cctatgattt tgttattgca gactgtgctg gccgtgatag cgcagaaatg
     5101 cgtagtggat tgatggccgc agatgtattc atttctcctt tgcgcccatc tcagatggac
     5161 ctggatgttg tgcctcatac atgctccgtt tttacagcag caaaagattt taatgaggat
     5221 gttcggggat atctcgtttt aaacatgacg cccacaaata tgtttgttaa tgaagccaat
     5281 gaggcagctg aagtacttaa ggattaccct gaaatgcatc tggcaaactc ccgggtatgt
     5341 gatcgtaaag cacatcgaga tgcttgggcc gaatcaatga caatttttga aacgcaaaat
     5401 gataaagccc agcaagaaat cgaagcgtta gtgaaagagg ttattctgtg agaaatagag
     5461 caaacacttc acctcgtgac gaggcagcat ttattaacgg tggcacggct ggccttgata
     5521 ataccaaaaa gtcatcagat aaatcatcgg ttgtaagcaa agcaaaacca gtgagtattt
     5581 ctttttttga agaaaaccta aaagacattg atgcccttat aacggctgag atggtttcag
     5641 gtaaaggcag agtgaatcgt tctgatgttg tcagggcggc ggtagaagca cttaaaacac
     5701 tatcaaaaac agaggtatca cgattgatag cagaagtaaa acaaagataa caccttgaga
     5761 taacaagata acaccttgtt atcttgttat cttgttatct tgttatcttc aatgtatttt
     5821 aggatgacct atattgaata gaacctaaaa caaaacaaat attaataggg gtccgcttgg
     5881 aaaagggaaa ttttcccacg ctaagagaat tgagttgaac gagtggttac atccaggcca
     5941 gctaactcac tgaaccccgt acagggtcag acattcggta tctccttcat cctctgagga
     6001 aaacaaatga attaactgct tacgtatatg gatacatata cagtattaat ttattaagga
     6061 gaaaatggca tgtcaagatt taatgatagt gattatcatt aacatgaagt aatgttacac
     6121 cgttcagaaa gcctgctgac gctgtggcag catcacagtc tgagtattct ctggtgcaat
     6181 acaaaaaccg gactttcccg taacaacaga caatgttacg ggaaaagtta cagaactgag
     6241 ccacaaaata tctcagtacc tgagatgcat aaaaacaccg tacatatgtt tctcaaaagg
     6301 gggatgatcg ttatccagcc atttcggtgg atcggtatgc agaacaccaa tctgcttcat
     6361 gccggtatgc tgcctggatt ttctcgcgca gtaatcactg tcttcatatc ccatcgtttc
     6421 gatgaccact ctggccgtct ttccgtcagg agcccttgca gtgacaatga aatccggcag
     6481 gacatatttc ttttcaccat taacataaac ttctgtatca aacaggggcc tttccagact
     6541 taattccggc gtacctggct ttcttttaag ccagctggcc acattcagaa gagcagtgag
     6601 cgtgtttcgt tccagtttgc tgtccacagg caccggacat gtcagttgat aaagagcatg
     6661 agcataccct tcactgcaga tgattctgcc atcggcactc cggcaaaaag caagaataac
     6721 ccagtaaggg ggacgtattc cgccctgcgc ttcaccatta atactgatcc cccgcacagg
     6781 tctgatgacc tgaatattac cgttcgccca gtgaatttcc acagcatcac gacagatgtg
     6841 ctgcgccata aaaatctgaa agaacatatg ttttcttctg gcaggccagt gtcgttctga
     6901 attctccagc gtttccatta accgctcctg agacctttca gacattccag gccggaaatg
     6961 gataatttca gataaggatc ttccccgcat atactcctgc tgcagtgtaa cctgccgtat
     7021 ggcatcaaga cattcacgat aattagtccg gatacgggga ttagccaggg aatccagttt
     7081 attcagccca gcatcgtcaa taagagacag gagtaaacgg gcaaggcgcg gacgtttctt
     7141 gcgaacggag gagctctcac cattatggta agcagagcgg gcctgcagtc tgataacaga
     7201 attactgtca tcaggtggta aaaagtttct gtaatttata cgggtgctaa cgggatgccg
     7261 gcttgctccg gaagatgttt cctcttcatt tccggataac tgacgataca tggggcattc
     7321 aggtgcatgc tcatgattaa aactggacag ataaagagta cccgtattgt ttttaagagc
     7381 agcaaaattc atggccggcg aatctcccgg aatacagtca cactgtaacc atgcatcata
     7441 ctcacgcatt tcccggagaa tatcattggc caggttatgt agtgccatgg ttgctggctt
     7501 tttcagtcca cagaccgtct ttactgcagt ctcttcctca ctgctaagct cccgaatcac
     7561 tttgccccgg ggattcttct ttacaagtat cattggcata atgaaagcac tccttatgta
     7621 catgcttaaa cagtaagtaa aatatacaaa gaacacccca tgaaacaaag gcagagatga
     7681 actgtgcgcc ccgatatctt cttctgatat cctttcaaat gaattgtttt tatttatatc
     7741 atgacaacat taaagtgaga gaatatatca accatgtttc tgtcagggtt cagtagaata
     7801 tcaattcgac tgtcacgcca taactgttac tacctcgagt ataaatactc agagaacgga
     7861 cttattagcc atgaaaaatc acctgatggg aaatttacta atttatgaat tgtgatatta
     7921 ttaaagagta aaaccagcca gccgaggtat ttatgggaaa aaatcaggaa gaaaggaaaa
     7981 ctcccgtcat tgtggtgaaa aaacgtcgta ctttttcgcc accgtcctta tccgaaaaaa
     8041 cagatattat cgcacctgta tttactgaac agacggcaga atcagccccc gccggaataa
     8101 attcatctgc cgtcgaaaca catatccccg aagcgccagc caggaagaag aaaaagaaaa
     8161 gacaccgttt tccacgtccc tcccactgga cccgtgagta tactcacgag tgcgtggaaa
     8221 aaataaaggc cctgtttcct catcttcgtg ctgaaggtgg tggatttatt cccctgaaaa
     8281 ttggcataaa caatgatatt tcggcatttc tggcagaaca cccggagaca gaactgacca
     8341 tggacgaatg gctctgtgca gtgtcctgca tcacatcccg ccgcgtatat ctgcagcgga
     8401 cagctgtcgc cggcgtccca cgttatggcc tggatggtca ccccaaaggg caggtctcag
     8461 actctgaagc acagagtgcc ggtcgcagac tggcgacatt agaacaaaag tggctcagga
     8521 tgcaggcaca gcaggagaat atttctgggc aataaacact cctgataaca ggtggacatt
     8581 acgtatcaga aaaaatggga agagaatgtt atgtgtgatt cagaagcgga gaagatttta
     8641 ttcatcctga ataaacatga acgggcatac agatgccagt accccgtata acttgataac
     8701 atcaggaatt ttattgataa acagcagtta acgaatagtg atactccgaa gaaaagcacg
     8761 gtcccaaatg aagataaaaa aggcataaga tgatgatcat aggaaatgga ataaacactg
     8821 taactgtcaa tggaaaagtg aaacacatca cagaactgga tgatataaca ctttgtttag
     8881 agtggacaaa actacgagag gaaaacaatc gtctttatga aattaataac caggcaaaca
     8941 gaggatggcg tggtttcatc ctaagattga taggggttaa cctgccagat aaaaggactg
     9001 agtttacaca acgtctctta ttgacgcgca aaatttcggg ttcggtcatg aaaaagtaac
     9061 atattctatg ctggaacaga ccaattttgc attcgccgtc caggagtttg aggggaaagt
     9121 agtcagtgtt tacgctcagt cagggaaagt gtgcaatgat aaacaacacg aaaggaatcg
     9181 tagtacatgg gcaccgcgat tcaatattca aggaaaacac aatacagcaa tgtacttgta
     9241 ctgtttctat tattctggcg atttcaataa aggcacttgc tcgcgctctg tcattttctg
     9301 aaactcttca tgctgcattt cgattttcct tctaagatca taactcgcat acctgttctt
     9361 tttatgtata tctatatcta tttttgtgac gagttcaaac cacaatgttc gcactcccca
     9421 ggcagcgtta tgaaacattt ccgcaacggc tatagagtct gtattaactg cttcggtcat
     9481 gtagtcgcta atacgttcag cggtaatttt cgttatttca tcgtaattca ttttatctcc
     9541 tacaagatta tttaatggaa tgaaaaatat ctggcctgat aataatattc cagaattaat
     9601 atctgcaggc tatgttaagt tgtccccaca cacaatctac ctgttttctc gtgtgtgcca
     9661 caggcgcaat acataaatag tctggccatt aagttcgtaa cggatttcgt actcgccagc
     9721 aaaaatccgt ctgacctccc tgggttcaaa ctgaaacaac tgttctccca tacgtggatg
     9781 agttaacaga atgaccggtg cctgtgtcag ggattgcacc gttctggcgg ccgcaggttt
     9841 actggccagc accagaaaat catataaccg cgccaaatca gaaagcgcct tactggtcca
     9901 cttcagttcc atcagcgtgg caccggtaac ggattgtcag tactgaggct gtccgcccag
     9961 gcctgtacag cctgatggtc gataacctgt ccggatgtca catcgtccag ggcttccagc
    10021 gtcaggcgat tacgctcctc ctcctgggca agccatgcag aaagcgcctg tttgataacc
    10081 cagccccggg agcgttccag cctggcggcc atctggtcga ctttatcagc cataggtaat
    10141 ggcacatgcg cggtaatgac ttttgttgct tgtgcggtat tgtttttcat ttgcatatcc
    10201 gattagtcgt taatcatatt taatcatagt gattactttc agacggaaca aggcaaataa
    10261 tgcgcgtcag cgcatgtaag gcggcatacg gtggttacat gtagcacagg tgtgaaaccg
    10321 ccgaagcccg gcgtaagccg gtacatattg atagatttca ccttacccat ccccagccct
    10381 gctaagccat acccgctttc agccatgaca gagcttctgt gcgcggtcgg agtggtcccg
    10441 acgagggttt acccgaagtc ggggcgtatc tccgcgttag cgggccgtca gggccgctta
    10501 cgagcgtgta ctcgatacgt ccagccagaa gactggcagc gatgacaatg tagttacaac
    10561 attcataatt aaaagcgatg ttgttcccgc cctttgggcg ggggcggcct cgcttttcag
    10621 ttatgagggg tgggcacggt gctgcgtttg tgtggtgttt ttattcctga tgtctttttg
    10681 ctgtggtttc agaacggtga ggttcggggc ccgcgcgtca gcgtgggagg tgcatatcag
    10741 tctgtccaca ggacaggcat tgaacaggtt ttctttttaa atggaagtaa ttaagtagtt
    10801 taaaggaaat acagtactgg agtttaaaag aaacattgca ccctgtaacg cctgcggctg
    10861 gcgctttata gcattaacgg ttgtaacctt atggagaagt cccttgcagt taaatgtgga
    10921 taaacagaat tacccgtctg tgagacgtgt tttgcgtgaa aaacaggggg agcatgatga
    10981 gccggaaggc agatgatgag gctgtttttt gtgtgctgtg ctgctttttt gtgcgtcgcc
    11041 gggcttcaga cgtgcggaag cctccggcgc aggccggatt attcagatga ggtcaaactc
    11101 agggtgaggc agtggccagc cgtgtgtagt tgttgttccg cgacattatc attcgttcct
    11161 tcacgcgacg gtcgatttcg cgcttcacgg cctccaggct tccgcggaaa cgcccttccg
    11221 cgatttcacg tgtcagctgc cgtttcacca gggtgacaat gtcctgacgg gtcctgcctg
    11281 catcgcggcg cgcacgggca cgtttcatgc cgtgggactt aagctctgcc tgataactgc
    11341 ggaaacgctc gcgcacaaaa cgccaggccc tcgctatcag ttcatccata cccagcgcat
    11401 ccatcccctg ctttttgcgc agcctgtttt cccattccac gcggctgcgg cgtgcggaag
    11461 ccactgcctc ttctgagata tcgagtgagt caaacagtgc cggcgtgaac gtgatgtcgg
    11521 tcgggatgtt acagccaatt gtcgggtcgt attccgtctg gtaggttatc agccccagct
    11581 cagacaggaa cttcagggca cgggtggcac gggtgatgga gagtttcccg gcctcagact
    11641 ccgtcgccag tccgcactca atggccagcg tggtgatgga gcactgcacg cggttggcca
    11701 gcgggtcata gtggaaacac agcccctgca gcagcgcatc aatcgcccgg cggcgcagca
    11761 ccggcggcat acgccggcgc aggccgcgcg aacgggcata cgccacatgc gcggcaaaat
    11821 caaaacggga ggtgaagccc acggcctttt ccatcagttt ttcgcagaac ttcagcgttc
    11881 cggcatcttc acggggtgtg aacaccggat tcgggttctt tacctgacgg tatgtgcttt
    11941 cagtgaatga ggtctggttc agggccgcca caagctgtcg cttatggcgg gaacagattg
    12001 ccgtgcgtta atgcactgcg aagggttgaa tctctgacac ggtttcatat atacttgtcc
    12061 cgtattccgt gataccccca ttatctttct ctgattccgc caagtttcga ggaaagatga
    12121 tgggggtttc gcctttatgg tcttctgaaa cttttttcag ccttccggca ccggcaaact
    12181 gccgtcgttt ctgccatacg tcctgaatgc cttatggcgc attctgccgt tttctggctt
    12241 tccggaccgc gaggatccca aaagccactg aataacgatc cgcaatatac cccttttcca
    12301 ccgctttgaa aaggcgacac gatttctcag agttacagtt gccctcttta accccctaaa
    12361 aagcgtcgtt tttttgaact ctgtcaaaaa aatgaacaat ttcacaaacg atcttttatg
    12421 ctgctttttt cttcttttcc agtagaagag atcgccgaat ttttttaaac gatctaatat
    12481 cccgcacaca acatcaaata tagaataata ttctgtctta tcaaatataa cgatctattc
    12541 aactgcttat cttcgctctt gccacagata catccttatg caatatattt tccagccatt
    12601 aatcctgttt acaggagttg tcggttttcg cccgcctcga aaccgtccac aaggcttaca
    12661 agagtgcgta aatcatgtgc ctgtcagaac tgcaggccag gctgaacgtg ggtcagccgc
    12721 tgtttccgac acatgcttta tgtattcgcc gttcacaaca acatcgaagc ccccctgcgg
    12781 ggatcgattt tcgcggagcg ttccggattt tgaatatcag tgaattacat caacgcaact
    12841 ggtatctggc acagtacatc cctgccggca aaaaccgcga acatctcttt tcctggcttt
    12901 ccgaacaaca cgtccttccc tggacacccc tgatactgaa aaaggtccgc cgcacggata
    12961 aagtctgtgg ttaccgtcga catatacacg ccgtgtttcc cggttacttt ttcctgaaag
    13021 cagaccccga aagccacagc ttcactcacc ttcgccgtca cagtgccttt ctggacttcg
    13081 tgaaaatggc cggtgaaatc aaaaccgtca gggaggatat cgttcagtct ttgatgaaag
    13141 tgtacccgga tcctgcgctc aatccggcag ccagggagga gctggatgct gcctccaccc
    13201 tctggctgac aaaagcgcgt taccagtacc tcctccggct tgatgcccag cctctgccgg
    13261 agtcgaggat cgccctgctc ctgcacctgg tatctgatga cggagcgttg acatgaaaat
    13321 tgctgtctta atttgacgac ttttcccggc ctttatggcc gggatttttt tcccttctgt
    13381 ttccacaatg cccggcaccg gtgacacctg agggtgtacg gagagcggct gtcgggcatt
    13441 ttttcaggac gcctttatga ccattctatc ccaggaaaac cctgtcattc tgttactgga
    13501 gaatatgctc gaagcactct ccaccgcccc ggacaacata aacaacgaac ggcgtcgccg
    13561 gcgttacctg ctgaactggc ttgacactgc ccgtcagatg cgcgagttca gaggaatggc
    13621 tgaagagttc actaccctgc gaaaactgct ggccaccaat tcagacatac cggtagcaaa
    13681 caccctgaaa tcactgctgg aaagtggcaa taacgcagca ttatacgacc tgtaccgctt
    13741 ccgctcggct cttgccacgt gcaggagtct gggatggcag attggcacgt gcgcatggaa
    13801 tgaccagtta ctgagtgaga cactttcccg cgcacagacc gggaaacgtc atatcctgca
    13861 gctgaacagc accggggaca ccttttccac cacgggcaga atgatcagac cggtggcatt
    13921 ttcattaatc catccggcca ccatcaacac gtctgaggtt gaagagatat tccgggatga
    13981 aggttttatg ctcgcacacg ggcgcgaatg cagtctgaac ggcagcggca gaaatatgct
    14041 gtcacgcatt cttcatgtgg gccattcagg tttagcggaa gaggaatggg gcatggacaa
    14101 cagtctgctt ttacactgaa ataccgggac agccacaacg gcagccactc tccgctgact
    14161 ttttttcgca aatcgcggtt tgctgatgtg gatgtcccat ttttgattat ccgtcctctc
    14221 cggttgtcat aaagcgcatt ccggcgtgag gcttctgttg ttatcatccc ctcctgcaga
    14281 catccccttg tcttctgatg actgacgata tgcttcttct gacaattaaa gaagcggaag
    14341 cttcacgcca taatgttgtt tgactgacct gccacaggag gaaaaatgac actgacgtta
    14401 cttaccgcgt ataacgcacc ctatctggca gccctgactt ttgttctgct tactggtatc
    14461 gctgaactta tcgccctgct ctgcggacac tccctctctt cagctatgga tacacctgat
    14521 ttaccggagg gtctgacagg agaggcgctg gactggctga acatcggacg gatccccctg
    14581 ctgatcgtcc tctgtatgct ggcaggtttt tttggtatat ccggcattct cctgcagggt
    14641 cttattattc atctgctgca ggctcctgct ccgaacattc tccttgcacc actttgcctg
    14701 ctgctgacat gtcctctcgt acaccgcacc ggcagactga ttgccccctg gttaccccgt
    14761 gatgaaacaa cggcgctcac cgaggaggca ttcatcggct gtatggccac catcactggt
    14821 tatcacgcag tggcgggttc cccctgtgaa ggtcgcttca cagaccgctt cgggcaggtt
    14881 cattacctgt tgctggagcc ggaggccggg aaagaattca gacgaggaga caaagtgatt
    14941 attgtctgcc gtctttccgg ttcccgctgg ctgggcgaac tgaatccctg gccggatgtt
    15001 ctttaatccc gacatataca gaggttctca ctatgtttaa tcttccggga tggttgttta
    15061 ccgttatagc gctggtcctc accctgttcg ttatcgggct gatatttgcc cgcctgtacc
    15121 gccgggcatc ggcagagcag gcctttgtcc gcacaggtct gggaggccaa aaagtggtaa
    15181 tgagtggcgg tgcaattgtt atgcccattt ttcacgaaac aattcccgtc aacatgaaca
    15241 ccctgaagct ggaagtcagt cgtgcagcgg ctgaaagtct cataacccgc gaccgcatgc
    15301 gtgttgatgt tgccgttgcc ttttttcttc gggtaaaacc atcagccgaa ggaatatcca
    15361 ctgcagccca gacgctggga caacgcacgc tcacacctga agatctgcgt agtctggtcg
    15421 aggataaatt tgttgatgcc ctacgggcca cggcggccag aatgagcatg caggatttac
    15481 aggatgcccg cgagaacttt gttcagggtg tgcagaacac cgttgcggaa gacttatcca
    15541 aaaacggtct ggagcttgaa agtgtgtcgc tgacaagttt caaccagact gcacgggtac
    15601 attttaatcc tgacaatgcc tttgatgctg aaggcctgac ccttctgacc caggaaacag
    15661 aacgtcgtcg tcgcgaacgc aacgaagttg agcaggatgt tgaagtggcc atcagggaaa
    15721 aaaaccggga tgcactctcc cgacgtctgg agattgaaca gcaggaagcc ttcatgactc
    15781 tggagcaaca acaacgggta aaaacccgta cggcagagca gagtgccagt attgctgcta
    15841 ttgaagctga acgacgtcgt gaagcagaat ccgcgcgaat tctggctgaa cgtaagatag
    15901 aggaagccga aatcgaacga cagcagatag tgcgtacccg ccaggtcgaa gccgaacggg
    15961 aagtggccat acgggaaatt gagcaacagc aggcaaccga aatcgccagc caggccagag
    16021 ccattgccgt ggcggcaaaa tcggaagagc agtcacaggc agaagcccgg gcaagtaaag
    16081 cactggctga ggccgtacag gcccagcagg atgtggaaac cacccgccag actgcggagg
    16141 ccgaccgtac caaacaggtt gccctgattg ctgctgctca ggaagctgaa acgaaagcgg
    16201 ttgaactgac ggttcgcgca caggctgaaa aagaagcagc cgaactccag gcagcagcaa
    16261 ttattgaact ggcggaggcc acccgtcaga aaggtctggc agaagcagaa gcacagcgcg
    16321 cactgaacga tgccattaac gtgctttcgg atgagcagac cagcctccgc ttccgtcttg
    16381 ccctgctgga tgcattacct gccgtcatag agaagtccac agaaccaatg aaatccattg
    16441 acggtattaa aatcattcag gtggatggtc tgaatggcgg tgttacccgc ggtaaaacag
    16501 caacaaccga cataagtggc ggaaatctcg ctgaacaggc agtaaccgcc gctctggctt
    16561 accgtacaca ggctccgctc atcgactccc tgctgaaaga ggtgggttta tccggtgaag
    16621 gacttacagc cctgacacgt ccactggtca tgcctgcccc ggaaaaggtt ccggatgtca
    16681 gcgaaccggt aaccgctccg gataactcag aaccacaaca ggtttaatcc ccgtgccgga
    16741 atttttccgg catctttttc acaaagaatg ccctcacggg atattctggt ttaaaagagg
    16801 attgaaaatg tccgcagaaa aattcagagt tgtcatatac accaatacat gtgaaaagaa
    16861 agtcagttat attcctgcaa cagaagatat aaacatcgct ataatgccat ttcagacggc
    16921 agacaatggc ttaattattg tgtgtcagaa ctcccgtata atacgacgca aagagcgtat
    16981 tcccggaagc agctgtgaat ggaaagatgt gacattagca ccaaaatcca gaaaccgcgt
    17041 tcagttatag cctgacagcc tgcttttcac cccccataaa agggggtaaa aattaaatat
    17101 ttcacaaccg cgatattttt caggaatgca gattattttc tgttatgacc aattgagaaa
    17161 caggcatgag aacaatcaca acacgtgaac aattattaat taatgggaga gtccacgaaa
    17221 gaatcgccac acatatagtt acaggagctc atgggtatga aacgctttgc acaaatggct
    17281 ataacgtcag atacaacaag gcgcagcaac tggttgaaag ctgcgaaaaa atagctgaag
    17341 gtaaactacc tgttacctgt cctgtctgtt tcaccatatg gcaggatgta cacagattca
    17401 cccacgttga ctttgacaca caatcgggga aaagtgattt tattgatacc tgccattcgg
    17461 agatcataac cggacatgtt tcaataacag gcaagaaata aaataatgtt ccagttctga
    17521 aaaacaatat cacttgtttt ctggaaacta tcatttgcag caggacgttt ttctttttat
    17581 atttccggaa ccggaatgtt ttagttttca taatcacccc gaacatgaat gttcatcatc
    17641 aatatcctga tgaaattata tttatctgaa gacctgttac cagccacctg tcttataccg
    17701 taacccgata cataccggat ttattctatg aaaaagaaat cacttacact ccttctgccg
    17761 tcattactca gcggctgtac ggcatttaat gccacgcctg ttcctgacac cacgcctccg
    17821 gctctggtgt tcgtcgacgg acaaattcgc gaaagcgctg ccattatcgc ccatacgcaa
    17881 aaacgtctgc ttccgccacc ggtgacccgc cctgtgacgc cgccccgccc gacaacaaca
    17941 gcgacgccca caaccgcagt cagcagccag agtaccgcac tgcctccccg tccgctcctt
    18001 caggccacac agctgcccgc actggttatg accggtaaac ccggtcagtc atccgtcgtt
    18061 tatttacgtg aggcacatca gcgctccgtc agtgactgga tacgccagtt gctaccaccc
    18121 ggctggcagt tccggcaaca gaacgctgcc ataccggcac tgaacaaacg cctccccgtc
    18181 tggtccgtta atgaccagtg gcaccgggca ctgaatgcgc tgctggccgg ccagcagctt
    18241 tacggacata tggactggaa caggaaaata ctgactgtca cgacctctgc aacaccgccg
    18301 gtcgacgtca cggcaccgca gggttcacag aaagctgctg atacaccgcg taatccgttc
    18361 aggggcagca ccgcaacgcc ggcaggcccg acaccagtga cacctgccgt cagcgtttcc
    18421 cgggctcaga ccaccaccgg gacatccgga actccagaga gcagaacaac cacgccagcc
    18481 ccggtggcca cgaaaccggt agtgccacca cagcagaagg tcataccgcc acggcagtgg
    18541 aaaatggaaa aggggaccac cctgaaagac ggactggccc gctggacatc atccgaaaaa
    18601 tgcccgcacg gcacatggac actgcgctgg gaaacacctg tcaactacag catcgatgcc
    18661 cctctggttt tcaccggcag tttccgccag gcgctggagc agctctttgc actctatacc
    18721 ccggctgaaa aaccgctgta tgcccggacc agcactgcac aatgcctgct ccgtgtgaca
    18781 gaccgcccgc aggaataacc tatgggatgg gttacgggtt tcattatggt cattatggcc
    18841 attggtaatt tctggttcca cagtcacctc agccgtactg tgcaccatca gcagaccgct
    18901 gaaatcacac agcaggctgc agactttatt cgttatatga atgccatcaa cgattacctg
    18961 tatcagcacc ctgaacgacg tgctgccggt ggtcagctca cgtcagcaca gctcggtctg
    19021 cccgccacga aaaacgtcag ccacctcatc agtcagcagc gcgtttttgt ctgggcaaaa
    19081 gaaaaacccg gccttatggg tgccctcctg gaacaaagtg gtgattcggc cctgcttgcc
    19141 agagtggaaa acggtcgcct cctggacacc cgtgggcggc gtatttccat cacgctccct
    19201 gctgtgatcc ccgatcaggt catcatctgg atgaattagg ccggactctg aataactcac
    19261 ggatttatct atgaacaaaa aacttcttgc tattatgatt gcagccactc tgctgcaggg
    19321 ctgtaccagt ttccgacagg cagagaacac cagaaaagac gccgttgctg aacaacaacg
    19381 ggcccgggaa atgatcgctg agctgcgaaa ccggacaccg gttgtaaagg aatccccacg
    19441 tcagtggatc aatccccgtc cggtcacgga taaaaacccc ggtaatgcgg tgcccggctg
    19501 tccgattatc attaacaccc ggcagagcat cacccttcag caggtggccc agcggatctc
    19561 gcaaaactgt catattcctg taaggatcac ccctgatgta caggcgtatc tgagcgtgac
    19621 cacctccggc agtacgcagc aaatccaggg cacagtacca ccgccggata ccggcggtat
    19681 ggttccactg gccgccatag gcagttctga cacgacactt ccccgcctgc agggaaatac
    19741 ggcgtcactt cctgaactga aggcttcagg actgacatca ctgctgaatg cggtcgcttc
    19801 ccgtctcggg atttcctggc gttatgatgg ttcacatatc acgttctatt atcttgatac
    19861 ccggaccttc ccggtgacct atatggacag ccaggttgcc tataatgcca ccgtggtcag
    19921 cggtaccatg tcatcaaatg gctcatcagg tggaacagca tcggatgcca tttccggtga
    19981 tgccagtaat acccagacca ccaccgtgga tatgaaatct gcgctgtatc aggatgtgaa
    20041 aaatgctgtc tcatcaatgc tgacgccggg tattggtcgc atgttcatgt cgacgggctt
    20101 tatcaccgtc acggacaccc cacaggtact ggaaacggtc cggacgttca ttgaaaaacg
    20161 caatgaagaa atgaagcgcc aggtggtgct gaatgtggaa attctgagta tccggaaaac
    20221 ccgtaatgag caggcaggta tcgactggaa cgcagtcttc agcgacagaa cgctgggact
    20281 gagcctcggc agcacattca ccagtgcagc cagcgatacc gtcactggtg gtgtttccat
    20341 tgtgaacggg aaactcaccg gttcaaaagc cttcctgaaa gcactctctt cccagggaga
    20401 tgtcagtgtg gtaacccgga attccgccgt gaccaaaaac ctgaccccgg taccgatgca
    20461 gattgccaat cagcagagct acattgagag tgtgacgacg gataccaccg ccaatgtcgg
    20521 ctcatccacc tccctgaatg cggccaccat caccaccggt tttaatatga cccttctgcc
    20581 gttcattctc ccggactcac agacactgca gctgctctat tccatgagtc tctccgacaa
    20641 accggttatt gagaattacg agagtggtgg aagcaaagcc cagctgccga atgtggatct
    20701 gaaaaccatc aaccagacag tggacctgaa atccgggcag acagtcatta tcagtggctt
    20761 ccagcagtca gggcgtcgca gtggaaaaca gggtgtcggc acaccgggat tcttcggtct
    20821 tggcggtggg atcaacagtg aaaacgacga caccattctc gtggtgctga tcacgcccaa
    20881 catcatctga tttgcagaag gaggactatc gtgacagata tttcaccgac agagcgacag
    20941 gtgtttccgc tgacggcagg acggaatgta tttcttgccg gaatggactg gaaaacactg
    21001 cccgcaagcc ataaaaaccc ccggacgttt gcccgcagtc tgggggcagt acgctatatt
    21061 tcctgtgagt atctcagtac ggaagatacc gatcgtcaca tcatggttgc agccgtcagc
    21121 cagaatacac tccccaaagg cagccgcagg tatttctctc tcgccatgct tatcctgcct
    21181 ctgctcgagc caggaggata cgctatcgtt gaactgtcac aggcaaacga tacagaactt
    21241 tatggttttg tttctgctgt tgacggcatc ctggtcagtg acctggtggg aacccgggag
    21301 gaaatcaggg aggcccggga aatattcctc accattaaca gcgcgcctga acacggctgg
    21361 acatgttatg aaccgccctc gtttaacggg cctgacggca gaggtcccct gccactggaa
    21421 accctgacag gtgcagggaa atatccggca gaagcacgcc ttcaccccgt atcccgtggg
    21481 ccacagctta ttccggtatt actcatactc acccttctgg gtactgcatg gtatggctgg
    21541 cagtactatg aacgcctgaa ggcagaaaaa gcagcacttg ctgaagcggc acaaaaagcc
    21601 gcagaggaac ggatcacacc accgtggctg agtatgccgg aaaccggaga ttttatccgg
    21661 ggatgcagtg acgtatggtc acgcctgccg gtgtcggtcg ccggctggcg atattcactc
    21721 gccgagtgtg ctgccaccgg gaacagcggc aatctgcgtg catcatatac aaacatcgcc
    21781 gggacgacag tgaccgcatt cagccagcgg gtcagggaaa ttttcagcat ccggccgttt
    21841 atggttatgc cggacgggaa tagcggtggt tttgccctgc cggtcacctt cagtatgcct
    21901 gaaactcccg ttgccgttga ggcattaccg gaaaacaccc tcctgcagga acggctgacc
    21961 acactcgccc ggtcgatgca gctgaaaatg gactggcagg aagtcagcaa cagcttcacc
    22021 gatgaagacg gaaataccat ccagcccccc tggaaggagt atgacctgca gatactgacg
    22081 acacttccgg cgcaccaggt ggcagagcat ttcagtgaac cctccgtccg ttttatctcc
    22141 gtaacaaggc aactggaaga gggtcgtttc cgttatcaat tcacaggtaa atattatgtc
    22201 cagtaaaaca aaagctcttc tgctttccgt cctgatcctg ccgacaacgg tcgcggccag
    22261 caccatcagc aacatgcaac agaataaccg gcaactccag gtggaacaga ttcaggaaga
    22321 aactgcactg tatgaagcca tgctggcaag agaaaaggtg cgacatgaac tcagaaccct
    22381 ggagcaggga gaaccagctg agcctgtgca ttcacaggaa agcagttcag ccgtcacgac
    22441 agatacccct gcccccatta ccggtattcc ccggctgaca gagatatccg gagttcaccc
    22501 ccagataacc gccaggatac aactgccggg cggacaaact gctgatgtaa aagaagggca
    22561 acacattccc ggcaccacat tccgcgtgga aaagatcacc actgacagtg ttgtccttga
    22621 acaaaatggt atcagaacaa cactacaacc ctgactcagt gccgccattt acagccggct
    22681 gtcggaacag attattcccc cggcccttaa tcacaaaaaa aatcgtttcc gggtaccgct
    22741 gcccgtaagg agattttatg ctggcacctg cagaccagcc acctgatgac attctggatt
    22801 acgtggttgt taatacagat aaaacaggac aaattcatgt cagagtggtg gaaggaaaaa
    22861 aacaccttcg tgccgtccag gattatctga cccgcctgtg ctcccgctat ccgggtaaag
    22921 tatccccgtt tgagttcacc acacttgatg tgatcgcccg actcaggcag gagacggtca
    22981 tttccggggt ggaaaacgga attaaccccg tacagcaaaa aatgctcggg tatatcagcc
    23041 atgcggccac cctcagcgcc tcagatcttc acatcacgcc ggggcgtgac agtacggatt
    23101 tcacgtatct ggaggcccgt gtccacggag agctggagct gctcgatatc gtcagaaaag
    23161 atgaggggct ggaattactg ggggccacct attccggaat gactgacgtc ataaagggaa
    23221 cacagtttga tcccggtgtc ccccaggatg cccgtctggc tgaaagattc ctgaaactgg
    23281 ccggactgtt tggtgcgcgc tactcccact atccgtgtgt gggcggactc tatgccgtac
    23341 tgcgtctgat caaggatgac agtcagcata taccgacatt cagtatgctg gggtatcacc
    23401 ctgagcagga gcgcgctgtc cgaaggatgc tgcagcgacc ggagggcatc gtgatcctct
    23461 ctggccccac cggttccgga aaatccacca cgctgaggac ggcttctgcc gcctatctgg
    23521 aacagtatgg cttcaacgat accggaggga tcctgctccc ccggcgacgt ctgttcacaa
    23581 tagaatcacc accagagggg cgtatccccg gtgcaattca gacggccgtt atggataccg
    23641 ctcagggctg ggtcgattct gttaagtccg ccctgcgcct tgatcctgac gctatcctga
    23701 acggggagat acgtgaccat gcctccgcca tcacggcgat aaaagccgcc atgaccggtc
    23761 acctgatgct gaccaccctt catgctaacg acccgataaa tattcttgag cgtctggaaa
    23821 tggagggcgt tcaggcaagg atgattgcag atccgcagtt atttatcggc ctgctcagcc
    23881 agcgtctggt tcaggtgata tgtccccact gccgcctccc ctggcatgag gtggagagct
    23941 cccggacgga tgaagaacga cgcctggtgg aaaacttctg ccagcccgat gccgtgtatc
    24001 ttcgtaacca taacggctgc ccacactgct ggcgtggagt gaacggccgg accgtcattg
    24061 cagaagtgat ttccccggat gcgaagtttt tccagattta ccgggaaaaa ggccggattg
    24121 aggcaaaaac ctactggcac cgggagctcg gcggtatgac acgaaatcag cacctgctgg
    24181 gcaaaatcaa cagtggtcag gtcgatccgc tggctgcgca ttacatatcc cctgtagacg
    24241 aggacagcta tacgctgctg cactgatatg aaaattctgg aaagactcaa caatacggaa
    24301 ctggaactgt ccggccttaa ccgctggctg ggcaaaaaga cgttcaggcg gactacgttt
    24361 tacgagtccc ttgcaggcat gatccgggac ggcacaccgg ttatgcgggc actggagttc
    24421 atctgcgatg tggaaacgga ttttggtaaa aagaaagggc agtccggcct gtattttctg
    24481 gcgacagact gtatcgccag catacgcagc agtggtcagc tctctccggc cctgaaggac
    24541 tgggtaccca aagatgaaat tgccctgatc cgtaacggtg aagaacgagg tgatatcgct
    24601 gaagccatgt ttcaggtcgt caaaacggca aaaggacgac aggagatgat tagcagtctg
    24661 gtgagtgtct gcctctatcc gctcatcctg ctgacactct gtgtggtgaa tatgtataac
    24721 gttcataccc gggttattcc gatgatcagc gcctttgcac cggaagaacg ctggtcagta
    24781 cagatggtca ttctggccaa atcctccgga ctggttatcg gttatggttt gtggctgctg
    24841 gcaggactgc tgctgctggc aattctcatc aggatgtcat tcagtctgta taccggtccc
    24901 cgacgacggt atcttgatcg catcccgcca tggtccctgt atcagacttt ccatggcgtg
    24961 aattaccttt ttaatatttc atccatgctg cagataaata ttcctttatc tcatgccctt
    25021 accagaatag aaaaacatgc agaacataac cgatggttaa aatcacgaat aagtgctatc
    25081 agaaaaaatg tcttatccgg tcagtcactg gcactggcca tgaaaaactg tggttatgac
    25141 tttccgtcaa aatcctgtgt gaataatctt cttttaaatt cagaaaggga ggacagtgtt
    25201 gccagtatgg cgctttatgc agaccgctgg ctggaagaag caaaaaagaa tgtcagaaaa
    25261 agcggcatta ttattactgc tatatcaggt ctgctggtat ttctcttcat catcaatatg
    25321 gtcagtgcca tttacggcat cagtgacatg ctgaagcagt aaacacagca tcctttatat
    25381 tcccctcgat ccaaattctg ttttaccaat ctcaggagct aatatgagcg tcataacctc
    25441 ttcgtattca gaacataata ttcgtcacct tcagaacgat gataaaggca tgaccctgct
    25501 ggaagttctc ggtgttctgg tcgtcgctgc cattgttatt ggtgccgtga tgggactgat
    25561 gtccgatacc ctgtcctctt cagacaacca gaaggaactc aaaaacctgc agacaatcgc
    25621 caccaaaatg aaagcccaga aattccaggg gcagtataca ggtactgact acgtcaaaat
    25681 cctgaccgaa agtggtggcc tgcctgccga tatgatcgcc ggtggtaata aggcgaaaaa
    25741 tgcctggggt ggtgcggtaa ccatcaaagt cagttcagat aaatactcct acgttattga
    25801 atccagcaat gtaccgaaga aaaactgtat tgatctggtc accagcctgc gcagttcatc
    25861 catgtttaca aaaatcaacg gcaatgtgac gaataaagtt gacccgtcta cagtatgtaa
    25921 cgcagataaa actactatca aactggaaac caacagttaa tcattcatca gcgcccgccc
    25981 gggcgcttta tttaaggtca ctgtatgaac aggttattta tcctgacagt gattttttca
    26041 ctgttatgct cacttccttc cgcacaggca tccccacagg acaaacaata tgatcgttgt
    26101 tttattaccg ccggaaaacg ttattccatt gatccaggat tactcaaaat tattgcccgc
    26161 agggaaagca gtctgaatcc ccgggccatt aatcataaca ggaatgacag aggaaaaatt
    26221 atcagtaccg attacgggat aatgcagatt aactccacac atattcccgg gctgaaaaaa
    26281 atgggcatta tccggagtga gaaagatctg ttgaataatg tctgcctgaa tattcagacc
    26341 ggagcatgga tcctcgcccg gcattttcag cgatgcggcg taaactggga atgcctcggc
    26401 agttataacg ccggcttcag taaaagtaac actcaccgcc ggatgaaata cgcccggcag
    26461 atttacagcg cttacatgca gggacgttag tccgggagaa ctgccatgct ccagataatc
    26521 ccggcactgc tggtcgccat aaccgtggta atacagtgtc tggccgttca gatgagtcgc
    26581 tatcgactct cccggatgaa tgcccgcccc cctcaccatg ggctggcgct gatgagcggg
    26641 atgcttgcag ctgccgccat tatcctgcgt ctttatcaga atgacagcgc tgtcggggtg
    26701 ctgtttatga cgataatgac cggatgtatg acactgatga ccctgacgga tgctgcatcc
    26761 tgccgtcttc cccggccctt caccatcatg tatctgataa gcggcagcgc atttcgtctc
    26821 tggcagggag actggctgac aggcctgtgc agtgcagggt tctggttcct gctgttgctt
    26881 ctgttcaggc aatacacctc ttaccgcagg aaaacagaaa ccataggcct gggcgatgtt
    26941 tttctgattg ccggtattgc catctggtcc ccgccgggag acatcccctg gattgtcgca
    27001 acggcagcag gcggagcctt actttatctg tggtgtaaca gacaacgacg cattaccaga
    27061 gaacttccct ttgggccgtt tctctgcgcg tcacagtacg catttacttt ttatcccggt
    27121 ggcctttggt aataccggag ggaacgacta tgcaaaaaga caacgataaa ggttttgcac
    27181 ttcttgaaat tctgggagga ctggttgtca tcagcctgct catgccgctg ttctggtctt
    27241 acattgaaga ctacctgaat gaaatgcgca accagagcgc cgcatttcat gcagatgcct
    27301 acaatactgc agccaggacc tacatcgccg ataacaatgc ccgcctgcat agcggaacgc
    27361 ttcctgccac cttcacggcg gatgagttaa tccggaaagg atatctgaag ggtcttaacc
    27421 gctcgccctt tgggcagagt tataccaccg gcatccgtcg taacacgagt accggtcgtc
    27481 tggaggccct gacctgctcc accggagggg agaatatcaa agacgatgcc ctgcgcagta
    27541 tcgccagcct gctccccggt ctgggcggat ttataggaaa aaatggcaca gcaaccggtg
    27601 tttttggtgg atggacagat aaacccggcg actatggact cagctgcaat ggcgggcaca
    27661 tcgccatcgt aatgatgggt gatgacctac aggaaagcga tcgcctgtat cgctttcagg
    27721 taccgggacg accggagctg aaccagatga acacggccat caatatggga gggaacaacc
    27781 tgaacaatgc aggaaacgtc aatggacaaa gtgcaacact gaaaggtgat gttacaagcg
    27841 aaaacggctg gttaatcacc aaaaatgata aaggctggaa gaacatcacc tatggtggcg
    27901 gttttacgat gacagacagc cagtggatac gcgcagtcgg tggtaaagga attataacca
    27961 caggggaaat caaaggagga aaagtcagtg gggggacagt ccggtctgac ggtcgccttt
    28021 ccacaggtga gtatttacag ctggataaaa cagccgtagc aaatacaaag tgttcaccag
    28081 atggacttgt aggccgcgac agcaaagggg caatactttc gtgccaatcc ggtgtgtggg
    28141 ttggctttga atcctaccct gtgggaagcc ctatcccgtg gccatctgcc actcctccac
    28201 aaggctatct gttgatgaac ggccagtcgt tttcctgttc ccggtatcct cagcttgccc
    28261 gtgcttatcc tagctgtaag ttacccgatc tgcgcggtgt ttttatccgt ggctgggata
    28321 acgggaaggg acttgatggt gatcggaaca gacagctcct gagttatcag gccgatcaat
    28381 caggcgtcta tcacgaacgt ggcggatggc tgaaagggca tcatagtggt atgccatact
    28441 gggcacaggg gagtaccaca gaaatgcgtc ccaaaaacat tgcattcaac tacattgtta
    28501 aagccagcta gggactcaca tctgataggc atacacagag aaaacaccgt taccacaggc
    28561 gtaattttgt gttggtctgg agatgatttg atatgacgct cccactggca cggcaaatga
    28621 aataaatctc cgggcatatc agctggtcag caaactcgat gccaggcgta aacagaccag
    28681 caaaatttcc ccttattttg ttccctaccc tgcaactgtc gggtgcagaa acgggctgtt
    28741 tgtcgtgaca ctgcacgatt tcgaccttga aacccgtgca gaaacccggg gactggcaat
    28801 atctcatgcc agcatgctca gtcacctcct gtccgaatcc ttttcattcg cccttaaaac
    28861 agagttgcaa acactcttga aacacactcg atcataacga tcgaaataac tcgcttccgt
    28921 ttaaaaaaca ctctctttag actcaattta tgaacaaaac acgctgaccc gcatggaggt
    28981 gaaatgaaaa ctgacagcac aggtattgcc gcccgtatga tgctctccct ggacagggag
    29041 cgaatttgcg aatgcctgct cagtcatcgc cagctgcagt ccacccccct gcaggttcgt
    29101 tatccgcagg gagtcaggga tgcccttggt atcatgagcg aacagctttc tctttccgtc
    29161 tctgacctga cgcgtatcct ggtcgaagac gcgttaagtg aaatgtttct tcctgcagat
    29221 aacatcgtcc gccgtcttct cagcaggatg gagcacatca tgcaggcaca tgatatcagc
    29281 gcaacaacga tggctgcatt actggccccc tggaatatcc gccctgccgt cttccgggag
    29341 ccggacagac tcactgacta tctgaccggg gagattcttg cagcactggc tgactggttt
    29401 tatctcagtc cggaatggct gaacggcagg gtgcattatc cactgtatcg tccgggagac
    29461 tggccagcca cacaggagat attctgcagg attatttcag cccgcgagaa tatggacatt
    29521 attctctggc atggttttcc gtttgccgga acgcattccg gagaatattg cggcgttctg
    29581 ctcaggcaga aaaaagaaat taataacacc attatttatc cggtactttc gttatatccg
    29641 gccagaatgg atatagaaaa agaaggctgg tttcagatgg cccgaaaaat atctccggat
    29701 atccctgttc gcgccgtaac actcactccg gcacaggcag aatatctgat aacaggaaaa
    29761 atattaccca ctgcgttatt cagggttccg ttatccccct ggtaataata ctgagcaata
    29821 aaccggaata atacaccaac tgtttttaat ggcacagccg tgccctctgt tgttataccc
    29881 aaaatacctc aatacaggaa actgaaatga tgatgaaaaa gcatattacc cgtactctca
    29941 tcgcgtctgc cgttttattt tcctttaatt cgactgcagc aacgtcatat tttgaagccc
    30001 gcaatgatgc gatgggagga acgggggttg cctcctctca ctatggtgtg gcaccactgg
    30061 caaacccggc actgctgaca aaatccggtg caaaagatga cttcagtctt ctgcttccgt
    30121 ccgtgggtgc gcagctgtcc gatccgggta atatcgccga caacgccgac aaaatcagcg
    30181 atgactggaa agccttcgac cgtgccgtcg acagccactc cggggtaccg cagacagcgg
    30241 cccgcctgaa agagagactg caggacttcc ggaacaccca tgccagcgca caggccgggg
    30301 tatctgctgt agcggccctt cccggtgata ccctcgctgc tgcactgatg ctgaaaacgc
    30361 tcgggactgt cagcgtcgac ggtaaagtca gcgatgccga cctgaactat ctggaaagca
    30421 ttgcagacag tggcagccag gatgtggata aaaacagact gacctcacag gcttttgccc
    30481 gtgccgcact gatcaccgat gtgggcgttg cgctggccac tgaactggaa acggccggac
    30541 aaaaatggtc actgggcttc acaccaaaat tccagcgtgt tgacctgttt aactacaata
    30601 cgctgatcaa aaactatgac agcagcgcat tcaaaggtga ccgttaccac aacacccaaa
    30661 acggtattaa cgctgatatc ggtgccagca tggacctgga tgacaactgg acgctgggtc
    30721 tggtcgcaca gaacctgatc ccacgcagca ttgagaccaa agaggtacat ggtgtcactg
    30781 aaaccttcag gatccgtccg caggtaacaa ccggcgtatc atggcacaac gacatgttca
    30841 ccacggcact ggatgtggat ctgaccccgg ccagcggctt cacatcagac agcaaacgtc
    30901 agtttgccgc cgtaggtgct gagtttaatg cctggaaatg ggcacagtta cgtaccggtt
    30961 accgccagaa tctggccagc aacaacggca gtgcattcac tgccgggatt gggctctcac
    31021 cgtttgatgt gattcacatt gatgtcgccg gcctcgtggg caccgacaac aactacggtg
    31081 ccattgctca gttacagttc accttctgat tatcacgccc cgggacgccc ggggcttctg
    31141 acctgaatct gctcgtttcc gctcttctgg ccactcaccg ccagtcctgt tcattatttt
    31201 ccggataaaa ccatgcaaaa aaaacgtatc acccttgcag ggggagttgc catgctctgt
    31261 ctgagcggct gtcatcttcc cccggataat accgcaccgg ccaggaagta cggtgcagct
    31321 tctgaagtcg ctgtcacacg acatgcacag tatcagttgt gggagcaggg cacgtattct
    31381 gcagccctgc cttcccgcca gcccggaggc agcctgcagg ctcacagcgg gcgggtttcc
    31441 tttgactggg aaggcgatgc tctggagctc ctgagcgagc tggcccgtct gcgcggacaa
    31501 cagttcagtt acagcggcgt gcgtcttccc ctgcccgtct cactccatgt acgcgatatg
    31561 accttcgctg atgcccttcg tctcacagag gcccagactg cctggcgggc agtcctctca
    31621 cagtcagccg ggcgtttaca tctttcctat atgccaacgg agaaaaaatg aaaaaacagg
    31681 tctgtctcct gctgtccggg ctcctgctgg caggctgtgc cggacagcac catcgccccc
    31741 ctgccggtat ggcaggcaat ttaccaccgc cggatctccg ggcatggctg acgcccactg
    31801 acaaccagga tgacggcatt tcagacaccc gtcgccagat gctcacggac gccggacgaa
    31861 cgctgggatt ccgtggcgga aaagcacagc gctcatggga actgattcag gcactgaatg
    31921 cccgggaaag tacactgaat gccctgtatg acttccgtcc ccttatcagc agggaaggct
    31981 ggctaccccc tgttattgat gaagcgcagg atgttgctca cattcagcct gaccagataa
    32041 gaaccgcgtc ccgtgtctgg accatcctgc gccccgaacg ttttgtcagt aacccgccgg
    32101 gctggcgtga ctggcttctg cgggggcttt ccactaccgc caccccggga actgagggcc
    32161 gtgttgtacc ggaagaccgc gcacagcgca gactctggga aaacgcactc cgccagggct
    32221 ggcaggaagg ccgtgacaac gctgacctca ccctggaagc caaccagaaa cgcctgaccc
    32281 gggattaccg gggaatgatg ctttatgccc tgctctggcg tcagggcatg atcacacgcc
    32341 cggatgtgac tgaacagcgg cagaccgtca ccggtaacgg caggaaactc atcaccggag
    32401 accacgtccg ccgtctgaag acacacgcgg aattcaccct gcagaaatca cgctggcgtc
    32461 cggtcgtcag cacggaagga gcccccgatg aaattacccg atgagttcgg cacattccct
    32521 ttcgcccgtt tcacggccga agagtttcgc tatttttttg cctggtgtgc ccgacaccgc
    32581 gtcagtgata tcgatctgac aggcggctca ccggtatctg tcaatcgctt tggacggcgg
    32641 gtacgttcgt ccgcacatcc tctgccgtcc tccatcatga tggcgctgag tgatgaattg
    32701 tttggacggg aagtccttcc ccgggtgctg tccggtcatc ccgtggaccg taccattcag
    32761 gtcagcggtg atgcctccgg tcgctacggt ctgcaacggg ggcagcgtgt tcgcctgcgc
    32821 tgtcatctga ttcagggaac atcagcaaca gaagaaaaag taatctccgt caccatgcgg
    32881 gtgatcccgt ccgatattcc ggacatcctc accatgaata ttgaaccgga cctgctggat
    32941 gccatgctcc ggaaaaccgg tctgggtttc atctgcggag agaccggctc cggtaaatcc
    33001 acactggcag cggcactgta ccgctacatt cagacgcatt tccccgaccg caaaacggtg
    33061 acctatgaag atccggtgga atacattctg gggggagaga atgaccttct gccaccccat
    33121 caggccgaaa tcgggcgtga tgtggaaagt tttgcggcag gacttcgctc tgccgttcgc
    33181 cgtaacccgg acatcatcgg tatcggtgaa atcagggatt atgaaacggc agatgcagcc
    33241 gtccaggcag gtaacaccgg tcacttctgc atcggcacca tgcacaccaa atcacccggt
    33301 gaaacctttg cccggctgct ggggttattt ccaccggaaa tcagggattc aatggcagcc
    33361 gccacccttt ccctggtgca gttcattctg gtgcaggtcc tggtcagaac aaatgacggt
    33421 ggcagacagg ccgtccggga atacatcgtc ataactgacg aactgcgtga tacgctctcc
    33481 cggcagtccc atgcgacatg ggggcattac attgatgaaa tcattcgcaa ggaaaaacgg
    33541 cggatacggg atcaggttct gaccatgtat cagacgggca gcattgaggc atcagaagct
    33601 gcgctcttca tcccggccgg agaattcccc aaatgaaacg cgaaggcttc tggcgggatg
    33661 tcactccgcc actgaatatt tatggtgtcc cgttgccgtt taccatgatt tacctgctgc
    33721 tgtttccgta tccgtcaaaa acaacattct gggtgtgcag cggcattttg ctgttttttg
    33781 tgctcctggg gtactcagga tggactgtcc ggacgctctg ccggcgtctg tacagcctgg
    33841 tgcagggtaa acaggcgtca ggccgtccct ggtggtaccg gcatcgcaca gaattacccc
    33901 gtgaatggcg cggaacggaa taaccctctt ttgttcatca tcccggcgta tcacaacgcc
    33961 ctcatcttta atccccggag aacgacatga agtccagata tcgaacctgg cttgctgtcc
    34021 cgcctgagga gacagaagcc gtaaaaaatg cagttccccc gttaaatggc cgcaaagctg
    34081 tcgcatggga tccagaaaaa aagctctggt atgcccgggc gggtaccgaa ctttcactgc
    34141 tggagcgctg gttaccccgg ccacaggaac tgtcgatgga tgccggcgat ccggtcactg
    34201 aatttgctca ggtcctggaa aacgccgggc tggttattca gggattgcca cagatggacg
    34261 gagccataca cagagtggcc acccgggatg acaaaaaagg ggccaaaagt ggcgcataca
    34321 gggcctatct tgatggtcgt cccgccggct ggtaccgcga ctaccggagc gccgatgaca
    34381 gtcccacaaa ctgggtattt tccggcggag aacaacacga tcctctggcc agactgcatc
    34441 tgcgggcatt cgctcagcaa cagcgcgatg ataacgctcg taaactgcag caacagtata
    34501 acaaacaggc cggctatgcc cgctcctata ttaacaggtt gccacaggca acagcacatg
    34561 aatacctgac gcgcaagggc atccgggccg cgcctggcgt gcggctgaac aataaaaatg
    34621 agctggttat tcccttcagc aacggccggg gagaaatacg ttcgtaccag cgtattccgg
    34681 tcaccggcgg gaaggatgcc cgcattctga aagactccga gaaaaccggc aactggttca
    34741 ccttcggaac cccggaaaat ggccgccccc tgttatttgc ggaaggttac gctactgccg
    34801 cctccctgca cgaagcaacc ggccttccgg ttctgatgac agttgatgcc agcaatatga
    34861 ttgccgtggc agaaaatgcc cgccaaatct ggactgacag cccctttgtc ttctgtgccg
    34921 acaacgatca tcaacgggaa attaacaaag gggtttttag tgcaacaaag gccgcagaag
    34981 tcacgaatgg cgaagtcatt attccggcct ttacggaggc ggaaaaagca cagggactga
    35041 ccgatttcaa tgacctggac gccagccggg gacgggacaa ctttcagaat gccatgaatg
    35101 cacagttaaa acatatcggt atccccacac ccaacagtga cactgccgac catcgtgagg
    35161 ctgtcgtgat cgacaacctg atattcacac cggtaaaaga tgagaagcca caaatgtccc
    35221 ctgaaaacag acagagcaca gccccggaaa cggagctggc gacacaggat acgccttatg
    35281 acacccactc cgacgataca gaaaaccagg catatgaaga tgactatgcc gcctatgcat
    35341 ccctgcaatc agtaactgcg gagacgttat ccccggataa accactggag aatattgcgt
    35401 ctgccgctga taccggcaca gagaatacct ctgtgcctac cgctggcgac caggaagacc
    35461 tcaatacaga cgccacaggt acagacaccg ctcacaacag cgacacagca gaaacaggaa
    35521 cgcttcctga acctgccggg atgcagtccg tgcagcaggc aaccactgaa ccgaccgcag
    35581 aagataaacc acacgagaac gttgtttccg ctgccgatac cagagaagaa aatgcctctg
    35641 tggctaccgc tgataccggt gaagaaaatg cctctgccac caccgctgac aaccagagca
    35701 cgcaggcaga agaacatccc cggagtgaag acacaccgga aacctccctc tccgacgcgg
    35761 ccgggacaca gcacaccacc acaacgcagt ctgaagaaca agtgcaggtt tccatggcca
    35821 gcgctgacga ctggagcgca tttgcggaag atctcaacgc tgacactccg ggggccccca
    35881 ctgttcacaa caccgacaac gcggagacaa acacagtttc tgaaccggcc gggatgcggc
    35941 cagttcagca ggtgaccact gaactgacgg cagaagataa agcacaggaa aagagcgttg
    36001 cgtctgccgc tgagaccggc gcagagcact cctctgacat caccgatgac aacaggaacg
    36061 cacccgctga ggagctcaat gccgatgcca cggttgctgt tcacaacacc ggcacagcag
    36121 aaacagcctc accttctgaa cctgcagaaa ctcagccagt ccggcaggcc accgctgaac
    36181 cgacggctga agataaactg caggaagagc gtcttgtgtc cgccactgag accagcacag
    36241 cgaatgaagc agcccgccct gaaacgccat cgcttttcag ccgtctgcgc cgttttctga
    36301 ccggtgtgaa atcacgcacc tcagaacctt ccgttattca ggagaaaaag cccacggagg
    36361 ctgaaacagc aacagaggag gtcgcaccac catcaccacc ggaaaccagc ccggaagaca
    36421 catcgtcagc ggaaccggac acgattatct ttgcaccccg ccgtcccgac agtccgcagc
    36481 ggcacaacct ggacgagatc cttaaaaatc ttacacacaa agagtttccc gacaggactg
    36541 cgctctacag cgttgacggt gaacccgcct tcctggacag actctactgt ctcgaaatgg
    36601 tggatggcgc cagcgcggat gacaaaaagg tcctggctgc ccttgctgtg gccaccaact
    36661 tctatggcgg cgttatcgag ctgaccggca gtgatgcctt taagcaaaaa gccatgcagc
    36721 tgattgtcga atacgacatc aaggttcgca tgaaactccc ggcccagcgg gcagcactgg
    36781 aaaaactccg caaggaaaaa gagatggcgc atgatgccat tgtcacccac agacccacgc
    36841 cggaactcaa ccgccagtca ccggaagaga caacaatggc ccaaccggct ccccctgtgt
    36901 caccgaaggc agaggctgaa caaacagcag gcagtacggc tgaaacaccg catccggaag
    36961 cgccggtcac ggcaaaagct gaagcccccc gggccgacag accggaacac cccgccgccc
    37021 cgtcgtctgc gccagaaaaa gcccctgtca gtgcacagcc gcagcaaccc gacacaccgg
    37081 tggaagagcc gcccggcaag ctcagccccg gtgaaagtgt gactgccgtc ctgacaaact
    37141 acggagaaaa agagtatgcg cccggaaaag ggcgctgctt ttttgtggag ctcaccaacc
    37201 gcagtggcaa acgggaatac tggggaaacg gcctggaaga actggtcaaa aaccatcaga
    37261 agggtgaccc cgtcacactg acactgcagg cgagagaaac attccggctg tccggtgaaa
    37321 cgcgggatcg tatccgtaat atctgggcca taaccccggt cagtaacggc attaccgtta
    37381 ataatgacca acctcatgcg ggtcagcata ccctggagtt tccgatggca acattcagcc
    37441 aggtgatgtt acagatgcaa caacactggc cacagataat gcaggaagtt cgctccccca
    37501 aaaatcctcc caataatctg tacattggtg aagacaggca acctgcatca gcacctcagg
    37561 atcaatcaat gcgactcccc ctcgctggta atgcccccaa tgaactgata ccagccatcg
    37621 cctctgccga taaggacaat cgtcagttga atcttctgct ggtccacagc gcagatgatc
    37681 atctgcaggg cgtggtccgc ctgaacggca ccctttaccc ggcactggcc accccgtcag
    37741 ctgacaaccg tcagctggtt atcaatgccc tgacggataa cggtctgcag tttgccggtt
    37801 acggtgaagc tgttaaccat gatgagaata atcatcagcg tccgtcccca caaataatgc
    37861 agtttcacct gaagcaacag gactccccgt tgtttgccgc catccacaaa ccggaagagc
    37921 aaccggacaa gcttttccgc tctctgggtt ttgagcagac ctggaaagag tggtctgaca
    37981 gccaaaaggc ggaggacagg caggaaaaaa ctctgcaaca ggcacaatcc cacagccccg
    38041 gccgatgagc cggtaaggac gaccatgaac atgaaaaaaa cgctgctggt catactgacc
    38101 ggcatcaccc tggcatcacc ggtggcaggt gccgccacct gccgggcggg ggccgctgcc
    38161 gaagccggca gcagagccgc ctgggagcgg gcgcgcacag ccagccaggc atggtcacag
    38221 cgggagaatg acgtcgcctc ctccctgcag tcctgcctgt cccgcatcaa ggacatcagt
    38281 atcactcttc cgcagttccc cagcctggac aagctcctcc gcgacctgga aaaccaagtc
    38341 tgtgatgccg tcgtggatcg ggtgaatgac catcttccgg ggaatatcga cccctggaaa
    38401 gactacaacc tctgaacagg agcaccatga gcgaagcaca acccacctcc cctgtctcac
    38461 cggaagcgga taacagcgga gatatttata accccgtgct cagaaccatc gcacagtcag
    38521 aacaacgggc cgcgctggta cccggtctgg tcagggcgtt gctgtacagc ggcgcagcac
    38581 tctgcatcag cctggtggct aatggcatac agtactggca tgccaccggc gtgacgcgcg
    38641 aatactttgc cactgacaat ggccgactgg tccgtctggc accaaccagc cagccggcct
    38701 ggagtcagag cgacgtcatg aactttggca gcatgacgct gagcgaagcc ttcaatctcg
    38761 attttgtcca ctaccgaaaa caaatcacga cacaggcccc ccgcttttcc gaagccggct
    38821 ttgccggcta tgtgaatgcc cttcaggcct cgaacattct cgacactatt aaaaaggaga
    38881 aatttaacct cacaaccact atcggggcag gtaccctggt caaccaggga cagatggaga
    38941 acggcgtctg gttctggaaa ttccagtatc cggtcaggat gcgcctggtc ggtcagacca
    39001 gcacccgccc cgaacaggcc ttcacctttg aaatcactgt ccggcgcgtt gacccccgca
    39061 tcaaacccgg cggcatggaa atcagccaga tggtttcccg taacgccgca gcaggaatct
    39121 gagactcata tcatgcacat acatcatctt attggcgcac tgcttattgt gtgcggcact
    39181 gtcacagctg cagataaccc gggctggaaa aatgcacgaa gcccgcagac cggcggaaag
    39241 accgcacagg caaccgggaa caccccggca ggcggtcccc cgccagccgc acaactcccg
    39301 ggaaacctgc cgcctcccgg cgctgctctt gtacaggaag gcgcatcgct ggattctccc
    39361 ctgaatgcca gtgaaattcg tgaactccgt gggataatgg ccgataacga acgggccatc
    39421 agtgccccgc tcaccagtgt ggtgccccgt atcagttccc tcacggtgaa tctgtctccg
    39481 ggggccagcc tgccccttgt ccgtaccgct ctgcataacg tcagcgtggt gaccttcacg
    39541 gacgtcaacg gcagtccgtg gccacagtcg gatccgccct ataacgctgc gcccggtcac
    39601 tttgacgtgc agttcaacgg caatatggtg tccgtcaccc ccctgcgcgc ctgggctgcc
    39661 gggaatgtga cggtctatct caaaggattg tctgtaccgg tcatcctgaa tatcaccagt
    39721 ggtgaaacgg acaccactac cctgagtcag gaaatcgaca gtcgtctgga tctgcgcatt
    39781 ccccggcagg gacctgccag cccggtggtg tccataccgt cagaaaaaat tgccctgcat
    39841 gacgccactc tgcaggcctt tctggacggt gtgccaccgg atgatcccgc catccgccgc
    39901 ctgaaattct ccggtaacgt cccggacacc accatctggc aacacggcga tgatcttctc
    39961 gtgcgctcac gggccatgct gcgggatgaa tttgaacaga cgctggcatc ggctgatggt
    40021 acgcggctgt ggaaactgcc ggtgaccccc ttactcacct tctccgtgaa tggtcagtcc
    40081 gttcacgtca ccccggaact ggagtaaatc atgagcgcag aacaagaaca ggacacagcc
    40141 gcaaaatccg gcaaaaagct gtcagccctg ctggttatgg ggggcgtgat tatcatcggg
    40201 ggcggatacc tgctgctcac ctggctgtcc cccgaaaacg ctgcatcgct ctcgtcagtc
    40261 aatatcaaca gcacaacgtc cggcggcgga cggaacgtga tggagacaca gcactaccgg
    40321 gatttactgc gggctgacaa tgaacgtggt gctgcagaag cgttacgtaa taacgagacg
    40381 tttatcgcca gtctgccccg gggactggat accgataccg gaaacgatca gaaaagggat
    40441 aatccccccg atgatcagtc aggcacttcc gggcaaaacg catccccccc tcaggaagat
    40501 cagactgccc gggaaaaacg gcgtgaggca ttacagaaac tgcttgcccg tatcaataca
    40561 cagcatcagg atgcccggcc accggtagtg gcagcagcca tgtggagcac caccggcact
    40621 cccgggacag ccccacatgc gtcaccgccc ggtaccgtta catcacaaca tccccgtctg
    40681 caggagacga gtctggcaac cccggcagca cgtccgggga tccagctgat accggcactg
    40741 acccgcgtac cggcctacat tgataccgaa gtggactctg acaatgcggt ctcccgtgtg
    40801 gtggcctccg tacctgccgg cccatgggcc ggggccgtcc tctacagcca ggacgccaga
    40861 ctcgcaggtc agggaatgga tatccacttt gaccggatga tgtggagggg catgtttctg
    40921 aaggtgaatg catatgccct gaatgaacga acaggaatgt caaccgtggc cagcgacgtg
    40981 aaccaccgct ggtttaagca catcgttctg ccgtccgtac ttggcggggt gggcaatgtc
    41041 ggaagtcttt acaaagacgc caacacacag atccttcagg gaaactacgg cagtattacc
    41101 ggcagggtgg gaatgcctga tggagaggcg ctggccggcg tcattgcagg tggcatggcc
    41161 gaacggggca gccagctgct gacccgccag gcagagagcg aaccttaccg tcaggtgaca
    41221 gtatcacggg gcgaggtcgt ctccattctg tttgttgagc cggtcatgag caacgatatc
    41281 agtacgccgt cctccggcac tgccccttct gagtccccct cctccggacg tccgctcacc
    41341 caggcggagg cagagcacca ggcctctgac aggatccggg cggcggtcct ccgtaaacag
    41401 aatgaaatgc agcaacgcta cggcatacaa caggaaagtt atccatgaaa aacgaacatg
    41461 aagatcccgg cagctttggc gaaacgacgg caccggcatc cggcaaatcc ttcctgaaac
    41521 gccagtatat gggatacagc ctgccggtct gggccggctg tgccctcatc atcctggtcg
    41581 ccatctggta caagtttctg cggacaccct cagagccccc tgcagatgca ggctttgctg
    41641 tcacaccgcc cgctgctgag agctgggcag agcccatgac catgacggag tccgggagcg
    41701 gaaacaacat gcccgcaccg ccggttaccc cacaggcaga catgcagcaa cttgcccgtg
    41761 atctgaaagc agagctggac agacgggatg aggaaatccg gcgcaccctg accatgctga
    41821 aggacagtat caccaccctc agtaacgcca ttcagaagga tgaggcctgg gcacaggaaa
    41881 cccgtcagtt gcttgaggag acccgtaagc aggtgcatac cctgcagatg cagggagcac
    41941 tgccagcccc gaccgggaat aacagacagc ctgcgaaaac cctgccttca cctctgcagg
    42001 gcatgaaaat catgtcggtg gagaaaggca tggcctggat ccgctggcag ggcagtgcct
    42061 gggcagtacg ggaaggcgac agtctgggaa acatcaccat cacccggatt gacccgtcca
    42121 gccgggcagt catcacctct gccggtacat tacgatgatg agcattgatg ccctgacagc
    42181 catagagaac tttgccagcg gtattttcag tgcaggcatg gagtttctct ttacctgggg
    42241 agagctgctg gggattatcg gccttatcgg tcatctgatg cgcgcccggg cagaaggacg
    42301 tcacagtatg ggaccgggaa aatttattgc cggtatcttc atctgcggca tgctggtcag
    42361 tctgccctca atgatcaatg ccggtggtac ccagatgggc ttcagggcag attctttcgc
    42421 ccccatcgca tacgtgcagc cgggctcctt tggtgccgct gccggtgccg tcaatgccat
    42481 tctctcactg gcaaaactgg ccggcgtcgg ttttgtcatg aacggcatat ccatctggcg
    42541 caaatcactg ctggacgggc acaccgccct gagcgccagt gaatccatca gtaaagggaa
    42601 cgtcaagttt gtggccggcg ttctgctggt tttcaacgac cgggttatcg atgccactct
    42661 ggcaagcctc ggtatcgcct tctgacccct gtattttttc agtaaatcaa tctgttaata
    42721 aaggagacac tatgtcacga cttcgtgcca tggcggcgcg tctgcgcgct gcatctgttt
    42781 atgttcagtt atgcctgatg gcctgccgtg accggctggt acgcctgtta ctgctgcttc
    42841 ccgtgatgct gctgccccac accgcaatgg caaataccct cttcgatatg attaaaaccg
    42901 ttggcgatgg tgccagaggc tccagcagtt cgtttgtggc tatcggtcag gttattggcc
    42961 tggtgctctt tatcggcgga ctgcttggcc tcaaacaaat cggtaaaaaa cccggtgcag
    43021 ggctggcctg cctgctgtca attctggtcg gcgtctgcct tgccgtcgta ccggaactga
    43081 tgggcagcgg acagaaaacg ctcggtcttt ctccggtttc aatcagttaa tccgcaaagg
    43141 caccgggaaa ccggtgccgt cttcacacaa tgaggaggac cagaccatgt cctgtgagct
    43201 accccctttc accctgctca gaacagagca cggcaacgtg attgcccgcg acaaccggga
    43261 taatcaggag tttgtgtttg cagagtgtta ctccaccgct atagcagcac tgattctggc
    43321 tgccgtcacc ctgtatacag agagaaaagc gtatgaaaaa gaccccgcca ctcatcgccc
    43381 cggtccggga acgatggctt ttcccgctcc cgcaaccgga cacggtattc agcggtgaag
    43441 ccctgttacc accgccagtg ctctcaacag agggacgctg cccctgctgt cgcaggaccg
    43501 tcacccaccg cttcattctg gaagattcat ggccattaca gcagatggct gacacctgcc
    43561 aggatacgat cgtcctgctg gaaaaaaacc tcacccgggt gatgcgcctg aaaaaacgtc
    43621 cggtaccgga aaacgctgac gaaaaaaaga aacacacccg aaccctgcag gatgcggagc
    43681 ggtccctggc acaggcccgc ctgtcggcca gacgactggc cctgcgtcat gtggaaaagt
    43741 cgcaaatcgt cacaacggat gcactcagcg aaaatgaaag tgacctgctg cagccggaag
    43801 gcccgccctt tcatctctgt gccttctgcc acgcctggca ctgtctgaac ggctacgctg
    43861 ccgcacaggg agtgatggtc tggctccccg atttgcatcc ggcctcggtg gttgccctca
    43921 atgcgcgggc actgcaggag attttcagcg ataaccgtca gcgggtcagg cagggacggg
    43981 cagtcctgaa tgccctggta cagaaccgtc tggccgttga ggagaaattc cgcacctggc
    44041 gtccggcgga ttttgctgat gccctgcgcc gctggccgcc ggaacagcgt aaaacgttga
    44101 gggagaaaat ggacggggtg gcgctcattc tgctgccgga ttcgtttcct gacaaaaagt
    44161 acgtcatgtg acacacaccg gtgccggtga cgttttgccg gatatcagtt ctccgcagat
    44221 gcattccggc actttattat ccggtcgctg actgacgata ttccacctga cggagatgaa
    44281 aaagagattg cccggttcat tcgccgccag cagtttattt atccgggtgt aatcacacag
    44341 aaatacgccc ctgcccctca acatattatt tatcttctct gctgtttttt catgccacat
    44401 cccctgctgt ggcatcaaaa aatataagac cgtgatgatt catccttacc ggagtgcccc
    44461 atgccatgaa tatcaaccgc attatttttg ccattgaaga ctgtatcagt accctgtcac
    44521 ggtattccgt ttccgcattc ttccccgatt actgtgatct gcacaccgtc atcggtctgg
    44581 accagcagga ccgcgaacgc cgcccctggc tgaaagcccc ctacattgcc accacaaaac
    44641 aggggtatta cctttccgtc tttgaaatca gcggtgccct gaaagaaatg gatgaaaacc
    44701 cggaccagac ggcccccggg acactggagt cactgattgc agcactggct gagcgaatga
    44761 acacggcctg gaaaaattcc ggccacaaaa tcagttttgt tttcgaacgc gatccggagc
    44821 gtggaaggga tgaaatcgaa gccatgctct taccccagcg aaaatccatc gcacgcacgg
    44881 ggatcctgct tcaggacgtc ctggatgaaa aagtcaccac actcaccccc tggcttgtcc
    44941 gggagcgctg ctggctgacc gtctggagct ctgaagaact gctgagccgg accgatcgca
    45001 aggaccatca gacccgtgtc aggaaactcg ctgaacacgc tccgccagcc cgttttgcgc
    45061 aggatccctg gcgctggaca ctttccgccc tcaaaatacg gcatgacgcc ttactggaca
    45121 cgctggagca ggccctgaca catgattctg atggcctgct gattcgcctg atggatatcc
    45181 atgaagtggg acgggaaatc cgccgccagc tggagcgcaa cagtacaccg gcagtatggc
    45241 agcctcatct gcctgaagat gcccgtcctg ccggctggcg acagggcggg gatacctctg
    45301 tattccacgc accgtcgctg aatctgcagt tattcaccac acagcctgaa acacacggca
    45361 gcctgattca ggccggtgag ctctggcacg gtatggtcgc cattaccctg ccccctcaga
    45421 atctgcggac attcaaccag ctggtcaggg atgtgccccg tgcaatcccc tggcgtatcc
    45481 ggatggatct gatgcccgac ggcatgaagg cgctgggggt caagaaaacc ctgctgacct
    45541 acagcagctt tattcctcct ctccggccca tgtatgactc ggtaatgatg ctgaatgaca
    45601 tcaacaagca tgatccggtc tgtatcatga ccattgttgc caccacatgg ggaaacagcc
    45661 gggaagtctg tacccgtaac caggctctcc tgaaatctgc gctggaaggc tggggggtat
    45721 gcggcaccac caccaccttc ggtgatccga gacgtgcctg ggtgaacacg gttctggcag
    45781 cctcccacag ctccggcccg atcccccttt acccgcccct ttcacacgcc ctttccctgt
    45841 ttcccctgaa cagggcaggc tctgtctggc ggggacaggg aaatctgatg atgcacaccg
    45901 aagacggctc tgcatgggag gtggcgctgg cgtcatcaca acagaacaag cacacggagc
    45961 tgacacccgg cgcacccggt ctgggcaaat cggtactcat caacgcactg tcggagatcc
    46021 agatagcctc agcccagaag aatctccctt tcattgccta tatcgacaag ggattcagtg
    46081 cacagggact ggtacagctt atccgtgaca gcctgcctga gcagagaaag gacgaagccg
    46141 tggggatcat tctgagtaat gacccggacc acacccgcaa cctgtttgat gtgatgtacg
    46201 gggccagaaa acccgtcaca ccggaaaaga acttcatggt ctccgtcctc tgtgcattgt
    46261 gtgtggatac cggcaccggt cagccctgta atcccggcga cacccggcaa atcatcagca
    46321 gcctggtgga cctggccttc cgggaatatg gcgagaataa ccccagactt taccgcgcag
    46381 gtacggaacc cctggtggat ctcgccctgg aagagagcgg catcgctgaa caacatgatg
    46441 ccggatggtg gaatgccgcc acctggtttg aaatcaggga catgctgcat attgcaggga
    46501 atatccccgc cgcccagcgg gcacactacc aggccatgcc cctgctggct gaaatgtccg
    46561 ccctgctggg acagccgtcc atccgggatg tgttcggcac cgttcagcgt gataactcgg
    46621 aagagcggtt gctggactac atccgccgtg ccctcgatca gggacacagt gactacccga
    46681 tgatgtccgg ctgtacccgg tttatgctca gccccgacac ccgcgtggtc gccgttgatc
    46741 tgaacaatgt tgccggggac aagacacctg ccggacgtct gcgcaccggt atcatgtatc
    46801 tgctggccgg gcagatcgcc ggcggggatt ttgtcctgcc gcagtatcag gaggaaatac
    46861 ggaaaaatct cgacccacgc taccatgagg tgattttccg ccgcattgaa cagcttgatc
    46921 aggaggtcaa gaccaaagtc tatgacgaac tgcacaatgc aaaagggatc aacttcatct
    46981 gggaagccct ggacacccag gagctggagc agcgtaagtt cgggatacgc acggtgctga
    47041 gcacccagct gctgcagcat tatcctccgg ccgtgctcaa atccgccaac accctgtggc
    47101 tgctgcgtta ccgccctgac gaaattccgt tcctgcggga taactttggc gtcccggaag
    47161 tcacactgcg gcgcttcctg aaaatgccgg aaggggccgc accggacggc agcggtgtcc
    47221 cggtactggc tgtgttccgt gtaaaaaacg gcacgctggc ccggatcctg aaattcaccc
    47281 tcggtccact ggagctgtgg gcactgaact cctctcctaa agacagcgcc ctgcgacgtg
    47341 cgctgacgca ggaggtgggg tcactgcggg cacgccagat cctggccgaa cactttcccc
    47401 ggggttcagc cacctcactg atagaacacc gcgcccgcac acatgacagt gaaaacgtca
    47461 tccatgagct ggcagcagaa ctcatccgta agcagggata taacctctga tgtacagcat
    47521 tcatatcacc ccccgctgcc ggcgtcctgc ccggggacag gtcttcctga aaacactgcg
    47581 cgtggacggt gacagtttca gcttcaccct gccgtccgcc tgcttcacac tgcgaccgtc
    47641 ccccaatccc cgggtcattt ttgccagtga cagacgggct gacggggaga tgagtggtat
    47701 tcgcctggcc ctgcccggag agacaggcac gctgataaca acggccacct ggcaggttaa
    47761 cgtgccggag cgaccgcctt ttgatataca gcatgtcatt cactggcttc tgcccgcacc
    47821 actcactgcg ctgatgtctg acaacacctg gctgtggccg gatgtccccg gcacaccggc
    47881 actcacagcc ggagactggc cggtcatgga aagcgacacc ccgacactga aacgcgtgaa
    47941 gcgtcgtacc ttcacgctca tcgataaccg taaccgtcag ggatggatca cgcgccgctt
    48001 ccagtgtttc ccgctggctc cgctgccgga atcactcagt catcgcgtac cagacatcct
    48061 gtatcccccg aaaacggaga aaaaagaatg caaagacaat ctgcaaccac actgcttgca
    48121 ctccttgtag gctgtgcggt tatccctgcc acccatgcct actctgtgaa tgttgtgtcc
    48181 agtcagccgg tcacccgaca gatcatgccg gagctggcca ccatacaggg catcatggag
    48241 gaaatggtcc gtatgcagac ggccaccggc acagccatca cccagaactc agaaaaactg
    48301 gccaccgtca ttgcccagga cgggcaggcc acccgtcagc agatgatatt cagtaatgaa
    48361 acacaccgcc tcgaggaggc caggaaaagc ttcagcgtgc cggactccat ctgcagtgaa
    48421 tccgcgtccg gcattgcagc agaaagccgc cgggcagcag cgtctgcagc ggcacgtctg
    48481 agtcaggggg gaggggtcag cagtaaaccc atccgggaac gtctctcccg ggctgcggac
    48541 tccccggtcc gcgaggcgta tgacagtgcc ggcatccacg ccggttactg cacggaagcg
    48601 gagtatgccc gctttggcgg aactgatgtc tgccctgccg tgggcgatct gccgggcgga
    48661 gacagccagg tacgatcgct ctatcagggc gcaggcacag cagatacccc ggccgctctc
    48721 acctgggatc agaaacagat tgatgccgcg acggcttata tgaaaaacac cgccagaccg
    48781 tcagcgggtc gtgccccggg gaaaggagaa gtgggcacac agaccgggcg cacgtatgtg
    48841 ggcctgcaga atgaatacaa cggcatcatc gatgccgcct ctcatccgca gctgtcactg
    48901 attgctgaca gtacccctaa tgaagccacc cgtggggcac tgactgaagc cctgcagtca
    48961 ccgtcagctg cggcatattt tgaccggacc gcatcatcag aagcccgcac ccgtggccac
    49021 atgagccagc gggagtttga ggcctttgaa gccggcagac ggtatgccaa caccgactgg
    49081 cagcaggatc tgcagggcat ggaaggcgat aaccttctcc gtgagctgct gcgcaccacc
    49141 gccctgctga actggcagat gaacgatctg aaagagcaga ttcgtcaggg taacgttatc
    49201 gccgggcagc aacttgcgct ggccgcacgg cagtattacg gacagcgcct tggcgaactg
    49261 tcacaggcca tgagccaggg gagcgtaaga tgaaagacga caacagcatc gacgaaaaaa
    49321 aaccggcccc ccgtctccgc cgcatcggtg atatagggat aaatctgttc gcaccactgg
    49381 ccgaaacccg ctggatgctg cgcagctgcc gccatatgct ggaccagcat aaaacgcgcc
    49441 tgaagcgcct catgcccgac cggcaaccgg agagtccggt ctgtatgacc tgggaggaag
    49501 cggtcagggc cagcggactg acggtcgacg aactggaccg gcgtctgcga cgtcgcagga
    49561 tgttctggcg gacaggattc tggctgatga tgataccggt acttctgctg tgtggtatgg
    49621 cccttgcggc caccgcactg ccggtcatca ccctgatacg tatcctggtc tgcctgttac
    49681 tgatgagtac cattgcagca ctgtgtgcct caaaagccct gattgcgtca taccgcctct
    49741 ggcagctgaa tgaacgtcgg gtcagcgagg acgaacaggg cacattcccc gattacctgc
    49801 aggcccggca cggctggaaa caggccacat ggctggccat tacccaccgg cagtactgac
    49861 tgtccggtct gcaatttctt ttcctcattc cgggggctga tgcccgggca tccccttcgg
    49921 ttccggagtt accgtgaaaa tcatacccag agcactgagt gcaggtctgg cgctggctgc
    49981 cgtacctgca caggctttcg tcacctatca ggacattgtg agtgccgcca ccaatccgga
    50041 tgacctgtcg cgccaggcac tggtcaccat ttttggtgat gtggtcacaa atcccctgac
    50101 agccagcagc aacaccatca ttggcaacct gttcatggtg tttaacgcaa ttatcgccac
    50161 gatggggata gtctggtttc tgtttattgg tatccgtcat gctgtccgca cgggacacca
    50221 gggcagtgtc tttaacaacg gacgggacgt cgtcggcata ctcagtgtcg tgagtggatt
    50281 tttgatgatt gtgcccaccg cttccggctg gagtctggcg caactggtca tgctgtgggg
    50341 cgcatccatc atgggcatcg gttccgccaa tatcatggtt cagacagcgg cagaaaacat
    50401 tgctgaaggc tattcaatga ccgtacagcc agtacatgtc tccacccgca cggcagcacg
    50461 gggaattctg gagatggaac tgtgcaaaca cgccatgaat acgggcctcg atgactttaa
    50521 ccgtaccgca cgttcatcaa cgccgcccat gagcgaatcc tcacggacag agaatggcag
    50581 atataccgtc accgtctcaa atggcagcgc cacctgcggg agtgtgagtc tctccgtgaa
    50641 aggaaacggc agtaacaagc agtccgcgct ggcccatttt ttcaatccct tcattgaaac
    50701 ggaatatcag ggggttctga cggcacaacg ctccgccatg gataccatgc tccgtgacct
    50761 ggacagcgcg gcgcaggaat ttgtgtcggc ataccggacc cggcgcgaca cgggtaacgg
    50821 caacttgccg gatattgaaa gccgtattca gaaggcagcc aaccggtatg aagaaacggt
    50881 gcagaaggcg ttgccggcag ataacgcaga aactgagcgc agggagcagc tgaaatccta
    50941 cctgaccacc tacggctggg ttgccctggg tgcctggtac cagacctttg ccaccgccaa
    51001 ccagcgactg gaagacctgg ccaaccgcgc tcctactgtg agtggcgtct cgtcactggg
    51061 cgaagccggg gatacggaat tcctgaagga agtcaagggg gcttaccagg cacagctaca
    51121 gaactcagcg tatactcctg cactgggaac agccacctct gccagtgaat accacgcatc
    51181 taaaactcaa gaccctcaat cagcaatctt aaaattgatt gggtttaacc taacaagaat
    51241 gactaactgg acagccactt atctgagcgg tacaggtacc acaactaatc aactaaatcc
    51301 gttgataaaa atgaaaaaca ttggcgatta caccatggtt acgaccgaaa ccatctgggc
    51361 gggttatacc accgtcaggg tactttccac ggccggcagc aactccgttt ttggtcgact
    51421 ggtcaatgcc gtcagcggat tatctgacgc cgcaaaggct tttctggaag catccgcacc
    51481 accactctac ttcctgctgt ttttgctgtt ttgtgccggt ttttcgttat ccatttacct
    51541 gccgtttatc ccgtttatct tctggatgac cggcatcggc aactggatag tcagcgtact
    51601 gattggatgt gctgctggcc ccctgtgggc cgccacccat ctgggaacaa gtcaggaccg
    51661 gggaagccgg gccgcttacg gttacatcta ccttatagac agcatgatca gaccgctgct
    51721 gatggtgttc gggttctttt ttgcatcagt cgccatcgtg gccggcggca caatcctgaa
    51781 tgcgctgtat gctgctgcac tgatgaatgt ccagataaac tctgtgacgg gcgtgttcag
    51841 catggcggga tttttgatga tttatgcccg cgcctgtacc acactggttg cagccgtttt
    51901 tgcgcttcag gcttatctgc ctgaccatgt aattaatttt ctgggcggac gtgacggtgt
    51961 taatatgctg gggaatatgg tttcttcagt caaagatatt attgccggag gtaataccaa
    52021 tatgcgcaga acacctggcg ttcgtgaaga aaaactgaaa aatattaata gagatgataa
    52081 agacggaata aaacaataaa tgaaaatatc gaaaaaccac taaaacacca ttgccatatt
    52141 cacctgaaga gattaaaata cattcaggtg aatatggagg tttattatga acacaacaaa
    52201 aagcagatat ccttcctggt gggcatatta ttaccttatc cgcactgctt attttatatt
    52261 tggtgttcct tttttattac tatgttctct aggatttacc ggtatgatgc ttacagaacc
    52321 atatatagaa acagatattc aagcatatct atatacttat ggcagttggc tcctattaat
    52381 actcccatca ctctggctat atatgaaaac cagagcgaag aaaaagaaag tccagcaggt
    52441 tgttcagcag ataaaagaaa gcagtccctt tgcgccgacc agcgattatg agcaactgtc
    52501 attctcgaag agttgttatt tcgggatcga cattaaaaac ggcaccatgc tgtatgtgcg
    52561 catttatccg aacaatgtga tggatgtgat tggtctggat attcacaact ttacccgcac
    52621 ggttgctgaa gacggcaagc tggaaatcca cactacatat gtgagtctgc cgatgatccc
    52681 actggaaatc tccggcatct catcaaggac gctggccaac accatgcaca ccatggctgc
    52741 acgtggatat gagtacaagg aacgtttccc acagatgatc cggtatcggg ttaaagagtg
    52801 ggaaaaagtt gccggtgttc ctgttgcaga agtattttga ggtagctatc aactgttaga
    52861 cgataatccg tcagccggtt ttctctgaac cggctttttc ttttcaggca gaatgcctgt
    52921 ctgccgtcta tctcaggacg ccgcatggag tctctatgtt ttccccacaa attcttagca
    52981 ctgtcttgcc cactgtagac aaaatcaata caattctgca tatcagttat ctataaaaga
    53041 taacaatatc attataaata attatcatat ccacacactc aaggcagtac ctagaaaaca
    53101 taaagataaa atcatgtctg aatcccgcag caattttcca gcacgacatg gtactccatg
    53161 gaatactgaa gagttacagc gtcttctcgt atccatagcg gagggcgtct cgctaaaaaa
    53221 acttactcaa acacatcaac gatcgtccgg gggaatacgt ggagcaatca aacgcctgct
    53281 tccttctgag tttcttgagt tcaacgatat aaacacggtg aatgacctca gcaattactt
    53341 caacaagatt cctcgccaag aacacaatag tatcatcaat aatttaatat cattgagcac
    53401 tgcaaaaaca attgctcatg ataaagttga aatacaaaag aatagtttta aacaaaatac
    53461 cccactcccc ctctgccaac ccaatacaga ttttaaagat tatatagccg atgaacatga
    53521 tgtcattatg ctaattcatt ctgctatcga aagtttatct aataaacgac atcaatatat
    53581 tcttctaatg cgactcggca cagatgattc acctcattct ctggcagata ttgccgtcaa
    53641 attaggaatt tcccgtgaaa gggtaagaca acttcaggaa cgagcccttg gcttactagc
    53701 tgctaaatct cgctatgaag ggacacctgg agcttgcctt aaagccctta ttaactcaat
    53761 aggtaattca ccatatgata tagcagtatg gatttatcgt attgttcatc gggatttcgt
    53821 tacatcctct gagttagccg ccaaattcat catttttgcc gcaggcattc ctaaagcaag
    53881 acgagatgaa ataaaaaatt cgctctcatt aatatcacaa ttacaaaagc agaagttacg
    53941 tgaacgaaga gcagaattag cacacgctca cgctcaggaa agagcccgaa tcaagaaaga
    54001 aagagttgag ttcatattca gtaaatggct agacaataca cattggccaa aatatatcgc
    54061 acctcctccc cccgtagaac agctatgttc acagagagcg gttaatgata ctgacatatc
    54121 tggattcttt tactctcaaa aattaggtcg tacagtccag tacgaatcag ggcttgagtt
    54181 taaaataatg aatctattag aatgttgcga tcaggttctt ttctatcaag aacaaccatt
    54241 ttctattcca tattgtttta aaaacaaatc aaaaaaatac tatccagatt tactcattgc
    54301 gacagttgat ggtcggtgcc tacttatgga agtgaagcca acagaccgaa tgaccttgag
    54361 tctcacgaga ataaaaagta atgcaggtcg caaatgggca catactcgag gatggggatg
    54421 gttggtcatt agtgataggt tctcgttacg tcagctggaa gaacatgcca tctcaacaaa
    54481 cacatggaaa cttatagagg ctgaactgaa aacatcaagg attttagcat ggcgtgaaat
    54541 gatggagtta cgtacacaat acgagattca tcgtttagat cttattgcat atatcattca
    54601 gtctggtgct gaagtggata acttttatcg aattaccccg aaaatatcta ataatacatc
    54661 taaccagaga caaccaaatg agagcgactg tcataattga tgacgcacag gacggaaaaa
    54721 ataatagctg ccggacagca gcaaccagca gtcgtccggt aaaaaagaca aacaaggcgc
    54781 attgaggtca gccttcgcaa caatccggcg gcagatgtcc tgccgccgtg ttgcccgtta
    54841 acgtttaact tcgtaggcta acgttgccac aaacaccgta tttccctgct cgatacgaaa
    54901 tccgcaaagc gaatccctca ccatccagac aaaacacagt atcgtcacac agacgacgat
    54961 taacatcatt aaaaacgttc gttgtggcat cgtagcccct ccattgcctt tcggccgtca
    55021 tcgggctact ataatgttgt ctaggcatta cagtgggccc tgggttgatt gataaaaaaa
    55081 tcgcctgggg ctttctgcta catgccgtcc acaaaacgct ttcggcatac agcctaaagc
    55141 gcccgccgct attctaatcg ttttcctgtc cgccacaatc cttttcatgc caccacgtat
    55201 tttgtgtcag ggacagaaat aaagccggaa agtgactacg tgtgcacaca tctgttttcc
    55261 gggctgcagg catcgtgtta ttttctgaaa tgcaaaataa taaaagacaa ggacacatca
    55321 tgaaggatga aatacacagt accagcatta acccggatgt tgttgagcag tttctggccc
    55381 ttatgaatac acattgtaaa tcctcagaag aggcgctcac catgctgagt acgctttgtt
    55441 acaacctgat gatgacatgc ggtatccatt ccatggacag ccagatgctc aatgggcatg
    55501 ggatatccat gcaacttcag gggattgata ttgccgaaaa gccaggcaag aatattcact
    55561 aacctgaaca gggcctgaaa acgctgtctt ttcaggcctc tgcaggcaaa aactgatgac
    55621 aacaaaattt ctgcgtttgt accccaccga tgaaaaagac aaaaactcac accgggcttc
    55681 tcatcattaa agataaaacc cgacgtgtca gcctctacga aacgccaacg gcatggtgta
    55741 tcagggggca ggagtgctac agcaaaagca ctggacgccg ctgtggcagc cacgacagtc
    55801 tgagcagact gcgcctggac agcattaagc ctgttgaatg agtggtggca ccatgaaaga
    55861 taacaacaca accgccaccc cagaaacctg gaaaatgcgt tttctggcca acgggacatc
    55921 cggcacatgg ttttacactg acgacattgc agtcactgac gcgtggcttc gcgcgcaccc
    55981 ggatgatgtg gaggttatcc ctctctgccg gatacagccc cgccagcatt cccatgaaca
    56041 acatattgaa gaactccgcg cagccctggc ccggatgctc catatgcatc atatgatgct
    56101 ggaaaccaca cattaccggg aaaatatcac cgatgcccgt tgtatccgtg aaatgcatga
    56161 agcgccatta caggcgctca atattctgtc cagatacccg cataaaaagg acgaggtgtg
    56221 acgcatgtgg ctactgattt tctggctttc atcgcccttt aatgtctctt ccggtgatgc
    56281 ccgtcagccc acactggcgg tgcacagcca ggcctttcat accgaacggg cctgccgcga
    56341 tgcattgcgc gccattaaaa aggagaatgt tggcgaattt tctctgcggg gcgtttgtgt
    56401 ttctgacgga aatacgaagg aggaggctga gagctcatga cggatattac tgacagacat
    56461 acacttcgcc gtatgtcctg gtcggaactc atcacggcag gacaggaagc agaatttcag
    56521 catgattacg agcgtgccag aatagtgtgg tcattcgcct tacatgttgc aacaaccacg
    56581 attaacaaaa atctggccat agctcatatt cgacgctgcg atactttgtt gcataaatca
    56641 aaggctgtac ccggaaacaa cccgtgtggt tccggtcagc gagtctgaga cctcaacatc
    56701 ccgggtgatg attgtcattc cggactgacg cagccgctgc acatctgcgg caatacgttg
    56761 aataccaagc cagaacagac catccagcgc ggtgacaatt aacccgcttt ccagcgccag
    56821 tttcagccgt gctgtcgggg ctttaacctg cccggctata tcagtgaaac aacgcgtcgt
    56881 catgccatag ctgtccaccc gccgtattcg cgtcgcaaga ctgtaactga agctatccgg
    56941 cacgccggat aagtcattct gcagactgta tacacatccc cgctgccgct cattgaatac
    57001 gacttttttc tggctgagct gccattcccg ccgcacgctg tcaatcatct gcggtgtccg
    57061 ggttaacagc agacgaaacg gcagttcaaa actggtcaca taatcaacat taagtaaggc
    57121 aatccgcagc cgctccctgg ctcctgctcc ggtatcacgg cattcacgca tgacatcttc
    57181 ccactggcgc atcagtcgcg gtgactcatt cctggccctg aactgatatt tctcaatctg
    57241 ataatcaata tgttcctgca taccgtcacc ggatatctgt tgccgtatca tcacggatat
    57301 cagcgtactg gaattcatgc cctgcgtgaa gtatcacact gccaaaaaaa aacacctgaa
    57361 cgttattatt tcgcaatatt aattgcacct gttttcataa ataaaaagga gacgatgaaa
    57421 tgataaaata tatcttttca ctcaccatta ttttttccgc ttctctctgt gccgcacaga
    57481 ttcaggggca cgtcatccgc gttcttgatg gtgatacgct ggaaatatta caggacaaaa
    57541 agcctgtcag ggtccgtctg gctaatattg atgcaccgga aaagaaacag ccctttggac
    57601 gctggtcagc cactcagctg aaggacctag tggccggaaa gcccgtaacc gtgcaatacg
    57661 cgcagacaga caggtatggc cgtattctcg gccgggtcta taccgctgac ggcacagaag
    57721 caaatcgctg gcaggtccgt aacggcgctg cctgggtgta cccggactac aataatgact
    57781 atactctccc gccgctccag cgtgatgccc gcgccctgaa gcggggatta tgggcagata
    57841 aaaatccggt accaccatgg acctggagaa aacagaataa gaaataatta tttgctgata
    57901 tgaagaaaaa aaccatttca aagaaaataa atggcaaaaa tacagcatgg aaaaaattgc
    57961 catttttacc gccgcgttat ttttaatatt tgctgtctgc tggtcgtaat aagcagaata
    58021 acagaaaggt gtaaaaatga acctggttga tgcatttgtc actaaagtga tatccggacc
    58081 gtatgaagaa tacggaaaat ggtgggtgga tgtagaatat atttcctggg gagttccggg
    58141 taaaagccgg ctcatgtttg actcacggga acaggcacag gaagtgcagg aaggttttcg
    58201 atttttaacc tgagcatatt ttgttaaagg gcagaaggcg gtgaccctct gcccttaagt
    58261 acgtttccgg gattatcagt caaccagttc ccggatcagt tccaggagag ctatcactgc
    58321 gttagccaca gcaacaaccg ccctgagaac gatgattaac aggttaatca tccgcttctc
    58381 catcaatgga gaaagaacac cgtggtttgc ccttacattg cctgtcgcca cgttttctat
    58441 agtgtcagtt atagacagtt cttctccggc cagagcacca aaaccagcac cacctgatgt
    58501 tttggccagg acttgcaacc tgttcatccc ggtgaacgat atgccggaaa gaacgtgcct
    58561 caaagctgac acaccgagta tatacttata ctgtgcttgc agaaatatta tttcagccgt
    58621 attatttcat agtcagttat aggcagttat cttccggtca gggctaccaa cttcgatccg
    58681 gaatcgcaaa aaaaccggcc tcaccagtcg gtttttttgc gtctgtacct cctccccgga
    58741 cagcagttct ctcactcccg ttgaatattt cctgttgaat tttacgaaaa tacggcaaat
    58801 gggagatatc gaatatattt acttgggtgt agtcggtaaa aaccagctca tgtttgactc
    58861 acagaaacag gctctggaag tacagaaagt aatctgtccg tagattatta aagggcagag
    58921 ggcggtgacc ctctgccctt aagtacgttt ccgggattat cagtcaacca gttcccggat
    58981 cagttccagg attgctatca ctgcgttagc cacagcaaca accgccctga gaacgatgat
    59041 taacaggtta atcatccgtt tctccatcaa tggaggaaat cactgttgtt tactcttaca
    59101 ttgcctgtca caacagctct ttcattgtta gctgaaaaga gacttcctcc ggccagagca
    59161 ccagacgcag caccacctgt ttgtccggcc aggtctttga atagcacaga tccatagtcc
    59221 cgatggattg atgcgaaggt aagtctccag acaaggctaa cttgctgatt atacttgttt
    59281 caggcgatat cgctactccc ggaacaatag actgtaacga acagattgcg atcaggtgaa
    59341 tataacgata ttctggtaac gttagttgaa aagagacttc ctccgggcca gagccaccca
    59401 ctctccctgg gatcgcaaaa aagccggcct caccagtcgg cttttttgtg cctgtctctc
    59461 accaccttct gctgttcctg tgaaaatgcc ctgctgaatt cagccctgcc cagtcattac
    59521 cggcgattgt tcgcattaat tccactcagc ctcatcccga aatttatcta tttatcgatg
    59581 gaggaatccc cgtgtcttac caacagacgt ctgccgccga agatccaatg gctatatggt
    59641 atatcgtcgg tgccatatgc ctgctctttg cgattatcat ctggcgcttt ttacctgaaa
    59701 tcgtcttcgc ctcctgcctg atcctgcaca cactgtgggg catgattgac tgggggccct
    59761 ttcacaactt tgccgccccc cgttacaacc tgctggccat cactgctaac aacgcggcaa
    59821 ccatcacttt cggccagtgg ctggatgtta tgtcccggac agtggggatc ctctggttga
    59881 ttctgcttcc catgacattc ggctttctgt ggatgtggtt tcaccatccg gcccagcccc
    59941 gttttacccg caggccactg aatatccaca cactgcccca tattttttca gctctgtcac
    60001 cggcgatcgc ccccgttctg gctgatggag acaacaacag actcttccac ggacaaaaac
    60061 gtcccgagcg ccgtgtcgca ctaacccccg aagtctttgt ggagcagaac aaccttatcc
    60121 gcaacatgca gctcgatgtt gcgtccacac gccagtgctt catggcgcaa ctggggcaac
    60181 cactgaccag ctggaaagac atggccccac atgaaaaagc gctctttgcc atctttggcc
    60241 tgcagttctt cctcggtgac cggaaagcag cggtagccct gatgaacaac ctcaatctct
    60301 cctgcaggct caaaagtaaa cgggaccagg ggcgtttcag cacgccggtg tattcactgg
    60361 ccagaaacgc ctttatccgg gtcatcaaaa cagaaggcgc acaacagtgg ctcaggcagc
    60421 accgttacgt tcgcagcggt ctggtctggc tttacgcaca tgatttgcgc ctgacgcctc
    60481 cgaactggct gtggctcaag ggcgttgacc ggacgctctt ctatgccctt caccgggcaa
    60541 acaccacaaa aggcttcatc gaaggggccg gcgtggtggc cgttgcccgg gcagaaaatg
    60601 aagccagccg tctcggcctc ccctgcccgg aaccctgtgt ggaagaggcc attgaagggc
    60661 ttcgacggga catgctgggg ctgggcctga tatgggacga acctcagcca gatcgcgatc
    60721 gtaaacgtca gatcaggacc cgctggtcac tcacggatga cgtgataccc cgccgccatg
    60781 ataacgacga agacactgac actggtgaaa ccaccgaaac caccgaaacc accgaaacca
    60841 ccgaaaccac cgaaaccacc gaaaccaccg aaaccaccga aaccaccgaa accaccgaaa
    60901 aacagcatcc cgccgacaag gaaaaagaac aatgaccatc cactacactg caacactgaa
    60961 taataaacac ctgtccctga tcgccctgta ccctgacggt aatcgggagg tgattgccat
    61021 cctgccagtc acagaactgg gcgcactgat taaccgtaat aacaggctga tgcacctgtc
    61081 cttcagttcc ccttttcgcg aaataaccga aacggtgctt gttgataccg ttgatgcaga
    61141 acgtcccgga catcacatcg gcagaatgct ctcagcgatt gatgcctgcc tggatgccga
    61201 atatgccgga aaaggaaaac atccctcccg gaaaaatggc ttctggcgga cactgatgtg
    61261 tggctttatc gccggaattg ctctgccggc gacagttgca ggttaccact ggatgaaggc
    61321 agagccccct tccacgctgg cttaccctac atctcaggaa tggagagctg aaccagtaac
    61381 cgacacagag acaccacgcc cacatacgga acatcatgtt aacagcatcc cggcccgccc
    61441 ggctcccaca cagcaggaac cttcttctgc ccccgccccg acggcagtga cggaagaaca
    61501 acacgttcag gccgctgagg cccgccgtaa tctggccaca gtacttaaac gcaacgcaga
    61561 ccgggggatg ttcaccatta acctgtccac tggtcatgaa cgcacgctgt acgccttcct
    61621 tgaccccgca tgcatcaact gccgtctgct ggaacccgcg cttaagcaac tggcccggga
    61681 attcagcgtg gtgatttatc cggtctccgt gattggcggg gagtccagta caaagcggac
    61741 tgctccgctg ctgtgtgaac cggactcaca aaaacgggcg accggatggc atcagctgta
    61801 ttccgctgac agtggtatgg cccccaccgg cgatgcaccg gccgcacagg atggacagtg
    61861 tctgaccgcc gcacgtgctg ccatcgatgt caataaccag gctttccgcc agttcggttt
    61921 tggcggaacc ccctgggtgt tatcagatac cggctggcac ctgccgtccg gcatcctgaa
    61981 agacgccact tccgttcgcc tcttcctgaa agcaacagat agcgaggagt atcacaatga
    62041 gccagcacca cgttaatcct gacctcatac accgcacggc ctggggaaac ccgttgtgga
    62101 atgcactgca taacctgaac atcactggtc tgtgtctggc cggcagtatc attacggcgc
    62161 tgatatggcc actggccctt cctgtctgcc tgctgttcac cctggtcacc tccgtgatat
    62221 tcatgctgca acgctggcga tgccccctgc gtatgcccat gacactcagc cttgatgacc
    62281 cctcccagga cagaaaggtc aggagaagcc tcttcagctt ctggcccacc ctgtttcagt
    62341 atgaagcgga tgaaacattc cccgcccggg ggatttttta tgtcggttac cgccgtatca
    62401 acgatatcgg tcgtgagctc tggctgagca tggatgacct cacccggcat gtcatgtttt
    62461 ttgccaccac cggtggcggt aaaacagaaa ccacctttgc ctggctgctc aacccgctct
    62521 gctggggaag aggctttacc tttgttgacg gaaaggcaca gaacgatacc acgaggacaa
    62581 tctggtatct ctcccgacgt tttggcaggg aggatgatat cgaagtcatc aacttcatga
    62641 acggcggaaa atcccgcagt gaaatcattc agagcggcga aaaatcccgc ccacagtcaa
    62701 acacctggaa cccgttcgca ttcagcaccg aagccttcac cgccgaaacc atgcagtcca
    62761 tgctgcccca gaacgttcag ggcggtgaat ggcagtcacg ggcgatagcc atgaacaagg
    62821 ccctggtctt tggcacaaaa ttctggtgcg tccgggaagg taaaaccatg tccctgcaga
    62881 tgttacggga acatatgacg ctggaaggga tggcaaaact ctattgccgt ggccttgatg
    62941 accagtggcc ggaagaagct atcgcaccac tgcgaaacta cctgcaggat gtcccgggat
    63001 tcgatatgtc actggtgaga acgccgtcag cctggactga ggagccccgt aaacagcatg
    63061 cttatctctc cggtcagttc tcggaaacgt tcacaacctt tgccgaaacc ttcggggacg
    63121 tctttgcggc agataccggc gacatcgata ttcgcgacag tattcacagc gaccgcattc
    63181 tgattgtgat gatccctgca ctggacacat cggctcatac cacgtcggca ctcggtcgta
    63241 tgttcgtcac ccagcagagc atgctcctcg cccgtgacct gggctaccgg ctggaaggca
    63301 cagatgcaca gaccctggaa gtcaaaaaat acaaaggcag cttcccctat atctgtatcc
    63361 ttgacgaagt cggtgcctac tacacagacc gcattgctgt ggaggccacc caggtacgtt
    63421 cactggagtt ttccctgata atgacggcgc aggatcagga gcgaattgaa gggcagacct
    63481 ccgccaccaa caccgccaca ctgatgcaga acgccggcac caaattcgcc ggacgcattg
    63541 tcagtgacga caaaacggca agaacggtga aaaatgccgc cggtgaagag gcacgggcca
    63601 gaatgggcag cctgcagcgc cacgatggtg tgatgggtga gtcctgggtg gacggtaacc
    63661 agatcaccat ccagatggaa agcaaaatcg acgtgcagga tttgatcaga ctgaatgctg
    63721 gtgagttctt taccgttttc cagggggatg tggtgccgtc agcctctgtg tatattccgg
    63781 acagcgagaa aagttgcgac agtgatccgg tggtcatcaa ccgctatatt tccgttgaag
    63841 ccccccggct ggagcgtctc cgccgtctcg tccccaggac agtgcagcgc aggctcccca
    63901 caccggagca tgtcagcagc atcatcggtg tactgacagc aaaaccatcc cgtaaacgtc
    63961 gtaaaaaccg tacagagcct taccgtatca ttgatacatt ccagcgccag ttggcaacag
    64021 cgcagacatc actggatctg cttccgcaat atgatacaga tatcgaatcc cgggcaaacg
    64081 aactgtggaa aaaggctgtg cacactatca ataacacaac cagggaagaa cgccgggtat
    64141 gttatatcac actgaatcgt ccggaagagg aacactccgg tcctgaagat ataccttcag
    64201 tgaaggcgat tctgaatact ctgctccccc tggagatgct gttacctgta ccggatctaa
    64261 ctgcatcccc gccccataaa aaaaatgtcg ctcagacacc atcaggtgga aaacaagaga
    64321 gtcgtaaaaa aagattctga taacacctgt atggctgatt ttaataacaa cctttacgcg
    64381 attattaatc tcctccgctc accatagtaa gcggaggatt caggatttca atccatcacc
    64441 agaggtacaa taaatgtgta ttagtagccc ggaaacaaac tcatggtctg ttatttatcg
    64501 taaaaattcc ggagaagata tcaatatcac ttccctgacg tttaaaaata gtcttctggc
    64561 agcccggaca ctgatggtgc ccgaaaacta tatgatatgc atactcagaa acggggagag
    64621 ggtcagacga tgggacagag aaatacttgc aggctcaaac agatggtaca agtgttctcc
    64681 cgataatttc gaaatactgg gtaagctccc cataataaat aaagtcacaa aactgataaa
    64741 atcataatct acggatttta atcagttaag ctaaatcagg atgaacgctt tgattatgaa
    64801 ttatacatga aaacaaatat gggcagcaca aaaaatggtg ccagggttcc ccacgttcgt
    64861 aatagattca cttctatgga tattggtatg aattcccaga tcccactcta ttgtaaacaa
    64921 gacattttta tcttttatat tcaatggctt attttcctgc taattggtaa taccatgaaa
    64981 aataccatgc tcagaaaagg cttaacaata ttttgaaaaa ttgcctactg agcgctgccg
    65041 cacagctcca taggccgctt tcctggcttt gcttccagat gtatgctatt ctgctcctgc
    65101 agctaatgga tcaccgcaaa caggttactc gcctggggat tccctttcga cccgagcatc
    65161 cgtatgagac tcatgctcga t
//
LOCUS       DBKEKI010000004        65009 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.004, whole genome shotgun sequence.
ACCESSION   DBKEKI010000004 DBKEKI010000000
VERSION     DBKEKI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.004"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACUQPX_000241"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACUQPX_000241"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HHV9055047.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACUQPX_000242"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACUQPX_000242"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HHV9055048.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACUQPX_000243"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACUQPX_000243"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HHV9055049.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="ACUQPX_000244"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="ACUQPX_000244"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HHV9055050.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISDAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="ACUQPX_000245"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="ACUQPX_000245"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HHV9055051.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDTLGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCCDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="ACUQPX_000246"
     CDS             7826..9559
                     /locus_tag="ACUQPX_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HHV9055052.1"
                     /translation="MHSTEVQAKPLFSWKTLGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="ACUQPX_000247"
     CDS             9708..10559
                     /locus_tag="ACUQPX_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9055053.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="ACUQPX_000248"
     CDS             complement(10546..10887)
                     /locus_tag="ACUQPX_000248"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HHV9055054.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="ACUQPX_000249"
     CDS             complement(10889..11767)
                     /locus_tag="ACUQPX_000249"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HHV9055055.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="ACUQPX_000250"
     CDS             complement(11733..14030)
                     /locus_tag="ACUQPX_000250"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HHV9055056.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="ACUQPX_000251"
     CDS             complement(14081..14401)
                     /locus_tag="ACUQPX_000251"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HHV9055057.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15480)
                     /locus_tag="ACUQPX_000252"
     CDS             complement(14416..15480)
                     /locus_tag="ACUQPX_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HHV9055058.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAL
                     IAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGALTNSL
                     TQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAIAAVGI
                     CIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVIPSIMV
                     GSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVLKSLAR
                     KNGSSTDEKEDDLDLDFEIN"
     gene            15789..18290
                     /gene="ptsP"
                     /locus_tag="ACUQPX_000253"
     CDS             15789..18290
                     /gene="ptsP"
                     /locus_tag="ACUQPX_000253"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HHV9055059.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSL
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18302..18964
                     /gene="fsa"
                     /locus_tag="ACUQPX_000254"
     CDS             18302..18964
                     /gene="fsa"
                     /locus_tag="ACUQPX_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HHV9055060.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            18975..20077
                     /gene="gldA"
                     /locus_tag="ACUQPX_000255"
                     /pseudo
     CDS             18975..20077
                     /gene="gldA"
                     /locus_tag="ACUQPX_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0019147 - (R)-aminopropanol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019588 - anaerobic glycerol catabolic
                     process [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20454..20969
                     /locus_tag="ACUQPX_000256"
     CDS             20454..20969
                     /locus_tag="ACUQPX_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HHV9055061.1"
                     /translation="MNLAIADGARQQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVV
                     IRALRSQKVDLQKLVHEDMAKNFAEYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTR
                     PISKNAGDYQAGDIVSWRLDNGLAHIGVVSDGFARDGTPLVIHNIGAGAQEEDALFSW
                     RMVGHYRYFVK"
     gene            complement(20996..21892)
                     /gene="yijE"
                     /locus_tag="ACUQPX_000257"
     CDS             complement(20996..21892)
                     /gene="yijE"
                     /locus_tag="ACUQPX_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HHV9055062.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPRAVEGS
                     GIVLALALVSRKKKEAVSVKSI"
     gene            complement(21986..24166)
                     /gene="katG"
                     /locus_tag="ACUQPX_000258"
     CDS             complement(21986..24166)
                     /gene="katG"
                     /locus_tag="ACUQPX_000258"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HHV9055063.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24495..25385)
                     /gene="metF"
                     /locus_tag="ACUQPX_000259"
     CDS             complement(24495..25385)
                     /gene="metF"
                     /locus_tag="ACUQPX_000259"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HHV9055064.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRSEMSYAICHTLGVRPGL"
     gene            25461..25595
                     /locus_tag="ACUQPX_000260"
     CDS             25461..25595
                     /locus_tag="ACUQPX_000260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055065.1"
                     /translation="MKEKPKGVNRKMTNYGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25734..28166)
                     /gene="metL"
                     /locus_tag="ACUQPX_000261"
     CDS             complement(25734..28166)
                     /gene="metL"
                     /locus_tag="ACUQPX_000261"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HHV9055066.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVMTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28169..29329)
                     /gene="metB"
                     /locus_tag="ACUQPX_000262"
     CDS             complement(28169..29329)
                     /gene="metB"
                     /locus_tag="ACUQPX_000262"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HHV9055067.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEHALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29606..29923
                     /gene="metJ"
                     /locus_tag="ACUQPX_000263"
     CDS             29606..29923
                     /gene="metJ"
                     /locus_tag="ACUQPX_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HHV9055068.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30107..30715
                     /locus_tag="ACUQPX_000264"
     CDS             30107..30715
                     /locus_tag="ACUQPX_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HHV9055069.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30776..30988)
                     /gene="rpmE"
                     /locus_tag="ACUQPX_000265"
     CDS             complement(30776..30988)
                     /gene="rpmE"
                     /locus_tag="ACUQPX_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HHV9055070.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31191..33389
                     /gene="priA"
                     /locus_tag="ACUQPX_000266"
     CDS             31191..33389
                     /gene="priA"
                     /locus_tag="ACUQPX_000266"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HHV9055071.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33545..34570
                     /gene="cytR"
                     /locus_tag="ACUQPX_000267"
     CDS             33545..34570
                     /gene="cytR"
                     /locus_tag="ACUQPX_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HHV9055072.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALCRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34662..35621
                     /gene="ftsN"
                     /locus_tag="ACUQPX_000268"
     CDS             34662..35621
                     /gene="ftsN"
                     /locus_tag="ACUQPX_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HHV9055073.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADTPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35714..36244
                     /gene="hslV"
                     /locus_tag="ACUQPX_000269"
     CDS             35714..36244
                     /gene="hslV"
                     /locus_tag="ACUQPX_000269"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HHV9055074.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36254..37585
                     /gene="hslU"
                     /locus_tag="ACUQPX_000270"
     CDS             36254..37585
                     /gene="hslU"
                     /locus_tag="ACUQPX_000270"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HHV9055075.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKMKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37652..38578
                     /gene="menA"
                     /locus_tag="ACUQPX_000271"
     CDS             37652..38578
                     /gene="menA"
                     /locus_tag="ACUQPX_000271"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HHV9055076.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38671..39156
                     /gene="rraA"
                     /locus_tag="ACUQPX_000272"
     CDS             38671..39156
                     /gene="rraA"
                     /locus_tag="ACUQPX_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HHV9055077.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39241..39486)
                     /gene="zapB"
                     /locus_tag="ACUQPX_000273"
     CDS             complement(39241..39486)
                     /gene="zapB"
                     /locus_tag="ACUQPX_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HHV9055078.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39912..40757
                     /gene="glpF"
                     /locus_tag="ACUQPX_000274"
     CDS             39912..40757
                     /gene="glpF"
                     /locus_tag="ACUQPX_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HHV9055079.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            40780..42288
                     /gene="glpK"
                     /locus_tag="ACUQPX_000275"
     CDS             40780..42288
                     /gene="glpK"
                     /locus_tag="ACUQPX_000275"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HHV9055080.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKTVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42518..43528
                     /gene="glpX"
                     /locus_tag="ACUQPX_000276"
     CDS             42518..43528
                     /gene="glpX"
                     /locus_tag="ACUQPX_000276"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HHV9055081.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43625..44371
                     /gene="fpr"
                     /locus_tag="ACUQPX_000277"
     CDS             43625..44371
                     /gene="fpr"
                     /locus_tag="ACUQPX_000277"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HHV9055082.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44376..44804)
                     /gene="uspD"
                     /locus_tag="ACUQPX_000278"
     CDS             complement(44376..44804)
                     /gene="uspD"
                     /locus_tag="ACUQPX_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HHV9055083.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44831..45130)
                     /locus_tag="ACUQPX_000279"
     CDS             complement(44831..45130)
                     /locus_tag="ACUQPX_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HHV9055084.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45342..45782)
                     /locus_tag="ACUQPX_000280"
     CDS             complement(45342..45782)
                     /locus_tag="ACUQPX_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HHV9055085.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            45883..46482
                     /locus_tag="ACUQPX_000281"
     CDS             45883..46482
                     /locus_tag="ACUQPX_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HHV9055086.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46590..47357
                     /gene="tpiA"
                     /locus_tag="ACUQPX_000282"
     CDS             46590..47357
                     /gene="tpiA"
                     /locus_tag="ACUQPX_000282"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HHV9055087.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47412..48167)
                     /gene="cdh"
                     /locus_tag="ACUQPX_000283"
     CDS             complement(47412..48167)
                     /gene="cdh"
                     /locus_tag="ACUQPX_000283"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HHV9055088.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48274..49263)
                     /gene="sbp"
                     /locus_tag="ACUQPX_000284"
     CDS             complement(48274..49263)
                     /gene="sbp"
                     /locus_tag="ACUQPX_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HHV9055089.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWSYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49583..50545)
                     /gene="pfkA"
                     /locus_tag="ACUQPX_000285"
     CDS             complement(49583..50545)
                     /gene="pfkA"
                     /locus_tag="ACUQPX_000285"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HHV9055090.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50726..51628)
                     /gene="fieF"
                     /locus_tag="ACUQPX_000286"
     CDS             complement(50726..51628)
                     /gene="fieF"
                     /locus_tag="ACUQPX_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HHV9055091.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKILAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51778..52278)
                     /gene="cpxP"
                     /locus_tag="ACUQPX_000287"
     CDS             complement(51778..52278)
                     /gene="cpxP"
                     /locus_tag="ACUQPX_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HHV9055092.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHKQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52428..53126
                     /gene="cpxR"
                     /locus_tag="ACUQPX_000288"
     CDS             52428..53126
                     /gene="cpxR"
                     /locus_tag="ACUQPX_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HHV9055093.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53123..54496
                     /gene="cpxA"
                     /locus_tag="ACUQPX_000289"
     CDS             53123..54496
                     /gene="cpxA"
                     /locus_tag="ACUQPX_000289"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HHV9055094.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54602..55276)
                     /gene="yiiM"
                     /locus_tag="ACUQPX_000290"
     CDS             complement(54602..55276)
                     /gene="yiiM"
                     /locus_tag="ACUQPX_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HHV9055095.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIALGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(55425..56408)
                     /gene="kdgT"
                     /locus_tag="ACUQPX_000291"
     CDS             complement(55425..56408)
                     /gene="kdgT"
                     /locus_tag="ACUQPX_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HHV9055096.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVVTAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(56668..57288)
                     /gene="sodA"
                     /locus_tag="ACUQPX_000292"
     CDS             complement(56668..57288)
                     /gene="sodA"
                     /locus_tag="ACUQPX_000292"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HHV9055097.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            57573..58607
                     /gene="rhaT"
                     /locus_tag="ACUQPX_000293"
                     /pseudo
     CDS             57573..58607
                     /gene="rhaT"
                     /locus_tag="ACUQPX_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(58604..59452)
                     /gene="rhaR"
                     /locus_tag="ACUQPX_000294"
     CDS             complement(58604..59452)
                     /gene="rhaR"
                     /locus_tag="ACUQPX_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HHV9055098.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(59526..60362)
                     /gene="rhaS"
                     /locus_tag="ACUQPX_000295"
     CDS             complement(59526..60362)
                     /gene="rhaS"
                     /locus_tag="ACUQPX_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HHV9055099.1"
                     /translation="MTVLHSVDFFPSCNASVAIELRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            60650..62119
                     /gene="rhaB"
                     /locus_tag="ACUQPX_000296"
     CDS             60650..62119
                     /gene="rhaB"
                     /locus_tag="ACUQPX_000296"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HHV9055100.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            62116..63375
                     /gene="rhaA"
                     /locus_tag="ACUQPX_000297"
     CDS             62116..63375
                     /gene="rhaA"
                     /locus_tag="ACUQPX_000297"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HHV9055101.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            63617..64440
                     /gene="rhaD"
                     /locus_tag="ACUQPX_000298"
                     /pseudo
     CDS             63617..64440
                     /gene="rhaD"
                     /locus_tag="ACUQPX_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            64450..64764
                     /gene="rhaM"
                     /locus_tag="ACUQPX_000299"
     CDS             64450..64764
                     /gene="rhaM"
                     /locus_tag="ACUQPX_000299"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HHV9055102.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT        15836 a        17521 c        16646 g        15006 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca acaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgttcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct gacgccatcc gtattcaggg ccagtcggga cactccagcg
     4081 atccagcacg cggagttaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgctgcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg tactgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatacgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acacaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgctgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttt
     7861 tagctggaaa accctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gttgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaaa tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cgctcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacacc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acgcccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccatga tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagctg ttaccaaccc aggcaccgat agcgcacgga gccagcgcag aacgctctgc
    15241 aatggagtaa ccaatgtaag cggcgaggaa aggcaccatc agcgtcaaac ctgcaacgcc
    15301 gatatcaaac agttttttca aattcggatc ggctacggca tccggcactg cgcctttgcc
    15361 atacaacatg acggaaaccg ccagcaaaat accgcccgat accacgaagg gaatcatgtg
    15421 tgaaacgccc gtcattaaat gctgacgggt atttttcagg atctgcacca actcattcat
    15481 aagctgctcc cgggcttgca aaagcccatg tccatatttt tgatggagga aacaataggc
    15541 aatcgcaggc ggagcaaaca gtggataaaa aggccattta ctggactttt gtaatatcag
    15601 tacaaaaatg cgatccgcct cataacttgc gataatccgg aagtatgccg caaaacggcg
    15661 tcttgtgaga cgcgtcgcaa attgacggtt acattacatt tgtccagctt ttggcagatt
    15721 tgtcacatgg caggccgtta ccgtgcgctc ttatcctgta aacagaaaac cgtaccggga
    15781 gaaaggcaat ggccctgatt gtggaattta tttgtgagct acctaacggc gtacatgcgc
    15841 gtccggcaag ccacgttgaa acgctgtgta atactttttc atctcaaatt gagtggcata
    15901 acctgcgtac tgaccgtaaa ggcaacgcca aaagcgctct ggcgctgatt ggcaccgata
    15961 cgctggtggg tgataactgc cagctactga tttccggggc cgacgaacag gaagggcacc
    16021 agcgtttaag ccaatggctg cgcgatgaat tcccccactg cgatgcgccg ctggcggaag
    16081 ttaaatctga cgaactggaa ccactgccgg tttcactgac caatctgaat ccgcaaatta
    16141 tccgcgcccg caccgtgtgc agcggtagtg caggcggcat tctgacgccg atctcttctt
    16201 tagatctcaa tgcgctgagt aatcttcccg cagccaaaga cgttgacgcc gagcaatccg
    16261 cactggaaaa cggcctgacg ctggtactga aaaacattga gtttcgtctg ctggatagcg
    16321 acggtgctac cagcgcgatt ctggaagcgc accgatccct ggctggcgat acttccctgc
    16381 acgaacattt actggcaggc gtcagcgccg gattaagctg cgccgaagcg attgtcgcca
    16441 gtgcgaatca cttttgcgaa gagttcgccc gttccagcag cagatacctg caagaacgtg
    16501 ccctagacgt acgcgacgtc tgcttccagt tactccagca aatctacggt gagcaacgct
    16561 tcccggcacc gggcaaactg acgcagtccg ccatttgtat ggctgatgaa ctgaccccca
    16621 gccagttcct cgaactggat aaaaatcacc tcaaaggatt gttactgaaa agcggcggca
    16681 ccacctcaca tacggtgatc cttgcccgtt cgttcaacat tccgacgctg gttggtgtgg
    16741 atattgatgc cctcactccg tggcagcatc aaacgattta tatcgacggc aacgccgggg
    16801 cgattgtggt tgagccagga gaagccgtag ctcgttatta tcagcaagaa gcccgcgtac
    16861 aggacgccct gcgtgagcaa cagcgtgtct ggctgaccca acaagcccgt accgctgacg
    16921 gtatccgcat tgaaattgcc gctaacatcg ctcactccgt ggaagcgcag gccgcattcg
    16981 gcaatggtgc ggaaggcgtt ggtttgttcc gcactgaaat gctctatatg gatcgcacca
    17041 gtgcaccggg cgaaagcgag ctgtacaaca ttttttgtca ggcgctggag tctgccaacg
    17101 gacgcagcat tattgtgcgc actatggaca ttggcggcga caaacccgtt gattatctaa
    17161 acattcccgc agaggcaaac ccgttcctcg gttatcgcgc cgtgcgtatt tatgaagaat
    17221 acgcgtcgtt atttaccaca caactacgct cgatcctgcg cgcctccgct cacggcagcc
    17281 tgaaaatcat gatcccgatg atctcctcac tggaagagat cttatgggtg aaagaaaaac
    17341 tggcagaagc caaacagcaa ctacgtaacg aacacattcc gtttgatgag aagatccagc
    17401 tcggcattat gctggaagtg ccgtcggtga tgttcatcat cgatcaatgc tgcgaagaga
    17461 ttgatttctt tagtattggt agtaatgacc tgacgcaata tctgctggca gtggatcgcg
    17521 ataacgctaa ggttactcgt cactacaaca gcctgaatcc ggcattcttg cgggcgctcg
    17581 attacgccgt gcaggcggtg catcgccagg gtaaatggat tggtctgtgc ggtgagctgg
    17641 gagcgaaagg ttccgtgctg ccgttgctgg tcggcttagg gctggatgaa ctcagcatga
    17701 gcgcaccatc aattccggcg gcgaaagcgc ggatggcgca acttgatagc cgtgagtgcc
    17761 gcaagttgct caaccaggca atggcctgcc gtacttcgct ggaagtggaa cacctgctgg
    17821 cgcaattccg catgacccaa caggacgcac cgctggtcac cgccgagtgc atcacgctgg
    17881 aaagtgactg gcgcagcaaa gaagaagtgc tcaaaggcat gaccgataac ctgctgctgg
    17941 cgggccgctg ccgctatccg cgtaaactgg aagccgactt atgggcgcgc gaggccgttt
    18001 tctctaccgg tctgggcttt agttttgcca ttccacacag caaatcagaa cacattgagc
    18061 aatccaccat cagcgtggcg cgtctgcaag cgccggtgcg ctggggcgat gatgaagcgc
    18121 aattcatcat tatgttaacc ctgaacaaac acgctgcggg cgatcagcac atgcgcattt
    18181 tctcgcgcct cgctcgccgc atcatgcacg aagaattccg taacgcgctg gttaacgccg
    18241 cctctgccga cgctatcgcc agcctgctgc aacatgaact ggaactgtaa aaggaaacat
    18301 catggaactg tatctggaca ccgctaacgt cgcagaagtc gaacgtctgg cacgcatatt
    18361 cccgattgcc ggggtgacaa ctaacccgag cattatcgct gccagcaagg agtccatctg
    18421 ggaagtgctg ccgcgtctgc aaaaagcgat tggtgatgag ggcattctgt ttgctcagac
    18481 catgagccgc gacgcgcagg ggatggtgga agaagcgaag cgcctgcgcg acgctattcc
    18541 gggtattgtg gtgaaaatcc cggtgacttc cgaaggtctg gcagcaatta aaatactgaa
    18601 aaaagagggt attactacac ttggcaccgc tgtatatagc gccgcagaag ggttattagc
    18661 cgcactggca ggggcaaaat acgttgctcc gtatgttaac cgcgtagatg cccagggcgg
    18721 ggacggcatt cgtacggttc aggagctgca aacgctgtta gaaatgcacg cgccagaaag
    18781 catggtgctg gcagccagct ttaaaacgcc gcgtcaggcg ctggactgtt tactggcagg
    18841 atgtgaatcc atcaccctgc ccttagatgt agcgcaacaa atgctcaaca cccctgcggt
    18901 agagtcagct atagagaagt tcgaacacga ctggaatgcc gcatttggca ctactcatct
    18961 ctaaaggagc aattatggac cgcattattc aatcaccggg taaatacatc cagggcgctg
    19021 atgtgattaa tcgtctgggc gaatacctga agccgctggc agaacgctgg ttagtggtgg
    19081 gtgacaaatt tgttttaggt tttgctcaat ccactgtcga gaaaagcttt aaagatgctg
    19141 gactggtagt agaaattacg ccgtttggcg gtgaatgttc gcaaaatgag atcgaccgtc
    19201 tgcgtggcat cgcggagact gcgcagtgtg gcgcaattct cggtatcggt ggcggaaaaa
    19261 ccctcgatac tgccaaagca ctggcacatt tcatgggtgt tccggtagcg atcgcaccga
    19321 ctatcgcctc taccgatgca ccgtgcagcg cattgtctgt tatctacacc gatgagggtg
    19381 agtttgaccg ctatctgctg ttgccaaata acccgaatat ggtcattgtc gacaccaaaa
    19441 tcgtcgcggg cgcacctgca cgtctgttag cagcgggtat cggcgatgcg ctggcaacct
    19501 ggtttgaagc gcgtgcttgc tctcgtagcg gcgcgaccac catggcgggc ggcaagtgca
    19561 cccaggcggc gctggcactg gctgaactgt gctacaacac cctgctggaa gaaggcgaaa
    19621 aagcgatgct tgccgccgaa cagcatgtag taaccccggc gctggagcgc gtgattgaag
    19681 cgaacaccta tttgagcggc gttggttttg aaagcggcgg ctggcggcgg ctcacgcggt
    19741 gcataacggc ctgaccgcta tcccggacgc gcatcactat tatcacggtg aaaaagtggc
    19801 atttggtacg ctgacgcagc tggtactgga aaacgcgccg gtggaggaaa tcgaaaccgt
    19861 agctgcactt agccatgcgg taggtttgcc aataactctc gctcaactgg atattaaaga
    19921 agatgtcccg gcgaaaatgc ccattgttgc aaaagcatct tgtgcagaag gtgaaaccat
    19981 ccacaacatg cctggcggcg cgacgccaga tcaggtttac gccgcactgc tggtagctga
    20041 ccagtacggt cagcgtttcc tgcaagagtg ggaataacct actccaaact cccggcttgt
    20101 ccgggagttt gaacgcaaaa ttgcctgatg cgctacgctt atcaggccta cgcaatctct
    20161 gcaatatatt gaatttgcgt gcttttgtag gccggataag gcgttcactc cgcatccggc
    20221 actctgtgcc agtaatctga aactcccacg ttgttcagga gttttttatc cgcttctgga
    20281 cattttctcc acagaacaat cgttagcccg gaaggtcgaa tctgtgactc ttattttcac
    20341 ggacgaacca gatgaaagct tcactggcgc tgctcagcct gctaacggca tttaccagcc
    20401 actccttaaa atctcccgcc gttccaccaa ccgtggtaca aatctaggcc aatatcaacc
    20461 ttgccatcgc agacggtgcc agacagcaaa ttggcagcac gctattttac gatccggcgt
    20521 atatgcagct tacctatcct ggcggtgatg ttccgcaaga acgcggtgta tgttccgatg
    20581 tggtgatccg cgcattgcgc agccagaagg tcgatttgca gaaactggtt cacgaagata
    20641 tggcgaagaa ttttgctgag tacccgcaaa aatggaagtt aaagcgcccg gacagcaaca
    20701 tcgatcaccg tcgggtgcct aatctggaaa cctggtttag ccgccacaac aaaacacgcc
    20761 ccatcagtaa aaatgccggt gactatcaag cgggcgatat tgtctcctgg cgattagata
    20821 acgggctggc gcatattggg gtggtgtcag atggcttcgc ccgcgacggt acgccgctgg
    20881 tgatacataa cattggcgca ggcgcgcagg aagaagatgc gctgtttagc tggcggatgg
    20941 tggggcatta tcgttatttt gttaaataag agatcgcccc acatgaagaa aaaattcaga
    21001 tgctttttac actgacagct tcttttttct tacggctcac cagcgccagt gccagcacaa
    21061 taccgctacc ttcaacggcc ctcggattct caccgagcag ccaccaggaa aagagtacgc
    21121 cacaaacggg aacggccagt gtgcttaagc tggcaatact ggcaggcaag tttttcagca
    21181 caaacagcca taagctccac gctagcgccg tcgccagaat cgcactgtag gccagcgccc
    21241 agaacacggt gggctgccag tcaatttcac gttgcggcac cagtaaagcg accacactca
    21301 tcaccagcgc cgcatacagc atctgccagg atgttaacga taataaatcc acgcgcggat
    21361 gacgggcgta taggcgttta gcaacaatcg cgctcgcccc ccaactaaca ccggagagga
    21421 ttgccagcat ggcacttttc atcgaagaga aatcgagttg ccacggctgc aacaccaaaa
    21481 ataagccgaa agcggcaatc agaatcgcga aatattgccc acgtcgcagg cgttcaccga
    21541 gaaacaacgc ggcgaaaatc accacccaga acggcatggt atagctcagg atcgccacct
    21601 tccccgcacc tccgctgacc aacgcccact gcgccagacc aaccatcccg caggtttgta
    21661 acagggcaat ggctaaggtg tatttaaacg gcgtcgggcg cattccgcga ccacgtaata
    21721 aaaggacgat gaataaaacg agagcgccga aaatgcagcg taaggcggta aagtcgaagg
    21781 caccgatgta actggtgact tgcttcatga aaatccagct ataactccag ataagtgtga
    21841 gcacaaccag gccactgatt gccagtgggt tgctctttcc tgcggcagac atgagagatc
    21901 cagtgtgtag ttttcctgtg gacactatac gccggtaaag aaacttcagc gactgcctga
    21961 aagacagtcg ctgaagaagg tcagattaca gcaggtcgaa acggtcgagg ttcatcactt
    22021 tcacccatgc cgctacgaag tctttaacaa acttctcgtg ggcatcgctg ctggcgtaaa
    22081 cttccgctac tgcacgcagg acagagttag aaccaaatac cagatccgca cggctggctg
    22141 tgtatttcac ttcgccggtt tcgcggtcac gtccttcgaa cagctctttc gattcgtcag
    22201 tcgctttcca ctcgtaacgc atatccagca agttcacgaa gaagtcattg ctcaatacgc
    22261 caacgcggtc agtgaagacg ccgtttttgc tgccatcgaa gttggcaccc agtacacgca
    22321 tcccgcccac cagcgcagtc atttccggcg cggtcagcgt cagttgctgt gctttgtcga
    22381 tcagcagtga ctcggtggtg gaaacgtcca gacgagcgcg atagttacgg aaaccgtcag
    22441 caattggctc cagcagctca aacatctcaa tgtcagtctg atcctgacgc gcatcaacgc
    22501 gacccggcgc aaacggtaca tgaatgctca aacctgcggc gcttgcggct ttctcaacac
    22561 caaccacacc agccagcacg atgatatccg ccagcgaggc tttaccagac tctttctgga
    22621 ttttctccag aacaggcaga gcacgaacgg ctgcggcgtt cacatcccag tcgcgctgcg
    22681 gcattaatgc cagacgcgca ccgttggcac caccgcgttt gtcgccacca cggaaggtag
    22741 aagcagatgc ccaggccacc gataccagct cactaacaga cagaccagaa tccgcaatcg
    22801 cgaatttcag atcgataatg tcctgctcgg tcgggttgta gatcggctgc ggcagcggat
    22861 cttgccagat cagatcttct ttcggcactt ccggcccgat gtagcgagat ttcggcccca
    22921 tatccctgtg cgtcagtttg aaccaggcac gggcaaaggc ttcgttgaac gcctgcggat
    22981 cgttgaggaa acgacgagag atcttctcga actcaggatc aaaacgcagc gtcaggtcgg
    23041 tcaccagcat tgtcggttta cgtttcttcg acggatcgaa cggatccggg ataatttccg
    23101 gtgcgtctac cgcttcgaac tggattgctc cagccggact gcgggtctgt acccactcat
    23161 acttgaacag attctcgaag aaatagttgc tccactgggt cggcgtctgg gtccagacta
    23221 cttccagacc agaggtaatg gcatctgcgc caacgccgct gccgtaagtg ctcgcccagc
    23281 ctaaaccttg ttcttcaatc ggtgcagctt ctggatcagg acctacgttt gatgtcggac
    23341 cggcaccgtg ggttttaccc agcgtatgac cacccgcaat cagcgccacg gtttcttcgt
    23401 cgttcatgcc catgttgccg aaggtcgcgc ggatagctgc tgccgcagaa agcggttcgc
    23461 cgctgtgatc cgggccttcc gggttaacgt aaatcaggcc catctcggtt gcacccagcg
    23521 gtgctttcgc cagcgcttcc ggatgacggt gagtcagcca ggctttttca tcaccccagt
    23581 taacatccag atccggttcc cagacgtctt cacgaccggc accaaaaccg aaggtacgga
    23641 agccggagtt ttccagcgcc acgttacctg cgaggataaa caggtcggcc caagagattt
    23701 tctgaccata tttctgtttg attggccaca acaggcgacg cgctttatcg aggcttacgt
    23761 tatccggcca ggagttcagc ggtgcaaaac gttgctgacc acgacccgcg ccgccgcgtc
    23821 catcgattga acggtaagta cctgcgccgt gccaagccat acgaataaac agaccggcgt
    23881 agctgcccca gtcggctggc caccacggct gagattctgt cagcagggct ttcagatctt
    23941 ttttcaggcc gtagtaatct aatttgctga attctttgcg gtagtcaaag tcctcaccca
    24001 gcgggttaga acgattagaa tgttggttta acaggtcaac acggagttga tttggccacc
    24061 agtcgcgagt ggttgtgccc gcccccgcgc tctggtcgtg accgccctga tggaacgggc
    24121 atttgccagt ggctgtggtg ttatggatat cgtctgacgt gctcatcaat gtgctcccct
    24181 ctacagtgtt accgttacga tacacagcgt tagagagaag ttataattga attaatccac
    24241 ggatgcgata gttgagatct tacatattgt tggttaaaga gatgtagatc aaattgatcg
    24301 taattaagct tttttgatta tgtccgaatt cggacaaatg ctttataaaa aagggtttta
    24361 gcttcatatc cttcaagtaa tgagggagaa aacaaggctg acaaccttat tttcccgccc
    24421 tcatttcgag gcagcatttt gtgctctgtt taaaatttgt gatcactgtg tgattttcac
    24481 aaaagccaca ctatttataa accaggtcga acacccagcg tatggcaaat cgcgtaactc
    24541 atttcagagc ggttaagcgt atagaagtgg aaatctttca ctccttcacg gcttaaaatc
    24601 ttcaccatat ccatggcaat attcgcgccc accagtttgc gggtttcggc atcgtcatcc
    24661 agcccgtcaa acatttgcgc catccacgcc ggaatacgca cgttggtcat gtcggcaaat
    24721 ttcttcgcct gtttaaagtt agataccggc aaaatgcccg ggatgatttc cacatcaatg
    24781 cccgccgata cacagcggtc acgaaaacgc aggtagcttt cgacatcgaa gaagaactga
    24841 gtaatcgcgc ggttggctcc ggcatccact ttgcgtttca gattaagcaa atccgcctga
    24901 gcgctttttg cttccgggtg aacttccgga tacgccgcca cggatatatc gaaatctgcc
    24961 acttctttta acagcgtcac caggtcagaa gcatacattt ctggcttacc acttcccggc
    25021 ggcagatcgc cacgcagtgc cacgatatga cgaataccgt tattccagta gtcgcgtgca
    25081 atggtgcgca gctcgtcggg cgtcgcatca atgcaagtaa gatgcggtgc cgcttccaga
    25141 ccagtgcgat ctttaatgcc tttaataatg ctgtgcgtac ggtcgcgctc gccggagttc
    25201 gcgccatagg tcaccgatac aaacttcggt ttcaggctgc taaggcgatc gatggagttc
    25261 cacagggtct gctccatttc actggtacgc ggcgggaaaa actcgaacga aacgttaatc
    25321 tgcccctgga cttctgccag gctctgattc agggcatccc gctggctggc gtgaaaaaag
    25381 ctcatacctt acctcatcaa tcgcttgtag ttatatgttg tgttgtgcac atctatccat
    25441 ttagacgtcc agatgtaaag atgaaggaaa agccgaaggg cgtcaaccga aaaatgacca
    25501 attatggtga ggaaaaatca gggaagatga aaaaacttca ggatggtaag aaaaagaaaa
    25561 tccgatatgc aaagcaaacc ggactgcaaa tataaactaa ccatgtactt attgcttctt
    25621 gccggattcg tcgtaaacgc ttcatccggc ctaactctat gataattcaa tatatttcat
    25681 tagaaaccat agacccgaaa acatgagtac cgggcattat taaatttctg aaattacaac
    25741 aactgtgcca gccggttgat atccgactga atcgccccgg cggtgacgtc gcgcccagcg
    25801 ccaggcccac ggatcaccag cggattatcg cggtaccagc ggctttcgat ggcaaagacg
    25861 ttatcgcatg gcagcagtga tgccagcgga tggtcttcac gcaccgcctc cacgcccaca
    25921 cgcgctttgc cgttggcatc gaaacgcgcc acgtagcgca gcaccagccc catttcgcgg
    25981 gccgcttcca gccgttgcac catctgctcg ttaagttcat cgccattttc aaagaaatgg
    26041 tcgatgctgc cgccttcgca atgagcaggc accagcgatt ccacacgtac ctggtccggt
    26101 tcaatgttgt aacccgcttc acgcgccaga atcaccagct tgcgcattac gtctttgccg
    26161 gagaggtcgt cacgcggatc aggctcggtt aagccctgct gccacgcctg atccaccagc
    26221 tcggtaaacg gcacgctacc gtcgaattgc aggaacagcc aggagagcgt gccggagaag
    26281 atcccactga tcgacaaaat ggtatcgccg ctgtcgatca gatcgcgcac ggtatggttg
    26341 atcggcaagc ccgcaccaac ggtggcgttg tatagccagt gacgcccggt tttttcgaag
    26401 gcgtcgtgga tctggcgata tttattgctg tcgcttgctc ccgccagttt gttggcactg
    26461 atgacgtgga aaccgtggct ggcgaaatcg agatactgat cagcaagctg ctggctggcg
    26521 gtaacgtcca acaccactaa atcatcatac ggatgggcgc gcatccacag gaaaagcgac
    26581 tcttcatcct gctcgaccgc ttcatcgttg aagaaggcta acgcgcggct ggcgtccagc
    26641 ccgtcataac tcaacagact gcggcggctg tccaccacgc ctgccagcac aaattcaaag
    26701 ccggtacgcg ccgaaagcgt gctctgctcg cgggcgaaca gttccagcca gcgagagccg
    26761 atattgcctt tgccgaacaa taccaggccg atgcgttttt ctgcgcggaa aacggactga
    26821 tgcagcccct gaatcaggct ttcggtcggg ccggtgcgca gtactgccac cagactgatg
    26881 ccgtcatcgg actgccacgt aaactcgacc ggctggcctt tcagttgctg ccagaagcgg
    26941 tggcaatgca gcgggttacg ggtgacaccc gccccgacca tcgccaccag cgccagcccc
    27001 tgacgcaggc gcagttctcc aggtaatccc gcttcgtcga ggattttcag cgcactgtcg
    27061 gccacttctg aggtgtagca aaattgcagc aactggcgat cgttatgcac gccaaccgcc
    27121 agtggacgca cctgggcacg tttcaggatc tggtcgatct ctttatgcgc cagtttgaaa
    27181 tcctgactgg cgggcacctg aaactcaatc aaacagacat catcgtggct ggtgacaata
    27241 cgcgcaccag taccggaggc cagcacgcgt tcaatgcgcg tggaaccttg atccggcgtg
    27301 tagctacagc gcagctgcag gtcgatttcg ctgccagaaa ctggctgtaa agtacgggcg
    27361 tgaagaacgg gagccgccag acgcgccagt tcgctggcct catccagacg cagcaacggc
    27421 agcaggcagg catctttcac tttacgcggg tcggcactgt ataccccggc gacgtcgctc
    27481 cagatggtta cacgagaaac acccgccagc gcaccgattt gtgtcgcgga atagtcggaa
    27541 ccgttacgcc ccagcaacac cgtttcaccg gcgttgttac ggctgataaa cccggtcatc
    27601 accagacgtt tgctcggatg ttgtaccagt agctgttgca gcaacgggta agaaagccct
    27661 tcatcaacct gcggttgcgc agcgcgttca gcgcgtaaaa actcacgggc atcaagccag
    27721 gccgctggca gcccttgttg attaagtacc gcagacatca ggcgcgccga ccacacttca
    27781 ccgtggccca ccacttccgc atacaccgca tcgttaatac cactgtcgag caacgccgcc
    27841 aggcgctcaa gatcgctgac aaaagcgcta atgaggctat cggcttcttc agcgggtagc
    27901 agaccgctaa tcagatcgca ctgatagcga cgcagcgttt gttgaacctg atgcgcagag
    27961 agacgatcgg tctggcttag tttcaaccag ttaatcaact ggttagtggt gctaccggcg
    28021 gcggaaacca ccatcatatc gtcaggctga gagaactccg ccataatgcc cgcgacacgc
    28081 aaataacact tcacatcagc cagactactg ccaccaaatt tatgcagctg acgacctttc
    28141 gcccctgcct gcgcaatcac actcattttt accccttgtt tgcagcccgg aagccatttt
    28201 ccaggtcggc aattaaatct tcgccatctt caataccggt ggagatacgc agcagcgtct
    28261 cggagatccc ggcggcagca cgcgcttctg gtgccatgcc tgcatgtgtc atggttgcgg
    28321 cgtgagagat taaactttcc actcccccta atgattccgc cagcgtaaac aacgacagcc
    28381 cgcccaggaa acgacacagc gtctgctcat cgccatccag ttcaaaactc aacattgcgc
    28441 caaaaccttt ttgctgacgc gcggcaattt catgcccctg attttccggc agcgacgggt
    28501 gatacagttt tttcaccaac ggctgggttt gcagatattt cacaatcgcc tgcgcgttgc
    28561 gctgcgccag ctccatacgc ggcaccagcg ttcgcaaccc gcgtagcagc agatagctgt
    28621 caaacgcgcc gcccgtcacg ccaatattgt ttgcccacca ggcaagttca gtgacaacgt
    28681 ccgggtcttt agcaatcacc acgccagcca ccacgtcaga gtgaccgttc agatatttcg
    28741 tacatgaatg caacaccaga tcggcaccca atgccagcgg attttgtaat gccgggctta
    28801 agaaggtgtt atccaccacg ctcaccgccc cgacttccct tgccagatgg cagattttcg
    28861 caatatccac gacgcgtaac aatggattac ttgggctttc taccagtacc agtttgggtt
    28921 tttctgccag cgctgcccgt aatgcatgtt catcgccttg atcaacaaac aacacgcgat
    28981 agcaaccgcg tttcgccaga ctgtcgaaca ggcgatagct accgccgtag cagtcgtgcg
    29041 gcgcaaccag cagatcgcca ggtttcaaaa agacggtcgt taccaggtga atcgcggaca
    29101 tgccggtatt agtaagtact gcgccagcac cgccttccag ttctgccagc gcacgctgaa
    29161 ccacatcgcg cgttgggttg ccgcgacgcg agtaatcatg cgcgcgcggt tcattaaatc
    29221 cggtaaagtt ataggtgctg gaaagatgga tgggagggac aacgcaacca tactgttcgt
    29281 cgtcatttaa cccgctacgc actgcgatgg tggcctgttt acgcgtcatg tgatgaagtt
    29341 tcctgggctt tgtcggtgaa atgtcaggca ccagagtaaa cattgtgtta atggacgtca
    29401 atacatctgg acacctaaac ttctttgcgt atagattgag caaatcccaa atagccgtta
    29461 aaattatatg cattatcacg ccgacaggtg cattacacga tgtcacggta acgcctgtac
    29521 ggtaaactat gcgggtttac ggtcagtacc cacataaatt gtggggtctg gtctcaattt
    29581 attgacgaag aggattaagt atctcatggc tgaatggagc ggcgaatata tcagcccata
    29641 cgctgagcac ggcaagaaga gtgaacaagt caaaaagatt acggtttcca ttcctcttaa
    29701 ggtgttaaaa atcctcaccg atgaacgcac gcgtcgtcag gtgaacaacc tgcgtcacgc
    29761 taccaacagc gagctgctgt gcgaagcgtt tctgcatgcc tttaccgggc aacctttgcc
    29821 agatgatgcc gatctgcgta aagagcgcag cgacgaaatc ccggaagcgg caaaagagat
    29881 catgcgtgag atggggatta acccggagac gtgggaatac taaagcggaa aagagcggcg
    29941 cggagtggaa tcgcctgatg cactacgctt atcaggccta cgtcatattg caatttattg
    30001 aatttgcacg aacttgtagg ccggataagg cgttcacgcc gcatccggca taaacaacga
    30061 gcacgttgtc tgcgacccac cgctttttat acatggacgt ttaactatga aaaacaggct
    30121 gctgatcctc agcctgctgg tttctgtacc tgcctttgcc tggcagccgc aaactggcga
    30181 catcatcttt caaatctctc gctcatcgca aagtaaagcg atccaactgg caacccattc
    30241 cgattacagc cacaccggta tgctggtgat gcgtaacaaa aagccctacg tttttgaagc
    30301 ggtcggcccg gtgaaataca ccccgctcaa gcagtggatt gcccatggtg aaaagggcaa
    30361 ctacgttgtt cgccgcgttg aaggcggact gagtgttgaa caacagcaaa aactgacgca
    30421 aacggcaaaa cgttatctcg gtaaaccgta tgatttcagc ttctcatgga gcgacgatcg
    30481 ccagtactgt tcggaagtgg tgtggaaggt ttaccagaac gcgctgggaa tgcgcgtggg
    30541 cgaacagcag aagttgaaag agtttgatct cagcaacccg ctggtccagg cgaagctcaa
    30601 agaacgctac ggtaaaaata ttccgcttga ggaaacggta gtttcaccac aagccgtttt
    30661 cgacgcgcca caactcacca cggttgccaa agagtggccg ctgttttcgt ggtgatacga
    30721 ccaaaattca ggcacaaaaa aagcgccgtg cggcgctttt ttcggaaatc cggtcttatt
    30781 tgctgcccgg gatgttgaaa cgcttgttga agcggtcaac acggccaccg gtagcaacat
    30841 cacgctgttt gccagtgaag aacgggtggc acttgctgca cacgtcgagg ttcaggtcat
    30901 gaccaacggt ggagcggatt ttcattacgt taccgcaaga gcagctagca gtaatttctt
    30961 cgtatttcgg gtgaatatct tttttcatgg gaaaacctcg gtttaaggcc gcgtcgctct
    31021 tccagcccta acgccagaca ccacgcgatg ttaaaagtat agcttcaata cgatcatttc
    31081 gtacgaaggc gcgaaatcat acagaaatta accagcgtat gcaaactgat ccgcactctt
    31141 ctacggcaat gtgtatacta acccaccgaa tttcaagtca ggatgatgct atgcccgttg
    31201 cccacgttgc cttgcccgtt ccgcttcctc gtacctttga ctatctgctg ccagaaggca
    31261 tgacggttaa agctgggtgt cgcgtgcgcg tgccgtttgg caaacagcag gagcgcatcg
    31321 ggattgtggt atcagttagc gatgccagcg aactgccgct cactgagcta aaagcggtag
    31381 tcgaagtgct ggatagtgag ccggtgttta ctcactccgt ctggcgattg ctgctatggg
    31441 cggcagatta ctatcatcat ccgattggcg atgtgctgtt tcatgccttg ccgattttac
    31501 tacgccaggg gcggcctgcg gcgaacgcgc cgatgtggta ctggtttgcc actgaacaag
    31561 gtcaggcggt ggatctgaac agcctgaaac gctcacccaa gcaacaacag gcgctggcag
    31621 cgttacggca aggcaaaatc tggcgcgacc aggttgccac gctcgaattt aatgatgccg
    31681 cgttgcaggc gctgcgcaaa aaaggtctgt gtgatttagc aagtgaaaca ccagagttta
    31741 gcgactggcg aacgaactat gccgtttctg gtgagcggtt gcgattgaat accgaacagg
    31801 ccaccgccgt tggcgcaatt catagcgcgg cagatacttt ttctgcctgg ctgctggcgg
    31861 gcgttaccgg ttccggtaaa acggaggttt atctcagtgt actggaaaac gtgctcgctc
    31921 agggcaaaca ggcgctggtg atggtgccgg aaatcggcct gacaccgcaa actatcgccc
    31981 gttttcgtga acggtttaat gcgcccgtgg aagttctgca ttccggtctg aacgacagcg
    32041 agcgtctttc ggcgtggctg aaagcgaaaa atggcgaggc ggcgattgtg atcggcaccc
    32101 gctccgcgct gtttacgccg tttaaaaatc tcggcgtgat tgtcattgac gaagagcacg
    32161 acagttccta caagcagcag gaaggctggc gctatcatgc ccgcgacctg gcggtgtatc
    32221 gtgcgcatag cgagcaaatc ccgattattc ttggctccgc aacgcccgcg ctggaaacgt
    32281 tgtgcaacgt ccagcagaaa aaataccgcc tgctgcgcct gacgcgccgg gcggggaatg
    32341 cgcgtccggc aattcaacac gtgctggatt taaaaggtca gaaggtgcag gcaggcctgg
    32401 ctccggcgtt aatcacccgt atgcgccagc atttacaggc caacaatcag gttatcctct
    32461 ttcttaaccg ccgtggcttt gcgcctgcgc tactgtgcca cgactgcggc tggattgccg
    32521 aatgcccacg ttgcgatcac tactacacgc tgcatcaggc gcaacagcat ttgcgctgtc
    32581 accactgtga cagtcagcgt ccggtgccgc gccagtgccc ttcctgcggt tccacgcacc
    32641 tggtacccgt ggggctgggc accgaacagc ttgaacagac gcttgcgccg ctgttccctg
    32701 atgtgcccat ttctcgtatc gaccgcgata ccaccagccg caaaggggcg ctggaacagc
    32761 aactggcgga agtacatcgc ggcggtgcga ggattttgat cggcacacaa atgctggcga
    32821 aaggtcacca ctttccggat gtgacgctgg tggcgttact ggatgtggac ggcgcgctgt
    32881 tttctgccga ttttcgctcg gcagagcgtt tcgctcagct ttacacccag gtcgctggtc
    32941 gtgccgggcg tgcgggtaaa caaggcgaag tggtgctgca aacgcaccat ccggaacatc
    33001 ctctgttgca aacgttgctc tataaaggct acgacgcctt tgccgaacag gcgctggctg
    33061 agcgacggat gatgcaactg ccgccatgga ccagccatgt gattgtgcgt gcggaagatc
    33121 ataacaatca gcatgcgccg ctgttcctgc aacaactgcg taatctgatc ctctccagcc
    33181 cgctggcaga cgacaaactg tgggttctcg gtccggttcc ggcactggca cctaaacgtg
    33241 gcggtcgctg gcgctggcag atattgttgc agcacccttc ccgcgtgcgc ttgcaacaca
    33301 tcattagtgg tacgctggcg ctcatcaata caataccgga ttcccgcaag gtgaaatggg
    33361 tactggatgt tgatccgatt gagggttaaa ccgcttacga tgcgaggcgg atcgaaaaat
    33421 tcaatattca tcacactttt catgaaaatt ctgtaaccgt tttcacgcgc tatctgctaa
    33481 aaatgttgcc gatgtgaagt aaacatggat gtagtacgcc tgacgtgcca ggcgaggagt
    33541 gagtgtgaaa gcgaagaagc aggaaactgc cgcgaccatg aaagacgttg ccctcaaggc
    33601 aaaagtctct acagcgactg tctcccgagc attaatgaat cccgataaag tctcccaagc
    33661 cacccgtaat cgggttgaaa aagcggcccg ggaagtgggt tatttaccgc agcctatggg
    33721 gcgcaacgtc aagcgtaatg aatcccgcac cattctggtg attgtcccgg atatctgcga
    33781 tcccttcttt agcgaaatta ttcgcggtat cgaagttacg gcggcaaatc acggatatct
    33841 ggtgctgatt ggcgactgtg cgcatcaaaa tcagcaggaa aaaaccttta tcgatttgat
    33901 catcaccaag caaattgatg gcatgttgct gctgggttca aggctgccgt ttgatgccag
    33961 cattgaggaa cagcgtaatc tgccgccgat ggtgatggcg aacgaatttg caccggaact
    34021 ggagctgcct accgttcata tcgacaatct gaccgccgca tttgatgcag taaattattt
    34081 atatgagcaa gggcataaac ggattggctg tatagccggt cccgaagaga tgccactgtg
    34141 tcactaccgc ctgcaaggct atgttcaggc gctgtgtcgc tgcggaatta tggttgatcc
    34201 acaatacatc gcccgtggcg acttcacctt cgaagccgga agcaaagcaa tgcaacagct
    34261 gcttgatctt ccacaaccgc ctactgcagt cttctgccat agcgatgtga tggcgctcgg
    34321 cgcactttct caggcaaaac gccaggggct gaaagtcccg gaagaccttt ccataatcgg
    34381 ttttgataac atcgacctga cgcaattttg tgatccgccg ctgacaacca tcgcgcagcc
    34441 gcgttacgaa atcggtcggg aagctatgct gttattgctt gatcaaatgc aggggcaaca
    34501 cgttggcagt ggctctcgtt taatggactg cgaacttatc atccggggat caacacgcgc
    34561 gttaccttaa agtaaacctt taagactgcc gtgactggtc aaaggcccgc cgcttaagta
    34621 acatggcggg ctgacgaacg aataaataca gcgaaacgat agtggcacaa cgagattatg
    34681 tacgccgcag ccaaccggca ccttcgcggc gaaaaaagag cacctcacgg aaaaagcaac
    34741 gaaatctgcc tgcggtttct cccgctatgg tcgctattgc tgccgccgtt cttgtgacct
    34801 ttatcggagg tctgtacttc attacgcatc acaagaaaga agagtccgag acgctgcaaa
    34861 gccagaaagt gaccggaaac ggactaccac caaaaccaga agaacgctgg cgctacatta
    34921 aagagctgga aagtcgccag ccgggtgtgc gtgcgcccac agaaccttct gccggtggtg
    34981 aagtgaaaac gccggagcaa ctgacaccag aacaacgtca gcttcttgaa caaatgcaag
    35041 ctgatatgcg ccagcagcca acgcagctgg ttgaggttcc gtggaatgaa cagacgccgg
    35101 aacagcgtca gcaaacgcta caacgccagc gtcaggcgca gcaattagcg gagcagcaac
    35161 gcctggctca gcagtcgcgt acgactgaac aaagctggca gcagcagacg cgtacgtcgc
    35221 aagccgcgcc ggtacaggcg caaccgcgcc agtctaaacc agcttctact cagcagccgt
    35281 accaggatct gctgcaaacc cctgcgcaca cgaccgcgca gtcgaagccg cagcaagctg
    35341 cgccggttgc tcgtgccgct gacacgccaa aaccgacggc ggagaaaaaa gacgaacgcc
    35401 gctggatggt gcagtgcggt tcgttcagag gcgcggaaca ggcagagacg gtacgtgctc
    35461 agctggcgtt cgaaggattt gactcgaaaa tcaccaccaa caatggctgg aatcgtgtgg
    35521 tcattggccc ggtgaaaggc aaagagaacg cagacagcac cctcaatcgg ttgaagatgg
    35581 cgggtcatac aaactgcatt cggctcgccg ccgggggttg aaaccctcaa aatccccccc
    35641 atctataatt gcattatgcc ccgtactttt gtacggggtt tgtactctgt attcgtaacc
    35701 aaggggtcag ctcgtgacaa ctatagtaag cgtacgccgt aacggccatg tggtcatcgc
    35761 tggtgatggt caggccacgt tgggcaatac cgtaatgaaa ggcaacgtga aaaaggtccg
    35821 ccgtctgtac aacgacaaag tcatcgcggg ctttgcgggc ggtactgcgg atgcttttac
    35881 gctgttcgaa ctgtttgaac gtaaactgga aatgcatcag ggccatctgg ttaaagccgc
    35941 cgttgagctg gcaaaagact ggcgtaccga tcgcatgctg cgcaaacttg aagcactgct
    36001 ggcagtcgcg gatgaaactg catcgcttat catcaccggt aacggtgacg tggtgcagcc
    36061 agaaaacgat cttattgcta tcggctccgg cggcccttac gcccaggctg cggcgcgcgc
    36121 gctgttagaa aacactgaac ttagcgcccg tgaaattgct gaaaaggcgt tggatattgc
    36181 aggcgacatt tgcatctata ccaaccattt ccacaccatc gaagaattaa gctacaaagc
    36241 gtaaggatct cccatgtctg aaatgacccc acgcgaaatc gtcagcgaac tggataagca
    36301 catcatcggc caggacaacg ccaagcgttc tgtggcgatt gctctgcgta accgctggcg
    36361 tcgcatgcag ctcaacgaag agctgcgcca tgaagtgacc ccgaaaaata tcctgatgat
    36421 cggcccgacc ggtgtcggta aaactgaaat cgcccgtcgt ctggctaagc tggcgaatgc
    36481 gccgttcatc aaagttgaag cgaccaaatt taccgaagtg ggctacgtcg gtaaggaagt
    36541 tgattctatt attcgcgatc tgaccgatgc cgccgtgaaa atggtacgcg tccaggctat
    36601 cgagaaaaac cgttatcgcg ctgaagagct ggcagaagaa cgtattctcg acgtgctgat
    36661 cccacctgct aaaaacaact ggggacagac tgaacagcag caggaacctt ccgctgctcg
    36721 tcaggcattc cgcaaaaaac tgcgtgaagg ccagcttgat gacaaagaaa tcgagatcga
    36781 tcttgccgca gcaccgatgg gcgttgaaat tatggctcct ccgggcatgg aagagatgac
    36841 cagccagctg cagtccatgt tccagaacct gggcggccag aagcaaaaag cgcgtaagat
    36901 gaaaatcaaa gacgccatga agctgctgat tgaagaagaa gcggcgaaac tggtgaaccc
    36961 ggaagagctg aagcaagacg ctatcgacgc tgttgagcag cacgggatcg tgtttatcga
    37021 cgaaatcgac aaaatctgta agcgcggcga gtcttccggt ccggatgttt ctcgtgaagg
    37081 cgttcagcgt gacctgctgc cgctggtaga aggttgcacc gtttccacca aacacgggat
    37141 ggtcaaaact gaccacattc tgtttatcgc ttctggcgcg ttccagattg cgaaaccgtc
    37201 tgacctgatc ccggaactgc aaggtcggct gccaatccgc gttgaactgc aggcactgac
    37261 caccagcgac ttcgagcgta ttctgaccga gccgaatgcc tctatcaccg tgcagtacaa
    37321 agcactgatg gcgactgaag gcgtaaatat cgagtttacc gactccggta ttaaacgcat
    37381 cgcggaagcg gcatggcagg tgaacgaatc taccgaaaac atcggtgctc gtcgtttaca
    37441 cactgttctg gagcgtttaa tggaagagat ttcctacgac gccagcgatt taagcggtca
    37501 aaatatcact attgacgcag attatgtgag caaacatctg gatgcgttgg tggcagatga
    37561 agatctgagc cgttttatcc tataatcgcg ttcaatcatt ttcatcattg tttgatgggg
    37621 ctgaaaggcc ccatttttat tggcgcgtat tatgactgaa caacaaatta gccgaactca
    37681 ggcgtggctg gaaagtttac gacctaaaac cctccccctc gcctttgctg caattatcgt
    37741 cgggacggcg ctggcatggt ggcaaggtca cttcgatccg ctggtcgccc tgctggcact
    37801 aattaccgcc gggctattac agatcctttc taacctcgcc aatgattacg gcgatgcggt
    37861 aaaaggcagc gataaacctg accgcattgg gccgctacgc ggcatgcaaa aaggggtcat
    37921 tacccagcaa aagatgaaac gggcgctcat tattaccgtc gtgctcatct gtctctccgg
    37981 gctggcactg gttgcagtgg catgccatac gctggccgat tttgtcggtt tcctgattct
    38041 tggcgggttg tcgatcattg ccgctatcac ctacaccgtg ggcaatcgtc cttatggtta
    38101 tatcggtctg ggtgatattt ccgtactggt tttctttggc tggttgagtg tcatggggag
    38161 ctggtattta caggctcata cattgattcc ggcactgatc cttccggcga ccgcatgcgg
    38221 cctgctggca acggcagtac tgaatattaa taacctgcgt gatatcaata gcgaccgcga
    38281 aaatggcaaa aacacgctgg tggtgcgctt aggtgaagtg aacgcgcgtc gttatcatgc
    38341 ctgcctgctg atgggctcgc tggtgtgtct ggcgctgttt aatctctttt cgctgcatag
    38401 cctgtggggc tggctgttcc tgctggcggc accattactg gtgaagcaag cccgttatgt
    38461 gatgcgggaa atggacccgg tggcgatgcg accaatgctg gaacgtactg tcaagggagc
    38521 gttactgact aacctgctgt ttgttttagg gatattccta agccagtggg cagcataact
    38581 gacaaatatc aattaacaat tgatgatttt gccaacagcc cacatagcgc gatatactga
    38641 aaattctcgc agtaactgaa tgttaagcct atgaaatacg atacttccga gctttgtgac
    38701 atctatcaag aagatgttaa cgtcgtggaa ccgctgttct ccaactttgg cggacgggcg
    38761 tcgtttggcg gacaaataat cacggtaaaa tgtttcgagg acaacgggtt gctgtacgat
    38821 ctgctcgaac agaatggccg tggtcgtgtt cttgtcgtcg atggcggtgg ttctgttcgt
    38881 cgcgcactgg tcgatgctga actggcgcgt ctggcagtac aaaatgaatg ggaaggtctg
    38941 gtcatttacg gcgcggtgcg tcaggtagat gacctggaag agttggatat cggcatccag
    39001 gcgatggcgg caattccggt tggtgccgct ggcgaaggca ttggcgaaag cgatgtccgc
    39061 gtcaattttg gcggtgtcac cttcttctcc ggcgaccatc tttatgccga caataccggg
    39121 attattcttt cagaagatcc gctggatatt gaatgataag aaaggcaccg caaggtgcct
    39181 tttttctgcg ttacctgttg gcctacacag taaagaaatt acgcgaaaga tgaagcgtaa
    39241 tcagacctct tccatgcgac ccagcagggc ctgcagacgt tcctgccagc cgttctgctg
    39301 ttctttcaga tggttgttct cacgctccag ctcttcgcgc tgatgctggg cattttgaac
    39361 ttcctgcgac aatgagttgt ttttttcttt cagctcttcg atttccatct gcaacagagt
    39421 gatggtatca atcgcctgct gtacttttgc ttccagtttc tcaaacactt ctaatgacat
    39481 tgtcatacct ctcctgaatt gcaaggcgtt gatggataaa aaatcctcgt cccgattacc
    39541 ggtgacgcct taataaatac gagcgcactt tagttagctc cgattgtatg aagccgcgcc
    39601 atcgctgtcc agcggcacgc cttgcagatt acggtttgcc acacttttca tccttctcct
    39661 agtgacataa tccacatcaa tcgaaaatgt taataaattt gttgcgcgaa tgatctaaca
    39721 aacatgcatc atgtacaatc agatggaata aatggcgcga taacgctcat tttatgacga
    39781 agcacacaca ttttaagttc gatatttctc gtttttgctc gttaacgata agttaacagc
    39841 atgcctacaa gcatcgtgga ggtccgtgac tttcacgcat acaacaaaca ttaactcttc
    39901 aggatccgat tatgagtcaa acatcaacct tgaaaggcca gtgcattgct gaattcctcg
    39961 gtaccgggtt gttgattttt ttcggtgtgg gttgcgttgc agcactaaaa gtcgctggtg
    40021 cgtcttttgg tcagtgggaa atcagtgtca tttggggact gggggtggca atggccatct
    40081 acctgaccgc aggggtttcc ggcgcgcatc ttaatcccgc tgttaccatt gcattgtggc
    40141 tgtttgcctg tttcgacaag cgcaaagtta ttccttttat cgtttcacaa gttgccggcg
    40201 ctttctgcgc tgcggcttta gtttacgggc tttactacaa tttatttttc gacttcgagc
    40261 agactcatca cattgttcgc ggcagcgttg aaagtgttga tctggctggc actttctcta
    40321 cttaccctaa tcctcatatc aattttgtgc aggctttcgc agttgagatg gtgattaccg
    40381 ctattctgat ggggctgatc ctggcgttaa cggacgatgg caacggtgta ccacgcggcc
    40441 ctttggctcc cttgctgatt ggtctactga ttgcggtcat tggcgcatct atgggcccat
    40501 tgacaggttt tgccatgaac ccagcgcgtg acttcggtcc gaaagtcttt gcctggctgg
    40561 cgggctgggg caatgtcgcc tttaccggcg gcagagacat tccttacttc ctggtgccgc
    40621 ttttcggccc tatcgttggc gcgattgtag gtgcatttgc ctaccgcaaa ctgattggtc
    40681 gccatttgcc ttgcgatatc tgtgttgtgg aagaaaagga aaccacaact ccttcagaac
    40741 aaaaagcttc gctgtaatat gactacggga caattaaaca tgactgaaaa aaaatatatc
    40801 gttgcgctcg accagggcac caccagctcc cgcgcggtcg taatggatca cgatgccaat
    40861 atcattagcg tgtcgcagcg cgaatttgag caaatctacc caaaaccagg ttgggtagaa
    40921 cacgacccaa tggaaatctg ggccacccaa agctccacgc tggtagaagt gctggcgaaa
    40981 gccgatatca gttccgatca aattgcagct atcggtatta cgaaccagcg tgaaaccact
    41041 attgtctggg aaaaagaaac cggcaagcct atctataacg ccattgtctg gcagtgccgt
    41101 cgtaccgcag aaatctgcga gcatttaaaa cgtgacggtt tagaagatta tatccgcagc
    41161 aataccggtc tggtgattga cccgtacttt tctggcacca aagtgaagtg gatcctcgac
    41221 catgtggaag gctctcgcga gcgtgcacgt cgtggtgaat tgctgtttgg tacggttgat
    41281 acgtggctta tctggaaaat gactcagggc cgtgtccatg tgaccgatta caccaacgcc
    41341 tctcgtacca tgttgttcaa catccatacc ctggactggg acgacaaaat gctggaagtg
    41401 ctggatattc cgcgcgagat gctgccagaa gtgcgtcgtt cttccgaagt atacggtcag
    41461 actaacattg gcggcaaagg cggcacgcgt attccaatct ccgggatcgc cggtgaccag
    41521 caggccgcgc tgtttggtca gttgtgcgtg aaagaaggga tggcgaagaa cacctatggc
    41581 actggctgct ttatgctgat gaacactggc gagaaaacgg tgaaatcaga aaacggcctg
    41641 ctgaccacca tcgcctgcgg cccgactggc gaagtgaact atgcgttgga aggtgcggtg
    41701 tttatggcag gcgcatccat tcagtggctg cgcgatgaga tgaagttgat taacgacgcc
    41761 tacgattccg aatatttcgc caccaaagtg caaaacacca atggtgtgta tgtggttccg
    41821 gcatttaccg ggctgggtgc gccgtactgg gacccgtatg cgcgcggggc gattttcggt
    41881 ctgactcgtg gggtgaacgc taaccacatt atacgcgcga cgctggagtc tattgcttat
    41941 cagacgcgtg acgtgctgga agcgatgcag gccgactctg gtatccgtct gcacgccctg
    42001 cgcgtggatg gtggcgcagt agcaaacaat ttcctgatgc agttccagtc cgatattctc
    42061 ggcacccgcg ttgagcgccc ggaagtgcgc gaagtcaccg cattgggtgc ggcctatctc
    42121 gcaggcctgg cggttggctt ctggcagaac ctcgacgagc tgcaagagaa agcggtgatt
    42181 gagcgcgagt tccgtccagg catcgaaacc actgagcgta attaccgtta cgcaggctgg
    42241 aaaaaagcgg tcaaacgcgc gatggcgtgg gaagaacacg acgagtaatg tcaggcacct
    42301 gaatcgattg cctgatgctg atcgcctgat gcgacgctta cgcgtcttat caggcctacg
    42361 agccaatgcc attttgtgta ggcgggataa ggcgttcacg ccgcatccgg catcacgcag
    42421 actctgaaac cgtgcggggg caaccccgca cacatcaata atcccaccct tcccctgtgc
    42481 tacacttcgc gccattcctt actgcttaga gtttgctatg agacgagaac ttgccatcga
    42541 attttcccgc gtcaccgaat ctgcggcgct ggctggctac aaatggttag gacgcggcga
    42601 taaaaacacc gcggacggcg cggcggtaaa cgccatgcgt attatgctca accaggtcaa
    42661 cattgacggc accatcgtca ttggtgaagg tgaaatcgac gaagcaccga tgctctacat
    42721 tggtgaaaaa gtcggtactg gtcgcggcga cgcggtagat attgctgttg atccgattga
    42781 aggcacgcgc atgacggcga tgggccaggc taacgcgctg gcggtgctgg cagtaggcga
    42841 taaaggctgc ttcctcaatg cgccggatat gtatatggag aagctgattg tcgggccggg
    42901 agccaaaggc accattgatc tgaacctgcc gctggcggat aacctgcgca atgtagcggc
    42961 ggcgctcggc aaaccgttga gcgaactgac ggtaacgatt ctggctaaac cacgccacga
    43021 tgccgttatc gctgaaatgc agcaactcgg cgtacgcgta tttgctattc cggacggcga
    43081 cgttgcggcc tcaattctca cctgtatgcc agacagcgaa gttgacgtgc tgtacggtat
    43141 tggtggcgcg ccggaaggcg tggtttctgc ggcggtgatc cgcgcattag atggcgacat
    43201 gaacggtcgt ctgctggcgc gtcatgacgt caaaggcgac aacgaagaga atcgtcgcat
    43261 tggcgagcag gagctggcac gctgcaaagc gatgggcatc gaagccggta aagtattgcg
    43321 tctgggcgat atggcgcgca gcgataacgt catcttctct gccaccggta ttaccaaagg
    43381 cgatctgctg gaaggtatta gccgcaaagg caatatcgcg actaccgaaa cgctgctgat
    43441 ccgcggcaag tcacgcacca ttcgccgcat tcagtccatc cactatctgg atcgcaaaga
    43501 cccggaaatg caggtgcaca ttctctgagt gatttgatcg attgagcctt ccagtccttc
    43561 gggactggaa tttttttgtt cggagaacga agataaggca agtcaatcaa aacaggagaa
    43621 aaacatggct gattgggtaa caggcaaagt cactaaagtg cagaactgga ccgacgccct
    43681 gtttagtctc accgttcacg cccccgtgct tccgtttacc gccgggcaat ttaccaagct
    43741 tggccttgaa atcgacggcg aacgcgtcca gcgcgcctac tcctatgtta actcgcccga
    43801 taatcccgat ctggagtttt acctagtcac tgtccccgat ggcaaattaa gcccacgatt
    43861 ggcggcactg aaaccgggcg atgaagtgca ggtggttagc gaagcagctg gcttctttgt
    43921 tctggatgaa gtaccggact gcgaaacgct atggatgctg gcaaccggta cagcgattgg
    43981 cccttattta tcgattctgc aactaggcaa agatttagat cgcttcaaaa atctggtcct
    44041 ggttcacgcc gcacgttatg ccgccgactt aagctatttg ccactgatgc aagaactgga
    44101 aaaacgctac gaaggcaaac tgcgcattca gacggtggtc agtcgggaaa cggcagcggg
    44161 gtcgctcacg ggacggatac cggcattaat tgaaagtggg gaactggaaa gcgcgattgg
    44221 cctgccgatg aataaagaaa ccagccatgt gatgctgtgc ggcaatccac agatggtgcg
    44281 cgatacacaa cagttgctga aagagacccg gcagatgacg aaacatttac gtcgccgacc
    44341 gggccatatg acagcggagc attactggta agcggttact tatcgataaa cggcacgatg
    44401 agcaaatccg cactcatctt attgatcatc ccgcgatatg ccggcatcaa acggttgata
    44461 aatgagtgat gatgaccaca gacaaggagg tcgcactgct ctttttgcat aatttccagc
    44521 agtgtttccg gcatttctcc gcgttcaata cgcagttttg tcttcggcca ttgaatattt
    44581 ttcgtcagtt tatacagctt gttatccgac ttattcttca acaattgaag aatatcttct
    44641 gttgcaggga agtagatacc cggatacaac tcgcttaagc catcatcaat atgaattaac
    44701 gtcaggtgag cgtcattatg tctggcgagc tccagggctt tattcaccag taaggcatct
    44761 tcttcattcc cggaaattgc cacgccaatg tgtttataag ccatgtttaa ctccttctaa
    44821 agcaactcca tcaagctaac gaacgcagtg ataactcaaa aataatcatc tcggcctggc
    44881 atgagaaagt gaaggccgca tcaagctcaa cttgcccctc cagatcctta atcttatagg
    44941 ccacgctggc cccttcatct gcagaggatg cagcggcggc aagctctttc agtttggtca
    45001 taaaagactc tgcttcttcg cgggtggcaa aaaagcgcga aaacttactg gtacagttgt
    45061 cgttatcaat caccgtaccg atatcaatcg cacatccttt agtactgcat ttatctacga
    45121 catctttcat agggcacctc tgtatattcg cctctctgtc gattacgact taagaataat
    45181 gcgccgttaa gggaagaata aacaagtaat gatcttatgg attaccattt tttttgatat
    45241 tgcttaaggt gaagagttca gaaggatttt ccgggtattg tcatttgcat cgtggaaata
    45301 acaatcccgg aaaaaaatag cagtaagaga tatattaaag gttaattact tgatttattg
    45361 tcggctttat acttcacatc ctgagtatct ttaccatatt tattttcgcc ttgtgtgcca
    45421 acaaacgcac caagatcgat aagcatcatc accagaatca acgtcgggac aaaacgcccc
    45481 accgcccatt gccagacacc cggtaaaatc gcccagttac ccgccagcag catccacgcc
    45541 acaatcatca gaaatgccca tgcgccggaa cgcccgcgat catgcaagcg cttaacagtt
    45601 actgccgctg ttggccagag caagcacaca aggcaaaacg ccgcggtctg aatatcgagt
    45661 agattcttac ccgccagtga gaaaagcacc agcatgcctg cgaaccacag gcctatccaa
    45721 atccagaaat cacggcgtcc aatacgccct ttaaatgaga ataaccattg ctgtatggtc
    45781 atgtaagttc cttgatggtt gtcttttcct ggattctacc cttttgacaa gggcgacaga
    45841 tatcgtttta atcggagcca gtcataacaa aaggtactgt caatgaagcc agggtgtacg
    45901 ctgttttttc tcttatgttc tgcattaacc gttacaacaa cggcgcatgc acaaacacca
    45961 gatacggcaa cgaccgcgcc ttatctgctg gctggagccc ctactttcga tctctccatc
    46021 agccagtttc gagaagactt taacagccag aatcccagcc tgccactgaa cgaatttcgt
    46081 gccatcgaca gcagtcccga caaagccaat ctcactcgtg ctgcaagtaa aattaacgag
    46141 aacttgtatg cttctacagc gctggagcgc ggtaccttaa aaatcaaaag cattcaaatg
    46201 acctggctac ccatccaggg gccagagcaa aaagcagcga aagcgaaagc tcaggaatac
    46261 atggcagcag tgatccgcac actcacccca ttaatgacca aaacacaaag ccagaaaaaa
    46321 ctgcagtcgc tactaacggc ggggaaaaac aaacgttatt acaccgagac agaaggtgca
    46381 ctgcgttatg ttgtcgcgga caacggcgaa aaggggctga ccttcgctgt tgaaccgatt
    46441 aagctggcgc tatctgaatc gcttgaaggt ttgaataaat gacaaaaagc aaagcctttg
    46501 tgccgatgaa tctctatact gcttcacaga cctgctgccc tgcggggcag ccatcttcct
    46561 ttattcgctt ataagcgtgg agaattaaaa tgcgacatcc tttagtgatg ggtaactgga
    46621 aactgaacgg cagccgccac atggttcacg agctggtttc taacctgcgt aaagagctgg
    46681 caggtgttgc tggctgtgcg gttgcaatcg caccaccgga aatgtacatc gatatggcga
    46741 agcgcgaagc tgaaggcagc cacatcatgc tgggtgcgca aaacgtggac ctgaacctgt
    46801 ccggcgcatt caccggtgaa acttctgccg ctatgctgaa agacatcggc gctcagtaca
    46861 tcatcatcgg ccactctgaa cgtcgtactt accacaaaga gtctgacgaa ctgattgcga
    46921 aaaaattcgc ggtgctgaaa gagcagggtc tgactccggt tctgtgcatc ggtgaaaccg
    46981 aagctgaaaa cgaagcggga aaaactgaag aagtttgcgc acgtcagatc gacgcggtac
    47041 tgaaaactca gggtgctgcg gcattcgaag gtgcggttat cgcttacgaa cctgtatggg
    47101 caatcggtac tggcaaatct gcaactccgg ctcaggcaca ggctgttcac aaattcatcc
    47161 gtgaccacat cgctaaagtt gacgctaaca tcgctgaaca agtgatcatt cagtacggcg
    47221 gctctgtaaa cgcgtctaac gctgcagaac tgtttgctca gccagacatc gacggcgcgc
    47281 tggttggcgg tgcttctctg aaagctgacg ctttcgcagt gatcgttaaa gctgcagaag
    47341 cggctaaaca ggcttaagtc tgacaggtgc cggatttcat atccggcact tactttcctt
    47401 aactcctcgc cttaacgcaa aatctcacac tcatgatcct ggatttcctc ggctgaagca
    47461 cggttcagcg tcagtagatt tcgttgtgtc gccagcaaca caaacgaatt atcactctgc
    47521 cgtaccatcg ccagcccgta gcttcccata tgttcccgcg cctcaggcac ttcttctgca
    47581 agcatcataa atgggctgcg ttgtaccagt tcgctttccg ttacccgacg cgccaggtat
    47641 tcatgcccgc gcaaaccacc tggcagtggc aaccagcggc tgctgatgtt cgccagattt
    47701 ttatccagtt gttcgcgcac atcaggacga atacaagaga tatgaatatg aaaatggttt
    47761 tgcgtacgcc cggtgcggga gttgatcgcc aaagaaaccg cgcgatcggg aaccggctgg
    47821 ccgtattttt tgctcataaa atcacgcgcc tgccaggcca accaaaagaa gttcggcgtt
    47881 gaaggatcgg tcaacaaagg actttcagta ccgttaatac gatacgttgg catcaacaga
    47941 tattgcagtg gaccattaag atcttttaaa accacgtatc cggcattggg tttgacttcc
    48001 gcacatggcg aaggattttg attttgctgc tgattgggca aacattcctc aaggacaatc
    48061 ttacgtaatg tatccgactc ttcaccggtt aatttccagt aaccaatacc ggcagccaca
    48121 acggcgataa ctatcatcac caaaaaaaga agacccgctt ttttcatctt tttttccctg
    48181 tacctcaaag agatgcaagg gtaacgcaaa atcgtgacaa ataaaaaacc cggtcgcgag
    48241 aaaccaccgg gtttgataat tatcctaggg aaatcagcgt ttgctgatct gatcgaacgt
    48301 accgccgtta gcaaaatgct ctttttgcgc tttcgtccag ccgccgaact cttcatcaat
    48361 ggtgaataac ttcagctttg gaaacgcatt ttcgtacttt ttcgctactt cagcgtcgcg
    48421 cggacggtag tagtttttcg cggcaatttc ctgaccttct ggcgagtaga gatatttcag
    48481 gtaggcttcc gccacctctt tggtgccttt tttctcgacc actttatcga ccaccgacac
    48541 cgttggctct gcgaggatag actcactcgg cgtgacgatt tcgaatttat ctttccccag
    48601 ttcattcgct gccagcagag cttcgttttc ccaggcgatc agtacatcgc caatcccgcg
    48661 ctcgacaaag gtgttagtgg agccgcgcgc gccagaatcc agaacttcga cgtttttata
    48721 cagtgcccga acaaaatcct gtgcttttgc ctgatcgttg ttgttgtgat gcagcgcgta
    48781 gccccaggca gccaggtagc tccagcgtgc gccaccagag cttttcggat taggcgtgat
    48841 caccgaaaca cccggtttaa tcagatcgtt ccagtcatgg atctgcttcg gattgccctt
    48901 acgcaccagg aaaacaatgg tggaagtgta cggtgcggag ttatccggca gacgtttgat
    48961 ccactcttta tcaatccgcc cgcgttccgc aatcgcgtcc acgtcatagg ccagagccag
    49021 cgtgacaaca tcagcttcaa taccgttgat taccgacgtc gcttgtttac ctgaaccacc
    49081 gtgtgactga cgaatcacca cgttatcacc agtttgctgt ttccagtggg cgctgaatgc
    49141 cttgttgtac tgttcgtaca attcgcgcgt tggatcatat gaaacgttaa gaagctgaat
    49201 atcctttgcc ataacgctgg ttgccgccag caaaaatgtt aaccctacgc cccacttgtt
    49261 catcgcccga ctctcttatg ttgtgttgtg atgagcaaag cgtgccagaa ggttaaccaa
    49321 acattaaaga ataaaaaaag attggctata acttgcgggt atatgttgag ggattaaaaa
    49381 ggcggaagaa atccgcctca tattgctgac aaagtgcgct ttgtccatgc cggatgcggc
    49441 gcgaacgcct tatccggcct acaaaagttt gcaaattcaa taaattgcag aattcatgta
    49501 ggcctgataa gcgaagcgca tcaggcattt ttgcttctgt catcggtttc aggctaaagg
    49561 aatctgcctt tttccgaaat cattaataca gttttttcgc gcagtccagc cagtcacctt
    49621 tgaacggacg cttcatgttt tcgatagcgt cgatgatgtc gtggtgaacc agctgttcgt
    49681 tctggatacc tacacaacga ccgccgtaac ctgccagcag cagatcgata gcgtaagcgc
    49741 ccatacggga agccagaata cggtcgtaag gcaccggaga accaccgcgc tggatgtggc
    49801 ccagcacagt tgcgcgggtt tcacgaccgg tttctttctc gatgaaatgc gccagttcgt
    49861 caacatcaca catatgttcg gtaatcgcca cgatcgcgtg ttttttacct ttcgcgatac
    49921 ccgctttgat ttcgtttacc aggtcttcac ggctgaattc aacttccgga acgacaacga
    49981 attcacagcc accggcaatg gccgcagcca aggtcagatc gccacaataa cggcccatca
    50041 cttccaccac ggaaatacgc tggtgagaag aagaggtgtc acgcagacgg tcgatcgctt
    50101 ctacaacggt gctcagcgca gtgaagaaac cgatagtgta gtcagtgcct ttgatgtcgt
    50161 tgtcgatagt gcccggcaga ccgatgcacg ggaagcccat ttcggtcaga cgcattgcac
    50221 ccatgtagga accgtcaccg ccgataacca ccagcgcgtc gataccacgt tttttcaggt
    50281 tttcgatagc cacggcgcgg atgttctcgt cgcggaattc cgggaaacgc gcagaaccga
    50341 ggaacgtacc gccacggttg atcatgtcag acacgctgta acggtctagc tgtaccatac
    50401 ggtcttcata cagacccaga tagccgtcat aaatacccat tacttccaga ccttctgtca
    50461 gcgcagaacg aacaaccccg cgaattgcgg cgttcatgcc tggcgcatca ccgccgcttg
    50521 tcaacacacc tattttctta atcatgacta cctctgaact ttggaatgca aaatgaaatc
    50581 tgttgccgga agtcttcttg cacatcgaag tgatccaacg aatgtgcaaa tagtataaca
    50641 atcacttcct gctgaattga ttcaggtcag gccaaatggc ggtattttat acacaaaatg
    50701 cgggtctggc tctcttttat actgattatg aaagcataga ccgtttaccc tccctgggta
    50761 cgacggaaca ggggtcctga tggataatca catccgatcc cggaaaacgc cgtaaaatag
    50821 cctgctctac ctgatccgcc accatatgtg cctgaaccaa aggcagagag tcttccattt
    50881 ccaaatgaat ctgaataaag cgggtcggcc ctgactgccg cgtgcgaaga tcgtgagcgc
    50941 cgctaacacc cggccaggaa gtcacgatat caataatttc ttgccgttct tcatcaggca
    51001 atgcgcgatc cagtaatgac tgtaccgcct catatcccat gcgtaacgcg ctatataaaa
    51061 tatagatgcc gattcccaat gcaaacagag catcggcgcg atgccagccg taccaagaca
    51121 accccagcgc cagcagaatt gcgccgttca tcataacatc agactggtaa tgtagcatat
    51181 cagcccgcac cgcctggctt tgcgtccggc gcaccaccca ccgctgaaac gagacaagga
    51241 taatcgtaca aattagcgcc acaattgtca cgataacccc gacgcctgga tctgtcatcg
    51301 gtgttggaga taccagatgt tgaatacccg tcaaaaacag gaatagtgcc gaaccggaga
    51361 taaacatact ttgcgccagc gccgcgaggg actcagcttt accgtgacca aacgagtgat
    51421 tatcgtcggc aggttgcagg gaatatcgca ccaccagtaa attcgtcaac gacgcgccga
    51481 tatccaccag cgaatccacc agcgcggcga gaatactcac cgacccggta taccaccatg
    51541 ccaaaatttt aatcagcaat agcaacgaag ccatcgccgt cgcagcaatc gccgcccgac
    51601 tgaccagccg tccataagat tgattcataa atactcccgc tatcaactga cgctagtata
    51661 acggaagcaa atcatctgca atgcattaag cagcaggcaa attgaggata aaaaaaaaac
    51721 cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca gggtttacta
    51781 ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt gccattgcgt
    51841 cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg cttgctgctc
    51901 cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct gacgagcaat
    51961 ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa aattttctgc
    52021 ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct gtttgtgccg
    52081 ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac ttatgccgtc
    52141 gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc agttatcgcc
    52201 tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg ccatgacggc
    52261 agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc aacgatagag
    52321 agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta aagtcatgga
    52381 ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg aataaaatcc
    52441 tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc gagatggaag
    52501 gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg gacgacagca
    52561 ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac acattaaaag
    52621 cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc agtgaacttg
    52681 atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg tttaatgatc
    52741 gtgagctggt ggcacgtatt cgcgcgatcc tgcgccgttc gcactggagc gagcaacagc
    52801 aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg aatccaggcc
    52861 gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag tttaccctgc
    52921 tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta agccaggaag
    52981 tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt tccaacctgc
    53041 gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg cgtggtcgcg
    53101 gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct tcgccatctt
    53161 ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg ttacccaagc tcgattcacg
    53221 ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg agcagcatgt
    53281 cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc gtctgttccg
    53341 ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca ccgaaggccg
    53401 cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg gtcaggccga
    53461 taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg gtccgttctc
    53521 cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca gcagttctca
    53581 atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg tcaccatgtt
    53641 ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg cgcgtaagct
    53701 gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg aactggaagc
    53761 ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca ccgcgctgga
    53821 gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc tgcgcacccc
    53881 gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg aaagcaagga
    53941 actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg atctgttggt
    54001 gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag ccaaccagtt
    54061 gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca agtcgttgac
    54121 agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccaaacgccc tggaaagtgc
    54181 gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg aagtgggctt
    54241 tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg gcgttagccc
    54301 ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac gcgatcgtga
    54361 atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc agcatcgtgg
    54421 ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt ggttgccgct
    54481 gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg ccgcatccgg
    54541 catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta cactccgggg
    54601 gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt cggcgctttt
    54661 gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg tgatactgat
    54721 cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc acatcgctga
    54781 cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctagcgct atcacgctat
    54841 acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg atatcaaaat
    54901 ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat gcctctcccc
    54961 agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt gagagatttt
    55021 cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc acccagtaga
    55081 gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga acctttttct
    55141 ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca tcaacctgga
    55201 ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct gtgtatacat
    55261 caaccggata tcgcatctgt ttcctctcca cgagaaattt ccccattaga ccatatttat
    55321 tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa tagcaaaccg
    55381 gacgaacgtt agttcatccg gctgtgggga ttaaggaagc aggtttattt caccgtacct
    55441 aaaatttcga ttttcgctgc tctggctttg actttacgtg accagataga ggtaagaatt
    55501 ggcaccagaa tcgaagtcac aatgaccgcc gtcactacca gcgaagttgc tgccggagcc
    55561 atcggtttaa acgcaggcac catttctgca atcagcacag gtgtcgctac cgcggcccct
    55621 gcggaactgg aagcggcaat tccggcagtg ccatcgccac cgccaatcaa tttatcggcg
    55681 ataatcaacg gaataccggt cacgataatt actgccacac ccaacaggat ccccagcaaa
    55741 ccagtctggg caatcacagt caaatcaatg gtattgccca gcgcgaaggc aaagaatgga
    55801 atcagcgttt gcaccgcttt gctgaaaaat tctcgtaatt cagggtcaag attccccagg
    55861 gcaaagccca ccaggaacgg taatactgcg ccgacgaaaa catgcggttc aaacgaggca
    55921 atcccggcag tgcccagaat aatcatcgtc atgagcggcc cggactccaa cgacatcaac
    55981 acaaatgccc cagcttcttc tttcgtaccg tactgctgca tgatggaagc gtaaagtccg
    56041 ccgttggtca tatccatcgc cgccaccagc gccagcgttg aaagtccggc aaagaatcca
    56101 acttcaacac catgttccgg aataatgcgc gaggcaattg ccgcaaccac ccacgcgacg
    56161 gcaattttag ttaccaccag cgtaccggat ttacgcagta ccgttcccgt cgcgcttaat
    56221 tttattgacg cccccatgca aaaaaaccac accgccagaa tgggcaccgt accggtaatc
    56281 atcccgttgg tgaatgatcc aaaatatttc cccgcccccg gcgagaaggt gtggcacagt
    56341 gcgccaagga atagcgggac gagcatcatc ccccccggga ttttctcaat cgagcgtttt
    56401 atctgcattt ccatcacctg tcggcatatt tttaagggct gatgcgtaat tcctcaatgg
    56461 agcatcagtg ttgatggaaa cgaagataga cattcattta atcaataaaa gtgatcaaaa
    56521 ccacatcaat tgaaacgctg ttttattttt ataggatcat tgtttttatt ttggaattga
    56581 tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt aagctgatat gcggcctata
    56641 cgcctcattg cagcaggcgg caaatgatta ttttttcgcc gcaaaacgtg ccgctgcttc
    56701 gtcccagttc accacgttcc agaactcttt gatgtagtcc ggacggcggt tctggaattt
    56761 caggtagtaa gcatgttccc acacatccag gcccagaatc gggaagccgg aagcgccaga
    56821 aatagcttca cccatcagcg gagaatcctg gttagcagta gaaaccaccg ccagtttatc
    56881 gcctttcagc accagccatg cccagccgga accaaagcgg gaagctgccg ctttttcaaa
    56941 ttctgctttg aagttatcaa cggagccgaa gtcacgttcg atagccgctt tcaggtcacc
    57001 ctgcagggtg gtgccttttt tcagaccttt ccagaacagg ctgtggttag cgtgaccgcc
    57061 agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc tggtccagtt tggtgatcag
    57121 ctcttcaacc ggcaggttgg caaattctgg caggctttcc agcgccgcgt tggcgttgtt
    57181 tacgtaggtc tgatggtgtt tggtgtggtg gatttccatg gtctgcttat cgaagtgcgg
    57241 ttccagggca tcgtaagcat acggcaggga tggcagggta tagctcatat tcatctccag
    57301 tattgtcggg cggccgattg ttaatgccgc gtaagcagtt ggttcattat agttaattaa
    57361 atgatattga aaatgattat caatgccgta cttttcgtaa gggtatggtt ttgcaggaaa
    57421 atgcccgaga tgtgaagcaa atcacccact taatgccgtg attgccagta aatcgacaac
    57481 ggcggcaaca ggcgaaaggt taatcgacag cacgattttt acactcatct cgtcggagat
    57541 gtgaagcgac gaaaaatgat gaggataaga agatgagtaa cgcgattacg atggggatat
    57601 tttggcattt gatcggcgcg gccagtgcag cctgttttta cgctccgttc aaaaaagtaa
    57661 aaaaatggtc atgggaaacc atgtggtcag tcggtgggat tgtttcgtgg attattctgc
    57721 cgtgggccat cagcgccctg ttactaccgg atttctgggc gtattacagc tcgtttagtc
    57781 tctctacgct gctgcctgtt tttctgttcg gcgctatgtg gaggatcggt aatatcaact
    57841 atggcctgac catgcgttat ctcggcatgt cgatgggaat tggcatcgcc attggcatta
    57901 cgttgattgt cggtacgctg atgacgccaa ttatcaacgg caatttcgat gtgctgatta
    57961 acaccgaagg cggacgcatg acgttgctcg gcgttctggt ggcgctgatt ggcgtaggga
    58021 ttgtaactcg agccgggcag ttgaaagagc gcaagatggg cattaaagcc gaagagttca
    58081 atctgaaaaa agggctggtg ctggcggtga tgtgcggcat tttctctgcc gggatgtcct
    58141 ttgcgatgaa cgccgcaaaa ccgatgcatg aagccgctgc cgcacttggc gtcgatccac
    58201 tgtatgtcgc tctgccaagc tatgttgtca tcatgggcgg cggcgcgatc attaacctcg
    58261 gtttctgttt tattcgtctg gcaaaagtga aggatttgtc gctaaaagcc gacttctcgc
    58321 tggcaaaacc gctgatcatt cacaatgtgt tactctcggc actgggcggg ttgatgtggt
    58381 atctgcaatt ctttttctat gcctagggcc acgcccgcat tccggcgcag tatgactaca
    58441 tcagttggat gctgcatatg agtttctatg tattgtgcgg cggtatcgtc gggctggtgc
    58501 tgaaagagtg gaacaataca ggacgccgtc cggtaacggt gttgagcctc ggttgtgtgg
    58561 tgattattgt cgccgccaac atcgtcggca tcggcatggc gaattaatct ttctgcgaat
    58621 tgagatgacg ccactggctg ggcgtcatcc cggtttcccg ggtaaacacc accgaaaaat
    58681 agttactatc ttcaaagcca cattcggtcg aaatatcact gattaacagg cggctatgct
    58741 ggagaagata ttgcgcatga cacactctga cctgccgcag atattgattg atggtcattc
    58801 cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact gcacgatgcc tcatcacaaa
    58861 atttatccag cgcaaaggga cttttcaggc tagccgccag ccgggtaatc agcttatcca
    58921 gcaacgtttc gctggatgtt ggtggcaacg aatcactggt gtaacgatgg cgattcagca
    58981 acatcaccaa ctgcccgaac agcaactcag ccatttcgtt agcaaacggc acatgctgac
    59041 tactttcatg ctcaagctga ccgataacct gccgcgcctg cgccatcccc acgctaccta
    59101 agcgccagtg tggttgccct gcgctggcgc taaatcccgg aatcgctccc tgccagtcaa
    59161 gattcagctt cagccgctcc gggcaataaa taatattctg caaaaccaga tcgttaacgg
    59221 aagcgtagga gtgtttatcg tcagcatgaa tataaaagag atcgccacgg gtaatgcgat
    59281 aaggacgatc gttgagtaca tgcagaccat taccgcgcca gacaatcacc agctcacaaa
    59341 aatcatgtgt atgttcggca aagacatctt gcggataacg gtcagccaca gcgactgcct
    59401 gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa ctgatgcgcc accgtggcta
    59461 cctcggccag agaacgaagt tgattattcg caatatggcg tacaaatacg ttgagaagat
    59521 tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat cacgcggtga ccagttaaac
    59581 tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga atccacagcg ataggcgatg
    59641 tcagtgacgc tggcctcgct gtggcgtagc agatgtcggg ctttcatcag tcgcaggcgg
    59701 ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg tagcgtacgc
    59761 agtgaaagag aaaattgatc cgccacggca tcccaattca cctcatcggc aaaatggtcc
    59821 tccagccagg ccagaagcaa gttgagacgt gatgcgctgt tttccaggtt ctcctgcaaa
    59881 ctgcttttac gcagcaagag cagtaattgc ataaacaaga tctcgcgact ggcggtcgag
    59941 ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa ccagctgtcg cacctgctgc
    60001 aatacgctgt ggttaacgcg ccagtgagac ggatactgcc catccagctc ttgtggcagc
    60061 aactgattca gcccggcgag aaactgaaat cgatccggcg agcgatacag cacattggtc
    60121 agacacagat tatcggtatg ttcatacaga tgccgatcat gatcgcgtac gaaacagacc
    60181 gtgccaccgg tgatggtata gggctgccca ttaaacacat gaatacccgt gccatgttcg
    60241 acaatcacaa tttcatgaaa atcatgatga tgttcaggaa aatccgcctg cgggagccgg
    60301 agttctatcg ccacggacgc gttacaagac ggaaaaaaat ccacactatg taatacggtc
    60361 atactggcct cctgatgtcg tcaacacggc gaaatagtaa tcacgaggtc aggttcttac
    60421 cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt tttcaagata cagcgtgaat
    60481 tttcaggaaa tgcggtgagc atcacatcac cacaattcag caaattgtga acatcatcac
    60541 gttcatcttt ccctggttgc caatggccca ttttcctgtc agtaacgaga aggtcgcgaa
    60601 ttcaggcgct ttttagactg gtcgtaatga aattcagcag gatcacatta tgacctttcg
    60661 caattgtgtc gccgtcgatc tcggcgcatc cagtgggcgc gtgatgctgg cgcgttacga
    60721 gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt tttaacaatg ggctgcatag
    60781 ccaaaacggt tatgtcacct gggatgtgga tagcctggaa agtgccattc gccttggatt
    60841 aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt gggattgata cctggggcgt
    60901 ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc ctgcccgttg cttatcgcga
    60961 tagccgcacc aatggcctga tgccgcaggc acaacagcaa ctcggcaaac gcgatattta
    61021 tcagcgtagc ggcatccagt ttctaccctt caatacgctt tatcagttgc gtgcgctgac
    61081 ggagcaacaa cctgaactta ttccacacat tgctcacgct ctgctgatgc cggattactt
    61141 cagttatcgc ctgaccggca agatgaactg ggaatatacc aatgccacca ccacacaact
    61201 ggtcaatatc aatagcgacg actgggacga gtcgctactg gcgtggagcg gggccaacaa
    61261 agcctggttt ggtcgcccga cgcatccggg taatgtcatt gggcactgga tttgcccaca
    61321 gggtaatgag attccggtgg tcgccgttgc cagccatgat accgccagcg cggttatcgc
    61381 ctcgccgtta aacggttcac gcgccgctta tctctcttct ggcacctggt cattgatggg
    61441 cttcgaaagc cagacgccat ttaccaatga cacggcgctg gcagccaaca tcaccaatga
    61501 aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt atgggcttat ggctgcttca
    61561 gcgagtgctt caggaacggc aaatcaacga tctcccggcg cttatcgccg cgacacaggc
    61621 acttccggcc tgccgcttca tcatcaatcc caatgacgat cgctttatta atcctgacga
    61681 gatgtgcagc gaaattcagg ctgcgtgtcg ggaaacggcg caaccgatcc cgggaagtga
    61741 tgctgaactg gcgcgctgta ttttcgacag tctggcgctg ctgtatgccg atgtgttgca
    61801 tgagctggcg cagctgcgcg gtgaagattt ctcgcaactg catattgtcg gcggcggctg
    61861 ccagaacacg ctgctcaacc agctatgcgc cgatgcctgc ggtattcggg tgatcgccgg
    61921 gcctgttgaa gcctcaacgc tcggcaatat cggcatccag ttaatgacgc tggatgaact
    61981 caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc gcgaatctga ccacctttac
    62041 ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag attcactcta cacgacagac
    62101 aaaggagctt tgcgcatgac cactcaactg gaacaggcct gggagctggc gaaacagcgt
    62161 ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc aacttgatcg tttacccgtt
    62221 tcaatgcact gctggcaggg cgatgatgtt tccggttttg aaaacccgga aggttcgctg
    62281 accgggggga ttcaggccac aggcaattat ccgggcaaag cgcgtaatgc cagtgagcta
    62341 cgtgccgatc tggaacaggc tatgcggctg attccggggc cgaaacggct taatttacat
    62401 gccatctatc tggaatcaga tacgccagtc tcgcgcgacc agatcaaacc agagcacttc
    62461 aaaaactggg ttgaatgggc gaaagccaat cagctcggtc tggattttaa cccctcctgc
    62521 ttttcgcatc cgctaagcgc cgatggcttt acgctctcgc atcccgacga cagcattcgc
    62581 cagttctgga ttgatcactg caaagccagc cgtcgcgttt cggcctattt tggcgagcaa
    62641 ctcggcacac catcggtgat gaacatctgg atcccggatg gtatgaaaga tatcaccgtt
    62701 gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg atgaggtgat cagcgagaag
    62761 ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat tgtttggcat tggcgcagag
    62821 agctacacgg ttggctccaa tgagttttac atggggtatg ccaccagccg ccagactgcg
    62881 ctgtgcctgg acgccgggca cttccacccg actgaagtga tttccgacaa gatttccgcc
    62941 gccatgctgt atgtgccgca gttgctgctg cacgtcagcc gtccggttcg ctgggacagc
    63001 gatcacgtag tgctgctgga tgatgaaacc caggcaattg ccagtgagat tgtgcgtcac
    63061 gatctgtttg accgggtgca tatcggcctt gacttcttcg atgcctctat caaccgcatt
    63121 gccgcgtggg tcattggtac acgcaatatg aaaaaagccc tgctgcgtgc gttgctggaa
    63181 cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt acactgcgcg tctggcactg
    63241 ctggaagagc agaaatcgtt gccgtggcag gcggtctggg aaatgtattg ccaacgtcac
    63301 gatacgccag caggtagcga atggctggag agcgtgcggg cttatgagaa agcgattttg
    63361 agccagcgtg ggtaaacact gccggatgcg gcacgagcgc cttatccggc ctacgggtcg
    63421 aatgtccatg cgggctgtca ccctcacccc ggccctctcc ctgaaagggc gagggagaaa
    63481 aacatagcct cgtgcggcaa cggagctgaa caccgctccg gacagtcccc tcgcccctac
    63541 ggggagaggg ttagggtgag gggaacaagc cgcaccatgt ctaaacaagg caacctaatt
    63601 tacaggaaca cagaacatgc aaaacattac ccagtcctgg tttgtccagg gaatgatcaa
    63661 agccaccacc gatgcctggc tgaaaggctg ggacgagcgc aacggcggca acctgacgct
    63721 gcgcctggat gacgccgata tcgcgcttta tcacgacaac ttccacccac aaccgcgcta
    63781 tattccgctc agccaaccca tgcctttact ggcaaatacg ccgtttattg tcaccggctc
    63841 cggcaaattc ttccgtaacg tccagctcga tcctgcagct aacttaggcg tcgtaaaagt
    63901 cgacagcgat ggcgcgggct accatattct ctgggggtta accaacgaag ccgttccaac
    63961 ttctgaactt ccggctcact ttctctccca ctgcgagcgc attaaagcca ccaacggtaa
    64021 agatcgggtg atcatgcact gccacgccac caacctgatc gccctcacct atgtactgga
    64081 aaacgacacc gcggtcttta ctcgccaact gtgggaaggc agcaccgaat gtctggtagt
    64141 attcccggat ggcgttggca ttctgccgtg gatggtgccc ggcacggacg aaccggccag
    64201 gcgaccgcac aagagatgca aaaacattcg ctggtgttgt ggcccttcca cggtgtgttc
    64261 ggttccggac cgacgctgga tgaagccttt ggtttaatcg acaccgcaga aaaatcagca
    64321 caagtattag tgaaggttta ttcgatgggc ggcatgaaac agaccatcag ccgtgaagag
    64381 ttgatagcgc tcggcaagcg cttcgccgtt acaccactag ccagtgcgct ggcgctgtaa
    64441 ggagcaaaca tgatccgcaa agcctttgtc atgcaggtaa accccgacgc ccacgaagag
    64501 tatcagcgtc ggcataatcc catctggccg gaactggaag cagtgttgaa atctcacggt
    64561 gtgcataact acgccattta cctcgacaaa gcgcgtaatc tgctgtttgc catggtagag
    64621 attgaatctg aagaacggtg gaatgcggta gccagtactg atgtttgcca acgttggtgg
    64681 aaatatatga ccgatgttat gcccgctaac ccagataaca gcccggtgag tagcgagctg
    64741 caagaagtgt tttacctgcc gtaattgccc aatgtgccgg agatgcctcc ggcacaatat
    64801 tttaaatttc gatttgaaaa attaaacata ttacatctcc cggtaagtta tatttccctg
    64861 atacattgtg agtaaatcac aaaaataatg aataacccat taatgattca tgtggtttat
    64921 ttaaataacc attatgtgca ttactccgca aatctgacct ttcactctgt ctcaaattgt
    64981 cattgtttac cgacatatcg cctctcatc
//
LOCUS       DBKEKI010000005        62421 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.005, whole genome shotgun sequence.
ACCESSION   DBKEKI010000005 DBKEKI010000000
VERSION     DBKEKI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.005"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..111
                     /locus_tag="ACUQPX_000300"
     CDS             <1..111
                     /locus_tag="ACUQPX_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HHV9055103.1"
                     /translation="YRNKQYHLPGDTADRLNYQKMAQVVDGVITLLYNSK"
     gene            214..717
                     /locus_tag="ACUQPX_000301"
     CDS             214..717
                     /locus_tag="ACUQPX_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HHV9055104.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(784..2241)
                     /gene="nuoN"
                     /locus_tag="ACUQPX_000302"
     CDS             complement(784..2241)
                     /gene="nuoN"
                     /locus_tag="ACUQPX_000302"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HHV9055105.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2248..3777)
                     /gene="nuoM"
                     /locus_tag="ACUQPX_000303"
     CDS             complement(2248..3777)
                     /gene="nuoM"
                     /locus_tag="ACUQPX_000303"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HHV9055106.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3941..5782)
                     /gene="nuoL"
                     /locus_tag="ACUQPX_000304"
     CDS             complement(3941..5782)
                     /gene="nuoL"
                     /locus_tag="ACUQPX_000304"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HHV9055107.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5779..6081)
                     /gene="nuoK"
                     /locus_tag="ACUQPX_000305"
     CDS             complement(5779..6081)
                     /gene="nuoK"
                     /locus_tag="ACUQPX_000305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HHV9055108.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6078..6632)
                     /gene="nuoJ"
                     /locus_tag="ACUQPX_000306"
     CDS             complement(6078..6632)
                     /gene="nuoJ"
                     /locus_tag="ACUQPX_000306"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HHV9055109.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6644..7186)
                     /gene="nuoI"
                     /locus_tag="ACUQPX_000307"
     CDS             complement(6644..7186)
                     /gene="nuoI"
                     /locus_tag="ACUQPX_000307"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HHV9055110.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7201..8178)
                     /gene="nuoH"
                     /locus_tag="ACUQPX_000308"
     CDS             complement(7201..8178)
                     /gene="nuoH"
                     /locus_tag="ACUQPX_000308"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HHV9055111.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8175..10901)
                     /gene="nuoG"
                     /locus_tag="ACUQPX_000309"
     CDS             complement(8175..10901)
                     /gene="nuoG"
                     /locus_tag="ACUQPX_000309"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HHV9055112.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10954..12291)
                     /gene="nuoF"
                     /locus_tag="ACUQPX_000310"
     CDS             complement(10954..12291)
                     /gene="nuoF"
                     /locus_tag="ACUQPX_000310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HHV9055113.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12288..12788)
                     /gene="nuoE"
                     /locus_tag="ACUQPX_000311"
     CDS             complement(12288..12788)
                     /gene="nuoE"
                     /locus_tag="ACUQPX_000311"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HHV9055114.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12791..14593)
                     /gene="nuoC"
                     /locus_tag="ACUQPX_000312"
     CDS             complement(12791..14593)
                     /gene="nuoC"
                     /locus_tag="ACUQPX_000312"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HHV9055115.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPTAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14687..15349)
                     /gene="nuoB"
                     /locus_tag="ACUQPX_000313"
     CDS             complement(14687..15349)
                     /gene="nuoB"
                     /locus_tag="ACUQPX_000313"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HHV9055116.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15365..15808)
                     /gene="nuoA"
                     /locus_tag="ACUQPX_000314"
     CDS             complement(15365..15808)
                     /gene="nuoA"
                     /locus_tag="ACUQPX_000314"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HHV9055117.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16439..17377)
                     /gene="lrhA"
                     /locus_tag="ACUQPX_000315"
     CDS             complement(16439..17377)
                     /gene="lrhA"
                     /locus_tag="ACUQPX_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HHV9055118.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKSGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18297..19514
                     /gene="alaA"
                     /locus_tag="ACUQPX_000316"
     CDS             18297..19514
                     /gene="alaA"
                     /locus_tag="ACUQPX_000316"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HHV9055119.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19598..20197
                     /gene="yfbR"
                     /locus_tag="ACUQPX_000317"
     CDS             19598..20197
                     /gene="yfbR"
                     /locus_tag="ACUQPX_000317"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HHV9055120.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20256..22088)
                     /locus_tag="ACUQPX_000318"
     CDS             complement(20256..22088)
                     /locus_tag="ACUQPX_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HHV9055121.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASTAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22175..22825)
                     /gene="hxpA"
                     /locus_tag="ACUQPX_000319"
     CDS             complement(22175..22825)
                     /gene="hxpA"
                     /locus_tag="ACUQPX_000319"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HHV9055122.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(22836..23330)
                     /locus_tag="ACUQPX_000320"
     CDS             complement(22836..23330)
                     /locus_tag="ACUQPX_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HHV9055123.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23413..23868)
                     /gene="yfbV"
                     /locus_tag="ACUQPX_000321"
     CDS             complement(23413..23868)
                     /gene="yfbV"
                     /locus_tag="ACUQPX_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HHV9055124.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24206..25408
                     /gene="ackA"
                     /locus_tag="ACUQPX_000322"
     CDS             24206..25408
                     /gene="ackA"
                     /locus_tag="ACUQPX_000322"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HHV9055125.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25483..27627
                     /gene="pta"
                     /locus_tag="ACUQPX_000323"
     CDS             25483..27627
                     /gene="pta"
                     /locus_tag="ACUQPX_000323"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HHV9055126.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27817..29337
                     /gene="yfcC"
                     /locus_tag="ACUQPX_000324"
     CDS             27817..29337
                     /gene="yfcC"
                     /locus_tag="ACUQPX_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HHV9055127.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMDSSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29370..29912)
                     /gene="yfcD"
                     /locus_tag="ACUQPX_000325"
     CDS             complement(29370..29912)
                     /gene="yfcD"
                     /locus_tag="ACUQPX_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HHV9055128.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29970..30521)
                     /gene="yfcE"
                     /locus_tag="ACUQPX_000326"
     CDS             complement(29970..30521)
                     /gene="yfcE"
                     /locus_tag="ACUQPX_000326"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HHV9055129.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30577..31221)
                     /gene="yfcF"
                     /locus_tag="ACUQPX_000327"
     CDS             complement(30577..31221)
                     /gene="yfcF"
                     /locus_tag="ACUQPX_000327"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HHV9055130.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31357..32004
                     /gene="yfcG"
                     /locus_tag="ACUQPX_000328"
     CDS             31357..32004
                     /gene="yfcG"
                     /locus_tag="ACUQPX_000328"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HHV9055131.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKWLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32061..32423
                     /gene="folX"
                     /locus_tag="ACUQPX_000329"
     CDS             32061..32423
                     /gene="folX"
                     /locus_tag="ACUQPX_000329"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HHV9055132.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32444..33337
                     /locus_tag="ACUQPX_000330"
     CDS             32444..33337
                     /locus_tag="ACUQPX_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HHV9055133.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGIDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33385..34275)
                     /gene="rpnB"
                     /locus_tag="ACUQPX_000331"
     CDS             complement(33385..34275)
                     /gene="rpnB"
                     /locus_tag="ACUQPX_000331"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HHV9055134.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEETLRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34472..35245)
                     /gene="hisP"
                     /locus_tag="ACUQPX_000332"
     CDS             complement(34472..35245)
                     /gene="hisP"
                     /locus_tag="ACUQPX_000332"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HHV9055135.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35253..35969)
                     /locus_tag="ACUQPX_000333"
     CDS             complement(35253..35969)
                     /locus_tag="ACUQPX_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9055136.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILILHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(35966..36652)
                     /gene="hisQ"
                     /locus_tag="ACUQPX_000334"
     CDS             complement(35966..36652)
                     /gene="hisQ"
                     /locus_tag="ACUQPX_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HHV9055137.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(36742..37524)
                     /gene="hisJ"
                     /locus_tag="ACUQPX_000335"
     CDS             complement(36742..37524)
                     /gene="hisJ"
                     /locus_tag="ACUQPX_000335"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HHV9055138.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(37745..38527)
                     /gene="argT"
                     /locus_tag="ACUQPX_000336"
                     /pseudo
     CDS             complement(37745..38527)
                     /gene="argT"
                     /locus_tag="ACUQPX_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
     gene            complement(38793..39362)
                     /gene="ubiX"
                     /locus_tag="ACUQPX_000337"
     CDS             complement(38793..39362)
                     /gene="ubiX"
                     /locus_tag="ACUQPX_000337"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HHV9055139.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39457..40974)
                     /gene="purF"
                     /locus_tag="ACUQPX_000338"
     CDS             complement(39457..40974)
                     /gene="purF"
                     /locus_tag="ACUQPX_000338"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HHV9055140.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGVIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41011..41499)
                     /gene="cvpA"
                     /locus_tag="ACUQPX_000339"
     CDS             complement(41011..41499)
                     /gene="cvpA"
                     /locus_tag="ACUQPX_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HHV9055141.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(41758..42420)
                     /gene="dedD"
                     /locus_tag="ACUQPX_000340"
     CDS             complement(41758..42420)
                     /gene="dedD"
                     /locus_tag="ACUQPX_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HHV9055142.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42410..43678)
                     /gene="folC"
                     /locus_tag="ACUQPX_000341"
     CDS             complement(42410..43678)
                     /gene="folC"
                     /locus_tag="ACUQPX_000341"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HHV9055143.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELLE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWVFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(43748..44662)
                     /gene="accD"
                     /locus_tag="ACUQPX_000342"
     CDS             complement(43748..44662)
                     /gene="accD"
                     /locus_tag="ACUQPX_000342"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HHV9055144.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(44818..45477)
                     /locus_tag="ACUQPX_000343"
     CDS             complement(44818..45477)
                     /locus_tag="ACUQPX_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HHV9055145.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(45560..46372)
                     /gene="truA"
                     /locus_tag="ACUQPX_000344"
     CDS             complement(45560..46372)
                     /gene="truA"
                     /locus_tag="ACUQPX_000344"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HHV9055146.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46372..47385)
                     /locus_tag="ACUQPX_000345"
     CDS             complement(46372..47385)
                     /locus_tag="ACUQPX_000345"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HHV9055147.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERCIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47451..48587)
                     /gene="pdxB"
                     /locus_tag="ACUQPX_000346"
     CDS             complement(47451..48587)
                     /gene="pdxB"
                     /locus_tag="ACUQPX_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HHV9055148.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQVRLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            48686..49681
                     /gene="flk"
                     /locus_tag="ACUQPX_000347"
     CDS             48686..49681
                     /gene="flk"
                     /locus_tag="ACUQPX_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HHV9055149.1"
                     /translation="MIQPISGPPPRQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(49678..50856)
                     /locus_tag="ACUQPX_000348"
     CDS             complement(49678..50856)
                     /locus_tag="ACUQPX_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HHV9055150.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51131..52351)
                     /gene="fabB"
                     /locus_tag="ACUQPX_000349"
     CDS             complement(51131..52351)
                     /gene="fabB"
                     /locus_tag="ACUQPX_000349"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HHV9055151.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            52510..54516
                     /gene="mnmC"
                     /locus_tag="ACUQPX_000350"
     CDS             52510..54516
                     /gene="mnmC"
                     /locus_tag="ACUQPX_000350"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HHV9055152.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(54637..54915)
                     /locus_tag="ACUQPX_000351"
     CDS             complement(54637..54915)
                     /locus_tag="ACUQPX_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HHV9055153.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(54949..55497)
                     /gene="epmC"
                     /locus_tag="ACUQPX_000352"
     CDS             complement(54949..55497)
                     /gene="epmC"
                     /locus_tag="ACUQPX_000352"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HHV9055154.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55497..56306)
                     /locus_tag="ACUQPX_000353"
     CDS             complement(55497..56306)
                     /locus_tag="ACUQPX_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HHV9055155.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEYRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56306..57130)
                     /gene="mepA"
                     /locus_tag="ACUQPX_000354"
     CDS             complement(56306..57130)
                     /gene="mepA"
                     /locus_tag="ACUQPX_000354"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HHV9055156.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57134..58219)
                     /gene="aroC"
                     /locus_tag="ACUQPX_000355"
     CDS             complement(57134..58219)
                     /gene="aroC"
                     /locus_tag="ACUQPX_000355"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HHV9055157.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58254..59186)
                     /gene="prmB"
                     /locus_tag="ACUQPX_000356"
     CDS             complement(58254..59186)
                     /gene="prmB"
                     /locus_tag="ACUQPX_000356"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HHV9055158.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNQWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTCRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59352..59903
                     /gene="smrB"
                     /locus_tag="ACUQPX_000357"
     CDS             59352..59903
                     /gene="smrB"
                     /locus_tag="ACUQPX_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HHV9055159.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60007..60897)
                     /locus_tag="ACUQPX_000358"
     CDS             complement(60007..60897)
                     /locus_tag="ACUQPX_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HHV9055160.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSVAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(60884..61408)
                     /locus_tag="ACUQPX_000359"
     CDS             complement(60884..61408)
                     /locus_tag="ACUQPX_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9055161.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPVNNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61405..61875)
                     /locus_tag="ACUQPX_000360"
     CDS             complement(61405..61875)
                     /locus_tag="ACUQPX_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9055162.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(61872..62420)
                     /locus_tag="ACUQPX_000361"
     CDS             complement(61872..62420)
                     /locus_tag="ACUQPX_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9055163.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
BASE COUNT        15558 a        17013 c        15619 g        14231 t
ORIGIN      
        1 tatcgtaata aacaatacca cttgcccggt gataccgcag acagattgaa ttatcagaaa
       61 atggctcagg tggtagatgg tgttataacc ttgttataca acagtaaata ataaaaattt
      121 ctcgaggcgg gtccagaaac agcgacatgt acattttatt ttccctcgtc tgttttaaga
      181 tgcgaaacat aaaattcaat ggaggaaaaa taaatgggaa tgattggcta ttttgcagaa
      241 attgactcag aaaagattaa ccaactgctt gagtctactg aaaaaccttt gatggataat
      301 atacatgaca cgctttcagg attacgccga ctggatattg ataaacgctg ggatttttta
      361 cactttgggc tgacaggcac ctctgctttt gatcccgcga agaatgatcc gctgagtcgt
      421 gccgtgcttg atgaacacag cctcgaagat ggcatagatg gcttcctggg attgacctgg
      481 aatcaggaat tagccgcgac aatcgaccga ctggaatcac tggatcgaag tgagctgcgt
      541 aaacagtttt ccattaagcg actcaatgaa atggagattt atccgggagt gaccttttca
      601 gaagagttag aggggcaact ctttgcctcg atcatgcttg atatggaaaa gctgattagc
      661 gcgtatcgac gtatgctgcg acaagggaat catgcgctga cggttattgt tgggtgatgg
      721 tgtcagagcg ccaaaaaaaa ccgccgaaca tgtcggcggc tttctgactt acaaactaac
      781 agtttacatc agcggcattg ctaaccgcac aatactaatc agcggttgtg gccatacacc
      841 cagcaccagt accaacagtg cagaaatcag caccacgata ccgcccgcgc tgtactgcca
      901 gtttgacggt gcatcgcgac ccggttgttc cggtgcgtgc aggtacaggc tcaccgccac
      961 gcgcaggtag tagtagaggc cgattgccga accgacaacc acggcaccca ccagccacca
     1021 caagtgtgcc tggacaccga ccgccagcac gtagaactta ccgataaagc ccagcgtcat
     1081 cgggataccg gccagagaca gcatcatcac cgtcatcact gccgcgagga tcggacgatg
     1141 ccagaacaaa ccgcggtaag agaacaggga atcagcatcc gggccacgat acgggctgga
     1201 catcaggctg accacgccga acgcgccgag gctgctgaac agataaccgg ccaggtaaac
     1261 ccctaccgct tccatcgaca tctcgccggt ttgcagcgca atcagcgcca ccagcagata
     1321 gccgaggtga gagatagatg agtaaccgag cagacgtttg atgttggtct ggctcaacgc
     1381 catcaggtta ccgaagatga tggaggcaaa ggcgataatc gccagtacta cgcgaatcgc
     1441 ttcgctgtca cccaccggtg cgtagaggaa cagacgcatc accacaccga agatagcgat
     1501 tttgctcgcc gtcgccagga aagtggaaac cggcgcaggc gcgccctggt atacgtctgg
     1561 cgtccacagg tggaacggca ccagagagag tttgaagccg aggccaacaa tcatcatgcc
     1621 gaaacctgcc agcaacagcg gctcgttgag cataccgtcg ccgaggtttt tacccagcgc
     1681 gacaaacgac aggtcgccag actgcgcata caccagcgcc ataccgaaca gcaggaaaga
     1741 agacgctgcg gcagaaagga tggtgtattt gatactggct tccagcgaac gtttctggcg
     1801 gaaagcgtaa ccgaccaggc caaacagcgg caaagagatc agttcaatac cgaggaacag
     1861 agacgccaga tggtttgcat tcgccagcag gatcccgccc agcgcggcaa ttaacaccaa
     1921 caggtagaac tcatccttgt tgtcgttata gccttcaagc cacgggtagg cgaaagtaca
     1981 ggtggcgagg ctcgccaaca ataccagccc ggtgtaaagc atggcgaaac catcaacgcg
     2041 catcagcggc gtaacgtcca tagcgcccgc ctggccaaca aaccagagcg aaaccagcgc
     2101 cgcgttaagc ccaataaccg agagcgtagc gttgaggaaa tgattgcgtc gccacgcaat
     2161 ggagagcatc acaaccacca ccgtcaagcc gacgatcagc aacggtagca gtgcgatcag
     2221 gttttgtgga gttattgtca tggcgattta cggccttgta gtagtaacgg aattaacaaa
     2281 ccactgctgg atattgccaa tcgcggagtg cgaggtatcc agaatcggct gcggatagaa
     2341 gcccagcagt accagcagca ccaccagcaa caggatcata aacagctcac gcagcgacat
     2401 ccctggcagt tcctggctgg caatctggct tttcgcttta ccgaagtaag cgcgatgcaa
     2461 catcgccagc gaataaacag atgcaaagac cagcccaaag gtagagataa cggtaattac
     2521 cgggacaacc tggaagctgc cgaacagaat cataaattcg ccgacgaagt taccggtgcc
     2581 aggcatccca agcgttgcca ccgcaaagaa cagcgacagt gctggcagcc atttcatctt
     2641 gctccacaga ccgcccatca tgcgcatgtc gcgggtatgg atacgttcat aaagctgacc
     2701 gcagagaata aacagacccg ccgccgacaa gccgtgcgca atcatctgga ttaccgcgcc
     2761 ctggtaggcc aactggctgc cggtgtagat tgcaatcagc acgaagccca tgtgggaaac
     2821 cgaggtgtag gcgatcagac gtttgatatc ggtctgagcg aaggtcatcc acgcaccgta
     2881 gaagatgccg ataacaccca gccacatagc gattggcgcg aactctgccg acgcgttcgg
     2941 gaacagcggc agggagaaac gcaacaaacc gtaagcggcg gttttcagca agatccccgc
     3001 gaggtccacg gaaccggcgg tcggagcctg ggagtgcgca tccggcagcc agccatgcag
     3061 cggaaccacc ggcattttga ctgcgaaggc gatgaagaag cccagcatca acagatattc
     3121 cacaccactg gacattggcg tattcagcag ctcttcatag ttgaaggtcc agacgccggt
     3181 cgcattgtag tgaacaaaaa ccagcgccag gatggcgatc aacatcacca gaccactcgc
     3241 ctgggtgtaa atgaagaact tggttgccgc cgtgatacgc gttttaccgt cagaggcttt
     3301 atgcccccac agtgcgatca ggaagtacat cggcaccagc atcatttccc agaagaagaa
     3361 gaacaggaac atgtcgatgg caaggaacac gccgataacg ccgcccagga tccacatcag
     3421 gttgaggtgg aagaagccct gatatttttc gatctctttc cacgaacata gtaccgccag
     3481 cacaccgagc agaccggtca gcacgaccat cagcagcgac agaccgtcaa tggcgagatg
     3541 aatagagata ccaaaacgcg ggatccacgg catgtcgaat tcagactgcc actgcggaat
     3601 tccggcggat tgcgtcagtg aataaccgcc ctgcaaccac agttgcagcg acagcgccag
     3661 cgtcaatccc atggtgatca gcgcgatcca gcgtggcacc ttgacgccaa agcgttcggt
     3721 ctgccagcac aggaagccgc caataaaggg aattaatatt agccagggta gtaacatggc
     3781 gatctttatt ccttgtaaaa gtcccgtcag gaccggattt tcaacgaatt ctcacgacag
     3841 aaaattctct tcggactggg ggtagtgtcg gatgcgccgc gaaggcagct tatccgacct
     3901 acgttcttat atgcccaggg gcaatcccac aatccttaac tcaacgcagt accatcaaca
     3961 gtgccagcac cacgaccgca ccgatgctca tggatgccac ataccagcgc agataaccgt
     4021 tctcgcttaa cagcagacct ttacctgcaa agcgggaaag aaccgccggg atgttcatca
     4081 ttgagttcag cggatcgcgt ttcagcaacc aggcaatacc caggaacggc ttgacgaaca
     4141 ctttgtcgta cagccagtca aagccccagg cgttgtacca ccaggtactc agcaggcggc
     4201 ccggcgcact gttggcgatg gaggtcacca gagtacgttt acccagccac agccaggctg
     4261 ccagcagaat gccgaccacc gcgaccacgc cagaggtaat ttccagggtc aacatgctgc
     4321 cgtgcgccag ttccgtcgtt tgcggaagca cgccctgcag cggcggtaca atcagtgcgc
     4381 caacgaaggt ggaaaggatc agcagcacaa tcagcggcag gctgtgagtt acccctttca
     4441 cggcgtgagc gtgaatttgt tcttttccgt ggaagacgat gaaaatcata cggaaggtgt
     4501 agagcgaggt cataaacgca ccgaccagac ctgccaccat cagattgata tgaccattcg
     4561 ccatcgcacc cgcgaggatc tcatccttac tgaagaagcc cgcagtgacc agcggcagtg
     4621 ccgacagtgc tgcgccgccc accaggaagc agagataaac cagcggaata gatttacgca
     4681 gaccgcccat cttgaagatg ttctgttcgt gatggcaggc cagaatgacg gaaccggatg
     4741 ccaggaacag cagcgcttta aagaacgcgt gagtcatcaa gtggaaaatc gccgcatccc
     4801 atgcctgcac gccaagcgcg aggaacatgt agccaatctg gctcatggta gagtaagcga
     4861 gaacacgttt gatgtcggtc tgtaccagcg cggcaaaacc ggccagcagc agcgtaaccg
     4921 ccccgacaat acccaccaga tgcagaactt ccggcgtcat caggaacagg ccgtgggtac
     4981 gggcgatcag gtagacaccc gcggttacca tcgttgcggc gtggatcagc gcggagacag
     5041 gcgtcgggcc cgccatcgcg tcagcaagcc atgtctgcaa cggcaactgc gcagatttac
     5101 cgaccgcacc gcccagcagc atcagcgtcg cccacatcag catgttattg ccgtcagcaa
     5161 agtgcgctgg tgccagttcc accatttcgc ggaagttcag ggtgcccagt tcgttgtaaa
     5221 gaatgaacag tgcgaaagcg aggaaaacgt cacccacacg ggtcacgacg aacgctttca
     5281 ttgccgctgc gccattcttc ggatcggtgt aatagaaacc gatcagcaga taggagcaca
     5341 ggcccacgcc ttcccagccg aggtacatca gcagcaggtt atcggcgaga accagaacta
     5401 ccatgctggc gatgaacagg ttggtgtagg cgaagaagcg agagtagccc tcttcaccgc
     5461 gcatgtacca ggaggcgtac atgtgaataa ggaaacccac accagtgacc accgagagca
     5521 tggtcagcga caggccgtcc agcaccaggt taaaaccgat gttaaagtcg cctaccgaca
     5581 tccacgtcca cagcggctgg ctgtatgcct gctcgccgtt agcgaagaaa tcaacgccga
     5641 taaaggcggt taccagcgcc gccaggccca cagagccaac gccgacgatc gccgaaacgt
     5701 tttcagacca gcgcccacgg gagaacgcca gcaggacgaa gccaatcaat ggcaaaataa
     5761 tggttaaggc aagcatgttc atccgcgcat ctcacttact gaatcgatgt tcaggttctg
     5821 gcgacgacgg tgaagttgca gcagcagcgc aaggccgata ctcgcttctg ccgccgcgag
     5881 gctgatggcg agaatgtaca tcacctgacc gtcggtctga ccccagtagc ttccggcgac
     5941 cacaaaggcc agcgcggagg cgttaatcat gatttccaga ccaatcagca taaacagcag
     6001 attgcgacgg ataaccagac cggttaagcc aagaacgaat aagattgccg cgaggatcag
     6061 tccatgttgt aaggggatca tgcgtgctcc tccgtttttc ttttcgcgct gtcgtcttta
     6121 cgattgctca gcacttcacc cgcacgctct tcacgaccga cgtggaaggc cacaaccaga
     6181 cccgcgagca gcagcataga agccagttcc accgccagta cgtaaggccc gaacagcgta
     6241 atacccactg ctttagcact gattggcgta ccgtcgatac cctgatcgtt aacaccgagg
     6301 atggcgtaaa caatcaccac cagcatgatg gccgacaaaa ttgccgggcc aatccacacc
     6361 tgcggtttca gccactggcg ttcctgttcg atttctgaac cgcccaagtt gagcatcatc
     6421 accacgaaca cgaacagcac cataatggca cccgcgtaga cgataatttc cagcgcaccg
     6481 gcgaagtaag cgcctagtga gaagaacacc ccggaaatcg ccagcagcga aataatcagg
     6541 tacagcagtg cgtgtaccgg attggtatgg gtgatcactc gcaaggtcgc aagtatggct
     6601 atcaggccac agatataaaa agcgaactcc attgcccctc tccttacggt aacaggctct
     6661 tgacgtcgat aggcttggct tcgttctctg cttcgccctt atctttgccg tcgattgcca
     6721 tacctgccat ccggtagaag ttatattccg ggtatttgcc cggaccggag atcagcagat
     6781 cctctttctc gtaaaccaga tcctggcgct tgtattcccc catttcgaaa tccggggtta
     6841 actgaatcgc cgtggtcgga caggcttctt cgcacagacc acagaaaatg cagcgtgaga
     6901 agttaatgcg gaaaaattcc ggataccagc gaccgtcttt ggtttctgct ttttgcagcg
     6961 agatacagcc gaccgggcag gctaccgcac agaggttaca ggctacgcaa cgctcttcgc
     7021 cgtccgggtc gcgggtcaga acgatacgac cacgataacg gggcggcaga tagaccggct
     7081 cttccgggta cattcgcgtt tcgcgtttgg cgaacgcgtg caggccgatc atccagatac
     7141 tacgaacctg ggtgccgaaa cctactaaca attcttttaa ggtcatggtc ttattgcccc
     7201 ttattgcgcc tgccagagaa tgacagccgc cgttaccagc aagttgatca gcgtcagcgg
     7261 caggcagatt ttccagccga aggacattac ctggtcataa cgcggacgcg gtaacgacgc
     7321 acgaatcaaa atgaacatca tcataaagaa cgcggttttc agcgcgaacc agatgaatgg
     7381 cggtaacaac gggccttgcc agccaccgaa gaacagcgtc accatcaatg cagagatggt
     7441 cacaatcccg atgtactcac ccacgaagaa cagaccgaac ttcataccgg aatattcaat
     7501 gtggtaacca tccgccagtt cctgctcggc ttccggctgg tcaaacgggt gacggtgaca
     7561 caccgccacg cccgcgatgg caaaggtaat aaaaccaaag aattgcggga tgacgttcca
     7621 cacatgcgcc tggctgttga cgatgtcggt catgttgaat gaaccggcct gcgccaccac
     7681 gcccatcagg gaaagcccga ggaacacttc gtagctcagg gtctgcgcag aagcacgcat
     7741 cgcacccagc agcgagtatt tgttgttact tgaccagccc gcaaacagca ccgcgtaaac
     7801 cgccagacct gccatcatca ggaagaacaa aatcccgatg ttcaggtcgg caaccaccca
     7861 acccggactg actggcacaa ttgcaaaggc cagcagcagc gaggtaaagg caatcatcgg
     7921 tgccagggta aagatgacgc gatccgagaa tttcgggatc cagtcttctt taaagaacat
     7981 tttgatcatg tccgcaacca gctggagcga accgccccag ccaacacggt taggtccata
     8041 acggttctgg aacagaccca gcaggcgacg ttcgccaaag ctcatgaatg ccccacaggt
     8101 gacaaccacc agcaggatca ccaccgcttt gaggatggtc agcaggatct caatcagttc
     8161 cggtgatatc caactcattg ttgtgcctcc ttgagatcct caagatgcgc gccagccagc
     8221 accggagcaa tgccggacat acccatcggc aagcccacct gccctgccgt cagtccttcg
     8281 gcgatttcaa ccggcagcgt gaccgtgttg ccatcgtaac taaaggagac gcgtgtacct
     8341 gcgttcacac ccaacttcgc ggcatccgct gggttgagct tgatgtacgg ctgcggcata
     8401 cggctctgga agaccggagc acgctgtgac aattcatcgc tgccaaacag gtggtagtac
     8461 ggcgcgatac gccatttccc gtcctgcggc tggaagcgtg ccggtacgct ggtgaagtaa
     8521 tccagaccgt tttcgctggt ttcaaacaga cgcacgcccg gatcgccaaa gcgcagtttg
     8581 ccgcccactt cgtcctggaa tttgttccac gcctgcgggg agttccagcc cggcgcccag
     8641 gcaaacggca cttgcgaacg gtgcgcggtc ggctggttgt taccttccat cgagaaggtg
     8701 aacatggtgt caatatcctg cggctgacgc ggctcatgaa cgctgatatt ggcgcgcatg
     8761 gcggtacgac cgctgtaacg gtgcggttca cgggccagtt tctgcccacg aatacggaat
     8821 gtcgcatccg gcgcagcatc tttgataccc gccagttccg ggattttcgc cacaacagcg
     8881 tcaattacat ggtcgagctg cgtccagtcc acttcacggc tcagcaaggt gctgtgcagc
     8941 gagtgcagcc agcgccagct ttccagcatg acagttttgc tgtcgtaata agccggatcg
     9001 taaacctgga agaaacgttg ggcgcgacct tcattattaa ttaccgtacc gtcgctttca
     9061 gcaaagctgg cagcggaaag caccagatgg gcgttttcca tgatcgctgt gcgttgatga
     9121 tcaaccacca tcaccagcgg cgctttagcc agcgcagcat tcacgcgggt agcagaagcg
     9181 taacgatgca gatcgttttc caacaccacc accgcgtcgg cgcgtccggt ttccagttcg
     9241 gttaacgctt cttcaagcga accgccaccc ataatgccca gccccatgct gttgacggaa
     9301 cgggcaatca tggtgatacc aacgtcagcg ccgcgacctt tcagggcttt cgcgacgtta
     9361 gccgcagcct gaatcacctc tatgctaccg gcgttcgtcc cggagataat caacggtttc
     9421 ttcgcacctg ccagtgcctg cacgatgacg tcgattttgt tttgcagctc aggttcgata
     9481 ccgtcaaccg ctggtgcgga gttatccagc gcatgggcga tggcaaaacc taaacgcgcc
     9541 tgatcttcaa ccggtgcgcg gtaagtccac gccgcgatat catccagacg agtgtcatca
     9601 acgttggtaa caaacagcgg atgcttcgca cgttgaccga tgttgaggat tgccgcaatc
     9661 tgccagtcag ccactttctg tgctgcagcc atttcgcgcg ctttaccttt caccgcctga
     9721 cgcactgcca gcgcgacgcg cgcgccggtc tgggtaacgt cttcgcccag caccagtacc
     9781 gcatcgtaag attcgatttc gcgcagagcc ggagtataaa tgccgccttc acgcagcact
     9841 ttcagcgcca gttgcagacg ttcctgctca ccgtgagcga taccggtgta gaagttttct
     9901 tcgcccacca gttcacgcag cgcaaagttg ctttccacgc tggcacgcgg agaaccaata
     9961 ccgatcactt tcttcgactg acgcagaata tctgccgcgc cctgcattgc ctgttcggcg
    10021 ttgagggtaa tgaaatcatc gccacgacgc tgtactggct gacgcggacg atccttcagg
    10081 ttgacgtaac cgtaaccgaa acgaccacgg tcgcagagga agtagtggtt taccgtaccg
    10141 ttgtaacggt tttcgatacg acgcagttcg ccgtaacgtt caccggggct gatgttacag
    10201 ccgatggaac attgctggca gatgctcggc gcaaactgca tatcccattt acggttgtaa
    10261 cgctcggagt gcgttttgtc ggtaaatacg ccggtcgggc aaatttcgac caggttaccg
    10321 gaaaattcgc tttccagcgt gccgtcttcc gggcgaccga agtagacgtt gtcgtgcgcg
    10381 ccataaacgc ccagatctgt accgtcagcg taatctttgt agtaacgcac acagcggtag
    10441 caggcgatgc agcggttcat ttcgtgagag atgaatggcc ccaaatcctg attacggtgg
    10501 gtacgtttgg tgaaacggta gcgacggaag ctgtgtccgg tcatcacggt catatcctga
    10561 agatggcagt taccgccttc ttcacatacc ggacagtcgt gcgggtggtt ggtcattaac
    10621 cactcgacca cactttcacg gaactgtttc gcttcttcgt cgtcaatgga aataaaggtg
    10681 ccatcggaag ccggtgtcat acaggacatc accaggcgac cacgcgtgtc ttccgcgttt
    10741 tggtattgct tcaccgcaca ctggcggcaa gcaccgacgc ttcccagcgc cggatgccag
    10801 caaaagtaag gaatatcaag gcccagagac agacaagctt ccagcaggtt gtccgctccg
    10861 ttgacctcgt attctttgcc gtctacatga attgtagcca ttagcatgct tccagttttc
    10921 tcggtcaggg accgagcgtt aatcgaaatt cggttaccag cgctctttca gcaggttcgg
    10981 ctgaatccca ttaatcaaat gggtattgct gaacggctgt ttgattcccg cctcaaattc
    11041 ttcgcggaaa tatttgatgg cgctctgtaa cggctccact gcaccaggtg cgtgggcaca
    11101 gaaagtttta cccgggccta agaatcgaca cagttgctca agtgtttcga tatcgcccgg
    11161 ctggccttcg ccacgctcca gcgcacgcag aattttcacg ctccacggca gaccgtcgcg
    11221 gcacggcgta caccagccgc aggactcacg ggcgaaaaac tcttccaggt tacgcaccag
    11281 cgacaccatg ttgatctcat ggtcaaccgc catcgccagc gccgtaccca gacggctgcc
    11341 cgctttaccg atactttcga attccatcgg cagatcaagg tgcgcttcgg tcaggaagtc
    11401 agtccccgcg ccgcctggct gccaggcttt aaatttcaga ccatcacgca taccaccggc
    11461 gtaatcttcg aggatctcgc gtgcggtggt gccgaacggc agttcccaca gtcccggatt
    11521 tttcacccga ccggagaagc ccatcagctt ggtgccagca tctttacttt tcgagatgtt
    11581 ctgataccac tccacgccgt tagcgaggat cgccggaacg ttacacaggg tttcgacgtt
    11641 gttgacacag gtcggtttgc cccatacgcc ggaggttgcc gggaaggggg gtttcgagcg
    11701 tgggttagca cgacgccctt ccagggagtt gattaacgct gtttcttccc cgcagatgta
    11761 gcgccctgcc ccggtatgga cgaacagttc gaagtcgaaa cctgttccca taatgttttt
    11821 gccaagcaag cccgcttcag tggcttcggc aatggcgcgg cgcagattta ctgccgcttc
    11881 gatatattcg ccacgcagga agatgtagcc acggtaagct ttcagcgcaa acgcggagat
    11941 gagcatacct tccaccagca ggtgcggcag ttgctccatc aacaggcggt ctttataggt
    12001 gcccggctcc atttcatcgg cgttacacag caggtaacgg atgttcatgg attcgtcttt
    12061 cggcatcagg ctccacttca ggccagtcga aaagcccgcg ccgccgcgcc ctttcagacc
    12121 agcgtctttt acctgattaa cgatttcgtc cggagacagc ccggtcagcg ccttacgcgc
    12181 cccttcgtaa ccgtttttgc tgcggtattc gtccagccac actggctgtt tgtcatcgcg
    12241 cagacgccag gtcagcggat gcgtttcggg agtacggata atgtttttca tttataccgc
    12301 tccagcagtt cagggatcgc ttccggggtc agatgcgcgt gagtgtcctc atcgatcatc
    12361 atgtttggcc ctttatcaca gttccccagg cagcaagttg gcagcagcgt aaagcggcca
    12421 tcaaatgtcg tttgccctgg tttgatgttc agctttttct cgagcgccgc ctgaataccc
    12481 tgatacccgt tgatatgaca gaccacgctg tcgcaataac ggatcacatg gcgaccaacc
    12541 ggctggcgga agatctgact gtagaacgtt gccacacctt cgacgtcgct tgccggaata
    12601 cccagcacat cggcgatcgc gtggatcgca ccatccggca cccagccacg ctgcttctga
    12661 acgattttca gcgcttcaat ggacgccgca cgcgggtctt cgtagtggtg catctcgtgc
    12721 tcaatcgctt cacgctctgc cgcactcagc tcaaaagcct cggtttgtgg ttgttgattc
    12781 tcgtgcataa ttagcggtcc acatctgaca taacaaaatc gatactgccc agataaacaa
    12841 tcaggtcaga caccaggctg ccgcggatcg ccgtcggaat ttgctgcaaa tgcgcataac
    12901 tcggcgtgcg gatacgagtg cgataactca tggtgctacc gtcgctggtc aggtagtaac
    12961 tgttgatccc cttggtcgcc tcaatcatct ggaaagattc attggcaggc atcaccggcc
    13021 cccacgacac ttgcaggaag tgggtgatca gggtttcgat atgttgcagc gtgcgctctt
    13081 tcggcggcgg cgtggtcagc gggtgatccg ctttgaacgg gccttccggc atgttgttga
    13141 ggcactgctc aagaatgcgc agactctggc gcagctcttc cactttcagc attacgcggg
    13201 tgtagcagtc agaaacacca ccaccaaccg ggatttcaaa gtcgaagttt tcatagccag
    13261 aataaggacg cgccttacgc acgtcgaagt cgatcccggt agcacgcagg cccgcgccag
    13321 tggtgcccca ctccagcgcc tctttcgcgc cataggcggc aacgccctgg gaacgacctt
    13381 tcagaatggt attttgcagc gccgctttct cgtaagacgc cagacgtttc ggcatccagt
    13441 cgaggaactc acgcagcaga cgatcccagc cgcgcggcag gtcgtgcgct acgccgccaa
    13501 tacggaacca cgccgggtgc atacggaaac ccgtgatcgc ttccaccaga tcgtaaattt
    13561 tctgacgatc ggtaaaggcg aagaacacgg gcgtcattgc gccgacgtcc tgaataaagg
    13621 tcgagatgta cagcaggtgg ctgttgatac ggaacagttc ggagagcata acgcgaatga
    13681 cgttaacgcg atccggcacg gtgatcccgg ccagtttctc taccgccagc acgtaaggca
    13741 tttcgttaac gcagccgccg aggtattcaa tacggtcggt atacggaatg tagctgtgcc
    13801 aggactggcg ctcgcccatt ttctccgcac cacggtggtg gtagccgatg tctggtacgc
    13861 agtcgacaat ctcttcgccg tcgagttgta aaacgatacg gaaagccccg tgcgccgacg
    13921 ggtggttcgg accgaggttg aggaacatga agtcctcgtt ttcggtgccg cgcttcatcc
    13981 cccactcttc cggtttgaag gtcagggctt ccatctccag atcctgtttg gctttggtca
    14041 gctcaaacgg cgagaattcg gtagcgcgcg ccgggtaatc tttacgcagc gggtgacctt
    14101 tccaggtttg cggcatcatg atgcggcgca ggttcgggtg accgtcgaaa gtaatgccaa
    14161 acagatccca ggtttcacgc tcataccagt tagcgttcgg gaacagtttg gtgaaggtcg
    14221 gtacgtgcag gtcgttttct gccagcgcca ccttcagcat gatgtcgcgg ttacgatcga
    14281 tagaaatcag atggtagaaa acggaaaaat ccgcggcagg taacccttcg cggtgtgtgc
    14341 gcagacgttc gtccatgccg tgtaagtcaa acagcatgac gtaaggtttc ggcagtttct
    14401 ttaagaaatc gccaacttcc agtaattgtt cacgcttgat ccacacaacg ggaaccccgg
    14461 tgcgagtcgc ctgaacagta aaggcatccg gcccaaaacg gttgcgcagt tcgccaatta
    14521 ccggatcatc aagatgatcg cgggtctgcc aggcgggttc ttgcgcggtt aagtcggtca
    14581 tattgttcac cattgcaaat ggtccgtggt gactgtcggc gcagtaagct tcgcgaaata
    14641 gatttaggaa taagcgaaga aaaatccctt tgccgacagg cgcaaattaa atctcgtcag
    14701 gtgtacgcag gttggttacg gcaatgcgtt caccgcgctt gcgttcgcgc tctgattgca
    14761 tattggcgcg ataaacgccc tgatcgccaa ccacccagga gagcggacga cgttctttgc
    14821 cgatagattc ctgcaacagc atcagtgcct gcatgtacgc ttcaggacgc ggcgggcagc
    14881 ccgggatata cacatcaacc gggatgaatt tatcgacgcc ctgcacaacg gaataaatat
    14941 cgtacatacc accagagttg gcacaggcac ccattgagat aacccatttt ggttccagca
    15001 tctggtcata cagacgctga ataaccggtg ccattttggt aaagcaggtt cctgcaacca
    15061 ccatcaggtc agcctgacgc ggcgaagcac gcaatacttc tgcgccaaaa cgcgccacgt
    15121 catgcaccgc ggtaaacgaa gtcaccatct caacgtaaca gcaggaaaga ccgaagttat
    15181 acggccaaat tgagttttta cgaccccagt taaccatgtc attgagcttg cccataaaca
    15241 cgtttttgtt aacttcttgc tccagagggt cggttacgat ctcctgcttt tgcagggggt
    15301 aacggtcgtt ctcaccgttg ggatctatgc gggtgagcgt ataatccatc ttaatgcctc
    15361 gcggttagcg ttgacgatta gcgatactgt tcgtttccgg gttcatacgc tcgcggcgtg
    15421 aacgcgcggg cgtccagtcc agcgcgccaa tacgcaccag ataaaccaga cctgccagta
    15481 acacaaaaat aaaaattgca gcttccacaa agcctaccca gccgctttcg cggatagagg
    15541 ttgaccatgc gaacagatac agcgcttcaa cgtcgaagat aacgaagaac atggccacca
    15601 gataaaactt ggcggacagg cgtaagcggg cggagccgac cgagtcgata ccggattcaa
    15661 acggcacgtt tttcgacctc gcgcgtgcgc gaccgcctaa aaaccaaccg cctaccagca
    15721 tcaggcaaca caggccaatg gcaacgataa gaaagatagc gaatgcccag tgatgagcga
    15781 tgacttcagt ggatgttgac atactcattg cttactcatc aaaagtagcg ccagattcac
    15841 tgctcttcac ggcagatgga cgccacatcg attcatgggg aggaataaaa aaaaccttac
    15901 aatcactgta gaaattcttt tatacagcta attgatgtgg tcttttactc ctttctataa
    15961 ccttttgtca actttaacaa aagtttcttc acattagttt acataatatc aacaccatta
    16021 gcatttaatg ccctttcacc ccagatcctt gacgactcca ggataattag atgttgttga
    16081 atcgtgtccg ttgtgaagca atggaaaaaa tacgggtcta ttttgacagg aatttgtgtc
    16141 gattcctccc cccaaaagag agtattttct tgatctgtga cacgcttttg ttattccata
    16201 acaaaaacgc agcaacaaat ttacgtattt tttaacatca ttgtagcagg tgattttttt
    16261 caggcgatta tttgtgcgtt cgggacgtga atctctggtg attgaaaaat aaacagtttt
    16321 gtacgttctg cactatgcga ggaaggattt ttactaaaaa aaagccgctg gggtttaaaa
    16381 cacccccagc ggctcgtttt ttacactatt gtctcaggaa ttatctatcg tccgtcgatt
    16441 actcgatatc cctttcaatc aacaatgaat catccccttc cggagcagac attgggctgt
    16501 attgccacgg attatggtag ctctccatcg cctgatagat cacctgggcc agctcattat
    16561 tactggacgg gtcatagcac agcaaatatt cggtatcagg tagcggcggt aagccatcta
    16621 ctccactcag cacacgcagg tccgggctca tcatctcaac cggccttgcc gtcacgccaa
    16681 gaccggattt cactgccgca cgaacggccg gaagcgtcga ggcgacataa gccagtcgcc
    16741 atggaatatc tgctttatta agcgtcgcca gcaccatatc gcgaaacgga cttggatcat
    16801 ccagcaatac aagagggatc ggctcgcctt tttgcaatac gtattccgct gcgcagtacc
    16861 agtgtgttgg cgaggtacgc aggttcaatg ccttaaacgc tgaaggacga tgggtggtta
    16921 ccatcaaatc cacttcctgg gattccagca tttctgccat ataggcatta cgtttaacgc
    16981 ggacatccag cgctaatttc ggataaaccg aactcacgcg atttaacagg aaaggtaaga
    17041 tcgtatcggc agattcatct gaagcaccga tagttaacac gccctgaaga ttactgaaca
    17101 ttaatgatga gcaggcctca tcattaaaac gcaggatttt cctggcgtaa ccaagaagtt
    17161 gaatgccatg ttcagttaac agtttgttgc gaccgtgacg agcgaacagt tctttcccaa
    17221 cgagttgttc cagacgctgc atttgctgac ttacggcgga ctgagtacga cacacagcgg
    17281 cagctgcggc agcaaaagtg ttcagatcgg caacagcaac aaatgttctc agcagatcga
    17341 ggtcgaggtt aattatcgga cgatttgcac ttatcatata ttatcactta ctggcggctc
    17401 atactgagct ggttaatgct gtgcacacac aaacaagcaa ttccatttgt aatgtgcctc
    17461 cctggcagtt tcatccggaa cccggacgaa agtaaaaatg catatgagtt gcactaaaaa
    17521 agcgactcac attgttccgt tataatgcct gaagtagatc acagaatata tcttcaggga
    17581 tcgcatatct attaagttac tcactctttt ctatttatga catgcgcgtg tttgtataaa
    17641 tgtaaatgtg agtccttgtt ccactctcgt gcagcatcgc tggtcatacg cgaacacgta
    17701 caaacagcaa tggtgaagcg gcatcaagag cagggatccg tcatttatgc gagcatttta
    17761 ccccaaaaac cttttattta taagggtcat tgcgaattat ctgatgcaaa gttatgttat
    17821 gttaggcaaa gtaatcttct attattaata agcacatcaa aacctttttg aatattaaat
    17881 aataattaat tagcatcatc ctcattcatt aattccgctt aacaatagtt tcacacctta
    17941 cccccattgc gacacctcgg aaatcatcac gctgttaaac tctccgtaat gcggaaatat
    18001 catcatcgcg tttcattagt gaattcttaa atgaggcatt ttcacacaat tatcttacag
    18061 ataaaaaaac cagacttgca attaagaatt agaacaattc actatataac attgcatgta
    18121 aagcatatac acctcattat tttgtcatta ttaagttatt aacagcacaa tcgagccttc
    18181 ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg agtacattgt
    18241 tctaagctga cttccacggc agggagtggc gataacagca aaaaaggtca agattcatgt
    18301 cccccattga aaaatccagc aaattagaga atgtctgtta tgacatccgt ggtccggtgc
    18361 tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt actgaaactg aacatcggca
    18421 acccagcccc attcggtttt gacgcgccag atgaaatcct cgttgacgtg atacgcaacc
    18481 tgcctaccgc tcaagggtat tgcgattcca aagggcttta ctccgcgcgt aaagccatca
    18541 tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt tacatcggca
    18601 atggtgtatc ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc ggggacgaaa
    18661 tgttggttcc tgcaccagat tacccactct ggaccgcggc ggtttcgctt tccagcggta
    18721 aagcggtgca ttatctttgc gatgaatcct ctgactggtt cccggacctc gatgatattc
    18781 gcgctaaaat tacgcctcgt acgcgtggga ttgttattat caacccaaat aacccaaccg
    18841 gcgcggtata ttccaaagag cttttaatgg agattgtgga gattgcacgt cagcataatc
    18901 tcattatctt cgccgatgaa atttatgaca aaattctcta cgacgatgct gagcatcact
    18961 caattgcgcc gctggcacct gacctgctga ccattacctt taacggactg tcgaaaacgt
    19021 accgcgttgc aggcttccgt caggggtgga tggtgttgaa cgggccgaaa aaacacgcca
    19081 aaggctacat cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct aacgttcctg
    19141 cgcaacacgc cattcagacc gcgctgggtg gttatcagag catcagtgaa tttattaccc
    19201 ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga actgatcaac gatattccgg
    19261 gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc gacgccaaac
    19321 gctttaacat tcatgacgat cagaaaatgg tgctggattt cctgttgcag gaaaaagttc
    19381 tgttggtgca agggacggca ttcaactggc cgtggccgga tcacttccgc attgtcacgc
    19441 taccgcgtgt cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc ctttctggtt
    19501 atcatcagct gtaatcttaa tttcactgcc ggagattgca tccggcagtg ttatcccgcc
    19561 acaatgacct gatgatgtca tcatacgtaa ggtcactatg aaacagagcc atttttttgc
    19621 ccatctctcc cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg tgcggacgga
    19681 aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg cagctatcaa
    19741 aaatcggaaa tttggcggta atgtcaacgc cgaacgtatc gctttactgg cgatgtacca
    19801 cgatgcctca gaagtgctca ccggcgatct ccctactccg gtgaaatact tcaattcgca
    19861 aatcgctcag gagtacaagg ctattgaaaa aatcgctcag cagaaactgg tcgatatggt
    19921 tccggaagaa ctgcgggata tctttgcgcc gttaattgac gagcatgcat atagcgatga
    19981 agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca tatctgaaat gtctggaaga
    20041 actcgcggcc ggaaataatg aattcttgct ggcaaaaacg cgactggaag cgacgcttga
    20101 agcgcgtcgc agccaggaga tggactactt catggaagta tttgttccca gcttccatct
    20161 ttcgctcgat gagattagcc aggattcacc gctgtaagca gccggagtct gcgtcgcatc
    20221 aggcaataag cgccggatgc gacatcaggc tcttgtcaaa acggaaacag catcgggatc
    20281 atcaccacac aaaccgccat cacgataatg gtgaacggta cccccaactt cacaaagtca
    20341 ctgaagctgt aatttcccgg acctaaaacc agggtgttaa caggtgaaga caccggtgtc
    20401 ataaaggcgg cggatgctgc catcgccacg accatcgcaa atggataagg cgacactccc
    20461 atcgttttgg cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc ggtattagag
    20521 ataaacagcc caataaccgc cgacaagaca aacaaacacc ccagcatcat atgtggccca
    20581 taaccgccgc caatatccat cagccctttc accgccagcg cgacacctcc cgttttctgt
    20641 aatgccacag caaacggcat catcccaacg atcaaaataa tgctcggcca gtgaatggat
    20701 ttataggcgc tttcagcatc tatacagcgg aatttcccca tcagcaggca ggcgatgata
    20761 gcggcaacag gattaggaat ttcatctgtc agcattaacg ccaccattag caccagacag
    20821 aaaatggcat ggggtgcctg gctgtgcgcg gtcgatgctt cactcacctc ttccggtaag
    20881 ttcagcgcta cgaagtcgcg gccctgtttg gccagcatac cgatcagttt ccagttaccc
    20941 acaaccagga tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc ttccagcgcc
    21001 acgccattgc gcttgagccc caccacattc agtccgtagc gggtacgaaa accaatttcg
    21061 cgcaccgatt taccaatcag ttctgactca ggaattaatg aaatctctgc catgcccaca
    21121 tcaagggcct ggtcagaaaa atactcgccg cgcagtacca tcggctccag caattgctca
    21181 ctacaaaatt gccggagatc gacatcagcc gcagacatat caataagcaa aacgtcacgc
    21241 gcgcgaaatt cagaaacccc attaacgttc acgataacgc gacgaaaacg ccgccagcgt
    21301 tcaacaccga tgacgttagc gccataacgc tcacgtaatt tgagatcatc cagccgttga
    21361 cctatcattg gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt cagtcgatat
    21421 tcacggataa gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg ggtctgggta
    21481 tcccctttca gcatgaaacg catcactaac atatacaaaa tacccagcac cagcacgacc
    21541 aggccaatag gtgttacgct aaagaaactg aagccgtgat agccttcacg cagcaattca
    21601 ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct aatcagcccg
    21661 gcaaaactta acggcatcat cagacgtgac ggcgaagttt gcatacgcat ggcaacgctt
    21721 aacaccacgg gaataaagat agcgacaacg ccggttgaac tcataaacgc gccaagcccc
    21781 gcgacggtca gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc cactttgacc
    21841 agccatgttc ccattacggt ggcaacaccg gtacggacca aaccatcgcc aataataaac
    21901 aaggcggcaa tcaggacaac gttaggatca gaaaagccgg aaaatacttc ggggaccgtc
    21961 agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc catacgcact
    22021 ctgcccgtcg caaacaagac gatggcaacg gccagtaatg aaagaaccca aatcaattca
    22081 ccgttcacaa cttatccttg ttaattgagg gggatgactt gattctgcca taaaaaagcc
    22141 ccgacgagac ggggctaaat catgatcagg tgtttcactg aataataaca tcgccatttg
    22201 gctgtttggt cacagtaatt tgctccagac tgtggaggac caaatcgacc tcattcaggc
    22261 gcggggtatc tgccggagcg ttaaccgcaa tgacatggca acccgccgcc aggccagaaa
    22321 gcacgccagc gggagcatct tccaccacca cacactcctg cggcgcaagc cccagcagct
    22381 gcgcacctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc tcagcggtta
    22441 caaacacctc tggtgcggga agccccgcta ttttatggcg cgctcgcgct accggcatgg
    22501 agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgattg agtaaggcga
    22561 ttgccccagg aagcgcggta ataccttcgg tttccgtggc ctcgatttgc tccagacgcg
    22621 taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc aaagaggtga
    22681 tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca tgacgtctgg
    22741 cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt ccatcaagat
    22801 caaacagaaa acctttgcac cgcacgcggg cctcctcagg cgttgataat ttgattaatt
    22861 tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag catgcgctga
    22921 tatttttccc acattggcgt ctgggcgtta aaaccatgag tcccagcgtc aaaatgggta
    22981 tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc ttcagtggcg
    23041 gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc ctgcaaatta
    23101 gaccaggaaa catgcaacgc atgatacatc tccataatgt cgatgatagt gcggcaggtt
    23161 tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc gtaaccacgc
    23221 tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag catagtcatc
    23281 atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat tgttgactcc
    23341 tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt tatatgcgta
    23401 atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga aggcgcgctt
    23461 aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca acacctgtcc
    23521 agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag ggggtaatgg
    23581 cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta aggcgaacag
    23641 tgcagtggca actgccggcc caagcagacc gcccagggca atctgccagc agagagtaaa
    23701 tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa cgcgattttc
    23761 gacaaagacc ggggcaaggc gtttttccag tggccacgtc tttgagtaat gctgtccgcg
    23821 gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc ttcacctcaa
    23881 cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga caacgttcaa
    23941 aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag gcctaagtag
    24001 tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa aattacgcaa
    24061 aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag tgcatgatgt
    24121 taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac gtttttttag
    24181 ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc tgaactgcgg
    24241 tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt acctttctgg
    24301 tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg acggcaataa
    24361 acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact ttatcgttaa
    24421 tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg gtcaccgtat
    24481 cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg ttattcaggg
    24541 tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga tcggtatcga
    24601 agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat ttgacaccgc
    24661 gttccaccag actatgccgg aagagtctta cctctacgcc ttgccgtaca acctgtacaa
    24721 agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg taacccagga
    24781 agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct gccacctggg
    24841 caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct ctatgggtct
    24901 gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc cggcgatcat
    24961 cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac tgctgaccaa
    25021 agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg ttgaagacaa
    25081 ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc gcctggcgaa
    25141 atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg tattcactgg
    25201 tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac tgggcgtgct
    25261 gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat ctggtttcat
    25321 caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac tggttatcgc
    25381 gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg gcggtgctgt
    25441 tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg tattattatg
    25501 ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt gatccgtgca
    25561 atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc gcgtaccggt
    25621 ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac cacgacggcc
    25681 gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca gaaagatgtg
    25741 ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga agtcgttctg
    25801 gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa ctacgaaatc
    25861 gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga caccccggaa
    25921 cagctgaaag agcgtatcga actgacccgc aacagcttcg gtggtgccaa aaacaccaac
    25981 atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg tcgtactcgc
    26041 ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa tgttgatccg
    26101 gcgaagctgc aagaatccag cccgctgccg gttctcggcg ctgtgccgtg gagctttgac
    26161 ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat catcaacgaa
    26221 ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag cattccgcac
    26281 atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg tcctgacgtg
    26341 ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct gctgctgact
    26401 ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt cgctaccggc
    26461 ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct gcagagcttc
    26521 aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga atacgttgct
    26581 aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg cagccgtcgt
    26641 ctgtctccgc cagcgttccg ttatcagctg actgaactgg cgcgcaaagc gggcaaacgt
    26701 atcgttctgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat ctgtgctgaa
    26761 cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg tgttgcagcg
    26821 tctcagggtg tagaactagg tgcaggcatt gaaatcgttg atccagaagt ggttcgcgaa
    26881 agctatgttg gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga aaccgttgcc
    26941 cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca ggatgaagtt
    27001 gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc gccgctgcag
    27061 ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat gctgctgccg
    27121 gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc tgaacagctg
    27181 gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga accgcgcgtt
    27241 gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga aaaagttcgc
    27301 gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg tccgctgcag
    27361 tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc tccggttgca
    27421 ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg gtaacaccac ctacaaagcg
    27481 gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat gcgcaaaccg
    27541 gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat cgcgctgact
    27601 gcgattcagt ctgcacagca gcagtaatct cgtcatcatc cacagctttg cgctgcggat
    27661 atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatttg cattaatcct
    27721 ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatctca ctttgcatac
    27781 ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac tgaatccaaa
    27841 ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt tgctatttta
    27901 accagtcttg ccacctgggt agttccggtg gggatgtttg acagtcagga agtgcagtat
    27961 caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt tcgcattctg
    28021 actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac gggcgatgaa
    28081 cgcccgggcc tgatgaactt cccgtttgaa gggttaacct caggatcgaa atacgggaca
    28141 gccgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat tgtgatgcgt
    28201 acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg gaatgaaatt
    28261 ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt tggtatggga
    28321 gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct gggctatgac
    28381 agtattacca ccgtcctggt gacttatatt gccacgcaaa tcggttttgc cagttcgtgg
    28441 atgaacccgt tttgtgtggt tgttgctcag gggattgccg gcgttccggt gctttctggc
    28501 tccgggttgc gcatcgtggt gtgggttatc gccactctga ttggcctgat ctttaccatg
    28561 gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga gtccgaccgc
    28621 ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg tgactggctg
    28681 gtattgattg tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat cgttaatgcc
    28741 tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat tggcatcatc
    28801 ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggattcatc ctttaccgaa
    28861 ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg gattttgctg
    28921 ctggttggta atggtgaagc aggtaatgcc agcgtgttaa atactatcct caacagcatt
    28981 gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt gctcttccag
    29041 gctgtattta atttcttcgt gacgtccggt tctggtcagg cggcgttaac tatgccgtta
    29101 ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct ggcgttccag
    29161 tttggtgatg gcttcagcca catcatttac ccaacctcag cttcgttaat ggcaacgctc
    29221 ggtgtgtgcc gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct gcttggactg
    29281 ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta ccactaaaaa
    29341 tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc tacggcttca
    29401 tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg agtgaactca
    29461 tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa cttcactgac ttcatcttcc
    29521 tgtagggcga agggaccgtg agagacgcaa ctgaacaacg cgccccagac acggcaattt
    29581 ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat gcccaactct
    29641 tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac tacaccgcct
    29701 gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg ctggaccaga
    29761 attttaccca tgccatcatg cacgacgatg taagttgcac gatgacgcag acactgtgcc
    29821 cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac aatatccacc
    29881 cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt ttctggcgct
    29941 cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg cgcaatgata
    30001 ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc ataactcgcc
    30061 ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat ctccccccgc
    30121 tgttctgcca ctggcaaatg ggtatgaccg tacaccagca catcgttctg gtttaaagca
    30181 ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg tttttccagt
    30241 aatacctgtt gccacggcgc ggttatcggg aaatgcagca tcatttgatc cacttcgctg
    30301 tcgcagttgc cacgcacagc gataacctta tgtgccactt cattaagccg ttcagcgact
    30361 ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa cacgtcgcca
    30421 aggatcacca gccactgggc accgctttgg acaaacaact ccacaacacg ttccgtcgcc
    30481 ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc ctcgtcgaaa
    30541 aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct tcgccgaaag
    30601 tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat ccaccaggcg
    30661 ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca gatcagtatc
    30721 agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat gttctgccat
    30781 cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt ttttcgcccc
    30841 cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc tgcgcagcca
    30901 ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa tacgttccca
    30961 ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt cactcaactc
    31021 aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt gccacgtcgg
    31081 ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca ggcctttttc
    31141 ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat ctgaccaaag
    31201 cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct atcaaactat
    31261 agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg ctttctatac
    31321 ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta tttcgccccg
    31381 acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga ttatcgcttg
    31441 attaaggtag acctggggaa aggcggtcag tttcgtccgg aatttttgcg catttcgccc
    31501 aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga accgttaagc
    31561 ctctttgagt ctggtgccat tttgttgtat ctggctgaga aaacaggact ctttttgagt
    31621 catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt aggcggactg
    31681 gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac cattccttac
    31741 gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa caagtggctg
    31801 gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc ctgctggccg
    31861 tgggttaatg cctggactcg cctgcgaatt gacctcgcaa tgtatccggc agtcaagaac
    31921 tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa agcacaactc
    31981 ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt catcctaagt
    32041 aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata aagaaccttc
    32101 gtttgcgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag gatattgtta
    32161 tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat atcaacgatg
    32221 cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat aaccgtttct
    32281 ctttgctgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat cactgggtga
    32341 cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat tcggtatcca
    32401 tgaccttaag ctggcagcgt taatcgccat atcgggaggc tgcatgaaca tagtgatcac
    32461 cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc tgggccatca
    32521 aattacggta gtgacgcgta acccgcagaa agccagttcc gttctcggcc ctcgggtgac
    32581 actatggcaa gggcttgccg atcaaagcaa cctcaacggc attgatgcgg taatcaacct
    32641 ggccggagaa ccgattgctg ataaacgctg gactcacaag caaaaagagc gtctctgcca
    32701 aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgccagcg acacgccgcc
    32761 gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg aagtggtggt
    32821 taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc gctgggaaga
    32881 gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa ccggcgtggt
    32941 actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc ttggcctggg
    33001 cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg atatggtcaa
    33061 cggcattctc tggctgctgg ataacgagct gcgcgggcca tttaatatgg tttcgcccta
    33121 cccggtacgc aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc gcccggctat
    33181 tttgcgcgtc cccacaaccg ccattcggct gttaatgggc gaatcttcag tactggtatt
    33241 aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt ttcgctggta
    33301 cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc aaacatccgc
    33361 cagttaactc cctgtgttac aggattagtg gctttgcgcg ataagatcgt ctggtgaaag
    33421 tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct cctgagcaat
    33481 acgcagggtt tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc tgtcagcaat
    33541 ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa taaatgcacg
    33601 gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta gctgtctgtc
    33661 attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca acagatcgcg
    33721 ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt gcataatctc
    33781 gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata ttttgcgggc
    33841 tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag gacttctgca
    33901 accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg catcaagatg
    33961 gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt cttccggctt
    34021 actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt tcacagacca
    34081 caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg gttctagttt
    34141 aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat caataaaatt
    34201 ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg gcgtggaagt
    34261 tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg acaataacca
    34321 cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc agaagtattt
    34381 ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc ccttgaaaat
    34441 actccggcag cgttagccac cggagaggaa attatttcag cgatccctta aggaactgtt
    34501 gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct tcttctattt
    34561 tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc atttcgtgag
    34621 tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt aacacttcgc
    34681 ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact tccggttcca
    34741 tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga tgcaccggat
    34801 atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc gcccgctcgc
    34861 gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg ttttccaaca
    34921 ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt gtgcgcagta
    34981 agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc accagattga
    35041 tcgtctggcc attgaccacg atcgatcctt cactcggttt ttcgaggaag ttaatgcagc
    35101 gcagaaaagt acttttcccc gatcccgacg atccgatgat gcttattaca tctccggcat
    35161 tcgcttgcag tgagacccct ttcagcactt cgtgttcgcc atagcgtttg tgcaaatcga
    35221 taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg tttcacatgc
    35281 tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga gatgattaaa
    35341 tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc gttaatatcg
    35401 cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt agagtgcagt
    35461 atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga aggcaaaata
    35521 atgcagcgat acattttaaa ggtcgagaag ccataggctc tggcggcttc aatttcccca
    35581 tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca ggtgttaagc
    35641 gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag gaattcggtt
    35701 cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg aacatacagc
    35761 ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta tttattactg
    35821 gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac cgacaaaata
    35881 agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca cagcagcggt
    35941 ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc acggagtagc
    36001 ggcgctcaag gaacagcagc acaccattgg aaacggtggt gaaaaccagg taaatcacgc
    36061 cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagc tgcgtggctt
    36121 tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg atcacctgcc
    36181 agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg atccgccgaa
    36241 acacttgccc gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct ttcggcactg
    36301 ccataaacgc gccacggaac gtttcggtaa aataagcacc gtagataaaa ccgagagtga
    36361 taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc tccgtcacca
    36421 tgtttagcgc aatctgcaaa ccatagaaaa tcagcagcat caacaccaag tccggcacgc
    36481 ctcgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt tgcgagagct
    36541 taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg atagccagct
    36601 ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac atgctgcctg
    36661 tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg aagggagcgg
    36721 gctttctttc accgatggcg attaaccacc ataaacatca aaatcgaagt actttttcgc
    36781 taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca gtgcctcgcg
    36841 cagttcgtta tcttctttac gcaggcccat gccggtccct acaccaaaca gtttttcatc
    36901 tttaacagac ggaccaccga atttgtaatc tttaccgacg ggttgtttga ggaaaccttc
    36961 gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca ggtcagaata
    37021 aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc agtgttcatt
    37081 accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt tcagcgactc
    37141 gactgtcggc tgaatgtcag aatttttcgc caccaccaga cgagaatcgg cagcgtacag
    37201 tttgtcggtg aaggcaattt cttgctgacg tttttccgta atggaaagcg atgacatgat
    37261 ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcgggt tttcgacaaa
    37321 cgtacattgc gtattgatgc gcttgcacaa ctccttcgcc agctcgatat cgaaaccaac
    37381 cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg taccgatgcg
    37441 gatgttttgc ggaattgcag caagtgccgc agttgcgctg gagaaggcca gaaccagaga
    37501 gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta tagggaatat
    37561 ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa atatcccgcc
    37621 ccgttagact tattattttt cattaaaaat gcttaagtat gcatttcaat gcaccacgat
    37681 agtgcaacaa acctagcgtg caccatgatg atcattccat caggtacagc ttcccagcga
    37741 cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg tcgtaggtgc
    37801 cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca gcatcatctt
    37861 tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact gatgagccag
    37921 caaaggcgaa atctttacca gcaggttgct tgaggaatcc ttcgctagca gcaacttcat
    37981 cttgtaacgc agcatccaga cgccctgcag ccagatcgga atagaccaaa tcctggttgg
    38041 cataggccac cacatccacg cctttactac gccaggtctc gttagcgtaa gcttcctggg
    38101 ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt ggctgaatcg
    38161 gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg gagaaggcaa
    38221 tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg atttttttcg
    38281 ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ctaggtacat ttcacctgca
    38341 tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca cctttagcat
    38401 ctttcgacga gaacggcgca taggtggtat cggttccgat acgtaccgtc tccggtagcg
    38461 ccgcatagct ggaagccgct gcggagagac cgactaataa agacagagcg agaatcgact
    38521 tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg tgttgtgtgt
    38581 ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac attcaacgtt
    38641 aaatatgtta ataagacgtt gcattattgt cctgaagttg aagatagcag gtatagcggt
    38701 tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg atcgcggttg
    38761 caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg ggcaaagaga
    38821 tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg atttatcaca
    38881 tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat caccgcaccg
    38941 atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt ttcacgcacg
    39001 cagagtacca acgggcgacg ctctttcagc accacatctg ccgcacgggt cagtaagcca
    39061 tcagtatagc tatggacaat gccggaaagg gttttgattg aacagggtaa aatcaccatc
    39121 cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc atcgtgcgtg
    39181 acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga taaggtctgg
    39241 cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg cagaacctgt
    39301 aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat gagtcgtttc
    39361 ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc gggagttgca
    39421 agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat gcatttcgag
    39481 attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta acgtatcgag
    39541 gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga acaccgagca
    39601 ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat cgttcagatc
    39661 ctggaaaatc accccgtcag caccgatgat ctggcgaatt tcatcaactt cgcgaccgtg
    39721 agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga agcgaatttc
    39781 cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca tctcgataat
    39841 ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt ttttatcgcg
    39901 gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca gctgctggcc
    39961 cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc ggtacggttt
    40021 gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga tcgggatcac
    40081 cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt tcgtgcccat
    40141 attcacacgc gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc gggcaaagta
    40201 tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac gggtaaacaa
    40261 ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca ggaaatcaaa
    40321 gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac ggttctcgtc
    40381 aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga aagcaaccat
    40441 accgtggccg ataatcatcg ccacacaggc atacgcgccg cggattaagc ggtttgtggc
    40501 agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt tgtccagctc
    40561 gctggcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt ggcggcgttt
    40621 ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt tgtgggcaag
    40681 cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc tggagctgcc
    40741 agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct gcatatggcg
    40801 agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc agttattggc
    40861 atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg ttaaggcatc
    40921 ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc acatacgtct
    40981 ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac ttgacgagct
    41041 ttgcagataa tcaaaaaacc atctgatgat aaaactgaat tgcgggatca gctgtgattt
    41101 gctccagtct tcacttttcg acaccccggt aaaggagtcg agaaagaaga gaatggcggc
    41161 aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat cggtgcctga
    41221 caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac caacgatcag
    41281 ggtagcgata aacagtaccg cgatggcaat cccatttcga accagttcgt cttcaaagcc
    41341 cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag cacaacccca
    41401 tgtcaccagc gataacgctt cacgaacaaa gccgcggatc aggctaacca gagaggaaaa
    41461 agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac gattttacgc
    41521 cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg tagggatttc
    41581 cttcccgcgc atcaataaaa atggcgctga aaaaatattc aacgccatcg actttttatg
    41641 cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc cgacctacgg
    41701 ttctaacact gcccaagcct gataagacgc gccagcgtcg catcaggcaa gactgtatta
    41761 attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca actcacccag
    41821 cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg taattttacc
    41881 ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca gcttaccgac
    41941 aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat aggctttacc
    42001 cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg caggtggtgc
    42061 ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga ccggtgcagg
    42121 ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg acggtgctgc
    42181 ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg gcaacgcctg
    42241 ggtggcggct ggcatcatat caggctcatc acgatcgccc gctttcggca ccagcgggat
    42301 agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg gaagtacaat
    42361 cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact tacttgccac
    42421 cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa gaaccacaca
    42481 ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc tgcgcaacgc
    42541 tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg gcaccgcgcg
    42601 gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag gccagagttc
    42661 cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg tttttcggta
    42721 gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg acatcaaaaa
    42781 taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg ctggcaatcc
    42841 cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc gccagcgccg
    42901 ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg ccgtgagcat
    42961 cgctaaacac ccagtcatga tcggtgacgg aatagttcca ctcaacgccc cgacgttgta
    43021 acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc ggctcaccga
    43081 caattgccgg tttttcgctg cggaagatgc ctgctttctc gcgaccaata ctttcgcgat
    43141 ctggacccag ccagtcggta tgatccagcg caatactggt tactaccgcg acatcggcat
    43201 cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc accacgtcaa
    43261 gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa taggtcaggg
    43321 aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc gattccagca
    43381 attcctggcc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc gaactgtaga
    43441 cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag gtggtgcctt
    43501 tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg ccaagacgcg
    43561 ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc aggttttcca
    43621 gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg ataatcatgg
    43681 tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc cctgccctta
    43741 tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg ccttcacgcg
    43801 gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc gccagtttca
    43861 ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc aggaattcac
    43921 tgcgctggaa tctaggcggc agtttttcgc gaacggtctg ttcgataaca cgcggaccgg
    43981 caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg gcgaaacttg
    44041 cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag ccgcgctcct
    44101 gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc agtgcttcct
    44161 gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct tcctgcgcct
    44221 gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg cccataaagg
    44281 cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc atcaccacca
    44341 gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta tacttcttgg
    44401 agtcacgaaa cttcagctca tctttcggct caagctcgct acccagctcc acaaggcttc
    44461 cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga tggtcacact
    44521 tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga ccgcagctat
    44581 cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta atgttgcttt
    44641 taattcgttc aatccagctc attagggacc tttctgtctg aacctagttc gatgccagtt
    44701 ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat gttgcacatt
    44761 aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag ttacttttta
    44821 ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc ccggcaaaat
    44881 agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca tcgggattgt
    44941 accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc cgatcacgtt
    45001 ataagcagcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg gggcgaacgt
    45061 tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt tctcataaaa
    45121 ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat tactgaataa
    45181 cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa tcgcggcgat
    45241 cagcatcagc accaccatca tatggacgtt gagatcgttg gtttccagtg atgccaacgc
    45301 tccggcaaca aacagcaatg aatcacccgg taaaaacggc attaccacca gaccggtttc
    45361 acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg cgactagctc
    45421 tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca ggtccatatt
    45481 tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac agcctgaaat
    45541 gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc ggttttggaa
    45601 gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct tttgccgttg
    45661 ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc tccggctggt
    45721 tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga tgtacaaagg
    45781 cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta atatgcataa
    45841 cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg aaatcattct
    45901 cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt tcgtaaaaat
    45961 gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg atgtagcgat
    46021 aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct ttaacccaac
    46081 gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc gcgtctttgc
    46141 gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct gcgtcagtac
    46201 gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc ttttccagct
    46261 tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta ctgccgtcgt
    46321 actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac attagtacag
    46381 atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc caaagcgaac
    46441 gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat tacgcacgca
    46501 gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga attcgttctc
    46561 ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt ctgccgccag
    46621 cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg gtgcctggac
    46681 gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt cgtcaacgat
    46741 acaacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca tattgaacgc
    46801 cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca atttcgcact
    46861 ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa tcaggctggt
    46921 aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg ccagcagctg
    46981 gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg tcagtacaaa
    47041 cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca gtccgctgct
    47101 gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg tagcttcttt
    47161 gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg catcctgcac
    47221 ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac gtgccagtgc
    47281 ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg cttcgcccat
    47341 agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg tttactccag
    47401 aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga ttaacgtgcc
    47461 ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact ggcgtcatca
    47521 caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg cagtttatcg
    47581 aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg cacatcatac
    47641 accaaatgca ccagcctttt cagcgtcggt tgatcgagcg gaccatgcag cgtaatgcgg
    47701 ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg cccaataaac
    47761 ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag ggtatagcct
    47821 gcgatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag ttccggctcg
    47881 ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca ggtcagcagc
    47941 gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc gggcttcagg
    48001 ctacggatca gtttttcatc cgccagatgt agcgttttgt acggaccatc tttaaagagt
    48061 ggcgtatgga aagtcagaat atccgcgcgc tggactaact catccagcga gcggaaatca
    48121 ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat ccctaacgct
    48181 tccagtcgca cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc caccgtacgg
    48241 tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa aacatattcc
    48301 accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc ctgcttcaac
    48361 catgcttcat cgacatggtc ggtccccgca gtggcagttc caacaaattt aatgggtttt
    48421 cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc atccgcgtct
    48481 gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc caaacggcta
    48541 aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt tgtgttacct
    48601 gtatgagacg agagttaacc ggacaagtgt gccataatct cgcggccagg catacttgcg
    48661 aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc ctcctaggca
    48721 gccaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt tatccagtca
    48781 gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc aacaacaaag
    48841 agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt caccgctgct
    48901 ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg ccgcgcaaaa
    48961 aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc tggggaataa
    49021 tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc tgaatcagct
    49081 ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga tggttattcc
    49141 gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc acaatgcgct
    49201 aaaacagctg gtgaccaaac ttgcggcggc aacgggggaa cccagcaaac agatctggca
    49261 atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga aactgtttaa
    49321 ccatctggtg acctggctac aggcgcgtca gacgctaagc cagcaaaata cgccgacgct
    49381 ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg cggcgttatc
    49441 ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt ctgcccaggc
    49501 cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc cgcgtgatat
    49561 gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata tggcaacgcg
    49621 tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc tggttcgtta
    49681 accccgacga aatgacagta tcgtgacaat aatacccagc accgcagaga tcgccccggc
    49741 aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg gcccggagac
    49801 gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc gaacttgtga
    49861 ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac atccggctcc
    49921 ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg gcttgccaga gcagcaacaa
    49981 gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa aacggtccgg
    50041 catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg taagagtaaa
    50101 gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc cgataaccgc
    50161 aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc cgacaacgct
    50221 ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc cgttacaggc
    50281 ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac cgtaatggct
    50341 atgaatcaac aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg ccattccgtt
    50401 ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc aggtcagagc
    50461 gcctgtcagt aactggcttt caccaaaccc aagaatcaaa cgcccgatga ccaacagggc
    50521 aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc cgccagccag
    50581 accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat cggccagtcg
    50641 cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atcccgacgg caatgccgac
    50701 catggtattg ccatagccca gatcatgatg aacaaacagc gggataaccg gcaacggcaa
    50761 ccctacggtc atgtaggtga gaaaaaccgc aaaagcgatg cggaagagcg aaaaactggc
    50821 agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa tgcagcgcaa
    50881 ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt tggcaggatt
    50941 taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct ggaactggtg
    51001 gcgattgtca atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt agtgccggag
    51061 gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac ctactccggc
    51121 ctactgcgaa ttaatctttc agcttgcgca ttaccagcgt ggcgttggtg ccgccgaagc
    51181 cgaagctgtt agacataaca gtggtcagtt cgcgatcggt cgtttcggtc acgatgttca
    51241 gacccgcagc ctgctcgtcc agctcttcaa tgttgatgct cggggcgata aagccgtgtt
    51301 ccagcatcag cagagagtag atagcttcct gcacacccgc agcgcccagt gagtgaccgg
    51361 tcatggcttt ggttgcagaa atcgccgggc tcttatcgcc gaacacttca cggatagctg
    51421 ccagctcttt cacgtcgcca accggagtcg aagtaccgtg agagttcagg taatcgattg
    51481 gggtatcaac gccatgcatc gccatctgca tacaacgtac tgcgccttcg ccagacggag
    51541 caaccatgtc tgcaccatca gaggttgcgc cgtagccaac gatttcagca tagatgtaag
    51601 caccacgcgc cagcgcgtgt tccagctctt caaccactac cataccgccg ccgccagcga
    51661 taacgaaacc gtcacggtga gcgtcgtaag tacgggaggc tttttccggg gtgtcgttgt
    51721 atttagtaga cagcgcaccc atcgcgtcga attcgcaggc catttcccag cacagctctt
    51781 cgccgccgcc agcaaacacg atgtcctgtt tgcccagttg gatctgctct actgcgttac
    51841 cgatacagtg tgcggaagtc gcacacgcgg agctgatgga gtagttaacg ccatgaattt
    51901 taaacggcgt ggcgagacag gcagaaacgc cggatgccat cgctttagtg accacatacg
    51961 ggccaaccgc tttcaggccg cgcgggccgc gcattgcgtc agcgccgaac acctggaaac
    52021 gcggggaacc gccgccggaa cctgcaatca ggccaacgcg cgggttattc tggtaagctt
    52081 ccggagagag gcccgcatca gcgattgcct gctccataga aaggaatgca taaatggatg
    52141 cgtcgctcat aaagcgcaca actttgcggt caatgaggcc agtggtatcc agttttacgt
    52201 tgccccagac gtggctacgc atgccggaat ccttcagctc ctgagagaaa gtgatccctg
    52261 aacgtccttc acgcagagat gccaggactt cctgctggtt attaccgatg ctggaaacaa
    52321 tgcccaggcc agtaatcact gcacgtttca ttcaatacct ctgtaagtcg cacatagagt
    52381 aagtttcgaa tgcacaatag cgtacacttg tacgccgaac aagtccgatc agccatttaa
    52441 tagagaaatt tgcgcagcct tacacacatc gctaagatcg agccaccgcc tgtaagacga
    52501 gtaacttacg tgaaacacta ctccatacaa cctgccaacc tcgaatttaa tgctgagggt
    52561 acacctgttt cccgagattt tgacgatgtc tatttttcca acgataacgg gctggaagag
    52621 acgcgttatg tttttctggg aggtaaccaa ttagaggtac gctttcctga gcatccacat
    52681 cctctgtttg tggtagcaga gagcggcttc ggcaccggat taaacttcct gacgctatgg
    52741 caggcatttg atcagtttcg cgaagcgcat ccgcaagcgc aattacaacg cttacatttc
    52801 attagttttg agaaatttcc cctcacccgt gcggatttag ccttagcgca tcaacactgg
    52861 ccggaactgg ctccgtgggc agaacaactt caggcgcagt ggccaatgcc cttgcccggt
    52921 tgccatcgtt tattgctcga tgaaggccgc gtgacgctgg atttatggtt tggcgatatt
    52981 aacgaactga ccagccaact ggacgattcg ctaaatcaaa aagtagatgc ctggtttctg
    53041 gacggctttg cgccagctaa aaacccggat atgtggacgc aaaatctgtt taacgccatg
    53101 gcaaggttgg cgcgtccagg cggcacgctg gcgacattta cgtctgccgg ttttgtccgc
    53161 cgcggtttgc aggaagccgg attcacgatg caaaaacgta agggctttgg gcgcaaacgg
    53221 gaaatgcttt gtggggtgat ggaacagaca ttaccactcc cctgctccac gccgtggttt
    53281 aatcgtacgg gcagcagcaa acgggaagta gcgattatcg gcggtggtat tgccagcgcg
    53341 ttgttgtcgc tggcgttatt acggcgcggc tggcaggtag cgctttattg cgcggatgaa
    53401 gcccccgcgc tgggtgcttc cggcaatcgc cagggggcgc tgtatccgtt attaagcaaa
    53461 cacgatgagg cgctaaaccg ctttttctct aatgcgttta cttttgctcg tcggttttac
    53521 gaccaattac ccgttaaatt tgatcatgac tggtgcggcg tcacgcagtt aggctgggat
    53581 gagaaaagcc agcataaaat cgcacagatg ttgtcaatgg atttacccgc agaactggct
    53641 gtagccgttg aggcaaatgc ggttgaacaa attacgggcg ttgcgacaaa ttgcagcggc
    53701 attacttatc cgcaaggtgg ttggctgtgc ccagcagaac tgacccgtaa tgtgctggaa
    53761 ctggcgcaac agcagggttt gcagatttat tatcaatatc agttacagaa tttatcccgt
    53821 aaggatgact gttggttgtt gaattttgca ggagatcagc aagcaacaca cagcgtagtg
    53881 gtactggcga acgggcatca aatcagccgt ttcagccaaa cgtcgactct cccggtgtat
    53941 tcggttgccg ggcaggtcag ccatattccg acaacgccgg aactggcaga gctgaagcag
    54001 gtgctgtgct atgacggtta tctcacgcca caaaatccgg cgaatcaaca tcattgtatt
    54061 ggtgccagtt atcatcgcgg cagcgaagat acggcgtaca gtgaggacga tcagcagcag
    54121 aatcgccagc ggttgattga ttgtttcccg caggcacagt gggcaaaaga ggttgatgtc
    54181 agtgataaag aggcgcgctg cggtgtgcgt tgtgccaccc gcgatcatct gccaatggta
    54241 ggcaatgttc ccgattatga ggcaacactc gtggaatatg cgtcgttggc ggagcaaaaa
    54301 gatgaggcgg taagcgcgcc ggtttttgac gatctcttta tgtttgcggc tttaggttct
    54361 cgcggtttgt gttctgcccc gctgtgtgcc gagattctgg cggcgcagat gagcgacgaa
    54421 ccgattccga tggatgccag tacgctggcg gcgttaaacc cgaatcggtt atgggtgcgg
    54481 aaattgttga agggtaaagc ggttaaggcg gggtaatctg ctctcgcagt gtttgccgga
    54541 tgcggcgtga acgccttatc caacctacgt gtgacgtgta ggcctgataa gacgcgataa
    54601 gcgtcgcatc aggcatggtg ctccagacgc cgcaacttac agctgtgacg cctgctgaaa
    54661 caggttttcc cacatatcgg tcaccagcgc ctggtcacgc ggcgacaatt caccggcacc
    54721 aatggctttt tccagactct ggctaacggt cgtatgcacc gcctgagcgg agtggtcatc
    54781 accactttcc agttctgcga tggctaatgt caggtggcca cgcaaatacc cactggcaaa
    54841 caactcatca tcactggcat ggtcaaccat gccgtcgatt aatgccagaa tgcgtgattc
    54901 aaactccgcg atcatcttct ttcctcattg taaaacgtgg cgctggcttc agttgagcgc
    54961 ttccggccac gggaactgtt ccgccgtaag ttccggcgtg tgataataat tttgtagtgc
    55021 tttaatgaag cgtgccgggc gttccggaat gccatttgtc agataatcca tcacctgcgc
    55081 atgtacccgc cgttgaaaca ccacacgatc cggttcgaaa tccccttcca gattgtcgca
    55141 actgacgtta aacggatatc cagccgccac gcagaataac cagtcgaacg cctgcggctt
    55201 cacttcaaca tcttcaaact ggctttgcgt ctgggcatcg cgtccgtccg ggcaatacca
    55261 gtagccgaaa tcaaccagct cacggcgcgc ttttccggca atacaccagt gcgaaatctc
    55321 atgaattgcg ctggcataaa agccatgagc aaagacgatt cggttatacg gtacttccgc
    55381 atcagcagga agatagatcg gttcgtcgtc gcctttaatc agacgggtat taaattcatc
    55441 ggcaaagcag ctattaaaaa tttcaatcaa ctgctcgtag tggtgtgtac tgttcattag
    55501 ttcatcccca accagtggag gatctcctgt ccgtggctat cgtaaagtaa tttggcactc
    55561 atcaccgccg agacgataac aatcatcggg cggatcagct tttgtccttt gctcaacacc
    55621 aaccgtgaac ccatgcgcgc gccgaggaac tgccccacca gcatgacaaa gcccgtcgcc
    55681 caaatcactt tgccgccgag aataaacagc agcagaccgc caatgtttga cgttgcgttg
    55741 agtaatttgg cgtgagccgt ggctttagcg aggttgaatc cgcacagcgt aacgaaggcc
    55801 agcgcgtaaa acgatccggc agccgggcca aagaatccgt cataaaaacc gacgcagcca
    55861 ccggcaatta acgcgaaggg taaaccgtac atccggcgct ggcgatattc ttcacccagc
    55921 tttggcatca gcaagaaata gaggccgata caaatcacca gaatgggcaa aatctgccgt
    55981 aagacatcag cctgaacgta ctgtaccaac agtgcgccgc tcattgagcc gacaaaggtc
    56041 atggcaatat tgagtttctg atcgcttaaa ctaaccactt tgcggcgaat aaagtagata
    56101 gtggcggaaa tagagccgcc gcaggcttgc agtttattgg ttgccagcgc gttagcggga
    56161 gacattcccg cagccatcaa tgccggaatg gtgagtaacc caccgccacc ggcaatcgag
    56221 tcgataaatc ccgccagcat ggcgacaaaa aagagaactc ccagcaacag cggagaaacc
    56281 ataaacaggc tattaaacgt ttccattaga tcacgtgctc atccagtagc gcctggcagg
    56341 aaggcggcaa cggaggcggt gtcttcttct caggctttgt tgttcccggt tttggaggtt
    56401 caaaccagct ttgcagttct gccccgcaac catcgcctga tggcggtaaa ggttgatctt
    56461 cacactccag actatcggca gggcaacgta atcgtacatg catatgcgcg cgatgctgga
    56521 accagggtcg cactttgcgc aaccagtcgc gatcggtgcc cgcatcaagg caaagttgtt
    56581 gtttaatcgc cggattaaca aaaatgcgcg tgacgtcttt gtcctgggcg gcgagtttga
    56641 tcaagctgaa aatttctggc ttccacagcg tggagacaac gtgtttaccg tcgcgggaaa
    56701 ctaagtccag tgcttgcggg cgcaagagct gcgcggaggt ccagcgagtt ttcggcagtt
    56761 gcagaaagat atcgacatcc agtccggtct ggtggctggc atgaccgccg ttgaaacgcc
    56821 caccagcggg catccccata tcgccaatca gcaccgtacc catgcccaga ttgctcactt
    56881 ggctactcag acgctggata aacatcacca gatccgggtg accgaaatag cgacgctgat
    56941 cggtacgcat gacctgataa tgttcggact gtatcggcaa cgtgtcagcg ccgacaatac
    57001 agccattaga aaaactgcct atcgattgtg cgctacccgg cacaggttgg gttatttttt
    57061 gccacggcgt cgctgccagg ctagcgctac tggcaagcag agccagcagc gcaatcgcgg
    57121 ttttgttcat tttttaccag cgtggaatat cagtcttcac atcggcattt tgcgcccgct
    57181 gccgtaacag gtgatccatt aaaacgatcg ccagcatcgc ttcagcgatc ggcactgcac
    57241 ggatcccgac acagggatcg tgacggcctt tggtgatcat ctcaacttct tcgccaaagc
    57301 ggttaatagt gcgccccggc acggtaatgc tggaggtcgg tttcagcgcc atatgagcaa
    57361 tgatttgctg cccgctgctg ataccgccga gaatgccgcc cgcatggttg ctctggaaac
    57421 cgtctttggt gatttcgtcg cggttctggc tgccgcgcag cgccactacg tcaaaaccgt
    57481 cgccaatttc cacgcctttc accgcgttga tgctcatcag cgcatgggcg atgtcggcat
    57541 ccaggcggtc aaagaccggc tcgccaagtc cggcggggac accactggca acaacggtga
    57601 ctttcgcgcc gatggagtcg ccctcttttt tcagcgcgcg catcagttca tctaacgcgt
    57661 cgattttgtc ggggtccggg cagaagaacg gattttgttc aaccagcgac cagtctttga
    57721 tttccagcgg aatgtcgccc atctgggtca ggcagccgcg gatttcaata ccaaatttct
    57781 cggcgagata ttttttggca atcgcccctg ccgccacgcg catggcggtt tcgcgggcgg
    57841 aagaacgtcc accgccgcga taatcgcgca gaccgtattt ttgttcgtag gtgtaatcag
    57901 catggcctgg acggaagacg tctttaattg cgctgtaatc ctgagaacgc tggtcggtat
    57961 tttcgatcaa caagccaatg ctggtgccag tagtaacgcc ttcaaaaaca ccggagagta
    58021 ttttgacctg atccggctcg cggcgctggg tggtatagcg cgatgtccca gggcgacgac
    58081 gatcaaggtc atgttgcagg tccgcttccg tcagcggaat gcctggcgga acaccatcga
    58141 cgatgcagcc gagcgccagc ccgtgcgatt cgccgaaggt ggttacgcga aagagttgtc
    58201 caattgtgtt tccagccatc acggctccgt tattgttgtg tttgcgtgtt tacttaatct
    58261 ttataaatcg cgaaatgttc tcgtgcggca ataagctgct ctttagtgag cataaacaca
    58321 ccatcgccgc cgttatcaaa ctccagccag gtgaacggaa catcgggata ttgttccata
    58381 agatgtacca tgctgttgcc cacttcacaa atcaacacgc catcatcagc aaggtaatct
    58441 gccgcgttac cgagaatgcg acacgtcagt ttcaggccgt cggtgccaga tgccaggccc
    58501 agttccggct cgtgacggta ttcgtttggc aggtcggaca tatcttccgc gtcgacatac
    58561 ggcgggttag tgacaatcag atcgtattgc actttcggca aatcacggaa cagatcggaa
    58621 cgaatcggaa tgacattgtg gatcagaccg tgttcttcga tgttctgttc ggcaaccgcc
    58681 agcgcgtcgg gagagatatc taccgcgtcg acttccgctt ccgggaaggc ataagcacag
    58741 gcaatggcga tgcagccgct accggtacac atatctaaaa tatgctgcgg ttgcttgctg
    58801 atgagtccgg caaatttatt gttgatcagt tcaccaatcg gcgagcgcgg caccagcacg
    58861 cgttcatcga cgtaaaattc atgaccgcag aaccaggctt tgttggtcag gtaagccacc
    58921 ggaatgcgct cgttgacgcg gcggatcacg cgttcaacaa tacggtgttt ttcgctggag
    58981 gtcagacgcg cggtgcgcat atcttccggg atatccagcg gcaggtagag cgatggtaac
    59041 accagctgta cggcttcatc ccactggtta tcggtgccgt gaccgtacca gatatttgcc
    59101 gcgctgaagc ggcttaccga ccagcgcaac atgtcctgaa tggtttgcag ctcatttact
    59161 gcttcatcaa cgaaaatttt atccacgtat tcctccaggg catgatcgca ataatttcgg
    59221 cggctagttt gccatgaaga tgacgataaa tcagcattca cgcgcggtga gtgaggaaaa
    59281 atacgtttaa aacgatcgat tgtgctacga gtcgggtaaa ctgtaggaaa attagaaata
    59341 gagacagata aatgaaaaag aaaacaacac ttagcgagga ggaccaggct ctgtttcgcc
    59401 agttgatggc ggggactcgc aagattaagc aggacacgat tgtccaccga ccgcagcgta
    59461 aaaaaatcag cgaagtgccg gtgaaacgct tgatccagga gcaggctgat gccagccatt
    59521 atttctccga tgagtttcag ccgttattaa ataccgaagg tccggtgaaa tatgttcgcc
    59581 cagatgtcag ccattttgag gcgaagaaac tgcgccgcgg cgattattca ccggaattgt
    59641 ttttggattt acacggtctg acgcagctgc aggccaagca ggaactgggc gcgttgattg
    59701 ccgcctgtcg ccgcgaacat gtgttttgtg cctgcgtgat gcatgggcac gggaagcata
    59761 ttttgaagca acaaacaccg ctgtggctgg cacaacatcc gcatgtgatg gcatttcacc
    59821 aagcaccgaa agagtacggc ggtgatgcgg ctttgttggt gttgattgaa gtggaagagt
    59881 ggttgccgcc ggagttgccc tgaatgctgg cagcaaatgc cggagcagcg taaactcctt
    59941 atccggccta caaatatgtg ataattcaat aaattgcaat tcacttcgta ggcctgataa
    60001 gacgcatcag agcaatacta cgaacttcag ttcattcaat acatactgaa aacgacacgt
    60061 cgaatcaatc ggttgcccac tactggtatt cacattcacc acaatatcgt gtagtacttt
    60121 atccgttacc ggatgacgag ttggcgtaac cgttaccgtt gatttatcac aaccgtaaga
    60181 ggtaaaggtt aacaattctg ataccgcgcg gcagtatgct cctctgccat cattatcaga
    60241 aaaattactg ccatcacaga gtctgccgcc ttccatcagg taggtcgaaa gcgtggtggt
    60301 cactgttgac cccgtggact gaattcgtaa cgccttatta tggctgacat aattttcact
    60361 ccaggagata tcgtatgaag cgaccggtat cgttgggcat attgtgttat gcactgtagg
    60421 ggccgtatag cttccaaatc cgggtaaatc tgtataggaa taaaaggatg cgatccaagg
    60481 gcaaccataa tcatcagatg caacggtaat tttaaacgtc atatatgcac aacgaaaaat
    60541 tttcgtatct atgccagggc aatttgagtt attaattgta tcaaagtcag tcagtaccaa
    60601 agttggagct gaacccgacc cagaccagga aataacccgc aacgtatcgc ctttatacgc
    60661 aacagaatca tatttcccat atgcaacctc ctttggcgtt atcaccgtaa cattgaaaac
    60721 actgctcgct acagcaccgg ttaccttgtc catggcataa taaactctca cactgtcaaa
    60781 actcgcactc ataagtttat ctccagcata actcttagat gcgccgacaa cactcagaac
    60841 gagcagcatt aggctcgcga aaattatggt ccattttttc atttcagaaa tactcaaagg
    60901 ttgccgtcac tgtggcacta aagtctccgg acgttacatc tcgcccgctc tttgcctgta
    60961 accaggtatt aaaatttagg ctgttatttc cctgattcag cgggagcgtg acgcctgttg
    61021 tattgttgac tggcaccgat gtgcctgccg cagtttcaat accaatccca gcgccggatg
    61081 ctgatgacga ggagtcaaag gccagaaagc cgggtagagc actatcttct gttcctgtca
    61141 acgttacttt gacattaaac aatgtagaac tatgacaatc tttcaattga aacaccaatg
    61201 gcactcgctc tgactgccca aggttcatta aatcgctggc ggcaatggca ggaaaatgta
    61261 cttctgccaa ctggctacca ttaatcactg gcgtacacga tttactgatt aaattgccgg
    61321 taaagtgcaa attatctcct gctattgtgg ttccactgcc cagcaacatc aggctactaa
    61381 taaaaaacaa cgtacgcttt ttcttcattg gtaatctacc tgtaacgtgg cccaggcctc
    61441 aaagtccgcc tctggcagaa cgacaccact cttttttact ggcacagccg ttaacaccgg
    61501 taaatcggtt tcattcacca caagtggcgt attgatcgta aaactctgcc ctgcctgttt
    61561 gagctgaatg cctaaaccag tgatattgct gcttactgca gcattatcaa aatcgcttgg
    61621 tgtgccactc cagctcaagg tcatttccat taatgggtcg cgaaccgccg tatcgcaggt
    61681 aatggaatac ggcacggttt gctcataatt gctgccatta atgtcatcaa taagcacatc
    61741 ctgaaaagtt atttcaatgg tttgagcgtt gctaatcgtg cagttaggcg gctgcactaa
    61801 ataaccgtga aaagagacgt ttgagagtgc catcgagcaa aagccccaca gcaaaataag
    61861 cgagatccgt ttcattggta atccaccaca attgtggcac tggcgttaaa ttcagctgcc
    61921 gctaacgttg taccactctg tttcaccggc acagcttcca gaacgggaac tcccgaggaa
    61981 aaattaaatg gcaaccatga gccagaggtt aagtcgagta ttgtgtgatc actacttttc
    62041 tgaacccgaa taccaaaccc aggaataccc gtgctgataa ctgtttcacc gttaatcacg
    62101 accgttgtcg cctgcatctg tagacgcagg tcatccattg ccagattgtt acaaacaagc
    62161 gagtagtcaa tgggacgttt ataatcaacc ccattgatct tattaatgaa tacattacca
    62221 aactcaactg ccgcaccatt taccgtacaa ggaggagggg catcgacaag cacccgtaaa
    62281 ttcatactta ctgacttact cgctgcaact gcaaagccgc taccaacaca acatatcagt
    62341 aacccagtta ccgcccagac agaacaagat cctttcatac tggctctccc ggcctcagcc
    62401 tttgcggctc tgctcttcat c
//
LOCUS       DBKEKI010000006        61793 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.006, whole genome shotgun sequence.
ACCESSION   DBKEKI010000006 DBKEKI010000000
VERSION     DBKEKI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.006"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..434)
                     /locus_tag="ACUQPX_000362"
     CDS             complement(<1..434)
                     /locus_tag="ACUQPX_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HHV9055164.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTCSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGAPIK"
     gene            752..1393
                     /gene="udk"
                     /locus_tag="ACUQPX_000363"
     CDS             752..1393
                     /gene="udk"
                     /locus_tag="ACUQPX_000363"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HHV9055165.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            1485..2066
                     /gene="dcd"
                     /locus_tag="ACUQPX_000364"
     CDS             1485..2066
                     /gene="dcd"
                     /locus_tag="ACUQPX_000364"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HHV9055166.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            2088..3941
                     /gene="asmA"
                     /locus_tag="ACUQPX_000365"
     CDS             2088..3941
                     /gene="asmA"
                     /locus_tag="ACUQPX_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HHV9055167.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4393..5976)
                     /locus_tag="ACUQPX_000366"
     CDS             complement(4393..5976)
                     /locus_tag="ACUQPX_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HHV9055168.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFSFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(6177..6326)
                     /locus_tag="ACUQPX_000367"
     CDS             complement(6177..6326)
                     /locus_tag="ACUQPX_000367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055169.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            6635..7774
                     /gene="wza"
                     /locus_tag="ACUQPX_000368"
     CDS             6635..7774
                     /gene="wza"
                     /locus_tag="ACUQPX_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HHV9055170.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7780..8223
                     /gene="wzb"
                     /locus_tag="ACUQPX_000369"
     CDS             7780..8223
                     /gene="wzb"
                     /locus_tag="ACUQPX_000369"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HHV9055171.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            8226..10388
                     /gene="wzc"
                     /locus_tag="ACUQPX_000370"
     CDS             8226..10388
                     /gene="wzc"
                     /locus_tag="ACUQPX_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HHV9055172.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHTRPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGGSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            10480..11319
                     /gene="wcaA"
                     /locus_tag="ACUQPX_000371"
     CDS             10480..11319
                     /gene="wcaA"
                     /locus_tag="ACUQPX_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HHV9055173.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            11322..11810
                     /gene="wcaB"
                     /locus_tag="ACUQPX_000372"
     CDS             11322..11810
                     /gene="wcaB"
                     /locus_tag="ACUQPX_000372"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HHV9055174.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            11807..13024
                     /gene="wcaC"
                     /locus_tag="ACUQPX_000373"
     CDS             11807..13024
                     /gene="wcaC"
                     /locus_tag="ACUQPX_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HHV9055175.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            12999..14216
                     /gene="wcaD"
                     /locus_tag="ACUQPX_000374"
     CDS             12999..14216
                     /gene="wcaD"
                     /locus_tag="ACUQPX_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HHV9055176.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMEPISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEVIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFSDNRNFRVQLYLFTPVSLFFTGSIFSSEYAFLIV
                     CPFILRKALNITR"
     gene            14227..14973
                     /gene="wcaE"
                     /locus_tag="ACUQPX_000375"
     CDS             14227..14973
                     /gene="wcaE"
                     /locus_tag="ACUQPX_000375"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HHV9055177.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLVHLAQADDISFEWIVVDGGSN
                     DATREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            14989..15537
                     /gene="wcaF"
                     /locus_tag="ACUQPX_000376"
     CDS             14989..15537
                     /gene="wcaF"
                     /locus_tag="ACUQPX_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HHV9055178.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVALGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            15563..16684
                     /gene="gmd"
                     /locus_tag="ACUQPX_000377"
     CDS             15563..16684
                     /gene="gmd"
                     /locus_tag="ACUQPX_000377"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HHV9055179.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            16687..17655
                     /gene="fcl"
                     /locus_tag="ACUQPX_000378"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             16687..17655
                     /gene="fcl"
                     /locus_tag="ACUQPX_000378"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0050577 - GDP-L-fucose synthase activity
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HHV9055180.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPASLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            17655..18134
                     /gene="gmm"
                     /locus_tag="ACUQPX_000379"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             17655..18134
                     /gene="gmm"
                     /locus_tag="ACUQPX_000379"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HHV9055181.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            18131..19354
                     /gene="wcaI"
                     /locus_tag="ACUQPX_000380"
     CDS             18131..19354
                     /gene="wcaI"
                     /locus_tag="ACUQPX_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HHV9055182.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            19357..20793
                     /gene="cpsB"
                     /locus_tag="ACUQPX_000381"
     CDS             19357..20793
                     /gene="cpsB"
                     /locus_tag="ACUQPX_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HHV9055183.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            20986..22356
                     /gene="cpsG"
                     /locus_tag="ACUQPX_000382"
                     /gene_synonym="manB"
     CDS             20986..22356
                     /gene="cpsG"
                     /locus_tag="ACUQPX_000382"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HHV9055184.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            22411..23805
                     /gene="wcaJ"
                     /locus_tag="ACUQPX_000383"
     CDS             22411..23805
                     /gene="wcaJ"
                     /locus_tag="ACUQPX_000383"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HHV9055185.1"
                     /translation="MINLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            23807..25285
                     /gene="wzxC"
                     /locus_tag="ACUQPX_000384"
     CDS             23807..25285
                     /gene="wzxC"
                     /locus_tag="ACUQPX_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HHV9055186.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIIHYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            25465..26745
                     /gene="wcaK"
                     /locus_tag="ACUQPX_000385"
     CDS             25465..26745
                     /gene="wcaK"
                     /locus_tag="ACUQPX_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HHV9055187.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELIVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            26742..27962
                     /gene="wcaL"
                     /locus_tag="ACUQPX_000386"
     CDS             26742..27962
                     /gene="wcaL"
                     /locus_tag="ACUQPX_000386"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HHV9055188.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            27973..29367
                     /gene="wcaM"
                     /locus_tag="ACUQPX_000387"
     CDS             27973..29367
                     /gene="wcaM"
                     /locus_tag="ACUQPX_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HHV9055189.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            29542..30435
                     /gene="galF"
                     /locus_tag="ACUQPX_000388"
     CDS             29542..30435
                     /gene="galF"
                     /locus_tag="ACUQPX_000388"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HHV9055190.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            30808..31893
                     /gene="rfbB"
                     /locus_tag="ACUQPX_000389"
     CDS             30808..31893
                     /gene="rfbB"
                     /locus_tag="ACUQPX_000389"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HHV9055191.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            31893..32792
                     /gene="rfbD"
                     /locus_tag="ACUQPX_000390"
     CDS             31893..32792
                     /gene="rfbD"
                     /locus_tag="ACUQPX_000390"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HHV9055192.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            32851..33729
                     /gene="rfbA"
                     /locus_tag="ACUQPX_000391"
     CDS             32851..33729
                     /gene="rfbA"
                     /locus_tag="ACUQPX_000391"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HHV9055193.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAGQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            33734..34279
                     /gene="rfbC"
                     /locus_tag="ACUQPX_000392"
     CDS             33734..34279
                     /gene="rfbC"
                     /locus_tag="ACUQPX_000392"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HHV9055194.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWQVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            34295..35551
                     /gene="wzx"
                     /locus_tag="ACUQPX_000393"
     CDS             34295..35551
                     /gene="wzx"
                     /locus_tag="ACUQPX_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HHV9055195.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            35581..36471
                     /locus_tag="ACUQPX_000394"
     CDS             35581..36471
                     /locus_tag="ACUQPX_000394"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HHV9055196.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNSTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGDGRYNA"
     gene            36464..37369
                     /gene="rfbG"
                     /locus_tag="ACUQPX_000395"
     CDS             36464..37369
                     /gene="rfbG"
                     /locus_tag="ACUQPX_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HHV9055197.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            37389..38537
                     /gene="wzy"
                     /locus_tag="ACUQPX_000396"
     CDS             37389..38537
                     /gene="wzy"
                     /locus_tag="ACUQPX_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HHV9055198.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            38541..38915
                     /locus_tag="ACUQPX_000397"
     CDS             38541..38915
                     /locus_tag="ACUQPX_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HHV9055199.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            38978..39904
                     /locus_tag="ACUQPX_000398"
                     /pseudo
     CDS             38978..39904
                     /locus_tag="ACUQPX_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603067.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            39933..40688
                     /locus_tag="ACUQPX_000399"
     CDS             39933..40688
                     /locus_tag="ACUQPX_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055200.1"
                     /translation="MIELPLYLYVVYCFAFGMTFFVIGNIVLSSFFKEGVAGLNKFSL
                     FFYCLCIGMSLSILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEF
                     STLFVILFLVLIFAVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFP
                     QNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYLGIIATILLFTI
                     NPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            40702..41340
                     /locus_tag="ACUQPX_000400"
     CDS             40702..41340
                     /locus_tag="ACUQPX_000400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055201.1"
                     /translation="MIFLCGFFIGIACGVKLFGAIYGAIIFVILIAKKRNIQTFLIFS
                     LATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQTAEQARHGISPATLD
                     LQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWFSVTQGDRYLSPIYP
                     LSVLLVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILNSNIFILNA"
     gene            41382..41696
                     /locus_tag="ACUQPX_000401"
     CDS             41382..41696
                     /locus_tag="ACUQPX_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055202.1"
                     /translation="MKKASEYSELYGDNLIQLGLEDGIYFYKGMAIGDVFGLARYSDW
                     TISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYPKFKVLMDTPNGILIA
                     IK"
     gene            41805..43211
                     /gene="gndA"
                     /locus_tag="ACUQPX_000402"
     CDS             41805..43211
                     /gene="gndA"
                     /locus_tag="ACUQPX_000402"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HHV9055203.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYNVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            43461..44627
                     /gene="ugd"
                     /locus_tag="ACUQPX_000403"
     CDS             43461..44627
                     /gene="ugd"
                     /locus_tag="ACUQPX_000403"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HHV9055204.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            44773..45750
                     /gene="wzzB"
                     /locus_tag="ACUQPX_000404"
     CDS             44773..45750
                     /gene="wzzB"
                     /locus_tag="ACUQPX_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HHV9055205.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(45936..46547)
                     /gene="hisIE"
                     /locus_tag="ACUQPX_000405"
     CDS             complement(45936..46547)
                     /gene="hisIE"
                     /locus_tag="ACUQPX_000405"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HHV9055206.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANSIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPEISYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(46541..47317)
                     /gene="hisF"
                     /locus_tag="ACUQPX_000406"
     CDS             complement(46541..47317)
                     /gene="hisF"
                     /locus_tag="ACUQPX_000406"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HHV9055207.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            complement(47299..48036)
                     /gene="hisA"
                     /locus_tag="ACUQPX_000407"
     CDS             complement(47299..48036)
                     /gene="hisA"
                     /locus_tag="ACUQPX_000407"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HHV9055208.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            complement(48036..48626)
                     /gene="hisH"
                     /locus_tag="ACUQPX_000408"
     CDS             complement(48036..48626)
                     /gene="hisH"
                     /locus_tag="ACUQPX_000408"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HHV9055209.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRHSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            complement(48626..49693)
                     /gene="hisB"
                     /locus_tag="ACUQPX_000409"
     CDS             complement(48626..49693)
                     /gene="hisB"
                     /locus_tag="ACUQPX_000409"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HHV9055210.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            complement(49693..50763)
                     /gene="hisC"
                     /locus_tag="ACUQPX_000410"
     CDS             complement(49693..50763)
                     /gene="hisC"
                     /locus_tag="ACUQPX_000410"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HHV9055211.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRPLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            complement(50760..52064)
                     /gene="hisD"
                     /locus_tag="ACUQPX_000411"
     CDS             complement(50760..52064)
                     /gene="hisD"
                     /locus_tag="ACUQPX_000411"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HHV9055212.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYVAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            complement(52070..52969)
                     /gene="hisG"
                     /locus_tag="ACUQPX_000412"
     CDS             complement(52070..52969)
                     /gene="hisG"
                     /locus_tag="ACUQPX_000412"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HHV9055213.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     misc_feature    complement(53016..53139)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(53115..53165)
                     /gene="hisL"
                     /locus_tag="ACUQPX_000413"
     CDS             complement(53115..53165)
                     /gene="hisL"
                     /locus_tag="ACUQPX_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HHV9055214.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            53448..53699
                     /gene="yefM"
                     /locus_tag="ACUQPX_000414"
     CDS             53448..53699
                     /gene="yefM"
                     /locus_tag="ACUQPX_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HHV9055215.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            53696..53950
                     /gene="yoeB"
                     /locus_tag="ACUQPX_000415"
     CDS             53696..53950
                     /gene="yoeB"
                     /locus_tag="ACUQPX_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HHV9055216.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            54033..54857
                     /locus_tag="ACUQPX_000416"
     CDS             54033..54857
                     /locus_tag="ACUQPX_000416"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHV9055217.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGRIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            54903..55832
                     /locus_tag="ACUQPX_000417"
     CDS             54903..55832
                     /locus_tag="ACUQPX_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HHV9055218.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            56047..56109
                     /gene="yoeI"
                     /locus_tag="ACUQPX_000418"
     CDS             56047..56109
                     /gene="yoeI"
                     /locus_tag="ACUQPX_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HHV9055219.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            56099..57457
                     /gene="plaP"
                     /locus_tag="ACUQPX_000419"
     CDS             56099..57457
                     /gene="plaP"
                     /locus_tag="ACUQPX_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HHV9055220.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            57636..58694
                     /gene="tsuA"
                     /locus_tag="ACUQPX_000420"
     CDS             57636..58694
                     /gene="tsuA"
                     /locus_tag="ACUQPX_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HHV9055221.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            58708..58935
                     /gene="tsuB"
                     /locus_tag="ACUQPX_000421"
     CDS             58708..58935
                     /gene="tsuB"
                     /locus_tag="ACUQPX_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HHV9055222.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(58978..60402)
                     /gene="sbcB"
                     /locus_tag="ACUQPX_000422"
     CDS             complement(58978..60402)
                     /gene="sbcB"
                     /locus_tag="ACUQPX_000422"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HHV9055223.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            60584..61762
                     /locus_tag="ACUQPX_000423"
     CDS             60584..61762
                     /locus_tag="ACUQPX_000423"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9055224.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGERVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
BASE COUNT        15689 a        14350 c        15727 g        16027 t
ORIGIN      
        1 aacttaatag gggcaccccc caacagaggc ggaacaatgg cgctgccgaa tgccagacgc
       61 agccagtcag ccagattttg caaggggtta taccacggca gcaatttacg tagcagcact
      121 gccccgacca cggcttcaac aatattgatg gtcgtccagg tcatgttcag cgagcaggtg
      181 gaaaaaagca ggatggatgc ggcgatattt cccagcgaac agctcagcgc aattcccggc
      241 cacatgcgcc cggcatggcg ataaaacgcc accatcatga tggacgtcgg gaaccacagt
      301 ggggcgagtt gggtgccaaa ctgcgaaagc tcaagcgaga caagggtaaa gataaacgag
      361 actaaaccta aactgaccag gtgaagcagc gggtggggca gggcaattaa tacatgctgt
      421 gattgtttgc ttattaccgc tttatccgca aggccaggtc gccagtattc gtttctgtgt
      481 ttatgctagt acaaaagttt tacattttat atgcgaattg ctcataaagt gacgtaaagg
      541 cggataattt gcgcaactgc gtataacatt ttttacctta cataaaactg atcaacgtaa
      601 tttgccaccg ggaattcttt gtcaacaatt aactctgagc gcgcggcaac gctattcgac
      661 tggtatcaga cggatgaaat ccctataatt gccgcgtttg gcgcttcgtc gcccccttcc
      721 taacatctag gttaatcagg tcgctaaata tatgactgat cagtctcatc agtgcgtcat
      781 tatcggtatc gctggcgcat cggcttccgg caagagtctt attgccagta ccctttatcg
      841 tgaattacgt gagcaagtcg gtgatgaaca catcggcgta attcccgaag actgctatta
      901 caaagatcaa agccatctgt cgatggaaga acgcgttaag accaactacg accatcccag
      961 cgcgatggat cacagcctgt tgcttgagca tttacaagcg ttgaaacgcg gctcggcaat
     1021 tgacctgccg gtttacagct atgttgaaca tacgcgtatg aaagaaacgg tgacggttga
     1081 gccgaagaag gtcatcattc tcgaaggcat tttgttgctg acggatgcgc gtttgcgtga
     1141 cgaacttaac ttctccattt tcgttgatac cccgctggat atctgcctga tgcgccgcat
     1201 caagcgtgac gttaacgagc gtgggcgttc aatggattcc gtgatggcgc aatatcaaaa
     1261 aaccgtgcgc ccgatgttcc tgcaattcat tgagccttct aaacaatatg cggacattat
     1321 cgtgccgcgc ggcgggaaaa accgcatcgc gatcgatata ttgaaagcga aaataagtca
     1381 gttctttgaa taagcttgat aaattgtgta ccgttcagtg ataagctggt atgcccttga
     1441 cgtaattggc gttaagggag tgatgcgcga aaggagaaaa tgccatgcgt ctgtgtgacc
     1501 gagatattga agcctggctt gatgaaggcc gtttgtcgat caacccacgt ccgccagtgg
     1561 agcgtattaa cggcgcgacg gtggatgtac gcctgggcaa taaatttcgt accttccgtg
     1621 gtcacacggc agcgtttatc gatctgagcg gtcccaaaga tgaagtgagc gccgcgcttg
     1681 accgcgtgat gagcgatgag atcgttctcg acgagggcga ggcgttctat cttcacccag
     1741 gagagctggc gctggcggtg acgctggagt cggtgacgct gccagccgat ctggtgggct
     1801 ggctggacgg gcgttcctct ttggcgcgtc tggggctgat ggtgcacgtc accgcgcacc
     1861 gtatcgatcc gggctggtct ggttgcattg tgctggagtt ctacaactcc ggtaagctgc
     1921 cgctggcgct gcgtccgggc atgttaattg gtgcgctgag ctttgagccg ctttccggcc
     1981 cggcggcgcg accttacaac cgccgtgaag atgcgaaata tcgcaaccag cagggcgcgg
     2041 tagccagccg aatcgataaa gactaattca ggcccattga ggataccatg agacgatttc
     2101 tgacgacgct gatgatactc ttggtcgtgc tggtggccgg gttatctgcg ttagtgttgc
     2161 tggtgaatcc gaatgatttc cgcgactata tggtcaagca agttgctgca cgtagcggtt
     2221 atcaattgca gctcgacggg ccactgcgtt ggcacgtctg gccgcagctt agtatcctct
     2281 ccgggcgaat gtctctcacc gcccagggcg caagccagcc actggttcgc gccgacaaca
     2341 tgcgtctgga cgtggcgctt ttaccactac tgagtcatca actgagcgtt aagcaggtga
     2401 tgctaaaagg ggcagtgatc caactgacgc cgcagacgga agcggtgcgc agtgaagacg
     2461 ctccggttgc accgcgcgac aataccttgc cggatctgtc agacgatcgc ggatggtcgt
     2521 ttgatatatc cagtcttaag gtggcggaca gcgttctggt gttccagcat gaagatgacg
     2581 agcaggtgac aatccgcaat atccgcctgc aaatggaaca agatccccaa catcgtggct
     2641 catttgagtt ctccgggcgg gttaatcgcg atcagcgcga tctcacgata tcccttaacg
     2701 gtacggtaga tgcttctgat tatccgcatg atttaacagc ggctattgaa caaattaact
     2761 ggcagttgca gggtgccgat ttaccaaaac aaggtattca ggggcagggg agtttccagt
     2821 cccagtggca ggagtcacat aaacgccttt catttaacca aattagtttg accgccaatg
     2881 acagtacgct gagcgggcaa gcacaggtca cgctgacaga gaaaccggaa tggcagctga
     2941 gactgcaatt cccgcaactg aatcttgaca acctcatccc gcttaatgaa accgcgaatg
     3001 gtgaaaacgg tgccgcgcag caggggcaga gccaatcaac gttgccgcgc ccggtcattt
     3061 cttcgcgtat tgatgaacct gcctatcagg gactgcaagg ctttacagct gatattttgt
     3121 tgcaggccag taacgtgcgc tggcgcggaa tgaattttac agatgttgcc acgcaaatga
     3181 ccaacaagtc gggtttgctg gaaattactc aactgcaggg caagcttaac ggtggaaagg
     3241 tttcactgcc gggcacgctg gacgcgacat caataaatcc gcggataaac ttccagccac
     3301 ggctggagaa cgttgagatt ggtaccattc tgaaggcgtt taactatccg atttcgttga
     3361 ccggaaaaat gtcactggct ggtgatttct ccggtgctga catagatgcc gacgcattcc
     3421 gccacaactg gcaagggcag gcgcatgttg agatgaccga cacacgtatg gaagggatga
     3481 acttccagca gatgattcag caagcggtag agcgtaatgg cggtgatgtg aaggccgctg
     3541 aaaacttcga taacgtgacg cggcttgacc gctttaccac cgatttgacg ttgaaggatg
     3601 gcgtcgtgac gttaaacgac atgcaaggtc aatcgcctgt gctggcgctg acaggggaag
     3661 gcatgttgaa tctggcagat caaacctgcg acacccagtt tgatattcgg gtcgtgggtg
     3721 gctggaacgg ggaaagcaaa ctgattgatt tcctcaaaga aacgccagta ccgctgcggg
     3781 tttatggcaa ctggcagcaa ctcaattaca gcctgcaagt ggatcagtta ctgcgcaaac
     3841 atctacagga cgaagcgaaa cgtcgcctga atgactgggc cgagcggaat aaagattccc
     3901 gcaatggcaa agatgtgaag aagttgctgg agaagatgta ggttaccagg tgacctgtcc
     3961 tgtaggcctg ataagacgcg taaagcgtcg catcaggcgt tcgcggcggc actattatat
     4021 tctgaatatg taagatcgaa ggctgttccg ttcacttgat gttcctcgct actatggaac
     4081 gctcaagccg gtgaacgtgc cagggtggct caatcgccac caccctggca acccgggctc
     4141 ccggcggtaa atcgccgctg gcgcggttcc ctcatcttat tcctttcggc tatcgggtac
     4201 gggcgcgagg taacatccct gtaaaacgcg ccctcagccc acatccatgt gggctgcccc
     4261 agcctacagg aaacgattcg gcgatttaca gccggaccca caccaccgct gtgatgtccg
     4321 tttaccacag acaacgcttc attgctgaag ttataaggcg ttacgcagca ttgggtaaaa
     4381 tggccgatac catcacacct cgtactccat ctcgccatcc ttacgcagcg gtataatctg
     4441 caccttctgc acgcgatggc tttccacctg caacgtttta agcaaataat cccccacctg
     4501 aacttcttcg ccaggttttg gaatacgctg caaatactcc atcaacagcc cggcaatggt
     4561 gtgatattca cgtttttcat ccagcggcag cggcacatat tgcaccagat cctccagcgg
     4621 catatgacca ttcgccgtcc aggaaccgtc cgcattcttc tgaatatcat ggcgggcgtc
     4681 gatctcttcc acttcgttcg gtaagttacc ggcaatggtt tcagtgacgt cacttaatgt
     4741 cacaatccct tccaccgagc caaactcatc caccacaaaa gcaaagtgcg tgcgggcatt
     4801 acggaactgc tccagggctg gtaacaacgg caaggtttcc gggaacacca gcggctggcg
     4861 aatcaacacc cgcaggttga gcggttcgcc gcgcagtgac tgttgtagca ggtcgataac
     4921 gtgaacaaca ccgagcaaat cttctgcgtc atcaccgtcg gtaaccacca gccgcgtatg
     4981 ctgatttcgc tccagcaact ggcggatttc ttcttccggc gcgttgagat cgatatgctc
     5041 aatatcgtgg cgcgacgtca taatgctgct gacggtacgc tggttaagat taagtacccg
     5101 ctcaatcatc cgccgttcct gcggattaaa gatctgctgg tttccatgat ccaccagcat
     5161 ggacgcggtt tcggcgtcca gttccgcatc ttctttttgc ccgctaagca ggcgcattac
     5221 cgcttcggta gtccgctggc gtagcgtctg attagcggaa agaaaacgtc gccggttaaa
     5281 gatagccaac tgattgagtg cctcgatcat cactgagaaa ccaatggcag cgtacaggta
     5341 gcctttcgga atgacgaagc tgaaaccttc tgccaccagg ctaaagccaa tcatcaacag
     5401 gaagcttaag cagagaataa cgatggtcgg gtgactgtta acaaattgcg ttaacggctt
     5461 gctggccatc aacatcaggc tgatagcgat aaccacggcg gccatcatga ccagtaaatg
     5521 gtcgaccatc ccgacggcgg taatcactga gtcgagtgag aaaatggcgt ccagtaccac
     5581 aatttgcgtc accacgcccc agaacttcgc acctttgcgt tgtgtgggat tattgctgtc
     5641 tttcccttcc agccgttcgt tcagctccat cgtggctttg aacaacagga agaaaccacc
     5701 aaacagcatg attaagtcgc gggcgctaaa ggtaaacgag cggaagctga acagcggttg
     5761 agtcagggtg accagccagg agattgacgc cagcagtaac aggcgcatta acatcgccag
     5821 cagcagcccg gtaacccgtg cgcggtcgcg ctgcttcggc ggtagttttt cggcgaggat
     5881 ggcaataaag accaggttat caatgccgag gaccagttcg atcacaatca gcgtgattaa
     5941 cccggcccag atagacggat cggcaatcca ttccatagta agtacaaaac ctattcgttg
     6001 tatgacaaat gtcacaattc gatcatggat aaatcggggc aaaaatgcaa tgttgcagca
     6061 ctatttatct catattattt gagggtaata acctcacatt atccctgaat taaaagcggt
     6121 aataataaag cattatttaa gaaatatcgc atatggagac gcaatattaa atattgtcaa
     6181 cctaaagaaa ctcctaaaaa ccatattgaa tgacacttaa tataattctt aaaaatagcc
     6241 aattgccgaa ttgttacctt gcctgctatt ccgttagctg taacacttcc tcctgcatta
     6301 ttggagagcc aatattcaat taccataatg tcaccttata ttcggaagtt aatttccgca
     6361 ataacccttt ctcatcaacg attgtatgca gagagggttt gaaaaattaa gctcaatcag
     6421 tgtattggta gctaaaaagc caggggcggt agcgtgtctg gatgcctgaa agaccagtct
     6481 gaattattct gtcaatagcc tgcggatgaa atcaattttt ttaggactga tgccagttaa
     6541 atttttattc aattaactgc aaggtaaatg tgcatggcga cattgctatt aatagtgcac
     6601 aggataatta ctctgccaaa gtgataaata aacaatgatg aaatccaaaa tgaaattgat
     6661 gccattattg gtgtcagtaa ccttgataag cggttgcaca gtacttccgg gcagcaatat
     6721 gtcgacgatg ggcaaagacg tcatcaaaca gcaggacgct gatttcgatc tcgacaaaat
     6781 ggtgaatgtt tatccgctga ccccgcgcct gattgaccaa ttacgcccac gcccgaatgt
     6841 agcgcgcccc aatatgacgc tggaaagcga gatcgcgaat taccagtatc gcgtcgggcc
     6901 gggggacgtt cttaatgtca ccgtctggga tcacccggaa ctcaccacgc cagctggtca
     6961 gtaccgcagc tccagcgaca ccggcaactg ggtacagcct gacggcacta tgttttaccc
     7021 gtatatcggc aaggttcacg tagtcgggaa aacgctcgct gaaatccgca gtgatattac
     7081 cgggcgctta gcgacgtaca tcgctgaccc gcaggtggac gttaatatcg ccgccttccg
     7141 ctcacaaaag gcctatatct caggtcaggt gaataaatcc ggtcaacagg cgatcaccaa
     7201 cgtaccactg accattctcg acgccatcaa cgctgcaggt ggcctgaccg acaccgctga
     7261 ctggcgcaac gtggtgctaa cacacaatgg tcgtgaagaa cgcatttctt tacaggcgct
     7321 gatgcaaaac ggcgacctca atcagaaccg actgctttat cccggcgata ttctctacgt
     7381 gccgcgtaat gatgatctga aagtatttgt gatgggtgaa gtgaagaaac agagcaccct
     7441 gaaaatggac tttagcggca tgaccctgac tgaagctctg ggcaatgccg aaggtatcga
     7501 catgaccacc tccaacgcca gcggcatctt tgtcattcgt ccgctgaaag gcgagggcgg
     7561 gcgtaacggc aagattgcca atatctacca gctggatatg tccgatgcga cgtcgctggt
     7621 gatggcgaca gaattccgcc tgcaacctta tgacgtggtg tatgtcacca ccgccccggt
     7681 ttcccgctgg aaccgtctga tcaatcagtt gctgccaact attagcggtg tccgttacat
     7741 gacggataca gccagcgaca ttcataactg gtaatcgtca tgtttaacaa catcttagtt
     7801 gtctgtgtcg gcaatatttg ccgttccccg atggcggaac gcttactgca acgttatcac
     7861 ccggaactga aagtggagtc cgctggactc ggcgcgctgg tcggtaaggg cgctgatcct
     7921 accgctatca gcgtcgccgc agaacatcaa ctgtctctgg aaggccactg tgcacgacaa
     7981 atcagccgcc gcctgtgtcg caactatgac ctgattttga ccatggataa gcgccatatc
     8041 gaacgcttat gcgagatggc accggagatg cgcggcaaag tgatgctgtt tggtcactgg
     8101 gataacgaat gtgaaatccc cgatccgtat cgcaaaagcc gggaaacgtt tgcagcggtg
     8161 tacacattac ttgaacggtc tgcccgccag tgggcgcagg cattgaacgc agagcaggta
     8221 taagaatgac agaaaaagta aaacaacatg ccgctccggt aacgggcagt gatgaaatcg
     8281 atattggtcg cctggtcggc accgtcattg aagcgcgctg gtgggtgatt ggcatcaccg
     8341 ccgtatttgc cctctgtgcc gtggtttaca ctttcttcgc cacgccgatt tatagtgccg
     8401 acgcactggt acaaatcgag caaaacagcg gcaattcgct ggtgcaggat atcggctcgg
     8461 cattagccaa caaaccgcct gcatcggacg ccgagatcca gttgattcgt tcgcgtctgg
     8521 tgcttggtaa aacggtggac gatctcgacc tcgatattgc ggtgagcaaa aacacgttcc
     8581 cgattttcgg ggcgggctgg gatcgcctga tggggcgtca gaacgagacg gtgaaagtga
     8641 ctacctttaa ccgcccgaaa gagatggcgg atcaggtgtt tacgcttaat gtgctggatg
     8701 acaaaaacta caccctgagc agtgatggcg gctttagcgc ccgtgggcaa gcgggccaga
     8761 tgctgaaaaa agaaggcgtc acgctgatgg ttgaagccat tcacacccgc ccgggcagtg
     8821 agtttaccgt caccaaatac tccacgttgg ggatgatcaa ccaactgcaa aacagcctga
     8881 cggtaacgga gaacggcaaa gacgcaggcg tactgagcct gacttatacc ggtgaagatc
     8941 gcgaacagat ccgcgacatt cttaacagca tcgctcgtaa ctatcaggaa caaaatattg
     9001 agcgcaaatc ggcggaagcg tcgaaaagcc tcgctttcct cgcgcaacag ttaccggaag
     9061 tacgtagccg cctcgatgtt gccgaaaaca aactgaatgc cttccgtcag gataaagatt
     9121 ctgttgatct gccgcttgaa gcgaaagcgg tgctcgattc gatggtgaac atcgacgcgc
     9181 agttgaacga actgaccttt aaagaggcgg aaatctccaa gctgtacacc aaagttcacc
     9241 cggcgtaccg cacgctgctg gagaaacgtc aggcgctgga agaagagaaa gccaaactta
     9301 acggtcgcgt aacggcgatg ccgaaaaccc agcaggaaat tgtccgtctg acccgcgatg
     9361 tcgagtctgg tcagcaggtc tatatgcaac tgctgaataa agagcaggag ctgaaaatca
     9421 ccgaagccag caccgtcggc gatgtgcgca ttattgaccc ggcaatcacc cagcctggcg
     9481 tgctaaaacc gaagaaaggg ctgattatcc tcggcgcgat tatccttggc ctgatgctct
     9541 ctatcgtggg tgtgctgctg cgctcgttgt ttaatcgcgg catcgaaagc ccgcaagtgc
     9601 tggaagaaca cggaatcagc gtctatgcca gcatcccgct gtcggagtgg cagaaagcgc
     9661 gcgatagcgt caaaaccatc aaaggggtta aacgctataa acagagccag ctactggcag
     9721 tggggaatcc aaccgatctg gcgattgaag ccatccgtag tctgcgtacc agtctgcact
     9781 tcgcgatgat gcaggcgcag aacaatgtgt tgatgatgac cgggggtagc ccgtcaatcg
     9841 gtaaaacctt tgtctgcgcc aacctggcgg cggtgatcag ccagaccaat aaacgcgtgt
     9901 tgttgatcga ctgcgatatg cgcaaaggct acacccacga gctgttgggc accaataatg
     9961 tcaatgggct gtcggagatt ctgattggtc agggcgatat tactaccgct gctaaaccga
    10021 cctctattgc caaatttgac ctgatcccgc gcggtcaggt gccgccaaat ccttctgaac
    10081 tgttaatgag cgaacgcttt gccgaactgg tgaactgggc gagtaaaaac tatgacctgg
    10141 tgttgattga tacgccgccg attctggcag tgaccgatgc ggcaattgtt ggtcgtcatg
    10201 tcggaaccac gttaatggtg gcgcgttatg cggtcaacac attgaaagaa gtggaaacca
    10261 gtctgagccg ctttgagcaa aacggtattc cggtgaaagg ggtgattctg aactccatct
    10321 tccgccgcgc cagcgcgtat caggattatg gctattacga atacgaatat aagtcggatg
    10381 cgaaataacg acgcctgcat tcgcaccgcc ccgtaggccg gataaggcgc tccgccgcat
    10441 ccggcaagca aaccagctca taagccggga gaacaaccta tgaaagacaa tccgctgatc
    10501 tcaatctata tgccaacttg gaaccgccaa caactggcga ttcgggcgat aaaatcggtc
    10561 ctgcgccagg actacagcaa ctgggagatg atcatcgtgg atgattgctc cacctcctgg
    10621 gaacaactgc aacagtacgt cactgccctc aacgatccgc gtatcactta catccacaac
    10681 gacattaaca gcggggcgtg cgcggtgcgt aaccaggcga ttatgctggc gcaaggggaa
    10741 tacatcaccg ggatcgatga tgatgatgaa tggacgccca accgcctgag cgtcttcctc
    10801 gcccataaac agcaactggt cacacacgcc tttttgtacg ctaacgacta cgtttgccag
    10861 ggagaagttt attcccaacc ggcgagctta ccactgtatc ccaaatcgcc gtactcacgc
    10921 cgcctgttct ataaacgcaa tatcattggc aatcaggtct ttacctgggc atggcgtttc
    10981 aaagagtgtt tgttcgatac cgaactgaaa gcagcacagg attacgacat attcctgcgg
    11041 atggtggtgg agtacggcga accgtggaaa gtggaagagg cgacgcagat cctgcatatc
    11101 aatcacggcg aaatgcagat cacctcgtca ccgaaaaaat tctccggata cttccacttt
    11161 taccgcaagc acaaagacaa attcgaccgc gccagtaaaa aatatcagct gtttaccctc
    11221 taccagatcc gcaataaacg tatgacctgg cgcaccttgt tgacgctgct gtcggtgcgt
    11281 aacggcaaac gtctggctga tggtattcgg gggcgctaaa gatgctggaa gatctgcgcg
    11341 ccaacagctg gagtttacgc ccgtgctgca tggtacttgc ctatcgcgtc gctcattttt
    11401 gttcggtgtg gcgcaaaaag aacgtcctca acaatctgtg ggcggccccg ctgctggtgc
    11461 tgtatcgcat tatcaccgaa tgctttttcg gttatgaaat ccaggccgcc gcgaccattg
    11521 gccgccgctt tactatccat cacggttacg ccgtagtgat caataaaaac gtggtggcgg
    11581 gggatgattt caccattcgc cacggcgtca ctatcggcaa tcggggtgcc gataacatgg
    11641 catgtccaca cattggcaac ggcgtcgaac tcggtgccaa cgtcattatt cttggtgata
    11701 tcacgcttgg taacaacgtc accgtgggtg cgggcagcgt ggtgctcgac tctgtcccgg
    11761 acaacgcgct ggtggtggga gaaaaagcgc gagtgaaggt aattaaatga atattctgca
    11821 atttaatgtg cgactggcgg aaggcggggc agcaggtgtg gcgttagatc tccaccagcg
    11881 tgcgctgcaa caggggctgg cgtcacattt tgtctacggc tacggcaaag gcggcaaaga
    11941 gagcgtcagc catcagaact atccgcaggt catcaaacat acgccgcgga tgaccgcgat
    12001 ggcgaacatt gcgttgtttc gtctgtttaa tcgcgatctg ttcggcaatt tcaatgagtt
    12061 atatcgcacc attactcgca caccgggtcc ggtggtcctg cattttcatg tgctgcacag
    12121 ctactggttg aatcttaaca gcgtggtgcg cttttgcgaa aaagtgaaaa accataaacc
    12181 ggatgtcacc ctggtctgga cgctgcacga ccactggagt gtaaccggac gctgcgcctt
    12241 taccgacggt tgcgaaggct ggaaaacggg ctgccagaaa tgcccgacct taaataacta
    12301 tccgccggtg aagattgatc gcgcgcacca gctggtggcg ggcaaacgcc agttattccg
    12361 cgagatgctg gcgctgggct gtcagtttat ttcccccagc cagcatgtgg ctgacgcttt
    12421 caatagtctg tacgggccag ggcgttgccg gattatcaat aatggcattg atatggcaac
    12481 cgaagcgatt ctggcagatc tgcctccggt gcgcgaaact cagggcaagc cgaaaatcgc
    12541 ggtggtggcg catgacctgc gttacgacgg caaaactaac cagcaactgg tgcgtgagat
    12601 gatggcgctg ggcgacaaaa tcgaactgca tacctttggt aagttctcgc cgttcaccgc
    12661 tggcaacgtg gttaatcacg gctttgaaac tgacaagcgc aagttgatga gcgcgctcaa
    12721 tcagatggat gcgctggtgt tcagttctcg tgtcgataac tacccgctga ttttgtgtga
    12781 ggcgctatcg attggcgtgc cggtgattgc cacccatagc gatgcggcgc gggaagtgct
    12841 gcaaaaatcc ggtggtaaaa ccgtcagtga agaagaggtg ctgcaactgg tacagttaag
    12901 caaaccggaa atcgcgcagg cgatatttgg taccacgctg gctgagttca gccaacgcag
    12961 tcgcgccgcc tacagtggac aacagatgct ggaggagtat gtcaacttct atcagaatct
    13021 gtagctacct gctgctgcca ctgatctacc tgctggttaa cgtcaaaatc gcccagcttg
    13081 gcgaaagttt ccccatcacc atcgtcactt ttttacctgt cttgttactg ctgtttttag
    13141 aacgcatcag cattaaaaaa ttgatgattg ccttagggat tggcgcggga ctcacggcgt
    13201 ttaactacct gtttggtcag tcgttggatg ccggtaaata cgtcacttca actatgctgt
    13261 ttgtctatat tgtgatcatt attggcatgg tgtggagtat tcgttttaaa acaatttcgc
    13321 cacacaacca tcgtaagata ttacgtttct tttatctggt ggtcggactg gtggtggcgc
    13381 tggcggcggt ggagatggcg caaattatcc tcaccggtgg cagcagtatt atggagccga
    13441 tttcgaaata tctgatttac agcaacagct atgtgctgaa tttcattaaa ttcggcggca
    13501 agcgcacgac agcactttat ttcgaaccgg catttttcgc tctggcatta atctcaattt
    13561 ggctcagcat caaacagttt ggtatcaaaa cgcctaaaac agatgctatg attctcgcag
    13621 ggataatatt atccggatcg ttttcagggg ttatgacctt tatcctgttt tatttgctgg
    13681 agtgggcatt tcaatatctg aataaagagg tgattaagaa aaaattaccg ttagcattga
    13741 tttctctggc tgtattcctg gttggtgtgg taatcgcgtt tccttatatt tccacccgtc
    13801 tgggcgattt aggtacggaa ggatcgtcat cgtattatcg tattgtcggt ccgctggtga
    13861 tggtcggtta ttctttgacc catattgacg gtgtagtcag atttggctca ctttatgaat
    13921 atgttgcatc attcggaata tttaacggtg cggatgtcgg aaaaaccata gacaatggtt
    13981 tgtatctgct gattatttat ttttcctggt tcgcggtgtt tttatcgctg tggtacatgg
    14041 ggaaagtgat aaaaatgatg atcaacgctt ttagtgataa ccgcaatttt cgcgtgcaat
    14101 tatatctttt tactccggta tcgctgtttt ttaccggttc gatatttagc tcggaatatg
    14161 catttttaat cgtctgtccg tttattttgc gaaaagcgtt aaatattacg aggtaagaat
    14221 aagaacatgt tgcttagcat aatcactgtc gcgtttcgta acctcgaagg gatagtcaaa
    14281 acacatgcct cgctggtgca tctggcgcag gcggacgata tcagcttcga atggattgtt
    14341 gtcgatggcg gttccaacga cgctacccgt gagtatctgg aaaatctcaa tggtatctat
    14401 aacctacgct ttgtcagcga gccagataac ggtatctacg acgccatgaa caagggcatt
    14461 gcgatggcac aaggcaagtt cgcgttgttt ctcaactcgg gcgatatttt tcatcaggat
    14521 gccgcaaatt ttgtccgtaa gttaaaaatg caaaaagata acgtgatgat cactggcgat
    14581 gcgctgctgg attttggcga cgggcataaa attaaacgta gcgccaaacc gggctggtat
    14641 atttatcaca gcctgcccgc cagccatcag gcgatatttt tcccggtatc cggcttgaaa
    14701 aaatggcgtt atgacctgga atataaagtt tcttctgatt acgcgctggc agctaaaatg
    14761 tataaagccg gttatgcatt taaaaaactc aatggcctgg tgtctgaatt ttctatgggt
    14821 ggggtatcaa ccaccaataa tatggaattg tgtgctgacg ccaaaaaagt ccaacggcag
    14881 atattacatg tacctggctt ctgggcagaa ttatcctggc atttacgcca acgtacgaca
    14941 ggtaagacta aagccttata taacaaagtc tgaatataag gaaaaccaat gcaagattta
    15001 agcggtttct cggtgccgaa agggttccgg ggcggcaacg ctattaaagt gcaattatgg
    15061 tgggcagtac aggcaacaat atttgcctgg tcgccacaag tattgtatcg ctggcgggct
    15121 tttttattac gtttattcgg agcaaaaata ggaaaaaacg tagttattcg tccgtcagta
    15181 aaaattacct atccgtggaa attaacctta ggtgattacg cgtgggtcgg cgatgacgtc
    15241 aatttatata ccctcggtga aataaccatt ggcgcacatt cggtgatatc gcaaaaaagt
    15301 tatttatgca ccggtagcca cgaccatgca agtcaacatt tcaccattaa cgccacgcct
    15361 attgtgattg gcgagaaatg ctggctggca accgatgtct ttgttgccct aggcgtcaca
    15421 atcggcgacg gcaccgtcgt gggtgcacga agcagtgttt ttaaatcgct tccggcaaat
    15481 gtggtttgcc gggggaatcc cgcagtggtg atacgcgaac gcgttgaaac tgaataaatt
    15541 caaaaataca gaggaataag acatgtcaaa agtcgctctc atcaccggtg taaccggaca
    15601 agacggttct tacctggcag agtttctgct ggaaaaaggt tacgaggtgc atggtattaa
    15661 gcgtcgcgca tcgtcattca acaccgagcg cgtggatcac atttatcagg atccgcacac
    15721 ctgcaacccg aaattccatc tgcattatgg cgacctgagt gatacctcta acctgacgcg
    15781 cattttgcgt gaagtacagc cggatgaagt gtacaacctg ggcgcaatga gccacgttgc
    15841 ggtctctttt gagtcaccag aatataccgc tgacgtcgac gcgatgggta cgctgcgcct
    15901 gctggaggcg atccgcttcc tcggtctgga aaagaaaact cgtttctatc aggcttccac
    15961 ctctgaactg tatggtctgg tgcaggaaat tccgcagaaa gaaaccacgc cgttctaccc
    16021 gcgatctccg tatgcggtcg ccaaactgta cgcctactgg atcaccgtta actaccgcga
    16081 atcctacggc atgtacgctt gtaacggaat tctcttcaac catgaatccc cgcgccgcgg
    16141 cgaaaccttc gttacccgca aaatcacccg cgcaatcgcc aacatcgccc aggggctgga
    16201 gtcgtgcctg tacctcggca atatggattc cctgcgtgac tggggccacg ccaaagacta
    16261 cgtaaaaatg cagtggatga tgctgcaaca ggaacagccg gaagatttcg ttatcgcgac
    16321 cggcgttcag tactccgtac gtcagttcgt ggaaatggcg gcagcacagc tgggcatcaa
    16381 actgcgcttt gaaggcacgg gtgttgaaga gaagggcatt gtggtttccg tcaccgggca
    16441 tgacgcgccg ggcgttaaac cgggtgatgt gattatcgcc gttgatccgc gttacttccg
    16501 tccggcagaa gttgaaacgc tgctcggcga cccgaccaaa gcgcacgaaa aactgggctg
    16561 gaaaccggaa atcaccctca gagagatggt gtctgaaatg gtggctaatg acctcgaagc
    16621 ggcgaaaaaa cactctctgc tgaaatctca cggctacgac gtggcgatcg cgctggagtc
    16681 ataagcatga gtaaacaacg catttttatc gctggccatt gtgggatggt cggttctgcc
    16741 atcaagcggc agctcgaaca gcgcggtgat gtggagctgg tattacgtac ccgcgatgaa
    16801 ctgaacctgc tggacagccg cgccgtgcat gatttctttg ccagcgaacg cattgaccag
    16861 gtctatctgg cggcggcgaa agtgggcggc attgttgcca acaacaccta tccggcggat
    16921 ttcatctacc agaacatgat gattgagagc aacatcattc acgccgcgca tcagaacgac
    16981 gtgaacaaac tgatgtttct cggatcgtcc tgcatctacc cgaaactggc aaaacagccg
    17041 atggcagaaa gcgagttatt gcagggcacg ctggagccga ccaacgagcc ttacgccatt
    17101 gccaaaatcg ccgggatcaa actgtgcgaa tcttacaacc gccagtatgg acgcgattac
    17161 cgctctgtca tgccgaccaa cctgtacggg ccgcatgaca acttccaccc gagtaactcc
    17221 catgtgatac cagcatcgct gcgtcgcttc cacgaggcga cggcacagaa tgcgccggac
    17281 gtggtggtat ggggcagcgg tacaccgatg cgtgaattcc tgcacgtcga tgatatggcg
    17341 gcggcgagca ttcatgtcat ggagctggcg catgaagtct ggctggagaa tacccaaccg
    17401 atgctgtcgc acattaacgt cggcacgggc gttgactgca ctatccgcga gctggcgcaa
    17461 accatcgcca aagtggtggg ttacaaaggt cgggtggttt ttgatgccag caaaccggat
    17521 ggtacgccgc gcaaactgct ggatgtgacg cgcctgcatc agcttggctg gtatcacgaa
    17581 atctcactgg aagcggggct tgccagcact taccagtggt tccttgagaa tcaagaccgc
    17641 tttcgggggg aatgatgttt ttacgtcagg aagactttgc cactgtagtg cgctccacac
    17701 cgcttgtctc tctcgacttt attgtcgaga acagtcgcgg cgagtttctg cttggcaaaa
    17761 gaaccaaccg cccggcgcag ggttactggt ttgtgccggg agggcgcgtg cagaaagacg
    17821 aaacgctgga agccgcattt gagcggctga cgatggcgga actggggctg cgtctgccga
    17881 taacagcagg ccagttttac ggtgtctggc agcattttta tgacgataac ttctccggca
    17941 cggatttcac cactcactat gtggtgctcg gttttcgctt cagagtagcg gaagaagagc
    18001 tgttactgcc ggatgagcag catgacgatt accgctggct gacgccggac gcgctgctcg
    18061 ccagtaacga tgttcacgct aacagccgcg cctattttct cgctgagaag cgtgccggag
    18121 tacccggatt atgaaaatac tggtctacgg cattaactac tcgccggagt taaccggcat
    18181 cggcaaatac accggcgaga tggtggaatg gctggcggca caaggtcatg aggtgcgggt
    18241 cattaccgca ccgccttact acccgcaatg gcaggtgggt gagaactatt ccgcctggcg
    18301 ctacaaacga gaagaggggg ccgccacggt gtggcgctgc ccgctgtacg tgccaaaaca
    18361 gccgagcacc ctgaaacgct tgttgcatct cggcagtttt gccgtcagca gtttctttcc
    18421 actgatggcg caacgtcgct ggaagccgga tcgcattatt ggcgtagtgc caacgctgtt
    18481 ttgcacgccg ggaatgcgcc tgctggcgaa gctctctggt gcgcgtaccg tgctgcatat
    18541 tcaggattac gaagtggacg ccatgctggg gctgggcctt gccggaaaag gcaaaggcgg
    18601 caaagtggca cagctggcaa cggcgttcga acgtagcgga ctgcataacg tcgataacgt
    18661 ttccacgatt tcgcgttcga tgatgaataa agccatcgaa aaaggcgtgg cggcggataa
    18721 cgtcatcttc ttccccaact ggtcggaaat tgcccgtttt cagcatgttg cagacgccga
    18781 tgttgatgcc cttcgtaacc agcttggcct gccggataac aaaaaaatca ttctttactc
    18841 cggcaatatt ggtgaaaagc aggggctgga aaacgttatt gaagctgccg atctcctgcg
    18901 cgatgaaccg ctgatttttg ccattgtcgg gcagggcggc ggcaaagcgc ggctggaaaa
    18961 aatggcgcag cagcgtggac tgcgcaacat gcaatttttc ccgctgcaat cgtatgacgc
    19021 tttacccgca ctgctgaaga tgggcgattg ccatctggtg gtgcaaaaac gcggcgcggc
    19081 agatgccgta ttgccgtcga aactgaccaa tattctggcg gtaggcggta acgcggtgat
    19141 taccgcagag gcccacacag aactggggca gctttgcgaa acctttccgg gtattgcggt
    19201 ttgcgtagaa ccggaatcgg tcgaggcgct ggtggcgggg attcgtcagg cgctcctgct
    19261 gcccaaacat aacacggtgg cacgtgaata tgccgaacgc acgctcgata aagagaacgt
    19321 gttacgtcaa tttataaatg atattcgggg ataattatgg cgcagtcgaa actctatcca
    19381 gttgtgatgg caggtggctc cggtagccgc ttatggccgc tttcccgcgt actttacccc
    19441 aagcagtttt tatgcctgaa aggcgatctc accatgctgc aaaccaccat ctgccgcctg
    19501 aacggcgtgg agtgcgaaag cccggtggtg atttgcaatg agcagcaccg ctttattgtc
    19561 gcggaacagc tgcgtcaact gaacaaactc accgagaaca ttattctcga accggcaggg
    19621 cgtaacactg cacctgccat tgcactggcg gcgctggcgg caaaacgtca tagcccggag
    19681 agcgacccgt taatgctggt attggcggcg gatcatgtga ttgccgatga agacgcgttc
    19741 cgtgccgccg tgcgtaatgc catgccttat gccgaagcgg gcaagctggt gacctttggc
    19801 attgtgccgg atctaccaga aaccggttat ggctatattc gtcgcggtga agtgtctgcg
    19861 ggtgagcagg atacggtggc ctttgaagtg gcgcagtttg tcgaaaaacc gaatctggaa
    19921 accgcccagg cctatgtagc aagcggcgaa tattactgga acagcggcat gttcctgttc
    19981 cgcgccggac gctatctcga agaactgaaa aaatatcgcc cggatatcct cgatgcctgc
    20041 gaaaaagcga tgagcgccgt cgatccggat ctcgatttta ttcgtgtgga tgaagaagcg
    20101 tttctcgcct gcccggaaga gtcggtggat tacgcggtca tggaacgtac ggcagatgct
    20161 gttgtggtgc cgatggatgc gggctggagc gatgttggtt cctggtcttc attatgggag
    20221 atcagcgccc acaccgccga gggcaacgtt tgccacggcg atgtgattaa tcacaaaact
    20281 gaaaacagct atgtgtatgc cgaatcaggc ctggtcacca ccgtcggggt gaaagatctg
    20341 gtagtggtgc agaccaaaga tgcggtgctg attgccgacc gtaacgcggt acaggatgtg
    20401 aaaaaagtgg tcgagcagat caaagccgat ggtcgccatg agcatcgggt gcatcgcgaa
    20461 gtgtatcgtc cgtggggcaa atatgactct atcgacgcgg gcgaccgcta ccaggtgaaa
    20521 cgcatcaccg tgaaaccggg cgagggtttg tcggtacaga tgcaccatca ccgcgcggaa
    20581 cactgggtag tggtcgcggg aacggcaaaa gtcaccattg atggtgatat caaactgctt
    20641 ggtgaaaacg agtccattta tattccgctg ggggcgacgc actgcctgga aaacccgggg
    20701 aaaattccgc tcgatttaat tgaagtgcgc tccggctctt atctcgaaga ggatgatgtg
    20761 gtgcgcttcg cggatcgcta cggacgggtg taaacgtcgc atcaggcgct gtttgtcgga
    20821 tgcggcgtga acgccttatc cgacctacgg ttcggttttg taggcctgat aagacgcggc
    20881 agcgtcgcat caggcaatga atgcgaaacc gcggtgtaaa taacgacaaa aataaaattg
    20941 gccgtttcgg tcagggccaa ctattgcctg aaaaagggta acgacatgaa aaaattaacc
    21001 tgctttaaag cctatgatat tcgcgggaaa ttaggcgaag aactgaatga agatatcgcc
    21061 tggcgcattg gtcgcgccta tggcgaattt ctcaaaccga aaaccattgt gttaggcggt
    21121 gatgtccgtc tcaccagcga aaccttaaaa ctggcgctgg caaaaggttt acaggatgcg
    21181 ggcgtcgatg tgctggatat cggtatgtcc ggcaccgaag agatctattt cgccacgttc
    21241 catctcggcg tggatggcgg cattgaagtt actgccagcc ataatccgat ggattacaac
    21301 ggcatgaagc tggtgcgcga gggggctcgc ccgatcagcg gtgataccgg actgcgcgac
    21361 gtccagcgtc tggctgaagc caacgacttt cctcccgtcg atgaaaccaa acgcggtcgc
    21421 tatcagcaaa tcaacctgcg tgacgcttac gttgatcacc tgttcggtta tatcaatgtc
    21481 aaaaatctca cgccgctcaa gctggtgatc aactccggga atggcgcagc gggtccggtg
    21541 gtggacgcca ttgaagcccg ctttaaagcc ctcggcgcgc ccgtggaatt aatcaaagta
    21601 cacaacacac cggacggcaa tttccccaac ggtattccta acccgctgct gccggaatgt
    21661 cgcgacgaca cccgcaatgc ggtcatcaaa cacggcgcgg atatgggcat tgcctttgat
    21721 ggcgattttg accgctgttt tctgtttgac gaaaaagggc agtttatcga gggctactac
    21781 attgtcggcc tgctggcaga agcgttcctc gaaaaaaatc ccggcgcgaa gatcatccat
    21841 gatccgcgtc tctcctggaa cactattgat gtggtgactg ctgcaggcgg cacgccggta
    21901 atgtcgaaaa ccggacacgc ctttattaaa gaacggatgc gcaaggaaga cgccatctac
    21961 ggtggcgaaa tgagcgccca ccattacttc cgtgatttcg cttactgcga cagcggcatg
    22021 atcccgtggc tgctggtcgc cgaactggtg tgcctgaaag gaaaaacgct gggcgaactg
    22081 gtgcgcgacc ggatggcggc gtttccggca agcggtgaga tcaacagcaa actggcgcaa
    22141 cccgttgagg cgattaaccg cgtggaacag cattttagcc gtgaggcgct ggcggtggat
    22201 cgcagcgatg gcatcagcat gacctttgcc gactggcgct ttaacctgcg cacctccaat
    22261 accgaaccgg tggtgcgcct gaatgtggaa tcgcgcggtg atgtgccgct gatggaagcg
    22321 cgaacgcgaa ctctgctgac gttgctgaac gagtaatgtc ggatcttccc ttaccccact
    22381 gcgggtaagg ggctaataac aggaacaacg atgataaatc taaaaaagcg tgagcgagcg
    22441 aaaaccaatg catcgttaat ctctatggtg caacgctttt cagatatcac catcatgttt
    22501 gccgggctat ggctggtttg cgaagtcagc ggactgtcat tcctctacat gcacctgttg
    22561 gtggcgctga ttacgctggt ggtgttccag atgctgggcg gcatcaccga tttttatcgc
    22621 tcatggcgcg gtgttcgggc agcgacagaa tttgccctgt tgctacaaaa ctggacctta
    22681 agcgtgattt tcagcgccgg actggtggcg ttcaacaatg atttcgacac gcaactgaaa
    22741 atctggctgg cgtggtatgg gctgaccagc atcggactgg tggtttgccg ttcatgtatt
    22801 cgcattgggg cgggctggct gcgtaatcat ggctacaaca agcggatggt cgccgtagcg
    22861 ggggatttag ccgccggaca aatgctgatg gagagtttcc gtaatcagcc gtggttaggg
    22921 tttgaagtgg tgggcgttta ccacgacccg aaaccgggcg gcgtttctaa cgactgggcg
    22981 ggtaacctgc aacagctggt cgaggatgca aaagcgggca agattcataa cgtctatatc
    23041 gcgatgcaaa tgtgcgacgg cgcacgagtg aaaaaactgg tccatcaact ggcggatacc
    23101 acctgttcgg tgctgctgat ccccgacgtc tttaccttca acattctcca ttcacgtctc
    23161 gaagagatga acggcgttcc ggtggtgccg ctgtatgaca cgccgctttc gggggttaac
    23221 cgcctgctaa aacgtgcgga agacattgtg ctggcgacgc ttattctgct gctgatctcc
    23281 ccggtgctgt gctgtattgc gctggcggtg aaactcagtt caccagggcc ggttattttc
    23341 cgccagactc gctatggtat ggatggcaag ccgatcaaag tgtggaagtt ccgttccatg
    23401 aaagtgatgg agaacgacaa agtggtgacc caggcgacgc agaacgatcc gcgcgtcact
    23461 aaagtgggga actttctgcg ccgcacctcg ctggacgaat tgccgcagtt tatcaatgtg
    23521 ctgaccgggg ggatgtcgat tgtcggtcca cgtccgcacg cagtagcgca taacgaacag
    23581 tatcgccagc tcattgaagg ctacatgctg cgccataaag tgaaaccggg cattaccggc
    23641 tgggcgcaga ttaatggctg gcgcggcgaa accgacacgc tggagaaaat ggaaaaacgc
    23701 gtcgagttcg accttgagta catccgcgaa tggagcgtct ggttcgatat caaaatcgtt
    23761 ttcctgacgg tattcaaggg cttcgttaac aaagcggcat attgatatga gcttacgtga
    23821 aaaaaccatc agcggcgcga agtggtcggc tattgccacg gtgatcatca tcggcctcgg
    23881 gctggtgcag atgaccgtgc tggcgcggat tatcgacaac caccagttcg gcctgctgac
    23941 tgtgtcgctg gtgattatcg cgctggcaga tacgctttct gacttcggta tcgctaactc
    24001 gattattcag cgaaaagaaa tcagtcacct tgaactcacc actctgtact ggctgaacgt
    24061 cgggctgggg cttgtggtat gtgtggcggt gtttttgttg agtgatgtca tcggcgacgt
    24121 gctgaataac ccggacctgg caccgttgat taaaacattg tcgctggcgt ttgtggtgat
    24181 cccccacggg caacagttcc gcgcgttgat gcagaaggag ctggagttca acaaaatcgg
    24241 catgatcgaa accagcgcgg tgctggcggg cttcactttt acggtagtta gcgcccattt
    24301 ctggccgctg gcgatgaccg cgatcctcgg ttatctggtc aatagtgcgg tgagaacact
    24361 gctgtttggc tactttggac gcaaaattta ttgccccggt ctgcacttct cgctggcgtc
    24421 tgtggcaccg aacttacgtt ttggtgcctg gctgacggcg gacagcatca tccactatct
    24481 caacaccaac ctttcaacgc tggtgctggc gcgtattctt ggcgcgggcg tggcaggggg
    24541 atacaacctg gcgtacaacg tggccgtcgt accgccgatg aagctgaacc caatcatcac
    24601 ccgcgtgttg tttccggcgt ttgccaaaat tcaggacgac accgaaaagc tgcgtgttaa
    24661 cttctacaag ctgctgtcga tagtggggat tatcaacttt ccggcgctgc tggggctgat
    24721 ggtggtgtcg aataacttcg taccgctggt attcggcgaa aagtggaaca gcattattcc
    24781 ggtgctgcaa ttgctgtgta ttgtgggtct gctgcgctcc gtgggtaacc cgattggttc
    24841 gctgctgatg gcgaaagcgc gggtcgatat cagctttaaa ttcaacgtat tcaaaacatt
    24901 tctgtttatt ccggcgattg ttattggtgg gcagatggcg ggcgcaatcg gcgtcacgct
    24961 cggtttcctg ctggtgcaga ttatcaacac cattctgagt tacttcgtga tgattaaacc
    25021 ggttcttggt tccagttatc gccagtacat cctgagtttg tggctgccgt tttatctctc
    25081 gttgccgacg ctggtggtca gttatgcgct gggcattgtg ctgaaagggc aactggcgct
    25141 ggggatgctg ctggcggtgc aaatagccgc gggcgtgctg gcgtttgtgg tgatgattgt
    25201 gctgtcacgc catccgctgg tggtggaagt gaagcgtcag ttttgtcgca gcgaaaaaat
    25261 gaaaatgctt ttacgggcgg ggtgaattgc tgttttcggt tccgacaacg gttgccggat
    25321 gcggcgtaaa acgccttatc cgtcctacgg ttctgtgctc gggacctgta ggcctgataa
    25381 gacgcgccag cgtcgcatca ggcaaaaaac ggcaacgaac acaacaaaca cagcctcacc
    25441 tggcatcaaa tttatgagga aaaaatgaaa ttacttattc tgggcaacca cacttgcggc
    25501 aatcgtggcg acagcgccat cttgcgcggc ttacttgatg ccatcaacat cctcaatcca
    25561 cataccgaag tggacgtgat gagccgctat ccggtcagtt cttcctggct gctcaaccgc
    25621 ccggtaatgg gcgatccgct gttcctgcag atgacacaac acaatagcgc ggcgggcgtt
    25681 gtcgggcgcg ttaaaaaagt cctccgtcgc cgctaccagc atcaggtatt gctctcacgc
    25741 gtcaccgaca ctggcaagct gcgcaatatc gccatcgccc agggattcac cgacttcgtg
    25801 cgcctgctgt caggttacga cgccattatt caggtcggcg gatcgttttt tgtcgatctc
    25861 tacggcgtgc cgcagtttga acatgcgctt tgcacattta tggcgaaaaa gccgctgttt
    25921 atgattggtc acagcgtcgg ccccttccag gatgagcaat ttaaccaact ggcgaactat
    25981 gtctttggtc actgcgacgc gctgatcctg cgcgaatcgg tcagccttga tttgatgaaa
    26041 cgcagcaata tcaccaccgc aaaagtggaa catggcgtcg ataccgcgtg gctggtcgat
    26101 caccacaccg aagacttcac cgccagctat gccgttcaac actggctgga cgttgccgca
    26161 caacagaaaa cagtggcaat taccctgcgc gaactggcac cgttcgacaa acgtctcggc
    26221 accactcaac aagcgtatga aaaagccttt gccggggtgg tcaatcgcat tctcgatgaa
    26281 ggctatcagg tgattgcgct ctccacctgt acgggtatcg acagctataa caaagacgac
    26341 cgcatggtgg cgctcaatct gcgccagcac atcagcgatc ctgcccgtta ccacgtagtg
    26401 atggatgaac tcaacgatct ggaaatgggc aaaattctcg gagcctgtga actcatcgtc
    26461 ggtacgcgcc tgcactccgc cattatctca atgaactttg ctactccggc gattgccatc
    26521 aactatgaac ataaatccgc cgggattatg cagcagctgg gactgccaga gatggcgatt
    26581 gatatccgtc atttactgga cggcagcctg caagcgatgg ttgcggatac cttaggccag
    26641 cttccggtgc tgaacgctcg acttaacgaa gccgtcagtc gcgaacgtca gacgggaatg
    26701 cagatggtgc agtctgtgct tgagcgcatc ggggaggtga aatgaaggtc ggcttcttct
    26761 tattgaaatt cccgctgtcg tcggaaacct tcgtcctcaa tcaaattacc gcgtttattg
    26821 atatgggctt tgaagtggag attgtcgctt tgcaaaaagg cgatacagag aacacccacg
    26881 cggcatggac gaaatacaac cttgtcgcca gaacccgctg gttacaggac gaacctgcgg
    26941 gcaaagtagc gaaactgcgc caccgcgcca gccagacctt acgtggcatt catcgtaaaa
    27001 atacctggca tgcgctcaac ctcaaacgct atggtgccga gtcgcggaac ctgattttgt
    27061 ctgccatttg tggtcaggtc gcaacaccgt ttcgcgccga tgtgttcatc gctcattttg
    27121 gcccggcggg ggtaaccgca gcaaaactac gcgaactggg tatcattcgc ggcaaaattg
    27181 ccaccatctt ccacggtatt gatatctcca gtcgggaagt gctcaaccac tacactcccg
    27241 aatatcaaca actgtttcgc cgtggcgacc tgatgttacc gataagcgat ctgtgggccg
    27301 gaaggctgca aaaaatgggc tgccccagag aaaaaatcgc cgtatcgcgt atgggcgtgg
    27361 acatgacgcg ctttagcccg cgtccggtga aagcgcccgc aacgccgctg gaaatcatat
    27421 ctgtcgcacg cttaaccgag aaaaaaggcc tgcatgtggc gattgaagcc tgccgtcagt
    27481 tgaaagagca gggcgtggca tttcgctatc gcatcctcgg cattggcctg tgggaacgac
    27541 gcctgcgcac cctcatcgaa caatatcaac tggaagatgt ggtagagatg ccgggcttta
    27601 aaccgagcca tgaagtgaag gagatgctcg acgacgcgga tgtcttcctg ttgccatcgg
    27661 taacgggcgc ggatggcgat atggaaggca ttccggtggc gctaatggaa gcgatggcgg
    27721 tcggtattcc ggtggtttct actctgcata gcggaattcc tgaactggtg gaggccgata
    27781 aatccggctg gctggtgcct gagaacgatg ctcgcgcact ggcacaacgt ctggcggcgt
    27841 ttagccaact ggataccgac gaactggctc cggtcgtcaa acgtgcgcgc gaaaaagtcg
    27901 aacacgattt taaccagcag gtgattaatc gagaactcgc cagcttgctg caggctttat
    27961 agaggagaac gtatgccatt taaaacactc tcccgacgca ccttcctgac ggcaagctcg
    28021 gcgcttgcct tcctccatac ccctttcgct cgcgcacttc ccgcccgaca aagcgttaac
    28081 attaacgact acaacccaca tgactggatc gcctcattta aacaagcctt cagcgaaggg
    28141 caaacggtcg tcgtgcctgc cggattcgtt tgtgacaata tcaacaccgg catcttcatc
    28201 cctcccggca aaacgttaca catccttgga agcctgcgcg gcaacggcag agggcgattt
    28261 gtcttacagg acggcagcca ggtgacaggg ggagagggcg gcggtatgca taacattacc
    28321 ctggatgtgc gtggttctga ctgcaccatc aaagggctgg cgatgagcgg ctttggcccg
    28381 gtaatgcaga tttatattgg cggcaaaaac aaacgggtca tgcgcaacct gaccatcgat
    28441 aacctcactg tcagccacgc taattacgcc atcttacgcc agggatttca taaccagatt
    28501 atcggtgcca acattaccaa ctgtaagttc agcgacttac agggcgacgc cattgaatgg
    28561 aacgtggcaa ttaacgacag tgatattttg atatctgacc atgtcatcga gcgtatcaac
    28621 tgtaccaacg gcaaaatcaa ctggggcatc ggcataggcc ttgcgggaag cacttatgat
    28681 aacaactacc cggaagacca ggcagtgaaa aactttgtcg tggcgaatat cacgggatcg
    28741 gattgtcggc agttgatcca tgttgaaaat ggtaaacatt ttgttattcg taatatcaat
    28801 gcccgcaata tcacgccgga tttcagtaag aaagcgggca ttgataacgc gacagtcgct
    28861 atttacggtt gtgacaattt cgtgattgat aatattgaaa tgattaatag cgccgggatg
    28921 ttaatcggct atggggtaat taaaggcaaa tatctctcga taccgcaaaa tttccgagtg
    28981 aataatattc aactggataa tacccatctt gcttataaat tgcgcggcat ccaaatctcc
    29041 gccgggaatg ccgtctcctt tgtgtcgcta actaacattg agatgaagcg tgcctcgctg
    29101 gagttacaca acaagccgca acatcttttt atgcgaaata tcaatgtgat gcaggaatcc
    29161 tcagttggac cagcattgag catgaacttc gacatgcgca aagacgttcg cggcgtcttt
    29221 atggcgaaaa aagaaacact gctgtctctt gcaaatgttc atgcggtgaa tgaaagaggg
    29281 caaagctccg tcgatatcga caggattaat caccatattg ttaatgtgga aaagattaac
    29341 tttagattgc cggaacggag ggagtagatt tgcgaccatt cctggaaaaa tggagccata
    29401 cttaggaaca atgctactgc aatccacaac gaagcggcgt aacatcacaa gtaattcagt
    29461 aatcaattca gggtaattga tgctggcgaa aaaaatcgaa caagctataa ttcagcaacc
    29521 attttacagg tggatgaaat aatgacgaat ttaaaagcag ttattcctgt agcgggtctc
    29581 gggatgcata tgttgcctgc cactaaggcg atacccaaag agatgctacc aatcgtcgac
    29641 aagccaatga ttcagtacat tgttgacgag attgtggctg cagggatcaa agaaatcctc
    29701 ctggtaactc acgcgtccaa gaacgcggtc gaaaaccact tcgacacctc ttatgagtta
    29761 gaatcactcc ttgagcagcg cgtgaagcgt caactgctgg cggaagtaca gtccatctgt
    29821 ccgccgggcg tgaccattat gaacgtgcgt cagggcgaac ctttaggttt aggccactcc
    29881 attttgtgtg cgcgacctgc cattggtgac aacccatttg tcgtggtact gccagacgtt
    29941 gtgatcgacg atgccagcgc cgacccgcta cgttacaacc ttgctgccat gattgcacgt
    30001 ttcaacgaaa cgggccgcag ccaggtgctg gcaaaacgta tgccgggtga cctctctgaa
    30061 tactccgtca tccagactaa agagccgctg gaccgtgagg gtaaagtcag ccgcattgtt
    30121 gaatttatcg aaaaaccgga tcagccgcag acgctggact cagacatcat ggccgtaggt
    30181 cgctatgtgc tttctgccga tatttggccg gaactggaac gtactcagcc tggtgcatgg
    30241 ggacgtattc agctgactga tgctattgcc gagctggcga aaaaacaatc cgttgatgca
    30301 atgctgatga ccggcgacag ttacgactgc ggcaaaaaaa tgggctatat gcaggcgttt
    30361 gtgaagtatg gcctacgcaa cctgaaagaa ggggcgaagt tccgtaaagg tattgagaag
    30421 ctgttaagcg aataatgaaa atctgaccgg atgtaacggt tgataagaaa attataacgg
    30481 cagtgaagat tcgtggcata agttttgctc attgaatttt cctgccgttg ccgtttttat
    30541 aaagcctata aaaacaagaa gttacatagc tcatattatt caaatttttc caggattttc
    30601 cttgtttcta cggctgtttg gtaagacaat taacgtttga atttttcgga tttggcgcga
    30661 gtggctaacg ctcgccacat cgttgagatg catgcagtgc actggtagct gttaagccag
    30721 gggcggtagc gtgtattaat acctctatta atcaaatctg gagcagtcta tttcacagca
    30781 tgctctatag ctatatggaa taaaaaagtg aagatacttg ttactggtgg cgcagggttt
    30841 attggttctg ctgttgttcg tcacataata aataatacgc aagatagtgt tgttaatgtc
    30901 gataaattaa catacgccgg gaacctggaa tcactcgctg atgtttctga ttctgaacgt
    30961 tatgcttttg agcatgcaga tatctgcgat gctgtagcca tgtcgcgtat tttcgcacag
    31021 caccagccag acgcggtgat gcacctggcg gcagagagcc acgttgaccg ttcaataacg
    31081 ggccctgcgg catttattga aaccaatatt gtggggactt atgttctttt agaagcggcg
    31141 cgcaattact ggtctgctct gaatgatgaa aagaaaaaaa gtttccgctt tcatcatatt
    31201 tctactgatg aagtttatgg tgacttaccc catccggatg aagcaaataa taacgaagcg
    31261 ttaccgctat ttacggaaac gacagcttac gcgccaagta gcccgtattc tgcgtctaaa
    31321 gcttccagcg atcatttggt acgtgcctgg aagcggactt atggtttacc aaccattgtg
    31381 actaattgct cgaacaatta tggtccttat catttcccgg aaaaacttat tccattggtt
    31441 attctgaatg ctctggaagg taaggcatta cctatttatg gtaaagggga tcaaattcgc
    31501 gactggttgt atgtagagga tcatgctcgt gcgttatata ccgtcgtaac cgaaggtaaa
    31561 gcgggtgaaa cttataacat tggtggacat aacgaaaaga aaaacatcga cgtagtgctc
    31621 actatttgtg atttgttgga tgagattgta ccgaaagaga aatcttatcg tgagcaaatt
    31681 acttatgttg ccgatcgccc ggggcatgac cgccgttacg ccattgatgc cgataaaatt
    31741 agccgcgaat tgggctggaa accgcaagaa acgtttgaga gcgggattcg taaaacggtg
    31801 gaatggtatc tggctaatac aaattgggtt gagaatgtga aaagcggtgc ttatcagtcg
    31861 tggattgaac aaaactatga gggccgtcag taatgaatat cctgcttttc ggcaaaacag
    31921 ggcaggtggg ttgggaactg cagcgtgctc tggctcctct gggtaacttg attgcgctcg
    31981 atgttcactc cactgattat tgtggagatt tcagcaaccc cgaaggtgtg gctgaaaccg
    32041 tcaaaaaaat tcgccccgat gttattgtta atgctgctgc tcacactgca gtagacaaag
    32101 cggaatcaga accgaatttc gcgcaattac ttaacgcgac atgcgtcgaa gcgattgcaa
    32161 aagcagctaa tgaagtcgga gcctgggtta tacactactc cactgattat gttttcccag
    32221 gcaacggtga cacgccatgg ctggaaacgg atgcaacagc accgctaaat gtctacggtg
    32281 aaaccaagct agctggggaa aaagcgttac aagaacattg cgcaaagcat cttattttcc
    32341 gtaccagctg ggtatacgct ggtaaaggaa ataactttgc caaaacgatg ttgcgcctgg
    32401 caaaagaacg cgaagagttg gctgtaataa acgatcaatt cggcgcacca acgggggctg
    32461 aattactggc tgattgcacc gctcatgcca ttcgcgtggc cgcaaataaa ccagaagttg
    32521 ctggtttgta ccatctggtt gctggtggta caacaacctg gcacgattat gccgctctgg
    32581 tatttgaaga agcgcgcaga gcggggatca accttgctct taacaaactt aatgccgtgc
    32641 cgacaacggc ctatcccaca ccagcacgtc gtccccataa ttctcgcctc aataccgaaa
    32701 aatttcagca gaactttgcg cttgtcttgc ctgactggca ggtgggcgtg aaacgtatgc
    32761 tcaacgaatt atttacgacc acggcaattt aacaaattat tgcatctcgc tcatgatgcc
    32821 agagcgagat gaattaaaag gaatggtgaa atgaaaacgc gtaaaggtat tattctggct
    32881 ggtggttccg gcactcgtct ttatcctgtg actatggctg tgagcaaaca actgctaccg
    32941 atttatgata aaccgatgat ttattatccg ctttcaacgc ttatgttggc gggtattcga
    33001 gatattctga ttatcagtac gccgcaggat acaccacgtt ttcaacaatt gcttggtgat
    33061 ggaagccagt gggggcttaa tctgcaatat aaagtacaac cgagtccgga tggtctggcg
    33121 caagcattta tcattggtga agaatttata ggtggtgatg attgtgcgct ggtacttggt
    33181 gataatatct tctatggtca cgacctgccg aaattaatgg ataccgctgt taacagagag
    33241 agtggcgcaa cggtttttgc ctatcacgta aatgatccag agcgctacgg tgtggtggaa
    33301 tttgatgata acggtacggc aattagtctg gaagaaaaac cgctggaacc aaaaagtaac
    33361 tatgcggtta ctgggcttta tttctatgac aatgacgttg tagaaatggc gaaaaacctt
    33421 aaaccatctg cacgtggcga actggaaatt accgatatta accgtattta tatggaccaa
    33481 ggacgtttgt ctgtcgctat gatggggcgt ggttatgcat ggctggacac ggggacacat
    33541 caaagtctta ttgaagcaag caacttcatt gcaacaattg aagagcgtca ggggctgaaa
    33601 gtttcctgcc cggaagaaat tgcttaccgt aaaggcttta ttgatgctgg gcaagtgaag
    33661 gtattagccg agccgctgaa gaaaaatgct tatggtcagt atctgctgaa aatgattaaa
    33721 ggttattaat aaaatgaacg taattaaaac tgaaattcct gatgtgctaa tttttgaacc
    33781 aaaagtattt ggtgatgagc ggggcttttt tatggaaagc ttcaatcaga aagttttcga
    33841 agaggctgta ggacgaaagg ttgaatttgt tcaggataac cattctaaat caactaaggg
    33901 tgtgttacgc ggactgcact atcagctgga accttatgct caaggaaaac tggtacgctg
    33961 tgttgtcggt gaagtttttg acgtagctgt tgatattcgg aaatcatcac caacatttgg
    34021 caagtgggtt ggtgtaaatt tgtcggcgga gaataagcgt caattatgga taccggaagg
    34081 attcgctcat gggttttgcg ttcttagtga tgaagcagaa tttgtctaca aaacaaataa
    34141 tttctattca aaaatgcaag agcgtgggat tttatggagc gacaaaagta taaatataga
    34201 atggcaggtt caaaatccat tgctttctga taaagatatt aatggtcaaa aatttgtaga
    34261 tgctgattat tttatatgat aaagaaatgt aataatgagc ataataaaaa atagtgtctg
    34321 gaaccttttt ggctatgcaa taccaacttt aattgctatc ccctcgctag gatttctcgc
    34381 tcgaggtttg gggcctgaag gtttcggtgt ttatacaatt gcaattgcac ttgttgggta
    34441 tgctggaatt tttgatgtag gcctgactcg ctctgttatt agagaaattg caattcatcg
    34501 tgataatcat catgaaagaa ccaaggtaat ttcaacaagt acatcttttt tggtgctatt
    34561 ttcatgcttt ggtgcttttt tattattgat tttctctgat ggaattgtta attatctaaa
    34621 aatttctggt gttgaacata gtgatataca actagcattt aaactgttgg ctatttgcat
    34681 tccattattt attctaaatc aattatggtc agccattctt gagggggatg aaaaatttgg
    34741 cattgtaaat attcaaaaat ctatatcaag ctcttgcatc gcgggaattc cggccatatt
    34801 tgttttttat agtgctacat tgtcggcagc ggttgctggt ttaatatttg caagagttat
    34861 ttcgatttta gtctctgcct attatgtcag gaatgatatt aaaatttcgg gggttcattt
    34921 ttgttataaa acctttaaac gactcttttt ctttggcggt tggatgacag taagtaatat
    34981 cataagtccg gtcatggtgt attttgatcg atttatagtg tcaaatatta tgggggcaga
    35041 taaagttgca ttttattctg caccagcgga ggttattcta aaattaggaa taatacctgc
    35101 agcaatcggg agggcagtgt ttccaaggtt aagtaacatc aaagacttta aagaatttaa
    35161 acgtaatgta aataaatcat tgcttttaat gtttctaatc tgtttgccgg tgataatcat
    35221 aggcttgtta tattcagggc ttgtattgaa aatatggttt ggtgagaatt accaaattaa
    35281 ttcctttaat atattaaatg tgttattgat cggttttttc ttcaatgcgc tggcaatgat
    35341 accattctct gcaatccagg cattaggaaa atctaaaatt actgctttga ttcattgtgc
    35401 tgaattggtt ccttatttag cccttttgta ttttatggtc gaaaaatatg ggttactggg
    35461 tgccgcaata tcctggagca tacgtgtaat tttagatgct ctgttattac aatggcttta
    35521 tactagaatg tgttctgtat atgaaaacta aaattaacca attactgagt cacttggata
    35581 atgaatagta atatttacgc tgtcattgtg acatataatc ccgaacttaa aaatctgaat
    35641 gcactgatca ctgaactaaa ggagcaaaat tgttacgttg tagtagtcga taatagtact
    35701 aatttcacct tgaaagataa attagcagac attgaaaaag ttcatttaat ttgtcttggt
    35761 aggaatgaag gtatagcaaa agcacaaaat attgggattc gatattctct tgaaaaaggg
    35821 gcagagaaga taattttttt tgatcaagat agccgtattc gcaatgagtt tatcaaaaaa
    35881 ctttcctgct atatggataa tgaaaatgca aaaatagctg gcccagtttt tattgataga
    35941 gataagtcac attattatcc tatttgtaat atcaaaaaaa atggtcttcg agagaaaatt
    36001 catgtcactg aaggacagac accgtttaaa agttcagtaa caatctcatc cggaaccatg
    36061 gtttcaaaag aagtttttga gattgttgga atgatggatg aggaactttt tattgattat
    36121 gtcgatacag aatggtgtct tagatgctta aactatggca tattagttca tatcattcct
    36181 gatatagaaa tggttcatgc tattggggat aagtcagtaa aaatctgtgg gattaacata
    36241 ccaattcact cgccagtacg tcgttattat cgagtaagaa atgcatttct tttgcttaga
    36301 aaaaatcatg tgcctctttt actctctatt agggaagttg ttttttcttt aattcatacg
    36361 actttaatta tcgcaactca aaaaaataaa attgaatata tgaaaaaaca tattttggct
    36421 acgctggatg gaataagagg aattactggg gacggtcgtt ataatgcata gttctgatca
    36481 aaaaagagta gctgtactta tggctacgta taatggcgaa tgctggattg aggaacaatt
    36541 aaaaagtatt attgaacaaa aagatgtaga catctcaatt tttatatcag atgatttatc
    36601 aactgataat acattaaata tttgtgaaga atttcaatta tcatatccct caattataaa
    36661 tatacttcca agcgtcaata agtttggggg ggcaggtaaa aatttttata gattaattaa
    36721 agatgtcgat cttgaaaact atgattatat ctgttttagt gatcaggacg atatttggta
    36781 taaagataaa ataaaaaatg caattgactg cttggtattt aataatgcga attgttattc
    36841 cagcaatgta attgcatatt atccatcagg gcaaaaaaat ttagttgata aggctcagtc
    36901 gcaaactcaa ttcgattatt tttttgaagc agcaggtccg gggtgtacat atgttataaa
    36961 aaaagaaacg cttattgaat ttaagaagtt catcataaat aataaaaatg ctgcacaaga
    37021 tatctgtctg catgattggt ttttatattc atttgcgcgg acgagaaatt attcgtggta
    37081 tattgataga aaaccaacaa tgctgtacag acagcatgaa aacaaccaag tgggagcaaa
    37141 cattagtttt aaagcaaaat acaaacggct tggtttggtg agaaacaagt ggtatcgaaa
    37201 agaagtaact aaaatagcta atgccttagc tgatgatagc tttgtgaata atcaattggg
    37261 taaaggttat ataggcaatt tgatcttggc tttaagtttt tggaagttaa ggcggaaaaa
    37321 ggctgataaa atttatattt tattaatgtt gattttaaac atattttaaa aaacatcacc
    37381 ttacgaatat gaataatata aataaaattt ttataacatt tttatgtatt gaactgatta
    37441 ttggtggtgg tggaagatta ctggagccat tgggaatatt ccctttgcga tatttattat
    37501 ttgtatttag ttttatactt ttaattttta atttagttac attcaatttt tcaatcaccc
    37561 aaaaatgtgt cagtcttttt atatggttgc ttttatttcc tttttatggc ttctttgtcg
    37621 gcttattagc tggtaataaa ataaatgata tactgtttga tgtgcaacca tacctcttta
    37681 tgctgtcact tatatatcta tttacactaa gatatacttt aaaagtattt ttatgtgaga
    37741 tttttattaa aatagttaat gcatttgcat tatatggatc actgttatat atttcataca
    37801 taattttgtt gaatttcggt ttgttaaatt ttaatttaat ttatgaacac ttatcattga
    37861 ctagcgagtt cttttttcgt cccgatgggg cttttttttc caaatccttc tacttttttg
    37921 gtgtcggtgc gattatcagt tttgtcgaca aaaaatattt aaaatgtctc ataatagtgc
    37981 ttgcgatatt attgacagaa tcaagaggtg tattactttt tacaacatta tcactgttat
    38041 tagccagttt taaattacat aagctatatt taaatactat tataataata ttgggcagcg
    38101 ttctatttat aattatgctt tacatggtcg gatcacgcag tgaagattct gactctgtta
    38161 gatttaatga tttatatttt tattataaaa atgttgattt agcgacgttc ttgtttggaa
    38221 gaggatttgg ttcatttata ttagatcgat taaggattga aatagtacct cttgagatac
    38281 ttcagaaaac aggcgttatt ggtgtattta tatcattagt tcctatgttg cttatctttt
    38341 tgaaaggcta ttttttaaac agtacaaaaa catcattaat gatgtcgtta atactttttt
    38401 tcagtattac cgtttctata actaatccat ttctttttac acccatggga atttttatta
    38461 taggcgttgt agttttatgg gtattttcta tagaaaatat ccaaattagt aataacctca
    38521 cttctggagc aaaataaagg atgttaaaaa tagggaagtt attgaccagt tcttttttta
    38581 gttatttttt aattggaatt gtcaatactg ctttacattg gggggtattt tatgcgtgtt
    38641 ataataattt agcttttggt caaggccgaa gtaacattgt tggttttata tgtgcagcaa
    38701 ccttttcttt ttttgcaaat gcaagatgct catttaaagt atctgcaacc aaggctagat
    38761 attttatatt tatatttttt atgggggcga tgagttattt attcggcgtt ttatttgatc
    38821 tcttagcttt atctccaata tttacattgt tcaccttttc tctttttagt ttggttttag
    38881 gatattgcgc atcaaaatat ttcatttttc gttagtaaac gatttgttaa aaacacaatt
    38941 acttgcacat tagctaaata gtttatatga ggggcttatg tatcactctt ttaatatcgc
    39001 tgtaattatt ccttgttata atgagcagaa ggcaatagca aaagtcataa atgatttcaa
    39061 aacgaatatt ccaactgctt ctatatatgt atttgataat aattcgactg acagtacagc
    39121 acaagtggca gaggatgctg gagcaacagt tcactctgtt cctctgaaag gcaaggggaa
    39181 tgtcgtaaga cgtatgttct cagatgtaga tgcggatatt tatctaatgg ttgatggaga
    39241 tgacacttat gatgcaagtt ctgctccaga aatgattaac catctcataa aaaatcaatt
    39301 ggatatggtt gtaggatgtc gtcaagagaa tggagatcaa aatacataca gaaaagggca
    39361 tcgctgagga aataaactgt tgactggtac tgttcagact atctttaaag ggaattttac
    39421 tgatatgttg tcggggtatc gtgtattttc cagacgctat gttaaatctt ttccatgtct
    39481 atcaagggga tttgaaatcg aaactgagct tacaattcat gctttagaat tacgtatgaa
    39541 gtatggtgag gttaatacaa aatatggcga gcgatctgaa gggagtgtga gcaaactatc
    39601 aacttggagt gatgggttca agattctgaa aacaataatt aaactttact cgcttgaacg
    39661 gccactatac ttttttagta ttatcggcgt tttacttgct gctttgtcta taattttagg
    39721 attgcccatc attgttgatt atattgatac aggtttggtg agaagatttc cgactgcctt
    39781 tttaactgcc tccatcatgt tatcgtcaat aatggcattt gtatgtggca ttattttgca
    39841 ttcaaataca acaactagac gtgaaatgaa ggcattattt tatctttctg aaaaaaaact
    39901 ataaactgat aatgtaaata gagtatttta ctatgattga attgccgttg tatttgtatg
    39961 ttgtgtattg ttttgctttt ggaatgacat tttttgtcat tggtaatatt gtattgtcat
    40021 ctttttttaa ggagggggtg gcaggactta acaaattttc tttatttttt tattgcctat
    40081 gcatagggat gtctttaagt attttgataa tttactttct gggcatggtc gggcaattta
    40141 ataagattgc tatcttttta atttttacgg tgtgttgggt tttaagcatt attaaacgtc
    40201 agcaatttag atggctggca ataaataata ttgagttcag tacgctgttt gttattttat
    40261 ttcttgtttt aatttttgcg gttacattgt tatcgtccct gcgagctccc ggtgattggg
    40321 atgatactat gtatcactta cctttggctc gttcattagt tgagcaccat gcaattgttg
    40381 ttgaacaata tttgcgtttt cctctgttcc cgcaaaatgc agatctttta atggctttag
    40441 gtttacagtt aggagatgta aggttagctc agtttttagc aaatatctgt ttttttgtaa
    40501 tagcctgtgg acttgttggc tgttcatggg aaattacgaa aacttattat cttggcatta
    40561 ttgctacaat cctactattt actatcaatc cacttaaaga tcatcttggt tatgcatata
    40621 ttgatttaac gctatctttg ttttgctgca gccagtattc ttatatatat tcgctcagaa
    40681 aacaataaaa cacaaaatga attgattttt ttatgtggtt tttttattgg aattgcttgt
    40741 ggcgtaaaac tatttggtgc catatatgga gccatcatat ttgttatatt gatagcgaaa
    40801 aagagaaaca tacagacatt tcttattttt agcttggcaa caattcttgg tggcatttat
    40861 tggtatgtcc gtagtttttg gatctctggt gatcctttta gtcctgctgg aggaaatata
    40921 tttggtcact acctatggaa tgaaattgat ctacaagttc aaacagcaga acaagctcga
    40981 catggtataa gtcctgccac attagatttg caacaagcaa ttaataaagt tggcagtgat
    41041 gttgtttttt atgctatact gtctgtcttt acacttagga ataataaaat actttgggta
    41101 tttttctcga ttgttttaac atatgtctta ttttggtttt ctgttacgca aggtgacagg
    41161 tatttatcac ccatttaccc attgtcagta ttgctggttg cgatctcaat tgcaagttta
    41221 ataaaagata tcaacattac aaattacaag ttgggacgat attttatcct tgtgtgtatt
    41281 attttttttc ggggcgatcg ttgtattttg aattcaaata tttttattct gaatgcttga
    41341 attttaattc acgcttagaa agtcgctata gctatgaact gatgaagaaa gcaagtgaat
    41401 atagtgagtt atatggagat aatcttatcc aattaggctt ggaggatggt atttacttct
    41461 ataaagggat ggcaattggt gacgtctttg gattagcaag atatagtgat tggactatta
    41521 gtaatcccga gtgtgaggta atcccacagg atgatttaat agagaagatg aaatctttta
    41581 atagttcgtt tattgttata tctaaacgaa gctatgctaa ttttaatcca gaaaagtatc
    41641 caaaattcaa agttcttatg gatacaccca atggcatact tatcgctata aagtaagaag
    41701 catgctgtaa ccatatgtat tccttgatta gaagatgaaa atcacgacgg ttaataatat
    41761 aacggttaat agacggttaa taatatagaa gacaggagta aacaatgtca aagcaacaga
    41821 tcggcgtagt cggtatggca gtgatggggc gcaaccttgc gctcaacatc gaaagtcgtg
    41881 gttataacgt ctctattttc aaccgctccc gcgaaaagac ggaagaagtg attgctgaaa
    41941 atccaggcaa gaaactggct ccttactata cggtgaaaga gtttgttgaa tctctggaaa
    42001 cgcctcgtcg catcctgtta atggtgaaag caggtgcagg cacggatgct gcgattgatt
    42061 ccctgaaacc atacctcgat aaaggtgaca tcatcattga tggtggtaat accttcttcc
    42121 aggacaccat tcgtcgtaat cgtgagcttt cagcagaggg ctttaacttc atcggtaccg
    42181 gtgtttccgg cggtgaagag ggcgcgctga aaggtccttc tattatgcct ggcgggcaga
    42241 aagaggctta tgagctggtt gcgcctatcc tgactaaaat cgctgccgtg gcagaagacg
    42301 gcgagccatg cgttacttat attggtgccg acggcgcagg ccattatgtg aagatggttc
    42361 acaatggtat tgaatacgga gatatgcagc tcattgctga agcgtattct ctgctcaaag
    42421 gtggcctgaa tctatctaat gaagaactgg cccagacttt caccgagtgg aataacggtg
    42481 aacttagcag ctacctgatc gacatcacta aagatatctt caccaaaaaa gatgaagacg
    42541 gtaactacct ggttgatgtg attctggatg aagcagcaaa caaaggcacc ggtaaatgga
    42601 ccagccagag cgcgctggat ctcggcgaac cgctgtcgct gattaccgag tctgtgtttg
    42661 cacgttatat ctcttctctg aaagatcagc gcgttgccgc atctaaagtt ctctctggtc
    42721 cgcaagctca gtcagcaggc gacaaggctg agttcatcga aaaagttcgc agtgcgctgt
    42781 atctgggcaa aatcgtttct tacgctcagg gcttctctca gctgcgtgct gcgtctgaag
    42841 aatacaactg ggatctgaac tacggcgaaa tcgcgaagat tttccgtgct ggctgcatca
    42901 tccgtgcgca gttcctgcag aaaatcaccg atgcttatgc cgaaaatccg cagatcgcta
    42961 acctgctgct ggccccgtac ttcaagcaaa ttgccgatga ctaccagcag gcgctgcgcg
    43021 atgtcgtcgc ttatgcagta cagaacggta tcccggttcc gaccttcgcc gctgcggttg
    43081 cctattacga tagctaccgt gccgcggttc tgcctgcgaa cctgatccag gcacagcgtg
    43141 actatttcgg ggcgcatact tataagcgca ttgataaaga aggtgtgttc catactgaat
    43201 ggctggatta atctgattta accaacaata aaattgaggc ccggcgtata ttgcaccggg
    43261 cttttttttg ccaaatatct tatagactaa atttcactgc ttaatattaa cttaataaac
    43321 atcagctatt cttataaaga aaatctgaat tgtttttcgt tgcgttgacc atctgacaac
    43381 gtagcgttaa aacttttagc atcttatcac gatgctaaaa acatcatgat tcacagttaa
    43441 gttaattctg agagcatgaa atgaaaatca ccatttccgg tactggctat gtaggcttgt
    43501 caaacgggct tctaatcgca caaaatcatg aggttgtggc attagatatt ttaccgtcac
    43561 gcgttgctat gctgaatgat cggatatctc ctattgttga taaggaaatt cagcagtttt
    43621 tgcaatcaga taaaatacac tttaatgcca cattagataa aaatgaagcc taccgggatg
    43681 ctgattatgt catcatcgcc actccaaccg actatgatcc taaaactaat tatttcaata
    43741 catccagtgt agaatcagta attaaagacg tagttgagat aaatccttat gcggttatgg
    43801 tcatcaaatc aacggttccc gttggtttta ccgcagcgat gcataagaaa tatcgtactg
    43861 aaaatattat tttctctccg gaattcctcc gcgaaggtaa agccctttac gataatctcc
    43921 atccgtcacg tattgtcatc ggtgagcgtt cagaacgcgc agaacgtttt gctgcgttgt
    43981 tacaggaagg cgcgattaag caaaatatcc cgaccctgtt taccgactcc actgaagcag
    44041 aagcgattaa acttttcgct aatacctatc tggcgatgcg cgtagcatac tttaatgaac
    44101 tggatagtta tgcagaaagt ttaggtctga atactcgtca gattatcgaa ggcgtttgtc
    44161 tcgatccgcg tattggcaac cattacaaca atccgtcgtt tggttatggt ggttattgtc
    44221 tgccgaaaga taccaagcag ttactggcga actaccagtc tgtgccgaat aacctgatct
    44281 cggcaattgt cgatgcaaac cgcacgcgta aagattttat tgccgatgcc attttgtcac
    44341 gcaaaccgca agtggtgggt atttatcgtc tgattatgaa gagcgggtca gataacttcc
    44401 gtgcgtcttc tattcagggg attatgaagc gtatcaaggc gaaaggcgtt gaagtgatca
    44461 tctacgagcc ggtgatgaaa gaagactcat tcttcaactc tcgcctggaa cgtgatctcg
    44521 ccaccttcaa acaacaagcc gacgtcatta tctccaaccg tatggcagaa gagcttaagg
    44581 atgtggcaga taaggtctac acccgcgatc tctttggcag cgactaacat cttgttatca
    44641 gggctattta cgccctgatt gtcttttgtt acctccgcaa taattcatta tttttatcac
    44701 ttatcctata gcattcacgg ggattatcgc taaactatgc ggacttggaa atttccgtca
    44761 gttagggtaa tgatgagagt agaaaataat aatgtttctg ggcaaaacca tgacccggaa
    44821 cagattgatt tgattgattt actagtgcag ttgtggcgtg gcaagatgac aattatcatt
    44881 tccgtcattg tggctattgc cctggctatt ggttatttgg cagtagcgaa ggagaaatgg
    44941 acgtcaacag caattatcac tcagcccgac gtggggcaaa ttgctggcta taacaatgcc
    45001 atgaatgtta tctatggtca ggctgcaccg aaagtatcgg atttgcagga gacgttaatt
    45061 ggtcgcttca gttctgcctt ctctgcatta gcagaaacgc tggataatca ggaagagcca
    45121 gaaaaactta ccatcgaacc ttctgttaag aaccagcaat taccattgac tgtttcttat
    45181 gttgggcaaa ctgcagaggg cgcacaaatg aagttggccc aatacattca gcaagttgat
    45241 gataaagtga atcaagagct agaaaaggat ctcaaggaca acattgctct gggacggaaa
    45301 aacttgcagg actctttaag aacccaggaa gtggtcgcgc aggagcagaa agatctgcgt
    45361 atccgtcaga ttcaggaagc gttgcagtat gcgaatcagg cgcaggtgac aaagccacag
    45421 gttcagcaga ctgaagatgt gacgcaagat acgttgttcc ttctagggag cgaagcgctg
    45481 gagtcgatga ttaagcatga agcgactcgt ccgttggtgt tctcaccaaa ctactatcag
    45541 acacgtcaaa acctgttgga tattgaaaaa ttaaagtttg atgatcttga tattcatgct
    45601 taccgctatg tgatgaaacc gacgttacct attcgtcgcg atagtccgaa aaaggcaatc
    45661 accttgattc tggcagtgct tctgggcggc atggttggcg cggggattgt gttggggcgt
    45721 aacgctctgc gtaattacaa cgcgaagtaa tattattgtg catttaagag aaacgggcag
    45781 ggtggtgaca ccatgcccgt tttttttgcc ggatgcgatg ctggcgcatc ttatccggcc
    45841 tacgtgtgtt gagataatgt gtaggcacga taagtttgcg catcgggcaa tggctccggg
    45901 tgtgacaaca acatcacacc tgctcagccc gtaactcact gatgccgttt acgcaggttc
    45961 tcaattactg tcgttaaatc cagatcctga tcctgcaaca gcaccagcag gtgatacatc
    46021 aagtcagatg cctcgttggt cagctcaaag cgatcatgta ccgttgctgc cagtgcggtt
    46081 tccacgcctt cttcgcccac tttctgcgca atgcgtttag tgccgctggc atacagtttg
    46141 gcggtgtagg agatttccgg gtcggcatat ttacgttcgg cgagcagttg ttccagttga
    46201 tacaggaaca gccactggtg agcggtgttg ccgaagcagc tactggtgcc tttgtggcag
    46261 gttggcccga tggaattcgc cagcaccagt aatgtgtcgt tgtcgcagtc cggggcaata
    46321 cttaccacat tgagaaaatt gcccgaggtt tcgcctttgg tccacagtcg ctgtttagtg
    46381 cgcgagaaga aggtgacttt accgctttcg atggttttgt ctaaggcttc cgggttcatg
    46441 tagcccagca ttaacacttc gccagatacc gcgtgttgca caatcaccgg catcagaccg
    46501 tcggtttttt cccagtccag ttcgcgacgt tgttgttctg ttaacatatc ctgatctcca
    46561 cgccctgtgt tgccaggtac gcttttaatt cgccaatatt gattatttgt ttgtggaata
    46621 cggaagctgc cagcgcaccg tcaacgtcgg catcgcggaa ggcttccagg aagtgttcca
    46681 tggtgcccgc gccaccggag gcaatcagcg ggacgtggca aacttcacgc acttttttca
    46741 gctgcttgag gtcgtaaccg ttacgcacgc cgtcctgatt catcatgttg aggacgattt
    46801 ctccggcgcc gcgtttttgc acctcctcta cccagtcgag cgtttcccat tgagtgacgc
    46861 gggtgcggct ttcatcgccg gtatattgat ttacatgata tttaccggtt tcggcgtcgt
    46921 accaggtatc aataccaacc acaatacact gcacgccaaa gcgatcggcc aggcgagtaa
    46981 ttaatgttgg gtctgccagc gcaggggagt tgatggaaat tttatccgcg ccaaaggaaa
    47041 gaattttcgc ggcatcttcc agagacttaa tcccacccgc cacacaaaac ggaatgtcga
    47101 tcacttccgc cacgcgagat acccagcttt tatctaccac acggccatcg ctggaagcgg
    47161 tgatatcgta gaacaccagt tcatctgcac cttcttcggc gtagcgtttt gccagtggca
    47221 cgatatcgcc aatgatttca tggttgcgaa actgtacgcc tttcaccacc tgaccatcac
    47281 gaacgtcgag acacgggatt atgcgttttg ccagcatgcg atggcctcct tcacggtgaa
    47341 tttaccttcc agtaatgcac gaccaactat tacgccgcgc acgccagtgc cacgtagggc
    47401 agcaacatcg ttaatgtcgc caatgccgcc ggaggactga aacgccactt gcggatatct
    47461 ggcacacact tcttcatata aagagacgtt agatcccgcc agcgtgccgt cgcgcgagat
    47521 atcggtacac aacacatgtt tcaggccgac gggcagatag gtttccacca gttgttccag
    47581 tgaaacgccc gagttctctt gccagccgct gactgccact tgcttgttgc cttgctcgtc
    47641 aatacggaca tccagtgcca gcaccagcgc atcggcaccg aaacgttcaa accagccttt
    47701 cactacgtcc ggtgatttca ctgcggtgga accgaccacc acgcgcgcaa cgccagcttc
    47761 cagtaacgcc gccacgtctt cttcactacg aacaccgccg ccaacctgca ccgggacgtt
    47821 aacgcccgcg accagggttt taatcagcgg gatttgacgc ttagccggat cttttgcccc
    47881 ggtcagatcc accaggtgca acacttcggc accttgcgca gcgtaatcct gtaagcgcgg
    47941 cagcgggtcg ttaccgtaat cgcgttgttt gccgtaatcg ccctgatgga gacgcaccac
    48001 ggtgccgtcg attaaatcta atgccggaat aatcatcaca tctccaggaa gtttttcagc
    48061 aacttagcgc cagcggcacc agaacgctcc gggtggaact gcacgccgta gaagttatct
    48121 ttttgtaccg ccgcggtgaa cggttcgccg taattacact gggcgatggt ccacggattg
    48181 actggcattg cgtagctgtg aacaaagtaa aagtacgcgc cgtcttcaat accctgaaac
    48241 aggcggttgc ctgcctgcgg ataaacgcgg ttccagccca tatgtggcag tggcagacca
    48301 aagtcggtca ttttcggcac gtcttcgtcg atgatgccca gcaagtcgac gccattgctc
    48361 tcttcgctgt gtcgtcccag gagttgcatc cctaagcaga tgcccagcac cggttgggta
    48421 caggctttga tgagatcaaa cagctcgcgc tcacgcacct gatccatcgc tgcttgtgcg
    48481 gtgccgacgc cgggtaaaaa cagtttatcg gccagcaaca caacgtccgg gtcacggctg
    48541 actttgggtt cataaccgtg gcgcgcaatg gcagacttca cagagttcag gttggcgcag
    48601 ccagtatcaa ggatcaccac gttcattaca gcactccttt cgacgagggc agggtgtcgc
    48661 cttcgacgcg gatggcctgg cgcagggtac gaccaaaggc tttgaacagg ctttctacac
    48721 ggtgatgatc gtttttacct ttggttttca ggtgtagtgt cacgcccatg gtgtaagaga
    48781 gcgaacggaa gaagtgttcg atcatttcag tgctgagatc gcccacacgc tggtaggtaa
    48841 actcggcttt atattccagg tgcgggcgac cagagatatc cagtgcgcag cgggcaaggc
    48901 attcgtccat cggcagcaca aaaccaaagc ggcaaatacc gcgtttgtcg ccgagggcaa
    48961 tttttaacgc ttcgcccagc gccaggccgg tatcttcgac ggtgtggtga tcgtcgatat
    49021 agaggtcgcc tttgacattg atttccatgc ggaaaccgcc gtgggtggcg atctgatcca
    49081 gcatgtgatc aaagaagcca acgccggtgt taatcttgct gccaccttcg cgatccagcc
    49141 acacctggac gtcaatctgc gtctctttgg tgttgcgcac tacatgggcg taacggtctc
    49201 gtttagtgag ttgctcgcca atcattggcc agttcagggt ttcgcggtcg tagcgtaaac
    49261 cattaatgcc catgttttcc gccagttgaa tgtcggtcgc gcgatcgcca attacataac
    49321 tgttggcacg atccatcgct tgctcagcca gataaccctc caccagtttt actttcggct
    49381 tacggcagtc gcactcatcg gcgggcaggt gcggacaaat cagcacttca tcaaactgca
    49441 cgccttgcga ggtgaagatc tgcatcatca ggttgtgcgg gccatcaaaa tccgcttgcg
    49501 ggaaactttg tgttcccaga ccatcctgat tagtgatcat caccagcttg tagcccgctt
    49561 tttgcagctt cagcaattcc gggatcacgc ccggttcaaa ggcgagtttg tcaaaacggt
    49621 ccacctgaaa atcactcggc ggttcgctaa tcagggttcc atcgcgatcg ataaaaagat
    49681 acttctgact catcaaactt gctccgcacg taaggcgtca atgacgcgct ggctttcttc
    49741 acgggttccg acggtaattc gcaggcagcc gcttaaagag ggttgtttat tctgatcacg
    49801 taagataatg ccctgatccc acaaagattt aaacactgca ctggaggctt taaagcgcgc
    49861 cagaatatag ttggtttcag agtcgaaaac ctgctccacg caggggatct ctttcagtgc
    49921 ggcaatcagg tattcgcgtt ctgtaataat ttgcgccacc cgttcgcgca tggcgacgat
    49981 tccctgcggg cttaacgcct gtgcggcaat gtcggcaacc ggcgtcgaga gcgggtaggg
    50041 ggggatcact ttcatcagca ggttgatgac ttcttcgttt gccagcgtaa atccgcaacg
    50101 aagccccgcc aacgcgaagg ctttcgaaag ggtacgcaaa atcgccaggt gcggatattc
    50161 cgccagccag ccagctaacg atgcctgcgg gcaaaactcg atataagctt catcggcaac
    50221 cacaatcgcc ttaccgcggg ttagctccag cagggggcga aaatcctgcg gattgatcag
    50281 ttgcccggtg gggttgttgg ggctgcaaac atagaccact tttacgccgt ccagcttgtc
    50341 ggaaatgccc tgtaagtcca gttgccagtt ttccagcgtc ggcactgtgc ggcattcgac
    50401 accaatggtt tcagcgctga cgctgtacat gccgtacgtt ggcgggcagt agaggatggc
    50461 gtctttaccc ggttcgcaaa aagcgcggat cagcagttca ataccttcgt ccgcgccacg
    50521 gctgaccagc acctgttccg gttttacgcc tgcatattgc gcgtaatttt caatcaccgc
    50581 ttttggctgg cattccgggt agcggttgag cgtttgctga gtaagctgaa actccacggc
    50641 tgtgggatat tcgttggcgt tcagccagac gtcgccgtta ccgcccagac gacgcgccga
    50701 ctgatacggc gtcaggttgc ggacgttttc acgcgctaaa tcagtaatag tcacggtgct
    50761 catgcttgct ccttaaggac gttaacacgc aaagtaacgg catttttgtg ggcggtcagg
    50821 cgctcggcgg cggccagtgt ttcaatggtc gaagccagcg cggagaaacc ttctttcgac
    50881 agttcctgta cggtcatgcg tttctggaaa tctgccagcc cgaggctgga acaggtggcg
    50941 gtgtaaccgt aagtcggcag cacgtggttg gtgccggagg cgtagtcgcc tgccgattcc
    51001 ggtgaccagt caccaagaaa taccgaaccg gcgctggtga tgccatcgac cagttcgcgg
    51061 gcgttgcggg tctgaatgat caggtgctcc gggccgtact ggttggagat ctccacgcac
    51121 tgcgctaaat ctttagtcac gatcaggcgg ctggcgttca gtgcctggcg ggtggtttcg
    51181 gcacgcggca gttctgccag ttggcgttcg acggcctcgg caacgcgacg cgccatatca
    51241 gcatcggggg tcagtaaaat tacctgtgag tcagggccgt gttcagcctg agaaagcaaa
    51301 tcagaagcca caaaatccgg cgtagcgccg ctgtcagcaa tcaccagcac ttccgacggg
    51361 cctgcgggca tatcgatcgc cgcaccgtcc agacgctggc ttacctggcg ttttgcttcg
    51421 gtgacaaagg cgttacccgg cccgaagatt ttgtccactt tcggcacaga ttccgtacca
    51481 aacgccagcg cggcaatggc ctgtgcgccg ccgacgttaa acacgtcctg cacatcgcac
    51541 agctgcgcca cataaagaat ttcatcggca atcggcggcg gtgagcacag caccactttt
    51601 ttacagcccg caatacgcgc cggagttgcc agcattaata ccgttgagaa gagcggggcg
    51661 gagccgcccg gaatatacaa acccactgaa gccaccgggc gcgtgacctg ctggcaacgc
    51721 acgcctggct gcgtttctac atctaccggc ggcagttttt gcgcagtgtg gaaggtttca
    51781 atactcttta ctgccaccgc catcgcctgt tttagctcgt cgctcaggcg ttcgctggcg
    51841 gcggcgatct catcagcaga caccttcagc gcggtaaccg tggttttatc aaacttcgcg
    51901 ctgtattcac gcagggcctc atcgccacgc gttttcacgt tatcgagaat atcgttaacg
    51961 gtgcgggtaa tgctttcaga agcggagatc gccgggcgca ttaacagctg gcgttgttgc
    52021 tccgcagtac agctattcca gtcaatgatt gtgttaaagc tcatggcgat cactccatca
    52081 tcttctcaat cggcaggacc aggattgaac tggcacccag tgctttcagt ttttccatcg
    52141 tttcccagaa cagggtttcg ctactcacca tgtgcatcgc tacgcgctgt tgatcacccg
    52201 ccagcggcag aatggttggg cgttcggcac ctggcagcag ggcgatgact tcatccaggc
    52261 gttcggtcgg tgcgtgcatc atgatgtatt ttgattcgcg cgcctggatc acaccctgaa
    52321 tacgggtcag tagcttgtcg atcagttgct gtttagattc ttccatttcg ccatcgcgct
    52381 ggatcaggca ggctttcgag cggtagataa cttcgacttc gcgcaggccg ttagcttcca
    52441 gcgtggcacc ggtggaaacc agatcgcaaa tcgcatccgc cagcccggca cgcggcgcaa
    52501 cttcgacaga accgttcagt aagcaggatt taaaagagat acctttctgg tcgagataac
    52561 gcttgagcag gtgaggataa gaggtggcga tacgtttacc gtttaaggag agcggaccgt
    52621 cccaggcttc atcaaccggc gttgccagcg aaaggcggca gccgccgaaa tccagacgac
    52681 gcagggtaaa gtagcgcgga tcttcaccct gggcgcggcg gttaagcagc tcttcttcca
    52741 gcacgttttc gccgataatc ccaaggtcta ccacgccgtc cattaccaga ccgggaatgt
    52801 catcgtcacg cacgcgcaga atatcaatcg gcatgttttc tgccatcgcg atcaggcgct
    52861 gggtgtgaag attgatttta atgccacagc gcgccagtaa ttcgcgtgag tcatcactta
    52921 aacggccgga tttctgcata gctatgcgta aacgagtgtt gtctgtcatt ttcttattcc
    52981 tctttaaacc tgtctgaacc gttaacaacc gcgcaataaa aaagcccccg gaaggtgatc
    53041 ttccgggggc tttctcatgc gttcatgcac cactggaaga tctgaatgtc ttccagcaca
    53101 catcgcctga aagactagtc aggatgatgg tgatgatggt ggtgtttaaa ttgaacgcgt
    53161 gtcataaaaa tctctgtgaa tgtttattca actgatgtct tttaacctaa accactttca
    53221 cgttagaaag caagggcttt tttatatatt ttatggcgat ggatttgttt aatgaatgag
    53281 cggggaaagg agggggctaa gaggactgtc ctctaacttg acagcaccaa aaagagcaaa
    53341 gtctgatttt tcgtctcgct ggcaatgtct tgatctacaa actaattaat aaatagttaa
    53401 ttaacaccta tcattgtaca atgaactgta caaaatagga gatttacatg cgtacaatta
    53461 gctacagcga agcgcgtcag aatttgtcgg caacaatgat gaaagccgtt gaagatcatg
    53521 ccccgatcct cattactcgt cagaatggag aggcttgtgt tctgatgtca ctcgaagaat
    53581 acaactcgct ggaagagacg gcttatctac tgcgttcccc cgctaacgcc cggagattga
    53641 tggactcaat cgatagcctg aaatcaggca aaggaacgga aaaggacatt attgagtgaa
    53701 actaatctgg tctgaggaat catgggatga ttatctgtac tggcaggaaa cagataagcg
    53761 aattgttaaa aagatcaatg aacttatcaa agatacccgc agaacaccat ttgaaggtaa
    53821 ggggaaacca gaacccctga aacataattt gtcaggtttc tggtcccgac gcattacaga
    53881 ggagcaccgt ctggtatacg cggttactga tgattcactg ctcattgcag catgtcgtta
    53941 tcattattga acagattcat tcatagaatg aattgtcagt tttgatacgc tagcgtagcc
    54001 ttatagatct aaggtgtatc aggagataac ggatgaaaaa ggtcgcaatt gtcgggttag
    54061 ggtggttagg tatgccgctg gcgatgtcac tttcagcgcg aggctggcaa gtcaccggga
    54121 gtaaaaccac acaagatggt gtcgaagcgg cccgaatgag tggcattgat agctatctgc
    54181 ttcgcatgga gcctgagtta gtttgcgatt ctgacgatct ggatgccctt atggatgccg
    54241 atgcgctggt cattacgctt ccggcacgtc gtagcggccc cggcgatgag ttctatttac
    54301 aagcggtaca agagttagtg gatagcgcgc tggcccatcg tattccccga attattttta
    54361 ccagctcaac atctgtctat ggcgacgcgc aaggcacggt taaagaaact accccgcgta
    54421 atccggtaac caacagtgga cgagtattag aagaactcga agactggctg cacaatttac
    54481 ccggtacttc ggtcgatatt ctgcgtcttg cgggcctggt cggaccggga cgtcatccgg
    54541 gacgcttctt tgccggaaaa accgcccctg atggtgaaca tggtgttaat ttagtccatt
    54601 tagaagatgt tattggcgct atcactctgt tgttacaggc acctaaaggc ggacacatct
    54661 ataatatatg tgcgccagct caccctgcgc gtaatgtttt ctatccgcag atggcccgtt
    54721 tactggggct gcaacctccg cagttcagaa atagtctgga cagcggcaaa ggtaggatta
    54781 ttgatggcag tcggatttgt aatgaactgg gatttgaata ccagtatccc gatccgctgg
    54841 taatgccgct ggagtaaacg atcacgccag caggacgccg catgcactgc aagggggcgt
    54901 gtatgaaacc actgctggat gtgttgatga tcctcgatgc catagaaaaa gagggcagtt
    54961 ttgcggcggc gtcagccaaa ctctacaaga cgccgtccgc tctgagttac accgttcaca
    55021 agctggaaag cgacctaaat attcaattgc ttgatcgtag cggacaccgc gctaaattca
    55081 cccgcaccgg aaaaatgtta ttagagaaag ggcgggaagt tctgcatacc gtgcgagaac
    55141 tggaaaagca ggcgatcaaa ctgcatgaag gctgggaaaa cgagctggtg attggcgtgg
    55201 acgacacgtt tcctttttct ctgcttgcgc ctctcattga agctttttat caacatcata
    55261 gcgtgacgcg cctgaagttt atcaatggcg tactcggtgg ttcctgggat gccctgactc
    55321 aggggcgagc ggatattatc gttggcgcga tgcatgagcc accatcttcc agtgagtttg
    55381 gtttctcgcg gttgggcgat cttgaacaag tctttgcggt cgcgcctcat catccgttag
    55441 cccaggaaga agaaccgtta aaccgtcgta tcattaaacg atatcgggcg attgtggtgg
    55501 gggacactgc gcaggcaggc gcttctacgg cctcacaact tctcgacgaa caagaagcca
    55561 ttaccgtttt cgattttaaa accaaactgg agctgcaaat tagcggcctt ggctgcggct
    55621 atttaccacg gtatctggcg caacgttttc tcgatagtgg cgcgttaatc gagaagaaag
    55681 tggtcgccca aactctcttt gaacctgtct ggattggctg gaatgaacag accgcagggc
    55741 ttgccagcgg ctggtggcga gatgaaattt tagcaaatag tgcgatcgcc ggtgtttatg
    55801 caaaatctga tgacggaaaa tcagccattt aaagaaaaat tattatgaca agcctctcat
    55861 tctcttgtca tttccccccc atttaggcac aatgcgccgc tgtcaaaaaa tgactaaaaa
    55921 ccgacgttta attagcgtcg gttatttttt gcttcaaacc aatcattcat accaagaggc
    55981 cgggcttcgt accggataga tatttactaa aaatcgacag ttgttgtcgc tgaggaatcc
    56041 aaaaaaatgg ggcaattttt tgcttacgcg acggttatca ccgtaaagga gaataaccat
    56101 gtcgcataac gttactccaa acacctctcg cgtggaattg cgtaaaacgc ttacgttagt
    56161 tccggttgta atgatgggtc tagcctatat gcagccgatg acgctgtttg atacatttgg
    56221 tatcgtttca ggcctcacgg atggtcatgt gccgacagcc tatgcgttcg cattgattgc
    56281 gatcctgttt acggctctga gctacgggaa gctggttcgc cgctatcctt ctgctggctc
    56341 tgcatacact tacgcccaga aatccattag cccgactgtc ggctttatgg tgggttggtc
    56401 ttctctgctc gactatctgt tcgcgccgat gatcaacatt ctgctggcga aaatctattt
    56461 tgaagctctg gtgccttcca ttccatcgtg gatgtttgtg gtggcgctgg tggcctttat
    56521 gaccgccttt aacctgcgta gtctgaaatc cgtagcgaac ttcaacactg taatcgtcgt
    56581 gttgcaggta gtgctgatcg cggtgattct gggcatggtt gtttatggcg tatttgaagg
    56641 tgaaggcgct ggtacgctgg cgagcacgcg tccgttctgg tctggcgatg cgcatgttat
    56701 cccgatgatt accggggcga caatcctgtg cttctccttt accggctttg acggcatcag
    56761 taatctgtcg gaagaaacca aagatgcaga gcgcgtgatc ccgcgtgcga ttttcctgac
    56821 cgcgctgatt ggcggcatga tcttcatctt tgcaacttac ttcctgcagc tgtacttccc
    56881 ggatatctct cgctttaaag atccggatgc gtcacagcct gaaatcatgc tgtacgttgc
    56941 gggtaaagcg ttccaggttg gcgcgctgat cttctccacc attaccgtac tggcgtccgg
    57001 tatggcggcg cacgcaggcg tagcgcgtct gatgtacgta atgggtcgtg acggcgtgtt
    57061 cccgaaaagc ttcttcggtt atgtacaccc gaaatggcgt actccggcga tgaacatcat
    57121 cctggttggc gcgattgccc tgctggcaat caacttcgac ctggtaatgg caacggcgct
    57181 gattaacttt ggtgcgctgg tggcgttcac ctttgttaac ctgtcggtca tctcgcagtt
    57241 ctggatccgt gagaagcgta acaagacgct gaaagatcac ttccagtatc tgttcctgcc
    57301 gatgtgtggt gcgctgaccg ttggtgcgct gtgggttaac ctggaagaaa gctcaatggt
    57361 tctgggtctg atctgggctg ctatcggtct gatttaccta gcttgcgtca ccaagagctt
    57421 ccgtaatccg gttccgcagt acgaagacgt agcataatca tcttttctaa taataaacag
    57481 gagtcgaatg aacatttggc tccggttttt ttattctttt ttatcgaaat cctcattaat
    57541 aaccatttgt tctaatttag agaatttggt tattggtctg cattccacca gtcatgaaaa
    57601 atttccccat taacattcat ctggttaatt aatttatgtt ttcaatgata ttaagcgggc
    57661 taatttgtgg tgctctgctg ggatttgtca tgcagcgtgg gcgtttttgc ctgactggtg
    57721 gttttcgtga tatgtatatc gcgaaaaata atcgcatgtt ttacgccttg ctgattgcaa
    57781 tttcggtaca aagcgtgggg gtttttgcgt taattcaggc gggcctactg acttacgaag
    57841 ccggggcgtt cccgtggctt ggtactgtta taggtgggta tcttttcggg ctgggaattg
    57901 ttctggcggg tggatgtgcc accgggacct ggtatcgcgc gggtgaggga ttgatcggca
    57961 gttggatcgc gcttttcact tatatggtga tgagtgcggt gatgcgttct ccacacgcca
    58021 gtggtttaaa tcaaaccttg cagcactaca ctactgaaca taactctatt gctgagactt
    58081 ttaatttgtc tgtgtggccg ttggttaccg ttttgctggt gataacgctc tgggtggtga
    58141 tgaaagagtt gaagaagcca aaactcaaag tcgcgacctt accgccgcgc cgaaccggga
    58201 tcgctcatat tctgtttgaa aaacgctggc atccctttgt cactgctgta ctcatcggtt
    58261 tgattgcgct tttagcctgg cccctgagtg aagcaaccgg acgcatgttt gggttaggag
    58321 tcacttcccc aacggccaat attctgcaat ttctggtcgc gggtgacgtg aaatacatta
    58381 actggggcgt tttcctggtg ttagggatct tcgtgggggc atttattgca gccaaagcga
    58441 gccgtgagtt ccgcgttcgc gcagctgatg cacaaacaac attacgtagc gggttaggtg
    58501 gtgtactgat gggcttcggt gccagtattg caggtggttg ctctatcggt aatggactgg
    58561 ttatgactgc aatgatgacc tggcagggct ggattggcct tgtatttatg attctcggag
    58621 tctggactgc gtcctggctt gtgtatgttc gaccgcagcg taaggcgcga ctggcaactg
    58681 ctgcggcaaa ttaagtaagg tgtaaagatg gcgattaaaa agctggatgt ggtgacgcag
    58741 gtctgtccgt ttccactcat agaagcaaaa gcagcactgg cagaaatggc cagcggcgat
    58801 gagttggtaa ttgagtttga ttgcacccag gcaactgaag caatcccaca gtgggcggca
    58861 gaagaaggac atgccatcac cgattatcag cagattggcg atgcagcgtg gagcatcacc
    58921 gttcaaaaag cctgactcaa cattgtcctc cgccgtacca gcggcggcgg cttcaaatta
    58981 gacgatctct tccgcgtact gccaaagtgc ttttaacagc gccactttct ctttgtcatc
    59041 ggcatattgt tgtaccagca tctgcaattc atcagcataa ccctgcaaaa actctggcgt
    59101 gaagacctgg cggcggtgct ccagccagcg ttgctgctcg gcataatcca gcgtccccgg
    59161 gaagttgcgt gcccgataat tgaacaacaa cttttcaatt cgtttatcaa caaaagtgat
    59221 atccagtgcc ggtaaattac gcggctcggt ttccagcaca attttcattg ctgcacgatc
    59281 ggcgtcactg aaaaagccat tataaagctg tgcatccaca ttatctgaag gtgtaaacgg
    59341 ttcggcttcc gcgaatatcg ctaccacttt ttcacgcact tgcggatttt cacgcagaat
    59401 ttttaggtta tcgaggcaat gctgacgatt aattcccagt cggtcggcat cttccgggcg
    59461 tagcgtattc gcctgggcca gtaccggaca tttattgata tgcaccagct taaccggaac
    59521 ggcggcgtta tcgccaagat cggcttttgc ggtatataaa cgctcgcgca atgtgtcgat
    59581 atccagctcc agtaatggcg aaatgtctcc tgccaaatcc gccataatta cggcattgcg
    59641 attttcagga tgccacgcca gcggtgccac ccagctggta ttgccacgcc atgccccaaa
    59701 cattccggaa acgtgagcca ggggtttcat ctgcggaaca tcaatcaacg ccatcagttt
    59761 gtgtttatta cgatgggtaa agagataatc aaacaggcgt ggctgacgcg tttttaccag
    59821 tttcgccatc gcaatagtgg cgtacacatc agccatcgca tcgtgggcgt tgctatgttc
    59881 aataccattc gctttggtta aatgctcaag gcgaaagctc ggtagaccgt catcattttc
    59941 aggccagttt attccttccg ggcgcagagc ataacaggca cgcataacat ccagtaaatc
    60001 ccagcgcgag ttatcatgct gccagctcca ggcgtaagga tcgtagaaat tacgataaaa
    60061 aacgttgcgt gtgacttcgt cgtcgaaacg cacattgttg tagcccagaa tacaggtctt
    60121 cggtacggta aaaagcgagt gaatacgggc ggcaaacgcg gcttcgtttt ctcctttcgc
    60181 ccgtgcctcc tgcggggtaa taccggtaat taatactgct ccaggccggg gtaaatagtc
    60241 atccgcgggc ttgcagtaaa agacttcggg ttcgccgatg acattgaatt cgtcatcggt
    60301 gcgaatggct gcgaactgtg cagggcgatc taacgcgggg tgcgtgccaa aggtttcgta
    60361 atcgtgaaag aggaaggtag gttgtttatc tgtgtctgtc attgtctagc attgtccact
    60421 gtagttaatt aactgtctgt tataactcga aaaacaaccc ttttttcacc tgagtatgtc
    60481 tacgaacgtc aaatatagtt gtttactgtg cgctgttgtc catgtgatat tgtgtaccag
    60541 atgtgtacca aactattatt tctgagcgta ccaaatatta tttatggcta taagcgacac
    60601 aaaactgcgt actatttatg gtaaaccata ttcgggccca caagaagtgg ctgatgccga
    60661 tggcctcagc gtacgaattt caccgaaggg ggtcatccag ttccagtacc gctatcgctg
    60721 gcatggcaag cctaatcgac ttgggcttgg tcgataccca tccctgtctt tgaaggatgc
    60781 cagacagatc actgctgact tgcgaaagct ctatttctca ggaacggatc cacgcaccta
    60841 ttttgaagag aaggtggaga actccatgac ggtcgcccag tgtctcgact actggttcga
    60901 caactacgtc tctacaactc tcagagaaaa gacccaggca ctttatcgat caacggttat
    60961 gaagcgcatg catgacgcct tccctaatcg tccggcatct tctatcacgg ttaagcaatg
    61021 ggttgacctg cttaccgaag aagaaaaaga taatccacgc cgagcaaggc aggtgctaag
    61081 tcaactaaga tcagcaatta gttggtgcat gcggcgtcag ttgatagata gttgcgcaat
    61141 tatgagcatc cagccaaggg acttcggctc ccgcgctgag gtaggggagc gggtactgtc
    61201 gtatcacgaa ctggctaaga tttggcttgc tattgaaaga agccgtgcgt ctacgtcaaa
    61261 taagctcctt catcagatgc ttatgctgtg gggggcgagg ctctcagagc ttaggctggc
    61321 aacaaagaca gaatttgacc tgctggacaa cgtatggacc gtaccgaaag agcatagcaa
    61381 gatgggtaat gttatccgcc gtccaatctt cgaacaaatt aagccttttc tcgaaaaggc
    61441 catgacaacg tacaatgatg ttcttttccc tggagaagac ataaacaaac cgatcagcat
    61501 cgctgcagcc aaccgattcg taaatagaat aaggggaggg atggatctag gttactggcg
    61561 aacacatgat ttcagaagaa cgcttgttac acggctgtcc gagatgaatg tcgagcctca
    61621 tgttactgag cgaatgctcg gtcatgaact tggcgggata atgtccgtat acaataaaca
    61681 cgactggata gaggctcagc gcaaagcgta tgagcttcac gctgataaat tgttctggca
    61741 catcaggagc atttctgatt aacgccaccg ttaagaatcc acccttcaac agc
//
LOCUS       DBKEKI010000007        60599 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.007, whole genome shotgun sequence.
ACCESSION   DBKEKI010000007 DBKEKI010000000
VERSION     DBKEKI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.007"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..321
                     /locus_tag="ACUQPX_000424"
     CDS             <1..321
                     /locus_tag="ACUQPX_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9055225.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            434..1228
                     /gene="panB"
                     /locus_tag="ACUQPX_000425"
     CDS             434..1228
                     /gene="panB"
                     /locus_tag="ACUQPX_000425"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HHV9055226.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1240..2091
                     /gene="panC"
                     /locus_tag="ACUQPX_000426"
     CDS             1240..2091
                     /gene="panC"
                     /locus_tag="ACUQPX_000426"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HHV9055227.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVLPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2165..3067)
                     /gene="rpnC"
                     /locus_tag="ACUQPX_000427"
     CDS             complement(2165..3067)
                     /gene="rpnC"
                     /locus_tag="ACUQPX_000427"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HHV9055228.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINFI"
     gene            3341..3721
                     /gene="panD"
                     /locus_tag="ACUQPX_000428"
     CDS             3341..3721
                     /gene="panD"
                     /locus_tag="ACUQPX_000428"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HHV9055229.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3725..4954)
                     /locus_tag="ACUQPX_000429"
     CDS             complement(3725..4954)
                     /locus_tag="ACUQPX_000429"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HHV9055230.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5018..5458)
                     /locus_tag="ACUQPX_000430"
     CDS             complement(5018..5458)
                     /locus_tag="ACUQPX_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHV9055231.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5563..6333)
                     /locus_tag="ACUQPX_000431"
     CDS             complement(5563..6333)
                     /locus_tag="ACUQPX_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9055232.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6330..7256)
                     /locus_tag="ACUQPX_000432"
     CDS             complement(6330..7256)
                     /locus_tag="ACUQPX_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHV9055233.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7365..8027
                     /gene="can"
                     /locus_tag="ACUQPX_000433"
     CDS             7365..8027
                     /gene="can"
                     /locus_tag="ACUQPX_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HHV9055234.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8068..8604)
                     /gene="hpt"
                     /locus_tag="ACUQPX_000434"
     CDS             complement(8068..8604)
                     /gene="hpt"
                     /locus_tag="ACUQPX_000434"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HHV9055235.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8810..11200
                     /gene="gcd"
                     /locus_tag="ACUQPX_000435"
     CDS             8810..11200
                     /gene="gcd"
                     /locus_tag="ACUQPX_000435"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HHV9055236.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11247..12797)
                     /gene="cueO"
                     /locus_tag="ACUQPX_000436"
     CDS             complement(11247..12797)
                     /gene="cueO"
                     /locus_tag="ACUQPX_000436"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HHV9055237.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVRLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12963..13310
                     /locus_tag="ACUQPX_000437"
     CDS             12963..13310
                     /locus_tag="ACUQPX_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HHV9055238.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13416..14282
                     /gene="speE"
                     /locus_tag="ACUQPX_000438"
     CDS             13416..14282
                     /gene="speE"
                     /locus_tag="ACUQPX_000438"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HHV9055239.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVNNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14298..15092
                     /gene="speD"
                     /locus_tag="ACUQPX_000439"
     CDS             14298..15092
                     /gene="speD"
                     /locus_tag="ACUQPX_000439"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HHV9055240.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15130..15492)
                     /gene="yacL"
                     /locus_tag="ACUQPX_000440"
     CDS             complement(15130..15492)
                     /gene="yacL"
                     /locus_tag="ACUQPX_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HHV9055241.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15667..18264)
                     /gene="acnB"
                     /locus_tag="ACUQPX_000441"
     CDS             complement(15667..18264)
                     /gene="acnB"
                     /locus_tag="ACUQPX_000441"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HHV9055242.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18619..20472
                     /locus_tag="ACUQPX_000442"
     CDS             18619..20472
                     /locus_tag="ACUQPX_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HHV9055243.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQASAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRLER"
     gene            complement(20543..21967)
                     /gene="lpdA"
                     /locus_tag="ACUQPX_000443"
     CDS             complement(20543..21967)
                     /gene="lpdA"
                     /locus_tag="ACUQPX_000443"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HHV9055244.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22292..24184)
                     /gene="aceF"
                     /locus_tag="ACUQPX_000444"
     CDS             complement(22292..24184)
                     /gene="aceF"
                     /locus_tag="ACUQPX_000444"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HHV9055245.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24199..26862)
                     /gene="aceE"
                     /locus_tag="ACUQPX_000445"
     CDS             complement(24199..26862)
                     /gene="aceE"
                     /locus_tag="ACUQPX_000445"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HHV9055246.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27023..27787)
                     /gene="pdhR"
                     /locus_tag="ACUQPX_000446"
     CDS             complement(27023..27787)
                     /gene="pdhR"
                     /locus_tag="ACUQPX_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HHV9055247.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28328..29701
                     /gene="aroP"
                     /locus_tag="ACUQPX_000447"
     CDS             28328..29701
                     /gene="aroP"
                     /locus_tag="ACUQPX_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HHV9055248.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29744..30598)
                     /gene="ampE"
                     /locus_tag="ACUQPX_000448"
     CDS             complement(29744..30598)
                     /gene="ampE"
                     /locus_tag="ACUQPX_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HHV9055249.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30595..31146)
                     /gene="ampD"
                     /locus_tag="ACUQPX_000449"
     CDS             complement(30595..31146)
                     /gene="ampD"
                     /locus_tag="ACUQPX_000449"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HHV9055250.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31234..32127
                     /gene="nadC"
                     /locus_tag="ACUQPX_000450"
     CDS             31234..32127
                     /gene="nadC"
                     /locus_tag="ACUQPX_000450"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HHV9055251.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLTENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32330..32770
                     /gene="ppdD"
                     /locus_tag="ACUQPX_000451"
     CDS             32330..32770
                     /gene="ppdD"
                     /locus_tag="ACUQPX_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HHV9055252.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32780..34165
                     /gene="gspE"
                     /locus_tag="ACUQPX_000452"
     CDS             32780..34165
                     /gene="gspE"
                     /locus_tag="ACUQPX_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HHV9055253.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34155..35357
                     /gene="hofC"
                     /locus_tag="ACUQPX_000453"
     CDS             34155..35357
                     /gene="hofC"
                     /locus_tag="ACUQPX_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HHV9055254.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTSELTGKLDECCFELARQQKSQRQLTDKVK
                     SALHYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35392..36435)
                     /gene="guaC"
                     /locus_tag="ACUQPX_000454"
     CDS             complement(35392..36435)
                     /gene="guaC"
                     /locus_tag="ACUQPX_000454"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HHV9055255.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36597..36635)
                     /locus_tag="ACUQPX_000455"
                     /pseudo
     CDS             complement(36597..36635)
                     /locus_tag="ACUQPX_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36660..37280
                     /gene="coaE"
                     /locus_tag="ACUQPX_000456"
     CDS             36660..37280
                     /gene="coaE"
                     /locus_tag="ACUQPX_000456"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HHV9055256.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37280..38023
                     /gene="zapD"
                     /locus_tag="ACUQPX_000457"
     CDS             37280..38023
                     /gene="zapD"
                     /locus_tag="ACUQPX_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HHV9055257.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38033..38230
                     /gene="yacG"
                     /locus_tag="ACUQPX_000458"
     CDS             38033..38230
                     /gene="yacG"
                     /locus_tag="ACUQPX_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HHV9055258.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38330..38719)
                     /gene="mutT"
                     /locus_tag="ACUQPX_000459"
     CDS             complement(38330..38719)
                     /gene="mutT"
                     /locus_tag="ACUQPX_000459"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HHV9055259.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38779..41484)
                     /gene="secA"
                     /locus_tag="ACUQPX_000460"
     CDS             complement(38779..41484)
                     /gene="secA"
                     /locus_tag="ACUQPX_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HHV9055260.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRANKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41546..42058)
                     /gene="secM"
                     /locus_tag="ACUQPX_000461"
     CDS             complement(41546..42058)
                     /gene="secM"
                     /locus_tag="ACUQPX_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HHV9055261.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42289..43206)
                     /gene="lpxC"
                     /locus_tag="ACUQPX_000462"
     CDS             complement(42289..43206)
                     /gene="lpxC"
                     /locus_tag="ACUQPX_000462"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HHV9055262.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43307..44458)
                     /gene="ftsZ"
                     /locus_tag="ACUQPX_000463"
     CDS             complement(43307..44458)
                     /gene="ftsZ"
                     /locus_tag="ACUQPX_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HHV9055263.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44519..45781)
                     /gene="ftsA"
                     /locus_tag="ACUQPX_000464"
     CDS             complement(44519..45781)
                     /gene="ftsA"
                     /locus_tag="ACUQPX_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HHV9055264.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45778..46608)
                     /gene="ftsQ"
                     /locus_tag="ACUQPX_000465"
     CDS             complement(45778..46608)
                     /gene="ftsQ"
                     /locus_tag="ACUQPX_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HHV9055265.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46610..47530)
                     /gene="ddlB"
                     /locus_tag="ACUQPX_000466"
     CDS             complement(46610..47530)
                     /gene="ddlB"
                     /locus_tag="ACUQPX_000466"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HHV9055266.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47523..48998)
                     /gene="murC"
                     /locus_tag="ACUQPX_000467"
     CDS             complement(47523..48998)
                     /gene="murC"
                     /locus_tag="ACUQPX_000467"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HHV9055267.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49052..50119)
                     /gene="murG"
                     /locus_tag="ACUQPX_000468"
     CDS             complement(49052..50119)
                     /gene="murG"
                     /locus_tag="ACUQPX_000468"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HHV9055268.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARIFNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50116..51360)
                     /gene="ftsW"
                     /locus_tag="ACUQPX_000469"
     CDS             complement(50116..51360)
                     /gene="ftsW"
                     /locus_tag="ACUQPX_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HHV9055269.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51360..52676)
                     /gene="murD"
                     /locus_tag="ACUQPX_000470"
     CDS             complement(51360..52676)
                     /gene="murD"
                     /locus_tag="ACUQPX_000470"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HHV9055270.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFACLAKELG"
     gene            complement(52679..53761)
                     /gene="mraY"
                     /locus_tag="ACUQPX_000471"
     CDS             complement(52679..53761)
                     /gene="mraY"
                     /locus_tag="ACUQPX_000471"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HHV9055271.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53755..55113)
                     /gene="murF"
                     /locus_tag="ACUQPX_000472"
     CDS             complement(53755..55113)
                     /gene="murF"
                     /locus_tag="ACUQPX_000472"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HHV9055272.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55110..56597)
                     /gene="murE"
                     /locus_tag="ACUQPX_000473"
     CDS             complement(55110..56597)
                     /gene="murE"
                     /locus_tag="ACUQPX_000473"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HHV9055273.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56584..58350)
                     /gene="ftsI"
                     /locus_tag="ACUQPX_000474"
     CDS             complement(56584..58350)
                     /gene="ftsI"
                     /locus_tag="ACUQPX_000474"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HHV9055274.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGTIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58366..58731)
                     /gene="ftsL"
                     /locus_tag="ACUQPX_000475"
     CDS             complement(58366..58731)
                     /gene="ftsL"
                     /locus_tag="ACUQPX_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HHV9055275.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58728..59669)
                     /gene="rsmH"
                     /locus_tag="ACUQPX_000476"
     CDS             complement(58728..59669)
                     /gene="rsmH"
                     /locus_tag="ACUQPX_000476"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HHV9055276.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59671..60129)
                     /gene="mraZ"
                     /locus_tag="ACUQPX_000477"
     CDS             complement(59671..60129)
                     /gene="mraZ"
                     /locus_tag="ACUQPX_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HHV9055277.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14503 a        16798 c        14906 g        14392 t
ORIGIN      
        1 aaggttggta ctgaaaacgg gcaactcctt ggtaatactc tcacaggtaa tgacgcagca
       61 aaaggagtcg gtgtactcat agaaggttta gcaactagta aaaatcctct aatgacattg
      121 aaacctaatg attcaaattc tgtttataaa gattacgacc cgagaggcaa agacgataca
      181 acaggaggag tttacccgga tcaagataca ggtataacat accctctcca tttccaggcc
      241 acgctacaac aggatggaac tataccaata gaagctggtg agtttaaagc caccagtact
      301 ttccaggtaa cctaccctta ataagtccac cgcaccgcca tcatctggcg gtgcggtaat
      361 tgataaatca ttgcccccac caatgtgact gataaaatgc caggcaacac gacatcattt
      421 atcaggatac gttatgaaac cgaccaccat tgcttcactt cagaaatata agcaggataa
      481 aaagcgtttc gcgaccatca ccgcttacga ctacagcttc gcgaaactat ttgctgatga
      541 agggcttaac gtcatgctgg taggcgactc gctgggcatg accgttcagg gccacgattc
      601 cactctgcca gtaaccgttg ctgatatcgc ctaccacact gccgccgtac gtcgcggcgc
      661 accaaactgc ctgctattgg ctgacctgcc gtttatggcg tatgccacgc cggaacaagc
      721 ctttgaaaac gccgcaacgg ttatgcgtgc cggtgccaat atggtcaaaa ttgagggcgg
      781 tgagtggctg gtcgaaaccg taaaaatgct gaccgaacgt gccgttcctg tatgtggtca
      841 cttaggttta accccacagt cagtgaatat tttcggtggc tacaaagtac aggggcgcgg
      901 caatgaagcg agcgatcgac tactcagcga tgcattagcc ttagaagccg cgggggcaca
      961 gctgctggtg ctggaatgcg tgccggttga gctggcaaaa cgtattaccg aagcactggc
     1021 gatcccggtt attggtattg gtgcaggcaa cgtcactgac gggcaaatcc tcgtgatgca
     1081 cgacgccttt ggtattaccg gcggtcacat tcctaaattc gctaaaaatt tcctcgccga
     1141 aacgggcgac atccgcgcgg ctgtgcggca gtatatggct gaagtggagt ccggcgttta
     1201 tccgggcgaa gaacacagtt tccattaagg agtcacgttg tgttaattat cgaaaccctg
     1261 ccgctgctgc gtcagcaaat tcgccgcctg cgtatggaag gcaagcgcgt ggcgctggtg
     1321 cctactatgg gtaatctgca cgatggccat atgaagctgg tcgacgaagc caaagcccgc
     1381 gccgatgtgg tcgtcgtcag tattttcgtt aacccgatgc agttcgaccg cccggaagat
     1441 ctggcacgtt atccacggac cttgcaggag gactgcgaga agctaaacaa acgtaaagtg
     1501 gatttagttt tcgccccttc ggtaaaagag atctacccga acggtactga aacccacact
     1561 tacgttgacg ttcctggcct ttcgaccatg ctggaaggtg ccagccgtcc gggacatttt
     1621 cgcggcgttt cgactattgt cagcaagctg ttcaacctgg tcctgccgga catcgcctgc
     1681 ttcggtgaaa aagattttca gcaactggcg ttgatccgca aaatggttgc cgatatgggc
     1741 ttcgatattg agattgtcgg tgtgccaatt atgcgcgcca aagacggtct ggcgctaagt
     1801 tcccgtaacg gttatctgac ggcggaacaa cgcaaaattg cgcctggtct gtacaaagtt
     1861 ttaagttcga ttgctgacaa attgcaggct ggggaacggg atctcgatga aattattacc
     1921 attgcggggc aagaactgaa tgaaaaaggc ttccgcgccg atgatattca gattcgcgat
     1981 gccgacacat tgctggaagt ttctgaaacc agcaaacggg cagtaattct ggtagccgcc
     2041 tggcttggcg atgctcgcct gatcgacaac aaaatggtcg agctggcgta atacttaact
     2101 ggcgctacgg ctgatggcgc cagttattaa tttaccccac gtcatctgtc ataacacagg
     2161 taacttaaat aaagttaatt accttatcaa tctcagcaag aggtaaattt gccatctctg
     2221 caacgtcttc ccgagacatt cctttactta gaagacgcag ggcgaattct tgcctttcct
     2281 cctgtcttcc ctgctgaatc cccttctcaa tccctttctc ttcaaaccac tgcgccagcg
     2341 tcatcataga cttccctccc gtttccctgt ctctcagcac accataaaac aaatccgctt
     2401 gttcagtatg accgcgttgc agcatatagt tttgcatggc aactaactga cttccgctag
     2461 tgtacccttc gtcaatcagc gtgaccagtt gctccagcaa taacattaag tcgcgctggc
     2521 gaatatgttt ttgcagtagt tcgagaatcg caatccgccg atgttgcatg atttcgtcat
     2581 ccggtgtgat ggtgatatcc accagcggga aaggactgtt atagacgcgt cgcgccagct
     2641 ccggcgagta aaacatatca aaccagcaca ttgagagcgg ataaggtgtg gcctcgccct
     2701 gataaaacag tatcggcacc accagcggca gcttatcgtg gccagcctcc agatgccggt
     2761 gcatggcagc aatagaataa cgcatcatgc gaaaggccat tttcttatcc ggcttgcttt
     2821 ggtgttcaat cacaacatgc agataaccgg gattgccctg catttgcacg gaatagagca
     2881 cgtccgtgct gtgacctttc aggctctctt caatgaaact ccccgactct aaatgaagcg
     2941 tgttgagatc acaaagttcg cgtaattcca ctggcaaatg tatctccaga aagtcgcgag
     3001 ccgtctccgc atgcattaaa aattgtttaa ataccgcgtc atgcggtgtg gtactcggtg
     3061 catccatctt ccctgtcttc cgtgtggcct tgcgtgaagg cagtatccag tcaaggcgtg
     3121 ctatcgtcag ccacctcttt attgtttacc gagcagcgtt cataaaaata tcaattcgtt
     3181 acaggcgtta catggcacgc ttcggcgcgg tacgcaaact gctgacgcaa atgccatcag
     3241 atgagtagac actcatcaaa aatcgggcaa tactgcgtga gaatttcctc aaagcaggca
     3301 ccgcctgctt cgttaacgac agggtagaaa ggtagaagtt atgattcgca cgatgctgca
     3361 gggcaaactc caccgcgtga aagtgactca tgcggacctg cactatgaag gttcttgcgc
     3421 cattgaccag gattttcttg acgcagccgg tattctcgaa aacgaagcca tcgatatctg
     3481 gaatgtcacc aacggcaagc gtttctccac ttatgctatc gcggcagaac gcggttcgag
     3541 aattatttct gttaacggtg cggcggccca ctgcgccagc gtcggcgata ttgtcatcat
     3601 cgccagcttc gttaccatgc cagatgaaga agctcgcacc tggcgaccca atgtcgccta
     3661 ttttgaaggc gacaatgaaa tgaaacgtac cgcgaaagcg attccggtac aggttgcttg
     3721 attgttatcc ctgcggctgg ttactcacca gccgcgacat cttctccagc gaatccgttc
     3781 ttaagatata aagtcgtttt agtaacaacg gattatcccc cggttttact ttgcctttca
     3841 tggttgtcac cgccaggtga aatccggcat cgtttgctgc cttcacggct ttgtcattaa
     3901 agccgccaaa cggatacgaa agataccaga catgtggatt aaattgcgcc agagcgcggc
     3961 gtgaacgtgc aaaatcaaac agaatattgt gctcactacg gctcagtaat atgggtcggc
     4021 gataaccatc tacccgatgc aaaaaatggg tatgtgactg gaaatcaaaa acatcgcgaa
     4081 tttcgttaag ctcagaaacg ctcataaatt gcagcgattt tgggttccac ttctgcgggt
     4141 gacgtttgat gcgtgaggta acaataaaca ccgtcgcctt catgccatat tgtttcaaca
     4201 caggatacgc atagcggctc accgacttga ggccatcatc aaaggtaatc accaccgctc
     4261 gcgcagggag attgatcgta ttcttcacgt aaccttccag ctgcgccatg ctcagtgtcg
     4321 cgtatcccct gtcgcgcagc caggccattt ggttattgaa agcgcgtacc gaggtggtcg
     4381 tcgaagtatg gcgaaaacgg gtgttttctt cgtcgcgcag aatatggtga taagttagca
     4441 ccggcaggcc attatcgggt tgggcatcca gcgcgctgat ataggccagt cgatcgccaa
     4501 tacggatctg ataccaggtt tgatttaacc tgtctttcag tttatgcaaa atcgggtagc
     4561 gcaaattgtc cgccagtacc ccaaatggcg cactgcccac actcggcgcg ttgtagaccg
     4621 gcgtatcttt ccaggtaatt aagttctgat tactcagcgg cttgttgagg tcacccaaac
     4681 cgtcttcaac tttttgtcgc ccctgaactg gctcgagatg ccctttatcg ataaaccctt
     4741 taccaaagcc aaaattaaat gcgtaataac ttgcggcagt gggctccacg gcaatgattt
     4801 gtccggcccg aatattcccc acggtcacca tcttgtcacc aatttgcgcc cagaccgcag
     4861 cattttcgat ggtttgcata taacgggcag gtaacgcggc actgacactt gcggtaaaca
     4921 gcatcagcag gagaataaca gcttgtttat acatgaaaaa ataaccgagg gtgaaacaac
     4981 gcttttattg tagcaaaagc gccatcagaa tcgcgggtta atactcatac aaattatgtt
     5041 tgaggacgaa aggcggattt ttttgttgtt ggtgccaaag actactcacc tccggcccac
     5101 ccagttcgac aatacgctca cgaaactctg aactggtcat ggtttcacgg caagccatca
     5161 tctgttcaat taacggtaac gtgacaccag aaatcacttc gcaacgggag tgtttgtggc
     5221 ttaataatga agccacgcga tacggtggcg ctccggctat atcggttaag aagatgacac
     5281 cctcaccaga gtccgcttca tgcagagcat cgcacatcat gcggctgagc atattagagc
     5341 ttaatccgcg ccagaaattc acggcccggc actgaagaag tgccccatgt tttttctcca
     5401 gcgcatccag tatctcttgc gcccggtcat cgtgacaggt aattacccaa cctaacatcg
     5461 ccttcctcct tagccgaaaa tcagtctacc ctgaccattg ttgataaagg gtgatagccg
     5521 tcaaaactcc tctcccccga atccagagga gaggaaatag ccttagctac gcaaaccacg
     5581 tccacgttgg atcagcgacc aacagatcaa ataaaacgcc acaataaaga ccaccagtac
     5641 gccaaaggta gtgaccagcg gcacatcatt tataccgagg aagccgtagc ggaaaccact
     5701 gatcatataa acgattgggt tcaggtgcga cagcccttgc cagaacggcg gcaacaaagt
     5761 cagtgagtaa aagaccccgc ccaaatacgt gagtggcgtt aacacaaagg ttggcaccag
     5821 gctgatgtca tcgaacgttt tggcaaacac gccgttcagc aaacccgcaa gggagaatag
     5881 caccgccgtg agcaccaggg ttaaggcaac gaatacccac gaatgcacct gaaacggcac
     5941 aaaaaacagt gaaattgccg tcaccagaat gccaacaaac agaccacgcg ccacgccacc
     6001 gccgacatat ccggcaataa tgacgtgagt cggaaccggc gctaccagca gctcttcaat
     6061 attacgctgg aacttggcac caaaaaatga tgacgcgacg ttagcgtagg cgttggtgat
     6121 caccgacatc atgatcagcc ccggtacgat gaactgcata tagctgaagc catgcatatc
     6181 gccaatacgc gaacctatca ggttaccgaa gataataaag taaagggtca tggtgatgac
     6241 tggcggcacc agcgtctgca cccagatacg cataaagcga tggatctctt tcgcccagat
     6301 gctttttagc gccacccagt aaagatgcat catgcgcgat ctccttgttt ttcattaacc
     6361 agtgaaacaa acagctcttc cagacggtta gctttgttac gcatacttaa tacctgaatg
     6421 ccctgctcac ttaactgcgt aaatacgctg ttgatcccct gctcacgtag cacttcaact
     6481 tccagcgtcg cggtatcgac cagtcgatac tgatagccat cgagcttcgg taacggactt
     6541 ttcggtgcga gatcgagaat aaaggtttcc gatttcagct tcgccagcag cgccttcatc
     6601 gaggtatttt ccaccagctc accatgttga ataatgccga tattgcggca cagcatttct
     6661 gcttcttcca ggtagtgtgt ggtgagaatg atggtggtgc ctttgtcgtt taaatccttc
     6721 aaaaagcccc acattgagcg gcgaagttca atatccacgc ctgcggtcgg ttcgtcgaga
     6781 atcagtagtt taggttcatg cattaacgca cgggcaatca ttaaacggcg cttcatcccg
     6841 ccagataaca tacgcgcacg ttcgttgcgt tttccccata aatcgagctg cttaagatat
     6901 ttttcgctgc ggatgtacgc ctctttgcgc tccacaccgt agtaccctgc ctgattcacc
     6961 acaatttgct gcacggtttc aaacgggttg aagttaaatt cctgcggcac cagtcccaac
     7021 tgacgtttag cgttcacgac atctttctcg agatcgtaac caaatacgct gacccgcccg
     7081 gaggttttat ttaccagaga gctgataata ccgatagtgg tcgatttccc ggccccgttc
     7141 ggcccgagaa gcgcataaaa atcacccgct tcgacctgca aatctattcc acgaagcgcc
     7201 tgaacgccgc ctggatagtt ttttttaagc tgttgaagtt ccagtgcaat ggtcataaat
     7261 ttttacttac cttacgttct tacactttat ctgtggttta aatcgtcccg gagttgccct
     7321 atattagcca aacgtaatta tttggttaca ggtcgttaac ctccatgaaa gacatagata
     7381 cactcatcag caacaatgca ctatggtcaa aaatgctggt ggaagaggat cccgggtttt
     7441 ttgagaaact ggcacaagcg caaaaaccgc gctttctatg gattggatgt tccgacagtc
     7501 gcgttcctgc agaacgttta accggccttg agccgggcga actctttgtt caccgtaatg
     7561 ttgctaacct ggtcattcac accgacctga actgcctttc cgtggttcag tatgcagtgg
     7621 atgtactcga agttgaacac attattatct gtggccacta cggttgcggc ggcgtacaag
     7681 ccgccgttga aaacccggaa ctggggctta tcaacaactg gctgctgcat atccgcgata
     7741 tctggttcaa acatagctca ttgctcggcg aaatgccgca agagcgccgt ctggatacct
     7801 tgtgtgaact gaacgtcatg gaacaggtgt ataacctggg ccactccacc attatgcaat
     7861 cagcgtggaa acgcgggcag aaagttacca ttcacggctg ggcctacggc attcacgacg
     7921 gcttactgcg tgatctggat gttaccgcca ccaaccgcga aacccttgag caacgttacc
     7981 gtcacgggat ttccaacctc aagctgaaac acgccaacca caaataaaaa tgccatgccg
     8041 gatgcaacac atccggcaac ttcacactta ctcgtccagc agaatcactt tgccaatata
     8101 cggcagatga cggtaacgct gtgcgtaatc aatgccgtaa cccaccacaa actcatccgg
     8161 gatcgagaaa ccgataaatt caaccgggac gttcacttca cgacgggacg gtttatccag
     8221 cagcgtacaa atcgccaacg acttcggttc gcgcaggctt aagatctcac gcactttcga
     8281 cagtgtattc cccgagtcga tgatatcttc aacaatcagc acgtccttgc cacggatatc
     8341 ttcatccaga tctttgagga ttttcacatc acgggtggtg gacatgccgc taccgtagct
     8401 ggaggcggtc ataaagtcga cttcatgaga tacctgaact tcacggcaca ggtccgccat
     8461 aaacataaat gagccacgca gcagacccac cagcaccata tcgctgccgc tgtctttgta
     8521 acgctcagta atctgacgac ccagttcggc gatacgcgct ttaatctccg cttcggggat
     8581 cattacttct acagtatgtt tcatatctct aaccatatga tttaaaaaca aatcacttcg
     8641 cacgggcagg ttttggcacc gcaacaaagc gcaagacacg cagtatacca gtaaaactat
     8701 tcattcgcgg aatctgttaa taaagctcga attgtgatga cggtcacaca tgttaaaacc
     8761 tataattaat tgctactaaa atattaatga attgaaatgg tgtctcttta tggcaattaa
     8821 caatacaggc tcgcgacgat tactcgtcac gctaacagcc ctttttgcag cgctttgcgg
     8881 gctgtatcta ctcattggcg gaggctggct ggtcgcgatt ggcggctcct ggtactaccc
     8941 tatcgctggc cttgtgatgc tcggcgtcgc ctggatgctg tggcgcagta aacgcgccgc
     9001 gctttggcta tacgcagccc tgctgctcgg caccatgatt tggggcgtct gggaagttgg
     9061 tttcgacttc tgggcgctga ctccgcgcag cgatattctg gtcttcttcg gcatctggct
     9121 gatcctgccg tttgtctggc gtcgcctggt cattcctgcc agcggcgcag ttgccgcact
     9181 ggtggtcgca ctgctgatta gcggtggtat cctgacctgg gccggattta acgatccgca
     9241 ggagatcaac ggcaccttaa gcgccgatgc cacacctgcc gaagctatct cccccgttgc
     9301 cgatcaggac tggcctgcgt atggccgcaa tcaggaaggt caacgctttt caccactgaa
     9361 gcaaattcac gccgataacg tccacaagct gaaagaagcc tgggtgttcc gtactggcga
     9421 tgtgaagcag ccgaacgatc cgggtgaaat caccaatgaa gtgacgccga ttaaagtggg
     9481 cgataccctt tacctgtgta ccgctcacca gcgcctgttt gcgcttgatg ccgccagcgg
     9541 caaagagaaa tggcattacg atcctgagct gaaaaccaac gagtctttcc agcacgtaac
     9601 ctgccgtggc gtctcttatc atgaagccaa agcagaaacg gcttcgccgg aagtgatggc
     9661 ggattgcccg cgtcgtatca ttcttccagt gaatgatggt cgcctgattg cgattaacgc
     9721 tgaaaacggc aaactgtgcg aaaccttcgc caataaaggc gtgctcaatc tgcaaagcaa
     9781 tatgccagac accaaaccgg gtctgtatga gccgacttct ccgccaatta tcaccgataa
     9841 aaccatcgtg atggccggtt cagtcaccga taacttctca acccgcgaaa cgtctggcgt
     9901 gatccgtggt tttgatgtca acaccggtga gctgctgtgg gcttttgatc cgggcgcgaa
     9961 agatccgaac gcaatcccgt ctgacgaaca cacctttacc tttaactcac caaactcgtg
    10021 ggcaccagcg gcctatgacg cgaagctgga tctggtctat ctgccgatgg gcgtgaccac
    10081 gccggatatc tggggcggta accgcacacc ggaacaggaa cgttatgcca gctcgattct
    10141 ggcgctgaat gccactaccg ggaaactggc gtggagctac cagaccgttc accacgacct
    10201 gtgggacatg gatcttccgg cacagccgac gctggcggac atcaccgtta atggtcagaa
    10261 agtgccgatt atttacgctc cggcgaaaac cggtaacatt tttgtgctcg atcgtcgtaa
    10321 tggcgaactg gtggttccgg ccccggaaaa accggttccc caaggtgctg ccaaaggcga
    10381 ttacgttacc cctactcaac cattctcgga actgagcttc cgtccgacga aagatttgag
    10441 cggtgcggat atgtggggag ccaccatgtt tgaccaactg gtgtgccgcg tgatgttcca
    10501 ccagatgcgc tatgaaggca ttttcacgcc gccatctgaa cagggtacgc tggtcttccc
    10561 gggtaacctg gggatgttcg aatggggcgg gatttccgtt gatccaaatc gtgaagtggc
    10621 aattgccaac ccaatggcac tgccgtttgt ttcgaaactg ctcccgcgtg gtcctggcaa
    10681 cccgatggag cagccgaaag atgccaaagg cacgggtacg gaatccggca ttcagccaca
    10741 gtacggtgta ccgtatggtg tcacgctcaa cccgttcctc tcaccatttg gtctgccatg
    10801 taaacagcca gcatggggtt atatctcggc gctggatctg aaaactaatg aagtggtgtg
    10861 gaagaaacgt attggtacgc cgcaggacag tatgccgttc ccgatgccgg ttccggtgcc
    10921 gttcaatatg ggtatgccga tgctgggcgg gccaatctcc acggcgggta acgtgctgtt
    10981 tatcgccgct acggcagata actacctgcg cgcttacaac atgagcaacg gtgaaaaact
    11041 gtggcagggt cgtttaccgg cgggtggtca ggcaacaccg atgacctatg aagtgaatgg
    11101 caagcagtac gtggtgatct ccgcaggcgg tcacggttca tttggtacga agatgggcga
    11161 ctatattgtg gcttatgccc tgccggatga tgtgaaataa ccataatgcc cggagagatc
    11221 cgggcatatt tccgaatacg gtctttttat accgtaaacc ctaacatcat ccccgtatct
    11281 tcatgctcca gcagatggca gtgcgccata taagcatgtt ctttcggtgc gttgtgatta
    11341 aacttcacca gcacttcact gacattgcct tctaccttaa cggtatcttt ccagcccgcg
    11401 cgatgagccg ctggcggttt gccattttct gacaagatac ggaactgcgt gccgtgaata
    11461 tggaacggat gcagcatcat gtcgcccacg ccagagataa cccaacgttc gtactgccct
    11521 ttcgccgccg caaacatcgg cttattcata tcaaacgcct gaccgttgat tttattggca
    11581 tggtggaaat cgaacttccc gccgtggttc atatgattca tattgccgtg ccccatatgg
    11641 cccatcatct ggctgtgatc cattccggcc atcgcctgat cgccatattt ctccattagc
    11701 atctgcatcc ccatcatatc gagcatcggg tccatagaaa gttgcagctt gcgtaccgtc
    11761 agcccttcca gcgaaggtaa cgcaggcagg ctacttaatg tgtcaggcaa agcaccggag
    11821 gcactaatag caatcggctg aatccgcatt accggatgcg gcttgtcaaa cggtgcaatc
    11881 gccatcccca tctggctgac cggcagcgtc accaggtcaa agggtttgtt gtcgttaacc
    11941 tccaccagca cttcaaaacg ctcgcccatc agcaccggca gctcgctcac cttcaccggt
    12001 tcaggtagca gaccaccgtc gctggcaatc acatacagcg ggcgattgtc gctggtggcg
    12061 aaattgagcg agcgggcatt acagccattg agcaaacgca ggcgcagcca accacgcggg
    12121 gcagcgtgtt gtgggtagat tgcaccgttg gtcagcagcg tatcgccaaa ccagcccacg
    12181 gcggcggtca tcacatccag ttgataatca atctgcccgt cggcgctaaa tttcttatcc
    12241 tgaacgatca ccggaacatc atcgataccc cactgttttg gcagcattaa tttcaggatc
    12301 tcgtcatctt caatcaccac cagaccagcc agccccatcg ccacctgtcg cccggtcttg
    12361 ccatgttgat gtggatggaa ccagcaggta gcggcaggtt gatcaacgtt caacctcacc
    12421 gagcgcttgc cacctggcgg aataattccc tgtgggccgc cgtcgacgtc acccggtact
    12481 tccagcccgt gccagtgtaa cgtcgtctct tccgccagtt ggttgtagat atcaaccgtt
    12541 accgctttgc cgcgctgtaa tttcaccgcc ggccccagca gattgccgtt atagccccag
    12601 gtagttgcag ttttctcgcc aaaggtggac tgacctgcgc caatagttaa ctgaatgcga
    12661 ttacgggcat cggtcgtgag caaatcaggg atcggtaacg ttgggcgttc cgccgcaaag
    12721 actgcacggc tccacagtgg caaggctgaa gccacaccca gtgcgacgga atatttcaag
    12781 aaatcacgac gttgcatagt tatttcctta ttcttaagca ggcgaaacaa aatcaaacgt
    12841 tgagcatagt ccttcccctt acgggaaggt caagccgcag acacaattta atcgacaata
    12901 atggtcgtcg cctcgctcgc agtgtgctaa cgtttatctt ctttaagccc ctggtagaag
    12961 caatgaagac gtttttcaga acagtgttat tcggcagcct gatggccgtt tgcgcaaaca
    13021 gttacgcgct cagcgagtct gaagccgaag atatggccga tttaacggca gtttttgtct
    13081 ttctgaagaa cgattgtggt taccagaact tacctaacgg gcaaattcgt cgcgcactgg
    13141 tctttttcgc ccagcaaaac cagtgggatc tcagtaatta cgacaccttc gacatgaaag
    13201 ctctcggtga agacagctac cgcgatctca gcggcattgg cattcccgtc gctaaaaaat
    13261 gcaaagccct ggcccgcgat tccttaagcc tgcttgccta cgtcaaataa tccctgatac
    13321 ctttttgtag aaatattgac cgtgcatccg cggtcaatgt tagctattat gttgcgccct
    13381 ttttttacgg gtgttaacaa aggaggtatc aacccatggc cgaaaaaaaa cagtggcatg
    13441 aaacgctaca cgaccagttt gggcagtact ttgcggtaaa taacgttctg tatcatgaaa
    13501 agaccgatca ccaggatctg atcatttttg agaacgctgc atttggtcgc gtaatggcgc
    13561 tggatggcgt agtacaaacc accgagcgcg acgagtttat ctatcatgag atgatgaccc
    13621 atgttccgct actggcccac ggtcacgcga aacatgtgct gattatcggc ggcggcgacg
    13681 gtgccatgct gcgtgaagta acccgacata aaaacgttga gtcaatcacg atggtggaaa
    13741 tcgatgcggg tgtcgtgtcg ttctgccgtc agtatctgcc caaccataac gccggtagct
    13801 acgacgatcc gcgctttcaa ctagtgatcg acgatggcgt caatttcgtt aatcaaacca
    13861 gccagacctt tgatgtcatt atctccgact gcaccgatcc tatcggtccc ggcgaaagcc
    13921 tgttcacctc agcattttat gaaggctgca aacgttgcct gaatcctggc ggtatcttcg
    13981 tcgcacaaaa cggcgtctgc tttttacagc aggaagaagc catcgacagc catcgcaaac
    14041 tcagccatta cttcagcgac gttggctttt atcaggcggc gatcccgacc tattacggcg
    14101 gtatcatgac ttttgcatgg gcgacagata acgacgcctt acgccatctc tcaaccgaaa
    14161 ttattcaggc gcgttttctc gcttctggcc tgaaatgccg ttattacaat ccggcaatcc
    14221 atacagcagc ttttgcctta cctcagtatc tgcaagacgc actggcttca cagccgtcct
    14281 aaggagaaga taagaaattg aaaaaactga aactgcatgg ctttaataat ctgaccaaaa
    14341 gtctgagttt ttgtatttac gatatctgct acgccaaaac tgccgaagag cgcgacggtt
    14401 atattgctta tatcgatgaa ctctataatg ccaaccgtct gaccgaaatc ctgtcagaaa
    14461 cctgttccat tatcggggcc aatattctta acatcgcccg ccaggattac gaaccacagg
    14521 gtgccagcgt cactattctg gtgagtgaag aaccggttga cccgaaactc atcgacaaaa
    14581 cagaacaccc cggcccactg ccagaaacgg tggttgctca tctcgataaa agtcatattt
    14641 gcgtacatac ctacccggaa agtcatcctg aaggcggttt atgtaccttc cgcgccgata
    14701 ttgaagtctc tacctgcggc gtgatttctc cgctgaaggc gctgaattac ctgatccacc
    14761 agcttgagtc cgatatcgta accattgatt atcgcgtgcg cggttttact cgcgacatta
    14821 acggtatgaa gcactttatc gaccatgaga ttaattcgat tcagaacttt atgtctgacg
    14881 atatgaaggc gctgtatgac atggtggatg taaacgtcta tcaggaaaat atcttccata
    14941 ccaagatgtt gcttaaagag ttcgacctta agcactacat gttccacacc aaaccggaag
    15001 atttaaccga cagcgagcgc caggaaatta ccgctgcgct gtggaaagaa atgcgcgaga
    15061 tttattacgg gcgcaatatg ccagctgttt aacggctctg gcggagctcc caggctccgc
    15121 cagatttatt tacttctgct gcacgaaatt gcggtaagcc gccacgacct gcagaaaatc
    15181 ctcaacgccg catagcgaca ggctttcttc gtcgtagtag ttcatcccct cttccatttc
    15241 atcgccagcg aattccagtt gattggcgcg aaccatcacc tcttcagcgt ccatccacag
    15301 ggtgtattca tgccctgccc gttgccagga ccgttcgcta cctttcagtg cgtgcgctgc
    15361 ttgttccact tcatcaagca aggccaggtt ttctttcacc tcttcattaa accagtgccc
    15421 gaccacttca tgccccatgg acatacgcac ctttaccact ccggtaatat cgcgcagaaa
    15481 ttcgtaatcc atagtgtgtt cctctgctcc cggcaatgcg ccgaaatcgc atcgcgccat
    15541 tgatgtaatt atcgcagcct taccggagaa aaacagcgga acggcgatgg tttagaaata
    15601 aaaaatgccc gggaatctca ttcaccgggc attgtgtcgt ttatgcgcag cgcgtgcgct
    15661 tacttattac accgcagtct ggaaaatcac cccatcggct ttttcggtgt actgagaaag
    15721 ctggttgaag ttcagataac gataagtatc aacggcagtt ttatctactt gcgcaacgta
    15781 ggtctggtac tcttccggcg tcggcagttt gccaatcagc gccgcaacag ccgccagttc
    15841 tgcagaagcc aggaagacat tcgcgccagt acccaaacgg ttcgggaagt tacgggtaga
    15901 ggtggaaacc accgtcgcac cgtctgctac acgcgcctgg ttacccatac acagggaaca
    15961 gcccgggatc tcgatacgag ccccgctctt accgaagacg ctgtagtagc cttcttccgt
    16021 cagttgcgcg gcgtccatac gggttggcgg tgccacccac aggcgggtcg gcaactgacc
    16081 tttgtgcgca tccagcagtt taccagcggc acggaagtga ccgatgttag tcatgcaaga
    16141 accgataaat acttcgtcga tcttctcgcc ctgtaccgca gacagcggac gtgcgtcatc
    16201 cggatcgttc ggagcacaca ggattggctc tttaatatcc gccagatcga tgtcgatcac
    16261 tgccgcgtat tccgcatctg catcggcttc cagcagctca ggattcgcca gccatttttc
    16321 catgccctga atacggcgtt ccagggtacg acgatcgccg taaccttccg cgatcatcca
    16381 cttcagcagg acgatgttgg agttcaggta ttcgatgatc ggttctttgt tcagcttgat
    16441 agtacaacca gcggcagaac gctcggcgga cgcatcggtt agctcaaagg cctgctcaac
    16501 tttcagatcc ggcagacctt caatttccag aatgcgacca gagaagatgt ttttcttgcc
    16561 tttcttctca acggtcagca gaccttgttt gatcgcatac agcggaatgg cgtgtaccag
    16621 atcgcgcagg gtgatgcccg gctgcatttt gcctttgaag cgcaccagaa cggattccgg
    16681 catatccagc ggcattacgc cagttgcggc agcaaacgcc accagaccag aacccgccgg
    16741 gaaagagata ccgatcggga aacgggtatg ggagtcacca ccggtaccga cggtatccgg
    16801 cagcagcata cggttcagcc aggagtgaat gacgccgtca cccggacgca gcgacacacc
    16861 gccacggttc ataatgaagt ccggcagcgt gtggtgcgtg ttcacgtcaa ctggcttcgg
    16921 atacgccgcg gtgtggcaga aagactgcat caccaggtca gccgagaagc ccaggcacgc
    16981 caggtctttc agttcatcac gggtcatcgg gccggtggtg tcctgagagc ctacagaagt
    17041 cattttcggt tcgcagtacg cgcccggacg aatgcctttc acgccacagg cacggcctac
    17101 cattttctgc gccagcgaga agccgcgatc gctctcagcg acatctttcg cctgacggaa
    17161 cacatcactg tgcggcagac caagtgcttc acgcgctttg gtggtcaggc cacgcccgat
    17221 aatcagcgga atacggccgc cagcacgcac ttcatcaatc agcacgtcgg ttttcagttc
    17281 gaaggtcgcc agcagttcgc ctgtttcgtg gttacgcact tcacctttgt acgggtaaac
    17341 gtcaatcacg tcgcccatgt tcaggttaga gacgttgact tcgattggca gtgcacccgc
    17401 gtcttccatc gtgttaaaga agatgggtgc aattttaccg ccgaggcaca aaccaccgcc
    17461 gcgtttgttc ggcacatgtg gaatatcatc gcccataaac cacagaacgg agttcgtggc
    17521 ggatttacgc gatgaacccg tacccacaac gtcaccgacg tacgccagcg ggaaaccttt
    17581 ctgttgcaga gcttcaatct gtttgatcgg gccaacaaca cctggctggt ctggctcaat
    17641 accttcacgg gcgtttttca gcatcgccag cgcgtgcagt gggatatccg ggcgtgacca
    17701 cgcatccggt gccggagaaa ggtcatcggt gttggtttcg ccagtgactt taaagacggt
    17761 aacggtcagt ttttcagcca gcgccgggcg attcaggaac cattcggcat ccgcccagga
    17821 ctgcattacc tgcttcgcat attcgttgcc tgctttcgct ttctcttcta cgtcatagaa
    17881 gttatcgaac atcagcagcg tgtgagaaag tgctttggca gcaataggtg ccagtttggc
    17941 atcatccagc gcgtcgatca gcggatgaat gttgtaacca ccctgcatgg tgcccagcag
    18001 ttcgatagct ttttccggag tcagcagagg ggatttagct tcgcctttag cgatagcagc
    18061 caggaagcct gcttttacat aggcggcttc atcgacgcct gggggaacac ggttggttaa
    18121 cagatctaac aggaattctt cttcgcccgc gggcgggttt ttcagcagct ctacaagcgc
    18181 ggccatttgg tttgcatcca ggggtttggg cgcaatcccc tcagcggcac gctcagctac
    18241 gtgcttacgg tattcttcta gcacgacggt tctcctcgct ctcattgtca tagtgcggca
    18301 ggcgattctc ttcacgctcc tgtgagacag cagtttgtag ggtaaatgcc cagtaccgca
    18361 acgggcagaa tagcaggatt ttgacgacgt gttaatctgt ttacaaaaaa gcaacataaa
    18421 aaagtggctg aatcgttaag gatggtctag agattgtttc cctccgtaaa aacttcgtta
    18481 attcgcatgg tgataatcac ctttctcaac gaatcccaag attagtcaaa atttaaacta
    18541 ccgcctcttt atactcggat tcacagcacc tgcgggtggc agttcgccga ccattgcgat
    18601 ttccttgaga tccgaattat gaaaatgact ttgccgttta aaccccatgt gctggcgcta
    18661 atttgcagtg ccgggctttg tgctgcctct gccgggctat atataaaaag ccgcacagtg
    18721 gaagcgcctg tagaaccgca atcgacacaa ctggctgtgt ctgacgctgc cgctgttacg
    18781 cttcctgcaa cggtttccgc acctcccgta atacccgcag tcgtcaaatc cgcattcagc
    18841 actgcacaaa tagatcaatg ggtcgcaccc gtcgcgctgt atcccgacgc cctactttcg
    18901 caggtgctga tggcatcaac ctatccgaca aacgttgctc aagcagtgca atggtcgcac
    18961 gataatccac ttaaacaagg cgatgctgct attcaggcgg tatctgacca gccgtgggac
    19021 gccagcgtta aatcactggt ggcctttcca caattgatgg cattgatggg cgaaaacccg
    19081 caatgggtgc aaaacctggg cgatgctttt ctggcgcagc cgcaggacgt gatggactcg
    19141 gtacagcgat tgcggcaact ggcgcaacaa accggctcgc tgaagtcatc aaccgaacag
    19201 aaagttatta ccacaacgaa gaaagctgta ccggtaaaac agacagtcac ggcacccgtc
    19261 ataccatcca ataccgtttt aactgccaac cccgtcatta cagagccggc aacaaccgtc
    19321 atttccattg agcccgccaa tcctgatgtg gtctatattc ccaactacaa cccaaccgtg
    19381 gtttacggga actgggccaa tactgcgtat ccgccggttt atctgccacc accagccgga
    19441 gaaccgtttg ttgacagctt tgtgcgcgga ttcggctata gcatgggcgt tgctaccacg
    19501 tacgcactat tcagcagcat cgactgggat gacgacgatc atgaccatca tcatcatgac
    19561 gatgatgatt atcatcacca cgatggcggt catcgtgaca gtaatggctg gcaacacaac
    19621 ggcgacaaca tcaatatcga cgtcaacaat ttcaaccgta tcaccggtga gcatcttact
    19681 gataagaata tggcatggcg gcacaatcca aactaccgta atggtgtgcc ctatcatgat
    19741 caggatatgg caaagcggtt tcatcaaacc gatgtcaacg gcggaatgag tgccacgcag
    19801 ctacctgctc caacacgcga cagccagcgt caggcggcag caagtcagtt tcagcaacga
    19861 acacacgccg cccccgtcat tacacgagat acccaacgtc aggcatcggc acagcggttt
    19921 aatgaagctg aacactatgg gagctatgac gacttccgcg acttcagccg tcgccaacca
    19981 ctgacccagc aacaaaagga cgccgctcgt cagcgttatc agtcggcctc tcctgagcag
    20041 cgccaggcag tccgcgagaa aatgcagact aacccgcaga tccagcagcg aagggaggcg
    20101 gcgcgccagc gtattcagtc cgcctcgcct gagcagcgcc aggcggttcg cgagaaaatg
    20161 cagactaacc cgcagaacca gcagcgaaga gaggcagcgc gtgagcgtat tcagtctgca
    20221 actcccgaac agcgccaggt gtttaaggaa aaagtacagc agcgcccact gaaccaacag
    20281 caacgtgata acgcccgcca gcgtatacaa tcggcatcac ctgaacaacg tcaggttttt
    20341 cgggagaaag ttcaggagag ccgcccacaa cgtctaaacg acagtaacca tactgtcagg
    20401 ctgaataacg agcaacggtc agcagtacgc gaacgtctct ctgaacgtgg agcaagaaga
    20461 ctggaaaggt aaattgcaga cgtaaaaaaa gcggcgtggt tagccgcttt tttaattgcc
    20521 ggatgttccg gcaaacgaaa aattacttct tcttcgcttt cgggttcggc aggtcggtaa
    20581 tgctaccttc gaacacttct gccgccaggc ccacagactc gtgcagagtc gggtgcgcgt
    20641 ggatggtcag tgcgatgtct tcagcatcac aacccatttc gattgccagg ccgatttcac
    20701 ccagcagctc gccgccgttg gtaccaacaa tcgcaccacc gatcacacgg tgagattctt
    20761 tgtcgaaaat cagcttggtc ataccgtctg cgcagtcaga agcgatagca cgaccagaag
    20821 cagcccacgg gaaggtggcg gtttcatagc tgatgccttt ctctttcgct tctttctcag
    20881 tcagacctac ccatgcaact tctggttcgg tataggcgat ggacgggata actttcggat
    20941 cgaaatagtg tttcttaccg gcgataactt cagcggcaac gtgaccttcg tgaacacctt
    21001 tgtgtgccag catcggctga ccgacgatat cgccgatagc aaagatgtgc ggtacgttgg
    21061 tacgcagctg tttgtcaacg cggatgaaac cacggtcgtc aacttccacg ccagctttgc
    21121 ctgcgtcgag gtttttaccg ttcggcacac gaccaatcgc taccagcacg gcgtcgtaac
    21181 gctgcggttc agctggtgct tttttgcctt ccatcgtcac ataaataccg tcttctttcg
    21241 cttcaacggc ggtaactttg gtttccagca tcaggttgaa tttcttgctg atacgcttgg
    21301 tgaagacttt aacgatgtct ttgtcagctg ccgggataac ctggtcgaac atttcaacca
    21361 cgtcaatctg tgaacccagc gcgtggtaaa cggtgcccat ttccagaccg atgataccgc
    21421 cacccattac cagcaggcgt tctggtactt ctttcagttc cagcgcgtca gtggagtccc
    21481 agatacgcgg atcttcatgc ggaataaacg gcagttggat cgggcgagaa cccgctgcaa
    21541 tgatcgcatt gtcgaagttg atcacggttt tgccgttctc accttcaact tccagggtgt
    21601 tagccccggt gaatttaccc agaccgttga ccactttgac tttgcggcct ttcgccatac
    21661 cagccagacc accggtcagc tgattgatca ctttctcttt ccaggtacga atcttgtcaa
    21721 tatcggtttt cggttcgccg aagacgatac cgtgttcagc cagcgctttg gcttcttcga
    21781 taacttttgc tacgtgcagc agtgctttag aagggataca gccgacgttc aggcaaacac
    21841 cgccaagggt gttgtaacgt tctacgatta cggtttccag acctaaatca gcgcaacgga
    21901 aggcagcgga gtaacctgcg gggcctgccc caagtaccac gacctgagtt ttgatttcag
    21961 tactcatcat gacctctata tatttatctc cggcggtcat acccgtcgtc tttcaggccg
    22021 cagcggcgtt agcttcacgc cctcacccca gccacttact tatgtaagct cctggggatt
    22081 ctggcgcttg ctaccttgct gcacccagaa atccataggg tatgtgaagt aataccctaa
    22141 ccaccaccgg gtcgttctat ccgtcggtat tttacaaaat tgttaacaat ttttaaacaa
    22201 caaacggcaa ccgatttgtc tattcgctaa taacctcaat acattcatga gattaccaga
    22261 aaaaagccgg ccgttgggcc ggctctttta cttacatcac cagacggcga atgtcagaca
    22321 gcgtgttgtt aatgatggta atgaaacggg caccatcagc accgtcgatc acgcggtggt
    22381 cgaaggagag agaaatcggc agcatcagac gcggcacgaa ctctttacca ttccacaccg
    22441 gctccatcgc ggacttggaa acgccgagga tagccacttc cggcgcgttc acaatcggcg
    22501 cgaagtgggt agtacccagg ccgccgatgc tggagatggt gaagcaaccg ccctgcattt
    22561 cgcccgcagt cagcttaccg tcacgcgctt tcttagaaat agtcatcagc tcgcgagaca
    22621 gctcgatgat gcctttcttg ttgacgtctt tgaataccgg aacaaccaga ccgttcgggg
    22681 tatccaccgc cacaccgatg ttgatgtatt tcttcagggt cagacgctga ccgtcttccg
    22741 acagcgaact attgaagcga ggcatctgct caagagctgc agcaacggct ttcatgatga
    22801 agacaaccgg ggtgatcttc acatccagct tacgtttcgc cgcttcttcg ttctgctgtt
    22861 tacggaacgc ttccagctcg gtgatatcgg ttttgtcgaa gtgggtaaca tgcgggatca
    22921 tcacccagtt acggctcagg ttagcaccag aaattttctg gatacggccc agttccactt
    22981 cttcgatttc gccaaacttg ctgaagtcca ccttcggcca cggcagcatg ccagggatac
    23041 caccgccagt cgccgccgga gctgcttctg cacgtttgat agcttctttc acgtaagcct
    23101 gaacgtcttc gcgcaggata cgacctttac ggccagtgcc cttcactttc gccaggttaa
    23161 cgccaaactc gcgtgccaga cggcggatca gcggagtcgc gtgaacgtaa gcgtcgtttt
    23221 cagcaaattc agatttgcct tccgctttcg cagccggagc tgctgccgga gcttcagctt
    23281 ttgctgccgg agccggcgct gccgcttcct gtttcgcagg agctgccgca ggcgctgcgc
    23341 cttcaacttc gaagatcata atcagcgaac cagttttcac tttatcgcca acgttgactt
    23401 tcagttcctt cacgacgcct gcaaacggag ccggaacttc catagaagct ttatcgcctt
    23461 ctacggtaat cagtgactgt tcagcggcaa ctttgtcgcc cactttcacc atcacttcgg
    23521 tcacttcaac ttcgtcaccg ccgatatccg gaacgttaac ttctttcacg ccagctgctg
    23581 gtgcaggggt cgctgccgga gccgcttcct gtttagccgc cggagctgcc gcgcctgctt
    23641 cacccgcgac ttcgaagacc ataatcagcg agccggtaga cactttgtca cccacgttca
    23701 ctttgatctc tttcacggta cctgcaaacg gagccggaac ttccatagaa gccttgtcgc
    23761 cttctacggt gatcagcgac tgttcagctt caactttatc gccaactttc accaggattt
    23821 cggtcacttc aacttcgtcg ctgccgatat ccggaacgtt aacgtctttt gccgccgcag
    23881 ccgcgggtgc tgctgccgga gctgcttctt tcttctcttc tgcctgagca ggtgcagcgt
    23941 ctgctgcacc gtcggcggaa tcgaaaatca taatcagtgc gccggtctgg gttttatcgc
    24001 caacagagac tttgatctct ttaacgatac ccgcctgcgg agatggaact tccatagagg
    24061 ctttgtcgcc ttctacggtg atcagcgact gttcggcttc aactttgtcg cccactttga
    24121 ccaggatctc ggtgatttca acttcatcag ccccgatgtc cggtactttg atttcgatag
    24181 ccattattct tttacctctt acgccagacg cgggttaact ttatctgcat cgatgttgaa
    24241 tttggcgatt gcgtcagcaa ccactttctt atcgatttcg ccacgtttag ccagttcgcc
    24301 cagcgccgca accacgacat aagaagcatc aacttcgaag tggtgacgca ggttctcacg
    24361 gctgtcggaa cgaccgaagc catcagtacc cagtacgcga tagtcgtcag ccggtacgta
    24421 agtacggacc tgctcagcga acagtttcat atagtcggta gatgccactg ccggcgcgtc
    24481 gttcatcacc tgagcgatat acggaacgcg cggagtttcc agcggatgca gcatgttcca
    24541 gcgttcacaa tcctgaccat cacgcgccag ctcggtgaag gaggtcacgc tataaacgtc
    24601 agaacctacg ccgtaatctt tcgccaggat ctctgccgct tcacggacgt gacgcaggat
    24661 ggaaccggag cccagcagct gaactttacc tttgctacct tcaatggttt cgagtttgta
    24721 gataccttta cggatacctt cctcagcacc ttccggcatt gccggcatgt ggtagttttc
    24781 gttcagcgta gtgatgtagt agtaaacgtt ctcttgtttt tcaccgtaca tacgctccag
    24841 accgtcatgc ataatgacag caacttcgta agcgtaagcc gggtcgtaag agatacagtt
    24901 cgggatagtc agcgactgaa tgtggctgtg accatcttcg tgctgcagac cttcaccgtt
    24961 cagggtggta cgaccggaag taccgccgat caggaagcca cgcgcttgct ggtcgccagc
    25021 cgcccagcac agatcgccga tacgctggaa gccgaacatc gagtaataga tgtagaatgg
    25081 gatcatcggc agattgttcg tgctgtaaga ggtcgccgct gccagccagg aacaccctgc
    25141 gcccagctcg ttgatccctt cctgcagaat ctgacctttc tcgtcttctt tatagtaagc
    25201 aacctgctcg cggtcctgcg gggtgtactg ctgaccgttc gggctgtaaa taccaatctg
    25261 acggaacaga ccttccatac cgaaagtacg cgcttcgtcg gcgatgatcg gtaccagacg
    25321 atctttgatc gacttgttct tcagcatcac gttcagagca cgaacgaaag cgatagtggt
    25381 agagatctct ttgctctgct cttccagcag cgcgccgaag tcttgcaggc tcggcagctc
    25441 aagcttctcg gtgaagttcg gctgacggct tggcagataa ccgtgcagtt tctgacgctg
    25501 agcgtgcaga taggtatgct cttcagaacc ttccgggaag gtgatgtacg gcagtttttc
    25561 gatatctgca tcagacaccg gcacattgaa acggtcgcgg atgtgacgca cgccgtccat
    25621 gttcattttc ttaacctggt gcgcgatgtt tttaccttca gccgcgtcgc ccatgccgta
    25681 acctttaatg gtatgagcaa ggattactgt cgctttgcct ttggtttcct gcgctttctt
    25741 gaatgcagcg tagattttct tcggatcgtg accaccacgg ttcagtgccc agatctgctc
    25801 gtcagtccag tctgcaacca gtgctgcggt ttcaggatat ttaccgaaga agtgttcacg
    25861 aacgtacgca ccatctttcg atttgaaggt ctggtagtcg ccgtcaacgg tttcgttcat
    25921 cagctggatc agtttaccgc tggtatcttt acgcagcagt tcatcccaac ggctacccca
    25981 catcactttg atcacgttcc agccagcacc ttcgaagatg ccttccagtt cgttgatgat
    26041 cttgccgtta ccggtgaccg ggccgtcaag acgctgcagg ttacagttga taacgaaaac
    26101 caggttatcc agtttttcac gggtagcgat ggtgatcgca cctttggatt ccggttcgtc
    26161 catttcaccg tcgccgagga acgcgtaaac ggtctgttta gaggtatctt tcaggccacg
    26221 gtgttccaga tatttcagga atttagcctg gtaaatagca ccaatcggac ccagccccat
    26281 agatacggtc gggaactgcc agaattccgg catcagtttc gggtgcggat aggaagagag
    26341 gccattgccg tgaacttcct gacggaagtt atccagctgc tcctgagtca gacgaccttc
    26401 caggaaagca cgggcgtaaa cgcccgggga gatgtggccc tggaagtaaa ccaagtcgcc
    26461 gccatcctgc tcgttgcgtg cacggaagaa gtggttaaag cacacatcat aaatggttgc
    26521 ggaagactgg aaggacgcca tgtggccgcc cagttcgagg tcttttttcg acgcacgcag
    26581 caccgtcatg atggcgttcc agcggatagc tgaacgaata cggcgttcca gttccagatt
    26641 acccggatac tccggttgtt cttcaacggg gatggtgttg atgtagttgc tgatacctgt
    26701 gcctgcggct acgtttacac cgcctttgcg ggcttcagca agcagttggt cgatcagata
    26761 ctgagcacgc tcaacacctt cttcacggat gaccgattcg atcgcctgga gccagtcgcg
    26821 agtttcgatc ggatccacgt catttgggaa acgttctgac atgggttatt ccttatctat
    26881 ctaataacgt tgagttttct ggaacctgtc ccattgaact ctcgccagaa agctcaataa
    26941 gacaggttct acgtttagtt gccgcgcttt tatatgcgct tgatttacaa catcttctgg
    27001 ataattttta ccagaaaaat cactaattct ttcgttgctc cagacgacgc agagaacgct
    27061 cacggcggct ctcttcacgg cttctgtcga gcaaaatttc ttcgataaag gccagatggc
    27121 gatgcgatgc ttcgcgcgct tcttccggct taccggccat aatcgcttca aatatgcggg
    27181 tgcggtgact actcaccagc ggcagcatct cgcgacgcga atagagcaat tcgaagttct
    27241 ggcggacatt ctgggccaac atcggctcca tacaccttag cagatgaagc agaaccacat
    27301 tgtgggccgc ttcggtgacg gcaatctgat actggagtac ggcgtttgat tccgcgtcca
    27361 gatcgccaga ctgctgcgcc agctctatgg cgtggtggag ttcacggatg cgttccttgt
    27421 cttcatcggt actacgcagc gcggcgtaat aagcggcgat accttccaag gcgtgtcgtg
    27481 tttcgagcaa gtcatactgt gattcaggat ggtcggagag cagctccacc agcggatcgc
    27541 tgaagctttg ccataggctg ctctggacaa aagtgccgcc accctggcga cgaagcaaca
    27601 agcccttcgc ttcgagacgt tgaatcgcct cacgcaagga gggacgggag acgtcgaact
    27661 gttttgccag ttcgcgttcc ggtgggagtt tttcgcccgg gcggagagtg ccttcgagga
    27721 tcaaaaactc cagttgctgc tcaatcacat cggagagttt tggttggcgg attttgctgt
    27781 aggccatgag ttcctgtctt aagccacttg ccgaagtcaa ttggtcttac caatttcatg
    27841 tctgtgacgc taaagtaaca aagtattcac cttatgtcca tacaggtttt gattgaaatc
    27901 atgaaactgt gcacatttta acaacttgac atatataacg tttcaaagtt gtaactatgc
    27961 acaaatgtag actttacgta ggaaagggat tttttaacga ttattaaact ataaaaatcc
    28021 gaattgaacc gattcactta ccaatttttt gatttttaag tcaataaaaa cgaatttaaa
    28081 ttcactctgc atattgagag gggttgaggc tgagctttac aaacggtttc tttttaagca
    28141 actcatcttc aaccatgcat aaagcgggtg cattcgctgc cgcataccat tattcttgat
    28201 ctgacggaag tctttttgta acaattcaaa cttccttgat gtaaataaat caatacaaca
    28261 aacggaattg caaacttaca cacgcatcac tgcgtagatc aaaaaaacaa tcaccgcacg
    28321 aggtttcatg atggaaggtc aacagcacgg cgagcagcta aagcgcggcc ttaaaaaccg
    28381 ccatattcag cttatcgcgc tgggtggcgc gatagggaca gggctattcc tgggtagcgc
    28441 ctccgtaata cagtccgcag ggccagggat tatcctgggt tacgccattg ctggttttat
    28501 cgcctttctg atcatgcgtc agctgggtga aatggtggtc gaagaacctg tcgcaggctc
    28561 ctttagccac tttgcttata aatactgggg cagttttgcc ggtttcgcct ctggctggaa
    28621 ctactgggta ctgtacgttt tagttgccat ggcagagctg actgccgtgg gtaaatacat
    28681 tcagttctgg tatccggaaa tcccaacctg ggtttctgcc gccgtattct ttgtggtgat
    28741 taacgccatc aacctgacca acgttaaagt gtttggcgag atggagttct ggtttgccat
    28801 tatcaaagtt attgcggtag tagcgatgat catcttcggc ggctggctgc tgttcagtgg
    28861 caacggcggc ccgcaggcga gcgttagcaa cctgtgggat caaggtggtt tcctgccgca
    28921 cggcttcacc ggactggtga tgatgatggc gattatcatg ttctcgttcg gtggtctgga
    28981 actggtgggg atcaccgcag cagaagctga taacccggag caaagtatcc cgaaagcaac
    29041 taaccaggtt atctaccgca tcctgatttt ctatattggt tcgttagccg ttctgctctc
    29101 actgatgccg tggacccgcg ttaccgccga taccagtccg tttgtgctga tcttccacga
    29161 gttaggcgat acctttgtgg cgaatgcgct gaacatcgtg gtactgactg cggcgctctc
    29221 cgtgtacaac agctgcgtat attgcaacag ccgtatgctg tttggtctgg cacaacaggg
    29281 gaacgcgcca aaagcgctgg cgtctgtcga taaacgcggc gtaccggtta acaccattct
    29341 ggtgtctgcg ctggttacag cattgtgcgt attgattaac tatcttgctc cggaatccgc
    29401 atttggcctg ttaatggcac tggtggtatc cgcactggtg atcaactggg cgatgatcag
    29461 tctggcgcat atgaagttcc gtcgcgccaa gcaggaacaa ggcgtggtaa ctcgcttccc
    29521 tgctctgctt tatccgctgg gtaactggat ctgcctgctg tttatggcgg cggtactggt
    29581 gattatgctg atgaccccag gaatggcgat ttcggtatac ctgatcccgg tatggctgat
    29641 cgtgttaggt atcggctatc tgtttaaaga gaaaaccgcc aaagccgtaa aagcgcatta
    29701 atctctctac gccctcaccc gtacagggtg agggcaataa tctctacacc aacgccccgt
    29761 aaatcgtcag tagcgcaatc accaccacga ccacgaacga ggttttcttc gccattgaaa
    29821 ccgctgcctt cggcgtctcc accttatcga catgcggttc acgcgccaga gagaactgcg
    29881 ccagacgcgt taatacctga tattgcgaag tatggaaatc acccagcgaa gcaaaccagg
    29941 ccggtaacgc tttctcacca tgaccgatca aggcatatac cacacccgca agacgaaccg
    30001 gcacccaatc cagtacatga agcactgcat caatgccgga ctgtaaacga tgatgcggcg
    30061 tctggtatcg cgccagccag tattgccatg cacgcaagaa cgcatacccc atcagcgtaa
    30121 cgggtcccca ggttcccccc acaatcagcc agaacagcgg tgcaagataa aaacgaaagt
    30181 taatccacag caatgcattt tgcagctcac gcaaatactc acgttcgtcg cagcctgccg
    30241 ggacgccgtg aatcatggtg agttcgccag ccatcgtggc acgggcatgg ctatcattac
    30301 gtgaagcagc tgtcagataa gcatgataat gaagacgaac tttacctgcg ccaatacaca
    30361 gcaaaccaat cagcagccac accagcagcg tgggaacgtt gaacaatact ccctgcaatg
    30421 cgcgtaacag taaaaaagtc acacccatcg caataatggt catgcctaac gtgcgcccga
    30481 gagaaaaatg tttcacccgc cgaaagaacg cttcaagacg atgatcaagc tgccagtgct
    30541 cgcccaactt aaacaggcgc tcgaaaatta acaccagtaa ggttgtaaat agcgtcatgt
    30601 tgtctccttg ctgaccagcg cacgaaaccg tgcccaatca aatgcaggac cgggatcggt
    30661 tttccgatcc ggcgcaatat cacaatggcc ggtcatgtta ttagcgatat cgggatagcg
    30721 atctatcagt gcccgcgtaa ccgccgcaag ctgttgatac tgcgcatcgg tatacgccag
    30781 agtatcggtg ccttcaagct caatcccaat agaaaaatca ttgcagcgtt cacgcccctg
    30841 atactgagag actcccgcat gccaggcacg tttatcgaaa ggaacatact ggactatttc
    30901 accatcacgg cgaatcaaac agtgagcgga gacgcgcaaa tgggcgatct cagcaaagaa
    30961 aggatgtgcc tgcggatcaa tagttccagt gaataatgcg tcgatccacg gaccgccaaa
    31021 ctcgcctggc ggcaggctaa tattgtgcac caccagcagg gtgggtgttt cgtcatccag
    31081 gcggcaatcg taatgtggtg agggaacgcg gcgcgcgcca accagccacc cctgttctaa
    31141 caacatgcag gatctcctta tatgtggtgc taatacccgg ttcagagtag catgtttcta
    31201 ccttatgatt cgttagctat ctggagtttt aacatgccgc ctcgccgcta caaccctgac
    31261 acccgacgtg acgagctgct ggaacgcatt aatctcgata tccccggcgc ggtggcccag
    31321 gcgctgcggg aagatttagg cggaacagtc gatgccaaca atgacattac ggcaaaactt
    31381 ttaacggaaa attctcgctc tcatgccacg gtgatcaccc gcgagaatgg cgtattttgt
    31441 ggcaaacgct gggttgaaga ggtgtttatt caactggcag gcgacgatgt caccataatc
    31501 tggcatgtgg atgacggcga tgtcatcaac gccaatcaac ccttgttcga acttgaaggc
    31561 ccatcccgcg tgctgttaac gggcgaacgc actgcgctta attttgtgca aaccctttca
    31621 ggagttgcca gtaaggtacg ccactatgtc gaattactgg aaggcaccaa cacgcagttg
    31681 ttggatacgc gcaaaacctt acctggcctg cgttcagctc tgaaatacgc ggtactttgc
    31741 ggcggcggag cgaatcaccg tctggggctt tctgatgcct tcctgatcaa agaaaaccat
    31801 attattgcct ccggctcagt gcgccaggcg gtcgaaaaag cgtcctggct gcatccggat
    31861 gcgccagtag aagtcgaagt agagaatctg gaagaacttg atgaggccct gaaagccgga
    31921 gccgatatca tcatgctgga taacttcgaa acagaacaga tgcgcgaagc cgtcaaacgc
    31981 accaacggca aggcgctact ggaagtgtct ggcaacgtca ctgacaaaac actgcgcgaa
    32041 tttgccgaaa cgggcgtgga ctttatctcc gtcggtgcgc taactaaaca cgtacaagca
    32101 ctcgaccttt caatgcgttt tcgctaattg ctgacagatt tatccgctcc atcgcgggcg
    32161 gataatctcc ccttccccaa ctttcttcgt aacgcctcgc aaatttcttg tctttcgctg
    32221 caacgaaata aaaaacctcg gaatctgctt tcccgtttcg ttttttgccc ctcgcctgtg
    32281 ctgatggcgt ttgccaaagt agcaccaacc aaatcaagga gcgaaacaga tggacaagca
    32341 acgcggtttt acacttatcg aactgatggt ggttattggc atcattgcca ttttaagcgc
    32401 cattggtatt cccgcttatc aaaactacct gcgcaaagcc gcactcaccg acatgctaca
    32461 aacctttgtg ccttaccgta ccgccgtaga gttgtgcgcg ctggaacatg gtggattaga
    32521 tacctgcgac ggtggcagca atggcattcc ctcgcctacc accacccgct atgtttcagc
    32581 catgagtgtg gcaaagggcg tggtgtcgct gaccgggcaa gaaagtctca atgggctaag
    32641 cgtcgtcatg acgccaggtt gggataacgc aaacggcgtc accggctggg cgcgcaactg
    32701 caatattcaa agtgacagcg cattgcagca agcctgcgaa gatgtcttcc gctttgatga
    32761 cgccaactaa ggagcggcaa tgaatattcc acagctcacg gccctgtgcc tgcgttatca
    32821 gggagtcttg ctggatgcca gcgaagaagt ggttcatgtt gcggtggtcg atgccccctc
    32881 acatgagttg ctggacgcat tgcatttcgc taccaccaaa cgtattgaga tcacctgctg
    32941 gacgcgccaa caaatggaag gtcacgccag tcgcacacaa cagacattgc ccgtagctgt
    33001 tcaggagaag catcagccca aagcagagtt gctgactcga acgttacaat ctgcgctgga
    33061 acaacgcgcg tctgatattc atatcgaacc agcggacaat gcctaccgca tccgcttgcg
    33121 tatcgacggc gtattgcatc ctttaccgga tgtttcaccg gatgccggag tcgcattaac
    33181 agccagatta aaagtgctgg gaaacctgga tattgcggaa catcgcctgc cgcaggacgg
    33241 gcaattcact gtcgaactgg caggaaacac cgtctcattt cgtattgcga ccttaccatg
    33301 tcggggtggt gaaaaggtgg tattaaggtt gttacagcag gtgagccagg cactggatgt
    33361 taacacgctg ggaatgcagc cgttacaact ggcggacttt gctcatgcct tgcaacaacc
    33421 acagggactg gtgctggtaa ctggccctac aggcagcggc aaaacggtca cgctttatag
    33481 tgccctgcaa acgctgaata ccgctgacat taatatttgt agcgtcgaag atccggttga
    33541 gatccccata gccggactaa accagacgca aatccatccg cgtgccgggc tcacctttca
    33601 gggcgttttg cgtgcgttat tgcgccagga tcctgacgtc atcatgatcg gagagatccg
    33661 cgatggcgaa acggcagaaa ttgccattaa agccgcgcaa accggtcacc tggtgttgtc
    33721 cacgctacac accaattcca ccactgaaac gctggtacgt ttacagcaaa tgggggtcgc
    33781 ccgctggatg ctatcatcgg cgcttacgct ggtaatagcc cagcgtctgg tacgtaaact
    33841 ttgcccacat tgtcgccgac agcaagggga gcccatccat attccagaca ctgtatggcc
    33901 gtcgccgctg ccccactggc aggcacccgg ttgtgtacat tgctaccacg gtttttatgg
    33961 tcgtaccgcc ttgtttgaag tcctgcccat tacaccagcc atacgccagc ttatttccgc
    34021 taatactgaa gttgaatcgc tggaaacgca cgcccgacag gcgggtatgc gtacgctttt
    34081 tgaaaacggc tgcctggccg tggagcaagg cttaaccacc tttgaagagt taatccgcgt
    34141 actggggatg ccgcatggca agtaagcaac tctggcgctg gcatggcata accggcgacg
    34201 gcaatgcgca agatgggatg ctatgggcag agagccgtac tttactgctt atggcactac
    34261 agcaacagat ggttacccca ctaagcctga agcgaatcgc catcaattct gcgcagtggc
    34321 gaggagataa aagcgcggaa gtcattcatc aactggcgac gctacttaaa gccgggttaa
    34381 cgctttctga agggctggct ctgctggcgg aacagcatcc cagtaagcaa tggcaagcgt
    34441 tgctgcaatc gctggcgcac gatctcgaac agggcattgc tttttccaat gccttattac
    34501 cctggtcaga ggcatttccg ccgctctatc aggcgatgat ccgcacgagt gaactgaccg
    34561 gtaagctgga tgaatgctgc tttgaactgg cgcgtcagca aaaatcccag cgtcagttga
    34621 ccgacaaagt gaaatcagcg ttgcattatc ccatcatcat tttagcgatg gcaatcatgg
    34681 tggttgtggc aatgctgcat tttgttctgc cggagtttgc cgctatctat aagaccttca
    34741 acaccccact accggcacta acgcagggga tcatgacgct ggcagacttt agtggcgaat
    34801 ggggctggct gctggtgttg ttcggctttc tgctggcgat agccaataag ttgctgatgc
    34861 gccgcccgac ctggcttatc gcgcggcaga aattgctgtt acgcatcccg attatgggtt
    34921 cactgatgag gggacaaaaa ctcacgcaga tctttacgat tctggcgctg acacaaagtg
    34981 caggcattac ttttttacag ggcgtagaga gcgtcagaga aacaatgcgc tgcccgtact
    35041 gggtgcaact tctgacacaa atccagcacg atatcagtaa cggtcatccc atctggctgg
    35101 cgctaaaaaa tgccggtgag tttagcccgc tctgtttgca attagtgaga acaggagagg
    35161 catccggctc gctggacctc atgttagaca acctcgccca tcatcatcgg gataacacaa
    35221 tggcgctggc ggataacctc gcagccttac tggaaccggc gttgctgatc ataacgggag
    35281 gaattatcgg tacgctggtg gtggcaatgt atctgccaat tttccattta ggcgatgcga
    35341 tgagtgggat gggataacgc tggcgtgttg ctccacgcca gcgttgggag attacaggtt
    35401 gttgaagatg cggttttctt gttccagcac acgaataaac gtggtgcgct tggtcagctc
    35461 tttcaggcgt gaagccccaa cgtatgtaca agctgaacgc aggccgccca aaatatcacg
    35521 cgcggtattt tcaaccgggc ctcgcagcgg cagcttaacg gttttacctt ctgctgcgcg
    35581 gtattccgca acgccgccaa cgtgacgttt catcgcagac tcggagctca tgccgtagaa
    35641 cagcataaat ttctcgccgt tctcctcaac gatgcgaccg ccgctctctt cgtggcccgc
    35701 cagcatgccg ccaagcatga cgaaatcggc accgccgcca aaggctttcg ccacatcgcc
    35761 cggcgtggta cagccaccgt cgctgacgat cattccgccc agaccgtgcg ccgcatcggc
    35821 acattcgatt accgcagaaa gctgcggata accgacgcct gttttgacgc gagttgtaca
    35881 aacagaacct gggccaatgc caactttaac gatatcagca ccggagagga taagctcctc
    35941 acacatttca ccagtcacta cgttgccagc acaaatggtt ttggtcggcc acgcttcacg
    36001 cgctttcgca acgaactgca cgaagtgttc ggaataacca ttcgccacat caatacaaac
    36061 gaagtttaat gccgggttca ggtcgagaat ctgtttagtt ttttcgaaat ccgcatcaga
    36121 cgtgccggta gaaaccatca catgtttcag cacatcagcg gaagaattgt tgataaacgc
    36181 ttgccactct tcgacagaaa agtgtttatg cacagcagtc agaatatcaa aagaagccag
    36241 cgcagaggcc atagaaaatg tgcctacggt gtccatattt gcggcgataa tcggcacgcc
    36301 ggaccagctc tgacctgaat gtttgaaggt gaattgacgt tccagttcaa catcggaacg
    36361 gcttttaaga gtggagcgtt tagggcggat gagaacgtct ttaaaaccta acttcagatc
    36421 ttcttcaata cgcatgtgcg gattcctggg gttaatggct ataagggtaa gagcgcaact
    36481 ccagtgacgc tatcatacgc actaatcaat gcggcgcaag actgcgaaat tgtctttttt
    36541 tacgctacaa tcccataaat ttacctggta aatagtgagc gaacgtttca tcaatttcac
    36601 tttcgcgcgc tcatttttaa cgttttgata atcatgatga attcccggga atataaatta
    36661 tggggtatat agttgcctta acgggaggca ttggcagtgg taagagtacc gttgccaatg
    36721 cgtttgctga tctcggaatt aacgtcattg atgccgatat tattgcgcgt caggtggttg
    36781 aaccaggtgc acctgcgcta catgccattg ctgatcactt tggcgaaaac atgattgctg
    36841 ctgatggaac attgcagcgc cgggccttgc gcgagcggat cttcgccaac ccggaagaga
    36901 aaaactggct taacgacctg ctgcatccgc tgattcagca agagacgcaa caccagatcc
    36961 agcaagcaac ttccccctat gtactgtggg ttgtgccatt gctggtagaa aactcactgt
    37021 ataaaaaagc gaatcgagtg ctggtggtgg atgtcagccc agaaacgcaa cttaagcgca
    37081 ccatgcagcg cgatgatgta actcgcgaac atgtcgaaca aatccttgct gctcaggcaa
    37141 cgcgcgaagc ccgccttgcc gtggcagatg acgtcattga taataacggc gcaccggatg
    37201 ctatcgcatc ggatgttgcc cgcctgcacg cacactattt gcagcttgcg tcgcagtttg
    37261 tctcacagga aaaaccgtaa tgcagaccca ggtccttttt gaacatccac taaatgaaaa
    37321 aatgcgtaca tggctgcgca ttgagttttt gattcagcaa ctcaccgtta atttacccat
    37381 cgttgaccac gctggcgcgc tgcatttctt ccgtaatgtc agtgaattac tggatgtttt
    37441 cgagcgcggc gaagtccgca ctgagctgtt gaaagaactt aaccggcagc aacgtaaact
    37501 ccagacctgg attggcgtgc ctggcgtgga ccagagccgt attgaagcat taattcagca
    37561 gttaaaagcg gcggggagcg tattaatttc cgcgccgcgt atcgggcaat ttctgcgtga
    37621 agatcgtttg attgctctgg tgcgtcagcg actgagcatc ccaggcggct gttgcagctt
    37681 tgatttacct acattgcaca tttggctgca tctaccccag gcgcagcgcg acagccaggt
    37741 agaaacctgg attgccagcc tgaacccgct cacccaggca cttaccatgg tgctggattt
    37801 aattcgccag tcggccccct tccgtaaaca aaccagcctg aatggttttt atcaggataa
    37861 cggtggcgat gccgacttgc tgcgcctgaa tctgtcgctc gattcacagc tttatccgca
    37921 aatttccgga cataagagcc gttttgccat tcgttttatg ccgctggaca ctgaaaacgg
    37981 acaggtaccg gaacgtctgg atttcgaact ggcctgttgc taaggagtaa aaatgtcaga
    38041 aactattacg gtgaattgcc caacctgcgg gaaaacggtg gtgtggggtg aaatcagccc
    38101 gtttcggcca ttttgctcca aacgttgtca gctgatcgac ctcggagaat gggctgctga
    38161 agaaaaacga atccccagca gtggcgatct ttccgaaagc gatgactgga gcgaagagcc
    38221 aaagcagtga catttgtctg atgcctcacg taggcctgat aagacgcgga cagcgtcgca
    38281 tcaggcatct tgtgcaaatg tcggatgcgg cgtgaacgcc ttatccgacc tacagacgct
    38341 taagcttcgc aattaccggt tcattggctg gcggaaaatc atcggcatta agaccgacca
    38401 gcgacatcca ctcaccgggt tgcccctctt taccccacgg caccccttcc caactttcga
    38461 ccagccagaa ccacagagtt atatgcctgt ccgggaattc atattccagt ttttcaaata
    38521 gcgaaaaatg ttgtggggta atcccgacct cttcctgaag ttcacgcacc accgcctgtt
    38581 ccggcgtttc gcccatttca attttaccgc cgggaaactc cagtttattc gccatgtgcg
    38641 catctgctgc gcgacgtgtt ataaagattt cattgttctc gttgcgaata atacctaccg
    38701 caatttgcag ctttttcatt gtcttaaacc tataaaaaag gcgcagaatc ctgcgccttt
    38761 tacttcaact gttagctttt attgcaggcg accatggcac tgcttgtatt ttttaccaga
    38821 accgcacggg caaggatcgt tacgtcctac tttgcgttca ccggtttgcg ccgccagtgc
    38881 agctgcggct gcagagtcgt catcctgatg gctaagctgc tgcatttgtg ctaaacgctc
    38941 ggcttccata cgacgctgtt gttccagctc ctcaacctct tcaggcatac gtacctgaac
    39001 tttgctcagc gtactgataa cttcatattt caacgactcc agcatcgctg caaacatgga
    39061 gaacgattca cgtttgtatt cctgcttcgg atctttctgt gcatagccac gcaggtggat
    39121 accctgacgc agatagtcca tcgctgccag gtgctctttc cacagggagt caagcgtttg
    39181 cagcatgacg cctttctcga agtgacgcat catctcagca ccaaccactt cttctttacg
    39241 ctgatacact tcgatggact gcgccagaat gcgctcacgc agcgtctctt catgcagttc
    39301 tggttcttta tccagccact cggcaattgg taaatcgagg tcgaaatcgt tcttcagacg
    39361 ttcctgcagc cccggaatat cccacatttc ttccagcgac tgtggcggaa tgtaggcatc
    39421 aatggtggct ttgaacacat cttcacgaat gctgttgatg gtttcgctca catcgctgac
    39481 atccagcagt tcgttacgct gggagtaaat ggcgcgacgc tgatcgttag ccacgtcatc
    39541 atattccagc agttgcttac gaatgtcgaa gttacgactt tcaactttac gctgggcgtt
    39601 ggcaatcgct ttagtcaccc acgggtgttc aatggcttcg cctggcttca tacccagttt
    39661 acgcatcatg ccggatactc ggtcggaagc aaaaatacgc atcagcgcat cttccatcga
    39721 caggtagaaa cgggaagaac cagcatcccc ctgacgacca gaacgaccgc gcaactggtt
    39781 atcgatacga cgggattcgt gacgctcggt accgatgata tgcaggccac ctgcttccag
    39841 taccgcatcg tgacgtacct gccagtcggc tttaattttt tcaatttgct ctgcggtcgg
    39901 attttccagc gcggcaactt ctgcctgcca gctacctccg agcacaatat ctgtaccacg
    39961 acccgccatg ttggtcgcga tagtcaccgc agccggataa cctgcctgag caacaatcgc
    40021 cgcttcgttg gcgtggaatt tggcgttcag gacattgtgc ttaataccgg ctttggtcag
    40081 ttcgtttgac accagctccg atttttcgat ggagatagta cccaccagca ccggctggcc
    40141 tttcgcagta cgttctttga tatcttcaat gatcgcctga attttttccg cttcagtcat
    40201 gtagactagg tccggcagat ctttacgaat cattggacgg ttggtcggaa caacgacggt
    40261 atccagtttg tagatggagc taaattcgaa agcttcggta tcagcagtac cggtcatccc
    40321 cgccagtttt tcatacagac ggaagtagtt ctggaaggtg atcgaagcca gcgtttggtt
    40381 ttcgttctgg atctgcacac cttctttcgc ttccacagcc tggtgcagac catcggacca
    40441 gcgtcggccc tgcatggtac gaccggtgtg ttcgtcaacg atgataactt caccatcttt
    40501 aacgatgtag tcgacgtcac gggtaaacag cgcatgagcg cgcagcgccg ccgttacgtg
    40561 gtgcatcagc atgatgttgg ccggagagta cagagactcc ccttcatcca tgatgccctc
    40621 tttcaccagc agttcttcaa tcagcaccag accacgttcg gtcaggttca cctggcgaga
    40681 tttttcgtcc accgagaagt ggccttcgcc ctggaaggtt tcggagtctt ctttttcctg
    40741 acggatcagg tgcggaataa ttttattcgc gcgtttatac atttccgagc tgtcttctgc
    40801 cgggccggaa atgatcagcg gtgtacgcgc ttcatcgatc aggatggagt ccacttcgtc
    40861 caccagcgca tagtgcagtt tacgctgtac acgttcttca gggctgaacg ccatgttgtc
    40921 gcgcaggtag tcaaagccgt attcgttgtt cgtaccgtaa gtgatgtcag ctgcgtaagc
    40981 ttcgcgcttt gccggtgctg gcatgcccgg caggttgata ccaacagtca ggccaaggaa
    41041 ttcaaacagc ggacggttgt tttcggcgtc acgttgcgcc aggtagtcgt tgacggtaac
    41101 tacgtgcacg cctttaccgg ttagtgcgtt caggtaagca ggcagcgttg cggtcagggt
    41161 tttaccttca ccggtacgca tttcggcgat gcagcgttcg ttaagaacca taccgccgag
    41221 taactgaacg tcgaagtgac gcataccaaa gacgcgctta cttgcctcac gcaccacggc
    41281 gaaagcttcc gggatcagat tttccagcac ttcgcctttt tccagacgcg cacgaaactc
    41341 tgcggttttc cctttcagtt cttcatcgga gagtttttcc atctccggtt ccatggcatt
    41401 gatgatgttg accactttgc gcatccggcg cagggtgcga tcgttacgac taccgaaaac
    41461 tttagttaac aatttgatta gcataataaa atctcaaacg ccccgcgttg cggagttaat
    41521 aaaatgaagt aaaggtttat tgttgttagg tgaggcgttg agggccagca cggatgcctt
    41581 gcgcctggct tatccagacg ggcgtgctga attttgcttg tggggtaaaa tgcgcataat
    41641 caatgcgata acccttttca gacggcgtgc cttcctgggt cagcagcgcg ctgagcgtat
    41701 ccagtaatgc aagatgttgc gcctgaagag gcaaagattc ttcagcaacg ggcagtgttt
    41761 gcggtgccat tgcgaaagaa agatgacgga ttaccgtgcg aatagcatgt tgatgccagt
    41821 aatcaacgga atagttcgaa ttcgggcggc gcgtgttcgc ttccagcaag gccaattgac
    41881 caaagttaac tttggctgaa ggctcgtggt tgcgggttgt cgcttttgcg ggcgcgtttg
    41941 gttcggcggc gttgctgagc gcaggcaaac ctaaactcgc cgcgaccatc cctaataaga
    42001 gatgcggcca gaagtagcgt ttaccaaact gtcgccagcg cgtcagtatt ccactcacgt
    42061 tattgccatc ccgatcgaat tcaaacgtat ttagcgcaca aatcttatca ttaaagcctg
    42121 aagtccacag cacgaatgaa gataagttgt gcgtaaaagt gcttaagaaa acagcgttcg
    42181 caccgttttt tatagctctt ataaaattca aaaaaaaacg actggttcac ctagccggag
    42241 agagtgccag atttgccagt cgaattttat acgacagtat aaatgtcgtt atgccagtac
    42301 agctgaaggc gctttgaagg ccaacggcag ttctgcgtcg tcctggaagg tcacatattc
    42361 ccaggcttcc tgtttcgcca ggacagcctg cagcagtttg ttattcagtg catgaccgga
    42421 tttataagcg gtaaatgcac caataatatt gtgaccacac atgaacaagt caccgatcgc
    42481 atcgagcatt ttgtgacgca caaattcgtc ttcaaaacgc aggccgtctt cgttcagtac
    42541 gcgataatcg tcaacaacga tggcacaatc gaagctgccg cccaggcaca aaccacggga
    42601 ctgcagatat tcgatatcac gcatgaaacc gaacgtacgc gcacggctga tctggcgcat
    42661 aaacgcatca gcggagaagt tcatcgcata gcgctggttg ctggaatcaa tagccggatg
    42721 gttaaaatcg atggtgaaat ccagcgaaaa accattgtac ggcttaaatt cagcccactt
    42781 atcgccatct tcgacacgaa cagtctcttt gatgcgaaca aattttttgg cgcagttcaa
    42841 ctcgtcgata ccggcgtcaa gcagcaggta tacaaacgga gcggcgctgc cgtccatgat
    42901 cgggatttcc ggcgcgttaa cttcgataac aatgttatcg atgcccaggc ccgcgagagc
    42961 agcattgagg tgctctacgg ttgaaatccg tacatcatgc tcgttgacca gacacgtaca
    43021 gagcatggta tcacgcacag atttggcatc ggccgggaaa tctaccggtg gattcaagtc
    43081 ggtgcgacga tagatgaccc cggtgttggc cggcgcaggg cgtaacgtca gggtgacttt
    43141 cttgccggta tgtaaaccga cacccgtcgc ctgaacgata cgtttaagtg tcctttgttt
    43201 gatcatcgta ttatctcgcc aaattaccta tccaaccgaa gtgtactata cattcggcgg
    43261 gccagtttag cacaaagagc ctcgaaaccc aaattccagt caattcttaa tcagcttgct
    43321 tacgtaggaa tgctgggata tccagataat ccggctcttt cgcagtttgc ggcgcattgt
    43381 cattcactac tttagcaacc ggcttctgct cctgggtcag cggagccatc ccatgctgct
    43441 ggtagcgatc catcactggc tgctgaacct gcttattggt caccagagtg atttcaggac
    43501 gtttgtccat gccgatacct gtcgcaacaa cggttacgcg cagctcgtca ttcatatccg
    43561 ggtcaagaga agtaccgata accacagtcg cgttgtcgga agcaaatgca cggatggtgt
    43621 tacctaccgt ttcgaactca tccagacgca ggtcgaagcc cgccgtgatg ttaaccagca
    43681 cgccgcgcgc gccagacagg tcgatatctt ccagcagcgg agaagagata gccatttcag
    43741 cagcttcttc cgcacggtct tcaccgctcg ccacgccaga acccatcatt gcgtagccca
    43801 tttcagacat tacggtgcgt acgtctgcaa agtccacgtt catcaaaccc ggacgagtaa
    43861 tcagttcggc gataccttgc acagcgcctt tcagtacatc gttcgctgcg ccaaacgcat
    43921 ccagcaggga gataccgcgg cccagaactt tcagcagttt gtcgttcggg atagtgatca
    43981 gagagtccac atgcttggac agttcagtga tcccctgctc ggcgaatgcc atacgcttct
    44041 tgccttcaaa gttgaaaggc ttagtgacga cagcaacggt caggataccc aaatcttttg
    44101 ccacttcagc gacgactggt gctgcacctg taccggtacc accacccata cccgcagcaa
    44161 taaagaccat gtctgcacct tccagcgccg cacgcaatgc atcgcgatcc tcatcagccg
    44221 cattgcggcc aacttctgga ttagcgccag cgcccagtcc tttggtgata ccgctaccga
    44281 tttgaatcgt ctgtccaacc gctgttttac gcagcgcttg tgcatcggta tttaccgcga
    44341 agaattcaac accttcaata cgctcgcgca ccatgtgttc aacagcatta ccgccgccgc
    44401 cgccgacgcc gatgacttta atcaccgcgt cattggtaag ttccattggt tcaaacatag
    44461 tttctctccg atttgtgcct gtcgcctgag gccgtaatca tcgtcggcct cataaaaatt
    44521 aaaactcttt tcgcagccaa ctattgagtc gcttgatcca cgagccaact gatgctgtaa
    44581 cacgtttttc tacttcagct tcaccgttaa gatgtgactc tttcccatag tgaagcaatc
    44641 ccaccgccgt cgaataatac ggctcctgag cataatccgt taaaccggta atgttcagcg
    44701 gcgcgccgat acgcacttgc gtatgaaaca cgcgctgagc acaggctgca agaccttcga
    44761 tctgcgctgc gccaccggtt aatacaatgc ctgccgccag gtgatgttta accccttgtt
    44821 ggcgaagctt ttcctgcaac tgcaatatct cttcgttgac caggttgagc aactcggtat
    44881 agcgcggctc gatcacctct gccagggtct gacgttgcag actccgcggc ggacgaccgc
    44941 ctacgctcgg cacttccacg ctctcatctt ttccaacgat ggaacccagc gcacaaccgt
    45001 ggcgaacttt aatcgcttcg gcgtcgcttg gcggcgtgcc aaaggcgtaa gcgatatcac
    45061 tggtcacgac attgccagca taaggaatta ccttagtgtg gcgcaatgcc ccaccggtat
    45121 aaacggcgat atccattgta ccaccaccga tatcgacgac gcagacaccc agttcacgtt
    45181 catcttccgt caataccgaa taacttgatg ccagtccggc aaatatcagt tggtcaactt
    45241 tcagcccaca acgttcaacc gctttgacga tgtttttcgc catatcgttg tgacatgtga
    45301 tcaggtgcac ttttgcctgc atccgcacac ccgaaagtcc taccggattc ttgatccctt
    45361 cctgatagtc aatcgcatac tcttgcggga tcacatgcag cacacgatgc tcatcgcgca
    45421 cacgcaccga tttcgcggta tggacgacat tttccacatc ttcttgcgtc acttcttctt
    45481 cagaaatagg caccatacca atttcattct ggcagctgat gtgcttacca gaaagcgcca
    45541 gatataccga agagatctga caatctgcca tcaattctgc ctggtcaatg gcgcgttgta
    45601 cgcacttgac cacggattcg aggtcgttca ccccgccttt atccatacca cgtgacgggc
    45661 agctgcccac gccaatgata ttgaccatac cgtcgggcag aacttcccct actaaagcgg
    45721 caaccttcgc ggtaccaatt tccagtccta ctaccagttt tctgtccgtc gccttgatca
    45781 ttgttgttct gcctgtgcct gattttgttg ctgagtagat tcctctggcg gcaagggcgc
    45841 ccagcctact gccgctccag agtcataacg caaatcaacg tagctaatcc gtttgccatc
    45901 ggtttgcgcc tgctgctgta aaaccggata aagttctaca aagcgagcca aacgtttcat
    45961 cgtatcgccc cggccaagat tgagcttaat atcgttattc agcgtcaact gccaggaacg
    46021 ccgcgcggtc atcgccgctt ccttcagagt aaatctgtcc tttgccagca tctgccccat
    46081 ttcgcgatag ccctgcaaca cttcattagc gctgccttcc gggccataca gcattggaag
    46141 cacctgcttg ctggtgcgat ctggcggcac gctgaaggtg tttccttccg cgtctaccat
    46201 gtgttgatca ttccaccgcg caatcggcac atattcaacc agatgaatct tcaattcatc
    46261 aggccactgc tttctgacgc tcacctgctt aatccacggc aggcgttgtt ctatttgcgt
    46321 ctggatgatg ttgacatcct gggtcataaa ggtacccggc tcacccaatg ccaggatcga
    46381 ctgccggata tcgtcattac gcgtgtaatg gcgttcaccg gtcaacacca gctttgagag
    46441 cggcaggcgt tgcgcatctt ccatccagct caacacgacc cagccgctca ccaacactgt
    46501 cgttaaaacg gtcagcagga aaaggatccc cgccagacgc gttccattat tgcggcgaga
    46561 agaaacctct tcttcgctgt ttcgcgtgtt cagagcagcc tgcgacatat tagtccgcca
    46621 gttccagaat tcgtactacc aactgcgaga agctcatacc tgcctgacgt gccgccatcg
    46681 gcaccaggct gtggctggtc atacccggtg aggtattggc ttccagcaaa taaaactgtc
    46741 catcgctgtc cagcataacg tcaatacgtc cccatccttt gcaacctaac gtcgtccatg
    46801 ctttcagcac taatgcctgc aaattggcct cttgtgacgc ttccagacct gcggggcaga
    46861 aatactgtgt ctcatcagag agatacttcg cctcataatc atagaaggtt ccggacggtt
    46921 gaatacgtat tgacggtaaa atttcttcac cgagtatcgc aaccgtgaac tccggcccac
    46981 ttagccattt ttcaatcaat acttcttcat cgtgctgaaa tgccaatctt aatgcatctt
    47041 gtagagcatt ttctgctact acttttgaca ttcccacact ggaaccttcg cggctcggct
    47101 taacgataac cggcaaaccc agagcagaaa tttctgctaa ctgcttatcg ctcaggcctt
    47161 tttcaaactc tgcgcgggtt aatgctaccc acggcgcgac cggtaaaccg gcaccttgcc
    47221 acagaagttt gctgcgtagt ttatctattg aaagcgcaga tgccatcact ccgctgccgg
    47281 tataaggcaa gcccatcagc tcgagcatgc cctgcagcgt accatcttca ccgccgcgac
    47341 cgtgtagcgc gataaacact ttctgaaaac ccatcgactt cagttgcgtc acgtcgactt
    47401 ctttcgggtc gacaggatac gcatcaatac cgccttcacg cagcccggct aacaccgctg
    47461 cgccagaatt cagagaaact tcccgctcag cggaagtccc gcccaacagg accgcgattt
    47521 tatcagtcat gttgttcttc ctccggagtt tgcggcttca gtttgatttc agctaaagaa
    47581 cgggcaattt ttccgatatt accagccccc tgaacgagaa tcaggtcgtt accggttaat
    47641 accggtgcca gcatctcggc tacctgcgca gggtccggca ccagaatggg atcaattttc
    47701 ccacgtccac gaattgtgcg acacagcgaa cggctgtccg ctcccggaat tggcgcttcg
    47761 ccagccggat acacttccag catcaacagg gtatcaacct gcgtcagcac attggcgaaa
    47821 tcatcataca ggtcgcgcgt acgggtaaaa cggtgcggct gaaacagcat taccaggttt
    47881 ttatccggcc agcctgcgcg cgccgcttta atggtggcgt caacttccgt cgggtggtgg
    47941 ccgtagtcat cgaccagcat tgccgtaccg cttttaccat tcactggctc cagcgggaat
    48001 tcaccgagga aatcaaaacg gcgaccagtc ccctggaagc tctccagcgc acgcaaaata
    48061 gcctcgtcgt caattccctc ttccgtagca accgcaaccg cagctgcggc gttcagcgcg
    48121 ttatgacggc ctggcgcatt cagggtgacg cgcatcggct ctttgtcctg acgcagcagc
    48181 gtaaagtgcc cctgcgggcc aatctgctga taatcttcta cacgcacgtc ggcatcttcg
    48241 ctgaagccgt aagttgtggt ctgacgtccc acacgcggta acaattcgcg gatcaccgga
    48301 tcatcaacac acatcaccgc acgaccgtaa aaaggcaggt tgtgcagaaa attaataaag
    48361 gtctgtttta aattctcaaa gtcgccctgg taggtatcca tgtggtcggc ttcgatattg
    48421 gtgacaatcg ccaccatcgg ttgcagatgc aggaacgatg catcactctc atctgcttcg
    48481 gcaatcaggt accgaccatg ccccaaacgc gcatgaaccc ccgccgcttt taccagcccg
    48541 ccgttaacga aggttgggtc gagccccgct tctgcgtaga tgctggaaac catcgcggta
    48601 gttgtcgttt tgccgtgcgt tcctgcaatg gcgatgccat gacgaaaacg cattaactca
    48661 gccagcattt cggcacgacg gatcaccgga atacgcgctt catgagctgc gacaatttcc
    48721 gggttgtcgg cagaaatggc gctggaaaca acgaccacgc tggcatcacg tacgttttcc
    48781 gggcgatggt tgaaataaat cgtcgcaccc agattcagta actgctgcgt gaccggattt
    48841 ggcgctaaat cggaaccact gatctgataa ccttcattgg ccagaacttc ggcaataccg
    48901 cccataccgg caccaccaat gccgacaaaa tgtatgtgcc gaacgcgacg catttcgggc
    48961 acgatggaac gcagttttgc caattgttgt gtattcattc tttacgccat taacttctta
    49021 aattcatacg atgcaaaagg catcgctaca attacgcccg ggcagcccgg ctcacttcat
    49081 ttgccactcg ctcggtggca tccggaatgg atgcagcgcg ggcgcgttct gccatggtta
    49141 ataaggtttc tcgcgaccac ccggccaggg tgttggcgac agcatccacg ctaagctgtg
    49201 gctgctcgat aattttggct gcgcccgctt tttccagcgg tagcgcattc cagtattgct
    49261 ggcggtcttt atgttgaaac ggcacaaaca acgccggtag tcctgccgcg gcgatttcac
    49321 tcacagttaa agcaccggag cggcaaacga cgacatccgc ccacgcatac gccgccgcca
    49381 tatcatcaat aaattcagtc actttatgct gcggttgccc cgcttcggca tacgcctgtt
    49441 caacggattg ttgcgaacct ttgccgctct gatgccagat agtgactgaa tcacccagtt
    49501 tcgcagcaac ctgcggcatt gtctggttaa aaatgcgtgc gccctgggaa ccacccacta
    49561 ccaacacacg aaccggacct tcacgtccag ccaaacgttg ctgcggcaac ggcagcgcca
    49621 gcacatcggt acgcaccggg ttacccacca cttccgcatt agggaaagcg cctggaaacg
    49681 cctgcatcac tttggtagca atcttcgcca gccatttatt ggttaagccc gcaataccgt
    49741 tttgttcatg aagtacaacc ggaatgccta acgaccacgc ggccagacca cctggacctg
    49801 atacatagcc gcccataccg agtaccacgt caggtttgta cgctttcata atcgcccgcg
    49861 cctgacgcca ggcgttgaag atacgcagcg gcgcagctat cagtgctttt ataccttttc
    49921 cacgcaaacc agagatacga atgaaatcaa tttcgatgcc atgttttggc actaagtccg
    49981 cttccatacg gtcggcagtc cccagccagc gaacttgcca accctgagcc attagatagt
    50041 gcgcaaccgc cagtcccggg aatacatgtc caccggttcc gcctgccatc accattaatc
    50101 gctttccttg accactcatc gtgaacctcg tacaaacgcc tgcgctttct ccagacgcgt
    50161 ttcataatca atacgcaaca gcatcatgat ggctgtcgac ataatcagta agctcgaacc
    50221 accgtaactg attagcggca atgtcagacc tttggtcggt aacatccccg ccgccgcgcc
    50281 tacgttaacc agcgcctgga agctaaacca gatgccaata gaacaggcga gaaaaccgga
    50341 aaaacggtgg tcaatttcta atgctttgcg gccaatcgac atcgcacgaa aagcgacgaa
    50401 gaataccatt aaaagtgcta gtaccacacc gacataacct aattcttcgc cgataatggc
    50461 gaaaataaag tcagtgtgcg cttccggcag atactccagt ttttgtaccg aattacctaa
    50521 accttgcccc caaagttcgc cgcgaccaaa cgccatcagc gattgcgtta actgatagcc
    50581 gctgccaaag ggatcttccc acgggttcca gaatgcggta acacggcgga tacggtacgg
    50641 ttcggcgagg atcagcaaca caaccgctga aatgcccata ccgataatgg caatgaactg
    50701 ccacaatttc gctcccgcca ggaacaacat cgccagcgta gtcacaaaca acaccaccac
    50761 cgtaccaagg tctggctgtg ccagcagtaa cactgccaac accagaatca cgcccatcgg
    50821 tttcaggaag ccgcgcaggt tattacgtac ttcgtcgcct ttacgcacca gatagttggc
    50881 gatatagcaa aacagcgaca gttttgtcag ctccgcaggc tggatacgca gcaaaccgag
    50941 atcgatccaa cgcgatgccc ctttaacagc gctacccact accagcacga tcatcagcag
    51001 gatgatagat ccgagcagca tcgtggcact gtagcgttgc cagaactcca tcggcagacg
    51061 cagcgtaatg atcgccagaa taaacgccaa aatcagatag acaccatcac gcttcgcgaa
    51121 gaagaacgga tcgttggtta agcgttgccc tatgggcatt gacgccgagg tcaccatgat
    51181 aaagccaatc gccgcgaggc cgaaggtcag ccacagtaag gtgcgatcgt acatgatcag
    51241 gctgtcggta tctttttccc gcgagcccat cacccagccc tttagcgccg tggagatcca
    51301 gaccaggata ctgaatcctg gcaggcgcgg cattttcagg cgagggagag ataaacgcat
    51361 caacctaact ccttcgccag acaggcaaac tcattgcctc gttgttcaaa gttcttgaac
    51421 tgatcaaggc tggcacaggc cggggagagc agaaccatat cgcccggctg aacacgcgga
    51481 gccagcaagc gcatagcctg ttccatagtt tcggtttgtt ctgccacttc cgggcgtagc
    51541 gccgccagct gcgcgccgtc acgaccgaaa caatacagac gtacgttatc gccattcagg
    51601 taacgcgcca gtgggctaaa gtccgccgat ttaccatcgc cgcccagcaa caaatgcagc
    51661 gtgccgtcta cgtgcaggcc attcagcgcc gcttccgtac tgccgacgtt ggtcgctttt
    51721 gaatcgttaa tccagcgcac accgttatgc tccagcacca cttcaaagcg atgcggcaga
    51781 ccggtgaagg tggttaacgc tttcaggctg ctggcgcgcg gtaaccctgc ggcatctgcc
    51841 agcgccagcg ccgccagcgc attggtgtag ttatgctgcc cggaaagttt catctctttc
    51901 acattcagca ctttctcgcc tttaacccgc agccaggttt cgccctgctg atgattcagg
    51961 tgatagtcac ccatgttgac gccaaagctg acgcagcgtt catccgcacc gcgaatcggc
    52021 attgttaagg catcatcagc attaaccacg caaactttcg cgttttcgta aatgcgcagt
    52081 tttgctgcac gatactgttg taaaccaaac gggtagcgat ccatatgatc ttcagtcacg
    52141 ttcagaatgg tcgctgctac cgcctgtaag ctggaggtgg tttccagctg gaagctcgac
    52201 agttccagca cgtacagttc acactcatca tccagtagca tcaacgcagg caggccaata
    52261 ttgccaccca caccaacgtt aacccccgcc gctttcgcca tttcacccac tagcgtggtg
    52321 accgtgcttt tgccgttaga accggtaatc gccacaatcg gtgcttgtgc ttcgcgacaa
    52381 aacagctcga tatcgccaac gatttcgatt ccggcatcag cggcagcgct taaggatgga
    52441 tgcgccagtg caataccggg actggcgaca atcagatctg ccgccatcag ccattcatca
    52501 ttcagactgc ccgtgtggcg ttctacggct tcgggtaatt tatccaggcc aggcggtgtc
    52561 atacgcgtat ccataacgcg cggcgtcaca ccgcgagcga ggaaaaagtc cacgcaggaa
    52621 agcccggtga ggcccaggcc gataatgaca acatttttac cctggtaatc agccatgatt
    52681 aacgtacctt cagcgttgcc agaccaatca ggaccagcat cagcgaaata atccagaaac
    52741 gcacaatgac gcgcggttcc ggccagcctt tcagttcata gtggtgatga atcggtgcca
    52801 tgcggaaaat acgttgtccg cgcagtttaa aggagccgac ctgcaggatg acagaaagcg
    52861 tttctaccac gaacacgccc cccataatca ccagcaggaa ttcctgacgt agcagtacgg
    52921 cgataatgcc taacgcacca cctaacgcca gcgaaccgac atcgcccata aagacctgcg
    52981 ccggataggt gttaaaccac aggaagccca gtcctgcccc gactatcgcg gtacagacaa
    53041 taaccagttc cccggcgtgt cgcagatacg gtatatgtaa atagctggca aagttcatgt
    53101 tgccggtcgc ccacgccacc agcgcaaaac caccggcgac aaatacggtc ggcataattg
    53161 ccagaccgtc aagaccatcg gtcaggttta ccgcgttgcc agtacccaca atgacgaagt
    53221 aagccagcag aatgtagaac agccccagtt gcggcatcac atctttaaag aatgggacca
    53281 ccagctgcgt tgcgggcgtg tctttgccgg caaggtacaa agcgaaagca acacccagcg
    53341 caatgaccga catccagaaa tatttccaac gagcgatcaa ccctttggtg tctttacgca
    53401 ccactttgcg ataatcatca acaaagccaa taacaccgta acctaccagc accaccaaca
    53461 cgcaccagac gtacggattg gacgggtaag cccacagcag cacggagatc acaatcgctg
    53521 tcaggatcat aatcccgccc atggtcggcg taccgcgctt gctgaagtgt gattcaggac
    53581 cgtcgttacg cactacctga ccaaaggaaa gtttttgcaa atgagcaatc atgcgcgggc
    53641 ccatccacaa tgagatgaac agcgcggtca gcaggctgac gatggcacga aacgtcagat
    53701 aggaaaagac gttaaagccg gaataatatt tgaccaaatg ttcggccagc caaactaaca
    53761 tgtcccattc tcctgtaaag cgcgtactac ctcttccatg gcagcactac gtgaaccctt
    53821 aactaaaatc gtaattacct gttgctcagc aatcagtgat ttaagacgcg taattaacgc
    53881 agttttatca gcaaaatgtt cgccaacgcc gctggcggtg ctgatagcat ggctttgttt
    53941 acccatgctt aacacgcggt caataccagc agcttttgcc gcctcgccca cctgtacatg
    54001 gcaggcttcg ctttcagcgc ccagttccgc catatcgccc accaccagca cgcggtagcc
    54061 cggcatttca gccagtacct gaacagctgc ggtcattgaa ccgacattgg cgttgtagga
    54121 gtcgtcgagc agcaactggt tttctgccag ttggatgggg aacagacggc caggaacagc
    54181 tttcagattt gccagccccg ctttgatagc atcaagcgtt gcgcccacgg acatggagag
    54241 cgcagcggct gccagcgcat tcgcaatatt gtgacgcccc ggcaacggca gcagaacatc
    54301 gacgctaccg gttggggttt gtagggtaaa ttccataccg tgcgaggtca catggatatt
    54361 ggtggcggtg aaatcgctgt tggcggcatt gggtgagaaa cgccacactt tgcgtgagcc
    54421 aattacgctc tgccagttca gccagtcgtt gttgtcggcg ttcataatgg cgataccgtt
    54481 ttccggcagg ccgctaaaga tttcaccttt cgctttcgcg acacccgcaa gcgagccaaa
    54541 accttccaga tgcgccgctg ccaggttgtt gaccagcgcc gcttccgggc gagtcagact
    54601 cacagtccag gcaatctcgc cctgatggtt cgcgccaagt tcaataactg cgtaatcgta
    54661 ttccggcgtt aagcgcaaca gcgtcatcgg tacaccgatg tcgttgttga gattgcctgc
    54721 cgtataaagc gtgttgccgc actggcttaa aatcgccgcc gtcatctctt taacggaggt
    54781 tttgccggag gaccccgtca gagcaaccac gcgcgccgga acttgctggc gaacccatgc
    54841 agccagttca ccaaacgcca gacgcgtatc cttgacgatt aactgcggca ggtcgatgtc
    54901 cagcggacgg ctaaccagta gtgcgcctgc gccgccagct ttcgcctggt cggcaaaatc
    54961 gtgggcatca aaacgttcac ctttcagggc aacaaacagg cagcccggcg tcagttttcg
    55021 ggtatcagtg gttacagcat caagggtgat atctgcacct tgcagttcac cgttgagaat
    55081 atcggtaagt tggctaaggg ttacgctaat catgcaatca cccccagcag acgcgccacc
    55141 gtgacgcgat cggagtagtc cagacgctga ttgccaacaa tctggtaatc ctcatggcct
    55201 ttgcccgcga ccagtaccac atcattctct ttagcctgca taacggcgca agtcaccgct
    55261 tcagcacggc cttccatcac tttggcatgt ccggcatcta acattcccgc cagaatatcg
    55321 ttgatgatgg cacgcggttc ttcggtacgc gggttatcgt ccgtcaccac cgccacgtca
    55381 gcaaactctt cggcaattgc gcccatcagt ggacgcttac ctttatcgcg atcgccacca
    55441 cagccaaaga cgcaccacag cttgcccgca cagtgcaggc gcgccgcctg taaggctttt
    55501 tccagtgcat ccggcgtatg cgcgtaatcc accaccaccg tcggtttacc tggcgcagtg
    55561 aacacttcca tacgtccgca aaccggttgc agacgcgcgg cggttttcag cagatcagcc
    55621 agtggatagc ccagcgccag cagtgttgcc agcgcgagca acaggttgct gacgttaaaa
    55681 gcgcccatca gatggctttc aatttcgcca tcgccccagc ttgaactaaa gcgaatggtc
    55741 gcgccgctgt cgtgatagtt cacttcggtc gctttcaacc agcgtccgtg acagttcgga
    55801 ttgatatgat cttccattga taccgcaacc gcgtccggca gttttgccag ccagcggcgg
    55861 cccacttcat cgtcggcgtt aataatcgcc tgaccgcaat gatgttcaga ataaagcagc
    55921 catttcgcag cttcgtagtg ttccatatca ccatgataat caaggtgatc gcggcttaag
    55981 ttggtaaaga ccgacgccgc aaatttcaat gccgccacac ggtgctgtac cagcccgtgg
    56041 gaggaaactt ccattgcgca aaaagtcgcg ccctgatcca ccagccccgc cagctcatgc
    56101 tgaacatcga ctgccgaacc ggtagtattt tctgtcggga tcactttccc cagcagaccg
    56161 ttaccaacgg tgcccattac cgcgctggtt tcgccaagca gttggctcca ctgcgccaga
    56221 agctgggtcg tcgtggtttt gccgttggtg cccgttacgc ctacgagacg taaattgtca
    56281 gagggttcat ggtaaaagcg gcccgccagt gcagataaac gctcgttgag ctggctgaga
    56341 tagatgaccg gtacgccgtg catttcacgg atttcaccgt cggtcgcctc atctttcgcc
    56401 tctgcaataa tggcagccac accttgcgct atcgcctgcg ggatatatcg acgcccgtcc
    56461 gcctgatgac ctactacagc tacaaagaga tcgcccgccg cagccacacg gctgtcgagt
    56521 gtcatctctc gcagtgctcg cgaaggtgcg tctggcaccc acggagcaag aaggtcgcgc
    56581 aaattacgat ctgccacctg tcccctcgcc ttgattaatc acaaattcat ttttatcgcc
    56641 cgttgtcagc gcatccggct cgatgttcat ggtacgcaat acgccgccca tgatggtacc
    56701 aaagaccggc gcggaaacgg cgccgccgta gtatttaccc gcctgcggat cgttgataac
    56761 aacaaccagc gcgaagcgcg gctgactcgc aggcgcaacg cctgcggtat aagcaatata
    56821 tttattgatg tagcgaccgt ccggcccgac ctttttcgcg gtaccggttt taatggcgat
    56881 acgatagcct ttaatcgccg ccttcacgcc gccgccgcct ggtagcgcca cgctttccat
    56941 catatgcacc acggtgcgaa caatggattc cgggaagaca cgttcaccgg gaaccggggg
    57001 gtcaactttg gtaatcgaca gtgggcgata aatgccgtag ctgccgatag ttgcgtagac
    57061 tcgcgctaac tgtaacggtg ttaccattag cccgtagccg aaagagaagg tggccctctc
    57121 tatgtcagac caccgttgtt tttgaggata taagccactg cgttctccga ccaaccccaa
    57181 attggtcgct tttcccagtc caaaacgtga gtaagtatct actaacgctg aggacggcat
    57241 cgctaacgcc agcttggaaa caccgacgtt actcgacttc tgtaataccc cggtcagggt
    57301 taattcgctg tagcgtgcca cgtctttgat ttcgtggccg ttaattcgat aaggaatggt
    57361 attgagtacc gagttttccc gcaccacgcc acgttgcaac gcggtcatca ccaccatcgg
    57421 tttaaccgtt gagcccggtt caaacacgtc ggtgatggta cggttacgca tcgcctcttt
    57481 cggcgtgccg ctcagattgt tagggttgta tgacgggctg ttagccatcg ccagcacttc
    57541 acctgtgttg acatccacca gcacggcgct accagattca gccttgttaa aggccaccgc
    57601 gttgttcagt tcgcgataaa ccagcgcctg caggcgttca tcaatactca gcgccaggtt
    57661 gtgcgctgcc tggctgtcag tagaagaaat atcttcaatt acgcgaccat agcggtcttt
    57721 acgcacaatg cgctcacccg gctgcccggt aagccattta tcgaaactct tctcaacgcc
    57781 ctcaatccct tgactatcga cgttagtaaa gccgatgagg tgagcagtca cttcgccgga
    57841 cggatagtaa cggcgagact cttcacgcag atgaatcccc ggcagtttca gttttttgat
    57901 gtagtccgcc atgtcagggt ttacctgacg cgccagataa ataaagcgcc ctttcgggtt
    57961 ggcgttaatg cgggcagaaa gctgatccag cggaatattg agcgcgttag ccagcgcctt
    58021 ccagcggtca ccgacgctga taccgccagc gtcatgcact tctttcgggt cagcccaaat
    58081 cgcttttacc ggcacgctca ccgctaacgg gcgaccagaa cggtcagtaa tcatgccgcg
    58141 ggaggtggaa acttgctgaa cgcgaagaga acgcatgtcg ccctctttca ccagcatatc
    58201 cggggagata acttgtaacc acgctacgcg tccgagcaga aaagccagcg ccaggagaat
    58261 acagccgcat aacaacgcaa aacgccaact gataaagttg gcatgttctt cctgacgttt
    58321 tggtttctgc gttttcgccg ctgctttcat gcgtcgcgtt tatccttatt tttgcactac
    58381 gatattttct tgtgacggat caacatgctg catttgcagc ttttccgtgg cgatcctttc
    58441 cacccggcta tggtcgccga gcgcattctc ttcaaggatc aggttgcgcc attcaatgtc
    58501 taaagcatct cgctccagca ccagttgttc gcgctgagcg gtcagtaaac gggtatggtg
    58561 cgccgtggtt accacggtca ccgccgtcaa aataatgcaa atgaacaggc agagtggcag
    58621 cttcccaaat cgcaaaagat cgtcaccgat aacaccaggc aatgcatggc gctcgtggct
    58681 tcccatcgat cctttaactt tgcttagagc ttctgtcact ctgctgatca tgcgttcgtc
    58741 ctctctgcaa tacgcagaac tgaactacgg gcacgagggt tctcagccac ctcttcttcg
    58801 cccggcatta acttgcctag tgctcgcagc tgacggccac ccaatttttt gagctgctct
    58861 tcagtcatcg gtaaccctgc cggaacttgc ggaccgcggc tgttttcacg cataaaacgt
    58921 ttcacaatac ggtcttccag cgagtggaag ctgatgatcg aaagccgccc acccggggcc
    58981 agcacgttga gcgagctttt tagcgcctgc tctatctcct ccagttcact gtttacccaa
    59041 atgcgcaccg cctggaaggt acgggtcgcg ggatgtttaa acttatcttt caccggcgtt
    59101 gcagcagcca cgacttccgc cagttctttg gtgcgggtca tcggctgttc gcggttacgc
    59161 tcgacaatgg cgcgggcaat gcgtttggca aaacgctctt caccataggt tttcaatacc
    59221 caggcgatat cggcttcttc tgcggtttgt agccattcag cggctgactg cccacgggtt
    59281 gggtccatac gcatgtccag cggaccatcg cgcataaagg aaaagccgcg ttcagcatca
    59341 tcaagttgcg gtgaagagac gccaagatcg aggagaatgc cgtcgatctt gccaataaga
    59401 tcgcgctcgg caacgtattc gcccagcgcg gagaaaggtc cgtggatgat ggagaagcgc
    59461 ggatcatcaa tagtcttcgc aacggcgata gcctgcgggt cgcgatcgat cgccagcaaa
    59521 cgcccctctt cgccaagctg cgagaggatc agacgtgagt gaccaccgcg accaaaagtc
    59581 ccatcaatgt agatgccatc aggacggata ttgaggccat taacggcttc atccagcagc
    59641 accgtagtat gtttatagtt ttccatcatt ttatagagac aagtcctgca gtcgctccga
    59701 taagtctccg gtagccaact gctctgcgtc gatatcttcc ttgacctgtt gatgccaggt
    59761 tgtttcatcc cacagctcaa acttgttgaa ctgtccaacc agcatcactt cttttgtcag
    59821 cccggcatgt tgccgcagta ctggcgcgat taacaatcga cctgcgccat ccatctgaca
    59881 ttcgctggca tgacctaaca gtaggcgctg cacacggcgc tcaaccgggt tcatgctcga
    59941 cagacgcgat aatttttgct cgataatttc ccattcaggc agggggtaaa gcagcaggca
    60001 cgggtgatga atgtcaatgg tgcaaaccat ttgaccggca gcgttctcaa gcagctgttc
    60061 ccgataacgg gtaggcactg ataagcgccc tttgctgtcg agattgacta acgttgctcc
    60121 ccggaacatg ccagcctcac cccttattcc cactttgccc cacaatttcc cactgaaagg
    60181 agtttacgga gcgtaggaaa agcttgtcaa gccagtcaca aggcataccg aggtaaaaaa
    60241 cccgatgttt acggctaata tcagcgttgt ttattttacg tccaacgtgc gaagcaaatt
    60301 aacgttatga atagctgtaa gaaaaaatcc ctcatcgtca tcactgtttt aaaatgactc
    60361 actattttcc acagcaaata taaagtgtca gtttgcgacg cgagcggcat tttaggacat
    60421 atcttccccg gttaacagtc cttgttacgt ctgtgtggcg cttgatgcca gcgggtttgc
    60481 gcaaaagcaa agaaaagtgt ttgttaaatc ggcaattcat cgccgcatgt aacaaaataa
    60541 tgcaaaaacg cgctgtcgat atcacttaat tattgtgaat ttcagctggt gagttaatt
//
LOCUS       DBKEKI010000008        59258 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.008, whole genome shotgun sequence.
ACCESSION   DBKEKI010000008 DBKEKI010000000
VERSION     DBKEKI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.008"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1166)
                     /locus_tag="ACUQPX_000478"
     CDS             complement(<1..1166)
                     /locus_tag="ACUQPX_000478"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HHV9055278.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGG"
     gene            complement(1178..1465)
                     /gene="eutN"
                     /locus_tag="ACUQPX_000479"
     CDS             complement(1178..1465)
                     /gene="eutN"
                     /locus_tag="ACUQPX_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HHV9055279.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(1572..1865)
                     /gene="eutM"
                     /locus_tag="ACUQPX_000480"
     CDS             complement(1572..1865)
                     /gene="eutM"
                     /locus_tag="ACUQPX_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HHV9055280.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(1904..2920)
                     /gene="pta"
                     /locus_tag="ACUQPX_000481"
     CDS             complement(1904..2920)
                     /gene="pta"
                     /locus_tag="ACUQPX_000481"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HHV9055281.1"
                     /translation="MAVWLQIELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLAHAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEMNRESSLQ
                     TLVE"
     gene            complement(2948..3283)
                     /gene="eutT"
                     /locus_tag="ACUQPX_000482"
                     /pseudo
     CDS             complement(2948..3283)
                     /gene="eutT"
                     /locus_tag="ACUQPX_000482"
                     /inference="COORDINATES: protein motif:HMM:NF011595.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
     gene            complement(3280..3981)
                     /gene="eutQ"
                     /locus_tag="ACUQPX_000483"
     CDS             complement(3280..3981)
                     /gene="eutQ"
                     /locus_tag="ACUQPX_000483"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HHV9055282.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(3956..4435)
                     /gene="eutP"
                     /locus_tag="ACUQPX_000484"
     CDS             complement(3956..4435)
                     /gene="eutP"
                     /locus_tag="ACUQPX_000484"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HHV9055283.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(4448..4783)
                     /gene="eutS"
                     /locus_tag="ACUQPX_000485"
     CDS             complement(4448..4783)
                     /gene="eutS"
                     /locus_tag="ACUQPX_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HHV9055284.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(5076..7355)
                     /gene="maeB"
                     /locus_tag="ACUQPX_000486"
     CDS             complement(5076..7355)
                     /gene="maeB"
                     /locus_tag="ACUQPX_000486"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HHV9055285.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            7644..8594
                     /gene="tal"
                     /locus_tag="ACUQPX_000487"
     CDS             7644..8594
                     /gene="tal"
                     /locus_tag="ACUQPX_000487"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HHV9055286.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            8614..10617
                     /gene="tkt"
                     /locus_tag="ACUQPX_000488"
     CDS             8614..10617
                     /gene="tkt"
                     /locus_tag="ACUQPX_000488"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HHV9055287.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     NPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(10720..11763)
                     /locus_tag="ACUQPX_000489"
     CDS             complement(10720..11763)
                     /locus_tag="ACUQPX_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HHV9055288.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(11889..12464)
                     /gene="nudK"
                     /locus_tag="ACUQPX_000490"
     CDS             complement(11889..12464)
                     /gene="nudK"
                     /locus_tag="ACUQPX_000490"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HHV9055289.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(12532..14511)
                     /gene="aegA"
                     /locus_tag="ACUQPX_000491"
     CDS             complement(12532..14511)
                     /gene="aegA"
                     /locus_tag="ACUQPX_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HHV9055290.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLAFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAERRHAAQGIIDWLGVKSVKSH"
     gene            14716..16428
                     /gene="narQ"
                     /locus_tag="ACUQPX_000492"
     CDS             14716..16428
                     /gene="narQ"
                     /locus_tag="ACUQPX_000492"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HHV9055291.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGKPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPPLALDAQMQVHLLQIIREAVLNAMKHANASEIAISCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEGKGNT
                     PAEV"
     gene            16630..19743
                     /gene="acrD"
                     /locus_tag="ACUQPX_000493"
     CDS             16630..19743
                     /gene="acrD"
                     /locus_tag="ACUQPX_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HHV9055292.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(19842..19901)
                     /gene="ypfM"
                     /locus_tag="ACUQPX_000494"
     CDS             complement(19842..19901)
                     /gene="ypfM"
                     /locus_tag="ACUQPX_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HHV9055293.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            20282..20638
                     /locus_tag="ACUQPX_000495"
     CDS             20282..20638
                     /locus_tag="ACUQPX_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HHV9055294.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            20642..21769
                     /gene="dapE"
                     /locus_tag="ACUQPX_000496"
     CDS             20642..21769
                     /gene="dapE"
                     /locus_tag="ACUQPX_000496"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HHV9055295.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            21797..21997
                     /locus_tag="ACUQPX_000497"
     CDS             21797..21997
                     /locus_tag="ACUQPX_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HHV9055296.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22107..22805)
                     /gene="ypfH"
                     /locus_tag="ACUQPX_000498"
     CDS             complement(22107..22805)
                     /gene="ypfH"
                     /locus_tag="ACUQPX_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HHV9055297.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22879..24894)
                     /gene="tmcA"
                     /locus_tag="ACUQPX_000499"
     CDS             complement(22879..24894)
                     /gene="tmcA"
                     /locus_tag="ACUQPX_000499"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HHV9055298.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(24909..25772)
                     /gene="ypfJ"
                     /locus_tag="ACUQPX_000500"
     CDS             complement(24909..25772)
                     /gene="ypfJ"
                     /locus_tag="ACUQPX_000500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HHV9055299.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNHLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(25940..26653)
                     /gene="purC"
                     /locus_tag="ACUQPX_000501"
     CDS             complement(25940..26653)
                     /gene="purC"
                     /locus_tag="ACUQPX_000501"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HHV9055300.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(26866..27900)
                     /gene="bamC"
                     /locus_tag="ACUQPX_000502"
     CDS             complement(26866..27900)
                     /gene="bamC"
                     /locus_tag="ACUQPX_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HHV9055301.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(27917..28795)
                     /gene="dapA"
                     /locus_tag="ACUQPX_000503"
     CDS             complement(27917..28795)
                     /gene="dapA"
                     /locus_tag="ACUQPX_000503"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HHV9055302.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNVSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            28941..29513
                     /locus_tag="ACUQPX_000504"
     CDS             28941..29513
                     /locus_tag="ACUQPX_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HHV9055303.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKSTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29513..29983
                     /gene="bcp"
                     /locus_tag="ACUQPX_000505"
     CDS             29513..29983
                     /gene="bcp"
                     /locus_tag="ACUQPX_000505"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HHV9055304.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            30236..30853
                     /gene="hyfA"
                     /locus_tag="ACUQPX_000506"
     CDS             30236..30853
                     /gene="hyfA"
                     /locus_tag="ACUQPX_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HHV9055305.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            30853..32870
                     /gene="hyfB"
                     /locus_tag="ACUQPX_000507"
                     /pseudo
     CDS             30853..32870
                     /gene="hyfB"
                     /locus_tag="ACUQPX_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            32881..33828
                     /gene="hyfC"
                     /locus_tag="ACUQPX_000508"
     CDS             32881..33828
                     /gene="hyfC"
                     /locus_tag="ACUQPX_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HHV9055306.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            33845..35284
                     /gene="hyfD"
                     /locus_tag="ACUQPX_000509"
     CDS             33845..35284
                     /gene="hyfD"
                     /locus_tag="ACUQPX_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HHV9055307.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLLGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            35296..35946
                     /gene="hyfE"
                     /locus_tag="ACUQPX_000510"
     CDS             35296..35946
                     /gene="hyfE"
                     /locus_tag="ACUQPX_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HHV9055308.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            35951..37531
                     /gene="hyfF"
                     /locus_tag="ACUQPX_000511"
     CDS             35951..37531
                     /gene="hyfF"
                     /locus_tag="ACUQPX_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HHV9055309.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMLHEV
                     ARGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGILKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            37521..39235
                     /gene="hyfG"
                     /locus_tag="ACUQPX_000512"
                     /pseudo
     CDS             37521..39235
                     /gene="hyfG"
                     /locus_tag="ACUQPX_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            39245..39799
                     /gene="hyfH"
                     /locus_tag="ACUQPX_000513"
     CDS             39245..39799
                     /gene="hyfH"
                     /locus_tag="ACUQPX_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HHV9055310.1"
                     /translation="MLKLLKTIMRAGTTTVKYPFAPLEVSPDFRGKPDLMPSQCIACG
                     ACACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTV
                     THKADLYYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVC
                     PECKQRATLINDDTDVPLVAKEQL"
     gene            39796..40554
                     /gene="hyfI"
                     /locus_tag="ACUQPX_000514"
     CDS             39796..40554
                     /gene="hyfI"
                     /locus_tag="ACUQPX_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HHV9055311.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            40547..40960
                     /gene="hyfJ"
                     /locus_tag="ACUQPX_000515"
     CDS             40547..40960
                     /gene="hyfJ"
                     /locus_tag="ACUQPX_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HHV9055312.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            40990..43002
                     /gene="hyfR"
                     /locus_tag="ACUQPX_000516"
     CDS             40990..43002
                     /gene="hyfR"
                     /locus_tag="ACUQPX_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HHV9055313.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEELLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCTAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLISWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGHRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            43024..43872
                     /gene="focB"
                     /locus_tag="ACUQPX_000517"
     CDS             43024..43872
                     /gene="focB"
                     /locus_tag="ACUQPX_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HHV9055314.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAILHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(43910..44971)
                     /locus_tag="ACUQPX_000518"
     CDS             complement(43910..44971)
                     /locus_tag="ACUQPX_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HHV9055315.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            45184..46647
                     /gene="bepA"
                     /locus_tag="ACUQPX_000519"
     CDS             45184..46647
                     /gene="bepA"
                     /locus_tag="ACUQPX_000519"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HHV9055316.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            46668..47027
                     /gene="arsC"
                     /locus_tag="ACUQPX_000520"
     CDS             46668..47027
                     /gene="arsC"
                     /locus_tag="ACUQPX_000520"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HHV9055317.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(47160..47861)
                     /locus_tag="ACUQPX_000521"
     CDS             complement(47160..47861)
                     /locus_tag="ACUQPX_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HHV9055318.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(47956..49245)
                     /gene="uraA"
                     /locus_tag="ACUQPX_000522"
     CDS             complement(47956..49245)
                     /gene="uraA"
                     /locus_tag="ACUQPX_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HHV9055319.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(49331..49957)
                     /gene="upp"
                     /locus_tag="ACUQPX_000523"
     CDS             complement(49331..49957)
                     /gene="upp"
                     /locus_tag="ACUQPX_000523"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HHV9055320.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            50281..51318
                     /gene="purM"
                     /locus_tag="ACUQPX_000524"
     CDS             50281..51318
                     /gene="purM"
                     /locus_tag="ACUQPX_000524"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HHV9055321.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKCHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            51318..51956
                     /gene="purN"
                     /locus_tag="ACUQPX_000525"
     CDS             51318..51956
                     /gene="purN"
                     /locus_tag="ACUQPX_000525"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HHV9055322.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            52128..54194
                     /gene="ppk1"
                     /locus_tag="ACUQPX_000526"
     CDS             52128..54194
                     /gene="ppk1"
                     /locus_tag="ACUQPX_000526"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HHV9055323.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            54199..55740
                     /gene="ppx"
                     /locus_tag="ACUQPX_000527"
     CDS             54199..55740
                     /gene="ppx"
                     /locus_tag="ACUQPX_000527"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HHV9055324.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEDAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(55779..58022)
                     /gene="pdeF"
                     /locus_tag="ACUQPX_000528"
     CDS             complement(55779..58022)
                     /gene="pdeF"
                     /locus_tag="ACUQPX_000528"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HHV9055325.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHIFSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRILGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRCGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(58204..58356)
                     /locus_tag="ACUQPX_000529"
     CDS             complement(58204..58356)
                     /locus_tag="ACUQPX_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055326.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            58374..58565
                     /locus_tag="ACUQPX_000530"
     CDS             58374..58565
                     /locus_tag="ACUQPX_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HHV9055327.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            58876..>59258
                     /locus_tag="ACUQPX_000531"
     CDS             58876..>59258
                     /locus_tag="ACUQPX_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HHV9055328.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTY"
BASE COUNT        13875 a        15694 c        15270 g        14419 t
ORIGIN      
        1 ccgccctcca gtttcaccgc cagcgcaatg gcatccgcca cgttggcgac gcgcacgacg
       61 ggcagcaccg gcatcatcag ttcggtcacg gcaaacggat gttctgcagt ggtttccaca
      121 aacagcaaac gcgtttcttg cggcacgttc aggccgattg ccgccgcgat tttcgccgca
      181 tcgcgaccaa cccagtcacg gctgacggtg ccttttccgc gctcgtcgat atttttcagc
      241 aacaccggtt gcagctgctg cgcctgttct gcggtcagtt tcaccgcgtg ctggccttcc
      301 atcagacgca tcagttcatc ggctacgcta tcaacaacaa tcagtacctt ttcgtcggca
      361 caaatgatgt tgttatcgaa agacgcgcct ttgacgatgg actgagcagc acgggcgagg
      421 tcagcggttt catccaccac taccggcggg ttgccagcgc ctgcggcaat cagacgttta
      481 ttggtgtgtt tacgcgccgc ttctactacc gcttcgccgc cggtcaccac cagcaggccg
      541 atacccggaa acttgaacaa gcgttgcgcg gtttcgatat ccggatttgc cacagtaacc
      601 agtaagtttt ccggcccacc tgcggcaaca atcgcctggt tgagcagcgt aatcgcccgc
      661 tgggagactt ttttcgccgc cggatgcggg gcaaaaatga cgctgttgcc cgcggcaatc
      721 aggctgatgg cgttgttaat tacggttgcc gccgggttag tggaaggcgt caccgaagcc
      781 accacgcccc agggtgcgtt ttcaattagg gtcaggccgt tgtcgccagt cagcacttgc
      841 ggagagaggc actcaacgcc tggtgtgccg cgcgcctgag cgacgttttt tgcaaattta
      901 tcttcaacgc gccccatgcc ggtttcactg acggcaagtt ccgctaaatc tctggcgtgt
      961 ttttcgcctg cttcacgaat ggcagcaatg gctaactggc gcattgccac gctttttaac
     1021 ccttgctggg cgactttggc tgccgcaacg gcgtcatcca gggacgcgaa aacgcccatc
     1081 tcatgaacag cggcgggcgg cgtgtcactg ctttgcattt tcagcagtac cgctttcacc
     1141 acctgttcaa tatcctgttg attcatgatg ttctgcctta tttgtggaaa attacctgac
     1201 cgccagacac cacctcatcg acgatgccaa tcacgcacag atcgaccggt gacgtttcgc
     1261 ttttatgcgc ctggcgggcg gaactgccac tcaccagcaa cacccactcc ccggttcccg
     1321 cgccaatatt gtcgatggcg acggcacatt gcccgtcagg attaccttgt ggatcaatca
     1381 tttccaccat cagcaatttg tcatgcgcca gtccgtgatg gcgtacggta caaacaattt
     1441 gtccagtgac gactgccagt ttcatacccg cctccgtggc gtatttcagg taaaagctcc
     1501 ccctaccctc cgcagaaggt aaaatgaaaa aggagagagc gtgacgcccg aatcgacgtc
     1561 acacagggtg attacaggtt gctgctatcg cctttcaggc cgatcgggaa cacttcttcc
     1621 aggtcgccgt gcggacgcgg gataacgtgt acagatacca gctcgccgat acgctgtgcc
     1681 gctgcggcac cggcatcggt cgccgcttta caggctgcaa catcaccacg aaccatggca
     1741 gtacacaggc cgccgccaat ctgcttaaca ccaaccagct tgacgcgcgc agctttcacc
     1801 atcgcgtcag aagcctcaat cagtgcaacc aggccccggg tttcgatcat tcctaatgct
     1861 tccattgtgc tttcctcttt atcagggtcc agaacgggac cgttcattca accagtgttt
     1921 gtaaactgct ttcgcggttc atttctgtct gacgcggcac agctgccacc agcgccagct
     1981 cgataatttc ctgcacgcta caaccacgag agagatcgtg catcggcgcg gcaagtcctt
     2041 gtatcaatgg cccgacggca cgatatccgc cgagtcgttg tgcgattttg tagccaatat
     2101 ttccggcttc cagcgacgga aaaaccatca cattggcttt gccctgtagc gggctggcag
     2161 gcgctttttg cgccgccact tccggcacga aggcggcgtc aaactgcaac tcgccatcaa
     2221 ccaccagctt tggtgcgcgc tcacggacga tttctgtcgc ctgctggacg ttggcaacgc
     2281 aggggtgacg ggcgctaccg ttactggaaa acgacagcat cgccacgcgc ggctcttctc
     2341 cggtgagggc gcgccaggtt tcggcactgg caagcgcgat atccgccagt tgcgccgccg
     2401 tcggctgtgg caccacgctg caatcggcaa agcccaacgc cggaccgctg tactgtggca
     2461 gcatcaggaa aatggatgag agcgttttac agcctggctg caagccgata atgcgtaatc
     2521 cggcacgcag cacattcgcc gtggaagaga ggttgcccgc gatacagaca tccgctttac
     2581 cggcgctgac cattgctgcg gcgaacatca gcggatcggt aagtttttcc agcgcatccg
     2641 gcggcgtttt ttcgcccgcg tgggccagcc agcgatgagc aaattcttcc cgcattgcga
     2701 ggttgccatg cggatcaatc acctgtagcc cgtccatcgc cacgccgtga ctgagtgcaa
     2761 actgacgaag ttcaaacgga ttagcgacca gaatgggcgt tgccagacct tgttgatgta
     2821 aatattgcgc agctttcagc acacgttgat ctaacgcatc cggaaaaacc actctggcgg
     2881 gcgctcgcaa cgccagttca atttgcagcc acactgccag cgcaatggtg ctgtcgagaa
     2941 ccgcgcgaaa acccagacgc ggatcgcttt tggcgaccat tttttccgcc gacaggtgag
     3001 tcagcgtatc cggctttttt gccaccagct ggcggcacag ttcgcagcac gcctgcggat
     3061 gttcatcgct actagtcagc ccatgaacgg gctgcggctg ctgctgttca tcgtcaacaa
     3121 acaggcggcc ctgctcgtca ataaacttga tgcgcagatg gcggctttcc agcaactccc
     3181 gggcagaggg cgtcaggcga ctgtccgcag ggagatggat ctctgcgcct tcgctgagcg
     3241 tatggttcgc tcttagccat gcttcggtga tgaaatcttt catagggatt gccagttagc
     3301 cggccaggcg acatacagga atttcacgct tgatgtcgta ccaaattcga tgctggagcc
     3361 tttcgggata aacatcacgt cgcctgcttt ggcgatcatg gtttcgcctt cgtggcgaac
     3421 atgcagctcc ccttccagca ccatgtcgat ttcgtcgtag ttcagggtcc acgggaagaa
     3481 tgcgttctcc cactgcataa aaccggcggc catgctgctg ccgtcgtctc cggtcacaag
     3541 atcagtcaag cccacgcaat gcggctctgc gccgtcgaaa cgaccaaact tgacgctgct
     3601 gccatcaatc actttgatgc cgcctttgcc ggtcaccgat ttgaagctcg gctgcattgc
     3661 gccctgctcc agcgactgtt tttccttcat cactttttcc atcagctgtg cgaccaggct
     3721 ttcggtaaac tggccttccg gcagctgggc aatgatggtt tcgcggatgc gctggctttc
     3781 ggttttgtcg gctggcacgc tggcgggcac ggacgccgtt accgggatcg attcatcaca
     3841 ttcggtgatg gtaaaaccca gtagatccgc cacttcacgg gcttccgggg taataatgct
     3901 ggcgcgcaga accacggaca ttgcctgttc gccgcgcgcg tgtgcttcac gaatatcatt
     3961 cgctgtgatg agttttttca cctgcttcct cctgtttggt gagagatgcc agataatcca
     4021 ccagttgctg cacgctttgc gggtcgtggc tgtttagctc gaaaatcggc tcttcgaaac
     4081 ccgtctcaag caataacttt cgcgttgcgg cgacgtcagc atctggcatg tccgttttac
     4141 tgatgacggc gatttgccgt ttgctgacgc caatatccag caacccggca ggcaaccgac
     4201 tttccgggtc atttgcgcca tggacataga tcagcatgtc tacatcctgt agcgtggtaa
     4261 ttaaggcgtg ataccagcgg ggatggctaa aatattcacc cggcgtatca atatcgccct
     4321 tatcattaaa ttccacggcc tgtgtttttc tggcgagggt ataattcccc tgcaacgcat
     4381 taaataacgt tgtttttcct gccccgacgc tacccacaaa cgcaattcgt ttcatgaatg
     4441 cctcgtatta acttttggtc atttcacaaa gcgtataatt taataagcga cctaaaccgc
     4501 tgacggtttg tgataacgct tcttctactg cgcccaccga gccataaatc accaatgcac
     4561 cgctgaagcg gtcgagaaaa ccgatatgca catcagcggc tttcagcgct aaatcaccgg
     4621 cgatcatcgc cgtttcgccg ggagttagcg tcataatgcc aattgcgccc gcatccggaa
     4681 cgccgatttt cttcgcaagt tcctcaccgg gatgcgcgat gagatgcgcc agtgtgacct
     4741 gtttgccggg cacaaattcc tgaatgatgc gttctttatc catgagtcgc cgcctgtgaa
     4801 aaactttcgt gtatcgtgac aatttttcgt cagagtaaat aacaaaattc ggcaagcgcg
     4861 tttaaaaggt gaggtagatc actaagaatg gagagatatt tactgttatg aataaacttt
     4921 catatgcgac gcatgaatat aaataaaata atatcgagat attttatata ctgatttttt
     4981 tattattcgc aaactcagta ataagctttg ctaatatatt tcaggaaaac cgcttaccct
     5041 gaatattcag ggtaagcgtg agagttaaga atgaattaca gcggttgggt ttgcgcttca
     5101 accacggcca gcgccaccat attgacgata cgacgcaccg atgcgatcgg cgttaacacg
     5161 tgaaccggtt tcgccacgcc catcagcacc ggaccgacag tcacaccttc cgagctggaa
     5221 acgcgcagta agttgtaact aatgcgggca gcttccatgt tcggcatcac cagaatattg
     5281 gcagaacctt tcaaagggct gtccggcata cggtcgttgc gaatcgcttc caccagcgct
     5341 gcatcgccgt gcatttcacc atcaatcatc agatctggcg cacgatccct gaccagttcc
     5401 agcgcctgac gcattttgct cgacgacggg cagtcagaag aaccaaagtt ggagtgcgac
     5461 aacagagcaa cgcgcggctc aataccaaaa cgacggacag tttctgccgc catcaaggtg
     5521 atctccgcca gctcttctgc gtctggttcg tcattgacgt aggtatcggc aataaaggtg
     5581 ttaccactcg gcagcagcag cgcgttcatt gcacctgcgg tgtgaacgcc atcgcgataa
     5641 ccaaagacat ttttcaccac gctaaaatgt tcatgataat cgcccaccgt accgcagatc
     5701 attgcatcgg cttccccacg ctgaaccatg atcgcgccga tcactgtcgg gttactgatc
     5761 agcgcacgct gcgcctgttc ctgagtgaca ccgcgacgct tcatgatctg gaagtattcg
     5821 gtccagtact ctttaaagcg cggatcggat tcgttattga cgatttcaaa atcaacgccc
     5881 gctttgatct gcaagcccag tttctgaatg cgcatttcga tcacgttcgg acgaccgata
     5941 aggatcggtt tcgccagtcc cagcgttacc agttcctgag tcgcatgcag aacgcgcgcc
     6001 tcttcccctt ccggcagaac tacgcgcttc ggcgctttgc gagcctggga gaaaatcggc
     6061 ttcataaaca ggttggtttt gtaaacgaac tcagtcagct tgtcgatgta gacgtcgaaa
     6121 tcagcaatcg gacgcgtcgc cacgcccgac tccatcgccg cttttgcgac cgcaggagcg
     6181 atcttaacga tcaagcgcgg atcaaacggt tttggaatga tgtattccgg accaaagctc
     6241 agatcctgaa cgccatacgc tgaagccacc acttcactct gttctgcatg ggccagttct
     6301 gcaatcgcgc gtaccgccgc cagtttcatc tcttcgttga tggcggttgc gccaacgtcc
     6361 agcgcgccac ggaagatgaa cgggaagcaa agaacgttgt tcacctggtt cgggtagtcg
     6421 gaacggccgg tacagatgat ggcatccgga cgcacttctt tcgccagcgg cggcagaatt
     6481 tccggttccg ggttcgccag cgccaggatc attggtgccc gagccatttt cttcaccatt
     6541 tcctgggtca gcactttcgg gccggaacag cccaggaaaa tatccgcacc ttcaatcaca
     6601 tcatcgaggg tacgtttgcc gtcatccacc accgcatatg cggctttggt ttccgccata
     6661 tttggctcac ggccctgata gataacgcct tttgaatcgc aaaccacgat gttatgtttt
     6721 tgcagcccca gcgctaccag caggttcata caggcgattg ctgcggcacc cgcgccggaa
     6781 accaccatcc gcacgtcgga gatgtttttc tccaccacgc gcaagccgtt gaggatggcg
     6841 gcagtgctga tgattgccgt gccgtgctga tcgtcgtgga ataccggaat attcatccgc
     6901 tcgcgcagtt tctgttcaat atagaaacat tctggcgctt taatgtcttc gaggttgatg
     6961 ccgccgaagg tcggttcgag cgcggcgacg acttcaataa atttgtccgg gtcgagttca
     7021 tcaacttcaa tgtcaaatac atcaatccca gcgaatttct taaatagaac gcccttgcct
     7081 tccatcaccg gtttgcctgc cagcgcgcca atgttgccta accccagcac cgccgtaccg
     7141 ttagagatca ccgcaaccag gttaccgcgc gcggtatatt tgtaggcttt tagcgggtct
     7201 ttttcgattt caagacaagg tgcggcaacg cctggtgagt aggccagcgc cagatcgcgc
     7261 tgcgttgcca gaggcttggt tggagaaacc tggattttcc ctggaactgg aaattcatgg
     7321 aaatcaagtg cactttgttt taactggtca tccatttggt tgttcctttc acgtaacgtt
     7381 cacaaataaa gtgtgtgggc aacagcccct gcccacaacg tggcgcacat tattaccctg
     7441 ccggagtcta cagactttga gcaagtccaa actctcacca ttaatataat gttttggtaa
     7501 taatcctata acactgatgt tacctgctta atccagcaat accatgcctg tctgctatgc
     7561 ttttttgatg cgtttagcga aatttctcag aagtgtgaat taatgcactc atctaacact
     7621 ttacttttca aggagtattt cctatgaacg agttagacgg catcaaacag ttcaccactg
     7681 tcgtggcaga cagcggcgat attgagtcca ttcgccatta tcatccccag gatgccacca
     7741 ccaatccttc gctgttactc aaggctgccg gattatcaca atatgagcat ttaatagacg
     7801 atgctatcgc ctggggtaaa aaaaatggca agacccagga acaacaggtg gtcgcagcgt
     7861 gtgacaaact ggcggtcaat ttcggtgctg aaattctcaa aatcgtaccc ggttgcgtgt
     7921 caacagaagt tgatgcacgc ctctcttttg ataaagaaaa gagtattgag aaggcgcgcc
     7981 atctggtgga cttgtatcag caacaaggcg ttgagaaatc acgcattttg atcaaactgg
     8041 cttcgacctg ggaaggaatt cgcgcggccg aagagctgga aaaagaaggt attaactgca
     8101 acctgacgct gctgttttct tttgcccagg cgcgggcctg tgcggaagca ggcgtttttc
     8161 tgatttcgcc gtttgtaggg cgtatttatg actggtatca ggcacgcaag ccgatggacc
     8221 cgtatgtggt ggaagaagat ccgggcgtta aatcggtgcg caatatctac gactactata
     8281 agcaacacca ctatgaaacc attgtgatgg gcgcgagctt ccgtcgcacc gaacaaatcc
     8341 tcgccttaac cggctgcgat cgactgacta tcgcaccgaa tttgttgaag gagctgcagg
     8401 aaaaagtttc gccagtggta cgtaaattaa tcccgccttc tcagacgttc ccacgcccag
     8461 cacccatgag cgaagcggag ttccgttggg agcacaatca ggatgcgatg gcggtagaaa
     8521 aactgtctga aggcattcgt ctgttcgctg ttgatcaacg caaactggaa gatcttcttg
     8581 ccgccaaact ataaaccagc cacggagtgt tatatgtccc gaaaagacct tgccaatgcg
     8641 attcgcgcac tcagtatgga tgcggtacaa aaagccaatt ctggtcatcc cggcgcgccg
     8701 atgggcatgg ctgatattgc cgaagtgctg tggaacgatt ttcttaaaca taatcctacc
     8761 gacccaacct ggtatgatcg cgaccgcttt attctttcca acggtcacgc gtcgatgctg
     8821 ctctacagtt tgctacatct gaccggttac gacctgccgc tggaagaact gaagaacttc
     8881 cgtcagttgc attcgaaaac cccaggccac ccggagattg gctatacgcc aggcgttgaa
     8941 accaccaccg gcccgcttgg acaaggtttg gcgaacgccg tcgggctggc gatagcagag
     9001 cgtacactgg cggcgcagtt taaccagcca gaccatgaga tcgtcgatca cttcacctat
     9061 gtgtttatgg gcgacggctg cctgatggaa ggtatttccc acgaagtctg ttcgctggca
     9121 ggcacgctgg gactgggcaa gctgattggt ttttacgatc acaacggtat ttccatcgac
     9181 ggtgaaacag aaggctggtt taccgacgat acggcaaaac gttttgaagc ctatcactgg
     9241 catgtgatcc atgaaatcga cggtcacaat ccgcaggcgg tgaaggaagc gatccttgaa
     9301 gcgcaaagcg tgaaagataa gccgtcgctg attatctgcc gtacggtgat tggctttggt
     9361 tcgccgaata aagcaggtaa ggaagaggcg cacggcgcac cactggggga agaagaagtg
     9421 gcgctggcac ggcaaaaact gggctggcac catccgccat ttgagatccc taaagagatt
     9481 tatcacgcct gggatgcccg tgaaaaaggc gaaaaagcgc agcagagctg gaatgagaag
     9541 tttgccgcct ataaaaaggc tcatccgcaa ctggcagaag agtttacccg acggatgagc
     9601 ggtggtttac cgaaggactg ggagaaaacg actcagaaat atatcaatga gttacaggca
     9661 aatccggcga aaatcgctac ccgtaaggct tcgcaaaata cgcttaacgc ttacgggccg
     9721 atgctgcctg agttgctcgg cggttcggcg gatctggctc ccagcaacct gaccatctgg
     9781 aaaggttctg tttcgctgaa ggaagatccg gcgggcaact acattcacta cggggtgcgt
     9841 gaatttggca tgaccgctat cgccaacggc atcgcgcacc acggcggctt tgtgccgtat
     9901 accgcgacgt tcctgatgtt tgttgaatac gcccgtaacg ccgcgcggat ggcggcactg
     9961 atgaaagcgc ggcagattat ggtttatacc cacgactcaa ttggcctggg cgaagatggt
    10021 ccgacgcacc aggctgttga gcaactggcc agcctgcgct taacgccaaa tttcagcacc
    10081 tggcgaccgt gcgatcaggt ggaagcggcg gtgggctgga agctggcggt tgagcgccac
    10141 aacggaccga cggcactgat cctctcaagg cagaatctgg cccaggtgga acgtacgccg
    10201 gatcaggtta aagagattgc tcgtggcggt tatgtgctga aagacagcgg cggtaagcca
    10261 gatattattc tgattgccac cggttcagag atggaaatta ccctgcaagc ggcagagaaa
    10321 ttagcaggag aaggtcgcaa tgtacgcgta gtttccctgc cctcgaccga tattttcgac
    10381 gcccaagatg aagaatatcg ggagtcggtg ttgccttcta acgttgcggc tcgcgtggcg
    10441 gtggaagcag gtattgccga ttactggtac aagtatgttg gtctgaaagg ggcaattgtc
    10501 gggatgacgg gttacgggga atctgctccg gcggataagc tgttcccgtt ctttggcttt
    10561 accgccgaga atattgtggc aaaagcgcat aaggtgctgg gagtgaaagg tgcctgatgg
    10621 tgatcgccgg atgctgtatg ttcccctcac cctaaccctc tccccagaag ggcgagggga
    10681 cggtccgtgc gctttttagg gccgcatctg cgattttgtt taacgggtga tccacaacgt
    10741 gggccaggcg tctggcccat gccagttatc gcaggtgggt tctgcggcgt aacgcaccag
    10801 gcgaaaacgc tgaccgtcaa agcgccagcg cgcctgaatg ccacaatcgc ttaatccgcg
    10861 ccctttcgct aaggtcacca gttcacgcga tttctcatca aatgttgcgt tcatcagttc
    10921 cagttcattc gtctcctgac cgttgttgaa cggcaaacgc aaccgaaccg ggcgcgaggc
    10981 tagcggcttt ttacgcgaca caatccatgc caaatcaatg gtgttatagg cccctgcctc
    11041 acagctaatc atcatcagtg ctttatcatc ggtcagcgca gtgacattca cctcacgacg
    11101 taacggatca agcgagcagc gcagaccatt catccgccag tttccataat ccagcaaatc
    11161 gttacgttct tcgagtgaga gtggcgtcgg cgttgggtta accaccgcga cctctttcag
    11221 cgcaggcgcg ggcggtacgc tgagaggcgg ttcgtcccct ttcttgatcc acgcggtttc
    11281 actgccaacg cgcttttgct gagcatcaat aaacaacaac gccgctttta agccgctcag
    11341 agaaatggtc tgattgccgt cgcgtaaggt gatcgccttc ccttcctgaa ttatctgcaa
    11401 aaacgcggtg atggttgccg tatcatcggt tactaataac catggtgaaa tccgccactt
    11461 gtcaccactt aatgccaacg gctcaccatc taatagcagc cgtggcgcta tctccccttc
    11521 agacgcatcc ggcgacttca acccaccgcg ctcaatacgt aaaacggcat cggtatgcgc
    11581 cccagcgctg cggctcaggg tcatcaccag tccattatga tcgcccgtat tgcgcgccac
    11641 acaaaaattt tgattattac aggtgacctg ccagtcggaa aacgcccgtt gcgctggtgc
    11701 cgcccacacc aaagacgtcg gcaacaaagc gaaaaaaaag agaaggaaaa tgcgatagcg
    11761 catggacggt acgaccccag aagttcaaac aaacagcaca agtcgtatct tcgttgcgca
    11821 ggtagcgcgg ctcaatcgga tttgtcggat tgagccaata ataaacaacg aaatattatt
    11881 gttatttttc agtccattaa atgtgacatt tgcaaatagt taagcaataa caccgtctta
    11941 ccgtcacgta tctcgccggt tttgatcatc tccagcgcct ggctgaacgg cagctcgagc
    12001 acttcaatat cttcatcttc aacaccgcca ccggcgttag cgcgctgatt gtcactgtat
    12061 tcggcgataa aaaagtggat tagctcagtc acaccgccag gcgacatata cagttcaaat
    12121 aatttgcgca cttcgcccac ttcatagccc gtctcttcaa tcgcttcttt acgaatgcac
    12181 acttccggtt cgtcgttatc cagcagcccg gcgcaggttt caatcagctg cccgctttca
    12241 ttgccattaa cccaggtagc gacacggaac tgacgaatca gaaccacgct ctttttcttc
    12301 gcgttgtaca ggaggatcgt cgcaccatta ccgcgatcgt aaacttcacg tttatggcgg
    12361 ataacttcgc catctttgcg cgtgagatcg taagtaatgt tgtgcagggt gaaatagtta
    12421 tcggagagaa ttttgtcttt aatgagggtg atttgttgcg tcataccgac tccacagcgc
    12481 gaaatgaaca attatattac gctgtgaagt cgggtttgtc tgcgcaggct atcagtgaga
    12541 tttgactgat tttaccccca gccagtcaat aatcccctgt gccgcatgac gtctttctgc
    12601 cattgcggta accaccagat ccgcaccacg cacggcgtcg ccaccggcga agattttcgg
    12661 attggtggtc tggtaacggt actggctttc cacatccgcg atgatgcggc cccatttgtc
    12721 taccgttaca ccgtgcgact ccagccacgg catcccgtgt ggattgaagc caaacgccat
    12781 aatcaccgca tcggctggca tgacaaattc actgccctca atcggtacag gtcgccgacg
    12841 cccctgggca tccggctcgc caagacgcgt gcgcaggaaa cgaattccgc aaacgtgacc
    12901 ttgctcattc agctcaagcg ccaccggctg gacgttaaat tcgaagttgg ccccctcttc
    12961 gcgggcgttc ttcacctctt tcttcgagcc tggcatgtta gcttcatcac gacgataagc
    13021 gcaggtgacg ttactcgcgc cgtggcgcag cgcagtacgc acacagtcca tcgcggtgtc
    13081 gccgccgccc agtaccacga cgttaagtcc ggcggtattg ataaacggct cttccggtag
    13141 ctcttcgagc cccatcacct gtttagtgtt ggcaatgagg aacggcagcg cgtcataaac
    13201 gcccggcgca tcttcattgg gtaaacccgc tttcatggaa cggtaagtgc ctacgccaac
    13261 gaagaccgcg tcgtattgtt ccaaaagcga atccaaagag acatctttac ccacttcaca
    13321 attgagttcg aagtgaatcc ccatcgcgct gaagatttcc cgacggcgtg ccagcaggga
    13381 tttatccagt ttgaaagaag gaatgccgaa agcgagcaag ccaccgattt ctggatggcg
    13441 atcgtacacc gtcaccgcca cgccattgcg ggtcagaaca tccgcacagg ccagccctgc
    13501 cggacctgcc ccgataatcg ccacccgctt gtccactttg gtgacatgac ttaagtcagg
    13561 acgccaacct ttcgccaacg cctgatctga aatatagcgt tcaatgttgc cgatagttac
    13621 cgcgccgtgc tcatcgcgaa tagtacaggc accttcacac aaacggtctt gcggacaaac
    13681 gcgtccggta atttccggca gggtgttggt ctggtgagaa agctcgacgg cggcgtcgat
    13741 gtttccggct ttcaccagtt caatccactg cggaatatgg ttatgcagcg ggcaggtcca
    13801 ttcacaaacg ctgtgctcgc cgaacttaag gcagcgcgag gcttcccgtt gtgcctggtc
    13861 ggcgcgaaat ggcagataaa tttcatcaaa accggttttg cgcgcttcaa tcgccagttt
    13921 atccggctcg ccacgcgcgg gcgttgcctg catttgttcg actttactca ttaccggcat
    13981 ttcttgtgca gcggtactgg catgccacgg ttgatgctcc tgacgtgcgg tgcgcaagcg
    14041 gcgggatttc gccatgccgg agagtgcgac gtcagtgacc agttgcagcg cgtccgccgg
    14101 gcaattctcg acacaggcag gaccgttttc gcgccctgca caaaggtcgc atttatgcgc
    14161 cgtggctttt acttttcctg ccgcgacggg tgtcaggacg atttgcatcg taccaaacgg
    14221 acaggccacc acgcaggatt tacagccaat acacttttgc tgattgacct gaatgctgtc
    14281 atcaacgtgg ctgattgcgc cattagggca gctacgggcg cagggcgcat cttcacaatg
    14341 gtgacaggtc actgcactac gttgctgctg atgtttgata accgtaattc ggggatgaaa
    14401 atggtgttgg ctcaggacat gttgctcatc attgtgagcc atgacacagg cgatttcaca
    14461 agcatgacaa cccagacatt gctgactgtt ggccataata aaacgattca taacgacctt
    14521 cttttttggt tgtaaaaacc ttattcttat atgagtgttg ttattacccg acttacaggg
    14581 gaaccggcaa tgttcatgtg cccagaataa gtaactattt cgctataaac tgtggcagat
    14641 caaataatcc catcactgac taaattgcgt ttcagcgaac tggaacatta atgatttttt
    14701 gtggagaaga cgcgtgtgat tgttaaacga cccgtctcgg ccagtctggc ccgggccttt
    14761 ttttacattg tgctgctgtc gattctttcc acgggtatcg ctctgctaac tctggcgagc
    14821 agtttgcgcg acgctgaggc tatcaatatt gccggatcgc tgcgtatgca gagttaccgc
    14881 ctgggctacg acttgcaaag tggcagtcca caactcaatg cacatcgcca gttgtttcag
    14941 caggcactgc attcaccggt attaaccaac ctcaacgtct ggtatgtgcc agaagcagta
    15001 aaaactcgct atgcgcatct gaatgccaac tggctggaga tgaataatcg gctcagcaag
    15061 ggcgatttgc cgtggtatca ggccaatatt aataattatg ttaatcagat agacctgttc
    15121 gtgctggctt tacagcacta tgctgaacgc aaaatgctgc tggtggtggc gatttccctg
    15181 gctggcggta tcggtatttt cacgctggtc ttttttactt tgcgccgcat acgccatcag
    15241 gtggttgccc cgctgaatca gctggttacc gccagtcagc gtattgaaca cgggcagttc
    15301 gactcgccgc cgctggatac cagtctgccg aatgagcttg gtctgcttgc aaaaaccttt
    15361 aaccaaatgt cgagcgagct gcataaattg tacctttcgc tggaggcgtc agtagaagaa
    15421 aagacccgcg atctccacga ggccaagcgt cgtctggagg tgttgtatca gtgttcccag
    15481 gcgctgaaca ccagccagat tgatgtgcat tgtttccgcc atattttgca gattgttcgc
    15541 gacaatgaag cagctgaata tctggagctg aacgttgggg ataactggcg gattagcgaa
    15601 gggaagccaa accctgagct gccaatgcag attttaccgg tgacaatgca agagacggtt
    15661 tacggcgaac tgcactggca aaatagtcac gtttcatcat cagaaccgct gcttaacagt
    15721 gtttcatcga tgctgggacg cggtttgtac tttaatcagg cgcagaagca ttttcagcag
    15781 ttattgttga tggaagaacg tgcgaccatc gcccgcgaat tgcacgactc gctggctcag
    15841 gtgctttctt acttacgtat ccagttgacg ttactgaagc gttcgatacc ggaagataac
    15901 gccactgcac aaagtatcat ggccgatttt tcccaggcgt tgaatgatgc ttatcggcag
    15961 ttacgcgagc tgttgactac tttccgcctg acgctgcagc aggcggatct cccctccgcg
    16021 ttgagggaaa tgctggatac gttacaaaat caaaccagcg ctaaactgac cctcgactgc
    16081 cgtctgccac ccttggcact ggatgcgcaa atgcaggtgc atttgttgca aattattcgc
    16141 gaagcggtgc tgaatgcgat gaagcacgcc aacgccagcg aaatcgccat cagttgcgtc
    16201 accgcgccgg acggcaatca cacggtttat atccgtgata acgggattgg tatcggtgaa
    16261 ccgaaagaac ccgaaggtca ttatggactg aatatcatgc gcgaacgcgc ggaacggctg
    16321 ggcgggacgc tgactttttc acaaccttcc ggcggcggca cgttagtgag tattagcttt
    16381 cgctccgcag agggggaggg gaaggggaac acgccggctg aggtgtaaat aattaccctc
    16441 acaccggccc tctcccttta aaggcgagag ggaaaaacgt gcccatattg acctgtaagc
    16501 gagtgtgtca gcaatctcat gataatttac attaactcct ttttttctcc acgattggct
    16561 cgtaccttgc cgctacagtg aagcaagtca agcctacaac aatacgcaga aacacgaggt
    16621 cctcttttaa tggcgaattt ctttattgat cgccccattt ttgcctgggt gctggcaatc
    16681 ctgttgtgtc tgacaggtac cctggcgatt ttttcattgc ccgttgaaca ataccccgat
    16741 ctcgcgccac cgaatgtgcg agtgaccgct aactatcccg gcgcatcggc ccagacgctg
    16801 gaaaacaccg tgacccaggt tatcgagcaa aatatgaccg gcctcgataa tctgatgtat
    16861 atgtcatctc agagcagtgg caccggtcag gcatctgtca ctttaagttt taaagcaggc
    16921 accgatccgg acgaagccgt gcagcaagta caaaaccagc tgcaatcagc catgcgaaag
    16981 ttaccgcagg cggtgcaaaa tcagggcgtg acggtgcgta aaaccggcga taccaacatt
    17041 ctgaccattg ccttcgtctc taccgatggc tcgatggata aacaggatat tgctgattat
    17101 gttgccagta atattcagga cccgttaagc cgcgtgaatg gcgtcgggga tatcgatgcc
    17161 tatggttcgc aatattccat gcgtatctgg ctggacccgg cgaaactcaa cagtttccag
    17221 atgacggcta aagatgtcac tgatgccatt gagtcacaga acgcgcagat tgcggttggg
    17281 caacttggtg gtacaccttc cgtcgataag caggcgctca acgccaccat taacgcccag
    17341 tcactgctgc aaacaccaga acagttccgc gatatcacct tgcgggtgaa tcaggacggc
    17401 tcagaggtaa ggctgggcga tgtcgccacc gtcgaaatgg gggcggagaa atacgattat
    17461 cttagccgct tcaatggtaa gccagcctcc gggttggggg taaaactggc ctccggcgca
    17521 aacgaaatgg cgacagcgga gctggtgctc aatcgtctcg acgagctggc gcagtatttc
    17581 ccgcacggac tggaatacaa ggtggcgtat gaaaccacct cgtttgttaa agcctccatt
    17641 gaagacgtgg tgaaaacgct gctggaagct atcgctctgg ttttcctcgt tatgtatctg
    17701 ttcctgcaaa acttccgcgc cacgctgata cccactatcg ccgtgccggt ggtgttgatg
    17761 ggaaccttct ccgtacttta cgccttcggt tacagcgtca acaccttaac catgttcgcg
    17821 atggtgctgg cgatcggtct gctggtggat gacgccatcg tggtggtgga aaacgtcgaa
    17881 cgtattatga gtgaggaagg actcactcct cgcgaagcca cacgtaaatc gatggggcag
    17941 atccaggggg cactggtcgg gattgcgatg gtgctttcgg cggtatttgt accaatggcc
    18001 ttcttcggcg gcaccaccgg tgccatctat cgccagttct ctattaccat tgttgcggcg
    18061 atggtgctgt cagtactggt agcgatgatc ctcactccgg ctctgtgtgc cacgctactt
    18121 aagccactga aaaaaggtga gcatcacggg caaaaaggtt tttttgcctg gtttaaccag
    18181 atgtttaacc gcaacgccga acgctacgaa aaaggggtgg cgaaaattct ccaccgtagc
    18241 ctgcgctgga ttgtgattta tgtcctgctg cttggcggca tggtgttcct gttcctgcgt
    18301 ttgccgacgt cgttcttacc gctggaagac cggggcatgt ttactacctc ggtacaattg
    18361 cccagcggtt caacccaaca acagaccctg aaagtcgttg agcaaatcga gaaatattac
    18421 ttcacccatg aaaaagacaa catcatgtcg gtgtttgcca ccgttggttc tggccctggg
    18481 ggtaacgggc aaaacgtggc gagaatgttt atccgcctga aagactggag cgaacgcgac
    18541 agtaagaccg gcacctcgtt tgccattatc gagcgtgcaa cgaaggcgtt taaccaaatt
    18601 aaagaagctc gcgttatcgc cagcagccca ccagcaatta gcggtcttgg tagttctgca
    18661 ggttttgata tggagttgca ggaccacgct ggagcgggtc acgatgcgct gatggcagca
    18721 cgtaatcagt tgctggcgct ggcggcggaa aacccggagc taacccgtgt gcgccataac
    18781 ggcctcgatg acagtccgca gttgcagatt gatatcgacc agcgtaaagc tcaggcgctg
    18841 ggcgttgata tcgacgatat taacgacaca ctgcaaaccg cctggggttc gagctatgtg
    18901 aatgacttta tggatcgcgg tcgcgtgaag aaagtctatg tgcaggcagc tgcgccgtat
    18961 cgcatgctgc cagatgacat caatctctgg tatgtccgaa ataaagatgg cggcatggtg
    19021 cccttctctg ctttcgcgac ctcacgctgg gaaacaggct cgccgcgtct ggaacgctat
    19081 aacggttatt ctgcggttga gattgttggg gaagccgcac cgggggtcag taccggtacg
    19141 gcgatggata ttatggaatc gttagtgaag cagctgccaa acggctttgg tctggagtgg
    19201 acggcgatgt cgtatcagga gcggctttcc ggcgcgcagg ctccggcgct gtacgccatt
    19261 tccttgctgg tggtattcct gtgtctggcg gcattgtatg aaagctggtc ggtaccgttc
    19321 tcggtaatgc tggtcgtgcc gctgggggta atcggcgcgc tgctggcaac ctggatgcgc
    19381 gggctggaaa acgacgttta cttccaggtg ggcctgttaa cggtcattgg tttatcggcg
    19441 aaaaacgcca tcctgatcgt cgagtttgct aacgagatga accaaaaagg ccacgacctg
    19501 tttgaagcga cgctccacgc ctgccgtcag cgtttacgcc cgattctgat gacctcgctg
    19561 gcatttatct tcggcgtatt gccaatggca accagcacgg gtgccggttc cggtggtcag
    19621 catgcggtgg gtactggcgt aatgggcggg atgatttcag ctactattct ggctatttac
    19681 ttcgtgccgc tgttctttgt gctggtgcgc cgccgcttcc cgctgaagcc gcgcccggaa
    19741 taagcaataa aaaaggcgac atgccaatgt gtcgcctttt tcaactttcc gattaagaac
    19801 ctgctcagcg ggttcttgct gtttatactt tgtctcagga attacttacg aagcataact
    19861 tcgataaagt ctttccagtt ccccagttca cgttcaatca taacaacctc tcttataatt
    19921 atgggtattc tacggaaaca atataccgtg gtgaagctaa tttactcgat tgctgcgatg
    19981 actacctccg ggggacaaac cttatgtaaa tactatggtc ctgcggtgat aatttgtatg
    20041 tgatacacag caacatttcg agatattcat acggcatcta atactgattt aattctggtt
    20101 aaaatacaga cagataacaa gatgaatatt cttaatgttt acgttaaaaa tgtttaatat
    20161 tatttaatag ttgttaattt gaatacttcg ataatgttat attttctgat aatcatttgc
    20221 aggcaaaatg ttttcaccct taaatgagta tttattctca taaatcgaaa aaggattcat
    20281 tatggttaca ctttacggca tcaaaaattg tgacaccatt aaaaaggctc gccgttggct
    20341 ggaagccaat aacatcgact atcgttttca tgattaccgc gtcgatgggc tggacagcga
    20401 attattgaac ggttttatca acgaattagg ctgggaagcg ttactcaaca cccgtggtac
    20461 aacctggcgt aaactggacg aaactacccg caataaaatc accgatgcgg cctctgcggc
    20521 ggcattaatg actgaaatgc ctgcaattat caaacgtcca ttgctctgcg cgcctggtaa
    20581 gcctatgctg ctgggtttca gtgattccag ttatcagcaa tttttccatg aggtgtagtc
    20641 tatgtcgtgc ccggttattg agctgacaca acagcttatt cgccgccctt ccctgagtcc
    20701 tgatgacgca ggatgtcagg ctttgttgat tgaacgtttg caggcgatcg gttttaccgt
    20761 tgaacgcatg gattttgccg atacgcagaa tttttgggca tggcgtgggc aaggtgaaac
    20821 gttagccttt gccgggcata ccgacgtggt gccgcctggc gacgccgatc gttggatcaa
    20881 tccgccgttt gaacccacca ttcgtgacgg catgttattc gggcgcggtg cggcagatat
    20941 gaaaggctcg ctggcggcga tggtggtagc agcagaacgt tttgtcgcac aacatcccaa
    21001 ccatacgggg cgactggcat ttctgatcac ttctgatgaa gaagccagtg cccacaacgg
    21061 tacggtaaaa gtcgtcgagg cgttaatggc acgtaatgag cgtctcgatt actgcctggt
    21121 cggcgaaccg tcgagtatcg aagtggtagg tgatgtggtg aaaaatggtc gtcgcggatc
    21181 gttaacctgc aacctcacca ttcatggcgt tcaggggcat gttgcctacc cacatctggc
    21241 tgacaatccg gtacatcgcg cagcaccttt ccttaatgaa ttagtggcta ttgagtggga
    21301 tcagggcaat gaattcttcc cggcgaccag tatgcagatt gccaatattc aggcgggaac
    21361 gggcagtaac aatgttattc cgggtgaact gtttgtgcag tttaacttcc gcttcagcac
    21421 cgaactgact gatgagatga tcaaagcgca ggtacttgcc ctgcttgaaa aacatcaact
    21481 gcgctatacg gtggattggt ggctttccgg gcagccattt ttgaccgcgc gcggtaaact
    21541 ggtggatgcg gtcgttaacg cggttgagca ctataatgaa attaaaccgc agctactgac
    21601 cacaggcgga acgtccgacg ggcgctttat tgctcgcatg ggggcgcagg tggtggaact
    21661 cgggccggtc aatgccacta ttcataaaat taatgaatgt gtgaacgctg ccgacctgca
    21721 gctacttgcc cgtatgtatc aacgtatcat ggaacagctc gtcgcgtgat gagtggttct
    21781 gcaagaggaa ataagcatgg actggctggc taaatattgg tggattctgg tgattgtctt
    21841 tttggtaggc gtcctgctga acgtgattaa agatctcaag cgcgtcgacc ataagaaatt
    21901 tctcgccaac aagccggagc ttcccccgca tcgtgatttc aacgataagt gggacgatga
    21961 cgacgactgg ccgaaaaagg atcaaccgaa gaagtaagct gagtgctcaa ccgtaggccg
    22021 gataaggcgt taacgccgca tccggcaatg gtgaacgatg cctgatgcga cgctcacgcg
    22081 tcttatcagg ccaggtaatc tgtcttttac atcatttcaa tcacatcatc atcgccaggc
    22141 ttaccgccac ttagcgcttc gtcgaaataa tgtttcggaa tggtataacg caagtgatcc
    22201 agggcaaatt gcatactgcg attgtcgatc gcatggccta aatcctccac gatatccagc
    22261 gtcacgtcac caccggcact gattaacgct tcctgcgccg caactgcatg cgccagatcg
    22321 ataaccggat cttcaccacc atgaatcaag tgaattgtcg tggctgtaga cgcggtctcc
    22381 ggcaggctcg catagcgtcc gttaaaggca atgacgcgcg aagcaaggcc cggttcggct
    22441 ttaatgctct ccagcgccat aatcgcgcct tgtgagaaac caattagcgc cgtggcgtta
    22501 gcccctactc cgctctgttt ctgccagtag cgtaccgttt caataaacgt cggcatgatg
    22561 gcatccacac gcgcctggcg attatcttcc gtaataccct gcactgaaaa ccactgacgc
    22621 cccgccggat taccgctcgg ctccgcgcca ccgacactca ccaccagcgc atcaggaaac
    22681 agcggtgcaa accagctgcc tatttccccc attgccaccg ggttatcccc gacaccgtgg
    22741 aaaagcagta acaactgttg tgcaggttta tccgggcttt gaacaacaaa atggtcatgt
    22801 ttcatggcga actccttaac tgatgtcatt aattttacgc cgctgactca caatgaccat
    22861 gggagaaaat tgaaggagtc agtgaaaaaa ttgccattgc gttatgcgat cgcgcagacg
    22921 ctcagtgcga acctcattaa gtgcgaacag cgcctgcgcg gcctcttccc gctggcgaac
    22981 cagtaacatc ttgcggcctg aaagtttaag tgtggtacat aactgcgcgt cactggcgtt
    23041 tttctgtaaa cgcccacgca gcgccggtaa tgccagttca ctggtttgta gcagacgcaa
    23101 taagcaacct aacgatgtta atagcggacg atgagcgaaa gcaaaaccgg ccagttcaag
    23161 ccagtcgtcg tcagaaagga tggcatcgtt tagtggatca acaggaagca tttcgccatt
    23221 ccactgcgcg agagcttgcg catcgcgacg taaacggtaa tgctcacgtt cagccagctg
    23281 tttacccgca tcactcatcg gtaacagcgc catcgccgta tagcaaccgc tgctggcttc
    23341 ccgatgatta cccatccgca ccagcacaaa accgcagcgt tgccagaaac gccataactc
    23401 cccggtgtaa ccaaaactca ccgaaagata gtcgaggtca tgcgtatatt gcaaagctcc
    23461 agcaataagt tgccgccctg tgccttcccg ctgacgagcc ggatgaactg ctatccggct
    23521 aacccgcctt ccgcgcaatg tcgccgccag tggattgctg ccgtgcgccg ccagcgactg
    23581 ggccaccaga ttaccccgcg ggcgacgaaa acctgcccat accgcctgac tgagttcttg
    23641 agataatccg ccctcatcca ccagccacaa cgccccggca atctcgtttt cgccagccgc
    23701 ctgtaaaaaa tgttgccctg gtgcatccat catccggcgt aaatccagcg gcgaggtccg
    23761 gtagtgcgca ccagacaata actgataaac ctttaacggc gtttctggct cgcttcgcca
    23821 taacgtttgt tcaaatgcgg aaatgacgat attgccttgt ggtgtatggg tgaagttttc
    23881 atcgttaaaa accagtgcct cactaaccat tttttccagc gggcatccct gtgcccagcg
    23941 gatcggctgt tgcagttcaa aacggtgtaa atgcggaaag cgagcgcaaa atttcagcaa
    24001 aaaaccacgt ccggtacctt cgtagccctg caccgtagtg gttaacaacg ttcgaggaaa
    24061 acgcgatacc agttgatgca acaacggcgc aggtatggct gcggcttcgt cgaccaccag
    24121 ccagtcggct tgctcatcgc tggctaacaa ggcatccggt gcaataaagc gaaacttctc
    24181 gcccgcaaat tgtgccagta catccgttgc cgcttttgcg ggcgcggtga caatcgcact
    24241 gcccgcaata cgagaaatga gttgccctgc caacgccgac ttaccgcgcc cacgcgcagc
    24301 cgttaccgct gccacgcccg gcggcatggt cagtagctgc tgtaagagtt gctgttgttc
    24361 tggttgtggt gcgccggtcg cggggtgcca gtcagtacgg ggagtaaaat gcgccaacga
    24421 gaacggctgg ttttgccgcc agaggatagc gtcgttatcc gccgtaagta cgcgtttgag
    24481 atgctggaca aaatgcggcg tcgcaatagg gtcagggcaa tcactccagc gcagcgagtc
    24541 ggcatcaggt tggttttccc actcttccca tacagggagt aacaaaacca gccagcttcc
    24601 cgctttcaac gttccgctaa gtgccgcaaa ggcagcggca tcaaagccgt ggcgggcgtc
    24661 gaataccgca tgccggaact cgcgcccaag taaagtttgt agcgccgagg gtgaacagtg
    24721 gttttcagca tctagctgcg gcgaaatcca cagccagtcg ccaggtaagg catcacgcaa
    24781 cttaagagca tgatcaaaac accaaccctc ttccccgctc aacaccagca agcggcagat
    24841 cccttcacgt ttcatttgtg ctgttaatgt gtaaagcgca gtcagttcag ccatccctgc
    24901 cccgaaaatt aaatgctttt accaaaagta ttgcattgtg ccggatcgcc gctgtcgaaa
    24961 ccacgtttaa accagctgta gcgttgctga gaagtgccat gtgtgaaact gtctggtact
    25021 actcgcccct gactttgctg ttgtaaacgg tcatcgccga tggcctgcgc cgcattcagc
    25081 gcctcttcca gatcgccagt ttccagaacg ccttgctgct gcatactatg cccccagaca
    25141 ccggcaaaac agtcggcctg gagttccata cgcacagata agtggtttac ttccgcctgc
    25201 gtcgcgtttt gttgcagttg acgaactttc ggctcgatgc ctaacagttt ctgcacatga
    25261 tgaccgactt catgggcgat aacgtacccc tgggcaaaat cgccatccgc gccaagtttg
    25321 tctttcatgt catcatagaa ggagagatcg atataaactg tgccatccgc cgggcaatag
    25381 aacggcccca taatggactg gcccgccccg cagccggtgc gcgtcattcc acggtacatg
    25441 accagcttcg gttgctgata ggtcttaccc atcttttcga actgttgtcc ccaggtgtct
    25501 tccgtggttg ccagaatcac cgaggtgaat tttgcggctt cgtcttcatt agggctaatt
    25561 gaccgcgttg attgttgttg agaaaccggc tgcccggtca tcaacccggt taaatcaacg
    25621 ccatagtagc ctgcaaccag cacgactatc agtaaaatca gcccgccttt accgctcggc
    25681 agacggaaac cgggaccgcc cattgacgga ccgccagagc tgttgcgcct gtcttcaaca
    25741 ttgtcacttt cacgtcgccc ttgccaacgc atagatacct caacaatata ttcattatag
    25801 aaatgatcgt aggtggttca gaggaagatt accacaggaa agcgggatga aatggcggag
    25861 gaatgagcgt tcaatgcata tattccgaga gcggttgtct tgccgtgcca gctgcacggc
    25921 aagatgatga acagaaaaat cagtccagct gtacacccag gcggcgggcg acggcttcat
    25981 aggcttcgat caggccaccg aggctctggc ggaaacggtc tttgtccatt ttctccagtg
    26041 tttctttgtc ccacaggcgg ctgccgtccg gggagaactc atcgcccagt accacttcac
    26101 ctttgtacag accaaactcc agcttgaagt cgaccagaat cagaccagca tcatcgaaca
    26161 gttttttcag cacgtcgttc gctttgtagg tcagctcttt catacgcgcc aggttctctt
    26221 tgctcaccca gccaaaggtt tcgcagtaag attcgttgac catcggatcg tgcatggcat
    26281 cgtttttcag gaacagatcg aacagcggcg ggttcagctc aataccttct tcgattccaa
    26341 gacgtttcac cagagagcca gcagcacggt tacgcacgac acactcaacc ggcaccatat
    26401 ccagcttttt caccagacat tcggtatcgg agagcagacg ctccatttga gtcgggatac
    26461 ccgcttcagc cagtttgctc ataatgaagt agttgaactt gttgttcacc atacctttgc
    26521 gatcaaactg ctcaatgcgc gcgccatccc ctgctgacgt atcattgcgg aattcgagca
    26581 ccaacaggtc cgggttttcc gtgctgtata cggttttcgc tttaccacga tacaactcag
    26641 cttgcttttg catctttatc actcctgggt gtgaattaac gttttaaaat cttttgctgt
    26701 ctggtgtgcc ggatgttttg tcggatgcgg cgtgaacgcc ttatccgaac tacgtccgac
    26761 aaacaaattt cgtgcgaatt accgctaaaa tcgcgttttc ctgccgacgc acacgtttgc
    26821 gtattatatc agaaaaaagg gccggatgat tccagccctg tatttttact tgctaaacgc
    26881 agcctggaag acagctacca gcgcgtcgtt ctgactctga gtcagagtat gacctttcgg
    26941 atcgatgaac tgtaggctgc tgcggttatc taaatcgcca acctgcagtt tatagtcacc
    27001 ggatgccagg cctggatcgc tcgcgcccag ttcctgccag tcgctgtcag acagcggctt
    27061 ataagttacg gccatgttgc cctgcgaacg ggtgctgtcg gtcactttca tgcccacttt
    27121 ttccagcgcc gctggcagac gttgccaaac cacattgaac ggcccgcgta cgaccagcat
    27181 tggtaaaccg gtgtcatcag ctgcactttg tacgtccata gtggtggagg cacgattttg
    27241 cgcagcgttc gcggcgtcag tggcagattt atccagaccg gcggaaataa cgttcatcat
    27301 ctccgtgctg taacgctgca tggaagccgc gtctgcaacc ggtttgcccg cctgttccag
    27361 gttcagcagt ttaaccgtaa ccgcctgctg ataaccctgc ggcttaacag agatttgata
    27421 acgaccacga tactgctcgt cttcgtccag acggttccat tgtacccaat cggtggtcag
    27481 tgtctgacca gcatcatcac gttgggtgat ggtgtagttt ttcgcctgca gcacgctaac
    27541 cacctgcggc cacagagtat tgccacgacc attttccacc agcaatgaag cggtatcgcc
    27601 cgtgaactgg gtacgcgcgc cagaaaccag tgccagcggc tgggctggtg gacgaatgtc
    27661 cagcgcctta ccgacagcac cactaccgtt ggtcaccggg attgcataat caccggaggt
    27721 caccggcaaa atcattccag ccggggcatg aagctccgca agcggtgccg cttccaggta
    27781 ggcttcatca ccactgacct gacgcttata gcgtgagtca gaactacagg cagcgagtaa
    27841 taaaacaagc gaaacacccg caacctttgc caggcgcgac ttttgaacag agtaagccat
    27901 caaatctccc taaactttac agcaaaccgg catgcttaag cgccgctctg accgtctcac
    27961 gaccactgtc ggtgattggt gtcattggca ggcgcagcgt atcggtcgcc acaagaccca
    28021 gttccttaca tgcccatttc accgggattg gattgggttc gacaaatagt ttgttgtgta
    28081 atggcatcaa acgctgatta ataacgcgtg cctcggcaaa atgcccttct gctgccagtt
    28141 tgcacatctg ggccatatca cgcgctgcga cgttagccgt aacggaaata accccatgac
    28201 cgcccaattg catgaagtcc agcgcgctcg catcatcgcc gctcagcaga acaaaatcat
    28261 ctgaaaccag ctctttgatc tggtttacac gcgttaagtt ccctgttgcc tctttgattc
    28321 cgataatatt ttttactttc gccagacggc ccaccgtttc cgggagcaga tcgcagccag
    28381 tacgggacgg cacattatac agaatttgcg gcaggtcagt atgctcagcg atggctttga
    28441 aatgctgata caaaccttct tgcgacggac gattgtagta aggagttacc gtcaggcagc
    28501 cgacgatacc actgacattg aagcgctgcg tcaggctaat ggcttccgca gtagcgttag
    28561 cgccggtccc ggcaattacc ggaatgcgcc catcagccag atccagcgtc atcatcacca
    28621 catcagcatg ttcgtcatga tttaaggtag cggactcgcc agtggtgcca acagaaacga
    28681 tcgccgaagt accgctggcg acatgataat caatcagttt tttcaagcta gcccgacaga
    28741 cattaccttt ttcatccatc ggagtaacaa tcgcgacaat acttcccgtg aacatgggcc
    28801 atcctctgtg caaacaagtg tctcaatggt acgtttggta tggcattaaa agcaagcaga
    28861 cagaaccgtt ctgattgttg tatgcatgat ttttttatgc tttccttaag aacaactcac
    28921 cccttaaagg aataaccagt ttgacactgt catcgcaaca ttatctggtg atcactgcgt
    28981 tgggtgccga tcgccctgga attgtgaaca ccatcacccg tcatgtcagt agttgcggct
    29041 gtaatattga agacagtcgc ctggcgatgc tgggagaaga gttcacgttt atcatgctgc
    29101 tttccggttc atggaatgcc atcactctga ttgaatcaac gttaccgttg aaaggtgccg
    29161 aactggatct tttaatcgtg atgaagagca cgacggcgcg tccgcgtccg ccaatgccag
    29221 catctgtctg ggttcaggtc gatgttgcag actccccgca tttaattgaa cgcttcacag
    29281 cacttttcga cgcgcatcat atgaacattg cggagctggt gtcgcgcaca caacctgctg
    29341 aaaatgaacg ggctgcgcag ttgcatattc agataaccgc ccacagcccc gcatctgcgg
    29401 acgcagcaaa tattgagcaa gcgttcaaag ccctatgtac agaactcaat gcacaaggca
    29461 gtattaacgt cgtcaattat tcccaacatg atgaacagga tggagttaag taatgaatcc
    29521 actgaaagcc ggtgatatcg caccgaaatt tagcttgccg gatcaagacg gagaacaagt
    29581 taatttgacc gacttccagg gacagcgtgt tctggtttat ttctacccga aagccatgac
    29641 ccccggctgt accgtacagg cctgcggctt acgcgataac atggatgagt tgaaaaaagc
    29701 gggcgttgat gtgctgggta tcagcaccga taaacccgaa aaactctccc gttttgcgga
    29761 aaaagagctg cttaacttta cgctcctgtc tgatgaggac caccaggtgt gcgaacaatt
    29821 cggcgtctgg ggtgaaaagt ccttcatggg caaaacctac gatggtattc atcgcatcag
    29881 cttcctgatt gacgctgatg gcaaaatcga acatgtcttt gacgatttca aaaccagcaa
    29941 tcaccacgac gttgtgctga actggctgaa agaacacgcc tgattacttt gctccattcc
    30001 gtgctggctg cgcttgcggc cagcatacct cacttctcgt gatcaagatc acattctcgc
    30061 tttcccctgc gacacgggtg tcgaatccat tttttgctga acgttaatga ccatcatttt
    30121 tgtaccgttc agaatccagt taatacataa cttattgaat agattgagtt aatcagaatg
    30181 gcatccttta tgcaatatga aatgcaatgt ttcatatcat tttcaaggag ccgacatgaa
    30241 ccgctttgtg gtggccgaac cactgtggtg tacaggatgt aatacctgtc tcgctgcctg
    30301 ttcggacgtg cataaaacgc aaggtttaca gcaacacccg cgcctggccc tggcgaagac
    30361 gtcaacaatc actgcccctg tcgtgtgtca tcactgtgag gaagcccctt gcctgcaggt
    30421 ctgcccggtc aatgccatct ctcagaggga tgatgcgatc caactcaacg aaagcctctg
    30481 tattggctgc aagctttgcg ccgtggtctg cccatttggc gcaatcagcg cttcaggaag
    30541 ccgtccggtg aatgcccatg cgcaatatgt ttttcaggct gaaggctcac tcaaagacgg
    30601 cgaagaaaac gtgccgcccc aacatgcgtt gctgcgctgg gaaccgggtg tccagaccgt
    30661 cgcggtgaaa tgcgacctgt gtgatttctt gccagaaggt ccggcctgcg ttcgcgcttg
    30721 cccgaatcag gcgttacggc tgatcaccga cgatagcctg caacgtcaga tgaaagaaaa
    30781 acagcgcctt gccgcaagct ggtttgccaa tggcggggag gatccccttt ccctcactca
    30841 ggagcaacac taatggatgc cctgcaatta ttaacctggt cgctgatcct ctatctgttt
    30901 gccagtctgg cttcgctgtt tttactcggt ctggacagac tggctattaa gctttccggc
    30961 atcacatcgc tggtgtgcgg cgtgattggc atcatcagcg gaattacgca attacatgct
    31021 ggtgtaactt tagtcgccca ttttgccacc ccttttgact ttgccgattt aaccctgcga
    31081 atggatagcc tctcggcatt tatggtgctg gttatctcct tgctggtggt ggtttgttcg
    31141 ctctattcat tgacttatat gcgcgaatac gagggcaaag gcgcggcggc gatgggcttc
    31201 tttatgaatc ttttcatcgc atcgatggtt gccctgctgg tgatggacaa cgctttttgg
    31261 ttcatcgtgc tgtttgaaat gatgtcgctg tcttcctggt ttctggtcat tgccaggcag
    31321 gataaaacgt cgatcaacgc tggcatgctc tactttttta tcgcccacgc cggatcggtg
    31381 ctgattatga tcgccttctt gctgatgggg cgcgaaagcg gcagcctcga ttttgccagt
    31441 ttcgcacgct ttcactttct ccggggctgg cgtcggcggt gtttctgctg gcctttttcg
    31501 gttttgccgc gaaagccggg atgatgccgt tgcacagctg gttgccgcgc gctcaccctg
    31561 ccgcaccatc gcacgcttcg gcgttgatgt ctggcgtaat ggtcaaaatc ggtattttcg
    31621 gcatcctgaa agtagcgatg gatctgctgg cgcaaacggg tttgcctctg tggtggggca
    31681 ttctggtgat ggcgatcggc gcaatctccg cgctcctggg cgtgctatat gcgctggcgg
    31741 aacaggatat caaacggctg ctggcctgga gtaccgtcga aaacgtcggc attattttgc
    31801 tggcagtcgg tgtggcgatg gacggtctgt cactgcacga cccgctgctc accgtggttg
    31861 gactgctcgg cgcactgttt catctgctca accatgcgct gttcaaaggg ctgctatttc
    31921 tcggcgcggg cgcgattatt tcgcgtttgc atacccacga catggaaaaa atgggggcac
    31981 tggcgaaacg gatgccgtgg acagccgcag catgcctgat tggttgcctc gcgatatcag
    32041 ccattcctcc gctgaatggt tttatcagcg aatggtacac ctggcagtcg ctgttctcac
    32101 taagtcgtgt ggaagccgta gcgctacaac ttgcgggtcc tattgctctg gtaatgctgg
    32161 cagtcactgg tgggctggca gtaatgtgct tcgtcaaaat gtacggtatt actttctgtg
    32221 gtgcgccgcg cagtacacac gctgaagagg cacaggaagt gccaaatacg atgatcgtcg
    32281 ccatgctact gctcgcggca ctctgcgtat taattgcgct tagtgccagt tggctggcac
    32341 cgaagataat gcatattgcc catgcgttta ccaatacccc tcccgtcact gtcgccagcg
    32401 gaatagcact tgtacccggc acgtttcata cacgggtcac tccttcatta ctgttgctgt
    32461 tactactggc gatgcctttg ctgcctggcc tttactggct gtggtgtcgt tcgcgccgcg
    32521 cagcgtttcg tcgcacagga gatgcctggg catgcggcta cggctgggaa aacgcgatgg
    32581 ctccgtcagg caatggcgtg atgcagccgc tgcgtgtggt cttctctgcg ttatttcgtc
    32641 tacgacaaca gctcgaccct acgctgaggc taaataaagg tcttgcgcac gtcaccgcca
    32701 gggctcagag cacagaaccc ttctgggatg agcgggtgat ccgccccatc gtgagcgcca
    32761 cccaacggct ggtcaaagaa atacagcatc tgcaaagcgg cgactttcgt ctctattgcc
    32821 tgtatgtggt cgccgcactg gttgtgctgc taatcgctat tgccgtctaa ggaaatcacc
    32881 atgagacaaa ctctttgcga cggatatctg gtcatttttg cgttagcaca ggccgtgatt
    32941 ctgctgatgc taaccccact ttttacgggt atttcccggc agatacgcgc gcgtatgcac
    33001 tcccgccgcg ggccggggat ctggcaggat tatcgcgata tccacaaact gtttaaacgc
    33061 caggaagttg cgccgacatc ttcaggtctg atgttccgcc tgatgccgtg ggtattaatc
    33121 agcagcatgc tggtgctggc gatggcctta ccactattta ttaccgtttc cccttttgcg
    33181 ggcggcggcg atctgatcac ccttatctat cttcttgccc tgtttcgttt tttctttgct
    33241 ctttccgggc tggataccgg aagtccgttt gcgggagtcg gtgccagtcg cgagttgacg
    33301 ctcggcattc tggtcgaacc aatgcttatt ctctcactgc tggtattggc gctgatagca
    33361 ggttccacgc atatcgagat gatcagcaat acgctggcga tgggctggaa ctcgccgcta
    33421 accaccgtac tggcgttact ggcctgtggt tttgcctgct tcattgagat gggaaaaatt
    33481 ccctttgatg ttgctgaagc agaacaggaa ttacaggaag gcccgctgac cgaatattcc
    33541 ggtgccgggc tggcgctagc gaaatggggg ctggggctga aacaggtcgt gatggcagca
    33601 ctgtttgtgg ccctgtttct gccctttggg cgcgcgcaag aactttctct cgcctgcctg
    33661 ctgacttcac ttgtcgttac gctgctcaag gttttgctga tttttgtact ggcctcaatc
    33721 gcagaaaaca cgctggcacg cgggcgtttt ttactcattc accatgtgac ctggcttggc
    33781 ttcagccttg ctgcgcttgc atgggtcttc tggttaaccg gtctgtaagg agcactgacg
    33841 gaatatggaa aatcttgctc tgacgacgtt aatgctgcct tttatcggcg cactggtcgt
    33901 ttcgttttcg ccacaacgtc gggccgccga atggggggtt ttgttcgccg cgctgaccac
    33961 gctgtgcatg ttgtcgctga tctccgcgtt ttatcaggcc gataaagttg ccgtcacgtt
    34021 gacgttggtc aacgtggggg atgtggcatt gtttggcctg gtcattgatc gcgtgagtac
    34081 gctgattctg tttgtggtgg tgtttctcgg tttgctggtc acgatctact ccacgggtta
    34141 tctgacggat aaaaatcgcg aacacccgca taacggcacg aatcgttatt acgcattttt
    34201 gctggtgttt atcggcgcga tggcgggact ggtactctcc tcgacgctgc tcggtcagtt
    34261 gttgtttttt gaaattacgg gcggctgctc ctgggcgttg atcagttatt accagagcga
    34321 taaagcgcag cgttcagcac taaaagcgtt gcttatcacc catatcggtt cgttggggtt
    34381 gtatcttgcc gccgccacgc tgtttttgca gaccggaacg tttgtgctta gcgcgatgag
    34441 cgagttacat ggcgacgcac gttatctggt ttatggcggc atcctgtttg ccgcgtgggg
    34501 gaaatcggcc cagctaccga tgcaagcgtg gctaccggac gcaatggaag cgccaacacc
    34561 gatcagcgcc tatctccacg ccgcatcgat ggtgaaagtg ggcgtttaca tttttgcccg
    34621 cgccattatc gacggcggca atatcccgca tgtgattggc ggcgttggca tggtcatggc
    34681 actggtcacc attctttatg gctttctgat gtatttgcca cagcaggata tgaagcggtt
    34741 gctggcctgg tcgaccatca ctcaacttgg ctggatgttc ttcggcttgt cgctctccat
    34801 cttcggctcg cggctggcgc tggagggtag catcgcctac atcgtcaacc acgcgttcgc
    34861 taaaagcctg tttttccttg tagcaggtgc gctgagttac agctgcggca cgcgcttgtt
    34921 gccgcgtctg cgtggcgtat tgcacaccct gccgttgcca ggcgtgggtt tctgcgtggc
    34981 agcgctggca attaccggtg tgccgccgtt caacggcttc ttcagtaaat tcccgctgtt
    35041 tgccgccggt tttgcgttgt cagtggagta ctggatcctg ctgcccgcca tgattctgct
    35101 gatgattgaa tcggtcgcca gtttcgcctg gtttattcgc tggttcggtc gcgtcgtgcc
    35161 gggcaaaccg agcgaggccg tcgccgatgc cgcaccgctg ctagggtcaa tgcgcctggt
    35221 gttgattgta ctgattgtga tgtcgctgat ttccagcgta atcgccgcga cctggttgca
    35281 gtaaggagat gatgaatgac cggttctatg atcgtaaata atctggcggg actgatgatg
    35341 ctgacatcgc tgtttgtgat tagcgtcaaa agctatcgcc tgtcatgcgg attttacgcc
    35401 tgccagtcgc tggtgctggt gtctattttc gccactctct cgtgcctgtt cgccgcagag
    35461 caactgctga tctggtccgc cagcgccttt atcaccaaag tgctgctggt accgttaatc
    35521 atgacttacg ctgcacgaaa tattccccag aacatcccgg aaaaagcgtt attcggtccg
    35581 gcaatgatgg cactgctcgc ggcgttaatt gtcctgcttt gcgcatttgt cgttcagccc
    35641 gtgaagctac cgatggctac cgggctgaaa ccggcgctgg cggtagcgtt aggtcatttt
    35701 ctgcttggcc tgctgtgcat tgtcagccag cgcaatatcc tgcggcaaat ttttggttac
    35761 tgcctgatgg aaaacggctc ccatctggtg ctggcgcttc ttgcctggcg agcaccggaa
    35821 ctggtggaaa taggtatcgc taccgacgcc atcttcgccg tcattgtgat ggtgttactg
    35881 gcaagaaaaa tatggcgtac ccacggcacg ctggacgtga acaacttgac cgcgctgaag
    35941 ggataatgag atgagttatt ctgtgatgtt cgctttactc ctgctcacgc cgctgctttt
    36001 ttcgctgctc tgttttgcct gccggaaacg ggggctctct gcgacttgta cggtgactgt
    36061 attacatagc ttagggatca cactgctgct gattctggca ctctgggtgg tccaaactgc
    36121 cgctgatgca ggagaaatat tcgctgcggg actgtggctt catattgatg gtctgggcgg
    36181 tttgttcctc gccattcttg gtgtgattgg ctttctcacc ggtgtttact cgattggcta
    36241 catgcttcat gaagtggcac gcggcgagct ttcacccgtt acactgtgcg attactacgg
    36301 tttcttccat ctgtttttgt tcaccatgct gctggttgtt accagcaata acctgattgt
    36361 gatgtgggcg gcgatcgaag ccaccacctt aagctcggcg tttctggtag gcatttacgg
    36421 tcagcgttca tcgctggaat cagcatggaa gtacatcatt atttgtactg ttggtgtcgc
    36481 ttttggtctg ttcggtaccg tgctggtata cgccaacgcc gccagcgtta tgccgcaggc
    36541 agaaatggcg atattctgga gcgaggttct taagcaatcg tccttgcttg acccaacatt
    36601 aatgctgttg gcctttgtgt ttgtgctaat tggctttggt accaaaaccg ggctatttcc
    36661 catgcacgcc tggctgccgg atgctcacag tgaagcgccg agtccggtca gcgccctgct
    36721 ctccgccgta ttgctgaact gcgcgctgtt ggtgctgatt cgctattaca tcattatttg
    36781 ccaggccatc ggcagcgatt tccccaaccg gttgttgctc atcttcggca tgttgtcggt
    36841 tgccgtggcg gcatttttca ttctggtaca gcgggacatt aagcgtctgc tggcgtactc
    36901 cagcgtggag aacatggggc tggtcgcggt ggcgctaggc attggcgggc cgctgggaat
    36961 ttttgccgcg ctgctgcaca ccttaaacca cagtctggca aaaacgctgc tgttctgcgg
    37021 ttccggcaat gtactgctca agtacggcac gcgcgatctc aacgtcgtct gtgggatact
    37081 caaaatcatg ccatttaccg ccgtgctgtt tggcggcggt gcgctggcgc tggcagggat
    37141 gccgcccttc aacatttttc ttagcgaatt tatgaccgtt accgcaggac tggcacgtaa
    37201 tcacctgctg attatcgtcc tgctgttatt gctgttaacg ctggtgctgg cgggcctggt
    37261 acggatggct gcgcgggtgt taatggcgaa accgccgcag gccgttaacc ggggtgatct
    37321 cggctggttg accacctcgc caatggtgat tctgctggtc atgatgctgg cgatgggaac
    37381 gcatattcca caacctgtca tcaggatcct ggcgggcgct tccactatag tcctctcagg
    37441 gacgcacgac ctgcctgcac aacgtagcac ctggcatgat tttttgcctt caggcaccgc
    37501 atctgtttcg gagaaacaca gtgaacgtta attcatcgtc aaatcgtggc gaagcgattc
    37561 tcgccgccct gaaaacgcag ttccccggcg cggtgctgga tgaagagcga caaacgcctg
    37621 aacaggtcac cattacggtg aaaatcaatc tgctgcctga cgttgtacat tatctttatt
    37681 atcaacatga tggctggctt ccagtcctgt ttggcaacga cgagcggaca cttaacggtc
    37741 attacgcggt ttattatgcc ctttcaatgg aaggggccga aaaatgctgg atcgtggtga
    37801 aggcactggt cgatgccgac agtcgggagt ttccgtcagt cacaccgcgc gtccctgccg
    37861 cggtctgggg cgagcgagaa attcgcgata tgtacgggct gattccggtt ggcctgccgg
    37921 atcagcgtcg cctggtgttg cccgatgact ggccggaaga tatgcatccg ctgcgcaaag
    37981 atgcgatgga ttatcgactg cgccctgaac cgacgacaga ttccgaaacg tatgcgttta
    38041 tcaatgaggg caacagcgat gcgcgggtga tccctgtcgg cccgctgcat atcacctcct
    38101 atgaaccggg tcacttccgc ttgtttgtgg atggcgagca aattgtcgat gctgattacc
    38161 gcctgtttta tgtccatcgc ggcatggaga aactggcaga aacgcggatg ggctacaacg
    38221 aagtgacctt cttatcggac cgcgtgtgtg ggatttgcgg ttttgcccac agtgtggcct
    38281 ataccaactc ggttgaaaat gcactgggga ttgaggtgcc gcaacgagca catactattc
    38341 gctcgattct gctggaagtc gagcagctac acagtcattt gctcaacctt ggcctctcct
    38401 gccattttgt tggttttgat accggcttta tgcaattttt ccgcgtgcgg gaaaagtcga
    38461 tgacgatggc ggaattgctg accgggtcgc gtaaaaccta cggtctgaat ctgattggtg
    38521 gtgttcgccg cgatattctc aaagagcaac gtctgcaaac gctgaaacta gtgcgcgaga
    38581 tgcgcgccga cgtgtcggag ctggtagaga tgctgcttgc tacgccgaat atggaacaac
    38641 gcactcaggg cattggcatt ctcgaccgac aaatcgcccg tgattatagc cctgtagggc
    38701 cgctgatccg cgcagtggtt ttgcccgtga tttgcgcttt gatcacccct acgccgacta
    38761 cggcaatatt ccaaaaacac tgtttacctt taccggcggc gatgttttct cccgcgtgat
    38821 ggtccgtgtc aaagagacgt ttgattcgct ggcaatgctg gaatttgccc tcgacaacat
    38881 gccggatact ccactgctga ccgaaggctt tagctataaa cctcacgcat tcgcgctggg
    38941 ctttgttgaa gcgccacgcg gtgaagacgt gcactggagc atgctcggtg ataaccaaaa
    39001 attgttccgc tggcgctgtc gtgccgccac ctacgccaac tggccggtgt tgcgttacat
    39061 gctgcgcggc aataccgttt ctgacgcacc gctgattatc ggtagccttg atccctgcta
    39121 ctcctgtacc gaccgtgtga cgctggtaga tgtgcgcaag cgccagtcaa aaaccgtgcc
    39181 gtataaagag atcgaacgct acggcattga tcgtaaccgt tcgccgctga agtaaggaca
    39241 gaagatgctg aagttactga aaactattat gcgcgccgga accacgacgg tgaaatatcc
    39301 cttcgcgcca ctggaggtca gcccagactt tcgcgggaaa ccggacctga tgcccagcca
    39361 atgtattgcc tgcggtgcct gcgcctgtgc ctgtgcttgt ccggcaaatg cgctgactat
    39421 ccagaccgac gaccagcaaa attcgcgcac ctggcagctc tatctggggc gttgtattta
    39481 ctgcggacgt tgtgaagaag tgtgcccgac cagagccatc cagcttacca ataactttga
    39541 actgaccgtc acccataaag ccgatctcta ttatacccac gcgacgttcc atctacaacg
    39601 ttgcagccgt tgcgaacgcc cgtttgcccc gcaaaaaacc gtcgcactgg ctgctgaatt
    39661 gttggcacag caacaaaatg cgccacaaaa ccgcgaaatg ttatgggcgc aagcgagcgt
    39721 ctgcccggag tgcaaacaac gcgcgacgct gatcaacgac gatacagatg taccgctggt
    39781 ggctaaggag cagctatgag tccagtgctt acgcaatatg tcagccagcc catcacgctg
    39841 gacgagcaaa cgcaaaagat gaagcggcat ttgctacagg atatccgtcg ctcggcttac
    39901 gtttatcgca tcgattgcgg cggctgcaac gcctgtgaaa tcgaaatttt tgctgccatc
    39961 acccccgtat tcgacgcaga acgttttggc attaaggttg tttcatcacc gcgtcacgcc
    40021 gatattttgt tatttactgg cgcagtcacc cgggcgatgc gtatgcctgc acttcgggcg
    40081 tatgagtctg cccccgatca taaaatctgt gtttcctacg gcgcgtgcgg tgtcggcggc
    40141 ggtattttcc acgatctcta cagcgtctgg ggcggcagcg acaccattgt ccccattgat
    40201 gtttggatcc ccggctgccc gccaacaccg gccgccacca ttcacggttt cgccgtggcg
    40261 ctcggtttgc tgcaacagaa gattcacgcg gtggattatc gcgatcccac cggggtgact
    40321 atgcaaccgt tgtggccgca gatcccgcca tcacagcgta tcgccattga gcgagaagcg
    40381 cggcggctgg cgggctatcg tcaggggcga gaaatttgcg atcggctcct gcgccattta
    40441 agcgacgatc ctacaggaaa tcgggttaac acctggttgc gcgatgccga cgatccacgt
    40501 ctcaatagta tcgttcagca actctttcgc gtactccggg ggttacatga ctgaagagtg
    40561 cggggaaatt gttttctgga cgctgcgaaa aaagtttgtc gccagtagcg acgagatgcc
    40621 ggaacacagc tctcaggtaa tgtattactc gctggctatc ggtcatcacg ttggcgtgat
    40681 tgattgtctg aatgtcgcct tccgctgccc actgacggaa tacgaagatt ggcttgcact
    40741 ggtcgaagag gagcaagccc gacgtaagat gctgggggtg atgacttttg gtgagattgt
    40801 tattgacgcc agccacaccg ccctgttgac ccgggcattc gcgccactgg cagatgacgc
    40861 gacgtctgtg tggcaagcgc gtagcattca attcattcat ctgttggatg aaattgtgct
    40921 ggaaccggct atctatctga tggccagaaa aattgcgtga gaaggatttc tcattaataa
    40981 ggactgttga tggctatgtc agacgaagca atgtttgccc cgccgcaagg aataacaatt
    41041 gaagcggtaa acggaatgct cgcggagcgg ttagcgcaga aacacggcaa ggcgtcttta
    41101 ttacgcgcct tcatcccgct gccgccgccg ttcagcccgg tacaacttat tgaactgcat
    41161 gttctcaaaa gcaacttcta ttaccgctac catgatgatg gcagcgatgt gacggcaaca
    41221 acagagtatc agggcgagat ggtcgattat tcgcgtcacg ccgtccttct cggcagtagt
    41281 ggaatggcgg agctacgctt tattcgtacc cacggcagcc gttttactcc ccaggattgc
    41341 acactgttta actggctggc tcgcattatc accccggttc tgcaatcatg gctcaatgat
    41401 gaagaacagc aggtggcgct gcgtttgctg gagaaagatc gcgatcatca tcgggtactg
    41461 gtggatatca ctaatgcagt gctgtcacat cttgatctcg acgatctgat cgctgacgtc
    41521 gctcgtgaga tccatcattt tttcggtctg gcttcagtca gtatggtact gggcgatcat
    41581 cgaaagaacg agaagttcag cctgtggtgc agcgatcttt ctgcctcaca ttgtgcgtgt
    41641 ctgccacgca atatgcctgg cgacagtgta ttgctgacac aaacgctaca aacccgacaa
    41701 ccgaccttga cgcaccgtgc agatgatctg tttctctggc aacgcgaccc gttattactc
    41761 ttacttgcat ctaacggctg cgaatctgcg ctccttatac cgcttacctt tggcaaccat
    41821 acaccgggtg cattgttgct ggcgcatacc tcttccactc tctttagtga ggaaaactgc
    41881 cagctactac aacacatagc cgatcgcatc gctattgccg ttggcaatgc cgatgcctgg
    41941 cgtagaatga ccgatttgca ggaaagtttg cagcaagaaa accaccagct tagcgagcag
    42001 ctcctttcga atctgggcgt cggtgacatt atctatcaaa gccaggcaat ggaagagctg
    42061 ctccagcagg tagatattgt ggcgaagagc gacagtacgg tgttgatttg cggtgaaacc
    42121 ggaaccggca aagaggtgat cgccagagcg atccatcaac ttagcccgcg acacgacaag
    42181 ccgctggtca aaatcaactg cactgccatc cccgccagtc ttctggaaag tgagttattc
    42241 ggtcatgaca aaggggcgtt tactggtgcg attaataccc atcgtggtcg ttttgaaatt
    42301 gccgatggcg gcacgttgtt tctcgatgaa attggcgatc tgccgttaga acttcagcct
    42361 aaactgctgc gcgtattgca ggaacgggag attgagcgtc tcggcgggag tagaacgatc
    42421 ccggtaaatg tcagagtcat tgccgccacc aaccgtgatt tgtggcaaat ggttgaagat
    42481 cgccagtttc gcagcgatct cttttatcgc ctgaatgtct tcccactgga attgcctccg
    42541 ctgcgcgacc gtccggaaga tatccctctt ttagcaaagc atttcacgca aaaaatggcg
    42601 cgccatatga atcgttcaat tgacgccatc ccgaccgagg cgctacgcca gttgatatcg
    42661 tgggactggc cgggcaacgt gcgcgagctg gaaaacgtga ttgagcgagc ggtactgctg
    42721 actcgcggta acagtctgaa tttacatctt aatgtccgac aaagccgttt actgccgacg
    42781 ctaaatgaag attcagcgct tcgcagttca atggcgcagt tgctgcaccc gacgacgcca
    42841 gagaatgacg aagaagaacg tcagcgcatt gttcaggtat tgcgagaaac caatggcatt
    42901 gttgccgggc accgtggcgc ggcgacacga ttagggatga agcgcaccac gctgctgtca
    42961 cgaatgcagc gtctgggaat ctcggttcgc gaggtgttgt aatctgcttt tgcaggagta
    43021 tgcatgagaa acaaactctc tttcgacttg cagttgagcg ccagaaaagc ggcaatcgct
    43081 gaacggattg ccgcccataa aattgcccgc agtaaagtgt cggtcttttt aatggcgatg
    43141 tccgctggcg tgtttatggc gatcggattt actttttacc tttccgttat cgccgatgcc
    43201 ccgtcttcac aggcattaac ccatctggtg ggcggccttt gctttacact tggctttatt
    43261 ttgctggcgg tttgcggcac cagcctgttc acctcgtcgg taatgacggt aatggcaaaa
    43321 agtcggggcg ttattagttg gcgaacttgg ctgattaacg cacttctggt ggcctgcggt
    43381 aatctggcag gtattgcctg tttcagtttg ttaatctggt tttccgggct ggtgatgagt
    43441 gaaaacgcga tgtggggagt cgcgatttta cactgcgccg agggcaaaat gcatcataca
    43501 tttactgaat ctgtcagcct cggcattatg tgcaatctga tggtttgcct ggcgctgtgg
    43561 atgagttatt gcggacgttc gttatgcgac aaaatcgtcg ccatgatttt gcccatcacc
    43621 ctgtttgtcg ccagtggctt tgagcactgt atcgccaatt tgtttgtgat tccgttcgcc
    43681 attgccattc gccatttcgc ccctacctcc ttctggcaac tggcgcacag tagcgcagac
    43741 aattttccgg cactgacggt cagccatttt attaccgcca atctgctccc ggtgatgctg
    43801 ggtaatatta tcggcggtgc agtgctggtg agtatttgtt atcgggctat ttatttacgt
    43861 caggaatcct gaaaaatcag cccggcgaaa cagttcgtcg ggctgaagat tattcttgcg
    43921 cgatttgccc atcgggccag gcgtggatca cggctttgat cagcgtcgcc aatggaatgg
    43981 cgaagaatac gccccagaat ccccacaaac caccgaagat caccaccgat aaaataatca
    44041 ccagcggatg caggttaacc gcttcggaga acaacaccgg tactagcagg ttgccgtcca
    44101 gcgcctgaat aatcagatac accgcgaagc agctccagaa ttcagtacct gcgccaaact
    44161 ggaatagcgc cacgccaacc accggaatgg tcaccacaaa tgcgccaatg tacggaataa
    44221 gaaccgagaa gccgaccagc actgccagca gcagcgaata gttcagcccg aagagcaaga
    44281 accccagcca ggtggcgatc cccaccacga tcatctccag cactttgccg cggatatagt
    44341 tggtgatttg ttgattcatc tccttccaca cctgtcctgc cagcccacgg ttacgcggca
    44401 gcacccggcg aacggcgttc agcatctgct ctttgtcttt cagcaggaag aagaccatca
    44461 atggcaccag caccagatag acggctatgg tcagcaaacc gaccagcgag gcgagggaaa
    44521 ttttcaccac cgaatcgccc atggtcagca tccgactgcg catattttcg gccattgcat
    44581 caataatgcc cgcgtccatt aacgccggat agcggcgcgg caacgtggcg gcaaagtcag
    44641 aaagcttatt gagcatcccc ggcatatcgc ggattaagta gatgccctgt tgccaggcga
    44701 taggcagtac cacgaacgcc atcagtagca atataccgac aaaaaccacc aatacaatcg
    44761 acgtcgccca gcggcgggag cagccaatag attgcaggcg cacggttggc cattccagca
    44821 aataagccag cacaatagcc accagcaacg gagcaagcag gccactaaag aaaaagataa
    44881 tgccaaatcc ggcaactaaa ataaccagca aggcaatcgc ttccgggtcg ctaaaacggc
    44941 ggcgatacca ttgcatcaac atttcgagca taaagacctt ccctgaacct caagagcggg
    45001 attgcgatcc gcaattgtat cgaaatgtca caaaaaagac ttcgcttttt atgacggatt
    45061 caggaaactg aaaagtcatt tgagtgggct aatcttcgcc gttacactca aaggcggcga
    45121 ggtgggaacg atatttcaca gtatcggtca aatgactatc ttcagaaata caggatagag
    45181 gttatgttca ggcagttgaa aaaaaacctg gttgcaaccc tcattgctgc tatgaccatt
    45241 ggtcaggtag ccccggcgtt tgccgacagc gcagacacct tgccggatat gggaacctcc
    45301 gcaggaagca cgctttccat tggtcaggaa atgcagatgg gcgactatta tgtccgccag
    45361 ctacgcggca gcgcgccgtt aattaatgac ccgctgttaa cgcaatatat taattcgctg
    45421 gggatgcgtc tggtttcgca tgccaattcg gttaagacac cgtttcattt ttttctgatc
    45481 aacaacgacg aaattaacgc ctttgctttc tttggcggca acgtggtgct gcactctgcc
    45541 ctgttccgtt attccgataa cgaaagtcaa ctggcttcag ttatggcgca cgaaatctcc
    45601 cacgtcaccc aacgtcacct ggcgcgagcg atggaagatc aacaacgcag cgcgccgctg
    45661 acctgggtcg gcgcgttagg ttctatttta ctggcgatgg ccagtccgca ggcggggatg
    45721 gcggcgctga ccggtacact ggcgggaacg cgccagggga tgatcagttt cacccagcaa
    45781 aatgaacagg aagcggaccg cattggtatt caggtgctgc aacgctcggg attcgatcca
    45841 caggcgatgc cgaccttcct ggaaaaattg ctcgatcagg cgcgttactc ctcgcgcccg
    45901 ccggaaattt tattgactca cccgttgccg gaaagtcgtc tggcagatgc ccgcaaccgt
    45961 gctaatcaga tgcgcccgat ggtggtgcag tcgtcggaag atttctatct ggcgaaagcg
    46021 cgcacactgg ggatgtataa ttccggacgt aaccagctca ccagtgattt gctggatgaa
    46081 tgggcgaaag gaaacgttcg tcagcaacga gcggcgcaat atggtcgtgc tttacaggcg
    46141 atggaagcca ataaatacga cgaggcgcgt aaaacgctgc aaccgttact ggcggcagaa
    46201 cctggcaacg catggtatct cgatctggct accgatatcg atcttgggca aaacaaagcc
    46261 aatgaggcaa tcaatcgcct gaaaaatgcc cgcgatttac gcaccaatcc ggtgttgcag
    46321 ctcaacctgg cgaacgctta tctgcaaggc ggtcaaccac aagaagcggc caatattctg
    46381 aatcgctaca cctttaataa taaagatgac agcaacggct gggatttgct ggcacaggcg
    46441 gaagccgcgc taaataaccg cgatcaggag ctggctgcgc gagcagaagg ttatgcgctc
    46501 gccggacgac tcgatcaggc catttcgctg ttgagtagcg ccagttcgca ggtgaaatta
    46561 ggcagcctgc aacaagcgcg ttacgatgcg cgcatcgacc agttgcgcca gctgcaggaa
    46621 cgctttaagc cttataccaa gatgtaattc gcattaaagg aagaacaatg accaaacagg
    46681 taaaaatcta ccataaccca cgctgttcaa agagccggga aacgctgaac ctgctgaaag
    46741 aaaacggcgt ggagccagaa gtggtgctct accttgagac acccgccgat gcggcaacgc
    46801 tgcgcgattt gctgaaaatg ctggggatga atagcgcccg tgaattgatg cgccagaaag
    46861 aggatcttta taaagaacta aacctggctg acagttcgct tagcgaagag gcgctgattc
    46921 aggcaatggt ggataatccg aagctgatgg agcgcccgat tgtggtcgcg aatggcaaag
    46981 cgcggattgg tcgtccaccg gagcaggtac tggaaatcgt gggttaagat gtgctccatc
    47041 gctagttgaa gcacattgcc ggatgcgacg cttgccacgt cttatccggc ctacgaatac
    47101 tatggattaa cttcgcgtag gtcaggcgtt cgcgccgcat ccgacaataa acaccttatc
    47161 tacaacttca gaatttcttt cacaaacgga atggtcagct tacgttgcgc ggtaatcgac
    47221 gcacgatcca actgatccaa cgtcataaat agcgtgcgca tttctctgtc gagccgcttc
    47281 agcaagaaac gccccacatc ttccggcagt tcaaaaccac gcaaacgcgc gcgtaactgt
    47341 agcgcctgca acttatcttc atcagaaagt ggctgcaatt tgtagatctg cccccagtcg
    47401 agacgcgacg cgagatccgg taatcccaga ttcaactgcc gcggtggacg atcgccggtg
    47461 atcaacaacc gtgttttgcc cgattccaga attcgattgt agagatcgaa aatcgccatc
    47521 tcccacaact catcgcctgc aatacactca atgttgtcga tacagaccag cgacaaatgc
    47581 tccataccgt cgagcacttc cggaacaaac caggtgcgtt tatccagcgg gacatagccc
    47641 accgcatcgc cacgctgcga caattccgcg caagccgcgt gcagcagatg gctgcgcccc
    47701 gcgccttcgc gtgcccagag atagatgtaa ccgctatgtt cctgacgcag cacgttttgc
    47761 agcgcggcca gtaaagagga gttatccccc ggccagaaac ttgcaaaggt ttcgtcgtca
    47821 ggaagataaa gtggcaaaga gagctgtgcc ggtgtgttca gagatacctc aaccaggatt
    47881 tcacaaaatc gcgagaagtt taccacagaa tcccatgatg tttgaaccgg gcagcaacac
    47941 tgcccggtcg gtacattatt tgtctgttat atccgcgtct tctgcgtcca gcaccacttc
    48001 ttcagggcgc agcacactga tcagtttgaa aatcaaactt aagccgatac cgacgatggt
    48061 cgccagcgcc atacctttta actccgccgc gccgatgttt accttcgcgc cactgacgcc
    48121 gatgatcaaa atcacggaag tcaggatcag gttctgtgct ttgttgtaat ccactttcga
    48181 ttcgatcaaa acacgaatac cggaagcacc aatgacacca taaagcagca gcgaaacgcc
    48241 gcccatcacc ggcaatggga tcatctggat agcggcagcc agtttaccga cgcaggaaag
    48301 caggatagcg aaaatcgccg ccccgccgat aacccaggta ctgtaaacac gggtgatcgc
    48361 catcacgcca atgttttctc cgtaagtggt atttggcgta gagccaaaga agccggagat
    48421 cacggtcgac aggccgttag caaacatcga acggtgcaga cctggatcgc gcagcagatc
    48481 ttttttgacg atattagccg ttactaccag gtgccctacg tgttcggcaa taaccactaa
    48541 cgccgctggc agaatagtca gaatggcaaa ccactcgaag cgcggcgtat agagggttgg
    48601 cagtgcaaac cagtgagcat taataatcgg cgtggtatcg acaattccca ttgcgaaaga
    48661 gagcgcgtac cccaccagca cgccaattaa aatcgggata attgccagga aaccacgaaa
    48721 cagcacggaa cctaaaaccg tgaccgccag ggtggtaata gagatgatga tggttttgga
    48781 gtttggcgtt tgcccttcag ccgggagtaa acccgccata ccggcagcta cgcccgccag
    48841 ctccagaccg atgacggcaa cgattgcgcc cattgccgca ggtggaaaca gcacgtccaa
    48901 ccagccggtc cccgctttct tcacgataaa agaaaccagg cagaacagca cgccgcacat
    48961 aataaagccg cccagcgcga cttcataccc taacggcaac agtaacaata ccggtgaaat
    49021 aaaggcaaag ctggaaccaa gataagccgg aattttccct ttacagatga agagatacag
    49081 cagcgttcca ataccgttaa ataacagtac agtcgccggg ttaatatgaa ataagacggg
    49141 caccaggacg gttgcaccaa acatggcgaa caaatgttgc aaactaagcg ggattgtctg
    49201 taaaagtggc ggtctttcac tcaccccgat agcacggcgc gtcatagtat tatcctctgt
    49261 attatgtgtt ataggcgctt tactcaaaaa aaagccgact cttaaagtcg gctttaatta
    49321 tttttattct ttatttcgta ccaaagattt tgtcaccggc atcgccgagg cccggaataa
    49381 tgtatccgtg ctcgttcagt ccctggtcaa tcgatgcagt atacagttcg acgtccgggt
    49441 gcgctttttc cagcgcagcg ataccttctg gcgcagcgac cagcaccaga actttgatgc
    49501 tgctgcagcc cgcttttttc agcaggtcga tggtcgcgat aacggaacca ccggttgcca
    49561 gcattgggtc aacgatcagc gccatacgct catcgatgtt agaaaccagt ttctggaagt
    49621 acggtaccgg ctccagcgtt tcttcattac ggtacatacc gacaacgctg atgcgcgcgc
    49681 tcggaacgtt ttccagcaca ccgtccatca taccaagacc cgcacgcaga attggcacaa
    49741 cggtaatttt cttacctttg atctggtcga tttctaccgg gccgttccag ccttcgatag
    49801 ttactttttc cgtttcgagg tcggcggtcg cttcgtaagt cagcaggcta cccacttcgg
    49861 aagcgagttc gcgaaagcgc ttggtgctga tatcttgctc acgcatcagt cccagcttgt
    49921 gtttgacgag tgggtgtttg acttccacga tcttcatact ctttctcctt tgaggggcag
    49981 ccacaaaaaa atcgacggat tatacctcct ttcttcaagg cggcaatatt attttcgttg
    50041 actttagtca aaatgataac agtttgagat aaagttattt tatattcaaa tggttatgaa
    50101 agaagattat tccatccgaa aactaacctt tgccctggca caagtcttct ttcgccgcgc
    50161 gcccggggaa aagacgtgca aaaaggttgt gtaaaacagt ctcgcaaacg tttgctttcc
    50221 ctgttagaat tgcgccgaat tttatttttc taccgcaagt aacgcgtggg gacccaagca
    50281 gtgaccgata aaacctctct tagctacaaa gatgccggtg ttgatattga cgcgggtaat
    50341 gctctggttg gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc
    50401 ggtctgggcg gcttcggtgc actgtgtgca ttgccgcaaa aatatcgtga acccgtgctg
    50461 gtttccggca ctgacggcgt aggtaccaag ctgcgtctgg caatggactt aaaatgtcac
    50521 gacaccattg gtattgatct ggtcgccatg tgcgttaatg acttggtggt gcaaggtgcg
    50581 gaaccgctgt ttttcctcga ctattacgca accggaaaac tggatgttga taccgcttca
    50641 gcggtgatca gcggcatcgc ggaaggttgt ctgcaatcgg gctgttctct ggtgggtggc
    50701 gaaacggcag aaatgccggg gatgtatcac ggtgaagatt acgatgtcgc gggtttctgc
    50761 gtgggcgtgg tagaaaaatc agaaatcatc gacggctcta aagtcagcga cggcgatgtg
    50821 ctgattgcac tcggttccag cggtccgcac tcgaacggct attcgctggt gcgcaaaatt
    50881 cttgaagtca gcggttgtga tccgcaaacc accgaacttg atggtaagcc attagccgat
    50941 catctgctgg caccgacccg catttacgtg aagtcagtgc tggagttgat tgaaaaggtc
    51001 gatgtgcatg ccattgcgca cctgaccggc ggcggcttct gggaaaacat tccgcgcgta
    51061 ttgccagata atacccaggc agtgattgat gaatcttcct ggcagtggcc ggaagtgttc
    51121 aactggctgc aaacggcagg taacgttgag cgccatgaaa tgtatcgcac cttcaactgc
    51181 ggcgtcggga tgattatcgc cctgcctgct ccggaagtgg acaaagccct cgccctgcta
    51241 aatgccaacg gtgaaaacgc gtggaaaatc ggtatcatca aagcctctga ttccgaacaa
    51301 cgcgtggtta tcgaataatg aatattgtgg tgcttatttc cggcaacgga agtaatttac
    51361 aggcaattat tgacgcctgt aaaaccaaca aaattaaagg caccgtacgg gcagttttca
    51421 gcaataaggc cgacgcgttc ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata
    51481 cgctcatcgc cagcgcgttt gacagtcgtg aagcctatga ccgggagttg attcatgaaa
    51541 tcgacatgta cgcacccgat gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg
    51601 cgtttgtctc ccactatgcc gggcgtttgc tgaacattca cccttctctg ctgccgaaat
    51661 atcccggatt acacacccat cgtcaggcgc tggaaaatgg cgatgaagag cacggtacat
    51721 cggtgcattt cgtcaccgat gaactggacg gtggcccggt tattttacag gcgaaagtcc
    51781 cggtatttgc tggtgatacg gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg
    51841 ctatttatcc actggtgatt agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg
    51901 ccgcgtggct ggatggtcaa cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc
    51961 cccgtagtta aagcgccagc tctgccgctg gcgtttttca attcacctgt aaatcgcaag
    52021 ctccagcagt ttttttcccc cttttctggc atagttggac atctgccaat attgctcgcc
    52081 ataatatcca ggcagtgtcc cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa
    52141 agctatacat cgaaaaagag ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag
    52201 cggcggacaa atctaacccg ctgattgaaa ggatgcgttt cctggggatc tattccaata
    52261 accttgatga gttctataaa gtccgcttcg ctgaactgaa gcgacgcatc attattagcg
    52321 aagaacaagg ctccaacgct cattcccgcc atttactggg caaaattcag tcccgagtgc
    52381 tgaaagccga tcaggaattc gacggcctct acaacgagct attgctggag atggcgcgca
    52441 accagatctt cctgattaat gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc
    52501 attattttaa gcagtatctg cgtcagcaca ttacgccgat tttaatcaat cctgacactg
    52561 acttagtgca gttcctgaaa gatgattaca cctatctggc ggtggaaatt atccgtagcg
    52621 atagcatccg ttacgcgctg ctggagatcc catcagataa agtgccgcgc tttgtgaatt
    52681 taccgccaga agcgccgcgt cgacgcaagc cgatgattct tctggataac attctgcgtt
    52741 actgccttga tgatattttc aaaggcttct ttgattatga cgcgctgaat gcctattcaa
    52801 tgaagatgac ccgcgatgcc gaatacgatt tagtgcatga gatggaagcc agcctgatgg
    52861 agttgatgtc ttccagtctc aagcagcgtt taactgctga gccggtgcgt tttgtttatc
    52921 agcgcgatat gcccaatgcg ctggttgaag tgttacgcga aaaactgact atttcccgct
    52981 acgactccat cgtccccggc ggtcgttatc ataattttaa agactttatt aatttcccca
    53041 atgtcggcaa agccaatctg gtgaacaaac cactgccgcg tttacgccat atttggtttg
    53101 ataaagccca gttccgcaat ggttttgatg ccattcgcga acgcgatgtg ttgctctatt
    53161 atccttatca cacctttgag catgtgctgg aactgctgcg tcaggcttcg ttcgacccga
    53221 gcgtactggc gattaaaatt aacatttacc gcgtggcgaa agattcacgc atcatcgact
    53281 cgatgatcca cgccgcacat aacggtaaga aagtcactgt ggtggttgag ttacaggcgc
    53341 gtttcgacga agaagccaac attcactggg cgaagcgcct gaccgaagca ggcgtgcacg
    53401 ttatcttctc tgcgccgggg ctgaaaattc acgccaaact gttcctgatt tcacgtaaag
    53461 aaaacggtga agtggtccgt tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg
    53521 cgcgtcttta tactgactat tcgttgctga ccgcagatgc gcgtatcacc aacgaagtac
    53581 ggcgggtatt taactttatt gaaaacccat accgcccggt gacatttgat tatttaatgg
    53641 tgtcaccgca aaactctcgc cgtctgttat atgaaatggt agaccgcgaa atcgccaacg
    53701 cgcagcaagg gctgcccagt ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc
    53761 tggttgatcg tctgtatgcg gcctccagct ccggcgtacc ggttaatctg ctggttcgcg
    53821 gaatgtgttc gctgatcccc aatctggaag gcattagcga caacattcgt gccatcagta
    53881 ttgttgaccg ttaccttgaa catgaccggg tttatatttt tgaaaatggc ggcgataaaa
    53941 aggtctacct ttcttccgcc gactggatga cgcgcaatat tgattatcgt attgaagtgg
    54001 cgacaccgct gctcgatccg cgcctgaagc agcgggtgct ggacatcatc gacatattgt
    54061 tcagcgatac ggtcaaagca cgttatatcg ataaagaact cagtaatcgc tacgttcccc
    54121 gcggcaatcg ccgcaaagta cgggcgcagt tggcgattta cgactacatc aaatcactcg
    54181 aacaacctga ataaccctat gccaatacac gataaatccc ctcgtccgca ggagtttgct
    54241 gcggtcgatc ttggctcaaa cagttttcac atggtcatag cccgtgtggt agatggtgcc
    54301 atgcagatta ttggccgcct gaaacagcgg gtgcatctgg cggacggcct ggggccagat
    54361 aatatgttga gtgaagatgc aatgacgcgc ggtttgaact gtctgtcgct gtttgccgaa
    54421 cggctacaag ggttttctcc tgccagcgtc tgtatagtcg gcacccatac gctgcgtcag
    54481 gcgctgaacg ccactgactt tctgaaacgc gcggaaaagg tcattcccta cccgattgaa
    54541 attatttccg gtaatgaaga agcccgtctg atttttatgg gcgtggaaca tacccaaccg
    54601 gaaaaaggtc gcaaactggt tattgatatt ggcggcggat ctacggaact ggtgattggt
    54661 gaaaatttcg aacctattct cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag
    54721 ctttattttc ctggcggggt catcaataaa gagaattttc agcgcgctcg catggcggca
    54781 gcacaaaaac tggaaacttt aacctggcaa ttccgtattc agggctggaa cgttgcaatg
    54841 ggcgcttccg gtaccataaa agccgcccat gaagtgttaa tggaaatggg cgagaaagac
    54901 gggataatta ccccggaacg tctggaaaaa ctggtaaaag aagttttacg tcaccgtaat
    54961 ttcgcatcgc tgagtttacc gggtctttcc gaagagcgga aaacagtctt cgttcctgga
    55021 ctggcgattt tatgtggtgt gtttgatgct ttagccatcc gtgaactgcg cctttctgac
    55081 ggggcgttac gcgaaggcgt actgtatgaa atggaaggac gtttccgtca tcaggatgtg
    55141 cgtagtcgca ccgccagcag cctcgccaac cagtatcaca tagacagcga acaggcccga
    55201 cgagtgctgg ataccactat gcaaatgtac gaacagtggc gggaacagca accgaagctg
    55261 gcgcatccgc aactggaggc gctactgcga tgggccgcca tgctgcatga ggtcgggttg
    55321 aatatcaacc acagcggttt gcatcgccac tccgcttata ttctgcaaaa cagtgacttg
    55381 ccgggtttta atcaggaaca gcagctgatg atggcgacac tggtgcgcta tcaccgtaaa
    55441 gcgattaagc tcgacgatct gccgcgcttt accttgttta agaagaaaca gttcctgcca
    55501 ctgatacagc tattgcgcct tggcgtatta ctaaacaatc aacgtcaggc aaccaccaca
    55561 ccgccaacat tgacactgat tactgatgac agtcactgga cactgcgttt cccgcatgac
    55621 tggtttagtc agaatgcgct ggtactgctt gatctggaaa aggagcaaga atactgggaa
    55681 ggcgtggctg gctggcggtt gaaaattgaa gaagaaagta ccccagaaat cgccgcttaa
    55741 tgctttgcgg gccgacgaga aatgtcggcc cgcattattc aggcactttc gcgaatgggt
    55801 tcgatttcat tcagcgtatc aattaacggt tgcggcttac caataagata accctgcata
    55861 taatcgatcc ccaaagagag caccgcctcg cggatctctt cgttttcaac gtactctgcc
    55921 actacccgca ttttcttcat tcgcgccaga tggcaaatcg acgccacaat ctgataatcc
    55981 agactatttg acacaatatt gcggataaag ctgccgtcaa ttttaagcag atcggcattc
    56041 acatttttaa gccgtgcata gctggcgtag ccggtgccga aatcatcaat cgcaatctgg
    56101 cagcctaatt cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca
    56161 ttactttcgg tgacttcaaa aataagttgc cacgcttcaa tctgatattt agccagcaac
    56221 tgactgactt caacaggaaa acgagcctga cataccgagg ttggagacag attaatagca
    56281 aaacggtgag cgggcatctt cgctctgttt tcagccataa attgcagcgt atgctcaatg
    56341 acccacatgt cgatactcga cgataaacca aattcgtgcg cgaccggcaa gaagctatcg
    56401 gggccgatca gttcatcatt ctcacctttc atgcgcagaa gaatttcatg gtaaacatca
    56461 ccacgcatac cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc
    56521 tgtagtcgat tcatcatcgc gactttatct ttcaattcgc gttgcaaata cattgccccg
    56581 caacgctgca tattttccgg ggcgttggtc acgatggaaa gttcggcgac cgtatttagc
    56641 tctcccagca gcaggtagat atgattcact ggcgagcgca catagcagta actgacgcca
    56701 atctgcggtt gcatcggcat cccatcccaa aagaaacgaa attgcttgag atgactatcc
    56761 agtgcggtaa tgcgctcctg gtgcgattct gtattcagtc gcagcgcgag atcgttaccc
    56821 gaaagctgat aaacatcttc acccggttcc agcaagggtg acagccagtg agaaagtttt
    56881 tgcttgtatt gaatccgcag catgatgcca tagttcttaa ccagcatttc catgccaagg
    56941 atgcgtaaat aacaaagcgc agaccaaggg gcatcacgca acgcgcgatt cagggcgcga
    57001 acatttggca gatgaaccac cgggtccaca tacgcaagcc gctgaatgcg tctgactacc
    57061 gctcgctgac gagttgccag tacagccata taattgacaa taaaagagaa taccagataa
    57121 ctggaggagg ttatggtcag ctgcgtggta tagccaggat aaatgggaat gtaattttga
    57181 tagctgtgga tgctgatcat caacacgacc gcccagagca gcgaaatcag cttataacca
    57241 tagcgcattg ctccccacat catcaggggc agcaataatg acaaggtata attggtgcta
    57301 aaaattgtgc ttttttcatt taacggcatg cataacagca acaataaagc acctaatgcc
    57361 agtagccaga gcgcgaactc ttttttggtg actttggcat caacctgctg ttttaattgc
    57421 gaataatagc tacgtaaata aaatggattt cgcactaccc gaatgatgaa gtagcacagc
    57481 gggacaccga tcagattacc caccagtaag gcctgataat tgattaaagt cccgaggtta
    57541 aaaggcatga caccgaccag attttctctg ctcgccagta atcctacaaa tgcagcaaac
    57601 tggaaaagta tcagaaacag cgttgcagga aacactatct gccagaatat acgctgggaa
    57661 attaaacggg tatcaccatg tgaaacgttg ttacgccggg gagtaaagac cctgtaaccg
    57721 ccccaacaga gaatgataat gataataaaa ttagccgtta atgagagcgt ttcgtaaaaa
    57781 cccagctctg catatttgcg gacaaaaatc cctaaggtga tccctggcaa tgccgcccag
    57841 ctgaaaaaca tcatcatgct gatcatcagt gccaaaggca gataaaagag aaaaacctca
    57901 ccggaaaaaa tatgcgcgaa agtattaatg tgggcgaaaa tgggcaagat taaagaaggc
    57961 aggaacagcg gaagccccca ccatttatca cgtattttta tataagttgc attcagtttc
    58021 atagatgctc agcagaatcc cccacatcct gaaggaggtg tattcagaca ggcatcccac
    58081 ctgacttcga atgatgatta ttcatcacta tagagagcat tgattctaag tgtcatatga
    58141 aagttccaat tgatatatat caaacaaaat aaccctgatt aatgaattat tacgtttatc
    58201 atgttaattc atcattatta catcatcatt gtaaataatt aaattaactt ccataacatt
    58261 aaaatatgta tccactgacg cttttttaca taacgaagaa ttgaccattt tgtcctgttg
    58321 tgccttaatg taagtaccgt ccacagcgtg ggacatactt caaggaacct tttgtgagtc
    58381 aggcaactag tatgcgaaaa cgacaccgat ttaacagtcg catgacccgt atcgtactgc
    58441 tcatcagctt tatcttcttc tttggccgtt ttatctactc gtccgtcggt gcctggcagc
    58501 accatcagag caaaaaagaa gctcagcaat ccacactctc cgtcgaatca ccggtacaac
    58561 gttagcggtt accttctcca ctttcacaga acataacggc acttcgctgt cggatgcttt
    58621 tgctctttgg gattatcaaa gcggcagata ttctttcatc ttaaatttta cgtctttgtc
    58681 ctgactgatg tttatcctgt ttggctgcga aataaatata aaattaatat atatgttgta
    58741 atgatatatt tttataaatt attccctgcg tgaattttaa taaatttaat ctatcccttt
    58801 atacgcaata catttacttt cctcttttga tgatcttaaa tgtcttattt ttcgtaatgt
    58861 gtataacaag gaatagtgat gaaatttaaa aaatgtcttc tgcctgtggc aatgttagcg
    58921 tcattcactc tggcaggatg ccagtcaaat gctgacgatc atgccgccga tgtttatcaa
    58981 accgatcaac tgaataccaa acaagaaact aaaaccgtta atattatttc cattcttccc
    59041 gcaaaagttg ccgtagacaa ctcccaaaat aaacggaacg cacaagcctt cggcgcgctt
    59101 attggcgcag tcgctggcgg tgttatcggc cacaacgtcg gttctggcag caattccgga
    59161 acgacggcag gggcagttgg cggcggagct gtaggcgcgg cagcgggttc tatggtgaat
    59221 gataaaacct tagtggaagg tgtttcttta acatataa
//
LOCUS       DBKEKI010000009        56536 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.009, whole genome shotgun sequence.
ACCESSION   DBKEKI010000009 DBKEKI010000000
VERSION     DBKEKI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.009"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..461)
                     /locus_tag="ACUQPX_000532"
     CDS             complement(<1..461)
                     /locus_tag="ACUQPX_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HHV9055329.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFR"
     gene            633..1286
                     /gene="rluE"
                     /locus_tag="ACUQPX_000533"
     CDS             633..1286
                     /gene="rluE"
                     /locus_tag="ACUQPX_000533"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HHV9055330.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTKRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            1296..1757
                     /gene="nudJ"
                     /locus_tag="ACUQPX_000534"
     CDS             1296..1757
                     /gene="nudJ"
                     /locus_tag="ACUQPX_000534"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HHV9055331.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            1811..2917
                     /gene="mnmA"
                     /locus_tag="ACUQPX_000535"
                     /gene_synonym="trmU"
     CDS             1811..2917
                     /gene="mnmA"
                     /locus_tag="ACUQPX_000535"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HHV9055332.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGSIIEQRLPLPV"
     gene            2953..3594
                     /gene="hflD"
                     /locus_tag="ACUQPX_000536"
     CDS             2953..3594
                     /gene="hflD"
                     /locus_tag="ACUQPX_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HHV9055333.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            3598..4968
                     /gene="purB"
                     /locus_tag="ACUQPX_000537"
     CDS             3598..4968
                     /gene="purB"
                     /locus_tag="ACUQPX_000537"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HHV9055334.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5137..5808
                     /gene="phoP"
                     /locus_tag="ACUQPX_000538"
     CDS             5137..5808
                     /gene="phoP"
                     /locus_tag="ACUQPX_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HHV9055335.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            5808..7268
                     /gene="phoQ"
                     /locus_tag="ACUQPX_000539"
     CDS             5808..7268
                     /gene="phoQ"
                     /locus_tag="ACUQPX_000539"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HHV9055336.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAEESMLGGARMEVIFGRQHSAPKDE"
     gene            7344..8465
                     /gene="roxA"
                     /locus_tag="ACUQPX_000540"
                     /gene_synonym="ycfD"
     CDS             7344..8465
                     /gene="roxA"
                     /locus_tag="ACUQPX_000540"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HHV9055337.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(8514..9740)
                     /gene="pepT"
                     /locus_tag="ACUQPX_000541"
     CDS             complement(8514..9740)
                     /gene="pepT"
                     /locus_tag="ACUQPX_000541"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HHV9055338.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEALSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            9990..11126
                     /gene="potA"
                     /locus_tag="ACUQPX_000542"
     CDS             9990..11126
                     /gene="potA"
                     /locus_tag="ACUQPX_000542"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HHV9055339.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11110..11967
                     /gene="potB"
                     /locus_tag="ACUQPX_000543"
     CDS             11110..11967
                     /gene="potB"
                     /locus_tag="ACUQPX_000543"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HHV9055340.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            11964..12758
                     /gene="potC"
                     /locus_tag="ACUQPX_000544"
     CDS             11964..12758
                     /gene="potC"
                     /locus_tag="ACUQPX_000544"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HHV9055341.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            12755..13801
                     /gene="potD"
                     /locus_tag="ACUQPX_000545"
     CDS             12755..13801
                     /gene="potD"
                     /locus_tag="ACUQPX_000545"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HHV9055342.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(13957..14778)
                     /gene="cobB"
                     /locus_tag="ACUQPX_000546"
     CDS             complement(13957..14778)
                     /gene="cobB"
                     /locus_tag="ACUQPX_000546"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HHV9055343.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(14794..15705)
                     /gene="nagK"
                     /locus_tag="ACUQPX_000547"
     CDS             complement(14794..15705)
                     /gene="nagK"
                     /locus_tag="ACUQPX_000547"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HHV9055344.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(15734..16978)
                     /gene="lolE"
                     /locus_tag="ACUQPX_000548"
     CDS             complement(15734..16978)
                     /gene="lolE"
                     /locus_tag="ACUQPX_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HHV9055345.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(16978..17679)
                     /gene="lolD"
                     /locus_tag="ACUQPX_000549"
     CDS             complement(16978..17679)
                     /gene="lolD"
                     /locus_tag="ACUQPX_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HHV9055346.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(17672..18871)
                     /gene="lolC"
                     /locus_tag="ACUQPX_000550"
     CDS             complement(17672..18871)
                     /gene="lolC"
                     /locus_tag="ACUQPX_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HHV9055347.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            19133..20206
                     /locus_tag="ACUQPX_000551"
     CDS             19133..20206
                     /locus_tag="ACUQPX_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HHV9055348.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            20334..23780
                     /gene="mfd"
                     /locus_tag="ACUQPX_000552"
     CDS             20334..23780
                     /gene="mfd"
                     /locus_tag="ACUQPX_000552"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HHV9055349.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMVHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            23923..24885
                     /gene="ldtC"
                     /locus_tag="ACUQPX_000553"
     CDS             23923..24885
                     /gene="ldtC"
                     /locus_tag="ACUQPX_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HHV9055350.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPIGHHAIRLAAYDGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(24968..25225)
                     /gene="bhsA"
                     /locus_tag="ACUQPX_000554"
     CDS             complement(24968..25225)
                     /gene="bhsA"
                     /locus_tag="ACUQPX_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA];
                     GO_process: GO:0046688 - response to copper ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HHV9055351.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            25467..26099
                     /gene="comR"
                     /locus_tag="ACUQPX_000555"
     CDS             25467..26099
                     /gene="comR"
                     /locus_tag="ACUQPX_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HHV9055352.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(26161..26700)
                     /locus_tag="ACUQPX_000556"
     CDS             complement(26161..26700)
                     /locus_tag="ACUQPX_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HHV9055353.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(26927..28231)
                     /gene="ndh"
                     /locus_tag="ACUQPX_000557"
     CDS             complement(26927..28231)
                     /gene="ndh"
                     /locus_tag="ACUQPX_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HHV9055354.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(28639..29181)
                     /gene="ycfP"
                     /locus_tag="ACUQPX_000558"
     CDS             complement(28639..29181)
                     /gene="ycfP"
                     /locus_tag="ACUQPX_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HHV9055355.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(29204..30229)
                     /gene="nagZ"
                     /locus_tag="ACUQPX_000559"
     CDS             complement(29204..30229)
                     /gene="nagZ"
                     /locus_tag="ACUQPX_000559"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HHV9055356.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(30240..31064)
                     /gene="thiK"
                     /locus_tag="ACUQPX_000560"
     CDS             complement(30240..31064)
                     /gene="thiK"
                     /locus_tag="ACUQPX_000560"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HHV9055357.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(31045..31686)
                     /gene="lpoB"
                     /locus_tag="ACUQPX_000561"
     CDS             complement(31045..31686)
                     /gene="lpoB"
                     /locus_tag="ACUQPX_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HHV9055358.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQLGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(31701..32078)
                     /locus_tag="ACUQPX_000562"
     CDS             complement(31701..32078)
                     /locus_tag="ACUQPX_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HHV9055359.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(32081..32440)
                     /gene="hinT"
                     /locus_tag="ACUQPX_000563"
     CDS             complement(32081..32440)
                     /gene="hinT"
                     /locus_tag="ACUQPX_000563"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HHV9055360.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            32762..34951
                     /gene="fhuE"
                     /locus_tag="ACUQPX_000564"
     CDS             32762..34951
                     /gene="fhuE"
                     /locus_tag="ACUQPX_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HHV9055361.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDHVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(35011..36444)
                     /gene="ptsG"
                     /locus_tag="ACUQPX_000565"
     CDS             complement(35011..36444)
                     /gene="ptsG"
                     /locus_tag="ACUQPX_000565"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0055056 - D-glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - D-glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HHV9055362.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(36739..37536)
                     /locus_tag="ACUQPX_000566"
     CDS             complement(36739..37536)
                     /locus_tag="ACUQPX_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HHV9055363.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(37547..38551)
                     /gene="holB"
                     /locus_tag="ACUQPX_000567"
     CDS             complement(37547..38551)
                     /gene="holB"
                     /locus_tag="ACUQPX_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HHV9055364.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(38548..39189)
                     /gene="tmk"
                     /locus_tag="ACUQPX_000568"
     CDS             complement(38548..39189)
                     /gene="tmk"
                     /locus_tag="ACUQPX_000568"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HHV9055365.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(39179..40201)
                     /gene="yceG"
                     /locus_tag="ACUQPX_000569"
     CDS             complement(39179..40201)
                     /gene="yceG"
                     /locus_tag="ACUQPX_000569"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HHV9055366.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLGTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(40204..41013)
                     /gene="pabC"
                     /locus_tag="ACUQPX_000570"
     CDS             complement(40204..41013)
                     /gene="pabC"
                     /locus_tag="ACUQPX_000570"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HHV9055367.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISSDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(41133..42374)
                     /gene="fabF"
                     /locus_tag="ACUQPX_000571"
     CDS             complement(41133..42374)
                     /gene="fabF"
                     /locus_tag="ACUQPX_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HHV9055368.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(42461..42697)
                     /gene="acpP"
                     /locus_tag="ACUQPX_000572"
     CDS             complement(42461..42697)
                     /gene="acpP"
                     /locus_tag="ACUQPX_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HHV9055369.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(42908..43642)
                     /gene="fabG"
                     /locus_tag="ACUQPX_000573"
     CDS             complement(42908..43642)
                     /gene="fabG"
                     /locus_tag="ACUQPX_000573"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HHV9055370.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(43655..44584)
                     /gene="fabD"
                     /locus_tag="ACUQPX_000574"
     CDS             complement(43655..44584)
                     /gene="fabD"
                     /locus_tag="ACUQPX_000574"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HHV9055371.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALLLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(44600..45553)
                     /gene="fabH"
                     /locus_tag="ACUQPX_000575"
     CDS             complement(44600..45553)
                     /gene="fabH"
                     /locus_tag="ACUQPX_000575"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HHV9055372.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(45621..46691)
                     /gene="plsX"
                     /locus_tag="ACUQPX_000576"
     CDS             complement(45621..46691)
                     /gene="plsX"
                     /locus_tag="ACUQPX_000576"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HHV9055373.1"
                     /translation="MKRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(46772..46945)
                     /gene="rpmF"
                     /locus_tag="ACUQPX_000577"
     CDS             complement(46772..46945)
                     /gene="rpmF"
                     /locus_tag="ACUQPX_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HHV9055374.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(46997..47518)
                     /gene="yceD"
                     /locus_tag="ACUQPX_000578"
     CDS             complement(46997..47518)
                     /gene="yceD"
                     /locus_tag="ACUQPX_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HHV9055375.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            47717..48301
                     /gene="yceF"
                     /locus_tag="ACUQPX_000579"
     CDS             47717..48301
                     /gene="yceF"
                     /locus_tag="ACUQPX_000579"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HHV9055376.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(48413..49372)
                     /gene="rluC"
                     /locus_tag="ACUQPX_000580"
     CDS             complement(48413..49372)
                     /gene="rluC"
                     /locus_tag="ACUQPX_000580"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HHV9055377.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            49945..53130
                     /gene="rne"
                     /locus_tag="ACUQPX_000581"
     CDS             49945..53130
                     /gene="rne"
                     /locus_tag="ACUQPX_000581"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HHV9055378.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(53327..54280)
                     /gene="flgL"
                     /locus_tag="ACUQPX_000582"
     CDS             complement(53327..54280)
                     /gene="flgL"
                     /locus_tag="ACUQPX_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HHV9055379.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAVALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(54292..55934)
                     /gene="flgK"
                     /locus_tag="ACUQPX_000583"
                     /pseudo
     CDS             complement(54292..55934)
                     /gene="flgK"
                     /locus_tag="ACUQPX_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(56000..>56536)
                     /gene="flgJ"
                     /locus_tag="ACUQPX_000584"
     CDS             complement(56000..>56536)
                     /gene="flgJ"
                     /locus_tag="ACUQPX_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HHV9055380.1"
                     /translation="AVPRNYDDSLPGDSRAFLAQLSLPAQLASQQSGVPHHLILAQAA
                     LESGWGQRQIRHENGEPSYNLFGVKASGNWKGQVTEITTTEYENGEAKKVKAKFRVYS
                     SYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAGYATDPHYARKLTNMIQQMK
                     SISDKVSKTYSMNIDNLF"
BASE COUNT        13813 a        15044 c        14100 g        13579 t
ORIGIN      
        1 tcacggaaga taaccatatc ggtcagttca gggtgtttaa ccgggcttgg agtgccctga
       61 tagtaacgta ccggacgcag gcagatgtag agatccagtt cctgacgcag ggcaacgttg
      121 agagaacgaa taccgccacc aaccggagtg gtcagcgggc ctttaatggc aacgcgatat
      181 tcacgaatca gatcaagggt ttcagcaggc agccagacat cctgaccata aacctgtgtg
      241 gatttttcac cggtgtaaat ttccatccag gagattttac gctcgccttt ataggctttc
      301 tcgactgcag cgtcgaccac tttcagcatg gctggggtta catctacacc gattccatca
      361 ccttcaatgt aagggataat cggattttca ggaacgttga gtttgccgtt ttgcagggtg
      421 atcttcttgc cttgtgccgg aacaactact ttactttcca ttcacctctc cttcgagcgc
      481 tactggtttg ctcgtctgcc accacgttgc atatgcgttt gcgtcctgcg atacggatgc
      541 tttagagcaa ttttttgtta atgatttgta attgacttgt caatactacc ctattgtttg
      601 ccgtcatgaa agatgcgcgt tattaggcta taatgcggca attcataatc tctgaaaata
      661 ccatgcaaaa aacttctttt agaaatcacc aggttaagcg attcagctcg caacgttcta
      721 ccaagcgtaa acatgaaaac cagcccacgc gtgtgatcct cttcaataaa ccctacgatg
      781 ttcttccgca gttcaccgat gaagccggac gcaaaacatt aaaagaattc atcccggttc
      841 agggcgttta tgcagcaggt cgccttgacc gcgatagcga agggttgctg gtgctgacca
      901 ataacggcgc gttgcaggcg cgtttaaccc agccgggtaa acgcaccgga aaaatctatt
      961 atgtgcaggt ggaaggtatt cccacacaag acgcacttga agccttgcgc aatggcgtaa
     1021 ccttaaatga tggccctacc ctgcccgcag gcgcggagct ggttgacgaa cccgcgtggt
     1081 tatggccgcg gaatccacca attcgtgaac gcaaaagtat tcccaccagc tggctgaaga
     1141 tcaccttata tgaaggacgt aatcgccagg tgcgccgcat gaccgcccat gttggtttcc
     1201 ccacgctgcg actgattcgc tatgcgatgg gagattactc tttggataat cttgccaatg
     1261 gcgaatggcg agaagtgaca gattaaggat gcaaaatgtt taaaccgcac gttaccgttg
     1321 cttgcgtggt gcacgcagaa ggcaaatttt tagtcgttga agagacgatt aatggtaaag
     1381 cgttatggaa ccaacctgcc gggcatctgg aagccgatga aaccttagtg gaagccgccg
     1441 cccgtgaact gtgggaagaa accggcatca gcgcgcagcc gcaacacttt attcgtatgc
     1501 atcagtggat tgcgccagat aaaacgccgt ttttgcgttt cctctttgcc attgagcttg
     1561 agcaaatatg cccgacccag cctcatgaca gcgatatcga ctgctgccgt tgggtcagcg
     1621 ccgaagaaat tttacaggcg tcaaatcttc gttcgccgct ggtggcggaa agtattcgtt
     1681 gttatcaaag cgggcaacgt tatccgctgg agatgattgg cgattttaac tggcctttta
     1741 caaagggtgt catctaaaag gctgcgtgat agaatacgcc gccttgaagt tcaatgtcgt
     1801 gagtgatcca atgtctgaaa ccgcaaaaaa agtaatcgtc ggcatgtccg gcggtgtcga
     1861 ttcctccgtt tctgcctggc tgttgcaaca acagggatat caggtcgaag gcctgtttat
     1921 gaagaactgg gaagaagacg acggtgagga atattgcaca gcggcagcgg atctggctga
     1981 tgcccaggcg gtctgcgaca agctcggcat tgaactgcac accgttaact ttgctgccga
     2041 gtactgggac aacgtcttcg aactgttcct tgccgaatat aaagccggtc gcacgccgaa
     2101 tccggatatt ctgtgcaaca aggagatcaa atttaaagcc ttcctcgaat ttgccgccga
     2161 agatttaggt gccgattata tcgctaccgg tcattacgta cgtcgggccg atgtcgatgg
     2221 caagagccgc ctgctgcgtg gtctggacag caataaagac cagagctact tcctttatac
     2281 gctcagccat gagcagattg cgcaaagcct gttcccggtc ggcgaactgg aaaaaccgca
     2341 ggtgcgtaag attgctgaag atcttggtct ggtcaccgcg aagaaaaaag actctaccgg
     2401 catttgcttc attggcgaac gtaaattccg cgagttcctg ggccgttatc tcccggcgca
     2461 accgggcaaa atcattaccg tcgatggcga tgaaattggt gagcaccagg ggctgatgta
     2521 tcacactctc ggtcagcgta aaggtctggg tatcggtggc accaaagaag gcaccgaaga
     2581 accgtggtat gtggtggaca aagacgtcga aaacaacatt ctggttgtcg ctcagggcca
     2641 tgaacacccg cggctgatgt ctgtcgggtt gattgcccag cagctgcact gggtcgatcg
     2701 cgaaccattc accggcacta tgcgttgcac ggtaaaaacc cgctatcgcc agaccgacat
     2761 tccgtgcacc gtcaaggcgc tggacgacga tcgcattaaa gtgattttcg atgagccggt
     2821 tgccgccgtg acgccgggcc agtctgccgt cttctataac ggtgaagtgt gcctcggtgg
     2881 cagtattatt gagcagcgtc tgccgctgcc ggtctgatta ttatctttac ttaaaacaag
     2941 gaagcagtga acgtggcaaa gaattactat gacatcaccc tcgccctggc cggtatttgt
     3001 cagtcggcac gcctggtgca acaactcgct caccaggggc attgtgatgc cgatgcgcta
     3061 cacgtctcac tcaacagtat tattgatatg aaccccagct cgacgctggc ggtttttggc
     3121 ggtagcgaag ccaacctgcg cgtcgggctg gaaaccctgc tcggcgtgct caatgccagc
     3181 agttgccagg gcttaaacgc cgaattaacc cgctacacac tcagcttgat ggtgcttgag
     3241 cgcaaactct cctcagcgaa aggcgcgctc gacactctgg gcaaccggat caacggcctg
     3301 caacgccagc tcgaacactt cgatttacag tccgaaacgc tgatgagcgc gatggctgct
     3361 atctatgttg atgtgatcag cccgcttggc ccgcgcattc aggtcaccgg ttcccctgct
     3421 gtactgcaaa gcccacaagt gcaggcgaaa gttcgcgcaa ccctgctggc aggcattcgc
     3481 gccgccgtgc tctggcacca ggtcggcggc ggacgtctac aactgatgtt ttctcgtaat
     3541 cgcctgacca ctcaggcaaa acaaattctt gctcatttaa ccccggagtt gtgatctatg
     3601 gaattatcct cactgaccgc cgtttcccct gtcgatggac gctacggcga taaagtcagc
     3661 gcgctgcgcg ggattttcag cgaatatggt ttgctgaaat tccgtgtaca agttgaagta
     3721 cgttggctgc aaaaactggc cgcgcacgca gcgatcaagg aagttcctgc ttttgctgcc
     3781 gacgcaatcg gttaccttga tgcaattgtc gccaatttca gcgaagaaga tgccgcacgc
     3841 atcaaaacca tcgagcgtac cactaaccac gacgttaaag cggttgagta tttcctgaaa
     3901 gaaaaagtgg cggagatccc ggaactgcac gcggtttctg aattcatcca ctttgcctgt
     3961 acttcggaag atatcaataa cctctcccac gcattaatgc tgaaaaccgc gcgtgatgaa
     4021 gtgatcctgc cgtactggcg tcaactgatt gatggcatta aagatctcgc cgttcagtac
     4081 cgcgatatcc cgctgctgtc tcgtacccac ggtcagccag ccacgccgtc aaccatcggt
     4141 aaagagatgg caaacgtcgc ctaccgtatg gaacgccagc accgccagct taaccaggtg
     4201 gagatcctcg gcaaaatcaa cggcgcggtc ggtaactata acgcccacat cgccgcttac
     4261 ccggaagttg actggcatca gttcagcgaa gagttcgtca cctcgctggg tattcaatgg
     4321 aacccgtaca ctacccagat tgaaccgcac gactacattg ccgaactgtt tgattgcgtt
     4381 gcgcgcttca acaccattct gatcgacttt gaccgtgacg tctggggtta tatcgccctt
     4441 aaccacttca aacagaaaac cattgctggt gagattggtt cttccaccat gccgcataaa
     4501 gttaacccga tcgacttcga aaactccgaa gggaatctgg gcctttccaa cgcggtattg
     4561 cagcatctgg caagcaaact gccggtttcc cgctggcagc gtgacctgac cgactccacc
     4621 gtgctgcgta acctcggcgt gggtatcggt tatgcgctga ttgcgtatca atccaccctg
     4681 aaaggcgtga gcaaactgga agtgaaccgt gaccatctgc tggatgagct ggatcacaac
     4741 tgggaagtgc tggcagaacc aatccagaca gttatgcgtc gctatggcat cgaaaaaccg
     4801 tacgagaagc tgaaagagct tactcgcggt aagcgcgttg acgccgaagg catgaagcag
     4861 tttatcgatg gtctggcgtt gccagaagaa gagaaagccc gcctgaaagc gatgacgccg
     4921 gctaactata ttggtcgagc tatcacgatg gttgatgagc tgaaataagc ctcaaatcag
     4981 tgccggatgg cgatgctgtc cggcctgctt attaagatta tccacttttt cttttttcac
     5041 tttacctccc ctccccgctg gtttatttaa tgtttacccc cataaacaca taatcgcgtt
     5101 acactatttt aataattaag acagggagaa ataaaaatgc gcgtactggt tgttgaagac
     5161 aatgcgttgt tacgtcacca ccttaaagtt cagattcagg atgctggtca tcaggtcgat
     5221 gacgcagaag atgccaaaga agccgattat tatctcaatg aacatttacc ggatattgcg
     5281 attgtcgatc tcggattgcc tgacgaggac ggtctgtcac tgattcgccg ctggcgtagc
     5341 aacgatgttt cactgccgat tctggtatta accgcccgtg aaagctggca ggacaaagtc
     5401 gaagtattaa gtgccggtgc tgatgattac gtgactaaac cgtttcatat tgaagaggtg
     5461 atggcgcgaa tgcaggcatt aatgcggcgt aatagcggtc tggcttcaca ggtcatttcg
     5521 ctccccccgt ttcaggttga tctctctcgc cgtgaattat ctattaatga cgaagtgatc
     5581 aaactgaccg cgttcgaata caccattatg gaaacgttga tacgcaataa tggcaaagtg
     5641 gtcagcaaag attcgttaat gctacaactc tatccggatg cggagctgcg ggaaagccat
     5701 accattgatg tactgatggg acgtctgcgc aaaaaaattc aggcacaata tccgcaagaa
     5761 gtcattacca ccgttcgcgg ccagggctac ctgttcgaat tgcgctgatg aaaaaattac
     5821 tgcgtctttt tttcccgctc tcgctgcggg tacgttttct gttggcaacg gcagcggtag
     5881 tactggtgct ttcgcttgcc tacggaatgg tcgcgctgat cggttatagc gtcagtttcg
     5941 ataaaactac gtttcggctg ttacgtggcg agagcaatct gttctatacc cttgcgaagt
     6001 gggaaaacaa taagttgcat gtcgagttac ccgaaaatat cgacaagcaa agccccacca
     6061 tgacgctaat ttatgatgag aacgggcagc ttttatgggc gcaacgtgac gtgccctggc
     6121 tgatgaaaat gatccagcct gactggctga aatcgaatgg ttttcatgaa attgaagcgg
     6181 atgttaacga taccagcctc ttgctgagtg gagatcattc gatacagcaa cagttgcagg
     6241 aagtgcggga agatgatgac gacgcggaga tgacccactc ggtggcggta aacgtctacc
     6301 cggcaacatc gcggatgcca aaattaacca ttgtggtggt ggataccatt ccggtggagc
     6361 taaaaagttc ctatatggtc tggagctggt ttatctatgt gctctcagcc aatctgctgt
     6421 tagtgatccc gctgctgtgg gtcgccgcct ggtggagttt acgccccatc gaagccctgg
     6481 caaaagaagt ccgcgaactg gaagaacata accgcgaatt gctcaatcca gccacaacgc
     6541 gagaactgac cagtctggta cgaaacctga accgattgtt aaaaagtgaa cgcgaacgtt
     6601 acgacaaata ccgtacgacg ctcaccgacc tgacccatag tctgaaaacg ccactggcgg
     6661 tgctgcaaag tacgctgcgt tctctgcgta gtgaaaagat gagcgtcagt gatgctgagc
     6721 cggtaatgct ggagcaaatc agccgcattt cacagcaaat tggctactac ctgcatcgtg
     6781 ccagtatgcg cggcgggaca ttgctcagcc gcgagctgca tccggtcgcc ccactgctgg
     6841 acaatctcac ctcagcgctg aacaaagtgt atcaacgcaa aggggtcaat atctctctcg
     6901 atatttcgcc agagatcagc tttgtcggtg agcagaacga ttttgtcgag gtgatgggca
     6961 acgtgctgga taatgcctgt aaatattgcc tcgagtttgt cgaaatttct gcaaggcaaa
     7021 ccgacgagca tctctatatt gtggtcgagg atgatggccc cggtattcca ttaagcaagc
     7081 gagaggtcat tttcgaccgt ggtcaacggg ttgatacttt acgccctggg caaggtgtag
     7141 ggctggcggt agcccgcgaa atcaccgagc aatatgaggg taaaatcgtc gccgaagaga
     7201 gcatgctggg cggtgcgcgg atggaggtga tttttggtcg ccagcattct gcgccgaaag
     7261 atgaataaat atgtccatac ttcacgcatt acgttaagca tccgttataa tcggttgcag
     7321 ataccagcct gtggatgctt aacatggaat accaactcac tcttaactgg cccgattttc
     7381 ttgaacgtca ctggcagaaa cgcccggtgg tgttaaaacg cggctttaat aattttattg
     7441 acccgatctc tccagacgag ttggcgggtc tggcgatgga aagcgaagtt gacagtcgac
     7501 tggtcagtca ccaggatggc aaatggcagg tcagccacgg cccgttcgaa agctacgatc
     7561 atctcggtga aaccaactgg tcattactgg tacaggcagt aaaccactgg catgagccga
     7621 ccgccgcgct gatgcgaccg ttccgtgaac taccggactg gcgtattgat gatctgatga
     7681 tttctttttc tgtacctggc ggcggcgtcg gcccgcatct cgatcagtac gacgtgttta
     7741 tcattcaggg taccggacgt cgtcgctggc gagtgggcga aaagctgcaa atgaaacagc
     7801 actgcccaca tccggatctg ttacaggtcg atccgttcga agccatcatc gatgaagagc
     7861 tggagcctgg tgatattctt tatattccgc caggattccc gcatgaaggc tacgcgctgg
     7921 aaaatgcgat gaactattcc gtgggttttc gcgcgccaaa tacgcgggaa ttaattagcg
     7981 gatttgccga ttatgtgctg caacgtgaac tgggcggcaa ctactacagc gatccggatg
     8041 ttccacctcg cgctcatcct gcggatgttc tgccgcaaga gatggataaa ctgcgtgaga
     8101 tgatgctcga attgatcaac cagccggaac actttaagca atggtttggc gagtttatat
     8161 cccagtcacg tcatgaactg gatatcgcgc cgccggaacc gccttatcag ccagatgaaa
     8221 tctacgatgc gctgaaacaa ggtgatgtgc tggtgcgcct gggtggtctg cgcgtattgc
     8281 gcattggcga cgacgtgtat gccaatggtg agaagatcga ttccccgcac cgtccggcac
     8341 tggatgcact cgccagcaac attgcgctga ctgcggagaa ttttggcgat gcgctggaag
     8401 atccgtcatt cctcgcgatg ctcgcggcgc tggtcaatag cgggtattgg ttcttcgaag
     8461 ggtaagtttg gatttaagcc ggatgcagca tcacatgccg catccctttt cgcttacttc
     8521 cgttgcgccg ttaactcggc aatacggacg atcacctgca ccgctttttc cataccttcc
     8581 agagtcacaa actcatgctt accatgatag ttgtaaccgc cagtgaacag gttcgggcaa
     8641 ggtaatccca taaacgacaa ctgcgcgccg tcggtaccgc cgcggatcgg tttcagttcc
     8701 ggttcaatat cgcagtcacg catcgcctgc tgggcgatat cgagaatatg cggatgctca
     8761 accactttct cgcgcatatt gtagtaactg tcttcaatca ccaattcaat gtagcaatca
     8821 ggatgtaacc ctttgcccac ttttttggcg atctccatca ttttacgttt acgcgcttca
     8881 aactgtttac ggtcgaaatc acggatgatg tagtgcatat cggcccgttc aacggtgcct
     8941 ttcatgctcg ccaggtgata gaaaccttca tagccttctg tcatttccgg gctttcatcc
     9001 gccggaactt ccgcatgaat acgtgccgcc agcgacagcg catttaccat cactcctttc
     9061 gccgtgcccg gatgaacatt gttaccgaca attttgatat tgaccgatgc ggcgttgaag
     9121 ttttcaaact ccagttcgcc tacgccacca ccgtcaacag tgtaagccca gcgggcatcg
     9181 aaggcatcaa catcaaaatg tttcgcccct ttgcccactt cttcatccgg ggtaaaggcg
     9241 acgcgaatat caccatgcgg aatgtttttc tgttgcaata ccgccagcgc ggtcatgatt
     9301 tctgcaatac ctgctttgtc atcggcacct aacaaggttt taccatcggt ggtaatcaac
     9361 gtctgaccca gtagctgatg cagcaccggg aacataaccg gtgataaagc ttcatcgccg
     9421 atacccagcg caatatcgcc accgcgatag ttttcaacaa tttgcggatt aacatttttg
     9481 ccgctgcaat ccggtgaggt atccacatga gaaataaagc caatcgccgg gatatcgcca
     9541 gggacgttag ccggtaacgt cgccatcaaa gtgcccttct cacttaaggt cacattgata
     9601 agccccattt cttcgagctg ctctttcagc agatgcaata acttccattg gccttccgtg
     9661 ctgggaacct gtctcacccc tgcttttgat tgggtatcca gagacacgta gttcaaaaat
     9721 cgctcaagta gtttatccat gtagtcaccc tcactttttg tgacaacatt attaagaagc
     9781 aagaaaagac aaatattgcg tcaggtcact tttacccctg caagcgggaa tatttatcag
     9841 cattacctta atgaatataa agctaagcca gtaattcaca acaaagattg gcgattcaag
     9901 cggtttgccg tagaattacc gccctcatta agagtcacca aggtggttaa ccacaaaccc
     9961 cgcatcggta agccatccgt tgcgtttaca tgggacagag taaaaaattg aataaacaac
    10021 cgagttcgct ttcaccgctg gtgcaattgg cgggaattcg caaatgcttt gatggtaaag
    10081 aggtcattcc ccagctggat ctgactatca acaatggcga gttcctcacg ctgcttggcc
    10141 cttctggctg cggtaaaaca accgttcttc gcctgattgc aggtctggaa actgttgatt
    10201 ccggacgcat catgctggat aacgaggaca tcacccacgt tccggcggaa aaccgctatg
    10261 tgaacactgt tttccaaagc tatgcacttt tcccccacat gaccgtgttc gaaaatgttg
    10321 cctttgggtt gcgcatgcaa aaaacccccg ctgctgaaat tacgccccgc gtgatggaag
    10381 ccctgcggat ggtgcagctg gaaaccttcg ctcaacgtaa accgcatcag ctctctggtg
    10441 gtcaacagca acgcgtcgcc attgctcgcg cggtggttaa caagcctcgt ctgttgttgc
    10501 tggatgagtc gctctcagcg ctggattaca aactgcgtaa gcaaatgcag aacgagctga
    10561 aagcgttaca gcgtaagctt ggcattacat tcgttttcgt gactcacgac caggaagaag
    10621 cactcaccat gtcggacagg attgtggtga tgcgcgatgg tcgcattgag caagacggca
    10681 cgccgcgtga aatctacgaa gagccgaaaa acctgtttgt tgccgggttc attggcgaaa
    10741 ttaatatgtt taacgccacg gtgatcgagc gactcgacga gcagcgcgta cgcgccaacg
    10801 ttgaaggccg cgaatgtaat atctacgtta acttcgccgt tgaaccgggg caaaaactgc
    10861 atgttctgct gcgcccggag gacttacgtg ttgaagagat taacgacgac aaccacgctg
    10921 aagggctgat tggttacgtt cgtgagcgta actacaaagg catgacgctg gagtcggttg
    10981 ttgaactgga aaatggcaag atggtgatgg tcagcgaatt cttcaatgaa gacgatcctg
    11041 actttgacca ctctctcgac caaaaaatgg ccattaattg ggtagaaagc tgggaggtcg
    11101 tactggctga tgaagaacac aagtaagttc cagaatgtag tgattgtcac tattgtcggt
    11161 tggcttgtgt tgtttgtctt tctgcccaac ctgatgatca ttggcaccag ctttttgacc
    11221 cgcgacgacg ccagtttcgt caaaatggtc tttacgctgg ataactacac gcgtctgctc
    11281 gatccgctct attttgaagt gctattgcac tcgctgaata tggcgctgat cgccaccctc
    11341 gcctgcctgg tgctgggcta tccatttgcc tggtttctgg cgaagctgcc acacaaggtg
    11401 cgtccgctgc tgctgtttct gctgattgtt ccgttctgga ccaactcatt gattcgtatc
    11461 tacgggctga aaattttcct cagcaccaaa ggctatctca acgagttttt gctctggctg
    11521 ggcgttatcg acacaccaat ccgtatcatg ttcacgccca gtgcggtgat tatcggtctg
    11581 gtttacattc tgctgccgtt tatggtgatg ccgctgtact ccagtatcga aaaactggat
    11641 aagccgttgc ttgaggcggc gcgcgatctc ggtgccagca agttacagac ttttatccgc
    11701 atcattattc cactgacgat gccggggatt attgccggat gtctgctggt gatgctgcca
    11761 gcgatgggcc tgttctatgt gtccgacctg atgggcggtg cgaaaaacct gctgatcggt
    11821 aacgtcatca aggtacagtt cctcaatatt cgtgactggc cgtttggtgc agccaccagc
    11881 attacgctga ctatcgtaat gggcctgatg ttgctggttt actggcgcgc ttctcgtttg
    11941 ctgaataaga aggtggaact cgaatgatcg gtcgactgct tcgcggcggt tttatgaccg
    12001 ctatctacgc gtacctgtat atcccaatca ttattttgat tgtgaactcc tttaacagct
    12061 cgcgctttgg catcaactgg cagggtttta ccaccaaatg gtatagcctg ctgatgaaca
    12121 acgacagcct gttacaggca gcgcagcatt cactaacaat ggcggtgttt tcggcgacgt
    12181 ttgctacgct tatcggttca ctgacggcag ttgcgctgta ccgttatcgc tttcgcggta
    12241 agccgttcgt tagcggaatg ctgtttgtgg taatgatgtc accagatatc gtgatggcaa
    12301 tttcgctgct ggtgctgttt atgctgctgg gtattcagct tggcttctgg tcgctgctgt
    12361 tctcgcatat caccttctgc ctgccatttg tggtggtgac ggtgtattcg cgcctgaaag
    12421 gttttgacgt gcggatgctg gaagcggcga aagatctcgg tgccagcgaa tttaccattc
    12481 tgcgaaaaat cattctgcca ctggcaatgc cagcggtggc ggcgggctgg gtgttaagct
    12541 ttaccctgtc gatggacgat gtggtagttt cttcgttcgt caccggacca agttatgaaa
    12601 ttctgccgtt aaaaatttat tcgatggtca aagtcggcgt atcgccggaa gttaacgcgc
    12661 tggcaaccat attactggtg ctgtcgctgg tgatggttat tgccagccag cttattgctc
    12721 gtgataaaac gaaaggtaac acaggggacg ttaaatgaaa aaatggtcac gccacctgct
    12781 cgcggcgggt gctctggcac tgggcatgag cgccgctcac gccgatgaca acaacacgct
    12841 gtatttctac aactggaccg agtacgtgcc gccaggactg cttgaacagt tcaccaaaga
    12901 aaccggtatt aaggttatct attcgactta cgagtcgaac gaaaccatgt acgccaagct
    12961 gaaaacgtac aaagacggtg cctatgacct ggtggttcct tcaacctatt acgtcgataa
    13021 aatgcgtaaa gaggggatga tccagaagat cgacaagtcg aagttaacga acttcagcaa
    13081 tctcgatcca gacatgctca acaagccttt tgacccgaat aacgactact ccattccgta
    13141 tatctggggt gcgacggcga ttggtgttaa cggtgatgcg gtggatccga aatctgtcac
    13201 cagctgggcc gatctgtgga agccagagta caaaggcagc ctgctgttga ccgacgatgc
    13261 ccgtgaagtg ttccagatgg cgctgcgtaa gctgggctac tccggtaaca ccaccgatcc
    13321 gaaagagatt gaagctgcat ataacgagct gaaaaaactg atgccaaacg tcgcagcgtt
    13381 taactccgat aacccggcta acccgtacat ggaaggcgaa gttaacctcg gcatgatctg
    13441 gaacggttct gctttcgttg cacgccaggc gggtacgcct attgacgtgg tgtggccgaa
    13501 agaaggcggc atattctgga tggacagcct ggcgatcccg gcaaatgcca aaaacaaaga
    13561 aggcgcgctg aaattgatca acttcctgct gcgcccggat gtggcaaaac aggttgctga
    13621 aactatcggt tatccaacgc caaaccttgc ggcgcgtaag ctgttaagtc cagaagtggc
    13681 aaacgataag acgctctacc cggatgctga aaccattaaa aatggcgaat ggcagaatga
    13741 cgttggcgca gccagcagca tttatgaaga gtattatcag aagctgaaag caggacgtta
    13801 attcagcaag cgggcggtgg tattaccgcc cgttttttag ttagcaagga tgtggctaat
    13861 gagtcaggac agtaaagttt tatcccgctc tttcgtcggt attgtggtcc gcttgtaggc
    13921 ctgataagcg tagcgcatca ggcaatgctt cctgctttat aagcccttca gcaacttttc
    13981 aacaaattct ggcaccacct ggcttgccgg gccgtaatat ttctcggcaa attcattacc
    14041 aacctgactc ggttcaagat tcagttcaac ggtgtgagca ccatgtagtt tggcttcgtg
    14101 tacaaaccca gccgccggat agacatgccc ggaagtacca atggcaataa agacatccgc
    14161 catcgacaac gccatataaa tttcatccat gccgagtggc atttcgccaa accaaacgac
    14221 gtgcgggcgc aagggtgccg ggaactggca acaatggcat ttatcttctg gcgtaacgtc
    14281 tccggtccag tcgagaacct gaccactttg cgaacaacga actttcagca attccccatg
    14341 catatgaatg acattggtat tacctgcgcg ttcatgcagg ttgtctatat tctgcgttac
    14401 cagcaaaaag cgatcgccga gagcgtcttg cagtttagcc agcgcaagat gcgcggcgtt
    14461 aggctgaatt tctggctgct gcagctgtcg acgacgggcg ttataaaacg cttgcaccag
    14521 ttcaggatcg cgatcgaaac cttccggcgt tgccacatct tcaacccgat gttcttccca
    14581 caggccatct gcggcgcgaa aggtacgaat acctgattcc gcagaaattc ctgcccctgt
    14641 cagtacgagt actcttggtt tttccattgc ttccggcacc actttatctc tgaaaaaaat
    14701 acgctgacgc aaacgctcgc gcaggcggcg tttattttta cgaaaacgac ttaaccgatg
    14761 accccgacgc gacaacatag caacctctgt tgtttaatcg gttagatgta ggaaggccgc
    14821 accacgcatt cctcccgcat caccgtggcg cgcgcgttca atgcgcggaa cacgagctac
    14881 aggtaagaga tgacaaggca acctgtccgc cagttgcgtt gtgattgccg ggaaattcga
    14941 taagccacca ccaatgacga ccaggtcagg gtcaacaatg gtcaggatat ttcccagaga
    15001 aaccgctaat aaatccagat aacgctcaac gtgcgccctt gcctgctcat cgccttgatc
    15061 ataaagcgca ataatttcgg gagcctgcaa cggttgatga taatagtgtt gatacagcca
    15121 cgcaaaaccg cgaccagaca gataattttc aatgcagcca tgctgaccac agccacagcg
    15181 gcgtaacggg aaatccaacc ccatcatggt taacgcatca accggcagac gcatatggcc
    15241 aaactcgccg gtaatgtagc ttttaccagt aatcggtttg ccgttgaaaa tcagcccgcc
    15301 gccaacgccg gtgccgagaa ttaaccccat caccagtgga tattgcgtaa attcatcatc
    15361 ccaggcttct gaaagggcaa aacagttggc atcgttatca aggcgtacat cgcgatcaag
    15421 acgtgcgctc aggtcggcac gcagcggttt gccgctggca gcggggacat tagcggcata
    15481 cagcttacca tcttctgttt ccggcattcc cggaataccg atgccgacag aacctttaca
    15541 gccaaaacgt tggtcagctt cagctaccag ctcacacact gcatctaaaa atgcgtcata
    15601 gctgtcacgc ggtgtcggca cccgcttttc ccactgcaac tgccgaccgc tatcaaacac
    15661 gccaagcgca atttttgttc caccaatatc aaacccgtaa tacattgccg ctccttgttt
    15721 taatgtactg cctttactgg ccgctaagga ctcgcgcagg gtcaatatta ctggcgcgcc
    15781 gcgccggata ccaacttgcc aaaagactca gcaacaatgc tgtgaccagt acgtagaaga
    15841 cgtccagcca gtgcaattcc gatggcagga agtcaataaa atagatatcg ctggagagga
    15901 actgatgacc gatcagcttt tcaatccact caataatcgg ggtaagttgc agtgaaacaa
    15961 ctacgccgat aaccacccca cacaggctgc cgaacagccc tgccagcaat ccataccaga
    16021 caaagatggc gcgaattaaa ccatctttcg cccccagcgt tcttaatact gcgatatcgc
    16081 cactcttgtc tttcaccgcc atcactaagg tggagacgat gttgaaacag gccacgccaa
    16141 tcaccagtac catcgccaga tacataatgg cgcggatcat ctggatatcg cgatacatat
    16201 agccgtaagt accaatccaa cttttaatat aaacatagct gttggtcact tcacccgcat
    16261 cgcgtaccag cttattggcg ttgaaaacat ccgtcatttt aagggcaata cctgacacgc
    16321 tggaacccat atcaagatat tgttgggcat ccgccagcgg gatcatggca aaactgtgat
    16381 cgagttgacc actcaactgc aaaataccgg caatgtgcaa acgcacacgt tttggctgca
    16441 tcagtttatg ctcgggattc gagttgggga tcatgatcga cacccaatcg ccctgcttca
    16501 ctttcagcgc atccgccacg cctttgccga tgataatttg ctgttcgccc gctttaaaat
    16561 tgcgccacgc atccccctga acaaacgagg gtaatgcgct cagacgctgt tcctgttgtg
    16621 ggttaacgcc cttcacctgg attgcgcgca gattcgctcc actttccacc agaccggtga
    16681 aattgatata cggcgcagcg gcggcaatac ccggcacttt ctgcacgtta tccagtgctt
    16741 cctgccagtt agtccatggc tgatttaccg cttcgatttc gccatgcggc accaccgcca
    16801 gaatgcggtt attcagttcg cgttcaaagc cgttcatcgc gcttaagccg acgatcaata
    16861 ccgccacgcc gagggcaatg ccaatggtag aaatgacaga gattagcgac accatgccgc
    16921 cgcgtcgccg tccgcgacta aaacgcaggc caatcaataa cgataaaggc atcgccatta
    16981 ctccgccccc atcaggctca gttccgccgt cagacgtcca tcacgcattt ccagttggcg
    17041 gctcatacgt ttcgccagtt gcaggtcgtg agtgaccacc aggaaagcgg tgccctgcaa
    17101 gcgattcaac tccccaagca actggaagat gctgtcggcg ttacgcgcat cgaggttacc
    17161 ggtaggttca tccgccagta ccagacgcgg attattgacc agcgcacggg caatcgctac
    17221 acgctggcgt tcgccgccag aaagttcaga tgggcggtga ttcgcacgat gctccagccc
    17281 caccgctttt aacatctcca gtgcacggct gttgatttca gcgggctttt tcttgccaat
    17341 cagcagtggc atagccacgt tttccagggc agtaaaatcc ggcagcaggt ggtgaaactg
    17401 ataaataaag cccagcttct ggttgcgcag ttctgctttc gccgctgaag acagtttgct
    17461 catcggctga ccgttaaaga tcacatcgcc ggaggttggc gtatccagcc cgcctaacag
    17521 gtgcagcaag gtacttttac cggaaccaga gctaccgaca attgccatca tttcaccttc
    17581 gcccacgctg aaactgacat tgtgcagcac atcggtttgc acactgcctt cctgatagcg
    17641 tttgcacagg ttgtcgcatt gcaacaggat cttattcata acgtaaggcc tcagcgggtt
    17701 gtgtggcggc agcgcgccat gaagggtaaa gcgtagacag cagcgcgata gccatcgcca
    17761 ccagcgcaat aacaatgacc tgtaaaggtt cgatagccac cggcagcgcc gcgccatcaa
    17821 gcaggacgcc gattatcggc atcagattat ttaactggct ggcaagcagt gcgccaagcg
    17881 ccgctccgag gattgcaccg ataatcccgg cgctggcccc ttgcaccata aagaccatca
    17941 tgatttgtcg cggagttaag ccttgcgttt gcaggatcgc gacttcgccc tgcttctcca
    18001 tcaccatcag ccccagcgag gtaataatgt taaacgccgc aacagcgaca atcaggctca
    18061 gcagtaagcc catcatattt ttttccatgc gtacggcctg gaacagctcg cctttacggt
    18121 cacgccagtc ctgccatttg ctgccttcag gcagtttttg ctgacttaaa gagtcaactt
    18181 tcagcggctc atccagccac aaacgccagc cggtaatatt ggctgccgga taacgcatca
    18241 gacgcgaggc atcctcaata ttcaccagca tttcatagcc atcgacttca ctgttggcgg
    18301 cgaaagtgcc aatcacattg aacaggcgct gacttggaat acgccccatc ggcgtgaact
    18361 ggctggcaga tggcaccatc acgcggattt gatcaccgcg attgacgccc aactgtgagg
    18421 caagttgttc gccgaggatg acattatatt tccccggctc gaggtcagtt tgtttcacat
    18481 tgaccagata cggcgttagt ggatcttttt gcgccgggtc gataccgagc atcaccccga
    18541 ccgccacgct gcgcgcgctt tgcagtacca catcaccggt agtaataggt gcgacgcgat
    18601 taacgccgtc cagtttgact gccgtttccg ggagttgctg cgggttaaga gagccatgct
    18661 cagaagagag aattgcctgt ggcatcaggc caaggatgtt gttttgcagc tcgcgctcaa
    18721 agccgttcat cactgacaat actgtgacca gcgccatcac cccgagggta atgccgatgg
    18781 tagaaagcca ggagacgaaa cgaccgaagc gatccgctgc acgcccacgc atgtaacgca
    18841 ggccaataaa tagagcgaca ggttggtaca tgaaatccgt ctggttgctg tagcaaagac
    18901 acgagtatat aagcgaaagc aagatgggta aacgtctgaa ctgtaagtga tcgtcagggc
    18961 taatctgaaa agcccccgta aatcaatcgg ctaaattgcc atgttttatc atcaatcttg
    19021 ccgcctgatc ttaatctgaa aaatcatcca atacagacag atacccatta cacctcgggt
    19081 cataaccagc aggatcaacc gcatgtaaat taaaaaagag cgtgttgtcc tgatgaaaca
    19141 aaaagagcta tggattaacc agatcaaagg gttatgtatt tgtctggtgg tgatttatca
    19201 ctcggtcatt accttttatc cgcatatgac cacttttcag catccgttat cggaagtcct
    19261 gagcaaatgc tggatctatt tcaatcttta ccttgccccc tttcgtatgc cggttttttt
    19321 ctttatatcc ggctatttga ttcgccgcta tatcgacagc gtaccgtggg gaaattgtct
    19381 cgataaacgg atctggaaca tcttctgggt gctggcgctt tggggcgtgg tgcagtggct
    19441 ggcgttaagt gcgctgaacc agtggctggc acctgagcgc gatttaagca atgcctccaa
    19501 tgccgcttat gccgattcca ccggtgagtt cctgcacggg atgatcaccg ccagcaccag
    19561 cttatggtat ctgtatgcgt taattgtcta tttcgtggta tgtaaaattt ttagccgtct
    19621 ggcgctgcca ctattcgcct tgtttgtact gctgagtgtg gcggttaatt tcgttcccac
    19681 gccgtggtgg ggaatgaaca gtgtgatacg caatttgcct tattacagcc ttggcgcatg
    19741 gtttggcgca acaataatga cctgtgttaa agaggtgccg ttgcgccgcc atctgctgat
    19801 ggcttctttg cttaccgttc tggcggtcgg tgcctggttg tttactatct cgctgctgtt
    19861 gtcgctggta tcgattgtgg tgatcatgaa gctgttttat cagtacgagc aacgattcgg
    19921 tatgcgctcc accagcctgc tgaatgtgat tggctccaac accattgcta tctacaccac
    19981 ccatcgcatt ctggttgaaa tattcagctt aactctgctt gcgcaaatga acgcagcacg
    20041 ctggtcgccg caagtcgaac tgacactcct gctggtttat ccctttgtca gtttgttcat
    20101 ctgtactgtt gcgggcttgc tggtaagaaa actttcacag cgtgcattca gcgatctgtt
    20161 gttctccccg ccttcactgc ccgctgccgt cagttactct cgttaaacca tcgccccagc
    20221 cggggcgatt caatttgcta aaccatgtcg attacggata atgaacagca ttgcttatca
    20281 gagatatgcc cccatatgtt gaggcatatc ctaacgagaa tctgacaacc gttatgcctg
    20341 aacaatatcg ttatacgctg cccgtcaaag cgggtgagca gcgtctgctg ggcgagttaa
    20401 ccggcgcagc ctgtgcaacg ctggtagcgg aaattgccga acgtcacgcc ggtccggtgg
    20461 tactcattgc accagatatg caaaatgctc tgcgtttgca tgatgaaatc agccagttca
    20521 ccgatcagat ggtgatgaat ctggcggact gggaaactct tccctacgac agtttttcgc
    20581 ctcatcagga cattatctcc tcgcgccttt ccacccttta ccagctaccg acgatgcagc
    20641 gtggcgtact gattgttccg gtgaatacgc ttatgcagcg cgtttgccca cacagttttc
    20701 tccacggtca tgcgctggtg atgaaaaaag gtcagcgcct gtcacgagat gcattacgaa
    20761 cccaactgga cagcgccggt tatcgccatg ttgaccaggt gatggtgcac ggcgaatacg
    20821 ccacgcgcgg cgcgttgctg gatctcttcc cgatggggag tgagctgcct tatcgtcttg
    20881 atttctttga tgatgaaatc gacagcctgc gggtgtttga cgtcgacagc cagcgcacgc
    20941 tggaggaagt agaagcgatc aatctgctgc ccgcgcacga atttccgacc gataaagcgg
    21001 caattgaact gttccgcagc cagtggcgcg ataccttcga agtgaagcgc gatccggaac
    21061 atatttacca gcaagtgagt aaaggcacat tacctgccgg gatcgagtac tggcagccat
    21121 tgttcttcag cgaaccactg ccgccgctgt tcagttattt ccctgccaat accttgctcg
    21181 tgaatactgg cgatctggaa accagtgccg aacgtttcca ggctgacacg ctggcgcgtt
    21241 ttgagaatcg cggcgtcgat ccgatgcgcc cgctgttgcc accacaatcg ctctggctgc
    21301 gggtggacga gctcttctca gagctgaaaa actggccccg agtgcagcta aaaactgaac
    21361 atttaccgac aaaagccgcg aatgccaatt taggtttcca gaaactgcca gacctggccg
    21421 ttcaggcgca acaaaaagcg ccgctggatg cgctgcgtaa gttcctcgag actttcgacg
    21481 gtccggtggt gttctcggta gaaagtgaag gtcgccgtga agcgctgggt gaactgctcg
    21541 cacgaattaa aattgctccg caacgcatta tgcgtcttga tgaagccagc gaccgtgggc
    21601 gttatctgat gattggcgct gccgaacatg gttttgtcga taaggtgcgt aatctggcgc
    21661 tgatctgcga aagcgatctg ctcggtgaac gtgttgcccg ccgtcgtcag gattctcgcc
    21721 gcaccatcaa ccccgataca ctgatccgta accttgcgga gctgcatatt ggtcagccgg
    21781 tggtccatct ggagcacggc gtcggtcgct atgccggaat gaccacgctg gaagctggcg
    21841 gcattactgg cgagtacctg atgctcacct atgccaacga cgccaaactg tatgttccgg
    21901 tgtcgtcact gcatctgatt agccgttacg caggtggcgc ggaagaaaac gccccgctgc
    21961 ataaacttgg cggcgatgcg tggtcacgcg cgcggcagaa agcggcggaa aaagtgcgtg
    22021 atgtggcggc ggaattgctg gatatctacg cgcaacgcgc cgccaaagag ggcttcgcgt
    22081 ttaaacacga tcgtgagcag tatcagttgt tctgcgacag cttcccgttt gaaaccacgc
    22141 cggatcaggc gcaggccatt aatgcggtac ttagcgacat gtgtcagccg ctggcaatgg
    22201 atcgtctggt gtgcggcgat gttggctttg gtaaaacaga agtggcgatg cgcgccgctt
    22261 tcctggcagt agataaccac aagcaggtag cggtgttggt gcctaccacc cttcttgcgc
    22321 agcagcatta cgacaacttc cgcgaccgtt tcgccaactg gccggtacgt atcgaaatga
    22381 tctcccgttt ccgcagcgcc aaagagcaga cgcaaatcct tgcggaagtg gcggaaggga
    22441 aaatcgatat tctgatcggt acgcacaaac tgctgcaaag tgacgtcaag tttaaagatt
    22501 taggcctgct gattgtcgat gaagaacacc gcttcggggt gcgtcataaa gagcgcatta
    22561 aagcgatgcg cgcgaacgtg gatattctga cgcttactgc aacgccgatc ccacgtacgc
    22621 tgaatatggc aatgagcgga atgcgtgacc tgtcgattat cgccacgccg cccgcccgtc
    22681 gtctggcagt taaaaccttt gtccgtgagt atgacagcct ggtggtccgg gaggcgatcc
    22741 tgcgtgaaat tttgcgcgga ggacaggttt attatctcta caatgatgtg gaaaacattc
    22801 agaaagccgc cgaacggctg gcagaactgg tgcctgaagc acggattgcc atcggtcacg
    22861 ggcagatgcg cgagcgcgaa ctggaacggg tgatgaatga tttccatcat caacgtttca
    22921 acgtgctggt ttgtaccacc attatcgaaa ccgggatcga catcccgaca gccaacacca
    22981 ttatcattga acgtgcggat cacttcggtc tggcgcagct gcaccagtta cgcggtcgcg
    23041 tcggacgttc acatcatcag gcatatgcat ggctgctgac gccgcatcca aaagcgatga
    23101 ctaccgatgc acaaaaacgt cttgaagcga ttgcctcgct ggaagatctc ggggcaggtt
    23161 ttgcgctggc aacgcacgat ctggagattc gcggcgcggg tgaactgctt ggcgaagaac
    23221 aaagcggctc aatggaaacc atcggtttct cgctgtatat ggagttgctg gaaaacgccg
    23281 tcgatgcact gaaagccgga cgcgagccgt cgctggaaga tctcaccagc cagcaaacag
    23341 aagtcgagct gcggatgccg tcgctattgc cagatgattt catccctgac gtgaacacgc
    23401 gtctgtcgtt ctacaaacgt attgccagcg ccaaaacgga aaacgaactg gaagagatca
    23461 aagtcgagct tatcgatcgc ttcggcctgc tgccagatcc ggcgcgtacc ctgctggata
    23521 ttgcccgtct gcgccagcaa gcgcagaaac tggggatcag gaagctggaa ggtaatgaga
    23581 aaggcggggt gatcgaattt gccgagaaga atcacgttaa tccggcctgg ttgattggtt
    23641 tgctgcaaaa acagccgcag cattaccgcc ttgatggtcc gacgcgcctg aaatttattc
    23701 aggatttgag tgagcggaaa acgcgtatcg aatgggtacg ccagtttatg cgtgaactgg
    23761 aagagaacgc gatcgcttaa tgtaaatcgg gggcgtttct gcgcccccat ctgcaacatt
    23821 tacaaattct ttgcacttcc ctgcactatc cgacactgtc accatccata attcaggctt
    23881 atctgtttat tacaataacc ttatatttat tatggatttt tggtgatgat caaaacgcgt
    23941 ttttctcgct ggctaacgtt ttttacgttc gccgctgccg tggcgctggc gctaccggca
    24001 aaagccaaca cctggccgct gccgccagcg agcagtcgtc tggttggcga aaacaaattt
    24061 catgtggtgg aaaatgacgg tggttctctg gaagccatcg ccaaaaaata taacgtcggc
    24121 tttctcgctc tgttacaggc taaccccggc gttgatcctt acgtaccgcg cgcgggcagc
    24181 gtgttaacga tcccgttgca aaccctactt ccagacgcgc cgcgcgaagg cattgtgatc
    24241 aacattgcgg agctgcgtct ctattactac ccgccgggta aaaattcggt aaccgtgtat
    24301 ccaataggta ttggtcagtt aggtggtgac acgctgacac cgacaatggt gaccacagtt
    24361 tcagacaaac gtgcaaaccc aacctggacg ccaacggcaa acatccgcgc ccgttataaa
    24421 gcacagggaa ttgagttgcc tgcggtagtg ccggctggac cggataaccc aataggccat
    24481 catgcgattc gtctggcggc ctatgacggc gtttatttgc ttcacggtac gaacgccgat
    24541 ttcggcattg gcatgcgggt aagttctggc tgtattcgtc tgcgggatga cgatatcaaa
    24601 acactcttta gccaggtcac cccaggcacc aaagtgaata tcatcaacac tccgataaaa
    24661 gtctctgccg aaccaaacgg tgcgcgtctg gttgaagtac atcagccgct gtcagagaag
    24721 attgatgacg atccgcagct gctgccaatt acgctgaata gcgcaatgca atcatttaaa
    24781 gatgcagcac aaactgacgc tgaagtgatg caacatgtga tggatgtccg ttccgggatg
    24841 ccggtggatg tccgccgtca tcaagtgagc ccacaaactc tgtaaatcct ggataaaaaa
    24901 aatccccgcg acacggcatg tcgcggggaa aaaatcagca gtagcaggca ttaatgaggg
    24961 ttaatgctta tttataaatt actgctgttc catggagggt attcggaccg gttacagaag
    25021 taatacggaa agattttgcg cccatctcat ccgctttttg cgccagctgc tcttccagcg
    25081 atcccagatt tgtccccgcg ttagcactga tagtaccgac tttttgttgg ccttctggcg
    25141 ttgactgaac ttcgacagcc gcaaagttgg caaatgacat ggagcttaaa atcgccgcag
    25201 cgatgagggt ttttacgttt ttcataatag tggccttatg cagatgaatg acgtcgcaag
    25261 aaattactta acgatcgata actaaatcga tagatgtgat ctggatcaca tacaagatat
    25321 tttttataac aatcattaat taatttaaaa atctttaaat ttcagttaaa tagaaagcaa
    25381 ttatttttat gtgatctgga ctggtgaacg gaggtgatta tttttataat aacctttcat
    25441 tcacagtcac caggtacaac ggcatcatgg caactgactc aacacaatgt gtaaaaaaaa
    25501 gccgtggccg cccaaaagtg ttcgacaggg atgccgcgct tgataaggcc atgaaattgt
    25561 tctggcaaca cggttatgaa gcgacttctc ttgcggacct cgtcgaagcg accggagcca
    25621 aagcgcccac gctatacgcg gaatttacca acaaagaggg gttatttcgt gccgttctcg
    25681 accgctatat cgatcgtttt gccgctaagc atgaagcaca gctgttttgt gaagagaaaa
    25741 gcgtggagtc tgcgctggct gactattttg ctgccatcgc caactgcttt accagcaaag
    25801 acaccccggc aggctgcttc atgatcaaca actgcaccac cctctcccca gattcaggag
    25861 atatcgccaa tacgttgaaa tcacgccatg cgatgcaaga gcgcactttg cagcagtttt
    25921 tatgtcaacg acaagcgcgc ggggaaatcc cgacccactg tgacgtgaca catctggcag
    25981 aattccttaa ttgtattatt caggggatgt cgatcagcgc acgcgaaggt gcatcgctgg
    26041 aaaaactgat gcagattgcc ggaacgactt tgcgtttatg gcccgaactg gtgaaataag
    26101 tcgcaaaaaa agcccctcaa cctgagcgaa tgaggggcca aattgcagag tacagccttg
    26161 ttatactttg ttattcaaaa tcagttgccc gttactatct agcgggatct gcgtacccgg
    26221 atcgcggtcc atgcggattt tgccctgctg atcgccaatc ttataggtca catcataacc
    26281 gagcattttt tctgacttgt catacaccgt tttacaacgc tgttgcgtag tcgtgtaagt
    26341 atcgctttcc tggagagagc cctggatctg gttaccggca tatccaccac ccagcgcccc
    26401 cacaacagtg gcgacatctt taccgcgacc accaccaaac tgatgcccta tcacgccacc
    26461 agcaacagcg ccgagcaccg acccggtaat gcgattttca tcctgcaccg gtcgacgatg
    26521 ggtcactgtg acgttgcgac actcctgacg cggtgtttta accgtttcct tgattggggt
    26581 tgcagaaaca acctgagcgt attgcgggcc ccgttcaaac acgttcagac tggccactgc
    26641 cgctacgccc aacgcagctg cgacaccaat cccgataccc gccaacattg atttattcac
    26701 gggaacacct ccttctttgc agttatgcca atcatgcaag cgaaggagaa aagcagctat
    26761 cagacagaag atgaaaagcg cgggaatgac tggaaatact taaggattcg gaggagtctg
    26821 aaggaatgcc tgatgcgacg cttaacgcgt cttatcaggc ctaccgctcg taccatttgt
    26881 ccagggcgga taaggcgttt atgccacatc cgccagtgta cgtcgattaa tgcaacttca
    26941 aacgcggacg gataacgcgg ttaatactcc ccaccagcat cattaatccg gttttaaagt
    27001 aaccatgcag cgcaatctga tgcattcggt atagcgagat atatacaaag cgcgcaattc
    27061 gtccttcaat catcattgag ccgcgcgtca ggttacccat caggctacca acggtggaga
    27121 agttcgacag cgataccagc gaaccatgat ctttatactg ataatttttc agcggcttac
    27181 cgttcatctg cgccagaatg ttgttcattg cgcaagtcgc catctggtgt gcagcctgag
    27241 cacgcggcgg aacaaagccc ccttccggac gcgggcatga cgcgcagtcg ccaatagcgt
    27301 aaatgtctgg atcgcgagtg gtttgcagcg tcggttccac caccagctgg ttgatacggt
    27361 tagtttcaag accaccgata tctttcagga agtctggcgc tttgatcccg gctgcccata
    27421 ccatcagatc agcctcaata tattcgccat ctttagtgtg caggccgcct tcatcagcac
    27481 tggtgaccat ggtttgcgtc agcacgcgaa cgccaagttt cgttagctcg ttgtgggccg
    27541 cagcagagat acgtggcggt aatgcaggca aaatacgttc tcccgcttct accagcgtta
    27601 cgttcagggc ttcgttggtc aggcctttgt aaccgtagct gtgcagttgc ttgaccgcgt
    27661 tgtgcaattc agcggagagt tctacacccg tcgcgccgcc gccgacaatc gcaatgttca
    27721 ctttgccatt tgcgcccagg ttggcagagt atttcaggaa cagattcagc atctcctggt
    27781 ggaagcgacg cgcctggtgc gggttatcga ggaaaatgca gttctctttg acgcctggcg
    27841 tattgaaatc gttagaggtg ctacccagcg ccatgaccag ggtgtcatag gcgattttac
    27901 gttccggaac cagcagttca cctttctcgt cgcgtagttc tgcaatagtg attgttttcg
    27961 cttcacgatc aatatcaatg acggaaccca actggaactg gaaaccatga ttgcgcgcat
    28021 gggccagata gctcaacgca tcgacgcctt catcaagcga gccagtcgcc acttcgtgca
    28081 gcagcggttt ccacaggtgg ctgtggttac gatcgaccag cgtaattttg gcttttttct
    28141 tacgtcccag cttatgcccc agctgtgttg ccatttccag cccaccagca ccgccgccga
    28201 caatcacaat ctttttcaat ggcgtagtca acgtgacccc cttaaattta ttaaccaatt
    28261 gttaattaat ggttattaac atagctctta attaacaaca ggttacagca atgaaaatgt
    28321 tcttcgctat tgagaataac atgaatggtg cattggtcat accaaaattg atgttaatca
    28381 agttttgttg cttaaaagat aggcaacttt accgggtgag atcaaaaaat agagtttatt
    28441 gtttattatt tgagcaaatt cggaagagta atatagggtg aagcgtatat cgagggcgtt
    28501 tggtcgtcct gataagatgc gcagcatcgc atcaggcatt gatgctcgac tgtcggatgc
    28561 ggcgtggacg ccttatccga cttacagaat tcggagcgca tcaggtaccg gcaaggttac
    28621 ctgatgcgac gagggcattt acccgagggt tttgaacgct ttaatgcgct gtaaatgcgg
    28681 ggagatattc ttgaatttgt gcgtctgctc ttcgtcccag acaatctcgt aataatgatg
    28741 caactcttca gatgtccgct ggctgttaag tgcttcatca ttacgcgaca aaataaccag
    28801 acaacgatcg cgattcttct cgcggaagtt ggtcacacac ttagtggcaa tatcggcata
    28861 ctcttccggg cgatcaatct tgccttccat gttctcataa gggaacaaat tagggttgaa
    28921 gatcacctgg cggatatcgc aaagaaaacc aatccgttcc gcccagtatc cgcccaggcc
    28981 aacgccgcaa attagcggac gctcgtcaac gttcagttgc aacattttgt ccacttcttt
    29041 aagcagatgc tgcatatcat gtttcggatg ccgcgtactg tagcttatca agcgtacatc
    29101 cgggtcaata aactgcaatt gtaagacttt ctcgtggtta cccggactgt tagagtcaaa
    29161 accgtgtaaa tagataatca tcgcttcctc acataagcca gggttagtga cctgctttct
    29221 cttcctgcca gcgttcatgt aactgattca gacgggtgct gatcgctttc cagcgagccg
    29281 agtccatcag ttcctgtcgc gaaaatgaac ctttatgata caaacgtgta acacgttctg
    29341 ccttgatcgg tgacagatta tctaacacgc tgacggcccc tttacgatta ttgcagacca
    29401 ggatcatatc gcaacccgca tccagtgatg cctgcccgcg ttcggcataa ctgcccataa
    29461 tcgcggcacc ttccatcgat aaatcgtcag agaaaatcac gccgtcaaaa cccagttcct
    29521 gacgcaaaac ggttttcagc cagtagggag aaccgctcgc cggacgcgga tcaacatcac
    29581 tgtagatcac atgcgcaggc ataatggcgt cgagtttatt ttcgcggatt aaggaactga
    29641 agaccgacat atctttagcg cgaatctccg cttgtggacg tggatcgcac ggtgtttctt
    29701 tgtgtgagtc tgccgttact gcaccgtgtc ctgggaagtg tttcccggtc gttttcattc
    29761 cggcttcatg cataccatca ataaaccggc tggcaattgc cagggctttt tgtggatcgg
    29821 catgataaga acgctcgcca atcgccgcgc tgatatgccc gacatccagc actggcgcaa
    29881 agctgatatc aatatccata gcgatcattt cgctggccat cagccaaccc gcctcttgcg
    29941 ccagtttgcc gccctcttcc attcccaaca gcgcagcaaa ggattgtgct gccggtaagc
    30001 gggtaaaacc ttcgcgaaaa cgctgcacgc gtccaccttc ctgatctacc gccaccacca
    30061 gatgattgcg cgatgctgcg cggatctggc gcaccagttc acgtaactgg gcaggatcat
    30121 gatagttacg cgtaaagaga atcagccctc ccaccagcgg atgcgccagt atttcacgct
    30181 cttccgcgtc cagttcgtaa ccttcgacat ccaacattac tggacccaca ctgctctcct
    30241 tattgtttta ttaatagctg ccgccaggtg tcatcggcca gcctgataaa ttgttgatcg
    30301 ccggtttgtc gccagcggta ctcaaaccac cctcctttga gcatcagcag ccagggaaac
    30361 catcgcctga cctgacgcca taattgcgcc ggataaatct tcgcgcgagt ggcatagtca
    30421 ttgaccaatt gccggtgctg ttcagtattt tccacccaca ccgccgccag ttccagcgcg
    30481 atatcaccat ctccggcata ctcccagtcg atgagtttta accctgatgc gctatgcact
    30541 aaatttccgg cgtggacatc catatgcaat ggacttaagc gtaaaggtcg tggttcccgc
    30601 gctttgcgca gacgttttaa cattcgcagc caacccacag tccgccgcgc cggatcgctt
    30661 tgctgccagt acagttccag taagggcaac agcgttattc gccagccaaa acgtggttgt
    30721 tgatgtagat aatacagcaa gcctgccagt tcgttggtat ccggcaaata cgtttttacc
    30781 gcgccgggca gatagtcgac taccatccag tcacggagat ataaatgcgg cttcggtgca
    30841 atgcttgcgg gtagttgtga taaagcccga tactggcgca agaacgcgga ctgcggcgca
    30901 tcaggatcgt gcggctgacg cacaacaaaa cgctggccct gatgttcaat gagaaaacta
    30961 ccgccactaa gcccactatt aaacgtcgtg acgggatgaa actgcgggaa aaagcgcgac
    31021 agcaattcgt cgcgcgtgat gggattattg ctgcgaaacg gcacctttac ctgaccagat
    31081 aatttcgccc gtctgcacca gcatcagctg catttgtagg gtcggagcgt taacgttgcc
    31141 agaggcgctg gagtacagca cgtaatgagc gccgacattg cgggcaatgc ctatggcttt
    31201 actacgggtg cctaaactgt cctgcggcga caaacctaac tgttgcttag ccatcgacag
    31261 ttgctgggcg gaaaccaggg taaatttccc gttattagcc agcgcatttc gcagcgtttc
    31321 ggtcgcttct gcggcattca gcgaaccgtt agtacggttg ttaacgctat caaccagcag
    31381 gacgctaccc gcagtcaccc cgtcagcccc aagcatctta ctgaccatcg gctgcattgc
    31441 gccattccag tcataatggc gaatatgcgg cgcaggcggt gccgttcgat cttcgtgctc
    31501 aattgggcct agctgctgcg ggatcgtcgg caccgagggc actgtgggga caggctgttg
    31561 tggctcggct ggttgttccg gcgctggttt cacttcttct accggtgcag gttcacgttg
    31621 ccccacacac ccggcgagaa acatcgccag cgcggtaatc aaggcgtagc gactcatttt
    31681 tgtcatcaag attcacccct ttacaaatat agataaagtc tgactttgtg cgcccccaga
    31741 aaattggcgc tgccgtacag cgttaccgcc gaatgtgcgg gaatggtaac gctgcgtggc
    31801 ctttccagag gatgcatctc cagccctctg gcgtcatacc agtaaaaacg ataatgaacg
    31861 gtaacgggtt cttgcctttc gttataaagc gttgaggatg ctgaaggttg aatatcagac
    31921 gtcgaaagga cgggcttttc tgcactgatg cccgcagcca ataaagatga ctccatcacc
    31981 agcgattgct catcattcac cggaatttcc ggatgtgaac gacagcccac cagcagtaac
    32041 aacaccagag acaccagccc aaagcatcct tttctcatcg ttacagacct ttatgcgcca
    32101 gcattggtcc cagcggacgg ccacccaaca agtgcatatg gatgtggtaa acctcttgtc
    32161 cgccatggcg gttggtattc atgatcagac gatagccatc ttcggcaata ccttcttgct
    32221 cagcaatttt tgccgctacg gtgatcatgc gtcccagcgc ctgctcatgc tcagctgaca
    32281 cgtcgttcac agtcggaatg aggatattcg gaatgatcag aatatgcgtt ggcgcttgcg
    32341 gcgaaatatc gcgaaacgcc gttaccagat catcctggta gacgatatcg gaggggatct
    32401 cacgacgaat aattttgctg aatatagttt cttctgccac gacattttcc tttttcaaat
    32461 agccatgcta cgccgcgcca cactgcgagt atagagtatg gtcgagttaa acctgctctt
    32521 tcaaccttaa ctcacgattt cttaagcaaa aaatcgtttt gctgccgtat atatcgccat
    32581 tattcccatt tctgcctgcg aatgcgctga aaagacattc tctgtttggt ttacacgaca
    32641 aatatgaatg cgtatatttc tcatttgcat ttgcaaacaa aattattcgc acactaaata
    32701 taaccgctca tcaccctcct ccggcatgag cctgacgaca acataaacca agagatttca
    32761 aatgctttca acacaattta acagggataa tcaatatcaa gccatcacca aaccgtcact
    32821 acttgccggt tgcatagcac tggcactatt accttctgcc gcttttgctg caccagccac
    32881 tgaagaaacg gtgattgttg agggttcagc cacagctcca gatgatggcg aaaatgatta
    32941 cagcgtaacg tctacctctg cgggtaccaa aatgcagatg actcaacgtg atattcctca
    33001 gtcggtcact attgttagcc agcagcggat ggaagatcag cagttacaaa cgctgggcga
    33061 agtgatggaa aacacgctgg ggatcagcaa aagtcaggcg gattccgatc gtgctcttta
    33121 ttattcccgc ggattccaga tcgataacta tatggttgat ggtatcccca cctattttga
    33181 atcgcgctgg aatctgggcg acgcactttc tgatatggca ctgtttgaac gcgtagaagt
    33241 agtgcgtggc gcgacaggac tcatgaccgg gacgggtaat ccatctgcgg caattaatat
    33301 ggttcgaaaa cacgcgacca gtcgtgaatt taaaggcgat gtctcggcgg aatacggtag
    33361 ctggaacaaa gaacggtatg tggcggattt acaaagccca ctcaccgaag acggtaaaat
    33421 ccgcgcgcga attgtcggcg gctaccagaa taacgactca tggctggacc gctacaatag
    33481 tgaaaagacc ttcttctcgg gcattgtcga tgctgattta ggcgatctta cgacgctttc
    33541 agccggttac gaatatcagc gcattgatgt taatagccca acctggggcg gtttaccgcg
    33601 ctggaatact gatggcagca gcaacagtta cgatcgcgca cgcagtaccg cacctgactg
    33661 ggcgtacaac gataaagaga tcaacaaggt ctttatgacc ctgaagcagc ggtttgctga
    33721 tacctggcaa gcgacactga atgccaccca ctctgaagtc gaatttgaca gcaaaatgat
    33781 gtatgtcgat gcctatgtaa acaaagcgga tggtatgctg gttgggccat acagtaatta
    33841 tggacctggc tttgatcatg tcggcggcac cggttggaac agtggcaaac gtaaagttga
    33901 tgcgctggat ttgttcgctg acggtagtta tgaattgttt ggtcgtcagc acaatctaat
    33961 gtttggtggc agttacagca aacaaaacaa tcgttacttc agttcatggg ccaacatctt
    34021 cccggatgaa attggcagtt tctacaactt taatggcaat ttcccacaaa ccgactggtc
    34081 accacagagc ctggcgcagg acgataccac acatatgaaa tcgttatatg ctgccactcg
    34141 tgtcaccctt gccgatccgc tgcatctgat cctcggcgca cgttatacca actggcgggt
    34201 tgatacgctg acttacagca tggagaaaaa ccacaccacg ccttacgctg gtctggtgtt
    34261 tgacatcaat gacaactggt cgacctacgc cagctatacc tctattttcc agccgcaaaa
    34321 tgatcgtgac agttcaggca aatatctggc tccaatcacc ggtaacaact acgagctggg
    34381 tctgaaatcg gactggatga atagccgtct gaccaccacg ttagccatct tccgtattga
    34441 gcaggataat gtcgctcagt ccaccggtac acctatcccc ggcagcaacg gcgaaaccgc
    34501 ctataaagcg gtggatggga cagtcagtaa aggggtggaa tttgaactca acggcgcaat
    34561 taccgacaac tggcagctga catttggcgc aacgcgctat attgcagagg ataacgaagg
    34621 aaacgccgtt aatcctaatc tgccacgcac cacggttaaa atgttcacca gctatcggtt
    34681 gcctgtcatg ccagagttga cagtcggcgg tggtgttaac tggcaaaatc gcgtgtatac
    34741 cgacaccgtg acaccgtatg gcaccttccg cgccgagcaa ggtagctacg cgctggtgga
    34801 tctcttcacc cgctaccagg tgacgaaaaa cttctcgtta caggggaacg tcaataacct
    34861 gttcgacaaa acctacgata ccaacatgga aggttctatc gtctacggcg caccgcgtaa
    34921 tttcagcatt accggcacgt atcaattctg aataacacct gtaaaaaagg cagccatctg
    34981 gctgccttag tctccccaac gtcttacgga ttagtggtta cggatgtact catccatctc
    35041 ggttttcagg ttatcggatt tagtaccgaa aatcgcctga acaccagaac cagcaaccac
    35101 tacgcccgct gcgcccagtt tcttcaggcc agcctgatcc actttagaca catcagcaac
    35161 gctgacgcgc agacgggtaa tacatgcgtc gaggttagta atgttttctt taccaccaaa
    35221 tgcagcaacc agagccggtg ccatttcgct ggtacctgtt gcttttgcat cttcagtcgc
    35281 gtcttcacga cccggcgttt tcagatccag tgctttaatc agcacgcgga agatggtgta
    35341 gtaaacaatc gcataaccga taccgacgat cgggaacagc cacagtttgc tgctgttacc
    35401 agacagaacg atgaagtcga tcagaccgtg cgagaacgac gtaccgtcac gcatccccag
    35461 aagaatacag attgggaatg ccaggcctgc cagaatcgcg tggatgatgt acaggatcgg
    35521 cgcaacgaac atgaagaaga actcgatcgg ctcggtgata ccggtcagga acgaggtcag
    35581 cgccgcggag atcataatac cgcccacttt cgcgcggttt tctggtttag cagagtgcca
    35641 gatagcaatt gcggcagctg gcagaccgta cattttgaac aggaaaccac cagacagttt
    35701 acccgcagtc gggtcacccg ccatataacg cggaatgtcg ccgtggaaaa cctgacctgc
    35761 tgcgttggtg tattcaccaa tctgcatctg gaaaggtacg ttccagatgt ggtgcagacc
    35821 aaacggtacc aggcaacgtt cgatgaaacc gtaaatgcca aacgcaacta ccgggttctg
    35881 gtaagcagcc cactgagaga aggtctggat tgcagaacca atcggcggcc aaatgaagga
    35941 cagcacaacg ccagtaaaga tggcagccag gccagaaatg atcggcacaa agcgtttacc
    36001 ggcaaagaag ccaagatact caggcagctt aatacggtag aaacggttaa acatgtacgc
    36061 tgcgatcgca ccggagataa tccctccgag tacgccagta tccgccaagt gtttagaggc
    36121 gatttcttca gcaggtaaat gcagtaccag tggcgcaacc acggccatgg ttttaaccat
    36181 gatgccatag gcaacaactg cggccagcgc ggatacgcca tcgttattgg taaagccgag
    36241 ggcgacaccg atcgcaaaaa tcagtggcat gtttgcaaag acggaaccgc ctgcttctgc
    36301 cataacatgc gatacaacgg cgggcagcca gctgaaattc gcggaaccga cgcccagcag
    36361 aatacctgcg ataggtagta cggataccgg cagcatcagc gatttaccga ccttttgcag
    36421 gttagcaaat gcattcttaa acataattga gagtgctcct gagtatgggt gcttttttta
    36481 cgttctcacg cgtggcaagg ggggagagcc tcgccgtgta cagggcatct aagcgccctt
    36541 tatttattac acagagtaaa ataattcagt gccaatttgt ttgacggcta tcacgtttca
    36601 acttcagatc attggagaag ttgcacattt tgacaaaaca tatgtcacaa tatttcaaac
    36661 gcggttattc accgcccaat tttcggcggt gaactttact cgttttagcc attaattacg
    36721 agctttaaaa aaactcattc aacggatgga ttgaaggcgg gaagcgtcga tgtgaaacag
    36781 acgggcgaag ttatcggtgg ttacctgcgc cagttcttca acggcaacac ctttcaacac
    36841 agccatgtat tctgcaacgt cacgaaccat cgcaggttga ttctcttttc ctcgatgcgg
    36901 taccggcgca aggtaaggtg agtcagtttc caccagtaat cgatccaggg ggacataacg
    36961 cgcagcatcg cgcagttgct ccgcattacg gaaggtcaca atgccggaaa aggagatgta
    37021 aaatccgaga tccagtaatt tacccgccgt ttctctgtcc tctgtaaaac agtgtagtac
    37081 gccaccgcaa tccgtcactt tttcttcgcg cagaattgcc agcgtatcgg cacgggcgtc
    37141 acgggtatgg acgataaccg gcttgttcag ttcacgacca atctggatat gatggatgaa
    37201 ggactcttgc tgacgtactt tagtttccgg tgtgtaataa taatccagcc cggtttcacc
    37261 cagcgctaca acaccctctt ctgccgccag acggcgtaaa tcttctacat cgtagggatc
    37321 attctggttg agtggatgca cgccacaaga aaatacgacg ttgtcacgtt cgcctaccag
    37381 atcccgcata tgtaaataac ccggtaatgt tgtggcgact gccagacaaa atttcacatc
    37441 gcgtgcggcg gctttcgcca gaacgtcatc cacgtcctta tgcaaagatt cataatccag
    37501 accatcgaga tggcagtgtg agtcgactaa aaacatgatg tctctcttaa agatgaggaa
    37561 ccggtagcac aacgcccggt tgcaggtaat gctcaatacg cagtaaaaga tcggtgatga
    37621 gaagctcgcg gttgatgcct gtgacagaca ttaactgttc acgaatgtgg caaacatccc
    37681 ccagtatagc ctgcaggcgc gagggagaaa gatggtttgc cagttcggcg accaggcccg
    37741 gcacatcaac attggtcacc tgcgcagcgc catgatggcg ttttagcgca tccatcagca
    37801 acgttgccag ccagtgtaaa cgcgccggag cttgttcatg attaagggcc gccagcagcg
    37861 aataccagtc gcccgatggc acgctatatg ccaacgcctg acacaatgtt tcacgagcct
    37921 gccagttatc tccctgaaac aacgccagtg ccgcgccagg cgaaccggcg cttaagcgca
    37981 atgcggcaag taatgcatcc tgtgacattg tcacttcgcg tgaaagccaa gtcacggcgt
    38041 actgttctgg aggcggcgca aggtaatgta accgacaacg actacgtaat gttgccagta
    38101 aacgttcagg ctcgcgggta gccaggaaaa accaggtttc tgctggtggc tcttcaagcg
    38161 ttttcagcaa tgcgttagcc gcggcgtcgg tcagtaaggc agcatcggtt acccagacaa
    38221 ctttcgcacc acctaggcgt gcgtgctcat tcagcttttc ggtgacctca cgtaccgcat
    38281 caatgcccag cgtatttttc cctttttcgg gagccagggt gtagtaatcg ggatgcgtgc
    38341 cagcctgcat caactgacat ccacgacagt gaccgcaact tttgtggccc tgcggttgtt
    38401 ggcagagtaa ataacggctc agggcgtaga ttaaagcatc atcgcccatg cccggtaacg
    38461 cctgaatgag tagcgcatgg tgacctcttc cggcctgata gctggctacc agtttttcga
    38521 aatcaggtcg taaccatgga taccatctca tgcgtccaac tccttcaccc agtgggtcac
    38581 ggtagtgcgg attgcatcca tcacggcctc cagcggctgg gtggcatcaa tggtatgaat
    38641 acttttatct tgtgcagcca gttccagata gcgggcgcgg gtgcgattaa agaaatcgaa
    38701 agactcttgc tcaatacgat ccagctcgcc gcgcgcacgc gcgcgtttta agccaacttc
    38761 cggggtaaca tcgagataga gcgttaagtc ggggcgaaaa tccccgagaa cagcatcacg
    38821 cagtgttgcc agcatatgtt ggtcaatacc acgtcctccg ccctgatacg cctgagtgga
    38881 gagatcgtgg cgatcgccaa tcacccaggt gccgttagcc agcgcaggtt tgatgaccgt
    38941 ttctaccagt tgaacgcgcg cggcataaaa catcagaact tcggctttat cggtaatgac
    39001 ttcatcgcct accgatttga tatccagcac caggcttctt aacttttcgg caagttgcgt
    39061 accgccaggt tcccgagtga aaaccatgtc gcggataccc agttgctcga gcgtctcaac
    39121 caccacatta cgcgcggtag ttttgcctgc gccttccagc ccctcaatga cgatatactt
    39181 actgcgcatt tttttcctta agcactttca gataatcctg cacagacttg ttatgactgg
    39241 caagattggt attaaacgtg tgaccacctt taccatcggc cacaaaatag agatacggcg
    39301 tttttgccgg atgcgcagca gccttcagcg aatccgcccc cggcgtagct atcgcacccg
    39361 gcggcaaacc ggtaatggta taggtgttat acgctgtcgg cgtttccagg tctgcacgag
    39421 aaagtttgcc attataacgc tctcccatcc cgtaaatcac ggttgggtcg gtctgcaggc
    39481 gcataccaat gcgtaaacgg ttgataaata ccgaggcaac ctgatcgcgt tcactggcaa
    39541 cggcggtttc tttttcgata attgatgcca tcgtccccag ctgattttta tctttataag
    39601 gcagaccgtc cgcacgccct tcccaggcgc tatcgaccgc tttcaccatt ttcttgtgag
    39661 ctcgcttgag taacgcgaca tcggtggtat tggcggtata catcaaggtg tctggccaga
    39721 accaaccttc aatccactcc gggttttcca gttcaagtgc ctgcgctacg gtggcgtact
    39781 tatcgtcact cagcgtgtgc ttgatatacg gagcctcacg caattgcttg aggtagtcgc
    39841 tcagacgcat cccttctacc agtcgcagcg ggaactgtgc ttctttaccg ctttccaaca
    39901 atttcagcat ctcgcgcacg gtcatctgcg gtgtaaagcg gtaagtcccg gctttaaagt
    39961 gagaaagatc cggttcgata cgcagcagcc attgaaaaac ccgtgggcga ttgatgatct
    40021 tatcagcata aagctgttca ccgagcgcca gacgtccggt ccctggcttc agggtaaata
    40081 tcgtctcttc tttgataagc aatttgctgt cggcaagatg gcgaaccttc cagacgccca
    40141 caccagcggc gatacccagt accaccagca ataacaagat aatcaataac acttttttca
    40201 tgactaattc gggcgctcac aaagtggggc taaatattca tataacgttg ctgacgaaaa
    40261 ggtgacatcg ccacaggcac gtacgggcat cactggcatt aacgcattac aaataaccat
    40321 ctcatctgcc tgcaacgcct cctccagcga ggcttgcact tcgacaagct gataagagga
    40381 ttgtgccagt aaacggatac agaattgtcg cataatgccg ttaacacctg cctgatccag
    40441 tcgcggcgta taaactacat tgcccttccg ccagaacaaa ttagccgcac agcattccgt
    40501 aacccaccct tcgctgtcaa ggaccagcgc ctcatcagcg tttgtctgct caagatgaga
    40561 gcgaatcaat acttgctcaa gccgattaag atgtttaata cctgcaagat gaggattgcg
    40621 ccccagccgc accgggctta gcgccaacgt catcccttcg ttacgcaaac ggtcgtaatg
    40681 tgcaggataa gccgtaacgg agagaatccg cgttgctggt ccgctgttca atgtgctgta
    40741 ccctcgcccg ccactaccgc gactgatcac gactttcagc acaccatttt gctgttctgc
    40801 tgccagcgtt ttcatctctt gttcaagctg aggccagaag tcactggaaa tcatcaaccg
    40861 ctgacaagca tcctgtagtc gctggatatg cgccgataac aaactgactt taccgtcgat
    40921 aactctggcg gtggtaaaac aaccatcacc aaactgcgtt gcccgatcgc ttactgccag
    40981 cgattcctgc ttataaccgt taattaagaa cataccggct ccttatcaga gaattgttag
    41041 tgtggcagca tgttcactac ggaacaagtc ggaataaaag ctaagaaaaa aggcccgcaa
    41101 gcggaccttt tatatgggtg gaaaatgaca acttagatct ttttaaagat caaagaacca
    41161 ttagtgccac cgaagccgaa ggagttacac agcgtgtatt ccattccgct gacctgacgt
    41221 gcttcatgcg gtacgaaatc cagatcgcaa ccttcatccg ggttatccag gttgatggtt
    41281 ggcggaacag cctgatcgcg cagcgccagg atggagtaga tagattctac tgcacccgcc
    41341 gcacctaaca ggtgaccggt catagatttc gtggagctta ccaacacacg gcttgcagct
    41401 tcaccgaaga tggttttcac cgcctgcgct tcagctttat cgccagccgg cgtagaagta
    41461 ccgtgcgcgt taacgtagcc aatctgactc gcttcaatgc ctgcatcacg cagagcattt
    41521 gccattgcca gagctgcgcc tgcgccattt tctggcggtg acgtcatatg ataagcatcg
    41581 ctgctcatac caaagccgac gagttcagcg taaattttcg caccgcgttt tttcgcgtgt
    41641 tcgtactctt caagtaccag cataccggca ccatcgccca gtacgaagcc atcacgctct
    41701 ttatcccacg ggcggctcgc cgcttgcggg ttatcattgc gggtagataa tgcacgtgcc
    41761 gcgccaaaac caccaacgcc cagcggcgta ctggctttct ctgcgccacc tgcaaccatc
    41821 acgtcagcat cgccatacgc gataatacgc gcagcatggc caatgttgtg cacgccggaa
    41881 gtacaggcgg tcgcgataga gatgcttggg ccacgcaggc catacatgat agtcagatga
    41941 cctgccacca tgttcacaat cgttgacgga acgaagaatg ggctgatctt acgtggacca
    42001 ccgttcatca gagatgtgtg gttttcttcg atcagtccga ggccgccaat cccggagcca
    42061 attgcggcac caatgcgggt tgcgttctct tccgttattt caaggccaga atcctgcatg
    42121 gcctgaacgc cagcgacaat tccatattga atgaaggcat ccatcttgcg ctgttctttg
    42181 cgcgagataa tgtcctcaca gttaaaatcc tttactaagc cagcaaattt cgttgcatag
    42241 gcgctagtat cgaaatggtc gattaggctg atgccactct gaccggcaag cagagctttc
    42301 caggtagact ctacggtatt gccgacagga gacaacatgc ccagtccggt cacaactaca
    42361 cgacgcttag acacgtttgt cctccaggga gggaaaaatg attctagtgg gacaaaaaga
    42421 taaaactcag gcggtcgaac gaccgcctgg agatgttcac ttacgcctgg tggccgttga
    42481 tgtaatcaat ggcagcctga acggtggtga ttttctcagc ttcttcgtcc ggaatctcag
    42541 tatcaaactc ttcttccaga gccattacca gctcaacggt gtcaagagaa tccgcgccta
    42601 ggtcttcaac gaaagaagca ttgttggtaa cttcttcctg cttaacgccc agctgttcgc
    42661 cgataatttt cttaacgcgt tcttcgatag tgctcatact cttaaatttc ctatcaaaac
    42721 tcgctttcgc gatggttttc gtagtgtata aaatgttgaa aaatttgcaa ctaaatcccg
    42781 gcaggtctta ccacgatttt acgttatttt gaggccttcc cccccaataa cgcaaatcat
    42841 tttgcactaa ttattactca taaccacgca gactgcgcgc aacatgagca ttttgtgcaa
    42901 atcgcggtca gaccatgtac atcccgccgt tcacatgcaa agtttccccc gtgatgtaag
    42961 ctgcttcgtc ggatgccagg aatgcaaccg cgttggcgat ttcctgtgcg ccgccgaggc
    43021 gacccgcagg aacctgcgcc aggatacccg cacgctggtc atcgctcagc gcacgtgtca
    43081 tgtccgtttc aataaagccc ggagcaacaa cgtttacagt aataccgcgt gacgcaactt
    43141 cgcgcgccag tgatttactg aagccgatca agcccgcttt cgccgcagcg tagttggcct
    43201 gaccgccatt tcccatggta ccaaccacag aaccgatagt gataatacga ccatgacgct
    43261 ttttcatcat agcgcgcatt accgcttttg acagacggaa aacagatgaa aggttagttt
    43321 cgataatatc gttccactct tcatctttca ttcgcattaa caggttatca cgagtgatac
    43381 cggcattatt gaccaggata tccacttcac caaattctgc gcgaattttt tccagaacag
    43441 attcgataga tgccgggtcg gtcacattca acatcagacc tttgccgttg gcacctaaat
    43501 aatcactgat cgcctgagcg ccattttcac tggtcgcagt gccaataact ttcgcgccac
    43561 gggctgcgag cgtttcagca attgcgcggc caattccgcg gcttgcaccg gttaccagtg
    43621 cgatttttcc ttcaaaattc atgattttcc tcttttaaag ctcgagcgcc gctgccatcg
    43681 ctgaaggttc gttcagcgcc gaggcggtca gggtgtcgac aatgcgtttc gtcaggccag
    43741 taagcacctt gcccgggccg acttcataga gatgttctac gccttgcgct gccatgtact
    43801 caacagactt cgtccactga accgggttat acaactgacg taccagtgcg tcacggatgg
    43861 catcaccatt ggtttcgcat ttcacatcaa cgttattcac aacagaaact gttggtgcgt
    43921 taaaggtgat tttcgctaat tctactgcca gtttgtcggc tgctggtttc atcagcgcac
    43981 agtgagacgg tacgctcact ggtaacagca gcgcgcgttt tgcgccagca gctttacagg
    44041 cagcgccagc acgctcaacc gcttctttat gaccggcaat aaccacctgt cccggagagt
    44101 taaagtttac cggagaaacg acctgacctt ctgcagcttc ttcacacgct ttcgcaatag
    44161 acgcatcatc cagaccgatg attgccgcca tagcgcccgt gccttccggt acggcttctt
    44221 gcatgaactt accgcgcatc tcaaccagac gcaccgcatc agcgaaatca atcacgccag
    44281 cgcaaaccag cgcggagtat tcccccaggc tgtgaccggc catcattgcc ggtgctttac
    44341 cgccctgctg ctgccatacg cgatacagcg caacagatgc agtcaacagc gctggctggg
    44401 tttgccaggt tttattcagt tcttcagctg gcccctgctg ggtcagcgcc cacaggtcgt
    44461 agcccagcgc cgcagaagct tcagcaaacg tttcttcgac aattggatag ctcgccgcca
    44521 tatcagccag cattccaacg gtttgagaac cctgtccagg gaacacaaat gcaaattgcg
    44581 tcatgtttta atccttatcc tagaaacgaa ccagcgcgga gccccaagtg aatccaccgc
    44641 caaaggcttc aagcagaacc aactgccccg gcttaatgcg cccgtcgcgt acagcttcat
    44701 ccagcgcgca cgggacagag gccgcagagg tattaccgtg gcgatccagc gtcaccacga
    44761 cattgtccat cgacataccg agttttttcg ccgttgcact gataatacgc aggttagcct
    44821 gatgcggaac cagccagtcc agttgagaac ggtcaagatt attcgccgcc agcgtctcat
    44881 caacgatgtg cgccagttcc gttaccgcaa ccttgaagac ttcgttgccc gccatcgtca
    44941 gatgaattga attctctgga ttcacgcggt cggcgtttgg cagcgtcagc aattcaccat
    45001 aactaccgtc ggcatgcaga tgggtggaaa tgattcccgg ctcttcagag gcagccagca
    45061 ccgcagcgcc cgcgccatcg ccaaaaataa taatagtccc acgatctgtt ggatcgcagg
    45121 tgcgcgccag tacatcggaa ccgacgacca gagcatactt caccgcccca gatttcacgt
    45181 attgatcggc tacgcttaat gcataggtga aacctgcgca ggctgctgca acgtcaaatg
    45241 ccgggcaacc tttaatgccc aacatgcttt gaatctgaca agctgcgctc gggaaagcgt
    45301 gcgtagcaga agtcgttgcc acaacgatca ggccaatctg gtctttctca atgcccgcca
    45361 tctcaattgc gcgtgtcgcc gcttcaaagc ccatggttga aacggtttcg tttggcgcgg
    45421 caatgtggcg ttcgcggata ccggtacgag tgacaatcca ctcgtcagag gtgtccacca
    45481 ttttttccaa atcggcgttt gtccgcactt gttcgggcag atagctgcca gtaccaataa
    45541 tcttcgtata catgtacgct cagtcacttt tcggttatat accgtcactt gcaaactgcg
    45601 agttcgctgg cagcgtcctg ctaccgcaga gttccgcttt tgccaccgtc cagcagctca
    45661 aaaccagctg ggtatacaga ttccaggcga gcggcaattc gctgaggaac ttgtcgctgc
    45721 accgcctgca ctgcctgttc aatcgcgacc gcaaaagctc gctgattggc tgcaccatga
    45781 ctttttatca ccgtgccgcg caatcctaac agacaggcgc cgttatactg gtcggggttg
    45841 aggtgactga atcgcctcgt caggctcttt tgtagccaac gctttaataa cagtagccac
    45901 cacgaccgtt ttttcccttc accctgagat ttcagcagag aaaggaacat cctgacaaca
    45961 ccttccatcg tctttaacgt gacatttcct gtaaagccgt cacaaaccag cacatctgtc
    46021 ttgccagtta acaactcatt ggcttcaaga tagccgatat aattgataga agggattgtt
    46081 ttaagcaccg ctgaggcatc ccgaatactg tcgagaccct ttacttcttc ttcaccaata
    46141 ttgagcaacg ccacgcgagg attgggaatt tccaccacct cttcagccag aactgagccc
    46201 ataatggcaa attgcaccaa cattgtgctg tcacaatcga cgttggcccc taaatcgagg
    46261 accaccgttt tgcccttttg ctgatgtggt aataccgtca ccagcgccgg acgctcaatc
    46321 ccctccaggg gcttgagtaa taattttgcc agccccatca gtgccccggt attaccggca
    46381 ctgacacagg cttgcgctcg accttctttc accagctcca gggccatacg cattgaactc
    46441 ccacgactgg cgcggatagc ttgcgaaggc cgggcatcac tggcgataac tgactgcgca
    46501 ggaataatct gcagacgcga acgttgttca aagtcagctt tagcaagtaa tggcgtgatg
    46561 gcgtcgggat tgccgactaa aagaagagtg agttgcgaat tagaattcag tgcctgcaat
    46621 gctgcaggca ctgtcacgga agggccaaaa tcccctccca tgacatctaa cgccagggtt
    46681 agacgtttca aggtatcgtc gccgcccggt ttggtctttc cccagttgcc tggggaaaat
    46741 cctcactaag cttcatcacg cagacgcgtg attacttagc gatgaccttg cggccgcggt
    46801 agtaaccgtc ggcagtgatg tggtgacgca ggtgtttttc accagaagtt ttgtctacag
    46861 acaggctggt gactgcggtc agcgcgtcat gggaacgacg catgccacgt ttggaacggg
    46921 ttggtttatt ctgttgtacg gccatggacc ttactcctca attacggttt atccccgatc
    46981 caacggggag caccaattac ttacgcttta agctggctaa tacggcaaat gggtttggct
    47041 tttgcacttc ttcaggcagt tcaccaaaga ccatgtccgc ttcggacact tcacagtgtt
    47101 cagaatcatg caccggaact accggcaagg cgaggatgat ttcatcttca accattgcaa
    47161 gcagatcgat ttcaccgaat tcgttaacct caatcggttc atacgcttcc ggcagtgctt
    47221 cagcctgttc gtctgaacgc acaggactaa aacaatacgt tgtgtagacc tgatgagtaa
    47281 acggcttccc gcaacgctga cactcgagcg ttaccgtcac cttcgcatcg ccgtttaaca
    47341 ctgcgagacg ttggttatcg atagcgaacg acatggagca ttccacatca ctgtccacac
    47401 tgactacgga ttcggcgacg cgctcaacct gatcaggggt atagataccc tggtaatcaa
    47461 ggcgtttttg agccgtacga accggatcga gagtcagggg taattttacc ttttgcatag
    47521 ggcgcgcata ttaactttgt aacgtcatag agtcaaagaa aaaggcagcc tgtggttgcc
    47581 ttttgccaat aattcgcaca cattgcgggt tactgcttta ttttcgttca gtgctgataa
    47641 cgaacatcaa cctgcaaaac gaagcgcata gtttaaaatg gctatcatca atacgctata
    47701 tctgatggaa aaaaatatgc ctaaacttat tttagcctcc acctcgccat ggcgtcgcgc
    47761 tcttctggaa aaactgcaaa tctcttttga atgtgcagca ccagaggtcg acgaaacccc
    47821 acgcagcgac gaatcaccgc gacagttggt gcttcgactg gcacaagaaa aagcgcaatc
    47881 tctggcgtca cgttatccgg atcatttaat tattggttca gaccaggtat gtgttcttga
    47941 tggcgaaatc accggtaaac cgttaacgga agaaaatgcc cgtctgcaat tacgcaaagc
    48001 cagcggcaat atcgtcacct tctatactgg actggcgttg tttaattcgg cgaatgggca
    48061 tctacaaaca gaagtggagc cttttgacgt ccatttccgt cacctaagcg aggcggagat
    48121 tgataattac gtgcgtaagg agcatcccct gcactgcgcg ggtagcttta agagtgaagg
    48181 atttggcatt acgctgtttg agcgtttaga gggacgtgac cctaacacgc tggttggttt
    48241 accgctaatc gcgctgtgtc agatgttacg tcgggaaggg aaaaacccgc tgatgggata
    48301 gttgtttgcg ccggatgcgg ttacgtcgca tccggcaatc ggtgcaggcg gcctgatgcg
    48361 gcgtaaacgc cttatcaggc caaccgccca ttatcaggct tatatgcttg tattagcgcg
    48421 cgttacgcag cttttgcaaa caacgcttca aaccttcatc catcggcgct tcgatacgca
    48481 tcacctcacc ggtccccgga tgggtaaact tcaacgctgc ggcgtgcagg aacagacgat
    48541 ttaatcccgt gcctgcttca gtgagctgtc tgtcaaattc acggtcaccg tagcgatcgt
    48601 caaaggcaat cggatgaccc gcatactgtg tatgcacacg gatctgatga gtacgtccgg
    48661 ttaccgggct acaacgcacc agggtggcaa atgcatagcg ttcttccact ttaaagcgtg
    48721 tttccgacgg tttgccttcc tgactgacac gcacgatacg ttcgccgctt tgcagaatat
    48781 ttttcaataa cggcgcttga acgctcttca catgcgactg ccactgaccg cgcaccagcg
    48841 ccaggtaatc tttttgcatt cctttttcac gtaattgctc atgcagagaa cgcaacgccg
    48901 agcgtttttt cgctaccagc aacacacctg aagtgtcccg gtcgagacga tgaaccagtt
    48961 caaggaaccg cgcttccggg cgcaacgccc gcaaaccttc gataacgccg aagcttaaac
    49021 cactgccgcc atgtaccgcc gtaccggaag gtttattcag caccaggata tgatcatctt
    49081 catataagat gacgtccgcc agcgccgcca ccttttgcag atgtggcgaa accgcctctt
    49141 cttcccgctc agcaacgcga accggtggga tacgcacctc atcacccgct tcgagtttat
    49201 attcaggctt aatacgtttt ttgttcaccc gcacttcacc tttacgcaaa atacggtaaa
    49261 tcatactttt tggtacgcct ttcaattggg tacgcaaaaa gttatcgata cgttgccccg
    49321 cttcgtcagc ggtgatagca acaattttta cggatggagt ctctgttttc atggtgggcg
    49381 attctaatta gccaacagga ttcgcgccac tcatttttct atgcttatat ttagttttgc
    49441 accttattac ttcactacgt gatcacttta ttgatggtta ttaaaccaat caccagcaag
    49501 aagtgaaaaa actgtgagta agcgggtgat aaatggtaaa agtcatcttg ctataacaag
    49561 gcttgcagtg gaataatgag gccgtttccg tgtccatcct tgttaaaaca agaaatttta
    49621 cggaataacc cattttgccc gaccgatcat ccacgcagca atggcgtaag acgtattgat
    49681 ctttcaggca gttagcgggc tgcgggttgc agtccttacc ggtagatgga aatatttctg
    49741 gagagtaata cccagtctgt ttctttgata attgcgctgt ttttccgcat gaaaaacggg
    49801 caaccgacac tctgcgcctc tttgagctga cgataaccgt gaggttggcg acgcgactag
    49861 acacgaggcc atcggttcac acccggaaag gcgttacttt gcccgcagct tagtcgtcaa
    49921 tgtaagaata atgagtaagt tacgatgaaa agaatgttaa tcaacgcaac tcagcaggaa
    49981 gagttgcgcg ttgcccttgt agatgggcag cgtctgtatg acctggatat cgaaagtcca
    50041 gggcacgagc agaaaaaggc aaacatctac aaaggtaaaa tcacccgcat tgaaccgagt
    50101 ctggaagctg cttttgttga ttacggcgct gaacgtcacg gtttcctccc actaaaagaa
    50161 attgcccgcg aatatttccc tgctaactac agtgctcatg gtcgtcccaa cattaaagat
    50221 gtgttgcgtg aaggtcagga agtcattgtt cagatcgata aagaagagcg cggcaacaaa
    50281 ggcgcggcat taaccacctt tatcagtctg gcgggtagct atctggttct gatgccaaat
    50341 aacccgcgcg caggtggcat ttctcgccgt atcgaaggcg atgaccgtac cgaattaaaa
    50401 gaagcactgg caagccttga actgccggaa ggcatggggc ttatcgtgcg caccgctggc
    50461 gtaggcaaat ctgctgaggc gctgcaatgg gatttaagct tccgcctgaa acactgggaa
    50521 gccatcaaaa aagccgctga aagccgcccg gctccgttcc tgattcatca ggagagcaac
    50581 gtaatcgttc gcgccttccg cgattattta cgtcaggata tcggcgaaat ccttatcgat
    50641 aacccgaaag tcctcgaact ggcacgtcag catatcgctg cattaggtcg cccggatttc
    50701 agcagcaaaa tcaaactgta caccggcgag atcccgctgt tcagccacta ccagatcgag
    50761 tcgcagatcg agtccgcctt ccagcgtgaa gtccgtctgc catccggtgg ttccattgtt
    50821 atcgacagca ccgaagcgtt aacagccatc gacatcaact ccgcacgcgc gacccgcggc
    50881 ggcgatatcg aagaaactgc gtttaacacc aacctcgaag ccgccgatga gattgctcgt
    50941 cagctgcgcc tgcgtgacct cggcggcctg attgttatcg acttcatcga catgacgcca
    51001 gtacgccacc agcgtgcggt agaaaaccgt ctgcgtgaag cggtgcgtca ggaccgtgcg
    51061 cgtattcaaa tcagccatat ttctcgcttt ggcctgctgg aaatgtcccg ccagcgcctg
    51121 agcccatcac tgggtgaatc cagccatcac gtctgcccgc gctgctccgg taccggtacc
    51181 gtgcgtgaca acgaatcgct gtcgctctct attctgcgct tgatcgaaga agaagcgctg
    51241 aaagagaaca cccaggaagt tcacgccatt gttcctgtgc caatcgcttc ttatctgctg
    51301 aatgaaaaac gttctgcggt gaatgcgatt gaaacgcgtc aggacggtgt tcgctgcgtg
    51361 attgtgccaa acgatcagat ggaaaccccg cactaccacg tgttgcgcgt gcgcaaaggg
    51421 gaagaaacgc cgaccttaag ctacatgctg ccgaagctgc atgaagaagc gatggcgctg
    51481 ccgtctgaag aagagttcgc tgaacgtaag cgtcctgaac aacctgcact ggcaaccttt
    51541 gccatgccgg atgtgccgcc agcgccaacc ccagctgaac ctgccgcgac tgtcgtagcc
    51601 ccagcaccga aagctgcaac ggcaacacca gcagctcctg cacaacctgg gctgttaagc
    51661 cgcttcttcg gcgcactgaa agcgctgttc agcggtggtg aagaagccaa accgaccgag
    51721 caaccaacac cgaaagcaga agcgaaaccg gaacgtcaac aggatcgtcg caaacctcgt
    51781 cagagcaacc gccgtgaccg taatgagcgc cgcgacaccc gtagtgaacg tactgaaggc
    51841 agcgataatc gcgaagaaaa ccgtcgtaat cgtcgccagg cacagcagca aactgccgag
    51901 acgcgtgaga gccgtcagca ggctgaggta acggaaaaag cacgtaccac cgacgagcag
    51961 caagcgccgc gtcgtgaacg tagccgccgc cgtaacgatg ataaacgtca ggcgcaacaa
    52021 gaagcgaagg cgctgaatgt tgaagagcaa tctgttcagg aaaccgaaca ggaagaacgt
    52081 gtacgtccgg ttcagccgcg tcgtaaacag cgtcagctca atcagaaagt gcgttacgag
    52141 caaagcgtag ccgaagaagc ggtagtcgca ccggtggttg aagaaactgc cgctgccgaa
    52201 ccaattgttc aggaagctcc agctccacgc acagaactgg tgaaagtccc gctgccagtc
    52261 gtagcgcaaa ctgcaccaga acagcaagaa gagaacaatg ctgataaccg tgacaacggt
    52321 ggcatgccgc gtcgttctcg ccgctcgcct cgtcacctgc gcgtgagtgg tcagcgtcgt
    52381 cgtcgctatc gtgacgagcg ttatccaacc cagtcgccaa tgccgttgac cgtagcgtgc
    52441 gcgtctccgg aactggcctc tggcaaagtc tggatccgct atccaattgt acgtccgcaa
    52501 gatgtacaag ttgaagagca gcgcgaacag gaagaagtac aagtgcagcc gatggtgact
    52561 gaggtccctg tcgccgccgc tgtcgaacct gttgttagcg cgccagttgt tgaagaaatg
    52621 gctgaagtcg tagaagcccc cgtaccggtt gccgaaccac aaccggaagt ggttgaaacg
    52681 acgcatcctg aagtgattgc cgccgcggta actgaacagc cgcaggtgat taccgagtct
    52741 gatgttgccg tagcccagga agtcgcagaa cacgcagaac cggtggttga accgcaggaa
    52801 gagacggcag atattgaaga agttgccgaa actgctgagg ttgtggttgc tgaacctgaa
    52861 gttgttgctc aacctgccgc gccagtcgtc gctgaagtcg cagcagaagt tgaaacggta
    52921 gccgcggttg aaccagagat caccgttgag cataaccacg ctaccgcgcc aatgacgcgc
    52981 gctccggcac cggaatatgt tccggaggca ccgcgtcaca gtgactggca gcgccctact
    53041 tttgccttcg aaggtaaagg tgccgcaggt ggtcatacgg caacgcatca tgcctctgcc
    53101 gctcctgcgc gtccgcaacc tgttgagtaa taattagctc aaagtaatca agccctggta
    53161 actgccaggg cttttttatt tcatgtttga atcaacatat taaaattcat caaatggcat
    53221 ccttgctaac caacaatgca aaataggcaa aaaaaatgcc cgccccggcg atcccagagc
    53281 gggcagacaa aacataccca gtttcatgat atgttttaaa gcgagcttat ttgttgagct
    53341 ggaagagcga caatccctgc atatcggtaa atgctttata cgatgcctgc aatgccgttt
    53401 gctgcatgat gtaagatgaa atagttgcat tccagtccac atcaaccaga tcgctcatct
    53461 gctgcgtttg ccctaaagcg cgatcgctac ctaaggaatc cagcgactcc agtttgttca
    53521 gctgcgtgcc taattccgcg cgaacggtaa ggacattatt cagcgagttt ttcagtccac
    53581 ggttggtttt atccaatgcc acagcggcga tttctttatc cgcttcgcta tccgcgaccg
    53641 gcgttttcag ggctgcgatg gcgctatcca gcatggcaaa aagattggtt tcagaagcgc
    53701 taccgtctgg ttccgctacc gcgttactgg taatactgtc gaaaattttg tcgcccgtat
    53761 gtcctatcac catcgaacgc gaagcatcga cctgttgctt aatactttct gttccgccga
    53821 cataatcacc gttagcctca ctaaacggcg cagtctctgt tttataaccg gcaaaaatgt
    53881 agcgcccatt accgtcagtg gtgttcgcca gattcagcaa ctggtcacga agcccctgaa
    53941 tatccgtagc cagcgaggcc cggtcatcgt cactcaaggt gccattgctg gcgtagacaa
    54001 ttttttcctg agcattctgg atagcagtgg tgacctggct aagtacactc tcttccagtg
    54061 acactttttg agtggcgaaa gtacgtgcca gcgtgtactg gctgttttgc gcctgtgcct
    54121 gggagagaac aacagcttgt gatgcagcaa tgggatcgtc tgaagggtta acgactcgct
    54181 tacccgtcga catctgctcg ccgtacttca tccattctgc ctgagaattg gtgatgccgc
    54241 gcatgttttg ctggtacatc atctgggtac tgaaacgcat tttatctccc cttagcgaat
    54301 gttaatcaga gcatcaaaaa tcgtgtttgc cgtctgcaga acctgcgcat tcgccaggta
    54361 atactgctga aaacgttgca gatttccgta ctcctcatcg agattgacac cggaaatcga
    54421 ctgctgctga ttggaaagct gcgtcaccac attaccttgc gtggtgctac tggttttcaa
    54481 catcgcggtt ttattaccga tatcactcac taacgaggca taagcgtcgt taaaggattt
    54541 cgcaccgccc accgttttac tgttgctttg cagatccagc agggcctgac cgttgcggtt
    54601 gtcgctatca cccgcatctt cttcgctcgc catcgcgatt ttcgcttcgt cggtgattaa
    54661 tacatccatg ttgacgatgg cgtcacttac tggtttcagc gtgaagctgt cgttaacggc
    54721 aggcgttcct gtaaacgtca actccagacc atcaaatgcc actttaccgt tggcatctgg
    54781 tgtcgccgta aaagtggtat tgctggcaag gcgggtgacc tgccactgat tattatcgaa
    54841 cgagattttg taatctgtcg ccagtaccac ggaggcatca gttaccgtgg caccgatcgc
    54901 aacgtcacct ttgtttttcg tgttttgcag aaccgcgggc ttaccgatag taaagaaatc
    54961 ttcaccagca tcgccgttag catcaaatcc ggctttgtgt tggctgttga aagcctcggc
    55021 aaatgccagc gccagttgtc caagcgtatt acgcgtctgg tccagatcct gagaacggaa
    55081 tgtcagaatg ccgcccagcg acccggtatt cagtaatttc tccgggatct caatattgcc
    55141 tgccgtccca tcaacgtaag cgacagtcgt acgagaaggg tcggcgctgg aaggaactgc
    55201 cgccagttgc cgcgccgtac ttccctgaac cagtgagtaa ccattggcat cgtgatgtta
    55261 taagtaccgc catcctgaac gctgacttct acgccaacaa tctggtttaa ttcgctcacc
    55321 agttgatcgc gttgatccag cagattgtta ggtgacgccc ctgcccccac gcctgtcagg
    55381 cgcgagattt gatcgttcag gctggcaatt tgtttagcgt aattgttgat ctgatcaacg
    55441 ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc gcagatattg atcggtggtt
    55501 ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg cctggcgcgc cgccgggtct
    55561 tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat cctgcatctg tgttgccagc
    55621 gaagaggtac tggtggagag catattgtcg attttcgaca tctgctcata gcgggcggtc
    55681 aggccgctac tttgcgtctg cgcagcacgt aactggttgg taataaacgt atcatactca
    55741 cgctgcacac cagaaacgta gacgccattg ccaacccagc cgccagcgcc caacgtgcta
    55801 ttggcctgcg ccataatagt ggtttggcgg gtatatccgg caacgttata gctggagata
    55861 ttattacttg ccgtatttaa cgccgcctgg gccgcgttca gtccgctcat ggcgttattg
    55921 atcaagctgg acatggaggt tccttataag ccttcaatta cagagtatta tcggcagcga
    55981 cccgccggac ttgagttatt cagaacagat tatcaatgtt catgctgtag gttttgctca
    56041 ccttgtcgct tatcgatttc atctgctgga tcatgttggt gagtttacgg gcatagtgag
    56101 gatcggtagc atagcccgcg tcctgtagga cctgcgcccc ctgttccgca ctcgcggcgg
    56161 tcgtcacggc ggcgtagcgc gggttacgtg ttaacagccc aacgtaatcc gacaatgctt
    56221 ccagatacga gctgtagacc cgaaacttcg cttttacttt cttcgcttcg ccgttttcat
    56281 attcagtcgt ggtgatttca gtgacttgcc ctttccagtt gccagaggct ttgacaccaa
    56341 acaggttata gcttggctcg ccgttttcgt ggcggatttg ccgttgcccc caaccagatt
    56401 caagcgccgc ctgagcgagg atcaaatgat gtggcacacc gctttgctgg cttgccagtt
    56461 gggcgggcag cgagagttgc gcgaggaatg ctctactgtc acccggcagc gaatcatcgt
    56521 agttacgtgg tacggc
//
LOCUS       DBKEKI010000010        54636 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.010, whole genome shotgun sequence.
ACCESSION   DBKEKI010000010 DBKEKI010000000
VERSION     DBKEKI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.010"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            546..1835
                     /gene="sdaC"
                     /locus_tag="ACUQPX_000585"
     CDS             546..1835
                     /gene="sdaC"
                     /locus_tag="ACUQPX_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HHV9055381.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1893..3260
                     /gene="sdaB"
                     /locus_tag="ACUQPX_000586"
     CDS             1893..3260
                     /gene="sdaB"
                     /locus_tag="ACUQPX_000586"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HHV9055382.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3371..4126
                     /gene="xni"
                     /locus_tag="ACUQPX_000587"
     CDS             3371..4126
                     /gene="xni"
                     /locus_tag="ACUQPX_000587"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HHV9055383.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4179..5327)
                     /gene="fucO"
                     /locus_tag="ACUQPX_000588"
     CDS             complement(4179..5327)
                     /gene="fucO"
                     /locus_tag="ACUQPX_000588"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HHV9055384.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5355..6002)
                     /gene="fucA"
                     /locus_tag="ACUQPX_000589"
     CDS             complement(5355..6002)
                     /gene="fucA"
                     /locus_tag="ACUQPX_000589"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HHV9055385.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6549..7865
                     /gene="fucP"
                     /locus_tag="ACUQPX_000590"
     CDS             6549..7865
                     /gene="fucP"
                     /locus_tag="ACUQPX_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HHV9055386.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7898..9673
                     /gene="fucI"
                     /locus_tag="ACUQPX_000591"
                     /pseudo
     CDS             7898..9673
                     /gene="fucI"
                     /locus_tag="ACUQPX_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            9782..11200
                     /gene="fucK"
                     /locus_tag="ACUQPX_000592"
     CDS             9782..11200
                     /gene="fucK"
                     /locus_tag="ACUQPX_000592"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HHV9055387.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVNKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTSELDSQAWLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     YELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            11202..11624
                     /gene="fucU"
                     /locus_tag="ACUQPX_000593"
     CDS             11202..11624
                     /gene="fucU"
                     /locus_tag="ACUQPX_000593"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HHV9055388.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11682..12413
                     /gene="fucR"
                     /locus_tag="ACUQPX_000594"
     CDS             11682..12413
                     /gene="fucR"
                     /locus_tag="ACUQPX_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HHV9055389.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="ACUQPX_000595"
     CDS             complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="ACUQPX_000595"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HHV9055390.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13550..13945)
                     /locus_tag="ACUQPX_000596"
     CDS             complement(13550..13945)
                     /locus_tag="ACUQPX_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HHV9055391.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13964..14881)
                     /gene="gcvA"
                     /locus_tag="ACUQPX_000597"
     CDS             complement(13964..14881)
                     /gene="gcvA"
                     /locus_tag="ACUQPX_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HHV9055392.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15232..15459)
                     /locus_tag="ACUQPX_000598"
     CDS             complement(15232..15459)
                     /locus_tag="ACUQPX_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HHV9055393.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15651..16856
                     /gene="csdA"
                     /locus_tag="ACUQPX_000599"
     CDS             15651..16856
                     /gene="csdA"
                     /locus_tag="ACUQPX_000599"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HHV9055394.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16856..17299
                     /gene="csdE"
                     /locus_tag="ACUQPX_000600"
     CDS             16856..17299
                     /gene="csdE"
                     /locus_tag="ACUQPX_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HHV9055395.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="ACUQPX_000601"
     CDS             complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="ACUQPX_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HHV9055396.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="ACUQPX_000602"
     CDS             complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="ACUQPX_000602"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HHV9055397.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19701..19777
                     /locus_tag="ACUQPX_000603"
     tRNA            19701..19777
                     /locus_tag="ACUQPX_000603"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19735..19737,aa:Met,seq:cat)
     gene            19811..19887
                     /locus_tag="ACUQPX_000604"
     tRNA            19811..19887
                     /locus_tag="ACUQPX_000604"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19845..19847,aa:Met,seq:cat)
     gene            complement(19961..21214)
                     /gene="amiC"
                     /locus_tag="ACUQPX_000605"
     CDS             complement(19961..21214)
                     /gene="amiC"
                     /locus_tag="ACUQPX_000605"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HHV9055398.1"
                     /translation="MSGSNTAISRRHLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21446..22777
                     /gene="argA"
                     /locus_tag="ACUQPX_000606"
     CDS             21446..22777
                     /gene="argA"
                     /locus_tag="ACUQPX_000606"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - L-glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HHV9055399.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAENMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22839..24665)
                     /gene="recD"
                     /locus_tag="ACUQPX_000607"
     CDS             complement(22839..24665)
                     /gene="recD"
                     /locus_tag="ACUQPX_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HHV9055400.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMFEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24665..28207)
                     /gene="recB"
                     /locus_tag="ACUQPX_000608"
     CDS             complement(24665..28207)
                     /gene="recB"
                     /locus_tag="ACUQPX_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HHV9055401.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEE"
     gene            complement(28200..31088)
                     /gene="ptrA"
                     /locus_tag="ACUQPX_000609"
     CDS             complement(28200..31088)
                     /gene="ptrA"
                     /locus_tag="ACUQPX_000609"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HHV9055402.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKDGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AIITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31264..34632)
                     /gene="recC"
                     /locus_tag="ACUQPX_000610"
     CDS             complement(31264..34632)
                     /gene="recC"
                     /locus_tag="ACUQPX_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HHV9055403.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKTLVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34645..34968)
                     /locus_tag="ACUQPX_000611"
     CDS             complement(34645..34968)
                     /locus_tag="ACUQPX_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HHV9055404.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(34953..35360)
                     /locus_tag="ACUQPX_000612"
     CDS             complement(34953..35360)
                     /locus_tag="ACUQPX_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HHV9055405.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHCWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35357..35920)
                     /locus_tag="ACUQPX_000613"
     CDS             complement(35357..35920)
                     /locus_tag="ACUQPX_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HHV9055406.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35911..36381)
                     /locus_tag="ACUQPX_000614"
     CDS             complement(35911..36381)
                     /locus_tag="ACUQPX_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HHV9055407.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLYQQGETEGCL"
     gene            complement(36565..37359)
                     /gene="thyA"
                     /locus_tag="ACUQPX_000615"
     CDS             complement(36565..37359)
                     /gene="thyA"
                     /locus_tag="ACUQPX_000615"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HHV9055408.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(37366..38241)
                     /gene="lgt"
                     /locus_tag="ACUQPX_000616"
     CDS             complement(37366..38241)
                     /gene="lgt"
                     /locus_tag="ACUQPX_000616"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HHV9055409.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38392..40638)
                     /gene="ptsP"
                     /locus_tag="ACUQPX_000617"
     CDS             complement(38392..40638)
                     /gene="ptsP"
                     /locus_tag="ACUQPX_000617"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HHV9055410.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40651..41181)
                     /gene="rppH"
                     /locus_tag="ACUQPX_000618"
     CDS             complement(40651..41181)
                     /gene="rppH"
                     /locus_tag="ACUQPX_000618"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HHV9055411.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(41534..41680)
                     /gene="ygdT"
                     /locus_tag="ACUQPX_000619"
     CDS             complement(41534..41680)
                     /gene="ygdT"
                     /locus_tag="ACUQPX_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HHV9055412.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            41866..42555
                     /gene="mutH"
                     /locus_tag="ACUQPX_000620"
     CDS             41866..42555
                     /gene="mutH"
                     /locus_tag="ACUQPX_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HHV9055413.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42624..43337
                     /locus_tag="ACUQPX_000621"
     CDS             42624..43337
                     /locus_tag="ACUQPX_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HHV9055414.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43475..43693
                     /gene="ygdR"
                     /locus_tag="ACUQPX_000622"
     CDS             43475..43693
                     /gene="ygdR"
                     /locus_tag="ACUQPX_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HHV9055415.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43794..44834
                     /locus_tag="ACUQPX_000623"
     CDS             43794..44834
                     /locus_tag="ACUQPX_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HHV9055416.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44867..46060)
                     /gene="lplT"
                     /locus_tag="ACUQPX_000624"
     CDS             complement(44867..46060)
                     /gene="lplT"
                     /locus_tag="ACUQPX_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HHV9055417.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(46053..48212)
                     /gene="aas"
                     /locus_tag="ACUQPX_000625"
     CDS             complement(46053..48212)
                     /gene="aas"
                     /locus_tag="ACUQPX_000625"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HHV9055418.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSCQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRVKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48797..49828
                     /gene="galR"
                     /locus_tag="ACUQPX_000626"
     CDS             48797..49828
                     /gene="galR"
                     /locus_tag="ACUQPX_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HHV9055419.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49835..51097)
                     /gene="lysA"
                     /locus_tag="ACUQPX_000627"
     CDS             complement(49835..51097)
                     /gene="lysA"
                     /locus_tag="ACUQPX_000627"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HHV9055420.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51219..52154
                     /gene="lysR"
                     /locus_tag="ACUQPX_000628"
     CDS             51219..52154
                     /gene="lysR"
                     /locus_tag="ACUQPX_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HHV9055421.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52141..52833)
                     /gene="ygeA"
                     /locus_tag="ACUQPX_000629"
     CDS             complement(52141..52833)
                     /gene="ygeA"
                     /locus_tag="ACUQPX_000629"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HHV9055422.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52962..54380)
                     /gene="araE"
                     /locus_tag="ACUQPX_000630"
     CDS             complement(52962..54380)
                     /gene="araE"
                     /locus_tag="ACUQPX_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HHV9055423.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        13361 a        14766 c        13478 g        13031 t
ORIGIN      
        1 ccggtgacgt ttgttgccgg gttttctcgt ttttggtcac ttactcatca actcatttca
       61 tttgttatat gaatgtttct taccaccctc acgcgacaaa tatcatcaca gttaatatgt
      121 catacaattt atgttgcaac gcaaacgttt ccctattttc ataaaccgta ttttatcgct
      181 atgaaaaaga aattatcgcc atgattaact aaaagtattg attttttcag ttcaacctac
      241 atatattgcg cgccccggaa gaagtcagat gtcgtttaat gggcaaatat tgcccttaaa
      301 ttctctttta cttttgattt acagagtaaa gcgttgggat aatctatctt ccaagtagat
      361 tattgtattt gggatcaaga tcactgatag atacataact tgtgtgtatc tttccgccct
      421 caaattatta cggcggtaaa tgattaagcc atcgccgata gacagatttc atttttacgg
      481 tcaggcacct tcccgggctg aactggctaa aagctgaatt atttgcattc ctccaggaga
      541 aatagatgga aacgactcaa accagcacga ttgcgtcgaa agactctcgt agtgcctggc
      601 gcaagacaga caccatgtgg atgctgggcc tttacggcac ggcaatcggc gcgggcgtgc
      661 tgttcctgcc aatcaacgcc ggtgttggcg gtatgatccc gctgatcatc atggctatcc
      721 ttgcgttccc gatgacgttt tttgctcacc gcggcctgac tcgcttcgta ctgtctggta
      781 aaaacccggg cgaagacatc accgaggttg tagaagaaca ctttggtatt ggcgcaggta
      841 aactgattac cctgctctac ttcttcgcta tctacccgat cctgctggtt tatagcgtgg
      901 caatcaccaa taccgttgaa agcttcatgt ctcaccagct gggtatgacg ccaccgccgc
      961 gtgcgattct gtcgctgatc ctgatcgtgg gtatgatgac catcgttcgc tttggtgagc
     1021 agatgatcgt taaagcgatg agtattctgg tattcccgtt tgttggcgta ctgatgctgc
     1081 tggctctgta cctgatcccg cagtggaacg gcgcagcact ggaaacgctg tctctggaca
     1141 ctgcatctgc aaccggaaac ggtctgtgga tgaccctgtg gctggcaatt ccggtaatgg
     1201 tgttctcgtt caaccactct ccgatcatct cttctttcgc cgttgcgaag cgtgaagagt
     1261 acggcgatat ggcagaacag aaatgctcga agatcctggc attcgcacac atcatgatgg
     1321 tgctgaccgt aatgttcttc gtcttcagct gcgtactgag cctgactccg gcagacctgg
     1381 ctgcggctaa agagcagaac atctcgattc tgtcttacct ggctaaccac tttaacgcac
     1441 cggttatcgc gtggatggct ccgattatcg cgattatcgc tatcaccaaa tccttcctcg
     1501 gccactacct gggcgcacgt gaaggcttca acggtatggt gattaaatct ctgcgtggta
     1561 aaggtaagtc tatcgaaatc aacaagctga accgtatcac tgcgctgttc atgctggtaa
     1621 cgacctggat tgttgccacc ctgaacccga gcatcctggg tatgattgaa accctgggcg
     1681 gcccaatcat cgcgatgatc ctgttcctga tgccgatgta cgcaattcag aaagtaccgg
     1741 caatgcgtaa gtacagcggt cacatcagca acgtattcgt tgtcgtgatg ggtctgattg
     1801 caatctccgc aatcttctac tctctgttca gctaagtcct ttcgcgccgc tttcgggcgg
     1861 cgcttcctcc gttttaacgc gatgtatttc ctatgattag cgtattcgat attttcaaaa
     1921 tcggcattgg cccttccagt tctcataccg ttggaccaat gaaagcgggt aaacaattta
     1981 ccgacgatct gattgcccgt aacctgctta aagacgtgac ccgcgtggtg gttgacgtgt
     2041 acggctcgct ctctctgact ggtaaaggcc accacacaga tatcgccatt attatgggcc
     2101 tagcgggtaa cctgccggat accgtggata tcgattccat ccccggtttt attcaggatg
     2161 tgaatactca tggtcgcctg atgctggcaa acggtcagca tgaagtggag ttcccggttg
     2221 atcagtgcat gaacttccat gcagacaacc tttctctgca tgaaaacggt atgcgcatta
     2281 ccgcgctggc gggcgataaa gtcgtttaca gccagactta ctactctatt ggcggtggct
     2341 ttatcgttga tgaagagcat tttggccagc aggatagcgc accggttgaa gttccttatc
     2401 cgtacagttc agcagccgat ctgcaaaaac attgtcagga aaccgggctg tcactctctg
     2461 gcctgatgat gaaaaacgag ctggcgctgc acagtaaaga agagctggaa cagcacctgg
     2521 cgaacgtctg ggaagtcatg cgtggcggta ttgagcgcgg tatttccacc gaaggcgtgt
     2581 tgcctggcaa actgcgcgtt ccacgccgtg ctgcggcact acgccggatg ctggtcagcc
     2641 aggataaaac caccactgac ccgatggcgg ttgttgactg gatcaacatg tttgcactgg
     2701 cagtgaacga agagaacgct gctggcggtc gcgtggtgac tgcgccgact aacggtgcgt
     2761 gcgggattat cccggctgtg ctggcgtact acgataagtt tatccgcgaa gtgaacgcta
     2821 actcactggc tcgttacctg ctggtagcca gcgccattgg ttctctttat aagatgaacg
     2881 cgtcgatttc tggtgccgaa gtgggttgcc agggtgaagt tggcgtggcg tgctctatgg
     2941 cggcagctgg tctggcagaa ctgttaggcg caagcccggc gcaggtttgc atcgcagcgg
     3001 aaatcgcgat ggagcacaac ctcggtctga cttgtgaccc ggtcgcgggg caggttcagg
     3061 taccgtgcat tgagcgtaac gccattgcag cagtgaaagc ggtgaacgcc gcgcgtatgg
     3121 cactgcgccg taccagcgaa ccgcgcgtct gcctcgataa agttatcgaa accatgtacg
     3181 aaacaggtaa agacatgaac gccaagtacc gcgaaacctc tcgcggcggc ctggcgatga
     3241 agatcgttgc ctgcgattaa tctctcccag aggcctcgtt ttgcgaggcc tcttcctgtt
     3301 ttctcaacca gccatagcct gttccggcat cgaatgttac ccttatcgcc tgatctttaa
     3361 gggggttatc gtggctgttc atttgcttat tgtcgatgca ctgaatctta tccgtcgcat
     3421 tcatgccgtt cagggttcgc cctgtgtcga aacctgccag catgcgctcg atcagctcat
     3481 tatgcacagc cagccaaccc acgcggtcgc cgtttttgat gatgaaaacc gcagtagcgg
     3541 ctggcgtcat cagcgtttac cagattacaa agcggatcga ccgccaatgc cggaagagtt
     3601 gcacgacgag atgcctgcat tacgcgccgc ctttgagcaa cgcggcgtcc cgtgctggtc
     3661 agccagcggc aacgaagccg atgacttagc cgccacgctg gcggtcaaag tgacacaggc
     3721 cgggcatcag gcaacgattg tttcgacaga taaaggctac tgtcagttac tttcaccgac
     3781 attacgtatt cgtgattact tccagaaacg ttggctggat gcgccattta tcgataaaga
     3841 atttggcgtt caaccgcagc agttgcccga ttactgggga cttgcgggga tcagcagttc
     3901 aaaggtaccg ggtgttgcgg gaatcggacc aaaaagcgcc acgcagctgc tggtcgagtt
     3961 tcagagtctg gaagggatat atgagaatct ggatgcggtt gccgaaaagt ggcgcaaaaa
     4021 attagaaacc cataaagaga tggcgtttct gtgccgcgat attgcccgct tacaaaccga
     4081 tttgcatatc gacggcaatt tacagcaatt gcggttggta cggtaacggc gagccggata
     4141 cgccgcaaat cgtatccggc attatcacat cagcgcattt accaggcggt atggtaaagc
     4201 tctacaatat cctcaagcgt tgcttcacgc gggttgccac cggtacaaac atcatccagt
     4261 gccgcctgag ccagtgccga aatgtcttcc ttgcgtacac caacatcacg caaatgtggc
     4321 ggaataccga catcacggtt gagggcaaac accgcttcaa cagcggcatt acgcgcccct
     4381 tccaggctca taccttccac tttcacgccc ataacgcgcg cgatatcgcg gtacttctca
     4441 ccggtaaagt cagcgttata gcgcatgaca tgcggtaaca ggatggcgtt cgcaacaccg
     4501 tgtggagtgt tataaaacgc gcccagtgga tgcgccatac catgcaccaa ccctaaccca
     4561 acattcgaga agcccatacc cgcaacatac tgcccgagcg ccatttcttc tccggcatcc
     4621 ttatcaccag caaccgatcc tcgcagcgcc ccagcaatga tttcaatcgc tttaatgtgc
     4681 agtgcatcgg ttagtgccca cgcgccacgg gtaatatacc cctcaatagc atgagtgagc
     4741 gcatcgacac ccgtcgcggc tttcagcgcc ggaggcatac catccatcat gtcagcatca
     4801 ataaacgcca cctgcgggat atcatgcgga tcaacgcaaa caaacttgcg ccgtttttct
     4861 tcgtcagtga tcacgtagtt aatggtcact tctgccgcag tacctgctgt ggtggggatt
     4921 gccagaatcg gtacactggg tttattggtc ggggaaagcc cttccaggct acgcacatcg
     4981 gcaaactccg ggttgttgct gataatgcca attgctttac aagtatcctg tggagaacca
     5041 ccaccaatag cgatcaggta atccgcgccg ctattctgga atacaccgag cccttctttg
     5101 acgacagtaa ttgttgggtt gggcactacg ccgtcgtaaa tcgcccatgc cagccctgca
     5161 gcatccatct tatcggtcac tttcgccacc acgccgcatt gcaccagcgt tttatcggtg
     5221 acgatcagcg ccttctgata accacggcgt ttcacctcat cggttaaagc cccaacagca
     5281 ccccgaccaa accatgccgt ttcgttcaga atcattctgt tagccatcat ccttctcctt
     5341 gttgctttac gaaattactc ttcaatccgt aacccatagg ttttgaattt ctccagcact
     5401 acggcaatct cttcatcgct cagcactggc accgggtccg taatcgccag ggtcgtcagg
     5461 taaagttgcg ccagcacttc aacttcatgc gccagccata acgctttttc cagattcacc
     5521 tcacaagcga taagcccatg atgttgtaac aaagttgcct tacgattttt gagagccagc
     5581 gcaacatgtt cagaaagttc gcgtgttcca aaggtcgcat aaggcgcgca aggaatagaa
     5641 ttaccgccag ccgccgcaat catgtagtga atagcgggga tcgatcggtt aagaatggaa
     5701 actgccgtgc aatgaacggc atgattgtga acaaccgcgt tggcatccgg tctgctttga
     5761 taggctgcca tatggaaacg ccattcgctt gaggggagct ttccttcctc atgtttaccg
     5821 ttgccatcaa taaagacaat atgcgactcc gtcagttttt catatggaat gcctgtaggc
     5881 gtaatcagca tcccatcctg ataacgtaca ctgacgttcc ccgctgtccc ctggttcagt
     5941 cccaggcggg tcatttccag gcaagtgtca ataatctgac gagcaagttt atttcgttcc
     6001 attagctacc tctctctgat tcaaaacagg gcaataatgt tgttcctttc acactattga
     6061 attagccgtt taattaccca ccatcttctt cctgattaac aagaaagaaa ttcacaagct
     6121 tatattttgt gacctagctc aactaatcac agtaaataac tgccagttct ctttttataa
     6181 ccccgttaaa aatgaccgct cttaaaaata tttatcaaaa cggtcatttt tccatttccc
     6241 ctcgccagaa atgcccgttt tcggcacaaa caattaatac ggtcatctgg tttgtgtttt
     6301 ttatgattta ttttctgaaa cggacatgaa atttcgatta ttaaagtgat ggtagtcaca
     6361 taaagtcacc ttccagctaa taagtgtgac cgccgtcata ttacagagcg ttttttattt
     6421 gaaaatgaat ccatgagttc atttcagaca ggcaaatatt caccgatatg aagcccgaac
     6481 tcgctggttt tgcacttttg aaaacataac cgattacgtg cttaagcttc tgaacctaag
     6541 aggatgctat gggaaacaca tcaatacaaa cgcagagtta ccgtgcggta gataaagatg
     6601 cagggcaaag cagaagttac attattccat tcgcgctgct gtgctcactg ttttttcttt
     6661 gggcggtagc caataacctt aacgacattt tattacctca attccagcag gcttttacgc
     6721 tgacaaattt ccaggctggc ctgatccaat cggcctttta ctttggttat ttcattatcc
     6781 caatccctgc tgggatattg atgaaaaaac tcagttataa agcagggatt attaccggat
     6841 tatttttata tgccttgggt gctgcattat tctggcccgc tgcagaaata atgaactaca
     6901 ccttattttt agttggccta tttattattg cagccggatt aggttgtctg gaaactgccg
     6961 caaacccttt tgttacggta ttagggccgg aaagcagtag tcacttccgc ttaaatcttg
     7021 cgcaaacatt taactcgttt ggcgcaatta tcgcggttgt ctttgggcaa agtcttattt
     7081 tgtctaacgt gccacatcaa tcgcaagacg ttctcgataa aatgtctcca gagcaattga
     7141 gtgcgtataa acacagcctg gtattatcgg tacagacacc ttatatgatc atcgtggcta
     7201 tcgtgttact ggtcgccctg ctgatcatgc tgacgaaatt cccggcattg cagagtgata
     7261 atcacagtga cgccaaacaa ggatcgttct ccgcatcgct ttctcgtctg gcgcgtattc
     7321 gtcactggcg ctgggcggta ttagcgcaat tctgctatgt cggcgcacaa acggcctgct
     7381 ggagctattt gattcgctac gctgtagaag aaattccagg tatgactgca ggctttgccg
     7441 ctaactattt aaccggaacc atggtgtgct tctttattgg tcgtttcacc ggtacctggc
     7501 tcatcagtcg cttcgcacca cacaaagtcc tggccgccta cgcattaatc gctatggcac
     7561 tgtgcctgat ctcagccttc gctggcggtc atgtgggctt aatagccctg actttatgca
     7621 gcgcctttat gtcgattcag tacccaacaa tcttctcgct gggcattaag aatctcggcc
     7681 aggacaccaa atacggttcg tccttcatcg ttatgaccat tattggcggc ggtattgtca
     7741 ctccggtcat gggttttgtc agtgacgcgg cgggcaacat ccccactgct gaactgatcc
     7801 ccgcactctg cttcgcggtc atctttatct ttgcccgttt ccgttctcaa acggcaacta
     7861 actgaacata ttttccgaat aaagtgagga atctgtaatg aaaaaaatca gcttaccgaa
     7921 aattggtatc cgcccggtta ttgacggtcg tcgcatgggt gttcgtgagt cgcttgaaga
     7981 acaaacaatg aatatggcga aagctacggc cgcactgctg accgagaaac tgcgccatgc
     8041 ctgcggagct gccgtcgagt gtgtcatttc cgatacctgt atcgcgggta tggctgaagc
     8101 cgctgcttgc gaagaaaaat tcagcagcca gaatgtaggc ctcaccatta cggtaacgcc
     8161 ttgctggtgc tatggcagtg aaaccatcga catggatcca acccgcccga aagccatttg
     8221 gggctttaac agcactgaac gccccggcgc tgtttacctg gcagcggctc tggcagctca
     8281 cagccagaaa ggcatcccgg cattctccat ttacggtcat gacgttcagg atgccgatga
     8341 cacatcgatt cctgccgatg ttgaagaaaa actgctgcgc tttgcccgcg ccggtttggc
     8401 cgtcgccagc atgaaaggta aaagctatct gtcgctgggc ggcgtttcga tgggtatcgc
     8461 cggttctatt gttgatcaca acttctttga atcctggctg ggaatgaaag tccaggcggt
     8521 ggatatgacc gaactgcgtc gccgtatcga tcagaagatt tacgacgaag ccgaactgga
     8581 aatggcactg gcctgggctg ataaaaactt ccgctatggc gaagatgaaa ataacaaaca
     8641 gtatcaacgc aatgccgagc aaagccgcgc agttctgcgc gaaagtttac tgatggcgat
     8701 gtgtatccgc gacatgatgc aaggcaacag caaactggcc gatattggtc gcgtggaaga
     8761 atcacttggc tacaacgcca tcgctgcggg cttccagggg caacgtcact ggaccgatca
     8821 atatcccaat ggtgacaccg ccgaagcgat cctcaacagt tcatttgact ggaatggcgt
     8881 gcgcgaaccc tttgtcgtgg cgaccgaaaa cgacagtctt aacggcgtgg caatgctaat
     8941 gggtcaccag ctcaccggca ccgctcaggt atttgccgat gtgcgtacct actggtcacc
     9001 agaagcaatt gagcgtgtaa cggggcataa actggacgga ctggcagaac acggcatcat
     9061 ccatttgatc aactcaggtt ctgctgcgct ggacggttcc tgtaaacaac gcgacagcga
     9121 aggtaacccg acgatgaagc cgcactggga aatctctcag caagaggctg acgcttgcct
     9181 cgccgctacc gaatggtgcc cggcgatcca cgaatacttc cgtggcggcg gttactcttc
     9241 ccgcttcctt accgaaggcg gcgtcccgtt caccatgact cgtgtcaaca tcatcaaagg
     9301 cctgggaccg gtactgcaaa tcgcggaagg ctggagcgtg gaattgccga aggatgtgca
     9361 tgacatcctc aacaaacgca ccaactcaac ctagccaacc acctggtttg caccgcgcct
     9421 cactggtaaa gggccgttta cggatgtgta ttcggtaatg gcgaactggg gtgccaacca
     9481 cggagttctg accatcggcc acgttggcgc agactttatc actcttgcct ccatgctgcg
     9541 tatcccggta tgtatgcaca acgttgaaga gaccaaagtg tatcgtcctt ctgcctgggc
     9601 tgcgcacggc atggatattg aaggccagga ttaccgcgct tgccagaact acggtccgtt
     9661 gtacaagcgt taatacatta ctccctgatg tgatgcccgg tcgctccggc taccgggcct
     9721 gaacaagcaa gagtggttag ccggataaac aatgttatcc ggctatattg caggagcgat
     9781 tatgaaacaa gaagttatcc tggtactcga ctgtggcgcg accaatgtca gggccatcgc
     9841 ggttaatcgg cagggaaaaa ttgttgcccg cgcctcaacg cctaatgcca gcgatatcgc
     9901 gatggaaaac aacacctggc accagtggtc tttagacgcc attttgcaac gctttgctga
     9961 ttgctgtcgg caaatcaata gtgaactgac tgaatgccac atccgcggta tcgccgtcac
    10021 cacctttggt gtggatggcg ctctggtaaa taagcaaggc aatctgctct atccgatcat
    10081 tagctggaaa tgtccgcgaa cagcagcggt tatggacaat attgaacggt taatctccgc
    10141 acagcggttg caggctattt ctggcgtcgg agcctttagt ttcaatacgt tatataagtt
    10201 ggtgtggttg aaagaaaatc atccacaact gctggaacgc gcgcacgcct ggctcttcat
    10261 ttcgtcgctg attaaccacc gtttaaccgg cgaattcact actgatatca cgatggccgg
    10321 aaccagccag atgctggata tccagcaacg cgatttcagt ccgcaaattt tacaagccac
    10381 cggtattcca cgccgactct tccctcgtct ggtggaagcg ggtgaacaga ttggtacgct
    10441 acagaacagc gccgcagcaa tgctcggctt acccgttggc ataccggtga tttccgcagg
    10501 tcacgatacc cagttcgccc tttttggcgc tggtgccgaa caaaatgaac ccgtgctctc
    10561 ctccggtaca tgggaaattt taatggttcg cagcgcccaa gttgatactt cgctgttaag
    10621 tcagtacgcc ggttccacca gcgaactaga tagccaggca tggttgtata acccaggtat
    10681 gcaatggctg gcatccggcg tgctggaatg ggtgagaaaa ctgttctgga cggctgaaac
    10741 accctggcaa atgttgattg aagaagctcg tctgatcgca cctggcgcgg atggtgtgaa
    10801 aatgcagtgt gatttattgt cgtgtcagaa cgctggctgg caaggagtga cgcttaatac
    10861 cacgcggggg catttctatc gcgcggcgct ggaagggtta actgcgcaat tacagcgcaa
    10921 tctacagatg ctggaaaaaa tcgggcactt taaagcctat gaattattgt tagtcggtgg
    10981 aggaagtcgc aacacattgt ggaatcagat taaagccaat atgcttgata ttccggtaaa
    11041 agttctcgac gatgccgaaa cgaccgtcgc aggagctgcg ctgttcggtt ggtatggcgt
    11101 aggggaattt aacagcccgg aagaagcccg tgcacagatt cattatcagt accgttattt
    11161 ctacccgcaa actgaacctg aatttataga ggaagtgtga aatgctgaaa acaatttcgc
    11221 cgttaatttc tcccgaacta ttgaaagtgc tggcagagat gggacatgga gatgaaatta
    11281 ttttttccga tgctcacttt cccgcccatt cgatgggacc gcaggtgatc cgcgctgatg
    11341 gcctgttggt gagcgacttg ctccaggcga ttatcccgtt atttgagctg gacagttatg
    11401 ccccgccgct ggtgatgatg gcggcggtag agggtgacac tctcgatcct gaagtagaac
    11461 gacgttaccg taatgcgctt tcactacaag ccccgtgtcc tgacatcatc cgtatcaatc
    11521 gttttgcgtt ttatgaacgg gcgcaaaaag cctttgcgat cgttatcaca ggcgaacgag
    11581 cgaagtacgg gaatattctt ttaaaaaaag gggtaacacc gtaatctcat accggtacgc
    11641 ccgcatgacg cgggcggtta tcgaatgatg gggtgaaaaa tatgaaagcg gcacgccagc
    11701 aagcgatagt cgacctgttg ctgaaccata ccagcctgac cacggaagct ctctctgaac
    11761 agctaaaggt cagtaaagaa accattcgtc gcgatctcaa tgaattacag acgcagggta
    11821 aaattctgcg caatcatgga cgcgctaaat atatccaccg tcaaaatcaa gacagtggcg
    11881 atccctttca catcaggctg aaaagccatt atgcgcataa agcagatatc gcgcgcgagg
    11941 cgctcgcgtg gattgaagaa gggatggtga tagccttaga cgccagttca acttgctggt
    12001 atctggcacg ccagttgcct gacatcaaca ttcaggtctt caccaatagc catccgattt
    12061 gccatgaact cggtaaacgc gaacgcattc aactgatcag ttccggcggc acacttgagc
    12121 gcaaatatgg ctgttacgtc aatccctcgc tgatttccca acttaaatcg ctggaaatcg
    12181 atctgtttat tttttcttgt gaagggatcg atagcagcgg cgcactgtgg gactccaatg
    12241 cgatcaacgc tgattacaaa tcgatgctat taaaacgtgc cgcgcaatcg ttgttattga
    12301 ttgataaaag taaatttaat cgttcagggg aagcccgcat cgggcatctg gatgaggtaa
    12361 cgcacattat ttctgatgag cgccaggttg caacttcttt ggtaacagcc tgacggaaag
    12421 ggtagcaggc cggagacgac gccccggcct tgcctgttat cgctcgtcgc gacgaccacc
    12481 caccgcagcc cagatgcggc ggacgtgcac cgtcacttct tcgcgatcgt gatacaactg
    12541 ccgcgcctga atctgagcat ttatgccatg ttcatcaagc tgtgcctgaa tatacgccag
    12601 attgtgtgat acttcttcgt agcgtttttt catcggcagt ttgagattga aaatggtttc
    12661 acggcaccag ccattaacca gccactgcgc catcaatgcc gcaactttag ccggtttttc
    12721 aaccatatcg cataccatcc aggagatatt gctgcgtgtc ggacggaatt tgaaaccgtc
    12781 ttcccgcagc cacgtcacct gcccggtatc catcagactt tgcgccatcg ggccgttgtc
    12841 gacggaataa acccacatgt tgcgcttcac cagttggtag gtccagccgc cagggcaagc
    12901 gcctaaatcc accgcccaca tcccgttcgc caggcgttca tcccactcat ccgcaggaat
    12961 aaacacatga aatgcctctt ccagtttgag cgtggaacga ctcggcgcct ctgccggaaa
    13021 tttcaggcgc ggaatgccca tatagaacgg cgaattattg ttgctgtatg agtaaccggt
    13081 atagcagcat cctggtgcaa taaagaatac atgcacaacc ggacgcttcg gcgtttcata
    13141 gttcgccagc accccagcat cgcgcaaggc agcgcgtagc ggaacagtga acttacggca
    13201 gaatttcagc aactctttgc tttcgttggt atcggcaact tcaacacgca gttcaccgcc
    13261 cttctctact acaccctgta acatgccgac aatgggggta atacgatctt ctggcggcaa
    13321 atgctgcagg agttccccca ccacaaacca ctggcgggca aaaattaatg aactgaacgg
    13381 cagctcacgg attaacttat cgccatcatc aggttgataa cattcataaa tgacataacc
    13441 cgcattctct ttcacgcggg caaaaccgaa aatttcccgc tggccggctt tatcggtaat
    13501 ttctgcggcg cactcttttt caaagcccgg acggcacagc aatacaacct tattcatgac
    13561 ttacgccctt gcgctttaaa cggatagcac caactaacat taacgcccag cccgcaagga
    13621 agctcacgcc gccaaccggt gtgacaaacg cccacaaacg cagatgggac agcgccaggc
    13681 aataaaggct gccgctgaac agcaccgtgc ctaacgcgag gaaaacgcta ctccagtaaa
    13741 accagatact gatgcgacgc tgcattgcca ccgccagacc taagatcgcc agcgtatgaa
    13801 acgcctggta ttcgagaccg gtttggatcc agcccatctc aacggccccc atggttttac
    13861 ttaacacatg cgcaccaaaa gcgcccagtg ccacaaaaat gaagccgcta atggcggcga
    13921 aaatcagcat aaaacggctg gtcatggtcg taccctacgt aaattattgt tcataacgaa
    13981 agcggaattt ttcttgttca gcagcggctt tcgccaggat ccattggcga aaggcggcta
    14041 ttttacccag ttctgcctgg ctgtcatgac aaaccagata aaaagcattt ttactgacca
    14101 gaacatcatt aaacgggcaa acaagacgtc cggcctcgat ttcagattgc gccatcacgt
    14161 tatttgccag cgccactccc tgcccgtgaa tagccgcttg cagcaccatg gcgctatgac
    14221 taaaaattgg cccttgctga acgttgatat gatttaaccc caactgtcgg gtatatgtct
    14281 gccagtcacg gcgtgacgca tcatgtaata acgtatgttt agccagatct tccggggtct
    14341 tcaagggttt ttcgcctgtc agcagtagcg gcgaacacac cggcaataaa tattcggcgt
    14401 acagtttttc cacccgtagc cccggccagt tgccccgacc ataaaatatc gccacatcaa
    14461 catcatccgc cagcttatct tcctgacgat caaccgcctg gattcgaacg tcaattcccg
    14521 gataagctga attaaagctg gaaagtcgcg gaaccaacca atgaatggcg aaactgggga
    14581 gtaaactgac cgtcaacgcc cccttggcgc tacgggcctg gagtttacgc gtcgcttcgg
    14641 ttaattgcga aaatatctct ttgatatcga ggaaatagct ttgcccttcc tcggtcagca
    14701 ggagtgaacg attacggcgg cggaacagtt ttagccccaa aaaatcctca agagacttga
    14761 tttgatgact tactgcggcc tgggtcacaa aaagctcttc tgctgcgcga gtgaaactta
    14821 aatggcgtgc tgcggcatca aaaactcgta aggcatttag cggtggtaat cgtttagaca
    14881 tggcttttaa actttgatgt taaatgaatt taacaattag atcacactgt gtaacctatt
    14941 agttttttta atctgagcca ttataaattg tccgttgagc ttctaccagc aaatacctat
    15001 agtggcggca cttcctgagc cggaacgaaa agttttatcg gaatgcgtat tctggtgaac
    15061 ttttggctta cggttgtgat gttgtgttgt tgcgtttgca attggtctgc gattcagacc
    15121 atggtagcaa agctaccttt tttcacttcc tgtacattta ccctgtctgt ccatagtgat
    15181 taatgtagca ccgcctaatt gcggtgcttt tttttacctt gcgatcgcga attactgttc
    15241 cagttcgacc atctctttca cgtcagtacg gttgatctgc tgtttgttgc cattagcgtc
    15301 tttatacgaa atcataccgg tatcgttatc agtctgtggt ttgccgtcag agacgatggt
    15361 acgtccgtca ttggtgtgca tcacatagtt cgaaccggaa caggcgctca gggcaaaagt
    15421 cagcatacag gcagaaataa ttgcggcagt ctttttcatc atcttctcct taaagcgatt
    15481 aataaatctt aatagtattg attaacaggc taaaattaac gcctaacact attcagcata
    15541 tgttacttac gcggcttcgc caggatatcc agataattct gatggttagc actctccttg
    15601 tatcaaagtg aattttgcgt cacgatcggt gcatcaagcc gaggagtacc atgaacgttt
    15661 ttaatcccgc gcagtttcgc gcccagtttc ccgcactaca ggatgcgggc gtctatctcg
    15721 atagcgccgc gaccgcgctt aaacctgaag ccgtggttga agccactcga cagttttaca
    15781 gcctgagcgc cggaaacgtc catcgcagcc agtttgccga agcccaacgc ctgaccgcgc
    15841 gttatgaagc tgcacgagag aaagtggcgc aattactgaa tgcaccggat gataaaacta
    15901 tcgtctggac gcgcggcacc actgaatcca tcaacatggt ggcacaatgc tatgcgcgtc
    15961 cgcgtctgca accgggcgat gagattattg tcagcgtggc agaacaccac gctaacctcg
    16021 tcccctggct gatggtcgcc caacaaacta gagccaaagt ggtgaaattg ccgcttaatg
    16081 cgcagcgact gccggatgtc gatttgttgc cagaactgat tactccccgt agtcggattc
    16141 tggcgttggg tcagatgtcg aacgtgactg gcggttgccc ggatctggcg cgagcgatta
    16201 cctttgctca ttcagccggg atggtggtga tggttgatgg tgctcagggg gcggtgcatt
    16261 tccccgcgga tgttcagcaa ctggatatcg atttctatgc tttctctggt cacaaactgt
    16321 atggcccgac gggtatcggc gtgctgtatg gcaaatcaga actgctggaa gcgatgtcgc
    16381 cctggctggg tggcggcaaa atgattcacg aagtgagttt tgacggtttc accactcaat
    16441 ctgcgccgtg gaaactggaa gcaggaacgc caaatgtcgc tggcgtcata ggattaagcg
    16501 cggcgctgga atggctggca gattacgata tcaaccaggc cgaaagctgg agccgtagct
    16561 tagcaacgct tgcagaagaa gcgctggcga aacgtccagg ctttcgttca ttccgctgcc
    16621 aggattccag cctgctggcc tttgattttg ctggcgttca tcacagcgat atggtgacgc
    16681 tactggcgga gtacggtatt gccttgcggg ccgggcaaca ttgcgctcag ccgctactgg
    16741 cagaattagg cgtaaccggc acactgcgcg cctcatttgc gccatataat acaaagagtg
    16801 atgtggatgc gctggtgaat gccgttgacc gcgcgctgga attattggtg gattaatgac
    16861 aaacccgcaa ttcgccggac atccgttcgg cacaaccgta accgcagaaa cgttacgcaa
    16921 taccttcgca ccgttgtcgc aatgggaaga taaatatcgc cagttgatca tgctggggaa
    16981 acagcttccg gctttgccag acgagttaaa agcgcaggct aaagagattg ccggatgcga
    17041 aaaccgcgtc tggctgggat ataccgtggc tgaaaacggc aaaatgcact tctttggcga
    17101 cagcgaaggg cgcattgtgc gcggcctgtt ggcggtgttg ttgactgccg ttgaggggaa
    17161 aaccgccgcc gaattacagg cacagtcgcc gctggcattg tttgatgagc tgggattacg
    17221 tgcgcagctt agcgcctcac gcagccaggg gttaaatgcg ttaagcgagg cgattatcgc
    17281 tgcgacgaag caggtttaag cacagaaccg ggtcggataa gacgttcgcg tctcatccga
    17341 cctgattgtt taaccctgac gcgccgcttt cgccatcatc ttcttcagcg catgagaaac
    17401 cgcaacaaac ccaaaggtgg cggtcaccat cgttgccgcg ccaaatccag atgcacaatc
    17461 catccgcttc ggcccttctg ccgtggcttt catcgcacac accgtaccgt ctgactgcgg
    17521 gtacaccagc gcttcagtag aaaacacgca atccacgccg agcttacctt tactgttttt
    17581 cactacgcca aaatcgcttt tcaggcgctc gcgcaacttc gccgccagcg gatcctgaat
    17641 cgttttcgcc agatcggtaa cctgaatctg cgtcgggtca atctgcccac ccgcgccacc
    17701 ggtcgtaacc agcgggattt tattgcgccg acaataagca atcagcgccg ctttgggccg
    17761 tacactatca atggcatcaa tcacgtacga ataaccggca ctcatatatt gcgctacgtt
    17821 atccggcgtc acgaaatcat ccaccaccgt tacacggcac tccgggttaa tctggcgaat
    17881 gcgctccgcc atgacttccg cttttgccag cccgacgtta tcgcgcagag cgtgaatttg
    17941 ccgattggta ttggtgacgc acacatcatc catatcgata agcgtgattg cgccaatccc
    18001 cgtgcgcgcc agcgcttccg ccgcccagga accgacacca ccgataccaa ccacacaaat
    18061 atgcgcgtca gcaaacagtt gcaacgcttt ttcaccatac agacgcgctg tgccaccaaa
    18121 acgctgacgc catgcatcac taattaccac agacatagca cctcagaatt aaaaagggcg
    18181 aggttatccc tcaccctaaa cgaataaatg cctgatgcgc tacgcttatc aggcctacat
    18241 aactcctgca atattttgga tttgcatgat ttgtaggccg gataaggcgt tcacgccgca
    18301 tccggtatga acaacgcgca cttagtcaat aatcaggatg tgaggttacc cctcaccctg
    18361 tcatatccgt aaaaacggca tacagaatat cacatcagcc gctaaagacg ttacctgcgc
    18421 ccggagccgt tttcagcacc caaacccgtc catagtggtt gtaccaacct gcgcggtgtc
    18481 cggcttccgg cccgatccct tgatagatat cgaagtgttg gcctttgatt gcgcccccga
    18541 catccaacgc caccatcaga cgcaattcgt actgaccgtt aaatttgccg ttattatcca
    18601 gcagcggcac ttctgccagc aaggtagtac ctggcggaat aattgaacga tcggaggcaa
    18661 cagaagcacg gccaaccagc ggcaccgcac ttgccccttt caccggtgca aaagattgcg
    18721 gtttaaagaa gacgaaagac gggttctgtt ccagcagctc gcgaacctcg gcttcactgt
    18781 gtgtttcgcc ccaatgacga atcgcctgca tcgacatatc ttcttttttc acttcgccac
    18841 ggtcgatcag caccttacca atgctgcgat aggcatgacc gtttttccct gcatagctga
    18901 aaaagttaag cggactgcca tcaccaaagt cgatataccc actaccctgc acatccataa
    18961 tgaagttatc catcagagag ttgctgtaag cgaggatgta tttatcgccc aatgcccccg
    19021 cgtagatctc cgcacgagac ggcagacgac cacgttttgg cggcatacgg taaataggat
    19081 actggaactc gccctggcgg gtatggcgcg cctgaattac cggcgtgtaa tagcctgtaa
    19141 actgcacgtt accatagttg tcggcacctt ccatctgcca ggcatcaatg ccgaactggc
    19201 gcatattgcg ggtatcaccg cctgcgcgca gccactcttg caccgcgtta taaacattac
    19261 tctggttgcc atacagacgc ggtgacgaat tacggatatg gttaatttgc tcggcaaaat
    19321 caccggcgtt aatcggcgcg ccaacggcat ctggctggtt caccagagag aaaggctggg
    19381 taaatttccc gtctttatat tgctgtccgc gatcggttgg tttggaagag caggcggcaa
    19441 gcattgccac aaccgtgccc ataagaaggt actttaccca acgtcctttc attgttctct
    19501 tcttcaggtt aaaaaataag gcgcaacgaa gataacaaac cgccgcgttt aatgaaatga
    19561 gcagcctctc cctgacgcaa attttgcaca aaaaataggc tttagtggtt tgtttttgtt
    19621 caaaatcatg ctaaatccgt gatcggggta aaaaaaaggt tgcatgaaaa cgcgagcgga
    19681 gtatagtgcg catccacgga cgcggggtgg agcagcctgg tagctcgtcg ggctcataac
    19741 ccgaaggtcg tcggttcaaa tccggccccc gcaaccaatc aaatttgatg aagtaaaagc
    19801 agtacggtga cgcggggtgg agcagcctgg tagctcgtcg ggctcataac ccgaaggtcg
    19861 tcggttcaaa tccggccccc gcaaccaatt attgaacacc ctaacgggtg tttttttgtt
    19921 tctgatctcc cataaaaaag cgccattcag cgccttttta tcatcccctt ctcgccagcg
    19981 ttgccccatc ggcaaaatac gctttaatcc ccgcaagaat agactccgca acttcctgct
    20041 ggaaagtcgc cgttttcagt ttacgctctt cctcaacgtt actgataaac gccgtttcga
    20101 ccagaatgga gggaatatct ggtgccttta gtacggcaaa cccggcctgt tcaacttgat
    20161 ttttatgcag cttgttgatt tttccgagct tattcagtac cgctttacca aacttaaggc
    20221 tgtcagcaat agtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac
    20281 cgcttttgct cacgccacca atcaagtccg aggcgttctg ggtttgtgcc agatattttg
    20341 ccgcagtact ggttgcgcct ttggttgaga gcgcaaacac cgaggaaccg ctcggctgac
    20401 gactggtaaa ggcgtcggca tggatagaga caaacaggtc agcacgctgt ttctgggctt
    20461 ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca
    20521 tattgccctc tttctcgatc agagagcgca aacggcgagc tatttgcaat accacatctt
    20581 tttcgcgcgt tttgtatttc cccaccgcac cggagtcttc gccaccgtgg ccagggtcaa
    20641 gcataatgac aatcggacga tcgcgccctg ctttacccgg ttgtggacca ctttgcgctg
    20701 gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt
    20761 cctgcatatc ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg
    20821 cgaccggcgc aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatgc
    20881 gtacggtttg cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtctgcac
    20941 gaatttgtgc cgccatcccc ttgagtaccg agttcaggtt tacatcttcg atatccacca
    21001 ccacgcgttc agggttactc aacgcgaact gcttatattt cagctgacga tttgattcta
    21061 ccgtcacgcg ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctaaccg
    21121 cagccaggct gacctgactt acgctcaata gccacatagc gcccgcgcct tgcagtaaat
    21181 gacgacggct gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa
    21241 gctgtgtaac aagtaaacga ctaatttgac cggaaacttt agcgaaagac gcataatctg
    21301 tcatctaata aacggtaaac attcttttta tattcacggc attactgata aaaaagtcgc
    21361 tctcgcataa aattcacact tgcaccctgc gaaaaaacag aataaaaata caataatttc
    21421 gaataatcat gcaaaggggt gtgccgtggt aaaggaacgt aaaaccgagc tggtcgaggg
    21481 attccgccat tcggttccct atatcaatac ccaccgggga aaaacgtttg tcatcatgct
    21541 cggcggtgaa gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttgtt
    21601 gcacagcctc ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa
    21661 tctggctgcg catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa
    21721 aacactggaa ctggtgaagc aggctgcggg aacattgcaa ctggatatta ctgctcgcct
    21781 gtcgatgagt ctcaataaca cgccgctgca gggcgcgcat atcaacgtcg ttagtggcaa
    21841 ttttattatt gcccagccgc tgggcgtcga tgacggcgtg gattactgcc atagcgggcg
    21901 tatccggcgg attgatgaag acgcgatcca tcgtcaactg gaaagcggtg caatagtgct
    21961 aatggggccg gtcgctgttt cagtcactgg cgagagcttt aatctgacct cggaagagat
    22021 tgccactcaa ctagccatca aactgaaagc tgaaaatatg attggttttt gctcttccca
    22081 gggcgttact aatgacgacg gtgatattgt ctccgaactt ttccctaacg aagcgcaagc
    22141 acgggtagaa gcccaggaag agaaaggcga ttacaactcc ggtacggtgc gctttttgcg
    22201 tggcgcagtg aaagcctgcc gcagcggcgt gcgccgctgt catttaatca gttatcagga
    22261 agatggcgcg ctgttgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat
    22321 ggaaagcgcc gagcagattc gtcgcgcaac aatcaacgat attggcggta ttctggagtt
    22381 gattcgcccg ctggagcagc aaggtattct ggtgcgccgt tctcgcgagc agctggagat
    22441 ggaaatcgac aaattcacca ttattcagcg cgataacacg actattgcct gcgccgcgct
    22501 ctatccgttc ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta
    22561 ccgcagttca tcacggggcg aggttctgct ggaacgcatt gccgctcagg cgaagcagag
    22621 cggcttaagc aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg
    22681 atttaccccg gtggatattg atttactgcc cgagagcaaa aagcagttgt acaactacca
    22741 gcgtaaatcc aaagtgttga tggcggattt agggtaagcg atgaaaatca ccggatgcgg
    22801 caggcgtaac actcgtgcgt cgcatccggc aattacgttt attcccgcga actaaacaac
    22861 gccgccagac cactgcgccg ctcagtacga gtggcgattg ccgcacttaa tatacgctca
    22921 tcggcataca gcgacagacg acgacgcgcg cgggtcaccg cggtgtatac cagctctcgc
    22981 gttactaccg gcgtgcgttg gctcggtaaa atcaacgccg catggtcgaa ctccgatccc
    23041 tgcgatttat gtaccgtcat cgcccacgta gtttcgtgct ctggcaagcg actcggttgc
    23101 acagacttaa tattgccgtc cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc
    23161 agcgcaatac cgatatcgcc attaaacaac ccaagcgcgc tgtcattacg ggcaatcatc
    23221 accggtcggc cttcgtacca acgagagtgc ggatgacgat gaattttgcg cttctgttgc
    23281 ataaactgct caattcgctc attcagtcca gccacgccaa acggcccttc ccgcagggcg
    23341 cacaaaagct ggtactcatt gaacgcctga atgattaaat ccggctcggc acgcgcttgc
    23401 agcagatcca gataacgtcc gtaacccgca agagcttcct caaacatcgc aatataatct
    23461 tcgccgctct gtaaaagtcg tttttcgata tcagtaaaat cctgctgaaa aacagttttc
    23521 actgccgttt tatcaccacg gttgatcgcc gcagctaact gaccaatgcc agaatcgctg
    23581 ccgaaacgat agcttttttg cagcaggcag agactgtcgc gcaaagatgc cgcttctgtg
    23641 ccggttcctg ccggaacgtg agtccccgtc aggcggctta gctgcctggc acgctcggcg
    23701 gtaaagcccg cgttggcata agcgcagata tcgcccagca cagccccagc ctcaaccgag
    23761 gccagttgat cacgatcgcc gagaaagatc actcgcgcat gatctggcaa ggcgtcgatc
    23821 agtctcgaca tcataggcag atcgatcatt gacgcttcat ctaccaccag cacatcaaga
    23881 tgcagcgggt taccggcatg atgacgtaaa cgctggctac ccggctgcgc gcccagcaat
    23941 cggtgcaaag tgctggcatc ttccggaatg cgtttctttt gttcatcggt cagcggtaac
    24001 tgtcgcaaag ccttgccgag agattcggtt aagcgcgcgg cagctttacc cgttggtgca
    24061 gccagacgga tacggcagcg ttcgccgtcg gccatttgaa ttaacgctgc cagcaacttc
    24121 gccacggtgg tcgttttacc ggtgcctggg ccgccggaaa tcaccgagat ccgccgcgtc
    24181 agcgccactg ccgctgcaac tttttgccag ttaatttcat cgcttactgg aaaaagtttg
    24241 tccagggttt gcgccagtag agcttcatca acctcaatgg catgattcac ctcattgaag
    24301 aagcgcgcta ccgtccgctc gttacaccac atgcgattca aataaagacg atcgccacag
    24361 aggatcatcg gcgtgggttc atctcccctg ctgaccgctt gagaagccag caagcattct
    24421 tcccaatttt gtagctcacc gatttcactg acacaggtcg ccaacagcgg atgcgacgcc
    24481 tcgttatttt ccagtcgtga aagcggcaaa cagacgtgtc cctctccggc atcatgactt
    24541 aacagtgccg ccgcgagggt gacggcagga tgttcatctc ccgccacggt cagggcaaat
    24601 tgcacatcca gcgggcgtag ctgtttgtgc tccacagctt ccagtaattg cttttgcaat
    24661 ttcattactc ctcctccagg gtcataccgg caaacatctc atccatcagg gcaatcaacc
    24721 cggcgttggg tcgggttgtg taaatccctt gttgcggatg ttctttatca acgccacgca
    24781 ggaacagata gataacgccg ccaaagtgaa gatcatagtc gtaatcagca atgcgatggc
    24841 gcagataacg atgcagcgcc agggtataaa gctgatattg cagatcatag cggtgtgcct
    24901 gcatcgctgc tgccatagcc tgttgggtgt aagccgaact gtcttcaccc agccagttgg
    24961 atttatagtc gagcaggtaa taacgccctt cgtggcggaa taccaggtcg ataaagcctt
    25021 ttaacatgcc gcgtacctgc atgaactcca gcggcgggca gccagcggat agcgggtcaa
    25081 actggcgaat tagtgcatca agctggctgg cgatgagcgg ttcgctaatc ggcaggtaaa
    25141 actccatctc cacctgttta tcgcggtcgg aaagctgact caggctaaca cccgtttcat
    25201 tgagaggtgc ctggaggaca gccgtgatcc actcggtcaa caccggttcc cactgcgatt
    25261 caaagccgcc gagttccagt ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat
    25321 cgaggtcttc aaacaaactg tgcaagaatg tccccggtga cgcaccgcgc gggaactgat
    25381 gcggtgttaa cgtcggttct tcaacgacgc tgaccacgcc tgcggcatca acatccagcc
    25441 gtggcatcag atcctgagcg ataccgtgac cacgctgctg caagccggag tagctggtga
    25501 cacgccagtt atcgccgggc aatcgttgta gtgtcctcgc attcagttct gcagttaaag
    25561 catcattaac ctgccagggc tggttatcac cagtttgtgc cgtttgccag gcaatatcat
    25621 catcgcataa cgcttcaata caggtgcgaa gccctgccgc atcttgcggt tccccttttt
    25681 gcagcaaacg cccgagcgca ctttggtgga cgtcggtgtc acctttttta tcgccacgac
    25741 ggcgcaccag cggtgcaacg ccgagactgc aatgccaaac cgaacgcgtc agcgccacgt
    25801 aaagcaaacg cagatcttcc gccagacgtt cgacctccgc gaggtcgacg ctttctggcg
    25861 cagcattaag atccaaaact gcctcaaacg agtggcgatc gtgataaaac gcctgatcct
    25921 ggacgcggaa attggtaata aatggcaacc agaccagcgg atattccagc cctttcgatt
    25981 tatggatcgt gacaatctgc accagatgtt tatcactttc gaggcgcatt tgttggctgg
    26041 aggcattact gtctggctcg aggatatgtt gcgataacca gcgtaccagc gcatgttcac
    26101 tttccagctg cgttccggct tcttgaagca gttcgctgat atgcaagata tcggtaagac
    26161 gccgctcacc gcctgccgtt gccagcaagt tttctgcaat gttacgcgcc gacatcagcg
    26221 cccgcagcat cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt
    26281 cgactaccgc atcccacgca tgttcgtcat tattcagcgt ttcaatatcc agcgcattca
    26341 gccccatcat tgacgttgcc agcgcactgc gcagggtgtt ctcacgttcg ggcgtcatca
    26401 ccgcctgcaa caaccaaagc atttcctgcg cttccagagt ttcaaaaaca ctgtcgcggt
    26461 tcgaaaggta aacggaaggg atttccagca acgttaaggc atcgcgcacc tgggcggcct
    26521 cctggcggct gcgcaccagc acgctgatgt ccgaagcacg caccggacgc gcgtcgtcgc
    26581 cgttcatcag caacgcttcg ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag
    26641 cacatacctg cgccatggta ctttgatagt cgccaacgcc gcagctttcg ccttccatca
    26701 gccacatttt catcgcaggc tgcgtttcac ctttaaatac aaaacgtaac gcctgatttt
    26761 tcccggctga tttcactgga ataaacggta tttcgcgaaa catgaacgcg tcatcagtct
    26821 ggctgaaaag cttgttcaca ctgttcacca ttccaggtgc ggagcgccag ttggtgtcta
    26881 aagtgtagtg ggcgtgaact tcactacgcg ccttcatata agtgaagata tccgcacccc
    26941 ggaatgcata tatggcctgc ttcgggtcgc caatcagcaa caatgcggtt tccggctgat
    27001 ggtgccagat acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcga
    27061 tcattgccac cgggaatcgc gtacggatcg ccgctgccaa cacctcgccg ctttcgctac
    27121 gcagcgcgga atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt
    27181 tttcacgcgc tactgtttcg cggatctcag ccaatgcgcg ggtgatcacc agatcgcgga
    27241 tcgacaatgg ttctgcaagc agttgatcga tcgcctcaaa cagtggatgt cgcggggttt
    27301 cccccccggc cttcgtgcga tcttctaaga aacgctggga gaatttttcc agcgactccg
    27361 gcaactgata actgtttgtc tcttcttctg cccaggcgct gatcttttcg atccatttag
    27421 cctgattgct acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca
    27481 gttcacccac tgcgtcgcgc cactgctgtt ttaccgtatc aatacgcgcc acaatttgcg
    27541 cgtggcggga agccagcgtt tcatcatcgg gcggcggtgc tttgataacc ggcgcttcgc
    27601 cttgcagata acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga
    27661 cgacctgggc tatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg
    27721 cctggtagcg tagcagagac tcatcttcaa tcagctgctg ctcaaacagc atgccggatt
    27781 caaaggcatt caggttgagc atgcgctggc aaaagccgtg aatagtaaag actgccgctt
    27841 catccatctg ccgttcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct
    27901 cttccagcag gcgttcgtac agtggattgt cggtggtttc acgcagacag gcgatgcgca
    27961 actcgtggat attgctacgg atacgaccgc gcaattctgc cgtggcagcc tcggtaaagg
    28021 tgaccaccag cagttcttca acgatcagcg ggcggggaaa ggcggcggaa ccgcctagtc
    28081 caagtaacag gcgcaaatag agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg
    28141 cttcaatcag gcgctcgccc tgcaagggca agcgcaaagg atctagtgtc tcggcgacat
    28201 cactcattct tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac
    28261 actttccagc cttcaggatg cacatattcg gctttcccgt tctggctgcc ggaaatctgc
    28321 gacagaatag ccatgccttg cggctcgacc accgcctgat ggaagaaatc agcaagtttt
    28381 tgcggcgtca gcagttttat ctgggccacg attttatcac gcgaatcgaa gcgcatattg
    28441 ccgcgatcga aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc
    28501 agcatctggg taattatcgc ctgctggatt tgcgcaaact catctggctt catcgctcgc
    28561 aattttgcct ctgcggttgg gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt
    28621 ttatcattgc tttgcaaaag gaagcccatg ccccactgac gccccacgct cattggaaac
    28681 gcaaacacgg catagcccaa ttgttcttcg gtacgcaact gattgtagaa ccacggctgt
    28741 acgatctgcc ccaacagaga gctataggct gagctggtgt attcatcgta gccagtcggt
    28801 acaaataccg ctgccagtgc ggagtcggtg ctgttaccgt ctttttcaaa gatgacggat
    28861 tgttttttat cgaccactac atctttgttt cgacaccact ctgaaccatc agcgcccaac
    28921 tgtttttgca catcgcgtgc cagcgttgtt gcctgggcct cggtcatgtt gccgataacc
    28981 ataaactctg gtcgagcccc tgattttaag gcgtcgcgat aggccagcac ctctttcaac
    29041 gtaatggagg gcaaaatttt acgccgttca tctcgcgaga agtacggcac ttgcgagagc
    29101 atctgcgcgg gcataatcgc ctgctcaaac gctttaccct tttctgcgga atccatcatc
    29161 tggttatacc aggacttcgc ctgctcaagc tgctcttccg tagcggtata gctaaagtac
    29221 ccctcgagca atgcctggaa cagctgcggc agacgctggg tgtaaccatt agcattaacc
    29281 ataaggccgt tgttagcgtt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt
    29341 aactgatcaa gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg
    29401 gcgctgtcca tggctttcgg attacgcaaa atcaggctga catcagcttt gggctcgctg
    29461 gcaaaataac ggcttggcgc atacaccacg cgcagattcg actcatcaac aatcagctct
    29521 ggatggtcgt atttcttctc tgacttaatc agcgagaaat catcaggaat ataagggtta
    29581 agctctggca aagagagcgc aatgtcggcg gcttttttct gccagtcggc gaaagtttgt
    29641 gcgctgattt tatcgacctg atacggcgca tcgacaaagt aagccgtttt gttgtgcggc
    29701 tctttcgggc tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttcc
    29761 tttactgctt tagcatcgta ccgatcggca atattgactg catccagcgt atgctcaaca
    29821 ggaacgcgaa tcatggtatc tgccagccat tcgacgtaat ccatatcacg ggtgatcgac
    29881 ggataacgga agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcaatg
    29941 cctttttcac gtaacagatt gagatagcta aaaattgccg ccacaacctg atcgcgatta
    30001 gccaggcctt tatcggttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg
    30061 ataggatcgg agttggcgct aatgccctca actaatccct gcttttgcag ccagtcagaa
    30121 agtgtacctg ggctgcgatt gccaatcaga taggtaatca attcatcggt tttactacgg
    30181 aacttcgctg agttgttatc gatgcgaaac tcaacgcgca acactttacg cggcagcgca
    30241 gggacgtaat gaatgataat gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc
    30301 ggttttttgc tctctttgtt cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac
    30361 tccggcagcg gtttattact gtaaataacc gccttcatca aattggcgga atagtacttc
    30421 tcgtggaaat ctttcagcgc ctgctgcacc ggattaccag gtttgtcgct taaagtttcg
    30481 aggttaccac cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcgctgacc
    30541 tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta attcagcgtt cactgcatta
    30601 cgctcacgtt cggcgtattt cttgtcgagc aagggttctg caatagcatc ggccaggcgg
    30661 tctaccgcac cgggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggc
    30721 gcagtgctgg cattgtgact accgccgtgc attttgagat attcggccag actgtcagcc
    30781 tgcgggtact ttttcgaccc catcagactc atatgttcaa ggtaatgtgc cagcccctgg
    30841 tacgcctcgg gatcttccag cgacccaacg ggcactacca gcgccgagag cgatttaact
    30901 gcctgcggat cagaaaccag caagaccacc ataccgttat ccagacgtat agcctgatac
    30961 tggcggttat ctttatcact tttacggatg gtttcctgaa tcggctgcca tcccgtttct
    31021 gcctgactta atggtgccca gagggcaaca aacaacaata atgctttgaa ccaggtgctg
    31081 cggggcattc acggacctca taatcaactt tattttctgg acagattcaa caggttaacg
    31141 caaatcatct gccagaattt aatcttgtgc tgcacgagtc agcctatgtt tatataacca
    31201 tcagtccgtg actggtgcgc atcataaagt aagcggatag attgcgcaat ctttatacaa
    31261 cactcatgac tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat
    31321 ggcctccatt gtctctggtg ttaattgccg ccagagccgt tgataccaga tatcatcacc
    31381 ttcgccacgc accatcatgt tgccttcgta agcctgaagg aatttcgtac gggctttttg
    31441 caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca
    31501 cgcgccgcca ctttcaggta acaccagcaa tggcgcggac attccttcac gatacccctc
    31561 aatcagttgt gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc
    31621 accgtctttg cgtagaaaaa ggcgactttc gccattacca ccgctggcac agtagacaag
    31681 gtgttccagc caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa
    31741 caggccatcc ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggcgag
    31801 atcaatttcc atactctgcc ccggctggcg acaggcaatg actctgtcgg caagctgctg
    31861 catctcctgg cactgtgttt cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc
    31921 tgccgcccgg aagcggcgga acaagcgttc ggcatcatcc tgctcaacca gtgcattcaa
    31981 taactgctga ttgatttgat aacggctaag tccttccaga ataaatggct cggtgtctgg
    32041 gatttcgctg tcttcagtac ggaagttcac ctgcaaacgc atctggaaga acgcccgcac
    32101 cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaacgg
    32161 cagcggctga acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc
    32221 acgagcatag ctttgtcgtt cgcctggctg gtagttttgc ggatcaaacg gcatccgggt
    32281 atggaggcaa gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc
    32341 atcgcccggt agataatgac tttgcccgat gtagtcgatc agttcctgca ccagtaccga
    32401 cgggaaacgt tcactgttat cctgaatgga acgcccgata tagctgatat agagtttttg
    32461 ctgcgcggaa attaacgctt ccaggaacag gtagcggtcg tcatcgcgac ggctacggtc
    32521 gccacgcttc ggtttctggc tcatcaggtc aaagcccaat ggcgcaagct gacgtggata
    32581 aacgccgtcg ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat
    32641 cagagtacaa atgttaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag
    32701 acgctgtgcc agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcacc
    32761 taaaccttcg gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt
    32821 ttccgcatcc ggcaggaaga aggcgttgag catatcgcga caaaccggca accactcttc
    32881 tagcggacgc tcctgtgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaagc
    32941 cagatgcccc accagttctg caattaagcc gctcgattca tcataaggta gaaccgattg
    33001 ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac atacgcgtca ggccaaatcg
    33061 ccaggtgtgt tgtccggtgg cggggagttc cagctcgcga acgttgtcgt catctatccc
    33121 ccaacgaatg ccggattcgt tgacccactg gcgtaaataa cgcagccctt cttcggtgat
    33181 gtcaaaccgc gccgccagca ccggcacatc cagcagcgcc agcacatcct ctgacacaaa
    33241 gcgactgtca ggcagtgata acaggctgat aaacgcttcc agtaccggat gtgactgccg
    33301 cgcacgacgg tcggaaatgg cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac
    33361 tgcctgaata aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg
    33421 agtaagtgtc ggggcttcct ccagcatcgc cagcaagcga tcgtgtaaaa cttcaacttc
    33481 acgctgcggg ctatggcaaa cgtggaatgt gatactgcta tccagtggat caagcgggcg
    33541 tttgttatcg ctacgggaaa actcttcgat gttcacaccc gcaacggcgc ggttttccag
    33601 ttccagaatg tcagactgaa tgttatgcag caggttatct ggcgtcacat cgacaaaagc
    33661 gtccagctcc tggctgctct ccaggtcaga aaggagataa atgtagtcgc gcccaagctt
    33721 cccccatgag gccagcagcg ggttgccgac atcctgttca ccatcgctgt taaagagctg
    33781 tccggcattt tcgctatcgc gaaatagcgg caattcgcga tcttcaaaac tgtgtcgacg
    33841 ctgacgagtc agcagtttcg ccagataagc aggatcttta atatcgcccc agtaataacg
    33901 gcaggggttg gtaaacagga gatggatttc aatatgttta cccagcgcct ggagcgcctg
    33961 gagataaaca ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtaaccccgg
    34021 cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc tgatagagat tcgcgcggtg
    34081 ccagcgcggt tgtccgagtt catgggtata ttccaccagc gtcttccaca acggggcttg
    34141 ccaggcctgt gcttctccca gcccttcaac caaatgtcct gtttcccact gtgccagcca
    34201 gtccggacga tagaccagat actggtcaaa caggtccgcc gcttttgagg aaagctggaa
    34261 cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg
    34321 ctccagcaat tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaggc
    34381 gctctctttg gggatttccg gtaacacccg gacgaacata tcccagataa agctcgctgg
    34441 cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc gacagggtca tttgcagcca
    34501 ctgcgccata ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg
    34561 ttcgcgttcg acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta
    34621 gacccttaac atagcggctc ctgactactg acgattcggg caatgcaggc gggtcatctc
    34681 gccttctctg cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga
    34741 tgtctgcatt cggttgacct gccagttggc aggtggcgaa atcgcgcgca gttgcgtttg
    34801 ctgccaacca tgccgccaga gctgttggta ctggtttctg ctggcgagac tgttcattaa
    34861 tgttcgctgg atacccgata acgcagtgac aatcatcacc atcagcacca tcgcaaccat
    34921 tacctccggc aggctaaagc cttgttgatt cttcagggaa gctgacataa tgccccctct
    34981 ttcagcggac aaaaatcgct ccagccgcgt ggcgaaaaaa caatttttcc atcaatgact
    35041 tcgcctgttc gccacaacga aacgccttca taaccggcaa tcaataaggc ttcattatct
    35101 gccagtaaac gcaaacaaac ctgggcatcg gttccagcgt actgcgagca ctgaactgct
    35161 ggctgcgtct gccagcagtg catttttccc cacgccagcg ccgactgaac gatggcctgg
    35221 cggcgcaatg actgactttc catgctcacg cgagaggcaa aactgcgatc ctgctgactc
    35281 attccttgca atagcaagct acccaaaacc agcagcatca ggaccagagc cagtgacgaa
    35341 acaccttttt cgcggttcac aggttgaatc ctgtcacgct atagctggca tccaccacgg
    35401 tttgcggttc agacttactg gcagcacgca tattaaccgt caacaccggc gagaagccgc
    35461 tgacatcctg acgtacgacc tgaaaagtgt cgataatgat ggcatccgga ttagtgactt
    35521 tatcccagcc cttaccttca caggatgtcg caccgcgtag cgtttccagc acatgctcct
    35581 tcagacgaaa tccaatctgg tcggactctt ttaccggttc gcgatcccag ataccgttac
    35641 tgttcgcatc ccactgcaca atgatacagt caccctgtcc gacaatttcc agcccttcgc
    35701 cggtacagct gccatgacaa taacccgccc tctggagatg cttcgcgacg gtaaataccc
    35761 gcagccagat ttcatcttcc agtgccagct tacgagtgct cgttaaactt tcacgctgta
    35821 acgcaggcag aaagcgtgca gcccccagca acaatacgct actgatcgcc atagcaatca
    35881 acacttccag cagagaaaaa ccttgctctt ttacaggcat ccttctgttt ctccttgctg
    35941 ataaagccgg agtcttcccc acggcgaaac caccagccac cactcgcccg ttgagttttt
    36001 gaagcgaata tgcccggccc atgcggtatt gcgcaggcca aagaaagcaa gcgaaggtgt
    36061 caggtcgctc atttcgactt cgggccagcg tggcacaaag accaatggtg aactgccatg
    36121 acaggtattg gccccagcag cggaactcac aaggcaccat aacgtcccct ccctgataac
    36181 gctgatactg tggtcgcggt tatgccagtt ggcatcttca cgtaaataga gcaaatagtc
    36241 ccgcgcctgg ctggcggttt gccaaagccg ttgcgactgc tgccagtatt gccagccata
    36301 gagtccactt gcgcttagca tgaccaaaat cagcatcgcg accagcgttt caatcagcgt
    36361 ataaccacgt tgtgttttca tgccggcagt atggagcgag gagaaaaaaa gacgagggcc
    36421 agtttctatt tcttcggcgc atcttccgga ctatttacgc cgttgcagga cgttgcaaaa
    36481 tttcgggaag gcgtctcgaa gaatttaacg gagggtaaaa aaaccgacgc acactggcgt
    36541 cggctctggc aggatgtttc gtaattagat agccaccggc gctttaatgc ccggatgtgg
    36601 atcgtagcct tcaatctcaa agtcttcgaa acggtagtcg aagatggatt cgggtttacg
    36661 tttgataatc aacttcggca gcggacgcgg ttcgcggctt aattgcagat gagtttgatc
    36721 catatggttg ctgtacaggt gcgtatcgcc accggtccag acaaaatcac ccacttccag
    36781 atcgcactgc tgcgccatca tatgcaccag taacgcgtag ctggcaatgt tgaacggcag
    36841 gccgaggaag acgtcacagg agcgctgata aagctggcaa gagagtttgc cgtctgccac
    36901 atagaactgg aagaatgcat ggcacggtgc cagcgccatt ttatccagtt cgcctacgtt
    36961 ccacgctgaa acaataatgc ggcgcgaatc cgggtcgttt ttcagctggt tcagtaccgt
    37021 agtaatctgg tcaatatgac gaccatctgg cgttggccag gcgcgccact gtttaccata
    37081 cactggcccg aggtcgccat tttcatcggc ccattcgtcc cagatggtga cattgttttc
    37141 gtgtagataa gcaatgttag tatcgccctg caggaaccac agcagttcgt ggatgatgga
    37201 acgcaggtgg caacgtttgg ttgtcaccag cgggaatcca tcctgcaggt taaaacgcat
    37261 ctgatgacca aaaatggaaa gcgttccggt tccggtacgg tcgtttttct gtgtgccttc
    37321 gtcgagcact ttttgcatca gttctaaata ctgtttcatg gttcctcagg aaacgtgttg
    37381 ctgtgggctg cgacgatatg cccagaccat catgatcaca cccgcgacaa tcatcgggat
    37441 ggaaagaatt tgccccatgc tgatgtactg cacccaggca ccggtaaact gcgcgtcggg
    37501 ctggcggaaa aactcaacaa tgatgcgaaa cgcgccgtag ccaatcagga acaagcctga
    37561 gacagctccc attgggcgcg gtttacgaat atacaggttg aggataataa acagcaccac
    37621 accttccagc agcagttcgt aaagctgtga tgggtggcgc ggcagcacac cgtaagtgtc
    37681 gaaaatggat tgccactgcg ggttggtttg cagcagcaaa atatcttctg tacgggagcc
    37741 tgggaacagc atggcaaacg ggaagttcgg gtcaacgcgg ccccacaatt caccgttaat
    37801 aaagttgccc agacgcccgg caccaagacc aaacggaatg agtggtgcga taaaatcaga
    37861 gacctggaag aaggaacgtt tagtacggcg ggcgaagata atcatcacca cgataacgcc
    37921 aatcaggccg ccgtggaaag acatgccgcc gtcccagaca cggaacagat acagcggatc
    37981 ggccataaac tgcgggaaat tgtagaacag aacataacca atacgtcccc cgaggaagac
    38041 gccgaggaag cccgcataga gtaagttttc aacttcattt ttggtccagc cactgcccgg
    38101 acgattcgcc cgacgtgttg ccagccacat tgcaaaaatg aaacccacca gatacatcag
    38161 gccgtaccag tgaagcgcca cgggtcctat tgagaaaatg accggatcaa actccggaaa
    38221 atgcagatag ctactggtca tctgtcacca caagttcttg ttatttcgct gaaagagaac
    38281 agcgattgaa atgcgcgccg caggtttcag gcgctccaaa ggtgcgaata atagcacaag
    38341 gggacctggc tggttgccgg ataccgttaa aagatatgta tatgatccgc gctataaccc
    38401 tccgcgaatc agcccgccca tgccgcgacg ctccataaag gctgcaacct gatgacgaac
    38461 ttcggtcgcc agttgcgctt ccagactacg ctgcgcaaga ttttctgctt cggcaaaatc
    38521 aatgcgccgc agcaggtatt ttacccgcgc tacagaacgt ccgttcatag acagatggcg
    38581 atacccaagc ccaatgagga ttgccacgca catgggatcg cccgccattt caccgcacaa
    38641 acggagatcg ataccatgta tttccgcttc ccgggcgatc atcgccagag ctcgtaacat
    38701 tgcaggatga agactgtcgt aaatgttcgc cacccgggta ttgttgcgat caacggccag
    38761 aatgtattga gtcagatcgt tggtgccaac agagatgaaa tcgacccgct ttgccagatg
    38821 cggcagcata aataccattg acggcacttc cagcatgatg ccgatacgtg gtttgggaat
    38881 ttcgtaaccg atcatctcct cgacttcacg tccggcacgt tcaatcaggc ggcgtgcttc
    38941 gtcaacttca tcgaggctgg tgaccatcgg caacagaata ttcaggttgc ccgtagcggc
    39001 attagcacgc agcatcgccc gcacctggat caagaagatc tccggctgat cgagcgtaat
    39061 gcgaatccca cgccaaccca ggcatggatt ctcttcgctg atcggcatgt aaggcagctg
    39121 cttatctgct ccgacatcca gcgtacgcaa ggtgacgggt ttatcattga acatttgcag
    39181 catcccctga tactgcgcca cctgttcttc ttccgacggg aaaccacttt gcagcatgaa
    39241 tgggatttca gtgcgataaa gaccgatgcc atcaatacgg ctgcccagtt tttcttcatg
    39301 ttccgggctt aaaccagcat tgagcatgac ttttatacgc tcaccgcttt ttaactgcgc
    39361 gggtaaattg acgtcatctt ccgccagacg gctaagctca atctcttcac taattagccg
    39421 ctgatattct tgcagcagta ccggctccgg atcgaccagc aattcaccgc gatagccatc
    39481 aacgatcagc gtcctacgat gcagcaccga aggctgaata tccgcgccca tcacggtagg
    39541 gatccccagc gcacgtacca tgatcgcagc atgggagttg gctgcgccat ctcgcacgac
    39601 aacaccgact aagcgatcct ggggcagctc agcaagagtt gtcgctgaca gttcatctgc
    39661 caccagaatg aaacgttccg gccaggcgtt cggcccttga ttagcgtcat caagatgaaa
    39721 cagcaatcgc tgacccagcg cacgtaaatc gccagcccgc tctttgagat agttatcgct
    39781 tagcgcggca aactgttcgg caaatttttc aatgaccgtt tttaccgccc actctgccac
    39841 cgagccttta tcaacctcgg caaacaattc gcgacgcagc cgggtgtccg aaagcaggtg
    39901 cgagtaaaga tcgaaaatag ccgccgtttc tttttgtgct ccggcggcaa agcgtttgct
    39961 gtagcggcga aactcgtttg ccgcttcttc cagcgccccg gtcagtcgtt cgcgttccag
    40021 agccggatcc agcgttgatg cctgatacac ctgttccatt aaaggtaacg tggcatcctg
    40081 ccagccttcg gcaatcgcca caccaggtgc tgccggtaat gcgcggattc gcgtctggcg
    40141 atattgccca aacagggcag tcaactgcga ctgagaaaga atagctgcca tctgggtggc
    40201 aagcgtcacc aggaaggatt cttcactttc gtcatactgg cgcaactctc gttgctgtac
    40261 caccagtaca ccaagcaact ggcgacgttg aataattggt acgcctaaaa acgcgcggaa
    40321 acgttcttct tttacggagg ggatgtattt gaagctgggg tgcttttgcg catctgcaag
    40381 gtttatcggt tccgccagcc tgccaaccag accgacgatc ccttcatcaa acgcgagcgt
    40441 tacagtgcga ccgcgtggtt ttttcagccc ccgggtcgcc atcaggtagt aacaacgtcg
    40501 atcatgatcg gccaggtaga ccgaacagac ctcggtatcc atcgcaagac agatgtcggt
    40561 aaccagaata tttaacgcct cattcaggcg tggtgcgctg gctacctttt cgactatttc
    40621 gcgcaggcga gtgagcataa ttggcgtgac ttaacctctt ttacgtcgat aagcagatgc
    40681 gttttgtggt tttggcgtat tttcctgcag tgacatcacc acactcgcga actctttcat
    40741 taccctacgg tagacatcac gtttaaatga caccacctgt ctgaccggat accagtaact
    40801 tacccatcgc cagccgtcaa actctggtgt actgctggtt tgcatattga tttctgcatc
    40861 gccgcttacc agctgcaaga gaaaccattt ttgtttttgg ccgatacaaa ccggcttcgt
    40921 gtcccaacgc accaaacgtt tcggtaattt gtagcgcaac cagttacgcg ttgaagcaag
    40981 gattcgaacg tctttgcggc ttaatcctac ttcttcaaac aattcacggt acatcgcctg
    41041 ctctgcggat tctccggggt tgattccgcc ttgcggaaat tgccaggagt gctgaccaaa
    41101 tcgccgggcc cacattacct gcccctggcg attacaaatc acgataccta cgtttgggcg
    41161 gtagccatcg tcatcaatca ccggactacc tcaaaataaa gctttatata cgaatgattg
    41221 tttcatactc caggaagacg gtaaaccact ctctgcaggg cattacacac taataacaat
    41281 tgaataactc acagttatgt gcagagttat aaacagagga aggggtggat agccgttttt
    41341 attcaccttt tctgtggata gagttgtgaa gaactacgga attactacgg gaaaacccgg
    41401 agcattctga ataagcccga tatagacatt ttaaaatatt cattattagt caatgcatta
    41461 catgtaattt gagtaatgaa aaagagtaat tcgtgaccca ggtcacacct ctcatttacg
    41521 ggttggcgaa agatcaacca atgccgtatt tatccacaga atgagccact aagttaagca
    41581 ctgaaccact aaaaactgga gtttcgtcgc acgtcaaggc tgtaaatgaa aacagtagtg
    41641 gaggtttttc acagttatcc cagctttctg tggataacat ggtgtaagat cctgtttatt
    41701 ttcagtgacc agatttggaa aacctattgc agtgttgcgc aactcgatta ccggcagcct
    41761 aaaaagcaat ataaatcaga gaattgaaca acacatgtgg aaaaagttac actgcgaata
    41821 ttcggcacat aattgctgtt tgttttttaa ccaaggtatc atgacatgtc ccaacctcgc
    41881 ccactgctct ctcctcccga aactgaagaa cagttgttag cgcaagcaca gcaactttct
    41941 ggttatacat tgggagaact ggcggcactt gccgggctgg ttacgccaga gaatttaaaa
    42001 cgcgataagg gctggattgg cgtgttactg gagatctggc taggtgccag cgcagggagt
    42061 aaacctgagc aagattttgc tgctctgggc gtggaactta aaactatccc tgtggatagt
    42121 cttggtcgtc cgctggaaac aacattcgtt tgtgttgccc cgttaacggg taatagcggg
    42181 gtgacctggg aaaccagcca tgtgcgccac aagctcaaac gcgtactgtg gataccggtt
    42241 gaaggcgagc gcagcatccc gctggcgcag cgtcgcgtag gatcaccgtt gctgtggagc
    42301 ccgaatgaag aggaagaccg acagctacgc gaagactggg aagaattaat ggatatgatt
    42361 gttctcggtc aggttgagcg gatcaccgct cgtcacgggg aatatttaca gataagaccg
    42421 aaagcagcga atgcgaaagc gcttaccgaa gccattggtg cccggggcga acggattctg
    42481 acgctgccac gcggctttta tttgaagaag aatttcacca gtgcactact ggcccgtcat
    42541 tttctgatcc agtagccatc gctttgacct gccgctttcc gggcatataa ttaccgcttc
    42601 atttttttgg cagggctttt tagatgttat ttgcatggat aaccgatcct aacgcctggc
    42661 ttgcgctcgg tacgctgacg ctgctggaga tcgttcttgg gatcgacaat attattttcc
    42721 tttctctggt ggtggcaaag cttcccacag cacaacgtgc tcatgcgcgc cgtctggggt
    42781 tggcgggagc catggttatg cgtctggcgc tgctggcatc aatctcttgg gttacgcgcc
    42841 tgacaaatcc gctttttaca atattcagtc aggaaatttc tgcccgtgat ttgattctgc
    42901 ttctgggtgg cttgttcctt atctggaaag ccagcaagga aatccacgaa tccattgaag
    42961 gtgaagaaga agggctgaaa acacgcgttt catcattcct cggcgctatc gtgcagatta
    43021 tgctgctgga tattatcttc agcctcgact cggtgattac cgctgtgggt ctgtcagatc
    43081 acctgtttat tatgatggca gccgtggtga ttgccgtagg cgtgatgatg ttcgccgcgc
    43141 gctcgattgg tgattttgtc gaacgccacc cttcggtaaa aatgctggcg ctctctttcc
    43201 tgattctggt gggctttacc ctgattctgg aaagtttcga catccacgta ccgaaaggtt
    43261 acatctactt cgcgatgttc ttctctattg cggttgaaag cctcaacttg attcgcaaca
    43321 aaaagaatcc gctctgatac tccgtacgct ctcctgcagg agggcgtact cactcctttt
    43381 accctcacag tttaagattt tcctgctttc aagatatata acgtcggttt ataaacagac
    43441 tattatcata ggtaggccaa atgaggataa ccggatgaaa aaatgggcag taataatttc
    43501 tgcagtcgga ctggcgtttg ctgtttccgg gtgttccagt gattacgtca tggcgaccaa
    43561 agatggccgt atgattttga ccgatggaaa acctgaaatt gatgatgata ccgggctggt
    43621 gagttatcac gatcagcaag gtaacgcgat gcaaattaac cgtgatgatg tttcgcaaat
    43681 tattgaacgt taacaaataa ggtcagcatc cggctggcct taagattttt ctctttccct
    43741 tccctctgcc atttttatat tccttatgtc gtgattataa aaaggaaacg gctatgcaat
    43801 atcaccgtat accccacagt tcgctggaag tcagcacgct ggggcttggc acgatgacgt
    43861 ttggtgaaca gaacagcgaa gccgacgccc acgcacaact cgactatgcc gtcgctcagg
    43921 gcattaacct tatcgacgtt gccgaaatgt acccagtacc tccgcgcccc gaaacgcaag
    43981 ggttaaccga aacctacgtc ggcaactggc tggcgaaaca tggcagccgc gaaaagttaa
    44041 ttatcgcctc caaagtgagc ggaccgtcgc gcaataatga caagggcatc cgcccggatc
    44101 aggcgctgga tcggaagaat atccgcgaag cgctgcatga cagcctcaag cgcctacaga
    44161 ctgattacct cgatctttat caggtgcact ggccgcagcg cccgaccaac tgcttcggca
    44221 aactcggtta tagctggacg gattctgcgc ctgcggtttc gctgctggat acgctggacg
    44281 cactggcaga gtaccaacgc gcgggaaaaa ttcgttatat cggcgtgtcg aacgaaactg
    44341 catttggcgt aatgcgctac ctgcatctgg cggacaaaca cgatctgccg cgtattgtca
    44401 ccattcagaa cccttacagt ctgttaaacc gcagttttga agtaggtctg gcagaagtca
    44461 gccagtatga aggggtcgaa ctgctggcct attcgtgcct gggtttcggc acgctgaccg
    44521 ggaaatatct caatggtgca aaacccgctg gcgcacgtaa tacgctcttt agtcggttca
    44581 cccgctatag cggtgagcaa acgcaaaaag ccgtcgcggc gtatgttgat atcgccagac
    44641 gtcatggcct tgatcctgct cagatggcgc tcgcgtttgt acgccgtcaa ccgtttgttg
    44701 ccagcactct gctgggcgca accacgatgg atcagctgaa aactaacatc gaaagtttgc
    44761 atctggagtt aagcgaagac gtattagctg aaattgaagc ggtacatcag gtttatactt
    44821 atcctgcacc ataaagatta acgccggtta aaaccggcgt taaatattaa tgacggcgct
    44881 gccagatcca cagcgccgtt attgccagcg caaatagcgc accgaagcca atgccaatgg
    44941 gcacgaccgg gatgcctacc attaccgcca gcgagtaaat gcccagcatc aacaacatgg
    45001 cgctgttttc gccaaggttt tgtactgcaa tcgcattccc cgccccgacg ctttttttac
    45061 cccgctcctg tagcaacgca ttgagcggaa cgacaaaaaa gccccccagc acgccaatca
    45121 gcatcaacaa ggcataggct ggcagcagct cgtgttgcag ggagaaaatc agtaccacca
    45181 cgccaatcaa aatcccggct ggcatacagc gtgacacggt ttccagcgta accagcttcg
    45241 ctgccgcacc tgcgccaacc acgataccaa tcgctaccat cgcgttgaga taggtcggcg
    45301 tagcgttatc ggtaatgccc agcgccaccg gtacccacag caccaacagg aaacgcagcg
    45361 tgacacccgc tccccagaat aaactggtgc ccaccagcga aaaacgcgtt tcaccattgc
    45421 accatagcga ggtgcaggca ttcaggaaac tgcgggtcat gttgatgaga ttccaggact
    45481 gccccggacg cgccgccgcc agtttgggaa tgtagatatt ggcaacgacc gcaccaccgt
    45541 aggccagtgc gcatgcggcc agggcgacca ggacatgcca gtcagccagc acaccaccgg
    45601 ctacggaacc gagtaaaatc gccgctatgg tagaagcttc cattaaaccg ttagctttta
    45661 ctaacttact acccgtggtt aattcgccga gaataccgta tttcgccggt gaataggctg
    45721 cagcaccaac acccaccagc gtatagccga gaaacggatt gataccaaag cagatactgg
    45781 ctgcgcccag cagcttcagg ccgttggcaa acatcatcac ccggcctttg gcgaagctat
    45841 ccgccacctg cccgacaaac ggcgccaaaa gaatgtaagc acctacaaac accatttgca
    45901 ggatgggctg gctccactcc ggatagaact gcgctttcag taacgccaga gtggcaaaca
    45961 gtagcgcgtt atcgccaaac gcagagagaa actgcgccac gataaccgct ttcatcccct
    46021 tcgaccacaa cgaagtgtta gtgtgcactg actcactcat cgtgttgttc cgcttcgtct
    46081 acccagcttt tcaacgtgac aaagtcaggt ttgccgctgc caagtaatgg catctgtttc
    46141 agatagcgaa tatcgcgcgg tacagcaagc tccggcacgc cgtgctcgcg ggcatactgt
    46201 tgcaacttat cgcgcgtcag ttcgttatct gtggtgaaaa gcaccagtgc ctcgcctttg
    46261 ctggcatcgc tcttaatcgc agtggcatgg actttatctg gcgaaacacc aagtgccagt
    46321 tgttccacca tttccagcga caccatttcg cctgcaattt tggcaaagcg ttttacgcgg
    46381 ccctgaatct gcacaaagcc ctgctcgtca aaacgcacaa tatcgccagt gtcataccag
    46441 ccgcgctcca tttcgccgcg aacattctcg gcggtgggca cttccagtac acctggcttc
    46501 tccacccgca gatagccgtt cattatgttc ggccctttca gttgcaggcg tccgccctct
    46561 tcgataccag ggaccgacaa caggcgtgca tccattcctg gtagaatacg ccctaccgta
    46621 ccgggtttcg ccgccatcgg tacgttgata gaaacgacag gcgcgcattc ggtcacaccg
    46681 tagccttcaa ggatgcgcag gccaaattta tcctgccaaa gctgtttggt actttcttgt
    46741 agtttttctg cgcctgccac cacatagcgt agacgataga agtcatacgg gttggcgaaa
    46801 cgcgcgtagt gaccgaggaa agtcgaggta ccgaacaaca cggtgcaact gcggtcatac
    46861 accaagtccg gcacaatgcg gtaatgcagc gggcttggat aaaggaacac ttctgcacct
    46921 gtaagcagtg gcgtaaacag gcctaccgtc agcccaaagg agtgaaacag cggtaatgcc
    46981 gacataaagc gatcgttggt ggtgaagtcg gcaatcgttt taatctgctc gacgttcgcc
    47041 agaatgcttt tatggctatg gacgacgcct tttggatggc cttcagaacc ggaggtaaag
    47101 aggatcagcg cctcttcttc cggctgctgt tttacctgcg ccaggcgcgg catcagcaaa
    47161 tgagcgaaga tccatacttt gtcggcagtg gtgacatctg cttttaaatc ttccagatag
    47221 acccagcgca cctgagtaag ttgctccggc agatgccaga gtttgccttt atcgagaaac
    47281 tggcaggaag tgaagatggt tttgatttca gccgccgtaa tagcactggt cagccctttt
    47341 accccggcag tgtagttcat cattgcggga atgcggcgac gcgcgatggc cccaaaaatc
    47401 actgccgcac tgatgcctgc attgggcagc attaagccga tgcgttcgcc ttcaacactg
    47461 tatttttcaa ggatgcgtcc aacaaacagc gttttcgtaa gcaatttgcg ataggagtct
    47521 ggggtaaagt tgacgtcttc gacacatttc ttcccggctc cgaagcggta cattgcactc
    47581 agtaaagatt cgtacagagt ttcacgcggg cgcaccgcca tacgcgcttc catcattatt
    47641 tgatgcagca tttcgccagc gattttgcga cggtcacggg cacgcggcgc atccggcatc
    47701 gccacctgcg ttggaggcaa aatatgcaga gtaatttgcg ggaacaagcg acgtttaacc
    47761 agacctttca ggcggctgaa gtgcgtaagt tccgcccctt caatacgcac aggaataacc
    47821 gttgcaccag acttcgccgc gacaaaaccc gcgccatcgt agattttcat cagcgagcct
    47881 gtagtggtga tgcgtccttc agggaaaatc accactggtc ggccctgttc caccagacgt
    47941 accagatgtt taatagccat aggttgcgtc gggtcgagag gaacaaagtc gataaatgat
    48001 ttcagccaac gcatatacca ctgttggctt attgaggtgt aaacggcaaa cactggacgc
    48061 acaggtaaaa acagtccaag caaaatgcca tcaataaaag agacgtgatt aggcgtaatt
    48121 agaacgcgct cgcccttcag tgcctgggtg tcacccgtaa cgcgaacgcg atacaaaaca
    48181 cggcacaaat ttcgaaaaaa gctaaaaagc attccaactc cctttgctct gattcagtaa
    48241 aagcgaatgg agggagatta cacgagataa agaacgcgag cgacagtaaa ttaggtgcga
    48301 aaaaaaacct gcgcatccgc gcaggttggt gcaagagaca gggtacgaag agcgtaccga
    48361 ataatctcac caatcaatac ctctgggatc ttgattgtgg tctgcacgac gactcttcgc
    48421 cagcgagaaa acgcaaagga atgaagggaa atgcaacgag gtgtgtaaat tgtcggttac
    48481 tgttacagat tgatgaccgg caaaaaaaaa cctgcgcatc tgcgcaggct ggtgtaattc
    48541 atgtgctcaa cccgaagttg acttcaccta tcaatacctc tggaatcacc actttagcaa
    48601 cctgaagcca aacgccacca gcggtcaatc gcaacagcgt ttcgcaaagt gtaaccaaag
    48661 gtttgcaatc tcttttttgt cttacttatt tgcgtttggc tcacattccc acgatgaaaa
    48721 cacgccaccc cttgaaccaa cgggcgtttt ccgtaacact gaaacaatgt aagcgtttac
    48781 ccactaaggt attttcatgg cgaccataaa ggatgtagcc cgactggcag gcgtttcagt
    48841 cgccaccgtt tcccgcgtca ttaataattc acccaaagcc agcgaagctt cccggctggc
    48901 tgtgcatagt gcaatggagt ctcttagcta tcacccgaac gccaacgccc gtgcgctggc
    48961 gcagcagacc actgaaacgg tcggtctgat cgttggtgat gtttccgatc cgtttttcgg
    49021 tgcaatggtg aaagcggtcg aacaggtggc ttatcacacc ggtaatttct tattgattgg
    49081 caacggttac cacaacgaac aaaaagagcg tcaggctatt gagcaactga tccgccatcg
    49141 ctgtgctgcg ttggtcgtcc atgccaaaat gatcccggat gccgatttag cctcattaat
    49201 gaaacaaatg cccggtatgg tgctgatcaa ccgtatcctg cctggctttg aaaaccgttg
    49261 tattgctctt gacgatcgtt acggtgcctg gctggcaacg cgtcatttaa ttcagcaagg
    49321 tcatacccgc attggttatc tgtgctctaa ccactctatt tctgacgccg aagatcgtct
    49381 gcaagggtat tacgatgccc ttgctgaaag tggtattccg gccaatgatc ggctggtgac
    49441 atttggcgaa ccagacgaaa gcggcggcga acaggcaatg accgagcttt tgggacgagg
    49501 aagaaatttc actgcggtag cctgttataa cgattcaatg gcggcgggcg cgatgggcgt
    49561 tctcaatgat aatggtattg atgtaccggg tgagatttcg ttaattggct ttgatgatgt
    49621 gctggtgtca cgctatgtgc gtccgcgcct gaccaccgtg cgttacccaa tcgtaacgat
    49681 ggcgacccag gctgccgaac tggctttggc actggcggat aatcgccctc tcccggaaat
    49741 tactaatgta tttagtccga cgctggtacg tcgtcattca gtgtcaactc cgtcgctgga
    49801 ggcaagtcat catgcaacca gcgactaacc gcagttaaag caattccagc gccagtaatt
    49861 cttcgatggt ctggcgacgg cgaatcaacc gcgcctgacc attatcaaac agaacttctg
    49921 gtaacagcgg acggctattg tagttggatg acattgatgc gccatatgcc cctgtatcat
    49981 gcagtaccag ataatcacct gccttcactt ccggcaaggc gcgggtttca acatttcccc
    50041 cttcctgctg ggtaaagaca tcgcccgatt cacataacgg cccggcgacg acggtttcca
    50101 ccgttggcgc gtgttccaga gaacgaccat cagctgccag ggcactgata tggtggtaac
    50161 taccgtacat tgccgggcgc atcagatcgt tgaacccggc atcaaccagc acaaagtggc
    50221 ggctacccat ttgtttgacg ctccgcacct gagtaattaa tacgccagac tgcgctacca
    50281 ggaagcgacc cggttcaatt tccagtttca cagggtggcc caaatggcga gcgatttgct
    50341 cacgcgcggc attccacaga ccataataat gttcggtatc aaccgcctct tcaccctgtt
    50401 gataaggaat agaaagccca ccgcccgcag aaatagcctg taaatcctga ccgaattcga
    50461 tgacctgacg caccatagca ccacacacct gctccagatg ggcataatca acgccagaac
    50521 caatgtgcat gtgaatgccg actagctgca gatgatgacg ttgtatcacg tccagtgcgg
    50581 cgggcagatc ggtgtaccag ataccgtgct tgctgttttc gccaccggta ttggtttttt
    50641 ggctatgtcc gtgaccaaac cccggattaa cgcgcagcca tacccgatgc cctggcgaaa
    50701 cctggcccag ttggtcgagc atatcaacag aacccgcatt caccggaatt tgcaattcac
    50761 tgacgcgttc aagcgtcgcc tgatcgataa catctgccgt aaaaacaata tcatcggggt
    50821 gcgtttgcgg attgtaaccc gccgccaacg cacgctctat ttcgcctaac gagacggaat
    50881 ccactttcac gccctgctca cgcattaagc gcaaaatatg aatattggaa caggctttct
    50941 gtgcaaagcg caccacttca aactgtttca gcgctgcaat ctgccgacga ataatttgcg
    51001 catcgtagac ccacaccggg cagccaaatt cagcgggcaa acgcagcaga ttttcggcgg
    51061 tgagatcggt atcggtgctg aacagtgaat gtggcataac aaactccaga tgagtgcttt
    51121 tttatgatta cgccacatca taaaaagaat aaaaaatatc gatttatgtc gagtctatgc
    51181 aaaaatgata tggattaccg gattgcgaga gagcgctaat ggccgccgtt aacttacgtc
    51241 atattgaaat ttttcatgcg gtaatgaccg ccggaagcct gactgaggca gcacacctgc
    51301 tacacacctc acagccaacc gtcagccgcg aactggcgcg ctttgagaag gtgatcgggc
    51361 tgaaattgtt tgagcgcgta cgtggacgat tacatcctac cgtgcaagga ctgcgtctgt
    51421 ttgaagaagt gcaacgatcc tggtacggac tggatcgcat tgtcagcgcc gcagaaagtc
    51481 tgcgcgagtt tcgccaggga gaactgtcta ttgcctgcct gccggtcttt tcgcaatctt
    51541 ttttaccgca gctcctgcaa ccctttctgg cacgttatcc cgatgtcagc ttaaatatcg
    51601 tgccccagga atcaccgcta cttgaagagt ggctctcggc ccagcgtcat gatttaggac
    51661 tcactgagac actccatacg cctgcgggaa cagaacgtac cgaattactc tctttagatg
    51721 aagtgtgtgt gttacctccg ggtcatccgc tggcggtaaa aaaggtatta acgccggatg
    51781 attttcaggg tgagaattac atcagccttt cccgtactga cagctatcgc cagttgctgg
    51841 atcagctatt tactgaacat caggttaaac gacgcatgat cgtagaaacc cacagcgccg
    51901 cgtcagtctg cgcaatggta cgggcggggg taggtgtttc ggtggttaac ccgctcaccg
    51961 cactagatta tgcggcaagc ggtttagtgg tgcggcggtt cagtattgcg gttccgttca
    52021 ccgtcagcct gatccgcccc ctgcaccgcc cgtcatcagc gctggttcag gcgtttagtg
    52081 ggcatttaca agcggggtta ccgaaactag tcacttctct tgacgctatt ttgtcgtcag
    52141 ctacgacagc ataaaagcga cagcatcctc ggcatggatc gccgcggtat caaacacagg
    52201 caaaacactg cgctcttctg gcaccagtaa accaatttct gtgcagccaa aaatgacgcc
    52261 ctgtgcgccc tgttctgcaa ggcgagcaat cacttgcgca taataagcgc gtgacgcttc
    52321 ggtaaattgc cccagacaca gttcttcaaa aataatctga ttaattttcg cccgttcatc
    52381 cgcttcagga ataagacaat tgatggaaaa ttgttccgtc agccgcccgc gataaaaatc
    52441 ctgttccatg gtgtaacgcg tacccagcag cgccacacga gtcattccgg ccccggtaat
    52501 tgcacgtccg gtggcatccg caatgtgtaa gaaaggcaga gtgcaacgtg actcaatggc
    52561 atccgccact ttatgcatcg tattggtaca taacacaata ccttctgcgc ccgcccgctg
    52621 taagccaagc gccgcctcag ccagaatgtc cccggtttta tcccattccc cgcgacgctg
    52681 gcactcttct atttcatgaa aatcgacgct atgtagcagc atttgcgcag agtgaagccc
    52741 accaagccgc tgtttaatgc cttcatttat cagacgatag taaggaatgg tggattccca
    52801 gctcattcct cccagcaaac caattgtttt cattcctctc tcctgatgtg tgttaccgca
    52861 acaaggctga ataaggaacg agacaaacgc ctcaacggcc aggtgcccaa tctctattaa
    52921 cgaaaaaagg gccggatgta cagcacatcc ggcccgtgaa atcagacgcc gatatttctc
    52981 aacttctcgc ctgccatcag tttgcgttcg atatgttcca gcgtgacatt tttggtttcc
    53041 ggaatgagcc agaaagtaat gcccacaaac gcaatgttca gcgcagtgta gagccagaac
    53101 gtaccggcag cgccaatgct atcaagcagt gtcaggaagg tcgcgccgat aatcatattc
    53161 gacacccagt ttgtcgtcgt cgaacaggta ataccgaaat cgcggcattt cagcggctga
    53221 atttcagagc acaggatcca caccactggc gcggcgctca tcgcataacc ggcaatacac
    53281 atcatcgtca tgccaacaga gagccaagac aagccactgg aagccgtacc gttatcaaac
    53341 tgcatcaggc aatagcccag caccagagtg cctaacgcca tcacgctgaa accaattttc
    53401 agagccggtt tacgacctgc tttatctacc gtaaagaccg caatgaaggt cgcgaacata
    53461 aaggtcagtc ccacgaccag agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc
    53521 attttgaaga tacgcggcgc gtagtacatg atgatgttca taccggtaaa ctgctgcatc
    53581 gcctgcaaca acataccgag aaacacagca cgacggacgt tacggttgat cttaaacagt
    53641 gcccaaccgc cctgttttaa cttcaggctt tcacgaattt cgttgagttc ttctcgcgct
    53701 ttttccgacg tatcgcgcag catacgcaac acttcttccg cctcaatatg acgccccttt
    53761 tccgccagcc agcgcgggct atttggcagg aagaccacca gaataatcag cagaactgct
    53821 ggtaaagcaa gaacccccaa cattgcgcgc cagttaccgc tataactgaa cgctgtatcg
    53881 gataaaaacg ccagcacgat gccgagtgtg accatcaact ggtacatact gatcatctta
    53941 ccgcgaacgt tttcacttgc catttcagaa agatacagag gagcggtgta agacgcgatc
    54001 ccgacagcaa tgcccagcac cacacgagcg gcgattaaca tctctacgct ggtcgcaaaa
    54061 gcggacccta tagaaccgag tacaaacagg atggcccccg ccatcaggct gtatttacgc
    54121 cccaggcgga acgacagcca accattaaac agcgcaccaa ttgctgcgcc gagcatcatg
    54181 ctactaacca cccattcctg caaacgactg gtcagcacaa agtgatcggt aatgaacggc
    54241 aacgctccgg cgattacgcc gatatcaaga ccaaataaca atcctgcgac cgcagcagct
    54301 accgaaacaa acatattcat acgccgcgta tcacgcaaag aacgtggcgt taaagcagat
    54361 tccgtattga tagtaaccat tttttcctgc cagcagagag taagacatag tgaaaaaata
    54421 cgtgaacaat tcacacaggt gtcaggtcgg aaacagcata aatatggatt aaattgctgc
    54481 gacatgccgt tatgtgatgg atattccaat tttaaaatta agttgaatta ttgagattat
    54541 tattaaccac ctaattttaa agcagataaa attcataaag ttcattaatt gataattgat
    54601 atggattatt tcataaccat gatatggatt atgatg
//
LOCUS       DBKEKI010000011        52173 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.011, whole genome shotgun sequence.
ACCESSION   DBKEKI010000011 DBKEKI010000000
VERSION     DBKEKI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.011"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="ACUQPX_000631"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="ACUQPX_000631"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HHV9055424.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2002)
                     /gene="pcnB"
                     /locus_tag="ACUQPX_000632"
     CDS             complement(605..2002)
                     /gene="pcnB"
                     /locus_tag="ACUQPX_000632"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HHV9055425.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(2062..2958)
                     /gene="gluQRS"
                     /locus_tag="ACUQPX_000633"
     CDS             complement(2062..2958)
                     /gene="gluQRS"
                     /locus_tag="ACUQPX_000633"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HHV9055426.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="ACUQPX_000634"
     CDS             complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="ACUQPX_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HHV9055427.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="ACUQPX_000635"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="ACUQPX_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HHV9055428.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="ACUQPX_000636"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="ACUQPX_000636"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HHV9055429.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="ACUQPX_000637"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="ACUQPX_000637"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HHV9055430.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7606..10140
                     /gene="mrcB"
                     /locus_tag="ACUQPX_000638"
     CDS             7606..10140
                     /gene="mrcB"
                     /locus_tag="ACUQPX_000638"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HHV9055431.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10360..12603
                     /gene="fhuA"
                     /locus_tag="ACUQPX_000639"
     CDS             10360..12603
                     /gene="fhuA"
                     /locus_tag="ACUQPX_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HHV9055432.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKNGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12654..13451
                     /gene="fhuC"
                     /locus_tag="ACUQPX_000640"
     CDS             12654..13451
                     /gene="fhuC"
                     /locus_tag="ACUQPX_000640"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HHV9055433.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13451..14341
                     /gene="fhuD"
                     /locus_tag="ACUQPX_000641"
     CDS             13451..14341
                     /gene="fhuD"
                     /locus_tag="ACUQPX_000641"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HHV9055434.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14338..16320
                     /gene="fhuB"
                     /locus_tag="ACUQPX_000642"
     CDS             14338..16320
                     /gene="fhuB"
                     /locus_tag="ACUQPX_000642"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HHV9055435.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="ACUQPX_000643"
     CDS             complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="ACUQPX_000643"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HHV9055436.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEESGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17801..17848
                     /gene="yadX"
                     /locus_tag="ACUQPX_000644"
     CDS             17801..17848
                     /gene="yadX"
                     /locus_tag="ACUQPX_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HHV9055437.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17860..19281
                     /gene="clcA"
                     /locus_tag="ACUQPX_000645"
     CDS             17860..19281
                     /gene="clcA"
                     /locus_tag="ACUQPX_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HHV9055438.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19305..19370
                     /gene="yadW"
                     /locus_tag="ACUQPX_000646"
     CDS             19305..19370
                     /gene="yadW"
                     /locus_tag="ACUQPX_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HHV9055439.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19363..19707
                     /gene="erpA"
                     /locus_tag="ACUQPX_000647"
     CDS             19363..19707
                     /gene="erpA"
                     /locus_tag="ACUQPX_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HHV9055440.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19754..20377)
                     /locus_tag="ACUQPX_000648"
     CDS             complement(19754..20377)
                     /locus_tag="ACUQPX_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HHV9055441.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="ACUQPX_000649"
     CDS             complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="ACUQPX_000649"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HHV9055442.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="ACUQPX_000650"
     CDS             complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="ACUQPX_000650"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HHV9055443.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21990..23507
                     /gene="dgt"
                     /locus_tag="ACUQPX_000651"
     CDS             21990..23507
                     /gene="dgt"
                     /locus_tag="ACUQPX_000651"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HHV9055444.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRINLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23637..25061
                     /gene="degP"
                     /locus_tag="ACUQPX_000652"
     CDS             23637..25061
                     /gene="degP"
                     /locus_tag="ACUQPX_000652"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HHV9055445.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25216..26372
                     /gene="cdaR"
                     /locus_tag="ACUQPX_000653"
                     /pseudo
     CDS             25216..26372
                     /gene="cdaR"
                     /locus_tag="ACUQPX_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26461..26847)
                     /locus_tag="ACUQPX_000654"
     CDS             complement(26461..26847)
                     /locus_tag="ACUQPX_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HHV9055446.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="ACUQPX_000655"
     CDS             complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="ACUQPX_000655"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HHV9055447.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="ACUQPX_000656"
     CDS             complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="ACUQPX_000656"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HHV9055448.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVCELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30750..31544)
                     /gene="map"
                     /locus_tag="ACUQPX_000657"
     CDS             complement(30750..31544)
                     /gene="map"
                     /locus_tag="ACUQPX_000657"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HHV9055449.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31912..32637
                     /gene="rpsB"
                     /locus_tag="ACUQPX_000658"
     CDS             31912..32637
                     /gene="rpsB"
                     /locus_tag="ACUQPX_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HHV9055450.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32895..33746
                     /gene="tsf"
                     /locus_tag="ACUQPX_000659"
     CDS             32895..33746
                     /gene="tsf"
                     /locus_tag="ACUQPX_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HHV9055451.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33893..34618
                     /gene="pyrH"
                     /locus_tag="ACUQPX_000660"
     CDS             33893..34618
                     /gene="pyrH"
                     /locus_tag="ACUQPX_000660"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HHV9055452.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPIATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34910..35467
                     /gene="frr"
                     /locus_tag="ACUQPX_000661"
     CDS             34910..35467
                     /gene="frr"
                     /locus_tag="ACUQPX_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HHV9055453.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35559..36755
                     /gene="ispC"
                     /locus_tag="ACUQPX_000662"
     CDS             35559..36755
                     /gene="ispC"
                     /locus_tag="ACUQPX_000662"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HHV9055454.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36944..37702
                     /gene="ispU"
                     /locus_tag="ACUQPX_000663"
                     /gene_synonym="uppS"
     CDS             36944..37702
                     /gene="ispU"
                     /locus_tag="ACUQPX_000663"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     ditrans,polycis-undecaprenyl-diphosphate synthase
                     [(2E,6E)-farnesyl-diphosphate specific] activity [Evidence
                     IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HHV9055455.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37715..38572
                     /gene="cdsA"
                     /locus_tag="ACUQPX_000664"
     CDS             37715..38572
                     /gene="cdsA"
                     /locus_tag="ACUQPX_000664"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HHV9055456.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38584..39936
                     /gene="rseP"
                     /locus_tag="ACUQPX_000665"
     CDS             38584..39936
                     /gene="rseP"
                     /locus_tag="ACUQPX_000665"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HHV9055457.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMFVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39966..42398
                     /gene="bamA"
                     /locus_tag="ACUQPX_000666"
     CDS             39966..42398
                     /gene="bamA"
                     /locus_tag="ACUQPX_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HHV9055458.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42520..43005
                     /gene="skp"
                     /locus_tag="ACUQPX_000667"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42520..43005
                     /gene="skp"
                     /locus_tag="ACUQPX_000667"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HHV9055459.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43009..44034
                     /gene="lpxD"
                     /locus_tag="ACUQPX_000668"
     CDS             43009..44034
                     /gene="lpxD"
                     /locus_tag="ACUQPX_000668"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HHV9055460.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGTNAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44139..44594
                     /gene="fabZ"
                     /locus_tag="ACUQPX_000669"
     CDS             44139..44594
                     /gene="fabZ"
                     /locus_tag="ACUQPX_000669"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HHV9055461.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44598..45386
                     /gene="lpxA"
                     /locus_tag="ACUQPX_000670"
     CDS             44598..45386
                     /gene="lpxA"
                     /locus_tag="ACUQPX_000670"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HHV9055462.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45386..46534
                     /gene="lpxB"
                     /locus_tag="ACUQPX_000671"
     CDS             45386..46534
                     /gene="lpxB"
                     /locus_tag="ACUQPX_000671"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HHV9055463.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46531..47127
                     /gene="rnhB"
                     /locus_tag="ACUQPX_000672"
     CDS             46531..47127
                     /gene="rnhB"
                     /locus_tag="ACUQPX_000672"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HHV9055464.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47164..50646
                     /gene="dnaE"
                     /locus_tag="ACUQPX_000673"
     CDS             47164..50646
                     /gene="dnaE"
                     /locus_tag="ACUQPX_000673"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HHV9055465.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLTFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEQEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50659..51618
                     /gene="accA"
                     /locus_tag="ACUQPX_000674"
     CDS             50659..51618
                     /gene="accA"
                     /locus_tag="ACUQPX_000674"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HHV9055466.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPVITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51717..>52173
                     /locus_tag="ACUQPX_000675"
     CDS             51717..>52173
                     /locus_tag="ACUQPX_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HHV9055467.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGH"
BASE COUNT        12244 a        12852 c        14517 g        12560 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggaactttcc agccaaaaag
     2341 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga gcttgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tatttgcgct cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcggatt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagcctcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cactgtatgg cgcgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc taggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccgg acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaaccctc tccctcaagg
     7501 gagaggggac cgatcgagca caaattttga gagatatctt cttctgtctt gtaacagaag
     7561 aacagaaaat cgggcttttg cgcctgaata ttgcggagaa aaagcatggc cgggaatgac
     7621 cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa ggtaagccgt
     7681 cgtcgttacg aagatgacga tgattacgac gattatgatg actatgagga tgaagaaccg
     7741 atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa acgcggctgg
     7801 ctatggctac tgctaaaact ggctatcgtt tttgccgtgc tgatcgccat ttacggcgtt
     7861 tatctcgatc aaaaaattcg tagccgtatt gatggcaagg tctggcaact gcccgcggca
     7921 gtttatggcc gaatggtcaa ccttgaacca gatatgacca tcagcaagaa cgaaatggtg
     7981 aagctgctgg aggcaacaca gtatcgtcag gtgtcgaaaa tgacccgtcc tggcgaattt
     8041 accgtgcagg ccaacagcat tgagatgatc cgccgtccgt ttgatttccc ggacagtaaa
     8101 gaagggcagg tgcgtgcgcg tctgaccttt gatggtgatc atctggcgac catcgtcaat
     8161 atggagaaca accgtcagtt cggtttcttc cgtcttgatc cgcgtctgat caccatgatc
     8221 tcctcgccaa acggtgagca gcgtttgttt gtgccgcgca gtggtttccc tgatttgctg
     8281 gtggatactt tgctggcgac agaagaccgt catttttacg agcatgatgg aatcagtctc
     8341 tactccattg ggcgtgcggt gctggcaaac ctgaccgcag gtcgcacggt gcagggtgcg
     8401 agtacgctga cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg ttcttactgg
     8461 cgtaaagcga acgaagctta catggcgctg atcatggacg cgcgttacag caaagaccgt
     8521 attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa cgaaatccgc
     8581 ggcttcccgc tggcaagctt gtattacttt ggccgcccgg tagaagagct aagcctcgac
     8641 cagcaggcgc tgttagtcgg tatggtgaaa ggggcgtcca tctacaaccc gtggcgtaac
     8701 ccgaaactgg cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca gcaacagatt
     8761 attgatcaag aactctatga catgttgagt gcccgtccgc tgggggttca gccgcgtggt
     8821 ggggtgatct ctcctcagcc agcctttatg cagctggtgc gtcaggagtt gcaggcaaaa
     8881 ctgggcgata aggtaaaaga tctctccggc gtgaagatct tcactacctt tgactcggtg
     8941 gcccaggacg cggcagaaaa agccgccgtg gaaggcattc cggcactgaa gaaacagcgt
     9001 aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg tgaagttcgt
     9061 gcgatggtcg gaggttctga gccgcagttt gcgggctaca accgtgcgat gcaggcgcgt
     9121 cgttcgattg gttcccttgc aaaaccagcg acttatctga cggctttaag ccagccgaaa
     9181 atctatcgcc tgaacacgtg gattgccgat gcgccaatcg cgctgcgtca gccgaatggc
     9241 caggtctggt caccgcagaa cgatgaccgt cgttatagcg aaagcggcag agtgatgctg
     9301 gtggatgcgt tgacacgttc gatgaacgtg ccgacggtaa atctggggat ggcgctgggg
     9361 ctgcctgcgg ttacggagac ctggatcaaa ctgggcgtac cgaaagatca gttgcatccg
     9421 gttccggcaa tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc gcaggcattc
     9481 cagaccatcg ccagcggtgg taaccgtgca ccgctttctg cactgcgttc ggtaatcgcg
     9541 gaagatggca aggtgctgta tcagagcttc ccgcaggcag aacgcgctgt tccggcgcag
     9601 gcggcgtatc tgacactatg gaccatgcag caggtggtac aacgtggtac gggtcgtcag
     9661 cttggggcga aatacccgaa cctgcatctg gcagggaaaa cagggactac caacaataac
     9721 gtagatacct ggtttgcagg cattgacggc agcacggtga ccatcacctg ggtaggccgt
     9781 gataacaacc agccgaccaa actgtatgga gccagcgggg caatgtcgat ttatcagcgt
     9841 tatctggcta accagacgcc aacgccgctg aatcttgttc cgccagaaga tattgcagat
     9901 atgggcgtgg actacgacgg caactttgtt tgcagcggtg gcatgcgtgt cttgccggtc
     9961 tggaccagcg atccgcaatc gctgtgccag cagagcgaga tgcagcagca gccgtcaggc
    10021 aatccgtttg atcagtcttc tcagccgcag caacagccgc aacagcaacc tgctcagcaa
    10081 gagcagaaag acagcgacgg tgtagccggt tggatcaagg atatgtttgg tagtaattaa
    10141 catctaagcg tgaaataccg gatggcgagt tgccatccgg taaaataaca tcccatctaa
    10201 gatattaacc ctttcttttc atctggttgt ttattaaccc ttcaggaacg ctcagattgc
    10261 gtaccgcttg cgaacccgcc agcgtttcga atattatctt atatttataa taatcattct
    10321 cgtttacgtt atcattcact ttacatcaga gatataccaa tggcgcgttc caaaactgct
    10381 cagccaaaac actcactgcg taaaatcgca gttgtagtag ccacagcggt tagcggcatg
    10441 tctgtttatg cacaggcagc ggttgaaccg aaagaagaca ctatcaccgt taccgctgca
    10501 cctgcgccgc aagaaagcgc atgggggcct gctgcaacta ttgcggcgcg acagtcagct
    10561 accggcacta aaaccgatac gccgattcaa aaagtgccac agtctatttc tgttgtgacc
    10621 gccgaagaga tggcgctgca tcagccgaag tcggtaaaag aagcgcttag ctacacgccg
    10681 ggtgtctctg ttggtacgcg tggcgcatct aacacctatg accacctgat cattcgcggt
    10741 tttgcggcag aaggccaaag ccaaaataac tatctgaatg gcctgaagtt gcagggcaac
    10801 ttctataacg atgcggtcat tgacccgtat atgctggaac gcgctgaaat tatgcgtggc
    10861 ccggtttccg tgctttacgg taaaagcagt cctggcggtc tgttgaatat ggtcagcaag
    10921 cgtccgacca ccgaaccgct gaaagaagtt cagtttaaag ccggtactga cagcctgttc
    10981 cagactggtt ttgactttag cgatgcgttg gatgatgacg gtgtttactc ttatcgcctg
    11041 accggtcttg cgcgttctgc caatgcccag cagaaagggt cagaagagca gcgttatgct
    11101 attgcaccgg cgttcacctg gcgtccggat gataaaacca atttcacctt cctttcttac
    11161 ttccagaacg agccggaaac cggttattac ggctggttgc cgaaagaggg aaccgttgag
    11221 ccgctgccga acggtaagcg tctgccgaca gactttaatg aaggggcgaa gaacaacacc
    11281 tattctcgta atgagaagat ggtcggctac agcttcgatc acgaatttaa cgacaccttt
    11341 actgtgcgtc agaacctgcg ctttgctgaa aacaaaacct cgcaaaacag cgtttatggt
    11401 tacggcgtct gctccgatcc ggcgaatgct tacagcaaac agtgtgcggc attagcgcca
    11461 gcggataaag gccattatct ggcacgtaaa tacgtcgttg atgatgagaa gctgcaaaac
    11521 ttctccgttg atacccagtt gcagagcaag tttgccactg gcgatatcga ccacaccctg
    11581 ctgaccggtg tcgactttat gcgtatgcgt aatgacatca acgcctggtt tggttacgac
    11641 gactctgtgc cactgctcaa tctgtacaat ccggtgaata ccgatttcga cttcaatgcc
    11701 aaagatccgg caaactccgg cccttaccgc attctgaata aacagaaaca aacgggcgtt
    11761 tatgttcagg atcaggcgca gtgggataaa gtgctggtca ccctaggcgg tcgttatgac
    11821 tgggcagatc aagaatctct taaccgcgtt gctgggacga ccgataaacg tgatgacaaa
    11881 cagtttacct ggcgtggtgg tgttaactac ctgtttgata atggtgtaac accttacttc
    11941 agctatagcg aatcgtttga accttcttcg caagttggga agaatggtaa tattttcgca
    12001 ccgtctaaag gtaagcagta tgaagtcggc gtgaaatatg taccggaaga tcgtccgatt
    12061 gtagttactg gtgccgtgta taatctcact aaaaccaaca acctgatggc ggaccctgag
    12121 ggttccttct tctcggttga aggtggcgag atccgcgcac gtggcgtaga aatcgaagcg
    12181 aaagcggcgc tgtcggcgag tgttaacgta gtcggttctt atacttacac cgatgcggaa
    12241 tacaccaccg atactaccta taaaggcaat acgcctgcac aggtgccaaa acacatggct
    12301 tcgctgtggg ctgactatac cttctttgac ggtccgcttt caggtctgac gctgggcacc
    12361 ggtggtcgtt atactggctc cagctatggt gatccggcta actcctttaa agtgggaagt
    12421 tatacggtcg tggatgcgtt agtgcgttat gatctggcgc gagtcggcat ggcgggctcc
    12481 aacgtggcgc tgcatgttaa caacctgttc gatcgtgaat acgtcgccag ctgctttaac
    12541 acttatggct gcttctgggg cgcagaacgt caggtcgttg caaccgcaac ctttcgtttc
    12601 taatttctct tttggggcac ggatttccgt gcccatttca caagttggct gttatgcagg
    12661 aatacacgaa tcattccgat accacttttg cactgcgtaa tatctccttt cgtgtgcccg
    12721 ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg accggtctga
    12781 ttggtcacaa cggttctggt aaatccactc tgctcaaaat gcttggtcgt catcagcctc
    12841 cgtcagaagg ggagattctt cttgatgccc agccgctgga aagctggagc agcaaagcgt
    12901 ttgcccgcaa agtggcttat ttgccgcagc agcttcctcc ggcagaaggg atgaccgtgc
    12961 gtgaactggt ggcgattggt cgttacccgt ggcatggcgc gctggggcgc tttggtgcgg
    13021 cagatcgcga aaaggtcgag gaagctatct cgctggttgg cttaaaaccg ctggcgcatc
    13081 ggctggtcga tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc atgctggtgg
    13141 cgcaggatag ccgttgtctg ttgctcgacg aaccgacttc ggcgctggat atcgcccacc
    13201 aggttgatgt gctggcgctg gtgcaccgtt taagtcagga acgtggcctg acggtcattg
    13261 ccgtgttgca cgatatcaat atggcggcac gctactgtga ttatctggtc gccctgcgca
    13321 gtggtgaaat gattgctcag ggaacgcctg cggaaattat gcgcggcgaa accctcgaaa
    13381 tgatttatgg catcccgatg ggtattttgc cgcatccggc gggtgctgca cctgtgagtt
    13441 ttgtttattg atgagcggct tacctcttat ttcgcgccgt cgactgttaa cggcgatggc
    13501 gctttctccg ttgttatggc agatgaatac cgcccacgcg gcggctattg accccaatcg
    13561 tattgtggcg ctggagtggt tgccggtgga attactgctg gcgctcggca tcgtgcctta
    13621 cggcgtggcg gataccatca actatcgcct gtgggtcagc gaaccaccat tgccggactc
    13681 agtgattgac gtcggtttgc gtacagaacc taaccttgaa ctgctgaccg aaatgaaacc
    13741 gtcgtttatg gtctggtcgg caggatatgg cccttcatca gaaatgctgg cacgtattgc
    13801 gccgggtcgc ggatttaact tcagtgacgg caaacatccg ttggcgatgg cgcgtaaatc
    13861 gctgacggaa atggcagatt tacttaacct gcaaagcgca gcggaaacgc atttagcgca
    13921 atacgaagac tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg cacgcccgtt
    13981 actgctgacg acgcttatcg atccgcgcca tatgctggtc ttcggtccaa acagcttgtt
    14041 ccaggaaatt ctcgatgagt acggcatccc aaatgcctgg caaggggaaa ccaacttctg
    14101 gggcagtacc gccgtcagta tcgatcgtct ggcagcgtat aaagacgttg atgtgctctg
    14161 ttttgatcac gacaacagca aagacatgga tgcgctaatg gcaaccccgc tgtggcaggc
    14221 catgccgttt gtccgtacag ggcgctttca gcgcgtaccc gcagtctggt tttatggtgc
    14281 aacgctttcg gcaatgcact ttgtgcgtgt tctggataac gccatcggag gtaaagcgtg
    14341 agtaaacgaa ttgcgctttt cccggcgtta ttgctggcgc tgttagtgat tgtcgctacg
    14401 gcgctcacct ggatgaactt ctcacaggcg ctgccgcgca gtcagtgggc gcaggccgcc
    14461 tggtcgccgg atattgacgt catcgagcag atgatttttc actacagctt gttgccgcgt
    14521 ctggcgattt cgctgctggt gggcgcgggc ctggggctgg tgggcgtgct gtttcagcaa
    14581 gtactgcgta acccgctggc ggaaccgacg acgttgggcg ttgcaacagg cgcgcaactg
    14641 gggattaccg tcaccacgct ctgggcgatc cctagcgcga tggcgagcca gtttgctgcg
    14701 ctggcagggg cttgcgttgt tggcttaatt gtctttggcg tcgcgtgggg gaaacggctg
    14761 tcgccggtaa cgctgattct cgcggggttg gtagtgagcc tttattgcgg cgcaatcaat
    14821 cagttactgg ttatcttcca tcatgaccag ttgcaaagca tgtttctgtg gagcactgga
    14881 acgctgacgc aaaccgactg gggcggtgtt gagcgtttat ggccgcagtt gctgggcggt
    14941 gtgatgctga cgttgctgct acttcgtccg ttaaccctga tggggcttga tgatggcgtg
    15001 gcgcgcaatc tcgggctggc cttgtcgctt gcgcgtctgg cggcgctgtc gctggcgatt
    15061 gtcatcagtg cgctgctggt gaacgctgtg gggattatcg gctttatcgg gttgttcgcg
    15121 ccgctgctgg caaaaatgct gggggcgcgg cgtctgctgc cacgactgat gctggcgtcg
    15181 ttgattggtg cgctgatcct ctggctttcc gatcaaatca tcctctggct gactcgcgtg
    15241 tggatggaag tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct gctactgtgg
    15301 ctgctgccgc gtttacgcag cattagcgcg ccggatatga aggttaacga tcgtgtcgcg
    15361 actgaacgcc aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgtt gttgatggct
    15421 gtggtggtgg cgctgtcgtt tggtcgtgat gcgcacggct ggacgtgggc gagcggggcg
    15481 ttgctcgagg atttaatgcc ctggcgctgg ccgcgaatta tggcggcgct gtttgcgggc
    15541 gtcatgctgg cggtagcggg ctgtattatt cagcgactga ccggcaaccc gatggcaagc
    15601 ccggaagtgc tggggattag ctccggcgcg gcgtttggag tggtgttaat gctgtttctg
    15661 gtaccgggta atgcctttgg ctggctgtta cccgcaggca gtctcggggc ggcggtgacg
    15721 ctgttgatca ttatgatcgc cgccggacgc ggtggatttt ccccacaccg catgttactg
    15781 gcggggatgg cgttaagtac cgcgttcacc atgcttttga tgatgttgca ggcaagtggt
    15841 gacccgcgaa tggcgcaagt gctgacctgg atttccggtt cgacctacaa cgcgaccgat
    15901 gctcaggtct ggcgcaccgg aattgtgatg gtgattttgc tggcgattac tccgctgtgc
    15961 cgccgctggc tgaccatttt accgctgggt ggtgataccg cgcgggcggt agggatggcg
    16021 ctgacgccga cgcgaattgc gctgctgctg ttagcggctt gcctgacggc gaccgcgacg
    16081 atgactattg gaccgttgag ttttgttggt ttaatggcac cgcatattgc gcggatgatg
    16141 ggctttcgac ggacgatgcc acacatcgta atttcggcgc tggtgggtgg tttactgctg
    16201 gtgttcgctg actggtgtgg gcggatggtg ctgtttccat tccagatccc ggcggggctg
    16261 ctgtcgacct ttatcggtgc gccatatttt atctatttgc tgagaaagca gagccgttaa
    16321 tagaatgcct cgcccggtgg gcgaggcaaa attatcacaa cttcgcgaac acccgacgtg
    16381 cagcatcgat ggtgttattg atatcttcca tgctgtgcgc cacagacata aagcccgctt
    16441 caaacgctga cggtgccagg taaacacctt catccagcat catatggaag aaacgcttaa
    16501 aacgctcgac gtcacaggcc atcacatcct gatagcacgt cacggactcg gcgtcggtaa
    16561 agaagatacc gaacatacca ccgacgtggt taacgaccag cggaattccc gattcttctg
    16621 ccgcttccag cagaccttct gccagacgtg ttgtcagctc atccagcgtt tcgtgaatgc
    16681 ccggctgcgc gacttcattc agacaggcga aacccgctgc catcgcaatc gggttaccgg
    16741 aaagcgtacc cgcctgatag accggacctg tcggggccag cgcatccatt acatcacgac
    16801 gaccaccgaa tgcgcctacc ggcattccac cgccgatgat ttttccgagg caggttaaat
    16861 ctggcactac gccgaaataa tcctgtgcgc cagctagcgc tacgcggaaa ccggtcatca
    16921 cttcatcgat gatcagcaac gcgccaaatt cgtcgcacag cgcacgcaga cctggcagga
    16981 actctggcag cggcggaaca cagttcatat tgcctgccac cggctcgacg ataatacagg
    17041 caatctcttg cgggtattgc tcaaacgcgg cgcgtacaga ggccagatcg ttataagtac
    17101 aggttaaggt atgtttggcg aaatctgccg gaacgcccgg cgagtttggc tggcctaacg
    17161 tgagtgcgcc agaaccggct ttcaccagca ggcagtcagc gtgaccgtgg taacaacctt
    17221 caaatttaat gattttgtcg cgaccggtaa aaccacgggc caggcggatg gcgctcatcg
    17281 tcgcctcggt gccggagttc accatgcgca ccatatccat ggtcgggacc agttcggtca
    17341 ccagttgcgc cattttcact tccatttcgg tcggtgcacc aaagcttaaa ccacgctcgg
    17401 cggcttcaat cacggcattg cggatcgccg gatggttatg gcccagcacc atcggccccc
    17461 aggaaccgac ataatcgata taggctttgc catcaacatc gtacagataa gcgccgtccg
    17521 ctttttcgat aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca ggggagttca
    17581 caccgccagg gatcagctcg cgcgctgcgc tgtaaagatt ttcagactta ctcatggagg
    17641 gttcctgatt cgtagaaaaa gtgaatggct gctattctat gttattcata acaagttaaa
    17701 tactcgtcaa acatcaggct gcttgtactg gtgcaatcct gatttcttag agtataaaag
    17761 ttttgtgcat ttgaaacatt acgctttgca aaggattttc atggaacgtg cgagtaaaat
    17821 gccgtcatct tatttgtatg accaataagt gatcattgga tgaaaactga tactccctct
    17881 ttagaaacac cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca acttcttgag
    17941 cgcgataaaa ccccgttagc cattttgttt atggcggcag tcgtcggcac gcttgtcggg
    18001 ctggctgcgg ttgcttttga caaaggtgtc gcctggttgc agaaccaacg tatgggggcg
    18061 ctggtacata ctgctgataa ttatccgcta ctgttaaccg tcgcttttct ctgttcggcg
    18121 gtgctggcga tgttcggcta ctttttggtg cgcaaatacg cgcccgaagc agggggatcg
    18181 gggatcccgg aaattgaagg ggcgctggaa gatcaacgtc ctgttcgctg gtggcgtgta
    18241 ttgccggtga aattctttgg cgggctgggg acactcggcg gaggcatggt gctagggcgc
    18301 gaaggaccaa ccgtgcagat cggcggcaat atcggtcgca tggtgcttga tattttccgc
    18361 ctgaaaggtg atgaagctcg ccatacgctg ctggcaaccg gtgctgctgc ggggctggct
    18421 gcggccttta acgcgccgct ggcgggtatt ttgtttatta tcgaagagat gcgtccgcag
    18481 tttcgctata cgttaatttc gattaaagcg gtatttattg gcgtcattat gtcgaccatt
    18541 atgtaccgga tttttaatca tgaagttgcg ttgattgacg tcggtaaact ttctgatgcg
    18601 ccgcttaata cgctgtggct ttatctgatc ctcggtatta tttttggcat tttcggccct
    18661 atttttaata aatgggtgct ggggatgcag gatttgctgc accgtgtgca cggcggcaat
    18721 attaccaaat gggtactgat ggggggtgcg attggcggtc tgtgtggatt gctggggttt
    18781 gtggcaccag caacgtcggg cggcggtttt aacctgattc ctatcgctac cgcggggaat
    18841 ttcagcatgg gaatgctggt gtttatcttc gtcgctcggg tcattaccac cttactctgc
    18901 ttctcttccg gcgcgccggg cggtattttt gccccgatgc tggcgctggg tactgtgctg
    18961 ggaaccgctt tcggaatggt tgccgttgag ctgtttccgc aatatcacct ggaggcgggg
    19021 acgtttgcta ttgccggaat gggggcatta ctggcggcat ctattcgcgc gccgttaacg
    19081 gggatcattc tggttctgga gatgaccgat aactaccagc tcattttgcc aatgattatt
    19141 accggtcttg gcgcaacact attagcgcaa tttaccggcg ggaaacctct atactcggcg
    19201 attcttgcgc gcacgctggc aaaacaggaa gctgagcaac tggcgcgaag caaggccgca
    19261 tcagccagcg agaatacttg aacgaaatac cagggtatta gataatggcg attattattg
    19321 ggttagaatt tgcccgattg ccgatgtcgt ttggagcaaa atatgagtga tgacgtagca
    19381 ctgccgctgg agtttaccga cgcagcagcc aacaaagtta aaagcctgat cgctgacgaa
    19441 gataacccga atctgaaatt acgcgtgtat atcaccggtg gcggttgcag cggcttccag
    19501 tatggtttca cctttgatga tcaggtgaac gaaggcgata tgaccatcga aaaacagggc
    19561 gttggcctgg tggttgatcc gatgagcctg caatatctgg tcggcggttc cgttgactac
    19621 accgaaggac tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa aagcacctgc
    19681 ggttgcggtt cttcctttag catctaatct gttgttcatg tgccggataa actatccggc
    19741 acatcttctc tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc ttaagatgcc
    19801 aacgaatagc cgccagccga atcaatagcg tcacgaccat gcccatcata ctggctgttt
    19861 ccagtggtac ggaaaatgtg taataagccg tagcgtggac aataccgccg ataatacagg
    19921 cagttgcgta gatttctgta cgtaaaatca tggggatttc gcgggccagt acatcacgaa
    19981 tgatcccgcc gccaacgcca gtaatgacgc ccatacaaac cgcgattaac ggaccggctt
    20041 ccgcattaaa ggctttattc acgccaatgc cgacaaacac cgccagacca acggcgtcca
    20101 gcaccggcaa catccatttt ggtaagcgtc ttggctggcg caccagcacg atggtcagca
    20161 tgctggtgac catggcaacg accagatcgg tgggatcttt cacccaaaat accgggccgt
    20221 gatccagcgc catgtcgcga attgttccgt cgcctactgc ggtaaccacg cccagtacca
    20281 gaacaccaaa agggtccata cgcaattttc cggctaacaa aacgccggag atggcaaata
    20341 ccgctgtgcc gactatatcc agccaataga cgagcattgt tcaatcccca ctgagcacct
    20401 gaaaaggtca ggcactaatc tacctgtgaa agcgcattac agagctgttg tgctgcgagg
    20461 ataatacgtg ggcttgcacg ttcaaaccag tcactcgtga gaggaataac gggaattttg
    20521 agctgttcac cccagtattg tttgatttta ggaatttggt ccggtccgcc tgtaatgaca
    20581 atcgcctgtg gcgagcgtgc taacacctgt tcgcggctaa cttgcggcca gggaacccgg
    20641 ctgtctttaa agatgttttc tccgccacaa acttcgagta cctggttctg aatcgactct
    20701 tttccactgg taaatggcgg attaatgccg aattgcagaa aaacacgttt tttaggttta
    20761 tcagcatatt gcgctttcaa ttgcacgtac tgatccagca gggattgcgc ggcttgttcg
    20821 gccttgtctg gttgcggact ccagggggcc agttgacgta acgcattggc aatttgttca
    20881 atgcttgtcg catcgaccca catcactttt attcccagcg aagccagctg gtcaacctgc
    20941 cgctcggcat tacctccacg ccaggcaatc accagatcgg gtttcagcgc gacaatgcgt
    21001 tccagattca tcccctgcca ggtggaaacc tgctcaatct tttgcgcttg tggaggatag
    21061 tcggaatagc tgctgacccc aaccggcgtg atcccggcgg caaaggcaag ttcagtgttg
    21121 gcgggagaaa gcgtgatgac gcgcggcgcg gcgttgagcc acagtggcgc gagaaaagac
    21181 agggcgacca gcgccctgaa cagtgattta gccatgtgcc agtttctgca ccagtgactc
    21241 aaccatcagg ctggactgtt tagcggcaac agccaggaac tcatcgaagc taagatgaga
    21301 ctgttgatcg gccacgtcgg agatggcgcg tacgacaaca aacgggacgt tgaaattgtg
    21361 gcagacatgg gcgattgccg tcgcttccat ctctacagca atggcctgtg ggaagttgtg
    21421 gcggattttc gccagaccaa cagaaccgtt gatgaaagcg tcgccgctaa caatcaggcc
    21481 acgtacagcg ttaagattca gttcggcaat gcaggcctca gcggcagcga tcagtttatc
    21541 gtcagcttta aagcctgccg gacagcctgg taactgaccg tattcataac caaatgccgt
    21601 gacatccgcg tcgtgataac gcgcttcgtc cgagacaacg atatcgccca ctttcagcgt
    21661 tggtgccagg ccaccggcag aaccggtgtt aataatcaca tctggcttgc agtgttccaa
    21721 cagcagagtg gcacccagcg ccgcagcgac tttaccgatg cccgatttca gaagcgcaac
    21781 ctcggttcca ttcagttggc cggtatagat ttcgcaaccg ccgagactga tagtttgacg
    21841 gttttcgatt ttgtcacgca gcagcgtaac ttcttcttcc attgcaccaa tgatgccgat
    21901 tttcatagat ttactcgcga taagcccgat ttgaaggcat agtttaccat gcgcttacgg
    21961 ggaagcgtat ttctcacgcg ggagaggaca tggcacagat tgatttccga aaaaaaataa
    22021 actggcatcg tcgttaccgt tcaccgcagg gcgttaaaac cgaacatgag atcctgcgga
    22081 tcttcgagag cgatcgcggg cgtatcatca actctccggc aattcgtcgt ctgcaacaaa
    22141 agacccaggt ttttccactg gagcgcaatg ccgccgtgcg cacgcgtctt acccactcga
    22201 tggaagtcca gcaggtggga cgctacatcg ccaaagaaat tttaagccgt ctgaaagagc
    22261 ttaaattgct ggaagcatac ggcctggatg aactgaccgg cccctttgaa agcattgttg
    22321 agatgtcatg cctgatgcac gatatcggca atccgccgtt tggtcatttt ggcgaagcag
    22381 cgataaatga ctggtttcgc caacgtttgc acccggaaga tgccgaaagc cagcctctga
    22441 ctgacgatcg ctgcagcgtg gcggcactac gtttacggga cggggaagaa ccgcttaacg
    22501 agctgcggcg caagattcgt caggacttat gtcattttga ggggaatgca caaggcattc
    22561 gtctggtgca tacattgatg cggataaatc tcacttgggc acaggttggc ggtattttaa
    22621 aatatacccg tccggcgtgg tggcgtggcg aaacgcctga gacacatcac tatttaatga
    22681 aaaagccggg ttattatctt tctgaagaag cctatattgc ccggttgcgt aaagaactta
    22741 atttggcgct ttacagtcgt tttccattaa cgtggattat ggaagctgcc gacgacatct
    22801 cctattgtgt ggcagacctt gaagatgcgg tagagaaaag aatatttacc gttgagcagc
    22861 tttatcatca tttgcacgaa gcgtggggcc agcatgagaa aggttcgctc ttttcgctgg
    22921 tggttgaaaa tgcctgggaa aaatcacgct caaatagttt aagccgcagt acggaagatc
    22981 agttttttat gtatttacgg gtaaacaccc taaataaact ggtaccctac gcggcacaac
    23041 gatttattga taatctgcct gcgattttcg ccggaacgtt taatcatgca ttattggaag
    23101 atgccagcga atgcagcgat cttcttaagc tatataaaaa tgtcgctgta aaacatgtgt
    23161 ttagccatcc ggatgtcgag cagcttgaat tgcagggcta tcgggtcatt agcggattat
    23221 tagagattta tcgtccttta ttaagcctgt cgttatcaga ctttactgaa ctggtagaaa
    23281 aagaacgggt gaaacgtttc cctattgagt cgcgcttatt ccacaaactc tcgacgcgcc
    23341 atcggctggc ctatgtcgag gctgtcagta aattaccgtc agattctcct gagtttccgc
    23401 tatgggaata ttattaccgt tgccgcctgc tgcaggatta tatcagcggt atgaccgacc
    23461 tctatgcgtg ggatgaatac cgacgtctga tggccgtaga acaataacca ggcttttgta
    23521 aagacgaaca ataaattttt accttttgca gaaactttag ttcggaactt cgggctataa
    23581 aacgaatctg aagaacacag caattttgcg ttatctgtta atcgagactg aaatacatga
    23641 aaaaaaccac attagcactg agtgcactgg ctctgagttt aggtttggcg ttatctccgc
    23701 tctctgcaac ggcggctgag acttcttcag caacgacagc ccagcagatg ccaagccttg
    23761 caccgatgct cgaaaaggtg atgccttcag tggtcagcat taacgtagaa ggtagcacaa
    23821 ccgttaatac gccgcgtatg ccgcgtaatt tccagcagtt cttcggtgat gattctccgt
    23881 tctgccagga aggttctccg ttccagagct ctccgttctg ccaggggggc cagggcggta
    23941 atggcggcgg ccagcaacag aaattcatgg cgctgggttc cggcgtcatc attgatgccg
    24001 ataaaggcta tgtcgtcacc aacaaccacg ttgttgataa cgcgacggtg attaaagtcc
    24061 agctgagcga tggtcgtaag ttcgacgcga agatggttgg caaagatccg cgctctgata
    24121 tcgcgctgat ccaaatccag aacccgaaaa acctgaccgc aattaagatg gcggattctg
    24181 atgcactgcg cgtgggtgat tacaccgtag cgattggtaa cccgtttggt ctgggcgaga
    24241 cggtaacttc cgggattgtc tctgcgctgg ggcgtagcgg cctgaatgct gaaaactacg
    24301 aaaacttcat ccagaccgat gcagcgatca accgtggtaa ctccggtggt gcgctggtta
    24361 acctgaacgg cgaactgatc ggtatcaaca ccgcgatcct cgcaccggac ggcggcaaca
    24421 tcggtatcgg ttttgctatc ccgagtaaca tggtgaaaaa cctgacctcg cagatggtgg
    24481 aatacggcca ggtgaaacgc ggtgagctgg gtattatggg gactgagctg aattccgaac
    24541 tggcgaaagc gatgaaagtt gacgcccagc gcggtgcttt cgtaagccag gttctgccta
    24601 attcctccgc tgcaaaagcg ggcattaaag cgggtgatgt gatcacctca ctgaacggta
    24661 agccgatcag cagctttgcc gcactgcgtg ctcaggtggg tactatgccg gtgggcagca
    24721 aactgaccct gggcttactg cgcgacggta agcaggttaa cgtgaacctg gaactgcagc
    24781 agagcagcca gaatcaggtt gattccagct ccatcttcaa cggcattgaa ggcgctgaga
    24841 tgagcaacaa aggcaaagat cagggcgtgg tagtgaacaa cgtgaagacg ggcactccgg
    24901 ctgcgcagat cggcctgaag aaaggtgatg tgattattgg tgcgaaccag caggcagtga
    24961 aaaacatcgc tgaactgcgt aaagttctcg acagcaaacc gtctgtgctg gcactgaaca
    25021 ttcagcgcgg cgacagcacc atctacctgt taatgcagta atctccctca accccttcct
    25081 gaatacggga aggggttctc cttacaatct gtgaacttcg ccacaactcc atacatcttc
    25141 atcatccttt gggcatttgc acaatgccgt acgttacgta cttccttatg ctaagccgtg
    25201 cataacggag gacttatggc tggctggcat cttgatacca aaatggcgca ggatatcgtg
    25261 gcacgtacca tgcgcatcat cgataccaat atcaacgtaa tggatgcccg cgggcgaatt
    25321 atcggcagcg gcgatcgtga gcgtattggt gaattgcacg aaggtgcatt gctggtactt
    25381 tcacagggac gagtcgtcga tatcgatgac gcggtagcac gtcatctgca cggtgtgcgg
    25441 caggggatta atctaccgtt acggctggaa ggtgaaattg tcggcgtaat tggcctgaca
    25501 ggtgaaccag agaatctgcg taaatatggc gaactggtct gcatgacggc tgaaatgatg
    25561 ctggaacagt cgcggttgat gcacttgttg gcgcaggata gccgtttgcg ggaagaactg
    25621 gtgatgaacc tgattcaggc agaggagaat actcccgcac ttctgaatgg gcgcaacggc
    25681 tggggatcga tctcaatcaa ccgcgagtgg tggctattgt tgaggtcgac agcggtcagc
    25741 ttggcgtgga cagcgcaatg gcggagttac aacaactgca aaacgcgctg actacgcccg
    25801 agcgtaataa tctggtggcg attgtctcgc taaccgaaat ggtggtgttg aaaccggcgt
    25861 tgaactcttt tgggcgctgg gatgcagaag atcatcgtaa gcgagttgaa caactgatta
    25921 cccgcatgaa agagtacggc cagctgcgtt ttcgcgtttc actgggcaac tattttaccg
    25981 gtcctggcag tattgcccga tcctatcgta cggcgaaaac gacgatggtg gtgggtaaac
    26041 agcggatgcc agaaagtcgc tgctattttt atcaggatct gatgttacct gtgttactcg
    26101 acagtttgcg tggcgactgg caggccaacg aactggcgcg accgctggcg cggctgaaag
    26161 cgatggacaa taacggcttg ctgcgacgaa cgctggcggc gtggtttcgc cacaatgtgc
    26221 aaccgctggc aacgtcaaag gcgttgttta ttcatcgtaa taccctggag tattggctta
    26281 atcgtatatc ggaactgacc gggcttgatt tgggcaattt tgatgacagg ttgctgctgt
    26341 atgtggcgtt acaactggat gaagagcggt agattatgcg ttaaggttga ggcggcgctg
    26401 gctcattccc ctcagcccga aggggagagg ggaatgcccg agttaaccgt caattcggga
    26461 ttatttacgc gttagttttt ccagatcggc ttcaatctcg ctgatcttat tggttacgac
    26521 tgactccagg tgacgtaagt cgtcgaggat cttacgctta agatcaactt cgctgcggtc
    26581 acgctgacag atttgatcaa gctcatcaat gatataccgt agattcgggc tgatttcctg
    26641 gacttcttta taaccctgac ccacaccatc agcgacgacc gttttacgct gacgcggata
    26701 tttaaactta acgctcttgg cgaaaaactc gcctttgtct ttctggaaat agattttcag
    26761 aatatcgttg ttggcttcct gccggaggct gtaacgatca atttcttcag gattggtaat
    26821 acccagactt ttcagattgt cgtacatagc gttacctcaa aatgagtcag taaattgtgc
    26881 ttattttagc atttggcttg gcccgcccgg ctcgactgtg atcgcagaaa gcctttttct
    26941 tccagacttc tatcatggcg taaaaatcaa aaaattacct gctttattgt ggcgatattc
    27001 catgggatgg attagcacat ttatcttcgt tttgcgaatt acatgacagt ccgaaacaag
    27061 atgaaaaagt atttaaaaaa gggggaggaa gaatgagtag gacgaattat tctgagccag
    27121 aaagcatacg ggcagcatca tgctgcccgt atatgtactc ttaatcgatg gtacgcagca
    27181 gttcgttaat gccgactttg ccgcgagttt tcgcgtcaac tttcttaacg ataaccgcac
    27241 agtagaggct gtatttgcca tcttttgacg gcagattacc tgaaacaacc acagaccccg
    27301 ccggaacgcg accgtagtgg atttcgccgg tttcacggtc gtaaatacgg gtgctctgac
    27361 caatgtatac gcccatagag atgacggaac cttcttcgac aatcacccct tcaaccactt
    27421 cagagcgtgc gccaatgaag cagttatctt caataatggt cgggttagcc tgcagcggct
    27481 ccagcacgcc gccgatgccc acgccaccgg aaaggtggac gtttttacca atctgcgcac
    27541 aagaaccgac ggtcgcccag gtatcaacca tggtgccttc atcaacatat gcgccaatgt
    27601 taacgtaaga cggcatcagc acggtgttac gggcaataaa cgcaccctga cgtaccgccg
    27661 ctggtggcac aacgcggaag ccttctttct ggaaacgtgc ttcgtcgtag tcggcgaatt
    27721 tcatcggcac tttgtcgaag tagcggcttt ctgccccttc gatcacctga ttatcattaa
    27781 tacggaaaga gagcagcacc gcttttttca accactgatg cgtcacccac tgaccgtcaa
    27841 ttttttccgc tacacgcagt gcgccggaat ccagcagggc gatcacctga tttaccgctt
    27901 cgcgggtaac ggtgtctgca ttggctggcg tgatctcggc acggcgttca aaagcggttt
    27961 caataatgtt ctgtaactgc tgcattgtta aactcttttc atatcagtaa acacatcacc
    28021 ctttatcgtt tggattgagg gcctctgtca accgctgatg cacttcctgc tgcaactcgt
    28081 tattaagcgc acgccggtca gcggtggcaa ttatgaataa atcttctact cgctcgccaa
    28141 tggttgtaat tcgggcacca tgaagcgaaa ttcccagatc ggcaaaaatt ttcccgactc
    28201 gcgccagcag tccaggttgg tcgagggcga tcagttcgag gaacgatttg cggtcggtat
    28261 gggtcggcaa aaacgttact tcggtttcaa cagtaaaatg gcgtaatttg gcgggttggc
    28321 gacggggctg cggtggctgc cagctacttt gcgtcagcac ctgctccaga ccaaaccgaa
    28381 taacctcatg gcgatctgcg gacagcgggc tgccatcggg ttccagcacg ataaaggtat
    28441 ccatcgccat accgtcgcga gtggtgaaaa tttgtgcgtc gtgaacactt aaattgcggc
    28501 ggtctaattc ggcacagacg gcggcaaaca gataggggcg gtccgggctc cagataaaaa
    28561 tctcggtgcc tccacgcgta gcctgcgggc taagcaatac cagcggtttg cttaaatcat
    28621 gctgtaataa atggcgggca tgccaggcca gttgatttgg gctatggcgg acaaaatagt
    28681 tagcacgaca gcgtgaccaa atttggtgca gcgcctcttc gtcgatgtta tccatgcgca
    28741 gtagtgccag tgcctggagt tggtgatggc gaacccgttc gcgcatatcc ggcgtgtttt
    28801 gcattccgcg tcgtagctgc ttttcggtgg caaaatagag ttcgcgcaac agactttgct
    28861 tccagctatt ccacagcgtt tcgttggtgg cgcaaatgtc agccactgtc aggcatacca
    28921 gatagcgcag acgattttcc gtttgaactt cttcggcaaa ctgcttgatg acttcagggt
    28981 cctgaatatc gcggcgttgt gctgtcaccg acatcagcaa gtgctggcga accaaccagg
    29041 caaccagctg tgtttcgcgt gagttcagcc cgtggagttc tgcaaaatgc actacatcct
    29101 gagcaccgag aatggagtgg tcgccgccgc gtcctttggc gatatcgtga aacagcgcgg
    29161 cgatgaaaat cagctcagtt gatggcaggc gcggccagac gtccacacac aacggatggc
    29221 gctggcgcgt ttcttcactg gcaaaactct ccagtttcag catcacgcgg atagtatgtt
    29281 catccaccgt gtaggcgtgg aacagatcaa actgcatctg cccgacgata tgcgaccatt
    29341 gcggcatata cgcgccgagc acgctatggc gatgcattgg caatagcccg cgccgcaccg
    29401 ctccggggtg acgcagaatg ctcaaaaaca gttttcgtgc ttgcggaata ttacacagcg
    29461 gttgttgcag atggcgacgg gcatggcgta actggcgcag cgtggtggag taaatgccgg
    29521 tgatcgcact gttgcgcacc atggtgtaga acatacgcaa gatggcttcc ggctggcgca
    29581 taaatagtgt ttcatcacgc aggtcgatta gcgtaccgcg tagctgaaac tcatcgtcga
    29641 gtggacgtgg tttttcgtcg gcgggaaggg cgaggatggc ttcatcgaac agttgcagca
    29701 gcatctggtt gagttcactg acgcggcgtg taacgcggaa gtaatccttc atcatccgct
    29761 cgaccggttc gttgccttca ccactgtaat tcagacgctg agcgacgcta agttggcgat
    29821 cgaataacag acgattatcg taacggctga cgaccagatg cagggcaaag cgaatacgcc
    29881 acagtatatg cagacattcg tttaattccg cccgctccgc tgaggttaag aagccaaacc
    29941 cgaccatttc atccagcgat gttgcgccaa aatgacggcg ggccacccat tgcagagtgt
    30001 gaatatcgcg caagccgcca gggctacttt tgatgtctgg ttcaaggttg tagctggtgc
    30061 catggtaacg ctgatggcgc tggttctgtt cttcaacttt cgctgcgtag aacttgtcgg
    30121 aaggccagaa tccttcgcta aaaatatgtt tttgcagttc gaggaagagc gcaacatcgc
    30181 caattaataa gcgggattcg attaaattgg tggcgacggt taaatccgat aacccttcca
    30241 gcatgcactc ttcaagcgtg cgcacactat gaccgacttc cagctttaca tcccagagca
    30301 gcgttaacag ctcgcacact ttttgcgcct gatcgtccgg gagcttttta cggcttaaaa
    30361 tcagtaaatc gatgtcagaa agtggatgca gctcgccacg accgtagcca ccgacggcaa
    30421 ccaatgccag gtcggcaatc tggctgaatc ccgcttcaat ccataatcgt tgcaggagct
    30481 ggtctatgaa ctcggtacgt gcctcaatca actgttctgc agagatccca ttgtcaaagg
    30541 catcacccag ccaacgctgg aaagtatcga tatgggcttt tatcccacag acggttaatt
    30601 catcacgggg ccaggcgcat ggattttgcg gttgaccggg cagggtgggg agagcggtgt
    30661 ttgcgtactg ttctggaagg gtattcattg tgcgccaccc ataagattaa attatcgcat
    30721 taaaaaagcc ggcattcgcc ggcttcatct tattcgtcgt gcgagattat cgccgggatg
    30781 gtgtcatcct tgcgtagcgt caaaatttcg cagccgttat cagtcaccac aatagtatgc
    30841 tcatattgtg cagacaagct gcgatctttg gtttttaccg tccagccatc tttcatggtg
    30901 cggatctctt ttttacccgc gttgaccatt ggctcaatgg tgaacgtcat ccccggtttc
    30961 agcacgacgt tggtttcacg ggagtcatag tgcagcacct gcggttcttc atggaagccg
    31021 cgaccaatac cgtgtccgca atattcacga acgacggaga agccttctgc ttcgacaaat
    31081 ttctgaatcg ccgcaccgat ttcgcgcaga ttaatgcctg gttttaccat gcgtagcgcc
    31141 aggtacaggc tttcttgcgt gatgcggcac aggcgttcgc ccatgatggt cggcttaccg
    31201 acgataaaca ttttcgaggt atcgccgtgg aaaccatctt tgattacggt gacatcaatg
    31261 ttaacgatat cgccatcttt cagcagctta gcatcgtccg ggataccgtg gcacaccact
    31321 tcattaatag agatgcaaac ggatttcgga tagccgtgat agccgaggca ggcagaaacc
    31381 gcgtgttgtt cattaacaat gtaatcatta cagatgcgat ccagctcgcc ggtgctgacg
    31441 cccggtttaa catacggttc gatcatctcc agcacttcgg cagccagtcg gccagcgacg
    31501 cgcatttttt cgatatcttc tggggtcttg attgagatag ccattaattc tgtccatcag
    31561 cgtcggtgat accgacaata tatatgtaag tgccgtcaat ggtatcacac ccgggcaaat
    31621 tgagaatcat tctgaatttc gccaaacgtg ccactgaagg ttttctataa tagaaaattc
    31681 gacgtctgat gctgtacaca gcgccaacag ttattggtgt ccacgacgta tttgtggtat
    31741 aaagcgcgcc ggacttccga tccatttcgt atacacagac tggacggaag cgacaatctc
    31801 actttgtgta acaacacaca cgtatcggca catattccgg ggtgcccttt ggggtcggta
    31861 atatgggata cgtggaggca taaccccaac ttttatatag aggttttaat catggcaact
    31921 gtttccatgc gcgacatgct caaggctggt gttcacttcg gtcaccagac ccgttactgg
    31981 aacccgaaaa tgaagccgtt catcttcggt gcgcgtaaca aagttcacat catcaacctt
    32041 gagaaaactg taccgatgtt caacgaagct ctggctgaac tgaacaagat tgtttctcgc
    32101 aaaggtaaaa tccttttcgt tggtactaaa cgcgctgcaa gcgaagcggt gaaagacgct
    32161 gctctgagct gcgaccagtt cttcgtgaac catcgctggc tgggcggtat gctgactaac
    32221 tggaaaaccg ttcgtcagtc catcaaacgt ctgaaagacc tggaaactca gtctcaggac
    32281 ggtactttcg acaagctgac caagaaagaa gcgctgatgc gcactcgtga gctggagaaa
    32341 ctggaaaaca gcctgggcgg tatcaaagac atgggcggtc tgccggacgc tctgtttgta
    32401 atcgatgctg accacgaaca cattgctatc aaagaagcaa acaacttggg tattccggta
    32461 tttgctatcg ttgataccaa ctctgatccg gacggtgttg acttcgttat cccgggtaac
    32521 gacgacgcaa tccgtgctgt gaccctgtac ctgggcgctg ttgctgcaac cgtacgtgaa
    32581 ggccgttctc aggatctggc ttcccaggcg gaagaaagct tcgtagaagc tgagtaataa
    32641 ggcttgataa ctcccccaaa atagttcgag ttgcagaaag gcggcaagct cgagaattcc
    32701 cgggagctta catcagtaag tgaccgggat gagcgagcga agacaacgca tctgtggcgc
    32761 gaaatatgaa gggggagagc ccttatagac caggtagtac acgtttggtt agggggcctg
    32821 catatggccc cctttttcac ttttatatct gtgcggttta atgccgggca gatcacatct
    32881 ccgaggattt tagaatggct gaaattaccg catccctggt aaaagagctg cgtgagcgta
    32941 ctggcgcagg catgatggat tgcaaaaaag cactgactga agctaacggc gacatcgagc
    33001 tggcaatcga aaacatgcgt aagtccggtg ctattaaagc agcgaaaaaa gcaggcaacg
    33061 ttgctgctga cggcgtgatc aaaaccaaaa tcgacggcaa ctacggcatc attctggaag
    33121 ttaactgcca gactgacttc gttgcaaaag acgctggttt ccaggcgttc gcagacaaag
    33181 ttctggacgc agctgttgct ggcaaaatca ctgacgttga agttctgaaa gcacagttcg
    33241 aagaagaacg tgttgcgctg gtagcgaaaa ttggtgaaaa catcaacatt cgccgcgttg
    33301 ctgcgctgga aggcgacgtt ctgggttctt atcagcacgg tgcgcgtatc ggcgttctgg
    33361 ttgctgctaa aggcgctgac gaagagctgg ttaaacacat cgctatgcac gttgctgcaa
    33421 gcaagccaga attcatcaaa ccagaagacg tatccgctga agtggtagaa aaagaatacc
    33481 aggtacagct ggatatcgcg atgcagtctg gtaagccgaa agaaatcgca gagaaaatgg
    33541 ttgaaggccg catgaagaaa ttcaccggcg aagtttctct gaccggtcag ccgttcgtta
    33601 tggaaccaag caaaactgtt ggtcagctgc tgaaagagca taacgctgaa gtgactggct
    33661 tcatccgttt cgaagtgggt gaaggcatcg agaaagttga gactgacttt gcagcagaag
    33721 ttgctgcgat gtccaagcag tcttaattat caaaaaggag ccgcctgagg gcggcttctt
    33781 tttgtgccca tcttgtaaat tcagctaacc cttgtggggc tgcgctgaaa agcgacgtac
    33841 aatgtcgcta gtattaattc atttcaatcg ttgacagtct caggaaagaa acatggctac
    33901 caatgcaaaa cccgtctata aacgcattct gcttaagttg agtggcgaag ctctgcaggg
    33961 cactgaaggc ttcggtattg atgcaagcat actggatcgt atggctcagg aaatcaaaga
    34021 actggttgaa ctgggtattc aggttggtgt ggtgattggt gggggtaacc tgttccgtgg
    34081 cgctggtctg gcgaaagcgg gtatgaaccg cgttgtgggc gaccacatgg ggatgctggc
    34141 gaccgtaatg aacggcttgg caatgcgtga tgcactgcac cgcgcctatg tgaacgctcg
    34201 tctgatgtcc gctattccat tgaatggcgt gtgcgacagc tacagctggg cagaagctat
    34261 cagcctgttg cgcaacaacc gtgtggtgat cctctccgcc ggtacaggta acccgttctt
    34321 taccaccgac tcagcagctt gcctgcgtgg tatcgaaatt gaagccgatg tggtgctgaa
    34381 agcaaccaaa gttgacggcg tgtttaccgc tgatccggcg aaagatccaa tcgcaaccat
    34441 gtacgagcaa ctgacttaca gcgaagtgct ggaaaaagag ctgaaagtca tggacctggc
    34501 ggccttcacg ctggctcgtg accataaatt accgattcgt gttttcaata tgaacaaacc
    34561 gggtgcgctg cgccgtgtgg taatgggtga aaaagaaggg actttaatca cggaataatt
    34621 cccgtgatgg ataaataagg gtaagattcc gcgtaagtat cgcgggggcg taagtctggt
    34681 tataaggcgt tattgttgca ggcagtttgg tcacggccag cgcgcagcaa ccggagcgta
    34741 caaaagtacg tgaggatggc gagcactgct cggggccaaa atggcaaata aaatagccta
    34801 ataatccaga cgattacccg taatatgttt aatcagggct atacttagca cacttccact
    34861 gtgtgtgact gtctggtctg actgagacaa gttttcaagg attcgtaacg tgattagcga
    34921 tatcagaaaa gatgctgaag tacgcatgga caaatgcgta gaagcgttca aaacccaaat
    34981 cagcaaaata cgcacgggtc gtgcttctcc cagcctgctg gatggcattg tcgtggaata
    35041 ttacggcacg ccgacgccgc tgcgtcagct ggcaagcgta acggtagaag attcccgtac
    35101 tctgaaaatc aacgtgtttg atcgttcaat gtctccggcc gttgaaaaag cgattatggc
    35161 ctccgatctc ggcctgaacc cgaactctgc gggtagcgac atccgtgttc cgctgccgcc
    35221 gctgacggaa gaacgtcgta aagatctgac caaaatcgtt cgtggtgaag cagaacaagc
    35281 gcgtgttgca gtacgtaatg tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa
    35341 agataaagag atcagcgaag acgacgatcg ccgttctcag gacgatgtac agaaactgac
    35401 tgatgctgca atcaagaaaa ttgaagcggc gctggcagac aaagaagcag aactgatgca
    35461 gttctgattt cttgaacgac aaaaacgccg ctcagtagat ccttgcggat cggctggcgg
    35521 cgttttgctt tttattctgt ctcaactctg gatgtttcat gaagcaactc accattctgg
    35581 gctcgaccgg ctcgattggt tgcagcacgc tggacgtggt gcgccataat cccgaacact
    35641 tccgcgtagt tgcgctggtg gcaggcaaaa atgtcactcg catggtagaa cagtgcctgg
    35701 aattctctcc ccgctatgcc gtaatggacg atgaagcgag tgcgaaactt cttaaaacga
    35761 tgctacagca acaggggagc cgcaccgaag tcttaagtgg gcaacaagcc gcttgcgata
    35821 tggcagcgct tgaggaggtt gatcaggtga tggcagccat tgttggtgct gctgggctgt
    35881 tacctacgct tgctgcgatc cgcgcgggta aaaccatttt gctggccaat aaagaatcac
    35941 tggttacctg cggacgtctg tttatggacg ccgtaaagca gagcaaagcg caattgttac
    36001 cggtcgatag cgaacataac gctatttttc agagtttacc gcaacctatc cagcataatc
    36061 tgggatacgc tgaccttgag caaaatggcg tggtgtccat tttacttacc gggtctggtg
    36121 gccctttccg tgagacgcca ttgcgcgatt tggcaacaat gacgcccgat caagcttgcc
    36181 gtcatccgaa ctggtcgatg ggacgtaaaa tttctgtcga ttcggctacc atgatgaaca
    36241 aaggtctgga atacattgaa gcgcgttggc tgtttaacgc cagcgccagc cagatggaag
    36301 tgctgattca cccgcagtca gtgattcact caatggtgcg ctatcaggac ggcagtgttc
    36361 tggcgcagct gggggaaccg gatatgcgta cgccaattgc ccacaccatg gcatggccga
    36421 atcgcgtgaa ctctggcgtg aagccgctcg atttttgcaa actaagtgcg ttgacatttg
    36481 ccgcaccgga ttatgagcgt tatccatgcc tgaaactggc gatggaggcg ttcgaacaag
    36541 gccaggcagc gacgacagca ttgaatgccg caaacgaaat caccgttgct gcttttcttg
    36601 cgcaacaaat ccgctttacg gatatcgctg cgttgaattt atccgtactg gaaaaaatgg
    36661 atatgcgcga accacaatgt gtggacgatg tgttatctgt tgatgcgaac gcgcgtgaag
    36721 tcgccagaaa agaggtgatg cgtctcgcaa gctgaggata atccggctac agagagtcgc
    36781 gctatttgtt agcgtagggc ttcagtgata tagtctgcgc catctgatcg taagtagttg
    36841 gctttataag gtcaggtatg ccgtggtttt acacggcttt tttttgtata ggcttcagta
    36901 ttcctgagta ccgtaaaccc tgtcagggaa taaaaaacgc gtgatgttgt ctgctactca
    36961 accacttagc gaaaaattgc cagcgcatgg ctgccgtcat gttgcgatca ttatggacgg
    37021 caatggtcgc tgggcaaaaa agcaagggaa gattcgtgcc tttgggcata aagccggggc
    37081 aaaatccgtc cgccgggctg tctcttttgc ggcaaacaac ggtattgagg cgttaacgct
    37141 gtatgccttt agtagtgaaa actggaaccg accagcgcag gaagtcagtg cgttaatgga
    37201 actgtttgtg tgggcgctcg atagcgaagt aaaaagtctg caccgacata acgtgcgtct
    37261 gcgtattatt ggcgatacca gtcgttttaa ctcgcgtttg caagaacgta ttcgtaaatc
    37321 tgaagcgcta acagccggaa ataccggtct gacgctgaat attgcggcga actacggtgg
    37381 acgttgggat atagtccagg gagtcaggca actggctgaa aaggtgcagc aaggaaacct
    37441 gcaaccagat cagatagatg aagagatgtt aaaccagcat gtctgtatgc atgaactggc
    37501 ccctgtagat ttagtaatta ggactggggg agagcatcgc attagtaact ttttgctttg
    37561 gcaaattgcc tatgccgaac tttactttac agatgttctc tggcccgatt tcgatgaaca
    37621 agactttgaa ggggcgttaa atgcctttgc taatcgagag cgtcgtttcg gcggcaccga
    37681 gcccggtgat gaaacagcct gatgggggtc gcttttgctg aagtatcgcc tgatatctgc
    37741 ttttgtgtta atacccgtcg tcatcgcggc gttgtttctg ttgccgccgg tggggttcgc
    37801 cattgtaacg ctggtggtct gcatgctggc agcgtgggaa tggggacaac ttagcggttt
    37861 taccactcgt tcgcagcgag tatggttggc ggtgttatgc gggttattgt tggcgctgat
    37921 gctttttctg ttgccggaat atcaccgaaa tattcatcaa ccgctggttg aaatctcact
    37981 ttgggcttcg ttgggttggt ggattgtcgc gctattgctg gtgctgtttt acccaggttc
    38041 cgcagcaatc tggcgtaact ctaaaacatt gcgccttatt tttggtgtgc taaccattgt
    38101 tcccttcttc tggggcatgc tggcgttacg ggcctggcac tatgacgaga atcattacag
    38161 tggcgcaata tggctgctct atgtcatgat cctggtatgg ggcgctgact ccggcgcata
    38221 tatgtttggc aaattgtttg gtaaacataa gctggcaccg aaggtttctc cgggtaaaac
    38281 ctggcaaggc tttataggtg gactcgctac tgcagcggta atctcatggg gttatggcat
    38341 gtgggcgaat ctcgacgtcg ctcccgtcac cttactcatt tgctctattg tcgcagcgtt
    38401 agcctcagtg ctcggcgatc tgaccgagag tatgtttaag cgtgaagcag gaattaagga
    38461 cagcggtcat ttaattccag gacacggtgg tattttagat cgtattgata gcctgacggc
    38521 tgcggtaccg gtctttgctt gcttgttgtt actggtattc aggacgcttt aacggaaggt
    38581 aatatgctga gttttctctg ggatttggct tcgttcatcg ttgcactggg tgtacttatc
    38641 accgtgcatg aatttggtca tttctgggtt gcccggcgtt gtggtgttcg cgttgagcgt
    38701 ttctcaatag ggtttggtaa ggcgctctgg cggcgaactg ataagctcgg caccgaatat
    38761 gttatcgccc tgatcccgtt gggcggttat gtcaaaatgc tggatgagcg cgcagaaccg
    38821 gtcgttccgg aactccgcca ccatgccttc aataataaat ctgtcggcca acgagcggcg
    38881 attattgccg caggtccggt tgcaaacttc atttttgcta tctttgccta ctggctggtt
    38941 tttattattg gtgtgcctgg cgtacgtccg gtggttggcg aaatagcagc caattcgata
    39001 gctgcggaag cacaaattgc accaggtacg gaactaaaag ccgtagatgg tatcgaaacg
    39061 cctgattggg atgccgtgcg tttgcagttg gtcgataaaa ttggcgatga aagcaccacc
    39121 attacagtag cgccatttgg cagcgaccaa cggcgggatg taaagctcga tttacgtcac
    39181 tgggcgtttg agcctgataa agaagatccg gtatcttcgc tggggattcg tcctcgtggg
    39241 ccgcaaattg aacctgtact ggaaaatgtg cagccaaact cggcggcaag caaggcaggt
    39301 ttgcaagcag gcgacaggat cgttaaagtc gatggtcagc ccttaacgca gtgggtgacc
    39361 tttgtgatgt ttgtccggga taacccgggt aaatccttag cgttagaaat cgaaaggcag
    39421 gggagtccct tgtctttgac attaatcccg gagagtaaac cgggtaatgg taaagcgatt
    39481 ggttttgtcg gtattgagcc gaaagtcatt cctttgccag atgagtataa agttgtacgc
    39541 cagtatgggc cgttcaacgc catcgtcgaa gccacggaca aaacgtggca gctgatgaag
    39601 ctgacggtca gtatgctggg aaaattgatc accggtgatg tgaaactgaa caacctcagt
    39661 gggccgatct ctatcgccaa gggggctggg atgacagcgg aactcggggt agtttattac
    39721 ctgccgtttc ttgcgcttat tagcgtgaac ttagggataa ttaacctgtt tccgttgccc
    39781 gtacttgacg gggggcatct gctgttcctt gcgatcgaaa agatcaaggg cggaccggta
    39841 tccgagcggg ttcaagactt ttgttatcgc attggctcga ttctgctggt gctgttaatg
    39901 gggcttgcac ttttcaatga tttctctcgg ttatgagagt tagttaggaa gaacgcataa
    39961 taacgatggc gatgaaaaag ttgctcatag cgtcgctgct gtttagcagc gccaccgtat
    40021 acggtgctga agggttcgta gtgaaagata ttcatttcga aggccttcag cgtgtcgccg
    40081 ttggtgcggc cctcctcagt atgccggtgc gtacaggcga cacggttaat gatgaagata
    40141 tcagtaatac cattcgcgct ctgtttgcta ccggcaactt tgaggatgtt cgcgtccttc
    40201 gtgatggtga tacccttctg gttcaggtaa aagaacgtcc gaccattgcc agcattactt
    40261 tctccggtaa caaatcggtg aaagatgaca tgctgaagca aaacctcgag gcttctggtg
    40321 tgcgtgtggg cgaatccctc gatcgcacca ccattgccga tatcgagaaa ggtctggaag
    40381 acttctacta cagcgtcggt aaatatagcg ccagcgttaa agctgtcgtg accccgctgc
    40441 cgcgcaaccg tgttgaccta aaactggtgt tccaggaagg tgtgtcagct gaaatccagc
    40501 aaattaacat tgttggtaac catgctttca ccaccgacga actgatctct catttccaac
    40561 tgcgtgacga agtgccgtgg tggaatgtgg taggcgatcg taaataccag aaacagaaac
    40621 tggcgggcga ccttgaaacc ctgcgcagct actatctgga ccgcggttat gcccgtttca
    40681 acatcgactc tacccaggtc agtctgacgc cagataaaaa aggtatttac gtcacggtga
    40741 acatcaccga aggcgatcag tacaagcttt ctggcgttga agtgagcggc aaccttgccg
    40801 ggcactccgc tgaaattgag cagctgacta agatcgagcc gggcgagctg tataacggca
    40861 ccaaagtgac caaaatggaa gatgacatta aaaagcttct cggtcgctat ggttatgcct
    40921 atccgcgcgt acagtcgatg cctgaaatta acgatgccga caaaaccgtt aaattacgcg
    40981 tgaacgttga tgcgggtaac cgtttctacg tgcgtaagat ccgttttgaa ggtaacgata
    41041 cctcgaaaga tgccgtcctg cgtcgcgaaa tgcgtcagat ggaaggtgca tggttgggga
    41101 gcgatctggt cgatcagggt aaggagcgtc tgaatcgtct gggcttcttt gaaactgtcg
    41161 ataccgatac ccaacgtgtt ccgggtagcc cggaccaggt tgatgtagtc tacaaggtga
    41221 aagaacgtaa cactggtagc ttcaacttcg ggattggtta cggtactgaa agtggcgtga
    41281 gcttccaggc tggcgtgcaa caggataact ggttaggtac aggttatgct gttggtatca
    41341 acgggaccaa aaacgattac cagacctatg ctgaactgtc ggtaaccaac ccgtacttca
    41401 ccgtagatgg cgtaagcctc ggtggtcgtc tcttctataa tgacttccag gcagatgacg
    41461 ccgacctgtc cgactatacc aacaagagtt atggtacaga cgtgacgttg ggcttcccga
    41521 ttaacgaata taactcgctg cgtgcaggtc tgggttatgt acataactcc ctgtccaaca
    41581 tgcagcctca ggttgcgatg tggcgttatc tgtactctat gggtgaacat ccgagcacct
    41641 ctgatcagga taacagcttc aaaacggacg acttcacgtt caactatggt tggacctata
    41701 acaagcttga ccgtggttac ttcccgacag atggttcacg tgtcaacctg accggtaaag
    41761 tgaccattcc tggatcggat aacgaatact acaaagtgac gttagacacg gcgacttatg
    41821 tgccgatcga tgacgatcac aaatgggttg ttctgggtcg tacccgctgg ggttatggtg
    41881 atggtttagg cggcaaagag atgccgttct acgagaactt ctatgccggt ggttccagca
    41941 ccgtgcgtgg cttccagtcc aataccattg gtccgaaagc agtttacttc ccgcatcagg
    42001 ccagtaatta tgatccggac tatgattacg aatgtgcgac tcaggacggc gcgaaagacc
    42061 tgtgtaaatc ggatgatgct gtaggcggta acgccatggc ggttgccagc ctcgagttca
    42121 tcaccccgac gccgtttatt agcgataagt atgctaactc ggttcgtact tcattcttct
    42181 gggatatggg taccgtttgg gatacaaact gggattccag ccaatattct ggatatccgg
    42241 actatagtga tccaagcaat atccgtatgt ctgcgggtat cgcattacaa tggatgtccc
    42301 cattggggcc gttggtgttc tcctacgccc agccgttcaa aaagtacgat ggagacaagg
    42361 cagaacagtt ccagtttaac atcggtaaaa cctggtaagt gttctccaca aaggaatgta
    42421 gtggtagtgt agcgatgact ttaggcgatc aatataagat cgccgggcca cgcaaagaac
    42481 tgcaccctcc ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg gttattagct
    42541 gcaggtcttg gtttagcact ggcaacttct gctcaggcgg ctgacaaaat tgcaatcgtc
    42601 aatatgggca gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa cacgctggaa
    42661 aatgagttca aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa
    42721 atgaaaaaac tgcagtccat gaaagcgggc agcgatcgca ccaaactgga aaaagacgtg
    42781 atggctcagc gccagacttt tgctcagaaa gcgcaggctt ttgagcagga tcgcgcacgt
    42841 cgttccaacg aagaacgcgg caaactggtt actcgtatcc agactgctgt gaaatccgtt
    42901 gccaacagcc aggatatcga tctggttgtt gatgcaaacg ccgttgctta caacagcagc
    42961 gatgtaaaag acatcactgc cgacgtactg aaacaggtta aataagtaat gccttcaatt
    43021 cgactggctg atttagcgca gcagttggat gcagaactac acggtgatgg cgatatcgtc
    43081 atcaccggcg ttgcgtccat gcaatctgca caaacaggtc acattacgtt catggttaac
    43141 ccaaaatacc gtgagcattt aggcttgtgc caggcgtccg cggttgtcat gacccaggac
    43201 gatcttcctt tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct gacttacgcg
    43261 cgcatggcgc aaattttaga taccacgccg cagcccgcgc agaacattgc acccagtgcg
    43321 gtgatcgacg cgacggcgaa gctgggtaac aacgtatcga ttggcactaa cgcggtgatt
    43381 gagtccggcg ttgaactggg cgataacgtg attatcggtg ccggttgctt cgtaggtaaa
    43441 aacagcaaaa tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta ccatgagatc
    43501 cagatcggtc agaattgcct gatccagtcc ggaacagtgg taggcgcaga cggctttggt
    43561 tatgccaacg atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt aattattggc
    43621 gatcgcgtgg agatcggtgc ctgcacaacc atcgatcgcg gcgcgctgga tgacactgtt
    43681 attggcaatg gcgtgatcat tgataaccag tgccagattg cacataacgt cgtgattggc
    43741 gacaatacgg cggttgccgg tggcgtcatt atggcgggca gcctgaaaat tggtcgttac
    43801 tgcatgatcg gcggagccag cgtaatcaac gggcatatgg aaatatgcga caaagtgacg
    43861 gttacgggca tgggtatggt gatgcgtccc atcactgaac caggcgtcta ttcctcaggc
    43921 attccgctgc aacccaacaa agtctggcgc aaaaccgctg cactggtgat gaacattgat
    43981 gacatgagca agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc
    44041 atcttttgtt cgccaaactt tacggcctgt ctcattctta cgattgcggc aggccgtgtt
    44101 attattgtcg tttcttatat tttgacagga agagtatctt gactactaac actcatactc
    44161 tgcagattga agagatttta gaacttctgc cgcaccgttt cccgttctta ctggtggatc
    44221 gcgtgctgga ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg
    44281 agccattctt ccagggccat ttccctggaa aaccgatttt cccgggtgtg ctgattctgg
    44341 aagcaatggc acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg
    44401 gtgagctgta ctacttcgct ggtattgacg aagcgcgctt caagcgcccg gtcgtgcctg
    44461 gcgatcaaat gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta
    44521 aaggggttgc tctggtcgat ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta
    44581 gccgggaggc ctgatacgtg attgataaat ccgcctttgt gcatccaacc gccattgtgg
    44641 aagagggcgc gtcaattggc gcgaacgcac acattggtcc tttttgtatc gttggacccc
    44701 atgtcgaaat tggtgagggt accgtactga aatctcacgt tgtcgtgaat ggtcatacta
    44761 aaattggccg cgataatgag atttatcagt ttgcctccat cggcgaagtt aaccaggatt
    44821 tgaaatatgc tggcgaaccg acccgtgtgg aaatcggcga tcgtaaccgc attcgcgaaa
    44881 gcgtcaccat tcatcgtggc acagtccagg gcggtggatt gacgaaggtg ggcagcgaca
    44941 acttactgat gatcaacgcg cacattgcgc acgattgtac ggtaggtaac cgctgtattc
    45001 tcgccaacaa cgcaacgctg gcgggtcacg tatcggttga cgacttcgcg atcatcggcg
    45061 gcatgaccgc agtccatcag ttctgcatca ttggtgcgca tgtgatggtt ggcggctgct
    45121 ccggtgtggc gcaggacgtc cctccttatg tcattgcgca gggtaaccac gcaacgccgt
    45181 tcggtgtcaa tatcgaaggg ctgaagcgcc gcggattcag ccgtgaggcg attaccgcta
    45241 tccgcaatgc gtataagcta atttatcgta gcggtaaaac gctcgatgaa gtgaaaccgg
    45301 aaattgctga actggcggaa acatatccgg aagtgaaagc ctttaccgat ttctttgcac
    45361 gctcaacgcg cggtctgatt cgttaatgac tgaccagcgt ccattaacga ttgccctggt
    45421 cgccggagaa acctccggcg atatcctggg ggccggttta atccgcgctc tgaaagaacg
    45481 tgtgcccaac gcccgctttg ttggtgttgc cgggccacga atgcaggctg aaggctgcga
    45541 agcctggtac gaaatggaag aactggcggt gatgggcatt gttgaagtgc tcggtcgtct
    45601 gcgtcgctta ctgcatattc gtgccgatct gacaaagcgt tttggcgaac tgaagccaga
    45661 tgtttttgtt ggtattgatg cgcccgactt caatattact cttgaaggta acctcaaaaa
    45721 gcagggtatc aaaaccattc attacgtcag tccatccgtc tgggcgtggc gacagaaacg
    45781 cgttttcaaa ataggcagag ccaccgatct ggtgctcgca tttctgcctt tcgaaaaagc
    45841 gttttatgac aaatacaatg taccgtgccg ctttatcggt cataccatgg ctgatgccat
    45901 gccgttagat ccagataaaa atggtgcccg tgatgtgctg gggatccctt acgatgccca
    45961 ctgtctggca ttgttgccgg gcagccgtgg tgcagaagtc gaaatgctta gtgccgattt
    46021 cttgaaaact gcccagcttt tgcgccagac atatccggat ctcgaaatcg tggtgccgct
    46081 ggtgaatgcc aaacgccgcg agcagtttga acgcatcaaa gctgaagtcg cgccagacct
    46141 gtcggttcat ttgctggatg gaatgggccg tgaggcgatg gtcgccagcg atgcggcact
    46201 actggcgtcg gggacggcag ccctggagtg tatgctggcg aaatgcccga tggtggtggg
    46261 atatcgcatg aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa ctgattatgt
    46321 ctcgctgcca aatctgctgg cgggcagaga gttagtcaaa gagttattgc aggaagagtg
    46381 tgagccgcaa aaactggctg cggcgctgtt accgctgttg gcgaacggga aaaccagcca
    46441 cgcgatgcac gataccttcc gtgaactgca tcagcagatc cgctgcaatg ccgatgagca
    46501 ggcggcacaa gccgttctgg agttagcaca atgatcgaat ttgtttatcc gcacacgcag
    46561 ctggttgcgg gtgtggatga agtcggacgc gggccgttag ttggcgcggt cgtcaccgct
    46621 gcggtgatcc ttgacccggc gcgcccgatt gccgggctga atgattccaa aaagctgagc
    46681 gaaaaacgcc gtctggcgct ctatgaagag atcaaagaga aagcgttgag ctggagtctg
    46741 ggccgcgcgg aaccccacga aatcgacgag ctgaacattc ttcatgcgac catgctggcg
    46801 atgcagcgtg ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat tgatggtaac
    46861 cgctgcccga aattaccgat gcctgcgatg gctgtggtga aaggggatag ccgcgtacca
    46921 gaaatcagtg ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat ggcggcgctg
    46981 gatattgttt tcccgcaata tggttttgcc caacataaag ggtatccaac cgcttttcat
    47041 ctggaaaaac tggctgaata tggcgcgact gaacatcatc gtcgcagctt tgggcctgtc
    47101 aaacgcgcac tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa ccggaatctg
    47161 aagatgtctg aaccacgttt cgtacacctg cgggtgcaca gcgactactc gatgatcgat
    47221 ggcttggcca aaaccgcacc gctggtaaaa aaggcggcgg cgttgggtat gccagcactg
    47281 gcgatcaccg atttcaccaa cctttgcggt ctggtgaagt tctacggagc gggacatggc
    47341 gcagggatta agcctatcgt cggggcagat tttaatgtcc agtgcgacct gctgggtgat
    47401 gagttaaccc acctgacggt actggcggcg aacaataccg gctatcagaa tctggcgttg
    47461 ctgatctcaa aagcgtatca gcgcgggtac ggtgccgccg ggccgatcat cgatcgcgac
    47521 tggcttatcg aattaaacga agggttgatc cttctttccg gcgggcgcat gggcgacgtc
    47581 ggacgcagtc ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc gttttatgaa
    47641 gaacacttcc cggatcgcta ttttctcgag ctgatccgca ccggcaggcc ggatgaagaa
    47701 agctatctgc acgcggcggt ggaactggcg gaagcgcgcg gtttgcccgt cgtggcgact
    47761 aacgatgtgc gctttatcga cagcagcgac tttgacgcac acgaaatccg cgtcgcgatc
    47821 cacgacggct ttaccctcga cgatcctaaa cgcccgcgta actattcgcc gcagcaatat
    47881 atgcgtagcg aagaggagat gtgcgagctg tttgccgaca tcccagaagc ccttgccaac
    47941 accgttgaga tcgccaaacg ctgtaacgta actgtgcgtc ttggtgaata cttcctgccg
    48001 cagttcccga ccggggacat gagcaccgaa gattatctgg tcaagcgtgc aaaagaaggc
    48061 ctggaagagc gtctgacatt tttatttcct gacgaggaag aacgtcttaa acgccgcccg
    48121 gaatatgacg aacgtctgga gactgaactt caggttatca accagatggg cttcccgggc
    48181 tacttcctca tcgttatgga atttatccag tggtcgaaag ataacggcgt accggtaggg
    48241 ccaggccgtg gctccggtgc gggttcactg gtagcctacg cgctgaaaat caccgacctc
    48301 gatccgctgg aatttgacct gctgttcgaa cgtttcctta acccggaacg tgtctccatg
    48361 cctgacttcg acgttgactt ctgtatggag aaacgcgatc aggttatcga acacgtggcg
    48421 gacatgtacg gtcgtgatgc ggtatcgcag attatcacct tcggtacgat ggcggcgaaa
    48481 gcggtgatcc gcgacgtagg ccgcgtgctg gggcatccgt acggctttgt cgaccgtatc
    48541 tcgaaactga tcccgcccga tccggggatg acgctggcga aagcgtttga agccgagccg
    48601 cagctgccgg aaatctacga agcggatgaa gaagttaagg cgctgatcga catggcgcgc
    48661 aaactggaag gggtcacccg taacgccggt aagcacgccg gtggggtggt tatcgcgccg
    48721 accaaaatta ccgattttgc gccgctttat tgcgatgaag agggcaaaca tccggtcacc
    48781 cagtttgata aaagcgacgt tgaatacgcc ggactggtga agttcgactt ccttggtttg
    48841 cgtacgctca ccatcattaa ctgggcgctg gagatgatca acaagcggcg ggcgaagaat
    48901 ggcgagccac cgctggatat cgccgcgatc ccgctggatg acaagaaaag cttcgacatg
    48961 ctgcaacgct cggaaaccac ggcggtattc cagcttgaat cgcgcggcat gaaggacctg
    49021 atcaagcgtc tgcaacctga ctgcttcgaa gatatgatcg cactggtggc actgttccgc
    49081 cctggtccgt tgcagtcagg gatggtggat aactttatcg accgtaaaca tggtcgtgaa
    49141 gagatctcct atccggacgt gcagtggcag catgaaagcc tgaaatcggt actggagcca
    49201 acctacggca ttatcttgta tcaggaacag gtcatgcaga ttgcccaggt gctttctggt
    49261 tataccctcg gtggcgcgga tatgctgcgc cgtgcgatgg gtaagaaaaa gccggaagag
    49321 atggctaagc agcgttctgt atttgctgaa ggtgcagaaa agaacggaat caacgccgaa
    49381 ctggcgatga aaatcttcga cctggtggag aaattcgcgg gttacggatt taacaaatcg
    49441 cactctgcgg cctatgcttt ggtgtcatat caaacgttat ggctgaaagc gcactatccg
    49501 gcggagttta tggcggcggt aatgaccgcc gatatggaca acaccgagaa ggtggtgggc
    49561 ctggtggatg agtgctggcg gatggggctg aaaattctgc caccagatat aaactccggt
    49621 ctttaccatt tccacgtcaa cgacgacggc gaaatcgtgt atggtattgg cgccatcaaa
    49681 ggggtaggtg aaggtccgat tgaggccatc atcgaagccc gtaataaagg cggctacttc
    49741 cgcgaactgt ttgatctctg cgcccgtacc gacaccaaaa agttaaaccg gcgggtgctg
    49801 gaaaaactga tcatgtccgg ggcgtttgac cgtcttgggc cacaccgcgc ggcgctgatg
    49861 aactcgctgg gtgatgcgtt aaaagcggca gatcaacacg cgaaagcgga agctatcggt
    49921 caggccgata tgttcggcgt gctggccgaa gagcaggaac aaattgaaca atcctacgcc
    49981 agctgccaac cgtggccgga gcaggtggta ttagatgggg aacgtgaaac gttaggcctg
    50041 tacctgaccg gacaccctat caaccagtat ttaaaagaga ttgagcgtta tgtcggaggc
    50101 gtaaggctga aagacatgca cccgacagaa cgtggtaaag tcatcacggc tgcggggctc
    50161 gttgttgccg cgcgggttat ggtcaccaag cgcggcaatc gtatcggtat ctgcacgctg
    50221 gatgaccgtt ccgggcggct ggaagtgatg ttgtttactg acgccctgga taaataccag
    50281 caattgctgg aaaaagaccg catacttatc gtcagcggac aggtcagctt tgatgacttc
    50341 agcggtgggc ttaaaatgac cgctcgcgaa gtgatggata ttgacgaagc ccgggaaaaa
    50401 tatgctcgcg ggcttgctat ctcgctgacg gacaggcaaa ttgatgacca gcttttaaac
    50461 cgactccgtc agtctctgga accccaccgc tctgggacaa ttccagtaca tctctactat
    50521 cagagggcgg atgcacgcgc gcggttgcgt tttggcgcga cgtggcgtgt ctctccgagc
    50581 gatcgtttat taaacgatct ccgtggcctc attggttcgg agcaggtgga actggagttt
    50641 gactaataca ggaatactat gagtctgaat ttccttgatt ttgaacagcc gattgcagag
    50701 ctggaagcga aaatcgattc tctgactgcg gttagccgtc aggatgagaa actggatatt
    50761 aacatcgatg aagaagtgca tcgtctgcgt gaaaaaagcg tagaactgac gcgtaaaatc
    50821 ttcgccgatc tcggtgcatg gcagattgcg caactggcac gccatccgca gcgtccttat
    50881 accctggatt acgttcgcct ggcatttgat gaatttgacg aactggctgg cgaccgtgcg
    50941 tatgcagacg ataaagctat cgtcggtggt atcgcccgtc tcgatggtcg tccggtgatg
    51001 atcattggtc atcaaaaagg tcgtgaaacc aaagaaaaaa ttcgccgtaa ctttggtatg
    51061 ccagcgccag aaggttaccg caaagcactg cgtctgatgc aaatggctga acgctttaag
    51121 atgcctgtca tcacctttat cgacaccccg ggggcttatc ctggcgtggg cgcagaagag
    51181 cgtggtcagt ctgaagccat tgcacgcaac ctgcgtgaaa tgtctcgcct tggcgtaccg
    51241 gtagtttgta cggttatcgg tgaaggtggt tctggcggtg cgctggcgat tggcgtgggc
    51301 gataaagtga atatgctgca atacagcacc tattccgtta tctcgccgga aggttgtgcg
    51361 tccattctgt ggaagagcgc cgacaaagcg ccgctggcgg ctgaagcgat gggtatcatc
    51421 gctccgcgtc tgaaagagct gaaactgatc gactccatca tcccggaacc gctgggcggc
    51481 gctcaccgta acccggaagc gatggcggca tcgttgaaag cgcaactgct ggcggatctg
    51541 gccgatctcg atgtgttaag cactgaagat ttaaaaaatc gtcgttatca gcgcctgatg
    51601 agctacggtt acgcgtaatt cgcaaaagtt ctgaaaaagg gtcacttcgg tggccctttt
    51661 ttatcgccac ggtttgagca ggctatgatt aaggaaggat tttccaggag gaacacatga
    51721 acatcattgc cattatggga ccgcatggcg tcttttataa agatgagccc atcaaagaac
    51781 tggagtcggc gctggtggcg caaggctttc agattatctg gccacaaaac agcgttgatt
    51841 tgctgaaatt tatcgaacat aaccctcgaa tttgcggcgt gatttttgac tgggatgagt
    51901 acagtctcga tttatgcagc gatatcaatc aacttaatga atatctcccg ctttatgcct
    51961 tcatcaacac ccactcgacg atggatgtca gcgtgcagga tatgcggatg gcgctctggt
    52021 tttttgaata tgcgctgggg caggcggaag atatcgccat tcgtatgcgt cagtacacca
    52081 acgaatatct tgataacatt acgccgccgt tcacgaaagc cttgtttacc tacgtcaaag
    52141 agcggaagta caccttttgt acgccggggc ata
//
LOCUS       DBKEKI010000012        51165 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.012, whole genome shotgun sequence.
ACCESSION   DBKEKI010000012 DBKEKI010000000
VERSION     DBKEKI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.012"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1037
                     /gene="pdeC"
                     /locus_tag="ACUQPX_000676"
     CDS             <1..1037
                     /gene="pdeC"
                     /locus_tag="ACUQPX_000676"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HHV9055468.1"
                     /translation="FSLSKEASAATFSPLIHLKDLTVQRNGYLYATVYSTKRPIAAIV
                     ATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCL
                     YYQPIIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFR
                     DLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDV
                     EKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHL
                     IAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQ
                     LPARELTRGQ"
     gene            complement(1040..1363)
                     /gene="soxS"
                     /locus_tag="ACUQPX_000677"
     CDS             complement(1040..1363)
                     /gene="soxS"
                     /locus_tag="ACUQPX_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HHV9055469.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1449..1913
                     /gene="soxR"
                     /locus_tag="ACUQPX_000678"
     CDS             1449..1913
                     /gene="soxR"
                     /locus_tag="ACUQPX_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HHV9055470.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2619..3968
                     /gene="ghxP"
                     /locus_tag="ACUQPX_000679"
                     /gene_synonym="yjcD"
     CDS             2619..3968
                     /gene="ghxP"
                     /locus_tag="ACUQPX_000679"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HHV9055471.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTVFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFSMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4119..5768
                     /locus_tag="ACUQPX_000680"
     CDS             4119..5768
                     /locus_tag="ACUQPX_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HHV9055472.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5922..7214)
                     /locus_tag="ACUQPX_000681"
     CDS             complement(5922..7214)
                     /locus_tag="ACUQPX_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HHV9055473.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMVEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7392..8531)
                     /locus_tag="ACUQPX_000682"
     CDS             complement(7392..8531)
                     /locus_tag="ACUQPX_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HHV9055474.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALSLGLGLM
                     FGTAGLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYK
                     DAAGHLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLY
                     ANVFKKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPI
                     ILLSMYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSI
                     SVAFLGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(8528..8842)
                     /locus_tag="ACUQPX_000683"
     CDS             complement(8528..8842)
                     /locus_tag="ACUQPX_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HHV9055475.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9042..11000)
                     /gene="acs"
                     /locus_tag="ACUQPX_000684"
     CDS             complement(9042..11000)
                     /gene="acs"
                     /locus_tag="ACUQPX_000684"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HHV9055476.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPSVVEKLLEEKQAIAMPS"
     gene            11392..12828
                     /gene="nrfA"
                     /locus_tag="ACUQPX_000685"
     CDS             11392..12828
                     /gene="nrfA"
                     /locus_tag="ACUQPX_000685"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HHV9055477.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            12873..13439
                     /gene="nrfB"
                     /locus_tag="ACUQPX_000686"
     CDS             12873..13439
                     /gene="nrfB"
                     /locus_tag="ACUQPX_000686"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HHV9055478.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            13436..14107
                     /gene="nrfC"
                     /locus_tag="ACUQPX_000687"
     CDS             13436..14107
                     /gene="nrfC"
                     /locus_tag="ACUQPX_000687"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HHV9055479.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14104..15060
                     /gene="nrfD"
                     /locus_tag="ACUQPX_000688"
     CDS             14104..15060
                     /gene="nrfD"
                     /locus_tag="ACUQPX_000688"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HHV9055480.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            15080..16798
                     /locus_tag="ACUQPX_000689"
     CDS             15080..16798
                     /locus_tag="ACUQPX_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HHV9055481.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            16791..17174
                     /gene="nrfF"
                     /locus_tag="ACUQPX_000690"
     CDS             16791..17174
                     /gene="nrfF"
                     /locus_tag="ACUQPX_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HHV9055482.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17171..17767
                     /gene="nrfG"
                     /locus_tag="ACUQPX_000691"
     CDS             17171..17767
                     /gene="nrfG"
                     /locus_tag="ACUQPX_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HHV9055483.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18109..19422
                     /gene="gltP"
                     /locus_tag="ACUQPX_000692"
     CDS             18109..19422
                     /gene="gltP"
                     /locus_tag="ACUQPX_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HHV9055484.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(19500..20189)
                     /gene="yjcO"
                     /locus_tag="ACUQPX_000693"
     CDS             complement(19500..20189)
                     /gene="yjcO"
                     /locus_tag="ACUQPX_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HHV9055485.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20283..22430)
                     /gene="fdhF"
                     /locus_tag="ACUQPX_000694"
                     /pseudo
     CDS             complement(20283..22430)
                     /gene="fdhF"
                     /locus_tag="ACUQPX_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(22628..24094)
                     /gene="mdtP"
                     /locus_tag="ACUQPX_000695"
     CDS             complement(22628..24094)
                     /gene="mdtP"
                     /locus_tag="ACUQPX_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HHV9055486.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSTNGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTHLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24091..26141)
                     /gene="mdtO"
                     /locus_tag="ACUQPX_000696"
                     /pseudo
     CDS             complement(24091..26141)
                     /gene="mdtO"
                     /locus_tag="ACUQPX_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26141..27163)
                     /gene="mdtN"
                     /locus_tag="ACUQPX_000697"
     CDS             complement(26141..27163)
                     /gene="mdtN"
                     /locus_tag="ACUQPX_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HHV9055487.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGRIIS
                     LKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEG
                     KVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIIVDKPDPEMFRIGASAV
                     ANLEPQ"
     gene            complement(27182..27457)
                     /locus_tag="ACUQPX_000698"
     CDS             complement(27182..27457)
                     /locus_tag="ACUQPX_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HHV9055488.1"
                     /translation="MPTVLSRMAMHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(27666..29651)
                     /gene="yjcS"
                     /locus_tag="ACUQPX_000699"
                     /pseudo
     CDS             complement(27666..29651)
                     /gene="yjcS"
                     /locus_tag="ACUQPX_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
     gene            29942..30649
                     /locus_tag="ACUQPX_000700"
     CDS             29942..30649
                     /locus_tag="ACUQPX_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHV9055489.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVSHTGQEAISRIEG
                     EPDIALVVLDMMLPDTNGLRVLQQIQKLRPALPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRWVGALAVNEEKNGGLSFNGWYLDTIRCQLHSPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
     gene            complement(30646..31647)
                     /locus_tag="ACUQPX_000701"
     CDS             complement(30646..31647)
                     /locus_tag="ACUQPX_000701"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HHV9055490.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLNMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(31644..32504)
                     /locus_tag="ACUQPX_000702"
     CDS             complement(31644..32504)
                     /locus_tag="ACUQPX_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HHV9055491.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            complement(32519..33202)
                     /locus_tag="ACUQPX_000703"
     CDS             complement(32519..33202)
                     /locus_tag="ACUQPX_000703"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HHV9055492.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGGNNFAAQGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            complement(33257..34198)
                     /locus_tag="ACUQPX_000704"
     CDS             complement(33257..34198)
                     /locus_tag="ACUQPX_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHV9055493.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            complement(34229..35203)
                     /locus_tag="ACUQPX_000705"
     CDS             complement(34229..35203)
                     /locus_tag="ACUQPX_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HHV9055494.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            complement(35200..36720)
                     /locus_tag="ACUQPX_000706"
     CDS             complement(35200..36720)
                     /locus_tag="ACUQPX_000706"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HHV9055495.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            36834..39098
                     /locus_tag="ACUQPX_000707"
     CDS             36834..39098
                     /locus_tag="ACUQPX_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHV9055496.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDNILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LADFNRELRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSGQRV
                     EQSYPDLRSGERLIMIFALLAVVITAFAGWYIYRNLGSNLTAISRAMSRLAHGEADVT
                     FPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEALLHS
                     QKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNTNSPDAPRIQRAQQAAERGALLTQRL
                     LAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLTIEIEAQSPAWSAWIDVSQLENA
                     IINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGSGMSQAVKSQV
                     FEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAAKNL
                     SPAAVEQTAVNGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAASAE
                     IDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLRKPF
                     TRAQLAQVLGQIEG"
     gene            39179..39811
                     /locus_tag="ACUQPX_000708"
     CDS             39179..39811
                     /locus_tag="ACUQPX_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HHV9055497.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRAAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGGCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            39880..40209
                     /gene="yjdP"
                     /locus_tag="ACUQPX_000709"
     CDS             39880..40209
                     /gene="yjdP"
                     /locus_tag="ACUQPX_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HHV9055498.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(40356..41114)
                     /gene="phnP"
                     /locus_tag="ACUQPX_000710"
     CDS             complement(40356..41114)
                     /gene="phnP"
                     /locus_tag="ACUQPX_000710"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HHV9055499.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHAQGLFPLRWGVGDPIPIYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLIENALPSGFEAGFDGMEIGVA"
     gene            complement(41116..41550)
                     /gene="phnO"
                     /locus_tag="ACUQPX_000711"
     CDS             complement(41116..41550)
                     /gene="phnO"
                     /locus_tag="ACUQPX_000711"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HHV9055500.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            complement(41537..42094)
                     /gene="phnN"
                     /locus_tag="ACUQPX_000712"
     CDS             complement(41537..42094)
                     /gene="phnN"
                     /locus_tag="ACUQPX_000712"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HHV9055501.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEISLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            complement(42094..43212)
                     /gene="phnM"
                     /locus_tag="ACUQPX_000713"
     CDS             complement(42094..43212)
                     /gene="phnM"
                     /locus_tag="ACUQPX_000713"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF011984.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HHV9055502.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLVSLMDHS
                     PGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAALCRARQI
                     ALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGSHSG
                     NVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTQNPAQALN
                     LQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            complement(43209..44054)
                     /gene="phnJ"
                     /locus_tag="ACUQPX_000714"
     CDS             complement(43209..44054)
                     /gene="phnJ"
                     /locus_tag="ACUQPX_000714"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HHV9055503.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(44047..45111)
                     /gene="phnI"
                     /locus_tag="ACUQPX_000715"
     CDS             complement(44047..45111)
                     /gene="phnI"
                     /locus_tag="ACUQPX_000715"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HHV9055504.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(45111..45695)
                     /gene="phnH"
                     /locus_tag="ACUQPX_000716"
     CDS             complement(45111..45695)
                     /gene="phnH"
                     /locus_tag="ACUQPX_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HHV9055505.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLKALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(45692..46144)
                     /gene="phnG"
                     /locus_tag="ACUQPX_000717"
     CDS             complement(45692..46144)
                     /gene="phnG"
                     /locus_tag="ACUQPX_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HHV9055506.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(46145..46870)
                     /gene="phnF"
                     /locus_tag="ACUQPX_000718"
     CDS             complement(46145..46870)
                     /gene="phnF"
                     /locus_tag="ACUQPX_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HHV9055507.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(46891..47670)
                     /gene="phnE"
                     /locus_tag="ACUQPX_000719"
     CDS             complement(46891..47670)
                     /gene="phnE"
                     /locus_tag="ACUQPX_000719"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HHV9055508.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPLAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(47776..48792)
                     /gene="phnD"
                     /locus_tag="ACUQPX_000720"
     CDS             complement(47776..48792)
                     /gene="phnD"
                     /locus_tag="ACUQPX_000720"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HHV9055509.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(48817..49605)
                     /gene="phnC"
                     /locus_tag="ACUQPX_000721"
     CDS             complement(48817..49605)
                     /gene="phnC"
                     /locus_tag="ACUQPX_000721"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HHV9055510.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALPGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(49738..50181)
                     /gene="yjdN"
                     /locus_tag="ACUQPX_000722"
     CDS             complement(49738..50181)
                     /gene="yjdN"
                     /locus_tag="ACUQPX_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HHV9055511.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAITHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(50439..50774)
                     /locus_tag="ACUQPX_000723"
     CDS             complement(50439..50774)
                     /locus_tag="ACUQPX_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HHV9055512.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT        11286 a        13457 c        14179 g        12243 t
ORIGIN      
        1 ttttctcgtt aagcaaagag gcctcggcag caacgttttc tccgctgatt catttgaagg
       61 atttaaccgt acaaagaaat ggctatttat atgcgacagt ttattcgaca aaacgcccaa
      121 ttgcagccat tgttgcgact tcatatcaac gtcttataac ccatttttat aatcatctta
      181 tttttgcgtt gcccgccggt attttgggga gtcttgttct gctattactc tggctacgta
      241 ttcgacaaaa ctatttatct cccaaacgta aattgcaacg cgccctcgaa aaacatcaac
      301 tttgtcttta ttaccagcca ataatcgata tcaaaacaga aaaatgtatc ggcgctgaag
      361 cgttgttacg ttggcctggt gagcaggggc aaataatgaa tccggcagag tttattccgc
      421 tggcagaaaa ggaggggatg atagaacaga taactgatta tgttattgat aatgtcttcc
      481 gcgatctggg cgcgtacctg gcaacacatg ccgatcgcta tgtttctatt aacctgtcgg
      541 cctccgattt tcatacgtca cggttgatag cgcgaatcaa tcagaaaaca gagcaatacg
      601 cggtgcgtcc acagcaaatt aaatttgaag tgactgagca tgcatttctt gatgtcgaaa
      661 aaatgacgcc gattattctg gctttccgcc aggctggtta cgaagtggca attgatgatt
      721 ttggtattgg ctactctaac ttgcataacc ttaaatcatt gaatgtcgat attttgaaaa
      781 tcgataaatc gtttgttgaa acgctgacca cccacaaaac cagtcatttg attgcggaac
      841 acatcatcga gctggcgcac agcctggggt taaaaacgat cgcagaaggc gtcgaaactg
      901 aggagcaggt taactggctg cgcaaacgcg gcgtgcgcta ttgccaggga tggttttttg
      961 cgaaggcgat gccgccgcag gtgtttatgc aatggatgga gcaattaccc gcgcgggagt
     1021 taacgcgcgg gcaataaaac tacaggcggt ggcgataatc gctgggagtg cgatcaaact
     1081 gccgacggaa aacgcgggag aaggtctgct gcgagacata acccaggtcc attgcgatat
     1141 caaaaatcgg acgctcggtg gtgcgcaact caacggcggc cagtaacagg cggcgttggc
     1201 gaatgtaatc gccaagcgtc tgatgcgtca ccgtgcggaa catccgttgc aagtaccact
     1261 ttgaatagcc tgattttttt gcgacgacat caatgttaag cggctggtca atatgcacgt
     1321 caatccatgc gataagatcc tgaataattt tctgatggga cataaatctg cctcttttca
     1381 gtgttcagtt cgttaattca tctgttgggg agtataattc ctcaagttaa cttgaggtaa
     1441 agcgatttat ggaaaagaaa ttaccccgca ttaaagcgct gctaacccct ggcgaagtgg
     1501 cgaaacgcag cggtgtggcg gtatcggcgc tgcatttcta tgaaagcaaa gggttgatta
     1561 ctagtatccg taacagcggc aatcagcggc gatataaacg tgatgtgttg cgatatgtcg
     1621 caattatcaa aatcgctcag cgtattggca ttccgctggc gaccattggt gaagcgtttg
     1681 gcgtgttgcc agaagggcat acgttaagtg cgaaagagtg gaaacagctt tcgtcccaat
     1741 ggcgagaaga gttggatcgg cgcattcata ccttagtggc gctgcgtgac gaactggacg
     1801 gatgtattgg ttgtggctgc ctttcgcgca gtgattgccc gttgcgtaac ccgggcgacc
     1861 gcttaggaga agaaggtacc ggcgcacgct tgctggaaga tgaacaaaac taaagcgcca
     1921 caaggacaag ctgactcatc gttaactatg gggccggggt gagggtaaat atttgcactg
     1981 gtgctgcctg ttcccctcac cctaaccctc tccccgaagg ggcgagggga ctgatcgagc
     2041 tcttttggaa ttatccgcaa acacaaaact aaagcgccac aagggcgctt tagtttgttt
     2101 tccggtcttt gtctttctct ctatcccgct ggaacacagg aggggttccc ccgacgtcaa
     2161 cacacctcat tcgagcacgt ggtggaggtt ccggttggtg ttgatgcttt aattgtatgt
     2221 caccgacgtt tcttcgccag tgtaaaagta tactttttag ccgcaatatt tttgtcatct
     2281 cagacgattt tttatcgcaa tccagaacgg tatacggctc gataacgctg caatcttgcg
     2341 catcgacgat aacgtttgcg catcaattgc ctggtttttc atcgtcaaga caataaaaga
     2401 gaaaaaagca gcaaacttcg gttgaaaaag ccgctatgat cgccggataa tcgtttgctt
     2461 ttttttacca cccgttttgt atgcgcggag ctaaacgttt gcttttttgc gacgcaccaa
     2521 attgccgcaa acctggagca ggaagataac gtttcgctgg caggggattg tccgccacgc
     2581 atcttgacga aaattaaact ctcaggggat gttttcttat gtctacgcca tcagcgcgta
     2641 ccggcggttc actcgacgcc tggtttaaaa tttcacaacg tggaagcact gtccgtcagg
     2701 aagtggttgc cgggttaaca acgtttctgg cgatggtcta ctcggtcatc gtcgttccag
     2761 gtatgttggg taaagcaggc ttcccgcctg cggcagtttt cgttgcaacc tgtctggttg
     2821 ccggactcgg ttctatcgtg atgggtctgt gggctaatct gccgttggcg attggttgcg
     2881 ccatctccct gacggtgttt accgcattca gcctggtgct ggggcaacat attagcgtac
     2941 ctgtcgcgct gggtgccgtg ttcctgatgg gtgtgctgtt tacggtaatt tctgccacgg
     3001 gtatccgtag ctggattttg cgcaacttgc cgcacggtgt ggcgcacggc acggggatcg
     3061 gtatcggtct gttcctgctg ctcattgccg ctaatggtgt cggtctggtg attaaaaacc
     3121 cgcttgatgg tctgcccgtt gcgctgggtg atttcgcgac cttcccggtg attatgtcac
     3181 tggtaggtct ggcggtgatc atcggcttgg aaaaactgaa agtccctggt ggcattctgc
     3241 tgaccattat cggtatctca attgtcggtt tgatcttcga tcctaacgtc catttctccg
     3301 gcgttttctc catgccttca ttgagcgatg aaaacggcaa ttcactgatt ggcagcctgg
     3361 acattatggg cgcgctgaat cctgtagtcc tgccaagcgt tctggcgctg gtgatgacgg
     3421 cagtatttga tgccaccgga actatccgcg ctgtcgccgg ccaggcgaac ctgctggata
     3481 aagatgggca gatcatcgac ggcgggaaag cactgaccac tgactccatg agcagcgttt
     3541 tctctggcct ggtgggtgca gctccggcag cggtatacat cgagtctgcg gcgggtacgg
     3601 cggcgggcgg taaaaccggg ttgacggcta tcaccgttgg cgtgctgttc ctgctgattc
     3661 tgttcctctc tccgctttct tacctcgttc cggggtatgc aacggctccg gcgctgatgt
     3721 acgttggcct gctgatgctg agcaacgtgg cgaaaatcga ctttgctgat tttgttgatg
     3781 cgatggcggg tctggttacg gcggtattca tcgtgctgac ctgtaacatc gtaacaggca
     3841 tcatgatcgg cttcgcgact ctggtgattg gtcgtctggt ttccggtgaa tggcgcaagt
     3901 tgaacatcgg tacggtcgtt atcgccgtgg cgctggtgac cttctatgcg ggtggctggg
     3961 ctatctaatc tttcatcgga ttctgaaaac gggtggcaat ggctgcccgt ttttattttc
     4021 tccgcacatt gtgtgagctt ttgcgatatt ctgaaaaaat gagaattcag gcataacgtc
     4081 tttctcagat cataaaaaac atcgcaaaca gggaacgcat ggaaatcttc ttcaccatac
     4141 tgataatgac cctcgtggtc tcgctgtccg gggtggtcac tcgtgtcatg ccctttcaga
     4201 tcccgcttcc gcttatgcaa atcgccatcg gtgcgctact ggcgtggccg acgtttggtt
     4261 tgcatgtgga gtttgatcct gaactctttt tagtcttgtt tatcccgccg ttgctgttcg
     4321 ctgatggctg gaaaacgccg acccgtgaat ttctcgaaca tggtcgagag attttcggcc
     4381 tcgcgctggc gctggtggtg gtcaccgtgg tcggcattgg cttccttatt tactgggtgg
     4441 tgccgggcat tccgctgatc cccgcctttg cgctggcggc ggtactttct ccgaccgatg
     4501 ctgtggcgct ctccgggatt gttggcgaag ggcgcatccc gaaaaaaatc atgggcattt
     4561 tgcagggcga agcgttgatg aatgacgctt ccggcctggt gtcgttgaag tttgccgtgg
     4621 cagtggcgat ggggacgatg atcttcactg tcggcggtgc gacggtcgaa tttatgaaag
     4681 tagccattgg cggtattctt gccggttttg tggtgagctg gctgtatggt cgctcgctgc
     4741 gattcctcag ccgctggggc ggtgatgaac ccgcgacgca gatcgttctg ctgttcttgc
     4801 tgccattcgc ttcttatctg attgccgaac atattggcgt ttcgggcatc ctcgctgcgg
     4861 ttgccgccgg gatgaccatc acccgctccg gtgtgatgcg ccgtgcgcca ctggcaatgc
     4921 gcctgcgcgc aaacagcacc tgggcgatgc tggaatttgt ctttaacggc atggtgttcc
     4981 tgctgttagg tctgcaactg ccgggtattc tggagacgtc gctgatggcg gcagaaatcg
     5041 accctaacgt cgaaatctgg atgctgttta ccgatattat tctgatatat gcggcgctga
     5101 tgctggtccg tttcggctgg ctgtggacga tgaaaaagtt cagcaaccgc ttcctgaaga
     5161 agaagccgat ggagtttggt tcgtggacca cacgagaaat cctgatcgcg tctttcgctg
     5221 gggtgcgtgg ggcgatcact ctggccggtg tgctctcaat cccgctgctc ttgccggatg
     5281 gtaacgtctt cccggcgcgc tatgagctgg tgttcctggc ggctggcgtc attctcttct
     5341 cgctgtttgt cggcgtggtg atgttgccta ttctgctaca acacattgaa gtcgcggacc
     5401 attcgcaaca attgaaagaa gaacgtattg cgcgagcggc aacggcagaa gtggcgattg
     5461 tggcgatcca gaaaatggag gagcgtctgg cggcggatac cgaagagaat atcgataacc
     5521 agttgcttac ggaggtcagt tctcgcgtca ttggtaacct gcgtcgtcgc gccgatggac
     5581 gtaacgacgt tgaaagttcc gtgcaggaag agaaccttga gcgtcgcttc cgtctggcgg
     5641 cattgcgttc tgaacgtgct gaactgtacc acctgcgcgc cacgcgggag atcagcaacg
     5701 aaacgctgca aaaattactg cacgatctcg atttgcttga agcgttgcta attgaggaaa
     5761 atcagtaagc cgaaagcgac gtaaccaggc ccgaggtaat ctgaaaaagg tgcaatcttt
     5821 tgcaccttta acaggttagc tcgggcgaac attcatgatt ctaaaacaaa taagttatct
     5881 ctcttgtaaa gtcaattcat tggctgttct tcgacttaac ctcaatctgc gtgttgcata
     5941 atatctgcag taatcttcat agcgactgca ttaatattat cctcagtttg tgacatgcca
     6001 gtatgagagg tacaatcttc ttcaaagaat ttctgtagtg ttaccagggt atcactatca
     6061 atggggatat tttcctcccc acggagaatg gaaatgccca ttccctggac gctagcctca
     6121 tcgaaattaa cccgatagag acgggtatta aacatacagg cttttgttaa aatagaacag
     6181 ttgaaatggc agttgcttaa attggcatta ttaaaattaa cattagccat attagagccg
     6241 gtacatataa ccatttcgag gaatgtattt gtcagccacg tacaaaagag tttgctatca
     6301 attaagttta tattctggag gattgctttg ttcagattga cgcgagacag atcagcatgt
     6361 tcaaagttaa ttaaatcgag tttagagaaa ctaaggtcgg ctttaaataa attaacatat
     6421 ggcgctatta ctttataaag atttacctca accatgtacg ccatataaaa atttgaatag
     6481 tcaaggaagg tatttttaat attggctcta ttcattgctg cattgtagaa ttgaacatgc
     6541 atagcggagc atgcatttag agttccgtcg gccattttta tctgagcgaa atttgagtca
     6601 tcgagaatac aattatcaaa aagtattttt tccattatgg cataataaaa attcgtttta
     6661 tacatattac agtcctggaa aatagcgttt tgtagtctgc aacctgcaaa attcgaattg
     6721 gataaatctt catctttaat cacgataccc ttcaggctaa ggtgtgaaaa atcgtaacca
     6781 ccatttacta tctgtggaat ttcgatagta ttattatgga cggcgacatt atttataaaa
     6841 tataccacct taatggatac atccttttct ttactaaggt aaactcttct cgtaattcta
     6901 tcgtcacatt tattatttgc atataacgct atatctataa aatatttatg tattttcctg
     6961 tccgtttctt tcgtgatttt tcctgatggt aaactatcat aaattctttt cattttctga
     7021 atcaagccta tcactttttc atgatcatct attttgaaaa gagagagggc tttattgtca
     7081 attaacgtgt ttttatgatg tttgctgtaa ttatctgatt ttgttttctt tgtatgtgat
     7141 gaacttgtga tagaatggtt atcgttaatc tggcaaagag ggaaaaacat attatttaaa
     7201 ccattatagc gcataaaata tccttataat taacaagaaa gaaaaggcat attctctgca
     7261 ttatcatttt ctacgactgt caaaaatcgc tcatttttta atgagtttat ttgtttaata
     7321 ttatgggaaa aggtgatgca tgttggagag gaagagtatt ccccggtcag gcgaccgggg
     7381 aaggggagag attaatgtgc gcggccttgc tcaacgccaa agccggtctg ggagcggata
     7441 aactgcgcgc ggaacagttc acgctcgcgc gccccttccg ctgagttatc ggttgccgag
     7501 aagaaccaga tgccgaggaa tgccacgctg atagagaaca gcgccgggta ttcatacggg
     7561 aagatggctt tttcgtgacc gaggatctgt acccaaatag tcgggccgag gatcatcagc
     7621 accactgcgg taatcagccc cagccagccg cccagcatcg cgccacgggt agtcagtttc
     7681 gaccagtaca tggaaagcag aatgatcggg aagttacagc tcgccgcgat ggcaaacgca
     7741 aggcctacca taaaggcgat gttctgattc tcaaacagca cgccgaggat aatcgcaatc
     7801 acgccgagga tcagtacggt gatttttgat acccgcagct cttcacgttc ggtcgcgcct
     7861 tttttgaaga cgttagcgta caagtcatgc gaaaccgccg atgcgcccgc cagcgtcaga
     7921 cccgcaacca ccgcgaggat agtggcgaaa gcaaccgctg aaataaaacc gaggaatagg
     7981 ttgccgccca ctgcattcgc caggtgaacg gccgccatgt tgttaccacc aatcagatgg
     8041 cccgccgcgt ctttatattc cggattcgca ccaacaagca tgatcgcgcc gaagccgata
     8101 ataaaggtca gaatatagaa gtagcccata aatccggtgg cgtagaacac gctcttacgt
     8161 gcttcgcggg catcgctgac tgtaaagaag cgcatcagaa tgtgcggcaa gcccgccgta
     8221 ccaaacatca gtcccagccc cagagagagc gcggagatcc ccaagaatga ggcggcggac
     8281 atatagtccc cggcaatcgc cagcccgttc tggaagccag tgatattgcc gcctgcggtg
     8341 tagtagtcgc tacgagaacg tacgcgtttt gatgcccagt aggtaatgcc gagcgtaaac
     8401 acgacgaaaa tcaggaacat aataatcgcc tgccagttcg ttggctggcg ctctacggcc
     8461 ccgctaatag catccgcggc gttagctgcg aaagggagtg tggcggcaag cgccgtcaga
     8521 actcttttca tgatgcttgt acctcatgca ggacttcatt attaagacgg tcgaattcgc
     8581 cgttcgcccg ccagatgtag ataccggtga gaacaaagga gatcacaatc actccaacac
     8641 caattggaat accgcgtgtg acgctggtgt tcggattcag cggcgtgccc agccagccgg
     8701 gcgcgaaggc gatcagtaaa ataaagccga tataaactgc cagcataata atcgacagga
     8761 tggtggcaaa ccgttgccgt ttttcgacta actccctgaa atgcgcattg tcttctatcc
     8821 gctgataaat cgtaccattc atcacagatt ctccagaggt aatgtaggga ttgttttaat
     8881 tcccgctccc ttatgggaga aggttaacgc tcgggtaacc cttgccgaat gtaggccgga
     8941 taaggcgttt acgccgcatc cggcaatcaa tgcctgatgc gacgctgtcg cgtcttatca
     9001 ggcctacaga ccgttaccga ttcgcatccg gcaattgtgg gttacgatgg catcgcgata
     9061 gcctgcttct cttcaagcag cttctcgacc acgctaggat cggcaagcgt cgaggtatcg
     9121 cccaggttgc tggtatcgcc cgccgcaatt ttgcgcagaa tacggcgcat aattttgccg
     9181 gagcgggttt taggcaggga gtcggtccag tgcagcacgt ctggcgtcgc cagcgggcca
     9241 atctctttac gcacccagtt gcggacttct gcgtacagtt ctggtgacgg ttcctccccg
     9301 tgattaagcg tgacataggc gtagatcgcc tgacctttaa tattgtgcgg aatacctact
     9361 acggcggctt cggcaatctt cggatgcgcc accagcgccg actctatctc tgccgtcccc
     9421 agacggtgac cggagacgtt cagcacgtcg tccacacgcc cggtgatcca gtaatagcca
     9481 tcttcatcgc gacgcgcgcc gtcgccgctg aaatacatat ttttgaaggt ggaaaaatag
     9541 gtctgctcaa aacgttcgtg atcgccaaac agcgtacgcg cctgaccagg ccaggagtcg
     9601 gtgatcacca ggctaccttc ggtagcccct tccagcgggt taccttcgtt atcgaccagc
     9661 gccggttgca cgccgaagaa cggacgtgtt gccgaaccgg ctttcagctc ggtagcgcca
     9721 ggcagcgggg tgatcatgaa accgccggtt tcggtctgcc accaggtatc gaccaccgga
     9781 catttctcgt tgccgatttt tttccagtac cactcccacg cttccgggtt aattggctcg
     9841 cccacggaac cgagaatgcg tagcgacgaa cggtcggtgc cttcaatcgc tttatcgcct
     9901 tccgccatca gcgcgcggat cgccgtgggt gcggtataga gaatattgac ctgatgcttg
     9961 tccaccacct gcgccatacg ggcaggcgtc ggccagttcg gtacgccttc aaacatcagc
    10021 gtggtcgcgc cgcaggccag cgggccgtac agcaaataac tgtgtccggt aacccagccc
    10081 acatcggcgg tgcaccagta gatatcaccc ggatgataat caaagacata tttaaaggtc
    10141 agcgccgcgt acaccagata accgccggta gtgtgcagta cgccttttgg ttttccggta
    10201 gaaccggagg tatagagaat aaacagcgga tcttcggcgt tcatctcttc cgcctggtgc
    10261 tgatcgctgg cttgctcaac caggtcgtgc caccacaggt cgcgcccttc ctgccagtcg
    10321 atgtttccgc cagtacgctt cagtaccacc acatgctcta cgctggtgac gttcgggttt
    10381 tttagtgcgt catcaacgtt tttcttcagc ggaatactac gcccggcgcg cacgccttcg
    10441 tcggaagtga tcaccagtcg tgagttggaa tcaataatgc gcccggcaac ggcttccggc
    10501 gagaagccgc cgaaaatcac cgaatgcacc gcgccaatgc gggcgcaggc cagcatcgca
    10561 accgcggctt ccggcaccat cggcatataa atcgccacca catcaccttt tttaatgccc
    10621 agctcgagca gggtattggc gaagcggcag acgtcgcggt gcagctcttt atagctgata
    10681 tgtttgctct ggctggcgtc gtcgccttcc cagatgatgg cggtgcgatc gccgttttct
    10741 tgcagatggc ggtcaaggca gtttgccgcc agattcagcg tgccgtcctc gtaccattta
    10801 atggacacat taccgggggc aaaggaggta tttttcacct tctggtacgg tttgatccag
    10861 tcgagaattt ttccctgttc gccccagaag gtatcaggta cgttaataga ttgttgatac
    10921 atcgcctcgt actgctgagg gtttatcagg caacggtctg cgatgttggc aggaatggtg
    10981 tgtttgtgaa tttggctcat gcttttgttc tccttgtagg atgttaataa tatgtggcat
    11041 aagcgttaaa tgtaggggta ttggcagttt tgtttagtat ttgggcgaca gatcacgcaa
    11101 aagtagaatg gtgcaaataa acggcagggt aatttttgaa ggtcaccaac aaaagttgat
    11161 taattctttg aggaacatgc agttatgcat gctgttgaaa aagaggaaga tactgactaa
    11221 ctctaaagtg gtatttacat gcacttacaa ttgattaaag acaacattca cagtgtggtt
    11281 atttgttaca cataggggcg agcaatgtca tgacagtgta ggtgcggtta ctgtcgtgaa
    11341 aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc tatgacaagg
    11401 ataaaaataa acgcacgccg tatcttcagc ttattgattc cttttttctt tttcacttct
    11461 gttcacgctg aacaaacggc agctcccgca aaacctgtaa ctgtggaagc gaagaatgaa
    11521 acctttgccc cgcagcatca cgatcaatat ctctcctgga aagccacctc ggaacagtca
    11581 gagcgtgttg acgccctggc ggaagatcca cggctggtga tcctgtgggc ggggtatccc
    11641 ttctcgcgcg attacaacaa gccgcgtgga catgcctttg ctgtgaccga tgtgcgtgaa
    11701 accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat ggcgtgctgg
    11761 agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag acggcgaaga tggctacttc
    11821 cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg tgccgactgc
    11881 cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct ttcccgtccg
    11941 tatgcggctc gcgcgatgga agccattggt aaaccttttg agaaagccgg acgtttcgac
    12001 cagcaatcga tggtttgcgg tcagtgccat gtggagtatt acttcgacgg caaaaacaaa
    12061 gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa atatggagca gtattacgac
    12121 aaaattgcct tctctgactg gactaactcc ctgtcgaaaa cgccaatgct gaaagcgcag
    12181 cacccggaat atgaaacctg gacagcgggc attcacggta aaaacaacgt gacctgtatc
    12241 gactgccata tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga ccataaaatt
    12301 ggtaatccgt ttgataactt cgcccagact tgtgcgaact gccataccca ggataaagct
    12361 gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta acgacctgaa aatcaaggtt
    12421 gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg cgctggatgc aggcgcgacg
    12481 gaagccgaaa tgaagccgat tcaggacgat atccgtcatg cccagtggcg ttgggatctg
    12541 gcgatcgctt cccacggtat tcatatgcac gcaccggaag aaggtctacg gatgctcggt
    12601 acggcgatgg ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct ggcgaccaaa
    12661 ggcatcaccc atgaaatcca gatcccggat atctcgacca aagagaaagc ccagcaggcc
    12721 attggcctga acatggaaca aatcaaggct gagaagcagg acttcatcaa aacggtgatc
    12781 ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa gccaataacc ccgttccgcc
    12841 tcgcaagggg cggaaaacac aatggagtga atatgagcgt attacgttcg ttattaactg
    12901 ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct acccctgcgg
    12961 tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg taacccggat gccgcctgtc
    13021 tggactgtca taaaccagat accgaaggta tgcatggcaa acatgcttcc gttatcaacc
    13081 cgaataacaa attgccggtc acctgcacca actgtcacgg ccagccatca ccgcaacacc
    13141 gcgaaggggt gaaagatgtg atgcgcttta acgagccgat gtacaaggtc ggagagcaga
    13201 acagcgtctg tatgtcctgt catctgccgg aacagttgca aaaagcgttc tggccgcacg
    13261 atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca cagcaagata
    13321 cgatgcagac gttaagtgac aaaggacgga ttaagatttg cgtcgattgc cacagcgatc
    13381 agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt gcttaaggag cagccatgac
    13441 ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca gcggcaccgc
    13501 agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg cgttacggca tggtgcatga
    13561 cgagtcgtta tgcatcggct gtaccgcctg tatggatgct tgtcgggaag tgaacaaggt
    13621 gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg aatttcctga
    13681 tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc gatcatgcgc cgtgcgttga
    13741 cgtctgcccg accggtgcgt cttttcgtga tgccgctagc gggattgtcg atgttaatcc
    13801 tgatctctgc gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg tgcgctttat
    13861 ccatccggtc acgaagacgg cggacaaatg cgatttctgt cgtaagacca atttgcaggc
    13921 cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt ttggcaatct
    13981 ggacgatcct aacagtgaga tttcgcaact gctgcgccag aagcctactt accgctacaa
    14041 gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg gggaggtgag
    14101 ccaatgacgc agacttccgc atttcatttt gaatcgctgg tgtgggactg gccgattgcc
    14161 atctacctgt ttttgattgg tatttctgcc ggtctggtga cgctggccgt gctgttacgt
    14221 cgcttctacc cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac gctgattgtc
    14281 gggccgggcg cggtgatcct cggtctgttg atcctcgtct tccacctgac aagaccgtgg
    14341 accttctgga agctgatgtt ccactacagt tttacctcgg tgatgtcgat gggggtgatg
    14401 ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt tgaacatgat
    14461 ttgcttgccc tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa ggttctgagc
    14521 ctgctgacgc ccgttcatcg cggactggaa acgctgatgc tggtgttggc ggtgctgctg
    14581 ggggcttata ccggctttct gctgtcggcg ctgaaatcgt atccgttcct caataacccg
    14641 agcctgccgg tgctgctcct cttctccggc atctcgtccg gtgcggcggt ggcgctgatc
    14701 gccatggcga tacgccaacg cagtaacccg cattccacgg aagcgcactt tgttcaccgg
    14761 atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt tgtcggtctg
    14821 gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg cattaggtgg cggtttctgg
    14881 acgtggtggt tctggcttgg tgtcgccggg ctggggctga ttgtgccaat gttgctcaaa
    14941 ccgtgggtca atcgcagttc cggcattcct gccgtgctgg cggcgtgtgg ggccagtctg
    15001 gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg gacagttgac ggtggcgtaa
    15061 gccagaaaag aggtggtttc tggacgtatt ccttcctgaa gtcggttttc tggcgttgtt
    15121 gttaagtctc ggggtcaacg tgttgacccc gttgacggcc tttgcgggag tgcggttgcg
    15181 ctggcctgcc atgatgcgac tcacttgcat cggcattctg gcgcagttcg cgctcctgct
    15241 gctcgccttt ggcgtactga cgtattgttt tctcatcagc gatttctcgg tcatttatgt
    15301 cgcccaacat agctacagcc tgctgtcgtg ggaactcaaa ctggcggcgg tgtggggcgg
    15361 tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc gcctggagcg cgctgtttgc
    15421 ctggcattat cggcagcaaa gcgatccgct atttccgctg acgctagccg ttttatctct
    15481 catgctcgcc gcactgctac tgtttgtggt gctgtggtcc gatcccttcg tgcggatatt
    15541 tccaccagca atcgaaggcc gcgatctcaa tccgatgctg caacatcccg gtcttatctt
    15601 tcatccaccg ctgctttacc ttggctatgg cggtttgatg gtagcggcga gcgtggcgct
    15661 ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct ggcgctgggc
    15721 gttacctggc tggagtgcat taacggcggg gatcatcctc ggttcctggt gggcctactg
    15781 cgaactcggc tggggcggct ggtggttctg ggatccggtg gaaaacgcct ctttattacc
    15841 ctggctttct gccactgcgc tgctgcacag tttgtccctg acacgccagc gggggatttt
    15901 ccgccactgg tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc tgggcacctt
    15961 aattgtccgt tctggcattc tggtttcggt tcatgcgttc gcactggata acgtccgcgc
    16021 tgtgccgttg ttcagcctgt ttgcactgat tagccttgcg tctttggctc tgtatggctg
    16081 gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg aaatgttaat
    16141 cctcgctacg ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg gaacgcttta
    16201 tccgatgatt tacggcctgc tgggctgggg acgcctctcc gttggcgcgc cgtattttaa
    16261 ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg attgtgctgg cgacgtttgt
    16321 ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc tgttatttgc
    16381 cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc agcctgaatt tacagccggg
    16441 tcagcaggtg acgctggcag gatacacctt ccgttttgag cgactcgatc tgcaagccag
    16501 aggcaattac accagcgaaa aagcgatagt ggcgctgttt gaccatcagc aacgcattgg
    16561 tgaattaacg ccggagcggc gtttttatga agcacgccgt cagcaaatga tggaaccgtc
    16621 aattcgctgg aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa ccagagcgga
    16681 tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg ggggaggatt
    16741 gttgatgatt gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg atgaataaag
    16801 ggcttctcac gctactgctg ttatttacct gttttgcgcg cgctcaggtt gtagacacct
    16861 ggcaattcgc caatccgcaa caacagcaac aggcgttaaa tattgccagc cagttacgtt
    16921 gtccgcagtg ccagaatcaa aacttactcg aatccaatgc gccggtggcg gtcagtatgc
    16981 gccatcaggt ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc attggctgga
    17041 tgaccgaacg ctacggagat tttgttcgct ataacccacc gttaacgggt cagacgctgg
    17101 tgttgtgggc gctgccagtg gtgttgttat tgctgatggc actgatcctc tggcgagtga
    17161 gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac tcacgatatt
    17221 gatggtattc ctctgtgtcg gcagttatct gttaagccca aaatggcagg cggtacgtgc
    17281 ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa ccccggaagc
    17341 gcagcttcag gcattgcagg ataaaatccg cgctaatcca caaaacagcg aacaatgggc
    17401 gttactgggc gagtactatc tgtggcaaaa cgattacagc aattcgctgc tggcgtatcg
    17461 tcaggcgttg caactacgtg gtgagaacgc tgaactgtat gcggcgctgg cgacggtgct
    17521 ttattaccag gccagccagc atatgaccgc ccagactcgc gcaatgatcg acaaagccct
    17581 cgcgctggac tctaacgaaa tcaccgctct gatgctgctg gcttccgatg cgtttatgca
    17641 ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg atggatctca actcaccccg
    17701 gatcaaccgg acacagctgg tcgagtcgat taatatggca aaactgttgc agcggagatc
    17761 ggattaatca tctggtgatg aagattatgt cattgagctg cataaaaaat aatcgaatga
    17821 acatatgcca aaaataatca ccaatcagta ttattgcaga ttaaacaaat aaaaatcttt
    17881 ccataacaaa tggttattca ttaatcctgc cataaactcg cgttattatg cattaatgca
    17941 gcgaaaagct ctgttgttaa agggttgcgc aacataccgc gcaaatgata ctgatcataa
    18001 gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg ccctggcagt atgtcacgtt
    18061 ctcgcgtttc tgaacgggga acggcgctcc attgaggaag ttattctgat gaaaaatata
    18121 aaattcagcc tggcctggca gattctgttt gctatggtgc tgggcattct cctgggaagc
    18181 tacctgcact accatagcga cagccgcgac tggctggtcg tcaatttgct ctctccggcg
    18241 ggtgatatct tcatccatct gatcaaaatg attgttgtgc cgattgtgat ctccacgctg
    18301 gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg cgcgaaaacc
    18361 attatttact tcgaggtgat caccaccgtc gccatcattt tggggatcac tctggcgaac
    18421 gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga tatctcgaaa
    18481 tatcagagca ctacggaagc ggtacaaagc agttcccacg gcattatggg cacgattttg
    18541 tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct gccgatcatc
    18601 tttttctcgg tgctgtttgg tctggggctt tcttccctgc ccgcgacgca tcgtgaaccg
    18661 ctggtgaccg tgttccgctc catctctgaa accatgttta aagtgactca catggtgatg
    18721 cgttatgcac cggtgggtgt gtttgcgctg attgcggtga cggtggctaa ctttggtttc
    18781 tcgtctctgt ggccactggc gaaactggtg ctgctggtgc atttcgccat tctgttcttc
    18841 gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg gatcctgatt
    18901 cgtattctga aagatgagct gattctggcg tactccactg ccagctctga aagcgtactg
    18961 ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac cagcttcgtg
    19021 gtgccgaccg gttactcttt taaccttgat ggttcgacgc tgtatcaaag tattgccgct
    19081 attttcatcg ctcagctgta tggcattgac ctgtccatct ggcaggaaat cattctggtg
    19141 ctgacgctga tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc gtttgtggtg
    19201 ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt tattgctggt
    19261 gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa tgcgctggcg
    19321 gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct ggcttatgag
    19381 cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat aattgaagat tgccggggat
    19441 atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc aggcctacct
    19501 taaccgttgg ttaatttttc aaactcttca cacccggtat caaacccttc catacagctc
    19561 aggttcaacc agtgcagcgc cttttgtttg ttcttctcga taaaaccttc ctcaccgttt
    19621 aagaacatca ttcccgccca gtactcggaa taaccggttc gggaaattgc agagctgcgt
    19681 ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt ggcgtaaatc
    19741 aaaccaagca gcatttgggc atcgacggcg gagtcattct ccagatcttc cgaggcgttt
    19801 tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt taccagaata
    19861 tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc tgcaagaact
    19921 tttgcttgtg gatagtccag actgaccgga ttggtgattt tgatctgcgc caacagcgcg
    19981 caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc tttgctcaaa
    20041 tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgtcggtc cccggcctct
    20101 gctgccttta aatactggct gccaggctcg gagtcgttcg cgtgggcaaa gaatgtcaaa
    20161 aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag ggagtaacca
    20221 gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta gaaaggacag
    20281 tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc gtcaatcacg
    20341 tactgctcgg cggcgcgctg atcggcgatc ggctcgacgc gcacggcgca gtatttgtat
    20401 tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca ggcaccaatc
    20461 caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac ctgcgcacgg
    20521 gtgataattt tgcctttacg cgagtgcact caaaccaatg cctcatcttc aatacccaga
    20581 cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag cgccgccagt
    20641 gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg caccgttgac
    20701 agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc gcaggtgaag
    20761 aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga agtcccctga
    20821 tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc cattttctcg
    20881 taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat ctcctgggtg
    20941 ttgttgtagt gcatcggata accaatacgg gtggcgattt cactgatgat ttgccagtcc
    21001 gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc acggtcagcc
    21061 gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac atccgccgcc
    21121 gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc ttcaaaggct
    21181 ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat aatgtacgcg
    21241 gcacgcactt cgccatgcgc tgcgcggtgc ggcaactcgc tgatgcgata gccggtatga
    21301 gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc cggatctttc
    21361 acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc accctgcacg
    21421 ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt accggtcagc
    21481 atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg ggttacaccc
    21541 atgccctaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc ctgacgaatc
    21601 tcactggcgc tgacaccggt gatatcttca accgactccg gcgtgtagcc ttcaacgatt
    21661 ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc gtacagattt
    21721 tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc gtttttcagt
    21781 gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca gacgataatt
    21841 ttcgccccgt tacgtttagc gttaattacg tgattcgcca cgattgggtg ggaatccgcc
    21901 gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat agcattgctc
    21961 attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg tcagacacga
    22021 gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg cattacatag
    22081 ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc cggaccgtac
    22141 ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc tcaggaaaca
    22201 ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg cggggtcagg
    22261 atctgggtat cgttaatgaa atcccagcca taataaccct tcagacacag ggtaccctgg
    22321 ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac gaccaggttg
    22381 attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat cggtctcgct
    22441 ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt tatgccacat
    22501 tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc tgacgaaaaa
    22561 caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc atcacgcggc
    22621 agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc agcgatttca
    22681 tcaactgaat gctttggatc acccggcggc tgtccagcat cagtaatgac atctcttcgg
    22741 caagtactgg caaccgggct tcagtggcct gtaagcggct ggttaagccg cgctgatagg
    22801 cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc tgcatttctc
    22861 gctcgtcgtt gagcgtttgc agatgcgtgc cgttgacggc aacgtcacgc accgcgttca
    22921 gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg ccttcgagat
    22981 tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg aagttgaact
    23041 ggcgactggt ttttttgaat aaggtatcca ggtggatggc atcaaggccg aaaaacgctt
    23101 tgatatcaaa gctcggatag aacaacgccc gcgcggaatc cacctgatct aatgacgcct
    23161 gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca taagagagtg
    23221 tcgccggaat gccggtctgg actcgtggta atgccacagg tttgatctcc ggcatatcgc
    23281 tggctcccgc gccaatcaat gcgcgcagag attctctcgt ttcggtgatt tgccctttga
    23341 cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg aaaggcactt
    23401 gtgcttccag accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa tcaatcacat
    23461 cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg taataaagct
    23521 gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc gcggcattat
    23581 gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca agatccagtc
    23641 cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccatcccc agtgcgggcg
    23701 catccatcgc ataagggctt aaaaagccgt tcgtcgagac acgttggcgg ttaagcatcc
    23761 ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa atcggcctgc gattgcgctt
    23821 tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc cgttggatca
    23881 gggcatccaa ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc cagccggagc
    23941 tggcaagatg aatatcatcg gccagtttga tttgttccgg tttgagctgt tgatgaggcg
    24001 cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg agaatgctgc
    24061 acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg cctgttccgt
    24121 ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct ggcagacgtg
    24181 ttgctcctgt tttaataagg tggcaggatg gcctgcgagg cgggtgtctc ttcaagcgtt
    24241 atttgaggag aacggctgag tgcggttgcc agaccggcag cgtatttctc cagggcgtcg
    24301 gcaaactgac ctgctctgtc cggctgtagt gcgttatcca gcgcctgcgc caaattccag
    24361 gtggcatccc aggcgtgaag gatatcgcgg ccctgacgaa taaccgtttg cgaacgttca
    24421 atcagtaagg cgcgttcttc gctgtccagt tgacgctcca gcgccacgcg ttggcacatc
    24481 tcttcacagg cattaaacgc cgcatgcaga ccgatacgaa tttgcagata agtgcgcaga
    24541 gccgtgactt cctgctggcg tgggatccgc attactttac ttaacatacc cagcgcgcca
    24601 gccagttttt gcggcagcgt gcgcgcttca ctttcaggcc agacaaaggt gtaaatcatc
    24661 gcggacacca cggtaccaat gatgataccc aaggcgcgat cgcgaatttc caccaggtcg
    24721 tacactgggc caaaaacgtt ttcgagcgtg gcgagcgcga aggtgaccac catctgtgtg
    24781 ccgatataag aagagcgttc agagctggta gcaatccatg cgcccaacag gaaaatcggt
    24841 gccagcacaa acagcaattc gacaatattg tccagccagg gcatgaccag tagcgtgaat
    24901 aacagcgcca gaatcgcgcc gcaaaaggcc ccgccaaaac gcagcaccat cttctggtac
    24961 gacgaaccga catttggatt agcgacgatc acgcaggtca gcatacaggt gtgaatgcct
    25021 tcccagtcca cgccgctgta aaaggtgtaa cagatcaaac aggcgagcaa tgtttttacc
    25081 gcgtagcgta tatagtctgg attggtaaaa gcatcggcga ccattgatgg cggtttggcg
    25141 gcgggcgttg gcggcgtatt cgggttcatc tggcccagtt gtaacaacgt ctggcagata
    25201 ttctccaggt tacattcccg tgccgccacc gcttcacttt cactgatccg ccagtcgctt
    25261 tgccagcact gaccttcagc aacggcatgc tgcagcttgt tgatttctga ggctaatttt
    25321 tgccgaaatt caataattgc ctgagaatca gcaaaagagg tgggatcgta gcgattcagc
    25381 gtcgagtaaa tgtaggttac cgttgccacg cagctttgcc accatgcgct ttgagttcgc
    25441 cagttggcat cgtccgcgag acaaaagaca ttcagttttt gcagcgccag cgcctctctt
    25501 tcaatccgcg tttcgggtag cggtgcgagg ctgtccgtca ggtggctaat ggcatcatca
    25561 agccgatcat taagcgcttg atgcatttgc gtaatggcac gactgggaaa ccacagcacg
    25621 ccgattaacg tcatcagcaa ggttggatag aggccaacaa cgatacacca cagcgttaag
    25681 cgcacgacta cttccggata gtcgagcatg gcggggaagg tttgcccgta aatggcgaca
    25741 atggcgacag cgaaaaagac cagtcccagc cgatgggtgc gcatcaaaaa catgcagccc
    25801 atcaggatcg gcccggcgat gatcaatcgg atcaacggtt cgccgtatga ccatttgtag
    25861 atcaaaaaca ggctgccgat ctccagcacc gtggcaacca caaacaagat cgccacaaat
    25921 ttggtgtaaa acgcgttcga ctgaataccg taaaacagca ctgccagcga taacgccaca
    25981 aaagggatct caaaagtcat cgagatcaga atcaccagca ggcagcctac ccagagttgc
    26041 acgatctgcg gcacgcggcc gggtcgccgc tcgcttaact cttcatgaaa gaacgccagc
    26101 agcctgacca ccggtaatgg cagggagttg agcgcgctca ttattgcggc tcaagattag
    26161 cgactgccga agcgccgatg cggaacattt ccgggtcagg tttatcgact atgattttga
    26221 ccggaaaacg ctgggcaacg cggacccagt taatagaacg agacactttt ggcaggccgc
    26281 ccagcaccag gccgccgtca tccggtagca cgccgtagcc aatcgaatcc actttaccct
    26341 cgaaggtttt gccgctgtca ctcatcaggc gaatcgttgc gggtgtacct gagcgaatat
    26401 ttttcagatc ggtttcgcgg aagttggcga tcacgtacca gtgacgagtg tcgatgaggg
    26461 taaaaatagg gcgcatggca gaagcaaatt gcccgacgga ggttttgagg gaaatgatcc
    26521 ggccatcaaa tggcgcgcga acggtcgtca tttccagatg tagtttggtc agggcaatat
    26581 ccgcttcgac cgccgcacgt tgcgccacca gcgcatcgac gccgctggcg gctgactgcg
    26641 cctgtaacaa tacggcatta agatccgctt ctgcggcgcg ctgcgccgtt cttgcacggt
    26701 caacgtcttc tgctgagaca aaaccctctt tcagtaatgg ctcggtgcgg cgtaatgtat
    26761 ctgtggcctg tttcgcggcg gcacgggctt tttctaccgt ggcattaacc gagtcggcac
    26821 caaactgttg ggcgtcaacg ctacgctggg tgagcataat ttgcttatcc agcgccgcga
    26881 gggaggcttc ggctttcgcc aggttggctt cgtacgggcg cgggtcgatg cggaacagca
    26941 aatcgccctg tttcaccgcc tggttgtcgg tgaccgccag ttctacaatg cgaccgctga
    27001 cttccggcac cacatcaatg gtatctgctg acgcgtaagc gtcattagtt gatggcgcac
    27061 tgtctacgcg ccagataacg aaaacaaggg caaccagcgc caacgcaacc actaacagag
    27121 cagggaattt actgcgagga gcttttttcg gcgtactttc catgagcgcg tctcaaatag
    27181 attagaaaaa tgccagccag aacagcatgg cgtagagagc aaaaagggcg gtataaataa
    27241 ttccgacaaa tgccagattg atatttgccc gctgaataac tcgcctcgtg atgagcgtca
    27301 aaataaggct ggcaatggcg cagaaaaacc agctgggata ataagcgccg atcaccggga
    27361 ttgccggaga taatgagcat cccgaaacca tgaagacggg aattatccag gctgtttttt
    27421 tgaggtgcat cgccatgcga gagagaactg ttggcatatt aaatacatcc tgtctgctgc
    27481 gaacaatatc tgtagtgaat atcaatatcc attgaaggcg tcagtatggc tattttctcg
    27541 atgaataaaa atagctttat ttagccaccg cattcataac ttatatattt tatttattaa
    27601 ttggctgtat atatttttaa aataaatgct tttgggggct tcagcgcccc caaaagcgag
    27661 tgacattaat ttggggttac gatattcacc cacaaatcga aattatccag acaggcgata
    27721 atttcttcca gtttcgcgcc attgccaata attttggctc tcttcgcttt caccagatcc
    27781 gccattttgg cctgtccggt cagcacggcg tgcagatctt cacggctgat gtagaaagag
    27841 gcgtcgactt gcggttgcag tgttttacgg tagttaagca cgctatcgtt cagcgtcagg
    27901 ttgaggttat cgccgttgct catattgaag ttcaggctga tatttttacc cgcagctttc
    27961 gcgctatcga ggcgaacggc cataaagtcg aacagcattt cgaccgacat cccgcgaatg
    28021 gtgtccgggg aaccggtggt gccgtggctg aacttatgca ccccttcgcg cagctctttc
    28081 gcgccggtca ggtagaaacc gcgccatgtg gcggactcgg cctgatagcc cagctgttca
    28141 aagttattcg cttgcagatt ctttgcgacc tggtcacccg ggttggcggc aatcacctgt
    28201 ttcagcagtt ctgccgacca gcggtaatca ccttgcttgt tcgcttcttg cgccaggtta
    28261 ataacgcgtg cggaaccgcc cagcacctgc acgtaacgtt tacccatctc cacctgacca
    28321 tacggatgca ggttagccgg gttaccgtcg taatagccaa gatagaagtt atacaccgcg
    28381 cgggcgttgt ggctgacaga accgtaatag ccacggctgg cccagttatt ggcaagtgca
    28441 ggcggcagtt taatcatgtc gccgatttca ttcatggtgt agccctggtt cgccaggtgc
    28501 agggtctggt cgtgaatgta cttgatagta tcgcgatatt taccaatata atcattgata
    28561 tgcttattgc cccagaccgg ccaggtgtgc ggcataaaca ggacttccgc gtcgttaccc
    28621 cacatatcca gcgtttcgtt cagatactcg gtccacttgc tggtgtcgcg ggttttcgcg
    28681 ccgcgcagag tgtagaagtt gtgcagggta tgcgtggcgt tctcggcggt acacagcgct
    28741 ttcagggccg gaatatagaa gtgcatttcg gctggtgctt cgctacctgg ggtcatcagg
    28801 aagtcaaact ccaggccgtc gataatcatc ttctcgccag ttctgacgat agttttagtc
    28861 ggtgcaataa tgctcgggtc gcccgttgcc agcgtcacgc caaggccatt acccacgttg
    28921 ccttgcgcgt tgtgcggcag taacagaccg taagagtaga gcgcacggcg gctcatgatg
    28981 ttccccgcca gcacgttttc gctgatggct tcgtccataa agcctgctgg ggcaatcacc
    29041 tgaactttgc ctgatttaac atcggcttca gagataatgc ctttcacgcc gccatagtgg
    29101 tcggtatggc tgtgggtata aataaccgca acaatctgtt tttgcgggcg attttggaag
    29161 taaaggtcaa gggcggcttt tgcgctcggt ggcgtcacca gcgggtcgat gacaataatc
    29221 ccggtatcac cttcgataaa ggtgatgttg gagatatcct gaccgcgaac ctagtacatt
    29281 ctgtcggtga ctttaaacag gccggaaata ccgtttaact gcgactgacg ccacaggcta
    29341 gggttaacgg tttcgggcgc gggcgcattg atatcaaatt tataatcttc cccgcgataa
    29401 tatggtttgc cattagcatc gcgcagaata ccttcatcca gcagcggagc gataaatcca
    29461 cgctgagcgt cgtcaaaatc ctgacgatcg ctaaacggta atgatttggc gtaattctga
    29521 ttaacctgtt gggtatattg agtggcggct ttcgcttcct ctttcgcgtt agcggtattt
    29581 accatcatgc cgctggcggc agttaacgca ataacaattc tgctcaaacg gaataaccga
    29641 gagttattca tataaatctc catttatcat ttgtgtcgta aatatgtaat ccatgcaaaa
    29701 tacgtcattt aaagctgggg ttatcataat tgggaatttg cgactttaat aagtggaagt
    29761 gtgagcggaa cgcgccattt tattaggcat ttgtgctgaa gagggaaaag cacggcatat
    29821 ttattactat catcaataat gtgacctgac ttatattttt attatttata ttactttccg
    29881 ctttcacttc gacaagaagt acgcacaatg attaattaga tgaatgatgc ggagtgtaac
    29941 catgaaaccc gtcgtgctgg tggtggatga tgacacggct atctgcgcac tgttgcagga
    30001 cgtgttaagt gaacatgtct ttacggtttc agtcagccat accggacagg aggccatatc
    30061 gcgtattgag ggcgagcctg acattgcgct ggtggtgctg gatatgatgc tgcccgatac
    30121 caacggcctg agggtattgc agcaaatcca gaaactgcgt ccggcattgc ctgtggtgat
    30181 gttgaccgga atggggagtg agtctgatgt ggtggtcggg ctggaaatgg gcgcggatga
    30241 ttacatctgc aagccgttca caccacgggt ggtggtcgca cgactgaaag cggtgttgcg
    30301 atgggttggc gcgctggcgg ttaatgagga gaaaaacggc gggctgtcat tcaatggctg
    30361 gtatctcgat accattcgct gccaactgca tagtccgcta caacaacatg tcgagctgac
    30421 tcagggcgaa tatggcttgt tactggccct ggctcaaaat gcccgtcggg tattaagccg
    30481 cgaacagctg ctggcgttca ctcatagcga cagcgtggag gtgtttgatc gcaccatcga
    30541 cgtattaatc atgcgcttac gacgcaaaat cgaactcaac ccgcatcagc cgatgttgat
    30601 taaaactctg cgcggtctgg ggtatgtctt cgctgccgac gtgcattaat ccgacatttc
    30661 tgtttgcagc tcttccagcg ttttcgcccc gtcgatggca ttggcgcata acaggcttgc
    30721 tcgcgcgcag gcgtgtgcca gtttgatgct ctcggtaagc gtccagcgtt catggcagcc
    30781 atataaaaat ccggcacaaa atgcatcgcc cgcaccgacg ctgccgataa tttcccgttg
    30841 ttctaacttt tgcgaaggga tccacacgcc gcactgttcc ggggtctctc cccacgcccc
    30901 ttccgggcag tggatcacca cacgctggcg cacgcccgca tctaacagtt cacgcgcggc
    30961 ggcagcaata tgcgcgatat gcggttcgcc gttgggtaac cgaatttcca gcccactaaa
    31021 ctccccggcc tccagttcgt taattaccag gtaatccaga tggcgcaacg cgggcagcac
    31081 cagcggctga taacgcggat cgcccttgcg ggagaccagg tcgagtgagg tttcaaagcc
    31141 agtatcacgc atttgcgcca gcaggcgtgc gctacgggtg ccgtaaacat cgtccggcat
    31201 attcaggctg tcgagcaaca gtaaatagcc gagatggaaa atcttcatgg tgttatcgag
    31261 ctgatcgaac gccggaagat ccaaaagtct gtttgcacct ggcgaatgga aaaacgtccg
    31321 ctgcccgctt ggatcagtca ttacctgcga cattgacgtc ggggcaaagg tagtgcgctg
    31381 cacatgctga cgattgacgt gatattgctc aagcatcgcc ataatgtaat cgccgtcgct
    31441 gtcctgaccg attagcccga ccgcctgtaa cggcaacccg gtgcgcattt tcgccagtgt
    31501 cagcaggacg ttaagcggcg cgccgccggt ggagcgttcg ctgtgggtaa tctccgccag
    31561 ccagccgcgc tctggccact ggataatctg gtggacgtgg tcaaccagca tattgcctgc
    31621 ggcgataatc cctttacgtt ccatcatgcc tgccccgcac tgccgaaaat acgcatctgt
    31681 tgtgcaaccg tatcgctgat ggcggcttcc actgctagta gtaactcggc aaactcgtca
    31741 tacatcggct gacggtgcgc cattcgggcc tcaacggcag ccagcgccgc ttgtgacatg
    31801 ccggtgtaga aattgatttt gtggataccc agttcgatgg cgcggcggaa atcggcatct
    31861 ctaatccctg aaccgccgtg taataccagc gggatagccg cctgctggcg gatagcgtcg
    31921 aggcgcggga aatcgagttt cggttcgccg cgatatttgc cgtgcgcatt gccaatcgcc
    31981 acggccagcg cgtcaatgcc ggtacggtcg acaaaatcac gcgccagctg cggatcggta
    32041 aacagagatt catcggcatg accgtaaagc gcaccgcctt cgtcaccacc caccgcgccc
    32101 agttcagctt ctaccgatac gccaacggca tggcacattt tcaccacttc ccgcgtctgg
    32161 cggatattct cttcataccc cagcgtggag ccatcaaaca ttaccgagct gaatcccagc
    32221 cgcaacgctt gcgccaccga ttcaaaatgc agcccgtgat cgagatttaa taccaccgga
    32281 atcgcttgac gcgccgcttc aaatttcact gtctcgacca gggaatccag cgagatatat
    32341 ttaaaatgta cttcggcaat gttgataata aagggcgaac gttcttgtct ggcagcagca
    32401 aacaaggcgc gtaaaaagtg tgagtccagc acattaaacg cacctaacgc atagtgatgt
    32461 tcgcgggcat gtgccagccc ttcagcgaga gaaatcaacg gcataacgtt atccttattc
    32521 agaggaactg gcggtattcg ttacacaaca ccagctgggc cggttcgtct tcgtcaatca
    32581 ggttgtagcg gtccagcggt tgtaaaaagt ggttatcgtg gtcgtcgtca ttgacagaag
    32641 agacttcgcc aaccagaaca tcgccaaaac cggtttccgc ccaaaagctg tgatacaggc
    32701 cgggcggcag gcagatactt tcgccggggg acaggcgtaa ctgactgcct gcgacatgag
    32761 tctggcggca gccgtcgatc accaccgtaa cgtcggtatc aactgtttgc tcgttgctat
    32821 cggcattcca cagttcaaca atcaaattgc cgccgccgcg attgatgatg tcctcacgct
    32881 tacgccagtg aaaatgcatt ggcgtgacct gcgcatcgcg cacatgcatg atcttctcgg
    32941 cgtaacattt cacatacggc attccttttg ctgagccgtt acgcagggta aacagcgtca
    33001 gcccctgtgc tgcaaagttg ttaccaccaa aagcggtaac atcccagccg agtttgagat
    33061 caaaaacctc ctcccacgcg gcggtgtcga gctgctgcca ttgagcagga gagaaactgg
    33121 caaagggcgg cagatgtacg tcatgttgcg aaaagaattg ccgtgtatgg ccgagaatgt
    33181 tgtttatagc ggagcgtttc atcgaaagcg ttctccttag cggtggccgg gcagcatcgc
    33241 cgcccggcgt gtggacttat ttcaccgtcc agcctttgta attgccgacg ttgtttttat
    33301 cgatcatcgt caccgggatc agcaccggct ctttcggcgc aggtttgccc tgcaaaatgt
    33361 cataaccgat ctcaaccgct ttcgctgcca tcacctgtgg atcttgcgcc ggagtagcga
    33421 caaacaacga gttctcacgc ttgagcgcct cttcggcgtc cgggcttcca tccacgccga
    33481 caataaagaa ctcgttacgt tgcgcctgtt ttgctgccag atcggcaccg atcgctgtcg
    33541 gatcgttaat cgcaaatacg ccgtcgatct tcggatttgc cgccagcagc ccggtcatca
    33601 cctccagccc gccctcacga ctgcctttgg cgttcttgtt gtcagagagc accttgatgt
    33661 caggatggcg tttaaattcg gtctggcagc cttcgacgcg gttttgcacg gcggacaccg
    33721 gcggtccgtt gatgatcacc acgttgcctt tgcctttcag gcgatcggtg atgtatttac
    33781 aggccatttc gcctgcctgg gtgttatcgg aagtgatggt ggcatcggca ccttctgccg
    33841 ccacgtcaac cgccaccacc acgatcccgg cgtcttttgc ccgtttcacc gccgggccga
    33901 tcccttttga atcggcagcg ttaaggatga tcatatcgac gttagcggcg ataaagttgt
    33961 ctatctgcga gacctgctga cccagatcgt agccgctgga aaccagcgtc actttgacgt
    34021 tatcgcccgc cagtttgcgt gcttccagct ccgcgccttt ggtgatctgt acgaagaacg
    34081 ggttcgccag gtcacctacc gttacgccta tcgatttcag atctttcgcc tgggcgaacg
    34141 gagctgcggc cagcagcgcg cctgcacaga gcgcggtcac taagggcttc aatctcatgc
    34201 tgtattcctc atgtgtaggg tattgttgtt atgcactttg atgatgtcgg gtacggtatt
    34261 tgtcgataag caccgcaatg atgatcaccg cccctttgat caccagctgc cagaagtaag
    34321 agacgcccat taaggtcatg ccattgttga gcgtggcgat aattagcgcc cccaccagcg
    34381 tgccggtgat ggtgccgatg ccgccgacaa aactggtgcc gccgaggatc accgccgcaa
    34441 tggcatccag ttcgtaaccc atacctaagt tgccgttggc gctgtacagc cgcgaggtgc
    34501 tcataatccc gccaagccct gaaagcaggc cgctcatgcc ataaacaaac agcagcacca
    34561 gccagacctt aatgccggta agacgcgccg cctgcatatt gccaccgacg gcgtagatat
    34621 gaacgccaag cgtggtgcga cgcagaataa accagcacag cacgatcacc gccagcgcaa
    34681 tcaccaccag ccacgggacc ggcccgagat agttattgcc tatccactcg aaattaatgt
    34741 tggagttgat aaccgtggtg ccatcagcca gcagatacgc cgccccgcgc agcgccgtat
    34801 aggtgccaag cgtgacgata aacggcggca atccggcaaa ggccaccagc gcgccgttga
    34861 acagccccag cagcaggccg agcatcagcg ccgccgggat agacaacatg gcgaactccg
    34921 ggctaaggga gacaaccatt gccgccaccg ccgtggtgcc gaggattgaa cctacagaaa
    34981 gatcgatccc accggtcaaa atgatgaagg tcatccccgc cgccagcaca atgttgatcg
    35041 acgcctggcg ggtaatattt agcaggttgc tttcggtaaa aaagtttggc gcgataaagc
    35101 caaataccgc tacgatcagg atcaaaatgg gcaaaatgcc gaccgtttgc atcagatcgc
    35161 tcatcagcat ttttttggca gaggtttttg cagaggtcgt catgaggtca ccgcctggga
    35221 atgggcgtcg ttgacgccag tcgctaaggt cataatgttt tcctgggtga tgtttttgcc
    35281 atttagctca ccggcaatgc tgccttcgcg catcacatgg acgcgatcgc tcattcccac
    35341 tatctccggc agttcgctgg agatcatcag gatcgccacg cccttgcgcg ccatctcgtt
    35401 catgatccgg tagatctcac tttttgcgcc aacgtccacg ccgcgggtgg gttcatcgag
    35461 cagtaaaatg cgcgggccaa tcgccaccca gcgggagatc aacagttttt gctgattgcc
    35521 gccggaaagc ccgcccgcgc gtacctgggc atgaggcacg cgaatgttga gcaacttaat
    35581 ggcgtcatcg gaaatggttt gcgctttttt gcgattgagc attccccagc tggcatcgcg
    35641 ctccagggtt gccatagtga tgttttcgac tgccgccatt tcaaggaata agccttgttc
    35701 tttgcggttt tcggtgagaa aaccaatgcc aagatcgatg gcttcgcgcg gggagtggat
    35761 caccaccggt tcgccatcga cttcaatcat tccgcctgtt gctttccgca cgccgaaaat
    35821 taactgcgcc agttcggaac gtccggctcc caccagcccg gcgaggccaa caatttcgcc
    35881 ggaaagcacc tgcaggcagc tcggctggac tttgccgccg tcggtcaggt ggtgaacatt
    35941 caggcgggct ttaccgagcg ggatatcgcg ctctttattg aacagatcgc tcagtggcct
    36001 gccgaccatc atccgcacca gctccccggc attgaggtta tcgcgggtca ggctgccaac
    36061 gtattgcccg tcgcgcagca cgctgacgcg atcggaaagc tcatacactt ccgccatacg
    36121 gtggctgatg tagataatcg ccatcccttc atcgcgcagc cgcataatca gttcaaaaag
    36181 acggtgagtt tcacgggagg agagggcagc ggtgggttcg tccatcacca gaatgcggct
    36241 gttgcgatgt agcgcgcggg cgatttcgac ctgctgttgt tcggcaatgg tcagcgtcat
    36301 gaccttatcg ctggcgttaa actgcgcgcc gaggcggtcg atcactcttt gcgcctgcac
    36361 cagcatctct tttcgctgca ccagcccgcc gtgggaaagc tcgctgccga ggaaaatatt
    36421 ttctgccacc gtcaaattgg gtgccagctg catctcctga taaatcagcg taataccggc
    36481 gcttaaggca tcttttggcg tgcgaatgtg aaagggcttg ccgtctatca ggatctcgcc
    36541 gctggtggcg gtatacgccc ccgccagcac cttcatcagc gtgctttttc ccgcgccgtt
    36601 ttcccccatc agggcatgga tctcgccagg gtagaccgtc aaatccaccc ctttgagcgc
    36661 ataaaatttg ccgaaggctt tggcaatatt gcgcatctca agaaccggcg tgctgttcat
    36721 ggtggactcc tgcgaggttg tcgatatcag cactccatca caggaaagta aatgcaaaat
    36781 gtcagaagcc gttcatattt gtcatattgc gatttttctt cacggagcgt gatatggcgc
    36841 accccggacg tcactttttt gccagcgcgc gcgggcgatt gctgcttttg aatttgctgg
    36901 tggtggcggt gacgttgatg gtcagcggcg tggcggtaat gggctttcgt catgccagcc
    36961 agatgcaaga gctggtgcag cagcaaactg tagatgatat gaccggcagc ctgaatctgg
    37021 cgcgcgacac ggcaaacgtg gcaacggcgg cagtgcgatt gtcgcaggtg gtgggagcac
    37081 tggaatataa aggcgaagcc gagcggttgc aggagacaca gcgggcatta aaaagctcgc
    37141 ttgcacaact ggcgaacgcg cctttagcgc agcaggaagc cgggctggtg acgcgcatca
    37201 tcacgcgtag caacgaactg caaaccagcg tcggcggaat gctggaacgc gggcaaaggc
    37261 ggcatctgga gcgtaacgcg ctgttaagtt cgctgtatca aaacttaagt tatctgcggc
    37321 atctgcaaaa agtcactcac gcgcaagata atattttgct taacgagatg aaccgactga
    37381 ttgttgccgc cattgccacc cccgcgccgc aggcgattat tcatcaactg gtcggagtga
    37441 tgtctgcctt gccaactcac agcgatacgc cattggttaa tacgctactc gctgatttta
    37501 atcgtgaact gcgtaaactc gcgccacttt cggcggctct ggagcaaagc gatctggcaa
    37561 tcagctggta tatgtttcac atcaaagcgc tggtggcgat ccttaatggc gacatcaacc
    37621 gttacgccga acaggtggcg acgctctctg gtcagcgggt ggagcaaagt tacccggatt
    37681 tgcgctccgg cgagaggctg atcatgattt tcgccctgtt agcggtggtg atcaccgcgt
    37741 ttgcaggttg gtatatctat cgcaatctcg gctctaacct gacggcgatt tcccgcgcga
    37801 tgagccgcct ggcgcacggc gaagcggacg tcacttttcc ggcgttacag cggcgcgatg
    37861 agttgggtga actggcgcgc gcctttaacg tttttgcccg caataccgca tcacttgaac
    37921 gaacgtcacg gctgttgaaa gagaaaacca cgcagatgga gattgcccaa accgagcggc
    37981 aggggctgga agaggcgcta ttgcacagcc agaagttaaa agcggtcggg caactgacgg
    38041 gcgggctggc ccatgatttt aataatctgc tggcggtaat tattggtagc ctggaactgg
    38101 tgaacacgaa ctcacccgac gcgccgcgaa tacagcgggc gcagcaagcc gccgaacgtg
    38161 gcgcgctact gacccagcga ttgttggcat tttcccgcaa gcagtcctta catccgcaag
    38221 ccgttgaact aaaaacactg ctggaagagt taagcgaatt aatgcgccac tccttaccgg
    38281 cgacgctgac gattgagatt gaagcgcagt ctccggcgtg gtccgcgtgg attgacgtca
    38341 gccaactgga aaacgccatc atcaacctgg tgatgaacgc ccgcgacgcg atggaagggc
    38401 agacgggaat tatcaaaatt cgtacctgga atcagcgtgt tacccgcagc agtgggcagc
    38461 gtcaggacat ggtggcactg gaggtcatcg atcatggcag cggtatgtcg caggcggtga
    38521 aaagccaggt gttcgagccg ttctttacca ccaaacagac ggggagcggc agcgggctgg
    38581 gcttgtcgat ggtctatggt ttcgtgcgcc agtccggcgg gcgcgtggaa attgaaagcg
    38641 cgccggggca ggggacaacg gtcagattac agcttccccg cgcgctgacg gcggcgaaaa
    38701 acctatcgcc agcggcggta gaacagacag ctgttaatgg cgataaactg gtgttagtgc
    38761 tggaagatga agccgcggtg cgccagacca tctgcgagca gttgcacctg ctgggctatt
    38821 tgacactgga agcgagcagc ggcgaacagg cgctggacct gctggcggca tcagcagaaa
    38881 tcgatatctt tatcagcgac ttaatgctac ctggcggcat gagcggcgca gaagtggtca
    38941 atgcggcccg caaactttac ccgcacctga cgctgctact catcagcggt caggatttac
    39001 gccccagcca taaccccgcg ctgccggacg tggcgctgct gcgcaaaccc tttacccgcg
    39061 cgcagctggc gcaggtgctg gggcaaattg agggatgatt ttcagaattt atcaagaggc
    39121 tcgaagcaaa cgtgctgcta ttgtgagcaa ttctgttctg aattgaagtg atttacatat
    39181 gatcgtgatg ccaaccacct atagccctat gacggttgct cgttcttttc gcgtggtgga
    39241 agagatcgag ctttctggac gtctgttaca ggtaatttat gacaaccaga cgccaagagc
    39301 ggccatcatt gaagcggaca ttgaagtttt tgcaggcgtg ccgcgtcatc gggtacttgc
    39361 cgctctcgat ctgcagcgta aaaaagagtt cggcagcgat atcattgccg ttgaacgctg
    39421 gtgggaagac tcggatctgg tacaggtgga aggtatttgt gtctctccag gtgagcgcga
    39481 taagggtctg gcaactcggc tgtatgaagc gctaatcctg caatgcggta tcacgctaat
    39541 cagcgatttt gatcaatatg atggcgggaa gatgctctgg cagaagatcg cgcgcgagtc
    39601 cgatcagatt tccgtgtttg tattagacac tgaacaaggg gcattttggc cctacgatgg
    39661 cacgaaagtg atctacgatg gcggctgcat tccggaagag cgtatctgga gtatttcacc
    39721 ggatcaaaaa tgtcatggca ttgtattggt ggcggaaaat aaacagcgag ctaaccagct
    39781 aatggaagag tctgcgcctt accgaatttg agattactcc gctactccgg tcattcacac
    39841 tcaactaacg ttaagccagt ccatcgcaga gactggctta tgaaacctta ctccacgctt
    39901 tttcttttca ccctgctcac gctgtccacc gttccggccc aggccgatat tatcgacgat
    39961 actattggta atattcagca ggcgattaat gacgcttata actctgaccg tgggcgagac
    40021 tatgaagatt cgcgcgacga cggctggcag cgtgaagtga gcgacgatcg gcgcagacaa
    40081 tatgacgacc gccgccgcca gtttgaagat cgccgcaggc agctggacga tcgccagcgc
    40141 cagcttgatc aggagcgtcg gcagctagaa gatgaagagc ggagaatgga agatgagtat
    40201 gggcgatgag gttgggtatg gagcaggcat cgcccctcac cccaatgggg agagggagaa
    40261 aaccagcgca atattcaata tcgagcacaa tcgctcccct cgcccctctg gggagagggt
    40321 tagggtgagg gggccagcgc gcacattccc tctcatcacg ccaccccaat ctccatccca
    40381 tcaaaccccg cctcaaaccc cgacggcagt gcgttttcta tcagccacgc atcaaactgg
    40441 tggctgatat gggtcagaat cacccgtggc gagcggataa cctgattcag cgcaagcacg
    40501 gtatttaaat cacagtgatt acgcggcgca tccgcgcgcg gcgggtggct gcaatcaatc
    40561 accattacct gtggctgatt attgcgtaaa aatttcagcg ttttttccgg caggccagcg
    40621 gtgtcagata gccacgccac ccggctatgt gccgtttcca gcagataacc gaaggtcagt
    40681 tttgagtggt taagcggcag cggcgtgacc tgcaacccct gcaaatcaaa caccacaaac
    40741 ggttccactg tgtggctgaa atcaagcagg cccggatgtt taaacagatc gtcgcaaccc
    40801 tggtcatccg gcgggccgta gatcgggatc ggatcgccca cgccccagcg cagcggaaac
    40861 agcccctggg cgtgatccat atgataatgc gtcagcaaaa actgctggaa cgatccgggc
    40921 gaccagcgat cggcgagatc gtgcagcccg gcgtcgatca gggtgattgc gtcgttaaac
    40981 ttcactacgc cgctgcatgg ctggcggcga tactgcggtg agcgccgcgc tcggtcgcag
    41041 gccgcgcact cgcagcccca tgccggaacg ccctgtgcgc cgccagtgcc ggtgagcgtg
    41101 agggtcaggc tcatgttaca gcaccttggt gaagcggaag tgactctgct cgtagccttc
    41161 tcgcagatag aaacggtgcg cgtcgtggcg tttcacgttg gtcgaaagcg cggtcatttc
    41221 ggccccggcc tggcgggctt cttcttctgc ccatgccagt aatttactgc cgacgttcag
    41281 accgcgcgcc tgcggcatca ccaccagttc ctgaatttcg ccgatccagt tgacatgatg
    41341 cagatgaaac tgcaaatgca ggccgatcat gctgacaact tcgccatcca gcagcgccag
    41401 atggtagcgc atgtttgggt cgcgcaggtt ggcgttaaag cccacgcgaa acgcatggtg
    41461 gtcaaactcc gcctgtttta gctcacaaat cagcgcgtaa actgcgtcgg tgtcgtactg
    41521 cgtagccggg cgaagctcac aagcaggcat ggtgtttctc cttctgatga atcagcgtca
    41581 gcagcttgtc gaccgactgg cgcaggctgc cgtcattatt gagcgtaaga caatcctgag
    41641 gagtatagcg ggcagcgcgt gctagtcggg cgttaatttc actggcattt tcacgaccac
    41701 ggttttcgag gcgctggcgc aggatctccg gcgaaacctg taagcaaatg ggcagcagcg
    41761 ccgattgata gcgcgcccgc gcctgcggca gatgggcgcg tgagccgttg accagcacgt
    41821 cgaatccggc gtgcagccag agatcaatct caacgccgac gccataatac agactgttag
    41881 cgtgccagct taaggccaac agattttgcc ccgcgcgggt aaaaaactcc tgctcgctca
    41941 gggcgatatg gttttcactt ccggcgctgg cgtcgcgcgt aatgtagcga tgcgccacca
    42001 gtaactgagt ttgttcccgc aggctgattt ccgccagcag gctgtctttc ccggagccgg
    42061 acggccccat taaccaaatc agttttccca tcatcagaac acccttttac cctgacgcca
    42121 gacgtggtcg atatgaatat gattgtcctt gcgatgcgcc agcaccaggt ccgcgcgttt
    42181 gccctcgcca atcaccccgc gatcctgaag attaagcgcc tgcgccggat tttgggtcac
    42241 taacttcacc gcctgcggca gcgtaaagcg gttgctctgg tcatcggcga cgcgaaatgc
    42301 cgcatccagc aggctggcgg ggtagtagtc ggaagagagg atatccagca ggccaagctg
    42361 cgccagttca ctggccgcca cgttgccgga gtgcgaaccg ccgcgcacga tattcggcgc
    42421 gcccatcagt acgttcatgc catgcttgcg cgaggcttcc gccgcttcaa acgtggtggg
    42481 aaattcggcg atcacgctgc caagcaggtg ggattcggca acgtgggcgt gggtggcgtc
    42541 atcatggctg gcgagcgcaa tttgtcgcgc gcggcacagg gcggcgatgg attcgcgatt
    42601 cggctgcgac cagcgtgcgg caagcgccag ttgctcttct tcgtactgct gcatctgcgc
    42661 atcggtgagc gagtatttgc cctgataata ttcgcgatac ttctcgcggt tggcgaactg
    42721 gcgctggccc ggcgagtggt ccatcagcga caccagcgtc accggctcgc gctgcaccag
    42781 tttttcaaac agcggcagcg tggtgtgatg cggcagttcg cagcgcaggt gcagacggtg
    42841 ctcggcgcgg atgtcgccgt aatgacgggc cccggtcgcc atcagccgct cgccgatatt
    42901 gccgcctgcc gagacctggc gacgcaggcc gtcgagtgga tgctgatgca ccacgcccca
    42961 ttcggtgcgc agcaggcgac ggcggtcggc ctcgctcatc gcatacaacg aacgattctc
    43021 gtagcgaatc tccccctgct gcggcgtcag gcgcgcggag attgatttca gcagcgtggt
    43081 cttcccggag ccggattccc cgacgatgcc cagcacttcc cccggccata aatcaaaaga
    43141 gacatcgcta aagcctttgc ccggcgcgta aaggtgggtc aggttattga ccgaaagtaa
    43201 cggttgattc attggttttt tgcctcgctc tgttggcggc aataatcggt atcggagcag
    43261 acaaacatgc ggtttccggc gtcatccagc accacttcgt cgagatagct gtgggtcgat
    43321 ccgcagatgg cgcatggctc atcccactgc tgaacggtga acgggtgatc gtcgaaatcg
    43381 agactttcca cgcgggtaaa cggcggcacc gcatagatgc gcttctcgcg ccctgcgccg
    43441 aacagctgca gggcgggcat catgtccatt ttcgggttat cgaatttcgg gatcggcgac
    43501 gggtccatca cgtagcgccc gttcaccttc accggataag cataggtggt ggcgatatga
    43561 ccgaagcggg cgatatcttc atacagtttc acctgcatca cgccgtactc ctccagcgcg
    43621 tgcatagtgc gggtttccgt ttcgcgcggc tcgataaagc gcagcggttc ggggatcggc
    43681 acctggaaaa taactatctg atcttcggtc agcggcgttt cggggatgcg atggcgcgtc
    43741 tggataagcg tcgcatcgtc cgtacgttca gtggtgttca ccccggtcac gcgcttgaag
    43801 aagttgcgaa tcgacacggc gttagtggtg tcgtctgcgc cctggtcaat caccttcaac
    43861 acgtcgcttt cgccaatcac gctggcggtg agctgaatgc cgccggttcc ccagccgtag
    43921 ggcatcggca tctcgcgccc gccaaacggc acctgataac cggggatagc caccgctttt
    43981 aagatggcgc ggcggatcat gcgtttggtc tgctcgtcga ggtaggcaaa gttgtagccg
    44041 ctcagattag ccatggttct gctcctgttg cagacgtttg agtagctcca gttcggcctg
    44101 gaaatcgacg tagtggggga gtttgaggtg cgagacaaag cctgcggctt cgacgttgtc
    44161 ggcatgcgcc agcacgaact cttcatcctg cgccgggcct gtcgcgtgct cgccgtattc
    44221 cggggcttgc aacgcacggt caaccagcgc cattgccatc gctttgcgct cgctcatgcc
    44281 gaataccagc ccgtagccgc gcgtgaagtg cggcggctca tccggcgggt cgataaaacc
    44341 gttgaccatt tcacactcgg tcatcagcag ttcgccgacg tttaccgcaa atcccagctc
    44401 ttccggcaca atcgacacgt cgatgtaacc actgcggatc tcgcccgcga acgggtgatt
    44461 gcgtccgtaa ccacgctggg tggagtaggc cagcgccagc aaatagcctt cgtccccgcg
    44521 cattaattgt tgcaggcggg aggagcgtga gcaggggtaa accggcggcg tgcgggtgat
    44581 gtcatccggc tgtgcgccgc tatcttcttc aaacttcgcc agcccctgac gcgccagcag
    44641 gctgaaaacg tgcggcgacg gctgttgttc gctgtcggcg gtggtcagcg tcggcgcttc
    44701 accgtttgcc agcagggtga aatcgagcag gcgatgggtg tagtcgtagg ttgggccaag
    44761 cagctggccg ccgggaatgt ctttataaac cgccgaaata cgccgttcga gacgcatctc
    44821 ggtggtgtcg agcggctcgc ttaccgccag tttcgccagc gtggtgcggt aggcgcgcag
    44881 caggaaaatt gcttcaacgt tatcgccgct ggcctgtttc agcgccagcg ccgccagttc
    44941 gcggtcggca acgccgcctt cggtcatcac gcgatccacc gcgagattaa gctgctgttc
    45001 aatttgggcg acgctcagtt cgggcaaatc ggtatcgccc cggcgtcggc tctcttgcag
    45061 ggcgtgggcg gcgtcgatcg ccttttcgcc ccctttcacg gcaacgtaca tcagcacacc
    45121 tccacatgag tggttcgcgg aatagccagc aggcgttcgc cgcaggtcag gatcaggtcg
    45181 atgccgagcg ggaacgggtg cgggcgctcg gtgagttcgt gcagaatgca ctccggcagc
    45241 tgcggagcga tcattcgttc ttcggcaata cccgcgccgg tgagacgcaa catgcgcccg
    45301 ccgctcaggc tggcgacctg taaaatcagc gtcgcgcccg cttccggcgc aacggcggtg
    45361 ccggtggaaa gggctttgag ctgtttgctg gaaattccgt catccgtcac cgcgaaggtc
    45421 gcctgttccg gctggctgac cagcggcgcg ttggtatgaa aacgcaggct ctgattgacg
    45481 atatcgttac tcaatggcgc ggcaagccac accggcgtgt cgttatcagc cagcgtcagc
    45541 agcacgctgg tggtggcgat attcagcggc tgccagccgc gcttgagctg atgcagggcg
    45601 acaatcacgc ccggctcgct tatggccttt aacaggcgac gaaaactgtg ctgggcatcc
    45661 tgcacgggaa gcataaaagc ggtttccagg gtcatgcgtt gtctccgcga accatcgtaa
    45721 agaagtcgac ccggctggcg ttcacttcgg cctggcgtgc ggcaatgcgt gccatacggt
    45781 cagcgtccag cggggcaata agggtttctg ataagttttg aaagtggcga gattgctgca
    45841 tcagcgcatc aatcagcgcg cagcgttcgg tgtgctgttt atcacgcccc agcacccagc
    45901 tgtagccgag ggtgccgtca gtcaggcgta cggcggcgcg ggtcagcgtg gcgtcgccgg
    45961 caaaaaagcg ttcgccggtg ccgcccattc gcgcctgaat ctgtaccagg ccggtttcgg
    46021 cggtgcggat cacttcatag tcggcggtga tgttcagcgc gttcaggcgg gctgccagtt
    46081 cagccggttg gctgtgcgcc agtacggaca tccagtgctg gcgggtcgcg gtatctgcgt
    46141 gcattcagtg ctccatagtg aattcaatca tgtcggcgcg cgtcaggctg acggagtact
    46201 ccgccgggct gctttcaccg tcacggtggt taagggtgcg cacgcacagc agcggcgaca
    46261 tattcgggat ttcaaggcgc tggcactctt tggcctgggc gcggcgggcg ctgatccgcg
    46321 tctggctgcg gcgcagcgca attccggttt gctcgcgcag aaaatcgtgc agcgaaccgc
    46381 tgtcgaagcg ttgcagcgtc ggccaaaggg tgaggtccgc aaagtagtgg tcgattaaac
    46441 agagcgcgac gccgttgacc cggcgcaggg tgcgcaaatg gataacgttc tcgccctcgg
    46501 taatccccag cgcgtcagca acgtgaccgg acgcggggcg caataccgac agcagttttt
    46561 cgctggtggg atggctgccc tggtccagca ggttctggct aaaacgcgcc tgggcgttga
    46621 gcgggtaatc gaacgggcgc atcagcacca gcacgccgac gccctgacgg cgctgtaccc
    46681 agcctttttc caccagttgg tcgatggcgc ggcgcagggt gtggcggttc acctcaaagc
    46741 gcgccgccag ttgctgctcg gcgggaagat agtcgccgca gcggtagtgt tgacgaagct
    46801 cctgctcaag ttttgcggct atctcctgat agcgtgttgg gtagctggtc ggatgtgtag
    46861 acaagtgcat agatatcaat gcctcgctta tcagataaag tgcttacgca accgttgaga
    46921 gaggaaatcc agcaggctga ccgtgacgat gataagcacc atcagggcgc aggtttgctg
    46981 gaactggaaa ccgcgaatcg cttcccacag ggtgacgccg atcccgcccg cgccgaccat
    47041 gccgacgacg gtcgccgagc ggacgttgga ttcgaagcga tagagggagt aggagatcag
    47101 cagcggcatc acctgcggca gcacgccgta aaggatctct tcgagtttgt tggcgccggt
    47161 ggcgcgaatg ccttccaccg ggccgggttc gatcgcttcc accgcttcgg aaagcagctt
    47221 ggagagcacg ccggtggtgt ggataaacag cgccagcacg ccagctaacg ggccgaggcc
    47281 gacggcgacc acgaacagca tggcgaagac catttcgtta atggcgcggc aggcgtccat
    47341 caggcggcga acgggctggt aaacccacca cggcaccaag ttttcggcgc tcatcagacc
    47401 gaaggggatg gagagaacca ctgccagcgc ggtgccccag acggcgattt gcagcgtgac
    47461 ggccatttcg gtgaggtaat cctgccactg gctgaaatcg ggcgggaaga agtcggcagc
    47521 gaaggttgcc atgttgccgc cgtctttaat cagcgtaagc ggggccattt ccgcgccctg
    47581 ccacgagacg accagcacgg cgagaacaac ggcccagctc agaagcgaga accagctacg
    47641 cttgggtggg gcgatggtga tggtttgcat gtttggctcc ggtctgtagg ccggataagg
    47701 tgcttgcacc gcatccggca tcaacgcctg cacattgtcg gatgcgacgc cggggcgtct
    47761 tatccgacct acgatttact gcaccgcttt actcaccgaa ctcatcgcgc ttaacgcgtt
    47821 gttcaggcgg tccaggtcat ccagctgcgc ctgaatctcg gtggttttcg ccagcttgtc
    47881 ctgctcattc agccctttat tgcctttcac gctttgtatc tctttaaaca gtgcgagctg
    47941 gcgaatcggc accagttgca ggtcgctgga agcgcggaac ggcgcccagc ccaggcgttc
    48001 cagcaccgct ttctcttccg gcgtttttcc gtagttcata aagaagtcgt agatcttgtc
    48061 tttggtagtt tcggacagat tcttgcgcca gacgatcgga tcgcctagga tcagcggcga
    48121 tttccagatc accttcagct ctttaagctt ctccggcgcg gaggttttca gcttgtcgag
    48181 gttttcggtg ttgttggtgg caacatccac ctgcttgttg gcgacggcca gcgcgttggt
    48241 ttcatgcccg gcgttgacgg tgcgcttgaa gtcgctggcg gagatattgt ttttagcgaa
    48301 gacgtagtaa ccggggacga ggaagccaga ggtggagtta ggatcgccat tgccgaaggt
    48361 gagatctttc cgcttcgcca gcagatcgtt caggttgttg atcgggctgt ctttgttgac
    48421 gatcaacacg ctccagtaac ccggcgatcc atccgccgcg accgtctggg cgaagacctg
    48481 gccattggcg cgatccaccg cttccatcgc cgacaggttg ccgtaccagg caatatccac
    48541 tttattgaag cgcatcccct ggataatgcc cgcgtagtcc ggggcaaaga aggcgttgac
    48601 cttcacgccc agtttcttct ccatatcctg taaaaacggc gtccattgcg gtttcaggtt
    48661 ttgctgtgat tccgttgaaa taatgccgaa attcagcgcc ttttcctgct cttcggcgtg
    48721 tgccggggtt aacagggtgc tgaggctgaa catgctggtg aaggccagcg aggcaattat
    48781 cttagcgttc attcgttttc ctcaatgatg gggatatcag gcagctttcg cgttctcttc
    48841 gatgcggtta atgctgcggt agagatggtc aaaacgttcg ttatcaaaca gttggctgct
    48901 gccgtcgtag aagacgtgcc cctgacgcag ggcgacgatg cgttcgcagt agcgcagggc
    48961 gtaatccacc tgatgcagcg tgacgaccac ggtgatgccg tcgttctggt tgatgtcgcg
    49021 cagggtgtcc atcacgatgc gcgctgattc cgggtccagc gaggcgatgg gttcatcggc
    49081 cagaatcact ttcgcctgct gcatcagcgc acgggcaatc gccacacgtt gctgctggcc
    49141 gccggagagg gtggaaacgc gctgatgggc aaaatgcacc atgccaacgc gggtcagcgc
    49201 ctgtaacgcg cgctgtttct gctcgccggt gaaccagcta aaacaggtgc gccagaacgg
    49261 cgtgctgccg agcgcgccaa tcagcacgtt ctccagtacg ctcaggcggt tcaccaggtt
    49321 gaattgttga aagatgtagc cggtgttggc gcggcttttg cggatatcgc gggccaggcg
    49381 gccttcgcgc tggacagtgc ggcccagtag ctcgatatgg ctaccggcgg atttatcgcc
    49441 ggtaatcaaa ccgcttaaat gacgtaaaag ggtggatttg ccggaacccg acggcccagg
    49501 cagagccacc atttcactgt gatgaatgtt cagatcaacc gcatgcagcg cttgatgctg
    49561 attgaaggtt ttggcgagct tctcgacacg gataatcgtt tgcatgatgc agcctcccta
    49621 aaaaagtggc tccatcgtgg cggattattg tgacgatttg gttaattaaa agtgactaac
    49681 agatgaagag ttaacgggaa ttcgatgaca gtgcgggagg gcgggcctcc cggcgggtta
    49741 ttgcgttggt tgttgtttga cgacattaat catccacggt acgccaaatt tatcggtgac
    49801 tttgccaaag ccatgcgccc agaaagtttc ctgccaggcc atttcgattt ttccgttagc
    49861 ggcaaggtta tcaaaccagc gttttccttc ttcgacctgt tgcgaatcga gcaccagcgt
    49921 aaagccggag tagctggctt ttcctgacgg tatagcatcg ctcatcatga tgtcgcttcc
    49981 ggcaatgcgc acgttggcat gagtgatggc ggtatcggga aattgcattc cggaagggca
    50041 gttctcggcg ctgtcctgcg ctgatttcgg catttcgccg aagctgattt tatagagcag
    50101 ttccgcgccc aacgtacgtt gataataggc aatcgcgtcg gaacagttac cggcaaaaga
    50161 gaggtaggga cttaacggca tgatggtgac ctcagttaag agaagccagt taagtgtagt
    50221 tcgaaattta tacagatgag aggctgcctg ataagacgcg caagcgtcgc atcaggcagt
    50281 cggcactgtt gccggatgcg gcgtaaacgc cttatccggc ctacggaggg tgcgggaatt
    50341 tgtaggcctg ataagacgcg caagcgtcgc atcaggcagt cggcatcgtt gccggatgcg
    50401 gcgcgagcgc cttatccggc ttatcgatca caatacaatc agttcttttt cacaaactca
    50461 gatttcagtt tcatcggacc aaaaccgtcg attttgcaat cgatgttatg gtcgccttca
    50521 accaggcgga tgtttttcac tttggtgcca attttcagca tcgaagagct acctttcacc
    50581 ttcagatctt taatgatggt aacgctgtcg ccgtcagcca gcagattgcc gttagcatct
    50641 ttaacgatca gctcgtcgct ttcctgtgca ggttctgcgt cgttccattc gtaggcacat
    50701 tccgggcaga tgtacatgcc gttatcttcg taagtgtatt cggagttgca ttttgggcag
    50761 tgtggtaatg acatgtggat ttcctcaaaa gtcagcaggg caaaagcgcc aaaaaacggc
    50821 agattgccga aaaaggccgc aattatacac aaaatccctg agtttgtcgg gggtactgct
    50881 gattttagac gacgatcgtt ttcatttgaa aggaaacgca aaaagaaaat aatctttttg
    50941 cctctattta ttatttaaga taatgttaat gctctacttg ttcttttttt tattcacatg
    51001 atatttttat ctttctgacg ttttaaacgc atcagaagac gttatttatt ctgataaaaa
    51061 aaacacacta aatataaaat tatttcttgc agtaattata aaagccgaaa acagaactca
    51121 aaaacaatct ggctaaataa aagtaacaaa atttgcttta aggaa
//
LOCUS       DBKEKI010000013        49229 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.013, whole genome shotgun sequence.
ACCESSION   DBKEKI010000013 DBKEKI010000000
VERSION     DBKEKI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.013"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            97..249
                     /gene="hokA"
                     /locus_tag="ACUQPX_000724"
     CDS             97..249
                     /gene="hokA"
                     /locus_tag="ACUQPX_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HHV9055513.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(226..297)
                     /gene="ysaE"
                     /locus_tag="ACUQPX_000725"
     CDS             complement(226..297)
                     /gene="ysaE"
                     /locus_tag="ACUQPX_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HHV9055514.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFAG"
     gene            complement(436..648)
                     /gene="cspE"
                     /locus_tag="ACUQPX_000726"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(436..648)
                     /gene="cspE"
                     /locus_tag="ACUQPX_000726"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HHV9055515.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(929..1219)
                     /locus_tag="ACUQPX_000727"
     CDS             complement(929..1219)
                     /locus_tag="ACUQPX_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HHV9055516.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1653..2363
                     /locus_tag="ACUQPX_000728"
     CDS             1653..2363
                     /locus_tag="ACUQPX_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HHV9055517.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2413..3387)
                     /gene="ghrB"
                     /locus_tag="ACUQPX_000729"
     CDS             complement(2413..3387)
                     /gene="ghrB"
                     /locus_tag="ACUQPX_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HHV9055518.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3491..4150)
                     /locus_tag="ACUQPX_000730"
     CDS             complement(3491..4150)
                     /locus_tag="ACUQPX_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HHV9055519.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4303..6636
                     /gene="bisC"
                     /locus_tag="ACUQPX_000731"
     CDS             4303..6636
                     /gene="bisC"
                     /locus_tag="ACUQPX_000731"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HHV9055520.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPRHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6605..7045)
                     /locus_tag="ACUQPX_000732"
     CDS             complement(6605..7045)
                     /locus_tag="ACUQPX_000732"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HHV9055521.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7042..7605)
                     /gene="tag"
                     /locus_tag="ACUQPX_000733"
     CDS             complement(7042..7605)
                     /gene="tag"
                     /locus_tag="ACUQPX_000733"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HHV9055522.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7763..8461
                     /locus_tag="ACUQPX_000734"
     CDS             7763..8461
                     /locus_tag="ACUQPX_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HHV9055523.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8690..9898
                     /locus_tag="ACUQPX_000735"
     CDS             8690..9898
                     /locus_tag="ACUQPX_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate MFS transporter"
                     /protein_id="HHV9055524.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10222..11913
                     /gene="eptB"
                     /locus_tag="ACUQPX_000736"
     CDS             10222..11913
                     /gene="eptB"
                     /locus_tag="ACUQPX_000736"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HHV9055525.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12005..12081
                     /locus_tag="ACUQPX_000737"
     tRNA            12005..12081
                     /locus_tag="ACUQPX_000737"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12039..12041,aa:Pro,seq:cgg)
     gene            12825..14432
                     /gene="dppA"
                     /locus_tag="ACUQPX_000738"
     CDS             12825..14432
                     /gene="dppA"
                     /locus_tag="ACUQPX_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HHV9055526.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVSPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14740..15759
                     /gene="dppB"
                     /locus_tag="ACUQPX_000739"
     CDS             14740..15759
                     /gene="dppB"
                     /locus_tag="ACUQPX_000739"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HHV9055527.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15769..16671
                     /gene="dppC"
                     /locus_tag="ACUQPX_000740"
     CDS             15769..16671
                     /gene="dppC"
                     /locus_tag="ACUQPX_000740"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HHV9055528.1"
                     /translation="MSQVTENKVISAPVPMAPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16682..17665
                     /gene="dppD"
                     /locus_tag="ACUQPX_000741"
     CDS             16682..17665
                     /gene="dppD"
                     /locus_tag="ACUQPX_000741"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HHV9055529.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCYYPLDDAGRPTL"
     gene            17662..18666
                     /gene="dppF"
                     /locus_tag="ACUQPX_000742"
     CDS             17662..18666
                     /gene="dppF"
                     /locus_tag="ACUQPX_000742"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HHV9055530.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18696..19967)
                     /locus_tag="ACUQPX_000743"
     CDS             complement(18696..19967)
                     /locus_tag="ACUQPX_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HHV9055531.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20443..20550
                     /locus_tag="ACUQPX_000744"
     CDS             20443..20550
                     /locus_tag="ACUQPX_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HHV9055532.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(20637..22316)
                     /gene="bcsG"
                     /locus_tag="ACUQPX_000745"
     CDS             complement(20637..22316)
                     /gene="bcsG"
                     /locus_tag="ACUQPX_000745"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HHV9055533.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(22313..22504)
                     /gene="bcsF"
                     /locus_tag="ACUQPX_000746"
     CDS             complement(22313..22504)
                     /gene="bcsF"
                     /locus_tag="ACUQPX_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HHV9055534.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(22501..24072)
                     /gene="bcsE"
                     /locus_tag="ACUQPX_000747"
     CDS             complement(22501..24072)
                     /gene="bcsE"
                     /locus_tag="ACUQPX_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HHV9055535.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQAQTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            24345..24534
                     /gene="bcsR"
                     /locus_tag="ACUQPX_000748"
                     /pseudo
     CDS             24345..24534
                     /gene="bcsR"
                     /locus_tag="ACUQPX_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
     gene            24546..25298
                     /gene="bcsQ"
                     /locus_tag="ACUQPX_000749"
     CDS             24546..25298
                     /gene="bcsQ"
                     /locus_tag="ACUQPX_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HHV9055536.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            25295..27903
                     /gene="bcsA"
                     /locus_tag="ACUQPX_000750"
                     /pseudo
     CDS             25295..27903
                     /gene="bcsA"
                     /locus_tag="ACUQPX_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            27914..30253
                     /gene="bcsB"
                     /locus_tag="ACUQPX_000751"
     CDS             27914..30253
                     /gene="bcsB"
                     /locus_tag="ACUQPX_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HHV9055537.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLSTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            30260..31366
                     /gene="bcsZ"
                     /locus_tag="ACUQPX_000752"
     CDS             30260..31366
                     /gene="bcsZ"
                     /locus_tag="ACUQPX_000752"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HHV9055538.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTGPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            31348..34821
                     /gene="bcsC"
                     /locus_tag="ACUQPX_000753"
                     /pseudo
     CDS             31348..34821
                     /gene="bcsC"
                     /locus_tag="ACUQPX_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            34903..36891
                     /gene="hmsP"
                     /locus_tag="ACUQPX_000754"
     CDS             34903..36891
                     /gene="hmsP"
                     /locus_tag="ACUQPX_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HHV9055539.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            37075..38361
                     /gene="dctA"
                     /locus_tag="ACUQPX_000755"
     CDS             37075..38361
                     /gene="dctA"
                     /locus_tag="ACUQPX_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HHV9055540.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVEVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            38582..40078
                     /locus_tag="ACUQPX_000756"
     CDS             38582..40078
                     /locus_tag="ACUQPX_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HHV9055541.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(40174..41103)
                     /gene="kdgK"
                     /locus_tag="ACUQPX_000757"
     CDS             complement(40174..41103)
                     /gene="kdgK"
                     /locus_tag="ACUQPX_000757"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HHV9055542.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLHECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            41335..42102
                     /gene="pdeH"
                     /locus_tag="ACUQPX_000758"
     CDS             41335..42102
                     /gene="pdeH"
                     /locus_tag="ACUQPX_000758"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HHV9055543.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQLLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            42172..44232
                     /locus_tag="ACUQPX_000759"
     CDS             42172..44232
                     /locus_tag="ACUQPX_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HHV9055544.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLTPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKQRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVIGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(44414..45736)
                     /locus_tag="ACUQPX_000760"
     CDS             complement(44414..45736)
                     /locus_tag="ACUQPX_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9055545.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIATWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(46147..47160)
                     /gene="yhjD"
                     /locus_tag="ACUQPX_000761"
     CDS             complement(46147..47160)
                     /gene="yhjD"
                     /locus_tag="ACUQPX_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HHV9055546.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(47209..48108)
                     /locus_tag="ACUQPX_000762"
     CDS             complement(47209..48108)
                     /locus_tag="ACUQPX_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHV9055547.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            48628..>49229
                     /locus_tag="ACUQPX_000763"
     CDS             48628..>49229
                     /locus_tag="ACUQPX_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHV9055548.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALVVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT        11735 a        12331 c        13324 g        11839 t
ORIGIN      
        1 caactttttg gggtcagtac aaagtgaggc taccctatgc ctgaacacca taaggttagc
       61 ctcttactcg ttggaaatca acacaggggg ctgggaatgc cgcagaaata tagattactt
      121 tctttaatag tgatttgttt cacgctttta tttttcacct ggatgataag agattcactg
      181 tgtgaattgc atattaaaca ggagagttat gagctggcgg cgtttttagc ctgcaaattg
      241 aaagagtaag agtcttcggc gggaaattat tcccgcctta cttacggcgt tgcgcattct
      301 cattgcaccc acatttattc ttcacaaaaa taataataga ttttattacg cgatcgatta
      361 tttatttcct gaaaaaaaat aaaaaaatcc ccgccaaatg gcagggatct tagattctgt
      421 gcttttaagc agagattaca ggctggttac gttaccagct gccgggcctt tagcgccgct
      481 ttcgatggtg aaggacactt tctgaccttc gtccagagat ttgtaaccat cgttctggat
      541 agcagagaag tgtacgaaca catctttaga gccatcgtca ggagtgatga agccgaagcc
      601 tttgtcagcg ttgaaccatt ttacgatacc agtcatttta ccggacatag tgtattacct
      661 ttaataatta agtgtgcctt tcggcgatat ggcgtgcttt acagattttg aagcgttaaa
      721 ggaatgtgca ctacgagggg tatcaacgat aactcttgaa gggacttgcc ttactacact
      781 gctttaatgg tctgtacgtc aaaccgttga tgtgcattaa gccacgcatt ggcgggtgat
      841 gcaacaatta tttttcatat ttatgattaa tcggttggaa aacggtcctg tcatcaggac
      901 cgtaaacagc aataaagtgg ataaaagtct atttcatcaa ctgcttactt aatgccgggt
      961 tggcttgaat caaacgcatc aattttagtt cggcacttga aggcttcacg cgtctggatt
     1021 cccattcctt taccatggcg actgatacgc ccaaaacccg ggcgaaatca tcgattttta
     1081 atcctgtccc ttttcgtaac tgctcaattt cggtacagga cgttgttctg tgcgtcaggg
     1141 taattttctg cgtttcatct ttaaaaacaa tctgttccag gctgctcaac agctcatgca
     1201 ttggatcttt atattccatt gaaaactcct caaatcacac tgcgggatcg tgaattgcat
     1261 cgaagctcat taagaatagt cgggaaaagc agacacaacg gggtgaagtc agggcatgct
     1321 ctggctatga ttcgattcag cctgtcattt agcgtgactt atagccacct aacgatctga
     1381 taaatgccga tatatctctt atcactcctg ttttgtaaag atttcgcaaa tctaaccttc
     1441 tgcgaaggcg taaatctctg gctacaaaaa aggcaacggc actatcattt tttaacaatg
     1501 aaacaacaaa ttaaccttgt ttcattttct ccatgatttt accaggaaga ttacgtagaa
     1561 agggtatcct gcaagcctgt cctggactat ccttgtcacg tcagacacgc gtgtgtcgtt
     1621 gtgcgctttt tttgggtgaa aggagtaaga aaatggcgac aggaaagtcc tgctctcgct
     1681 ggtttgcgcc tcttgcggcg ttattaatgg tagttagcct gagtgggtgt tttgataaag
     1741 aaggcgatca gcgtaaagcg tttatcgact tcctgcagaa tacagtgatg cgtagcggtg
     1801 aacgtctacc aaccctgact gccgatcaga aaaaacagtt tggtcctttt gtctctgatt
     1861 acgcgattct gtatggttat tctcagcagg tgaatcaggc gatggattcc ggtctgcgcc
     1921 cggtcgtaga cagcgttaac gcaattcgcg taccgcagga ttacgttacg caaagcggtc
     1981 cgctgcgtga aatgaatggt tcgctgggtg tgctggcgca acaactgcaa aatgcgaagt
     2041 tgcaggccga tgctgcgcac tctgcgctaa aacagagcga tgacctgaaa ccggtatttg
     2101 atcaggcatt caccaaagtg gtaacgacgc cagctgatgc attgcaaccg ttgattccgg
     2161 cggcacaaac tttcacgcaa caactggtga tggttggaga ctacattgct cagcaaggta
     2221 ctcaggtgag ctttgtcgca aatggcatcc agttcccgac ttcacagcag gcaagtgaat
     2281 ataacaaact gattgcgcca ttaccggcac aacatcaggc gtttaatcag gcctggacta
     2341 cagcagttac tgcaacccaa taaagagtaa aagcccgagc gattatcggg ctttacttca
     2401 cgcagtcgcg gcttagtccg cgacgtgagg attcacacag ttcttctcaa cctttccttg
     2461 taacgcatca atcaaattat ccacggcaca ggcggccatg ccataacgcg tctcatgggt
     2521 ggcagatcca atatgcggta ctgcgacgac gttggccatt gagagcaacg gcgaatctac
     2581 ggacagtggc tcttgttcga agacatccag cccggcggcg tgaatttcgc ctttctgcaa
     2641 tgctgcgatc agtgcatttt cgtcaaccac cgggccacgt ccggcattaa tgaaaatggc
     2701 ggaggatttc attttggcga attgttctgc gccaaacaga tgatgcgtct catcagttaa
     2761 cggcaggatc aggcaaacga aatctgactc ttgtaacaga gtatccaaat cgcagtagcg
     2821 ggcgttgaag cgttcttctg cttctttatg gtggcggcgc gcgttataga ggatgggcat
     2881 gttgaagcca aagtgcgcac gttgtgccag cgccatgccg atccgtccca tcccgacaat
     2941 gcccagtgtt ttatggtgaa cgtcagtgcc gtaccagtcc gggcctatgc tcgcggtcca
     3001 ttcgcctgct tttacccgtt ctgctacctc cacaacccga cgagcggtag acaacaccag
     3061 cgccatcagc gtatcggcga cggtttctgt taatacggtt ggcgtgtgca tcagcagaat
     3121 ttttcgggcg gtaagcgcat cgacatcaaa attgtcatag ccgacggaga tcgttgatgt
     3181 ggcacgcagt ttcggcattt tttccagcaa tgcggcatct acattctcgt ttgaacccag
     3241 taaaccttca gcttctgcaa aaattgctgc attttgaacg acggtttgtg ggctgaggtt
     3301 tgccacctgg tgaacggtga aatgcgcttg caggcgttgc agtaaatcat caggtaaggc
     3361 tttgtagagg ataacggacg gcttcatgct tctctccatt cactgataag gcgatcacct
     3421 tagcctgaaa tatgatgaag gaaaactgaa gaatgccagg tgatttctgc atcacctggc
     3481 atgaaaggga ttacagcggg cttaaggtaa tttctacacg gcggttttgc gccttacctt
     3541 ctgcggtgct gttgctggcg attgggttag ccgggccaag gccctgagta cggatgcggc
     3601 tggcgtccac gccctgggtg atcaacgcgc tggcaacgga atccgcacgt tgctgggaga
     3661 gacgcatgtt caggtcgtga ccccccgtgc tgtcggtata accaatcacg ttaaccgccg
     3721 ttttcggata ctctttcagc accattgcca cgccggtcag ggtgttagcg cctgccggtt
     3781 tcaggggcgc gctgctgctg tcgaaggtca cattgttcgg catattgagg ataatgttat
     3841 ccccgctgcg ggttacgcta acaccagtgc cgcgcatttt gtcacgcagc ttcgcttcct
     3901 gcacatccat gtaataaccc acgccgccgc ccagagctgc gcctgctgct gcgccaatca
     3961 gcgcgccttt accgcgatct ttcttcgaag aagagagcgc accaataccc gcgcccacga
     4021 gagagcccag acctgcgccg atagcagatt tgcctgcttc gcgttcgccg gtgtaagggt
     4081 tagttgtgca gccagatacc gccagagcgc cactcactac ggcggcaata agataaacac
     4141 gtttcttcat tgttaatcct taataacctt tttattcttt gccacgggtt ccgtggcggg
     4201 agattatgcc gcgtgaacat gaagattatt cctgggaata ctcggaaatt tgtaagtaat
     4261 atttaactgc tcaatacatc taacctttca ggagtcttcg gtttggccaa ctcatcctca
     4321 cgatattccg ttctgactgc cgcccactgg gggcccatgc tggttgaaac cgacggcgaa
     4381 accgtgttta gctcgcgtgg cgcgttagcc acaggaatgg aaaactcctt gcagagcgcg
     4441 gttcgcgacc aggttcacag caatacgcgg gtacgatttc caatggtgcg caaaggcttt
     4501 ctcgcgtcac cggaaaaccc gcaaggcatt cgtgggcagg atgaatttgt tcgcgtgagt
     4561 tgggatgagg cgctggatct tattcaccaa caacataaac gcattcgtga ggcttatggt
     4621 ccggcatcga tttttgctgg ttcctacggc tggcgttcaa acggcgtgct gcataaggcc
     4681 tcgacattat tacaacgcta tatggcgctg gcaggcggtt ataccgggca tctgggggat
     4741 tattcgaccg gtgcggcaca ggcgatcatg ccgtatgtcg taggtggcag cgaagtttat
     4801 caacagcaaa ccagttggcc gctggtgctg gaacatagcg atgtcgtggt gttgtggagt
     4861 gctaacccac tcaatacgct gaaaattgcg tggaatgcat ccgatgagca ggggctttct
     4921 tacttttccg cactgcgtga cagtgggaaa aagctgatct gcattgatcc aatgcgatcg
     4981 gaaaccgtcg atttctttgg cgataaaatg gaatgggtgg caccgcacat gggcaccgat
     5041 gttgcgctga tgctggggat cgcccatacg ctggtggaaa atggttggca cgacgaagcg
     5101 tttctggcgc gttgcaccac aggttatgcc gtcttcgcct cttatttgct gggcgagagt
     5161 gacggaatag cgaaaaacgc cgaatgggcg gcagagattt gtggtgttgg cgcagcgaaa
     5221 atccgcgagc tggcggctat tttccaccaa aataccacca tgctgatggc aggctgggga
     5281 atgcagcgcc aacagtttgg tgagcaaaaa cactggatga tcgtcacgct ggcagcaatg
     5341 ttggggcaaa tcggcacacc cggcggcggt tttggtcttt cttaccattt tgccaatggt
     5401 ggtaacccca cgcggcgttc tgcggtgctc tcttccatgc agggcagctt gccgggtggc
     5461 tgcgatgcgg tggataaaat ccctgttgcc cgcattgttg aagcactgga aaaccctggt
     5521 ggcgcatatc aacacaacgg tatgaaccga catttcccgg atattcgttt tatctggtgg
     5581 gcgggcggtg ccaactttac tcatcatcag gataccaatc gtctgatccg tgcctggcaa
     5641 aaaccggagc tggtggtgat ctctgaatgc ttctggacgg cggcggcgaa acacgcggat
     5701 atcgttctgc ctgcgactac ctcttttgag cgtaatgatc tcaccatgac cggtgattac
     5761 agtaatcagc atctggtgcc gatgaagcaa gtggtgccgc cacgctatga agcgcgtaat
     5821 gattttgatg tttttgccga gttgagtgaa cgttgggaga agggcggcta tgcgcggttt
     5881 acggaaggaa aaagtcagct gcaatggctg gaaacgtttt ataacgttgc ccggcagcgc
     5941 ggggcaagcc agcaggttga attgccgcca tttgctgagt tctggcaagc caaccagtta
     6001 attgagatgc cggaaaaccc ggacagcgag cggtttattc gcttcgctga tttttgccgc
     6061 gatccgctgg cgcatccgtt aaaaaccgcc agcggcaaga ttgaaatctt ctcacagcgt
     6121 attgccgatt acggttaccc ggattgccct aggcatccaa tgtggctgga gccggacgaa
     6181 tggcagggca atgccgaacc agaacagttg caggtacttt ctgcccatcc ggcgcaccgc
     6241 ctgcacagcc agctgaatta cagttctctg cgcgaattgt acgcggtagc aaaccgtgag
     6301 cctgtcacca ttcatcctga cgatgctcag gcgcgcggca taaccgaagg ggatatggtg
     6361 cgggtgtgga actcccgtgg gcagatcctt gccggagcgg tcattagcga gggaattaaa
     6421 cctggcgtga tttgcattca cgaaggggca tggccggatc tggatttaac cgctgacggt
     6481 atttgtaaaa acggcgcagt gaacgtgctg accaaagatc tccccagctc gcggctgggg
     6541 aatggctgtg cgggtaatac ggcgctggca tggctggaaa aatacaacgg tccggaactg
     6601 acacttacag cgtttgaacc accggccagc tcataatcca ggtgggtagt tgggtttcat
     6661 cctgccatgc gcaatcgaca atgtgaaaac cctgcgcacg gtaaaaatct atcgccggtt
     6721 gatttttttg ataaacctcc agcatcagat ggggatagcg ctgctgcaca tactgcatca
     6781 gcgccttacc aataccgcgt ctgacggcct tcggtgcgac aaacattgct gccagaaatc
     6841 ggccttccat aatgctgaca aaaccgagaa gcttaccgtc ctcttcccag acccagtttt
     6901 gcgcgttggc aagataggca tcccgcacca gcggaatgca gtcacgccag taattcgctt
     6961 ttataaaggg atgcccccag gttgtacttt ccagccacag ttcgaggatc gcggggagtt
     7021 ctgaacgttg cgcttcccga atcatggttt atttcccgga tagcaacagc agccaaccac
     7081 atgatcattc accagcccac atgcctgcat aaaggagtaa cagattgtgg tgccgacaaa
     7141 cttaaaacca cgttttttca gtgccttaga tagggcgtcg gaggcggacg tagatgtggg
     7201 aatttcgctc aacgttgtgg cttgtgtcac ctgtggctga tgatttacaa acgaccagac
     7261 aaagtcggga aacggttcgc cgttctgttc catttgcagg tacgcccgcg cattaccaat
     7321 aattgcctga attttccctc gatggcggat aatcccggcg tcctgtacca gtctttcgac
     7381 atcctcttcc tgcattgctg cgaccttcac cggatcgaac tgatgaaagc aggcgcgata
     7441 gttttcgcgt tttttgagga cggtgatcca cgataaccca gcctgctgcc cttcaaggca
     7501 gatcatttcg aacagttttt tactgtcagt ttcaggcacg ccccactcat tatcatggta
     7561 ggcaatataa agcgggtcct gactcaccca gccgcaacgt tccatacttt ccctcgctat
     7621 gtacccgatc gtaaaaattt cagccgcttt tcttgacgca tcggtgaaaa agtcaatatt
     7681 taatacaggg ctactatgcc cgatatcctt catacaatga caataatatt gccgaattcg
     7741 gctcttccct ggagtcgtgt taatgatcat aaaaaaaagc ggtggtcgct ggcagctaag
     7801 cctgctggcg agcgtggtaa tcagtgcctt ttttctcaac acagcttacg cctggcaaca
     7861 agaatatatc gttgatacgc aacccggaca ttccacagag cgttacacct gggatagtga
     7921 tcatcaacct gattacaacg atattttgtc gcaacgtatt caaagtagcc aaagggcgct
     7981 gggactggaa gtcaatctgg cggaagaaac tcctgtggat gtgaccagca gtatgagtat
     8041 gggctggaat tttcctttat atgaacaggt tacaaccggc ccggtcgcgg cattacatta
     8101 cgatggcaca accacctcga tgtataacga gtttggcgac agtactacca cgctgaccga
     8161 tccgttatgg catgccagcg tgagtagctt aggctggcgt gttgactccc ggcttggcga
     8221 tctccgaccc tgggcgcaaa tcagctataa ccagcaattt ggcgagaata tctggaaggc
     8281 gcaatcaggc ctgagccgga tgacggcgac aaaccagaac ggcaactggc tggatgtcac
     8341 cgtaggcgct gatatgttgc tcaatcaaaa tattgccgcc tatgctgcgc taactcaggc
     8401 agaaaatacc actaataata gcgactatct gtatacgatg ggggttagcg ccagatttta
     8461 acgtaacagt cacaattgaa accattgaat aacaacagtt gtggcgatag tgggtgctaa
     8521 cttaccaaat aataaatttg gtgaataatt gtcgcgtcat tcattcctga actaaggcat
     8581 ttcattccgt tctgatggca tttcatgccg tttttcccca ggcataaaat gcacttcgtt
     8641 atggttgtcg gcagagattt ttccttttta ttactgcagg aatactgcca tgacaccttc
     8701 aaattatcag cgtacccgct ggctgacact catcggtact atcattaccc agtttgcgct
     8761 ggggtcggtt tatacctgga gcctgtttaa tggcgcgctt tctgccaagc tggatgcgcc
     8821 ggtaagccag gtcgctttct ctttcggctt gttaagtctg gggctggcaa tttcgtcttc
     8881 tgttgcgggc aaattgcagg aacgttttgg cgttaaacgc gtcaccgtgg cttccggcat
     8941 tttgctggga ttaggcttct tcctgaccgc gcattccaac aacctgatga tgctgtggtt
     9001 aagcgccggt gtgctggtcg gtctggcgga tggcgcgggt tacctgctga cgctctctaa
     9061 ctgcgtgaag tggttcccgg agcgtaaagg tctgatctcc gcgttcgcta tcggttctta
     9121 tggtctgggt agcctgggtt tcaaatttat cgacacgcag ctgctcgaaa cggtgggtct
     9181 ggaaaaaacc tttgtgattt ggggagcgat tgcgctggtg atgattgttt tcggcgcaac
     9241 gttaatgaaa gacgcaccaa aacaggaagt gaaaaccagc aatggtgtgg tggagaaaga
     9301 ttacacgctg gcagagtcga tgcgtaaacc gcagtactgg atgttagcgg taatgttcct
     9361 gactgcctgc atgagcggcc tgtacgtgat tggggtagcg aaagatatcg cccaaagtct
     9421 ggcacacctt gatgtggttt ccgcagccaa tgcagtaacg gttatttcca tcgctaacct
     9481 ttcaggtcgt ctggtgctgg gcattctgtc tgacaaaatt gcccgtatcc gtgttatcac
     9541 cattggtcag gtgatttcgc tggtgggtat ggcggccctg ctgtttgcac cattaaatgc
     9601 agtgacgttc tttgcagcga ttgcctgcgt ggcatttaac tttggcggca ctattaccgt
     9661 ctttccatca ctggtcagtg agttcttcgg cctcaataac ctggcgaaaa actacggtgt
     9721 gatttacctc ggtttcggta tcggtagcat ttttggttcg attatcgcct cactgtttgg
     9781 cggtttctat gtgaccttct acgtgatttt cgccctgctg attctgtcat tggcgctttc
     9841 tacgacgatt cgtcagccag agcagaaaat gttgcgtgag gcgcatggct ccctttaatg
     9901 ctcagccgcg agctactggg cttcagcttc cgtgtgcatc aaacgcatca acgaaatgta
     9961 taaacaatga ctcgtaaaat ttgggccagt taactggccc tttttactgc tttttgtaac
    10021 gctaaagtct cttttcaaac ttgcattttt gtaaatttgt gcttcatgca cactctttcc
    10081 ccacactttt tcgctttgct gtggtctact tattcgcgcg tgtagatttt acttatctga
    10141 ctacctccgc acttttcccc tgccgggcct gaaaagccgc taagcagggt gttatcacct
    10201 gtttgtccag ggtttgtttg catgagatac atcaaatcga ttacacagca gaagctgagc
    10261 tttttgcttg caatctatat tggccttttt atgaatggcg cggtttttta tcgccgcttc
    10321 ggcagctatg cgcacgattt taccgtctgg aaaggcgttt ctgctgttgt tgaactggcc
    10381 gccaccgtac tggtgacctt ctttttacta cgtcttctat cgctgtttgg ccgccgcagc
    10441 tggcgtattc tggcgtcgct ggtggtgctc ttttccgcag gtgccagcta ttacatgacc
    10501 ttccttaatg tggtcattgg ttatggcatc atcgcttccg tcatgaccac cgatatcgac
    10561 ctgtcaaaag aagttgttgg tctgaacttt attctctggt taatcgccgt tagtgcattg
    10621 cctcttatcc ttatctggaa taaccgctgt cgctacacct tgctccgaca actgcgaacc
    10681 ccagggcagc gtattcgcag cctggcggtc gtcgtactgg cgggtattat ggtttgggca
    10741 ccgattcgtt tgctggatat ccagcagaag aaagtggaga gggcgaccgg cgttgatttg
    10801 ccgagttatg gcggtgtcgt agcgaactct tatctgccat caaactggct ttctgcgttg
    10861 gggctgtatg cctgggcgcg ggtcgatgaa tcttccgata ataattcatt gcttaatccg
    10921 gcgaagaagt ttacatacca ggctccacaa aacgttgatg atacttatgt cgtgtttatc
    10981 atcggtgaaa ccacgcgttg ggaccatatg ggtattttcg gctatgagcg taataccacg
    11041 ccgaaactgg cccaggagaa aaatctggcg gcgttccgtg gttactcctg tgataccgca
    11101 accaaactct cactgcgttg catgtttgta cgtcaggggg gcgcggaaga taatccgcag
    11161 cgcacattaa aagaacagaa cattttcgcg gttctgaagc agttaggatt cagttctgac
    11221 ctctacgcta tgcagagcga aatgtggttc tacagcaaca cgatggcgga caacattgct
    11281 tatcgtgagc agattggtgc ggagccacgt aatcgtggca agccggtaga tgatatgttg
    11341 ctggtagatg aaatgcagca atcgctaggg cgcaacccgg atggtaagca tctgatcatt
    11401 ctgcatacca aaggttcgca ttttaactac acccagcgtt atccgcgtag cttcgcgcag
    11461 tggaagccgg aatgtattgg tgttgatagc ggctgtacca aagcgcagat gatcaactcc
    11521 tatgacaact cggtgaccta tgtggatcac tttatctcca gcgtgattga tcaggttcgc
    11581 gataagaaag cgattgtgtt ctacgcagct gaccacggtg agtcaattaa tgaacgcgag
    11641 cacctgcacg gcacgccgcg tgaactggca ccgccggagc agttccgcgt accgatgatg
    11701 gtctggatgt cagataaata tctggaaaat ccggccaatg cgcaggcgtt tgcgcagctg
    11761 aaaaaagaag ccgacatgaa agtgccacgc cgtcacgtag agctgtacga caccatcatg
    11821 ggttgtcttg gctatacttc accggatggt ggaattaacg aaaacaacaa ctggtgtcac
    11881 atcccgcaga caaaagtggc agcggctaac taagcggcct gctgactttc tcgccgatca
    11941 aaaggcattt tgctattaag ggattgacga gggcgtatct gcgcagtaag atgcgccccg
    12001 cattcggtga ttggcgcagc ctggtagcgc acttcgttcg ggacgaaggg gtcggaggtt
    12061 cgaatcctct atcaccgacc aaattcgaaa agcctgctca acgagcaggt ttttttgcat
    12121 ttgtagctca tgaggatgag aacctccggg ggcagggagg ctcgactcga cgtaggcctg
    12181 ataagacgcg ccagcgtcgc atcaggcaac ggcaccgcac ttattgtcgg atgcggcgcg
    12241 accgccttat tcgaccaaca caccacacat tttcctgcca actttgcgcc ccatatctca
    12301 ctatccatta acggatttgt gacagaaacc attgtctttt tttatatttg cttaaatctt
    12361 ttttgcgtta cttatggctg gcgctggcat tttccgctgt gcgttttagc gttcatggta
    12421 ttaatcgctc agatatccac cttttcctca gatttttttc attaaatgca taaaacttaa
    12481 tcaccaaatg ttgctaaata ttaagctgat tgttctggtt atgttggagt agcacaaatt
    12541 tctctgctgc aaatagctgt ttgaagtttt tttaatcttt gtttgtggat tctcaccgtt
    12601 ggtgtaaatc ccggttggct gtattgacgt tttcacattc tgttgacaga ttgtaggtca
    12661 cgaggggcat tttatggagg atccgcactg ttacactgat gttaattagt acggcatccc
    12721 cacctcataa cgttgacccg accgggcaaa aaacaaaaaa ggtcaggcag cgacaaccca
    12781 ttgcaaaggg ttaaaacaac aaacatcaca attggagcag aataatgcgt atttccttga
    12841 aaaagtcagg gatgctgaag cttggtctca gcctggtggc tatgaccgtc gcagcaagtg
    12901 ttcaggctaa aactctggtt tattgctcag aaggatctcc ggaagggttt aacccgcagc
    12961 tgtttacctc cggcaccacc tatgacgcct cttccgtccc gctttataac cgcctggttg
    13021 aatttaaaat cggcaccacc gaagtgagcc cgggcctcgc tgaaaagtgg gaagtcagcg
    13081 aagacggtaa aacctatacc ttccatctgc gtaaaggtgt gaagtggcac gacaataaag
    13141 aattcaaacc gacgcgcgaa atgaacgccg atgacgtggt gttctcgttc gatcgtcaga
    13201 aaaacgcgca aaacccgtac cataaagttt ctggcggcag ctacgaatac ttcgaaggta
    13261 tgggcttgcc ggagctgatc agcgaagtga aaaaggtgga cgataacacc gttcagtttg
    13321 tgctgactcg accggaagcg ccgttcctcg ctgacctggc aatggacttc gcctctattc
    13381 tgtcaaaaga atatgctgac gcgatgatga aagccggtac accggaaaaa ctggacctca
    13441 acccaatcgg aaccggtccg ttccagttac agcagtacca aaaagattcc cgtatccgct
    13501 acaaagcgtt tgatggctac tggggcacca aaccgcagat cgatacgctg gtcttctcta
    13561 ttacccctga tgcttccgtg cgttacgcga aattgcagaa gaatgaatgc caggtgatgc
    13621 cgtacccgaa cccggcagac atcgctcgca tgaagcagga taaatccatc aacctgatgg
    13681 aaatgccggg gctgaacgtc ggctacctct cgtataacgt gcagaaaaaa ccgctggatg
    13741 acgtgaaagt tcgccaggca ctgacctacg cggtgaacaa agacgcgatc atcaaagcgg
    13801 tttatcaggg cgcgggtgta tcagcgaaaa acctgatccc gccaaccatg tggggctata
    13861 acgacgacgt tcaggattac acctacgatc ctgaaaaagc gaaagccttg ctgaaagaag
    13921 cgggtctgga aaaaggtttc tccatcgacc tgtgggcgat gccggtacaa cgtccgtata
    13981 acccgaacgc tcgccgcatg gcggagatga ttcaggcaga ctgggcgaaa gtgggcgtgc
    14041 aggccaaaat cgtcacctac gaatggggtg agtacctcaa gcgtgcgaaa gatggcgaac
    14101 atcagacggt aatgatgggc tggactggcg ataacgggga cccggataac ttcttcgcca
    14161 ccctgttcag ctgcgccgcc tctgaacaag gttctaacta ctcaaaatgg tgctacaaac
    14221 cgtttgaaga tctgattcaa ccggcgcgtg ctaccgacga ccataacaaa cgcgttgaac
    14281 tgtacaagca agcgcaggtg gtgatgcacg atcaggctcc ggcactgatc atcgctcact
    14341 ccaccgtgtt tgaaccggta cgcaaagaag tcaaaggcta tgtggttgat ccattaggca
    14401 aacatcactt cgaaaacgtc tctatcgaat aattaaaagc catacaagac tgatggcaaa
    14461 ggcaaaactg cctgatgtgc tccgcttatc aggcctacga aaattctgca atgtattgaa
    14521 tttgcacgat tttgtaggcc ggataaggcg ttaacgccgc atccggcata aacaaagcgc
    14581 actttgtcaa caatctgtat acccggtggc gctgtgcctc tgcgtgcagc gccatccggc
    14641 agcaatactt cattctctgt ccgacaaggg cgggaattga tttgtgagca atacagacac
    14701 gcagttccag gctgcgggtc actatagaga atccgggtta tgttgcagtt tattctccga
    14761 cgtttgggac tcgtcatccc cacgtttatc ggtattaccc ttctcacatt tgcctttgtc
    14821 cacatgatcc cgggcgatcc ggtgatgatc atggcaggcg aacgtgggat ctcccctgag
    14881 cgtcacgtgc agctgctggc tgaactcggc ttagataaac cgatgtggca gcagtatctc
    14941 cattacattt ggggcgtaat gcacggcgat ctaggcattt caatgaaaag ccgaattcca
    15001 gtatgggaag agttcgtgcc gcgctttcag gccacgctgg aacttggcgt ctgcgcgatg
    15061 atttttgcta cggcagtcgg tattccggtt ggtgtgctgg ctgcggttaa acgcggttcc
    15121 attttcgatc acactgcagt tggcctggcg ctgaccggct actcgatgcc gatcttctgg
    15181 tggggcatga tgctgatcat gctggtttcg gtgcactgga acctgacgcc cgtctccggt
    15241 cgcgtgagcg atatggtgtt cctcgatgac tccaatccgt taaccggttt tatgctaatc
    15301 gacaccgcca tctggggtga agacggcaac tttatcgatg ccgtcgccca tatgatcttg
    15361 cccgccattg tgctgggtac tattccgctg gcggtcattg tgcgtatgac acgctcctcg
    15421 atgctggaag tgctgggcga ggattacatc cgcaccgcgc gcgccaaagg gctaacccgc
    15481 atgcgggtga ttatcgtcca tgcgctgcgt aacgcgatgc tgccggtagt gaccgttatc
    15541 ggcctccagg tgggaacatt gctggcgggg gcgattctga ccgaaaccat cttctcgtgg
    15601 cccggtctgg ggcgctggtt gattgacgca ctgcaacgcc gcgactatcc ggtagtgcag
    15661 ggcggcgtat tgctggtggc gacgatgatt atcctcgtca acttgctggt cgatctgctg
    15721 tacggcgtgg tgaacccgcg tattcgtcat aagaagtaag gggacatcat gtcacaggtt
    15781 actgaaaata aagtgattag cgcaccggtg ccgatggccc cgttacagga gttctggcac
    15841 tattttaaac gcaacaaagg cgcggtcgtc gggctggttt acgtcgccat cgtgctgttc
    15901 atcgcgatct ttgccaactg gattgcgcct tataacccgg cggatcagtt ccgcgatgcg
    15961 ctgctcgccc cgccagcctg gcaggaaggc ggcagtatgg cacacttgct gggcaccgat
    16021 gacgtcggtc gtgatgtgct gtcgcggctg atgtacggcg cgcgtttatc gctgctggtt
    16081 ggctgtctgg tggtggtgtt gtcgctgatt atgggcgtta ttctcggcct gatcgccggt
    16141 tacttcggcg gcctggttga taacattatc atgcgcgtgg tcgatatcat gctggcgctg
    16201 ccaagtctgc tgttggcact ggtgctggtg gcgattttcg gcccgtcgat tggtaacgcc
    16261 gcgctggcgc tgaccttcgt tgccttgccg cactatgtgc gcttaacccg cgccgccgtg
    16321 ctggtggagg tgaatcgcga ttacgtcacc gcctctcgcg tggcaggtgc cggggcgatg
    16381 cgccagatgt ttattaacat cttcccgaac tgccttgcgc cgctgattgt tcaggcgtcg
    16441 ctcggtttct ctaacgccat tctcgatatg gctgctcttg gtttcctcgg catgggggca
    16501 cagccgccaa cgcctgagtg gggcaccatg ctctccgacg tgttgcagtt cgcgcaaagc
    16561 gcctggtggg tcgtgacctt ccctggtctg gcgatcctgc tgacggtgct ggcatttaac
    16621 ctgatgggtg acggtctgcg tgacgcgctc gatcccaaac tgaagcagta agaggttcga
    16681 gatggcgtta ttaaatgtag ataaattatc ggtgcatttc ggcgacgaaa gcgcaccgtt
    16741 ccgcgccgta gaccgcatca gctacagcgt aaaacagggc gaagtggtcg ggattgtggg
    16801 tgagtccggc tccggtaagt cggtcagttc actggcgatt atggggctga ttgattatcc
    16861 gggccgcgta atggcagaaa aactggagtt taacggccag gatttgcagc gtatctcaga
    16921 aaaagagcgc cgcaacctgg tgggtgccga agtggcgatg atcttccagg acccgatgac
    16981 cagccttaac ccgtgctaca ccgtgggttt ccagattatg gaagcgatta aggtgcatca
    17041 gggcggcaac aaaagtaccc gccgtcagcg ggcgattgac ctgctgaatc aggtcggtat
    17101 tcccgatccg gcttcgcgtc tggatgttta cccgcatcag ctttccggcg gcatgagcca
    17161 gcgcgtgatg atcgccatgg cgattgcctg tcggccaaaa ctgctgattg ccgatgaacc
    17221 gaccaccgcg ctggacgtga ccattcaggc gcaaatcatc gaactactgc tggagctaca
    17281 gcagaaagag aacatggcgc tggtgttaat tacccatgac ctggcgctgg tggcggaagc
    17341 tgcgcataaa atcattgtga tgtatgcagg tcaggtagta gaaacgggcg atgcacacgc
    17401 catcttccat gcgccgcgtc acccgtatac tcaggcattg ctgcgtgcgc tgccagaatt
    17461 tgctcaggac aaagaacgtc tggcgtcgtt gccaggtgtt gttcccggca agtatgaccg
    17521 cccgaacggc tgcctgctta acccgcgctg cccctatgcc actgacagat gtcgcgctga
    17581 agaaccggcg ctgaatatgc tcgctgatgg gcgtcagtcc aaatgctatt acccacttga
    17641 tgatgccggg aggcccacac tatgagtacg caagaggcca cctcgcaaca accgctgttg
    17701 caggctatcg acctgaaaaa acattatccg gtgaagaaag gcatgttcgt gccggaacgt
    17761 ctggtgaagg cgctggacgg cgtttcgttt aaccttgaac gtggcaaaac gctggcagta
    17821 gtgggcgaat ctggctgcgg taaatcgacc ctcggtcggt tgctgacgat gatcgaaacg
    17881 cccaccggcg gcgagctgta ttatcagggg caggatctgc tcaagcacga tccgcaggcg
    17941 cagaagctgc gtcggcagaa aatccagatc gtcttccaga atccctacgg ttcgctgaat
    18001 ccgcgtaaga aagttgggca aattctcgaa gagccgctgc ttattaacac cagcttaagc
    18061 aaagaacagc gtcgggaaaa agccctgtcg atgatggcaa aagtcggcct gaaaaccgag
    18121 cactatgacc gctatccgca catgttttcc ggcggacaac gtcagcgtat cgctattgcc
    18181 cgtggtctga tgctcgaccc ggatgtggtg attgccgatg agccggtttc tgcgctggac
    18241 gtctcagtgc gtgcgcaggt gctgaatctg atgatggatt tgcagcagga gttggggctt
    18301 tcttatgtct ttatctccca cgacctgtcg gttgtggagc acattgctga tgaagtgatg
    18361 gtgatgtacc taggccgctg cgtggagaaa ggaacgaagg accaaatctt caataacccg
    18421 cgccatccgt acacccaggc gctgctctcc gcgacgccac gcctgaaccc ggacgatcgt
    18481 cgtgaacgca tcaaactcac cggtgaactg ccaagcccgc tgaatcctcc gccgggttgc
    18541 gccttcaacg cccgctgtcg tcggcgcttc ggcccctgca cccagttgca gccgcagcta
    18601 aaagactacg gcggtcaact ggtagcttgt tttgctgttg atcaggatga aaatccgcag
    18661 cgttaactca caaaaccggg gccatgcgct ccggtttact caatcagctt caacagtggc
    18721 gacaagcata gcaaaatgcc gtaaagcaga atcagccagg ctttaaatcc gcgaagtttt
    18781 tccagttgtg cgactttata gatcaggaaa aacggaatga ggcacgacac aatgccatat
    18841 aacgggcttc ccaactgaaa gaacaccagc actgatacac gaaacgaaac ccaaatcgtc
    18901 aacgtaatga cgataaaagc gcagatcgcc agagtcagca tgcgtgagtt aattttcttg
    18961 gtatcaataa ttcggcttaa cagattgaga ataatgcctt taatagcctc gtggaaaccg
    19021 agataaatgc caaagaatgc ggtcagtacg gcaaagatat tcagcaccgt agaggtgata
    19081 tgaatgatat gtccagggat cacctgcgcg gccagtgcca gtgccgagat attttgttca
    19141 aaggcagaaa cggcttcttc gtggctaatt gagaaggtaa acgaaaaggc aaaaaacagg
    19201 atcaccgcga tgagcgtgat ataactaatt cggtgggtac gcagcgcgag ccgggttgcc
    19261 agtactttat ctgcttcccg tttacgatag gcaatattca ttgggttaag tacctgaata
    19321 aataccgcag aaaagaagca aaatggaatg gtaagcagga catcgcggaa aaagacggag
    19381 gcttgcggga aggcggttat attggcgaaa ttccagtgcg ggatcatcgc aaaaccgaac
    19441 acgacaataa tccctacttt gaccaccacc attggcccag aaatcttaaa cagtaatcgt
    19501 tcaccaccag acgcaatcgc tatcagtacg gcgaaaatag cgactttata aagtagagat
    19561 tgtgaaagat cggcatcggt taaaccgaag gttttcaggt acgaggcgct gtcgaaaacc
    19621 acggagagag agtagataaa aatcccgtgg ataatcatca aaaagtagat aaccccgagg
    19681 aaaattcccc agttcttccc caggtaatga ctgataatat cggtgtagtc attacaggaa
    19741 tcgctttcag aaagggtttt taaataaatg tcctgcacta cccaggtggc aggataagca
    19801 atgatcgctg cggtaataaa tacccaaatt cccttcaagc caatttgtac tggcatcagc
    19861 acggttccgg caccaatcgc catgcctatg cataataaaa cccagccgaa gtcgtagcgt
    19921 gtaaacggca atttctgatt gtgtttcgat agtgtgttgt gctgcataaa gtcctgtcca
    19981 gaaagaagtc ggcctcgcaa aaatgaataa acccagtttc tcttgcgcaa caggtggaga
    20041 agtcgaatac gcagtatgta aaaaatgagg caacagattc actggggagg tcgcgaggag
    20101 gcgcaattct acaaaattgc gcatttcact accagtctgg agtcagcccg gatatgaact
    20161 gcaactattg caaataaaga aattaagctg gaaggcatcg cgcaatgagg aagattgccg
    20221 ggtgctgaca gtaaaaagca atcgtagaat cagcggcaag ccggaaaggt tccggtgagg
    20281 cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggagagag aaaacccccg
    20341 cacgttgcag gtatgcacct gacaacatca cgggggctaa tcttgaaccc aaacaactca
    20401 agaatagccg cgaaacgttg gcattacaac acaggcggct atatgacgct cgcagaactg
    20461 ggcatggcct tctggcatga tttagcggct ccggtcattg ctggcattct tgccagtatg
    20521 atcgtgaact ggctgaataa gcggaagtaa cgtgtcatgc gggcgtcagg ctgccgtaat
    20581 ggcaatttgc gcccggatca ggccgcagag gttaaactct gcggcctttt tcgtgcttac
    20641 tgcgggtaag gcacccagtc gccgccgttc aggcgaacgt acggtttatc ttggtattga
    20701 ataactactg catttgagtt ctcggagacc ggtgctgttt gtggcaaccc actggtgagt
    20761 tttttccagt caacattgtc ttcggtgaaa atcttgccat caagaacgcg aaccaccaga
    20821 tcggagatag ccaggaagct gctcggttgg tcaatgacaa tcggtgcccc ctgatgtggt
    20881 gccttcatgc cgaagaattt caccccaacg gggacgtctg tgatagacgg gctagggata
    20941 tcacgtaggc cagatacctg cattctgtcg cccttcagcg cgccgccgtg ttccggcacc
    21001 acgaccacca tcactttacg acccgatttc tccagttcag taaagaaggc gtccagttca
    21061 tcaaagaatt tctgcgcccg cgctttgtaa tccgctgttt tgctgacccc cggataatgg
    21121 ttgccgtcat gcagtggaag cgtgttgtag aacgtggcac tacggctgtt tttatctttt
    21181 tcggtaacgt ccagccagcg gttaagcacg gcggtatcgt cataaaccgg cgaaccatca
    21241 aagcccagca aaataaccgg cagatttgtt tgatccatca attcagtctg catgccgcca
    21301 ttttcgcgaa cttctttcaa aaaaccgccg aactggccgt tatgtcccat catcaggtgc
    21361 tgggtaaagc ccagtttcga aaggttatca aacagatagc agtcgttatt tgccggttga
    21421 tacagattag tgtgcgaagt ctgcccgcag ctggcgcgca gtaaacggat cgccgccggg
    21481 ccactgtagg aggtggcgga gttaaagttc ttgaactcaa tatcgaaatg cgaccacagt
    21541 ggatgcgaca tcaacccggc ggcttctata tccgaccagg aaagcgaaca gatgttaatc
    21601 accagtagtt caaatggctg agcatcagcg ggcagcgaag acgggaaggt cgatttacgt
    21661 ttcgcctccg cgttatagaa attattcagc caggcgttaa ggttcgccgt tgttggcggt
    21721 gcagtttgtg cgggcatatc acccactacc ggtgcgccac ccgtcgccgc aacggttgcc
    21781 gctgcgttac cacccgtcgt tgttacagtg gtcgtcggtt gtccggctgg ccacaaggag
    21841 aaacttggtc ccgccagggt aagtacgttc agccatagca gtatggcaac cacaaaaacg
    21901 gtaatgcgaa tccattgtga caggaataac caggccacta ataaaacaaa aatggcccca
    21961 atcatctgcc agttaataaa gcgtgtgaca aggtcgatta aataatcggt actgaacccc
    22021 gccacctgcg aaccctggct cattatgctt tccgggccag gcaaccaggt gtcatgccag
    22081 aacaaagcaa agccgatcgg cagggcaatc cagtggcgca agcgatgcag gctgtaacgc
    22141 ggaatgggca tcagcagaaa cgcggcaaac accaaattga ggagcgggtg gaagttaaga
    22201 tatcccgccc acaacaggcc aaacttaacc agaaaataga agttccagcc ggaaaggccg
    22261 cgccagtatt gccagaggga agaaggcatg gcggtatttt gcgtaaattg agtcattttt
    22321 tggttgccct ggctttttcc gtgcggcgta acgtcccggc cggtttaaca taacgaggtt
    22381 tagcaaagaa caaacgtaag gtgtcgcgaa tgcgtcgcaa agagtgccgc gccagatagc
    22441 ccagcgggaa aaatatcagt gcgcaaacga caataatttc aatgatatcg ctgatggtca
    22501 tcatgatgag cgctccacag catcatctaa cagtcgcatg ggttctggta ttcgtcgcca
    22561 gtggcgacca tcgtgctcgg cattgatgac cggtttagaa ctttgcgtta aaggcagcgg
    22621 cattccccat tgttctgggg caagcaggcg catctgcacc agctcggcac tgatttgatc
    22681 atcttcaaac cagaccatac ggtttgagaa aatgtcgcca gtaggcaatg ggaaaatatg
    22741 attcaacgcg gtatccagat cgttaatccg acagaatgag agaaacagca ccagccgatt
    22801 accgccaatg gtcatgatat cgccggtacg gttagggcga catagcgtct gggcttgctc
    22861 aacgcggata cccggtaccg gacgtagggc aaccagaacg cctttaccgt gggcaggcaa
    22921 gagagggtta ttcatcatgt tgttgacggc attacagaac acatcccact tctggaaacc
    22981 acgcagtttg agcggctggg tcattgacag caaggtagtg atatcttccg gtacatagcg
    23041 actaaacttc tgcccttgca cgctttcgat catcgtcaga caacgggaga gtggcgcatt
    23101 ccacggaata accatatttg caccgcaggc caataacaaa cgttcatcgg tggcgcgcag
    23161 gctagcggta ttttcccgca cgaggatttt catcgcacta ccgcgctggc gacgcagggt
    23221 atgaatgctg cgggccagtg gctcgatttg cgcattttgc tgtaaagaaa agaccaccgt
    23281 cgccgcctga gcggtacggg cttcattgaa caggacttcg ttattgttga acagttgcca
    23341 gtgttccgat agcggcggcg caccttccag tacagcaaca ttactcagaa tgcgtttttc
    23401 gtcgctgcgt ggttggatct ccgcctcttc gctttgaact aatgtccaga taccattttg
    23461 ttgctgaacg ctaagctgct gacgggcgct gacccctttt tcgttgcacc agaaggcaat
    23521 atccagcaaa tgttggtcac cctgaaaacg caaactggca agaccaaaaa gtgaacggta
    23581 ttcctcaagc aacaatgaaa attgtttatc gttattattt ccgggattaa ttaccaaaag
    23641 cgaacaatgg tttaacctgc tccatttatt cattttatcc aaccatgagc gaagccgctc
    23701 ggcaggaatg ttttgccatg cgttatttgc gcaaacaaga ataaaaaggt aattatgggg
    23761 atcaatagaa cactgcaaat cacgggccaa atagtataga cctttttcat gatttagcat
    23821 tgaaaataat tttatttttt ccggcccttg agaatcatct aattgaaaga ttttcgccgg
    23881 atcgctgtcc atgctaatga ccgcgacgtg tgcggtttca gcctgggatg caattgtttg
    23941 attcgccaga ctgatagcat cttcatggcg atcgacgtta aaccaccaga cgccgcctgc
    24001 tggcatatgt cgcagctcat cccataatga tgagataccg atagagaata cagggtccac
    24061 aatgtccctc atgtcaccat tttgtctgat tatcagttta ctagcgaaag ctaagaaata
    24121 aacctaccat tgaaattaaa acttatcaga tttagcatgt gaatcaattt agaaggtcac
    24181 aagacgtgtg attttagtct tttcgttttt aactttttct gttgcgtaac ggaaagtcaa
    24241 aaagtgagca aattcccgtc tcgccgtaaa caataaaccg gcgaaatgct cacggttaga
    24301 attgtctcat gaacggtacg gttatttcat agggatcaag caaaatgaat aacaatgaac
    24361 cagatactct gcctgatccc gcgataggct atatcattcc agaatgatat tgtggcgtta
    24421 aagcaggcat tttcactgcc tgatattgat tatgccgata tttcccaacg cgaacagttg
    24481 gccgcggcat taaaacgctg gccgttgctg gcagagtttg cgcaacaaaa gtaggggatt
    24541 ggtgaatggc cgtactggga ttgcaggggg tgcggggagg cgtggggaca acaaccatca
    24601 ccgccgcatt agcctggtca ttacaaatgt tgggagaaaa tgtcctggtg gtcgatgcct
    24661 gcccggacaa cttgttgcgc ctgtcattta atgttgattt tacccaccgt cagggctggg
    24721 ccagagcgat gctggatggc caggactggc gtgacgctgg gttgcgctac acctcgcagc
    24781 tcgatttgct gccttttggt cagttatcca ttgaagaaca agaaaatcca cagcactggc
    24841 aaacccggct gagcgatatt tgctccggct tacagcaact aaaagccagc gggcgttacc
    24901 agtggatttt aatcgactta ccgcgtgatg cctcgcagat aacccaccag ctgctgagtt
    24961 tgtgcgagca ctcgctggca atcgtcaatg tggatgccaa ctgccatatc cgactgcatc
    25021 agcaagcgct gccggatggc gcacatattt tgattaatga cttccgtatt ggcagtcagg
    25081 ttcaggacga tatttaccag ctttggttgc aaagccagcg ccgattactg ccgatgctca
    25141 ttcatcgtga tgaagcgatg gctgaatgcc tggcggctaa gcaaccagta ggtgaatatc
    25201 gcagtgatgc gctggcggct gaagagatac tgacgctggc gaactggtgc ctgttgaact
    25261 actccgggct gaaaacgcca gtcgggagtg catcatgagt atcctgaccc ggtggttgct
    25321 tatcccgccg gtcaacgcgc ggcttatcgg gcgttatcgc gattatcgtc gtcacggtgc
    25381 gtcggctttc agcgcgacgc tcggctgttt ctggatgatc ctggcctgga tttttattcc
    25441 gctggagcac ccgcgctggc agcgtattcg cgcagaacat aaaaacctgt atacgcatat
    25501 caacgcctcg cgtccgcgtc cgctggaccc ggtccgttat ctcattcaaa catgctggtt
    25561 attgatcggt gcatcgcgca aagaaacgcc gaaaccgcgc agcgggcatt ttcaggtctg
    25621 caaaatattc gtggacgtta ccatcaatgg atgaacgagc tgcctgagcg cgttagccat
    25681 aaaacacagc atctggatga gaaaaaagag ctcggtcatt tgagtgccgg ggcgcggcgg
    25741 ttgatcctcg gtatcatcgt caccttctcg ctgattctgg cgttaatctg cgttactcag
    25801 ccgtttaacc cgctggcgca gtttatcttc ctgatgctgc tgtggggggt agcgctgatc
    25861 gtacggcgga tgccggggcg cttctcggcg ctaatgttga ttgtgctgtc gctgaccgtt
    25921 tcttgccgtt atatctggtg gcgttacacc tctacgctga actgggacga tccggtcagc
    25981 ctggtgtgcg ggcttattct gctcttcgct gaaacgtaca cgtggattgt gctggtgctc
    26041 ggctacttcc aggtagtatg gccgctgaat cgtcagccgg tgccattgcc gaaggatatg
    26101 tcgctgtggc cgtcagtgga tatctttgtc ccgacttaca acgaagatct caatgtggtg
    26161 aaaaatacca tttacgcctc gctgggtatc gactggccga aagacaaact gaacatctgg
    26221 atcctcgatg acggcggcag ggaagagttt cgccagtttg cgcaaaacgt gggggtgaaa
    26281 tatatcgccc gcaccactca tgaacatgcg aaagcgggca acatcaacaa tgcgctgaaa
    26341 tatgccaaag gcgagttcgt gtcgattttc gactgcgacc acgtaccaac gcgatcgttc
    26401 ctgcaaatga ccatgggctg gttcctgaaa gagaaacagc tggcgatgat gcagactcca
    26461 caccacttct tctcgccgga cccgtttgaa cgcaacctgg gacgttttcg taaaacgccg
    26521 aacgaaggca cgctgttcta tggtctggtg caggatggca acgatatgtg ggacgccact
    26581 ttcttctgcg gttcctgtgc ggtgattcgt cgtaagccgc tggatgaaat tggcggcatt
    26641 gctgtcgaaa ccgtgactga agatgcgcat acttctctgc gtttgcaccg tcgtggctat
    26701 acctccgcgt atatgcgtat tccgcaggcg gcggggctgg cgaccgaaag tctgtcggcg
    26761 catatcggtc agcgtattcg ctgggcgcgc gggatggtac aaatcttccg tctcgataac
    26821 ccgctcaccg gtaaaggact gaagtttgct cagcggctgt gctacgtcaa cgccatgttc
    26881 cacttcttgt cgggcattcc acggctgatc ttcctgactg cgccgctggc gttcctgctg
    26941 cttcatgcct acatcatcta tgcgccagcg ttgatgatcg ccctattcgt gctgccgcat
    27001 atgatccatg ccagcctgac caactcgaag atccatcgcc actctttctg gagtgaaatc
    27061 tacgaaacgg tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg
    27121 cacaaaggca aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac
    27181 tgggtgatct cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta
    27241 ggcatctggc gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg
    27301 gtgtgggtgt tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc
    27361 aaacaggtac gccgatcgca ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa
    27421 gatggtcacc tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag
    27481 atcaacggtc aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt
    27541 cagcaggaat acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg
    27601 aaattaatgc cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt
    27661 gcggatacat gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg
    27721 ctggatattc tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct
    27781 tcggtgaagg gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt
    27841 ccgcgccgcc cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa
    27901 tgatgataac gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag
    27961 tgcgttcccc tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga
    28021 gccagctgta gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga
    28081 gcagggagct gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac
    28141 ctttgcgcaa atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg
    28201 cagcattgag tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacctcga
    28261 atacacgcca tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga
    28321 tgagctgatg ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca
    28381 aatgcccatt aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg
    28441 ccattatcag gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg
    28501 gagcagtgga ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt
    28561 cccggtgcca ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc
    28621 gggtgcgccg gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc
    28681 gcgttctggc tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa
    28741 tgccattgtc tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt
    28801 aaaagccccg gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt
    28861 ggtgtttggt cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa
    28921 cattctgttc cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa
    28981 gccgtacgat gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa
    29041 aacctatgaa gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct
    29101 aaacctgccg ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta
    29161 ccgctacacc atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca
    29221 gttcctgcaa tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat
    29281 tccggtatta caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact
    29341 gggcgcgacc aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc
    29401 ggtagataac tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc
    29461 caccatcgac ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc
    29521 taacgcgggc ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc
    29581 gaaagcgcct aacgaagcac agatggaaac gttgctgagt actgttggtt ttatcggcgc
    29641 acagacgggc ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg
    29701 caaagatgcc gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca
    29761 gatcgaccta ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc
    29821 gttccccggc attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac
    29881 ctcttccggt gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag
    29941 cgtgattgcg ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa
    30001 cgatagcggc aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat
    30061 caacagccta cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt
    30121 gtggtatgcg ctggcaaacc atccgattct gctggcggtg ctggcggcta tcagtgtgat
    30181 attgctggca tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc
    30241 ggataacgag taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt
    30301 ggctgccttt agtgttcagg cagcctgtac cgggcctgcc tgggagcagt ttaaaaagga
    30361 ttacatcagt caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc
    30421 cgaagggcaa agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga
    30481 taatattctc gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc
    30541 cgcctggctg tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc
    30601 ctccgatggt gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga
    30661 gcagcgttat accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt
    30721 gacggtgcct gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa
    30781 cagctggcgt tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt
    30841 tggcgcgccg tggaccacgc tgcgcgaaac caatcaacgt ttattgctgg aaaccgcccc
    30901 gaaaggcttt tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc
    30961 cgaaaaaaca ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat
    31021 gcctgacagc gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt
    31081 cactgagaaa aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg
    31141 taaaggaccg gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca
    31201 ggcagttcag cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta
    31261 tgtgctgacc ctgtttggac aaggctggga tcaacatcgt ttccgcttct cgacaaaagg
    31321 tgagttatta cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt
    31381 tccctcggtc tggccgtcat gccgatggtc gaggcagcac caaccgctca gcaacagttg
    31441 ctggagcaag ttcggttagg cgaagcgacc catcgtgaag atctggtgca acagtcgtta
    31501 tatcggctgg aacttattga tccgaataac ccggacgtcg ttgccgcccg tttccgttct
    31561 ttgttacgtc agggcgatat tgatggcgcg caaaaacagc tcgatcggct gtcgcagtta
    31621 gcgccgagtt caaatgcgta taaatcgtcg cggactacga tgctactttc cacgccggat
    31681 ggtcgtcagg cactgcaaca ggcaagattg caggcgacta ccggtcatgc ggaagaagct
    31741 gtggcgagtt acaacaaact gttcaacggt gcgccgccgg aaggtgacat tgctgtcgag
    31801 tactggagta cggtggcgaa aattccggct cgccgtggcg aagcgattaa tcagttaaaa
    31861 cgcatcaatg cggatgcacc gggcaatacg ggcctgcaaa acaatctggc gctattgctg
    31921 tttagtagcg atcgccgtga cgaaggtttt gccgtcctgg aacagatggc gaaatcgaac
    31981 gccgggcgcg aaggggcctc taaaatctgg tacgggcaga ttaaagacat gcccgtcagt
    32041 gatgccagtg tgtcggcgct gaaaaaatat ctctcgatct ttagtgatgg cgatagcgtg
    32101 gcggctgcgc aatcgcaact ggcagaacag caaaaacagc tggccgatcc tgctttccgc
    32161 gctcgtgcgc aaggtttagc ggcggtggac tctggtatgg cgggtaaagc cattcccgaa
    32221 ctacaacagg cggtgcgggc gaacccgaaa gacagtgaag ctctgggggc gctgggccag
    32281 gcgtattctc agaaaggcga tcgcgccaat gcagtggcga atctggaaaa agccctcgca
    32341 ctggacccgc acagcagcaa caacgacaaa tggaacagtc tgctgaaagt aaaccgctac
    32401 tggctggcga tccagcaggg tgatgctgcg ctgaaagcca ataatcctga ccgggcagaa
    32461 cggttgttcc agcaggcacg taatgtcgat aacaccgaca gctatgcggt gctggggctg
    32521 ggcgatgtgg cgatggcgcg caaagattac cccgccgctg aacgctatta tcagcagacc
    32581 ctgcggatgg acagcggcaa cactaatgcc gtgcgcgggc tggcgaatat ttaccgccag
    32641 cagtcgccag aaaaagctga agcgtttatc gcctcgctct ctgccagcca gcggcgcagc
    32701 attgatgata tcgaacgcag cctgcaaaac ggccgtctgg cacagcaggc agaggtactg
    32761 gaaaaccagg gcaaatgggc gcaggcggca gcacttcagc ggcaacgact ggcgctggac
    32821 cccggcagcg tatggattac ttaccgactt tcgcaggatc tctggcaggc cggacaacgc
    32881 agccaggccg atacgttaat gcgcaatctg gcgcagcaga agccgaacga cccggagcag
    32941 gtttacgctt acgggctgta cctctcaggt cacaaccagg acagagcggc gctggcgcat
    33001 atcaacagcc tgccgcgcgc gcagtggaac agcaatattc aggagctggt taatcgactg
    33061 caaagcgatc aggtgctgga aaccgctaat cgcctgcgag aaagcggcaa agaggctgaa
    33121 gcggaagcga tgctgcgtca gcaaccacct tccacgcgta ttgacctcac gctggctgac
    33181 tgggcgtaac aacgacgtga ttacaccgcc gcccgcactg catatcagaa tgtcctgacg
    33241 cgggagccaa ctaacgccga tgccattctt ggtctgacgg aagtggatat tgctgccggt
    33301 gacaaagcgg cggcacgtag ccagctggcg aaactgcctg ccaccgataa cacctcgctg
    33361 aacactcagc ggcgcgtggc gctggcacag gcgcagcttg gagataccgc agcggcgcag
    33421 cagacgttta ataagttgat cccgcaggca aaatctcagc caccgtcgat ggaaagcgcg
    33481 atggtgctgc gcgacggtgc gaagtttgaa gcgcaggcgg gcgatccaaa gcaggcgctg
    33541 gaaacctaca aagacgccat ggtcgcatcc ggtgtgacta cgacgcgtcc gcaggataac
    33601 gacaccttta cccgactgac ccgtaacgac gagaaagatg actggctgaa acgcggcgtg
    33661 cgcagcgatg cggcggacct ctatcgccag caggatctta acgtcaccct tgagcacgat
    33721 tactggggtt cgagcggcac cggtggttac tccgatctga aagcgcacac taccatgttg
    33781 caggtggatg cgccgtattc tgacgggcgg atgttctttc gcagtgattt cgtcaatatg
    33841 aacgtcggca gtttctccac taatgccgat ggcaaatggg atgacaactg gggcacctgt
    33901 acattacagg actgtagcgg caaccgcagc cagtcggatt ccggtgccag cgtggcggtc
    33961 ggctggcgaa atgacgtctg gagctgggat atcggtacca cgccgatggg cttcaacgtg
    34021 gtggatgtgg tcggcggcat cagttacagc gatgatatcg ggccgctggg ttacaccgtt
    34081 aacgcccacc gtcggcccat ctccagttct ttgctggcct ttggtgggca aaaagactcc
    34141 ccgagcaata ccgggaaaaa atggggtggc gtacgtgccg acggtgtggg gctaagtctg
    34201 agctacgata aaggtgaagc aaacggcgtc tgggcatcgc ttagtggcga ccagttaacc
    34261 ggtaaaaatg tcgaagataa ctggcgcgtg cgctggatga cgggctatta ctataaggtc
    34321 attaaccaga acaatcgccg cgtcacaatc ggcctgaaca acatgatctg gcattacgac
    34381 aaagatctga gtggctactc actcggtcag ggcggttact acagtccgca ggaatacctg
    34441 tcgtttgcca taccggtgat gtggcgggag cgcacggaaa actggtcgtg ggagctgggg
    34501 gcgtctggct cgtggtcgca ttcacgcacc aaaaccatgc cgcgttatcc gctgatgaat
    34561 ctgatcccga ccgactggca ggaagaagct gcgcggcaat ccaacgatgg cggcagcagt
    34621 cagggcttcg gctacacggc gcgggcatta cttgaacgac gtgttacttc caactggttt
    34681 gttggcacgg caattgatat ccagcaggcg aaagattacg cacccagcca ttccctgctc
    34741 tacgtacgtt attccgccgc cggatggcag ggtgacatgg atttaccgcc gcagccgctg
    34801 ataccttacg ccgactggta agttttcaga tagcgcctct cttaatgccg ctgcgatcgg
    34861 gtatactcgg gcggcaatct gggatttccg gggggagaca atttgcgcgt aagtcgctcg
    34921 ttaacaatca agcagatggc aatggtggca gccgttgtcc tggtgttcgt ttttattttt
    34981 tgcaccgttt tgctgttcca tctggtccag cagaatcgct ataacacggc tacgcaactg
    35041 gaaagcattg ctcgctctgt ccgcgaaccc ttatcttcag ctattttgaa aggcgatatt
    35101 cccgaagcgg aagctattct tgccagcatt aaaccggcag gcgtggtcag ccgtgccgat
    35161 gtagtgctgc ctaaccagtt ccaggcgctg cgtaaaagtt ttattccaga gcgtccggtg
    35221 ccggtaatgg ttactcgcct gtttgagcta ccggttcaaa tctcgctggg cgtctactcg
    35281 ctcgaacgtc cggcaaatcc gcagccaata gcctatctgg tgctacaggc ggattccttc
    35341 cgtatgtata agttcgtgat gagcactctc tcaacgttag tgaccattta cttacttttg
    35401 tcgcttatcc tgaccgtcgc catcagctgg tgcattaacc gcctgatttt gcatccgtta
    35461 cgcaatattg ctcgcgaact taacgccatc ccagcccagg agcttgttgg tcaccaactg
    35521 gcattaccgc gtctgcatca ggacgatgaa atcggtatgc tggtgcgcag ttacaacctc
    35581 aaccagcaat tgctgcagcg ccattatgaa gaacagaacg aaaatgcgat gcgcttcccg
    35641 gtgtcggatt tgccgaacaa agccttgctg atggagatgc tggagcaggt tgttgcgcgt
    35701 aaacaaacca ccgcgctgat gatcatcacc tgtgaaaccc tgcgtgatac tgcgggcgtg
    35761 ctgaaagagg cgcaacgaga aattctgctg ctgacgctgg tggaaaaact caaatcggta
    35821 ctgtcgccac gtatgatcct cgcgcagatt agcggttatg actttgctgt cattgccaac
    35881 ggtgtacagg aaccgtggca cgcaatcact ttgggtcagc aagtactcac tatcatgagc
    35941 gagcgcctgc cgattgaacg tattcaactc cgtccgcact gtagcattgg cgtggcgatg
    36001 ttctacggcg atctcaccgc cgaacagctt tacagtcgcg ctatttctgc ggcatttacc
    36061 gctcgccata aaggcaagaa tcagattcag ttctttgatc cgcagcagat ggaagcagcc
    36121 cagaagcggt tgacggaaga gagcgatatc cttaatgcac tggaaaatca tcagtttgca
    36181 atttggttac agccacaggt cgagatgacc agcggtaaac tggtcagtgc ggaagtgtta
    36241 ctgcgtatcc agcaaccgga tggcagttgg gacctgccgg atggcttaat tgatcgcatt
    36301 gagtgctgtg ggctgatggt taccgtcggt cactgggtgc tggaagagtc ctgtcgattg
    36361 cttgcagcct ggcaagagcg cggcattatg ctgcccttgt cggtaaacct ctctgcgctg
    36421 caactgatgc acccgaatat ggtggcggat atgctggaac tgttaacccg ctatcgcatt
    36481 cagccgggaa cactgattct ggaagtgaca gaaagccgac gtattgacga ccctcatgct
    36541 gcggtggcaa tcctccgtcc gctgcgcaat gccggagttc gggtggcgct ggatgatttc
    36601 ggcatgggct acgcagggct gcgtcagctg cagcatatga aatcgttgcc aatcgacgta
    36661 ctgaaaatcg acaaaatgtt tgttgaaggc ttgccggaag atagcagcat gattgctgca
    36721 attatcatgc tggcgcagag cctgaactta caaatgattg ccgaaggcgt ggagactgaa
    36781 gcacaacgag actggctggc aaaagcgggc gttggtattg cccagggctt cctttttgct
    36841 cgcccactcc ctattgaaat cttcgaagag agttacctgg aagaaaagta gctaccccaa
    36901 actgattaca aaactttaaa aagtgctggt ttgtgcgagc cagctcaaac tttttaacct
    36961 ttttgtttca attatgatcc aggtacattt ctgtgatgtt gtctgggtgt tattttaagg
    37021 ccgcaggtac ccccataacc ttacaagacc tgtggtttta ctaaaggaca ccctatgaaa
    37081 acctctctgt ttaaaagcct ttactttcag gtcctgacag cgatagccat tggtattctc
    37141 cttggccatt tctatcctga aataggcgag caaatgaaac cgcttggcga cggcttcgtt
    37201 aagctcatta agatgatcat cgctcctgtc atcttttgta ccgtcgtaac gggcattgcg
    37261 ggcatggaaa gcatgaaggc ggtcggtcgt accggcgcag tcgcactgct ttactttgaa
    37321 attgtcagta ccatcgcgct gattattggt cttatcatcg ttaacgtcgt gcagcctggt
    37381 gccggaatga acgtcgatcc ggcaacgctt gatgcgaaag cggtagaggt ttacgccgat
    37441 caggcgaaag accagggcat tgtcgccttc attatggatg tcatcccggc gagcgtcatt
    37501 ggcgcatttg ccagcggtaa cattctgcag gtgctgctgt ttgccgtact gtttggtttt
    37561 gcgctccacc gtctgggcag caaaggccaa ctgattttta acgtgatcga aagtttctcg
    37621 caggtcatct tcggcatcat caatatgatc atgcgtctgg cacctattgg tgcgttcggg
    37681 gcaatggcgt ttaccatcgg taaatacggc gtcggcacac tggtgcaact ggggcagctg
    37741 attatctgtt tctacattac ctgtatcctg tttgtggtgc tggtattggg ttcaatcgct
    37801 aaagcgactg gtttcagtat cttcaaattt atccgctaca tccgtgaaga actgctgatt
    37861 gtactgggga cttcatcttc cgagtcggcg ctgccgcgta tgctcgacaa gatggagaaa
    37921 ctcggctgcc gtaaatcggt ggtggggctg gtcatcccga caggctactc gtttaacctt
    37981 gatggcacat cgatatacct gacaatggcg gcggtgttta tcgcccaggc cactaacagt
    38041 cagatggata tcgtccacca aatcacgctg ttaatcgtgt tgctgctttc ttctaaaggg
    38101 gcggcagggg taacgggtag tggctttatc gtgctggcgg cgacgctctc tgcggtgggc
    38161 catttgccgg tagcgggtct ggcgctgatc ctcggtatcg accgctttat gtcagaagct
    38221 cgtgcgctaa ctaacctggt cggtaacggc gtagcgacca ttgtcgttgc taagtgggtg
    38281 aaagaactgg accacaaaaa actggacgat gtgctgaata atcgtgcgcc ggatggcaaa
    38341 acgcacgaat tatcctctta atctcaccac ttatgcccgc actcccttcc ccgagtgcgg
    38401 gtgtttgcgc ataattgacc cctgtgcctg aacttaccac ctgggtgagt tcaggtttat
    38461 ttcacttctt ccgtgacatt ttcatgttct tgcggtctaa cacgaagtgt ttttaacgtc
    38521 atttagactg accgacaacg ggcagttttt ttattaccag gatagttgat caggggttca
    38581 catgcagggc ataaaaattc gacttttagc gggcggtttg ctgatgatgg ccactgctgg
    38641 ctatgtgcag gcagatgcgc tccagcctga tccagcatgg caacagggga cgctttccaa
    38701 cggtttacag tggcaagtgc tgaccacccc ccagcgtccc agcgatcgtg ttgaaattcg
    38761 cctgctggtt aataccggtt cgctcgccga aagtacacaa cagagcggtt acagtcacgc
    38821 catccctcgt attgcgctaa cgcaaagcgg tggccttgac gcagcacagg cgcgttcatt
    38881 gtggcagcag gggatcgacc ctaaacgccc gatgccgccg gtaattgtct cttatgacac
    38941 cacgctgttt aatctgagtt tgcccaataa ccgtaacgat ttgctgaaag aagcgctctc
    39001 ttatctggca aatgccacag gcaaattgac catcacacca gaaaccatca accacgcgct
    39061 gcaaagtcag gacatggtgg caacctggcc tgccgatact aaagagggct ggtggcgcta
    39121 tcgtctgaaa gggtcaacct tgttaggtca cgatcctgcc gatccgctga aacaacccgt
    39181 tgaagcggaa aaaattaaag atttctatca gaaatggtac accccggatg caatgacgct
    39241 actggtggtg ggaaacgtgg atgcgcgctc ggttgtcgac caaatcaaca aaacgtttgg
    39301 cgaactgaaa ggcaaacgtg aaacgccagc tccggtgccg acgctttctc cgctgcgtgc
    39361 ggaagcggtg agtattatga ctgacgcggt gcgtcaggac cggttatcta tcatgtggga
    39421 tacgccgtgg cagccgattc gtgaatcagc cgcactgctg cgctactggc gtgcggacct
    39481 ggcccgcgag gcgctgttct ggcatgttca gcaagcgtta agcgccagtg acagcaaaga
    39541 catcggtctt ggatttgact gccgtgtgct gtatctgcgt gcgcagtgtg ccatcaacat
    39601 cgaatcacca aacgacaagc tgaacagcaa ccttaatctg gtggcgcgtg aactggcgaa
    39661 ggttcgcgat aaaggtctgc cggaagaaga gttcaatgcg ttagtggcgc aaaagaaact
    39721 ggagctgcag aaactgtttg ccgcctatgc acgggctgat accgatattc tgatgggcca
    39781 gcggatgcgt tcgttgcaaa atcaggtcgt cgatatcgcg ccggagcagt atcagaaact
    39841 gcggcaggat ttccttaaca gcctgactgt ggagatgtta aatcaggatc tgcgtcagca
    39901 gttgtcgaat gatatggcgt taatactgct gcagccgaaa ggcgagccgg aatttaacat
    39961 gaaagcgttg caggcggcct gggatcaaat catggcccca tctaccgccg ctgcgaccac
    40021 ctctgtcgcc acggatgacg tacatcctga agtgacggat attccacctg cacagtaatg
    40081 cctgatggtg atgagttagc acaaccatca ggtatttatg gatgagctgg atagaggggt
    40141 aacactttat ccagcctttt gcatatgctg cgtttacgct ggcatcgcct cacgcgggat
    40201 aatcgcgccg cgatactgaa taacggtact tgcggtcagg tgcccgcgtt tcgccgcgtt
    40261 ttccgcactg ccgcccgtca aacgtaccgc cagataaccg gcgctgaaag agtcgccagc
    40321 tgcggtggta tcgatcactt tttctttcgg cagtttcacc gccggaacat caactaacgc
    40381 ttcaccagca atggacacca ggcaagaatc cgccccgcgt ttcaccacca cttctttcac
    40441 gcccgcgtta tgggtgcgcg caatgacgtc ttccaccggc tgttgacccc acagcgcgtc
    40501 ttcgtcgtcc agcgtcagga aggcgatatc cgtgcattcc agcatttgct ggtacacctg
    40561 ctgtgtctct tctttgctgg cccacaggcg cggacgatag ttattgtcga aaatcacttt
    40621 tccgcctttg gcgcggcatt cgtgcagcag ggaaagcagc ttttcgcggc tggtcgggct
    40681 taagatcgcc aggctaatcc cgctcaggta gagataatcg aaattcgcca gctcttcgca
    40741 aatcgccgca gactgctcgc tcgccagcca gaatttggcg gcggcttcgt tacgccagta
    40801 gtagaacgta cgttcgccgg tgctgtcggt ttcaatgtag taaaggcccg gaagacggtt
    40861 ttccatccgt tgggtcaggg aagtatcaac gttctcgccg tgccaggcgt ccagcatctg
    40921 ctggctaaaa ctgtccgttc ccagcgccgt tacgtaatga acggttaatg ccgcaggatc
    40981 gacctgacgg gcgatataga cggaagtgtt cagggtatcg ccgccgaaac cgcgcttaac
    41041 gtccgcgcct ttctcggaaa gctcaatcat gcattcgcca atcacggcaa tctttttgga
    41101 catagtcgtg aacctgatct gtaaaaaatt agcgttagtt tgcgctgtgg tggtttgctg
    41161 gtcaaccata ttaaaacagt gttccattat ttttttgagc cagaacgtat tcctgaaaga
    41221 ttccgttgtg gagtgaggaa atttaccccg gccaggcggt aaagttctgc ccttacgcgc
    41281 cgataatctt tgtcgagtcc gggcagcatc acttttaaac acaggacatc tttgatgata
    41341 aggcaggtta tccagcgaat aagcaaccct gaagcaagca tcgagagctt gcaggaacgg
    41401 cgtttttggt tgcagtgtga gcgtgcttac acctggcagc cgatctatca aacatgcggg
    41461 cggttaatgg ccgtggagct attaacggtg gtcacgcatc ccttgaaccc ttcgcaactc
    41521 ctgccgccgg atcgctattt tactgaaatc accgtcagcc atcggatgga ggttgtgaaa
    41581 gagcagattg atttgctggc gcaaaaagcc gacttctttg tagagcacgg cctgctggca
    41641 tcggtcaata ttgatggccc tacgctcatc gccctgcgtc agcaaccaaa aatcctgcgc
    41701 cagattgagc gtcttccctg gctgcgtttc gaactggtgg agcatatccg tctgccgaaa
    41761 gattcaacct ttgcctcgat gtgtgaattt ggcccgctgt ggctggatga ttttggtacc
    41821 gggatggcaa atttctctgc actaagtgaa gtgcgttatg actacatcaa aatcgcgcga
    41881 gaactgtttg tgatgctgcg tcagtcgccg gaaggccgca cactcttttc tcagctttta
    41941 catctaatga atcgctattg tcgcggggtg attgtcgagg gcgtagaaac accggaagag
    42001 tggcgtgatg ttcagaactc gcccgcattc gccgcacaag gctggtttct ttcacgcccg
    42061 gcaccgatgg aaacgctgaa tacggcggtt ctggcgctat aagctgcctc attttccgcc
    42121 gggctggact atctttagga ctggcacagg aagaatatga ggaagcgaac gatgagcaag
    42181 gcaggcaaaa taaccgctgc gatttcaggg gctttcttgt tgttgattgt cgtggcgatc
    42241 attttgattg caacttttga ctggaatcga ctcaaaccga ccatcaacca gaaagtctct
    42301 gcggagttga atcgtccgtt cgctatccgt ggcgatctgg gcgtggtgtg ggagcggcaa
    42361 aaacaagaaa ctggctggcg cagctgggtg ccgtggcctc atgtacatgc agaagacatc
    42421 attcttggta atccaccgga tattcccgaa gtcacgatgg tgcatttgcc gcgagtagag
    42481 gcaacgctga caccgctggc gctgctgacc aaaacggtct ggctgccgtg gatcaagctc
    42541 gaaaagcccg acgcgcgcct gattcgcctc tctgaaaaga acaataactg gacgtttaat
    42601 cttgccaacg atgacaacaa agacgcgaat gcaaagccgt cggcatggtc gtttcggctg
    42661 gataatattc ttttcgatca agggcggatc gccattgatg acaaagtaag caaagcggat
    42721 ctggaaattt ttgttgatcc gttaggcaag ccgctgccat tcagcgaagt tactggatcg
    42781 aaaggtaaag cggataaaga aaaggtgggc gattacattt ttggcctgaa ggcgcaggga
    42841 cgttataacg gtgaaccgct cacgggtacg ggaaaaatag gcggtatgct ggcgctgcgt
    42901 ggcgaaggga cgccgtttcc ggtacaggct gatttccgtt caggtaatac ccgtgttgct
    42961 tttgatggcg tcgtgaatga cccaatgaag atgggcggtg tcgatttacg gcttaaattt
    43021 tctggcgatt cactgggtga tctctatgaa ctgacgggcg ttctgctgcc cgataccccg
    43081 ccgtttgaaa cggatggtcg gctggtagcg aaaatcgaca ctgaaaaatc gtcggtcttt
    43141 gattatcgcg gttttaatgg gcgaattggc gatagcgata tccacggttc tctggtctac
    43201 accaccggaa agcaacgacc aaaactggaa ggtgatgtcg agtcgcggca attgcggctg
    43261 gcggacctgg gaccgttgat tggcgttgat tccgggaaag gggcagaaaa gtcgaaacgg
    43321 tctgaacaga agaagggcga aaaaagcgtt cagcctgcgg gcaaagtgct gccttatgac
    43381 cgcttcgaaa ccgataaatg ggacgttatg gatgccgatg ttcgcttcaa agggcggcgc
    43441 attgagcatg gcagtagcct gccgattagc gatctttcta ctcatatcat cctcaaaaat
    43501 gctgacctgc gcctgcaacc gctgaaattt ggcatggcgg gcggcagcat tgcggcgaat
    43561 attcatctgg aaggcgataa aaagccgatg caggggcggg cagatattca ggctcgtcga
    43621 ctgaaactga aagaactgat gcccgatgtg gaactgatgc agaagacgct gggggaaatg
    43681 aacggtgacg cggaactacg cggtagcggt aactcggtgg cggcgctttt aggcaacagt
    43741 aacggcaacc tgaaactgtt gatgaatgac gggctggtga gccgcaacct gatggagatt
    43801 gttgggctga atgtcggcaa ctacattgtc ggtgcgatat ttggtgacga tgaggtgcgg
    43861 gtgaactgtg cggcggcgaa tctgaatatt gccaacggcg tggcgcgccc gcagattttt
    43921 gctttcgata ctgagaacgc gttgattaat gttatcggca cggcaagttt tgcttcggaa
    43981 cagctggatt tgactattga tccggagagt aaagggattc ggattatcac actgcgttcg
    44041 ccgctgtatg tgcgtgggac gtttaaaaat ccgcaggctg gggtgaaagc cggaccgctg
    44101 attgcccgtg gtgctgttgc tgcggcactg gcaacgctgg taacaccggc ggcggcgtta
    44161 ctggcactga tctcaccttc cgaaggggag gctaatcagt gtcggacgat attgtcgcag
    44221 atgaagaagt gatgataatt gccggatgtc tgacatccgg caattaaaca gatttagatt
    44281 tttggcaaag tgtgctttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
    44341 atccttgtga attcaatata ttgtatggaa attcagtcct gataagcgta gcgcatcagg
    44401 cttttcacac ttattacaac gactgatgtc gcgtctcatg ggtcagcagc agggcgatta
    44461 acgtcaagcc agccatcgcc gccagatata accccaccgc acctaacccg tagttagcct
    44521 gcaaccaggt tgcgatatac ggcgcaacgg aagccccgag aatcgacgat acgttgtaag
    44581 agaacgatgc tccggtgtaa cgcacttctg tcggaaacag ctctggtaac agcgcaccca
    44641 tcggcccgaa ggtcagcccc atcagactta accccagcag caggaaggca aaaaccagaa
    44701 tcgggttgcc agaaccgagc agcgggttaa aggcgaacag cgcgaacagg atgatcagcg
    44761 tggtgatgat caccatgctt ttacggcgac caaaggcatc agccagtaat ccggcgactg
    44821 gcaccatcac gccaaaacca ataactgcca tcatcagcat ccacaacact tcgttacgcg
    44881 gcaggccaag cccaactggc gcggcggcgg tactgaaggt catagagtag accgtcatga
    44941 tgtaaaacag cgtataggtt gccagcataa tgaacgtacc cagtacggtt acgcgaacat
    45001 gtttggtcag cagcgtaccc agcgggatct tcacctgttt tttcgcttta gcgactttct
    45061 caaacaccgg cgactcatgc agcgacacgc gaacatacag gccgataatg accagcaccg
    45121 ccgagaagat aaatggcaca cgccagcccc agctcataaa ctgctcatcg gtcagtagcc
    45181 aggaaagcag caagaaagtg ccgttggcaa agaagaagcc aatcggtgcg cctagctgtg
    45241 ggaaggagcc atacagtgca cgtttgcgcg gtggggcgtt ttcagtcgcc agcagcgccg
    45301 cgccgcccca ttcaccgcct aagcccagac cctgaccaaa tcgagccagc gccagcagca
    45361 gtggggcgaa aataccaatc gtggcatagc ccggcagcag accaatcacc acggtcgaaa
    45421 tccccatcgt tagcaacgag gcgaccagcg tcgctttacg cccaacgcga tcgccaaaat
    45481 gaccaaaaac ggcagagcca atggggcgcg cgacgaaggc gatggcgaag gtggcgagcg
    45541 actgtagcgt tgctgccgtt ggatcgccct gcgggaagaa gatatgcgga aacacaataa
    45601 cggccgcagt ggcgtaaatg taaaagtcga agaactcaat ggccgtgcca atgagagagg
    45661 cgacaaggac tttattacgc gagttgatcg gcgtgtattc ttgttcgtgg tcgagtgttg
    45721 tggctgttgc ttgcataatc ttttcttatt ttttggctaa cgaatagcca tattacgcac
    45781 agcaaaagtg atatttcaat gtgtaacaaa tcgcggaaat ggtcaaaaac caggcaaaaa
    45841 ggggataatg tttaggccag tgatttttct tctatgaaat gcttcacata attttataaa
    45901 tagtggataa aactggtttc aggttaaata aaagttacca actggattta tatgctgggg
    45961 ttatgagttg gagtgaaatt gtttgctccc aaatagatgg ctgaagaggc tctggggaag
    46021 agtttcaaga ggttatgtgc gcattatagc cggatgcgat gctggcgcat cttatccggc
    46081 ctacggggag ccaggccgaa taaaatcgta tgccggataa ggcgtttacg ccgcatccgg
    46141 cctattttaa ggctgcgttt tccctggcat tcgcgggtcg tctttatatt cggcggtggc
    46201 aatccacgcc gcgcaaaaca gcgtcaaacg ggcgaagaag tagaaaaatg ccatcagccc
    46261 cagcacggaa ccaaatgctg cgccagaggg ggatttcatc agcgatggca gggtgtaggt
    46321 catcacgatt ttaatcactt caaaaccaat agcagccaga aatgttccgc gaatcagggc
    46381 ttttttgcgc ggacggtggc gcggcaaccg ccagaagatc cagaagaaaa gcagatagtt
    46441 ggcgaagatg gaaatcgcca ggccaatcaa tcgccacgtc ggtttcagcc actcaatgct
    46501 gttcaggtgc agggcgctaa taatcatttg ctgcgccgaa ccggcaaccg aggtgatcga
    46561 aagcgtcaca atcagcgcaa tcaacaaacc aatcagtgaa ataaaatcac gcagatattt
    46621 tacccagaac ttctcctgat cttgcggcga gcgttcccag acatcgcgcg actgggcacg
    46681 aatcgcttca cgcaggttac ccatccagtt gatgccggaa taaagtgcca ccgccaggcc
    46741 gacaagccct acagtcgtac gctgctgaac ggcggtgttg atggtgtttt tcaacgtggc
    46801 ggctagtgtc ggatcgctga tgttttgcag aattttgtcg aagatatcct gtagcaacat
    46861 cggatgggag gccagcacaa agccccctgc ggcaaacgac accatcaaaa tcgggatcat
    46921 cgacaaaaat gagaaatagg tgatagccgc accaaactgg ttacccagcc gatcgttaaa
    46981 gcgttctgtc gcgcgaataa ggtgcgcaat aaccggttgg cgttggactt tttcggcagt
    47041 ggtggtgacg gtttccagcg cctggctaac tttgccgcct ttctcgctat tatccaactg
    47101 cttaatcggc tcgtgctcca gatcctgggt gggacgtttg atctcgtttt cctgcgtcat
    47161 ccgctctttt gtccttctgt cgtggtcatt cttaaaaagt atagtcggtc agtccaggtg
    47221 ctctttcatt acgccagcca gccactgcat aaacaggttt acacgccggg aaagctcccg
    47281 acgctgcggg taaaccagcg aaagggagag cggctcggca cggtagcctg gcaacacttc
    47341 aataagccgc ccggcacgca gggcttcgcg cacggcaatg cgcgggatct gaataatccc
    47401 cagaccggca agaccggcgg cgagatagtt ttcgctactg tttaccgtca acatgccgcc
    47461 agacttaaac cactggacgc cattaacgct ggcaacctca aaacctaacg gatgtacacc
    47521 caggtgcggt gtataacgca ctattgcgtg tgaagtcaga tcatcggggc tttgcggata
    47581 accaaagcgc gtcagatagt gcggactggc acagttgacc atggttagtt tgccgagggg
    47641 acgggcgata acgccatctt ccggtaacgc gccagtgcgt atcacgcaat caaaaccatc
    47701 atgaagaata tctaccggac ggttatggct actcagttcc agctcaattc ccggatgctg
    47761 atagagaaat tccgacagac gcggcagtaa caggtttttc gcgattcctg acgggatgtc
    47821 gatgcgtaat ttaccgctga tactggtggc atcctgttga aacagaccgt ccagttcgct
    47881 gagattactc aacacatctt ttgcccgttg ataataggtc atgccttctg gcgtgagttt
    47941 gacccgtcgc gtagtgcgct ggagaagctg ggtgccaagt tgatgttcca gtgcctgtat
    48001 ctggcgcgaa acacttccct ttggcaaagc aaagaaatcc gccgcgcggg aaaaactttc
    48061 cagctccgcg actttgatga acaactgcat tgcgtgaatt ttatccattt ctttgaccga
    48121 ttgttgttca cagcgaaaca gtaacgcgca tatcgcattg tttattgttt tttctaacat
    48181 ctaatgccgg gtttcacaat gttgtcatga cgaatgtggc gatttctcag caggaaaagg
    48241 gcggaatagc tttatgagaa aggtgaatcc ccttaaacag agcgtatttt tcagtgattc
    48301 cctttaaagt gaatagagga cgtattggct atttgtgctt atttttgtct cattgccatt
    48361 tgttattttg gcttgtagtc gcggaatttc atttttaatt gcatgcgtac taaccaccaa
    48421 tataaggaaa tcctcaaaat tgttttaaat agtgccctcg acatgtaatt tcaggcgttt
    48481 agctgtaact atttatatcg gtaactattt tctacgcgtc atctctgtag aatggccacc
    48541 gccaaatggc gaattcttat tttttctcgc gggtaatacc cttccactac ttaagtcttt
    48601 ttaacgtatt caggtggaga ttcagttatg caaatagtca tgtttgacag gcagtcaata
    48661 tttattcatg gaatgaaaat cagtttacag cagcgtattc caggagtgag tattcagggg
    48721 gtcagtcagg cagacgagtt atggcaaaag ctggaaagtt accctgaagc cttagttatg
    48781 ctcgatggcg atcaggatgg tgagttttgc tactggttgc tgcaaaaaac cgtggtgcag
    48841 tttcctgagg ttaaggtgtt aatcacggcg acggattgca ataaacggtg gttacaggaa
    48901 gttatccatt ttaatgtgct ggccattgtg cctcgcgatt caaccgtcga gacatttgcg
    48961 ttggtggtaa atagtgcggc gatggggatg atgtttctgc ctggagactg gcggacgacg
    49021 ccggaaaagg acattaaaga tctcaaatcg ttaagcgccc gccagcggga gattttaacc
    49081 atgttagcgg cgggcgaatc aaataaggag atcggcagag cgctgaatat cagtaccgga
    49141 acggtaaaag cgcatctgga atccctgtat cgccgtctgg aagtgaaaaa tcgcacccag
    49201 gcggcaatga tgttaaatat ctcctcctg
//
LOCUS       DBKEKI010000014        48129 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.014, whole genome shotgun sequence.
ACCESSION   DBKEKI010000014 DBKEKI010000000
VERSION     DBKEKI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.014"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            482..2410
                     /gene="thrS"
                     /locus_tag="ACUQPX_000764"
     CDS             482..2410
                     /gene="thrS"
                     /locus_tag="ACUQPX_000764"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HHV9055549.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2414..2956
                     /gene="infC"
                     /locus_tag="ACUQPX_000765"
     CDS             2414..2956
                     /gene="infC"
                     /locus_tag="ACUQPX_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HHV9055550.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3053..3250
                     /gene="rpmI"
                     /locus_tag="ACUQPX_000766"
     CDS             3053..3250
                     /gene="rpmI"
                     /locus_tag="ACUQPX_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HHV9055551.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3303..3659
                     /gene="rplT"
                     /locus_tag="ACUQPX_000767"
     CDS             3303..3659
                     /gene="rplT"
                     /locus_tag="ACUQPX_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HHV9055552.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3776..3900
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3782..3826
                     /gene="pheM"
                     /locus_tag="ACUQPX_000768"
     CDS             3782..3826
                     /gene="pheM"
                     /locus_tag="ACUQPX_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HHV9055553.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4109..5092
                     /gene="pheS"
                     /locus_tag="ACUQPX_000769"
     CDS             4109..5092
                     /gene="pheS"
                     /locus_tag="ACUQPX_000769"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HHV9055554.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPSHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5107..7494
                     /gene="pheT"
                     /locus_tag="ACUQPX_000770"
     CDS             5107..7494
                     /gene="pheT"
                     /locus_tag="ACUQPX_000770"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HHV9055555.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQTSLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7499..7798
                     /gene="ihfA"
                     /locus_tag="ACUQPX_000771"
     CDS             7499..7798
                     /gene="ihfA"
                     /locus_tag="ACUQPX_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HHV9055556.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7899..8879
                     /gene="btuC"
                     /locus_tag="ACUQPX_000772"
     CDS             7899..8879
                     /gene="btuC"
                     /locus_tag="ACUQPX_000772"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HHV9055557.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQVLFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8942..9493
                     /gene="btuE"
                     /locus_tag="ACUQPX_000773"
     CDS             8942..9493
                     /gene="btuE"
                     /locus_tag="ACUQPX_000773"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HHV9055558.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9493..10242
                     /gene="btuD"
                     /locus_tag="ACUQPX_000774"
     CDS             9493..10242
                     /gene="btuD"
                     /locus_tag="ACUQPX_000774"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HHV9055559.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSVLDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10320..10784
                     /locus_tag="ACUQPX_000775"
     CDS             10320..10784
                     /locus_tag="ACUQPX_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HHV9055560.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11031..11744
                     /gene="ydiV"
                     /locus_tag="ACUQPX_000776"
     CDS             11031..11744
                     /gene="ydiV"
                     /locus_tag="ACUQPX_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HHV9055561.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            11808..13244
                     /gene="selO"
                     /locus_tag="ACUQPX_000777"
     CDS             11808..13244
                     /gene="selO"
                     /locus_tag="ACUQPX_000777"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HHV9055562.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13248..13439)
                     /gene="hemP"
                     /locus_tag="ACUQPX_000778"
     CDS             complement(13248..13439)
                     /gene="hemP"
                     /locus_tag="ACUQPX_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HHV9055563.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13571..14617)
                     /gene="aroH"
                     /locus_tag="ACUQPX_000779"
     CDS             complement(13571..14617)
                     /gene="aroH"
                     /locus_tag="ACUQPX_000779"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HHV9055564.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(14774..15607)
                     /gene="ppsR"
                     /locus_tag="ACUQPX_000780"
     CDS             complement(14774..15607)
                     /gene="ppsR"
                     /locus_tag="ACUQPX_000780"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HHV9055565.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            15940..18318
                     /gene="ppsA"
                     /locus_tag="ACUQPX_000781"
     CDS             15940..18318
                     /gene="ppsA"
                     /locus_tag="ACUQPX_000781"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HHV9055566.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18375..20021)
                     /gene="fadK"
                     /locus_tag="ACUQPX_000782"
                     /pseudo
     CDS             complement(18375..20021)
                     /gene="fadK"
                     /locus_tag="ACUQPX_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20078..20371)
                     /locus_tag="ACUQPX_000783"
     CDS             complement(20078..20371)
                     /locus_tag="ACUQPX_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HHV9055567.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20368..21657)
                     /locus_tag="ACUQPX_000784"
     CDS             complement(20368..21657)
                     /locus_tag="ACUQPX_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HHV9055568.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21713..22651)
                     /locus_tag="ACUQPX_000785"
     CDS             complement(21713..22651)
                     /locus_tag="ACUQPX_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HHV9055569.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYDPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22671..23435)
                     /locus_tag="ACUQPX_000786"
     CDS             complement(22671..23435)
                     /locus_tag="ACUQPX_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HHV9055570.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            23751..24662
                     /locus_tag="ACUQPX_000787"
     CDS             23751..24662
                     /locus_tag="ACUQPX_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HHV9055571.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(24705..25856)
                     /locus_tag="ACUQPX_000788"
     CDS             complement(24705..25856)
                     /locus_tag="ACUQPX_000788"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HHV9055572.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYSRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(25870..27465)
                     /locus_tag="ACUQPX_000789"
     CDS             complement(25870..27465)
                     /locus_tag="ACUQPX_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HHV9055573.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLIIALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYAIFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTASSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFRGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(27611..28369)
                     /gene="aroD"
                     /locus_tag="ACUQPX_000790"
     CDS             complement(27611..28369)
                     /gene="aroD"
                     /locus_tag="ACUQPX_000790"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HHV9055574.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(28400..29266)
                     /gene="ydiB"
                     /locus_tag="ACUQPX_000791"
     CDS             complement(28400..29266)
                     /gene="ydiB"
                     /locus_tag="ACUQPX_000791"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HHV9055575.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(29278..30543)
                     /locus_tag="ACUQPX_000792"
     CDS             complement(29278..30543)
                     /locus_tag="ACUQPX_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9055576.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(30770..31984)
                     /locus_tag="ACUQPX_000793"
     CDS             complement(30770..31984)
                     /locus_tag="ACUQPX_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9055577.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(32084..32440)
                     /locus_tag="ACUQPX_000794"
     CDS             complement(32084..32440)
                     /locus_tag="ACUQPX_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HHV9055578.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(32576..32683)
                     /gene="rprA"
                     /locus_tag="ACUQPX_000795"
     ncRNA           complement(32576..32683)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACUQPX_000795"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(32869..33981)
                     /gene="ydiK"
                     /locus_tag="ACUQPX_000796"
     CDS             complement(32869..33981)
                     /gene="ydiK"
                     /locus_tag="ACUQPX_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HHV9055579.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            34370..37426
                     /gene="ydiJ"
                     /locus_tag="ACUQPX_000797"
     CDS             34370..37426
                     /gene="ydiJ"
                     /locus_tag="ACUQPX_000797"
                     /EC_number="1.1.99.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1903457 - lactate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyglutarate dehydrogenase YdiJ"
                     /protein_id="HHV9055580.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            37423..37833
                     /gene="menI"
                     /locus_tag="ACUQPX_000798"
     CDS             37423..37833
                     /gene="menI"
                     /locus_tag="ACUQPX_000798"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HHV9055581.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            37933..38121
                     /locus_tag="ACUQPX_000799"
     CDS             37933..38121
                     /locus_tag="ACUQPX_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HHV9055582.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            38669..39037
                     /gene="sufA"
                     /locus_tag="ACUQPX_000800"
     CDS             38669..39037
                     /gene="sufA"
                     /locus_tag="ACUQPX_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HHV9055583.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            39046..40533
                     /gene="sufB"
                     /locus_tag="ACUQPX_000801"
     CDS             39046..40533
                     /gene="sufB"
                     /locus_tag="ACUQPX_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HHV9055584.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKSVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            40543..41289
                     /gene="sufC"
                     /locus_tag="ACUQPX_000802"
     CDS             40543..41289
                     /gene="sufC"
                     /locus_tag="ACUQPX_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HHV9055585.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            41264..42535
                     /gene="sufD"
                     /locus_tag="ACUQPX_000803"
     CDS             41264..42535
                     /gene="sufD"
                     /locus_tag="ACUQPX_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HHV9055586.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTESLRDEGLKQQVLARIGQRLPGGAR"
     gene            42532..43752
                     /gene="sufS"
                     /locus_tag="ACUQPX_000804"
     CDS             42532..43752
                     /gene="sufS"
                     /locus_tag="ACUQPX_000804"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HHV9055587.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            43765..44181
                     /gene="sufE"
                     /locus_tag="ACUQPX_000805"
     CDS             43765..44181
                     /gene="sufE"
                     /locus_tag="ACUQPX_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HHV9055588.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            44330..45334
                     /gene="ldtE"
                     /locus_tag="ACUQPX_000806"
     CDS             44330..45334
                     /gene="ldtE"
                     /locus_tag="ACUQPX_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HHV9055589.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(45398..45634)
                     /gene="lpp"
                     /locus_tag="ACUQPX_000807"
     CDS             complement(45398..45634)
                     /gene="lpp"
                     /locus_tag="ACUQPX_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HHV9055590.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAVRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(45945..47357)
                     /gene="pykF"
                     /locus_tag="ACUQPX_000808"
     CDS             complement(45945..47357)
                     /gene="pykF"
                     /locus_tag="ACUQPX_000808"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HHV9055591.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(47365..47559)
                     /locus_tag="ACUQPX_000809"
     CDS             complement(47365..47559)
                     /locus_tag="ACUQPX_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055592.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            47914..48123
                     /gene="fumD"
                     /locus_tag="ACUQPX_000810"
     CDS             47914..48123
                     /gene="fumD"
                     /locus_tag="ACUQPX_000810"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HHV9055593.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT        12218 a        11646 c        12388 g        11877 t
ORIGIN      
        1 gattaattat tatcgccaga acgaaatgtg cacgaaaaat tgttataaga attgtgtcac
       61 ttttatcatt gtgatatgac cacttcgtca ttaatctcat gagcgtttaa aatactaccg
      121 ccaaatcacg caacgaactt tgctggattc agtttatgct gctgtaaatc cgctcgcagt
      181 aaacctttca gacgcaccgt gatgttatca gtggttcttt aagcgttttg ctggtgtact
      241 cactacaaac gaattgcgaa tcgaatcaat gtgaaacgga aaggtacaat ctcccttttt
      301 ttagtgttaa catcgttcaa ccgggtggaa ggtgagaggt taatgtcttt catttgtgta
      361 cctgattgcg aaccaattta gcatttgttg gctagatggt ttcgcaatga actgttaata
      421 aacaaatttt tctttgtatg tgatctttcg tgtgggtcac cactgcaaat aaggatataa
      481 aatgcctgtt ataactcttc ctgatggcag ccaacgccat tacgaccacg ctgtaagccc
      541 catggatgtt gcgctggaca ttggtccagg tctggcgaaa gcctgtatcg cagggcgcgt
      601 taatggcgaa ctggttgatg cttgcgatct gattgaaaac gacgcacaac tgtcgatcat
      661 taccgccaaa gacgaagaag gtctggagat cattcgtcac tcctgtgcgc acctgttagg
      721 gcatgcgatt aaacaacttt ggccgcatac caaaatggca atcggcccgg ttattgacaa
      781 cggtttttat tacgacgttg atcttgaccg cacgttaacc caggaagatg tcgaagcact
      841 cgagaagcgg atgcatgagc ttgctgagaa aaactacgac gtcattaaga agaaagtcag
      901 ctggcacgaa gcgcgtgaaa ctttcgccaa ccgtggggag agctacaaag tctccattct
      961 tgacgaaaac atcgcccatg atgacaagcc aggtctgtac ttccatgaag aatatgtcga
     1021 tatgtgccgc ggtccgcacg taccgaacat gcgtttctgc catcatttca aactaatgaa
     1081 aacggcaggg gcttactggc gtggcgacag caacaacaaa atgttgcaac gtatttacgg
     1141 tacggcgtgg gcagacaaaa aagcacttaa cgcttacctg cagcgcctgg aagaagccgc
     1201 gaaacgcgac caccgtaaaa tcggtaaaca gctcgacctg taccatatgc aggaagaagc
     1261 gccgggtatg gtattctggc acaacgacgg ctggaccatc ttccgtgaac tggaagtgtt
     1321 tgttcgttct aaactgaaag agtaccagta tcaggaagtt aaaggtccgt tcatgatgga
     1381 ccgtgtcctg tgggaaaaaa ccggtcactg ggacaactac aaagatgcaa tgttcaccac
     1441 atcttctgag aaccgtgaat actgcattaa gccgatgaac tgcccgggtc acgtacaaat
     1501 tttcaaccag gggctgaagt cttatcgcga tctgccgctg cgtatggccg agtttggtag
     1561 ctgccaccgt aacgagccgt caggttcgct gcatggcctg atgcgcgtgc gtggatttac
     1621 ccaggatgac gcgcatatct tctgtactga agaacaaatt cgcgatgaag ttaacggatg
     1681 tatccgttta gtctatgata tgtacagcac ttttggcttc gagaagatcg tcgtcaaact
     1741 ctccactcgt cctgaaaaac gtattggcag cgacgaaatg tgggatcgtg ctgaggcgga
     1801 cctggcggtt gcgctggaag aaaacaacat cccgtttgaa tatcaactgg gtgaaggcgc
     1861 tttctacggt ccgaaaattg aatttaccct gtatgactgc ctcgatcgtg catggcagtg
     1921 cggtacagta cagctggact tctccttgcc gtctcgtctg agcgcctcct atgtgggcga
     1981 agacaacgag cgtaaggtac cggtaatgat tcaccgcgca attctggggt cgatggagcg
     2041 tttcatcggt atcctgaccg aagaattcgc tggtttcttc ccgacctggc ttgcgccagt
     2101 tcaggttgtt atcatgaata ttaccgattc acagtctgaa tacgttaacg aattgacgca
     2161 aaaactatca aatgcgggca ttcgtgttaa agcagacttg agaaatgaga agattggctt
     2221 taaaatccgc gagcacactt tgcgtcgcgt cccatatatg ctggtctgtg gtgataaaga
     2281 ggtggaatca ggcaaagttg ccgttcgcac ccgccgtggt aaagacctgg gaagcatgga
     2341 cgtaaatgaa gtgatcgaga agctgcaaca agagattcgc agccgcagtc ttaaacaatt
     2401 ggaggaataa ggtattaaag gcggaaaacg agttcaaacg gcgcgcccta accgtatcaa
     2461 tggcgaaatt cgcgcccagg aagttcgctt aacaggtctg gaaggcgagc agcttggtat
     2521 tgtgagtctg agagaagctc tggagaaagc agaagaagcc ggagtagact tagtcgagat
     2581 cagccctaac gccgagccgc cggtttgtcg tataatggat tacggcaaat tcctctatga
     2641 aaagagcaag tcttctaagg aacagaagaa aaagcaaaaa gttatccagg ttaaggaaat
     2701 taaattccgt cctggtacag atgaaggcga ctatcaggta aaactccgca gcctgattcg
     2761 ctttctcgaa gagggtgata aagccaaaat cacgctgcgt ttccgcggtc gtgagatggc
     2821 gcaccagcaa atcggtatgg aagtgcttaa tcgcgtgaaa gacgatttgc aagaactggc
     2881 agtagtcgaa tccttcccaa cgaagatcga aggccgccag atgatcatgg tgctcgctcc
     2941 taagaagaaa cagtaaggcc ttcaagtagc actgtctgtg gagcctttgg gcttcacagg
     3001 cattgttcgc ctttgtttcg tttattaaca atgcgaagtg gaagttatta aaatgccaaa
     3061 aattaagacc gtacgcggtg ctgctaagcg cttcaaaaaa accggtaaag gtggttttaa
     3121 gcacaagcac gctaacctgc gtcacattct gactaaaaaa gcgaccaaac gtaaacgtca
     3181 cctgcgtccg aaagccatgg tttccaaagg cgatctgggc ctggtaatcg cgtgcctgcc
     3241 gtacgcataa gccgttaacg tttttaactt tttaattaga atatagatac aggagagcac
     3301 atatggctcg cgtaaaacgt ggtgttattg cacgcgcacg tcacaagaaa attttgaaac
     3361 aagctaaagg ctactacggt gcgcgttctc gcgtataccg cgttgccttc caggctgtta
     3421 tcaaagctgg tcagtatgct taccgtgacc gtcgtcaacg taagcgtcag ttccgtcaac
     3481 tgtggattgc gcgtatcaac gcagcagcac gtcagaacgg tatttcttac agcaaattca
     3541 tcaatggcct gaaaaaagcc tctgttgaaa tcgaccgtaa gatcctggct gatatcgcag
     3601 tattcgacaa agtagcgttc accgctctgg ttgaaaaagc gaaagcagct ctggcataag
     3661 ccagttgaaa gagggagcta gtctccctct tttcgtttca acgccatcaa aacattgact
     3721 tttatcgccg tagccttttc aataaaggtc ttttgaagag taccaaaagg taacgcaagc
     3781 aatgaatgct gctattttcc gcttcttttt ttactttagc acctgaatcc aggaggctag
     3841 cgcgtgagaa gagaaacgga aaacagcgcc tgaaagcctc ccagtggagg ctttttttgt
     3901 atgcgcgttt tgaaaaataa aaagccagtc tatcaggcgt aattgtcgca gccagtctgg
     3961 acacggccag tgcggagaaa ccggagcgta cttaagtacg tgaggatttc gagcactgcc
     4021 cgggaccaaa atggcaagta aaatagcctg atgggatagg ctctaagtcc aacgaaccag
     4081 tgtcaccact gacacaatga ggaaaaccat gtcacatctc gcagaactgg ttgccagtgc
     4141 gaaggcggcc attagccagg cgtcagatgt tgccgcgtta gataatgtgc gcgtcgaata
     4201 tttgggtaaa aaagggcact taacccttca gatgacgacc ctgcgtgagc tgccgccaga
     4261 agagcgtccg gcagctggtg cggttatcaa cgaagcgaaa gagcaggttc agcaggcgct
     4321 gaatgcgcgt aaagcggaac tggaaagcgc tgcactgaat gcgcgtctgg cggcggaaac
     4381 gattgatgtc tctctgccag gtcgtcgcat tgaaaacggc ggtctgcatc cggttacccg
     4441 taccatcgac cgtatcgaaa gtttcttcgg tgagcttggc tttaccgtgg caaccgggcc
     4501 ggaaatcgaa gacgattatc ataacttcga tgctctgaac attcctagtc accacccggc
     4561 gcgcgctgac cacgacactt tctggtttga cgctacccgc ctgctgcgta cccagacctc
     4621 tggcgtacag atccgcacca tgaaagccca gcagccaccg attcgtatca tcgcgcctgg
     4681 ccgtgtttat cgtaacgact acgaccagac tcacacgccg atgttccatc agatggaagg
     4741 tctgattgtt gataccaaca tcagctttac caacctgaaa ggcacgctgc acgacttcct
     4801 gcgtaacttc tttgaggaag atttgcagat tcgcttccgt ccttcctact tcccgtttac
     4861 cgagccttct gcagaagttg acgtcatggg taaaaacggt aaatggctgg aagtgctggg
     4921 ctgcgggatg gtgcatccga acgtgctgcg taacgttggc atcgacccgg aagtttactc
     4981 tggtttcgcc ttcgggatgg ggatggagcg tctgactatg ttgcgttacg gcgtcaccga
     5041 cctgcgttca ttcttcgaaa acgatctgcg tttcctcaaa cagtttaaat aaggcaggaa
     5101 tagattatga aattcagtga actgtggtta cgcgaatggg tgaacccggc gattgatagc
     5161 gatacgctgg cgaatcaaat cactatggcg ggcctggaag ttgacggtgt agaaccggtt
     5221 gccggtagct tccacggcgt ggtcgttggt gaagtggttg agtgtgcgca gcatccgaac
     5281 gctgacaaac tgcgtgtgac aaaagtaaat gtcggcggcg atcgcctgct ggatatcgtc
     5341 tgcggtgcgc caaactgccg tcagggcctg cgtgtggcgg tagctaccat tggtgctgtt
     5401 ctgccgggtg atttcaaaat taaagcggcg aaactgcgtg gcgaaccgtc tgaagggatg
     5461 ctgtgctcct tttctgagct gggaatttct gacgatcaca gtggcattat cgaactgcct
     5521 gcggatgcgc cgattggcac cgacatccgc gaatacctga aactcgatga taacaccatc
     5581 gaaatcagcg tgacaccaaa ccgtgccgac tgcttaggta tcattggtgt tgcgcgtgac
     5641 gttgccgtgc tgaatcagct gccgctggtt gaaccggaaa tcgttccggt tggtgcgacc
     5701 atcgacgaca cgttgccgat tacagtcgaa gcgccggaag cctgcccgcg ctatcttggc
     5761 cgtgtggtaa aaggcattaa cgttaaagcg ccaactccgc tgtggatgaa agaaaaactg
     5821 cgtcgttgcg ggatccgttc tatcgatgca gttgttgacg taaccaacta tgtactgctc
     5881 gaactgggcc agccgatgca cgctttcgat aaagatcgca ttgaaggcgg cattgtggtg
     5941 cggatggcaa aagagggcga aacgctggtg ctgcttgatg gcactgaagc gaagctgaat
     6001 gctgacactc tggtcatcgc cgaccacaac aaggcgctgg cgatgggcgg catctttggt
     6061 ggcgaacact ctggcgtgaa tgacgaaacg caaaacgtct tgctggaatg cgctttcttc
     6121 agcccgctgt ctatcaccgg tcgtgctcgt cgtcatggcc tgcatactga tgcgtctcac
     6181 cgttatgagc gtggcgttga tccggcactg cagcacaaag cgatggaacg tgcgacccgt
     6241 ctgctgatcg acatctgcgg tggtgaggct ggcccggtaa ttgatatcac caacgaagca
     6301 acgctgccga agcgtgcaac catcactcta cgtcgtagca aactggatcg cctgataggc
     6361 catcatattg ctgatgagca ggtaactgac attctgcgtc gtctcggctg cgaagtgacc
     6421 gaaggcaaag acgaatggca ggcagttgcg ccgagctggc gtttcgatat ggagattgaa
     6481 gaagatctgg tcgaagaagt cgcgcgtgtt tacggctaca acaacatccc ggatgagccg
     6541 gtacagacaa gcctgattat gggtactcac cgtgaagctg acctgtcgct caagcgcgtg
     6601 aaaacgctgc tcaacgacaa aggctatcag gaagtgatca cctacagctt cgttgatccg
     6661 aaagtgcagc agatgatcca tccaggcgtt gaagccttac tgctggcaag cccgatctct
     6721 gttgaaatgt cagcaatgcg tctttctctg tggaccggcc tgctggcaac cgtggtgtac
     6781 aaccagaacc gtcagcagaa ccgtgtgcgc attttcgaaa gcggtctgcg tttcgtacca
     6841 gatactcagg caccgttggg cattcgtcag gatctgatgt tagccggtgt gatttgcggt
     6901 aaccgttacg aagagcactg gaacctggca aaagagaccg ttgatttcta tgatttgaaa
     6961 ggcgaccttg aatccgttct cgacctgacc ggtaaactga atgaggttga gttccgtgca
     7021 gaagcgaatc cggcactgca tccggggcaa tccgcagcga tttatctgaa aggtgaacgt
     7081 attggttttg ttggagttgt tcatcctgaa ctggaacgta aactggatct taacggtcgc
     7141 actctggtgt tcgaactgga gtggaacaag ctcgcagacc gcgtggtgcc tcaggcgcgc
     7201 gagatttctc gcttcccggc gaaccgtcgt gacatcgcgg tggtggtcgc agaaaacgtt
     7261 cccgcagcgg atattttatc cgaatgtaag aaagttggcg taaatcaggt agttggcgta
     7321 aacttatttg acgtgtaccg cggtaagggt gttgcggagg ggtataagag cctcgccata
     7381 agcctgatcc tgcaagatac cagccgtaca ctcgaagaag aggagattgc cgctaccgtc
     7441 gccaaatgtg tagaggcatt aaaagagcga ttccaggcat cattgaggga ttgaacctat
     7501 ggcgcttaca aaagctgaaa tgtcagaata tctgtttgat aagcttgggc ttagcaagcg
     7561 ggatgccaaa gaactggttg aactgttttt cgaagagatc cgtcgcgctc tggaaaacgg
     7621 cgaacaggtg aaactctctg gttttggtaa cttcgatctg cgtgataaga atcaacgccc
     7681 gggacgtaac ccgaaaacgg gcgaggatat tcccattaca gcacggcgcg tggtgacctt
     7741 cagacccggg cagaagttaa aaagccgggt cgaaaacgct tcgcccaaag acgagtaatc
     7801 tgatctaact aaaaaggccg ctatgcggcc ttttttcttt tcactgtcga agagtcaccg
     7861 taaaatcaac gccatgacac ttcagcagaa cggataccat gctgacactt gcccgccaac
     7921 aacagcgaca aaatattcgc tggttattat gcctgtcagt tttgatgctg ctggcgcttc
     7981 tcttaagcct ttgcgccggt gaacaatgga tttcgccagg tgactggttt actcctcgtg
     8041 gcgaactgtt cgtctggcaa attcgcctgc cacgtacgct ggctgtattg ctggttggtg
     8101 cggcgctggc tatatccggc gctgtaatgc aggtgttgtt tgaaaatcct ctggcagaac
     8161 ctggactact tggcgtctct aacggcgcag gcgtggggct tatcgccgcg gtattgcttg
     8221 ggcaagggca actccccaac tgggcgctag ggctgtgtgc gattgctggc gcgcttatca
     8281 tcactttaat actcttacgt ttcgcccgtc gtcatctttc gaccagtcgg ttattgctgg
     8341 ctggcgttgc attagggatt atctgcagcg cactaatgac gtgggctatc tacttttcca
     8401 cctctgttga tttacgtcag ctgatgtact ggatgatggg cggttttggc ggcgtagact
     8461 ggcggcaaag ctggctgatg ctggcattga tccccgtgtt gttgtggatc tgttgtcagt
     8521 ccaggccgat gaatatgtta gcacttggcg agatctcggc gcggcaactg ggtttacccc
     8581 tgtggttctg gcgcaatgtg ctggtggcag cgaccggctg gatggttggc gtcagtgtgg
     8641 cgctggcggg tgctatcggc tttattggtc tggtgatccc acatattctt cggttgtgtg
     8701 gtttaaccga tcatcgcgta ttacttcccg gctgcgcgct ggcaggggcg agcgcattgc
     8761 tgctggccga tattgtagcg cgcctggcat tagctgccgc agagctgcct attggcgtgg
     8821 tcaccgcaac gttgggtgcg ccggtgttta tctggttatt gttaaaagca ggacgttagc
     8881 cgcaaaaaga cggtctatga ttaaaagcta agatttttac tgaccacacc caggagaaac
     8941 gatgcaagat tccattctga cgaccgtagt gaaagatatc gacggtgaag tgaccacgct
     9001 ggagaagtac gccggtaatg tgctgttaat tgtcaatgtc gcctcaaagt gtggcttaac
     9061 gccgcaatat gagcagttgg agaatattca aaaagcctgg gccgatcgag gttttgtggt
     9121 gctgggattc ccgtgcaacc agtttctgga acaagaaccg ggcagcgatg aagagattaa
     9181 aacttactgt accaccacat ggggggtgac gttcccgatg ttcagtaaga ttgaagttaa
     9241 tggcgaagga cgccatccgc tgtatcaaaa attgattgcc gcagcgccga ccgcagtcgc
     9301 gccggaagag agcggattct atgcccgtat ggtcagcaaa ggccgtgcac cgctgtaccc
     9361 ggatgatatt ttatggaatt ttgaaaaatt cctggttggc agggacggaa aagtcatcca
     9421 gcgtttttcc ccggatatga cgccggaaga tcccattgtg atggaaagca ttaaactggc
     9481 gttggcaaaa taatgtctat tgtgatgcag ttacaggatg ttgcggaatc tacccgcctg
     9541 gggccgcttt caggcgaggt tcgggctggg gaaatcctgc acctggtggg gccgaatggc
     9601 gcgggtaaga gtaccttact ggcgcgaatg gccggaatga ccagcggtaa gggaagcatt
     9661 cagttcgcgg ggcaaccact ggaagcatgg tccgcaacaa aactcgcgct gcatcgcgcc
     9721 tatctttcac aacagcagac gccgccgttt gcaatgccgg tctggcacta cctgacactg
     9781 catcagcacg ataaaacgcg taccgaacta ctgaatgatg tcgcaggggc gctggctctt
     9841 gatgacaaac tcggtcgtag caccaatcaa ctttccggcg gtgaatggca acgcgtacgt
     9901 cttgctgcgg tggtgttgca aatcacacca caagccaatc ccgcaggcca attgctgctt
     9961 cttgatgagc cgatgaacag tcttgatgtt gcgcaacaaa gtgtgttaga caaaattctg
    10021 agcgcgctgt gtcagcaagg actggcgatt gtgatgagca gtcacgatct caaccacacg
    10081 ttgcgtcatg cgcatcgggc gtggttgcta aaaggtggaa aaatgctggc cagtggacgc
    10141 agagaagagg tgctcacgcc gccaaatctg gcgcaggcct atgggatgaa ttttcgccgt
    10201 ctggatatcg aaggtcacag aatgctgatt tcgaccatct gatttcatta gtatcttgaa
    10261 aatcgggtaa ttacgacgct aaattaatgc aagaataaaa aacagaggaa ccgtacggaa
    10321 tgcgtttctg ccttattttg atcacagcac tgtttctggc cgggtgtagc caccataaag
    10381 caccgccgcc aaatgccaga ctttctgatt cgattaccgt tattgccggt ttgaacgacc
    10441 agctacaaag ctggcatggc acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg
    10501 actgttcggg atttgtggtt gtgacgatgc gcgatcgttt cgatttgcat ctgccccgtg
    10561 aaaccaaaca acaagcctct attggcacgc aaattgataa agacgagttg ctgcctggtg
    10621 acctggtctt tttcaaaacg ggttccggac aaaatggttt gcatgtaggt atttacgata
    10681 ccaacaacca atttatccac gcctctacca gcaagggagt gatgcgttcc tcacttgata
    10741 atgtctattg gcagaaaaat ttctggcagg cgagacgaat ctagtatgtc gaaaaattga
    10801 cggcgatgat acatcgccgt caatttactc ctgaatacag tagtattttg cagacaaaaa
    10861 ttccgtaaat ggattattcg tttataatca ggataatcta aaaaaaggcc ccctgaaaaa
    10921 gtggttaaca tggaacaaat gaaagttact ccttctgtaa ttgttatgtc ttatggcctc
    10981 atccctttta gccggatact gaaaaacatc cttcgagagg gacggttacc atgaagattt
    11041 ttttggagaa tctttatcat tctgattgct atttcctgcc gataagagat aaccagcaac
    11101 tccttgtcgg cgttgagtta attacccatt tctccagtga agatggcacg gttcgtatcc
    11161 cgacgagtcg ggttatagcg caacttaccg aggagcaaca ttggcaatta ttttcagagc
    11221 agttggaatt actaaaatca tgccaacact tttttattca acataaactc tttgcctggc
    11281 ttaatttaac gccacaagtg gctacattat tattagacag ggataattac gcaggcgaac
    11341 tattaaaata tccgtttatt gaactgttga ttaatgagaa ttacccgcat ctcaatgaag
    11401 gaaaagataa tcgcgatctt ttgtccttat cgcaaatgta tccccttgtg ttaggcaatt
    11461 tgggagcagg taatagcaca atgaaagcgg tttttgatgg tcttttcacc cgcgtaatgc
    11521 tggagaaagg ttttattcag caacaaatca cgcatcgctc cttcgaaccg tttattcgtg
    11581 cgatccaggc gcaaatttcc ccttgttgca attgcatcat cgcggggggc attgatacgc
    11641 cagaaatatt ggcacagatt acccccttcg atttccatgc tctccaggga tgcctgtggc
    11701 ctgccgttcc tataaatcag ataacgacgt tggttcagcg ataaccctcc tgtttgctgg
    11761 tgtttaagac gagagtaacc gtctacacta tcaaacagat aggatctatg accctgtctt
    11821 ttattacccg ctggcgcgat gaattgccag aaacctatac agcactttcc cctacgcctt
    11881 taaataatgc ccggctgatt tggcataata ccgaactcgc taacacgctg agtattccat
    11941 cgtcgctgtt taaaaatgcc gcaggtgtct ggggcggcga aaccctactg cctggtatgt
    12001 caccactggc acaggtttac agtggtcatc agttcggcgt ctgggcgggc caactgggtg
    12061 atgggcgcgg cattttactt ggcgaacaac tgcttgctga tggcactaca atggactggc
    12121 atctgaaagg tgctggcctg acgccttatt cgcgaatggg tgatggacgg gcggttttac
    12181 gttcgacgat acgagaaagt ctcgccagtg aggcgatgca ttatctgggc attccgacga
    12241 cccgcgcgtt aagtatcgtc accagcgatt cgccagtgta tcgggaaacg gtggagccag
    12301 gcgcgatgct gatgcgtgtg gcaccaagtc atctgcgctt tggtcatttc gaacattttt
    12361 actatcgccg cgagccggaa aaggttcgtc agttggctga ctttgccatt cgtcattact
    12421 ggtcacatct tgaagacgat gaggacaaat accgtctctg gtttaacgat gttgtcgcac
    12481 gtaccgcgtc gttaattgcc caatggcaga cggtcggctt tgcacatggg gtgatgaata
    12541 ccgacaacat gtcgctgctg ggactgacgc ttgattacgg gccgtttggt tttcttgatg
    12601 attacgaacc cggttttatt tgtaatcact cggatcatca agggcgttat agctttgata
    12661 atcaacctgc tgtcgcgttg tggaatttac agcgtctggc gcagacattg tcaccatttg
    12721 ttgccgtaga tgccctgaat gaggccctgg acagctatca gcaggttttg ttgacgcatt
    12781 atggacaacg gatgcggcag aaactgggct tcatgacgga gcaaaaagag gataacgcgc
    12841 tactgaatga attattcagt ctgatggcgc gagagcgcag cgattatacc cgcacattcc
    12901 gcatgctgag tctgaccgag cagcacagcg cggcgtcacc gctacgtgat gagtttattg
    12961 atcgtgcggc atttgatgac tggtttgccc gttatcgggg gcgtttgcaa caagacgagg
    13021 ttagcgatag tgagcgtcag caactgatgc aaagcgttaa cccagctctg gtattgcgca
    13081 actggttggc gcaacgggcg attgaagcgg cagaaaaggg tgatatgatg gaattgcacc
    13141 gcctgcatga ggcgttgcga aatcctttca gcgacagaga tgatgactat gtcagtcgtc
    13201 cacctgactg gggtaaacgg ctggaagtca gctgctcgag ttaaaggcta cttagttaac
    13261 agcagcttgc cagcctgagt tttacggagc agatattctt gcccgtcatg atcgataatc
    13321 agtttgccat ccggacctaa cagtgtctgg ctggtaattc gccggatggg ctgcggggaa
    13381 gcggtcttgt gattggcatc cgtttcaggc gtgagttttc tgctatccgt ataacgcata
    13441 aaaattaata ataattgata taacaatgat tcttattatc aatattatgg caggccagag
    13501 caagcagtaa tgtgttgaga agacaacaag ttgcgcagaa atgcccatcc catcaggaat
    13561 gggcacgcat tcagaagcgg gtatctaccg cagaggcgag tttttcgacc aggcgttcgg
    13621 tatcttccca gcccagacac gggtcggtaa tggattgacc gtaagtgagc ggctgaccgc
    13681 cgacgatttt ttgcgttcct tcgcgcagga aactttccgc cataattcca gcaatctccg
    13741 tagagccatt gcggatttgc tgacaaatat cctcacaaac ttctaactgg cgacggtgct
    13801 gcttctggca gttaccgtgg ctgaaatcca ccaccagatg ttcaggtaaa tcaaactcgt
    13861 gcagcgtatc gcaggctgcg gcgatatcat cggcatgata attcggtttt ttgccgccac
    13921 gcataataat gtggccatac gggttgccgc tggtctgata gatggtcatc tgaccatttt
    13981 tgtctggcga gaggaacata tggctggcgc gggctgcgcg gatagcatcc acggcaatcc
    14041 gcgtgttgcc atcggtacca tttttaaaac ctaccggaca ggagagtgct gaagccattt
    14101 cgcggtggat ctgactttcg gtagtacgag cgccaatcgc gccccaactg attaaatcag
    14161 caataaactg accggtcacc atatcgagga actcagtcgc ggtagggaca cccagctcat
    14221 ttacctgtaa aagtaatttg cgcgccagct ccagaccgtg atttacccga tagctgccgt
    14281 ttaaatccgg atcggagatt agccctttcc agccgacaac agttcgtggt ttttcaaaat
    14341 aggtgcgcat tacgatttcc agccgtgact ggtactggtt gcgcagcgac tgcagacggg
    14401 tggcgtactc cattgcagcg gtgagatcgt ggatcgagca ggggccaata atgaccaaca
    14461 gtcgcttatc ttcaccattc agtatttttt caattctgcg gcgggagtcg gtgacatggg
    14521 tggcgacgcc aggcgttacg ggataccgta gcgcgagttc ggcgggcgtt accaggctct
    14581 caatacgcgc agtacggagt tcgtcagttc tgttcattac aagtctcaga aatttgttgc
    14641 ttctacggca ggactaccgg aaagtgatgc gcatcacgat aaaccaatcg cagctgattt
    14701 caagtgcgag gtgtgtcaca cctcgccggg aggttagcat gataacaaaa aaacaaacca
    14761 atgcactagt tctctagtac attcggcgac taaggcccat gatatcgagg atcttggtgg
    14821 caatctcttc taccgaataa ttggtactgt taatccacgg gatctgattt ttacggtaca
    14881 aggcttccac ttccgcgact tccatcctgc actgacgaag cgaggcatag cgactgttct
    14941 cccgacgttc ctcgcgaatc gccgccagac gttccgggtc gatagtcagg ccgaacaatt
    15001 tatgctgaag cggtttgagc gacgcgggta gcaccagatt atccatatcg tcggcaataa
    15061 aggggtagtt tgccgcgcgg ataccaaatt gcattgccag atacagactg gtgggggttt
    15121 taccacagcg agaaacaccg agcaggatca cctgagcctg atccagattg cgcaacgaaa
    15181 tgccgtcatc gtgggcgagg gtgtaatcaa tcgccgcaat gcgcgcatca tatttattga
    15241 gattattagg gttaaggcca tgggtacgat gagcaatcgg cgttggatcc agtttcatct
    15301 cttgttgtag cggggcaacc agcgcctgaa cgatatcctg gcaaaagcct tcactttgca
    15361 agatgatggc gcgaatctcc ggcaacacga tggagtagaa gaccagcggg cgcacgcctg
    15421 tctggtgata aattgcgtca atctgatcct tcactgcccg tgcacggctc tcattttcga
    15481 caaacggcag cgtgatgctg ctgatagtga cgggaaattg tgacattact gcgtgtccta
    15541 atacctccgc agttattgcc gtaccatcag aaatataaaa aacgtggcga tcaacagcat
    15601 tatccatttt gttcttcccg tgatgcagac ataattctat gaaagcataa attaaaaacg
    15661 cacagaagcg taggacgtaa tgtctggttt ataaaatgaa ccttcaattt tattttttat
    15721 gaaaacagca tttcattttt atggtttcgt ttataccgat ggtttatgtg gaaattgtcg
    15781 aatagagcag gtttgcgcaa cgctgggatc agtcttaaaa agtaaaaaaa tatatttgct
    15841 tgaacgattc accgtttttt tcatccggtt aaatatgcaa agataaatgc gcagaaatgt
    15901 gtttctcaaa ccgttcattt atcacaaaag gattgttcga tgtccaacaa tggctcgtca
    15961 ccgctggtgc tttggtataa ccaactcggc atgaatgatg tagacagggt tgggggcaaa
    16021 aatgcctccc tgggtgaaat gattactaat ctttccggaa tgggtgtttc cgttccgaat
    16081 ggtttcgcca caaccgccga cgcgtttaac cagtttctgg accaaagcgg cgtaaaccag
    16141 cgcatttatg aactgctgga taaaacggat attgacgatg ttactcagct tgcgaaagcg
    16201 ggcgcgcaaa tccgccagtg gattatcgac actcccttcc agcctgagct ggaaaacgcc
    16261 atacgcgaag cctatgcaca gctttccgcc gatgacgaaa acgcctcttt tgcggtacgc
    16321 tcctccgcca ccgcagaaga catgccggac gcttcctttg ccggtcagca ggaaaccttc
    16381 ctcaacgttc agggttttga cgccgttctc gtggcagtga aacatgtatt tgcttctctg
    16441 tttaacgatc gcgccatctc ttatcgtgtg caccagggtt acgatcaccg tggcgtggcg
    16501 ctctccgccg gtgttcagcg gatggtgcgc tccgacctcg catcttctgg cgtgatgttc
    16561 tccattgata ccgaatccgg ctttgaccag gtggtgttta tcacttccgc atggggcctt
    16621 ggggaaatgg tcgtgcaggg tgcggttaac ccggatgagt tttacgtgca taaaccgaca
    16681 ctggcggcga atcgcccggc tatcgtgcgc cgcaccatgg ggtcgaaaaa aatccgcatg
    16741 gtttacgcgc cgacccagga gcacggcaag caggttaaaa tcgaagacgt accgcaggaa
    16801 cagcgtgaca tcttctcgct gaccaacgaa gaagtgcagg aactggcaaa acaggccgta
    16861 caaattgaga aacactacgg tcgcccaatg gatattgagt gggcgaaaga tggccacacc
    16921 ggcaaactgt tcattgtgca ggcgcgtccg gaaaccgtgc gctcacgcgg tcaggtcatg
    16981 gagcgttata cgctgcattc acagggtaag attatcgccg aaggccgtgc tatcggtcat
    17041 cgcatcggcg cgggtccggt gaaagtcatc catgacatca gcgaaatgaa ccgcatcgaa
    17101 cctggcgacg tgctggttac tgacatgacc gacccggact gggaaccgat catgaagaaa
    17161 gcatctgcca tcgtcaccaa ccgtggcggt cgtacctgtc acgcggcgat catcgctcgt
    17221 gaactgggca ttccggcggt agtgggctgt ggagatgcaa cagaacggat gaaagacggt
    17281 gagaacgtca ctgtttcttg tgccgaaggt gataccggtt acgtctatgc ggagttgctg
    17341 gaatttagcg tgaaaagctc cagcgtagaa acgatgccgg atctgccgtt gaaagtgatg
    17401 atgaacgtcg gtaacccgga ccgtgctttc gacttcgcct gcctgccgag cgaaggcgtg
    17461 ggccttgcgc gtctggaatt tatcatcaac cgtatgattg gcgtccaccc acgcgcactg
    17521 cttgagtttg acgatcagga accgcagttg caaaacgaaa tccgcgagat gatgaaaggt
    17581 tttgattctc cgcgtgaatt ttacgttggt cgtctgactg aagggatcgc gacgctgggt
    17641 gccgcgtttt atccgaagcg cgtcattgtc cgtctctctg attttaaatc gaacgaatat
    17701 gccaacctgg tcggtggtga gcgttacgag ccagatgaag agaacccgat gctcggcttc
    17761 cgtggcgcgg gccgctatgt ttccgacagc ttccgcgact gcttcgcact ggagtgtgaa
    17821 gcagtgaaac gtgtgcgcaa cgacatgggg ctgaccaacg ttgagatcat gatcccgttc
    17881 gtgcgtaccg tagatcaggc gaaagcggtg gttgaagaac tggcgcgtca ggggctgaaa
    17941 cgtggcgaga acgggctgaa aatcatcatg atgtgtgaaa tcccgtccaa cgccttgctg
    18001 gccgagcagt tcctcgaata tttcgacggc ttctcaattg gctcaaacga tatgacgcag
    18061 ctggcgctcg gtctggaccg tgactccggc gtggtgtctg aattgttcga tgagcgcaac
    18121 gatgcggtga aagcactgct gtcgatggct atccgtgccg cgaagaaaca gggcaaatat
    18181 gtcgggattt gcggtcaggg tccgtccgac cacgaagact ttgccgcatg gttgatggaa
    18241 gaggggatcg atagcctgtc tctgaacccg gacaccgtgg tgcaaacctg gttaagcctg
    18301 gctgaactga agaaataaaa taaatccccg gcggcgttca gtcgccgggg ttatgtgatc
    18361 cccgaagatg aaacttattc aatctcttca cagacatcct gcgttaatcg ccgtataata
    18421 tcttttctta acaaaaactt ttgtatttta cctgaggcag tgcgcggtag tttttcgatt
    18481 accacgatat gttcaggata tttatatttt gcgacccgtt tacggctaaa aaaagccacc
    18541 atctcttcca gcgataatga atgatgcggc gctttcagca cgacataagc gcatgatcgt
    18601 tcacctaaac gttcatcggg cattgcaacc acacaggcat cgtgaatttt aggatgctgc
    18661 aataaaatat cttccacttc acggctgcta atattttcgc cgccgcggac aataatatct
    18721 tttttgcgtc cggtaatttt tatatagcca gcctcatcca tacggcagag atcgccgctg
    18781 taataccagc cttcttcatc cagcgcacgg gcggttaatt caggttcatc aaaatacccc
    18841 ataaacacat tggggccacg cgatgcttct tcaccttcgc aacctggcgg taaggtcttg
    18901 cgtgcgtcat cgaccacttt aatctctaca cctgcggcag cgtaaccatc ggtatgcata
    18961 aagcgcgaca aagaatcatc gagattcacc accgcatgcg gcgaactttc tgtggaacca
    19021 taaacactta ataatttaat gctgcgctgc tggcattcgc gcgccacttt tttggggatt
    19081 gtggtaccgc cgcaaagaaa gaagcgcagc gctgaaaggt ctgcgggttg tttctctagt
    19141 aaattcaaaa gatcatagac aaacggcgtt gcgcctagca tacaggtgca acgctgctgc
    19201 tcaagcagcg cgagacacgc atcaggagtg aaaatatcta acaacacgct gcgagcgcca
    19261 attaagaatg gtgccgttac gccatgcaga aagcccgttg cgtgaccaag tggcgcaggc
    19321 atcataaaga catccagcca ggtcagattc agccgcgcgc aataagcccg ctcactggcg
    19381 agaatattgt tatgcgttag catcacgccc tttggcagac cctcggttcc ggaggtaaac
    19441 agcaccgcag ctaattcatc gccgtgggtc gttatcgccg tggtcagtgg gatattgtcg
    19501 gcgataatct gacttaatga gagggaagag gtggcgggag ccagtttgtc cacgccgaca
    19561 atttgttgta gttgtggaag ctgattttgc agcggcagga ttaaatctac cggacgcgtt
    19621 tgtttaaaca acgtcggtgc aaagaacatt tttgcctgac acttattgag cacctacacc
    19681 agttctgctt cccgccagga aggcaacagc ggcacggaaa ccgcaccgat tttcaggcag
    19741 gcaagataga taacggtaaa ttcacaccag ccaggcagtt gaaatgcgat gcgatcgcct
    19801 gattcaatgc ccttcgctaa catccagttt gccagacagc tcgcggcgtg atcgagcgcg
    19861 ctataggtgt acgatgcacc atgattatcg accacggcaa ttttgtctgg catcgcacga
    19921 gcggtctgct gccagtaatc ggccagtgaa gcatcgcccc atagcccttg ctgacgatac
    19981 gccgcacgac gttgttcgtt aaacgttaat gtcactttca ttttggactc tcgaaacaga
    20041 acatcaggcc cgagttgcgg gcctgtggga tgcatgatta gccgtagcga aaatcaatac
    20101 cgaaggtgcc tgcgggatat tgccactgtt cgagaatagt gttaccgcac agcacccgac
    20161 aggtgccgca ctccagacaa ccggcgggat caaaatgaat gtttcctacg tcatcctgct
    20221 tataaagtcc ggcagggcag gctttcatta atttacggaa ttcattgata tcgggatttt
    20281 ccgtcagaat gatatgcggg tggccctcat caacatggaa tttattgacg cctaatttga
    20341 tgtcgacgtt aaccgtagcg ttctggctca tagcgcggtt gctcccttaa tgccatcttt
    20401 cagcaagttg atcagcccaa ttttcttcgc gtgtcccatg atcattttgc gtaccggctg
    20461 gtttggtttg ccgtcaatgg tgaacatctc gttcatgatg tcggctacca ttcgtgggta
    20521 ttggctaaac aggcgcgggt tttccatcag cgccgggatc ttgcgaaaat gctgcatatc
    20581 acgcatgacg cagctttgtt ccagctcacg tttgtattgc gccagactgt tggcggagaa
    20641 atccgcgcgt tctttggcgg cgatcaccgt tgtggcggca gcctgagccg atgcaatggc
    20701 taaatccatg ccgcggaccg taaaacccaa attcaggcag aagcctgcgg cgtcaccaac
    20761 gatcatcacg ccctcgttaa ccagttgcgg caccattgcc agaccgcctt ccggcaccat
    20821 atgcgcggaa tattcaagca gtttgccgcc gctaatcagc gggcgaatgg cggggtgttg
    20881 tttaaaatct tccagcattt gcggcacgct tttttgcgca tgggcgatat cacccaatcc
    20941 acaaaccagc cccaaggata tggaatcctt gttggtatag agaaatcccc cgcccatcag
    21001 gccgtcagaa ggggaaccgg caaacagcca ggcggcacct tcctcgcccg taatattaaa
    21061 gcgatcgttg atctgttctg gtgtgaggcc aataacctct ttaacaccaa cagcgtaatg
    21121 atgcggatcg gaagcgggaa ccattcccag cgagcggcca agcatcgagt taacgccatc
    21181 agccagaatc accacattcg cttcgagaat atcatcccca gcctgcacgc cagtgacctt
    21241 gtttccttca cgaaccagcg cgtcgacgcg aacgcctggg ataaactgcg cgcccgcctg
    21301 ctcggcttgt tccatcaacc acgggtccag acgattacgc aatacggtat aagatgcgtg
    21361 ttgcggaaca tctggttgct cgcggtgaaa atcgagggta acggcgcttt cttcggttaa
    21421 gaaggagatt ttctcgcgtg tgaccttgcg ttctaccggc gctgatactg caaagcctgg
    21481 aatgattgct tcaagtgtgt gggcataaag acgcccgccg gtcatgtttt tacatccggc
    21541 actgtcgccg cgttctatca ccaggacatc cagcccggct cgtgccatga ccagtgcggc
    21601 aacgctacca gcaacgcccg caccgaccac aatggcatca aatttgtcat ccgacatgcg
    21661 ttatgctccc ttcccgctgc gtttacagcg ggagatgtgg ttgaaagggg aattagcggc
    21721 tcaactggct aatcagggca gggacgactt tgtagatatc gcccaccaga ccgtagtcgg
    21781 catagttgaa gattggcgca tttttatctt tattgatggc gacaatcact tttacgccgt
    21841 tgccgccaac catatgttgg atctgcccgg agatccccag cgtcaggtag agatcggatt
    21901 tcagcaacac gccggagaca ccgatataac gttcacgctc catccagttc tcgccttcgg
    21961 caattggacg tgaacagccg acttcagcat tcagcaccgc cgccagttcg tggaccattt
    22021 tcaggtcatc ctgcgccgcc agaccacgac cgacgccaac cacacgtttt gctttgctca
    22081 ggtccacgct gcttgcggct ttcgcacggc gttcgcgaca gagaatttca tgacgcggag
    22141 caacataagg taccgtttct gtcgggcact gatgagaggt atcactggtg cacggttcct
    22201 gaacaccggg tgcaagggta atgatcgcca gcgggctgtt gatcttttcc tgagcgaacg
    22261 ctaacccgcc atacatccgg tgttcggcgc aaatgtgacc atcgacaata tccaccgccg
    22321 tggcatcgtt caccagcgcc gcattcagtt gcacacttaa ccgtgctgcc agagctttac
    22381 cacgtttcgt cgcggccaac agcaacatag cggggtgttt atctttcagc agggcagcaa
    22441 tgctttcggc gtaattttca gtgcgttgca gcgcgtcgtt ttgctcaaga acataaatac
    22501 attttggatc ataaggcata actgcctgcg cctggtcggt attttgtaca atggcataca
    22561 cctgttggcc ccattgctga gcgccgccaa acagttcagc ataacgttca ggattatcgc
    22621 taaagaccca gacgctgttt aattgactca tcgtttcttc tccgtaggct tcagttcagg
    22681 gctttcttca gatgttccgc cagctcggca atggcctccg gcgaatcgtt atcgagaatg
    22741 atgtgcttac gttctgtttg cggcggtacg cgaatgccga ccagttcagc aagtggcgcg
    22801 ctctggctcc agtcaatatc acttgcctgc cactgattta ccggtttttt acccgcgccg
    22861 agaatggctt tcatcgaagg aatgcgtggc acgttaatat cggaggtgac gcagagcacg
    22921 gctggaacag agagttcaat aacttcaaca tcatcttcaa gcgtgcgttc aatcaccagt
    22981 gtattgccct gacgctgaat agcactcact gcattaatca ccggaagttg cagaatttct
    23041 ccgaccagca agccaacctg ctgggcataa aggtcgccgg aaccttcacc aaagatcagt
    23101 aaatcgaagc cgatcttttc aattgctgcc gccagcgctt ttgcggtatc gagaggcagt
    23161 gcatgttcaa gttgcgcatc ctgcaccaaa tacaggctgt gcggcccgcg ggatagcacg
    23221 tctttgcgca ctttcgagtt ctgcaacaat gagccgccaa cggtcagcgc ggctatctca
    23281 tcgtcatctg ttgcgagctg gcttgcagct tcaatggcat tgagatcgaa ctggctgatt
    23341 ttggcgtcgg cattgtcgaa attcagggtg tattctggag tgacaacaat gtcctgttct
    23401 tcaggcacca gcttaaagca ggttattatt ttcatggttt ctcctgtgag tcgcatacgg
    23461 aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc ggtgcggggt
    23521 tttcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg cgatggctaa
    23581 aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct tgcattcagt
    23641 gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata cacgccgtat
    23701 tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc atgtatcaac
    23761 gctgttttga taatgccagt gaaacgctgt ttgtcgccgg taaaacgcca cggctttcac
    23821 gttttgcatt tagcgatgat ccaaaatggg agtctggaca tcacgttcat gacaatgaaa
    23881 ccgagctgat ttacgtcaag aaaggggttg caaggtttac catcgattct tcgttatatg
    23941 tcgcacatgc ggatgacatt gtggtgatag aacgcggcag gctgcatgcg gtggcctctg
    24001 acgttaacga tccggccacg acgtgtacct gtgcgctgta cggctttcag tttcaggggg
    24061 ctgaggaaaa tcagttactg caaccgcatt cttgtccggt aattgccgcg gggcagggaa
    24121 aagaagtcat aaaaacctta tttaatgagc taagtgtgat tttgccgcaa agtaaaaata
    24181 gccaaacatc ttcgttatgg gatgcatttg cctatacatt agcaattctt tactacgaaa
    24241 actttaaaaa tgcttatcgt tcggagcagg ggtatattaa aaaagatgtt ctgataaaag
    24301 atattctttt ctatctgaat aataattatc gcgaaaaaat cactttagaa cagttatcga
    24361 aaaaatttcg tgccagcgtc agttatattt gccatgaatt taccaaagag tatcgtattt
    24421 cccctattaa ctatgttatt caacggcgta tgacggaagc aaaatggtca ctcactaata
    24481 ctgaattatc acaggcagag atctcctggc gtgtgggtta tgaaaatgtc gatcactttg
    24541 ccaaactgtt tttgcgccat gtcggttgtt cgcccagcga ttaccgtagg caatttaaaa
    24601 attgttttgc ggaacaagaa atcttatctg aatttcctca accggtaagt cttgccggat
    24661 aaatctggcg gcgcactttg ttaaaaagct gcgccgcctg cagattattt gttctgatag
    24721 tctttcagga tctgccgacc tgctacgtaa atcataattt cgtctgtacc gccgccgata
    24781 cgttcacaac ggacatcacg ccagaagcgg gagacgcgcg cctcatcggt atagcccaga
    24841 ccgcccatga tttgaatcgc atcatcaatg acttccattg cggtacgtgc acagtacagt
    24901 tttgccagcg ccgcgctggt gcgcagtgat tgatgctgat cggcttgcca tgccactttc
    24961 agcaccatgt tgcgcatgtt gtcgatctta atcgccatca gcgccagttt ttcctggatc
    25021 atctggttat gcccaatggg tttaccaaaa gcgatacgct ggttggcata gcgagcggca
    25081 tcttcaaagg cacactcggc aaagccggtg ctgcgcgcgg cgttgatcag gcgctccatc
    25141 tcaaagttgt acatcacatt gaggaaaccc attccttctt cgcccaccat gtcgctctct
    25201 tcaacttcca cgttgtcgag atagacttcg caggtgctga gcatatgcca gccgatttta
    25261 tgcagcgggt taatcttaat gccgggctta ctggagtcga cccaccacag ggtgaaggct
    25321 tttttgggat ctttcggttg cggatcgcgc gccaacacca gcatatacgg gtactctttc
    25381 gcaccggtaa taaaggtttt ctgtccgttg atgtaaacct tgccgttttt acgcgtgtaa
    25441 gtggtagtgg cactgttgtt gtctgagcct gcgcctggct ccgtcaacgc cagggcatag
    25501 gcgggatcac ccgtttccag tgtgctttct gccgttttac gtagctgctc tgcagaaccg
    25561 aaacggcgca tactgtgaat acattgaccg ttggtaatca aaaatgccgg agcaccgcat
    25621 tttgacactt ccatcagcgc cagcatctgg gtaacgtaat ccgcagggat accgccgaac
    25681 tcttccggca caccaagcat ggaaataccg ttatccgcca gtgcccgcat aaactcacgc
    25741 gggtatgtcc cgttttgatc gcaggtgcgg gaatactctt ccggaaagtt agtcgtaatc
    25801 aactcgcgaa tactggccag cagcagttct tgttcttcag ttaaagaaaa atctatcgtg
    25861 atactcctgt taatgagccg cttcaggcag gacaaaaccc atcgccgcat cgataaataa
    25921 tctttcgtcc atcagtttga gttctggcga aatcactggg gtgaaatcca ttttgtcgag
    25981 aatatctttt tgtaaatcga cgccaggagc gatttcgatt aaatgcaggc cgtcttcttt
    26041 cagcgtgaat acagcacgtt cggtgatgta acgaacatcc agtccccgtt cgagcgcgat
    26101 ttttccgcgg aaagtaattt ccggtagttc ccgaataaat ttcttcaccc gtccttcctg
    26161 gacgatattt aatttgccgt cggtaatttc tgtttttaaa ctgctcgcag ttaacgtgcc
    26221 gcagaaaata attttcttcg aggtggcact gatatcaata aatccaccgg tacccatgat
    26281 tttgccattg aatttatgca cgccgacgtt accgtgctgg tcgacttcag caaaactcaa
    26341 ataacaaaca tccagaccac caccgtgata aaaatcaaac tgggacgtca tatccagaat
    26401 ggcgcgggta ttcacgttcg cgccaaaggc gataccctgt gaagtaattc cgccaatcgg
    26461 acctgtttct accgtcagaa taaagtcatc agcacaacct tcttcccgag cgaccaggcc
    26521 aatgccgtca gcaataccga cgccgacatt ccccaccgcg cctttacgca tttcgaataa
    26581 tgcgcgccgc gcaactaatt tacgttggtt taggggcagg ctaagtttgg tactgtcatc
    26641 aagggtgaag tcaccagaaa taaagcggtt aaccggtgca ccgccataca gttgggtttg
    26701 atccggatcg accaccacaa tatccaccag ataacccgga atacggacag atttaggatg
    26761 cagcgtggct ttcttaacca ttttctgcac ctgcatcatc acaataccgc cgttattgtg
    26821 cactgcctgg gcaataacca atgcgtcgag atacatcacc tcatcttcaa aaatggcgta
    26881 gccttcgctg tcgcaggtgg tagcgcgaat gaaggcgata tctggcgcaa tcgctttgta
    26941 atagagatat tctttgttat caaactcgac cagtttaatc aggtcttctt tagtgacttc
    27001 attcagtttg ccgccttgct ggcgtggatc gacaaatgtc ccgatgccaa tatcactaat
    27061 aataccaggc tggtgggctg cggcggcgcg taaggtttgt gtaagtacac cttgtggata
    27121 gttataagca ataattttat tttgttctgc gagatcagaa atacgcggcg attgtcccca
    27181 gtgaccgcat aatgcccatt tcaccagacc ttcttgcgcc agcggactaa taccgcggtc
    27241 ggcgcgatcg ccaagccctg ttggactaat aatcgataaa ttacgtggcg tttgagtctg
    27301 tttatattta tcagcaagag caataattaa cgtggtggct tccagaatac cgccgccagc
    27361 gcctaacaca caaagtgttg cttcgtcggg aatataattc accgcttcct gtgccgacag
    27421 gaccggcact cgtccattaa tacgaggtgg ttttacaggt ttcattgtta attcctgatt
    27481 caggtgtgat tttccctacg ccagatggag aattagcgca cagagactca cgaaatattt
    27541 ttataaaata cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa
    27601 atattattgc ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga
    27661 tttgccctgg cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt
    27721 caccagccag acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat
    27781 cggcatactg ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac
    27841 tttgcggcat cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac
    27901 gggcaatgat ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt
    27961 tcacatcatg cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata
    28021 actccagatc gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat
    28081 aagcctcggt ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca
    28141 gcggtttttc tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat
    28201 tggagaggtc ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat
    28261 aggcgagagc ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt
    28321 taggtgcgcc cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt
    28381 tctgcacgcg gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag
    28441 ggaaatcttt gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt
    28501 atccatcaat cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg
    28561 ggttatacac gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca
    28621 atgattcatt ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg
    28681 aagccagggc ttcagcaaag gcttgctgat cggcgagatc ggtaaccgtg acgacacaat
    28741 cggtgttttc gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac
    28801 ggttaaagag tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg
    28861 aggcacctcc ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt
    28921 taatggcgcg aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat
    28981 taacgatggt gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt
    29041 cacacgccag ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta
    29101 atccttcaat tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg
    29161 gcaatcccgc tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag
    29221 gataagccat caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta
    29281 acctctatgc ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc
    29341 attctcagga ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag
    29401 ggccagcagc gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag
    29461 ataaccggta atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact
    29521 ggtgactttg gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa
    29581 aatgccgcca gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga
    29641 agggtacagg tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc
    29701 ccagatgggc cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga
    29761 gcccatactg taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc
    29821 catcgcatat ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc
    29881 gaacagtacc gatgaaacac cttccagcca gactaacggt ttgctgttca tttgcggtaa
    29941 ttcattagct acgctggcgt ccaccaactg gctggggaat ttgcttttca acagcatcag
    30001 cgtgatcagt acaaacagaa tacccggaat aatcagccca tagccgtacc agatattatt
    30061 gagcaacata tagctcacca gcattgggta gaacatttgc ccaaatgaca ccatcgcttt
    30121 aaccagtatg accgccgaac cagaggcttt cggaaagcat tccatgagcg cggggtagcc
    30181 acccgtatcc agcgctgagt tagcgatacc tacgcacact gccagaccgt aggcgagagt
    30241 taaattcggg caagcgggaa taccaaagaa gaatagcaga tacattatta ctgccattaa
    30301 tatcaccgcc cgacgaccaa acttatcgga gatcacaccg aagaataaaa tactgatcaa
    30361 tcgccccaaa ccgataccgg aaattaaata ggcaatgccc gcgttgtcag tggaaaactt
    30421 ttctgccaga gatgtcatat tttgggcaag cgtaataaca ctaatgccgt gcaggaaata
    30481 gctgaagtaa atacaaagaa cagccaggat aaatggcgtg ctgaaagcct tattttgaga
    30541 cattttcaca gctccttgct gaaagataat ttcgttaccg tacgtattgt tctccgacgg
    30601 tttacataat tactatactg aagatgattt tccctgggta catggcataa agtttatgtt
    30661 tccaggaata ttgcatgtca tagtttgttt ttgatcgcga ttttccttag ttagcgcaga
    30721 aagagttatt tatccgtaag atcttgcatg acctgaaaag aatattgcat tacccacccg
    30781 gagcgacatt ttcctttagc gagtgatgcc gcgttttttt gcggctgcgt aagccgataa
    30841 acagtgccag taaaaaaccg atggcagcga tggcggtatc gaaccacata atatcggcaa
    30901 tacttctttg ggacagatga gccgtaatca acggaatagt aaaggtcgca atactgcccg
    30961 cactgtaata gatccctgta gctttacctt tagcgtaagg gaaacgttca gccattaacg
    31021 tcaggccaat ttgcacaaca cctccagcag aggtaaaacc aatgacaaaa gcaaatatta
    31081 tcaccacata aaatgtggga tgcaggcaga cggtaaacag agcaataaat gagataaagg
    31141 tgtacagcat cagtaatgtt gttgggcgaa cggtattacg aatgagtgga gcggtaataa
    31201 atacacaaag cagcgaaccc acggtgtaga tactgagtag tttgatcgac atagtgtatg
    31261 acatgcctgc aacaaattgt ccgtactgtg ccagccactg gctaaccaga taaaacgttg
    31321 ccattgagat atagccatat aaggtataac tggctaaatc gataattgaa cagcgatgtt
    31381 ccgtagagct ggtggttttc tttatgacag gtaagcgacg acccggatgg ggtgggaacg
    31441 tacaacggta taaaaacaga gcgttaataa acataatgcc tgcagcaatc ataaaggacc
    31501 aaccgaacca cagttcagcc cacactaaca ggctaatgat tagcggtaat aaaaattgtc
    31561 cgctggaaac aaatgcttta attaaaatat tggctgtccc aggtgagcgt ggaaaagctt
    31621 ccatcaaact gggataagtg cctgcatcga gaaaactgtt tgccattccc gccagaaagc
    31681 caaaaacata agcgataatg atgttattgg tctgcaggat gccaaaaaag aaggccatat
    31741 agcagcacat cccgagcatg ataaaagggc ggcgaccaaa gcgatcggat aataatcctg
    31801 caaaaagcaa gacacttaat cgaccaatgc ccagcgatga gataactatc gagacacccg
    31861 cggcattagt ctgccaaagt gtctccagcg aggccatatt caggctcatc aaaatgacgc
    31921 ccataccatg caccaggtaa ttaaaataca acccaagtgc ggtagggtaa tagggatttt
    31981 tcataggttc tacttagcaa aggtgcttta aaaagtgtag cgatgttgtg tcagtatcca
    32041 ttcattattg ttgtaacaac gacaattcct ttctgtggaa aaattaacaa agccgctcca
    32101 gatcgtgtgc atataattca gcggctaccg attgatagat cttccagtca ataaaattgc
    32161 catcagggta gacttgttga aacgcggtta agtcggggaa aaaacgcatg gtgttgttgc
    32221 cgatgcggtt atttattttc tctatgatgg catgaagatg tttttttcgt tgctgacgca
    32281 tagccaacag ttgctcgact acacaatcag gaatggcgca tttgccattt tcccactgac
    32341 gccagctttc actgtcaccg gtctgggcaa tccaggtcgc gcattcatca atagtcatgg
    32401 caaaaatatg gcggagtgcc tggagttcat aagcgttcat cgtgtaatgg ctgcatgaaa
    32461 taaacaataa aatataaata acattatcag cctgctgacg gcttgaagag agtcacagta
    32521 tcttgtgcaa cattattgga aagagtgagg ggcgaggtag cgaagcggaa aaatgttaaa
    32581 aaaaagccca tcgtgggaga tgggcaaaga ctacacacag caattcgttg tttcactcag
    32641 gggatttcca tgcttataaa tcaatatgtt gatttataac cgtgagctaa tagtaggcat
    32701 acggactttt tgcgtcgatc agattcgtct cattagttta aaaactgtaa agaatttgcg
    32761 acaggtacta ccagctgaat ggcggtaagc aggaaaatat ggggaagagc aagcaaatat
    32821 caattagcgg tgattaacaa tcaggcggca gagccgcctg atgagaaatt acttattcgg
    32881 tttctcgatt tcgccaagtt cttcgagaat ttcttccggt tggtccgtcg gcggtgggac
    32941 ttcttccacc cacgcggcaa acagacgcca ggaaacggct aacagaaccg gaccaataaa
    33001 cagaccgatc atcccgaaag caatcaaacc accaataacg ccagagagga tcaggatcag
    33061 cggtaaatcg gcacccatgc gaattaacat tgggcggatg acgttatcca gcgtgccaac
    33121 cacaccgctc cacactaaca ataccgttcc ccaggtggta tcgccggtcc agtagagcca
    33181 gataatcgcc ggaatcagta ccggcaacgg gccaagctgg acaaggcagg agaggatcat
    33241 taacaccgtt agcaacgttg cataaggtac gccggatacg gcgaggccga taccgccaag
    33301 cactgcctgt actaacgccg tcaccaccac acccagcgcc accgcgcgga tagcctgtgc
    33361 cgccagcagc acggcggcat ctccgcgaac gcctgccaga cgggttgcaa aatggcgaat
    33421 gccttgtgcc acctgttcac cgcgccagta cagcagggca ctgaagagaa gcatcagcgc
    33481 acaatgcacc ataaagcgcc cgatatgcgc cgcctgccca acgaaccagg tggtggtggt
    33541 gccaatataa gggcggactt tcgccatgat cgccgtaccc cccatatcca gcaagttgtg
    33601 ccagcctgca tacagcttcg cgccaatcac cggaatggta ttaagccacg ccagatcggg
    33661 taacgtcatg tcgccgctgg aaatggcttt aattagcggg ccgctgccgt cgacgatact
    33721 gttaaccagc aaagcaatag ggatgataaa caccatcact aataacagcg tcatcaccag
    33781 aacggcgaga gaacggcggc caaacatgat cttttgcaaa cgtaacaata ccggccaggt
    33841 ggcgataacc accgtaccgg cccatgcaaa gccgagaata aagggttgaa caatccacag
    33901 acatgccaca atcatgatgg ctaaaaacag caccgaaagc agaatttgtg cgacatccct
    33961 gggctgacga acatttacca taactacttt tcaccttttt tgtgcgtcaa atcgtcgacg
    34021 cgacgtgaac acgcgaaact cacctgcata atgatgagca atttcagcgg tttttaacag
    34081 gccgattctg cccttaattc tgatgggcgt gttagaaaaa aatgtggtac aacaactcag
    34141 gcaacacgca aacgatttac tcgtcgtatt tcaagttgca tgtgctgcgg ctacgttcgt
    34201 tcaccccagt cacttactat tgtaagctcc tggagttcac tcgcttgccg ccttcctgca
    34261 actcgaaatc catagagtaa ataaacatca cctttcagtg cgctattgca gccgcctgaa
    34321 agaatagtgt atcgacctac atcacagacc gcaggaaagg gtcaatataa tgattccaca
    34381 gatttcccag gcacccggcg tcgttcaact ggtgcttaat tttttgcaag agctggagca
    34441 acaaggtttt accggcgatg cggcgacaag ttatgccgat cgtctgacaa tgtcgaccga
    34501 caacagtatt taccaacttc tccccgatgc ggtggtattt ccgcgttcaa ccgcagatgt
    34561 ggcgctgatc gcccgtcttg ccgcgcagga acgctattca tcgctgatct ttaccccccg
    34621 cggcggcggc accggcacta acggtcaggc gctcaaccag gggattattg ttgatatgtc
    34681 ccgccatatg aaccgcatca tcgaaattaa ccctgaagag ggctgggtgc gcgttgaggc
    34741 cggggtgata aaagaccaac tcaatcagta cctgaaaccg ttcggctact tttttgcgcc
    34801 ggaactttcg accagcaacc gggcaacgct cggtgggatg atcaataccg atgcatccgg
    34861 tcagggatcg ctggtctatg gcaaaacgtc agatcacgta cttggcgtac gcgcggtgtt
    34921 gttggggggc gatattctcg atacgcaacc tttacccgtc gagctggcgg aaacgctggg
    34981 taaatccaat accacaatcg ggcgaattta taacacggtt tatcaacgtt gccgtcagca
    35041 acgccagtta atcatcgaca acttccccaa acttaaccgc tttcttaccg gttacgatct
    35101 gcgtcatgtc tttaacgatg agatgaccga gttcgacctg acgcgcattc tgacgggttc
    35161 agaagggacg ctggccttta ttaccgaagc gcggctggat attacgcgct tgcctaaagt
    35221 gcgccgtctg gtgaatgtca aatatgactc ttttgactct gcgctgcgta acgcgccgtt
    35281 tatggttgag gcgcgggcgc tttcggtaga gacggtggac tcaaaagtgc tgaatctggc
    35341 gcgggaagat attgtctggc attccgtcag cgagttgatt accgatgtgc ctgacaaaga
    35401 gatgctcgga ctgaacattg tggaatttgc tggtgatgat gaggcgctga ttgacgagcg
    35461 agttaatgca ctctgtgtgc ggcttgatga gctgattgcc agccaacagg caggtgtgat
    35521 tggctggcag gtgtgccgcg agctggcggg ggttgaacgt atctatgcga tgcgcaaaaa
    35581 agccgttggt ctgcttggca atgccaaagg tgccgctaag ccaattccgt ttgctgagga
    35641 tacctgcgta ccgccggaac atctggccga ttatattgct gaatttcgcg cgctgctcga
    35701 cagccacggc ttaagctacg gtatgttcgg tcacgtcgac gcaggtgtct tgcacgtccg
    35761 tccggcactg gatatgtgcg atccgcaaca agagattttg atgaagcaaa tctctgatga
    35821 cgtggtggcg ctgactgcga aatacggtgg tttgttgtgg ggcgagcacg gcaaaggttt
    35881 tcgcgctgaa tacagcccgg cgtttttcgg tgaggaactt tttgcagaac tgcgcaaagt
    35941 gaaagcggca tttgacccgc ataaccgact caacccaggg aagatttgcc cgccagaagg
    36001 tctcgatgcg ccgatgatga aagtggacgc ggtgaagcgc ggtacattcg atcggcagat
    36061 ccccattgcg gtacgccagc agtggcgcgg tgcgatggag tgtaacggca acggtttatg
    36121 cttcaacttt gatgcccgta gtccgatgtg tccgtcgatg aagatcaccc agaaccggat
    36181 tcattcaccg aaagggcgcg caacgctggt gcgtgaatgg ctgcgcttgt tggcggatcg
    36241 cggcgttgac ccactcaaac tggaacaaga actgcctgaa tccggcgtca gtttgcggac
    36301 gttaattgcc cgcacgcgca atagctggca tgcgaataaa ggcgaatatg acttctcaca
    36361 cgaagtcaaa gaggcgatgt cgggctgtct ggcctgtaaa gcttgttcga cccagtgccc
    36421 catcaaaatt gatgtgccgg agtttcgctc tcgttttctg cagctctatc acacccgtta
    36481 tttacgcccg cttcgcgacc acctcgttgc tacggtcgag agctacgcac cgctgatggc
    36541 acgcgcgccg aaaaccttta acttcttcat taaccagccg ctggtgcgca aactctcgga
    36601 aaaacatatc ggcatggttg atttgccact gctgtcggtc ccctcgctac aacaacaaat
    36661 ggtggggcat cgctcggcaa acatgacgct ggaacagctt gaagctctca atgcagagca
    36721 gaaagcgcgc acggtgttgg tggtgcagga cccctttacc agctattacg atgcgcaagt
    36781 ggtggcggat tttgtccgtc tggttgaaaa attaggtttc cagcctgtgt tactgccatt
    36841 ttcgccaaat ggcaaagccc agcatatcaa aggctttctt aatcgttttg cgaagacggc
    36901 gaaaaagacg gcggatttcc tcaaccgtat ggctaagctt gggatgccaa tggtgggcgt
    36961 cgatccggcg ctggtacttt gttatcgcga tgaatataaa ctggcgctgg gcgaggaacg
    37021 tggcgagttt aacgtcttac ttgcgaatga atggctggca agcgcacttg agtcacatcc
    37081 agtggctaca gtcagcggtg aatcatggta tttctttggt cactgtaccg aggttaccgc
    37141 tttgccgggc gcgccagcac aatgggccgc gatatttgcc cgttttggcg cgaaactgga
    37201 aaatgtcagc gtgggttgct gcggcatggc agggacttac ggacatgaag cgaaaaacca
    37261 tgaaaattcg ctcgggatct atgagttatc ctggcatcag gctatgcagc gactgccgcg
    37321 taaccgctgt ctggcgaccg gatattcctg ccgtagccag gtaaaacggg ttgaaggcac
    37381 gggggtacgc catcctgtgc aggctttact ggagattatt aaatgatatg gaaacggaaa
    37441 atcaccctgg aagcactgaa tgctatgggt gaaggaaaca tggtggggtt cctggatatt
    37501 cgctttgaac atattggtga tgacaccctt gaagcgacaa tgccagtaga ctcgcggaca
    37561 aagcagcctt tcgggttgct gcatggagga gcatccgtgg tactggccga aagtatcggt
    37621 tccgttgccg gttatttatg taccgaaggt gagcaaaaag tggttggtct ggaaatcaat
    37681 gctaatcacg tccgctcggc acgagaaggg cgggtgcgcg gcatatgtaa accgttgcat
    37741 ctcggttcgc gtcaccaggt ctggcagatt gaaatcttcg atgagaaagg gcgtttgtgc
    37801 tgttcgtcac gattgacgac cgccattttg tgacgttgtc accagaaaag tgtgacgcat
    37861 attgcttttg gtcattatct gcacagtaaa cggtggtata ctgtgcaggt cttgtctaaa
    37921 agcgaggatg ctatgtctac tcaacttgat cccacccagc tggccattga atttttacgc
    37981 cgtgaccaga gcaatctttc acctgcccaa tatctcaaac gtctgaaaca actagaactt
    38041 gaatttgctg acctgcttac tctctcttct gctgaattaa aagaagaaat ctactttgcc
    38101 tggcgtctgg gcgtgcattg atcctgactt aatgccgcaa gttcgccaaa tcgacctgga
    38161 ttaacctccg tccaggttcg atgaccttct taatactgct tatctaccgc ttgatattgt
    38221 tcaaaaataa tactaatcgc agtttgtgtt aaaacggcgg gttagcttta tgagattatt
    38281 cttatcgccc cttcaagagc taagccactg agagtgccgg agataagcgc cggatggggt
    38341 agaaaccctt aagcctgtgt cgcacagact taagggtttt cttatttctg aacgctgcac
    38401 ttttttacct atctcttaaa tagtcatttt caatgcaact ttttcttttt catcacagcc
    38461 ttaatgctcc ttagataata accattatct aacaatgaga tacctaattc ttagcgtgcc
    38521 tgttaaccca cacatcaggg gctatgctta ttaaaagtag gcaaaaagca tgttttacgg
    38581 taaagcccct gcgtttgctg ggttgaactg ataatcatta tcactaacat gctgttatac
    38641 gctgaaagcg atgaagtgag gtaaatagat ggacatgcat tcaggaacct ttaacccaca
    38701 ggatttcgcc tggcaaggct taacgctgac acccgcagcg gcgatacaca tccgcgagct
    38761 ggtggcaaag cagccaggta tggtcggcgt gcgcttaggt gtgaagcaaa cgggctgcgc
    38821 gggctttggc tatgtgctcg acagtgttag cgagccggac aaagacgatc tgctgtttga
    38881 acacgacggc gcgaagctgt ttgtcccgct gcaatcgatg ccgtttattg atggcacgga
    38941 agtcgatttc gttcgtgaag gacttaatca gatattcaaa tttcacaacc ctaaagccca
    39001 gaatgaatgt ggctgtggcg aaagctttgg ggtataggcg gtactatgtc tcgtaatact
    39061 gaagcaactg acgatgtcaa aacctggacc ggcggcccgc tgaattataa agaaggattc
    39121 ttcacccagt tagccaccga tgagctggca aaggggataa acgaagaggt ggtgcgcgca
    39181 atttcggcga agcgtaatga gccggagtgg atgctggagt ttcgtctaaa cgcctatcgc
    39241 gcatggctgg agatggaaga accgcactgg ttgaaagcgc actacgacaa gctgaattat
    39301 caggattaca gctactactc agcaccatcg tgcggtaatt gtgacgacac ttgcgcgtct
    39361 gaacctggcg cggtgcagca aactggcgcg aacgcctttt taagtaaaga ggtggaggcg
    39421 gcgtttgagc agttgggcgt tcccgtgcgg gaaggcaaag aggtggcggt ggatgccatt
    39481 ttcgactctg tttcggttgc caccacttat cgcgaaaaac tggcggagca gggaattatt
    39541 ttctgttcct ttggtgaggc gatccacgat cacccggaac tggtgcgtaa atatctcggc
    39601 accgtggtgc cggggaatga caacttcttt gcagcactta atgcggcggt agcctctgac
    39661 ggtacgttta tttatgtgcc taaaagcgtg cgctgcccga tggaactttc cacctatttt
    39721 cgcattaacg cggaaaaaac cgggcagttt gagcgcacaa ttctggtggc cgacgaagac
    39781 agctacgtca gctacattga aggctgttcc gctccggtgc gtgacagcta tcagttgcac
    39841 gcggcagtgg tggaagtcat catccataaa aacgccgagg tgaaatattc cacggtacaa
    39901 aactggttcc ctggcgataa caacaccggc ggtattctca acttcgtcac caagcgtgct
    39961 ttgtgcgaag gcgaaaacag caaaatgtca tggacgcaat cagaaaccgg gtcagcgatt
    40021 acgtggaaat atcccagctg cattttgcgc ggcgataact ccattggtga gttttactca
    40081 gtggcgctga ccagcggtca tcagcaagcg gataccggca ccaagatgat ccacatcggt
    40141 aaaaacacca aatcgaccat tatctcgaaa gggatctctg ccggacatag tcagaacagt
    40201 tatcgcggct tagtgaaaat catgccgacg gcaaccaatg cgcgcaattt cactcagtgc
    40261 gactcaatgc tgattggcgc taattgtggg gcgcatacct tcccgtatgt cgaatgtcgc
    40321 aataacagcg cacaactgga gcacgaggca acgacatcac gtattggtga agatcaactg
    40381 ttttactgcc tgcaacgcgg gatcagcgaa gaagacgcca tctcgatgat tgttaacggt
    40441 ttctgcaaag acgtgttctc agaactgccg ctggaatttg ccgttgaagc acaaaaactc
    40501 ctcgccatca gtcttgaaca cagcgtcgga taaggaataa acatgttaag tattaaagat
    40561 ttacacgtca gcgtggaaga taaagctatc ctgcgtggat taagcctcga cgttcgtccc
    40621 ggcgaagttc acgccattat ggggccaaac ggttcgggca aaagtacctt atcggcaacg
    40681 cttgccgggc gcgaagatta tgaagtgacg ggcggcacgg ttgagttcaa aggcaaagat
    40741 ttgcttgcac tgtcgccgga agatcgtgca ggcgaaggca tctttatggc cttccagtat
    40801 ccggtggaga ttccaggcgt cagtaaccag tttttcctgc aaacggcact taatgcagtg
    40861 cgcagctatc gcgggcagga aacgctcgac cgctttgatt ttcaggattt gatggaagag
    40921 aaaatcgctc tcctgaagat gccggaagat ttattaaccc gttcggtaaa cgttggtttt
    40981 tccggcggcg agaaaaagcg caacgatatt ttgcaaatgg cggtgctgga accggagtta
    41041 tgcattcttg atgagtcgga ctccgggctg gatattgacg cattaaaagt ggtcgccgat
    41101 ggcgtgaact cgctgcgtga tggcaagcgc tcattcatca ttgttacgca ctaccaacgc
    41161 attctcgact acatcaagcc tgattacgtt catgtgctat atcagggacg aattgtgaaa
    41221 tccggcgatt tcacgttggt caaacaactg gaggagcagg gttatggctg gcttaccgaa
    41281 cagcagtaat gcgctgcaac agtggcatca cttgtttgaa gctgaaggga caaaacgctc
    41341 accgcaagca cagcagcatt tacaacaatt gctgcgtacc ggactgccga cacgtaaaca
    41401 tgaaaactgg aaatatacgc cgctggaagg gctgaccaat agtcagtttg tcagcattgc
    41461 gggagatata tccccacagc agcgtgatgc cttagcgtta acgttagacg ccgtgcggct
    41521 ggtgtttgtc gatggacgtt acgtgcccgc gctgagcgat gcaattgaag gcagcggata
    41581 tgaagtgagc attaacgacg accgtcaggg gttacccgac gctattcagg cggaagtgtt
    41641 tctgcatttg acggaaagcc tggcacaaag cgtgacgcat atcgccgtga agcgcggtca
    41701 acggccggca aagccattgc tgttaatgca tatcacccag ggcgtggcag gtgaagaggt
    41761 gaacactgcc cattaccggc atcatctgga tctggcggaa ggtgccgaag caacggtgat
    41821 cgaacatttt gtcagcctta atgatgctcg tcattttacc ggggcacggt tcactatcaa
    41881 tgtcgcagcg aacgcccact tgcagcatat caagctggcg tttgaaaacc cggtcagtca
    41941 ccactttgcc cataacgatt tgttgctggc tgacgatgcc accgcattta gccatagttt
    42001 cctgctgggt ggcgcagtgt tacgacacaa caccagtacg caactcaatg gcgaaaacag
    42061 cacgctgcgg atcaatagcc tggcgatgcc ggtgaaaaac gaggtgtgtg atacccgtac
    42121 ctggctggaa cacaataaag gtttttgtaa cagccgacag ttgcacaaaa ctatcgtcag
    42181 cgacaaaggc cgcgcggtat ttaacggttt gatcaacgtc gcgcagcacg ccatcaaaac
    42241 ggatggtcag atgaccaaca ataatctgct gatgggtaaa ctggcggaag tggatacaaa
    42301 accgcagctg gaaatctatg cagatgatgt gaaatgcagc cacggcgcga cggtggggcg
    42361 tattgatgat gaacagatgt tctatctgcg ctcgcgcggg atcaatcagc aggatgccca
    42421 gcagatgatc atttacgcct ttgctgccga actgacggaa tcactgcgtg atgaggggct
    42481 taaacagcag gtgctggccc gaatcggtca acggctgcca ggaggtgcaa gatgactttt
    42541 tccgtcgaca aagtgcgggc cgactttccg gtgctttcgc gtgaggtaaa cggtttgccg
    42601 ctggcttatc tcgacagcgc cgccagtgca cagaaaccga gccaggtgat tgacgccgag
    42661 gccgagtttt atcgtcatgg ctacgcggcg gtgcatcgcg gtattcatac cttaagcgcc
    42721 caggcgaccg agaaaatgga gaacgtgcgc aagcgggcat cgctgtttat taatgcccgt
    42781 tcggcggaag agctggtgtt cgtccgcggc acgacggaag ggatcaatct ggtcgccaat
    42841 agctggggca acagcaatgt gcgggcgggc gataacatca tcatcagtca gatggagcac
    42901 cacgctaaca ttgtcccctg gcagatgctt tgcgcacgcg ttggcgcaga gctgcgtgtg
    42961 atccctctca accccgacgg tacgctgcaa ctggagacgc tgcctacgct gtttgatgag
    43021 aaaactcgcc tgctggcaat tactcatgtc tccaacgtgc ttggcacaga aaatccactg
    43081 gcggaaatga tcacgcttgc gcaccagcat ggcgcaaaag tgctggtgga tggcgctcag
    43141 gcggtgatgc atcatccggt ggatgttcag gcgctggatt gcgactttta cgtgttctcc
    43201 gggcataaac tgtatggccc caccggaatt ggcattcttt atgtgaaaga agccttgttg
    43261 caggagatgc cgccgtggga agggggcggt tctatgatcg ccaccgtcag cctgagtgaa
    43321 ggcactacct ggaccaaagc accatggcgg tttgaagccg gtacacccaa taccgggggc
    43381 atcattggtc ttggcgcggc gctggagtat gtttcggcgc tggggcttaa tagcatagcc
    43441 gagtatgaac agaatctgat gcattatgcg ctatcacagc tggaatctgt accggatctc
    43501 actctctatg gcccacaaaa caggcttggc gttattgctt ttaatctcgg taaacaccac
    43561 gcctatgatg ttggcagttt tctcgataat tacggcattg ctgtgcgtac cggacatcac
    43621 tgcgctatgc cattaatggc ctattacaac gtccctgcga tgtgtcgggc gtcgctggcc
    43681 atgtataaca cccatgaaga agtggatcgt ctggtgaccg gcctgcaacg tattcaccgt
    43741 ctgctgggat aacagggagg cactatggct ttattgccgg ataaagaaaa attgctgcgt
    43801 aattttttac gctgcgccaa ctgggaagag aaatatctct acattattga gctgggccag
    43861 cgtctgccag aattacgcgc cgaagaccga agcccacaaa atagcattca gggctgccag
    43921 agtcaggtgt ggattgtcat gcgccagaat gcgcagggaa ttattgaatt gcagggcgac
    43981 agcgatgcgg cgattgtgaa agggcttatt gcggtcgtct ttattctcta cgatcagatg
    44041 acgccgcagg atattgtcaa tttcgatgtg cgtccgtggt ttgaaaaaat ggcgctcacc
    44101 caacatctca ccccatctcg ttcacaaggt ctggaagcga tgattcgcgc aattcgcgcc
    44161 aaagccgctg cacttagcta aactaaaggg acagctttca tccggttggc tcacattgcc
    44221 ggatggtcaa tgtttacact gacctgcaca ggagtgtttt ggtttcaggt gaacataagc
    44281 gccatccgac gtttcagcgt gagtctccgg caaatacagg aggtttacaa tgaaacgcgc
    44341 gtctttgctt acactcacgc ttatcggcgc ttttagcgcc atccaggctg cctgggcggt
    44401 tgattatccg ctaccaccaa ccggaagccg actggttggg caaaatcaaa cgtatacggt
    44461 gcaagaaggg gataaaaacc ttcaggctat tgcccgacgt tttgatactg cggcaatgtt
    44521 gatccttgaa gccaataaca ctatcgcccc ggtgccaaaa cctggtacga cgataactat
    44581 tccttcacaa ctgttattac ctgatgcacc gcgtcagggg attatcgtta accttgcaga
    44641 gctgcgcctt tattattatc cgccgggaga aaatattgtg caggtttatc caataggtat
    44701 tggattgcag gggctggaaa cgccggtgat ggaaacgcgt gttgggcaga aaatccctaa
    44761 cccaacctgg acgcctacgg caggcattcg tcagcgttcg ctggagcgtg gcattaaatt
    44821 accgccagtc gttcctgccg gaccaaataa cccgctagga cgttacgcac tgcgcctcgc
    44881 gcatggtaat ggcgaatacc tcattcatgg caccagtgcg ccggacagcg ttggtttgcg
    44941 cgtcagttca gggtgtattc gcatgaatgc accggatatt aaagccttgt tctccagcgt
    45001 gcggacggga acgccggtga aagtgatcaa cgaaccggtg aaatattccg tggaacctaa
    45061 cgggatgcgt tatgttgaag tacatcgacc actatcagca gaagaacagc aaaacgttca
    45121 gacaatgcca tacacactgc cagcaggctt tacgcaattt aaagacaata aggctgtaga
    45181 tcagaagtta gtcgataaag cgttgtatcg tcgggcaggg tatccggttt cggtgagcag
    45241 tggagcaact cccgcagcca gcaatgcgcc ttcagtagag tcagcgcaga atggtgaacc
    45301 agagcaaggg aatatgttac gcgcgacgca gtagtggtaa atagcagaca aaaaaaatgg
    45361 cgcacaatgt gcgccatttt tcacttcaca ggtactatta cttgcggtat ttagtagcca
    45421 tgttgtccag acgctggtta gcacgagctg cgtcatcttt agcagcctga acgtcggaac
    45481 gcactgcgtt cacgtcgttg ctcagctggt caactttagc gttcagagtc tgaacgtcag
    45541 aagacagctg atcgatttta gcgttgctgg agcaacctgc cagcagagta gaacccagga
    45601 ttaccgcgcc cagtaccagt ttagtagctt tcattattaa taccctctag attgagttaa
    45661 tctccatgta gcgttacaag tattacacaa agttttttat gttgagaata tttttttgat
    45721 gggaaggcac ttatttttga acgttcgctc aaagaagcat cgaacgctgg gttttagata
    45781 aaaaaattga gagaaaacag gatgcttcca tcggattcat cttagataaa gtgagattat
    45841 atagtgaaat ccgatttgat tttttattgc ttctggttat cgattaaata aaaaaagcgc
    45901 ccatcagggc gcttcgatat acaaattaat tcacaaaagc aatattacag gacgtgaaca
    45961 gatgcggtgt tagtagtgcc gctcggtacc agtgcaccag aaaccataac tacgacgtca
    46021 cctttgtgtg ccagaccgct ctgcagagcc agttctttac ccagacggta gaaatcatca
    46081 gtagaagtga tctctttaac cagctgcggc acaacgcctt tgctcagtac caactgatga
    46141 gccgtttttt cgttggtggt cagtgccagg atggtggcat ccgggaagta tttacgtact
    46201 gcgcgagcag atttaccgcc ctgggtagca accacgatca gcggagcatc cagtttttca
    46261 gcagtttcaa cggcaccacg gcataccgct tcggtgatgc gcagtttacg gttgtcattg
    46321 ttgaactcga gacggctgtt catcacgcgg tcggtacgtt cgcagatggt cgccatgata
    46381 gaaaccgctt ccagcgggta tttacctttt gcggattcac cagacagcat cactgcgtca
    46441 gtaccgtcga ggatggcgtt tgcaacgtca ccggcttctg cgcgagtcgg gcgtgggttt
    46501 ttgatcatgg aatccagcat ctgggtcgca gtgataacga ctttacgtgc acggatacat
    46561 ttttcgatca tcatcttctg ggcgaagata acttcttcta ccgggatttc tacacccagg
    46621 tcgccacgcg caaccatgat gccgtcagag gcttcgagga tttcgtcgaa gttgttgagg
    46681 ccttcctggt tttcgatttt ggagatgatg tggatgtttt cgccgccgtg cgctttcagg
    46741 tgctcacgga tttcgataac gtcagaacgc ttacgaataa aggaagcagc aacaaagtct
    46801 acgccttttt cgcaaccaaa gatcaggtcc tgtttgtctt tttcagccag tgctggcaga
    46861 gcaatggaaa cgccaggcag gttcacacct ttgttttcgc ccaggtcacc gttgttcagc
    46921 actttacaga taactttgtt accttcaatg gcggtaactt ccataccgat cagaccatcg
    46981 tcaaccagta cggtgttgcc aacagacagg tcagtagtga aaccttcata cgttaccgca
    47041 accatttcgc tgttgccgat aacagattta tcagtggtga aagtaaaggt ctgaccagct
    47101 ttcagagaaa catcgttacc gccttccagt ttcatggtgc ggatttccgg acctttggta
    47161 tcaagcagga tagcggcggt tttaccagtt ttgctcatca cgttgcgcag attctgaatg
    47221 cgctgaccgt gttctgcata gtcaccatga gagaagttca gacgcataac gttcatgcca
    47281 gcgtccagca ttttagctaa catctcttca gattcggttt tcggtccgat ggtgcaaaca
    47341 attttggtct ttttcatgac agtcttagtc tttaagttga gaaggatggg agaaacttgc
    47401 tttctgggcg cactgccgga aagcgaatcc ggtattacga aagtggtggt gcctcgctct
    47461 aaggataggt gacatcgaaa aagcgtgcag aggaatgtgt gcttgtgtct cagcccaacg
    47521 tgagtgcctg tttttgagtc gttgagttct acagtccaaa gtgctgaaac cattcaagag
    47581 tcaattggcg cgcattatac gctgaaatta atccaaaagg aaaacaaaaa cagcgtttca
    47641 aaaaatatac aacttgttag acaataaatt gttatcaaag atttaacgtt ccagggaaaa
    47701 ggttacgcaa aaacttcgct gtttgcgcaa cgtctggtgc cgaagttgtc agtgtgagga
    47761 taagtgaaag ttcatccggt gtataaatat atcatcgctc aattgctaca gttaaaataa
    47821 tttattgcgt tatttgtctg attttgtttg atttttgttt aattggttag attgatatat
    47881 gctgatagtt ggcatgataa ataggtataa agaatgggaa atcggacaaa agaggatgaa
    47941 ctgtaccgag aaatgtgcag agttgtcggt aaagttgtgc tggaaatgcg cgatctgggg
    48001 caggaaccaa agcatattgt tatcgcaggc gtattacgta cagcattagc caataaacgc
    48061 attcagcgca gtgagctgga aaagcaggcg atggaaaccg tcattaacgc actggtaaaa
    48121 tgaccattc
//
LOCUS       DBKEKI010000015        47371 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.015, whole genome shotgun sequence.
ACCESSION   DBKEKI010000015 DBKEKI010000000
VERSION     DBKEKI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.015"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1334
                     /locus_tag="ACUQPX_000811"
     CDS             <1..1334
                     /locus_tag="ACUQPX_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HHV9055594.1"
                     /translation="LSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKY
                     FHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAINGIAIGSNARANH
                     ANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDA
                     VNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTD
                     GVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSSTNQRRITNVAASV
                     NATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDA
                     VNYAQLKQSAQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASM
                     ASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            1702..3357
                     /gene="lldP"
                     /locus_tag="ACUQPX_000812"
     CDS             1702..3357
                     /gene="lldP"
                     /locus_tag="ACUQPX_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HHV9055595.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            3357..4133
                     /gene="lldR"
                     /locus_tag="ACUQPX_000813"
     CDS             3357..4133
                     /gene="lldR"
                     /locus_tag="ACUQPX_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HHV9055596.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            4130..5320
                     /gene="lldD"
                     /locus_tag="ACUQPX_000814"
     CDS             4130..5320
                     /gene="lldD"
                     /locus_tag="ACUQPX_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HHV9055597.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            5516..5989
                     /gene="trmL"
                     /locus_tag="ACUQPX_000815"
     CDS             5516..5989
                     /gene="trmL"
                     /locus_tag="ACUQPX_000815"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HHV9055598.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(6042..6863)
                     /gene="cysE"
                     /locus_tag="ACUQPX_000816"
     CDS             complement(6042..6863)
                     /gene="cysE"
                     /locus_tag="ACUQPX_000816"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HHV9055599.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(6943..7962)
                     /gene="gpsA"
                     /locus_tag="ACUQPX_000817"
     CDS             complement(6943..7962)
                     /gene="gpsA"
                     /locus_tag="ACUQPX_000817"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HHV9055600.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(7962..8429)
                     /gene="secB"
                     /locus_tag="ACUQPX_000818"
     CDS             complement(7962..8429)
                     /gene="secB"
                     /locus_tag="ACUQPX_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HHV9055601.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(8492..8743)
                     /gene="grxC"
                     /locus_tag="ACUQPX_000819"
     CDS             complement(8492..8743)
                     /gene="grxC"
                     /locus_tag="ACUQPX_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HHV9055602.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(8885..9316)
                     /locus_tag="ACUQPX_000820"
     CDS             complement(8885..9316)
                     /locus_tag="ACUQPX_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HHV9055603.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            9561..11105
                     /gene="gpmM"
                     /locus_tag="ACUQPX_000821"
                     /gene_synonym="pgmI"
     CDS             9561..11105
                     /gene="gpmM"
                     /locus_tag="ACUQPX_000821"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HHV9055604.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            11139..12398
                     /gene="envC"
                     /locus_tag="ACUQPX_000822"
     CDS             11139..12398
                     /gene="envC"
                     /locus_tag="ACUQPX_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HHV9055605.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNDRKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            12402..13361
                     /locus_tag="ACUQPX_000823"
     CDS             12402..13361
                     /locus_tag="ACUQPX_000823"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HHV9055606.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(13348..14382)
                     /gene="waaH"
                     /locus_tag="ACUQPX_000824"
                     /pseudo
     CDS             complement(13348..14382)
                     /gene="waaH"
                     /locus_tag="ACUQPX_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(14621..15646)
                     /gene="tdh"
                     /locus_tag="ACUQPX_000825"
     CDS             complement(14621..15646)
                     /gene="tdh"
                     /locus_tag="ACUQPX_000825"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HHV9055607.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(15656..16852)
                     /gene="kbl"
                     /locus_tag="ACUQPX_000826"
     CDS             complement(15656..16852)
                     /gene="kbl"
                     /locus_tag="ACUQPX_000826"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HHV9055608.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(17127..17984)
                     /gene="yibB"
                     /locus_tag="ACUQPX_000827"
     CDS             complement(17127..17984)
                     /gene="yibB"
                     /locus_tag="ACUQPX_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HHV9055609.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            18287..19219
                     /gene="rfaD"
                     /locus_tag="ACUQPX_000828"
     CDS             18287..19219
                     /gene="rfaD"
                     /locus_tag="ACUQPX_000828"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HHV9055610.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            19229..20275
                     /gene="rfaF"
                     /locus_tag="ACUQPX_000829"
     CDS             19229..20275
                     /gene="rfaF"
                     /locus_tag="ACUQPX_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HHV9055611.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKETWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            20279..21271
                     /gene="rfaC"
                     /locus_tag="ACUQPX_000830"
     CDS             20279..21271
                     /gene="rfaC"
                     /locus_tag="ACUQPX_000830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HHV9055612.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGVAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPRLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            21268..22476
                     /gene="rfaL"
                     /locus_tag="ACUQPX_000831"
     CDS             21268..22476
                     /gene="rfaL"
                     /locus_tag="ACUQPX_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HHV9055613.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(22512..23654)
                     /locus_tag="ACUQPX_000832"
     CDS             complement(22512..23654)
                     /locus_tag="ACUQPX_000832"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HHV9055614.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKESMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(23663..24676)
                     /locus_tag="ACUQPX_000833"
     CDS             complement(23663..24676)
                     /locus_tag="ACUQPX_000833"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HHV9055615.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKELPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDEAIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(24701..25408)
                     /gene="rfaY"
                     /locus_tag="ACUQPX_000834"
     CDS             complement(24701..25408)
                     /gene="rfaY"
                     /locus_tag="ACUQPX_000834"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HHV9055616.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAAKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(25434..26441)
                     /gene="waaO"
                     /locus_tag="ACUQPX_000835"
                     /gene_synonym="rfaI"
     CDS             complement(25434..26441)
                     /gene="waaO"
                     /locus_tag="ACUQPX_000835"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HHV9055617.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(26484..27281)
                     /gene="rfaP"
                     /locus_tag="ACUQPX_000836"
     CDS             complement(26484..27281)
                     /gene="rfaP"
                     /locus_tag="ACUQPX_000836"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HHV9055618.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(27274..28398)
                     /locus_tag="ACUQPX_000837"
     CDS             complement(27274..28398)
                     /locus_tag="ACUQPX_000837"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HHV9055619.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQSKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(28395..29453)
                     /gene="rfaQ"
                     /locus_tag="ACUQPX_000838"
     CDS             complement(28395..29453)
                     /gene="rfaQ"
                     /locus_tag="ACUQPX_000838"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HHV9055620.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYLDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            29866..31143
                     /gene="waaA"
                     /locus_tag="ACUQPX_000839"
     CDS             29866..31143
                     /gene="waaA"
                     /locus_tag="ACUQPX_000839"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HHV9055621.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            31151..31630
                     /gene="coaD"
                     /locus_tag="ACUQPX_000840"
     CDS             31151..31630
                     /gene="coaD"
                     /locus_tag="ACUQPX_000840"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HHV9055622.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(31669..32478)
                     /gene="mutM"
                     /locus_tag="ACUQPX_000841"
     CDS             complement(31669..32478)
                     /gene="mutM"
                     /locus_tag="ACUQPX_000841"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HHV9055623.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(32576..32743)
                     /gene="rpmG"
                     /locus_tag="ACUQPX_000842"
     CDS             complement(32576..32743)
                     /gene="rpmG"
                     /locus_tag="ACUQPX_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HHV9055624.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(32764..33000)
                     /gene="rpmB"
                     /locus_tag="ACUQPX_000843"
     CDS             complement(32764..33000)
                     /gene="rpmB"
                     /locus_tag="ACUQPX_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HHV9055625.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(33217..33885)
                     /gene="radC"
                     /locus_tag="ACUQPX_000844"
     CDS             complement(33217..33885)
                     /gene="radC"
                     /locus_tag="ACUQPX_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HHV9055626.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            34057..35277
                     /gene="coaBC"
                     /locus_tag="ACUQPX_000845"
     CDS             34057..35277
                     /gene="coaBC"
                     /locus_tag="ACUQPX_000845"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HHV9055627.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            35255..35713
                     /gene="dut"
                     /locus_tag="ACUQPX_000846"
     CDS             35255..35713
                     /gene="dut"
                     /locus_tag="ACUQPX_000846"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HHV9055628.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            35820..36416
                     /gene="slmA"
                     /locus_tag="ACUQPX_000847"
     CDS             35820..36416
                     /gene="slmA"
                     /locus_tag="ACUQPX_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HHV9055629.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(36453..37094)
                     /gene="pyrE"
                     /locus_tag="ACUQPX_000848"
     CDS             complement(36453..37094)
                     /gene="pyrE"
                     /locus_tag="ACUQPX_000848"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HHV9055630.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(37160..37876)
                     /gene="rph"
                     /locus_tag="ACUQPX_000849"
     CDS             complement(37160..37876)
                     /gene="rph"
                     /locus_tag="ACUQPX_000849"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HHV9055631.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            38003..38866
                     /locus_tag="ACUQPX_000850"
     CDS             38003..38866
                     /locus_tag="ACUQPX_000850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HHV9055632.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEIMQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNVE"
     gene            39054..40319
                     /locus_tag="ACUQPX_000851"
     CDS             39054..40319
                     /locus_tag="ACUQPX_000851"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9055633.1"
                     /translation="MAKARLTDIQIRAWIKAGEHFEGRADGNGLYLCFPEKYSAPFWR
                     YRYRMAGKQRTMHIGSYSDLSLSKARATVKELAARVALGHDVAGEKQARKAAALARIE
                     EEKNALRVSELAAEYYERQILPRWKHPDILRRRINKDINPQIGSLKVEDVKPRHIDAM
                     LQRIIDRGAPTVANDVLRWTKRIFDYAIKRHALETNPAAAFELSDAGGAEKSRDRWLT
                     RDELIRFFQAMRTAKGFSRENELTMKLILCLCCRKMELCAAQWDEFDLDSAVWRLPAE
                     RAKNGDAIDIPLPPPAVAWLRELHSISGGSRWVLPARKMQQRMIPHIQESTLPVALAK
                     VRGEMPGVPGFTVHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFNER
                     RHALELWADLLVALEQGSEYNVTPFKIAK"
     gene            40815..41021
                     /locus_tag="ACUQPX_000852"
     CDS             40815..41021
                     /locus_tag="ACUQPX_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9055634.1"
                     /translation="MDTLPKSGYMRPTQVMRHFQISESTLWNWVKKGKFPRPDKLSDG
                     VSRFDVSAIRKWLADRKGTTSTRN"
     gene            41043..41879
                     /locus_tag="ACUQPX_000853"
     CDS             41043..41879
                     /locus_tag="ACUQPX_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HHV9055635.1"
                     /translation="MKMPKRPATGSGRSHAQNNQTPISSGEFADLIPVIPGQIGRHQT
                     NVVSARALHAALNIGKDFSTWITNRISEYGFVIGSDYAVHKMISPKMGKSTNDAACSH
                     ISRTGRPGKDYLLTLNTAKEIAMVERNDRGRAIRCYFIRCEEELAHVAPQSIQKIRHQ
                     LKSRIMAASYHSPMCAALALQRAAAGKPTYPHNYATEANMIARLVLDGMTAKQWAAAN
                     GLTGDPREHMSSEQLELMAYLEQSNTTLIDTGMDYHKRKTHLARLIPQWRMRQQRNAS
                     HA"
     gene            41872..42393
                     /locus_tag="ACUQPX_000854"
     CDS             41872..42393
                     /locus_tag="ACUQPX_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HHV9055636.1"
                     /translation="MRKYCLPAIAGGRYISSAGVNPPAGRGNPFNEQKATQTRPASFF
                     VSCHRATIPSGKARTVSMVALVGQPKGWPVSFCAGISTPASVTAPYERGNSGGDSLNL
                     QKEAARMATTPTPLHPEFTFHFLAVRRSDLNDRPHRESVNAPDETTARRMLARDFVLL
                     FAGRVPSQGVRHA"
     gene            42386..42682
                     /locus_tag="ACUQPX_000855"
     CDS             42386..42682
                     /locus_tag="ACUQPX_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055637.1"
                     /translation="MRDCLRESMKAAMSSMPDEESRWSLRVDADWHRVNLLAGIAFVG
                     KALEESQLRENPITYSRDEICQLAGFLQTAPALIGCMAELMECYDQQAGEVSHV"
     gene            42675..42989
                     /locus_tag="ACUQPX_000856"
     CDS             42675..42989
                     /locus_tag="ACUQPX_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055638.1"
                     /translation="MCDMYNTRIPELLVAAIKNADAQEARALFDDADYCARKLLEGLI
                     STGRLLSGMGDNLDPSMGELRSLGDSIAVTAELVAGFGEVVEAYNWRCRTGEIREDGQ
                     HA"
     gene            42982..44025
                     /locus_tag="ACUQPX_000857"
     CDS             42982..44025
                     /locus_tag="ACUQPX_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HHV9055639.1"
                     /translation="MRDIATINAIRSVAAVAAQHAEILVPEWLPEGKRQAAEWVCRNP
                     TRADRHAGSFSVSLVDGCWHDFATGDSGGDLVALGAYLWNVRQTDAARIVADRLGLCL
                     PALGKPDVMTREQREAQRVRVQAAEQEAARLRQREQQQRHQRQKLTACRAFELLGRAE
                     VANPSHPYLMKKRLQPGNLYQSGNDLLVPLYNVCGEVVNVQIISPDGRKMFLRDGQVQ
                     GAFHVMGSFDLMAPEAPEHDVYVCEGWATGAALLQYWNVMAVVCAMNAGNLKHVAMAL
                     RERYDSRISLVIAGDDDRTSKDNPGSRAANEAALLAGCYVTFPEWPPGASPDLSDFND
                     LMLWEMEHDPDNH"
     gene            44006..45844
                     /locus_tag="ACUQPX_000858"
     CDS             44006..45844
                     /locus_tag="ACUQPX_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HHV9055640.1"
                     /translation="MTRTTTKTPVTSGKRKPPRKKHPKVSPARPGYATYSQHVQVDGG
                     QILKPGLYWHGVRDSTNTDTFICSPLEVIAMTSDSKDENYGRLLRFLTPSGQWREWAA
                     PMEMLAGDGGELRQELLRMGVTIPYKQRQALTEYIMESAPKRRALAATATGWHSPALF
                     VMPGGVIGDGDVVFQSTEAGSQEYATAGTPEGWRDNVAALCRGNPVAMLAVCASLAGP
                     LLWLLDVDSGGFHFMGDSSSGKSVGALVACSVWGEPRNFKRNWNATSTGLEGLATMRN
                     DTALILDEIGEAPARDIGGLIYQLGNGTGRQRGKVSGMARPVNTWRTMIISTGEMTVG
                     KHMESGGQRIKAGQEMRLLDIPAQRLYGAFDCLHGMALPHNPDNDTERRGAGRKFAET
                     LRGNAATHYGHAGPEFVRWLISRNESDTTSTDSNGQTLADMYAILREQFPVSTGQEAR
                     AAARFTLCALAGEMAITAGILPWKQGEALEAVKAMFDAWAGYRGRGQSEDVKILRAVA
                     GFIARHASRFEGGSTYSEPVSNRAGWWRPGSNGDRVYWFLPEALCEAASGYDLKRIAA
                     SLDDAGAIVDRDTDGKRRYTKRVSVNGEKHRVYAIGQNALLFAIDE"
     gene            46544..46834
                     /locus_tag="ACUQPX_000859"
     CDS             46544..46834
                     /locus_tag="ACUQPX_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055641.1"
                     /translation="MHKLFSLPDALCEFIRALPAPDLPEAHQPETWPEDNTTSNSQTR
                     ADMRAPSAAWLAARDAYISHVMTCPRCVTSGVKKPRHCAEGERLRQNYDHTR"
BASE COUNT        12163 a        12058 c        11543 g        11607 t
ORIGIN      
        1 cattgtcaat tggtgatgac ggtaaatacc gccaaatcat caacgtagct gatggttcag
       61 aagcccatga cgccgttacg gttcgccagt tgcagaacgc tattggagcg gtcgccacta
      121 cgccaaccaa gtactttcac gccaactcaa cggcagaaga ctcactggcc gttggtgaag
      181 actcgctggc aatgggtgcg aaaactgtcg ttaatggtaa tgcaggtatt ggtatcggtt
      241 tgaacactct ggttctggct gatgcgatca acggcattgc tatcggcagt aacgcacgcg
      301 caaaccatgc aaacagtatc gcaatgggta acggttctca gaccacccgt ggtgcacaga
      361 ctggctacac cgcctacaac atggacgcac cgcagaactc tgtaggtgag ttttctgtcg
      421 gtagtgaaga cggtcaacgt cagatcacaa acgtcgcagc tggttcagcg gataccgatg
      481 cggttaacgt gggtcagttg aaagtcactg atgagcgcgt agcgcaaaat acccagagca
      541 ttactaacct gaacaatcag gtcactaatc tggatactcg cgttactaat atcgaaaacg
      601 gtattggcga cattgtcacc accggtagca ccaagtactt caagaccaac accgatggcg
      661 tagatgccaa cgcccagggt aaagatagcg ttgctattgg ttctggttcc attgctgccg
      721 ctgacaacag cgtcgcactg ggtaccggtt ccgttgcaga ggaagaaaat acaatctctg
      781 taggttcttc tactaaccaa cgccgtatca ccaacgtagc tgccagtgtt aatgccaccg
      841 atgcggttaa cgtgtcgcag ctgaaatctt ctgaagcagg cggcgtgcgc tacgacacca
      901 aagctgatgg ttctatcgac tatagcaata tcaccctcgg tggtggcaac ggcagtacga
      961 ctcgtatcag caacgtctcc gctggcgtca acaacaacga cgcggtgaac tacgcgcagt
     1021 tgaagcaaag cgcgcaggaa acgaagcaat acaccgatca gcggatggtt gagatggata
     1081 acaaactgtc taaaactgaa agcaagttga gcggtggtat cgcttctgca atggcaatga
     1141 ccggtctgcc gcaggcttat acaccgggtg ccagcatggc ttctattggt ggcggtactt
     1201 acaacggtga atcggcagtt gctttaggtg tatcgatggt gagcgccaat ggtcgttggg
     1261 tctacaaatt acaaggtagt accaatagcc agggtgaata ctccgccgca ctcggtgccg
     1321 gtattcagtg gtaatgatcc attaagttag tgtgactaag ggcaaacgcc aaattgcctg
     1381 atgcgttacg cgcatcaggc aagataaggc gctccgggtc aacaatctaa gggcaattct
     1441 ctgatgagga ttgccctttt ctttaccaga catctccccc cacaagaatt ggccctacca
     1501 attcttcgct tatctgacct ctggttcaca attccccaat taaaactcac atcaatattg
     1561 ccaatacata acatttagtt aaccattcat tgtcattatc cctacacaac acaattggca
     1621 gcgccacttt tacacaacgt gtgacaagga gatgagcaac agactcatta cacgatgtgc
     1681 gtggactcta ggagacctgc aatgaatctc tggcaacaaa actacgatcc cgccgggaat
     1741 atctggcttt ccagtctgat agcatcgctt cccatcctgt ttttcttctt tgcgctgatt
     1801 aagctcaaac tgaaaggata cgtcgccgcc tcatggacgg tagcaatcgc ccttgccgtg
     1861 gctttgctgt tctataaaat gccggtcgct aacgcgctgg cctcggtggt ttatggcttc
     1921 ttctatggtt tgtggcccat cgcgtggatc attattgcag cagtgttcgt ctataagatc
     1981 tcggtgaaaa ccgggcagtt tgacatcatt cgctcgtcta ttctttcgat aacccctgac
     2041 cagcgtctgc aaatgctgat cgtcggtttc tgtttcggcg ctttccttga aggagccgca
     2101 ggctttggcg caccggtagc aattaccgcc gcattgctgg tcggcctggg ttttaaaccg
     2161 ctgtacgccg ccggactgtg cctgattgtt aacaccgcgc cagtggcatt tggcgcgatg
     2221 ggcattccaa tcctggttgc cggacaagta acaggcatcg acagctttga gattggccag
     2281 atggtggggc gtcagctacc gtttatgacc attatcgtgc tgttctggat catggcgatt
     2341 atggacggct ggcgcggtat caaagagacg tggcctgcgg tcgtggttgc aggcggctcg
     2401 tttgccatcg ctcagtacct tagctctaac ttcattgggc cggagctgcc ggacattatc
     2461 tcttcgctgg tatcactgct ctgcctgacg ctgttcctca aacgctggca accagttcgc
     2521 gtattccgct tcggtgactt gggggcgtca caggttgata tgacgctggc ccacaccggt
     2581 tacaccgcgg gtcaggtgtt acgtgcctgg acaccgttcc tgttcctgac cgctaccgta
     2641 acactgtgga gcatcccgcc gtttaaagcc ctgttcgcat cgggtggtgc gctgtatgag
     2701 tgggtgatca acattccggt gccgtacctc gataaactgg ttgcccgtat gccaccagtg
     2761 gtcagcgagg ctacagccta tgccgccgtg tttaagtttg actggttctc tgccaccggc
     2821 accgccattc tgtttgctgc actgctctcg attgtctggc tgaagatgaa accgtctgac
     2881 gctatcagca ccttcggcag cacgctgaaa gaactggctc tacccatcta ctccatcggc
     2941 atggtgctgg cgttcgcctt tatctcgaac tattccggac tgtcatcaac actggcactg
     3001 gcactggcgc acaccggtca tgcattcacc ttcttctcgc cgttcctcgg ctggctgggg
     3061 gtattcctga ccgggtcgga tacctcatct aacgccctgt tcgccgcgct gcaagccacc
     3121 gcagcacaac aaattggcgt ctctgatctg ttgctggttg ccgccaatac caccggtggc
     3181 gtcaccggta agatgatctc cccgcaatct atcgctatcg cctgtgcggc ggtaggcctg
     3241 gtgggcaaag agtctgattt gttccgcttt actgtcaaac acagcctgat cttcacctgt
     3301 atggtgggcg tgatcaccac gcttcaggct tatgtcttaa cgtggatgat tccttaatga
     3361 ttgttttacc cagacgcctg tcagacgagg ttgccgatcg tgtgcgggcg ctgattgatg
     3421 aaaaaaacct ggaagcgggc atgaagttgc ccgctgagcg ccaactggcg atgcaactcg
     3481 gcgtatcacg taattcacta cgcgaggcgc tggcaaaact ggtgagcgaa ggcgtgctgc
     3541 tcagtcgacg cggcggcggg acgtttattc gctggcgtca tgacacatgg tcggagcaaa
     3601 acatcgtcca gccgctaaaa acgctgatgg ccgatgatcc ggattacagt ttcgatattc
     3661 tggaagcccg ctacgccatt gaagccagca ccgcatggca tgcggcaatg cgcgccacac
     3721 ctggcgacaa agaaaagatt cagctttgct ttgaagcaac gctaagtgaa gacccggata
     3781 tcgcctcaca agcggacgtt cgttttcatc tggcgattgc cgaagcctca cataacatcg
     3841 tgctgctgca aaccatgcgc ggtttcttcg atgtcctgca atcctcagtg aagcatagcc
     3901 gtcagcggat gtatctggtg ccaccggttt tttcacaact gaccgaacaa catcaggctg
     3961 tcattgacgc catttttgct ggtgatgccg acggggcgcg taaagcaatg atggcgcacc
     4021 ttagttttgt tcacaccacc atgaaacgat tcgatgaaga tcaggctcgc cacgcacgga
     4081 ttacccgcct gcccggtgag cataatgagc attcgaggga gaaaaacgca tgattatttc
     4141 cgcagccagc gattatcgcg ccgcagcgca acgcattctg ccgccgttcc tgttccacta
     4201 tatggatggt ggtgcatatt ctgaatacac gctgcgccgc aacgtggaag atttgtcaga
     4261 agtggcactg cgccagcgta ttctgaaaaa catgtccgac ttaagcctgg aaacgacgct
     4321 gtttaatgag aaattgtcga tgccggtagc actggcaccg gtgggtttgt gtggcatgta
     4381 tgcgcgtcgt ggcgaagttc aggcagccaa agcggcggac gcgcatggca ttccgtttac
     4441 tctctcgacg gtttccgttt gcccgattga agaagtcgcg ccagccatca agcgcccaat
     4501 gtggttccag ctttatgtac tgcgcgatcg cggctttatg cgtaacgcgc tggagcgagc
     4561 aaaagcagcg ggttgttcga cgctggtttt caccgtggat atgccgacac cgggcgcacg
     4621 ctaccgtgat gcgcattcag gtatgagcgg cccaaatgcg gcaatgcgcc gctacttgca
     4681 ggcggtgacg catccgcaat gggcgtggga tgtgggcctg aacggtcgtc cacatgattt
     4741 aggtaatatc tcagcttatc tcggcaaacc gaccggactg gaagattaca tcggctggct
     4801 ggggaataac ttcgatccgt ccatctcatg gaaagacctt gagtggatcc gcgatttctg
     4861 ggatggcccg atggtgatca aagggatcct cgatccggaa gatgcgcgcg atgcagtacg
     4921 ttttggtgct gatggaattg tggtttctaa ccacggtggc cgccagctgg acggtgtact
     4981 ctcttccgct cgtgcgttgc ccgctattgc agatgcggtg aaaggtgata tcgccattct
     5041 ggcggatagc ggaattcgta acgggcttga tgtcgtgcgt atgattgcgc tcggtgccga
     5101 caccgtactg ctgggtcgtg ctttcctgta tgcgctggca acagcgggcc aggcgggtgt
     5161 agctaacctg ctaaatctga tcgaaaaaga gatgaaagtg gcgatgacgc tgactggcgc
     5221 gaaatcgatc agtgaaatta cgcaagattc gctggtgcag gggctgggta aagagttgcc
     5281 tacggcactg gctccgatgg cgaaagggaa tgcggcatag tcgtttgcgc cccctcaccc
     5341 taaccctctc cctcagggag aggggaccgt tcggcgctgt atgtactccc tcactctgaa
     5401 acgacaccgc actctttttt tctccctcgc ccctccgggg agggccgggg tgaggggaaa
     5461 aggccgcacg gttcaccaat tttctgctat cctgcccccg cactaagggg gcagtatgct
     5521 aaacatcgta ctttacgaac cagaaattcc gccaaatact ggcaacatca tccgtctttg
     5581 cgctaatacc ggctttcgtc tacatatcat cgaaccgatg ggatttgcct gggacgataa
     5641 gcgcctgcgc cgcgcggggc tggactatca cgagtttacc gccgttacgc gtcatcatga
     5701 ctatcgcgcg ttcctcgaag cagaaaatcc ccagcgcctg tttgccctca ccacgaaagg
     5761 tacgcctgct cacagcgccg taagctatca ggatggcgat tatctgatgt tcggcccgga
     5821 aacacgcggc ctgccagcga gcattcttga tgccctgccc gctgaacaaa aaattcgcat
     5881 tccgatggtg ccggacagcc gcagcatgaa tctgtccaat gcggtgtcgg tagtggtgta
     5941 tgaagcctgg cggcagctgg gatatccggg agcgttgttg agagattagt tactgtatgc
     6001 cggatagatg attacatcgc atccggcacg atcacaggac attagatccc atccccatac
     6061 tcaaatgtat ggttaatacc gttgaaatgc tggtccatat ccattgatgg cttatcgctg
     6121 tctggtttac cgacaatacg agccggaacg ccagcggcgg ttgtatgcgg cggcaccggt
     6181 tgcagcacca cggaacctgc gccaatcttc gcgccgcgac caacttcaat attgccgagg
     6241 attttcgcgc ccgcgccaat catcacacct tcacgaattt tcgggtgacg gtcaccacca
     6301 gatttacccg taccgccaag cgtcacagat tgcagaatcg atacgtcgtt ttcaattacc
     6361 gccgtttcac ccacaacgat gcccgtggcg tggtcgagca taatgccacg gccaattttt
     6421 gctgccgggt gaatatcgac ctggaacgtc acagaaacct ggttttgcag aaagattgcc
     6481 aatgcgcgac gcccctgatt ccacaaccag tgaccgatgc gataggcctg caaggcatga
     6541 aaacccttca ggtataacaa cggggttgag tatttatcga ctgccgggtc gcgggtacgc
     6601 accgcctgaa tatcacaggc cgcagaggcg atcatttccg ggtcagcggc gtaggcttct
     6661 tcaaccactt cacggatagc aatagcaggc ataattggcg atgacagctt gttcgccagc
     6721 atgtagctca gtgcactgcc aaggttttcg tgcttgagta gcgtcgcgtg gtaaaaactg
     6781 gccagcattg gctcacagtc cgccagcgtt ctggcttcgg ctttaatatt gttccagaca
     6841 atttccagtt cttcacacga cattgcttac tccacacgat gagataatga ccggcccgtt
     6901 ctgcgcgggc cgggtcatag cggtaacaaa ggttccctgg ggttagtggc tgctgcgctc
     6961 gtccttgcgt gcacgaccta ataatgtcaa tgctgcctcg cgcgcgtttt ttccgcaata
     7021 taatacttga taaatttcct cggttattgg catttcaacg ccgaagcgat gcgccagttc
     7081 gcggacttct ttcgtattgc ggtagccttc caccacctga ccaatcttct cctgcgcgct
     7141 ttgtacatcc atgccctgac cgagcatcat gccaaaacgg cggttacgcg actggttgtc
     7201 ggtacaggta agcaccagat cgcccagccc cgccatgccc ataaaggtgg caggatcggc
     7261 acccagcgcc gcaccaagac gcgacatttc agccagccca cgggtgatca gcgccgtacg
     7321 cgcattcgca ccaaaaccga taccgtcaga catccccgcg ccaatggcaa tgacgttttt
     7381 caccgcgccg ccaagctgca cgccaatgaa atccgggttg ctgtaaacgc ggaaactttt
     7441 gccgcagtgc agcagctgct ggagatcatc ggcaaaggtc tgatcggtcg aggccagcga
     7501 aatagctgtc ggtaaacctg ccgccagttc tttcgcaaac gttgggccag agataaccgc
     7561 cagcggaatt tgatcgccta aggcctcacg cgccacgtcc tgtaacagac gtccggtttc
     7621 cgcttccagc cctttggtcg cccacaccag acgcgcatca ggacgcatca gcggtttaat
     7681 ctggcgcagc acttcaccaa agacatggct gggtacgacg acgagaatat tacgactggc
     7741 tgccagcgca gtggcgagat cgctttcaag atggagcgta tcgggaaaag gcacatcggg
     7801 gagaaacgcg gcgttacagc ggtcgcgttc aagcgttgcg atatgttcag ggtcatggcc
     7861 ccagaggaca acctcgtggc catttcttgc cagggtaatg gcaagagcgg tgccgtacga
     7921 gccggcaccg atcacagtca ttgaagcatt acgttggttc atcaggcatc ctgatgttct
     7981 tcagtacctt cgccagcctg ctgctgcaaa tagttcatga acagcgcatc gaagttaacc
     8041 ggcgcaaggt tcagttgcgg gaatgtaccg cgggatacca tgctggtgat gcactcacga
     8101 gcatacggga acagaatgtt cgggcagtat gcgcccaggc aatgtgccat ctgggtgcct
     8161 tcgatacccg cgatggagaa aataccgccc tgctgaactt cacacaggaa cgcggtttct
     8221 tcgcccaagg aggccgttac ggtaacacgc agtaccactt cgtatacgtc atctgccagt
     8281 tgggaagatg ccgtatccag atcaagttta acttctggtt gccaatcttt ctggaaaacg
     8341 tgcggcgcgt tcggcgcttc gaaagagata tccttggtat aaatacgttg gatctggaaa
     8401 gtcatttcag tgttgttttg ttctgacatg tgtagaaaac ccttaagtgt tgtccttaaa
     8461 tactgcgcaa tgtaatgcca acgttcacac gttatttcag caggggatcc agtccaccac
     8521 gtgcatccaa tgcatacaag tcatcacagc cgccaatgtg ctgtgcgtca ataaaaatct
     8581 ggggaaccgt ggtgcgaccg ctgcgtttga tcatctcttc acgcttggcg gcattgccat
     8641 cgatcggcaa ctcctggaaa ctcacgccct tgctgctcag cagtgctttt gcacgatggc
     8701 aatacgggca ggtttctttg gtatagattt caacattggc cataatttag ctcctgtttt
     8761 tttaccctga aatttcatgt tgcagggcgg ctgcaaaatg gagtgtcccc agaagcttat
     8821 aacaataagt cgctgggggc ctaattgcag ctaacggcct gcatcatgaa agacgacagg
     8881 taaattattt gccgcgcacc agaggcaagt tttcgccagc ccagccagca acgccctctt
     8941 tcagcacgaa tacttgcgca aaaccagctt tcgtcagcgc gtttgcaggc tcctggcact
     9001 gcatgccaga accgtctacc acgataaccg gtttgtcttt gtgcttctca agctcaccaa
     9061 cattgttggc tttgatttcg ctcggcaaca gattgataga acctgcgata tggcctttac
     9121 ggaagtcatc acgctgacgt aaatccacaa ccacagcgtc ttctttgttg atcagacgcg
     9181 tagcttcacc acgagtaatc accttcactt tcgaggtcag gctcttaaac gtagtcacaa
     9241 gaaccgccac cagtaacgcg atccaggcga tactcagtat gggatgacgg ccaacaaatt
     9301 gcataatttc ttgcatgggg ggtaacaact cccgacgtag tgattaaaaa aaccaggaaa
     9361 ggagtatacc tgcgcggtgc ggcaaataca gccagcgcgt taactggaat gcaattttgc
     9421 ggggcacgac gaaaaaaaag cgcaaatctg gcatcggctt gccgcgagcg gtcgtatatt
     9481 ttgatcttca gaggctattt tatcgattca gctgtagtaa aattacgcaa attttgactc
     9541 ttgagtatga ggttgtcgca atgtcggttt ctaaaaaacc tatggtactg gtgattctgg
     9601 atggctatgg ctatcgtgaa gaacagcagg ataacgccat ttttagtgct aaaaccccgg
     9661 taatggatgc actgtgggcc aatcgtccgc ataccctgat cgacgcttcc ggtctggaag
     9721 tcggtctgcc tgaccgtcag atgggtaact ccgaagtagg ccacgttaac ctgggtgccg
     9781 gccgcatcgt gtatcaggac ctgactcgtc tggacgttga aatcaaagat cgcgctttct
     9841 ttgctaatcc agtgctggct ggcgcggtac ataaagcgaa aaacgcaggt aaagcagtac
     9901 acattatggg tctgctctcc gcaggcggcg tacacagcca cgaagatcac atcatggcga
     9961 tggtagaact ggcagctgaa cgcggcgcag aaaaaatcta cctgcacgca ttccttgacg
    10021 gtcgcgacac cccgccgcgc agtgctgaat cctcgctgaa aaaatttgaa gagaaatttg
    10081 ctgcgctggg taaaggccgc gtagcgtcca tcattggtcg ttactacgcg atggaccgcg
    10141 ataaccgttg ggatcgcgtt gaaaaagcct atgacctgct gactctggcg cagggtgagt
    10201 tccaggccga tactgccgtt gctggtttgc aggcagctta tgctcgcgac gaaaacgacg
    10261 agttcgtgaa agcgaccgtt attcgtgcag aaggtcagcc agatgcggca atggaagacg
    10321 gcgatgcgct gattttcatg aacttccgtg ctgaccgcgc gcgtgaaatc actcgtgcat
    10381 tcgtgaacgc tgatttcgat ggcttcgcgc gtaagaaagt ggttaacgtc gatttcgtga
    10441 tgctgaccga atacgccgct gacatcaaaa ctgcggttgc ttacccaccc gcttccctgg
    10501 ttaacacctt cggcgagtgg atggcgaaaa acgacaaaac tcagttgcgt atttccgaaa
    10561 ccgaaaaata tgcccacgtt actttcttct tcaacggtgg cgtagaagag tcgttcaaag
    10621 gcgaagatcg cattctgatc aactcaccga aagtggctac ctacgatctg caaccggaaa
    10681 tgagctccgc agagctgacc gaaaaactgg ttgcggccat caagagcggc aaatacgaca
    10741 ccattatctg taactatccg aacggcgaca tggtaggtca caccggggtg atggaagcgg
    10801 cggttaaagc agttgaagca ctggatcact gcgtggaaga agtcgcgaaa gcggttgagt
    10861 ccgtgggtgg acaactgctg atcaccgctg accacggtaa cgctgaacag atgcgcgatc
    10921 cggcaacggg tcaggcacac acggcacaca ccaacctgcc agttccgctg atttacgttg
    10981 gtgataagaa cgtgaaagcg gttgcaggcg gcaaactttc tgacatcgcg ccgaccatgt
    11041 tgtcgctgat gggtatggaa atcccgcaag agatgactgg taagccgctg ttcatcgtgg
    11101 aataatccct ccccatgagg ggaaaggcga ttaataccat gacacgggcc gtgaaaccgc
    11161 gcaggtttgc aatcaggccc atcatctacg ccagcgttct tagcgctggc gtattgttgt
    11221 gcgccttttc cgcccacgcg gatgagcgtg accaactcaa atctattcag gccgatatcg
    11281 ccgcaaaaga gcgcgcggta cgccaaaagc aacaacaacg cgcaagcctg ctcgcacaat
    11341 tgaaaaagca ggaagaagcg atctctgaag ccacccgtaa gctgcgcgaa acgcaaaaca
    11401 cgctcaatca actcaataaa cagattgatg agatgaacgc gtcgattgcc aaactggagc
    11461 agcaaaaagc cgcccaggag cgcagcctcg ccgcacaact ggatgccgca ttccgtcagg
    11521 gcgagcatac cggtattcag ctaattctca gcggtgaaga aagccagcgt ggacagcgtt
    11581 tacaggctta tttcggctat ctcaaccagg cgcgacaaga aaccattgcc cagttgaagc
    11641 aaacgcgtga agaagtcacc atgcagcgtg ctgaactgga agagaaacaa agcgagcaac
    11701 aaacgctgtt atatgaacag cgcgcccaac aggcgaaact gactcaggct ctgaacgatc
    11761 gtaaaaagac gctggcgggg ctggaatctt ccatccagca aggtcagcag cagctaagtg
    11821 aactgcgtgc caacgaatcc cgtctgcgta acagcattgc ccgtgcggaa gccgcggcga
    11881 aagcgcgtgc tgaacgagaa gcgcgtgagg cccaggcggt tcgcgagcgc cagaaagaag
    11941 cgacgcgcaa aggcaccacc tacaaaccga ccgaaagcga aaaatcgctg atgtcccgaa
    12001 ctggtggcct gggtgcgccg cgtggtcagg cattctggcc ggttcgcggg ccgacgctgc
    12061 atcgctatgg cgagcagcta cagggtgaat tacgctggaa agggatggtt atcggtgcct
    12121 ctgaaggtac tgaagttaaa gcaattgccg acggtcgagt gattctggct gactggctgc
    12181 aaggttacgg tctggtggtg gtggttgagc acggtaaagg cgacatgagt ctttacggct
    12241 ataatcagag cgcactggtg agcgttggtt cgcaggttcg cgcgggccag ccaattgcac
    12301 tggtgggcag cagtggcggt cagggtcggc cttcactcta tttcgaaatt cgccgccagg
    12361 gtcaggcggt caatccacag ccgtggttgg gaagataagt tttgtttcca tttcgtcgta
    12421 acgttcttgc atttgccgct ctgttggcgc tctcctcccc cgtacttgct ggcaaacttg
    12481 ccatcgtcat tgatgatttt gggtatcgcc cgcacaacga aaaccaggtg ctggcgatgc
    12541 cttccgctat ctccgtcgct gtattacccg attcaccgca cgccagagag atggcgacca
    12601 aagcgcataa cagcggtcac gaagtgttga ttcatctccc gatggcaccg ttgagtaaac
    12661 agccgctgga gaaaaatacg ctacgcccgg agatgagcag cgacgaaatt gagcgcatta
    12721 ttcgtagtgc ggtcaataac gtgccctatg ccgtggggat caacaaccac atgggcagca
    12781 agatgacctc taacctgttt ggtatgcaga aagtgatgca ggcgctggag cgttacaatc
    12841 tttacttcct cgacagcgta accatcggta atacccaggc gatgcgcgcc gcgcaaggta
    12901 ctggcgtgaa ggtgatcaaa cggaaggtgt tcctcgacga ttcgcagaac gaagcggaca
    12961 tccgtgtgca atttaatcgc gcaattgatc tggcgcgtcg caacggttca accattgcca
    13021 ttggacatcc tcacccttca acggtacgcg tgctacaaca gatggtttat aacctgccgc
    13081 cagacattac gctggtgaaa gccagcagct tgctgaatga accgcaggtt gatacttcta
    13141 caccgccgaa aaacgctgtg cctgatgcac cgcgtaatcc gttccgtggc gtgaagctgt
    13201 gcaaaccgaa gaaaccgata gaacctgttt atgctaatcg cttctttgaa gtattaagcg
    13261 aaagcatcag ccagagcacg ctgatcgttt acttccagca tcagtggcaa ggctggggca
    13321 aacagcccga agcggcgaag tttaacgcta gcgcaaatta aaggcgctac gcgtaatgcg
    13381 atggtgcgac cgcgttttgt cgcgccattg ccataagcgg aaagaccaca gtaacgcctg
    13441 gtaaccgacc ttcacgctgc gcacattggt aatcaggcgc ttatacatac cggaagtaaa
    13501 tatctcggca atcatccgtt ggcgggtaag aatatccggc tctttgcgca ccgcatggca
    13561 aacacgcaat gcttcgtaag ttatttgctg atgaaattcc ggataaatcg taattttgtc
    13621 ggcataattt cgatttaatt tctccagcag gcgggtaatc ttaatatagt gacgttgata
    13681 attaaggttt ttattccctt gtctatgcaa ccgactcact gacgtattat gcagataata
    13741 tttatataat tattgctcgg tatatcgcgc acgcagcgcg ttaaacatga attctgttgt
    13801 ccagacaata tcctgatgat gtaatccggc aataaattta atgttatttt taacaataac
    13861 atcacggcga taaaccccca tccagacaac gtgagtccaa cggcgcgaag aaagccccat
    13921 ccgcagccag tccgggccgg ttaatacacc ggttgagcga aggcgatcgc tggggatgga
    13981 ttgccaagtt tctcccgttt cacgaaaaga ccagtcagcg ttgcactgcg ccacgtcgag
    14041 gtcgtcctct aacgccatgg tcatcagcgt ttcgtacatg gtgggataga cctcatcgtc
    14101 agcatcgaca aaagcgacat atttgcccgt cgccacctcg atcccacgat tacgcgctac
    14161 cgatgtgcca gcattcgcct gatgcaacaa acgaacgtgc ggatagtttt ctgcgtaatg
    14221 ctttgctatt tcaacagaat tatccgttga accatcgtta ataataatga tttccagagc
    14281 agtccaggtt tgcgtaatta aagattccat acaagtgcgg aaatcatcgc ccgcattata
    14341 taacggaata ataacactaa gtttattggt gctgttcatc ataaattacc gacgacctct
    14401 gaagttatcc tgacgtttta caggctttgt attaagaaac tattaagcct gtgagatatt
    14461 atttttacag gtgagagagt cacttcaggg tagagagata atgagaacag ctatataaaa
    14521 gatcagcgca attacgaact ctttattaca ctgacaggat tttacataat taacagatta
    14581 tcctcagata aaagggctgg aataccagcc cttgttcgtg ttaatcccag ctcagaataa
    14641 ctttcccgga ctggcccgaa cgcatagcgt caaagccctt ctggaaatca tcgatagaga
    14701 aacgatgggt aatgatcggc gagagatcga ggccagactg aatcagcgcc gccatcttgt
    14761 accaggtttc aaacatctca cgaccgtaaa tgcctttaat gaacaagcct ttaaagatca
    14821 ctttggtcca gtcgatagac atatcagacg gtggaatacc cagcatcgca atacgaccgc
    14881 cgtggttcat ggtatcgagc atggtacgaa acgctggcgg cgcgccggac atttccagac
    14941 cgacatcaaa gccttcggtc atgcccagtt cagccatcac atcattaagg ttttctttcg
    15001 cgacgttaac cgcacgggtg atacccattt tgcgcgccag ctcaaggcgg tattcgttaa
    15061 catcggtgat caccacattg cgtgcaccaa cgtgtttcgc caccgccgca gccataatac
    15121 ctatcggacc tgcaccagaa accagcacat cttcgcccac cagatcgaac gacagcgcgg
    15181 tatgcacggc gttaccgaag ggatcaaaaa tggaagccag gtcgtcggag atattgtcgg
    15241 ggattttgaa agcgttgaac gccggaatca ccagatattc ggcgaagcag cccggacggt
    15301 taacaccgac accaatggtg ttgcggcaca gatgggtacg accgccacga cagttgcgac
    15361 agtgaccgca ggtgatatgg ccttcgccag aaacgcgatc gccgattttg aagcctttca
    15421 cttcctgacc aatacctacc acttcaccga catattcatg gcccacgacc atcggcaccg
    15481 ggatggtttt ttgcgaccac tcatcccagt tatagatgtg aacgtcagtc ccgcagatgg
    15541 ctgttttacg gattttaatc agcagatcgt tatgcccgag ttccggtaca gggacgtcgg
    15601 tcatccagat gccctcttcc gctttcagtt tggataacgc tttcatctca catcctcagg
    15661 cgataacgcc cagttgttta ccaatacgcg tgaatgcttc tactgcacgc gtaatttgct
    15721 caggggtatg cgccgcagac atctgggtac gaatacgcgc ctgacctttc ggaacgaccg
    15781 gatagaagaa accggtgacg taaatgccct ctttttgcag ctctcgggca aatttctgcg
    15841 ccactaccgc atcaccaagc atgaccggaa taatggcgtg atcggctccc gccagggtaa
    15901 agcccgccgc cgacatttgt tcacggaact gacgcgcgtt cgcccacaga cggtcacgca
    15961 actcgctacc cgcttcaacc atctccagca ctttgatgga agcggcgaca atcgctggtg
    16021 ccagcgagtt ggagaacagg tacggacgag aacgctggcg cagccactca accacttctt
    16081 tgcgcgccgc ggtataacca ccagaagccc cgcccagcgc tttaccgagc gtgccggtga
    16141 tgatgtcgac acgccccatc acatcgcagt attcatggga accacgacca ttttcaccga
    16201 caaaaccgac cgcgtgggag tcgtctacca tcaccagggc atcgtattta tctgccagat
    16261 cgcaaacgcc cttcagattg gcaatcacgc cgtccatcga gaacacacca tcggtggcaa
    16321 tcagcacatg acgcgcaccg gcttcacgcg cttctttcag acgagcttca agctcctgca
    16381 tatcgttgtt ggcatagcga tagcgtttag ctttacacag acgcacacca tcaataatag
    16441 acgcgtggtt cagtgcgtcg gagataatgg cgtcttccgc gcccagcagc gtttcaaaca
    16501 ggccaccgtt agcatcaaag caggaagagt agagaatcgc atcttccatc cccaggaatg
    16561 ccgccagttt ttgttcaagc tctttatggc tgtcctgagt gccgcaaata aaacgcaccg
    16621 aagccatgcc gaaaccgtga gaatccattc ccgcctttgc tgccgcaatc agatccggat
    16681 gattcgccag cccgagatag ttgttggcac aaaagttaat gacgtggctt ccatcagcca
    16741 cggtgatatc tgcttgctgc gcagacgtaa taatgcgctc ttctttaaac aacccttccg
    16801 cccgtgcggt ttccagatcg ttggttaact gctgataaaa ttctccacgc attgcgattc
    16861 tccagacagg gcaaattcca gcacatatta cccaaactta taggtcgcga cgagatgatg
    16921 cgttaacact tctgcaaaat tcaggataaa taacgtgtat cttgatgtct ctcaacacct
    16981 acagatagta gaaaaactgt acagttttgg ctgagcataa tgatgagtga ttttactgca
    17041 ttgatataaa aagaaaaaat gatgccaaag catcattttt cctatctcag ttggcatcaa
    17101 aacgctaccc actaatggag ggaaacctat tttcttgaaa ggaaaattct cagcgcctgc
    17161 atttttgccc ctaaagtgtt actcctgaag cagcgaaaca aatcgaacca ttttcctctc
    17221 cccagatagt ttaggttgaa gagatcggaa cgtttgtaat agcacatgac aaaaatgccc
    17281 tggtcatcgt ccacaatgtt attgtttagt gttatttttt gactttccag aactaattta
    17341 tagaattctt tccatttatg ctgtgaacca acaattgcgc ccccaataat atatacatga
    17401 ttgccaatca tgaaatcaaa agcctgttgt tgagatgtaa cagtaagccc tttttttata
    17461 gtgaaaagat gcatcttatt ttcatcaaat gggaaatccc aaattttcaa tcctcgagtt
    17521 acatttggct tacggcaata gccaaaatct atccacgcaa caagaggcgt tttcactaaa
    17581 cccatgttga tagctttatt tacaaaatag gccttaaggt tacataccaa aacatactct
    17641 ggtgaccagt actctgggtt ttttaattgt cgaggctcaa gtctatttgt aaacgactca
    17701 tctttttgaa ttttttcgat acgacttctg atatatctaa atttcttttt tatatcgata
    17761 acaataaccg ttgttggttt tccgttgcga atcgcttcaa tccttggttt caaatcggga
    17821 gaagtgaaaa taatcatctc attttcaagt gcggccagac gctcaaagta actaaaataa
    17881 acatcaactg aacgagctag tttttcacgg aaccctttat tagcagtcca atcccctcta
    17941 ccaatgtcaa aatatgcagt gataattgtg gtggatgatt tcattactaa taagaccatt
    18001 aatataaaaa agaattatat cataaatatt aacagaatgt atactgtata ttatccattc
    18061 aaatgattcc gttatttatc atacatcgga atattgatac taaagcacta ttcacatgca
    18121 aaaccaacat ccgccatgaa ggactagcta aaacccaaac tagtttgttg caattagcat
    18181 ccttgcacct ctatgtaaag ggctgaaggg attcggatgt gatggtatga ttacagacat
    18241 tcgtgtctga gattgtctct gactccataa ttcgaaggtt acagttatga tcatcgttac
    18301 cggcggcgcg ggctttatcg gcagcaacat cgttaaggcc ctgaatgata aaggcatcac
    18361 cgatattctg gtggtggaca acctgaaaga cggcaccaag tttgtgaacc tggtggatct
    18421 ggatatcgcg gactatatgg ataaggaaga cttcctgatc cagattatgg ctggcgaaga
    18481 gttcggtgat gtcgaagcga ttttccacga aggcgcatgc tcttccacca ccgagtggga
    18541 tggcaagtat atgatggata acaactatca atactccaaa gagctgctgc actactgtct
    18601 ggagcgcgaa atcccgttcc tgtatgcctc ttccgcagcc acctacggcg gacgcacctc
    18661 cgactttatt gaatcccgcg agtacgaaaa accgttgaat gtctacggtt actcaaaatt
    18721 cctgtttgat gaatatgttc gtcaaatcct gccagaagcg aactcgcaga ttgttggctt
    18781 ccgctatttc aacgtttatg gaccgcgtga aggccataaa ggcagcatgg cgagcgtcgc
    18841 tttccatctc aacacccagc ttaataacgg tgaatcaccg aagctgttcg aaggtagcga
    18901 gaacttcaaa cgtgacttcg tctatgtggg cgacgtggca gatgtaaacc tgtggttcct
    18961 ggaaaatggc gtttccggta tcttcaacct cggcaccggt cgtgcagaat ccttccaggc
    19021 agtagcagat gctacgctgg cttatcacaa gaaaggccag atcgaataca ttccgttccc
    19081 ggataagctg aaaggccgct accaggcgtt cactcaggca gatctgacaa atctgcgcgc
    19141 ggcgggttac gacaaaccgt tcaaaaccgt tgctgaaggt gtaacggaat acatggcctg
    19201 gctgaatcgc gacgcataag agctctgcat gaaaatactg gtgatcggcc cgtcttgggt
    19261 tggcgacatg atgatgtcgc aaagtctcta tcgcacgctc caggcgcgct atccccaggc
    19321 gataatcgat gtgatggcac cggcatggtg ccgtccatta ttatcgcgga tgccggaagt
    19381 taacgaagct atccctatgc ctctcggtca cggagcgctg gaaatcggcg aacgccgcaa
    19441 actgggtcat agcctgcgtg aaaagcgcta cgaccgcgcc tacgtcttac caaactcctt
    19501 caaatctgca ttagtgcctt tcttcgcggg tattcctcat cgcaccggct ggcgcggcga
    19561 gatgcgctac ggtttactca acgatgtacg cgtgctcgat aaagaaacct ggccgctaat
    19621 ggtggaacgc tatgtcgcgc tggcctatga caaaggcatt atgcgtaccg cacaagatct
    19681 gccgcagcca ttgttatggc cgcagttgca ggtgagcgaa ggtgaaaaat catatacctg
    19741 taatcaattt tcgctttcat cagaacgtcc gatgattggc ttttgcccgg gtgcggagtt
    19801 tggtccggca aaacgctggc cacactacca ctatgcggaa ctggcaaagc agctgattga
    19861 tgaaggttat caggtggttc tgtttggctc tgcgaaagat catgaagcgg gcaatgagat
    19921 tcttgccgct ttgaataccg agcagcaggc atggtgtcgg aacctggcgg gggaaacaca
    19981 gcttgatcaa gcggttatcc tgattgcagc ctgtaaagcc attgtcacta acgattctgg
    20041 cctgatgcac gttgcggcgg cgctcaatcg tccgctggtt gccctgtatg gtccaagtag
    20101 cccggacttc acaccgccgc tatcccataa agcgcgcgtg atccgtctga ttaccggcta
    20161 tcacaaagtg cgtaaaggtg acgctgcgga gggttatcac cagagcttga tcgacattac
    20221 tccccagcgc gtactggaag aactcaacgc gctattgtta caagaggaag cctgacggat
    20281 gcgggttttg atcgttaaaa catcgtcgat gggcgatgtt ctccatacgt tgcccgcact
    20341 cactgatgcc cagcaggcaa tcccagggat taagtttgac tgggtggtgg aagaaggggt
    20401 cgcacagatt ccttcctggc acgctgccgt tgagcgagtt attcctgtgg caatacgtcg
    20461 ctggcgtaaa gcctggttct cggcccccat aaaagctgaa cgcaaagcgt ttcgtgaagc
    20521 gctacaagca gagaactatg acgcagttat cgacgctcag gggctggtaa aaagcgcggc
    20581 actggtgacg cgtctggcgc atggcgtaaa gcatggattg gactggcaaa ccgctcgcga
    20641 acctttagcc agcctgtttt acaattgtaa gcatcatatt gcaaaacagc agcacgccgt
    20701 agaacgcacc cgcgaactgt ttgccaaaag tttgggctat agcaaaccgc aaacccaggg
    20761 cgattatgct atcgcacagc attttctgac gaacctgcct acagatgctg gcgaatatgc
    20821 cgtatttctt catgcgacga cccgtgatga taaacactgg ccggaagaac actggcgaga
    20881 attgattggt ttactggctg attcaggaat acggattaaa cttccgtggg gcgcgccgca
    20941 tgaggaagaa cgggcgaaac gactggcgga aggatttgct tatgttgaag tattgccgaa
    21001 gatgagtctg gaaggcgttg cccgcgtgct ggccggggct aaatttgtag tgtcggtgga
    21061 tacggggtta agccatttaa cggcggcact ggatagaccc aatatcacgg tttatggacc
    21121 aaccgatccg agattaattg gtgggtatgg gaagaatcaa gtagagtgtc gttctacaag
    21181 tatgtctctt gcagatttgc cagctcaaac cgtttttcaa aatttaaatc ttgaaataat
    21241 aaccaataag ttgacatcgg agataagatg acctcaacat tatttttctc tctcgagaaa
    21301 aaaaactgga tagcgtactg gaacagagct ctcgtattct tattcattac cacctatttt
    21361 ttgggtggga ttacaaggta taaacatctt attgttattc ttatgacaat aacgacaatc
    21421 gtctatctct gcaaacggcc aaaacactat ctctcactat ttaaaacatt tctttttggt
    21481 agtgttgcca tattaactat tgctgcattg ctgtcacttc ttcaatcccc tgatgcaggt
    21541 gctagcatga aggaaatttt caaagctatt attgagaata ctttactatg cacaatagca
    21601 ataccggtca tattgagaga cgagaaaaga gaagatgtcg aaaaaatcgt tttcttctca
    21661 tttattagtg cgttgggctt acgctgtttt tctgaattga ttacctatta taaggactat
    21721 caacaaggga taatgccatt cgcagattat agacaccgta gcatttctga ctcgatggtc
    21781 tttttattcc ctgcattgtt aaatctctgg cttatcaaat cagcaaaata ccgcatttct
    21841 tttgtggttc taagcgttat ttttattttt ctgatattag gaactttatc cagaggggcc
    21901 tggctttccg tgttagtcat tggattaata tggattctga tgtttaaaca atggaagtta
    21961 ctattagtag gagtaatggt ttccatcatt gcattgtcgg ttattttcac acataaggag
    22021 atgaccgcaa agctaacgta taaacttcaa caaactaata gttcttatcg ctatgcaaat
    22081 ggtactcaag gcagcgcact cgatctaata ttagaaaatc ctgttattgg ttatggttac
    22141 ggtaacgttg catataaaga tgtctataat aaacgtgtca ttgattatcc agaatggacc
    22201 tttagacaat caatagggcc acataatttt gcgctattca tctggtttgg cactggttta
    22261 ttagggctgg taagtcttat gatgctatac tgtgcaatat tgaaagagtg tataaaaaat
    22321 ggcgtcaaga ataaatatcg ctcaccatat aatgcatatt atataatctt actatctttt
    22381 ataggttatt ttgttatccg tggaaacgta gaacaaattg aaccaaattt attaggcgtt
    22441 tacgccggct tattattagc gatgaaaaac aagtaaaaaa taaaaaaggc tgcataatgc
    22501 ggccttttta tttattcaaa ccaattatga ataacctctt cgaatctttg agttacgcca
    22561 tcccaactgt agtgatcaaa cacaaagtcc tgaccttgct ttgcaactgc tgttaattca
    22621 ggattcgcta acgtttttaa aatatcgctg cttatggagt ctgcagtcat agattctttt
    22681 aaatggaatc ccgtagtatt ttctttgaca aattccgtca tccccccacg cgtactcact
    22741 aaaacaggct taccagcacc catagcttca attgccacca tgcaaaatgg ttcctggaac
    22801 tgggaaggaa tgacgactaa atctgccagt gggtaataac aatgcatctt ttcaggcggt
    22861 atgcttccta acataatgca acgatccttc aaacgttttg caatttccct gacattgcgt
    22921 tgatatgcac ctttgtcact tttactcatt tctgtataat caccaatcac aactaatttt
    22981 aaatttttgt gagcagcggc caatttttcg aatgattgca tcaacagcaa tatgcctttg
    23041 tcagggacga tacgcccagc ataaaaaata attttctctt caggggagat attaagttcg
    23101 cttctagtga tcggctgaaa attagattga taggtttcta aatcaattcc atttggtaca
    23161 atctcaatat ctgcatttgc taaataagat tggtaatact ttttcaagta taaactaggg
    23221 actatcattt taacattttg ctcaagcaat tttggttcaa atgcattatg catatgtata
    23281 accactcttg cctgaggagc tcgcttacga atttgagtgt atagcttcat gctattatga
    23341 actacaataa cactttcttt cgttatagga aaatcatgtg caatattcaa tattctctgt
    23401 gaatatggaa gaggatcaag tcttgtccat ttttgaaata accgcttata tatgcgactg
    23461 aatccaaccc tatgaatact acaattatca ttaacaaagg tgtaatttga gtatcctgga
    23521 tttttgatac aaacaatacg atttggaaaa ttggtacgct gagcaacctg atacatccac
    23581 gtttcaacag cagccgctcc gcgaggagga attgaaaaaa taggagtaac cgtaaatatt
    23641 attttatcaa ccatctcgta tattaacctt tcataacatt atatttaatt gctgtaaaaa
    23701 caccatcgag aaattttttc tggtaaagca agtgtttata tttttctttg tgctcatgtt
    23761 ttttaacagc ttttttataa ggtatatttc tccatggtga tatattataa atattacgaa
    23821 aataatctgc agaggcgtag ttcgcccaat catgccatgg cttagttata ccagtataat
    23881 gtatgaagac agtgtcatca ttaataaacc gggtgtaata ttcactattt ttttcttcaa
    23941 attctgactt tatagtataa atacaattat attttctcgg taatatttta gcatgatgta
    24001 gaaacattaa gtttaaaata tcttgatccg ggtactttaa tttttttata attgcctcat
    24061 ctgataatag atcaaagaat ttttccgtta gtctttgttt taaccattcc cgtaaattaa
    24121 tatacattac accagagtta aaatagctac cgttaaaatc ctcattacac aaacgctcag
    24181 cgcttttact ttgcatagca tctacatcaa ttaccactgc cccatattca tctttaaact
    24241 ctaatgctat taactcgttc aatgaaccct tacagacgat atcagcatca agatataata
    24301 atgaatccaa tcgcgcagaa aaataatcaa atgaaagcaa acgataataa atagatactg
    24361 gccagatatt tgattgaggt agctccttca atggctcaga atcaatatga tataatttaa
    24421 ttactgtttg atacttcgtt gctaattgtg ataataattc aatatactct ttgtctaagt
    24481 aatcagcgat tatgtgaaag gtaaatttgt tttgtttatt attttcaaga attgaagcaa
    24541 ttgaaatcgc agccccaacc tggtagttct catcaatacc ccatgagaca ttaaacgttt
    24601 cccgttcatc aagtttaacg gggcgcttgt ccagttcaat tatatcttta aattgagtaa
    24661 gatgtttaaa atccaattta attacccttc atcataatca ttatttgctc ttcaatccta
    24721 gtttgacttt tacatccctg attacttttt taattttctt tttgaatata agatatgtat
    24781 agccaaagtc tttcaattca ttttttatat tatagtgccg ttcaagatca atgcgatctt
    24841 ttgcttttaa aacagcggtt gtttttttac ctgaaaggtc aattagtctt aaaccttttt
    24901 cggatactat aaaatttcct ttatgaggat cgccagacac cattccatgc tgatgcaact
    24961 cttttatcga ttctgataat tgatccttca gatcttcaga aatttccaaa tactcattta
    25021 acccgacacc ttcaatatac tcaatgagca taatataata atgagcgaaa tttaatgttt
    25081 tacgttcagc tagcaaaaaa tagtcattaa ttgattggat gccttcacca cgtacccgat
    25141 cggtttgata aattaaattt tcataataat ctttttttat gcaagatttt aagaaacgtt
    25201 cggtcatttt atgctttgcc gcataaactt taagtacaag aggtccacga gcagtgtcaa
    25261 taagaatgac tttagtatca tcaatagaac gaaaaacttt taatgtttta accctgcaag
    25321 ttaaaaagtc atttagaact tgctcataaa agggatcatt atcttttatg aaaactttca
    25381 acccatttat cgttttatta tatatcataa tgaattattt taaccttaaa tctttagaag
    25441 catttttctt tataatactt taaataatta aaaatgcctg ccatataatg cttttgttta
    25501 aatttatgtt ttgcacaata ccgatattga ttgctattta caggtttaag taaatcttct
    25561 ttactccacg gagaagctgc tttggcgatc aaaaaactac gtgaaaccgg ataatcagcc
    25621 cactcatgcc agggttttgt aggtcccaca tagtgtataa acacggtgtc atcattgact
    25681 ggattatcaa ctttgtcttt taattcatag ttaatactat atcgggtatt atatttctcc
    25741 gaaataaact tcactttacc attcaataat acattcagta catcttgatc aaggtgggtg
    25801 attttactta cccaatctgg gtcacgcaac atttcaatag cttttgacga aatgttattt
    25861 agattccact catcaatatt tatcagtaaa aaaccagcat tgaaatatcc agaagctaac
    25921 tgtggtgtag ttaataccga ggctcgattt tgccaccatt caatatctct ttcagctaca
    25981 actgcggcaa tttcattagt agaaaattga taatctaaga gttctttaat actacccttg
    26041 caagcaatat ctgcatcaag atatagtatt ttttcatgtt tatgatagaa ataatctgca
    26101 attataaatc gaaaatatgt tgcgtaagtc cagtttttcg tgcttggtaa tgacttcagc
    26161 ttatcacaat tgataacata aatattaatt cttgaattat attgttttgc taaatcagaa
    26221 aaatataatt tgtctttatc actgatataa tctgtgaaaa catgaaattc acaagagatt
    26281 tctctattgt ttaatagaat agatgcgatg gctacaccac atccaaaaag aaaatttctg
    26341 tcaataccaa atgctatatc aagagtattt ttagatctta tattttgata atgaaaatta
    26401 tactcaaaaa ggatgatgtc actatcattg agttgagaca tatttattct gtatccaaaa
    26461 ttttggcaaa aataagttac gggttacaac gattttcgaa tcgttctttc cctgattttt
    26521 gtggcttttg cttctgcttg cgacagcagt ccttgttcct gcttgagaat gtctttaagc
    26581 ggggcggcaa aatacacctt cataaagcgc cagatatccc gctgagtcag gccaatattc
    26641 attgaagaaa aataaagccc tataagatct ttatcgcgcc aacgacgtgg aacgcgcgtg
    26701 cgaagctgcg cccggtgtag gtcaattacc gaaattttta actcctcttc cttaccggaa
    26761 aaaggcaagt gcagcaggaa atgacagata taacagtcac gatggttaat gcccgcagca
    26821 tgcatatcgc gcaccatcgt cgcgacacgc ttaataagca tacgctttac gcgaacatct
    26881 ggtgggttgg tcgcccagtc agcacagtaa tcttccagac tgatggttgg tgtcagatct
    26941 tcggtaataa taaacgaggt tctggtcagc ggattcatgc ctttttcgcc gaatgccacc
    27001 ccatacatag tatcaacgcc gacatcccgc agtcgatgaa tcgcattcca ttcgcggtct
    27061 gcgcctaata ctggcatccg caatgagagt aaatttttga ttatctcttt cagggtcgtg
    27121 ccgcgatgcc atttgagaaa atagcttttt cccgccattt caaagcgcag agtacggcga
    27181 gtttccagtt cacgaaatac ctcaccctgc aaggttttaa cttcctcaaa aggatctttg
    27241 ccgcgccata acgtggcaaa cggctcttta agttcaacca tccagaccac ccgttatgat
    27301 atccgccgct ttctctggca gactgtataa atcttgtgta tcagcataat gtcgcgcatt
    27361 ttccgcccaa gcctggcgca atgaagattg cgttaacgct ttgcgtaaaa tctcattcaa
    27421 tgtttcctgg cggaatggct cagcaatagc ctcgccgcaa ttagcgtcta caatataatg
    27481 cgcatagcca caaacggcag ttgttagtac cggtaatcct gcagtaatcg cttccagcag
    27541 cacaattccc gccgcttcct ggtaggcagg atgcagtaat aaatccgccg ccgccattaa
    27601 ttccgagaca tcgttgcgcc ccgagaagaa gtgaacatta ctgcgcacgc cgcgtttttc
    27661 tgccagtgcc tcaaattttc gcggtttatc ctgcccaaca acatatagca atgtgttgtg
    27721 gcgcagcgaa tccggtaacg aagcaagtgc ttcaatggaa cgatcgacac ctttacgcgt
    27781 gaagtctgaa ccgacctgca acaataaata ttgttgttcg gttattccat tcttcttacg
    27841 gaagatttca cggctattgg ctggctgctg gctatattta cgatcaggat aaatccccgg
    27901 tggcagaata tgaaaacgct ccgcttcagt ctgataatgt ttctggaaat cggcgatttg
    27961 cttatctgtc agcatcagca gctgtgtcgg cttgctctgt tcgaaggttg cccgctcaaa
    28021 ggcggcataa tggcgataac gtgacgtcag gcgatagaaa aagccttttt cctgcgctac
    28081 tttctcggca taacaaacat cagcggcata ataaacgtcc agccccggca ttttgttgaa
    28141 tccaacgact ttatcgacgg gatgttcgcg taaatgtttt tgcacccagg caaaatactc
    28201 cgcattgcgc ccgtgattgg tatgcgattt aaccggcact ttgatcagtt caaatacatc
    28261 agggcattcg ccttcccacg actgggtata aacccgaaca tgatgacctc gggcggcgac
    28321 tgtctgagca atacgcataa aatcgcgctg caaaccgcca aagggaaaat atttatataa
    28381 acaaaaagca acgatcataa cgacgtacct gtcgtggagg agggcagtaa tttatcgaca
    28441 gcagcaatga catctgccgc cggaataacc gaaagataca tctcatttcg gtcacgctga
    28501 tcgcgcgttg gcatttcccg gtaatctccc gcccagaatt gaatcatgtt atttgaccag
    28561 ggacgccaga aaatatgatc tgttgcgcca aacagcgata tcagcggcgt attaactgca
    28621 gcggcaatat gcgccggtgc ggaatcaacg ccaataaaca gctgcgcatg atcgattaac
    28681 gcaccaagtt ccgggaaggt cacctttcca gccagcgccg ttactggtgg cgtctggcat
    28741 ccctgcgcaa tttcattgac gcaggccaga tcgtctttat ctgggccgga cgtcagaacg
    28801 acttcataac cacgagcatg taaggcatca atcacagcgg aaaacttggc gttgtcccag
    28861 catttgaaga tttgccgcgc cgtaggttgg ataaccacat aattttgtcc aacaccagcg
    28921 tgatcaagtt cgcgacgcat acgtttccag cttgcaggcg ggtaactcat ggttgtctgc
    28981 ttcaccaacg aatcaagtcc caatggtgtc agcacggata agttactttc caccacattt
    29041 ccaccctgca acggcaccaa atgggtgaaa cttttacgcc aaaaagcaga ctgccgatga
    29101 tgataatcct gggaaatttt cacacgagca tttaataagc gaaccagtat agcaaccatc
    29161 cattgatcgg taagattgac gataaggtca tacttattgg cacgtaatac cttgatgaga
    29221 tgaaaaaagt tggcaatttt ttctgaggct tttgcttttt tattttttat gccgtagagc
    29281 gcgttaatct ctggattttc agacaggatc gggatggtgt cctgataaag cagcacatcg
    29341 atttttgcgt caaggtaatt ttttttcagc gaactaatga cgggagtagt taataacata
    29401 tccccatgaa aacgcatctt aatgagcaaa attcttcgaa atggcttatc cacaagtaac
    29461 tctttttgtt gagattatcc gattaaggtg aagcagaaaa agcacgctac cgccccaggc
    29521 tcaacagcta cctgaatact gataccgcac tgatattgac gcgctagtgt atcacttctt
    29581 tcgcggcaat ccgacccgaa tgatttttaa gcacattgtt gattttcaat aaagaaatca
    29641 atcaaataaa atcattagca accatattct ttcactgttg ctctgcgatt ccttcctgca
    29701 cgtacagcag aaatcaatga atcgcataaa aatacaaaaa aagtatagat gttcagcaag
    29761 aattggcggg taaattgcta tttaagtcaa aaacgggaaa agtaatggta aagccacagc
    29821 taaatacata gaatccccag cacatccata agtcagctat ttactatgct cgaattgctt
    29881 tacaccgccc ttctctacct tattcagccg ctgatctgga tacggctctg ggtgcgcgga
    29941 cgtaaggctc cggcctatcg aaaacgctgg ggtgaacgtt acggttttta ccgccatccg
    30001 ctaaaaccag gcggcattat gctgcactcc gtctccgtcg gtgaaactct ggcggcgatc
    30061 ccgttggtgc gcgcgctgcg tcatcgttat cctgatttac cgattaccgt tacaaccatg
    30121 acgccaaccg gttcggagcg cgtacaatcg gctttcggga aggatgttca gcacgtttat
    30181 ctgccgtatg acctgcccga tgcgctcaac cgtttcctga ataaagtcga ccccaaactg
    30241 gtgttgatta tggaaaccga actatggcct aacctgattg cggcgctaca taaacgtaaa
    30301 attccgctgg tgatcgctaa cgcgcgactc tctgcccgct cggccgcagg ttatgccaaa
    30361 ctgggtaaat tcgtccgccg cttgctgcgt cgtattacgc tgattgctgc gcaaaatgaa
    30421 gaagatggtg cacgttttgt ggcgctgggc gcaaaaaata accaggtaac cgttaccggt
    30481 agcctgaaat tcgatatttc tgtaactccg cagttggctg ctaaagccgt gacgctacgc
    30541 cgccaatggg caccacaccg cccggtatgg attgccacca gcactcacga aggtgaagag
    30601 agcgtggtca ttgccgcaca tcaggcattg ttacagcaat tcccgaattt attgctcatc
    30661 ctggtacccc gtcatccaga acgcttcccg gatgcgatta accttgtccg ccaggctgga
    30721 ctaagctata tcacacgctc ttcaggggaa gtcccctcca ccagcacgca ggttgtggtt
    30781 ggcgatacga tgggcgagtt gatgttactg tacggcattg ccgatctcgc ctttgttggc
    30841 ggttcactgg ttgaacgtgg tgggcataat ccgctggaag ctgccgcaca cgctattccg
    30901 gtattgatgg ggccgcatac ttttaacttt aaagacattt gcgcgcggct ggagcaggca
    30961 agcgggctta ttaccgttac cgatgccact acgcttgcaa aagaggtttc ctctttactc
    31021 accgacgccg tttaccgtag tttctatggc cgtcatgccg ttgaagtact gtatcaaaac
    31081 cagggcgcgc tacagcgtct gcttcaactg ctggaacctt acctgccacc gaaaacgcat
    31141 tgaggttgtt atgcaaaaac gggcgattta tccgggtact ttcgatccca ttaccaatgg
    31201 tcatatcgat atcgtgacgc gcgccacgca gatgttcgat cacgttattc tggcgattgc
    31261 cgccagcccc agtaaaaaac cgatgtttac cctggaagag cgtgtggcac tggcacagca
    31321 ggcaaccgcg catctgggga acgtggaagt ggtcgggttt agtgatttga tggcgaactt
    31381 cgcccgtaat caacacgcta cggtgctgat tcgtggcctg cgtgcggtgg cggattttga
    31441 atatgaaatg cagctggcgc atatgaatcg ccacttaatg ccggaactgg aaagtgtgtt
    31501 tctgatgccg tcgaaagagt ggtcgtttat ctcttcatcg ttggtgaaag aggtggcgcg
    31561 ccatcagggc gatgtcaccc atttcctgcc ggagaatgtc catcaggcgc tgatggcaaa
    31621 attagcgtag cgtttatgcc ggatggtatg ccatccggct cgcatgaatt acttctggca
    31681 ctgccgacaa taaaacgttg cccgctgcgc atgtttagtc gccacaatcg gcgtaccgca
    31741 cacccgacac ggctcacctt ttcgcccgta aacctgcaat tcctgcgcga aatagcccgg
    31801 tttaccatca ctttgcagaa aatctttcag cgttgtacca ccctgctcaa tcgaacgcag
    31861 caacaccgct ttaatcaccc gagctaacaa ttcacactct gccagcgaca gtgatgacgc
    31921 cagccgatcc ggatggatcc ccgccgcaaa cagtgattcg ctggcataga tattccctac
    31981 ccctaccacc agcttgttat ccatcagcca cggtttaatc gccgtttttt tcttcgcgca
    32041 cttctgatgc agatactcac cattgaaatc gtcgctaagc ggctccggtc caagatgggc
    32101 cagcacatta tgcccttcca gctctttggt ccacagccag gcaccaaagc ggcgcggatc
    32161 ggtgtagcgc agcactttac cgttgctcat caccaaatcc acatggtcat gcttttcagg
    32221 gggaagttct tctggaagga tgcgcaggct gccagacatc cccaaatgaa tgataatcca
    32281 gccctcaggc agctccagca gcagatattt agcccgccgc tgcacgctaa gcactggttg
    32341 atcgcttaat cggtagatct cttctgaaac cggccagcgc aagcgtccgt tgcgcaccac
    32401 cgcatgaaga atggttgcac caacgagatg cggttctatg ccgcgacggc tggtttcaac
    32461 ttcgggtaat tcaggcatag catctccagg aatgaacaga tgcaatcaat atgggggcaa
    32521 gcagataaca aaaaaccccg ccggagcgag gtttttcgtt acatcaaagc gagaattatt
    32581 tgattttcgc ttctttgtag atcacgtgct gacgaacaac tggatcgaat tttttcagtt
    32641 ccagtttttc cggcttagta cgtttgttct tcgtagtggt atagaagtga ccagtaccag
    32701 cagaagaaac cagcttgatt ttctcacgaa tacctttagc catgatttat ttcctctaag
    32761 tacttagtac ttttcgccac gggcacgcag ttcagccaga actgtatcga tgcctttttt
    32821 atcgattaca cgcatacctt tagcagatac gcgcagggtg acaaaacgct tctcgctctc
    32881 aacccagaaa cggtgagagt gcaggttcgg caggaaacgg cgtttagtcg cgttcagtgc
    32941 gtgggaacgg ttgttaccgg tcaccggacg cttgccagta acttggcaga ctcgggacat
    33001 gtctattctc caaaaatcaa attagctcga gcttcgtatg gggtattggc gcctcgtcag
    33061 gctttacagc ccggtcatcg cagttctatg tgaactctcg attgccaggc ccaaatgcca
    33121 aacccgagat tctcaaaggt ggcgtagtat acgctgactc agcgatgtgc tcaagtcccg
    33181 aacagacaaa gatcccgaag gatcgcgcat agcgggttaa atccagccgc gttcggcaaa
    33241 agaaacatac tctccacgcc caatcacgat atggtcgagc acgcgtaaat ccatgaactg
    33301 acaactcttt attatccgtt cagtaatgag tttatccgct ttactgggtt cagcacaacc
    33361 cgaagggtga ttatgtgcaa ggatcagcgc cgaggcgttt atttttatcg cttcgcggat
    33421 aatttcccga ggatggactt caacatggtt tagcgtgccg gaaaaaagac ggctatgcgt
    33481 tataacccgg tgttgtgagt cgagaaagat caccataaag atctcccgct cctcacccgt
    33541 gagctggctt tgtaaaaatt cccgcgtcat ctccgggctg agtaaagggc tttcttcacg
    33601 catccgcacg ttgtagtaac gtcgcgccag ttcagcaatc ccctttaact gggcaaattt
    33661 cgctacgcca attccatgaa caccactaaa ttgttcatat tcagaggtta acaagccata
    33721 aagagagccg aaattctcca gcatctcttt tgccagggtt aatacatctt taccgcgcgt
    33781 tccggtacgc agaaatagcg ccagcagctc gacatccgtt aaggcgctaa taccaaactt
    33841 cagcattttt tcgcgcggca tcaacagctg tgcattgttt ttcaccttca cctcctttgt
    33901 ggtgctcgca tcctgtcata gccgttccgt taaatcgacg gccagttttc aatcctggaa
    33961 agagcctcgc aaagtgaatc tcaggtgaag gcacgacaac aaaagtattg tgataaaatc
    34021 gccaacttct ggtgtcaccc aacaggaaaa atcatcatga gcctggccgg taaaaaaatc
    34081 gttctcggcg ttagcggcgg tattgctgcc tataaaaccc ctgaactggt gcgtcgtttg
    34141 cgcgatcgcg gggccgacgt ccgcgtagcc atgaccgaag cggcaaaagc ctttatcacc
    34201 ccacttagct tgcaggcggt ttctggttat cccgtttccg acagtctgct tgacccggca
    34261 gccgaagccg ctatgggcca tattgagctg ggtaaatggg ctgatttagt gattctcgcc
    34321 cctgccacgg cagatttgat tgcccgtgtt gctgccggaa tggcgaatga cttggtatcg
    34381 acgatttgtc tggctacacc tgcgcctgta gccgtgctcc ccgccatgaa ccagcagatg
    34441 taccgtgccg ctgccacgca gcataattta gaggtgcttg cttcccgtgg tttgctcatc
    34501 tgggggccag acagtggcag tcaggcttgt ggtgatatcg gtcctgggcg aatgctcgat
    34561 ccgttaacca ttgtggatat ggcggtggcg catttttcgc ccgtcaacga cctgaaacat
    34621 ctgaacatta tgattaccgc cggtccgacg cgtgaaccgc tcgatccggt gcgttatatc
    34681 tctaatcaca gctccggcaa gatgggtttt gctatcgccg ccgctgctgc ccgtcgtggc
    34741 gcgaacgtca cgctggtatc aggtccagtt tcactaccga cgccaccgtt tgttaaacgt
    34801 gttgatgtga tgaccgcgct ggaaatggaa gccgccgtga atgcttctgt acagcagcaa
    34861 aatattttta tcggttgcgc cgccgtggcg gattatcgcg cagctaccgt ggccccagaa
    34921 aaaatcaaaa agcaggccac gcagggtgat gaattaacaa taaaaatggt taaaaacccc
    34981 gatatcgtcg caggcgttgc cgcactaaaa gaccatcgac cctacgtcgt tggatttgcc
    35041 gccgaaacaa ataatgtgga agaatacgcc cggcaaaaac gtatccgtaa aaaccttgat
    35101 ctgatctgcg cgaacgatgt ttcccagcca actcaaggat ttaacagcga caacaacgca
    35161 ttacaccttt tctggcagga cggagataaa gtcttaccgc ttgagcgcaa agagctcctt
    35221 ggccaattat tactcgacga gatcgtgacc cgttatgatg aaaaaaatcg acgttaagat
    35281 tctggacccg cgcgttggga aggaatttcc gctcccgact tatgccacct ctggctctgc
    35341 cggacttgac ctgcgtgcct gtctcgacga cgccgtagaa ctggctccgg gtgacactac
    35401 gctggttccg accgggctgg cgattcatat tgccgatcct tcactggcgg caatgatgct
    35461 gccgcgctcc ggattgggac ataagcacgg tatcgtgctt ggtaacctgg taggattgat
    35521 cgattccgac tatcagggcc agttgatgat ttccgtgtgg aaccgtggtc aggacagctt
    35581 caccattcaa cctggcgaac gcatcgccca gatgattttt gttccggtag tacaggctga
    35641 atttaatctg gtggaagatt tcgacgccac cgaccgcggt gaaggcggct ttggtcactc
    35701 tggtcgtcag taacacatac gcatccgaat aacgtcataa catagccgca aacatttcgt
    35761 ttgcggtcat agcgtgggtg ccgcctggca agtgcttatt ttcaggggta ttttgtaaca
    35821 tggcagaaaa acaaactgcg aaaaggaacc gtcgcgagga aatacttcag tctctggcgc
    35881 tgatgctgga atccagcgat ggaagccaac gtatcacgac ggcaaaactg gccgcctctg
    35941 tcggcgtttc cgaagcggca ctgtatcgcc acttccccag taagacccgc atgttcgata
    36001 gcctgattga gtttatcgaa gatagcctga ttactcgcat caacctgatt ctgaaagatg
    36061 agaaagacac cacagcgcgc ctgcgtctga ttgtgttgct gcttctcggt tttggtgagc
    36121 gtaatcctgg cctgacccgc atcctcactg gtcatgcgct aatgtttgaa caggatcgcc
    36181 tgcaagggcg catcaaccag ctgttcgagc gtattgaagc gcagctgcgc caggtattgc
    36241 gtgaaaagag aatgcgtgag ggtgaaggtt acaccaccga tgaaaccctg ctggcaagcc
    36301 agatcctggc cttctgtgaa ggtatgctgt cacgttttgt ccgcagcgaa tttaaatacc
    36361 gcccgacgga tgattttgac gcccgctggc cgctaattgc cgcgcagttg cagtaatatg
    36421 acgccggatg acttttcatc cggcgagttt ctttaaacgc caaactcttc gcgataggcc
    36481 ttaaccgccg ccagatgttc cgccatttcc ggcttctctt ccagataggc aatcagatct
    36541 ttcagggtga tgatagagat cactttgcag ttgtaatcac gctcaacttc ctgaatcgcc
    36601 gaaatctcgc cgcgcccgcg ttcctgacga tcgagcgaaa tcaacacgcc agcaagcgtc
    36661 gcgccattgg cctgaataat ctccatcgac tcgcgaatcg ccgttccggc ggtgatcaca
    36721 tcatctacca gcattacgcg tccttgtaac gcgctaccaa ccagattgcc gccttcaccg
    36781 tggtcttttg cttctttgcg gttaaagcag tacggcaggt ccaggtcgtg atgctccgcc
    36841 agtgccacag cggttgtggt ggcaatcggg atccctttgt aagcagggcc aaacagcaga
    36901 tcgaactcaa tgccggaatc caccaacgct tcagcgtaaa aacggcctaa cagtgccaga
    36961 tcgcgcccgg tattaaacag cccggcgttg aagaaatagg ggcttttgcg cccggatttc
    37021 agcgtaaact cgccaaactt taacacctgc ttgctaagcg caaattcaat aaactggcgc
    37081 tgatatggtt tcatgccttc gctcctcatc ttacttttct acagacaaaa aaaaggcgac
    37141 tcatcagtcg ccttaaaaat cagtttgcca gcgccgcctt ctgcgtcgct acaatggatt
    37201 cgattccccc tcgggccagc gccaacaagg tgagtagctc ttcatgggtg aacggctcgc
    37261 cttctgccgt cccctgcact tcaatgatgc gcccgtcttc ggtcatcact acgttcatgt
    37321 cggtctctgc ggctgagtct tcaacgtatt ccagatcgca aattgcttca ccgtttacaa
    37381 taccgacaga aactgcggct accatccctt tcatcgggtt ggttttcagc ttgccgtttt
    37441 ccaccagctt ctgtagcgca tctgccagcg ccacgcaggc acccgtaatc gacgcggtac
    37501 gagtgccacc atcagcctga agcacgtcgc agtccagcgt aatggtgaac tcacccagcg
    37561 ctttcaaatc tactgccgcg cgaagagcac gggcgatcag acgctggatt tccattgtgc
    37621 gtccaccctg cttacctttc gccgcttcac gagcgttacg ggtgtgggta gaacgtggca
    37681 gcatgccgta ctctgcggtg atccagccct ggccctgacc tttcaggaag cgcggcacgc
    37741 cttcttcaat agaggcggta cacaatactt tggtatcgcc aaattcgacc agcaccgagc
    37801 cttctgcatg ttttgtatag ttacgagtca gggtaacggg acgcacctga ttattgctac
    37861 ggcctgctgg acgcatattg aaatctccgg cttgaaacaa atgtggctgc gcattatacg
    37921 gacttccggc ggttattcct atcctgacaa ggcatcgatg gctataatcc ttccacctct
    37981 ccttttataa acaggaacgt ctatgatccg cagtatgacc gcctacgccc ggcgtgaaat
    38041 caagggtgaa tgggggagcg caacctggga aatgcgctcg gtaaaccagc gttatctgga
    38101 aacttacttt cgtctgccgg agcagttccg tagccttgaa cctgtcgttc gcgagcgtat
    38161 tcgttctcgc ctgacgcgcg gtaaagtgga atgtaccctg cgctatgagc cagatgttag
    38221 cgcacaaggt gagctgatcc tcaatgaaaa actggctaaa cagctggtaa ctgccgcgaa
    38281 ctgggtaaaa atgcagagtg acgaagggga aatcaacccg gttgatattc tacgctggcc
    38341 gggcgtgatg gcagcccagg agcaggatct cgacgcgatt gccgctgaaa ttctcgcggc
    38401 gttggatggt acgctggacg actttatcgt cgcgcgcgaa accgaaggtc aggcactgaa
    38461 agcattgatc gagcagcgtc tggaaggcgt caccgccgaa gtggtcaaag tccgcgccca
    38521 tatgccggaa attatgcaat ggcagcgtga gcgtctggtc gcgaagctgg aagatgctca
    38581 ggttcagttg gaaaacaacc gtctggagca ggagttggtt ctgctggcac aacgaattga
    38641 cgttgccgaa gagctggacc gcctcgaagc gcatgttaaa gagacgtaca acattctgaa
    38701 gaaaaaagaa gcggttggtc gtcgtctgga ctttatgatg caggagttca accgtgagtc
    38761 gaacactctt gcgtcgaaat ctatcaatgc cgaagtgaca aactccgcca tcgagctgaa
    38821 agtgttgatt gagcagatgc gcgagcagat tcagaacgtg gaataacccc cagccaactc
    38881 cgaggcactc cacacaaaaa cagcaaagcc ccattctatg gggctttttt actgtaatgc
    38941 actccgatat actccaaagc aatccagcaa aaaccgtgta catacacgtg tacatacggg
    39001 atttatggac acatgtttat gtacacaccc ctattcaata ttcaggtgga gttatggcaa
    39061 aggcgaggct aacggatatc cagataaggg catggataaa ggccggagaa cactttgagg
    39121 gacgcgccga tggcaacggt ctgtatttgt gcttccctga aaaatacagc gccccattct
    39181 ggcgttatcg ctaccggatg gccgggaaac aacgcacgat gcacatcggc tcatattccg
    39241 acctgtcact aagtaaagcc cgtgccaccg tcaaagaact ggccgcccgt gttgcgctgg
    39301 gtcatgacgt ggccggagaa aagcaggccc ggaaagcggc ggcactggca cggattgagg
    39361 aagaaaaaaa cgcccttcgc gtgtcagaac tggcagccga atactacgaa cggcaaatac
    39421 taccccgctg gaaacatccc gatattctcc gccgccgcat aaacaaagat atcaaccctc
    39481 agattggcag cctgaaagtc gaagatgtta aaccgcgcca tattgatgca atgctacagc
    39541 gcattattga ccgtggagcg ccaacagttg ccaacgatgt tttgcgatgg actaaacgga
    39601 tattcgacta cgccatcaaa cgccacgcac tggaaacaaa ccccgccgct gcttttgaat
    39661 tatcagatgc tggtggggct gaaaaaagcc gcgaccgatg gctaacccgt gatgaactca
    39721 ttcgtttttt tcaggccatg cgcacagcta aaggattcag ccgtgagaat gaactcacca
    39781 tgaaactgat actttgcctt tgttgccgaa aaatggagtt gtgcgccgcg cagtgggatg
    39841 aatttgattt agatagtgct gtatggcgac tccccgccga acgggctaaa aacggggatg
    39901 ccattgatat cccactacca ccaccagcag tggcatggtt gcgtgaatta cacagcattt
    39961 caggcggtag ccgctgggtg cttccagccc gcaaaatgca acagcgcatg attccacaca
    40021 tacaggaaag caccctgccc gttgcgctgg ctaaagttcg cggggaaatg cctggtgtgc
    40081 ctggttttac cgttcacgat ttccgacgca cggccagaac tcaccttgcc gcgcttgggg
    40141 tagaccctgt tgtggcggaa cgctgcctga accaccggat taaaggcgtt gagggtatct
    40201 ataaccgaca tcagtatttc aatgaacgcc gccacgctct ggaattatgg gccgacctgc
    40261 tggtggctct ggaacagggg agcgaataca acgtaacccc atttaaaata gccaaataac
    40321 gcgcacaatt gattgtgcgt taatttaacg gattaataat acccctacag gcatgaacac
    40381 atttaaacgc gtataaaggt ttttaaacgg gtttttaacg gggttaagca aaaagtatat
    40441 ttttgaaagt catttgacac caattttgat ggttatttct ctgccaatgc aaatttttag
    40501 caattcgtgc ggaataaaac accacaaaac gctaaatata aagggcaata agccatgttg
    40561 cacaaatgtt gattctttct gcggctgata agagcgcatg agagaatttt gcggttaaca
    40621 aataccccca tagctatttc ccctataaaa ctcatataaa gatttttaaa cgtgttttta
    40681 acgatgtcac catccccact cctgccggat aaaaacccag aaaataccgc taaaacaaag
    40741 tggtattgcc tttctacggt gcatagttat aggcttttta tcactttcaa tcgctttatt
    40801 cagggggcaa taaaatggac acattaccta aatctggcta tatgcgccca actcaggtga
    40861 tgcgccactt ccaaatcagc gaatccacgt tatggaactg ggttaaaaag ggtaaattcc
    40921 ctcgcccgga taaactcagc gacggcgtta gtcgctttga tgtttccgct atccgtaagt
    40981 ggcttgcaga ccgcaaaggc acaaccagca cacgtaatta attccgtcag agacttttaa
    41041 tcatgaaaat gccaaaaagg cccgcaacag ggtcagggcg ttctcacgcc caaaacaacc
    41101 agacacccat ttcatcaggt gaatttgccg acctgattcc tgttattccg gggcaaattg
    41161 gccgccatca aactaacgtt gtaagtgcac gtgcgctaca tgctgcgctg aatatcggga
    41221 aggatttttc gacatggata accaacagaa taagtgaata tggttttgtt attggcagtg
    41281 attacgccgt gcataaaatg atttccccaa aaatggggaa aagcacgaat gatgcggcct
    41341 gtagccatat ttcacgtact ggccgccccg gcaaagatta tttactcacg ctgaacaccg
    41401 ccaaagaaat agcgatggtg gaacgtaacg accgtggccg ggccattcgt tgttatttca
    41461 tccgttgcga agaagaactg gcacacgtag cgccgcaatc catacagaaa attcgccacc
    41521 agctaaaaag ccggataatg gcagccagtt atcacagtcc catgtgcgcc gcactggctt
    41581 tacagcgtgc cgctgccgga aaacccacct accctcataa ctacgcaaca gaggccaata
    41641 tgatagcccg gcttgtactg gatggaatga cagccaaaca atgggctgcg gccaacggtt
    41701 taaccggaga tccacgcgag cacatgagta gcgaacagct tgaactcatg gcctaccttg
    41761 agcaaagcaa cacaactctg attgatacgg ggatggacta ccacaagcgc aaaacgcatc
    41821 ttgcccggct tattcctcaa tggcgaatga gacaacagag gaacgccagc catgcgtaaa
    41881 tattgcttgc ctgccatcgc tggtgggcgt tatatttcct ccgctggtgt aaatccacca
    41941 gccgggcgtg gaaacccgtt taatgagcaa aaggcgacac agacgcgccc agcgtctttt
    42001 tttgtgtcat gccatcgcgc aaccataccc agcggcaagg cccgcactgt atctatggtg
    42061 gcgctggtgg ggcagccgaa aggctggccg gtttcctttt gcgccggtat ttccaccccc
    42121 gccagcgtca ccgcccctta tgagcgtgga aactcaggcg gtgactcctt aaatttgcaa
    42181 aaggaggctg ccagaatggc tacgacccca accccgttac accctgaatt tacctttcac
    42241 tttctggccg ttcgccgttc cgacctgaac gaccgcccac accgcgaatc cgtcaacgca
    42301 cccgacgaaa caaccgcccg ccgtatgctg gcgcgtgatt ttgtgttgct gtttgctggc
    42361 cgcgttccgt ctcagggggt gcgccatgcg tgattgtctg cgtgagtcaa tgaaagctgc
    42421 catgagcagt atgccggacg aagaaagccg ctggagtctg cgcgttgatg ccgactggca
    42481 cagagtgaat ttactggcgg ggattgcctt tgttggtaag gcgctggagg aaagccagtt
    42541 acgcgaaaac cccatcacct attccagaga cgagatctgc cagttagccg gatttctaca
    42601 gacagcccct gctctcattg gctgtatggc cgaactgatg gagtgttacg accagcaggc
    42661 cggggaggta agccatgtgt gatatgtata acacccggat acctgaacta ctggttgcgg
    42721 ccatcaaaaa cgcagacgca caggaagccc gcgccctgtt tgatgatgcg gattactgcg
    42781 cccgcaaact gcttgaaggg ctgattagca caggccgcct gctttccggc atgggcgaca
    42841 atctcgaccc cagcatgggc gaactacgca gcctgggcga ttccatcgct gttacggctg
    42901 aacttgtggc cggattcggt gaggttgtgg aggcgtataa ctggcgctgc cgcaccgggg
    42961 aaatcaggga ggacgggcaa catgcgtgac atagccacga ttaacgcaat ccgcagcgtt
    43021 gccgcagtgg cggcacaaca tgcggaaatc ctcgttcctg aatggctgcc cgaaggtaaa
    43081 cggcaggccg ctgaatgggt gtgtcgtaac cccacacgtg cagaccgtca cgcgggcagt
    43141 ttttccgtct cgctggtgga cggatgctgg cacgacttcg ccaccgggga cagcggcggc
    43201 gaccttgtgg cgctgggggc ttatttgtgg aatgtgcgtc agactgatgc cgcccgtatt
    43261 gtggcggaca ggctggggct gtgcctgccc gcgctgggta aacctgatgt aatgacccgc
    43321 gaacaacggg aggcgcaacg ggtgcgggta caggccgcag aacaggaggc ggccagactc
    43381 cgccagcggg agcagcaaca gcgccaccag cgccagaaac tgaccgcctg ccgggccttt
    43441 gagttgcttg gccgggcgga ggtggcaaac ccttctcatc cctatctcat gaaaaaacgg
    43501 ctacagcccg gcaatctgta tcagtcgggt aatgatttgc ttgtcccgct gtacaacgta
    43561 tgcggcgaag tggtgaacgt gcagattatc agcccggacg gtcgaaaaat gtttctccgt
    43621 gatggtcagg tgcagggggc tttccatgtt atggggagct ttgacctgat ggccccggaa
    43681 gcgccggaac atgacgttta cgtgtgtgag gggtgggcca ccggggccgc ccttctccag
    43741 tactggaacg tcatggccgt ggtgtgtgcc atgaatgcgg gaaaccttaa acatgtggct
    43801 atggcgctgc gtgagcggta cgactcacgt atatctctcg ttattgccgg ggatgatgac
    43861 cggacatcaa aagataaccc cggttccaga gcagccaacg aagccgcatt gctggccggg
    43921 tgttacgtca cattcccgga atggccgccc ggtgcatcac cagacctttc cgactttaac
    43981 gatttgatgc tatgggaaat ggagcatgac ccggacaacc actaaaaccc cggtcacatc
    44041 cgggaagcga aaaccaccac gcaagaaaca ccccaaagtc agcccggcgc gtccgggcta
    44101 cgccacttat tcgcagcatg tacaggtgga cggcggccag atactgaaac ccggccttta
    44161 ctggcacggc gtgcgcgact caaccaacac agacacgttt atctgttccc cgctggaggt
    44221 catcgccatg accagcgaca gcaaagacga aaactacggg cgattactcc gttttctcac
    44281 gccgtcaggc cagtggcggg aatgggccgc accgatggaa atgctcgccg gagacggcgg
    44341 agaactcagg caggaactgc tacgcatggg cgttaccatt ccctacaaac aacggcaggc
    44401 actgaccgaa tacatcatgg agtcagcacc aaaacgccgg gcgctcgcgg ccacggccac
    44461 cgggtggcac tccccggccc tgtttgtcat gccgggcggg gttatcggtg atggtgatgt
    44521 ggtgtttcag tcaacagaag caggctcaca ggagtacgcc accgcaggaa cgccggaggg
    44581 ctggcgcgac aacgtggccg cattatgccg gggaaacccg gtagccatgc tggccgtgtg
    44641 tgcatcgctc gccgggccgt tgctgtggct gcttgatgtg gacagcggcg gatttcattt
    44701 catgggcgac agttccagcg ggaaaagcgt gggggcgctg gtggcctgta gcgtatgggg
    44761 agaaccccgc aactttaaac gcaactggaa cgccaccagc accgggcttg aaggactggc
    44821 gaccatgcgg aacgataccg cgctgatact ggatgaaatt ggcgaagccc cggccagaga
    44881 tattggcggg ctgatttatc agcttggcaa cggaacaggc cgccagcgtg gcaaggtgtc
    44941 cggcatggcc cgcccggtca acacatggcg caccatgatt atttccaccg gggaaatgac
    45001 cgtgggtaaa cacatggaaa gcggcggcca gcgcatcaag gccgggcagg aaatgcggtt
    45061 actggatatt ccggcacagc ggctttacgg ggcatttgac tgtcttcacg gaatggcact
    45121 accccacaac cccgacaacg acacagaacg ccggggagca ggccggaaat ttgccgaaac
    45181 gctgcgcggt aatgcggcca cccattacgg ccacgccggg ccggagtttg tgcgctggct
    45241 gatttcccgc aatgaaagtg acaccaccag caccgacagc aacggccaga cgctggcgga
    45301 tatgtacgcc atactgcgcg aacaattccc cgtaagcacc gggcaggagg cgcgcgccgc
    45361 cgcgcgtttc acactgtgcg cactggccgg ggaaatggcg ataactgccg ggattctgcc
    45421 gtggaaacag ggcgaagcac tggaggcagt gaaagcgatg tttgatgcgt gggcgggtta
    45481 tcgtggccgg ggacaatcgg aggacgtgaa aatactccgg gccgtcgcgg ggtttatcgc
    45541 ccgccatgct tcacgctttg aagggggcag cacatacagc gaaccagtaa gcaaccgcgc
    45601 cggatggtgg cgaccgggga gcaacggcga ccgggtttac tggtttttac cggaagcact
    45661 atgtgaggcg gccagcggct acgacctgaa acgcatagcg gccagccttg acgatgctgg
    45721 cgctattgtt gaccgcgaca cggacgggaa acgccgatac acaaaacgcg tttctgttaa
    45781 cggggagaaa caccgcgttt acgccatcgg ccagaatgca ttgttattcg caattgatga
    45841 ataattcaca gaaattgcgt ttttaaaaac tgaaaaagtg gtgggactgg tgggaactgt
    45901 gggaaccgta aaaataaaac atatataaat cagtaaataa taaaaccacc agtataatta
    45961 ttgttcgttt ttgagtggga actctgcggg aactggtggg aaccgcagag aacaaaaaat
    46021 aaccgccacg gttcccacaa aaagccaccg tgggaactgg tgggaaccac tgaaagccgc
    46081 gtggttctag gcggttccca cagttcccac cagtcccacc aacttgcaca atattacgca
    46141 cggcaaaaaa cgcgccataa aaacccgtaa atcccttacg tggtgcggga tgcaaggcaa
    46201 tttcagtgtg caaaacgggt taaacagcaa aaccacacag aatagcgaaa aggccagtaa
    46261 tggcgcggcc tgcgaagcat ttaccagtga attacgaata acgcttttta agggcaaaaa
    46321 tgcacttttc aacgcacaaa atcacgcgaa gccagacgcg gcgcggctta cagccgtgtt
    46381 tatatgcctg attaccgttt tctggcggca aaaaagaaca ccgaaacaca cgcgcagccc
    46441 gcgccacaca agggatacag cgaaatgagc acatcagaac ccgcagaaat tacaaaaaaa
    46501 cgccataaaa cgcacgtttt cagcgattca gaacatcaga tttatgcata agttattcag
    46561 cctgccggat gcattgtgtg agtttatccg ggcattacct gcaccggatt taccggaagc
    46621 gcaccagccc gaaacatggc cggaagacaa caccaccagc aacagccaga cgcgggcgga
    46681 tatgcgtgcc ccgtcagcgg catggctggc tgcccgtgat gcgtatatca gccacgtcat
    46741 gacctgcccg cgttgcgtta ccagcggcgt taaaaaaccg cgtcattgcg ctgagggtga
    46801 acgactacgg cagaactacg accacaccag ataaccacca ccccacggcg attaattccg
    46861 ggaacagccg ggagaatgcg ggaaaaggcg ggattaatac gggaataaac ggaaatagcc
    46921 gggatttcgt gggactaccc gccataaggc ggggtagcgg tcattattcc gcgccgttct
    46981 ccccgaccag agcacacgcg gcgcgggctg catggcattt caggccatgc cagaaatgaa
    47041 ctttttcgcc catttttacc ctgaatatgg taaaaaatgc tcacccgttg ccgggccatg
    47101 aacaaaatca gccccactat gcatggcggc cacataccac tagccccatc acagagcgcc
    47161 attgagtgcc agagagcacc acgagcgcca ttttaaaacc cgtttcaaag caccataaaa
    47221 gcggctttta catcctgttt tagcctgcac cctcgcaaag tgtgtacata agcgtgtaca
    47281 tacaacccag cacaccacca acacaaccgc acaacacaag gcacggatac agatactaaa
    47341 tattcagatt caccttcaca aatgccgccc a
//
LOCUS       DBKEKI010000016        47039 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.016, whole genome shotgun sequence.
ACCESSION   DBKEKI010000016 DBKEKI010000000
VERSION     DBKEKI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.016"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..202
                     /locus_tag="ACUQPX_000860"
     CDS             <1..202
                     /locus_tag="ACUQPX_000860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055642.1"
                     /translation="KRKAIRQDGLFALAQKKMPDATLRVLYSHICQIQQRMRFPQLAY
                     FHTCPPYQKFILKLLNNHFPAN"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACUQPX_000861"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACUQPX_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HHV9055643.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLCFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACUQPX_000862"
     CDS             complement(1614..1937)
                     /locus_tag="ACUQPX_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HHV9055644.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACUQPX_000863"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACUQPX_000863"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HHV9055645.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="patZ"
                     /locus_tag="ACUQPX_000864"
     CDS             complement(3452..6112)
                     /gene="patZ"
                     /locus_tag="ACUQPX_000864"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-lysine N-acetyltransferase PatZ"
                     /protein_id="HHV9055646.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACUQPX_000865"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACUQPX_000865"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HHV9055647.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACUQPX_000866"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACUQPX_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HHV9055648.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACUQPX_000867"
     CDS             7537..8574
                     /locus_tag="ACUQPX_000867"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HHV9055649.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMVANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACUQPX_000868"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACUQPX_000868"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HHV9055650.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACUQPX_000869"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACUQPX_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HHV9055651.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIAAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACUQPX_000870"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACUQPX_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HHV9055652.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11617
                     /locus_tag="ACUQPX_000871"
                     /pseudo
     CDS             10745..11617
                     /locus_tag="ACUQPX_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461579.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACUQPX_000872"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACUQPX_000872"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HHV9055653.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="ACUQPX_000873"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="ACUQPX_000873"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HHV9055654.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACUQPX_000874"
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACUQPX_000874"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HHV9055655.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="ACUQPX_000875"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="ACUQPX_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HHV9055656.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="ACUQPX_000876"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="ACUQPX_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HHV9055657.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="ACUQPX_000877"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="ACUQPX_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HHV9055658.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="ACUQPX_000878"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="ACUQPX_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HHV9055659.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="ACUQPX_000879"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="ACUQPX_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HHV9055660.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="ACUQPX_000880"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="ACUQPX_000880"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HHV9055661.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPSPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="ACUQPX_000881"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="ACUQPX_000881"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HHV9055662.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="ACUQPX_000882"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="ACUQPX_000882"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HHV9055663.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="ACUQPX_000883"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="ACUQPX_000883"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HHV9055664.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="ACUQPX_000884"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="ACUQPX_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HHV9055665.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACUQPX_000885"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACUQPX_000885"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HHV9055666.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVDAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /gene="acpS"
                     /locus_tag="ACUQPX_000886"
     CDS             24893..25273
                     /gene="acpS"
                     /locus_tag="ACUQPX_000886"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HHV9055667.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="ACUQPX_000887"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="ACUQPX_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HHV9055668.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFS"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACUQPX_000888"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACUQPX_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HHV9055669.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(26283..27131)
                     /locus_tag="ACUQPX_000889"
     CDS             complement(26283..27131)
                     /locus_tag="ACUQPX_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HHV9055670.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            27340..27975
                     /gene="yfhb"
                     /locus_tag="ACUQPX_000890"
     CDS             27340..27975
                     /gene="yfhb"
                     /locus_tag="ACUQPX_000890"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HHV9055671.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            28033..28536
                     /gene="tadA"
                     /locus_tag="ACUQPX_000891"
     CDS             28033..28536
                     /gene="tadA"
                     /locus_tag="ACUQPX_000891"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HHV9055672.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(28533..30089)
                     /gene="mltF"
                     /locus_tag="ACUQPX_000892"
     CDS             complement(28533..30089)
                     /gene="mltF"
                     /locus_tag="ACUQPX_000892"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HHV9055673.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSILWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            30347..34234
                     /gene="purL"
                     /locus_tag="ACUQPX_000893"
                     /gene_synonym="purI"
     CDS             30347..34234
                     /gene="purL"
                     /locus_tag="ACUQPX_000893"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HHV9055674.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVADCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            34810..36237
                     /gene="qseE"
                     /locus_tag="ACUQPX_000894"
     CDS             34810..36237
                     /gene="qseE"
                     /locus_tag="ACUQPX_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HHV9055675.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSISFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            36402..37115
                     /gene="qseG"
                     /locus_tag="ACUQPX_000895"
     CDS             36402..37115
                     /gene="qseG"
                     /locus_tag="ACUQPX_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HHV9055676.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            37105..38439
                     /gene="glrR"
                     /locus_tag="ACUQPX_000896"
     CDS             37105..38439
                     /gene="glrR"
                     /locus_tag="ACUQPX_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HHV9055677.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDQSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            38500..38838
                     /gene="glnB"
                     /locus_tag="ACUQPX_000897"
     CDS             38500..38838
                     /gene="glnB"
                     /locus_tag="ACUQPX_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HHV9055678.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(38883..40073)
                     /gene="hmpA"
                     /locus_tag="ACUQPX_000898"
     CDS             complement(38883..40073)
                     /gene="hmpA"
                     /locus_tag="ACUQPX_000898"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HHV9055679.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            40400..41653
                     /gene="glyA"
                     /locus_tag="ACUQPX_000899"
     CDS             40400..41653
                     /gene="glyA"
                     /locus_tag="ACUQPX_000899"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HHV9055680.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(41851..43044)
                     /locus_tag="ACUQPX_000900"
     CDS             complement(41851..43044)
                     /locus_tag="ACUQPX_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HHV9055681.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            43162..46437
                     /locus_tag="ACUQPX_000901"
                     /pseudo
     CDS             43162..46437
                     /locus_tag="ACUQPX_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            46534..>47039
                     /locus_tag="ACUQPX_000902"
     CDS             46534..>47039
                     /locus_tag="ACUQPX_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HHV9055682.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVIN"
BASE COUNT        11345 a        11854 c        12498 g        11342 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctctgttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagcatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtctgcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatctcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggctag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtca atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga cggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggta
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa attatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgctgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggtaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caagcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccaccg caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccattgc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggcgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaaccgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgctt cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgt cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggtta tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc gctgcgccgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttgacgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaatca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctca taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tctcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattgat gcatttttta gtgattaaca
    26221 gcgccataag gaaactctca aaacaacaca aaccgggcgg gcattatacg cgcattcttt
    26281 actcagacca gttttttcac cagcgcttca ctatgacgaa tacgttctgg tgccagttcc
    26341 agatcctgct gaatcagcgc aatgaacagc aaatccgtta acattccctg agcgtgacaa
    26401 gcagagattg aagcactgtt tgtcgcttgt tcttcggcaa tggtatacag gcaatgagaa
    26461 gcacgctgtt gcagggcatt cggagtaaag ccggtaatcg ccagcacttt tccgcccact
    26521 cgcagcatct catctgccgc caggtttaac tcgcgtcgta caccggtgta ggaaatggct
    26581 aataacagat cgtcagggga cgacgcctgt actgttgcga gtagcgcatg catatcgcgc
    26641 agtgcggcag cattgaagcc aatcttcatc agcttccagg caaagttttg cgccaccaga
    26701 cccgaagcgc caataccggt cagaattatc cgccgcgcag agcgcaacat tgttacgcat
    26761 tcatgcagtt tctcttcact attaacgttt agcgttgcgt acatagcggc agtattttct
    26821 ttaatcagtt tttcgccgac cagccgtaac ggatcatcac cgcggatttg gttatgaatg
    26881 ggcacggagg gcgattccgg ctggcttgcc agcgcttcac tcaacgccaa cttaagcgcc
    26941 ggaaaacctt tatagccgag tttttgcgcg aacttcacga cgctggactg actgactccg
    27001 gcttcgttgg ccagttgctg agagcttaaa tggcgcgccg tatcaggttg tagcagcaga
    27061 taatccgcca gttttttatc actctgggca agcccctggt aacgctgacg gatacgaagt
    27121 aagccgttca tatctttcct cgcgatgaaa tagtggcgac aacaggcaat atgagacaca
    27181 cgcgagtgaa ttttatattc cattgccgct aattgccatg aattaaaaat tccagttagc
    27241 catgatgcgg tcgattgttg ccggaaaagt gatgatctat ctgttaggct atgtagtagt
    27301 gaaaattaat caggatgttt cagtccagag gagtatggtt tggcaactca cgagcgtcgt
    27361 gtggtgtttt ttgacttaga tggaacattg catcagcagg atatgttcgg cagttttctg
    27421 cgctatttac tgcgtcgcca accgctgaat gcgttacttg tcctaccgtt gttaccgatt
    27481 atagccattg cgttattgat aaaaggtcgt gcggcacgct ggccgatgag tctgcttctg
    27541 tgggggtgca cttttggtca cagcgaagca cgtttacaga cgttgcaggc cgatttcgtg
    27601 cgctggtttc gcgacaatgt taccgccttt ccgctggttc aggagcgatt aaccacctac
    27661 ctgttaagtt ccgatgctga tatctggttg attaccggct ctccgcagcc gctggttgaa
    27721 gcggtttatt tcgatacgcc ctggctgccg cgggttaatc ttatcgccag ccaaattcag
    27781 cgtggctatg gtggttgggt attgacgatg cgttgtctgg gacatgaaaa ggtcgcacaa
    27841 ctggagcgca aaatcggcac gccgctgcgg ctgtacagtg gctatagcga cagtaatcag
    27901 gacaatccgc tgctttattt ctgtcagcat cgttggcgag taaccccgcg cggtgaactc
    27961 cagcaactgg aatagagtaa agcatagcgt ccgtgtataa tgcgccgcgc ttttataacc
    28021 ggagttttct ttttgtctga agtcgaattt agccacgaat actggatgcg tcacgcgctg
    28081 acgctggcga aacgtgcctg ggatgagcgg gaagtgccgg tcggcgcggt attagtgcat
    28141 aacaatcggg taatcggcga aggctggaac cgcccgattg gtcgccatga tcccaccgca
    28201 catgcagaaa tcatggccct gcggcagggt ggtctggtga tgcaaaatta tcgtctgatc
    28261 gacgccacgt tgtatgtcac gcttgaacca tgtgtaatgt gtgccggagc gatgatccac
    28321 agtcgcattg gtcgcgtggt ctttggtgcg cgtgacgcga aaactggcgc tgcgggatct
    28381 ttaatggatg tgctgcatca tccgggtatg aatcaccgag tggaaattac ggaaggaata
    28441 ctggcggatg agtgcgcggc gttgctcagt gacttctttc gcatgcgccg ccaggaaatt
    28501 aaagcgcaga aaaaagcgca atcctcgacg gattaatttt gtttctcttc actccctttc
    28561 ctggaaaaca gcagagaggg agaatgggtc aaatagtttg atgacgactg ggaaagaaac
    28621 gagagaaaag gaaatttctc tttgcccaac tccgtaggcg ataccgccgg ataatcctgc
    28681 gccagttgca tcgccgcttc tgtagcctgc ttctctttct cttgcagata acccaccagg
    28741 ctaatctgat acttacgaat attttcgaca taagcgtagg cttcatgccc acgagcgtag
    28801 ccgtaagtca gcttgctgta atagggtttc tggctaagta aaggcagacg ctgttttacg
    28861 tcagcccaac tgtcaggatt ccctttggtt tttgccgtca gggcgcgggc atccagcata
    28921 tgcgcatagc ccatattgta cgcagcgagg gcaaaccaga tccgttcgtt ctccggcaca
    28981 ctttccggca ctttactcat catatcctgc aaataacgca cgccaccgct gatgctctgt
    29041 tcggcatcgg tacgatccgt aatgccgagg ctttgcgcgg tatttttggt taacatcatc
    29101 atgccgcgca cacccgtcgg tgaagtggcc tgtgcatccc agtgcgattc ctgataagca
    29161 atagcggcca gcaaacgcca gtcaatttct tcggcgtatt tctcaaacag gggctttaac
    29221 tgcggcagta ccgcatcgac ggcgcgtaaa aatgtgcgcg tatcgacgta atcaaaatca
    29281 tcgccatgcc ccaggtattt ctcttcaatg cgtgccagcg taccgtcttc attcatttcg
    29341 ttgaagaagt cgagcagggc ggcggaaagg gtattatcgc catctaacgg gctaaaccaa
    29401 gtcaccggtt gttcatcggt gatatcgagc gctacggcga gctccgggtg aacgcgctga
    29461 aacaggctga tggcgacaga atcagcaatg gtgtaatcga gttttccttc gatgacatct
    29521 tccattaatt ccgcagagcc ttttttgtcg tctaccttcc agcttaattc cgggaatttt
    29581 gtttctttca gggtctggag atcgttaacc accacatgac ccggtgcaac ggtaagttgc
    29641 tccgccgtca ggttgcccag cgtacgtggg cgatactgac ccactttata aaccagttgt
    29701 tgtgacacgg aataataggt agggccaggc tgataatttt ttacccgctc actgttatag
    29761 accagtcctg ccgccagcag gtcggcgtta ccattatcaa ggtcgtcaaa cagctggctg
    29821 atattctgcc gcacggtcac tttcagtttt acgccgaggt aatcggcaaa ctgtttcgcc
    29881 agttcgtaat ccaggccaaa aggtttcccg ttgatttcgt tataagtcag gggagtatga
    29941 atggtgctca cacgcaactc tccccgcgct tgaatggcgg cgatacggtt gtcggcttta
    30001 ccaaaccaga gaatggatgg ccagagagcg accgcgagca gcagtgccag aatgccgatg
    30061 aacagataat taatctttaa ttttttcaat tagttaattc tctgtgtcgt gcgcgtccca
    30121 gcttgaaaaa acgtaataat agtgaaaggt ttactcataa atgagcggca ttttgcgtaa
    30181 acctgcgcca gatggcaact tattacagcc attggcggca cgcgttgcta attcacgatg
    30241 gtgattttat ttccacgcaa acggtttcgt cagcgcatca gattctttat aatgacgccc
    30301 gtttcccccc cttgggtaca ccgaaagctt agaagacgag agacttatga tggaaattct
    30361 gcgtggttcg cctgcactgt cggcattccg aatcaacaaa ctgctggcac gttttcaggc
    30421 tgccaggctc ccggttcaca atatttacgc cgagtatgtc cattttgctg acctcaatgc
    30481 gccgttaaac gatgatgagc acgcacaact tgaacgcctg ctgaaatatg gtccggcact
    30541 cgccagccac gccccgcaag gcaaactcct gctggtgacc ccgcgtcctg gcaccatctc
    30601 tccctggtct tcgaaagcga ccgatattgc ccataactgc gggctacaac aggtaaaccg
    30661 ccttgagcgc ggcgttgctt actatataga agccggtacg ctgaccaatg aacaatggca
    30721 gcaggttacc gctgaactgc acgaccgcat gatggaaacg gtcttttttg atttagatga
    30781 tgcagagcag ttgtttgccc accatcaacc gactccggtc acaagcgttg atttgctggg
    30841 gcagggccgt caggcgctga tcgacgctaa cctgcgtctt ggcctggctc tggcggaaga
    30901 tgaaattgac tatctgcagg atgctttcac aaagcttggt cgtaacccga acgacatcga
    30961 gctgtatatg ttcgctcagg cgaactctga gcactgccgc cacaaaattt ttaacgccga
    31021 ctggattatc gacggtgaac aacagccgaa atcgctgttc aggatgatca aaaatacttt
    31081 cgaaaccacg ccagatcacg ttctctctgc ttataaagat aacgccgccg taatggaagg
    31141 ttctgaagtg ggccgctact ttgctgacca cgaaacgggc cgctacaatt tccatcagga
    31201 accggcgcac atcctgatga aagtcgaaac tcacaaccac ccgacggcga tttctccgtg
    31261 gccgggcgcg gcgaccggtt ccggcggtga aatccgcgat gaaggtgcca ccgggcgcgg
    31321 cgcgaagccg aaagctggtt tggttggttt ctccgtatcc aacctgcgaa ttcctggctt
    31381 cgaacagccg tgggaagaag atttcggtaa gcctgagcgc attgttaccg cgctggacat
    31441 catgaccgaa ggcccgctgg gcggtgcggc gtttaacaac gaatttggtc gtccggcact
    31501 gaacggctac ttccgtactt atgaagaaaa agtgaacagc cacaatggcg aagagctgcg
    31561 cggttatcac aaaccgatca tgctggcggg cgggatcggc aacattcgcg ccgatcacgt
    31621 acaaaaaggc gagatcaacg tcggtgcgaa gctggtcgtt ctcggcggcc cggcaatgaa
    31681 catcggtctt ggcggcggcg cggcgtcttc catggcgtct ggtcagtctg atgccgacct
    31741 cgactttgct tccgtacagc gcgacaaccc ggagatggag cgtcgttgcc aggaagtgat
    31801 cgaccgttgc tggcagcttg gtgatgctaa cccaattctg tttatccacg acgtcggtgc
    31861 gggcggtctt tctaacgcga tgccggagct ggtgagcgac ggcggacgcg gcggtaaatt
    31921 tgaactgcgc gatattctaa gcgacgaacc gggcatgagc ccgctggaaa tctggtgtaa
    31981 cgaatcccag gaacgctacg tgctggcggt tgctgccgat caattaccgc tgtttgacga
    32041 actgtgtaag cgtgagcgcg caccctacgc ggtgattggt gaagcgaccg aagaactgca
    32101 tctttctctg cacgatcgtc attttgataa tcagccgatc gatctgccgc tggacgtcct
    32161 gcttggtaaa acgccgaaga tgacccgcga tgtacaaacg ctgaaagcga agggcgatgc
    32221 gctggtccgt gaagggatca ccattgcaga cgcggtgaaa cgcgtactgc atctgccgac
    32281 cgtggcggag aaaactttct tggtgaccat cggtgaccgc agcgtaaccg gcatggtggc
    32341 gcgcgatcag atggtggggc cgtggcaggt gccggtcgct gactgcgcgg tcactaccgc
    32401 cagcctcgac agctactacg gtgaagcgat ggcgattggc gagcgtgcgc cggttgcgct
    32461 gctggatttc gccgcctctg cccgtctggc ggtcggtgaa gcgttaacca atattgccgc
    32521 aacacagatt ggcgatatca aacgcattaa actttccgcc aactggatgg cggcggcagg
    32581 ccaccctggt gaagatgcgg gcctgtatga agccgttaaa gccgtgggtg aagaactttg
    32641 tccggcgctg ggcctgacga tcccggtggg taaagactct atgtcgatga aaacccgctg
    32701 gcaggaaggt aacgaagagc gcgaaatgac gtcgccgctg tcgctggtga tttctgcatt
    32761 tgcccgcgtg gaagatgtac gtcacaccat cacgccgcag ctttgtaccg aagataacgc
    32821 actgctgctg attgatttgg gcaaaggcaa taacgcgctg ggcgcaacgg cgctggcgca
    32881 ggtttatcgt cagcttggcg acaaaccggc agatgtacgc gatgttgccc aactgaaagg
    32941 cttctatgac gcgattcagg cgctggttgc acagcgtaag ctgctggcgt atcacgaccg
    33001 ctctgatggc ggcctgctgg taacgctggc ggaaatggcc tttgctggtc attgtggcat
    33061 tgacgcggat atcgcctctc tgggtgacga tcgcctggcg gcgttgttta acgaagaact
    33121 gggtgcggtg attcaggttc gtgccgctga ccgtgaagcg gtcgagtccg tactggcaca
    33181 gcatgggctt gcggattgcg tccattatgt tgggcaggcg gtttccggtg accgttttgt
    33241 gattaccgcc aacgggcaga ctgtattcag cgaaagccgc actacgttgc gtgtctggtg
    33301 ggcagaaact acctggcaga tgcagcgcct gcgtgacaac ccggagtgtg ccgatcagga
    33361 gcatcaggcg aaatctaacg acgccgatcc gggcctgaat gtaaaactgt cgttcgatat
    33421 caacgaagat gtggcagcac cgtatattgc cactggcgca cgtccgaaag ttgccgtact
    33481 gcgtgagcag ggcgtgaact cgcatgttga aatggcggca gctttccacc gtgggggctt
    33541 tgatgctatc gacgtgcata tgagcgacct gctgactgga cgtacgggac tggaggattt
    33601 tcacgccctg gtcgcgtgcg gaggtttctc ctacggtgat gtgctgggtg ccggtgaagg
    33661 ttgggcgaag tctatcctgt tcaatgaccg tgtacgcgat gagtttgcaa ccttcttcca
    33721 tcgtccgcaa acgctggccc tgggggtatg taacggttgc cagatgatgt ctaacctgcg
    33781 tgaactgatc ccggggagcg agttgtggcc acgttttgtg cgtaacacct ccgatcgctt
    33841 tgaagcgcgt ttcagcctgg ttgaagtaac ccaaagcccg tctctgctgt tgcaggggat
    33901 ggtgggctcg caaatgccga ttgctgtctc tcatggtgaa gggcgcgtgg aagtgcgtga
    33961 tgcggcgcat ctggcggcac tggaaagcaa agggctggtg gcactgcgct atgtcgataa
    34021 cttcggcaaa gtcactgaaa cctacccggc taacccgaac ggttcaccga acggtattac
    34081 ggcggttacg actgaaagtg gtcgagtcac cattatgatg ccgcacccgg aacgtgtttt
    34141 ccgtactgtc agcaactcct ggcatccgga aaattggggc gaggatggcc catggatgcg
    34201 cattttccgc aatgcgcgta agcagttggg gtaagtcatc agttcattgg ttttgcagcc
    34261 cggatgcggc atttacgccg catccggcat taagcaccaa ccactatcat aagctcccaa
    34321 ctccgggagc ttttttgtgt ctgtaaatca cgacaatggg tggtttaccg tgtcgctttc
    34381 tggcgacact taaatgattg attttattgt aatctaataa gtttatctta agttgtctct
    34441 ttttagcgac acagtggctg atttacattc aaatagcgaa gaagccataa atccctcaaa
    34501 gcaacgtata atcagtacgt taccaaacta ttttctttat tggcacagtt actgcataat
    34561 agtaagcagt ggctcattca ccgacttatg tcagcccctt cgagacgtgc tacataaaat
    34621 tcgaatgacg cacaacaagg tgcctgccgt ccaacttctg atatcagcgt agctatatca
    34681 accatcgggc gaaacgtcga gttaggcacc gccttattcc ataacaaagc cgggtaattc
    34741 ccggctttgt tgtatctgaa cttcccctcg gttagcatca ggctattcgt gtctgacgag
    34801 agtaacacct tgaaacgctg gcccgttttt ccccgctcat tacgacaact ggtaatgctg
    34861 gcatttttgc taattctact acccctgttg gtgctggcat ggcaagcctg gcaaagcctg
    34921 aatgcgctta gcgatcaggc ggcgctggtt aaccgcacta cgcttattga tgcccggcgt
    34981 agtgaagcga tgaccaacgc ggcgctggag atggagcgta gctaccgtca gtattgcgtg
    35041 ctggacgacc ctacgcttgc gaaggtttat caaagccagc gcaagcgtta cagcgaaatg
    35101 ctcgatgccc acgcaggcgt actgccagac gataaacttt accaggcatt gcgtcaggat
    35161 ttgaacaatc tggcccaact tcagtgtaac aacagtggtc ccgatgctgc cgccgccgcg
    35221 cgtcttgaag cctttgccag tgccaatacc gaaatggtgc aggccacgcg cacagtggtg
    35281 ttctctcgtg ggcagcaact tcagcgtgaa atcgccgaac gtgggcaata ttttggttgg
    35341 caatcgctgg tgctatttct ggtgagtctg gtgatggtgc tacttttcac gcggatgatt
    35401 atcgggccgg tgaaaaatat cgagcgcatg atcaaccgtc tgggggaagg gcgttctctg
    35461 ggcaatagca tttcgttctg tggaccgagc gagttacgct cggttgggca acgtattctt
    35521 tggttaagtg agcgcctgtc atggctggaa tcccaacgcc atcaattttt aagacattta
    35581 tctcatgaat taaaaacgcc actggcgagt atgcgcgagg gcactgaatt actggctgat
    35641 caggttgtcg ggccgcttac gccagagcaa aaagaggtgg tgagcattct tgatagcagc
    35701 agccgcaatt tgcaaaaact gatcgaacaa ctgcttgatt acaaccgtaa acaggcggac
    35761 agtgcggtgg aactggagaa tgttgaatta gcaccgctgg tggagacagt ggtttctgct
    35821 catagcctgc ccgcacgggc taaaatgatg cataccgacg tcgatctcaa agcaacagct
    35881 tgcctggcgg agccaatgct gctgatgagc gtactggata atctttactc caatgcggtg
    35941 cactacgggg ctgaatccgg taacatttgc cttcgcagca gtttacatgg tgcgcgggtt
    36001 tatattgatg tcatcaatac aggcacgccc attccgcaag aggaacgcgc catgatcttc
    36061 gaaccctttt ttcagggaag ccaccagcga aaaggggcgg tgaagggcag cggtctggga
    36121 ttaagcattg ccagggattg tattcgccgt atgcaagggg aactgtatct ggtcgacgag
    36181 agcgggcaag acgtttgttt ccgcattgaa ttaccgtcgt cgaaaaacac gaaataatgc
    36241 taacagccta tcctatcagg ctattttatt tgtcagttcg gccccgggta gtgctcgaaa
    36301 tcttcacgta cgatatgtac gctccggttt ctccgcgctg tccatgtcca gactgtctgc
    36361 aacaattacg cccactggga taggctttaa gtctggtgaa tatgcgacac atttttcaac
    36421 gattattgcc ccgacggctg tggctggcag ggctgccatg tctggcattg ctgggttgcg
    36481 tgcagaatca caataagcca gccattgata cgccagcaga agaaaaaatt ccggtttatc
    36541 aactggctga ctatctttct actgaatgta gcgacatctg ggcgctgcaa ggtaaatcaa
    36601 ccgaaaccaa tccgctttac tggctgcggg cgatggattg tgctgatcgt ttaatgcctg
    36661 cgcagtcgcg tcagcaagct cgccaatatg acgacggcag ctggcaaaat acctttaaac
    36721 aagggatttt gctcgccgac gccaaaatta cgccgtatga gcgccgccag ctggtcgcgc
    36781 gtattgaagc gttaagtacc gagatcccgg cacaggttcg cccactgtat caactctggc
    36841 gcgacggtca ggcgctgcaa ctgcaactgg cggaagagcg ccaacgttac agtaaactcc
    36901 agcagtccag tgacagcgag ctggatacat tgcgccagca acatcacgtt ctacagcagc
    36961 aactggaact caccacccgc aagctggaaa acctgaccga tattgaacgc cagctctcga
    37021 cccgcaaacc ggctggaaat ttctcgccgg atacgccgca cgagagtgaa aagcccgcgc
    37081 catccaccca tgaggtcact cctgatgagc cataaacctg cgcatttatt attggtcgat
    37141 gacgatccgg gattgctgaa actgcttggc ctgcgcctga ccagcgaagg ctacagtgtg
    37201 gtcacggcgg aaagtggcgc tgaaggatta cgggtactga atcgcgaaaa agtagattta
    37261 gttatcagcg acctgcggat ggatgaaatg gacggtatgc agctatttgc tgaaatccag
    37321 aaagtgcagc cgggaatgcc agtaattatt cttactgcgc atggttctat ccccgatgcc
    37381 gttgctgcaa cacagcaggg cgtttttagt ttcctcacca agcctgtcga taaagatgcg
    37441 ctatatcagg caattgacga tgcgctggag caatccgcgc cagccaccga tgaacgctgg
    37501 cgcgaggcaa ttgtcacccg cagcccgctg atgctgcgtt tgctggaaca ggcgcggctg
    37561 gtggcgcaat cagacgtcag cgttttgatt aacggtcaga gcggcaccgg gaaagagatt
    37621 ttcgcccagg ctatccacaa cgccagcccg cgcaacagca aaccatttat tgctattaac
    37681 tgtggcgcat tgcccgagca attgctggag tcggagctgt ttggtcatgc gcgtggcgcg
    37741 tttactggcg ctgtcagcaa tcgcgaaggt ttattccagg cggcggaagg cggaacgcta
    37801 tttctcgatg aaattggcga tatgcctgcg ccgttacagg tcaaactgct gcgcgtgttg
    37861 caggagcgta aagtgcgccc gctgggcagt aaccgcgata ttgatatcaa tgtgcggatt
    37921 atttctgcca ctcaccgtga tcagtcaaaa gcgatggcgc gcggggaatt ccgcgaagat
    37981 ctctattacc gcctcaacgt tgtcagcctg aaaattccgg cactggcgga gcgcacagaa
    38041 gacattccgc tactggcaaa tcacctgttg cgccaggcgg cagagcgaca taaaccgttt
    38101 gtccgcgcgt tctctaccga tgcgatgaaa cgcctgatga ccgcgagctg gccgggtaat
    38161 gtgcgccagt tggtcaacgt gattgaacag tgcgtggcgc tgacctcatc tccggtgatt
    38221 agtgatgcgc tggtggagca ggcgctggag ggtgaaaata cggcgctgcc aacctttgtt
    38281 gaggcacgta atcattttga actcaactat ttgcgtaagc tgctgcaaat caccaaaggc
    38341 aacgtcaccc acgcggcgag aatggcgggg cgcaaccgga cagaatttta taaactgctt
    38401 tcccgacacg agctggatgc aaacgatttc aaggaatgaa ttggcgttat gtgttacgtt
    38461 tagcagatca aaagacaggc gaccttttca aggaatagca tgaaaaagat tgatgcgatt
    38521 ataaaaccct tcaagctgga cgatgtccgc gaagcactgg ccgaagtcgg tattaccggc
    38581 atgacggtga ccgaagtgaa aggctttggt cgccagaaag gccataccga gctgtaccgc
    38641 ggcgcggagt atatggtgga ttttctgccg aaagtgaaaa ttgagattgt cgtaccggac
    38701 gacattgtcg atacctgtgt cgataccatt attcgcacgg cgcaaaccgg caaaatcggt
    38761 gacggtaaaa tcttcgtctt tgacgtggct cgggtcattc gcatccgtac cggtgaagag
    38821 gacgacgcgg caatttaaac cgcgtcaagg gttgccggat gtttccatcc ggcaacatca
    38881 aattacagca ccttatgcgg gccaaagcat tcgtaatgaa tgttttcctg cttcacgccc
    38941 agatccacta actgtttcgc ggtaaactgc atgaagccaa ccgggccgca gagatagaac
    39001 tgcattgtcg gatcgctgaa cgcaccttcc agtttgctca aatccatcag accttcgcta
    39061 tcaaactgac ctttagcgcg atcggcttcg ctcggctgac gataccaggt gtgcgcggta
    39121 aagcgcggca gtgactgccc cagttcttta acttcatcag caaaggcgtg aacttcgcca
    39181 ttttctgccg catggaacca gttcacttgt gctgtgtggc ctgcttttgc cagcgtgtcg
    39241 agcattgcca gcattggcgt ttgaccaaca ccggtagaga ttaacgtcac tggtgtgtca
    39301 tctgcgacag ccataaagaa atcacctgcc ggagcgacca gtttcacgac atcgccaaca
    39361 ttggcgtgat tgtgcaacca gttggatacc tgcccaccct cttcgcgttt caccgcaata
    39421 cgatagcctt tgccatccgg tttgcgagtc aaagagtact gacgaatttc ctgatgcggg
    39481 aaaccttccg gcttcagcca gacgccgaga tattgccccg gacggtattc tgccactgcg
    39541 ccaccgtcga ccggctccag ttcgaagctg gtgataagcg cgctgcgcgg tgttttagcc
    39601 acaatgcgga aatcgcgagt accttcccaa ccaccggctt tgctggcgtt ttcgttatag
    39661 atttccgcct cgcgattgat aaatacatta gccagtacac cataggcttt accccacgcg
    39721 tccagcactt cctgccccgg gctgaacatt tcgtccagcg ttgccaacag gtgttcaccg
    39781 acgatgttgt actgttccgg tttgatctgg aagctggtgt gcttctgcgc gattttttct
    39841 accgctggca gcagcgcagg caggttttca atattactgg cgtaggcggc aatagcgtta
    39901 aacagggctt cacgttgatc gccattacgc tggttactca tgttaaaaat ttctttgagt
    39961 tctgggttat gagtaaacat acggtcgtag aaatgggcgg ttaactttgg ccccgtttcc
    40021 accagtaaag ggatggtggc ttttactgta gcgatggttt gagcgtcaag catatggtct
    40081 tccttttttg catcttaatt gatgtatctc aaatgcatct tataaaaaat agccctgcaa
    40141 tgtaaatggt tctttggtgt ttttcagaaa gaatgtgatg aagtgaaaaa tttgcatcac
    40201 aaacctgaaa agaaatccgt ttccggttgc aagctcttta ttctccaaag ccttgcgtag
    40261 cctgaaggta atcgtttgcg taaattcctt tgtcaagacc tgttatcgca caatgattcg
    40321 gttatactgt tcgccgttgt ccaacaggac cgcctataaa ggccaaaaat tttattgtta
    40381 gctgagtcag gagatgcgga tgttaaagcg tgaaatgaac attgccgatt atgatgccga
    40441 actgtggcag gctatggagc aggaaaaagt acgtcaggaa gagcacatcg aactgatcgc
    40501 ctccgaaaac tacaccagcc cgcgcgtaat gcaggcgcag ggttctcagc tgaccaacaa
    40561 atatgctgaa ggttatccgg gcaaacgcta ctacggcggt tgcgagtatg ttgatatcgt
    40621 tgaacaactg gcgatcgatc gtgcgaaaga actgttcggc gctgactacg ctaacgtcca
    40681 gccgcactcc ggctcccagg ctaactttgc ggtctacacc gcgctgctgg aaccaggtga
    40741 taccgttctg ggtatgaact tggcgcatgg cggtcacctg actcacggtt ctccggttaa
    40801 cttctccggt aaactgtaca acatcgttcc ttacggtatc gatgctaccg gtcatatcga
    40861 ttacgccgat ctggaaaaac aagccaaaga acacaagccg aaaatgatta tcggtggttt
    40921 ctctgcatat tccggcgtgg ttgactgggc gaaaatgcgt gaaatcgctg acagcatcgg
    40981 tgcttacctg ttcgttgata tggcgcacgt tgcgggcctg gttgctgctg gcgtctaccc
    41041 gaacccggtt cctcatgctc acgttgttac taccaccact cacaaaaccc tggcgggtcc
    41101 gcgcggcggc ctgatcctgg cgaaaggtgg tagcgaagag ctgtacaaaa aactgaactc
    41161 tgccgttttc cctggtggtc agggcggtcc gttgatgcac gtaatcgccg gtaaagcggt
    41221 tgctctgaaa gaagcgatgg agcctgagtt caaaacttac cagcagcagg tcgcgaaaaa
    41281 cgctaaagcg atggtagaag tgttcctcga gcgcggctac aaagtggttt ccggcggcac
    41341 tgataaccac ctgttcctgg ttgatctggt tgataaaaac ctgaccggta aagaagcaga
    41401 cgccgctctg ggccgtgcta acatcaccgt caacaaaaac agcgtaccga acgatccgaa
    41461 gagcccgttt gtgacctccg gtattcgtgt aggtactccg gcgattaccc gtcgcggctt
    41521 taaagaagcc gaagcgaaag aactggctgg ctggatgtgt gacgtgctgg acagcatcaa
    41581 tgatgaagcc gttatcgagc gcatcaaagg taaagttctc gacatctgcg cacgttaccc
    41641 ggtttacgca taagcgaaac ggtgatttgc tgacaatgtg ctcgttgttc atgttggatg
    41701 cggcatgaac accttcttag gcctacaaaa tcttgcaaat tcaatatatt gcaatctccg
    41761 tgtaggcctg ataagcgtag cgcatcaggc aatttttcgt ttatgatcat caaggcttcc
    41821 tttgggaagc ctttctacgt tatcgcgcca tcaaatctgt cgtaactgcg cctcaacata
    41881 caaatagcca attcccagca cctgttgtgc gcggcttaat tgcccgaagc caatttgcgt
    41941 cgctttcacg ctcggtcctt cgtcgcggtc gaacgggtta aatcctgtct ggcgaataat
    42001 agtattaagc cagttctcac caaaggcaca actgcgaccg tacaaccaaa tctgattaat
    42061 attgaggatg ttgaggaagt tatacagact caaaccaatg gcattggcag agcgatcaac
    42121 ccagctggtg atccacggtt ctccactctg ccaggcagcg attaactgcg cggttgtcag
    42181 tttttcagga tcaagctgag tattaaccgg ttgtgatttt agccataccc gcgcctgttt
    42241 ttttaatgca cttaacgagg cgacggtttc caggcagccg taacgtccac agtcgcagac
    42301 gacgccatcc ggattaacga tggtgtgacc aatctgtccg ctaccataca aactgccgcg
    42361 ataaatttgc tcgttaatca cgaacgacga gccaatgccg taatcaacgt tgatcacgca
    42421 gaaatcccgt tcctgcgaat tattttgcca tttctccgcc agcgccagca tcacgcagtc
    42481 attatcgacc agcacccgaa tgcccagctt ctcttccagc agatacttca cctcaactgg
    42541 cgttgtccac ggcgcttgcg gcatggtttg cgatacgccg gtcaccggat caacctgccc
    42601 gtggattgcc agcgccagat tgatggtgcg gtccggccac aatttacggt ggcgatgcca
    42661 gcatttttcg atttcggaca gcagcgcctg cggcgtcggt acatcaatct gtaaatactc
    42721 aaattcgcct ttcggactta aacaagcatt agcaacctga cactcaatac tggtgggcgt
    42781 cacgttcagg cacagcgtcc agtcaccttc cggcgcaatc agccatgtac cgctactatg
    42841 cccgcgcgtc tggctttcat cgtcaatatt caccacccgt ttttcgcttt ccagttcctg
    42901 caaaatatta ctgactgccg gaatcgaaat ttgcgccagc cgggccagcg ttgatttatt
    42961 ggcgcgcttt tgccggtaca gcagttccag aatcacgcat ttgttatggt ggcgaatctg
    43021 ttgattgtta atgcaggctc tcatcttcac taaaccttgt ttagttaatt gcgtgattat
    43081 tccgcgaaaa cattgccaga ttgttcaata cactgccaca aatcttttaa ttcagtatgt
    43141 cttgttaata ttgagggcac catgactcca gtaaaagtgt ggcaagagcg cgttgagatc
    43201 ccgacctatg aaaccgggcc gcaggatata catcccatgt tcctggaaaa tcgcgtttat
    43261 cagggatcgt ccggcgcggt ttatccctac ggcgtgaccg atacgctgag cgagcagaaa
    43321 accctgaaat cctagcaggc ggtgtggctg gaaaacgact acatcaaagt gatgatcctg
    43381 ccggaactgg gcggtcgggt gcatcgcgca tgggataaag tgaaacagcg cgattttgtc
    43441 tatcacaatg aagtcattaa acctgcgctg gtggggctgc tgggaccgtg gatctccggc
    43501 gggattgagt ttaactggcc gcaacaccat cgcccgacca cctttatgcc cgttgatttc
    43561 accctcgaag cccatgaaga cggtgcacag acggtgtggg tcggcgaaac ggagccgatg
    43621 catggtttac aggtgatgac aggtttcacc ctgcgccctg accgggcggc actggaaatc
    43681 gccagccgcg tctataacgg caacgccacg ccgcgtcatt tcttgtggtg ggccaacccg
    43741 gcagtgaaag ggggtgaagg gcatcagagc gttttcccac cggatgtgac tgcggtgttt
    43801 gatcacggca aacgggccgt ctccgctttc cccatcgcca cttactacaa agtggactac
    43861 tccgccggag tggacatttc tcgctataaa aatgtgcctg ttccaacctc atatatggcc
    43921 gaaaaatcgc agtacgattt tgtcggcgcg tggtgtcacg atgaagatgg cggtttgctg
    43981 cacgttgcca accaccatat tgcgccaggt aaaaaacagt ggagttgggg acacagtgaa
    44041 tttggccagg cgtgggataa gagtctgacc gacaataacg gcccgtatat cgaactgatg
    44101 accggtattt ttgccgataa ccagcctgat tttacctggc ttgatgctta cgaagagaag
    44161 cgtttcgagc agtatttcct gccttatcat tctctgggca tggtgcaaaa tgcctcccgc
    44221 gatgcggtga taaaactcca gcgtagtgag cgggggattg agtgggggct gtatgccatc
    44281 tctccgttga acggataccg tctggcgatc cgcgaaatcg gcaaatgcaa cgcgttgctc
    44341 gatgatgccg tggccctgac accagcgacc gccatctagg gcgtgttgca cggtatcaat
    44401 cctgacagac tgaccattga actctccgat gccgacggca atattgtact gagttatcag
    44461 gaacatcagc cgcaggagtt gccgttgccg gacgtcgcca aagcgccact ggcagcacaa
    44521 gacattacca gtacagatga agcctggttt atcggtcagc atctggagca atatcatcac
    44581 gccagccgtt caccgttcga ttactacctg cgcggcgtgg cgctggaccc gctggattat
    44641 cgctgtaacc tggcgctggc gatgctggaa tataaccgcg cagatttccc gcaagcggtg
    44701 gcgtatgcca gtcaggcgct gaaacgcgca catgcgctga acaaaaaccc gcagtgtgga
    44761 caagcaagtt tgattcgcgc cagtgcttac gaacgtcagg gacaatatca acaagccgaa
    44821 gaggatttct ggcgtgcggt ctggagcggc aatagtaaag caggcggcta ttatggtctg
    44881 gcacgactgg cggcgcgtaa tgataacttc gacgcgggtc tggacttttg ccaacaaagt
    44941 cttcgtgcct gcccaaccaa tcaggaagtg ctttgcttgc ataatctgct gctggtgtta
    45001 agtggtcgtc aggacaacgc gcgtttgcag cgcgagaaac tgctgcgtga ttatccgctg
    45061 aacgccactc tgtggtggct gaactggttc gatggtcgta gcgaatcagc cctcgcgcag
    45121 tggcgcggtc tgtgtcaggg acgcgacgtt aacgctctga tgaccgccgg gcaactgatt
    45181 aactggggaa tgcccaccct ggcggcagag atgctgaacg cactggactg ccagcgcacg
    45241 ctgccgcttt acctgcaagc cagcttgctg ccgaaagccg aacgtggcga actggtcgca
    45301 aaagccattg atgtcttccc gcagtttgtc cgtttcccga atacgctgga agaagtggcg
    45361 gcgctggaga gtattgaaga gtgctggttt gctcgccatt tactggcctg cttctactac
    45421 aacaaacgta gctacaacaa agccattgcc ttatggcaac gttgcgtaga gatgtcgccg
    45481 gagtttgccg acggctggcg cgggttagcg atccatgcgt ggaataagaa acacgattat
    45541 gagctggccg cgcgttatct tgataatgct tatcagcttg cgccgcagga tgcacgtctg
    45601 cttttcgaac gggatttgct ggataagtta agtggagcca caccggagaa acgactggcg
    45661 cgtctggaaa ataatctgga aattgcgctg aaacgcgacg acatgaccgc agaactgctc
    45721 aatttgtggc atctcacggg tcaggcagac aaagcggcgg acattctcgc cacgcgtaaa
    45781 ttccacccgt gggaaggcgg ggaagggaag gtcaccagtc agtttatcct caaccagtta
    45841 ttacgcgcct ggcagcatct tgatgccaga gagccgcagc aggccagcga actgcttcat
    45901 gccgcgctgc attatccgga gaatttaagc gaaggccgtt taccggggca aactgataac
    45961 gacatctggt tctggcaggc gatatgcgcc aacgcgcagg gcgatgaaac tgaagcgatg
    46021 cgttgtttac gtctggcggc gaccggcgat cgcaccatta acatccacag ttattacaac
    46081 gatcagccgg ttgattatct cttctggcaa ggaatggcgc tgcgactgct gggtgaacag
    46141 caaaccgcac agcaactgtt tagtgaaatg aaacagtggg cgcaagagat ggcgaaaacc
    46201 agtattgagg cggatttctt tgctgtttca caacctgacc tgttatcgct gtatggcgat
    46261 ttacaacagc agcataaaga aaaatgcctg atggtggcga tgctggcgtc cgcgggactc
    46321 ggggaggttg cgcaatatga gtctgctcgc gctgaattga cggcgattaa tccggcctgg
    46381 ccgaaagcgg cattattcac cactgtgatg ccttttattt ttaactacgt tcactaaacc
    46441 ctattaactg taatatttga gcggcacgcg ctgccgctca tcacaaaata attagaataa
    46501 gccgttgtta ataaaaaccc tacaggaggc actatgccta aaaaaatgag aacaacccgt
    46561 aatctattat taatggctac gctattaggt agcgcactat ttgccagggc tgcggataaa
    46621 gaaatgacca ttggtgcaat ataccttgat acccagggat attacgctgg agtgcgtcag
    46681 ggcgttcagg atgcggcaaa agattcttca gtacaggtac agttaattga aactaacgcc
    46741 cagggtgata tttcgaaaga aagtaccttt gttgataccc tcgtggcgcg taatgtcgat
    46801 gccattattt tatcggcagt gtctgaaaat ggcagtagcc gtaccgttcg tcgcgccagt
    46861 gaagcgggta ttccggtgat ttgctacaac acctgtatta atcaaaaggg tgtcgataaa
    46921 tatgtctcgg cgtatctggt cggcgatcca ctggaatttg gtaaaaaact gggtaacgct
    46981 gccgccgatt attttattgc caataaaatt gaccagccga aaattgccgt catcaattg
//
LOCUS       DBKEKI010000017        46999 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.017, whole genome shotgun sequence.
ACCESSION   DBKEKI010000017 DBKEKI010000000
VERSION     DBKEKI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.017"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..582
                     /locus_tag="ACUQPX_000903"
     CDS             <1..582
                     /locus_tag="ACUQPX_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9055683.1"
                     /translation="SFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVL
                     VYYLSGTINSLGSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTG
                     NAGLVNVNLSLTATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDAD
                     SIARMNVYPVSTTGVPPETGRFEATATVRINFD"
     gene            608..1135
                     /locus_tag="ACUQPX_000904"
     CDS             608..1135
                     /locus_tag="ACUQPX_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9055684.1"
                     /translation="MYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            1143..1658
                     /locus_tag="ACUQPX_000905"
     CDS             1143..1658
                     /locus_tag="ACUQPX_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9055685.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            1624..2361
                     /locus_tag="ACUQPX_000906"
     CDS             1624..2361
                     /locus_tag="ACUQPX_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HHV9055686.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIANVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            2472..3482
                     /gene="pyrD"
                     /locus_tag="ACUQPX_000907"
     CDS             2472..3482
                     /gene="pyrD"
                     /locus_tag="ACUQPX_000907"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HHV9055687.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            3656..4198
                     /gene="zapC"
                     /locus_tag="ACUQPX_000908"
     CDS             3656..4198
                     /gene="zapC"
                     /locus_tag="ACUQPX_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HHV9055688.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(4195..5304)
                     /gene="ycbX"
                     /locus_tag="ACUQPX_000909"
     CDS             complement(4195..5304)
                     /gene="ycbX"
                     /locus_tag="ACUQPX_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HHV9055689.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            5548..7656
                     /gene="rlmKL"
                     /locus_tag="ACUQPX_000910"
     CDS             5548..7656
                     /gene="rlmKL"
                     /locus_tag="ACUQPX_000910"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HHV9055690.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSSDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWAVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            7668..9576
                     /locus_tag="ACUQPX_000911"
                     /pseudo
     CDS             7668..9576
                     /locus_tag="ACUQPX_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            9706..10959
                     /gene="pqiA"
                     /locus_tag="ACUQPX_000912"
     CDS             9706..10959
                     /gene="pqiA"
                     /locus_tag="ACUQPX_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HHV9055691.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGIGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            10964..12604
                     /gene="pqiB"
                     /locus_tag="ACUQPX_000913"
     CDS             10964..12604
                     /gene="pqiB"
                     /locus_tag="ACUQPX_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HHV9055692.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            12601..13164
                     /gene="pqiC"
                     /locus_tag="ACUQPX_000914"
     CDS             12601..13164
                     /gene="pqiC"
                     /locus_tag="ACUQPX_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HHV9055693.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            13420..13587
                     /gene="rmf"
                     /locus_tag="ACUQPX_000915"
     CDS             13420..13587
                     /gene="rmf"
                     /locus_tag="ACUQPX_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HHV9055694.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(13657..14175)
                     /gene="fabA"
                     /locus_tag="ACUQPX_000916"
     CDS             complement(13657..14175)
                     /gene="fabA"
                     /locus_tag="ACUQPX_000916"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HHV9055695.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(14244..16004)
                     /locus_tag="ACUQPX_000917"
     CDS             complement(14244..16004)
                     /locus_tag="ACUQPX_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHV9055696.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            16189..16641
                     /gene="matP"
                     /locus_tag="ACUQPX_000918"
     CDS             16189..16641
                     /gene="matP"
                     /locus_tag="ACUQPX_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HHV9055697.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(16717..17763)
                     /gene="ompA"
                     /locus_tag="ACUQPX_000919"
     CDS             complement(16717..17763)
                     /gene="ompA"
                     /locus_tag="ACUQPX_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HHV9055698.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(18119..18628)
                     /gene="sulA"
                     /locus_tag="ACUQPX_000920"
     CDS             complement(18119..18628)
                     /gene="sulA"
                     /locus_tag="ACUQPX_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HHV9055699.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEKEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            18847..19476
                     /gene="sxy"
                     /locus_tag="ACUQPX_000921"
     CDS             18847..19476
                     /gene="sxy"
                     /locus_tag="ACUQPX_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HHV9055700.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFVMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(19439..21601)
                     /gene="yccS"
                     /locus_tag="ACUQPX_000922"
     CDS             complement(19439..21601)
                     /gene="yccS"
                     /locus_tag="ACUQPX_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HHV9055701.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(21611..22057)
                     /locus_tag="ACUQPX_000923"
     CDS             complement(21611..22057)
                     /locus_tag="ACUQPX_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HHV9055702.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            22180..24234
                     /gene="helD"
                     /locus_tag="ACUQPX_000924"
     CDS             22180..24234
                     /gene="helD"
                     /locus_tag="ACUQPX_000924"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HHV9055703.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(24266..24724)
                     /gene="mgsA"
                     /locus_tag="ACUQPX_000925"
     CDS             complement(24266..24724)
                     /gene="mgsA"
                     /locus_tag="ACUQPX_000925"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HHV9055704.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(24820..25482)
                     /locus_tag="ACUQPX_000926"
     CDS             complement(24820..25482)
                     /locus_tag="ACUQPX_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HHV9055705.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            25655..26068
                     /locus_tag="ACUQPX_000927"
     CDS             25655..26068
                     /locus_tag="ACUQPX_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HHV9055706.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(26113..26430)
                     /gene="hspQ"
                     /locus_tag="ACUQPX_000928"
     CDS             complement(26113..26430)
                     /gene="hspQ"
                     /locus_tag="ACUQPX_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HHV9055707.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(26488..27678)
                     /gene="rlmI"
                     /locus_tag="ACUQPX_000929"
     CDS             complement(26488..27678)
                     /gene="rlmI"
                     /locus_tag="ACUQPX_000929"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HHV9055708.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            27773..28051
                     /gene="yccX"
                     /locus_tag="ACUQPX_000930"
     CDS             27773..28051
                     /gene="yccX"
                     /locus_tag="ACUQPX_000930"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HHV9055709.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(28048..28377)
                     /gene="tusE"
                     /locus_tag="ACUQPX_000931"
     CDS             complement(28048..28377)
                     /gene="tusE"
                     /locus_tag="ACUQPX_000931"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HHV9055710.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(28468..29127)
                     /gene="yccA"
                     /locus_tag="ACUQPX_000932"
     CDS             complement(28468..29127)
                     /gene="yccA"
                     /locus_tag="ACUQPX_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HHV9055711.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(29334..29421)
                     /locus_tag="ACUQPX_000933"
     tRNA            complement(29334..29421)
                     /locus_tag="ACUQPX_000933"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29385..29387),aa:Ser,seq:tga)
     gene            29847..30965
                     /gene="hyaA"
                     /locus_tag="ACUQPX_000934"
     CDS             29847..30965
                     /gene="hyaA"
                     /locus_tag="ACUQPX_000934"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HHV9055712.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            30962..32755
                     /gene="hyaB"
                     /locus_tag="ACUQPX_000935"
     CDS             30962..32755
                     /gene="hyaB"
                     /locus_tag="ACUQPX_000935"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HHV9055713.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALVIVQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            32774..33481
                     /gene="hyaC"
                     /locus_tag="ACUQPX_000936"
     CDS             32774..33481
                     /gene="hyaC"
                     /locus_tag="ACUQPX_000936"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HHV9055714.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            33478..34065
                     /gene="hyaD"
                     /locus_tag="ACUQPX_000937"
     CDS             33478..34065
                     /gene="hyaD"
                     /locus_tag="ACUQPX_000937"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HHV9055715.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            34062..34460
                     /gene="hyaE"
                     /locus_tag="ACUQPX_000938"
     CDS             34062..34460
                     /gene="hyaE"
                     /locus_tag="ACUQPX_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HHV9055716.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQVPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            34457..35314
                     /locus_tag="ACUQPX_000939"
     CDS             34457..35314
                     /locus_tag="ACUQPX_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HHV9055717.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            35448..36992
                     /gene="appC"
                     /locus_tag="ACUQPX_000940"
     CDS             35448..36992
                     /gene="appC"
                     /locus_tag="ACUQPX_000940"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HHV9055718.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            37004..38140
                     /gene="appB"
                     /locus_tag="ACUQPX_000941"
     CDS             37004..38140
                     /gene="appB"
                     /locus_tag="ACUQPX_000941"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HHV9055719.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVASNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            38152..38244
                     /gene="cbdX"
                     /locus_tag="ACUQPX_000942"
     CDS             38152..38244
                     /gene="cbdX"
                     /locus_tag="ACUQPX_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HHV9055720.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            38324..39622
                     /gene="appA"
                     /locus_tag="ACUQPX_000943"
     CDS             38324..39622
                     /gene="appA"
                     /locus_tag="ACUQPX_000943"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HHV9055721.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(39737..41903)
                     /gene="etk"
                     /locus_tag="ACUQPX_000944"
                     /pseudo
     CDS             complement(39737..41903)
                     /gene="etk"
                     /locus_tag="ACUQPX_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(41923..42369)
                     /gene="etp"
                     /locus_tag="ACUQPX_000945"
                     /gene_synonym="yccY"
     CDS             complement(41923..42369)
                     /gene="etp"
                     /locus_tag="ACUQPX_000945"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HHV9055722.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(42357..43496)
                     /locus_tag="ACUQPX_000946"
     CDS             complement(42357..43496)
                     /locus_tag="ACUQPX_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HHV9055723.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(43542..45638)
                     /locus_tag="ACUQPX_000947"
     CDS             complement(43542..45638)
                     /locus_tag="ACUQPX_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HHV9055724.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDNGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(45638..46384)
                     /locus_tag="ACUQPX_000948"
     CDS             complement(45638..46384)
                     /locus_tag="ACUQPX_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HHV9055725.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(46381..>46999)
                     /gene="gfcB"
                     /locus_tag="ACUQPX_000949"
     CDS             complement(46381..>46999)
                     /gene="gfcB"
                     /locus_tag="ACUQPX_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HHV9055726.1"
                     /translation="ALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQIQALPYSTMY
                     LRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLLEVTNPGRDP
                     LRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNVLCNVWQEEV
                     TSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
BASE COUNT        11506 a        11667 c        12423 g        11403 t
ORIGIN      
        1 agcttttccc tgaaaatatt gcagccgttc gttggttcgg tcgtcattcc tcctatggcg
       61 ctggcgcgat tatttgaatg ctacaacata cccgcaggtg attcctgcac gactactggt
      121 acaccggttt tagtgtatta cctgtctggt acgatcaatt cacttggctc atgttccgtc
      181 aatgccggag aaacaatcga ggttgatctg ggcgacgtat ttgcggctaa ctttcgtgtt
      241 gtagggcata agcctcttgg ggccagaacg gcagaacttg caattccggt caggtgtaac
      301 acgggaaacg cagggttagt taacgtcaat ctgagtctga cggcaacctc agaccccagc
      361 tatccccagg cgattaagac gtcacgtcct ggcgtgggcg tggtggtgac cgatagccag
      421 aacaacatta tttcccctgc tggtgggaca ctaccgctct ctattcctga tgatgcagac
      481 agtatcgcgc gaatgaatgt ctatccagtc agcacgacag gcgtaccacc agaaaccggg
      541 cgatttgaag ccacggcaac ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga
      601 aaaaacgata tatcagtgcg ttattttgtt ctttagcctt cttaacatcc atgtcgggat
      661 ggctgggcct gaacaagtca gtatgcatat ttatgggaat gtggtcgatc agggctgtga
      721 tgtcgccacc aaaagtgcat tacaaaatat tcatattggt gattttaata tcagtgattt
      781 tcaggccgcg aataccgtaa gcactgctgc tgatttgaat attgatatca ccggttgtgc
      841 cgctggtatt actggcgcgg acgtcctttt tagcggcgag gctgacaccc ttgcgccgac
      901 actgctcaaa ctaactgaca caggcggaag cggtggtatg gcaacgggga ttgccgtgca
      961 aattcttgat gcgcaaagtc agcaagaaat cccgctcaat caggtccagc ctcttacgcc
     1021 cttaaaagcc ggggataaca cactcaaata tcaacttcgt tataagtcca caaaggcggg
     1081 agcaacgggc ggtaatgcga cggcggttct ctattttgat ctggtttacc agtgaaggag
     1141 tgatgttgaa acggataatc tggattctgt tcttactggg attaacgggg ggctgtgagc
     1201 tatttgccca tgatggcacg gtcaacatta gcggatcgtt tcgccgtaat acatgcgtgc
     1261 tggcacagga tagcaagcaa atcaatgtgc agttaggcga tgtctcgctg acacgttttt
     1321 ttcatgggaa ttatggcccg gagaaatctt tcatcattaa tttgcaggat tgcggaacag
     1381 atgtcagcac ggttgacgtc accttttcag gaacccccga cggagtgcag agcgaaatgc
     1441 tgtccattga aagtggtacg gatgcagcca gtgggctggc aattgcgatt ctggatgatg
     1501 cgaagatatt gattccgctt aaccaggcga gtaaagacta cagcctacat agcggtaagg
     1561 tgccattaac tttttatgcg caattgcgac ctgtcaatag cgatgtgcag tcgggtaaag
     1621 tgaatgccag cgcgacattt gtacttcatt atgactaata cgtggaacag attagcgctc
     1681 ttgattttcg ccgttttatc gctgctggtg gcgggtgaat tgcaggcagg agtggtggtc
     1741 ggcggaacgc gatttatctt tccggcagac agagaatcga tatctatttt actgactaat
     1801 accagtcagg aatcctggct tattaacagc aaaatcaatc gcccaacgcg ttgggccggg
     1861 ggggaagcgt cgacagtgcc agcactatta ctggccgctc cgccacttat tctcctgaag
     1921 cccggtacta caggcacgtt gcgcttgctg agaacggaaa gcgacatctt gcctgtggat
     1981 cgcgaaacgc tatttgagtt aagcattgcc aacgtgccat ccggcaaagt tgaaaatcag
     2041 agcgtaaaag tggcgatgcg ctcggtattt aaactgttct ggcgacccga aggtttgccg
     2101 ggcgacccgc tggaagctta tcaacaatta cgctggacac ggaattcgca gggtgtacaa
     2161 ctcactaacc caacgcctta ctacattaac ctgattcagg tgagcgtaaa tggtaaagcg
     2221 ttaagtaatg cgggagtcgt gccgcctaaa agccagcgtc agacaaactg gtgtcaggta
     2281 atagcaccgt gtcatgtcgc ctggcggacg ataaatgatt acggcgggtt gagtgcaaag
     2341 aaggagcaaa atctgccctg aaacaggttc tgaaaacgtt tgcgtttttt ttgccgcagg
     2401 tcaattccct tttggtccga actcgcacat aatacgcccc cggtttgcac accgggaatc
     2461 caggagagtt catgtactac cccttcgttc gtaaagccct tttccagctc gatccagagc
     2521 gcgctcatga gtttactttt cagcaattac gccgcattac cggaacaccg tttgaagcac
     2581 tggtgcggca gaaagtgcct gcgaaacctg ttaactgcat gggcctgacg tttaaaaatc
     2641 cgcttggtct ggcagccggt cttgataaag acggggagtg cattgatgcg ttaggcgcga
     2701 tgggatttgg atcgatcgag atcggtaccg tcacgccacg tccacagcct ggtaatgaca
     2761 agccgcgtct ctttcgtctg gtagatgccg aaggtttgat caaccgtatg ggctttaata
     2821 atcttggcgt tgataacctc gtagagaacg taaaaaaggc ccattatgac ggcgtcttgg
     2881 gtattaatat cggcaaaaat aaagatacgc cagtggagca gggcaaagat gactatctga
     2941 tttgtatgga aaaaatctat gcctatgcgg gatatatcgc catcaatatt tcatcgccga
     3001 ataccccagg attacgcacg ctgcaatatg gtgaagcgct ggatgatctc ttaaccgcga
     3061 ttaaaaataa gcaaaatgat ttgcaagcga tgcaccataa atatgtgccg atcgcagtga
     3121 agatcgcgcc ggatctttct gaagacgaat tgatccaggt tgccgatagt ttagttcgcc
     3181 ataatattga tggcgttatt gcaaccaata ccactctcga tcgttctctg gttcagggaa
     3241 tgaaaaattg cgatcaaacc ggtggcttaa gtggtcgtcc gcttcagtta aaaagcacag
     3301 aaattattcg ccgcttgtca ctagaattaa acggtcgctt accgatcatc ggtgttggcg
     3361 gcatcgactc ggttatcgct gcgcgtgaaa agattgctgc gggtgcctca ctggtgcaaa
     3421 tttattctgg ttttattttt aaaggtccgc cgctgattaa agaaatcgtt acccatatct
     3481 aattatttct tcgaattcct ctttataaga taatcagggc tttattttca gccctggttg
     3541 ttttatattc atcgttgttg cttatttaga cattttgtac ttttattgtt gaggttatta
     3601 agcgaagcga caatggattg tgttgctgcg gttttatagg gtaggggaga ggcagatgcg
     3661 aattaaacca gacgataact ggcgttggta ttacgatgaa gagcacgatc gaatgatgct
     3721 cgatttagcc aatggtatgc tatttcgctc acgttttgcg cgcaaaatgt tgactccaga
     3781 tgccttttct cccgcaggct tttgcgttga tgatgccgcg ctctatttct cttttgaaga
     3841 aaagtgccgc gattttaatt tatctaaaga ccaaaaagcc gaactggtgc tgaatgcact
     3901 ggtagcaatt cgttatctca aaccgcaaat gccgaaaagc tggcattttg tctcccatgg
     3961 tgaaatgtgg gtgcccatgc cgggcgatgc ggcctgcgtt tggttgagcg atactcacga
     4021 gcaggtcaat ttgctggtcg ttgaatctgg cgaaaatgcc gcgctatgcc tgctggcaca
     4081 accctgcgtt gttattgcgg gtcgcgcgat gcagttgggc gacgccatta aaatcatgaa
     4141 cgacaggctg aaaccgcagg tgaatgttga cagcttcagc ctcgaacagg cagtctaacg
     4201 cgtcagctta agggcagtct tcggtacaca gctacagcaa agaatggtgc catcatcgcc
     4261 cattgctgat tttttcagcg gcgtgacttc gccttctaaa agctgaacac ggcaacttcc
     4321 acaaatgccc gcgcggcaag aataagggat acgaattccc tgattttcta attgctccag
     4381 caacacttgt tggttatttc cacgaaatgc ctgtccctgc cagtcaatat ctacatttgc
     4441 gtccggttgt tgcgtgatgt tggcggtatc atcagcggca gctgcgccgt aaatttttgc
     4501 cggagccgtt gccagaattt ccacctcatc gccaacgcga atcacgccgc tattacgggc
     4561 aattaaattc tgaccaaaat cgacatcgcc gttatcctgg gcagtgcgga aagattgcaa
     4621 tgtttttaat ggttcgcctg ccggatgttt ttgccctttt tctgggctga cggtggtgaa
     4681 aatacagcgg ctacaaggtt taaccacatc aaacaccaca tcaccaatgc gaatcacttt
     4741 ccagctatct tcttcccagg ctgacgcgcc ggaaaccacc agattggggc ggaattgctc
     4801 catttttaca ctggctggac aacgttgttg gagatcacgt aacgaggctt cgttagcaag
     4861 aaggtaagga tagccatcag caaatgacag aggtacagtg ttgtggcgtt tcacgcgccg
     4921 ggtcatttgt ggccccaccc agcgtaattg cacttcgcgg gagaaaaatc cacttagcca
     4981 tttgttgatc gcgtctggcg caattcgcgc ggtaaaatgt gtgccccaaa cttcggttgg
     5041 tgcgtcttgt gtggcgaaat cagcaaaacg aacatatgcg ctactgccat ctggtgcggt
     5101 gagatgcaag ccatcatgca caggtgaagg agtaaaccgt accatctggg gaaactggcg
     5161 agcggtaata aacgtaccgt caggttccgt gatcataaag atgcgatcga aggccagacc
     5221 actgacatca gccagagcat gtgtaagacc aatgccgcgc atcgatttaa caggatgaat
     5281 aaaaagccgg gttaatgtcg ccacggtgcg gtcctcaaat gaaaataagc cctcaacttt
     5341 atgacatgac gcgcttatta gctataatgc gcaacaattt tcttagcgcc tgtcccgtta
     5401 ggctatttta tttgtcagtt tggccctggg ttgtgctcga aatcctcacg tacttgtgta
     5461 cgctccggtt tctccgcgct ctccatgtcc agactgactg caacaattac gcctactgcg
     5521 gtaggctctt agagtaaaag tgacgatatg aattctctgt ttgccagtac ggcccgtggg
     5581 ctggaagagc tgttaaaaac tgaactggaa aatctggggg ccgttgaatg ccaggtggtt
     5641 cagggtgggg tccatttcaa gggcgacaca cggcttgttt accagagcct gatgtggagc
     5701 cgcttggcct cgcgtattat gttgccgctg ggcgagtgta aggtttacag cgatttagac
     5761 ctctatctcg gtgttcaggc gatcaactgg acagagatgt ttaatcctgg cgcgaccttc
     5821 gctgtccact tcagtggttt gaatgacacc atccgcaaca gtcagtacgg cgcgatgaaa
     5881 gtgaaagacg cgatcgtcga tgctttcacg cggaaaaatc tgccgcgtcc aaatgttgat
     5941 cgcgatgcgc cggatatccg cgttaacgtc tggctgcata aagaaaccgc cagtatcgct
     6001 cttgatctga gtagtgatgg tttacatctg cgtggctatc gcgatcgtgc tggtattgcg
     6061 ccgatcaaag aaaccctggc agccgctatt gtgatgcgat ccggctggca gtcaggaaca
     6121 ccgctgctcg atccgatgtg tggttccggt acgttgctga ttgaagcggc gatgctggcg
     6181 accgatcgcg caccaggctt gcaccgtggg cgttggggct ttagcggctg ggcgcagcat
     6241 gatgaagcta tctggcagga agtgaaagcg gaagcgcaaa ctcgcgcccg taaaggcctg
     6301 gctgagtata gctctcattt ttacggttcg gacagcgacg cacgggtgat tcaacgtgcg
     6361 cgcactaacg cccgtcttgc ggggattggt gaactgatca cctttgaggt gaaagatgtc
     6421 gcgcaactga ccaatccgct gccgaaaggg ccgtacggta cagtgttgag caacccgcca
     6481 tacggtgaac gtctggacag cgaaccggcg ctgattgcgc tgcatagtct gctgggccga
     6541 atcatgaaaa accagttcgg tggctggaat ctctctttgt ttagtgcctc gccggatctg
     6601 ctaagctgcc tgcagctgcg tgcagacaaa cagtacaagg cgaaaaacgg cccgctggac
     6661 tgcgtacaga aaaattacca cgttgccgaa agcacaccag acagtaaacc ggcaatggta
     6721 gcggaagatt acgccaaccg cctgcgtaag aacctcaaaa aattcgagaa gtgggcacgc
     6781 caggaaggga ttgaatgtta ccgcctgtat gacgccgatc tgccggaata taacgttgcc
     6841 gttgaccgtt atgccgactg ggcggtggtg caggagtatg cgccgccaaa aactattgat
     6901 gcgcacaaag cgcgtcagcg tctgttcgat attatcgctg caaccatttc ggtactgggg
     6961 attgcgccaa acaaactggt gctgaaaacc cgtgaacggc agaagggcaa aaaccagtac
     7021 cagaaactgg gcgagaaggg cgagtttctt gaagttaccg aatataacgc tcacttgtgg
     7081 gtgaacctga cggattatct cgatactggt ctgttcctcg atcaccgtat cgcccgccgt
     7141 atgctcggtc agatgagcaa aggtaaagat ttcctcaacc tgttttctta taccggcagc
     7201 gccaccgtgc atgcgggatt aggcggtgca cgcagcacca ccaccgtgga tatgtcgcgt
     7261 acttatctgg aatgggcaga acgcaacctg cgtctgaatg gcctgaccag gcgtgcgcat
     7321 cgcctgattc aggccgattg cctggcgtgg ctgcgtgagg caaatgaaca gttcgatctg
     7381 atctttatcg atccgccaac cttctctaac tcaaaacgaa tggaagatgc gtttgatgtt
     7441 cagcgcgatc atctggcgct gatgaaagat ttgaaacgtc tgctgcgtgc aggtgggacg
     7501 atcatgttct cgaacaacaa acgtggcttc cgtatggatc tcgacggcct ggcaaaactg
     7561 ggactgaaag cacaagaaat tacgcaaaaa acgctctccc aggatttcgc ccgtaaccgc
     7621 cagatccaca actgctggct gattaccgca gcctgaaagg aatagtaatg tcattaatca
     7681 gtatgcatgg cgcatggctg tcgttcagcg acgcgccgct tctcgataac gcagaactgc
     7741 atatcgaaga taacgaacgt gtttgtctgg tgggccgcaa cggcgcaggc aaatcgacgt
     7801 taatgaaaat cctcaaccgt gaacaagggc tggatgacgg tcgcattatt tatgagcaag
     7861 atttgattgt agctcgtctg caacaggatc cgccgcgtaa cgttgagggt agcgtttatg
     7921 atttcgttgc cgaaggcatt gaagaacaag cggaatatct gaaacgctat cacgatattt
     7981 cgcgcctggt gatgaacgac ccgagcgaga aaaatctcaa cgaactggcg aaggttcagg
     8041 aacagctgga tcaccacaac ctgtggcagc tggaaaaccg catcaacgaa gtgctggcgc
     8101 aactggggtt agatcctaac gttgcgctgt cgtcgctttc cggcggctgg ttgcgtaaag
     8161 cggcattagg acgcgcgctg gtgagcaatc cgcgcgtatt gctgctggac gaaccgacta
     8221 accatctgga tatcgaaacc atcgactggc tggaggggtt ccttaaaacc ttcaatggga
     8281 cgattatttt catctcccac gaccgttcgt ttatccgcaa tatggcgacg cgcattgttg
     8341 atctcgatcg cggcaagctg gtgacctatc cagggaatta cgaccagtac ctgcaggaaa
     8401 aagaagaagc cctgcgcgtg gaagaactac aaaatgccga gttcgatcgc aaactggcgc
     8461 aggaagaagt gtggattcgc taggggatca aagcacgccg tacccgtaat gaaggccgcg
     8521 tacgcgccct gaaagcgatg cgtcgcgaac gtggtgaacg tcgcgaagtg atgggtaccg
     8581 caaagatgca ggtggaagag gccagccgct ccggtaagat cgttttcgaa atggaagacg
     8641 tttgctacca ggttaacggt aagcaactgg tgaaagattt ttctgcccag gttctacgtg
     8701 gcgacaaaat tgccctgatt ggtccgaatg ggtgcggcaa aaccacgctg ctgaaactga
     8761 tgctcggtca gcttcaagcg gacagcgggc gtattcacgt tggcaccaaa ctggcaagtg
     8821 gcttatttcg atcagcaccg cgcggaactg gatcccgata aaacggtgat ggataacctt
     8881 gccgaaggta agcaagaggt gatggttaat ggcaagccac gccacgtatt gggctatttg
     8941 caggactttc tgttccatcc gaaacgggcg atgacgccag tacgtgcgct ttctggcggt
     9001 gagcggaacc gcttgctgct ggcgcgtttg ttcctcaaac caagcaactt attgattctt
     9061 gacgaaccga ccaacgatct tgatgtcgaa acgctggaac tgctggaaga actgatcgac
     9121 agctatcagg gcacggtatt gctggttagc cacgatcgtc agtttgtcga taacaccgtt
     9181 acagaatgct ggatcttcga aggcggcggt aaaattggtc gttatgttgg cggttatcat
     9241 gatgcccgtg gtcagcaaga acaatatgtg gcactcaaac agcctgcggt gaaaaaaagc
     9301 gaagaagccg ccgcgccaaa agcagaaact gtaaaacgca gcagtagcaa actaagctat
     9361 aaattgcagc gcgaactgga gcagctaccg caattgctcg aagatctgga ggcgaagctg
     9421 gaagccctac agacgcaagt ggcggatgct tccttcttca gtcagccgca tgagcagacg
     9481 caaaaagtgc ttgctgatat ggctgctgca gagcaggagc tggagcaagc ctttgaacgc
     9541 tgggagtatc ttgaagcgtt aaaaaatggt ggctgatcgc aaaatagtcg atttaaacag
     9601 agacagctga caacggtaaa attgcctgat gaactgggtt tatcaggcat gaaaaccaac
     9661 actttgttag tcatctgtaa aatagcgcat cattaaggag taccaatgtg cgaacatcat
     9721 catgccgcga agcacatcct gtgctcgcag tgtgacatgc tggtggcgtt accgcgcctt
     9781 gagcatggtc agaaagcggc atgtccccgg tgtggcacaa cgttaaccgt ggcgtgggat
     9841 gcccctcggc agcgtcccac cgcctatgcg ttggctgcac tgttcatgct gttgctgtcc
     9901 aacttgtttc cttttgtgaa tatgaacgtt gcaggagtta ccagtgaaat tacattactg
     9961 gaaattcccg gcgtgctttt ttctgaggac tacgccagcc tcggcacctt tttcctgttg
    10021 tttgtgcaac tggttcccgc gttttgtctg ataaccattc tgttactggt gaatcgcgcg
    10081 gaattaccgg tccgtttaaa agagcaactg gcacgggtgc tttttcaact caaaacctgg
    10141 ggaatggcgg agattttcct cgcgggtgtg ctggtcagtt tcgttaaact gatggcttac
    10201 ggcagcattg ggataggcag cagctttctc ccctggtgtt tattttgtgt cctgcaactg
    10261 cgcgcttttc agtgcgttga tcgtcgctgg ttatgggacg acatcgcccc gatgccagaa
    10321 ctgcgccagc cgctaaaacc aggcgtcacg gggatacgtc aggggctgcg ttcgtgctcc
    10381 tgttgtacgg caatccttcc tgctgatgaa cccgtgtgcc cgcgttgtag taccaaaggg
    10441 tacgttcggc gtagaaacag cctgcagtgg acactcgcgc tgcttgtaac gtccatcatg
    10501 ctgtatcttc cggctaatat tttgcccatc atggtgacgg atttattagg ctcgaagatg
    10561 ccgtcgacga ttctcgctgg ggtcattctg ttatggagcg aaggctctta tcccgtcgct
    10621 gcggtgatct ttctggccag tattatggtg ccaacgttaa agatgatcgc catcgcgtgg
    10681 ctgtgttggg atgccaaagg gcatggcaag cgcgacagtg aaagaatgca tttgatttat
    10741 gaagttgttg agtttgtagg ccgctggtcg atgattgacg ttttcgttat cgcggtgctc
    10801 tcggcgctgg tgcgtatggg aggtttaatg agtatttatc cggcaatggg tgcattaatg
    10861 tttgctttag tcgtcataat gacaatgttt tctgctatga cgtttgaccc gcgtttgtcg
    10921 tgggatcgtc aacctgaatc agagcatgag gagtcctgac agtatggaat ctaataatgg
    10981 ggaagccaaa atccagaaag tgaagaactg gtctcccgtg tggatatttc ctatcgtcac
    11041 ggcgctcatt ggggcctggg ttctttttta tcattacagc catcagggac cggaagtgac
    11101 cctgatcacc gcgaatgcgg aaggaattga aggtggcaaa accaccatta aaagccgtag
    11161 cgttgacgtc ggcgtggttg aaagcgccac actggctgat gatttgacgc acgttgaaat
    11221 caaagcgcgg ctgaattccg gtatggaaaa attgctgcat aaagacaccg tcttttgggt
    11281 ggtgaaaccg cagattggtc gcgaagggat tagcggcctg ggaacgctgc tgtctggagt
    11341 ttatatcgaa ctgcagccag gcgcgaaagg cagcaaaatg gataaatacg atttgctgga
    11401 ctcaccaccg ttggccccgc ctgatgcgaa aggtatccgt gtgattctcg atagcaaaaa
    11461 agccgggcag ctctcgccag gagatccggt gctgttccgt ggctatcggg taggttcggt
    11521 tgaaaccagc accttcgata cgcaaaaacg caatatcagt tatcaactgt tcatcaatgc
    11581 accttatgac cgactggtga ccagcaatgt tcgcttctgg aaagatagtg gcattgcggt
    11641 tgatctgacg tcagcgggga tgcgtgtgga gatgggctca ttgacaacgc tgcttagtgg
    11701 cggtgttagc tttgatgtgc cggaaggtct ggatttaggg cagccagtgg caccgaaaac
    11761 agctttcgtt ttgtatgatg atcagaagag cattcaggat tcgttgtaca ccgatcacat
    11821 tgattatctg atgttcttta aagattcggt acgcggtctg caaccgggag ctccggtaga
    11881 attccggggt attcgcctgg gtaccgtaag caaagtgcca ttctttgcgc cgaatatgcg
    11941 tcagacattt aacgatgatt accgtattcc ggtactgatt cgtatcgagc cagagcggct
    12001 gaaaatgcag cttggcgaaa atgcggatgt tgttgagcac cttggcgaat tgttgaaacg
    12061 tggtttacgc ggatcgctga aaaccggaaa cctggtcact ggtgcactgt atgttgatct
    12121 cgatttctat ccaaatacgc ctgcaataac cggtattcgt gaatttaatg gttatcagat
    12181 tatcccgact gttagcggcg gcctggcgca aatccagcaa cgactgatgg aagcgttgga
    12241 taagatcaac aaactgccat tgaatccgat gattgaacag gcaaccagta cgctttctga
    12301 aagtcagcgc acaatgaaaa acctgcaaac gacgctggat agcatgaaca agatcctcgc
    12361 cagccagtcg atgcagcagt tgccgacgga tatgcagtca acgttgcgtg aattgaatcg
    12421 cagcatgcag ggcttccagc ctggctccgc agcctacaac aagatggtgg cggatatgca
    12481 gcgccttgat caggtgttgc gagaactgca accggtgctg aaaacgctca atgagaagag
    12541 taacgcgctg gtatttgaag cgaaggacaa aaaagatcca gagccgaaga gggcgaaaca
    12601 atgaaaaagt ggctagtgac gattgcagca ctgtggctgg ccggatgcag ctccggcgaa
    12661 attaataaaa actattacca gttacctgtg gtgcagagcg gtacacaaag taccgccagc
    12721 cagggcaatc gtctgttatg ggtagagcag gtcactgttc ctgactatct ggcggggaat
    12781 ggtgtggttt atcaaaccag tgatgtgaag tatgtgattg ccaacaacaa cttgtgggcc
    12841 agcccgttgg atcaacagtt gcgcaacacc ctggttgcca acctgagtac gcaactgccc
    12901 ggctgggtgg ttgcctccca gcctctggga agcgcccagg acacgctcaa tgttaccgta
    12961 acggagttta acggtcgcta tgatggcaag gtcattgtca gtggtgagtg gctgttgaac
    13021 caccagggac aactgatcaa acgtccgttc cgtctggaag gagtgcaaac tcaggatggt
    13081 tacgatgaga tggttaaagt gctggccggt gtctggagtc aggaagccgc ttctattgca
    13141 caagagataa agcgtctacc ttaattataa agatttgtaa atataaccgt ctccggtatg
    13201 ttgcctgagg cggttttttt gtttctaacg tgcggaaaaa tttgttcctc ttcacatttt
    13261 ttgtacaacc gacatgcccg tgtagctcac aaatatgaca gtggcgtgaa ttttgcgcat
    13321 tgacggcagt tatgattcgc ggtattgctt aactgtgatt gcacatttag taatcactgt
    13381 tttcttttcc accagaaacc agtatgaggg aaacgaggca tgaagagaca aaaacgagat
    13441 cgcctggaac gggcacatca acgtggttat caggccggca tcgccggacg ctcaaaagaa
    13501 atgtgtccct atcagacgct gaatcaaagg tcacaatggc tgggaggctg gcgagaagcc
    13561 atggcggaca gggtagtaat ggcctgattc tgtctcttta aaaagaaacc tccgcattgc
    13621 ggaggtttcg ccttttgata ctctgtctga tgataatcag aaggcagacg tatcctggaa
    13681 cagaccgact ttcaggtcgc tggcggtata gatcagacga caatcaacca gcacttcgcc
    13741 atctgccaga cccataatca gacgacgatt aacaatgcgt ttaaagtgaa tacggtaggt
    13801 cacttttttc gctgtcggca gtacctgacc agtgaatttc acttcgccaa cgcccagcgc
    13861 gcggccttta ccttcgccgc ccagccagcc gaggtagaac cctaccagct gccacattgc
    13921 gtccaggccc aggcatcccg gcataaccgg atcgccaatg aagtggcatc cgaagaacca
    13981 cagatccgga ttgatatcca gttctgcttc aacataccct ttgtcgaagt taccacccgt
    14041 ttcggtcatt ttgaccacac ggtccatcat cagcatgttc ggtgctggca attgcgggcc
    14101 tttagcgcca aacagttcac cgtgaccaga ggcaagaagg tcttcttttg tataggattc
    14161 gcgtttatct accatgttct ctgtaagcct tattttattg aagcacgcag gatagctaac
    14221 acgtgtacgc tgaacaagtc cgatcagttc ggaataaacc agttcagcca acgtaatggc
    14281 catggaaaac ggtgacgtcc ttcctgttgc gatgcttgcg cgatacgttc ctggatggtt
    14341 tgcatcagcg tcgtttggcc ttcgccatcc cacaccagat ttaataataa cggcagtgcg
    14401 tcagtcacat cgtctactgc ccagatggtg aatttgcctt cttctaccgc tttcaccagt
    14461 tcactgtgaa gacttaaatg gcgaacgtta gcagtgggga taataacacc ttgtttcccc
    14521 gttaactcac gttgctggca aatagcaaag aagccttcga ttttctcatt taaaccaccg
    14581 accggctgga cgcggccgaa ctgatcgact gaacctgtga tagcgatact ctgattcact
    14641 ggcacatcag cgagggcgct tatcagggcg cagagttcag ccatcgaggc actatcgcca
    14701 tccacttcac tgtatgactg ctcaaatgtc agcgatgctg agaaggggat ctgttgctca
    14761 agctgtagtt ccgacatcag gaacgcctgc atgatcatca tccctttcgc atggatattg
    14821 ccgccaagct ccgctttgcg ttcgatgtcg gtgaattcac catcgccaat atgcacaacg
    14881 cagctaatgc gagaaggttc gccaaaagcg cgtggatgac ccggaaattc aatgaccgaa
    14941 agggcgttaa tttgcccgat gcgttcgcct tcggtttcaa tcaggatttg ctcctgaagg
    15001 atctcatcct gcatacgttc agcgaggaaa ccttcgcgcc attcacgctg ctgcagcatt
    15061 aagtttagct gctcgccgga gaaggtgtcg ccatcacaca gggaggcgac ctctttacac
    15121 tggcggagga tccactgcgg gctaagcgga agtgtttctt gttcaccggt gtagcgtgct
    15181 gcttcgcgga taagtaccgg ccaagcatcc gctcccgggg caggtaagtg attatgtctg
    15241 gcggtaaatg tcacccagcg acaccactgg cttactgact ccgcatcgac aatctgcaga
    15301 gtatcttcaa attcgctata aatagcctgc tctgaaagct ctggctccat ctcctggaaa
    15361 tcagccaatg attcgcgttc gcctaccaga atgactttca gcttcaatgg catcgaaggc
    15421 acagagacgg ggagagggcg cgactcatca aacgcaaccc agtcaaaacg ctcgcggtta
    15481 acgatatttt tcagccgcat ccacagcaga ggttgcgcca gcagtgtacg caaagagatg
    15541 atgagaatac cgccatttgc ctgatgcacc agaccaggct gcagggtaat gtcgccatta
    15601 aactggcgca ggcagccaaa gagttgctcc gcttctaccc agtcggcagc gacaacttgc
    15661 gttaaagtcg caaaattatc atctgcactc actgcgtgac gtaagcggat ggagtggccg
    15721 gaaacctcat agtgatcgcc caccagttgg cctgcatcgc tttgtagcgt acgcgcggca
    15781 tcggcaataa gattcagata ctcagactct tccggggcct tagccagcat aaaagaggag
    15841 gatgctcgcg tatgcagcaa ctgctccagc gcaaattgca gtcgcggttg agtatcactg
    15901 aataaaggat cgttttcgtc aatcaaatgt ggctgagcaa atatttcctg atagctatcg
    15961 gtatcaggaa ccaggtcacg ccatgcaagt ttcgtaatgg tcaaagttga tgttttttag
    16021 tctgttgtca aagccgcgat tataccgtaa ccggcactac agcacacgta gaaagcaccg
    16081 acaatactct tggctgggcg ttaaagctca caggatggag attcttttct tcactggcct
    16141 aaaaagctga tattctgtaa agagttacac ggtaacattg agatcgctat gaaatatcaa
    16201 caacttgaaa atcttgaaag cggttggaaa tggaagtatc tggtgaagaa gcatcgcgaa
    16261 ggggagttaa ttacccgtta catagaagcc agtgccgccc aggaagccgt cgatgtgttg
    16321 ctctcgctcg aaaatgaacc cgtgctggta aatggctgga ttgacaagca tatgaatccg
    16381 gaactggtca atcgcatgaa gcagaccatt cgggccagac ggaagcgcca ttttaatgcg
    16441 gaacaccagc atacgcgcaa aaaatcgatc gatctggaat ttattgtctg gcaacgtctg
    16501 gctggtcttg cacagcgtcg cggtaaaacg ctttctgaaa cgattgttca gctgattgaa
    16561 gatgcggaaa acaaagagaa atacgcgaat aaaatgtctt ctctgaagca ggatctgcag
    16621 gcattgctgg gtaaggaata actgacgaaa gtcagttcaa tttactaaag gcaaaaaaaa
    16681 ccccgcagca gcggggtttt tctaccagac gataacttaa gcctgcggct gagttacaac
    16741 gtctttgata cctttaactt cgatctctac gcgacgatcc ggagccaggc agtcgatcag
    16801 tgcagcacgc tgtttcacgt tgtcacaggt gttgccagta accgggttgg attcgcccat
    16861 accacgtgcg gagatcttgt ctgccgggat acctttggag atcaggtaat caacaacaga
    16921 ctgagcacgg cgctcggaca gaccctggtt gtaagcgtca gaaccgatgc ggtcagtgta
    16981 acccagaaca actacggaac cgtctttcgg atccaggttg ctcagctggc tgtacagctg
    17041 atccagagca gcctgacctt ccggtttcag ggttgctttg ttgaagttga acagaacgtc
    17101 agacttcaga gtgaagtgct tggtctgtac ttccggtgcc ggagctggag caactaccgg
    17161 agctgcttcg ccctgaccga aacggtagga aacacccagg ctcagcagac cgttgtccgg
    17221 acgagtaccg atggtgtttg cgtcaccgat gttgttggtc cactggtatt ccagacgggt
    17281 agcgatttca ggagtgatcg cgtactcaac accacctgcg aaaaccggag aaacgccagt
    17341 gtcgtggtct ttaaaggatg cgccaccagg tacgttagcc ttggtgtctg cacgccatac
    17401 cataccaccc agacgagtgt agatgtccag atcgtcagtg attgggtaac ccagtttagc
    17461 ggtcagctga acgccctgag ctttgtatgc gccgttgatg ttgtcgcctt tgtacggcat
    17521 acgacctaac cagtcgtaac ccatttcaaa accaacatac gggttaacct ggtaaccacc
    17581 aaaagcacct gcacccagtt ggttttcgtg ggtcggacca ttgttaggaa taaaaccagt
    17641 gtcatggtac tgggaccagc ccagtttagc accagtgtac caggtgttat ctttcggagc
    17701 ggcctgcgct acggtagcga aaccagccag tgccactgca atcgcgatag ctgtcttttt
    17761 cattttttgc gcctcgttat catccaaaat acgccatgaa tatctccaac gagataacac
    17821 ggttaaatcc ttcaccgggg gatctgctca atattaactc taccgatatc ttcggcttat
    17881 gccgagcacc cctggcgatg taaagtctac aacgtagttg aaaacttaca agtgtgaact
    17941 ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt ttaatctttt tgtcagcgac
    18001 aatttacaga aaaaaaacgc ggaaaccgct tcagacaagc cttcgcaagg aaaattagtc
    18061 acgactgaaa gcattggctg ggcgacaaaa aaagttccag gattaatcct aaatttactt
    18121 aatgatacaa attagagtga atttttagcc cggaaagttg tctcgttgtg tgagaggatg
    18181 cgcttaccgg gcgcataata aaccccatag cgttaccttc atttgccgca tcaacaagtt
    18241 cagcatgctc tttttcagtc aaatcatctg tcaaccaacc gatcaccaca ctgtaattgc
    18301 ccgtgcgtaa agcgcgaacc attgactcca cagtgtggca aggggagagc tggctaatct
    18361 gcattacttt cgttaagggt agcccagatg cctgaaccca ttcccgactc agtttttgtt
    18421 gcggtgttaa ccagagttgc cagcgcgatt gctgaccgag ttgctgtaac aatggcaaca
    18481 gtagaagttg cgtcatcatg ggctgatctt cgcgatagac aacttcactg ataagcccgg
    18541 ctgtagtgtt ttccgtagag acacgcgcaa ttttacttgc tgcggatgag aacgacgaag
    18601 aacgatgtgc atagcctgaa gtgtacataa tcaatccagc ccctgtgagt tactgtatgg
    18661 atgtgcagta catccagtga caacaaagat caaccctatt ttcggaaaga gcctcgcaaa
    18721 ttttgtcgtt ggtgacggga aaacataaat taatcttgcc ccttaagaat aagttgccta
    18781 ttttcgtagt taacggatcc gttaatgtga atcattcttt tatgttatga ttttaaaagg
    18841 aattttatga aaagcctctc ctataagcgg atctataaat cacaagaata cctggcaacg
    18901 ttgggcacaa ttgaataccg atcattgttt ggcagttaca gcctgaccgt tgacgacacg
    18961 gtgtttgtga tggtttctga tggtgagttg tatcttcggg cttgtgagca aagtgcacag
    19021 tactgtgtaa aacatccgcc tgtctggctg acatataaaa agtgtggccg atccgttacc
    19081 ctcaattact atcgggttga tgaaagtcta tggcgaaatc aactgaagct ggtgcgtctg
    19141 tcgaaatatt ctctcgatgc agcgctgaaa gagaaaagca cgcgcaatac ccgggaaaga
    19201 ctgaaagatt tgcccaatat gtcttttcat ctggaagcga ttctcgggga ggtggggatt
    19261 aaggatgtac gggcgttacg tatacttggg gcaaaaatgt gttggttgcg actgcggcag
    19321 caaaacagtc tggtgacaga aaagattctg tttatgcttg aaggtgccat tatcggcatt
    19381 catgaagctg cgctcccggt ggcacgccgc caggagcttg cagaatgggc tgactctctt
    19441 acgccgaaac aggagtttcc tgcggagctt gagtaatctg gcgttgcaga cgaccaatct
    19501 ctggcagtaa tgcaatcaat aacccaactt gttgtacgac cagaggttct ttgctatctg
    19561 cccgtggttc aagttgctgc atccgctgtt tcaggccagc taatgcctga ttgacgcgtt
    19621 cttcatcagc aggttgatga tgtaacgcgt catcaacata gcaaactgcg tcatcaagaa
    19681 acgccagaat ttcaggatta gttaactgct cccggtgagc accgagggct gagatatagc
    19741 tggtaaacgt atggttaagg cacagcaagc gaaacgcggc ttcgcgaatt tgcggggtaa
    19801 cgttcggttc gctggacata tttgatacca ccgacgccag ttcagcatca cggttgtgtg
    19861 catcgcggcg ggcaatacga tacgccagac ggttatcacg cccctgatgg tattgctcca
    19921 gtatggcatc gagatagcga cagttggcct ctgtggcgcg ttcgagcatt cgcggcagat
    19981 tgcgaaactt ccagtcaggc cagatgtagc tcactgccgc ccacgcaatg gcacaaccaa
    20041 tcagcgtatc gattacgcga ggtaacgcta cttcaaaacc ttcacccagt aagttaaaac
    20101 acagtagcac caaaagtgtg atgaacatcg ttgcatgagc gtattgcacg ttacggaagg
    20161 caaaaaagag cacgccggta ataaccagca gcaccagctg cccttccagt gatggcacaa
    20221 accacagcac aggaatgcca atcgcgatac ctaccagcgt accaataatc cttaacttca
    20281 ggcggtggcg cgtggcgtta tagtttggct ggcagacgaa caaacttgtc agcaagatcc
    20341 aatacccgtg atgcattccg gttatttgaa tgatggcgta gccgaagcac aacaccagcg
    20401 acattcttac cgcatgacgg aagagggcgg attcaggcgt gaagtgacgg ctaagacgca
    20461 gccagatatc actcaatccg tgcgggctgt catcagcgag ctcattttcg tcattattac
    20521 ggggtagtgc ctgggcctgt tctgattcaa ttgttgccag ttgggcatca atggcgcgta
    20581 aattgttcag caaaaatccc agtgttttga gtaaatcggc gggtgcgccg ttatcgcgca
    20641 tccgctccag cgcagcatca atatgcgtaa aagcgcgctc aaaatgcgga tcatgttgat
    20701 aagactgacg caacaaaata cagcgtgaca gttgctggca cgcctggccc tgcatcgaca
    20761 tcagccgctg aaaacggaac agcacgtcgc tgtggcgaaa atgttcacgc aatgtttgat
    20821 actgaatatg agaagagctg gcacgctcgt gaatatcctg tgcgacaaag taataatgca
    20881 gcgtgcgacg cgttccccgt tgaccacgat cgccacgtaa gcgggtcagc agcgagagtt
    20941 tcgtctgatt caatgtcgcc atcagctgac cgttggcgag agccaaatcg tacagcggtg
    21001 cctggctttc atcttcaata tcaggatcaa acatgcgcga cttgagctca agataacgcg
    21061 ccagttgttc atagcaacgc gccaggttgt cctgcagcgg gcggaccggg aacagcagat
    21121 gaccaataag tgtcaggacg ttgtaccaga cggcaccggc cagcagatac atcggctgct
    21181 gataccagtg ctcatacagt gatgttccca acatagtgta aatggcgatc agcaatgcac
    21241 cgaaggcaat tgttgcatag cgttgaccca gaccgccgag caaaatgaag ccgctggtag
    21301 agagcgttaa gccaatcgca aatagccagg gccagggaaa cagcaattct actgaggccg
    21361 aggcgataaa aaagcagaac agcgtaatga tgaggttacg caaacgtccc gccagtcggt
    21421 catcgagatc ggtcagcgcc gctgccacca tccccagcgt tagcggaatc gtcagtttta
    21481 catcacccag ccaccacgga aacgctgtgg ttccacaaag cgcaataaaa atacgcgcgt
    21541 aatacagcca ggcgctgttc caggtatagc gtttgagcaa aggacttagc ataaaggcca
    21601 tattagtgga ttattcaaaa cgacgacgtg cattggcttc acgcgcagct tgcgctgttt
    21661 ctaccgatac cacgcgacga ccaaccggcc atagtgcaat agcggcaatt ttaaagttcg
    21721 caatgccgac aggaatgcca atgattgaaa tacattgtgc gatgcccgtt gcaatgtgca
    21781 tcaggcataa ccaccagcca aagaaaatca gccagaaaat attcaatacc gtaccgccag
    21841 tattcagcaa cacatttttg ccagccgggt tcagttcatc gacatgaata gcttcattgc
    21901 cataaggcac cagagacagt ttagtgatct cccagcagga tcgtgtcagc ggtaaggtaa
    21961 aaatcagcac aatactgacc agagtcgcca acagccagcc cagagtggtg gcaaatccgc
    22021 caagcacaaa gttcagaatg ttcaaaacgg tacgcataaa acctcgcttt actgtggttt
    22081 tcagtaatgg gcggcaattg taacgttttt ttgggctgga gcacgttttc tctgacggtt
    22141 acactgataa gaaataattt cgtgtggatc tacagagtca tggaactgaa agcgacaacg
    22201 cttggaaaac gtctggcaca gcacccttac gatcgggcgg tgatcctcaa tgccgggatt
    22261 aaagtctccg gcgatcgcca cgaatacctt attcctttca atcaattact ggcgattcac
    22321 tgtaagcgcg gtctggtatg gggcgagctg gaatttgtac tgccggacga aaaagtggtg
    22381 cgtctgcacg gcacagaatg gggcgagacg cagcgttttt accatcatct tgatgctcac
    22441 tggcggcggt ggagtggcga gatgagcgaa attgcgtctg gtgttttacg ccagcaactg
    22501 gatttgattg ccacgcgcac tggagaaaat aaatggctga cgcgtgagca aacctctggc
    22561 gttcagcaac aaatccgcca ggctttgtcg gcgttgccgt tgccggttaa ccgactggaa
    22621 gaattcgata actgccgtga ggcgtggcgt aaatgtcagg cttggttgaa agatattgaa
    22681 agtgctcggt tgcagcataa ccaggcgtat accgaagcca tgcttaccga gtatgcggat
    22741 tttttccgcc aggtcgagtc ttcaccgctg aatccggcgc aggcccgggc agtcgttaat
    22801 ggcgagcatt ctctgttagt gctggcaggt gcaggaagcg gaaaaacgtc ggtgctggtg
    22861 gcccgtgcag gctggttgct ggcgcgtggt gaagcgtccc ctgagcaaat tttattgctg
    22921 gcgtttggtc gcaaagccgc tgaagagatg gacgagcgga ttcgcgaacg gctacatacc
    22981 gaagacatta ccgcacgcac gtttcatgcg ctggcgctgc atattattca gcagggcagc
    23041 aaaaaagttc cgatagtcag caaactggaa aatgataccg ctgcccgtca tgaactcttt
    23101 attgctgagt ggcgcaagca atgcagcgaa aagaaagcgc aggcgaaggg ctggcggcaa
    23161 tggctgacgg aagaaatgca gtggtcagtg ccagaaggta acttctggga tgatgaaaaa
    23221 ttacagcgtc gccttgcctc tcgcctcgat cgctgggtaa gtctgatgcg gatgcacggt
    23281 ggtgcacagg cagaaatgat tgccagtgca cccgaagaga ttcgcgatct gttcagtaaa
    23341 cgtatcaagt tgatggcccc gttattaaaa gcctggaaag gtgcgctgaa ggcagaaaac
    23401 gctgtcgatt tttcgggcct tattcatcag gcgattgtga ttctggagaa aggccgcttt
    23461 atcagcccgt ggaagcatat tctggttgat gaatttcagg atatctcgcc gcagcgcgca
    23521 gcgttgttag cggcattacg caagcaaaac agtcagacga cgttgttcgc cgttggtgat
    23581 gactggcagg cgatttaccg attcagcggt gcgcaaatgt cgctcaccac cgctttccat
    23641 gaaaactttg gtgaaggcga tcgctgtgat ttagacacga cttaccgttt taacagtcgt
    23701 atcggtgagg tggcaaaccg gtttattcag cagaacccag gccagctgaa aaagccgcta
    23761 aacagcttaa ccaatggaga caaaaaagcc gtcacgttat tggatgagag tcaacttgac
    23821 gctttgctgg ataagctctc tggttatgcc aaaccggaag agcgcattct gatcctggcg
    23881 cgttaccatc acatgaggcc tgccagcctg gaaaaagcgg caacacgctg gccgaagttg
    23941 caaatcgact ttatgaccat tcatgccagc aaagggcaac aggcggatta cgtcatcatc
    24001 gttggcttgc aggaggggag tgatggtttt ccggctgcgg cgcgggagtc gattatggaa
    24061 gaggcgctac tgccaccggt tgaggatttc ccggacgctg aagaacggcg tttaatgtac
    24121 gtggcgctga cccgggcacg ccatcgggta tgggcactgt ttaacaaaga gaatccctct
    24181 ccctttgtgg aaatactgaa aaatctggat gtgccggtgg cgagaaaacc gtaagaaaca
    24241 ggtggcgttt gccacctgtg caatattact tcagacggtc agcgagataa cgctgataat
    24301 cggggataag aatatcgacc gcgtcgttga aatgcggcga ctggataatg aagtctgccg
    24361 ttgccacgtt ggtggcgacc ggaatgttcc ataccgtcgc cagacgcagc aaggctttca
    24421 cgtcaggatc gtgcggcacg gcatttagtg gatcccagaa gaaaatcaat acatcaattt
    24481 tcccttctga gatcaatgcg ccaacctgct ggtcaccccc cattgggcca ctcaacatcg
    24541 cgttgacgtt catgccggtc gcgcgggaaa tcaagttacc ggtagtgcct gttgcataca
    24601 gtacgtgttg ttccagtaac ggttgatgcc gttccaccca gctcatcagc atttgtttgc
    24661 agtgatcgtg tgccaccagc gcaatatgtt tccgcgcagg taaagtgcga gtcgtcagtt
    24721 ccataatgta catccgtagt taactttcct acagattact gtaagcactt atcgctgcaa
    24781 gataaagacc gaaaaagcct gcgcacaggc acaaaaatct caggaagatg gttgtttttc
    24841 cgcccattgc aggaaagtat ttcgcgtttg tgggtcagcc agtttaaacc aatacttcag
    24901 ccgttgttct gtgagcacct gagactgcgg tggaatagca tccagctcgg aaacacccga
    24961 aagcagcgta ctgtcatctg ccatcatcgt ggcaaattgc ggtagtgaag cgcgtttcgc
    25021 ggatttgtta tagcgttcaa cctcgacctc ataatcaatc gtttttgccg ttgaggtaat
    25081 ggcgagaata tccagcttta ccggaatcgg cgtcgcatcg ccatccaaca attcaaggcg
    25141 cggcgcggca tcaaaatggt tagcttcccg ctcattctcc aggcgaggca ggcgaaaatt
    25201 gacctggttg atgagctggg tattaaaact gaccaccagc ggtggggaga tatacagtcg
    25261 ttcttcacta ttggaaagat gaatcgtctt ctcaacgcga aacactaact gatgcggtcc
    25321 attgtccagt tcaatgctgt ccgcaccgcg cagcagagag ctggaaacct tcttgccgtc
    25381 gagtaccaga agatcgacat cggttgaaag ccgcagcgtg gtggcaaaaa cggataccgg
    25441 caaacataat gcaatcaagg tggtcacgat gccggttttc ataggtagct cctgtcaaaa
    25501 gaccgcccga ataataatgt gtcaaaattt ttctttaatc atacttacta acatatagac
    25561 atatttcccg cttttgctct cattcattcg tattagctgc atggttggca tgtcggcttt
    25621 gtcgtatact ttgttaaaca gtctggagga aataatgaaa gaaaccgata ttgcaggcat
    25681 tttaacgtcc actcacacca ttgcgctggt gggggcaagc gacaaacccg atcgccccag
    25741 ctatcgcgtg atgaaatatc tactcgacca ggggtatcac gtcattccgg tttcgccaaa
    25801 agttgccggc aaaacgctgc tgggccagca ggggtatggc acgctggcag acgttccgga
    25861 aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg gcgtggggcg tggcacagga
    25921 agctattgcc attggcgcga aaaccctctg gatgcaactg ggggtgatta atgagcaagc
    25981 ggcagtactg gcacgggatg ccgggttaaa tgtggtgatg gatcgctgcc cggctattga
    26041 gatccctcgc ctgggtctgg ccaaataaaa aatccccgga aggcaaaaac cttccgggga
    26101 tttgttcagg gattagttac gcagacgcgg ggcctggagt tgtttgcgga tggtctgcgc
    26161 cagctcatcc atggatggct gttcaggatg ttcatcctgc aattcgctac ttagctgggc
    26221 ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc tccatcacca cgtgatacca
    26281 cggagcggcg cgaagctcgt cattcaccgc cagctcatca ggcgacggtt cagagagcga
    26341 ataaaccggg tcgatatcca cgaccactcc gagataacct aacagggaat ggcggacctg
    26401 ctggccgata ccgaatttgc tggcaatcat agtcacctcc cgggaaatct ttacctttga
    26461 tataagggta aaattccaca tttcaagtta catgacgcga caggcaaacc ctttcagata
    26521 tagcccttcc gggtaggtag cgatcaccgg atgatcggct gcctgacgga actgctctat
    26581 aaattgtaca tcacgaccgg catcaattgc ggcatccgcg atgattttct gaaataaatc
    26641 gctggtcatc agaccggaac aggagaaagt caggagaata ccgccttcat tcagcaactg
    26701 aatcgccagc atgttgatgt ctttataacc acgacacgcg cccatcaact ggcttttatt
    26761 ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat ttttcaccgc gatcgcgata
    26821 agtacgcagc aatttaaaga catcatcacg gacaaactca gccttgctca gatccagttt
    26881 gttcagctca acgttctgcc gtgcaatatc cagcgcttcc tgggaggtat caacgctgac
    26941 aacctggctg caaccgccca tcagtgccga tacggcgaaa ccaccggtat aggagaaaca
    27001 gttcagcaca cgtttatttt caacgtagcg gcgggtagcc aggcggctgt ctcgctggtc
    27061 cagatagtag cccgttttgt gtccgtgctg aatatccacc agcagcttca tgccgtgttc
    27121 ttcaatcggc agcagggcag gcggcaactc gccggtgacg aggccctggg tcagctccat
    27181 cccttctttt ttacgtaccg caacatcgct gcgatcgtaa atcgcacatt ccgggtacag
    27241 cgtttgcagg gcactaatta atgccgcgcg ctgatattct gcgccagcac tcagcagttg
    27301 cagcaccaga aaattaccga aacgatcgat agtaataccc ggcaggccat cagattctcc
    27361 ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc agccagtcac gccatttttg
    27421 tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata gactcagacg ggtcaaacgt
    27481 ccagacgcgc gcccggattt gcgaagctgg cgaataagcg ccgcgtgcta accattttcc
    27541 ctgatgatca acaatatcga tggtttcacc gaggctggct ttaccttcca tgcgggcaac
    27601 ggccccggaa aagacccacg gatggcgacg aagtaatgat ttttcgcgcc ctttggctaa
    27661 cactaaacgt acactcataa tttacttttc tgtcgatgca aagaaatggg cgtcattgtc
    27721 cggagttttc catggatttg caacgcgtcc attaaggata aggagagcag caatgtcgaa
    27781 agtctgcata attgcctgga tttacgggcg agttcagggc gtaggatttc gctacaccac
    27841 acagtacgaa gcgaaaagac tgggcttaac cgggtacgcc aaaaatcttg acgatggcag
    27901 cgtggaagtg gttgcctgcg gtgaagaagg gcaggtagaa aaattaatgc agtggttaaa
    27961 gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc agcgagccgc atcatccctc
    28021 gggggaatta accgattttc gtatccgtta aatacatttt accggttttg gcaggccagc
    28081 aattttggtg gcttgcttag ccgggccttt cgggaacagt cggtataaat agcggctgtt
    28141 acctttttct tcaccaaatt tattcgccat cgcttttacc agcatacgaa tcgccggaga
    28201 cgtattaaat tccaggtaga agtcacgtac aaaacgcacc acttcccagt gttctggcga
    28261 cagcgaaatc ccttcgttct ctgcaatcac cactgccagc ggttcactcc actggctgct
    28321 ttctttgaga tagccttcgg tatccgtttc tatctcttta ccttcgaaga tcagcataat
    28381 tattactacc ttaatcagac tggcggcagt gtaacaaaaa acaaagcccc gcataaagcg
    28441 aggctatgaa agtgttagcg ggtgagatta atcgcggctg gcgaagccca gaatgctcag
    28501 caggctgacg aagatgttgt acagcgaaac atacaggcta accgtggcac gaatatagtt
    28561 cgtttcaccg ccatgaatga tgttgctggt ttcaaacaag atagcgccag aggagatcag
    28621 aatgaagacc gcgctgatcg ccagatgcag agcaggcagt tgcaggaaga tattagcaac
    28681 cataccaatc agtaccacca caatacccgc catcagcata ccgccgagga acgacatatc
    28741 tttgcgggtg gtcagcacat atgcagagca gcagaagaac actaacgccg ttccgcccag
    28801 tgccatagcg attacgtcac ccattcctgc agacagatag gtgttcagaa ttggtccgag
    28861 gatataaccc agaaaaccgg taaaggcgaa tgcggagata atcccggtcg gcttattcgc
    28921 cgttttatag gtcaggaaca tcaaaccata catacccacc agcgtcagaa tcagacccgg
    28981 agatggcagc atcagcaccg tactggcagt tgcggtaatc gccgaaaagg ccagcgtcag
    29041 gctcagcaga aaataggtat tacgcagcac cttatgggtg ctaagcagtg atgtacggtc
    29101 atgtgaagaa ctaacaatac gatccattga gtcactctct atgacagatg taattaatta
    29161 agcagcataa tgataatgcg taagggcacc cagaagtttt acccatcttt acgcatttga
    29221 tctggaacag gtttaacagc ggattatcag gtcattaagc aaatataacg ccctgagaat
    29281 tccgacaggc aaaagaaaaa ggggttagca tttagctaac cccttagctt atttggcgga
    29341 agcgcagaga ttcgaactct ggaacccttt cgggtcgccg gttttcaaga ccggtgcctt
    29401 caaccgctcg gccacacttc cggaatgacg cgcactataa acatcccgat gcggcgtgta
    29461 aacccctaat ttgtttgttt gcctgaaaaa tagccaaaag tgcattgata gcgtgaaata
    29521 acagcagatt gatcatttca tcaccatgaa ttccttctct tttactcgtt tagcaaccgg
    29581 ctaaacatcc ccatcgcccg ggcaaagaaa aataggtcca tttttatcgc taaaagataa
    29641 atccacacag tttgtattgt tttgtgcaaa agtttcacta cgctttatta acaatacttt
    29701 ctggcgacgt gtgccagtgc agaaggatga gctttcgttt tcagcatctc acgtgaagcg
    29761 atggtttgcc ttgctacagg gacgtcgctt gccgaccata agcgcccggt gtcctgccgg
    29821 tgttgctagg agaagagacg tgcgatatga ataacgagga aacattttac caggccatgc
    29881 ggcgtcaggg cgttacccgg cgcagctttc tcaaatattg tagtctggct gccacgtcgc
    29941 tgggattagg cgcgggaatg gcaccaaaga ttgcctgggc gctggagaac aaaccgcgca
    30001 ttccggtggt atggatccac ggtctggaat gtacctgctg taccgaatct tttatccgct
    30061 ccgctcaccc actggcgaag gacgtcatcc tttccctgat ttccctcgat tacgacgata
    30121 ccctgatggc tgccgccgga acccaggcgg aagaagtctt tgaagacatc atcacgcaat
    30181 ataatggcaa atatatcctc gcagtagaag gtaatccgcc gctgggcgag caggggatgt
    30241 tctgtatcag cagcggtcga ccgtttattg agaaactcaa acgtgccgct gccggagcca
    30301 gcgcgattat cgcctgggga acctgcgcgt cctggggctg cgtgcaggcc gcgcgaccta
    30361 atccgacaca ggcaacgcct atcgacaaag tcatcaccga caaacccatt atcaaagtac
    30421 ctggctgccc gccgatcccg gatgtgatga gcgccatcat tacttacatg gtgacctttg
    30481 atcgcttgcc agatgtcgac agaatgggtc gtccactgat gttttatggt cagcgtatcc
    30541 acgataaatg ctatcgccgc gctcacttcg acgccggaga gttcgtccag agttgggatg
    30601 atgacgctgc ccgcaaaggt tattgcctgt acaaaatggg ctgcaaaggg cctaccacct
    30661 ataacgcctg ttcctccaca cgctggaatg atggcgtttc tttcccaatc cagtctggtc
    30721 acggctgcct gggctgtgcg gaaaatggtt tctgggatcg cggttcgttc tacagccgcg
    30781 tggtcgatat tccgcaaatg ggtactcatt ccaccgccga taccgtcggt ttaaccgcgc
    30841 ttggcgtggt ggcagcagct gttggtgtgc acgcagttgc cagcgccgtt gaccaacgca
    30901 gacgtcataa ccagcaacct acagaaaccg aacatcagcc aggcaatgag gataaacagg
    30961 catgagcact cagtacgaaa ctcagggata caccatcaat aatgccggac gccgcctggt
    31021 ggtcgacccg attacgcgca tcgaaggcca catgcgctgc gaagtgaata ttaacgatca
    31081 gaatgtgatc accaatgccg tctcctgcgg caccatgttt cgcgggctgg agatcatcct
    31141 gcaagggcgc gacccgcgcg atgcgtgggc gttcgttgaa cgtatctgcg gcgtctgtac
    31201 tggcgtacac gccctggctt cggtttacgc catcgaagat gccatcggta ttaaagtgcc
    31261 ggacaacgcc aatatcatcc gcaacattat gctggcaacg ctctggtgcc acgatcatct
    31321 ggtgcacttc tatcagcttg ccgggatgga ctggatcgac gtgttagatg cgctgaaagc
    31381 cgacccgcga aaaacctccg aactggcgca aagtctctcc tcttggccga aatcatcccc
    31441 tggctatttc ttcgacgtac aaaatcgcct gaagaaattc gttgaaggcg ggcagttggg
    31501 gatcttccgc aatggctact gggggcaccc gcagtacaaa ctgccgccag aagccaacct
    31561 gatgggcttt gcccactatc tcgaagctct cgatttccaa cgtgaaattg tcaaaattca
    31621 cgcggtcttt ggcggtaaaa acccgcatcc aaactggatt gtcggcggga tgccttgcgc
    31681 cattaacatt gacgaaagcg gcgcggtcgg ggcggtcaat atggaacgcc tgaacctggt
    31741 gcagtcgatt atcacccgca cggcagactt cattaacaac gtgatgatcc ccgacgcctt
    31801 agtcatcgtt cagttcaaca aaccgtggag cgaaatcggc acgggtcttt ctgataaatg
    31861 tgtcctcagc tacggcgcat tcccggatat tgccaacgac tttggtgaga aaagtctgct
    31921 gatgcctggc ggcgcggtga ttaacggcga cttcaacaat gtgctgccag tggatttggt
    31981 tgatccgcag caggtgcagg agtttgtcga tcacgcctgg tatcgttatc ccaacgatca
    32041 ggtcgggcgt catccattcg atggcattac cgacccgtgg tacaaccccg gcgatgtcaa
    32101 aggcagcgat accaacattc agcagctgaa tgaacaggaa cgctactcgt ggatcaaagc
    32161 gccacgctgg cgcggtaacg cgatggaagt ggggccgctg gcacgcacgt taatcgctta
    32221 tcacaaaggc gatgctgcga ccgttgagtc ggtcgatcgc atgatgtcgg cgttgaacct
    32281 gccgctttcc ggtatccagt caacgttggg ccgcattttg tgccgcgcgc acgaagcgca
    32341 gtgggccgca ggtaagttgc agtatttctt cgacaagctg atgaccaacc tgaaaaacgg
    32401 caatctcgcc actgcttcca ctgaaaaatg ggaacctgca acctggccga cagagtgccg
    32461 tggcgtcggc tttaccgaag cgccgcgcgg agcgttaggc cactgggccg ccattcgcga
    32521 tggcaagatt gatctctacc agtgcgtggt gccgaccacc tggaacgcca gcccgcgcga
    32581 tcccaaaggg cagattggcg cttatgaagc ggcgctgatg aacaccaaaa tggcgatccc
    32641 cgagcaaccg ctggagatcc tgcgtactct gcacagcttt gatccgtgcc tcgcctgttc
    32701 aactcatgtg ctgggcgacg acggtagcga gctgatctcc gtgcaggtgc gttaacagcg
    32761 aaggagaatc atcatgcaac agaaaagcga caacgttgtc agccactatg tctttgaagc
    32821 accagttcgt atctggcact ggctgacggt gttatgcatg gcggtgttga tggtcaccgg
    32881 ttactttatc ggcaagccgc taccttccgt cagtggcgag gcgacgtatc tgttctatat
    32941 gggttacatc aggttaattc acttcagcgc cgggatgatt tttaccgtgg tcttgctgat
    33001 gcggatctac tgggcttttg ttggcaatcg ttactcccgc gagctgttta tcgtgccggt
    33061 atggcgtaaa agctggtggc agggcgtgtg gtatgaaatc cgctggtatc tgtttctggc
    33121 aaaacgtccg agtgccgata taggccataa ccccatcgcc caggcggcga tgttcggcta
    33181 tttcctgatg tcggtcttta tgatcatcac cggttttgcg ctgtacagcg aacacagcca
    33241 gtacgctatt tttgcgccgt tccgttatgt ggtggaattt ttctactgga cgggtggcaa
    33301 ctcaatggac attcacagct ggcatcggct ggggatgtgg ctgattgggg cgtttgtgat
    33361 cggtcatgtc tacatggcgc tgcgtgaaga catcatgtcc gacgacacgg tgatctccac
    33421 catggtcaac ggctaccgta gccacaaatt tggcaaaata agtaacaagg agcgttcatg
    33481 agcgagcaac gcgtggtggt catggggctg ggcaacctgc tgtgggccga tgaaggcttc
    33541 ggcgtgcggg tggcggaacg gctgtatgcc cattaccact ggcccgagta tgtggagatt
    33601 gtcgatggcg gtactcaggg actgaacttg ctggggtatg tcgaaagcgc cagccatctg
    33661 ttgattctcg atgccattga ctacgggctg gaacctggaa cgctgcgaac ctatgccgga
    33721 gaacgcattc cggcctatct cagcgcgaag aaaatgagcc tgcatcagaa cagtttctcc
    33781 gaagtgctgg cgctggcaga tatccgcgga catctgccag cacatattgc cctcgtcggg
    33841 ctgcaacccg cgatgctcga cgactacggc ggcagcctga gcgaactggc acgggagcaa
    33901 ctgcccgctg cggaacaatc ggcgctggcg cagttggctg cgtggggaag tgtgccgcaa
    33961 ccggctaatg aatcgcgttg tctcaattat gactgtctgt cgatggaaaa ttacgaaggc
    34021 gttcgcttgc gccagtaccg gatgacacag gaggagcagg gatgagcaac gacacgccat
    34081 ttgatgcgtt gtggcaacga atgctggcgc gcggctggac gccggtcagt gaatcccgtc
    34141 ttgacgactg gcttacgcaa gtgccagacg gcgtggtgtt attaagcagt gacccgaaac
    34201 gcacgccaga ggtcagcgat aatccggtaa tgattggcga attactgcac gagtttcccg
    34261 actatacatg gcaggtggcg attgctgacc ttgagcagag cgaagccatc ggcgatcgct
    34321 ttggcgcctt tcgctttcct gccactttag tgtttaccgg cggaaactat cgcggcgtgc
    34381 tgaatggtat tcacccgtgg gcggaactga taaacctgat gcgcgggctt gtcgaaccgc
    34441 agcaggagcg tgcctcatga gcgaaacttt tttccatctg ctggggccag gaacgcaacc
    34501 gaacgatgac agtttcagca tgaatccact gccgatcacc tgtcaggtga atgatgaacc
    34561 gagtatggcg gccttggagc aatgtgctca cagcccgcag gtgattgcgc tgttaaacga
    34621 gttacaacat caactaagcg aacgccagcc gccgttgggc gaggtgctgg cagtcgatct
    34681 gttaaatctc aacgccgacg atcgtcactt tatcaatacg cttctcgggg aaggggaagt
    34741 gtcagtgcgc attcagcagg ctgacgacag taaaagtgaa atacaggagg cgatcttctg
    34801 cggattatgg cgggtgcgca gacgtcgcgg cgaaaagttg ctggaggaca aactggaggc
    34861 tggctgcgcg ccgctggcgt tgtggcaggc ggcaacgcaa aatctcttgc cgacagattc
    34921 gctgttaccg ccgcccattg atggcctgat gaatggccta ccgttggcgc atgagttact
    34981 ggcacatgta cgtaaccccg acgcgcagcc gcacagcatt aatctgacgc aattacccat
    35041 cagcgaggct gatcggcttt ttctctcacg tctctgtggg ccgggaaata ttcagattcg
    35101 taccattggc tatggcgaga gctatatcaa cgccacgggg ttacgccatg tctggcattt
    35161 acgctgtacg gacaccttaa aaggcccgtt actggaaagt tatgaaatct gcccaatacc
    35221 ggaagtggtg ctggcagcgc cagaagattt ggtcgactct gcgcagcggc ttagcgaggt
    35281 atgtcagtgg ctggcggaag ctgcaccgac gtaaaaagac ggtaagtatc gctttcagtc
    35341 ttatgaatat cgcaatcggc gaatacctct ggtcgtagag tttcaggata aagagggaga
    35401 tctaacatta tcgggttatt tttctctctt cgcctacagg agtgcgcatg tgggatgtca
    35461 ttgatttatc gcgctggcag tttgctctga ccgcgctgta tcacttttta tttgtacccc
    35521 ttaccttggg gctgattttt ttgctggcta tcatggaaac catttacgtg gtcaccggca
    35581 aaacaatcta ccgcgatatg acgcgcttct ggggtaagct cttcggtatc aattttgctc
    35641 ttggcgtggc taccggcctg accatggagt ttcagtttgg tactaactgg tcattctatt
    35701 ccaactatgt gggcgatatt tttggcgcac cactggcgat ggaagcatta atggccttct
    35761 tcctcgaatc cacctttgtc gggctgttct tcttcggctg gcaacggctg aataaatacc
    35821 agcacctgct ggtgacgtgg ctggtggcgt tcggttcaaa tctctctgca ttgtggatat
    35881 tgaatgccaa cggttggatg caatacccga ccggtgcgca ttttgatatc gacaccctgc
    35941 gtatggagat gaccagcttc agcgagctgg tctttaaccc ggtcagccag gtgaaatttg
    36001 tgcacaccgt catggcgggc tacgtgaccg gggccatgtt tattatggcg atcagcgcct
    36061 ggtatttact gcgcggacgg gagcgcgatg tcgcattacg ctcgtttgcc atcggttccg
    36121 tcttcggtac tctggcgatt atcggtaccc tgcaactcgg agacagttct gcgtatgaag
    36181 tcgcgcaagt acaaccggta aaactggcgg cgatggaagg ggagtggcaa acggaacctg
    36241 ctcctgcacc gttccatgtg gttgcctggc cggaacagga tcaagagcgt aacgcctttg
    36301 ccatcaaaat tcccgcgctg ctagggatcc tcgccactca ctcattagat aaacccgtgc
    36361 cgggtctgaa gaatttgatg gctgaaacct acccacgctt gcaacgcgga cgtatggcct
    36421 ggctgttaat gcaggaaata tctcaaggca atcgtgagcc gcatgtgttg caggcattcc
    36481 gggagctgga aggcgacctt ggttacggca tgttgctctc ccgctatgcg ccggatatga
    36541 atcatgtcac agccgcacag taccaggcgg cgatgcgtgg cgcgatacct caggttgcgc
    36601 cggtattctg gagtttccgc atcatggtgg gctgtggttc cctgctgcta ctggtgatgc
    36661 tgattgcgct tgtccagacg ctgcgtggca aaatcgacca gcatcgctgg gtgctgaaaa
    36721 tggcgctctg gagtttgccg ttgccgtgga ttgcgattga agccgggtgg tttatgaccg
    36781 agtttggtcg tcagccgtgg gcgatacagg acatcttacc gacatactcc gcgcactccg
    36841 ctttaaccac aggacaactg gctttctcac tgatcatgat cgtagggctt tacaccctgt
    36901 tcttaatcgc cgaagtctac ctgatgcaga aatatgcccg tctggggccg agcgcgatgc
    36961 agagtgaaca accgacgcag caacaggggt aaaggagaaa atcatgtttg attatgaaac
    37021 attgcgcttc atctggtggc tgctgattgg cgtgatcctg gtggtcttta tgatctccga
    37081 cggatttgac atggggatcg gctgtttgct gccgctggtg gcgagtaatg atgatgaacg
    37141 ccggatagtg attaatagcg ttggtgcgca ctgggaaggc aatcaggtct ggttgatcct
    37201 cgcaggtggg gcattatttg ccgcctggcc cagagtgtat gcagcggcgt tttccggctt
    37261 ttatgtggcg atgatcctgg tgctgtgctc gctgttcttc cgtccgctgg cctttgatta
    37321 tcgcggaaaa attgccgatg cacgctggcg taaaatgtgg gatgccggtc tggtcatcgg
    37381 cagtctggtg ccgccggtag tcttcggtat cgccttcggc aacttgttgc tcggcgtgcc
    37441 gtttgccttc acaccgcaat tacgcgtgga gtatctcggc agcttctggc aactgctgac
    37501 gccattccct ttattgtgtg gtttgctcag ccttgggatg gtgattttgc aaggtggcgt
    37561 ctggttacaa ctgaaaactg ttggtgtgat tcatctgcgt tcacagctgg cgactaaacg
    37621 cgctgcactg ttggtgatgc tgtgcttttt gctggcaggt tactggctgt gggtcggtat
    37681 tgatggcttt gtactgctcg cccaggatgc taacggtcct tccaatccgt taatgaaact
    37741 ggtggcagtg ctacctggtg cttggatgaa taatttcgtc gaatcgcccg ttttgtggat
    37801 cttcccgctg ctggggttct tctgcccatt gctgacggtg atggcgattt atcgtggtcg
    37861 cccgggttgg ggatttttga tggcatcatt gatgcaattt ggcgtgattt tcacggcagg
    37921 catcacgctg ttcccctttg tcatgccgtc aagtgtgagt ccaatctcca gcctgacgtt
    37981 gtgggacagc acttccagtc agctgacgct gagcattatg ttggtaatcg tgctgatatt
    38041 tttgcccatt gtgttgctct acactctctg gagctactac aaaatgtggg gacgcatgac
    38101 aacagaaact ctccgccgta acgaaaacga gttgtactaa ggagcaggaa catgtggtat
    38161 ttactttggt tcgtcggcat tttgttgatg tgttcgctct ccacccttgt gttggtatgg
    38221 ctggacccgc gtctgaaaag ttaacgaacg taggcctgat gcggcgcatt agcatcgcat
    38281 caggcaatca ataatgttag atatgaaaag cggaaacata tcgatgaaag cgatcttaat
    38341 cccattttta tctcttctga ttccgttaac cccgcaatct gcattcgctc agagtgagcc
    38401 ggagctgaag ctggaaagtg tggtgattgt cagtcgtcat ggtgtgcgtg ctccaaccaa
    38461 ggccacgcaa ctgatgcagg atgtcacccc agacgcatgg ccaacctggc cggtaaaact
    38521 gggttggctg acaccgcgcg gtggtgagct aatcgcctat ctcggacatt accaacgcca
    38581 gcgtctggta gccgacggat tgctggcgaa aaagggctgc ccgcagtctg ctcaggtcgc
    38641 gattattgct gatgtcgacg agcgtacccg taaaacaggc gaagccttcg ccgccgggct
    38701 ggcacctgac tgtgcaataa ccgtacatac ccaggcagat acgtccagtc ccgatccgtt
    38761 atttaatcct ctaaaaactg gcgtttgcca actggataac gcgaacgtga ctgacgcgat
    38821 cctctgcagg gcaggagggt caattgctga ctttaccggg catcggcaaa cggcgtttcg
    38881 cgaactggaa cgggtgctta atttcccgca atcaaacttg tgccttaacc gtgagaaaca
    38941 ggacgaaagc tgttcattaa cgcaggcatt accatcggaa ctcaaggtga gcgccgacaa
    39001 tgtctcatta accggtgcgg taagcctcgc atcaatgctg acggagatat ttctcctgca
    39061 acaggcacaa ggaatgccgg agccggggtg gggaaggatc accgattcac accagtggaa
    39121 caccttgcta agtttgcata acgcgcaatt ttatttacta caacgcacgc cggaggttgc
    39181 ccgcagtcgc gccaccccgt tattggattt gatcatggca gcgttgacgc cccatccacc
    39241 gcaaaaacag gcgtatggtg tgacattacc cacttcagtg ctgtttattg ccggacacga
    39301 tactaatctg gcaaatctcg gcggcgcact ggagctcaac tggacgcttc caggtcagcc
    39361 ggataacacg ccgccaggtg gtgaactggt gtttgaacgc tggcgtcggc taagcgataa
    39421 cagccagtgg attcaggttt cgctggtctt ccagacttta cagcagatgc gtgataaaac
    39481 gccgctgtca ttaaatacgc cgcccggaga ggtgaaactg accctggcag gatgtgaaga
    39541 gcgaaatgcg cagggcatgt gttcgttggc aggttttacg caaatcgtga atgaagcacg
    39601 cataccggcg tgcagtttgt aatgcataaa aaagagcatt cagttacctg aatgctctga
    39661 ggctgatgac aaacgaagaa ctgtctaatg cgtagaccgg aaaaggcgtt cacgccgcat
    39721 ccggtcactt tcagttttac tctttctcgg agtaactata accgtaatag ttatagccgt
    39781 aactgtaagc ggtgctggcg cgtttaatca caccattgag gattgcgcct ttaatattaa
    39841 cgccagcctg ttccagacgc tgcattgaca agctcacctc tttggcggtg ttcaagccaa
    39901 aacgcgcaac cagcaggctg gtgccgacag agcgtcccac gaccgcagca tcgctcaccg
    39961 ccagcatcgg cggcgtatcg acaatcacca gatcgtaatg gtcgttcgcc cattccagta
    40021 attgacgcat ccgatcgcgc atcagcagtt cagacgggtt aggtggcacc tgaccgcgag
    40081 taatcacatc aaagcctcct ttgccaaaat gctggatcac tttgttgagc tcatctttac
    40141 ctgccagata ttccgacaag ccatgttcat tactcacggt aaacaggtta tgcgaataac
    40201 cacggcgtaa gtcggcatca ataaataaca ctttttgatc ggactgggcg atcaccgctg
    40261 ccagagttga actgacaaac gttttaccac tgtctggcgt cgcaccggtg atcatcagaa
    40321 tgttattctc agtctccatc atagcgaaat gcagactggt tcgtagcgca cgtacggctt
    40381 ccacagcaga atccgccggg ttatccaccg ccaggaaggg gatattttta gtacgatggc
    40441 gctgctgatt agaaaataaa tttttcttac gcagacgggt gcgtttatcc agccactcgg
    40501 acattgggat agtggaataa acgctgatgc cgtgctcttc cagttgttcc ggggcttcta
    40561 caccacgacg caacatcgca cgcgccagca cggcactcac cgaaataaac aggccaagaa
    40621 taaaaccaag caccacattc aacgcttttt tcggtttcac tggctgcggc tgagtgactg
    40681 ccgggtcgat aatccgcacg ttaccaatgg cactggattt cgaaatactc aactcctgct
    40741 ggcggttaag taattgcaga tataccgcac ggcccgcttc tacgtcacga cttaaacgca
    40801 acacttcctg ttgggtggaa ggcattgccg atacccgctt attcaggcgt ttgcgttctt
    40861 gctccagcgt ctggcgtttt tccagcagcg cacgataagt tgggtgatct ttcttataca
    40921 gcagggagat ctctgcctcg cggaaggtca gctcattgag ttgattatca acgttcacaa
    40981 tctgctcaag aacggctttg gcttccaggt taaggtcaac cgaatcgcgc tgctggcgat
    41041 aaacgttgag tttttcttcc gcctggtcca gctcgctgcg cacttcaggt aactggctct
    41101 gtaagaattc aaggctttgt gaatcctgcg ccgcctggcg agcgatattc tgttgcaaat
    41161 agttgttagc gatgctgttc agaatacgag taatcaactg gggatcatca ccagtcatgg
    41221 taagttccag catcccgctt tctttactgc gttcgctaac ggtaaaggtt tcctgcaatg
    41281 cgttaatcgc ttccagttcg gtacgctggc tcaggacaaa ctgtgttcct ggtttggcct
    41341 taatgtccgc gatagtcagc gcaacgccat ctttttccag acgctggccg accataccat
    41401 tgacggtgaa ctcttcacct tccagtgtat agtggccgtt ttccccaacc gtgagtgtca
    41461 gttgctgatc ctgaccattc agttgtggaa tatgcatcca gctgatcgcc agctcacctg
    41521 gtttttcttt ggttaatctc gcccagccgc gacccacaat cggaaaatac ttctgctcaa
    41581 ctatgtcgcg cagattcagt tcagcaatgg ttttaccgag aatcatgcgc gattgcagca
    41641 gttggatctc cggtgcagac tcgggcgatg agttagggat catatcgctc aggccgctga
    41701 gaatggcgtt gccctgtttt tgctcaacct ggaccagagt atctgcctga taaattggtg
    41761 tgcttaacag cgatcagcgt gaataacgcg gtcacgctga taataaactt acggtgatcc
    41821 cataactcgc cgaccagacg aagcagatcg atctcatttt cctgagtgct gcctagtggc
    41881 gtattcatat ttttagttgt catcgtattt tataaactca aattaccggc tgaggcgctt
    41941 cgcccattcc tgactggcgc gctccaacat accgtagaca tgttcaaatg cgtcctgact
    42001 tttacgatag ggatccggga tctctttctg ttccagccat tgcccaaaca gcattgtttt
    42061 cccgcggacc tcgggagcaa ttgcggtaac ctgagcaata tgttccgact ccattgccag
    42121 aatcagatcg taatttcgtg ccatctcagc agtgagctta cgtccggcat gtccttcaag
    42181 agatacgcca tgattagcgg caacatctgc cgctgtcgca tctgcagggt gttttaccag
    42241 accatgaaca cctgccgatt tgacttttac gcccggcaga cgtttacgca gcaagcgctc
    42301 gccaatgggc gaacggcaaa tgttgccggt acaaaccact aggattgagt taaattttag
    42361 ttgggccatg tttgaatcca ccgtgaagtc tctgtcaggt catgcacccc ggaaatcgtc
    42421 ggtaccagca gagaaatcac acggttccaa cgagccagcg gcgcggtagt gacataaaca
    42481 atgtcgtaag gttcaagctg gaattccgtt ccgagtatca ttgccgatgc gtccttcgca
    42541 ttcagctgat aaatattggc gattttgcca ttctgcttgt tttgcgttgc acgaataaca
    42601 aaaatccccg tcgcatcagc gacatcctga ttcataccct cagcgttacc cagcgcttcc
    42661 gccagggtca taccgctgcg gtccattttc agcgtgctct gtttacccac ttcacccatc
    42721 acgaagactt tcagatcatc gttgcgcgga ataaataaaa tatcgcccgg gtgcagcagt
    42781 ttgttctggc gcaagtcgcc acgctgcatc agcgcataga ggttcacttt ggtctttacg
    42841 ccattctggg tgagcaccac gttgcgccag tctgcatctg cggtcagacc gcccgcggcg
    42901 ttaatcgcat ccatgatggt cagaggaata ttggtaatcg gctgctgacc cgatttgctg
    42961 acttcgccgg tgacgtacgc tttttgcgat cggaacgccg cgacgctgac atcgacttgc
    43021 gggctttcaa tgactgaatc gagacgcgct gtaatctcgt tgcgtacctg ggtaagtgtt
    43081 ttcccggcca ctttcaggcg accaatatag gggtagaaaa tcgcaccatc ggcgttaacc
    43141 cagttgccgg tatcactggc gctacggtat tgccctgcgg gggtggttag ttccgggtga
    43201 tcccagaccg taaccatcaa cacatcacca atcccgatac gatattcata gtttgcgatt
    43261 tgctgctcca gctccgggtt agcctgcgac ataatcggtt tggcgcgcag ctgatcaatc
    43321 aatcctggcg tgacgggata gatattgacc attttgtcca ggtcatactg attgtcaggt
    43381 agttcaatga catccttgtt actggtcgat aaattctgac caggaacaag ggtacatgct
    43441 gtcaaagaca gaacagccaa tgtcagtacc gaaaacttaa aaatgttctt tttcattggt
    43501 ttatatttat cagtcaatgt aatgttatga attacgtcag gttattgtcc aaccggtact
    43561 tctcgctctg ccgtcattgg atcgagctgg tatttgcgtg acagcatttg tccaccatca
    43621 cgcgtcaatg gtgtccacga aaccaccgcg cggttgcggt taggtccaat ggtcatcaag
    43681 tcgaacggaa tcgagatata aaaaccttta ctaaagccgc cttcgccgta gtcatcttta
    43741 gatacgttac tgattgccgc ccatacccct accgccacgc cgttgtcaaa gcgtttggcg
    43801 acgtcgatcg ttgcgccttt atcttttgcc agatattgcc caacgctaag tttcatcagt
    43861 acgccattga gcgtcggcgg gttccagtaa gccgtcacga aacctgtggg cgtggaataa
    43921 tcggtgaagc gcatcatgtt gtcccagtca cgctgcttca cgtagttaac gtccacaccc
    43981 agcgcccagc tggcatctag cgggcgataa agaagctcgg aaccgacacc ggcatacatc
    44041 gtttccagat aaccgccata cacctggcca tagaaaccat tgcctaagtc ggcaaagtaa
    44101 ttcgcttgca agttgttgag ataaacgtca ttgcgaacgt aatcacggat atgcgtgcgc
    44161 acgcggggca gggtagagtc cgcgggtaac agtgaagact taaacttgtc gtagttgttg
    44221 taaatattgg tgaaaatacc gccatcaagc agcaggtggt cggtaaacca gtagcgggcg
    44281 ctggacatca gccccagctg gaacatatag aaatcttccg ggccgcccag cgactgtgaa
    44341 agtgttgggt tgaaagagta gctaaagcga tcttcacgaa tacggtagcc ccgaccaaag
    44401 gcagaaagat cttctgcttc aacacgttgt tgttgcagtt gctctgattt accaggcgct
    44461 gtgccggcca gctgtttgcg caggctggct acgtcggttt cggtagtcac catcgccata
    44521 tgctcgcgct tttgcgtcac gctaatcttc tcaacgcctt gcggcaggtt attcaccaga
    44581 atccggttgg cacgatcgac cgcttcgcga gagtcacggt atttgtattg ctgaccagac
    44641 atatacagcg tcttatcgcg tagctgaatt tctggcgcgt caaagcccgc gttatacttc
    44701 agggcggtaa gttggtttgc taccgtggtg tactgcaaac cttcagactc tggcgcaggc
    44761 tgccaggccg gttttggcgt atcgcgtagg gcagggcgca gatcgttgaa attggtccgt
    44821 aaagtgaaac caaacatcaa cgtgtttccg cgttcataac tgaggttgag gtctgcccag
    44881 ctggcagcgc gataaactgc accgacgttg aaatggcttg cctgaggcag tttgccagcg
    44941 aaatcattct ggtaattgtt gccgtcgtat tcgagtttca gacgcagggg attccacggc
    45001 gtttgatact caatgccgcc aaagatggaa gccgggccac gaaagatatc gctaaagctg
    45061 atatcgcccg catcgtgaga ctctgcgcga tgacaatatt tatcgctcac ccggcaaaac
    45121 gggttggtaa tattgcccgc attaccggcg tagccccatg ccatcccgag ggtgaaatca
    45181 aatggccccg cttgcttgct ggccaccaga tattcaccgt caaacaaacc cgtaccagca
    45241 atatcacgtt taccaaacgc cacttgcggt agccagtaac cttcttccca caggcgaagt
    45301 ttaaaatcga atgatttgtc tttgtatgac tgatcgccgc tgaaatcttc ccactggcta
    45361 tatttgcgtg tgcgcacatc cgtataacga atggtgcctt ccagccatgg gaacagcgcc
    45421 acggaggtgg agtagaaccg gtattgatcg ttatcccggt agttgacgct gaattcacct
    45481 tccggtgcga tgcgcgcatt tggcatctgc aacaatcctg tgccgccaaa gtctgattgc
    45541 gacgaaccca gcggatccgg gtaggttaat acttcagcat gacaggcact ggagacggca
    45601 atggccaggc agcttaaaag ataagaattc ttcttcatca gtcaggaaca cgttgcgtta
    45661 aaacagacac aatctggtca tttaaatcag catatttttc gggcagtaca tgcgcagaaa
    45721 agcccaacca aagctggctg ccaggcggtg gttccacatg tcttttattc cacagggcaa
    45781 caggggcgac gacagtctca ccctcaggcg taatcaccat aacgttattc ttgtccgcgc
    45841 ctgcaaggcg ggggtgatct tgcagataat cggtaacgct acggcctgcc tgccagggca
    45901 attgcccagc cccagaaacc gcacccagca gagtaatagt caccggacgc tgtacggtat
    45961 agagcgtata gtccccgacc aacgggggat tgctgttctc atccactcgt acaaaatcag
    46021 ggtccagttt gaccggtaag cgaccagtga tattgagatt gagtaactgc tggcgcacgg
    46081 atttaatcgt tgccgccaca tcgtcgtcag cttctgcttc ccaggaagca agctgtgcca
    46141 tgacatgttg gtagtcttta agcgctttag ctttcgccgc agagtcggtc agcaaggcac
    46201 caggccacca gaggcgatcg cgcagttgcg gttgtgtcac caactggaca acattttcca
    46261 cggggccaac ggaaagtgtc tgttgttcgc taggcagata aatagtcacc gttccttgcg
    46321 caaaggcatg gggtgtcatt acgtaaagta cgctggcaat gaaatacgac tgtaatttat
    46381 tcatggtgcg ggtttaagaa acgtcatttc tacgggaata acccctgcgc ctaacatttg
    46441 ccgactttga cgaacctggc cagtagccga atcgacccag aatgtgtttt gccactgttt
    46501 ttctggcagc gtggaagtca cctcttcctg ccagacatta cacagcacat tgtgacccgc
    46561 aatattcagc gtctctaatc cagcaaagga aaaagtagaa cttagggtcg cagagcgaaa
    46621 atggttgtct tcactccaga gaatatcgcg cgtccagcgg ctgccatctt ttattgccag
    46681 cgcgttacgc agagggtccc gcccgggatt tgtcacttcc agcagattat tattaagttt
    46741 gacggtcttt aaaagacgtc cattgtgggt aaccagcatg gcgttatcct gggataacca
    46801 tttgctttgt tcttgttcta tatatcccag taccacaaag attcgttgcc cttcattaag
    46861 gcgtaaatac atcgtggaat aaggcaacgc ctggatctgc tgatccgcta cagtgatatc
    46921 ctgattatcg aaaaggctgg cgcgaaatgt atcgaccata ctttgctggc tgtgcgtaca
    46981 tcccgcaaga aatagtgcg
//
LOCUS       DBKEKI010000018        45825 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.018, whole genome shotgun sequence.
ACCESSION   DBKEKI010000018 DBKEKI010000000
VERSION     DBKEKI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.018"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(115..1119)
                     /gene="cra"
                     /locus_tag="ACUQPX_000950"
     CDS             complement(115..1119)
                     /gene="cra"
                     /locus_tag="ACUQPX_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HHV9055727.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1225..1287)
                     /locus_tag="ACUQPX_000951"
     CDS             complement(1225..1287)
                     /locus_tag="ACUQPX_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HHV9055728.1"
                     /translation="MRNLQPNMSRWAFFAKSVGT"
     gene            complement(1299..1790)
                     /gene="ilvN"
                     /locus_tag="ACUQPX_000952"
     CDS             complement(1299..1790)
                     /gene="ilvN"
                     /locus_tag="ACUQPX_000952"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HHV9055729.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGANVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1793..3517)
                     /gene="ilvI"
                     /locus_tag="ACUQPX_000953"
     CDS             complement(1793..3517)
                     /gene="ilvI"
                     /locus_tag="ACUQPX_000953"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HHV9055730.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3636..3680
                     /locus_tag="ACUQPX_000954"
     CDS             3636..3680
                     /locus_tag="ACUQPX_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055731.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3835..4779)
                     /gene="leuO"
                     /locus_tag="ACUQPX_000955"
     CDS             complement(3835..4779)
                     /gene="leuO"
                     /locus_tag="ACUQPX_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HHV9055732.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPS
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5439..5525
                     /gene="leuL"
                     /locus_tag="ACUQPX_000956"
     CDS             5439..5525
                     /gene="leuL"
                     /locus_tag="ACUQPX_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HHV9055733.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5618..7189
                     /gene="leuA"
                     /locus_tag="ACUQPX_000957"
     CDS             5618..7189
                     /gene="leuA"
                     /locus_tag="ACUQPX_000957"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HHV9055734.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7189..8280
                     /gene="leuB"
                     /locus_tag="ACUQPX_000958"
     CDS             7189..8280
                     /gene="leuB"
                     /locus_tag="ACUQPX_000958"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HHV9055735.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8283..9683
                     /gene="leuC"
                     /locus_tag="ACUQPX_000959"
     CDS             8283..9683
                     /gene="leuC"
                     /locus_tag="ACUQPX_000959"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HHV9055736.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKIMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9694..10299
                     /gene="leuD"
                     /locus_tag="ACUQPX_000960"
     CDS             9694..10299
                     /gene="leuD"
                     /locus_tag="ACUQPX_000960"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HHV9055737.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10348..11526)
                     /gene="setA"
                     /locus_tag="ACUQPX_000961"
     CDS             complement(10348..11526)
                     /gene="setA"
                     /locus_tag="ACUQPX_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HHV9055738.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11628..11759)
                     /gene="sgrT"
                     /locus_tag="ACUQPX_000962"
     CDS             complement(11628..11759)
                     /gene="sgrT"
                     /locus_tag="ACUQPX_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of D-glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HHV9055739.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11848..13503
                     /gene="sgrR"
                     /locus_tag="ACUQPX_000963"
     CDS             11848..13503
                     /gene="sgrR"
                     /locus_tag="ACUQPX_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HHV9055740.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEMPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13537..13636
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13667..14650
                     /gene="thiB"
                     /locus_tag="ACUQPX_000964"
     CDS             13667..14650
                     /gene="thiB"
                     /locus_tag="ACUQPX_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HHV9055741.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESNLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14626..16236
                     /gene="thiP"
                     /locus_tag="ACUQPX_000965"
     CDS             14626..16236
                     /gene="thiP"
                     /locus_tag="ACUQPX_000965"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HHV9055742.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16220..16918
                     /gene="thiQ"
                     /locus_tag="ACUQPX_000966"
     CDS             16220..16918
                     /gene="thiQ"
                     /locus_tag="ACUQPX_000966"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HHV9055743.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(17032..17796)
                     /locus_tag="ACUQPX_000967"
     CDS             complement(17032..17796)
                     /locus_tag="ACUQPX_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HHV9055744.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17882..18760)
                     /gene="araC"
                     /locus_tag="ACUQPX_000968"
     CDS             complement(17882..18760)
                     /gene="araC"
                     /locus_tag="ACUQPX_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HHV9055745.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19099..20799
                     /gene="araB"
                     /locus_tag="ACUQPX_000969"
     CDS             19099..20799
                     /gene="araB"
                     /locus_tag="ACUQPX_000969"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HHV9055746.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRHYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20810..22312
                     /gene="araA"
                     /locus_tag="ACUQPX_000970"
     CDS             20810..22312
                     /gene="araA"
                     /locus_tag="ACUQPX_000970"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HHV9055747.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGDFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVFGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22512..23207
                     /gene="araD"
                     /locus_tag="ACUQPX_000971"
     CDS             22512..23207
                     /gene="araD"
                     /locus_tag="ACUQPX_000971"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HHV9055748.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIMLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23282..25633
                     /gene="polB"
                     /locus_tag="ACUQPX_000972"
     CDS             23282..25633
                     /gene="polB"
                     /locus_tag="ACUQPX_000972"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HHV9055749.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNNELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQKG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25798..28704
                     /gene="rapA"
                     /locus_tag="ACUQPX_000973"
     CDS             25798..28704
                     /gene="rapA"
                     /locus_tag="ACUQPX_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HHV9055750.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28716..29375
                     /gene="rluA"
                     /locus_tag="ACUQPX_000974"
     CDS             28716..29375
                     /gene="rluA"
                     /locus_tag="ACUQPX_000974"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HHV9055751.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29492..30307)
                     /gene="djlA"
                     /locus_tag="ACUQPX_000975"
     CDS             complement(29492..30307)
                     /gene="djlA"
                     /locus_tag="ACUQPX_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HHV9055752.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKLDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30562..32916
                     /gene="lptD"
                     /locus_tag="ACUQPX_000976"
     CDS             30562..32916
                     /gene="lptD"
                     /locus_tag="ACUQPX_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HHV9055753.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEALGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32969..34255
                     /gene="surA"
                     /locus_tag="ACUQPX_000977"
     CDS             32969..34255
                     /gene="surA"
                     /locus_tag="ACUQPX_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HHV9055754.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDTTLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRGRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34255..35244
                     /gene="pdxA"
                     /locus_tag="ACUQPX_000978"
     CDS             34255..35244
                     /gene="pdxA"
                     /locus_tag="ACUQPX_000978"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HHV9055755.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATFLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35241..36062
                     /gene="rsmA"
                     /locus_tag="ACUQPX_000979"
     CDS             35241..36062
                     /gene="rsmA"
                     /locus_tag="ACUQPX_000979"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HHV9055756.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDHFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36065..36442
                     /gene="apaG"
                     /locus_tag="ACUQPX_000980"
     CDS             36065..36442
                     /gene="apaG"
                     /locus_tag="ACUQPX_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HHV9055757.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVHGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36449..37291
                     /gene="apaH"
                     /locus_tag="ACUQPX_000981"
     CDS             36449..37291
                     /gene="apaH"
                     /locus_tag="ACUQPX_000981"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HHV9055758.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPVPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37369..37848)
                     /gene="folA"
                     /locus_tag="ACUQPX_000982"
     CDS             complement(37369..37848)
                     /gene="folA"
                     /locus_tag="ACUQPX_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HHV9055759.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(38039..39900)
                     /gene="kefC"
                     /locus_tag="ACUQPX_000983"
                     /pseudo
     CDS             complement(38039..39900)
                     /gene="kefC"
                     /locus_tag="ACUQPX_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
     gene            complement(39893..40423)
                     /gene="kefF"
                     /locus_tag="ACUQPX_000984"
     CDS             complement(39893..40423)
                     /gene="kefF"
                     /locus_tag="ACUQPX_000984"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HHV9055760.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40471..41865)
                     /locus_tag="ACUQPX_000985"
     CDS             complement(40471..41865)
                     /locus_tag="ACUQPX_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9055761.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41924..42211)
                     /gene="fixX"
                     /locus_tag="ACUQPX_000986"
     CDS             complement(41924..42211)
                     /gene="fixX"
                     /locus_tag="ACUQPX_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HHV9055762.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42208..43494)
                     /locus_tag="ACUQPX_000987"
     CDS             complement(42208..43494)
                     /locus_tag="ACUQPX_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HHV9055763.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEASAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTSENTLSLGLVCGLHHLHDAKKSVPQMLEDFKHHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43545..44409)
                     /locus_tag="ACUQPX_000988"
                     /pseudo
     CDS             complement(43545..44409)
                     /locus_tag="ACUQPX_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(44424..45194)
                     /locus_tag="ACUQPX_000989"
     CDS             complement(44424..45194)
                     /locus_tag="ACUQPX_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HHV9055764.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45666..>45825
                     /locus_tag="ACUQPX_000990"
     CDS             45666..>45825
                     /locus_tag="ACUQPX_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="catalyzes the exchange of L-carnitine for
                     gamma-butyrobetaine in carnitine metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HHV9055765.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGW"
BASE COUNT        11246 a        12511 c        11783 g        10285 t
ORIGIN      
        1 ccatttatca ataatagtca ggaatagtct tatttacttt aaggatattg atgtccagtc
       61 ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg cggcttagct
      121 acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac caggttttgg
      181 cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg cgacatcgcg
      241 gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt cgttatcgcc
      301 aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg tgacatccat
      361 cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg gatgcgtttc
      421 cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg catacaggaa
      481 atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac gcaggaagct
      541 gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa acttacgtaa
      601 ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg tgaaatgttc
      661 acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc agcgttgata
      721 aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct gacgctgtaa
      781 aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg caatcagcag
      841 ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg tatagctggt
      901 gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac gaagcccagc
      961 tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt tttcaacggt
     1021 tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg cagtggtccg
     1081 cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt gcgtaaaatg
     1141 taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa gcgcagaaaa
     1201 ttaactgcgg caactgcttt tatttcaggt tcccactgat ttcgcaaaaa aagcccaacg
     1261 tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat tttatcgccg
     1321 cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac atcgcgaatc
     1381 gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt ataaagcgag
     1441 ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac ttcgtcacgc
     1501 ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatt cgcgccctgc
     1561 cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc gatctgctca
     1621 agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg atcgtcggtt
     1681 ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc aatcacgcgg
     1741 gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat aatcaggttc
     1801 tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc atcgggtaga
     1861 cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg cgcacctgtt
     1921 ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg atcccgacgt
     1981 gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa gattgtgaat
     2041 gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag cggttattga
     2101 gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc tggatgttca
     2161 tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac gccattttga
     2221 cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa ttgatccagc
     2281 gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc ccgacatcgg
     2341 acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc gcctgcggtt
     2401 taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac tgttcaatct
     2461 gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc gacaggagtt
     2521 caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc gcagtcacgg
     2581 ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt gggcagtact
     2641 ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa atcacatccg
     2701 cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatt cccagtgcct
     2761 gacgatgcgt tgccggaaac gcccccagcc ccatcaatga ggaaacaacg ggcagattca
     2821 acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc ccaccgccta
     2881 cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta atttgccctt
     2941 tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg cagacatagg
     3001 gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact ggtcctgggc
     3061 gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct tccgtttgct
     3121 taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg cactcctgaa
     3181 aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact aatggaatgg
     3241 aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccctgga cccgacgtta
     3301 ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata tgcaccgccg
     3361 cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat gcatcgtaaa
     3421 tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga tcgataagcg
     3481 atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact gtttgacgga
     3541 aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca gccagagaca
     3601 taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat aaaatcctcc
     3661 attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca gcaatttctc
     3721 tattttttta attaatccta aaatagagag tccacacatt cagaataaaa ttctacattc
     3781 aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat tcagttagcg
     3841 tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc gcccggcagc
     3901 ttcatgccag gagagataac aggttctgct gttttgtttt aacggcagcg gtaatacctg
     3961 taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag cgaccaaatg
     4021 cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg cgatactggc
     4081 ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat cgagcgaaac
     4141 cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa ttgttggatg
     4201 atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg taaattcagg
     4261 acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca gctgatgttc
     4321 agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa tctgctcaat
     4381 gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac aaagatgaaa
     4441 tacacgttca ctgctcgcgg gttcaaaacc tgaactaggc aattcatttt gtactagttg
     4501 caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt gaataccacg
     4561 gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa cagcgttact
     4621 gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt gctcctgcat
     4681 cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg gcttgcttaa
     4741 ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca ctgtcacact
     4801 taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg atttatcatg
     4861 catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga tacgggttct
     4921 gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa taatccataa
     4981 tgtttttgtt gcgaaaacaa tctaatcata actaccgcga atactcaatc atctacaaaa
     5041 tggattaaat gtgcttattt aataattaat ttatatattt aatgcattaa ttcttaacat
     5101 taattgatca ataatattca ccaaatcaat atcaaaaaaa atcgcaaaac atataattca
     5161 atacaaatca tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt tcctcgttga
     5221 ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg tagttaagca
     5281 gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta cggatgcaga
     5341 actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt gtatccagta
     5401 actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc gttcgcttta
     5461 tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc ggcatccagc
     5521 attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt ttttatgccc
     5581 gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag tcattatttt
     5641 cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg tgaaagaaaa
     5701 actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag tcggtttccc
     5761 cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg ttaaaaacag
     5821 ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg ccgaatccct
     5881 gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa tgcacatcgc
     5941 caccaagctg cgcagcaccc tggacgaggt gatcgaacgc gctatctata tggtgaaacg
     6001 cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc gtacacccat
     6061 tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca ccatcaacat
     6121 tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca gcggcctgta
     6181 tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg acgatttagg
     6241 cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg tggaaggtgc
     6301 aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca tcatggcgat
     6361 taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccagg agatatggcg
     6421 caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca aagccattgt
     6481 tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc tgaaaaaccg
     6541 cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc agctgaatct
     6601 gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg ggtataaaga
     6661 aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg acaaaaaagg
     6721 tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc aagaagagcc
     6781 ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata tcgccaccgc
     6841 cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg gtaacggtcc
     6901 ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg aactggtgaa
     6961 atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg atattgtcgc
     7021 caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg tcgagtcctc
     7081 cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag tcgaaaaaga
     7141 gttgcaacgc aaagctcaac acaacgaaaa caacaaggaa accgtgtgat gtcgaagaat
     7201 taccatattg ccgtattgcc gggggacggt attggtccgg aagtgatgac ccaggcgctg
     7261 aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag ccattacgat
     7321 gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac ggttgaaggt
     7381 tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg ggaacattta
     7441 ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca cttcaaatta
     7501 ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg tccgctgcgt
     7561 gcagacattg ccgctaacgg cttcgacatc ctgtgcgtgc gcgaactgac cggcggcatc
     7621 tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc gtttgatacc
     7681 gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga atctgctcgt
     7741 aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc ctctatttta
     7801 tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact ggcgcatatg
     7861 tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga cgttctgctg
     7921 tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac tggctcgatg
     7981 ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga accggcgggc
     8041 ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca aatcctgtcg
     8101 ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc cattgaacgc
     8161 gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg tggcgctgcc
     8221 gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga aggggtgtaa
     8281 tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac gaagccgaaa
     8341 acgaaacccc gctgttatat atcgaccgcc acctagtgca tgaagtgacc tcaccgcagg
     8401 cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa accttcgcca
     8461 ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt gaaatggcgc
     8521 gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag ctgtatgacc
     8581 tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc gtcaccttgc
     8641 cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg tttggcgcac
     8701 tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa accctgaaac
     8761 agggccgtgc gaagatcatg aaaattgaag tccagggcaa agccgcgccg ggcattaccg
     8821 caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc accgggcatg
     8881 tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt atgaccctgt
     8941 gcaatatggc gatcgaaatg ggcgcaaaag ccggtctggt tgctcctgac gaaaccacct
     9001 ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac gacgccgttg
     9061 cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt gtcactctgc
     9121 aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag gtgatttccg
     9181 tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt gcgtcggcag
     9241 aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa gtggctatcg
     9301 acaaagtgtt tatcggttcc tgtaccaact cgcgcattga agatttacgc gcggcagcgg
     9361 agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt cccggctctg
     9421 gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt gaagccggtt
     9481 ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac cgtctgaatc
     9541 cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag gggcgcggcg
     9601 ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc ggacatttcg
     9661 ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat caaacacaca
     9721 ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat cccgaaacag
     9781 tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga ctggcgtttt
     9841 ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc gcagtatcag
     9901 ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg tgagcacgcg
     9961 ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt tgctgacatc
    10021 ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga tgcagaagtg
    10081 gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt ggatctggaa
    10141 gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc cttccgccgc
    10201 cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga cgacgctatt
    10261 gcgtcttatg aagcaaaaca acctgcgttt atgaattaga ccccttgccc ggtcaaatga
    10321 ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg ttaaaaataa
    10381 tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc aactttgtgc
    10441 aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt tggtaaataa
    10501 ggtggtagct gcccccgctc ttccaggcat taaatcctga aaccatagca tcccaatacc
    10561 cgcaacaatg ccgataaata cagcgttaaa aagttgcagc gtcatcaacg ccatacggct
    10621 atgaaagaaa atcaatccag tgtaaaacag cactcctgcc accactgcaa tgaccatcat
    10681 tcgccgctta ccataacgtt tgacatagta gccagccaga atcattgctg gtatttccag
    10741 tccagctgcc gtccccatca ggaaacccgc cagtttgtct ggcaatccta aatcgctact
    10801 gatccacaac ggcatatcaa taatgtacat agtgttgcag gtccacatta acgtcgaggc
    10861 gacaaataac atccgtacgt tactatcctg ccagtcacct tgcattgata aagcattttc
    10921 cgacggcagt tctacccgcg ccacagacgg aagcataaat gcaatcaata ccagactgag
    10981 tgtgaatatc ccggcggcaa tcgaaaacat cactgtaaag ccgtaattca acgccagcat
    11041 aaaggccagc ggtggaccga ttacccatgc cagagaaagc tgcgcacgca tcaccgagct
    11101 aaacatcacc acttctcgcg ccgagttatc cgcgtattcc cgcgccagag caaataactg
    11161 tggcattgcc gtattggcca gagatgccag aagcacacca caggtgataa gcgtcagata
    11221 atgacgatta aatgcaaaca gtagcgcatt gccaatagcc atcaaacagc aaaatataat
    11281 cagttttcgc cgatcgccct gactgtcaga acgttttgcc aaccagaggc ttacgccgat
    11341 cccagcaata gcattcaccg tataaaagag tccgatccag aaaggttgcg cgccaacctc
    11401 acgactcaga aataagctca atgtaggagc ctgtagcgcc ccggccaccc ccatcataaa
    11461 agcgaccagc ataaatgccg cgtaaacacc gttcatacgg cgagccatcg tcattatcca
    11521 gatcatacgt tcccttttta gcgcggcgag aataaaaaaa accagcaggt ataatctgct
    11581 ggcgggtgat tttacaccaa tactcagtca cacatgatgc aggcaagtca actttcagaa
    11641 ttgcggtcat cccactgcat cagtccttca agcatggtta atcgttgtgg gacgcttaac
    11701 caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa ctgacgcatg
    11761 gggcacctcc ttgcttcatc gttaggaatt attgacttaa tatagggaaa ataaaattgc
    11821 tgtcttttgc gcaggagttc cccttttatg ccatctgctc gtctgcaaca acagttcatc
    11881 cgcctgtggc aatgctgcga gggtaaatcg caggacacaa cgctcaacga actggcagcg
    11941 ttattgagct gctcgcgtcg tcatatgcgt accctgctca acaccatgca ggatcgcggc
    12001 tggctgacgt gggaagcgga agtcgggcgc ggtaaacgct cgcgtctgac attcctctat
    12061 accgggctgg cgcttcagca acagcgggcg gaagacctgc tggagcagga tcgtatcgac
    12121 caactagtgc agttggttgg cgacaaagcg actgtgcggc aaatgctggt ttctcatctg
    12181 ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg tccgttgcgt
    12241 aatctgctac ctggcagcgc attgcgccgt tccgaaaccc atattgcccg gcaaatcttc
    12301 agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg aagcagacat cgcccaccac
    12361 tggcagcaaa tatcaccgct tcactggcgt ttctttttgc gtccaggagt ccattttcat
    12421 catggtcgtg aactggaaat ggacgacgtg atcgcctctt taaaacgaat caatacgctg
    12481 ccgctctatt cgcatattac ggacattgtg tcgccgacgc cctggacgct ggatatccat
    12541 ctcacgcagc cggaccgctg gttgccgtta ctgctggggc aagttccggc gatgatcctg
    12601 ccgtgcgaat gggaaaccct cagtaacttt gccagccatc ctattggcac cggtccgtat
    12661 gcggtgattc gcaacagcac caatcaactg aaaattcagg cattcgatga cttcttcggt
    12721 taccgggcat taatcgacga agttaacgtc tgggttctgc cggaaattgc cgacgagcca
    12781 gccggtggtc tgatgctaaa aggtccacag ggcgaggaaa aagagattga aagccgcctg
    12841 gaggaaggtt gctactattt actgttcgac agccgcaccc atcgcggggc gaatcagcaa
    12901 gtcagggact gggtaagcta tgtgctttct ccaactaatc tggtctattt cgctgaggaa
    12961 cagtaccagc aactgtggtt cccggcttat ggactgctcc cccgttggca ccatgcccgc
    13021 accataaaga gcgaaaaacc ggctggcctg gaaagcctca ccctgacctt ttatcaggat
    13081 cacagtgagc atcgggtgat tgccgggatc atgcagcaga ttctggcaag tcaccaggtc
    13141 acgctggaaa tcaaagagat cagctacgat cagtggcatg aaggagagat cgagagcgat
    13201 atctggctta atagcgccaa ctttacgctg ccgctggatt tttcgctgtt cgcgcacttg
    13261 tgcgagatgc cgctgctcca acactgtatt ccgatcgact ggcaagccga cgccgcccgc
    13321 tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc aactggtcgc cagtaaagca
    13381 atggtgccgc ttatccacca ctggctgatc attcagggac aacgcagtat gcgcggcctg
    13441 cgtatgaata ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc accggatcca
    13501 tgattgctgg tagataacaa aatcactaca ctaacgccgt tctcaacggg gtgccacgca
    13561 aacgcgtgcg ctgagaaaat acccgtcgaa cctgatccgg ataacgccgg cgaagggatt
    13621 tgaggctcct tctcaagtcc tttgccactc ttttttgagg tgcaaagtgt taaaaaaatg
    13681 tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct aaacccgttc tgactgttta
    13741 tacctacgat tccttcgccg ccgactgggg gccaggtcca aagatcaaaa aagcttttga
    13801 agccgactgt aactgcgaac tgaaactggt ggcgctggaa gatggcgttt cgcttctcaa
    13861 ccgtctacgg atggaaggca aaaatagtaa agccgatgtg gtgctggggc tggataacaa
    13921 tctgttagac gccgccagta aaaccggact atttgccaaa agcggtgtgg cagcggatgc
    13981 cgttaacgtt cccggcggct ggaataatga cactttcgtc ccgtttgatt acggctattt
    14041 cgccttcgtc tatgacaaga acaaactgaa aaacccgccg caaagcctga aagaattggt
    14101 tgagagcgat cagaactggc gggtgattta tcaggatccg cgtaccagca cgccggggct
    14161 gggtctgctg ttgtggatgc aaaaagtcta tggcgatgac gccccacaag cctggcaaaa
    14221 actggcgaag aaaaccgtca cggtcaccaa aggctggagc gaagcctacg gcctgttttt
    14281 aaaaggtgaa agcaatctgg tactgagtta caccacctct ccggcttatc acattctcga
    14341 agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa ggtcactatc tgcaggtgga
    14401 agtcgccgcc cgcaccgctg ccagcaagca gccggagctg gcgcaaaaat tcctccagtt
    14461 tatggtttct ccggcttttc agaatgcgat cccaaccggt aactggatgt atccggtggc
    14521 aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa ccggccacca cgctggagtt
    14581 cacaccagct gaagtggcgg cacaacgtca ggcatggatt agcgaatggc aacgcgccgt
    14641 cagccgttaa ttcccggctg gttaattcca ggtgtaagcg ccgccacgct ggtggtagcg
    14701 gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc cgcagggtaa ctgggtggca
    14761 gtctggcagg acagctacct gtggcatgtg gtgcgcttct ccttctggca ggcgtttctc
    14821 tcggcgctac tctctgtcgt acccgcgata ttcctcgccc gcgccctcta tcgcaggcgc
    14881 tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga ccttgatcct cccggtgcta
    14941 gttgctgttt tcggcattct tagcgtctat ggtcgtcagg gctggctagc atcgctctgc
    15001 caatcgctcg gtctggagtg gaccttttcg ccctacggcc tgcaaggcat tttgctggcg
    15061 cacgtatttt ttaatctgcc gatggcgagc cgcttattac tccaggcact ggaaaacatt
    15121 cccggcgaac aacgtcagct tgccgcccag cttgggatgc gcggctggca ttttttccgc
    15181 ttcgtcgaat ggccgtggct acggcgacaa atcccgccgg ttgccgcgct tatctttatg
    15241 ctctgtttcg ccagcttcgc caccgtgcta tcgctgggcg gcggtccgca ggcaaccact
    15301 atcgagctgg ctatttatca ggcgctgagt tacgactacg atcctgcccg cgcggcgatg
    15361 ctggcgctgc tccagatggt gtgttgcctt gggctggtgc tgttgagtca gcgactaagt
    15421 aaggccattg cgcccggcac cacgctgctg caaggctggc gcgatccgga cgatcgtctg
    15481 catagccgca tttgcgacac ggtgttaata gtgctggcgc tgctgctgtt gctgccaccg
    15541 ttgctggcgg tgatcgtcga tggggtaaat cgccagttgc cggaagtgct ggcacaaccg
    15601 gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc tggcggcagg tgtattgtgc
    15661 gtagtgctga ccatgatgct gctatggagc agtcgcgaac tgcgggcgcg gcagaaaatg
    15721 ctggcgggtc aggcgctgga gatgagcggc atgttgatcc tcgccatgcc ggggattgtg
    15781 ctggctaccg gcttcttttt actgctcaac aacactatcg gcctgccaca atctgctgac
    15841 ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt atgcgctgaa agtactggaa
    15901 aacccgatgc gcgatatcac cgcccgctac agtatgttgt gtcagtcgct gggcattgaa
    15961 ggctggtcgc gcttaaaagt ggtcgagctg cgcgccctga aacgtccact ggcgcaggcg
    16021 ctggcttttg cctgcgtgct gtcgattggt gattttggcg tagtggcgtt gttcggtaac
    16081 gatgatttcc gtaccctgcc gttttatctc taccagcaaa ttggctccta tcgcagccag
    16141 gacggcgcgg tcaccgcgtt aattctgcta ctactctgtt ttctgctgtt taccgtgatt
    16201 gaaaaactac cggggcgaaa tgttaaaact gactgatatc acctggcttt atcaccattt
    16261 gccgatgcgt tttagcttaa cggtagaacg cggcgagcag gtggcaatcc tcgggccaag
    16321 cggcgcgggt aaaagtaccc tgctgaattt gattgccggt tttctgacgc cagccagcgg
    16381 ttcgctgact atcgatggcg ttgatcacac cacgacgccg ccgtcacgcc gtccggtgtc
    16441 gatgctgttt caggagaaca acctgttcag ccacctgacg gtcgcgcaga acatcgggct
    16501 ggggctaaat ccggggttga aactgaacgc ggcacagcag gagaaaatgc acgcgattgc
    16561 ccgtcagatg gggattgata atttaatggc gcggttaccg ggcgagcttt ccggcggtca
    16621 gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa cagccgattt tattgctcga
    16681 tgaaccgttc tctgcgctcg atccggcgtt acgtcaggag atgctgacgc tggtgagcac
    16741 gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg cacagtgtgg aagatgcggc
    16801 gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc atcgcctggc agggtaagac
    16861 tgaagaattg ttgagcggta aggcgagtgc ttcggcaata ttggggatta cgggttagta
    16921 tgcactgtca gatgcggcgt gaacacctta tccgacctac ggacagtgcg ttttgtaggc
    16981 ctgataagac gcggtcagcg tcgcatgagg caacccgcac aagacaatcc cctaaacccc
    17041 aaccacttta cgcaaaatat cgatatacac cggcatcaac gggtggcgaa ttaacaccac
    17101 cagcgccacc acgccgatgg cagaaatcaa cggcgtcagc cacaacaaac ggccgcgcga
    17161 caaataatga cttaaacgat cggttgcttt accgctgcgc cataaccgcc agcacagcca
    17221 gccaccaacc cacaaaaata ccgccgtcgc cagcagcaac catttaaact cgccgctctg
    17281 cattccggct ggaatatcga tcgccgcgcc cgccagaatc ccaggcagga agtaaaacgg
    17341 tggccacagc aggcagccga taatattcgg cgtaatgaat tttgccaccg gcagatccag
    17401 cattcccgcc accattggca ccagcggacg cgtcgggcca acaaagcgac cgaccagaat
    17461 ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta tcgagtaatg ctttgttttt
    17521 cttcagaaat gaccagcgat gcaacggctt tttaaaacgc cagcccagcc agaaggagat
    17581 ccagtcgccc agcagacaac cgacaatccc tgccagccag gcgtgccaga aacttaactc
    17641 gccgctgcca atcagcgctc ccagccccgc catcagcacc gtaccgggta gaatcaaacc
    17701 gaccagcgcc agcgactcca gaaaggccac caacaccacc gccatcaatg aatacacggt
    17761 agattgggta ataaagtgtt ccagcaatgc ttgcataatg tgcctgtcaa atggacgaag
    17821 cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga ttcgttacta
    17881 attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc
    17941 tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac
    18001 caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct
    18061 ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa
    18121 gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat
    18181 tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacacga ttatccatcg
    18241 gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat
    18301 ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa
    18361 acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaaaccc gtattggcaa
    18421 atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact
    18481 ggtgatacca ttcgcgagcc tccggatgac aaccgtagtg atgaatctct cctggcggga
    18541 acagcaaaat atcacccggt cggcagacaa attctcgtcc ctgatttttc accaccccct
    18601 gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa
    18661 aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg
    18721 agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat
    18781 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact
    18841 ggctcttctc gctaacccaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg
    18901 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc
    18961 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
    19021 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt
    19081 tttggatgga gtgaaacgat ggcgattgca attggcctcg attttggcag tgattctgtg
    19141 cgagctttgg cggtggactg cgccagcggt gaagagatcg ccaccagcgt agagtggtat
    19201 ccccgttggc aaaaagggca attttgtgat gccccgaata accagttccg tcatcatccg
    19261 cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg tgcttgcaga gcttagcgtc
    19321 gaacagcgcg cagctgtggt cgggattggc gttgacagta ccggctcgac gcccgcaccg
    19381 attgatgccg acggtaacgt gctggcgctg cgcccggagt ttgccgaaaa cccgaacgcg
    19441 atgttcgtat tgtggaaaga ccacactgcg gttgaagaag cggaagagat tacccgtttg
    19501 tgccacgcgc cgggcaacgt tgactactcc cgctatattg gcggtattta ttccagcgaa
    19561 tggttctggg caaaaatcct gcatgtgact cgccaggaca gcgccgtggc gcaatctgcc
    19621 gcatcgtgga ttgagctgtg cgactgggtg ccagctctgc tttccggtac cacccgcccg
    19681 caggatattc gtcgcggacg ttgcagcgcc gggcataaat ctctgtggca cgaaagctgg
    19741 ggcggcttgc cgccagccag tttctttgat gagctggacc cgatcctcaa tcgccatttg
    19801 ccttccccgc tgttcactga cacctggact gccgatattc cggtgggcac cttatgcccg
    19861 gaatgggcgc agcgtctcgg cctgcctgaa agcgtggtga tttccggcgg cgcgtttgac
    19921 tgccatatgg gcgcagttgg cgcaggcgca cagcctaacg cactggtaaa agttatcggt
    19981 acttccacct gcgacattct gattgccgac aaacagagcg ttggcgagcg ggcagttaaa
    20041 ggtatttgcg gtcaggttga tggcagcgtg gtgcctggat ttatcggtct ggaagcaggc
    20101 caatcggcgt ttggtgatat ctacgcctgg tttggtcgcg tactcggctg gccgctggaa
    20161 cagcttgccg cccagcatcc ggaactgaaa gcgcaaatca acgccagcca gaaacaactg
    20221 cttccggcgc tgaccgaagc atgggccaaa aatccgtctc tggatcacct gccggtggtg
    20281 ctcgactggt ttaacggtcg tcgcacgcca aacgctaacc aacgcctgaa aggggtgatt
    20341 accgatctta acctcgctac cgacgctccg ctgctgttcg gcggtttgat tgctgccacc
    20401 gcctttggcg cacgcgcaat catggagtgc tttaccgatc aggggatcgc cgtcaataac
    20461 gtgatggcgc tgggcggcat cgcgcggaaa aaccaggtca ttatgcaggc ctgctgcgac
    20521 gtgctgaatc gcccgctgca aattgttgcc tctgaccagt gctgtgcgct cggtgcggcg
    20581 atttttgctg ccgtcgccgc gaaagtgcac gcagacatcc catcagccca gcaaaaaatg
    20641 gccagtgcgg tagagaaaac cctgcaaccg cgcagcgaac aggcacaacg ctttgaacag
    20701 ctttatcgcc actatcagca atgggcgatg agcgccgaac aacactatct tccaacttcc
    20761 gccccggcac aggctgccca ggccgttgcg actctataag gacacgataa tgacgatttt
    20821 tgataattat gaagtgtggt ttgtcattgg cagccagcat ctgtatggcc cggaaaccct
    20881 gcgtcaggtc acccaacatg ccgagcacgt tgttaatgcg ctgaatacgg aagcgaaact
    20941 gccctgcaaa ctggtgttga aaccgctggg caccacgccg gatgaaatca ccgctatttg
    21001 ccgcgacgcg aattacgacg atcgttgcgc tggtctggtg gtgtggctgc acaccttctc
    21061 cccggccaaa atgtggatca acggcctgac catgctcaac aaaccgttgc tgcaattcca
    21121 cacccagttc aacgcggcgc tgccgtggga cagtatcgat atggacttta tgaacctgaa
    21181 ccagactgca catggcggtc gcgagttcgg cttcattggc gcgcgtatgc gtcagcaaca
    21241 tgccgtcgtt accggtcact ggcaggataa acaagcccat gagcgtatcg gctcctggat
    21301 gcgtcaggcg gtctctaaac aggatacccg tcatctgaaa gtctgccgat ttggcgataa
    21361 catgcgtgaa gtggcggtca ccgatggcga taaagttgcc gcacagatca agttcggttt
    21421 ctccgtcaat acctgggcgg ttggcgatct ggtgcaggtg gtgaactcca tcagcgacgg
    21481 cgatgttaac gcgctggtcg atgagtacga aagctgctac accatgacgc ctgccacaca
    21541 aatccacggc gaaaaacgac agaacgtgct ggaagcggcg cgtattgagc tggggatgaa
    21601 gcgtttcctg gaacaaggtg acttccacgc gttcaccacc acctttgaag atttgcacgg
    21661 tctgaaacag cttccaggtc tgcccgtaca gcgtctgatg cagcagggtt acggctttgc
    21721 gggcgaaggc gactggaaaa ccgccgccct gcttcgcatc atgaaggtga tgtcaaccgg
    21781 tctgcagggc ggcacctcct ttatggagga ctacacctat cacttcgaga aaggtaatga
    21841 cctggtgttc ggctcccata tgctggaagt ctgcccgtcg attgccgtag aagagaaacc
    21901 gatcctcgac gttcagcatc tcggtattgg tggtaaggac gatcctgccc gactgatctt
    21961 caatacccaa accggtccag cgattgtcgc cagcctgatt gatctcggcg atcgttaccg
    22021 tctgctggtt aactgtatcg acacggtgaa aacaccgcac tccctgccga aactgccggt
    22081 ggcgaatgca ctgtggaaag cgcaaccgga tctgccaact gcttccgaag cgtggatcct
    22141 cgctggtggc gcgcaccata ccgtcttcag ccatgcgctg aacctcaacg atatgcgcca
    22201 gttcgccgag atgcacgaca ttgaaatcac ggtgattgat aacgacaccc gcctgccagc
    22261 gtttaaagac gcactgcgct ggaacgaagt gtattacgga tttcgtcgct aagtagccac
    22321 atcaggtatg taacgcctga tgcgacgctg acgcgtctta tctggcctac acgctgcgat
    22381 tttgtaggcc ggataagcaa agcgcatccg gcattcaacg cctgatgcga cgctggcgcg
    22441 tcttatcagg cctacgcgct gcgattttgt agaccggata agcgaagcgc atccggcacg
    22501 aaggagtcaa catgttagaa gatctcaaac gccaggtatt agaagccaac ctggcgctgc
    22561 caaaacacaa cctggtcacg ctcacatggg gcaacgtcag cgccgttgat cgcgagcgcg
    22621 gcgtctttgt gatcaaacct tccggcgtcg attacagcgt catgaccgct gacgatatgg
    22681 tcgtggttag catcgaaacc ggtgaagtgg ttgaaggtac gaaaaagccc tcctccgaca
    22741 cgccaactca ccggctgctc tatcaggcat tcccctccat tggcggcatt gtgcatacgc
    22801 actcgcgcca cgccaccatc tgggcgcagg cgggtcagtc gattccagca accggcacca
    22861 cccacgccga ctatttctac ggcaccattc cctgcacccg caaaatgacc gacgcagaaa
    22921 tcaacggcga atatgagtgg gaaaccggta acgtcatcgt agaaaccttt gaaaaacagg
    22981 gtatcgatgc agcgcaaatg cccggcgttc tggtccattc ccacggcccg tttgcatggg
    23041 gcaaaaatgc cgaagatgcg gtgcataacg ccatcatgct ggaagaggtc gcttatatgg
    23101 ggatattctg ccgtcagtta gcgccgcagt taccggatat gcagcaaacg ctgctggata
    23161 aacactatct gcgtaagcat ggcgcgaagg catattatgg gcagtaatga ctgtataaaa
    23221 ccacagccaa tcaaacgaaa ccaggctata ctcaagcctg gttttttgat ggattttcag
    23281 cgtggcgcag gcaggtttta tcttaacccg acactggcgg gacaccccgc aagggacaga
    23341 agtctccttc tggctggcga cggacaacgg gccgttgcag gttacgcttg caccgcaaga
    23401 gtccgtggcg tttattcccg ccgatcaggt tccccgcgct cagcatattt tgcagggtga
    23461 acaaggcttt cgtctgacac cgctggcgtt aaaggatttt caccgccagc cggtgtatgg
    23521 cctttactgt cgcgcccatc gccaattgat gaattacgaa aagcgcctgc gtgaaggtgg
    23581 cgttaccgtc tacgaggccg atgtgcgccc gccagaacgc tatctgatgg agcggtttat
    23641 cacctcaccg gtgtgggtcg agggtgatat gcacaatggc actatcgtta atgcccgtct
    23701 gaaaccgcat cccgactatc gtccgccgct caagtgggtt tctatagata ttgaaaccac
    23761 ccgccacggt gagctgtact gcatcggcct ggaaggctgc gggcagcgca tcgtttatat
    23821 gctggggccg gagaatggcg acgcctcctc gcttgatttc gaactggaat acgtcgccag
    23881 ccgcccgctg ctgctggaaa aactcaacgc ctggtttgca aaccacgatc ccgatgtgat
    23941 catcggctgg aacgtggtgc agttcgatct gcgaatgctg caaaaacatg ccgagcgtta
    24001 ccgtcttccg ctgcgtcttg ggcgcgataa taacgagctg gagtggcgcg agcacggctt
    24061 taaaaacggc gtcttttttg cccaggctaa aggtcggcta attatcgacg gtatcgaggc
    24121 gctgaaatcc gcgttctgga atttctcttc attctcgctg gaaactgtcg ctcaggagct
    24181 attaggcgaa ggaaaatcta tcgataaccc gtgggatcga atggacgaaa ttgaccgccg
    24241 tttcgccgaa gataaacctg cgctggcaac ttataacctg aaagattgcg agctggtgac
    24301 gcagatcttc cacaaaactg aaatcatgcc atttttactc gaacgagcaa cggtgaacgg
    24361 cctgccggtg gatcggcacg gcggttcggt ggcagcgttt ggtcatctct attttccgcg
    24421 aatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa gtgccgccgc acgccagccc
    24481 tggcggctac gtgatggatt cacggccagg gctttatgat tcagtgctgg tgctggacta
    24541 taaaagcctg tacccgtcga tcatccgcac ctttctgatt gatcccgtcg ggctggttga
    24601 aggcatggcg caacctgatc cagagcacag taccgaaggt tttctcgatg cctggttctc
    24661 acgggagaaa cattgcctgc cggagattgt gactaacatc tggcacgggc gcgatgaagc
    24721 caaacgtcag ggtaataagc cgctttcgca ggcgctgaag atcattatga atgcctttta
    24781 tggcgtgctc ggcaccaccg cctgccgctt cttcgatccg cgcctggcat cgtcgatcac
    24841 catgcgtggt catcagatca tgcggcagac caaagcattg attgaagcgc agggctacga
    24901 cgttatctac ggagataccg actcgacttt tgtctggcta aaaggtgcac attcggaaga
    24961 agaggcggcg aaaatcggtc gtgcactggt gcagcacgtt aacgcctggt gggcggaaac
    25021 gctgcaaaaa caacggctga ccagcgcatt agaactggag tatgaaactc atttctgccg
    25081 ttttcttatg ccaaccattc gcggagctga taccggcagt aaaaagcgct acgccgggct
    25141 gattcagaag ggcgataagc agcggatggt gtttaaaggg ctggaaaccg tgcgcaccga
    25201 ctggacgccg ctggcccagc agtttcagca ggagctgtac ctgcgtatct tccgcaacga
    25261 gccatatcag gagtacgtcc gccaaaccat cgacaaactg atggcgggtg aactggatgc
    25321 gcggctggtt taccgtaaac gccttcgccg tccgctgagc gaatatcagc gtaatgttcc
    25381 gcctcatgta cgcgccgctc gccttgccga tgaagaaaat caaaagcgcg gtcgcccctt
    25441 gcaatatcag aatcgcggca ccattaagta cgtatggacc accaacggcc cggagccgct
    25501 ggactaccaa cgttcaccac tggattacga acactatctg acccgccagc tacaacccgt
    25561 ggcggaggga atactccctt ttattgagga taattttgct acacttatga ccgggcaact
    25621 tgggctattt tgagcaaaaa aaagagttcg ccagatacca ttttgatgcg tgacgaatgc
    25681 tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca ccactacctc
    25741 ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg aacacatatg
    25801 ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg acttggaacc
    25861 gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg tgaaaaccgt
    25921 ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg tgataccatt
    25981 accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa tggcttgctg
    26041 acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg tgaagttttc
    26101 cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg gcagattgac
    26161 cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga acagttccgt
    26221 atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca gctcaacatc
    26281 gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga agtgggttta
    26341 gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg cgctgctgaa
    26401 cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt ggctggtaga aatgctgcgc
    26461 cgtttcaacc tgcgctttgc tctgtttgat gatgagcgtt atgccgaagc tcagcacgat
    26521 gcttacaacc cgtttgacac cgaacagctg gtgatttgct cgctggattt tgcccgtcgt
    26581 agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt ggtcgatgaa
    26641 gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc cattgaacaa
    26701 ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca gctggggatg
    26761 gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga ttttgcgcag
    26821 ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat gctgctggca
    26881 ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg cgagcaggat
    26941 atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag cgcccgtcag
    27001 gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt ccgtaacacg
    27061 cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct gccgctaccg
    27121 acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa aagtgcggaa
    27181 gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga aggtgataac
    27241 gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct gaccagccat
    27301 cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca actggagcag
    27361 gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat gtcgattatc
    27421 gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca ggtactgctg
    27481 tgctcagaaa tcggttctga aggacgtaac tttcagttcg ccagccacat ggtgatgttt
    27541 gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga tcgtatcggc
    27601 caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca gtcggtgctg
    27661 gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac cggacgcact
    27721 atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccgga tcaaaccgaa
    27781 ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa agcacagctg
    27841 gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa agcccaggca
    27901 ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt cgccatgaac
    27961 ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat cgtgctgacg
    28021 ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg catcaccatc
    28081 acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac ctgggagcat
    28141 ccgctgatcc gcaacggtct ggatctgatc ctttctggcg ataccggtag cagcacgatt
    28201 tcactgttaa aaaacaaagc gttgccagta ggtacgctgt tggtggaact gatttatgtg
    28261 gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc gacgccggta
    28321 cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt tgaaaccttt
    28381 aaccgccagc ttaacgcggt taaccgtcac accggcagca aactggttaa cgccgtgcag
    28441 caggatgttc acgctatcct tcaactgggt gaagcgcaga tcgagaaatc tgcccgtgca
    28501 ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt ctgccgagct gtctcgtctg
    28561 gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg aactgaccgc cattgagagc
    28621 aaccgtcagc aggtaatgga aagcctggat caggcaggtt ggcgtctgga tgccctgcgt
    28681 ttgatcgttg taacgcatca gtaacggagt cgaaaatggg gatggaaaac tacaatccac
    28741 cgcaggaacc ctggttggtt atcctgtatc aggatgacca tattatggtg gtcaacaagc
    28801 cgagcggttt gttgtcagtg ccgggtcgtc tggaagagca caaagacagc gtgatgacgc
    28861 gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg gctaccagcg
    28921 gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc cagttccgcg
    28981 agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc cccgcagaag
    29041 gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag aaagtctgtt
    29101 acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg gcggataaca
    29161 cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt gtgcatatgc
    29221 tggcgctggg gcatccgatt ctcggcgatc gtttttatgc atcaccagaa gcgagagcga
    29281 tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg gcgtatggca
    29341 atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc agttcggtag
    29401 gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga tgcgacgcgg
    29461 gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc tgctttatca
    29521 gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc atctcaggcg
    29581 gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta cggtaggcac
    29641 gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag gcatcttcca
    29701 gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc tgataaccgc
    29761 cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg gcgcgggaga
    29821 tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc agtgaaccat
    29881 cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca aaacgaccaa
    29941 agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct aattttccca
    30001 cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt cggtccatca
    30061 actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg gttaaatgcc
    30121 ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg ttggcgaacc
    30181 acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg cctaacacta
    30241 cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt tttccccaat
    30301 actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac aatataaagg
    30361 tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat ttgcattatc
    30421 atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag gattaacact
    30481 agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac gttaccgatg
    30541 atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc caccatgatt
    30601 gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca gtgcatgttg
    30661 ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt acccgtgact
    30721 atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac tggcagcgtg
    30781 gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca tcaaaaagag
    30841 gcactaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt ccattacgac
    30901 gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac caaagatacc
    30961 aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa agcggacctg
    31021 atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt tacctcctgt
    31081 ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga ccgcgaagaa
    31141 caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat cttttatagc
    31201 ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat cccgaacgcc
    31261 aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa catcgcgcca
    31321 aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat catgtgggag
    31381 aacgaattcc gctacctctc ccaggctggc gctggcttga tggaactgga ctatctgcct
    31441 tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg ttggttattc
    31501 tactggaacc actccggggt catggatcag gtgtggcgtt tcaacgtcga ctacaccaag
    31561 gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag tactgacggc
    31621 tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc caccgtttca
    31681 accaagcagt tccaggtttt cagcgaacag aacaccagta gctactcggc agagccgcag
    31741 ttagacgtta attactacca gaatgatgtt ggtccgtttg atacgcgtat ttacggccag
    31801 gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt tcacctggaa
    31861 ccgaccatca atttgcctct ctctaataac tggggcagca tcaataccga agcgaagttt
    31921 ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa cacgaccaag
    31981 ctggacgaat ccgttaaccg cgtaatgccg caattcaaag ttgacggcaa aatggtcttt
    32041 gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc gcgcgcgcag
    32101 tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc gtctctgctg
    32161 caatctgact actctggcct gttccgggac cggacttacg gcggtcttga ccgtattgcc
    32221 tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc tgccgttgaa
    32281 cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac tggcgatgac
    32341 aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg cgacacctac
    32401 tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac acgtctggat
    32461 aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg tctggtacag
    32521 ctgaattacc attacgccag cccggaatat attcaggcta cgctgcctaa gtactattcc
    32581 actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag ctggccaatt
    32641 gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa caagcaagcc
    32701 gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt cggttacgag
    32761 cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa cgcaatcggc
    32821 tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca agagatgctg
    32881 cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc acgtaatccg
    32941 cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc ttctcggtat
    33001 cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag tcgcagccgt
    33061 cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt cggtaaaact
    33121 gaacgctgcg caggcaaggc agcagcttcc tgatgacacg acgctgcgcc accaaatcat
    33181 ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg gagtgaaaat
    33241 ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca acatgacgct
    33301 ggatcagatg cgcggccgtc tggcttacga tggtctgaac tacaacactt atcgtaacca
    33361 gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc gtcgcatcac
    33421 catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa acgacgccag
    33481 cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga cctctgatca
    33541 ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta acggcgctga
    33601 tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg gcggccagat
    33661 ggggtggggc cgtattcagg agttgccggg gatcttcgcc caggcattaa gcaccgcgaa
    33721 gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc tgaaagttaa
    33781 cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc gccatattct
    33841 gctgaaaccg tcgccgatca tgactgacga acaggcccgc gtgaaactgg agcaaattgc
    33901 tgctgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt tctcgcagga
    33961 tccaggttct gctaaccagg gcggtgatct cggctgggct acaccagata ttttcgatcc
    34021 agccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac cggttcactc
    34081 ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata aaaccgacgc
    34141 tgcgcagaaa gatcgtgcat accgcatgct gatgaaccgt aagttctcgg aagaagcagc
    34201 aagctggatg caggaacaac gtgccagcgc ctacgttaaa atcctgagca actaatggtt
    34261 aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc ggacttagtt
    34321 gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga tgccactttc
    34381 cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta ttcccccaac
    34441 tcccctgcac aaccgcaaac tacgggcaca ttaacgctac ttcctgtcgc gctacgtgaa
    34501 tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga gacgctggcg
    34561 cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg ccccgtgcat
    34621 aaaggcgtca ttaacgacgc aagcattccg tttaccggtc ataccgagtt tttcgaagag
    34681 cgttcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg cgtggcgctg
    34741 gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc acttttgcac
    34801 gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc cgaaccgcgc
    34861 attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg tacggaagag
    34921 atagacacca ttattccggt gctcgacgag ctgcgggcgc aggggatgaa actcaacggg
    34981 ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga cgccgtgctg
    35041 gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg gcgcggtgtg
    35101 aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac cgcgcttgaa
    35161 ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa tctcgccatc
    35221 aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta gcccgtaaac
    35281 gcttcgggca aaactttctc aacgatcact tcgtgatcga cagcattgtt tctgccatta
    35341 acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tctggcggca ttgaccgagc
    35401 cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat ctggcggcac
    35461 gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag gatgcgatga
    35521 cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt ttcggcaacc
    35581 tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact gatgccattg
    35641 ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca ggaccgaaca
    35701 gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg atcccggtac
    35761 tggaagttcc gccgtcagcc tttacaccac cacccaaagt ggattccgcc gtcgtgcgcc
    35821 tagtccctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg agccgcatca
    35881 ccaccgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc aacctgttta
    35941 gcgtcgaggt gttaaccgga atggggatcg acccggcgat gcgagcggaa aatatctctg
    36001 tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg caggagagtt
    36061 aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca ttgaggcaca
    36121 atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac gcaatctggg
    36181 gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca atggccgtga
    36241 aaccgaagtc catggcgaag gagtggttgg cgtccagcca cttatcgcgc ctggcgaaga
    36301 gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc agggtcacta
    36361 cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat tccgactcgc
    36421 cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg acgttcatgg
    36481 ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccccg ggaaagatac
    36541 cctctggctg acgggcgatc tggtcgcgcg cggcccgggt tcgctggatg ttctgcgcta
    36601 tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc tgcatctgct
    36661 ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac cgctgctgga
    36721 agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctctgc tgcaaatcga
    36781 cgaagagaaa aagctggtga tggcccacgc agggatcacg ccgcagtggg atctgcagac
    36841 cgccaaagag tgcgcgcgcg atgtagaagc ggtgctgtcg agtgactcct atcccttctt
    36901 tcttgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc gggggctggg
    36961 aagactgcgt tttatcacca acgcctttac ccgtatgcgt ttttgcttcc cgaacggtca
    37021 actggatatg tacagcaaag aatcgccgga agaagcccct gtcccactga aaccgtggtt
    37081 tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact gggcatcgct
    37141 ggaaggcaaa ggtacgccgg aaggtattta cgcgctggat accggctgct gctggggtgg
    37201 gtcattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt cgaaccggca
    37261 taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatacaggaa ggccggataa
    37321 gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaactt accgccgctc
    37381 cagaatctca aagcaatagc tgtgagagtt ctgcgcatcg gcgtcgtgga attcgctgaa
    37441 taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt ccacttctgc
    37501 gtcgatatgc gtcagataca gtttttgcgc ttttggcagg aactgctcat aaacgcgacc
    37561 gccgccaatc accatgattt ctggtacgtc accacacgcc gcaatggctt catccaccga
    37621 tttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa tatttttgcg
    37681 tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca cgggtttatt
    37741 taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca tggcgttttc
    37801 catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg agatttcccg
    37861 ataaaaaaat tgtcgccact atacgtaaag cgtaaaccgt cgtcgactgg tgcgaggatg
    37921 atgttgagga aaattttata ttctgctggc gagtccacgc tctccccctg gactcgccgc
    37981 attacaatga aacaaaaaca aacagttagc tgtaaagtgt gatttacgtc actctttatt
    38041 aagatgaggg tttcgtttcc ggttcatccg ccatgttgcc ggtatgttta ccttcttctg
    38101 ttccctgcca gccatgtcgt tgaattaatg acagatgttc gcggtcctcg gtgatgatct
    38161 cacttaacat cgcgctggtg cgtttataga ccgccgcgcg ggctttggtg tcattctcaa
    38221 ccattgccat ctcttccacc atctgaatat taaagcggcg gaacacatcg gcacgttctc
    38281 gcgcttcata cggccccaga cctaaacttt ccagcgccag acgcccggtt ttcagcgcac
    38341 cttcaaaggt ttcacgctcc ggcttttcaa ggcctgcctg acgcaaacga atgtagtgat
    38401 cgacatcgcg ggcgcgggca ataatctgca aatgcgggaa atgttctttc accatctccg
    38461 tcagttgcag gttggtttgc ggatcgtcaa tagcgttaat cagcacttcc gctttcgccg
    38521 ctccggcaga ttccagtaaa tccatccgcg tggcatcgcc ataaaacact ttcataccaa
    38581 atttacgcag cgtttcgata tggtccggat cgtgatcgag caccaccatt ttcaccccgc
    38641 tggagagcag taaacgtccg gtaatctgtc caaaacgacc gaatccggcg ataatcacgc
    38701 gcggctgttc ttcatcgatc tcatcggctt cacgcgcctc ctccgtagaa gattgctcaa
    38761 ggcgattgag gatcaccagc agaatcggcg ttgctgccat cgacagcgcc accgccaggg
    38821 tcagcgattt cgcccactcc ggctccagca cattcgccat ctgcgccgca ccaaatacca
    38881 caaaggcaaa ctcactgccc tgccctaaca acaccgcaaa ccagcgacgc tgtttatttg
    38941 gcacctgcaa cggtcgggca atcagccaca gcatggcgat tttgatgatg aggaaaccga
    39001 gcagcaaaat gacaatgcgc aatgggtttt caatcagcgt gccaaagtct atcgacatgc
    39061 caacaccgat gaaaaacagc cccaacagca aacctttaaa cggttcgata tcgctttcca
    39121 acgcatgacg gtattccgag cttgccagca gtacgcccgc cagaaacgcg cccatcgcca
    39181 tcgacaagcc gacctcttcc agcagcaaac caaagccaaa cacaaggaat aacgccacga
    39241 cactaaacac ttcccgcaag ccagagcggg caacaaaacg cagcgccgga cgcgtaacat
    39301 agcgccccag caataccacc agtaccagcg cacccgccac ttttaacgcc gagagagcaa
    39361 atgcgcccat cgtcgtcgag gcactgctcg ccgccagtag cgggatcatc gccaccagcg
    39421 ggattgccgc gatatcctgg aacagcagca ccgcaaaggc actgcggccc atttgcgtca
    39481 ccatcagatt gcgttcattc atcgcctgca tggcaatcgc cgttgatgag agcgccagcg
    39541 tcataccgat caattccgcg acctgccagc gcagcccaag taacatacag aacagcccca
    39601 gcaggccacc gcaaatcacc atctgcaatg cgccaccgcc gaacactgcc gcacgcagct
    39661 tccacagcct ttgtggatcg agttcgaggc gataataaac agcatcagca ccaccccaat
    39721 ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc acgggccaat
    39781 aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta ccgcaatggg
    39841 tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat gactatccat
    39901 gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc ccttcgaggg
    39961 tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag ttcagcccgc
    40021 agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga tgcgcaccaa
    40081 tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt ttgccatgca
    40141 gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg atccaaagtt
    40201 taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc agatcggcgc
    40261 gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag agttgataaa
    40321 gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt ttattcgcat
    40381 gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt agcccgcgtg
    40441 tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta tagtcaataa
    40501 tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc cgcggatcgt
    40561 catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg ctacggaaat
    40621 caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga taccgtaatt
    40681 attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac ttaaggacat
    40741 aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag gatagagcca
    40801 ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca tcactaccag
    40861 ccagatcccc atatccggga ttagccccaa aaccgccagc gccagcgtca tcatggcaaa
    40921 gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg gcggaataca
    40981 gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt tgccaacccc
    41041 caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca ttgggatcac
    41101 ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc gattaaacag
    41161 atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt cgccaaacag
    41221 ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc agcggggaga
    41281 ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg cgctacccag
    41341 catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat cggcgcaggt
    41401 cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct gacgggtact
    41461 ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga caatgccgat
    41521 gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg ccaccgatat
    41581 cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt tatcggagat
    41641 atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc ccagcaagcc
    41701 aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca cgccaatcat
    41761 taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa tgcggcggtg
    41821 aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt attcctgttt
    41881 ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg taacggaact
    41941 ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc agccccagaa
    42001 tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg ccgtcatcct
    42061 gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc tgtttatcag
    42121 catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta ttgacgccca
    42181 gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca tgccatcctt
    42241 gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc gcatcagttc
    42301 cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca gttccgggta
    42361 gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca tgcgcatatc
    42421 gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct tactgaaatc
    42481 gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg caatcgccag
    42541 atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat caccagcaat
    42601 caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg gcactacgtg
    42661 agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg gatgatgttt
    42721 gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat gcagaccaca
    42781 aaccagcccc agcgacaggg tattttcact ggtataaagg aagccgccgc ccatcaggcc
    42841 atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca actgaaaacg
    42901 gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg ccacatccgt
    42961 cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca ccccatcagc
    43021 aaggatcacc gttttcgctt caatcacatc gccatcggct tctacaccga cgactttgcc
    43081 atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc tcgcttcttc
    43141 ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag aacgctggga
    43201 tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt ccgtcataaa
    43261 cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga aacctggaat
    43321 aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg cacctgcgga
    43381 attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca gtgcggcaac
    43441 cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg acataagagc
    43501 tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg cgctaaagct
    43561 gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc cgcgtactgg
    43621 aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc gttagcgcca
    43681 accatgtgct ggatctgccc ggagatcccc accgccaggt acagttcagg tttcagcatc
    43741 aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc tgccaccgga
    43801 cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag cgcaatgttc
    43861 tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc gagatcgacg
    43921 ctgttgctct ggcgcgcctg gctgagccgc atcgaacgtg ccgctgctga tggtcagtac
    43981 cgcatacggc gtggcaatgc gttcttcgcc aatcgccaga ccgccgtaaa ccatgtgttt
    44041 cactgttgct ttaccgtcct gtacgctgac ggtgctggca tcgttagaca ccgccgcttt
    44101 aaggcgataa cccagttttg ccgccagtaa tttgccgcga cgggtgtttg gcaacagcac
    44161 cagaccgtca gcgccgtgct ggcgaatagt gtcagccatg acaccggcgt aatcttcgat
    44221 catccgatcg tccggtttgc cgtttaattt ccagacatga ttagcgccga gctggattgc
    44281 ctgtgcgccg tcggcatcat tgaggacaaa ggcattgatt tgattagcta aagcctgcgc
    44341 accgttcatc agttccggca gacgagaggg ggtatcgctg aatacccaga cttgagaaaa
    44401 cgtgttcata gcatcccctg taattaaatg actttgcgaa gattttcagc aaatgcggcg
    44461 atctgttctt cgccgtcgcc ttcaatcacg atgcgctgac gctcgcgctg tttcggcgcg
    44521 gcaacctgtt gttcagacca ggcgtccacg gcgttaaaac caatatccgc cgccgaccat
    44581 acctggactg gctttttcgc cgcgccgaga atggctttca tcgaaggaat ttgtggggag
    44641 ttgatatcag tggaaacagc aacaaccgca ggcagcggaa tgcttaaggt ttcggtttca
    44701 tcttccagtt cgcgctcaac ggtgagggta tctgccgtca gggagataat tttgctgacg
    44761 ccgttaactg ccggaatatt gaggatttcg cccaccagca gaccaacctg ctgggcataa
    44821 aggtcggaag aaccatcgcc acagaggatc agatcaaagc ctgctttctg ggcggctgca
    44881 gccagtgcgc tcgccgtttg ttgcggcagt gcctgctcga actggtcatc aatcaccaca
    44941 atcagttcat ccgggccgcg cgatagcaca tctttacgcc ctttggcgtt ggtcagggct
    45001 ttaccgccca cacttaaggc tgtcacctgc gcctctgctg cctgttgctt tagctggcaa
    45061 gccgcttcaa tagcgttgag atcgtattgg cttattttgg catcggcttt gctgaagtct
    45121 aatgaaccat cagcattatt gaccgcaata tcctgttcat caggcacgca cttatagcaa
    45181 gtaataatct tcattgcatc tccagaaatc atgaaggaaa cgtcgttcat atcggtggtg
    45241 ttaattacag atatcacgcc ctttaatttg gaaataaaac tttaatcacc aatattgaaa
    45301 atgtcacacg cagataaatc caactttcaa tattgttaag ttcctcacca atattgaaaa
    45361 catggcgtag caaaaagaaa ttttcaatat tgttttatag atcaccaata ttgaaagtca
    45421 gatgctattt ttttacccac aaaagaacat aacaaactga ttaataacca aaattaacgg
    45481 aaaacgtcct ctattcaccc gaaacaaaaa tgtgacaaca atcacagaat acagcttatt
    45541 gaatacccat tatgagttag ccattaacgc gtccacgagg ttaataataa ttatattaaa
    45601 tgttaacaaa aataaaacaa acgggaacgc aaaataaata ttcgttttca cagtggaatt
    45661 aactcatgaa gaatgaaaag agaaaaacgg gaatagaacc gaaggttttc tttccgccat
    45721 taataatcgt cggcatactt tgttggctta cagtcagaga tctggatgcg gcgaatgtcg
    45781 ttattaatgc tgtattcagt tacgtcacca atgtatgggg atggg
//
LOCUS       DBKEKI010000019        45272 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.019, whole genome shotgun sequence.
ACCESSION   DBKEKI010000019 DBKEKI010000000
VERSION     DBKEKI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.019"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..550)
                     /locus_tag="ACUQPX_000991"
     CDS             complement(<1..550)
                     /locus_tag="ACUQPX_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HHV9055766.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDT"
     gene            755..1621
                     /gene="yghU"
                     /locus_tag="ACUQPX_000992"
     CDS             755..1621
                     /gene="yghU"
                     /locus_tag="ACUQPX_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HHV9055767.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(1744..1992)
                     /gene="hybG"
                     /locus_tag="ACUQPX_000993"
     CDS             complement(1744..1992)
                     /gene="hybG"
                     /locus_tag="ACUQPX_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HHV9055768.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(2126..2467)
                     /gene="hypA"
                     /locus_tag="ACUQPX_000994"
     CDS             complement(2126..2467)
                     /gene="hypA"
                     /locus_tag="ACUQPX_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HHV9055769.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCRGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(2460..2948)
                     /gene="hybE"
                     /locus_tag="ACUQPX_000995"
     CDS             complement(2460..2948)
                     /gene="hybE"
                     /locus_tag="ACUQPX_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HHV9055770.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMYDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(2941..3435)
                     /locus_tag="ACUQPX_000996"
     CDS             complement(2941..3435)
                     /locus_tag="ACUQPX_000996"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HHV9055771.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(3435..5138)
                     /gene="hybC"
                     /locus_tag="ACUQPX_000997"
     CDS             complement(3435..5138)
                     /gene="hybC"
                     /locus_tag="ACUQPX_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HHV9055772.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(5135..6313)
                     /gene="hybB"
                     /locus_tag="ACUQPX_000998"
     CDS             complement(5135..6313)
                     /gene="hybB"
                     /locus_tag="ACUQPX_000998"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HHV9055773.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(6303..7289)
                     /gene="hybA"
                     /locus_tag="ACUQPX_000999"
     CDS             complement(6303..7289)
                     /gene="hybA"
                     /locus_tag="ACUQPX_000999"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HHV9055774.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWLNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(7292..8410)
                     /gene="hybO"
                     /locus_tag="ACUQPX_001000"
     CDS             complement(7292..8410)
                     /gene="hybO"
                     /locus_tag="ACUQPX_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HHV9055775.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(8599..8886)
                     /gene="yghW"
                     /locus_tag="ACUQPX_001001"
                     /pseudo
     CDS             complement(8599..8886)
                     /gene="yghW"
                     /locus_tag="ACUQPX_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
     gene            complement(9005..9892)
                     /gene="yghX"
                     /locus_tag="ACUQPX_001002"
     CDS             complement(9005..9892)
                     /gene="yghX"
                     /locus_tag="ACUQPX_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HHV9055776.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMMA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            10049..11089
                     /gene="gpr"
                     /locus_tag="ACUQPX_001003"
     CDS             10049..11089
                     /gene="gpr"
                     /locus_tag="ACUQPX_001003"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HHV9055777.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(11129..11623)
                     /locus_tag="ACUQPX_001004"
     CDS             complement(11129..11623)
                     /locus_tag="ACUQPX_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HHV9055778.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            11814..12698
                     /gene="yghA"
                     /locus_tag="ACUQPX_001005"
     CDS             11814..12698
                     /gene="yghA"
                     /locus_tag="ACUQPX_001005"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HHV9055779.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(12970..13395)
                     /gene="exbD"
                     /locus_tag="ACUQPX_001006"
     CDS             complement(12970..13395)
                     /gene="exbD"
                     /locus_tag="ACUQPX_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HHV9055780.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(13402..14136)
                     /gene="exbB"
                     /locus_tag="ACUQPX_001007"
     CDS             complement(13402..14136)
                     /gene="exbB"
                     /locus_tag="ACUQPX_001007"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HHV9055781.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            14388..15575
                     /gene="metC"
                     /locus_tag="ACUQPX_001008"
     CDS             14388..15575
                     /gene="metC"
                     /locus_tag="ACUQPX_001008"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HHV9055782.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLYIGLEDVDDLIADLDAGFA
                     RIV"
     gene            15715..16374
                     /gene="yghB"
                     /locus_tag="ACUQPX_001009"
     CDS             15715..16374
                     /gene="yghB"
                     /locus_tag="ACUQPX_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HHV9055783.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(16414..17313)
                     /gene="yqhC"
                     /locus_tag="ACUQPX_001010"
     CDS             complement(16414..17313)
                     /gene="yqhC"
                     /locus_tag="ACUQPX_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HHV9055784.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            17507..18670
                     /gene="yqhD"
                     /locus_tag="ACUQPX_001011"
     CDS             17507..18670
                     /gene="yqhD"
                     /locus_tag="ACUQPX_001011"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HHV9055785.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHFSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            18775..19602
                     /gene="dkgA"
                     /locus_tag="ACUQPX_001012"
     CDS             18775..19602
                     /gene="dkgA"
                     /locus_tag="ACUQPX_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HHV9055786.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPETDQFGG"
     gene            19802..20728
                     /locus_tag="ACUQPX_001013"
     CDS             19802..20728
                     /locus_tag="ACUQPX_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HHV9055787.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            20779..21036
                     /gene="yqhH"
                     /locus_tag="ACUQPX_001014"
     CDS             20779..21036
                     /gene="yqhH"
                     /locus_tag="ACUQPX_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HHV9055788.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYVAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(21079..23298)
                     /locus_tag="ACUQPX_001015"
     CDS             complement(21079..23298)
                     /locus_tag="ACUQPX_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HHV9055789.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(23409..24821)
                     /gene="ftsP"
                     /locus_tag="ACUQPX_001016"
     CDS             complement(23409..24821)
                     /gene="ftsP"
                     /locus_tag="ACUQPX_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HHV9055790.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGCPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(24896..25633)
                     /gene="plsC"
                     /locus_tag="ACUQPX_001017"
                     /gene_synonym="parF"
     CDS             complement(24896..25633)
                     /gene="plsC"
                     /locus_tag="ACUQPX_001017"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HHV9055791.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(25867..28125)
                     /gene="parC"
                     /locus_tag="ACUQPX_001018"
     CDS             complement(25867..28125)
                     /gene="parC"
                     /locus_tag="ACUQPX_001018"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HHV9055792.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(28671..29153)
                     /locus_tag="ACUQPX_001019"
     CDS             complement(28671..29153)
                     /locus_tag="ACUQPX_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HHV9055793.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(29206..29598)
                     /gene="ygiW"
                     /locus_tag="ACUQPX_001020"
     CDS             complement(29206..29598)
                     /gene="ygiW"
                     /locus_tag="ACUQPX_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HHV9055794.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVENAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            29750..30409
                     /gene="qseB"
                     /locus_tag="ACUQPX_001021"
     CDS             29750..30409
                     /gene="qseB"
                     /locus_tag="ACUQPX_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HHV9055795.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            30406..31755
                     /gene="qseC"
                     /locus_tag="ACUQPX_001022"
     CDS             30406..31755
                     /gene="qseC"
                     /locus_tag="ACUQPX_001022"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HHV9055796.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(31801..32133)
                     /locus_tag="ACUQPX_001023"
     CDS             complement(31801..32133)
                     /locus_tag="ACUQPX_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HHV9055797.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            32452..33033
                     /gene="mdaB"
                     /locus_tag="ACUQPX_001024"
     CDS             32452..33033
                     /gene="mdaB"
                     /locus_tag="ACUQPX_001024"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HHV9055798.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            33064..33378
                     /locus_tag="ACUQPX_001025"
     CDS             33064..33378
                     /locus_tag="ACUQPX_001025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HHV9055799.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHSYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(33426..35318)
                     /gene="parE"
                     /locus_tag="ACUQPX_001026"
     CDS             complement(33426..35318)
                     /gene="parE"
                     /locus_tag="ACUQPX_001026"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HHV9055800.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(35347..35928)
                     /gene="yqiA"
                     /locus_tag="ACUQPX_001027"
     CDS             complement(35347..35928)
                     /gene="yqiA"
                     /locus_tag="ACUQPX_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HHV9055801.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(35928..36755)
                     /gene="cpdA"
                     /locus_tag="ACUQPX_001028"
     CDS             complement(35928..36755)
                     /gene="cpdA"
                     /locus_tag="ACUQPX_001028"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HHV9055802.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(36780..37202)
                     /locus_tag="ACUQPX_001029"
     CDS             complement(36780..37202)
                     /locus_tag="ACUQPX_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HHV9055803.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(37203..37832)
                     /gene="nudF"
                     /locus_tag="ACUQPX_001030"
     CDS             complement(37203..37832)
                     /gene="nudF"
                     /locus_tag="ACUQPX_001030"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HHV9055804.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            38037..39518
                     /gene="tolC"
                     /locus_tag="ACUQPX_001031"
     CDS             38037..39518
                     /gene="tolC"
                     /locus_tag="ACUQPX_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HHV9055805.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQMKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            39666..40337
                     /locus_tag="ACUQPX_001032"
     CDS             39666..40337
                     /locus_tag="ACUQPX_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HHV9055806.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGSGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            40343..41503
                     /locus_tag="ACUQPX_001033"
     CDS             40343..41503
                     /locus_tag="ACUQPX_001033"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HHV9055807.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(41541..42329)
                     /gene="ygiD"
                     /locus_tag="ACUQPX_001034"
     CDS             complement(41541..42329)
                     /gene="ygiD"
                     /locus_tag="ACUQPX_001034"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HHV9055808.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            42472..43245
                     /gene="zupT"
                     /locus_tag="ACUQPX_001035"
     CDS             42472..43245
                     /gene="zupT"
                     /locus_tag="ACUQPX_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HHV9055809.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(43303..43473)
                     /gene="yqiD"
                     /locus_tag="ACUQPX_001036"
     CDS             complement(43303..43473)
                     /gene="yqiD"
                     /locus_tag="ACUQPX_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HHV9055810.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(43734..44387)
                     /gene="ribB"
                     /locus_tag="ACUQPX_001037"
     CDS             complement(43734..44387)
                     /gene="ribB"
                     /locus_tag="ACUQPX_001037"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HHV9055811.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(44491..44639)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            44761..45051
                     /gene="ubiK"
                     /locus_tag="ACUQPX_001038"
                     /gene_synonym="yqiC"
     CDS             44761..45051
                     /gene="ubiK"
                     /locus_tag="ACUQPX_001038"
                     /gene_synonym="yqiC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HHV9055812.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11283 a        12069 c        11300 g        10620 t
ORIGIN      
        1 tggtgtcatc aaaagtgtct ttaagggtat agcagccgtt ttcgaccacc atctccagct
       61 cgcgcgtcca ctgttgccct tgcggcaacg gggaatgaat cacgttctgt tccgcaatac
      121 ggactttgtt gccatgcaat tgggtaatga tggcgacatg gccagtgtct ttaaattcac
      181 cgcctttatc ccagataaga agcgcacccg cgaccggcgc acgcggcgag ccgttaggaa
      241 atgcctgcaa tggcaggatg ttgtcattaa ccacttctcg caggaagcgc agcgagaaaa
      301 tctcccacgc catacccacg tcagtaaaga ccacaccgta attcagaaag agaaaacggc
      361 gagcaaattc aacgcattgc cacttgtggc ccatatattc gtcgtcgata tagctacgga
      421 atacggcgtc atcttcgtat tcctgcggat cgagagaact gtaatctgaa gagtagattg
      481 ctaccccacc tggggcgtag cccaataatg tcccgaacgg ggcatcctgg ctggtcgttc
      541 ctttgctcat cactttacct ttaacaatac aacctaagca gttggcgtaa ttgttgtgct
      601 ctgattacct gctcagcgac attaaccgga cagaggtcag actaatcata cacctcatcg
      661 cagcggctgg cgcaaagggt taaaaaattc acattctgta cagcaagtga cctgctacac
      721 tgcttcaaca ctaccactca gaaggcaact cactatgaca gacaatactt atcagcccgc
      781 gaaagtctgg acgtgggata aatccgctgg cggcgcgttc gccaatatca atcgcccgtt
      841 ttctggtccg acgcatgaaa aaacgctgcc cgttggcaaa catccattgc aactttattc
      901 gctgggaacg ccgaacggtc agaaagtaac gattatgctt gaggagctac tggcgctggg
      961 cgttactggt gcagagtacg acgcctggct gattcgtatt ggcgatggcg atcaattctc
     1021 cagcggcttt gtcgaagtga acccaaactc gaagatcccg gcgctgcgcg atcatacgca
     1081 taatccgccg atccgcgtgt ttgaatctgg ttcgatcctg ctttatctgg cggagaaatt
     1141 tggctacttc ctgccgcagg atctggcaaa acggactgaa acgttgaact ggctgttctg
     1201 gttacagggt gccgcgccgt tcctcggcgg tggttttggt cacttttacc attacgcgcc
     1261 ggtgaaaatt gagtacgcca tcaaccgctt taccatggaa gccaagcgcc tgctcgacgt
     1321 gctggataag caactggcgc agcataagtt tgttgcgggc gatgagtaca ccattgcgga
     1381 tatggcgatt tggccgtggt ttggcaacgt ggtgctcggc ggcgtgtatg atgccgcaga
     1441 gtttctggat gcgggaagtt ataaacatgt gcaacgctgg gcgaaagaag tgggcgaacg
     1501 tccggcagtg aaacgtgggc gtattgttaa ccgcaccaac gggccgctga acgagcagtt
     1561 gcatgagcgc catgacgcca gtgattttga gacaaataca gaagataagc gtcaggggta
     1621 agggttggtg ttcgtcgcag caagccatcc aggccggata aggcgttcac gccgcatccg
     1681 gcaatcgtgc acaatgcctg atgcgacgct gccgcgtctt atcaggccta caaaagctaa
     1741 tcatcacgcg ctggtaatgt cgtaatccat ttggcgcagt gcgtctaatg tggctttggc
     1801 ttcatcttcg tcgatgatgc tcatggcaaa tccgacgtgt accagcaccc actggcccag
     1861 cagatcggca gggttacctt cgcaaatcag ggcgatattc acgtcgcgct tgataccaca
     1921 tacttcaacc tgcgcaagct ggtgaatatc ttcaccgaca gccagcacct ggcctggaac
     1981 gccaatacac attgttaact ccggttattt cgattgccgg acaaacgcga aaaagaacct
     2041 ttagcgggtg ttccctctca ccctaaccct ctccccggag gggcgagggg accgtccgtg
     2101 cgctttttag gcgttgtcct cagtcttatt caacttcaat acttttgacg atcagcgaat
     2161 cgccggtatc gacacgcaac cgctcgccgt gacagagcgg acactgcgca tcgtgctgat
     2221 gaatctccac cacctggctg caatcccagc accaggcctg ggcgggttta tagacgatat
     2281 gtaaatcgca cccttgcgcc accgttccgc ggcagacaat ttcaaaacta aaacggacgg
     2341 cgctctcctc aacgcaggag agcgcgccaa tttccagcca cacggcggtg acgcgcttaa
     2401 catcgtgctg ctccgcctgc cgttggataa tttcaacggc gctctggcaa agcgacaact
     2461 catgcatttt cgcccctcct gcggccaaac agcagggcgc gacgccctgc gtgtggtaca
     2521 tccggattgg tgactggcaa cgaaaggatc attcgtgcgc agtcatccgt caggcgttgg
     2581 ccttcttcaa tcgacatgct gtgcgaaagc ggcgacatca gcgaacagga gaggtattgc
     2641 gaaacaccgt ccagttcgcc aacggtaaag gtcatggtgc catacggcag ttgcagacca
     2701 attttttcac tcactttgcg cagcggccag agttgatccg ggccggggaa gataactgca
     2761 ctcagcatcc acggggtgat cacacacccc gtccactgac cttcgaacag cgtaaaatca
     2821 gaaacataca ccggcattga aggatgcaga aaagagagat cgtacatcga acgccgggca
     2881 atttcttcaa acgctgcctg tacttgcgcc ttcggggagg tctggaaacc tgctatctct
     2941 tcagtcatga atcgcctccc gtgggatggc ttccacgcct gattcgcgca gcgcagccag
     3001 aacctgctca agcgcaggtt caatcattgc ttcaaccgtc ggcgttaagc cgatgtgtgg
     3061 ctccagcgat tccgggatca cgccgaccag ggtcagcttt ttcggaaact caccggtgaa
     3121 gcgcagggcc gacaatacgt cggccaggcc aagctgatgc ggagagattt tgttggtaaa
     3181 caacgccgga acttcttcat cccgcaggat catcatcgtt cccggcgtgt tctttttcga
     3241 cacaatggca tccgcaataa tcagatgatc gcgatttgcc atgtcgccaa gcagctccat
     3301 tcccgccgtg ccgccatcga ggatctcaac ataatccggc agaatgtatc gttgctctaa
     3361 cgcttcgaca atccgcacgc cgatggcttc atcggtcagc aaaatattgc cgacccctaa
     3421 gactaaaata cgcattacag aaccttcact gaaaccactt cgttgccgtc ggcatccact
     3481 acgtgtaccg cacaggccat gcacgggtcg aaggagtgaa tggtacgcac cacttccagc
     3541 ggtttattcg gatcggcaac cggtgtaccc accagcgact gctcgtaagg accgacgtca
     3601 tcattgaagt tacgcggacc agagttccaa gttgatggaa caaccgcctg gtagttgctg
     3661 atgataccgt ctttaataac catccagtga gagagcatac cgcgcggcgc ttcgaggaag
     3721 ccaacacctt tgaactcacc cgttgccgga atgttcggct tcacaaaggt ggtgtgatcg
     3781 cctttgccga tattggtgat cagtgcactg tattggtttt gcaggatatc ctgcaattca
     3841 cagcagtgaa cggtacgacc aataatacgg cccagcgtgg agtgcagctg tgccacttcc
     3901 agcgtgttgc cagtcagttt ctgataaatc gcaacgattt cattcagttt gttctgggta
     3961 gattcgcgac ctgccgccag tttcaccagc atattagcca gcggccccac ttctaccgtt
     4021 ttgccgtaga aagtcggtga tttcacccaa gaatatttcc cgtcgtcaga ccaaccatca
     4081 taagccggaa tggtggtacc ttcccacggc gcctgtggcg cttcgtcttt ataccaggag
     4141 tgcttcgcgc tttcctgaat gcctttgatc aggtattcat cggaatgaga agtgatcgga
     4201 cgatacgagg acagatccgc attctcaatg tagccgcccg ggaacaggaa gctgccgttt
     4261 ttgctgtcgg tcgggaattc cggcacgctc aggtagttca ccgcaccttt accgcgcgtc
     4321 agccattccg ggtagaacgc ggcgataacc gcggtatcaa ccttataaac ctgctcaaca
     4381 aagtcgctca gtttgtcgat gaaagacttg atgtacatca ggcgctcaag gttcaacacg
     4441 cccaacccgt cgaggttgat tgggttcgcg acaccaccca ctgccaggtt ctgaatgtgc
     4501 ggcgttttac cgcccagcag cgccacgacg cggttagcgt cacgctggca ctccagcgcc
     4561 tgcaggtagt gcgctaccgc aatcaggttc acttccggcg gcagtttcat cgccgggtga
     4621 ccccagtagc cattggcgaa aatacccaac tgaccgctgg caaccagatc tttgatcttg
     4681 ttctgaactt tagtgaactc ttccggactg ttcagatgcc aggtcgaaac gcctttcagc
     4741 atttcggagg ctttggttgg gtcagcttgc agtgcagaag tgatgtccac ccagtccagc
     4801 gccgaaagct gatagaaatg aacgatatgg tcatgcgtgg tgtgcgcagc cagaatgatg
     4861 ttacggatgt attgcgcgtt aaccggaacg tcgatattca gcgcactttc tgccgcacga
     4921 acggaagaca gcgcgtgagt ggtagtacat acgccacaga tacgttgcac aatcatccat
     4981 gcatcgcgcg gatcgcggtt tttcacgatc tcttccatgc cgcgccacat ggtaccggaa
     5041 gcccatgctt tcgaaacgac gccattttcg atttcgcaat cgatgcgtaa atgcccctca
     5101 atacgggtta ccggatcaat agtaattctc tggctcatgc tttgctcgcc tcatgacgat
     5161 tatgatcgtt ttgttttaaa ggaggaagta tcggcagtag acgaatgagt acgatgtaag
     5221 cgcaaatctc aatagccaca aaaccaatag aaatcaacag ttcttcccag gtcgggaagt
     5281 aggcgtaacc gccgcccggg ttgaatgcca ccaacgaata ggtcagacgc caggttgcac
     5341 aacctaacag tgcgctcagt gctgacagga acagcatgcg ggaatcatta cgcagcttcg
     5401 ccacacgcag aacgaccagc gggaagagca tcagcaggac ttcaatccag aacatcacgg
     5461 agtagaagtc accggcaaac gctaacgaca gcttgtcgcg atagataagc tcgccaaagc
     5521 gcagcccgat gaaaatcgcc agcaacacac tgatggtgtt ggtcagcttg acgaacaggc
     5581 tcttttcatc cggaccgttg ccacgcagac ccgcctgcac cagtgaacct tcaaagatga
     5641 caatcgagaa gcccatgatg aacgccgtca gcagcgagaa cagcggcaac atttcatagc
     5701 tctgccagag cggatgcacc ttgtagcccg ccgagatcat cagcgacccc attgaagact
     5761 ggtgcatggt cggcagcagc gcaccgagcg cgataatgaa gaacatcacc ttgttcagac
     5821 gttgtagcga caccttccag cccagacgtt caaacagtgc cggagcaaac tccagagcca
     5881 tcacgccgat gtagatggtc atacagaccg ccgtctcgaa cagtaccgag ttcacgttga
     5941 agtgacccgg aatgtagaag tacggcaggt tccagtagcg acccacgtcg atagtgatcg
     6001 acaagccacc cagtgagtaa ccaaacagac tcgccaacag cgccggacgc accagcggat
     6061 ggtattgccc acggttaaag acgtataccg cccacgccag cgcccagccg ccacaggcaa
     6121 agccggtgcc aatcagcagg tcaaacgcga tccacacgcc ccacgggaag ccgccgttca
     6181 ggtcagagac agagcccaga ccgaacacca gacgcttcac aatcaggagc atacagatga
     6241 cgattaacgg tccaaaaatc atgaccggtt tactgatgat tttgccgccc agcggttgtg
     6301 gatcatgact catgatcgtc tcctccgtcg tgatggtcgt ttttggtgtt gcgacgaacc
     6361 agcacggtta agcccgccag cacagccagt ggtagcatca tgcctttata cagggtgtgt
     6421 tgaatatttt cggaacgcgc accggtagaa agatcgtcca gtttcggcag gtcgagattt
     6481 tcataaggca cacccgtcag taccagaacc tgagtaccgc cgccctcttt ctcgccgtac
     6541 agatgcggat aatatttcgg caccgtatgc aggtaagtgt cgccagattt cagcgtctga
     6601 cgcggatagt ggtattcgct gccaggcttc agcgccagac gttttttcgc ctccgccatc
     6661 agctcttcac gcgtaccgaa aatcaccgcg cccgccgggc acacttctac gcagccaggc
     6721 agaccgcctt tatcgagacg ttccacacct ttctggttgc acagctcgca cttatgcagc
     6781 gcaccaaacg ggttgttgta gtcgtacttc ggcacgttgt acggacaggc gaccatgcag
     6841 taacggcaac cggtgcacac gtctttgtcg taatggacaa tgccggtttt cggatctttt
     6901 ttcagtgcag agaccgggca cacagagaca cagttcggat cgacgcagtg catacactgt
     6961 ttcttaatgt acgcgtagcc gttctcctcc tggtctttgt tgacccctgt gccgctggtc
     7021 cacacctgaa tgatgttatt ggtatacggc gacagtttgt cgttgttcaa ccaggtctgt
     7081 tccccttgcg ggttacgttc agggaagttg atatcctgac acttggtgac gcaagcctgg
     7141 cagcctacgc acaaggtcga gtcgtacaac atccccagcg atcccggaat tggcgggcgg
     7201 ttttcagcag ccgcatgact gacagacggc agcgcgcccg tcagcaatgc cccgcaggag
     7261 gctgctttaa taaaattacg tctgttcacg gttattctcc ccgtgagtca gcgttatctt
     7321 tcttttgctg acgacccagt tcacgcaccg ccatcacgct gacaccggca accagcccaa
     7381 ccacaccgcc gagcaaacca atagcgcctg cagagacgtt gccgccctct ttagcgttaa
     7441 catccggttt ctgtgaacgc ggagtctgat tttcgacgtt ggcaagctga tggatgcctt
     7501 tatggaagcc gatacctttt tcgttacagc catagcacgg gtgaccaatc gccaccggcc
     7561 acacaccgcc aacatcgcag aattgcagcg ttgagcagtt gccgtaagtt tctggccctt
     7621 tacagccgag gtggtacagg caccagcctt cgcggtggcc ttcatcaccg aactctttgg
     7681 caaaacgacc agcatcgaag tgcgggcgac gttcgcagtg ttcgtgaatc agacggccat
     7741 aggcgaaggt cggacggttt ttgtcatcca gtttcggcgg tttgccgtaa gtgatgatgt
     7801 gcgcaacggt cgcgaggaag ttgtgcgggt tcggcgggca gcccggaata ttgataacgg
     7861 ttttgcctgg cagaacttct tgcaagctga ctgcgccagt tgggttaact ccagctgcgg
     7921 caacaccgcc ccacgcggag caggaaccaa tagcgataat ggctgctgcg ccttccgccg
     7981 ctttgcggat gtgatccaca atcggctcac cggcaaccat gcaataaata ccgttatctt
     8041 ttaatgggat ggaaccatcc accactaaca catactgccc tttgtacttc tcgagagcgt
     8101 tatgtttgtt ctcttcgacc tgatgaccga aggcggcgga aagcacttcg tgatattcca
     8161 gagagatagt ctccagcacg aggttttcta ccgttggatg cgttgcacga agcagagatt
     8221 ccgtacaacc ggtgcactcc tgcgcgccaa tccagataac tggcggacgc tgcgggttag
     8281 taaccgattc ggccatctct gcagcggctt tgctacttaa ccccatggtg gcggctaatg
     8341 ctgcacaaag cttcatgaaa tcacgacggt taatgccgtg agaatggatg agggtgttat
     8401 ctccagtcat ttatagttat tccgttgcga agacctggca tatattttgc ctcaatcgca
     8461 aaatcaataa tgcgatcgat gcgccattta ccacacattt attatggtta tcgatatcat
     8521 gatactgcgg cggggcgcta aaacgaaggg aagaagcatt agtgtaatta attaaagcaa
     8581 gataatacgt atgtttgatc aattttcgag gcgataaccg gccataaaaa agcgcaacgt
     8641 agaacaggaa ttattctcac gaaagaaatc cattaccatc tctttatcca gtccatagcg
     8701 gcgcgtcaga atacccgctt cccaggcgct aagctagcga tctccggtct tttcgtacat
     8761 ccggtgtgag tagcctaata caaaaccgcg tttataatcg gcacagaatt ttgtcacatt
     8821 aaccgcactg tcggcatgcg ttgcttttaa tcccgccatt aagcctttac caaaatggtt
     8881 attcatcgtt cacctcattc gttatatatc gccatatata acgatatttt aggcaaagaa
     8941 cgacttttgc gcacgctatt ttttcatact tgtttaagac gtaatcccgt aagagataaa
     9001 cctatcagga gagatattta tcgaaccatt tcagtgtcct ttgccaggca agatcggcgg
     9061 cagatttgtc ataacggggc gtggaatcat tatggaatcc gtgattaacc cccggataga
     9121 tataagcctc ataaacctta ttattgtctt tcaacgccgc ctcgtaagga ggccagccct
     9181 cgttgattcg ggtgtccagt tccgcgaagt ggagtagtaa aggcgcttca atcttcgcca
     9241 catcggcagt gggtgcctga cgaccataaa acggcaccgc gcaggccagt tccggatacg
     9301 cgacagccgc cgcgttcgat acgccaccgc catagcaaaa tccggtaata cccactttac
     9361 ctgtcgcttg cggatagcgt tgcataaact caattgcggc aaagaaatca ttcatcagtt
     9421 tggttggatc aacctgctgt tgcagctcac gacctttatc atcatttccc ggataacctc
     9481 caacggaact taatccgtca ggtgccaggg cgatataccc cgccttcgcc actcgccgtg
     9541 ccacatcttc gatatacgga ttcagtccac gattctcatg caccaccacc acggctggcg
     9601 ttttgccgct catttttgcg ggtttcacca gataaccccg tacctcgccg tgaccatttg
     9661 gcgaaggata cgtgatgtac tcagcaacaa tctccgggtc ggtaaactct acctgagtcg
     9721 ccagcgcata atttggcttg agcaaatcaa acaacgctaa tgccatcatc ccgcccaccg
     9781 catacttcgc cgcaaggttg aggaactcac gtttcgaaat tttcccgtga gcgtaatagt
     9841 cgtagtaatc caacaactct tgtgggaaat ctttggcggt taaacgcggc atcgttacat
     9901 tcctcaactg gtgttttttt aagcaaagca taagcgcgtg tttttgccca gtttttcgtc
     9961 actctgtgag ccagactacg ggatacgcgc tggcgaatcg ctaaactaga aacattgttt
    10021 cgaaattgaa cggtggaaag gagaggtcat ggtctggtta gcgaatcccg aacgttacgg
    10081 gcagatgcag taccgctatt gcggaaaaag tggtttacgc ctgcccgcgt tatcgctcgg
    10141 tttatggcac aatttcggtc acgttaacgc gctggaatca cagcgtgcaa tcctgcgtaa
    10201 agcgtttgat ttaggcatta cgcactttga tttagccaac aattacgggc cgcctccagg
    10261 aagcgcagaa gagaactttg gtcgcctgct gcgcgaggat tttgccgctt atcgcgatga
    10321 actgattatc tctaccaagg ctggctacga tatgtggccc ggcccgtacg gctctggcgg
    10381 ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta
    10441 tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc
    10501 tgcgctggct catgcggtac aaagcggtaa ggcgctgtat gtcgggatct cctcttactc
    10561 gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag tggaaaattc cgcttttaat
    10621 tcatcaacct tcgtacaatt tactgaaccg ctgggtggat aaaagcggcc tgctggatac
    10681 cctgcaaaat aacggcgtgg gctgtattgc ctttactcct ctggctcagg gattgctgac
    10741 cggaaaatat ctcaacggca ttccgcaaga ttcacggatg catcgtgaag ggaataaagt
    10801 tcgtggtctg acgccgaaaa tgcttaccga agccaacctc aacagcctgc gcttattgaa
    10861 tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa
    10921 agatgatcgc gtgacgtcgg tattgattgg tgccagccgc gcggagcaac tagaggagaa
    10981 cgtgcaggcg ctgaataatc tgacatttag caccgaggag ctggcgcaga ttgatcagca
    11041 tatcgccgat ggcgagctga atctgtggca ggcgtcgtcc gataaatgac ctgttaataa
    11101 cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg acgagtcagt cggtcaagat
    11161 agcccatcac aaatgcagaa agcacaaacg tcagatggat aatgacgtac cacatcagtt
    11221 tgttatccgg gacatttttc gcatccataa agacgcgcag taagtgaatg gaagaaattg
    11281 ccacaatcga cgctgctact ttgtttttca gcgacgttgc gtccattttc cccagccagt
    11341 tcagcttctc tttgttctcg gagatatcca gctgtgagac gaaattctca taaccggaaa
    11401 acatcaccat caccagcaaa ccgccaacca gcgtcatatc caccagcgac agcaacacga
    11461 ggatcaaatc tgattccgcc atcgagaaga tattcggcag tacgtgaata atctcctgga
    11521 agaacttcag cgccagggca actaacgcca gcgaaaggcc aaagtacacg ggggcaagca
    11581 gccagcgaga agcatacatt gcattttcaa gaaaacgttc catacattcc tgtcgtgtag
    11641 ttaaaacggg caacagtata tcgcaattga gtaaacagca cgcaacagca aatcaacata
    11701 tacgttaaac ctttgtcgca taattctgag gtttagggat cttcctggtt cccttatcaa
    11761 cgcatcgaga gcgtctaccc ttaaaggacg ttgaacctga agggagaaaa acgatgtctc
    11821 atttaaaaga cccgaccacg cagtattaca ctggtgaata tcccaaacag aaacaaccga
    11881 cgccaggcat ccaggcgaag atgacaccga taccggattg cggcgagaaa acctatgttg
    11941 gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg gggcgattcc ggaataggtc
    12001 gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg
    12061 tggaagaaga agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg
    12121 ttctgctgcc aggcgattta agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc
    12181 acaaggcgtt aggcgggctg gatattatgg cgctggtcgc cgggaaacag gttgccattc
    12241 cggatattgc agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg
    12301 cgctgttctg gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtatcatca
    12361 ccacttcgtc aatccaggca taccagccaa gtccgcattt actggactat gcggctacga
    12421 aggcggcgat tctgaactac agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc
    12481 gggtgaatat tgtcgcgcca ggccctatct ggacggcact gcaaatttcc ggcggacaaa
    12541 cgcaggataa gatcccgcag tttggtcaga aaacgcctat gaagcgtgcg gggcaaccgg
    12601 cggaactggc ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag
    12661 aagtgcacgg cgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag
    12721 caaccgggca tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct
    12781 acaaacgcat gaagattcaa catgttgcaa tagattttgt aggcctgata agcgtagcgc
    12841 atcaggcatt gagcacctaa tgccggatgc atcgtgaacg ccttatccgg cctacaaacg
    12901 cacgaagatt caacatgttg caatagattt tgtaggcctg atgagcgtag cgcatcaggc
    12961 aattctactt tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc
    13021 cgcctgatgc agcgtatcca ttaccttcat caacgtctcg taatcgacgg ttttgtccgc
    13081 tcggaagaag atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat
    13141 tgtttcatcg gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag
    13201 ataaaccggt ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttcacctt
    13261 cacatctacc gtcgctaacg gtgccgccac cataaagata atcagcagaa ccaacatcac
    13321 gtcgataaac ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt
    13381 aagatgcatt gccatcggac attatcctgc gcgtaatttt tgtgcgacac gaaccggatg
    13441 cgcagcggcg ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc
    13501 aacatcaccc agcatcgctt taaagccgcc aatctggcgt gcaaatacgt tatagataac
    13561 gaccgcagga atcgctgcca cgaggccgat tgccgttgct aacagagctt ctgcgatacc
    13621 cggcgcaacg actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat
    13681 gatgccccag accgtaccaa acagaccaac gaacggagaa atcgcgccga tggttgccag
    13741 atagccgtta ccgcggccca tttgacgacc cactgcggcg acccgacgct ccagacggaa
    13801 gctggtacgt tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt
    13861 ctgcgcttca ttgagcaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc
    13921 gttggcctgg tttaaggaac gtgcttcagc cagcagttgc tgctcgcgct taaggcgacg
    13981 cttctgattg aagaactcta cgctcttact gaagaagatt gcccaagtga ctacggaggc
    14041 caaaataagc ccaatcatca cgcacttaac gacaatatcg gcgtgctgat acatacccca
    14101 gacggaaagg tccgtctgca ttaaattatt acccactgtg tatctccagg acgcaagtca
    14161 caaaatctgc gcataataat atcaaaacga cgtcgaattg atagtcgttc tcattactat
    14221 ttgcatactg ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca
    14281 gcacgccatt tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata
    14341 ttcatgctag tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa
    14401 agcttgatac tcaactggtg aatgcaggac gcagcaaaaa atacactctt ggcgcggtaa
    14461 atagcgtgat tcagcgcgct tcttcgctgg tatttgagag tatggaagcc aaaaagcacg
    14521 cgacgcgcaa tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt
    14581 tttccttaca acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct
    14641 gcggggcggc agcggttgct aattcgattc ttgcttttgt cgaacagggc gatcatgtgt
    14701 tgatgaccaa caccgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac
    14761 tgggcgtaac gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc
    14821 agccaaacac taaaatcgtg tttctggaat cgccgggttc catcactatg gaagtccacg
    14881 acgttccggc gattgttgcc gccgtgcgca gtgtggtgcc ggatgccatc attatgatcg
    14941 acaacacctg ggcagccggt gtgctgttta aggcgctgga ttttggcatc gatgtttcta
    15001 ttcaggccgc caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtgt
    15061 gcaatgcccg ttgctgggag cagctacggg aaaatgccta tctgatgggc cagatggtcg
    15121 atgccgatac cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc
    15181 aacatcatga aagcagtctg aaagtggccg aatggctggc agaacatccg caagttgcgc
    15241 gagttaacca ccctgccctg cctggcagta aaggacacga attctggaaa cgagacttta
    15301 caggcagcag cgggctattt tcctttgtgc ttaagaaaaa actcaatgat gaagagctgg
    15361 cgaactatct ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat
    15421 cgttgatcct ggcaaatcaa ccagaacata tcgccgccat tcgcccacaa ggcgagatcg
    15481 attttagcgg gaccttgatt cgcctgtata ttggtctgga agatgtcgac gatctgattg
    15541 ccgatctgga cgccggtttt gcacggattg tataacattg ccacttttgg acaattttgc
    15601 agacatttta ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga
    15661 ttcaggagta caataggcag ataaaggctt aaacgctgtt ccacaggaaa gtccatggct
    15721 gttattcaag atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct
    15781 catattgtta gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg
    15841 ctgcccgcct catttttgcc aggcgacagc ttgttgatac tggcaggcgc attgattgcc
    15901 caaggggtta tggattttct gcctacgatt gcgattctga ccgccgcagc aagtctgggc
    15961 tgctggctaa gttatattca ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg
    16021 ctggcacagc ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt
    16081 ctgctggcgc tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg
    16141 gcgggaattt ccggtctgcc aaaccgccgc ttccagtttt tcaactggtt aagtggattg
    16201 ctgtgggtca gcgtggtaac cagttttggc tatgccttaa gtatgattcc gttcgttaaa
    16261 cgccatgaag atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct
    16321 ggcttgttag gcacgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt
    16381 tccccgttcc cggctgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct
    16441 tgccgcatct tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga
    16501 tgcgctttca tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat
    16561 catcatccgc gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt
    16621 gacagactta aaattatggt ggaacgccga tacgctcatg ttggcttctg ccgccagttg
    16681 ctcgacgctc aggttttcgg tgtatttatt ctcaattcgt ttcagcacgc ggctaatcag
    16741 actgaagtga gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag
    16801 cacgtagtac agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg
    16861 ctccatcacg tcgagtaacc gctccgccgc gcataaaatc tcttctgata acgtggcgga
    16921 gttaatcccg ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa
    16981 cagttcctgt aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga
    17041 cgtggcatag gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc
    17101 atcataacga aacacgcgtt cattgatata accgatttta tgcccggaaa agagaattat
    17161 gatgccaggc tcgtacatca ccggtgtacg tgcgaaaggc gtctcgccat acaacaaacg
    17221 cacatcgggc aacagttctg acaaactatt ttctttattt ttcagtttat taactttatc
    17281 cgccagcaag cgacaaatct cttcacgttt catatcgcgt aatttcttag gaataatgcg
    17341 gcaatttgat tgtgcgcaat tttgtagcat ttctccagca ctctggagaa ataggcaaga
    17401 cattggcaga aatgagcatt gagagccagg gcgctggcga tcacaatgaa aaacatcagg
    17461 cagatcgttc tctgccctca tattggccca gcaaagggag caagtaatga acaactttaa
    17521 tctgcacacc ccaacccgca ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca
    17581 aattcctcac gatgctcgcg tattgattac ctacggtggc ggcagcgtga aaaaaaccgg
    17641 cgttctcgat caagttctgg atgccctgaa aggcatggac gtgctggaat ttggcggtat
    17701 tgagccaaac ccggcttatg aaacgctgat gaacgccgtg aaactggttc gcgaacagaa
    17761 agtgactttc ctgctggcgg ttggcggcgg ttctgtactg gacggcacca aatttatcgc
    17821 cgcagcggct aactatccgg aaaatatcga tccgtggcac attctgcaaa cgggcggtaa
    17881 agagattaaa agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga
    17941 atccaacgca ggcgcggtga tctcccgtaa aaccacaggc gacaagcagg cgttccattc
    18001 tgcacatgtt cagccggtat ttgccgtgct cgatccggtt tatacctaca ccctgccgcc
    18061 gcgtcaggtg gctaacggcg tagtggacgc ctttgtacac accgtggaac agtatgttac
    18121 caaaccggtt gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc tgacgctaat
    18181 cgaagatggt ccgaaagccc tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat
    18241 gtgggcggcg actcaggcgc tgaacggttt gattggcgct ggcgtaccgc aggactgggc
    18301 aacgcatatg ctgggccacg aactgactgc gatgcacggt ctggatcacg cgcaaacact
    18361 ggctatcgtc ctgcctgcac tgtggaatga aaaacgcgag accaagcgcg ctaagctgct
    18421 gcaatatgct gaacgcgtct ggaacatcac tgaaggttcc gacgatgagc gtattgacgc
    18481 cgcgattgcc gcaacccgca atttctttga gcaattaggc gtgccgaccc acttctccga
    18541 ctacggtctg gacggcagct ccatcccggc tttgctgaaa aaactggaag agcacggcat
    18601 gacccaactg ggcgaaaatc atgacattac gttggatgtc agccgccgta tatacgaagc
    18661 cgcccgctaa gctttttacg cctcaaactt tcgttttcgg gcatttcgtc cagacttaag
    18721 ttcacaacac ctcaccggag cctgctccgg tgagttcata taaaggagga acgtatggct
    18781 aatccaaccg ttattaagct acaggatggc aatgtcatgc cccagctggg actgggcgtc
    18841 tggcaagcaa gtaatgagga agtaatcacc gccattcaaa aagcgttaga agtgggttat
    18901 cgctcgattg ataccgccgc ggcctacaag aacgaagaag gtgtcggcaa agccctgaaa
    18961 aatgcctcag tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac
    19021 aagcgccccc gcgaagccct gctcgacagc ctgaaaaaac tccagcttga ttatatcgac
    19081 ctctacttaa tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc
    19141 atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc
    19201 catcacctgc aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa
    19261 cttcatccgc tgatgcaaca acgccagcta cacgcctgga acgcgacaca caaaatccag
    19321 accgaatcct ggagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt
    19381 cgcgatctgg cagataaata cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg
    19441 gatagcggcc tggtggtgat cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt
    19501 gatgtctggg atttccgtct cgacaaagac gaactcggca aaattgcaaa actcgatcag
    19561 ggcaagcgtc tcggtcccga gactgaccag ttcggcggct aacgtgcaaa ttctcccggt
    19621 ggcggtaatg ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc
    19681 gttgttactc ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga
    19741 agctcttaaa agaacaactc atctatatcc gggataaacg caacggagag gtgaaaaaca
    19801 gatgaaaata atacttctgt ttttagcagc cctggcaagt tttaccgtac acgcgcagcc
    19861 tccttcacag accgtagaac aaacagtccg gcagatttat cagaactata aatcagatgc
    19921 cagtacgcct tatttcggtg aaaccggaga gcgggcgata acttcagcgc gtattcaaca
    19981 ggcgcttacc ctgaacgaca atcttacact accgggcaat attggctggc tggattatga
    20041 tccggtttgt gattgtcagg attttggcga tctggtgtta gaaagcgtag cgataaccca
    20101 aactgacgtc gatcatgccg atgccgttgt gcgctttcgt atctttaaag atgataaaga
    20161 aaagaccaca cagacactga aaatggtggc ggaaaatggt cgttgggtca ttgatgatat
    20221 tgtcagcaat catggcagcg tcttacaagc agttaatagc gagaatgaaa aaacgctggc
    20281 cgctttagct tcgttgcaga aagaacagcc agaatccttt gttgctgaac tctttgaaca
    20341 tattgctgat tatagctggc cgtggacgtg ggtggtttcc gactcttacc gccaggcggt
    20401 taatgccttc tataaaacca ccttcaagac ggccaataat cccgatgaag atatgcaaat
    20461 agaacggcaa tttatttacg acaatccgat ctgttttggc gaagagtcgc tattttcacg
    20521 cgttgatgaa attcgagtcc tggagaaaac cgccgattcc gcccgcattc atgttcgttt
    20581 tacgctgacc aatggcaaca atgaagagca agagctggtt ttacagcggc gcgaaggtaa
    20641 gtgggaaatc gctgatttta tccgcccgaa cagcggcagc ctacttaagc agattgaggc
    20701 aaaaaccgcc gccagattaa agcaatgagc tgaattaaat aacaattagc cggaacaata
    20761 aataaaaggg aacactatat gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc
    20821 tgcttgctca gtggctgcgt caatgagcaa aaggtcaatc aactggcgag caatgtacaa
    20881 acattaaatg ccaaaatcgc ccggcttgag caggatatga aagcactacg cccacaaatc
    20941 tatgttgcca aatccgaagc taacagagcc aatacgcgtc ttgatgcaca ggactatttt
    21001 gattgcctgc gctgcttgcg tatgtacgca gaatgataaa aaaatccccg gcagcatgtc
    21061 agttgccggg gatttttttt aacgtccaac cgccgcttta gggcgtttct tcgcaccagc
    21121 attcaccgga cgagattgcg tagacgacgc tttttttgcc gtagcaggcg tctgacgctg
    21181 agtcgccatc ggcgtatgtt tagtcaacgc cggacgggta ttgcggttct ggcgacgagc
    21241 ttcacgcatc tcttcaatgg ttggcgcagg cactaagcaa tcgcgacggc tgccaatcag
    21301 atgctttttg cccatcgctt ccagcgcctg gcggattaac ggccagtttg ccggatcgtg
    21361 gtaacgcaac aacgctttat gcaaacgacg ctgtttgtcg cccttcggta cgaagacgtc
    21421 ttcgctctta taaccaatct tcgccagcgg gtttttcccg gtgtaataca tggtggttga
    21481 gttcgccagc ggcgatgggt agaagttctg tacctggtcg agacggaaac gatgcttttt
    21541 cagccacagc gccaaattca ccatatcttc atcacgcgta ccggggtgcg cagagatgaa
    21601 atacgggatc agatactgct ctttacctgc ctgtttcgag taggtatcga acagctcttt
    21661 aaagcggtca tagctgccca tgcccggctt catcatcttc gacaacggcc cttcttcggt
    21721 atgttccgga gcaatcttca gataaccgcc gacgtgatgg gtcgccaact ctttgatata
    21781 gcgcggatct tccacggcga tgtcataacg tacgcctgag gcgatgagga tttttttaat
    21841 gcctttcaga tcacgcgcac ggcgatagag gttgatcgtc ggttcgtggt tagtgtccat
    21901 gtgcggacaa atatccggat aaacgcacga caaacggcga caagtttgtt cagcgcgtgg
    21961 cgatttgcag cgcaacatat acatgttggc agttggccca ccgagatcgg aaatcacgcc
    22021 cgtaaaacct ggaacggtgt cgcggatcgc ttcgatctca ttaatgatcg aatcttccga
    22081 acggctctga ataatgcgcc cttcgtgctc ggtaatagaa cagaaagaac agccgccaaa
    22141 gcagccgcgc ataatgttga ccgaaaaacg gatcatttcg taagccggaa tacgggcatt
    22201 gccataggcc ggatgtggca cgcgcttgta cggcagcgca aaaacgctgt ccatctcttc
    22261 ggtagaaagc gggatagcag gcgggttgat ccacacatag cggtcaccgt gtttttgcat
    22321 caatgcgcgg gcgcagcctg ggttggtttc gtggtgcaga atgcgcgaag catgggcgta
    22381 cagcacttta tcgcccttca ctttctcgaa agaaggcagc aatacgtagg ttttttccca
    22441 cggtttctgg cgcggtggct gcacgattac agctttcgct tcctgctttt tcggcgcaac
    22501 aggtttgtta tccgcacacg gcaaatcttc gccatacgga tgcgggattg ggtcgatttt
    22561 ccctggggta tcaagacggg tggaatccac accgctccag ccaggcagcg cctcttttac
    22621 gataattgcg gtattacgca catcgcggat ttcactaatc ggctcgccca tcgccagacg
    22681 atgcgctact tccaccagcg gacgttcacc gttaccaaac atcagcatgt cggctttcga
    22741 atctaccaga acggaacggc gcacggtatc ggaccagtaa tcataatgcg cggtacggcg
    22801 caggctagcc tcaataccgc cgaggatcac cggcacatct ttccacgcct ctttacaacg
    22861 ctgggtataa accagcgtgg cgcgatccgg tcgcttaccc gcgacattat ctggcgtgta
    22921 ggcatcatca tgacgtaaac ggcgatcggc ggtatagcgg ttgatcatcg aatccatgtt
    22981 gccagcagta acaccaaaaa acagattcgg tttacccaga cgcataaagt cgtctttgct
    23041 gctccagtcc ggctgggcga tgatcccgac gcgaaagccc tgcgcttcca gcatacgacc
    23101 gcaaatcgcc atcccgaagc ttgggtgatc gacatacgcg tcgccagtaa ccaaaatgat
    23161 gtcgcagcta tcccagccaa gttgatccat ctcttcacga gacatcggca aaaacggtgc
    23221 cggtccaaaa caggcggccc agtactgcgg ccaggagaac aggtcgcgat ccggttggat
    23281 cagggagata gagctcattt tgcttccaga aatgataaaa aaataatcaa aggccgggga
    23341 ttataagccg gaacgaaaga gaaatcgaaa ggtattccat actcgccctc ctcgggcgag
    23401 tatgaagatt acggtaccgg attgaccaac agttgcccaa tcgagccacg gtccgccatt
    23461 tccagcgtct gactgttgaa gtagaacggg aagtgcgccc aggaaggctg accgaaataa
    23521 acaagcagct ccacttgtcc gtcaacccaa acggtatctt tccagcctct gtcttccggg
    23581 aacggcatcg cgccattcac gttacggatc tggaacatta cgccttcaat atggaacgct
    23641 tgcggctcgt ccgcgcgtac cgtccagcgt tcccacgttc cttgctgcgc ggtgacatca
    23701 atacggttga cgtcccacag ctgtccatta atacccgggt catcacccag actgatatcg
    23761 cgactgcgaa ttggcgaacc agccatgatt tcagttggca gcaagcgcat cggaagactg
    23821 tctgtgacca gcggcagaag gccggttggg cgcagcgtta gcaccaaggt agaaaccaga
    23881 atactggatg gctcaaagaa gccgcgaata cgatcaacaa tgctcgccgc ttcgccacag
    23941 gtgatcgaca cttcatcgcc gttgctcata tccaccagaa tctcgcggcg ctcgcccggt
    24001 gccagcgaaa gttgcttcac cgatacagga gcaggcagga atccctgatc gccagaaatc
    24061 acatgtaacg ggcaaccatc gctcatctgt agttgatagc gacgagagtt cgacgcgttc
    24121 agcagtcgca agcgcaccca gccacgcgag acttcaacgt acgggctttg tacaccgtta
    24181 accagcagcg tatcaccaac aaagccgccg cttcccggtt cgttgtattc tggcgtacca
    24241 aagttatcca gccgtttatc ctggataatg accggaaaat catccacacc ataatggttg
    24301 gggataggca gcgacttgct gacttcatct tccaccagcc acattccggc aaggccgtta
    24361 tagacctgct gagccgtgcg gttgggagta ttggcgtgat accacagagt agctgcgttc
    24421 tggcgaatag gcagtacggg tgcccagtca gcgtttggcg acatcatccg tgccggaccg
    24481 cccatcagcg ggcctggtac ctgtagcccg gccaccgtca ttgagacatt ttctgtcagg
    24541 cggttgctgt aaataagctt aacatcgtcg cccttccaga cgcggatagt cggccccagg
    24601 taacgaccat tgattcccca gaccgacgcg cgtgtccctg gcgtaaatga ccagtgcgca
    24661 cgttgtacag tcataaacag cggttgccca cggcgagatt caagtagcgg cggaacgggt
    24721 agcggttgct gttgcccggc tgcgctggcc ttcaggggaa cagcgcctgc acaaagtgca
    24781 atccccgatg cctgaatgaa ctgacgccga ctgagtgaca tatttgctcc atgtaaaact
    24841 ggctaaagta ataccgtgca ggaaagtgtt ccccgcaaaa atacagattg ccgacttaaa
    24901 cttttccggc ggcttcgcgt tctgcgactt ctttatcgag ctcggcgatt ttttgttcca
    24961 ttatcgaacg acaatgggca gccagctcac gaacctgatc tttgccatac tgactgacgt
    25021 caattggcgg cagcatttcg acaatcacca gaccgttgtg cagtcgatta agattaatct
    25081 tattcgaagt tgtagagacg cacacgggaa taatcgggac gcccgccgca attgccgcgt
    25141 gaaatgctcc agtcttgaac ggtagcaggc cgcgaccacg gctgcgggtt ccttccggga
    25201 acatccagat ggaaatacgg cgttttttga aatgattcac gacttccgca atggtgccgt
    25261 gagctttagt gcgattgttt ctgtcgatca ataagttgcc ggttaaccag tacaactgcc
    25321 cgaagaaggg gatccacagc aagctctttt tacctaccgt caccgtcggc ggttgcacaa
    25381 tgttcgatgc tgtcaccatg tcatagttgt tctggtggtt agcgatatag atagcattgc
    25441 cgtagctttc agcatctgca ggtttgcggc actcaacttt caggccgaac agtggcgcaa
    25501 gacggccaaa catatgccca aaggtggcca catgtttcgg gttacgcggg ctgaaaaggc
    25561 agtaaatgga gccgaataca cagactaaga tgctgtaaat cacggtaata ataagacgaa
    25621 agatatatag cataacaccc tctgaaggtc tgagagactg gcattgtacg tcacctgaaa
    25681 tcaggttagg gcattgttaa aagccgctct ctggaaaagc gtattgttaa tcgcaacgca
    25741 tgaataaaca acggtttcaa gggaattttt atcgtttccg ggggttgaat acgctgccgg
    25801 atgacgactt aacgtttcat ccggcgttcc ttgctagcgg gaggaaacag cgccctcccc
    25861 ggcatattac tcttcgctat caccgctgct ggcacggcga ggagagtcga tctcaacacg
    25921 atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcgc cagtgacttt
    25981 ctgtagctct tccggacgca gtttaatttt gcgtttccca acatgaatgg tcagcgtgct
    26041 ttgcggcggc agaacgtaca gttgcgccag accatcctcg ccacgcgcgg cttctgccga
    26101 tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat cgcttaccgg
    26161 gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag catcttcaat
    26221 caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct tacccgcacg
    26281 gttacgcgcc accagatcgt taaaggtgca gacgaaaccg taacccgcat cggaagccat
    26341 cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc caggcggcaa
    26401 cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg taatcgggtc
    26461 gatggcatag ctacgaccgg tggaatcaac aaacactacc ggttggttac tcttaccttt
    26521 caccgccgct ttgaagctat cacccgcttt ataattcagg cccggcgcgt cgatatcatg
    26581 gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag gttcagacgg
    26641 cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg gcgaacgacg
    26701 atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt tattcatttt
    26761 acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct cactctgctc
    26821 accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt cgaggatcgc
    26881 ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg gttcatcttc
    26941 gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca aaccttccag
    27001 gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc gcacggtatc
    27061 gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg ccggacgacc
    27121 atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg tggtagcgaa
    27181 gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa tcaccagacg
    27241 ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca gctttttgtt
    27301 gcgcatttgc gcagcaattt gctccagtac gcgcgcacct gaaacctgat gcggtaatgc
    27361 gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgca ctgaaccacg
    27421 tccgttctcg tagattttac gaatctcggc gcgcgaagtg ataatttccg cttcagtcgg
    27481 ataatccggc ccctgcacga tatccagcag ctgatcgagc gtggttttcg gctggtcgat
    27541 taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat cggtcgccat
    27601 gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacggg caggtagcat
    27661 tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc cctgccccag
    27721 ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac gcattgccgc
    27781 gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca gcggataacg
    27841 gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgctat cgccgtgcgg
    27901 atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt tggcgctggc
    27961 attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt tcagaccatc
    28021 accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta agtaggcgtt
    28081 ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt ctgattcctc
    28141 aacttattcg ccagccttca aaggggcaat attgccggag atactacatt atctgccgcg
    28201 tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg gcgataaagg
    28261 catcaatgtg ccttgatata caactctttg ctgtagactt gatcggcata gagggcagca
    28321 caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg attcgagaag
    28381 ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgactg taagtatacg
    28441 gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca taagtgcggc
    28501 ttgctcatgg atacccccga gacggcttac ggcgcaccgg ataataaatt tcgcgtatac
    28561 atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg acattgttcg
    28621 ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca tcacgccaac
    28681 ggcacataaa catccgtttc cagtcgctgc tctgtcaccc cttcggcaag attggtgtag
    28741 tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca gtggcgaata
    28801 atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc cacggcataa
    28861 cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat cggttcgcta
    28921 acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg gtcgtcccag
    28981 gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt acgccacatg
    29041 ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg caacatcgcc
    29101 acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt cattttgcct
    29161 ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg gatttacttt
    29221 gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt caccctgaat
    29281 ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtgat cgatatcaac
    29341 attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc gttcaacgat
    29401 attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgtttt ctacagtcgt
    29461 tacgctgccg ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg atgggccaga
    29521 aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta ctgcgattac
    29581 tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag catttaacaa
    29641 gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta cagagcgcgt
    29701 tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa tgcgaatttt
    29761 actgatagaa gatgacatgc tgattggcga cggcatcaaa actggcctta gtaaaatggg
    29821 ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt atagcgcacc
    29881 ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg atattttgcg
    29941 cgaatggcga gagaaaggtc agcgtgagcc ggtactgaac ctgaccgcgc gtgatgcgtt
    30001 agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta aaccttttgc
    30061 gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg gccaggccag
    30121 caacgagctg cgccacggta acgtcatgct cgaccccggc aaacgtatcg ccacgctggc
    30181 tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac tgatgcgtaa
    30241 cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct gggacgaaga
    30301 ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac tcggtagtga
    30361 ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa atttacccaa
    30421 cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc ggtgacctgg
    30481 ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga attgttcgac
    30541 acccaactga tgctgtttgc caaacggtta agtacgctcg atctcaacga aatcaacgcg
    30601 gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga tgacgatgcg
    30661 ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg cgataacgga
    30721 gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact ggtcggtgaa
    30781 gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg catcgttgtt
    30841 ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg gcaattgatc
    30901 ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact gggtcgtgaa
    30961 ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga ctcggaaaaa
    31021 ccactaaacg cgactggcgt acccagcgaa gtgcggccac tggttgagtc gctaaatcaa
    31081 ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc cgatgcagct
    31141 cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc gcagctctct
    31201 gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg gatcgatcgc
    31261 gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact ggataacctt
    31321 caggacgtcg cggagatccc gcttgaagat ctcctacaat cgtcggtgat ggatatttac
    31381 cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca cggcatcaaa
    31441 cgcaccggac aaccgctatt gctaagtttg ttggtgcgaa atttgctgga taacgccgtg
    31501 cgctacagtc cacagggcag cgtgatagac gtcacgctga atgctgataa tttcatcgtg
    31561 agggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga acgcttctat
    31621 cgcccacccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt ccagcgaatc
    31681 gccaagctgc atgacatgaa tgttgagttt gggaatgcgg aacaaggtgg atttgaggcg
    31741 aaggtaagct ggtaacttct ctgtctatga gagccgttaa aacgactctc atagatttta
    31801 ctaatagcaa aatataaacc gtccccaaaa aagccaccaa ccacaaacca gacaaataac
    31861 gagcgagaat acatatagcc aaaactcgaa catttcccgc ataaagagtt ttcttaagat
    31921 aagaataata agtggcgtaa gaagaaaaaa tgctgcaatc attgcttgca atatacgatc
    31981 ttcgcctgaa acggaaggta tactgcaaat attgcctccc gactcaagca tccagtcata
    32041 cttattgaaa gcaaaaaaag aatctattaa ccaataagta agcattatat atagcaatgc
    32101 ttcaaatatt gttttaattt tctgctttaa catatatcag tacgctcata aaaatccatt
    32161 tataattatc aacatgacac gttgatattt tcatctttta cttattcaaa gccatatttt
    32221 caatccccaa aaaaaccgaa ccaaacgtgt aaattattcc ggtgcgagta agaattatga
    32281 ggaatggcta tcagtattgt cattttcaga aaatatttat cctgcatcgg tgagtcagag
    32341 ctagatcaga cttttgctaa attcgcaaaa gactttgcac attttgctaa tttcaccgta
    32401 ccgctctgtg acgtactata gtcgacaaac gtctcacctt gaggttaaaa aatgagcaac
    32461 atcctgatta tcaacggcgc gaaaaaattc gcccactcca atggtcaact gaacgacacc
    32521 ctgaccgaag tcgcggatgg cacactgcgc gacctcgggc atgatgtccg catcgttcgc
    32581 gccgacagcg actacaatgt caaagcggaa gtacaaaact ttctctgggc tgatgtggtg
    32641 atctggcaga tgccaggctg gtggatgggc gcgccgtgga cagtgaaaaa atatattgat
    32701 gatgtattca ccgaaggtca cgggactctg tatgccagcg atggtcgtac ccgcaaagat
    32761 ccgtcgaaaa aatacggttc cggcggcctg gtacagggca aaaaatatat gctttctcta
    32821 acctggaacg caccaatgga agccttcacc gaaaaagatc agttcttcca cggcgttggc
    32881 gttgacggtg tgtatctgcc gttccataaa gcaaaccaat tcctcggtat ggaaccgctg
    32941 ccgacattta tcgctaatga cgtgataaaa atgcctgatg ttccccgcta tactgaagaa
    33001 tatcgcaagc atcttgtgga aatttttggt taactagagc tcaggcttta gaaggagtta
    33061 accatgctta ccgtaatcgc agaaatccgt actcgtcctg gtcaacatca ccgtcaggcg
    33121 gtattggatc agtttgctaa aatcgttcca accgtactga aagaagaagg ttgccacagc
    33181 tatgcgccaa tggtggattg cgctgctggc gtgagtttcc agtctatggc accggattct
    33241 atcgtgatga ttgagcagtg ggaaagcatc gcgcaccttg aagcgcatct gcaaaccccg
    33301 cacatgaagg cgtacagcga agccgtaaaa ggcgacgtgc tggagatgaa tatccgtatt
    33361 ctgcagccag ggatttaatc ctgccttgtt tgcccggcca tcctgaccgg gcaatgttct
    33421 ttcctttaaa cctcaatctc cgccatgtcg cctttctctt gcaaccagtt gcggcgatct
    33481 tccgagcgtt tcttcgccag cagcatatcc atcatcgcgt cagtacgctg atcgtcttca
    33541 tcatcgatag tcaactgcac cagacggcga gtgttcggat caagcgtggt ttcacgcaat
    33601 tgcatcgggt tcatttcccc cagaccttta aaacgctgga cgttcggctt gcctttcttg
    33661 cgttttaatt gctcaagtac gccctctttc tcttcttccg tcagcgcgta ataaacctct
    33721 ttcccgagat caatacggta gagcggtggc agtgcgacgt aaacgtgacc gtgtttcacc
    33781 aacgcgcgga agtgttttac gaacaaagcg cagagcagcg tggcaatgtg cagaccatca
    33841 gagtccgcat ccgcgaggat acagattttg ccataacgaa gctggctcag atcgtcgctg
    33901 tcaggatcga taccgatcgc taccgaaata tcatgcactt cctgcgaggc cagcacttcg
    33961 tcggaagaaa cttcccaggt gttaaggatc ttacctttca gcggcatgat cgcctgatat
    34021 tcacgatcgc gcgcctgctt ggcagatccg cctgcggagt caccttccac gaggaacagt
    34081 tcggtacggt taaggtcctg cgcggtacaa tcagccagtt tgccaggcag cgccgggccg
    34141 ctggtcagct ttttacgcac cactttttta gccgcacgca tacgacgctg ggcgctggaa
    34201 atcgccatct ctgccagcaa ctccgccgcc tgaacgttct ggttcagcca caggatgaag
    34261 gcatctttca ccacgcccga aacgaatgcc gcacactgac gcgaagagag acgctctttg
    34321 gtctgcccgg caaactgcgg atcctgcatt ttaaccgaca gaacataggc gcagcgatcc
    34381 cagatatctt ccgccgacag cttcacgccg cgcggcagaa tgttgcggta ttcacagaac
    34441 tcacgcatcg cgtccaacag gccctgacgc agaccattaa catgggtacc gccctgcatc
    34501 gttgggataa ggttgacgta gctttcggtc agcagttcac cgccttccgg cagccacagt
    34561 agcgcccagt ccacagcttc agtatcgcca gcgaaattac cgataaacgg tttttctggc
    34621 agcgtcggta aaccgtttac cgcttccgcc aggtaatcat tcagaccgtc ctgatagcac
    34681 cagcgctgtt cggtgttgtt gatctcatct ttaaaggtaa tctcaacgcc ggggcacagt
    34741 actgctttgg ccttcagcac atgcgtcagg cgtgaaacag aaaaacgcgg gctgtcaaag
    34801 aaggtttcat ccggccagaa gtgcacgctg gtaccggtat tgcgtttacc gcaagtgccg
    34861 acaacctgta aatcctgcac cttttcgcca ttttcaaagg cgatgttata aacctgacca
    34921 tcgcggcgca cgttaacttc tacgcgcttc gacaaggcgt taactaccga aatccccacg
    34981 ccatgcaggc cgccagagaa ctggtaattt ttgttagaga atttaccgcc tgcatgcaga
    35041 cggcaaagaa tcagttcaac cgccggtaca ccttcttccg ggtgaatatc caccggcatc
    35101 ccgcgcccat cgtcaatcac ttctaacgac tggtcagcat gtaagattac atccacgcgt
    35161 tttgcgtgac ccgccagtgc ttcatcgaca ctgttatcaa taacttcttg ccccaaatgg
    35221 ttagggcgag tggtatcggt atacatcccc ggacggcggc gaaccggctc aagcccggtg
    35281 agtacctcaa tggcatcagc gttataagtt tgcgtcatgg tttaagttag caattcgagt
    35341 tgatcgtcag agatggtgca gaccaagaaa atcgacgatc gggttgaaat aatcttcgaa
    35401 gcccgtgaat gcgtggttgc cgccttctat gacagtctgg cggcaggaag cgtagtacgc
    35461 caccgcctgg cggtaatcca gcacttcatc tcccgtctgt tgcagcagcc agatcaaatc
    35521 cggcgcttcc agcgggtcaa tctgcatgac tttgagatcg taaatatggc gtgactctag
    35581 cacatattgc tgcccggtgt aggggttctc gttctgaccg agatagtccg tcagcaattc
    35641 aaacgggcgt accgccgggt ttaccaccac tgcgggcagc ataaaacatt gcgacaacca
    35701 ggtggcgtaa tatcccccca gtgacgaacc gacaataccc agcgaatcgc cgccatgttc
    35761 caggacaatg gattccagca gttctgccgc gtcggaagga tacggcggca actgcggaat
    35821 gatcatctca acgtcagggt gatgttccgc cagccagttt tttaacaagc tcgcttttgc
    35881 agagcgcggc gagctgttga aaccgtgtaa ataaagaagc gtagacatca gtagccttct
    35941 gaagcggtat caggttggaa acgtgtgtcc gccaggcgat gcacctcggt ggtcagcgtg
    36001 ccatcagcat gtaactcgag agtacgccag ccgggcgcga tagtatccag cgtaaagttg
    36061 gaacagtgcg gcttaaactg cacacaggtc gacggcgttg ccagcaggcg gcgaccgttc
    36121 cagtcgagat ccagctcctg atgaatatga ccgcacagca agtatttgac gtgcggaaac
    36181 ttcgccagca cggtatccag ttcgcccgcg ttacgcagac tgtgttgatc gagccaacta
    36241 caacccgcag gtagcggatg atgatgcagc agcagcaacg tatggcgttc tggcgcatcg
    36301 gccagtttac gttccagcca ctcaagctga aactcgctca gctcaccgtg cggcacgcca
    36361 aacacctggc tatccagcaa caggatttgc cattgctcac caataaacac ccgcttcgcc
    36421 ggggagatac ccgcatcctg taacgcgctg tacatcgcgg gctggaaatc gtggttgccc
    36481 ggcagccaga cgcagggcgc acgaaaactt gcgatgcctt cagcgaaatg ctgataggcc
    36541 gcagaggatt gatcctgcgc taaatcacct gtcgcgacaa tcaggtcgaa ttcgtgctgg
    36601 tgtggacgaa tcgcctccag caccgcctgg tagctttccc aggtgtttac ccctaacagg
    36661 gcttcgtgct tttgtgcaaa caggtgagta tcggtaattt gtaaaatcct tcctctggcc
    36721 tcaccagcca gaggaagggt taacaggctt tccaaatggt gtccttaggt ttcacgacgc
    36781 tagtaaaccg gaatcgccat cgctccatgt gctaaacagt atcgcaacca gtccgctaaa
    36841 aactggttaa tttgatgctt ttcgtcgcgt tgatgcaact ttttattagg ataatcatac
    36901 cgcgctttga agcgaaaaat ctgctggctt gaacacactt cagccaccat cgcgtcatga
    36961 tacagacgca ccgtcattga cggaaggctc cagtaactga tcgcgggcgc agtctgttct
    37021 attgtcacca gggtagtgta tcgggtcgat tccacaatcg tcagccgata ttgtgcgttt
    37081 gccacctgat agcttacagt ttcgccgggt gcgtcattgc gcggtaacaa acggcgcaat
    37141 tgtgaaaagt tcatctcgca caggcgcatc atttcaggaa agtcaggtgt gtaacgcttc
    37201 atttatgccc actcattttt taacgcttga tgatgcagct gcagccattg caaagcgatg
    37261 accgacgctg cgttgtcgat tttcccctct tctacccact ggtatgcctg ttcccggctt
    37321 accacatgaa cgcgaatatc ttcgttttca tcagccagac catgaatacc gcttgcggtc
    37381 gtggcgtcca cttcgcccac cataattgac gaacgctcac tggtgccccc cgggcttgcc
    37441 aggaaactta acaccggttt ggtccgtttg actatcagtc ccgcctcttc aatcgcttcg
    37501 cgacgggcaa catcttccac actttcaccc tcttcaatca tcccggcaac catctccagt
    37561 agccaggggg tttcgctggt gtcgtacgcg gcaatccgaa tctgctcaat cagcacaact
    37621 tcatcacgca ctgggtcaaa gggtagcaag actgcggcgt gaccgcgctc aaaaatttcc
    37681 cgccgtacct catgactcat ttgcccgttg aatagacgat gacgaaatct ataaagatct
    37741 aatgaaaaaa agccgcgata aagtgtttct cgtgcaataa tttctacatc gtttttgcca
    37801 aatgtaacgg gcaggttgtc tggcttaagc attgttaatg tcctggcact aatagtgaat
    37861 taaatgtgaa tttcagcgac gtttgactgc cgtttgagca gtcatatgtt aaattgaggc
    37921 acattaacgc cctatggcac gtaacgccaa ccttttgcgg tagcggcttc tgctagaatc
    37981 cgcaataatt ttacagtttg atcgcgctaa atactgcttc accacaagga atgcaaatga
    38041 agaaattgct ccccattctt atcggcctga gcctttctgg gttcagttcg ttgagccagg
    38101 ccgagaacct gatgcaagtt tatcagcaag cacgccttag taacccggaa ttgcgtaagt
    38161 ctgccgccga tcgtgatgct gcctttgaaa aaattaatga agcgcgcagt ccattactgc
    38221 cacagctagg tttaggtgca gattacacct atagcaacgg ctaccgcgac gcgaacggca
    38281 tcaactcgaa cgcgaccagt gcgtccctgc agttaactca atccattttt gatatgtcga
    38341 aatggcgtgc gttaacgctg caggaaaaag cagcagggat tcaggacgtc acatatcaga
    38401 ccgatcagca aaccttgatc ctcaacaccg cgaccgctta tttcaacgtg ttgaatgcta
    38461 ttgacgttct ttcctataca caggcacaaa aagaagcgat ctaccgtcaa ttagatcaaa
    38521 ccacccaacg ttttaacgtg ggcctggtag cgatcaccga cgtgcagaac gcccgcgcgc
    38581 agtacgatac cgtgctggcg aacgaagtga ccgcacgtaa taaccttgat aacgcggtag
    38641 agcagctgcg ccagatcacc ggtaactact atccggaact ggcggcgctg aatgtcgaaa
    38701 actttaaaac cgacaaacca cagccggtta acgcgctgct gaaagaagcc gaaaaacgca
    38761 acctgtcgct gttacaggca cgcttgagcc aggacctggc gcgcgagcaa attcgccagg
    38821 cgcaggatgg tcacttaccg actctggatt taacggcttc taccgggatt tctgacacct
    38881 cttatagcgg ttcgaaaacc cgtggtgccg ctggtaccca gtatgacgat agcaatatgg
    38941 gccagaacaa agttggcctg agcttctcgc tgccgattta tcagggcgga atggttaact
    39001 cgcagatgaa acaggcacag tacaactttg ttggtgccag tgagcaactg gaaagcgcac
    39061 atcgtagcgt cgtgcaaacc gtacgttcct ccttcaacaa cattaatgct tctatcagta
    39121 gtattaacgc ctacaaacaa gccgtagttt ccgcgcaaag ctcattagac gcgatggaag
    39181 cgggctactc ggtcggtacg cgtaccattg ttgatgtgtt ggatgcgacc accacgctgt
    39241 acaacgccaa gcaagagctg gcgaatgcgc gttataacta cctgattaat cagctgaata
    39301 ttaagtcagc cctgggtacg ttgaacgagc aggatttgct ggcactgaac aatgcgctga
    39361 gcaaaccggt ttccactaat ccggaaaacg ttgccccgca aacgccggaa cagaatgcta
    39421 ttgctgatgg ttatgcgcct gatagcccgg cacccgtcgt tcagcaaaca tccgcacgca
    39481 ctaccaccag taacggtcat aaccctttcc gtaactgatg acgacgacgg ggcttcggcc
    39541 ccgtctgaac gtaaggcaac gtaaagatac gggttatctg ccgcattctt cccccttctc
    39601 gcttcaattt cgaccagcca tcctctattc tgatgggtat ttaccactgg tcccggaaga
    39661 caaaaatgaa acggacaaaa tccatacgcc acgcatcgtt ccgcaaaaac tggagcgcac
    39721 gccatctgac accagtcgct ctcgcggttg ccactgtttt tatgctggct ggctgtgaaa
    39781 agagtgatga aacagtgtct ctctatcaaa atgctgacga ctgttcagct gcaaacccag
    39841 gcaaaagcgc cgaatgtacc accgcgtaca acaatgcgct gaaagaagcc gaacgtactg
    39901 cgccgaaata cgccacccgt gaagactgtg ttgctgaatt tggtgaaggt cagtgtcagc
    39961 aagcaccagc ccaggctggc atggcaccag aaaaccaggc gcaggctcag caatccagcg
    40021 ggagtttctg gatgccgctg atggccggtt acatgatggg gcgtctgatg ggcagcggcg
    40081 cgggatttgc acagcagccg ctgttctcct cgaaaaaccc ggccagtccg gcttacggta
    40141 aatataccga cgcgacgggt aaaaactatg gcgcagccca gccaggccgc accatgaccg
    40201 taccgaagac ggcaatggca ccaaaaccgg cgaccaccac taccgttacc cgtggcggtt
    40261 ttggtgaatc tgttgccaaa caaagcacta tgcagcgtag cgcaaccggc acctcttctc
    40321 gttcaatggg tggctgatac cgatggaaag agtcagtatt accgagcgcc cggactggcg
    40381 tgagaaagcc cacgaatacg gtttcaattt tcacaccatg tacggcgagc cgtactggtg
    40441 tgaagatgct tactacaagt tgaccctcgc ccaggttgaa aagctggaag aagtcaccgc
    40501 cgaactgcac cagatgtgcc tgaaagtggt ggaaaaagtg atcgccagcg atgagctgat
    40561 gaccaaattc cgcataccaa aacacacctg gagttttgtg cgtcaatcat ggctgactca
    40621 ccagccatcg ctttattcgc gtcttgatct ggcgtgggat ggcactggcg aacctaaact
    40681 tctggaaaat aacgccgata cgccaacgtc actatacgag gcggcgttct ttcagtggat
    40741 ctggctggaa gatcagctta acgccggtaa cttgccggag ggcagcgacc agtttaacag
    40801 tctgcaagaa aaactgatcg atcgcttcgt tgagctgcgt gaacagtatg gcttccagtt
    40861 gctgcatctc acctgctgtc gcgacacggt ggaagatcgc ggaaccattc agtatttgca
    40921 ggactgcgca accgaagctg aaatcgccac cgagttcctc tacatcgacg agataggttt
    40981 aggcgaaaaa ggccagttca cggatttaca ggatcaggtg atttctaacc tgttcaagct
    41041 ttatccgtgg gaatttatgc tgcgcgaaat gttctccacc aagctggaag atgctggcgt
    41101 acgctggctg gaaccggcgt ggaagagcat tatctccaac aaggcacttc taccgctact
    41161 gtgggagatg ttcccgaatc acccgaacct gctgcccgct tattttgcgg aagatgatca
    41221 tccgcaaatg gaaaaatatg tggttaaacc gatcttctcc cgtgaaggcg caaacgtgtc
    41281 gatcattgag aacggcaaaa ccattgaagc agcggaaggt ccgtatggcg aagaagggat
    41341 gattgttcag caattccacc cgttaccgaa attcggcgac agctatatgc tgattggtag
    41401 ctggctggtg aacgatcaac ctgccggaat tggcattcgt gaagaccgtg cgttgatcac
    41461 ccaagatatg tctcggtttt atccacatat ttttgttgaa taagccacga taccggatgg
    41521 cactcgccat ccggtaattg ttagcctatc tgcaccgaca gcatacttaa actgcccatt
    41581 tctataccat cgaccggaat ggtaattggc tcctgcccat cccacacacc taacacatac
    41641 aacaacggca gatagtgctc tggcgttggg ttcgataacg cgccaccttc atggtcgaga
    41701 taattcacca gaggatgctg ctctaccggc ccttgccacg tcagattcgc tttcacatac
    41761 tcattaaacg acgtcgccca cggatacggt gaactatcac cgtgccactt cactgtgcgc
    41821 aggttatgca ccacgttacc gctggcgacc aacattattc cttcatcacg cagcgccgcg
    41881 agtttgcgcc ccatttcgaa atgccaggcg gcaggtttgc tactgtcgat actcaactgc
    41941 accataggga tatcggcgtc aggatacatc ttaatcagca cgccccacga gccgtggtca
    42001 aagccccagg cttctttatc cagcgtcacc gggatcggcg ctaacagctc aaccagacgc
    42061 tgtgccagcg caggcgaacc cggagcagga taatgcgtat cgtacagcgc ctgcgggaag
    42121 ccaccaaagt catgaatcgt gggcggcgtc tccatcgcgg tcactcctgt tccacgggta
    42181 aaccagtgag ccgaaaccac cacaatcgct tgcgggcgtg gcaatgtcat ccccaacttc
    42241 tgccagctgc gggtatacaa attatcttcc agcacgttca tcggactacc gtgacctaaa
    42301 aacaatgctg gcatacgtgt tgaagacatg atgatatcct taactaaagg tgtcattttg
    42361 atatcctcac aatacgcttg ttcggcggag taagaacccg gataacaatg atgatgatca
    42421 tcagttattt tgacgatctg cctgaaggtg aagatttata aggagttgtc gatgtcagta
    42481 cctctcattc tgaccatact ggcgggggca gccacgttta ttggcgcgtt tctcggcgtt
    42541 ctcggacaaa aaccctcgaa ccgcttactg gcgttttcgc taggttttgc ggcggggatc
    42601 atgttgctca tctcattaat ggaaatgctt cctgccgcac tagcagctga aggaatgtcg
    42661 ccggtgttgg gttatggaat gtttatcttc ggtctgcttg gctactttgg cctggaccgc
    42721 atgttgccgc atgcgcatcc gcaggattta atgcaaaaat cggtgcagcc gttgccaaaa
    42781 tcgatcaagc gcacagccat tctgctcact ctcggcatca gtctgcataa cttcccggaa
    42841 gggattgcca cctttgtcac ggcgagcagc aacctggagc tgggatttgg catcgcactg
    42901 gccgtcgcgt tgcacaatat ccctgaaggt ctggcagtgg caggcccggt ttatgcggca
    42961 acggggtcta aacgcaccgc gattctgtgg gcggggattt ctggactagc agaaattctt
    43021 ggtggtgtgc tggcgtggtt aatcctcggt agcatgattt ccccggtggt catggcggca
    43081 atcatggcgg cggttgcagg aattatggtg gcgctctcgg ttgatgaatt aatgccgctc
    43141 gccaaagaga ttgaccctaa taataacccc agctatggcg tactatgtgg aatgtcagtg
    43201 atgggattca gtttagtgct gctacaaacg gcgggaattg gttaaaaaca acatcagaag
    43261 ttgtccagca catggacaac ttctgccagc ttcacgaaag ccttattcta ccttttcggc
    43321 tgtctcttct ctcgtactgt ttaagtattt gttccgcgct tcaagcagtg cgtctctgtc
    43381 ctggcgaaac gcccgacaat aacgtttcaa atgcaggaaa taacccacca caaccagagc
    43441 aatcaataca atccagtacc aggcgataaa catcctttta cccttaatat ttaacaatac
    43501 taaacattta gcgcataaat ttcacatatc ctttttcgga tatattcata tggtcgcata
    43561 ataacggaac aattatatga ttttgatcat aatgaaaaag aatgcctgat attaatgaaa
    43621 ttttctcata gaaagagaag cggaattaac atcttgcata gcagggcaaa ataaaaaccg
    43681 aagccagcta caacttcggt ttcttgatta aggcagtaat taagcagcgg ttttcagctg
    43741 gctttacgct catgtgcctg acggtatgcc accaggtctt caatagtcac gagcgccata
    43801 ttgtgtttat tggcaaactc aatgcactct ggtgcacgcg ccatcgtgcc atcgtcatta
    43861 gtcagctcac acagcacacc agccggttta aagcctgcca gcgtcatcaa atcaatagtc
    43921 gcttcagtat gaccgccacg cgtcagtaca ccacctgcct gcgcgcgaag tgggaaaacg
    43981 tggcccgggc gattcagatc agaaggtttt gcgccatcgg caatcgctgc gcgaacggtc
    44041 gtaatacgat cagcggcaga aacaccggta gtcacacctt cagccgcttc aatggtcacg
    44101 gtaaaaccgg tgccataggc gctggtgtta ttttctacca tcattggcag atcaagttgt
    44161 ttacggcgat cttcagtgat gcacaggcaa acaataccgc taccgtggcg aatggtcagc
    44221 gccatctgct caacggtcat ggtttctgcc gggaagatca tatcgccttc gttttcacgg
    44281 tcttcatcat caagcaccat tacaccgcgt ccttcacgca gcgcagccag tgcattttca
    44341 acacgttcga aaggcgtacc aaaagaggaa agtagcgtct gattcatggt aaaaaaacct
    44401 cactaaaatt atggttacca gaatcagggc agtcttagga gtggcggcat atagccaaaa
    44461 taacgtgagc gggtccatgc ccgacagaat cgttactctc tcccatccgg actctaaccg
    44521 tcggccccgg aattacaccg gatctgctgt cctttgagtt cgcacccaaa gcgctcgcgg
    44581 gctttcaact gagttgattt accgccggtg gggaatttcg ccccgccctg agaataagcg
    44641 gattcactat aacgctaatg attagcggca gcaacgcata gcttcacata attctggttt
    44701 atgacttacc cttatcgcac tacaatggta ctcaacacct atcagtacag ggaaactaca
    44761 atgattgacc cgaaaaaaat tgagcaaatc gctcgccagg ttcacgaatc aatgcctaaa
    44821 ggaatcaggg agttcgggga agatgtggag aaaaaaatcc gccaaaccct acaagctcag
    44881 ctgacgcgcc tcgaccttgt aagccgtgaa gagttcgacg tccaaacgca agtcctgtta
    44941 cggactcgtg agaaactggc gctgcttgaa caaagaatca gcgaactgga aaatcgcagt
    45001 actgagatca aaaagcagcc agatccagag actctccccc caacgctgta aacatttgtc
    45061 tttaatgcac tgtaaattat gaattaatcc tctaatttac agtgttttct attacattat
    45121 cgcttaatcc cattcagagt atcatcacaa aaatgaatga ttgcaattga aaatataatt
    45181 gttatgtatt ctttaagttt tacttaatcg atatttagac tggccccctg aatctccaga
    45241 caaccagtat cacttattta agtgatagtc tt
//
LOCUS       DBKEKI010000020        43595 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.020, whole genome shotgun sequence.
ACCESSION   DBKEKI010000020 DBKEKI010000000
VERSION     DBKEKI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.020"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..82)
                     /locus_tag="ACUQPX_001039"
     CDS             complement(<1..82)
                     /locus_tag="ACUQPX_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HHV9055813.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQL"
     gene            complement(105..536)
                     /locus_tag="ACUQPX_001040"
     CDS             complement(105..536)
                     /locus_tag="ACUQPX_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HHV9055814.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            908..1927
                     /gene="epd"
                     /locus_tag="ACUQPX_001041"
     CDS             908..1927
                     /gene="epd"
                     /locus_tag="ACUQPX_001041"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HHV9055815.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            1977..3140
                     /gene="pgk"
                     /locus_tag="ACUQPX_001042"
     CDS             1977..3140
                     /gene="pgk"
                     /locus_tag="ACUQPX_001042"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HHV9055816.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            3355..4434
                     /gene="fbaA"
                     /locus_tag="ACUQPX_001043"
     CDS             3355..4434
                     /gene="fbaA"
                     /locus_tag="ACUQPX_001043"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HHV9055817.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            4625..5485
                     /gene="mscS"
                     /locus_tag="ACUQPX_001044"
     CDS             4625..5485
                     /gene="mscS"
                     /locus_tag="ACUQPX_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HHV9055818.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            5624..6259
                     /gene="argO"
                     /locus_tag="ACUQPX_001045"
     CDS             5624..6259
                     /gene="argO"
                     /locus_tag="ACUQPX_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HHV9055819.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            6352..7092
                     /locus_tag="ACUQPX_001046"
     CDS             6352..7092
                     /locus_tag="ACUQPX_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HHV9055820.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            7259..8155
                     /gene="srsR"
                     /locus_tag="ACUQPX_001047"
                     /gene_synonym="ygfI"
     CDS             7259..8155
                     /gene="srsR"
                     /locus_tag="ACUQPX_001047"
                     /gene_synonym="ygfI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator SrsR"
                     /protein_id="HHV9055821.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(8152..9630)
                     /gene="scpC"
                     /locus_tag="ACUQPX_001048"
     CDS             complement(8152..9630)
                     /gene="scpC"
                     /locus_tag="ACUQPX_001048"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HHV9055822.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYNNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(9654..10439)
                     /gene="scpB"
                     /locus_tag="ACUQPX_001049"
     CDS             complement(9654..10439)
                     /gene="scpB"
                     /locus_tag="ACUQPX_001049"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HHV9055823.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDNLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(10450..11445)
                     /gene="meaB"
                     /locus_tag="ACUQPX_001050"
     CDS             complement(10450..11445)
                     /gene="meaB"
                     /locus_tag="ACUQPX_001050"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HHV9055824.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMMLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDSDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSME
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(11438..13577)
                     /gene="scpA"
                     /locus_tag="ACUQPX_001051"
                     /pseudo
     CDS             complement(11438..13577)
                     /gene="scpA"
                     /locus_tag="ACUQPX_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(13781..14674)
                     /gene="argP"
                     /locus_tag="ACUQPX_001052"
     CDS             complement(13781..14674)
                     /gene="argP"
                     /locus_tag="ACUQPX_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HHV9055825.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            15057..15716
                     /gene="rpiA"
                     /locus_tag="ACUQPX_001053"
     CDS             15057..15716
                     /gene="rpiA"
                     /locus_tag="ACUQPX_001053"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HHV9055826.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            15972..17204
                     /gene="serA"
                     /locus_tag="ACUQPX_001054"
     CDS             15972..17204
                     /gene="serA"
                     /locus_tag="ACUQPX_001054"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HHV9055827.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            17433..17492
                     /gene="ibsC"
                     /locus_tag="ACUQPX_001055"
     CDS             17433..17492
                     /gene="ibsC"
                     /locus_tag="ACUQPX_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HHV9055828.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            17825..17884
                     /locus_tag="ACUQPX_001056"
     CDS             17825..17884
                     /locus_tag="ACUQPX_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HHV9055829.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(17985..18587)
                     /locus_tag="ACUQPX_001057"
     CDS             complement(17985..18587)
                     /locus_tag="ACUQPX_001057"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HHV9055830.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(18608..18791)
                     /gene="ssrS"
                     /locus_tag="ACUQPX_001058"
     ncRNA           complement(18608..18791)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACUQPX_001058"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(18833..19162)
                     /gene="zapA"
                     /locus_tag="ACUQPX_001059"
     CDS             complement(18833..19162)
                     /gene="zapA"
                     /locus_tag="ACUQPX_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HHV9055831.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            19330..19908
                     /gene="ygfB"
                     /locus_tag="ACUQPX_001060"
     CDS             19330..19908
                     /gene="ygfB"
                     /locus_tag="ACUQPX_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HHV9055832.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            19934..21259
                     /gene="pepP"
                     /locus_tag="ACUQPX_001061"
     CDS             19934..21259
                     /gene="pepP"
                     /locus_tag="ACUQPX_001061"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HHV9055833.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQDEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            21256..22434
                     /gene="ubiH"
                     /locus_tag="ACUQPX_001062"
                     /gene_synonym="visB"
     CDS             21256..22434
                     /gene="ubiH"
                     /locus_tag="ACUQPX_001062"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HHV9055834.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLVAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            22457..23659
                     /gene="ubiI"
                     /locus_tag="ACUQPX_001063"
                     /gene_synonym="visC"
     CDS             22457..23659
                     /gene="ubiI"
                     /locus_tag="ACUQPX_001063"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HHV9055835.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(23733..23816)
                     /locus_tag="ACUQPX_001064"
     CDS             complement(23733..23816)
                     /locus_tag="ACUQPX_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055836.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            24107..25201
                     /gene="gcvT"
                     /locus_tag="ACUQPX_001065"
     CDS             24107..25201
                     /gene="gcvT"
                     /locus_tag="ACUQPX_001065"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HHV9055837.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            25225..25614
                     /gene="gcvH"
                     /locus_tag="ACUQPX_001066"
     CDS             25225..25614
                     /gene="gcvH"
                     /locus_tag="ACUQPX_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HHV9055838.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            25733..28606
                     /gene="gcvP"
                     /locus_tag="ACUQPX_001067"
     CDS             25733..28606
                     /gene="gcvP"
                     /locus_tag="ACUQPX_001067"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HHV9055839.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            28873..29616
                     /locus_tag="ACUQPX_001068"
     CDS             28873..29616
                     /locus_tag="ACUQPX_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHV9055840.1"
                     /translation="MAIALVTGGSCGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(29673..31106)
                     /gene="bglA"
                     /locus_tag="ACUQPX_001069"
     CDS             complement(29673..31106)
                     /gene="bglA"
                     /locus_tag="ACUQPX_001069"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HHV9055841.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMCFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            31151..31462
                     /gene="yqfB"
                     /locus_tag="ACUQPX_001070"
     CDS             31151..31462
                     /gene="yqfB"
                     /locus_tag="ACUQPX_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HHV9055842.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            31626..32285
                     /gene="trhA"
                     /locus_tag="ACUQPX_001071"
     CDS             31626..32285
                     /gene="trhA"
                     /locus_tag="ACUQPX_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HHV9055843.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(32481..33461)
                     /gene="ygfZ"
                     /locus_tag="ACUQPX_001072"
     CDS             complement(32481..33461)
                     /gene="ygfZ"
                     /locus_tag="ACUQPX_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HHV9055844.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTALAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            33704..33970
                     /gene="sdhE"
                     /locus_tag="ACUQPX_001073"
     CDS             33704..33970
                     /gene="sdhE"
                     /locus_tag="ACUQPX_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HHV9055845.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            33951..34358
                     /locus_tag="ACUQPX_001074"
     CDS             33951..34358
                     /locus_tag="ACUQPX_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HHV9055846.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(34398..34919)
                     /gene="fldB"
                     /locus_tag="ACUQPX_001075"
     CDS             complement(34398..34919)
                     /gene="fldB"
                     /locus_tag="ACUQPX_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HHV9055847.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            35031..35927
                     /gene="xerD"
                     /locus_tag="ACUQPX_001076"
     CDS             35031..35927
                     /gene="xerD"
                     /locus_tag="ACUQPX_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HHV9055848.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            35952..36662
                     /gene="dsbC"
                     /locus_tag="ACUQPX_001077"
     CDS             35952..36662
                     /gene="dsbC"
                     /locus_tag="ACUQPX_001077"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HHV9055849.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            36668..38401
                     /gene="recJ"
                     /locus_tag="ACUQPX_001078"
     CDS             36668..38401
                     /gene="recJ"
                     /locus_tag="ACUQPX_001078"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HHV9055850.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            38492..39590
                     /gene="prfB"
                     /locus_tag="ACUQPX_001079"
     CDS             join(38492..38566,38568..39590)
                     /gene="prfB"
                     /locus_tag="ACUQPX_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HHV9055851.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            39600..41117
                     /gene="lysS"
                     /locus_tag="ACUQPX_001080"
     CDS             39600..41117
                     /gene="lysS"
                     /locus_tag="ACUQPX_001080"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HHV9055852.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFITLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(41160..41708)
                     /gene="idi"
                     /locus_tag="ACUQPX_001081"
     CDS             complement(41160..41708)
                     /gene="idi"
                     /locus_tag="ACUQPX_001081"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HHV9055853.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            41763..41834
                     /locus_tag="ACUQPX_001082"
     CDS             41763..41834
                     /locus_tag="ACUQPX_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055854.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(41831..41956)
                     /gene="yqfG"
                     /locus_tag="ACUQPX_001083"
     CDS             complement(41831..41956)
                     /gene="yqfG"
                     /locus_tag="ACUQPX_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HHV9055855.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(41958..43406)
                     /gene="uacT"
                     /locus_tag="ACUQPX_001084"
     CDS             complement(41958..43406)
                     /gene="uacT"
                     /locus_tag="ACUQPX_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HHV9055856.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESISDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        10539 a        10924 c        11636 g        10496 t
ORIGIN      
        1 ccagttgccc gccgatcata ttgatggcga tagatataac gatcaacacc agtgcctgac
       61 tggaaaagtg gctacgcgcc ataagaaaac ctctttgaaa gtagttattt aacgggggaa
      121 atatgcaact cgtcgtaacc agaaagcgcg caaggttggc ggctaaacgc cagttcctca
      181 aggctaccga tcaccagttc aagcgtggtc cgcacggtac agccgggcat catatgaccg
      241 ccaagcatcg tgccgtgtgg atcggaaacg cagagatgaa gatgctcacc actctgttcc
      301 agcgtgccgt ttagtgagat cacttcgaat gtaccgttca ggagcgtggt gccttcttgc
      361 ccggcatagc gcaaggcgac gtcagtcagg ctaccggtac agcctgcaat ccaggcggca
      421 tgaagttgat gttgctgtgc aaaagcgtgt agctgggaaa aaacttcctg cccggggagg
      481 aggcgcagtg cgtaaaagcg tgcggaggaa gaatatgggt agggcaatgt catcattgga
      541 gtactccgaa actcggacgt attgcggata atatcgacgc tgcatcagtc atattctgat
      601 ataagtcagg gtttgtgacg gcacaacgaa aagattttgc tacgcttatt cgtgattctg
      661 caaagtggaa taaattccac aggggcgggg ctgaaaacgt gacgtaagtc agataattgc
      721 tttctttgac tggacaatca ttccttttat tccacgtttc gcttatccta gctgaagcgt
      781 ttcagtcgat taaatgttcg acaattaacc aatcagtcgc agtttgcgac aggtaaggtt
      841 tccccggacg atttgctgga ttactctgtc tggcaaattt gctcagggaa aaccttgcag
      901 gagatctatg accgtacgcg tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt
      961 tcgtgctttg tatgaatccg gacgccgggc ggaaattacc gtggtggcaa tcaacgaact
     1021 ggcggatgct gcgggcatgg cgcatttgtt gaaatatgac accagccatg gccgttttgc
     1081 atgggaagta cgacaggaac gcgatcaact ttttgttggt gatgacgcca tccgcgtatt
     1141 gcatgaacgt tcactgcaat cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga
     1201 ctgcaccggc gtatatggct cccgcgagca tggcgaagcg catattgccg ccggggccaa
     1261 aaaagtgctc ttttcacatc ctggcagtaa cgatctcgac gcgaccgttg tttacggcgt
     1321 caatcaggat caacttcgtg cggaacaccg catcgtttct aacgcttcct gtaccacgaa
     1381 ttgcataatt cccgtcatca aattgttaga tgatgcgtac ggtattgagt ccggcactgt
     1441 gaccacaatt cactccgcca tgcacgatca acaggttatt gatgcatacc atcctgacct
     1501 gcgtcgcacc cgggcagcca gccagtcgat cattccggtc gatactaaac tggccgctgg
     1561 tatcacacga ttttttccgc aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc
     1621 aaccataaat gtgacggcaa tcgatttaag cgtgacggtg aagaaacctg taaaagccaa
     1681 tgaagtcaac ctgttgctgc aaaaagcagc acaaggtgca tttcatggta tagttgacta
     1741 tacggaattg ccgttggtct ctgtagattt taaccacgat ccgcacagtg ccattgtcga
     1801 tggcacccaa acccgggtca gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa
     1861 cgaatggggc tttgctaacc gaatgctcga cacgacgtta gctatggcta ctgttgcttt
     1921 caggtaagac gcaagcagcg tctgcaaaac ttttagaatc aacgagagga ttcaccatgt
     1981 ctgtaattaa gatgaccgat ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc
     2041 tgaacgtacc agtaaaagac gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc
     2101 cgaccatcga actggccctg aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc
     2161 gtcctaccga aggcgagtac aacgaagaat tctctctgct gccggttgtt aactacctga
     2221 aagacaaact gtctaacccg gttcgtctgg ttaaagatta cctcgacggc gttgacgttg
     2281 ctgaaggtga actggttgtt ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg
     2341 acgaaaccct gtccaaaaaa tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg
     2401 gtactgctca ccgcgcgcag gcttctactc acggtatcgg taaattcgct gacgttgcgt
     2461 gcgcaggccc gctgctggca gctgaactgg acgcgctggg taaagcactg aaagaacctg
     2521 ctcgcccgat ggtggctatc gttggtggtt ctaaagtatc taccaaactg accgttctgg
     2581 actccctgtc taaaatcgct gaccagctga ttgttggtgg tggtatcgct aacaccttta
     2641 tcgcggcaca aggccacgat gtgggtaaat ccctgtacga agctgacctg gttgacgaag
     2701 ctaaacgtct gctgaccacc tgcaacatcc cggttccgtc tgatgttcgc gtagcaaccg
     2761 agttctctga aactgcaccg gctaccctga aatctgttaa cgatgtgaaa gctgacgagc
     2821 agatcctgga tatcggtgat gcttccgctc aggaactggc tgaaatcctg aagaatgcga
     2881 aaaccattct gtggaacggt ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta
     2941 ctgaaatcgt ggctaacgct atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg
     3001 acactctggc agcaatcgac ctgttcggca ttgctgacaa aatctcctac atctccactg
     3061 gcggcggcgc attcctcgaa ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg
     3121 aagagcgcgc taagaagtaa aaaatcacag ggcagggaaa cctgcccttg tttcagcgcg
     3181 cttttagagc acgcattttt tcatactcta aataattcga gttgcaggaa ggcgacaaga
     3241 gagtgaatcc ccaggagctt acataagtaa gtgactgggg tgagcgaacg cagacgcagc
     3301 acatgcaact tgaagtatta tgagtataag gcccgacgat acaggacaag agacatgtct
     3361 aagatttttg atttcgtaaa acctggcgta atcactggtg atgacgtaca gaaagttttc
     3421 caggtagcaa aagaaaacaa cttcgcactg ccagcagtaa actgcgtcgg tactgactcc
     3481 atcaacgccg tactggaaac tgctgctaaa gttaaagcgc cggttatcgt tcagttctcc
     3541 aacggtggtg cttcctttat cgctggtaaa ggcgtgaaat ctgacgttcc gcagggtgct
     3601 gctatcctgg gcgcgatctc tggtgcgcat cacgttcacc agatggctga acattatggt
     3661 gttccggtta tcctgcacac tgaccactgc gcgaagaaac tgctgccgtg gatcgacggt
     3721 ctgctggacg cgggtgaaaa acacttcgca gctaccggta agccgctgtt ctcttctcac
     3781 atgatcgacc tgtctgaaga atctctgcaa gagaacatcg aaatctgctc taaatacctg
     3841 gagcgtatgt ccaaaatcgg catgactctg gaaatcgaac tgggttgcac cggtggtgaa
     3901 gaagacggcg tggacaacag ccacatggac gcttctgcac tgtacaccca gccggaagac
     3961 gttgattacg catacaccga gctgagcaaa atcagcccgc gtttcaccat cgctgcgtcc
     4021 ttcggtaacg tacacggtgt ttacaagccg ggtaacgtgg ttctgactcc gaccatcctg
     4081 cgtgattctc aggaatatgt ttctaagaaa cacaacctgc cgcacaacag cctgaacttc
     4141 gtattccacg gtggttccgg ttctactgct caggaaatca aagactccgt aagctacggc
     4201 gtagtgaaaa tgaatatcga taccgatacc caatgggcaa cctgggaagg cgttctgaac
     4261 tactacaaag cgaacgaagc ttatctgcag ggtcagctgg gtaacccgaa aggcgaagat
     4321 cagccgaaca agaaatacta cgatccgcgc gtatggctgc gtgccggtca gacttcgatg
     4381 atcgctcgtc tggaaaaagc attccaggaa ctgaacgcga tcgacgttct gtaagtcatt
     4441 cctttctgct tatctcaagg cccgctctgc gggtcttttt ttcgccaaaa gcaaggtgat
     4501 tcagatgaga aatctgtgat ctatttggca aaattatgct ttattgttta cccttgtcag
     4561 actgcccgtc ataaggcggc ggagtgtatt tctccatttt gagtcagttg aaaaggaata
     4621 ttgaatggaa gatttgaatg ttgtcgatag cataaacggc gcgggaagct ggctggtagc
     4681 taaccaggcg ctgctgctaa gttatgcagt aaacatcgtg gcggcactcg cgatcatcat
     4741 cgttggtttg attatcgcgc ggatgatttc caacgcggtg aatcgcctga tgatctcccg
     4801 taaaatcgat gccactgttg ctgattttct ttctgcatta gtccgttacg gtattatcgc
     4861 ctttacgcta atcgctgccc tgggacgcgt gggtgtacaa acagcgtcag tcattgctgt
     4921 actcggtgcc gcaggcttag ctgttggtct ggctttgcag gggtcacttt ctaacctggc
     4981 cgctggcgtg ttacttgtca tgttccgccc gttccgtgcc ggagaatatg ttgacctcgg
     5041 cggcgtagcc ggtactgtgc tgagtgtgca gattttctcc accaccatgc gtactgcaga
     5101 cggtaaaatt atcgttattc cgaacggtaa aattattgcc ggaaatatta ttaacttctc
     5161 ccgcgagcca gttcgccgta acgaatttat tattggcgtg gcgtatgatt ccgatatcga
     5221 tcaggttaag cagatcctga ccaatattat ccagtctgaa gatcgcattt tgaaagatcg
     5281 cgaaatgact gtgcgcctga acgaacttgg tgcatcgtcg attaatttcg tggtccgcgt
     5341 ctggagcaac agcggcgatc tgcaaaacgt gtactgggat gtgctggagc gtattaaacg
     5401 tgaatttgat gccgccggta tcagcttccc gtacccgcaa atggatgtga actttaagcg
     5461 ggtgaaagaa gacaaagctg cgtaatcaac gctgaggcca gataatactc tctggcctct
     5521 ctcttattag ttattctgat tgccaattaa tattatcaat ttccgctaat aacaatcccg
     5581 cgatatagtc tctgcatcag atacttaatt cggaatatcc aacgtgtttt cttattactt
     5641 tcaaggtctt gctcttgggg cagctatgat tctgccgctc ggcccacaaa atgcttttgt
     5701 gatgaatcag ggcattcgtc gtcagtacca cattatgatt gccttacttt gcgctatcag
     5761 cgatttggtc ctgatttgcg cggggatttt tggtggtagc gcgttattga tgcagtcgcc
     5821 gtggttgctg gcgctggtca cctggggcgg cgtagtcttc ttgctctggt atggttttgg
     5881 cgcttttaaa acagcgatga gtagtaatat tgagttagct agtgccgaag tcctgaagca
     5941 aggcagatgg aaaattatcg ccaccatgtt ggcagtgacc tggctgaatc cgcatgttta
     6001 cctggatact tttgttgtac tgggcagcct tggcgggcag cttgacgtcg aaccaaaacg
     6061 ttggtttgcg ctcgggacaa ttagcgcctc tttcctgtgg ttctttggtc tggctattct
     6121 cgcagcctgg ctggcaccgc gcctgcgcac ggcaaaatca cagcgcatta tcaatctggt
     6181 agtgggatgt gtcatgtggt ttatagcctt gcagctggcg agagacggta ttgctcatgc
     6241 acaagccttg ttcagttagg cgctatctga tggaaaaata aaacagaggc gctaagcttg
     6301 cctccagagg tcctgaattt tgggcaagtc aattaacacg gagagactaa cgtgaagttc
     6361 aaagttatcg ccctggcggc attaatgggt attagcggga tggcagcgca ggctaacgaa
     6421 ttgccggatg gaccgcatat tgtcacctcc ggtacggcaa gtgtggatgc ggtgccagac
     6481 attgccactc ttgcgattga agttaacgtg gccgcgaagg atgccgctac agccaagaaa
     6541 caggcagatg agcgcgtcgc acaatacatt tccttccttg aactcaatca gatcgcgaaa
     6601 aaagatatca gctcagcgaa tttacgcacc cagccagatt atgactatca ggatggtaaa
     6661 agtatcctga aaggctaccg agctgtgaga acggtggaag tcacgctccg tcagttagac
     6721 aaactgaatt ccttgctgga tggcgcgctg aaggcgggtc ttaacgaaat tcgttctgtg
     6781 tcgctgggcg tggcgcagcc ggatgcctat aaagacaaag cgcgtaaggc agcgattgat
     6841 aacgcgattc atcaggcgca ggaactggcg aacggctttc atcgtaaact ggggccggta
     6901 tatagcgtgc gctaccatgt ttccaactat cagcccagcc caatggtgcg gatgatgaaa
     6961 gccgatgccg cgccggtgtc tgcccaggaa acttacgagc aggccgctat tcagtttgat
     7021 gatcaggtcg atgtggtctt ccagttagaa cctgtggatc aacaacccgc taaaacacct
     7081 gcagcacaat aatacttgta gcccgacgta ttcgtcgggc taatgccttt acccgatatt
     7141 gcctcctttt tctgtaattt tctgtaaaac catgacgtaa tcacctatct ttgctcgcgg
     7201 tttcatcaag gataggctga ctgaaacggt taagcggaca aggatgcggg gaggggggat
     7261 ggacattttt atttcgaaaa agatgcgcaa tttcatttta ttagcgcaaa ccaataatat
     7321 tgcccgggcg gcagaaaaaa tccatatgac ggcttcgcca tttggtaaaa gcattgccgc
     7381 gctggaagaa caaattggct atacgctatt tacccgcaaa gataataata tcagcctcaa
     7441 taaagccggt caggaactgt atcaaaaact gttcccggtt tatcaacgac tttctgcgat
     7501 tgataatgaa atccataact cagggcgtcg ttcacgagaa attgtgattg gcatagataa
     7561 tacttatccg acaattattt tcgatcagtt gattagtctc ggagataagt atgaaggcgt
     7621 gaccgcccag ccagttgagt tcagcgaaaa cggcgttatc gataatcttt ttgaccgtca
     7681 gttagatttt attatctccc cacagcatgt atctgcacga gttcaggaac tggaaaacct
     7741 gactatcagt gaattaccac cgctgcgatt gggctttctc gtttctcgcc gttatgaaga
     7801 gcgtcaagag caagagctgc ttcaggagct gccctggcta caaatgcgct ttcaaaaccg
     7861 ggctaacttc gaagcaatga ttgatgcgaa tatgcgacct tgtgggatca atcccaccat
     7921 tatttatcgt ccctacagtt ttatggccaa aatcagcgcc gtggagcgcg gacattttct
     7981 gacggtgatc ccgcattttg cctggcgact ggtgaatccg gcaacgctga aatattttga
     8041 tgcgccgcac aggccgatgt atatgcagga atatctttat tcaatcagaa atcatcgtta
     8101 taccgccacg atgcttcagc atattgctga agatcgtgac gggacaaacc attaacccag
     8161 catcgagccg gttgcaatta aattacggtg taagtcgaag acgtggctaa gatcgtggtg
     8221 aatatgtccg ccgggcgcat tttccagata gcgatgcaga taatcccgat acataggatg
     8281 tgcacaatta tcaatgatag tgcgggcgcg ttgaagcggg gaaagaccgc gcagatcggc
     8341 gatcccttgt tcggtgatga tcactttgac gctgtgttcg ctgtgatcaa catggctgca
     8401 cattggcacg acggttgaga tcttgccttc tttagcaatc gacggagcca taaagatcga
     8461 cagatacgcg ttgcgttcaa aatcaccgct gccgccgatg ccgttcatca gatcgacccc
     8521 ggctacgtgc gttgaatttg catgcccgta aatatcaaac tccaggccga cgttcagagc
     8581 gatgacgccc agacgacgga tgatttccgg gttattggaa atctcctgcg gacgcaacac
     8641 aatgcggctg gcaaagtaat ccatattgtt gtaaatcttg cgcagggaat cggccgagat
     8701 tgtcaggctg gaggcgctgg ccccgctgat tttgccggtt tccagtaaat gcaccaccga
     8761 ttcctgtagc acttccgaat acatcatgaa cggaggaatt tccgggtttt cccccagacg
     8821 cgccattacc gcattattga tattgcccac gccactttgc agcggcagaa attccggcgg
     8881 aatacgccca tgcgccattt cctgcaataa gaacgtgacc acgttatcgg caatctgctg
     8941 gcacatggga ttttgcttat ccagcatatt accggcgtcg ggcaagttgg tttccacgac
     9001 ggcgacaatc tttttcggat cgatttgcac atagcgggta ccgacgcgat ccattgcatg
     9061 gaagatcgac acgctattgc gccgcggtgg cgcgccagga atcacaatat ctgccagttc
     9121 tgcaacgcgc ggatcgtgat agtgattaag ttcaatgatc actttcttcg ctcgcagcag
     9181 ccaggtcggc gcattaccga tcccgctggt taaccagact cgaccatccg gtgccagtgc
     9241 cgatgcttca atgacggcaa catcaatgtc gccgaagaaa ccgtaattga ccatttgcgc
     9301 cacttcgctc aaatgcaggt caacgaaact caccgcgccc tgattgatct ttttacgtaa
     9361 actggacgat gtttgatatg gcgcacgcca ggaaacagca tcggcgtcag aaagtacatc
     9421 gtcagcggcg gcgctgattg acgcccccgt cagcaggcga atttgatacg gctttttggc
     9481 ctcatgctgt tcgttagctc tgcgggcaat cgcggcgggt agggctttcg gcgaacccgc
     9541 tggggtaaag ccgctaaatg ccaccatgtc gttatgctgg ataatttctg ccgcttcatc
     9601 ggcggtcatc cttgtccact gagtttccat ttttactcct tcgttcgcag ggattaatga
     9661 ccaacgaaat taggtttacg tttttcgagg aaagcgttca tcccttcctg gtaatcttcg
     9721 ctgtcataaa ccgcgcggcg catcccctga atacgttcaa attcatcgga gttcatggtg
     9781 tgtgcttcgc ccagtacacg cagctcttct ttgataacgg caatggctaa cggcgctttc
     9841 tcagagatgt ggtgcgccat ttgtaaggtg aaatcttcca gttcttccac ttccacaaca
     9901 tggttgagga tgccgacagc cagcgcgcgc tgggcggtga ttggcgaagc ggtaaaaatc
     9961 agctctttga caatgtggaa gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta
    10021 tacgggacgc cgaggtttac aggcgtcatt gagaaggttg aggtactggc ggcgatgatc
    10081 agatcggaac tcatgatcat ttcaaatgcg ccaccccaaa cactaccttc caccatcgaa
    10141 atgatcggtt tcgggaattt ttggatcatg cgggtgattt gacgcaatgg atcatcatag
    10201 gagagcggat cgcgaccgcc agacggcagt tcgtgaatat cgtgacctgc ggagaagact
    10261 ttggatccac ttggcgcccg caaaatgata cagcgaattt ccggccggtt gagatcgctt
    10321 aacgcctgca taagattatc aataaagact ttacttaagg cattaagttt tcgtccatag
    10381 ttaaactcaa tgaccgccac tttgttgata gtggcaacgt tcacatactg ataagacata
    10441 aaaattcctt taatcaaaat attgcgtctg gataaattca ctgagctgcc gcaggccggt
    10501 gcgcggtgag agcgtattgt ttttgactgc taaaagcgtc tggcggtaat agcgatcgaa
    10561 atcttcattc gcgaacaggt gattcagtac ttcttcttcg gtctgcttac gcagccattc
    10621 catcgattgt tgttgccgca cttgttgtaa acgaccactg gcagttagcg cggttttgaa
    10681 gtcgatgatg gcgtgccaga tctcatcgat tccacgtttt tccagtgcgc tacaagtcag
    10741 accccgtggc tgccattcgt cgtatttacg tcgcagaata tgcagggcac tctcgtacat
    10801 atgccgggca atggcgacat tggtatggtt atcgctatcg tctttgttga taacgagcag
    10861 atcagccact tccatcagcc cttttttaat gccctgcaga tcatcgccgc caccggcaat
    10921 ttgcaacgag ataaaacagt ccaccatgcg ggcgacttcc gtttccgact gcgcgacgcc
    10981 aaccgtttcg acaatcacta cgtcataacc cgccgcttcg cataacagca ttaattcccg
    11041 tgctcgctga ctggcaccgc ccagatgacc ggaggagggt accgggcgaa taaacgccgc
    11101 ctcggcacgc gccaggtcat tcatgcgggt tttatccccg agaatgctac cgccagtgac
    11161 cgggctgctg ggatcgaccg caataacagc aacctttaat ccctcacgaa tcaacaacat
    11221 gccaaaggct tcaagaaagg tacttttccc cgcgccgggt gtgccggtaa cgcccagtcg
    11281 cagggcgtta ccacagaacg gcataatggc gtcaagcagc tgtgtactta gcgcctgatg
    11341 acgtgggtga cggctttcca ccagcatcat ggcttgggcg agcgtggcac gctcaccctg
    11401 acgtaagcgg cgaatacttt ctgccagcgt ggcttcatta atcatgatgc tggcttatca
    11461 gattcagtac gtcgcgcaca ctgtcgagca taggtgtacc tggaccataa atcgccgcca
    11521 cgccgcgctc ttgcaggaag gcgtaatcct gcggcggaat gacgccaccc gcgaccacgc
    11581 agatatcttc gcgtccccat tttttcagcg cttcgaccag ttccgggatc agcgttttat
    11641 gaccggcagc cagtgaggat gcgcccacta cgtgaacgtc gttttctacg gccaggcggg
    11701 cgatctcttc aggtgtagag aacatcgggc ttaaatctac gtcgaaaccg agatcggaat
    11761 aggcgctggc gatcactttc gcgccgcgat cgtgtccatc ctggcccatc ttagcgatca
    11821 gaatgcgcgg gcgacgacca ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat
    11881 cgaactcgga ggccgatttc tcagactgat gatagctttg cgcaatcacg ccggtaacac
    11941 actggcacca gataacggtc gaaagcgact tccagcgcat cggaaatttc acccagggtg
    12001 gcgcgaacgc gagcggcatt aacagcggca gccagcaggt tttcgttatg ctgtgcggcg
    12061 tgagtcaggg cgttcaacgc ggcggttacg gcggcatcat cacgggtggc gcgaatgcgt
    12121 tccagcgaag caatttgctc gttacgcacc atcacgttgt cgatctcaag tacatcggtt
    12181 tcgtcttcgt gatccagttt gtacttgttg acaccaacga tgacacgctt gccctggtcg
    12241 atcagcgact gttcgcgcgc tgaggcctct tcgatcattc gttttggcag acctgcttcg
    12301 atcgctttcg ccatgccacc ggcttcgtcg atctgttgga taatagctct ggcttgtttg
    12361 acgatttgat cggtcagcga ctcaatgtaa taggatccgg ccagtggatc gacggtgtgg
    12421 cagagttctg attcttcctg gatgatgatc tgggtgttgc gggcaatgcg tgctgagaaa
    12481 tcggtaggca aaccaagcgc ttcgtcaaag gcgttggtat gcagtgactg agtaccgccc
    12541 agcgtcgcag ccagtgcttc aatggtggtg cggataacgt tgttatacgg atcctgttca
    12601 gtcaggctcc agcctgaggt ctggcagtgg gtacgcagcg ccagtgattt cggatcctgt
    12661 gcgccaaatc cactgaccgc ttcgctccat aaataacgtg ccgcacgcaa catggcgacg
    12721 ttcataaaca gatccatgcc gataccgaag aataacgaca ggcgaggagc gaagtcatca
    12781 attttcagtc cggcagagat tgctgctttg atgtactcaa tcccatcggc gagcgtaaat
    12841 gctacctgct gcacgcagtt ggcacccgct tcccccatgt ggtaaccgct gatactgatg
    12901 gtattaaatc gcggcatgtt gccggaacac caggcgatga tgtcggcgat aatgcgcatt
    12961 gacggttttg gtgggtaaat ataggtgttg cggcagaggt actctttgag aatatcgttt
    13021 tgaatggtgc cggtcagttt atcaggtgta acaccttgct cttctgcggc gacgatataa
    13081 aacgccagta ctggtagcac tgcgccattc atggtcatcg aaaccgacat tttatccagc
    13141 gggatctggt cgaacaggac tttcatatct tccacggtgt cgatagcgac gcccgctttg
    13201 ccgacgtcac ccgccacgcg tgggttatcg gagtcgtagc cacggtgggt ggcaaggtca
    13261 aacgcaacgg aaagaccttt ttgcccggcg gccaggttac ggcgataaaa agcgttggac
    13321 tcttttgctg ttgaaaaacc agcatactga cggatggtcc acggttgggc ggtatacata
    13381 gtggcacgcg ggccacgaac gtagggcggc aaaccaggaa gggtacctgt cacctccaga
    13441 ttatcgagat cggcttcggt atacagcggc ttgatggcga tcccttccgc ggtttgctga
    13501 accagcgagt cgacagtttt ctcccgacgg ctcaattcct tgttggcaag ctgttgccac
    13561 tcctgctcgt tagacattat ttgctccgta aaacgcaatc aaaatgaggg tatttgcgct
    13621 tacggtgagc tttttacgtg ggtttgtcgc cagcaaaaaa aatgcattca gacgtcgttt
    13681 tttgccgcct gagatttgtc tattttgtga tgtgattcaa ggaatacaaa aagaagtgag
    13741 gacaacgcct gatatgtgct atcaggcatt atttgatgga ttaatcctga cgaaggactt
    13801 tgtgaccata atcgagtaac gcatcagtga ctttacgcat catgcggctt tcaggagcaa
    13861 agcggtgcca gtagagcatc cgtcgttgaa atagcccagg cgttaagtca atcagttcac
    13921 cgctggccag ctctttctcg atttgcaggt gcgggatcat acagcaggtg gtgccctggc
    13981 gagcaagttg tacgaacgct tctgaagaat taacgatatg gcagggtacg ctgccaggag
    14041 gaagatcgaa gttttgctgc aaaaaggcct ggtgcatatc gtcaagatgg tcaaacgcga
    14101 ccactggcgc tttcaataat gccgaacgcg ttacgccgtt aggaaaatat ttttcggcaa
    14161 acggttttga gctgacgaac agatagtcga gcgcaccgag tttatcgaca agacaactcg
    14221 gcagcgcctg atgttgaata ctcaccgcgc cgaccacttc gccgcggcgc agacgttcct
    14281 gagtacgggt ttcatcttct acctgcaagt tgaggcggat aggcgaatca gccaacacag
    14341 gagccagtgc cggaagcaac cacgtcgcca gactgtcggc gttgaccgcc agtgaaagca
    14401 gcagcggagt cgaaccggtt tgttcatcgc ccagccactc ttcttccagc aactccactt
    14461 ggcgcagcag tgccagcagt ttttgccctt gttccgtcgg gcgcggcggt acggtacgca
    14521 ccaacagcgg ctgcccgaac atattttcca gttgcttaat gcgctgtgag acggctgatt
    14581 gtgtaatgca cagcttttgt gccgcgcgct caaatcctcg ttcacgtatc accgcatcca
    14641 gtgcctgtaa tgttctgtag tccgggcgtt tcattgctgt tgtctgatcc tggtaaatgc
    14701 tttttcctgc actatgacac aattttgtgt caggttgcat ataccgaagc cgtcggcggg
    14761 taacatttgt atcaatggtt aaacgcgcag cttaaaagat cagggatttg cactattgca
    14821 tttcgcttag cgacttcgcg ccctggttat gttgaatggc gtggcgttat tgcctctgtt
    14881 cgcaccgtgg agcggcatac ccagaccgtt gtatgcttta tttgcggggc gaaaggggat
    14941 gcccgccatt gcgcgatatt ttatggatga gttaaccacc cggcttgcca acggggtctg
    15001 aatcgctttt tttgtatata atgcgtgtga aatttcatac cacaggcgaa acgatcatga
    15061 cgcaggatga attgaaaaaa gcagtaggat gggcggcact tcagtatgtt cagcccggca
    15121 ccattgttgg tgtaggtaca ggttccaccg ccgcacactt tattgacgcg ctcggcacga
    15181 tgaaaggcca gattgaaggg gccgtttcca gttcagatgc ttccactgaa aaactgaaaa
    15241 gcctcggcat tcacgttttt gatctcaacg aagtcgacag ccttggcatc tacgttgatg
    15301 gcgcagatga aatcaacggc cacatgcaaa tgatcaaagg cggcggcgcg gcgctgaccc
    15361 gtgaaaaaat cattgcttcg gttgcggaaa aatttatctg tattgccgac gcctccaagc
    15421 aggttgatat tctgggtaaa ttcccgctgc cagtagaagt tatcccgatg gcacgtagtg
    15481 cagtggcgcg ccagctggtg aaactgggcg gtcgtccgga ataccgtcag ggcgtggtga
    15541 ccgataatgg caacgtgatc ctcgacgtcc acggcatgga aatccttgac ccgatagcga
    15601 tggaaaacgc cataaatgcg attcctggcg tggtgactgt tggcttgttt gctaaccgtg
    15661 gcgcggacgt tgcgctgatt ggcacacctg acggtgtcaa aaccattgtg aaatgatctg
    15721 acgggggaac ctcccccgtt aaaaaaattc tcttcattaa atttggtgac atgtgtcacg
    15781 cttttagcag gcaattgtcg attgctctaa ataaatcctc taaaccagca tattcatcca
    15841 agaattacct ttgcgtgata tttcctcaac atcgcgacgc aaacgttcat attgccgcaa
    15901 tattattttt tgatatgttg aaaggcggat gcaaatccgc acacaacatt tcaaaagaca
    15961 ggattgggta aatggcaaag gtatcgctgg agaaagacaa gattaagttt ctgctggtag
    16021 aaggcgtgca ccaaaaggcg ctggaaagcc ttcgtgcagc tggttacacc aacatcgaat
    16081 ttcacaaagg cgcgctggat gatgaacaat taaaagaatc catccgcgat gcccacttca
    16141 tcggcctgcg atcccgtacc catctgactg aagacgtgat caacgccgca gaaaaactgg
    16201 tcgctattgg ctgtttctgt atcggaacaa accaggttga tctggatgcg gcggcaaagc
    16261 gcgggatccc ggtatttaac gcaccgttct caaatacgcg ctctgttgcg gagctggtga
    16321 ttggcgaact gctgctgcta ttgcgcggcg tgccggaagc caatgctaaa gcgcaccgtg
    16381 gcgtgtggaa caaactggcg gcgggttctt ttgaagcgcg cggcaaaaag ctgggtatca
    16441 tcggctacgg tcatattggt acgcaattgg gcattctggc tgaatcgctg ggaatgtatg
    16501 tttactttta tgatattgaa aacaaactgc cgctgggcaa cgccactcag gtacagcatc
    16561 tttctgacct gctgaatatg agcgatgtgg tgagtctgca tgtaccagag aatccgtcca
    16621 ccaaaaatat gatgggcgcg aaagagattt cgctaatgaa gcccggctcg ctgctgatta
    16681 atgcctcgcg cggtacagtg gtggatattc cggcgctgtg tgatgcgctg gcgagcaaac
    16741 atctggcggg ggcggcaatc gacgtattcc cgacggaacc ggcgaccaat agcgatccat
    16801 ttacctctcc actgtgtgaa ttcgacaacg tccttctgac gccacacatt ggcggttcga
    16861 ctcaggaagc gcaggagaat atcggcctgg aagttgcggg taaattgatc aagtattctg
    16921 acaatggctc aacgctctct gcggtgaact tcccggaagt ctcgctgcca ctgcatggtg
    16981 gtcgtcgtct gatgcacatc cacgaaaacc gtccgggcgt gctaactgcg ctgaacaaaa
    17041 tcttcgccga gcagggcgtc aacatcgccg cgcaatatct acaaacttcc gcccagatgg
    17101 gttatgtagt tattgatatt gaagctgacg aagacgttgc cgaaaaagcg ctgcaggcaa
    17161 tgaaagctat tccgggtacc attcgcgccc gtctgctgta ctaattcccc ttctctgaaa
    17221 attaacgggc aggtcactga cttgcccgtt tttttatccc ttctccacac cgttgccgcg
    17281 ttgcgcattc tactttgcga gtctgctcca gaaaataaat caaagtcaga tcaatgcgtt
    17341 gtgtttattt aatggggatt tacgatggcg ggacagcatg gggctgtaac ggtaaagccc
    17401 tcaccgaagc gagggcttaa aggagaaggg ttatgatgcg acttgtcatc atactgattg
    17461 tactgttact gataagtttc ggagcttatt aacagtcagt ctcaggggag gagtcatcct
    17521 cccttaccct tactcactaa attaggtcaa agaatcaacg atgtcaatca gggcgatgcg
    17581 gggtgcatcg ccctgaaccg aactttttga tagccacttc atcgcccacg tttactgccg
    17641 taatagatcc tttttcccaa aaattatctc tgttttgcat ggggtttttc gagcacgctc
    17701 gaagaaaaga tttaaaggta gatcaatgcg ttgtggttat ttaaagagag cttacgatag
    17761 ctgggcagca tggggctgta acggtaaagc cctcaccgaa gcgagggctt gaaggagaag
    17821 ggttatgatg cgacttgcca tcatactgat tgtactgtta ctcataagtt tcccagctta
    17881 ttaacagtca gtcacagggg aggagcaatc ctcccttacc cttactcact aaattaggtt
    17941 aaagaatcaa cgatgtcaat cagggcgatg caggtgtatc gcccttacca ctcccagact
    18001 ttcgacggtg taaccaccgc aggaagaggg atatcccact cttcaacggg gagtttttcc
    18061 accaactgac aatcatgcgc ataacccacc ggttgcgttt tatagtgctg ccagttttgt
    18121 aaggtccgat cataaaaacc accgcccatt cccaggcgct gaccgtactc atcaaaggcg
    18181 accagcggtg tgatcagcac gtctaatcgg gaaaggggta gcacgtcacg cacatccagt
    18241 tttggctcat ggatcttcaa cctgttcatc accagttcgc tttgcggatg gtaattcagg
    18301 aacagcaaat taccggcact aaagggatgc aaaactggaa gatatacgcg cttaccggcg
    18361 cgccagagtt gttctattag cggctgggtg tcgagttcac catcaaaaga gaggaataca
    18421 gcgaccgtat gcgccatcac caccggggga taagtcatca tccgggtagc ggcttgttga
    18481 cccatttcca gctgttgttc cggcgttaac gcacgacgac gttgccgaat cattttgcgg
    18541 atttcttgtc gggataatgt cagtgggagc tctggtagtt gcgtcatggc tggtgccaga
    18601 taagaaggga atctccgaga tgccgccgca ggctgtaacc cttgaaccct tggttcaagg
    18661 tgaatgtgtc gtcgcagttt taaggcttct cggacgaacc gagcatgctc accaaccgcg
    18721 gagcgccaca ttcttgtggt atgaaatatc ggctcagggg actggcccgc ttgcgaacat
    18781 ctcagagaaa ttttgtcttc acggttactc taccacagta aaccgaaaag tgtcattcaa
    18841 agttttggtt agttttttcg gtgatgcgac cttgttcaag taacgcttgt tctatggtct
    18901 gctgcagcat ccgaatacgc tgttccatac ttgccgcgta gtcacgagtc tttgcttttt
    18961 cttgcgctaa ctcatagctg atattcaatg cggcaatgaa gaccaactgt tcagtatttg
    19021 tgactctagt gcgttctttc agatcttgca accgttggtt cagatcgtcc gctgcctgat
    19081 tcaacgcatc cctttggtca ggcgggcagt tcacacgcag tgaacggcca aaaatttgga
    19141 tatcgacggg ttgtgcagac atgccacctt cctgctgatt gactgcgctg ccttcgtctt
    19201 tagacccatg gtctgcgaag gggcgacact atagctaccc tgatgagaag agacaagccc
    19261 ttttctggtc caccagggcc caaagtggta gcatatcatg aatattcctc cctttgacga
    19321 cgaatgctta tgtctataca gaacgaaatg cctggttaca acgaaatgaa ccagtatctg
    19381 aaccaacaag ggacgggtct gacccctgcc gagatgcatg gtttaatcag tgggatgata
    19441 tgtggcggta acgatgacag ctcatggctg ccgctacttc acgacctgac gaacgaaggc
    19501 atggctttcg gtcatgagct ggcacaggca ctgcgtaaaa tgcactctgc caccagcgat
    19561 gccctgcagg atgacggctt cctttttcag ctttatctgc ctgatggcga tgatgtcagc
    19621 gttttcgatc gggctgatgc actggcaggt tgggtcaatc acttcctgct tggtcttggc
    19681 gttacgcaac cgaagctgga caaagtgacc ggcgaaaccg gtgaagccat cgacgatctg
    19741 cgtaacatcg cgcagctggg ctacgacgaa gacgaagatc aggaagagct tgaaatgtcg
    19801 cttgaagaga tcatcgaata cgttcgtgtt gccgcgctgt tatgccacga cacctttact
    19861 catccgcaac cgaccgcgcc agaagtacaa aaaccgactc tacactaaaa acaaaaaacg
    19921 taaggagagt gttatgagtg agatatcccg gcaagagttt cagcgtcgcc gtcaggccct
    19981 ggtggagcaa atgcaacccg gcagcgccgc gctgattttt gctgcaccag aagtaacacg
    20041 tagcgccgac agcgaatacc cctatcgtca gaacagtgac ttctggtact tcaccggctt
    20101 taacgaaccg gaagcggtgc tggtgctgat taaaagcgat gacactcata accacagcgt
    20161 tctgtttaac cgcgttcgcg acctgacggc ggagatctgg tttggccgtc gcttaggcca
    20221 ggatgccgcg ccagagaaac tgggcgttga ccgcgcactg gcattcagcg aaatcaatca
    20281 gcaactttat caactactta acggcctgga tgtggtttac catgcccagg acgaatatgc
    20341 atatgctgat gaaatcgtga acagtgcgct ggaaaaactg cgtaaaggtt cgcggcaaaa
    20401 tctcaccgca ccggcaacga tgatcgactg gcgtcctgtt gttcatgaaa tgcgcctgtt
    20461 caaatcgcca gaagagattg ccgtactccg ccgcgcggga gaaatcaccg ccatggcaca
    20521 tacacgggcg atggaaaaat gccgtccggg aatgttcgag taccatctgg aaggcgaaat
    20581 tcaccacgaa tttaaccgcc acggtgcgcg ctatccttcc tataacacca ttgtaggcag
    20641 cggtgaaaac ggctgcattc tgcactacac cgaaaacgag agtgaactgc gcgacggtga
    20701 cctggtgttg attgacgcgg gttgtgaata caaaggttac gcgggcgata ttacccgcac
    20761 cttcccggtc aacggcaaat tcacccaggc ccagcgtgaa atctacgaca ttgtgctgga
    20821 gtctctcgaa accagcctgc gcctgtatcg tccgggaact tccattctgg aagtcactgg
    20881 tgaagtggtg cgcatcatgg ttagcggcct ggtaaaactc ggcatcctga aaggtaatgt
    20941 tgatgaactg atcgctcaga acgcccatcg tcctttcttt atgcatggcc ttagccactg
    21001 gttaggactg gatgtccatg acgttggcgt ttatggtcag gatcgctcgc gcattctgga
    21061 accggggatg gtactgaccg tagagcctgg tctgtatatc gcgccggatg cggatgtgcc
    21121 agaacaatat cgcggtatcg gcattcgtat tgaagacgac attgtgatta ccgaaaccgg
    21181 caacgaaaac ctcaccgcca gcgtggtgaa aaagccggaa gaaatcgaag cgttgatggc
    21241 tgctgcgaga aagcaatgag cgtaatcatc gtcggtggcg gcatggcggg cgcgacgctg
    21301 gcgctggcta tttcccggtt aagtcacggg gcgctgccgg tacatttgat tgaagcgact
    21361 gcgccagagt cacatgctca tccgggcttt gatggacgag cgatagcgct ggcggcgggt
    21421 acctgtcagc aactggcgcg catcggcgtc tggcaatctc tggctgattg cgcaaccgcc
    21481 atcactaccg tgcatgtcag cgatcgtggt catgctggat tcgtcaccct cgtcgcagaa
    21541 gattaccaac tggcggcgct ggggcaggtt gtcgaattgc ataatgtcgg gcaacggctg
    21601 tttgcattgc tgcgaaaagc acctggcgta atgctgcatt gccccgatcg cgtagctaac
    21661 gttgcccgta ctcagagtca cgttgaagtg acgctggaga gcggcgagat gctgacgggc
    21721 cgcgtgctga tagccgctga tggcacccat tcagcgttag ccaccgcgtg cggcgttgat
    21781 tggcagcagg agccttacga acaactggca gtgattgcca acgttgctac ttctgttgcg
    21841 cacgaaggcc gcgcttttga acgcttcacg caacatggcc cgctggcgat gttgccgatg
    21901 tctgacggac gctgttcgct ggtctggtgt catccactgg aacggcgcga agaggtgctg
    21961 tcgtggagcg acgagaagtt ttgccgtgaa ctccagtctg cctttggctg gcgactgggg
    22021 caaattaccc acgccggtaa acgcagtgct tatccgctgg cattaacccg cgccgccaga
    22081 ccgattaccc atcgcactgt gctggtgggc aatgcggcgc aaactctgca cccgattgcc
    22141 gggcaagggt ttaacctcgg tatgcgagat gtcatgagcc ttgcggaaac cctgactcag
    22201 gcgcatgagc gcggagaaga catcggtgat tacggcatat tgtgccgtta tcagcagcgt
    22261 cgacagagcg atcgcgaagc aaccatcggc gtcacggaca gcctggtaca tctttttgcc
    22321 aaccgttggg caccgctggt tgtcgggcgc aacatcggac tgatgacgat ggaattattc
    22381 accccggcac gcgatgtgct ggcgcagcgc accctcggtt gggtggcgcg ttgaaggcgg
    22441 ttttaaggaa taaacaatgc aaagtgttga tgtagccatt gttggcggcg gcatggtggg
    22501 gctggcggtt gcctgcggct tacaggggag cggcttacgc gttgccgtac tggagcagcg
    22561 cgtaccggaa cctctggcgg tagatgcact accacaactg cgcgtttcgg ctatcaatgc
    22621 cgccagcgaa aaattactca cccgtcttgg cgtctggcag gacattctcg ctcgtagggc
    22681 tagttgctat cacggcatgg aagtgtggga caaagacagt tttggtcaca tctcgtttga
    22741 cgatcaaagc atgggctata gccatcttgg gcacatcgtt gaaaactcag tgattcacta
    22801 cgcgctgtgg aacaaagcgc agcagtcgtc agatattact ctgttggccc ccgcagaatt
    22861 acagcaggtc gcctggggag aaaatgaaac cttcctgacg ctgaaagatg gcagcatgtt
    22921 aacggcgcgt ctggtgattg gcgcggacgg tgctaattcc tggttgcgca acaaagcaga
    22981 tattccgctg actttctggg attatcagca tcacgcgctg gtagcgacca ttcgcacgga
    23041 agaaccgcat gatgcggtgg cgcggcaggt tttccatggc gaaggcattc tggccttttt
    23101 accgcttagc gatccgcatc tttgctcgat tgtctggtca ctgtcgccag aggaagcgca
    23161 gcggatgcag caggcaagtg aagacgaatt taatcgcgcg ttaaatatcg cttttgataa
    23221 tcgccttggc ttatgcaagg ttgagagcgc gcgtcaggtg ttcccgctga cggggcgtta
    23281 tgcgcgccag tttgccgcac accgtctggc gctggtgggc gacgccgcgc ataccattca
    23341 cccactggcg gggcagggcg ttaatctcgg atttatggac gccgcagagc tgattgctga
    23401 actgaaacgg ttgcatcgtc agggtaaaga catcgggcag tacatttacc tgcgccgcta
    23461 tgaacgtagc cgcaagcaca gcgcggcgct gatgctggct ggtatgcagg gttttcgcga
    23521 tctcttttcc ggcgctaatc cggcgaaaaa actgctgcgt gatattggtt tgaaactggc
    23581 cgacacgctt cctggcgtta agccgcaact tatccgccag gcaatgggat taaacgattt
    23641 gcctgaatgg ctgcgttaaa aatttctcct ctgttgttta tttgataccc atcacacttt
    23701 catctcccgg ttttttcgcc gggagatttt cctcatttga aataaactaa tttcacctcc
    23761 gttttcgcat tatattttct aatgccatga ttttttgatt tagtgttttt tgacattttt
    23821 ttagctctta atattgtctt atccaaattg actttctcat cacatcatct ttgtatagaa
    23881 attggtgtat tttttggttt tttattctgt cgcgattttt gcatttttta accataagct
    23941 aatgtgatga tcaattttac cttatggtta acagtctgtt tcggtggtaa gttcaggcaa
    24001 aagagaacga ttgcgttggg gaccgggagt ggctccgatg ctgggttttg tggtgataat
    24061 ttcaccatga aaaagttgtc agccccgctt attcaatgag gacaagatgg cacaacagac
    24121 tcctttgtac gaacaacaca cgctttgcgg cgctcgcatg gtggatttcc acggctggat
    24181 gatgccgctg cattacggtt cgcaaatcga cgaacatcat gcggtacgta ccgatgccgg
    24241 aatgtttgat gtgtcgcata tgaccatcgt cgatctccgc ggcagccgca cccgggagtt
    24301 tctgcgttat ctgctggcga acgatgtggc gaagctcacc aaaagcggca aagcccttta
    24361 ctcggggatg ttgaatgcct ctggcggtgt gatagatgat ctcatcgtct actactttac
    24421 tgaagatttc tttcgcctcg ttgttaactc cgccacccgc gaaaaagacc tctcctggat
    24481 tacccaacac gctgaacctt tcggcatcga aattaccgtt cgtgatgacc tttccatgat
    24541 tgccgtgcaa gggccgaatg ctcaggcaaa agctgccaca ctgtttaatg atgctcagcg
    24601 tcaggcggtg gaagggatga aaccgttctt tggcgtgcag gtgggcgatc tgtttattgc
    24661 caccactggt tataccggtg aagcgggcta tgaaattgcg ctgcccaatg aaaaagcggc
    24721 cgatttctgg cgtgcgctgg tggaagcggg cgttaagccc tgcggtctgg gggcgcgtga
    24781 cacgctgcgt ctggaagcgg gcatgaatct ttatggtcag gagatggacg aaactatttc
    24841 tcctttagcc gccaacatgg gctggactat cgcctgggaa ccgacagatc gtgactttat
    24901 cggtcgtgaa gccctggaag tgcagcgtga gcatggtaca gaaaaactgg ttggtctggt
    24961 gatgaccgaa aaaggcgtgc tgcgtaatga actgccggta cgctttaccg atgcgcaggg
    25021 caaccagcat gaaggcatta tcaccagcgg tactttctcc ccgacgctgg gttacagcat
    25081 tgcgctggcg cgcgtgccgg aaggtattgg cgaaacggcg attgtgcaaa ttcgcaaccg
    25141 tgaaatgccg gttaaagtga caaaacctgt ttttgtgcgt aacggcaaag ccgtcgcgtg
    25201 atttactttt ttggagattg attgatgagc aacgtaccag cagaactgaa atacagcaaa
    25261 gaacacgaat ggctgcgtaa agaagccgac ggcacttaca ccgttggtat taccgaacat
    25321 gctcaggagc tgttaggcga tatggtgttt gttgacctgc cggaagtggg cgcaacggtt
    25381 agcgcgggcg atgactgcgc ggttgccgaa tcggtaaaag cggcgtcaga catttatgcg
    25441 ccagtaagcg gtgaaatcgt ggcggtaaac gacgcactga gcgattcccc ggaactggtg
    25501 aacagcgaac cgtatgcagg cggctggatc tttaaaatca aagccagcga tgaaagcgaa
    25561 ctggaatcac tgctggatgc gaccgcatac gaagcattgt tagaagatga gtaacggctt
    25621 tattcctctt ctgcgggaga ggatcagggt gaggaaaatt tatgcctcac cctcactctc
    25681 ttcgtaagga gagaggttca caattcactg cacgtttcag gaaccatcgc tcatgacaca
    25741 gacgttaagc cagcttgaaa acagcggcgc ttttattgaa cgccatatcg gaccggacgc
    25801 cgcgcaacag caagaaatgc tgaatgccgt tggtgcacaa tcgttaaacg cgctgaccgg
    25861 ccagattgtg ccgaaagata ttcagcttgc gacaccaccg caggttggcg caccggcgac
    25921 cgaatacgcc gcactggcag aactcaaggc tattgccagt cgcaataaac gcttcacgtc
    25981 ttacatcggc atgggttaca ccgccgtgca gctaccgccg gttatcctgc gtaacatgct
    26041 ggaaaatccg ggctggtata ccgcgtacac tccgtatcaa cctgaagtct cccagggccg
    26101 ccttgaagca ctgctcaact tccagcaggt aacgctggat ttgactggac tggatatggc
    26161 ctctgcttct cttctggacg aggccaccgc tgccgccgaa gcaatggcga tggcgaaacg
    26221 cgtcagcaaa ctgaaaaatg ccaaccgctt cttcgtggct tccaatgtgc atccgcaaac
    26281 gctggatgtg gtccgtactc gtgccgaaac ctttggtttt gaagtgattg tcgatgacgc
    26341 gcaaaaagtg ctcgaccatc aggacgtctt cggcgtgctg ttacagcagg taggcactac
    26401 cggtgaaatt cacgactaca ctgcgcttat tagcgaactg aaatcacgca aaattgtggt
    26461 cagcgttgcc gccgatatta tggcgctggt gctgttaact gcgccgggta aacagggcgc
    26521 ggatattgtt tttggttcgg cgcaacgctt cggcgtgccg atgggctacg gtggcccaca
    26581 cgcggcattc tttgcggcga aagatgaata caaacgctca atgccgggcc gtattatcgg
    26641 tgtatcgaaa gatgcagctg gcaataccgc gctgcgcatg gcgatgcaga ctcgcgagca
    26701 acatattcgt cgtgagaaag cgaactccaa catttgtact tcccaggtac tgctggcaaa
    26761 catcgccagc ctgtatgccg tttatcacgg cccggttggc ctgaaacgta tcgctaaccg
    26821 cattcaccgt ctgaccgata ttctggcggc gggcctgcaa caaaaaggtc tgaaactgcg
    26881 ccatgcgcac tatttcgaca ccttgtgtgt ggaagtggcc gacaaagcgg gcgtactgac
    26941 gcgtgccgaa gcggctgaaa tcaacctgcg tagcgatatc ctgaacgccg tggggatcac
    27001 ccttgatgaa accaccacgc gtgaaaacgt aatgcagctt ttcagtgtgc tgctgggcga
    27061 taaccacggg ctggagatcg acacgctgga caaagacgtg gctcacgaca gccgctctat
    27121 ccagcctgcg atgctgcgcg acgacgaaat cctcacccat ccggtgttta atcgctatca
    27181 cagcgaaacc gaaatgatgc gctatatgca ctcgctggag cgtaaagatc tggcgctgaa
    27241 tcaggcgatg atcccgctgg gttcctgcac catgaaactg aacgccgccg ccgagatgat
    27301 cccaatcacc tggccggaat ttgccgaact gcacccgttt tgcccgccgg aacaggctga
    27361 aggttatcag cagatgattg cgcagctggc tgactggctg gtgaaactga ccggttacga
    27421 cgccgtttgt atgcagccga actccggcgc acagggcgaa tacgcgggcc tgctggcgat
    27481 tcgtcattat catgaaagcc gcaacgaagg gcatcgcgat atctgcctga tcccggcttc
    27541 tgcgcacgga actaaccccg cttctgcaca tatggcagga atgcaggtgg tggttgtggc
    27601 gtgtgataaa aacggcaaca tcgatctgac tgatctgcgc gcgaaagcgg aacaggcggg
    27661 cgataacctc tcctgtatca tggtgactta tccttctacc cacggcgtgt atgaagaaac
    27721 gatccgtgaa gtgtgtgaag tcgtgcatca gttcggcggt caggtttacc ttgatggcgc
    27781 gaacatgaac gcccaggttg gcatcacctc gccgggcttt attggcgcgg acgtttcgca
    27841 cctcaacctg cataaaactt tctgcattcc gcacggcggt ggtggtccgg gtatgggacc
    27901 gatcggtgtg aaagcgcatc tggcaccgtt tgtaccgggt catagcgtgg tgcaaatcga
    27961 aggcatgtta acccgtcagg gcgcagtttc tgcggcaccg ttcggtagcg cctctatcct
    28021 gccaatcagc tggatgtaca tccgcatgat gggcgcagaa gggctgaaaa aagcaagcca
    28081 ggtggcaatc ctcaacgcca actatattgc cagccgcctg caggatgcct tcccggtgct
    28141 gtataccggt cgcgacggtc gcgtggcgca cgaatgtatt ctcgatattc gcccgctgaa
    28201 agaagaaacc ggcatcagcg agctggatat tgccaagcgc ctgatcgact acggtttcca
    28261 cgcgccgacg atgtcgttcc cggtggcggg tacgctgatg gttgaaccga ctgaatctga
    28321 aagcaaagtg gaactggatc gctttatcga cgcgatgctg gctatccgcg cagaaattga
    28381 ccaggtgaaa gccggtgtct ggccgctgga agataacccg ctggtgaacg cgccgcacat
    28441 tcagagcgaa ctggtcgccg agtgggcgca tccgtacagc cgtgaagttg cggtattccc
    28501 ggcaggcgtg gcagacaaat actggccgac ggtgaaacgt ctggatgatg tttacggcga
    28561 ccgtaacctg ttctgctcct gcgtaccgat tagcgaatac cagtaattca ctgattcgag
    28621 tattttctaa aggcgcttcg gcgccttttt agtcagatga caaagtacaa aagtgctcag
    28681 acagtcccct cgcccctttg gggagagggt tagggtgagg ggaacaggcc ggcactggcg
    28741 cgaatattta ccctcacccc gaccctctcc ctgaaagggc gagggggaaa agcgtgccaa
    28801 cattgaagat tgagccagtt tgttagcaat ctcaaagata cgtcaacgaa ttaatttttc
    28861 tcggaaaaac aaatggctat agcacttgtg accggtggca gttgcggcat cgggcgggca
    28921 actgcattac tgttggcgca agaagggtat acggtggcgg ttaattatca gcaaaacctc
    28981 cacgcggcgc aggaagtgat gaacttaata atgcaagccg gtggcaaagc attcgtgctc
    29041 caggcggata tcagcgacga aaaccaggtc gttgcgatgt ttacagcaat cgatcagcac
    29101 gatgaaccgc tagcagcgct ggtcaataac gccgggatct tgtttaccca gtgcaccgtt
    29161 gaaaacctta ccgcagagcg aatcaaccga gtactttcca ccaacgtgac gggatatttt
    29221 ctctgctgcc gcgaggcggt aaaacgcatg gcgcttaaaa atggtggcag tggcggcgct
    29281 atcgtcaatg tctcttcggt ggcctcacga ttgggttcgc caggggaata tgttgattac
    29341 gcggcatcga aaggggcgat tgatacgtta accaccggac tatcgctgga agtcgccgcg
    29401 caggggatcc gcgttaactg cgtgcggcca gggtttattt ataccgaaat gcacgccagc
    29461 ggcggcgagc ctggacgcgt cgatcgcgtt aagtcgaaca tccccatgca gcgtggtgga
    29521 caggcagaag aggtcgcgca ggccattgtc tggctactaa gtgataaagc ctcttacgtc
    29581 acgggaagtt ttatcgattt ggcgggcggg aaataaaata gggaagttgt ctgaccggat
    29641 gcaacaagta ttgcatccgg tacttcatcg acttaaagct tctcgccgtt gctggcaatc
    29701 acctctttgt accagttaaa gctcttctta cgtgaacgcg acatatcgcc agtaccgtcg
    29761 tcatgtttat tcacatagat aaagccgtag cgtttgctgt actgcccggt agtgaatgac
    29821 acgcagtcga tgcagcccca cggtgtgtag cccatcagat ccacaccatc gtaagtcact
    29881 gcttttttca tctcttcaat atgggcgcgc aggtagtcaa tgcggtagtc gtcgttgatg
    29941 ctgccatctt cttccacttt gtcgtaagcg ccaaaaccgt tttcgacaat aaacagcggc
    30001 ctctgataac gctcatacag ttcgcaaagg gcatagcgca gacctactgg atcaatctgc
    30061 cagccccagt cagacgcttt aacatacggg ttcggtacgc tgccttcaaa accagagatc
    30121 gcatcgccgg tgccgccttc ggccttcact gcgttggtca tgtaatagct gaaaccaaga
    30181 taatcgcagg tgccttcgcg cagtacatcg agatcgccgt cttccatttt gatattaaat
    30241 ccgcgacgtt cccactcgtt caatacatag gacgggtaat agccgcatag ctgcacatcg
    30301 gtaaagacgt agcgttcgcg catcgactcc tgagcgaaca tcacatcgtc cgggttacat
    30361 gagtagggat agagcggcac catcgccagc atacagccga ctttcatctc cgggttaatg
    30421 cgacgcgcgg ctttcactgc cagggcgctg gcgacaaact ggtgatgcag cacctggtac
    30481 atcgtctctt ccgggttgtc atgctcggta tacaccacgc cggagcagca gtaaccaaac
    30541 agcggcgcac gccagttacg ctggttgttt atttcgttga aggtcatcca gtatttgact
    30601 ttatgcttgt agcgttcgaa taccacttcc gcgaaacgga caaagaaatc aacgacttta
    30661 cggttggtcc agctaccgta ttgctgcacc agatgcagcg gcatttcaaa gtgggagagg
    30721 gttatcaccg gttcgatgtt gtatttcagc agttcatcga acagagagtc gtaaaacttc
    30781 agcccttctt cgtttggctg agcttcatca ccttttggaa aaatacgcgt ccaagcgatg
    30841 gaagtccgaa aacatttgaa gcacatttcg gcaaatagct tgatgtcttc cttgtagtga
    30901 ccataaaaat caacggcttc atggtttgga tagtattttc ctggcaagac ttctttggtg
    30961 atttcgcgcg gcacgccgtg tgcgccaccg gtcagaacgt cacaaatgct cggtcctttg
    31021 ccgcctttgt tccagccgcc ttcgacctga tgagcggcaa cggcgccacc ccataagaaa
    31081 tctttcggta aggtgagttt tttcactatc ataggctcca gatcagtcaa ttcttacagg
    31141 aaatagtacc atgcagccaa acgacatcac tttttttcaa cgtttccagg atgacattct
    31201 ggctgggcgt aaaaccatca ccatccgcga cgagtctgaa tcgcacttca aaacgggtga
    31261 tgtgcttcgt gtcggacgtt ttgaagatga cggttatttt tgcacgattg aagtcaccgc
    31321 aacctcaacc gtaacgctgg atacactgac ggaaaaacat gcagagcagg aaaatatgac
    31381 cctgcctgaa ctgaaaaagg tcattgctga catctatccc ggtcagacac aattttatat
    31441 gattgaattt aaatgtcttt aaattgaact cacgcctgtt agttgaaatt tcgcatttaa
    31501 tttcatgatt tttaggagat tagtctcaaa tcagaattta ttttagctaa caggtgttca
    31561 ctggaactat tctcagttac gctggagagg tatacatgtg cgtgtacgtt tctctggagt
    31621 aagttatggt tcagaagccc ctcattaagc agggatattc gctggcagag gaaattgcca
    31681 acagcgtcag tcacggcatt gggctggtgt ttggtatcgt tgggctggtg ttgctactgg
    31741 ttcaggcggt ggatctcaat gccagcgcca cagcgataac cagttacagc ctctatggcg
    31801 gcagtatgat cctgctgttc ctcgcttcga cgctctatca cgccattcct catcaacggg
    31861 caaaaatgtg gctgaagaaa tttgaccatt gcgctattta cctgttgatt gccggaacct
    31921 acacgccgtt tttgctggtg gggctggatt ctccgttagc gcgcgggttg atgattgtta
    31981 tctggagcct ggcattgctg gggattctgt tcaaactgac catcgcgcac cgattcaaaa
    32041 tcttatctct ggtgacttat ctggcgatgg gctggctgtc gctggtggta atttatgaaa
    32101 tggcagttaa gctcgcggcg ggcagcgtta ccttactggc ggtaggcggc gtggtttatt
    32161 cgctcggggt gattttctac gtctgcaaac gcattccata caaccatgcc atctggcacg
    32221 gcttcgtgct cggcggtagt gtgtgccact ttctggcgat ctatttgtat attgggcagg
    32281 cgtaatgggt atgcctttgt aggcctgata agacgcgtca gcgtcgcatc aggcatcgga
    32341 gcgcaaccgc cggatgcggc gtgaacgcct tatccgatct acgggcctcg tccctgttgt
    32401 gataaaggca tcaaatccca gacatgataa gacgcgtcag cgtcgcatca ggcgataaat
    32461 gcctgatgcg atacagagat ttactcttcg agcgaatacg gcagcggctc gatatgcagt
    32521 gtattcgcat cgtcgcgtac gcggaagatg ctgtccggtt ccatatcgtt attcatgacg
    32581 acctgtacca cgacctgacc atcttccagt tttaccgcag ccagcgccgt accggtacga
    32641 cgccagttct cgcccatttt cagctctaag tcttcaccag cttccggcag tcggctggca
    32701 ctacctgcca gcaaccagag cgcgcgtttg ttggcaccac ggaattttgc tcgcgccacc
    32761 atctcttgtc cggtataaca gcctttctta aagctgatac cgcccagcgc ctggagattg
    32821 gtcgcctgtg ggataaactg cccgctgttg gcggcatcaa tcaccgggaa accagcttca
    32881 atgtttaatg ccagccactg ttggctattg ttcagttccg cttcaccgcg cagtttatcg
    32941 gtcagcatat tagcagtagc ttcatcggtt acgatcagga aacgttctgc cgggtgttca
    33001 aaccatagca aagtggtcgc gccttctttg actacctgtt tttctttcga aggcagttcg
    33061 ctaaagagat ttgccagcgc ggcgcgcgcc tgaaaaccgg caacaccaag cagcacacgc
    33121 tcgtcgtctg gcgcgatggt cactttagag aataccgcat attttttcag ttcagtcagc
    33181 tgcggttcac gcacgctgcg ccgttcaatc catgcaaagc catcgccgtc gcggaacaga
    33241 cgtaaattgc tccacatttt acctttggcg tcgcaatggg cggcgagcag gtgctgatct
    33301 tctgccatct ggctgacatc tgctgtcacc tgaccctgca tatatttttc gctgtccgcg
    33361 ccagtaatgg tggcaagcgc ccagtcatca agcgtcatca gcgtcagcgg taaacgggca
    33421 gaagccgtag gctgacgtgg aggaaaaggt gtaaaagcca taatagagtc ctgagttgct
    33481 taacggcgta ttaatgacta atggtaaaag agctatgagg caatgcaagt gctttcacta
    33541 cgcgatctgt gccttctgcg aggcgactgc caaaaaaatg aagcccttga attgttgtga
    33601 attaatttcg cgatcgcgat ggtgtttact gatattcccg taaaagaagt cgaaaaacac
    33661 gttacaatag ccgggtactg gattttcgtg agacaggaag aagatggaca ttaacaacaa
    33721 agcccgcatt cattgggcat gccgccgtgg tatgcgcgaa ctcgatattt caatcatgcc
    33781 gtttttcgaa catgagtacg acagcttaag cgatgacgaa aaacgcatct ttattcgtct
    33841 gctggaatgt gacgatccgg acctgtttaa ctggctgatg aatcacggta aaccggccga
    33901 tgcagaactg gaaataatgg tccgactcat ccagacacgg aaccgggaac gtggtcctgt
    33961 ggcaatctga tttgcgcgtc tcctggcgcg cacagtggct ttccttgctg attcatgggc
    34021 tggttgccgc tgttatttta ctcatgccct ggccgctcag ttacaccccg ttatggatgg
    34081 tgttactttc gctggtggta tttgattgcg ttcgcagcca gcggcgtatt aatgctcgcc
    34141 agggggaaat tcgcttgttg atggacgggc gtttgcgttg gcaagggcag gagtggagca
    34201 tcgtcaaagc accgtggatg attaagagcg gcatgatgct gcgtttacgt tctgatggcg
    34261 gtaaacggca acatttatgg ctggcagccg acagcatgga cgaagccgaa tggcgggatt
    34321 tacggcggat tttgttgcaa caagagacgc aaagataaag atgacggcaa agcatatgcg
    34381 ctaagccgtc agtgagatca ggcgtaatgc tctgccattt cgttgaggat ttgctcgcac
    34441 cagctctgaa tacgctcgtc gctaaggtca tactggttag tttcatccag cgccagaccc
    34501 acgaacagtt gcccgtcagc aatcaccggt ttcgggctgg taaattcata tccttccgtt
    34561 ggccagtagc cgacgaactt cacgcctttg gtcgagagtt tgtcatgcag cataccgagc
    34621 gcatcgagga accactcgcc gtatcccagt tgatcgccaa gcccatacag cgcaacaatt
    34681 ttaccttcaa ggttcaggtc gtcgagctga tcccagacgg cttcccagtc ttcctggatt
    34741 tcaccaaaat cccaggtcgg gatacccaga atgagcacat cgtactgctc cattaatttc
    34801 ggggagtcgt ccttgaggtt atgtaaggtc accagttctg ggccgataat atcgcggatt
    34861 ttttctgccg ccatttcggt gtaacaggtg ctggaaccgt aaaaaagacc catattcata
    34921 acgtaagctt ctcgattctg ctgtggcttt tgcagctagt gtatcagaat cgctttagtc
    34981 acggcacaat aaggcataat ggatcaaatg agtgaccata aggggcgcaa gtgaaacagg
    35041 atctggcacg catcgagcag tttcttgatg ctctgtggct ggaaaaaaat ctggctgaaa
    35101 atacgttgaa cgcttaccgt cgcgatctgt caatgatggt ggagtggttg catcaccgcg
    35161 ggttgacgct ggcgacggcg caaagtgacg atttgcaggc attactggca gaacggctgg
    35221 agggcgggta taaagccacc agctcagcgc gtttgctgag tgcagtgcgc cgattgttcc
    35281 agtatcttta tcgcgaaaag tttcgtgaag acgatcccag tgcgcatctc gcttcaccga
    35341 aattgcccca gcgtttgcca aaagatttaa gcgaagcgca ggtcgaacgt ttattacagg
    35401 cgccattaat tgatcagcca ctggagctac gcgataaagc catgcttgaa gtgttgtatg
    35461 ctaccgggct gcgtgtctct gaactggtcg ggctgacaat gagtgatatc agcctgcgtc
    35521 agggcgtagt gcgggtcatt ggtaaaggca acaaagagcg cctggtgccg ttaggggaag
    35581 aggcggttta ctggctggaa acctatctgg aacatgggcg tccgtggctg ctgaatggtg
    35641 tgtcaattga cgtgttgttt cccagccagc gtgcgcagca gatgacgcga cagaccttct
    35701 ggcaccgcat taaacattat gccgtgctgg cgggtatcga cagcgaaaag ctgtcaccgc
    35761 atgtgttgcg tcacgctttt gccactcatt tattaaatca tggtgcggat ttacgcgtgg
    35821 tgcagatgct actgggccac agcgatcttt ccaccacgca aatttatacg catgtcgcta
    35881 ccgagcgtct gcggcaactt catcaacagc atcacccgcg ggcgtgatgt ctgaaaagaa
    35941 cgggaagatt tatgaagaaa ggttttatgt tgtttacttt gttagcggcg ttttcaggct
    36001 ttgctcaggc tgatgacgcg gcaattcaac aaacgttagc caaaatgggc atcaaaagca
    36061 gcgatattca gcccgcgccc gtagctggca tgaagacagt tctgactaac agcggtgtgt
    36121 tgtacatcac cgatgatggt aaacatatca ttcaggggcc aatgtatgac gttagtggca
    36181 cggctccggt caatgtcacc aataagatgc tgttaaagca gttgaatgcg cttgaaaaag
    36241 agatgatcgt ttataaagcg ccgcaggaaa aacacgtcat caccgtgttt actgatatta
    36301 cctgtggtta ctgccacaaa ctgcatgagc aaatggcaga ctataacgcg ctggggatca
    36361 ccgtgcgtta tcttgctttc ccgcgccagg ggctggacag cgatgcagag aaagaaatga
    36421 aagctatctg gtgtgcgaaa gataaaaaca aagcgtttga tgatgtgatg gcaggtaaaa
    36481 gcgtcgcacc agccagctgc gacgtggata ttgccgacca ttacgcactt ggcgtccagc
    36541 ttggcgttag cggtactccg gcagttgtgc tgagcaatgg cacacttgtt ccgggttacc
    36601 agccgccgaa agacatgaaa gaattcctcg acgaacacca aaaaatgacc agcggtaaat
    36661 aattcgcgtg aaacaacaga tacaacttcg tcgccgtgaa gtcgatgaaa cggcagactt
    36721 gcccgctgaa ttgcctccct tgctgcgccg tttatacgcc agccggggag tacgcagtgc
    36781 gcaagaactg gaacgcagtg ttaaaggtat gttgccctgg cagcaactaa gcggtgtcga
    36841 aaaggccgtt gagatccttt acaacgcttt tcgcgaagga acgcggatta ttgtggtcgg
    36901 tgatttcgac gccgacggcg cgaccagcac ggctctaagc gtgctggcga tgcgctcgct
    36961 tggttgcagc aatatcgact acctggtacc aaaccgtttc gaagacggtt acggcttaag
    37021 cccggaagtg gtcgatcagg cccatgcccg tggcgcgcag ttaattgtca cggtggataa
    37081 cggtatttcc tcccatgcgg gagttgagca cgctcgctcg ttgggcatcc cggttattgt
    37141 taccgatcac catttgccgg gcgacacatt acccgcagcg gaagcgatca ttaaccctaa
    37201 cttgcgcgac tgtaatttcc cgtcgaaatc actggcaggc gtgggtgtgg cgttttatct
    37261 gatgctggcg ctgcgcacct ttttgcgcga tcagggctgg tttgatgagc gcggcatcgc
    37321 aattcctaac ctggcagaac tgctggatct ggtagcgctg gggacagtag cggacgtcgt
    37381 gccgctggac gctaataatc gcattctgac ctggcagggg atgagtcgca tccgtgccgg
    37441 aaagtgccgt ccagggatta aagcgctgct ggaagtggca aatcgcgatg cacaaaaact
    37501 agccgccagc gatttaggtt ttgcgctggg accgcgtctc aatgctgccg ggcgactgga
    37561 tgatatgtcc gttggtgtgg cgctgttgct gtgcgacaac atcggcgaag cgcgcgtgct
    37621 ggcaaatgaa ctcgatgcgc taaaccagac gcgaaaagag atcgaacaag gaatgcaggt
    37681 tgaagccctg accctgtgcg agaaactgga gcgcagccgt gacacgctac ccggcgggct
    37741 ggcaatgtat caccccgaat ggcatcaggg cgttgtcggt attctggctt cgcgtatcaa
    37801 agagcgtttt caccgtccgg ttatcgcctt tgcgccagca ggtgacggta cgctgaaagg
    37861 ttccggtcgc tccattcagg ggctgcatat gcgtgatgcg ctggagcgat tagacacact
    37921 ctaccccggc atgatgctca agtttggcgg tcatgcgatg gcggcgggtt tgtcgctgga
    37981 agaggataaa ttcgaactct ttcaacaacg gtttggcgaa ctggttactg agtggctggc
    38041 cccttcgcta ttgcaaggcg aagtggtgtc agacggtccg ttaagcccgg ccgaaatgac
    38101 catggaagtg gcgcagctgc tgcgcgatgc tggcccgtgg gggcagatgt tcccggagcc
    38161 gctgtttgac ggtcatttcc gtctgctgca acagcggctg gtgggcgaac gtcatttgaa
    38221 ggtgatggtc gaaccggtcg gcggcggtcc actgctggat ggtattgctt ttaatgtcga
    38281 taccgctctc tggccggata acggcgtgcg cgaagtgcaa ctggcttata agctcgatat
    38341 caacgagttt cgcggcaacc gcagcctgca aattatcatc gacaatatct ggccaattta
    38401 gcgtcatctt ctctataaaa aagagcgtgg attgggtaca atcccgctct tatcaccgca
    38461 ttttgactag ctcaataaaa gaaatcagac catgtttgaa attaatccgg taaataatcg
    38521 cattcaggac ctcacggaac gctccgacgt tcttaggggg tatctttgac tacgacgcca
    38581 agaaagagcg tctggaagaa gtaaacgccg agctggaaca gccggatgtc tggaacgaac
    38641 ccgaacgcgc acaggcgctg ggtaaagagc gttcctccct cgaagccgtt gtcgacaccc
    38701 tcgaccaaat gaaacagggg ctggaagatg tttctggtct gctggaactg gctgtagaag
    38761 ctgacgacga agaaaccttt aacgaagccg ttgctgaact cgacgccctg gaagaaaaac
    38821 tggcgcagct tgagttccgc cgtatgttct ctggcgaata tgacagcgcc gactgttacc
    38881 tcgatattca ggcggggtct ggcggtacgg aagcacagga ctgggcgagc atgcttgagc
    38941 gtatgtatct gcgctgggca gaatcgcgtg gtttcaaaac tgaaatcatc gaagagtcgg
    39001 aaggtgaagt ggcgggtatt aaatccgtga cgatcaaaat ctccggcgat tacgcttacg
    39061 gctggctgcg tacagaaact ggcgttcacc gcctggtgcg taaaagcccg tttgactccg
    39121 gcggtcgtcg ccacacgtcg ttcagctctg cgtttgttta tccggaagtt gatgatgata
    39181 ttgatatcga aatcaacccg gcggatctgc gcattgacgt ttatcgcgcg tccggcgcgg
    39241 gcggtcagca cgttaaccgt accgaatctg cggtgcgtat tacccacatc ccgaccggga
    39301 tcgtgaccca gtgccagaac gaccgttccc agcacaagaa caaagatcag gccatgaagc
    39361 agatgaaagc gaagctttat gaactggaga tgcagaagaa aaatgccgag aaacaggcga
    39421 tggaagataa caaatctgac atcggctggg gcagccagat tcgttcttat gtccttgatg
    39481 actcccgcat taaagatctg cgcaccgggg tagaaacccg caacacgcag gccgtgctgg
    39541 acggcagcct ggatcaattt atcgaagcaa gtttgaaagc agggttatga ggaaccaaca
    39601 tgtctgaaca acacgcacag ggcgctgacg cggtagtcga tcttaacaat gaactgaaaa
    39661 cgcgtcgtga gaagctggcg aacctgcgtg agcagggaat tgccttcccg aacgatttcc
    39721 gtcgcgatca tacctctgac caattgcacg cagaattcga cggtaaagag aacgaagaac
    39781 tggaagcgct gaacatcgaa gtcgccgttg ctggccgcat gatgacccgc cgtattatgg
    39841 gtaaagcgtc tttcattacc ctgcaggatg tgggtggccg cattcagctg tacgttgccc
    39901 gtgacgatct gccggaaggc gtttacaacg agcagttcaa aaaatgggac ctcggcgaca
    39961 tcctcggcgc gaaaggtaaa ctgttcaaaa ccaaaaccgg cgaactgtct atccactgta
    40021 ccgagctgcg tctgctgacc aaagcactgc gtccgctgcc ggataaattc cacggcttgc
    40081 aggatcagga agcgcgctat cgtcagcgtt atctggatct catctctaac gatgaatccc
    40141 gtaacacctt taaagtacgc tcgcagatcc tctctggtat tcgccagttc atggtgaacc
    40201 gcggctttat ggaagttgaa acgccgatga tgcaggtgat cccaggcggt gccgctgcgc
    40261 gtccgtttat cacccaccat aacgcgctgg atctcgacat gtacctgcgt atcgcgccgg
    40321 aactgtacct caagcgtctg gtggtcggtg gcttcgagcg tgtattcgaa atcaaccgta
    40381 acttccgtaa cgaaggtatt tccgtacgtc ataacccaga gttcaccatg atggaactct
    40441 acatggctta cgcagattac aaagatctga tcgagctgac cgaatcgctg ttccgtactc
    40501 tggcacagga tattctcggt aagacggaag tgacctacgg cgacgtgacg ctggacttcg
    40561 gtaaaccgtt cgaaaaactg accatgcgtg aagcgatcaa gaaatatcgc ccggaaaccg
    40621 acatggcgga tctggataac ttcgactctg cgaaagcgat tgctgaatct atcggtatcc
    40681 acgttgagaa gagctggggt ctgggccgta tcgttaccga gatcttcgaa gaagtggcag
    40741 aagcacatct gattcagccg accttcatta ctgaatatcc ggcagaagtt tctccgctgg
    40801 cgcgtcgtaa cgacattaac ccggaaatca cagaccgctt tgagttcttc atcggtggtc
    40861 gtgaaatcgg taacggcttt agcgagctga atgacgcgga agatcaggcg caacgcttcc
    40921 tggatcaggt tgccgcgaaa gacgcaggtg acgacgaagc gatgttctac gatgaagatt
    40981 acgttaccgc actggaacat ggcttaccgc cgacagcagg tctggggatt ggcatcgacc
    41041 gtatggtaat gctgttcacc aacagccata ccatccgcga cgttattctg ttcccggcga
    41101 tgcgtccggt gaaataagca ttacgttatg ctcacaaccc cggcaaatgt cggggttttt
    41161 tatttaagct gggtaaatgc agataatcgt attctggctt cgcgatttgt cgcctgcatc
    41221 accatccacg gactgaacgc ccacggcgtg gcatcaatac cgcgtaatac atcagctaaa
    41281 tcacaccatt gataatccat cacttcatca tcgttgatct gtaacgcact ggtggtgcgt
    41341 gcggcaaata ccggacacac ttcattttcc acaatgccac tcggatcggt ggcgcggtag
    41401 cgaaagtcag gatagataga ttcaggaggc gtaatttcca cgccaagctc ataacggcaa
    41461 cggcggatca ccgcgtcttc gctgctttct cccagttgtg ggtgcccaca aaccgagtta
    41521 gtccacacgc caggccacgc ttttttgctc agtgcgcggc gggtaactaa taattgcccc
    41581 ttggcattaa acagccaact ggagaacgcg agatgtaaga gggtgtctgc cgtgtgtgcg
    41641 gcatactttt ccagcgtacc cgtgggaact ccctgtgcat tcaataaaat gacgtgttcc
    41701 gtttgcataa tttctcacat gtaattctga tcactcatca ttatagataa ttgaagagtg
    41761 aaatgattaa ccaagagagc gtttatcgac aaccgcccgt tttgagcgga tgccgacagg
    41821 taaaaaccat ttaaaaaagt agcatcatat atttgaagcg actttcaatg gccattgcaa
    41881 cacagtcaga aatgacagga atagagagag taaaaagcaa caacagactg aatatagcgc
    41941 gcattaaaaa attcatatta ttctccatgc tcatttttct tcagcttgcg catcagaagc
    42001 cacatacgta ctgtcctgac ttttaaatct ttatcagaga tggattcctt caccaggtca
    42061 gcatgatgct gatagccatt aaagaagacg ttcagcacaa cggcgctaag ggttgcgagc
    42121 ataatgccgc tatgtagcag cggttgcagt acggccggta acttagaaaa gaaatcgtga
    42181 gagagcgtcg gagtcatgcc aacgccgaga ctgatcgcca caatatagag gttgtaacgg
    42241 ttggtggtgt agttacagcg cgacagaatt cgaatccctg tcgccagtac catgccgaac
    42301 atcactagac cagcgccgcc cagcacaaat tgcggaatgg aggctaccag caccgccatt
    42361 tttggcacca tgccaaacag gattaatata attcccgaag aaatacacac ccagcggcta
    42421 tgaacgcgca tcacgctaac caggccaacg ttttgagaaa atgacgtgtg ggggaagctg
    42481 ttaaacgtgc cgcctatcat tgtccctacg ccatcgacac gcagcccgcg aataatatcg
    42541 tgtgaagaga gtttacgacc gactatttca cccagtgcca ggaacatccc catcgactcg
    42601 ataaacacga tgattaacac ggcagtcatg gtcagaatgg aaacgggatc gaaaatcggc
    42661 ataccaaacg acatcggggt gacaatcgca aaccacgagg catcatgtag cccggataaa
    42721 ttgacttcat tcatcatcca cgaaagtaaa aagccaaata caatccccag taatacggcg
    42781 acgttggaca taaatccttt cgcatagcga gtaatgagca agataaaaat taagacggca
    42841 aaggagatac ctaaataaac gggattacca tattgcggat tccctttacc tccagcggcc
    42901 cagtcaatac ccacctgaat gatgctaagc ccaatagaag taataaccac accggtaacc
    42961 agtggcggga ataaaggcat caagcgacca ataagtggcg ctaataatgt ggtgataaaa
    43021 cccgcggcga tagtggcacc aaatatcccc agcaggccga tatccgggtt catcccaatg
    43081 gctatcattg gtgttacagc agcaaaggtc accgacataa tcaccggcag gcggatcccc
    43141 ataaagcggc cgataccgat acattgcaat aatgtgacga tcccgcagca aaagagatcc
    43201 gagctaatga gcatcgcaat agcatctttt gagaggccca gtcggtcacc aatcataaga
    43261 ggaacagcga ctgcacctgc gtacatgaca aggacgtgtt gcagaccgag tatgatcagc
    43321 tttcctggtg ataatatgcg gtcaacctca tcagttgggc ggtcttgccc agaagatgag
    43381 ggaagttggg aatctatggc gctcatcagt gtttctcctt tcgcccgcca gaataaaaag
    43441 caaccgttta cgttaatgaa actgtctaaa cacatgctcg tggagtgcca gtttttgcga
    43501 ggaaggggag ttagagacag gaattgcgga ggcaatcaca taatcgtgct taaaccacga
    43561 aaatgacaag agaacctgac aatggaatta tctgt
//
LOCUS       DBKEKI010000021        41530 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.021, whole genome shotgun sequence.
ACCESSION   DBKEKI010000021 DBKEKI010000000
VERSION     DBKEKI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.021"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            125..199
                     /locus_tag="ACUQPX_001085"
     tRNA            125..199
                     /locus_tag="ACUQPX_001085"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:157..159,aa:Gln,seq:ctg)
     gene            237..311
                     /locus_tag="ACUQPX_001086"
     tRNA            237..311
                     /locus_tag="ACUQPX_001086"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:269..271,aa:Gln,seq:ctg)
     gene            complement(465..1640)
                     /gene="ubiF"
                     /locus_tag="ACUQPX_001087"
     CDS             complement(465..1640)
                     /gene="ubiF"
                     /locus_tag="ACUQPX_001087"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HHV9055857.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVIPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1786..3210
                     /gene="miaB"
                     /locus_tag="ACUQPX_001088"
     CDS             1786..3210
                     /gene="miaB"
                     /locus_tag="ACUQPX_001088"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HHV9055858.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3363..4403
                     /locus_tag="ACUQPX_001089"
     CDS             3363..4403
                     /locus_tag="ACUQPX_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HHV9055859.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PKYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4400..4867
                     /gene="ybeY"
                     /locus_tag="ACUQPX_001090"
     CDS             4400..4867
                     /gene="ybeY"
                     /locus_tag="ACUQPX_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HHV9055860.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4957..5835
                     /gene="corC"
                     /locus_tag="ACUQPX_001091"
     CDS             4957..5835
                     /gene="corC"
                     /locus_tag="ACUQPX_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HHV9055861.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQKLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5860..7398
                     /gene="lnt"
                     /locus_tag="ACUQPX_001092"
     CDS             5860..7398
                     /gene="lnt"
                     /locus_tag="ACUQPX_001092"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HHV9055862.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAALALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7795..8703
                     /gene="gltI"
                     /locus_tag="ACUQPX_001093"
     CDS             7795..8703
                     /gene="gltI"
                     /locus_tag="ACUQPX_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HHV9055863.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8873..9613
                     /gene="gltJ"
                     /locus_tag="ACUQPX_001094"
     CDS             8873..9613
                     /gene="gltJ"
                     /locus_tag="ACUQPX_001094"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HHV9055864.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9613..10287
                     /gene="gltK"
                     /locus_tag="ACUQPX_001095"
     CDS             9613..10287
                     /gene="gltK"
                     /locus_tag="ACUQPX_001095"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HHV9055865.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            10287..11012
                     /gene="gltL"
                     /locus_tag="ACUQPX_001096"
     CDS             10287..11012
                     /gene="gltL"
                     /locus_tag="ACUQPX_001096"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HHV9055866.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            11130..12065
                     /gene="rihA"
                     /locus_tag="ACUQPX_001097"
     CDS             11130..12065
                     /gene="rihA"
                     /locus_tag="ACUQPX_001097"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HHV9055867.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELIAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            12149..13819
                     /gene="hscC"
                     /locus_tag="ACUQPX_001098"
     CDS             12149..13819
                     /gene="hscC"
                     /locus_tag="ACUQPX_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HHV9055868.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(13879..15330)
                     /gene="djlC"
                     /locus_tag="ACUQPX_001099"
     CDS             complement(13879..15330)
                     /gene="djlC"
                     /locus_tag="ACUQPX_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HHV9055869.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15327..16034)
                     /locus_tag="ACUQPX_001100"
                     /pseudo
     CDS             complement(15327..16034)
                     /locus_tag="ACUQPX_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            16136..16690
                     /locus_tag="ACUQPX_001101"
     CDS             16136..16690
                     /locus_tag="ACUQPX_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HHV9055870.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16700..18127)
                     /locus_tag="ACUQPX_001102"
     CDS             complement(16700..18127)
                     /locus_tag="ACUQPX_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HHV9055871.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(18124..18831)
                     /locus_tag="ACUQPX_001103"
     CDS             complement(18124..18831)
                     /locus_tag="ACUQPX_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HHV9055872.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHLSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ANTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            18995..19972
                     /gene="ybeQ"
                     /locus_tag="ACUQPX_001104"
     CDS             18995..19972
                     /gene="ybeQ"
                     /locus_tag="ACUQPX_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HHV9055873.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(20042..20524)
                     /locus_tag="ACUQPX_001105"
     CDS             complement(20042..20524)
                     /locus_tag="ACUQPX_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HHV9055874.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20759..23341
                     /gene="leuS"
                     /locus_tag="ACUQPX_001106"
     CDS             20759..23341
                     /gene="leuS"
                     /locus_tag="ACUQPX_001106"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HHV9055875.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMAERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23356..23937
                     /gene="lptE"
                     /locus_tag="ACUQPX_001107"
     CDS             23356..23937
                     /gene="lptE"
                     /locus_tag="ACUQPX_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HHV9055876.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            23937..24968
                     /gene="holA"
                     /locus_tag="ACUQPX_001108"
     CDS             23937..24968
                     /gene="holA"
                     /locus_tag="ACUQPX_001108"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HHV9055877.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24970..25611
                     /gene="nadD"
                     /locus_tag="ACUQPX_001109"
     CDS             24970..25611
                     /gene="nadD"
                     /locus_tag="ACUQPX_001109"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HHV9055878.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25635..26246
                     /locus_tag="ACUQPX_001110"
     CDS             25635..26246
                     /locus_tag="ACUQPX_001110"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HHV9055879.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNNRAIGVENA"
     gene            26506..26823
                     /gene="rsfS"
                     /locus_tag="ACUQPX_001111"
     CDS             26506..26823
                     /gene="rsfS"
                     /locus_tag="ACUQPX_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HHV9055880.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26827..27294
                     /gene="rlmH"
                     /locus_tag="ACUQPX_001112"
     CDS             26827..27294
                     /gene="rlmH"
                     /locus_tag="ACUQPX_001112"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HHV9055881.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27325..29226
                     /gene="mrdA"
                     /locus_tag="ACUQPX_001113"
     CDS             27325..29226
                     /gene="mrdA"
                     /locus_tag="ACUQPX_001113"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HHV9055882.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKIDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKQWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            29229..30341
                     /gene="mrdB"
                     /locus_tag="ACUQPX_001114"
                     /gene_synonym="rodA"
     CDS             29229..30341
                     /gene="mrdB"
                     /locus_tag="ACUQPX_001114"
                     /gene_synonym="rodA"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HHV9055883.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30352..31440
                     /gene="rlpA"
                     /locus_tag="ACUQPX_001115"
     CDS             30352..31440
                     /gene="rlpA"
                     /locus_tag="ACUQPX_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HHV9055884.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31579..32790
                     /gene="dacA"
                     /locus_tag="ACUQPX_001116"
     CDS             31579..32790
                     /gene="dacA"
                     /locus_tag="ACUQPX_001116"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HHV9055885.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALDLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32901..33164
                     /gene="ybeD"
                     /locus_tag="ACUQPX_001117"
     CDS             32901..33164
                     /gene="ybeD"
                     /locus_tag="ACUQPX_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HHV9055886.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            33265..33906
                     /gene="lipB"
                     /locus_tag="ACUQPX_001118"
     CDS             33265..33906
                     /gene="lipB"
                     /locus_tag="ACUQPX_001118"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HHV9055887.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            34165..35118
                     /locus_tag="ACUQPX_001119"
     CDS             34165..35118
                     /locus_tag="ACUQPX_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HHV9055888.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            35327..36292
                     /gene="lipA"
                     /locus_tag="ACUQPX_001120"
     CDS             35327..36292
                     /gene="lipA"
                     /locus_tag="ACUQPX_001120"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HHV9055889.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36393..36596)
                     /gene="tatE"
                     /locus_tag="ACUQPX_001121"
     CDS             complement(36393..36596)
                     /gene="tatE"
                     /locus_tag="ACUQPX_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HHV9055890.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36725..37513)
                     /locus_tag="ACUQPX_001122"
     CDS             complement(36725..37513)
                     /locus_tag="ACUQPX_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HHV9055891.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37606..37989
                     /gene="crcB"
                     /locus_tag="ACUQPX_001123"
     CDS             37606..37989
                     /gene="crcB"
                     /locus_tag="ACUQPX_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HHV9055892.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(38043..38252)
                     /gene="cspE"
                     /locus_tag="ACUQPX_001124"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(38043..38252)
                     /gene="cspE"
                     /locus_tag="ACUQPX_001124"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HHV9055893.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38427..38987)
                     /gene="pagP"
                     /locus_tag="ACUQPX_001125"
     CDS             complement(38427..38987)
                     /gene="pagP"
                     /locus_tag="ACUQPX_001125"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HHV9055894.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39575..40960
                     /gene="dcuC"
                     /locus_tag="ACUQPX_001126"
     CDS             39575..40960
                     /gene="dcuC"
                     /locus_tag="ACUQPX_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HHV9055895.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHNVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(41001..41342)
                     /locus_tag="ACUQPX_001127"
                     /pseudo
     CDS             complement(41001..41342)
                     /locus_tag="ACUQPX_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT        10412 a        10477 c        10354 g        10287 t
ORIGIN      
        1 gctacgtagc tcagttggtt agagcacatc actcataatg atggggtcac aggttcgaat
       61 cccgtcgtag ccaccaaatt ctgaatgtgt cgaatacgtt cggcaaattc aaaaaccaat
      121 ttgttggggt atcgccaagc ggtaaggcac cggattctga ttccggcatt ccgaggttcg
      181 aatcctcgta ccccagccaa tttattcaag acgcttacat cgtaagtgca cccagttggg
      241 gtatcgccaa gcggtaaggc accggattct gattccggca ttccgaggtt cgaatcctcg
      301 taccccagcc acattaaaaa agctcgcttc ggcgagcttt ttgcttttct gcgttcattc
      361 aatgtcgaat gcgatgttga cacgtcttat ccttcaatgt cggatgcgac gctgccgcgt
      421 cttatccgac ctacggttgg cacgcatccg gcaatgttgt aaggctacaa ccctaacgca
      481 tatttcagcg cctgacgttt caacacgcca gcacgctctg ccgccattaa cccgagatta
      541 cgcacaaaac gcagcggcgg cagattatta ctgaatccgg cataaaacag atccatacca
      601 ctttgcataa tgaagttatc cgccatgcgc cgcatctggt aacgcttgag gacaggataa
      661 ctggcccacg cttcaccgta gctgcgggca ttcaccagaa catcaatcag ggcatcgaca
      721 tcacgataac caagattcac cccctgcccc gccagcggat ggatggtatg cgcggcatcg
      781 cccaccagcg caagccccgg ctgcacatac tgcaacgcat gtcggcgcgt cagcggaaac
      841 gcaccagcgg caagcggtat aacgtaaccc agacgcgacg ggaaatgctt cgcgatttcc
      901 gcctggagtt gcgccatatt catattctgc aactggcgaa tacgcgccgg agagtcatac
      961 cacaccagcg atgcccagtt atcaaacaac ggcagaaacg cacgcggtcc gtccggggta
     1021 aattgctgcc aggtgctgtc gccgggatcg ttctcacact gtacgctaat caacatgcac
     1081 gactgcgcat actgccaggc atgaacgcca attcccgcca tctgccgcac ctgagaattt
     1141 gcgccgtcgg caccaatcac cagtttcgcg cggatcgttt caccgccctt cagttccagc
     1201 tcctgaagat cattatctcg atgtaatgca atcagcgagg ttggcacacg taacgttact
     1261 ttcggatgcg cttccagcgc ctgccacaac gcctgttgca ggactgtgtt ttccaccatg
     1321 tagccaagca acggcaactt taattcggct gcgtcaaata ccacatgtgc cgtttcccac
     1381 tcccaggttt ccagtctgcg gtaaggatgg caacgcatag cctgtactgc atcccagacc
     1441 cctaaccctt taagcaatga taccgaagcc gcgctgatcg ccgagatccg cacgtccggt
     1501 tggctgtcag cgacaaacgg tgcaggttct gcgtgctcga tcaccgttac cgaaaatccg
     1561 tgctgtgcca gccccagcgc cagtgcgccg ccgaccattc ctccgccgac aatggcaatt
     1621 tccgttggtt gatttgtcat ggtcaatcat cctgttagca atatgcttta agtttacagg
     1681 atttttaccc ccaagggctg ataacgctca tactggtcac aacggcagca aagcattaca
     1741 ctatgcgccc tgcattcctg gctactattt cgcaagagca agtcgatgac caaaaaactc
     1801 catattaaaa cctggggctg tcagatgaac gagtacgatt catcgaagat ggccgatctg
     1861 ctggatgcca cccacggcta tcaactgacc gacgtggcgg aagaagcgga tgtgctactg
     1921 ctgaacacct gctcaatccg cgagaaggct caggaaaaag tcttccatca gttgggtcgc
     1981 tggaaactgt taaaagagaa gaatccagac ctgattatcg gcgtcggtgg ctgcgtggca
     2041 tcgcaagaag gcgagcacat tcgccagcgc gcccactatg tcgatattat ttttgggccg
     2101 caaacgctgc accgtctgcc ggagatgatc aactccgtgc gcggcgaccg cagcccggtt
     2161 gtagatatca gcttcccgga aatcgagaag tttgaccgtc tgccggaacc gcgcgccgaa
     2221 gggccgaccg cgtttgtctc catcatggaa ggctgcaata aatattgcac ctactgcgtg
     2281 gtgccttaca cccgtggtga agaggtaagc cgtccgtctg acgatattct gtttgagatt
     2341 gcccagcttg cggctcaggg cgtgcgtgaa gtcaacctgc tcggtcagaa tgtgaatgcc
     2401 tggcgtggtg agaactacga cggcaccacc ggatcgtttg ccgatctgtt gcgtctggtt
     2461 gctgcgatcg acgggatcga tcgtattcgc tttaccacca gccatccgat cgaattcacc
     2521 gacgatatca tcgaagtgta ccgcgacacg cctgagctgg tgagcttcct gcatctgccg
     2581 gtacagagcg gttccgatcg cattctgaac ctgatgggac gtacccatac ggcgctggag
     2641 tacaaagcga tcatccgtaa actgcgtgcg gcgcgtccgg atattcagat cagttctgac
     2701 ttcattgttg gcttccctgg cgaaaccacc gaagacttcg agaaaacgat gaagctgatt
     2761 gccgacgtca atttcgacat gagctacagc tttatcttct ctgcacgtcc aggaacgcct
     2821 gccgccgata tggttgatga tgttccggaa gaagagaaga agcagcgtct gtatattctg
     2881 caagaacgta tcaatcagca agcgatggca tggagccgtc ggatgctcgg caccacccag
     2941 cgtattctgg tagaagggac ttcgcgtaag agcatcatgg agctttccgg gcgtaccgaa
     3001 aataaccgcg tggtcaactt cgagggcaca ccggatatga tcggtaaatt cgtcgatgta
     3061 gaaattaccg acgtctaccc gaactctctg cgcggtaaag tggtgcgaac tgaagatgaa
     3121 atgggtctgc gcatggcaga aacaccggag tcggtgattg cccgcacccg caaagaaaac
     3181 gaccttggcg tgggttatta tcagccgtaa ttctcaggcc tgccgttccg gtgggccttg
     3241 ttttttctct ctttatcccc atctttatcg caatgcttgc gcctttttcc tgtggcgtga
     3301 ataatttcct tataggccgg tgaatgttca gcttccggcg gcatacagat aagaggaacg
     3361 gtttgaacat agacactcgc gaaatcaccc tggagccagc agacaatgcg cgtctgttga
     3421 gcctgtgcgg cccgtttgat gacaacatca agcagctcga acgccgtctc ggcatcgaga
     3481 tcaatcgccg cgataaccac tttaaactga ccggccgtcc gatttgcgtc accgctgcgg
     3541 cagacattct gcgtagcctg tatgtcgata ctgccccgat gcgcggtcag attcaggata
     3601 tcgaaccgga acagatccac cttgcgatta aagaagcgcg ggtactggag caaagcgcgg
     3661 agagcgtgcc gaagtacggc aaagcggtca atatcaaaac taaacgcggc gtaattaagc
     3721 cgcgcacgcc aaaccaggcg cagtacatcg ccaatattct cgaccatgac attaccttcg
     3781 gcgttggccc ggcgggtacg ggtaaaacct acctggcagt ggctgcggca gttgatgccc
     3841 tggagcgtca ggaaattcgc cgtattctgc tgactcgtcc ggcggtcgaa gccggtgaga
     3901 aactgggctt cctgcctggc gatttaagcc agaaagtaga cccgtatctg cgcccactgt
     3961 acgacgcgct gtttgaaatg ctgggctttg agaaagtcga gaaactgatt gagcgcaacg
     4021 ttattgaagt cgcgccgctg gcctatatgc gtggtcgtac gctgaacgac gcgtttatca
     4081 ttctcgatga gagccagaac actaccatcg aacagatgaa gatgttcctg acccgtatcg
     4141 gttttaactc aaaagcggtt atcaccggcg acgtcacaca gatcgacctg ccgcgtaata
     4201 ctaaatcagg cttacgtcat gccattgaag tgctggccga tgtcgaagag atcagcttta
     4261 acttcttcca cagcgaagac gtggttcgtc acccggtggt ggcgcgtatc gttaacgctt
     4321 atgaagcctg ggaagaagca gaacaaaaac gtaaagcggc gctggcagca gaacgcaagc
     4381 gcgaagaaca ggaacaaaaa tgagtcaggt gatcctcgat ttacaactgg catgtgaaga
     4441 taattccggg ttaccggaag agagccagtt tcaaacatgg ctgaatgcgg tgatcccgca
     4501 gtttcaggaa gagtcggaag tgactattcg cgtggtcgat accgccgaaa gccacagtct
     4561 gaatctgacc tatcgcggta aggataagcc gaccaacgtg ctctccttcc cgtttgaagt
     4621 gccgcctggc atggagatgt cgctactggg cgatctggtt atctgccgtc aggtggttga
     4681 gaaggaagct caggagcaag gcaaaccact ggaggcgcac tgggcgcata tggtggtgca
     4741 cggcagtctg catttgttag gttacgatca cattgaagat gacgaagcag aagaaatgga
     4801 agccctcgaa acagagatta tgcttgctct gggctatgag gatccgtaca ttgccgagaa
     4861 agaataattt gccaggcatt tgactggcag acgtccccgc cgttggtgat taacgacggc
     4921 gttttgatta actaacatga gaacccatac gacgccatga gcgacgacaa ttcacacagt
     4981 agtgacacga taagcaacaa gaagggattt ttctccctgt tactcagcca acttttccac
     5041 ggtgaaccga aaaaccgtga cgaactgctg gcgctgatcc gtgattccgg gcagaacgac
     5101 cttatcgacg aagatacgcg cgatatgctc gaaggggtga tggacatcgc agaccaacgc
     5161 gttcgcgaca tcatgatccc ccgctcccag atgattaccc tgaaacgcaa ccagaagctg
     5221 gacgaatgcc ttgatgtcat catcgagtcc gcccactcac gtttcccggt gattagcgaa
     5281 gacaaagatc acattgaagg gattctgatg gcgaaagact tgctgccgtt tatgcgcagc
     5341 gatgctgaag ccttcagcat ggacaaagtg ttacgtcagg cggttgtcgt tcctgaaagt
     5401 aagcgcgtag accggatgct gaaagagttt cgctctcagc gttaccacat ggcgatcgtt
     5461 attgacgaat tcggtggggt ttccggtctg gtgaccattg aagacatcct ggaactgatt
     5521 gttggtgaga ttgaagacga gtatgacgaa gaagatgata tcgacttccg tcagctgagt
     5581 cgtcatacct ggaccgtgcg cgcactggct tccattgaag acttcaacga agcgttcggc
     5641 acccacttta gcgatgaaga agtcgacact atcggtggtc tggtgatgca ggcgtttggg
     5701 catcttccgg cgcgtggcga aactatcgac atcgacggtt accagttcaa agtggcgatg
     5761 gccgatagtc ggcgtattat tcaggttcat gtcaaaatcc cggatgactc accccagcca
     5821 aagctggatg aataaaaccg aaactggata gataactaca tggcttttgc ctcattaatt
     5881 gaacgccagc gcattcgcct gctgctggcg ttattattcg gtgcctgcgg aacgctggcc
     5941 ttctctcctt acgacgtctg gcctgcggcg attatttcgc tgatggggct tcaggcgttg
     6001 acctttaacc gtcgcccact ccagtctgcc gctattggct tttgctgggg atttggcctc
     6061 tttggcagcg gtattaactg ggtctatgtc agcatcgcga cctttggcgg gatgcctggc
     6121 ccggttaaca tcttcctggt ggtgctgctg gcggcgtatt tgtcgctgta taccggactg
     6181 tttgctggcg tgctgtcgcg tctgtggccg aaaaccacct ggctgcgcgt ggcgattgcc
     6241 gcccttgctc tctggcaagt gaccgagttt ctgcgcggtt gggtgctgac tggcttcccg
     6301 tggttacagt tcggctatag ccagattgat ggcccgttaa aagggctggc accgctaatg
     6361 ggcgtggaag ccattaactt cctgctgatg atggttagtg gcctgctggc actggcgttg
     6421 gtcaaacgca actggcgtcc gctggtggtg gccgtcgtgc tgtttgccct acccttcccg
     6481 ctgcgttaca tccaatggtt taccccgcaa ccggagaaaa ccattcaggt ttcgatggtt
     6541 cagggcgata ttccgcaatc gctgaaatgg gacgaaggcc agcttcttaa tacgctaaag
     6601 atttactaca acgcaacggc accgctgatg ggcaaatcat cgttgattat ctggccggag
     6661 tcggcgataa ccgatctgga aattaatcag caaccgttcc tcaaagcact ggacggtgag
     6721 ttgcgtgata aaggtagctc gctggtaacc gggattgtcg acgcgcgtct caataagcag
     6781 aaccgctacg atacctacaa caccatcatc acgctgggta aaggtgcacc gtacagctac
     6841 gaatcagccg atcgctataa caaaaaccat ctggtgccgt ttggcgagtt tgtcccgctg
     6901 gagtcgattc tgcgtccgtt agcaccgttc tttgatctgc cgatgtcgtc gttcagccgt
     6961 gggccatata tccagccgcc gctgtcggca aatggtattg agcttactgc ggctatttgc
     7021 tacgagatca ttctcggcga gcaagtgcgc gataacttcc gcccggatac cgactacctg
     7081 ctgactatct ccaacgatgc gtggtttggt aaatctattg gtccatggca acacttccag
     7141 atggcgcgaa tgcgtgcgct ggagctggcg cgcccactgt tgcgcagcac caacaacggc
     7201 attacggcgg tgattggccc gcagggtgag attcaggcga tgatcccgca gttcacccgc
     7261 gaggtgttaa ccactaacgt gacgccgacc accggactca caccatacgc acgtaccggc
     7321 aactggccgc tgtgggtgct gacggcactg tttggttttg ctgctgtgtt gatgagtctg
     7381 cgtcagcgac gtaaataatc ccttcttatc gtgccggaat acatcccata tttcggcacg
     7441 ctcaccggat gcggcatcag tgcattatcc ggataacaat attctcctat ccaactttgg
     7501 cacatctatt gctttgttat acaaggcaaa gcttgaacca gcatcagtac aacccggttg
     7561 caccacagca atccaccggt agcaccaaaa gagtgcattt taagaatttt gcttcagaat
     7621 ggtgcggtgc acctcgcaaa aacaaacaat aaaacatcaa tatctttaca tttagccaaa
     7681 ctaaatgtta cctaacaggc acaacactgc acaataaagt tgcagacgat aacaacacaa
     7741 acactcacaa cgggtatcca tgcgttctta acgcagaaga taaaggagtt ggatatgcaa
     7801 ttacgtaaac ctgccacagc aatcctcgcc ctggcgcttt ccgcaggact ggcacaggca
     7861 gatgacgccg ccccggcagc aggcagtacg ctggacaaaa tcgccaaaaa cggtgtgatt
     7921 gtcgtcggtc accgtgaatc ttcagtacct ttctcttatt acgacaatca gcaaaaagtg
     7981 gtgggttact cgcaggatta ctccaacgcc attgttgaag cagtgaaaaa gaaactcaac
     8041 aaaccggact tgcaggtaaa actgattccg attacctcac aaaaccgtat tccactgctg
     8101 caaaacggca ctttcgattt tgaatgtggt tctaccacca acaacgtcga acgccaaaaa
     8161 caggcggctt tctctgacac tattttcgtg gtcggtacgc gcctgttgac caaaaagggc
     8221 ggcgatatca aagattttgc cgacctgaaa ggcaaagccg tagtcgtcac ttccggcact
     8281 acctctgaag ttttgctcaa caaactgaat gaagagcaaa aaatgaatat gcgcatcatc
     8341 agcgccaaag atcacggtga ctctttccgc accctggaaa gcggtcgtgc cgttgccttt
     8401 atgatggatg acgctctgct ggccggtgaa cgtgcgaaag cgaagaaacc agacaactgg
     8461 gatatcgtcg gcaagccgca gtctcaggaa gcctacggtt gcatgttgcg taaagacgat
     8521 ccgcagttca aaaagctgat ggatgacact atcgctcagg tgcagacctc cggtgaagcg
     8581 gaaaaatggt ttgataaatg gttcaaaaat ccaattccgc cgaaaaacct gaacatgaat
     8641 ttcgaactgt cagacgaaat gaaagcactg ttcaaagaac cgaatgacaa ggcactgaac
     8701 taattacaag aaccaggggc ggaaattcca gccctctcga ttgttacgta gcacggacag
     8761 actatacgcc tgatggtcgt tccccatcgg gcctgaaaac cgcaatacgc tgggtaacaa
     8821 tcttcgaggg tagcagttaa cgctgctacc ctcttttttc tggagtagat ttatgtctat
     8881 agactggaac tggggtattt ttttacaaca agccccgttc ggcaatacca cctatcttgg
     8941 ttgggtctgg agtggttttc aggtgacgat cgctttgtcg atctgcgcct ggattatcgc
     9001 tttcctcgtc ggttcatttt tcggcatttt acgtaccgtt ccaaaccgtt ttctctccgg
     9061 tctgggtacg ttgtatgtcg aactgttccg caacgtgccg ttgattgtgc aattctttac
     9121 ctggtatctg gtgatcccgg agctgctgcc ggagaaaatc ggcatgtggt ttaaggccga
     9181 gctggatccc aatattcagt ttttcctttc atccatgctc tgcctggggc tgtttaccgc
     9241 cgcccgtgtt tgcgaacagg tacgcgccgc tattcagtcg ctaccgcgtg ggcagaagaa
     9301 tgccgcgctg gcgatgggcc tgacgctacc gcagacttat cgatacgtac tgctgcctaa
     9361 tgcttatcgc gttatcgtcc cgccgatgac ttccgagatg atgaacctgg tgaaaaactc
     9421 cgccatcgcc tcgaccatcg gtctggtgga tatggcggcg caggcgggta aattgctcga
     9481 ctactcggct cacgcctggg agtcgtttac cgccatcacg cttgcgtatg tgttgattaa
     9541 cgcttttatc atgctggtga tgacgctggt tgaacgtaaa gttcgcctgc ccggcaacat
     9601 ggggggcaaa taatgtacga gtttgactgg agttccattg ttccttccct gccatatctg
     9661 ctcgacgggc tggtgatcac cctgaaaatc accgtcacgg cagtcgtgat tggtattttg
     9721 tggggtacca tgctggcggt gatgcgcttg tccagcttcg cgcctgtttc gtggtttgcc
     9781 aaagcctacg ttaacgtatt ccgctcgatt cctttagtca tggttttgct gtggttttac
     9841 ttgattgtgc cgggatttct gcaaaatgtg ctgggattat cgccaaaaaa tgatattcgt
     9901 ttaatttcgg cgatggtggc gttttcgatg tttgaagcgg cctactattc agagattatc
     9961 cgtgccggta ttcaaagtat ttcccgtggt cagtcgagcg ccgcgctggc gttggggatg
    10021 actcactggc agtcgatgaa actgattatt ctgccgcagg ctttccgcgc gatggtgccg
    10081 ctgctgctca ctcagggcat cgtactgttc caggatacct cgctggtgta tgtgttatct
    10141 ctggccgatt tcttccgtac cgcctcaacc attggtgagc gtgatggtac gcaggtcgag
    10201 atgatcctgt ttgccggatt tgtttatttc gttattagcc ttagcgcgtc gttgttggtc
    10261 agctacttga aaagaaggac agcataatga ttaccctgaa aaatgtttca aaatggtatg
    10321 gtcactttca ggtgctgacc gactgctcaa ccgaagtgaa aaaaggcgaa gtggtggtgg
    10381 tttgcggccc gtctggttcc ggcaaatcaa cgctgattaa aaccgtcaac ggcctcgaac
    10441 cagtgcagca aggtgaaatc accgtcgatg gtatcgtggt taacgacaag aaaaccgatc
    10501 tggcaaagct gcgttcccgc gtcgggatgg tattccagca tttcgagctg ttccctcatc
    10561 tgtcgattat cgaaaacctg accctggcgc aggtgaaagt gcttaaacgc gataaagcgc
    10621 ccgctcgtga aaaagcattg aaattgctgg agcgcgtggg actttccgca catgccaata
    10681 aatttccggc gcagctttcc ggcggtcagc agcagcgtgt ggcaatcgct cgcgcattgt
    10741 gtatggatcc tattgcgatg ctgttcgacg aaccgacatc ggcgctggat ccggagatga
    10801 tcaacgaagt gctggacgtg atggtggaac tggcgaacga aggaatgacc atgatggtgg
    10861 tgacccacga aatgggcttt gcccgcaaag tggcgaatcg ggtgatcttt atggacgagg
    10921 gtaaaattgt cgaagactcg ccgaaagacg ctttcttcga tgatccgaaa tcggaccgcg
    10981 caaaagactt cctcgcgaaa atcctgcatt aatcactctg gcgcgcatac ccaattgcgc
    11041 gccatctcac gctttatcgt ttcgcccctc gtatctgtta catcctggcg ttacccttgt
    11101 cgcaaagaag cacacaacaa ggagcaacaa tggcactgcc aattctgtta gattgcgacc
    11161 caggtcatga tgacgctatc gcaatagttc tcgccctcgc ctcaccagag cttgatgtca
    11221 aagcaattac gtcttccgcc ggaaaccaga caccagaaaa aaccttacgc aatgttctgc
    11281 gtatgctgac cttgcttaat cgcaccgata ttccggtggc aggcggcgcg gtaaaaccgt
    11341 taatgcgtga gttgattatc gcggacaatg tgcacggcga aagcggtctc gacggcccgg
    11401 cattaccgga accgacgttc gcaccgcaaa actgtacggc ggtggagctg atagcgaaaa
    11461 cgctgcgcga aagtgcggaa cctgtcacga ttgtttctac cggaccgcaa actaacgttg
    11521 ccttgctgct caatagccat ccggaactgc atagcaaaat tgcccgtatc gtgattatgg
    11581 gcggcgcaat ggggcttggt aactggacgc ctgcggctga atttaatatt tacgttgacc
    11641 cggaagcggc agaaattgtc ttccagtcag ggatcccggt ggtgatggcc ggtctggatg
    11701 ttactcataa agcgcaaatc cacgttgaag acaccgagcg tttccgcgcg attggcaacc
    11761 ctgtttcaac cattgttgcc gaactgctgg atttcttcct cgaatatcat aaagacgaaa
    11821 aatggggctt tgtcggcgca ccgctgcatg acccatgcac catcgcctgg ctgttgaaac
    11881 ccgagttgtt taccactgtt gagcgctggg ttggcgtgga aacacagggg aaatataccc
    11941 agggtatgac ggttgttgat tattattatc tgactggcaa taaaccgaat gccactgtaa
    12001 ttgtcgatgt tgatcgtcag ggctttgttg atttactggc tgatcgtctg aaattttacg
    12061 cttaacactc gtcattgatt aagatcacca cgcgttacct gggtggtctt aatctattta
    12121 tctctttttc tttcaagata taaatgcaat ggataatgca gaactcgcca taggtatcga
    12181 tctcggcact accaatagtt taattgccgt ctggaaagac ggtgccgcgc aattaattcc
    12241 aaataagttc ggtgaatatt taacaccatc cataattagc atggatgaaa ataatcatat
    12301 tttagtcgga aaaccggctg tatcacggcg tacttcgcat ccggataaaa cggcagcgtt
    12361 atttaaacgt gcaatgggca gtaataccaa ctggcggtta ggcagcgaca catttaacgc
    12421 gccagaactg tcctctttgg tattacgctc attaaaagaa gatgccgaag aatttctgca
    12481 acgtccgatt aaagatgtgg tgatctccgt tccggcttat ttcagcgatg aacaacgcaa
    12541 gcatacccgt ttagcagcgg acttagccgg gttaaatgcg gtacgcttaa ttaatgaacc
    12601 cacagcagct gcgatggcgt atggcctgca tacccaacaa aatacccgtt cgctggtgtt
    12661 tgatctcggt ggcggcacgt ttgacgttac ggtgcttgag tacgccacgc cggtgattga
    12721 agttcacgcc tccgctggcg acaacttcct cggtggcgaa gattttaccc atatgttggt
    12781 cgatgacgtt ttaaagcgcg cggatgtcac aaggaccacg cttaacgaga gtgaactggc
    12841 agccttgtac gcctgtgtgg aagcggcaaa atgtagcaat caatcgccaa tgcacattcg
    12901 ctggcagtac caggatgaaa cgcgggaatg cgaattttac gagaacgaac tggaagattt
    12961 gtggttgccg ctgctcaatc gcttgcgagt gccgattgaa caggcgttgc gcgatgcgcg
    13021 tctgaagccg agtcaaatcg acagtctggt gctggttggc ggcgcgtcac aaatgccgct
    13081 ggtgcagcga atcgccgtgc gtctgtttgg caaattaccg tatcaaagtt acgatccgag
    13141 caccattgtc gcgctgggcg cagcaatcca ggccgcctgc cgcttacgca gtgaagatat
    13201 tgaagaagtg atcctcactg atatttgccc ttactcgttg ggcgttgagg ttaaccgcca
    13261 gggcgtttcc ggcattttct cgccgattat tgaacgaaac accactgtgc ccgtgtcgcg
    13321 tgtagaaact tattcaacca tgcacccgga acaggattca attacggtta acgtctatca
    13381 gggagaaaac cacaaagtta aaaacaacat tctggtggaa tccttcgatg tgccgttgaa
    13441 gaaaaccggg gcttatcagt cgattgatat tcgctttagt tatgatatca acgggttgct
    13501 tgaagttgac gtgcttctgg aagacggcag cgttaagtcc agagtgatta accacagccc
    13561 ggtaacattg agcgcgcagc agattgaaga gagtcggacg cggttatccg cattgaaaat
    13621 ttatccgcgc gatatgctca tcaatcgcac ctttaaagcc aaactggaag agttgtgggc
    13681 gcgggcgctg ggtgacgagc gagaagagat tggccgggtg atcaccgatt ttgatgcggc
    13741 gctgcagtca aacgatatgg cccgcgtcga tgaggtcagg cggcgggcga gcgtttattt
    13801 agccattgag acctcataat cacggctgcc ggatgcggcg taaatgctgc atccggcatc
    13861 ggcgcaaaaa acacatcgtc aactaaacaa catcgcgatt ttgccgagag cataacctat
    13921 aaccagcacc gttaaactga ttttgataat gctggtaatg ctctgtggtt tttcaccctc
    13981 atcatttttt tcttcatggt tttcaggtgt tggctcatcg acatcttcat cctccggctc
    14041 gagttttact ttcgtcaaca gcatccgggt gataatggcc tctaatggat gagcatctcc
    14101 gccttccagc cttgtattaa gacgggccag catctcttgt ttcggaaacg cttcaagtac
    14161 tacctgttcc gctacccgat acaaatccag taacggtgaa tcttcttctg tctcgctacc
    14221 gactctcgat ttccaccacg cccatgcttt gcgtttgaag aataacgact caataaacga
    14281 ttgacctaac ggcgataatt ttccgctatg taaaatttcc gcccataaca gccgcgtccg
    14341 ggcgctctca ccaggaacat atttcagcgg ttgaccttgc gcatctacca tgctccgcgc
    14401 ttcgataacc atcactaata acgcgaaata atccgggcaa tgacgcttgc tccactgcaa
    14461 caacaaatta tcagcttgtg tagatgggta actttcccag tacgcgccga gatcggcaag
    14521 caggctttcc ccctcgccgc agtacaaacg ttgtgcacac tggtaatacc gggcatcttc
    14581 gctttctggt tcttccgctt gccactgttt ggctacagga atgagttcca ccatacctgt
    14641 ttgcaccgag ctgtaccagc gcaacagttt gcgatgtaac tttgcatcat caggaatgaa
    14701 ccacggtcct tccatcgcca aaaacgcagt gacatagtca ggatggtaac ggcagcaacg
    14761 ttccagcgcg aagtaatatt caacagtctg gttctgtaac gcgacgggat attccagaag
    14821 gctaaggaaa tcgaaacaat caccagcctg aatggcctca agaaaagcct ccctttcttc
    14881 ctcatccatt ccttcgctgt cattaaacga atgccagttc aaacgctctg ccaacaaaga
    14941 agcacaattt aatgacagat atcgcgcttc tatagcgatt gcacacagcg gccagcgtaa
    15001 ttgatcgaca tcctccatgt tccaggtatt taattgctga ataaatttct gccaggcgga
    15061 gggctgaaaa cgatcacttt ctgaatccag taatgtcctg aatgcacgta gtatttcatg
    15121 ttcagcggtg gcatcttttt cttcatcatc tgcttcctcg acaggattta ccgccagtcg
    15181 cagcgcctct tcataggcct ggcgtaatgc tttaaacccc tgcggatcgg tttcgggatg
    15241 acacaacggt aagcgagcaa gataagcctg gcggataatg tctatttgcg tcgtgctttc
    15301 aatttcgagg atttgccaac atgttttcat aggtcgctca catccttaag cgttaccggg
    15361 cattcaggct ggacagttaa tggctgccag ggaagcgtgg catagaaacg gtcgcttccc
    15421 gctgccagct cttcgaacat ccgactcccg gcgctatcgt atttgtacag ggttagcgcc
    15481 tcgcgtagcg aagcgttgtc ttcgcactga gaagattgcc agtagagacg ccctagtgaa
    15541 taagcagaaa aatagtgcat ccagctgtga tagtaatggt gagctcgtgc atagacgcgc
    15601 gattgcagtc ataaactctc ttcttccgtc agccagttgt tgagcacacc gacccgtgac
    15661 agaaacccca ttcgaacata atcccaggct ttgattcccc cttctccaca gcacatagcg
    15721 gtttgggcaa cgaattgggt ataaatcaac cctctctccg agccaccagc aattaatgct
    15781 ttccactctt caggggaaga gcgttgccat gtcagatata agccagctaa atcattggca
    15841 tgaccatcat cagtcatctg ataaatagtc tgaattaaat catcacgatt atttattccc
    15901 caggatgatg agagatcgaa ttcttttatc ccggtataca tttttggcga gtcatgcgcg
    15961 ggtttgcaac tctgattaag tatttccatg ggcgcagaaa gcgcaaaaag aagatattgt
    16021 tcttccttat tcatttttta ataatccctt aagtgcatta actgactact tttttattat
    16081 tgatgagtat aaacttgacg ctttcaaaat aaaaagaaaa tcgaagcatt cacacatgaa
    16141 taaaaaatta atgtatatat tcgcaatttt tatagttgca gcaattacct gtattagcca
    16201 acccaagaaa acgacgttgc gtgataaagc catggtgaat tatgcctttg attatttaag
    16261 atcaccaggc agtctaccgt taaccacggc agccacggag ctttccgcga ttcatggtca
    16321 ctcaacatcg caatatcgcc ttggcgaatt ttatcttcat ggtagcgacg gtaaaccact
    16381 ggattataca caggcgagat actggtatga gcaatcagca gaacaggaaa atccgcgcgc
    16441 acaaagcaaa ctggggtgga tctacctgaa aggcctggga gtcaaacccg atacccgtaa
    16501 agcaattctc tggtataagg aagcagctga acaagggtat gctcatgctc aatatacttt
    16561 gggtttgatc tacagaaatg gcactggtat taatgttaac cattatgaat ctcaaaaatg
    16621 gttaaaactg gccgccaaac aacattacaa aaatgcggaa agattacttg ccggacttcc
    16681 cgcacattaa cgtcgactgt cacccgaaga gatggagtat tttccctatc aaaccggcaa
    16741 agataaaaat gtagaaaata attttgatga cgcccagaca gcccagcccg ccatcgtcaa
    16801 cgggcattgg ctgcggttgc ggctccggct cgacggtttc ctgaccggta aaattcgccg
    16861 cctgaattaa taatcgacga ataattaatg cttccagtgg acaacgatct tctgcatcaa
    16921 ggcggctcac aatcggcatt aatgattctt ctgtcggttg gctggcctgc actaattgtt
    16981 tggcaactaa atataatggc cataagggat gttctgtatc acgcgtgagt gagatccgcg
    17041 gcttacgtcc tttgtgcaaa accatctcga ccagtgaacg ccccagcgca gagagttttt
    17101 catcgctgag tatttcgtat aaatgaaaac gcgtacgggc gctgtcgccg gggacataaa
    17161 gtagcggttt tccctgatcg ttaaccagat cacgcgcttc aatcatcatc accactaatg
    17221 ggtaataatc gacccgatgc tgacggcacc attgcaacat taaagcatcc gcctgggtgg
    17281 agggatactc gcgccagtag taacaaagtt ctgccagcag gctttcgcct tctccacaat
    17341 agacgcgttg tgcgtattca taataacgcg catcttcatt atcaggttca gcttcccgcc
    17401 acgaccgggc gtaatctagg agttcgggga ttcccaggcg gcttgcgcta taccagcgga
    17461 gtaaatttct gtgcagtttt gcatcatcgg ggataatcac tgcgcgatgt tgcgctaaat
    17521 acaatgtcag ccattctgga tgcgatgacc aaatccgagc gaacatttgg taaaaatcga
    17581 tggtgtcatt ttgcacggca acgggcagat gcagaatggt ctggaagtta aaaacattcc
    17641 ccttcgcaat ggcatataaa aaggattcca gttcttcttc gtctatttcc ccgacgtttt
    17701 cctcctgcaa ccaccgcaat ctttctgcta ataacaccac gcactcgaag gataaatggg
    17761 cagtgttcat ggcgattgtg cacagcgacc agcgtaattc atcaatctct tccatcgagc
    17821 aataatttaa ttgctgaatg aatcgctgcc aggcggaggg cagaaaacgt tcgctatcag
    17881 aggcaagtaa cgcgcgaaag gcgagcagaa tttcatgttc tgctacctca tattcttctg
    17941 gttgccaaac agatttagca ggcgactgcg caatccgtag cgcctcctca tacgcctgac
    18001 gaagttgttt aaaaccctgc ggatcggttt ctggatgaaa ggacggtaac agcgccagat
    18061 aagcgcggcg gataatatcg acgtcagtcg tttcctctat atcgaggatc ttccagcaat
    18121 ttttcataag tcgctcatat ccttaagcgt ttccgggcat gtggggtagt cagccagtgg
    18181 ttgccaggac aaggtagggt aatacgcatc atcactggca aataattgat aaaacatatc
    18241 attgccggaa tcgtcgtatt ctttacgcgc taagaattcg cgtaataaag gcagatgatc
    18301 atcttcggga gactgccaat attgccgacc aaaggtataa ccggcaaagt attgccgcca
    18361 gttactataa taacgtagag cccgtagatg aatacgcgac tggatccata gactttcttc
    18421 ttcactcaac cagttattaa gtacgcccat tcgacttaaa aaacccatcc ggacataatc
    18481 ccatgcttta attcctccct caccacagca ttcggctgtg ttggcgacaa attgcgcata
    18541 agcctgacct tgctcattta attcagcaag atagtcacgc cattcacatg gagaataacg
    18601 aaaccagcgg tgataaaacc ctgccaaccg agcggcatga ccgttctcga tcattcggtg
    18661 aattgtgcgc agcaggtcat cacggtcatc aattccccat gaggaagaaa ggtcaaaaaa
    18721 gcatgtacct aaatacattt tgggggataa atgcgaaggt aaacagcatt cattgcgtat
    18781 ttccatcgga gtggaaagtg caaacaatat tttttgcgac cccatgtcca tataatttat
    18841 ccttaaacat tcatttaatg aatgtaaact acatttcgag actcagttat ttatactttt
    18901 ctgacgcaac ataatacgca taaaataagg cttacgcaaa aatggaacac ataaaaatat
    18961 aatatacttt atctacaacc ataaggaaat gataatgatt tttacgtcaa gttgctgcga
    19021 taatttatca atagatgaga ttatcgaacg tgctgaaaaa ggcgattgcg aggctcaata
    19081 tattgttggg ttttattata atcgcgatag cgcaattgac tctccggacg acgaaaaggc
    19141 cttttactgg ctgaagctgg ccgctgagca aggtcattgt gaagcacagt attccttagg
    19201 gcaaaagtat accgaggata aaagccgtca taaagataat gagcaagcca tcttctggct
    19261 gaaaaaagct gccctacaag gccatacttt cgcttccaac gcccttggct ggatactgga
    19321 tcgtggagaa gaccccaact ataaagaagc ggttacctgg tatcagatag ccgcggagag
    19381 cggaatgtct tatgcgcaaa ataatcttgg atggatgtac agaaatggca acggagtcgc
    19441 acaagactat acgctggcat ttttctggta caaacaagct gcattacaag gccatagtta
    19501 cgcacaagac aatctggccg atctttataa agacggagaa ggcgttgctc aaaacaagac
    19561 actcgccgca ttctggtatt tgaaaagcgc acagcagggt aatcggcacg cccagtttca
    19621 aattgcatgg gattataacg ctggcgaagg ggtggaccag gactataagc aagcgatgta
    19681 ctggtatctg aaggctgccg ctcaggaaag cgtcggcgct tacgtcaaca tcggttatat
    19741 gtataaacac ggacaaggcg ttgagaaaga ttatcaggct gcctttgaat ggtttacgaa
    19801 agccgctgaa tgcaatgacg ccactgcctg gtataacctg gccattatgt atcactacgg
    19861 agaaggaaga cctgtcgatc tccgacaggc tctcgacctg tatcgtaaag ttcagtcatc
    19921 cggaaccaga gatgtcagtc aggaaattcg tgagactgaa gatttactgt agaaataaaa
    19981 acaagggccg gggaatttca ttctgcccat attctcttta ttgctgtgcg aaatcagact
    20041 attacggctc aaacgggcgt ctctggaact ggtcataacc acatttcggg catagcgtca
    20101 gcacttccgg tgtgtagatc gggagatgga agtgacattt ctcgcagacc agatttccca
    20161 gcccgaccac ttctccgctg tgataaaccc catgatgatt gaggtcctgg aaaacttcgc
    20221 gccattcaag ctgcgtttta tcggtgatgt ctgccagctc ctgccacaag ctttctttaa
    20281 tcacccgcat aaagacgctg tcagattctt ctttcaggct ctcttcatag ctcatggcga
    20341 actcttccag gtcacgtctg acagctcgcg tcagctcatc gatctcggtt cgcgttaact
    20401 cccctgtttt tattacgcgc tcgcgcgcct gttccaccag tgcgtcgata tcacgttcgc
    20461 cattgcgcag gcgttcgttc agtgacgcga ccagttcacg gtaatattga gcaaccttgt
    20521 tcatcgtttc atctcccggg tcgttaactg actataataa tagacgttct ttgttccttg
    20581 ctttgctaat acgcccacaa actctgtaaa ttaatgtacg aataatccgg gcatcatttc
    20641 aacgtcgagt gcgcgaaagg ctgttttgac gcggacgttt gggctatgct atgcggatct
    20701 gaaaaaccac ctcaacgcta catttgtagc cgtattgaaa acaggaccac tggctgccat
    20761 gcaagagcaa taccgcccgg aagagataga atccaaagta cagcttcatt gggatgagaa
    20821 gcgcacattt gaagtaaccg aagacgagag caaagagaag tattactgcc tgtctatgct
    20881 tccctatcct tctggtcgac tacacatggg ccacgtacgt aactacacca tcggtgacgt
    20941 gatcgcccgt taccagcgca tgctgggcaa aaacgttctg cagccgatcg gctgggacgc
    21001 gtttggtctg cctgcggaag gcgcggcggt gaaaaacaac accgctccgg caccgtggac
    21061 gtacgacaac atcgcgtata tgaaaaacca gctcaaaatg ctgggctttg gttatgactg
    21121 gagccgcgag ctggcaacct gtacgccgga atactaccgt tgggaacaga aattcttcac
    21181 cgagctgtat aaaaaaggcc tggtatataa aaagacttct gcggtcaact ggtgcccgaa
    21241 cgaccagacc gtactggcga acgaacaagt tatcgacggc tgctgctggc gctgcgatac
    21301 caaagttgaa cgtaaagaga tcccgcagtg gtttatcaaa atcactgctt acgctgacga
    21361 gctgctcaac gatctggata aactggatca ctggccagac accgttaaaa ccatgcagcg
    21421 taactggatc ggtcgttccg aaggcgtgga gatcaccttc aacgttaacg actatgacaa
    21481 cacgctgacc gtttacacta cccgcccgga cacctttatg ggttgtacct acctggcggt
    21541 agctgcgggt catccgctgg cgcagaaagc ggcggaaaat aatcctgaac tggcggcctt
    21601 tattgacgaa tgccgtaaca ccaaagttgc cgaagctgaa atggcgacga tggagaaaaa
    21661 aggcgtcgat actggcttta aagctgttca cccattaacg ggcgaagaaa ttcccgtttg
    21721 ggcagcaaac ttcgtattga tggagtacgg cacgggcgca gttatggcgg ttccgggtca
    21781 cgaccagcgc gactacgagt ttgcctctaa atacggcctg aacatcaaac cggttatcct
    21841 agcggctgac ggctctgagc cagatctttc tcagcaagcc ctgactgaaa aaggcgtgct
    21901 gttcaactct ggcgagttca acggtcttga ccatgaagcg gccttcaacg ccatcgccga
    21961 taaactgact gcgatgggcg ttggcgagcg taaagtgaac taccgcctgc gcgactgggg
    22021 tgtttctcgt cagcgttact ggggcgcgcc gattccgatg gtgacgctgg aagacggtac
    22081 cgtaatgcca accccggacg accagctgcc ggtgatcctg ccggaagatg tggtgatgga
    22141 cggcattacc agcccgatta aagcagatcc ggagtgggca aaaactaccg ttaacggtat
    22201 gccagcgctg cgtgaaaccg acactttcga cacctttatg gagtcctcct ggtactatgc
    22261 gcgctacact tgcccagagt acaaagaagg tatgctggat tccgaagcgg ctaactactg
    22321 gctgccggtg gatatctaca ttggtggtat cgaacacgcc attatgcacc tgctctactt
    22381 ccgcttcttc cacaaactga tgcgtgatgc aggcatggtg aactctgacg aaccagcgaa
    22441 acagttgctg tgtcagggta tggtgctggc agatgccttc tactatgttg gcgaaaacgg
    22501 cgaacgtaac tgggtttccc cggttgatgc tatcgttgaa cgtgacgaga aaggccgtat
    22561 cgtgaaagcg aaagatgcgg caggccatga actggtttat accggcatga gcaaaatgtc
    22621 caagtcgaag aacaacggta tcgacccgca ggtgatggct gaacgttacg gcgcggacac
    22681 cgttcgtctg tttatgatgt ttgcttctcc ggctgatatg actctcgaat ggcaggaatc
    22741 cggtgtggaa ggggctaacc gcttcctgaa acgtgtctgg aaactggtgt acgagcacac
    22801 agcaaaaggt gatgttgcgg cactgaacgt tgatgcgctg actgaagatc agaaagcgct
    22861 gcgtcgcgat gtgcataaaa ctatcgctaa agtgaccgat gatatcggcc gtcgtcagac
    22921 cttcaacacc gcaattgcgg cgattatgga gctgatgaac aaactggcga aagcaccgac
    22981 cgatggcgag caggatcgcg ctctgatgca ggaagcactg ctggccgttg tccgtatgct
    23041 caacccgttc accccgcaca tctgcttcac gctgtggcag gaactcaaag gcgaaggcga
    23101 tatcgacaac gcgccgtggc cggttgctga cgaaaaagcg atggtggaag actccacgct
    23161 ggtcgtggtg caggttaacg gtaaagtccg tgccaaaatc accgttccgg tggacgcaac
    23221 ggaagaacag gttcgcgaac gtgctggcca ggaacatctg gtagcaaaat atcttgatgg
    23281 cgttactgta cgtaaagtga tttacgtacc aggtaaactc ctcaatctgg tcgttggcta
    23341 agcgcgggag gatgcgtgcg atatctggca acattgttgt tatctctggc ggtgttaatc
    23401 accgccgggt gtggctggca tctgcgtgac accacgcagg ttccttccac tatgaaggtc
    23461 atgatcctgg actcaggcga tccgaacggg ccattaagcc gtgcggtgcg taaccagtta
    23521 cgtctgaatg gtgtcgagtt gcttgataaa gaaaccacgc gtaaggacgt tccatccctg
    23581 cgtttgggta aagtgagcat cgcgaaagat accgcatcgg tattccgtaa cggtcaaaca
    23641 gcagagtatc agatgatcat gacggtgaat gcgaccgtgt tgatccccgg ccgtgatatc
    23701 tacccgatta gcgccaaagt cttccgttca ttcttcgata acccgcaaat ggcgttagcg
    23761 aaagataacg aacaagacat gatcgtaaaa gagatgtacg accgtgctgc cgaacagctg
    23821 attcgtaagc tgccaagcat ccgtgctgcg gatattcgtt ccgacgaaga acagacgtcg
    23881 accacaacgg atactccggc aacgcctgca cgcgtctcca ccacgctggg taactgatga
    23941 ttcggttgta cccggaacaa ctccgcgcgc agctcaatga agggctgcgc gcggcgtatc
    24001 ttttacttgg taacgatcct ctgttattgc aggaaagcca ggacgctgtt cgccaggtag
    24061 ctgcggcaca aggattcgaa gaacaccaca ctttttccat tgatcccaac actgactgga
    24121 atgcgatctt ttcgttatgc caggctatga gtctgtttgc cagtcgacaa acgctattgc
    24181 tgttgttacc agaaaacgga ccgaatgcgg cgatcaatga acaacttctc acactcaccg
    24241 gacttctgca tgacgacctg ctgttgatcg tccgcggtaa taaattaagc aaagcgcaag
    24301 aaaatgccgc ctggtttact gctcttgcga atcgcagcgt gcaggtgacc tgtcagacac
    24361 cggagcaggc tcagcttccc cgctgggttg ctgtgcgcgc aaaacagctc aacttagaac
    24421 tggatgacgc ggcgaatcag gtgctctgct actgttatga aggtaacctg ctggcgctgg
    24481 ctcaggcact ggagcgttta tcgctgctct ggccagacgg caaactgaca ttaccgcgcg
    24541 ttgaacaggc ggtgaatgat gccgcgcatt tcaccccttt tcattgggtt gatgctttgt
    24601 tgatgggaaa aagtaagcgc gcgttgcata ttcttcagca actgcgtctg gaaggcagcg
    24661 agccggttat tttgttgcgc acattacaac gtgaactgtt gttactggtt aacctgaaac
    24721 gccagtctgc ccatacgcca ctgcgtgcgt tgtttgataa gcatcgggta tggcagaacc
    24781 gccggggcat gatgggcgag gcgttaaatc gcttaagtca gccgcagtta cgtcaggccg
    24841 tgcaactcct gacacgaacg gaactcaccc tcaaacaaga ttacggtcag tcagtgtggg
    24901 cagagctgga agggttatct cttctgttgt gccataaacc cctggcggac gtatttatcg
    24961 acggttgata tgaaatcttt acaggctctg tttggcggca cctttgatcc ggtgcactat
    25021 ggtcatctaa aacccgtgga aacgctggcg aatttgattg gtctgacgcg ggtcacaatc
    25081 atccctaata atgttcctcc gcatcgtccc cagccggaag cgaacagcgt gcagcgtaaa
    25141 cacatgcttg aactggcgat tgccgacaag ccattattta ctcttgatga acgcgagcta
    25201 aagcgcaatg ccccctctta cactgcgcaa acactgaaag agtggcggca ggaacaagga
    25261 ccggacgtgc cgctggcgtt tattattggt caggattcat tgctgacctt tccgacctgg
    25321 tacgaatacg aaacgatact cgacaatgca catttgatcg tctgtcggcg tccaggttac
    25381 ccacttgaaa tggcgcaacc gcaataccag caatggctgg aagatcattt gacacataac
    25441 ccggaagatc ttcaccttca gcctgccggt aaaatttatc tggctgaaac gccgtggttt
    25501 aacatctcgg cgaccatcat ccgcgaacgt ttgcaaaacg gtgaatcgtg tgaggattta
    25561 ttgccggaac cggtattgac ttacattaac caacaaggct tgtatcgctg atacctgtcg
    25621 ttacggaggg catgatgcga ctgtggttaa ttcgtcatgg cgaaacacaa gcgaatgtcg
    25681 atggtctgta cagcggtcat gcgcccacac ccctgaccgc acgcggtatt gagcaagcgc
    25741 aaaatctgca tacactacta gatgatgttt cctttgacct ggttttatgc agtgaactgg
    25801 aacgggcaca gcataccgcg cgactggttc tcagtgaccg ccagctcccc gttcatatca
    25861 tccctgaact caacgaaatg ttttttggcg actgggagat gcgacatcat cgcgacctca
    25921 tgcaagaaga tgccgaaaac tatagcgcgt ggtgcaatga ctggcagcat gctatcccca
    25981 cgaacggtga aggatttcag gcattttcgc aacgtgtgga acgctttatc gcaaggctta
    26041 gtgaatatca gcactatcag aatattttaa tcgtcagcca tcagggcgta ctgagtctgt
    26101 taattgctcg tttaattggc atgcctgccg agtctatgtg gcattttcgc gttgaccagg
    26161 ggtgttggag cgccattgat atcaaccaaa aattcgcgac gctacgcgtc ctcaataacc
    26221 gtgccatcgg ggtcgagaat gcatgacttt tctgtttttt tacgggtaag ccgcaacgac
    26281 cattgacagc cccgggcagg ctgatattct ccgcagccag actttttccg ccagacacga
    26341 ctttgtagaa attgttttac aaaaatggcg atgcaatctg cggcgcgggg tgggatgata
    26401 gcccactttc gaaagccgat tcggcgacaa ttgtcccgaa atcgcctctg gttcaggtat
    26461 actgacagac catttttatc tatttgattc acccaggggg aaaacttgca gggtaaagca
    26521 ctccaggatt ttgttatcga caaaattgat gacctcaaag gtcaggacat catcgcctta
    26581 gacgttcaag gcaaatccag catcaccgac tgcatgatca tctgtacggg tacgtccagc
    26641 cgtcatgtta tgtccattgc tgaccacgtt gtgcaggagt ctcgcgcagc gggcctgtta
    26701 ccgctcggcg tagaaggtga aaacagcgcc gactggattg tcgtggattt gggcgatgtg
    26761 attgtccatg tcatgcagga agagagccgt cgcctgtatg aactggaaaa actctggagt
    26821 taatgcgtga agctgcaact tgtcgccgtg ggaacgaaaa tgccggactg ggtacaaacc
    26881 ggttttaccg agtacctgcg tcgttttccg aaagatatgc ccttcgagct gattgaaatt
    26941 ccggccggca aacgcggcaa gaatgcggac atcaagcgca tactcgacaa agagggtgag
    27001 cagatgttgg cggccgcagg caaaaaccgc attgtcaccc tcgatattcc aggcaagccc
    27061 tgggatacgc cgcagttagc cgctgagctg gaacgctgga agctggatgg tcgcgacgta
    27121 agtctactga ttggcgggcc tgaagggttg tcgcctgcct gtaaagcggc ggctgagcag
    27181 agctggtcgc tgtcggcgct taccctcccc catccgctgg ttcgcgtgct ggtcgcagag
    27241 agtctgtacc gggcgtggag catcaccacc aaccatcctt atcaccgtga gtgataaggg
    27301 agctttgagt agaaaacgca gcggatgaaa ctacagaact cttttcgcga ctatacggca
    27361 gagtccgcgc tgtttgtgcg ccgggcgctg gtcgcctttt tggggatttt gctgctgacc
    27421 ggcgtgctta tcgccaacct gtataatctg caaattgttc gctttaccga ctaccagacc
    27481 cgctctaatg aaaaccgcat taagctggtg cctatcgcgc ccagccgcgg cattatctac
    27541 gaccgtaacg gtatccctct ggccctcaac cgcactatct accagataga aatgatgccg
    27601 gagaaaatcg ataacgtgca gcaaacgctg gacgctttgc gcagcgtggt agatctgacc
    27661 gatgacgata ttgctgcatt ccgaaaagag cgcgcacgtt cacaccgttt cacctctatt
    27721 ccggtgaaaa ctaacctgac cgaagtacaa gtagctcgct ttgccgtcaa tcagtaccgt
    27781 tttccgggtg tcgaagttaa aggctataaa cgtcgttact atccttacgg ttcggcgttg
    27841 acccacgtca tcggctatgt gtcgaaaatc aacgataaag acgtcgaacg cctgaataat
    27901 gacggcaaac tggccaacta tgcggcaacg catgatattg gtaagctggg cattgagcgt
    27961 tactatgaag acgtgctgca cggtcagacc ggttatgaag aggttgaagt taacaaccgt
    28021 gggcgtgtta tccgccagtt aaaagaagta ccaccgcaag ccggacacga tatttacctg
    28081 acgctggatc tcaaactcca gcaatatatt gaaacgctgc tggcgggtag ccgcgcagct
    28141 gtggttgtta ccgatccgcg tacaggtggg gtgctggcgc tggtttccac gcctagttat
    28201 gacccaaacc tgtttgttga cggtatctcc agcaaagatt attccgcctt gctgaacgac
    28261 ccgaatacac cgctggtgaa ccgcgccaca cagggggttt atcctcccgc gtctacagtt
    28321 aaaccctatg tggcggtttc ggcattaagc gccggggtga tcacgcgcaa tacgacgctg
    28381 tttgacccag gctggtggca actaccaggg tcggaaaaac gttatcgtga ctggaaaaaa
    28441 tggggccacg ggcgtctgaa tgtcacaaga tcgctggaag aatctgcgga taccttcttc
    28501 tatcaggtgg cctacgatat ggggatcgat cgcctctccg aatggatggg taaattcggt
    28561 tatggtcatt acaccggtat cgacctggcg gaagaacgtt ccggcaacat gcctacccgc
    28621 gaatggaaac agaaacgctt taaaaaacag tggtatcagg gtgacaccat tccggttggt
    28681 atcggccagg gttactggac agcgacccca atccagatga gtaaggcact gatgatcctg
    28741 attaatgacg gtatcgtgaa ggttccgcat ttgctgatga gcaccgccga agacggcaaa
    28801 caggtgccat gggtacaacc gcatgaaccg cccgtcggcg atattcattc cggttactgg
    28861 gagctggcga aagacggtat gtacggtgtt gctaaccgcc ctaatggtac ggcgcataaa
    28921 tactttgcca gcgcaccgta caaaattgcg gcgaaatctg gtaccgctca ggtcttcggt
    28981 ctgaaagcga acgaaaccta taatgcgcac aaaattgccg agcgtttacg tgaccacaaa
    29041 ctgatgaccg cctttgcgcc atacaacaat ccgcaagtgg ctgtcgccat gattctggag
    29101 aacggcggcg cgggtccggc ggttggtacg ctgatgcgcc agatcctcga ccacattatg
    29161 ctgggtgata acaacaccga tctgcctgcg gaaaatccag cggttgccgc agcggaggac
    29221 cattaatcat gacggataat ccgaataaaa aaacattctg ggataaagtc catctcgatc
    29281 ccacaatgct gctgatttta ctggcattgc tggtttacag cgccctggtt atctggagcg
    29341 ccagcggtca ggatattggc atgatggagc gtaaaatcgg ccaaatcgcg atgggtctgg
    29401 tcatcatggt ggtgatggcg caaattcctc cacgcgttta tgaaggctgg gccccctatc
    29461 tctatatcat ctgtattatt ttgctggtgg cggtagatgc tttcggagcc atctctaaag
    29521 gcgctcaacg ctggctggac ctcggtattg ttcgttttca gccgtcggaa attgccaaaa
    29581 tagccgtacc actgatggtt gcgcgcttta tcaaccgcga cgtttgcccg ccatcgttga
    29641 agaacactgg catcgcgctg gtgctgatat ttatgcccac gctgctggtg gctgcacagc
    29701 ctgacctagg aacatcaatc ctcgttgcgc tttccggtct gtttgtactg ttcctctctg
    29761 gccttagctg gcgtctgatt ggcgtcgcag tagtgctggt agcggcgttc attccgattc
    29821 tgtggttctt cctgatgcat gattaccagc gccagcgcgt aatgatgctc ctggacccgg
    29881 aatcagaccc actcggcgcg ggctatcaca ttattcagtc taaaattgct attggctccg
    29941 gcggattacg cggcaaaggc tggctgcacg gcactcagtc acagcttgaa tttctccccg
    30001 aacgccatac tgactttatc ttcgcggtac tggcggaaga gctgggatta gtgggcattc
    30061 tgattctgct cgctctctac attctgctga tcatgcgcgg gctgtggata gccgccagag
    30121 cgcaaaccac ctttggtcgt gtcatggctg gtggcttaat gctgatatta ttcgtttatg
    30181 tcttcgtaaa tattggtatg gtaagcggta ttctgccggt tgtaggggtt ccgctcccac
    30241 tggtcagtta tggaggatcg gcgctaattg tgctgatggc tgggttcggg attgtaatgt
    30301 caatccacac ccacaggaaa atgttgtcga aaagcgtgta agaggtgcgc aatgcgtaag
    30361 cagtggctcg ggatctgcat cgcggcagga atgctcgcgg catgtacaag cgatgatggt
    30421 cagcaacaga cggtaagtgt accgcagcct gcggtatgta acggccccat agttgaaatt
    30481 agcggggcgg acccgcgttt cgaaccactg aacgcgacgg caaatcagga ttaccagcgc
    30541 gacggtaaaa gctacaaaat cgtgcaggat ccgtctcgat ttagccaggc gggactggcg
    30601 gcaatctatg atgccgaacc aggcagtaac ctgacggcct ctggcgaagc tttcgatccg
    30661 acaaagctga cggcggccca tccaacgctt ccgatcccca gctacgccag aatcactaac
    30721 ctggctaacg ggcgaatgat cgtggtgcgc attaatgatc gcggccctta cggcaacgac
    30781 cgcgttattt cgctttctcg cgcggcagct gaccgtctta acacgtcaaa caacaccaaa
    30841 gttcgtatcg atccgattat tgtcgcccag gatggttcgc tttctggtcc tggtatggct
    30901 tgtaccacag tcgccaaaca gacttacgcc ctgcctgcac ctcccgattt aagcggtggc
    30961 gcgggaacaa gttcagtgtc tggcccgcag ggtgacattc ttccggtcag taattcgacg
    31021 ctaaaaagcg aagatccgac cggcgcgccg gtaaccagta gcggtttcct cggcgctcca
    31081 acgaccttag cgcctggtgt actggaaggc agcgaaccga cgcctgcgcc acagcccgtt
    31141 gttacagctc cgtcgacaac gcctgcaacc tcgcctgcaa tggtgacacc gcaagccgcc
    31201 tcgcaaagcg ccagcggcaa ctttatggtg caagtcgggg ccgtaagcga tcaggctcgt
    31261 gcgcaacagt accaacagca actgggacag aagttcggcg tccccggtcg cgtaactcaa
    31321 aatggcgcgg tctggcggat ccagcttggc ccattcgcca gcaaagccga agccagtacc
    31381 ttgcagcaac gtttgcaaac cgaagcccaa ttacagtcat ttattactac cgcgcagtag
    31441 cgtaaagcag gcatctgatg tgtcaatttc tgtaaatgac gttaacaaag tcacgcggaa
    31501 agtcagatgc ctgccggtag tgcatttgct atagtagggc acttttttaa ttccatcacg
    31561 gatgtcgtag ttcagaccat gaataccatt ttttccgctc gtatcatgaa gcgcctggcg
    31621 ctcaccacgg ctctttgcac agcctttatc tctgctgcac atgccgatga cctgaatatc
    31681 aaaactatga tcccgggtgt accgcagatc gatgcggagt cctacatcct gattgactat
    31741 aactccggca aagtgctcgc cgaacagaac gcagatgtcc gccgcgatcc tgccagcctg
    31801 accaaaatga tgaccagtta cgttatcggc caggcaatga aagccggtaa atttaaagaa
    31861 actgatttag tcactatcgg caacgacgca tgggccaccg gtaacccggt gtttaaaggt
    31921 tcttcgctga tgttcctcaa accgggcatg caggttccgg tttctcagct gatccgcggt
    31981 attaacctgc aatcgggtaa cgatgcttgt gtcgccatgg ccgattttgc cgctggtagc
    32041 caggacgctt ttgttggctt gatgaacagc tacgttaacg cactggacct gaaaaatacc
    32101 cacttccaga cggtacatgg tctggatgct gatggtcagt acagctccgc gcgagatatg
    32161 gcgctgatcg gccaggcatt gatccgtgac gtaccgaatg aatactcgat ctataaagaa
    32221 aaagaattta cgtttaacgg tattcgccag ctgaaccgta acggcctgtt atgggataac
    32281 agcctgaatg tcgacggcat caaaaccgga cacactgaca aagcaggtta caaccttgtt
    32341 gcttctgcga ctgaaggcca gatgcgcttg atttctgcgg tgatgggtgg acgtactttt
    32401 aaaggccgtg aagccgaaag taaaaaactg ctaacctggg gcttccgttt ctttgaaacc
    32461 gttaacccac tgaaagtagg taaagagttc gcctctgaac cggtttggtt tggtgattct
    32521 gatcgcgctt cgttaggggt tgataaagac gtgtacctga ccattccgcg tggtcgcatg
    32581 aaagatctga aagccagcta tgtgctgaac agcagtgaat tgcatgcgcc gctgcaaaag
    32641 aatcaggtcg tcggaactat caacttccag cttgatggca aaacgatcga gcaacgcccg
    32701 ctggttgtgt tgcaagaaat cccggaaggt aacttcttcg gcaaaatcat tgattacatt
    32761 aaattaatgt tccatcactg gtttggttaa aaattgaaca cttgaaagtg taatttccgt
    32821 ccccatatac taagcatcag taaaaaaact cccgccttct ggcgggagtt gctatttaat
    32881 tacgttacgc cggagctgac atgaaaacca aacttaacga actgcttgaa ttccctactc
    32941 cttttactta caaagttatg gggcaggcgt tacctgagct ggttgatcag gtggttgaag
    33001 tggtacagcg ccatgcgcca ggtgactaca ccccaacggt aaaaccaagc agcaaaggca
    33061 actaccactc ggtatctatc actatcaacg ccactcatat cgagcaggtt gaaacactgt
    33121 atgaagaact gggcaaaatc gatattgtcc gcatggttct gtaactcgct tctccgttac
    33181 ccggctcctg gtcgggtaac tcccctctcc tcgctgtgat atacttttcc cccactttta
    33241 ctcattctcc acggagatgc cgttttgtat caggataaaa ttcttgtccg ccagctcggt
    33301 cttcagcctt acgagccaat ctcccgggct atgcatgaat tcaccgatac ccgcgatgat
    33361 agtacccttg atgaaatctg gctggtcgag cactatccgg tattcaccca aggtcaggca
    33421 ggaaaagcgg agcacatttt aatgccgggt gatattccgg tgatccagag cgatcgcggt
    33481 gggcaggtga cttatcacgg gccgggacaa caggtgatgt atgtgttgct taacctgaaa
    33541 cgccgtaaac tcggtgtgcg tgaactggtg accttgcttg agcaaacagt ggtgaatacc
    33601 ctggctgaac tgggtataga agcgcatcct cgggctgacg cgcctggtgt ctatgtcggg
    33661 gaaaagaaaa tttgctcact gggtttacga attcgacgcg gttgttcatt ccacggtctg
    33721 gcattaaacg tcaatatgga tctttcacca tttttacgta ttaatccttg tgggtatgcc
    33781 ggaatggaaa tggctaaaat atcacaatgg aaacccgaag cgacgactaa taatattgct
    33841 ccacgtttac tggaaaatat tttagcgcta ctaaacaatc cggacttcga atatattacc
    33901 gcttaattcc atacatcaat ggcccaattt acactgggtc attactcata atacctgcta
    33961 atgctttaca tctccttcgc gtttgtatat tttctttatc acttgcagaa atgcggtgcc
    34021 acgccttgct ccgtaactca ccccattgat taacctattg aaatagttga ttatggtgag
    34081 caaaagcgtt ccgttccaat tagacataag attattgtaa ttttttaaat attatttttc
    34141 catgaataaa attggagtaa gtgcgtggat agtaataatc aaattgaacc ctgtttaagc
    34201 agaaagtcgt cagaaggtaa accacaaata tttacaacct tacgaaatat tgatcttaac
    34261 cttctgacta tttttgaagc tgtatatgta cataaaggga tcgttaatgc agcgaaagtg
    34321 cttaatctga ccccctcggc aatcagtcag tctattcaga aactgcgcgt tatattccct
    34381 gacccattgt ttattcgcaa aggccagggt gtcactccta ccgcatttgc gatgcatcta
    34441 catgagtata tcagtcaggg ccttgagtcc attcttggcg cgctggatat cgaaggaagc
    34501 tatgataagc aacgaacgat aactattgcg accactccct cggtcggagc cctggtcctt
    34561 cctgtcatct atcgggcgat taaaactcac tatccgcagc ttttactgcg caacccgccc
    34621 atcagcgacg cggaaaacca actcagtcag tttcaaaccg atctcatcat cgataacatg
    34681 ttttgcacca atcgtacggt gcaacatcat gttctgttca ccgacaatat ggtgttaatt
    34741 tgccgcgagg gaaatccact actctcttta gaagatgaca gagagactat cgacaacgct
    34801 gcgcatgtac ttctgttacc ggaagggcaa aatttcagcg gtctgcggca gagagttcaa
    34861 gagatgtttc cggaccggca aatcaatttc accagctaca acattttgac aatcgctgca
    34921 ctggttgcca acagtgacat gttagcgatt attcccagcc gtttttataa cctgtttagt
    34981 cgttgctggc cgctggaaaa actgcctttt ccgtccttaa atgaggagca aatagatttc
    35041 tctatccact acaacaaatt cagcctgcgt gatcccatcc tgcacggagt aatagatgtt
    35101 atccgtaatg cattttgaaa aagtattgtt acagccagat cacagcgcag aacgataaaa
    35161 tggcacaatc caacaacaat tttacatttt cgcgaccgct ttggctgctt tcaggtccgt
    35221 ttcaatgata tactgccagt cgttaattca aaaatagttg ataattacaa caatctattg
    35281 aattgaaacg ctttccttcg taattcgcaa ctggaacacg cacgctatga gtaaacccat
    35341 tgtgatggaa cgcggtgtta aataccgcga tgccgataag atggccctta tcccggttaa
    35401 aaacgtggca acagagcgcg aagccctgct gcgcaagccg gaatggatga aaatcaagct
    35461 tccagcggac tctacacgta tccagggcat caaagccgca atgcgcaaaa atggcctgca
    35521 ttctgtctgc gaggaagcct cctgccctaa cctggcggaa tgcttcaacc acggcacagc
    35581 aacgtttatg atcctcggcg ctatttgtac ccgccgttgt ccgttctgtg atgttgccca
    35641 cggtcgcccg gtagctcctg atgccaatga accagtgaaa ctggcgcaga ccattgccga
    35701 tatggcgctg cgttatgtgg ttatcacctc cgttgaccgt gatgacctgc gcgatggcgg
    35761 tgcccaacac tttgcggatt gtattactgc cattcgggaa aaaagcccgc aaatcaaaat
    35821 tgaaactctg gtgccggatt tccgcggtcg aatggatcgt gctctggata ttctgactgc
    35881 aacgccccca gatgtgttca accataacct ggaaaacgta ccgcgtattt accggcaggt
    35941 acggcctggc gcagattaca actggtcgct gaagctgctg gaacgcttta aagaagcgca
    36001 tccggaaatc ccgaccaagt ctggtctgat ggtgggtctg ggtgaaacca atgaagaaat
    36061 tattgaggta atgcgcgacc tgcgccgtca tggtgtgacg atgttaacgc tggggcaata
    36121 tttgcagcca agccgccatc acctgccggt tcaacgttac gttagcccgg atgagttcga
    36181 cgaaatgaaa gccgaagcgc tggcgatggg ctttacccat gctgcatgcg gtccgtttgt
    36241 ccgctcttct taccacgccg atttgcaggc gaaagggatg gaagttaagt aatcctgtaa
    36301 taattactta catctgtctg ataaaaaacc cgctttttga agcgggtttt ttgtatcgaa
    36361 caagatattg agggagcgtc ctgctcgcca cgtcactctt tatgagagag cttttcagcc
    36421 tgaagatcaa cgtctgcgcc ttttttcgcc gcagcatcgt catcattcat cgccttcttg
    36481 aaccctttaa tggccgctcc aaggtctccg cccagcgtac gtaacttctt agtcccaaac
    36541 agcagaacga ccagcgccgc aactaccagc agtttggtaa tactaatctc acccatagat
    36601 accttcttga catataaccg gcaacagaac gcgccttaaa aaccagagaa tattaacggt
    36661 cattcgacgc gcgcacacaa tagcaatctg taacaaggtg aatcaagcgc cgagtaaaaa
    36721 aacatcataa taattgcggc ggcgcaaagc gacggttgtt taagatgggc agttgcgcgc
    36781 gcacctgacg cacacgttcg ggcgtcactt ccgccataat gagtgcaggc atttctgacg
    36841 ctgccgcaat ggtgacgcca aagggatcta taatccggct ttgcccgata tttttgttcc
    36901 cgcactcccc cgccgccacc atataacagg tggtatccag cgcacgagcg gcaagcaacg
    36961 ttgaccagtg atgctctttg agcggcccac gaacccaggc ggcaggaagt accaggattt
    37021 cagctccctg taaagcctgt gccagcgcca gctctggaaa gcgtaagtca taacaggtca
    37081 tcagaccgac cttcatccct tccacctcca gtaacggagc tatttcatta ccagcatcaa
    37141 catggcgtga ttcctgaatg gcaaatgcat catagagatg cagtttggca taacgggcga
    37201 cgatgtttcc tgcctgaagt gccaccagca tattccatgc gcgccccggc gttgaaggaa
    37261 gatgaatcgt cagaattgtc gtcatcatgt tacgtttact ttctcgccgt aaacgtccga
    37321 ggaattcgcc ttccagcagc tgtgctgatt taaccgatag atctgcatca tgatcatcgc
    37381 gcgccagcaa tgcttccggc aggacaaaca gcgatacgtc gttttccgcc gcctgcgcca
    37441 tcaacgaggc acaaatctca gcgttctttt cccacacaga tgtaacagca aactgtcctg
    37501 cagcaaccag cattgccacc tcctgtcaga gtcgtaaaat tcgccaacga cgattattcg
    37561 gcgttacact tatcactcat acaaatcaaa tagcaggatt ttgcagtgtt acaacttctt
    37621 ttagcagttt ttattggcgg tggtacggga agcgtagcga gatggctgtt aagtatgcga
    37681 tttaacccgc tgcatcaggc gattccgttg gggacgctgg cagcaaacct gattggggca
    37741 ttcatcatag gaatgggatt cgcctggttc agcaggatga cgaacattga tccagtgtgg
    37801 aaagtattaa tcaccaccgg attttgtggc ggtctaacaa ccttctcaac attttcggca
    37861 gaagtggtgt ttttgttaca agagggccgc tttggctggg cattactgaa cgttttcgtc
    37921 aaccttctgg ggtcttttgc catgaccgca ctggcattct ggctgttttc ggcctcaacc
    37981 gcacactaaa gaccaaaaaa aacccgctga ttaagcgggt tttgaattct tgctgacgta
    38041 tcttacagag cgattacgtt tgcagcagaa gggcctttgg caccgttagt gatttcgaac
    38101 tctacgcgct gaccttcagc aagagtttta aaaccattag tctggattgc agagaagtgt
    38161 acgaacacgt ctttgctgcc gtcttccgga gtaatgaaac cgaatccttt ggactcatta
    38221 aaccacttaa cgttaccttt aatcttagac atcaaaatta cctttacatg aaaaatagac
    38281 acaaatgctg tgtcggttat cagtacacca attgtggtac gctttgtcca gtgcaacttt
    38341 actaaaaagt gataaaagtc gcgttatttt tgatgttgct tattttagtt attgatttta
    38401 aagaagttac taaaacttca tttgtctcaa aactgaaagc gcatccaggc aaagtacaca
    38461 ttgccattgt tgtaggtacc cggaatgtag gtcatctgaa aagtcactgg gccataaccc
    38521 acggaggcca atggcagtag aaccgggaga gggatgtaat tccagttatc gcgtgccgtt
    38581 acgccagcgg tgaatcccag acctaaatga aaattttcat ccgccagcgg tcgccaggta
    38641 ctttcccatc cgtatccggc aatcggttcc catttgttcc acgagtcctt aaatgccatg
    38701 gcatacaggc catgccagtt tcctttttca tcccaacgcg acaggccaaa accgccaccc
    38761 cacggtcgct cgttatagcg atcggttttt tctttgtcgt aagcgaaacg tgcatgccag
    38821 gtgatggcag gaatatataa atcataatgt tcaggctgtt gccaggtttg tgcaatattt
    38881 tctctaaacg ttgtcatcct ctcatctgcg ttagcaaaaa ctttaccaac gctgattaac
    38941 tgaataaaaa caaaggaaaa gatagcgaca tatttactca cgttcatttg tgaccataaa
    39001 acatttatca aaaatctact actagcatag caaagctaca attaacataa ccttaataga
    39061 cccaacataa agaataatat gaatagctgc tctatttatt tccgcttgtt caagttaaat
    39121 atttccgcgc atcgattaaa gatgatgcgt cgttttctgc gatgggaata gtcaaaaaag
    39181 aaaaaccaag tcttttttga tgacaaaatg caatcaagga aaaattattt tattttttaa
    39241 cgagatacaa caatcatctt aacgaagtat ataatatata tcgtcaaccc atgctcatat
    39301 tagattattt tttgagcgca cagccgcagc taacaccatt gcaattaaca aatttgcatc
    39361 aatccgccat caatttacac acattattat gtgataattg ccaaccgcta aatatgcgtt
    39421 ttgttatcta tgtataaaaa cagcaacttc aatgtcttaa tggcagtttt tcttgatttt
    39481 aatcagcatt catcgccaat ttattgggca tattttttcc ttaagcttta ggaatttttt
    39541 attatttact ttgggcctgg agacaggaaa aattatgctg acattcattg aactccttat
    39601 tggggttgtg gttattgtgg gtgtagctcg ctacatcatt aaagggtatt ccgccactgg
    39661 tgtgttattt gtcggtggcc tgttattgct gattatcagt gccattatgg ggcacaatgt
    39721 gttaccgtcc agccaggctt caacaggcta cagcgccacg gatatcgttg aatacgttaa
    39781 aatattacta atgagccgcg gcggcgacct cggcatgatg attatgatgc tgtgtggctt
    39841 tgccgcttac atgacccata tcggcgcgaa tgatatggtg gtcaagctgg cgtcaaaacc
    39901 attgcagtat attaactccc cttacctgct gatgattgcc gcctattttg ttgcttgtct
    39961 gatgtcactg gccgtctctt ccgcaaccgg tctgggtgtt ttgctgatgg caaccctgtt
    40021 tccggtgatg gtaaacgttg gtatcagtcg tggcgcagca gctgccattt gtgcctcccc
    40081 ggcggcgatt attctcgcac cgacttcagg ggatgtggtg ctggcggcgc aggcttccga
    40141 aatgtcgctg attgacttcg ccttcaaaac gacgctgcct atctcaattg ctgcaattat
    40201 cggcatggcg atcgcccact tcttctggca acgttatctg gataaaaaag agcacatctc
    40261 tcatgaaatg ttagatgtca gtgaaatcac caccactgcc cccgcgtttt atgccatttt
    40321 gccgttcacg ccgatcatcg gtgtactgat ttttgacggt aaatggggtc cgcaattaca
    40381 catcatcact attctggtga tttgtatgct gattgcctcc attctggagt tcctccgcag
    40441 ctttaatacc cagaaagttt tctctggtct ggaagtggct tatcgcggga tggcagatgc
    40501 gtttgctaac gtggtgatgc tgctggttgc cgctggggta ttcgctcagg ggcttagcac
    40561 catcggcttt attcaaagtc tgatttctat cgctacctcg tttggttcgg cgagtatcat
    40621 cctgatgctg gtattggtga ttctgaccat gctggcggca gtcacgaccg gttcaggcaa
    40681 tgcgccgttt tatgcgtttg ttgagatgat cccgaaactg gcgcactctt ccggcattaa
    40741 cccggcgtat ttgactatcc cgatgctgca ggcgtcaaac ctgggtcgta ccctatcacc
    40801 cgtttctggc gtagtcgttg cggttgccgg gatggcgaaa atctcaccat ttgaagtcgt
    40861 aaaacgcacc tcggtgccgg tgcttgttgg gctagtgatt gttatcgttg ctacagagct
    40921 gatggtgcca ggaacggcag cagcggtcac aggcaagtaa atagtaatgc cggaggcaag
    40981 tttcctccgg ctttatcatg tcacccactg tggtatatgc gttgtggtct gccaactttg
    41041 ccgtgaacaa tttcagcaat aatcagatgg cggctggcgc aatattcaag ataacgcctg
    41101 gcagtggtgc ggctgatggt cagtgcctgc gccaccgttt ctgccgtatg ttgcacacca
    41161 ggctctttaa acagttttcg caccgcgttt agcgtcaagg ggtcaatgcc ggtcggcagt
    41221 tcgtctttgg gttcaccgcg agcataggca ttaaacatct catcaatttg cttctggctg
    41281 gcgctatcaa tactttccag catatgttta cgctggcgga aacgggttag cgtttgcccc
    41341 agtgtattga ttcacttgaa gtacgaaaaa accgggagaa cattggatta ttcgggatct
    41401 gatgggatta gatttggtgg ggcttgcaag cctgtagtac aaattttagg cgttaaccaa
    41461 tgaaacgcga aagatagtaa aaaattgctt ttgtttcatt gaaaaaacga aaaacaaaaa
    41521 cactataaat
//
LOCUS       DBKEKI010000022        40563 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.022, whole genome shotgun sequence.
ACCESSION   DBKEKI010000022 DBKEKI010000000
VERSION     DBKEKI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.022"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..956)
                     /locus_tag="ACUQPX_001128"
     CDS             complement(<1..956)
                     /locus_tag="ACUQPX_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HHV9055896.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGS"
     gene            1203..1829
                     /gene="gstB"
                     /locus_tag="ACUQPX_001129"
     CDS             1203..1829
                     /gene="gstB"
                     /locus_tag="ACUQPX_001129"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HHV9055897.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNSNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1826..2941)
                     /gene="yliI"
                     /locus_tag="ACUQPX_001130"
     CDS             complement(1826..2941)
                     /gene="yliI"
                     /locus_tag="ACUQPX_001130"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HHV9055898.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(3052..3435)
                     /gene="bssR"
                     /locus_tag="ACUQPX_001131"
     CDS             complement(3052..3435)
                     /gene="bssR"
                     /locus_tag="ACUQPX_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HHV9055899.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3648..4973
                     /gene="rimO"
                     /locus_tag="ACUQPX_001132"
     CDS             3648..4973
                     /gene="rimO"
                     /locus_tag="ACUQPX_001132"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HHV9055900.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5020..6348)
                     /locus_tag="ACUQPX_001133"
     CDS             complement(5020..6348)
                     /locus_tag="ACUQPX_001133"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HHV9055901.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6356..8704)
                     /locus_tag="ACUQPX_001134"
     CDS             complement(6356..8704)
                     /locus_tag="ACUQPX_001134"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HHV9055902.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEVIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8882..9793)
                     /gene="gsiD"
                     /locus_tag="ACUQPX_001135"
     CDS             complement(8882..9793)
                     /gene="gsiD"
                     /locus_tag="ACUQPX_001135"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HHV9055903.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLTGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9796..10716)
                     /gene="gsiC"
                     /locus_tag="ACUQPX_001136"
     CDS             complement(9796..10716)
                     /gene="gsiC"
                     /locus_tag="ACUQPX_001136"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HHV9055904.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSIVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10734..12273)
                     /gene="gsiB"
                     /locus_tag="ACUQPX_001137"
                     /pseudo
     CDS             complement(10734..12273)
                     /gene="gsiB"
                     /locus_tag="ACUQPX_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
     gene            complement(12293..14164)
                     /gene="gsiA"
                     /locus_tag="ACUQPX_001138"
     CDS             complement(12293..14164)
                     /gene="gsiA"
                     /locus_tag="ACUQPX_001138"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HHV9055905.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEYAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCEVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14151..15116)
                     /gene="iaaA"
                     /locus_tag="ACUQPX_001139"
     CDS             complement(14151..15116)
                     /gene="iaaA"
                     /locus_tag="ACUQPX_001139"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HHV9055906.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALSFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15320..16555
                     /gene="moeA"
                     /locus_tag="ACUQPX_001140"
     CDS             15320..16555
                     /gene="moeA"
                     /locus_tag="ACUQPX_001140"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HHV9055907.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16555..17304
                     /gene="moeB"
                     /locus_tag="ACUQPX_001141"
     CDS             16555..17304
                     /gene="moeB"
                     /locus_tag="ACUQPX_001141"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HHV9055908.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17483..18145)
                     /gene="fsa"
                     /locus_tag="ACUQPX_001142"
     CDS             complement(17483..18145)
                     /gene="fsa"
                     /locus_tag="ACUQPX_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HHV9055909.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            18276..19175
                     /locus_tag="ACUQPX_001143"
     CDS             18276..19175
                     /locus_tag="ACUQPX_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HHV9055910.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHRKKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLPAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19181..21613
                     /locus_tag="ACUQPX_001144"
     CDS             19181..21613
                     /locus_tag="ACUQPX_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HHV9055911.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21759..22574
                     /gene="ybiV"
                     /locus_tag="ACUQPX_001145"
     CDS             21759..22574
                     /gene="ybiV"
                     /locus_tag="ACUQPX_001145"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HHV9055912.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22726..23991
                     /locus_tag="ACUQPX_001146"
     CDS             22726..23991
                     /locus_tag="ACUQPX_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HHV9055913.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24232..25824)
                     /locus_tag="ACUQPX_001147"
     CDS             complement(24232..25824)
                     /locus_tag="ACUQPX_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HHV9055914.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26043..26963
                     /gene="ldtB"
                     /locus_tag="ACUQPX_001148"
     CDS             26043..26963
                     /gene="ldtB"
                     /locus_tag="ACUQPX_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HHV9055915.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27021..28142)
                     /locus_tag="ACUQPX_001149"
     CDS             complement(27021..28142)
                     /locus_tag="ACUQPX_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HHV9055916.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAVGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLLAWAVNVGGFGLLPGSLANLI
                     ALRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28139..28606)
                     /gene="mntR"
                     /locus_tag="ACUQPX_001150"
     CDS             complement(28139..28606)
                     /gene="mntR"
                     /locus_tag="ACUQPX_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HHV9055917.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28792..28920
                     /gene="mntS"
                     /locus_tag="ACUQPX_001151"
     CDS             28792..28920
                     /gene="mntS"
                     /locus_tag="ACUQPX_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HHV9055918.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29192..30778
                     /gene="opgE"
                     /locus_tag="ACUQPX_001152"
     CDS             29192..30778
                     /gene="opgE"
                     /locus_tag="ACUQPX_001152"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HHV9055919.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETSFVQSKETSCYL
                     YTMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQV
                     PFMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITN
                     FQLQKVDYNHLGTDIFDPKP"
     gene            complement(30827..31342)
                     /gene="ompX"
                     /locus_tag="ACUQPX_001153"
     CDS             complement(30827..31342)
                     /gene="ompX"
                     /locus_tag="ACUQPX_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HHV9055920.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31395..31460)
                     /locus_tag="ACUQPX_001154"
     CDS             complement(31395..31460)
                     /locus_tag="ACUQPX_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9055921.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31695..32582
                     /gene="rhtA"
                     /locus_tag="ACUQPX_001155"
     CDS             31695..32582
                     /gene="rhtA"
                     /locus_tag="ACUQPX_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HHV9055922.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32881..33384
                     /gene="dps"
                     /locus_tag="ACUQPX_001156"
                     /gene_synonym="pexB"
     CDS             32881..33384
                     /gene="dps"
                     /locus_tag="ACUQPX_001156"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HHV9055923.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLDGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33788..34534
                     /gene="glnH"
                     /locus_tag="ACUQPX_001157"
     CDS             33788..34534
                     /gene="glnH"
                     /locus_tag="ACUQPX_001157"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HHV9055924.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            34673..35332
                     /gene="glnP"
                     /locus_tag="ACUQPX_001158"
     CDS             34673..35332
                     /gene="glnP"
                     /locus_tag="ACUQPX_001158"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HHV9055925.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35329..36051
                     /gene="glnQ"
                     /locus_tag="ACUQPX_001159"
     CDS             35329..36051
                     /gene="glnQ"
                     /locus_tag="ACUQPX_001159"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HHV9055926.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVIQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36312..38537
                     /gene="ybiO"
                     /locus_tag="ACUQPX_001160"
     CDS             36312..38537
                     /gene="ybiO"
                     /locus_tag="ACUQPX_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HHV9055927.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVTELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAPPAEPLPPGEPT
                     L"
     gene            complement(38534..39460)
                     /gene="rlmF"
                     /locus_tag="ACUQPX_001161"
     CDS             complement(38534..39460)
                     /gene="rlmF"
                     /locus_tag="ACUQPX_001161"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HHV9055928.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39736..39996
                     /gene="mcbA"
                     /locus_tag="ACUQPX_001162"
     CDS             39736..39996
                     /gene="mcbA"
                     /locus_tag="ACUQPX_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HHV9055929.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            40261..>40563
                     /locus_tag="ACUQPX_001163"
     CDS             40261..>40563
                     /locus_tag="ACUQPX_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HHV9055930.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT        10083 a        10733 c         9879 g         9868 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgctcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atcggccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcgggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaaatac gttatatcgc cgtatgataa gttcggtgat
     1021 taataacgca cacacaaact taaagaaacc gtaagttggt aaagttttta aagtttatgc
     1081 aatatcctgc cgcttcgctg cttctggtgt catttttttg ccagctgttg cttaaaagtt
     1141 aactttactt caccatcgga gtcctgttca gtctgtggct gcctgtcgga aaaggggtga
     1201 gtatgattac gctgtggggt cggaataatt caactaacgt aaaaaaagta ttgctgacgc
     1261 tcgaagaact ggaactacct tatgagcaaa ttctcgcggg ccgtgagttt gggataaatc
     1321 acgatgctga ttttctggcg atgaactcta acgggctggt gccgttgttg cgtgacgacg
     1381 aaagtgatct cattctttgg gaatcaaacg ccattgtccg ctatctggcg gcacagtacg
     1441 ggcaaaaacg cctgtggatc gactcaccgg cacgtcgtgc ggaagcggaa aaatggatgg
     1501 actgggcaaa ccagacgctc agtaatgctc atcgcgggat cctgatggga ttagtcagaa
     1561 caccaccgga agagcgcgat caggccgcca ttgatgccag ttgcaaagag tgcgacgccc
     1621 tgtttgccct gctcgatgcg gaactggcaa aagtaaaatg gttctctggc gacgagtttg
     1681 gtgtgggcga tatcgctatc gcaccgttca tctacaattt gtttaacgtt ggcctgacct
     1741 ggacaccgcg tccaaatctg caacgctggt atcagcaact cactgaacgc cccgcagtgc
     1801 gcaaagtggt gatgattccc gttagctaat tgcgtgggct aactttaagt aattccccac
     1861 tggactcgtc ggtgagaacg tataaataac cgtcgggtcc agtgcgaaca tcacgaattc
     1921 gctgccctct gtccgttaaa atacggccat cttctgtcac tttgtcgccg ttgacgctca
     1981 tcacaatgac atctttatct ttcagcgcgc caataaataa tttttgctgc cactggggga
     2041 atttatcgct gttatagaag gccatgccgc tcacagcggg cgaatctttc cagtaaaaaa
     2101 caggttgctc ggtcccggcg acgatctccc ctttcgcttc cggtatctta aagcctgaat
     2161 agttgattcc ccaggttgcc agcggccagc cgtagttttt gcctttttgc gggatattaa
     2221 tttcatcacc accgcgcggg ccatgttcat tcagccacag tgcattactc cacggattca
     2281 tcgccattcc ttgcggatta cgaatgccat aagaccagat ctcggcgcgc gcaccggatt
     2341 cctttataaa aggattatca tccgggattt cgccctggtc ggtcagacgc accagtttgc
     2401 cctgtaattt atccagatcc tgcgccgtcg ggcgctgatt gttttcgccc agagcaataa
     2461 aaagataacc tttaccgtcg aataccagcc gcccgccaaa atggttgccg gtagacagtt
     2521 ttggcatctg gcggaagacg gtgcggaagt cggtcacttt tgagagatca tcacttaagc
     2581 ggccataacc cacagcagtt ccggctttgc catcatcgcc aacttcggaa taacttaacc
     2641 agatgcggcg agactgagca aaatcaggcg ctaaaaccac gtccagcagg ccgccctgcc
     2701 cgtgcgccca aacgtccgga actccggaaa gcggcgcaga taatcctttt cctgcttgcc
     2761 agtgacgcaa ctcgccgccg cgcagagtga ttaacatacc gtgattatcg ggtaaaaagg
     2821 ccagtgccca gggatggtcg agtttgtctt gcagtacttc gacatttacc gttgcaggag
     2881 ccgcccagag agcggaagaa agacaaataa ggggcacaag gaaaaaggat tgtcgatgca
     2941 taacgcctcc ggattgactc tggcttaaag cgtagtcagt ggaggagata acaaattcat
     3001 ttttacaaaa acttaaacat gaagggggag acgctttctc ccccttagtt ttcaggcctt
     3061 ctcaagcatg gcgtgcttct gcaggctctg gatactcagc gttaagctca tcagacaatt
     3121 ttcaagctta tcggcgttga cggtaataac agtcgggcaa tcatggtgcc cactcatcaa
     3181 acatactgcg gctgtcgcta atgcttcttc agcatgatga agagcactcc actcttcctg
     3241 atccagatga agattcaacc gcagcgattt atcgtgcagt tcgcgattca gtttaaaaaa
     3301 gttatctcgt agatgattgc tttcgctgac ggacatgtat ccttttgcct tcctcagttg
     3361 cacgtatgca gcgaggtcaa cgcgtgcgtc gatcaaccgg ttcagcagat cgattcgctg
     3421 tctgtcaacg aacatacttc gatcctcctc tttccagcct tgttgcaatt aaatagtatg
     3481 gctatttttt gagcaaaatg ctgtccagga tcaattcttc acgccgaatt aacaaaattt
     3541 acatttcctt cgcctccgcc agcgaatgag attgcgatac aaattttatc cagaactctg
     3601 tagaatccct gccctgacct ttcctgtaac tgaatatttt ttaagctatg agcaaagtaa
     3661 ctccccagcc gaaaatcggc tttgtttccc ttggctgtcc gaaaaacctt gtcgattcag
     3721 agcgtattct caccgaactc cgcactgaag gttatgacgt ggtaccgagc tatgacgatg
     3781 cggacatggt gatcgtcaac acctgcggct ttattgacag cgcggtacaa gaatcactgg
     3841 aagccattgg tgaagcgttg aatgaaaacg gcaaggtaat tgtgaccggt tgtctggggg
     3901 caaaagaaga tcagatccgc gaagtccacc cgaaagtgct ggaaatcacc gggcctcata
     3961 gctatgagca ggttctggag cacgttcatc actacgtgcc aaaaccgaaa cacaacccat
     4021 tcctgagcct ggtgccagaa caaggtgtga aactgacgcc gcgtcattat gcctatctga
     4081 aaatttcgga aggctgtaat caccgctgca ccttctgcat tattccgtct atgcgcggcg
     4141 acctggtgag ccgtccgatt ggcgaagtgt taagtgaagc gaaacgtctg gtagatgcgg
     4201 gcgttaaaga gattctggtg atctcgcagg atacttccgc ctatggcgtt gatgttaaac
     4261 atcgtactgg cttccacaac ggcgagccgg taaaaaccag catggtcagc ctgtgcgaac
     4321 agttatcgaa actggggatc tggacacgtc tgcactacgt ttacccttat ccgcatgtgg
     4381 acgacgtcat cccactgatg gcagaaggca aaatcctgcc gtatctggac attccgttgc
     4441 agcacgccag cccgcgcatt ctcaaactga tgaagcgtcc gggttctgta gatcgccaac
     4501 tggcgcgcat caaacagtgg cgcgaaatct gcccggaact gaccctacgc tcaaccttta
     4561 ttgtcggctt ccctggcgag acggaagaag atttccagat gctactcgac ttcctgaaag
     4621 aagcacgtct ggatcgcgtt ggctgcttta aatacagccc ggttgaaggt gcagacgcca
     4681 atgccctgcc tgaccaggtt ccggaagaag tgaaagaaga acgctggaac cgtttcatgc
     4741 agctgcagca gcagatttcc gccgagcgcc tgcaagagaa agtgggccgt gaaattctgg
     4801 tgattatcga cgaagtggac gaagaaggcg cgattggtcg cagcatggca gatgcaccgg
     4861 aaatcgacgg cgcggtttat ctcaatggtg aaaccaacgt taagccgggt gatatcctgc
     4921 gtgtgaaagt cgagcacgcc gatgagtacg atttgtgggg tagccgggtt taattaattc
     4981 aggccgggca aaatcatttg tccggcctgc gaataatgat tactgccagc taatctgtgc
     5041 cctgccgttt ttcttactct tatacagccg ctcatccgcc agcattttca agcgttcgac
     5101 ggtagtacat tcatttgaat gcgctacacc gatactggtg cttaaattaa tgcttcccac
     5161 ctggaaatga acgatttctt ttagtatcga tagcaactct tccagcgtgc cgccagtata
     5221 aatcaccgca aattcgtcac ctccgaagcg gtagacttta cctttatcta ccgggatctg
     5281 tgatttaagg attttcacca ctttcatcaa cacttcatcg cccagcaaat gaccgaaggt
     5341 gtcgttaatg tttttgaatt tatcgacgtc aaacagacac aaataaccgc ctttttccgg
     5401 cagcgctttt aaatcttcgt caaaggattt tctgcccatt gctttggtca ggtcgtccag
     5461 gaacgccgag ttatacatac tgtagagttt tttgacatcg gcagccgaaa agaaaagccc
     5521 gccatccagt acgcgttcaa aatggttatt aatgatggct tctttctcca caataccacg
     5581 attaatcact ctgcgtacca gatagaggca actggcaccc gatatcactg ccaccaatgc
     5641 ggcaaagaaa ataatcaatg tacaaacccg caaataaaca tcttttttat acgcaaatac
     5701 gtacaatccg gtcggcaaat aggcgaagtg ctccatcgct tccgaaacgg tgaagatggc
     5761 gtttttagca ttatattcat caccggtcac cggtttaccg ttcttaaata gagagaacat
     5821 caggttctcg ctgtccatcg ccagcgattt tgacagtaaa gaaaaacgct ccagaccaag
     5881 caaaaatatc agactacgat tgtgagttac cggatagagg taatagacga aggttttacc
     5941 gtcaaaagag ataaaccctg tatttttgac atgtcgatac ttcacaacct gcataatatc
     6001 tgcacaataa tttttctcga actcctttac ctgaatacat tcgtcactgg aaatcagata
     6061 aggaccgaca tcgtacaaat aagaggggga aaccgagtcg gcgtaagctg tgcgcaaacc
     6121 aaccaacagc tgttcctccg tgggatttag cgtgttgata agatcaattg ccacgctacg
     6181 ccgatattca gcaatatttg ccaccgcaaa agcagtatca ataacggact gtttcaggct
     6241 atacacccgt tcctttacca tttgagtgta gatcccgcag gcaactgctg aaatcatgat
     6301 aaaaatcagc agactgactg taagtacgaa cttattgatt ctggacatac gtaaactact
     6361 cttttactaa ttttccactt ttatcccagg cggagaatgg catgggtttc tggtacagat
     6421 acccctgagc ctgaacacat cctaatttaa ttaattcgcg cgcaacgtct tcgctctcta
     6481 cgccttccgc aatcaccggg atccggttat agcgtgccag atcgaaaata atgcgcacaa
     6541 tttttcccgc agtaccgaaa atatcgccgg taaattgcga gtccaccttt atgctgtctg
     6601 gattaatttc ataaaaacgt ttcaggtttg acaaaccggt gccaaagtca tcaatcgata
     6661 tccgtacccc ttgtttacgc agttgttcaa tattgagata aaaatgttca ttaattcgct
     6721 cgatcacggt ttcagtgatc tcaacacaga ggctgttcgc cttcaggcca ttttctttca
     6781 tgatggtggt aaaacgctca tgaaagtgcg gttcgtttaa ctgccagggc gaaacattaa
     6841 tatgcagtaa gaaatcatcc ttcagcgacg cacgttgact aaaatgtctg aattcaccca
     6901 ttgccgtttt aataatctga taacccagct cattgataaa cccgatatct tcagcaatgg
     6961 taatgaactt cagcggtgaa atgatccccc gctccgcaga aacccaacgg cacaacgcct
     7021 ccgcgctcag aatggcgcgg gtgttaatat caacgatcgg ttgatagacg aggaatagtt
     7081 cgccaatttc cattgcatgg cgaatattac gcgcaatcca gatattctct tctatttcgt
     7141 tgagtttcgc gtcatcacag aaaataaact cggagaacat atggtcacgg atactggaaa
     7201 tcgccagttt cagtttttct gcgtaactcc aggcgttttc cccctgcaaa ggttcacaaa
     7261 tggcagactt atggatgcgc agattgcggt agccaaagct gctgtcaaaa tcgtttacca
     7321 tcgctatttt acgggtaaat gtctgtacat tttcccgaca cagaagtaaa tagagatcgg
     7381 cattgaatgt cacgatatcg cagcattgcg catatttctc ggcaattcgc tgacacaatt
     7441 tcctgacgat tttattgcgt tcatcaacac caaacaccgc ttccacggct tccagaccac
     7501 acagtttgat gatccccaca gccagatact gattattgtt ggactcaata atcgccatat
     7561 ttttaataaa accgctttcc tgatcgtaag agatcagtga gagcaattta tttgaccgtt
     7621 caatttcggc atctttcatt tccgcgatcg aatttttgat gctgataatc tctttcgcat
     7681 cgccttcgcg aattttcaga gcctcgatct gatccgttga gtcgggcaaa aacgccagcg
     7741 tattaaccag ctcggtcatt tcagaaaatt gccgacggaa cacacgagtg gtcatgaaat
     7801 ataccaccag caacacgata ataatgaacg caaacggtag ataaaaaagt ttatctgatg
     7861 cgttcaataa attaccgcca ggataataaa aatagatttc gaacaatgca ttatttggca
     7921 ctgctgatgt ctttgcaata tactccgtat cgacagaatc aataatatta taattacctt
     7981 cgctattata aataatcagt ttttcacgag cgagttgatt cttggcaatg acctcatgac
     8041 cttgtttcaa aaatagaact tcatcattag cgctgatttg atcaagatac tcgttcagat
     8101 accgccagtt taaatcgaca tgaattttat ctaccgctaa caaatcgtga tctttgccgt
     8161 tgatacgtag cgtaaaagag atacattttt tacccggacg atcaaagcaa ttgtatgccg
     8221 gattccagct gttgttagag tcatctgaag ccaccgacca cggtcgtttc gatgggttaa
     8281 aatcggcaaa aataacccgc cctgactcgt cgacaagcgg tccatttact tgttcctgat
     8341 gataaacaac gattttatca ttcgccctcc gcgaaaagag ataatattta ttatcatcaa
     8401 gtaccagtcc caccgagtct aaaaatggca tcatttccat tttcagaatc acggcgcgtt
     8461 gaatctcctc tgtatttttt aataaataca gtgagtcctt taatgtcagg gccgaacgtt
     8521 caatatcttt aaagaaatag ccaacgtttt tctgaataag ttcaaagcgg ctatcggcac
     8581 gttttaccgc gcgttccgat accagttgat agttaattat gaaaaaaaga ccaataaatg
     8641 acagtgctgc caataaaaat aaaattatca gccttatctt tatcttttcg tataaactca
     8701 gcatgttaat acatttaaca aaataactat ctgaacgctg tgccgctgca aaaatagtcc
     8761 cttgctgcgg ctttttaacc aaattacccg attctacaaa atcagcagaa agttacaaca
     8821 cttgcttagg gcaataattt cctgtgaggt gattaccctt tcaagcaata ttcaaacgta
     8881 actatccttt aattttcgga tccagcgcat cgcgtaaacc atcgcccaac aaattgaacg
     8941 ccagtacggt cagaaaaata gccagggccg gaaaaacagc gacatgcggc gcgataacca
     9001 tatccgctcg tgcctcattg agcattgctc cccactctgg tgtcggcggc tgcgcaccga
     9061 ggccgagaaa tgacaggctg gcggcagaga taatcgaggt gccaatgcgc atggtgaaaa
     9121 acaccacgat agaagagacg gtcccaggca ggatatgacg caataaaatg gtcatatcgc
     9181 tggcaccaat actgcgtgct gactcaataa aggtttgctg tttcaacacc agcgtgttgc
     9241 cgcgcaccag gcgggcaaac gcggggatgg aaaaaatggc gactgcgata atcacgttag
     9301 caatgccgct tcccaacacc gcaacaacag cgatcgccag taaaataccc gggaaggcaa
     9361 acagcacatc gcaaatgcgc atgatcagcc gatcccacca gccttcataa tatccagtca
     9421 acaagcccag caacgtccct atcgccacac cgataaacac ggcaaacacg cccgccgcca
     9481 gcgagatttg cgcaccaacc aggacacggc tgaaaatgtc gcgccccagt gaatcgacgc
     9541 caaaccagtg ctgcaaagaa ggtccgttat tcagattgtc ataatcaaaa taattttcgg
     9601 cgtcataggg agcgatccag cgtgcaaaaa tggccaccac aatcaataaa ataacgaata
     9661 atgcggcggt catcgccata tgctgacggc gaaatcgtcg ccagaattca tgccacggtg
     9721 tacgtacctg gtcaggtttg accagtggca tggcgtttaa caccgcctga cgtcgccagt
     9781 taaatagtcg catccttact tgtacctgat agccgggtta atggcggcgt aaagcacatc
     9841 caccactaag ttgataagaa taaattccag cgagaaaagc agaatttccg cctgaatcac
     9901 cgggtaatcg cgcatttcta cagagtcaac gagtaagcgt ccaagtccgg gccagttgaa
     9961 aactttctcc accacgatag aaccaccgag caaaaagcca aactgtaagc ccatcatggt
    10021 cactaccggg atcatcgcgt tgcgtagccc gtgtttgagg acaacccagg tttcgctcac
    10081 ccctttcgcc ctcgcggtac gcatataatc ttcgcttaaa acgtcgacaa atgacgcacg
    10141 ggtaaagcgc gccatcacgg cggccactgc cgcgccgagc gtcagggagg gtaaaatgta
    10201 gtgctgccaa ctgtctgctc ccacagtagg cagccagccc agttcaacgg agaatacctg
    10261 aattaaaagc atccccagcg caaatgccgg aaacgagatc cccgacaccg caatggtcat
    10321 actcaatcga tccggccaac gattacgcca gacggcggcg ataattcccg ccgccatacc
    10381 aaatatcacc gcccagacta tactggttat ggtcagccac agcgttggca taaagcggct
    10441 ggcaatctca tcggcaaccg gacgacgcga caccatcgac aggccaaaat ccccctgcac
    10501 agcattgctg atatagtgcc agaactggtg atacagcggc tgatccaacc ccagctgctg
    10561 acgcaccagt tctataacct gcgcatcagc ttcgggcccg gcaatcaatc gcgccggatc
    10621 gccgggcagc atgtggacaa ataaaaacac cagcaccgag acgataaaca gcgtcggaat
    10681 caaccccagt aatcgtttga taacgtaatt aagcattcca ctccctgcgt tgcttattgc
    10741 aaatccgcgt cttcaaagct gaagccggtg tctggcatga tccaaaaacc ggtcaggttt
    10801 ttactgtgtg ccgacaccag tttttctacc accagcggga tccacggcga ttcttgccag
    10861 atgatatcct gcgccgcctt atataagcgg gtcttttccg ccggatcatt cgttttcagt
    10921 gcctgagcca ggaagtcatc cacctgttta ttgctgtaaa acgcggtatt aaacagcgtc
    10981 ggtggccagt tttgcgaggc aaacagcggc gatagtgccc agtcggcttc gccggttgaa
    11041 gccgaccagc cagtgtagaa catccgcacg ccgctctctt tttgcccttt accttcaact
    11101 tctgccgccc gctgtccggc atccatcgcg gtcacctggg ctttaatccc gacctgcgct
    11161 aactgctgct gggtaaattg cagcactttc tgcgcggtgc tgtggttatg tgacgaccac
    11221 agcgtggtac tgaaaccgtt gggatatccc gcctctttca gtaattcgcg cgctttcact
    11281 ggatcgtaag gccacggttt ataactttgc gcgtaggcga tacttggcgg taccacacca
    11341 gtagctggcg ttgcatagcc cgcaaaggca actttcacca gcgccggacg gttaatggcg
    11401 taattcagcg cctcacggac cttcgggtta tcgaacggct tttgcgtcac gttcatactg
    11461 atataacgct gcataattga cggactggcc atcaactcga tatttttgtt tttctccagc
    11521 agtgtggcct gctcgtaggg aatggggaaa gcaaactgcg cttcaccggt ttgcagcatt
    11581 gccgcgcggg tgttgttatc cgccaccgga cgccaggtta tgctgtccag tttgggcaat
    11641 cctggctgcc agtaacccgc gaattttttc accttcacaa aatcggtctg attccaggta
    11701 tccagttcat actggtccgg ttcccaccgg atgaaaacca atctccttgc catatttttc
    11761 cagcgctgtc ggtgaaatca tcgcggtcgc cggatgggca agaatattaa taaacgctga
    11821 gaacggctgt ttgagggtaa tctttaccgt tgtcggatcg atcgcctccg ttttggcaat
    11881 attcttatac aggttatagc gtttaagatg attcgccgga tcgctggccc ggtccagatt
    11941 cgcttttacc gccactgcgt tgaaatcggt gccatcctgg aatttaattc cttcccgcaa
    12001 tttcacggtg taagtaatgc cgtcatcgga aacggtataa ctctccgcca gcacgttttt
    12061 cagtttcatc tctttatcca gaccgaacag cccctggtaa aacgatttcg ctacggcctg
    12121 agataacgtg tcattcgcgt catacggatc gagcgtggtg aaattcgatc ctaccgccac
    12181 caccacatct ttggcagcga atgcacaaga tgccatcaac gctgtcgcaa tgcccagcgc
    12241 cactaaccca ctacggtgta cagctcttgc cattttattc tccgcctgaa tgttatctac
    12301 gcatgaatgc gtattctgat tgtggttgtg cgacgtaatg ccccggcccg acgcattgca
    12361 acgagacggc tgccacctct tcgccacgca gatgaatatt gctgggaaga tcgtccgaca
    12421 gcagtacacg ctgcggtcgt tgtcgggacg gttcagcgac cggaactgcc gccagtaatt
    12481 tacgcgtata aggatgctgc gggttttcga agacctcgca ccgtgaacca atttcaacaa
    12541 tttgcccgag atacatcacc gccacacgat gactaatccg ctctaccaca gccatatcgt
    12601 gggagataaa cagatacgca atgccgaaat cacgctggag atcgagcaac aagttgataa
    12661 tctgcccgcg aatagaaaca tccagcgccg aaacggcttc gtcggcaatg atcacttttg
    12721 gattcaatgc caacgcgcga gcaatgcaga tgcgctggcg ctgaccgccg gaaaactcat
    12781 gcgggtaacg ccaggcatat tcaggtaaca ggcccacgcg ctccagcaac cacgcaacgc
    12841 gtgcagccgc gtctttacct ggcaataaac cgtgtacacg cagcggttcg ataatcgaat
    12901 caccgatggt ctgacgtggg tccagcgaag cgtaagggtc ctgaaaaata aactgaatat
    12961 cccggcgcaa tgcctgaagt ttgccaggtg acaaggtatc gattcgctga ccgttaaaga
    13021 taatttcgcc gccctgcgat tcgaccaggc gcagcaacgc ccgcccggta gtggatttac
    13081 cgctgccaga ctcgcccacc agcgatagcg tttcgccagg ccagagatca aaactgactt
    13141 tctcaacggc atgcacttcc cgcgttacgc gattcaacaa accgctgcgc aaagggaaac
    13201 gggtgacaag attacgcacc cgtaaaacag gttcgccatc caccaccgtt ttctgctcga
    13261 tggggggttc ctgtttcgct ggatgttcaa gcgatatcaa tgggaaacgt cggggataat
    13321 ctaacccttt catcgcacca agttgcggaa cagcagctaa cagcgcacgg gtgtaaggat
    13381 gttgcggtgc atgaaaaatc tgttcgacgg tacccgtttc caccgcctcg ccctgataca
    13441 tcaccagtac ccgatcggca atctctgcca ccacgcccat atcgtgagtg ataaagataa
    13501 cgcccatcga catctctttt tgcaatactt tgattaattg caggatctgc gcctgaatag
    13561 tgacatccag cgcggtggtt ggctcatcgg caatcagcac cgccgggcgg catgacagcg
    13621 ccatcgcaat catcactcgc tggcgcatcc cgccagagag ttgatgcgaa taacgtgaaa
    13681 gaatggtttg tgcctccgga atgcgtacct gatccagcat ccgcttcgcc tcgaccatcg
    13741 cttcttcacg actggcgttc tgatgcagac gaattgattc ggcaatctgt tcacccacag
    13801 taaatactgg gttcagcgat gtcatcggct cctgaaatat catcgccata tccgcaccgc
    13861 gcacatggcg catttgtgca gcgctctgct cgctaagttc aatcacatca cgactgcgcc
    13921 gccgcaacag cattttatcg cactgtacta aaccgcccgc ctgttccaac agacgcatca
    13981 atgccagcgc agtcactgac ttaccggagc cggattcgcc tacaattgcc agcgtctcac
    14041 cgcgttgcag actaaaagag agattgcgga ccgcagctat tttctgctgg tcctgcataa
    14101 aggcaatatt aagattttca accgccagca cattaccggc atcaagttca tcactgtgtg
    14161 gcaacggtgt cccctttttc acggtagata ccggtggttg gcgtatcgcc tgcgtagccc
    14221 caggcgcgat acattccttc ggtgttaaac gatagcgcga cattcccttc atggtcgata
    14281 gcgattaagc caccgctacc gccaagcgca gggagttttt ccattactac ccgctcgcag
    14341 gcttccgcga gacttaatcc gccgtaatcc attaacgcgg cgatgtcata tgccgccagc
    14401 gcgcggatga agacttcgcc tgtgccggta caagaaaccg ccacactggc gttattggcg
    14461 tagcatccgg cacccactaa gggactatcg ccaactcgtc cgggtaattt attggtcatt
    14521 ccgcctgtgg acgtggctgc cgccagattg ccgtctaaat ccaacgccac ggcccccacg
    14581 gtgcccattt tctgtttttc atccagtggc gcaccgctat ggtcgaggac tgttgcccct
    14641 tcctcgcgcg ctgccagtag ttgttcataa cgcaaaggcg tggagaaaat ctccggtgag
    14701 acgcgctcca tgccatgagc aaacgcaaaa ttttctgccc cttcgccaat catcatcaca
    14761 tgcgggcttt gctccatcac cagccgggcg gcaagaaccg gattacgcag atgactaacg
    14821 cccgccaccg caccggcttt cagggtgtta ccatccatca cacaggcgtc cagttcatgg
    14881 gtttcatcac gcgtaaagac agcgccaatt ccggcgttaa acagtggaca ctcttccagc
    14941 agacgcaccg cttccgtcac cacatccagc gcactttcgc cggcttccag cattttctgc
    15001 ccggtttcaa caatggcaga caacgcctcg atgtagcgta attcctgttg cagactcatc
    15061 tgcgcgcggc taattgcacc tgcgccacca tgaattgcaa tgactgcttt gcccatagta
    15121 ttcgtccatt aagggtggct cgccactttt gctataaata tcagaatttt tatgaattac
    15181 tcattcaatt tttgaatata gaaagatgga tatggcatgt aaaggcagga gccaacgcta
    15241 cgcccgaggt ggcatacagt ctatggcgtt tttctgacat aataggcaaa ttcgattttg
    15301 cctccgcagg agtgttttca tggaatttac caccggattg atgtcgctcg acaccgcgct
    15361 taatgagatg ctttctcgcg ttaccccact gaccacccag gaaacgctgc cactggttca
    15421 gtgttttggt cgtattctgg cgagcgatgt cgtttcgcca ctggatgtcc cggggtttga
    15481 taactccgct atggacggct acgcggtgcg tttagccgat attgcctccg ggcaaccgct
    15541 gcccgttgcc ggtaaatcct ttgccggtca gccataccat ggtgaatggc ctgcgggtac
    15601 ctgcattcgt attatgaccg gtgcgccggt gccggaaggc tgcgaagcgg tggtgatgca
    15661 ggagcagact gaacaaacgg acaatggcgt gcgttttact gctgaagtgc gtagcgggca
    15721 aaatattcgc cgtcgcggtg aagatatctc tgcaggtgcg gttgttttcc cggcgggaac
    15781 tcgcctgact accgcagagc tgccagtaat tgcttcactg gggattgccg aagttccggt
    15841 gattcgtaaa gtgcgtgtag cgcttttttc taccggtgat gaactccagt tgcccggtca
    15901 gccgctgggc gacggccaaa tctacgacac caaccgtctc gccgtacacc tgatgttaga
    15961 acagctgggt tgcgaggtaa tcaacttagg gattatccgc gacgatccac atgccctgcg
    16021 cgccgcattt attgaagccg acagccaggc ggatgtagtg atcagttccg gcggtgtttc
    16081 agtgggtgaa gcggattaca ccaaaacgat tcttgaagag ctgggggaga tcgccttctg
    16141 gaagctggcg attaaaccag gtaaaccgtt cgcgttcggt aaactcagca atagctggtt
    16201 ctgcggcctg ccgggcaacc cggtttcagc gacgctgacc ttctatcaac tggtacagcc
    16261 tttgctggca aaactaagcg gcaacaccgc cagcggcctg cccgcgcgcc agcgcgtacg
    16321 cacagcgtcc cgcctgaaaa aaacgccagg acgtcttgat ttccagcgcg gcgtgctgca
    16381 acgcaacgcc gacggtgaac ttgaagtgac gaccaccgga catcagggtt cacatatatt
    16441 tagctccttt agtctcggca actgctttat cgtgctggaa cgcgatcgcg gcaatgtgga
    16501 cgtgggcgaa tgggtggaag tagaaccgtt taacgcgttg ttcggaggcc tgtaatggcg
    16561 gaactcagcg atcaggagat gctgcgctac aaccggcaaa tcattctgcg cggttttgat
    16621 tttgacggtc aggaggcgct aaaagattct cgtgtgctgg tagtcggcct cggtggactc
    16681 ggctgcgcag cttcgcagta tctggcaagc gccggtgtcg gcaacctgac gctgctcgac
    16741 ttcgacacgg tttcgctctc gaatctgcaa cgccagacac tgcacagtga tgccacggtc
    16801 gggcaaccga aggtggaatc cgcccgtgac gccctgacgc ggatcaaccc acatatcgcg
    16861 attacgccag tcaatgcact gctagatgac gcagaacttg cagcattgat tgctgaacac
    16921 gatctggtgc tcgactgtac ggataacgtt gcggtacgta atcaactgaa cgcaggctgt
    16981 tttgccgcga aggtgccgct ggtttccggc gcggcaattc gtatggaagg gcaaatcacg
    17041 gtcttcactt atcaggacgg cgaaccgtgc tatcgctgcc ttagccgttt gtttggcgag
    17101 aacgcgttaa cctgcgtaga agcaggcgtg atggcaccgt tgatcggcgt aattggttcg
    17161 ttgcaggcaa tggaagcgat caaactgctg gcaggttatg gcaaaccggc cagcgggaaa
    17221 atcgtcatgt acgatgcgat gacctgtcag tttcgcgaaa tgaaactgat gcgcaatccg
    17281 gggtgtgagg tgtgcgggca gtaattgcca cctccaggcc tgataagacg cgacaagcgt
    17341 cgcatcaggc atttgctcca gacgccagat gcggcgtgaa cgccttatct ggcctacggt
    17401 tcgggcacag atttccaagc gcggcaggca tcggtgcact ccgccacgtc atcagcaatt
    17461 tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc aaacgctccc tgccagtcct
    17521 gctcaaactt cgccacagcg gcttccaccg ccggatagct aatcatctgt tgtgccacat
    17581 ccagtggcag agtaattgat tcacatcctg ccagtaagca gtccagcgcc tgacgcgggg
    17641 ttttgaaact cgctgccagc actttcgcct gcggcgcatg cattttcaat aactggtgta
    17701 agtcggtcac agtctgaatg ccgctaccgc cctgagcatc aatacgatta acgtaaggcg
    17761 caacatattc cgcacctgcc agcgccgaca gcagcccttg tgccgcgcca tataccgcgg
    17821 ttcccagcgt cggaatccct tccgctttta acatcttaat cgctgccagc ccctcggctg
    17881 tcaccggaac tttcaccacg atatccgcaa taatagaacg cagcttaagc gcgtcattca
    17941 ccatcccttc agcagtggta gccattacct gggcaaacag acgcccctga ccgcccatcg
    18001 cttcatgaag ttgcggaagc acaacatcca gcggtttttt acccgcggcg ataatgcttg
    18061 ggttagtggt cacacccgcc agcggaaaaa tacgtgacag cgccttcacc gcaacaacgt
    18121 ctgaagtatc cagatacagt tccataacca tcctcaaaat gtactttttg tctgaatgta
    18181 gactatcacg ggaaaactct ctgaacagtt gacgcacatc aatctaactt tcattcgaaa
    18241 gtattttaat gtttgaacga aacagagagg caactatgat tttcaatatt cagcgctact
    18301 cgacccatga tggccccggt atccgcacgg tcgtatttct taaaggctgt tcgctgggct
    18361 gccgctggtg tcagaacccg gaaagccgcg cccgtacgca ggatctgctg tatgacgcac
    18421 gactgtgtct ggaaggctgc gagctgtgcg ctaaggccgc gccggaagtg attgagcgcg
    18481 cgctgaatgg tttgcttatt catcggaaaa agttaacccc ggagcatctg acggcgttaa
    18541 ccgactgctg tccgacacag gcgttaaccg tgtgtggtga agtgaaaagc gttgaggaga
    18601 tcatggcgac cgttctgcgc gataaaccgt tttacgatcg cagcggcggc ggcttaacgc
    18661 tttcgggtgg tgaacccttc atgcagccgg aaatggcgac ggcgctgctg caagcctgcc
    18721 acgaggcagg cattcatact gcggtagaaa cctgtctgca tgtgccgtgg aaatatatcg
    18781 ccccttctct gccctatatc gatctgtttc ttgccgattt aaaacacgtt gccgacgcgc
    18841 cgtttaaaca gtggaccgac ggtaacgccg ccagggtgct ggataacctg aaaaaactcc
    18901 ccgcagcggg caaaaaaatc attatccgcg tgccgctgat tcagggcttt aatgccgacg
    18961 aaacctctgt aaaagccatt accgattttg ccgccgacga gctgcacgtt ggcgaaattc
    19021 attttctgcc ctaccacacg ctgggcatca acaaatatca cttacttaat ctgccctatg
    19081 acgccccgga aaaaccgctt gatgcgccag agctgctcga ttttgcccag cagtatgcct
    19141 gccagaaagg gttaaccgcg accttacgag gataacaacc atgaccacac tgaaactgga
    19201 cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg gtgcatattg tgaaaccgcc
    19261 agtctgtacc gagcgcgcgc agcactatac cgagatgtat caacaacatc tcgataagcc
    19321 gatcccggta cgtcgcgcgc tggcactggc gcatcacctg gcgaatcgca ccatctggat
    19381 caaacacgat gagttgatca ttggcaacca ggcaagcgaa gttcgcgccg cgccgatctt
    19441 cccggaatat actgtctcgt ggatcgaaaa agagattgat gatctggcag atcgtcccgg
    19501 tgctggcttt gcggtgagcg aagagaacaa acgtgttctg catgaagtgt gcccgtggtg
    19561 gcgcggtcag acggtacagg atcgctgcta tggcatgttt accgatgagc aaaaaggtct
    19621 gctggcgacc ggcatcatta aagcggaagg caatatgacc tccggcgatg cgcacctggc
    19681 ggtcaatttc ccgctgctgc tggaaaaagg gcttgatggt ctgcgtgaga aagtagcgga
    19741 acgtcgctcg cgcatcaatc tgacggtgct ggaagatttg cacggcgaac agttcctgaa
    19801 agcgattgat atcgtgctgg tggcagtcag tgaacacatt gaacgtttcg ctgccctggc
    19861 gcgtgcaatg gccgcgaccg aaacccgcga aagccgtcgc gatgaactgc tggcgatggc
    19921 agaaaactgc gatcttatcg cccaccagcc gccgcagact ttctggcagg cgctgcaact
    19981 gtgttacttc atccagttga ttttgcagat tgaatctaac ggtcactcgg tgtcgtttgg
    20041 tcgtatggat cagtatctct acccgtacta tcgccgcgac gttgaactga accagacgct
    20101 ggatcgcgaa cacgccatcg aattgctgca tagctgctgg ttgaaattgc tggaagtgaa
    20161 caaaatccgc tccggctccc actcaaaagc ctctgcggga agtccgctgt atcagaacgt
    20221 caccattggc ggacaaaatc tggttgatgg tcaaccaatg gacgcggtga atccactctc
    20281 ttacgcgatc ctcgaatcct gcggtcgcct gcgttccact cagcctaacc tcagcgtgcg
    20341 ttaccatgca ggaatgagta acgatttcct cgacgcctgc gtacaggtga tccgctgcgg
    20401 cttcgggatg ccggcattca acaacgacga aatcgtgatc ccggaattta ttaaactcgg
    20461 tattgaaccg caggacgctt atgactacgc agcgattggt tgtatagaaa ccgccgtcgg
    20521 tggcaaatgg ggctatcgct gtaccggcat gagctttatc aacttcgccc gcgtgatgct
    20581 ggcggcgctg gaaggcgggc gtgatgccac cagcggcaaa gtgttcctgc cacaagaaaa
    20641 agcgttgtcg gcaggtaact tcaacaactt cgatgaagtg atggacgcgt gggatacgca
    20701 aatccgttac tacacgcgta aatcaatcga aatcgaatat gtcgtcgaca ccatgctgga
    20761 agagaacgtg cacgatattc tctgctcggc gctggtggat gactgtattg agcgagcgaa
    20821 aagtatcaag caaggcggcg cgaaatatga ctgggtttct ggcctgcagg tcggtattgc
    20881 caacctcggc aacagcctgg cggcagtgaa gaaactggtg tttgaacagg gcgcgattgg
    20941 tcagcaacag ctggctgccg cactggcgga tgacttcgat ggcctgactc atgagcaact
    21001 gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac gacgatgata ccgtcgatac
    21061 gctgctggct cgcgcttatc agacctatat cgacgaactg aaacagtacc ataacccacg
    21121 ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt acgtcgtcta tctccgctaa
    21181 cgtaccgttt ggcgcgcaaa ctatggcaac gccggacggg cgtaaagccc acaccccgct
    21241 ggcagaaggc gctagtccgg cctctggtac tgaccatctc ggccctactg cggtcattgg
    21301 ctcagtgggt aaactgccta cggcagcgat tctcggcggc gtgttgctca accagaaact
    21361 gaatccggca acgctggaga acgaatctga caagcagaaa ctgatgatcc tgctgcgtac
    21421 cttctttgaa gtgcataaag gctggcatat tcagtacaac atcgtttccc gcgaaacgct
    21481 gctggaggcg aaaaaacatc cggatcagta ccgcgatctg gtagtacgtg tcgcgggtta
    21541 ttccgccttc ttcaccgcgc tctctccaga cgctcaggac gatatcatcg cccgtactga
    21601 acatatgctg taatctcttc ggcccggctg cggcgtcgcc gggctaaatc attcacatca
    21661 tcaatttcat ccttactttc attcgaaata taatttgtgc tctgcgtcac attgttatta
    21721 cactgttacg gtttcatttt cgagccagga gcacacctat gagcgtaaaa gttatcgtca
    21781 cagatatgga cggtactttt cttaacgacg ccaaaacgta caaccaacca cgttttatgg
    21841 cgcaatatca ggaactgaaa aagcgcggca ttaagttcgt tgtcgccagc ggtaatcagt
    21901 attaccagct tatttcgttc tttcctgagc taaaggatga gatctctttt gtcgcggaaa
    21961 acggcgcact ggtttacgaa catggcaagc aactgttcca cggcgaactg acccgacatg
    22021 aatcgcggat tgttattggc gagttgctaa aagataagca actcaatttt gtcgcctgcg
    22081 gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc atttgtcgca ctgatggcaa
    22141 aacactacca tcgcctgaaa cctgtaaaag attatcagga gattgacgac gtactgttca
    22201 agttttcgct caacctgccg gatgaacaaa tcccgttagt gatcgacaaa ctacacatag
    22261 cgctggatgg cattatgaaa cccgtcacca gtggttttgg ctttatcgac ctgattattc
    22321 ccggtctaca taaagcaaac ggtatttcgc ggttactgaa acgctgggat ctgtcaccgc
    22381 aaaatgtggt agcgattggc gacagcggta acgatgcgga gatgctgaaa atggcgcgtt
    22441 attcctttgc gatgggcaat gctgcggaaa acattaaaca aatcgcccgt tacgctaccg
    22501 atgataataa tcatgaaggc gcgctgaatg tgattcaggc tgtgctggat aacacatccc
    22561 cttttaacag ctgacctctc taccggagcc atgcgctccg gttttccctt ctttttacat
    22621 cctgtacatc acatcaaatc attaaacttg cattaactta tttataactt gtattgtcac
    22681 atgcaaatca gcatagtttc gaatgaaagt taatgcgagg tggttatggc ttctactttt
    22741 accagcgaca cattgcctgc cgatcacaaa gcagctatcc gtcagatgaa gcacgcgctg
    22801 cgggcgcagc ttggcgatgt ccagcagatc tttaatcagc taagcgatga cattgccacg
    22861 cgagtggctg aaatcaacgc actcaaagca cagggcgatg ccgtctggcc ggtgctgtct
    22921 tatgccgata tcaaagcagg tcatgttact gcagagcagc gcgaacagat taaacgtcgc
    22981 ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag cgctaggctg ggatcagtcg
    23041 atgctggact atctggaccg caaccgcttt gacgaggtct acaaaggccc cggcgataat
    23101 ttcttcggga cgctcagcgc ttcacgtccc gagatttacc ccatctactg gtcgcaggcg
    23161 caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc agtcgtttct caatcgtctg
    23221 tggacatttg aaagtgatgg aaagcaatgg tttaacccgg atgtgagcgt catctaccct
    23281 gaccgtatcc gccgccttcc gcccggaacg acctccaaag gtcttggagc gcataccgac
    23341 tccggggcgc tggaacgctg gctgcttcca gcatatcagc acgttttcgc caacgtcttt
    23401 aatggcaatc tggcgaaata cgatccctgg catgcggcac atcgtacgga agttgaagag
    23461 tacacggtgg acaacaccac caaatgttcc gtgtttcgga cattccaggg ctggactgcg
    23521 ctctcggata tgctgcctgg tcaggggttg ctgcacgtcg tgcccattcc tgaagccatg
    23581 gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg aggatgaact gtgcggcgta
    23641 gcgcccggaa gagtgttgcc ggtatcagag caatggcatc cactgttaat tgaggcgtta
    23701 accagcattc caaaactcga ggccggagac tccgtctggt ggcactgcga cgtcatccat
    23761 tccgttgccc ccgttgaaaa tcaacagggt tggggcaacg tgatgtacat tcctgcggca
    23821 ccgatgtgcg agaaaaatct tgcctacgcg cacaaggtga aggccgcact ggaaaaaggc
    23881 gcatcgccgg gcgacttccc gcgcgaggac tatgaaacaa actgggaagg acgctttacg
    23941 cttgccgacc tcaacattca cggtaagcga gcgttgggca tggatgtttg agagttaatg
    24001 gaagtgaatt gatgtgggat taagaaaatc gattcgacgt tactcagatt gctgacaagt
    24061 gcgctttctt aatgcctgat gcggcgttaa cgccttatcc ggcctacaaa gtcctgcaga
    24121 ttcaatatat tacaggtact gcgtaggcct gataagcgta gcgcatcagg ctattttgca
    24181 tttgtcatca gccgtcgtca ccccgacagt atcggggtga cggataatca cttactcgat
    24241 ccctttacta cgcaggtaat cttcgtaatt accgctaaag tcgatcacgc gttccggggt
    24301 gatttccaga atgcgggtcg ccagggagct tacgaactca cggtcgtgtg aaacaaagat
    24361 cagcgtgccc tgatacagtt ccagcgccat gttcagtgac tcgatggatt ccatatccag
    24421 gtggttggtc ggttcgtcca tgatcagaat gttcggcttc tgcatcatta acttaccaaa
    24481 cagcatccgc cctttttccc caccggaaag cactttagct ggctttttga tgtcgtcctg
    24541 gctgaacagc aaacgaccga gaatgctgcg taccgcctgc tcgtcatcgc cttcctgctt
    24601 ccactgactc atccattcga acacggtcag atcattttca aactcatatt cgtggtcctg
    24661 ggcgtagtaa ccaatgcgcg cgttttcaga ccattttacg gtgccgctgt ccggttgcag
    24721 atcgcccacc agcgttttca gcagcgttga tttaccgacg ccgttggtac ccagtaccgc
    24781 aagtttttca ccgacctcca gcagcaggtt gagattttta aacagtggac cgttatcaaa
    24841 ccctttggtc agaccttcca cttccagcgc gttacggaac agtttcttat cctgttcaaa
    24901 acggatgaac gggttctgac ggctggaggc tttcacctct tccagtttga ttttatcaat
    24961 ctggcgcgcg cgcgaagttg cctggcgaga tttcgaggcg ttggcgctaa agcggctaac
    25021 gaaagattgc aactcagcaa tctgcgcttt cttcttggcg ttatcggcca gcagacgttc
    25081 acgcgcctgg gtcgccgccg tcatgtactc atcgtagtta cccggataaa cgcgcagctc
    25141 gccgtaatcc agatccgcca tgtgggtaca gaccatgtta aggaagtgac ggtcgtgcga
    25201 gatgatgatc atggtgctgt cacgctcgtt cagcacctgt tccagccagc gaatggtgtc
    25261 gatgtcgagg ttgttggtcg gttcgtcgag caggagaata tccggctcag caaacagcgc
    25321 ctgcgccaga agcacacgca gcttccagcc aggagcaact tcactcatcg ggccgtagtg
    25381 ctgttccact ggaattccca cgccaagcaa cagttcaccg gcgcgagctt ccgcagagta
    25441 accgtccatt tcgccgtatt tcacttccag atcggccact ttatagccgt cttcttcact
    25501 catttccggc aaagcataga tgcggtcgcg ctcctgcttc acttcccaca actctttatg
    25561 ccccatgatc accgtatcca gcacagtgaa ctcttcaaag gcaaactgat cctgacgcag
    25621 cttaccaatg cgctcgttgg gatcgaggga aacgttaccc agcgtcggct caaggtcgcc
    25681 gccgagaatc ttcataaagg tggatttacc actaccgttc gcgccaatca ggccgtaacg
    25741 gttgccgccg ccgaatttga cggaaatgtt ttcaaataac ggcttactgc cgaactgcat
    25801 ggtgacgtta ctggaaacta acacaggcgt atcctgaaaa gagatatgac aaaccgcgca
    25861 ttatgccata agtctgaaag aagatcctgc tcctcgctta aggtctacac tcaagccaaa
    25921 ggcaaaaaat gtgatttcgt acacatctga tttcactgtg agctggaatg aacttataat
    25981 gcgcttccaa tactctaata ttctcaaccc aatggcctgc caggcacaaa atctcgctta
    26041 acatgaatat gaaattgaaa acattattcg cagcggcctt cgctgttgtc ggcttttgca
    26101 gtaccgcctc tgcggtaact tatcctctgc caaccgacgg gagtcgcctg gttggtcaga
    26161 atcaggtgat caccattcct gaaggtaaca ctcagccgct ggagtatttt gccgcggagt
    26221 accagatggg gctttccaat atgatggaag cgaacccggg tgtggatacc ttcctgccga
    26281 aaggcggtac tgtactgaac attccgcagc agctgatcct gccggatacc gttcatgaag
    26341 gcatcgtcat taacagtgca gagatgcgtc tgtattacta tccgaaaggg accaacaccg
    26401 ttatcgtgct gccgatcggc attggtcagt taggcaaaga tacgcctatc aactggacca
    26461 ccaaagttga gcataagaaa gcaggcccga cctggacgcc gaccgccaaa atgcacgcag
    26521 agtaccgcgc tgcgggcgaa ccgctaccgg ctgtcgttcc ggcaggtccg gataacccga
    26581 tgggtctgta tgcactctac atcggtcgcc tgtatgctat ccatggcacc aacgccaact
    26641 tcggtatcgg cctgcgtgta agtcatggtt gtgtgcgtct gcgtaacgaa gacatcaaat
    26701 tcctgttcga gaaagtaccg gtcggtaccc gcgtacagtt tattgatgag ccggtaaaag
    26761 cgaccaccga gccagacggc agccgttata ttgaagtcca taacccgctg tctaccaccg
    26821 aagcccagtt tgaaggtcag gaaatcgtgc caattaccct gacgaagagc gtgcagacag
    26881 tgaccggtca gccagatgtt gaccaggttg ttcttgatga agcgatcaaa aatcgttccg
    26941 ggatgccggt tcgcctgaat taatcgtcaa aacgtaaaac aaaggcggac tcataatccg
    27001 ccttttttat ttgccagacc ttaccaggcc gggatcataa ctaacaaaac gtatcccacc
    27061 agcgccgccc acaacagcat cggtattgaa tagagatgga aacgccacca gatgcggcga
    27121 tcgttcgcca tacgtagcgc aatcaaattt gccagcgatc ctggtaataa accaaagccc
    27181 cccacgttta ccgcccatgc cagtaataat aaagacggag gtacataatt gagcagcaaa
    27241 atggtactcg gcacgttact gatcacctgc gataaaccga ctgccgttaa ccataacccg
    27301 ggttcagata aatgactcac gttacccaac acgccttgca acgctggaag ctgggtcagt
    27361 aaatggacgt cgataaacat cgccataaac accagcagca acgtccagtc cacactcaac
    27421 accacgcgac gcgccagcag cacaaagccc accgccacaa tcaccagtcc ccacagctct
    27481 tgtttgaact ccagcgccgt cagaaagacg atatacagcc ccagacaatt ccacaccagc
    27541 cgcggtttcc actccggtgt ttgcaccccc gtatggtatt gcagcgcctt tccagggaaa
    27601 caacaccagc acaacagcag gagtgtcagc atcattgcgc cagccagcgg tgccatttgg
    27661 gcaataaatc cggcaaacga aagaccagaa cgtccccaga taagaatatt ttgcgggttg
    27721 ccaattggcg tcagtagcga accagcgttg actgccagcg cctcaaaaat aatcagccga
    27781 ttaaccggga tctcacacag tctttttagc gtgatagtca gcggaacaac aataaacagc
    27841 gcgacatcgt tggtcagaaa ggtagaaagc agcgccgccg ccagcaccat aaacatcgcc
    27901 agccgacgct ccgtagcaaa gcggcgcacc attttgcgcc ccagcacatc aaaataaccg
    27961 cttaactcca cacctttggt cagcagcatc aggccgctta aggtgatgat ggtgtgccag
    28021 tcgatagcag caggccagga tttcggtgca aagggcacga aaaagcttaa tccgatacca
    28081 acaagaatta ataactgaaa aaaacgatcg ccttgcagcg tgcgtaaaaa aggcaggctc
    28141 atttggcacc gtgtttctgg gtaaacaaac gaaaagcgtc gagcgtctct tcactaacat
    28201 ggtgctccat gccttccgcg tcgcgacggg cgatttccgg actgacgccc aacaccaaca
    28261 agaaattttc gactatctga tgtcgctcgc ggctttcctg cgccagcttc tctccttctg
    28321 ccgttaaaaa cacgccacgc caggggatca tttcaatcag ccccatggtt gccagccgct
    28381 taagcatttt agccaccgtc ggttgcgaaa ctcccagacg agcagccatg tccacctgac
    28441 gagcttcccc cacttccctg atcaagtcag aaatcagctc aacgtaatca tcaataagct
    28501 cgcgccgatg cgcctctctg acctggcgga acccttcaac gtgctcttcc acgttcacta
    28561 actgcgtcac tttttttgct gttggcgtac ctgcgcgacg actcattgtg cttcctcatt
    28621 atggtgacgc atacatagcg ttctcatttc gtgcgcacat tgtaaaccag agttgcgaag
    28681 gtacaaaaaa ttaacgtttt agcaatagct atataatata gcctgtgcta tatctgtatg
    28741 taatgcaatc atccctcaag gatcgacggg attagcaagt caggaggtct tatgaatgag
    28801 ttcaagaggt gtatgcgcgt gtttagtcat tctcccttta aagtacggtt aatgctgctc
    28861 tctatgttgt gcgatatggt caacaacaaa ccgcagcaag ataaaccttc agataaatag
    28921 cggcgtcgcg gtacgccgct tcactcctgc ttttatgcag gcataacgcg ttttggtctg
    28981 aaaaaaccca ctttttgtcg gatttgcaat ccccttcgca aaagatttgt tcgtcagtag
    29041 ttgacctgaa cggcggctcg ctctatcttc ttgcagccct gcgtatattg cggctcgcgg
    29101 atgcggaccc ctttcccctc ttcacgcact cttgcaggta ttgacccttg acgccagggt
    29161 aagcacatgg cgtttgttac gatagtggca tatgaattta accctcaaag aatcgcttgt
    29221 tacccgtagc cgggtattta gcccgtggac tgcgttctac tttttacagt cgctattaat
    29281 taacctcggc ttaggttacc ccttcagttt gctctacacc gctgcgttta cggctatttt
    29341 gcttttgcta tggcgaacat tgcctcgcgt acaaaaagtt ctggtcggtg tcagttcgct
    29401 ggtggcggct tgttatttcc cttttgctca ggcctacggc gcgcctaatt tcaatacatt
    29461 gctggcattg cactccacca atatggaaga gtcgaccgaa atcctgacga tttttccgtg
    29521 gtacagctac ctggtcggct tatttatttt tgcgctcggc gtaatagcaa tcaggcgaaa
    29581 aaaagagaat gaaaaagcgc gctggaatac cttcgacagc ctgtgtctgg tattcagtgt
    29641 ggcgacattt tttgttgctc ccgtgcaaaa cctggcctgg ggtggcgtat ttaaactgaa
    29701 agatactggc tatccggtat ttcgttttgc taaggatgtc atcgtcaata ataacgaggt
    29761 gattgaagag caagaacgga tggcaaaact ttccggaatg aaagatactt ggacggtcac
    29821 tgccgttaag ccgaagtatc agacctatgt ggtagtgatc ggtgaaagcg cgcgtcgcga
    29881 tgccctcggt gcctttggcg gtcactggga caataccccg tttgccagca gcgttaacgg
    29941 tttgatattt gctgactaca ttgccgccag tggctccacg cagaaatcgc ttggcttaac
    30001 gctcaatcgc gtagtcgatg gcaaaccaca gtttcaggat aactttgtca ccctggcaaa
    30061 tcgcgcgggc ttccagacct ggtggttttc caaccagggc caaatcggcg aatacgatac
    30121 tgctatcgcc agcatcgcca aacgtgcaga tgaagtgtac ttcctgaaag aaggtaattt
    30181 tgaagcagat aaaaacacga aagacgaagc gttactggat atgaccgctc aagtgctggc
    30241 gcaagagcac tcgcaaccgc agctgattgt tctgcatctg atgggctcgc atccgcaggc
    30301 ctgcgacagg acacaaggca aatacgaaac ctcctttgtg caatcgaaag aaacgtcgtg
    30361 ctatctctat accatgacgc aaacggacga tttactgcgc aagctgtacg atcagttacg
    30421 caacagcggc agcagcttct ctctggttta cttttctgac cacggtctgg cctttaaaga
    30481 gcgcggcaaa gacgtgcaat accttgccca tgatgataaa tatcagcaaa atttccaggt
    30541 gccttttatg gtcatttcca gcggcgataa agcgcatcgg gtgattaaag cccgccgctc
    30601 agccaatgac ttcttaggct ttttctcgca gtggacggga attaaagcga aggaaataaa
    30661 tatcaaatac ccgtttatat ctgagaagaa agccgggccg atatacatca ccaacttcca
    30721 gttacagaag gtggattaca accatctcgg aaccgatatt ttcgacccga aaccttaaaa
    30781 acaaaaatcc gccccgagag gcggattttt tatatcacca aagtgattag aagcggtaac
    30841 caacaccggc aatccaggtg cctacgtcaa cgctacgaat acggctctgc tcgtaagaga
    30901 agtccagagc aacgttttcc atcgggttga actgcagacc agcaccgtag gagaaaccgt
    30961 agtcgctggt gtcgtgtttg taggtcgggt attcagtggt ctggaattta ccataaccca
    31021 cacccactac accgtagatg cttgcccagt cgttaatgcg gtaagccgga ccagcagtga
    31081 tgccatagta ctggtttttg ttgtagtcac cagagcttgc agtacggctt ttctcggtgt
    31141 aagtgaaaga accgatcaca cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt
    31201 tgaaaccgcc cattttgttc atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta
    31261 cggtagaagt cgcagctacg gaagtacctg cggtgaaagc cagaactgcg gccagtgctg
    31321 aaagacatgc aatttttttc ataaccacct caaatgtgat tcaaataagt cctaagtttt
    31381 aaatatatca aaaattaatg ggaaactctt cgcgatttgt gatgtctaac gggccatttc
    31441 atgtaacaga acgtttccat acaccgctat ccatctaaat ttaaatcact ttttcagaga
    31501 actgcgtaag tattacgcat gttttccctg tcattcatcc agattattcc taatcaccag
    31561 actaatgatt ccatcaatcc tggcgcattt tagtcaaaac gggggaaaat tttttcaaca
    31621 aatgctcaac cagcattggg tatatccagt acactccacg ctttacttaa gtctagatat
    31681 ttgtgggaga aaggatgcct ggttcattac gtaaaatgcc ggtctggtta ccaatagtca
    31741 tattgctcgt tgccatggcg tctattcagg gtggagcctc gttagctaag tcactttttc
    31801 ctctggtggg cgcaccgggt gtcactgcgc tgcgtctggc attaggaacg ctgatcctca
    31861 tcgcgttctt taagccatgg cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt
    31921 tttacggcgt ttcgctgggt gggatgaatt atctttttta tctttctatt cagacagtac
    31981 cgctgggtat tgcggtggcg ctggagttca ccggaccact ggcggtggcg ctgttctctt
    32041 ctcgtcgccc ggtagatttc gtctgggttg tgctggcggt tcttggtctg tggttcctgc
    32101 taccgctggg gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg
    32161 ccggggcttg ttgggctatt tacattttaa gtgggcaacg cgcaggagcg gaacatggcc
    32221 ctgcgacggt ggcaattggt tcgttgattg cagcgttaat tttcgtgcca attggagcgc
    32281 ttcaggctgg tgaagcactc tggcactggt cggttattcc attgggtctg gctgtcgcta
    32341 ttctctcgac cgctctgcct tattcgctgg aaatgattgc cctcacccgt ttgccaacac
    32401 ggacatttgg tacgctgatg agcatggaac cggcgctggc tgccgtttcc gggatgattt
    32461 tcctcggaga aacactgaca cccatacagc tgctggcgct cggcgctatc atcgccgctt
    32521 caatggggtc tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gacattaatt
    32581 aaatttacat ttctgtatgg ttatgcataa ccatgcagaa tttctcgcta cttttcctct
    32641 acaccgtctt tatatatcga attatgcaaa agcatattta ttccgaaaat tcctggcgag
    32701 cagataaata agaattgttc ttatcaatat atctaactca ttgaatcttt attagttttg
    32761 tttttcacgc ttgttaccac tattagtgtg ataggaacag ccagaatagc ggaacacata
    32821 gccggtgcta tacttagtct cgttaattac tgggacataa catcaagagg atatgaaatt
    32881 atgagtaccg ctaaattagt taaatcaaaa gcgaccaatc tgctttatac ccgcaacgat
    32941 gtctccgaca gcgagaaaaa agcaacagta gagttgctga atcgccaagt tatccagttt
    33001 attgatcttt ctttgattac caaacaagcg cactggaaca tgcgcggcgc taacttcatt
    33061 gccgtacatg aaatgctgga tggcttccgc accgcactga tcgatcatct ggataccatg
    33121 gcagaacgtg ccgtgcagct ggacggtgta gctctgggga ccactcaagt tatcaacagc
    33181 aaaaccccgc tgaaaagtta cccgctggac atccacaacg ttcaggatca cctgaaagaa
    33241 ctggctgacc gttacgcaat cgtcgctaat gacgtacgca aagcgattgg cgaagcgaaa
    33301 gatgacgaca ccgcagatat cctgaccgcc gcgtctcgcg acctggataa attcctgtgg
    33361 tttatcgagt ctaacatcga ataaatccat cgctgatggt gcagaacttt agtacccgat
    33421 aaaagcggct tcctgacagg aggccgtttt gttttgcagc ccacctcaac gcacttattt
    33481 agtgcatcca tctgctatct ccagctgatt aagtaaattt tttgtatcca catcatcaca
    33541 caatcgttac ataaagattg ttttttcatc aggttttacg ctaaataatc actgtgttga
    33601 gtgcacaatt ttagcgcacc agattggtgc cccagaatgg tgcaccttca gggtattgcc
    33661 ctataaatcg tgcatcacgt ttttgccgca tctcgaaaaa tcaatgagtt gcaaaactgg
    33721 cacgattttt tcatatatgt gaatgtcacg caggggatcg tcccgtggat agaaaaaagg
    33781 aaatgctatg aagtctgtat taaaagtttc actggctgca ctgaccctgg cttttgcggt
    33841 ttcttctcat gccgcggata aaaaattagt tgtcgcgacg gataccgcct tcgttccgtt
    33901 tgaatttaaa cagggcgata aatatgtggg ctttgacgtt gatctgtggg ctgccatcgc
    33961 taaagagctg aagctggatt acgaactgaa gccgatggat ttcagtggga tcattccggc
    34021 actgcaaacc aaaaacgtcg atctggcgct ggcgggcatt accatcaccg acgagcgtaa
    34081 aaaagcgatc gatttctctg acggctacta caaaagcggc ctgttagtga tggtgaaagc
    34141 taacaataac gatgtgaaaa gcgtgaaaga tctcgacggg aaagtggttg ctgtgaagag
    34201 cggtactggc tccgttgatt acgcgaaagc aaacatcaaa actaaagatc tgcgtcagtt
    34261 cccgaacatc gataacgcct atatggaact gggcaccaac cgcgcagacg ccgttctgca
    34321 cgatacgcca aacattctgt acttcatcaa aaccgccggt aacggtcagt tcaaagcggt
    34381 aggtgactct ctggaagcgc agcaatacgg tattgcgttc ccgaaaggta gcgacgagct
    34441 gcgtgacaaa gtcaacggcg cgttgaaaac cctgcgcgag aacggaactt acaacgaaat
    34501 ctacaaaaaa tggttcggta ctgaaccgaa ataataacgc tacacctgta aagcgcactg
    34561 gcagttccct ctcccccctg gggagaggat tagggtgagg ggcgcaaacc cgctccgggg
    34621 ccattaatta ccctgaattt gattatttac accacggtaa caggaacaac atatgcagtt
    34681 tgactggagt gccatctggc ctgccattcc gcttctgatt gaaggtgcca aaatgaccct
    34741 gtggatttcg gtcctcggtc tggctggcgg tctggtaatc ggattgctgg caggttttgc
    34801 acgcaccttc ggtggttgga tagccaacca cgtcgcgctg gtctttattg aagtgatccg
    34861 cggcacacct atcgtcgtcc aggtgatgtt tatctatttc gccctgccga tggcgtttaa
    34921 cgacttacgc atcgacccat ttactgcggc ggtggtcacc atcatgatca actccggcgc
    34981 gtacattgcg gaaatcacgc gtggtgcggt gctgtctatc cacaaaggtt ttcgtgaagc
    35041 aggactggcg ctcggtcttt cacgttggga aaccattcgc tacgtcattt taccgctggc
    35101 actgcgtcgt atgctgccgc cgctgggtaa ccagtggatc atcagcatta aagacacctc
    35161 gctgtttatt gttatcggcg tggcggaact gacccgtcag gggcaagaaa ttattgccgg
    35221 taacttccgc gcccttgaga tctggagcgc cgtggcggtg ttctatctga ttattaccct
    35281 ggtgctgagc tttattctgc gtcgtctgga aagaaggatg aaaatcctgt gattgaattt
    35341 aaaaacgtct ccaagcactt tggcccaacc caggtgctgc acaatatcga tttgaacatt
    35401 gcccagggcg aagtcgtggt gattatcggg ccgtccggtt ccggtaaatc gaccctgctg
    35461 cgctgcatca acaaactgga agaaatcacc tccggcgatc tgattgtcga tggcctgaag
    35521 gttaacgatc cgaaagttga cgagcgcctg attcgccagg aagcaggtat ggtgttccag
    35581 cagttttacc tcttcccgca tctgacggcg ctggaaaacg tcatgtttgg tccgctacgc
    35641 gtgcgtggcg cgaacaaaga agaggcggaa aaactggcgc gtgagctgct ggcgaaagtc
    35701 ggtctggcag aacgtgcaca tcactaccct tccgaacttt ctggtggtca acagcagcgt
    35761 gtggcgattg cccgcgcgct ggcggtgaag ccgaaaatga tgctgtttga tgaaccgact
    35821 tccgctcttg acccggaact gcgccatgaa gtgctgaagg ttatacagga tctggctgaa
    35881 gaagggatga cgatggtgat cgtgacccac gaaatcggtt ttgccgagaa agtagcttcg
    35941 cggctgatct ttatcgacaa aggccggatt gcggaagatg gcaatccgca ggtgttgatc
    36001 aagaacccgc cgagccagcg cttgcaggaa tttttgcagc acgtctctta ataagacaca
    36061 ttgcctgatg cgctacgctt atcaggccta caggatatct ggcaacttat taaaattgca
    36121 tgaacttgta ggacggataa ggcgttcacg ccgcatccgg caaaaaagcc cgcacgttgt
    36181 cagcaacctg cttaatatcc cttcctccct ttcacccgaa agggaggcac accagattcc
    36241 tctcatttaa aatcgcccct cccccagcat ctatacttat ctttttgctc tgttttctca
    36301 ctggaggagt catgcggtgg atcctgttca tcctcttctg cctgctgggc gcacctgccc
    36361 acgcggtatc catacccggc gttacaacca caacgacaac ggactcaacg actgaaccgg
    36421 ccccggaacc ggatatcgaa caaaaaaaag cggcctatgg cgcactggcg gatgtgctgg
    36481 ataacgacac ctcgcgtaaa gagttgatcg accagttgcg tactgtcgcc gcgacgcccc
    36541 ctgcggaacc ggtcccgaag atcgtgccgc cgacgctggt tgaagagcaa accgtgctgc
    36601 aaaaggtcac cgaagtcagc cgccattatg gtgaagccct ttccgcccgc ttcgggcaac
    36661 tttatcgcaa tatcaccggc tccccgcata agccatttaa tccacaaacc ttcagcaatg
    36721 cgctgagcca tttttcaatg ttagcggtat tagtgtttgg tttttactgg ctgattcgcc
    36781 tgtgcgcact gccgctgtat cgcaaaatgg gtcagtgggc gaggcaaaaa aatcgtgagc
    36841 gcagcaactg gttgcagctt ccggcgatga ttattggggc gtttattatc gacctgctgt
    36901 tactggccct gacattattt gtcggccagg tattaagcga caacctgaat gcaggcagtc
    36961 gcaccatcgc tttccaacaa agtttgtttc tcaacgcctt tgccctgatt gaatttttca
    37021 aagccgtact acgcctgatt ttttgcccaa acgtggcgga gctgcgcccg ttcacgattc
    37081 aggacgagag cgcccgttac tggagtcgtc gcctgagctg gttaagcagc ctgattggct
    37141 atggcctgat tgtggccgtg ccgattatct ctaatcaggt gaatgtacag ataggtgcgc
    37201 tggcgaacgt catcattatg ctgtgcatga ccgtctgggc gttgtacctg atcttccgaa
    37261 ataaaaaaga gattacccag catttgctca actttgcgga gcattcgctg gcttttttca
    37321 gcctgtttat ccgcgccttt gcgctggtgt ggcactggct ggcaagcgcc tattttatcg
    37381 tgctgttttt cttttcgttg ttcgatccgg gcaacagtct gaaatttatg atgggtgcaa
    37441 cggtgcgcag cctggcgatt attggtatcg cagcgtttgt ttccggtatg ttttcccgct
    37501 ggctggcgaa aaccatcacc ctctcgccac atactcagcg taactatccg gagctacaaa
    37561 aacggctgaa tggctggctt tcggcggcgc tgaaaacggc gcgtattctg accgtctgcg
    37621 tggcagtaat gttgctgttg agcgcatggg gattgttcga tttctggaac tggctgcaaa
    37681 acggcgcggg gcagaaaacc gtagatatcc tgatccgcat cgcactcatt cttttcttct
    37741 cggcggttgg ctggacggtg ctcgccagtt tgatcgaaaa ccggctggct tcggatattc
    37801 atggccgccc gctacccagc gcccgtacgc gtaccctgct gacgctgttc cgtaacgcgc
    37861 tggcggtgat tatcagtacc atcaccatca tgattgtgtt gtcggaaatc ggcgtcaata
    37921 tcgcgccatt gctggcaggt gccggggcat taggtctggc tatctcgttt ggttcgcaaa
    37981 cgctggtaaa agatattatc accggggtat ttattcagtt tgaaaacggc atgaacactg
    38041 gagatttggt gactatcggg ccgttgaccg gcacagtgga acggatgtcg attcgctccg
    38101 tgggcgtgcg ccaggatacc ggggcgtatc acatcattcc gtggtcttcg ataaccacct
    38161 ttgctaactt cgtccgcggt attggttcgg tagtggcaaa ctacgatgtt gatcgccatg
    38221 aagatgctga taaagccaat caggcactga aagatgcggt aacggaattg atggaaaacg
    38281 aagaaattcg cgggctgatt attggtgaac cgaactttgc cgggattgtc ggcttaagca
    38341 ataccgcgtt tacactgcgt gtttcgttca ccacgctgcc actcaaacag tggacggtac
    38401 gctttgccct cgacagccag gtgaaaaaac atttcgacct ggcgggcgtt cgcgcgccag
    38461 tgcagactta tcaggtgctg cctgctccgg gcgctccccc ggctgaaccg ctgccgccgg
    38521 gggagccaac gctttaacgt tggcgattaa caaaacggcg gcgctgctcg tcgttcataa
    38581 aggtccaggc aataaagcga ctctgctttt gcccctgggc catctctttt tttaccacct
    38641 tcaccgcgcc cacgtcggtc agagcacgat acaacggcgg taagttttca ccacgagata
    38701 ccagcgaggt aaaccacatc acctgcttcg cgaagccttt gctctcttca atcatctttt
    38761 tgataaagtt gacttcaccg ccttcacacc acaactcctg ttgctgacca ccaaagttca
    38821 gtgcatcgtc tttgttcagc cccaggttac ggcgtttacg ctcactacct gcccgcgccg
    38881 cagcggcgga atcgtggaat ggcgggttac acaaggtcgc gtcgtattgc tcgtttttgt
    38941 ggatgatgcc gttaaaaatc gccccactct ctttttgccg acgcagacga atggcgcggt
    39001 taagccccgg attagcactg ataatcgcct gcgcactgct taacgcctgg ctgctggttt
    39061 cactaccggt aaaacgccag ccatattcat gtacgccaat cagcggataa atacagttcg
    39121 caccaacgcc gatatccaga atgctggcat tcgctggaat tgttccgctt gcttcagcca
    39181 gtaaatcggc aaggtgatga atataatccg cccggcccgg tactggtggg cagagaaaac
    39241 cgtcggggat atcccagttc gctacggcgt aaaaatgggc cagcaacgcc ttattgagcg
    39301 ccttcaccgc cagcggattg gcaaagtcta cgctttgctc ccctgcgggt gtaagcgtga
    39361 ggaattgcct gagttcagga ttgacctgac aaagcgtggc gagatcgtag cggctgtgat
    39421 gacggttgcg cggatgcaac cccggtttct gggcggacat agcattctcc tttaagcagc
    39481 ggcgtaagat acccgcataa cgcgacaggg taaatatctg gctgcgttta tcattcccca
    39541 aaaccttaac atggctatgg tttatggcaa ttcatcataa aaatcagcat tatgcatgtc
    39601 attgatttgt cttattttgt aaaaatatat cgttcaaaaa ataagcacat ctctgtcaat
    39661 tttttgcgtc atttgcatcg cgcaggcgac ctacacttat caggcactac ccgttgtatt
    39721 tatgaggatg tcattatgaa aaagtgcctc acactactga ttgccaccgt cctgagcgga
    39781 atctctctca cggcttatgc cgcgcaaccg atgagtaacc tggacagcgg tcagctacgg
    39841 cccgccggta ccgtttcggc aactggcgca tcaaacctaa gcgatctgga ggataaactg
    39901 gcagaaaaag cgcgcgaaca aggcgcgaaa ggttatgtca ttaattccgc aggcggaaat
    39961 gaccagatgt tcggtactgc aaccatctac aaataacccc accaacgtaa ctgcattcgc
    40021 ctctggatgc agttttcgcc tgctttgtaa tcaaaaaaca tcaaatatga acgcaatgta
    40081 aataaatgta tttctttttc gcgcaatggg tgatagaaaa tcgcgtcaag tgataatgct
    40141 tatcaaaatt attatcactt gcgcgaacac tatcacggga ttaacagtgg catcgcatcc
    40201 gcagagatgc tttctcgtgg cagtgaaaat ttcaacatat aagaaaaagt cacctgcaaa
    40261 atggaaaaca atcgcaattt ccctgccaga caatttcatt cgctcacgtt ctttgccggt
    40321 ctttgtattg gtattacgcc tgtggcacag gcactcgccg ccgaagggca agctaacgcg
    40381 gatgacacgc tggttgtcga agcatcaacg ccttcgcttt atgcgccaca acaatctgcc
    40441 gatccgaaat tctcgcgtcc ggtagcggat actacccgca cgatgacggt gatttctgaa
    40501 caagtgatta aagatcaggg cgcaaccaac cttaccgacg cgctcaaaaa cgtccccggc
    40561 gtg
//
LOCUS       DBKEKI010000023        40176 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.023, whole genome shotgun sequence.
ACCESSION   DBKEKI010000023 DBKEKI010000000
VERSION     DBKEKI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.023"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..514)
                     /locus_tag="ACUQPX_001164"
                     /pseudo
     CDS             complement(1..514)
                     /locus_tag="ACUQPX_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(972..1058)
                     /locus_tag="ACUQPX_001165"
     tRNA            complement(972..1058)
                     /locus_tag="ACUQPX_001165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1022..1024),aa:Leu,seq:gag)
     gene            complement(1073..1405)
                     /gene="secG"
                     /locus_tag="ACUQPX_001166"
     CDS             complement(1073..1405)
                     /gene="secG"
                     /locus_tag="ACUQPX_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HHV9055931.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLTTLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1633..2970)
                     /gene="glmM"
                     /locus_tag="ACUQPX_001167"
     CDS             complement(1633..2970)
                     /gene="glmM"
                     /locus_tag="ACUQPX_001167"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HHV9055932.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2963..3811)
                     /gene="folP"
                     /locus_tag="ACUQPX_001168"
     CDS             complement(2963..3811)
                     /gene="folP"
                     /locus_tag="ACUQPX_001168"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HHV9055933.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3901..5835)
                     /gene="ftsH"
                     /locus_tag="ACUQPX_001169"
     CDS             complement(3901..5835)
                     /gene="ftsH"
                     /locus_tag="ACUQPX_001169"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HHV9055934.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMPEQLGDK"
     gene            complement(5935..6564)
                     /gene="rlmE"
                     /locus_tag="ACUQPX_001170"
     CDS             complement(5935..6564)
                     /gene="rlmE"
                     /locus_tag="ACUQPX_001170"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HHV9055935.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6690..6983
                     /gene="yhbY"
                     /locus_tag="ACUQPX_001171"
     CDS             6690..6983
                     /gene="yhbY"
                     /locus_tag="ACUQPX_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HHV9055936.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(7139..7615)
                     /gene="greA"
                     /locus_tag="ACUQPX_001172"
     CDS             complement(7139..7615)
                     /gene="greA"
                     /locus_tag="ACUQPX_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HHV9055937.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7863..9296
                     /gene="dacB"
                     /locus_tag="ACUQPX_001173"
     CDS             7863..9296
                     /gene="dacB"
                     /locus_tag="ACUQPX_001173"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HHV9055938.1"
                     /translation="MRFSRFIIGLTSCIAFRVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(9482..10642)
                     /gene="cgtA"
                     /locus_tag="ACUQPX_001174"
     CDS             complement(9482..10642)
                     /gene="cgtA"
                     /locus_tag="ACUQPX_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HHV9055939.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDGEDWDEDDEEGVEFIYKR"
     gene            complement(10658..11623)
                     /locus_tag="ACUQPX_001175"
     CDS             complement(10658..11623)
                     /locus_tag="ACUQPX_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HHV9055940.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11750..12007)
                     /gene="rpmA"
                     /locus_tag="ACUQPX_001176"
     CDS             complement(11750..12007)
                     /gene="rpmA"
                     /locus_tag="ACUQPX_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HHV9055941.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(12028..12339)
                     /gene="rplU"
                     /locus_tag="ACUQPX_001177"
     CDS             complement(12028..12339)
                     /gene="rplU"
                     /locus_tag="ACUQPX_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HHV9055942.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            12598..13569
                     /gene="ispB"
                     /locus_tag="ACUQPX_001178"
     CDS             12598..13569
                     /gene="ispB"
                     /locus_tag="ACUQPX_001178"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HHV9055943.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13798..14076
                     /gene="sfsB"
                     /locus_tag="ACUQPX_001179"
     CDS             13798..14076
                     /gene="sfsB"
                     /locus_tag="ACUQPX_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HHV9055944.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(14124..15383)
                     /gene="murA"
                     /locus_tag="ACUQPX_001180"
     CDS             complement(14124..15383)
                     /gene="murA"
                     /locus_tag="ACUQPX_001180"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HHV9055945.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(15438..15692)
                     /gene="ibaG"
                     /locus_tag="ACUQPX_001181"
     CDS             complement(15438..15692)
                     /gene="ibaG"
                     /locus_tag="ACUQPX_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HHV9055946.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15852..16145)
                     /gene="mlaB"
                     /locus_tag="ACUQPX_001182"
     CDS             complement(15852..16145)
                     /gene="mlaB"
                     /locus_tag="ACUQPX_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HHV9055947.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(16145..16780)
                     /gene="mlaC"
                     /locus_tag="ACUQPX_001183"
     CDS             complement(16145..16780)
                     /gene="mlaC"
                     /locus_tag="ACUQPX_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HHV9055948.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QVYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16799..17350)
                     /gene="mlaD"
                     /locus_tag="ACUQPX_001184"
     CDS             complement(16799..17350)
                     /gene="mlaD"
                     /locus_tag="ACUQPX_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HHV9055949.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(17355..18137)
                     /gene="mlaE"
                     /locus_tag="ACUQPX_001185"
     CDS             complement(17355..18137)
                     /gene="mlaE"
                     /locus_tag="ACUQPX_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HHV9055950.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(18145..18954)
                     /gene="mlaF"
                     /locus_tag="ACUQPX_001186"
     CDS             complement(18145..18954)
                     /gene="mlaF"
                     /locus_tag="ACUQPX_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HHV9055951.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            19164..20141
                     /locus_tag="ACUQPX_001187"
     CDS             19164..20141
                     /locus_tag="ACUQPX_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HHV9055952.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            20155..21141
                     /gene="kdsD"
                     /locus_tag="ACUQPX_001188"
     CDS             20155..21141
                     /gene="kdsD"
                     /locus_tag="ACUQPX_001188"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HHV9055953.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRNALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            21162..21728
                     /gene="kdsC"
                     /locus_tag="ACUQPX_001189"
     CDS             21162..21728
                     /gene="kdsC"
                     /locus_tag="ACUQPX_001189"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HHV9055954.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVGDAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21725..22300
                     /gene="lptC"
                     /locus_tag="ACUQPX_001190"
     CDS             21725..22300
                     /gene="lptC"
                     /locus_tag="ACUQPX_001190"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HHV9055955.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            22269..22826
                     /gene="lptA"
                     /locus_tag="ACUQPX_001191"
     CDS             22269..22826
                     /gene="lptA"
                     /locus_tag="ACUQPX_001191"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HHV9055956.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22833..23558
                     /gene="lptB"
                     /locus_tag="ACUQPX_001192"
     CDS             22833..23558
                     /gene="lptB"
                     /locus_tag="ACUQPX_001192"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HHV9055957.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            23606..25039
                     /gene="rpoN"
                     /locus_tag="ACUQPX_001193"
     CDS             23606..25039
                     /gene="rpoN"
                     /locus_tag="ACUQPX_001193"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HHV9055958.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            25062..25349
                     /gene="hpf"
                     /locus_tag="ACUQPX_001194"
     CDS             25062..25349
                     /gene="hpf"
                     /locus_tag="ACUQPX_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HHV9055959.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            25467..25958
                     /gene="ptsN"
                     /locus_tag="ACUQPX_001195"
     CDS             25467..25958
                     /gene="ptsN"
                     /locus_tag="ACUQPX_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HHV9055960.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            26004..26858
                     /gene="rapZ"
                     /locus_tag="ACUQPX_001196"
     CDS             26004..26858
                     /gene="rapZ"
                     /locus_tag="ACUQPX_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HHV9055961.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26855..27127
                     /gene="npr"
                     /locus_tag="ACUQPX_001197"
     CDS             26855..27127
                     /gene="npr"
                     /locus_tag="ACUQPX_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HHV9055962.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            27340..27972
                     /gene="yrbL"
                     /locus_tag="ACUQPX_001198"
     CDS             27340..27972
                     /gene="yrbL"
                     /locus_tag="ACUQPX_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HHV9055963.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27969..28697)
                     /gene="mtgA"
                     /locus_tag="ACUQPX_001199"
     CDS             complement(27969..28697)
                     /gene="mtgA"
                     /locus_tag="ACUQPX_001199"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HHV9055964.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGSEPFMQQHQLD"
     gene            complement(28694..29347)
                     /gene="elbB"
                     /locus_tag="ACUQPX_001200"
     CDS             complement(28694..29347)
                     /gene="elbB"
                     /locus_tag="ACUQPX_001200"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HHV9055965.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKELAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(29577..31913)
                     /gene="arcB"
                     /locus_tag="ACUQPX_001201"
     CDS             complement(29577..31913)
                     /gene="arcB"
                     /locus_tag="ACUQPX_001201"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HHV9055966.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKNNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(32009..32938)
                     /locus_tag="ACUQPX_001202"
     CDS             complement(32009..32938)
                     /locus_tag="ACUQPX_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HHV9055967.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGIRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            33613..38073
                     /gene="gltB"
                     /locus_tag="ACUQPX_001203"
     CDS             33613..38073
                     /gene="gltB"
                     /locus_tag="ACUQPX_001203"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HHV9055968.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDMTFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            38086..39504
                     /gene="gltD"
                     /locus_tag="ACUQPX_001204"
     CDS             38086..39504
                     /gene="gltD"
                     /locus_tag="ACUQPX_001204"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HHV9055969.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            39688..40092
                     /locus_tag="ACUQPX_001205"
                     /pseudo
     CDS             39688..40092
                     /locus_tag="ACUQPX_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
BASE COUNT         9961 a        10843 c         9919 g         9453 t
ORIGIN      
        1 cgctttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga
       61 aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt
      121 tattattact gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat aagggatata
      181 catccccagc attttcacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc
      241 atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc
      301 agtcagtaac acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt
      361 aacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg caccggaaga
      421 ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt
      481 aaaatcttct agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata
      541 taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata
      601 tcagaccgaa acactgttag tttttctttt taaatgatgc gaccattgcg ttctcagtaa
      661 ttaaataaca ttatcaatgc gtctctaaac attacgcaga acgtagccat cagtagttat
      721 actgacagga gcaggatggt ttatcgagaa gtaaacatat ccgtattcgt accggaactg
      781 gagttgacac aataaagtgc caattatgtc agcagtaggg aaaagtttat aactaaggcg
      841 taccggcacc atcgtttcaa ggtaccagct acgagtaaag caactggacg agatacagat
      901 acctgacaac cattcatcag accaggacca aaacgaaaag agacgctttt cagcgtctct
      961 tttctggaat ttggtaccga ggacgggact tgaacccgta agccctattg ggcactacca
     1021 cctcaaggta gcgtgtctac caattccacc acctcggcac ggatactact ttttagttcg
     1081 ggatatcgct ggtcggctta gccggagcag ctggctgagt ttgttcggtt ttcgccggtg
     1141 cactcagatt ttcccattcg ctacctctat tggttttgtt gctattgatg ttacccagca
     1201 ccagactgat gatgaagaat aacgttgtca gcagcgccgt catgcgggtc atgaagttac
     1261 cagaaccact tgaaccaaac agcgtagcgg aagcgcctgc tccgaaggag gctcccatat
     1321 cagcgccttt accttgctgc agcatgatca gaccaacaag gccaattgcc acaataagga
     1381 aaactactaa aagagcttcg tacataatca acctgttcct tgcggagttg ccgcgtacca
     1441 atgcttcaac caataaagcg ggagttttta atctttccca ctgaagcggg tgtgaatact
     1501 aaccaaagcg aatgaccttc gcaagggcaa ttttatcgca ttgtatcaac tgcggaaaaa
     1561 aacagcaaaa ccgatgtgtt cgccgagaaa aaaggcgaca atcgccgcct ttttagccag
     1621 ttatctaacg ctttaaacgg cttttactgc atcggcgatg cggtgtgcaa attcagtcac
     1681 ctgcgcttcg tcttcgcctt ccaccatcac gcgaattaac ggttcggtgc cggatttacg
     1741 cagcaacacg cgtccacggt tgcccagcgc agcttcaacc tctgcggtca cggctttaac
     1801 tgactcatgc tcaagcggat cgccgctacc tgcggtgtaa cgtacgttaa ccagaatctg
     1861 cgggaacatt ttcatgccgc tgcaaaggtc gtgcaggctc atatggttac gtgccatcgc
     1921 cgccagcacc tgcaagccag caacgatgcc gtcaccggta gtggttttat ccagcaggat
     1981 cacatgaccg gaattctctg caccgatacg ccagcctttc tcctgcattt tttccagtac
     2041 gtagcggtca cccactttcg cgcgcgcaaa tggaattcct aactgtttca gcgccagttc
     2101 aagccccatg ttgctcatca atgtacccac agcgccacca cgcagctggc cctgacgaag
     2161 accttcacgc gcgatgatat acatgatctg atcgccatcg actttattgc cttcatggtc
     2221 aaccataatc acgcgatcgc cgtcgccgtc gaaggcaata ccgagatccg ctttttcagc
     2281 cagcacacga gcctggagcg cgcgaacgtc ggtagccccc acttcggcat tgatgtttac
     2341 accgtttggc tcacaaccga tagcgataac gttcgccccc agttcgcgca gcacgttcgg
     2401 cgcgatgtga taagtcgcac cgtttgcaca atccaccaca atcttcagtt cactgaggct
     2461 aagttcgttc gggaacgtgg ctttgcaaaa ctcgatatag cgacccgcgg catcaacgat
     2521 acggctggct ttacccagtt ctgccgaatc aacgcagctg atctcctttt ccatttccgc
     2581 ttcgatggcc tcttctaccg catccggcag tttagtgccg tcgatagaga agaatttaat
     2641 cccattatcg tagaacgggt tatgcgatgc agatatcaca attccggcct ctgcgcggaa
     2701 ggtacgcgtc agataagcca cggccggtgt tggcatcggg ccagtgaaga gtgcggaaag
     2761 gcccgctgcc gccagacccg cttccagtgc tgactccagc atatagccag aaatacgcgt
     2821 gtctttacca ataataatct tacgggagcc gtggcgcgcc agcactttac ccgcggccca
     2881 acccagctta agcacaaaat caggtgtgat cggcgcatcc cctacacgac cacgaatccc
     2941 atcggtaccg aaatatttac gattactcat agcgtttgtt ttcctttgca gacagagtgg
     3001 cttccaccac ccgcatcgct tctacggttt ctttgacgtc atgaacacga atgatgtgcg
     3061 cgccttgcat tgcggcaatg accgcacagg ccagactacc gctcaggcgc tcggacggcc
     3121 ccacgttcag cagctgccca atcatcgatt ttcgtgacat acccaccaac agcggcaggt
     3181 tgaaatggtg aaattcagcc aggcgcgcca gtaatgaata gttatgggag agatttttac
     3241 cgaaaccgaa tccggggtcg agcaacaatt tctcttttgc gatacccgcc tgctcgcaac
     3301 gtgctatttg ctcaataaag tagcgattca cttctgcaaa gacatcgtca tacttcggag
     3361 cttcctgcat ggtttttgga tttccctgca tatgcatcag acaaaccggt aaaccggttt
     3421 ctgcagccgc ctccagagcg ccaggttcgg aaagggagcg gatatcatta ataatgtgag
     3481 cgccaacttt cgctgactca cggatgactt ctggtttgga tgtatcgacc gagatccaga
     3541 cttcgaagcg ttgagcaatt gcctcaacca caggaataac acgttgcaac tcttcttcaa
     3601 cgctaacttc cgccgcccct gggcgcgtgg actcgccacc aacgtcaatg atcgtcgcgc
     3661 cagcattgat catcagattc gcatgtttca ccgcatctat cagcgagtta tgcgtgccac
     3721 catccgaaaa ggaatcaggc gtgacgttga ggatccccat tacgtgagga tggctaaggt
     3781 ccagtgaagt accctgggca aagagtttca tgatgttatc cctggtatga attgataaga
     3841 aaaaaccccg gagcacgccc cggggttttc ggtacaaata cagtcatctg atgcgggaac
     3901 ttacttgtcg cctaactgct ctggcatggt gttacccggg ttcggcgtac gcggttcatc
     3961 aaccggacga ggagcctttg gactaccatt gtcgccagaa ttgttagaag cgcctggttc
     4021 ttcccagccc gctggcggac gtacatcgcg acgtgccatc aggtcatcaa tctgcggtgc
     4081 gtcgatagtc tcatatttca tgagagcatc tttcatcgca tgcagaatat ccatattgtc
     4141 ggtcagaagc tgacgcgcac gattatagtt acgctcaatc agtgctttca cttcctggtc
     4201 gatgatacgt gcagtttcat cggacatatg tttcgctttc gctacgctac ggccgaggaa
     4261 tacttcacct tcttcttccg cgtacagcag tggacccaat ttctcagaga agccccactg
     4321 agtcaccatg ttacgtgcca ggttggtcgc aactttaata tcgttggacg caccggtaga
     4381 aacatgttcc ggcccgtaga tgatctcttc tgccagacga ccaccgtaca gcgtagaaat
     4441 ctggctttcc agtttctgac ggctggcgct gattgcgtcg ccctcaggca agaagaaagt
     4501 cacacccagc gcacgaccgc gtgggataat cgtcacttta tgcaccggat cgtgttccgg
     4561 caccaggcga ccgataatcg catgacccgc ttcgtggtaa gccgttgatt ctttctgcgc
     4621 ttccgtcatc accatggagc gacgttccgc acccatcatg attttgtctt tcgctttctc
     4681 gaactcaacc atcgacacaa cgcgtttgtt gccacgagca gcgaacagtg ccgcttcgtt
     4741 caccaggttc gctaggtcag caccggagaa accaggagta ccacgggcaa tgattgccgc
     4801 gtcgatatcg ggtgccaatg gtacgcgacg catgtgaact ttcaggatct gctcacgacc
     4861 gcgaacatct ggcaagccga ccacaacctg gcggtcgaaa cggccaggac gcagcagggc
     4921 cgggtcgaga acgtccggac ggttagtcgc ggcgataacg atgatacctt cgttaccttc
     4981 gaagccatcc atctcaacca gcatctggtt cagagtctgt tcacgttcat cgtgaccacc
     5041 gcccagacca gcgccacgct ggcggcctac ggcgtcgatt tcatcgataa agatgatgca
     5101 cggtgccgct ttcttcgcct gttcgaacat gtcacgaaca cgggatgcac ccacaccgac
     5161 gaacatttct acgaagtcag aaccggagat agtaaagaac ggaactttcg cttcgcctgc
     5221 aatcgctttc gccagcaacg ttttaccggt acccggagga ccgaccatca ggacgccttt
     5281 cgggatctta ccgccgagtt tctggaagcg gctcggctcg cgcagatact ctaccagttc
     5341 agcaacttct tcttttgctt cgtcgcagcc cgcaacgtca gcaaaggtcg ttttgatctg
     5401 atcttctgtc agcatgcgcg ctttgctctt accaaacgac atggcacctt tgccaccgcc
     5461 gccctgcatt tgacgcatga agaagatcca gacaccaatc agcaacagca tcgggaacca
     5521 ggagatgaag atagaagcca gcaggcttgg ttcttcaggc ggttcaccga caaccttgac
     5581 gttcttggtc aacaggttat ccagtaattt cggatcctga accggaatgt aagtggtata
     5641 acggttacta tctttcttgg taacgttgat ttcacgtccg ttgatacgcg cttcacgaac
     5701 ctggtcgtta ttgacctctt gtaggaaggt agagtaatcc accttacggc cattagactc
     5761 gctgggccca aagctctgga atactgacat cagcacaacg gcaatgacca gccagagtat
     5821 taggtttttc gccatgtcac tcaagggatt aacctcttat tacaactgtg ttaaaaacag
     5881 cgtcaggata ctctatatcc agcatctttc aaactttcgt ctgaaatctc ccggttaggg
     5941 tttacgcccg gtcgctacaa tatacacttc tcgcgaacgt gcacgagaag agtccggctt
     6001 acgaactttg accttcgtaa acagggagcg aatttccctt agatactcat cgaaaccttc
     6061 gccctggaac accttcacta caaaactgcc acctggcgct aatacatcac gacacatttc
     6121 tagcgccagt tccaccagat acatggcacg ggggatatcc accgccggtg ttccgctcat
     6181 gtttggtgcc atatcagaca tgacaacctg gactttgctg tcgccaacgc gctccagcag
     6241 tgctttcatc accagttcat cacgaaaatc gccctgaaga aagtccacac caacgatagg
     6301 atccataggt aaaagatcgc aagcgatgat gcggcctttg ccgccaattt gggtgaccac
     6361 atattgtgac caaccacccg gagcagcacc aaggtcgaca accgtcattc ccggtttaaa
     6421 gagtttgtca ctttgctgta tttcatcaag tttaaaccaa gcacgggaac gtaatccctt
     6481 tttctgtgcc tgttgaacat atttatcgct aaagtgttcc tgaagccagc ggctggagct
     6541 ggcagaacgc ttcttacctg tcatttaact ttcccatggg gataactcat cgtaaccaat
     6601 tgcgtaaatt tttacgcgcc tatttggtga tatatgggag atggcggtag aatgacccgt
     6661 tttcaatccc aacgtaagca aaaaatacga tgaatctgag tactaaacaa aaacagcacc
     6721 tgaaaggtct ggcacatccg ctcaagccag ttgttctgct tggcagtaat ggtttgaccg
     6781 aaggggtgct ggccgagatt gaacaagcgt tagagcacca tgaactcatc aaggtgaaaa
     6841 tcgccaccga agatcgcgaa acaaaaacct tgatcgtgga agctatcgtg cgcgaaaccg
     6901 gcgcctgtaa tgtacaggtc atcggtaaaa cgctggtgct ttatcgccca actaaagaac
     6961 gtaaaatctc gctgccacgc taagattatc ctaaagttac acacatttgc tgtgtaaaac
     7021 gaggggtttt ccgcaggcag gagagcaaaa tgccacgctc tgttcgttga taaaaggccg
     7081 cagagcggcc tttttccttt ctttacaata catcaacatc ttgagtattg ggtaattctt
     7141 acaggtattc caccttaatt acttcaaatt ctacttcgcc gcccggcgtt ttgatgacca
     7201 caacatcatc ttcttctttg ccgatcaggc cacgagcaat aggcgagttt acagaaatca
     7261 ggttttgttt aaagtcagct tcgtcatcgc caacgatgcg ataagtctgt tcttcgtcag
     7321 aatccagatt cagcaccgtt acggtagcac caaaaataac gcgcccattg ttgggcattt
     7381 tggtgacatc aatcacctgc gcgtttgaca gcttggcttc gatgtctttg atacggcctt
     7441 cgcagaagcc ctgctgttca cgagctgcgt ggtattcggc gttttctttc aggtcgccat
     7501 gctcacgcgc ttccgcgata gcagcaatga tttcaggacg gcgcacagat ttcagaaaat
     7561 ccagctcttc gcgtaatttt tcagcgccgc gtaaggtcat cggaatagct tgcatttgtt
     7621 atacctcttg aatattcctg atggggcaag tcttcaccca ccccggctgt tcggcctgcc
     7681 cggcataact cctcaccggg accagaagca aaaaaatacc gacccgggta caagtcccag
     7741 gtcagctaca attcacattt tgatggtcat tttaccctga agttcccgaa gggtcatcgt
     7801 ttactttata gggcgttgcg ccgtagtatg acggctcgat tccaggttgt tagcgcgaga
     7861 ttatgcgatt ttccagattt atcatcggat tgaccagctg tatagcgttc agggttcagg
     7921 ccgcgaatgt tgatgagtac gttactcaac tccccgcagg tgccaacctt gccctgatgg
     7981 tgcaaaaagt cggtgcgtcg gcccccgcta ttgattacca cagtcagcag atggcgctgc
     8041 ccgccagtac ccagaaagtg attactgcgc tggcggcgtt gattcaactc ggccccgatt
     8101 ttcgtttcac cacgacgctt gaaaccaaag gcaatgtgga aaacggcgta cttaagggtg
     8161 acttagtggc gcgatttggt gccgatccga cgttaaaacg tcaggatatt cgcaatatgg
     8221 tcgcgacttt gaaaaaatct ggcgtcaacc aaatcgatgg caatgtgttg atagatacct
     8281 ccattttcgc cagccacgat aaagcccccg gctggccatg gaatgacatg acacaatgct
     8341 ttagcgctcc gcctgctgcc gccatagttg accgcaactg tttctccgtc tcgctctaca
     8401 gtgccccaaa gcctggtgat atggctttta tacgcgtggc atcttattac cccgttacga
     8461 tgttcagcca ggtacgcacc ctcccccgtg gttctgccga agcgcaatac tgcgaactgg
     8521 atgtggtgcc aggcgacctg aaccgcttta cgctgacggg atgcctgcca caacgttctg
     8581 agccgctccc gttggctttt gccgtgcagg atggagccag ctatgccggt gcaattctga
     8641 aagatgagtt aaaacaggcg ggtatcacct ggagcggaac actgctgcgc cagactcagg
     8701 ttaacgaacc tggaacggta gttgccagta aacagtcggc cccgctgcac gatctgctca
     8761 agattatgct gaaaaagtcg gacaacatga tcgccgatac ggttttccgc atgatcggcc
     8821 atgcgcgctt caatgtgcct ggaacatggc gggctgggtc ggacgccgtg cgtcagatcc
     8881 tgcgccagca agccggtgtc gatattggta acaccattat tgccgatggt tcagggcttt
     8941 cgcggcataa cctgattgcc cccgccacca tgatgcaggt gctgcaatac attgcccaac
     9001 acgacaatga acttaacttt atctccatgc tgccgcttgc gggctatgac ggctctttgc
     9061 agtaccgtgc aggtctgcat caggcgggcg tggatggcaa agtctcagcg aaaaccggtt
     9121 cgttgcaggg ggtatataac ctggcgggat tcattaccac agcgagcggg caacgaatgg
     9181 catttgtaca atatctttct ggctatgcag tagaacccgc ggatcagcgt aatcgccgta
     9241 ttccgttagt gcgttttgaa agccgtttgt acaaagatat ttatcagaac aattagtcaa
     9301 aaagaaaccc cggcgcatgg ccggggtttc agattattga caaagtgcgc tttgtttatg
     9361 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaatcg tgcaaattca atatattgca
     9421 atcatcttgt aggcctgata agtgtagcgc atcaggctta tttggcattg gtcattagtg
     9481 attaacgctt gtaaatgaac tcaacgcctt cttcgtcgtc ttcgtcccag tcttcaccat
     9541 cctcttcagc aatctcttca agctgctgac ggtgataatc atcccacatg aattcgactt
     9601 tctctggctg tttcgcttct tcagcctgca cgaccgggtt ttcaatgata aaggtcatca
     9661 catcccagca gagatctttc acgcccagtc cgctcgccgc agagatcaga taatatttat
     9721 cttcccagcc cagcgcttca gcgatcgctt tcgctttctc ttcggcttct gccttatcca
     9781 gcagatcgat cttgttgaag actaaccaac gcggtttcgc cgccagatcc tggctgtatt
     9841 tttccagctc gctgataata atacgcgcgt tttcaaccgg atcggtgccg tcaatcggat
     9901 cgatatcgat gaggtgcaac aggacgcggc aacgttccag gtgcttcagg aagcgaatgc
     9961 ccagacctgc gccttccgca gcgccttcaa tcagtcctgg aatatcggca acaacgaagc
    10021 tcttttcgtt gtccattcgt accacaccca gacttggcac cagagtggta aacggataat
    10081 ccgccacttt cggtttagcc gccgataccg cacgaataaa ggtcgattta cccgcgtttg
    10141 gcatccccaa cataccgacg tcagccagca gcatcagctc cagcagcagc tcacgcttat
    10201 cgcccggcgt accgttagtt ttctgccgtg gtgtacggtt aactgacgat ttgaaacggg
    10261 tattgcccag accgtgccag ccgcccttag caaccagcag acgctgaccg tgtttagtca
    10321 tatcgcccat ggtttcacca gtaccctggt cgattacacg cgtacctacc ggcactttaa
    10381 tcgtcacgtc tttaccgcgc ttaccggtac aatcgcggct tgcgccgttc tgaccgcgct
    10441 ctgcacggaa agatttttca aaacgataat cgataagcgt gttcaggttc tcgtcggctt
    10501 ccatccatac gtcaccacca tcaccgccgt cgccgccatc cgggccgcct ttcggaatat
    10561 acttttcgcg gcggaagctc acgcaaccat taccgccatc acctgcaacg accagaatcg
    10621 atgcttcatc aacaaacttc attttattct ccgtaaatca ttcgcctgcg cggggttgcg
    10681 ataccaccgt tgtatgctta cgtaatccgc cccaaatacg atgaccaatg gcggaataca
    10741 tcgcgcccgc aaccacgaca aacgcaccga gataacctaa aaggtttaac atcggtctgg
    10801 cgaagaaatc gggccaggcc agtgataaaa gatctgaaaa aaacagcgta aacagtgggg
    10861 tgagcgtgat gatcgcgctc acctgcgctg cctgccagcg agccatcgct tccgccaggg
    10921 cgccatatcc taccaaggta ttcagtccgc aaaaaattaa acatgcgagc tgccagtggc
    10981 taagctgcgc tatcactcca ggctttgcca gagggaagag cgcgattgta cataaagtgt
    11041 acagtaaaaa caggatctgc ggtgaggcca gccgacgcaa taaaaccttt tgcgccacgc
    11101 cataactcac ccaaaccgtc gccgcaccga ccccaaagat aactccccag gtgtaatcgg
    11161 tgagctttgt aaatatctcg accagactgg tgttaaaaaa catcaccaga ccgctcagga
    11221 gcatcaatgc ccctacaacc tgagtgctgc gcattttctc tttcaggata aatacgctgg
    11281 caaccatcat gccaactggc gagagttgcc caatcacctg cgaagcggtc ggactcaggt
    11341 attgcaagga tgagctgaac aggatgaagt tcccaaacag cccggcggtc gccactgcca
    11401 acaaaatcaa ccagcgtggc ttacgaaaca cgcgtaatgg cggcaacctc ttcttcaccg
    11461 caagaatggc accaaggcca atactcgcca tcaagaaacg gtaaaacacg attgtcggag
    11521 gttccatcac ctccagcacc tgcttcattg cgattggcaa cgccccccag caaattgctg
    11581 tggtaagcgc caaaagaatg ccaatgcctg cctgctgctt cataccgtat tccctgcaaa
    11641 gcgcatttgc ggattaacgt cgcaccatgc gcatttttac cggttatcga atgtaaaaag
    11701 ccccgcaacg tgttgcgggg ctttcatccg ttaccgggac gcgaaaaact tattcagctt
    11761 cgatgctgat aaatttacgg tttttcgggc ctttaacttc gaatttcact ttaccgtctg
    11821 ctttagcaaa cagagtgtgg tcacgaccgc aacctacgtt agcgccagcg tggaatttgg
    11881 taccacgttg acgaacgatg atgctacccg ccagaacgga ttcgccaccg aaacgcttaa
    11941 cgcccaggcg tttagcttct gaatcgcgac cgttacgtgt ggagccgcca gcctttttat
    12001 gtgccatttg aaatctctcc tcaggtctta ggcgctgatg ccagtaattt tcacatcagt
    12061 gaaccactga cgatggccct gctgcttacg atagtgttta cgacgacgaa acttaacgat
    12121 tttaactttc tcgccacgac cgtgagcaac aacttcagct ttgattacgc cgccatcaac
    12181 gaaaggaacg ccgattttga cttcttcacc gtttgcgatc atcagcactt cagcgaactc
    12241 aacagtttcg ccagttgcga tgtccagctt ttccaggcga acggtctgac cttcgcttac
    12301 tcggtgttgt ttaccaccac tttggaaaac cgcgtacata taaaactccg cttctgcgca
    12361 caccttttca atgattcaga gtgcgctata aatattcaca atagggcgcg aatattacgc
    12421 aaaacgcacg cctttgacaa gtgctacagt caatacacga agaaaaaaaa cacaacttgt
    12481 acggtaacgt ttatctgtgc cattttttca gtacaattac cctatattcc taaccataaa
    12541 ccctaagttg cctttgttca cagtaaggta atcggggcga aaagcccggc ttttgcgatg
    12601 aatttagaaa aaatcaatga gttaaccgcg caagatatgg cgggtgttaa tgcggcaatc
    12661 cttgagcagc ttaattccga cgtccaactg atcaatcagt taggctatta catcgtcagc
    12721 ggcggcggta aacgtattcg tccgatgatt gctgtactgg ctgcacgagc tgttggctat
    12781 gagggaaatg cgcatgtcac cattgctgcc ctgatcgagt ttatccacac ggcgactctg
    12841 ctacacgacg acgttgtgga tgaatcagat atgcgcaggg gtaaagctac cgccaacgcc
    12901 gcatttggca atgccgccag cgtgctggta ggcgatttta tttatacccg cgcgttccag
    12961 atgatgacca gcctcggttc actcaaagtg ctggaagtca tgtcagaagc tgtaaacgtc
    13021 atcgcagaag gtgaagttct gcaactgatg aacgttaacg atccggacat cactgaagaa
    13081 aactacatgc gcgttatcta tagcaaaacc gcgcgtctgt ttgaggctgc cgcgcagtgt
    13141 tccgggattc tggctggctg tacgccggag gaggagaaag gcctgcagga ttatgggcgc
    13201 tatctcggca ctgctttcca gttgatcgac gatttactcg attacaatgc cgatggcgaa
    13261 cagttaggta aaaacgtcgg cgacgatctg aacgaaggta aaccgacgct gccgctgctg
    13321 catgcgatgc atcatggcac accagaacag gcacagatga tccgtaccgc catcgaacag
    13381 ggtaacggtc gccatcttct ggaaccggtt ctggaagcaa tgaacgcttg tggatctctt
    13441 gaatggacgc gtcagcgcgc cgaggaagaa gcagacaaag ccatcgcagc gttacaggtg
    13501 ctcccggaca ccccttggcg agaagcactc atcggcctcg cgcacatcgc tgttcaacgc
    13561 gatcgttaat cccctccctt catcccgcgc agtgcgcggg atgattccag taaattccat
    13621 aaaaacacta attcagcgct ataacacctg aaaaaaaagg gcgatgaatc tttatatatt
    13681 ctgaatattc acactcttta caggaacttt ttagcgcaat aggccatcag gagtatagtg
    13741 atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg aggaaagatg
    13801 gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg caagaaagga
    13861 acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct ggcaaacgca
    13921 ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg aactgacccc
    13981 tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat cgacagaacg
    14041 cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta gccccgcgaa
    14101 cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt tgcacctaaa
    14161 gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat acgatcaacc
    14221 accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc acgcagatcg
    14281 gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac ggtattgctt
    14341 tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa gcggttttca
    14401 aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag cgtgaactgg
    14461 gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac agccttcgga
    14521 cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc cgctccagca
    14581 tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg gcagataatt
    14641 ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg atccggcagc
    14701 acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg atcggtgccc
    14761 tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac gatttccggt
    14821 tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc acacatgatg
    14881 gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt cagacgacca
    14941 tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa ttgttcgagg
    15001 ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg tagtgaaact
    15061 tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc acgcatggtt
    15121 ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat atgcacagaa
    15181 ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt atcgacgtct
    15241 ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc ggcaaaaaga
    15301 ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag cttcgttggc
    15361 ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat atccgctacc
    15421 ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca ctccgcaggg
    15481 gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc catcagcggg
    15541 ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc acccacggca
    15601 ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga gagagcgttc
    15661 atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag ttaataaacc
    15721 agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc tcgctgataa
    15781 atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg ccatatccgg
    15841 cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt cgcgagggta
    15901 tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt cgccagatcg
    15961 ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg gctaagatcg
    16021 atgcaggtaa tccccttcac cgcttcctca cgcatttccc aaagcggtag cagaacgtcc
    16081 tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct cagtgactcg
    16141 ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc agttgcgcag
    16201 tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt ttggtggtga
    16261 tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc tgggagtttt
    16321 tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata atggtaacgc
    16381 gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga taggtttgac
    16441 cgtgatacat cgctagcgcc tgaccgtaaa cctgcttcag gtactcacgg aaagcggcaa
    16501 agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc accagcgcac
    16561 cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta cgcagataat
    16621 ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt ttctgcgccg
    16681 cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca ctcagaggtg
    16741 caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc tcctgaaatt
    16801 atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca gctggcgcat
    16861 cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca atgagatctt
    16921 ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg atagcagtcc
    16981 ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc agcaggccgg
    17041 aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt tcaatttcca
    17101 gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc cggcccacaa
    17161 caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta tcgaacgtcg
    17221 cataaagcgt gtaggtcggt tcagtacgta tggacgtcac gttcgccgcc ttcaggcaaa
    17281 caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt tcattttttt
    17341 tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc cagccccaga
    17401 acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc ggcagacgtc
    17461 gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc gaacaccacg
    17521 ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg cattgccgac
    17581 cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc gccccagatc
    17641 cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc ccagaaacgg
    17701 ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga gagttgctct
    17761 gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc ggcaaacaac
    17821 agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag catacccaga
    17881 ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa caccattccg
    17941 atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt atagagctgg
    18001 cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc attgaataac
    18061 attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc gagcgacgcc
    18121 agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt gataatcgcc
    18181 ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact gacgtacgcg
    18241 cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt ttttgtccgc
    18301 caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag aaaccaccac
    18361 acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta cgcccatggt
    18421 gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct ccagcgcaat
    18481 cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag gcattagttt
    18541 agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat gcaacaatgg
    18601 cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata cgttcatatc
    18661 agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg tatacaggcg
    18721 cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat gatctggtgc
    18781 gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg gccccatgat
    18841 cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc agcgattgcc
    18901 acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct ccataattca
    18961 cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga acccaacata
    19021 tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac tttttagccg
    19081 cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg gaccggcgag
    19141 tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt gttggtttac
    19201 ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt tgccgaacct
    19261 ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca tcgttaccag
    19321 aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc gttggtacag
    19381 ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg ctggttcgtc
    19441 cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg ttggtcagcg
    19501 tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt atctttctcc
    19561 tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag gctgaacgtc
    19621 aggggactga cagcctgacc agagagcagc ttgcagagct gccgcgtgac ggcggattgc
    19681 ccgtcgcgtt tttatggctc ggcattgcgc ttatcatcat gccagtggcc acgcggatgg
    19741 tggttgataa cgccacggtg ctggcgaatt actttgccat cagcgagttg acgatgggtc
    19801 tgacggcaat tgctatcgga accagtctgc cggaactggc aaccgcaata gcgggggttc
    19861 gcaaaggtga aaacgacatt gctgtcggaa atatcattgg cgcaaacatt tttaatattg
    19921 tcatcgtgtt gggtttaccc gcgctgataa cgccaggaga gattgatcca ctggcgtaca
    19981 gtcgtgacta cagcgtgatg ttgctggtga gcattatttt tgcgttgctg tgctggcggc
    20041 gctccccgca accgggccgt ggtgtagggg tattattaac tggcggattt atcgtatggc
    20101 tggcgatgtt gtactggtta tcgccaatac tcgttgaata actggaaacg cattatgtcg
    20161 cacgtagagt tacaaccggg ttttgacttt cagcaagcag gtaaagaagt cctggcgatt
    20221 gaacgtgaat gcctggcgga gcttgatcaa tacatcaatc agaatttcac gcttgcctgt
    20281 gaaaagatgt tctggtgtaa agggaaagtt gtcgtcatgg ggatggggaa atcggggcac
    20341 atcgggcgca aaatggccgc aacgtttgcc agcaccggta caccttcatt tttcgtccat
    20401 cctggtgaag ccgcgcatgg tgatttaggc atggtcaccc cacaggatgt ggtgattgct
    20461 atctctaact caggtgaatc cagcgaaatc acggccttaa ttccagtgct taagcgtctt
    20521 cacgtaccgt taatctgcat caccggtcgc ccggagagca gcatggcgcg cgccgcagat
    20581 gtgcatctgt gtgttaaagt agcgaaagaa gcctgtccgt tagggctggc accgaccagc
    20641 agcaccaccg ccacgctggt tatgggcgat gccctcgctg tcgcgctgtt aaaagcacgc
    20701 ggctttactg ctgaagattt tgcgctctca cacccaggcg gcgcactggg tcgtaaactt
    20761 ctgctgcgcg taaacgatat tatgcatacg ggcgatgaga tcccgcatgt taagaaaacg
    20821 gccagtctgc gtaacgcatt gctggaagtt acccgcaaaa atcttggtat gactgtcatt
    20881 tgcgatgaca atatgatgat tgaaggcatc tttaccgacg gtgatttacg ccgtgtcttc
    20941 gatatgggcg tggatgttcg tcagttaagt attgccgatg tgatgacgcc ggggggaata
    21001 cgtgtgcgcc ctggcattct ggccgttgag gcactgaact taatgcagtc ccgccatatc
    21061 acctccgtga tggttgccga tggcgaccat ttactcggtg tgttacatat gcatgattta
    21121 ctgcgtgcag gcgtagtgta aagattcaag gataaacaac aatgagcaaa gcaggtgcgt
    21181 cgcttgcgac ctgttacggc cctgtcagcg ccgacgttat ggcaaaagca gagaacattc
    21241 gtctgctgat cctcgacgtt gatggcgtac tgtcagatgg cctgatttat atgggcaata
    21301 acggcgaaga gctgaaagcg ttcaatgttc gtgacggtta tggcattcgt tgtgcgctca
    21361 cctctgatat tgaagtcgct atcattaccg ggcgaaaggc taaactggta gaagatcgtt
    21421 gtgccacatt ggggatcact cacttgtatc aggggcagtc aaacaaactg atcgccttta
    21481 gcgatttgct ggaaaaattg gcgattgccc cggaaaatgt ggcttatgtt ggcgatgatc
    21541 tcatcgactg gccggtaatg gaaaaagtgg gtttaagcgt cgccgtgggc gatgcgcatc
    21601 cactgttgat cccgcgcgcc gattacgtga cgcgcattgc tggcggtcgt ggcgcagtgc
    21661 gcgaagtttg cgacttatta ctcctggcgc agggcaaact ggatgaagcc aaagggcaat
    21721 cgatatgagt aaagccagac gttgggttat cattgtgcta tcactagcgg ttctggtgat
    21781 gatcggcatt aatatggccg aaaaagacga taccgcccag gtggtcgtca acaacaatga
    21841 tcccacctat aaaagcgagc atacggacac gctcgtctat aacccagaag gggcactaag
    21901 ctatcgattg attgctcaac acgttgaata ttattccgat caggccgttt cgtggtttac
    21961 gcagccggta cttaccacgt ttgataagga taaaatcccg acatggtccg taaaagcaga
    22021 taaagccaag ctgaccaatg accggatgct ctatttatat ggacacgttg aagtcaacgc
    22081 acttgtgcca gactctcaac ttcgcagaat cacgacggat aacgcgcaga tcaatctggt
    22141 gacacaggat gttacctctg aagacctcgt cacgttatac ggaacaacat ttaactccag
    22201 cggtctgaaa atgcgcggca acttacgcag caagaacgcc gagctgattg aaaaggttag
    22261 aacatcctat gaaattcaaa acaaacaaac tcagccttaa tcttgtgctt gccagctcac
    22321 ttctggccgc cagcattccg gcatttgccg taaccggaga cactgatcag ccgatccaca
    22381 ttgaatcgga ccagcaatct cttgatatgc aaggcaacgt ggttaccttt accggtaatg
    22441 tcatcgtcac ccagggcacc atcaaaatta atgccgacaa agtggtcgtt acccgtccgg
    22501 gcggcgaaca aggtaaagaa gtgattgacg gatacggtaa accggcaacg ttctaccaga
    22561 tgcaggacaa cggtaaaccc gttgaaggtc acgcttccca gatgcactac gaactggcaa
    22621 aagattttgt cgttctgacg ggtaatgctt atctgcagca ggtcgatagc aacattaagg
    22681 gcgataagat cacttacctg gtgaaagagc agaaaatgca ggctttcagc gacaaaggca
    22741 agcgcgtaac caccgttctg gtgccgtcgc agctgcagga caaaaacaac aaaggccaga
    22801 ccccggcaca gaagaagggt aattaattcg ttatggcaac attaactgca aagaaccttg
    22861 caaaagccta taaaggccgt cgcgtggtag aagacgtcag cctgaccgtc aactccgggg
    22921 aaattgtcgg tctgctgggg ccaaacggtg ccggtaagac caccactttc tacatggttg
    22981 taggcattgt gccgcgcgat gcgggcaaca tcattattga tgatgacgat atcagtctgc
    23041 tgcctctgca tgcacgcgcg cgccgcggta tcggctatct gccacaggaa gcctccattt
    23101 tccgtcgcct cagcgtttac gataacctga tggcggtact gcaaattcgt gacgacttgt
    23161 ctgctgaaca acgtgaagac cgcgcgaacg agctgatgga agagtttcac attgagcacc
    23221 tgcgtgacag catggggcag tcactctccg ggggtgaacg tcgccgtgta gaaattgccc
    23281 gcgcactggc tgcgaatccg aaatttattc tgctcgacga accgtttgcc ggggttgacc
    23341 cgatctcggt tatcgacatt aaacgcatca ttgaacacct gcgcgacagc ggcctgggcg
    23401 tgctgatcac tgaccacaac gtgcgtgaaa cactggcggt ttgtgaacgc gcttatatcg
    23461 tcagtcaggg gcatttgatc gcccacggca cgcctacaga aatcttacaa gacgaacacg
    23521 ttaagcgtgt ataccttggg gaagacttca gactctgata gggtagaagt ttgcgacgtt
    23581 ttagcaggag agtacgattc tgaacatgaa gcaaggtttg caactcaggc ttagccaaca
    23641 actggcgatg acgccacagc tccaacaggc aattcgtctg ttgcagttgt cgacgctgga
    23701 acttcagcag gagctacagc aggcgctgga gagtaatccg ctgcttgagc aaatcgacac
    23761 tcatgaagaa atcgacaccc gcgaaacgca agacagtgaa acgctggaca ccgccgacgc
    23821 gctcgaacaa aaagagatgc cggaagagct gccgctcgat gccagttggg acaccattta
    23881 caccgctggt acaccatccg gcaccagcgg tgactacatt gacgacgagc tgccggtcta
    23941 ccagggcgaa acgacgcaga ccttgcagga ttacctgatg tggcaggtcg agctgacacc
    24001 gttttccgac actgaccgcg ctattgctac ctctatcgtc gatgccgttg atgaaaccgg
    24061 ttatctgact gtcccgctgg aagatattct cgaaagtatg ggcgatgaag agatcgacat
    24121 cgacgaggtt gaagccgtcc ttaagcggat ccaacggttt gatccggtcg gtgtagcggc
    24181 aaaagatctg cgtgactgcc tgctaatcca actctcccaa ttcgataaga ccacgccgtg
    24241 gctggaagag gccagattga tcattagcga tcatctcgat ctgttagcca atcacgactt
    24301 ccgcacttta atgcgcgtca cgcgtctgaa agaagatgtg ctgaaagaag ccgtcaatct
    24361 gatccagtcg ctcgatccgc gccccgggca gtcgatccag actggcgaac ctgagtatgt
    24421 cattccagat gtgctggtgc gtaagcataa cggtcactgg acggtagaac tcaacagtga
    24481 cagcattccg cgtctgcaaa tcaaccagca ctacgcctcg atgtgcaata acgcgcgcaa
    24541 cgatggtgac agccagttta tccgcagcaa tctgcaggat gccaaatggt tgatcaagag
    24601 tctggaaagc cgtaacgata cgctactgcg cgtgagtcgc tgtatcgttg aacagcagca
    24661 agccttcttt gagcaaggtg aagaatatat gaaaccgatg gtactggccg atatcgccca
    24721 ggctgtcgaa atgcatgaat cgacgatatc tcgcgtgacc acgcaaaaat acctgcatag
    24781 tccacgaggc atttttgaac tgaagtattt cttttccagt cacgtcaata ccgagggcgg
    24841 cggcgaagct tcctccacgg cgattcgtgc actggtgaag aaattaatcg cggcggaaaa
    24901 cccagcgaaa ccgttgagcg acagcaagtt aacctctttg ctgtcggaac aaggtatcat
    24961 ggtggcacgc cgcactgttg cgaagtaccg agagtcttta tccattccgc cgtcaaacca
    25021 gcgtaaacag ctcgtttgac ccaaccgata aggaagacac tatgcagctc aacattaccg
    25081 gaaataacgt cgagatcacc gaggcactgc gcgaatttgt tacagctaaa tttgccaaac
    25141 ttgagcaata ttttgaccga atcaaccaag tctatgttgt tctgaaagtg gagaaagtca
    25201 cccacacctc agatgcaaca ctgcatgtaa acggtggtga aattcatgcc agcgcggaag
    25261 gtcaggacat gtacgctgcc attgatggct taattgataa gctggcacgc cagttgacca
    25321 aacataaaga taaactgaaa caacactaat tgtccgggca attagcatgt gcatggcggc
    25381 ctgttgtgcg gcacaacggg ccatttgtac ggttaatgct ccgagcctgt tccactgttt
    25441 gagtgggcag gttcttaggt gaaattatga caaataatga tacaactcta cagcttagca
    25501 gtgttcttaa cagggaatgt acgcgaagcc gcgtccactg tcagagcaaa aaacgcgccc
    25561 tggaaatcat cagcgagctg gcggcgaaac aacttagcct gccgcctcag gtggtttttg
    25621 aagctatcct gacgcgggaa aaaatgggca gtaccggtat cggcaatggt attgccattc
    25681 cgcatggcaa acttgaagaa gatactctgc gtgccgttgg cgttttcgtt cagctcgaaa
    25741 cgcctatagc tttcgatgcc atcgacaacc aaccggtgga cttacttttt gccctgctgg
    25801 tgccggcaga ccaaactaaa acgcacctgc atactctgtc gctggtggcg aaacgtctgg
    25861 cggacaaaac catctgccgc cgtttacgcg cagcccagag cgatgaagag ctgtatcaaa
    25921 tcattacgga taccgaaggt actccggatg aagcgtagtt attcggtaat gtctctttta
    25981 gacgttgtga ggagaaacag tacatggtac tgatgatcgt cagcggacgt tcaggttcag
    26041 gtaaatctgt cgccctgcgt gcgctggaag atatgggttt ttactgcgtg gataaccttc
    26101 ccgtagtgtt gttacccgat ctggctcgaa ctctggccga tcgcgagatt tctgccgccg
    26161 tcagcattga tgttcgtaat atgccggagt caccagaaat attcgaacag gcgatgagta
    26221 acctgcctga cgctttctca ccgcaactac tgttcctgga tgccgaccgt aataccttaa
    26281 ttcgtcgtta cagtgacacg cgccgactgc atccgctttc cagcaaaaac ctgtcgctgg
    26341 aaagtgctat cgacaaagaa agcgatttgc tggagcctct gcgttcgcga gcggatctga
    26401 ttgtcgatac ctcagaaatg tccgttcacg agctggctga aatgctgcgt acccgtctgc
    26461 tgggtaaacg tgaacgcgaa ctgaccatgg tctttgagtc tttcggcttc aaacacggta
    26521 tccctatcga tgcagattac gtctttgacg tgcgcttctt gccgaacccg cactgggatc
    26581 cgaaactgcg tccaatgaca ggtcttgata aacctgtcgc cgcgttcctc gaccgccaca
    26641 cagaagtaca caattttatc taccagacgc gaagctatct tgagctatgg ttacctatgc
    26701 tggaaaccaa caaccgtagc tacctgacgg tcgccattgg ttgtaccggc gggaagcacc
    26761 gttcggtgta tattgcagag caactggcag actacttccg ctcgcgcggt aaaaacgtcc
    26821 agtcacgcca tcgtacgctg gaaaaacgta aaccatgacc gtcaagcaaa ctgttgaaat
    26881 cacaaacaag ctgggcatgc atgcccggcc tgcaatgaag ctgtttgaat taatgcaggg
    26941 ttttgacgct gaagtgctgt tacgtaatga cgaaggcacc gaggctgaag ccaacagcgt
    27001 tattgcgctg ctgatgttgg attctgccaa aggacggcag attgaagttg aagcgaccgg
    27061 tccacaggaa gaggaagcac tggccgccgt tatcgccctc tttaattctg gttttgatga
    27121 agattaatct tcatcacttt gacatacaac tcccttcaag ctcccccgac aataagaaaa
    27181 tcacgtactg aaatcgttct caatcaacgt catttgtaca ttttgtgcgc ttttcacttt
    27241 cagaagaacc ttaagaaaac cttaagaggt attgtttagg ttttgtttaa gttaatcgat
    27301 catactggtg atcgtcagaa aatatttcca ggagatggca tgattcgctt atctgaacaa
    27361 agtcccctgg gcaccggaag acatcggaaa tgttatgcgc atccggaaga tgcccaacgc
    27421 tgtatcaaga ttgtctacca tcgtggcgat ggtggcgata aagagatccg ccgtgagtta
    27481 aagtactacg cgcatcttgg tcgccggtta aaagactgga gtggaatacc gcgctatcac
    27541 ggtaccgttg agacggattg cggaaccggt tatgtctacg atgtgattgc cgattttgac
    27601 ggcaagcctt ccattacgct gaccgaattt gctgaacaat gccgttacga agaagacatt
    27661 gcacaactgc gccagctact gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg
    27721 acgatgtcgc tgaagccgca gaatatcctc tgtcaccgca tcagtgagtc agaggttatc
    27781 ccggtcgtct gcgacaacat tggtgaaagc acattgattc cactggcgac ctggtcgaag
    27841 tggtgttgtc tgcgtaagca agagcggtta tggaaacgat ttatcgccca accggcgctg
    27901 gctattgccc tgcagaaaga tctgcaacct cgtgagagca aaacgctggc cctcacttcg
    27961 cgcgaagctt aatccagctg gtgttgttgc ataaacggct cactgcctaa ctgatacatc
    28021 tgccgtaaaa tccacgcctg acggctacgc acgtagcccg atggcgagga gactttgaaa
    28081 cgaagtggat taggtaatac agctgccagt aatgcagctt ccgaccgggt aagtttgctc
    28141 gcgggtttgt ggaaataacg ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat
    28201 tcggcgatat tcaggtaaac cgtcaggata cgctttttgc tccagactgt ttctatcccc
    28261 agcgttaatc cagcttccag cccttttcgt acccagctac gcccatccca taaaaagaga
    28321 tttttggccg tctgctgaga aatcgttgaa gcaccgcgaa tacggttttc attgcgctcg
    28381 ttgtgcgcca gggctttctc aatggaagcg acatcaaagc cccagtgctc aggaaatttc
    28441 tgatcttctg cggcaatcac cgccagtccc atccacggcg agatttgatc catactgacc
    28501 cagtcagaat gtgccacgta acgaaaattg ccatgcagcc aggcgctgac ctgtcgctcg
    28561 accattaccg ctgagaaggg aacaggcgca acgctaaaca acgcgatgcc cccgccccag
    28621 aaaacggcga ggacaaccat taaccgcaaa agaaaacggc gaacgaaact aaacaccgtt
    28681 aagcggcttt tactcattca gccagaacca gcacgcggga aaccagctta tcaatgccgc
    28741 tcgccgcttc tgcaatgttc tgcgccagca tatatgctgg ggtggtgaca atcttattgt
    28801 cttcatcaac cacgatatca tcgacaggac acggcacatg ctccgcgccc atctcttcca
    28861 gcacttctgc ggtatcgata tcagtaccga tggtcaaacg cagcgggaaa tcgaaaattt
    28921 tcggcagcat cgccggggca atacacataa aaccaagcgg ttttccggcc tgatgcatcg
    28981 cttgtgccag ctcctttaat tcacggtcaa cggtgcattc gctaccaaga ctggcaaaat
    29041 tgcttaaatt cttcgccgcg ccaaaccccc ccggcacaat caacgcatcc agttcagcgg
    29101 catcggcctg ggccagagga cggatttcac cgcgcgttat tcgtgccgct tcaatcagca
    29161 cattgcgcgt ttccgtcatc gcttcgccag ttaaatggtt gataacatca acctgctgct
    29221 tatccggtgc aaagcagacc gcctgcgcac cgctgcgtga aatagctaac aacgtcaaca
    29281 ccgcttcatg aatttcagaa ccgtcataga cgccgcatcc gctcagaatt acgccaattt
    29341 tcttcattgt gatcatcctt tcgcaattga ctgaaacaca ttaatttttt taataaaaat
    29401 ggtacgcatc acacatttaa ctgattcatg taacaaatca tttaagtttt gctatcttaa
    29461 ctgcgtgcgg cctgaaaaac agtgctgtgc ccttgtaact catcataata atttacggcg
    29521 cagccaagat ttccctggtg ttggcgcagt attcgcgcac cccggtctag ccggggtcat
    29581 tttttagtgg cttttgccac ccacgctttc agcacttcta cgtcgtgacg ccactcttct
    29641 ttcatctctt caatccattc accgacgtta tcttcccagg cgggaaggtc aggagactga
    29701 atttgctgac ccagctgttg cagatggcgt aaccccactg accccgccgc acctttaatt
    29761 ttatgtcctt cctcaacaat gcctttttta tcctgcgccg tcagattcga ctccagcacg
    29821 ctgacatagc ccggcatcat cttctcaaac accgctaacc cgtcggtgat cagcttcggt
    29881 cctacaagtt cgagatactg ttccagcatg ggaatatcga gcaatgcttc tgatttactg
    29941 ttctcttctg tcgtcaccgt actctcctca tcatcctggg tatcccagaa tttcttgatc
    30001 atcgcggtta gcgccggaac cgaaagcggc ttactcagca catcatccat tccagcattg
    30061 aggtactctt gtttgtcttt cagcacgtta gcggttaagg ccaccagcgg cggtaaatcc
    30121 tcgcgcggat aacgcttcgt cagttcacga gagatatcca gcccggtcat atctggcaac
    30181 tgaatatcca gcaacaccag gtcgtattcg cccggtttaa acatctccag cgccgccttg
    30241 ccggtcatgg cgacatcaac gctgttacct aatttttcca gcacagaacg cgcgacaatc
    30301 acgttcagtt caatgtcttc caccagcagc acattcagcg ccggtaaagg catatcgtct
    30361 tcatcaaacg catcatcgac ctcttctgcc accgacggtg cgtggatcgt caacgtaaag
    30421 gttgaacctt tgccctgttc gctggtaacc gtaatatcgc cacccatatt tttcgccaga
    30481 cgacgagaaa cggccagacc aataccggtg ccggtggcag gtttaccgcc atgactgtct
    30541 ttcacctggt aatacatggc gaagatttta tccagctcat cctgcggaat gccgatacca
    30601 gaatcttcca cttcaaaatg cagcatatcg ccttcatcgt agcgcacgcg cacggtaacc
    30661 tggccttgct gggtgaattt gacggcgtta ctgatgaggt tccacaggat ctgccgtaaa
    30721 cgcgtcccgt cggtaatgac ctgatgcggt aatggcagcg taggctccag gttaaagcgc
    30781 aatccttttt gttgcgcctg caatgcggag agattttcca gatcggcaag gaagctggtg
    30841 aaatcaaccg gctgattatc aagctggacc ttgcgccgtt ccatcttatc catgtcgata
    30901 atatcgttaa agatattccc cagcgtgacg gccgaaacat ggatagtttt gagatatttt
    30961 tcctgctcgg cggtgagttc ggtatccagc agaatgcggc tcaggccgac gataccgttc
    31021 agcggtgtac gcaattcgtg actgatggtg gaaataaacg tcgttttgtc acggctggcg
    31081 cgttcaagcg catcctgata ccgcttacgc tcggtaatgt cgcgaccaaa gcccatcaaa
    31141 ccgtgacgtt tacccacgcg gtcgtagtac ggcactttac ggatttcaaa gcaggctttg
    31201 cgcccgtccg ggtaatccag ccactgttca taggtcagtg acacattatg acggaacact
    31261 ttttcatcgg tttcaatgac ttttgcggcg gcttccggtg agtaaacatc agcaggtttc
    31321 aggtgaacca gttgtttttc gctttttccg gtcagcagct ccatcgcgcg gttacagccg
    31381 gaaaactctt tatcttcgtt acgataaaaa accaggtcgg gtgaagcatc aaggaaggaa
    31441 cgtaagaatg aggattgctg ctcgagctga atttgtgtct cttcgcgctc tttgatttca
    31501 attttcagtt ggccgaaggt ttcctgcagt tctgcttccg ctttttcacg aacggcaatt
    31561 tcctgattta gctgggcaat attatttttt aactgaacgt tgaggctcaa atcgcgctcg
    31621 cgcatctcct ccagtttttg caccagccgt gacagacgtt gtcgtgactc ctccagttgc
    31681 tcgacgacca ccgatagaaa gtagaccgcc cacggcgtaa tcagcaaacc aaagaagata
    31741 gaacgaataa catcaatgct ttcgacctga ccatgcagca ccatggttac cgccatttgt
    31801 accacaatgg caagaacgac gagggccagc gccagcaaca ttgagaagcg caccagacct
    31861 aacttcatca tcaggtcaac ataatactgc gccagcagac gaatttgctt cattagggaa
    31921 ttccttcacg acaacctgcc ccaataatac ccaattctga cagttacgtt ataaattgtg
    31981 tgagaaatgc ggaattcccc tcaacgacct actccgttgg aggtagccac ggacgtccca
    32041 gcgccgatcc ctgtacgcca tgttcattca gatagcgatc cagctcgacc attcccgtcc
    32101 atcgattttc gcaccacagc ggagcaagca gcgtcgggcg acgggcgctg gcggaaatgc
    32161 ggtggtagat cacttccggt ggcgtatggc gaatcatttc tccggcagtg agcgtataat
    32221 cctccagttc aataccgttc aaacgccccg cttcccaggc tttcgccata atgctgcctt
    32281 tcacaatatg cagcggatgc agctttatgc cgtccacgcc ggtttcaacc actcgctcca
    32341 gcgtttgcaa acattcagcc tgcccttcgc caggcaggcc gacaattaag tgggaacaca
    32401 ccttcagccc gcgctgacgt gccagctggg ttgtacgctg ataacaggca aaatcatggc
    32461 cgcggttgat gcgatgcagt gttttgtcgt gggcggtttg tagccccagc tccagccaca
    32521 cttcgtaacc ctggtcctta tattcgcaaa gcagatccag caccgcatcc ggcacgcagt
    32581 ccgggcggat accaacacac aaaccgacaa tattggcctg gctcaccgcc tgctgataca
    32641 tagaacgcag cacctgaact tccgcaaagg tgctggtata cgcctgaaag taggccagat
    32701 agcgtttagc gcggttaact aaattcgcct ggtgcgccag ttgctcggca atggaacgat
    32761 gctgctgcgc ttcatcggca aacgaggcaa cattacagaa tgtgcagcca ccacgcccga
    32821 tggtaccgtc acggttaggg cagctaaaac cgccgtgcag cgtcagctta tgcacctttt
    32881 gcccataacg acgggtgaga tcaccaccaa acatattgac taatttctgt aactgcataa
    32941 tctgatagac cgcgccttga aaagaggcca aagcctgcca tttttagctc aattcggcga
    33001 tgacctggat caatcgtccc ggcctgcttt tatcaactgc ataatcaatc aaaattaccg
    33061 aaatttcatg cataatcaca taaatcactt ttgcttatct tgtgtcagat ttttttatct
    33121 ccttatggat tttaggcaaa aacagtagca tgaaacgtca ttaccaatta aggcagtata
    33181 aaatgctggt tttctcgtca gttcaaggca ggataagggt taacacgcct ttatgacagt
    33241 caggaattga ctgtttctct aacgactctc cttttagcct taaagataaa atctatttta
    33301 atttcagcta tttaataaag aattttgcgc taaagcacat ttctgtacca ataagcttgc
    33361 catttgacct gtatcagctt tcccgataag ttggaaatcc gctggaagct ttctggatga
    33421 gcagcctgct catcatattt atgcagtaat tgagatcccc tcttcaccgt attaaccgat
    33481 gcgaaaagga caacaagggg gcgaatgcga ggcgcgcgta tgacacgcaa accccgtcgc
    33541 cacgctcttt ctgtgcccgt gcgcagcggt tcggaagtgg ggttcccgca gagcctaggg
    33601 gaggttcacg atatgttgta cgataaatcc cttgagaggg ataactgtgg tttcggcctg
    33661 atcgcccaca tagaaggcga acctagccac aaggtagtgc gtactgcaat acacgcactg
    33721 gcccgcatgc agcaccgtgg cgcgattctc gccgatggta aaaccggcga cggttgcggc
    33781 ttgctgttac aaaaaccgga tcgctttttt cgcatcgttg cgcaggagcg cggctggcgt
    33841 ttagcaaaaa actacgctgt cgggatgctc ttcctgaata aagatcctga actcgccgct
    33901 gccgcacgcc gcatcgttga agaagaactg caacgcgaaa ccttgtcgat tgtgggctgg
    33961 cgtgatgtcc ccactaacga aggcgtgctg ggtgaaatcg ccctctcctc tctgccacgc
    34021 attgagcaaa tttttgtgaa cgccccggca ggctggcgtc cacgcgatat ggagcgccgt
    34081 ctgtttatcg cccgccgccg cattgaaaag cgtctcgaag ccgacaaaga cttctacgtc
    34141 tgtagcctgt cgaatctggt gaacatctat aaaggtctgt gtatgccggc ggatctgccg
    34201 cgcttttatc tggatcttgc ggacctgcgt ctggaatcgg ccatttgcct gttccaccag
    34261 cgcttctcca ctaacaccgt accgcgctgg ccgctggcgc aaccgttccg ctatctggcg
    34321 cataacggtg aaatcaacac catcaccggt aaccgccaat gggcgcgtgc gcgtacctat
    34381 aaattccaga cgccgctgat ccctgacctg cacgacgccg cgccattcgt caacgaaacc
    34441 ggttctgact ccagttccat ggataacatg ctggaactgc tgctggcagg cgggatggat
    34501 atcatccgcg ccatgcgcct gttagtgcca cccgcctggc agaacaaccc ggatatggac
    34561 ccggaactgc gtgccttctt tgactttaac tccatgcata tggagccgtg ggatggtccg
    34621 gcgggcatcg tgatgtccga tggtcgcttc gctgcctgta acctcgaccg taacggtctg
    34681 cgtccggcac gctacgtcat taccaaagac aagctcatca cctgcgcctc tgaagtcggt
    34741 atctgggatt accagcctga cgaagtggtc gaaaaaggcc gcgtcggacc aggcgaactg
    34801 atggtgatcg acactcgcag tgggcgtatt ctgcactcgg cagaaaccga tgacgatctg
    34861 aaaagccgcc acccttataa agagtggatg gagaaaaacg tccgccgact ggtaccgttt
    34921 gaagatctgc ccgatgaaga agtgggtagc cgcgaactgg acgacgacac gcttgccagc
    34981 taccagaaac agtttaacta cagcgcggaa gagctggact ccgtaattcg cgtactgggc
    35041 gaaaacggtc aggaagcggt cggttcgatg ggcgatgata ccccattcgc cgtgctctcc
    35101 agtcagccgc gcattattta cgactacttc cgccagcagt ttgcccaggt gactaacccg
    35161 ccaatcgacc cgctgcgtga agcgcatgtt atgtcgctcg ccaccagtat cggtcgtgaa
    35221 atgaacgtct tttgcgaagc agagggccag gcgcaccgtt taagctttaa atcgccgatt
    35281 ctgctctact ccgatttcaa acagctcacg acgatgaaag aggagcacta ccgcgcagat
    35341 acgctggata tgacctttga cgtcactaaa accacgctcg aagcgacagt caaagagctg
    35401 tgcgacaaag ccgaaaaaat ggtacgtagc ggcaccgtgt tgctggtgct ctccgaccgg
    35461 aatatcgcta aagatcgcct gccggttccg gctccgatgg cggttggcgc gatccagacc
    35521 cgtctggtcg atcaaagcct gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc
    35581 cgcgatccgc accacttcgc cgtgttgctg ggtttcggcg cgacggctat ttatccgtac
    35641 cttgcctatg aaacgctggg ccgcctagta gacacccacg cgattgccaa agattatcgt
    35701 accgtgatgc tcaactaccg taacggcatc aacaaaggct tgtacaaaat catgtccaaa
    35761 atgggcatct ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtcta
    35821 cacgatgatg tagtgggcct gtgcttccag ggcgcggtca gccgcattgg tggggcaagc
    35881 tttgaagact tccagcagga tctgctgaat ctgtcgaaac gtgcctggct ggcgcgcaag
    35941 ccgattagcc agggcggcct gctgaaatac gtccacggcg gcgaatacca cgcctacaac
    36001 ccggacgtgg tgcgcacgct gcaacaggcg gtacaaagcg gcgagtacag cgactatcag
    36061 gaatacgcga agctggttaa tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt
    36121 acgccgggtg aaaacgcggt caacattgct gatgttgaac cggcaagcga actgtttaaa
    36181 cgctttgata ccgccgcgat gtctatcggc gcgttaagcc cggaagccca cgaggcgctg
    36241 gcggaagcga tgaatagcat cggcggtaac tcgaactccg gtgaaggcgg cgaagacccg
    36301 gcgcgctatg gcactaacaa agtgtcgcgc atcaagcagg tggcttctgg tcgcttcggc
    36361 gttactccgg cgtatctggt caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg
    36421 aagccgggcg aaggcggtca gttgccgggc gacaaagtca ctccttacat cgccaaactg
    36481 cgctattcgg tgcccggagt gacgctgatc tccccgccgc cgcaccacga tatctactct
    36541 atcgaagact tagcgcagct tattttcgac ctcaagcagg ttaacccgaa agcgatgatc
    36601 tccgttaagc tggtgtccga accaggcgtt ggcaccatcg cgactggcgt ggcgaaagct
    36661 tatgcggact tgatcaccat cgcgggctat gacggcggca ccggcgcaag cccgctgtcg
    36721 tcagtgaaat atgcaggctg cccgtgggaa ctcggtctgg tggaaaccca acaggcgctg
    36781 gttgccaacg gcttgcgtca taaaattcgt ttgcaggttg atggcggcct gaaaacgggt
    36841 gtcgatatca tcaaagcggc gattctcggc gcagaaagct tcggcttcgg cactggccca
    36901 atggtggcgc tcggctgtaa atatctgcgt atttgccacc tgaacaactg cgccacgggt
    36961 gtagcaactc aggatgacaa actgcgtaag aatcactatc acggcctgcc gttcaaggtg
    37021 actaattact ttgagtttat cgcccgtgaa acccgcgagc tgatggcaca gctgggagta
    37081 acgcgtctgg tggatctgat tggtcgcacc gacctgctga aagagctgga cggtttcacc
    37141 gccaaacagc agaaactggc gctgtcgaag ctgctggaga ctgccgagcc acatgcaggt
    37201 aagtcactct actgcactga aaacaacccg ccgtttgata acggcctact gaacgcgcag
    37261 ttgctgcaac aggcgaaacc gtttgtcgat gaacgccaga gcaaaacctt ctggttcgat
    37321 atccgcaaca ccgaccgttc tgtcggcgcg tcgctttcag gctatatcgc ccagacgcac
    37381 ggcgatcagg ggctggcagc cgatcctatc aaagcgtact tcaacggcac cgcaggccag
    37441 agtttcggtg tgtggaacgc gggcggcgtg gaactgtacc tgaccgggga tgccaacgac
    37501 tatgtcggta aaggcatggc gggcggctta atcgccattc gtcctccggt tggttccgcc
    37561 ttccgcagcc atgaagcaag cattatcggc aacacctgcc tgtatggcgc gaccggtggt
    37621 cgtctgtatg ccgcaggccg cgcgggtgaa cgtttcggcg tgcggaactc cggtgctatc
    37681 accgtggtag aaggcattgg cgacaacggc tgtgaatata tgacgggcgg tattgtctgt
    37741 attctgggta aaaccggcgt taacttcggc gcgggcatga ccggcggctt cgcttacgtt
    37801 ctcgatgaaa gcggtgattt ccgcaaacgc gttaacccgg aactagtcga agtcttaagc
    37861 gtcgacgatc tggcgatcca tgaagagcat ctgcgcggtc ttatcaccga gcatgttcag
    37921 cataccggtt ctcagcgcgg tgaagagatt ctggcgaact ggtcaacctt cgccacgaaa
    37981 ttcgcgctgg ttaaaccgaa gtccagtgat gtaaaagcac tgctgggtca ccgtagtcgt
    38041 agcgcagcag agttgcgcgt gcaggcgcag taaggggtag caacaatgag tcagaatgtt
    38101 tatcaattta tcgacctgca gcgcgttgat ccgccaaaga aaccgctgaa gatccgtaaa
    38161 attgagtttg ttgaaattta cgagccgttt tccgaaggcc aggccaaagc gcaggctgac
    38221 cgctgtctgt cgtgcggcaa cccatactgc gagtggaaat gcccggtaca caactacatc
    38281 ccaaactggc tgaagctcgc caacgagggg cgtatttttg aagcggcaga actgtcgcat
    38341 cagaccaaca cactgccgga agtatgcgga cgtgtctgcc cgcaagaccg tctgtgcgaa
    38401 ggttcctgca ccctgaacga tgagttcggt gcggtgacca tcggcaacat tgagcgttat
    38461 atcaatgata aagcgttcga aatgggctgg cgtccggata tgtccggcgt gaagcaaacc
    38521 ggtaaaaaag tggcgattat cggtgcaggc ccggcaggtc tggcgtgtgc ggatgtcctg
    38581 acgcgtaacg gcgtaaaagc cgttgtcttc gaccgtcatc cagaaattgg cgggctgctg
    38641 accttcggta ttccggcctt caagctggaa aaagaggtaa tgacgcgtcg ccgtgaaatc
    38701 ttcaccggca tgggtattga attcaaactc aataccgaag tgggccgcga cgtacagctg
    38761 gacgatctgc tgagtgatta cgatgccgtg ttccttggcg ttgggactta tcagtcaatg
    38821 cgcggcgggc tggaaaacga agacgccgat ggcgtgtacg cagcgctgcc gttccttatc
    38881 gccaacacca aacagttaat gggctttggt gaaacccgcg acgaaccgtt cgtcagcatg
    38941 gaaggcaaac gcgtggtagt acttggcggt ggcgacactg cgatggactg cgtgcgcacg
    39001 tctgtacgcc agggggcaaa gcacgttacc tgtgcctatc gtcgtgatga agagaacatg
    39061 ccgggttccc gccgcgaagt gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac
    39121 gtccagccac tggggattga agtgaacggt aacggcaaag tcagcggcgt aaaaatggta
    39181 cgtaccgaaa tgggcgaacc ggatgccaaa ggccgtcgcc gcgcggagat cgttgcaggt
    39241 tccgaacata tcgttccggc agatgcggtg atcatggcgt ttggtttccg tccacacaac
    39301 atggaatggc tggcaaaaca cagcgtcgag ctggattcac aaggccgcat catcgctccg
    39361 gaaggcaacg acaacgcttt ccagaccagc aacccgaaaa tctttgctgg cggcgatatc
    39421 gtccgtggtt ccgatctggt cgttaccgcc atcgccgaag gtcgcaaagc ggcagacggc
    39481 attatgaact ggctggaagt ttaagctctg tcccagcgcc gcctcctcag aaaagagtcg
    39541 gcgctgaaat tctcctttct ttctggaaat cgcctcgcat aatctgaaga aaaccaatgt
    39601 aatacgctca tctggattgt taccggtaga tgcccggcgt atagctgatt ccattattca
    39661 acgcagcgcc atataaaggg acagataatg gaatcgctct cagagagaac ctcaacaggc
    39721 taccagcaaa tccacgacgg cattattcat ctggtcgata gcgcccgaac ggaaacggta
    39781 cgtagcgtta acgcgctgat gaccgcgacg tactgggaaa ttggccgacg gattgtcgaa
    39841 tttgaacaag gtggtgaggc cagagcagct tatggtgcac agttgattga tcggctatcg
    39901 aaggatttaa gtcgacggta taaacgtggg gtctctgcaa aaaacttacg ccaaatgagg
    39961 cttttttacc tcttttttca acatgttgaa attcgccaga cagtgtctgg cgaattaaca
    40021 ccattgggaa ttccgcagac accatctgca gaattcccat cggcaaagat ttggcagacg
    40081 ctgtctgcca aattgagagg ctgtttgata tttccacagc ttttgtattg catctcctat
    40141 tttcggtttg tatattcatg caaataaacg aaggaa
//
LOCUS       DBKEKI010000024        39732 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.024, whole genome shotgun sequence.
ACCESSION   DBKEKI010000024 DBKEKI010000000
VERSION     DBKEKI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.024"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..963
                     /locus_tag="ACUQPX_001206"
     CDS             <1..963
                     /locus_tag="ACUQPX_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HHV9055970.1"
                     /translation="FAVSNGRIEATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMD
                     TRVLQEQRLEAIAQIKEAQSAVAAAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHT
                     RSRSLAQRGAISAQQLDDDRAAAESARAALESAKAQVSASKAAIEAARTNIIQAQTRV
                     EAAQATERRIAADIDDSELKAPRDGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFF
                     LPTEQAGTLKLGGEARLILDAAPDLRIPATISFVASVAQFTPKTVETSDERLKLMFRV
                     KARIPPELLQQHLEYVKTGLPGVAWVRVNEELPWPDDLVVRLPQ"
     gene            960..3695
                     /gene="rbbA"
                     /locus_tag="ACUQPX_001207"
     CDS             960..3695
                     /gene="rbbA"
                     /locus_tag="ACUQPX_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HHV9055971.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            3695..4819
                     /locus_tag="ACUQPX_001208"
     CDS             3695..4819
                     /locus_tag="ACUQPX_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9055972.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            4878..5153
                     /locus_tag="ACUQPX_001209"
     CDS             4878..5153
                     /locus_tag="ACUQPX_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HHV9055973.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            5150..5509
                     /locus_tag="ACUQPX_001210"
     CDS             5150..5509
                     /locus_tag="ACUQPX_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HHV9055974.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(5629..6030)
                     /gene="nikR"
                     /locus_tag="ACUQPX_001211"
     CDS             complement(5629..6030)
                     /gene="nikR"
                     /locus_tag="ACUQPX_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HHV9055975.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(6036..6842)
                     /gene="nikE"
                     /locus_tag="ACUQPX_001212"
     CDS             complement(6036..6842)
                     /gene="nikE"
                     /locus_tag="ACUQPX_001212"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HHV9055976.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(6839..7603)
                     /gene="nikD"
                     /locus_tag="ACUQPX_001213"
     CDS             complement(6839..7603)
                     /gene="nikD"
                     /locus_tag="ACUQPX_001213"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HHV9055977.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(7603..8436)
                     /gene="nikC"
                     /locus_tag="ACUQPX_001214"
     CDS             complement(7603..8436)
                     /gene="nikC"
                     /locus_tag="ACUQPX_001214"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HHV9055978.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRMSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYSRMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(8433..9377)
                     /gene="nikB"
                     /locus_tag="ACUQPX_001215"
     CDS             complement(8433..9377)
                     /gene="nikB"
                     /locus_tag="ACUQPX_001215"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HHV9055979.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPVLDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(9377..10951)
                     /gene="nikA"
                     /locus_tag="ACUQPX_001216"
     CDS             complement(9377..10951)
                     /gene="nikA"
                     /locus_tag="ACUQPX_001216"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HHV9055980.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(11062..11649)
                     /gene="acpT"
                     /locus_tag="ACUQPX_001217"
     CDS             complement(11062..11649)
                     /gene="acpT"
                     /locus_tag="ACUQPX_001217"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HHV9055981.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(11704..12753)
                     /locus_tag="ACUQPX_001218"
     CDS             complement(11704..12753)
                     /locus_tag="ACUQPX_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HHV9055982.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RNLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            12852..14102
                     /locus_tag="ACUQPX_001219"
     CDS             12852..14102
                     /locus_tag="ACUQPX_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9055983.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPEHVPGAASSS"
     gene            complement(14106..14663)
                     /gene="dcrB"
                     /locus_tag="ACUQPX_001220"
     CDS             complement(14106..14663)
                     /gene="dcrB"
                     /locus_tag="ACUQPX_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HHV9055984.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(14736..15401)
                     /locus_tag="ACUQPX_001221"
     CDS             complement(14736..15401)
                     /locus_tag="ACUQPX_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HHV9055985.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            15622..15867
                     /gene="tusA"
                     /locus_tag="ACUQPX_001222"
     CDS             15622..15867
                     /gene="tusA"
                     /locus_tag="ACUQPX_001222"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HHV9055986.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(15969..18167)
                     /gene="zntA"
                     /locus_tag="ACUQPX_001223"
     CDS             complement(15969..18167)
                     /gene="zntA"
                     /locus_tag="ACUQPX_001223"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HHV9055987.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(18241..18867)
                     /locus_tag="ACUQPX_001224"
     CDS             complement(18241..18867)
                     /locus_tag="ACUQPX_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HHV9055988.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRVDNAIAAACYFAGHFLIVRSLYL"
     gene            19008..19367
                     /locus_tag="ACUQPX_001225"
     CDS             19008..19367
                     /locus_tag="ACUQPX_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HHV9055989.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(19370..19639)
                     /locus_tag="ACUQPX_001226"
     CDS             complement(19370..19639)
                     /locus_tag="ACUQPX_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HHV9055990.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(19629..20225)
                     /gene="rsmD"
                     /locus_tag="ACUQPX_001227"
     CDS             complement(19629..20225)
                     /gene="rsmD"
                     /locus_tag="ACUQPX_001227"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HHV9055991.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            20375..21871
                     /gene="ftsY"
                     /locus_tag="ACUQPX_001228"
     CDS             20375..21871
                     /gene="ftsY"
                     /locus_tag="ACUQPX_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HHV9055992.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            21874..22542
                     /gene="ftsE"
                     /locus_tag="ACUQPX_001229"
     CDS             21874..22542
                     /gene="ftsE"
                     /locus_tag="ACUQPX_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HHV9055993.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            22535..23593
                     /gene="ftsX"
                     /locus_tag="ACUQPX_001230"
     CDS             22535..23593
                     /gene="ftsX"
                     /locus_tag="ACUQPX_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HHV9055994.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            23838..24692
                     /gene="rpoH"
                     /locus_tag="ACUQPX_001231"
     CDS             23838..24692
                     /gene="rpoH"
                     /locus_tag="ACUQPX_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HHV9055995.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            24963..26052
                     /gene="livJ"
                     /locus_tag="ACUQPX_001232"
                     /pseudo
     CDS             24963..26052
                     /gene="livJ"
                     /locus_tag="ACUQPX_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
     gene            complement(26251..26634)
                     /gene="panM"
                     /locus_tag="ACUQPX_001233"
     CDS             complement(26251..26634)
                     /gene="panM"
                     /locus_tag="ACUQPX_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HHV9055996.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTALQGGWEKR"
     gene            27058..28167
                     /gene="livK"
                     /locus_tag="ACUQPX_001234"
     CDS             27058..28167
                     /gene="livK"
                     /locus_tag="ACUQPX_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HHV9055997.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLLNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            28215..29141
                     /gene="livH"
                     /locus_tag="ACUQPX_001235"
     CDS             28215..29141
                     /gene="livH"
                     /locus_tag="ACUQPX_001235"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HHV9055998.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            29138..30415
                     /gene="livM"
                     /locus_tag="ACUQPX_001236"
     CDS             29138..30415
                     /gene="livM"
                     /locus_tag="ACUQPX_001236"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HHV9055999.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            30412..31179
                     /gene="livG"
                     /locus_tag="ACUQPX_001237"
     CDS             30412..31179
                     /gene="livG"
                     /locus_tag="ACUQPX_001237"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HHV9056000.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            31181..31894
                     /gene="livF"
                     /locus_tag="ACUQPX_001238"
     CDS             31181..31894
                     /gene="livF"
                     /locus_tag="ACUQPX_001238"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HHV9056001.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            32291..33607
                     /gene="ugpB"
                     /locus_tag="ACUQPX_001239"
     CDS             32291..33607
                     /gene="ugpB"
                     /locus_tag="ACUQPX_001239"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HHV9056002.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            33705..34592
                     /gene="ugpA"
                     /locus_tag="ACUQPX_001240"
     CDS             33705..34592
                     /gene="ugpA"
                     /locus_tag="ACUQPX_001240"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HHV9056003.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNQVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            34589..35434
                     /gene="ugpE"
                     /locus_tag="ACUQPX_001241"
     CDS             34589..35434
                     /gene="ugpE"
                     /locus_tag="ACUQPX_001241"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HHV9056004.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            35493..36426
                     /locus_tag="ACUQPX_001242"
                     /pseudo
     CDS             35493..36426
                     /locus_tag="ACUQPX_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            36423..37166
                     /gene="ugpQ"
                     /locus_tag="ACUQPX_001243"
     CDS             36423..37166
                     /gene="ugpQ"
                     /locus_tag="ACUQPX_001243"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HHV9056005.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(37153..37593)
                     /locus_tag="ACUQPX_001244"
     CDS             complement(37153..37593)
                     /locus_tag="ACUQPX_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HHV9056006.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            37713..39455
                     /gene="ggt"
                     /locus_tag="ACUQPX_001245"
     CDS             37713..39455
                     /gene="ggt"
                     /locus_tag="ACUQPX_001245"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HHV9056007.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(39514..>39732)
                     /locus_tag="ACUQPX_001246"
     CDS             complement(39514..>39732)
                     /locus_tag="ACUQPX_001246"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHV9056008.1"
                     /translation="KNRGVASALMREMIEMCDNWLRVDRIELTVFVDNAPAIKVYKKF
                     GFEIEGTGKKYALRNGEYVDAYYMARVK"
BASE COUNT         9178 a        10347 c        11146 g         9061 t
ORIGIN      
        1 tttgctgtca gcaatgggcg cattgaagcg acggaagtgg atatcgccag caaaattgcc
       61 gggcgtatcg acaccattct ggtgaaagaa ggccagtttg ttcgcgaagg cgaagtgctg
      121 gcgaagatgg atactcgcgt gttgcaggaa cagcgactgg aagccatcgc gcaaatcaaa
      181 gaggcacaaa gcgccgttgc cgccgcgcag gctttgctgg agcaacgaca aagtgaaact
      241 cgtgccgcac agtcgctggt caatcaacgc caggccgaac tggactccgt tgccaaacgt
      301 catacgcgtt cccgctcgct cgcccaacga ggggctattt ctgcgcaaca gctggatgac
      361 gaccgcgccg ccgctgagag cgcccgagct gcgctggaat cagcgaaagc ccaggtttcg
      421 gcttctaaag cggctataga agcagcacgc accaatatca ttcaggcgca aactcgggtc
      481 gaagcagcgc aagccactga acggcgcatt gccgcagata tcgatgacag tgaactgaaa
      541 gccccgcgtg acggacgcgt gcagtatcgg gttgccgagc caggggaagt gctggcggca
      601 ggcggtcggg tgctgaatat ggtcgatctc agcgacgtct atatgacctt cttcctgcca
      661 accgaacagg cgggcacgct gaaactgggc ggtgaagccc ggctgatcct cgatgccgcg
      721 ccagatctgc gtattcctgc gaccatcagt tttgtcgcca gtgtcgccca gttcacgcca
      781 aaaaccgtcg aaaccagcga tgaacggctg aaactgatgt tccgcgtcaa agcgcgtatc
      841 ccaccggaat tactccagca gcatctggaa tatgtcaaaa ccggtttacc gggcgtagcg
      901 tgggtgcggg tgaatgaaga acttccgtgg cctgacgacc tcgtggtgag gttgccgcaa
      961 tgacgcatct ggaactggtt cccgtcccgc ctgtcgcgca actggcgggc gtgagccagc
     1021 attatggaaa aaccgttgcg ctgaacaata tcactctcga tattccggcc cgttgcatgg
     1081 tcgggctgat tggtccggat ggcgtcggga agtcgagttt attgtcactg atttccggtg
     1141 cccgcgtcat tgagcagggc aatgtggtgg tgctgggtgg cgatatgcac gacccgaagc
     1201 atcgccgaga cgtctgcccg cgcatcgcct ggatgccgca ggggctgggc aaaaacctct
     1261 accacacctt gtcggtgtat gaaaatgtcg attttttcgc tcgcctgttc ggtcacgata
     1321 aagcggagcg ggaagtgcga attaatgagc tgctgaccag caccgggtta gcaccgtttc
     1381 gcgatcgtcc ggcaggtaaa ctctccggcg ggatgaagca aaaacttggg ctgtgctgcg
     1441 cgttaatcca cgacccggaa ctgttgatcc ttgatgagcc aacaacgggg gttgacccgc
     1501 tctcccgcgc ccagttctgg gatctgatcg acagtattcg ccagcggcag agcaatatga
     1561 gcgtgctggt cgccaccgcc tatatggaag aggccgaacg cttcgactgg ctggtagcga
     1621 tgaatgccgg agaagtgctg gcaaccggca gcgccgaaga gctgcggcag caaacgcaaa
     1681 gcgccacgct ggaagaagca tttattaatc tgttaccgca agcgcaacgc caggcgcatc
     1741 aggcggtagt gatcccaccg tatcaacctg aaaacgcaga gattgccatc gaagcgcgcg
     1801 atctgaccat gcgttttggt tccttcgttg ccgttgatca cgtcaatttc cgcattccac
     1861 gcggggagat ttttggattc cttggttcga acggctgcgg taaatccacc accatgaaaa
     1921 tgcttaccgg actgctgccc gccagcgaag gtgaggcgtg gctatttggg caaccggttg
     1981 atccaaaaga tatcgatacc cgccgtcgtg tgggctatat gtcgcaggcg ttttcgctct
     2041 ataacgaact caccgtgcgg caaaacctgg agttacatgc ccgtttgttt cacatcccgg
     2101 aagcggaaat tcccgcgcga gtggctgaaa tgagcgagcg ttttaagctc aacgacgttg
     2161 aagatgttct gccggagtca ttgccgctcg gcattcgcca gcggctttcg ctggcggtgg
     2221 cggtgattca tcgcccggag atgttaatcc tcgatgagcc tacttctggt gtcgatccgg
     2281 tggcgaggga tatgttctgg cagttgatgg tcgatctctc gcgccaggac aaagtgacta
     2341 tcttcatctc cacccacttt atgaacgaag cggaacgttg cgaccgcatc tcactgatgc
     2401 acgccggaaa agtgcttgcc agcggtacac cgcaggaact ggttgagaaa cgcggagccg
     2461 ccagtctgga agaggcattt atcgcctatt tgcaggaagc ggcagggcag agcaacgaag
     2521 ccgaagcgcc gcccgtggta cacgacacca cccacgcgcc gcgtcaggga tttagcctgc
     2581 gccgtctgtt tagctacagc cgccgcgaag cgctggaact gcgacgcgat ccagtacgtt
     2641 cgacgctggc gctgatggga acggtgatcc tgatgctgat aatgggttac ggcatcagta
     2701 tggatgtgga aaacctgcgc tttgcggtgc tcgaccgcga ccagaccgtc agtagccagg
     2761 cgtggacact caacctctcc ggttcccgtt actttatcga acagccgccg ctcaccagtt
     2821 atgacgagct tgatcgtcgg atgcgtgcgg gcgatatcac ggtggccatt gagatcccgc
     2881 ccaatttcgg gcgcgatatc gcgcgtggta cgcctgtgga actcggcgtc tggatcgacg
     2941 gagcgatgcc cagtcgtgcc gaaacggtaa aaggttacgt gcaggcaatg caccagagtt
     3001 ggcttcagga tgtggcgagc cgacaatcga cacccgccag ccaaagcggg ctgatgaaca
     3061 ttgagacgcg ttatcgctat aatccggacg taaaaagcct gccagcgatt gttccggcgg
     3121 tgatcccgct tctgctgatg atgatcccgt caatgctaag cgcccttagc gtggtgcggg
     3181 aaaaagagct agggtcgatt atcaaccttt acgtgacccc caccacccgt agcgaatttt
     3241 tgcttggcaa acagctgccg tacatcgcgc tggggatgct gaactttttc ctgctctgcg
     3301 gcctgtcggt gtttgtgttt ggcgtaccgc ataaaggcag tttcctgacg ctcaccctgg
     3361 cggcgctgct gtatatcatc attgccactg gaatggggct gctgatctcc acctttatga
     3421 aaagccagat tgccgccatt ttcggaacgg cgattatcac attgatcccg gcgacacagt
     3481 tttccgggat gatcgatccg gtagcttcgc tggaagggcc tggacgttgg atcggcgagg
     3541 tttacccgac cagtcatttt ctgactatcg cccgcggaac gttctcgaaa gcgctggatc
     3601 tgactgattt gtggcaactt tttatcccgt tgctgatagc catcccgctg gtgatgggct
     3661 taagcatcct gctgctgaaa aaacaggagg gatgatgcgc catttacgca atatttttaa
     3721 tctgggtatc aaagagttgc gcagtctgct cggtgataaa gcgatgctga cgctgattgt
     3781 cttctcgttt acggtgtcgg tgtattcgtc agcgaccgtt acgccaggat cgttgaacct
     3841 cgcgccgatc gccattgccg atatggatca atcgcagtta tcgaaccgga tcgttaacag
     3901 cttctatcgt ccgtggtttt tgccaccgga gatgatcacc gccgatgaga tggatgccgg
     3961 actggacgcc ggacgctata ccttcgcgat aaatattccg cctaattttc agcgtgatgt
     4021 cctcgccgga cgccagccgg atattcaggt gaacgtcgat gccacgcgca tgagccaggc
     4081 atttaccggc aatgggtata tccagaatat tatcaacggt gaagtgaaca gctttgtcgc
     4141 gcgctaccgt gataacagcg aaccgttggt atcgctggaa acccggatgc gctttaaccc
     4201 gaaccttgat cccgcgtggt ttggcggggt gatggcgatc atcaacaaca ttaccatgct
     4261 ggcgattgta ttgaccggat cggcgctgat ccgcgagcgt gaacacggca cggtggaaca
     4321 tttactggtg atgccgataa cgccgtttga gatcatgatg gcgaagatct ggtcgatggg
     4381 gctggtggtg ctggtggtat cgggattatc gctggtgctg atggtgaaag gtgtactggg
     4441 cgtaccgatt gaaggctcga tcccgctgtt tatgctgggc gtggcgctca gtctgtttgc
     4501 caccacgtca atcggcattt ttatggggac gatagcgcgt tcaatgccgc aactggggct
     4561 gctggtgatt ctggtgctgc tgccgctgca aatgctttcc ggtggatcca cgccgcgcga
     4621 aagtatgccg cagatggtgc aggacattat gctgaccatg ccgacgacac actttgttag
     4681 cctcgcgcag gccatcctct accggggtgc cggattcgaa atcgtctggc cgcagttcct
     4741 gacgctgatg gcaattggcg gcgcgttttt caccatcgca ctgctgcgat tcaggaagac
     4801 gattgggaca atggcgtaag gaagggaaac ttgacttcat actgtgatgt cataaagtga
     4861 agtcataagg acatgccatg agaaccaagg tacaggcttt acggaagaaa caaaaaaata
     4921 cattggatca aatatttaaa actcctgttc ctcaagggat caagtggtct gatatagagt
     4981 cactggttaa agcattaggc ggagaaatta aggaaggaag aggttcgcgt tgcaagttca
     5041 tactaaatat gagcgttgcg tgtttccatc ggcctcatcc gtcgccagat accgataaag
     5101 gcgctgtaga aagcgtgcgt gactggttac taagtatagg ggtaaaacca tgatcaaact
     5161 gaagacgcca aactctatgg agattgccgg gcaaccagct gttattacct acgtgccaga
     5221 actgaatgca tttcgtggca aattcctggg cttgtcaggt tattgtgatt ttgtctctga
     5281 cagtattcag gggctgcaaa aagaaggaga gctttccctg cgagagtatc ttgaggattg
     5341 taaagctgcg ggtattgagc cgtatgcgcg tacagaaaag attaagacct ttacgttacg
     5401 ctatccagaa tcgctgagtg aacgccttaa taatgcggct gcgcagcaac aagtctctgt
     5461 taatacctat attattgaaa cactgaatga gcgtttgaat catttataaa ttttagcgac
     5521 gtacattacg cagaatatct gcctttacag gatctatcta tgcaactgaa cttaatttta
     5581 taaattaagt tcagttaata tcttttcttt ccttgtttta gaaataactc aatcttcctt
     5641 cggcaggcac tgcaaatgcc cgtgccgcac gccgcgctgg gcgataacgt catcggcaaa
     5701 atgctgcacg tcacccatgt cacctttcaa cacggcgatt tccagacagt cgtcgtggtt
     5761 gatgtgcaca tgtagcgtgg cgacggagag gtcgtggtga tgatgctggg tggaaacaat
     5821 acggctggct aagtcgcgtt tttcgtgttc atacacatac gacagcaccg cgaaaccttg
     5881 tgtgccgtgc tgctgggtag cttcctgcgc cagggcacta cgcagaatat cgcggatagc
     5941 ttcggaacgg ttgttataac cacgacgctg gctcaggctg tccagcgtct ccagtaaatc
     6001 gtcatcaagc gtgatggtga ctcgttgcat tggcgttaaa ccttttctga ggtgcgacgg
     6061 cgcacgggga atgcgggtaa taccgcgttt tgtagcacac gtccggcgtc agaggaaaag
     6121 gttaatttat ctcccaccgc ctgggtttcg acgatttgtc cgttgtccat caccatcact
     6181 cgctggcaaa agcgttccac caggcgtaag tcatgagtga tgaacaggca ggcagtgcca
     6241 aactgttgtt gtagcttttt cagcaggcga atgacgcccg cctgtaacac gagatcaagg
     6301 ttagaaacgg cttcatccag aatcagcaat ttcggttcga ccgccagcgc gcgtgccagg
     6361 cagacgagct ggagctggcc gccgcttaac tgcggtgggc gtttgtcgag aacgctgtca
     6421 tcgagatcga ccgccttcag catttcgctg gcgcgcgcca gttgttcgga ttttttcagt
     6481 gatagcaggt ggcgcatcgg ttcacgcagg atctcgcgca cggttttgcg cggattcacg
     6541 gcactgatgg agtcctgaaa caccatctga atatcacggc ggaaggcttt acgttgggcg
     6601 cgattaagtt tcgccagcga ttcgccgcgc cagctaatat tcccttgtga gggagactct
     6661 aagcctacca gcagccgcgc gagggtgctt tttccgcagc cgctgcgtcc cagcagggcg
     6721 acggtttcgc cgcttttcag ggtcagggaa acgttattca gcaccgcctg atgttgatgt
     6781 tttccgctaa atccaccgtg ggcatagtga tgggaaaggc cgcagacgtt aagtaaggtc
     6841 atgatgccag ctccataccg tagagggcga gatgagcgga aaccaggctg cgcgtcaccg
     6901 tatgtttggg ggcgttaaac agcgtttcta catcgccctg ttcgacaatt ttaccttgtg
     6961 acatcaccgc cacgtcatcc gccagacgcg ccacaacgcc catatcatgg gtgaccagca
     7021 gcattcccgg cgcttgtttt tgcataatgc tttccagcag atcgaggatg cgcgcctgtg
     7081 ctaccacgtc gaggtcggtg gtcggttcat cggcgatgat aaacggtgat tcacacagca
     7141 ccgccatcgc aatcatcatg cgctgcaaca tgccgccgct catctcgaac gggtacagct
     7201 tcagcacgcg cgcggcgttt tccagcccca ccgcttctat ggcagcggta agcgtagcgt
     7261 catcggcggg tttccctaac gccaggcagg tttcccgcgc gtgggtgtgc atggtgtgca
     7321 gtggattaaa ggcgctgcgc gggttctgca tgatggtggc aattttgatg ccgcgcaggg
     7381 cgcaaggcga aaccggtttg ccatcggcta aaatttcccc cgccgtctgg cgaacgccag
     7441 cgggcaaaat gcccagcgtc gcggcgcagg ttaatgattt cccgctgccg ctaccgccga
     7501 ctaacgccag cacgcgcccg cgttgcaggg ttaacgatac accgtgtacc agcggctgcg
     7561 cggcctgtag cgcgatatta cgtagttcaa tctgttgcgg cattagtgtg cgtgctccgt
     7621 caccagatga ggatccagat gatcgcgcag tgcgtcaccc accaggttaa aggccatcac
     7681 gctgataaac agcgccagcc ccggccagaa catttgcagc ggctgggtcc agatatactg
     7741 gcgcgcgtcg ttaatcatca cgccccattc ggcggtcggc gcggtcacac cgaggccaag
     7801 gaaagacatc cccgcgacgt gcagcatcat atggccgata tccagcgttg ccagcaccag
     7861 cagcgaaggg atcaccgcgc ctgccagatg atcgacaaac acccgcacat ggcccgcacc
     7921 ggaaagccgt gacgccagca caaactcgcg ttggcgtagt gaaatcacca agctgcgcac
     7981 catgcgtgaa taccacgccc agtgcgacag ggcgatggcg ataattacgt tggtcagccc
     8041 ggtgccgagc acgccaacca taaagaacga cagaatcgag gtcgggaagg tcataaacat
     8101 atcggcgacg cgcatggtgg cctgatcaac gcgcccgcca atcaacccgg cgctgccgcc
     8161 aataaccagc cctaatgtca gcaccagcag caggcaggcc attaccgaac cgagcgacat
     8221 gcgggtcgct gccatcagcc gcgagaaaat atcgcgacct aagtgatcgg tgcccagcca
     8281 gtgctgcgca tccggcgaaa gcaggcacga cggcaaatca attgcctgtg gatcgtacgg
     8341 cagccaccac tggctggtga gcgcaatcag cgccagcagg gcgataatga tcagcgccag
     8401 gcgtaccgac cagcgggaag agaggaaaaa gttcacgcgt gcgctccttc atgacgacga
     8461 atgcgcgggt ccagcgcggc gttgagcaaa tcgacaatca aattacagac cacaaaaacc
     8521 accaccatca tcagcgtaaa gcactgaatc accggatagt cacggttaaa aatcgccgac
     8581 accgcatagc gcccgacgcc aggccaggca aagatgtttt cgataatcat cgtcccgcca
     8641 atcaattcgc caatgtgcat ccccacggcg gtgatcatcg gcagcgaggc attgcgcaga
     8701 atatggcggc gttcggtctg tttgtcattc aggccgcgta gccgcgccca ggtgacgtga
     8761 cgctgaccgg cgacgtccag catactggcg cgcagcagtc gcgcgttaat cgccagcgac
     8821 ataaaggcga tggaaaccgc aggtaaaatg atgtgctgcc agccgccgta gcccatcgcg
     8881 ggtagccatt gcagatacac cgaaaacgcc atcactagca aaaatgccag ccagaagttg
     8941 ggcatcgaca cgccaagaaa cgcgataaag cgcacggcga aatccggcaa acgatcgcgg
     9001 tggcgcgccg cccagatacc gagcggtacg gaagtgagca gaattaatac cagcgccgca
     9061 cctgcaagtt ccagcgtggc gggcaggaag ttcagcatat cgtccagtac cgggcgttgg
     9121 ctggcgaatg agataccaaa gtcaagatgc agcgccttcc acaaccaggt accgtactgg
     9181 acgtacagcg gctgatccag cccgagcatg gtgcgggtag aggccagcat ctccggcgtc
     9241 ggcggcaggt tagataaacg cagataatcg agcaccgggt cgccggtacc gaggcgcagc
     9301 atcagaaaaa tgatcaccga ggcggcgagc accatcggga tcagcagcag aaagcggcgt
     9361 aatacgtaac gcaacattaa ggtttcaccg gtttaatctg ttcgaacgga atttcggtgg
     9421 cgatcggcgc gtaggggata ttacccagct ccggttttga taccaccatc atcgagatgt
     9481 agctgatagg cagataaacc gcctcgtcat gcagacgggt cagaatgtcg cgatacagcg
     9541 cctgacgttg cgtttcgtca tgggtcgcca gcacttcgcc gatctcttta tcaatcagcg
     9601 gcttgtcggc taatccttgc tgtgcctgga agtcagcgtg cgacggtacg cgcattgaac
     9661 tcaggaaggc gtgtggatcg tatggcgcgc cccaggtgcg gtggaaaatc atgccaaaac
     9721 gaccgtcgcg ctgacgagca tagatactgc tctcttcttc gccaatcagc gagacatctg
     9781 cgccaatctg gcgcatatca gcctgaatga tttccgccat cgatttgctt aacgcatcgg
     9841 tgccgatgaa cgacagttca atgcgcagcg gctgaccatt tttctcgcgg atgtctttgc
     9901 ccgcaggcag cgtccaaccg gctttttcca gcaactcttt cgctttctgc ggatcgtact
     9961 ggcgcggttt caggccgaga ttagcgtaag gcacagaagg ggcaaacagg gtgtcggcga
    10021 cctgctgggt gccatataac gcgttatcaa tcagcgattt tttgtttacc gcgtaattaa
    10081 gagcttcacg tactgccagc tcgttggtgg gggctttggc ggtattgagc gccagcatca
    10141 cggtttcgat cggctgtgac agttgggtgt ggtaagccgg attctggcta aagcgggcga
    10201 aggtatcgag cggtaataac ccttcgtttc cgtacagcag gtcgatatcg ccagtttcaa
    10261 acgccaccgc gcgggtagtc gggtccggga tgacgttaaa ggtgatcttt ttaatcgctg
    10321 gcttttcgcc ccagtagttt tcgttacgga cgaagacatc gtactgattc agtttcgatt
    10381 cctgcaaaat ccacggtccg gtgccaatcg gcgctttaat tccgttcatg gtttcatggt
    10441 ttttaaactg cgagggagcg ataaaacgga aaggacgggg cagggccagt tcttgcagga
    10501 aaggatagta ggcgcttttc agggtaattt gcagctctgt tttactgagt gctttaacat
    10561 caacaatctg gtttgccagc tccagccagg cgtgacgttg acggttatcg agcactgcgc
    10621 ggaagttttc tgccgccgcc tcggcatcga acggttcacc gttggagaat ttcacgtcat
    10681 cacgcagggt gaaggtccag gttttaccat cttctgaatg agtccagctt tttgccagcc
    10741 acgggatcac cgaaccgtct gcctgatatt tcaccaatgg ttcataaacc atgctctggg
    10801 cgaacatctg gttaggcgtg taaaggtgcg ggtttagtgg cccgacattc accggccagg
    10861 cggtggtgat ttcatctggt gcagcggcat ggacgataaa agacgcacaa gccagcagcg
    10921 caaatagagt gcggcggagt gtggagagca tggtaacccc aatggattaa aatagatggc
    10981 ggaaataagt atgacgattt taagtattcg tcatactgat tacctgttct ggatcaatag
    11041 atgggcagtc gggtgggcgg atcagttaac tgaatcgatc cattgcacac tgtcggcggt
    11101 gagcgtaaag ggggtggggg tgcagatcgc aaggcttaaa ttttctagct gacaatggct
    11161 gactgacagc gaggagtgat aggtgctatc tacgctgacg atttgccagg cgctgccgcc
    11221 acgctgttta acaatggctt ctttgcgcgt ccagatgcgc cagaacattt ccagttgctg
    11281 atcaggatgc acggcgtcca tttcagcgtg ttccccgagg ctgaacacgg cgttcgccag
    11341 ccagcgccag ttggcgcgcg ggcgaatcac ttcgatatcg cagccgactt cgccttcatc
    11401 gctcaacagc agagcgatat cgtcaccgct atggcttaag ttgaaccata gcggcgtttc
    11461 cggcgcgaat gcaggtttgc cttgttcgcc atagatgatc tccggtagcg gggaaagcgt
    11521 gtgcgaaagc aatgcacgcc ccgccagcca gcgttcgcgt cgtggacctt gcggtgcttg
    11581 ctcgcgtaaa cccggtggca gtggagctgc gcttaaggtc gaaactttcc ccagaactat
    11641 ccgatacata tcagggccaa cgtttaatgg aaaatgaaag tgcgtatcgt atcacttgtc
    11701 gcctcatccc ggtaaccgac ttttcggtct gcccggcccc agtaaaatcg ccagtttgct
    11761 gccacctttc gtcgtttcca tccagatttt acacacgctg gttaatggca ccgaaagtag
    11821 catccctacc gggccgagca gccatcccca gattaacaat gaaagaaata ccaccatggt
    11881 ggacatcccc aggcgatggc ccatcatccg tggttctaaa atattgccga tgaccatatg
    11941 gaccactaaa aacaatgcgc cgaccagaat acattcgtaa acaccattaa acagcagcac
    12001 ctgaatcatt ggcggtacgg cggaaattac cgcgccgata ttgggcacgt agttgagcaa
    12061 aaacgccagt actgcccaca tcagcgcaaa ctgtacgccc attagcgcca gccccagcca
    12121 gacgatgaca cctgtccata aactgagtag cgtctttaac gccagataat gcgaaacgcc
    12181 tttaagtgcg cggtgtaatc ctgcgatgtg aatctgtggg ttattcagcg caaaacgcat
    12241 tttgtaaggg acgtggcgca cttcaaacag cataaaaact acggtcatca ccagcaaaag
    12301 cacgctcgcc attgccccgg aaagcccggt cattagcgct gtggtgaagg taaccacttt
    12361 ttccgagtcc atccgctgca gcattcgctc cggcgacata tgcaaattaa gaaaaggcaa
    12421 catctcctgc aatttaaaaa ggttgcgcgt cagctcctta ttaaacttcg gcagcataga
    12481 gataaattcg ttaaacgatg ccgccagtac gccgaccagc gcggttagtg cgatcagcat
    12541 caccaccact acaatcgtaa tggcaacggg gcgttgtact ccccgacgaa taaaccaggt
    12601 aacgagcggg ttgaggacga tggcaaaaaa cagcgccagt aacagctgca caatgatatc
    12661 tgccgctgcg tgaatgcccg cgaggatcac taccagcgag gccaacttga gcagaatgtg
    12721 catgcccgtt ttatcgggtt gaggggtttc cattggggct tccttgtgac tttttgtgtt
    12781 aagtgtagcg ggagtcacgc cagcaatatt ctgattctta ctgctgattt tcacgtcagc
    12841 acatggtaaa aatgaaacac tgttgtaaaa atgtggtgat cctcatgccc gaacccgtag
    12901 ccgaacccgc gctaaacgga ttgcgcctga atttgcgcat tgtctccatt gtcatgttta
    12961 acttcgccag ctacctcact atcgggttgc cgctcgctgt attaccgggc tatgtccatg
    13021 atgtgatggg ctttagcgcc ttctgggcag gattggttat cagcctgcaa tatttcgcca
    13081 ccttgctgag ccgccctcat gccggacgtt acgccgattt gctgggaccc aaaaagattg
    13141 tcgtcttcgg tttatgcggc tgctttttga gcggtctggg gtatctgacg gcaggattaa
    13201 ccgccagtct gcctgtcatc agcctgttat tactttgcct ggggcgcgtc atccttggga
    13261 ttgggcaaag ttttgccgga acgggatcga ccctatgggg cgttggagtg gttggctcgc
    13321 tgcatatcgg gcgggtgatt tcgtggaacg gcattgtcac ttacggggcg atggcgatgg
    13381 gtgcgccgtt aggcgtcgtg ttttatcact ggggcggctt gcaggcgtta gcgttaatca
    13441 ttatgggcgt ggcgctggtg gccattttgt tggcgatccc gcgtccgacg gtaaaagcca
    13501 gtaaaggcaa accgctgccg tttcgcgcgg tgcttgggcg cgtctggctg tacggtatgg
    13561 cgctggcact ggcttccgcc ggatttggcg tcatcgccac ctttatcacg ctgttttatg
    13621 acgctaaagg ttgggacggt gcggctttcg cgctgacgct gtttagctgt gcgtttgtcg
    13681 gtacgcgttt gttattccct aacggcatta accgtatcgg tggcttaaac gtagcgatga
    13741 tttgctttag cgttgagata atcggcctgc tactggttgg cgtggcgact atgccgtgga
    13801 tggcgaaaat cggcgtctta ctggcggggg ccgggttttc gctggtgttc ccggcattgg
    13861 gtgtagtggc ggtaaaagcg gttccgcagc aaaatcaggg ggcggcgctg gcaacttaca
    13921 ccgtatttat ggatttatcg cttggcgtga ctggaccact ggctgggctg gtgatgagct
    13981 gggcgggcgt accggtgatt tatctggcgg cggcgggact ggtcgcaatc gcgttattac
    14041 tgacgtggcg attaaaaaaa cggcctccgg aacacgtccc tggggccgcc tcatcatctt
    14101 aaaacttact gaataaccag cgtattaatg atgttttctg cggtggtttg cgctttttgc
    14161 tgatcgtcag ctggcagcgt aatttgcatg gtcagcagtt gattacccac gttacccaga
    14221 ataacggaag agtacgccgt ctggcctttc gcggagataa tactgtctaa ctgctgcatt
    14281 ttgtgacctt tcagctcaat ggctttattg gttaccactt gcagctgcgg atcgcggcta
    14341 cgttgctgat cttccagacg cttcgccaac accgccagat cttctttcgg atcatcgccc
    14401 atgatgacga tgactgcttt ctgcccggtg gcgtcggacc agacatgcat gttattggcc
    14461 tgcgttccca gcttaccgct ctggtcggtc atatccgctg gcagcgagaa acttaacttg
    14521 ccatcaagca ggttgacggg attcccggta gcgttacttt ccgcgaccga accctgcgcc
    14581 gtagcgttag tgtctttatc atcacaggcc gcaagcccca taaccagcag gccaattccg
    14641 acatatttaa ccagattgcg cattgacttc ttcctttcga taaacggcca taacggctca
    14701 ttcatccatc ttatcacaac tctgataacg aacctttaac tcgcctgcaa agcgttgatt
    14761 tcggatttat ctgccagtct tttcaacagc atattgagta atacgccata cattggcagg
    14821 aagaaaacga tactgattaa cactttgaaa cagtaatcga ccagcgcgat ttccatccag
    14881 tgttcagcca taaaggcatc cgggctacgc cagaaggcaa tgaagaaaaa ggccagcgtg
    14941 tcgctgacgt taccgaacag tgtggacgct gtcggtgcca gccaccagcg gcgactctga
    15001 cgcaggcggt taaaaacgtg cacgtcgagg atttgcccca gcgcgtaggc catgaaactg
    15061 gcggtggcga tacgggcgac aaacaggttg aagtgggcga gtgcgccgaa tccctgccag
    15121 gaacccatat agaatagcga cgagatgacg taggagatta ataacgcagg gatcattacc
    15181 gcgaagataa tgcgtcgggc cagcggtgcg ccaaaaatac gcacggtcag gtcggtagca
    15241 agaaaaataa acggaaagct aaacgcgccc caggtggtat ggaaaccaaa aatggagacg
    15301 ggaagctgaa ccagatagtt actggaggtg atcaccagaa gatgaaataa cgataaccag
    15361 aacaacgcct tatagcgttg agtttgcgag aaaacgttca tattgtacct ttttgattaa
    15421 ccattggggt gagggaaccc aatacgtacg acacgtctgt taccttaaca taataacgtg
    15481 cttacccttt tttcgagccg ccgcatgata ctgctttacg ttgacaatgc aatggttaat
    15541 tttcacgcaa tcgttaacct agtttgctta cggactcaca gggcgtaaac tagcgccgtt
    15601 tttttaagtg atgagaagaa aatgaccgat ctcttttcca gccctgacca cacactcgac
    15661 gcgcttggcc tgcgctgccc ggaaccggtg atgatggtgc gcaaaaccgt gcgcaatatg
    15721 cagcctggcg aaacgttgct gattatcgcc gacgatccgg ccactacccg cgatattcct
    15781 gggttttgta cctttatgga acacgaactg gttgcgaaag agacggatgg actgccttat
    15841 cgttatttga ttcgtaaagg cggttgatag gggctgattg gcttcgatgc cgccttttcc
    15901 tcacccccta accctctccc cagaggggcg aggggaccga tcgcgctcaa tgttgcgatc
    15961 ggtttgcctt atctcctgcg caacaacctt aacgcattcg ccgtcaccag caccgtcgca
    16021 cccgtatctg ccagcactgc cagccacaac ccggtcatcc ctaaaagcgt ggtgacgagg
    16081 aagatccctt tcagtcccag cgcaatggtg atgttctggc ggatattggc gtgagtggcg
    16141 cgtgccagtt caatcatttg caccaggccg cgcaggtggt tatgggttaa tgctgcgtcg
    16201 gcggtttcca gcgccacgtc tgtgccgcta cccatagcaa tcccgatggc ggcagctttc
    16261 atcgctggcg cgtcgttaat accatcaccg accatcgcca ggggcgcatg ttgattcagc
    16321 ttggtcaccg ctttgacttt atcttccggc aataggcccg ctttaaactc cagccccagc
    16381 tccccggcaa ttgccgccgc tgcgcgtgga ttatcgccgg tgaggatcac tcctttaacc
    16441 cccagcgcgt tcagttcact gatggcagtt gcagcgtcgg cgcgcagggt atcctgcaat
    16501 gcaatgatac ccagcacgtc atcattacgt actaccagca caaccgtttg cccggcgctt
    16561 tccagttcat taatcaaacc agcaaatgca tcagcgggat gtttcccggc agcgcatatc
    16621 aacacgcgct caccattaac ctgcgcttca atgccagacc cgaccagtgc ccgctgtgat
    16681 tcggcggtgg gaatggcgag ttcagcaacc tgtgcttcgc gtacgatggc ttgcgccagt
    16741 ggatgcgtcg cgccttgctc gaccgccgcc gccagtgtca gcagttcaga ttcactaata
    16801 cccgttgccg gatgaatcgc ggtaacgcgc ggtttaccga cggtcagcgt accggtttta
    16861 tcaaacgcca cttgggtaac acgacctagt tgttccagcg ccgcaccgcc tttaattaac
    16921 gccccacgac gcgctgctgc tgccagcccg gaggttatcg ccgcaggcgt tgagataact
    16981 aacgcacacg ggcaaccaat cagcagcagc gtcagacctt tataaatcca ctcctgccag
    17041 ctggcggcaa acagcagcgg cggcactagc gttaccagca gggcgacggc cataatcgct
    17101 ggcgtataaa tacggctgaa acggtcgata aaccgctcaa tgggggcgcg gcgctcttcg
    17161 gcttcttcga tcagtttcag aatccggtca atggcgctgg cacccggttc tgacagcact
    17221 tccagcgtta ccagacggtc tacgctggtg gctcctgcag gaactttatc gcccgtcgcg
    17281 cgctccaccg gaatggattc tccggtcagg gcgctttcat caaaactggc aaacggtgag
    17341 agcagtttac cgtcggcagg caaacgccca cctgcggcga cttcaatcac atcgccaggc
    17401 cgcaggctgt taatcgccac ctcttcccgc tcaccgttac gcaggcgcgt ggcggtttct
    17461 ggtttcagcg ccattaacgc gctaacgccc tgacgcgcgc ggctggcggc ccagccttcc
    17521 agtcgttcac caatcaaaaa cagcagcaac accatcgcag cttcagccgt tgcgccaata
    17581 aacagtgcac caatagcggc tacgctcatt aaggtttcaa tggcgaagta gctgccggat
    17641 ttgatcagcc gtaatgcctg acgagcaatc gggtacagcc caactagcgt ggtcgcgata
    17701 aacgccagtt gcccgaacgg atgattgaac tgctccagac cccagctgat tgccatcatc
    17761 acgataagcg taatcagcgg cagattctct ttcaggcgcg atgcttgtgg ctcgtcggcg
    17821 gcctgctcat cgcgcaggga atagcccgct ttttgcaccg cagattcaac ctgggcacgg
    17881 atgtcattgt cggcatcaac caccagtttt tcggtggcga acaacacctg cacctgattc
    17941 acgcctgcaa gctggcgcac ggcattttct accttgcgcg cacaggcggc gcagtccatg
    18001 ccgctgactt tccagctata gcgggtgccg gagacgtttt cagagagcgt tggcgagctg
    18061 gagcatgcgc cgtcgcagca acagtcgttg gcgttctgta ccgtggttag cggtttgaac
    18121 gcagcaaatt gaggggcttt cttgccgtga ttgtcaggtg tcgacatggc atcctccggt
    18181 taagtttttt ctcattaacc gaaggataca ctctggagtc gactccagag tcaagtttta
    18241 tcagagatac agtgagcgga cgatgaggaa gtgaccggca aagtagcagg ccgcagcaat
    18301 cgcgttatcc acacggaagc gtcggcgata gtggctcccc agccagacaa agttactgat
    18361 aaacagcaac gaagcgccaa caaacgcaga gagcgccgga gcggtcggac ggaagaacca
    18421 cagttcacct gccagccaca ccatcaccag cgtcatgccg ataaacgtgc agataggcca
    18481 acggtactct tccagccgcg tccagataat cgccagtagc agcgcaccca gaaccagcaa
    18541 caccagtggc agaggccaga agaaagagag cgtcatctga ctggcgaaat agatggtgta
    18601 cagcaggtgc gagaggaaaa acgcgccgat ggcgtacatc agacgttgac gtggcaacag
    18661 ggttagcgca tcgcccagca gtgaggcgca cagtcccgcc agcaccagat agctaatggc
    18721 gtcgaacatc ggtgcttgcc aggccagtaa cagcaggaga agaagggtta acggtttaaa
    18781 cacccagcgt tgccaggttg gcccacgata cgatgcatcc acagatagcc atgcggaaag
    18841 acagacagcg ataaacgacc aaagcatctt agttccttga attcgttatt ttttcgtaag
    18901 cggaatgcct acggtcttgc ttccagttta gtggcgaggg tggacggatg acaacaccat
    18961 aactggcagg gtatgcttaa ttccgcattt tccgatgagg gataaagatg agtaaaccac
    19021 ctcttttctt tattgttatc attggcttaa ttgtcgtcgc cgcatcgttt cgttttatgc
    19081 aacagcgacg ggaaaaagct gataatgata tggctccgct ccagcaaaag ctggtggtgg
    19141 tgagcaacaa gcgggaaaaa ccgattaacg atcgccgttc gcgccagcag gaagtgactc
    19201 cggcaggcac cagtatgcgc tatgaggcaa gcttcaaacc gcaaagcgga ggaatggagc
    19261 agacgtttcg cctcgacgcc cagcagtacc acgccctgac agtgggcgat aaaggtacgc
    19321 tgagctataa aggaacgcgc tttgttagct ttgttggcga acaataaggt tattttttaa
    19381 ctttaaattt cttctgccag accagcagtt caaacacgcc gaaaaggaaa atccgcacct
    19441 tttcggcggt ggtcatctgc gggccatctt tcggtaaagt ggatttcaat agcgccagct
    19501 gcataccatg catcagcacg gtaaaaatca gcgccacgtt aacgaaaata tttagcgggc
    19561 gcgggaaggg atgcaccaga ttgaggatta aaaatcccca aacgcagagc attaacaaac
    19621 gaccaatatt aatcagcatc actttctcct tgtgcttcgt gttgatacag ccgataagcc
    19681 acctgacccg ccactttttc ccgatgtaat gaccagtttg ccggaacagt gggcagaccg
    19741 ttttcgactt cgctttcgac ataaatcagg gcttcgtcag ccagccagcc gttatcttcc
    19801 agtaaattta tcgtctcttc taacaagcca cggcggaacg gtggatcgac aaacacgata
    19861 ttatgcggtg tgcctttttg cgccaggaat gacattgcgt tgctgttcac cacgcgtgca
    19921 ttgcctgctt ttagtgtcgc cagattctta attaactgct gagaaaccac gcgatccatc
    19981 tcaatcaacg ttgcccccgc agcgtagcgc gataacgctt ccagccccag cgcgccgctc
    20041 ccggcgaagc aatccagaca ttgggcgtca acaatgaccg gagccagcca gttaaacaac
    20101 gtttcgcgta cgcggtcggt ggtggggcgc agacctgggc tatcaggaac cgggagttta
    20161 cggcctcgcc actgcccgcc aataatgcgg atttggccgc tgccggaatg attcggtttt
    20221 ttcatgataa attgctcaat ccgccagata acacatactt gcaggcggtg atgtgaatta
    20281 agttaagtga tagactattt catcattttt ttagctgcta tgtacatagc gttaacgctg
    20341 tgccatgaag caacagcgag gagtgtagtc gcaaatggcg aaagaaaaaa aacgtggctt
    20401 tttttcctgg ctgggctttg gtcaaaaaga gcagaccccg gaaaaagaga cagaagttca
    20461 gaatgaacaa ccggttgtag aagaaatcgt tcaggcgcaa gagcctgcga aggcttctga
    20521 acaagccgtt gaagagcagc cgcaggcgca tactgaagcc gaggcggaaa cttttgctgc
    20581 cgacgttgtg gaagtcactg aacaggttgt tgaaagtgaa aaagcgcagc ctgaagcgga
    20641 agttgttgca cagccggaac cggtcgtaga agaaacgccg gagccagtgg ctatcgaacg
    20701 tgaagagctg ccgttgccgg aagacgtcaa cgccgaagag gtttcgccag aagagtggca
    20761 ggctgaagcg gaaaccgtag agattgtcga agcggcagaa gaagaagcgg ctaaagaaga
    20821 aatcaccgac gaagagctgg aagcacaggc gctggctgcc gaagcggcag aagaggcggt
    20881 gatggtagtt cctccggcag aagaagagca gccggttgaa gaaattgccc aggagcaaga
    20941 aaaaccgacc aaagaaggct tcttcgcgcg cctgaaacgc agcctgttaa aaaccaaaga
    21001 aaatctcggt tccggattta tcagcctgtt ccgcggtaaa aaaatcgacg atgatctgtt
    21061 tgaagagctg gaagaacagc tgttgatcgc cgatgtgggc gtggaaacca cacgtaaaat
    21121 tatcaccaat ctgacggaag gcgcatcccg caagcagctt cgtgatgccg aggcgctcta
    21181 tggcctgctg aaagaagaga tgggcgagat tctggcgaaa gtcgatgagc cgctgaatgt
    21241 tgaagataaa acgccgttcg tgatcctgat ggtgggcgtc aacggtgtgg gtaaaaccac
    21301 gacgattggt aagctggcgc gtcagtttga gcagcagggt aaatcggtga tgctggcggc
    21361 gggcgatact ttccgtgcag ctgcggttga acagcttcag gtctggggtc agcgcaacaa
    21421 tattccggtg attgcccagc ataccggtgc ggattccgcc tctgttatct tcgacgccat
    21481 tcaggcagct aaagcgcgta atatcgacgt cctgattgcc gataccgccg gacgtctgca
    21541 gaacaaatcg catctgatgg aagagttgaa gaaaatcgtc cgcgtgatga agaaactcga
    21601 cgttgaagcg ccgcatgaag ttatgctgac tattgatgcc agcaccgggc agaacgcggt
    21661 aagccaggcc aaactgttcc acgaagctgt tggcttaacc ggcatcacgc taacgaaact
    21721 ggacggcacg gcgaaaggcg gggtaatttt ctcggtggct gaccagtttg gtatccctat
    21781 ccgctacatt ggtgtcggcg aacgtattga ggatttgcgt ccgtttaagg cggacgactt
    21841 tatagaggca ctttttgccc gagaggatta acaatgattc gctttgaaca tgtcagcaag
    21901 gcttatctcg gtgggagaca ggcgctgcag ggcgttacgt tccatatgca gccgggtgag
    21961 atggcgtttc tgaccggtca ttccggcgca gggaaaagta ccctcctgaa gctgatctgt
    22021 gggattgagc ggcccagcgc cgggaaaatc tggtttagcg gccatgacat cacgcgtctg
    22081 aaaaaccgtg aagttccgtt tctgcgtcgc cagattggca tgattttcca ggatcaccat
    22141 ctactgatgg accgtactgt ctacgataac gtggcgatcc cgctgattat cgctggtgcc
    22201 agcggtgacg atattcgtcg ccgggtgtcg gcggcactgg ataaagtcgg gctactggac
    22261 aaagcgaaga acttccctat tcagctttcc ggcggtgaac aacagcgtgt tggcattgcc
    22321 cgcgcggtgg tgaataagcc cgcggtactg ctggcggacg aaccgaccgg taacctggac
    22381 gacgcgttgt cggaagggat tctacgtctg tttgaagagt ttaaccgcgt tggggtaacc
    22441 gtattgatgg caacgcacga cattaacctg atttcacggc gttcctatcg catgctcacc
    22501 ctgagcgatg gtcacttgca tggaggcgtg ggccatgaat aagcgcgatg caatcaatca
    22561 tattcggcag tttggtgggc gtctggatcg cttccgtaaa tcggtcggcg gctcaggcga
    22621 cggcggtcgt aacgcaccga aacgcgcgaa atcctcgcca aaaccggtaa atcgcaaaac
    22681 caacgttttc aacgaacagg tgcgctatgc cttccacggc gcattgcagg atctgaaaag
    22741 caaaccgttc gccacgtttt taacggtgat ggttatcgcc atctccctga cgctgcccag
    22801 cgtctgttat atggtgtaca aaaacgttaa tcaggcggcg acgcagtatt atccgtcacc
    22861 gcaaatcact gtttacttgc aaaaaacgct ggacgatgac gccgctgcgg gcgtggtggc
    22921 acagttgcag gccgagcaag gcgtggagaa agtgaactat ctttctcgtg aagacgcact
    22981 gggcgagttc cgtaactggt ctggttttgg tggtgcgctg gatatgctgg aagaaaaccc
    23041 gcttccggca gtggcggtgg tgatcccgaa actcgatttt caggggacgg aatcactcaa
    23101 tacgctgcgt gatcgtatca cgcagattaa cggcattgac gaagtgcgga tggatgacag
    23161 ctggtttgcc cgtctggcgg cgttgaccgg gctggttggg cgcgtttcgg cgatgatcgg
    23221 cgtgttgatg gtggcggcag tgttcctcgt catcggcaac agcgtacgtc tgagcatctt
    23281 tgctcgccgt gactccatca acgtgcagaa actgattggt gcgacggatg gtttcatcct
    23341 tcgcccgttc ctgtacggcg gtgcgctgct gggattttct ggcgcattgt tgtcattaat
    23401 tttgtcagaa attctggtac tgcgtttgtc gtcagcggtg gcggaagtgg cacaggtttt
    23461 cggaacgaag tttgatatca atggtttatc gttcgatgaa tgcctgctat tgctgctggt
    23521 atgctcgatg attggctggg tggcagcgtg gcttgccacg gtacaacatt tacgccactt
    23581 tacgcctgaa taataaaagc gtgttatact ctttccctgc aatgggttcc gtagcaggga
    23641 aagagacccc gttgtctctt cccagtattt catctctatg tcacattttg tgcgtaattt
    23701 attcacaagc ttgcattgaa cttgtggata aaatcacggt ctgataaaac agtgaatgat
    23761 aacctcgttg ctcttaagct ctggcacagt tgttgctacc actgaagcgc cagaagatat
    23821 cgattgagag gatttgaatg actgacaaaa tgcaaagttt agctttagcc ccagttggca
    23881 acctagattc ctacatccgg gcagctaacg cgtggccgat gttgtcggct gacgaggagc
    23941 gggcgctggc tgaaaagctg cattaccatg gcgatctgga agcagctaaa acgctgatcc
    24001 tgtctcacct gcggtttgtt gttcatattg ctcgtaatta tgcgggctat ggcctgccac
    24061 aggcggattt gattcaggaa ggtaacatcg gcctgatgaa agcagtgcgc cgttttaacc
    24121 cggaagtggg tgtgcgcctg gtctccttcg ccgtccactg gatcaaagca gagatccacg
    24181 aatacgttct gcggaactgg cgtatcgtca aagttgcgac caccaaagcg cagcgcaaac
    24241 tgttcttcaa cctgcgtaaa accaagcagc gtctgggctg gtttaaccag gatgaagtcg
    24301 aaatggtggc ccgtgaactg ggcgtaacca gcaaagacgt acgtgagatg gaatcacgta
    24361 tggcggcaca ggacatgacc tttgacctgt cttccgacga cgattccgac agccagccga
    24421 tggcaccggt gctctatctg caggataaat catctaactt tgccgacggc atcgaagatg
    24481 ataactggga agagcaggcg gcaaaccgtc tgaccgacgc gatgcatggt ctggacgaac
    24541 gcagccagga catcatccgt gcgcgctggc tggacgaaga caacaagtcc acgttgcagg
    24601 aactggctga ccgttacggt gtttccgctg aacgtgtgcg ccaactggaa aagaacgcga
    24661 tgaaaaaact tcgcgccgct attgaagcgt aatttccgct attaagcaga gaaccctgga
    24721 tgagagtccg gggtttttgt tttttgggcc tctacaataa tcaattcccc ctccggcaaa
    24781 acgccaatcc ccacgcagat tgttaataaa ctgtcaaaat agctattcca atatcataaa
    24841 aatcgggtat gttttagcag agtatgctgc taaagcacgg gtagtcatgc ataaaacgaa
    24901 ataaagtgct gaacaacaac atcacaacac acgtaataac cagaagaatg gggattctca
    24961 ggatgaacat aaagggtaaa gcgttactgg caggatgtat cgcgctggca ttcagcaata
    25021 tggctctggc agaagatatt aaagtcgcgg tcgtgggtgc aatgtccggt ccggttgcgc
    25081 agtacggtga ccaggagttt accggcgcag agcaggcggt tgcggatatc aacgctaaag
    25141 gcggcattaa aggcaacaaa ctgcaaatcg taaaatatga cgatgcctgt gacccgaaac
    25201 aggcggttgc ggtggcgaac aaagtcgtta acgacggcat taaatatgtg attggtcacc
    25261 tctgttcttc atcaacgcag cctgcgtctg acatctacga agacgaaggc attttaatga
    25321 tcaccccagc ggcaaccgcg ccggagctga ccgcccgtgg ctatcagctg atcctgcgca
    25381 ccaccggcct ggactccgac caggggccga cggcggcgaa atatattctt gagaaagtga
    25441 aaccgcagcg tattgctatc gttcacgaca aacagcaata cggcgaaggt ctggcacgag
    25501 cggtgcagga cggcctgaag aaaggcaatg ctaacgtggt gttctttgat ggtatcaccg
    25561 ccggggaaaa agatttctca acgctggtgg cgcgtctgaa aaaagagaat atcgacttcg
    25621 tttactacgg cggttatcac ccggaaatgg ggcaaatcct gcgtcaggca cgcgcggcag
    25681 ggctgaaaac tggaaggtgt ggctaacgtt tcgctgtcta acattgcggg cgaatcagcg
    25741 gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcgaa caaacccatt
    25801 gttgatgcga tcaaagcgaa aaaacaggaa ccaagcggcg cattcgtctg gaccacctac
    25861 gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc tgaaatcgcc
    25921 aaatacctaa aagcgaactc cgtggatacc gtgatggggc cactgacctg ggatgagaaa
    25981 ggcgatctaa agggctttga gttcggcgta tttgactggc acgccaacgg cacggcgacc
    26041 gatgcgaagt aattgttaat cggcaacttt gggttgccgc caaattgcta atatcgaata
    26101 cgttgcttca tgccggatgc ggcgtaaacg ccttatccgg cctacaagat ccaaagaaat
    26161 cagtaaattg cagcacacat tataggcctg ataagcgtag cgcatcaggc aatttctcgt
    26221 tggtcatcac ttttgaaatc aacttgacga ttaacgcttc tcccagccgc cctgcagtgc
    26281 cgtaaatccc agtgcctgca taaacgccgt catcacaccg cgatcttcca cgcctgcatc
    26341 cgccatccac cagcatgaaa cgccaggatt gttacgcaaa acctcttcca gcagatattg
    26401 ccccacaccg cgacggcggg tgacttcccg cacgcgtaga gaatccagtg ctccctcggt
    26461 gccgcttaag gttacccgca cggccgcaag tagacgctcg ttaaaacgcg cggcgtagat
    26521 acggtggtta tcgtcaacct gtaaagagga aggggaatac tccggccaga tcttttgcag
    26581 gtcaatccgg tcctggtcgc taaagttttc taatcgaatg atggtcagct tcatgggtaa
    26641 cccgtgtaaa tcacaaaagt ggaaccagtg tagcgaaata atttaatcgg aggctttctc
    26701 ttttttattt cttttggcag gtgattaatt ttttaacagc aataattaca aagttaaaac
    26761 attagagaat gaaaaatgtc cagcataatc ccctgaatga tagtgaatta ttccgccctt
    26821 tgtgccgtta ttttatgctg acaaaggcac ttttttctgt ttatctatca ataaattcag
    26881 aatattatct gtttttaatc gactgaaaaa tagggatttt aatcgctatc atcacaaaat
    26941 actgcgctaa ccccttaatc agacaggcaa aaacagtgca gtataaaaaa agaacagtct
    27001 gatttgttaa cacataaaaa caaagcaaca caacatcacg aatggggatt tttgactatg
    27061 aaacggaatg cgaaaactat catcgcaggg atgattgcac tggcaatttc acacaccgct
    27121 atggctgacg atattaaagt cgccgttgtc ggcgcgatgt ccggcccgat tgcccagtgg
    27181 ggcgatatgg aatttaacgg cgcgcgtcag gcgattaaag acattaatgc caaaggggga
    27241 attaagggcg acaagctggt tggcgtggaa tatgacgacg cctgcgaccc gaaacaagcc
    27301 gttgcggtcg ccaacaaaat cgttaacgac ggcattaaat acgttattgg tcatctgtgt
    27361 tcttcctcta cccagccagc gtcggatatc tacgaagacg aaggtattct gatgatctcg
    27421 ccgggagcga ccaacccgga gctgacccaa cgcggttatc aacacattat gcgtactgcc
    27481 gggctggact cttcccaggg gcctacggcg gcaaaataca ttcttgagac ggtgaagccc
    27541 cagcgcatcg ccatcattca cgacaaacaa cagtatggcg aagggctggc gcgttcggtg
    27601 caggacgggc tgaaagcggc taacgccaac gtcgtcttct tcgacggtat taccgccggg
    27661 gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag aaaatatcga cttcgtttac
    27721 tacggcggtt actacccgga aatggggcag atgctgcgcc aggcccgttc cgttggcctg
    27781 aaaacccagt ttatggggcc ggaaggtgtc ggtaacgcat cgctgttgaa cattgccggt
    27841 gatgccgccg aaggcatgtt ggtcactatg ccaaaacgct atgaccagga tccggcaaac
    27901 cagggcatcg ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc ttatgtctgg
    27961 atcacttacg cggcagtgca atctctggca actgcccttg agcgtaccgg cagcgatgag
    28021 ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat tgggccgctg
    28081 aactgggatg aaaaaggcga tcttaaggga tttgattttg gtgtcttcca gtggcacgcc
    28141 gacggttcat ccacggcagc caagtgatca tcccaccgcc cgtaaaatgc gggcgggttt
    28201 agaaaggtta ccttatgtct gagcagtttt tgtatttctt gcagcagatg tttaacggcg
    28261 tcacgctggg cagtacctac gcgctgatag ccatcggcta caccatggtt tacggcatta
    28321 tcggcatgat caacttcgcc cacggcgagg tttatatgat tggcagctac gtctcattta
    28381 tgatcatcgc cgcgctgatg atgatgggca ttgataccgg ctggctgctg gtggcggcgg
    28441 gattcgtcgg cgcaatcgtc atcgccagcg cctacggctg gagtatcgaa cgggtagctt
    28501 accgcccggt gcgtaactct aagcgcctga ttgcgcttat ttccgcaatc ggtatgtcca
    28561 tcttcctgca aaactacgtc agcctgaccg aaggttcgcg cgacgtggcg ctgccgagcc
    28621 tgtttaacgg tcagtgggtg gtgggccata gcgaaaactt ctctgcctct attaccacca
    28681 tgcaggcggt gatctggatt gtcaccttcc tcgccatgct ggcgctgacg attttcattc
    28741 gctattcccg tatggggcgc gcctgtcgtg cctgcgcgga agatctgaaa atggcgagcc
    28801 tgcttggcat caacaccgac cgggtgattg ctttgacctt tgttattggc gcggcgatgg
    28861 cggcagtggc tggcgtactg ctcggtcagt tctactgcgt cattaacccc tatatcggct
    28921 ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg tgggattggc agcattccag
    28981 gggcgatgat tggcgggctg atcctgggga ttgcggaggc gctctcttct gcctatctga
    29041 gtacggaata taaagatgtg gtctcattcg ccctgctgat tctggtgctg ctggtgatgc
    29101 cgaccggtat tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc atattgcaat
    29161 ggcactgctc tctgccgcga tgttctttgt cctggcgggc gtctttatgg gcgtacaact
    29221 ggagctggat ggcaccaaac tggtggtcga cacggcttca gacatccgtt ggcagtggat
    29281 gtttatcggc acggcggtgg tctttttctt ccagcttttg cgaccggctt tccagaaagg
    29341 gttgaaaagc gtttccgggc cgaagtttat tctgcccgcg attgatgggt ccacggtgaa
    29401 gcaaaagctg ttcctcgtgg cgctgctggt gcttgtggtg gcgtggccgt ttatggtgtc
    29461 acgtgggacg gtggatatcg ccactctgac catgatctac attatcctcg gtctcgggct
    29521 gaatgtggtt gttggtcttt ctggtctgct ggtgctgggg tacggcggtt tttacgccat
    29581 cggcgcttac acttttgcgc tgctcaatca ctattacggt ctggggttct ggacctgttt
    29641 gccgattgcc ggactgatgg cagcggcggc gggtttcctg ctcggttttc cggtgctgcg
    29701 tctgcgcggt gactatctgg cgatcgttac ccttggtttc ggcgaaattg tgcgcatatt
    29761 gctgctcaat aacactgaaa tcaccggcgg cccgaacgga atcagtcaga tcccgaaacc
    29821 gacactcttc ggactcgagt tcagccgtac cgctcgtgaa ggcggctggg acacgttcag
    29881 taatttcttt ggcctgaaat acgatccgtc cgatcgcgtt atcttcctct acctggtggc
    29941 gttgctgctg gtggtgttaa gcctgtttgt cattaaccgc ctgctgcgga tgccgctggg
    30001 ccgcgcgtgg gaagcgttgc gtgaagacga gatcgcctgt cgttcgctgg gcttaagccc
    30061 gcgccgtatc aagctgaccg cttttaccat cagcgccgca tttgccggtt ttgccggaac
    30121 gctgtttgcg gcgcgtcagg gctttgtcag cccggaatcc ttcacctttg ctgaatcggc
    30181 ctttgtgctg gcgatagtgg tgctcggcgg tatgggctcg caatttgcag tgattctggc
    30241 ggctgttctg ctggtggtgt cgcgcgaatt gatgcgtgat ttcaacgaat acagcatgtt
    30301 aatgctcggt ggtttgatgg tgctgatgat gatctggcgt ccgcaggggt tgctgcccat
    30361 gacgcgcccg caactgaagc tgaaaaacgg cgcagcgaaa ggagagcagg catgagtcag
    30421 ccattattat ctgttaacgg tctgatgatg cgcttcggcg gcctgctggc ggtgaacaac
    30481 gtcaatcttg aactgtaccc gcaggaaatc gtctcgttaa tcggccctaa cggtgccgga
    30541 aaaaccacgg tttttaactg cctgaccggt ttctacaaac ccaccggcgg caccatttta
    30601 ctgcgcgatc agcatcttga aggtttaccg gggcagcaaa ttgcccgcat gggcgtggtg
    30661 cgcaccttcc agcatgtgcg tctgttccgt gaaatgacgg taattgaaaa cctgctggtg
    30721 gcgcagcatc agcaactgaa aaccgggctg ttctctggcc tgttgaaaac gccttctttc
    30781 cgtcgcgccc agagcgaagc gctcgaccgc gccgccacct ggcttgagcg cattggcttg
    30841 ctggaacacg ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg ccgtctggag
    30901 attgcccgct gcatggtgac gcagccggag attttaatgc tcgacgaacc ggcggcaggt
    30961 cttaacccaa aagaaaccaa agagctggat gagctgattg ccgaactgcg caatcatcac
    31021 aacaccacta tcttgttgat tgaacacgat atgaagctgg tgatggggat ttcagaccga
    31081 atttatgtgg tcaatcaggg gacgccactg gcaaacggta cgccggagca aatccgtaat
    31141 aacccggacg tgatccgtgc ctatttaggt gaggcataag atggaaaaag tcatgttgtc
    31201 ctttgacaaa gtcagcgccc actacggcaa aatccaggcg ctgcatgagg tgagcctgca
    31261 tatcaatcag ggcgagattg tcactctcat tggcgcgaac ggggcgggga aaaccacctt
    31321 gctcggcacg ttatgcggcg atccgcgcgc caccagcggg cgaattgtgt ttgatgataa
    31381 agacattacc gactggcaga cggcgaaaat catgcgcgaa gcggtagcga ttgtcccgga
    31441 agggcgtcgc gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg gcggcttctt
    31501 tgccgaacgt gaccagtttc aggagcgcat aaagtgggtg tatgaactgt ttccacgtct
    31561 gcatgagcgc cgtgttcagc gggcgggcac catgtccggc ggtgaacagc agatgctggc
    31621 gattggtcgc gcgctgatga gcaatccgcg tttgttgtta cttgatgagc catcgctcgg
    31681 tcttgcgccg attatcatcc agcaaatttt cgacaccatc gagcagctgc gcgagcaggg
    31741 gatgactatc ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg cggatcgcgg
    31801 ctacgtgctg gaaaacggcc atgtggtgct ctccgatacc ggcgacgcgc tgctggcgaa
    31861 cgaagcagtg agaagtgcgt atttaggcgg gtaataacac gttgattgat aaggagtcaa
    31921 aagactcctt tgagacaggt gacaaatgta aaattgcctg atgcgctgcg cttatcaggc
    31981 ctactgggtg agttgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttt
    32041 acgccgcatc cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga ccttctattt
    32101 ttccgcttat ccgtgcccca tctcccattt tccctcaccc acgccgtcac cgccttgtca
    32161 tctttctgac accttactat cttacaaatg taacaaaaaa gttatttttc tgtaattcga
    32221 gcatgtcatg ttaccccgcg agcataaaac gcgtgaattc gcgcattcgg tacaacaaga
    32281 gagataaacg atgaaaccgt tacattatac agcttcagca ctggcgctcg gactggcgtt
    32341 aatggggaat gcacaggcag tgacgaccat tccgttctgg cattctatgg aaggggaact
    32401 gggtaaagag gtggattctc tggcccaacg ttttaacgcc gaaaatccgg attacaaaat
    32461 tgtaccgacc tataaaggca actacgaaca gaatttaagc gcggggattg ccgcatttcg
    32521 taccggcaac gctccggcta ttttgcaggt ttatgaagtt ggcaccgcca ccatgatggc
    32581 gtcgaaagcc attaaaccgg tgtatgacgt gtttaaagag gcagggattc agttcgatga
    32641 gtcgcagttt gtgccgacgg tttccggtta ctactccgac agcaaaaccg gccacttact
    32701 ctcccagccg ttcaacagct cgactcccgt tctctattac aacaaagacg ccttcaagaa
    32761 agcggggtta gacccggaac agccgccgaa aacctggcag gatctggcgg actattccgc
    32821 aaaactgaaa gcctccggta ttaagtgcgg ctacgccagc ggctggcagg gctggatcca
    32881 actggaaaac tttagcgcct ggaacggtct gccgtttgcc agcaaaaaca acggctttga
    32941 cggcacggac gcggtgctgg agttcaacaa gccggagcag gtgaaacaca tcgccatgct
    33001 cgaggagatg aacaagaagg gcgacttcag ctacgtcggt cgtaaggatg aatccaccga
    33061 gaagttctat aacggtgatt gcgcgatgac caccgcctct tccggttctc ttgccaacat
    33121 tcgcgagtac gccaaattta actacggcgt aggtatgatg ccttacgacg ccgatgcgaa
    33181 agatgcgcca caaaacgcca ttatcggcgg agccagcctg tgggtgatgc agggcaaaga
    33241 taaagaaacg tataccggcg tggcgaagtt tctcgacttc ctcgcgaagc cagaaaacgc
    33301 tgctgagtgg catcagaaaa ccggttatct gccaattacc aaagcggcgt atgacctgac
    33361 ccgtgagcag ggcttttacg agaaaaaccc aggtgcggat accgcgacgc gtcagatgct
    33421 gaacaagccg ccgttgccgt tcaccaaagg gcttcgtctg ggcaacatgc cgcagatccg
    33481 cgtgattgtg gatgaagagc ttgagagcgt gtggaccggt aagaagacgc cacagcaggc
    33541 gctggatacc gcagttgagc gtgggaacca gttactgcgc cgctttgaga aatcgacgaa
    33601 gtcttaatca gtgtaatgcc ggatgcgttt cgcttatctg acctggcatc gcgtgtaggc
    33661 cggataagcg aagcgcatcc ggcacagttc aggaattaac cgtaatgtca tcatcccgtc
    33721 cggtgttccg ctcgcgctgg ctgccttatc tgctggtcgc gccgcagctc atcatcaccg
    33781 ttatcttttt tatctggcct gcgggcgaag cgttgtggta ctcgctacaa agcgtcgatc
    33841 cgtttggttt ctccagccag tttgtcggtc tggataactt cgtcacgctg tttcatgaca
    33901 gctattatct cgactccttc tggacgacga taaaattcag cacctttgtc accgtcagcg
    33961 gtttgctggt atcgctgttc tttgcggcgc tggtggagta catcgtgcgc ggcagtcgtt
    34021 tctaccaaac cttaatgttg ctgccgtatg ccgtggctcc cgccgttgcc gccgtattgt
    34081 ggatcttcct gtttaacccc ggtcgcgggc tgatcaccca ttttctcgct gagttcggct
    34141 acgactggaa ccacgcgcaa aatagcggtc aggcgatgtt tctggtggtg tttgcctcgg
    34201 tgtggaagca aatcagctac aactttctgt tcttctatgc cgcgctgcaa tccattcccc
    34261 gttcgttgat cgaagccgca gccatcgacg gtgcagggcc gattcgccgc ttctttaaga
    34321 ttgcgctgcc gcttatcgcc ccggtgagtt tcttcctgct ggtagtgaac caggtgtatg
    34381 ccttcttcga caccttcccg gtgatcgacg ccgccacgtc cggcgggccg gttcaggcca
    34441 ccacgacgct gatttataag atctaccgcg aaggttttac cggactggat ctggcttcgt
    34501 ctgccgcaca gtcggtggtg ttgatgttcc tcgtcatcgt gctgacggtg gtgcagttcc
    34561 gctatgttga aagcaaggtg cgttaccaat gattgagaac cgtccgtggc tgacgatatt
    34621 cagccatacc atgctgatcc tcgggatcgc ggtgatcctc ttcccgctgt acgtggcgtt
    34681 tgtcgcggcg acgctggata aacaggccgt ctacgccgcg ccgatgacgc tcatccccgg
    34741 cacgcatctg ctggaaaaca tccacaacat ctgggtgaac ggggtaggca caaatagcgc
    34801 gccgttctgg cggatgttgc ttaacagctt tgtgatggcg ttcagcatta cgctcggcaa
    34861 aattactgtc tcgatgctct cggcatttgc cattgtctgg tttcgttttc cgctacgtaa
    34921 cctcttcttc tggatgattt ttatcaccct gatgctgccg gttgaagtac gtatcttccc
    34981 gacggtggaa gtcatcgcca acctgaagat gctcgacagc tacgccggtt taacgctgcc
    35041 gctgatggcc tcggcgaccg ccacgtttct gtttcgccag ttctttatga cgctgccgga
    35101 tgaactggtg gaagcggcgc ggatcgacgg tgcgtcacca atgcgcttct tttgcgacat
    35161 cgtttttccg ctctccaaaa ctaatctggc ggcgctgttt gtgatcacct ttatttacgg
    35221 ctggaaccag tatttgtggc cgttgttgat tattaccgac gtggatctcg gcaccaccgt
    35281 ggcagggatc aaagggatga tcgccactgg cgaaggcacc acggaatgga actcagtgat
    35341 ggcggcgatg ttgttaaccc tgatcccacc agtagtgatt gttttagtga tgcagcgtgc
    35401 cttcgtgcgc ggcctggtcg atagtgagaa ataagatggc aggactgaaa ttacaggcag
    35461 taaccaaaag ctgggatggc aaaacctagg ccattaaacc gctgaccctt gatgtggcgg
    35521 atggcgaatt tatcgtgatg gtcgggccgt ctggctgcgg gaaatcgacg ctgctgcgta
    35581 tggttgccgg gctggagcgg gtgaccgaag gcgatatctg gatcaacgac cagcgcgtga
    35641 ccgaaatgga gccgaaagat cgcgggattg cgatggtgtt ccagaattac gcgctttatc
    35701 cgcatatgaa cgtcgaagaa aacatggcgt gggggctgaa aattcgcggc atgggcaagc
    35761 agcaaatagc cgagcgcgtt aaagaagcgg cgcgcattct ggagctggac ggtctgctca
    35821 aacgtcgccc gcgcgagctt tccggcggtc agcgccagcg tgtggcgatg ggacgcgcga
    35881 ttgtgcgcga tccggcggtg ttcttgtttg atgagccact ctctaacctc gatgccaagc
    35941 tgcgcgtaca gatgcgtctt gaactgcaac agctgcaccg tcgcctgaaa acgacttcac
    36001 tctacgttac tcacgatcag gttgaggcga tgacgctcgc ccagcgagta atggtgatga
    36061 acggcggcgt tgccgaacag attggcacgc cggtggaagt ctacgaaaag cccgccagcc
    36121 tgtttgtggc gagttttatc ggcagcccgg cgatgaacct gctgacaggc cgcatgaata
    36181 atgaaggcac gcatttcgaa ctggacggcg gtatcgtgct accgctaaac ggtggctacc
    36241 gtcagtatgc cgggcgtaaa atgaccctcg gcattcgccc ggaacatatt gcgctaagct
    36301 cgcaagcaga agctggtggt gcgactggcg catcaggagc gcctgacggc aggcagcacg
    36361 ctgtggctgc atctggcgga aaatcagctg catctttttg atggtgaaac aggacaacga
    36421 gtatgagtaa ctggccttat ccccgcatcg tcgctcatcg tggcggcggt aagctggccc
    36481 cggaaaacac cctggcggca atcgacgtcg gggcaaaata cggtcataag atgatcgaat
    36541 ttgacgcgaa gttatcgaaa gatggcgaga tcttcctgct ccatgacgac aatctcgaac
    36601 gcaccagcaa cggctggggc gtcgcgggtg aactgaactg gcaggattta ctgcgcgtgg
    36661 atgcgggcag ttggtacagc aaagcgttta aaggtgagcc gctgccgttg ctttcgcagg
    36721 tggcggaacg ctgtcgcgaa cacgggatga tggcgaatat cgaaatcaaa cccaccactg
    36781 gcactggacc attaacgggc aaaatggtgg cgctggcggc acgccaactg tgggccggta
    36841 tgacgccgcc gctgctgtca tcgtttgaga ttgatgcttt agaagctgca caacaggcgg
    36901 caccggaact gccgcgtggt ttattgcttg atgagtggcg cgacgactgg cgcgaactga
    36961 ccgcgcggct gggctgcgtc tctattcatc tcaatcataa gttactcgat aaagcgcgag
    37021 tgatgcagtt gaaagacgcc ggattacgga ttctggttta taccgtcaat aaaccccagc
    37081 gcgcggcaga gttgctgcgc tggggcgtgg attgcatctg taccgatgcg attgacgtga
    37141 ttggtccgaa ctttacggcc caatagtttt caacggaatg tcaggctgcg gcgtgctggg
    37201 ctgattcagc atgtcgccgt ttctcctctc cggcagcata tgctgctgat tcaacgaact
    37261 atccggatta cggttgctgt ttaacatccc gccgttggtg ttgggcagca tttgctgccg
    37321 ggcgggattt cgctcccccg gctgtgactg caacacccgc tgagaattat tgtttatctg
    37381 gttttctaaa tgctgctgtt gcaactgagt ttgcgttttc agttgctgat tcagcatccc
    37441 tttttgctgg atttgctgag tctgcatctg ggtttgcatc cgctgctggc tgggtatctg
    37501 ataccccggc tggttagggt tgttcagagt attaatgggc tgtgcaaagc cgacaaacgg
    37561 caggagtgcc gtaagaatca gaagtcgttt catcgcgtat cctcctctga agatatcctt
    37621 taagtttact cgcttcccga caaaacgatg attaattcag agttatatac caggcttagc
    37681 tggggttgcc ccttaatctc tggagaataa cgatgataaa accgacgttt ttacgccggg
    37741 tggccattgc tgctctgctc tcaggaagtt gttttagcgc cgccgccgcg cctcctgcgc
    37801 cgcccgtctc gtatggtgtg gaggaagatg tcttccaccc ggtacgcgcg aaacagggaa
    37861 tggtagcgtc tgtggacgcc actgccactc aggtgggggt ggatatcctc aaggagggcg
    37921 ggaatgccgt tgatgccgcc gtggcggtgg gctacgcgct ggcggtaacg catccgcagg
    37981 cagggaatct gggcggtggt ggttttatgt taatccgctc gaaaaatggc aataccacgg
    38041 ctatcgattt ccgcgaaatg gcacccgcca aagcgacccg cgatatgttc ctcgatgatc
    38101 agggcaaccc ggacagcaaa aaatcactca cttcgcatct ggcttccggc acaccgggta
    38161 cggtagcagg tttctcgctg gcgctggata aatacggcac catgccgctg aacaaagtcg
    38221 tgcagcccgc gtttaaactg gcacgcgatg gttttatcgt taacgacgcg ctggctgacg
    38281 atctcaaaac ctacggtagc gaagtgttgc cgaatcacga aaacagtaaa gctatcttct
    38341 ggaaagaggg cgagccgctg aaaaagggcg acacgctggt gcaggcgaac ctggcaaaga
    38401 gcctggagat gattgctgaa aacggcccgg acgaattcta taaaggcacg attgcggaac
    38461 agatcgccca ggagatgcag aaaaacggtg gcttgatcac taaagaagat ttagcggcct
    38521 ataaagcggt cgaacgcact ccgataagcg gcgattatcg cgggtatcag gtttactcca
    38581 tgccaccgcc atcctccggc gggatccata tcgtacaaat cctcaatatt ctggaaaact
    38641 tcgatatgaa gaaatacggc tttggcagcg ccgatgcgat gcaaatcatg gcagaagcgg
    38701 agaaatacgc ctacgccgac cgctcggaat atcttggcga cccggatttt gtcaaagtcc
    38761 cgtggcaggc actgaccaat aaagcctatg ccaaatccat tgccgatcaa attgatatca
    38821 acaaagcgaa gccgtccagt gagattcgcc ctggcaagct tgcgccttat gagagtaatc
    38881 aaactaccca ttactctgta gtggataaag acggtaacgc ggtggcggtg acctatacgc
    38941 tgaacaccac cttcggtacg ggtattgtcg cgggcgagag cggtattctg cttaataacc
    39001 agatggatga tttctccgcc aaaccgggcg taccgaacgt ttacgggctg gtgggcggtg
    39061 atgccaacgc cgtcgggccg aacaaacgcc cgctgtcgtc gatgtcgccg accattgtgg
    39121 tgaaagacgg taaaacctgg ctggttaccg gtagcccagg cggtagccgg attattacca
    39181 cggtgctgca aatggtggtg aatagcatcg attatggcat gaacgtcgcc gaagcgacca
    39241 atgcgccgcg tttccaccat cagtggttgc cggacgagct gcgtgtcgaa aaagggttta
    39301 gcccggatac gctcaagctg ctggaagcaa aaggtcagaa agtggcgctg aaagaggcga
    39361 tgggcagcac acaaagcatt atggttgggc cggacggtga gttgtacggc gcatccgacc
    39421 cgcgctcggt ggatgattta acggcggggt attaatatct taagccctcc cggtaggtga
    39481 gggcttaaat gacaaactta gattgcaaat tctctatttc acccgcgcca tataatacgc
    39541 atcgacatat tcaccattac gcaatgcgta cttcttaccg gtcccttcaa tctcaaagcc
    39601 gaatttttta tagaccttaa ttgctggcgc gttatcgaca aacacggtta gttcaatgcg
    39661 atctacccgt aaccagttgt cgcacatttc aatcatctct cgcatcaggg cgctggcgac
    39721 gccgcggttc tt
//
LOCUS       DBKEKI010000025        36765 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.025, whole genome shotgun sequence.
ACCESSION   DBKEKI010000025 DBKEKI010000000
VERSION     DBKEKI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.025"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..854)
                     /locus_tag="ACUQPX_001247"
                     /pseudo
     CDS             complement(1..854)
                     /locus_tag="ACUQPX_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
     gene            1092..1912
                     /locus_tag="ACUQPX_001248"
                     /pseudo
     CDS             1092..1912
                     /locus_tag="ACUQPX_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            complement(2208..2810)
                     /locus_tag="ACUQPX_001249"
     CDS             complement(2208..2810)
                     /locus_tag="ACUQPX_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HHV9056009.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICHATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELVRYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKSSKGIRIPATKKSLPEINSVQNN"
     gene            3133..3516
                     /gene="traM"
                     /locus_tag="ACUQPX_001250"
     CDS             3133..3516
                     /gene="traM"
                     /locus_tag="ACUQPX_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HHV9056010.1"
                     /translation="MARVILYISNDVYDKVNAIVEQRRQEGARDKDISLSGTASMLLE
                     LGLRVYEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDDE"
     gene            3707..4360
                     /locus_tag="ACUQPX_001251"
     CDS             3707..4360
                     /locus_tag="ACUQPX_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HHV9056011.1"
                     /translation="MCAMDRRERALIRQLHFPNDLRENISLLKYPVCIRFEDGSFIGT
                     NHSFERFIKLSYCSCDEWFEHLTLESKLQLSRSEMDSYFSTYGVSYINNVLISAMSWS
                     MVIETLNTNEGCFFIWRFIESNDKNISTSLISKSNDGLMFFSGEYVDIEPYLVGFSHY
                     YSAAVTKISISKSKKKTMNFFKMNGFSSRDHWLDEMLRSEKILFLYDRVKGILKNKC"
     gene            4485..4712
                     /gene="traY"
                     /locus_tag="ACUQPX_001252"
     CDS             4485..4712
                     /gene="traY"
                     /locus_tag="ACUQPX_001252"
                     /inference="COORDINATES: protein motif:HMM:NF010300.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HHV9056012.1"
                     /translation="MSRNIIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVR
                     LRDHLNRFPDFYNLDAIKEGAEETDSIIKDL"
     gene            4745..5107
                     /gene="traA"
                     /locus_tag="ACUQPX_001253"
     CDS             4745..5107
                     /gene="traA"
                     /locus_tag="ACUQPX_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HHV9056013.1"
                     /translation="MNTVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAQGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVGLK"
     gene            5112..5423
                     /gene="traL"
                     /locus_tag="ACUQPX_001254"
     CDS             5112..5423
                     /gene="traL"
                     /locus_tag="ACUQPX_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HHV9056014.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFALPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSYWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            5445..6011
                     /gene="traE"
                     /locus_tag="ACUQPX_001255"
     CDS             5445..6011
                     /gene="traE"
                     /locus_tag="ACUQPX_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HHV9056015.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            5998..6726
                     /gene="traK"
                     /locus_tag="ACUQPX_001256"
     CDS             5998..6726
                     /gene="traK"
                     /locus_tag="ACUQPX_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HHV9056016.1"
                     /translation="MRKNNTAIIFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            6726..8153
                     /gene="traB"
                     /locus_tag="ACUQPX_001257"
     CDS             6726..8153
                     /gene="traB"
                     /locus_tag="ACUQPX_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HHV9056017.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGALYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKNGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEENQESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            8143..8727
                     /gene="traP"
                     /locus_tag="ACUQPX_001258"
     CDS             8143..8727
                     /gene="traP"
                     /locus_tag="ACUQPX_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HHV9056018.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGQLLVQEIDFVRQTAPAGQFPVRECLPSLHDLPGAEEKICRYRAADA
                     ADYVRETDRSLMQLVTALWATLALMYVSLAVISGKYPVLPGKMKCIRGVTADERLTEV
                     YTEDASLPEKIRKCPVYFPDDRTNRNNGDKNEHA"
     gene            8714..9034
                     /locus_tag="ACUQPX_001259"
     CDS             8714..9034
                     /locus_tag="ACUQPX_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HHV9056019.1"
                     /translation="MNMRNINVITARSVPDKSVSDDFMHAVLSNCTTRIVLPAPKEFS
                     SESLPHNFNMAAVGVMKKGMSMSLFSSPGHIVVAGQSGGGKSVPVKELIDRIIQKYKE
                     PFDE"
     gene            9027..9278
                     /gene="trbG"
                     /locus_tag="ACUQPX_001260"
     CDS             9027..9278
                     /gene="trbG"
                     /locus_tag="ACUQPX_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HHV9056020.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFVNCDEKGEGQ"
     gene            9275..9790
                     /gene="traV"
                     /locus_tag="ACUQPX_001261"
     CDS             9275..9790
                     /gene="traV"
                     /locus_tag="ACUQPX_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HHV9056021.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTITATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            9925..10146
                     /gene="traR"
                     /locus_tag="ACUQPX_001262"
     CDS             9925..10146
                     /gene="traR"
                     /locus_tag="ACUQPX_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HHV9056022.1"
                     /translation="MSDEVDEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            10139..10615
                     /locus_tag="ACUQPX_001263"
     CDS             10139..10615
                     /locus_tag="ACUQPX_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056023.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            10695..10913
                     /locus_tag="ACUQPX_001264"
     CDS             10695..10913
                     /locus_tag="ACUQPX_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056024.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNSVMETLNGGGQ"
     gene            10941..11288
                     /locus_tag="ACUQPX_001265"
     CDS             10941..11288
                     /locus_tag="ACUQPX_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056025.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            11414..14044
                     /gene="traC"
                     /locus_tag="ACUQPX_001266"
     CDS             11414..14044
                     /gene="traC"
                     /locus_tag="ACUQPX_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HHV9056026.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKKKQARIDDVVDFLKNARDNDQYVESPTIRSRLDEMI
                     VLLDQYTANGTYGRYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGMSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            14041..14427
                     /gene="trbI"
                     /locus_tag="ACUQPX_001267"
     CDS             14041..14427
                     /gene="trbI"
                     /locus_tag="ACUQPX_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HHV9056027.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAISYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            14424..15056
                     /gene="traW"
                     /locus_tag="ACUQPX_001268"
     CDS             14424..15056
                     /gene="traW"
                     /locus_tag="ACUQPX_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HHV9056028.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            15053..16045
                     /gene="traU"
                     /locus_tag="ACUQPX_001269"
     CDS             15053..16045
                     /gene="traU"
                     /locus_tag="ACUQPX_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HHV9056029.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            16075..16380
                     /locus_tag="ACUQPX_001270"
     CDS             16075..16380
                     /locus_tag="ACUQPX_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056030.1"
                     /translation="MATMDSKYIQHLRKIGAVLLRLISRTLKLFPFGLVIVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCVFMAVFLNYLFSDEFMPVNSRK"
     gene            16389..17027
                     /gene="trbC"
                     /locus_tag="ACUQPX_001271"
     CDS             16389..17027
                     /gene="trbC"
                     /locus_tag="ACUQPX_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HHV9056031.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTTENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLSETRHYGIPATLRGMVNNDLKATADAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            17024..18832
                     /gene="traN"
                     /locus_tag="ACUQPX_001272"
     CDS             17024..18832
                     /gene="traN"
                     /locus_tag="ACUQPX_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HHV9056032.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQS
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSMTSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFRRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMLCGGDVFCLDGECDKAQSGKSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGRAKHPDCRGITVDELQKIQFDRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            18859..19116
                     /locus_tag="ACUQPX_001273"
     CDS             18859..19116
                     /locus_tag="ACUQPX_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HHV9056033.1"
                     /translation="MKVIFTSNRLVDFLIRLLITAIVISPVIIWSWDTVKETTADEML
                     AAAFVILYSGVLLFILYFCFSALTGLQKADERKNDERNEDE"
     gene            19109..19852
                     /gene="traF"
                     /locus_tag="ACUQPX_001274"
     CDS             19109..19852
                     /gene="traF"
                     /locus_tag="ACUQPX_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HHV9056034.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKISPKEKENKPVPAA
                     PRQELDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            19868..20206
                     /gene="trbA"
                     /locus_tag="ACUQPX_001275"
     CDS             19868..20206
                     /gene="trbA"
                     /locus_tag="ACUQPX_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HHV9056035.1"
                     /translation="MSEDYLKMFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMNQGAAMVVPMAIGSLPNVTSFDRFRRIFICIMLSSAWS
                     GVMWFFIRGL"
     gene            20333..20617
                     /gene="traQ"
                     /locus_tag="ACUQPX_001276"
     CDS             20333..20617
                     /gene="traQ"
                     /locus_tag="ACUQPX_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HHV9056036.1"
                     /translation="MISKCRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            20604..21149
                     /gene="trbB"
                     /locus_tag="ACUQPX_001277"
     CDS             20604..21149
                     /gene="trbB"
                     /locus_tag="ACUQPX_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HHV9056037.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPSDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            21079..21420
                     /gene="trbJ"
                     /locus_tag="ACUQPX_001278"
     CDS             21079..21420
                     /gene="trbJ"
                     /locus_tag="ACUQPX_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HHV9056038.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSYYSRITCLNESSEI
                     SEETYNERKKK"
     gene            21401..21793
                     /gene="trbF"
                     /locus_tag="ACUQPX_001279"
     CDS             21401..21793
                     /gene="trbF"
                     /locus_tag="ACUQPX_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HHV9056039.1"
                     /translation="MKEKRNDAELKNRKTKRNYDYERRVSDIYFDLFFVFVAAGTFLW
                     VIMHSIFDACIDSWKADPALNNFRYMWNILMYVIPYTLWAFAGGFLIVYVRNPLNELI
                     NGGIRIFRLKRRMRRENSFREGNNDASH"
     gene            21780..23153
                     /gene="traH"
                     /locus_tag="ACUQPX_001280"
     CDS             21780..23153
                     /gene="traH"
                     /locus_tag="ACUQPX_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HHV9056040.1"
                     /translation="MPRIKPLLVLCAVLLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMSYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            23150..25975
                     /gene="traG"
                     /locus_tag="ACUQPX_001281"
     CDS             23150..25975
                     /gene="traG"
                     /locus_tag="ACUQPX_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HHV9056041.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMNTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTSTRLSGGMY
                     ADAHAEWGVKPKILGVGGGVGIRGGFKGSLDATDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEVAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGYTQNSIHGEENIVDRQYSELKNNHKQEEIQQNNRYNEENKR
                     QKLMPTPSEDALKQMVDDKKERLKGPL"
     gene            25972..26481
                     /gene="traS"
                     /locus_tag="ACUQPX_001282"
     CDS             25972..26481
                     /gene="traS"
                     /locus_tag="ACUQPX_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HHV9056042.1"
                     /translation="MITHYDIKMEMQKLKEVLSVEGVNIPSLLQVIKPGTYVFLWVLL
                     WPTFLRLVSVKSDVRDVGFDICASGMMGFLLFVAITNGMMLYLAIPDSFRKDSKIINF
                     MYSKSKTYILLFLIVFSMVSFMHSILYVFALMITFILFFLVYTIDINRYNLSAIASVI
                     GLFKKESVS"
     gene            26495..27226
                     /gene="traT"
                     /locus_tag="ACUQPX_001283"
     CDS             26495..27226
                     /gene="traT"
                     /locus_tag="ACUQPX_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HHV9056043.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPDSERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            27429..28166
                     /locus_tag="ACUQPX_001284"
     CDS             27429..28166
                     /locus_tag="ACUQPX_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056044.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNSLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQGENITWSLSSQS
                     PADKYDLINKTSRVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRAAGGYHHRHTGYTWSVSVN"
     gene            28217..30415
                     /gene="traD"
                     /locus_tag="ACUQPX_001285"
     CDS             28217..30415
                     /gene="traD"
                     /locus_tag="ACUQPX_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HHV9056045.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLI
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPQQPQQPVSPAINDKKSDSGVNIPAGGIEQELKMKPEEEMEQQLPPGIS
                     ESGEVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            30415..35685
                     /gene="traI"
                     /locus_tag="ACUQPX_001286"
     CDS             30415..35685
                     /gene="traI"
                     /locus_tag="ACUQPX_001286"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HHV9056046.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRTEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWEKNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLSGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSEDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            35705..36451
                     /gene="traX"
                     /locus_tag="ACUQPX_001287"
     CDS             35705..36451
                     /gene="traX"
                     /locus_tag="ACUQPX_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HHV9056047.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHANIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            36506..>36765
                     /gene="finO"
                     /locus_tag="ACUQPX_001288"
     CDS             36506..>36765
                     /gene="finO"
                     /locus_tag="ACUQPX_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HHV9056048.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPG"
BASE COUNT         9243 a         8514 c        10199 g         8809 t
ORIGIN      
        1 cctcttacgc cgccacagcg ccatctgcag ggcatcgcag gccagttgcg ccgtcatgcg
       61 tggcgacatt gaccagccaa taacggcacg tgaccacagg tcaatgacca ctgccagata
      121 cagccagcct tcatctgtac gtaagtacgt gatgtctcct gcccacttct ggttcgggcc
      181 actggcgtaa aaatcctgct ccaacagatt ttctgacaca ggcaggccgt gtgcgcggta
      241 gctgaccggg ctgaacttcc gggaggcctt tgccctcagt ccctgacggc gcaggcttgc
      301 cgccacggtt tttacgttaa aggggtaacc ctgagcacgc agttcatccg tcaggcgtgg
      361 ggcaccgtaa cgctgttttg accgggtaaa agccgcgagg acaacgctgt cgcagtgttg
      421 gcggaactgc tgacgcgtgc ttatccttgt ccgccgctga caccacgtat accagccgct
      481 gcgggccacc cggagcacgc ggcacattgc tttgatgctg aactcagcct gatgtttttc
      541 aataaagaca tacttcattt caggcgcttc gcgaagtatg tcgcggcctt ttggaggata
      601 gccagctctt catcccgttc tgccagctgg cgtttgagac gtgcaatctc ggtagacatc
      661 tccagttcac gttcagaaga cgtctgctga ttttgctgtt tactgcgcca gttgtagagc
      721 tgtgattcat acaggctgag ttcacgggct gcggcagtaa caccgatgcg ttcagcaagc
      781 ttcagggctt cactgcgaaa ttcaggcgaa tgctgtttac ggggtttttt actggttgat
      841 actgtttttg tcatgtgagt cacctctgac tgagagttta ctcacttagc cgcgtgtcca
      901 ctattgctgg gtaagatcac agttcctgcg tcagattaag cgtgccggcc tcgatatccg
      961 ggcgatctgc tgaccggatg tgatgaatat ccctcctgat tgttgcaatc ttgttaatgg
     1021 agatcggggg ggatgatatt aagcggaaaa attttttatt aaatttatcc tttcataaag
     1081 gagtagctgt tatgcgatta gcttcccgtt ttggtcggta taattccatc cgtcgtgaac
     1141 gtcctttaac ggatgatgaa ttaatgcagt tcgtaccttc ggtattttcc ggtgataaac
     1201 atgagtcccg gagtgaacgt tatacgtata ttccaacaat caatatcatc aataagttac
     1261 gtgatgaagg tttccagcca ttctttgcct gccagagtcg ggttcgtgat ttgggacgtc
     1321 gcgaatacag taaacatatg ttacgtcttc gcagggaagg gcatattaac gggcaggaag
     1381 ttcctgaaat tatcctactt aattcacatg atggttcatc cagttatcag atgatccccg
     1441 ggatttttcg ttttgtctgc acaaatggcc tggtgtgcgg aaataatttt ggcgaaatcc
     1501 gcgttccaca taaaggtgat attgttgggc aggttatcga gggagcgtat gaagtactcg
     1561 gtgtctttga taaggtcact gataatatgg agcgatgaaa gaaattcatc ttaacagtga
     1621 tgagcaacat ttatttggca gaactgcact gatagtcagg tatgaagatg aaaataaaac
     1681 gccagtgacg cctgaacaaa taattacccc ccgtcgtcgg gaggataaac agaatgatct
     1741 ctggacaacc tggcagcggg ttcaggagaa tatgataaaa ggtggattat cggggcgaag
     1801 tacctccggt aaaaatacca ggacaagagc cattacaggt attgatggtg atatccgaat
     1861 caacaaggct ttatggatga ttgccgaaca gttcagaaag tggatgtcgt gataatataa
     1921 gtaaagcccc gaaaattttt cggggcttta cttatattat cattcttcgg catcgctgtg
     1981 tcgtctgata tacctcaatt tcatcaactg gatttccggt tgtctgtaaa tattgtgccc
     2041 gcgaaaaatg gtctggcagg aggctgcgca gttaccgggt atatatcaga tgggttcccg
     2101 cttcgcggag cttcggccag tcagtattgt cactcatatg atatttttgt gtgtggcctt
     2161 ccatgccgct tttgcggcat aaccggtatc tgacaatgtc tgtaagatta attgttctgc
     2221 acgctgttaa tttccggaag tgattttttc gtggccggaa tccggatacc tttactgctt
     2281 tttatcccgg tataaatccg gtaaacatct gatgcgtagg caagacgtct ctggttctgg
     2341 cgttcggtct tcctgaatcc ggcattgtat gcaccaacgg cctcccagga gacgccccat
     2401 tttttaaagg ctattgccag ataataagca ccggtataaa tgttcatgca gggatctgtt
     2461 gtcagatgtt ccggcttaat tccatagcgg accagttcgt taaaatgctg ggaatctacc
     2521 tgcatcagtc cgctgccata tcccgttacc ggattaatac cgatggcatt aacccggtaa
     2581 cgggattctt tccatgatat tgctctcagt aaatcaggat ctattttgta atcccggcct
     2641 gcaagatcaa agcaatcagt ggcatggcag atctcattta taaacatcag gcagatggct
     2701 aacatccatt ttttcatttt tccacctctg gtgactttat ccgtaaataa tttaacccac
     2761 tccacaaaaa ggctcaacag gttggtggtt ctcaccacca aaagcaccac accccacgca
     2821 aaaacaagtt tttgctgatt tgcgttttaa atcatgtaat taacaaatgc tatttatatt
     2881 taataattat gaattattaa ataaagagtc gttggcgatc ctgttacgac cctatagcga
     2941 cccttattag gatcagtact gacaattttc atgaaaaaca ttgatatata ttaggggcac
     3001 ccctagcgcc ggatcgcatt tttttatagg atgatcctag aggcgctgct aggagtgtct
     3061 ttcttacttg taaacactat attcatattt atagtgaaaa gttcattgct taatgatgaa
     3121 aggttttatt ttatggccag agtaattttg tatatcagta atgatgtcta tgataaagta
     3181 aatgcgatag ttgaacagcg tcgtcaggag ggggcaagag ataaagatat aagcctttca
     3241 ggaactgctt caatgcttct tgaactgggg cttcgcgtat atgaggctca gatggagcgt
     3301 aaagagtctg catttaatca gactgagttt aataaattac ttcttgaatg tgttgtaaaa
     3361 acacagtcat cagtagcgaa aattttgggt attgagtctc taagtcctca tgtctccggg
     3421 aatccaaagt ttgaatatgc caatatggtt gaagatattc gtgagaaggt gtcatctgag
     3481 atggaacgat tttttccaaa aaatgatgac gaataaaaaa gaattaaaag ttgatttact
     3541 tcaaataaca gagttttgat ctggttcaat ttgatgacaa ttaaccacat aaaggttata
     3601 ttaattatgt ggttaatgcc acgttaaaaa tttagtttga atcgtcaatg cagggagtag
     3661 cccctccctg cattgactgt ccatagaatc ctttgaggag gttcctatgt gtgcaatgga
     3721 tcgtagagaa agagcactca ttcgtcaatt acatttccct aacgacttaa gagagaacat
     3781 ttcattattg aaatatcccg tatgtatacg ttttgaagac ggttctttta taggaacaaa
     3841 tcacagcttc gagcgattta ttaaattatc atattgttca tgtgatgaat ggtttgagca
     3901 tttgacttta gaatccaagt tacaactatc aagatctgag atggattcat atttttcaac
     3961 ttatggtgta agttatatta ataatgtttt aattagtgct atgtcatgga gcatggttat
     4021 cgaaacactc aatacaaacg aaggttgttt tttcatctgg cgatttattg agtcaaatga
     4081 caaaaatatt tcaacatctt tgatttctaa atctaatgat gggttgatgt ttttttctgg
     4141 ggaatatgta gatattgaac cctatcttgt agggtttagc cattattatt ctgcagcagt
     4201 gacaaaaatt agtatatcta aatccaagaa gaaaacgatg aattttttca aaatgaatgg
     4261 gtttagttcc agagatcact ggttggatga aatgctcagg tctgaaaaaa tactatttct
     4321 atatgataga gtaaaaggaa tattaaaaaa caagtgttaa tttattgtga acttaaggga
     4381 accatcggtt cccttttttg ttaatataat ttacgttatt tttgtttcta tttttatgga
     4441 tccgtataat tatatttaat tatctaagtg gctgaggagg agacttgagc agaaatataa
     4501 taaggcctgc tccgggcaat aaagttttac tggtattgga cgatgctact aatcacaagc
     4561 tcttaggtgc ccgggaacgt agtgggagaa caaaaactaa tgaggtgcta gtcaggttac
     4621 gtgatcatct gaaccgtttt cctgactttt ataatctgga tgcaataaag gagggagcag
     4681 aggaaactga ttcgataatt aaagatcttt aggtttatta atctatccac aatagaggta
     4741 acttatgaat actgttttaa gtgttcaggg tgcttctgcg cccgtcaaaa agaaatcgtt
     4801 tttttctaaa ttcactcgtc tgaatatgct tcgcctggct cgcgctgtga tccctgctgc
     4861 tgttctgatg atgttcttcc cgcaactggc aatggcagca cagggccagg acctgatggc
     4921 aagcggtaac accacggtta aggcgacctt cggtaaggac tccagtgttg ttaaatgggt
     4981 tgttctggct gaagttctgg tcggtgcggt catgtacatg atgaccaaaa acgtcaagtt
     5041 cctggccggt tttgccatca tctctgtatt tattgctgtg ggtatggccg tcgttggcct
     5101 gaaataaaat gatgtcggga gacgagaata aacttaagaa atatcgtttc ccggaaacac
     5161 tgaccaacca gagccgctgg tttgccctgc cactggatga actgatcccc gccgcaatct
     5221 gtattggctg gggtatcaca acatcgaaat atctgttcgg tattggtgca gcggttctgg
     5281 tttatttcgg gattaaaaaa ctgaaaaaag ggcggggcag ttactggtta cgtgacctga
     5341 tttactggta tatgccaaca gccctgctgc gcggtatttt tcataatgtt cccgattcgt
     5401 gtttccggca gtggattaaa tagaactgat accaggattg ttatatggaa cacggtgccc
     5461 gtttaagtac cagtcgtgta atggccatcg cctttatatt tatgtcagtg cttattgttc
     5521 tcagcctctc tgttaacgtc attcaggggg tgaataacta ccgtcttcag aatgagcaac
     5581 gcactgccgt gaccccaatg ggatttaatg tgccctttgc cgtgtcacag aacagtgccg
     5641 acgcctctta tttacagcag atggcgctgt catttattgc cctccgtctg aatgtttcat
     5701 cagaaaccgt cgatgcctca catcaggcgc ttctgcaata tatccgcccg ggcgcacaga
     5761 accagatgaa agttattctg gctgaagaag cgaagcgtat taaaaacgat aacgtgaact
     5821 cagccttttt ccagaccagt gtccgtgtat ggcctcagta tggccgtgtg gaaattcgtg
     5881 gtgtgcttaa aacctggatt ggtgattcaa aacctttcac tgatatcaaa cattacatcc
     5941 ttattctgaa gcgggaaaac ggggtgacct ggctggataa tttcggggaa acagacgatg
     6001 agaaaaaata atacggcaat aatattcggc agcctgtttt tttcctgcag cgtgatggcc
     6061 gcaaacggta cgctggcccc caccgtggtg ccaatggtga acggtggtca ggccagtatt
     6121 gccatcagca ataccagccc gaatctgttt accgttcccg gtgaccggat tatcgccgtg
     6181 aacagtctgg atggtgccct gaccaataat gagcagaccg cctccggcgg tgtggtggtg
     6241 gccaccgtca acaaaaagcc ctttacgttc attctggaaa cagaacgtgg tctgaacctt
     6301 tccattcagg ccgttccccg tgaaggtgcg gggcgtacca ttcagctggt cagtgacctg
     6361 cgcggaaccg gagaagaagc cggcgcgtgg gaaacgtcca cgccttacga atccctgctt
     6421 gtgaccatca gccaggctgt ccgtggcgga aaattacctg caggctggta tcaggttcct
     6481 gtgacaaagg aaaccctgca ggccccggcg gggctgtctt cagtggcaga tgccgtatgg
     6541 acggggaatc acctgaagat ggtccgcttt gccgtggaaa ataaaacgct gtctgccctg
     6601 aatatccggg aaagtgattt ctggcagccg ggaacccgtg ccgtgatgtt cagccagcct
     6661 gccagccagt tactggcagg tgcgcgcatg gatgtgtatg tcatccgtga cggggagggc
     6721 aactgatggc cagtatcaat accattgtga aacgcaagca gtacctgtgg ctggggattg
     6781 tggttgtcgg tgcagcctcc gcgattggtg gtgcactgta tctgtctgat gtggacatgt
     6841 ccggtaacgg tgaggcggtg gctgaacagg agcctgtgcc ggatatgacc ggtgtggtgg
     6901 atacgacctt tgatgacaag gtgcgtcagc atgccaccac agagatgcag gtgacggcag
     6961 cgcagatgca gaagcagtat gaggaaatcc gtcgtgaact ggatgtcctg aacaaacagc
     7021 gcggtgatga ccagcgtcgt attgaaaagc tgggacagga taatgctgcc ctggcagagc
     7081 aggtaaaagc cctgggtgca aatcccgtca ccgcgaccgg tgagcctgta ccgcagacgc
     7141 cagcctcacc gcccgggccg gaaggcgaac cacagccagg aaacaccccc gtatccttcc
     7201 cgccgcaggg cagcgttgct gttccaccgc cgacggcgtt ttatcccggg aatggtgtca
     7261 cgccaccacc acaggtgacg taccagtctg tgccggtgcc taaccggata cagcgtaagg
     7321 tgtttacccg taatgaggga aaacagggac catcgctgcc gtacattccg tcaggaagtt
     7381 ttgcgaaagc catgctgatt gaaggggcgg atgccaatgc ctcagtcacc ggtaatgaat
     7441 ccacggtgcc gatgcagctg cgtatcaccg gtctggtgga aatgccgaac agcaagacgt
     7501 atgacgcaac gggatgtttt gtggggctgg aagcctgggg ggatgtctcc agtgaacgtg
     7561 ctattgttcg cacccgtaat atcagctgcc tgaagaatgg caaaaccatt gatatgccga
     7621 ttaaggggca tgtcagcttc cggggtaaaa acggtatcaa gggcgaagtg gtgatgcgta
     7681 acggcaaaat cctcggctgg gcatggggcg cgggatttgt tgacggtatc ggtcagggaa
     7741 tggagcgcgc ctcccagccg gctgtcgggc tgggtgccac agccgcttac ggggctggtg
     7801 atgtcctgaa aatggggatc ggtggcggcg catcgaaagc cgcacagacg ctcagtgact
     7861 actacatcaa acgtgccgaa cagtatcacc cggtgatacc gattggtgcg ggcaacgaag
     7921 tgaccgtggt gttccaggac ggcttccagc tgaaaaccgt ggaagagatg gcgctggaac
     7981 gcacgcagag cagagcggaa gaagagaatc aggaaagtcc ggttcctgtt ccgccatcgg
     8041 ctgaaagtca tcttaacggc tttaatactg accagatgct gaagcagctg ggcaacctga
     8101 atccgcagca gtttatgtcc ggcagccagg gagggggcaa cgatggcaaa taatatgtcg
     8161 tcccgtcagg cgtgccatgc cgcccggtat gttgtcgccc gggttctgcg aggactgttc
     8221 tggtgtctga agtacaccgt cattctgcca ctggccacaa tggccctgat ggctctgttt
     8281 gttttatgga aggacaacac cacgccaggg cagttactgg ttcaggaaat tgacttcgta
     8341 agacaaacag cccctgccgg gcagtttcct gtaagagagt gtttgccttc tctgcatgat
     8401 cttccaggag cagaagagaa aatctgccgt taccgtgctg ctgatgctgc agattatgtc
     8461 agggagactg accggtcact gatgcaactg gttaccgcat tatgggcaac actggcattg
     8521 atgtatgtct cactggcagt tatctccggg aaatatccgg tgctgcccgg aaaaatgaaa
     8581 tgtatccggg gggtaaccgc agatgaacgt ctgacggaag tttatacgga agacgcttcc
     8641 ctgccggaaa aaatcaggaa atgccctgtt tattttcctg atgacagaac aaacagaaac
     8701 aacggagata aaaatgaaca tgcgtaatat taatgttatt actgcccgct ctgtaccgga
     8761 taaaagcgta tcagatgatt ttatgcatgc tgttctcagt aactgcacca caagaatcgt
     8821 acttcctgca ccgaaggaat tcagttctga atcattgccg cacaatttta atatggctgc
     8881 tgtcggtgtg atgaagaagg gaatgtcgat gagcttattt tcttctccgg ggcatattgt
     8941 ggttgccgga caatccggtg gcggaaaatc tgttccggtg aaagaactaa tcgacagaat
     9001 tattcagaaa tataaggaac ccttcgatga ataagctggt ttctgatggc agcgtcaaaa
     9061 aaataaatta tccggtgctg tatgaatccg gaataacacc tccgttatgt gaagtcagtg
     9121 caccagaacc tgatgcgggg gggaaacgca ttgtggcata tgtttataaa tcatcacgga
     9181 atacggtttt tgaaaatcct gacattgtaa aaacctgtac ggtcagggat ttgaagaatg
     9241 attttgtgaa ctgcgatgag aaaggagagg gacaatgaaa cagatttctt tatttattcc
     9301 tctgctgggg accttattac tttccggctg tgccggaacc agtacggaat ttgagtgtaa
     9361 cgccaccaca tccgatacct gtatgacgat ggagcaggcc aatgagaagg ccaaaaaact
     9421 ggagcagtcc tcagaagcaa agccggttgc ggcatcactg ccgcgcctgg ctgaagggaa
     9481 cttccggaca atgccggtgc agaccatcac cgcgaccact ccgtccggca gcaggcctgc
     9541 tgtgacagca catccggagc agaaactgct ggcaccacgc ccgctgttta ccgctgcccg
     9601 ggaagtgaaa acggttgttc cggtcagttc agttacgccg gtaacacctc cccgtccgct
     9661 aagaacgggt gagcagacgg ctgcattatg gatagcgcct tatattgata atcaggatgt
     9721 ttatcatcag ccatccagtg tgttttttgt tattaaaccg tctgcgtggg gaaaaccacg
     9781 tattaattaa ctggccctga agttgtatat cagccggcaa tgccggcggg ctttctgtat
     9841 tcaaaatctg cctgtaaaac gagtgccctg tgcgtgaaaa gggatgggcg gtagcgtgtc
     9901 agaacatgtc gggaggaata ttccgtgagt gatgaagtcg atgaagcata ttcagtaaca
     9961 gaacaactga ccatgacagg aataaaccgg atacgccaga aaataaatgc tcatggtatt
    10021 cctgtttatc tctgtgaagc atgcggtaat cctattccgg aagcccggcg gaaaatattt
    10081 cccggtgtga cgttgtgcgt tgaatgtcag gcgtatcagg aaaggcagag gaaacactat
    10141 gcataaatca gaaaatgaaa aaatagattt tattctggat gagcatgagc ggatatccag
    10201 aaaacagggt tctttgtgta tggcactggg taagggcttt ttagttatag ctattctttg
    10261 tggtgttgcc acattctgtg tttctggcct gtatctgaaa tccctgtgcc tgtccgttgc
    10321 ctgctgttgt ggggtattca gtcgtatttt taattattac agcgtaccgg aggaatcacg
    10381 catggtactc agtcgtcagg aattactccg gcttatgcgt ctgactgaag acgtaccaga
    10441 tgtgagacag gaactgctta accgtttgtt gtccggtaaa aaactgactg gtcgggatga
    10501 acaggatatc cggcgtctct ggcaggagaa agtggatgca atgcaggagt ctgccacccg
    10561 tcagcgtgaa caggatacta ttcggaagtt tactgaaggg aataaatccg aatgagaatc
    10621 tgcagtgttt tatgacctca taagttaact gaagggatat tcagtgatta atatacaagc
    10681 ggagataata aagaatgcat acctttattt ttcttgtgct gataaaaaat attaacggtg
    10741 gcggaagctg gaatattaca cccatgccaa atacggacgt atgcgtgcag gttcttcgga
    10801 caatcgaagc acatggcggc aggggctttg gaaatacagg ggagtgtatt gaagttaaaa
    10861 caaatgaacc tttgaacagc gtgatggaaa cactaaatgg tggtgggcaa tgagtgctta
    10921 ctgcttcgaa ggaaaatgaa atgaagtcga tatatttaaa atctgttctg gcttttatat
    10981 ttgttggcgt tatggctatg attgtatgta ttccttttta catcgtttac cttgcgcaac
    11041 aacctgcaac accagaacaa ctgacagaga ttttacagga aactccctgt gcagcagaag
    11101 catttcagga gacacttaat tatcagtctg aacctttaac acttggtaag gcaaataaaa
    11161 tcgcatcaga atgcagaaaa agaaacgaaa tggcagaggt gaaacgggta agagaaaatg
    11221 agcgcaataa gattcgcgaa aaacaaattc aggcattaaa tgatgctcat tcagtaaagg
    11281 aacgttaatc ctggtttcct gttaaaagcc tccatctctt atttgtgctg tggtttcagg
    11341 aatggcactg cgtgatatat ccacggccac agactgatat tttccttttt taaacgcact
    11401 aaggaatact catatgagta ataacccact tgaggccgtc actcaggcgg ttaactccct
    11461 cgtcacagca ctgaaactgc ctgacgaatc agcaaaggcc aatgaagtgc tgggcgaaat
    11521 gagcttcccg cagttcagcc gtctgctgcc gtaccgtgat tacaaccagg agtccggcct
    11581 gttcatgaat gacaccacga tgggctttat gctggaagcc attcccatca atggtgcgaa
    11641 tgagtccatt gtggaggcgc tcgatcatat gctgcgcacc aaactgccgc gcggtattcc
    11701 gttgtgtatc catctgatgt ccagtcagct ggttggtgac aggattgaat acgggctgcg
    11761 tgagttctcc tggtcaggtg aacaggctga acggtttaac gccattaccc gtgcctatta
    11821 tatgaaagcg gcagcgacac agttcccgct gccggagggg atgaatctgc ccctgaccct
    11881 gcgccattac cgggtgttta tctcgtactg ttctccctcg aagaaaaaaa gccgggccga
    11941 cattctggaa atggaaaatc tggtgaaaat catccgtgcc tcttttcacg gcgcaaaaat
    12001 caccacacag acagtggatg cacaggcttt catcgagatt gtcggggaga tgattaacca
    12061 taatccggac tctctgtacc cgaaaagacg tcagctggac ccgtattctg atctgaatta
    12121 tcagtgtgtg gaggacagtt ttgatctgaa ggtccgggcg gattacctga cgctggggct
    12181 gcgtgagaac ggcaggaaca gcacagcccg tatcctgaat ttccatctgg cccgtaaccc
    12241 ggaaatcgcc ttcctgtgga acatggccga taactacagc aatctgctga acccggaaat
    12301 gtccatttcc tgcccgttca tcctgacgct gacactggtg gtggaagatc aggtgaaaac
    12361 ccacagtgaa gccaacctga agtacatgga cctggagaaa aagtcgaaga cctcctatgc
    12421 caaatggttt ccgtccgtgg agaaagaagc taaggagtgg ggggaactgc gccagcggct
    12481 gggctccggt cagtcttctg tcgtgtccta cttccttaac atcacagcat tctgcaagga
    12541 caacaatgaa acggcgctgg aagtggagca ggacattctg aacagctttc gtaaaaacgg
    12601 ttttgagctg atttcaccgc gctttaacca catgcgcaat ttcctgacct gcctgccctt
    12661 tatggccggg aaagggctgt ttaaacagct gaaagaggcc ggtgtggttc agcgagcaga
    12721 gagctttaat gtggccaacc tgatgccgtt agtggcggat aaccccctga caccggcagg
    12781 cctgctggca cccacctacc gtaaccagct ggcgtttatc gatatcttct ttaaggggat
    12841 gaataacacc aactacaaca tggcggtctg tggaacatcc ggggccggta aaaccgggtt
    12901 gatacagcca cttatccgca gcgtgctgga ctccgggggc tttgccgtgg tgttcgacat
    12961 gggggatggc tacaagtctt tgtgtgagaa catggggggc gtatatctgg acggtgaaac
    13021 cctgcgcttt aacccgtttg cgaacatcac tgatattgac cagtcagcgg agcgtgtccg
    13081 tgaccagttg tcggtgatgg ccagccccaa cggtaacctg gatgaagtgc atgaaggtct
    13141 gttgctgcag gcggtcagag cctcctggct ggctaaaaag aagcaggcgc gcattgatga
    13201 tgtggtggat ttcctgaaaa acgcccgtga taacgaccag tatgtcgagt caccgaccat
    13261 ccgcagccgt ctggacgaaa tgattgtgct gcttgaccag tacactgcca acggcactta
    13321 cggccggtat tttaactctg atgagccgtc cctgcgggat gacgcaaaaa tggtggtgct
    13381 ggagctgggc ggactggaag accgtccgtc actgctggtt gcggtgatgt tcagccttat
    13441 catttacatc gagaaccgga tgtaccgcac gccgcgtacc ctcaagaaac tgaacgtcat
    13501 tgatgaaggc tggcgtctgc tggacttcaa aaatcgtaaa gttggtgagt ttattcaaaa
    13561 aggttaccgc acctgtcgcc gtcataccgg tgcctatatc accatcacac agaacatcgt
    13621 cgactttgac tctgacaagg catccagcgc agcccgtgcg gcatggggta actcctccta
    13681 caaaattatc ctcaaacaga gtgcgaagga gttcgcgaaa tataaccagt tattcccgga
    13741 tcagttccag ccactgcagc gcgacatgat tggtaagttt ggtgcggcca aagaccagtg
    13801 gttcagttcc ttcctgctgc aggtggaaaa ccattcctcc tggcaccgtc tgtttgtgga
    13861 cccgttaagc cgtgccatgt acagctctga cggcccggat tttgagtttg tgcagcagaa
    13921 acgcagagag ggtatgagta ttcatgaggc ggtgtggcag ctggcgtgga agaaatcggg
    13981 gccggagatg gcttcgctgg aggcctggct ggaagaacat gagaaataca ggagtgttgc
    14041 atgaccacaa cgcaaaaaac gactgacgtt acagcccccc gacgcagcca ctggtggtgg
    14101 accgtgccgg gatgcctggc aatggtgtta ctgaacgcag ccatcagtta tggcattgtc
    14161 agactgaacg caccggtgac tgccgccttc aacatgaaac agaccgtgga tgcgtttttt
    14221 gacagcgcca gtcagaaaca actgtcagaa gcacagtcaa aagccctttc agcgcgtttt
    14281 aacacggcac tggaagccag cctgcaggca tggcagcaga aacaccacgc ggtcattctg
    14341 gtgtcgcctg ccgtggtaca gggggcaccg gatatcaccc gggaaatcca gcaggatatt
    14401 gcccggcgaa tgagggcgga accatgagat gccgggggct gattgccctg ctgatatggg
    14461 ggcagagtgt ggccgccgcc gatcttggca cctggggcga tctgtggccg gtaaaggagc
    14521 cggacatgct gacggtgatc atgcagcgcc tgacggcact ggagcagtcc ggtgagatgg
    14581 gccggaaaat ggatgccttt aaggagcggg tgatccgtaa cagtctgcgg ccccctgccg
    14641 tgcccggtat cggacgcaca gagaaatacg gcagccggtt gtttgacccg tccgtcagac
    14701 tggctgcgga tatccgggat aacgaagggc gggtgtttgc ccgtcagggt gaagtgatga
    14761 atccgctgca gtatgttcct tttaaccaga cgctgtattt catcaatggc gatgatccgg
    14821 cgcaggtggc ctggatgaaa cgccagacgc cgcccacact ggagagcaaa attatcctcg
    14881 tgcagggcag tatcccggag atgcagaagt ctctggacag ccgtgtttac tttgaccaga
    14941 acggtgtgct ctgtcagcgc ctcggcattg atcaggtccc ggcacgggtg agcgccgttc
    15001 ccggcgatcg cttcctgaag gtggaattta ttccggcaga ggagggcaga aaatgaagcg
    15061 aagcctgtgg ctgctgatgt tattccttct cgccggtcat gtccctgcag cgtctgcgga
    15121 ttctgcctgt gaggggcgtt ttgtaaaccc gatcacggat atctgctgga gctgtatttt
    15181 cccgctctcg ctgggcagta tcaaagtcag tcagggtaag gtccccgaca cggcgaaccc
    15241 gtcgatgccc attcagattt gtccggcacc gccgccgctg ttcaggcgta tcgggctggc
    15301 cattggttac tgggagccga tggcgctgac ggacgtcacc cggtcaccgg gatgcatggt
    15361 gaacctgggc ttcagcctgc cggcttttgg taaaacggca cagggaacgg cgaaaaagga
    15421 tgagaagcag gtgaacgggg cgttctatca cgttcactgg tacaaatacc cgctgacgta
    15481 ctggctgaac atcatcacat cgctgggctg tctggaaggt ggtgacctgg atatcgctta
    15541 tctttctgaa atcgacccca cctggacgga cagcagcctt accaccattc tcaacccgga
    15601 agccgtcatt tttgccaatc cgatagcaca gggggcctgc gcagcagatg ccattgccag
    15661 tgcctttaat atgcctctcg atgttctgtt ctggtgtgcc ggttcgcagg gcagtatgta
    15721 cccgttcaat ggctgggtga gtaatgagtc cagtccgttg cagtcctccc tgctggtcag
    15781 tgaacgcatg gcattcaagc tgcaccgtca gggcatgatt atggaaacca tcgggaaaaa
    15841 taacgccgtc tgtaatgaat atccgtcccc aatcctgccc aaagaacgct ggcgttatca
    15901 gatggtgaat atgtatccgg acagcggaca gtgccatccg ttcgggcgca gtgtgatgcg
    15961 ctgggagacg ggaaaaaatc cgcccaacac aaagaaaaac ttcggctacc tgatgtggcg
    16021 taaacgtaac tgtgtattcc tgtaaatcat tttaatgaat aagaggaaat gaaaatggca
    16081 acaatggaca gtaaatatat tcagcacctc aggaaaatag gtgctgtgct gctcaggctg
    16141 ataagcagaa cattaaaatt atttcctttt ggtcttgtaa tagtgcccgt tatattattt
    16201 tattttgcac ctgacagttt tctcgctgaa attattcatg aatggaagaa ggcagatata
    16261 tcagaacaag tgacacttgt cagattatat ctgaaggtga catttacatg tgtgtttatg
    16321 gctgtttttc tcaattatct tttttctgat gagttcatgc ctgtaaatag cagaaagtga
    16381 gaaatatcat gaaccggaaa atatttgcac tttttgctct gacagcaggg atgcatcttg
    16441 ctgttcatgc ttcagaaaat gtgaacacaa ctgaaaaccg ccagttcctg aagcagcagg
    16501 aaaatttaag cagacaactg cgagagaaac ctgaccatca gctgaaagcc tgggcggaga
    16561 aacaggtgct ggaaaacccc cttcagcgtt cagataacca ttttctggat gagctggttc
    16621 gtaaacagca ggcttcgcag gacgggaaac cccggcaggg tgccctgtat tttgtgtcgt
    16681 tttccatacc cgaagagggg ctgaaaagga tgctgagcga aacccggcac tacggtattc
    16741 ccgccacgct gcggggcatg gtgaacaacg acctgaaggc cacggctgac gccgtgttgt
    16801 ccctggtgaa agacggcgca actgatggtg ttcagatcga cccgacgctg ttttcacagt
    16861 acggcattcg cagcgttccg gccctggtgg tgttctgcag ccaggggcac gacatcatcc
    16921 ggggaaatct gcgcgtcagg caggcgctgg agaaagtggc cgccacgggt gactgccggc
    16981 aggtggctca tgatttactg gcagggaaag gagattccgg gaaatgaaac gtattttacc
    17041 tctgatactg gctctggttg ccggcatggc acaggctgac agcaacagtg attaccgggc
    17101 cggttctgat tttgcccgtc agattcaggg gcagggtaca ggcagtattc agagctttaa
    17161 gccacaggag agtatccccg gctataacgc gaatccggac gagacaaaat attacggtgg
    17221 cgtgactgcc ggcggggatg gcggcctgaa aaatgacggc accacggaat gggcgaccgg
    17281 tgaaaccgga aaaaccatca cagagtcctt catgaacaag ccgaaagaca ttctgtcacc
    17341 ggatgcaccg ttcattcaga ccggcaggga tgtggtgaac cgggctgaca gcattgtggg
    17401 aaacaccggg cagcagtgca gtgcgcagga gattagcagg agtgaataca cgaattacac
    17461 ctgtgagcgg gatttacagg tggagcagta ctgcacacga actgcccgta tggagctgca
    17521 ggggagtacc acatgggaaa cccggacgct ggagtatgaa atgagtcagc tacctgcacg
    17581 tgaagtgaat ggtcagtatg ttgtctccat gacctctccc gttaccggtg aaattgtcga
    17641 tgcgcattac agctggagtc gtacttacct gcagaagagt gtacctatga caattacagt
    17701 actgggaacc ccactgagct ggaatgccaa atactcagca gatgcctcat ttacaccagt
    17761 acagaaaaca ctgactgccg gtgtggcttt tacgtcatct caccccgtcc gggtcgggaa
    17821 tacaaaattc agacgtcata cggcaatgaa gctgcgtctg gttgtcaggg taaaaaaagc
    17881 ctcgtacacc ccgtatgttg tctggtctga aagctgcccg ttcagcaagg agctggggaa
    17941 actgacaaaa acagaatgta cggaggctgg cgggaacagg acgctggtga aagacggtca
    18001 gtcatacagc atgtaccaga gctgctgggc ataccgggac acgtatgtca cacagtcagc
    18061 agacaaggga acctgtcaga cctacaccga caatccggcc tgtaccctgg tgtcacacca
    18121 gtgcgccttt tactccgaag aaggtgcctg tctgcatgaa tatgccacgt actcctgtga
    18181 gtcaaaaaca tccgggaaag tgatgctctg tgggggagat gtcttctgcc tcgatggcga
    18241 atgcgataag gctcagagcg ggaagagtaa tgattttgcc gaagctgttt ctcagctggc
    18301 agcactggcc gctgcaggta aggatgttgc ggcgctgaac ggggtggatg tccgtgcttt
    18361 caccggtcag gcgaaatttt gtaagaaggc tgcagccgga tacagcaact gctgtaagga
    18421 cagcggctgg ggacaggata tcgggctggc caaatgcagc agtgatgaaa aagccctggc
    18481 taaagcaaaa tcaaacaaac tgaccgtcag cgtcggggag ttctgttcga agaaagtgct
    18541 gggtgtctgt ctggagaaaa aacgcagcta ctgtcagttt gattcgaagc tggcgcagat
    18601 agtccagcag caggggcgca acggtcagct gcgtatcagt tttggccgtg cgaagcatcc
    18661 tgactgtcgg gggattacgg ttgatgagct gcagaaaatt cagttcgaca gactggattt
    18721 cactaacttc tacgaagacc tgatgaataa ccagaaaatc ccggacagtg gcgttctgac
    18781 gcagaaagtg aaagagcaga ttgctgacca gctgaaacag gcaggacagt aagaaatgga
    18841 aaataaccgg gggaaatgat gaaagtcata ttcacttcta atcgtcttgt tgattttctt
    18901 atccggcttt tgattacagc gattgtcatt tctccggtaa ttatctggtc gtgggatacg
    18961 gtaaaagaaa ccacggcaga cgagatgctt gccgctgctt tcgttatcct ttactccggt
    19021 gtgctcctgt ttattctgta tttttgtttt tctgccctga ctggcctgca gaaagctgat
    19081 gaaagaaaaa atgatgaaag gaatgaagat gaataaagca ttactgccac tgttactctg
    19141 ctgctttatt tttccggcgt cagggaaaga tgcaggctgg cagtggtata acgagaaaat
    19201 aagtccaaag gaaaaagaaa ataaacctgt acctgcagcc ccccgtcagg agctggatat
    19261 tatgcagaaa ctggccgcgc tgcagacggc aacaaagcgg gcgctgtacg aagccattct
    19321 gtatcccggc gtggataatt ttgtgaaata tttccggctg cagaattact ggactcagca
    19381 ggccgggctt ttcaccatga gtgctaaaaa ggccatgctg gcacatcccg aactggacta
    19441 taacctgcag tacagccatt acaacggcac ggtacggaac cagctggcgg cagaccaggc
    19501 gcagcagcga caggccattg cgaaactggc tgaacactac ggcatcatgt ttttttaccg
    19561 ggggcaggac cccatcgacg ggcagctggc gcaggtcatt aatggcttcc gggatacgta
    19621 tggtctgagt gttattcccg tttccgtgga tggcgtgatt aatccgctgt tgccggattc
    19681 ccggactgac caggggcagg cgcagcgcct cggcgtgaaa tatttcccgg ccatgatgct
    19741 ggttgacccg aaacagggca gtgttcgccc gttatcatac ggctttattt cgcaggacga
    19801 cctggcaaaa caattcctga acgtttctga agattttaaa ccaaattttt aatcgcggat
    19861 ttgatttatg agtgaagatt atttgaaaat gtttacaggt gttgttctgt taatatttgt
    19921 cattattgcc ggttatttct tttctgagcg taatgacagg aaaatgtttc tcctgagttc
    19981 actggttttc cttgttatta atatcgcgtg tttatatgtg cttaccgcca gtctctggtt
    20041 tctgtgtggt gcaattatga atcagggcgc agcaatggtt gttccaatgg ctattggttc
    20101 attaccgaac gttacgagct tcgacaggtt cagaagaata tttatctgta ttatgttgtc
    20161 atcggcatgg tccggagtga tgtggttttt tataaggggc ttatgacagg ctaagtcata
    20221 tataacggta ataaaaacca tacaaaataa tgaggttgat ctgattaata tcacacaaac
    20281 gcgaatatta cagacatgaa tattctcatg taaataataa ccggagttta acatgataag
    20341 taaatgcaga ttctctttac cccggcttga tattacgggt atgtgggtat tttccctggg
    20401 tgtctggttt catatcgtcg cccgtcttgt ttacagcaaa ccctggatgg ctttttttct
    20461 ggcggagttg attgcggcca tccttgtgct gttcggcgca taccaggtgc tggatgcatg
    20521 gattgcacgt gtcagcctgg aagagcgtga ggcactggaa gccaggcaac aggccatgat
    20581 ggaagggcag caggagggcg gacatgtctc tcactaaatt actgccggtc attttgttga
    20641 tgatggccac aggtgtgcag gcctccaccc gggatgaaat tgaacgactc tggaatccgc
    20701 agggtatggc cgtgcagcct gcacaaccgg cagcagacac atcagccaga acagaaaagc
    20761 cggctccccg ctggttccgc ctcagcaacg gcaggcaggt taacctggcc gactggaagg
    20821 tggtgctgtt tatgcagggg cattgccctt actgtcacca gtttgacccg gtactgaaac
    20881 agctggcaca gcagtacggc ttttctgttt ttccctacac cctggatggt cagggggata
    20941 ctgcctttcc ggaagcatta ccggtgccat cggacgtgat gcagaccttc ttcccgaata
    21001 tcccggtggc cacaccgacc actttcctgg tcaacgtcaa cacacttgag gcattaccgc
    21061 ttttgcaggg ggcaacggat gccgccagtt ttatggcgcg gatggatacc gttctgcaga
    21121 tatacggaga ggaaaaaggt gcgaaataag caggtggtgt tactcattgc cggaatatcc
    21181 ggcatcgcga cgggaataat cgtcagcctg aatatcccct ttatccgtca ggggcttttt
    21241 tatcccgcca gccctgtgga aattgtcgtt tcgctgtgtc ttaccttttc tgtttctgtt
    21301 gtgttttttg tgggggcaat tgtgggatgg atttcggtgt catattacag ccgcataacc
    21361 tgtctgaatg aatcatcaga aatatcagag gaaacttata atgaaagaaa aaagaaatga
    21421 tgcggaacta aaaaacagaa aaactaaacg gaattatgat tatgaacgca gggtttctga
    21481 tatatatttt gatctgtttt ttgtgttcgt tgctgccgga acttttttat gggtgattat
    21541 gcacagtatt tttgatgcct gtattgattc gtggaaagct gacccagcat taaataattt
    21601 tcgttatatg tggaacatcc tgatgtatgt cattccgtat acgttatggg cgtttgcagg
    21661 tggatttctt atcgtatacg tcaggaaccc tttaaatgaa ttaataaacg gggggatcag
    21721 gatattccgg ctgaaacgcc gtatgcgccg ggagaacagt ttcagggagg ggaataacga
    21781 tgcctcgcat taaacctctt ctggttcttt gtgccgtttt actgacggtc acacccgcag
    21841 cgtcagcgga tgtgaacagc gacatgaatc agttctttaa caagctgggc ttcgcctcca
    21901 ataccacaca gccgggcgtc tggcaggggc aggccgccgg gtatgcctat ggtggctccc
    21961 tgtatgcccg cacccaggtc aaaaatatcc agctgatttc catgacgctg ccggatatca
    22021 atgccggatg tggcggcatc gacgcctatc tcggctcgtt cagttttatt aatggcgatc
    22081 agttgcagcg ttttgttaag cagattatga gcaatgctgc cggttacttt tttgaccttg
    22141 ccctgcagac aacggtgccg gaaataaaaa ccgcaaaaga cttcctgcag aaaatggcaa
    22201 gtgacattaa cagtatgaac ctcagttcct gtcaggtggc acaggggctt gtgggggggc
    22261 tgttccctca gacgcaggaa tctcagcaga aagtttgtca ggacattggc agtgaaagca
    22321 atatcttcgc cgactgggcc gcatctcgtc aggggtgcac ggtcggaggg cagtcctcat
    22381 ctgttcgtga caaagccagc gacaaggaca aggagcgggt gctgaaaaac cagaatatca
    22441 tatgggaggc gctgaaaaaa aacagaatgt ttgacggcaa caaagagttg aaagaattta
    22501 tcatgacgct caccggtacg ctgatttttg gggaagacag cgagattacc cccctgccgg
    22561 cgcgtaccac tgaccgcgat atcctccggg cgattatgga ggggggaacg gcaaaaattt
    22621 accactgcaa cgattcggac aagtgtctga aggtggtggc ggatgcaaat gtgaccatcg
    22681 cccgggataa agccctgaag tcacagatta caaaactgct caccagtatt cagaataaag
    22741 cagtgagtga cactccgctt gacgcccggg aacaggggtt tatttccagc accaccattc
    22801 ccgtttttaa atatctgatt gacccgcaga tgctcggtgt gtccaacagc gtcgtttacc
    22861 agctgacgga ctatatcggc tacgacatca tgctccagta cattcaggaa ctgcttcagc
    22921 aggcccgggc catgattgcc accgggaact atccgcaggc tgtgatggac aatgttctgg
    22981 agaatctgaa tcaggcacag caacagattg ccattttcca gagtcaggta caggtccagc
    23041 aggatgcgct gctggttgtc gaacgtcaga tgagctacat gcgtcagcag ctttccgccc
    23101 gtatgctcag ccgttaccag aacaactatc acttcggagg gaattcactg tgaatgaagt
    23161 ttatgtgatt gccggtggtg agtggttgcg taacaacctg aatgccattg cagcatttat
    23221 gaatacccgg acctgggatt caattgaaaa aatcgcgctc accctgtctg ttgttgctgt
    23281 ggcttttatg tgggtacagc ggcataacgt gatggatttg ctgggctggg tggccgtgtt
    23341 tgtgcttatc agcctgctgg ttaatgtccg tacatcggtg cagattattg ataacagtga
    23401 cctggtcaaa gtccatcggg tggataatgt gccggtcggt ctggcgatgc cactttcact
    23461 gacgacacgt atcgggcatg caatggtggt cagctacgag atgatattca cccaaccgga
    23521 cagcgtcacc tacagcaaga caggcatgct gttcggggcg aatctgattg tgaaaagcac
    23581 cgatttcctg tcccggaatc cggaaatcat caatctgttc caggactacg tccagaactg
    23641 tgtgctgggt gatatttacc tgaatcataa atacacgctg gaagatctga tggcctccgc
    23701 tgacccctac acgctgattt tttcccgacc cagcccgcta cggggcgttt atgacaacaa
    23761 taataatttc ataacctgta aggatgcatc ggtcacgctg aaagacaggc tgaatctcga
    23821 tacaaagacg ggaggcaaga cctggcatta ttatgtgcag cagatatttg gcggcagacc
    23881 ggacccggac ctgttattca gacaactggt cagtgacagt tacagttatt tctacggctc
    23941 cagtcagtct gccagccaga ttatgcgcca gaacgtcaca ataaatgccc tgaaagaggg
    24001 tatcaccagt aatgctgccc gtaatggtga caccgccagc ctggtgaatc tggccaccac
    24061 gtcatcgatg gagaagcaac gtctggcgca tgtctctatc ggtcatgtga caatgcgaaa
    24121 cctgccaatg gtacagacta tcctgacggg aattgccatt ggtattttcc cgcttctggt
    24181 actggcagct gtcttcaaca aactgacact gtccgtcctg aaaggctatg tgtttgccct
    24241 gatgtggctg cagacctggc cgttgttgta tgccattctg aacagtgcca tgacattcta
    24301 tgcgaagatg aatggtgcgc cggtcgtgct gtctgaactc tctcagatac agctgaaata
    24361 ctctgacctg gcctctactg ccgggtatct ttcagccatg atccccccgt tgtcatggat
    24421 gatggtaaaa ggccttggcg caggtttttc cagcgtgtac agccacttcg cctcttcctc
    24481 tatcagtccg acggccagtg cagctggcag tgtggttgac ggtaattact cctacggcaa
    24541 catgcagacg gaaaacgtga acggcttcag ctggagcacc aacagcacca cgtcgtttgg
    24601 tcagatgatg taccagaccg gcagtggcgc aactgccaca cagacccgtg acggtaatat
    24661 ggtgatggat gcaagcggag cgatgtcccg tttaccggtc ggtatcaatg caacgcgtca
    24721 gattgcagcg gcacaacagg aaatggcccg ggaggcgtcg aacagagcag aaagtgccct
    24781 gcatggtttc agcagcagta ttgccagtgc ctggaacacg ctcagtcagt ttggttctaa
    24841 ccgggggagc agtgattctg tcaccggtgg cgctgacagc accatgagtg cacaggactc
    24901 catgatggcc agccgtatgc gcagtgcagt ggaaagctat gcgaaggcgc ataatatcag
    24961 taatgagcag gcgactcagg aactggcatc aacaagtacc aggctatctg gtggaatgta
    25021 tgctgatgct catgctgaat ggggggttaa acctaaaatt cttggtgttg gtggtggggt
    25081 aggcatcaga ggtggattta aggggagcct ggatgcaacg gatgatgact cacattcagc
    25141 aagtagtggc agcagggcta cccatgatgc tcgccatgat atcgatgcta aagccagtaa
    25201 ggactttaaa gaggccagcg attactttac cagtcgcaaa gtcagtgaat ccggcagcca
    25261 tactgacaat aatgccgatt cccgcgtgga ccagttgtct gcagccctga actcagcaaa
    25321 acagagttac gaccagtaca cgacgaatat gacccgcagc catgaatatg cggaaatggc
    25381 ctcccgtact gaaagcatga gcgggcagat gagtgaagac ctgtcgcaac agtttgcaca
    25441 gtatgtgatg aaacgcgcac cgcaggatgc agaagctatt ctgacaaata ccagttcgcc
    25501 ggaggttgca gaacgccgtc gggctatggc gtggtctttt gtgcaggaac aggtacagcc
    25561 tggtgttgat aatgcctggc gtgaatcccg tggggatatt ggtaaaggaa tggagagcgt
    25621 accttcgggt ggtggcagcc aggatattat tgctgaccat caggggcatc aggccattat
    25681 tgatcaaaga acgcaggaca gtaatatccg taatgatgta aaacatcagg ttgataatat
    25741 ggtcacagaa tataaaggga atattggata tactcagaat agtattcatg gcgaagaaaa
    25801 tattgtagac agacaatatt cagagttaaa aaataatcac aaacaggaag aaattcagca
    25861 aaacaacaga tataatgaag aaaataaacg acagaaatta atgccaacac cgtctgaaga
    25921 tgcgttaaaa cagatggtgg atgataagaa agaacgattg aaggggccat tatgatcact
    25981 cactatgata taaaaatgga gatgcaaaaa ctaaaggagg tactctctgt agagggtgtt
    26041 aatatcccat cattattaca agtcataaag ccgggaactt atgtgttttt atgggtttta
    26101 ttatggccaa cttttctgcg tttagtatcg gttaaaagtg atgtcaggga tgttggcttt
    26161 gatatatgtg ctagtggaat gatggggttc ctgctttttg tggctattac aaatggaatg
    26221 atgttatacc ttgcgattcc tgatagtttc agaaaggatt caaaaataat aaattttatg
    26281 tattctaaga gtaagacata tattttgttg tttttgattg ttttttctat ggtttcattt
    26341 atgcattcta tcttatatgt ttttgcacta atgattacat tcatattatt ttttttggtg
    26401 tataccatag atataaatcg ttataatctc tcagctatag cctcagtaat aggactgttc
    26461 aaaaaagagt ctgttagtta aaaggatatt ttggatgaaa acaaaaaaat tgatgatggt
    26521 tgcactggtc agttccactc tggccctttc agggtgtggt gcgatgagca cagcaatcaa
    26581 gaagcgtaac cttgaggtga agactcagat gagtgagacc atctggctta aacccgacag
    26641 cgaacgcacg gtatttctgc agatcaaaaa cacgtctgat aaagacatga gtgggctgca
    26701 gggcaaaatt gctgatgctg tgaaagcaaa aggatatcag gtggtgactt ctccggataa
    26761 agcctactac tggattcagg cgaatgtgct gaaggctgat aagatggatc tgcgggagtc
    26821 tcagggatgg ctgaaccgtg gttatgaagg cgcagctgtt ggtgcagcgt taggtgccgg
    26881 tattaccggc tataactcaa attctgccgg tgccacactc ggtgtaggcc ttgctgctgg
    26941 tctggtgggt atggctgcag atgcgatggt ggaagatgtg aactatacca tgatcacgga
    27001 tgtacagatt gcagagcgta ctaaggcaac ggtgacaacg gataatgttg ccgccctgcg
    27061 tcagggcaca tccggtgcga aaattcagac cagtactgaa acgggtaacc agcataaata
    27121 ccagacccgt gtggtctcaa atgcgaacaa ggttaacctg aaatttgaag aggcgaagcc
    27181 tgttctcgaa gaccagctgg ccaaatcaat cgcaaatatt ctctgaacct cgtcaggagg
    27241 ctgatctgac cggcctcctt gctgacgcct cgcgttgctc gttgtccaac cccgggatgt
    27301 aacaaaataa attttgtcac ctcccggcgt ttccgaaata aatatgaaaa cactgagcgg
    27361 ttgtgcaaat tctttgtgcg accgtgaagt gttgccggcg aagccgggat cttccggaga
    27421 aagtcattat gacatcagac aatatcgctg aatatgttgg tgagaataat caatttactg
    27481 agaagcctgt aactgaatac cccccggtga tacaggataa tacagtgacc actaaagact
    27541 atctgtacag caataatatt gatactgacc ttttagtggt tatcggaggg cttattgtta
    27601 tattgtttct catgatgctt atcatgtact ttattaattc tctcaggcat aaggaattaa
    27661 ctgaaatact gacaaaaata tcttttcctg aaaaatccgg gagcaattcg cttaaaccgg
    27721 aagcggtcag ctgtaatttg ttgtccctgt gtgtgctggc accggtattc tgtacaccca
    27781 gaaagctggc agaagtctcc agggagatca tcgtggcgag tgcgcaggaa attgcatgga
    27841 cgccggggag cagtcgtatt gatggtcatc agggcgaaaa tattacctgg tctctgtcat
    27901 cgcagtctcc tgccgataag tatgatctca tcaataaaac gtcccgtgtt tctcctgaca
    27961 gtggccgttt ttatgaaccg gacacagttc tggatcaccg tgaacgggta cgccttttta
    28021 ttcagatcca gtttccgcac gggagtgact ggtctgaagt attgcgcctg actgacattg
    28081 ttacagagaa ggtctcttcc ggcagggctg caggcgggta tcaccaccgg catacagggt
    28141 acacctggtc tgtttctgtg aattaacccc acatcatcct tttctgccct gttccagatt
    28201 ttcccggagt ttcatcatga gttttaacgc aaaggatatg acccagggcg gtcagattgc
    28261 gtccatgcgt atccgcatgt tcagccagat cgccaatatc atgctttact gcctgtttat
    28321 ttttttctgg atactcgttg gtctgatttt atgggtaaaa ataagctggc agacgtttgt
    28381 gaacggctgt atttactggt ggtgtaccac gctggaaggc atgcgggatt taatcaagtc
    28441 ccagccagta tatgagattc agtattacgg caaaaccttc cggatgaacg ctgcccaggt
    28501 actgcatgat aaatatatga tctggtgcgg tgagcagcta tggtccgcat tcgttctggc
    28561 cacagttgtg gcactggtta tttgcctgat taccttcttt gttgtctcct ggattctggg
    28621 gcgtcagggt aaacaacaga gcgaaaatga agtcacaggt ggtcgtcagc tgacagacaa
    28681 tccgaaagac gttgcccgga tgctgaaaaa agacggcaag gactccgata tccggattgg
    28741 cgacctgccg attatccggg attccgaaat ccagaacttc tgtctgcacg gcacggtcgg
    28801 ggccggtaag tcggaggtta tccgtcgtct ggccaactac gcccgtcagc gcggtgatat
    28861 ggtggtgatt tatgaccgtt caggggaatt tgttaaaagt tactatgatc cctccatcga
    28921 taaaatcctg aatccgctgg atgcccgctg tgccgcatgg gatttgtgga aggagtgcct
    28981 gacacagccg gattttgata ataccgcaaa tactctgatc cctatgggta caaaagagga
    29041 cccgttctgg cagggttcag gacgtaccat ttttgcggaa gcggcgtacc tgatgcgtaa
    29101 tgaccccaac cgcagctaca gcaaactggt ggacacactg ctttccatca aaattgaaaa
    29161 actgcgtacc ttcctgcgta attcaccggc ggccaacctg gtggaagaga aaattgagaa
    29221 aacggcaatt tccatccgtg ctgtgctgac caactacgtg aaagctatcc gttacctgca
    29281 ggggattgag cataacggtg agcccttcac catccgtgac tggatgcggg gtgtccggga
    29341 agatcagaaa aacggctggc tgtttatttc gtcgaatgcc gacacccatg cctccctgaa
    29401 accggtgatc tccatgtggc tgtccattgc cattcgtggt ctgctggcaa tgggagaaaa
    29461 tcgtaaccgt cgtgtgtggt ttttctgtga cgagttaccc acgttacaca aactgccgga
    29521 cctggtggag atcctgccgg aagcccgtaa gttcggtggc tgttatgtgt ttggtatcca
    29581 gtcctatgcc cagctggaag atatctacgg tgagaaagcg gctgccacgc tgtttgacgt
    29641 catgaacacc cgtgcctttt tccgttctcc cagccataag attgcagagt tcgctgccgg
    29701 tgagattggt gagaaagagc acctgaaagc cagcgagcag tattcctacg gtgctgatcc
    29761 ggtacgtgac ggggtatcga ccggtaagga tatggagcgc cagacgctgg tcagttattc
    29821 cgacattcag tctctgccgg atctgacctg ttatgtcacc ctgcccggac cgtatccggc
    29881 agtaaaactc tcactgaaat atcaggcacg tccgaaggtt gcccctgagt ttattccgcg
    29941 tgacatcaac ccggaaatgg agaaccgtct gagtgccgta cttgccgcaa gggaagcaga
    30001 aggtcgtcag atggccagcc tcttcgaacc ggatgtgccg gaagttgttt ccggagaaga
    30061 cgtgactcag gctgaacagc cgcaacagcc gcaacagccg caacagccgc aacagccgca
    30121 acagccgcaa cagccggtgt ctcctgccat caacgataag aagtcagatt caggtgtgaa
    30181 tattccggca ggggggatcg agcaggagct gaaaatgaaa ccggaagaag agatggaaca
    30241 gcaactgcca cccgggatca gtgaatccgg tgaagtggtg gatatggccg cttatgaggc
    30301 atggcaacag gaaaatcatc cggacatcca gcagcagatg cagcgtcgtg aagaggtgaa
    30361 cattaatgtg caccgggagc gtggggagga tgttgagccg ggagatgatt tctgatgatg
    30421 agtatcgcgc aggtcagatc ggccggaagt gccggtaact attataccga caaggataat
    30481 tactatgtac tgggcagcat gggagaacgc tgggccggca agggggctga acagcttggg
    30541 ctgcagggca gcgtcgataa ggatgttttt acccgtcttc tggagggaag gctgccggac
    30601 ggagcggatc taagccgcat gcaggatggc agtaataagc atcgtcccgg ctacgacctg
    30661 accttctccg cccccaaaag tgtctccatg atggccatgt taggtggcga taagcgcctg
    30721 attgatgcac ataaccaggc cgtggacttt gctgttcgtc aggtggaggc gctggcctcc
    30781 acacgggtga tgacggacgg acagtcagaa acggtgctga ccggtaattt ggtgatggca
    30841 ctgtttaacc acgacaccag tcgcgatcag gaaccacagt tacacacgca tgcggtggtg
    30901 gctaatgtca cgcagcataa cggtgagtgg aagacactga gcagtgacaa agtggggaaa
    30961 acggggttca ttgagaatgt gtacgctaat cagattgcct ttggcaggct ctaccgggaa
    31021 aaactgaaag agcaggttga ggcgctgggc tatgaaaccg aagtggtggg taagcacggt
    31081 atgtgggaga tgccgggcgt accggtggag gccttttccg gacgctcaca ggctatccgg
    31141 gaggccgtcg gggaagacgc ctcgctgaaa tctcgggatg tggcggcact ggatacgcgt
    31201 aaatccaaac agcacgtcga tccggaaatc agaatggctg aatggatgca gacgctgaag
    31261 gaaaccgggt tcgacatccg ggcatatcgt gacgcggcgg atcagcgtac ggagatccgc
    31321 acgcaggcgc ccgggccagc ttcacaggac gggccggatg tgcagcaggc ggtgacacag
    31381 gcgattgccg gattaagtga acgaaaagtg cagttcacgt acacggacgt gctggccagg
    31441 acggtcggca tactgccgcc ggaaaatggt gtgattgaac gggcacgcgc cggtatcgat
    31501 gaggccatca gccgtgagca gcttatcccc ctcgatcgtg agaaggggct gttcacgtcc
    31561 gggattcatg tgctcgatga gctgtcagtc cgggcactca gtcgtgacat catgaaacag
    31621 aaccgggtga ccgtacatcc ggagaaaagt gtcccccgga cggccggtta cagcgatgcc
    31681 gtcagtgttc tggcacagga tcgcccgtcg ctggccattg tgtccgggca gggcggtgca
    31741 gccgggcagc gtgagcgggt ggctgaactg gtcatgatgg cccgggaaca ggggcgggag
    31801 gtgcagatta tcgctgctga ccgtcgctcg cagatgaacc tgaagcagga tgaacggctg
    31861 tccggtgaac tgataaccgg acgtcgtcag ctgctggaag gcatggcctt cacgccgggc
    31921 agtaccgtta tcgtcgacca gggcgaaaaa ctctccctga aagagacgtt aaccctgctg
    31981 gatggtgccg cacgtcataa cgtacaggtc ctgataaccg acagcgggca gcgaaccggt
    32041 acaggcagtg cgctgatggc catgaaggat gccggggtga acacatatcg ctggcagggc
    32101 ggagaacagc gaccggccac catcatcagt gaaccggacc gtaatgtccg ctatgcccgg
    32161 ctggccggag attttgcggc cagcgtgaaa gccggagaag agagcgtggc acaggtcagc
    32221 ggggtacggg aacaggccat actgacacag gccattcgca gtgagctgaa aacgcagggc
    32281 gtgctcggac accctgaggt gaccatgact gcactgtcac cggtctggct ggacagccgg
    32341 agccgttatc tgcgggatat gtaccgaccg gggatggtga tggagcagtg gaacccggag
    32401 acacgcagtc atgaccgcta tgtgatagac cgggtgacag cgcagagtca cagcctgacc
    32461 ctgcgggatg cgcagggcga aacgcaggtg gtgcgtattt cctccctgga cagcagctgg
    32521 tcgctgttcc ggccggaaaa aatgccggtg gcagacggcg agcgactgag ggtgacaggg
    32581 aaaattcccg gactccgcgt ctccggcggt gaccgcctgc aggtggcatc cgtcagtgaa
    32641 gatgcgatga cggttgttgt gccggggcgg gctgaaccgg cctccctgcc tgtgagcgat
    32701 tcaccgttca cggcactgaa gctggagaac ggctgggtgg aaacgcccgg gcattccgtc
    32761 agtgacagtg cgacggtgtt tgcctccgtc acacagatgg caatggacaa cgccaccctg
    32821 aacggtctgg cccgcagcgg tcgtgatgtc cggctgtatt cctcactgga tgaaacccgt
    32881 actgcggaaa aacttgcccg ccatccctcc tttacggtgg tttctgagca gataaaggcg
    32941 cgggccggtg agacatcgct ggaaaccgct atcagtctgc agaaagccgg gctgcacacg
    33001 ccggcacagc aggccattca tctggccctt cctgtgctgg aaagtaaaaa cctggccttc
    33061 agcatggtgg acctgctgac agaggcgaag tcgtttgctg cagaaggaac cggttttact
    33121 gaactgggag gggaaatcaa tgcgcagata aaacgcggtg acttactgta tgtggatgtg
    33181 gcaaaaggct atggcacagg cctgctggtt tcccgtgcgt cgtatgaggc agaaaaaagt
    33241 attcttcgcc atattctcga aggtaaggag gcggtcacgc cgctgatgga gagagtaccc
    33301 ggcgaactca tggagacgtt aacgtcggga cagcgtgccg ccacccgtat gatactggaa
    33361 acgtccgacc gcttcacggt ggtacagggt tatgccggtg tgggtaagac cacacagttc
    33421 cgggcggtga tgtcagccgt gaacatgctg ccggcgagtg agcgtccccg ggttgtgggg
    33481 ctggggccca cgcaccgcgc ggtcggggag atgcgcagcg ccggcgtgga tgcacagacg
    33541 ctggcgtcct ttctgcatga cacgcagctg cagcagcgca gcggagaaac gccggatttc
    33601 agcaacacgc tgttcctgct cgatgagagc tcaatggtgg gcaataccga catggcacgg
    33661 gcatacgccc tgattgcggc cggtggtggt cgtgctgtgg ccagcggtga cacggaccag
    33721 ctgcaggcca tcgcgcccgg tcagcctttc cgtctccagc agacgcgcag tgctgccgat
    33781 gtggccatca tgaaggagat tgtgcgtcag acgccggaac tgcgggaggc ggtatacagc
    33841 ctgattaacc gggatgtgga aagggcactg tccgggcttg agagtgtgaa accgtctcag
    33901 gtgccacgtc tggagggcgc atgggcaccg gagcactccg tgacggagtt cagtcacagc
    33961 caggaagcga aactggcaga agcgcagcag aaggcgatgc tgaaaggcga ggctttcccg
    34021 gatatcccca tgacactgta tgaggccatt gtccgcgact ataccggcag gacaccggaa
    34081 gcacgggagc agacgctgat tgtcacgcac ctgaatgagg accggcgcgt actgaacagc
    34141 atgattcatg atgcacggga aaaggccggt gagctgggca aagagcaggt catggtgcct
    34201 gtcctgaaca cagcgaatat acgtgacgga gaactgcgtc gtctctccac ctgggagaaa
    34261 aacccggatg cccttgccct ggtggataat gtgtatcacc ggattgccgg tatcagcaag
    34321 gatgacgggc tgataaccct gcaggatgcg gaaggtaaca cgcggctgat ttcgccccgg
    34381 gaggcggtgg ctgaaggtgt cacactgtac acaccagaca aaatccgggt gggaaccggc
    34441 gaccggatgc gcttcacgaa gagtgaccgg gagcgcggtt atgtggccaa cagcgtctgg
    34501 acggtgacag cggtttccgg tgacagtgtc acgctgtcag acggacagca gacccgggtg
    34561 attcgccccg gccaggagcg ggcagagcaa catattgacc tggcttatgc catcaccgcc
    34621 cacggtgcgc agggggcgag tgaaaccttt gccatcgcgc ttgaaggcac ggaaggtaac
    34681 cggaaactga tggccggctt tgagtcagcc tacgtggccc tgtcgcgtat gaagcagcat
    34741 gtgcaggtgt acaccgataa ccgtcagggc tggacggatg ccattaacaa tgccgtacag
    34801 aaaggaactg cccacgatgt gctggagccg aaaccggacc gggaggtcat gaatgcgcag
    34861 cggctgttca gtacggcacg ggaactgcgg gacgtggcgg caggccgagc cgttcttcgt
    34921 caggcggggc tgtccggggg agacagtcct gcacggttta ttgctccggg acgtaaatat
    34981 ccacagccgt atgtggcact gccggctttt gaccgtaacg gcaggtctgc cggtatctgg
    35041 ctgaacccgc tgaccacgga tgacggaaac gggctgcggg gattcagtgg tgaaggccgg
    35101 gtgaagggca gtgaagatgc acagtttgtg gccctgcagg gcagccgtaa cggagaaagc
    35161 ctgctggctg ataatatgca ggatggtgtc cggattgccc gtgataatcc tgacagtggt
    35221 gtggtggtaa gaatcgccgg tgaaggccgt ccgtggaatc ccggtgccat aaccggtggt
    35281 cgcgtgtggg gggatatccc ggacaacagc gtccagccgg gagccggaaa tggcgaaccg
    35341 gtcacggcag aggtgctggc acagcggcag gctgaagagg ccatccgccg tgaaacggaa
    35401 cgccgcgcag atgaaattgt cagaaaaatg gcagagaaca aacctgacct gccggatggt
    35461 aaaacagagc aggctgtcag ggaaattgcc gggcaggagc gtgaccgggc tgacataact
    35521 gaacgggaag ccgcgctgcc ggagagtgtg ctgcgtgaat cacaacggga gcaggaagcg
    35581 gtcagggagg ttgcccggga aaatctgctg caggagcgtc tgcagcagat ggagcgggat
    35641 atggttcgtg acctgcagaa agagaaaaca ctgggcggag actgatacag gaagacaaac
    35701 actgatgaca acggataaca cgaacacgac acgtaacgat tcactggctg cacggaccga
    35761 tacctggttg cagtcatttc tggtctggtc acccggacag cgggatatca tcaaaacggt
    35821 ggcactggtg ctgatggtac tggaccacat caatctgata ttccagctga agcaggaatg
    35881 gatgtttctg gccgggcgag gagcctttcc gctgtttgcc ctggtgtggg ggctgaatct
    35941 gtcccgtcat gcgaatatcc ggcaaccggc cattaaccgg ctgtggggat gggggattat
    36001 tgcccagttc gcatattatc tggccggctt tccctggtat gaggggaata tcctgtttgc
    36061 ctttgcagtg gcggcacagg tgctgacgtg gtgtgaaacg cgcagtgggt ggcgtacagc
    36121 agcggccatt cttctgatgg ccctgtgggg gcccttgtcc ggcaccagtt acggcattgc
    36181 cgggctgctg atgctggcag tcagccaccg gctgtaccgg gcggaagaca gagcggaacg
    36241 tctggcgctg gtcgcctgcc tgctggctgt tattccggcg cttaaccttg ccaccagtga
    36301 tgcagcggcg gtggctggtc ttgtgatgac ggtgctgacc gttggtctgg tgtcgtgtgc
    36361 aggaaaatca ttaccccgtt tctggcccgg ggattttttc ccggtattct acgcctgtca
    36421 tctggctgtg ctgggcgttc tggcgctgtg acgggtgtgg tatctttgac cgtaacagga
    36481 tgattcgtca gaggcagaac acagcatgac agagcagaag cgaccggtac tgacactgaa
    36541 gcggaaaacg gaaggggaga cgcctgtccg cagccggaaa accatcatca atgtcaccac
    36601 gccaccaaag tggaaggtga aaaagcagaa gctggccgag aaagcagccc gggaagcaga
    36661 gctggcggca aaaaaagcgc aggccagaca ggcactgtcc atttatctga acctgcccac
    36721 gctggatgag gccgtgaata ccctgaaacc ctggtggccg ggatt
//
LOCUS       DBKEKI010000026        36314 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.026, whole genome shotgun sequence.
ACCESSION   DBKEKI010000026 DBKEKI010000000
VERSION     DBKEKI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.026"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(16..195)
                     /locus_tag="ACUQPX_001289"
     CDS             complement(16..195)
                     /locus_tag="ACUQPX_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HHV9056049.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(279..1163)
                     /gene="yhdJ"
                     /locus_tag="ACUQPX_001290"
     CDS             complement(279..1163)
                     /gene="yhdJ"
                     /locus_tag="ACUQPX_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HHV9056050.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1249..1545)
                     /gene="fis"
                     /locus_tag="ACUQPX_001291"
     CDS             complement(1249..1545)
                     /gene="fis"
                     /locus_tag="ACUQPX_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HHV9056051.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1571..2536)
                     /gene="dusB"
                     /locus_tag="ACUQPX_001292"
     CDS             complement(1571..2536)
                     /gene="dusB"
                     /locus_tag="ACUQPX_001292"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HHV9056052.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2865..3746)
                     /gene="prmA"
                     /locus_tag="ACUQPX_001293"
     CDS             complement(2865..3746)
                     /gene="prmA"
                     /locus_tag="ACUQPX_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HHV9056053.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLYDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRNNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3758..5209)
                     /gene="panF"
                     /locus_tag="ACUQPX_001294"
     CDS             complement(3758..5209)
                     /gene="panF"
                     /locus_tag="ACUQPX_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HHV9056054.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TVVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5199..5441)
                     /locus_tag="ACUQPX_001295"
     CDS             complement(5199..5441)
                     /locus_tag="ACUQPX_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HHV9056055.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5550..6899)
                     /gene="accC"
                     /locus_tag="ACUQPX_001296"
     CDS             complement(5550..6899)
                     /gene="accC"
                     /locus_tag="ACUQPX_001296"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HHV9056056.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6910..7380)
                     /gene="accB"
                     /locus_tag="ACUQPX_001297"
     CDS             complement(6910..7380)
                     /gene="accB"
                     /locus_tag="ACUQPX_001297"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HHV9056057.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8358..9332)
                     /locus_tag="ACUQPX_001298"
     CDS             complement(8358..9332)
                     /locus_tag="ACUQPX_001298"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HHV9056058.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9484..11424
                     /gene="csrD"
                     /locus_tag="ACUQPX_001299"
     CDS             9484..11424
                     /gene="csrD"
                     /locus_tag="ACUQPX_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HHV9056059.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11729..12772
                     /gene="mreB"
                     /locus_tag="ACUQPX_001300"
     CDS             11729..12772
                     /gene="mreB"
                     /locus_tag="ACUQPX_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HHV9056060.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12838..13941
                     /gene="mreC"
                     /locus_tag="ACUQPX_001301"
     CDS             12838..13941
                     /gene="mreC"
                     /locus_tag="ACUQPX_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HHV9056061.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13941..14429
                     /gene="mreD"
                     /locus_tag="ACUQPX_001302"
     CDS             13941..14429
                     /gene="mreD"
                     /locus_tag="ACUQPX_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HHV9056062.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14438..15031
                     /gene="yhdE"
                     /locus_tag="ACUQPX_001303"
     CDS             14438..15031
                     /gene="yhdE"
                     /locus_tag="ACUQPX_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HHV9056063.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15021..16490
                     /gene="rng"
                     /locus_tag="ACUQPX_001304"
     CDS             15021..16490
                     /gene="rng"
                     /locus_tag="ACUQPX_001304"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HHV9056064.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16558..20358
                     /gene="yhdP"
                     /locus_tag="ACUQPX_001305"
     CDS             16558..20358
                     /gene="yhdP"
                     /locus_tag="ACUQPX_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HHV9056065.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20514..21959
                     /gene="tldD"
                     /locus_tag="ACUQPX_001306"
     CDS             20514..21959
                     /gene="tldD"
                     /locus_tag="ACUQPX_001306"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HHV9056066.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22087..23016)
                     /gene="aaeR"
                     /locus_tag="ACUQPX_001307"
     CDS             complement(22087..23016)
                     /gene="aaeR"
                     /locus_tag="ACUQPX_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HHV9056067.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23199..23402
                     /gene="aaeX"
                     /locus_tag="ACUQPX_001308"
     CDS             23199..23402
                     /gene="aaeX"
                     /locus_tag="ACUQPX_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HHV9056068.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23410..24342
                     /gene="aaeA"
                     /locus_tag="ACUQPX_001309"
     CDS             23410..24342
                     /gene="aaeA"
                     /locus_tag="ACUQPX_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HHV9056069.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24348..26315
                     /gene="aaeB"
                     /locus_tag="ACUQPX_001310"
     CDS             24348..26315
                     /gene="aaeB"
                     /locus_tag="ACUQPX_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HHV9056070.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26407..26679
                     /locus_tag="ACUQPX_001311"
     CDS             26407..26679
                     /locus_tag="ACUQPX_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HHV9056071.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26735..26998)
                     /gene="yhcN"
                     /locus_tag="ACUQPX_001312"
     CDS             complement(26735..26998)
                     /gene="yhcN"
                     /locus_tag="ACUQPX_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HHV9056072.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27364..27834)
                     /gene="argR"
                     /locus_tag="ACUQPX_001313"
     CDS             complement(27364..27834)
                     /gene="argR"
                     /locus_tag="ACUQPX_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HHV9056073.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28269..29207
                     /gene="mdh"
                     /locus_tag="ACUQPX_001314"
     CDS             28269..29207
                     /gene="mdh"
                     /locus_tag="ACUQPX_001314"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HHV9056074.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVILISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29269..30336)
                     /gene="degS"
                     /locus_tag="ACUQPX_001315"
     CDS             complement(29269..30336)
                     /gene="degS"
                     /locus_tag="ACUQPX_001315"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HHV9056075.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30426..31793)
                     /gene="degQ"
                     /locus_tag="ACUQPX_001316"
     CDS             complement(30426..31793)
                     /gene="degQ"
                     /locus_tag="ACUQPX_001316"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HHV9056076.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASILGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31947..32345)
                     /gene="zapG"
                     /locus_tag="ACUQPX_001317"
     CDS             complement(31947..32345)
                     /gene="zapG"
                     /locus_tag="ACUQPX_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HHV9056077.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32539..33666
                     /gene="zapE"
                     /locus_tag="ACUQPX_001318"
     CDS             32539..33666
                     /gene="zapE"
                     /locus_tag="ACUQPX_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HHV9056078.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33885..34313
                     /gene="rplM"
                     /locus_tag="ACUQPX_001319"
     CDS             33885..34313
                     /gene="rplM"
                     /locus_tag="ACUQPX_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HHV9056079.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34329..34721
                     /gene="rpsI"
                     /locus_tag="ACUQPX_001320"
     CDS             34329..34721
                     /gene="rpsI"
                     /locus_tag="ACUQPX_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HHV9056080.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35124..35762
                     /gene="sspA"
                     /locus_tag="ACUQPX_001321"
     CDS             35124..35762
                     /gene="sspA"
                     /locus_tag="ACUQPX_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HHV9056081.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35768..36265
                     /gene="sspB"
                     /locus_tag="ACUQPX_001322"
     CDS             35768..36265
                     /gene="sspB"
                     /locus_tag="ACUQPX_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HHV9056082.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
BASE COUNT         8736 a         9050 c         9590 g         8938 t
ORIGIN      
        1 aagtgtcatg taaattcaca ttctgtcctg aaaattcagt tcgtcacagt taaccagttt
       61 tagtggatta actgagttca tattacggca tttgtctgtt tctgttgtca gcagttcaat
      121 ccactgcatc agtaaagtat caaacagaaa aacgaagaca gcgaaaacga cgagccacca
      181 atacttgcga atcattacct gagcctgtgc atgtttaacc aagtctggca ggaaatatac
      241 acgaaatgaa taatctgaaa accaggctta cacttccttt actttgcaat gagatcgggg
      301 tcaacttcgc ttaaccggct tcgttttgac aggttgccgg tctttctttt tttcactttc
      361 gccagttctt ccgcggagta atgcgacgcg acatccagcc gtcgaagccc cattttgatg
      421 tactcgctgt tgatctcaat accaatgaat tttcgtccgc tggcgatggc tacggcaccg
      481 gtagtaaagc taccggcaaa cgggtcgaga acgatatcgc ctgggttgga agaggcgaga
      541 ataatgcgtt tcagtaaggc tttcggtttt tgcgtcgggt ggttctcata ttcatccatc
      601 aaataacgca cgcgcggaaa atcccagacg ttacccggta ctttttgatg attgtatggc
      661 tgtggaggat ttttgcgata atcgatcaac gcgcgctgcg atccggtttt ggcttctacc
      721 agaatagcat caccgttgaa tgtgtagttc tttgcgtctt tcaccatcat caggatgggt
      781 tcgtacatgg agccgtagtg ttttttcgct tgtactccag aactgtcata tgaccagacg
      841 atgcgacttt tgatggtaaa aagcttgcgg cactggagat cgataaaggg catgttttcc
      901 gtactgttca taatgtacat gctgccctgc tttttcagaa cgcggtggca ctctgcaatc
      961 acttcaaaca gccagtcgat aaacagatct tctttccagg cttcgatcag accatcaaaa
     1021 tttttaccga tgttatacgg tgggtcggca aagagcagat cgacgctttc agtaggtagc
     1081 tttttaagtt cggcaagggc atcaccgtga ataatggtct tagcttcatt accaaaccgg
     1141 gtcggttcac atcctgttct catggtcact ccctttgtga cacctataaa aaaggcgctt
     1201 ccccatgccg agtagcgcct ttttaatcaa gcatttagct aacctgaatt agttcatgcc
     1261 gtattttttc aattttttac gcagcgtacc acggttgatg cccatcatca gcgcagcacg
     1321 ggtctggtta ccacgggtgt attgcatcac catgtccaac aggggctgtt ctacttcagc
     1381 cagtaccagc tcatagaggt cattcacatc ctgaccattc agttgagcaa aatagttctt
     1441 cagtgcctgt ttaaccgagt cacgcagggg tttttgggtt acctgatcct gagagttaac
     1501 ggtagaaacg gtcagtacgt cagaatttac gcgttgttcg aacatagttc tgtcagctct
     1561 ttatttctgt ttacgcaaaa ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg
     1621 catcctcaat ggcgttgaat gtgcgccgaa actggtcatt tggagcgtgt tcctggagat
     1681 accaggaaac gtgtttacgt gcaattcggt acccttttgc cggaccataa aagtcatgca
     1741 gttcccgaac gtgcgcgcaa agcaagcgct taacctctgc caaaggcagt gggggcagca
     1801 actccccagt gtccagataa tgctggattt cccgaaagat ccagggtctt ccctgagctg
     1861 cgcggcctat catcagggca tccgcccctg tatagtcgag cacagctctg gctttaagcg
     1921 ggtcagtaat gtcgccattc gcgataaccg gaatggaaac tttctgctta actgcccgaa
     1981 tactgtcgta ctcagcttct ccattgaaca aacaggcgcg tgtacggcca tgaatggtca
     2041 gagcctgaat gccacagtct tcagccagtt gggcaatctc ttcgcagtta cggtgttccg
     2101 gtgcccagcc ggtgcgaatc ttcagggtaa caggaacgtc cactgcattg acgacctcgg
     2161 taaggatcga tttaacgaca tccgggtact gcaagagggc tgaacctgcg agcttgcgat
     2221 tcactttttt agccgggcaa cccatattga tatcaataat ctgggcaccg ctttccacgt
     2281 taatacgtgc tgcatctgcc atttctttcg gagcgctacc agcaatttgc acggtgcgaa
     2341 taccgggttc atcaatgtgc accatccgta aacgagattt gtcgctttcc caaacctgtg
     2401 ggttagaaga catcatctcg gatactgtca atccggctcc catctcgtag cacaacgtcc
     2461 gaaaaggtct gtctgtaatg ccagccatgg gcgctgcgat caggcgattt ctgagctgat
     2521 attgtccgat gcgcatgagt taagaaatga ccatactgtg actgcaaggc ggcgtatatt
     2581 acgcattttt tgcacgagat gaaaggccaa actttgacca atcctctgag atggatcaaa
     2641 gaattgcatt taaaatgagc gtggtgcgat aattactcat aaaaatcatc atattagaaa
     2701 atagtgacta aaatttacac tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa
     2761 tgaaaatttt ggcacgcaaa tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag
     2821 cttgctcgca cttcgccccg cgtcacccta cggcgatgcg aaggttaatt cttacgaccg
     2881 gtaatacggc accactcttc tttttccacg accgggtcca gtgcgaagct atcggcataa
     2941 gcttcacaaa cgctctctgc ctggcttgcc agaataccgg aaaggcccag caaaccgcct
     3001 gaaaccggca ggacgctgat taacggtgcc agttcacgta atgggcctgc aaggatgtta
     3061 gcgaccacca cgtcggcttt catttcttct ggctgatctt tcggtaagta gagttccaga
     3121 cggtcagaaa cgccattacg ttcggcgtta ttgcggctgg cctgaatcgc ctgcggatcg
     3181 atatcaatac caatggcttt tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg
     3241 gaaccacagc caaagtcgat gactgtttta ccggttaaat cgaggctgtc gagccattgc
     3301 aggcacagag aggtggttgg atgggtaccc gtaccaaacg ccagccctgg atctaacatc
     3361 acgttgacgg cgttttcgtc cggcacatca cgccagctag ggcagatcca cagtcgttca
     3421 ccaaagcgca tcgggtggaa attatccatc cattcgcgct cccagtcttt atcttctagt
     3481 tgttcgattt tatgcgcgaa gcctgcgccg agcagcggat ggttttccag aatcgccacc
     3541 acgtcgttca tatcggtttc agcgtcgtac agaccaatca catcggtgtc gccccacagg
     3601 cgcgtttcgc ccggcagcgg ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata
     3661 gaaacggcac ccgcttccat cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg
     3721 ttcagtttca gttggatcca aggcatggca aaactcttta tttatcagta gtcaaaacgg
     3781 tagcttgtgg gacggatgta ccgaaacggt ttccgaccag gaaagccagc aaacttagta
     3841 gtaacgaggg cacgataggg tggaagccca ggtactgaat attcagcgtc gcgagtacgg
     3901 catacagcac gccgccaacg atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc
     3961 agtaaagacc cagcaccagc ggccacagga aaacggcttc cagcccaccg aaggccagca
     4021 aattcagcca gatgatcatt tctggcggct tccaggcggc aagcagcagc aacgcgccga
     4081 gaactaacgt aattaccgcc gacatccgct tcagacgcgt ctcgttttgc atttgatccg
     4141 gacggatatt cagatagaga tctttaatga tcgtagcgga actttgcagc agttgggcgt
     4201 taattgtcga catgatcgca gccataggtg cagccagaaa gatcccggca gcaaaaggtg
     4261 gcagcacttt taccattaac gtcgggatca ccagatccgg tacggtgaga tcggggatca
     4321 ctgcacgacc taacgctccg gccaggtgca taccgaacat caggattgcg actacaaccg
     4381 taccgatgat gatcccccgg tgtactgctt tgctgtcttt ataagagata cagcgcaccg
     4441 cagtatgcgg caggccaatc acgccaaagc acaccagtac ccagaacgac gtcataaagg
     4501 caggcgacag aatatcgtca gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca
     4561 aggtctgtac tgcgttgctt aagccgccag cggcatgtac cacgccaata agcagcacaa
     4621 cggtgccaat cagcatcaca agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc
     4681 caccaaaggc ggtatataac gcaatgctga taccaaaaat cagcagcccg gtttcataag
     4741 gaatacccgc cgcggtttcc agcaggcgcg caccgccgat aaactgcacg gtcatcgcac
     4801 caacgaacgc aaccagcaaa ctcaaactcg ccagccacac cagaagacga ctctggtagc
     4861 gggcaaacag catatcgttc agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact
     4921 tcttgccgag aataccgagt gaaagccaga cagcaggaag ctgaatcatc gccagcaata
     4981 cccagcccag cccgtattta taagcagctc ctggcccgcc gataaacgaa ctggcactga
     5041 tataggtcgc ggtgagcgtc atcgccagca caataccgcc catagagcgg ctgccgagga
     5101 aatactcatt aaggaaggtg ccggtgctcc gtttacgcat cgcataaacc gagataccga
     5161 acaccaccac cagataggcg accagcggta gaattacttc aagctgcatc gtcatcctcc
     5221 agtgggatat cgcgatagat aaatttcacc atcgcccagc acagtccaat aaacagtagc
     5281 ggcgtcagga tgcaggccat ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg
     5341 ccaggtaagt aagcgtctac taaccaaact gccagataca aaagggtcag ccccagcgcc
     5401 cagcgcgcct ctttatgggc ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg
     5461 aagaaagcga ggattgtacc ttatgggggt tgtcgatccc cagtaataaa aaaggccgga
     5521 aaatccggcc ttttgacgct tttgcagtct tatttttcct gaagaccgag ttttttctcc
     5581 agatagtgga tgttagtgcc accatgctgg aagttctcgt cattcatgat gcggatctgc
     5641 agatcaacgt tggttttgat accgtcgatg atcagctcct gcagcgcatt cttcatgcgg
     5701 gcaatcgcca cgtcacggtt ttcaccgtag caaatcagct taccgatcat tgagtcatag
     5761 tacggcggta cggtgtagcc cgcgtagata tgagactccc aacgtacgcc aaaaccgcca
     5821 ggtgcgtgga aacgggtgat tttgcctggg cttggcagga aggtgttcgg atcttcggcg
     5881 ttgatacgac attccaccgc atggccgcga acgtgaactt cttcttgctt gatcgacagc
     5941 ggttgaccgg cagcgatacg cagctgttct ttgatcaggt caacgccggt gatcatttct
     6001 gtaaccgggt gttctacctg aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt
     6061 tcgaacagga actcgaaagt acctgcaccg cgatagccga tatcaacaca cgctttagcg
     6121 caacgttcgc cgatgtagcg acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg
     6181 accactttct ggtggcggcg ctgcatggag cagtcacgtt ccgccagata gatagcgttg
     6241 ccctgaccgt cagccagtac ctgaatctcg acgtggcgag gattttccag gtatttctcc
     6301 atgtaaacca tatcgttgct gaaagcagct ttcgcttccg cacgggtcat ggagatggat
     6361 tgtgccagtt cagcgtcgcc gcgcactacg cgcataccgc gaccgccgcc gccgccggag
     6421 gctttgataa tcaccggata accaatgcgt ttagcaatgg cgcggttttt atccatatcg
     6481 tcgcccagcg ggccgtcaga accaggtacg caaggaacgc ccgctttttt catcgccgcg
     6541 attgcggata ctttgtcgcc catcaggcga atggtttctg ctttcgggcc gatgaagata
     6601 aagccggagc gttcaacctg ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc
     6661 ggatggattg ctactgcgcc ggtgatttca gcggcgctga tgattgccgg gatgttcaga
     6721 taacttttta ctgacggagc agggccaata cagaccgttt catctgccag taatacgtgt
     6781 tttagatcgc gatccgcgct ggagtgcaca gcgacagtct tgatgcccag ttctttacag
     6841 gcacgaagaa tacgcaatgc aatctcgccg cggttggcaa taacaatttt atccagcatg
     6901 ttcgcctcgt tactcgatga cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc
     6961 gaccagaatt gctttcacgg taccggattt gtccgcttcg atctggttca tcattttcat
     7021 ggcttcaacg atgcacaggg tatcgcccac gttgactttc tgacccactt cgatgaacgc
     7081 ttttgcgtcc gggcttgggg tgcggtagaa agtaccaacc atcggggaac gtacgatgtg
     7141 accactgatt tccgctgctg ctggcgcttc catggaagga acggtcgccg gagctgctgc
     7201 gttagattga gctggctgct gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa
     7261 acttgcggca ggagctgcac ggctaatgcg tactgactct tcgccttcag aaatttccag
     7321 ttcggagatg cctgattctt caaccagctc gatcagtttt ttaatcttac gaatatccat
     7381 gagtgggttc cgtactcttt gtttagtgtg attgtgacag gcgtttcacc gccgtctgta
     7441 aagcgtatgc cccgacagct gtatgcatag cgataaattc cagcaggccg ggacgccggt
     7501 ctattttgcc ccagagtgtc aatgttagac ttgacggaca ttgtgcagcg atctgctgct
     7561 atcctccggc aaaaaacaaa atataccttc gttgcgccaa tgtcaccttt tccgcagcgc
     7621 aaaaactgcg tcagggagga cgaggtcgca cattataacg atttcgtagc aattggcagc
     7681 taaatactgg tcttatcagg gaagataatc aacagctaac atgtaaataa ccttcaacac
     7741 cgtgtaattt gtaacaagcc gcacaattca cgaaattagc gccatcatcg acggaacttc
     7801 ttataacgta aggtgaaaag ctgaaaagcc agccctgcga agatgagggg ctgcaatgag
     7861 ataatcttca cagaccacaa ataatgtagg gatgccagga tcgcgacaag atagacgaca
     7921 ttacgcagcg gttgctgggg attacccaat tttcactgca tcgtctggat agatgtgaat
     7981 gctaaagcac gcaaaatcaa ccaactgaca attctcagca ttaaataggt tcgagtggtt
     8041 aatttgtagt caaataaaat cagattattc accccatggt accagaaatc agtaacaggt
     8101 taagcgtagt gtcgcccagg caaagcacca taaacctaac aggcgacgga ggcgtatcaa
     8161 taatggctgt ttagaggagt atcaatgcgc atcaatttgt ggtcagtcga aatcatgact
     8221 ttccagtttg gatgatgttg tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg
     8281 ctaaccgctt ttcccgttaa ccgattgaat aatgatgtca aaatgagttc cctctcttat
     8341 tattccttgc taaatggtta gttaaccttc accagcgtgc gaccctggat ctggttatta
     8401 atgatggcct cggcaaagtt cggtgcctct gacagagata tctcttttgc cgcctgggta
     8461 tagaatgatt ccggtaaatc ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt
     8521 ggcgtcatta ctgaatccac cccttgcaaa cggacattac gcagaataaa tggcatgacc
     8581 gtggttggca gagtaaaacc acccgccaga ccacaggccg ccacgcagcc gccgtaattc
     8641 atttgcgcta gcacttttgc cagcactttg tcgccaacag tgtcaattgc cccagcccag
     8701 acttgttttt ccagaggacg ggattcggca aactcatcac gagggagaat acggctggca
     8761 cctaaacttt tcagatattc atgggtactt tcgcgaccgg aaacggcaac gacctgataa
     8821 cccaacttat gcagcagcgc cacggcggta ctgccgacgc caccactggc acccgtcacg
     8881 acaatctccc cgtcctgcgg gcgaacaccg gcatcttcca gcgccatcac acacagcatg
     8941 gcggtaaaac cggcagtacc gataatcatt gctttacgcg cgtccagccc ttgcggcatg
     9001 gcaaccagcc agtcaccttt cactcgcgcc tgctccgcca gcccacccca gtggttttca
     9061 ccaacgcccc agccagtgag taacacctcc tgaccggcat gaaaacgcgg atcttcgctg
     9121 gtgcgtacag ttccggcaaa atcgatccca ggaatcatcg gaaaattacg gatgattttt
     9181 cccttaccgg taatcgccag cgcatcttta tagttcaggc tcgaccagtg aacatcgacc
     9241 gtgacatcgc cctccggcag gcgactttcg tccagagtct gtactgatgc gagagttttg
     9301 ccgtcctgct gttctaaaag taacgcctgc ataagtggtc ctcatgtgca tgatggattg
     9361 gaaaataatt tctgaagact atactcgcta atggaaaagc aaagccgatg aagcgcaata
     9421 aattgcgaga taaatctgat ttgctagtat gcccgcttcc tcactatcgg agttaacaca
     9481 aggatgagat taacgacgaa attttcggcc tttgttacgc tgctcaccgg gttaacaatt
     9541 tttgtgactt tgctgggctg ttcgctaagt ttctacaacg ccattcagta taagtttagt
     9601 catcgcgttc aggcggtggc gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc
     9661 gcattaaggc cacaaattac cgaattaatg gtgtcagcag atatcgttcg tgtagacctg
     9721 ctgcatggtg ataaacaggt ttataccctg gccagaaatg gtagttatcg tccggttggc
     9781 accagcgatc tgtttcgtga actgagcgtt ccgttgataa agcatccggg gatgtcgttg
     9841 cgtctggttt atcaggatcc gatgggcaac tatttccatt cgttgatgac caccgcgccg
     9901 ctcacggggg cgattggctt tatcattctt atgctctttc tggcggtacg ctggttacaa
     9961 cggcaacttg ccgggcaaga attgctggaa acccgggcta ctcgtatctt aaacggtgag
    10021 cgtggctcta atgtgttggg aaccatctat gaatggccgc ccagaaccag cagtgcgctg
    10081 gatacgctgc ttcgtgaaat tcagaacgca cgcgaacaac acagccgtct tgatacgctg
    10141 atccgctctt atgccgcaca ggacgtgaaa accggcctca ataaccgact ctttttcgat
    10201 aatcagttag caacgttact ggaagatcag gagaaagtag gtacccacgg gatcgtgatg
    10261 atgattcgtc tgccggattt caatatgttg agcgatacct gggggcacag ccaggttgaa
    10321 gaacagttct tcactctgac gaatctgctg tcgacattta tgatgcgtta ccctggcgca
    10381 ctgctggcgc gttaccaccg cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa
    10441 gaagcagaga gcatagccag tcagttaatc aaagccgtcg ataccttgcc gaacaataaa
    10501 atgctcgatc gcgacgatat gatccacatt ggtatctgtg cctggcgtag tggtcaggat
    10561 accgagcagg taatggaaca tgcagagtct gccacgcgta atgcgggatt gcagggtggc
    10621 aatagctggg ctatttatga tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg
    10681 cgtacgctta tcgagcaaat gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg
    10741 gttactcgcg aaggtcaggt tcatcatcgc gaactcatgt gccgcatctt cgatggtaat
    10801 gaagaggtta gctcggcgga gtatatgccg atggtcttgc agtttggctt atcggaagag
    10861 tatgaccgtc tgcaaatcag ccgtcttatt ccactattgc gttactggcc agaggaaaat
    10921 ctggcgattc aggttaccgt tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc
    10981 gatacgttaa tgcaatgtga aaaatcacaa cgaaaacgca taattattga acttgcagag
    11041 gccgatgtag gtcaacatat cagtcgttta caacctgtta ttcgtttagt gaatgcttta
    11101 ggggtacggg tagccgtcaa ccaggctggt ttgacgctgg taagcaccag ttggatcaaa
    11161 gaacttaatg ttgagttact caagctccat ccggggctgg tcaggaacat tgagaagcga
    11221 acggagaacc agctgctggt tcaaagcctg gtggaagcct gctccgggac cagcacccac
    11281 gtttacgcca ccggcgtgcg ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt
    11341 acaggcgggc aaggggattt tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa
    11401 tattcacaaa gatactcggt ttaacctgcc gtttaatccg ttttcacgta gaataacgcg
    11461 cgctgcgtct catgggagtg tgcttgtctg ctcgccagat tgttgcagca catatgcaga
    11521 tgaatgacct tacgcggttg caaacaggcg aggaatgctg ctgatgcatt aagcctttct
    11581 ggactcaggc agagatttgt aacaaaggaa acgaactgca ctaattttca ccgtagcaga
    11641 tgatttttgc gccttgtcgc tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc
    11701 gccccagctt tcaggattat cccttagtat gttgaaaaaa tttcgtggca tgttttccaa
    11761 tgacttgtcc attgacctgg gtactgcgaa taccctcatt tatgtaaaag gacaaggcat
    11821 cgtattgaat gagccttccg tggtggccat tcgtcaggat cgtgccggtt caccgaaaag
    11881 cgtagctgca gtaggtcatg acgcgaagca gatgctgggc cgtacgccgg gcaatattgc
    11941 tgccattcgc ccaatgaaag acggcgttat cgccgacttc ttcgtgactg aaaaaatgct
    12001 ccagcacttc atcaaacaag tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct
    12061 ggtttgtgta ccggttggcg cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca
    12121 gggcgctggt gcccgtgaag tcttcctgat tgaagaaccg atggctgccg caattggtgc
    12181 tggcctgccg gtttctgaag cgaccggttc tatggtggtt gatatcggtg gtggtaccac
    12241 tgaagttgct gttatctcct tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg
    12301 tgatcgtttc gacgaagcta tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg
    12361 tgaagccacc gcagaacgta tcaagcacga aatcggttcg gcttacccgg gcgatgaagt
    12421 ccgtgaaatc gaagttcgtg gccgtaactt ggcagaaggt gttccacgcg gttttaccct
    12481 gaactccaac gaaatcctcg aagcactgca ggaaccgctg accggtattg tgagcgcggt
    12541 aatggttgca ctggaacagt gcccgccgga actggcttcc gacatctccg agcgcggcat
    12601 ggtgctcacc ggtggtggcg cactgctgcg taaccttgac cgtttgttaa tggaagaaac
    12661 cggcattcca gtcgttgttg ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa
    12721 agcgctggaa atgatcgaca tgcacggcgg cgacctgttc agcgaagagt aatcggatgc
    12781 aggcagggga agcgtctgtt taccctgcct ggtctgatac gagaatacgc ataacttatg
    12841 aagccaattt ttagccgtgg cccgtcgcta cagattcgcc ttattctggc ggtgctggtg
    12901 gcgctcggca ttattattgc cgacagccgc ctggggacgt tcagtcaaat ccgtacttat
    12961 atggataccg ccgtcagtcc tttctacttt gtttccaatg ctcctcgtga attgctggat
    13021 ggcatatcgc agacgctggc ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt
    13081 caggaactgt tgctgaaaaa cagtgaactg ctgatgcttg gacaatacaa acaggagaac
    13141 gcgcgtctgc gcgagctgct gggttccccg ctgcgtcagg atgagcagaa aatggtgact
    13201 caggttatct ccacggttaa cgatccttat agcgatcaag ttgttatcga taaaggtagc
    13261 gttaatggcg tttatgaagg ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg
    13321 gtggccgtcg ctaaactgac cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg
    13381 ccaatccagg tgctgcgcaa cgatatccgc gtaattgcag ccggtaacgg ttgtacggat
    13441 gatttgcagc ttgagcatct gccagcgaat acggatattc gtgttggtga tgtgctggtg
    13501 acctccggtc tgggcggtcg tttcccggaa ggctatccgg tcgcggttgt ctcttccgta
    13561 aaactcgata cccagcgcgc ttatactgtg atccaggcgc gtccgactgc aggactgcaa
    13621 cgtttgcgtt atctgctgct gctgtggggc gcggatcgta acggcgctaa cccgatgacg
    13681 ccggaagagg tgcatcgtgt tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg
    13741 ccttcgccag acgcgatggg gccaaagtta cctgaaccgg caacggggat cgctcagccg
    13801 actccgcagc aaccggcgac aggaaatgca gctactgcgc ctgttgcgcc gacacagccc
    13861 gctgctaatc gctctccaca aagggctacg ccgccgcaaa gtggtgctca accgcctgcg
    13921 cgtgcgccgg gagggcaata gtggcgagct atcgtagcca gggacgctgg gtaatctggc
    13981 tctctttcct cattgcgctg ttgctgcaaa tcatgccctg gccggataac ctgattgttt
    14041 tccggccaaa ctgggtgtta cttatcttgt tgtattggat cctggccttg ccccatcgcg
    14101 taaatgtggg cacaggtttt gtgatgggtg ccatactgga tctgatcagc ggctcgatgc
    14161 ttggcgtacg cgtattggcg atgagcatca ttgcttacct ggtggcgctg aaataccagc
    14221 ttttccgcaa cctcgcatta tggcagcagg cgctggtcgt catgttgctt tcgctggtgg
    14281 tggatattat tgttttctgg gcagagtttt tagtgattaa cgtctctttc agaccggaag
    14341 tgttctggag tagtgtagtc aatggggtgc tctggccgtg gattttcttg ctgatgcgta
    14401 aagtccgtca gcagtttgca gtgcaataaa ggtttctatg acttctctgt atttagcttc
    14461 cggttctccg cgtcgtcagg agttacttgc gcaacttggc gtgacctttg aacgtattgt
    14521 tacgggcatt gaggagcagc gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct
    14581 ggcacgcgag aaagcacagg cgggtgtcgc gcaaacggcg caggatctcc cggtgctggg
    14641 tgcggatatt atcgttatcc tgaacggaga agtgctcgag aaaccgcgcg acgcagagca
    14701 tgcggcgcag atgttgcgca aattatcggg tcagacccat caggtgatga cggcagtggc
    14761 gttggccgac agccagcaca ttctcgattg cctggtggtc accgatgtga ctttcagaac
    14821 gttaacagac gaagacatcg cgggctatgt cgccagcggt gaaccgttag ataaagcagg
    14881 tgcatacggt attcaggggc tgggtggctg ttttgtcagg aagataaatg gcagctatca
    14941 cgccgtagtc ggcttaccgc tggttgaaac gtatgaatta ttaagtaatt ttaacgcact
    15001 gcgtgagaaa agggataaac atgacggctg aattgttagt aaacgtaacg ccttcggaaa
    15061 cgcgagtggc gtatattgat ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc
    15121 gacgcggaat agtaggcaat atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc
    15181 aggcggcttt tgtagatatt gggctggata aagccgcgtt tcttcatgca tccgacatca
    15241 tgccgcatac cgaatgtgtg gcgggtgaag aacaaaagca attcacggtg cgcgacatct
    15301 cggaactggt tcgtcagggg caagatctga tggtgcaggt ggtgaaagat ccgcttggca
    15361 ctaaaggtgc gcgcctgacc accgatatca cgctgccttc tcgctatctg gtatttatgc
    15421 caggggcttc tcacgttggg gtttcccaac gaattgaaag cgaatcagaa cgtgaacgcc
    15481 tgaaaaaagt ggtcgcagag tattgcgacg agcagggcgg gtttatcatc cgcaccgcag
    15541 cggaaggggt tggcgaggct gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga
    15601 ccaaagttat ggagcgtaaa aagcgcccgc agacccgtta tcagctgtac ggcgaactgg
    15661 cgctggcgca gcgtgttctg cgtgattttg ccgacgccga actggaccgc attcgcgttg
    15721 actcacgcct gacttacgaa gcattgctgg agtttacctc ggagtacatt cccgagatga
    15781 ccagcaagct ggagcattac accggacgcc agccgatttt cgatctcttt gatgtcgaaa
    15841 acgaaatcca gcgagcgttg gaacgcaaag tagaactgaa atccggtggt tatctgatta
    15901 tcgaccagac cgaagcgatg accaccgtgg acatcaatac cggagcgttt gtcggtcatc
    15961 gcaatctgga cgacactatt ttcaatacca atattgaagc gacgcaggct atcgctcgcc
    16021 agttacggtt gcgtaatctg ggcgggatta tcattattga tttcatcgat atgaataatg
    16081 aagatcaccg ccgccgcgtg ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga
    16141 aaaccagcgt taatggtttt tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc
    16201 gcgaaagcat tgagcacgta ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg
    16261 tgaaaaccgt ggaaacggta tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg
    16321 cttacgactc cgaccgtttc ctggtctatg cttctccggc agtagccgaa gccttgaaag
    16381 gcgaagagtc acactcgctg gcggaagtgg aaattttcgt tggcaaacag gttaaagtac
    16441 aaattgaacc gctctataac caggagcagt ttgacgtcgt aatgatgtaa acagatgctg
    16501 ggccgccatc cggcaaaggg tttttgagtc acatttttag cagacaagga gtgacgggtg
    16561 aggcgattgc cggggatttt actgcttact ggagccgcgc tcgttgtgat cgctgccctg
    16621 ctggttagcg gcctgcgtat tgctttaccg catcttgacg cctggcgtcc ggaaatcctc
    16681 aacaaaatag aatccgcgac tggcatgccg gtagaagcca gtcagctctc agccagctgg
    16741 cagaattttg gcccgacgct tgaagcacac gacatccgtg cagaactaaa agatggcggc
    16801 gaattttcgg ttaaacgcgt tactctggcg ctggatgtct ggcagagcct gttacatatg
    16861 cgctggcagt ttcgcgacct cactttctgg cagctgcgct ttcgcaccaa cactcctatc
    16921 accagcggtg gtagcgatga cagtctggaa gccagtcaca tcagcgatct gtttcttcgt
    16981 caatttgacc atttcgatct ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag
    17041 cgcgccgagc tggcgatccc acaactcacc tggctgaacg atccacgtcg acaccgtgcg
    17101 gaaggcctgg taagcctctc cagccttacc ggacagcacg gcgtgatgca ggtgcgcatg
    17161 gatttgcgcg atgatgaggg gttgttaagc aatggtcgcg tctggctaca ggcggatgac
    17221 atcgacctga agccgtggct cggtaaatgg atgcaggaca atattgctct ggaaacggca
    17281 cagttctccc ttgaaggctg gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc
    17341 tggctgaaac agggcggtgc cagctggttg ggcgagaagc aaacgcatac gctgtcggtg
    17401 gataatctga ccgcgcatat tacgcgtgaa aatccgggct ggcagttctc tattcccgat
    17461 acacggatca cgatggacgg caaaccctgg ccgagcggag cattgacgct ggcctggata
    17521 ccggaacagg acgttggcgg caaagacaat aaacgcagtg acgaactccg gattcgcgct
    17581 agtaatctgg agctggcagg cctggagggc atacgcccgc tggccgcgaa actttcacct
    17641 gcactgggtg atgtttggcg ctccacacaa ccgagcggca agattaacac tctggcgctg
    17701 gatatcccgc ttcaggcggc agacaagacc cgttttcagg catcgtggag cgatctggcc
    17761 tggaagcaat ggaaattatt accgggtgcg gaacacttct ccgggacgct ttccggcagc
    17821 gttgaaaatg gtttgcttac cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta
    17881 ttccgtgcgc cactggaaat cgccgacggc caggcaacta taagctggct gaacaatgac
    17941 aaaggtttcc agctggatgg gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc
    18001 ggcggttttc gttacctgca acctgctaac gatgaaccct ggctgggtat tctggctggc
    18061 atcagtaccg atgatggttc acaagcctgg cgctatttcc cggaaaactt gatgggtaaa
    18121 gacctggttg attatttaag tggtgcgatt cagggcggtg aagcggataa cgcgacgctg
    18181 gtttatggtg gcaatccgca actcttcccc tataaacaca acgaaggtca gtttgaagtg
    18241 ctggtgccgc tgcgcaacgc gaagtttgcc ttccagccgg actggcctgc attaactaac
    18301 cttgatattg aactggactt tattaacgac ggtttatgga tgaaaaccga tggcgttaat
    18361 ctgggcggcg tgcgcgcgag taatctcacc gcagtgatcc ctgactactc aaaagaaaaa
    18421 ctgctgattg acgctgacat taaaggtccg ggtaaagccg ttggccctta ctttgatgag
    18481 acaccgctga aagattctct gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg
    18541 aatgctcgct tacatcttga tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa
    18601 gtgacgctgc gtaataacag tctgtttatc aaaccactcg acagcaccct gaaaaatttg
    18661 agcggtaaat tcagctttat caatggcgat ctgcaaagtg aaccactgac agcaagctgg
    18721 tttaatcagc cgttgaacgt ggatttttcc accaaagaag gggcaaaagc ctaccaggtt
    18781 gcggtgaatc tcaacggtaa ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg
    18841 aacgaagcat tgagtggcag cgtggcgtgg gatggtaaag tgggcattga tctgccttat
    18901 catgctggcg cgacctataa cgtagagctg aacggcgatc tgaataatgt gagtagtcac
    18961 ttaccttcac cgttagccaa acctgcgggt gaaccactag cggtaaacgt taaggttgat
    19021 ggcaatctca acagctttga attaaccgga caggctggtg cggataacca tttcaatagc
    19081 cgctggttgc ttggtcaaaa gctgacgctc gatcgtgcta tttgggcggc agacagtaaa
    19141 acgctcccgc cgttgccgga acaaagtggc gttgaactta atatgccgcc gatgaatggt
    19201 gccgagtggc tggccctgtt ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt
    19261 ttcccacaac acataacgtt acgtacgcct atgttgtcac tgggaaatca gcaatggaat
    19321 aacctgagta ttgtttcgca accgacggca aatggcaccc tggttgaggc gcaagggcgt
    19381 gaaatcaacg ccacgctagc gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat
    19441 ctttattaca acccgagcgt ggcgaaaact cgtggtgatt caacgccgtc atcacctttc
    19501 ccgacaacgg agcgcattaa cttccgtggc tggccggacg cacaaatacg atgcacagag
    19561 tgctggttct gggggcaaaa attcggtcgc attgacagtg atatcaccat ttctggcgat
    19621 acgttaacgc tgaccaatgg actgattgat actggtttct cgcggcttac tgccgatggt
    19681 gaatgggtta ataatccggg gaatgaacgt acctcgctga aaggaaaact gcgtgggcag
    19741 aaaattgatg ccgccgcaga attttttggt gtcacgacgc ccatacgcca gtcgtcattt
    19801 aatgtggatt acgatttaca ctggcgcaaa gcaccctggc agccagatga agcgacgttg
    19861 aatggcatca ttcatactca actgggtaaa ggcgaaatta ccgaaatcaa taccggacat
    19921 gccgggcaat tgctgcgctt attgagcgta gatgccctga tgcgtaagct gcgttttgat
    19981 ttcagagaca cttttggcga agggttctat tttgactcca ttcgcagcac cgcgtggatt
    20041 aaagacggcg ttatgcacac cgacgacacg ctggtggatg gcctggaggc ggatatcgcc
    20101 atgaaagggt cggtaaatct ggtacgtcgc gacctgaata tggaagcggt tgtcgcacca
    20161 gagatttctg cgacggtggg cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg
    20221 gcagtgtttg ccgccagtaa agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc
    20281 tatcacattt cgggtccgct ggacgatccg caaatcaacg aagtgttgcg ccaaccgcgt
    20341 aaagaaaaag cgcaatgatt tgacgagggc gcgtaattgc cccaatctca tatgataatc
    20401 gttgccaaag gccaacgagc cggaacataa ctgtaggccg gatagggcgt tcacgccgca
    20461 tccggcagcc gtaaaaaatc ctctactgca gtaactaacg agtagcaaaa acgatgagtc
    20521 tgaacctggt aagtgaacaa ttgctagcgg cgaacggcct gaaacatcag gacttgttcg
    20581 cgatcctcgg tcaactggcc gaacgtcgcc ttgattatgg cgatctctat tttcagtcga
    20641 gctatcacga atcttgggtt ttagaagacc gcattattaa agatggttct tacaacatcg
    20701 atcagggcgt tggcgtgcgt gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc
    20761 aaatcagcct gctggcgctg gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata
    20821 gtggtgatgg caaagtacag acgctgggcg cggtagagca tagcccgttg tatacctcgg
    20881 tagatccgct gcaaagcatg agccgtgaag agaagctgga tatcctgcgt cgcgtcgata
    20941 aggttgctcg cgaagcggac aagcgcgtac aggaagtgac tgccagcctc agtggtgtct
    21001 atgaattaat tctggttgcg gccaccgacg gcacgctagc ggcggatgtc cgtccactgg
    21061 tgcgtctttc cgtgagcgtt ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg
    21121 gcggcggcgg tcgttttggt tatgagttct tccttgccga tcttgacggc gaagtccgtg
    21181 cggatgcatg ggcaaaagaa gcagtgcgta tggcgctggt caatctttct gccgttgctg
    21241 caccagcggg caccatgccg gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg
    21301 aagcggtagg tcacggtctg gaaggcgact tcaaccgccg tggcacttca gtatttagtg
    21361 gacaggtcgg ggagctggtg gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg
    21421 tcgatcgccg tggttcggtg gcgattgatg acgaaggtac gccaggccag tacaacgtgc
    21481 tgattgagaa cggcattctg aaaggctaca tgcaggataa actcaacgcg cgtttgatgg
    21541 ggatgacgcc gactggcaac ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta
    21601 tgaccaacac ctatatgctg ccgggtaaat cgaccccgca agaaattatt gaatccgttg
    21661 agtacggtat ctatgcaccg aactttggtg gcggtcaggt ggatatcacc tccggcaaat
    21721 tcgttttctc cacttcagaa gcatatctga ttgaaaacgg taaagtaacg aagccggtga
    21781 aaggcgcaac gttgattggt tccggtatcg aaaccatgca gcagatttcg atggttggca
    21841 acgacctgaa actggataac ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg
    21901 ttggcgtggg ccagccaacg ttgaaagtcg ataacctgac tgttggcggt actgcgtaat
    21961 aatttcatta ttttcagaag gataattaat ctttctacgt gcacgaacgg tcccctcgct
    22021 cctctggggt tagggtgagg ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa
    22081 tatgaattac ttctctttcc cgcgcccgtg catctcctga aacaatttac cgacctcaac
    22141 aaaataatcc gtcagcgagt tgatcacgac ctgtaccttc agcggcagct tatctttttc
    22201 ggtatataac gcataaaccg ggcgtggatc tgactgataa cgcggcagca ggatctccag
    22261 ctccccacga ttgatctcgt tgatcaccca catcagcggc acgtaggcga tcccggcacc
    22321 cgccgttagc cagcgcacca gcgtcatcgg atcattagtc acaaatctcc cttgcgggat
    22381 caggcgagtc gagatccctt ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct
    22441 gtattcaagc catgaatggc tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt
    22501 gagatagctt ttcgcggcgc acaccaccat tggcatcgcg ccaagacggc gggaaaacag
    22561 gctggaatcc tgcaacgcgc cgacgcggat caccacatcc agaccgtcgg caatcaggtc
    22621 gggggctgga attccggtaa ccagattggc gctcaaacct gggtattctt tcagcatttt
    22681 ggctgtcagc ccggcgagaa cattttgtgc catagttgaa gaacagccaa tgcgtagcgt
    22741 cccgatgggg gtgttattga aggcatacag ttgctcatga acatcctgca cttcatgaag
    22801 catacgacgg cagccctggt agtaaattct accggcttcg gtcaggccaa tgctgcgtgt
    22861 gctacggttt aacagcttta cctgcaactc atcttccagt tttgacaccg tctgactgat
    22921 ggacgaaacg ctcatctgta gctgtctggc ggcggcggta aaagagccaa attcaactac
    22981 tttggcaaac accgacatgc gttttagtcg ttccattatt cactctgact taaaagtgat
    23041 ttagatcaca taatatagat aacagcataa cagttacgct aatatattaa atatcaatct
    23101 acagcaatgt tgctctcgcc cggctttcca tgcccttctc tgcggcgaca gatgctgaaa
    23161 ataataacgc ctgctctctc tatacaacca aggtcaacat gagtctgttt cccgttatcg
    23221 tggtgtttgg gctgtccttc ccaccgatat tttttgaatt gcttttatca ctggcgattt
    23281 tctggctggt gcgccgggta cttgtgccaa caggtatcta cgactttgtc tggcatccgg
    23341 cgttgttcaa caccgcgctc tattgctgct tgttttattt gatatcgcga ctgttcgttt
    23401 gaggttgaag tgaaaacact aataagaaaa ttctcccgta cggccatcac ggtcgtatta
    23461 gtcattctgg ccttcatcgc aatttttaat gcctgggtct attacaccga atccccctgg
    23521 acgcgtgacg cgcgctttag cgctgacgtc gttgcgatcg cgccggacgt ttctggactc
    23581 attacccagg tgaatgttca tgataaccag ctggtgaaaa aaggacagat actgttcacc
    23641 atcgaccagc cgcgctatca aaaggcgctt gaggaagcgc aagccgatgt tgcttattat
    23701 caggtactgg cacaggagaa acgccaggag gccggacgtc gtaaccgtct cggtgtgcag
    23761 gcgatgtctc gcgaagagat cgaccaggct aacaacgtac tacaaacggt tctgcatcag
    23821 ttagcgaaag cgcaggcgac ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc
    23881 cgagcgccag cagatggctg ggtgaccaac ctcaacgtct ataccggtga gtttattact
    23941 cgaggatcaa cggcggttgc gctggtgaaa cagaactcct tctatgtact ggcctatatg
    24001 gaagaaacta agctggaagg ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc
    24061 agtaacaaag tgctgaaagg gactgttgat agtgttgccg ctggggtcac caacgccagc
    24121 agcacgcgtg acgacaaagg gatggcgacc atcgactcta accttgaatg ggtgcgtttg
    24181 gcgcaacgtg ttccggttcg tattcgtctc gacaaccagc aagagaacat ctggcctgcg
    24241 ggcaccactg ctacagtggt ggtcactggc aaacaagatc gcgacgaaag ccaggattcg
    24301 ttcttccgta aaatggccca tcgcctgcgt gagtttggtt aatcacgatg ggtattttct
    24361 ccattgctaa ccaacatatt cgctttgcgg taaaactggc gaccgccatt gtactggcgc
    24421 tgtttgttgg ctttcacttc cagctggaaa cgccacgctg ggcggtactg acagcggcga
    24481 ttgttgccgc cggtccggcc tttgctgcgg gaggtgaacc gtattctggc gctattcgct
    24541 atcgtggctt tttgcgcatc atcggcacat ttattggctg tattgccgga ctggtgatca
    24601 tcattgcgat gatccgcgca ccattattga tgattctggt gtgctgtatc tgggccggtt
    24661 tttgtacctg gatatcctcg ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg
    24721 gttataccgc gctgatcatt gtgatcacca ttcagccgga accattgctt acgccgcagt
    24781 ttgccgtcga acgttgtagc gagatcgtta tcggtattgt gtgtgcgatt atggcggatt
    24841 tgctcttttc tccgcgatcg atcaaacaag aagtagatcg agagctggaa agtttgctgg
    24901 tcgcgcaata tcaattaatg caactctgta tcaagcatgg cgatggtgaa gttgtcgata
    24961 aagcctgggg cgacctggtg cgacgcacca cggcactaca aggcatgcgc agcaacctga
    25021 atatggaatc ttcccgctgg gcgcgggcca atcgacgttt aaaagcgatc aatacgctat
    25081 cgctgacgct gattacccaa tcctgcgaaa cttatcttat tcagaatacg cgcccggaat
    25141 tgatcactga tactttccgc gaattttttg acacgccggt agaaaccgcg caggacgtcc
    25201 acaagcagct caaacgcctg cggagagtta tcgcctggac cggggaacgg gaaacgcctg
    25261 tcaccattta tagctgggtc gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta
    25321 tcagtaacac aaaaatcaac gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa
    25381 aagtagagtc agccgaacgt catcatgcaa tggttaactt ctggcgaacc acactttcct
    25441 gcattctggg cacgcttttc tggctgtgga cgggctggac ttccggcagt ggtgcaatgg
    25501 tgatgattgc ggtcgtgacg tcactggcaa tgcgtttgcc gaatccacgc atggtggcga
    25561 tcgactttat ctatgggacg ctggccgcgc tgccgttagg gctgctctac tttttggtga
    25621 ttatccctaa tacccaacag agcatgttgc tgctgtgtat tagcctggca gtgctgggat
    25681 tcttcctcgg tatagaagta cagaaacggc gactgggctc gatgggggca ctggccagca
    25741 ccataaatat tatcgtgctg gataacccga tgactttcca tttcagtcag tttctcgaca
    25801 gcgcattagg gcaaatcgtc ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg
    25861 ataaatcgcg cgacaggact ggacgtgtac tgcttaatca gtttgtttct gccgctgttt
    25921 ccgcgatgac taccaatgtg gcacgtcgta aagagaacca cctcccggca ctttatcagc
    25981 agctgttttt gctgatgaat aagttcccag gggatttgcc gaaatttcgc ctggcgctga
    26041 cgatgattat cgcgcaccag cgcctgcgtg atgcgccgat cccggttaac gaggatttat
    26101 cggcgtttca ccgacaaatg cgccgcacag cagaccatgt gatatctgcc cgtagcgatg
    26161 ataaacgtcg tcggtacttt ggccagttgc tggaagaact ggaaatctac caggaaaagc
    26221 tacgcatctg gcaagcgcca ccgcaggtga cggaaccggt tcatcggctg gcggggatgc
    26281 tccataagta tcaacatgcg ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg
    26341 gttttttcat ggctatactt agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg
    26401 caacagatga atatttatgc ctttgatttt gatgagattg agagtcagga ggatttttat
    26461 cgtgacttta gccaaacctt tggtctggct aaagataagg tacgcgatct cgactcacta
    26521 tgggatgtgt taatgaacga tgtcctgccg ctaccacttg agattgaatt tgttcatctg
    26581 ggagagaaaa cgcgtcgccg ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa
    26641 gagctggaag ggcatttgcg ttttaatgtt cgtcattagc gcaaaaaaaa gcccccgaac
    26701 cgggggcaat atcgtcggac aagacgatga gggtttattt gtacagttca gccgtagcgt
    26761 gccaggtgtc accgctacga gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg
    26821 cttttttgtt cagcatttca cgtatatcca ttggcgaaga cgccacacca cttacggata
    26881 cggtcccgat tgcttcacga ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg
    26941 caccgaaaga gagaacagaa agtacgctta atgcagctac agtggttttg attttcatga
    27001 tttttacctc gacataatct tttagctggg cctttgtttc gtgacccata tcacaaaatc
    27061 aagtatacac taattattga actaattaat accacgctaa ctgtttcagt tgatactaag
    27121 tgttaaaaat gtgaatttta ataacaaaaa agtataaaaa aacacggtta tttattatta
    27181 attatagtat tttcaattag ataatgaatt ttgcataatg tgcttttttt accgtatgta
    27241 ttcattttgt gaatgatgtg tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag
    27301 aaagaagggg aatgagaggg ataagcaaca ttttccccgc cgccagaagc gacggggcag
    27361 agattaaagc tcctggtcga acagctctaa aatcgcttcg tacaggtctt tgacggtgaa
    27421 accgttagca ggggtggtaa agatggtgtc atcgccagcg atggtgccca gaataccttc
    27481 tgctttgccc agtgagtcca gcaggcgagc aattaactgc gccgcgccag ggctggtatg
    27541 aatcacgaca actgcatcgt tgtagtcgat atccagcacc agattcttca atggactgga
    27601 ggtggttggt acacccagtt cagctggcag gcagtaaacc atttccattt tggcattgcg
    27661 tgtacgtaca gcaccaaact tggtcaacat ccgcgagact ttagactgat taatattgtc
    27721 aaagccttgc tcctgcaacg cggcgacgat ttcgccctgg gagctaaatt tctcttcttt
    27781 aagtaatgct ttaaatgctt taactagttc ttcttgctta gccgagcttc gcataagtca
    27841 cccgatatgg tggttgatac aacattattg tgcatacaga tgaattttta tgcaaacagt
    27901 cagccctgaa gaaggctgaa ataatgttat gaaagagcgg gattttatca aatttcgtta
    27961 ttgagaaaca tgcctgcgtc acggcatgca aattctgttt aaaagtaaat taattgttat
    28021 caaattgatg ttgttttggc tgaacggtag ggtatattgt caccacctgt tggaatgttg
    28081 cgctaatgca taagcgactg ttaattacgt aagttaggtt cctgattacg gcaattaaat
    28141 gcataaacgc taaacttgcg tgactacaca ttcttgagat gtggtcattg taaacggcaa
    28201 ttttgtggat taaggtcgcg gcagcggagc aacatatctt agtttatcaa tataataagg
    28261 agtttaggat gaaagtcgca gtcctcggcg ctgctggcgg tattggccag gcgcttgcac
    28321 tactgttaaa aacccaactg ccttcaggtt cagaactctc tctgtatgat atcgctccag
    28381 tgactcccgg tgtggccgtc gatctgagcc atatccctac tgctgtgaaa atcaaaggtt
    28441 tttctggtga agatgcgact ccggcgctgg aaggcgcaga tgtcattctt atctctgcag
    28501 gcgtagcgcg taaaccgggt atggatcgtt ccgacctgtt taacgttaac gccggcatcg
    28561 tgaaaaacct ggtacagcaa gttgcgaaaa cctgcccgaa agcgtgcatt ggtattatca
    28621 ctaacccggt taacaccaca gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt
    28681 atgacaaaaa caaactgttc ggcgttacca cgctggatat cattcgttcc aacacctttg
    28741 ttgcggaact gaaaggcaaa cagccaggcg aagttgaagt gccggttatt ggcggtcact
    28801 ctggtgttac cattctgccg ctgctgtcac aggttcctgg cgttagtttt accgagcagg
    28861 aagtggctga tctgaccaaa cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag
    28921 ccggtggcgg gtctgcaacc ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc
    28981 tggttcgtgc actgcagggc gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg
    29041 gtcagtacgc ccgtttcttc tctcaaccgc tgctgctggg taaaaacggc gtggaagagc
    29101 gtaaatctat cggtaccctg agcgcatttg aacagaacgc gctggaaggt atgctggata
    29161 cgctcaagaa agatatcgcc ctgggcgaag agttcgttaa taagtaattg attagcgaat
    29221 aataaaaaac cggagcacag actccggttt ttgttttgag cactcgactt aattggttgc
    29281 cggatattcc tgaatggtga cctgcagcgt taactgctta tcatcacgca tcactacaac
    29341 cgggatcacc gaaccagggc gaatttccgc cacctgatcc atcgtctcca gagcagagat
    29401 ggccggtttg ttatccaccg aaataatcag atcgttgacc tgaatacccg cattcgccgc
    29461 tgggccgtca ggtgacactt cattaaccac gatcccttgc agttgatcta taccaccgct
    29521 ctgcgcgtgc agtggtgcga tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg
    29581 accatcgcgg atcagcttat ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc
    29641 gataccttcc ggcgtttcgc catcgttact cttatcaaac gacagcgtgt taatgcccat
    29701 cagttctccc agcgagttca ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc
    29761 ggtttgtagg aagttttgcc gcccggtcgg gttcagaccg attcgacccg tggcactaat
    29821 aatcccttgg gtaatggtct gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc
    29881 gccaatgtgc ggtacgcgac gtgcattaat tggaatggta ggtaaaccgc cagtggcatt
    29941 aattttaagt accgccagat cggttagaga gtcagatccc accagcaatg cttcaaatac
    30001 acgtccatcc tgtaaggcga cgatgatctg atcggcgtcg ttgatgacgt gtttattggt
    30061 gatgatataa ccgcgttgat ccatgattac accggatccc agggtgcgga tctcaagctg
    30121 gttgtgagag ttggtgttca aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg
    30181 gcgaaccgcc agattatagc tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga
    30241 aagcgggtta aggctgcgca gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa
    30301 tccaatcgca acggaacgta agagcttcac aaacatgatg gaggcgtcat taaaaaaggg
    30361 aacggcagca gcataccacg agttaaccgg acatcacacg tcagcctgat gcccggttta
    30421 cgacattaac gcatcagcag atagatgctt tcattgccgc gtacaatttg cagggcgatg
    30481 atggccggtt ttgccgccag cactttacgc atttcagcaa tcgagttcac ccgatcgcgg
    30541 ttgacgccaa tgatcacatc gtctttttgc aagccagcct gagcagctgg gcttcctttg
    30601 acaacttcat ctatcttaat acctttgccg ccatctttta gctgaccatc gctcaacgtt
    30661 gcaccttcca gcgctggcgt gatcatttca gcgctggccg acgaagaggt gctggtatca
    30721 agcgtcactt ctacttccag tggtttgccg ttacgcagca ggccaagctt cactttcgtg
    30781 cccggctctg tagtcgcgat acgagagcgc aactcagcaa agctattcag cggtttgccg
    30841 ttgaggctgg taataatatc gcccgctttg acgcccgctt tcgctgagcc agaacttggc
    30901 aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt tgaaggcttt ggcgatatcg
    30961 gcactcatct cggtgccttt gatgcctaac aaaccgcgtt tgatttcacc aaagtcgata
    31021 agctgctgcg ccagtgttcg cgccatatta ctggggatgg caaatccaat cccgacgctc
    31081 ccgccgccag gcgcaaggat tgcagtgttg atgccaatta actcaccgtt aaggtttaat
    31141 agtgcaccgc cggagttacc gcggttaatg gaagcatctg tctggataaa gttttccaga
    31201 ccttcaagat tcaacccgct gcggcctaat gcggaaacaa tgccagaggt ggcggtttgc
    31261 ccaaggccaa atgggttacc gaccgctacg gcaaaatcac cgacgcgcaa tttatcggag
    31321 tcggcaatag cgatttgcgt taatttgctc gggttttgaa tttgtaacag ggcgatatcg
    31381 ctctggtcat cgctaccaat cagttttgca tcaaactcgc gcccatcatt gagctgaata
    31441 ctgattttct gtgcctgatt aatcacatgg ttgttggtca gcacatagcc tttactggcg
    31501 ttgatgatga caccggaacc taaaccttcg aagggttgtg caggttgatc cggtaaatca
    31561 tcaccaaaaa actttttgaa ttcttccggg attttctgtc cctgactggc cgttccttcc
    31621 acccgtacgc tcaccactgc cggaagcact ttttccagca ttggagccag actggggaga
    31681 ggggcctgat cggcaacctg gcctagaatc gacgcaacgg cctgaaatga cgccgagaga
    31741 gttaacccgg cacttaacgc taatgcactc aacagctggg tttgtttttt cattattcct
    31801 gctctcgtac ctgaatgata agaaaagaga ttcaaaacag tttgatgtta ttgaattttc
    31861 agacggctaa caatgagaca ccagattaaa tgatagtcgg ggagggagag aagaggaggg
    31921 cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg cgccagtacg cagcaggccg
    31981 gatgcgcctt cagaatagtc gcgaggcatc tgcaccggtg cctgatcgtt gctggcttca
    32041 gactctgcca gacgattacg gaacgggttt gcttcagcag acagttccgg cagcaggctg
    32101 ctggagcttt ttgccatgtg ctgatacagc tggcgatagt cgtgcgccat ggtatccagt
    32161 aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc gatactcgtc cagttcagct
    32221 ttattctttt ccagttcgta ctgcaacgcc tgttgctggc gtagtttacg attaccaaaa
    32281 cgcatggcca cagcaccaat aatgatgccg acgactaacc caattagcgc atattcccag
    32341 gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg ttggcttcag gctccatgcc
    32401 tacggctgat tatgccacta taaccgttaa ttccacagaa gtggaatccc gactgcatat
    32461 cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac aacggcgcac cgattattca
    32521 aggaataaca ataagatcat gcaaagcgtt accccaacat cgcaatacct gaaggcgctc
    32581 aatgaaggca gccatcaacc cgacgacgtt caaaaagagg ccgtcagccg cctggaaatt
    32641 atttatcaag aactcatcaa tagcaggcca ccagccccca ggacgagtgg gctaatggcg
    32701 cgggtcggta agctgtgggg taaacgcgaa gacacaaagc atacgccagt gcgtggctta
    32761 tatatgtggg gcggtgtagg acgcgggaaa acctggctga tggacctttt ctatcaaagc
    32821 ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt ttatgctgcg ggtgcacgaa
    32881 gagctaactg ccttacaggg gcagaccgat ccgctggaaa ttattgccga tcgctttaaa
    32941 gccgaaactg acgtgctctg ttttgacgaa ttttttgttt ctgatattac cgacgccatg
    33001 ctacttggcg gtctgatgaa agccctgttc acccgcggta ttaccctggt agcgacgtca
    33061 aatattccgc cggacgaact ttatcgaaat ggcctgcaac gtgcgcgttt tctgcctgca
    33121 atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg acgctggtgt tgattatcga
    33181 ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac tcaacgatga aacccgggcg
    33241 cagatggata aactatggtt ggcgctggcg ggggtgaaac gagaaaattc accgacgtta
    33301 gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga accagacgct ggcggtttct
    33361 tttactacgc tgtgcgtcga cgcccgcagt cagcatgact atattgcgct ctcacgcctg
    33421 tttcacacag taatgttgtt tgatgtgcca gtcatgacgc ggttgatgga gagcgaagcg
    33481 cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc atgtcaaatt agtggtgagt
    33541 gcagaagtgc cgctgtatga aatttatcag ggtgagcggc tgaagtttga gttccagcgt
    33601 tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc tgaagcgcga gcatttggcg
    33661 ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt tgaccccgac ttctctataa
    33721 tcctgcgacc ccacgttaca agaaagtttt tttcccaaaa ctttttgtgt gctggcatag
    33781 gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga acctcaacaa ttgaagacgt
    33841 ttgggtgttc accaacgtgt aactatttat tgggtaagct tttaatgaaa acttttacag
    33901 ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga cgcgaccggt aaaactctgg
    33961 gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa gcacaaagcg gaatatactc
    34021 cgcacgtaga taccggtgat tacatcatcg ttctgaacgc tgacaaagtt gctgtaaccg
    34081 gcaacaagcg tactgacaaa gtgtactatc accacaccgg ccacatcggt ggtatcaaac
    34141 aagcgacctt tgaagagatg attgctcgcc gtcctgagcg tgtgattgaa atcgcggtta
    34201 aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt ccgtaaactg aaagtttacg
    34261 cgggtaacga gcacaaccac gcggcacagc aaccgcaagt tcttgacatc taatcgggat
    34321 tataggcaat ggctgaaaat caatactacg gcactggtcg ccgcaaaagt tccgcagctc
    34381 gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa ccaacgttct ctggaacagt
    34441 acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc gctggaactg gtcgacatgg
    34501 ttgagaaact ggacctgtac atcaccgtta aaggtggtgg tatctctggt caggctggtg
    34561 cgatccgtca cggtatcacc cgcgctctga tggaatacga cgagtccctg cgttctgaac
    34621 tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt tgaacgtaag aaagtcggtc
    34681 tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta attggcttct gctccggcag
    34741 aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt atggctaaat tctgaatcag
    34801 cgtaaaaaat gggaagttgc tttttgctgc cgtctgaaag acaggtaaaa caaaaaccac
    34861 atcgccaata agggtctaag tcaactattt cagactaaag cgcatctctt tttccccatt
    34921 tccggcatcc actcaccaca aaggtcgcaa aatctggtaa actatcatcc aattttctgc
    34981 ccaaatgtcg ggtattgctc attttttgtt tgattttcga acaaagagag tcgttcctta
    35041 ttgggtaaca caacttctga ctggccacct ggccacctgg tggctggtag cagtaaaaat
    35101 tctgactata cctggaggtt ttcatggctg tcgctgccaa caaacgttcg gtaatgacgc
    35161 tgttttccgg tcctactgac atctatagcc atcaggtccg cattgtgctg gctgagaaag
    35221 gtgtaagttt cgagatcgaa cacgtggaaa aggacaatcc gcctcaggat ctgattgacc
    35281 tcaacccgaa tcagagcgtt ccgaccctgg tggatcgtga gctgaccctg tgggaatctc
    35341 gcatcattat ggaatatctg gatgagcgtt tcccgcatcc gccactgatg cctgtttacc
    35401 cggtagctcg cggtgaaagc cgtctgtaca tgcatcgcat cgaaaaagac tggtacacgc
    35461 tgatgaacac tatcatcaac ggttcagctt ctgaagcaga tgccgcacgt aagcaactgc
    35521 gcgaagaact gctggcgatt gcgccggtct tcggtcagaa gccgtacttc ctgagcgatg
    35581 agttcagtct ggtcgattgc taccttgctc cgctgctgtg gcgtctgccg caactgggca
    35641 tcgagttcag cggcccgggt gcgaaagagc tgaaaggcta tatgacccgc gtctttgagc
    35701 gtgactcttt ccttgcttct ttaactgaag cagaacgtga aatgcgtctg ggccggagtt
    35761 aatctgtatg gatttgtcac agctaacacc acgtcgtccc tatctgctgc gtgcattcta
    35821 tgagtggttg ctggataacc agctcacgcc gcacctggtg gtggatgtga cgctccctgg
    35881 cgtgcaggtt cctatggaat atgcgcgtga cgggcaaatc gtactcaaca ttgcgccgcg
    35941 tgctgtcggc aatctggaac tggcgaatga tgaggtgcgc tttaacgcgc gctttggtgg
    36001 cattccgcgt caggtttctg tgccgctggc tgccgtgctg gctatctacg cccgtgaaaa
    36061 tggcgcaggc acgatgtttg agcctgaagc tgcctacgat gaagatacca gcatcatgaa
    36121 tgatgaagag gcatcggcag acaacgaaac cgttatgtcg gttattgatg gcgacaagcc
    36181 agatcacgat gatgacactc atcctgacga tgaacctccg cagccaccac gcggtggtcg
    36241 accggcatta cgcgttgtga agtaatacaa aacaggccca ggcggcctgt tttgtctttt
    36301 taatgactta ataa
//
LOCUS       DBKEKI010000027        36166 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.027, whole genome shotgun sequence.
ACCESSION   DBKEKI010000027 DBKEKI010000000
VERSION     DBKEKI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.027"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(37..2712)
                     /gene="adhE"
                     /locus_tag="ACUQPX_001323"
                     /gene_synonym="adhC"
     CDS             complement(37..2712)
                     /gene="adhE"
                     /locus_tag="ACUQPX_001323"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HHV9056083.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3105..3158
                     /gene="ychE"
                     /locus_tag="ACUQPX_001324"
     CDS             3105..3158
                     /gene="ychE"
                     /locus_tag="ACUQPX_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HHV9056084.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            3189..3836
                     /gene="ychE"
                     /locus_tag="ACUQPX_001325"
     CDS             3189..3836
                     /gene="ychE"
                     /locus_tag="ACUQPX_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HHV9056085.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            4574..6205
                     /gene="oppA"
                     /locus_tag="ACUQPX_001326"
     CDS             4574..6205
                     /gene="oppA"
                     /locus_tag="ACUQPX_001326"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HHV9056086.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLEKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTAGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            6291..7211
                     /gene="oppB"
                     /locus_tag="ACUQPX_001327"
     CDS             6291..7211
                     /gene="oppB"
                     /locus_tag="ACUQPX_001327"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HHV9056087.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            7226..8134
                     /gene="oppC"
                     /locus_tag="ACUQPX_001328"
     CDS             7226..8134
                     /gene="oppC"
                     /locus_tag="ACUQPX_001328"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HHV9056088.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAISGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            8146..9159
                     /gene="oppD"
                     /locus_tag="ACUQPX_001329"
     CDS             8146..9159
                     /gene="oppD"
                     /locus_tag="ACUQPX_001329"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HHV9056089.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            9156..10160
                     /gene="oppF"
                     /locus_tag="ACUQPX_001330"
     CDS             9156..10160
                     /gene="oppF"
                     /locus_tag="ACUQPX_001330"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HHV9056090.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQEPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10213..10542)
                     /locus_tag="ACUQPX_001331"
     CDS             complement(10213..10542)
                     /locus_tag="ACUQPX_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HHV9056091.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(10577..12037)
                     /gene="cls"
                     /locus_tag="ACUQPX_001332"
     CDS             complement(10577..12037)
                     /gene="cls"
                     /locus_tag="ACUQPX_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HHV9056092.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            12180..12353
                     /locus_tag="ACUQPX_001333"
     CDS             12180..12353
                     /locus_tag="ACUQPX_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HHV9056093.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(12411..13664)
                     /gene="kch"
                     /locus_tag="ACUQPX_001334"
     CDS             complement(12411..13664)
                     /gene="kch"
                     /locus_tag="ACUQPX_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HHV9056094.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(13964..14260)
                     /locus_tag="ACUQPX_001335"
     CDS             complement(13964..14260)
                     /locus_tag="ACUQPX_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HHV9056095.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            14475..15203
                     /gene="tonB"
                     /locus_tag="ACUQPX_001336"
     CDS             14475..15203
                     /gene="tonB"
                     /locus_tag="ACUQPX_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HHV9056096.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(15243..15641)
                     /gene="yciA"
                     /locus_tag="ACUQPX_001337"
     CDS             complement(15243..15641)
                     /gene="yciA"
                     /locus_tag="ACUQPX_001337"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HHV9056097.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(15746..16285)
                     /locus_tag="ACUQPX_001338"
     CDS             complement(15746..16285)
                     /locus_tag="ACUQPX_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HHV9056098.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(16315..17058)
                     /locus_tag="ACUQPX_001339"
     CDS             complement(16315..17058)
                     /locus_tag="ACUQPX_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HHV9056099.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIIAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(17086..17175)
                     /locus_tag="ACUQPX_001340"
                     /pseudo
     CDS             complement(17086..17175)
                     /locus_tag="ACUQPX_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            17415..18053
                     /gene="ompW"
                     /locus_tag="ACUQPX_001341"
     CDS             17415..18053
                     /gene="ompW"
                     /locus_tag="ACUQPX_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HHV9056100.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(18113..18619)
                     /locus_tag="ACUQPX_001342"
     CDS             complement(18113..18619)
                     /locus_tag="ACUQPX_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HHV9056101.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(18666..19166)
                     /locus_tag="ACUQPX_001343"
     CDS             complement(18666..19166)
                     /locus_tag="ACUQPX_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HHV9056102.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(19252..19431)
                     /locus_tag="ACUQPX_001344"
     CDS             complement(19252..19431)
                     /locus_tag="ACUQPX_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HHV9056103.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(19812..20618)
                     /gene="trpA"
                     /locus_tag="ACUQPX_001345"
     CDS             complement(19812..20618)
                     /gene="trpA"
                     /locus_tag="ACUQPX_001345"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HHV9056104.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGIFAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(20618..21811)
                     /gene="trpB"
                     /locus_tag="ACUQPX_001346"
     CDS             complement(20618..21811)
                     /gene="trpB"
                     /locus_tag="ACUQPX_001346"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HHV9056105.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPSGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(21823..23184)
                     /gene="trpCF"
                     /locus_tag="ACUQPX_001347"
     CDS             complement(21823..23184)
                     /gene="trpCF"
                     /locus_tag="ACUQPX_001347"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HHV9056106.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARITAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(23185..24780)
                     /gene="trpD"
                     /locus_tag="ACUQPX_001348"
     CDS             complement(23185..24780)
                     /gene="trpD"
                     /locus_tag="ACUQPX_001348"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HHV9056107.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(24780..26342)
                     /gene="trpE"
                     /locus_tag="ACUQPX_001349"
     CDS             complement(24780..26342)
                     /gene="trpE"
                     /locus_tag="ACUQPX_001349"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HHV9056108.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(26364..26457)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(26434..26478)
                     /gene="trpL"
                     /locus_tag="ACUQPX_001350"
     CDS             complement(26434..26478)
                     /gene="trpL"
                     /locus_tag="ACUQPX_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HHV9056109.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            26616..27497
                     /gene="rnm"
                     /locus_tag="ACUQPX_001351"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             26616..27497
                     /gene="rnm"
                     /locus_tag="ACUQPX_001351"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0035312 - 5'-3' DNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase RNM"
                     /protein_id="HHV9056110.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            27494..28114
                     /locus_tag="ACUQPX_001352"
     CDS             27494..28114
                     /locus_tag="ACUQPX_001352"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HHV9056111.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            28215..29087
                     /gene="rluB"
                     /locus_tag="ACUQPX_001353"
     CDS             28215..29087
                     /gene="rluB"
                     /locus_tag="ACUQPX_001353"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HHV9056112.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(29116..29706)
                     /gene="cobO"
                     /locus_tag="ACUQPX_001354"
     CDS             complement(29116..29706)
                     /gene="cobO"
                     /locus_tag="ACUQPX_001354"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HHV9056113.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACRKVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(29703..30461)
                     /locus_tag="ACUQPX_001355"
     CDS             complement(29703..30461)
                     /locus_tag="ACUQPX_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HHV9056114.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            30681..31730
                     /gene="sohB"
                     /locus_tag="ACUQPX_001356"
     CDS             30681..31730
                     /gene="sohB"
                     /locus_tag="ACUQPX_001356"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HHV9056115.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(31766..32017)
                     /locus_tag="ACUQPX_001357"
     CDS             complement(31766..32017)
                     /locus_tag="ACUQPX_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HHV9056116.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            32397..34994
                     /gene="topA"
                     /locus_tag="ACUQPX_001358"
     CDS             32397..34994
                     /gene="topA"
                     /locus_tag="ACUQPX_001358"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HHV9056117.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            35204..>36166
                     /gene="cysB"
                     /locus_tag="ACUQPX_001359"
     CDS             35204..>36166
                     /gene="cysB"
                     /locus_tag="ACUQPX_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HHV9056118.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAAALRSNEEIEVMFKDIKL"
BASE COUNT         9183 a         8757 c         9160 g         9066 t
ORIGIN      
        1 gaaggggccg tttatattgc cagacagcgc tactgattaa gcggattttt tcgctttttt
       61 ctcagcttta gccggagcgg cttctttttt cgctgcagtt tcaccttcta cataatcacg
      121 accgtagtag gtatccagca gaatctgttt cagctcggag atcagcgggt aacgcgggtt
      181 agcgccggtg cactggtcat cgaatgcatc ttcagacagt ttatccacgt tcgccaggaa
      241 gtctgcttcc tgaacgccag cttcacggat agatttcgga atacccagtt cagctttcag
      301 cgtttccagc catgccagca gtttctcgat cttagcagca gtacggtcgc ccggtgcgct
      361 cagacccagg tggtcggcaa tttcagcata acgacggcga gcctgcggac ggtcgtactg
      421 gctgaatgca gtctgcttgg tcgggttgtc gttcgcattg tagcgaataa cgttacaaat
      481 cagcagggcg tttgccagac cgtgcggaat atggaactgg gaacccagtt tgtgcgccat
      541 tgagtgacat acacccagga aggcgttcgc aaatgcgata cccgcgatag ttgctgcact
      601 gtgaacacgt tcacgcgcta ccggattttt agacccttcg tggtaggacg ctggcagata
      661 ttctttcagc agtttcagtg cctgcagagc ctgaccatca gagaactcag atgccagtac
      721 agaaacataa gcttccatgg cgtgagttac tgcgtccaga ccaccgaaag cacacaggga
      781 cttcggcatg tccataacca ggttggcgtc gacaatcgcc atatccgggg tcagcgcata
      841 gtccgccagc gggtatttct gaccagtagc gtcgtcagtt acaaccgcaa acggagtgac
      901 ttcagaacct gtaccagaag tggtggtgac agcgatcatt ttcgctttca cgcccatttt
      961 cgggaacttg tagatacgtt tacggatatc cataaagcgc agcgccagct cttcgaagtg
     1021 agtttccgga tgttcgtaca taacccacat gatcttcgcg gcgtccatcg gggaaccacc
     1081 acccagcgcg ataatcacgt ctggtttgaa ggagtttgcc agttctgcac ctttacgaac
     1141 gatgctcagg gtcgggtccg cttctacttc gaagaagact tcagtttcaa cgcctgctgc
     1201 tttcagtacg gaagtgatct gatcagcata accattgttg aacaggaagc ggtcagtcac
     1261 gatgagcgca cgtttgtggc catcagtaat cacttcatcc agcgcgattg gcagggagcc
     1321 acggcggaag tagatagatt tcggaagttt gtgccacaac atgttttcag ctcgcttagc
     1381 aacggttttc ttgttgatca ggtgtttcgg accaacgttt tcagagatgg agttaccacc
     1441 ccaggaacca caacccagag tcagggaagg tgcgagtttg aagttataca ggtcaccgat
     1501 accaccctga gaagccgggg tgttaatcag gatacgagct gttttcattt tctgaccgaa
     1561 gtaagaaacg cgagccggtt ggttatcctg gtcagtgtac aggcaagagg tatgaccgat
     1621 accgcccata gcaaccagtt tctctgcttt ttctaccgcg tcttcgaaat ctttagcgcg
     1681 gtacattgcc agagtcgggg acagtttttc atgtgcgaac ggttcgcttt catcaacaac
     1741 ggtcacttca ccgatcagaa tcttggtgtt ttctggtaca gagaagcctg ccagctcagc
     1801 aattttatag gctggctgac caacgatagc cgcgttcagc gcaccatttt tcaggataac
     1861 atcctgaaca gctttcagct ctttaccctg caacagatag ccgccgtggg ttgcgaaacg
     1921 ttcacgtact gcgtcataaa cagagtcaac aacaacaaca gactgttcag aagcacagat
     1981 tacgccgttg tcgaaggttt tggacatcag tacagatgca actgcacgtt tgatatcagc
     2041 agtttcatca ataacaactg gagtgttacc cgcgcctaca ccgatagctg gtttaccgga
     2101 gctgtatgcg gctttaacca tgcccggacc accagtcgcg aggatcaggt tgatgtctgg
     2161 gtggtgcatc agtgcgttag acagttcaac agaaggttga tcgatccagc cgatcagatc
     2221 tttcggagca ccggcagcga tagcagcctg cagaacgata tcagccgctt tgttggttgc
     2281 atcttttgca cgcgggtgcg gggagaagat aatggcgtta cgggtcttca ggctgatcag
     2341 cgatttgaag atagcagttg aagtcgggtt agtggtcgga acgataccgc aaataatacc
     2401 gattggttca gcgatagtga tggtaccaaa agtgtcgtct tcagacagaa caccacaggt
     2461 tttttcatct ttataggcgt tgtagatata ttcagaagca aagtggtttt tgatcacttt
     2521 atcttcgacg atacccatgc cggattcggc aacggccatt ttcgcgagtg ggattcgagc
     2581 atctgcagca gccagagcgg cggcgcggaa gattttgtct acttgctctt gagtgaaact
     2641 ggcatattca cgctgggctt tttttacacg ctctacgagt gcgttaagtt cagcgacatt
     2701 agtaacagcc ataatgctct cctgataatg ttaaactttt ttagtaaatc atctgctcga
     2761 atacgagagt atagtcagtg cggtgatgat ttgcttaacc gatgaaaatc aaaagcttac
     2821 tcgcgctcac actcactgtg atttactaaa agagtttaaa cattagagtt attatctcta
     2881 atgcgtcact tccaggtggc gtaagcaaga ttactcactt ctgggtactg attacgtgat
     2941 ccaaatcaaa tttttgcaaa gctgatacct ttcagcatcg cttttcgcca ttatagctaa
     3001 cagttaataa attgtagtat gatttggtgg ctacattagc atgttttgca caactagata
     3061 acaataacga atgatagcaa ttttaagtag ttaggaggtg aaaaatgctg tcaaaaggcg
     3121 tattgtcagc gcgtcttttc aaccttattt atggctaaca ttatccggct tttgcttcgg
     3181 agctaaccgt gattcagacc ctttttgatt ttcccgttta cttcaaattt ttcatcgggt
     3241 tatttgcgct ggtcaacccg gtagggatta ttcccgtctt tatcagcatg accagttatc
     3301 agacagcggc agcgcgaaac aaaactaacc ttacagccaa cctgtctgtg gccattatct
     3361 tgtggatctc gctttttctc ggcgacacga ttctacaact ttttggtata tcaattgatt
     3421 cgttccgtat cgccgggggc atcctggtgg tgacgatagc gatgtcgatg atcagcggca
     3481 agcttggcga ggataaacag aacaagcaag aaaaatcaga aaccgcggta cgtgaaagta
     3541 ttggtgtggt gccattggcg ttgccgttga tggcggggcc aggggcgatc agttctacca
     3601 tcgtctgggg tacgcgttat cacagcatta gctatctgtt tggtttcttt gtggctattg
     3661 cattgttcgc tttatgttgc tggggattgt tccgcatggc accgtggctg gtacgggttt
     3721 tacgccagac cggcatcaac gtgattacgc gtattatggg gctattgctg atggcattgg
     3781 ggattgaatt tatcgttact ggtattaagg ggattttccc cggcctgctt aattaattcc
     3841 tttcaaatga aacggagctg ccatgctccg tttacttcgt cattattttt actttgttcc
     3901 cgcgcagtta tcaaaagcaa aaggaatagg taaaaatatt cttctcaaat tacagttagt
     3961 tataaggatt tccttaactg cttctcctca ccatcatgtt attttcgcca catcataatc
     4021 ctgggcttgc tgaagaataa ttgaaatgat attagtaatt ccactgcctt tggtagagga
     4081 aagtgctaaa taataatcaa ttgttaaatt attgtgcatt tcactactgg aactgtaatc
     4141 agaaaagata gacatgctta gccaatctct atttgattga attgaaagat gtttgttaag
     4201 gcatggatgc aagctataga ttctgatacg gtcaataaaa gagaatcgct taacaatttt
     4261 gcaaaatgta ttggcgagta agaaccgcat ttggtacttt ccgggcaacc gccagacgat
     4321 tctttattgg taatgagaat aattaacaat taaagagcgt cgcgaaagaa taatgtgtct
     4381 cgacagggga gacacagtac gaatcgacat aaggtgatcg tctgaatcac cagaataaat
     4441 aaagtcggtg atagtaatac gtaacgataa agtaacctga cagcagaaag tctccgagcc
     4501 tgtgcagggt cccaatccgg gattacacat gctggttaat accagtaatt ataatgaggg
     4561 agtccaaaaa acaatgacca acatcaccaa gagaagttta gtagcagctg gcgttctggc
     4621 tgcgctaatg gcagggaatg tcgcgctggc agctgatgta cccgcaggcg tcacactggc
     4681 ggaaaaacaa acactggtac gtaacaatgg ttcagaagtt cagtcattag atccgcacaa
     4741 aattgaaggt gttccggagt ctaatatcag ccgagacctg tttgaaggct tactggtcag
     4801 cgatcttgac ggtcatccag cacctggcgt cgctgaatct tgggataata aagacgcgaa
     4861 agtctggacc ttccatttgc gtaaagatgc gaaatggtct gatggcacgc cagtcacagc
     4921 acaagacttt gtgtatagct ggcaacgttc tgttgatccg aacactgctt ctccgtatgc
     4981 cagttatctg caatatgggc atatcgccgg tattgatgaa attcttgaag ggaaaaaacc
     5041 gattaccgat ctcggcgtga aagctattga tgatcacaca ttagaagtca ccttaagtga
     5101 acccgttccg tacttctata aattacttgt tcacccatca acttcaccgg tgccaaaagc
     5161 cgctatcgag aaattcggcg aaaaatggac ccagcctggt aatatcgtca ccaacggtgc
     5221 ctatacctta aaagattggg tcgtaaacga acgaatcgtt cttgaacgca gcccgaccta
     5281 ctggaacaac gcgaaaaccg ttattaacca ggtaacctat ttgcctattg cttctgaagt
     5341 taccgatgtc aaccgctacc gtagtggtga aatcgacatg acgtataaca acatgccgat
     5401 cgaattgttc cagaagctgg aaaaagagat cccggacgaa gttcacgttg atccatacct
     5461 gtgcacttac tattacgaaa ttaacaacca gaaaccgcca ttcaacgatg tgcgtgtgcg
     5521 taccgcactg aaactaggta tggaccgcga tatcattgtt aataaagtga aagcgcaggg
     5581 cgacatgccc gcctatggtt acactccacc gtatactgct ggcgcaaaat tgactcagcc
     5641 agaatggttt ggctggagcc aggaaaaacg taacgaagaa gcgaaaaaac tgctggctga
     5701 agcgggttat accgcagaca aaccgttgac catcaacctg cggtataaca cctccgatct
     5761 gcataaaaag ctggcgattg ctgcctcttc attgtggaag aaaaacattg gtgtaaacgt
     5821 caaactggtt aaccaggagt ggaaaacgtt cctcgacacc cgtcaccagg gcacttttga
     5881 tgtggcccgt gcaggctggt gtgcagacta caacgaacca acttccttcc tgaacaccat
     5941 gctgtcgaac agctcgatga ataccgcgca ttataagagc ccggcctttg acagcattat
     6001 ggcggaaacg ctgaaagtga ctgacgaggc gcagcgcaca gctctgtaca ctaaagcaga
     6061 acaacagctg gataaggatt cggccattgt tcctgtttat tactacgtga atgcgcgtct
     6121 ggtgaaaccg tgggttggtg gctataccgg caaagatccg ctggataata cctatacccg
     6181 gaatatgtac attgtgaagc actaatggca atacgtgggg caggagtgtc ctgctccacg
     6241 gtgtctgatt tttatcgcat tacagaaggc acaggccaga aggtagggca atgttaaaat
     6301 ttattctacg tcgctgtctg gaagcgattc cgacgctatt tattcttatt actatttcgt
     6361 tctttatgat gcgcctcgcg ccgggaagcc cttttaccgg cgaacgtact ttaccgccag
     6421 aagtgatggc caatatcgaa gcgaaatatc atcttaatga tccgatcatg acacagtatt
     6481 tcagctacct gaaacaactg gcgcatggcg atttcggtcc atcgtttaaa tataaagatt
     6541 attcggtcaa tgacctggtg gcatccagtt ttcccgtttc tgccaaactg ggagccgccg
     6601 catttttcct tgcggtaata ctgggtgtta gtgctggcgt tattgccgca ttaaaacaaa
     6661 acaccaaatg ggactatacc gtgatggggc tggcaatgac cggggttgtt atccccagtt
     6721 ttgtggttgc gccattatta gtcatgatat ttgcgatcat tttgcattgg ctgccgggcg
     6781 gtggctggaa tggtggggcg cttaaattca tgatattgcc gatggtggcg ttgtcactcg
     6841 cctatatcgc cagtattgcg cgtattaccc gtggctctat gattgaagta ttacactcca
     6901 actttattcg tactgcccgg gcgaaagggt tacctatgcg gcggatcatt ttacgccacg
     6961 cattaaaacc tgctctgtta cccgtgctct cctatatggg ccccgcattt gtcggcatta
     7021 ttaccggttc tatggtcatc gaaaccattt atggtttgcc ggggattggg caactgttcg
     7081 ttaatggcgc attgaaccgt gactattcct tagtgttaag cctgaccatc ctggttggtg
     7141 cgttaaccat tttgtttaat gccattgtcg atgtgctata tgcggttatc gacccgaaaa
     7201 tccgttactg atactggagc tcgcgatgat gttaagtaag aaaaacagcg agacgctgga
     7261 aaatttcagt gaaaagctgg aggtcgaagg gcgcagcttg tggcaggacg cacgtcgacg
     7321 ttttatgcat aaccgtgcgg cggttgccag tctgatagtg ctggtgctga tcgcgttatt
     7381 tgtaatcctg gcaccgatgc tttcccagtt tgcctatgac gatactgact gggcgatgat
     7441 gtccagcgcc ccggatatgg agtccggtca ctactttggt actgactcat ccggtcgcga
     7501 cctgcttgtg cgcgttgcga ttagcgggcg tatctcactc atggtcggtg ttgctgcggc
     7561 actggtggca gtggtcgtgg ggacacttta tggttcgctt tccggttatc tgggcggtaa
     7621 agtggattcg gtaatgatgc gtctgctgga aatcctcaac tccttcccat tcatgttctt
     7681 cgtcattttg ctggtgacct ttttcggcca aaacatcctg ctgattttcg tggcgattgg
     7741 catggtttcc tggctggata tggctcgtat tgtgcgtggg caaaccctga gtctgaagcg
     7801 caaagagttt attgaggcgg cacaagttgg cggtgtatcg acgccgggca ttgttattcg
     7861 ccacattgtg ccgaacgtac tcggtgtggt ggtggtctac gcatcgctac tggtgcccag
     7921 catgatcctc tttgaatctt tccttagctt cctggggttg ggtacgcaag agccgttaag
     7981 cagctggggg gcattgctga gtgatggcgc gaactcgatg gaagtctctc catggttact
     8041 gttgttccca gcgggattcc ttgtggtgac actgttttgt ttcaacttta tcggcgatgg
     8101 cttgcgtgat gccctcgacc cgaaagatcg ttaaggagtg cagccatgag cgtaattgaa
     8161 actgcaactg tgccgctcgc acaacaacag gctgacgcac tgctgaacgt gaaagatttg
     8221 cgtgtcacct ttagtacccc ggacggcgac gtcacggcgg tcaatgattt aaatttttcc
     8281 ctacgtgccg gagaaacgct gggcattgta ggtgagtctg gttcgggtaa atcgcaaact
     8341 gcatttgcgt tgatgggcct gttggctgcc aacggacgca ttggcggatc ggcaaccttc
     8401 aatgggcgtg aaatcctcaa tttgccagag cgtgaactca ataaactgcg cgctgaacaa
     8461 atctcaatga tttttcagga cccaatgact tcgttgaatc cctatatgcg cgtcggtgag
     8521 cagttgatgg aagtgctgat gctgcataag aacatgagca aagctgaggc gtttgaagag
     8581 tcggtgcgga tgctcgatgc ggtaaaaatg ccggaagcac gtaaacgcat gaaaatgtac
     8641 ccgcacgaat tttctggcgg catgcgtcag cgagtcatga ttgcgatggc attgctatgt
     8701 cgacctaagt tgctgattgc ggatgaaccc actacagcgc tggacgtcac cgtacaggcg
     8761 cagatcatga cgctattgaa tgaactgaag cgggaattta atactgccat cattatgatc
     8821 acccacgatc ttggcgtggt ggcggggatc tgtgacaaag tgctggtaat gtacgccggg
     8881 cgcacgatgg aatatggcaa tgcgcgcgat gtcttttatc aacccgttca tccttattct
     8941 atcggtttgc tcaacgcggt gccgcgtctt gatgcggaag gtgaaacaat gttgaccatc
     9001 ccgggtaatc cgccaaactt gctgcgatta ccgaaaggtt gcccgttcca gccacgttgt
     9061 ccacatgcga tggaaatttg tagtagcgca ccgccgctgg aagagtttac gcctggccgt
     9121 ctgcgtgctt gctttaaacc ggtggaggaa ctgttatgaa tgctgtaact gaaggaagaa
     9181 aagtcctcct tgaaatcgcc gatcttaaag tgcactttga aatcaaagat ggcaaacagt
     9241 ggttctggca accgccgaaa acgctcaaag ccgtcgatgg tgtcactctt cgcctgtatg
     9301 aaggggaaac attaggtgtg gtaggggaat cgggatgcgg taagtccacc tttgctcgcg
     9361 ccatcatcgg tttggtcaag gcgaccgacg gtcatgttgc ctggttaggt aaagagttgc
     9421 tgggcatgaa gcccgatgaa tggcgtgccg ttcgcagtga tattcagatg attttccagg
     9481 agccgttggc atcgctaaac ccgcgtatga ccatcggcga gatcatcgct gaaccactgc
     9541 gtacttatca tccgaaaatg tcacgccagg aagttcgcga gcgcgtgaag gcgatgatgc
     9601 tgaaagtcgg gttattgcct aacctgatta accgctatcc gcatgagttc tccggtgggc
     9661 agtgccagcg tatcgggatt gctcgtgctc ttattcttga accgaagctg attatctgcg
     9721 atgagccggt ttcggcgctg gacgtgtcaa ttcaggcgca ggtggtcaac ctgctccagc
     9781 agctgcaacg tgagatggga ttgtcattaa tttttattgc tcatgacttg gccgtggtaa
     9841 aacacatttc cgatcgtgtg ttggtgatgt atctcggcca tgcagtagaa ctggggacct
     9901 atgatgaggt ctaccacaat ccactacatc cttacaccag ggcattgatg tcggcagtcc
     9961 ccatacctga tccggatctg gagaagaaca aaaccatcca gttactggaa ggggaattac
    10021 cgtcgccgat caacccgcct tccggttgtg ttttccgtac ccgttgcccg attgccggtc
    10081 cggagtgcgc caaaacacgt cctgttctgg aggggagttt cagacacgcc gtttcttgcc
    10141 tgaaagtcga tccgctttaa aaacaataag ggctgacagt tgtcagccct ttttcacgct
    10201 aaaagcgatt atttattccc gccagatgat atggcaaagt ttgtgatctt tttcgcggca
    10261 taacagaatg cgggcgaaaa catcgttgat ttcaccatct tcactgtccg ccagaccaat
    10321 caccacttcg gcaaaaaagt ccgggttcaa atcgaaatca acatgttcct gccagtcttc
    10381 cgccggatcg aataactccg cgccgccgcg ctcttcaaac tgaagattga acagcagaac
    10441 atcagctgga tcgagattgt cggcagccag ttcgagaaaa atatcgtagg cctgctcaag
    10501 cgtttcatct tcagtcaggc gattgtttag atccatatcc atagtcacta cctgtttaac
    10561 ctctgttggc gacgttttac agcaacgggc tgaagaagta aaacagtcgc tcggcgacac
    10621 gttgccataa tggacgtttt agccataaac gggcatcgag cagacgtgaa cgcgaaatat
    10681 aatcgtcctg aacggcggca aggtctgcac caaaaccttt atcgtcgatt gccagggtaa
    10741 tctcgaaatt cagccacaga ctacgcatat caaggttaac ggtgccaacc agacttagtt
    10801 cgccatcgac cagcacgctc ttggtatgca gtaacccgcc ttcaaactga taaattttaa
    10861 ccccagcagc aagcagttcc gtaaagaatg cgcgactggc ccagccgacc agcatcgagt
    10921 cattttttcg cggaaggata atactgacat ccaccccgcg ctgcgccgcc gtgcaaatcg
    10981 catgaagtaa atcatcgctt ggcacaaagt agggcgtggt catgatcaaa tattcacgcg
    11041 ccgaataagc cgcagtcaat aatgcctggt gaatgagatc ttccggaaag ccggggccag
    11101 aagcaattgt gtgaatggtg tgaccgctgg cctgttcaaa cggcataata ttgacatctg
    11161 gtggtggcgg cagaatacgt tttccggttt caatctccca gtcgcaggaa taaataatcc
    11221 ccatcgcggt ggcgatgggg ccttccatac gcgccatcag atcaatccat tgccctacgc
    11281 ccgcatcttg tttgaagtag cgaggatcga ccatattcat gctgccggtg tacgcgatgt
    11341 aattatcgat catgatcatc ttgcgatgtt ggcgcaggtc catacggcgt aaaaacacac
    11401 gcatcagatt gacctttaag gcttcgacca cttcaatacc ggcattacgc attagctcgg
    11461 gccacgggct gcggaaaaaa gccacactcc cggcggagtc gagcatcaat cggcaatgaa
    11521 taccacgccg tgcagccgcc attaatgatt cagccacctg gtccgccatg ccgccgggct
    11581 gccagatata aaacaccatc tcaatattat ggcgcgcgag ctggatgtcg cggattaacg
    11641 cctgcatcac atcatctgac tcggtcatca gttgtagctg attccctttg accccagcga
    11701 tcccctgacg acgctcgcaa agcttgaata atggcgcagc gacactgcta ttttcttcgg
    11761 cgaagatatg cttacaggct ttaaggtcgt taagccattt tgcggtggaa ggccacatcg
    11821 ctctggcgcg ctcagcgcgg cgtttgccta aatggagctc gccaacggca agataggcaa
    11881 taattccgac taacggcaga atgtaaataa tcaacagcca ggccatcgcg gagggaactg
    11941 cgcgtcgttt cattagaatg cgtaaagtta cgcctgcaat gagcaaccag tatcccagaa
    12001 tggccaacca actcaccaac gtataaacgg ttgtcataga ttagaaatcc ttttgaaagc
    12061 gcattgttat gagtttacgc atatggattc atctggcaaa taaaaacgcg ggaaaagcac
    12121 tggtcagcgt cgggggcggg ggtataatga ccattctgtt attgcataga gtagttaaca
    12181 tgaagcggag tagaacggaa gtggggcgct ggcgcatgca gcgtcaggct agccgacgta
    12241 aatcgcgttg gcttgagggg caatcgcgcc gaaatatgcg tatccacagc atcaggaagt
    12301 gcattctaaa caaacagcgt aactcgttat tgtttgcgat ctacaatatc taaatgtact
    12361 gggcaccatt attgcggtgc ccaataagtt tactcttccg tctttaaact ctatttttgc
    12421 gccgattctt tactgtcagc tttcgtttct tgttcatcgt tatcgctaaa aataccatga
    12481 cctgagttca acaacattga aacgagcata tcattattaa tctcttcacc atttaaaact
    12541 cgcgccagaa tttcgctgcc aaacagttgc ggtgagagaa tgatatccgg atgtaccatc
    12601 ttaatcttat tcaggttttt gctatcactg acggcgagaa ctgttttgac atcactgctc
    12661 atatcttttg ccgagagtac aacaaacgcg ttatctgcat cgttatcact cagcgccaga
    12721 atggcccggc aacgatcgat tcccgctttc tttaatactg aactgtcatt actgtcaccg
    12781 gggataacat cagcgttatc gcctaagcgt tgctcaagtt gcttgatatc atcttcaggc
    12841 aagttgctga taaccgttac gttttgtccg cgttgattca gttgcaggat cgtattgatg
    12901 gcgagaatcg aatgtccgca aacaataaaa tgatctttac gatgcattgt atgattgttt
    12961 ccttttaaaa gtttgttgaa tcccccgcgg ataagcggac caaaaattga ggtcatagat
    13021 gtggcaaaaa cggtaatgcc ggaaataatg accgaaatag tgaacaatcg tgctgattca
    13081 gaaacaggga caatatctcc atagccgacg gttgacatgg tttctatcga aaaatagaac
    13141 gcggtcatca aactttctat tcgcggatta aaaccttcgc ttaaataaag cgcaccgtag
    13201 gttgagtaaa acagtaatgt cgtgaaacta ataaatgcaa aaattgtccc ggctgcggcg
    13261 ctactgtggg agaagtcttt gcgcagtatc agcaaaaaca ccagcgtaaa aatgcaaaat
    13321 ccaatactaa atttcagcca gggataaaaa tgcagggtgt aaattagcgc tatcaacaac
    13381 aaaatgatac tgattgccca tgcgagcttt gcccggaaca gtaagccaat ggagttcagg
    13441 acaagaaaga cgccgagcat aaatagtggg gcgtttgcca gcaaggagag atccaactct
    13501 gaaaatgctt tgatatggaa aatatcaagc aaattgaccg acataccata gattgtttta
    13561 aaaataagca atccatttaa aaagacggcc agcgcgagaa tgtcgtgccg tagggtcacc
    13621 cataaatttg ttgctgtttg tttgaatgta gcccagtgac tcactgatac ctctgtaacc
    13681 acaattttgt ttaattatct tagcgtgcac attttctgaa catacatgca gcgcgtgaat
    13741 gtgttaaaca atagtagatg actttatccg cagtaatcaa aggttttgag tgagcaactc
    13801 tgcgtcagga aagggctttt ttcctgaaca tgctcacatc atataaagaa atacagataa
    13861 agttattatc tgcttgtggt agtgaatgca ctgaccggct ataaggaaag gccaaacaag
    13921 aacacggttg caaaaaccgt gcccttaaat attggatctc tactcagaac actttcttaa
    13981 atggtttcac tgaaacgtgt tcatagactc ctgccgctac gtacgggtca gcatcggccc
    14041 aggcctgagc tgcttccagc gattcaaatt cagcaataac ggttgagcca gtaaatcccg
    14101 cagcccctgg atcgttactg tctaccgctg gcattggacc agctgtcaac aaacgacctt
    14161 catcatgcag taactgtaaa cgtgctaaat gtgccggacg aacggaaagg cgtttttcga
    14221 ggctatcagc tttgtcttga gcgtaaataa catacaacac gggcacaact ccttgttcgg
    14281 gaaagttgta aagtacgtta tttgaaaggg cgaagatctg caacggaaag atgatgtctt
    14341 tgttaaggtc atgcataaag taagggtaat tacgccaaaa atgacatttt cactgatcct
    14401 gatcgtcttg ccttattgaa tatgattgct atttgcattt aaaatcgagg cttggttttt
    14461 ctactgaaat gattatgact tcaataaccc ttgatttacc tcgccgcttc ccctggccga
    14521 cgttactttc ggtctgcatt catggtgctg ttgtggcggg tctgctctat acctcggtac
    14581 atcaggttat tgaactacct gcgcctgcgc aaccaatttc tgtcacgatg gttgcgcctg
    14641 ctgatctcga accgccacaa gccgttcagc cgccaccgga gccggtggta gagccagaac
    14701 cggaacctga gccgatcccc gaaccgccaa aagaagcacc ggtggtcatt gaaaagccga
    14761 agccgaaacc taagccaaaa ccgaagccgg tgaaaaaggt acaggagcag ccaaaacgcg
    14821 atgtcaaacc cgtagagtcg cgtccggcat caccgtttga aaatacggca cccgcgcgcc
    14881 ccacatcaag tacggcaacg gctgcaacca gcaagccggt taccagtgtg gcttcaggac
    14941 cacgcgcatt aagccgtaat cagccgcagt atccggcacg agcacaggca ttgcgcattg
    15001 aagggcaggt taaagttaaa tttgacgtca cgccggatgg tcgcgtggat aacgtacaaa
    15061 tcctctcagc caagcctgcg aacatgtttg agcgtgaggt gaaaaatgcg atgcgcagat
    15121 ggcgttatga gccgggtaag ccaggcagtg ggattgtggt gaatatcctg tttaaaatta
    15181 acggcaccac cgaaattcag taagcagaaa gtcaaaagcc tccgaccgga ggcttttgac
    15241 tattactcaa caggtaaggc gcgaggtttt ccttcaggat caaccgcgac atacttaaat
    15301 aatgcttctg tcgctttata gcgttgcccg attggttcag acgctacttt tttcacccac
    15361 acttcaatat taatgctgac cgatgtcgtc cctttctgga cacagcgtgc atagcagcac
    15421 accacatcgc cgaccgcaac cggccgtaag aaagtcattc cttcaacccg cacagtcact
    15481 acgcgaccgt gggcaatttc ttttgccaga atagcgccgc caatatccat ttgtgacatt
    15541 aaccaaccac caaagatgtc accattggca ttggtatcgg cgggcatggc taaagtacgt
    15601 aaaacaagat cgccctgagg gacgttatgt gttgtagaca tggtaaaacc gacttaaaag
    15661 gaaaatcaca ggcgcgatgc tactatgatt ttcctgagga gaacagagga gatacagcgc
    15721 cagccccgaa ggactggcag tctggttagg atttatcttc ctgcggcatg tggcggtaga
    15781 tatagatacc gcttaacaat gtgaagatta aggtaagggc ggtcaggcca aagactttaa
    15841 agttgaccca aatattttgc ggcagccaga atgcgatgta gatgtttgcc agaccgcaaa
    15901 ggataaagaa aacagcccag gccagattta gcttcgacca taccggttgc ggcagcgtga
    15961 gttctttacc cagcatccgc tgaattagcg gctttttcat cacccattgg ctgactaaca
    16021 gggctcccgc aaacagagca taaatgactg taaccttcca tttaataaac tcatcattgt
    16081 ggaagaacag cgtcaagcca ccgaagacga ccaccagaac aaaagtgatc agggccatct
    16141 tctcaacctt acgaaagcga acccagctat agataagcac aatcgccgtg gcgacgatca
    16201 gcgctgcagt agccgcatag atgtcataaa tcttgtaaaa cgcgaaaaag acaaccagcg
    16261 gtaaaaaatc aagaaactgc ttcattttac gattccgtaa tcaaatgctt aaggttattg
    16321 gcgaatcaac atatacaggc ggaacagata gatgagcaat atggcagaaa tcaggttgct
    16381 caaggtgttc gccagtacgg caccaatttc cggggttaat gcggcaaaag aagaggcaaa
    16441 aagcagcaac agtgtttttg ccagcaacca gctcagtact gcgggtgcca ccagacgcat
    16501 attcgcccaa gtcagccgca tactgctacg catcgaggca aaaatgccca ttttgtcctg
    16561 taccagcatc accggtgcca gggccagtaa aatggctatg ataattcccg gaacgaccac
    16621 cagcatgatg ccaatctgta ctaaaagggt agtcagaaaa atcagaataa ataacttggg
    16681 caatatcggc gcactggcac caatagctcg cagcgcactg actctctgac ccgcagacac
    16741 cagctggata attaatatta cgcctccggc gagaatggcg ttaccgatta attctgaaaa
    16801 agtggacgcc gctgaagcct gcagcaaaat ttgttgctgt tccggtgaca tattctgaac
    16861 caggtcgaac aacccactac tgccgctaac gggcacgccg tcattgagct gcgcaagctg
    16921 tgcatcactg ggtgagaaaa catgccctaa caccactgtg ataaacgcac atagcaacga
    16981 taccaacaga atggtcataa attgattacg gaagaaattt ccggtgtcac ggtatacgga
    17041 ctgcgccgtg atagacatgc actctccttg agttttatgc aggtgttaat tagcgggcaa
    17101 ttgtaccctg gttaagcctc gggtggcagc atcaggtctt gtatggaaaa gcatatcttt
    17161 gtaaagcgga ggtaattgct ggccttcagg cgtgggcaaa ccgcggcaag tagcgatcga
    17221 gttaatattt gcgtagagaa aatatttaaa aattgattta aatcacatta accaggattc
    17281 tcaatgcaac ttctaaatta atccagatca ataaatggtg aattatcata tgtaatgtga
    17341 tctatgtagg atcatttgtt actccaatgt aggtatattc gtcacgtttt tataaccata
    17401 acgacggagc ggatatgaaa aagttaacag tggcggcttt ggcagtaaca actcttcttt
    17461 ctggcagtgc ctttgcgcat gaagcaggcg aattttttat gcgtgcaggt tctgcaaccg
    17521 tacgtccaac agaaggtgct ggtggtacgt taggaagtct gggtggattc agcgtgacca
    17581 ataacactca actgggcttg acgtttactt atatggcgac cgacaacatt ggtgtggaat
    17641 tactggcagc gacgccgttc cgccataaaa tcggcacccg ggcgaccggc gatattgcaa
    17701 ccgttcacca tctgccgcca acactgatgg cgcagtggta ttttggtgat gccagcagca
    17761 aattccgtcc ttacgttggg gccggtatta actacaccac cttctttgat aatggattta
    17821 acgatcatgg caaagaggcg gggctttccg atctcagtct gaaagattcc tggggagctg
    17881 ccgggcaggt gggggttgat tatctgatta accgtgactg gttggttaac atgtcagtgt
    17941 ggtacatgga tatcgatacc accgccaatt ataagctagg cggtgcacag caacacgata
    18001 gcgtacgcct cgatccgtgg gtgtttatgt tctcagcagg atatcgtttt taattccgca
    18061 caaaaacgac cccgtaatat acggggtcaa taaggacatg gtataaaggg tattatttct
    18121 tcgcttctac gccatcagtt tcagaacgaa ttaaaaattt ctcagttgtt tgcggaatat
    18181 gctgaatcag ccagtcggcc atttgctttt cctcatttaa aatcgcttcg atgatcggaa
    18241 ttgagactgt atcaccggca ttttttgctg ctgctaatag tgaggtgtaa caggcgattt
    18301 caaattgctc gaagacatat ccgctaatag agcctttaac tatttcatca gaagggaata
    18361 taccaccgat tgactgccca agcgcagcca ttttactcat ggaatcttta atgactgaac
    18421 gtgaaatgtc attacgatca agaatagttt ccagttgaac aatctggttt ttggtttcac
    18481 taagatgttg ttcaatacga gcgcgtagtt caggataatt atctatacgg ctggccatgg
    18541 attcaagcat agattcggct tgcttttcca ttgcgtgggc gtcacgtaac cagtcatgat
    18601 aatgttcaat acgattcatg aaaatgtcct catttataaa atgactaaaa gttaaaattc
    18661 atatttcagg ctttattttc ggctttctta tttacgttat taagggccag atcagtcagt
    18721 ttgatgtcgg tggccttttc ttcttccagg gtttctttca gaagcttcgc tgctttacgg
    18781 taacctaatt gttcagccag cgtcgctaat gtcccgtaac tggcaatctc ataatgctcg
    18841 actttctgtg ctgcggcaat cagtgcggca tcacgcactt cgtttttctc ggtactttcg
    18901 atgacctcat tagcttcttc aataagacct tccattgcca cacatttcat gcgcttaatt
    18961 ttcagatttg attctgattc cacaacttga tcaattcgtt caatctgtcc atgagtttcc
    19021 tcgaggtgcg catgaaaagc ctgacttaat ttttcatttg atgttgctct tgcgagtttt
    19081 gccagtgccc gggttaattg tttttctgcg ctgtaggtat ctgaaagcag ttgaataaat
    19141 acatcttcaa tggtcttcat attcatattt ttctccagtg aaatcactgc gagccatacg
    19201 gcccgcagat gcaattatct ttatcagctg atattaataa ttaacggtga atcaggattt
    19261 gcctgattta ttaccaccgc tttgttgacc gcctttttta cccgcttcag atgcgcgttg
    19321 cggatcattt ttaaaattac cgccgctatg ctgaccgcct ttacggcctg cgtcggatgc
    19381 cttctcacgg tcttcggcga aatttcctga accaccacga tgttcggcca tgttatttct
    19441 cccgttgcgt tgcattgttt cattaatatg agtgttgtgt gtcgacactc attaaaatta
    19501 gtcgataatg agaattagtc aaattaagcg caacgagaag atagagggaa aatatatttt
    19561 gaggaacatt ctggatatat taacaattac ctgaggaata agtgacttag agaaaattat
    19621 tgattttact ggtgttatgt tgcgggatta atttgtttga ttatattact gttgggcgga
    19681 aaaatgacgt aagttgacgt tcgaccaggg taagcgaaac ggtaaaaaga taaatattaa
    19741 atgaatttag gatttttccg gcttcattaa agaaagttaa aatgccgcca gcggaactgg
    19801 cggctgtggg attaactgcg cgtcgccgct ttcatcggtt gtacaaaaac tttcagtgcc
    19861 gccagcattt tctctggctc attaatatgt tgctcgatga ttttaacaat ggccgaacca
    19921 gaaatcgcgc ccgcagctcc tgcatcaatc gctgctttta cctgatccgg ggcgaaaata
    19981 ccaaacccct gcaatggagg tgcagcgttg tactctttca gcttcgcaac cagatgattg
    20041 aggggtaacg cggcgcggtt ttctgcgccg gtcacgcccg ctcgtgacag caaatatgtg
    20101 taaccacgac cgtaagaggc tatctggcgc agcaggtcgt catcggcatt tggcgggcag
    20161 atgaagatag gtgcgacatt atggcgcaac gcggcctggc ggaagggcgc ggactcttca
    20221 actggcacat cggcaaccag caccgaatcg acgccgactt tctcgcactg ggcataaaac
    20281 tcatcaatgc ctttgttaaa caccagattg gcatacatca acaggccaat gggaatggtc
    20341 gggtgtttct ggcgaatcag tgccagcatt tcaaaacatt gtgccggagt cacacctgcc
    20401 gcaaaggcgc gcagagtggc gttttgaatg gtcgggccat ccgccagtgg gtcggagaag
    20461 gggataccta actccagcgc gtcagcaccg gcttcaatta gcgtatcgat aattttcaat
    20521 gactgctcaa tgcccggatc accgagcgtg acgaaaggaa cgaatgcgcc ttctttgcgc
    20581 tccttcaact gggcaaacag agattcgtag cgttccatca gatttcccct cgtgctttca
    20641 aaatatcgtg aacggtgaag atgtctttat cgccgcgacc ggaaaggtta accaccagta
    20701 gctgctcttt atccgggttt tcgcgcatca ttttcagcgc atgggccagg gcgtgggagg
    20761 attccagcgc cgggatgatc ccttcgtgca ggcacagcgt tttgaaggct tcaagggctt
    20821 catcatcggt aatagacacg taatcagcgc gtccagtgct gttaagatac gcgtgttgtg
    20881 ggccgacaga cgggaaatcc agtccggcgg agatggagta agattcttca atctgcccgt
    20941 cttcggtttg catcatcggc gctttcatac cgaaataaat gcccacgcga ccatgtttta
    21001 gcggtgcgcc gtgctcgcca gtttcgatac cgtgaccact aggctccaca ccaatcaggc
    21061 cgacgttggt ttcattgatg aaatcagcaa acatgccgat ggcattcgaa ccgccgccaa
    21121 cacaggcgat aacggcatcc ggcaggcgac cttctctttc cagaatctgc gctttggttt
    21181 cttcgccaat catccgctga aactcacgga caatggtcgg ataaggatgc gggccagccg
    21241 cggtgcccag catatagtgc gcggtttcat aactgccgga ccagtcgcgt agcgcctcat
    21301 tacaggcatc tttcagcgtg gcggaaccgc tatgtaccgg gatcacttcc gcacccatta
    21361 agcgcatacg aaaaacgtta ggcgactggc gttcaacgtc tttggcaccc atataaatac
    21421 ggcatttcag gccgagcagg gcactggcaa gggccgacgc cacgccatgc tgaccggccc
    21481 cggtttcggc gatgatttcg gttttaccca tccgcttcgc cagcaacgcc tgccccagca
    21541 cctggttggt tttatgtgcg ccgccgtgca gcaaatcttc acgcttgaga tacagcgtgg
    21601 tgttcgtccc ggctgtaatg ttctggcatt tggtcagtgc ggttggacgc ccggcatagt
    21661 ttttcagcag gtcgttgaac tgggcttgaa attcaggatc tttttgcgcg ctgacaaaag
    21721 cttcttccag ctggcgcaga gcaggcatca ggatttgtgg cacgtacatg ccgccaaact
    21781 caccaaaata ggggttaagt aatgttgtca ttgttccttt ccttaatatg cgcgcagcgt
    21841 ctggaaaacc gaggccaaaa gacgtgcgtc tttgatgccc ggttgcgact ctacagcaga
    21901 attaaaatca agtccggcgc agccggtttg tgccgcttcc acgcagttat ctgcgcctaa
    21961 gccccccgcc agcagaacgt tgccaagcga ttgaccattt aatagtgacc agtcaaaacg
    22021 ttgcccgctt ccaccctggc cgttgtctaa aacatattta tcaacgtgct gaaactcgcg
    22081 ggcgggcagg gtttcaccga cgcttaatgc tttccagatg gcaacatgtg ctggcagagc
    22141 ttcacgcagc gtatcgatat acagctgatc ttcattacca tgcagttgca ctgccgccag
    22201 cgataacacc ttagctttgt ccaccacatc ggcaatatcg tgattgcgga acacgccaac
    22261 atactgcaac ggtgccgcag ccatcacttc ctgcgcctgt tcaacgttga cgcaacgcgg
    22321 tgatgtcgca acaaaaatca acccaccgta aatcgcgccc gcgtcataag ctgctttagc
    22381 atcttgccca cgcgtcaggc cacatacttt attctcaccc agcaacaccc ggcgcacggc
    22441 ggcgtgcaaa tcgtcatggg ccatcaacgc cgaaccaatc agaaaaccgt tagcgaagtg
    22501 gcttaactcg cgcacctgag cgtaagtatt gatgccggat tcgctgatta ccgttacgtt
    22561 gtgccccagt ttcggcgcaa gctcgcgggt acggttgaga tcaatcgaca aatcacgcag
    22621 atcgcggttg ttgatgccaa cgacctttgc ccctaatgca atggcgcgct ccagttcctc
    22681 ttcattactg acttcggtca gcacccccat ctccagactg tgagcgacgg ctgcaagctg
    22741 gcgatattgg tcgtcatcca gtactgaaag cattaataag caggcatcgg cctggtaata
    22801 gcgcgccaga tagatctggt aagggtcgat aatgaagtct ttacataaaa tcggctgcgg
    22861 ggcgatttgg ctgacgatgg ggaggaaatc aaagctcccc tgaaaatatt tctcatcagt
    22921 cagcaccgaa attgccgaag cgtaatgttt ataaatggcg gtaatgcgtg ccggatcgaa
    22981 atcatcacgg atcacgcctt ttgacggcga cgctttttta cactccagaa taaacgccgt
    23041 gcgtgcaccc tgtagcgcat cataaaaatg tcgtgtgctc ggctgaacct cattctgaaa
    23101 actggccagc ggttgctgct gtttgcgggt ttctacccaa atcgccttgt ctgcgacgat
    23161 tttcgctaaa acggtttgca tcatttaccc tcgtgccgcc agcgcggtaa ctctgtcgta
    23221 agcggaacca ctgcgcagta cctcaagaac ggtttgcgca ttggcttgca gatcttcatg
    23281 gccatgcagg cgcattaaca tggcgacgtt cgcagcgacg gctgcttcat gggcggcgtc
    23341 gcctttacct tgtaacaagc gtgttaaaat gtcacggttt tcttcaggtg ttccgcctgc
    23401 cagttgctcc tggtggtagg gagtcaggcc aaaatcttca gcggtcaatt gatagctctt
    23461 aattttgccg tcatgcagct cggcaacgat tgtcggcgcg tgtaatgaaa cttcatccat
    23521 cccgccgctg tgcaccaccg ccgcgcgttg ataccccagc acgcgcaagg tttcggcaat
    23581 cggcagcacc agttccggac tataaacacc aattaacgcc agcggcggat gcgccgggtt
    23641 aatcaatggc cccagcacat tgaacagggt gcgggttttc agttgctggc gaaccggcat
    23701 cgcgtggcgg aatccggtgt gatatttcgg cgcaaagagg aaacatacgc ctaactcgtc
    23761 cagcgcctgg cgcgatttat tggcgttcat atcaagatta ataccgaatg ccgccagcag
    23821 atccgacgag ccggatttac tggagacgct gcggttaccg tgtttcgcca ctttcagccc
    23881 acaggccgcg gcgacaaacg cactggcggt agaaatattg atactgttgc tgccgtcacc
    23941 gccagtaccg acgatatcag caaacagata atccgggcgc gggaacggcg ctgcgttttc
    24001 cagtagcgcg gttgctgccc cggcgatctc gttcgggtgc tcaccgcgaa ttttcatgct
    24061 caccagcgcc gccgccagtt gttccggctt cagctcgcca cgcaccaccg ctgaaaacag
    24121 ctggtggctt tcttgttggc taagcgtctg cgcctgatac agtttttcca gaatcggttg
    24181 cagcgtgttg gctggctcta gtttctgctg cgcccaggcc agcgtttgtt ccagcaggcg
    24241 agcgccctgg gtggtgagaa tggattccgg atggaactgg aatccacaaa cgcgatccgc
    24301 atcgtgacgt actgccatca ccatgccatt aaaatgggcg ttgatggtta aaccggccgg
    24361 aatgttactg ccaaccagcg agtgataacg cgccaccggc agcgggtttg ttaatccggc
    24421 aaacatcgcc tgaccgtcat gttcaatgct cgacgcttta ccgtgaagaa tttcgcccgc
    24481 ctgaccgaca tagcccccgt aagcttcgac aattgcctga tgtccgaggc aaatgccaat
    24541 aatgggcagc ttgccacgca agcgggtgag gagttccggc atacaaccgg cttcgctcgg
    24601 cacaccgggg ccaggagaaa gcatcagtac cggattgctc atcgtcgcca ggcgttcaat
    24661 taaggtctgc gccggaatat ggttgcggta aatcaccacg ttatgaccat tgctgcgcaa
    24721 ctgatctgcc aggttgtacg taaaagagtc gatattatcg agcagcagaa tgtcagccat
    24781 cagaaagtct cctgtgcatg atgcgcggtg gcaatagcgc gcagtacagc gcgggctttg
    24841 ttacgggttt cgtcggcttc cgactgcgga acagaatcaa ggactacacc agcacccgct
    24901 tgcacggtgg cgataccgtt ttccaccagc gccgagcgga tcacaatgca ggtgtcgaga
    24961 tcgccatgcg cggtgaaata acctaccgcg ccgccgtagc tgccgcggcg acgaccttcc
    25021 gcctcggcaa ttaactgcat agcgcgtact ttcggcgcac cgcttaacgt ccccatattc
    25081 atacaggcgc gataagcgtg cagggcgtca agatcgtgac gcagttcgcc gactacgcga
    25141 gagacgaggt gcatcacata ggaataacgg tcaactttgg tgagatcggc gacgtagcgg
    25201 ctgccagggg tgcaaatgcg tgccagatca ttacgggcga gatcaaccag catcagatgt
    25261 tcagacagct ctttatggtc agtacgcatt tccagttcaa tacggctgtc gagatctctg
    25321 tccagtgaac catcggcgcg acgaccgcgt gggcgtgttc cggcaatcgg gtagatctca
    25381 atctggcggc tggtggcatc atacttgagc gagctttccg gcgacgcgcc aaatagggtg
    25441 aaatcattat cctgcataaa aaacatgtac gggctgggat tactcttttt cagcacgtaa
    25501 taggccgcca gcggtgacgg gcagggcaga gagaaacggc gagatggcac cacctggaaa
    25561 atttctccag cgcgaatcgc tttttgcaac aaacgcacta cgccaccgaa ctcttcatcg
    25621 ctctgattac attcacaacg catatgcggc acggaaacca ctggcagcgg cggcgcggct
    25681 tcggtcagtt gctgacgtag ttcgttcagg cgagcagtga gacgttgttt ttcttcttca
    25741 ttcggagcaa acaggctggc ctgaatacgg gtgctttttt tctgatggtc aatcaccatc
    25801 agcgtttcag cgagataaaa acagaaatca gggcagttat tttccgctga cagttgcggt
    25861 aaatcttcaa atcccgccac aaggtcataa gagaacaggc cgccgaagaa cattgcttct
    25921 cgttcttcct tcggtacatt caacagattc tgcaataaac ggaaagcgtc aaaaaccgaa
    25981 agggagcata agcgggcgtc ttcatccagc agtggactga caggggggaa gcgcagcaca
    26041 cggcagtttg gtgattgttc actttccaca cccgcaggca gggcgttatc cagtagtgcc
    26101 agcagggctt cgccattgcc ggaaagtgcc tgaattgtga cggtgtcacc taatgctgta
    26161 atgcgcagcg cactgtctat cagcagcagg ctttttaaat catctttgct gtcgatatct
    26221 gcggattcca gcagcagcgt tgccggacga ttcccacaca actggtgaaa aagcgcagtc
    26281 gggttgtcgc gataagcgcc ttcgcaggtt agcagttcga gagtcggttt ttgtgtttgc
    26341 attgttattc tctaattttg ttcaaaaaaa agcccgctca ttaggcgggc tgggtatctg
    26401 attgctttac gcatggtgaa tacactgccc gtttcaggaa gtgcgccacc aacctttcag
    26461 tacgaaaatt gctttcattg tcgataccct ttttacgtga acttgcgtac tagttaactg
    26521 gttcgatgat taattgtcaa cagctcattt cagaatattt gccagaaccg ttatgatgtc
    26581 ggcgcaaaaa acattatcca gaacgggagt gcgccttgag cgacacgaat tatgcagtga
    26641 tttacgacct gcacagccat accacagctt ccgatggctg cctgacgcca gaagcattgg
    26701 tgcaccgtgc agtcgagatg cgcgtcggca ccctggcgat caccgaccat gacaccacag
    26761 ctgcaatcgc gccagcaaga gaggaaattt cacgttcggg actggcgctg aatcttattc
    26821 ccggcgtgga aatttccacg gtctgggaaa atcatgaaat tcatattgtc gggctgaata
    26881 ttgatattac tcatccgcta atgtgtgagt tcctcgcgca gcagacagaa cggcgcaatc
    26941 agcgggcgca gcaaattgcc gaaagacttg aaaaagcgca aatacctggg gcgctggaag
    27001 gcgcacaacg actggcgcag ggcggcgcag tgacgcgcgg tcattttgcg cgtttccttg
    27061 tggagtgcgg caaagccagt tcaatggcag atgtctttaa aaagtatctg gcgcgcggga
    27121 aaaccggata cgttccgcca cagtggtgta caatagaaca agctattgat gtcattcatc
    27181 attctggcgg taaggcggtg ctagctcatc ctgggcggta caatcttagt gctaaatggc
    27241 tgaaaagatt ggttgcgcat tttgccgaac accacggcga cgcgatggaa gtcgcgcagt
    27301 gccagcaatc gcccaatgaa cgcacccagc tggccacact tgcgcgtcag catcatttat
    27361 gggcatcaca aggatctgat tttcatcagc catgcccgtg gatcgaactg ggtcgtaaac
    27421 tctggctgcc cgcaggcgtt gaaggcgtct ggcagctatg ggaacagccg cagaacacca
    27481 cagagaggga attatgagcc agttttttta tattcatcct gataacccgc agcaacgtct
    27541 gatcaaccag gcggtggaga tcgtgcgtaa aggcggggtg attgtctatc caactgattc
    27601 cggctatgcg ctcggctgta aaattgaaga taaaaacgcc atggagcgta tttgtcgtat
    27661 tcgccagctg ccggacggtc acaactttac cctgatgtgt cgcgatcttt ctgaactctc
    27721 gacctattca ttcgtggata atgttgcgtt tcgtttgatg aaaaacaaca cgccgggcaa
    27781 ctataccttc atcctgaagg ggacgaagga agtgccacgc cgtctgttgc aggagaaacg
    27841 caaaaccatc ggtatgcgtg tgccttctaa cccgattgcg caggcgttgc ttgaagcact
    27901 gggcgaaccg atgctttcca cttcgctaat gctgccaggc agcgaattta ccgaatccga
    27961 tcccgaagaa attaaagacc gtctggaaaa acaggtagat ttgattattc acggcggcta
    28021 tctcgggcag aaaccaacaa cggttattga tcttaccgat gatacgccgg tcgtggtgcg
    28081 tgaaggcgta ggtgatgtga agcctttctt ataagtgcgt aactctgtat actacgcggc
    28141 cttaaaatgg cgctatacgg cgccattgat tctcctgttc gacgcctgtg aaggcgacac
    28201 caaaaggaag ctctatgagc gaaaaattac agaaagtgct ggcccgcgct ggccacggct
    28261 cccgccgtga aatcgaatct attattgaag ccggtcgtgt gagtgttgat ggcaaaattg
    28321 ctaaactcgg cgatcgcgtt gaagttaccc ctgggctgaa aattcgtatc gatggtcatc
    28381 tgatttcggt acgtgagtcc gctgaacaaa tttgtcgcgt tctggcctat tacaaaccgg
    28441 aaggtgagtt gtgtacccgt aacgatccgg aagggcgtcc gacggtgttt gaccgtctgc
    28501 caaagctgcg tggcgcacgc tggattgccg taggtcgtct ggacgttaac acctgtggtc
    28561 tgctgctgtt caccaccgat ggtgaactgg caaaccgttt aatgcatcca agccgtgaag
    28621 ttgaacgtga atacgccgtg cgtgtatttg gtcaggttga cgacgcgaaa ctgcgtgatt
    28681 tgagtcgtgg cgtgcaactg gaagatggtc cggcagcttt taaaaccatc aagttcagcg
    28741 gcggcgaagg gatcaaccag tggtacaacg tgactctgac cgaaggccgt aaccgtgaag
    28801 ttcgtcgtct gtgggaagcg gttggtgtgc aggtgagccg cctgatccgt gttcgttacg
    28861 gtgatatccc actgccaaaa ggtctgccac gcggtggctg gacggagctg gacctcgccc
    28921 agactaacta tctgcgcgaa ctggtggagc ttccgccaga aaccagctct aaagtcgctg
    28981 tagaaaaaga ccgtcgtcgc atgaaggcta atcagattcg tcgtgcggtg aaacgtcaca
    29041 gtcaggtgag cggcggtcgt cgttctggcg ggcgtaataa cggttaatca aagtatgccg
    29101 gacgtcatat ccggattaat aatcaatccc tatttgcgcc ttaacaccgg catcaaacgc
    29161 atgtctgatg gggcgcagct cacttaccgt atccgccagt tcaagaatat cccgatgaca
    29221 gcctcgaccc gtgataatca ccgtctgttg atgtggacgt tcatttaacg cctgcaccac
    29281 ttcttccagt ggcaaatagt catacgccac catatacgtc agttcatcaa gcaaaaccat
    29341 atccagtgag gaatcagcaa gcatccgctt tgcatgttgc cagactttgc ggcaggcggc
    29401 ggtatcagac tcgcggtttt gtgtatccca ggtaaagccc gttgccatca cctgaaactc
    29461 aacgccatgt ggctccagca gattgcgttc gccattaggc caggtgcctt taataaactg
    29521 cacgacgccc actttttttc cgtgaccaac tgcgcgtgtt gccgtaccaa atgccgcggt
    29581 ggttttgcct tttccattgc cggtaaagac gatgataata ccgcgttcat cctgggcctg
    29641 ggccacacgg gcatctactt tttctttcac tcgctgctga cgctgttggt agcgttcatc
    29701 actcattggg aaattcctgg tttacggccc ggttgggcgt caaaggtcat gccggtttta
    29761 cggcggctgt catcgcccat cagccagagg tagagcggca tgatatcagc gggtgtttta
    29821 agtttctgtg gatcttcggt cgggaaggcg cttgcgcgca ttgcggtgcg cgtaccgcct
    29881 gggttaatgc agttgacgcg caggcgctgc tgatattcat cggccagtac ctgcatcatc
    29941 ccttcggtgg caaatttcga cgctgcatat gcaccccagt tggctcgtcc ctgacgtcca
    30001 acgcttgatg aagtaaagac cagtgaaccg gcgtccgatt tgagtaataa aggaagcagt
    30061 gcctgggtga gcataaaggt ggcattaacg ttgacctgca tgacgtcctg ccagacctgc
    30121 ggattttgtt cgctcattgg gcaaacatcg ccgagcaatc cggcattatg caaaacacca
    30181 tccagacgcg gataattaac gacaatgcgc tgtgccagtt gttggcaatt ttcggacgtg
    30241 caggtcagca aatcgagaat aaaccactgt ggctgacgcc cagtttcttc gtttatgtgg
    30301 ctggctacct gacgtaattt ttcttcatta cggcccaaca gaatcactgt cgcaccatag
    30361 cgtgcatacg tcatcgcggc ttcacgacca atcccatcgc tggctcccgt caccaggata
    30421 atgcgatcat tgagtaaatc ttgttttggc tggtaatgca tggctactcc tcaacgacgt
    30481 tgtctgtcgc gctgccagtg tactttatga ctttggggct ttatgcctga aacagaaggg
    30541 catttcaatc agcctggggc gataacaccg caaaatggat actttgtcat actttcgctg
    30601 caataacatc tctgcgagac ggcttaacat gcctgttgta aactgtgagc caaagcgttg
    30661 tttaaccaag gtggggactc gtggaattgt tgtctgaata tggtttgttt ttggcgaaaa
    30721 tcgttaccgt tgtgctagcg attgcggcga ttgccgccat tattgtcaat gttgctcaac
    30781 gtaataaacg ccagcgtggc gagttacggg tcaacaatct cagcgaacag tataaggaga
    30841 tgaaagaaga actggccgcg gcgctgatgg actcacatca gcaaaaacag tggcacaaag
    30901 cgcagaagaa aaagcacaag caagaagcga aagcagcaaa agcgaaagcc aaactgggcg
    30961 aggtggcaac tgacagtaaa cctcgcgtct gggtgctgga ttttaaaggc agcatggacg
    31021 cccatgaagt gaactcgcta cgtgaagaga taacggcggt actcgcagca ttcaaaccgc
    31081 aggatcaggt tgtgctccgt ctggaaagcc caggtggcat ggtgcatggt tacgggttgg
    31141 cggcttcgca gctgcagcgt ctgcgtgata aaaacattcc tttaactgtt acggtagaca
    31201 aagtcgctgc cagcggcggt tacatgatgg cctgtgtggc ggacaaaatt gtttccgcac
    31261 cgtttgctat tgtgggttcc attggggtgg tggcgcaaat gcccaacttt aaccgcttcc
    31321 tgaaaagcaa agatattgat atcgaactgc acaccgccgg gcagtataag cgcacgctga
    31381 ccttgctggg tgaaaatacc gaagaagggc gggagaaatt ccgcgaagag ctgaacgaaa
    31441 cgcatcagtt atttaaagat tttgtgaagc gtatgcgtcc gtctctggat attgaacagg
    31501 tggcaacggg tgaacactgg tacggacaac aggcggtaga gaaaggcctg gttgatgaaa
    31561 tcaacaccag tgatgaagtt attcttagcc tgatggaagg ccgtgaagtg gttaatgtac
    31621 gctatatgca gcgtaaacga ctcattgacc gattcaccgg cagcgcggca gagagcgccg
    31681 atcgattgtt gctacgctgg tggcagcggg gtcaaaagcc attgatgtaa aagacaaacg
    31741 cgaggctaag acctcgcgtt ttgctttaat caaccagatg atatttttct gaaagcacat
    31801 gggccaggtg tttgaacata ttaaacaccg cggtgctttt ggctgttggc aatccttgtt
    31861 catctaaaaa gtagtcgccg gtaaatacca gcacgccatt acgctgcgtg acgccggtgg
    31921 cttcaatccc tgcgagcgta tcctcatgct cacgaatgat tttgttggcc tctttcaaca
    31981 gcgtttcgcg gtcgatgggt tgtgtctctt tgttcattat ttactcctta aacaaggaca
    32041 ttagtctacg ccaggcatgg cttgcagaca aatataccac gctggtggca agagcgcctt
    32101 actggcaact ttggattttg catgctaata aagttgcgta tcggatttta tcaggtacag
    32161 tgtgacgctt tcgtcaatct ggcaatagat ttgcttgaca ttcgaccaaa attccgtcgt
    32221 gctatagcgc ctgtaggcca agacctgtta actcagtcac ctgaattttc gtgaacagag
    32281 tcacgacaag gggttgatat ccgcagagag cgagtccata tcggtaactc gttgccagtg
    32341 gaaggtttat caacgtgcga cgcattcctg gaagaatcaa attaggtaag gtgaatatgg
    32401 gtaaagctct tgtcatcgtt gagtccccgg caaaagccaa aacgatcaac aagtatctgg
    32461 gtagtgacta cgtggtgaaa tccagcgtcg gtcacatccg cgatttgccg accagtggct
    32521 cagctgccaa aaagagtgcc gactctacct ccaccaagac ggctaaaaag cctaaaaagg
    32581 atgaacgtgg cgctctcgtc aaccgtatgg gggttgaccc gtggcacaat tgggaggcgc
    32641 actatgaagt gttgcctggt aaagagaagg tcgtctctga actgaaacaa ctggctgaaa
    32701 aagccgacca catctatctc gcaaccgacc ttgaccgcga aggggaagcc attgcatggc
    32761 acctgcggga agtgattggt ggtgatgatg cgcgctatag ccgagtggtg tttaacgaaa
    32821 ttactaaaaa cgcgatccgc caggcattta acaaaccggg tgagctgaat attgatcgtg
    32881 ttaatgccca gcaggcgcgt cgctttatgg accgcgtggt ggggtatatg gtttcgccgc
    32941 tgctgtggaa aaagatcgct cgtggtctgt ctgccggtcg tgtgcagtcg gtggcggtcc
    33001 gcctggtggt agagcgtgag cgtgaaatta aagcgttcgt accggaagag ttctgggaag
    33061 tcgatgccag cacgaccacg ccatctggtg aagcgttggc gttgcaggtg actcatcaga
    33121 atgacaaacc gttccgtccg gtcaacaaag aacaaactca ggctgcggta agtctgctgg
    33181 aaaaagcgcg ctacagcgtg ctggaacgtg aagacaaacc gacaaccagt aaacctggcg
    33241 ctccttttat tacctctacg ctgcaacaag ctgccagcac ccgtcttggt tttggcgtga
    33301 aaaaaaccat gatgatggcg cagcgtttgt atgaagcagg ctatatcact tacatgcgta
    33361 ccgactccac taacctgagt caggacgcgg taaatatggt tcgcggttat atcagcgata
    33421 attttggtaa gaaatatctg ccggaaagtc cgaatcagta cgccagcaaa gaaaactcac
    33481 aggaagcgca cgaagcgatt cgtccttctg acgtcaatgt gatggcggaa tcgctgaagg
    33541 atatggaagc agatgcgcag aaactgtacc agttaatctg gcgtcagttc gttgcctgcc
    33601 agatgacccc agcgaaatat gactccacga cgctgaccgt tggtgcgggc gatttccgcc
    33661 tgaaagcacg cggtcgtatt ttgcgctttg atggctggac aaaagtgatg cctgcactgc
    33721 gtaaaggcga tgaagatcgt atcttaccgg cagtcgataa aggcgatgct ctgacgctcg
    33781 ttgaactaac accagcccag cactttacca agccgccagc ccgtttcagt gaagcatcgc
    33841 tggttaaaga actggaaaaa cgtggtatcg gtcgtccgtc tacctatgcg tcgatcattt
    33901 cgaccattca ggatcgtggc tatgtgcgag tagaaaatcg tcgtttctat gcggaaaaaa
    33961 tgggcgaaat cgtcaccgat cgccttgaag aaaatttccg cgagttaatg aactacgact
    34021 ttaccgcgca gatggaaaac agcctcgacc aggtggcaaa tcacgaagca gagtggaaag
    34081 ctgtactgga tcacttcttc tcggatttca cccagcagtt agataaagct gaaaaagatc
    34141 cggaagaggg tggtatgcgc ccgaaccaga tggttctgac cagcattgac tgcccgactt
    34201 gtggtcgcaa aatggggatt cgcacagcga gcaccggggt attccttggc tgttctggct
    34261 atgcgctgcc gccgaaagag cgttgcaaaa ccaccattaa cctggtgccg gaaaacgaag
    34321 tgctgaacgt gctggaaggc gaagatgctg aaaccaacgc gctgcgcgca aaacgtcgtt
    34381 gcccgaaatg cggcacggcg atggacagct atctcatcga tccgaaacgt aagttgcatg
    34441 tctgtggtaa taacccaacc tgcgacggtt acgagatcga agagggcgaa ttccgcatta
    34501 aaggttatga cggcccgatc gttgagtgtg aaaaatgtgg ttctgaaatg cacctgaaaa
    34561 tggggcgttt cggtaaatac atggcctgca ccaacgaaga gtgtaaaaac acacgtaaga
    34621 ttttacgtaa cggcgaagtg gcaccaccga aagaagatcc ggtgccatta cctgagctgc
    34681 cgtgcgaaaa atcagatgct tatttcgtgc tgcgtgacgg tgctgccggt gtgttcctgg
    34741 ctgccaacac tttcccgaaa tcgcgtgaaa cgcgtgcgcc actggtggaa gagctttatc
    34801 gcttccgcga ccgtctgccg gaaaaactgc gttatctggc cgatgcgcca cagcaggatc
    34861 cggaaggtaa taagaccatg gttcgcttta gccgtaaaac caaacagcaa tatgtctctt
    34921 cggaaaaaga cggaaaggcg actggctggt cagcatttta tgttgatggc aaatgggttg
    34981 aaggaaaaaa ataaccttta attctgtcag gtttttataa acaaagggtc gcgaaagcgg
    35041 ccctttttta ttgcatatta ttttttcttc acacctatac actaaggcta taaatgatat
    35101 agtggttata gttagcacct tttttattat taaatcgtat tagtcacccg ccaggtgtga
    35161 cgaaaaaacg atgttctgat ggcgtctaag tggatggttt aacatgaaat tacaacaact
    35221 tcgctatatt gttgaggtgg tcaatcataa cctgaatgtc tcatcaacag cggaaggact
    35281 ttacacatca caacccggga tcagtaaaca agtcagaatg ctggaagacg agctaggcat
    35341 tcaaattttt tcccgaagcg gcaagcacct gacgcaggta acgccagcag gacaagaaat
    35401 aattcgtatc gctcgcgaag tcctgtcgaa agtcgatgcc ataaaatcgg ttgccggtga
    35461 gcacacctgg ccggataaag gctcgctgta tatcgccacc acgcataccc aggcacgcta
    35521 cgcgttacca aacgttatca aaggctttat tgagcgttat ccccgcgttt ctttgcatat
    35581 gcaccagggc tcgccgacac aaattgctga tgccgtctct aaaggcaatg ccgatttcgc
    35641 tatcgccaca gaagcgctgc atctgtatga agatttagtg atgttaccgt gctaccactg
    35701 gaatcgggct attgtagtca ctccggatca cccgctggca ggcaaaaaag ccattaccat
    35761 tgaagaactg gcgcaatatc cgttggtgac atataccttc ggctttaccg gacgttcaga
    35821 actggatact gcctttaacc gcgcagggtt aacgccgcgt atcgttttca cggcaacgga
    35881 tgctgacgtc attaaaactt acgtccggtt agggctgggg gtaggggtca ttgccagcat
    35941 ggcggtggat ccggtcgccg atcccgacct tgtgcgcgtt gatgctcacg atatcttcag
    36001 ccacagtaca accaaaattg gttttcgccg tagtactttt ttgcgcagtt atatgtatga
    36061 tttcattcag cgttttgcac cgcatttaac gcgtgatgtc gttgatgcgg ctgccgcatt
    36121 gcgctccaat gaagaaattg aggtcatgtt taaagatata aaactg
//
LOCUS       DBKEKI010000028        35940 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.028, whole genome shotgun sequence.
ACCESSION   DBKEKI010000028 DBKEKI010000000
VERSION     DBKEKI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.028"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..798)
                     /locus_tag="ACUQPX_001360"
     CDS             complement(<1..798)
                     /locus_tag="ACUQPX_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHV9056119.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKL"
     gene            complement(812..1471)
                     /gene="pgmB"
                     /locus_tag="ACUQPX_001361"
     CDS             complement(812..1471)
                     /gene="pgmB"
                     /locus_tag="ACUQPX_001361"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HHV9056120.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNTPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1468..3735)
                     /gene="ycjT"
                     /locus_tag="ACUQPX_001362"
     CDS             complement(1468..3735)
                     /gene="ycjT"
                     /locus_tag="ACUQPX_001362"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HHV9056121.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGYVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(3732..4787)
                     /locus_tag="ACUQPX_001363"
     CDS             complement(3732..4787)
                     /locus_tag="ACUQPX_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HHV9056122.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAWEMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQLIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4797..5585)
                     /locus_tag="ACUQPX_001364"
     CDS             complement(4797..5585)
                     /locus_tag="ACUQPX_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HHV9056123.1"
                     /translation="MKIGTQNQAFFPENIRERFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5603..6655)
                     /locus_tag="ACUQPX_001365"
     CDS             complement(5603..6655)
                     /locus_tag="ACUQPX_001365"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HHV9056124.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6686..7528)
                     /locus_tag="ACUQPX_001366"
     CDS             complement(6686..7528)
                     /locus_tag="ACUQPX_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHV9056125.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7515..8396)
                     /locus_tag="ACUQPX_001367"
     CDS             complement(7515..8396)
                     /locus_tag="ACUQPX_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHV9056126.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8417..9700)
                     /locus_tag="ACUQPX_001368"
     CDS             complement(8417..9700)
                     /locus_tag="ACUQPX_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHV9056127.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNRQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLASLTITAGQKTEAAEKFVTFMEQADNIADWVMMSPG
                     AALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDVTGS
                     GVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9714..11391)
                     /gene="ycjM"
                     /locus_tag="ACUQPX_001369"
                     /pseudo
     CDS             complement(9714..11391)
                     /gene="ycjM"
                     /locus_tag="ACUQPX_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(11603..11950)
                     /gene="pspE"
                     /locus_tag="ACUQPX_001370"
     CDS             complement(11603..11950)
                     /gene="pspE"
                     /locus_tag="ACUQPX_001370"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HHV9056128.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(11993..12214)
                     /gene="pspD"
                     /locus_tag="ACUQPX_001371"
     CDS             complement(11993..12214)
                     /gene="pspD"
                     /locus_tag="ACUQPX_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HHV9056129.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12223..12582)
                     /gene="pspC"
                     /locus_tag="ACUQPX_001372"
     CDS             complement(12223..12582)
                     /gene="pspC"
                     /locus_tag="ACUQPX_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HHV9056130.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12582..12806)
                     /gene="pspB"
                     /locus_tag="ACUQPX_001373"
     CDS             complement(12582..12806)
                     /gene="pspB"
                     /locus_tag="ACUQPX_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HHV9056131.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12860..13528)
                     /gene="pspA"
                     /locus_tag="ACUQPX_001374"
     CDS             complement(12860..13528)
                     /gene="pspA"
                     /locus_tag="ACUQPX_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HHV9056132.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13695..14672
                     /gene="pspF"
                     /locus_tag="ACUQPX_001375"
     CDS             13695..14672
                     /gene="pspF"
                     /locus_tag="ACUQPX_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HHV9056133.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14792..16057)
                     /gene="puuE"
                     /locus_tag="ACUQPX_001376"
     CDS             complement(14792..16057)
                     /gene="puuE"
                     /locus_tag="ACUQPX_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HHV9056134.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(16095..17375)
                     /gene="puuB"
                     /locus_tag="ACUQPX_001377"
     CDS             complement(16095..17375)
                     /gene="puuB"
                     /locus_tag="ACUQPX_001377"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HHV9056135.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(17377..18864)
                     /gene="puuC"
                     /locus_tag="ACUQPX_001378"
     CDS             complement(17377..18864)
                     /gene="puuC"
                     /locus_tag="ACUQPX_001378"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HHV9056136.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFEHGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(19014..19571)
                     /gene="puuR"
                     /locus_tag="ACUQPX_001379"
     CDS             complement(19014..19571)
                     /gene="puuR"
                     /locus_tag="ACUQPX_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HHV9056137.1"
                     /translation="MSDEGLAPGKRLSEIRLQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19598..20350)
                     /gene="puuD"
                     /locus_tag="ACUQPX_001380"
     CDS             complement(19598..20350)
                     /gene="puuD"
                     /locus_tag="ACUQPX_001380"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HHV9056138.1"
                     /translation="MNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHA
                     LAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINAALER
                     RIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQVEEG
                     GLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWH
                     PEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            20574..21992
                     /gene="puuA"
                     /locus_tag="ACUQPX_001381"
     CDS             20574..21992
                     /gene="puuA"
                     /locus_tag="ACUQPX_001381"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HHV9056139.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGYYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHTCKN
                     DELLQFERLITETEIEWMLKNA"
     gene            22141..22305
                     /locus_tag="ACUQPX_001382"
     CDS             22141..22305
                     /locus_tag="ACUQPX_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056140.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            22295..23680
                     /gene="puuP"
                     /locus_tag="ACUQPX_001383"
     CDS             22295..23680
                     /gene="puuP"
                     /locus_tag="ACUQPX_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HHV9056141.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23814..24059
                     /locus_tag="ACUQPX_001384"
     CDS             23814..24059
                     /locus_tag="ACUQPX_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HHV9056142.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24372..26015
                     /gene="sapA"
                     /locus_tag="ACUQPX_001385"
     CDS             24372..26015
                     /gene="sapA"
                     /locus_tag="ACUQPX_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HHV9056143.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            26012..26977
                     /gene="sapB"
                     /locus_tag="ACUQPX_001386"
     CDS             26012..26977
                     /gene="sapB"
                     /locus_tag="ACUQPX_001386"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HHV9056144.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26964..27854
                     /gene="sapC"
                     /locus_tag="ACUQPX_001387"
     CDS             26964..27854
                     /gene="sapC"
                     /locus_tag="ACUQPX_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HHV9056145.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27854..28846
                     /gene="sapD"
                     /locus_tag="ACUQPX_001388"
     CDS             27854..28846
                     /gene="sapD"
                     /locus_tag="ACUQPX_001388"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HHV9056146.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28848..29654
                     /gene="sapF"
                     /locus_tag="ACUQPX_001389"
     CDS             28848..29654
                     /gene="sapF"
                     /locus_tag="ACUQPX_001389"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HHV9056147.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29722..30075
                     /locus_tag="ACUQPX_001390"
     CDS             29722..30075
                     /locus_tag="ACUQPX_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HHV9056148.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            30443..31231
                     /gene="fabI"
                     /locus_tag="ACUQPX_001391"
     CDS             30443..31231
                     /gene="fabI"
                     /locus_tag="ACUQPX_001391"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HHV9056149.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            31375..32502
                     /locus_tag="ACUQPX_001392"
     CDS             31375..32502
                     /locus_tag="ACUQPX_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HHV9056150.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            32570..34504
                     /gene="rnb"
                     /locus_tag="ACUQPX_001393"
     CDS             32570..34504
                     /gene="rnb"
                     /locus_tag="ACUQPX_001393"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HHV9056151.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCGQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(34582..34683)
                     /locus_tag="ACUQPX_001394"
     CDS             complement(34582..34683)
                     /locus_tag="ACUQPX_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056152.1"
                     /translation="MTYMKLTAAKRIVFIYLFVIQFWKNASFQTKVS"
     gene            34737..>35940
                     /locus_tag="ACUQPX_001395"
     CDS             34737..>35940
                     /locus_tag="ACUQPX_001395"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HHV9056153.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDALRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRTQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIKVYTSCSVGISLSPEHGSDSATIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQ"
BASE COUNT         8311 a         9566 c         9212 g         8851 t
ORIGIN      
        1 taatttctct tcgggaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
       61 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
      121 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
      181 cacaatccgc gtcgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
      241 ggtgttcagt ttctgatgca gcttgctgat ctctgcgcgc atttgtacgc gcagcttggc
      301 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
      361 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
      421 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
      481 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
      541 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
      601 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
      661 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
      721 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
      781 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
      841 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
      901 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
      961 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
     1021 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcgc
     1081 gcagctccag cgccgctaaa atcgtcggcg tattcaggga gacggaagcc agccctaccg
     1141 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
     1201 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
     1261 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
     1321 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
     1381 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
     1441 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
     1501 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
     1561 ctgaccgttc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
     1621 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
     1681 ctcaggtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
     1741 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
     1801 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
     1861 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
     1921 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
     1981 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
     2041 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
     2101 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
     2161 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
     2221 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
     2281 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
     2341 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
     2401 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
     2461 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
     2521 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
     2581 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
     2641 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
     2701 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtagc ctttgtagcc
     2761 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg tcgtgagccg gcgtcatgat
     2821 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
     2881 cgtgatacga cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
     2941 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
     3001 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
     3061 ctcgcctgca tgaagctgcg cactggtatg ttgcagtaaa cggcgctctt tggcggtata
     3121 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
     3181 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
     3241 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
     3301 tgacgcgtcg gcgtccagtg gcgtaatagt gatttccagc gcaatgagcg gcagttggtc
     3361 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
     3421 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
     3481 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
     3541 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
     3601 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
     3661 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
     3721 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
     3781 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
     3841 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
     3901 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
     3961 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
     4021 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
     4081 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
     4141 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
     4201 tacatcgcag catccagcat atgaatgccg atgtcgatca gcgggccacc accctgcagt
     4261 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
     4321 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
     4381 agcgcaaaac gatggtgaaa gtcgtaggcc agcaccttgc ccagtttgcg cgcggtatcg
     4441 cacatttccc acgcctgttc tggcgtcatg gcgggcggtt tttcgcacat cacatggcag
     4501 cctgcttcca gtgccatcag ggtatgttcg taatgaaaac ggttaggtga gcagacgcta
     4561 accacatcag gtttcaccgc cagcagcatg gcctgcggat cgtcccacac ggatgcattc
     4621 ccgtattttt ctgccagcgc ctgcgcctgg gaaaggcggc tgtcacagac agccaccagt
     4681 tccagatcgt tgcgggtgca gtagtacgaa gcatgaacct tatccgccac ctggcccgcg
     4741 cctattatcg cgacccgcag cggagagctt gtcattgcac ttttcacaaa gacctcttag
     4801 caggtacgca accaggccaa cgaatcacgg tacgcctggg caggatcttc tgcccggata
     4861 cgcccttcat acaccacata accctgatag ttatcggcgc gcagctgttc aaacagcgcg
     4921 tggaaatcga gggtgccact gcccggctgg taacgatggt tatcggcaat atgcacatga
     4981 ccgagcaagt cacggttgtc gtgcagcgcc tgcgccaggt tatcttcttc gatgttcatg
     5041 tgatagaaat cgccgataat ctgcacatgt ttaagattgt tttcgacgat gtaacgacga
     5101 gcatcggcga gggtgttgat catatgatcc tgatagcggt ttaacggttc gagatacacc
     5161 acggttccgg tacgcgcggc gacctgttcc agtacgcgca gggaatcact caccattttg
     5221 cggtcgccgt ccaggctacg cggcgaggtc atcggcggta agcggaaggt aaacatgccc
     5281 cacgctgccg gaacgacgat acctttaccc cccacttctg ccagcgcctc gagaatgcgt
     5341 tcgatctgct ttaagccatt aagacgacgc tcttcgataa agtcaccaat ccagccgtca
     5401 tagccgccgc aggcggtggt cactggtaaa ccggtttctt tgatcgccgc tttgacttct
     5461 tcgatgttgt tgaccagcag tttgccgtca atctcaaaac catcgaagcc catctctttg
     5521 atataacgaa atctctcgcg gatattttcc ggaaagaatg cctgattctg tgtgccgatt
     5581 ttcatttttt tattcctttg aattaaaacg taacgcccat tttgatgctc tgttccggat
     5641 gctgatcgac atactgcatg tagctttccg ggctgttggc aaaggtcact accgggtcga
     5701 ttaaatcttc gcaattgaga taaccgttca tcagcagttc ccagcaggtt tcttcaatac
     5761 gcttacggct ccagcgcgga taatccgggt tcggttcgct gcacgcgcgg gagaaaacaa
     5821 ttttggcgtt attgaaatgc gcttcgcgtc cgaggttaaa accttcagca aatggtttgg
     5881 caaacgcgac ataggagatg gtgccgccgt aggccaggcc gcgcagcgcc gattgcagcg
     5941 cgtcggcgta accgctggtt tcgataatca catcggcccc ctgcttgccg gtcagcgttt
     6001 tgatctcttt gcctacgtca gtgccaatgg ggttaaggca gaaatcagca ccgtggcgac
     6061 gggcaatatc acagcgatgg gcgataggat cgacgccaat taccaccgat gcgccagcgc
     6121 gtttcgccag ttggatggca atttgaccga tcgcgccaag ccctaccacc acgacaaaat
     6181 cccctacgcg tacgttggca tcgcgcacgc cgctcatggc aaactgcgcc gggtcgtaac
     6241 agacggcgtt tttccaggag ctgccttgcg gcattttgcg cagcttgtag ttattcactg
     6301 cgttaatgat gaccgtctcg gagagcgggc cgtagccgca tacgctgtcg cccaccgcgt
     6361 agtctgtaac gtcgctgccg cactcgataa tgtcgccaac caccatgttg ccaagctgga
     6421 atttgccaaa ctcaatgccg cgcggcgcat ctgccggacg cggggtgaac atctgccact
     6481 cgccgttaaa gtcttcatcg ataaacgggc tggcggcgcg gaagtcgacc acttccgttc
     6541 cgtgtttcgg tgcgccgaaa cgcacgcgga tcttcacttc attggctaaa atcgcccgat
     6601 cttcatactc aaccagcgca gcaacacgcg gtgctgtggc tactaacttt ttcatgtaaa
     6661 actccttgta aagaaactgg ccgctttagc ccttcacgcc accggcggtc aaaccactct
     6721 taataaaacg ttccgaaagc gcatacataa tcaccaccgg caatgcggtc accagcgagg
     6781 ccgccatcat ccgtccccag atgtaatcag gcgtactgaa cagcgcgttc aggcccactg
     6841 gtaaggtgaa attgctggcg ctggagagga aaatcgacgc aaacagatag tcgttccacg
     6901 ccaccataaa gcagtagacg aacaccgata tcagcccgga catcgccagg ggcacggtga
     6961 tacggaagat gatttgcaga cggttgaggc catccatcat cgccgcttct tcgatctcat
     7021 cagggatggt gtcgaagtaa cttttcagca taaacacggc ggtgggcagg gtttgcgtca
     7081 ccatggtaat gatcagcgcc atctcggtgt cgtaaatacc aagcgcggtg atgattttga
     7141 acagaggcac caccagcaaa atgccagaga acatatacac cgtgtaaaag ctggcgttga
     7201 tggtcatccg gcctttaaag cgcaggcggg aaagcgcgta agctccgaga atgccgagaa
     7261 ataccgccac cacggaggaa accaccgaca ccaccatgct gttacggaag tagtcgacaa
     7321 acgggaaaat catcgggtta aaaatgtcga cgtaatgctc cagcgtccac tgttgcggca
     7381 gcagcgtagg atgcagcgat atcgcctctt tcgcgctttt gaacgaggtc atcagcatca
     7441 caaaaaacgg aaacagcgtg atgatgagaa acagcgccag cccgcagtaa aaaccgatac
     7501 gactgagcgt gcgtttattt gttgccattg aggttcaccc gtttcctggt cagcagaatg
     7561 acagcgaaaa taatgatgaa gagcaccacc gagatcgccg ccgctttccc taagtcatta
     7621 aaggcaaagg cggttttgta gagatagaca ccaagaatat cgaccttggt tgtcagcaga
     7681 taaacatcag cgaacatgta gaacatccag atggtgcgca gggtcaccac cgtcgccagg
     7741 accggcataa ttgcgggcag cgtgacgatg cgaaaccgct gccaggcatt agcgccatcc
     7801 atctctgccg cttcgtacag cgatttgtcg atggtctgca aaatcgccag aaacgagata
     7861 aaggcatacg ggaagtagcg ccagatggcg aacagcacca ccagcacaaa gctactgccc
     7921 ggattatcaa accacagcgg tgcctgctca tagagatgca gaagatcgac gccgaggtag
     7981 ttcacaatgc cgtagccgtt gttgaacatg tatttccagg caaacaccag cgaaatggac
     8041 ggcgttacgt aggagaggat caccagcgaa cgcgcggttt tgcgcaggcg gaactcgcgg
     8101 ttgaaaaaca tcgccacggc cagcccgagc acggtgctgc ccgccaccac cagcgccgta
     8161 taccagaccg tcatccacag cgagtgccag aagccgggat cggagagaat gcgcacatag
     8221 ttactcaccc caacaaacgt tgactcgatg ttgggattga gcggcagacg taaaaaactg
     8281 atttcgatat ttgacaccat cggccacgcc accagaccgc ccagcagtaa taagctgggc
     8341 gcgagaagca gcagcgcaaa cggcatatcg gaacgacctg aaaaaagcct gttcattgcg
     8401 gattcctgtc ctgggtttag tgctgttcga tcagttcatc cagctttttc tggctcgctt
     8461 gcagcgtgcc agggagatcg gctttaccca cggtgacgtt atgcaccatg gagctcacca
     8521 caccagaacc cgtcacatcg cccatgcggg taaagttttt atcccctact gcgccaaaga
     8581 cctgaatatt tggcagttca ccgattagct gattcggtag ttcacccagc gccttaataa
     8641 cgtcgttgtc tttccaggtg gcggtagtga caaccgcttt attcaccggc agcgcagcac
     8701 ctggcgacat catcacccag tcggcaatgt tgtccgcctg ctccataaag gtgacaaatt
     8761 tttctgctgc ttcggtcttt tgtccggcgg taatggtcag cgaggccaac atgccgtaga
     8821 ccgcagagtt tttctcggtt ggcaccacga aaccgacgtt tttcgggtcg ccttctttaa
     8881 tcacagccgg aaggatatag gtggagtaaa tcgccatcgg cgcggtgccg ttcataaagg
     8941 cgtctttcac ttccatgatg tcgttagaac ccggcatggt gttggcagca aggtcgcggt
     9001 aataggtcag cgcctgcatc atctctggtg tatcaagggt gattttgcct tcggcgttaa
     9061 agacgttggc ctggttggat aacgcaaact gggaaaagga ttgttccgtc agcacgcttt
     9121 ctgccgtagg cagcgcaata ccgtattttt tattcgccgg gtcattcagt ttctgtgcaa
     9181 cgtccagcag ctgttgcctg tttttcggct cctccagccc cgcttttgcc agcacatctt
     9241 tgcgatacca gatgccgcca atccaggcgc tgacaggaac accggtccat gcgctaccat
     9301 cttcggtacg cacaatacgc agtacgccat cgtaaaacgc gccttcacca acgttgctga
     9361 tgaccgtggc aaccgctttg cgatcgataa gctgctcttt atccataact ttggcgtagt
     9421 catggctggt ttcgatcact tccggcagcg agccgctacg tgaaagggtg atgactttgg
     9481 tgttataagc gtcttcttcc acgggcactt gtttaacggt gatacctggg ttttcttttt
     9541 caaaacgggc aatcaatttg ctgataactg cctgacgctc ctgctccacc gaagaatgca
     9601 taaattcgat ggatacatgg gttttatttt cttctttaca gcctgaaatc agggcgcagg
     9661 aaaccagtgc tgataacagc acgatttttg atttaatcat gagatgttcc tttttattta
     9721 atccacataa cctgccaggg gcgcagcgtt atttcattac ccaatatatc aacttcacta
     9781 atcagatccc gaccatgcag atcagtaata tttacatgct gaatattttt actgacatta
     9841 aacaatccag tcagacaatt accatcagcg ttacttcttt gaatacgcat tacggatgaa
     9901 ttaaccgtat cgatggtaaa attattatcc ggatgaaact cgttatggct gcgacgaagt
     9961 gtaattaaac gcgacaactc atgatatacc gcatgcctta atgtagcttc atcgttcagt
    10021 tctcgtgtta tctcttcact gtgatatttt ttacggttaa tcgcacggtt atatccgagt
    10081 ttttcgacac ctgcgtaatc attacgcgag ccaagaatac tttgaatata tatcgcgggt
    10141 acaccgggga aacttaacaa aatcgcatgg gcaaggataa acctggcgca acgttcttca
    10201 tcgctactct cgcggcggct taacgcatcc atataggtga cattcatctc atagggactg
    10261 cgcgtgccgt cgggattatt tttccagttt actaatgcac cttcctgctg taatgcctcg
    10321 accagcgcta atatttcgct ttctggaagc aagccacgca gtgggttcaa cccgatgcca
    10381 tcgtgagagg cgaggaagtt aaaccaggtc gttttactgg agggcaagct cagactttgc
    10441 gcccactggc acagcgcctc gacgttctgt ttttgcaccg catgtaacac cagcggcggc
    10501 agcgagaact ggtacaccat atgcgcttcg tcatcaccgt tgccaaagta agcaatgttg
    10561 tctttgtgcg gaacattggt ttcggtaatg atcactgtac ctggtgctac gtcatcaata
    10621 atcgaccgta acagcttgat aatcagatgt gttttttcca gatggatgca gcttgttccc
    10681 ggctctttcc acataaagcc aacggcatcc aggcggacat actcagcacc tttctcaagg
    10741 taacagagta aaacatccac catcgccagc aacacttcag ggctacggta attcaggtca
    10801 atttgagtca ctaaaggtgg tccataaatg gcgcgtggaa ttatcatcca tttgaaacgg
    10861 cgttaatagc ggtaacgctc gcggacgggt gacggcactg agatcggttt tcggatcaac
    10921 cgcaataaag aaatcttcaa aacccggaag ctgttgtaaa tagtttttaa accattcact
    10981 ttttgccgac atatggttgc agacaaaatc aaacattaaa tgactgcatt caccgagttg
    11041 ctgaatatcc tgccactccc ccgcttcact ggcgacctga tgataatcaa ttaccgaaaa
    11101 tccatcatca gatgaccatg ggtaaaaagg taataaatga acatgtgaaa aaatgctttg
    11161 cagccattga tggtagaact gattaaatat gggtagtggt tttaaatcat tactgtgaaa
    11221 ttgatcggca taggtaatta acacgacatc actttcatcc cagtgttttt tacgtcgctg
    11281 tgtaattaat cgtttcgcac tctcaagacg cgttacaagt ttctgtaaat gagttgcggt
    11341 aaatgttcca ccgtagattt cgtccaggta atccgtaatt ttctgtttca tgttgccttt
    11401 ccgtggtagc ggtcccatga agcgagagac tactccgcag aaaataaact gtcaattgag
    11461 cacaagggtt ttagtcacgc aggaaatcat tttttaccag ggaaaaagcg tatttgtgag
    11521 ctgacgcaaa taatctatag ggatgtaaat aaggtgtgac agaaaaaaaa cggcgcataa
    11581 gcgccgctca tggtgaattc ttttaacctt tgaccttcgg cattgcgatg tctttcaggc
    11641 caccggcgtt ctcaacgtgg gtatatccca tctcgctaag gatctctttt gcctgcccgg
    11701 actggcgtcc ggcattgcaa tacactttca cggtgtcgtt tttatccgga acggcggtgg
    11761 caatgcgctc tttcacttcc ttcaggggaa tattgatggc cccctgaacg tgctcttgct
    11821 gatactgctc tggaacacga acatcgatcc agtgttcagc ggcgaaaacg ggcagtgaaa
    11881 acaccagtgc cagagcaagt aagccttttt ttaaacatgt aagcgtcctt ctatggatga
    11941 aaattgccat ccattatacg cctgagattt aacaattttt gcgaaactcc cctcaccttt
    12001 tataacgctg tgccagttta ttagcagccc gacttaacag cggttccagc gccacagcca
    12061 gcaacatttt cagcggtcgg cgagcaactg attttatcgc ccagcccgcc acacccgccg
    12121 ggccatagcg cagtgcggta agaagtacca gcttgcctgc taatttgaaa ccaggcttca
    12181 ccttttgccc ggcctgttgc cagcgagtat tcataacttt cctcacagtt gacggaaacg
    12241 gctacgtaac gtgaaagtat cggaagtgac ataacgttcc atctcgcgta aacgcgtttc
    12301 acttgccgcc agttcacggt cgacttcatc cagcaattcg ctgctggaag gtagctgctc
    12361 accaaaggcc atgttgtccg gcattggatc aagcgcaaat gacaaaatga tgtaagcaac
    12421 cagggtaaac agcgccagac cgaagaaaat cgacagcacc accaggatac gcaccagttt
    12481 taccggtaca tcaaaatagt tggcaatccc ggcgcagacg ccgcggacca tgccctgctg
    12541 tggaatacgc cataattttt tattgagatt aatgcccgcc attagcgatc cctccagttc
    12601 ggatgttctg catcaagaat agattccagc gcctgaatac gttcgcgcat ccgttttgct
    12661 tcatcagcca gttgcgctaa tcgctgctgc tcactttgcg acaattcact gcgaccagaa
    12721 cgattgctgt aatgcagcca taaccagatc ggtaaaacaa acagcacaaa aatggttaac
    12781 ggaatagcca gaaatagcgc gctcataagt actccttaga cgatgagcgg cggcgcgttc
    12841 ggacgccgcc tggatgttat tattgattgt cttgcttcat tttggctttt aattgtgcca
    12901 gttgttcgct gattgcatca tcggctttca gttcggcaaa ctgatcgtcc agcgattttt
    12961 gtttaccgaa gctgtggctt tctgcttccg cttccatctg gtcaatacga cgttcgaaag
    13021 attcgaaacg agccattgct tcatccagtt tgccactgtc cagctgacga cgcacatcgc
    13081 gcgacgagtt tgccgcctga tgacgtaaca tcaatgcctg ctggcgagcg cgtgtttcgc
    13141 tcaatttgtt ttccagctcg ccaatctctt tcttcatgcg tgccagcgta tcgtccacca
    13201 gcgtcacttc atgttccagg gacttaatca gatcggtcag tttctgtttt tcaattaacg
    13261 ctgcacgtgc cagatcctct ctctctttca gcagcgccag ttcggctttt tcctgccatt
    13321 caacttcacg cgccgacgct tgttcaatac ggcgagtcag ctgtttcttt tctgccaacg
    13381 cacgcgccga agtagaacgt acttcaacca gtgtatcttc catctcctgg atcatcagac
    13441 gcaccagttt ctgtggatct tccgctttct ctaacagagc gttgatgttg gcattcacga
    13501 tgtcggcaaa gcgagaaaaa atacccataa tgttgtcctc ttgatttctg cgttcaggat
    13561 tgtcctgctg aactgttaat acaatttgcg tgccaatttt ttatcttttt gatttataaa
    13621 gatctgattg aagaatcaac agcaacatgc caggatgaat tagcgaatta cactaacaag
    13681 tggcgaattt catcatggcc gaatacaaag ataatttact tggtgaggcg aacagctttc
    13741 tcgaagtgct ggaacaggtt tcgcatctcg caccgctgga caaaccggtg ctcatcatcg
    13801 gcgaacgcgg caccggtaaa gagttgattg ccagccgcct gcattatctc tcctcccgtt
    13861 ggcaagggcc gtttatttcc cttaactgcg cggcgttaaa tgaaaatctg ctggattccg
    13921 aactgtttgg tcacgaagcg ggggcgttta ccggtgcgca aaaacgtcat cctgggagat
    13981 ttgaacgtgc cgacggcggt acgctatttc ttgatgaact cgctacggca ccgatgatgg
    14041 tgcaggaaaa attattgcgt gtgattgagt acggtgaact ggagcgcgtt ggcggtagcc
    14101 agccgttgca ggtgaatgtg cggttggtat gcgcgacgaa tgccgatctc ccggcgatgg
    14161 tcaatgaagg tacttttcgc gctgacctgc tcgaccggct ggctttcgat gtagtgcaac
    14221 tgccgcccct gcgcgagcgc gaaagcgaca ttatgctgat ggcagaacac tttgccattc
    14281 agatgtgtcg ggaaatcaag ctgcctctgt tcccggggtt tacggagcgc gccagagaaa
    14341 cattgctgaa ttatcgttgg ccgggaaata ttcgtgaatt gaaaaacgtg gtggaacgtt
    14401 cagtgtatcg ccacggcacc agcgattatc cgcttgatga catcattatt gatcccttta
    14461 aacggcgtcc acctgaagaa gccatcgccg tttcagaaaa tacctcgctt ccgacgctgc
    14521 cgctggattt acgcgagttt cagatgcagc aggaaaaaga gttgctgcaa ctcagtttgc
    14581 aacaggggaa atataaccag aagcgcgcgg cagaattact gggtttaact tatcatcagt
    14641 tccgcgcgtt gttgaaaaag caccagattt agcgaagttg agcaatacaa cttgccggat
    14701 gcggcgtgaa tgctttattc ggcatacata atcaatacgt cgtttgcagg cctgataagc
    14761 gcagcgcatc agacattatt gcgttgggct attaatcgct cagcgcatcc tgcaaaattt
    14821 tcattgccgc atcgaattgc gcatccggga tggtcagcgg atacaggaag cgaatcacgt
    14881 tgccgtatgc gccacaggtc agcaggagca gcccctgcgc cagcgcgcgt tgctggattt
    14941 tctgtgcaat cgccgctgac ggctcgcccg tttgcggatc gttaaactct gccgcaatca
    15001 tcgaccccag gccgcgtacc gcagcaatgg ccggaacgct ttctttggca tcaatcaacg
    15061 tgtttttgag acgctggccc agttgattcg cgcgttcgca gagtgattct ttgtcgataa
    15121 tattgagcac cgcgtgcgcg gcagccaccg ccagcgggtt cccggcgtag gtgccaccca
    15181 acccgcccgg cgcgggcgcg tccataatat tcgcgttacc gaccacgccc gaaagcggca
    15241 tcccgcccgc gaggcttttc gccatcgtca ttaagtccgg cttatcggcg taatgatcca
    15301 tggcaaacag tttaccggta cgcgcaaagc cgctttgcac ttcatcggca atcatcacaa
    15361 taccgtgctc gtcgcacagg cggcgaatgg cggcaaccag ctcttttggc gcaacgttga
    15421 aaccgccctc gccctgcacc ggttcgaaaa taatggccgc gacctgcttc gcttcgatgt
    15481 ctgatttaaa caagcgttcg atggcgtcga gggagtcctg tgttgaaatg ccgtgtaaat
    15541 ctgacggata aggtacgtga tacaccgaac cagggaacgg gccgaagccg attttgtacg
    15601 gcgcaacttt tccggtcagc gccatggtca tatatgtacg accgtggaag ccaccgctaa
    15661 acgcaatcac accaggtcgt ccggtatggg cgcgagcaat tttcaccgcg ttttccaccg
    15721 cttccgcgcc ggtagtgaag aatgcggttt tagcctgccc gctcactggg gcaagggcgt
    15781 taattttctc cgccagcgtg acgtagcttt cgtacggcac aatctgatac gccgtgtggg
    15841 taaactgttg caattgctgc tccaccgccg cgaccagatc agggtggcga tgcccggtat
    15901 tcagcaccgc aatgcctgcg gcgaaatcga tgtattcgtt gccctctacg tccttcagcg
    15961 tagcgttttc tgccgactgg gcgaagaagt tacacatcac gccaaccccg cgtggggtag
    16021 cagaaagacg acgctgatgg aattcattgt tgctcatgtt tcacaccttt tgactcttca
    16081 ctgttaatcg gaaattaaac gcccagacga tcgcgcaggc tgtaataagc cgcgcccatc
    16141 gcggtaaatg gcacgcgcag cgtgcgcccg ccggggaacg ggtaatgcgg cagattggcg
    16201 aaggcatcga aacgctcggc gtcaccgcgc agcagttcag caatcaaacg cccggcaaga
    16261 tgggtacagg tgacgccgtg gccgctgtaa ccctgcatgt aatagatgtt ggtatcaagg
    16321 cggccgaact gcggcatgcg cgacaaggtc agcaggaagt tgcccgtcca gcggtaatca
    16381 attttcacgc ccttcaactg agggaaggtt tttagtagtt tcggtaccac aagacgctca
    16441 acgtcatccg ggttgcgtgc accatacact acacccccgc cgtacagcag gcggttgtcg
    16501 gcggtaagac ggtagtaatc aagcagatag ttacagtctt ccacacagta gtttttcggg
    16561 atcagcgaac gggctaaatc ttccgacagc cgttcggtgg tgatcacctg cgtgccgcac
    16621 ggcatgctgc gtttcgctag ttccggctct actttatcgc ccagatacgc attcccggcg
    16681 acaatcacat acttcgccgt cacctgacca ttggcagtcc gaaccacggc tggcgtggtg
    16741 tgctggatct gcgtcacggc ggaaagctca tacacgcgcc cgccgttcag gcggatggca
    16801 tcggcttcac caatggcgag gtttagcggg tgaatatgcc caccgctgtg atccagcagc
    16861 gcaccagtgt agcgatcgct ggcgacctcg cggcgaatag cgttggcgtc cagcagctcc
    16921 agctgtttat tgccgtagcg ttcccagttc tctttctgct cttcgagtgt ggcgagttgt
    16981 ttatcattca tcgccacaaa cagaccgccg gggcggtagt cgcagtcaat ctgataacgt
    17041 ttgatacgtt cgcggatgat ctcgccaccc tcgaacatca tgctgccgag catacgggcg
    17101 gtgtccatgc cgtagctttt ttcgatcacg tcgatgtcgc ggctgtagga gttcacaagc
    17161 tgcccgccat tgcgcccgct tgcgccaaag ccgatgcgtg aggcttcgag aaccactacg
    17221 tcaaagcccg cttccgccag atgcagcgcg gaggagagcc cggtatagcc gccgccaacc
    17281 acgcaaacgt cgcaggtgat tgactcattc agtgtgtcga atggtgcata tttattcgca
    17341 ctggcggcgt agtaactgct ggtatgttcg gtcatttcag gcctccaggc ttatccagat
    17401 ggttttcagt tcagtgaatt tttcaagggc atgcagggat ttgtcgcgac cgttgccgct
    17461 ctgcttatag ccgccaaacg gcacggtcat atcgccgtcg ttgtagttat tgacgaagac
    17521 ggaaccggct ttcaggcgtc ggctcatgcg gtgcgcgcgg gagaggtcgc gcgtccatac
    17581 cgccgcgcca aggccgtact ggctgtcgtt ggcaagctgc agcgcctgat cttctgatgt
    17641 gaaacgggtg accaccagca ccggaccgaa aatctcttcg cggcttaagg acgcattcgg
    17701 gtccacatcc acgaagatgg tcgggccgat ggccgctgcc agcccggcgt tgcgaccatc
    17761 cagcagcagt tgccccttgc cttcgccttc acgaataaag gtgtgtaccg agtcagcgtg
    17821 agcgctgtcg attaaggtgc ccatggtggt tgcgggatca agtggatggc ccggctgcca
    17881 gttttgcgcc tgctgtttta acagggctaa gaattcatcg gcgatgctct cttccagtaa
    17941 caaacgcgtt ccggcgatgc acacctggcc ctggttgtag aagatgcctg cggcggtggc
    18001 gcttgccgct ttttgcagat ccggacaatc ggcgaaaacg atgttggcgc ttttgccgcc
    18061 cgcttccagc cagacacgtt tcatgttgct atcacccgcg tctttcagca gttgtttacc
    18121 cgtacgcgtg gagccggtaa aggcgatggc atcgatatcg ttatgacgcg aaagcgcctg
    18181 ccccgcttca tgaccaaaac ccgtcaccac gttcaacaca ccatccggca agcctgcttc
    18241 tttcgccagc cccgcgagac gaatcgcact gagcggtgat ttttcagacg gttttagaat
    18301 cacgctgttt cccgccgcca gcgccgggcc gagtttccag caagtcagca acagcgggaa
    18361 gttccacggc acgatggcgg caatcacgcc gaccggttca cgcacgatca tcgccagctc
    18421 atggctactg gtggtcgcca cttcgccata cactttgtcg atcgcttcgg cgtaccagcg
    18481 aatggcgcgc gccgcgccgg gaatatcatc acgcagactg tgacgaatcg gtttgccggt
    18541 gtcgagagtt tccagcagtg ccagctcttc ggcgtgggct tccattaaat cggcgagttt
    18601 attcagtacc gctttacgtt tagccggaga agagagtgac cagtcgccgt gttcaaatac
    18661 gccgcgtgct gcgctcaccg cacggtcgat atcgacgctc ttgccgcggg caattttcgc
    18721 cagcggtgcc tgggtgaccg gatcaacggt ttcaaaggtt tcattttccg ccgcagcagt
    18781 atattcaccg ttaataaata agcggttttc aatggcgaga cttaacgctt tatcctgcca
    18841 gtaagccaga tgatgaaaat tcattatgac tcctgtttca cgtctatcag atatatgcag
    18901 tttgtgaacg tcagggcgca gagatccccg ggctgcatgc ccgtttattt ctggcgacat
    18961 tctttatgga ttgccgggcg cattgcgctt acccggctta cagaacaaaa agattaaaac
    19021 gtggtgggcg tatgggcgct gataattcgg caaatacctg ccgacgtatt actgaaactg
    19081 tgcgggatgc cggtattaat ggcataactt tgccccgcga cgaggtggta atcctgacca
    19141 ttaatcgtca gaacaatttc accttccagt actgtgccta tttcctcacc ctgatgctta
    19201 attctttccc cagtggttgt gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga
    19261 ttcgggttac cgttatgaac cagcttcatt gacacaccct gactgcccat ctcaattaag
    19321 tcgtcctgat taatgacgac ctgcggctca tcaggttttt ccggctcgga aaagaattcc
    19381 gagagtgaca gaccatacac cttcagcagc ttttgcagcg tactgatggc agggctgact
    19441 ttatcttgtt ctatcgtact gatagcactg tgagtcagcc cggagagttc ggcggcacga
    19501 cgttgtgaaa gcccctgctg caggcggatt tccgacaagc gttttcctgg cgccagtccc
    19561 tcatcactca tatttgcatt tccttaaact gtagtggtca gagtcgctgt ttttcagcga
    19621 tatggtgctg ccaagcggtg ataaagccct cgaacaatat acgcgaaagc gcgtactcgc
    19681 tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg atgacgctaa
    19741 ccgcctcaac taagccatcc ggcgagcgag cttcaacacg caaccgtggg ctaacgacct
    19801 tcgccccttg tccatgtaga gagtttaccc aaaagttgct acattcaggt aacaacgcag
    19861 acagtaatcc cccctcttca acctgaactt catgcgacgg tgcatattgc tgttccaccg
    19921 gcagttcggg atcttcccga tgttccagca attcaggctg ttcgcacagc ttgcgatgca
    19981 acgaaccacc ggttgccacc accagttctt gtaaaccccg gcagatggcg aaaatgggga
    20041 tgcgcctttc gagtgcggca ttaattatcg ccatgctcag aagatcacgc ccgggatcgg
    20101 cgtcaggctc atcgccgttt tcaccatata ggtgcggctg cacattgctg ggactactag
    20161 gaagataaat gccatcgagt ttcggcaaaa gttgttcaag taacgacggt tccgccagcg
    20221 catgtggtag cgcaattggc aagccgcctg cgtggatgat ggcattcagg tacttttctt
    20281 gcagagtctg ggtcgcatga cccttaagcc tgttcctgca cattacgaca ccgataaccg
    20341 gattgttcat tatattttcc acgctcaatc tcacaaagtg gactaaatta tcgccattac
    20401 tgcgctttaa atgaccttta tgttcaatat tttttcaatc tagcagtggg ttttcatttt
    20461 tgaaaactca atttaacatt tgacaaacat ttagtttgca tacagattcg aatgatggtc
    20521 attatatttt acgctttgat aacgagcgga aaacaaacca aaggcgaaga atcatggaaa
    20581 ccaatatcgt tgaagtagag aactttgttc agcagtcaga agagaggcgg ggtagcgcct
    20641 ttacgcagga agtgaaacgc tacctggagc gctacccgaa tacgcaatat gttgatgttt
    20701 tacttactga tttgaatggt tgcttccgtg gaaaacgtat ccctgtttca agcctgaaga
    20761 agctcgagaa agggtattat ttcccggcct cggtatttgc aatggatatt ctgggcaacg
    20821 tagtagaaga agcaggtctg ggtcaggaaa tgggcgagcc ggatcgtacc tgtgttcctg
    20881 ttctcggttc cttaactcct tctgccgccg atccagagtt tatcggtcag atgctcctga
    20941 ccatggtcga tgaagatggc gctccctttg acgttgagcc gcggaacgtt cttaaccgcc
    21001 tgtggcagca gctgcgccag cgcggattgt tcccggttgt agcggtagag ctggagttct
    21061 atttactgga tcgccagcgc gacgctgaag gggatctgca accgccctgc gcgcctggca
    21121 ccgatgaccg caatacacaa agccaggttt actccgttga taacctcaac cacttcgccg
    21181 acgtgctcaa tgatattgat gaactggcgc agttgcagct gattccggca gatggcgcgg
    21241 tcgctgaggc ctcgccgggt cagtttgaaa tcaaccttta ccatactgat aacgtgctgg
    21301 aagcctgcga tgatgcgctg gcactaaaac gcctggtgcg tctgatggca gaaaagcata
    21361 agatgcacgc cacttttatg gcgaagccgt atgaagagca cgcgggcagc gggatgcata
    21421 tccatatcag tatgcaaaat aatcgtggcg agaatgtgct ttctgacaca gaaggcgaag
    21481 attcgccgct gctgaaaaag atgctcgccg ggatgattga cctgatgccg tcgtcgatgg
    21541 cgttgctggc accaaacgtg aactcgtatc gccgcttcca gccgggaatg tatgtgccga
    21601 cgcaggcgtc gtggggccat aacaaccgca ccgtcgccct gcgtattccg tgcggcgacc
    21661 gtcataatca ccgcgtggaa tatcacgtgg cgggtgccga tgccaaccca tatctggtga
    21721 tggcagcgat ttttgccggt attttgcatg gccttgataa cgagctgccg ttgcaggaag
    21781 aagtcgaagg caacgggctg gaacaggaag gcttaccctt cccgattcgc cagagcgatg
    21841 ccctgggtga gtttatcgag aatgatcacc tgcgccgcta tttaggcgaa cggttttgcc
    21901 atgtgtatca cacctgtaaa aatgatgagc tgttgcagtt tgagcgcctc atcacagaaa
    21961 cagaaattga gtggatgttg aaaaacgcgt aattcccctt gcggggttgt ttcatcggcc
    22021 tgagcggagc caggcctgac atttgctggt aatccagaca tttcccggat gtcgcgttat
    22081 ggagcgcagg cggcaacggg cttccgttaa cgattaagac tgtgacgagg aaatgagatg
    22141 atgccgatga ttaaatcccc gcatggcgag ggaggctgcg tatgcgcccc tcctgctact
    22201 gactggactc cgcccccttt gcttcccttg ctaaacaggt ttgatttcag gtcgacacga
    22261 ccgcaaacct tattacgcag gggaggcagc aattatggct attaattcac cactgaatat
    22321 tgctgcgcaa cccggcaaaa cccgtctgcg aaaatcactg aaattgtggc aggtggtaat
    22381 gatgggtctg gcctatctca cgccgatgac cgtatttgat acttttggta ttgtctccgg
    22441 cattagcgac ggtcacgttc cggcgtccta tttgctggcg ctggcgggcg tgctgtttac
    22501 cgctatcagc tacggcaaac tggttcgcca gtttccggag gccggttcgg cctataccta
    22561 cgcgcaaaag tcgattaacc cgcacgtcgg atttatggtc ggctggtcat cactgctgga
    22621 ttatctcttt ttgccaatga tcaacgtttt gttggcgaaa atctatctct ccgccctctt
    22681 cccggaagtg ccgccgtggg tgtgggtggt aaccttcgtc gccattttaa ccgccgcgaa
    22741 tctgaagagc gtcaacctgg tcgctaactt caataccctg tttgtactgg tgcaaatctc
    22801 catcatggtg gtgtttatct tcctggtggt tcagggactg cataaaggag aaggcgttgg
    22861 caccgtctgg tcacttcagc cgtttatcag cgagaacgcg cacctgatcc cgattattac
    22921 cggtgcgacg attgtctgtt tctcgttcct cggtttcgat gcggtgacca cgctttcgga
    22981 agagacgccg gacgccgcac gcgtgatccc gaaagccatc ttcctgacgg cggtatatgg
    23041 cggcgttatc tttatcgcgg cgtcgttctt tatgcagctg ttctttcccg atatcagccg
    23101 ctttaaagac ccggacgccg cactgcctga aattgcgctg tacgtcggcg gcaagctctt
    23161 tcagtcgatt ttcctctgca ccacgtttgt gaacacatta gcgtctggcc tggcttcaca
    23221 tgccagcgtg tcgcgtctgc tgtatgtgat ggggcgtgac aatgtgtttc cggagcgcgt
    23281 gtttggctat gtgcacccaa aatggcggac tccggcactc aacgtcatta tggtcgggat
    23341 tgtcgcgctg tcggcgctgt tctttgattt agtcaccgcg acagcactga ttaacttcgg
    23401 tgctctggtg gcgtttacct tcgtgaatct gtcggtgttt aatcatttct ggcggcgtaa
    23461 aggaatgaat aaaagctgga aggatcactt ccactatttg ctgatgccgc tggttggcgc
    23521 gctgacggtg ggtgtgctgt gggttaacct agagtcaacg tcactgacac tcggtctggt
    23581 atgggcttcg ttaggcggcg catatttgtg gtatctgatc cgccgctatc gcaaagtgcc
    23641 gctgtacgaa ggtgacagaa cgccggtgag cgaaacgtaa tagccggatg cgcggcgcat
    23701 tatcctgcct gagggtaacg cgccgcgcca ttaactataa aaagatgtat gacccggatc
    23761 acgagtgcta tcatccgccc ttatgcttag cgcattaacc ccaggggcaa ataatgaacc
    23821 acgatatccc gctaaaatat tttgatattg ccgatgagta cgcgaccgaa tgcgcagagc
    23881 ctgtcgcaga ggctgaacgc acaccgctgg cccactactt ccagttactg ctcacccgtt
    23941 tgatgaacaa tgaagagatc agcgaagaag cccaacatga aatggctgct gaagcgggga
    24001 ttaatcccgt acgcattgat gagatcgcag agtttttgaa tcaatggggt aatgagtaat
    24061 tatttaattg cagacgatcg gtcagtatcg ttgtctggat aaagcgttca cgccgcatcc
    24121 ggctaacaat gacacgcact gcagcgtgct ttaatgggtt tctcacgatc gtaccgctct
    24181 gctcctcgct aagttgttct gtgagttata gttatagaga aatcacgggt acgaatgttg
    24241 aacagatggc gacgccacct gttcaacgac caaccacacg gccctcatta cgacccctga
    24301 acacaaacgg taatggcaaa tgtcattaaa tattagtaag atagcctctt tacataaaat
    24361 cccttaatat tatgcgccag gtattatcgt ctcttttggt gattgctgga cttgtgagtg
    24421 gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga tatccgcgac agcggttttg
    24481 tctattgcgt cagcgggcaa gtcaacacct ttaacccatc caaagcgagc agtgggttaa
    24541 ttgtcgatac ccttgccgcc cagttttatg atcgactgct ggatgtcgat ccctatacct
    24601 atcgcctgat gccggaactt gccgaaagct gggaagtact cgacaacggc gcgacctatc
    24661 gcttccacct gcgtcgcgat gttccgtttc aaaaaaccga ctggtttact cccactcgta
    24721 aaatgaatgc cgacgatgtg gtgtttactt tccagcgaat ttttgaccgc aacaacccgt
    24781 ggcataacgt caacggcagc aacttcccct acttcgacag cctgcaattt gccgataacg
    24841 tgaaaagcgt ccgcaaactg gataatcata ccgttgagtt ccgactcgcc cagccggatg
    24901 cttctttttt gtggcacctc gcaacccatt atgcttcggt catgtcggca gaatatgccc
    24961 ggaagttaga gaaagaagat cgccaggagc aactcgaccg tcaaccggtc ggcaccggtc
    25021 cgtatcagtt gtcggaatac cgcgccgggc aatttattcg cctacaacgt catgatgact
    25081 tctggcgcgg taaaccgtta atgccgcagg tagtggtgga tttaggctcc ggcggcaccg
    25141 gacgtctgtc gaaactcctg accggggaat gcgacgttct ggcctggcct gctgccagcc
    25201 agctatccat tttgcgtgac gacccgcgct tgcgtttaac gctgcgtcct gggatgaacg
    25261 tcgcctatct ggcatttaac accgccaaac cgccgctaaa taatcccgct gtccgccatg
    25321 cactggcact ggcgattaat aaccagcgcc tgatgcaatc catctattat ggtacggctg
    25381 aaacggcggc ctctatttta ccgcgcgcct cgtgggccta tgacaacgag gctaaaatta
    25441 ctgaatacaa tccggcgaaa tcgcgcgaac agttgaaggc gttggggctg gaaaacttaa
    25501 cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa ccccagtcca ctgaaaactg
    25561 ccgaactgat tcaggcggat atggcgcagg ttggcgtaaa agtggtgatt gtgccggtag
    25621 aaggtcgctt tcaggaggcg cggttgatgg atatgagcca tgatctgacg ttatccggtt
    25681 gggcgacgga cagtaacgac ccggacagtt tcttccgtcc gttactgagc tgcgcggcaa
    25741 ttcattcaca gaccaacctc gcccactggt gcgatccgaa attcgacagc gtgttgcgta
    25801 aggcgctctc ctcgcagcag ctggcagcgc gtattgaagc ctatgacgaa gcgcagagta
    25861 ttctggcgca ggaattacct attctgccgc tggcgtcatc attgcgtttg caggcctatc
    25921 ggtacgatat caaaggtctg gtacttagcc cgtttggtaa cgcctccttt gctggggtgt
    25981 atcgcgagaa acaggatgag gtgaaaaaac catgattatc ttcaccttac gccgcatttt
    26041 gttattgatt gtcaccttgt tcctgctgac cttcgttggc ttcagcttga gctatttcac
    26101 tccacacgcg ccgttacaag gcgcgtcact gtggaatgcc tgggtgttct ggtttaatgg
    26161 cctgatccac tgggattttg gtgtttccag cattaacggt caaccaattg ccgaacagtt
    26221 aaaagaggtc ttcccggcga cgatagaact gtgcatcctc gccttcggtt ttgcgctgat
    26281 tgttggcatc cccgtgggga tgattgccgg gattacgcgc cataagtggc aggataactt
    26341 gatcaacgcc atcgccttac tgggcttttc aatcccggtg ttctggctgg cgcttctgtt
    26401 gacactgttt tgttcactca cgctaggttg gttgcccgtt tcagggcgtt tcgatctgct
    26461 ctacgaagtg aaaccgatta ccggttttgc gttgattgat gcctggcttt cggactcacc
    26521 atggcgggat gaaatgatca tgagcgcaat ccgccatatg atattgcccg tgatcaccct
    26581 gtcggttgcg ccaacaactg aagtgatccg cctgatgcgt atcagcacta ttgaagtgta
    26641 tgaccagaac tatgtaaaag cggcggcgac ccgcggtttg tcgcgcttta ctattttgcg
    26701 tcgccacgtt ctgcataacg cgttaccgcc ggttattcct cgcctgggct tacagttttc
    26761 caccatgctg acgctggcga tgatcaccga aatggttttt agctggccgg gtttagggcg
    26821 ctggttaatt aacgccatcc gccagcagga ttacgcagcc atttccgccg gagtgatggt
    26881 gtgtggctca ctggtcatta ttgttaacgt gatttctgat attttgggtg ccatggctaa
    26941 ccctctgaaa cataaggaat ggtatgcctt acgatagcgt atacagcgaa aagcgcccgc
    27001 cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga cgcctctgcg atggtcggcc
    27061 tgtacggctg cgcgggactg gctgtactgt gtatttttgg cggctggttt gcgccttacg
    27121 gcatcgacca gcaatttctc ggttatcaat tattgccgcc atcatggtcg cgctatggcg
    27181 aagtttcttt cttcctgggg actgacgacc tcggacgtga tgttttaagc cgtttactga
    27241 gcggagctgc gccaaccgtg ggtggcgcat ttgtggtgac gcttgccgcg acgatctgtg
    27301 gcctggtact gggcaccttc gccggggcga cacacggcct ccgctcagcg gtgcttaacc
    27361 atattctgga taccttactg gcgatccctt cgctgctgtt ggcaattatc gttgtagcgt
    27421 ttgccggacc gagtttgtcc cacgctatgt ttgctgtctg gctggcattg ctgccgcgta
    27481 tggtacgttc gatttacagc atggtgcatg acgaactgga aaaagagtac gttatcgccg
    27541 cccgtctgga tggcgcatca acgctgaata ttctctggtt tgctgtgatg ccaaacatca
    27601 ccgccgggct ggtcaccgag atcacccgcg cactgtcgat ggcaattctc gatatcgccg
    27661 cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc tgaatgggga gcgatgctcg
    27721 gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt catgctgcca ggtgcggcaa
    27781 ttatgattag cgtgttgttg gttaacctcc ttggcgatgg cgttcgtcgt gcgattattg
    27841 cgggggtgga ataatgccat tactcgatat tcgtaacctg accattgaat ttaaaaccgg
    27901 tgatgagtgg gttaaagccg tcgaccgggt aagtatgacc ttaaccgaag gtgaaatccg
    27961 cggtcttgtt ggtgaatccg gttccggcaa gagtttgatt gcgaaagcga tttgtggggt
    28021 gaataaagat aactggcgtg ttactgctga ccgtatgcgt tttgatgata tcgatttgct
    28081 gcgtctctcc gcacgcgaac ggcgcaaact ggttggccat aacgtgtcga tgattttcca
    28141 ggaaccgcag tcgtgtcttg acccttcaga acgtgtgggt cgccagttga tgcaaaacat
    28201 cccagcctgg acctataaag gccgttggtg gcagcgtttt ggctggcgca aacgccgtgc
    28261 cattgaactg ctgcaccgcg tggggattaa agatcacaaa gatgcgatgc gcagttttcc
    28321 ctatgagttg accgaaggtg aatgtcagaa agtgatgata gccattgcac tggcgaatca
    28381 accgcgtctg ctgattgctg acgaaccgac aaactcaatg gagccaacaa cccaggcgca
    28441 aatctttcgc ctgctgacgc gtctcaacca aaacagcaat accactattt tgcttatcag
    28501 ccatgactta caaatgctta gccaatgggc ggataaaatt aacgtgcttt actgcggtca
    28561 aacagtggaa accgcgccga gtaaggagtt ggtgacgatg ccacatcatc cttataccca
    28621 ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg ccgcataaaa gtcgcctcaa
    28681 tactctaccc ggcgctatcc cattgctgga acagttaccg attggttgtc gtctggggcc
    28741 acgttgcccg tatgcacaac gagaatgcat tgtgacgcca cgtttgacgg gggcgaaaaa
    28801 tcatctctat gcctgtcatt tcccgctgaa catggagaaa gagtgagatg atcgaaacgc
    28861 tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg gaccggttgg tttcgtcgtc
    28921 agaccgtaga agcggtaaaa cccttgagct ttacgctacg tgaaggccag acactggcga
    28981 ttattggcga gaatggttcg ggtaaatcca cactggcaaa aatgctggcg ggaatgatag
    29041 aacctaccag cggcgagtta ttgattgacg atcatccgct gcattttggc gattattcct
    29101 tccgtagtca gcgtattcgc atgatttttc aggatccttc gacctcgttg aatccccgtc
    29161 aacgtatctc gcaaattctg gattttccac tgcgcctgaa caccgacctg gaaccggaac
    29221 agcgtcgcaa acagattatt gaaacgatgc gtatggttgg gctgctaccg gatcacgtca
    29281 gttactatcc gcatatgttg gcacccggac aaaaacagcg tctgggtctg gcgcgcgcgc
    29341 tgatattgcg cccaaaagtc attattgccg atgaagccct cgcttcactg gatatgtcga
    29401 tgcgttcgca gttgattaat ctgatgctgg agttacagga aaaacagggc atttcgtata
    29461 tttatgttac ccagcatatc ggaatgatga agcacattag cgaccaggtg ctggtgatgc
    29521 atcagggcga ggttgtcgag cgaggcagca ccgcggatgt gctggcatcg ccgctgcatg
    29581 aactcaccaa acggctgatt gccgggcact ttggcgaagc attgacagcg gatgcgtggc
    29641 gtaaagatcg ctaattttct gaatcctcct tccagattat tcacctcatt ctgacaagag
    29701 attaaatctt gtcagaatga ggtgatggat aaaataaaat caaatgcgcg tgatttaaga
    29761 cgcaatctca ctttgcagga acgaaagctc tggcgatatc ttcgcagccg acgttttggt
    29821 gatttcaaat ttcgccgtca acatccggtg gggagctaca ttctcgattt tgcttgctgc
    29881 tcggcgcgcg tagtcgttga gctggatggt gggcagcatg atttggcagt tgcctatgat
    29941 accaggcgca ctagctggct tgagtcgcag ggctggaccg tgctgcgttt ctggaataac
    30001 gagattgatt gtaatgagga ggcggtgctg gagattattc tgcaggaact gaaccgccgg
    30061 tcaccctcac cctgaaaggg cgagggggcc gaccgagccg aatagctgtt gtggtgaaaa
    30121 catggaaaca gtgctggaga atattcggca aggtctgaac catcccagcc atcgccataa
    30181 aagggttagg ggctgtatga gcctgtttgt tgctggggta acaatatttg cacaatatgg
    30241 tcccctcgcc cctctgggga gagggttagg gtaaggggaa aagcgccccc cctgccgcag
    30301 cctgctccgg tcggacctgg caactatagc tactcacagc caggttgatt ataataaccg
    30361 tttatctgtt cgtactgttt actaaaacga cgaatcgcct gattttcagg cacaacaagc
    30421 atcaacaata aggattaaag ctatgggttt tctttccggt aagcgcattc tggtaaccgg
    30481 tgttgccagc aaactgtcca tcgcctacgg tatcgctcag gcgatgcacc gcgaaggagc
    30541 tgaactggca ttcacctacc agaacgacaa actgaaaggc cgcgtagaag aatttgccgc
    30601 tcaattgggt tctgacatcg ttctgcagtg cgatgttgca gaagatgcca gcatcgacac
    30661 catgttcgct gaactgggga aagtttggcc gaaatttgac ggtttcgtac actctattgg
    30721 ttttgcacct ggcgatcagc tggatggtga ctatgttaac gccgttaccc gtgaaggctt
    30781 caaaattgcc cacgacatca gctcctacag cttcgttgca atggcaaaag cttgccgctc
    30841 catgctgaat ccgggttctg ccctgctgac cctttcctac cttggcgctg agcgcgctat
    30901 cccgaactac aacgttatgg gtctggcaaa agcgtctctg gaagcgaacg tgcgctatat
    30961 ggcgaacgcg atgggtccgg aaggtgtgcg tgttaacgcc atctctgctg gtccgatccg
    31021 tactctggcg gcctccggta tcaaagactt ccgcaaaatg ctggctcatt gcgaagccgt
    31081 taccccgatt cgccgtaccg ttactattga agatgtgggt aactctgcgg cattcctgtg
    31141 ctccgatctc tctgccggta tctccggtga agtagtccac gttgacggcg gtttcagcat
    31201 cgctgcaatg aacgaactcg aactgaaata atcgttctgt tggtaaagat gggcggcgtt
    31261 ctgccgcccg ttatctctgt tatacctttc tgatatttgt tatcgccgat ccgtctttct
    31321 ccccttcccg ccttgcgtca ggataacgat ttcctttacg accaaggagc gcccatggaa
    31381 caacgccaca tcaccggcaa aagccactgg tatcatgaaa cgcaatccag tactacggag
    31441 tatgacgttc tgcctctggt cccggaagcc gcaaaggtca gcgatccctt tctgctcgac
    31501 gtgatccttg atgaagagac gctggccccc ttcctttcat ggctggtccc tgcgcgcgtt
    31561 cttgcagtgg agttgttccc tgaccagctt accgtgaccc gttcacaaac cttcaccgct
    31621 tatgaacgct tgtcgacggc cttgacggtt gctcaggttt gcggcgtcca gcggttatgt
    31681 aactactatt cggcgcgact tacgccgctc cccggacctg attcctccag ggaaagtaat
    31741 catcggttgg cacaaatcac gcaatatgcc cgccaactgg ctagctcgcc ttctattatc
    31801 gacaaccgat cgcgccagca tctgaatgac gtcggtctta ctgtctggga ctgtgtgatc
    31861 attaaccaaa tcattggttt tattggcttt caggcgcgga caattgcgac atttcaggct
    31921 tatctcgggc atccggtacg ctggttaccc gggctggcga tacaaaacta cgccgacgcg
    31981 tcactgtttg ctgatgaatc aatacgttgg cgaagcagct atgaggtgga aaaactacct
    32041 gaagagcaca caaaaagttc aactgcagaa ctttgccaac tggccgaaac actctctctc
    32101 caccctattt cactttccct tctcgaaaag ttgttaaaca gcacacgggt caatacacag
    32161 ccggataatc agcttgcggc gttgttatgc gcgcgtataa atggcagtcc tgcttgtttt
    32221 tccacctgta tggattcatc aaatgaatat aaaaaaatca gcacccttct gcgcaagggc
    32281 gaaaatgaaa ttaaccgatg ggctgaccgt cattctgttg agcgcgctac cgttcaggcg
    32341 atacaatggc tgacccgagc acccgatcgc tttagcgccg cccagttcag ccctttactc
    32401 gaacacgaaa aatcatcaac gcagattatt aatctgctgg tatggagcgg gctgtgtggc
    32461 tggataaatc gcttaaaaat cgcgttgggt gagacatatt aaccttgccg cgtcagacag
    32521 attcgcgtaa aactgtcagc cgctctaatg gccaccaaaa tagacaatta tgtttcagga
    32581 caacccgctg ctagcgcagc ttaaacagca actgcattcc cagacgccac gcgctgaagg
    32641 ggtggtaaaa gccacagaaa aaggctttgg cttcctggaa gtcgacgcgc aaaaaagtta
    32701 tttcattccg ccgccgcaga tgaaaaaagt catgcatggc gaccgaatta tcgcggtgat
    32761 ccacagtgaa aaagaacgtg aatccgcaga gccagaagaa ctggttgaac cgttcctgac
    32821 tcgtttcgtg ggtaaagttc agggcaaaaa tgaccgtctg accatcgttc ctgatcatcc
    32881 acttttaaaa gacgccattc cttgccgcgc agcccgtggc ctgaaccacg agtttaaaga
    32941 aggcgactgg gcggttgccg aaatgcgccg tcatccgctg aaaggcgatc gttctttcta
    33001 tgctgaactg acacaataca tcacttttgg tgacgaccac tttgtaccgt ggtgggttac
    33061 ccttgcgcgc cataatctgg aaaaagaagc accagacggc gtcgctaccg aaatgctcga
    33121 tgaaggtctg gttcgtgaag atctgacctc gctggatttt gtcaccatcg acagtgccag
    33181 cactgaagat atggatgacg cccttttcgc taaggcgctg ccggatgaca aacttcagct
    33241 gattgtggcg attgccgatc caaccgcgtg gattgctgaa ggcagcaagc tggacaaagc
    33301 cgcgaaaatt cgcgcattca ccaactatct gcctggcttc aacatcccta tgctgcctcg
    33361 cgagctttct gacgatctct gctcactgcg cgccaatgaa gtccgcccgg tactggcatg
    33421 ccgcatgacg ctctccgctg atggcaccat tgaagataat atcgaattct ttgccgccac
    33481 catcgaatcc aaagcgaagc tggtgtatga ccaggtttct gactggctgg aaaataccgg
    33541 tgactggaag cctgaaagtg aagcaattgc cgaacaagtc cgtttgctag cgcaaatttg
    33601 ccaacgccgc ggcgagtggc gtcataacca cgcactggtg tttaaagatc gcccggatta
    33661 ccgctttatt ctcggtgaaa aaggtgaagt gctggatatc gtcgccgagc ctcgtcgcat
    33721 tgccaaccgt atcgtcgaag aagcaatgat tgccgctaac atttgtgcgg cccgcgtact
    33781 gcgcgataag ctcggttttg gcatctataa cgtgcatatg ggctttgatc cggcgaatgc
    33841 cgacgcgctg gcagcgttgc tgaaaacgca cggtctgcat gtcgatgccg aagaagtgct
    33901 cacgctggac ggtttctgca aactgcgtcg tgaactggat gcgcaaccaa ctggtttcct
    33961 cgacagccgc attcgtcgct tccagtcatt tgctgaaatt agcactgaac ccggtcctca
    34021 ctttggcctc ggtctggaag catacgccac ctggacttcg ccgatccgta aatatggcga
    34081 catgatcaac caccgtctgc tgaaagcggt tatcaaaggc gaaactgcga cgcgtccgca
    34141 ggatgaaatc actgtccaaa tggccgaacg tcgccgtctc aaccggatgg cagaacgtga
    34201 tgttggcgac tggttatacg cacgcttcct gaaagataaa gccgggaccg gcacccgttt
    34261 cgcggcggaa attgtcgata tcagccgtgg cggcatgcgt gtgcgtttgg ttgataacgg
    34321 cgctatcgcc tttattccgg cacctttctt acacgctgtg cgcgatgaac tggtttgcgg
    34381 ccaggaaaac ggcactgtac aaattaaagg tgaaacggtt tacaaagtaa ctgacgttat
    34441 tgacgtcacc attgccgaag tccgcatgga aacccgcagc attattgcgc gcccggtcgc
    34501 gtaatctcct ttcacggccc attcctcatg gatgggccgt ttatttcccc gctctacctt
    34561 cgttatatcc aggcagattt tttaactgac tttcgtttga aaactggcgt ttttccaaaa
    34621 ctggattaca aataaatata taaaaacaat gcgtttagcc gccgttaatt tcatgtaagt
    34681 catgttgttc cttttattta accgcggact ccgctgttaa ccggaggata tgcatcatga
    34741 aaaccgttag ggagtccaca acgttgtaca actttctcgg atcgcacaat ccatactggc
    34801 ggttgacgga aagcagcgat gctttgcgct tttctaccac cgaaaccaca gaacctgatc
    34861 gtacattgca gttatctgcc gaacaggctg ctcgcatcag ggaaatgacg gtcatcacct
    34921 ccagcctgat gatgagtctg accgtcgatg aaagcgatct ttctgtgcat ctggtaggac
    34981 gaaaaatcaa taaacgggaa tgggctggca acgcgtctgc atggcatgac acaccggcgg
    35041 ttgctcgtga tttatcacac gggctttcct ttgctgagca ggtagtttct gaagcacatt
    35101 ccgcaatagt gattctcgac agccggggga atatccaacg cttcaatcgg ttatgtgaag
    35161 attacacagg gttgaaagaa cacgacgtca ttgggcaaag cgtgtttaaa ctgtttatga
    35221 gccgtcgtga agctgcggca tccaggcgca ataaccgtgt attttttcga agcggcaatg
    35281 catatgaagt cgaactgtgg ataccaacac gtaaaggcca gcggctgttt ctgtttcgca
    35341 ataaatttgt ccacagcggc agtggcaaaa acgagatttt tttaatctgt tccggtaccg
    35401 acattaccga agagcgccgc actcaggagc gactgcgtat tctggcaaat accgacagta
    35461 tcaccggact gccgaatcgt aacgcaatgc aggagttaat cgatcacgct attaatcagg
    35521 cagataacaa taaagttggg gttgtgtatc ttgatttgga taattttaaa aaggtcaacg
    35581 acgcctatgg gcatttgttt ggtgaccagt tattacgcga cgtgtcattg gctattttaa
    35641 gctgtctcga acatgaccag gtgttggcgc gtccaggtgg cgatgagttt ctggtactgg
    35701 catccaatac ctcacaaagc gcgctggaag caatggcatc acgaattttg acccgcttac
    35761 gcctcccctt tcgtattggt ttaattaaag tttataccag ctgttcagta ggtatttcgc
    35821 tctctcccga acatggttcg gacagcgcga ctattattcg tcacgccgac acagcaatgt
    35881 acacagcgaa ggaaggcgga cgaggacaat tttgcgtttt tactccagaa atgaatcagc
//
LOCUS       DBKEKI010000029        35595 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.029, whole genome shotgun sequence.
ACCESSION   DBKEKI010000029 DBKEKI010000000
VERSION     DBKEKI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.029"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..363)
                     /locus_tag="ACUQPX_001396"
     CDS             complement(<1..363)
                     /locus_tag="ACUQPX_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HHV9056154.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAE"
     gene            complement(510..586)
                     /locus_tag="ACUQPX_001397"
     tRNA            complement(510..586)
                     /locus_tag="ACUQPX_001397"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(550..552),aa:Pro,seq:tgg)
     gene            complement(629..715)
                     /locus_tag="ACUQPX_001398"
     tRNA            complement(629..715)
                     /locus_tag="ACUQPX_001398"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(679..681),aa:Leu,seq:cag)
     gene            complement(736..811)
                     /locus_tag="ACUQPX_001399"
     tRNA            complement(736..811)
                     /locus_tag="ACUQPX_001399"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(776..778),aa:His,seq:gtg)
     gene            complement(870..946)
                     /locus_tag="ACUQPX_001400"
     tRNA            complement(870..946)
                     /locus_tag="ACUQPX_001400"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(910..912),aa:Arg,seq:ccg)
     gene            complement(1049..2434)
                     /gene="thrP"
                     /locus_tag="ACUQPX_001401"
                     /gene_synonym="yifK"
     CDS             complement(1049..2434)
                     /gene="thrP"
                     /locus_tag="ACUQPX_001401"
                     /gene_synonym="yifK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015194 - L-serine transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015195 - L-threonine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine/serine APC transporter
                     ThrP"
                     /protein_id="HHV9056155.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(2625..3365)
                     /gene="wecG"
                     /locus_tag="ACUQPX_001402"
     CDS             complement(2625..3365)
                     /gene="wecG"
                     /locus_tag="ACUQPX_001402"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HHV9056156.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(3368..4720)
                     /gene="wzyE"
                     /locus_tag="ACUQPX_001403"
     CDS             complement(3368..4720)
                     /gene="wzyE"
                     /locus_tag="ACUQPX_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HHV9056157.1"
                     /translation="MSLLEFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(4717..5796)
                     /gene="rffT"
                     /locus_tag="ACUQPX_001404"
                     /gene_synonym="wecF"
     CDS             complement(4717..5796)
                     /gene="rffT"
                     /locus_tag="ACUQPX_001404"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HHV9056158.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEQ
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5793..7043)
                     /gene="wzxE"
                     /locus_tag="ACUQPX_001405"
     CDS             complement(5793..7043)
                     /gene="wzxE"
                     /locus_tag="ACUQPX_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HHV9056159.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(7045..8175)
                     /gene="rffA"
                     /locus_tag="ACUQPX_001406"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(7045..8175)
                     /gene="rffA"
                     /locus_tag="ACUQPX_001406"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HHV9056160.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(8180..8854)
                     /gene="rffC"
                     /locus_tag="ACUQPX_001407"
                     /gene_synonym="wecD"
     CDS             complement(8180..8854)
                     /gene="rffC"
                     /locus_tag="ACUQPX_001407"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HHV9056161.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8832..9713)
                     /gene="rfbA"
                     /locus_tag="ACUQPX_001408"
     CDS             complement(8832..9713)
                     /gene="rfbA"
                     /locus_tag="ACUQPX_001408"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HHV9056162.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9732..10799)
                     /gene="rffG"
                     /locus_tag="ACUQPX_001409"
     CDS             complement(9732..10799)
                     /gene="rffG"
                     /locus_tag="ACUQPX_001409"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HHV9056163.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQGERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10799..12061)
                     /gene="wecC"
                     /locus_tag="ACUQPX_001410"
     CDS             complement(10799..12061)
                     /gene="wecC"
                     /locus_tag="ACUQPX_001410"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HHV9056164.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(12058..13188)
                     /gene="wecB"
                     /locus_tag="ACUQPX_001411"
     CDS             complement(12058..13188)
                     /gene="wecB"
                     /locus_tag="ACUQPX_001411"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HHV9056165.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(13244..14290)
                     /gene="wzzE"
                     /locus_tag="ACUQPX_001412"
     CDS             complement(13244..14290)
                     /gene="wzzE"
                     /locus_tag="ACUQPX_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HHV9056166.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(14302..15405)
                     /gene="wecA"
                     /locus_tag="ACUQPX_001413"
     CDS             complement(14302..15405)
                     /gene="wecA"
                     /locus_tag="ACUQPX_001413"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HHV9056167.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(15645..16904)
                     /gene="rho"
                     /locus_tag="ACUQPX_001414"
     CDS             complement(15645..16904)
                     /gene="rho"
                     /locus_tag="ACUQPX_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HHV9056168.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16989..17090)
                     /gene="rhoL"
                     /locus_tag="ACUQPX_001415"
     CDS             complement(16989..17090)
                     /gene="rhoL"
                     /locus_tag="ACUQPX_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HHV9056169.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(17231..17560)
                     /gene="trxA"
                     /locus_tag="ACUQPX_001416"
     CDS             complement(17231..17560)
                     /gene="trxA"
                     /locus_tag="ACUQPX_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HHV9056170.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            17691..18956
                     /gene="rhlB"
                     /locus_tag="ACUQPX_001417"
     CDS             17691..18956
                     /gene="rhlB"
                     /locus_tag="ACUQPX_001417"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HHV9056171.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            19090..20574
                     /gene="gppA"
                     /locus_tag="ACUQPX_001418"
     CDS             19090..20574
                     /gene="gppA"
                     /locus_tag="ACUQPX_001418"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HHV9056172.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGNFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(20621..22642)
                     /gene="rep"
                     /locus_tag="ACUQPX_001419"
     CDS             complement(20621..22642)
                     /gene="rep"
                     /locus_tag="ACUQPX_001419"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HHV9056173.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22729..23010
                     /gene="ppiC"
                     /locus_tag="ACUQPX_001420"
     CDS             22729..23010
                     /gene="ppiC"
                     /locus_tag="ACUQPX_001420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HHV9056174.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23056..24531)
                     /gene="ilvC"
                     /locus_tag="ACUQPX_001421"
     CDS             complement(23056..24531)
                     /gene="ilvC"
                     /locus_tag="ACUQPX_001421"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HHV9056175.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     CKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            24675..25565
                     /gene="ilvY"
                     /locus_tag="ACUQPX_001422"
     CDS             24675..25565
                     /gene="ilvY"
                     /locus_tag="ACUQPX_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HHV9056176.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(25562..27106)
                     /gene="ilvA"
                     /locus_tag="ACUQPX_001423"
     CDS             complement(25562..27106)
                     /gene="ilvA"
                     /locus_tag="ACUQPX_001423"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HHV9056177.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27109..28959)
                     /gene="ilvD"
                     /locus_tag="ACUQPX_001424"
     CDS             complement(27109..28959)
                     /gene="ilvD"
                     /locus_tag="ACUQPX_001424"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HHV9056178.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(29024..29953)
                     /gene="ilvE"
                     /locus_tag="ACUQPX_001425"
     CDS             complement(29024..29953)
                     /gene="ilvE"
                     /locus_tag="ACUQPX_001425"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HHV9056179.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSLSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29973..30236)
                     /gene="ilvM"
                     /locus_tag="ACUQPX_001426"
     CDS             complement(29973..30236)
                     /gene="ilvM"
                     /locus_tag="ACUQPX_001426"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HHV9056180.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30233..31879)
                     /gene="ilvG"
                     /locus_tag="ACUQPX_001427"
     CDS             complement(30233..31879)
                     /gene="ilvG"
                     /locus_tag="ACUQPX_001427"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HHV9056181.1"
                     /translation="MNGAQWVVHALREQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMVEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31882..31932)
                     /gene="ilvX"
                     /locus_tag="ACUQPX_001428"
     CDS             complement(31882..31932)
                     /gene="ilvX"
                     /locus_tag="ACUQPX_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HHV9056182.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32018..32116)
                     /gene="ilvL"
                     /locus_tag="ACUQPX_001429"
     CDS             complement(32018..32116)
                     /gene="ilvL"
                     /locus_tag="ACUQPX_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HHV9056183.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            32468..33988
                     /locus_tag="ACUQPX_001430"
                     /pseudo
     CDS             32468..33988
                     /locus_tag="ACUQPX_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(34013..34351)
                     /gene="maoP"
                     /locus_tag="ACUQPX_001431"
     CDS             complement(34013..34351)
                     /gene="maoP"
                     /locus_tag="ACUQPX_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HHV9056184.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMMRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            34470..35309
                     /gene="hdfR"
                     /locus_tag="ACUQPX_001432"
     CDS             34470..35309
                     /gene="hdfR"
                     /locus_tag="ACUQPX_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HHV9056185.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(35406..35481)
                     /locus_tag="ACUQPX_001433"
     tRNA            complement(35406..35481)
                     /locus_tag="ACUQPX_001433"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35446..35448),aa:Trp,seq:cca)
     gene            complement(35490..35566)
                     /locus_tag="ACUQPX_001434"
     tRNA            complement(35490..35566)
                     /locus_tag="ACUQPX_001434"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35530..35532),aa:Asp,seq:gtc)
BASE COUNT         8677 a         9744 c         8986 g         8188 t
ORIGIN      
        1 ttctgccaga aatgcacacc attcgtcatc gagcagcacg ccgttagtct ggaaactgtt
       61 acttatcttc ctgccagcac catatttcgc ctgtagcgcc acggcacggc ggtaaaactc
      121 cagcccgagt agcgttggtt cgccgccctg ccaggtaaaa gccacttcgt tttgcagttc
      181 gctggcggcg atatagtgac ggatatacgc ttccagcgtg tcatcgtcca tatgcgtgac
      241 tggcttttcg cggtaaaggg attgtttttc gagataaaaa cagtagtcac agttcagatt
      301 acaatcggaa ccgctcggtt tcgccatcac atgaaaagca cgcgttggaa cctgttgcag
      361 catggtcgct ccttgcctga agtcatcctt caatacgtta agggcgaaat cataccgtgg
      421 ggcaatgagt tattacgggc gtttattgcg aagggagggt gacgaaatgc acagaaaaca
      481 aaaaaccccg ccgaagcggg gttcaaaatt ggtcggcgag agaggattcg aacctccgac
      541 ccactggtcc caaaccagtt gcgctaccaa gctgcgctac tcgccgaaat actgcttttt
      601 gaatttttag ttcaattctt taaagtcgtg gtgcgagggg ggggacttga acccccacgt
      661 ccgtaaggac actaacacct gaagctagcg cgtctaccaa ttccgccacc ttcgcattgt
      721 cacaacttct aataatgggg tggctaatgg gattcgaacc cacgacaact ggaatcacaa
      781 tccagggctc taccaactga gctatagcca ccactacaaa tcttgttacg cggtattact
      841 accaccgcag ctcaagcgcc gggactaaat ggcgcgcccg acaggattcg aacctgagac
      901 ctctgcctcc ggagggcagc gctctatcca gctgagctac gggcgcttag cgccgttgcg
      961 ggggtggata atacggactt cccacccctc tgtctagtcc ctttttaaat aaattgcgcg
     1021 tttggttacg gtttgtgcgt tttgctgctt attcctccag tttatgcgct ttaccgtggc
     1081 gattaagccc aaaaacttta taaatcgccg tcaccgccag cataaagatg atgccgacaa
     1141 acagcgacat acgcgtatct tcattaaagt acatgccgat caaaacgcaa atcaggaatg
     1201 ccattgttac gtagttggcc cacgggaaca ggattgagcg gaacggatgg ctggcaatcg
     1261 ccgctttatg tgcacgccga aaacgcagct ggcttatcaa tatcacaaac catggcacca
     1321 tccccggaag cacgctggca ctgtagacgt agacaaacac acgctgcgga ttgggaatga
     1381 tgtagttcag gcatgagcca attagcagaa tagcaataga tactgccaca cccgcaaccg
     1441 gtacgccgtg acgggaaact tttgccattg ccgccggtaa ctgacggttt ttcgccagtg
     1501 cgtagagcat acgtccgcaa ctgtacatgc cgctgttaca gccagagagc gcagccgtca
     1561 gcaccacaaa gttgataatg cccgccgctg cggtaatacc gattttggca aaagtcagta
     1621 cgaacgggct gccgttgctg ccaatttcat tccacgggaa gatggtgacg ataacgaaaa
     1681 tcgcgcctac gtagaaaatc aggatccgcc acagcacttt gcctacggca ctgcgcagcg
     1741 tcacctgcgg attcttcgct tcaccggctg taatgccaat cagctccacg ccctggtagg
     1801 acgccaccac aatacacaga gcggtcagga accctttcca gccacccgca aagaaaccgc
     1861 catgctctgt gagattgcta aaaccaatcg actgcccgcc attgccaaag ccaaagaaaa
     1921 tcacgcccag gccaatgaca atcatcacga taatcgtggt gactttgatc atcgcgaacc
     1981 agaactcgat ttcgccgtac aaccgcaccg ccgccagatt cgccaacgcc accagcgcca
     2041 ccgcgatcaa tgcgggtatc cactgcgcca tctccgggaa ccaaaactgg acataaacgc
     2101 caatggcggt gatttctgag atccccaccg ccatccacat aaaccagtaa gaccaggcgg
     2161 tgagatagcc aaaaaacggg ctcatataac gatgcgcata aacggcgaac gaaccggtaa
     2221 ccggttcgag gaacaacatt tcgcccattg aacgcatgat gaaaaaaacg aacagcccgg
     2281 cgatgatata ggccaacaat acggatggcc cggcccattt cagggtactg gcggccccca
     2341 taaacaggcc gacgccaatg gtgcccccca gggcgatgag ttcgatatgt cgagcttcca
     2401 gcccacgctg tagctctggt ttgttatctg ccataaatcc tcgtgttgtg tttgcatgct
     2461 ttccggtgtt accggttatc gttatgggta catcgagtgt tgcgaatgtt ttcgtaattc
     2521 aggagaaatg gcaaataaag cattaaaaat ttgaatgctt tgtgtaataa aaaagcagac
     2581 aggcgacgaa gtgaccactc cgtcgcttta caaagagagg aaaatcagag gttgccggtg
     2641 tagtgccagc gtaaataacg cagcaaacga agctgacgct taatgcggct cggctgcgaa
     2701 agcaggcggt agagccactc cagtcccagc gtttgccaga ttttcggtgc gcgttttacg
     2761 tgaccggtga aaacatcgta agtcccgcca acgcccatat acagcgcatc tggatgcacc
     2821 agacggcagt cgcgcatgaa gatctcctgc tttggcgatc ccatcgcaac ggtgacgatt
     2881 tgcgctccgc tggcatgaat gcgttcaaac agcgcctgac gctgctcggg tttaaaataa
     2941 ccatcctgac tgccaacgat attcacattc cattggttgc gcagtttagc ttcagtttgc
     3001 gccagcactt caggtttacc gcccacaaga aataccggcg tcccttcttt gcctgcgcgc
     3061 gccatcagct cttcccagag atcggcaccg gcaacgcggg aaacctgcgc ctgcgggtac
     3121 tttttacgta ctgaacgtac aacgctgatg ccatccgcat atttaaattc ggcagcgtta
     3181 attaactccc tgacctcggc gttatcttca atggtcagca ttttttcggc attaatggca
     3241 accagcgttc cctgcttaag ctgcccgtca gcaaacagat aatcgagggc atgctgcata
     3301 tcacgccaac caatcaactg taagccacgc agcgtatagg tcggtgccgt ggtgttgtta
     3361 ttcattgtta tccttcaacc tgcgtccgga gcgatgattt tgtacgttta tgaatgagtc
     3421 cggcgctttc aaaaagccag tacaacagtt ttgcgatcat cagacatgcg ccgaagacca
     3481 cgataaaaaa gactacgcgt gagacaaacg aatccagccc ttcacgtgcc agcacgatca
     3541 tattgaagat cgccccaaag cagaaactgt gcaatatcgc agccttatag cgattagtct
     3601 cgcggttgcc cagctcatac agccagtcga accatttgat gatcagtcca accacaatcg
     3661 ccccgagtgg gatgaacaac gcgccgccca tcaccaccag tgagcctata agcgtaggcg
     3721 agatcgccag tccggagtgg ttattcagta cttcccaggt aaagtaattg gctgagttca
     3781 gcaccatact cgggcgcccc ggccacagcc aggaagggat aaagacatag aaatcgcgga
     3841 caattggagc caggccctgg aagtcgatgt tgtcgtagtt ctgcaacagc aacgccagat
     3901 tctcccacgg tgaaaaggtg tcgcgagtga gatagagaaa cgtatagaac gcttcatcgc
     3961 cgctcacatt cattccatag cgttttaacg ccagccagaa catgccaaca atccccagca
     4021 cgcccgccgc cgccagcatc cacaacgaaa tccagccgcg aataatgcca ataaacagga
     4081 agatagcgaa tgcgatgatg atattggcgc gagtgccgcc gacaatcata taagtcagca
     4141 agccaaaggc gaccgtgctg acgaggaaaa acagccacgc tttgctgtcc tggcgcagaa
     4201 agtagaccac cagcatcgcc gggatgaaaa agtaaaagaa gcgttttaac gccacgccgg
     4261 agacttcact ggaaaagatc tgactgtagg agttaagccg gaacagcaaa aagccgttgt
     4321 gcatgaagaa gatgccgacg cttaccagcg cgatacccat caggatcacc cacgtaagat
     4381 tggtctccac gcggttcatg gtaaacagcg gacggcgcgg tacatcagca acgcgtttgc
     4441 gtaggcgggt tttgtaggtg acatagtaaa ccgcgtagaa gcagcccgca gaaagcaacg
     4501 cctgcaacaa gatttctggc ggcgcgacac caacatcaaa gcgaaatacc agcacgctgg
     4561 tcagcgggaa gccgaagaaa aaggtgagca aaaacagcaa tgaaaagaag acattgaagt
     4621 taaagcgcac acggcgaaac tcaaaccagg tcagcgtggc aataaacagc gtgcagagca
     4681 gccagaccac aaacaggcca ctgaattcca gcagactcat gcgacctccc cggcggcaat
     4741 cgccaacgcc cgctgccagc cttgtagata gttagggcta aagaaggcaa tggtgttttt
     4801 atccgccgac gccaactggc gctgcgcttc acgcacaata tcctcgttga gatcgtcggt
     4861 agtaaacagc actggcaaat gctgttccgt catatcctgc cagaacggat tttccctgtt
     4921 aagcacacaa ggaatgcccg cctgaatcag taagcaaagc gtaccaatgc cctgctggcg
     4981 ggcaaaaata aagtaaccaa gatcgcactg acgaagtagc gccagatagg cgtcaaattc
     5041 cagtttttcg ctcaaaattt gcagattttc ttcgctgaat aactccagcc ccgcctgacg
     5101 aacttcctca atgtacgctt cgttattagg cggatatccc atcggcacca ccacttttac
     5161 cgtatcgcca aattgctgat gaacggcacg taaagcggcg acatgctcat tgctgcggtc
     5221 gccggagttc cccaccagaa tggtcatttt tccttcacgt tgccgatcgt tcgccatcgt
     5281 attgagagaa gggtccatcc gcgtcgggaa gtacagctgt tctccccgca cctttgggtg
     5341 cgttttggca aaaaagctca aatcgccgcg ggtggcaaat acacagccga ctcgcttttg
     5401 cgccaggcga cgtagtgggt aaaaaagctt atatctcaag ccactggaaa gctcatacag
     5461 gtctgccccc cagatatgcc agaaaaactg gctgggctta atgccaccac tcagcagagc
     5521 cagccacagt gtgggattga actgaccgtg gaaaaaaaat cgctgctgac ggttagcttt
     5581 tgctttcgcg atgaccgctt ccgccagcga ttttttccca gggaaaaatt gcacagaaag
     5641 cgccggacag ctatcactta agccgtcgtc cttgccaaca accataaact cgcgcgcgtg
     5701 ctcgctcgtc gcggccagcg cgtcattgaa aaaccgcaaa acggttcggt tatggtgagg
     5761 gatatccgat cccagtacgt gaatcagtac agtcatgccc gcctacgcca gagtaaaaac
     5821 acgccacaac aaagagaaaa atagacgata taagttgcca tatatgcctg cgccgcgccc
     5881 agtgcaccat gcgcagggat tagccagtgg gcaaatacca tcaataaagt gaactggctg
     5941 acttccgcca gaatataaaa ccgcagtgac gctttggcga tcaccagata accaaagaca
     6001 taagcgccca cttttaacac atcacccact aactgccagg caaagagatc gcgcatagcg
     6061 gtaaatttat tcgacaacag caaccagata gcaaaatcac gcagcaacca gacggtaaaa
     6121 ctcgctgccg ccactgccgg taagacgaat ttcagcgatt taaccacttc ccgggtgata
     6181 tcgcgctttt ccgttagccg cgacaacgtg ggcagcaaat atacgctgaa cgatgccgta
     6241 ataaattgca ggtaggcatc ggaaatactg ctcacccctt gccagatccc cacttcatcc
     6301 cagctatact gcgccgccag cagtttacgc atcatgatgt aagcaacagg caaggtcacc
     6361 gacgtaatca acgccatgag cgtaaatttg ctcaactgcc ctgccagacc gttatcccag
     6421 ctgggtttca gatagcttaa cgggatgaca ccacgtttaa tcaacatgat ggcggcagga
     6481 attaccacca gcgcgggaat cagcgccaga cccagcaacg ccccttcata accgcccaaa
     6541 cggtaactga cgtagtacgc gagaacgcca atcaagctgc cgacaatcag cgataacgca
     6601 ttacctgcgg catcgcgaaa gcctttcatc agcgccagta acaggttgcc ccaggcgatc
     6661 cccatttgca ccagcgccac taaacgcacc agcccctgat agtcggtatt accaaacaat
     6721 ccctggctga ttggcgcagc tgccagcaca aaaaccagcg ccatcagcgt agagaaacca
     6781 agtaccatcg ctgatgaagt gccgaccacg cggcgcagct gttgcggatt atcatggtac
     6841 tgggcaacgt atttggttac accgttaaag atgccagccc cggcaagcac gccgagcacg
     6901 gtaatcaact ggcggaaatt tgccgccagc ccaagccccg ccggaccaaa tgacaccgcc
     6961 agcaacttac cgaccagtaa cccggcacca atcttgacca gtgtactggc cgccgtccac
     7021 aaggacgctt ttgccaacga catatcagga gaagtagttc aacaaagtcg caattaccgt
     7081 acgctgattg acgggcgaca ggttgtagaa cagcggcagg cgcagcaggc gctcgctctc
     7141 tttggtggtg tagcgatctt caccgtggaa ctcaccaaag tgttcccccg cagggcaacc
     7201 gtgcagcgga atgtaatgaa acaccgccat gatttccgct tctttcagaa agttaatcaa
     7261 cgcgctccgg tcatcaatat cccgcagttt aatgtagaac atatgcgcgt tctgcacgca
     7321 gccatcggga atcgacggca gctcgatacg cccggctttc gccagaggcg ctaacgcatc
     7381 gtagtagttt tgccacagcg ccagacgttg ctggttgata cgatccgctg cttccagttg
     7441 cgcccacagg tacgcagctt gcagatcgga catcaaatag ctggagccaa tatcgcgcca
     7501 ggtatattta tcgacctgac cacggaagaa ctggctgcgg ttagtgccct tttcacggat
     7561 gatctcggct cgttcgatta acgctttatc gttaatcagc gtcgcgccgc cttcaccacc
     7621 cgccgtgtag tttttggttt catggaagct aaagcagcca atatgaccaa tggttcccag
     7681 tgcacgccct ttgtaagtgg acatcacgcc ctgagcggca tcttccacca caaacaaatt
     7741 atgctttttc gccaacgcca taatggtgtc catttcgcag gccacacccg cgtaatggac
     7801 cggcacgata acgcgcgttt tgtcggtgat cgctgcttca atcaacgttt cgtcgatgtt
     7861 catggtgtcc gggcgaacat ccacaaagac gattttcgcg ccacgcagca caaaggcatt
     7921 ggcggtggag acaaaggtgt agctcggcat gatcacttca tcgccaggct ggatatcgag
     7981 cagcagcgcc gccatctcca gcgaagcggt gcaggacggc gtcagtaaca ctttggcgct
     8041 gccaaaacgt tgctccagcc actgctggca gcgacgggta aaaccgccat cgccacacag
     8101 tttgccgcta cccattgccg actgcatata gtcgagttcg gttcccacca ccggcggtgc
     8161 gttaaatgga atcatgtgat cacctgtata accagtacgc ggtgctttct acattcgcac
     8221 cactttgtat gtatcgttta agcgcggcgg tgttgcccat ttgggtcgcc acccgcaaag
     8281 ttgttttacc gcgagcatac gcccagttta gcgccgtttg catcagctca gcacctgcac
     8341 cgcgtccagc cagcaggcca attcgcgcat ctgtcgcatt gagttcccgt aaagagacat
     8401 aaccgcgaat atcgccggac gccgcacgta gaatcagaca ttgatgatca aaggtgccgc
     8461 gcacggcatt ttcaatccac tgtgcataaa agcgaccgct ggcgtcaggc gcataccacg
     8521 gcgcacgaaa acggctttgc gcaaatgcgg cgctagctaa ctgacgtaat gcgggaatat
     8581 cggtctcttg tgccacgaca gcaccgctat cactgacatt gttcacgggt agcgccaaat
     8641 caacttcacc ttctaccagg gagaatccca gctgttgcag ggcatccagt tcccccgtat
     8701 ttgatgccgc aattttggcc tgcacccgtg accatggcgc taaggcgtct gacgtcagga
     8761 gcggtgcttc agacgtaatg cgcacgatgg cgctgttaac accaaagaag gcgttttccc
     8821 aggttagtgg ctcaatactg gcgcggacgg gcacgaagta actccagcag atattggccg
     8881 tagccagttt tcgctaatga actggcagca cgcttcacac cctcgtcatc gagccagccg
     8941 ttacgccagg cgatctcttc caggcaggca atcttaaagc cctggcgttt ttctactgtc
     9001 tgcacaaaag tgctggcctc aatcaggctg tcgtgagtgc cggtatccag ccaggcaaat
     9061 ccgcgcccga gcagttcaac ggtcaggttg cccgcctcga ggtacatctg gttgatggag
     9121 gtaatctcca gttcaccgcg ctccgacggc ttcacctgct ttgcgtactc cacgacttta
     9181 ctgtcgtaga aataaagccc ggtcaccgcc cagtttgact tcggctgttt tggcttttct
     9241 tcaagcgaga tagcgcggaa attgtcgtca aattccacca cgccaaagcg ttccgggtcc
     9301 atcacctgat agccaaaaac cgtcgcccct tccgtgcgcg ccgcaacatg acgcagcttc
     9361 ggactgaagc cctgaccgaa gaagatgtta tcgcccagca ccagacaaga aggttcacca
     9421 ttaaggaagg tttcaccgat gataaaggcc tgcgccagac cgtccgggct gggctgttcg
     9481 gcatattcca gctggatacc gaactcacta ccatcgccca gcaggcgctg gaaataacct
     9541 ttatcttccg gcgtagtgat gatgagaatt tcgcggatac cggccagcat cagcaccgac
     9601 agcggatagt aaatcattgg cttatcgtaa atcggcaaca gttgcttcga tacgccgcgc
     9661 gtaatcggat gcaatcgggt accggaaccg cccgccagga taataccttt catgtttgcc
     9721 tccgctgaaa attagccttt cagacctaaa cgctcaccct gatagctgcc gtcctgcacc
     9781 tgcttccacc agctttcatt agccagatac cactgaaccg ttttacgcat tccactttca
     9841 aaggtttcct gcggcagcca gccaagttca cgggcaattt tcgaagcatc aatggcataa
     9901 cgcagatcat gccccggacg gtcagcgaca aaggtgatca ggtcacgata gtgcgccacg
     9961 ccgtgcggct tgttcgaagc cagttcttcc agcagctcgc aaatggtttc cacaacatcg
    10021 agattcttac gctcgttgtg accaccaata ttataggttt caccgacttt cccggtggtc
    10081 gccacgcaat acagcgcgcg ggcgtgatct tccacataca gccagtcacg gatttgctgc
    10141 ccgttgccat ataccggcag cggtttaccc gccagcgcgt tgaggatcat cagcgggatc
    10201 agtttttccg gaaagtggta aggaccgtag ttattcgagc agttggtgat cagcgtcggc
    10261 agaccgtagg tccgcagcca ggcgcgcacc agatggtcgc tgctggcttt tgacgcggaa
    10321 taagggctgc tcggcgcata cggcgtggtt tcggtgaaga aatcatccgt cgagtgcagg
    10381 tcaccatata cttcgtcggt ggagatatga tgaaaacgga acgctgattt tttatcttcc
    10441 gtcagcgtat tccagtaagc ccgcgccgct tcaagcaatg tataagtccc gacaatgttg
    10501 gtttcaataa acgctgccgg gccgtcaata gaacggtcaa catggctttc ggctgccaga
    10561 tgcatgacac agtctggctg atgctcagtg aatacgcgtg ccagttctgc ccgatcgcag
    10621 atatcaactt tctcaaaggc aaagcgctcg ccttgcgcga ccggtgccag cgacatcagg
    10681 tttccggcgt aggtcagctt atcgaccact accaccgcat cgctcgtttc gttgatgata
    10741 taacgcacca gcgccgagcc aataaacccg gcaccacctg ttatcagaat ttttctcatc
    10801 agcgccagac tcctttggca tcgacgacat actgctgatg gacattgtcg ccattgataa
    10861 ctttgaactg actatgatcg accagcatca ccagcacgtc tgccgttgcc agcgcctcgt
    10921 caagctgcgc cagagtacaa agcccggtca gttttttcgg caactggtgg atgttaggct
    10981 caacaaccag cgtttcgccg ctgtgccact gggcgatcag ttcagcgatt tccatcgccg
    11041 ggctttcgcg caggtcatca atattcggtt taaacgccag accaaagcag gcgattttca
    11101 gttcactggc gcgtttatcg gtagcagcaa ggcagtcggc aaccgccgct ttcacctgat
    11161 cgataaccca gaacggtttg tgatcgttaa cttcgcgcgc ggtacggata agccgcgcct
    11221 gctgggggtt ctgtgccacg ataaaccacg gatcaacagc aatgcagtga ccgcccacgc
    11281 cagggccagg ctgaagaata ttaacgcgag ggtgacgatt cgccaggcga atcagttccc
    11341 agacgttaat cccctgatcg gcacaaatca gcgacaattc attagcaaaa gcgatattca
    11401 catcgcggaa gctgttttcg gtgagcttac acatttccgc cgtccgcgag ttagtgacga
    11461 cacactcacc ttcgaggaaa attttgtaca attcgctggc gcgggccgaa caaaccggcg
    11521 tcataccacc aatcacgcga tcgtttttaa tcagctcgac cataacctgg cccggtaata
    11581 cacgttccgg gcagtaagca atgttgacgt ccgcctgctc gcccacctgc tgtgggaaag
    11641 tcagatccgg gcgcatctct gctaaccatt ctgccatctt ctcggttgac cccactggcg
    11701 aggtggattc aaggatcacc agcgcgcctt ttttcagcac tggcgcaatg gagcgagcag
    11761 ccgattcaac gtaggtcata tctggctcat gatcgccttt aaacggcgta ggtacagcaa
    11821 tcagccaggc atccgcttca actggcgtcg tgctcgctcg taaaaaaccg ccttctacgg
    11881 cagtttttac tacgctcgcc aaatcaggct cgacgatatg gatttcgcca cgattgatgg
    11941 tatcaaccgc gtgttggttg atatcgacac caatcacctg tttttgccgt gaggcaaacg
    12001 ctgctgctgt aggcagcccg atgtaaccca gtccgataac agaaatggtc gcaaaactca
    12061 tagtgatatc cgattatttt ttaacgcttc cagaatgcga gagcatgcct gaccatcacc
    12121 atacgggtta tgggcgcggc tcatagcttg atattcgttt tcgtctttta aaagacgcgt
    12181 cacttcctcg acaattcgct gcttatccgt gcctaccaga cgcaccgtac ccgccgtcac
    12241 cgcttccgga cgctcagtgg tatcgcgcat caccagcacc ggtttcccca gcgaaggcgc
    12301 ttcttcctga atgccgcctg agtcagtcaa aatcagccag gcgtggttca tcagccagac
    12361 aaacggtaaa tactcctggg gatcgatcag aatgacattt ttcacatgcc ccagaatgcg
    12421 attgaccggc tctctgacgt tcgggttgag atgcaccgga tagacaatct ggatgtcctg
    12481 gtgcgtggtg gcgatgtctg ccagcgcgtg gcagatttct tcaaagccac gaccgaaact
    12541 ctcacgccta tgaccggtca ccagaatcat ctttttatcg gggtcgataa acgggtaatt
    12601 tgccgccagt tctgaacgca gcgtgtcgct gctcatcacc tggtcacgca cccataacag
    12661 tgcatcaatg actgtattac cggtaatgaa gattcggcta tccgcaacgt tttcacgcag
    12721 caagttttgc cgggaagttt cggttggaga gaagtgatac atcgccagat gcccggtcaa
    12781 tgtacggtta gcctcttccg gccacggcga atagagatcg cccgtgcgca gaccagcctc
    12841 aacgtgacca acaggaatac gctgataaaa cgccgccagg ctggttgcca gcgtcgtcgt
    12901 cgtatcgccg tgaaccagca cgacgtctgg tttgaactcg gcaagaatag gttttagccc
    12961 ttccagaatc cgacaggtta tctctgtcag gccctgtcct ggctgcatta tgttgagatc
    13021 gtagtcaggt acaatggaaa agagtttcag tacctgatca agcatctccc gatgctgcgc
    13081 agtgacgcaa actttagcct caaaaaaagg atcttttgcc aacgcatgca ccaacggagc
    13141 catcttgatg gcttccgggc gcgtaccaaa tacagtcagt actttcacat cgattctctt
    13201 cgaataagcg gcgagcgcct ttgcgctcac cgcagcagtg ttgctatttc gagcaacggc
    13261 gggttaatgc gacaccagcc ccgatcagcc ccccgacaat gccccacata atcatcagga
    13321 aggcacgacg tgggctatcg cgttttaccg gttcttccgg cgtacgcaaa tagcgatagg
    13381 tctgaaaacg cggatccagg gttggaccaa cattcagggt gtttaacatg gctcgattct
    13441 gatcatagtc gagatcaaag gccggaccga cggcctgtaa attttccagt cgagcctgga
    13501 gcattggacg cccaagcagg aacatttctg aatcaggtaa ttcctcggca ggcacatctg
    13561 tcgcactgcg tgaaatatta tgctgctcag caattttcag cgcctgctca atgctgttca
    13621 tccggcggtc gtagatggct ttcgccacct cttcctgacg cttcacctga gctttcatct
    13681 ggatggtgcg tgccgcccat gcgcctttca gctcatcatt cagatggctg gctgcacgct
    13741 ggctggcaaa agcaacatac tgacgtaaca ggttattagc gtcaggcgcg gtttcggcaa
    13801 taagcttcac gctgtcattg accgcgcgag taaagtctcc ggggataaac tggatgttgt
    13861 taatcatttc atccagcaac gccgcatcgg ctttgctgtt gcccaccatc cgctgtttgt
    13921 aatagtcggt ttgcagccag aactctctgc gggtatccca cgaggccagt tgcataacaa
    13981 actctttata ggcttcgtcc atgaccgatg gttgatcggc agaagccatg tttgaacgga
    14041 catccaggtt acgcaaaaat tgctgctgcg agtaatatcc ccccagcata ttcaccgttg
    14101 gacgatcggt aatcgccgtc gagctccact cctgacgagc aaaaaaagta tacgccagcg
    14161 cgattaacgc aaacgccagc cccatgccaa taatccatag cttcccagcc cacaaggtac
    14221 gaaacaaccc acgaatatcc agttcatttt cagcgtcttc ggccggtttc ccaggcattg
    14281 gttgtgtcat cacatcctca tttatttggt taaattgggg ctgccaccac gatttctacg
    14341 cagtctgcgt tttacgcgct taataaagcg agcaactttc caggcacgct taatgcaata
    14401 tccatagagg aagaatgcta gcaaaaagag caccagcatg acccactccg ggacaaaatg
    14461 agaatattct gccagcacgc caatggaagc gagcagtgct gcggcaaggg taatcagcac
    14521 aaacgcctga cgggaagtaa acccggcacg catgatcaaa tggtgaatat gctgacggtc
    14581 aggagagaat gggctcatgc ctttacgcag gcgacggtac ataatcgcca ccatatccat
    14641 tagcggaatg gcgattatcc acaaagcggt aaccgggctg atgggatggg ttttgccctg
    14701 ggtcgtttcg agcaggatcc agataacggt aaaaccaatc agcgtactgc ccgcatcacc
    14761 cataaagact ttgtagcggc gacccaggat accaaggtta agcatgatgt atggcaggat
    14821 ggcggcgatc atcgcaaagc accagattgc gaggctggtt tgcccgtcga accacaaaat
    14881 cataccgatt gctgcaaacg agacgcagga caacccgccc agcaagccat caatgccatc
    14941 aaccatgttg aacgcattaa tggccgccca gacggcaaat agcgtcagga agtaaccaaa
    15001 cggtccgagc accatctccc aggagccaaa gatataaccc aggctactga gataaagttt
    15061 gccgaacacc atcataacaa tgccaacagc ggcctgtatg gtggcacgga tttttacgct
    15121 gatatcaaaa cgatcatcca gcgcgccaat gaaaacaagc acaccggcac aagcgagata
    15181 gagagatgca tgcggaatat agtaatcgac aattccgaac gtgaagcaaa tccctgcgta
    15241 aaccgaaatc cccccaacga gaggtatcaa tccctggtga cgtttgcgga agtttggttt
    15301 atccactaaa ccgacttttt ttgccacctt acgggcaaaa aacagaaaca gtgtcgtgaa
    15361 taaaaaaata ctgatgagat cagtactcac tgtcagtaaa ttcacaatgc atgctctcag
    15421 agaaaattat tagcagaagt ataaccacga agacctttat tcagaaggga aagtccgttc
    15481 cgaacccacc agctctgtgc aaaaattaac catttgatat gcaaattgca taacatgcca
    15541 gcaaattcca ggaattttcc taacatcgcc agcgcggcat taagattaca gatataaaag
    15601 caaaacgcca cgtaaacacg tggcgttttt ggcataagac aaatttatga gcgtttcatc
    15661 atttcgaaga aatcgtcatt ggtcttggtc atggccagtt tattaatgag gaattccatt
    15721 gcatcgattt cgcccatcgg gtgaatgatt ttgcgcagga tccacatttt ctgcagttct
    15781 tcctgagtcg tgagcagctc ttctttacgg gtaccggaac ggttgtagtc gatagccggg
    15841 aagacgcgtt tttcagcgat cttacgagag aggtgcagtt ccatgttgcc tgtaccttta
    15901 aactcttcgt aaataacttc gtccatttta gaaccggtat cgataagcgc cgtcgcgata
    15961 atggtcaggc tgccgccctc ttccacgtta cgcgccgcac caaagaagcg tttcggacga
    16021 tgaagggcat tggcgtccac accaccggtc aacactttac ctgacgccgg aacaacagtg
    16081 ttgtaagcgc gcgccagacg agtgatggag tcgagcagaa tgataacgtc tttcttgtgc
    16141 tcaaccaggc gtttggcctt ctcgatcacc atttccgcaa cctgaacgtg gcgagatgcg
    16201 ggttcgtcaa aggtagaagc aacaacttca ccttttacca gacgctgcat ctcggttact
    16261 tcttccggac gttcgtcgat cagcagaacc atcagcacac aatccgggtg gttgtaagca
    16321 atgctctgag caatgttctg cagcagcata gttttaccgg ctttcggcgg tgccacaatc
    16381 agaccacgct gaccacgacc gataggtgat gccagatcca gtacgcgagc agttaaatct
    16441 tcagtagaac cgttaccacg ttccatacgc agacgagagt ttgcgtgcag cggggttaag
    16501 ttctcaaaga ggattttgtt gcgggcgttt tcaggtttgt cgaagttaac ttcgttaact
    16561 ttcagcagcg caaaatagcg ttcaccttct ttcggcgggc gaatcttacc agagatggta
    16621 tcaccagtgc ggaggttgaa acggcggatt tggctagggg aaacgtagat gtcatcagga
    16681 ccggcgaggt aggagctgtc tgcggaacgg aggaaaccaa atccatcctg caatatctcc
    16741 agtacgccat caccaaagat atcttcgcca ctctttgcgt gctgcttcag gatggcaaaa
    16801 ataatgtcct gcttacgcat gcgagccagg ttttccagcc ccatattttc gccgagagtg
    16861 atcagctcag aaaccggcgt attctttaat tcggtaagat tcatagtggt gtgagttctt
    16921 aaacttgggg taaatctcga acttaatgtt gtgaatggta tggcaggatc atccatgcct
    16981 gtttacggtc atcgactcat gtcttttcgc tgcctggtca caggagagta cgcagaactg
    17041 aaacgacaag acggattgag tgacgagcca gaaatctgtt cacttcgcat ttaagataaa
    17101 acgggaagcg ttggaaatta caagattcaa acttaataag gtatgtttaa tacgaagtca
    17161 acactaagtt agcatgactc acgccgggcg tccagttttt agcgacgggg cacccgaaca
    17221 tgaaattccc ttacgccagg ttagcgtcga ggaactcttt caactgacct ttagacagtg
    17281 cgcccacttt ggttgccgcc acttcaccgt ttttgaacag cagcagagtc gggataccac
    17341 ggatgccata tttcggcgca gtgccagggt tttgatcgat gttcagtttt gcaacggtca
    17401 gtttgccctg atattcgtca gcgatttcat ccagaatcgg ggcgatcatt ttgcacggac
    17461 cgcaccactc tgcccagaaa tcgacgagga tcgccccgtc cgctttgagt acatccgtgt
    17521 caaaactgtc gtcagtcagg tgaataattt tatcgctcat atataactcc acaggaataa
    17581 gcctggcgtg ttggtttcgt tgttggtgta acattaacca actaaaggtt gactttattt
    17641 caccggatac gctttcgtaa agcaatagta agctgatatt ctaccacact atgagcaaaa
    17701 cacatttaac agaacagaag ttttccgact tcgccctgca tccgaaggtt gtagaagccc
    17761 ttgaaaaaaa agggtttcat aactgtacgc ccattcaggc actggccctt ccgctgacgc
    17821 tggcgggtcg tgacgtagcc gggcaggcgc aaaccggtac cgggaaaacg atggcgtttc
    17881 ttacgtcaac gtttcattat cttctctctc atcctgcgat tgccgatcgc aaggtgaatc
    17941 aaccgcgtgc cttaattatg gcaccgacgc gtgaacttgc cgtgcagatc cacgccgacg
    18001 cggaaccgct ggcggaagct accggcctga agctgggcct ggcttacggt ggtgatggct
    18061 atgacaaaca gctgaaagtg ctggaaagcg gcgttgacat tctgattggc accacgggtc
    18121 gtttaattga ctacgccaag cagaaccaca ttaacctcgg tgccattcag gtagtggtac
    18181 tggacgaagc cgatcgcatg tacgatctag gctttattaa agatatccgc tggctgttcc
    18241 gccgtatgcc gcctgcaaac cagcgcctca acatgctgtt ctccgccacg ctttcgtacc
    18301 gggtacgtga actggcgttc gagcagatga acaatgccga atatattgaa gtggaaccgg
    18361 aacagaaaac gggccaccgt attaaagaag agcttttcta cccttctaac gaagaaaaaa
    18421 tgcgtttgct gcaaacgctg atcgaagaag agtggccaga cagagcgatt attttcgcca
    18481 ataccaaaca ccgttgtgaa gagatctggg gccacctggc ggcagatggt catcgtgtcg
    18541 gtttgttgac aggcgatgtc gcgcagaaaa aacgtctgcg tattcttgat gaatttaccc
    18601 gtggcgatct ggatattctg gttgccaccg acgttgccgc gcgtggtttg catattccgg
    18661 cagtgacgca cgtctttaac tacgatttac ccgatgactg tgaagattac gttcaccgta
    18721 ttggtcgtac aggtcgcgca ggcgcaagcg gtcactctat cagcctggcg tgtgaagagt
    18781 atgcattgaa tttgcctgct attgagacct atattggtca ctcaattccg gtaagcaaat
    18841 acaatccgga cgcattgatg accgatctgc caaaaccgct gcgcctcacg cgcccgcgca
    18901 caggcaatgg tccgcgtcgt actggcgctc cgcgtaatcg tcgtcgttca ggttaaataa
    18961 aatgtcatac tcaaaatcat tcaaactgta tcaagatggc aagagaatga atcccgatga
    19021 gcttactgta gtaagtgatt cgggtgaaag aatgcagcca acacagagac agattgaagg
    19081 atgaagagta tgggttccac ctcgtcgctg tatgcagcca ttgatctcgg ttcgaatagt
    19141 tttcatatgc tggttgtgcg cgaggtggct ggaagcatcc agacgctgac gcgaattaaa
    19201 cgcaaagtgc gtctggctgc tggcctgaat agcgaaaatg acctctctaa tgaagcaatg
    19261 gagcgcggtt ggcaatgtct gcgcctgttt gctgaacgtc tgcaagatat ccctccctcg
    19321 caaattcgcg ttgtcgctac ggcgacatta cgacttgccg tcaatgcggg taattttatt
    19381 gccaaagcgc aggaaatcct cggttgtccg gtacaggtga tcagcggtga agaggaagcg
    19441 cgtctgattt atcagggtgt tgctcacacc actggtggtg ccgatcagcg cctggtggtg
    19501 gatataggcg gtgccagtac tgaactggta accggcacgg gtgcacaaac cacctcgttg
    19561 ttcagcctgt cgatgggctg cgtcacctgg ctggaacgct attttgccga tcgtaatctg
    19621 gggcaagaaa attttgatgc tgcagaaaaa gcggcacgcg aagtgttacg tccggttgcc
    19681 gatgaattac ggtatcacgg ctggaaagtg tgcgttggtg cttccggcac cgtgcaggcg
    19741 ttacaggaaa tcatgatggc acaggggatg gatgaacgca ttaccctgga aaagttgcag
    19801 caattgaaac agcgagccat tcattgcggt cggctggagg aactggagat tgacgggctg
    19861 acgctggaac gtgcgttagt gttcccgagt ggtctggcga tcctgatcgc catttttacc
    19921 gaactgaata ttcagtgtat gaccctggcg ggcggtgcgc tgcgtgaagg cctggtctac
    19981 ggcatgttgc atctggccgt cgagcaggat attcgcagcc gtacactgcg taatattcaa
    20041 cgccgcttta tgatcgatat tgatcaggca cagcgcgtag ccaaagttgc ggctaacttc
    20101 ttcgatcagg tggaaaacga atggcatctt gaagcaataa gccgcgattt gctcatcagc
    20161 gcctgtcagc ttcatgaaat cggcctgagc gttgacttca aacaagctcc gcaacacgct
    20221 gcttatctgg tgcgtaacct ggatcttccc ggttttaccc ccgcacagaa gaaactgctg
    20281 gcgacgctac tgctcaacca gactaacccg gtcgatctct catcgctgca tcagcaaaat
    20341 gccgtaccac cgcgcgtcgc agaacaactc tgccgtttac tccgcctggc tatcattttt
    20401 gccagccgtc gccgtgacga tctcgtgcca gagatgacat tacaggctaa ccatgaactg
    20461 ttgaccttga cgcttccgca aggttggcta acccaacatc cgctgggtaa agagattatt
    20521 gctcaggaaa accagtggca gagctatgtc cactggccgc tggaagtgca ttaatgagta
    20581 agtgccggat gcgatgctga cgcatcttct ccggccttga ttatttccct cgttttgccg
    20641 ccatcatcgc tttcagattc gccagatggc tttgcccttt ctgcatccgt tcttcggcac
    20701 tgaccacttt gcgctcctgt tcccaaatca gatcatcctg cggcagctcc agcaaaaagc
    20761 ggctcggctc cgggcgcacc agttcgccgt actggcggcg ttctttacac agcgtaaagg
    20821 tcaattcctt ctgggcgcgg gtaatgccga cataggccag ccgccgctcc tcatcgatat
    20881 tatcttcatc gatactgctc tggtgcggca aaaacccttc ttccataccg accatgtaga
    20941 cataaggaaa ctccagccct tttgacgcgt ggagagtcat cagttgcacc tgatccagct
    21001 cttcttcact ctcaccacgc tccatcatgt cgcgtaaagt aaagcgcgtc accacctggg
    21061 tgagcgtcat cggctcatcc agttcgctgc cttccagcat ctccgtcatc cagctaaaca
    21121 gttggttgac gttcttcatg cgcatttcgg cagctttcgg gctgggcgat gtttcgtaca
    21181 gccaggattc ataatccatg ccgtggatca gatcgcgcac cgcggcaatc ggctcccgct
    21241 ccgccagacg ctggatttct gccaaccagt gagtgaagcg ggtcaatgct tcataaccac
    21301 gtccgctaag cgtctgactc aggcccatat caaagctggc ggtaaacatg cttttattgc
    21361 gcgtcatcgc ccactcaccc agctttttca gcgtcgccgg gccaatctct cgcttcggcg
    21421 tgttaacgat acgcagaaat gcgctgtcat cgtccgggtt agtcagcacg cgcagataag
    21481 ccagcaagtc cttgatttca ggacgagaga aaaacgacgt accaccagaa attttgtacg
    21541 ggatgcggtt ttgcatcagg aatttttcaa acacccgcga ctgatggtta ccgcgataaa
    21601 gaatagcgta atctttgtac tgcgttttat tgacgaagtg atgcgcaatc agttcaccag
    21661 tgacgcgctc ggcttcatgt tcttcgttat tcgcgcttaa aacttttagc tctgtgccgt
    21721 aacccagttc ggagaacaga cgcttttcaa agacgtgcgg gttattggcg atcaggatgt
    21781 tcgccgcttt caaaatacgt ccggaagagc ggtagttctg ctcaagctta atcaccttca
    21841 gcgccggaaa atcctggctc agcagcacca ggttttgcgg acgtgcgccg cgccaggagt
    21901 agatcgactg gtcatcgtca cccaccacgg taaagcgcgc gcggctgccc accagcagtt
    21961 tcaccagttc atactggctg gtgttggtgt cctgatattc gtccaccagc aggtagcgaa
    22021 ttttgttctg ccagcgctcg cggacttctt cattgcgttg cagaagtagc gtcggcaata
    22081 aaatcagatc atcgaagtcg agcacattac aggctttcag gtgtgcatca tacagcccat
    22141 aacaatgggc aaaaatacgg tcccgctcgc caatcgcact ggctgccgcc tgggacggtg
    22201 ttttgagatc gttcttccag ttagagatgg tcgaaatcaa ctgttgcagg agaactttgt
    22261 catcttcaat cagcccttca gtcaactctt tgagcaacgc cagctgatcg gtatcgtcaa
    22321 acagcgaaaa gttcgccttc atccccagcg ccgcatactc acgcttgatg atatccagcc
    22381 ccagcgtgtg gaaggttgag atcatcagcc cacgcgcctc tttgcgaccc agcgtctgtc
    22441 cgacacgctc tttcatctcg cgcgccgcct tattggtaaa ggtcaccgcc gcgatatgtc
    22501 gcgcctgata accgcacccg cggatcagat gggcgatttt attggtgata acacgagttt
    22561 taccggaacc cgcacccgcc agcaccaggc aggggccggt aacgaattcg acagcttgtt
    22621 gttggccggg gtttagacgc ataggaaaat cgctcaatga aagtcgggag gggaaaataa
    22681 cagcgtggta gtatagccag cctcatcctt accactcaag gcacgatcat ggcaaaaaca
    22741 gcagcagcac tgcatatcct tgtaaaagaa gagaaactgg ctctggatct tctcgtgcag
    22801 attaagaacg gtgccgattt cggcaagctg gcgaagaaac actccatttg cccatcaggc
    22861 aaacgcggcg gtgatttagg tgaattccgc cagggtcaga tggttccggc gttcgataaa
    22921 gttgtgttct cttgtccggt actggagcca accggcccgc tgcacaccca gttcggttac
    22981 cacatcatta aggtgctgta ccgtaactaa tagcaaggcc ttctccggga gaaggccttg
    23041 aactctgaat cgctattaac ccgcaacagc aatacgtttc atatccgtca tatagccgcg
    23101 cagtttctta cctacttgct caatcgcatg gctgcgaatc gcttcattta catcacgcag
    23161 ctgcgcgtta tctaccgcac cttccggaat agctttaccc aaatcgcccg gttgcagttc
    23221 tgccataaac ggtttcagca gcggcacaca agcgtaagag aacagatagt taccgtactc
    23281 ggcagtatca gagataacca cgttcatttc atacagacgc ttacgggcga tggtgttggc
    23341 aatcagcggc agttcgtgca gtgattcata gtaagcagat tcttcgatga tgccggaatc
    23401 aaccatggtt tcgaacgcca gttcaacgcc cgctttcacc atcgcgatca tcagtacgcc
    23461 tttatcgaag tactcctgct cgccgatttt gccttcatac tgcggcgcgg tttcgaaggc
    23521 ggttttgcag gtctcttcac gccaggtcag cagtttctta tcatcgttgg cccagtccgc
    23581 catcataccg gaggagaatt cgccggagat gatgtcgtcc atatgtttct ggaacagcgg
    23641 tgccatgatc tctttcagct gttcagacaa cgcgtaagca cgcagtttcg ccgggttaga
    23701 gagacggtcc atcatcagtg tgataccgcc ctgcttcagc gcttcggtga tggtttccca
    23761 accgaactga attagttttt ctgcgtatgc cggatcggtg ccctcttcca ccagcttgtc
    23821 gaagcacagc agcgagccag cctgcaacat accgcacagg atggtttgct cacccatcag
    23881 gtcagatttc acttccgcaa cgaaggacga ttccagcacg cccgcacggt gaccaccggt
    23941 tgctgccgcc catgctttag cgatcgccat gccttcgcct ttcggatcgt tttccgggtg
    24001 aacggcaatc agcgtcggta cgccgaatcc acgtttgtac tcttcacgca cttcggtgcc
    24061 agggcatttc ggcgcaacca tgactacggt gatgtcttta cggatctgct cgcccacttc
    24121 gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg tctttcatca gtggctgtac
    24181 ggtgcgcact acatcagagt gctgcttgtc cggcgtcagg ttaaccacca gatccgcctg
    24241 tgggatcagt tcttcgtaag tacccacttt aaaaccattt tcggtcgctt tacgccagga
    24301 cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag gagatatcga gaccagaatc
    24361 acgcatgttc agaccctggt tcagaccttg tgcgccacag ccgacgatga ctactttttt
    24421 accctgaagg tagctcgcgc catcggcgaa ttcatcgcgc cccataaagc gacatttgcc
    24481 cagctgtgcc agctgctgac gcagattcag tgtattgaag tagttagcca tggtgattcc
    24541 tcgtgatgtt gtgcttctta ttgttgcgtt tgcgagattg aattcactat atgacaggaa
    24601 atttattgcg gaaattgata tattcacaac gtcacattgc aatttctgca acgtcaacat
    24661 tgagggctgc gccattggat ttacgcgatc tgaaaacctt cctgcatctg gcggaaagcc
    24721 gccattttgg ccgcagcgcg cgggcgatgc acgttagccc gtccacgctc tcacggcaga
    24781 ttcagcgcct ggaagaagat ctcggccagc cgctgtttgt gcgcgataac cgcacggtga
    24841 cgctgactga agcgggcgaa gaactgcgcg ttttcgccca gcaaacgctg ttgcagtatc
    24901 agcagctgcg ccacaccatc gaccagcaag ggccgtcgct ctatggcgaa ttacatatct
    24961 tctgctcggt gaccgccgct tacagccatc tgccgccgat tctggatcgc ttccgcgcgg
    25021 aacacccgtc ggtggagatc aaactcacta ccggtgatgc ggcagatgca atggaaaaag
    25081 tcgtcacagg tgaagctgat ctggcgattg ccgggaagcc agagactctg cccggcgcgg
    25141 tggcgttttc gatgctggag aatctggcag tggtgttgat tgcacccgca ttgccctgcc
    25201 cggtgcgtaa tcaggtttct gtcgaaaagc cggactggtc aacggtaccg tttatcatgg
    25261 ccgatcaggg gccggtgcgc cgccgcattg agttgtggtt ccgacgcaat aaaatcagta
    25321 acccgatgat ttacgccacg gttggcgggc atgaagcgat ggtatcgatg gtggcgctcg
    25381 gctgtggcgt ggcattgttg ccggaagtgg tgctggaaaa cagccccgaa ccggtgcgta
    25441 accgcgtgat gattttagag cgcagcgatg agaaaacgcc gtttgagctg ggcgtgtgtg
    25501 cgcagaaaaa gcggctacat gagccgctaa ttgaggcatt ctggaagatt ttgccgaacc
    25561 attaacccgc caaaaagaac ctgaacgccg ggttattggt ttcgtcgtgg caatcgtagc
    25621 ccagctcatt cagccgggtt tcgaaatccg gttcatggtc gccaagttcg aacgccgcca
    25681 gtacgcgccc gtagtcggtg ccatggctgc gatagtggaa caaagaaatg ttccagtgcg
    25741 tacccagcgt gttgaggaag cgcagcagcg cgcccggtga ttccgggaat tcgaagctgt
    25801 agaggcgttc ctgcaacgga tgtgacggac gcccgccgac catatagcgc acatgcaact
    25861 tcgccatttc gtcgtcggag agatccacca cgctgtagcc gccgtcgttg agcatctgca
    25921 aaatttcttt gcgctcttcg aggccgcggc tcaggcgcac gccgacaaag atgcaggcgt
    25981 ttttggcatc ggcaaaacgg tagttgaact cggtgaccga tcgcccgcca agcagttggc
    26041 agaatttgag gaagctgcct ttttcttccg gaatggtcac cgccaacaac gcttcacgct
    26101 gttcgcccag ttcgcagcgt tccgagacgt agcgcaggcc gtggaagttc acgttggcac
    26161 cggaaagaat atgcgccagc cgttcgccgc gaatgttgtg ctgggcgata tattttttca
    26221 ttcccgccag cgccagcgcg ccagagggtt ccgccaccgc acgcacatct tcgaataaat
    26281 ccttcatcgc cgcacagatc gcatcgctat cgacggtgat gatgtcgtcg agatactcct
    26341 ggcataaacg gaaggtttcg tcaccgatgc gttttaccgc gacaccttcg gcgaacagtc
    26401 ccacgcgtgg cagatcaacc ggatgacccg catccagcgc tgctttcagg caggcggagt
    26461 cttccgcttc tacggcgatc actttgattt gcggcatcag ttgtttgatc agcaccgcta
    26521 cgcccgccgc cagaccgccg ccgccgactg gcacaaatac gcggtcgaga tgggcgtcct
    26581 gctggagcag ttccagcgcc agcgtgcctt gcccggcaat caccatcgga tggtcgaacg
    26641 gcggcaccca ggtgaacccc tgctgctgtg acagttcgat cgctttagct ttcgcttcgt
    26701 caaagttcgc gccatgaagc agcacttcgc cgccgaatcc acgtaccgca tcgactttga
    26761 tgtcggcggt ggcggttggc ataacgatca gggccttcac gcctaaccgc gcagaagaaa
    26821 acgcgacgcc ctgcgcgtgg ttacccgcag aagcagtgat cacgccgtgc gctttttgtt
    26881 cttccgtcag gcccgccatc atggcgtatg cgccgcgcag cttaaagctg tgcactggct
    26941 ggcgatcttc gcgcttcacc agaatgacgt tatcaagacg cgacgacagt ttttccattt
    27001 tttgtagcgg cgtaacctgc gccgcctcgt aaaccggcgc gcgcagcact gctcttaaat
    27061 attcggcacc ttccggagca ccggacaggg gttgcgagtc agccattatt aaccccccag
    27121 tttcgattta tcgcgcaccg cacctttgtc ggcgctggtt gccaggctgg cgtaggcgcg
    27181 cagggcaaag gaaacctgac gttcacggtt tttcggcgtc caggctttgt caccccgagc
    27241 ttcctgcgct tcacgacgcg ccgccagttc ggcatcgctt acctgtaact gaatgccacg
    27301 gttcgggatg tcgatagcga tcaggtcacc atcttcaatc agaccaatgc tgccgccgct
    27361 tgccgcttcc ggtgagacgt ggccgatgga aagaccagag gtgccgccag agaaacgacc
    27421 gtcggtgatc agcgcacagg ctttaccgag acccattgat ttcaggaagc tggttgggta
    27481 gagcatttcc tgcatgccag ggccgccttt cgggccttca tagcggatca ccaccacatc
    27541 gcccgcgaca actttgccac cgaggatcgc ttctaccgcg tcgtcctggc tttcgtacac
    27601 tttcgccggg ccggtgaatt tgaggatgct gtcatcgacg cccgcggttt tcacgatgca
    27661 gccgttttca gcaaaattac cgtagagcac cgccaggccg ccgtctttgc tgtaggcgtg
    27721 ttccagcgag cggatacagc cgtttgcgcg atcgtcatcg agagaatccc aacggcaatc
    27781 ctgcgagaat gcctgtgtag tacgaatgcc cgcagggcct gcgcggaaca tattttttac
    27841 cgcgtcatcc tgggtcagca taacgtcgta ttgttccaga gtttgcggca acgtcaggcc
    27901 aagtacgttt ttcacatcac ggttcagtaa ccccgcgcga tccagttcac cgagaatacc
    27961 gataacacca ccagcacggt gaacatcttc catatggtat ttttgggtgc tcggcgcaac
    28021 tttgcataac tgcgggactt tacgcgaaag tttgtcgata tcactcatgg tgaagtcgat
    28081 ttccgcttcc tgcgccgccg ccagcaggtg aagtaccgtg ttggtagagc cgcccatcgc
    28141 gatatccagc gtcatggcat tttcaaacgc cgctttgcta gcaatattgc gcggcagtgc
    28201 actttcgtcg ttttgctcgt agtaacgttt ggtcaattca acaatgcgtt taccggcatt
    28261 gaggaacaac tgcttacggt ccgcgtgggt tgccagcagc gagccgttgc ccggctgcga
    28321 caggcccagc gcttcggtca ggcagttcat tgagttagcg gtaaacatcc cggagcagga
    28381 gccgcaggtc ggacacgcgg aacgttcaac ctgatcgctc tgggagtcag aaactttcgg
    28441 gtctgcgccc tggatcatcg catcaaccag atcgagtttg attatctgat cggacagttt
    28501 ggttttcccg gcctccatcg ggccgccgga aacaaagatc accggaatat tcaggcgcag
    28561 ggaagccatc agcatccccg gggtgatttt gtcgcagtta gagatgcaga ccatggcgtc
    28621 ggcgcagtgg gcgttgacca tatactcaac ggaatcagcg atcagttcgc gagatggaag
    28681 tgaataaagc atccccccgt ggcccatggc aatcccatca tccaccgcaa tggtgttgaa
    28741 ctctttggca acgccgccag ccgcttcaat ttgttcggcg accagtttac cgagatcgcg
    28801 cagatggacg tgacccggta caaattgggt gaacgagttc acaaccgcga taatcggctt
    28861 gccaaaatcg gcgtcggtca ttccggtggc gcgccacagc gcacgagcac ccgccatatt
    28921 acgaccatga gtggtggtgg cggaacggta cttaggcata ctttatttac tcccagtgtc
    28981 tgtctcgtaa atgggacggt gcgtgccgtc ccattttttg tatttattga ttaacttgat
    29041 ctaaccagcc ccatttatct tcggtttcgc cagtgaagag gccgaagaag gcttgctgaa
    29101 tgcgtttggt aaccgggcca caacggcctt cgccaatctg aataccgtct acgctgcgca
    29161 ctggcgtgat ttctgccgcc gtaccggaca taaagacttc atccgccagg tacagggatt
    29221 cgcgcgacag cacctgctca cgcacttcaa ttcccagctc tttcgccagt ttgatgatgg
    29281 catcacgggt aatacccggc agcgcggagg aggtgaacgg tggggtgaac agcacaccat
    29341 ctttcacttc aaacaggttt tcgcctgcgc cttcagagat ataaccgttc acatccagcg
    29401 cgataccttc ctgataaccg tggcggcgcg cttcgctacc caccagcagg gaagagagat
    29461 agttaccacc ggcttttgcc gcagttggga tggtgtttgg tgctgcgcgg ttccaggagg
    29521 aaaccatcgc atcgatcccc tgctccagcg cttctgcgcc cagatacgct ccccacggga
    29581 aagcggcgat aatcacatcg gttgagtatc ccgctggcgg gtttactccc atgccaacat
    29641 caccgacgaa gatcagcgga cggatatagg cgctggtgag attgtttttg cggatcacgt
    29701 cacgacaagc ttccatcagc tcatcaatgc tcagtgaaac cgggaagcga tagattttgg
    29761 cggagtcatg cagacgctgc atatgctcac gatggcggaa tacaaccggt cctttgtgtg
    29821 agtcgtagca acggatgcct tcaaaaaccg aggtgccata gtgcagcgcg tgcgacatca
    29881 catgcacctt cgcgtcttcc cagcgaacca tctccccatt gaaccaaatg taatcagctt
    29941 tcttcgtggt catttttata ttccttttgc gctcaggcgc ggatttgttg tgatgtggtt
    30001 gtgctctggc agatggcaac gtgtgcgacg tccaccagtt tatttaactg actaaacagt
    30061 aagtcgaccg accgtgggct ggcaacggtc aattcgatat ttatattttg tgcatcgctg
    30121 gcggcagcca tattcattga gcagacgtgg aaaccacgat gacgcaccac gcgtaaaaca
    30181 cgttctaagg tttccggatt gaagcgagcc gatacattga cctgatgttg catcatgata
    30241 atttctccaa catttctgaa ttactggcac ctggcggcac cagcggccag acgttctcaa
    30301 gttcgtcgat tgagacatga agcaggtatg gcccatcact gttcagcatg gtgtcgagtg
    30361 ccgcttcaac ctggtcttta cgggtgatgt gttggccagg gatgccgaag gcgctggcta
    30421 acatgaggaa atcggggtta tcggtaaggg tggtttcgct gtatcgttcc tgaaaaaaca
    30481 gttgctgcca ttgtcgaacc atccctaacc gttggttatc gagtaagacg attttcaacg
    30541 gtaactgctt gcgttttacg gtgcccagct cttgcacatt catcatgaaa gagccgtcac
    30601 cggagataca gacaacggta tcgttcggtc gcgcgacttg tgcgccaact gccgccggta
    30661 aaccaaaacc catggtacct aagccgctgg aggtgatgaa attttccggg cgagtgtggg
    30721 cgatgtgctg cgcagcccac atctggtgct gccccacatc tgtggtcacg acgcaatccg
    30781 caggtttacg atccgacagt tgttttaaca acaacggagc gtagatagcg tcaccgggat
    30841 ggtcgtaacg ccaggaatgt tcatcacgca gctgcgcgca gtgttgctgc cagtcattga
    30901 tatttaacgg ctgctgtaat gctggtaaca gagcatttaa atcaccttgt aatgccacat
    30961 gtgcctgacg cagcttgttc atttctgccg ggtcgatatc catatggata acactggcgt
    31021 gtggtgcgaa ggtgttcagt ttgccggtca cccggtcatc aaaacgtgca cccacggcga
    31081 tcagcaggtc acactcctgc accgcgaagt ttgccgcttt ggttccgtgc atccccagca
    31141 tgcccagata gtacggataa tctgcttcta ctgcgcccag ccctttcagc gtacaggtgg
    31201 caggcatttt tgtggcagcg agaaattcac gcaaagccgg aactgcctgc gccatcccca
    31261 cgccaccgcc aacgtacagc atcggttttt gcgcttttgc cagcatctgg cgcgcttgct
    31321 cgacttcggc atgtgggaaa gtcacttcgt tttcaacggt ggtgaaccac ggttccagat
    31381 cgccgctggc taactggata tcttttggga tatcgaccag aaccggacca ggacgacctg
    31441 agctggcaac gtcgaatgct tcaaccatga tgcgcggcaa ctcttccaga gactgcacca
    31501 gaaagctgtg cttggtacag gctaacgaca atcccaggac atccacttcc tgaaatgcgt
    31561 cggtgccgat aaacggtgcg gacacctgac cagtgatggc aacgacaggg atggaatcta
    31621 acagtgcgtc cgcaagcccg gttatcaggt tggttgcgcc cggaccagac gtggcgatac
    31681 atacgccagt tttaccggta gcacgggcat aaccgatagc cgccattgcc gcaccctgct
    31741 catgtcggca cagcaagtgc tccacgccgc cgtcatacaa tgcatcgtaa accggcataa
    31801 ttgcgccacc cggataaccg aaaacggtgt tcacaccctg ttcccgcaac gcatgtacca
    31861 cccactgtgc gccattcata gttagttccc cgtcctgaat cttgagaaac agaattttgt
    31921 gctgttattc attgtctgct cctcggttat gtttttaagg tcaaaaaaac ccccggacct
    31981 ttcggtgcgg gggtcttagt tcgttaaggc ttgatctcta agcctttcct cgtccaagtg
    32041 cagccccgca cggtgggata ataatcacca ccacgctaat cacgaccagg ctaatcactc
    32101 gtagaagggc tgtcattttg tcttttcttg catcttgttc gaaggaatgc ctaaagagtt
    32161 accatagatt ttgcgaatag cacaagatat tttttggcct atttttatga tggctttaat
    32221 aatttcaata gaaattgttg ttttttaaaa agaaaatagt gagtgaaaaa attcaatttc
    32281 agtataaatg ttgtcccctt cctcgtagtt catcctttgt ccgctgcatt tttgcgagca
    32341 tcattccacc gccagaacaa ctcaacttca cttgcacaaa taaacaggaa tcagccacta
    32401 aaattcacga attagcgggc tacagcagcg atatcttatg cacataatgg tgactcaagg
    32461 agggtttatg tcactgtcaa ttgttcatac ccgcgcagcc ctggaagtaa atgcgccccc
    32521 aatcactgtt gaggtacata tcagtaaagg tctacccggc ttaacgatgg tgggcttacc
    32581 agaaacaacg gtgaaagaag ctcgcgatcg cgtgcgcagc gccattatca atagcggata
    32641 tgaatatccg gcgaaaaaaa tcaccatcaa ccttgctcca gccgatctgc caaaagaagg
    32701 gggacgatat gatttaccta tcgccattgc gttgctggcg gcctcagaac agcttacagc
    32761 caataagtta gatgaatatg aattagtcgg agaactagcg cttacaggcg ctctgcgtgg
    32821 cgttcccggt gcaatctcca gtgcaactga agctattaag tcgggcagaa aaattatcgt
    32881 cgcgaaagat aacgaagatg aagtggggct aattaacggt gaaggatgcc tgatagccga
    32941 tcatctgcag gctgtctgtg cgtttctgga aggtaagaac actctcgaac gcccgaaacc
    33001 aactgatgca gtatcccggg cgctacaaca tgatctcagt gatgttgtcg gtcaggagca
    33061 aggaaaacga ggactggaaa ttaccgccgc tggtgggcac aaccttttac tgattgggcc
    33121 gccgggaaca ggtaaaacaa tgctcgccag ccgtattaat ggcctgttgc cagatttaag
    33181 caatgaagag gcactggaga gcgctgcgat attaagtctg gtaaatgctg aatcagtaca
    33241 aaaacaatgg cggcagcgcc cgttccgctc acctcatcac agtgcgtcgt taactgcgat
    33301 ggtgggcggt ggtgcaattc cagggccagg tgaaatttcg ttggcgcata acggcgtgct
    33361 ttttcttgat gagctacctg aatttgaacg gcgtacactg gatgccttgc gagagccgat
    33421 tgaatccggg cagatccatc tttcacgcac acgagcaaaa ataacctatc cagcccgttt
    33481 ccagcttgtt gcggcgatga atcccagccc taccggacat tatcagggaa accataaccg
    33541 ctgcacgcca gaacagacat tacgttatct caaccggctc tcggggccct ttctcgaccg
    33601 cttcgatctc tcactggaga tcccattacc accccccggc attttgagta aaacggtagt
    33661 gccgggagaa agcagcgcca ccgttaaaca acgcgtaatg gccgccagag agcgccaatt
    33721 taagcggcag aataaactga atgcctggct ggatagtccg gaaatacgcc aattctgcaa
    33781 acttgagagc aaagatgcga tgtggttgga agaaacgctg atccatctgg ggttatcgat
    33841 tcgtgcctag cagcggttac tgaaagttgc acgaaccatt gctgatattg atcagtctga
    33901 cattatcaca cgtcagcatt tgcaggaggc agttagctat cgtgcgattg accgtttgct
    33961 catccatctg caaaaactac tgacataaaa aaagggcatt tcgccctttt tattaatcgt
    34021 cagaatcggt gtagtcttca gcaccttcaa cctgcggttt accgccggaa agggtgtgaa
    34081 aacgttttgg acgcttaata cgcatcatat acttggacca cacgcgttct gcttctgtca
    34141 ctggctcacg ttcgccacgg catactgcta cgaagagttt ctcttcctcg gtaaccggct
    34201 cgcgtttgcc aagatccaac tcattgaagg cataaccatg acgctcaagc agttgtgcct
    34261 ctttgatggt gaaatcacca tgacgagaga atccacgtgg ataatgttta ttgtcgaaat
    34321 atcgattagt cgtcgtaaag ctttccgcca tcctgcacgc tcctaattct ttgaccgagc
    34381 tagttatggc gcggagtatt agttacgctt gacagagtgt aaaacaaaac atttaaatca
    34441 taacgacaaa taattttgcg gagagcactg tggatacgga attgttaaaa actttcctgg
    34501 aagttagccg aacgcgtcac tttggtcgag cggctgaatc gctctatctg acccagtcag
    34561 cagtgagctt tcgaatcaga caactggaaa atcaactggg tgtgaacctt ttcacccgcc
    34621 acagaaacaa tatccgttta accgctgccg gtgaaaaact actgccttat gcagaaacgc
    34681 tcatgagcac ctggcaggcc gcccgcaagg aggtggcgca tacctcacga cataacgagt
    34741 tttctatcgg tgccagcgcc tcgttgtggg aatgtatgct taatcagtgg ttgggacgct
    34801 tgtatcaaaa tcaggatgcc catacaggct tacagttcga agcgcgaatt gcccaacggc
    34861 agtctctggt aaaacagctg catgaacgcc agcttgatct tcttattacc actgaagcgc
    34921 ccaaaatgga cgaatttagt agtcagttgc tggggtattt cactttagcg ctttatacca
    34981 gtgccccttc taaactaaag ggagatctta actatctgcg actggagtgg gggccagatt
    35041 ttcaacagca tgaggcaggt ttgatcggtg ctgacgaagt tcccattctg acaaccagtt
    35101 ctgctgaact ggcacagcaa cagattgcga tgcttaatgg ttgcacctgg ctacccgtca
    35161 gctgggcgcg taaaaaaggc ggcctgcata ccgttgtcga tagcacaaca ctttcacggc
    35221 cgctttatgc catatggctg caaaatagcg ataaaaatgc gttgattcgc gatcttttga
    35281 aaattaacgt gctggatgaa gtgtattaat ctgaatggct ggcaaggatg ccggtagaag
    35341 gatttacttc ggagaggatt atttcagata aaaaaaaatc cttagctttc gctaaggatg
    35401 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
    35461 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
    35521 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
    35581 caaccaccgg tttta
//
LOCUS       DBKEKI010000030        34262 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.030, whole genome shotgun sequence.
ACCESSION   DBKEKI010000030 DBKEKI010000000
VERSION     DBKEKI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.030"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..333)
                     /locus_tag="ACUQPX_001435"
     CDS             complement(<1..333)
                     /locus_tag="ACUQPX_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HHV9056186.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYLQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            495..551
                     /gene="ysgD"
                     /locus_tag="ACUQPX_001436"
     CDS             495..551
                     /gene="ysgD"
                     /locus_tag="ACUQPX_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HHV9056187.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            703..1653
                     /gene="corA"
                     /locus_tag="ACUQPX_001437"
     CDS             703..1653
                     /gene="corA"
                     /locus_tag="ACUQPX_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HHV9056188.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1694..2074)
                     /locus_tag="ACUQPX_001438"
     CDS             complement(1694..2074)
                     /locus_tag="ACUQPX_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HHV9056189.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     TYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2088..2468)
                     /gene="yigG"
                     /locus_tag="ACUQPX_001439"
     CDS             complement(2088..2468)
                     /gene="yigG"
                     /locus_tag="ACUQPX_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HHV9056190.1"
                     /translation="MLRMFIPTSNGKISRRRYIFSFILINFIFAFLIIFFNDGEAGFL
                     VIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITMIAEDFACSGNGS
                     MIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2563..3453)
                     /gene="rarD"
                     /locus_tag="ACUQPX_001440"
     CDS             complement(2563..3453)
                     /gene="rarD"
                     /locus_tag="ACUQPX_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HHV9056191.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3505..3972)
                     /gene="yigI"
                     /locus_tag="ACUQPX_001441"
     CDS             complement(3505..3972)
                     /gene="yigI"
                     /locus_tag="ACUQPX_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HHV9056192.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASVLDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4137..5006
                     /gene="pldA"
                     /locus_tag="ACUQPX_001442"
     CDS             4137..5006
                     /gene="pldA"
                     /locus_tag="ACUQPX_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HHV9056193.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5139..6968
                     /gene="recQ"
                     /locus_tag="ACUQPX_001443"
     CDS             5139..6968
                     /gene="recQ"
                     /locus_tag="ACUQPX_001443"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HHV9056194.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7032..7652
                     /gene="rhtC"
                     /locus_tag="ACUQPX_001444"
     CDS             7032..7652
                     /gene="rhtC"
                     /locus_tag="ACUQPX_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HHV9056195.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7714..8334)
                     /gene="rhtB"
                     /locus_tag="ACUQPX_001445"
     CDS             complement(7714..8334)
                     /gene="rhtB"
                     /locus_tag="ACUQPX_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HHV9056196.1"
                     /translation="MTLEWWFAYLLTSIILSLSPSSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8445..9467
                     /gene="pldB"
                     /locus_tag="ACUQPX_001446"
     CDS             8445..9467
                     /gene="pldB"
                     /locus_tag="ACUQPX_001446"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HHV9056197.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9475..10275
                     /gene="yigL"
                     /locus_tag="ACUQPX_001447"
     CDS             9475..10275
                     /gene="yigL"
                     /locus_tag="ACUQPX_001447"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HHV9056198.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10351..11250
                     /gene="bioP"
                     /locus_tag="ACUQPX_001448"
     CDS             10351..11250
                     /gene="bioP"
                     /locus_tag="ACUQPX_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HHV9056199.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPSSSQTADDRRRDCALSE"
     gene            complement(11138..12091)
                     /gene="metR"
                     /locus_tag="ACUQPX_001449"
     CDS             complement(11138..12091)
                     /gene="metR"
                     /locus_tag="ACUQPX_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HHV9056200.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSSERPTYDAPTVRPGSPARL"
     gene            12327..14588
                     /gene="metE"
                     /locus_tag="ACUQPX_001450"
     CDS             12327..14588
                     /gene="metE"
                     /locus_tag="ACUQPX_001450"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HHV9056201.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14627..15442)
                     /locus_tag="ACUQPX_001451"
     CDS             complement(14627..15442)
                     /locus_tag="ACUQPX_001451"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HHV9056202.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15704..16465
                     /gene="udp"
                     /locus_tag="ACUQPX_001452"
     CDS             15704..16465
                     /gene="udp"
                     /locus_tag="ACUQPX_001452"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HHV9056203.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16606..18033
                     /gene="rmuC"
                     /locus_tag="ACUQPX_001453"
     CDS             16606..18033
                     /gene="rmuC"
                     /locus_tag="ACUQPX_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HHV9056204.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18128..18883
                     /gene="ubiE"
                     /locus_tag="ACUQPX_001454"
     CDS             18128..18883
                     /gene="ubiE"
                     /locus_tag="ACUQPX_001454"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HHV9056205.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18897..19502
                     /gene="ubiJ"
                     /locus_tag="ACUQPX_001455"
     CDS             18897..19502
                     /gene="ubiJ"
                     /locus_tag="ACUQPX_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HHV9056206.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19499..21139
                     /gene="ubiB"
                     /locus_tag="ACUQPX_001456"
     CDS             19499..21139
                     /gene="ubiB"
                     /locus_tag="ACUQPX_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HHV9056207.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21218..21487
                     /gene="tatA"
                     /locus_tag="ACUQPX_001457"
     CDS             21218..21487
                     /gene="tatA"
                     /locus_tag="ACUQPX_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HHV9056208.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21491..22006
                     /gene="tatB"
                     /locus_tag="ACUQPX_001458"
     CDS             21491..22006
                     /gene="tatB"
                     /locus_tag="ACUQPX_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HHV9056209.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSAEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22009..22785
                     /gene="tatC"
                     /locus_tag="ACUQPX_001459"
     CDS             22009..22785
                     /gene="tatC"
                     /locus_tag="ACUQPX_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HHV9056210.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22827..23609
                     /gene="tatD"
                     /locus_tag="ACUQPX_001460"
     CDS             22827..23609
                     /gene="tatD"
                     /locus_tag="ACUQPX_001460"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HHV9056211.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23606..24094)
                     /gene="rfaH"
                     /locus_tag="ACUQPX_001461"
     CDS             complement(23606..24094)
                     /gene="rfaH"
                     /locus_tag="ACUQPX_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HHV9056212.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24261..25754
                     /gene="ubiD"
                     /locus_tag="ACUQPX_001462"
     CDS             24261..25754
                     /gene="ubiD"
                     /locus_tag="ACUQPX_001462"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HHV9056213.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25800..26501
                     /gene="fre"
                     /locus_tag="ACUQPX_001463"
     CDS             25800..26501
                     /gene="fre"
                     /locus_tag="ACUQPX_001463"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HHV9056214.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="ACUQPX_001464"
     CDS             complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="ACUQPX_001464"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HHV9056215.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWSCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGMGQGIATVFERV"
     gene            complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="ACUQPX_001465"
     CDS             complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="ACUQPX_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HHV9056216.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDPLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30244..31575
                     /gene="pepQ"
                     /locus_tag="ACUQPX_001466"
     CDS             30244..31575
                     /gene="pepQ"
                     /locus_tag="ACUQPX_001466"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HHV9056217.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31575..32189
                     /locus_tag="ACUQPX_001467"
     CDS             31575..32189
                     /locus_tag="ACUQPX_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HHV9056218.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32228..33679
                     /gene="trkH"
                     /locus_tag="ACUQPX_001468"
     CDS             32228..33679
                     /gene="trkH"
                     /locus_tag="ACUQPX_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HHV9056219.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33691..34236
                     /gene="hemG"
                     /locus_tag="ACUQPX_001469"
     CDS             33691..34236
                     /gene="hemG"
                     /locus_tag="ACUQPX_001469"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HHV9056220.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYGRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8042 a         8664 c         9271 g         8285 t
ORIGIN      
        1 cttctgctga ccgttttcga tgtacaaacc gagcggcgca tagctttcat catagatgcc
       61 gccgttcatc gccatctgca cctgaccctg actattaata tccgccagca gagcatgtaa
      121 cgttccccac gcttcgccat tggctttttg caagtacatt ttcacccgct ctgtttgagg
      181 attaacggta tacgcctgta cggtcagcgt cggatctgag agtgcgcaat catcagcggc
      241 aacagcaaac aagggaagta aggtgagggt gagaaaaatc cgtttgagat tcaaggtgat
      301 cattccttta ccaatgagta gccgatgcgc cattataggt cctggatgtg ggattttttt
      361 atcctgttag cgaccttgac gagtaccaaa aagcgcgaag ttcaactatt gttctgtggt
      421 gttctgttgc gtgttgacgg caaaattttg ctggcgtaac atgcgcgcac gatcactcta
      481 agaggacatt cgccttggac acacccagta gatactggct cactatcctg tcatccagga
      541 tcaactccta aggctatccc tttttgctga tagccttagc ggttgtcagc gacctcaatt
      601 tttcccgtcg cgctgagtca ggctgtttaa tggtctgaaa cccaatttgt ttctgtgtgc
      661 ccaccgaact gtccgatatt ttaagcattg ggagtcccgg tcatgctgag cgcatttcaa
      721 ctggaaaata accgactgac ccggctggaa gtcgaagagt cacaacccct tgtaaatgca
      781 gtatggattg atcttgtcga accggacgac gacgagcgac tgcgcgtaca atctgaactt
      841 ggccagagcc tggcaacccg cccggaactg gaagacatcg aagcatcggc acgtttcttt
      901 gaagacgacg acggcctgca tattcactcc ttcttcttct ttgaagatgc ggaagatcac
      961 gccggtaact ccactgtggc atttaccatc cgtgatggtc gtctgtttac tctgcgtgag
     1021 cgtgaactgc ccgcttttcg tctgtatcgt atgcgtgccc gtagccagtc gatggtagac
     1081 ggtaacgcct acgagttgct gctggatctg ttcgaaacca aaatcgaaca gttggcagat
     1141 gaaattgaaa atatctatag cgacctggag cagttgagcc gggtgattat ggaagggcat
     1201 cagggcgatg agtacgacga ggcgctctcc acgctggcgg aactggaaga tatcggctgg
     1261 aaagttcgcc tgtgtctgat ggatacccag cgcgctctca acttcctggt gcgtaaagcg
     1321 cgtttaccgg gtgggcaact ggagcaggcg cgtgaaatcc tgcgcgatat cgaatccctg
     1381 ctgccgcata acgaatccct gttccagaag gtgaacttcc tgatgcaggc ggcaatgggt
     1441 tttatcaaca tcgagcagaa ccgcatcatc aaaattttct cggtggtatc cgtcgtgttc
     1501 ctgccgccaa cgctggttgc ttccagctac gggatgaact ttgagtttat gccagaactg
     1561 aagtggagtt tcggctaccc tggcgcgatt atctttatga tcctcgcggg cctggcaccg
     1621 tatctgtact ttaagcggaa gaactggttg taaaaagaag gcggaggctt agcctggcta
     1681 agcctcctgt tattcaaagg ctccaggtat ttaacccttt tacctctttc tcataaaacc
     1741 atttgttcgt gttaacagca acataggttg ctacggcaat tcccagaatg ttaacgccaa
     1801 ttaatgcacc aacaaataga gatagaatgc caagtaatag aactataatt gcttttttcc
     1861 ataagcccag aacaaataaa tagatccagg aacagaagaa agcaatgaaa ttcatttgaa
     1921 tagttaatcg ttgtcttact tttaatgctt taaatgctgc tttatattct ggtgttgctc
     1981 cccagaatcc aggaaagcca tgttgatcat aaaaattaaa tcggtatttc catttttcac
     2041 ttaatgaatc atcgttcata tattccttac tcataaatac tccataacta ttgttttgat
     2101 gaatcagtag gtgcaagcat tagcatactg aaagtggaaa aataacaaat caaaaaaatc
     2161 atcgaaccat tgcctgaaca ggcaaaatct tcggctatca ttgtgatgat agagatgata
     2221 tatactgcta atgtaccaaa aacataaatt tttatatata tgaaaccact atcacggagt
     2281 cgttggcaat tcatgttgat gacgagataa tggagtgcga tggtagagac tataacaaga
     2341 aaacctgctt ccccatcgtt aaaaaagata ataagaaagg caaaaatgaa atttattaaa
     2401 ataaatgaaa atatatatcg gcgtctggaa atcttaccgt tagatgttgg gataaacata
     2461 cgtaacataa attttacatc cttgtgtgag tctcctgtca gcatggcaat atgcccactc
     2521 tcatacaaga gtcggcatat tttttcagaa tatatttatt ttttatttgg acgttctacg
     2581 ctgcgtataa atcgcgtcca tcacaaaaat tgccagcgcc acccaaataa aggtgaaagt
     2641 caccatctta tcggcacccg gtttttcacc ataaaacgtc acagccagca ggaacatcag
     2701 cgtcgggcca atgtactgaa aaaagcctaa cgttgagaga cgcaggcgcg tggcagcggc
     2761 agtaaaacac aacagcggta cggtagtgac aataccggcg gcgatcagca gtaaattcag
     2821 cgacatcggg ttttgcccca tatggctggt tgaactgtcg gcaatagcaa acaggtaaat
     2881 cgccgccacg ggcagcagcc acatggtttc aattaacatg ccggtttgtg cttctaccgc
     2941 aatcttcttg cgcaccaggc cgtagaaggc aaaactaaat gccagcccca gcgcgataat
     3001 aggtagcgaa ccaaaagtcc acagctggac caacacgcca catatcgcca gaatcaccgc
     3061 cagccattgc atccggcgga atcgctcgcc gaggaaaatc atccccagca caatgttcac
     3121 cagcgggtta ataaagtaac caaggctcgc ttccagcata tggtgattgt tcaccgccca
     3181 gataaacagt agccagttgc caccaatcag cacggcagag actgccagca taaaaatttt
     3241 ctgtggcgtc tgaatcagcg tttttaaata ggaccactgg cggcaaatgc tcatcagcac
     3301 caccataaag aaaaacgacc agatcacgcg atgcgtcagg atctcatcgg cgggcacgta
     3361 gtaaatcagc ttgaagtatg ctggcgcaat accccaaata aaataagcgg caagagcgag
     3421 taatacgccc tgccgcgttt gttttgcatc catcgggaat actcattttt aattggtaac
     3481 agcagtttac ctgcttttat gtcttcaacc taccatatag gtggcggtgg cactggcaat
     3541 ataaagctgt tcttcattgt gtaattcaac gcgggcgacg gcgactttat tgcctgcacg
     3601 caacaggcta ctggtagcag taaaacgctc gcccctgcct gggcgcagat aatcaacgcg
     3661 aagatcaatg gtccccatcc gcgatagccg ctggcgtagt tcatcttcac tgatggtttc
     3721 gtggcgggtt aaggtacttc ccacgcacac cagaccggcg gcgacatcca gcaccgacgc
     3781 aatgactccg ccgtgcaaaa tgctttgcgc ccagttaccc accatcatcg gctgattttt
     3841 aaaggccagc tgtgcgaact ctttttcgta acgctccagt tccatcccca atgcgcggtt
     3901 aaatggcatg tgataaacaa acatctcacc cactaatttc agggcttgtt cagcggtcag
     3961 tacggcagac atatcatcct tacacttcat tggttaatga aatgttgatt ttatgcttct
     4021 ttgttgttgg tttctacttt aggaagggat aactaacggc aatggagtta agtatgtaaa
     4081 atagcccgca gaaaaacatt caccttatca ataattcgtt acggagaaca cgaccgatgc
     4141 ggactctgca gggctggttg ttgccggtgt ttatgttgcc tatggcagta tatgcacaag
     4201 aggcaacggt gaaagaggtg catgacgcgc cagcggtgcg tggcagtatt atcgccaata
     4261 tgctgcagga gcatgacaat ccgttcacgc tctatcctta tgacaccaac tacctcatct
     4321 acacccaaac cagcgatctg aataaagaag cgattgccag ttacgactgg gcggaaaatg
     4381 cgcgtaagga tgaagtaaag tttcagctga gcctggcatt tccgctgtgg cgtgggattt
     4441 taggcccaaa ctcggtgttg ggtgcgtctt atacgcaaaa atcctggtgg caactgtcca
     4501 atagcgaaga gtcttcaccg tttcgtgaaa ccaactacga accgcaattg ttcctcggtt
     4561 ttgccaccga ttaccgtttt gcaggttgga cgctgcgcga tgtggagatg gggtataacc
     4621 acgactctaa cgggcgttca gacccgacct cccgcagctg gaaccgcctt tatactcgcc
     4681 tgatggcaga aaacggtaac tggctggtag aagtgaagcc gtggtatgtg gtgggtaata
     4741 ctgacgataa cccggatatc accaaatata tgggttacta ccagcttaaa atcggctatc
     4801 acctcggtga tgcggtgctc agtgcgaaag gacagtacaa ctggaacacc ggctacggcg
     4861 gcgcggagtt aggcttaagt tacccgatca ccaaacatgt gcgcctttat actcaggttt
     4921 acagcggcta tggcgaatcg ctcatcgact ataacttcaa ccagacccgt gtcggtgtag
     4981 gggttatgct aaacgacttg ttttgatgaa cggttgagag gttggcaaat ctggaatcca
     5041 gcatccagga ttaccctctc agagactaaa agcattgcag tttctcgcgc aggcgctgaa
     5101 aatagcgcct gtttttattt caggcaatcg gggtgaatgt ggcgcaggcg gaagtgttga
     5161 atctggagtc cggagctaaa caggttttac aagaaacctt tggctaccaa cagtttcgcc
     5221 ccggccagga agaaattatc gacactgtgc tttccggtcg cgattgcctg gttgttatgc
     5281 ccactggtgg cggaaaatcc ctttgctatc aaattcctgc cttattgcta aacggcctta
     5341 ccgtggttgt ttcaccgctg atttcgttga tgaaagatca ggtggatcaa ctacaggcca
     5401 acggcgtggc ggcggcgtgc cttaactcga cgcaaacccg cgaacagcaa cttgaagtga
     5461 tgacaggctg ccgcaccggg caaattcgct tactgtatat cgccccggaa cgcctgatgc
     5521 tggataactt tcttgagcat ctggcgcact ggaatccggt gttattagcc gtcgatgaag
     5581 cgcactgtat ctcccaatgg ggccacgatt tccgcccgga atatgccgcg ctcggtcagt
     5641 tgcgccagcg gttcccgacg ctgccgttta tggcgctgac cgccacagcc gacgacacca
     5701 cgcgccagga tatcgtgcgc ctgctggggc tgaacgatcc gctgattcaa atcagcagtt
     5761 ttgaccgtcc gaatattcgc tacatgctga tggagaaatt caaaccgctc gatcagttga
     5821 tgcgctacgt gcaggaacag cgcggtaagt ctggcattat ctactgcaac agccgggcga
     5881 aagtagaaga caccgctgcg cgcctgcaaa gcaagggtat tagcgcggcg gcctatcatg
     5941 ccgggctgga aaataatgtc cgcgccgacg tgcaggagaa attccagcgc gatgatctgc
     6001 aaattgtggt ggcgacggtg gcgttcggca tgggcatcaa taaaccaaac gttcgcttcg
     6061 tggtccactt tgatattccg cgcaatatcg aatcctatta tcaggaaacc ggtcgcgccg
     6121 ggcgtgatgg cctgcctgcg gaagcgatgc tgttttacga tccggctgat atggcgtggc
     6181 tgcgccgttg tctggaagag aagccgcagg ggcagttgca ggatatcgag cgccacaaac
     6241 tcaatgcgat gggcgcgttt gccgaagcgc aaacttgccg tcgtctggta ttgctgaact
     6301 attttggcga agggcgtcag gagccgtgcg ggaactgcga tatctgcctc gatccgccga
     6361 aacagtacga cggttcaacc gatgctcaga ttgccctttc caccattggt cgtgtgaatc
     6421 agcggtttgg gatgggttat gtggtggaag tgattcgtgg tgctaataac cagcgtatcc
     6481 gcgactatgg tcatgacaaa ctgaaagtct atggtatggg ccgtgataaa agccatgaac
     6541 attgggtgag cgtgatccgc cagctgattc atctcggcct ggtgacgcaa aatattgccc
     6601 agcattctgc cctacaactg acagaggccg cgcgcccggt gctgcgcggc gaatcctctt
     6661 tgcaacttgc cgtgccgcgt atcgtggcgc tcaaaccgaa agcgatgcag aaatcgttcg
     6721 gcggcaacta tgatcgcaaa ctgttcgcca aattacgcaa actgcgtaaa tcgattgccg
     6781 atgaaagtaa tgtcccgccg tacgtggtgt ttaacgacgc aaccttgatt gagatggctg
     6841 aacagatgcc gatcaccgcc agcgaaatgc tcagcgttaa cggcgttggg atgcgcaagc
     6901 tggaacgctt tggtaaaccg tttatggcgc tgattcgtgc gcatgtcgat ggcgacgacg
     6961 aagagtagtc agcagcataa aaaagtgcca gtatgaagac tccgtaaacg tttcccccgc
     7021 gagtcaaatg tatgttgatg ttatttctca ccgtcgccat ggtgcacatt gtggcgctta
     7081 tgagccccgg tcccgatttc ttttttgtct ctcagaccgc tgtcagtcgt tcccgtaaag
     7141 aagcgatgat gggcgtgctg ggcattacct gcggcgtaat ggtttgggct gggattgcgt
     7201 tgcttggcct gcatttgatt atcgaaaaaa tggcctggct gcatacgctg attatggtgg
     7261 gcggtggcct gtatctctgc tggatgggtt accagatgct acgcggtgca ctgaaaaaag
     7321 aggctgtatc tgcgcctgca ccacaggttg agctggcgaa aagcgggcgc agtttcctga
     7381 aaggcttact gaccaatctc gccaatccga aagcgattat ctattttggt tcggtattct
     7441 cattgtttgt cggcgataac gttggcacta ccgagcgctg gggcattttc gcgttgatta
     7501 ttgtcgaaac actggcgtgg tttaccgtcg ttgccagtct gtttgccctg ccgcaaatgc
     7561 gccgtggtta tcaacgtctg gcgaagtgga ttgatggttt tgccggggcg ttatttgccg
     7621 gatttggcat tcatttgatt atttcgcggt gatgccagac gcgtcttcag agtaagtcgg
     7681 ataaggcgtt tacgccgcat ccgacattat ttttcacgca tgcctcgccg atgctaacag
     7741 cgctcccacc agcataaaca acgagccgaa aatcttattc agcgccttca tctgctttgg
     7801 tcctttaatc catagagcaa tcctttgagc aagggtggcg taaccgatca tcacaataat
     7861 atcgaccaca atagtggtga cgccgagcac gatatactgc atcagttgcg gctgttgcgg
     7921 catgatgaat tgcggaaata gcgccgccag aaacacaata cttttgggat tggtgagatt
     7981 cacaaaaact gcgcgctgga acaaatgtcg acgcgattga gtagaggcca gcgatttaag
     8041 atcaattgcg ccagcggcgc gccactgctg gattcccagc caaatcaagt aagccgcgcc
     8101 tgcccacttt aacacttcaa acgcaatcac tgagcgggaa aatagcgtcc ccaaccccac
     8161 gccaaccagc acaatatgaa tcgccagtcc ggtctgaagc ccagcaatag acgccaccgc
     8221 gccgcgataa ccgtggttga gcgaggtggt catagtgttg attgcaccag agcttggcga
     8281 caggcttaaa atgatcgatg tcagcaggta ggcaaaccac cattctaagg tcatgatgaa
     8341 ctcccggtgt gtctattttt gtgccacaat acgctactgt cgcagcgttg tgtcaggcac
     8401 gcttaaaaaa acgattttac gtggtttaag aggcagatta cccgatgttt cagcagcaaa
     8461 aagactggga aacaagagaa aacgcgtttg ctgcttttac catgggaccg ctgactgatt
     8521 tctggcgcca gcgtgatgaa gcagagttta ctggtgtgga tgacattccg gtgcgctttg
     8581 tccgttttcg cgcacagcac catgaccggg tggtagtcat ctgcccgggg cgtattgaga
     8641 gctacgtaaa atatgcggaa ctggcctacg acctgttcca tttggggttt gatgtcttaa
     8701 tcatcgacca tcgcgggcag ggacgttccg gtcgcctgtt agccgatccg catctcgggc
     8761 atgttaatcg ctttaatgat tatgttgatg atctggcggc attctggcag caggaggttc
     8821 agcccggtcc gtggcgtaaa cgctatatac tggcacattc gatgggcggt gcgatctcca
     8881 cattatttct gcaacgccat ccaggtgtat gtgacgccat tgcgctaact gcgccaatgt
     8941 ttgggatcgt gattcgtatg ccgtcattta tggcacggca gatcctcaac tgggccgaag
     9001 cgcatccacg tttccgtgat ggctatgcaa taggcaccgg gcgctggcgc gcgttgccgt
     9061 ttgctatcaa cgtactgacc cacagcagac agcgatatcg acgtaactta cgcttctatg
     9121 ctgatgaccc aacgattcgc gtcggtgggc cgacctacca ttgggtacgc gaaagtattc
     9181 tggctggcga acaggtgtta gccggtgcgg gtgatgacgc cacgccaacg cttctcttac
     9241 aagctgaaga ggaacgcgtg gtggataacc gcatgcacga ccgtttttgt gaactccgca
     9301 ccgccgcggg ccatcctgtc gaaggaggac ggccgttggt aataaaaggt gcttaccatg
     9361 agatcctttt tgaaaaggac gcaatgcgct cagtcgcgct ccacgccatc gttgattttt
     9421 tcaacaggca taactcaccc agcggaaacc gctctacaga ggtttaaatt tcttatgtac
     9481 caggttgttg cgtctgattt agatggcacg ttactttctc ccgaccatac gttatcccct
     9541 tacgccaaag aaactctgaa gctgctcacc gcgcgcggca tcaactttgt gtttgcgacc
     9601 ggtcgtcacc acgttgatgt ggggcaaatt cgcgataatc tggagattaa gtcttacatg
     9661 attacctcca atggtgcgcg cgttcacgat ctggatggca atctgatttt tgctcataac
     9721 ctggatcgcg acattgccag cgatctgttt ggcgtagtca acgacaatcc ggacatcatt
     9781 actaacgttt atcgcgacga cgaatggttt atgaatcgcc atcgcccgga agagatgcgc
     9841 ttttttaaag aagcggtgtt caaatatgcg ctgtatgagc cgggattact ggagccggaa
     9901 ggcgtcagca aagtgttttt cacctgcgat tcccatgaac aactgctgcc gctggagcag
     9961 gcgattaacg ctcgctgggg cgatcgcgtc aacgtcagtt tctctacctt aacctgtctg
    10021 gaagtgatgg cgggcggcgt gtcgaaaggc catgcgctgg aagcagtggc gaagaaactg
    10081 ggctacagcc tgaaggattg tattgcgttt ggtgacggga tgaacgacgc cgaaatgctg
    10141 tcgatggcgg ggaaaggctg cattatgggc agtgcgcacc agcgtctgaa agatctgcat
    10201 ccggagctgg aagttattgg tactaatgcc gacgacgcgg tgccgcatta tctgcgtaaa
    10261 ctctatttat cgtaatcgtt ctttatttgg tcagttgtca acctgatact tcgctacaat
    10321 ggatactcgt taatcaaaga gttttccatt gtggcgctac ttatcatcac cacgattctg
    10381 tgggccttct cctttagctt ttatggcgag taccttgcgg ggcacgtcga tagctatttt
    10441 gcggtgctgg tgcgcgttgg cctggcggca ctcgtttttc tgccgtttct gcgtacccgt
    10501 ggcaatagcc tgaaaacggt cggcctgtat atgctggtgg gcgcgatgca gctcggcgtg
    10561 atgtatatgc tgagtttccg cgcttatctc tacctgacgg tctccgaatt gttgctgttc
    10621 accgtactga cgccgctcta catcacgctg atttatgaca tcatgagtaa gcgccgtctg
    10681 cgctggggct atgcttttag cgccttgctg gcggtgattg gtgccgggat tattcgctat
    10741 gatcaggtca ccgaccattt ctggactggc ttgttgctgg tgcaactctc caatatcact
    10801 tttgccatcg gcatggtggg ttacaaacgc ctgatggaaa ctcgcccgat gccgcagcat
    10861 aacgcctttg cgtggttcta tcttggcgcg ttcctggtgg cggtaattgc atggttcttg
    10921 ctgggaaatg cgcagaaaat gccgcaaacc acgttgcaat ggggcattct ggtgtttctt
    10981 ggcgtggtgg cttccgggat tggctacttt atgtggaact acggcgcgac gcaggtggac
    11041 gccggaacgc tgggcattat gaataatatg cacgttccgg cagggctgct ggtaaacctg
    11101 gctatctggc accaacagcc gcactggcca acgtttatta caggcgcgct ggtgatcctg
    11161 gcctcactgt gggtgcatcg taagtgggtc gctccgagct cttcacaaac ggcagatgat
    11221 cgcaggcgtg attgcgcgct gagcgaataa acgcttccgt aactggctga cgctgctcgc
    11281 catcgcgcac ggcggcgtac agtcggctcc acaagccttc gcccagggtt ttggtcacca
    11341 ccagaccctg gcgctcaaaa ctctctacta cccaatgcgg tagcgcggca atacccatcc
    11401 gcgcggcaac catttgaatc aacaataagg tgttatcgac gcttttcagt gacgggctga
    11461 cgcctgccgg ctgaagaaaa tgccgccaga catccagtcg actacgctgc accggataaa
    11521 ttaatagcgt ctcgctggcg agatcttccg gtgtaatacg cgtttttgcc gccagtggat
    11581 ggtcaggtgc taacaccagg cgtacttcat aatcgaacat cggcgaataa tgcaggccac
    11641 tgcgcggcag gatatcggag gtcatcacca gatccagctc gccctgttgc aacgcgggct
    11701 gcgggtcaaa cgtcacgccc gatttaaaat ccatctctac ctgcggccag ttcttatgga
    11761 aattttctaa agcgggtgtc agccactgaa tacagctatg gcactcaatg gcaatgcgca
    11821 gacgcgtctg ctgcggttca ttgcaggctt gcagtgcctg gctgatttgc ggcagcactt
    11881 ggtttgccag ttgcaacagg atttctccct gcggtgtaaa gcgtagcggc tggctcttac
    11941 gcacaaatag ccggaagcca aggcgttgtt ccagatcgct aaactggtga gacagggcgg
    12001 attgcgtctg atgcaacgtc gccgcagcgg ctgcgagcga gccgcagttc cgcaacgctt
    12061 gtagcgtttt caggtgtttt acttcgatca tgaaagtcct tcacttcggc atgaataatt
    12121 tgcgcttgag gaatatacag taaccgccaa ttatggatgt gtaaacatct ggacggctaa
    12181 aatccttcgt cttttaaatt tatggtgcgt tggctgcgtt tctccacccc ggtcacttac
    12241 ttcagtaagc tcccggcgat gaataaactt gccgccttcc ctaaattcaa aatccatagg
    12301 atttacatat aattagagga agaaaaatga caatattgaa tcacaccctc ggtttccctc
    12361 gcgttggcct gcgtcgcgag ctgaaaaaag cgcaagaaag ttattgggcg gggaactcca
    12421 cgcgtgaaga actgctgaca gtagggcgtg aactgcgtgc tcgtcactgg gatcaacaaa
    12481 agcaagcggg tatcgacctg ctgccggtgg gcgattttgc ctggtacgat catgtactga
    12541 ccaccagttt gctgctgggt aatgttccgc cacgtcatca gaacaaagac ggttcggtag
    12601 atatcgacac cctgttccgt attggtcgtg gacgtgcgcc gactggcgaa cctgcggcgg
    12661 cagcggaaat gaccaaatgg tttaacacca actatcacta catggtgccg gagttcgtta
    12721 aaggccaaca gttcaaactg acctggacgc agctgctgga ggaagtggac gaggcgctgg
    12781 cgctgggcca caacgttaag cctgtgctgc tggggccggt tacttacctg tggctgggga
    12841 aagtgaaagg tgaacagttt gaccgcctta gcctgctgaa cgacattctg ccggtttatc
    12901 agcaagtgct ggcagaactg gcgaaacgcg gcatcgagtg ggtacagatt gatgaacccg
    12961 cgctggtact ggaactacca caggcgtggc tggacgcata caaacccgct tacgacgcgc
    13021 tccagggaca ggtgaaactg ctgctgacca cctattttga aggcgtaacg ccaaatctcg
    13081 acacgattac tgcgctgcct gttcaggggc tgcatgtgga ccttgtacat ggtaaagatg
    13141 acgttgttga actgcacaag cgcctgcctt ctgactggct gctgtctgcg ggtctgatca
    13201 atggtcgtaa cgtctggcgc gccgatctta ctgagaaata tgcgcaaatt aaggacattg
    13261 tcggcaaacg tgatttgtgg gtggcatctt cctgctcgtt gctgcacagc cccatcgacc
    13321 tgagcgtgga aacgcgtctt gatgcagaag tgaaaagctg gtttgccttc gccctacaaa
    13381 aatgccatga actggcactg ctgcgcgatg cgctgaacag tggtgacacg gcagctctgg
    13441 cagagtggag cgccccaatt caggcgcgtc gtcactctac tcgtgtacat aatccggcgg
    13501 tagaaaagcg tctggcggcg atcaccgccc aggacagcca gcgtgcgaat gtctatgaag
    13561 tgcgtgctga agcccagcgt gcgcgtttta aactgccagc gtggccgacc accacgattg
    13621 gttccttccc gcaaaccacg gaaattcgta ccctgcgtct ggatttcaaa aagggcaatc
    13681 tcgacgccaa caactaccgc acgggcattg cggaacatat caagcaggcc attgttgagc
    13741 aggaacgttt gggactggat gtgctggtac atggcgaggc cgagcgtaat gacatggtgg
    13801 aatactttgg cgagcacctc gacggatttg tctttacgca aaacggttgg gtacagagct
    13861 acggttcccg ctgcgtgaag ccaccgattg tcattggtga cattagccgc ccggcaccga
    13921 ttaccgtgga gtgggcgaag tatgcgcaat cgctgaccga caaaccggtg aaagggatgc
    13981 tgacggggcc ggtgaccata ctctgctggt cgttcccgcg tgaagatgtc agccgtgaaa
    14041 ccatcgccaa acagattgcg ctggcgctgc gtgatgaagt ggccgatctg gaagccgctg
    14101 gaattggcat catccagatt gacgaaccgg cgctgcgcga aggtttaccg ctgcgtcgta
    14161 gcgactggga tgcgtatctc cagtggggcg tagaggcctt ccgtatcaac gccgccgtgg
    14221 cgaaagatga cacacaaatc cacactcaca tgtgttattg cgagttcaac gacatcatgg
    14281 attcgattgc ggcgctggac gcagacgtca tcaccatcga aacctcgcgt tccgacatgg
    14341 agttgctgga gtcgtttgaa gagtttgatt atccaaatga aatcggtcct ggcgtctatg
    14401 acattcactc gccaaacgta ccgagcgtgg aatggattga agccttgctg aagaaagcgg
    14461 caaaacgcat tccggcagag cgcctgtggg tcaacccgga ctgtggcctg aaaacgcgcg
    14521 gctggccaga aacccgcgcg gcactggcga acatggtgca ggcggcgcag aatttgcgtc
    14581 gggggtaaat ccaaaccggg tggtaatacc acccggtctt ttctcattac agcgacttct
    14641 tcccaccata ctgcttaaac cattccagca tacgctgcca gccatctttc gcagattcgg
    14701 catgatagct cgggcgataa tcagcgttga atgcatgccc ggcatccggg tacacgataa
    14761 tctctgcttt cgcattagca gcccgcagcg cctggcgcat cgtttcaacg ctctcctgcg
    14821 gaatgctgtt atcctgacca ccatataagc cgagaaccgg cgcgttaaga tcggttgcga
    14881 tatcaacagg ttgtttcggt gaattcagcg acttgtcgcc cgtcagtttg ccgtaccacg
    14941 ccactgcggc ttttagctgt ggattatgcg cggcatacag ccaggtgata cgtccacccc
    15001 agcagaatcc ggtgatcatt aaacgatgaa catcgccgcc gttgcgggaa gcccaactgg
    15061 cgacatgatc gagatcggcc agcacctgcg agtcaggcac ttttgctacc agaccgctaa
    15121 gcaacgtggg gatatcggca aaatcattcg gatcgccttc gcggaagtaa agttcaggtg
    15181 cgatagccag atacccctcc agcgccagac ggcgacaaat gtcgcggata tgttcatgca
    15241 cgccaaaaat ttcctgcact acaatgacca ctggcagtgg gccatcgctt tgctttggtc
    15301 tggcatggta agcaggcatg ttatcccctt gtgaagggat agaggtgaag cccgccacaa
    15361 tcgcgtcgtc cggggtctga acgatggtcg aagcgagagg cgatgcagca ggtgcaaatc
    15421 cagattgttg tgttgttgcc atggtattct ccgtaccctt ataaaaatgt tgcgcaatgt
    15481 taactatagt cagcatgcaa caaatcacat tgcctgaatc ggctcatctt ttatgcagtc
    15541 ctgcagaatg aagagtgatt tatgtgatat gaatcacttt tggtgggtaa atttatgcaa
    15601 cgcatttgcg tcatggtgat gagtatcacg aaaaaatgtt aaacccttcg gtaaagtgtc
    15661 tttttgcttc ttctgactaa accgattcac agaggagttg tatatgtcca agtctgatgt
    15721 ttttcatctc ggcctcacta aaaacgattt acaaggggct acgcttgcca tcgtccctgg
    15781 cgacccggat cgtgtggaaa agatcgccgc gctgatggat aagccggtta agctggcatc
    15841 tcaccgcgaa ttcactacct ggcgtgcaga gctggatggt aaacctgtta tcgtctgctc
    15901 taccggtatc ggcggcccgt ctacctctat tgctgttgaa gagctggcac agctgggcat
    15961 tcgcaccttc ctgcgtatcg gtacaacggg cgctattcag ccgcatatta atgtgggtga
    16021 tgtcctggtt accacggcgt ctgtccgtct ggatggcgcg agcctgcact tcgcaccgct
    16081 ggaattcccg gctgtcgctg atttcgaatg tacgactgcg ctggttgaag ctgcgaaatc
    16141 tattggcgcg acaactcacg ttggcgtgac agcttcttct gataccttct acccaggtca
    16201 ggaacgttac gatacttact ctggtcgcgt agttcgtcac tttaaaggtt ctatggaaga
    16261 gtggcaggcg atgggcgtaa tgaactatga aatggaatct gcaactctgc tgaccatgtg
    16321 tgcaagtcag ggcctgcgtg ccggtatggt agcgggtgtt atcgttaacc gcacccagca
    16381 agagatcccg aatgctgaga cgatgaaaca aaccgaaagc catgcggtga aaatcgtggt
    16441 ggaagcggcg cgtcgtctgc tgtaattctc ttctcctgtc tgaaggccga cgcgttcggc
    16501 cttttgtatt tttgcgtagc gcctcgcagg aaatgccttt ccaactggac gtttgtacag
    16561 cacaattcta ttttgtgcgg gtaagttgtt gcgtcaggag gcgttgtgga tttctcaatc
    16621 atggtttacg cagttattgc gttggtgggg gtggcaattg gctggctgtt tgccagttat
    16681 caacatgcgc agcaaaaagc cgagcaatta gctgaacgtg aagagatggt cgcggagtta
    16741 agcgcggcaa aacaacaaat tacccaaagc gagcactggc gtgcagagtg tgaattgctc
    16801 aataacgaag tgcgcagcct gcaaagtatt aacacctctc tggaggccga tctgcgtgaa
    16861 gtaaccacgc ggatggaagc cgcacagcaa catgctgacg ataaaattcg ccagatgatt
    16921 aacagcgagc agcgcctcag tgagcagttt gaaaacctcg ccaaccgtat ttttgagcac
    16981 agcaatcgcc gggttgatga gcaaaaccgt cagagtctga acagcctgtt gtcgccgcta
    17041 cgtgaacaac tggacggttt ccgccgtcag gttcaggaca gcttcggtaa agaagcacaa
    17101 gaacgccata ccctgaccca cgaaattcgc aatctccagc aactcaacgc gcaaatggcc
    17161 caggaagcga tcaacctgac gcgcgcgctg aaaggcgaca ataaaaccca gggcaactgg
    17221 ggcgaggtag tattgacgcg ggtgctggag gcttccggtc tgcgtgaagg gtatgaatat
    17281 gaaacccagg tcagcatcga aaatgacgcc cgctcgcgga tgcagccgga tgtcatcgtg
    17341 cggctgccgc agggaaaaga tgtggtgatc gacgccaaaa tgacgctggt cgcctatgaa
    17401 cgctatttta acgccgaaga cgactacacc cgcgaaagcg cgctacagga acatatcgcg
    17461 tcggtgcgta accatatccg tttgctggga cgcaaagatt atcaacagct gccggggctg
    17521 cgaactctgg attacgtgct gatgtttatt cccgttgaac ccgctttttt actggcgctt
    17581 gaccgccagc cggagctgat caccgaagcg ttgaaaaaca atatcatgct ggttagcccg
    17641 actacgctgc tggtggcgtt gcgtactatt gccaacctgt ggcgttatga gcatcaaagc
    17701 cgtaacgccc agcaaatcgc cgatcgtgcc agcaagctgt acgacaagat gcgtttgttc
    17761 atcgatgaca tgtccgcgat tggtcaaagt ctcgacaaag cgcaggataa ctaccgccag
    17821 gcaatgaaaa aactctcttc aggtcgcgga aatgtgctgg cgcaggcaga agcgtttcgc
    17881 ggtttaggag tagaaattaa acgcgagatt aatccggatt tggctgaaca ggcggtgagc
    17941 caggatgaag agtatcgact tcggtcggtt ccggagcagc cgaatgatga agcttatcaa
    18001 cgcgatgatg aatataatca gcagtcgcgc tagctcatta ggggtagtta agccgggtag
    18061 aaatctaggg catcgacgcc caatctgtta cacttctgga acaatttttt gatgagcagg
    18121 cattgagatg gtggataagt cacaagaaac gacgcacttt ggttttcaga ccgtcgcgaa
    18181 ggaacaaaaa gcggatatgg tcgcccacgt tttccattcc gtggcatcaa aatacgatgt
    18241 catgaatgat ttgatgtcat ttggtattca tcgtttgtgg aagcgattca cgattgattg
    18301 cagcggcgta cgccgtgggc agaccgtgct ggatctggct ggtggcaccg gcgacctgac
    18361 agcgaaattc tcccgcctgg tcggagaaac tggcaaagtg gtccttgctg atatcaatga
    18421 atccatgctc aaaatgggac gcgagaagct gcgtaatatc ggtgtgattg gcaacgttga
    18481 gtatgttcag gcgaacgctg aggcgctgcc gttcccggat aacacctttg attgcatcac
    18541 catttcgttt ggtctgcgta acgtcaccga caaagataaa gcactgcgtt caatgtatcg
    18601 cgtgctgaaa cccggcggcc gcctgctggt gcttgagttc tcgaagccaa ttatcgagcc
    18661 gctgagcaaa gcctatgatg catactcctt ccatgtgctg ccgcgtattg gctcactggt
    18721 cgcgaacgac gccgacagct accgttatct ggcagaatcc atccgtatgc atcccgatca
    18781 ggataccctg aaagccatga tgcaggatgc cggattcgaa agtgtcgact actacaatct
    18841 gacggcaggg gttgtggcgc tgcatcgtgg ttataagttc tgacaggaga ccggaaatgc
    18901 cttttaaacc tttagtgacg gcaggaattg aaagtctgct caacaccttc ctgtatcgct
    18961 cacccgcgct gaaaacagcc cgctcgcgtc tgctgggtaa agtattgcgc gtggaggtaa
    19021 aaggcttttc gacgtcattg attctggtgt tcagcgaacg ccaggttgat gtactgggcg
    19081 aatgggcagg cgatgctgac tgcaccgtta tcgcctacgc cagtgtgttg ccgaaacttc
    19141 gcgatcgcca gcagcttacc gcactgattc gcagtggtga gctggaagtg cagggcgata
    19201 ttcaggtggt gcaaaacttc gttgcgctgg cagatctggc agagttcgac cctgcggaac
    19261 tgctggcccc ttataccggt gatatcgccg ctgaaggaat cagcaaagcc atgcgcggag
    19321 gcgcaaagtt cctgcatcac ggcattaagc gccagcaacg ttatgtggcg gaagccatta
    19381 ctgaagagtg gcgtatggca cccggtccgc ttgaagtggc ctggtttgcg gaagagacgg
    19441 ctgccgtcga gcgtgctgtt gatgccctga ccaaacggct ggaaaaactg gaggctaaat
    19501 gacgccaggt gaagtacggc gcctatattt catcattcgc acttttttaa gctacggact
    19561 tgatgaactg atccccaaaa tgcgtatcac cctgccgcta cggctatggc gatactcatt
    19621 attctggatg ccaaatcggc ataaagacaa acttttaggt gagcgactac gactggccct
    19681 gcaagaactg gggccggttt ggatcaagtt cgggcaaatg ttatcaaccc gccgcgatct
    19741 ttttccaccg catattgccg atcagctggc gttattgcag gacaaagttg ctccgtttga
    19801 tggcaagctg gcgaagcagc agattgaagc tgcaatgggc ggcttgccgg tagaagcgtg
    19861 gtttgacgat tttgaaatca agccgctggc ttctgcttct atcgcccagg ttcataccgc
    19921 gcgattgaaa tcgaatggta aagaggtggt gattaaagtc atccgcccgg atattttgcc
    19981 ggtcattaaa gcggatctga aacttatcta ccgtctggct cgctgggtgc cgcgtttgct
    20041 gccggatggt cgccgtctgc gcccaaccga agtggtgcgc gagtacgaaa agaccttgat
    20101 tgatgaactg aatttgctgc gggaatctgc caacgccatt cagcttcggc gcaattttga
    20161 agacagcccg atgctctaca tcccggaagt ttaccctgac tattgtagtg aagggatgat
    20221 ggtgatggag cgcatttacg gcattccggt gtctgatgtt gcggcgctgg agaaaaacgg
    20281 cactaacatg aaattgttgg cggaacgcgg cgtgcaggtg ttcttcactc aggtctttcg
    20341 cgacagcttt ttccatgccg atatgcaccc tggcaacatc ttcgtaagct atgaacaccc
    20401 ggaaaacccg aaatatatcg gcattgattg cgggattgtt ggctcgctaa acaaagaaga
    20461 taaacgctat ctggcagaaa actttatcgc cttctttaat cgcgactatc gcaaagtggc
    20521 agagctacac gtcgattctg gctgggtgcc accagatacc aacgttgaag agttcgaatt
    20581 tgccattcgt acggtctgtg aacctatctt tgagaaaccg ctggccgaaa tttcgtttgg
    20641 acatgtactg ttaaatctgt ttaatacggc gcgtcgcttc aatatggaag tgcagccgca
    20701 actggtgtta ctccagaaaa ccctgctcta tgtcgaaggg gtaggacgcc agctttatcc
    20761 gcagctcgat ttatggaaaa cagcgaagcc tttcctggag tcgtggatta aagatcaggt
    20821 cggtattcct gccctggtga gagcatttaa agaaaaagcg ccgttctggg tcgaaaaaat
    20881 gccagaactg cctgaactgg tttacgacag tttgcgccag ggcaagtatt tacagcacag
    20941 tgttgataag attgcccgcg agcttcagtc aaatcatgta cgtcagggac aatcgcgtta
    21001 ttttctcgga attggcgcta cgttagtatt aagtggcaca ttcttgttgg tcagccgacc
    21061 tgagtggggg ctgatgcccg gctggttaat ggcaggtggt ctgatcgcct ggtttgtcgg
    21121 ttggcgcaaa acacgctgat tttttcatcg ctcaaggcgg gccgtgtaac gtataatgcg
    21181 gctttgttta atcatcatct accacagagg aacatgtatg ggtggtatca gtatttggca
    21241 gttattgatt attgccgtca tcgttgtact gctttttggc accaaaaagc tcggctccat
    21301 cggttccgat cttggtgcgt cgatcaaagg ctttaaaaaa gcaatgagcg atgatgaacc
    21361 aaagcaggat aaaaccagtc aggatgctga ttttactgcg aaaactatcg ccgataagca
    21421 ggcggatacg aatcaggaac aggctaaaac agaagacgcg aagcgccacg ataaagagca
    21481 ggtgtaatcc gtgtttgata tcggttttag cgaactgcta ttggtgttca tcatcggcct
    21541 cgtcgttctg gggccgcaac gactgcctgt ggcggtaaaa acggtagcgg gctggattcg
    21601 cgcgttgcgt tcactggcga caacggtgca gaacgaactg acccaggagt taaaactcca
    21661 ggagtttcag gacagtctga aaaaggttga aaaggcgagc ctcactaacc tgacgcccga
    21721 actgaaagcg tcgatggatg aactacgcca ggccgcggag tcgatgaagc gttcctacgt
    21781 tgcaaacgat cctgaaaagg cgagcgatga agcgcacacc atccataacc cggtggtgaa
    21841 agataatgaa gctgcgcatg agggcgtaac gcctgccgct gcacaaacgc aggccagttc
    21901 ggcggaacag aagccagaaa ccacgccaga gccggtggta aaacctgctg cggacgctga
    21961 accgaaaacc gctgcacctt ccccttcgtc gagtgataaa ccgtaaacat gtctgtagaa
    22021 gatactcaac cgcttatcac gcatctgatt gagctgcgta agcgtctgct gaactgcatt
    22081 atcgcggtga tcgtgatatt cctgtgtctg gtctatttcg ccaatgacat ctatcacctg
    22141 gtatccgcgc cattgatcaa gcagttgccg caaggttcaa cgatgatcgc caccgacgtg
    22201 gcctcgccgt tctttacgcc gatcaagctg acctttatgg tgtcgctgat tctgtcagcg
    22261 ccggtgattc tctatcaggt gtgggcattt atcgccccag cgctgtataa gcatgaacgt
    22321 cgcctggtgg tgccgctgct ggtttccagc tctctgctgt tttatatcgg catggcattc
    22381 gcctactttg tggtctttcc gctggcattt ggcttccttg ccaataccgc gccggaaggg
    22441 gtgcaggtat ccaccgacat cgccagctat ttaagcttcg ttatggcgct gtttatggcg
    22501 tttggtgtct cctttgaagt gccggtggca attgtgctgc tgtgctggat ggggattacc
    22561 tcgccagaag acttacgcaa aaaacgcccg tatgtgctgg ttggtgcatt cgttgtcggg
    22621 atgttgctga cgccgccgga tgtcttctcg caaacgctat tggcgatccc gatgtactgt
    22681 ctgtttgaaa tcggtgtctt cttctcacgc ttttacgttg gtaaagggcg aaatcgggaa
    22741 gaggaaaacg acgctgaagc agaaagcgaa aaaactgaag aataaattca accgcccgtc
    22801 agggcggttg tcatatggag tacaggatgt ttgatatcgg cgttaatttg accagttcgc
    22861 aatttgcgaa agaccgtgat gatgttgtag cgcgcgcttt tgacgcggga gttaatgggc
    22921 tactcatcac cggcactaac ctgcgtgaaa gccagcaggc gcaaaagctg gcgcgtcagt
    22981 attcgtcctg ttggtcaacg gcgggcgtac atcctcacga cagcagccag tggcaagctg
    23041 cgactgaaga agcgattatt gagctggccg cgcagccaga agtggtggcg attggtgaat
    23101 gtggtctcga ctttaaccgc aacttttcga cgccggaaga gcaggaacgc gcttttgttg
    23161 ctcagctacg cattgccgca gaattaaaca tgccggtatt tatgcactgt cgcgatgccc
    23221 acgagcggtt tatgacattg ctggagccgt ggctggataa actgcctggt gcggttcttc
    23281 attgctttac cggcacacgc gaagagatgc aggcgtgcgt ggcgcgtgga atttatatcg
    23341 gcattaccgg ttgggtttgc gatgaacgac gcgggctgga gctgcgggaa ttgttgccgt
    23401 tgattccggc ggagaaattg ctgatcgaaa ctgatgcgcc gtatctgctc ccgcgcgatc
    23461 tcacgccaaa gccatcatcc cggcgcaacg agccagccca tctgccccat attttgcaac
    23521 gtattgcgca ctggcgtgga gaagatgccg catggctggc tgccaccacg gatgccaatg
    23581 tcaaaacact gtttgggatt gcgttttaga gtttgcggaa ctcggtgttc ttcacactgt
    23641 gcttaatctc tttattaata agattaagca atagcatgga gcgagcctca ccatcgggtt
    23701 cggtgaaaat ggcctgaaag ccttcgaacg cgccttcggt aataatcacc ttatcacccg
    23761 gataaggggg tgccggatcg acaatgtctt tcggtttata taccgatagc tgatgaatca
    23821 ccgccgatgg gactatcgct ggcgacgcgc caaagcgcac aaagtggctg acgccacgcg
    23881 tagcgctgat ggttgtggta tgaatcactt ctggatcaaa ttccacaaac aggtagttag
    23941 ggaataacgg ttcactgact gcggtacgtt taccccgtac gattttttcc agggtgatca
    24001 tcggagcgag gcaattaacc gcctgtctct caaggtgttc ctgggcacgt tgaagttgcc
    24061 cgcgcttgca gtacagtaaa taccaggatt gcataatgac tcttatccgt ttaatcgggg
    24121 cgcaaggata gcaaaagctt tacgctaagt taattatatt ccccggtttg cgttataccg
    24181 tcagagttca cgctaattta acaaatttac agcattgcaa agatgaacgc cgtataatgg
    24241 gcgcagatta agaggctaca atggacgcca tgaaatataa cgatttacgc gacttcctga
    24301 cgctgcttga acagcagggt gagctaaaac gtatcacgct cccggtggat ccgcatctgg
    24361 aaatcactga aattgctgac cgcactctgc gtgctggtgg gcctgcgctg ttgttcgaaa
    24421 accctaaagg ctactcaatg ccggtgctgt gcaacctgtt cggtacgcca aagcgcgtgg
    24481 cgatgggcat ggggcaggaa gatgtttcgg cgctgcgtga agttggtaaa ttattggcgt
    24541 ttctgaaaga gccggagccg ccaaaaggtt tccgcgacct gtttgataaa ctgccgcagt
    24601 ttaagcaggt gttaaacatg ccgacaaagc gactgcgtgg tgcgccctgc caacaaaaaa
    24661 tcgtctctgg cgatgacgtc gatctcaatc gcattcccat tatgacctgc tggccggaag
    24721 atgccgcgcc gctgattacc tgggggctga ccgtaacgcg cggcccgcat aaagagcggc
    24781 agaatctggg catttatcgc cagcagctga ttggtaaaaa caaactgatt atgcgctggc
    24841 tgtcgcatcg cggcggcgcg ctggattatc aggagtggtg tgcggcgcat ccgggcgaac
    24901 gtttcccggt ttctgtggcg ctgggtgccg atcctgccac gattctcggt gcagtcaccc
    24961 ccgttccgga tacgctttca gagtatgcgt ttgccggatt gctacgcggc accaaaaccg
    25021 aagtagtaaa gtgtatttcc aatgacctcg aagtgccagc cagtgccgaa atcgtcctgg
    25081 aagggtatat cgatcctggt gagatggcgc cggaagggcc gtatggtgac cacacaggtt
    25141 actataatga agtcgatagt ttcccggtgt ttaccgtgac gcatattacc cagcgtgaag
    25201 atgcgattta ccattccacc tataccgggc gtccgccaga tgagcccgcg gtactgggcg
    25261 tggcgttgaa cgaagtgttt gtaccgattc tgcaaaaaca gttcccggaa attgtcgatt
    25321 tttacctgcc gccggaaggc tgttcttatc gtctggcggt agtgacgatc aaaaaacagt
    25381 acgccggaca cgcgaagcgc gtcatgatgg gcgtctggtc gttcttacgc cagtttatgt
    25441 acactaaatt tgtgatcgtt tgcgatgatg acgtcaacgc acgcgactgg aacgatgtga
    25501 tttgggcgat taccacccgt atggacccgg cgcgggatac tgttctggta gaaaatacgc
    25561 ctattgatta tctggatttt gcctcgcctg tctccgggct gggttcaaaa atggggctgg
    25621 atgccacgaa taaatggccg ggggaaaccc agcgtgaatg gggacgtccc atcaaaaaag
    25681 atccagatgt tgtcgcgcat attgacgcca tctgggatga actggctatt tttaacaacg
    25741 gtaaaagcgc ctgatgcgcg tttgttttgc cctatttatc gatccgacag agaaagcgca
    25801 tgacaacctt aagctgtaaa gtgacctcgg tagaagctat cacggatacc gtatatcgtg
    25861 tccgcatcgt gccagacgcg gccttttctt ttcgtgctgg tcagtatttg atggtagtga
    25921 tggatgagcg cgacaaacgt ccgttctcaa tggcctcgac gccggatgaa aaagggttta
    25981 tcgagctgca tattggcgct tctgaaatca acctttacgc gaaagcagtc atggaccgca
    26041 tcctcaaaga tcatcaaatc gtggtcgaca ttccccacgg agaagcgtgg ctgcgcgatg
    26101 atgaagagcg tccgatgatt ttgattgcgg gcggcaccgg gttctcttat gcccgctcga
    26161 ttttgctgac agcgttggcg cgtaacccaa accgtgatat caccatttac tggggcgggc
    26221 gtgaagagca gcatctgtat gatctctgcg agcttgaggc gctttcgtta aagcaccctg
    26281 gtctgcaagt ggtgccggtg gttgaacaac cggaagcagg ctggcgtggg cgtactggta
    26341 ccgtgttaac ggcggtattg caggatcatg gtacgctggc agagcatgat atctatattg
    26401 ccggacgttt tgagatggcg aaaattgccc gtgacctgtt ttgcagtgag cgtaatgcgc
    26461 gggaagatcg cctgtttggc gatgcgtttg catttatctg agatataaaa aacccgcccc
    26521 tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca
    26581 tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg
    26641 gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg
    26701 ctcaaacacc gtcgcaatac cctgacccat accgatacac atcgtcgcca gaccaaactg
    26761 aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc
    26821 cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc
    26881 cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc
    26941 aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg
    27001 aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg
    27061 agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc
    27121 tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa
    27181 cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta
    27241 attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt
    27301 aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc
    27361 ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat
    27421 gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat
    27481 cggcacatgg cccatatgct ccacgccgcc aaccagacat gcctgcgcat cgccagtcat
    27541 gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt
    27601 aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa
    27661 accctgctcc agcgtctgtt gcacacaact ccagtaaata tcgtcgaggg ccgccgcttc
    27721 cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg
    27781 cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat
    27841 gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg
    27901 ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac
    27961 ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc
    28021 gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac
    28081 cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac
    28141 ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt
    28201 cggttggctc acttctgcca gcgggtcttc aacggcggcg tcttcttctt tcttcggctt
    28261 acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt
    28321 ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc
    28381 tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata
    28441 tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc
    28501 cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac
    28561 aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc
    28621 gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg
    28681 cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc
    28741 gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg
    28801 tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc
    28861 ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga
    28921 gatcacgcca gccagtttca gaccatcgat cttgccgcgc tcaagctgct tgttcagcag
    28981 tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac
    29041 cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcccag
    29101 cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac
    29161 atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg
    29221 gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc
    29281 aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt gcgcgaccat
    29341 tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttccag
    29401 cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc
    29461 catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag
    29521 cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc
    29581 cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc
    29641 cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc
    29701 gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc
    29761 caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac
    29821 gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga
    29881 acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag
    29941 gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc
    30001 ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt
    30061 ctcctgaatc cgcaaggtga tctggtcgta ccagatgcat cggagtgtgt attttgtgtt
    30121 aaaaatatgc aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac
    30181 gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa
    30241 aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact
    30301 cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc
    30361 aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg
    30421 gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa
    30481 ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc
    30541 tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg
    30601 cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg
    30661 ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca ttactaccgc
    30721 tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat
    30781 ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt
    30841 gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg
    30901 cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag
    30961 atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc
    31021 cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat
    31081 gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc
    31141 cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt
    31201 gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat
    31261 ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac
    31321 ctcgcggcac cggcaaaata tccgtacctg cgctgcaccc gtattctcca gccgggcatg
    31381 gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa
    31441 gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc
    31501 attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat
    31561 ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag
    31621 agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga
    31681 aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt
    31741 gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg
    31801 cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta
    31861 ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag
    31921 cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat
    31981 taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg
    32041 ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg
    32101 cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt
    32161 cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc
    32221 ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc
    32281 agggaccatg attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc
    32341 ttttacccag accttttttg tcgccctcgc cattggctct atgttgtggt ggccgaaccg
    32401 caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac
    32461 cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat
    32521 taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt
    32581 ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg
    32641 cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat
    32701 gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat
    32761 tgcggaaacg gcgaaaaccc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct
    32821 ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat
    32881 cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat
    32941 taacactatc attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc
    33001 actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat
    33061 cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta
    33121 cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac
    33181 cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt
    33241 atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg
    33301 catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc
    33361 cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg
    33421 gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac
    33481 gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc
    33541 agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct
    33601 gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttgctggtgc tctttacccc
    33661 gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg
    33721 gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc
    33781 cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatggccgt
    33841 gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc
    33901 aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg
    33961 gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg
    34021 aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt
    34081 tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg
    34141 gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa
    34201 atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg
    34261 tc
//
LOCUS       DBKEKI010000031        33908 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.031, whole genome shotgun sequence.
ACCESSION   DBKEKI010000031 DBKEKI010000000
VERSION     DBKEKI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.031"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..563
                     /locus_tag="ACUQPX_001470"
     CDS             <1..563
                     /locus_tag="ACUQPX_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HHV9056221.1"
                     /translation="DIIISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMR
                     GLFSSLAELYSFGNDTASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEML
                     GMHNLILLENICMQKNAECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLE
                     IKPEPEQQVLIQSLYDRASGSASATQ"
     gene            complement(623..1114)
                     /gene="yajQ"
                     /locus_tag="ACUQPX_001471"
     CDS             complement(623..1114)
                     /gene="yajQ"
                     /locus_tag="ACUQPX_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HHV9056222.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1282..2193
                     /gene="panE"
                     /locus_tag="ACUQPX_001472"
     CDS             1282..2193
                     /gene="panE"
                     /locus_tag="ACUQPX_001472"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HHV9056223.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2156..2746
                     /gene="yajL"
                     /locus_tag="ACUQPX_001473"
     CDS             2156..2746
                     /gene="yajL"
                     /locus_tag="ACUQPX_001473"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HHV9056224.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(2800..4248)
                     /gene="thiI"
                     /locus_tag="ACUQPX_001474"
     CDS             complement(2800..4248)
                     /gene="thiI"
                     /locus_tag="ACUQPX_001474"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HHV9056225.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4454..4696
                     /gene="xseB"
                     /locus_tag="ACUQPX_001475"
     CDS             4454..4696
                     /gene="xseB"
                     /locus_tag="ACUQPX_001475"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HHV9056226.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4696..5595
                     /gene="ispA"
                     /locus_tag="ACUQPX_001476"
     CDS             4696..5595
                     /gene="ispA"
                     /locus_tag="ACUQPX_001476"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HHV9056227.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            5620..7482
                     /gene="dxs"
                     /locus_tag="ACUQPX_001477"
     CDS             5620..7482
                     /gene="dxs"
                     /locus_tag="ACUQPX_001477"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HHV9056228.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSCKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            7537..8511
                     /gene="yajO"
                     /locus_tag="ACUQPX_001478"
     CDS             7537..8511
                     /gene="yajO"
                     /locus_tag="ACUQPX_001478"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HHV9056229.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(8565..9083)
                     /gene="pgpA"
                     /locus_tag="ACUQPX_001479"
     CDS             complement(8565..9083)
                     /gene="pgpA"
                     /locus_tag="ACUQPX_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HHV9056230.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLDILS"
     gene            complement(9061..10038)
                     /gene="thiL"
                     /locus_tag="ACUQPX_001480"
     CDS             complement(9061..10038)
                     /gene="thiL"
                     /locus_tag="ACUQPX_001480"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HHV9056231.1"
                     /translation="MACGEFSLIARYFDCVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(10116..10535)
                     /gene="nusB"
                     /locus_tag="ACUQPX_001481"
     CDS             complement(10116..10535)
                     /gene="nusB"
                     /locus_tag="ACUQPX_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HHV9056232.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10555..11025)
                     /gene="ribH"
                     /locus_tag="ACUQPX_001482"
     CDS             complement(10555..11025)
                     /gene="ribH"
                     /locus_tag="ACUQPX_001482"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HHV9056233.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLE
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11114..12217)
                     /gene="ribD"
                     /locus_tag="ACUQPX_001483"
     CDS             complement(11114..12217)
                     /gene="ribD"
                     /locus_tag="ACUQPX_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HHV9056234.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12221..12670)
                     /gene="nrdR"
                     /locus_tag="ACUQPX_001484"
     CDS             complement(12221..12670)
                     /gene="nrdR"
                     /locus_tag="ACUQPX_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HHV9056235.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            12821..13351
                     /locus_tag="ACUQPX_001485"
     CDS             12821..13351
                     /locus_tag="ACUQPX_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HHV9056236.1"
                     /translation="MNTNVFRLLLLGSLSACVQQSEVRQMKHSVSTLNQEMTQLNQET
                     VKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLRIQG
                     ESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKGISV
                     DQLGFVRIHDIQPVMQ"
     gene            13650..14534
                     /gene="tsx"
                     /locus_tag="ACUQPX_001486"
     CDS             13650..14534
                     /gene="tsx"
                     /locus_tag="ACUQPX_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HHV9056237.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14711..15058)
                     /gene="yajD"
                     /locus_tag="ACUQPX_001487"
     CDS             complement(14711..15058)
                     /gene="yajD"
                     /locus_tag="ACUQPX_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HHV9056238.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15187..16158)
                     /gene="secF"
                     /locus_tag="ACUQPX_001488"
     CDS             complement(15187..16158)
                     /gene="secF"
                     /locus_tag="ACUQPX_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HHV9056239.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFCKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16169..18016)
                     /gene="secD"
                     /locus_tag="ACUQPX_001489"
     CDS             complement(16169..18016)
                     /gene="secD"
                     /locus_tag="ACUQPX_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HHV9056240.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18044..18376)
                     /gene="yajC"
                     /locus_tag="ACUQPX_001490"
     CDS             complement(18044..18376)
                     /gene="yajC"
                     /locus_tag="ACUQPX_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HHV9056241.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18399..19526)
                     /gene="tgt"
                     /locus_tag="ACUQPX_001491"
     CDS             complement(18399..19526)
                     /gene="tgt"
                     /locus_tag="ACUQPX_001491"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HHV9056242.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19581..20651)
                     /gene="queA"
                     /locus_tag="ACUQPX_001492"
     CDS             complement(19581..20651)
                     /gene="queA"
                     /locus_tag="ACUQPX_001492"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HHV9056243.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            20744..21325
                     /gene="acpH"
                     /locus_tag="ACUQPX_001493"
     CDS             20744..21325
                     /gene="acpH"
                     /locus_tag="ACUQPX_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HHV9056244.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(21330..23147)
                     /gene="malZ"
                     /locus_tag="ACUQPX_001494"
     CDS             complement(21330..23147)
                     /gene="malZ"
                     /locus_tag="ACUQPX_001494"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HHV9056245.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23303..24676)
                     /gene="proY"
                     /locus_tag="ACUQPX_001495"
     CDS             complement(23303..24676)
                     /gene="proY"
                     /locus_tag="ACUQPX_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HHV9056246.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGVGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPHAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(24752..26071)
                     /gene="brnQ"
                     /locus_tag="ACUQPX_001496"
     CDS             complement(24752..26071)
                     /gene="brnQ"
                     /locus_tag="ACUQPX_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HHV9056247.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26478..27773)
                     /gene="phoR"
                     /locus_tag="ACUQPX_001497"
     CDS             complement(26478..27773)
                     /gene="phoR"
                     /locus_tag="ACUQPX_001497"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HHV9056248.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(27831..28520)
                     /gene="phoB"
                     /locus_tag="ACUQPX_001498"
     CDS             complement(27831..28520)
                     /gene="phoB"
                     /locus_tag="ACUQPX_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HHV9056249.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            28710..29912
                     /gene="sbcD"
                     /locus_tag="ACUQPX_001499"
     CDS             28710..29912
                     /gene="sbcD"
                     /locus_tag="ACUQPX_001499"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HHV9056250.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            29909..33052
                     /gene="sbcC"
                     /locus_tag="ACUQPX_001500"
     CDS             29909..33052
                     /gene="sbcC"
                     /locus_tag="ACUQPX_001500"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HHV9056251.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            33178..>33908
                     /locus_tag="ACUQPX_001501"
     CDS             33178..>33908
                     /locus_tag="ACUQPX_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9056252.1"
                     /translation="MKKVILSLALGTFGLVMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FCYVKPYMMFISGFSETAMTFIMMLV"
BASE COUNT         8544 a         9104 c         8550 g         7710 t
ORIGIN      
        1 gggatataat aataagtaat actggtgaaa aatttagtga agtgggtcta accaaatatg
       61 ctcaacatct cgcatctttc gctgaagaat tttatataaa agagcaaaaa tcacattctg
      121 aaagtgaaat gagaggacta ttttcatcac ttgccgaatt atatagtttt ggtaatgata
      181 ctgccagcgc aaaccgagtg ttccatcagc atgtccctgc cttcaataca gatcatatga
      241 ttcaatattt tataaatgcc aaagaatgga acaatgccag ggaattattg ataaaagagg
      301 aaatgttggg tatgcataat ctgatattgt tggagaatat ttgtatgcaa aaaaatgcag
      361 aatgcatggc acacattacc tttacgctta ataaattaac aacacaacca gcaataacaa
      421 aaatagatag cgtgggtaat gagcaacttt atcagattgg caaaatctac cataagttgg
      481 aaattaaacc tgagccagag caacaggttt taattcagtc gctttatgac cgggcatcag
      541 gaagcgcctc tgcaactcaa taaatgcatg gtcaaaaggg ccaccacggt ggccctgaca
      601 atcagtagct atatccagaa agttaatcgc ggaagttttt gaactggaac ggctgaccga
      661 gatcgccacc gcgcaccatc gccatgacag cctgcaaatc atcacgagat ttgccagtca
      721 cgcggatctc atcgccctga atttgcgcct gcactttcag tttgctgtct ttgatcatct
      781 tgacgatttt tttctgagtg gcgctctcaa tgccctgttt cagtttcgct tccacaaacc
      841 aggttttacc gctatgaacg atattttccg gtacatccag tgaactgcct tcaatgccgc
      901 gcttcagcag cttggcacgc agaatatcca gcaactgatt gacctggaag tcggactcgc
      961 tcaacacttt gatggttttg ctggcgtcgt tcagctcaaa tgaggcttca acgttacgga
     1021 agtcaaaacg ggactccact tcgcgactcg cgttatcgac cgcgttacgt gcttcctgaa
     1081 gatcaacttc agagacaata tcgaaagatg gcatcttttc ctctcccttc atttttgatg
     1141 cgaagcataa tacctgcaaa gttaaataac caacagtcct gcaccaggac gtatccctta
     1201 attgtgcata gtcgcaacag catgcacaat taagggatag ccttattgaa cacccggagt
     1261 ggttgcgggt gaggaggaac aatgaaaatt accgtattgg gatgcggtgc cttagggcaa
     1321 ttatggctta cagcactttg taaacagggt catgaagttc agggctggct gcgcgtaccg
     1381 caaccttatt gtagtgtgaa tctggttgag acagatggtt cgatatttaa cgaatcgctg
     1441 accgccaacg atcccgattt tctcgccacc agcgatctgc tcctggtgac gctgaaagca
     1501 tggcaggttt ccgatgccgt caaaagcctc gcatccacac tgcctgtaac tacgccaata
     1561 ctgttaattc acaacggcat gggcaccatc gaagaattgc aaaacattca gcagccatta
     1621 ctgatgggca ccaccaccca tgccgcccgc cgcgacggca atgtcattat tcatgtggca
     1681 aacggaatca cgcatatcgg tccggcacgg caacaggatg gcgattacag ttatctggcg
     1741 gatattttgc aaaccgtgtt gcctgacgtt gcctggcata acaatattcg cgccgagctg
     1801 tggcgcaagc tggccgtcaa ctgcgtgatt aatccactga ctgccatctg gaattgcccg
     1861 aacggtgaat tacgtcatca tccgcaagaa attatgcaaa tatgcgaaga agtcgcggca
     1921 gtgatcgaac gcgaagggca tcatacttca gcggaagatt tgcgtgatta cgtgatgcag
     1981 gtgattgatg ccacagcgga aaatatctcg tcgatgttgc aggatatccg cgcgctgcgc
     2041 cacaccgaaa tcgactatat caatggtttt ctcttacgcc gcgcccgcgc gcatgggatt
     2101 gccgtaccgg aaaacacccg cctgtttgaa atggtaaaaa gaaaggagag tgaatatgag
     2161 cgcatcggca ctggtttgcc tcgcccctgg tagtgaagag actgaagccg tcaccacgat
     2221 cgacctcttg gttcgcggcg gtatcaaagt caccactgcc agcgtcgaca gcgatggcaa
     2281 cctggcaatt acctgctcgc gcggcgtgaa gctgctggcg gatgcgccgc tggtcgaagt
     2341 ggctgatggc gaatatgacg tgatcgtgct gcctggtggc attaaaggca cggagtgttt
     2401 tcgcgatagc actctgctgg ttgaaaccgt taaacagttc caccgttccg ggcgtatcgt
     2461 cgcggctatt tgcgccgcgc cagccaccgt gctggtgccg cacgatatct ttccgattgg
     2521 caatatgacc ggcttcccga cgctgaaaga caaaattccc gctgaacaat ggcaggacaa
     2581 gcgcgtcgtc tgggatgcac gggtaaaatt gctgaccagc caggggccgg gtacagctat
     2641 cgactttggt ctgaaaatta tcgacctgct ggttgggcgc gagaaagccc atgaagtggc
     2701 atcacaactg gtgatggcgg cagggattta taattattac gagtagtgtc ggatgcggca
     2761 aacgtcgcat ctgaccagat gcgacgtaaa aactacaaat tacgggcgat ataccttcac
     2821 attgttaaag ccttgctcgc gcagatagag cgcctgcaga cggctcatca ccccgcgctc
     2881 acaccacagc agccaggttt tgttctggtc gagatcgcca aatttggtgc tcagtttata
     2941 gaacggcaga gaaaccacat caatcccttc gactttcagt ggcttatctt cctgttcatc
     3001 gacagaacgg atatcgagaa tcacgtcgtt cgggccgaag ccgttgacgg tttccacttc
     3061 caccacttcc tgctcggtct gctgggcgat ttcgcggata tcaacgttat tcgcttcctc
     3121 aaccacttta tcgagaatac tgaagtcgaa cttctcttct tccgcttcaa tcttcgattt
     3181 aaccgctttc accgtcgggc ttttggagat cacgccacaa tattccggca tcgtgcgagc
     3241 aaagtcttcg gtgccaatct ggcgagccag gttgatgatg tgctctttgt cgtaagaaat
     3301 cagcggacgc aggatcagcg tgtcagagac gttatcaatc aggcgcaggt tggtcagcgt
     3361 ctgactggac acctggccga gcgcttcgcc ggtgaccagc gcctgtacgc cgtaacgttc
     3421 agccacttta gatgcggcac gcaccatcat acgtttgagg ataacgccca tctgaccgtc
     3481 gtcgattttc tcgagaattt ccccgacgac cggttcgaaa ttaatggcga caaaacgcac
     3541 gcggtgggag ctgccaaagc ggttccacag ataatgcgcc acctgacgca cgccaatttc
     3601 atgcgctgcg ccgccgaggt taaagaagca gtaatgcacg cggcagccgc gacgcatcaa
     3661 catataactg gaaacaccgg agtcgaaacc accggaaatg agcgacagca catcttcctg
     3721 ggtgccaatc gggaaaccac caataccttc gtagcgacct ttaatcagca ggaggcgatc
     3781 gtcttccact tccagatgaa cagttacatc cgggttagtc agcttcacgc gcgcggattc
     3841 aatatgctga tttaaaccgc cgccgacgta acgttccaca tcaatcgagc taaaatcatg
     3901 tttgccacgg cgcttcacgc gtacgcagaa ggttttgcct tccagctgat cgcgatactg
     3961 aaccaacgct ttctcaaaaa tatcgtgcat gtcggtaaac ggcacgtctt cgacttcgag
     4021 aatatggtgg atacccggaa tacgggtcag ggcgtcgcga atagtcagac gctggttttc
     4081 atctttcgcg cgaacttcga tgttatccca gtggcggacg acagcgagcg tctcatcata
     4141 gtgctttaaa acgttacgaa tgttcccggt aaggattttt ataaagcgca agcgcacaga
     4201 ttggcttttg atggtgattt ccgggaacaa tttaatgata aacttcatgg cggcaatggt
     4261 tcgttggcaa gccttaagcg acttgtatag ggaaaaatac agcagcccac acctgcggct
     4321 gcatccaggc gcggaagtat accactaaca tcgctttgct gcgcacatca ccttaccatt
     4381 gcgcgttatt tgctatttgc cctgagtccg ttaccatgac ggggcgaaaa atattgagag
     4441 tcagacattc attatgccga agaaaaatga ggcgcccgcc agctttgaaa aggcgctgag
     4501 cgagctggaa cagattgtaa cccgtctgga aagtggcgac ctgccgctgg aagaggcgct
     4561 gaacgagttc gaacgcggcg tgcagctggc acgtcagggg caggccaaat tacaacaagc
     4621 cgaacagcgc gtacaaattc tgctgtctga caatgaagac gcctctctaa ccccttttac
     4681 accggacaat gagtaatgga ctttccgcag caactcgaag cctgcgttaa gcaggccaac
     4741 caggcgctga gccgttttat cgccccactg ccctttcaga acactcccgt ggtcgaaacc
     4801 atgcagtatg gcgcattatt aggtggtaag cgcctgcgac ctttcctggt ttatgccacc
     4861 ggtcatatgt ttggcgttag cacaaacacg ctggacgcac ccgctgctgc cgtagagtgt
     4921 atccacgctt actcattaat tcatgatgat ttaccggcga tggatgatga cgatctgcgt
     4981 cgcggtttgc cgacctgcca tgtgaagttt ggcgaagcaa acgcgattct cgctggcgac
     5041 gctttacaaa cgctggcgtt ctcgattcta agcgatgcca atatgccgga agtgtcggat
     5101 cgcgacagaa tttcgatgat ttctgaactg gcgagcgcca gcggtattgc cggaatgtgc
     5161 ggtggtcagg cattagattt agacgcggaa ggcaaacacg tacctctgga cgcgcttgag
     5221 cgtattcatc gtcataaaac cggcgcattg attcgcgccg ccgttcgcct tggtgcatta
     5281 agcgccggag ataaagggcg tcgtgctctg ccagtactcg acaagtatgc agagagcatc
     5341 ggccttgcct tccaggttca ggatgacatc ctggatgtgg taggagatac tgcaacgttg
     5401 ggaaaacgcc agggtgccga ccagcaactt ggtaaaagta cctaccctgc acttctgggt
     5461 cttgagcaag cccggaagaa agcccgggat ctgatcgacg atgcccgtca gtcgctgaaa
     5521 caactggctg aacagtcact cgatacctcg gcactggaag cgctagcgga ctacatcatc
     5581 cagcgtaata aataaacaat aagtattaat aggcccctga tgagttttga tattgccaaa
     5641 tacccgaccc tggcactggt cgactccacc caggagttac gactgttgcc gaaagagagt
     5701 ttaccgaaac tctgcgacga actgcgccgc tatttactcg acagcgtgag ccgttccagc
     5761 gggcacttcg cctccgggct gggcacggtc gaactgaccg tggcgctgca ctatgtctac
     5821 aacaccccat ttgaccaatt gatttgggat gtgggacatc aggcttatcc gcataaaatt
     5881 ttgaccggac gccgcgacaa aatcggcacc atccgtcaga aaggcggtct gcacccgttc
     5941 ccgtggcgcg gcgaaagcga atatgacgta ttaagcgtcg ggcattcatc aacctccatc
     6001 agtgccggaa ttggtattgc ggttgctgcc gagaaagaag gcaaaaatcg ccgcaccgtc
     6061 tgtgtgattg gcgacggcgc gattaccgct ggcatggcgt ttgaagcgat gaatcacgcg
     6121 ggcgatattc gtcctgatat gctggtggtc ctcaacgaca atgaaatgtc gatttccgaa
     6181 aatgttggcg cgcttaacaa ccatctggct cagctgcttt ccggtaagct ttactcttca
     6241 ctgcgcgaag gcgggaaaaa agttttctct ggcgtgccgc ccattaaaga gctgctcaaa
     6301 cgtaccgaag aacatattaa aggcatggtg gtgcctggca cgttgtttga agagctggga
     6361 tttaactaca tcggcccggt ggacggtcac gatgtgctgg ggcttatcac cacgctaaag
     6421 aacatgcgcg acctgaaagg cccgcagttc ctgcatatca tgaccaaaaa aggtcgtggt
     6481 tatgaaccgg cagaaaaaga cccgatcact ttccacgccg tgcctaaatt tgatccctcc
     6541 agcggttgtt tgccgaaaag tagcggcggt ttgccgagct attcaaaaat ctttggcgac
     6601 tggttgtgcg aaacggcagc gaaagacaac aagctgatgg cgattactcc ggcgatgcgt
     6661 gaaggttccg gcatggtcga gttttcatgt aaattcccgg atcgttactt cgacgtggct
     6721 attgccgagc aacacgcagt gacctttgcc gccggtctgg cgattggtgg atacaaaccc
     6781 attgtcgcga tctactccac cttcctgcaa cgcgcctatg atcaggtgct acatgacgtg
     6841 gcaattcaaa agctaccggt cctgttcgcc attgaccgcg cgggcattgt tggtgctgac
     6901 ggtcaaactc accagggcgc ttttgatctc tcttacctgc gctgcatacc agaaatggtc
     6961 attatgaccc cgagcgatga aaacgaatgt cgccagatgc tctataccgg ctatcactat
     7021 aacgatggcc cgtccgcggt gcgctacccg cgcggcaacg cggttggcgt ggaactgacg
     7081 ccgctggaaa aactaccaat tggcaaaggc attgtgaagc gtcgtggcga gaaactggtg
     7141 atccttaact ttggtacgct gatgccagaa gcggccaaag tcgccgaatc gctgaacgct
     7201 acgctggtcg atatgcgttt tgtgaaacca cttgatgaag cgttaattct ggaaatggcc
     7261 gccagccatg aagcgctggt caccgtagaa gaaaacgcca ttatgggcgg cgcaggtagc
     7321 ggagtgaacg aagtgctgat ggcccatcgt aaaccagtac ccgtgctgaa cattggcctg
     7381 ccggacttct ttattccgca aggaactcag gaagaaatgc gcgccgaact cggcctcgat
     7441 gccgccggta tggaagccaa aatcaaggcc tggctggcat aatccctact ccactcctgc
     7501 tatgcttaag aaattattca tggcaggagt ggcagcatgc aatacaaccc cttaggaaaa
     7561 accgaccttc gcgtttcccg actttgcctc ggctgtatga cctttggcga gccagatcgc
     7621 ggtaatcacg catggacact gccggaagag agcagccgtc ccataattaa acgcgcgctg
     7681 gaaggtggca taaatttctt tgataccgcc aatagctatt ccgatggtag cagcgaagag
     7741 atcgttggtc gcgcactgcg ggatttcgcc cgtcgtgaag acgtagtcgt tgccaccaaa
     7801 gtgttccatc gcgttggtga tttaccggaa ggattatccc gtgcacaaat tttgcgctct
     7861 atcaacgaca gcctgcgacg tctcggcatg gattatgtcg atatcctgca aattcatcgc
     7921 tgggattaca acacgccgat cgaagagacg ctggaagccc tgaacgacgt ggtaaaagcc
     7981 gggaaagcgc gttatatcgg cgcgtcatcc atgcacgctt cgcagtttgc tcaggcgctg
     8041 gaactacaaa aacagcactg ctgggcgcag tttgtcagta tgcaggatca ctataatctg
     8101 atttatcgcg aagaagagcg cgagatgctg cctctgtgtt atcaggaggg cgtggcggtg
     8161 attccgtgga gcccgctggc gcgggggcga ctgacgcgtc catggggaga aactaccgca
     8221 cgactggtgt ctgatgaggt ggggaaaaat ctctataaag aaagcgatga aaatgacgcg
     8281 cagatcgcag agcggttaac gggcgtcagt gaagaactcg gtgcaacacg agcacaagtt
     8341 gcgctggcct ggttgttaag taaaccgggc attgccgcac cgattatcgg aacttcgcgg
     8401 gaagaacagc ttgatgagct gctgaacgcg gtggatatca ctttgaagcc agaacagatt
     8461 gccgagctgg aaacgccgta taaaccgcat gcggtcgtag gatttaaata agagtccagg
     8521 cctgatgaga cgtgacaagc gtcacatcag gcatcggtgc acaactacga cagaatatcc
     8581 agcggccagt gatgaccgat aaagtacagg atgcctgcgg aaatcacccc ggcgacaata
     8641 tcgtcgatca tgatccccat gccgccatgt acattgcgat caaaccagcg gatcggccac
     8701 ggcttccaca tatccagaat acggaaaatc acaaacccgg cggcaaccca ctgccagtca
     8761 ttggtcggca gcgccatgag cgtgatccac ataccaataa attcgtccca gacaatgctg
     8821 ccgtgatcgt gcacgcccat atctttcgcc gtttgatgac aaagatagac gccgatacag
     8881 atccccaaca tcaccaccag cgagtagagc tgccagggta aaaaggtcat cagataccag
     8941 aaagggatcg ccgccagcga tcccatcgtg ccaggaacga ttgggcttaa tccacttccg
     9001 aatccgacag caagcagatg ccacggatta ctcatcttca ggcgactttt cgcgacatct
     9061 ttatggcgtg gcaaaatggt catatccttt ccagtcaaac gtgacaggtt cgccgtcacg
     9121 aataaaacaa agcccttcga tatcggcggt catttgcccg atacaggtaa acggtacgcc
     9181 aaggtgtccg agagcaacat ccagcgcgcc acggttcagt tccggcaccg tgaaacacaa
     9241 ctcgtaatct tcaccgccag agagcgccca gcgcagcgcc tgttccggtt caacatggcg
     9301 agaaagcgca tcagaaaacg gcagcaatgc caggtcaata cgtgcgccgc agtcgctggc
     9361 tttcacgata tgcccgagat cggaaatcag accgtcagag agatcgatag ctgaatttgc
     9421 cagatcgcgc agtgcctgcc cctgtaaaat acgcggcgat ggacggagat gacgtttgat
     9481 caagtagtca gcatctttag catcggcaac ctgcaaacgg ttttgcaaaa tcgccagccc
     9541 ggcggcgcta tcgcccggtg taccggtcac atagatccag tcacccggtt tcgccccaga
     9601 gcgcgttaag gctcgtccca tcggaacaaa gccgtggata cccaacgtca ttgataatgg
     9661 cccacgcgtg gtatcgccgc caatgagttg catatcgtaa taattgagaa gatcaaacaa
     9721 actgtcgctg aaggactcaa gccacgcttc gtctacgtcc ggtaaggtta atgccagcgt
     9781 cagccaggcc ggatcggccc ccatcgctgc cagatcgctt aggttcaccg ccagtgcttt
     9841 ataagccaga tcagcaggat cgatatcagg gaggaaatgg ttgcccgcca ccagcgtatc
     9901 agtgctgatc gccagggtct gtttctcggg gatattgaga agagcgcaat cgtcgccgat
     9961 gcccagttcg acatcaagac gagaacttct tacacagtca aaataacggg caatcaggga
    10021 gaactcgcca catgccatac gttatgcctc agcaggtaaa aaagaaaagg ccggcgatca
    10081 tggaacagtc ttccgtgaat ctaccggcct ggatattact ttttgttagg gcgaatcaca
    10141 ggtgctgctt tatcgagtac gccgttgacg aacttatggc tgtcttctgc gccgaacgat
    10201 ttcgccagtt cgatcgcttc gttaatggcc actttgtatg gcacatcact acgtttagac
    10261 agttcgtaca gcgcaatgcg cagtactgct ttttctacct ggcccagttc ttccagcagg
    10321 cgggacaggt agggcttcat cagtccgtcg aggtatgcgg tgttagtcgc caccccggcc
    10381 agcagctcac ggaagtacag gacgtcaacg tcttttacat cctgttcagc caggaactgg
    10441 tattcaacat cagcgatgtc gttctgggac aactgccagg agtagagcgc ctggacggca
    10501 cactcacgag cgcggcgacg agcagcaggt ttcacggatt tccccttact aatttcaggc
    10561 cttgatggct ttcaatacat taatcatttc aagcgcggtc agtgcagctt ctgcaccttt
    10621 gttgccagct ttggtgccag cacgttcgat cgcttgttca atgctttcag tggtcagaac
    10681 cccaaaagca accggaattt cgctgtcctg ggcaacatgc tccaggccgt tgcttgcacc
    10741 accagcgaca tattcaaagt gggcagtgcc accacgaata accgtaccca gcgcaatcac
    10801 cgcgtcgtat ttaccggttt tagccagtgc acccgccgcc agcggcagct cataggcacc
    10861 aggcacccaa acaacggtaa tgttttcatc ttttacctga ccgatacgtt tcagtgcgtc
    10921 aattgcacct tccagcaggc tgtcattgat aaagttgttg aaacgcgcga tggtgatggc
    10981 gacgcgagcg tccggggtag caacgttagc ttcaataatg ttcattttct tccttcgggt
    11041 tcgagtatgg ccccgcaggg gggcggattt tagcataata tttcgtgcgc tgcttccctt
    11101 tcgagccggg agatcatgca cccactaaat gcaggcaaac atccgggcct acatgacgta
    11161 tctctttgaa tttaaattgg ggggcgtcgg ctaatttctc aagccctggc agcgagcata
    11221 atccacgggc gtcgctgcct aatagtttag gtgcgatata gacaatcagc tcatcgacta
    11281 aacccgcctg tagcaatacg ccagcgagcg ttggccccgc ttccacccag atgctgttaa
    11341 tctgctgttt acccagttgc atcatcagta caaccagatc cagatgacct ttatgctctg
    11401 gaatcagcaa ggtacgcacc gtttccggcc actcacgaga atcttcctgc gtacgcgcga
    11461 accaggtttc gccgggctgc tgcacaatgc gatgttccgg cgtcacgcga ttttggctat
    11521 caatcacaat acgtactggc tggcggagat tttgttgcgg gtagagcgcc tgagtttgtt
    11581 catccagttc agaccaacgc actgttaagg ctggatcatc cgccagcacc gtggcgctgc
    11641 tggttaaaat ggcatgactt tgtgcgcgct gtcgttgtac gtcgcgccgc gcctgaggcg
    11701 aagtgatcca ctggctttcg ccgctcgcca tcgccgtgcg gccatcaagc gatgcgccaa
    11761 gttttaactg aatataagga aagccggtgc gcatccgctt gagaaagcct ttattcaatt
    11821 gctcggcttc actcatcatc agcccgtggc tgacgtcaat gccagcctgt tgcagacggt
    11881 aaagtccacg cccagcgacc tgcgggttag gatcttgcat cgctgcgacc acgcgcgcta
    11941 cgccagcggc gatgagtgcg tcacagcacg gtggcgtacg gccatgatgg ctacagggtt
    12001 cgagtgtcac atacgccgtt gcgcctttgg ctttttcacc cgccatacgc aacgcgtgta
    12061 cttcggcatg tggttcaccc gctcgttggt ggtaaccttc accgacaatt tcgccatctt
    12121 tgacaatgac gcacccgaca ttcgggttgg ggtgggtggt aaaacgtcct cgttgcgcca
    12181 gctttagcgc ccgcgccatg taatactcgt cctgcacggc ttagtcctcc aggcgcgcga
    12241 tctcttcgcc aaattctttg atatcttcga aactgcggta gacagaggca aaacggatat
    12301 aggcgacttt atcgagcttt ttcaattgct ccatcaccag attgccaatc atcttgctcg
    12361 gtacttcgcg ctcaccggtg gcgcgcagct gcgatttaat atgattgatt gccatttcaa
    12421 cgtaatcgga actcaccgga cgtttttcca gcgcccgcag cattccgcta cgcaatttct
    12481 cttcattaaa cggttcacgc acgtcgttgc tttttacaac acgcggcata accagctccg
    12541 ccacttcaaa ggtggtgaaa cgttcattac acaccagaca ctgccgacgg cggcgtacgg
    12601 atgaaccctc gcccacgaga cgagagtcaa ttaccttagt gtccacggcg aaacagaatg
    12661 ggcaatgcat acggcgtcct gaccaggtgg ttaacggaaa tctattttac cctgaactga
    12721 cgatacaaca aaggcgcaac gcttttgaga taaaggatcg atgctttcgc tgcttttacc
    12781 ctctaccatt aggctaattg cgacgagaaa gggacgacct atgaatacta acgtttttcg
    12841 actgctcctg ctgggaagcc ttagcgcctg tgtgcagcaa agtgaagtgc gacagatgaa
    12901 acacagcgtc agcacgctga accaggagat gacgcaactc aatcaagaaa cagtcaaaat
    12961 cactcagcaa aacaggctga atgcaaaatc cagcagtggg gtttaccttc tgcctggagc
    13021 gaaaacaccg gcaagactgg aaagccagat cggtacttta cgtatgtcgc tggtgaatat
    13081 tacgcctgat acagatggca ccacccttac actacgtatt cagggtgagt cgaatgatcc
    13141 cttgcccgca ttcagcggaa ctgttgaata tgggcaaatt cagggaacaa tagacaactt
    13201 tcaggaaatc aatgtgcaga atcaattgat taatgcccct gccagcgtcc tcgcccccag
    13261 cgatgttgat attccgttac aattaaaggg gatttctgtg gatcagttag gctttgtacg
    13321 tatccacgac attcaacctg ttatgcagta aacgtatttc gggacgattt tgtgcgtccc
    13381 gcaacatctt ttcccgtcat tttgttactc tgcttacatc acctggattg atagtaaaag
    13441 tttgcaacaa gggcgaaagt cagtacaatc cccgcccgaa tgtgtgtaaa cgtgaacgca
    13501 atcgattacg taaatgatag aactgtgaaa cgaaacatat ttttgtgagc aatgattttt
    13561 ataataggct cctctgtata cgaaatattt agaaacgcaa tttgcgcctt tttcactccc
    13621 gcaagggatt ttcaaacagt ggcatacata tgaaaaaaac attactggca gccggtgcgg
    13681 tactggcgct ctcttcgtct tttactgtca acgcagctga aaacgacaaa ccgcagtatc
    13741 tttccgactg gtggcaccag agcgttaacg ttgtcggaag ctatcacacc cgtttcggac
    13801 cgcagatccg caacgatacc taccttgagt acgaagcatt cgctaaaaaa gactggttcg
    13861 acttctatgg ttatgcggat gcgccggtat tcttcggcgg taactccgat gctaaaggta
    13921 tctggaacca cggttctccg ctgtttatgg aaatcgaacc acgtttctcc atcgacaagc
    13981 tgaccaatac tgaccttagc ttcggtccgt tcaaagagtg gtacttcgcg aacaactaca
    14041 tttacgacat gggtcgtaat aaagatggtc gccagagcac ctggtacatg ggtctgggta
    14101 ccgatatcga cactggcctg ccgatgagcc tgtccatgaa cgtctatgcg aaataccagt
    14161 ggcagaacta tggcgcagcg aacgaaaacg agtgggacgg ttaccgtttc aaaattaaat
    14221 actttgtgcc gattaccgat ctgtggggcg gtcagctgag ctacatcggc ttcaccaact
    14281 tcgactgggg ttccgattta ggggatgaca gcggtaacgc aatcaacggt attaagaccc
    14341 gtactaataa ctctatcgct tccagccata ttctggctct gaactacgat cactggcact
    14401 actctgtcgt agctcgttac tggcacgacg gtggtcagtg gaacgacgat gcagaactga
    14461 acttcggcaa cggcaacttc aacgttcgct ctaccggctg gggtggttac ctggtagtag
    14521 gttacaactt ctgattatga aaatgccggg atttattccc ggcatttctg attgttaaca
    14581 acgtgtgctt tgttcatgcc ggatgcggcg taaacgcttt gtccggccca caaaagcacg
    14641 taaattcaat atattgctga aataacgtag gcctgataag cgtagcgcat caggcaattt
    14701 tacgttttaa tcacttcttc ttgttcatca tcgctttcag gtcagcgaat gggttgtact
    14761 tcgcttcacc aacatctttc tgcgcatctt cccctgcgat aacggtcgta ccatactgat
    14821 ccgcttcggt atatttcgaa tgctcatgat cgtggcaata gagacacaac aattcccagt
    14881 tactgccatc ttccgggtta ttggtatggt cgtgatcaat gtggtgaacg gtaagttcac
    14941 gcaggttaga ataaacaaac tcgcgggaac agcgaccgca gacccacgga tagattttta
    15001 atgctttttc gcgatagccg ctttctaacc gtgcatagtt ttttgggatg atagccatga
    15061 aaagttacct ggatgaaatg tagggttacg cgcagtttcc caagatgcgg ggattttcgc
    15121 aatctccata catcaaaaaa aatcccgatc ttctgaccgg gattttcaac atcaacggga
    15181 acttgattaa ggcagaattg acggctgatc cgccccttct ttttccactt tctgctgcaa
    15241 catgtgttcg cgcttcatac ccagtttcag agccaacgca gatgctacgt agatggaaga
    15301 tgcagtgccg atggaaacac cgataagcat ggtcagcgag aagccttcca gtaccggacc
    15361 accgaagaga tacagcatca ggataaccat caaggtagta ccggatgtga tcaaggtacg
    15421 gtgcagcgtc tgggtcaagg acacgttaaa gatttcgtaa ggcgtaccgc gacggatctt
    15481 gcagaagttt tcacgaattc ggtccgatac cacgatactg tcgttaagcg agtaaccgat
    15541 aaccgacatc aacgatgcca caatggtcag gtcaatctcg atatggaata acgacaaaat
    15601 acccagcgta atgataacgt cgtgcgccag cgcaataacc acccctgccg ccagtcgcca
    15661 ctcaaagcgg aaacctacgt acacgaggat agacagcagc gctgccatca acgccatcgc
    15721 accggtttgc gcaaggtctg cccccacgct cggaccgacg aactcaatac gcttcactgc
    15781 tgcattctga ttggtggatt cgttaatcac cttcagaact tggctgccca gcacctgacc
    15841 gccggtttcg ccttcagcag gcggcatacg gaccatgatg tcgtggctgc taccaaagtt
    15901 ttgcagcatt ggctcttcaa aaccggcttt ttgcaatgca tcacgcatta cgtcaatttc
    15961 agccggtttt tcgagcgtaa tttcgataac cgtaccaccg gtgaaatcca gcccccagtt
    16021 aaagccgcgc acgcccataa taacgatagc agcgattaac agcagaccag agatgccgaa
    16081 agcccagtag tcccagcgca taaagtcata gactttacgg ccgtggttta gttgttcaac
    16141 agtatattcc tgtgccacat cgcactcctc agattgacag cttcttgacg cgcttgccgc
    16201 catatagcag gtttacgatg gcacgcgtac cgacaatcgc ggtaaacatc gacgtcgcca
    16261 caccgatacc ggtagtaatc gcgaaccctt taattgcccc ggtacccact gcgtacagga
    16321 tgatgacttt aatcagcgtg gtgatgttcg catcgaagat agaactgaat gcgccacgat
    16381 aaccttcatc aattgcctgt tgaacagtac gtccgttgct caactcttct ttaatacgtt
    16441 cgttgatcag tacgttcgca tcgaccgcta ccgcaagggt taagacgata cccgcgatac
    16501 ctggcatact cagtgttgcg cctggcaaca gcgacataat accgacgatc aagatcaagt
    16561 tggcaatcag agcactggtc gcaatcagac caaacttctt atagaagatg atcatgaaca
    16621 gaatagaaac cagcagaccg gcaaggcaag cctccagccc ctgctcaatg ttctgcatac
    16681 ccagggtcgg gccaattgta cgttcttcaa caatctgaat cggcgcgatc aacgcaccgg
    16741 cacgcagcag cagtgacagc tgacgggctt cgttcgggtt gttgatgccg gtgatacgga
    16801 agctgttacc cagacgagac tggatgttgg cgatgttaat cacctcttcc tgtttcacca
    16861 gaaccgcacg accatttgca tctttcttac cgctgtcttt gtactccaca aacagggttg
    16921 ccatcggttt gccgatgttg tccttggtga agttagacat aatgttgcca ccagcgctat
    16981 cgagcgagat gttaacctgc ggctggttgt attcgtcctg gctggaagtg gagtcggtga
    17041 tatggtcacc agtcagaatt acgcgtttgt acagcacaac cggctgacct tcgcgggtct
    17101 gtttcacttc agagtcgccc ggtacgcgac cggatgccgc agcggcctga tcaacattgg
    17161 tgtttaccag acggaattcc agcgttgccg tcgcgcccag aatctctttc gcacgcgcag
    17221 tgtcctgaat accaggcagt tcaacaacga tacggtcagc accctgacgc tgaaccaccg
    17281 gctccgccac gccaagttgg tttacacggt tacgcaggat attaatgttc tgctgcaccg
    17341 catattcacg cgcttcactc agacgagcat cgctcattac cgcacgcagc tggttgctgc
    17401 cctggctgct aatcaccagg tccggatggc gcttgctcag atacgcaatg gcttcatcac
    17461 gagctttagc atcgcggaaa gtgatgctca ggccgtagtt gttttcttta cgaacagtgg
    17521 tatacgggat gcccttttcg cgcaggtcac tgcgtaggct atcgatattt tgttcctgga
    17581 gtttgccaag cgcggtgtcc atatccactt ccatcaggaa gtgaacgccg ccacgcaggt
    17641 caaggccgag cttcatcggc tcagcatgaa tagctgccag ccagcgcggc gttgccgggg
    17701 caaggttaag cgccacgacg tatttgtcac ccataacgcc cattaatgct tcacgagcgc
    17761 gcaactgggt gtcagtggag tcgaagcgcg caagaatagc gccctcttcc agtgccacag
    17821 acttagcagt tattttttct tcttgtaacg ttttctggac ctggatcagc gtttgctcac
    17881 tggcggcgac tccgcgcgca ccagtgatct gaacagccgg atcctcacca aacaggttgg
    17941 gaagcgcata cagcagaccg atgacaatca ccacgatcag catgacgtac ttccacaaag
    18001 gataacggtt taacacggca attcccttag ggaaaaattt taattacagc gccttcatgg
    18061 tgcctttcgg caggacggca gctacgaagt cacgtttaat aactacttca gtggtgtcat
    18121 tcagcgcgat agcaatgtag ccgttttccg ctactttggt tacgcgacca accaggccac
    18181 cgttcgtcag aacttcatca cctttggcaa tggagtccat cagctttttg tgttctttgg
    18241 tgcgcttctg ctgtggacgc aggatcatga aatagaaaat cagaccgaat accaccagca
    18301 tcaaaatcaa agacatcggg ctaccttgcg ccggtgcacc cgttgccgct accgcatcag
    18361 aaataaaaaa gctcattaaa tttccctcat tattaatatt aatcaacgtt caaaggtggt
    18421 acttctcgcc cctgacgctg gtaaaaatca gttacgaagc tctctaattt accctcttca
    18481 atagccttgc gtaaacccgc catcaaacgc tggtagtaac gaaggttatg aatggtgttg
    18541 agtcgcgcgc ctaatatttc gttgcaacgg tcgagatgat gcaagtaagc gcgtgaataa
    18601 ttgcgacagg tgtagcaatc acactcagga tcgagtgggc cagtatcgct cttatatttc
    18661 gcattgcgga ttttcaccac gccatcggtc acgaacaaat gaccatttcg ggcgttgcgg
    18721 gttggcatta cgcagtcaaa catatcgata ccgcgacgta cgccttcaac caggtcttcc
    18781 ggtttaccaa cgcccatcag gtaacgcggt ttgtctgccg gaatttgcgg acacacatgc
    18841 tccagaatac ggtgcatatc tgctttcggc tcacccacag ccagaccgcc gacagcgtag
    18901 ccatcaaagc cgatatctac cagaccttta acagatatat cacgtaaatc ttcgtaaata
    18961 ctgccctgaa ttataccaaa taacgcattt ttgttaccaa gactgtcaaa acgctcacgg
    19021 ctacgcttcg cccaacgcag agacatctcc atagagcgtt ttgcgtaatc ccagtcagca
    19081 ggatacggcg tacactcatc aaagatcatg acgatatccg aaccaagatc gtactgaatc
    19141 tccatcgact tttccgggtc gaggaaaatc gggtcgccgt tgatcgggtt acggaagtga
    19201 acgccctgtt cggtgatttt acgaatatca ccaaggctga agacctggaa gccgccggag
    19261 tcggtaagaa tcggtccttt ccactgcata aaatcgtgca gatcgccgtg cagtttcatg
    19321 atttcctggc ccgggcgcag ccacaggtgg aaggtgttgc cgaggataat ttgcgcgcca
    19381 gtggcttcaa cttcttccgg cgtcatccct tttacggtgc cgtaggtgcc aacaggcata
    19441 aaacaaggcg tttccactac gccacgatca aagaccaggc ggccacggcg tgcgcgaccg
    19501 tcggtggtgt ccagttcaaa tttcattttt tctccactac gtcagaaaaa cagtccaacg
    19561 tttaaaccag cgccgcggaa ttactccccg acgcgctcat taattgcctg cggattgtac
    19621 gtgataaaca tcgcatcacc gtaactaaaa aagcgatatt tctcttctac cgccgcttta
    19681 taggcattca tggtgtgttg ataaccggca aaggccgaaa ccagcataat cagcgtcgac
    19741 tctggcaagt ggaagttcgt caccagcgca tcgaccactt tgtactggaa gcccggatag
    19801 ataaagattt gggtatcgtc aaagaacggt tcgataagat cgttttttgc cgcctgtgcc
    19861 gcgctttcca gcgaacgtac tgaagtggtg ccaaccgcaa tcacccggtt accgcgcgct
    19921 ttcgccgcca gtaccgcgtc taccacatcc tgcggtactt cagcgtattc cgagtgcatg
    19981 atgtgatctt caatggtgtc gacgcgcacc ggctggaagg tgcccgcacc aacgtgcaac
    20041 gtcacaaacg ccatctccac gcctttggcg cgtaattttt ccagcaaagg ctcgtcaaaa
    20101 tgcagaccag cggtcggggc tgcaaccgcg cccggttttt cgctataaac ggtttgataa
    20161 agttcgcggt cagcgtcttc gtccggacga tcgatatacg gcggcagcgg catatggccg
    20221 atgctgttga ggatatccag taccgagcgt tcatcattaa attcgacttc aaacagtgcg
    20281 ccgtggcgcg cggtcattgt tgcgttgata ctttcgtcat cgcccagcaa cagttctgcg
    20341 ccaggttttg gcgctttcga ggcgcgaata tgcgcaagaa tgcgtttgtc gtcgagcatc
    20401 cgctcaacca gcacttcaat cttgccgccg ctggctttac gcccaaacag acgcgccggg
    20461 atcacgcggg tattattaaa aaccagaaga tcgccggggt tgagcttatc gagtaaatcg
    20521 gtgaaagtac cgtgcgtcag cgcgcccgtc ggcccgtcca gcgacagtaa acgacagcta
    20581 ctgcgttcag gcatgggata gtgggcaatc agggattcgg gcaattcaaa ggagaaatcg
    20641 gtaacgcgca tgacactgac tcagactaaa ataagagtcg ggtagtctag tgccggggcg
    20701 ctctccctgc aacctttacc cccctttgga tagaatataa tcgatgaatt ttttagctca
    20761 cctgcattta gcccatctcg cggaaagctc gctttctggc aatttactgg ctgatttcgt
    20821 acgcggaaat cctgaagaaa gttttccgcc cgacgtcgtg gctggcattc atatgcatcg
    20881 acgtatcgac gtattgactg acaatctgcc ggaagtccgc gaagcacggg agtggtttcg
    20941 tagtgaaacg cgccgcgttg cgcctattac gctggatgtc atgtgggatc actttctttc
    21001 ccgccactgg tcgcagctgt cgccggactt tccgctacag gaatttgtct gttatgcccg
    21061 cgagcaagtg atgacgattt tgccggactc accgccacgt tttatcaatc tgaacaatta
    21121 cttgtggtca gagcagtggc tggtgcgcta tcgcgatatg gatttcatcc agaacgtgtt
    21181 aaacggcatg gcaagccgcc gcccacgtct ggatgccctg cgtgactcct ggtacgattt
    21241 agacgctcat tatgacgccc tcgaaacccg cttctggcag ttttatccgc ggatgatggc
    21301 gcaggcgtca cacaaggcgt tataaaacgt tagttcatcc ataccgtagc cgaaatggca
    21361 ggcaaagcca gaatcccgtc agtcagttgc ccatgccctt ctttgcattg ccattgcacg
    21421 gcattgagaa acggtgacgc gggtagcacc acttcacagg cctcgccacg gttgattgcc
    21481 accagtacac gttgctgatt cagcacgcgg acaaatacca ctacgttatc ttccgcatac
    21541 agcacctgac agccgccacg acgcagcgcc tgacttttct tacgcagcac aatcattcgc
    21601 cggtacagcg cgaataacgc cgtatcctgc ttttccacct gccaggggaa cggtttacgg
    21661 caaaacggat cgtttttacc atccagtccc acttcatcac cgtaataaat gcacggtaca
    21721 ccaggccagg tgaacagcca gaccaccgcc agcggcaggc gcgcaatatc ccgaccgagc
    21781 agcgttttaa atcgcgcagt atcgtggcta tcgagctgat taaacatacg taattgttgt
    21841 tgatgagaaa gccctgcgcg gtaattatcc atccaggcca tacaggtttg ggcgtcaatt
    21901 tgctgcggat cataagagat gtcggtattg gcaagaaatc cccacaacgg gaatgtgaag
    21961 ccacgatagt tcatggcagc atcttccaca tcggcctgta accattgccg tgcatcgcca
    22021 aaatgttcgc cgacaatata cgcgtccggc tgggtttctt tcgccgcttc ggtgatcccg
    22081 gcaacgtgct gcatattatt gcgcgcccca cccgcctccc ccagcatatg caccacatcc
    22141 agccgccagc cgtccatatt ccacggcgct ttcagccagt ggcggacaat actgtcttcc
    22201 ccgcgataaa tttcattcac cagactttcc gactgataat ccagcttcgg caagctggca
    22261 tagccaagcc agtcgagcgc cgtgccatca tcactaaacg agtaccagtc gcgccagggc
    22321 gattcagagt tgtgacaagc tccccccgtg ccaagattgt gcctgtcaaa ccaggcatgg
    22381 gaatcgccac tgtggttaaa cacgccgtcc agcaccagcc gcattcccag ctgctgcgta
    22441 ttgtgtcgca aacgcagcaa caccccatca ccgccaaact gcggatcgac atggcgataa
    22501 tcctcggtat cgtatttatg tacgctggga gctttaaaca ccggattgag atacagcgct
    22561 gtcacgccaa gctttttcag atacggcagt ttttcgctta tcccgtccag atcgccgcca
    22621 tagaacgttg atccgcccgc ctgcgccgtg accggttcat cccagtcacg caagatgatc
    22681 tcttgtccgg ctgcatgatg ataatagaca tgatcctgtt cagcttcacg aggaagacta
    22741 cgcgcaaaac gatcagggaa gatctgataa aaaatctgat ccgcagccca ttgtgggccg
    22801 atatccggta catcgacggc aaactgctcc agtcgtgccg gcggcattcg gctgaagccc
    22861 tgcggtgtaa accaacgctg gcgatcgtgc cacagcagtt tgaaactgta acgccgccgg
    22921 ggttgtccgc tggagagatc aatcgccgca cgccatgcgg tgacgcctgg ctgcggctga
    22981 ctgcgctgct tatgcattgg tactgacatt tcttcgttat cgtgttctgt acgcagcata
    23041 atgcgctgcg gtgggtcttc gcccgtcagc cacagtgtaa tgagcagttg atctttgctt
    23101 tgtttaacaa atgggggcac cggcaggtgc catgcattta acatcataat tcccctgtga
    23161 tgaaaatctg atcaccttgc catagcctaa tgcaggatca ctcatcatct gaaggatatt
    23221 tgggggagga ggacgggggc ggataacttt cggaatagcc ggatcaggat ttcctcctga
    23281 accggcttac agaacgcaag tattattggt tttcagccag ctgacgatcg tggcggcgtt
    23341 taaacatcca gccaatcaac agcacaacaa tccacgcgaa accgacatac agcgagatac
    23401 gcgtatccgg gtgataacca atcaacccga taataaagag caggaaaatc agcccgccga
    23461 tggtcgttgc taccccaccc ggcactttaa atttcagcgc cttaacttct tctggcggca
    23521 aacggcgacg gaaggcgatt tgcgacagca ggatcataat ccacacccat accgtggcga
    23581 aggttgccag cgaagcgatc accaggaaga cgttttccgg catgatgtag ttcagataca
    23641 ccgcaaacag cagcgcggta gtcatcacca gcaccgtaac ccacggaata ccgcgacgcg
    23701 acgttttgct gaaaattttc ggtgcgctgc cctgctctgc cataccgtgg agcatacggc
    23761 ctacgccaaa tacgtcactg ttaattgccg acagcgaagc ggtcagcaca acaaagttaa
    23821 gaatgctggc ggcaaaggta atgcccatat gctggaacgt cagcacgaac gggctaccgg
    23881 cagtgccaac ctgattccac gggtagatag acataatgac gaacagcgta ccgacgtaga
    23941 acaccagaat acgcatcggc acggagttaa tcgcatgcgg aatcgatttc tcaggatctt
    24001 tcgcttcacc ggcggtaatc ccgataattt cgatcccacc gtaagcaaac atcaccattt
    24061 gcaacgacat taccatgcca agccagccgt tactgaagaa gccgccgttg ctccacaggt
    24121 tatgaatacc ggtcggttgc ccgccgttgc caacccccca gatgatgatg ccgaaaccgg
    24181 cgacaatcat gatgatgatg gtggcgactt taaagaacga gaaccagaat tccagctcac
    24241 cgaagacctt cacgctcatc aggtttacgg cgcaaatgat cagcaccacg ctcagtaccc
    24301 aaatccagtg cggcaccgtc gggaacaaga cacccatata gataccaaaa gcggtcacat
    24361 cggcgatggc gacaataagg atttcaaagc agtaggtcca tccggtaatg taacctgcca
    24421 gcgggccaag gttttcctgc gcataacgcg agaaagagct ggcggccggg ttatgtaccg
    24481 acatttcccc cagcgcacgc ataatgatat acgccgcgat accaccgata atataggcca
    24541 acaacacgct cggaccggcc attttgatgg cgtctgccga accgtaaaac agcccggtgc
    24601 caattgctga acccagtgcc ataaagcgta tgtggcgggt acttagccca cgctttagct
    24661 tattcttact ttccatcaat tcctaaccca tcaacacaat aaaaaaccac ggggcatcgc
    24721 agccccgtgg ttaaaacaaa tgttcagtga tttagtgagc gctggaggtc acctgacgac
    24781 ctgccgcgcg atcccagata atggccagaa ccaccatcac cactgttggc attaaccacg
    24841 ccagaccttg ttcggccagc ggtaaacgct gcgcccagga cggtaagata tcgctgaatg
    24901 cagatgcttt gatcccgtcg agaataccaa aaagcaggct gataaacatc ggcggagcaa
    24961 tcacgcggga cgaattatgc caccatgagc gtgtaaaact taatacaacc agtgcgatac
    25021 acggcggata aatagcggtc agcaccggta cggagatctg gatcagctgg cttaagccga
    25081 ggttagacac caccatcgag aagccgccga ggataaacac cagcgtacga taagagagcg
    25141 gtacgtactg ggcaaagaat tctgcacaag cacaggtcag gccaaccgcc gtgaccagac
    25201 aggcgatgaa gattaacgcc gccagcagga agctaccgcc gccgccaaag gtgtgctgaa
    25261 cgtaagcatg cagaatagca gcgccgtttg cagactgatc gaccagcgac gcgctgtctg
    25321 aacccagaca gaacagcgcc aggtacagca aagtcagacc aacacccgcc atcaggccag
    25381 cccagacggt ataacgggtc agcagacgcg cttcggtaac gccacgagaa cgcgccgcgt
    25441 taacaataac gataccaaac accattgcgc ccagcgtatc catggtcaga tagccattaa
    25501 cgaagccgtt agaaaacgca gcgttttgat aagcctcagt cgccgtgctg atagaacccg
    25561 ccggccagac aatcgcggca acagacagga tgaccagcgc gataattttc agcggcgcaa
    25621 ggaagttgcc cacggtatcc agcagcttgc ccggatagag cgaaaccaga ataacgatag
    25681 cgaaatagac caggctgtag ataaacagcg gcagcgcgga atcacccgtc agcggcgcaa
    25741 tccccacttc aaaggaaacg gtagctgtac gcggcgtagc gaaaagcggc cccaccgcca
    25801 ggtaacaaac cgttgccagc agtacgccag cgactttacc gattggcgtg ctgaggctgt
    25861 caacaccgcc gccaactttt gccagcgcca ctaccgttaa taccggcagg ccaacggcag
    25921 taatgaggaa gccgaatgcc gcagtccaga cgtgttcgcc tgcctgtaag ccgaccattg
    25981 gagggaaaat aatgttacct gcgccgacga acaacgcaaa tgtcataaag cccagagcga
    26041 tgatatcgcg cgatcttaat tgatgggtca taaatcatac tgcctgtgga tgtggtgttg
    26101 taacgttaaa tttttgtgcc aatccctggg gatgatacag cggtatattt aaacaatttt
    26161 agcgggaaat actcccaacc aggcgcggcg aagaatagtt tttcgattta ccgcgttaat
    26221 gcagtcagta tgacagcacc tgaagcgcaa tttaaacgct tataacgttt taaagcaagg
    26281 agggatctaa aaaccagagg tatatagcga taagaaatca ccaaccagta taaaacacta
    26341 tttccgtgaa aaagacatgg ctaatcatgc gaacaaaaaa tcaccaaccg ttgtaaaatc
    26401 taacgcccgt attttgactg caaagaaaat aagccagcat cggctggctt atggcaataa
    26461 aagatgacaa aggcggatta atcgctgttt ttggcaatta aacgttccgg gataacaaaa
    26521 ctgaaacgtg ttccttttcc tactgtactc tcaatattca ggcgactttc gtgatgattc
    26581 accgcatgtt tcacgatcgc taaccctaat ccgctaccgc cggtttgccg ggaacgcgct
    26641 ttatcaacgc gataaaaacg ctcggtcagg cgcggaatat gctccggtgc aatgcccggt
    26701 ccgttatctt caacgctaaa ttcggcaccg tgcggcactc gctgccagcg tacggtgata
    26761 tgcgtgcctt ccggcgtatg attcacggcg ttatagacca ggttcgaaat cgcactgcgt
    26821 agctgatctt cgttgccaga caccttgagg ccgttatcta tctcaaaggt aaatgtctgt
    26881 tttttctgac tcagagtctg agcctcgcgc tcaacaacgc gcagcatcat cggcacatca
    26941 accttttcat tgagcaattg cgtcggcgcg gcttcaattt tcgacagcgt tagcaactgc
    27001 ttcaccagcc cttccatccg ctgggtctgc tcgcgcatgg tgtgcaacgc tttttcgcgt
    27061 accgcgcctt ccagcggctg ctcatccatc atctccaggt aaccctgtaa cacggtcaat
    27121 ggcgtacgta actcatggct cacgttggca aaaaagttac gccgcgcccc ttccagttga
    27181 tgcatttgcg tgacatcacg cgccaccatc agcaactgtt tgtgggtata aggcatgacg
    27241 cgaatttcca gatgccgccc ggtgttgagc accagattga gcgggcgaga aaaatcacgc
    27301 gttttcagat attgcgtaaa ctccgggtaa cgcagtaggt taaggatgtt ctgcccgtta
    27361 tcttccggcc agcgcaaacc aagaatttgt tgcgccagac cgttacacca gaaaataccg
    27421 ccctcttccg tggtcagcac caccgcgtcg ggcagcgact ccgcgccgct acgaaagcgt
    27481 ttaatcagat tgcccagttc acggcggcgt tttttatttc gcagctgcat ctggtgtaag
    27541 ccgtatagta gcggttccca gctaccacgc cccggtggcg gggtcatact gcgatccacc
    27601 cacagccacc atgaaaggcg caataaattc cagaaatgcc agataagcag tcctgttacc
    27661 gatgccagca aaaaccaggg caggtaacca aaaaatgcac ccaggatgaa agccgggagg
    27721 cagcaaagta gcagctccag caccagcctt ttccacgaca gccgttccag cacgtaagat
    27781 actccagtta agaaatcata agccctgctc tgcgtccgat gagcaaggcg ttaaaagcgg
    27841 gttgaaaaac gatatcctgt accgcgcacg gtctgcacca tgcggtcatg cccgccgggc
    27901 tccagtgctt tacgcaggcg acgaatgtgg acatcgaccg tgcggtcttc cacatacacg
    27961 ttagttcccc agacgtggtt taacagctgc tcgcggctgt acacgcgctc aggatgcgtc
    28021 ataaaaaagt gcagcagttt aaattctgtc ggccccatct ccagcggctc ttcgcccgcc
    28081 atcactcggt gagatgtcgg gtcgagactt aatccctgca tctcaatcac ctcttccacc
    28141 gccattggcg aaatacggcg cattaccgct ttgattcgcg ccaccagctc cttcggcgaa
    28201 aatggcttgg tgatatagtc atccgcgccg gtttcaaggc cgcgcacgcg atcttcttct
    28261 tcccctctgg cggtcaacat caccactgga atatcccggg tcatcgactc gcgcttgagg
    28321 tgtttgatga actggatacc ggagccgcca ggtaacatcc agtcaaggag aattaaatcc
    28381 ggccagggtt cattcagttg attcacagca ctgtcataat cttccgcttc gaccggctga
    28441 aagccatttt gttcgagcac gaagcagacc atttcgcgaa ttggagcttc atcttctacg
    28501 accagaatac gtctcgccat gatttgccct gttgtaataa ataggttgcg atcattaatg
    28561 cgacgtcatt atgcgtcaga tttatgacag atttatgaaa agctcgtcgc acatatcttc
    28621 aggttattga tttccgtggc gcagaaaaaa gcaaatggca catctgtttg ggtataatcg
    28681 cgaccatgct ttttcgctag ggaaccgtta tgcgcatcct tcacacctca gactggcatc
    28741 tcggccagaa cttctacagt aaaagccgcg aagctgaaca tcaggctttt cttgactggc
    28801 tgctggagac agcacaaacc catcaggtgg atgcgattat tgttgctggt gatgttttcg
    28861 ataccggctc gccgcccagt tacgcccgca cgttatacaa ccgttttgtt gtcaatttac
    28921 agcaaactgg ctgtcatctg gtggtactgg caggaaacca tgactcggtc gccacgctga
    28981 atgaatcgcg cgatatcatg gcgttcctca atacaaccgt ggtcgccagc gccggacatg
    29041 cgccgcaaat cttgcctcgt cgcgacggga cgccaggcgc agtgctgtgc cccattccgt
    29101 ttttacgccc gcgtgacatt atttccagcc aggcggggct taacggtatt gaaaaacagc
    29161 agcatttact ggcagcgatt accgattatt accaacaaca ctatgccgat gcctgcaaac
    29221 tgcgcggcga tcagcctctg cccatcatcg ccacgggaca tttaacgacc gtgggtgcca
    29281 gtaaaagtga cgccgtgcgt gacatttata ttggcacgct ggacgcgttt ccggcacaaa
    29341 actttccacc agccgactac atcgcgctcg ggcatattca ccgcgcacag attattggcg
    29401 gcatggaaca tgttcgctat tgcggttccc ccattccact gagttttgat gaatgcggta
    29461 agagtaaata tgtccatctg gtgacatttt caaacggcaa attagagagc gtggaaaacc
    29521 tgaacgtacc ggtaactcaa cccatggcgg tgctgaaagg cgatctggcg tcgattaccg
    29581 cacagctgga acagtggcgc gatgtatcgc aggagccacc tgtctggctg gatatcgaaa
    29641 tcactactga tgagtatctg catgatattc agcgcaaaat ccaggcatta accgaatcat
    29701 tgcctgtcga agtattgctg gtacgtcgga gtcgtgaaca gcgcgagcgt gtgttagcca
    29761 gccaacagcg tgaaaccctc agcgaactca gcgtcgaaga ggtgttcaat cgccgtctgg
    29821 cactggaaga actggatgaa tcgcagcagc aacgtctgca gcatcttttc accacgacgt
    29881 tgcataccct cgccggagaa cacgaagcat gaaaattctc agcctgcgcc tgaaaaacct
    29941 gaactcatta aaaggcgaat ggaagattga tttcacccgc gagccgttcg ccagcaacgg
    30001 gctgtttgcc attaccggcc caaccggtgc ggggaaaacc accctgctgg acgccatttg
    30061 tctggcgctg tatcacgaaa ctccgcgtct ctctaacgtt tcacaatcgc aaaatgatct
    30121 catgacccgc gataccgctg aatgtctggc ggaggtggag tttgaagtga aaggtgaagc
    30181 gtatcgcgcg ttctggagcc agaatcgggc gcgtaaccag ccggacggta atttgcaggt
    30241 gcctcgcgta gagctggcgc gctgcgccga cggcaaaatt ctcgccgaca aagtgaaaga
    30301 taagctggaa ctgacagcga cgttaaccgg gctggattac gggcgcttca cccgttcgat
    30361 gctgctttcg caggggcaat ttgctgcctt cctgaatgcc aaacccaaag aacgcgcgga
    30421 attgctcgag gagttaaccg gcactgaaat ctacgggcaa atctcggcga tggtttttga
    30481 gcagcacaaa tcggcccgca ctgagctgga gaagctgcaa gcgcaggcca gcggcgtcac
    30541 gttgctcacg ccggaacaag tgcaatcgct gacagcgagt ttgcaggtac ttactgacga
    30601 agaaaaacag ttaattaccg cgcagcagca agaacaacaa tcgctaaact ggttaacgcg
    30661 tcaggacgaa ttgcagcaag aagccagccg ccgtcagcag gccttgcaac aggcgttagc
    30721 cgaagaagaa aaagcgcaac ctcaactggc ggcgcttagt ctggcacaac cggcacgaaa
    30781 tcttcgtcca cactgggaac gcatcgcaga acacagcgcg gcgctggcgc atattcgcca
    30841 gcagattgaa gaagtaaata ctcgcttaca gagcacaatg gcgcttcgcg cgagcattcg
    30901 ccaccacgcg gcgaagcagt cagcagaatt acagcagcag caacaaagcc tgaatacctg
    30961 gttacaggaa cacgaccgct tccgtcagtg gaacaacgaa ctggcgggtt ggcgtgcgca
    31021 gttctcccaa caaaccagcg atcgcgagca tctgcggcaa tggcagcaac agttaaccca
    31081 tgctgagcaa aaacttaatg cgcttgcggc gatcacgttg acgttaaccg ccgatgaagt
    31141 tgctaccgcc ctggcgcaac atgccgagca acgcccactg cgtcagcgcc tggtcgcgct
    31201 gcatgggcag attgttcccc aacaaaaacg tctggcgcag ttaatggtca ctatccagaa
    31261 tgtcactctg gaacagacgc aacgtaatgt cgcacttaac gaaatgcgcc agcgttataa
    31321 agaaaagacg cagcaacttg ccgatgtgaa aaccatttgc gagcaggaag cgcgcatcaa
    31381 aacgctggaa gcccagcgtg cacagttaca ggcgggtcag ccttgtccac tttgtggttc
    31441 caccagccac ccggcggtcg aggcgtatca ggcgctggag cctggtgtta atcagtctcg
    31501 attactggcg ctggaaaacg aagttaaaaa gctcggcgaa gaaggtgcgg cgctgcgcgg
    31561 gcaactggat gcattaacga agcagcttca gcgcgatgaa aacgaagcgc aaagcctccg
    31621 acaagatgag caagcactta ctcaacaatg gcaagccgtc acggccagcc tcaatatcac
    31681 cttgcagcca caggacgata ttcaaccgtg gctggatgca caagatgagc acgaacgcca
    31741 gctgcggtta ctcagccaac gacatgaatt acaagggcag attgccgcgc ataatcagca
    31801 aattatccag tatcaacagc aaattgaaca acgtcagcag cagctcttaa cggcattggc
    31861 gggttatgca ctgacattgc cacaggaaga tgaagaagag agctggttgg cgacacgtca
    31921 gcaagaagcg cagagctggc agcaacgcca gaacgaatta accacgctgc aaaaccgtat
    31981 tcagcagctg acgccgattc tggaaacgtt gccgcaaagt gatgatctcc cgcacagcga
    32041 agaaactgtg gcgctggata actggcggca ggttcatgaa caatgtctcg cattacacag
    32101 ccagcagcag acgttacagc aacaggatgt tctggcggcg caaagtctgc aaaaagccca
    32161 agcgcagttt gacaccgcgc tacaggccag cgtctttgac gatcagcagg cgttccttgc
    32221 ggcgctaatg gatgaacaaa cactaacgca gctggaacag ctcaagcaga atctggaaaa
    32281 ccagcgccgt caggcgcaaa cgctggtcac tcagacagca gaaacgctgg cacagcatca
    32341 acaacaccga cctgacgggt tggctctcac tgtgacggtg gagcagattc agcaagagtt
    32401 agcgcaaact caccaaaagt tgcgtgaaaa caccacgagt caaggcgaga ttcgccagca
    32461 gctgaagcag gatgcagata accgtcagca acaacaaacc ttaatgcagc aaattgctca
    32521 aatgacgcag caggttgagg actggggata tctgaattcg ctaataggtt ccaaagaggg
    32581 cgataaattc cgcaagtttg cccaggggct gacgctggat aatttagtcc atctcgctaa
    32641 tcagcaactt acccggctgc acgggcgcta tctgttacag cgcaaagcca gcgaggcgct
    32701 ggaagtcgag gttgttgata cctggcaggc agatgcggta cgcgataccc gtaccctttc
    32761 cggcggcgaa agtttcctcg ttagtctggc gctggcgctg gcgctttcgg atctggtcag
    32821 ccataaaaca cgtattgact cgctgttcct tgatgaaggt tttggcacgc tggatagcga
    32881 aacgctggat accgcccttg atgcgctgga tgccctgaac gccagtggca aaaccatcgg
    32941 tgtgattagc cacgtagaag cgatgaaaga gcgtattccg gtgcagatca aagtgaaaaa
    33001 gatcaacggc ctgggctaca gcaaactgga aagtacgttt gcagtgaaat aactattcag
    33061 caggataatg aatacagagg ggcgaattat ctcttagcct tgctggtcgt tatcctgcaa
    33121 gctatcactt tattggctac ggtgattggt agccgttctg gtggttgtga tggtggtatg
    33181 aaaaaagtca ttttatcttt ggctctgggc acgtttggtt tggtgatggc cgaatttggc
    33241 attatgggcg tgctcacgga gctggcgcat aacgtaggaa tttcgattcc tgctgccggg
    33301 catatgatct cgtattatgc actgggggtg gtggtcggtg cgccaatcat cgcactcttt
    33361 tccagccgct actcactcaa acatatcttg ttgtttctgg tggcgttgtg cgtcattggc
    33421 aacgccatgt tcacgctctc ttcgtcttac ctgatgctcg ccattggtcg gctggtatcc
    33481 ggctttccgc atggcgcatt ttttggcgtc ggagcgatcg tgttatcaaa aattatcaaa
    33541 cccggaaaag tcaccgccgc cgtggcgggg atggtttccg ggatgacagt cgccaatttg
    33601 ctgggcattc cgctgggaac gtatttaagt caggaattta gctggcgtta caccttttta
    33661 ttgatcgctg tttttaatat tgcggtgatg gcatcggtct atttttgggt gccagatatt
    33721 cgcgacgagg cgaaaggaaa tctgcgcgaa caatttcact ttttgcgcag cccggccccg
    33781 tggttaattt tcgccgccac gatgtttggc aacgcaggtg tgtttgcctg gttctgctac
    33841 gtaaagccat acatgatgtt tatttccggt ttttcggaaa cggcgatgac ctttattatg
    33901 atgttagt
//
LOCUS       DBKEKI010000032        33578 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.032, whole genome shotgun sequence.
ACCESSION   DBKEKI010000032 DBKEKI010000000
VERSION     DBKEKI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.032"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..613
                     /locus_tag="ACUQPX_001502"
     CDS             <1..613
                     /locus_tag="ACUQPX_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9056253.1"
                     /translation="MDIHSYHCEGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQ
                     ELTEVAELITSPEAIKNNLAVAFLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHAS
                     LQQRWSVADMAATIPCSEAWLRRLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGE
                     VADTLNFFDSFHFSKAFKHKFGYAPSAVLKNTDQHPTDASPHN"
     gene            complement(610..1424)
                     /locus_tag="ACUQPX_001503"
                     /pseudo
     CDS             complement(610..1424)
                     /locus_tag="ACUQPX_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1424..3040)
                     /locus_tag="ACUQPX_001504"
     CDS             complement(1424..3040)
                     /locus_tag="ACUQPX_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHV9056254.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3335..4009
                     /locus_tag="ACUQPX_001505"
     CDS             3335..4009
                     /locus_tag="ACUQPX_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHV9056255.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(4048..5709)
                     /locus_tag="ACUQPX_001506"
     CDS             complement(4048..5709)
                     /locus_tag="ACUQPX_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HHV9056256.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(5905..6333)
                     /gene="ibpB"
                     /locus_tag="ACUQPX_001507"
     CDS             complement(5905..6333)
                     /gene="ibpB"
                     /locus_tag="ACUQPX_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HHV9056257.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6445..6858)
                     /gene="ibpA"
                     /locus_tag="ACUQPX_001508"
     CDS             complement(6445..6858)
                     /gene="ibpA"
                     /locus_tag="ACUQPX_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HHV9056258.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(6852..6924)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7163..7495
                     /locus_tag="ACUQPX_001509"
     CDS             7163..7495
                     /locus_tag="ACUQPX_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HHV9056259.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7497..8711)
                     /locus_tag="ACUQPX_001510"
     CDS             complement(7497..8711)
                     /locus_tag="ACUQPX_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HHV9056260.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            8812..9912
                     /gene="cbrA"
                     /locus_tag="ACUQPX_001511"
     CDS             8812..9912
                     /gene="cbrA"
                     /locus_tag="ACUQPX_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HHV9056261.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FNLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(9873..11210)
                     /locus_tag="ACUQPX_001512"
     CDS             complement(9873..11210)
                     /locus_tag="ACUQPX_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9056262.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11285..12433)
                     /gene="dgoD"
                     /locus_tag="ACUQPX_001513"
     CDS             complement(11285..12433)
                     /gene="dgoD"
                     /locus_tag="ACUQPX_001513"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HHV9056263.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRARFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPFGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(12430..13047)
                     /gene="dgoA"
                     /locus_tag="ACUQPX_001514"
     CDS             complement(12430..13047)
                     /gene="dgoA"
                     /locus_tag="ACUQPX_001514"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HHV9056264.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13031..13909)
                     /gene="dgoK"
                     /locus_tag="ACUQPX_001515"
     CDS             complement(13031..13909)
                     /gene="dgoK"
                     /locus_tag="ACUQPX_001515"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HHV9056265.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(13906..14595)
                     /gene="dgoR"
                     /locus_tag="ACUQPX_001516"
     CDS             complement(13906..14595)
                     /gene="dgoR"
                     /locus_tag="ACUQPX_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HHV9056266.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14873..15529
                     /gene="yidX"
                     /locus_tag="ACUQPX_001517"
     CDS             14873..15529
                     /gene="yidX"
                     /locus_tag="ACUQPX_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HHV9056267.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15575..16387)
                     /gene="yidA"
                     /locus_tag="ACUQPX_001518"
     CDS             complement(15575..16387)
                     /gene="yidA"
                     /locus_tag="ACUQPX_001518"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HHV9056268.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16501..16899)
                     /locus_tag="ACUQPX_001519"
     CDS             complement(16501..16899)
                     /locus_tag="ACUQPX_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HHV9056269.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(17129..19543)
                     /gene="gyrB"
                     /locus_tag="ACUQPX_001520"
     CDS             complement(17129..19543)
                     /gene="gyrB"
                     /locus_tag="ACUQPX_001520"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HHV9056270.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19572..20645)
                     /gene="recF"
                     /locus_tag="ACUQPX_001521"
     CDS             complement(19572..20645)
                     /gene="recF"
                     /locus_tag="ACUQPX_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HHV9056271.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPEFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASCLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20645..21745)
                     /gene="dnaN"
                     /locus_tag="ACUQPX_001522"
     CDS             complement(20645..21745)
                     /gene="dnaN"
                     /locus_tag="ACUQPX_001522"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HHV9056272.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21750..23153)
                     /gene="dnaA"
                     /locus_tag="ACUQPX_001523"
     CDS             complement(21750..23153)
                     /gene="dnaA"
                     /locus_tag="ACUQPX_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HHV9056273.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSSWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23450..23530
                     /locus_tag="ACUQPX_001524"
                     /pseudo
     CDS             23450..23530
                     /locus_tag="ACUQPX_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23760..23900
                     /gene="rpmH"
                     /locus_tag="ACUQPX_001525"
     CDS             23760..23900
                     /gene="rpmH"
                     /locus_tag="ACUQPX_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HHV9056274.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            23917..24276
                     /gene="rnpA"
                     /locus_tag="ACUQPX_001526"
     CDS             23917..24276
                     /gene="rnpA"
                     /locus_tag="ACUQPX_001526"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HHV9056275.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24240..24497
                     /gene="yidD"
                     /locus_tag="ACUQPX_001527"
     CDS             24240..24497
                     /gene="yidD"
                     /locus_tag="ACUQPX_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HHV9056276.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24500..26146
                     /gene="yidC"
                     /locus_tag="ACUQPX_001528"
     CDS             24500..26146
                     /gene="yidC"
                     /locus_tag="ACUQPX_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HHV9056277.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26252..27616
                     /gene="mnmE"
                     /locus_tag="ACUQPX_001529"
     CDS             26252..27616
                     /gene="mnmE"
                     /locus_tag="ACUQPX_001529"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HHV9056278.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLTQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEACQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADTTGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            27858..27932
                     /gene="tnaC"
                     /locus_tag="ACUQPX_001530"
     CDS             27858..27932
                     /gene="tnaC"
                     /locus_tag="ACUQPX_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HHV9056279.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            28153..29568
                     /gene="tnaA"
                     /locus_tag="ACUQPX_001531"
     CDS             28153..29568
                     /gene="tnaA"
                     /locus_tag="ACUQPX_001531"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HHV9056280.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQGMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            29659..30906
                     /gene="tnaB"
                     /locus_tag="ACUQPX_001532"
     CDS             29659..30906
                     /gene="tnaB"
                     /locus_tag="ACUQPX_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HHV9056281.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            31038..32213
                     /gene="mdtL"
                     /locus_tag="ACUQPX_001533"
     CDS             31038..32213
                     /gene="mdtL"
                     /locus_tag="ACUQPX_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HHV9056282.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALEIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            32188..33147
                     /gene="yidZ"
                     /locus_tag="ACUQPX_001534"
     CDS             32188..33147
                     /gene="yidZ"
                     /locus_tag="ACUQPX_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HHV9056283.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            33304..33549
                     /locus_tag="ACUQPX_001535"
                     /pseudo
     CDS             33304..33549
                     /locus_tag="ACUQPX_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
BASE COUNT         8095 a         8881 c         8475 g         8127 t
ORIGIN      
        1 aatggatatt cactcttatc actgtgaagg tttagtctgg gaacagtact ggatggaatt
       61 tacccccacc agtatgatgg atattcccgt tggtcagcaa agcgttattt ataatggcga
      121 aatttataat caggaactca ccgaagttgc tgagttaata acttcaccag aagcaataaa
      181 aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt gtcttatgta
      241 cgcagatggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg ccactttaca
      301 tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc cctgtagcga
      361 agcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag aatattacct
      421 cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caaggaaact cggttggcga
      481 agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct ttaaacataa
      541 atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa cggatgccag
      601 tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca actcatccag
      661 aatagctttg gcgaccgagg cgctcggtac cgttttcgac aacgtaatcg cctgccacag
      721 gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg ccacttgctg
      781 actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt caggccgtag
      841 tagcgcacgc gcatctgttt gcgatcttgc agcccaacaa tttgcgccat ccggctttca
      901 ataccgattg gcatgtcaca gatgttgagg attttcgcat tcgggcgcag acgacgcgag
      961 gcttctgcga caattgctgc cggattggag tagttgagca tccaggcgtt tggtgaatat
     1021 ttttccatat aatccaccag ttccaggacg ccgccaatgg aacgcatgcc gtacgctatt
     1081 ccgccgggtc cgcaagtttc ctgaccaaca acaccgtggc gcagtgggat tttttcatcc
     1141 agttcgcgca tcgggtactt gccgacgcgg atgtgcgcca tgacaaaatc aacgtcgctg
     1201 aacgccactt caggatcggt ggtgtaacta aacgcaatgt ccggcgcttt ttctttaagg
     1261 atgactttac aggcttcggc aatcacttcc tgccgcgcgc catcgttatc ataaaatttc
     1321 agtgcacgaa gcgggaaacg gtcctgattc gccaggagca tcaacacgat gcctggcgta
     1381 aaggtgcttc caccgcctgc gacaaccact gagaatttgg tcattatact gcctccgtaa
     1441 tggcaacatt ttctgctgat tgatgagaat taattaagct atcgagctgt tcacgcaatt
     1501 gggatacatg cagaccaatg atcacctgaa tggcatcgcc actacggaag acgccgtgcg
     1561 ctcccagctt tttaaagact tcgtcatcca gcgtttgtga catgtcatgc agtgcaatac
     1621 gtagacgcgt cgcgcaattg ttaatgctgg agatattgcc gaccccgccc agggcttgca
     1681 ggataccggc agcctgatcc agctcttttt ttggctctgc agcggtggtt tggcctcgcg
     1741 aggctttgta ttcggctttt gagtagagtt tcacttccgc atcttcacgt cccggcgtgc
     1801 acatgttgaa ctgcagaatc agggtgcgga aaaccacgaa gtacagcagg gtaaagcaca
     1861 acccaatggc gatttgggtc agcatcatat ccgcgtggtt gctgaacatc gggatccagt
     1921 tttgcggtaa aacctggtca atcagacctc cgcccatgtt gcccaccaca ccaaagagat
     1981 acattacggt cgacattgag gccgccagca cggcgtgtac cgcaaacagc aacggtgaaa
     2041 tgaacaggaa ggtaaattcc agcggttcgg taattcccac cagcatggcg gttaaggttg
     2101 cgggaatcag caagcccgcc acttttaccc gattttccgg tgcggcagtg aagtacatcg
     2161 ctaaagaaat gcccacggca ccaaagattt ttgagttacc gtgcagggca aaaccgcctt
     2221 ccgggaacaa cgatttcagc ggctcggcgc tcagactgaa ctcttgcaga tgctgcgccc
     2281 agtacatctg aatgccgcct tcaacagctg ccggaccaaa gataaacggt ccgtagatga
     2341 agtggtgtaa accggttggg atcagaatac gttcgaggaa ggtgtaaacc cacaccccaa
     2401 gcgcacccgc cgaacgcagg aacgcttgca gagattcaat ccccatttgt acttttggcc
     2461 agccgagcaa tgtcagccag gcacagggga tcatcaccag gaaggcgata atcaccacat
     2521 aagacgtccc ctggaaaatg ccgagaaaaa ccggcagttt tttatcgaac agacggttat
     2581 gcagcgccgt cacaatgccg gaaatgataa ttgcgccgat aatgctggta tcgagggttt
     2641 taatcccggc catcattgtc agaccgctac ctgccaccgc gtcctgagtg aaatcgacgc
     2701 cgaagtagct tccccaggtc attcccatcg cattgatgaa atagttccag gtcaggaaac
     2761 tcaccatcac cgccagacaa gcacgtccct gcgcttgctt agcaaggcca atgggtaaac
     2821 cgacagcaaa aatcagcggc atattacgga ataccgtcca accgccctct tcaataatgt
     2881 gtacgatttg cgcgaataaa ctgttcggat cggtcagtga ttccccgaca aacatcgggt
     2941 tttgcagcaa gatggcaaga cccaccacaa tcccggcgaa gggaaacagc agcactggcg
     3001 tgaacatcgc gccgccaaag cgttgaattt gactgagcat ttccacatcc tcttttctca
     3061 attctgtaag taggaagcct gattggttat gcggcaggcc tggagcgagg ataggaagat
     3121 gcgatcggca gaacatgacg acccaatggg attcgtgatc gctttcatgc ttttcgttgg
     3181 gcggtagaga tctacgtttt cgcgaaaaag tagacatctg tgagcacagg tacacagcat
     3241 cgaacacatc tttaaaaaaa agattttttt caatcgatta agcagaactt gtgggcgcat
     3301 tacccgtgct tgcaggcaaa aaagagagct agagatgatc tacaaaagca ttgcggagcg
     3361 gttaagaatt cgacttaatt ccgcggattt cacgctaaac agccttcttc ccggtgaaaa
     3421 aaagctggcg gaagagtttg cggtatcgcg gatgactatc cgtaaagcca ttgacctgct
     3481 ggtagcgtgg gggctggtgg tccgccgcca cggcagcggc acttacctgg tgcgcaaaga
     3541 tgtgctgcat caaaccgcca gcctgaccgg actggtggag gtgttaaaac ggcagggaaa
     3601 aacggtcacc agccaggtgc tgatttttga aatcatgcct gcgcctccgg ccattgccag
     3661 ccagttacgg attcaaatca acgagcagat ctacttctcc cgtcgcgttc gttttgtgga
     3721 agggaaaccg ctaatgctgg aagacagcta tatgccggta aaactgttcc gtaacctttc
     3781 gctgcaacat ctggaagggt cgaagtttga atatattgaa caagagtgcg ggattttgat
     3841 tggcggtaat tatgaaagcc tgacgccggt gctcgccgat agactgctgg cgcggcaaat
     3901 gaaggtagcg gaacacacgc cactgctgcg gatcacctcg ttgtcatata gcgagagcgg
     3961 ggagtttttg aattattcag tgatgttcag aaatgccagc gaatactagg tggagtacca
     4021 tttacggcga ctccacccgg aaaagagtta accgatactc cagaagagca ccgccagtaa
     4081 ttggggggtg ataatgcgca ggaacatcac taacggatag acagtggcgt aagagagcgc
     4141 cgccgcacca ctggttggat gaagattatt agcaaacgcc agcgccggag gatcggtcat
     4201 ggaacctgcc agcatcccgc acatggtcag gtaattcatt ttggctaaca tccgcgccag
     4261 aatgccaaca gtaatcagcg gaacggcggt gatcagggca ccataaccaa tccagcttag
     4321 cccttcgcca ttgaccaggg tattcacaaa atccccacca gatttcagac caacgaccga
     4381 gaggaacagc acgatcccca gctcccgcag cgcgaggttg gcacttggcg gcataaacca
     4441 gtacagcttg ccgatactgc cgatacgccc gaggatcaac gccataatca gcggaccgcc
     4501 cgccagcccc agtttcaacg cggccgggaa tcctggcaca aagacgggaa tagaacctaa
     4561 caatacgcca agcccgatgc caataaacac tggcagcatc tgaacctgtt gcagtttttg
     4621 ctgcgcattc cccagcacat tggcaacggc atcaattgcg gacggacgcc ccaccaggtt
     4681 gaggatatcg ccgaactgca ggctgatatc gccactggcg accagttcga ccccggcacg
     4741 gttcaggcgc gagataacaa cgtcatagcg ttctttaaag tgcaggtcgc gaatacgttt
     4801 tccgagcacg ttttcattgg tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt
     4861 agacagcgag gtatcgacct cctgaccaat caccagttgc gcattatgta aatccgctgg
     4921 ctgccccacc agatgcagca aatcgcccag ttggataatg gtatctggcg aaggtacttt
     4981 tagggtttct tctcgtttca gacgcgagca gataattttg tcgccgttga gaatcggtac
     5041 atctttaatg gctaaatcat gcaggttagg gttctcaacg cgaatattga tagtcttgat
     5101 cagcgcgccg ccattggtgc gtgaagactc gtgctgctga gcttctgtct cgacattgac
     5161 gcggaaaata acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc cgaatggata
     5221 cgccatcgcg taactcatcc ccatctgatc gaccatttcc attggtgtac ccaggtcgcg
     5281 caagatctgc tgccctgccc ccagcgctgg cgtattggtt accgcaccgg agaaaatccc
     5341 cagcactacc ggcagtggaa tatcaaacag tttatgcagg atggcggtaa ccagaccacc
     5401 gatgatgacg atcagaacag caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc
     5461 aaagaagccc ggccctacct gaatcccgat ggtataaacg aacaggatca ggccaaattc
     5521 ctgaataaca tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc
     5581 gacgatgatc ccaccaaaca gcacgccgcc aatacctaat cctatgccgc gaaatttgac
     5641 gttgccgata aacaaaccga cgactgccac caaagccaga atactgaccg ttaatgctat
     5701 atcactcatc gtccatttct cttgagataa cattttaatt gctacggatt atggctgaat
     5761 cggtgaagca gatttgcctt atagcgcaca ttatcacggg aaagaacgtg ccgaaatatc
     5821 ttaaacagtc gaaatttaac gcattgttga agaagaaaga caaaaaaagc cccaccagaa
     5881 tggcggggca aagagaatag ctagttagct atttaacgcg ggacgttcgc tgatagcgat
     5941 acgctgcgct gcgatgggtt caggctcatt acgaattaaa tcaatatgca gtaaaccgtt
     6001 tacgaaggtt gcgccagaga cttccatatt ttcagccagc gtaaaactca ggctaaatgg
     6061 ctgattcata agcccttgat gcagccattt tttctcttct tttggctgct ccggcgtgcc
     6121 ttttacgctc aggcgcgtac cttccagttg aatctctaaa tcttcctgac ggaaacctgc
     6181 cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt acggcgggaa
     6241 gctctggctt tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac cgatccattg
     6301 acgcatcagt ggggataaat cgaagttacg catagtcatt tctccttcta agaagcgagt
     6361 aagtacctgc aaatccgaag attcgcatat gctccctgac ggcgagcatg gagatgtcag
     6421 gccgcgccag gcggccttag ggaattagtt gatttcgata cggcgcggtt ttttcgcttc
     6481 cggaatcacg cgttcgagat cgatatacag caaaccattt accaggttag ccccacgaac
     6541 atgaatgttc tcagctaact ggaatttgcg ttcaaagttg cgttcagcga tgccctggta
     6601 cagataggtg cgctcttttt gttcgtcggc gtgagcacct ttcaccacca gcagattatc
     6661 ctgggcggta atttccagtt cgctctcagc aaaaccagcc acagcgatag caatgcggta
     6721 atggttttcg tctaccagtt caacgttata cggagggtag ccgccattac tctggctctg
     6781 gttgttttct aagtggttaa acaaacggtc aaatccaata gcagaacggt aaagcgggga
     6841 taaatcaaag ttacgcataa tcaatagctc ctgaaatcag cgagaatgta agaccttcca
     6901 caatggacag gtcaggtagc cagaacaccc atcaggcgcg tcctcatcga ctacgatgta
     6961 aaatgggtct ggaaatgaac ttttcaagcc ttatcagcgg actttttttt gcagtttatg
     7021 gtctattgca tagactgagg gggcagcacg aaacgttaaa gtgcgatagc cgccacagtg
     7081 cgacgtaatg atgatgttat tttcagcaag ggatcgctat aactcatcag gccaacaccc
     7141 acgatcaaca gatgaattta ttatgataag aaatgtgttg ttagcgttca tgatatgcag
     7201 cggaatgaca ttactcggag gatgctccag cgtgatgtcc cataccgggg ggaaagaagg
     7261 aacgtatccc ggcacccgcg ccagcgccac catgattggc gacgatgaaa ccaactgggg
     7321 cactaagtcg ctggcgattc tcgatatgcc gtttaccgct gtgatggaca cgcttttact
     7381 gccgtgggat gtgttccgca aagacagctc ggtgcgctcg cgcgttgaaa aaagcgaagc
     7441 caacgcgcaa gccaccaatg cggttattcc accggccaga atgcctgata actgatttag
     7501 cgatcagttt cggtgatcca cagctggcca ccttccatcc acgccagcca ttgaccatcc
     7561 ggcgagaaga ccacggcgtc cgcagaaggt ggattggcgt ggttttcggt taaatactca
     7621 acctcgccgc tttgcgcatg ggcacaaaca attcgattat ccagcacaaa gcccaaccat
     7681 tctcctgacg gatgccagtt aaatgcagac tgaatatccg ttttgttatg ggttaacttg
     7741 cgcggttcgc cgccctgtgg cgagataagc cacagttgca caatgccgtt atcatcacgc
     7801 attaaaaacg cgatttgcgt accctgcgga ttacagcgca cccagtggcg cgggacgttg
     7861 actaaccccg gataagcccg atgatgggta aaggttaaac gtcgctgcac gacgccacgc
     7921 ggtggcgcgg gtagggttgt ttccgttcca cttaacggcg catcacctgc cgatttccag
     7981 ctagcttcat cttgcggtaa ctcaacgata aatagctccg gcactttctc gccctttggc
     8041 gaaagtgtgt cgccaataaa cgccagcgcg tgatttccta cccatccttc ttcataagca
     8101 cgattgattt catcactgcc aggctgcggc gtgggcgtgg ttttactcac cagcacacac
     8161 cagtggctac cgctgtattc acgcggatgt tgcttttgta cgttgaccgg gccaaacggt
     8221 gcagcaaccc cgacgtttcg caaatccagc gccggatcga gttgatgcat tacatggtcg
     8281 ttataggtaa agctccccct ttcaccgttc gggctaaaga catgcacatg gctgccaccg
     8341 cgcagcgctc ctggcgtata cggcgcggta atatccattg catcgagatt gctcaccttg
     8401 ccgccttcag caatgactcc gcgtcgatga tggaagtcgt aatgccatgt ttcatcagga
     8461 ttttcagggc cgtgaataaa gacatatttc tctgacttcg gatgaacggt caccacgccg
     8521 acgtgtgcgc cctgtgacgc gcgatagata acctcgacct cgcctgtatg gatattcaca
     8581 cgctcaatgg tctcgccggt aaacgacgcc ccagaaggac gcacgtcaaa taccagccac
     8641 tggctgtcgg gcgtccaggt attggtattg gtgagcaggt gattacgggg agcaaaggtt
     8701 atctgtttca tggcgcggtc ctgatacaaa acggttcccg ccatcatact tcacaaggac
     8761 ttcactttca gacaatagtc ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat
     8821 ttcgacgtgg cgattattgg cctcggcccg gcagggtcgg cgttggcacg aaagttagcc
     8881 ggcaaaatgc aggtgatcgc gctggataaa aagcaccagt gcggtactga aggtttcagc
     8941 aaaccttgcg gcggtctgct ggcaccggac gcgcagcgat cttttattcg cgatggactg
     9001 acgcttcctg tcgatgtgat cgccaatcca cagattttca gcgtcaaaac tgtcgacgtc
     9061 gccgcatcgc tcacgcgtaa ctaccagcga agctatatca atattaatcg ccatgctttc
     9121 aacttgtgga tgaaatcgct gatccccgcc agcgttgagg tttatcacga tagcctgtgc
     9181 cgaaaaatct ggcgtgagga tgataaatgg catgtcattt ttcgtgcaga cggctgggag
     9241 caacatatta ccgcccgcta tctggtcggt gccgatggcg caaactcgat ggtgcggcga
     9301 catctctacc cggatcatca aatccgtaaa tatgtcgcta tccagcagtg gttcgcagag
     9361 aaacatccgg tgccgttcta ctcctgcatc tttgataatg cgataactga ctgttactca
     9421 tggagtatca gcaaagatgg ttattttatc tttggcggtg cctatccaat gaaagacggt
     9481 cagacgcgtt tcacgacgct gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa
     9541 gcggtgaaaa gtgagaaatg cacggtgttg tttccctcac gctggcagga ttttgtctgt
     9601 ggtaaggaca acgcctttct gattggtgaa gcggcgggat ttatcagcgc cagctcgctg
     9661 gaggggatta gctatgcgct ggatagcgca gagattctgc gtgcggtgtt cctgaaacag
     9721 ccggagaaga gcaacgccgc ttactggcgc gccacacgca aactgcgttt aaaactcttc
     9781 ggcaagatag taaaaagccg atgcctgacc gcaccggctt taagaaagtg gattatgcgc
     9841 agtggtgtgg cgcatattcc acagttgaaa gattatccaa cgcgcttcac atcacccacc
     9901 agcaggatgt aagagagcgc gccaatcaac gcgacggcgg agatataaac cagtgcaggc
     9961 gcgaaaccgt aaccctgcgc caggtacccc accaccagcg gaacggtgat gccgcccaga
    10021 ccaccggcga agttaaacac gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa
    10081 gagaccagcg accaggtaat cgaagcaaaa ccgttgccga agaatgccag cgccatcagg
    10141 cacataatca tcatcggatc gttggtgtag ttagcgccca taatgcaggt ggagatcagc
    10201 aagccgcaga taatcggcgt tttacgcgca aagcccagtg aaaagccctt acgtaccagc
    10261 agatccgcga cccagccaga gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg
    10321 gtcataaagc ctgctttcag cgccgtgatt cctttttcct gggttaaata gttcgggaac
    10381 caggttaaga aaaaccacag tgcagaagcc accgcaaatt gcccaagata aacgccgatc
    10441 agtttacgat ggaacaccag tttccagtct ttggctgtta acggctgacg cgcctctttc
    10501 ttcaccggcg catcaccatc caccagaccg ccgccatcac gaatgtaatc cagttcagct
    10561 ttgctgatac ctttggtcag gcgcggcggc tgataaacct taaaccaaat cagcgaccag
    10621 ataatgccga taccaccagt gacaatgaac acccagtgcc agctcaacat ctcctgaatc
    10681 cagatcagca gcggagtcaa aaacgccaga ccgacaaact gaccagacgt ataaaaacca
    10741 acggcggaag cgcgttcatg ttccgggaac cagctggtca ccatccggtt attggtcggg
    10801 aaagcgggcg cttcgaaaat accggttatc gcacgcagac caattaatga cattaatccc
    10861 gtggcaaagc cctggaataa agtcgccact gaccaaccaa atatcgcaat aaaataagtc
    10921 acgcgagaac ctacgcgatc taaaaaccaa ccgccgggga tctggcatag cgtataaagc
    10981 caagcgaagg ccgaaaatac atagcccatt tccgctttcg taatgccgaa ctcttcctga
    11041 atatgggcgg aagccacggc caggttagcg cggtcaacat aacaaatgac caccgtaata
    11101 aagatcatca ccagcgtcag ataacgccga cgccccggct ttgctgcatt aacgggaata
    11161 tccatagcga gctttctcca gattttgggc atagcgaagc cgctcaccat gccctgtaat
    11221 ttacagaggg ttatattttt gtattgctgt tttagtgccc gatgaggggc ttacgtggca
    11281 ggaattacca ctctgctacg ctgttatctt catgacgcca gagcggatta cgccagtccg
    11341 gggcattttt actgaactca ataactttag cttcgtcgat ttccacacct aagcccggtt
    11401 tcgttaacgg tttaaagaag ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt
    11461 cgagtaactc cgcgcctttg ttgtaatgaa tgcccatact ttgttcctga agtaccgcgt
    11521 tataggaaac aaagtcgata tgcaggcaag ccgccagtgc aatcggtccg aacggacagt
    11581 gcggcgcaag ggtcacatca taggcttctg ccattccagc aattttgtag cattcggtaa
    11641 taccgcctgc atgggagaga tccggttgca gaatcgaaat accgcctgcc tccagcacgc
    11701 gtttaaaatc gaaacgtgag aacatgcgct cacccgccgc cagtggaata tgcgtttgtg
    11761 ccgccaattt cgggtagtat tcggcttgtt ccgccagcac cggctcttca ataaacagcg
    11821 ggcgatacgg ctccagttct ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt
    11881 ggaaatcaag accaaactca atctgattgc caaaagcttc acgaatttgt gccacggtgt
    11941 tgactgccgc atctaccgcg cgggagttat caattagccc cagttcttca caaccgttca
    12001 gtttgaaggt atcgaagccg atttcgcgca gggttttaat gccgtcgata acatccgccg
    12061 gacgatcgcc gccgacccaa ctgtaggctt taattttgtc gcgaaccagg ccgcccatca
    12121 gttgccagac tggcgcattc agcactttgc ctttgatatc ccataacgcc tggtcgatcc
    12181 cggcgatggc gctcatcagg attggaccgc cacgataaaa tctggcgcga tacatcactt
    12241 gccataagtc gttgatgcgc gaaggatcct gaccaatcaa atagtcaccc agctcgtgaa
    12301 ctgccgcttc caccgtacgg gcgcggcctt caatcacggg ctcgccccaa ccgaccacgc
    12361 cttcatcggt ttcaattttc aggaacatcc agcggggagg taaacgatac gtggtaattt
    12421 tggtgatttt cattgcactg cctctcgata cgccttaaca aatgctgctg cctgctgcgc
    12481 ggtgcgttct acggactgcc cggcgcgata gagatcgctg cctaagcccg ccccaacaca
    12541 acctgcgtct atccactgcg ccaggttttc tggcgtcacg ccgccaacgg caaagactgc
    12601 gatgtccggt ggtaataccg cttttaacgc tttgatgtat tgcggaccaa aagccgatga
    12661 cggaaatatt ttcagcgcct gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt
    12721 cgcgcagccg gggcaaacgg tcatgccgta gcccaccgcg cggcggatca cttcactatg
    12781 gatattgggc gtaacgatga gctgacagcc catcctggcg agcgcatcga cctgttcagg
    12841 tttcagtacc gtacctgcgc caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg
    12901 aatgctctgc tcccattgtg gggaattcag cgggatttca accgcgtcga acccggcgtc
    12961 aatcaccgcg ccaacatgcg ccagcgcctc gtcgggcgta atgccgcgca aaatggcgat
    13021 cagcgggagt ttagtttgcc actgcatgag cgatgctcct tataccagcc tgaaatgccg
    13081 tgtcgcccgc caccgccgcc acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg
    13141 cggtcagcga tgttccggcg acaagggtga tggcgtgttg atgggtcaca tagtcgcgca
    13201 tactggcaac ctctgcgcca atcaacaaac cagagagaaa ttcgctgacc tgttcgcggg
    13261 gaagtgttcc cagcacatgc gaggcgcgaa cttcaaaaag ctgcggcaat atggcgggcg
    13321 cattaaggcc gcgctcaagg ccagccgcga aggcatcggc agagttttcc tgcggcggca
    13381 aacctgcgcc aatcaatgag tgatttaaca gtaaatgatg taattcaccg gtcatcacgg
    13441 tgcgaaaatc gttgatttgc tggctatcgg cctgcaccca tttgcaatgc gtaccgggca
    13501 taacataaag agaggaagga gccagagctc gcgcgccgat caattgtgtt tcttcgccgc
    13561 gcatcacatt gtggttatcg tcatgagaga cgcataatcc gggaataatc cagatattgt
    13621 cgccaacaga cgttaattgt tcgccaatag acgaaaaacg ggcaggaaca gataaatacg
    13681 gagcaacttt ccagccgaca ttgctgccga ccattcctgc catcaccact ggcgtatttt
    13741 cttcacgcca gtcggtcgtg acttctgcta acaccgcagc cggagatttt ccgttcaggc
    13801 gcgtgacgcc tgcttctgat tgcctgctct ccaggcagtg gtcgccctga taaagccagg
    13861 cgcgcagatt ggtcgatccc cagtcaattg cgatgtagcg agctgtcatg tgatttcctt
    13921 taaccttcgt gtcgagctgg caatcatggt aagcgccgcc tgctctgccg catcgccgtc
    13981 ctgatgccgt atcgcatcga acagcgcctt atgttcctgg agcgtttgcg gcatgttggc
    14041 ctcatcgccc atccaggttc gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct
    14101 aagttgctgt aacaccgggt tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc
    14161 cgcttcgtta aacgcttcgc ggtcctgatt gttggcaatc atctcgttca gcgccgattc
    14221 aatctgcgcc agatcgctgg aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc
    14281 caccagattt cgcacttcgc tcatggcact gataagccgt gggtcgtagt cgttttccag
    14341 cacccattgc agtacgtcag tgtcgaggta attccactgg ttacgcggtg ccacaaacgc
    14401 cccgcgataa cgtttcattt caatcagccg cttcgccatc agcgaacgga acacctcacg
    14461 gatgatgttg cgcgaggttg caaactcttc acagagctcc gcctcagccg gaagtggcga
    14521 gccaggtacg tatttgccgt gaacgatctg tttacccagc gtaatgacaa tgcgatcggt
    14581 tttattgaga gtcatggaga gtccttgtgc tctgtatgtt cttctctact ttaccccgat
    14641 cgatgcataa cgcggcaact ttgtagtacc agcgtgatga cgttcgcgtt tgccgtgtgt
    14701 gtaatgtagt acaaacttat attgttgtac tacaatttag atcaaaaaaa gaacaatgcg
    14761 taaaaaaatg acatgcgtcg ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag
    14821 ggaaagaaga gtatgctttc aacggcttag ctactcgttt aaaggattaa tcatgaagtt
    14881 gaattttaag ggatttttta aggctgccgg tttattccca ctggcgctga tgctttcagg
    14941 ctgtatctcg tatgctctgg tttcccatac cgcaaagggt agttcaggaa agtatcaatc
    15001 gcagtcagac accatcactg ggctatcgca ggcaaaagat agtaatggaa caaaaggcta
    15061 tgtttttgta ggggaatcgc tggattacct tatcactgat ggtgccgatg acatcgttaa
    15121 gatgctcaat gatccagcac ttaaccggca caatattcag gttgccgatg acgcaagatt
    15181 tgttttaaat gcggggaaaa agaaatttac cggcacaata tcgctttact actactggaa
    15241 taacgaagaa gaaaaggcac tggcaacgca ttatggtttt gcctgtggtg ttcaacactg
    15301 taccaggtca ctggaaaacc taaaaggcac aatccatgag aaaaataaaa acatggatta
    15361 ctcaaaggtg atggcgttct accatccatt taaagtgcga ttttatgaat actattcacc
    15421 cagaggcatt ccggatggtg tttccgcagc attactgcca gtgactgtta cgctggacat
    15481 cattactgca ccgctgcaat ttctggttgt atatgcagta aaccaataat cagtaagcgg
    15541 gcaaacgcgt ttatgctgtt tgcccgccca cagattaatt cagcacatac ttctcaatag
    15601 caaacgccac gccatcttca aggttggatt ttgtgacaaa gttcgccact tctttcaccg
    15661 aaggaatagc gttatccatc gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt
    15721 tttcctgatc gccaatcgcc atgatttctt ccggtttaat acctaacacg tcggccagtg
    15781 atttcacacc cgtacctttg ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac
    15841 ttttcagcac ggtatatttc tctttcactt cctgcggaat acgcgcgata gcctggtcga
    15901 ggatggcggg ttcatcaatc atcatcactt tcaggaactg ggtattgggg tccattttct
    15961 ccgcttcgca gaacaccagc ggaattgtgg caacgaagga ttcatgcacc gtgtagtagc
    16021 tgatatcgcg gttagcggtg tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga
    16081 cttcgcgaga gagtttttcc aggaaacgat agtcgtcata gctgagagcg gtttgcgcca
    16141 cggtgctacc atcagcggcc ttctgtacca gcgcgccgtt ataagtaatg cagtagtcgc
    16201 ccggctgttc catatgcagc tctttcaggt agttgtgcac acctgcatac ggacgacccg
    16261 tcgttagcac gacattcacg ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg
    16321 gtgaaatggt gtgatcgggc agcagaaggg tgccatccat atcgatagca atgagtttga
    16381 tagccatgag ttcccaaggt agattagttc ctgacccatg ctaacgcgat tccgctcaaa
    16441 aatcagtaca acacccgagg gaaaagggga tgcaacgcgg gttgcatgct ccccatttgc
    16501 ttagcggaag agtttccctt tcagcagttc catgcctgcg gaaagcagat cgttattggc
    16561 ttgtgctgac acttcacctt gcggtgagag cgcatcaata atcttcggca attgctctgc
    16621 cagtaaactg gaagccgtac tggtatccac gccaagtttt tgcccgagat cggacaccgc
    16681 atttgtgccg agcgccgatt ccagttgctc gccgctaact gattgatttc tctgttgatt
    16741 actcaaccag gttgagagaa tggcccctaa gccgccactt tgcagttttt ccagcagcac
    16801 ctgaatgcca ccctgctcct caacccaact taaaatcgcc tgatatttcc ccgcatcgcc
    16861 tttcagaaag gcaccgacaa cttcatcaaa aagccccatg ataatcacct gtaaagcgtt
    16921 acgtgttgac ccaaaaagta tagatctgcg ggatgataat tgcggattgc agaaataaaa
    16981 agggcggaga tgatctccgc ccttttctta tagcttcttg ccggatgcgg cgtgaacgcc
    17041 ttatccggcc tacaaaatca atatattgca ggagtctcgt aggactaata agcgtagcgc
    17101 atcaggcacg ctcgcatggt taacgccatt aaatatcaat attcgccgct ttcagggcgt
    17161 tctcttcaat aaacgcacga cgcggttcta cggcgtcgcc catcagcgtg gtgaacaact
    17221 ggtcagcggc aatcgcatcc ttaacggtaa cgcgcagcat acgacggctt tccgggtcca
    17281 tggtggtttc ccacagctgt tccgggttca tctcgcccag acctttataa cgctggatgg
    17341 agaggccacg acgggactct ttcaccagcc agtccagcgc ctgctcgaag ctggctaccg
    17401 gctgacgacg ctcgccacgt tcgataaacg catcttcttc cagcaagcca cgcagtttct
    17461 cacccagcgt gcagatacga cgatattcgc caccggtgat aaactcgtga tccagcggat
    17521 agtcagtatc cacaccgtgg gtacgcacgc gaacaatcgg ctcgaacagg ttttgttcgg
    17581 cattggtgtg gacatcaaac ttccactggc tgccgtgctg ttctttgtcg ttcagttcgc
    17641 tgaccagcgc gttcacccag cgggtaacgg tctgctcatc agaaaggtca gcttccgtca
    17701 acgtcggctg atagataagc tctttcagca ttgctttcgg ataacgacgc tccatgcggt
    17761 tgatcatttt ctgcgtcgcg ttgtactcag ataccagttt ctctaacgct tcgccagcca
    17821 atgccggtgc actggcgttg gtgtgcagcg ttgcgccgtc cagcgcgata gagatctggt
    17881 actgatccat cgcttcgtcg tctttaatgt actgttcctg cttgcctttc ttcactttgt
    17941 acagcggcgg ctgagcgatg tagacgtgac cgcgttcaac gatttccggc atctgacgat
    18001 agaagaaggt caacagcagc gtacgaatgt gcgagccgtc gacgtccgca tcggtcatga
    18061 tgatgatgct gtgataacgt agtttgtccg ggttgtactc gtcacgaccg ataccacagc
    18121 caagcgcggt gataagcgtc gccacttcct gagaagagag catcttatcg aagcgcgctt
    18181 tctcgacgtt aaggatttta cccttcagcg gcagaatcgc ctggttcttg cggttacgcc
    18241 cctgcttcgc agagccgccc gcggagtccc cttccaccag gtacagttcg gaaagcgccg
    18301 gatcgcgttc ctggcagtct gccagtttgc ccggcaggcc agctaagtcg agcgcacctt
    18361 tacggcgggt catttcacgc gcgcgacgcg ccgcttcacg ggcacgggca gcatcgataa
    18421 ttttgccaac cacgattttc gcatcagttg ggttttccag caggtattcc gccagcagtt
    18481 cgttcatctg ctgttcaacc gccgatttca cctcagaaga aaccagtttg tctttggtct
    18541 gggaggagaa tttcgggtcc ggcactttta cggaaacgac cgcaatcagg ccttcacgcg
    18601 catcgtcacc ggtggcgctg actttggctt ttttgctgta gccttctttg tccatgtagg
    18661 cattcagggt acgggtcatc gccgcacgga agcctgccag gtgagtaccg ccgtcacgct
    18721 gcggaatgtt gttggtaaag cagtagatgt tttcctggaa gccatcgttc cactgcagcg
    18781 ccacttcaac gccaataccg tctttttcgg tggagaagta gaagatattt gggtggatcg
    18841 gcgttttgtt cttgttcaga tattcaacga acgctttgat gccgccttca tagtggaagt
    18901 ggtcttcttt gccgtcacgc ttgtcgcgca gacgaatgga aacgccggag ttgaggaacg
    18961 acaactcacg cagacgtttc gccagaattt catattcgaa ctcggtcaca ttggtgaagg
    19021 tttcgaggct gggccagaaa cgcaccatgg tgccggtttt ttcagtctcg ccggtaaccg
    19081 ccagcggagc ctgcggtaca ccgtgttcgt agatctgacg gtgaatttta ccctcgcgct
    19141 gaataaccag ctccagtttt tgcgacaggg cgtttactac cgaaacacca acgccgtgca
    19201 gaccgccgga cactttatag gagttatcgt caaatttacc gcctgcgtgc agaacggtca
    19261 tgatcacttc cgccgccgat acgccctctt ccgggtgaat accggtcgga atgccgcgcc
    19321 cgtcatcctg tacagagaca gagttatcgg cgtgaatggt gacgataatt tctttacagt
    19381 gacccgcgag cgcttcgtcg atagcgttat ctaccacctc gaataccatg tggtgcagac
    19441 cggtgccgtc atccgtgtcg ccgatataca tacccgggcg cttacgcacc gcatccagcc
    19501 ctttcaggac tttgatactg gaggagtcat aagaattcga catcaacgtt tctcgctcat
    19561 ttatacttgg gttaatccgt tattttaccc ttttccacgg taaacatctt cgaattttcg
    19621 tccgacatgt ctataacgtg ttcagcactg atcgcgctga caaagacctg tgattgcgtc
    19681 gcttttaagc agctggcaag caacccgcga cgctcatcat caagctcaga ggcaaaatca
    19741 tctatcaggt agagacaccg ccgcccgctt tcacgagtga ggaactctcc ttgcgccaga
    19801 cgtaaggcgc acatcaacag cttaagctgc ccacgcgata aggtatcttc caccggcgca
    19861 ccgtcggcgc gaatgcgtaa gtccgcttta tgcgggccat gcgcggtgta ggttagctgg
    19921 cgatcgcgtt caaaattacg ttccagcacc tcagcgtact ctgtctcttt ctcccagccg
    19981 cgctggaaag agaaagtcag agaaaactca gggagaaatt gcttacaggt atcagccata
    20041 tcggccgcga taccggcgct atactccgcg cgccaggtgc tgatttgctc cgccagcggg
    20101 atcagttctt tatcccacgg gcgtagctgt tcgtaacgag tcacctggcg cagcgccgca
    20161 ttgcgctgct tgagcaatcg cttgagattg ctccaggcgg tgaaaaattc aggttcgttg
    20221 tgaaagcatc cccagtcgag gaatgctctt ctgtatttgg ggccgccgtt gagtaaagta
    20281 aacccttctg gcgttatcaa ctgcatcggc atcaggtgcg ccagttccgc gactttatgc
    20341 ccgtccgtac cgtcaatacg gactttgctg tcgccctgtt tgtctttggt taagccaatc
    20401 gccgtctcgc gctcttcgcc ctgtaatcgc ccgtggagaa caaatgcctc ctgctcatgg
    20461 cgaatgacgc gaccaatctg caaactgcga aacgcccgac catggccgag cgtatagatg
    20521 gcttccagca cgctggtttt gccactgccg ttggcaccta ccagaaagtt aaagccggga
    20581 gataaagcga gatccgcggt ttcaatattg cggaaatcgc ggatcaacaa gcgggtgagg
    20641 gacattacag tctcattggc atgacaacat aggcagccga ctgtgatgcg gcatcttcaa
    20701 tctgcacgct ggaaaccgaa tcggtcagca tcatgcggac gttttcgcat ttcagcgcgt
    20761 tcagaacatc cagcacatag ctgacgttga agccgatttc catctccgca ccgctatagg
    20821 taacgtcgag gatctcttcc gcttcttcct gttccgggtt gttggcagta attttcagct
    20881 ggttttcgct gacatacagg cgcacaccgc ggaatttctc gttagagaga atcgccgcgc
    20941 gagcaaacgc ctgcttgagc agatcgcagc ctgcttccag atgtttgtcc gggttcttcg
    21001 gcagaacgcg acggtaatcc gggaagcgac catccaccag tttcgaggtg aaaataaagt
    21061 cgccaacgtg ggcacgaatg ttattgctgc caatctgcac acgcagcaga ttgtcgccgc
    21121 cgtcgagcat acgcatcagt tcaatcacgc ctttacgcgg tacgatcacc gaatggcttg
    21181 gcaaagattg accaattggc attgaacaga ccgccagacg gtggccgtcg gttgccacgg
    21241 tgcgcagttc ttcaccttcg gtttcaaaca gcataccgtt taagtaatag cgaacatcct
    21301 gatgcgccat cgaaaactgg gttgcttcaa tcagacgctt catcgtcgcc tgcggcaggg
    21361 taaattcgac ttcgctctgc cagtcatcga ggttcgggaa atccgccgct ggcagggtag
    21421 acagcgaaaa acggctacgc ccggagcgca ccagcatccg ttcaccttcc agctgcacgg
    21481 caatttccgc gccttcaggc agaccacggc agatatcaaa gaatttgcgc gccggaacgg
    21541 tcgtcgctcc tggctcgtgt ggctgaacca gcgcaacgcg tgccaccatt tccatctcga
    21601 gatcagtacc ggtcagcgac aacgtaccgt cagcaacctg taacagcaga ttaccgagaa
    21661 tcggtagcgt aggacgacca cctaacggac cgctcacctg ttgtagcggt tttaataaat
    21721 gctcacgttc tacggtaaat ttcataggtt tacgatgaca atgttctgat taaatttgaa
    21781 aaatcttctt tgatatcgtg gctctcttca cgcaactgct cgatcttacg gcaggcatga
    21841 agcaccgtcg tgtggtcacg cccaccaaac gcatcgccaa tctctggcag actgtggtta
    21901 gtcagctctt tcgccagcgc catcgccatc tggcgcggac gcgccaccga gcgggatcga
    21961 cgcttggaaa ggagatccgc gactttgatc ttgtagtact ccgccaccgt cttctgaata
    22021 ttgtcgatgg tgaccagttt ttcctgcaat gccagcaagt cgcgcagcgc ctcacgcacg
    22081 aagtcgatgg tgatcgcccg tccggtaaag ttggcgttag caataacgcg gttcagcgcc
    22141 ccttccagct cacgtacgtt agatcgtaga cgcttggcga taaagaacgc cacttcaccc
    22201 ggcaaacgaa tgtcattttc gtcggccttt ttcatcagga tcgccacacg ggtttccagc
    22261 tctggcggtt cgatcgccac agtcagtccc caaccgaagc gggatttcaa acgatcctca
    22321 acgccgttga tctctttcgg atagcgatcc gaggtgagaa tgatctgttg attaccttcc
    22381 agcagggcgt tgaaggtgtg gaaaaactct tcctgagatc gttctttatt agcaaaaaac
    22441 tgaatatcgt cgatcagcag tgcatctacg gaacggtagt agcgtttaaa ctcttcgatc
    22501 gcgttgtttt gcagggcttt aaccatgtcc tgaacaaagc gctcggagtg catataaacc
    22561 actttggcat tcggcttgcg cgccataatg ccgttaccca ccgcatgcag caggtgagtt
    22621 ttacccagac ccgtgccgcc ataaaggaac aacgggttat aggcaccgcc agggttatcc
    22681 gccacctggc gagccgccgc gcgcgccagt tggttagatt taccttcaac gaagttatca
    22741 aacgtgtgtt tgacgtttac gttagaacga taggtcggtt ctgccggggc cgggacgtta
    22801 tcccaacttg agcgcgtaga aggcgcagca cgttgcggct gcgtttgcgc cacctgtgca
    22861 ggggccgcga cgttgctcgt cactgccgct tgtggcgttt gcgtcaccgg tttggtgccg
    22921 acttcaaaac gcagctgtgg ggcatccgct ccgcagaaac tggttagcag tccattgata
    22981 ttattaaggt acttgtcccg tacccaatcg aggacaaaac ggtttggcgc gtacagggcc
    23041 agcgtgttat cgctcagttc cgcctgcaat gggcgtatcc acatactgaa ttctgtggct
    23101 ggtaactcat cctgcaatcg ggcaagacac tgctgccaaa gcgaaagtga cacggcggac
    23161 tccactcgaa caaaagtcga taatgactaa ggctgaaaca ttcatgattg ttgacgtacg
    23221 tcgaaaagac cctgcttgag ggtgacgcac gaaccgctgt ctgcggttat atgcccgatc
    23281 aagatcctgc aaaacgatcg ggaccgcgga tcatagccta aactgcgcaa gagatcttct
    23341 gtttctcaca gattttctct atttatccac aggactttcc agaactcgct aagtgtaaac
    23401 gatcctgccg cgaggcgggc acgatttacg ccgcatattg gaaaatttaa tgaccataga
    23461 caaaaattgg cttaattgat ctaataaaga tccaggacga tccttgcgct ttacccatca
    23521 gcccgtataa tcctccaccc ggcgcgccat gctggtttcc actggtgtga ggtcgtacat
    23581 tttccctgcg aaaaggtgcg gaaaagcgcg gtaaataagg aaagagaatt gactccggtg
    23641 tgtacaatta ttacaatccg gcctctttaa tcacccatgg cttcggtgtc catcgtttca
    23701 tttttcggcg gatatccaat aaagccattg aatttattca agtttaggta gaaatcgcca
    23761 tgaaacgcac ttttcaaccg tctgtactga agcgcaaccg ttctcacggc ttccgtgctc
    23821 gtatggctac taaaaatggt cgtcaggttc tggcacgtcg tcgtgctaaa ggccgcgctc
    23881 gtctgaccgt ttctaagtaa taaagctaac ccctgagtgg ttaagctcgc atttcccagg
    23941 gagttacgct tgttaactcc cagtcaattc acattcgtct tccagcagcc acaacgggct
    24001 ggcacgccgc aaattaccat tctcggccgc ctgaattcgc tggggcatcc ccgtatcggt
    24061 cttacagtcg ccaagaaaaa cgttcgacgc gcccatgaac gcaatcggat taaacgtctg
    24121 acgcgtgaaa gcttccgtct gcgccaacat gaactcccgg ctatggattt cgtggtggtg
    24181 gcgaaaaaag gggttgccga cctcgataac cgtgctctct cggaagcgtt ggaaaaatta
    24241 tggcgccgcc actgtcgcct ggctcgcggg tcctgatagc cctcattcgg gtctatcaac
    24301 gcctgattag tccgctactc gggccgcatt gtcgtttcac tccaacctgt tcaagctacg
    24361 gaattgaggc attgcgcagg tttggagtga taaaaggcag ttggttgacg gtgaaacgcg
    24421 tattaaaatg ccacccttta caccctggtg gtgacgatcc cgtcccgccc ggaccatttg
    24481 ataccagaga acactaacga tggattcgca acgcaatctt ttagtcatcg ctttgctgtt
    24541 cgtgtctttc atgatctggc aagcctggga gcaggataaa aacccgcaac ctcaggccca
    24601 acagaccacg cagacaacga ccaccgcagc gggtagcgcc gccgaccagg gcgtaccggc
    24661 cagtggccag gggaaactga tctcggttaa gaccgacgtg cttgatctga ccatcaacac
    24721 ccgtggtggt gatgttgagc aagctctgct gcctgcttac ccgaaagagc tgaactctac
    24781 ccagccgttc cagctgttgg aaacttcacc gcagtttatt tatcaggcac agagcggtct
    24841 gaccggtcgt gatggcccgg ataacccggc taacggcccg cgtccgctgt ataacgttga
    24901 aaaagacgct tatgtgctgg ctgaaggtca aaacgaactg caggtgccga tgacgtatac
    24961 cgacgcggca ggcaacacgt ttaccaaaac gtttgtcctg aaacgtggtg attacgctgt
    25021 caacgtcaac tacaacgtgc agaacgctgg cgagaaaccg ctggaaatct cctcgtttgg
    25081 tcagttgaag caatccatca ctctgccacc gcatctcgat accggaagca gcaacttcgc
    25141 actgcacacc ttccgtggcg cggcgtactc cacgcctgac gagaagtatg agaaatacaa
    25201 gttcgatacc attgccgata acgaaaacct gaacatctct tcgaaaggtg gttgggtggc
    25261 gatgctgcaa cagtatttcg cgacggcgtg gatcccgcat aacgacggta ccaacaactt
    25321 ctataccgct aatctgggta acggcatcgc cgctatcggc tataaatctc agccggtact
    25381 ggttcagcct ggtcagactg gcgcgatgaa cagcaccctg tgggttggcc cggaaatcca
    25441 ggacaaaatg gcagctgttg ctccgcacct ggatctgacc gttgattacg gttggttgtg
    25501 gttcatctct cagccgctgt tcaaactgct gaaatggatc catagctttg tgggtaactg
    25561 gggcttctcc attatcatca tcacctttat cgttcgtggc atcatgtacc cgctgaccaa
    25621 agcgcagtac acctccatgg cgaagatgcg tatgctgcag ccgaagattc aggcaatgcg
    25681 tgagcgtctg ggcgatgaca aacagcgtat cagtcaggaa atgatggcgc tgtacaaagc
    25741 tgagaaggtt aacccgctgg gcggctgctt cccgctgctg atccagatgc caatcttcct
    25801 ggcgttgtac tacatgctga tgggttccgt tgaactgcgt caggcaccgt ttgcactgtg
    25861 gatccacgac ctgtcggcac aggacccgta ctacatcctg ccgatcctga tgggcgtaac
    25921 gatgttcttc attcagaaga tgtcgccgac cactgtgacc gacccgatgc agcagaagat
    25981 catgaccttt atgccagtca tcttcaccgt gttcttcctg tggttcccgt caggtctggt
    26041 actgtactat atcgtcagca acctggtaac cattattcag cagcagctga tttaccgtgg
    26101 tctggaaaaa cgtggcctgc atagccgcga gaagaaaaaa tcctgattcg gtgagttttc
    26161 gctaaaataa gggcggtcag ttgaccgcct tttttctttt cgtagggcgg ataagcaccg
    26221 cgcatccgcc acacaaagca acaggaacat catgagcgat aatgacacta tcgtagccca
    26281 ggccacgcct ccgggacgtg gcggcgttgg catcctgcgc atctccggct tcaaagcccg
    26341 tgaagttgcc gaaaccgtgc tgggtaaact gcctaagccg cgctacgccg attatcttcc
    26401 gtttaaagac gccgacggca gcgtgctcga tcaggggatt gcgctatggt tccctggccc
    26461 gaactcgttc accggcgaag atgtgctgga actgcaaggt catggcggtc cggtgatcct
    26521 cgacctgctg ttaaaacgca ttctgaccat tcccggcctg cggattgctc gccctggtga
    26581 gttttccgaa cgcgcgtttc ttaacgataa acttgactta acccaggccg aggcgattgc
    26641 cgatcttatc gacgccagtt cggaacaggc ggcccgttcg gcacttaact cgctgcaagg
    26701 cgcattctcc gcacgggtta atcatctggt agaagccctc acccacttgc gcatttacgt
    26761 cgaagcggca attgatttcc ctgatgaaga gatcgatttc ctctccgacg gcaaaattga
    26821 agcccagctt aatgacgtga tggccgatct cgatgcagtg cgtgctgaag catgtcaggg
    26881 tagtttgttg cgcgaaggga tgaaagtagt gattgccgga cgtcctaatg ccggtaaatc
    26941 gagcctgtta aacgcgctgg cgggccgtga agcggcaatc gtaaccgata tcgccggaac
    27001 cacgcgtgac gtgctgcgtg agcatatcca cattgacgga atgccgctgc atatcatcga
    27061 cactgccggg ctacgtgagg ccagtgacga agtggaacgt attggtatcg agcgcgcgtg
    27121 gcaggaaatt gaacaggccg accgcgtgct gtttatggtc gatggcacca cgacagacgc
    27181 cgttgatccg gcagagatct ggccggaatt tatcgcccgt ctgcccgcga aactgccgat
    27241 caccgtggta cgcaataaag ccgataccac cggcgaaacg ctgggaatga gtgaagtgaa
    27301 cggtcacgcg ttaattcgtc tctcggcaag gactggtgaa ggcgtggacg tgctgcgtaa
    27361 ccatctcaaa cagagcatgg gctttgacac caatatggaa ggcggcttcc tggcgcgccg
    27421 tcgccactta caggcgctgg aacaggcagc ggagcatttg cagcaaggta aagcgcaact
    27481 gttgggagcc tgggcaggtg aactgctggc ggaagagttg cgcctggcac agcagaactt
    27541 aagcgaaatc accggggaat ttacttcaga cgacctgctg gggcggattt tctccagctt
    27601 ctgtattggt aagtaaccgc gcttacgaag ccgtattctg actgtcagat gcggcttcgc
    27661 ttcattgtta gcactcctgt tatgcctcaa ccctttttta aacattaaaa ttcttacgta
    27721 atttataatc tttaaaaaaa gcatttaata ctgctccccg aacgattgtg attcgattca
    27781 catttaaaca atttcagaat agacaaaaat tctgagtgta ataatgtagc ggcgtgtctt
    27841 tcgaggataa gtgcattatg aatatcttac atatatgtgt gacctcaaaa tggttcaata
    27901 tcgacaataa gattgtcgat caccgccctt aatttaccct tctgtagcca tcaccagagc
    27961 caaaccgatt agattcaatg tgatcgatta gtttgctata tcttaatttt gccttttgca
    28021 aaggccatct ctcgtttatt tacttatttt agtaaatgat ggtgcttgta tatatacatg
    28081 gcgaattaat ggatattgca gatgtaatat tcacagggat cactgtaatt aaaataaatg
    28141 aaggattatg taatggaaaa ctttaaacat ctccctgaac cgttccgcat tcgtgttatt
    28201 gagccagtaa aacgtactac tcgcgcttat cgtgaagaag caattattaa atccggtatg
    28261 aacccgttcc tgctggatag cgaagatgtg tttatcgatt tactgaccga cagcggcacc
    28321 ggggcggtaa cccaaggtat gcaggcagcg atgatgcgcg gcgacgaagc ctacagcggc
    28381 agccgcagct actatgcgtt agccgagtca gtgaaaaata tctttggtta tcaatatact
    28441 attccgactc accagggccg tggcgcagag caaatctata ttccggtact gattaaaaaa
    28501 cgcgagcagg aaaaaggcct ggatcgcagc aaaatggtgg cattctctaa ctatttcttt
    28561 gataccacgc agggccatag ccagattaac ggctgtaccg tgcgtaacgt ctatatcaaa
    28621 gaagccttcg atacgggcgt gcgttacgac tttaaaggca actttgacct cgaaggatta
    28681 gaacgcggta ttgaagaagt tggcccgaat aacgtgccgt atatcgttgc aaccatcacc
    28741 agtaactctg caggtggtca gccggtttca ctggcaaact taaaagcgat gtacagcatc
    28801 gcgaagaaat acgatattcc ggtggtaatg gactccgcac gctttgctga aaacgcctat
    28861 ttcatcaagc agcgtgaagc agaatacaaa gactggacca tcgagcagat cacccgcgaa
    28921 acctacaaat atgccgatat gctggcgatg tccgccaaga aagatgcgat ggtgccgatg
    28981 ggcggcttgc tgtgcatgaa agacgacagc ttctttgatg tgtacaccga gtgcagaacc
    29041 ctttgcgtgg tgcaggaagg cttcccgaca tatggcggcc tggaaggcgg cgcgatggag
    29101 cgtctggcgg taggtctgta tgacggcatg aatctcgact ggctggctta tcgtatcgcg
    29161 caggtacagt atctggtcga tggtctggaa gagattggcg ttgtctgcca gcaggcgggc
    29221 ggtcacgcgg cattcgttga tgccggtaaa ctgctgccgc atatcccggc agaccagttc
    29281 ccggcacagg cgctggcgtg cgagctgtat aaagtcgccg gtatccgtgc ggtagaaatt
    29341 ggctctttcc tgttaggccg cgatccgaaa accggtaaac aactgccatg cccggctgaa
    29401 ctgctgcgtt taaccattcc gcgcgcaaca tatactcaaa cacatatgga cttcattatt
    29461 gaagccttta aacatgtaaa agagaacgcg gcgaatatta aaggattaac ctttacctac
    29521 gaaccaaaag tattgcgtca cttcaccgca aaactgaaag aagtttaatt aatactacag
    29581 agtggctata aggatgttag ccactctctt accctacatc ctcaataaca aaaatagcct
    29641 tcctctaaag gtggcatcat gactgatcaa gctgaaaaaa agcactctgc attttggggt
    29701 gttatggtta tagcaggtac agtaattggt ggaggtatgt ttgctttacc tgttgatctt
    29761 gccggtgcct ggtttttctg gggtgccttt atccttatca ttgcctggtt ttcaatgctt
    29821 cattccgggt tattgttatt agaagcaaat ttaaattatc ctgtcggctc cagttttaac
    29881 accatcacca aagatttaat aggtaacacc tggaacatta tcagcggtat taccgttgcc
    29941 ttcgttctct atatcctcac ttatgcctat atctctgcta atggtgcgat cattagtgaa
    30001 acgatatcaa tgaatttggg ctatcacgct aatccacgta ttgtcgggat ctgcacagcc
    30061 attttcgttg ccagcgtatt gtggataagt tcgttagccg ccagtcgcat tacctcattg
    30121 ttcctcgggc tgaagattat ctcctttgtg atcgtgtttg gttctttctt cttccaggtc
    30181 gattactcca tcctgcgcga tgccaccagc accactgcgg gaacgtctta cttcccgtat
    30241 atctttatgg ctttgccggt gtgtctggcg tcatttggtt tccacggcaa tattcccagc
    30301 ctgattattt gctatggcaa acgcaaagat aagttaatca aaagcgtggt atttggttcg
    30361 ctgctggcgc tggtgattta tctcttctgg ctctattgca cgatggggaa tattccgcgc
    30421 gaaagcttta aggcgataat ctcctcaggc ggcaacgttg attcgctggt gaaatcgttc
    30481 ctcggcacca aacagcacgg cattatcgag ttttgcctgc tggtgttctc taacttagct
    30541 gttgccagtt cgttctttgg tgtcacgctg gggttgttcg attatctggc ggacctgttt
    30601 aagattgata actcccacgg cgggcgtttc aaaaccgtgc tgttaacctt cctgccacct
    30661 gcgttgttgt atctgatctt cccgaacggc tttatttacg ggatcggcgg tgccgggctg
    30721 tgcgccacca tctgggcggt cattattccc gcagtgcttg caatcaaagc tcgcaagaag
    30781 tttcccaatc agatgttcac ggtctggggc ggcaatctta ttccggcgat tgtcattctc
    30841 tttggtataa ccgtgatttt gtgctggttc ggcaacgtct ttaacgtgtt acctaaattt
    30901 ggctaaatcc ttcaagaagc cagccattcg ctggcttctt gcctctcagg aaatcactta
    30961 tgtccaaatg gcaactcgcc ttatcctcct tcaccacgta tgctttgcgt caccttacta
    31021 tcaggacgct ttagcccatg tcccgctttt tgatttgtag ttttgccctg gttttacttt
    31081 atcccgccgg gattgatatg tacctcgttg gtttaccgcg catcgccgcc gatctcaatg
    31141 ccagcgaagc gcagttgcat attgcgttct ccgtatatct ggcggggatg gcagctgcga
    31201 tgctatttgc cggtaaagtg gccgatcgtt cagggagaaa gccggtcgcc atacccggcg
    31261 cggcgctatt tattattgcc tcggtgttct gttcactggc tgaaaccagc acgttatttc
    31321 ttgcaggccg atttctacag gggttgggcg caggctgttg ttacgtggtg gcgttcgcca
    31381 ttttgcgcga cacgctggat gatcgacgtc gggctaaagt gctgtcatta ctcaacggta
    31441 ttacctgcat cattccggtg ttagcgccag tgctcggaca tctgattatg cttaaattcc
    31501 cgtggcagag tctgttctgg gcgatggcaa tgatgggcat cgcggtactg atgttgtctt
    31561 tgtttatttt aaaagaaacg cgcccagcgt cccccgctgc ttcggacaaa ccacgagaaa
    31621 atagcgagtc gctgcttaat cgttttttcc tcagccgtgt tgttatcacc accctcagcg
    31681 tttcggtgat cctcactttc gtcaatacat cgccggtatt gctgatggaa atcatggggt
    31741 ttgagcgcgg agaatacgcc accattatgg cgctgaccgc tggcgtcagc atgacctttt
    31801 cattctcgac gccatttgcg ctggaaattt ttaagccacg tacgttgatg atcacctcgc
    31861 aggtgttatt ccttgcggcg gggatcaccc ttgccgtttc accttcccat gcggtttctc
    31921 tgtttggtat cacgctgatt tgcgccggtt tctcggtagg ttttggcgta gcgatgagtc
    31981 aggcgttagg accattttca ttacgcgcgg gcgtagccag ctcgacctta ggtattgcgc
    32041 aggtttgcgg ttcgtcactg tggatttggc tggcagcggt ggttggtatc ggcgcatgga
    32101 atatgctgat cgggattctg attgcctgta gcatagtgag cctgttgctg attatgttcg
    32161 tcgcgcctgg acgccccgtt gccgctcatg aagaaatcca tcaccacgct tgatctcaat
    32221 ctgctgctct gtctgcaact gctgatgcag gagcgcagcg tgaccaaagc ggcgaagcgg
    32281 atgaacgtga ccccttcagc ggtgagtaag tcgctggcaa agttaagagc gtggtttgac
    32341 gacccgctct ttgtgaactc accgctgggg ctgtcgccca caccgctaat ggtcagcatg
    32401 gagcaaaatc tggcggaatg gatgcaaatg agcaatctgc tgctggataa accgcaccac
    32461 cagacaccgc gcggcctgaa gtttgagttg gcggcggaat cgccgctgat gatgatcatg
    32521 cttaatgcgc tgtcgaaacg gatctaccaa cgttacccgc aggcgaccat caaactacgt
    32581 aactgggatt acgattcctt agatgccatt actcgtggtg aagtggatat tggtttttcc
    32641 ggtcgcgaaa gccatcctcg ctcgcgggag ctgttaagct cgctaccgtt agccattgat
    32701 tatgaagtgc tgtttagtga tgtaccctgc gtctggttac gcaaagatca tccggcactg
    32761 catgaaacgt ggaatctgga caccttttta cgttatccgc atatcagcat ttgctgggaa
    32821 cagagcgaca cctgggcgct ggacaatgtg ttacaggagc tggggcgcga acgcacgatt
    32881 gctatgagcc tgccggaatt cgagcagtca ctgtttatgg cagcgcaacc cgacaatctg
    32941 ctactggcga ccgcgccgcg ctactgtcag tactacaatc aactccatca actgccgttg
    33001 gttgctcttc ctctcccgtt tgacgaaagc cagcaaaaaa agctggaagt tccttttacc
    33061 ctgctgtggc ataaacggaa cagccataat ccgaagatcg tctggttacg ggaaaccatt
    33121 aaaaaccttt acgcgtcgat ggcataaccg aatcgtatga aacgcgaccc aatttcacaa
    33181 taaaatgtaa aaaagttgta ataagcttgt ctgaatcgaa cttttagccg ctttagtctg
    33241 tccatcattc cagtaaatga ttactcttgt attcataatg gaccattaag catggagcga
    33301 aaaatggcga ctcactttgc ccgagggatt ttaacggaag gacatctgat ttctgttcgt
    33361 ctcccctccc agtgtcatca agaagcccga aacattcccc ctcatcgtca aagccgtttt
    33421 ctggcgtcca gaggtttact cgcagaactg atgttcatgc tgtatggcat tggcgaattg
    33481 ccggaaatcg tcaccctgcc gaaaggtaaa ccggttttca gtgataaaaa tttgccttcg
    33541 ttttctatgt aagcgcccca tcagcgacgt cttgtgaa
//
LOCUS       DBKEKI010000033        33575 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.033, whole genome shotgun sequence.
ACCESSION   DBKEKI010000033 DBKEKI010000000
VERSION     DBKEKI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.033"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            349..1158
                     /locus_tag="ACUQPX_001536"
                     /pseudo
     CDS             349..1158
                     /locus_tag="ACUQPX_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1355..2134
                     /gene="ipaJ"
                     /locus_tag="ACUQPX_001537"
     CDS             1355..2134
                     /gene="ipaJ"
                     /locus_tag="ACUQPX_001537"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HHV9056284.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3081..4010)
                     /gene="virB"
                     /locus_tag="ACUQPX_001538"
     CDS             complement(3081..4010)
                     /gene="virB"
                     /locus_tag="ACUQPX_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HHV9056285.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4227..4463)
                     /locus_tag="ACUQPX_001539"
     CDS             complement(4227..4463)
                     /locus_tag="ACUQPX_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HHV9056286.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4467..6368)
                     /gene="ipaA"
                     /locus_tag="ACUQPX_001540"
     CDS             complement(4467..6368)
                     /gene="ipaA"
                     /locus_tag="ACUQPX_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HHV9056287.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSLGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQPDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6377..7375)
                     /gene="ipaD"
                     /locus_tag="ACUQPX_001541"
     CDS             complement(6377..7375)
                     /gene="ipaD"
                     /locus_tag="ACUQPX_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HHV9056288.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSFHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDTLSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKNALEELKKKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7426..8574)
                     /gene="ipaC"
                     /locus_tag="ACUQPX_001542"
     CDS             complement(7426..8574)
                     /gene="ipaC"
                     /locus_tag="ACUQPX_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HHV9056289.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKSTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIAHLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMDTSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSITQSKNSTASQIAGNIRA"
     gene            complement(8537..10279)
                     /gene="ipaB"
                     /locus_tag="ACUQPX_001543"
     CDS             complement(8537..10279)
                     /gene="ipaB"
                     /locus_tag="ACUQPX_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HHV9056290.1"
                     /translation="MHNVSTTTTGLSLDKILTSTELGDNTIQAANDAANKLFSLTIAD
                     LTANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPDSPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KSFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10285..10752)
                     /gene="ipgC"
                     /locus_tag="ACUQPX_001544"
     CDS             complement(10285..10752)
                     /gene="ipgC"
                     /locus_tag="ACUQPX_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HHV9056291.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10810..11436)
                     /gene="ipgB1"
                     /locus_tag="ACUQPX_001545"
     CDS             complement(10810..11436)
                     /gene="ipgB1"
                     /locus_tag="ACUQPX_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HHV9056292.1"
                     /translation="MQILNKILPQVEFAIPRPSFNSLSYNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDNVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11452..11841)
                     /gene="ipgA"
                     /locus_tag="ACUQPX_001546"
     CDS             complement(11452..11841)
                     /gene="ipgA"
                     /locus_tag="ACUQPX_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HHV9056293.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11854..13338)
                     /gene="icsB"
                     /locus_tag="ACUQPX_001547"
     CDS             complement(11854..13338)
                     /gene="icsB"
                     /locus_tag="ACUQPX_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HHV9056294.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPMLVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEE
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13652..15268
                     /locus_tag="ACUQPX_001548"
     CDS             13652..15268
                     /locus_tag="ACUQPX_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HHV9056295.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNPKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTKYLNNKNWEPINKNINY
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESQHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRRELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKGLNSKRGEPTKLLIRNSDGLLQEVNVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKDIVNKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHDTGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            15278..15640
                     /gene="ipgE"
                     /locus_tag="ACUQPX_001549"
     CDS             15278..15640
                     /gene="ipgE"
                     /locus_tag="ACUQPX_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HHV9056296.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15640..16098
                     /gene="ipgF"
                     /locus_tag="ACUQPX_001550"
     CDS             15640..16098
                     /gene="ipgF"
                     /locus_tag="ACUQPX_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HHV9056297.1"
                     /translation="MSRFVFILLFFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESR
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            16107..17222
                     /gene="mxiG"
                     /locus_tag="ACUQPX_001551"
     CDS             16107..17222
                     /gene="mxiG"
                     /locus_tag="ACUQPX_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HHV9056298.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICQFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICEANKVTYSVREELTDEKLELINRLISDHKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17230..17481
                     /locus_tag="ACUQPX_001552"
     CDS             17230..17481
                     /locus_tag="ACUQPX_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HHV9056299.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGELTLALDALARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNYR"
     gene            17494..17787
                     /gene="mxiI"
                     /locus_tag="ACUQPX_001553"
     CDS             17494..17787
                     /gene="mxiI"
                     /locus_tag="ACUQPX_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HHV9056300.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVGNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17793..18518
                     /gene="mxiJ"
                     /locus_tag="ACUQPX_001554"
     CDS             17793..18518
                     /gene="mxiJ"
                     /locus_tag="ACUQPX_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HHV9056301.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSEVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYVFLG
                     MAVLVVTLLVWAFKTGWFKRNKI"
     gene            18515..19042
                     /locus_tag="ACUQPX_001555"
     CDS             18515..19042
                     /locus_tag="ACUQPX_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HHV9056302.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18984..19679
                     /gene="mxiN"
                     /locus_tag="ACUQPX_001556"
     CDS             18984..19679
                     /gene="mxiN"
                     /locus_tag="ACUQPX_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HHV9056303.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIINTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19672..20079
                     /gene="mxiM"
                     /locus_tag="ACUQPX_001557"
     CDS             19672..20079
                     /gene="mxiM"
                     /locus_tag="ACUQPX_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HHV9056304.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFDAGREADDSTW"
     gene            20063..20491
                     /gene="mxiM"
                     /locus_tag="ACUQPX_001558"
     CDS             20063..20491
                     /gene="mxiM"
                     /locus_tag="ACUQPX_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HHV9056305.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20621..21253
                     /gene="mxiE"
                     /locus_tag="ACUQPX_001559"
     CDS             20621..21253
                     /gene="mxiE"
                     /locus_tag="ACUQPX_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HHV9056306.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21240..22940
                     /gene="mxiD"
                     /locus_tag="ACUQPX_001560"
     CDS             21240..22940
                     /gene="mxiD"
                     /locus_tag="ACUQPX_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HHV9056307.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22959..24026
                     /gene="mxiC"
                     /locus_tag="ACUQPX_001561"
     CDS             22959..24026
                     /gene="mxiC"
                     /locus_tag="ACUQPX_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HHV9056308.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24039..26099
                     /gene="mxiA"
                     /locus_tag="ACUQPX_001562"
                     /gene_synonym="virH"
     CDS             24039..26099
                     /gene="mxiA"
                     /locus_tag="ACUQPX_001562"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HHV9056309.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26112..26513
                     /gene="spa15"
                     /locus_tag="ACUQPX_001563"
                     /gene_synonym="spaK"
     CDS             26112..26513
                     /gene="spa15"
                     /locus_tag="ACUQPX_001563"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HHV9056310.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26517..27809
                     /gene="spaL"
                     /locus_tag="ACUQPX_001564"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26517..27809
                     /gene="spaL"
                     /locus_tag="ACUQPX_001564"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HHV9056311.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27890..28240
                     /gene="spa13"
                     /locus_tag="ACUQPX_001565"
                     /gene_synonym="spaM"
     CDS             27890..28240
                     /gene="spa13"
                     /locus_tag="ACUQPX_001565"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HHV9056312.1"
                     /translation="MYRDVEALDKRIIYFQQLENDLEPVGAQSVSQLFNTRRKIAIVK
                     KHIIQYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLI
                     LDELSQEDMKYGIR"
     gene            28227..29105
                     /gene="spa32"
                     /locus_tag="ACUQPX_001566"
     CDS             28227..29105
                     /gene="spa32"
                     /locus_tag="ACUQPX_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HHV9056313.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29099..29980
                     /gene="spa33"
                     /locus_tag="ACUQPX_001567"
                     /gene_synonym="spaO"
     CDS             29099..29980
                     /gene="spa33"
                     /locus_tag="ACUQPX_001567"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HHV9056314.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29985..30635
                     /gene="spa24"
                     /locus_tag="ACUQPX_001568"
                     /gene_synonym="spaP"
     CDS             29985..30635
                     /gene="spa24"
                     /locus_tag="ACUQPX_001568"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HHV9056315.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            30650..30910
                     /gene="spaQ"
                     /locus_tag="ACUQPX_001569"
     CDS             30650..30910
                     /gene="spaQ"
                     /locus_tag="ACUQPX_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HHV9056316.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30910..31680
                     /gene="spa29"
                     /locus_tag="ACUQPX_001570"
                     /gene_synonym="spaR"
     CDS             30910..31680
                     /gene="spa29"
                     /locus_tag="ACUQPX_001570"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HHV9056317.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31687..32715
                     /gene="spa40"
                     /locus_tag="ACUQPX_001571"
                     /gene_synonym="spaS"
     CDS             31687..32715
                     /gene="spa40"
                     /locus_tag="ACUQPX_001571"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HHV9056318.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGVLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32737..32970
                     /locus_tag="ACUQPX_001572"
     CDS             32737..32970
                     /locus_tag="ACUQPX_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator YmgB/AriR"
                     /protein_id="HHV9056319.1"
                     /translation="MGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKDIIN
                     VLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10485 a         4878 c         6726 g        11486 t
ORIGIN      
        1 ataagctctc ctgaatcagg agagcaggtt accccctgga tctgatttca ggcgttgggt
       61 gtggatcact attgcaccgt tcgtgacact tgttgactgc ctgtgcggca tctggtatca
      121 aggtggcgtg aagccggttc aggttctcat gcggaacgtt ggaacctata tccccccgat
      181 accaaaggcg aaatgtaagt gacaaaatgc aatgaacacg ttctccgtta actgaggagc
      241 atggtgataa ataaaggtaa atgcagaagg tgtagagtct cgcagatttc tgacatcata
      301 gagcctaaca ttagatgcat aacgctacag attgtagaat ctctacaggt gaaatataca
      361 ctggttggcg tttatgtcgc aaaacatgtc attcaggttc atctcaccaa taaggatatg
      421 agtgaagtgg aggataagtg agtctgctgt cagagttttc tggtgtatgt cagtaactgc
      481 gaaccatgtc tgcttgatat gggagcttat ggaggctctc tgtacaaggt tagggagcta
      541 acaaagcagg gataaaaagt ccgcctgatg caggcacggt tcgttaaatc ttttgccatt
      601 ggtaacaaaa tggtgcgatg aatgttcagg ctatctttat cttgatggtg gttcagctgt
      661 ttggtaaaga aatcgctgta acaatagaag aactgcaggc agtactggtc ccacatcgtt
      721 tgctcagtca actggtgaag ttccgtagcg cgtaaattaa tgctttgcat gaaacgttac
      781 tggagtggat gaaagaatac ctgcatatat cattactgcg ttggattacc agtacaacag
      841 attgaatgaa ctggacatgt tgatagacgg cattgaaaag cagcttataa gcatagtgcg
      901 gcagagttaa ttggagagca cttcgccttc tgagtattaa gtaagcatgg tggtacctgc
      961 ggatactatc tattcactgt gctcggtcgg cagccttact gatcaaggag ttgggggagt
     1021 tgataacaaa gttgaaaaaa cagctctcaa ccaaggtggt tattgtaaca atagcaaata
     1081 agctggtgtg acctgtatca caataaccga ttatggacgt aaatacgaca aaaatatgtc
     1141 agtatcaggc ggtattaatt gctgatgctg atggacatta actcttaata aaagggtgga
     1201 tgctgaaagg ttgttatggt gacggacgtg atgacgacgg aaaagagtat gaggttttgg
     1261 atttaaagtt cgttcgggga ataaggaccc ggtcagctaa ttctcatagt agttcgcatt
     1321 gctggatgca atactgtgct gcaattactc gtcgatgtcg gaacaacgga agccttgcaa
     1381 acggggatgt attcatacag gtgttatgtt atatggggtt ttattgcagg gggctatacc
     1441 aagagaatat atgatatctc atcagactga tgtgagggta aatgagaata gagtaaacga
     1501 acaaggttgt tttttggcga ggaagcagat gtatgacaat tcttgtggag ctgccagttt
     1561 actctgtgct gcaaaggaat taggggttga taaaatacca caatacaaag ggtctatgtc
     1621 cgaaatgaca aggaagagtt ctcttgattt ggataatcgt tgtgagcggg atttatactt
     1681 aataactagt ggtaattata atccgagaat acacaaagat aatatcgcgg atgccggtta
     1741 ttctatgcct gataagatag tcatggctac gaggcttctt ggattgaatg cttatgtcgt
     1801 tgaagaaagt aacatttttt cgcaagtaat tagttttatt tatcccgatg caagggactt
     1861 attaattggc atgggatgta atatagtaca tcaacgagat gtgttatctt ctaaccaaag
     1921 agttttggaa gctgttgctg tatcctttat tggagttcct gtcggcctcc actgggtgtt
     1981 gtgtagacct gatggaagct atatggatcc agctgtagga gaaaattatt cttgtttttc
     2041 aactatggaa ttaggtgcaa gaagaagtaa ttctaacttt ataggatata ctaaaatagg
     2101 tatttccata gttataacta atgaggcttt gtaaattgtc gttttttatc tgtgaaggag
     2161 cgtgaatatt ttggggcggg ggggcataac aaatgatatt actgagtaca aatttagcta
     2221 tattgggcgg attagttgtg tgggaagacg aaatgtttct cttgaaataa gtgaactccc
     2281 tcaaggaatg attgtgtgct taaatattta agtgctggta agattgagaa ggctgacata
     2341 ttttgcagag caaacagtga ttgacttgga tgatattttt attttagtca ctcgttgtaa
     2401 ttggagtatg ctttatttta tttgaaaata gatgtatttt atacccgcaa tttaaactgt
     2461 aagccctatg aggggtatgg atattataag ttactctttg actaagcata acttttgggg
     2521 ttagaaaaat aaatctcttg ttaataatta atatgatttt aaaatcaaac ttgtttataa
     2581 ttataaaata atgtaagtcg gtcattttat gggaagatta ggagaataaa acagtaatta
     2641 tatttgagaa atctgctaat atattcttaa agcttttttg atactgtgaa cgaagtctgt
     2701 taatggtgga ttttttgttt atcaccatgt aaaaaaattg agtagtaaaa gcgttgactg
     2761 ctaatcctcg ctgtttttat tgaaacgatt aaaaatacgc tgaatatttt cggccagtca
     2821 ctcatcttca cataaaattt tgaccatcat gccgcatccc ttagttgcac aattcatttt
     2881 caatgatatg aatattacta atttcaccag ataatgttct cgtggtcagc agggtatgag
     2941 ttttcaccgt aattttaagg aaacacagtg cgcggggatt gaataacaga tagccgacgg
     3001 aattacccca ccggctgaaa gtcaagttca acgaagattt ttattatctg aattgggcag
     3061 tttacatcag tgttcgatgt ttatgaagac gatagatggc gagaaattat atcccgaata
     3121 gcttcatcta tttctctctg aactgttttg tttattttag ataatgtaaa tattagagtt
     3181 ctgtttgttt tattctcttt tctttttatg taagtccgtt tgtctttaga aataaataac
     3241 gagtcaactt ttagcgaagg gttttgcttt ctgtttttgc ttttctttat gatgtttaaa
     3301 atttcttttt tatatatgtc cgggggcaaa tttgtatcaa ggtcttttat ttcttccttt
     3361 aatattggta gtgtagaact aagagattca ttagcctttt ctagtccttt ataataattg
     3421 aataatatct tgtagtcatt aaagttaagt tctgacgcga ttggaaatag agatattatt
     3481 tcctgtggaa cgcttgctgc ctgaaaggca cgagtgactt tcgcgcgaga cagattctct
     3541 tttttggcta tgtctttata ggacatccct gatactttca gaaaattaag accaatacca
     3601 agttctcgga tgctatgctc ttttgctgtt tgtatatcgt ttgctagttt tctggcatca
     3661 agagtagata tatattcttt tgaatataga acttcaagat ctgctcctgc atatattgca
     3721 gatgctcttc tacgagtgcc atccagaatt tcaattctac catcaatctc ccttcctatt
     3781 acagggaaga attgttgtag ctttatagtt tttatgatat cggctaatga ttcttctgtt
     3841 agggattctt gatcgcgtac attcgttttt tggttgacga aggttaaatc ttggatcctt
     3901 ttatgaggaa tcttggcttt gataaaggaa actttattac cagaatggag tgtaaactct
     3961 ttcttttggc cttcctttat acttaacaag tcgttgcaca aatccaccat atcacaccct
     4021 gtttattcat attgatttat attgacaaaa gttaaatgca gtgtgagcaa gattgtatgt
     4081 aactgttgat gatagtcaac ggtagtctta aaaattacta ttttaatagc tggattgatg
     4141 caatttatct ttattggata aaagaataat attcttgtgg agctctgatg tgagagctcc
     4201 atagatgaaa aaatgcttat caattactat attgattttg tactattttt gactacagaa
     4261 ataagatctc caatagtaat aatgtgttct aatgcagatt catctatacg tagattgaac
     4321 tcaaaactaa tcatatccag catttcaatc agagaaagag aatcggcaga aagatcttca
     4381 ataaatttag tttcttcaga tagtttgctg tacgctatac catagcaaaa ggccactatt
     4441 gataatattt tttcttttat catattttaa tccttattga tattctttaa tacttttgat
     4501 agggaagtgg ttacatcttt tgctgcttta tatatattat tgttttcagc tgtaatatca
     4561 ttgttatttt ttaaatcaga tatatcatct gtaagtaatt ctgcagaggt atcgtcaatt
     4621 tttgacaata ccttgctgag ggcgctagat acttcttttg ccttttcata gatcgcatga
     4681 tttttatcaa ttgtatctgc gttggtttct gttacgtagt ttattgtatt tgcatctgta
     4741 acttttaatt tgttgtttat tctgtgttct gttgtggatt ttgtaatgtc ctttgacttt
     4801 tcctgtaact ctcgttcttg tgttcctgat ttagttccaa tgagagatat aagattactt
     4861 agggagtttg ttactttttt tgaagcttca aatatcgttt cccgtgttaa atatgtgtct
     4921 ccatttcttg ataaaaaatc aggctgaagt acttttgaat cagtgtttga gttgaattta
     4981 ttgatttgcg aagctactct atgagaaaga gaagcgctac ttttagttgt atctgatgag
     5041 ttatcattac tgcttgtcac tccttccggg tggagagggg gggtggttga agagttctgt
     5101 atgttcagag gcctgtgtcc cagagaaaga gagttatttg cagatctttc ttgactactc
     5161 gtagttaaca ggtctgaacc tattccatcc gaattatctg gagtatggtt gaaaaccttt
     5221 tctaaagctg aattatttgc aaatgtctgg acagtatctg atatagagtg tggtagaacg
     5281 gaagttactt tttgaactag tgctctttgt ggatttagta atgagtttcc gttacgggat
     5341 aatagatcgt ttgtctgaga agtaggagac tgtgcatcat tttctgggtt ctcgtgatag
     5401 ctattgtcat atactctgtt gtcaaaaact ctattatcgt aagttctatt atcattattt
     5461 atatgatagt gaattacagg ttgggatgtt ttctcattgg ctccgggggt tggtccgcca
     5521 tctggcatat cttcgggagg tgtagggagg ccatcttgga taggatgcga atcttttata
     5581 tcttctaaaa tatcattaag gacttttctc aacagctcag gaaacacggc tactgtattt
     5641 aagtctcttg acagtttacc tataccaaat cctttactat caatagtatt tttacgttga
     5701 ttgcgcaaac tatcaaaggc aagttttgcc tgtgcgtcaa ctaaatcatc tagtttgaga
     5761 gatgaaacag tttcattact taactttaat tcagcaagaa tattattctt gataaatgat
     5821 gatacttcag tattgaggag tttttgtccc aatgataaag cttcaccaga tgtgtgttgt
     5881 tgtgctaata gtgcaagtct gtgctcaatg ttttgtcgta attgatggag tttcttattc
     5941 tttgccgacc atggtccaaa ggcagaatct tgggataatt tatcttttat aattagttcc
     6001 gaatactgtg cgacaatttt ttgaagtccc ttttcgtttt caaaattaag accattttga
     6061 tgtactaagg agtacaaaac atttgaaaat tcgagaatct cgcttttggc atacgcattc
     6121 attgagttgg aataaatgct tcttaacaca tcttctttcc ctgaggaaga aaataaaaaa
     6181 tcaagacatt tctgattaaa agacgttgca aagttttctt tttcagacac tgatgctggt
     6241 gtttttagag aagtttgcga actatggata tcggatattt tgctttttaa ctcgctgtat
     6301 tctgttgatg atggtgaagt tgccttatat aagaatgttg gcgcttgagt attatttaca
     6361 ttatgcatgg cgcacctcag aaatggagaa aaagtttatc tgtatctgta catgagctta
     6421 ttgtactact caaaaccttt actaaattat caaaaatact attggcatta ctgtattttt
     6481 gaactaaagt ttgaagatta tttttcattg tttcatcttc ggcagagaat ccggcattcc
     6541 atgcctgata ttttgcatta tctagcacaa cctcgccatt tccacctaga ttatctaagc
     6601 tttttaacat attgtctatt ggggtcatgt ttatattgac aacatatccc ccgttttttt
     6661 cagatacctt gccgattgtt ccacctaatt ctgtaagcca tttatttgct tgttcctgac
     6721 taacagtatt atttgctgga tatagcggtt tatctttata ttttttcttg agttcttcca
     6781 atgcgttttt aagcgagttg acttgtaatt tcacggagtt tccgtcgtta cctccgggag
     6841 agatccagcc ggcaagactg gaaagaacag cgctaaaatc ttgatacatt tgagtatatg
     6901 aactaacggc atgttcatat actttcagat actgttcatt aatatcattg atggagtttg
     6961 caattttagc ccacagttct ctatgagata tcatttgatc tccatcaagc tcggcttctt
     7021 tcggggctga atgtaataat tctcttgcgt ctttattaat tggatattcg ttcctggaaa
     7081 gagtatccaa tagttgagcg gatttattta catccatgct aatctgagat gaatgtaatg
     7141 ctatttcttc tagcgatgtt ttagtcaacg ttttttgtga aagctctttc tttaatgcct
     7201 gatttgttgt tctgatatta tgaagggtgt cgttgagcat agtaagggaa cttacaggat
     7261 gaaaactggt cgttgttttt atatcagaat taactgtttc ggttgatgaa ccgttggtat
     7321 tgtttggact gaatgatgag gtggaaatac tattagtcag agttgttata ttcatggtta
     7381 tttccttaca ggaagagcca tatattatta tggctcttgg atatattaag ctcgaatgtt
     7441 accagcaatc tgactggctg tcgaattctt tgattgggtg atgctgtcaa ttatattcaa
     7501 taatttttgt attaattgat ttgtcgcttg ggatgcttct ttagatactt gggatgcttc
     7561 ctctgcttgt ttactgctgg cctgactgat tagttgttct tcttcttcaa gagcagatgc
     7621 ataacgccct cctgatgtgg atatattacc agcaacggct gatgtgtcca ttgttgcacg
     7681 gccaatgttt tgtttttgct gcgcagaaag ggtattgagc tcgtaagttc ttctctgggt
     7741 gtcaatttta tcctgcaaag aaatatcggg agaactaaga gaagttttat gttcagttga
     7801 cagggatata ttatctggcg ccagtttatt tttacctaaa aattttgtgc tagagttagt
     7861 ttgtggtgag gtgatatttg tatctatttg tttatttaac cctaatttag aacctgcaag
     7921 ctctttttca agagattgag cagtggcaag gttctttctt aaggctcctt tttggtcgct
     7981 aatccctgaa tgcgtttttt tggcaccgat acccgttata cctacttgtg tgactgctcc
     8041 agtaatgctt gatgataggg ctgccaggcc ttgccgaaca atgttctctg cagctgattt
     8101 tgtagcatcg aacgcaatca atgacaattg agagcccaat ttagtttctg cagtgcggag
     8161 agcagaaagt agaacggcta cactaataat taaagaaaca gcattagaag aaagactgga
     8221 aatatccaga gtgttctctg gagtaagggt gtgactcatc tctgaaagat ctttcatctt
     8281 tttgtcagta aggtgagcaa tgatttctga atcatgctga acctgctctg ataactttaa
     8341 aagttgatca tcatttaatg tggttgttaa tacgggattt ttaccgacat taagtggaat
     8401 atgcgctgca atctgctgat aattttgtga tttttgtgtt tcggaagaac tttgagtttg
     8461 ttttgtggat atatctgtat ataaaatctg ggttgatttt gtgttttgaa tttccataac
     8521 attctcctta tttgtatcaa gcagtagttt gttgcaaaat tgcttttgca acatcagttc
     8581 tattagcctg agagttggac attgaggcta atagatctgc aattacttcc tgcaattggc
     8641 cgaatttttc tattgcttca ctgatatatt ttgacagttg ttcaacttga tatttcgata
     8701 atgtcaggtc tgctagattt gtcgacgcgc tgttctggaa aacagcagaa gcgacacttc
     8761 ctcccgcttg tgtggcagag ttaacacttt ctcctaataa aactgcttgg tttaaatggg
     8821 tggaaataat ttgtttggat attacttcat cacccgctgc accaagaaat ttatttaatc
     8881 tggcaacagc attagtgatt aaatcgtcca gttgagaaga aaaactcttg agaaactttg
     8941 gtataaggtc tgtgagggtt ttacctatta ttttgccaat attctctgca agttttgctg
     9001 ctgcctgttt accaacagtg gctacgagaa cgactgctgc aactaggaca agagcgcctg
     9061 cgattgcccc cagaatagag ccaatcattt tggctttttt cgagtcgacg cccaagcctt
     9121 cgagcatttt tgtaaatgca tctgaaagga gtttgattaa gggttcaatg actgctttca
     9181 tgatcggatt cagggcttgt tccatgaagg aattgccggt cgctgcttgt actatagcat
     9241 ccgtaaccat aagagctaaa ccaacagctg ccagtgctag agaggctcct ccagaaaaag
     9301 ctgctgcaac aacactaacg atagttaaaa gtgccccaag tattttccca acacaaccca
     9361 ttactctgtt gagttcttct gctttacgta cttcagcagc atactcatca gattttctct
     9421 ccatttcagt ttttcttgat tcttggagag actggaatag agccagatca ttttttaaag
     9481 attcttcatt attttttcca actagttgaa taaaggttgc catcaattga gtaacactgg
     9541 caagtccggt taatgatttc tgctgggttg atagctgttc agcagatgct gtgtttgaaa
     9601 atgcagaaaa agagtctatt tctttttcga gttgcattga tttatctgta agtttgctat
     9661 gaattgacag ggttttctgc tcaatcaatg tcctgtcttt aactgctgcg tcttttttga
     9721 tagtgagttg tatttcttcc cggcttaatt ttttcttttc tggtgaatct ggggcgagtt
     9781 cggataatct tgtttgaatt tggttaattt tattttctag gtcttttatt ttagaatctg
     9841 cgttttttag tttattaatt tgtttttcat agtctctggt tagtccttca gtttcagata
     9901 gaagagtatt aattttatcg gagaattcta ggtttttttg ctgtcttgcc tgttgctgag
     9961 acttccaagc agtaatttta tttgttaatg cagttaaaga tttttcaccg agtatttgaa
    10021 taaggtttcc aattaaaagc gttagttggg aacttgcatt taatgacttt ggtgctttaa
    10081 gttcagggat taatatattt gaagttgagt gtgcattagt tgtattaata tttttgttag
    10141 cagtaagatc agcaattgta agagaaaata atttgttagc tgcatcattt gcagcctgga
    10201 tagtattgtc tccaagctca gtggaagtca atattttgtc aagagacaaa ccagtggttg
    10261 tggtacttac attatgcata ataattactc cttgatatcc tgaattgcgt ccaagtatga
    10321 ttgtgctttt atttttaatt tttcatcatt gctgtgttga attacgagtt cgaagcactc
    10381 tttggctttc aagggggctt tcaaccgaag ctggcattgt ccagtatgga ataccggtgt
    10441 atagtcattt tttcctaatg caaaagcgac agcataaagg tctgctgctt gttggaactg
    10501 ttcttttatc tgataaatag ctgcgagtcc cataatgtag tctacattgt aaaagtcgta
    10561 tatacataaa aatctgaaga aaacttcagc ttcctctatt cttcctttgt tgtaaaagtc
    10621 ataagcatat gaataaatgt catccatcat atcatcagga attgcattaa tatctttcag
    10681 tgtagcgcca gagttaattg catcaattac tgcagtagag atgctttcat tttcagtgat
    10741 atttaaagac ataaggtctc ctttttgtct gctttacttt aaaaagtata agatttaata
    10801 taaacagatt taatttgtat tgctttgacg gtatacggcc tttagaacct ctgcgcttat
    10861 ttctatttgt aaaacttttg ttgattcatt aaatatttta tcgtagacgg aactggatac
    10921 tagatttgta agatccttca tttgtgtgtt aaacaaatcc atgtcactgc tatagcgttt
    10981 taaaatcttt gctctgaggc cagatgaggc aatctcgttt agtgctgtat gactgataga
    11041 tgtttgtgct gatttttcat caagttttat atccgggaat tttttttcaa tagctgcaaa
    11101 tatgtttttt ttgttatctg aggtcaaaac aactccattg tgtatggcca tttcatctat
    11161 acttctgttt atcattgaag agacataaga ggttcgctct tggctcatcc aacaaaatag
    11221 ttgattcccc gaattactat cttttatttt gtctatgacg ttgtccctga ttttatttat
    11281 attcaccggg catccaaaat tcttctgagg aaagcgttgt tttaagttaa aaacagataa
    11341 tattttcttt acaagcttat tgtaacttaa cgaattaaat gatgggcgag gaatcgcgaa
    11401 ttcaacctgt ggaagtattt tgtttagaat ttgcatgata ccccctatat gttagttcac
    11461 ttctgaagtg atgtttgcaa tggttagttt aatacaaaat aaaaattcat acaaagattc
    11521 aatgagttca tcaggtgaaa gatatttttt gtagtgtttc ttggtcaaaa cacattgatg
    11581 agtatcaggg agaagacaga tagaagaacc ataatctcta ctcaaagagt agttaaggat
    11641 gagtatgtgt tctggattaa taatattttc tgtaaaaagt tcatgttcat taagaagtcc
    11701 ggtcaaaaag atgtattcat cattatctgt tatacaaaca ggtatttgtt cttcgagcaa
    11761 tataaaggct tgatttttat cgttaaaatc aagctttgca cctgttattt cataaagttt
    11821 atcatatagt ttgcgacaca tttctatggc cttctatata ttagaatgag agttattcaa
    11881 taaaagctta ttgatctgta ataagtcgtg aatgtaaagt ttggttccaa gatctggcga
    11941 tttaagagaa ttgtaataat caatagtttt tattgacttt tcgataattt cttctttttt
    12001 atctgttgct ccggttttta attcgtttaa tacacgagac atattgctgg gtgctgattt
    12061 tactaagtat gagtggcaag cgttgatgac cgcatcaaag tcttctgaag atacattttc
    12121 agtaggatgt ttgtaaagtg aaattgataa aggcttgaat gtttttgggg tgtggataag
    12181 gttaatttta ttgaaacttt taagatagct acgtcgtaat tgagtcaatc tggaaagatc
    12241 aaaatcagat tcaatatact tcattatctc gttataagac tgattgagac tttcaatgcg
    12301 ttgcctaact ttatttgcat acgcgtatac attgttaggt gtagcaacca atttaacgtc
    12361 agggagtgga aagtatattt cagaatttcc tgctttaaga acattaagtg ccataccagc
    12421 acagttttca gatttactga ttaatttata atttgccaat tgtctgaaag catctgcttt
    12481 cttttggcat ataaattttg acatgtttgt ttcatcaagg ccaaaaaggt tatagctgat
    12541 tttaccatcc tttgttttat tatcacctga taatggaatg tatactttat cagcactgac
    12601 cccccaataa ccataggggt cttttttttg attagctctg ggaaaaaata tttttttgtt
    12661 gttattttga tcttgttttg aatttaataa gtttctaacc tcttggcctg catcaagtct
    12721 ttcggctgtt tggtctgaaa tcatatttag tttgtcttgt ttataggtat cgacactgta
    12781 acctgagcgt ttttcaaaaa ggtagtttat ttctgtatct tttgtaacat tcccgccggg
    12841 ataccagctg agataggtat gagttggctc ctgtagtaaa ttgtttttta tactgatact
    12901 ggtatgccct aaatgggtta tgcctctatt tccccagcaa cgaaaaacta aatgtctttc
    12961 aagatttttg gctgtaccga tgccatgaaa acgctctgcg gtattcaaca ttaatttgat
    13021 gtctttacct gtaaaagaat gttgaattga atttttgact gcttcatagg cggcaaacgc
    13081 actttctgca gatttttcgc tagctaatgt cttgaggaaa agcaataagg atttttgatt
    13141 cgctagtcga taatttttta gttgttcatt taatatctgt gaatttatct ttgcattaat
    13201 agtgcttaat gttctgaaaa agagatcctt taggttgaat ttctgtgcta ccaacatagg
    13261 tccatgctct gtatcttcaa cgcgtatagg cccttttgta ttgcttgcgt caatgaaatt
    13321 gctaattttg agaatcatac tttattaact ctccattacg tggtgattgt ggggatgtaa
    13381 aatgtatttg atatataata tattcaatta ataaatttag aaacttgagc ctgttaacat
    13441 aatcaaattt tcttttgttg tacataatat gtacctcgtg agcatatgta gtgctcgttt
    13501 catcatgaaa tcccacaagt taaagtgtct gatatatcag gctcggagtg ttatagaaaa
    13561 agagagaacc ctgttgaata agtatctttt ttgttgtaaa acgaggtaca aaactgcaat
    13621 caatgcccca tttatgacgg aggataatta aatgcacata actaatttgg gattgcatca
    13681 ggtttcattt caaagcggag attcctataa aggcgcagaa gaaaccggaa acccaaaagg
    13741 tgtaagcgtg atttcatatc aaagagttaa aaatggagag agaaataagg gggtcgaagc
    13801 gcttaatcga ttatatttac agaaccaaac aagcttaaca gggaaaggcc ttttgtttgc
    13861 gcgtgacaga gcagccgttt tttacgaggc aataaagttg gcaggtggtg atacgtcaaa
    13921 aattaaagcc atgatggaac aattagatac ctataaactt ggtgaagttg ataaaagaaa
    13981 tattaatgag cttaataaag taataagtga agagataaga gcacagctag gcattaaaaa
    14041 taaaaaggaa ttgcaatcgg aaataaaaca aatatttact aaatatttaa ataataagaa
    14101 ttgggaaccg atcaacaaaa atattaatta tcatgggaaa aattatggtt ttcaattaac
    14161 acccgcctct catatgaaga tagggaataa aaatatattt gtcaaagagt ataatggaaa
    14221 aggaatttgt tgtgcttcta cgagagagag tcagcatatt gcgaatatgt ggctttcaaa
    14281 agtggtggat gacgagggga aggaaatttt ttcgggtatc agacatggag ttatatcagc
    14341 ctatggattg aaaaaaaact catctgaaag agctgttgct gctcgtaata aagctgagga
    14401 gttagcaagc gcagcattat atagcagacg agaattatta tcacaggctt tgtctggtaa
    14461 aacagtagat ttaaagattg tttcgacttc tcttctgacg ccgaccagtt taactggggg
    14521 agaggagagt atgttaaaag atcaggtaaa ggcattaaaa gggttaaact ctaaaagggg
    14581 ggaaccaacg aaattattaa ttcggaatag tgatggtctt ctgcaagaag taaacgttaa
    14641 tctaaaagtg gtgacattta actttggtgt aaatgaatta gcgctaaaaa tgggcttagg
    14701 ctggaggaat gttgacaagc ttaatggtga atcaatatgt tctttgttgg gggataattt
    14761 cctcaaaaat ggtgtgattg gaggttgggc tgcagaagct atagaaaaaa atccaccatg
    14821 taagaatgat gtgatatatc tggctaacca gataaaagat attgtaaaca agaagttaca
    14881 aaaaaatgat aatggagagc cgtataaatt gtcacaaaga atggctctat tggcttatac
    14941 tataggtgct gtaccttgct ggaattgtaa gagtgggaag gacagaacag gtatgcagga
    15001 tgctgaaatt aaaagagaaa taataagaaa acatgataca gggcagtttt ctcaattaaa
    15061 tagtaaatta tcctcagaag agaaaagatt attttctact attctaatga atagtggtaa
    15121 tatggaaatc caagagatga atacgggtgt gcctggaaat aaagttatga aaaaattacc
    15181 gctatcttcg ttagagctgt cttatgctga aagaataggg gaccccaaaa tatggaatat
    15241 ggtgaaaggg tattcgtcat ttgtataaga ggaatatatg gaagatttag cagatgttat
    15301 ttgccgagcc ttgggtatcc cttcaattga taccgatgat caagcaatca tgcttgatga
    15361 tgatgtgtta atatatattg aaaaagaagg agattcaata aatttattat gccctttttg
    15421 tgcattgcct gaaaatataa atgatcttat atatgcgttg agtctaaatt actcagaaaa
    15481 gatatgttta gctactgatg atgaaggtgg aagtctaatt gcgcgattag atcttaccgg
    15541 gataaatgaa ttcgaggata tatatgttaa tacagaatat tatatttcgc gtgtgcgttg
    15601 gctgaaggat gaatttgcgc gaagaatgaa ggggtattaa tgtcccgttt cgtatttatt
    15661 ttgctgtttt ttatcccata tttagggaga gctgattgtt gggataaggc tggtgaaagg
    15721 tacaacattc catcgagttt gttaaaagcg attgcggaaa aagaatccag atttaataaa
    15781 tctgcagtca atgttaataa caatggaagt aaagattatg gtataatgca aataaatgac
    15841 tttcattcta aaagacttag agaaatggga tatactgagg aaatgctaat tagtcatcca
    15901 tgtctttctg tacattatgc agcaaagtta ttaaatgagt ttatgctgat gtatggaaga
    15961 gggtgggagg ctgttggtgc ctataatgca ggaacatctc cgaaaaagaa aaaagaaaga
    16021 ttaaagtacg cagaggatat ctataaacgc tatttgcgga tagcagcaga gagcaagcag
    16081 aataatcgaa ggatataaga ggatttatgt ctgaggcaaa gaactcaaat cttgcaccat
    16141 tcaggttatt agtgaaactc acaaacggtg tcggggatga gtttccgtta tattatggga
    16201 ataacttaat tgttctcgga cggacgatag aaacacttga gtttggaaac gataatttcc
    16261 cagaaaatat tattccagta acagatagta aatcggatgg tataatttat ttggccataa
    16321 gcaaagataa tatctgtcaa tttagtgatg aaaaaggtga acaaattgat atcaattcac
    16381 aatttaactc atttgaatat gacggagttt cttttcaact taaaaatatg atggaggata
    16441 aatcgagagt tcatatacta aacggaatgt ataataaacc ctcggtgttt tttttctttg
    16501 ctgttattgt tgtgttaatt ataatttttt ccctctcttt aaagaaagat gaagtaaaag
    16561 aaattgctga aattatagat gataagagat atggaatagt caacacagga cagtgcaatt
    16621 atattcttgc cgaaacacaa aatgatgcgg tatgggcatc tgtagcctta aataaaaccg
    16681 gatttaccaa atgcagatat attctggtgt ccaacaaaga aataaataga attcaacaat
    16741 atatcaatca acgattccca tatatcaatt tgtatgtgtt gaatttagtg agtgataaag
    16801 cggaattact ggtattttta agtaaagaaa gaaatagttc aaaagatacc gaacttgaga
    16861 aattgaaaaa tgctctgatt gttgaatttc cgtatatcaa aaatattaaa ttcaactatc
    16921 ttagcgatca taatgcaagg gaggatgcta aaggtatttt cacaaaggta aatgtccagt
    16981 ataaagagat ttgtgaagct aataaagtta catattctgt cagagaagaa ctgacagatg
    17041 aaaagcttga gctcattaat agactgattt ctgatcataa aaatgtatat ggagatcagt
    17101 atattgagtt ttctgtactt ttgatagatg atgattttaa aggtaaatca tatcttaaca
    17161 gcaaagacag ttatgtaatg ttgaatgata aacactggtt ttttttagat aaaaataagt
    17221 gaggataaaa tgagtgttac agtaccggat aaagattgga ctctgagttc attatctgaa
    17281 acttttgatg atggaactca aacattacaa ggtgaactaa cattggcact agatgcatta
    17341 gctagaaatc cttcgaatcc acagttgctg gctgaatacc aaagtaaatt atctgaatat
    17401 acattatata ggaacgcgca atctaatact gtgaaagtga ttaaggatgt tgatgctgca
    17461 attattcaaa actacagata atagggagca ttcatgaatt acatttatcc agtcaatcag
    17521 gttgatatta tcaaagccag tgattttcaa tctcaagaga tatcaagtct ggaagacgtc
    17581 gtgtcggcta aatatagtga tattaagatg gatacagata ttcaagtatc acaaataatg
    17641 gagatggtag gcaatccaga atcattaaat ccagaatctt tggccaagtt acagacgacg
    17701 ctctcaaatt attcaatagg agtatcatta gctggcacgt tagcaagaaa aacagtttcg
    17761 gctgttgaaa ctttattaaa gtcttaattt atatgattag gtataaaggt tttattttat
    17821 tcttgttgct gatgttgatt ggatgtgagc aacgtgaaga gttaatttct aatttatctc
    17881 aaagacaggc aaatgaaata atatctgtgc tagaacgcca taatattact gctagaaaag
    17941 ttgatggagg taaacagggg atctcggtac aagtcgaaaa ggggacattt gcatcggcag
    18001 ttgatttgat gcgcatgtac gatttgccaa atccggagag agttgatatc tcacaaatgt
    18061 ttcctacaga ttcattagtg tcttctccaa gagctgaaaa ggcccgttta tatagtgcta
    18121 ttgagcaacg gctggaacag tctttagttt ctattggtgg tgttatttcg gcaaaaatac
    18181 atgttagcta tgatcttgaa gaaaaaaata tatcttcaaa accgatgcat atatcagtaa
    18241 tcgctatata tgactcaccg aaagagtctg aactattagt tagtaatatt aagcgatttt
    18301 tgaaaaacac cttttctgag gttaagtatg aaaatatatc tgtcatatta actccgaaag
    18361 aagaatatgt ttatacaaat gtacaacctg ttaaggaaat taaatcggaa tttttaacaa
    18421 atgaagtaat atatgtattt ctcgggatgg ctgtactagt tgtcactctt ttggtatggg
    18481 cattcaaaac agggtggttc aagagaaaca aaatatgata agaatggatg gaatttataa
    18541 aaaatatctt tcaataattt ttgatccagc gttctatata aatagaaatc ggttgaattt
    18601 gccttctgaa ctgttagaaa atggcgtaat cagaagtgag attaataatc tcataattaa
    18661 taaatatgat ctaaattgcg atattgaacc tttaagcggg gtaaccgcta tgtttgttgc
    18721 caactggaat ttacttccag ctgttgctta ttttatcggt agtcaagagt ctcggttaat
    18781 taatcatagt gagatggtta ttagttatta tgggggcaaa atcagtaagc aaggggaggc
    18841 cgctattcga agtggttttt ggcatcttat tgcgtggaag gaaaatatat ctgttggtat
    18901 atatgaaaga ataaacttat tatttaatcc aattgctttg gagggaaatt atacgcctgt
    18961 agaaagaaat ttgtctcgat taaatgaagg tatgcaatat gcaaaaaggc actttaccgg
    19021 tatccagaca tcatgcctat gatggtgttg ttataaagag aattgaaaag gaattatgta
    19081 aaaccataaa agatagagac actgagagta agaagaaagc aatttgtgtt ataaaggatg
    19141 ctacgaaaaa agctgaatca ctgcgtatag atgctgtatg tgatggttat caaattggta
    19201 tacagactgc ttttgagcat attattgact atatttgtga atggaagtta aagcaaaatg
    19261 aaaacagaag aaatattgag gactatataa ctagtttgct ttcagaaaat ttacatgatg
    19321 agaggatcat taacacttta ttggagcagt ggcttagttc gttgcgtaat acagtaacag
    19381 aattaaaagt ggttttgccc aagtgtaatt tagcactgag gaaaaagtta gaacttgatc
    19441 tccataaata tcgctcagat gtcaagatta ttttaaaata ttctgaaggg aataattata
    19501 tattctgtag tggtaatcaa gtagtggagt ttagtcctca agatgtaata tcaggtgtta
    19561 aaatagaact tgcggaaaaa ttaacaaaaa atgataaaaa atatttcaaa gagttagcac
    19621 ataagaaact aagacaaatt gcagaagact tgttaaaaga gaatcctgtt aatgattaat
    19681 caaataaatg caagcaatgc tctgcagcaa agattaaata gtgaagaatt tgttaattta
    19741 aatgagagat tatcatcttc gcaatcattt gatgaggata taatctatga aataatgcaa
    19801 tatttctcac agagtgaatt aaattccata gataatgatg aactgcacaa taagattgaa
    19861 caattattca attcacgttt tccttatctt acagctgcac aaaaaagctc acttcttaat
    19921 aaactcatag atgcaaatca atatgtcgat ttgcatgagg gattttatgc atcattgtcc
    19981 atatataaca atattgattt ctatattaag actacaacct tcgattcatt aattagtgtc
    20041 tttgacgcag ggagagaggc agatgattcg acatggtagt aataagttga aaatatttat
    20101 tttaagtata ttgctattaa cactgagtgg gtgtgcttta aagtcatcat ctaattctga
    20161 aaaagaatgg catattgttc ctgtaagtaa ggattatttt tctattccaa atgatttatt
    20221 atggtcgttt aatacaacca ataaaagtat aaatgtttac tctaaatgta ttagtggtaa
    20281 ggcggtttat agttttaatg caggtaaatt catgggcaac tttaatgtta aggaagtaga
    20341 tgggtgcttc atggatgcac aaaagatagc tatagataaa ctattttcta tgctgaaaga
    20401 cggggttgtt ttaaaaggta ataagataaa tgataccatc cttatagaga aggatgggga
    20461 agttaaatta aaattaattc gagggatata attgtattgt gagtaaatat aaaggtctaa
    20521 atacaagtaa tatgttttac atttactcta gtggacatga accagttaac gttgagcttg
    20581 taaaagataa agaacgtaac ataattgagc tggctccagc atggaagggt ttttttttgt
    20641 ccgaaatcaa aatataaaat tcagtgataa tgtgaactat cattatcgct ttaacattaa
    20701 tagttgtgca aaatttcttg ctttttggga ctatttcagt ggtgctctag tagagcatag
    20761 ccatgctgaa aaatgtattc atttctatca tgagaatgat cttcgggata gttgtaatac
    20821 cgaatcgatg cttgataagt taatgttaag attcatcttt tcatctgacc agaatgtaag
    20881 taatgcactg gctatgatac gaatgactga gtcatatcat ttggttcttt atcttctgag
    20941 aactattgag aaagaaaagg aggtaagaat aaagagtctg acagagcatt atggtgtgtc
    21001 ggaggcttat tttagatctt tatgtagaaa agctttaggg gcaaaagtca aagaacaact
    21061 gaacacttgg cggttagtaa atggtttatt agatgtattt ttgcacaatc aaacaatcac
    21121 ttctgctgcc atgaataatg ggtatgcatc aacatcccat ttttcaaatg agataaaaac
    21181 acgactaggc ttttctgcaa gagaattatc gaatattact tttttagtga aaaaaataaa
    21241 tgaaaaaatt taatattaaa tctttgactc tcttgattgt attgttacca ttgatagtta
    21301 atgctaataa tattgactct catttattag aacaaaatga catagcgaaa tatgttgctc
    21361 aaagtgatac ggtaggatct ttttttgaac gtttttctgc gttattaaat tatcctattg
    21421 ttgttagcaa acaagctgca aaaaagagaa tatctgggga gtttgattta tctaatccag
    21481 aagaaatgct tgaaaaactg acattacttg tgggactcat atggtataaa gatggaaatg
    21541 cattatacat atatgactct ggagaattaa taagcaaagt tattttgctg gagaatatat
    21601 cacttaatta tcttattcag tatctgaagg atgctaacct ttatgatcac agatatccta
    21661 taagaggaaa cattagtgat aaaacattct atatatctgg tcctccggca ttggttgaac
    21721 tggtagctaa tacagcaact ttgcttgata agcaggtttc ttctattggg acagataaag
    21781 ttaattttgg ggttataaaa ttaaaaaata catttgttag tgacagaact tataatatga
    21841 gaggggagga tattgtcatc ccaggggtgg ctactgttgt agaacgttta ttaaataatg
    21901 gtaaggctct gagtaacaga caagccaaaa atgatccgat gccacccttt aatattacac
    21961 agaaagtatc tgaggatagt aatgactttt catttagttc agttaccaat agttctatat
    22021 tagaggatgt atcacttatt gcatatccag aaaccaattc tatattagtc aaaggtaatg
    22081 atcagcaaat acagattatt cgcgatatta tcactcagtt agatgtagcc aaacgacata
    22141 tagaattatc attatggata atcgatattg ataaatctga attaaataac cttggcgtta
    22201 attggcaagg aacggcttca tttggtgatt cgtttggtgc gtcatttaat atgagctcat
    22261 cagcatcgat aagcactttg gacggcaata agtttattgc ttcagttatg gcattaaatc
    22321 agaaaaagaa agcaaatgta gtttcaaggc cggtgatttt gacacaagag aatattccgg
    22381 ctatatttga taataacagg actttttatg tgtcattagt tggtgagcgc aattcttcgt
    22441 tggagcatgt tacgtatgga acattgataa atgttatacc tcgcttttca tctcggggac
    22501 aaattgagat gtctttaacg atagaggatg gaacaggaaa tagtcagtca aattacaatt
    22561 ataataatga aaatacatct gtccttccag aagttggaag gaccaaaatc agtacaatag
    22621 cgagagttcc acaaggcaaa agtttgttga taggtggtta tacgcatgaa acgaactcta
    22681 acgaaatagt atctattcct tttctctcct ctattcctgt aatcggtaat gtgttcaagt
    22741 ataaaacaag taatatcagt aatattgtaa gggtttttct gattcaaccc cgtgagataa
    22801 aggagtcaag ttattataat actgcagaat ataagagcct catatctgaa agagaaatac
    22861 aaaagactac acaaataatc ccatctgaaa ctactttgct ggaagacgaa aaatcattgg
    22921 tttcatactt aaattactaa ctataaagta ggtgatgtat gcttgatgtt aaaaatacag
    22981 gagtttttag ctctgcattc attgataagt tgaatgcaat gacaaattca gatgatggag
    23041 atgagactgc tgatgcagag cttgattctg gcttggctaa tagcaagtat attgactcat
    23101 ctgatgagat ggcttccgct ctttcgtcat ttataaacag aagagacctt gagaaactga
    23161 aaggaacaaa tagtgatagt caggaacgta ttttagatgg ggaagaagat gaaattaatc
    23221 acaagatttt tgatttaaag agaactttaa aagataacct tcctctagat caggatttta
    23281 tagacagact aaagagatat tttaaagatc caagtgatca agtcttagca ttaagggaac
    23341 ttttgaatga aaaagatctt actgctgaac aagtcgaatc attaactaaa attattaatg
    23401 agataatatc aggtagtgaa aaaagtgtta atgctggaat aaattcagct atacaggcta
    23461 aattatttgg caacaaaatg aaacttgaac cacagctttt gcgtgcatgt tatcgtggtt
    23521 ttatcatggg gaacatatca acaacagatc agtatataga atggcttggt aattttggtt
    23581 ttaatcacag acatacaatt gtgaattttg tagagcagtc actgattgta gacatggatt
    23641 ctgagaaacc gagctgtaat gcttatgagt ttggttttgt gttatctaaa ttaattgcaa
    23701 ttaagatgat tagaacttca gatgtaattt ttatgaagaa actggaatcc tcaagcttgc
    23761 taaaagatgg cagtttaagt gcagagcagc tattgctaac tttattatat atttttcaat
    23821 atccaagtga aagtgagcaa attcttactt ctgttataga cgtatcacga gccagtcatg
    23881 aggattctgt agtgtatcaa acatatctat cttctgttaa tgaaagtcct catgatatat
    23941 ttaaaagtga aagtgaaaga gaaattgcga tcaatattct acgagagctt gtcacaagtg
    24001 catacaagaa agagctttct agataacagg agatgaaagt gatccagtct tttcttaagc
    24061 aagtaagtac taagcctgaa ttaattatat tagtgcttat ggttatgatc attgctatgt
    24121 tgatcatacc attacctaca taccttgttg attttttaat agggcttaat attgtacttg
    24181 ctattctcgt ttttatggga tcattttata ttgaaagaat attgagtttt tctacattcc
    24241 cttcggtttt gttgataacg acattatttc gtctcgcttt atcaataagt actagtcgac
    24301 taattttagt tgatgcggat gcgggtaaaa ttattactac atttggccag tttgtcattg
    24361 gtgatagtct ggctgtaggt tttgttattt tttcaatagt aactgttgtg cagttcattg
    24421 ttataacaaa aggatctgaa agggttgcgg aggtagctgc tcgcttttct cttgacggta
    24481 tgccggggaa acaaatgagc attgatgcgg atttaaaggc tggaattatt gatgccgcag
    24541 gagctaaaga aagacgcagt attctggaac gtgagagtca attgtatggt tcatttgatg
    24601 gagcaatgaa gttcattaaa ggtgatgcaa ttgctggcat cattattatt tttgtcaatt
    24661 taataggagg tatttctgtt ggtatgagtc aacatggaat gtctctatct ggtgcattat
    24721 ctacttatac catactaact attggtgacg gattggtctc tcagattcct gctctgttaa
    24781 tttctataag tgcaggattt atcgttacaa gagttaatgg tgatagcgat aatatgggac
    24841 gtaacataat gtcccagata tttgggaatc cttttgttct cattgttaca tcagctcttg
    24901 ctttggcgat agggatgttg ccaggttttc cgttttttgt ttttttcctg atagcagtta
    24961 ctttgacggc tttattctat tataaaaagg tcgtagaaaa agaaaaaagt ctgtctgagt
    25021 ctgattctag tggctatact ggtacatttg atattgataa tagccatgac tcatctttgg
    25081 caatgataga aaatctggat gctattagtt cagaaaccgt tcctttaatt ttattatttg
    25141 ccgaaaataa gataagtgca aatgatatgg aaggtcttat tgaaaggata agaagtcagt
    25201 tctttattga ttatggtgtc aggcttccaa ctattttata tagaacaagc aatgagctta
    25261 aggtcgatga tattgttttg ttaataaatg aggtgcgtgc cgatagtttt aatatatact
    25321 ttgacaaagt gtgtattaca gatgaaaatg gagatataga tgctctaggt attcctgttg
    25381 tttcaacttc atataatgaa cgtgttattt cttgggtaga cgtttcatat acagaaaatc
    25441 taactaatat tgatgctaaa attaaaagtg ctcaagatga attttatcac cagttgtcac
    25501 aagctttatt aaacaacata aatgagattt ttggtataca agaaacaaaa aatatgttag
    25561 atcagtttga aaatcggtat cctgatctat taaaggaagt cttccgacat gtgactatac
    25621 agagaatttc tgaggtatta caaagattgc ttggagaaaa tatttctgtt cgcaatttaa
    25681 aacttattat ggagtctttg gcgctttggg ctcccagaga aaaagatgtt ataacattag
    25741 ttgaacatgt ccgtgcatca ctttctaggt atatttgtag taaaatagct gtttctggtg
    25801 agattaaagt tgtgatgctt tccggatata ttgaggatgc aataagaaaa gggataaggc
    25861 aaacctctgg tggctctttc ttgaatatgg atatagaggt ttcggatgag gtaatggaaa
    25921 ctttagcaca tgctttgaga gaattgagaa atgcaaaaaa aaatttcgtt cttttggtat
    25981 cagtagatat acgtaggttt gttaaaagac ttatagataa cagatttaag agtatactcg
    26041 ttatatcgta tgctgagatt gatgaagcat ataccattaa tgtattaaag actatttagt
    26101 gaggtttaaa tatgagtaac attaatttag ttcaattagt tagagatagt cttttcacga
    26161 ttggttgtcc tccatcaatt attacagatt tagacagcca ttcagcgatt accatttctt
    26221 tggatagtat gcctgctatt aatatagctc ttgtcaatga gcaggttatg ttatgggcta
    26281 attttgatgc accaagtgat gtaaaacttc agtcatctgc ttataacata ttaaatttaa
    26341 tgttaatgaa ctttagttat agcattaatg agctggtaga gcttcatcgt tctgatgagt
    26401 acttacagtt aagagttgtt attaaagatg actacgttca cgatgggatt gtttttgcag
    26461 agattctgca tgagttttat cagaggatgg aaatcttaaa tgaggtctta taatcaatga
    26521 gctatataaa attgctcact caattatctt ttcctaatag aatctcgggg ccaatcttgg
    26581 aaacaagtct tagcgatgtt tcgattggtg agatttgtaa cattcaggct ggaattgaaa
    26641 gtaatgaaat tattgcaaga gctcaggttg taggatttca tgatgaaaaa acaatattaa
    26701 gcttgattgg aaattctcgt ggactttcac ggcaaacgtt gattaagccc actgcccagt
    26761 ttcttcatac gcaagtcggc cgtggattat tgggagcagt agtcaatcct ttaggggagg
    26821 ttactgataa gtttgctgtt acggataaca gtgaaattct ttatcgacct gtagataatg
    26881 ctcctccgct atatagtgaa agggctgcaa ttgagaagcc ttttttaaca ggtgttaagg
    26941 ttattgattc tttactcacg tgtggtgaag gacagcgaat ggggattttt gcgtcagctg
    27001 gttgtggcaa aacttttctc atgaatatgc tcattgaaca tagtggtgct gatatatatg
    27061 ttattgggtt aattggtgag cgaggtcgag aggttacaga aacggttgat tatttgaaaa
    27121 actctgagaa aaaaagcagg tgtgttttag tatatgcaac ttcggattac tcttcggttg
    27181 atcgttgtaa tgctgcatat atagccactg ctatagccga attttttagg actgaaggac
    27241 ataaagtagc gctttttatt gattcattaa caaggtatgc cagagcatta cgtgatgtgg
    27301 ccttagccgc tggagaatca cctgccagaa gaggctatcc ggtttcggtt tttgatagct
    27361 tacccagact tcttgaaagg ccaggaaagt taaaggcagg tggctctatt actgcatttt
    27421 acactgttct tttggaggat gatgattttg ctgatccatt agctgaagag gtaagatcca
    27481 ttttagatgg acatatatat ttgagcagaa atctagccca aaaaggacaa tttcctgcaa
    27541 ttgattcctt aaaaagtata agcagggtat ttacacaggt tgttgatgaa aaacatcgta
    27601 ttatggccgc tgcatttcgg gagctacttt ctgaaataga agagctaagg acaattattg
    27661 actttggtga atacaaaccg ggggagaatg cctctcagga taaaatctac aacaaaatat
    27721 ctgttgttga gagttttctg aagcaagatt atcgactcgg ttttacttat gagcagacaa
    27781 tggagcttat tggtgaaaca attagataag gttttaaaaa tcaaagataa atatcaacgg
    27841 tctgttaaat taattgaagc acacatatta acactaaaaa aaaaactctt tgtatcgaga
    27901 tgtggaggca ttagataaaa ggattatata cttccagcaa ctggagaatg acttggaacc
    27961 tgtaggcgct cagagtgtta gtcagctttt caatacaaga cgtaagatcg ctatagtaaa
    28021 aaagcatatt atccaatatc agtctgaacg aattcttcta aagggaagaa ttgaagaaat
    28081 tcaaaaagat attgatgagg caaatgcttc taaaagaaaa ctgcttcata aagagagtaa
    28141 aatatgtaaa cggattggct tgattaaaag aaataacttt gccaaacagt tgatacttga
    28201 tgagttatca caagaggata tgaagtatgg cattagataa tataaaccta aattttagta
    28261 gtgacaaaca gatagaaaaa tgtgagaaac tatcttcaat agataatatt gatagtctcg
    28321 ttttgaagaa gaagaggaag gttgaaattc ctgagtactc tttaattgca tctaactatt
    28381 ttactattga taagcacttt gaacataagc atgataaagg agaaatttat agtggcatta
    28441 aaaatgcgtt cgaacttaga aacgaacgag cgacatattc tgatattcca gaatcaatgg
    28501 ccattaaaga aaatattttg ataccagatc aagatatcaa agcaagggaa aaaataaata
    28561 tcggcgatat gagggggatc ttttcatata ataagagtgg aaatgcagac aagaacttcg
    28621 aaagaagtca tacttcttct gtaaaccctg ataatctgct agaatctgat aatagaaatg
    28681 gtcaaattgg tttaaaaaat catagcttgt ctattgataa gaatattgct gacatcattt
    28741 ctttactaaa tggaagtgtt gctaaatcat ttgagctgcc tgtaatgaat aaaaatactg
    28801 cagacataac cccatccatg tcattgcaag aaaaatcaat agttgaaaat gataaaaatg
    28861 tttttcaaaa aaatagtgaa atgacttacc actttaaaca gtggggggct ggacattctg
    28921 ttagtatttc agtagagtct ggttcttttg ttctaaaacc gtcagatcaa tttgtaggaa
    28981 ataaacttga cttaattttg aaacaagatg ctgagggtaa ttacagattt gatagcagtc
    29041 aacataataa ggggaataaa aataatagta caggatataa tgaacagagt gaagaagaat
    29101 gctaagaatt aaacattttg acgctaacga aaaactacag attttatatg caaagcaact
    29161 ctgcgagcgt ttttcgattc agacattcaa aaataaattt acaggcagtg aaagtttagt
    29221 cactcttact tccgtgtgtg gggattgggt aattcgtatt gatacattat cttttttgaa
    29281 aaaaaaatac gaggtatttt caggattttc tacacaagaa tctttactgc atttatcaaa
    29341 atgtgtcttt atagagtcgt catctgtatt ttcgattcca gaactgtctg ataagattac
    29401 tttccggatc acgaatgaaa tccagtatgc aactactggg agtcatttat gctgtttttc
    29461 ctcttcttta ggtattattt attttgacaa gatgccggta ttacgtaatc aagtttctct
    29521 tgacttattg catcatcttt tagagttttg cttaggttca tctaatgtaa ggctggctac
    29581 tttaaaaaga attcgtactg gtgatataat catagttcag aaactttata atttattatt
    29641 gtgtaatcaa gttattattg gggattatat tgtgaatgat aataatgagg caaaaattaa
    29701 tctgtcagaa agtaatggtg agtcagatca cacagaagtt tctttggcat tattcaatta
    29761 tgatgatatc aatgtaaaag tggactttat ccttttagaa aaaaagatga caatcaatga
    29821 actaaaaatg tatgtagaaa acgaattatt caagtttccc gatgacatag ttaaacatgt
    29881 aaatattaaa gtaaatggtt ctttggttgg gcatggggaa cttgtttcta ttgaggatgg
    29941 ttatggtatc gagattagtt cttggatggt aaaggagtaa tctcatgctg agtgatatgt
    30001 ccctcatcgc tacactttct ttttttacgc tattaccatt tttagttgct gctggtactt
    30061 gttatataaa gttttctatt gtttttgtaa tggtacgaaa tgcacttggt ttgcagcagg
    30121 ttccatcaaa tatgactctc aatggtattg cattgattat ggctttgttc gtgatgaaac
    30181 ccattattga agctggttat gaaaattatc taaatggccc gcaaaaattt gatacaatat
    30241 cagatattgt ccgtttttct gattctggct tgattgaata caagcaatac ttgaaaaagc
    30301 atacagatct tgagttggca cgttttttcc aaaggtctga agaagaaaat gctgatttaa
    30361 agagtgcaga gaataatgat tattcattgt tttccctctt gcctgcatat gcgctaagtg
    30421 aaattaaaga tgcgttcaaa ataggttttt atttatattt gccatttatt gttgttgatc
    30481 ttgttatttc aagcatttta cttgcacttg gtatgatgat gatgagtcca atcacaatat
    30541 cagtaccaat aaaacttgta ttatttgttg cgcttgatgg gtgggggatc ctgtctaagg
    30601 ccttgattga acaatatatc aatgttcctg cttagatgat ggaggtaatg tgtctgatat
    30661 agtttatatg ggtaataagg ctctttattt aatccttatc ttttccttat ggccagtagg
    30721 tatagctacg gttattggat taactattgg tttattacag acagtgactc aacttcaaga
    30781 gcagacactt ccttttggta taaagcttat aggtgtctca atatctttgc tacttctttc
    30841 tggatggtat ggtgaggttt tattgtcttt ttgtcatgaa ataatgtttt taattaagag
    30901 tggggtttga tggacatttc aagctggttc gaaagtattc atgtgttttt aatactcctg
    30961 aacggcgttt tttttagatt ggctccatta tttttctttc ttccattttt aaataacggt
    31021 ataatttctc catctattag aatacctgtg atttttcttg ttgcatcagg attaattact
    31081 tctggtaagg tagacatagg ttcttctgtt tttgaacatg tttatttcct tatgttcaag
    31141 gaaataattg ttggcctcct tctctctttt tgcttgtctc ttcccttttg gatatttcat
    31201 gctgttggta gcattattga caaccagcgt ggggcaacgc ttagtagttc aattgatcct
    31261 gccaatggtg ttgatacgtc tgagttggca aaatttttca atcttttttc tgcagttgta
    31321 tttctataca gtggtggaat ggtctttatt ttagaatcca tacaattgtc ttataatata
    31381 tgcccgttat tttctcaatg ttctttccgt gtctcaaata tcttaacatt tctgacttta
    31441 ttggcaagtc aggctgttat tttagccagt cctgttatga tagtattgtt actatcagaa
    31501 gtattacttg gtgtattatc gagatttgct ccgcagatga atgctttttc cgtatcatta
    31561 actattaaaa gtttacttgc aatatttatt attttcatct gttcttctac tatttacttt
    31621 tctaaagttc aatttttcct cggtgaacat aaatttttca caaatctatt tgttagataa
    31681 aatattatgg caaataaaac agaaaagccg acacctaaaa aactaaagga tgccgcaaaa
    31741 aaaggacagt catttaaatt taaggattta acgactgttg ttattattct ggtagggaca
    31801 tttactataa tatcattctt ttccttaagt gatgtaatgc tcttatacag atatgtaata
    31861 attaatgact tcgaaattaa tgagggtaaa tacttttttg cagtggttat tgtctttttt
    31921 aagataattg gcttcccact ttttttctgt gttctttcgg ctgtgttgcc aacattggtt
    31981 caaacaaagt ttgttcttgc gactaaagct atcaagattg atttttcagt attaaaccct
    32041 gttaaagggt taaaaaaaat atttagtata aagacaataa aagaattttt caaaagcatt
    32101 ctgcttctta ttattctagc attaacaacc tatttctttt ggattaatga ccgaaaaata
    32161 attttttctc aggtgttttc tagtgttgat ggcttatatc ttatttgggg ggtgctgttt
    32221 aaggatataa tattattttt cttggcattt tctatttttg ttattattct tgactttgtg
    32281 attgagttca ttttatacat gaaagatatg atgatggata aacaggagat aaaaagagaa
    32341 tatatagagc aagagggaca ctttgagaca aagtcgagaa ggcgtgagtt gcatatcgag
    32401 attctttcag agcagactaa atctgatata cgtaattcaa aattagtggt aatgaacccg
    32461 actcatattg caattggtat ttattttaat ccagaaatag cgcctgcacc ttttatttct
    32521 ctcattgaaa ctaaccagtg tgccttggct gtcagaaaat atgcaaatga agttggtata
    32581 ccaactgtgc gtgatgtgaa attagctaga aaactatata aaacacatac aaaatatagt
    32641 tttgttgatt ttgaacactt ggatgaagtc ctacgtctta ttgtttggct tgagcaggtt
    32701 gaaaacactc attaataagt aaaggagatg tgttatatgg gagttaattt ctgtaataaa
    32761 ataggtattg atcagtctga atttgaaata gaatcttcca tcattaactc cattgctaat
    32821 gaggtattga acccaatatc tttccttagc aacaaggata taataaatgt tttactcaga
    32881 aagatttcat ctgaatgtga cctagttaga aaagacattt atcgttgtgc tctggagttg
    32941 gtcgttgaaa aaacacctga tgatttataa tcattcataa taactctttt gctgatataa
    33001 atatttttgt tgaagatatt tatgtagtca caggatcaat tgtagaaaat tgttatgttt
    33061 ttgttaggaa ctgcactaaa taatatatga taagacagca aaaacgactg accataatcc
    33121 ttcttttatt aggagttgat aagagagact attcatcctg taatgttaaa actctgttat
    33181 atagtattcg cgattatgca aagtctgtaa atgatcatga gatactaacc gagagtaatc
    33241 gtttattatc tcattgcatt tcagatagta acggagcttt ctttaaaagt agtaaatacg
    33301 ttccccttaa gtacttacgt aaaaggagaa tagcacgaaa gataccaaat gattagcact
    33361 ttgctagtaa gcttgctggt aaactatatt gaccgttggg tttgttttga gactgtaatt
    33421 taaattgtgt aattgcctgt ttttgatatg tttactccaa caacggagac aggtaaatta
    33481 tggacgaaaa gaagcttaaa gcccttgcgg ctgaactggc taaaggcctc aaaactgagg
    33541 ccgacctcaa tcagttttcc cgtatgctga cgaaa
//
LOCUS       DBKEKI010000034        33156 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.034, whole genome shotgun sequence.
ACCESSION   DBKEKI010000034 DBKEKI010000000
VERSION     DBKEKI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.034"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..500
                     /gene="mdfA"
                     /locus_tag="ACUQPX_001573"
     CDS             <1..500
                     /gene="mdfA"
                     /locus_tag="ACUQPX_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HHV9056320.1"
                     /translation="IITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLII
                     MGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVRLALFASDMSKG
                     TVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSLMVIFLKDKQMG
                     NSHEG"
     gene            complement(541..825)
                     /gene="ybjH"
                     /locus_tag="ACUQPX_001574"
     CDS             complement(541..825)
                     /gene="ybjH"
                     /locus_tag="ACUQPX_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HHV9056321.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(911..1726)
                     /gene="ybjI"
                     /locus_tag="ACUQPX_001575"
     CDS             complement(911..1726)
                     /gene="ybjI"
                     /locus_tag="ACUQPX_001575"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HHV9056322.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(1726..2934)
                     /locus_tag="ACUQPX_001576"
                     /pseudo
     CDS             complement(1726..2934)
                     /locus_tag="ACUQPX_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3018..3554
                     /gene="rcdA"
                     /locus_tag="ACUQPX_001577"
     CDS             3018..3554
                     /gene="rcdA"
                     /locus_tag="ACUQPX_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HHV9056323.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(3729..5414)
                     /locus_tag="ACUQPX_001578"
     CDS             complement(3729..5414)
                     /locus_tag="ACUQPX_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HHV9056324.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            5684..6061
                     /locus_tag="ACUQPX_001579"
     CDS             5684..6061
                     /locus_tag="ACUQPX_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HHV9056325.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(6091..6348)
                     /gene="grxA"
                     /locus_tag="ACUQPX_001580"
     CDS             complement(6091..6348)
                     /gene="grxA"
                     /locus_tag="ACUQPX_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HHV9056326.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            6508..6795
                     /locus_tag="ACUQPX_001581"
     CDS             6508..6795
                     /locus_tag="ACUQPX_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HHV9056327.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            6779..7501
                     /gene="nfsA"
                     /locus_tag="ACUQPX_001582"
     CDS             6779..7501
                     /gene="nfsA"
                     /locus_tag="ACUQPX_001582"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HHV9056328.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            7562..8464
                     /gene="rimK"
                     /locus_tag="ACUQPX_001583"
     CDS             7562..8464
                     /gene="rimK"
                     /locus_tag="ACUQPX_001583"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HHV9056329.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERWAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            8552..9028
                     /locus_tag="ACUQPX_001584"
     CDS             8552..9028
                     /locus_tag="ACUQPX_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HHV9056330.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            9379..10491
                     /gene="potF"
                     /locus_tag="ACUQPX_001585"
     CDS             9379..10491
                     /gene="potF"
                     /locus_tag="ACUQPX_001585"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HHV9056331.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            10586..11719
                     /gene="potG"
                     /locus_tag="ACUQPX_001586"
     CDS             10586..11719
                     /gene="potG"
                     /locus_tag="ACUQPX_001586"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HHV9056332.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            11729..12682
                     /gene="potH"
                     /locus_tag="ACUQPX_001587"
     CDS             11729..12682
                     /gene="potH"
                     /locus_tag="ACUQPX_001587"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HHV9056333.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            12679..13524
                     /gene="potI"
                     /locus_tag="ACUQPX_001588"
     CDS             12679..13524
                     /gene="potI"
                     /locus_tag="ACUQPX_001588"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HHV9056334.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            13584..14072
                     /locus_tag="ACUQPX_001589"
     CDS             13584..14072
                     /locus_tag="ACUQPX_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HHV9056335.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            14113..15240
                     /gene="rlmC"
                     /locus_tag="ACUQPX_001590"
     CDS             14113..15240
                     /gene="rlmC"
                     /locus_tag="ACUQPX_001590"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HHV9056336.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(15415..16146)
                     /gene="artJ"
                     /locus_tag="ACUQPX_001591"
     CDS             complement(15415..16146)
                     /gene="artJ"
                     /locus_tag="ACUQPX_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HHV9056337.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(16438..17106)
                     /gene="artM"
                     /locus_tag="ACUQPX_001592"
     CDS             complement(16438..17106)
                     /gene="artM"
                     /locus_tag="ACUQPX_001592"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HHV9056338.1"
                     /translation="MFEYLPELMKGLHTSLTLTFASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(17106..17822)
                     /gene="artQ"
                     /locus_tag="ACUQPX_001593"
     CDS             complement(17106..17822)
                     /gene="artQ"
                     /locus_tag="ACUQPX_001593"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HHV9056339.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(17829..18560)
                     /gene="artI"
                     /locus_tag="ACUQPX_001594"
     CDS             complement(17829..18560)
                     /gene="artI"
                     /locus_tag="ACUQPX_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HHV9056340.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(18578..19306)
                     /gene="artP"
                     /locus_tag="ACUQPX_001595"
     CDS             complement(18578..19306)
                     /gene="artP"
                     /locus_tag="ACUQPX_001595"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HHV9056341.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCSVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(19524..20039)
                     /locus_tag="ACUQPX_001596"
     CDS             complement(19524..20039)
                     /locus_tag="ACUQPX_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HHV9056342.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            20688..22457
                     /locus_tag="ACUQPX_001597"
     CDS             20688..22457
                     /locus_tag="ACUQPX_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HHV9056343.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            22668..22991
                     /locus_tag="ACUQPX_001598"
     CDS             22668..22991
                     /locus_tag="ACUQPX_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HHV9056344.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            22988..23818
                     /gene="amiD"
                     /locus_tag="ACUQPX_001599"
     CDS             22988..23818
                     /gene="amiD"
                     /locus_tag="ACUQPX_001599"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HHV9056345.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTSVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(23815..24828)
                     /locus_tag="ACUQPX_001600"
     CDS             complement(23815..24828)
                     /locus_tag="ACUQPX_001600"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HHV9056346.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKDISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(24927..26357)
                     /locus_tag="ACUQPX_001601"
     CDS             complement(24927..26357)
                     /locus_tag="ACUQPX_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HHV9056347.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(26368..27369)
                     /gene="ltaE"
                     /locus_tag="ACUQPX_001602"
     CDS             complement(26368..27369)
                     /gene="ltaE"
                     /locus_tag="ACUQPX_001602"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HHV9056348.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSRILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEIAAHWRAFLAR"
     gene            complement(27406..29124)
                     /gene="poxB"
                     /locus_tag="ACUQPX_001603"
     CDS             complement(27406..29124)
                     /gene="poxB"
                     /locus_tag="ACUQPX_001603"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HHV9056349.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTKFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(29257..30225)
                     /gene="hcr"
                     /locus_tag="ACUQPX_001604"
     CDS             complement(29257..30225)
                     /gene="hcr"
                     /locus_tag="ACUQPX_001604"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HHV9056350.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(30237..31889)
                     /gene="hcp"
                     /locus_tag="ACUQPX_001605"
                     /gene_synonym="priS"
     CDS             complement(30237..31889)
                     /gene="hcp"
                     /locus_tag="ACUQPX_001605"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HHV9056351.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDHIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(32033..32932)
                     /gene="lysO"
                     /locus_tag="ACUQPX_001606"
     CDS             complement(32033..32932)
                     /gene="lysO"
                     /locus_tag="ACUQPX_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HHV9056352.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8250 a         8210 c         8511 g         8185 t
ORIGIN      
        1 tcatcattac cggggagcag ttgagcagct atgaatatgg cttgctgcaa gtgcctattt
       61 tcggggcgtt aattgcgggt aacttgctgt tagcgcgtct gacctcgcgc cgcaccgtac
      121 gttcgctgat tattatgggc ggctggccga ttatgattgg tctgttggtc gctgctgcgg
      181 caacggttat ctcatcgcac gcgtatttat ggatgaccgc cgggttaagt atttatgctt
      241 tcggtattgg tctggcgaat gcgggactgg tgcgattagc cctgtttgcc agcgatatga
      301 gtaaaggtac ggtttctgcg gcgatgggaa tgctgcaaat gctgatcttt accgtcggta
      361 ttgaaatcag caaacatgcc tggctgaacg ggggcaacgg actgtttaat ctcttcaacc
      421 ttgtcaacgg aattttgtgg ctgtcgctga tggttatctt tttaaaagat aaacagatgg
      481 gaaattctca cgaagggtaa aaaaatgcct gattgatttg tgcgatcagg cattctcgaa
      541 ttaatggtga tggtcgtcaa tctggtgttc gataaccatc ccttcaccta cgctggcaag
      601 atggcgaaca taaggatgcg ggcggtaagc cggagctggc gcaggagcca catagacggt
      661 ttgcggcaca gtcgcgacgc tgaccgcttg tggaacgctg actgaatcag gaaccacaac
      721 cactttgtat ccactcggca catcgacggt gacactttgc gccatcgcca caccagtaaa
      781 gcccattaaa agcgcgccca acagtagaca atttttcata atcatttcgc ctgaaataat
      841 gcagcgggtt aatttccgcg aattatgcag ccttcattca ggcattgtgt tgccgggcgt
      901 gctgccgttt ttattggtca aatggcgctt cgtgtttaag aactttatcg atcacatcca
      961 gtacgccttc acggttattg gagcctgccc ggtattttgc cgctgcgacg accgcgctgc
     1021 cggcattttc cattgcaaaa ctaaagcctg cctgacgcag catctcaata tcgttaccgc
     1081 catcgccaaa gaccacaact tcgctgtcgt ctattcccca taatttctgc agttggcgaa
     1141 ggccattggc tttatgtacg ccggggataa tcagatcgat gctgccgttg ccggtgtgga
     1201 ctggcaccat aatatcgccg atggcctcat gtaatgcttt ttgtacttgt ggaatcagtt
     1261 catcggaaag attcaggcca aacttaaaga aaatatcttc taagttgtca aagttatcga
     1321 cgtattccag acggtgataa tacatttccg ccaccgtttt catggcatcg tcatattttt
     1381 tgagtgtata ggcgctattt tttccgcagg caataatttc cacttccggg cgcgtcagca
     1441 aatgttccac gacagtagca aacgcatcct tcgacagctc gccattaaaa acatctttgc
     1501 cttcactcac tacccagccg ccgttttcag ccacaaaggc aatttcattc gcaatttcag
     1561 ggaagaaaga gatcaactga taatattgat tcccgctggc gaccacaaag cgaatgcctt
     1621 gtgctttcat ttgctgatac tgagccataa accgctcacg gttataggtt ttttgatcgc
     1681 ttaagaaagt accatccatg tctaccgcaa ttaatttaat gctcatcaac tattctccat
     1741 cgccgtctgc gttttggtat cgggtttggc gacggctttc gcgacaatag ccgcgagaat
     1801 aaccagcgcc agtacaacca gcattgcact acgtaatcca taatgttcgc cgagatagcc
     1861 cagcagcggc ggcccgacga ggaaagccag ataaccggtc gttgctacca cactgacgcg
     1921 ggttggtgca tcggggccgg tatcgctggc ggcagaaatg gtcagcggga agcccagcga
     1981 ggcacccagt ccccaaagta caacagacac tcctgcgacc caggcgctat cgacaaaaat
     2041 aatcagccca atacccaacg cccccattag tgcactggcc cgaaccacgg caacgcgact
     2101 gtaacggtcg atgaaccaac cgccggtgaa gcgtccaacg gtcatcccca gggtaaaacc
     2161 ggcataaatc agtgagccgg aagtggggct aaaaccgtga ccatcaacca ttaataaggg
     2221 taactagtcg ttggcagaac cttcggcaaa ggccatcgcc agcactacaa caccaatcaa
     2281 cagcaactgg atatcgcgat aaaaaggaat gcctttttcg ccatgctggg tgccatcggc
     2341 agcatttttg cccgtaccgt cagggattgc ctgaatggcg atataaatag gcgcgatacc
     2401 taccagcgcc gccaataaaa tgtgcaccgt tgccggaacg ccaaaggccg tcagtgccat
     2461 cccgacacca gcgcctgcca gcgtgcccag gctataaaaa ccgtgcatca tcggcaaaac
     2521 cgttttattc atttctcgct caacggcggc accttcaacg tttatcgcca cttccgcaga
     2581 accaaaactt gccccaaaga cgccgagacc aacggcaaag agcaggggcg atgtcagcca
     2641 gagtgccaga cttaatatca tcatcccgat caatgcgcag gacatcgtga ccaggatgac
     2701 attacgtgtc ccaaagcgtt tcactaacca cgccgagcag agaataccgc tcatcgaacc
     2761 gatcgacaga ccaaagagaa caccgcccat ttcagcgatc gagacagaga gaatatcgcg
     2821 gatagcaggc gtacgggttg cccaggacgc cattaacagg cctggcaaaa agaagaacat
     2881 aaacagcgcc caggttcggc gtttcaatgc attacgtgaa gaatttacgg tcatagatca
     2941 cgtcaaaata agaagaggga agacaacatt agcaaggttg tgtacatttg tacacaattg
     3001 cagacagagg aaatgacatg cgtcgcgcta acgatccgca acggcgagaa aaaattatcc
     3061 aggccacgct ggaggcggtg aaactttacg gaatacatgc tgttacgcac cgcaaaattg
     3121 ctacccttgc cggggtaccg ttgggatcga tgacctacta tttttcagga attgatgagt
     3181 tgttgctgga ggcgttcagc agttttactg agattatgtc ccggcaatat caggcatttt
     3241 ttagcgatgt tagtgatgct caaggcgcat gccaggctat caccgatatg atctacagct
     3301 cacaggttgc aacgccggat aatatggagc tgatgtacca gctctacgcg ctggctagcc
     3361 gaaaaccgct attaaaaacg gtaatgcaaa actggatgca gcgcagtcag caaacgctcg
     3421 aacaatggtt tgaacccgga accgcccgcg cgcttgatgc gtttattgag gggatgacgc
     3481 tgcattttgt caccgaccgt aagccgctat cgcgcgagga gattttgagg atggttgaga
     3541 gggttgcagg gtagtagata aattttagat aacaaaaaac ccatcaacct tgaaccgaaa
     3601 tggcggggtt gatgggctcc acaaaatggg gacatcaaag aaaagcagtg gcactaatta
     3661 agactgatgc cctgcggaaa agttctgcgg ttgtgcaaaa aaatttcatt ttcagggcaa
     3721 cttcagtttt atcctaatcc tggccatacc atgacgatga ttgtccctgc cagcgtcagc
     3781 aggacgttgg cgattgcgta ggtgcccgca tagcccagcg cagggatgtt actgcgagct
     3841 gtatcactga tgatctccat tgccggcgcg caggtgcgtg cgcccatcat tgcgccgaac
     3901 aacagtgcgc ggttcattcg caatacataa gcaccgaaca agaaacagat aaccacgggc
     3961 acaagactga caattaatcc ggcaatcaac atctgaccgc caatcgcgcc caggccgtta
     4021 ttaataccgc taccggcgct cagaccaacg cctgccataa acaccatcaa gccgaactct
     4081 ttcaccatgc ttaatgcacc ttgcggaatg taaccgaagg tcgggtggtt agcacgcata
     4141 aagcccagca taattccggc gaataacaac ccggcagcgt tccccatgcc gaaactgaat
     4201 gtgctgaact ggaaggtgat catcccgatc atcagcccaa taacaaagaa ggcgcaaaat
     4261 gccagcaggt cagtgacctg gctgtgaatc gagataaagc cgatgcgatc ggcgatggtt
     4321 tttacgcgac gggcatcacc gctgacttgt aaaacgtcac ctttgttaag tacgacgttg
     4381 tcatctatcg gcatctcaat ctggctacga atgacgcggt taaggaagca accgtgatcg
     4441 gtcaacttca gttgtgcgag acgtttacct acagcgttat ggtttttaac gaccacttct
     4501 tcagtgacga tacgcatgtc gagaaggtca cgatcgaaaa cttctttacc gttacggaag
     4561 ctgggatcga gtcgggcatg ggcgtcggga tagcctacca acgctatttc atcgcccatt
     4621 tgtagcacgg catcaccgtc tggatttgcc agaatcccgt tacgtcgaat acgttcaatg
     4681 tagcagccgg tttgtcgata aatacccagt tcacgcagat ttttgccgtc ggtccaggcc
     4741 accagctccg ggccgacgcg ataggcgcgg atcaccggta aataaacctt acggttggca
     4801 tcagtgtcca ggccacgttc gcgggcgatt tgctgggcgc tggtctgtaa gtcctgatgc
     4861 tgcaatttcg gcaagtaacg cgcaccaaca atcaaactca ccagaccgat taaataggtt
     4921 aaggcatacc cgaggctcag attatccagt gccagtgaga gctgcctgct ttccatgccg
     4981 gaatgacgca gtgtatcgcc agcaccgacc agaaccggtg tcgacgtcat agagcctgct
     5041 aacataccgg ccgtcaggcc aatatcccag ccaaacagct tacctaaccc taaggcgatc
     5101 accagcgcac tgccaaccat caccagtgct aacattaggt aatttttccc atcgcgaaaa
     5161 aaaatggaaa aaaagttcgg tccggcttcg accccgacgc agaaaataaa cagcataaag
     5221 ccaagattaa gcgcatcggt gttaatgctg aaatgttgtt ggcctaataa cagcgatacg
     5281 actaaaacgc caatggaatt acccagttgg atcgaaccaa gtcgtaactt tccgagacat
     5341 agcccaagcg cgaggaccac aaataataac agaatgtaat tcccatttaa caattcggcg
     5401 acgtttatat tcacggaggc taacttcttg tttactagta agctgttgaa agaaatggta
     5461 atttacgata atgtttttta ccagaattca gggcgcagat tcattcagcg cacctaaacg
     5521 atagtaaagt aacaatatat tttactagtg taatcacatt aggtatcaac ggctatatga
     5581 attgcgttgg cctatattag catggaatgc gaagcggctt tatcttactg aacgccacac
     5641 tggcgaaaaa tgtgttcgat agacgcagtg tcaggaggaa cgagtgaaac ataaacaacg
     5701 ttgggcgggg gcaatctgct gttttgtcct cttcattgtg gtgtgccttt ttctggcgac
     5761 gcacatgaaa ggcgcttttc gggctgccgg gcatcctgaa atcggcttgc tgtttttcat
     5821 tcttcctgga gcagttgcca gtttcttttc acagcgtaga gaagtcctga aacctctatt
     5881 tggcgcaatg ctggcggcac cctgttcaat gctcattatg cggctgtttt tttcaccgac
     5941 gcgctcattc tggcaagagc tggcatggtt actaagcgcg gtgttctggt gtgcgctggg
     6001 ggcactgtgt ttcttattta tcagtagttt gtttaaacca cagcacagaa aaaatcagta
     6061 aagccctcaa cgcgagggct tgtcagacga tcaggcgtcc agattttctt tcacccatgc
     6121 agcaaaatcg gtatagccgc cgatatgttg ctgatcgaca aaaatctgcg gcacggtttc
     6181 tacgggttta cctgcctttt gttgtagatc ttctttagtg atcccttccg cacgaatatc
     6241 tacatactga tactgaaaat catcgcgttc attgctcaat ttctcagcca gatcttttgc
     6301 acgcacacag taagggcaac ccgaacgacc aaaaataacg gtttgcatta tttctctcct
     6361 catagattta tgcctgtaat gatcacgcta aaatgtattc gctgaaagta ggtttaacct
     6421 gttgcattaa ttgctaaaag ctataactgt taaacacaat acagtgaaaa gttttagact
     6481 gaaggctcac tttgcagagg gaagcgtatg cgcgcgatcg gtaaattgcc taaaggcgtg
     6541 ttgatactgg aatttatcgg aatgatgcta ctggcggtgg cgctgctgtc ggtaagcgac
     6601 tccctgtcgc tgcctgagcc attttctcgg ccagaagtgc agattctgat gatttttctc
     6661 ggtgttttgc tcatgcttcc cgctgcggtg gtggttattc ttcaggtggc aaaacgtctt
     6721 gccccacagc tgatgaaccg tccaccgcaa tattcacgtt cagaaagaga aaaagataat
     6781 gacgccaacc attgaactta tttgtggcca tcgctccatt cgccatttca ctgatgaacc
     6841 catttccgaa gcgcagtgtg aggcgattat taacagcgcc cgtgcgacgt ccagttccag
     6901 ttttttgcag tgtagtagca ttattcgcat taccgacaaa gcattacgtg aagaactggt
     6961 gacgctgacc ggcgggcaaa aacacgtagc gcaagcggcg gagttctggg tgttctgtgc
     7021 cgactttaac cgccatttac agatctgtcc ggatgctcag ctcggcctgg cggaacaact
     7081 gttgctcggt gtcgttgata cggcaatgat ggcgcagaat gcattaaccg cagcggaatc
     7141 gctgggattg ggcggggtat atatcggcgg cctgcgcaat aatattgaag cggtgacgaa
     7201 actgctgaaa ttaccgcagc atgttctgcc gctgtttggg ctgtgccttg gctggcctgc
     7261 ggataatccg gatctcaagc cgcgtttacc ggcctccatt ttggtgcatg aaaacagcta
     7321 tcaaccgctg gataaagacg cactggcgca gtatgacgag caactggcgg aatattacct
     7381 cacccgtggc agcaataatc gccgggatac ctggagcgat catatccgcc gaacaatcat
     7441 taaagaaagc cgcccattta ttctggatta tttgcacaaa cagggttggg cgacgcgcta
     7501 aaaccgccac gtcgatgtat gatacgcggg cttttgacca ggtctgacag agaggtgcag
     7561 ggtgaaaatt gccatattgt cccgggatgg aacgctctat tcgtgtaagc ggctgcgtga
     7621 agccgctata cagcgcggtc acctggttga aattcttgat ccgctttctt gctacatgaa
     7681 cataaatcct gcggcgtctt ctattcacta caaaggccgc aagttacccc attttgacgc
     7741 agtgatcccg cgtattggca ccgccattac cttttatggg acggcggcac tgcgccagtt
     7801 cgagatgctg gggagctatc cgctcaatga gtcggtcgcc attgcccggg cgcgtgacaa
     7861 attgcgttcc atgcaactgc tggcgcgtca gggcatcgac ctgcctgtca cgggcattgc
     7921 gcattcgccg gatgatacca gcgatttaat cgacatggtc ggtggtgcgc cgctggtggt
     7981 caagttggtt gaaggcacgc agggaattgg cgtcgtgctg gcggagactc gtcaggcggc
     8041 ggaaagcgtg attgacgctt tccgcggtct gaacgcgcat attctggtgc aggaatatat
     8101 caaagaggcg caagggtgcg atatccgctg tctggttgtt ggtgatgaag tggtcgctgc
     8161 gattgaacgg tgggcgaaag agggctattt tcgttccaat ttgcatcgtg gcggcgcggc
     8221 aagcgtcgcc agtatcacac cacaggagcg tgaaatcgcg ataaaagccg cgcgaacgat
     8281 ggcgctggac gttgctggtg tggatattct gcgtgctaat cgcgggccgt tggtgatgga
     8341 agtgaatgcg tcgccggggc tggaaggaat agaaaaaacc accggtatcg atatcgcggg
     8401 taaaatgatc cgctggatcg aacgccacgc tacgacagaa tattgcctga aaacgggtgg
     8461 ttagtcgcaa tcacattact gatcatggtt ttgcctgcgc tttttgcgta agctgtgccg
     8521 gtctttttat cgaaagaggt tgtacaaaat tatgacatcg ctggtcgttc ctggtctgga
     8581 tacgctgcgt caatggctcg atgacctggg gatgagtttt tttgaatgtg ataactgtca
     8641 ggctctgcat ctgccccata tgcagaattt cgacggtgtc tttgatgcca aaatcgatct
     8701 gatcgataac acgatcctgt tttctgccat ggcggaagtc cgaccttcag ccgtattgcc
     8761 gctggcggcg gatttatctg ccatcaatgc cagttcgctg accgtgaaag catttcttga
     8821 tatgcaggat gataatctgc caaagctggt ggtttgccag tctttatccg ttatgcaggg
     8881 cgtaacctat gagcagtttg catggttcgt gcgtcagagc gaagagcaga tttcgatggt
     8941 cattcttgaa gctaatgccc atcaactgct gttaccgact gacgatgaag ggcaaaacaa
     9001 cgttaccgaa aactattttc tccactgata attcctttcg agcacgcagt cgctgccgca
     9061 gtgactgcgt gctgcaaaat tatctgctgt tttaaccttt tcttaaagat tatttcactt
     9121 ctcttgtgtc gatttggctt tatcacatag agcaaatacg cataaaaatt tgttaaatac
     9181 cgttttttaa tccgagctat agtctcaaac cctggctaaa gttattcttg cgatactttt
     9241 atatagcgag cagtgctggc cgggagagag ttctcttttc ttacaccgcg ccgataaaaa
     9301 aatatgcacg tttattgcat atctttcagt gtgacaactt ttgttcgttt gttaacgaac
     9361 tttcagaagg aaagagatgt gaccgcctta aataaaaaat ggctatcggg tctggttgcg
     9421 ggtgctctga tggccgtctc tgtcggcacg ctcgcggctg aacaaaaaac actccacatt
     9481 tataactggt ctgattatat cgcccctgac acggtggcca attttgaaaa agaaaccggt
     9541 attaaagtcg tctacgatgt tttcgactct aacgaagtac tggaaggcaa attaatggcg
     9601 ggtagcaccg gctttgactt ggtggttcca tctgccagct ttcttgaacg ccagttgact
     9661 gcgggagttt tccagccgct ggacaaaagc aaattgccgg agtggaagaa tctcgacccg
     9721 gagctgctga agctggtcgc caaacacgat cccgacaata aatttgctat gccctatatg
     9781 tgggcgacga ccgggattgg ctataacgtt gataaagtta aagctgtgct gggcgaaaac
     9841 gcgcccgtcg atagctggga cttgatcctc aaacctgaaa atctggaaaa actgaaaagc
     9901 tgcggtgtct ctttcctgga tgcgccagaa gaagtttttg ctaccgtgtt gaattatctc
     9961 ggcaaagatc ccaacagcac taaagcggat gattacaccg gaccggcaac cgatctgctg
    10021 ttaaagctgc gcccgaacat tcgttatttc cattcatctc aatacattaa cgacctggca
    10081 aacggcgata tttgcgtcgc tatcggctgg gcaggtgatg tctggcaggc gtcaaaccgc
    10141 gcgaaggaag cgaagaatgg agtgaatgtc tcgttctcga ttccaaaaga aggggcgatg
    10201 gcgttctttg atgtattcgc catgcctgcg gatgccaaaa acaaagacga agcctatcag
    10261 ttcctgaatt acctgctgcg cccggatgta gtagcgcata tttccgacca tgtgttctat
    10321 gccaacgcca ataaagcagc cacgccgctg gtgagtgcgg aagtccgtga gaacccgggt
    10381 atttatccgc ctgcggatgt tcgtgcgaag ctgttcactc tgaaagtgca ggacccgaaa
    10441 atcgaccgtg tgcgcacccg cgcgtggacc aaagtgaaga gcggaaaata atccgcagcc
    10501 ttagatgccg ggcgggcgca ccacacccgc cggcaattcg caccatcatg gtgcgcttgc
    10561 acacattcaa tgccggagag cagccgtgaa tgacgctatc cctcgcccgc aggcgaaaac
    10621 ccgtaaggcg ctgacgccgc tattagaaat ccgcaacctc accaaatcct acgatggtca
    10681 acatgcggtg gatgatgtca gcctgaccat ctacaaaggt gaaatcttcg cgctgctggg
    10741 cgcatccggc tgtggcaagt ccacgctgct gcgtatgctg gcaggtttcg aacaaccttc
    10801 tgccggacag ataatgcttg atggcgtcga tttgtcacag gttccgcctt acctgcgccc
    10861 catcaatatg atgtttcagt cttacgcgct gtttccgcat atgaccgtgg aacagaacat
    10921 cgcttttggc ctgaaacagg acaaactacc gaaagcggaa atttccagtc gggtcaatga
    10981 gatgctcggc ctggtgcaca tgcaggagtt cgccaaacgt aaaccgcatc agctttctgg
    11041 tggtcagcga cagcgtgtgg ccctggcccg aagccttgcc aagcgcccga aactattact
    11101 gctcgacgag ccgatgggcg cgctggataa aaagctgcgt gaccggatgc agcttgaagt
    11161 ggtggatatt ctggaacgcg tcggtgtgac ttgtgtgatg gtcacccacg atcaggaaga
    11221 ggcgatgacc atggcggggc gcatcgccat tatgaatcgt gggaaatttg tccagattgg
    11281 cgaaccggaa gagatctacg agcatccgac tacccgctat agcgccgaat ttattggctc
    11341 ggtaaacgtc tttgaaggcg tactcaaaga gcgtcaggaa gatggcctgg tgcttgattc
    11401 gccggggctg gtgcatccac tgaaagtcga cgcggatgcc tcggtggtcg ataacgtgcc
    11461 ggtacatgtg gcgctgcgcc cggaaaaaat catgctttgc gaagagccgc ccgccaatgg
    11521 ttgtaacttc gcggtggggg aggtgataca cattgcctat ctcggcgatc tttcggtgta
    11581 tcacgttcgt ctgaaaagtg ggcagatgat tagcgcccag ctacaaaacg cccatcgtca
    11641 tcgtaaaggg ttaccgacct ggggcgacga agtgcgtttg tgctgggaag tggacagctg
    11701 tgtggtgctg acggtttaag gagcaaagat gaatacactt gaacctgctg cccagtcgaa
    11761 accgccgggc ggatttaagc tgtggttgtc gcagctgcaa atgaagcatg ggcgcaaact
    11821 ggtcattgcg ttgccatata tctggttgat cttgctgttt ctgctgccat ttctgattgt
    11881 ctttaaaata agcctggcgg agatggcgcg cgctattcca ccttataccg aactgatgga
    11941 gtgggctgac gggcaactgt ccatcactct taatctcggt aattttctgc aactgaccga
    12001 cgatccgctc tatttcgatg cttatctcca gtcgctacag gtggctgcga tttcgacatt
    12061 ttgctgttta ctgatcggct atccgctggc gtgggcggtg gcgcacagta agccttcgac
    12121 ccgtaatatt ttattactac tggtgatcct gccgtcgtgg acctcgtttc tgatccgcgt
    12181 ttatgcctgg atgggaatat taaaaaacaa cggtgtgctg aataattttc tgctgtggct
    12241 gggggttatc gatcaaccgc tgaccattct tcataccaat ctggcagttt atattggcat
    12301 tgtttacgcc tacgtgccgt ttatggtgct gccgatttat accgcgttga ttcgtattga
    12361 ttattcgctg gtggaagcag cgctggatct cggtgcacga ccgctgaaaa cgttctttac
    12421 tgtgatcgtg ccgctgacta aaggtgggat tattgccgga tcgatgctgg tgtttatccc
    12481 ggctgtgggc gagtttgtga tcccggaact gctcggtggc ccggacagca tcatgatcgg
    12541 gcgcgtgcta tggcaagagt ttttcaataa ccgcgactgg ccggtggcct cggcggtagc
    12601 gatcatcatg ttgctgctgc taattgtgcc gataatgtgg ttccacaaac accagcaaaa
    12661 aagcgtggga gaacacggat gaataattta ccggtagttc gttcgccctg gcggattgtg
    12721 attttgctgc tgggctttac ctttctctac gcgccaatgc tgatgctggt tatctattcg
    12781 tttaacagct cgaagctggt gacggtgtgg gccggctggt caacgcgctg gtatggtgag
    12841 ttattgcgcg atgacgcgat gatgagtgcg gttggtttaa gcctgacaat tgctgcctgt
    12901 gcggcaacgg cggcggcgat cctcgggact attgcggcgg tggtgctggt gcgctttggc
    12961 aggtttcgcg gatcaaatgg ctttgccttt atgatcaccg cgccgctggt aatgccagat
    13021 gtcatcacgg gcttgtcgct gttgttatta ttcgtcgcgc ttgctcatgc cattggctgg
    13081 cctgcggacc gcggtatgct taccatctgg ctggcgcatg tcactttctg tacggcttat
    13141 gtggcggtcg ttatttcgtc gcgtctgcgg gaactggatc gctcgataga agaagccgcg
    13201 atggatctcg gtgcgacgcc gctgaaagtg tttttcgtca ttacgctacc gatgatcatg
    13261 cccgcgatca tttctggctg gttactggct tttactttgt cgcttgatga tctggtgatc
    13321 gccagctttg tttccgggcc gggagccacc acgttaccga tgctggtctt ttccagcgtg
    13381 cggatggggg tgaatccgga aatcaacgcc ttggcaacat tgatcctcgg cgcggtcgga
    13441 attgtcggat ttatcgcctg gtatctgatg gcgcgcgcag aaaaacagcg gatacgcgac
    13501 atccaacgtg caagacgtgg ctgaagacac taaaatttgc caacctggct acataatgcc
    13561 gcgcatgtcg cggcattgtt ttcatggaag acgaaacgtt gggatttttt aagaaaacat
    13621 cttcatctca tgctcgcctg aatgtgcctg cgctggtgca ggtggcggcg ctcgccatta
    13681 ttatgatccg tggcctcgac gtgctgatga ttttcaatac gctgggcgtg cgcggtattg
    13741 gcgagttcat tcatcgcagc gtacaaacct ggagtttaac gctggtcttt ttaagcagtc
    13801 tggtgctggt tttcattgag atctggtgtg cgttttcact ggtgaaaggg cgtcgctggg
    13861 cgcgctggct atatctgctg acacaaatca ccgccgcaag ttacttgtgg gcggcttcgc
    13921 tggggtacgg ttatccggag ctgttcagca ttcccggtga atcaaaacgt gaaatcttcc
    13981 atagcctgat gctgcagaag ctgccggata tgctcatcct gatgctgctg ttcgttccct
    14041 cgaccagtcg gcggttcttc cagttgcaat aatgtgtata atcgtcgccc ctgatgatgt
    14101 gaaggtcaat gtatgcagtg cgcactttac gacgcgggtc gctgtcgttc ctgtcagtgg
    14161 ataacgcagc cgattccaga gcaactctcc gctaaaaccg ccgatcttaa aaatctgctc
    14221 gccgattttc cggttgagga atggtgcgcg ccggtgtcag gcccggaaca agggtttcgt
    14281 aataaagcca aaatggtggt gagtggtagc gttgaaaaac cactgctcgg tatgctgcat
    14341 cgcgatggca cgccggaaga cctttgtgac tgcccgcttt atccagcctc atttgcgccc
    14401 gtttttgcgg cgctaaaacc gtttatcgcc cgagcggggt taacgcccta caacgtggcg
    14461 cgtaagcgtg gcgaactgaa atacattctg ctgactgaaa gccagagtga tggcggcatg
    14521 atgctgcgct ttgtactgcg ttctgatacc aagctggcgc aactgcgtaa ggcgctgccg
    14581 tggttacacg aacaactacc gcagctgaaa gttattaccg tcaatattca gccggtacat
    14641 atggcgatta tggaagggga gacggagatc tacctgaccg aacaacaggc actggcggag
    14701 cgttttaatg acgtaccgct gtggatccgt ccgcaaagtt tcttccagac taatccggcg
    14761 gtcgccagcc agttgtacgc caccgcgcgc gactgggtac gacagctgcc ggttaaacat
    14821 atgtgggatc tgttctgcgg tgtggggggc tttggtttac actgcgcgac gcctgacatg
    14881 cagttaaccg ggatcgaaat tgcgccagag gccattgcct gtgcaaagca gtcagccgct
    14941 gaactgggct taactcgttt acaatttcag gcgctggact ccactcagtt tgccactgct
    15001 cagggggagg tgccggagct ggtgctggtt aacccgccgc gccgcggcat tggtaaaccg
    15061 ctgtgtgatt atctttcaac gatggcaccg cgttttatta tctactccag ctgtaacgcc
    15121 caaaccatgg cgaaagatat ccgcgaactg ccaggttacc gcatcgaacg ggtacagctt
    15181 tttgatatgt tcccgcacac cgcgcactat gaagtgctga cgctgctggt gaagcaataa
    15241 aaaagccaca ggtgcggctt cagattgctg atcaggtgcg ctttgttcat gccggatgcg
    15301 gcgtgaacgc cttatccggc ctacaaaatc atgctaaatc aatacattgc agagttgatg
    15361 tgggccggat aagcgcagcg catcaggcaa tcttactttt gtcatcagtc tcaattactg
    15421 cggaaaccac tggtcattga ttttctgata tgtaccgtca gctttaattg ctgccagcgc
    15481 gttattcagt ttttccagca gagctttgtt atccggacgt acagcgatac ccaggccggt
    15541 gccgaagtat tgcggatcgg tcactttttc agtcgcaacg cccagttgcg gattggtttt
    15601 cagccattcg tttaccaccg cggtgtcacc aaatacccca tcaatacgac catttttcag
    15661 atcgataaag gcattctgat aactgtcata ggagacagtt ttcacttccg ggtgctgatc
    15721 ctgaatatat ttctggtgcg tcgtaccgtt ttccatccca atgcgtttgc ctttcaggtc
    15781 ggcaaacgtt ttgtaggtat cttttttggc aatcacgacg gctgagtttt catagtaggg
    15841 tgtggtaaac gaaacctgct tgctacgctc cggggtgata tccataccgg agattacggc
    15901 gtcatatttt ctgaatttca gtgacgggat caggctgtcg aacgcgtgat tagtaaaagt
    15961 acattctgcc tgcatttgtt tgcacaaggc ttttgccaga tcgatatcaa agccgacaat
    16021 ctcattatta gcacctatag attcaaaggg tggataggtg gctgaaacgc caaaattgat
    16081 tttctctgcg gcagaagcac cgaaagtaaa ggaagcaagt aaagcggcaa gaactaactt
    16141 tttcatgatg gaactcccgt ctgtcaatct tatgattttt ggccgtgtct gcggcatggg
    16201 ataacaatgc caccaagtga atttatatgc aataaacatg attaaataat ttaaatgaaa
    16261 taaaaaaaga cggataactt agtgagttgt ccgtcttcat tataagaatt tatgcactat
    16321 gtaggccgga taaggcgtcc acgccgcatc cggcacaggc accgtgctga tgtctgatgc
    16381 gacgctggcg cgtcttatca gacctacaaa cccccccggc gaatgtacgc agctacatta
    16441 attccgccgt tcgaatgcca gtgctttgcg ctcgatcaga cgcatcatca gcgtcagcag
    16501 gccgttgacg accaggtaaa taatccctgc cgcaccgaac accattacat cgtaggtgcg
    16561 tccgtacaac aactggctgt atcccatcac ttccatcagc gtaatggtgt aagccagaga
    16621 ggtacttttg aacaccagca ccacttcgtt ggaataagaa gagagcgagc gtttaaaggc
    16681 atacggcagc aggatcgcca gcgtatcttt tttgctcatt cccagggcgc tacaggactg
    16741 ccactgacct tctgggatcg cacgaattgc accgtaaaac agctgcgtgg tatacgccgc
    16801 actgttcagc gataacgcaa tcagcgcaca taaccacggt tctgacaaca aatgccacag
    16861 tgccggatac tcctgcaacg tcggaaactg gcccggcccg taataaatca ggaagatctg
    16921 caccagcagc ggcgtaccgg taaacagcgt gatataaccc cgcaccagcc acaccagcac
    16981 cggcgttttc agcgtcagga tgatggtaaa aatcaatgcc agaatcagtg ccacaatcag
    17041 cgaggcaaag gttagcgtca ggctggtgtg cagccctttc atcagctcgg gtaaatactc
    17101 aaacattagc tgggcctccg ctcaaaacgt gtcgcgcgca ggtcaatgcg tttgagaatg
    17161 tactgactga gcagggtgat caccaggtaa atcgccgccg ccacaatgta ccaggtaaat
    17221 ggttcctggg tacgagtagc aatgcttttg gtttgtaaca ttaaatcatt cacactaatc
    17281 aaactgacca gcgcggtatc tttcagcagc accagccact ggttaccgag gccaggcagc
    17341 gcatgacgcc acatctgcgg catcaccaga cggaaaaaga tagccgattt cgacagcccc
    17401 agcgcctgac cagattccca ctggcccacc ggtacagctt tcaacgcgcc gcgcagcgtt
    17461 tgcgaggcat aggcggcata cagcagtgac agagcgatga caccacaaag gaacggactc
    17521 acgtcgaagt tctcaatgtc catctgcacc gggatctgca cgaacccaag attgatagtg
    17581 aagccatccg aaagcgtcag cagtaactgc gaggagccaa aatagataaa cagcactacc
    17641 agaatttctg gcaggccacg cagaatggtt accagcgctg aacctgccca cgcgacagga
    17701 cgccatttcg ctgactccca taccgcaaag aacatcgcca gcgccagccc gacaatcaat
    17761 gcacaaacgg caaggccgac ggtcatcccg gcggcgcttg ctaaaggaaa aaattcattc
    17821 atcaggaatt acttctggaa ccatttgttg tagatggttt cgtaagtgcc atctttcttc
    17881 actttttcca gcgcagtgtt gagtttctgc tgcagttcag tgttgccctg acgtaccgcg
    17941 atgccgaggc cagtaccgaa ataatcttta tcggtcactt tatcgcccac tgccgccagt
    18001 tttgggttat ctttcagcca ctcagtgacc actgcggtgt caccaaatac gctgtcgata
    18061 cgcccgtttt gcagatccag ttttgcgttc tggtagctgt catacggaac ggtggtgatt
    18121 tccgggtgct tatccataat gaacttctgg tgtgtcgtcc cgttctgaac gccgactttt
    18181 ttgcctttca gctgatcaac actggtgtat ttgccttgct gacccacaaa cagggcagag
    18241 ttgtcatagt acggggtggt aaacagtacc tgcttttcac gctccggagt gatatccatg
    18301 cccgccatca ctgcttctac gcgacggaat ttcaggcttg ggatcaggct gtcaaacgcc
    18361 tggttagaga aagtacaggt tgcatcaatc tctttacaca gtgcctgtgc caggtcgacg
    18421 tcaaaaccaa cgatctggtt gtttgcatca atcgattcaa acggaggata ggaggcttcg
    18481 gtagcaaaac gaatggtttc ggcagctgtg gcggaaagac taaaacctgc aattaacgcg
    18541 gcaatcagaa cttttttcat tgttgttatc ccgaatctta gtgagagaga tagtttttaa
    18601 atgcttcggt ttgcggctcg gtaaagcagc tcgcgtcgcc ttgttctacg atatgaccat
    18661 tttccatata caccactcgg ctggcggttt tacgcgccac ttcaacttcg tgggtgacga
    18721 tcacctgggt aatattcgtt tctgccagct cacgaatgat gctgacgatt tgtgccgtaa
    18781 tttccgggtc cagtgcggcg gtcggttcat cgaacagcag tacctgcggt tccatcatca
    18841 acgcacgggc aatagcaaca cgctgctgct gaccaccaga aagatgcagc gggtaacgat
    18901 cgctataagg tttgagacgc agacgttcca gcagtttttc tgcacgggcc agcgcctgat
    18961 ctttactcaa ccccagtaca ctgcagggcg cttcaatcag gttttgctgc acggtcagat
    19021 gcggccacag gttgtattgc tgaaacacca tgccaacgtt acgacgcaaa tcgcgaatcg
    19081 ctttgtcaga gggtgttttg gtgaaatcga aatggttgcc tgcaatgttg agcgtaccgg
    19141 agcgcggcat ctcaagcaga ttgagtacac gcagcagcga gcttttaccc gcgccgctgg
    19201 ggccaagtaa caccagcgtt tcgccctgtg ggcaatccag cgtgatatcg aacagcgcct
    19261 gatgcgcgcc gtagaagcaa ttaatgccgt ttaattgaat actcattgac actcgtatac
    19321 tggcagtctg atagctattg aggtcgaaga tagtaccttt gacagaataa ttatgcaata
    19381 tttatgcttt aaaagttaaa agcaaagcgc attattcaat aaacatagca caaaataacg
    19441 ggggcggtgg tcggtgagca taaatgtcgg cattcctcac gaaatgccgg ataatttacg
    19501 gggtttattg gttgatcaag gcgttagcga ttctcgatgg actgacggag cgtacccgcc
    19561 gtggcatgaa cgctaccgcc taagtagcgc acatcgtcga tgacccaaca ctggccttcc
    19621 tgaatcatta acacttcatc ctgccagccc tggtcaccct gtttgagatc cacccgcaat
    19681 ggaatgttac gggcatcacg attagggata gtcgatgcac tggcaacgtg ggcgctgtct
    19741 ggcaaggtgg ttcgactgga gaacggatcg ctgcttaaca attcacgatg actattatcg
    19801 cggctggcat cgctaagttg ggttgccagt ttgtcactca aataggggcg cagggcggta
    19861 atgtcattgc tgcgatgttg aatgcggtag tcgtagaatt gttgagccac actgtcaggc
    19921 cctccctgga cacaggaacc ggtgcggggg ccgttatctt tataagctgg cgtgacggtg
    19981 gtacaggcac tcagcagcag tgcacatgga ataagtagag atagtgaacg gtagcgcata
    20041 atcatttcct tattagcttg ctgtctgcgt cacactacga ccaaacgcta tcagattatt
    20101 taatacattg tttttaatac aattctgaaa caatctgaac acctgattgg gtttgagtcc
    20161 attgctggct acccactcaa ggttgctcgt agctgactta cttaaattac ccaaacaata
    20221 gacagtgtaa accatttgtg aggttgagcg tgttatatgg ctgaaatgat catttaaggt
    20281 aaatatgata gttctgaaat ggtggtataa atcctcctgt agaggttaag agcatctgca
    20341 agaagccagt ttcacattta taacgcgtga taaatgaatg cggttattgt ctaatcatta
    20401 caaaattttt cacatgcgat aagagatcct gcgattaaga tgattgccaa aattacgttg
    20461 tatttttgaa aatgagcgaa atttgatagt cccagctttg actctcgata atattattta
    20521 tgctacaatt gtgggtaaag aatttaagct aatgaatttg agtgaagaat aaattaaatt
    20581 gcttcagtat tttgttaatt aatatgaata caaaaggcgt tgtgctttta taattataaa
    20641 gcgcgaggag ttcggtggat tatcgttttt cttgggtgat ttttaatatg ataaacaata
    20701 tatctgatca agcatcatca tttccaggca cacaactaaa tcaaagtgat aatttcctgg
    20761 attcactgag ggaatttttc gcgatattaa attccagtcg taagggtgag ttgtctacat
    20821 gggataccat ctacttgcat ctgatattag caataaatgc tgacagtgat ctcataaaaa
    20881 atgatgtgtt gctcgcggaa aatattccat cagcgaatta ccaatttaat acatttttta
    20941 gcaatgcttt cgagattgat gttaaaaaat atctgggtaa atcagaagat aacgaagttg
    21001 aaataaaagc gggtaatgaa agaatctcta ttggaatacg taatgtatcg aatggaaaac
    21061 ttgaaagaca acagtttctt ttcccactgg actatgagaa taaattacaa gaacaattag
    21121 ataagtattt tacgattgaa tcgcacccat tattatacag gtataccatt ggtagtaaga
    21181 ttgcgaatgt gatttttgaa aagttataca gtcgtattga ctttaacaaa gaacaatata
    21241 tatcctttat aaaggacgca tttattcatt tttatgatta tagcaggcga tatgctatta
    21301 gtgagaatat cgataaagac gctgtaacaa acaatatcgc attgatgagc actttttatg
    21361 acagtgataa tacgtcaggt gaggttttaa ataatgattt tacagaagaa gagtcatttg
    21421 aaactgcgtt agatgtagag catgcaatag tgcttggatt tgctgatgat aattttgaaa
    21481 caaaacctgt tcattatcag gatttattaa cacgatttag tgcctttcaa gataccgtgt
    21541 ttaatctgtt tcctgaaatg cattcttcgc attaccacga tatttgtagc gtaagtgtgg
    21601 atatgacaaa gggaacgcaa tgcatgattc acttgatggt gaatgaagaa gttttcatgt
    21661 ctctgcctgt tccagttgcc acgatggtac gtgaagatgc aagtaattta gtcaatctta
    21721 aaacactact caatgatggt tgtttcatta aatatagtca ttttaatgac gtggcattaa
    21781 taaagcaaaa cataagtaat ctctatttat cacacactgt tgtgaatgaa agcatattga
    21841 aaaaatgttg ttttgaaaac ggcagtttag gtgatgttaa aataacaaat agcaatgtaa
    21901 taaacagtgc attcaaaaat atttcgttta gatctgttaa aattaataac gtaaatactc
    21961 attctttaaa atttattaac tgcaattttt tcaatgttga tatgataaga gttaatctca
    22021 gcaaatgtct ttttcatgaa tgtagtatgc atggtgtcaa gataaaaccc tggttgcctg
    22081 taaaatggac aaaagaatta ataagtgatt atttgtatgg ctgtttacta tctctgtact
    22141 ctatctgtgc aagggatata tataatatga acgccgggaa taacgtgaag gttgctgcgg
    22201 atgcttttct tgaaattatt ttctctctta aaaataaata ttgtattaaa ctattaagcg
    22261 ctcaggatcg ggcgtttata tacgagtttg caagaatgat cttcgcttat attaatgaca
    22321 aaagcattga gatcttatta ctttcttgtt ttgcggcggc tgaccaaaaa gcaatccagc
    22381 ggtatagacc tcagtcacaa gatggggaag actttaggag tcatttacaa tataagttgc
    22441 ctctttccgc tcattaatgt tgttggtgca acataataag acaggtaagc ggtaccctat
    22501 aatgaaacca atatcgggtt atgcaacgga taatcgtaaa gacttaagtg ggtgtttttg
    22561 acacaatgag atgatttcct aaactgatca aaaccttctt cccaacatgg catatcagtc
    22621 cgataatgct ttagagatcg aaggcttaac aaacaaggag atcgatcatg caattttcta
    22681 caaccccaac tctggaaggc cagaccatcg ttgaatattg cggtgtggtg accggcgaag
    22741 cgattttagg tgccaatatt ttccgtgatt tctttgccgg tatccgcgat atcgttggcg
    22801 gacgttccgg tgcgtatgaa aaagaactgc gtaaagcacg ggagatcgcc tttgaggaat
    22861 taggctccca ggcgcgggcg ctgggggccg atgccgtagt tggtattgat atcgactacg
    22921 aaacggtcgg gcaaaacggc agtatgctga tggttagcgt cagcggtacg gcggtgaaaa
    22981 cgcgtcgatg agaagagtct tctggctggt cgctgccgct ctgttactgg cagggtgtgc
    23041 aggcgaaaaa ggcattgtcg agaaagaggg atatcagctt gatacccgac accaggcgca
    23101 ggcggcgtat ccgcgcatta aagtgctggt gatccactac accgcagatg attttgatag
    23161 ctcgctggcg acactgactg ataagcaggt cagctcgcat tatctgatcc ctgcggtgcc
    23221 accgcgatac aacggtaaac cgcgcatctg gcaactggtg ccggaacaag aactggcctg
    23281 gcatgcgggg attagcgcct ggcgcggggc aacgcgcctt aacgacacct ctattggcat
    23341 tgagctggaa aaccgtggct ggcaaaaatc atccggagtg aaatattttg ccccgtttga
    23401 acaggcacag attcaggcgc ttattccgct ggcgaaagat attattgccc gttatcacat
    23461 caagccggaa aacgtagtgg cacatgcgga tatcgcaccg cagcgcaaag acgatccggg
    23521 gccattattt ccctggcagc aactggcgca gcaggggatt ggtgcctggc cggatgcgca
    23581 gcgggtgaac ttttaccttg ccgggcgcgc gccgcacaca tcggtagata ccgcgtcatt
    23641 gctggagctt ttggcgcgct acggttatga cgtaaaacct gatatgacac cgcgcgagca
    23701 gcggcgcgtg attatggcat tccagatgca tttccgcccg acgttatata acggcgaagc
    23761 ggatgcagaa actcaggcga ttgccgaagc attgctggag aaatacgggc aggattagcg
    23821 cggcagtttt ccgtggtcgc gtagccaggc ggcagttttc tcgatgcctt catccagggt
    23881 gatgaccggc tgataaccta actcttcctg cgcacgcgta atatccagcg taaagtcaaa
    23941 attcaacttg gagacgccgt agtgggtcag cggcggctct tttgctgact tgcggcctaa
    24001 acgctccatg ctgcgggcga tcatatccag catcgggtaa gggacggaac gaatacgaca
    24061 gtcaatattc aactcgtcga tcagcttctg cacgatgctg cgcagtgtgc gatgctcgcc
    24121 gttggtgatg ttgtacacac gcccggaagg tagcttatcg caggcttcct ggcttgccag
    24181 ccacattgcg tgcacggcat tttcatagta ggtcatatcg accagcgcac tgccgccatg
    24241 cggtaacaga atactgccgt agtggtgcat catatgcgcc agacggggaa taaagacttt
    24301 atcgtgcggt ccgaacagac tttgtgggcg cagaatagta aagcgcgttt gtggattcgc
    24361 ctgcgaaagc atattgatca cttcttcgct ggctgctttg ctgcgggcaa actcgttggc
    24421 gaagcggtga gggcgaaaat cttctttaat atcgcgatgg tggtgataat cgaagtacag
    24481 ggagggggaa gagatatgaa taaagttacg cacaccccag gcgacagccc attcacccag
    24541 gcggcgagtg gcgcgaacgt tagccagatc gaaagcctgt tgtgtccccc agggtgaggt
    24601 aaagctggag cagtgccaca gcgtatcaat gcccgcgagc atcactttag cttgtgatga
    24661 aaccagctcg gtcagatccg tcggaacaaa ctctgcgccc attttttcca gcaatttacc
    24721 cattgcttcg ttgcgaccgc tcgcacgcac gctgatgtct ttctggcata aaaactctac
    24781 cgcgtttcga cctaagccgc tggtggcgcc ggtaaccagt accttcatat caatccactg
    24841 ttgttgagaa aatgacgtgc gcattcttcc gtgatttccc ccatgatgca atgggaaaca
    24901 tgaaagaata acgcaggttt tgtcgattaa tctgtgcttt gttctgccag tctggcgatt
    24961 tgttttgcca ttccgcgaaa aataaacaga tgcgcgggga tcatcaataa ccagtaaaac
    25021 agccccggca taccgtgcgg atgccagaaa gcgcggacat cgatagtacg atagtcacct
    25081 ttatcttcca gggtaaaaca cagtcgtccc agccccggcg ctttcatgcc aaataacaac
    25141 gtaagttgct ttttcggttc aacgacaatc actttccagc tatccaccgc atcgccggtc
    25201 tgtaaatatt cgcgctccgg gcggcctttc gccagattat gaccgatcgc gcggtccatc
    25261 aacgcccgtg tctgccacaa aatattgcca aagaaataac gctctttacc gccgatttgg
    25321 ttcaccacca gccataaagc cgcaaggctg gcggacgttt taacggtaaa ccccgcctgt
    25381 ttggcgaaat aaccgtactc cggtcgccag cgggcaaagg cctgagcgtc gtagccccag
    25441 tcgctggagt tgaccagttt ttcttcctct ttcaacgtgc tacgtaccgc gtcatcgaaa
    25501 gcgatcagcc gttgtgggat gagtgcacgt agcgcggtat catctgccag cagatcgtgt
    25561 ttcagcccct gaatcaacgc cctggcggtg gtgggcggta cggaagtaat cacattgaga
    25621 aaccacaccg aaatccagcg ggtggggagg gggatgggga tcaaccagcg gcgcttaccg
    25681 ctcaccgcca taaaatgttc aaactgttgc tgataactga gcacctctgg tccggcggct
    25741 tcgaagatgc ggtgttcgtt ggctggatgg tctaacagcg ccaccagata gtgcagcaag
    25801 ttttccagcg cgatgggcgt ggtgcgtgaa cgtacccagc gtggcggcgt taacactggc
    25861 aggttgtaga ccatatcgcg catgacttcg aacgccgctg aacctgcgcc aacgataatt
    25921 ccggcccgaa gttcggtcac tggtacattc gcttcacgaa gaatgtccgc cgtagcctga
    25981 cgagcacgca gatgatccga ctgctcatgt ggcggggcct gcaacgaact gagaaagatt
    26041 aattgcttaa ctggtacttc acgtagcgca tcgcggacgt tgagagccac ctggcgctcc
    26101 tgagcgataa aatcgccgcc ttcgcccatg ctgtgcacca gaaaatagac ggtatcgata
    26161 tcctgcaaca gggccggaag gttatccggc cagctgagat cgactttatg gcaactgacg
    26221 tttgccagtt gcagctttgc aagcctgtcg acatgacgtg ccgccgccag gatctgatgc
    26281 ccttgctggc tgagtgtgcg caccagatgc tgaccaatgt agccactggc accgagaact
    26341 aaaatgcgtt gcggcacgtc tctctcctta acgcgccagg aatgcgcgcc agtgggcggc
    26401 gatttccgcc agttgttcgc gcgagacgtc aagatgcgtc accaggcgga caatcggcga
    26461 ggcgttaatc agcacgtttc tcgctttcat gtattcgcct aacgcggcag cattttcttc
    26521 cccgacgcga acaaacagca tattagtgtc ctgacgcatc acatccgcgc ctgcttcacg
    26581 cagttgctcc gccatccagg cggcgttgtc gtgatcttcc tgcaaccgtg cgacgttatt
    26641 tttcagggca tacatcccgg cggcagccag aatcctggac tgacgcatcc cgccaccggc
    26701 cattttccgc cagcggatgg cacgtttaat gtaatcacga ttaccgacga gtaatgaacc
    26761 gactggcgtc ccaagacctt tcgacaggca aatggtgaac gaatcacaat attgcgtgat
    26821 ctctttcagt tcgcagccgt aagccaccac ggcattaaag atgcgcgcac cgtcaacatg
    26881 cagcgccaga ttgcgctcgc gggtaaattc ccatgcttct ttcaggtatt cacgcggcag
    26941 cactttgccg ttgtgggtgt tttccagact gagtaatttg gtgcgggcga aatggatatc
    27001 gtcgggtttg attttcatcg ccactttatc cagcggtagc gtgccgtcgg cagccgcgtc
    27061 gatgggttgc ggctgaatac tgcccagcac cgctgcgcca ccggcttcaa acagatagtt
    27121 atgcgcggcc tgaccgacaa tatactcttc gccacgttcg cagtgactga gcagagcgac
    27181 caggttggcc tgagtgccgg taggcagaaa aatggcggct tctttaccgg aaagctctgc
    27241 tgcatagtcc tgcagagcat taacggtagg gtcgtctccg taaacgtcgt ccccaaccgg
    27301 ggcggccatc atcgcttcga gcatggcgcg gctcggtcgg gtaacggtat cactgcgtaa
    27361 atcaatcatg gcatgtcctt attatgacgg gaaatgccac cctttttacc ttagccagtt
    27421 cgttttcgcc agttcgatca cttcatcgcc gcgtccgctg ataattgcgc gcaacatata
    27481 caggctaaaa cctttggcct gttcgagttt gatctgcggt ggaatggcta attcttcttt
    27541 ggcgaccacc acatccacca acaccggacc gtcgatagag aaggcgcgtt gcagggcttc
    27601 atcaacttca gaggcttttt ctacacggat acccgtaatg ccgcacgctt cggcaatgcg
    27661 ggcaaatttg gtgtcgtgca gctcagtacc gtctgtcagg taacctccgg ccttcatctc
    27721 catcgccaca aagcccagca cgctgttatt aaagacgata attttcaccg gcagtttcat
    27781 ctgcactact gagaggaaat cgcccatcaa catgctaaaa ccgccatcgc cgcacatggc
    27841 gaccacctga cgttccggct cggttgcctg cgcacccaac gcctgcggca tggcgttagc
    27901 catcgaaccg tggttaaacg aacctaacag gcgacgcttg ccgttcattt tcagataacg
    27961 tgccgcccat accgttggcg tgccgacgtc acaggtaaag atggcgtcat cggcggcaaa
    28021 atgactaatt tgctgcgcca gatattgcgg gtgaatggct ttctcgctcg gtttagctaa
    28081 atcgtccagc cctttgcggg catcgcggta atcttccagc gctttatcca gaaacttgcg
    28141 atcggttttt tcttccacca gtggcagcaa tgcgcgcagg gttgatttga tatcgccgac
    28201 cagcgccata tctaccttgc tatgcgcgcc gatgctggct ggattgatat caatctgaat
    28261 aattttggca tcggtcgggt agaacgcacg gtaggggaac tgagtaccga gtagcactaa
    28321 cgtgtcggcg ttcatcatgg tatggaaacc tgacgagaat ccgattaacc cggtcattcc
    28381 aacatcatac ggattatcgt attcgacatg ctctttaccg cgtagggcat gaacaatagg
    28441 tgctttaatt ttcccggcaa actcaactaa ctctttatgc gcccccgcgc agccgctgcc
    28501 acacatcagg gcgatattgc tggaataacg cagcagttgc gccagtttgc gtaactcttc
    28561 ttcttccggc gtcacgactg gctgtggcgc atgataccag tgcgtggttg ccccttctgg
    28621 cgcaggtttt aacgccacgt cgcccggtaa cacaacaacg gaaacgccac ggttaagcac
    28681 cgctttgcgc atagcaattg ccagcacttg tgggatctgc tccgggctgg aaaccagctc
    28741 gcaatagtga ctacattcgc ggaatagctc ttgtgggtgg gtttcctgga aatagccgct
    28801 gccaatttcg ctggagggaa tatgagcggc aatcgccagt accggaacgt tattgcggtg
    28861 gcaatcgaat aggccgttga tcaagtgcag gttgcctggg ccgcacgatc cggcacagac
    28921 cgccagttct cctgaaagtt gtgcttcagc gccagcggca aacgccgcca cttcttcatg
    28981 gcgggtggac atccactcga tggtgcccat gcgattaaga ctgtcgctaa gaccgttcag
    29041 agagtcgccc gtgactcccc agatgcgttt cacgcctgcc gattcgagtg ttttggcgat
    29101 ataagctgca accgtttgtt tcatggttct ccatctcctg aatgtgataa cggtaacaag
    29161 tttagttcat ctgacggagg gggaagggat gggagagaaa ggaggcacta acggttaaat
    29221 agcccgatga aaggaatatc atcgggcata aggcgattat gcgagaacca aatccccctg
    29281 cggatggcag gagcaggcca gtacgtaacc ttcagcgatt tcggcgtcgg tcagcgtcat
    29341 tgtactgctc accgtatatt caccggaaac cacttttgtc ttacagcagc cgcaaacacc
    29401 cgcacggcag gcagcgacaa ccggaacgtt attgctttcc agcgcctcca gtagcgtggt
    29461 gccaaccggg gcgtaaaatt ctcgtgccgg ttgcagtttg gtgaatttca gaccgctggt
    29521 cgccgcttct gctactgggg tgaagaattt ctctttaaag aaacgcgtca cgccgagcgc
    29581 tttcacttcc tgctctaccc aatccatata cggagccggg ccgcaggtca tcacggtacg
    29641 tgaagctaag tcaggtacac ctgccagcag ttcgcgagtg agacgaccag cgataaagcc
    29701 ttcggtaacg ttattttctg ccaccagcgt taccggatag ttacgccact catcggcgaa
    29761 aataacatcc tgcggcgtac gcacgttgta gatcacccgc acatcggcct gtggacggtt
    29821 cttcgcaagc cagcgacgca tcgacataat cggcgtgacg ccgcagcctg ccgccagcaa
    29881 caggaattta tcttctgctt tatcgtcgca ggtaaattcc cccatcgcgt ccgaaagcca
    29941 gagataatca ccgcgtttta catcgcgcgt cagccactgg gagccgacac cgtcatcaat
    30001 ccgccgcacg gtcagggtga tatattcact cacgcctggc gtggaggaaa tggtgtaagc
    30061 acgcagcgtt tccgctgagt ttcgcacgct gaccagtgca tattgcccgg cgcgatatgg
    30121 gtagtgatcg tggcaaatca gggaaatcgt ccacacatcc ggcgtttctt gcgtaatgtg
    30181 atgaacctgc atccgccacg ggcattgatt cgttggcatc gtcatcgaca aactccttac
    30241 gcgctcaaca gttgcttcat gtcttcttca acagtggtga tagaacgcag gccgaatttc
    30301 tcgttcagca ccgccagcag gtctggtgtc aggaaaccag gtgcagtcgg gccggtgacg
    30361 atatttttca cgcccagaga aagcagcgtc agcagaatga caatcgcttt ctgttcaaac
    30421 caggagagca ccagcgacag cggcagatcg ttcacaccac agcccagttt ctctgccaga
    30481 gtgacagcca gaataattgc tgagtaagca tcgttacatt gacctgcatc taccagacgc
    30541 ggcagacctt cgatatcgcc aaactcaagt ttgttaaagc gatatttacc acaggcgagg
    30601 gtcaggatca ggcagtcatc cggcacgctg gtggcgaaat cggtgaagta gtggcgctcg
    30661 ccgcgtgcgc cgtcacagcc accaagcagg aagatatgac gcagtttttc acggctaacc
    30721 agatcaatca gcgtatcggc tgcaccaagc agcgtctggc gaccaaagcc cacggtgatc
    30781 aggtgaggaa tttcgctgta cgggaagccc gccatttgtt gcgcctgggc gatgaccgca
    30841 gagaaatctt cgccatccag atgacgcacg ccaggccagc caacgatgct gcgggtccag
    30901 atatgatcgt cataagcgcc tacggttggg tcgatgatgc agttcgaggt catcacgatg
    30961 gggccaggga aacgagcgaa ctccacttgc tgattctgcc agccgctgcc gtagttaccg
    31021 accagatgct tgaatttacg cagctccggg tagccatgcg caggcagcat ttcgccgtgg
    31081 gtgtagacat taacgcccgt gccttcggtc tgttccagca ggttgtagag atctttgaga
    31141 tcgtgaccgg aaatcagaat gcatttaccc gccgtcgctt tgacgttgac ctgggttggc
    31201 gtcgggtgac cgtatttacc ggtttcgcct gcatccagaa tgctcatcac tttgaagttc
    31261 atctggccga tttccattga acactcaaga agcgcgttca tatcggcagg ccaggtcccc
    31321 agccacgcca tgattttatg gtactgggca taaatatcgt tgtcgtattg accgagaaca
    31381 tgcgcgtgtt ccatataggc cgccgcacct ttcaggccat acaggcacag cagacgcagg
    31441 ccgagaatgt tttcgccaat cgccgcttta tctttgttag gggtaaattc tgctgcctga
    31501 cgttgcagct cgccgagatc atcgctcacc agttgcaggt cagccatcgg gttatcgacg
    31561 cgcgcgttgg catctacagc caggcattgt gctttcagcg cctcgcgcaa ggcaatcgct
    31621 tcacgagcgt agccgacaat acgcggagaa tcgaagttaa cgttggtcag ggttgagaaa
    31681 aaggcacgtg gcgcgaagct gtcaacatcg tggttgatga tgccgtattc acgcgctttt
    31741 accgcccagg cagaaagccc ttgcagcgcc gcgatgagta aatcctgaag gtcagaagtt
    31801 tccgccgttt taccacacat cccctgcgcg tatgagcagc cgtttcctgc cggagtacgg
    31861 atagtttgtt cacattgcac acaaaacatg atcacacctt ttaaagttat atttaatata
    31921 catgtttaag gttaagacgc ttaacgcggg gataaaaggg atttttcatg caactttaag
    31981 ggagattgat ttagcgcaat tttggcggca gggatctacc gccagagagg tattacgcag
    32041 agaaaaaggc gatgaggatc ggcactaaca ggctaagaat aaaaccgtga acaattgccg
    32101 ccgggaccat atccagcccg ccagtacgtt gaagaacggg cagggtgaaa tccattgatg
    32161 tggcaccgca taagcccagt gcagtagagc ggctgcggcg aatcagccca gggatcaaca
    32221 taatagcaat cagttcacgg gccagatcat taaaaaacgc cgcgctcccg attaccggac
    32281 caaaagattc ggtcaataaa ataccggaaa gagaatacca gccgaaaccg gaggccattg
    32341 ccagcgcggt attgatgggg agatcaagaa taaaggcgtt aattaaacca ccaattaatg
    32401 aactgacaac caccaccacg gcgacaatca ttccccggcg attaaggaca atctgcttta
    32461 aggtcatgcc attattgcgc aactgaatac caacgaggaa aagtagcaaa attaacgtgt
    32521 attcactggc ttcggtcgcg tgttgtaaga aagccagtcc actcagacca atggcaaaac
    32581 caatcactac tacgccgcac agttttagcg actccagcgc catcgcaata cgcgacggga
    32641 gtttttcttg ctgatggtgg ttgcgccacg gcaggcctcg ctccagccac atcagggcgg
    32701 caatattaca cagtaaaata acggtaatac tgacggcaga ataatgcaga atcgccaaca
    32761 ggttactggc gaggttatcg agaaacgcca gactgatacc cataaaaaag agaataaggt
    32821 aaaccatcca gcttaatagc tgattaataa cttttaacgc agcttgttgg cgaagcggaa
    32881 tgaggtaacc cacaatcagg ggaaccagaa tgattaacag cccagaaaac atgaaaatcc
    32941 agcccttgca aagaagaagt cgaaatgcgc gatgacacac tactgaaagc ggaaggacga
    33001 gtaaagttgc aattaaaagg aaatgttatg catcagaggg ggatttttaa tcctatctga
    33061 gtgtttagca ttgttgcgct cctttcatgt gcaatgccat ataaagccac tggcggaggg
    33121 agcgcgcagg gggcagccag tcgccgccgc cccctg
//
LOCUS       DBKEKI010000035        33022 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.035, whole genome shotgun sequence.
ACCESSION   DBKEKI010000035 DBKEKI010000000
VERSION     DBKEKI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.035"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..399
                     /locus_tag="ACUQPX_001607"
     CDS             <1..399
                     /locus_tag="ACUQPX_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HHV9056353.1"
                     /translation="NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNS
                     AMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYS
                     QLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            396..1484
                     /locus_tag="ACUQPX_001608"
                     /pseudo
     CDS             396..1484
                     /locus_tag="ACUQPX_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
     gene            complement(1572..2870)
                     /gene="exuT"
                     /locus_tag="ACUQPX_001609"
     CDS             complement(1572..2870)
                     /gene="exuT"
                     /locus_tag="ACUQPX_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HHV9056354.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            3353..4765
                     /gene="uxaC"
                     /locus_tag="ACUQPX_001610"
     CDS             3353..4765
                     /gene="uxaC"
                     /locus_tag="ACUQPX_001610"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HHV9056355.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            4780..6267
                     /gene="uxaA"
                     /locus_tag="ACUQPX_001611"
     CDS             4780..6267
                     /gene="uxaA"
                     /locus_tag="ACUQPX_001611"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HHV9056356.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYDLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            6350..6901
                     /locus_tag="ACUQPX_001612"
     CDS             6350..6901
                     /locus_tag="ACUQPX_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HHV9056357.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(6906..8150)
                     /gene="sstT"
                     /locus_tag="ACUQPX_001613"
     CDS             complement(6906..8150)
                     /gene="sstT"
                     /locus_tag="ACUQPX_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HHV9056358.1"
                     /translation="MTTQRSPGLFRRLAHGSMVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(8746..9711)
                     /gene="alx"
                     /locus_tag="ACUQPX_001614"
     CDS             complement(8746..9711)
                     /gene="alx"
                     /locus_tag="ACUQPX_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HHV9056359.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLTLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(9982..10980)
                     /locus_tag="ACUQPX_001615"
     CDS             complement(9982..10980)
                     /locus_tag="ACUQPX_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HHV9056360.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGALVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(11059..11751)
                     /locus_tag="ACUQPX_001616"
     CDS             complement(11059..11751)
                     /locus_tag="ACUQPX_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SanA/YdcF family protein"
                     /protein_id="HHV9056361.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGHKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(11828..12331)
                     /locus_tag="ACUQPX_001617"
     CDS             complement(11828..12331)
                     /locus_tag="ACUQPX_001617"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HHV9056362.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12416..13552
                     /gene="rlmG"
                     /locus_tag="ACUQPX_001618"
     CDS             12416..13552
                     /gene="rlmG"
                     /locus_tag="ACUQPX_001618"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HHV9056363.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            13837..14151
                     /gene="higB"
                     /locus_tag="ACUQPX_001619"
     CDS             13837..14151
                     /gene="higB"
                     /locus_tag="ACUQPX_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HHV9056364.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            14148..14564
                     /gene="higA"
                     /locus_tag="ACUQPX_001620"
     CDS             14148..14564
                     /gene="higA"
                     /locus_tag="ACUQPX_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HHV9056365.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(14609..16627)
                     /gene="fadH"
                     /locus_tag="ACUQPX_001621"
     CDS             complement(14609..16627)
                     /gene="fadH"
                     /locus_tag="ACUQPX_001621"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HHV9056366.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKITLQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(16853..19204)
                     /gene="ygjK"
                     /locus_tag="ACUQPX_001622"
     CDS             complement(16853..19204)
                     /gene="ygjK"
                     /locus_tag="ACUQPX_001622"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HHV9056367.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMVHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQANYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(19221..20292)
                     /gene="ygjJ"
                     /locus_tag="ACUQPX_001623"
                     /pseudo
     CDS             complement(19221..20292)
                     /gene="ygjJ"
                     /locus_tag="ACUQPX_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(20426..21859)
                     /locus_tag="ACUQPX_001624"
     CDS             complement(20426..21859)
                     /locus_tag="ACUQPX_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHV9056368.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(21922..22371)
                     /locus_tag="ACUQPX_001625"
     CDS             complement(21922..22371)
                     /locus_tag="ACUQPX_001625"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HHV9056369.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(22368..25460)
                     /gene="ebgA"
                     /locus_tag="ACUQPX_001626"
     CDS             complement(22368..25460)
                     /gene="ebgA"
                     /locus_tag="ACUQPX_001626"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HHV9056370.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSH
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(25644..26627)
                     /gene="ebgR"
                     /locus_tag="ACUQPX_001627"
     CDS             complement(25644..26627)
                     /gene="ebgR"
                     /locus_tag="ACUQPX_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HHV9056371.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            26846..27177
                     /locus_tag="ACUQPX_001628"
                     /pseudo
     CDS             26846..27177
                     /locus_tag="ACUQPX_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(27219..28598)
                     /gene="ygjG"
                     /locus_tag="ACUQPX_001629"
     CDS             complement(27219..28598)
                     /gene="ygjG"
                     /locus_tag="ACUQPX_001629"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HHV9056372.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            29016..30536
                     /gene="aer"
                     /locus_tag="ACUQPX_001630"
     CDS             29016..30536
                     /gene="aer"
                     /locus_tag="ACUQPX_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HHV9056373.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSSFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQFGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(30584..31207)
                     /gene="nfeR"
                     /locus_tag="ACUQPX_001631"
                     /gene_synonym="yqjI"
     CDS             complement(30584..31207)
                     /gene="nfeR"
                     /locus_tag="ACUQPX_001631"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HHV9056374.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            31484..32248
                     /gene="nfeF"
                     /locus_tag="ACUQPX_001632"
                     /gene_synonym="yqjH"
     CDS             31484..32248
                     /gene="nfeF"
                     /locus_tag="ACUQPX_001632"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HHV9056375.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEKYDPQRVRAAAYWHAK"
     gene            complement(32302..32377)
                     /locus_tag="ACUQPX_001633"
     tRNA            complement(32302..32377)
                     /locus_tag="ACUQPX_001633"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32342..32344),aa:Ile,seq:cat)
     gene            32502..33008
                     /gene="mug"
                     /locus_tag="ACUQPX_001634"
     CDS             32502..33008
                     /gene="mug"
                     /locus_tag="ACUQPX_001634"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HHV9056376.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT         8032 a         8562 c         8676 g         7752 t
ORIGIN      
        1 aatcgtgaat taatgttgtt gcctggcaag ctgcgctatc gcgaagtgtc tgccagcttt
       61 aactacaact atatcggtcg tttattactg ccggcagcgg tgaaaaaatt cccgattgtg
      121 gggctgaaca gcgccatgtt actggtagct gaagatggcg gatttacact tgaaattaac
      181 ggcagcgaaa aagagctcta tctgctttcc gggcagcaat tccttaagtg tccactgagt
      241 gttgtaaaga aacgcgccag cattcgttac agcggagatg ttacttgtag tgtggtgact
      301 tattcacaat taccggagtc gattcaggtt caggcacagt tgaaacagcc taaattacgt
      361 ggaaacgttc agacggcgca aagggaggtt gcaccatgag aaatcgattg attgcggcga
      421 tattgggctt gtttggcacg ctcactggcg ttcaggcagc tcctgacgtg accagtgaaa
      481 ttacgtatga tttggcatct ggtagagcgg attattactt ctggaaggat gaagcgtcgg
      541 caggaaataa tggatatatg tggtatgaat gttcgtatcc tgacctccaa caaacctgta
      601 cagctaatgg aaatatatcg acagtacaaa tctatttaac tgaacaacgc agtgggatgc
      661 gttggccgat aaaactcaaa ggatttaaaa cagccattgt aagtagcgat gaagcgccgc
      721 taggatgcaa ggggggcaaa gggcttcaga cgaatcttaa ggattctaat agatcttcat
      781 gtacagaaga tggtcaacat tattatatat acgatacaaa gtttcttacg ctctaccttg
      841 agcagacaga gatgaagaat ttgccgattg gtggcgtctg gaaggggaaa gttaaattac
      901 attcgaacag cccggcccag gactatttcg caaatattac cctgaatacg ctcgacccca
      961 accatattga cgtgttcttc ccggagttcg cccacgccac gccaagggtg cagttagact
     1021 tgcatccaac aggaagcgtt aacggcagca actacgcgca agatctgacc atgttggaca
     1081 tgtgcctgta cgatggtttt aacggtaatg ccatcagtta tgaaatcatg ctcaaagatg
     1141 aagggcgacc agctgcaggg cgcagagacg gttacttctc tatctatcgt cagggaggga
     1201 ccaccaccga cgagggagaa cgcattgatt accgggtcaa aatgtacaac ccggaaaccg
     1261 gtgggcaaat tgatgtgcgc aataatgaaa atatggtctg aaacagcatt aacctgaaac
     1321 gtgtgcgtcc ggtggtactg cccggtattc gctatgccgt gatgtgtgtg ccgacaccct
     1381 tgactctggc agtcgataaa ttcagcgtga tggataaaca ggccgggtac tacatgggca
     1441 aattgtcagt aatctttacg ccttccttgc caaccatcaa ttaaccctgc aaaccggaca
     1501 aacgcttttt tctatataaa cgacgaagcc gcctgataag gcggcttcgt cgttataact
     1561 tacgttaaac gttaatgttg cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc
     1621 gccggtttgt tctgcaatac ggtccagata accagcgcac ccagcaggtc aaatactgcc
     1681 agaactgcga acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca
     1741 aacagcgtac ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg
     1801 ttacgaccga acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgggca
     1861 aaaccaccga tacacagcag cataattgcg acatacgggt tggtgaacag accgatcata
     1921 cccgggccaa tcatcagcac agcacccagc gttacgacca tcttacggga aacgatcagg
     1981 ttcacaccaa accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg
     2041 tcagcaaaca acatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa
     2101 actttaaaca tgaacagcgg gatccacgcg ttaaaagtac cccaggccgg ttctgccagg
     2161 aaacgcggca gtgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt
     2221 ttcttcgccg tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct
     2281 tcatcggtca gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc
     2341 cagataaagc tcaatgcacc ggagatgata aatgccatct gccagctgtg catcacgatt
     2401 gcccatacca ccagcggcgg cgcaatcatc gcaccaatcg aagaacctac gttaaagtag
     2461 cctactgcga tggaacgctc tttcgccggg aaccattcgg agctggcttt cagacccgcc
     2521 gggatcatcg ctgcttccgc ggcaccgacc gcaccacgag caacagccag gccaccccag
     2581 ctacctgcca gcgcggttgc accacagaac acggcccaca gcacagcaaa cattgcataa
     2641 ccgattttcg tacccagcac atccagtaca taacctgcta ccggttgcat gaccgtataa
     2701 gcagcagaat aggctgcgat gatataggaa tactgttggg tggagatgtt taactcttcc
     2761 atcagagttg gcgcagctgc cgccacagtg ttacgcgtca ggtaaccaag cacggtgccg
     2821 agcgtcacca gtgcgatcat ataccaacgt aaccctttaa ttttacgcat gtaaaacctc
     2881 atcgttttgt tatatccgca ggaagcgcgg ccatcatccc gccagaggct gaagatgtta
     2941 gttacgaccg ggtaaccgaa aaaaaaccgg cacgccccga acgttgccat gaaagtttcg
     3001 tgctaattcg gcttccgtac cggttaaccc gattcatcta acaaatgaat gaatcggcaa
     3061 tttgcattcc gtcgacttat gatttgcgac ggcagaaaga taacttgtca tacaacctta
     3121 aaaggtgaga gccatcacaa atgtgcgaat atttgtaggg acattacctg acgacagcga
     3181 ggccagtact ggcgcggcct gcagcgagat ttaccacttt gagagtaatt tttttaacta
     3241 cgtttattga tctaactcac gaaaatatct tcggactctg gaaattggtg tgataacttt
     3301 gtcagcatcg caccataagc aagctagctc actcgttgag aggaagacga aaatgactcc
     3361 gtttatgact gaagatttcc tgttagatac cgaatttgcc cgccgtctgt atcacgacta
     3421 cgcaaaagac cagccgattt tcgattacca ttgccatttg ccgccgcagc agattgcgga
     3481 agactatcgt tttaaaaacc tgtatgacat ctggttgaag ggcgatcact acaaatggcg
     3541 cgctatgcgt accaacggtg tggctgagcg tctgtgtact ggtgatgcgt ctgaccgtga
     3601 aaaatttgac gcctgggcgg cgactgttcc gcacactatc ggcaacccgt tataccactg
     3661 gacgcacctc gaactgcgtc gtccgttcgg tatcactggc aaattgcttt ctccgtcaac
     3721 tgccgatgaa atctggaacg aatgtaacga actgctggcg caggataact tctccgcgcg
     3781 cggcatcatg cagcagatga acgtgaaaat ggtcggcacc accgatgacc cgatcgattc
     3841 tctggagcat cacgcagaga tcgccaaaga cggctctttc accatcaaag tgctgccgag
     3901 ctggcgtccg gacaaagcct tcaatatcga acaggcgacc tttaacgact acatggcgaa
     3961 gctgggcgaa gtttccgata ccgacattcg ccgctttgct gacctgcaaa ctgccttgac
     4021 caaacgtctg gatcacttcg ctgctcacgg ctgtaaagtg tctgaccatg cgctggacgt
     4081 ggtgatgttt gctgaagcga acgaagcgga actggacagc attctggcgc gccgtctggc
     4141 tggcgaaacc ctgagcgagc acgaagtggc acagttcaaa actgcggtac tggtgttcct
     4201 tggtgccgaa tatgcacgtc gcggctgggt acagcagtac cacattggcg cgctgcgtaa
     4261 taacaacctg cgtcagttca aactgctggg gccggatgta ggctttgact ccatcaacga
     4321 ccgtccgatg gcggaagagt tgtctaagct gctgagcaag cagaacgaag aaaacctgct
     4381 gccgaaaacc attctctact gcctgaaccc gcgcgataac gaagtgctgg gcaccatgat
     4441 cggtaacttc cagggcgaag gtatgccggg caaaatgcag ttcggttccg gctggtggtt
     4501 taacgatcag aaagacggta tggaacgtca gatgacccaa ctggcgcagc tcggtctgct
     4561 gagccgcttt gtcggtatgc tgactgacag ccgtagcttc ctgtcataca cccgtcacga
     4621 atacttccgc cgcattctgt gccagatgat cggtcgctgg gtggaagcgg gcgaagcacc
     4681 ggcggacatc aacctgctgg gcgagatggt gaaaaatatt tgctttaaca atgcgcgtga
     4741 ctacttcgcc attgaactga actaaggtct ggggttgata tgcaatacat caagatccat
     4801 gcgctggata acgtcgcggt cgctttagca gatttggctg aaggcacaga agtcagtgtc
     4861 gataaccaga ctgttacgct gcgccaggat gttgctcgtg gacataaatt tgcgttaacg
     4921 gatatcgcaa aaggggccaa cgtcatcaaa tatgacctgc cgattggtta tgcattggcg
     4981 gatattgcgg cgggggaaca cgtccacgcc cacaatacgc gcacgaatct gagcgatctg
     5041 gatcagtatc gctatcaacc tgattttcag gatctgcctg cgcaagcggc agatcgtgaa
     5101 gtgcagatct atcgtcgcgc taacggcgat gtcggggtgc gtaacgagct gtggatcctg
     5161 ccaaccgtgg gctgtgtcaa cggcatcgcg cggcaaatcc agaaccgttt cctgaaagag
     5221 accaacaacg ccgaaggtac cgacggcgtg ttcctcttca gccacaccta cggctgctca
     5281 caactgggcg acgatcacat caacacccgc accatgctgc aaaacatggt gcgccacccg
     5341 aacgcgggcg cagtgctggt gattggtctg ggctgtgaaa acaaccaggt tgccgcattc
     5401 cgtgaaacgt tgggcgatat cgatcctgaa cgcgttcatt tcatgatctg ccaacagcag
     5461 gatgatgaga tcgaagccgg gatcgagcat ttgcatcagc tgtataacgt gatgcgcaac
     5521 gataaacgcg agccaggcaa actcagcgaa ctgaagtttg gtctggagtg cggtggttct
     5581 gacggtcttt ctggtattac tgctaacccg atgctggggc gtttctctga ctacgtgatt
     5641 gctaacggcg gtactaccgt actgaccgaa gtgccggaga tgtttggcgc agagcagttg
     5701 ctgatggacc attgccgcga cgaagcaacg tttgaaaaac tggtcaccat ggtcaacgac
     5761 ttcaaacagt actttattgc ccatgatcag ccgatctacg aaaacccatc accggggaac
     5821 aaagcgggcg gtatcaccac gctggaagac aaatcacttg gctgtaccca gaaagcgggt
     5881 tccagtgtcg tggttgacgt gctgcgttac ggcgagcgtc tgaaaacgcc agggctgaac
     5941 ttgttaagtg cgccgggtaa cgatgccgta gcgaccagcg ccctggcggg tgcgggctgc
     6001 catatggtgc tgttcagtac tggtcgcggc acgccatacg gtggatttgt gccgacggtg
     6061 aaaatcgcca ccaacagtga actggcggcg aagaaaaaac actggatcga ctttgacgcg
     6121 ggtcagctga tccacggtaa agcgatgccg cagttgctgg aagaatttat cgacaccatc
     6181 gttgagtttg ccaacggtaa gcaaacctgc aacgagcgta acgacttccg cgaactggcg
     6241 atcttcaaaa gcggcgtaac gctataaagt cgaaatgagt tgttaataaa aaacggcgtt
     6301 tcataatgtg aagcgccgtt tgcttttcca tacctgctaa ggatttatca tgaccgcgta
     6361 ttggctggcc cagggcgtgg gtgtcatcgc ctttctgatt ggtatcacaa catttttcaa
     6421 tcgtgacgaa cgtcgcttca aaaagcagct ttcggtctat agcgccgtta ttggcgtaca
     6481 tttttttctt ctgggcacct atcccgctgg tgccagtgcc atccttaatg ccattcgtac
     6541 attgattacc ttacgcacgc gcagcttatg ggtaatggcg atttttattg tgctgactgg
     6601 cggaattggc ctcgcgaagt tccatcatcc tgtcgaacta ttgccggtta tcggtacgat
     6661 tgtcagtacc tgggcgctgt tccgctgtaa agggctcacc atgcgctgcg tgatgtggtt
     6721 ttcaacgtgt tgctgggtga ttcacaactt ctgggcgggg tcgataggcg gcacgatgat
     6781 tgagggtagt tttctgctta tgaatggcct gaatatcatt cgtttctggc ggatgcagaa
     6841 aaggggaatt gatccgttta aagtagagaa aacccctccc gccatagacg aaaggggtta
     6901 aacaattaat tacgcagggc gctatttgcc agacgatcgt cttccgcctg gcaagctgcc
     6961 gcagtgaaca gcacgtcagt tgaagagttc agcgcggttt cgcaagagtc ctgcaatacg
     7021 ccgataataa agccgacggc aaccacctgc atggcgatat cgttcgaaat accgaacata
     7081 ttacaggcca gtgggatcag cagcaaagaa ccccccgcca cgccggatgc gccacaggca
     7141 cacagagaag ccactacgct caacagcagc gctgtgggca gatcgaccgg aatacccagc
     7201 gtattaaccg cagccagcgt caacacggta atggtgattg ctgcgcccgc catattgatg
     7261 gtggctccca gcggaataga aactgaatag gtatcgcgat ccagattcag cttttcacac
     7321 agcgccatat tcaccggaat gttcgctgca gagctgcggg tgaagaaggc atacacaccg
     7381 ctttcgcgca ggcacagcag caccagcggg aacgggttac gacgaatttt ccaccacacc
     7441 agcaatgggt taaccaccag cgccaccagt aacatacagc caaccagcac gaccagcagt
     7501 tgcgcgtagc cccacagtgt ggagaaaccg gtggttgcca gggtagaaga aaccagccca
     7561 aaaataccga tcggtgcgaa gcgaatgacc agtttcacca taaaggtaac ggcattcgac
     7621 atatcattga ccaggttttt ggtggtctcg ttaccgtgac gcagtgcgaa gccgaggccg
     7681 atcgcccaca ccagaatccc gatgtagtta cctttcagca gggcgtcgat ggggttggaa
     7741 accatgctca ttaccagtcc gcgcatcact tcgacaatgc ctgacggcgg cgaaatatca
     7801 cccgcgctac tggacaagtg cagggtagaa gggaaggcaa agctgaagac tactgcggcc
     7861 agagctgcag agaaggtgcc cagcagatag aggaacaaaa taggacggat attggttttc
     7921 tgcccgtgct ggtggttagc aatagatgcc attaccagca tcaacaccag gatgggggca
     7981 acagctttca gtgcgccgac gaacaaagta cctaacagac caacagcttc cgccgcgggt
     8041 tttgagatcc atgccagaag aatccccaga acaaggccga ccaggatttg ttttaccatg
     8101 ctgccatgag ccagacgccg gaatagcccc ggtgaacgtt gcgtagtcat ttttcgatcc
     8161 tttcattgtg ttgtcgcaca tccctggtgc gttctgtcga cgcatctttc aggatgtaac
     8221 aaagtgtttg caaggtcgag tataaggaaa gttacagttt cgggaagcga aaaatgctgg
     8281 attttaaaga gaggtcatgt tttgcaactt aatggttgca tttatttaac ataaacagag
     8341 ggaaggactg gctactaagg ctgattagat aacatatggg caaaactatt tggaatatga
     8401 gaacggttgc gctcctttaa cgtgttatgc catataaagt cactggcgca gggagcgcgc
     8461 agggggcggc caatcgccgc cgccccctgc acccccaggc tctggcgaac aaaatcgccg
     8521 ctgcgcggtt ccctcagcct atcccttgcg gctaccgggt cgggcgcaag gtaacgtccc
     8581 tgtaagatgc gccctcgccc cggcctccag ggaacgcttc ggcgattttg acgccaccaa
     8641 cacccgtgcg gtctttttgt aaagagctat aagccgatta cataattaat agctcacctt
     8701 tgtctggaat gagttatctc taggaatggc ttaatataca gcgaatcatt ctccccgctg
     8761 cttatcatgc cgataattca cccaggcgtt gataataaac gtcatcacca gaatgccaaa
     8821 caccacgccc agcgagacgg cgattggaat atggtagaag tcgacaatca gcatcttgat
     8881 accgataaac accagaatca ccgccaggcc atatttgagc atcgagaaac gctctgctac
     8941 gcccgccagc aggaaataca tcgcacgcag gccgaggatt gcaaacaggt ttgaggtcag
     9001 cacaatgaac gggtcagtgg tcacggcgaa gatagccgga atgctatcca cggcaaaaat
     9061 cacgtcgctc aattccacca gaatcagtac cagcgtcagc ggtgtggcgt acagcaatcc
     9121 attcttacgc acaaagaaat gctcgttgtc gatggtgtcg gtcatgcgca aatgaccgcg
     9181 tagccagcgc accagcggct tgtcgccaat gcctgattca tcttcatggg cgagcgccat
     9241 cttcacgccg gtaaacagca ggaaggcacc gaagatatac aggatccagt cgaactgcga
     9301 aatcagccag ctgcctgtga agatcatgat ggtacgcaga acaatcgccc cgagcacacc
     9361 atacaccagc acgcgacgtt gtaatgccgc cggaacggag aaatagctga acaacatcag
     9421 ccagacaaag acgttatcga ccgccagcga tttctcaatc agataacccg tgagaaaggc
     9481 cagggcctgt ggatcggcaa cggcgcgacc ttcggtttgc accagatacc accagaaagc
     9541 ggcgttaaac agtaacgaca gcgtcaccca gaccagcgac caggccgcag cctgtttcat
     9601 ggtcatggca tgtgccccac gacgcccctg caacaacagg tcgatagcca gcataatggt
     9661 gacaacaaca gcgaatccgc cccatagcaa cggcgtgccg acagtattca tagaagttcc
     9721 ttacacataa aaaaacggcc aacgtcggaa gacgtcagcc gctgcttttt atgcatagac
     9781 ctcgccttcc ggcaaggtct cacttacaac aaaaaaggaa tatgtctgcg tattcctttc
     9841 gggttgcccg gtgaccggat gtggtttttc acacatcgta atgacgatcg accggcaatg
     9901 aagttactcc cctttgcagg taacaaagta tgacagacca ttcagtcagt caatggtctg
     9961 ttacatacct tttacactgt tttactttgg caagtatagt tttacgctat ctgccggaaa
    10021 aatcacccca gtctggcggc ggatctcggt cagcagtttg gctgtgatgc gactgaccgc
    10081 cagccctgga tgatccacca gatgctcatc caccagggta gcgaacagct ctgcttcgta
    10141 gagcatggta ttaatatgct gcggctgggt gagatcctgc atctggctgc cacgcggcac
    10201 aaagcagact ttctggcatt cagacagttt ttcaatcacc agcgctcccg cttcgccctg
    10261 aatctcgctt cccaggacag aatcactgac ctttgagtgc tgcaaggtga cgctgaaatc
    10321 accgtaatcc atcaccacca cgccgtgggc gtcaacgccg cttgccagca aactcgccgt
    10381 tgcctgcacg cttttcggct caccaaataa cgccaccgcc gacgccaggc agtaaaagcc
    10441 gatatccata attgaaccgt tagagaatgc cggattaaag gtgttgggat tctcaccgtc
    10501 caggtaacgt tgataccgcg aggaatactg gcaatagttg aaaaagactt tacgcagttt
    10561 gcctgctttc ggcagcgcct ggcgcaacaa atgaaagttc ggcaggcagg cggttttaaa
    10621 tgcctcaaac agcaccacct gattttcgcg cgcacaggca atggcggcat ccacttccgc
    10681 cagattcgac gccagcggtt tttcgcaaat cacatgaatt ttatggctaa ggaaaagttg
    10741 tgtctgggaa aagtgcaggg aattcgggct ggcaatatac accgcgtcaa tggcatcgct
    10801 ttccgccatc gcttccagcg acgtaaacag atgctcgaca gaaaaatcat tggcgaagtg
    10861 ctgagcctgc tcaaggctgc gggaatatac ggcggttaac ttgtatttac cgctctcatg
    10921 ggcggcctcg acgaactggc gagtgatcca gttcgtacca atcacagcga aacgtatcat
    10981 aacgcgtgga tactccataa agggattctg tcgtcaatgt agcacgcctt tttcatcttc
    11041 gacgtgcgca gatacgcatt attctttcgc cggacagcca tgctcactaa acggcccaat
    11101 catgaccgat ggcccgagat atttcggctg gcgatggagg atttttgcgt caatcaccgc
    11161 gctgacacga gcgagtttct cccgcagttg agtataaaaa ccgtgcttca tattgaggtc
    11221 tggcgcgtta aacccgatag cgtcgatgcc gtactgtttt gccagccaga tggcgcgttg
    11281 gttatgaaat tcttgcgaga taatggtgat atggttttcc ccaaacacct ttttggcacg
    11341 cactaccgaa tccagcgttg agaatccggc atagtcgcaa aagatcactt tcgccggtac
    11401 gcctttagcg atcaaagcct gctgcatacc tgatgcttca tcgtaatttt tatgtccgtt
    11461 atcgccactc accagtagcc atttcacttt tccggcgtgg tacaacgctg ctgcggtatc
    11521 aatacgtcgg gtaaagtagc gattacccgg cctcgccccc agcaataaac ccacgttacg
    11581 cgccgggacg gcgttgacat cgccccaggt cagctgcttg ctggcgttga ccataacgcg
    11641 atcggcgata aaaatggtcg ctcctgccac aagtaacagt aagcaggcaa tctttagagt
    11701 ccggcgagaa aaacaaatac ggagaagaag gcgggcaaat gcgcgcagca tggtcgttat
    11761 ccttaacgaa aggggggagg tcgattttag gcgctaaaga aagaaaagat aacgcctgac
    11821 atcattttca gattttgtcc tgcccaagcg acaactgcgt tagccacagc cgggtgtcga
    11881 actcaagctg gtggtactgc ggctccatat ggcagcacaa ttgatagaac gctttgttat
    11941 gctctttctc cttcaggtgc gccagctcgt gcaccacgat catgcgcaaa aacggttccg
    12001 gcgcgttgcg aaacacggta gcgacgcgga tctctgcttt tgctttcagc ttgccgccct
    12061 gcacacgaga aacagcggta tgtaacccga gcgcgttttt tagcacatgg atcttattgt
    12121 catacatcac tttattgatc ggcggtgcat tacgcagaaa ctgatttttc agatcctgag
    12181 tatattgcca gagagcttta tcggtggcgt agtcgtgtgt tcccggatag cgttttgcca
    12241 gcacatcgcc cagacgctgt tcgttaatca gtgttcgcac ctgcgaaagc agctgctcgg
    12301 gataaccctg aagataagta agattgctca taaccgccca cgaaaaagag aaaaagggta
    12361 tactcacgca cccttttcag gggcaacgtc gaaattttat cattcaggag ggccgatgag
    12421 ccacttagac aacggtttcc gttcactgac actacaacgt tttccggcga cggatgacgt
    12481 taacccgcta caggcgtggg aagcggcaga tgaatatttg ctgcaacagt tggacgacac
    12541 agaaatcccc ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa gctgcgcgct
    12601 ggcggaacat aagccgtaca gcattggcga ctcatacatc agtgaactgg cgacgcgcga
    12661 gaatttacgc ctaaacggga ttgatgagtc gagcgtgaag tttctcgaca gcaccgccga
    12721 ctacccgcaa cagccgggtg tggtgctgat caaagtgccg aaaacactgg cattgctgga
    12781 acagcaactg cgtgcgctgc gcaaagtggt cacgtcggat acacgtatta ttgccggtgc
    12841 caaagcccgt gacattcaca cttccacgct ggaactgttc gaaaaagtgc tcggcccgac
    12901 caccaccacg ctggcatgga aaaaagcgcg cctgattaat tgcacgttca atgaaccgcc
    12961 gctggccgat gcgccgcaga ccgttagctg gaagctggaa ggtactgact ggactatcca
    13021 taaccatgcg aatgtcttct cccgcaccgg gcttgatatc ggcgcgcgct tctttatgca
    13081 acatctgcca gagaatctcg aaggggagat tgtcgatctc ggttgcggta atggcgttat
    13141 tggtctgacg ctgcttgata aaaacccgca ggcgaaagtg gtgtttgtcg atgaatcgcc
    13201 gatggcggtt gcttccagcc gtttgaacgt tgaaaccaac atgccagagg cgttggatcg
    13261 cagcgagttt atgatcaaca acgcgctctc cggcgtggag cctttccgct ttaatgctgt
    13321 gctctgcaac cctccgtttc accaacaaca tgcgctgacc gataacgtcg cctgggagat
    13381 gttccaccac gcccgccgct gcctgaaaat caacggcgag ctgtatatcg ttgccaaccg
    13441 ccatctggat tacttccata aactgaagaa aattttcggc aactgcacca ctattgcgac
    13501 gaataataaa tttgtggtgc tgaaagcggt gaagctggga cgtcgtcggt aagattggga
    13561 gttacctgcc ggatgcggcg taaacgcctt atccggccta cgttcagcac gttaccccca
    13621 ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc tgctcagttc tggaatcgcg
    13681 cttccataaa acaaaaaatc cctgtcacca tcctctacat tctcttgttt agcgtttttc
    13741 tacgtttatt cttccgtcac acagataaat tcatccgttg ccaatctgtc aacggatgtt
    13801 atcatattgc caatttgtca acggacgtga tgaagaatgc acctgataac tcaaaaagca
    13861 ttgaaagatg ctgcggaaaa atacccgcaa cataaaacgg agttggtggc tctggggaac
    13921 acgattgcta agggatattt caaaaaacct gagtcattaa aagcagtatt cccatctctg
    13981 gataacttca aatatctgga taagcattat gttttcaatg ttgggggcaa tgaattacgt
    14041 gttgtagcaa tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt tatgacgcat
    14101 aaagaatacg atttctttac cgctgttcat cgtactaagg ggaaaaaatg attgcgattg
    14161 ccgacatctt gcaagcagga gaaaagctaa ctgctgtggc accttttctg gcgggtattc
    14221 agaacgagga acaatacacc caggcgctgg aactggtaga tcatctgctg ctcaacgatc
    14281 ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa gaatcagcgc
    14341 ccgaatttgc ggaatttaat gccatggctc aagccatgcc tggcggtata gccgtgattc
    14401 gtacccttat ggatcaatat ggtttaaccc tttccgatct gccggaaatt ggcagtaaat
    14461 ctatggtgtc acgcgttttg agcgggaaga ggaaattaac gctggaacac gctaaaaaat
    14521 tggcaacgcg attcggcatt tctcccgcct tgtttattga ttaacgcgac gtgccggatg
    14581 cgttgcatcc ggcgcttcaa ataacgattt aaatctccag cgccagccgt gttccctggg
    14641 caattgcccg tcgtgcgtcc agctccatag ccacatcgca gccgccaatt aaatgcacag
    14701 ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc cctgcgcaga
    14761 tcaccacatt gtccactgct aatacctgcg tttcgccgtt gatcaccaca tgcagcccgt
    14821 catcgtcaat cttctgataa cttacgcctg ggatcatttt cacaccccgc gagagcaggg
    14881 tggtgcgatg gatccagccg gtggttttgc ctaacccctg tcccggtttg ctggctttgc
    14941 gctggagcat cacaatttgc cgtgggctac gcgggatctg cattccctgc gggcttaagc
    15001 caccagcctg ttgtaggctg ctgtcgatcc cccactcatt acagaacctg gcgatattct
    15061 ggctggtgga ttcgcccggc tgacttaaat acatcgccgt atcaaaacca atcccgccac
    15121 aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca tcgagataac
    15181 tcaatacctt cggatgatcg atcccgtcga taggcggaac gcgcggcacg atcccactgg
    15241 cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt cacggtgtga ttgagtttca
    15301 gcgtcacgcc cgtcacttcg atcatccggc ggtagtagcg cagcgtttcg taaaactcct
    15361 ctttgccggg gatctgtttg gcgatattaa actgcccgcc gatctcgctg tgcgcatcaa
    15421 acaatgttac ctgatgccca cgcgccgccg cgttaatggc aaacgccagc ccagcaggtc
    15481 cagcgccgac caccgccaga ttttttttct gcacggcggg aaggattggc attttggttt
    15541 cgtggcaggc gcgaggattc accaggcacg aggtgacttt gccaacgaag atctgatcga
    15601 ggcaggcctg gttgcagcca atacaagtgt tgatctcatc ggctcgccct gattgcgctt
    15661 ttgacagcag ttccgcatcc gcgagaaacg gtcgcgccat cgatactata tcggcatcgc
    15721 cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat ccggttggtg gttaccagcg
    15781 gcagcgagac gtggcctttc agtttgcgcg tgacccagct aaatgcgccg cgcggcacgg
    15841 gcgtggcgat ggtcggaatc cgcgcttcgt gccagcctat tccggtgttg ataatggtcg
    15901 cgcccgccgc ttcaatggcc tgcgccagtt ctaccgtttc ggcaaaagtg ccgccgcctt
    15961 cgaccaggtc gagcatcgac aaccggtaaa taataatgaa gtcgttgccg acgcgttcgc
    16021 gcaccgtacg cactacttct acggcaaatc gcatccggtt gcggtaatcg ccgccccact
    16081 ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac ccttcagagc
    16141 ccatcacttc gacgccatcg tatccggcct cccgcgccag ttgcgcgcag cgggcgaaat
    16201 cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa cggttgatgg
    16261 gggcctgcaa tgcggacggt gcgaccagat gcggttggta gctgtagcgc ccggtatgca
    16321 aaatttgcag ggtgattttg ccgccttcct gatgtacggc ttcggtaatg gtgcgatggt
    16381 gtgggatctg gctggcgtcg ttgagcattg cgccaccttc catgccaacg cctgttaaat
    16441 ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt tcggcataaa
    16501 acgcagccag ccgttcagca ccgtccgggt attcctccag cccggtgtgc attgaaccca
    16561 tcaacacgcg gttttttaac gtggtaaaac ctaaatccag cggggcgaac agcgacgggt
    16621 agctcataaa attgtccagt atgtaaaata attgttatgt ggtcggatga gttctaattt
    16681 agccgtcgat ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga ttcaaaaaga
    16741 atacgcccgg tggtaacgcc ggatgcgacg cttgccgtgt tttatccggc ttgggggatt
    16801 gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agccgttgca aattactgct
    16861 tacggaaaaa atcgttatac aacatataca aatgagcggc actccaggag aaatttggtg
    16921 cgccttgctg cgcgcctgtc agcgggttgt aattctcctg aattgggcca tcggcggtta
    16981 atcctttggc gtgcctgaag aacgtatccg ccagtttcag ggcatcatca cgataaccgt
    17041 aacgctccat ccctttcaga ccaaaccaga actgatccac ccatacgcgc ccgcgccagt
    17101 agatatcagc gccaaaagcc ggattggtta acgccgccgt tcccagcgga acaaaggtgt
    17161 tgaactcttt aggatcgagc atcaccttca ccaccgcgtc ggcattggcc tgcgttgccg
    17221 caccgttaaa cagcggcgac cagccttccg gccctttacc gcgctcaaca atcggtttgc
    17281 ccgcgcagcc gttcgccagt ggtttatctt caatacgtac gtcatagtag aactgggtag
    17341 tcgggtcgaa catacaggtg ttgatgtagt ccgcgagctg ctgtgccaac tggcgatagc
    17401 gtttggcctc ttctggctta ccgaggatgg ttgccatctc cgccagataa tggttatcgc
    17461 tgtacatata gttggcctga tccaccgact cctgcaatag cgagtagccc agcaacgttc
    17521 cgtcctgact gcggttttcg gcgaatttca ccgtccagtc gctacgtttg ccgccattag
    17581 cgacatattt atccaactgt tctttgtcga taaaaccaaa gacggcggca tcatcacgcc
    17641 ccgattccca cgacgcagcg acctgtgccg gaatttccag actgtcgtac tggcctttct
    17701 ccaccacgcg ggcgtagttg ttcagcccag actgcgtctc ttctttatcg ccttttttca
    17761 ccgtaaacag catctcgccg ctctcggtgt tgtgggcttt gtcgcgggtc gcgccatatt
    17821 ccggcacccc gttgccgtta tgatcgcggt tacgtaacca ccagtcgtga taggccacca
    17881 gtttcgggta catctctgcc aaccaggctt tatcctgggt gacgttgtat acttccatca
    17941 ccgaccaggc ggcaaggctg ggtttggtat tacgttcgtt ccagttgccg ccatcgccac
    18001 cacgctcggg gctaaggttc cacgctatca ggtcggggac aaagcccaca tcctgcggac
    18061 gcacgctgtc gccaggctgg atctgccagg agaagaccgc gcggatattc tctttggcga
    18121 tatccggatt gaaatgcacc atcgcaaacg cctgcttcca ggtatcccac ggccaggtct
    18181 gattgccgga gaaccagcgt ccggtcaccg atggcgtgac ggtgttataa ttcaccgcac
    18241 cgcccggtga gcgccagtta ccgttgagcg tttcgatcgc tttcaccgcg acgcgcgtct
    18301 gttccggcgt cgcatccgga ttggttaacc ctttcttcag gtattcttcc cagcgttgct
    18361 gcgaggcggt gagataaaac gccggacgcg ccagaatatc gcggatctgc atttgctctt
    18421 tgctaacttc ctgagcggtc agcagatggg aataggtggt gtagagcgtg gtcgaaccgt
    18481 tgatatgcgc cttactggta aagcgattac cgttgatttc agtctgcacc ggcagggatt
    18541 tatgcacctg atattccgat tcgccggagg tcagcagatc ccaggtggcg cgcactttgc
    18601 caaaggtgac tttcaggcca tcacgggtgg cgctgatttt gcgctgatag tcagggtatt
    18661 cgccagcaat ggttttatcg gaaagcggtt tcccttcttt cgcttccagt ttttccagca
    18721 gttcgccatc ccacaccaga tccagcggtt tattgctgat gattttagtt tccagtagtg
    18781 acgtgcgcgg cgtggcgaag cgcagagtca tttcgacctg cacatctttt gctgtcagtt
    18841 tttgcaccag cgcgccggga atactgtatg cctccagcgt gaagttgact ttcttgccgt
    18901 cttgccagac agtcaggcgg tcgaaattgc tggccataaa gttgatgtat tcttccgtca
    18961 gcagcgcaac gcccggaaag ccgcccatgg tgttcgggcc gtctggcaac agatgaccat
    19021 gccaggctcc gagatcgaaa aacggattaa aacgctggtg atcgtcgtaa tcgtaatcct
    19081 tcatgtactg cggcgcacca gtacggttaa tcacgttttt ataattgtcg gcgttagcgg
    19141 catgagcgga aaaacttatc agcagagcgc aggttactgg cgttaaaata gttttgattt
    19201 tcatgaaatt acagtcctta ttaccagtag aaatattgca tcaggaagat ttggtcgctg
    19261 ggatcgttag aggcattttt catgtagaag cggctatcaa aagccgtggc gaaacgtccg
    19321 ccgccatatt cgtaccagat accaaactgc atgaaagagg tggtttcatc gtcagcgtgg
    19381 tcagggcgat gtttggcgta ggtgtaagcg gtggagagat aaacgccctt taacgcttca
    19441 tacatggcgc tcaccatcat cccgctttca cttcccggta cgtccagccc gtcggcacgt
    19501 ccggtatggt gccaggcgcg agcggcaaag ttaggggtaa acaggcccgt gagatagagt
    19561 tgtcctgtgc cgtcggtaat ttccagacca ttcatccagg tgatgccttc ggcaaggtca
    19621 tactggatgt aaccgttcat ccattgccgg ataggtgtat ttgtcgtcgt agcgatcgta
    19681 cttgccaaaa tgtagccagg ctttgctttc gtcatgatgt ccggcaggtg tggcggtgac
    19741 gctataacga aagttgccgc ttaagttctg taccttcagc gcggtcataa tgtcgcgggt
    19801 attgggaatg acgtagccca aatcgggtgt gaagtcgccc caccattgca ggtcatccag
    19861 cgaggaatcc tgacgcagac tgagcatgat ttcggtgcca tcattggtac gataaccgcc
    19921 atagaaacgg ttaatcccac cttcgaaacc accccagcta tggtcaggca cccaggcatt
    19981 accgtcgtgg tcggcctgca cggtccagcc ttcgccgtaa atcaggccga accaggagcc
    20041 gtacttcatc tccaggccac cttcaatata ggtgccgtcg ctgtatttat gtttattctc
    20101 accctctaaa tccatatatc caccaagccc taattcgccg taaaaacgaa atgaaggaga
    20161 agatgtcgag gtaattgcgg gtgtttttgt gtcgtcatgt tctgccggac gtgcttcttc
    20221 tcctgccgca gaaaaggcgt agcaaaacgg cagagcaagt aatgctctgc atggtgcagt
    20281 gattaatttc attgtgttaa tccatgagct aaagaaaaag tacagccttt tatcgttgta
    20341 attataaaaa tataaaggca gaaactattt tccctctcct catccaggaa gagggaaaat
    20401 gcctgctcat tttttattag tctgcttaat caacattgct ggctggcgtc gcttcaatat
    20461 ggcgctcttc agccgttaat ccctttatat atttactgta tttccaccac gcaaagccga
    20521 ggaagataac aataccgccg acgttataaa aaatgatggt cagaatattc gcgccagtcg
    20581 ggaatgtcga agcgacaaat cctacggcaa atatcgcaat cagcattgaa acaacgataa
    20641 taccggtgcg gcgggagccc atacggaaat cgcgtggcag gtgatctaat ttggcgcgta
    20701 aattcaggta agccagcatg atgaataacg gcggaagcat ggacgctgcg gcggtcatat
    20761 taataatggt attcatcaga tcctgcacgg tattggaacc gagcatcggg ataatcatca
    20821 gcgggatgac gatcagaaac tggatccatg ctgcgcgcgc cggaacgccg ttttcgttaa
    20881 gttcgacggt tttcttacca aagatgcctt ccgggatttc ggagaagaaa attttcaccg
    20941 gcgttgcggt ccacatcagc agggaaccga acattgcggt aaaggacacc agaccgacaa
    21001 agcgattcat caacgcttcc ggcagaccaa aatacgccgc catgccgtgg aatacctgca
    21061 ccgatccgcc ggtaaatttc aactctttgc tgctgacgaa gacgttaatc agcaccgagg
    21121 agacggaata cagtacgccg ataaaaatcc cggcgaggat gatcacttta acgaacgatt
    21181 tcgaaccgcc tttgacgtcg ttaacgtaca ccgcgacgga ctccgcgcca cctgcggcca
    21241 taaagatcca ggtggtaacg ccgaggaacg cccagttgaa gttcgggatc atcgcgtcaa
    21301 cggtgatcgg atcggcaggc tgtacgccgc caaccagtgc cgtacctgcc agtaaaatgt
    21361 aggagagcgt taacagcagc atcagcgttg aagtgacgga ggtaattggc cccagcattt
    21421 tcgccccgtt ggtggaaacc caggtggaga aggcgaacag caccatactg ataacggtgg
    21481 tggcaaccgg cgtcataata tattcgtagc cgaggaaggc atacgaagca taagcaataa
    21541 cgcgcggcaa cagcgaggtg aaaaagaaca gatttacgaa ccagtaagtg taggcagtaa
    21601 taaatgccca acgtccgccc agcgaacttt ttacccacgc gtagacaccg gcttctgagt
    21661 ttttatttaa cgaaacaaat tctgcgatga tcagacagaa gggaataaaa taaaaaatcg
    21721 tcgcgaggaa aaacatcggt gccgaggcca gtccaagctc aatattatta ttgataacgt
    21781 tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa gccgaatttg ccgattgtat
    21841 tacgtttggt atcagacata acacatctcc gaaaatgtgt agggtagcgg tggcggcaaa
    21901 ctccttttgc cgccgtagtt gttatttgtt atggaaataa ccatcttcga tggtgacttt
    21961 gagaaccact tttttgaccg cgttattgca gataaaccga tacgcttcat ggatatcgca
    22021 aataacgatt tgcccttcgt ggacctctac ggtttctccg cagcctttta aatattcacg
    22081 gtcagtttca tcacgataat attccactac ctgtaatgtc tctttcggcg catattcaat
    22141 tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag cgtcgatgcc cggtaaatag
    22201 cgcatcggtc gcggagtcat tttccacacg gtaggtcaat gaatcaccga tggagtgggc
    22261 gacgccaggc tgaatgttgt cgatattttc aatcgcttca acgcagcgtt gccacttctt
    22321 gccagaggcg taaatctggc ggaactgttc taagttatcg atgatcctca ttgcttattc
    22381 tcgctgtgca aattcgtgga aaagaaccct gcgccgaact catacgacgc caggctttgc
    22441 gcggtagctt ctccgccaga aaccggcagc aacgtaaagc cgtagctgaa gtcacggaac
    22501 cagacgcgcc aggagtccag cacctcgctg ccccaggagt tggagccgag gccaagtagc
    22561 tggtggtcga ggttcagggt gatgtcatca ctgcgctgca gctcgttaca gtgctgggca
    22621 gcgtggatgt tttcctgggt atagtgccag gcgctgaagt taattgggcg ctgcggaacc
    22681 accagcagac cgttgccgtg gcggttagtc agtgccgtcc agcggacatg ctgacggttg
    22741 ccgttgttct gcgggaaggg atagttctcg aacatggcat cgacggtgct gcgccagata
    22801 tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc ccggtccacg accgtaatac
    22861 gccacctgat cgtattcgcc gttaatcccc atggtgaaac cgatgcacgg aatgatgtgc
    22921 ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa cctggccatc ggcagcgatg
    22981 cgccagatgt aggtacagcg catcccgaag tcaaacaccg gcggtgcaat aactgtgcgg
    23041 ctgatgatca acacttcacc atcgctctgt tctacggcaa agtcgcgcag atgttcctgc
    23101 atgatctgca aatgattcgg ctgccacaac ccttcgtact cctgcttgtg gttgtcgatc
    23161 atcggcttga agaagttgat ctttggctcg cgggtcagca gcgactcgcc atttacctgc
    23221 caggatgtcg gtttgccact catttttgag aaggtgatcg cgaagttgta gccgcgaacg
    23281 gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg cattatttgg tgcgaaaggc
    23341 actggctgcg cggtgttttc cttcagcggg aactgataag tggcgataga atgcccggct
    23401 tcgctgtagc gggtgtggga atctttggtc accgtaatgt tgaggaacgc ttcgcgggcg
    23461 tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt tcggcgcaac gtcgcgcagt
    23521 ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca cctctgcgtg cagggtgtag
    23581 tcatcaagcg tggtaaacca cagtttattt tcgactttca actcgccgcg agtcagatcc
    23641 cgcgcgtgga tttttaccgg cgcgataacc tgtttgtact ctttcaggcc cgatcccggc
    23701 gtctgatcgg aatagatcaa accatcaaga cagaagttat agttgttggg atagtcgccg
    23761 tagtcgccgc cgaatttata ccagacattg ccgtggtcgt cctgtgcctg gatcccgtgg
    23821 tcgcaccact cccagacata atgaccctga atgcaatcgt gcttatagaa gacgttctgg
    23881 tactccgtca gcccgcccgg tccgttcccc atcgcatgag catattcaca gatgatgcgc
    23941 ggcttcggat gcgggtattc accaaactca ttcatcagcg gcacgcgggt gtacatggtg
    24001 gaaataatat cgaccacttc agcatcgcga tcttcttcgt aatgcaccag tcgcgtgtca
    24061 tccagcgctt tcgccgcatg gtacatcgcg cggatgttac agccatagcc ggattcattg
    24121 cccagcgacc agatgatgat cgacggatgg tttttctgtg cgtggatatg gcgaacaatg
    24181 cgctcgacgt agactttttc ccactgcgga tcgtcggtaa tacggctgat atcgccgaca
    24241 ttagcaaagc cgtgcgattc gacgtcggtt tccgccatca caaacaggcc gtagatatca
    24301 cacagttcgt aaaaacgcgg atcgttcggg tagtgagcgg tacgcacgga gttgatattg
    24361 tgctgcttca tcaactggag atctttctcg acgcgatcca ttccaacggc gcggcctttg
    24421 cgatgatcgt tgtcgtgacg gttgacgccg tgcagcatca cataacggtt attgatccag
    24481 aacagaccgt cgcgcacttt gatatcacgg aagccaacgc gttgtggcac cacttccaga
    24541 acgttgccgt tggcgtcttt cagcgtcatg accagatggt aaagataagg ggattctgct
    24601 gaccattgct gcggctgttc gacagtaaaa gcaaagctgg cgctggtcag tttttcaatt
    24661 gccaaatgat caatggcgct gctgtgcacc acgcgttcgc catcaaacag ggtatattcc
    24721 agcgtcgtga cgacagggga ggcggcgaga ttttccagca ccacttcgca ggaaagcgtg
    24781 gcatcgcaat aggcttcgtc aaagtcggta cgcacagtga aatcgttaat atgcgttagg
    24841 tgttttccga ccagataaac atcgcggaag atccccgccg accaccacat atcctggtct
    24901 tccacgtagg tagagtccgc ccactgcatc acgcgcacac acaacaggtt gtcgccggtt
    24961 ttaaccatcg cgctgatgtc aaactctgcg gtcaggcgac tgcccttgct gaaacccaca
    25021 tactgaccgt taacgtagac ttcaaaatag gtttcgacgc cgtcaaattt aatcagcgtc
    25081 tgtttaccct gccagccgtc gctgagggtg aaaatacgtt gataggcacc ggttgggtta
    25141 tcgctgggga caaacggcac atcgatgggg aacggaaaac cttcgtcggt atattgcagt
    25201 ttgccgtgac cttccatttg ccacatggcg gggacggtaa tatgccccca gtcagccatt
    25261 aactcagagg tgaaggcttc tggtacttgc agcggatggt caaaaaagtg gaaattccac
    25321 tgaccgctta agggcagaaa caggctgctg gtttcgcggg caaaggtacg cgcttgcgca
    25381 acagaatcat atgaaaaaaa gtacgcacgc ggcgcaagtc ggttttcgtg ggtgagctga
    25441 atgttttccc agcgattcat agggcctccc ggtagagaga aatcggaacc attcatggtg
    25501 atggtagttt gtgtaattgc tggggagttt agtaaaattt ttactaaaaa gtagcaggta
    25561 aatgcattta ctttaactgg atcacgaaaa taaacggcag cactgtccgg caaaaatgat
    25621 tgccggacag tgtgtaaggg gatttaacgg gtcgtgccgc gcagttttaa tttgctggga
    25681 acgaagacta gcagcggcag cgcgcgacca tcgcgggctt tttcatacac caggttaacg
    25741 ccctgacttc ccatcatctc ggaatggatg cgcacggtgg agagcggcgg aaaggtaaat
    25801 cgcgcggtgg ggatatcgtt aacgctgata agcgaaatat cctgtgggat gttcaggcca
    25861 cgttcatgaa ttgcccgcag tacgccgata gcaatggaat cggaagcaac aaacagtgcc
    25921 ttcggatagt cttcccgcgc cagcatttgt tttgccagtt cataacccga cgaactggaa
    25981 aaaccgccgc gccagatatc ctcttcgcgt accacttgtt tcagtcggcc atattccgca
    26041 aaggcgacct cacgaatatc cgccttgcca ggctcatctt caccgccaat aaaaccaata
    26101 cgattaacgc cctggttgat atagaagtcg atgatttctt tactgatgcg tgccagatcg
    26161 atatccaccg catcgtaacc gctgccgggt tcgtgaaagt cgataaaaca gatattgtcg
    26221 gtcaacgcgc tggcagcggc gcgcagggcg ggcgtgggtt tgccgacaat taaaataccg
    26281 gtgacgtttt taatgtctgg taatccgttg tgttcataac agttggtcag ttcgatcgcc
    26341 agcttttcgc actgagtttc aatgccgtgg cggatcgcca gatagtaagg atcgttgatc
    26401 tccagctcct gctggtagct gtagatagcc agaatatggt gttggttgac tgcgcctgtc
    26461 tgtagtttac gggcgctact ggtcttgtac tccagctttt cggcgatctc gagaatgcga
    26521 tgtttcgtct cttctttcac attcaatgtc ggatcgtcat ttaagaccct ggatactgtc
    26581 gccagggata cgccagcttc gattgcgatg tcttttaatg ttgccatgtt tacctttttt
    26641 actttccctg atgtgcgccc ccattgtaga ggaagtgctt atcgggtcac ctgttttcag
    26701 taaataaagc gcgtttcttt gacgctgtaa cggtaacaca gcggcttagg aatgtgatca
    26761 cgcctgcatt ctccattttt agcctacagg attgagaaaa acttttgcta tagtttagta
    26821 aaattttacc cgaagggagg aagcaatgga aaccgtggct tacgctgatt ttgcacgtct
    26881 ggaaatgcgc gtcggaaaga ttgtggaagt gaaacgccat gaaaacgccg acaagctgta
    26941 catcgtacag gttgatgtgg ggaaaaaaca ctgcaaaccg tgaccagcct ggtgccgtac
    27001 tacagcgaag aggagctgat ggggaaaacg gtggtggtct tatgcaatct gcaaaaagcg
    27061 aaaatgcgtg gtgaaacgtc ggaatgcatg ctgttgtgtg cggaaaccga tgacggcagt
    27121 gaaagcgtgt tgttaacgcc ggagcggatg atgcctgcgg gcgtacgcgt cgtataaaaa
    27181 gatcggatgg cgacgtcgta tcgccatccg atttgatatt acgcttcttc gacacttact
    27241 cgcatggccg ccagcgcctt acgcgccgct ttgatcacca gttcacactg ttcaatggtc
    27301 agtgtcagtg gcggttcaat gcggatcgtt ttggcgttat tgagcgttcc ggccaccagt
    27361 acgcgctggc ggaacatctc gctggcaaag ttatagccga tttcgttatc aacaaactca
    27421 atcgccatca acatcccttt accacgcgct tcctgtacca gatcgggata ttcccgcgcc
    27481 agttgacgga aaccatccag caacatatcg cctttttgct cagcctgagc cggtaagttc
    27541 tgctccagca acacattgat ggtcgccagc gccgccgcac aggccagcgg gttgccgcca
    27601 aaggtggtgg tatgcaggaa tgggttgtcg aacagaactg aaaacacctc ttcagtggcg
    27661 atggtcgcgc caatcggcat cacgccgccg ccgagggctt ttgccagaca cagaatgtcc
    27721 ggctgaacgt tctcatgttc gcaggcaaac atcttgcccg tgcgccccat gcccgtttgt
    27781 acttcatcga ggatcatcag tgcgccgaac tcatcgcaca gcttacgcac ggcggtgaga
    27841 tagcccggcg gcggcagaat tacgccacct tcgccctgaa tcggttcgag gatcaccgca
    27901 gccacatcat caccggtttt tttgcactca ttaagagccg tgcgcatggc ttcgatattg
    27961 ccaaacggca catgacggaa ccctggcagc agtggcataa acggtttgcg gaaggtcgat
    28021 ttcgccgtgg ccgacagcgc gccaagtgat ttaccgtgga acgcgccgct ggtggcaata
    28081 aaagtaaatt tgccgcgcgg cgactgataa gccttcgcca gcttcagcgc cgcttcaacg
    28141 gactcggtgc cgctattaca gaagaagctg tatttcagtt taccgggcgt tagcgcagca
    28201 agggttttcg ccagcatagc ccgtaacgga tcgagtagct cctggctgtg cagtggttgt
    28261 ttcgcaagtt gattctgtac ggcggaaacc acaactggat tacggtgccc cacgttgaaa
    28321 attccaaaac ctcccaggca gtcgataaac tcctgtccct gggtgtcgac aagcgtattt
    28381 aaacctcccg cttgccactc tacggctccg taatccccgc cggcggtaac agatttgcga
    28441 tactctaaaa accccggatt gacatgctct ttgaagtatt caatcacctc tcggttaagt
    28501 gctttcatct cctcatgatc cagcgttcgc ttctcaatga gattcagggc gtgggcgctg
    28561 cacgctaaag ccgatgcgct cgaaggtaac ctgttcaaaa tatgctccgg aggctcgcgt
    28621 atcacatgat accgatttaa gtattgcagg gattgcgcca ctccggctgc gatcgcgaaa
    28681 atcgggataa acacaaatgc ggttatcatt tgtgcaatat ttaatgctat ccgttaacat
    28741 ttgttgttat tttgcacggt ttttgtcggg tatggatttg atgattgctg cgcaaatgca
    28801 cgttttcagg gcaataattg cctgattttt gggcaagata attaacgttt tcgtatagta
    28861 acagagtgaa agtattttaa atcaaagaat tagatttgtg ctttaatcgc aaattgcgat
    28921 ctaaatcaaa ttaatcggtt aaagataacc gcagcggggc cgacataaac tctgacaaga
    28981 agttaacaac catataacct gcacaggacg cgaacatgtc ttctcatccg tatgtcaccc
    29041 agcaaaatac cccgctggcg gacgatacca ctctgatgtc cactaccgat ctgcaaagct
    29101 atatcactca tgctaatgac acttttgtgc aggtgagcag ctttaccttg caagagttac
    29161 aagggcagcc gcacaacatg gtgcgtcacc cagatatgcc aaaagcggcg tttgcggata
    29221 tgtggttcac cctgaaaaaa ggggagccct ggagcggcat cgtgaaaaat cgccgcaaaa
    29281 atggcgacca ttattgggtg cgggccaatg cggtaccgat ggtgcgcgag ggaaaaatca
    29341 gtggctatat gtcgattcgt acccgggcga cggatgaaga gattgcggcg gtggagccgc
    29401 tgtacaaagc gttgaactcc ggacgtacca gtaagcgtat tcataaaggc ctggtggtgc
    29461 gtaaaggctg gctgggtaaa ctgccttcat taccgcttcg ctggcggacg cgtggagtga
    29521 tgaccctgat gtttatcttg ctggcggcca tgctttggtt tgttgctgcc ccggtggtga
    29581 cgtatttcct ctgtgtgtta gtggtattgt tggcaagcgc ttgttttgaa tggcagattg
    29641 tgcgcccgat agaaaatgtc gcccgtcagg cactgaaggt ggcgaccgga gagcgtaata
    29701 gtgttgagca cctgaatcgc agcgatgagc tggggctgac attacgcgcg gtagggcagc
    29761 ttggcctgat gtgccgttgg ctaattaacg atgtctcaag ccaggtgtcc agtgtcagaa
    29821 atggcagtga gacgctggcg aaaggcaccg atgaactgaa cgaacatacc cagcagacag
    29881 ttgataacgt tcagcaaacg gtggcgacca tgaaccaaat ggcggcgtcg gtgaaacaga
    29941 actctgccac ggcgtcggct gccgataaac tttctatcac cgccagtaat gcggcagtgc
    30001 agggtgggga ggcgatgacc acggtgatca agacaatgga cgatatcgcc gacagtaccc
    30061 agcgcattgg caccattact tcgctgatta acgatattgc gtttcagacc aatattctgg
    30121 ccctgaatgc ggcggtggaa gcggcgcgtg ccggcgaaca gggcaaaggt tttgcagtgg
    30181 tggcagggga agtgcgtcat ttagccagcc gcagcgctaa tgctgccaac gatattcgca
    30241 agctgattga tgccagtgct gataaggtgc aattcggttc gcagcaggta cacgccgccg
    30301 gacggacgat ggaagatatt gtggcacagg tgaaaaacgt cacccagttg atcgcccaga
    30361 ttagccattc aacgctggaa caggccgatg ggctttccag cctgacccgt gcagtggatg
    30421 agcttaacct gatcacccag aaaaatgccg agctggtgga agagagtgcg caggtgtcgg
    30481 cgatggtgaa acaccgcgcc agccgactgg aagacgcggt gacggtgctg cattaatcgg
    30541 tatgccggat ccggcggttg gagcacaatg cctgatgcgg cgattaatcc agttgcgtaa
    30601 tatcaaaagc ggcgcggtcg atcaccgcaa tgatcttttt tatttgtgca tcactgatat
    30661 cgctctggtt gacgcgtaaa tccagcaccg ctttaaaatt atccagcgcc cgcttcattt
    30721 gcgggttctg gcgcagcgcc gcgccaacgc aacgcgcttt gatgcgttct tcaatcatct
    30781 ccacctgttc gcggttttct tccagccact gcgcgccttg ttcggtcagc gcaatctgct
    30841 tcttaccacc ttcctcttcg cggatggtaa tcagcgactg ctcctgcaga aaatccagcg
    30901 tcgggtagat gacgcccggg cttggggtgt aattcccctg ggttagattc tcaatcgctt
    30961 taatcaattc gtaaccgtgg ctgtcatcgc gcgagagaat atccagaatc accagacgca
    31021 attcaccgtg accaaagaaa cgttgccgac gaccgccgcc gcgaccgtgg cgaccaccgc
    31081 aacattgacc gtgttcatgc tggtgaccgt gtccgcagtg ctcgtgttct gacttctcac
    31141 ctttgcagca gccctcatgg cgtggctggc cttcatgttt acaacaccct tcgtgatgat
    31201 ggctcatatc agcctccttt tgcttgatta gatatatcta atttatatct gatgtgagca
    31261 ttactgcaag tacaaaaatg atttttaaac gtggaagcac ctcacatcta ggccggataa
    31321 gacgttcacg ccgcatccgg cagcattgct atggcactga atgccttcac atacttcaga
    31381 catatcagtt ctacaaatcg cttgcattta tcatgattaa caatcattat catttgcgaa
    31441 ttttatttag atatatctga ttaccatcac gaaggcgata acaatgaata acaccccccg
    31501 ctacccgcag cgcgttcgca atgatctgcg cttccgtgaa ctgactgtgc tacgcgttga
    31561 gcgcatcagc gccggttttc agcgcattgt ccttggcggc gaggcgttgg acggttttac
    31621 atcgcgcggc tttgacgatc acagcaaact cttctttcct caacctgacg ctcactttgt
    31681 gccgccaacg gtaacggaag agggcatcgt ctggccggaa ggaccacgcc caccgtcgcg
    31741 tgactatacg ccactgtatg acgaactacg ccatgaactg gcgatcgatt tctttattca
    31801 cgacggtggg gtcgccagcg gctgggcgat gcaggcgcaa ccgggcgata aacttacggt
    31861 ggcaggtccg cgcggttcgc tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg
    31921 cgatgaatcc ggaatgcccg cactgcgccg ccgcctggaa acgttgagca aacttgccgt
    31981 taaaccgcaa gttagtgcgc tggttagcgt gcgggataac gcctgtcagg attatctcgc
    32041 gcaccttgat ggttttaata tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg
    32101 tctggcgcag atgcaaatcc ctgccgatga ttacttcatc tggataaccg gcgaaggtaa
    32161 agtcgttaag aatttaagcc gccgctttga agcggaaaag tatgacccac agcgggtacg
    32221 tgcagcggct tactggcacg caaagtaact gatatataaa aagaaaaagg ctgacgattt
    32281 ctcgtcagcc tttgctatat ctggtggccc ctgctggact tgaaccagcg accaagcgat
    32341 tatgagtcgc ctgctctaac cactgagcta aggggccatg gtagcggatt ataaagtaac
    32401 tccgtgtcgc aatccagcca ttaccgcgcg cctgctgttt ttataaacaa tgtattttca
    32461 atccgttata cttttcctgg tgatgttaga aaggagtaaa tatggttgag gatattttgg
    32521 ctccagggtt acgggtcgtg ttttgcggta tcaaccctgg gctttcatcc gccgggactg
    32581 gttttccctt tgctcatccg gcaaatcgct tctggaaggt gatatatcag gccgggttta
    32641 ccgaccgtca gttgaagccg caggaggcac agcatctgct ggattatcgt tgtggcgtca
    32701 ccaaactggt agaccgtcca acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg
    32761 caggtgggcg taagctgatt gaaaaaattg aagattatca gccgcaggcg ttggcgattc
    32821 tgggcaaaca agcatatgaa cagggattca gccagcgcgg tgcacagtgg gggaaacaaa
    32881 cgctcaccat tggttcgacg cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg
    32941 tttcactgga gaaactggtt gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg
    33001 ggcgataaaa aacgccaccg aa
//
LOCUS       DBKEKI010000036        31548 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.036, whole genome shotgun sequence.
ACCESSION   DBKEKI010000036 DBKEKI010000000
VERSION     DBKEKI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.036"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..2102)
                     /locus_tag="ACUQPX_001635"
     CDS             complement(<1..2102)
                     /locus_tag="ACUQPX_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HHV9056377.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKSENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLEPLEPLEPLEPLEPLEPLEPLEPLA
                     TLAWTLGFEYHHGLLEKMWKEILKNHAHDSIGCCCSDKVHREIVARFELAEDMADNLL
                     RFYMRKIADNMPQSDADKLVLFNLMPWPREEVINTTVRLRASQFNLRDDRGQPVPYFI
                     RHAREIDPGLIDRQIVHYGNYDPFMEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKS
                     DAEGILENAFWQIALNEDGTLQLVDKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWV
                     ITSANAKPQCEIIHEAWQSRAVIRYEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNR
                     RIDVDINLDNQADDHRLRVLIPTSFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKE
                     APVPVWNMLNYAALQEGR"
     gene            complement(2120..4036)
                     /gene="mngA"
                     /locus_tag="ACUQPX_001636"
                     /pseudo
     CDS             complement(2120..4036)
                     /gene="mngA"
                     /locus_tag="ACUQPX_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
     gene            4206..4928
                     /gene="mngR"
                     /locus_tag="ACUQPX_001637"
     CDS             4206..4928
                     /gene="mngR"
                     /locus_tag="ACUQPX_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HHV9056378.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5032..5901)
                     /gene="sucD"
                     /locus_tag="ACUQPX_001638"
     CDS             complement(5032..5901)
                     /gene="sucD"
                     /locus_tag="ACUQPX_001638"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HHV9056379.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPEGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(5901..7067)
                     /gene="sucC"
                     /locus_tag="ACUQPX_001639"
     CDS             complement(5901..7067)
                     /gene="sucC"
                     /locus_tag="ACUQPX_001639"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HHV9056380.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(7161..8378)
                     /gene="odhB"
                     /locus_tag="ACUQPX_001640"
     CDS             complement(7161..8378)
                     /gene="odhB"
                     /locus_tag="ACUQPX_001640"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HHV9056381.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(8393..11194)
                     /gene="sucA"
                     /locus_tag="ACUQPX_001641"
     CDS             complement(8393..11194)
                     /gene="sucA"
                     /locus_tag="ACUQPX_001641"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HHV9056382.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(11495..12211)
                     /gene="sdhB"
                     /locus_tag="ACUQPX_001642"
     CDS             complement(11495..12211)
                     /gene="sdhB"
                     /locus_tag="ACUQPX_001642"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HHV9056383.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(12227..13993)
                     /gene="sdhA"
                     /locus_tag="ACUQPX_001643"
     CDS             complement(12227..13993)
                     /gene="sdhA"
                     /locus_tag="ACUQPX_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HHV9056384.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(13993..14340)
                     /gene="sdhD"
                     /locus_tag="ACUQPX_001644"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(13993..14340)
                     /gene="sdhD"
                     /locus_tag="ACUQPX_001644"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HHV9056385.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(14334..14738)
                     /gene="sdhC"
                     /locus_tag="ACUQPX_001645"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(14334..14738)
                     /gene="sdhC"
                     /locus_tag="ACUQPX_001645"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HHV9056386.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            15119..15226
                     /locus_tag="ACUQPX_001646"
     CDS             15119..15226
                     /locus_tag="ACUQPX_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056387.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            15432..16715
                     /gene="gltA"
                     /locus_tag="ACUQPX_001647"
     CDS             15432..16715
                     /gene="gltA"
                     /locus_tag="ACUQPX_001647"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HHV9056388.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            17105..17671
                     /locus_tag="ACUQPX_001648"
     CDS             17105..17671
                     /locus_tag="ACUQPX_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9056389.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            17722..20181
                     /locus_tag="ACUQPX_001649"
     CDS             17722..20181
                     /locus_tag="ACUQPX_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HHV9056390.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     IDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSDK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKVTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            20196..20927
                     /locus_tag="ACUQPX_001650"
     CDS             20196..20927
                     /locus_tag="ACUQPX_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0043711 - pilus organization [Evidence
                     IEA];
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis chaperone"
                     /protein_id="HHV9056391.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVETKK"
     gene            20924..21985
                     /locus_tag="ACUQPX_001651"
                     /pseudo
     CDS             20924..21985
                     /locus_tag="ACUQPX_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            22137..23183
                     /locus_tag="ACUQPX_001652"
     CDS             22137..23183
                     /locus_tag="ACUQPX_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HHV9056392.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(23180..23971)
                     /gene="nei"
                     /locus_tag="ACUQPX_001653"
     CDS             complement(23180..23971)
                     /gene="nei"
                     /locus_tag="ACUQPX_001653"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HHV9056393.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKKRLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(24007..24741)
                     /gene="pxpA"
                     /locus_tag="ACUQPX_001654"
     CDS             complement(24007..24741)
                     /gene="pxpA"
                     /locus_tag="ACUQPX_001654"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HHV9056394.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(24731..25663)
                     /gene="pxpC"
                     /locus_tag="ACUQPX_001655"
     CDS             complement(24731..25663)
                     /gene="pxpC"
                     /locus_tag="ACUQPX_001655"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HHV9056395.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLFHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(25657..26313)
                     /gene="pxpB"
                     /locus_tag="ACUQPX_001656"
     CDS             complement(25657..26313)
                     /gene="pxpB"
                     /locus_tag="ACUQPX_001656"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HHV9056396.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDSARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(26336..27079)
                     /gene="ybgI"
                     /locus_tag="ACUQPX_001657"
     CDS             complement(26336..27079)
                     /gene="ybgI"
                     /locus_tag="ACUQPX_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HHV9056397.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            27350..28831
                     /gene="dtpD"
                     /locus_tag="ACUQPX_001658"
     CDS             27350..28831
                     /gene="dtpD"
                     /locus_tag="ACUQPX_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HHV9056398.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(28981..30399)
                     /gene="phrB"
                     /locus_tag="ACUQPX_001659"
     CDS             complement(28981..30399)
                     /gene="phrB"
                     /locus_tag="ACUQPX_001659"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HHV9056399.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARIQTLAAYEAARKGK"
     gene            complement(30396..30905)
                     /locus_tag="ACUQPX_001660"
     CDS             complement(30396..30905)
                     /locus_tag="ACUQPX_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HHV9056400.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            31034..31363
                     /locus_tag="ACUQPX_001661"
     CDS             31034..31363
                     /locus_tag="ACUQPX_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HHV9056401.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGCLTIIADSDEVQELREELYQVKQVVKGMRDGM
                     FSVLNES"
BASE COUNT         7580 a         8503 c         7770 g         7695 t
ORIGIN      
        1 ttacgccctt cctgtaaggc tgcatagttg agcatattcc ataccggaac cggcgcttct
       61 ttccagcctt cttgctgcca gttgttcatt gcactgtcgt tcacggggcg cgttagcgaa
      121 ccaaactggg tatctgccag aacactatcg gtgttaaaag atgtagggat caggacgcga
      181 aggcgatgat cgtcagcctg gttatcaagg ttgatatcca catcaatgcg cctgttgtta
      241 tgactaagag tgacaaccaa ctcgacacct actctgccag tggattgtct tgcgctgcgt
      301 tgttgcagat tacgcggcac cgccatttca tagcggataa cagccctgct ctgccaggcc
      361 tcatgaataa tctcgcattg cggtttcgca ttcgccgaag taattaccca ctcttctttt
      421 gcgggtgaat agtcatattc atcaccatca tcagagcttt cttcaatttg caacacccgg
      481 tcatagcgca caccgctatc tttatctacc agttgtagcg ttccatcctc attgagcgca
      541 atttgccaga aagcattttc cagtatccct tcagcgtcac tttttgccgc aatcacgttg
      601 ccaggctgat tcgcttcgat ataaagcgtg cgatagccca tagaagggac aatctggttg
      661 atctgtatat caaactccat aaagggatcg taattaccgt aatgaactat ttgccgatcg
      721 attaggcctg gatcgatctc acgggcatgg cgaataaaat acggtacagg ctgaccgcga
      781 tcgtctcgta gattaaactg gctggcgcgc agccgcacag tggtgttgat cacttcttcc
      841 cgcggccagg gcatcaggtt aaacaggacg agtttgtcgg cgtcgctctg cggcatgttg
      901 tcggcgattt tacgcatata gaaacgcagc agattatccg ccatgtcttc agccagttcg
      961 aagcgggcga cgatttcgcg atgaactttg tcactacagc agcagccgat gctgtcgtgg
     1021 gcatgatttt ttaagatctc tttccacatt ttctccagca agccgtggtg gtattcaaaa
     1081 cccaacgtcc aggccagcgt tgccagcggt tccagcggtt ccagcggttc cagcggttcc
     1141 agcggttcca gcggttccag cggttccagc ggttccagcg gttccagcag attaacaatc
     1201 ttattttcaa tacgtgcgtg ggcaactttg atatccatgc gcgtggaacc gatggtgcga
     1261 tgcacgcgca tatatttgcc atcaataaat tcccctttca gggttgccag attatctcgc
     1321 tgcgcttcga tcttctcaaa tacctcttca aagcggctca tcacaaactt tcgttgagga
     1381 taaatttcgc gcagtttctc catcacttcg aagatatttt gctgcaatgg catctgatca
     1441 tgcccattcg gcagcaaaat ctctttggtt acagacgctt tttccagcac gtcaaaataa
     1501 ctgtcgaggc gtttgcgtaa tccgttttcg tccgcaggta agtacttacc gatggcataa
     1561 cccaacggca tcacctgcgc cgtcacttcg ctaccgtcac tgctttgcca caaaaactcg
     1621 gttttatccg taccgtggcg ctcagaacat ccacgccaga acatggtgca ggtaatgcca
     1681 aatccattgt agatatgcgg cagttgcccg gacatgccaa aggaatcggg taaataacct
     1741 attttcatcg gctcgccaaa cgcgaggcag tcacgcattc cgtacatcag attacggacg
     1801 atggattccg cagaaacaat cgtggtatcg gtctgggtat accaggggcc gataatcaac
     1861 ttgccggctt ctaccagttt cttcacgcgg tctttgtttt ccgatttcac cgcgaaataa
     1921 tcttcgagga tcgccgtttg cccgtcgagt acgtaatatt tgtattcgtt gtcctgttcc
     1981 agtcggcaca ggatctcttc catattattg accagcagaa tgcgtgactc ttcggtggtg
     2041 aaataccact cccgatccca gtgcatatgc ggggtgatgt gaacgcgaga taccgctttc
     2101 atcgtcgttt cctttttgtt tatggcatta cgccatcagt cagatagttg ccatgcttaa
     2161 ccgcgtgacg ccgccagatc aggagaattg cagtcgagat tgcagccccc accaatgccg
     2221 caccaaacca gccaattgcc gccataacac cgcccgcgcc attatcatga agtaaaaaga
     2281 gcgagaaaat gccagcaccg ggtgtcgaaa ggccgatatt catcgcaccg acaatagcgc
     2341 ccgttaccat agagcccagc acaaacgaac caataacccg cagcggatct tcaatcgcca
     2401 tcgggatcgc cccttcggta atacctgcca gccctaacag ccaggtggat ttcccggtct
     2461 caatttcaaa ctctttaaac aggcgcggtg cgagcatcgt ggaagcggtt acggtaaatg
     2521 ccgaaaccat tttgacggag gcgaaaatgg catacgggcc gtaaacgccg ttcgccattg
     2581 cgcccaagca gaatgcataa gcggctttat tcactggccc tccaaggtca aaggaacaca
     2641 taaaaccgag aatggcaccc agcaacagcg cgttacttcc tgacagaccg ttcagctagg
     2701 cggtaagcga gttattgatc caggcgacag gttcccccac cacaaacagc atcagactgc
     2761 ccgctcccaa tgtaccgagc accgggtaga gataaaaagt caggaagcca ttgaatttac
     2821 tgctaagacg caagtgattt ttcatccagc gtatcaagta accggctatc aatccgccaa
     2881 cgaccgcgcc gagaaacccg gagccgatca tgttggcggc aagtccagcc gcaaagcctg
     2941 gcgctaacgc cggtttatct gccagcgaat aggcggtata ggctgccagc accggtacca
     3001 tcaaaattcc gagcagcccg ccgcccagtt tgcggtacat ccacagccag gagttttctt
     3061 cattaaacag atcttgtagc ccgaagattt gcgacagtaa taccgcgacc gccagcaccg
     3121 tcccccccgc gacaatcaac ggtacggcaa aagagatccc gcccaacagt gcctgtttca
     3181 gctccgtttt gacgcttttc accggttgcg tatcttgctg tacggtacgc gtctcatcgc
     3241 cacgctcgag ggtaagcgat tgttggatca acgcttctgc atggcgaatc ggctcggcaa
     3301 cagacacaga aagcgcagga atgccgttaa aacgctcact ctccttgatg gcgacttcag
     3361 ccgcaaaaat acaggcagtt gcactattga gttgatccgc cgttaaacgc ccttcaatac
     3421 cgttagcacc ttgcttttca acgtagacat tcacgccgag tttgcgtccg gctttttcca
     3481 gatactccgc agccatgtag gtgtgagcaa tacccgccgg gcaggccgtt acgcaaacga
     3541 tagttggcgc gttggaaaaa gaggcagaag gttgcgtgcc tcccttgtca tccagcgccg
     3601 agagcaattc atcaggcgtc gtcgccgact gtatacgcgc ccgaatctca tcatccgcca
     3661 ggcgcgtggt cagcgctgtc agcagttgca tgtgcgtagt acccgcttca ttggggggaa
     3721 tcgccagcaa caccactaaa tcaaccgctt ccgggccatc cacgccttcc cactgaagcg
     3781 gctcgctgag tgtggcgacc gcaaacgccg cttcttttac cgcagcagtt ttgccatgcg
     3841 gcacagccaa cccttcacct aaggcggtcg ggccaaggct ttcacggcga tacacttctt
     3901 tcagaaattg ctcagtactg gaaattttcc ccagagcagc aagacgttga gtcaacgcgt
     3961 gaatggcctc ttcacggctg gtaaagcgcg cattcagaca caacgcatcg cggtgggtca
     4021 gagtcgtcag attcatcatg atcctcactt gatcgttttt atagtcgcgc cagtgtgccc
     4081 gataaaacaa taccattgcg tgatcaattt cacttgattg aaattattta cattgtattt
     4141 gtattattta caggatgaat ttatcgggca taatggctaa attcgtatta atgagatgct
     4201 aaataatggg acacaaaccc ttataccgac agattgctga tcgcattcgt gagcaaattg
     4261 cgcgtggcga gcttaaacct ggcgatgcgt tacctaccga atcggcatta caaacagagt
     4321 ttggcgtcag ccgggttacg gtgcgtcagg ctttgcgcca gttagtcgag caacagatcc
     4381 tcgaaagcat tcagggcagc gggacttacg tcaaagaaga gcgggtcaat tacgatattt
     4441 ttcagttaac cagttttgat gaaaaactgt cggaccgtca cgtcgataca cacagtgaag
     4501 ttctgatatt tgaagtgatt ccggctgacg attttcttca gcaacagcta caaatcaccg
     4561 tgcaggatcg tgtgtggcat gtgaagcgcg tgcgctatcg caagcaaaag ccaatggcgc
     4621 tggaagaaac ctggatgccg cttgcgttgt tcccggatct cacctggcag gtcatggaaa
     4681 attcgaaata tcactttatt gaagaagtga agaagatggt tatcgatcgt agcgaacagg
     4741 aaatcattcc tctgatgcca accgaagaga tgagccgtct gcttaatatc agccagacaa
     4801 agccgattct tgaaaaagta tcgcgtggat atttagttga tggtcgggtg tttgaatata
     4861 gccgcaacgc ttttaatacc gatgactata aatttaccct gatagcgcag cgaaaatcat
     4921 cgcgataagc acaaaaaagg ccatcatacg atggccaacc atgtcgaaac cgacagccct
     4981 tcgccattcc tgtagaggga agcggcgagg gctatttctt attacagata tttatttcag
     5041 aacagttttc agtgcttcac cgatatccgc caggctgcga acggttttca cgcctgcggc
     5101 ttccagagca gcgaatttct catccgcagt ccctttccca ccggcaatga tcgcacccgc
     5161 gtggcccata cgtttgcctt ccggcgcagt cacaccagcg atgtaaccca caactggctt
     5221 ggtaacatgc tctttgatgt acgctgccgc ttcttcttca gcgctaccgc cgatctcacc
     5281 gatcatcacg atcgcttcgg tctgcggatc tttttcgaac atttcgagaa tgtcgataaa
     5341 gttagagcca gggatcgggt caccgccgat accgacacag gtcgactgac cgaaaccgta
     5401 atccgtggtc tgtttaaccg cttcataggt cagtgtaccg gaacgggaaa cgatacccac
     5461 tttacccggt ttgtgaatgt gacccggctg gataccgatt ttgcattcac ccggggtgat
     5521 aacgcctggg cagttcgggc cgatcatacg aacgcctgct tcgtccagtt tcactttcac
     5581 ggtcagcata tccagcgtcg ggatgccttc agtgatggtg ataatcagtt tgatgcctgc
     5641 atcgatagct tccagaatgg agtctttgca gaacggtgct ggtacgtaaa taacagacgc
     5701 ggaagcgcca gtagcagcaa cagcttcacg cacggtgttg aacaccggca ggccgaggtg
     5761 ggtagtgccg cctttacctg gagttacgcc accaaccatt ttagtgccgt atgcaatggc
     5821 ctgttctgag tggaaagtcc cctggctacc ggtaaagccc tggcagataa ccttggtgtt
     5881 tttatcaatt aaaatggaca ttatttcccc tccactgcgg caacaacctg ctgagctgca
     5941 tccgtcagac cttttgctgc aataatattc aggccgctgt cagccagttt cttcgcgccg
     6001 agttcggcgt tgttaccttc cagacgtacc acgaccggta cgttaacacc cacttccgct
     6061 accgcgccga tgataccgtc agcgatcagg tcgcaacgaa cgataccgcc gaagatgtta
     6121 accagaacgg ctttcacttt gtcgtcagag aggatgattt tgaacgcttc ggttacacgt
     6181 tctttggttg cgccgccgcc aacgtccagg aagttagccg gttcgccgcc gtgcagttta
     6241 actatgtcca tcgtacccat cgccagacct gcgccgttaa ccatacaacc gatgttaccg
     6301 tccagcgcaa cgtagttcag ttcccactgt gcagcctgtg cttcacgcgg atcttcctgc
     6361 gactggtcac gcatttcgcg cagatcaggc tggcggaaca gggcgttgcc atcagcgccc
     6421 agtttgccgt cgaggcaaat cagatcgccc tgtttggtga tgaccagcgg gttgatttcg
     6481 atcagcgcca ggtcgcgctc caggaaaatg gtcgccaggc ccatgaagat tttggtgaac
     6541 tgctgaacca gtttaccttc cagacccagt ttgaacgcca gctcgcgtcc ctgatacggc
     6601 atcgggccag tcagcggatc aagcgcaact ttatggatca ggtgcggagt ttcttccgcc
     6661 actttttcga tttccacgcc gccttcggtg gaggccataa agaccacgcg acgggaacta
     6721 cggtctacaa cggcaccgag atacagctct ttagcgatat cggtcgccgc ttcaaccaga
     6781 atctggttaa ccggttggcc attggcatct gtttgatacg ttaccagacg cttgcccagc
     6841 cagttttctg caaaagcacg gatgtcttct ttgctgttta caactttcac accgcccgct
     6901 ttaccgcggc caccagcgtg aacctgacat ttcactaccc acggaccggc accgattttt
     6961 gaagcggctt cttctgcttc gcgcggagta gtacaggcat aacccaccgg tgctggtaag
     7021 ccatagcggg caaacagttg ttttgcctga tattcatgta agttcatgtg ttctgtccat
     7081 cctccagtaa tcgttatctt ttaaaccgta gaccgacacc ctatccacgt cggtctacag
     7141 tgcaggtgaa gcttaaacta ctacacgtcc agcagcagac gcgtcggatc ttccagcaac
     7201 tctttgatcg ttaccaggaa gcccacggat tcgcgaccat cgatcagacg gtgatcgtag
     7261 gacagcgcca ggtacatcat cggcaggatc tcaacctgac cattcaccgc catcggacga
     7321 tctttgatag cgtgcatacc cagaattgcg ctctgcggcg ggttgatgat cggcgtagac
     7381 atcagggaac cgaacacacc accgttggtg atggtgaagt taccaccggt cagatcttca
     7441 accgtcagct tgccatcacg gcctttgact gccagctctt tgattttctt ctcgatgtct
     7501 gccatgccga gggtatcgac atcacgcaga accggcgtca ccaggccgcg cggcgtagaa
     7561 accgccatgc tgacgtcgaa atagttgtgg taaaccacgt catcgccgtc gatagacgcg
     7621 ttcacttccg ggtaacgttt cagggcttca accaccgctt tcacgtagaa ggacataaag
     7681 cccagacgga tgccgtggcg tttttcaaac gcttcaccgt actgcttacg cagatccata
     7741 atcggcttca tgttgacttc gttgaacgtg gtcagcatgg cggtggagtt tttcgcttcc
     7801 agcagacgct ctgccacacg cttacgcagg cgagtcatcg ggacacgttt ttcactacgt
     7861 gcagccagag ccggttgcgc cgccggagca gccgctgccg gagcagactc tttcgccggg
     7921 gctttcgcca gatgtttttc cacatcttca cgagtcagac gaccacccac accggtgcct
     7981 ttaatggcgc tggcgtcgag attgtgttca gccagcaggc gacggatcgc cgggcttaac
     8041 gcatcgttgt tttgctcttc cagagacgcc tgctggcgtt gcgccggagt ggacgctttc
     8101 tcttcagatt tggcgctggt ttctttaccg gcgctgttgc cttcacgcag gcgaccaagg
     8161 atctgacgag acgttaccgt tgtaccttca tcttccagaa ccgcatccag aatgccgtct
     8221 gctgatgccg gtacttccag taccactttg tcagtttcga tttctaccag cacttcatca
     8281 cgtacgactg cgtcgccggg ttttttatgc caggttgcga cggtggcatc ggctacggat
     8341 tcaggcaggt cagggaccag aatatctacg ctactcattg tgtatccttt atttattcga
     8401 cgttcagcgc gtcattaacc agatcttgtt gctgtttctg gtgaacggac atatacccta
     8461 ccgccggaga ggcggaggcc gggcggcctg cataacgcag agaagcccca aacggaatca
     8521 cttcacggaa atgatgctgg ctgcagtacc atgcgccctg gttgagcggc tcttcctggc
     8581 accagacaaa atccttgacg tgagcaaact gctgcaacac ttcctgcatc gctttatgcg
     8641 ggaacgggta gagttgctcg atacgcacaa tggcgacatc gtgttgattg ttcttacgac
     8701 gctgttccag caggtcgtaa taaaccttac cagaacacat cactacgcgc ttcacgccct
     8761 tcggatcaag ctcgtcgatt tcaccgatgg ctggcaggaa ggtgccgttc gccagttctt
     8821 cgaggctgga aaccgccagc ggatgacgca gcagggattt cggcgacatc acgaccagcg
     8881 gacgacgcat cccgcgcagc gcctgacgac gcagcatgtg gtaaacctgt gccggggtag
     8941 acggtacgca aacctgcatg ttttgctcag cacaaagttg cagataacgt tccagacgcg
     9001 cggaggagtg ctccggcccc tgcccttcgt aaccgtgcgg cagcaacatc accagaccac
     9061 acatccggcc ccatttctgc tcgccagagg agatgaactg gtcgataacc acctgcgcac
     9121 cgttggcgaa gtcaccgaac tgcgcttccc agatggtcag ggtgcgtggt tctgcggtgg
     9181 cataaccata ttcaaacgcc agcacggctt cttctgacag tacggagtcc cagacacgga
     9241 acgcgccctg accgttatgg atgtgttgca gcggcgtgta agtggaaccg ttagactggt
     9301 tgtggatcac cgcgtggcgg tggaagaagg taccgcgacc ggagtcttca cccgacaggc
     9361 gaaccggaat gccttcatca accagcgtgg cgtaagcgag gttttccgca ccgccccagt
     9421 cgaacagttt ctcaccggct gccatcgctt ggcgatcgcc ataaatcttg gcaacgcgag
     9481 actgcatttc aactgcttct ggtaccgtac tgatacgttt agccagttcc tgcaggcgct
     9541 tcatctcaac tttgttcggg tactcttcgt cccattcgtg gttgaggtac ggcgaccagg
     9601 tgaaagagtg catgttcatc ggacgccact ctgccactac gcaatcgcca gcatccagcg
     9661 catcgcggta caggttaacc atctcggtgg catcttccag cgtcgccact ttttcctgct
     9721 ccagcttgtc agcgtagatt ttgcgcggtg tcggatgttt tttgattttc tgatacatca
     9781 gtggctgggt tgcgctcggc tcgtcggctt cgttgtggcc gtgacggcgg tagcatacca
     9841 ggtcgatgaa gacatcacgt ttaaaggtgt tacggaaatc gagcgccaga cgggtcacaa
     9901 aggcaacggc ttccggatca tccgcgttaa cgtggaaaat cggtgcctga accatcttac
     9961 cgatatcagt acagtacggt gtagaacggg catccagcgg gttagaggtg gtgaaaccaa
    10021 cctggttgtt gataacgata cgtaccgtac cgccaacttc ataaccacgc gctttcgaca
    10081 tgttcagagt ttcctgaacc acgccctgcc cggtcactgc ggcgtcaccg tggatggtga
    10141 ttggcagcac tttgttgctg ctcggctcat caagtctgtc cagacgggca cgaacagagc
    10201 cgataacgac cgggcttaca atctcaaggt gagacgggtt aaacgccagc gccaggtgca
    10261 ccaggccgcc atcggtctgg aagtcagacg agaagcccat gtggtatttc acgtcgcctg
    10321 tgccgaggtg ttctttatgt ttaccggcga actcgtcgaa caagtcttgc ggttttttac
    10381 ccagcacgtt caccagcacg ttcagacgac cacggtgcgc catcccgaga accacttcgc
    10441 gggtgccgct gttgccagcg tggcggatca tctctttaag catcgggatt aacgcgtcac
    10501 cgccttccag cgagaagcgt tttgcgccag ggaattttgc gccgaggtaa cgctcaaggc
    10561 cttcagcggc ggtcagttcg cttaagaagc gttttttctc ttcgctattg aaagtcgcgc
    10621 gaccagactc gatacgctgt tggatccagc gtttttcttc ggtgctggtg atgtgcatat
    10681 actcggcacc aatcgggccg cagtaggttt gcttgagggc ggaaataagc tcgccgagtt
    10741 tcatggtttc tttgccgctg gcaaatgaac cgacgttgaa ggtttcctgg aagtctgctt
    10801 cggtcagatc gtggaaagac ggatccagat cggccacttt atcttgctgc cacagtccca
    10861 gcggatctag attcgcatgc tggtgaccac ggaagcggta tgcgttaatg agctgcagga
    10921 ctttaacctg cttcacattg gtgtcagggt cggagatcgt tgaagagtaa cgtgaagcgt
    10981 ctttcgccag gcggcggaaa tattcacgcg tttgagagtg gaattgatcc ggtttgactc
    11041 ccgtaccagg taactgctgg aacgtcgaac gccagttagc gtcaaccgag tcaggatcgg
    11101 ttaagaagtc ttcatagagc tgttctatcc agctctggtt tgcgccagag aggtaagaag
    11161 agtccaacca ggctttcaaa gcgctgttct gcatcgtgat cccttaagca tcttttttat
    11221 gcttacttcg ccgtggatac taccacgcac agtgatgtgc gtgccggggt tcacttgcga
    11281 gctcttgtct acgtagtggc tcgcgaagga acctttaaaa actgtcttag tgtcaggggc
    11341 agtttttaaa gatttcctgc atttctttgt tacgcctgat gcgctgcgct tatcaggcct
    11401 gcgggtaatg acttgtaggc cggataaggc gttcacgccg catccggcac tggttgcctg
    11461 atgcaacgct tgcgcgtctt atcaggccta cagtttacgc attacgttgc aacaacatcg
    11521 acttgatatg gccgatggcg cgcgtcgggt tcagcccctt cggacataca ctgacgcagt
    11581 tcatgatgct gtgacagcgg aatacgctga atgcatcgct caaaccgtcg aggcggctgt
    11641 cagtctcggt atcacggcta tcgatcagga aacgatatgc cgctaacaag cctgccgggc
    11701 cgataaactt atcgggattc caccagaaag acggacaaga ggttgaacaa catgcgcaga
    11761 gaatacattc atacagcccg tcgagttttt cgcgctgctc tggcatctgt aaatgctcgc
    11821 gagctggcgg attttgtcca ttattcaaca ggtaaggctt aattttctca tattgcgcat
    11881 agaattgtcc catgtctacc accaaatcgc ggatcaccgg taaacctggc agcgggcgaa
    11941 tcacaatctt cttgcccggc tggttgagtg ccgaaatcgg ggtaatacag gccagaccat
    12001 tcttgccgtt catgttcaga ccgtcggaac cgcacacacc ttcacggcag gagcggcgga
    12061 acgacaggct gggatctttc tctttcagct ggataagcgc atccagcagc atcatgtcac
    12121 gaccttcttc cgcttccagg gtgtaatcct gcatacgcgg agcatcatca acatccgggt
    12181 tatagcgata aattgaaaac tcgagtctca ttttcctgtc tccgcattag taagtacgaa
    12241 tcttcggcgg gaatgccggg cgcagtttcg gttccatgtt gacgcttcgg cgcgtcatgg
    12301 attccgactc aggcagatac agggagtggc acagccagtt ttcatcatca cgatccggga
    12361 agtcgaagcg gctatgtgcg ccacggcttt cggtacggaa gttggcagaa acagccgttg
    12421 catacgccgt ttccatcagg ttatccagtt ccaggcactc aacgcgctgg gtgttgaact
    12481 cgctggaagt gtcatccaga cgggcatttt tcagacgctc gcggatcact ttcaactgct
    12541 caagcccttt cgccatcgca tcaccttcac ggaagaccga gaagttatgc tgcatacatt
    12601 cttgcagcgc tttacggatc gccaccggat cttcaccgtt acgattattg ttccagcggt
    12661 tcaggcgatc cagagacgct tcaacatcag actcgctggc atcgcgcagt gcgccctgct
    12721 cggcgataga ctcttgcaga tgcagacctg ccgcgcgacc aaagaccacc aggtccagca
    12781 gcgagttgcc gcccagacgg ttagcgccgt gtaccgatac acaagcgatt tcaccaacgg
    12841 caaacagtcc cggaacaacc acatcttcgc ctttctcatt cacagtcagc gcctgaccgg
    12901 taactttggt cggaataccg cccatcatgt agtgacaggt tgggataacc ggaatcggct
    12961 ctttcaccgg atcgacgtga gcgaaggtac gggaaagctc caggataccc ggcagacggg
    13021 attcgagaac ttctttaccc aggtgatcga gtttcagttt cgcgtgtggc ccccacggac
    13081 catcacagcc gcgaccttca cggatttcaa tcatgatgga acgcgccacc acatcacggc
    13141 ccgccaggtc tttggcgttc ggcgcataac gctccataaa gcgttcgcca tgtttgttca
    13201 gcagataacc gccttcacca cggcaacctt cggtgaccag tacgcccgca ccggcaatgc
    13261 cggtcgggtg gaattgccac atttccatat cctgcaccgg tacgcctgcg cggatagcca
    13321 tgccgacacc gtcgccagtg ttaatgtggg cgttagtggt ggactgataa atacgccctg
    13381 ccccgccagt cgccagcacg gtagcgcggg ctttgaaata aaccacttca ccggtttcga
    13441 tgcacagtgc ggtacaaccc accaccgcgc catcctggtt tttcaccaga tccagcgcat
    13501 accactcgga gaaaatggtg gtgtggtttt tcaggttctg ctgataaagc gtgtgcaaca
    13561 gtgcgtgacc ggtacggtca gcagccgccg cagtgcgtgc cgcctgctcg ccgccgaagt
    13621 ttttcgactg accgccaaac ggacgttgat agatacgacc atcatcgaga cgcgagaacg
    13681 gcaggcccat gtgttcgagt tccagaatcg cttccggccc ggttttacac atatattcaa
    13741 tcgcgtcctg gtcaccgata tagtccgacc cttttacggt gtcgtacata tgccattccc
    13801 agttatcttc atgggtatta cccagcgcaa cggtaatgcc gccttgcgca gaaacggtat
    13861 gggaacgggt cgggaagact ttagagagca gcgcacaggt ctggccgctc tgggaaattt
    13921 gcagcgccgc gcgcatacct gcgccaccgg caccaatcac aactgcatca aattctctga
    13981 ctggcaattt catcacacac cccacaccac aacgaatcca taaatcacgt aaaccaccag
    14041 tgcaacgaca atcaccagtt gcagcatcag gcgcaaggcc agcggtttaa cgtagtcggt
    14101 caacacctgc cacatgccga tccaggcatg aatcaagata gaaaacagcg ccagcagggt
    14161 gaacactttg gtgaacgcag aggcgaagaa accgatccag acttcatatg tcagttcgcc
    14221 actggtggcg aaaaaaccga ccatataaat gatgtagagc gtcaggacga tagcggtagc
    14281 gcgaacgagg atgaaatcat gtacgccatt gcgtcctaat gcggaggcgt tgcttaccat
    14341 acgaggactc ctgcgagaag tgaaagcacg acagtaataa caaaggagat tttggcggag
    14401 cgtttacccg cttcgaatgt ttcttccaga tagccaaaat ccatcatcat gtggcgaata
    14461 cctacgacga cgtgatacgc cagagcggta aggatgcccc acatgataaa tttgacgaag
    14521 aagctgccca taatcgcgga agcttgctcg aaaccttcag gggaagagag gctggtaccc
    14581 agaagccaca gcaggatgcc cactgcaaca aaggtgatca caccggaaac gcgatggaga
    14641 atggacgcta tcgccgtgat ggggaaccgg atggtctgta ggtccagatt aacaggtctt
    14701 tgttttttca catttcttat catgaataac gcccacatgc tgttcttatt attccctggg
    14761 gactacgggc acagaggtta actttctgtt acctggagac gtcgggattt ccttcctccg
    14821 gtctgcttgc gggtcagaca gcgtcctttc tataactgcg cgtcatgcaa aacactgctt
    14881 ccagatgcga aaacgacacg ttacaacgct gggtggctcg ggattgcagg gtgttccgga
    14941 gacctggcgg cagtataggc tgttcacaaa atcattacaa ttaacctaca tatagtttgt
    15001 cgggttttat cctgaacagt gatccaggtc acgataacaa catttattta atttttaatc
    15061 atctaatttg acaatcattc aacaaaattg ttacaaacat tactaggaaa agcatataat
    15121 gcgtaaaagt tatgaagtcg gtatttcacc taagattaac ttatgtaaca gtgtggaagt
    15181 attgaccaat tcattcggga cagttattag tggtagacaa gtttaataat tcggattgct
    15241 aagtacttga ttcgccattt attcgtcatc aatggattct ttacctgcaa gcgcccagag
    15301 ctctgtaccc aggttttccc ctctttcaca gagcggcgag ccaaataaca aacgggtaaa
    15361 gccaggttga tgtgcgaagg caaatttaag ttccggcagt cttacgcaat aaggcgctaa
    15421 ggagacctta aatggctgat acaaaagcaa aactcaccct caacggggac acagctgttg
    15481 aactggatgt gctgaaaggc acgctgggtc aagatgttat tgatatccgt actctcggtt
    15541 caaaaggtgt gttcaccttt gacccaggct tcacttcaac cgcatcctgc gaatctaaaa
    15601 ttacttttat tgatggtgat gaaggtattt tgctgcaccg cggtttcccg atcgatcagc
    15661 tggcgaccga ttctaactac ctggaagttt gttacatcct gctgaatggt gaaaaaccga
    15721 ctcaggaaca gtatgacgaa tttaaaacta cggtgacccg tcataccatg atccacgagc
    15781 agattacccg tctgttccac gctttccgtc gcgactcaca tccaatggca gtcatgtgtg
    15841 gtattaccgg cgcgctggcg gcgttctatc acgactcgct ggatgttaac aatcctcgtc
    15901 atcgtgaaat tgccgcgttc cgcctgctgt cgaaaatgcc gaccatggcc gcgatgtgtt
    15961 acaagtattc cattggtcag ccatttgttt atccgcgcaa cgatctctcc tatgccggta
    16021 acttcctgaa tatgatgttc tccacgccgt gcgaaccgta tgaagttaat ccgattctgg
    16081 aacgtgctat ggaccgtatt ctgatcctgc acgctgacca tgaacagaac gcctctacct
    16141 ccaccgtgcg taccgctggc tcttcgggtg cgaacccgtt tgcctgtatc gcagcaggta
    16201 ttgcttcact gtggggacct gcgcacggtg gtgctaacga agcggcgctg aaaatgctgg
    16261 aagaaattag ctccgttaaa cacattccgg aatttgttcg tcgtgcgaaa gataaaaatg
    16321 attctttccg cctgatgggc ttcggtcacc gcgtgtacaa aaattacgac ccgcgcgcca
    16381 ccgtaatgcg tgaaacctgc catgaagttc tgaaagagct gggcaccaaa gatgacctgc
    16441 tggaagtggc tatggagctg gaaaacatcg cgctgaacga cccgtacttt atcgagaaga
    16501 aactgtaccc gaacgtcgat ttctactctg gtatcatcct gaaagcgatg ggtattccgt
    16561 cttccatgtt caccgtcatt ttcgcaatgg cacgtaccgt tggctggatc gcccactgga
    16621 gcgaaatgca cagtgacggt atgaagattg cccgtccgcg tcagctgtat acaggatatg
    16681 aaaaacgcga ttttaaaagc gatatcaagc gttaatggtt gattgctaag ttgtaaatat
    16741 tttaacccgc cgttcaaatg gcgggttaat ttttatatgc ctaaacacaa aaaattgtaa
    16801 aaataaaatc cactatcgga cattcatata ttctaaaaaa aatatatcgg tataaattat
    16861 aaacaaatcg atgattttta atcatctatc ttcctctcag ttacatgaat aaaccaccaa
    16921 atatagcgca cccctataat ttccaatact ttattcatca aacatttaat tcataccatc
    16981 aattaattac ttttacattt aataaaatta ataatatttt taagaaaaac taaaaacatt
    17041 cgacaatcaa ataagatatt cttgcaccga tatacttttt tcttttctgt taaaggactc
    17101 agttatgttt aaaggacaaa aaacattggc cgcactggcc gtatctctgc tgttcacagc
    17161 acctgtttat gcggctgatg aaggatccgg tgaaattcac tttaaaggtg aagttattga
    17221 agcaccgtgt gaaatacatc aggatgatat tgataaagag gttgaactcg gtcaggtgac
    17281 caccagccac attaatcagt cacatcacag cgatgccgtt gctgtcgacc tgcgcttagt
    17341 caactgtgat ctggaaaact ccagcaacgg ttccggtggc aagatttcta aagttgcagt
    17401 cacctttgat agctcagcga aaaccaccgg cgcagatccg attctcaaca acaccagcac
    17461 gggtgaagcc accggcgtcg gcgtacgttt aatgaataaa gatcaaagca atatcgtttt
    17521 aggcaccgct actccagata tcgacctggc tccgacctcc agcgaacaaa cgctgaattt
    17581 ctttgcctgg atggaacaaa ttgatcaggc tacacctgta acgccaggcg cagttacggc
    17641 gaatgcaacc tacgtgctgg attataaata agtattactt tggcggcgct atcgccgcct
    17701 ttcataaaat atttatcagg tatggacacc gtgaatattt atcgactctc ttttgtatcc
    17761 tgcctggtcg tggcgatgcc ttgcgcattg gcggtcgaat tcaatctgaa tgttctcgat
    17821 aaatcaatgc gcgaccgcat tgatatttca ttattaaagg aaaaaggagt cattgctccc
    17881 ggtgagtatt ttgttagcgt tgcggtaaat aataaccaaa tcagtaacgg gcaaaagatt
    17941 aactggcaca aaaatgacga taaaaccatt ccgtgcatca atgatttact ggtcgataaa
    18001 tttggcttaa aacctgaagt ccgtcagtcg ttaccattga taaatcagtg cgtcgatttt
    18061 agctcccgac ctgaaatgct cttcaatttc gatcaagcca atcagcaact gaatattagt
    18121 attccgcaag cctggctggc gtggcactca gaaaactgga ccccaccctc cacatggaaa
    18181 gaaggtgtcg ccggtgtcct gatagattac aacttgtttg ccagcagcta ccgcccacag
    18241 gatggcagca gcagcactaa tcttaacgcc tatggaacag ccggaatcaa cacgggggca
    18301 tggcgcttac gcagtgatta tcagttgaat catactgata gcgatgataa ccatgaacag
    18361 tcaggcgaaa tatcacgtac ctatcttttt cgcccgttac cgcaattagg ttcaaaatta
    18421 accctcggcg aaacggattt tagttccaat attttcgacg gtttttctta taccggcgcg
    18481 gcactggcaa gtgacgatcg aatgttgcca tgggaactac gcggctacgc cccacaaatt
    18541 agcggtattg cacagaccaa tgccacggtg acgatcagtc aatcaggccg cgtcatttac
    18601 cagaaaaaag tcccaccagg cccatttatc attgacgacc ttaatcagtc tgttcagggc
    18661 acactggatg tcaaagtgac ggaagaagat ggtcgggtga acaatttcca ggtttcagca
    18721 gcatcgacgc ccttcctgac tcgtcaggga caggttcgct ataaactggc cgcgggtcag
    18781 ccacggccct ccatgtcaca tcaaactgaa aatgaaacct tttttagcaa tgaagtttcc
    18841 tgggggatgc tgtcaaacac ctcgctgtac agcggcctgc tgctttctgg tgatgactac
    18901 cattctgccg caatgggtat tgggcaaaat atgctgtggc ttggtgcgct gtcgtttgat
    18961 gtcacgtggg ccagtagcca ttttgatact cagcaggacg agcggggctt aagctaccgt
    19021 tttaattaca gcaaacaagt ggatgccact aacagcacga tttccctcgc cgcttatcgt
    19081 ttctccgatc gtcattttca cagctacgcc aactatctgg atcacaaata caacgacagc
    19141 gatgcgcagg acgaaaaaca gacgatcagc ttatctgtgg gccaaccgat taccccacta
    19201 aacctcaatc tttacgccaa cctgctacat caaacctggt ggaatgcaga cgcctccacg
    19261 accgccaaca tcacggcagg ttttaatgtt gatattggtg actggagaga tatctctatt
    19321 tcgacgtcat tcaatacaac ccattacgaa gataaagatc gcgacaacca gatttacctg
    19381 tcgatttcgc tccccttcgg taacggtggt cgggttggct atgacatgca aaacagtagc
    19441 cacagcacca cacaccgcat gtcgtggaac gatacgctgg atgaacgtaa tagctggggc
    19501 atgtctgccg gactgcaatc cgaccgtcct gacaatggag cccaggtgag cggtaactat
    19561 cagcacctga gttcagcggg tgagtgggat atttctggta cctatgccgc caatgattac
    19621 agttccgtca gcagcagctg gagcggttct ttcaccgcaa cccaatatgg tgcagcgttt
    19681 catcgccgca gctccaccaa tgaaccacgc ctgatggtca gcaccgatgg cgtggcagat
    19741 attccggttc agggcaatct cgactacacc aaccattttg gcattgcggt ggtgccgttg
    19801 atttccagtt accagccttc caccgtggcg gtgaacatga atgacttacc cgacggcgta
    19861 acagttgcag aaaacgttat caaagaaacg tggattgaag gcgcgatagg ttacaaatca
    19921 ctggcttccc gttccgataa agacgttaac gtcatcattc gcaacgccag cggccagttt
    19981 cctcccctcg gagcggatat ccgccaggat gacagcggca ttagcgtggg gatggttggc
    20041 gaggaaggac atgcctggtt aagcggagtc gctgaaaatc aaaaggttac cgtggtctgg
    20101 ggtgatagcc agcattgctc gctccatctt cctgaacata tggaagacac cgcaaatcgc
    20161 ctgattttac cttgtcatta attaaggaaa tgaaaatgac atttatgaaa ggactacccc
    20221 ttttgttgtt ggttgccagt ctgtgcagcc acgctgcact acaacccgat cgcactcgta
    20281 ttgtgtttaa cgctaatgat aaagctacca gtctgcgggt ggataatcgt agcgataaac
    20341 tcccctacct cgcttattcc tggctcgaaa atgaaaaagg ggagaaaagc gatgatcttc
    20401 tggtcgccct gccgccgata caacgtctgg aaccgaaagc aacaacgcag gtacggatag
    20461 taaagcaagc ctcgaccaca aaattaccgg gcgatcgcga aacgctattt ttctacaata
    20521 tgcgtgaaat tcctcccgcc ccggaaaaaa acagcgacca tgccgttctg caagtcgcca
    20581 ttcagagtcg tatcaaggtg ttctggcgac cggctgcatt acgcaagaaa gcaggagaaa
    20641 aggtcgaact gcagttacag gtcagccagc agggcaacca actgacactg aaaaatccta
    20701 ccgcgtatta tctgaccatt gcctacctgg gacgaaatga aaaaggcgtt ctccccggct
    20761 tcaaaaccgt gatggttgcg ccttttagta cggtcaacac aaacaccgga aattatagcg
    20821 gcagccagtt ttatctcggt tatatggatg attacggcgc gttacgcatg acgacgctca
    20881 actgcagcgg acaatgccat ttacaagccg tggagacgaa gaaatgagtg ctggcaaggg
    20941 agtgttgctc gtcatctgtc tgttatttct gccgttgaag tctgcgcttg ccctgaactg
    21001 ctactttggt tcatccggtg gctcggtaga aaaatcagaa gcgattcaac cttttgctgt
    21061 accaggcaat gccaaactcg gcgataagat ctgggaatct gacgatatta aaattcccgt
    21121 ctattgtgac aacaatacca acggcaactt tgaaagcgaa catgtctacg catgggtaaa
    21181 cccctacccg ggggtgcagg atcgttatta tcaactcgga gtaacttaca atggcgttga
    21241 ttatgacgcc aactagggaa aaagccgcat cgacactaac cagtgtatcg acagtaagaa
    21301 cattgacatt tatacccctg agcagatcat cgcgatggga tggcagaaca aaatttgctc
    21361 gggtgatccc gctaatattc acatgtcgcg tacctttctc gctcgcatgc ggttatacgt
    21421 caaaatacga gaaatgccgc cgcatgatta tcaaagtacg cttagcgact atatcgtcgt
    21481 gcaatttgac ggtgccggta gcgttaacga agaccctact gcccaaaacc tgaaatatca
    21541 tattactggt ctggaaaaca ttcgcgtgct ggattgcagc gtcaattttt ccatttcacc
    21601 ggaaacacag gtgattgatt ttggtaaatt taatttgctg gatattcgtc gccacacaat
    21661 gtcgaaaaca ttcagtatta agacgacaaa atcacaaaat gaccaatgca ccgacggatt
    21721 taaagtcagt tcctcctttt ataccgaaga aacgttggtt gaagaagata aagcattgtt
    21781 gattggtaat ggtctgaagc tacgtttgct ggatgaaaac gcctcgcctt ataccttcaa
    21841 taaatatgct gaatacgccg atttcaccag cgacatgttg gtctacgaaa aaacctatac
    21901 agctgaactt tcgtccattg caggtacccc catcgaggct gggcccttcg atacagtggt
    21961 gctttttaaa attaactata actgatttgc catgatgccc tttgcagccg gaaagggcat
    22021 ctctttcaga aaattgctga ttttcccctt ttccttgatt gctgttattt tttcctagcc
    22081 cggctttctt tatgctgtat cgatacacca aagagaaaat aatgagggag cgaaggatgc
    22141 cagttttgca gtggggaatg ttatgtgtgc tatcacttct cctttctatt ggtttcctcg
    22201 cggtacatct cccggcggcg ctattacttg ggccgatgat cgccgggatc atcttcagta
    22261 tgcgcggaat tactctgcaa cttccccgct ccgcttttct cgccgcgcag gccattcttg
    22321 gctgcatgat tgcgcaaaac ctcactggtt cgattctcac cacgctggcc gccaactggc
    22381 cgatcgtgct tgctatttta ctggtgacgc tgctttccag cgccatcgtg ggttggttat
    22441 tggtgcgcta tagctcactg cctggaaata ccggtgcctg gggttcttct ccaggcggcg
    22501 cagcggcaat ggtcgctatg gcgcaagatt acggcgcaga tattcgcctg gtggcgttta
    22561 tgcaatatct gcgagtgctg ttcgtcgcgg gggccgctgt tctggttacc cgcatgatgc
    22621 tgggtgataa cgctgaagcg gttaaccagc aaatcgtctg gttcccgcct gtgagcatta
    22681 atctcctgct tacgattttg ctcgcagtcg ttgccggtac ggcgggatgc atgctgcgtc
    22741 ttccttccgg cacgatgctc atcccgatgc tggcgggcgc agtgctccag tctggtcagc
    22801 tcatcaccat cgaactaccg gaatggctgc tggcgatggc gtatatggca attggctggc
    22861 ggattggtct tggtttcgat aaacaaatct tactgcgggc actacgcccg ctaccgcaaa
    22921 tcctgctgtc gatttttgct ctgctggcta tttgtgcagg tatggcgtgg ggactgaccc
    22981 ggtttatgca tattgatttt atgactgcct acctcgccac cagtcccggc gggcttgata
    23041 ccgtggcggt catcgccgca gggagcaatg ccgatatggc gcttattatg gcgatgcaaa
    23101 ccctgcgcct gttcagtatt ttgctgacgg ggccagccat tgcgcggttt atttcaacct
    23161 atgcgccgaa gcggtcggcc tagtgctggc agcctggaca ccagtaaaac gggcgcgagg
    23221 acaacgtggt tttctcaatg atgccgccac agcgttcgca cagttcgcca tcacgatgaa
    23281 aaaccttaaa gcgaaacagc gccccatgat gcttattctc atccacctgc ccccgcgtag
    23341 cgtaagaaaa ccgaggaatc tccagtaacg cgtgtgcgag tgcatccagt tgcgccgcat
    23401 tgagatcttt cgctttatga tttccagtca acccaacctg ccagaggatc tccacccgca
    23461 aataattgcc aagcccagcc agaaacgcct gatcgagcag taatcctgca aactgacggt
    23521 tacgaaagcg cggcgacaat aaccgtttct ttaccacctc cggcgtcaga ttcggatcca
    23581 gcacatcggg accaacgcgt tgtaaaaacg gatgcgtggt cagttgttcc ggcctcaaca
    23641 tctcaatatc cgaggcgcta taaagcagaa tggttttgtc agccgtttgc agttttaccc
    23701 gcaatactcg cgtggtctgc ggctcttcgc cggtatcaac cacgcgccag acgccgtaaa
    23761 gctgattatg gctgtagagc gttaaaccgt tgggaaaatg agttaacaac gccttaccac
    23821 gcgtttccac atgggtaacg tgttgaccga taagttgtga ttgataagtt tttaactgcg
    23881 ggaaggcaaa ccagacatca gttagtggtt tgcctttgat cgccgcctcc aggttatccg
    23941 ctgcacggcg gatctccggg ccttcaggca tggtgatatc cttttttcat tctttattaa
    24001 gcggggttat gctgcgacaa caatcccctt tttggcaaat gtagctcgta atcggcgggc
    24061 gaacgccaga gcgtgctcgc cgtcgccatg caggcagacc gtttgcgccg cgaccgttgc
    24121 ccattcgccg gtgatacttt tgactctgcc gtgttgcacc atctccagcg tttgcgccaa
    24181 cgcctgttct tcgttttcaa tcaacgcgcc tggctgactt cgcggcacca gcgagccgtc
    24241 agcctgataa ccgcgatcgg caaacacttc ctcgcgcgtt gtcaggccat attgcttgcc
    24301 tgcacgaatc agctcgcttc ctgccagccc aacgagaacc aatgccggat cgcaagcgta
    24361 taccgctctg gcgatggcgt ctgccagttg tgcttctttc gccgcctggt tgtacaacat
    24421 gccgtgcggt ttgacatgac gcattacgcc gccttgcgca cgggcaatcg ttgccagcgc
    24481 gccaatttga tacagcgtct gggcgtaaac ggtttccggc ggcagctgca tggcgctgcg
    24541 accaaaattt tccctgtcgg gaaaactcgg gtgagcgcca atcgcgacac cattttttat
    24601 tgcttcacgc acgcaagcct gcatggtttg cgcatcgcct gcatgaaatc cacaagcaat
    24661 attggcagag gaaaccagcg ttaatagctc tgcgtcgctg gcgcagcctt cgcccagatc
    24721 ggcgttcagg tcaattttca ttgtgcagcc gccacgctaa ttgttcaaaa taacgttgct
    24781 gatcttgccg cgctttcagt gcctcttcca gtgaacactg gacaaaatga atcggctgac
    24841 cgagcggaat ttgcgccaga tggtacatat cagcctcaat gatacaggca atacgtggat
    24901 aaccaccggt ggtctgtgcg tcgttcatca acacaatcgg ctgcccgtta tgcggcacct
    24961 gcaccacgcc cggtaacaaa ccgtgaaata acagttcgcg atcggtgatg cgttttaaaa
    25021 tttgcccctg taagcgatag cccattcggt tactttgcga gctaagctgc cagggcgaac
    25081 gccagaatgc atcctgcgag gcgcgatcga actcatgata ttccggcccc ggcaaggcgc
    25141 gaatgcggtt gccccacagc agctgtttaa cgccctgcgc ttccataaaa tcacgcttcg
    25201 atttgccaat cgggagtcgg tcaccatcct tcagtaaacg gccttccagc ccgccaatcc
    25261 ccactttgag atcggtgctg catgacccca ttaccggcgg aacatcaata ccacccgcga
    25321 ccgccagata actgcgcatc ccgtgctgcg ggcgtttaag cgttaaacgc tggcctgctt
    25381 tcatcggcaa tcgccagccg gtccagacgg cgttatcatc caaccgcgct tcgcaacctg
    25441 cacccgtcag ggcaaaccac ccatcagttt cgaactcaac tgttaactga ccgagcgtga
    25501 tctccagcgc gggggcattg gcgtcattac ccaccagcag gttagcaatg cgcagcgcag
    25561 gcatatccag tgcgccacag tggctgatac ccgactggcg aaaaccgtga cgaccgccat
    25621 cctgcacagt ggtgtacatg cccgcacgaa taatcttcag catattccct ccttctgcgg
    25681 tacaaatcgc acgctgtctc ccggacgtaa taagatgggt tcgtcacgcg ccgaatcaaa
    25741 caggctgagt gaggtatgac caatcaactg ccagccaccc ggcgttgcca gcggataaac
    25801 accagtctgc ggcccgccga tcccgacaga acctgccgga acgagcaagc gcggttcagc
    25861 gcgccgtggc gtgtgtagtt gttccggcaa actcccgaga tacgggaagc ccggttgaaa
    25921 acctaaaaac cagaccacgt attccacgga ggagtgcaat tcaacaacct gtttttcgct
    25981 caacccgcaa tacgccgcga ccaccgccaa atccggtccg cctgcaccac cgtaaaccac
    26041 cggaatttca ataaagcgag actcaggttc cagggcctcg ctctcctccc accagcgttg
    26101 caaacgctct atggcatcca gcgccagcga ctcaggatta cgcaaaatca ccgtgatatt
    26161 gttcatgccg ggaatggctt caaccacatt cggcatatcc accagacgct gcgccagtcg
    26221 ccagatccgt ttctggctag ccagcgtcac cggcggttcc agttccagca ctaccgccgt
    26281 ttcacctatc agataacaac gcgctcgttg cactttcgat ccctctgatt attcgttatg
    26341 caggattagg aatatcaata aaggtcacat caagatcggt attttcattc agccactcgc
    26401 tcaatgcgcg aataccaccg cgttcagtgg catggtgacc tgcagcataa aaatgcaatc
    26461 cctgctcgcg ggctgaatga atggtctgtt cagaaacttc gccagtaata aaagcatcca
    26521 cgccaaaacg cgcggcgcta tcgataaaac tttgcccgcc gcccgtgcac caggcgacgc
    26581 gctggaccac ctcaggtccg gtatcgccac accataacgg cttacgtccc agacgcgctt
    26641 caatccagga ggctagttcc attcccggca caggcatggt cagctctccc cacggcacca
    26701 acggctcaat ttcgcccatg accgtgatcc ccagtaacgc cgccagctgt gcgttattgc
    26761 ccagctcagg atgtgcgtca agcggcaaat gccagccata caggttgata tcattcgcca
    26821 gcaacgtttt taaacggtta cgcttcattc cgcgaatgac cggagactct cctttccaga
    26881 agtagccatg atgcacaatg accgcatccg cgcccagacg cactgcctca tcgagcaaag
    26941 cctggctggc ggtgacaccg gtaacaattt tttgcaccgt ctctttgcct tccacctgca
    27001 aaccattcgg cgcgtaatca ctaatcgccg cgctgttcag tttttcgtta atcagttgtt
    27061 ccagttcggt gtttttcatc ttcgcgcctt ccgtattctg atagctacac cgttaacata
    27121 gcgttacctc gcctgttcgt cgatagccaa tcttttgaca tattaagaaa caaacccgac
    27181 gccaaaagac gcaaattgtt tatatcatca cgcttcagct aatgtatatt tatgcaatca
    27241 gacagcatat tgcataaata atcagatctg tatcaaagtt ttaactttaa ggaaacacaa
    27301 gcacgccgcg caactattac gtgacgttgc cgtcaattgt ggaattatta tgaataaaca
    27361 cgcatcacaa ccgcgcgcta tttactatgt cgttgcgctg caaatctggg aatattttag
    27421 cttttacggc atgcgtgccc tgctgattct ctatctcacc aatcaactaa aatacaacga
    27481 tactcacgcc tacgagttat ttagcgccta ctgttcgctg gtgtatgtca cgccaatcct
    27541 cggtggcttt ttggcggata aagttctcgg caatcgcatg gcggtgatgc tgggggcgtt
    27601 gttgatggcg atcggtcatg tggtgctggg tgccagtgag atccatccgt cattcctcta
    27661 tctgtccctg gcgattatcg tctgcggcta tggcctgttt aaatctaacg taagctgtct
    27721 gctcggcgag ctgtatgagc caaccgatcc gcgtcgtgat ggcggtttct cgctgatgta
    27781 tgcggcgggt aacgtggggt ctattatcgc acctatcgcc tgtggttacg cccaggaaga
    27841 gtacagctgg gcgatgggct ttggcctggc ggcggttggc atgatcgcgg gtctggtcat
    27901 tttcttatgt ggcaatcgtc atttcactca tacccgcggc gttaacaaaa aagtactgcg
    27961 tgcgacaaac tttctcctgc cgaactgggg atggctgctg gttctgctgg tggcaacgcc
    28021 tgcactgatt accgtgctgt tctggaaaga gtggtcggta tacgccttaa ttgtcgcgac
    28081 tattattggc ctgggtgtac tggcaaaaat ttatcgcaaa gcagaaaacc agaaacagcg
    28141 gaaggagctg gggctgattg tgacgctcac cttcttcagt atgttgttct gggccttcgc
    28201 acaacagggc ggtagctcga ttagccttta tatcgaccgc ttcgttaacc gcgatatgtt
    28261 tggttatacc gttccgaccg cgatgttcca gtcgattaat gccttcgcag ttatgctgtg
    28321 cggtgtgttc ctggcgtggg tggttaaaga aagcgtcgca ggtaatcgta ccgtgcgcat
    28381 ctggggaaaa tttgctcttg gccttggcct gatgagcgcc ggattctgca ttctgacctt
    28441 aagcgcccgc tggtccgcaa tgtatggtca ctcttctctg ccactgatgg tgttaggcct
    28501 cgcggtgatg ggctttgcgg aactgtttat cgacccggtt gccatgtcgc aaattacgcg
    28561 cattgaaatt ccgggcgtga ccggcgtatt aaccggcatc tacatgctgc tttctggcgc
    28621 gatcgcgaac tatctggcgg gcgtgattgc cgatcagaca tcgcaggctt cgtttgatgc
    28681 ttccggggcg atcaactact ccatcaatgc atatattgaa gtatttgatc aaattacctg
    28741 gggcgcactg gcgtgtgtag gactggtact gatgatttgg ctgtatcagg cgctgaaatt
    28801 cagaaaccgc gcgctggcgc tggagtcata ataggccagc accgatgcct gatgcgacgc
    28861 ttgacgcgtc ttatcaggcc tacgtatttc ctgcaattta ttgaatttgc acaaatttgt
    28921 aggctggatt aggcgttcac gccgcatccg gcatctgggc tcgattgcct gacgcgtctg
    28981 ttatttcccc ttccgcgccg cctcatacgc tgccaacgtt tgtattctcg cctctttgtg
    29041 ctcgactatc ggttgcggat aatccagctt cacacctgct ttctgcgccc acttccacgg
    29101 ctcatgcacc gctttccctg gtacatcgcg cagctccggt aaccatcgac gaataaactc
    29161 gccctcacgg tcaaatttct cgccctgagt tatcggattg aaaatacgaa aatacggcgc
    29221 agcatcagta cccgtagaag ccgcccactg ccagccaccg ttattagctg ccaaatcacc
    29281 atcaatcagc tgcgacatga aatatcgctc gccttcgcgc cagtcgatca acaaatcttt
    29341 caccagaaaa ctggctgtaa tcatccgtag ccggttatgc atccagccag tgctgttaag
    29401 ctgacgcata gcggcatcaa taatcgggta tcccgttttg cctttctgcc aggcctgtaa
    29461 atgtgcggga ttgctttgcc actgcacacg atccgtccag gcaataaatg gacgatgttt
    29521 acacaacgag gggtaatacg tcatcagatg acggtagaac tcgcgccaga tcagctcact
    29581 aagccagaca ctaccggccc caccgtccag cgcctgcggc tgttccgcca acaagcgatg
    29641 caaacactgg cgaggcgata acccgcccgt tgccaagctg gcggacaaac ggctggtgcc
    29701 ttccactgcc ggaaaatctc gttgttgctc atactctcca gcaccgttct cgcaaaactg
    29761 gcgtaattgc gcaatcgccg ctttttcttc caccgggaaa tgcgcagtat cgaaagactg
    29821 acgaggataa ttcagcgtaa tggatggcgc gggctttatc gatccgctac tacgaacttt
    29881 tggtgcagcg acgcactccg gcatcccttc ccgcagccgt ttcagccagg cattcttaaa
    29941 aggcgtaaag actttgtaca tctcgtgatt gccggtcatc accgcccccg gcggcaggat
    30001 cacgctgtca tcaaatcctt cacacaccac gttacgcagc gctctttcaa cgtccacatc
    30061 ccgcgctcgc tcattcacct catactgata gttataaaaa agatgagtaa cgctgttttc
    30121 cgcgcacacc tgtttaacta tttcgacact ggcgacaaag tcatccactt cacggaacaa
    30181 taaaggaata cctttttccg ccagcgcgat ttgtagccca ttcagttgag cattgataag
    30241 ttcagcctga cgcggcgaca tgttatgcgt ctcccactgg cgtggtgtag cgatatacag
    30301 cgccagcacg cgtgcagacg aattgcggca ggcggcagcc agtgcgagat tatcgtgcag
    30361 acgtaaatcc tggcgaaacc aggccagatg ggtagtcata aaactcctga atggcgcaag
    30421 ttaatcacac gcggccaaag ttcgaaataa cgctgccctg aaagccaggc gtcaggataa
    30481 agcgccatat agtgtttgat acgcatcagc ggcgcaagaa gtggttgctc gccacggcga
    30541 taactgtcga taagcgcagc cagctgctgg cgttctgtgg aatcaatatg cgggcgaaaa
    30601 taaccctgaa cgtgcatcaa aacgttggtg tgatcgcgcg ggttggcggg atgagaaagc
    30661 aaaacaatta cgcgttggcg gtactggttg taaaaatcat ccagattttg ccactgatga
    30721 atatcagcca caaacgggcc aagtttgcgg tactcgggct gagaatgggc cagaaagacg
    30781 agtttatagc ggctgtgata atcgagaagc gccccgcgcg ttaagccatg ttctttcagt
    30841 cgatgtaact catgaagggc gaagatacgg attagggtgc tgtcatcaaa tcgttgtaga
    30901 ttcattactg ctcctgaacg gtatacagga gtaagtgtag acgttggtaa atgatgtttg
    30961 tgaagttaat ccgctagcag ggcaagatta cgaaagcgcg ctccccgcaa gcaactaatc
    31021 aaggtagtta atcatggctg aacacgatat tacgccagaa gtcaccattt ccaaaacaca
    31081 gcgccgctat aaagtcggct atgtcagagc ccgccaccag gaccgcaaaa ccggtatagc
    31141 ccggtattat tcacaacatc ccagcctgca ccttaagggc gactggctga aagaggccgg
    31201 atttgatacc ggacggggtg tcacggtgaa gatttcggag gggtgcctga ccatcatcgc
    31261 tgacagtgac gaggtgcagg aactgcggga ggaactttat caggttaaac aggtagttaa
    31321 ggggatgcgg gatgggatgt ttagtgtgct gaacgaaagt taaatattaa aaaagctggg
    31381 aagatttaaa accttcctag ctttttattc tgtcttatta taatggatac tcatcatctt
    31441 ccgatgaatt tataaattgc tccaagttta catgaagagt acttaaccta gtttttaact
    31501 cattaagtga agcgataatt tcatattttc caacataatc atatgatg
//
LOCUS       DBKEKI010000037        30755 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.037, whole genome shotgun sequence.
ACCESSION   DBKEKI010000037 DBKEKI010000000
VERSION     DBKEKI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.037"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..578
                     /locus_tag="ACUQPX_001662"
     CDS             <1..578
                     /locus_tag="ACUQPX_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HHV9056402.1"
                     /translation="ERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVG
                     IKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKF
                     ESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTL
                     IHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            808..1191
                     /gene="secE"
                     /locus_tag="ACUQPX_001663"
     CDS             808..1191
                     /gene="secE"
                     /locus_tag="ACUQPX_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HHV9056403.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1193..1738
                     /gene="nusG"
                     /locus_tag="ACUQPX_001664"
     CDS             1193..1738
                     /gene="nusG"
                     /locus_tag="ACUQPX_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HHV9056404.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1897..2325
                     /gene="rplK"
                     /locus_tag="ACUQPX_001665"
     CDS             1897..2325
                     /gene="rplK"
                     /locus_tag="ACUQPX_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HHV9056405.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2329..3033
                     /gene="rplA"
                     /locus_tag="ACUQPX_001666"
     CDS             2329..3033
                     /gene="rplA"
                     /locus_tag="ACUQPX_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HHV9056406.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3446..3943
                     /gene="rplJ"
                     /locus_tag="ACUQPX_001667"
     CDS             3446..3943
                     /gene="rplJ"
                     /locus_tag="ACUQPX_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HHV9056407.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4010..4375
                     /gene="rplL"
                     /locus_tag="ACUQPX_001668"
     CDS             4010..4375
                     /gene="rplL"
                     /locus_tag="ACUQPX_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HHV9056408.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4695..8723
                     /gene="rpoB"
                     /locus_tag="ACUQPX_001669"
     CDS             4695..8723
                     /gene="rpoB"
                     /locus_tag="ACUQPX_001669"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HHV9056409.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLGLGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8800..13023
                     /gene="rpoC"
                     /locus_tag="ACUQPX_001670"
     CDS             8800..13023
                     /gene="rpoC"
                     /locus_tag="ACUQPX_001670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HHV9056410.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13265..14398)
                     /gene="thiH"
                     /locus_tag="ACUQPX_001671"
     CDS             complement(13265..14398)
                     /gene="thiH"
                     /locus_tag="ACUQPX_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HHV9056411.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14395..15165)
                     /gene="thiG"
                     /locus_tag="ACUQPX_001672"
     CDS             complement(14395..15165)
                     /gene="thiG"
                     /locus_tag="ACUQPX_001672"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HHV9056412.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15167..15367)
                     /gene="thiS"
                     /locus_tag="ACUQPX_001673"
     CDS             complement(15167..15367)
                     /gene="thiS"
                     /locus_tag="ACUQPX_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HHV9056413.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15351..16106)
                     /gene="thiF"
                     /locus_tag="ACUQPX_001674"
     CDS             complement(15351..16106)
                     /gene="thiF"
                     /locus_tag="ACUQPX_001674"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HHV9056414.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16099..16734)
                     /gene="thiE"
                     /locus_tag="ACUQPX_001675"
     CDS             complement(16099..16734)
                     /gene="thiE"
                     /locus_tag="ACUQPX_001675"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HHV9056415.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16734..18629)
                     /gene="thiC"
                     /locus_tag="ACUQPX_001676"
     CDS             complement(16734..18629)
                     /gene="thiC"
                     /locus_tag="ACUQPX_001676"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HHV9056416.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYSDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18710..18810)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18863..19339)
                     /gene="rsd"
                     /locus_tag="ACUQPX_001677"
     CDS             complement(18863..19339)
                     /gene="rsd"
                     /locus_tag="ACUQPX_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HHV9056417.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19434..20207
                     /gene="nudC"
                     /locus_tag="ACUQPX_001678"
     CDS             19434..20207
                     /gene="nudC"
                     /locus_tag="ACUQPX_001678"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HHV9056418.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20247..21311
                     /gene="hemE"
                     /locus_tag="ACUQPX_001679"
     CDS             20247..21311
                     /gene="hemE"
                     /locus_tag="ACUQPX_001679"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HHV9056419.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRCVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21468..22139
                     /gene="nfi"
                     /locus_tag="ACUQPX_001680"
     CDS             21468..22139
                     /gene="nfi"
                     /locus_tag="ACUQPX_001680"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HHV9056420.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22182..22772
                     /locus_tag="ACUQPX_001681"
     CDS             22182..22772
                     /locus_tag="ACUQPX_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HHV9056421.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22959..23231
                     /gene="hupA"
                     /locus_tag="ACUQPX_001682"
     CDS             22959..23231
                     /gene="hupA"
                     /locus_tag="ACUQPX_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HHV9056422.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23244..23939
                     /locus_tag="ACUQPX_001683"
     CDS             23244..23939
                     /locus_tag="ACUQPX_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HHV9056423.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23941..24366)
                     /gene="zraP"
                     /locus_tag="ACUQPX_001684"
     CDS             complement(23941..24366)
                     /gene="zraP"
                     /locus_tag="ACUQPX_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HHV9056424.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24604..25980
                     /gene="zraS"
                     /locus_tag="ACUQPX_001685"
     CDS             24604..25980
                     /gene="zraS"
                     /locus_tag="ACUQPX_001685"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HHV9056425.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     MRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25977..27302
                     /gene="zraR"
                     /locus_tag="ACUQPX_001686"
     CDS             25977..27302
                     /gene="zraR"
                     /locus_tag="ACUQPX_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HHV9056426.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCATLNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27299..28588)
                     /gene="purD"
                     /locus_tag="ACUQPX_001687"
     CDS             complement(27299..28588)
                     /gene="purD"
                     /locus_tag="ACUQPX_001687"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HHV9056427.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28600..30189)
                     /gene="purH"
                     /locus_tag="ACUQPX_001688"
     CDS             complement(28600..30189)
                     /gene="purH"
                     /locus_tag="ACUQPX_001688"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HHV9056428.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLTIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVSGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7218 a         8001 c         8459 g         7077 t
ORIGIN      
        1 cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc tccatctccg
       61 gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt ggtgaagaag
      121 ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt gaaatgttcc
      181 gcaaactgct ggacgaaggg cgtgctggtg agaacgtagg tgttctgctg cgtggtatca
      241 aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc aagccgcaca
      301 ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt catactccgt
      361 tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact ggtaccatcg
      421 aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg gttgttaccc
      481 tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa ggcggccgta
      541 ccgttggcgc gggcgttgtt gctaaagttc tgagctaatt gccgataaca tttgacgcaa
      601 tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc agaacctggc
      661 tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga gggcgtataa
      721 tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt
      781 tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca aggaagcggg
      841 cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc
      901 ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt agtaattctg
      961 attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac cgttgctttt
     1021 gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca ggaaacattg
     1081 cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct gtggggactg
     1141 gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg agatgtctga
     1201 agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc
     1261 aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg gtgaagtcat
     1321 ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa
     1381 attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct ggcacctggt
     1441 gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc ctgcgccaat
     1501 cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg ataagccgcg
     1561 tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga
     1621 cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag tgtctgtttc
     1681 tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa aagcctaacc
     1741 cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg aatacaattt
     1801 tgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca cggggagcct
     1861 ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt acaagcctat
     1921 gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg tccggctctg
     1981 ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa aactgattcc
     2041 atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg ttctttcact
     2101 ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct
     2161 ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa
     2221 atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat gactcgctcc
     2281 atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat ggctaaactg
     2341 accaagcgca tgcgtgttat ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac
     2401 gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag cgtggacgta
     2461 gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta
     2521 ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct
     2581 gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc tgaccagatc
     2641 aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt
     2701 ggccagctgg gccaggttct gggtccgcgc ggcctgatgc caaacccgaa agtgggtact
     2761 gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt tcgttaccgt
     2821 aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga cgctgacaaa
     2881 ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg
     2941 aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg tgttgcagtt
     3001 gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggagg tgattttgtc
     3061 tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat attcgggttg
     3121 tggcaaggcg gcaactgagt gagtcgccag gagcatagct aactatgtga ctggtgcgaa
     3181 tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat ttgttcgttg
     3241 gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga aacttaatcc
     3301 cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct tgtttctgct
     3361 caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag agattttctc
     3421 tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac aagcgattgt
     3481 tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg
     3541 cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat
     3601 gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct
     3661 gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc cgggcgctgc
     3721 tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc
     3781 ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc tgccgaccta
     3841 cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg gcaaactggt
     3901 tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt tatcttttta
     3961 acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa tgtctatcac
     4021 taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg tagaactgat
     4081 ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg tagctgctgg
     4141 cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag ctgctggcgc
     4201 taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc
     4261 taaagacttg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca aagacgacgc
     4321 agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta aataagccaa
     4381 cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta gtcaccagcc
     4441 tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct gtgcctttca
     4501 atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct gtgtaaatcg
     4561 caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc attttccggt
     4621 caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga cttgtcagcg
     4681 agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt aaggattttg
     4741 gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt gactcgtttc
     4801 agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct ttccgttccg
     4861 tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc taccgccttg
     4921 gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc
     4981 tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc accgtaaaag
     5041 acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac aacggtacct
     5101 ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct
     5161 tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat aacgcgcgca
     5221 tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac aacctgttcg
     5281 tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca
     5341 ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc cgtgataaca
     5401 agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg
     5461 aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc
     5521 agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc gcaggtaaag
     5581 tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca gcgaacatgg
     5641 agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt atcgaaacgc
     5701 tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt gtcgacccaa
     5761 ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc
     5821 cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa gaccgttatg
     5881 acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa gaaatcgaag
     5941 gttccggtat tctgagcaaa gacgacatca ttgatgttat gaaaaagctc atcgatatcc
     6001 gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt atccgttccg
     6061 ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag cgtgcggtga
     6121 aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg atcaacgcca
     6181 agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct cagtttatgg
     6241 accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca ctcggcccag
     6301 gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg actcactacg
     6361 gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc aactctctgt
     6421 ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt aaagtgaccg
     6481 acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc aactacgtta
     6541 tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac ctggtaactt
     6601 gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac atggacgtat
     6661 ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg
     6721 ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg
     6781 ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg
     6841 cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag
     6901 ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac ctgaccaaat
     6961 acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct ctgggtgaac
     7021 cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt gaactggcgc
     7081 ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc gaagactcca
     7141 tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac attcaggaac
     7201 tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga gatcaccgct gacatcccga
     7261 acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac attggtgcgg
     7321 aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa actcagctga
     7381 ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac gttaaagact
     7441 cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag gtctttactc
     7501 gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg
     7561 cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc agccgtatcc
     7621 gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc
     7681 gctggctggg gctgggcctg acagacgaag agaaacaaaa tcagctggaa cagctggctg
     7741 agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa
     7801 tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta tatctggcgg
     7861 ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa
     7921 tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg ccggtagaca
     7981 tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc
     8041 acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg aaacagcagc
     8101 aagaagttgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc gctgacgttc
     8161 gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg gctgaaaacc
     8221 tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta
     8281 aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg tacgatggtc
     8341 gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg ctgaaactga
     8401 accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc ctggttactc
     8461 agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag atggaagtgt
     8521 gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcact gttaagtctg
     8581 atgacgtgaa cggtcgtacc aagatgtata aaaacatcgt ggacggcaac catcagatgg
     8641 agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca
     8701 acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc ggggtaaaac
     8761 ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt attaaagttt
     8821 ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct ggcttcgcca
     8881 gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat caactaccgt
     8941 acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc ggtaaaagat
     9001 tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag
     9061 tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca catcgaactg
     9121 gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg
     9181 ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta tgtggttatc
     9241 gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca gtatctggac
     9301 gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc aatccaggct
     9361 ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa
     9421 accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct ggaagcgttc
     9481 gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt actgccgcca
     9541 gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga cctgaacgat
     9601 ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg
     9661 ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg
     9721 gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct gaaatctttg
     9781 gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt
     9841 gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt
     9901 ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa gctggaactg
     9961 cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc
    10021 gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa ccgtgcaccg
    10081 actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg taaagctatc
    10141 cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga ccagatggct
    10201 gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc
    10261 aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta
    10321 ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg catggtgctg
    10381 actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct gcatgcgcgc
    10441 gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc
    10501 agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc gaaaggtctg
    10561 ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc
    10621 tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat catgtacacc
    10681 ggcttcgcct atgcagcgcg ttctggtgca tctgttggca tcgatgacat ggtcatcccg
    10741 gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat tcaggagcag
    10801 ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga tatctgggct
    10861 gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga aaccgttatt
    10921 aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat gatggccgac
    10981 tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg
    11041 gcgaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg
    11101 aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc ggataccgca
    11161 ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg
    11221 gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc ggttatcgag
    11281 ggtggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac
    11341 gttctgaagc cgggtactgc tgatatcctc gttccgcgca acacgctgct gcacgaacag
    11401 tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc tgttgtatct
    11461 tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc gcgtggccac
    11521 atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt
    11581 acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa
    11641 tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg
    11701 aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact gatcgacgaa
    11761 ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc gaaaggcgat
    11821 ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac catgccggtt
    11881 atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca gaccattact
    11941 cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc cgcagaacgt
    12001 accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac
    12061 gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt
    12121 cagctggaag atggcgtaca gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa
    12181 tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct gttcgaagct
    12241 cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa
    12301 gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga tccgtacgaa
    12361 gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt
    12421 gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat
    12481 gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca gggcgttaag
    12541 attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt
    12601 aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg cgtcaagatc
    12661 gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg
    12721 ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc gttccaggag
    12781 accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg
    12841 aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc gtaccaccag
    12901 gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt gactgcagaa
    12961 gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc tgataacgag
    13021 taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt atgggtgtta
    13081 ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac gaggaagaag
    13141 cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca caacgctttc
    13201 aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt tcttacattc
    13261 cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc cataccggct
    13321 gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg
    13381 agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt ttcgagaagg
    13441 cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt gattcccgcg
    13501 tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta
    13561 actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg cgcaggcgcg
    13621 gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc aaatgttcgg
    13681 caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg ccgaggccta
    13741 tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag aagaagtcct
    13801 gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa
    13861 ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc gccagcggtt
    13921 gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga cgacgaaagt
    13981 aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt tcaaagccca
    14041 tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt ttgcgcttga
    14101 tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat
    14161 aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga cgctgcgcct
    14221 gttgggccag ttgttccaga tagccactgg cggcaggcga taacagcgcc atcatgtcgt
    14281 cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt ttgccgttga
    14341 tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg attgctgaag gttttcatgc
    14401 cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaataac tgcggctgcc
    14461 cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat
    14521 gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt cggcccccat
    14581 ttccagcgcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa ccaccggaac
    14641 tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct gattcgagcc
    14701 aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac gtttacacaa
    14761 taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca gcgtttcggc
    14821 ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa
    14881 ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc
    14941 cgctgttttc gccccggaag tattcggcag cagggccaca cccgccgcga taagcggttc
    15001 gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg
    15061 gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc
    15121 ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa
    15181 taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc cactgctcac
    15241 gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct
    15301 ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg ttaaacagga
    15361 tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc
    15421 cactggctcg acttaccgtc gaacagtcgg agttctcccg caggcgtctc tataccgctt
    15481 aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc
    15541 acgcccgccg tgcggcagtt gcgttctggc tgctggttat ctggccacag gcagcggtaa
    15601 cacccctgct cccagggcgg tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg
    15661 gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg gcgagtcgcc
    15721 atattgtcgg tacagtcgag caccacatcg gcttgtgcaa ccgcatcttt caacgcctca
    15781 cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc
    15841 tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag gatttgtcgt
    15901 tgcagattgc ttaaatgcac atcgtcgtca tcagccagta ccagcgtccc gacgccagcc
    15961 cctgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat cagcacctgg
    16021 ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta
    16081 taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca actgtgccgt
    16141 tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac tgccgacacc
    16201 cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca cggtgggata
    16261 atccgccagg cgctcaacat gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat
    16321 ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc gtgctgccag
    16381 cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg
    16441 gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc catacgcctg
    16501 atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat agcggcggcc
    16561 cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat ctttgatgcg
    16621 tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta cgctgtccac
    16681 caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat acatcacgct
    16741 tcctccttat gcaggtagat ttctccgcct ctggcacgga agttctccga catatccgcc
    16801 attcccactt caatggtttg cgcggcggcg taatcgcgta cttcctggct gattttcatc
    16861 gagcagaatt ttggcccaca catggagcaa aaatgggcga ctttgccgga ctcttggggc
    16921 agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt aaactggtct
    16981 tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg cgcgcccgga
    17041 tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat aagtgataag cccctgctta
    17101 acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca gagcatcgcg
    17161 cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc atagcccggt
    17221 gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca gtgctctaac
    17281 tcctcggtca tattgcggcg gatcatctgc atcggcacat ggcctgggcc ttcaatcatc
    17341 acctgtacgt catattccca ggcgattttg gtcagttcgc ccagcgtatg caattcggca
    17401 aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc gcccagcgac
    17461 agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg atagaggaaa
    17521 ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg agagacgata
    17581 ccggtcaggc gtttcgcggt catcggcaca tagcgcagca ggacgcccgc atggatagtg
    17641 aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta
    17701 agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac tgtaccgatc
    17761 ggcaccggac tgttacgcaa aatccactcg cgggtttcgt gaatatagcg accggtggag
    17821 agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc cacttcttct
    17881 tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac caggaagtta
    17941 cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat aatcgcacgt
    18001 ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag atgtgcgcca
    18061 aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc gcggcccata
    18121 ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc
    18181 agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc gctaaaacgt
    18241 agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact gcgcacggta
    18301 agttcttcgg tatcggcgcg cgcatcgatc cacggctggc gtagttttgc cagcccttgc
    18361 tgcacgttaa tggcgatctg cggatcacta tacgggccgg aggtgtcgta gaccggaatc
    18421 gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt cgggctaagc
    18481 tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt
    18541 gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt
    18601 tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag gacgtggctt
    18661 gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata gcagattaac
    18721 tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa taaacggtct
    18781 cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt tgaatgtaaa
    18841 ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg tcaagcacca
    18901 gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg atgtcagata
    18961 aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg ctggaatcgt
    19021 agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg
    19081 cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg aaatgtccgg
    19141 cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg tttagcctca
    19201 tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg agcagatgct
    19261 tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt
    19321 tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact gctcagtgag
    19381 aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga gctatggatc
    19441 gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa aaattatggt
    19501 tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg
    19561 cactacaaat cggcgaatgg cagggagaac ctgtttggtt aatacaacag cagcggcgtc
    19621 acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt caactggccg
    19681 gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt tactgcgggc
    19741 atgaaatgta tccgagcaaa accgaatggg cgatgctgtg cagccattgc cgtgagcgtt
    19801 actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat tcgatcctcc
    19861 tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga ttcgtcgaag
    19921 tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag
    19981 ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct ttaatgaccg
    20041 cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg
    20101 cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc
    20161 gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga tacactgacc
    20221 gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg
    20281 cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag gcgggtcgct
    20341 atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa
    20401 acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg
    20461 cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg ctctattttg
    20521 aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac gtcgataaac
    20581 tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg cgtaccattc
    20641 gtcgcgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg tggacgctgg
    20701 caacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt
    20761 atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc gtcactttgt
    20821 atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac acctggggcg
    20881 gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat aaaattgttg
    20941 atggtttact gcgtgaaaac gacggtcgct gcgtgccagt cacgctgttt accaaaggcg
    21001 gcggacagtg gctggaagcc atggctgaaa ccggttgcga tgcgctgggc ctcgactgga
    21061 caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg cagggtaata
    21121 tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta gcgactatac
    21181 ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc attcatcagg
    21241 atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt
    21301 atcaccgcta agagcctgat ccatcaggct attttatttg ccattttgag gccgggcagt
    21361 gctcaaaatc ctcacgtact acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa
    21421 ctgccttcaa caattacgcc tgttgaacca ggctctaagg agcgattatg gatctcgcgt
    21481 cattacgcgc tcaacaaatc gaactggctt cttctgtgat ctgcgaggat cgactcgata
    21541 aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc ggagaagtga
    21601 cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag tataaagttg
    21661 cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc gaatatcctg
    21721 cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc
    21781 atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggctta ttggtggatg
    21841 tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg ctctccagcg
    21901 aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg gtctggcgca
    21961 gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg
    22021 cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca acgcgctggg
    22081 ctgacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat
    22141 tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa aacccaattc
    22201 atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct tgcttatgcg
    22261 aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt gatgggcaaa
    22321 tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa
    22381 atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac gatttcgatc
    22441 tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc
    22501 gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc atcacgaccg
    22561 ttgcgatgct ggaaatgact caggctggtc gcgaaatgag cgatgaagag cttaaagaaa
    22621 acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt ttagctgagt
    22681 gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt gagtcgggtg
    22741 agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt aacgcctgat
    22801 ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa
    22861 aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc gtcgcactcg
    22921 atgcttagca agcgataaac acattgtaag gataacttat gaacaagact caactgattg
    22981 atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct ctggagtcca
    23041 ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg gttggtttcg
    23101 gtaccttcaa agtgaaccac cgcgctgagc gtactggccg caacccgcag accggtaaag
    23161 aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca ctgaaagacg
    23221 cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt tcaccccttt
    23281 tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact gcctgtagcc
    23341 acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag ggcgcggtac
    23401 gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt agcccgtggc
    23461 gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc acgctcatca
    23521 atatcaatgt ttacagcaaa ccgccggtga acattcgcgc gcgttttgac gatcgcggcg
    23581 atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct aacgaccaaa
    23641 tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc ttacgtcagg
    23701 ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg accacctgcg
    23761 aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc gagcgccgcc
    23821 agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat
    23881 tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa acgttctgat
    23941 ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc ccatgcccat
    24001 tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca cccgtaactc
    24061 atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt tgctgctatc
    24121 cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca gttgctgttg
    24181 cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg tctgttgttc
    24241 gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag caaatgcaga
    24301 tgtcgatccc atcgccattg ctgaaagcgc catcattacc aaggcaattt tcgtgttccg
    24361 tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc cgtgccaacg
    24421 atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg actcgcctgc
    24481 tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac agcgcagggt
    24541 aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca ggcaaagaag
    24601 aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag cgcgatcctc
    24661 cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg cgattatggg
    24721 cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact tattcgcgct
    24781 cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc
    24841 ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa
    24901 ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta ttcccctcag
    24961 gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga tagcgcagac
    25021 ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc tggaatgcac
    25081 cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat cgctttcgac
    25141 gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat tattctcttc
    25201 gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg ccgctatatg
    25261 cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt ggcgctgggg
    25321 catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat taaaggactg
    25381 gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg
    25441 atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agttgctgga actggttaag
    25501 ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca ctcattacag
    25561 ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc caacgacaca
    25621 ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa tctctatctc
    25681 aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag cgaaagcggc
    25741 gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa
    25801 gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg gctggcggtc
    25861 gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca ggagggaaaa
    25921 ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc acaaggatga
    25981 cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc actattttgc
    26041 aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg cgacaggcgc
    26101 tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga atggcggaga
    26161 tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt ccggtgctga
    26221 ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact ggggcgctgg
    26281 attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa aaggcgctcg
    26341 cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag ttcggtatgg
    26401 tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg
    26461 aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc gccagggcga
    26521 ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt gcgacactca
    26581 acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt actggagccg
    26641 ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa
    26701 ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag
    26761 ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt gcggcgaccc
    26821 atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc
    26881 tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat atccctctgc
    26941 tggctggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta aaaggcttta
    27001 cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt cgtgaactgg
    27061 aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc gaacgcgagc
    27121 tgccgctggc gattgccagt acgccgatcc cgctggggca aagtcaggat attcagccgc
    27181 tggtggaagt ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa
    27241 ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt
    27301 agttctgctc gcgttcgatg gcgcgccaac cgatatcttt ccggcagaag caatcatccc
    27361 agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca
    27421 gcgcggtgac gcacagtacg cgcccgccgc tggttaccac ctgctcgtca tccgccagtt
    27481 ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg ccgtggatca
    27541 catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg ctgagagaag
    27601 cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga
    27661 gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga tcgccaaagc
    27721 ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg cccgcataga
    27781 gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc cagatgatac
    27841 gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag taagcgccca
    27901 tcccgccggt gtttggtccg ttatctttat cgcctacgcg tttgtgatcc tggctggtag
    27961 ccatcggcag cacatgctcg ccgtccacca tcacgataaa gctcgcttct tcgccatcga
    28021 ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc gccagcatat
    28081 cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct ttcccggcag
    28141 ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac accagcgcag
    28201 gttctacctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg gccaggaaat
    28261 ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg ccgaagattt
    28321 tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct tccgggccga
    28381 cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg atatcggtca
    28441 cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta cccggagcaa
    28501 caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc
    28561 cgccgttacc aatcactaat actttcatct attgctccat taatggcgga agtggcgcat
    28621 gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg
    28681 gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg catcaatacc
    28741 gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt ccaggccttc
    28801 atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct ggcccgcgcc
    28861 aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca cgaatttcgc
    28921 cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac gttgggtgac
    28981 gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca gcagaccgcc
    29041 gttcacgcgt ttgaaatcaa gacccgaaac acgctcgccc cactgaccgc aggtcagaac
    29101 gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg aaggcgcaat
    29161 aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg
    29221 gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta
    29281 agcatcaaga atggaattgc cgatagctac accgcaaggg ttggcgtgct tcacaatcac
    29341 acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt
    29401 gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg cttctttcac
    29461 attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac gcatatcctg
    29521 cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc
    29581 accgtggtac gccggaacca tgctgccgaa gtagttggca atcatgctgt cgtaggcggc
    29641 agtgtgttcg aaggctttga tggtgaggtc gaaacgggtt gcaagcgtca gcgatccttc
    29701 gttgtcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca cgattgcgac
    29761 atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat cgatgttctc
    29821 aaccgcatct tccagcgagc agccttcacg agcaacagtc tgggcgaacg gatacaggtt
    29881 aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat cgtcctggcc
    29941 gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc gtccatccat
    30001 catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt tttctgctaa
    30061 cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc
    30121 gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga ctggacgacg
    30181 ttgttgcatg gtaaatcccc tgggtttgac tattacagag agcgttagct gaatttttcg
    30241 cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc attgtaacga
    30301 aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa ttttgacagt
    30361 ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc tgtgcgtaaa
    30421 aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc tgcaattttt
    30481 ctattgcggc ctgcggagaa ctccctataa tgcgccttca tcgacacggc ggatgtgaat
    30541 cacttcacac aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca gggttgactc
    30601 tgaaagagga aagcgtaata tacgccacct cgcgacagtg cgctaaagcg cgtcgcaact
    30661 gctctttaac aatttatcag acaatctgtg tgggcactcg aagatacgga ttcttaacgt
    30721 cgcaagacga gaaatgaata ccaagtctca agagt
//
LOCUS       DBKEKI010000038        30412 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.038, whole genome shotgun sequence.
ACCESSION   DBKEKI010000038 DBKEKI010000000
VERSION     DBKEKI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.038"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..795
                     /locus_tag="ACUQPX_001689"
     CDS             <1..795
                     /locus_tag="ACUQPX_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HHV9056429.1"
                     /translation="WLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1783
                     /locus_tag="ACUQPX_001690"
     CDS             806..1783
                     /locus_tag="ACUQPX_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9056430.1"
                     /translation="MKRAPLITGLLLISTSCAYASTLSSTNWQYACSCSAGKAVKLVY
                     MVSPVLTTTGHQTGYYKLNDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYS
                     AATQTGVCQGDTSRYGPVNIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGS
                     PSTGDFHDLVKLSIQGNLTAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVD
                     FDITYDCGDTSKIKNSLQMRIDGTTGVVDQYNLVARRRSSDNVPNVGIRIENLGGGVA
                     NIPFQNGILPVDPSGHGTVNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1826..2686)
                     /gene="rsmI"
                     /locus_tag="ACUQPX_001691"
     CDS             complement(1826..2686)
                     /gene="rsmI"
                     /locus_tag="ACUQPX_001691"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HHV9056431.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2751..4787
                     /gene="lpoA"
                     /locus_tag="ACUQPX_001692"
     CDS             2751..4787
                     /gene="lpoA"
                     /locus_tag="ACUQPX_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HHV9056432.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4745..5140
                     /locus_tag="ACUQPX_001693"
     CDS             4745..5140
                     /locus_tag="ACUQPX_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HHV9056433.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5160..5750
                     /gene="diaA"
                     /locus_tag="ACUQPX_001694"
     CDS             5160..5750
                     /gene="diaA"
                     /locus_tag="ACUQPX_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HHV9056434.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5760..6335
                     /gene="dolP"
                     /locus_tag="ACUQPX_001695"
     CDS             5760..6335
                     /gene="dolP"
                     /locus_tag="ACUQPX_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HHV9056435.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6449..7489)
                     /locus_tag="ACUQPX_001696"
     CDS             complement(6449..7489)
                     /locus_tag="ACUQPX_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HHV9056436.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVTMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7562..8197)
                     /locus_tag="ACUQPX_001697"
     CDS             complement(7562..8197)
                     /locus_tag="ACUQPX_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HHV9056437.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8325..8843
                     /gene="yhbO"
                     /locus_tag="ACUQPX_001698"
     CDS             8325..8843
                     /gene="yhbO"
                     /locus_tag="ACUQPX_001698"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HHV9056438.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLATSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8823..9266)
                     /locus_tag="ACUQPX_001699"
     CDS             complement(8823..9266)
                     /locus_tag="ACUQPX_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HHV9056439.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9317..9619
                     /gene="yhbQ"
                     /locus_tag="ACUQPX_001700"
     CDS             9317..9619
                     /gene="yhbQ"
                     /locus_tag="ACUQPX_001700"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HHV9056440.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9606..10109)
                     /locus_tag="ACUQPX_001701"
     CDS             complement(9606..10109)
                     /locus_tag="ACUQPX_001701"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHV9056441.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10103..10627)
                     /gene="ubiT"
                     /locus_tag="ACUQPX_001702"
     CDS             complement(10103..10627)
                     /gene="ubiT"
                     /locus_tag="ACUQPX_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110142 - ubiquinone biosynthesis complex
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone anaerobic biosynthesis accessory
                     factor UbiT"
                     /protein_id="HHV9056442.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10836..11831
                     /gene="ubiU"
                     /locus_tag="ACUQPX_001703"
     CDS             10836..11831
                     /gene="ubiU"
                     /locus_tag="ACUQPX_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone anaerobic biosynthesis protein UbiU"
                     /protein_id="HHV9056443.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDDYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11840..12706
                     /locus_tag="ACUQPX_001704"
     CDS             11840..12706
                     /locus_tag="ACUQPX_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HHV9056444.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLASSELGELKRYVENGEFLIEASDLGVVNMCAER
                     KLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRNQFEV
                     EVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLNGIQT
                     MSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANSDCNG
                     YWRRLAGLELQA"
     gene            12787..13794
                     /locus_tag="ACUQPX_001705"
     CDS             12787..13794
                     /locus_tag="ACUQPX_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HHV9056445.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13912..15156)
                     /gene="mtr"
                     /locus_tag="ACUQPX_001706"
     CDS             complement(13912..15156)
                     /gene="mtr"
                     /locus_tag="ACUQPX_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HHV9056446.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15310..17199)
                     /gene="deaD"
                     /locus_tag="ACUQPX_001707"
     CDS             complement(15310..17199)
                     /gene="deaD"
                     /locus_tag="ACUQPX_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HHV9056447.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17192..17272)
                     /gene="yrbN"
                     /locus_tag="ACUQPX_001708"
     CDS             complement(17192..17272)
                     /gene="yrbN"
                     /locus_tag="ACUQPX_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HHV9056448.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17379..18263)
                     /gene="nlpI"
                     /locus_tag="ACUQPX_001709"
     CDS             complement(17379..18263)
                     /gene="nlpI"
                     /locus_tag="ACUQPX_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HHV9056449.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18372..20507)
                     /gene="pnp"
                     /locus_tag="ACUQPX_001710"
     CDS             complement(18372..20507)
                     /gene="pnp"
                     /locus_tag="ACUQPX_001710"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HHV9056450.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGCV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20754..21023)
                     /gene="rpsO"
                     /locus_tag="ACUQPX_001711"
     CDS             complement(20754..21023)
                     /gene="rpsO"
                     /locus_tag="ACUQPX_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HHV9056451.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21172..22116)
                     /gene="truB"
                     /locus_tag="ACUQPX_001712"
     CDS             complement(21172..22116)
                     /gene="truB"
                     /locus_tag="ACUQPX_001712"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HHV9056452.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYACQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LCELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22116..22517)
                     /gene="rbfA"
                     /locus_tag="ACUQPX_001713"
     CDS             complement(22116..22517)
                     /gene="rbfA"
                     /locus_tag="ACUQPX_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HHV9056453.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22681..25353)
                     /gene="infB"
                     /locus_tag="ACUQPX_001714"
     CDS             complement(22681..25353)
                     /gene="infB"
                     /locus_tag="ACUQPX_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HHV9056454.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25378..26865)
                     /gene="nusA"
                     /locus_tag="ACUQPX_001715"
     CDS             complement(25378..26865)
                     /gene="nusA"
                     /locus_tag="ACUQPX_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HHV9056455.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26893..27345)
                     /gene="rimP"
                     /locus_tag="ACUQPX_001716"
     CDS             complement(26893..27345)
                     /gene="rimP"
                     /locus_tag="ACUQPX_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HHV9056456.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27552..27628)
                     /locus_tag="ACUQPX_001717"
     tRNA            complement(27552..27628)
                     /locus_tag="ACUQPX_001717"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27592..27594),aa:Met,seq:cat)
     gene            27976..29319
                     /gene="argG"
                     /locus_tag="ACUQPX_001718"
     CDS             27976..29319
                     /gene="argG"
                     /locus_tag="ACUQPX_001718"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HHV9056457.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(29327..>30412)
                     /locus_tag="ACUQPX_001719"
     CDS             complement(29327..>30412)
                     /locus_tag="ACUQPX_001719"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HHV9056458.1"
                     /translation="FATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT         7317 a         8303 c         7606 g         7186 t
ORIGIN      
        1 tggttaccgc gtagccgggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcaac gctaagtagc actaactggc aatacgcctg
      901 ttcctgctct gcgggtaagg cagttaaact tgtctatatg gtcagccccg tacttaccac
      961 cactggacat cagacaggat attacaaact caatgacagc ctggatatta aaaccacatt
     1021 acaggcaaac gacattccag gactcacaac cgaccaggtt gtctctgtta acacccgatt
     1081 cacacagata aaaagcagca ccgtatattc tgctgcaacc caaacgggtg tttgccaggg
     1141 tgacacgtct cgttatggac ccgttaatat tggtgcaaat accaccttta ccctgtatgt
     1201 caccaagcca tttctcggct cgatgaccat tccgaaaacg gatattgccg tcattaaagg
     1261 cgcgtgggtc gatggaatgg gaagcccgtc tacaggtgac ttccatgatt tagtcaagtt
     1321 atcgattcag ggaaatctca ccgccccaca gtcgtgcaaa attaatcagg gcgatgttat
     1381 taaagttaat tttggattca tcaatggtca gaagtttacc acccgcaatg ccatgccaga
     1441 cggttttact ccagtagact ttgatatcac ttatgactgt ggtgatactt caaagattaa
     1501 aaactcgttg caaatgcgca ttgacggtac aactggggta gtagaccagt acaacctggt
     1561 cgccaggcga agaagttcag acaatgtgcc caatgtcggt attcgtattg aaaatctcgg
     1621 cggcggagtt gcaaatattc cttttcagaa cggtatcctt cccgttgatc cttccgggca
     1681 tggcaccgtc aatatgcgcg cctggccagt taatctggtc ggtggtgagc tggaaacagg
     1741 aaaatttcag ggcacagcca ccattaccgt catggtgcgg taaaagaaga cgttgcgttt
     1801 tgattaacgc aacgcttcgg cactgttacc cctgctgctc cagcgcatac ttatacagcg
     1861 catttttctt cacgccatga atttctgcgg ccagcgccgc cgcttttttc agcggcagtt
     1921 ctgcctgtag cagcgccagc gtgcgcaggg catcggcggg taagtcttct tcctgtgctt
     1981 tatgaccttc gacaatcagc accatctcgc ctttgcgacg gttttcatct tcctttaccc
     2041 acgccagcag ctcgccaacg ggcgcgccgt gaatggtttc ccaggttttg gtcagctcac
     2101 gcgccagaac cacgtagcgg gattcgccta ataccgcaac gatatcttcc aggctatcta
     2161 acagacggtg ggtagattca taaaaaatca gcgtgcgcgg ctccgcttca atggctttta
     2221 gcgcatcacg gcggcctttt gatttggcag gtaaaaagcc ttcgtaacag aaacggtcag
     2281 atggtaaacc cgcggcgctt aacgcagtga tagcagcaca cggtccgggt agcggcacca
     2341 cacgaatccc cgcttcacgg caggtacgca ccagatggta gccaggatcg ttaattagcg
     2401 gcgttccggc atcggaaacc agcgcaatgt tttgcccctc ttgcagcttc gccagcagcg
     2461 tttcggcttt ttgttgttcg ttatggtcgt gcagcgcaaa caaccgggca ttaatcccaa
     2521 aatgttgcag caataaaccg gtgtgacgag tatcctcggc ggcaatcaga tcaacggcct
     2581 gtaatacctc taacgtacgc tgggtgatat ccgccagatt gccgattggc gtcggtacaa
     2641 tgtaaagctg gccctgagaa ttatccgccg attggtgttg tttcattgtg tcgtccgtat
     2701 tgccgattta atattgagca ttgcgtaaaa aaaatatcac tggatacatt atggtaccct
     2761 caacattttc tcgtttgaaa gccgcgcgtt gtctgcctgt tgttctggca gccctgattt
     2821 tcgccggttg tggcacccat actcccgatc agtccactgc ttatatgcag ggcacggcgc
     2881 aggctgattc tgccttttat cttcagcaga tgcagcaaag ctctgatgat accaggatca
     2941 actggcaatt actcgccatt cgtgcactgg tgaaagaagg taaaaccggg caggcggttg
     3001 agttgtttaa ccaactaccg caagaactga acgattctca gcgtcgcgag aaaacactgc
     3061 tggcggcaga gattaaactg gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga
     3121 aaatcacacc tgccgattta gaacaaaacc agcaagcgcg ttactggcag gcaaaaatcg
     3181 atgccagcca ggggcgtcct tccattgatt tactgcgcgc gttaattgct caggaaccgc
     3241 tgcttggcgc gaaagaaaaa cagcagaata ttgatgccac ctggcaggcg ctctcctcca
     3301 tgactcagga acaggcgaat acgctggtga tcaacgccga cgaaaatatt ctgcaaggct
     3361 ggctggatct gcagcgcgtc tggtttgata accgtaacga tcccgacatg atgaaagccg
     3421 ggatcgccga ctggcagaaa cgttatccga acaatccggg cgcgaaaatg ctgccaacgc
     3481 agttggttaa cgtaaaagcg tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc
     3541 cgctgaatgg ccaggcagcg gtatttggtc gcactattca gcaaggcttt gaagccgcga
     3601 aaaatatcgg cactcagcca gtggcggctc aggtagctgc cgcacctgcc gcagacgtag
     3661 cagaacaacc tcagccgcaa actgcggatg gcgttgccag cccggcacaa gcctcggtta
     3721 gcgatctgac cggtgatcag cctgcggccc agccggtgcc tgtaagcgcc ccggcgacaa
     3781 gcaccgcagc ggtaagcgca cccgcaaatc catccgcaga gctgaaaatc tacgatacct
     3841 catcacaacc acttagccag atcttaagcc aggttcagca ggatggcgcg agtattgtgg
     3901 ttggtccgtt gctgaaaaat aacgttgaag agttgctgaa gagcaacacc ccgctgaacg
     3961 tactggcact gaaccagccg gagaatatcg aaaaccgcgt caatatttgt tacttcgcgc
     4021 tttcaccgga agacgaagcg cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag
     4081 cgccgctggt gctgatccca cgcagtgcat tgggcgatcg cgtagccaat gcgtttgcgc
     4141 aagagtggca gaaactgggc ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg
     4201 aattacgcgc gggtgttaac ggcggttctg gtattgcttt aacgggtagc ccgattactc
     4261 ccagagcgac aaccgactcc ggcatgacga ccaacaatcc aacgctgcaa accacgccaa
     4321 ccgatgacca gttcaccaat aatggcggtc gtgtcgatgc ggtctacatt gtggcaacgc
     4381 cgggtgaaat cgcttttatc aaaccgatga tcgccatgcg taacggtagc cagagcggtg
     4441 caacgctgta cgccagttcc cgcagcgcac aaggcaccgc aggcccggat ttccgactgg
     4501 agatggaagg cttgcagtac agcgaaatcc cgatgctggc aggcggtaat ctgccgttaa
     4561 tgcagcaggc gctcagcgcg gtgaacaacg attattcgct ggctcgcatg tatgcgatgg
     4621 gcgttgatgc ctggtcgttg gcaaatcatt tctcacaaat gcgccaggtt cagggttttg
     4681 aaatcaacgg taataccgga agtctgacgg ctaacccgga ttgcgtgatt aacaggaagt
     4741 tatcatggct acagtaccaa caaggtcagg tagtccccgc cagttaacca ccaaacagac
     4801 cggcgatgcg tgggaagcac aagcgcgtcg ctggctggaa ggcaaaggac tgcggtttat
     4861 cgccgctaac gtgaacgagc gtggcggcga gatcgatctg ataatgcgtg aaggcctgac
     4921 caccgttttt gtcgaggtac gctatcgccg ctctgcgctt tatggcggcg cggcagccag
     4981 tgtgacccgc agcaaacaac acaaattatt acagactgcc cgcttgtggc tcgcgcgtca
     5041 taatgggagt tttgatactg tggattgccg gttcgatgtg gtagccttca ccgggaatga
     5101 ggttgagtgg attaaggatg cctttaatga ccactcataa ttaaggttta aggattagcg
     5161 tgcaagaaag aattaaagct tgcttcactg aaagcattca aactcaaatt gcggcggcag
     5221 aggcgcttcc ggatgccatc tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg
     5281 gcaacaaaat cctctgttgt ggtaatggaa cttccgctgc caatgcacag cattttgctg
     5341 ccagcatgat caaccgtttc gaaacggagc ggcccagctt acctgccatt gcactaaata
     5401 ctgataatgt tgtcttaacg gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa
     5461 aacaggtgcg ggcgctgggt catgcgggag atgtattgtt agccatttcc acccgtggca
     5521 acagccgcga tattgttaaa gcagttgaag ccgccgttac gcgtgatatg accattgtgg
     5581 cattgaccgg ctatgacggc ggcgaacttg caggtttgtt agggccacag gatgtggaga
     5641 tccgcattcc ttcgcatcgt agtgctcgca ttcaggaaat gcatatgctg acggtaaatt
     5701 gcctgtgcga tctgatcgat aacacgcttt tccctcacca ggatgattaa ggagaataca
     5761 tgaaggcatt atcgccaatc gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg
     5821 ccgctgccgt agtgggtacc gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg
     5881 tcggcaccca ggtggacgat ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag
     5941 acgaacagat taagaaagaa acccgtatta atgtcacggc ctatcagggc aaagtgctgc
     6001 tggttgggca gtcaccaaat gctgaacttt cggctcgcgc caaacagatt gctatgggcg
     6061 tagacggtgc caacgaagtg tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg
     6121 aagcatctaa cgatacgtgg atcaccacca aagtgcgttc gcagctctta accagcgacc
     6181 tggtgaaatc gtccaacgtg aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc
     6241 tggtgactga acgtgaagcg aaagcggcgg cagatattgc cagccgggtg agcggcgtaa
     6301 agcgggtaac cacagcgttt acgtttatta aatagcagcc ccttgtaatg cctgatgcga
     6361 cgcttgccgc gtcttatcag gcctacaacg atgcaaaccg taggtcggat aaggcgttta
     6421 cgccgcatcc gacatgtttt ccctcaaatc agaacaacag cgccagcccg ccgacaatca
     6481 caccagacac tgccaccatc gcccctgtca gccataaggc tttcgccggg aacgctttgc
     6541 gcagcataat cagtgacggc aaactcactg ccgggagcgt catcaacaat gccagcgccg
     6601 gagcggttcc catacctgcc agcatcatcg tttgtacaat cggaatttct gctgccgtgg
     6661 gaatgacaaa caagcatcct gctaccgcca tcgtcaccac ccacatcagg ctgttatcga
     6721 cagcaccatc ggcatggggg aataaccaga cgcgtgcggc acccaacacc agtactgcaa
     6781 ggatgtaaac cgggatcgta ctccagaaaa gtgtccatag cgccctgccc cagcggctaa
     6841 aaaaaccgct ctgtgcttcc ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt
     6901 cacgcaccca tttttgcacc agcgtcgcaa tcagcaacac catcaccagc ccggccacca
     6961 gacgaatcgc cgcaaaaccc cagccgagga caaaccccat aaacaccagc gtcgccgggt
     7021 ttaacaccgg attgcccatc cagaatgcca gcgcaccgcc catcgatacc tgttggcgac
     7081 gcattcccgc cgcgaccgga gccgcacagc aggtacacat catgcccggc agcgaaaaca
     7141 gcgttcccag cagcgtgccg cgaaagcgcg attgcccaag cgtacgcaac aaccagtcac
     7201 gcgggatcag cacctgaatc aacgaaccga ggatcacccc cagcaccgcc gctttccata
     7261 ctgcgaggaa atagatcatc gcgtaatcca acgccgcttg ccacggatta gcatccgcct
     7321 gcgcaaggat agatttaccg atactgtggg tttcggctgc agtaaacgct ttgccgtagt
     7381 aaggttgcca tttcacatac cagagaccgg caatgacaac gagaaagaaa agcgcgggtt
     7441 tccaccactg aatgggcgtt gccgcctgag atgaagactg accagtcata gcattcccca
     7501 ggaataagtt atgataattg agcgcgtgaa tattacgctc actatcaatt cttgggaata
     7561 attattcagc tcttttgcgt aattctgaag agcttaaaat cgtcacgcct tcatgctcag
     7621 gccgcatcgc ctctgccagc atgacccgcg ccacgtctct cgcatcaatg gatttccagt
     7681 tacctggtaa caaacggaac agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac
     7741 gatcgcccag taacatcgac gggcgagcaa tggtcaattt cggccagttc tgggcaatta
     7801 atgcttcttc catctcccct ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac
     7861 ccatagcact gaccaccagc atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat
     7921 ccactaccag cgtgtaatcg gcatgaataa acgcctcttt gctccccgct tctcgccgcg
     7981 tggtgccgag acaacaaaac acaatgtcga tgggttcggt gacctgcgcc aacgcgtcac
     8041 tcagttgcgg atcatgggga ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg
     8101 gcgcagcaat ggcgttaact ttcggttcat taatcaacat ccgcagcagg tgaccgccca
     8161 ccagccccgt tgcgcctgta atcagtacct gactcatctt cgctccttta cagaattgtc
     8221 tgccttgcgc tccccggctc atgcaccagt cttaatagac catgaggtaa ttatccccga
     8281 ttgtggaaaa ttcgcctcat ccaatgcaac aacgcggagg aagcatgagt aagaaaattg
     8341 ccgttttaat cactgatgaa tttgaagatt cagaatttac ttcacccgca gacgagttcc
     8401 gtaaagccgg acacgaagtg attactattg aaaaacaagc gggtaaaacg gtgaaaggca
     8461 aaaaaggaga agccagcgtg accatcgata aatccatcga tgaagtgacg ccagcggagt
     8521 ttgatgccct gttgctaccg ggtggtcatt caccggatta tctgcgtggc gacaaccgtt
     8581 ttgtcacctt tacccgtgat tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg
     8641 gtccgcagct gctgatcagc gccgatgtga ttcgggggcg caaactgacc gcagttaaac
     8701 cgatcattat tgatgttaaa aatgcgggcg tggaatttta cgatcaggaa gtcgtggttg
     8761 ataaagatca gctggctacc agccggacac cggacgatct gccagcgttt aaccgcgagg
     8821 cgttacgcct gctcggtgcc tgagtcgcgt agccaaatca tttttttacc aaagcccagc
     8881 gtgttgtcgg tgaatttgat ttcatcgagc cggatttccc acaccggggc cgacagcatt
     8941 ctggcaaccg gaaagcgacg attgtacgcc ttgcgcgcga ggtcgctttc ctcaccttcc
     9001 agcctgcgga tctcaccttt aaactgcaca ccgcgaatta acgctaccgt tttcggctga
     9061 ccgtttaccg ttccggcaac cgcagcctgc ggcccactca tctgcgcgtg gcgcgttttt
     9121 tcttccgtca aaatgtagaa ggcgactttc tgcgcatcaa aaagataaaa ggcattagcg
     9181 caccagagtt ccccttcctg ctgcacacac caggtgacaa catgttgttt tgccagccaa
     9241 cggctgatgg cgatgagtgt ttccattgct gttctctttt atactgtggg cctgacctta
     9301 acatgcgatg cagacgatga caccctggtt tctttacttg atccgtaccg ccgacaataa
     9361 gctttatacc gggatcacca cggatgtcga acgccgctat cagcagcacc aaagcggcaa
     9421 aggagcgaaa gcactgcgcg ggaaaggaga actgacgctg gcgttttctg cgccagtggg
     9481 cgaccgttcg ctggcactac gggcggaata tcgcgttaag caactgacca aacggcaaaa
     9541 agagcgtctg gtagcggaag gcgcagggtt tgcagaactg ttgagcagcc tgcaaacccc
     9601 ggagattaaa agcgattgaa atgctcgtga tactcaacca gaccggttac gccattcagc
     9661 gcgtcatccg ctaaacgatg tacctggaag gcgctttcag tgcccggcca gcggcaacgc
     9721 agatcgtgat gcgccgccag ttcaaagccg aaacgactgt acagcgccgg atcgcccagc
     9781 gtcaccactg cggcatagcc gaactcatta agcgaatcga gtccttcata gaccagttgg
     9841 cgtgccagcc cctgtccacg gtatttttca tcaaccgcca acggtgccat gccgacccat
     9901 tgcaggtctt cgccctgcac atcaaccgga ctaaatgcca catagccaat gacctgacct
     9961 tcgtcatctg tcgccaccag ccccagcgtc agaaagccat cttcacgcag atcgtgaacc
    10021 agcttcgctt ccgcatcact ttcgaatgag cgacgcagca gggcatcaat acccggcgca
    10081 tcaatgggaa tttctactcg aattagcatg gttcacctac cgatgtctgt ttggtttcag
    10141 gcgcggtttt cattcccgcc tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg
    10201 ctttcggcat ttgctccagt tcaatggcgt ccatcaggtt tttcacatac acccccagct
    10261 ccgtatcgcc ttcaatcacc agccgacgct ggaagaagag cgtatccgga tcttgtttac
    10321 gcgccgcaat catcagcaga tcgctggcgt cggcactaaa actcacatca gcttgcgcgt
    10381 tctggctgac caccagtttg ccattcacca ccgaggtaaa ccattgcagg tcaatatcac
    10441 gcacatgaat acttaaccag cggccttcaa gaaactccag ctcgccatca tccagcgcct
    10501 gacggaattg ccagcttaag acctgctcaa gaacctggcg ttttagcgca aatggcgtca
    10561 gttttaccgg tacactcaac agagatggcc ccaaatgcac aatacgggaa cgcagtttat
    10621 ccaacacgag ttttactccc tgtttcaaca atcatcctat tttgccatat cagaaaaata
    10681 acatagcggt ataaatcaac aattccatat gaaattgctg ctaccaccaa tacaacttta
    10741 actgccttaa atcaaaaatt gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt
    10801 tttacgtttt gatagcgcaa ccttcaggaa aaattatgga gctgctctgc cctgccggaa
    10861 atctcccggc gcttaaggcg gccatcgaaa acggcgcaga tgctgtttat atcgggctaa
    10921 aagatgatac caatgcccgt cacttcgccg gccttaactt taccgagaaa aaattgcagg
    10981 aagcggtgag ttttgtccat caacatcgcc gcaaacttca catcgcgatt aacacttttg
    11041 cgcatccgga cgattacgcc cgttggcagc gtgccgtgga tatggcggcg cagctgggtg
    11101 ccgacgcgct gatcctcgcc gacctcgcca tgctggagta cgccgccgag cgttacccgc
    11161 atattgaacg tcatgtgtca gtgcaggctt cggcgaccaa tgaagaggcg attaactttt
    11221 atcatcgcca ttttgacgtt gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg
    11281 tgaaacagct ggcacgggtt actcctgtcc cgctggaagt gtttgctttc ggcagcctgt
    11341 gcattatgtc ggaaggccgt tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca
    11401 ctgtgggtgc gtgttctccg gcccgtttcg tgcgctggca acaaacgccg caggggctgg
    11461 aatcccgcct gaacgaagtg ctgatcgacc gttatcagga cggcgaaaac gcaggttatc
    11521 cgacgctgtg taaagggcgt tatctggtgg acggcgagcg ctatcacgcg ctggaagaac
    11581 caaccagtct caataccctg gaactgctgc cggagttaat ggctgcgaat attgcttcgg
    11641 tgaaaattga aggccgccag cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc
    11701 gtcaggctat cgaccgttgt aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga
    11761 tggagacgct cgggtcgatg tccgaaggca cgcaaaccac ccttggcgcg tatcaccgta
    11821 aatggcagtg agaagagcaa tgaaatattc cttagggcca gtgctgtggt actggccaaa
    11881 agagacgctg gaagattttt atcagcaggc cgccgccagc agcgccgacg tgatttatct
    11941 tggtgaagcg gtatgcagca agcgccgggc aaccaaagtt ggcgactgtc tggagatggc
    12001 aaaatcgctc gccggaagtg gcaagcagat tgtgctctcc acgctggcat cctctgaact
    12061 gggcgaactg aaacgctatg ttgagaacgg tgagtttctg attgaagcca gcgatctcgg
    12121 cgtggtgaat atgtgcgctg aacgcaaact gccgttcgtc gccgggcacg cgctgaactg
    12181 ctacaacgcg gtgacgctga aaatattgct caaacagggc atgatgcgct ggtgtatgcc
    12241 ggtggagctt tcccgcgact ggctggtgaa tctgcttaat cagtgcgatg agctgggcat
    12301 tcgcaaccag tttgaagtgg aagtcctgag ctacggtcat ctaccgctgg cctactccgc
    12361 ccgctgcttt accgcgcgtt cggaagaccg cccgaaagat gagtgtgaaa cctgctgcat
    12421 taagtatccg aacgggcgca acgtgctgtc gcaggaaaac caacaagtgt ttgtactcaa
    12481 tggcattcag accatgagcg gctacgttta caacctcggt aacgagctgg catccatgca
    12541 gggattggtt gatgtagtcc gcctgtcacc gcagggcact gacactttcg cgatgctcga
    12601 cgccttccgc gctaatgaaa atggcgctgt gccactgccg ttgacggcaa acagcgactg
    12661 taacggctac tggcggcggc tggcaggact ggagctgcaa gcctaagtaa atagctcact
    12721 ttgttaacaa ctttaactat tctttaatgc agtattaaag attaatcggt aacaaagtga
    12781 gctgttatga ctgataaaac cattgcgttt tcgctactcg atctggcccc catacccgaa
    12841 ggctcttcag cgcgggaagc gttctcccac tctctcgatc tcgcccgtct ggctgaaaag
    12901 cgcggctatc atcgctactg gctggcagaa caccacaata tgaccggcat tgccagtgct
    12961 gcaacatcgg tattgattgg ctatctggcg gcgaatacca ccacgctgca tctggggtct
    13021 ggcggcgtga tgttgcctaa ccactcaccg ttggtcattg ccgaacagtt cggcacactc
    13081 aatacgctct atccggggcg aatcgatttg gggctgggtc gtgctccggg tagtgaccaa
    13141 cggacaatga tggcgctacg tcgtcatatg agcggcgata ttgataattt cccccgcgat
    13201 gtcgcggagc tggtggactg gtttgacgcc cgcgatccca atccgcatgt gcgcccggta
    13261 ccaggctatg gcgagaaaat ccccgtgtgg ttgttaggct ccagccttta cagtgcacaa
    13321 ctggcggcgc agcttggtct gccatttgcg tttgcctcac acttcgcgcc ggatatgttg
    13381 ttccaggcgc tgcatcttta tcgcagtaac ttcaaaccgt cggctcggct ggaaaaacca
    13441 tacgcgatgg tgtgcatcaa tattatcgcc gccgacagca accgcgacgc tgaatttctg
    13501 tttacctcaa tgcagcaagc ctttgtgaag ctgcgccgtg gcgaaaccgg gcaactgccg
    13561 ccgccgattc aaaatatgga tcagttctgg tcaccgtctg agcagtatgg cgtgcagcag
    13621 gcgctgagta tgtcgttggt aggcgataaa gcgaaagtgc gtcatggttt gcagtcgatc
    13681 ctgcgcgaaa ccgacgctga tgagattatg gttaacgggc agattttcga ccaccaggcg
    13741 cgcctgcatt cgtttgagct ggcgatggat gttaaggaag agttgttggg atagtgtgtc
    13801 ttaacgcggg aagccttatc cgagctggca acgctgtcct acgtaggcct gataagcgaa
    13861 gcgcatcagg cattgtgtag gcagcagaaa tgtcggataa ggcaccgctg attactgata
    13921 caccggcagt aaattaaagc tcgataaaat atgcaccagt gcgttgccga cgccaaacac
    13981 cagaatcagc gcaatcatcg gcttgccacc ccagacgcgg aatttcgggc tgccaaagcg
    14041 tttacgcgat gcgcgggcca gcagtgccgg aacaattgcc gcccagatgg tagccgctaa
    14101 accagcataa ccaatggcat acaggaatcc gttcgggaac agcagaccac ccacaacagg
    14161 cggggcaaag gtcagcaatg ccgttttcaa gcggcccaca gccgagtcgt cgaaaccaaa
    14221 cagatctgcc agatagtcaa acaaacccag cgttacgcca aggaacgaac tcgctaccgc
    14281 aaagtttgag aacacgacca gcagaaggtc caggctacgg ctgttcagca cgccgcttaa
    14341 tgcttgtacc agcacatcaa tattaccgcc cttctcggcg atgccgataa actccggacg
    14401 agggatgttg cccattgtcg ccagcaacca gatggtatac agcgccagtg ccatcagcgt
    14461 accgtacacc aggcatttca cgatggtttt cggatctttg ccgtaatact tcatcaggct
    14521 tggcacgtta ccgtgataac caaacgacgc caggcagaac ggcagggtca tcagcaaata
    14581 cggcgcataa gatgcattgc tttcggcaac gttgaacaat gtcgcaggct gcacatgccc
    14641 cagcaggcta ccgaaggtga ggaagaaggt aatgactttc gcccccagca caatcgccgt
    14701 catgcggctg acggctttag tactcaacca caccacaaac gctaccagca gtgcaaaacc
    14761 aaaacccgcc gcccgtgccg ggacgtttag cgacatctct gcgaaggtgt gatgcagaat
    14821 cgaaccactg gcagaaatat aggcataggt caggatatag agcacaaagg caatggaaat
    14881 gccgttgacc acgttccagc ctttgcccag caaatctttg gtgatggtgt caaaactcga
    14941 accgattcgg taattcaggt tagcttccag aatcatcaag ccggaatgca gcatacagaa
    15001 ccaggtaaag atcagcgctg ccattgacca gaaaaaccac gccccggaca tgaccactgg
    15061 cagagaaaac atccctgcgc caataatggt gccgccgata atcaccacgc cgccaagcag
    15121 cgacggtgac gtttgggtgg tggttagtgt tgccatgagg gcttctctcc agtgaaaaat
    15181 agtgcgactg cgttgttatg cattgcactg taccagtaca tgagtacaaa agacagaaaa
    15241 aaagccccga tggttaaaat cggggctgta tatattattt tacagattgt gttcgctgtt
    15301 cagcgatgat tacgcatcac caccgaaacg acgacgaccg gtagagtcat cacgacgcgg
    15361 tgcgcggcct tcacgacgtt cgccgctaaa acgacgacca tcaccacgac caccttcacg
    15421 gcgttcaccg ctgaagttac gaccgccttc acgacgctcg ccaccgaaac cacgaccacc
    15481 gccacgacgc tcaccgccag tatgcggctg tgcatcgccc agtaactgca tgttcatcgg
    15541 cttgttgaga atgcgagtgc gggtaaagtg ttgcagcact tcacccggca tacctttcgg
    15601 cagttcgatg gtggagtgag aagcaaacag cttgatgtta ccgatgtaac ggctgctgat
    15661 gtcgccttcg ttagcaatcg cgccaacgat atgacgaact tcaacaccat catcgcggcc
    15721 cacttcaatg cggtacagct gcatatcgcc aacatcacga cgttcacgac gcggacgatc
    15781 ttcacggtcg ccacgcgggc cacggtcgtt acgatcgcgc ggaccacggt catcacggtc
    15841 acggaattca cgtttcggac gcataggcgc atctggcgga acaatcagag tacgttcacc
    15901 ctgtgccatt ttcagcagtg ccgcagccag agtttcgaga tccagctctt caccttctgc
    15961 agtcggctga attttgctca gcagtgcgcg gtattgatcc agatcgctgc tttccagctg
    16021 ctgctgtact ttagcggcga atttttccag acggcgtttg cccagcagtt ctgcgttcgg
    16081 cagttctact tccggaatag tcagcttcat agtacgttca atgttgcgca gcagacgacg
    16141 ctcgcggttc tcaacgaaca gcagcgcgcg gccagcacga cccgcacgac cggtacgacc
    16201 gatacggtga acgtaagact cagaatccat cgggatatcg tagttaacca ccaggctgat
    16261 acgctcaacg tccaggccac gggccgcaac gtcggtcgca atcaggatgt ccagacgacc
    16321 gtctttaagg cgttccagtg tctgctcacg cagcgcctgg ttcatgtcac cgttcagcgc
    16381 ggcgctattg tagccgttac gctcaagagc ttcagccact tccagagtcg cgtttttggt
    16441 acgaacgaag ataatcgccg catcaaaatc ttccgcttcc aggaaacgta ccagtgcttc
    16501 gtttttacgc ataccccaca cagtccagta gctctggctg atgtcaggac gggtagtcac
    16561 gctggactga atacgcactt cctgcggctc tttcataaag cggcgggtaa tgcgacgaat
    16621 cgcttccggc atggttgcag agaacagagc ggtctgatga ccttccggga tctgcgccat
    16681 aatggtttca acgtcttcga tgaagcccat gcgcagcatt tcgtcagctt catccagaac
    16741 cagaccgctc agtttagaga ggtccagagt gccacgtttc aggtggtcca gcagacggcc
    16801 cggagtaccg acaacgatct gcggcccctg acgcagggcg cgtaattgca cgtcataacg
    16861 ctggccgccg tacagggcaa ccacatttac gccgcgcatg tgtttagaga aatccgtcat
    16921 tgcttcagca acctgtaccg ccagttcgcg ggtcggtgcc agcaccagaa tctgtggtgc
    16981 tttcagctca ggatcaagat tctgcagcag aggtaaagag aatgctgcag tttttccgct
    17041 ccccgtctgg gccataccca gaacgtcgcg gccattcagc agatgtggaa tacactctgc
    17101 ctgaattgga gatggttttt cgtaacccag atcgttaagg gcttcaagga taggagcctt
    17161 caggcccaga tctgcaaaag tggtttcgaa ttcagccatg tagtacgtgt gcctcaaaat
    17221 taatggcggc cagtctacat aactcatcat gaaattgatc agcaattttc attgaaaagt
    17281 gtgaaccggc tcaaagtagg tgtattaacg aacaacaacg ccctcacccg ttaaggtgat
    17341 ggcaatcaaa aaagattacg ggctgatgtg tacgtcagct attgctggtc cgattctgcc
    17401 aggtcatctt ggtcctggcc caggagcgat aattccaaca atgcgtatcg gtgctcaaca
    17461 aagttatgaa cgttgttggc aaccgccagt ttgaacagtg ccgtggcgct gtccaaatcc
    17521 cccagactta ggtagtactt acctaaatag aagttggttt cactgagatg ctcagcgagc
    17581 gaggtgttat ccgttgcgtc cgccttgagc ctttccatta acgtttgttc gctaatgttg
    17641 cccaggtaga actcgacaat gttccatccc cactgttcct tatccgattt ttcgaagtgc
    17701 tgtttcaaca cttctttagc ctgcttctca tcgagcttct gctcggcgag ataaagccac
    17761 agactacgga aaggatcatt gggatcgtct tgataaaacg ccagcagatc atcttgcgct
    17821 aacttgtcac gaccgccgta atataatgcg atcccgcgat tcaagtgcgc gtagttgtaa
    17881 gttggatcaa gctcaagtac agaatcaaac gcttcatagg cagcatcaaa attgcctgcc
    17941 tgcgttaaat atatgcctaa gtaattgaat acttcaggca tatccggtcg gattgccagc
    18001 gcttgcgaaa aatcgttacg cgctaatgcc ctcagaccga gactatcata caacactccg
    18061 cgctcatata aaagctgtgc gcgttcgtca tcggttaaag cccgactggc aaggatttgt
    18121 tccatacgtg ccagaatcac ttcctgctgt aaagtcggtt gcaatggtac cgcgaggact
    18181 tcacttttac gccaggaagt attactgcat cctgcaagcg taagtgctgt cgcaacgaaa
    18241 caccagcgca aaaaaggctt catttcccac tcccgaagac cacggttgaa tgaacgtcct
    18301 gttcccggtt gctaacaagg cgtcctgccc ggttaaaagc cccccgccgc agcggagggc
    18361 aaatggcaac cttactcgcc ctgttcagca gccggagctt ccggtgctgc agcaggttga
    18421 gactgctcag tcgcttcttt aatgctcaga cggatacggc cctggcgatc aacttccaga
    18481 actttcaccg gtacttcctg acccatctgc aggtaatcgg tcactttctc aacgcgtttg
    18541 tcagcgattt gagagatgtg taccagacct tctttaccgc cgccgatggc aacaaatgcg
    18601 ccaaagtcaa cgatacgggt cactttacca gtgtagacgc agcccacttc gatttctgca
    18661 gtgatctctt cgatacgacg aatagcatgt ttcgctttct cgccgtcggt cgctgcaatc
    18721 ttcacagtac cgtcatcttc gatttcgatg gtggtgccag tttcttcggt cagggcacgg
    18781 ataacagagc cgcctttacc gataacgtct ttgatcttgt ccgggttgat cttgatggta
    18841 tggatacgcg gtgcgaactc agagatatcg ccacgcggcg cgttgatcgc ctgttccatt
    18901 acgcccagga tatgcagacg cgcaccttta gcctggttca gcgcaacctg catgatctct
    18961 ttggtgatac cttcaatttt gatatccatc tgcaatgcag agataccctc gcgggaaccc
    19021 gcaactttga agtccatatc gcccaggtga tcttcgtcgc ccaaaatgtc agacagtaca
    19081 acgtagttgt cgccttcttt caccagaccc attgcgatac ccgcaacggc agctttgatc
    19141 ggcacacctg cgtccatcag cgccagagac gcgccgcaca cggaagccat agaagaggaa
    19201 ccgttggatt cagtgatttc agacacaaca cgtacggtgt acgggaattt gtccatatcc
    19261 ggcatgactg ccagcacgcc gcgcttcgcc agacgaccgt gaccaatttc acgacgcttc
    19321 ggagaaccga ccatgccggt ttcgcctacg gagtacggag ggaagttgta gtggaacagg
    19381 aaggtatcgg tacgttcgcc catcagttca tcaagaacct gcgcgtcacg agcagtaccc
    19441 agcgttgcgg taaccagcgc ctgcgtttca ccacgggtga acagcgcaga accgtgagta
    19501 cgcggcagca cgccagtacg cacatccaga ccacggatca tatctttttc acgaccgtcg
    19561 atacgcggtt cgcctgccag tacgcggcta cgaacaacgt ttttctcgat cgcgtgcaga
    19621 atttcaccca gctcgttttc gtccagggtt tcgtcttcag caagcagcgt cgcgatggtt
    19681 tcagatttga tgacatcaac ctgcgcataa cgctcttgtt tgtcggtgat gcggtaagca
    19741 tcgctcaggc gagcttcagc cagtgcagca acgcgcgcgt tcagcgcttc gtttaccggc
    19801 tccggctgcc agtcccaacg tggtttaccg gcttctttca ccagttcatt gatgttctga
    19861 ataacaacct gctgttgttc atgaccgaac actactgcgc ccagcatctg gtcttcgctc
    19921 agcagttcag cttcagattc aaccatcagt acagctgctt cagtaccggc aacaaccaga
    19981 tccagtttgc tctctttcag ctcgtcctga gtcgggttca gtacgtactg gtcattgatg
    20041 taacctacgc gagcagcacc aatcggacca ttgaacggaa taccagacag agacagtgct
    20101 gcggaagcac caatcatcgc gacgatatcc gggttaactt gcgggttaac agaaaccacg
    20161 gtggcgataa cctgaacttc gttgacgaag ccttccggga acagcgggcg aatcgggcgg
    20221 tcaatcagac gcgcgatcag ggtttcgcct tcgcttgggc ggccttcacg acggaagaag
    20281 ctacccggga tacgaccagc agcgtaggta cgctcctgat agttaacggt cagtgggaag
    20341 aagtcctgac ctggtttggc ttttttctgg ccaacaacgg taacaaatac cgcggtgtca
    20401 tccatgctaa ccataacagc ggcagtagcc tgacgagcca tcatgccggt ttccagagtc
    20461 acggtgtgtt ggccgtactg gaatttacga acgatcggat taagcaatgt aatatccttt
    20521 ctctttctta gacagtacct tacggcactg gtgttaatac ccgatcttct gcgcatcctc
    20581 gcgactaatg acaaccctaa cccagctcta tgtgggtaaa gcctctcatt agccgcgcga
    20641 acctctgcaa cggaagatca ttcatagcaa caatacatta gtttccagtg aattgctgcc
    20701 gtcagcttga aaaaaggggc cactcaggcc cccttttctg aaactcgcaa gaattagcga
    20761 cgcagaccca ggcgctcgat gagctgggtg taacgtgcta cgtctttacg tttcaggtag
    20821 tcgagcagtt tacgacgctg agaaaccatg cgcagcagac cacgacggct gtggtgatct
    20881 tttttgtgct ctgcaaagtg gccctgcagg tggttgatct gtgcagtcag cagtgctacc
    20941 tgaacttcag tagaaccggt gtcgtttgcg tcacgaccaa actcagaaac gattttagct
    21001 gttgcttcag tacttagaga cattttaaaa ctccaaagta tatagaatga aaggacgccg
    21061 atctctaatt cagcgatccc agtgtacgtt acgcaaagtg ttaaacaatt tacgcgacgt
    21121 taagcggcag tattctactc gtagcgacct gttatcgcaa gacggttaac attacgccgg
    21181 gtattcaacc accaggcgac gaggcgcaac gcggccttca tcgtcaattt cgcccatacc
    21241 gataaatttg ccgttctcac cttctgtaac gcgaaccagt ccttccagcg gcgcaccgga
    21301 tgtacgaact gggttaccat ttttgaagta aacagaagac gttaacggaa gattcaccac
    21361 cgggtagtcc gaagctggac tgtccattgg catcagtaat ggatcaagta actccgcagc
    21421 cggaatatcc tgctgttcgg cttgttcgac cagttcacac agatgttcca gggtcaccat
    21481 ccgttcaacc ggatatttac ttaccgccag acgacgcagg taaataacat gcgcgccaca
    21541 accgagtttt tcacccaggt catcaatgat ggtgcggata taagtgcctt ttgagcagtg
    21601 aatttccagc tccagctcat tgccttcgtg gcgaataaac agcaattcat aaacggtaat
    21661 cgggcgcgct tcacgcggta cttcaatgcc ctgacacgca tattcgtaca gttttttacc
    21721 ctgatatttc agtgctgaat acatcgaagg gatctgttcg atatcgccac ggaaagtatc
    21781 cagcgccgcc gccagttgct ctgcgctaaa ggttaccgga cgttcttcaa cgatttgtcc
    21841 gtcggcatca gaagtatcgg tacgctgtcc aagacgcgca atgacccgat agcgtttgtc
    21901 ggagtccagc agatactggg aaaacttcgt cgcttccccg aggcaaattg gcaacatgcc
    21961 agttgccagc gggtccagcg caccggtatg cccggcacgg ttggcgttat aaatccgttt
    22021 cactttttgc agcgcatcat tgctggacat accctgcggt ttatccagca acaaaacgcc
    22081 gttaatgtcg cgaccgcgac gacgaggacg actcattagt cctccttgct gtcgtccggg
    22141 ttaacacgac gttcttcgtc atgtttgacc acgctggtca ccaggtttga catgcgcatc
    22201 ccttcaacca gagagttgtc gtagaagaag gtcagttccg gcacgatacg caggcgcatc
    22261 gctttcccca gcaggctgcg gatgaaacca gaagcttctt gcaacgcttt gatgcccgct
    22321 ttaaccgcgt cttcatcttt gtcgttgagg aacgtcacat atactttggc atacgccagg
    22381 tcgcgagaca tttcgacacc ggaaacggtg gtcatcatgc ccaggcgagg atctttaatt
    22441 tcacgctgca ggatgagagc gatctctttt tgcatttcct gcgctacgcg ctgcgggcga
    22501 ccaaattctt tcgccataat aaattctcct gacaaaaaag gggctgttag ccccttttta
    22561 aaattaattt caggtggaag ggctgttcac gttggcctga taagacgcgc cagcgtcaca
    22621 tcaggcaatc catgccggat gcggcgtaaa cgccttatcc cgcatggaac cctaaaaacc
    22681 ttaagcgatg gtacgttgga tctcgatgat ttcgaatact tcgatcacat cgccagtgcg
    22741 gacgtcgttg tagttcttaa cgccaatacc acattccata ccgttacgga cttcgttaac
    22801 gtcatctttg aagcggcgca gggactccag ctcgccttcg tagataacca cgttgtcacg
    22861 cagaacgcgg atcgggttgt gacgtttaac cacaccttcg gtaaccatac agcctgcgat
    22921 ggcaccaaat ttcggcgatt tgaacacgtc acgaacttcc gccagaccga taatctgctg
    22981 tttcagttcc ggagacagca taccgctcat cgccgctttc acttcgtcaa tcaggttata
    23041 gatgacggag tagtaacgca gatccaggct ttccgcttca atcactttac gtgcagaggc
    23101 atcagcacgt acgttgaagc caaccaggat ggcgttggac gccgcagcca gggtggcgtc
    23161 ggtttcggtg ataccaccta cgccagaacc gatgatcttc actttaactt cgtcagtaga
    23221 cagtttcagc aaggagtcgg agatcgcttc cacagaaccc tgtacgtctg ccttcaggac
    23281 gatgttcact tcgtgaactt cgccttcggt catgttggcg aacatgttct cgagtttaga
    23341 tttctgctga cgcgccagtt taacttcgcg gaatttaccc tgacgataca gtgcaacttc
    23401 acgcgctttc ttctcgtcac gtacaacggt aacttcatca cccgcagccg gtacgccgga
    23461 caggccgagg atttccaccg gaatggacgg acccgcttcc agcacttcct gacccagttc
    23521 gttacgcatc gcacgaacac gaccgtattc gaagccacac agaacgatat cgcccttgtg
    23581 cagagtacct tcacgtacca gaacggtagc aaccggacca cgacctttat cgaggaagga
    23641 ttcgataacc gcaccgctcg ccataccttt acgtaccgct ttcagctcca gaacttccgc
    23701 ctgcagcagg atagcgtcca gcagttcatc gataccagta cccgctttcg cagacacatg
    23761 tacgaactgg ctttcaccgc cccactcttc cggcaggatg ccgtactggg agagttcgtt
    23821 cttaacgcga tccggatcgg cttctggttt atcgatcttg ttcactgcaa cgaccaccgg
    23881 aacctgcgcc gctttcgcgt gctggattgc ttcgatcgtc tgcggcatta cgccgtcgtc
    23941 ggcagcaaca accaggacta cgatgtccgt tgcctgcgca ccacgagcac gcattgaagt
    24001 aaacgcggcg tgccccgggg tgtccaggaa ggtgatcatg ccgttttcag tttcaacgtg
    24061 gtatgcacca atgtgctggg taatgccgcc cgcttcgcca gaggccactt tcgttgaacg
    24121 aatgtagtcc agcagagagg ttttaccgtg gtcaacgtga cccatgatgg tcacaaccgg
    24181 cgcgcgcggt tcagccgcag cacccgtgtc acggtcgctc attaccgcct cttccagctc
    24241 gttttcacga cgcaggataa ctttatggcc catctcttca gcaaccagct gtgcggtttc
    24301 ctgatcgata acctggttga tggttgccat tgcgcccagt ttcatcatcg ctttgatgac
    24361 ctgagagcct ttaaccgcca tcttgttcgc cagttcgcca acggtgatag tttcgccgat
    24421 cacaacgtca cggttaacgg cctgagcagg cttctggaag ccttgctgca gcgaagaacc
    24481 tttacgtttt ccgcctttac cgccacgtac tgctgcgcgt gcttcttcac gatcagcttt
    24541 tgattcagcg tgtttgttgc ctttcttcgg acgcgctgct ttcgcgttac gaccacggcc
    24601 acggccgcct tcgacttcac gatcgctttc gtcttctgcc tggcgagcat gttgagaagt
    24661 agtgacgtga taatcgctgg aatcttcagt cggttccgcg ttatcagtcc atttgttttc
    24721 ttccgccata cgacgtgctt cttcagcaac gcgacgtgct tcttcttcga gtttacgacg
    24781 cgcttcttct tcagctttac gcttgagctc tgcagcttcc tgctcacggc gggctttttc
    24841 agcctgggcg tttttagtca tatcgtcttg ttgattgctc actttgtctt tttccgcagc
    24901 ttcacgtttc gcttgttcag cagcttcacg cttagcttgt tctgcggcct cacgttcagc
    24961 tttttgttgc gcctcgcgtt tagccgattc ttctgcctca cgacgggctt gctcttccgc
    25021 ttcacgctgc gcttgctctt ccgctgcaag gcgttcagcc tcttgcggat cgcgtttcac
    25081 aaaggtgcgt ttcttgcgga cttcgatttg taccgatttg ctttttccac cggtaccagg
    25141 aatgttaagg gtgctgcgtg ttttacgttg cagcgtcaat ttgtccgggc ctgaattttt
    25201 ctgattcagg tggtcaatca aagtctgttt ctcttgtgca gacacagagt cgtcagcaga
    25261 cttccggata cctgcatcag caaattgctg tactaggcgt tccacggagg tctgtcgctc
    25321 tgcggccagc gttttaatcg ttacatctgt catgctgttc cttcctgcta cagtttatta
    25381 cgcttcgtca ccgaaccagc aaatattacg ggcagccata atcagtgctc cggctttttc
    25441 gtcggtcaac ccttcgatat cagccagatc atcaatgccc tgttcggcga gatcttccag
    25501 cgtacaaacg ccacgggcgg ccagtttgaa tgccaaatca cgatctaccc cttcaaggtt
    25561 cagcagatcg tcagccggtt tgttatcacc gaggctttct tcctgggcct gtgcaatggt
    25621 ggccagtgca tttttagcac gctcgcgcag tgcttcaacg gtcggctcat caaggccttc
    25681 gatttccaac agctctttca tcggcacata ggccagttct tccagcgtcg agaaaccttc
    25741 ttctaccaga acagtcgcga agtcttcgtc gatgtcgaga tatttggtga aggtgtcgat
    25801 cgctgcgtgc gcttccgcct gatgcttagc ctgcaggtcg tcaacggtca ttacgttgag
    25861 ttcccaaccg ctcagctgcg aagccagacg cacgttctga ccgttacggc caatcgcctg
    25921 cgccaggtta ccggcttcaa cggcgatatc catggtgtgt ttatcttcat ccaccacgat
    25981 agaagcaacg tctgccggtg ccattgcgtt aatcacaaac tgcgccgggt tatcatccca
    26041 cagaacgata tcgatacgct cgccgcccag ttcagtagaa accgcctgaa cacgcgcgcc
    26101 acgcatacct acgcaagcac ctaccggatc gatacgttta tcgttggttt tcaccgcgat
    26161 tttcgcacga gaacccgggt cgcgagccgc tgctttaatt tcaatcactt cttcgccgat
    26221 ttccggcact tcaatacgga acagttcgat cagcatttcc ggcttggaac gagtgacgaa
    26281 cagttgcgcg ccacgcgctt ccgggcgaac ggaataaagc acgccacgaa cgcggtcgcc
    26341 agggcggaag ttttcacgcg gcagcatatc ttcgcgcagg atcacggctt cagcgttgtt
    26401 gcccagatcc agagagatgt tgtcgcggtt tacttttttc accacgccgg tgatgatttc
    26461 accttcgtgt tcacggaact gatcaaccac catcgcacgt tcggcttcac gcactttctg
    26521 cacgataacc tgttttgccg tctgggtggt gatacggtca aaggtaacag actcaatctg
    26581 atcttcaacg taatcgccca ggttcaggct ttcatcttca taacgtgcgg cttcaagggt
    26641 gatttccttg gtcggctggg tgacttcatc aacaactaac cagcgacgga aggtgtcaaa
    26701 atcaccgctt ttgcgatcga tctgtacacg gacgtcgatc tcttgctcat attttttctt
    26761 tgttgctgtc gccagcgcgc tttccaatgc ttcgaaaatc ttctcgcgag gtagcgcctt
    26821 ttcattggat acggcttcaa ctacagccaa aatttctttg ttcatcgcgg gcttttcacc
    26881 tcatccagac tattaaaagt ggggaaccag gttcgccttc tggatattac tcagcgcgaa
    26941 cacttcatct ttaccttcga cggtaactgt gatcatttca ccgtctaccg ctttgataac
    27001 gccctgccat ttacgacggt tttgtaccgc catacggaga accagagtca cctcttctcc
    27061 gacaaaacgg gcgtagtgtt cagccgtgaa cagtgggcga tcgagacccg gtgaggagac
    27121 ttccaggtta taagcaacgg tgatgggatc ttcaacatcc agcacagcac ttacctggtg
    27181 gctcacatca gcacaatcat caacattgat gccatcttca ctatcaatat agatgcgcag
    27241 tgtggatgtg cgaccgcgaa taaattcgat gcctaccagt tcaaaaccca gggcctcaac
    27301 tggcgcagta atcatctctg ttaatttttg ctctaatgtg gacaagccca cccccaagac
    27361 ataaaaaaag ggcctaaagc ccagttattc tgtagtcaga taacaaaaaa ccccgataaa
    27421 tcggggcttt atataactga accctataac cgcaactgcg gtctggagca ctttccagaa
    27481 ggattttttc aaatcccact acgaaggccg aagtcttcac agtatatttg aaaaaggact
    27541 ctaagggaaa gtggttgcgg gggccggatt tgaaccgacg accttcgggt tatgagcccg
    27601 acgagctacc aggctgctcc accccgcgcc tgaaacgtgg caaattctac tcgttttggg
    27661 taaaaaatgc aaatactgct gggatttggt gtaccgagac gggacgtaaa atctgcaggc
    27721 attatagtga tccatgccac attttgtcaa cgtttattgc taatcacgtg aatgaatatc
    27781 cagttcactt tcatttgttg aatacttttg ccttcccctg ctctccctta agcgcattat
    27841 tttacaaaaa acacactaaa ctcttcctgt ctccgataaa agatgattaa atgaaaactc
    27901 atttattttg cataaaaatt cattgagagc ggaaatccag gctcatcatc agttaattaa
    27961 gcagggtgtt attttatgac gacgattctc aagcatctcc cggtaggtca acgtattggt
    28021 atcgcttttt ccggcggtct ggacaccagt gccgcactgc tgtggatgcg acaaaaggga
    28081 gcggttcctt atgcatatac tgcaaacctg ggccagccag acgaagagga ttatgatgcg
    28141 atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga ctgccgcaaa
    28201 caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa caccaccggc
    28261 ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggtac catgctggtt
    28321 gctgcgatga aagaagatgg cgtgaatatc tggggtgacg gtagcaccta taaaggaaac
    28381 gatatcgaac gtttctatcg ttatggtctg ctgaccaatg ctgaactgca gatttacaaa
    28441 ccgtggcttg atactgactt tattgatgaa ctgggcggcc gtcatgagat gtctgaattt
    28501 atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcctactc cacagactcc
    28561 aacatgcttg gtgcaacgca tgaagcgaag gatctggaat acctcaactc cagcgtcaaa
    28621 atcgtcaacc cgattatggg cgtgaaattc tgggatgaga gcgtgaagat cccggcagaa
    28681 gaagtcacag tacgctttga gcaaggtcat ccggtggcgc tgaacggtaa aacctttagc
    28741 gacgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg cctgggcatg
    28801 agcgaccaga ttgagaaccg tatcatcgaa gcgaaaagcc gtggtattta cgaagctccg
    28861 gggatggcac tgctgcacat tgcgtatgaa cgtctgttga ccggtattca caacgaagac
    28921 accattgagc agtatcacgc gcatggtcgt cagttgggcc gtctgctgta ccaggggcgt
    28981 tggtttgact cccaggcgct gatgctgcgt gactctctgc aacgctgggt tgccagccag
    29041 atcactggtg aagttaccct ggagctgcgc cgtgggaacg attattcaat cctgaatacc
    29101 gtctcagaga acctgaccta caagccagag cgtctgacga tggaaaaagg cgactcggtg
    29161 ttctcgccag atgatcgtat tggtcaattg accatgcgta atctggatat cactgatacc
    29221 cgagagaaac tttttggtta tgccaaaact ggcctgcttt cctcctctgc cacttcaggc
    29281 gtgccgcagg tggagaatat ggaaaacaaa ggccagtaat tcgacatcaa ccctgccctt
    29341 caggggtggg gttaccttgc ttatcctctg taaactcctt tctgagcatc acataatcga
    29401 aaacatcatg gtttgcatcc acaacccgtg agtctccttt atagtgtgca atcacttcct
    29461 caagcgtttg cggctgttcc ggctttgtta cccccatcca cgcattaata aggtaattat
    29521 tgtaagctgt cgaaaagata ttgttttccg ttgtgcgatc cacgccatcc ccaagaacgg
    29581 ggctatacca gataaacatc gggacatgat atgcctgctg gctagcctcc ctgcctccat
    29641 gaaaatagac gttcttcttc gtagggtcac gttccaggcc gtggtcggca aaatacatga
    29701 ccgaggcgcg gcgatctttt aataattcaa aaacctgacc tagcaaacta tctgtgtaat
    29761 gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg ttgaaacacg gcgctggatt
    29821 gcggataggc gctacaagcc ggttcatggc ttccatttaa atgaagaaca atcagtttct
    29881 tttgctgcgt attttgctgt aatgcttgcg ataaatgcgg caacaacaat tcatcaaatc
    29941 ctctgacata aactgtttcc atggcgcgca tggcgatact ggtaactgct gtaccattct
    30001 gccgaaaagc ggattgcgag cttagccaga aagtctgaaa tcctgcttga ttagccatat
    30061 taataatgtt gtcggggtaa ttatgaatgt catgactcaa aacagaatca gcagttaaag
    30121 aaaggggaac cgacagcgca gtgtaaggtg cgccgcttat tgcttgatta aacagtttga
    30181 tctgttttct ttgtgcttca acttgcggtg tcgtagagcg tgtatatcca tacaaagaca
    30241 tattgtcgac acgtacagac tcccccacaa tcaacacgta ggtatcaata cctgtatccc
    30301 tgactgataa ttgaaaatac ggcaccgtgt ttgcaattga gagtaatctt tgatgctctt
    30361 tcgctgctaa agcaaaatag ttgagattga aaaacggcgt ataggtagca aa
//
LOCUS       DBKEKI010000039        30258 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.039, whole genome shotgun sequence.
ACCESSION   DBKEKI010000039 DBKEKI010000000
VERSION     DBKEKI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.039"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..625
                     /gene="ypdB"
                     /locus_tag="ACUQPX_001720"
     CDS             <1..625
                     /gene="ypdB"
                     /locus_tag="ACUQPX_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HHV9056459.1"
                     /translation="VLKFLQHNRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFIT
                     AWKEHAVEAFELEAFDYILKPYQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTI
                     NLVKDERIIVTPINDIYYAEAHEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHR
                     SFCVNLNKIREIEPWFNNTYILRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            638..1495
                     /locus_tag="ACUQPX_001721"
     CDS             638..1495
                     /locus_tag="ACUQPX_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9056460.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(1498..3993)
                     /gene="ptsP"
                     /locus_tag="ACUQPX_001722"
     CDS             complement(1498..3993)
                     /gene="ptsP"
                     /locus_tag="ACUQPX_001722"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HHV9056461.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQSTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPD
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDLSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(4018..5054)
                     /gene="ypdE"
                     /locus_tag="ACUQPX_001723"
                     /pseudo
     CDS             complement(4018..5054)
                     /gene="ypdE"
                     /locus_tag="ACUQPX_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(5054..6137)
                     /gene="ypdF"
                     /locus_tag="ACUQPX_001724"
                     /pseudo
     CDS             complement(5054..6137)
                     /gene="ypdF"
                     /locus_tag="ACUQPX_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(6152..7399)
                     /locus_tag="ACUQPX_001725"
     CDS             complement(6152..7399)
                     /locus_tag="ACUQPX_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HHV9056462.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(7421..7747)
                     /locus_tag="ACUQPX_001726"
     CDS             complement(7421..7747)
                     /locus_tag="ACUQPX_001726"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HHV9056463.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(7966..8931)
                     /gene="glk"
                     /locus_tag="ACUQPX_001727"
     CDS             complement(7966..8931)
                     /gene="glk"
                     /locus_tag="ACUQPX_001727"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HHV9056464.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            9135..10391
                     /locus_tag="ACUQPX_001728"
     CDS             9135..10391
                     /locus_tag="ACUQPX_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HHV9056465.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            10506..10832
                     /gene="ypeC"
                     /locus_tag="ACUQPX_001729"
     CDS             10506..10832
                     /gene="ypeC"
                     /locus_tag="ACUQPX_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HHV9056466.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(10973..12211)
                     /locus_tag="ACUQPX_001730"
     CDS             complement(10973..12211)
                     /locus_tag="ACUQPX_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HHV9056467.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(12192..12248)
                     /locus_tag="ACUQPX_001731"
     CDS             complement(12192..12248)
                     /locus_tag="ACUQPX_001731"
                     /inference="COORDINATES: protein motif:HMM:NF049535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056468.1"
                     /translation="MKHSKGYVLEAQDDELSR"
     gene            12547..13749
                     /gene="nupC"
                     /locus_tag="ACUQPX_001732"
     CDS             12547..13749
                     /gene="nupC"
                     /locus_tag="ACUQPX_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HHV9056469.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(13799..15988)
                     /locus_tag="ACUQPX_001733"
     CDS             complement(13799..15988)
                     /locus_tag="ACUQPX_001733"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HHV9056470.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGNLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(16196..16271)
                     /locus_tag="ACUQPX_001734"
     tRNA            complement(16196..16271)
                     /locus_tag="ACUQPX_001734"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16236..16238),aa:Ala,seq:ggc)
     gene            complement(16311..16386)
                     /locus_tag="ACUQPX_001735"
     tRNA            complement(16311..16386)
                     /locus_tag="ACUQPX_001735"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16351..16353),aa:Ala,seq:ggc)
     gene            16607..16966
                     /locus_tag="ACUQPX_001736"
     CDS             16607..16966
                     /locus_tag="ACUQPX_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HHV9056471.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            16968..17360
                     /locus_tag="ACUQPX_001737"
     CDS             16968..17360
                     /locus_tag="ACUQPX_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HHV9056472.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(17412..18827)
                     /gene="gltX"
                     /locus_tag="ACUQPX_001738"
     CDS             complement(17412..18827)
                     /gene="gltX"
                     /locus_tag="ACUQPX_001738"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HHV9056473.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            19086..19161
                     /locus_tag="ACUQPX_001739"
     tRNA            19086..19161
                     /locus_tag="ACUQPX_001739"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19119..19121,aa:Val,seq:tac)
     gene            19206..19281
                     /locus_tag="ACUQPX_001740"
     tRNA            19206..19281
                     /locus_tag="ACUQPX_001740"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19239..19241,aa:Val,seq:tac)
     gene            19286..19361
                     /locus_tag="ACUQPX_001741"
     tRNA            19286..19361
                     /locus_tag="ACUQPX_001741"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19319..19321,aa:Lys,seq:ttt)
     gene            19481..19822
                     /locus_tag="ACUQPX_001742"
     CDS             19481..19822
                     /locus_tag="ACUQPX_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HHV9056474.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(19813..20739)
                     /locus_tag="ACUQPX_001743"
     CDS             complement(19813..20739)
                     /locus_tag="ACUQPX_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHV9056475.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRGAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            20829..21827
                     /locus_tag="ACUQPX_001744"
     CDS             20829..21827
                     /locus_tag="ACUQPX_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HHV9056476.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIADGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(21824..22042)
                     /locus_tag="ACUQPX_001745"
     CDS             complement(21824..22042)
                     /locus_tag="ACUQPX_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HHV9056477.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(22044..24059)
                     /gene="ligA"
                     /locus_tag="ACUQPX_001746"
     CDS             complement(22044..24059)
                     /gene="ligA"
                     /locus_tag="ACUQPX_001746"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HHV9056478.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(24130..25116)
                     /gene="zipA"
                     /locus_tag="ACUQPX_001747"
     CDS             complement(24130..25116)
                     /gene="zipA"
                     /locus_tag="ACUQPX_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HHV9056479.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            25346..26107
                     /gene="cysZ"
                     /locus_tag="ACUQPX_001748"
     CDS             25346..26107
                     /gene="cysZ"
                     /locus_tag="ACUQPX_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HHV9056480.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            26292..27263
                     /gene="cysK"
                     /locus_tag="ACUQPX_001749"
     CDS             26292..27263
                     /gene="cysK"
                     /locus_tag="ACUQPX_001749"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HHV9056481.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            27647..27904
                     /gene="ptsH"
                     /locus_tag="ACUQPX_001750"
     CDS             27647..27904
                     /gene="ptsH"
                     /locus_tag="ACUQPX_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HHV9056482.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            27949..29676
                     /gene="ptsI"
                     /locus_tag="ACUQPX_001751"
     CDS             27949..29676
                     /gene="ptsI"
                     /locus_tag="ACUQPX_001751"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HHV9056483.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            29717..30226
                     /gene="crr"
                     /locus_tag="ACUQPX_001752"
     CDS             29717..30226
                     /gene="crr"
                     /locus_tag="ACUQPX_001752"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HHV9056484.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         7433 a         8016 c         7510 g         7299 t
ORIGIN      
        1 cgtgttgaag tttttgcagc ataaccgcgt cgacgccatt tttctggata tcaatattcc
       61 gtcgctggat ggcgtgttgc tggcgcaaaa catcagccag ttcgcccata aaccgtttat
      121 tgtgttcatc accgcgtgga aagaacatgc ggtagaagcg tttgaactgg aggcgtttga
      181 ctacattctc aaaccgtatc aggagtcacg tattaccggg atgctgcaaa aactggaagc
      241 ggcctggcaa caacagcaga ccagcagtac gcctgccgcg acggtaacgc gtgagaatga
      301 caccattaat ctggtgaaag atgagcgaat aatcgtcacg ccaattaacg atatctatta
      361 cgccgaagcg cacgagaaaa tgacctttgt ctatacgcgg cgtgaatcct acgtaatgcc
      421 gatgaacatt accgaatttt gcagcaaact gccgccgtcg cattttttcc gctgccatcg
      481 ctcattttgt gtcaatctga acaaaatacg cgaaatcgaa ccgtggttta ataacaccta
      541 cattctgcga ctgaaagatc tggattttga agtgccggtc agccgcagca aagtgaaaga
      601 atttcgccag ttaatgcatc tttaatcggg aactttcatg aaagcaccag gcttgcccgc
      661 cgatcaacaa tttttcgccg atctgttcag cggcctggtg cttaacccgc aactactggg
      721 gcgcgtctgg tttgccagcc agcctgcctc gttaccggtg ggcagtttat gtattgattt
      781 tccccgtctg gatatcgtgc tgcgcggcga atacggcaac ctgctggaag caaagcagca
      841 gcgaatggtg gaaggagaaa tgctgtttat tccggcacgc gcggctaatt taccgatcaa
      901 caacaaaccg gtgatgctgt taagcctggt gttcgcaccg acctggcttg ggttatcgtt
      961 ttacgatagc cgcaccacgt cgttgttgca tcctgctcgc cagatccagc ttcccagcct
     1021 gcaacgcggt gaaggtgaag cgatgcttac cgccctcacc catcttagcc gttcgccgct
     1081 ggagcagaat atcattcagc cactggtgtt aagtttgctg catctttgcc gcaacgtggt
     1141 gaatatgccg ccgggtaatt cgcaaccgcg cggcgatttt ctctatcaca gcatttgtaa
     1201 ctgggttcag gataattatg cccagccgct cacccgcgag agcgtggcgc agttttttaa
     1261 tatcacgccc aatcatctgt caaaactgtt tgctcagcat ggaacgatgc gttttatcga
     1321 gtatgtgcgt tgggtgcgaa tggcgaaggc gaggatgatt ttgcagaaat atcatctgtc
     1381 aattcatgaa gtggcacagc gttgcggttt tccggatagc gactattttt gtcgcgtttt
     1441 ccggcgtcag tttggtctga cgccgggaga gtacagcgcc cgttttcagg gctaacgtca
     1501 gaaggttaat tctgtttcca gcagcgtcag gatactttgt gcatcttgcg cggcaaacaa
     1561 cgactggcgg aagtttttat tcaccagttt acgcgccagc tgcgagaaga ctttcacatg
     1621 attcatccct tcgttagcac ccagcgtcag catgatcacc agttcgactt cgcccatttc
     1681 tgactgccag tcaaccggtt tcgccagccg ggcaatgctg atactggaat gacggatcca
     1741 ctgagatttg gtgtgcggga tcgctacgcc aaaaccaacg ccggtggtaa caatctcttc
     1801 ccgctgccag acatcttctt ccagctcaaa cggatgttca gtgcgcccgt taacgccgag
     1861 gttgccgcac aggaactgga tcgcctgctc tttattgcta agatcctgat caacaaagat
     1921 attctccagc gccagcagtg ggcgaacgtc ttcttccggc gtaaaggcgg tgagtaacgc
     1981 ttcaatttcc tgcgcactgc ggcattcaca tgcctgacgc gccagttccc gacacgcctc
     2041 gctatccagt tgacgaagct ggcttttcac cgccggaata cgcgggctac tcatactcag
     2101 ctcatccagg cccagcccaa gcagtagcgg cagataacgg ctttcaccgc ccagttcacc
     2161 gcaaatgcct acccatttgc cccgctgatg cgcagtggta actatttgct gcaacatgcg
     2221 caggaacgat ggcgtaatcg ggttatatag cggcgatacg cgcgggttat tacgatcgac
     2281 cgcatacaga tactgggtca tatcgttgga gccgatactg aagaaatcga cctcatcgca
     2341 gaagtggtcg atgatgtagc acaccgacgg cacttccacc atgatcccaa gcgtaatcgt
     2401 ctctgcatga cgcaggccat cgcgcttaag ctcaacgatc gctttttgga tctcgccttt
     2461 cacccataag atctgatcga ggctgtgaac catcgggatc atcaactggg cgttgccgaa
     2521 actggcggcg cgcaaaatgg cccgcagttg agtgcggaac aggccagcaa attccgggta
     2581 aatacgtacc gcgcgatagc cgaggaacgg gttctcttcc tggggaatat tcagataagg
     2641 aatgctttta tcgccgccga tgtccatcgt gcggaagata atcggcttgt cgcccgccgc
     2701 cagtagcacc tgctggtagg cttcaaattg ctcctgctcg tccggcgcgc tgtcgcgatc
     2761 catatagagc atttcggtac ggaacagccc gacaccttcc gcgccgttgg caaacgcgtc
     2821 tggcgcttcc agagcggtgc cgatattcgc cgcaatatca atacgcttgt tatcacggga
     2881 ataggccagc tgcgcggcag cctgcgcctg ttgtttttgg cgtttatccg ccagcgtctg
     2941 cgcgacctga taataaccgc tcaccgcgtc attcgggtta atcgccagca cgccgcactg
     3001 ggcgtcaagc actgcaggtt gcccggcata acgggcaatc gcatccagcg gcaagccact
     3061 cagtaccggg atcgccgagg cacgggccag aatcagtgta tgcgaggtgc gcccggtttt
     3121 ctccagaatc atacccgcaa gatttttcaa atcaaggctc aaaaactggc ttggggttaa
     3181 atcttcagcc accagaatgg tcggtttttc cagcaccaga ttgttgcgcg gcttcagttc
     3241 cggccaggtg atatgcagca actgttcgct gatatcgcga atgtcgctga cccgctcgcg
     3301 cagataatcg ctggcagaag cagatagttt ggcgcaaacc tgctccatat tgctgatgat
     3361 cgccgccccc agtccctgat gctgttctgt catcaggcga cggatattgc ctgcaaattc
     3421 atcatcctga atcagcgaca aatgagcgct gaggatagtt ttgctttcgc cgtcacgctc
     3481 acgcaattgc tggttcagtt gctcggcaag cgttgccagg ctgtgctcca gccgggtgga
     3541 atcttgcgca ctggcgggga ttgcccgata actgtcgagg ctgtcgcttt gtaacagggt
     3601 cagcgtaccc acgccgacgc cgcttgccag cacattgccg tacagtaaat ccgggttaag
     3661 gcggcttaat gaacgcggca gcggatgcgc cgtcagttct gcctgcgtcg actgaacgct
     3721 gtcgctgtcg ataaagcgca ccaggatgta ctcttccagc acgcgccgcg cctgctcttc
     3781 atcgctgccg ctaatgttca ggctgcaact gtcattaaat aaggtgccag ttccaatcaa
     3841 cgccagcgag cttttagcat cggcctttgc gttctggcga tggttgataa aggtgatttc
     3901 gctttgccac tggctgcact gttctttaag ttcccacgcc ggacgagcat gtagaccgtt
     3961 aggcaaagga cagagaaatt gaatcgttaa cataaccgct ccgggttaat caggagatca
     4021 tctgaaatcc gtcagttgaa caaccgtctc acgcgtaaga cgttgaataa gggcagataa
     4081 aagttgctcc atctgcaaaa tgtcgcggca atcggcaatc gatgccgcgc aatgtccgtg
     4141 gcgggtggca ggccccatta ccactgtggg tacgccagta ccggttaagt gcaccgctcc
     4201 accgtccgtg ccgccgttac tgaacatatc cgcctgcaac ggaacgccaa tttctgccgc
     4261 cacggtttca atccacgcgg taagttttgg cggcgcaatc agcgatttat cgcttaacac
     4321 cagcatcggc ccgttaccaa tctgacgatg gttagccgcg ccataatcaa agtttttcgc
     4381 ccagcaggcg gtatcaagca caatggcgac gtccggcgac accgcgcggg tagcagtttg
     4441 cccgccgcgt aatcccacct cttcgctgga acttgccacc agccacactt ccgcaggcag
     4501 ttcggcgtcg tgcagctcac gtagtaacgt caccagcaga tagcaaccga ggcgatcatc
     4561 aaaggcttgc ccatcacccg ctggtgaggg aggacctgaa aagtggtatc gaaggtgacg
     4621 cgatcgcctg gacgaattcc cgcctgcatc acttcgtcat aggtgcgcgc gccaatgtcc
     4681 acgcgcatgg cgctaacgtc attcccctgc cgttcgccgt caagcaggcc tggaattttg
     4741 cgctcttcac gggtggtgat gcgcaccggc tgcagctggc gggcagccat gcgcacgttg
     4801 ccaaccggca gcacgtcaat agcgccttcg cgggagatgc tgcgcaccat aaatcccact
     4861 tcgtccatat gggcgcagat catcaccttc ggacctgtcg attcattgag gcggatcagc
     4921 accgatccca gaccatcaaa tcgcacttct ttttgcaggc gagccgcttc ttccagcagg
     4981 atctgccgca cttcctgttc cgaggaggcg atcgcatctg cctcgctcaa cgcttttaat
     5041 agcgataaat ccattatgcc tctcccgtga gcaacactgt tttcggcatg gcgtagagca
     5101 cttctgcgcc ttgcggggta accagcacaa catcttcgat gcgcacgccc ccttgcccta
     5161 gcaaataaat ccccggctcc acggtcagca acatgcctgg ctgtagcgtc gtggtgtccc
     5221 gcggtgaaaa acgcggatct tcatggactt caatgccgat agcgtgacca gtgttatgac
     5281 cgaaatagtc gccaaatcca gcctcggtaa tgacccggcg cgcggcgtcg tcaatctgct
     5341 ggcagcgcac gccggggcga attgcagaga ttgccgcgag ctgtgcctgc aggacaatct
     5401 gatagacgtc aaacagcggg tgagattcgg cgctcacccc ttcgccattc accagcaagg
     5461 tgcgcgtcat atcagcagta gccctgatac agcgcaccaa aatcgagagt gacaaactcg
     5521 cccgctgcaa caatcttgtc gctggctttg ccgtgcggca gcgccccgcg ccagccgctg
     5581 gcgacaatgg tgtcaaaaga ggctttttct gcgccctgct ggcgcataaa ccactccagt
     5641 tcagcggcta tctcgcgctc gctcatcccc gcctgaataa agcggcgaat atgctccgct
     5701 ccgcgatcgg caatcccaca ggcgaggcgg attatctcca cctcctctgg cgttttgatt
     5761 tgccgcagca catccggcgt ggcgctaacc agtttcgcat tgagttcaga cttccagcgg
     5821 tgcgcggttt cccaactcac ctgctggcct tcaaaaccga gcgtttgcaa ctgctcatcg
     5881 gcaatgattt gattgacgat agtggtaagc gtgttcgtcg cgtcaagcaa atgcagctgg
     5941 tagccttgcg cacgggcttc cacctcaacg aaatagcgcg aatctaccag aatgtgcgca
     6001 ctttcacggc taataaccac atagcctgat ccagtggaga tccccagatg cggctgtttg
     6061 ttctgccgtg aggagagaag caccgcatcc agttgttgca ccttaagcca gtcgcgcagc
     6121 gaagcgagta atgtcatctg gattttcctt cttacaggct atcgattaac aatttgcctt
     6181 tgcggaacat catcagacgc aggaacacca ccatcagcgc agtaatgatt gcccccagcg
     6241 cgatccccgc catataaacg cccaggttag tgaccagcgg ccatgcccag atagcggatt
     6301 ccgggaacca ttgcactgcg cccagccaga cagctgcggt tgagccgaca atcgcgccga
     6361 ccatatatga cggaatggcg gtgatggggc tttccagcgc aaacggaatc gcaccctcac
     6421 taatccccat aaaggccagg aacatcgcgg tcttaccctg cggataaagt tgagcgttga
     6481 acaggcgttt gccggttaaa cgacggtcaa taatggtcgc cagacccaga ccaatcggcg
     6541 gaataacgat agcgatagaa cgagcggtga ccggtaatac gtggtcagtg gtaaagctga
     6601 aggcaacgaa acctgcggct ttgttgatcg ggccaccaag gtcgattgct gtcgccgcgg
     6661 cgatccccat tgcgtacatc agcgcgccct tctcacctgc ggcagtcagc acagtacgga
     6721 taccgccgtt gatccagcca ccaaaggggg tgatgacgta gtacatcgcc agcatgacaa
     6781 aaatggcgga aagaatcggt agcaaaaatg tggttttgaa cgctaacagg aaatccggta
     6841 actggatttt ctggttcatc catttcacaa ggtaaccggc aacgatagaa ataatcagcg
     6901 caccaatgaa ggtggacggc accggcgaag aggtcgccca ttgcatagtg ctgggatcga
     6961 agttcagcag ttgcgtcggc tgggtagaca tcaacccgcc gataaaacca gccgggaacg
     7021 ccagtttgcc accgatagag ttcgccacaa atgcagcaaa catcggaatg gcaaaaccaa
     7081 acaacacgcc gccgaaggac tgcgacagcc aggcaaattt aagtaaggag aggtcgaaac
     7141 cggagaattt cccgctatta agagcatcca tgatgccgat ctcagcagga attttaagcc
     7201 agctataagc aatcagctgg ctgaaagcga ggatcacacc gcccataatt aaggtcggca
     7261 ccatgcgtga aatccctgac atcacatgct gcgggagttc accccagaag ctgcttttag
     7321 aggcctgcgg attcttaact gccgctgccg caccggatgc gccaggcaca acggttgcac
     7381 tgcgtttttt aatggccata acaattatcc ctgtcgaata ttactgttgt tcagaggcaa
     7441 tcatctcttc gatttctttg atgatgcccg cagcgttttt aattgcgtcc tgcaaagtga
     7501 tttcataaac gtcgcgtgat tcgaaacgtt cgttatcttc cggggtaact gccacggaat
     7561 ggatgatgat ggtcgcttcg gcgatatcct gcgccgtcag gcgattctgg atgccgtccg
     7621 cgccctgggt ttcaattttc acttcataac cggcttctac cgccgcttct tccagcgcct
     7681 gagcggccat aaaagtgtga gccaggccca ttgggcaggc acataaggca atcagtttct
     7741 tactcatcgt taattcctcg ttcagtgata gttgacgagt gaaattatga acgtgcaaaa
     7801 caaatcgccg ttaccagaca aataatgcat ttactggaga attaatcccg ctgtacggaa
     7861 gtgtgacgca ggtcggcctt gtgaggggac acaaagaaaa attgaattaa ctgtatgatt
     7921 taaaagatta tcgggagagt tacctcccga tataacagga aggatttaca gaatgtgacc
     7981 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggatagtcat ggacgatgag
     8041 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg
     8101 gaaaccggag gctttgaaga actcaaggaa gcgcggcacg ataccgcccg caataaacac
     8161 gccgccaaat gtcccgagat tgagcgccag attgccgcca aaacggccca taatgacgca
     8221 aaacagcgac aatgcgcggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
     8281 atcttttggc ttgagatttt ctggcaggcg gttgtcagct ttcacaattg cgcgatacaa
     8341 attcaccagc ccagggccag aaagcacgcg ctccgccgaa acatgaccaa tttccgcacg
     8401 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
     8461 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
     8521 cgttccggca ccgtaaaccg caataggctt accttcgacc ggttctgcgc caccaaactg
     8581 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
     8641 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
     8701 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
     8761 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
     8821 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
     8881 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
     8941 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
     9001 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacaatta ctttatcgtt
     9061 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
     9121 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
     9181 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
     9241 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caggattcac
     9301 ccctctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
     9361 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccggttc
     9421 cgccctctgc gctacctgga cttatcgtag cattaattct cggtcttgct ggcggcgtca
     9481 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
     9541 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
     9601 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
     9661 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
     9721 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
     9781 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
     9841 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa
     9901 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
     9961 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    10021 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    10081 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    10141 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    10201 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    10261 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    10321 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    10381 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    10441 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    10501 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    10561 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    10621 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    10681 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    10741 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    10801 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    10861 gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc tctttaacgc gtagggcgga
    10921 taaggcgttt acgccgcatc cgccaatagt gccagatgcg acgctcattc aactacaatc
    10981 ccagcgccgt ccccaccaac aaccagatat tcagcgccac gaccagcact acaatcaccc
    11041 agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc catcaacttg ctgtcactgg
    11101 tgaaaatcag cagaggaacc agcgccaggg cgataccaaa acttaacagc acctgactca
    11161 taaccagaat ccgtgtcgga tctaatccca tcagaatgac aataaatgac ggcaacatgg
    11221 tgactgtacg acgcacccac agcgggatat ggaagcgaat gaatccctgc atcaccacct
    11281 gccccgccag tgtccccacc accgttgagg acagtccggc agcaaccaga cttaacccaa
    11341 agaccgttgc cgcagcatgg cttaacagcg gttgcagcgt cagataagcc tcatcaagat
    11401 cggcaacacc agtatgacca gaaaagtgga acgccgccgc agctgtagcc atcatcgcca
    11461 gattgacaaa accggcaatc gtcatggcga tagccacatc ccatttggtg gcggaataac
    11521 gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa taaatcacat
    11581 gcggcataat cgtcgcccct aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta
    11641 aactcgggat caccattcct ttacccagct gcgccaggtt aggctgggag aaaatcaact
    11701 cgacaatgta agccgcggca acaaacaaca gtaacccgcc aatcactttc tccagcggtt
    11761 tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc
    11821 cctgcaacaa cgaaacacca agaatgagtt taaaaccgat cgccgcacca ataaattccg
    11881 ccaggtcggt tgccatcgca ataatttctg cctgaaccca atagaaccac actacgggac
    11941 gcggatagtg atcacgaatc tgctccgcca gatttttacc ggtggcaatc cctagtttgg
    12001 cagagaggat ctgaatcagc atcgccatca ggttggccca aacgacaacc cacagtagct
    12061 gatagccgaa gctagcaccc gcctgaatat tggtcgcaaa gttaccggga tcgatataac
    12121 caatcgccgc aatgaacgca ggtcccatta atgcgagcct catcttgcgc gccgcccgtc
    12181 cgctgctact ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata gcctttgcta
    12241 tgtttcatgc tatgctaaac gagaatgatt atcaaattca tttaaatgga ttgtggtgat
    12301 ttctctgata gaccaggatt atgattacga aaagattgat gacttgaatg ttatggttat
    12361 gtttaatgtt agcacattta cataactttc agcttccata cacaacatag cagaaatgta
    12421 tgacagatca ctatttttga agcctgtcac aggacgtcat tatagtgtgt gtcagatctc
    12481 gttttcctta accatgttac atagaatgtg cacggaaatt taacctgcct catatttgga
    12541 gcaaatatgg accgtgtcct tcattttgta ctggcacttg ccgttgttgc tattctcgca
    12601 ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt atgttattca actgcttgtt
    12661 atcgaagtgt tactggcgtg gttcttcctg aactccgacg ttggtttagg cttcgtgaaa
    12721 ggcttctccg aaatgttcga aaaactgctc ggatttgcca acgaagggac taacttcgtc
    12781 tttggtagca tgaatgatca aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc
    12841 gtctttatct ctgcactgat cggtattctc cagcacattc gcgtgttgcc ggtgattatc
    12901 cgcgcaattg gtttcctgct ctccaaagtc aacggtatgg gcaaactgga atcctttaac
    12961 gccgtcagct ccctgattct gggtcagtct gaaaacttta ttgcctataa agatatcctc
    13021 ggcaaaatct cccgtaatcg tatgtacacc atggctgcca cggcaatgtc caccgtgtcg
    13081 atgtccatcg ttggtgcata catgaccatg ctggaaccga aatacgtcgt tgctgcgctg
    13141 gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga tcaatcctta ccgtgttgat
    13201 gccagtgaag aaaacatcca gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg
    13261 ctgggtgaat acattctggc aggtttcaaa gttgccatta tcgttgccgc gatgctgatc
    13321 ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg ccaccgttac tggctggttt
    13381 ggctacagca tctccttcca gggcatcttg ggctacatct tctatccgat tgcatgggtg
    13441 atgggtgttc cttccagtga agcactgcaa gtgggcagta tcatggcgac caaactggtt
    13501 tccaacgagt tcgttgcgat gatggatctg cagaaaattg cttccacgct ctctccgcgt
    13561 gctgaaggca tcatctctgt gttcctggtt tccttcgcta acttctcttc aatcgggatt
    13621 atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt
    13681 ctgaaactgg tttacggctc taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg
    13741 gtgctgtaaa accatacata aaaaagccgg ggatcattcc ccggcttttt tacgcctgtt
    13801 aatcagctaa tggctgcggg cgaccaatca aatacccttg cagatattgc accccgagct
    13861 tatgcaatag cgcctgctgc tgttgcgtct cgacaaactc cgcgaccaca ctcaatgact
    13921 tcgctttcgc cagatcggta attgatctca caatcatcgc atccagcgta ttcgtgacaa
    13981 tatctttcac aaagacgcca tcaattttga tgatatcagc ctgcaaacgc tttaaccgtt
    14041 cgtagttggc atatccggtg ccaaagtcat caatcgcaat ccggaaacca aacttatgca
    14101 gctgctcgat gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc tcggtgatct
    14161 caagaatgac cgcctgcggg gagatgtgat aacgcttaaa cagacgaata atccgcccgg
    14221 caatattctt ttgcagcagc gtgagcggca ttaaattgac tgaaaagcgc ggtccttttt
    14281 tgtcgcaagg gtgtgttgcc agccacttca acaaggattc cagcacttgc aaatcaaaac
    14341 gcgcgctaag gttaaactga gcaataaggg gcagaaactt atccggggtc ataatgccgc
    14401 cgtcatattt cagtcgcgcg aggatctcat cataaccttc accctctttg ttgcgaattg
    14461 gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta ttcagcaata
    14521 gcacctgttt agtggtctgc ccggaaacca tctcctctct gctatccagc gccagcacat
    14581 gatgatgtgc gcaggattgc tccgccagcc agcttaactg ccccaacaag ggttgcaggg
    14641 tttcctgatt tccatcaaaa cgcccccagg cagcaccata gcccatatcc agcccggtat
    14701 tgttccagtg aatttgccga ctattcagga tgttaaccat atgctggagt cgcccttccg
    14761 tttccggccc gctcagcact aacagcagtt cactacccgg caattgatac aacttttcgt
    14821 tttcctgcat caacggcagc agcgtacggc aaattgagcg gatacagtga acgcgcatca
    14881 ttaagccgta atgacgactc ataaactcaa gattatcaat gcgcaggcag caaaaactct
    14941 tgcccgcctc ttgctccggc gcttgttcca acgcacgaaa gttgggtagg agcgttaacg
    15001 gatctgtcag cgcctgcaaa tgccagcggc gattaagcca ttcactgcga tgataaatgc
    15061 gcaccatata gagcaggcaa acgctaaagg aaatcagcac cgcgagaata aatgccagcg
    15121 aatattcggt ttccacccct tgcaaaaagt tctggttgta attcagaagg caaagcgttg
    15181 aaacagccca ggtgagattt aaaaacggat agcgaagatt accgaccccg agggtaaaga
    15241 tgatgaagaa aacgggtacc aggtaaccgg caataaagtc gttttcataa ggtgtgcaca
    15301 acagaagtag cagcacgctt aacgctgcca gccagcttaa ggtaaatgcg cgtttctctt
    15361 tgcccaacga cggagcgata tccctgcgcc acaatatctg cgcaaagtgg ggacttacaa
    15421 tcatgcgagt gagatagtag aaaagcatgt tgtaaatcag cactgcggtg aaaaggctta
    15481 gtaaatcaac gaccgtgaaa atggcatccg catcgccgaa aaaggtggat atctttagcg
    15541 ggaaatcaaa gaagctcccc acaagataca tgctgcattt gatgccgatc ggcgtcacca
    15601 aaccaagcca gaacagacgt tgccagacat atcggctggt caatccataa cgccaacgcg
    15661 tccccagctg ccagcgtaat attgcgcagg cacacaacac gacaaacgtc tggctgccga
    15721 gcatgaccgc agtctgtaaa aaagagagat gaaagttcca cagattggtg caaaacatcc
    15781 cgcataaaac gggaacgaca ccgcgccagc caaagataaa caagattgac agcattacgc
    15841 acagtggcat ccacgccaga aagatataac tggaatggat aatggcaagt ggcgaaataa
    15901 aacgggatag ctgaataagt accacggtga gcgtaaacgc tagtgtgaat atcttgatat
    15961 tttttatcag gttatgctcc acaaacatga gtacactact ctcactgatc ggggttttca
    16021 ggcacacaaa taataagttt attcgcatcg cgaagcaaac aggggccagc ctgttgccga
    16081 tttttcatcc aatgatgctt tcaccaggcg gcgctaaccc ggacgtagac tgtattacaa
    16141 aagcggcaaa aagcagagac aaaaaacccc cgctttgcag cgagggttgg aaatttggtg
    16201 gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
    16261 gctatagccc cacgatgcgt ttacgtacca aatttgctgg gtgcaaaatt tggtggagct
    16321 aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca gctgagctat
    16381 agccccgtca cgtaaagctt gtcgagttga cgggcggcat catatgaatt ccgcccgaat
    16441 gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga aaaatcaggc aaatgaacaa
    16501 ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt aaagtggtgt
    16561 tataaaatga actactaata gacccacata cattcaggga attgttatgt tcaaggagag
    16621 gatgacacca gatgaacttg ccagactgac cggctatagc cgccagacca ttaataaatg
    16681 ggtacgcaag gaaggctgga caacgtcacc aaaacctggc gtccagggtg gcaaagcccg
    16741 actggttcac gtcaatgaac aggttcgtga atatattcgc aatgctgaac gtccagaagg
    16801 tcagggagaa gcgcctgccc tttctggtga tgcgccgctt gaagtattgc tggtgacact
    16861 ggcgaaagag atgacgccag ttgaacaaaa acagtttaca tccttgcttc tgcgggaagg
    16921 gattatcgga ttgttacaac gcttagggat tcgcgatagc aaataatatg aaaagattac
    16981 gcaataaaat gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa caaactgtta
    17041 accgttggat cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg aaaggcggtc
    17101 gtgccagact tattctggtc gatacgcaag tttgcgagtt tattcagaac acgccagcct
    17161 tccataacac accaatgttg atggaagctg aagagagaat tgctgaatac gctcctggag
    17221 cacgcgcccc ggcgtatcga caaatcatca acgcgataga taatatgaca gacattgagc
    17281 aggaaaaagt cgcgcaattt ttgtcacgtg aaggaattcg caatttcctc gcccgtctcg
    17341 atattgacga atcagcataa ataaaaaacg gcaggatatt atctcctgcc gtttatcttt
    17401 ttacacgcta attactgctg attttcgcgt tcagcaataa aatccagcgc tttgttgata
    17461 cgctcgatac tgcgggtctt accaattgcg tgaacggtaa catccagtgc tggagactgc
    17521 cccgcaccgg ttacggcgac acgcagcggc ataccaactt tacccatacc cacttccagc
    17581 tcatccgccg tcgcctgaat agcgtgatga acgttttcag cggtccagtc agtaatcgcg
    17641 gccagtttgt cacgaaccac ttccagcggc tgacgcgcta ccggacgcag atgttttttc
    17701 gccgcatcgg catcgaactc agcaaaatct tcgtagaaat aacggcagct ctgtgccatc
    17761 tctttcagcg tcttgcagcg ttcgcccagc agtttcacca gatcagccag ctgcgggccg
    17821 ttacgggtat cgatattttc ctgctcaatg tgccattgta agtgagtagc aacatactcc
    17881 ggcggcagcg cgttaatgta gtgatggttc agccacagca gcttgtcggt gttgaacgca
    17941 ctggcagatt tgctgacggc attcaaagtg aagtatttga tcatctcttc acgagtgaag
    18001 atttcctgat cgccgtggga ccagcccaga cgcaccagat agttcagcag tgcttctggc
    18061 aaataaccgt catcacgata ctgcattacg ctgactgccc cgtgacgttt ggacagtttt
    18121 ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa ccggcaccgg cgctttcagg
    18181 gccttaagaa tgttgatctg gcgcggcgtg ttgttgatat ggtcttcgcc acggataacg
    18241 tgggtgattt ccatatccca gtcatcgaca accacacaga agttataggt tggggaacca
    18301 tcggtacggc ggataataag atcgtccagt tcctggttgc tgaactcgat cggaccacgg
    18361 atctgatcgt caaaaacaac agaaccttcc tgcgggttag caaaacgtac aacacacggt
    18421 tcatcatcag catgatgctc atggctgtgg cggcagcgac cgtcataacg cggcttctca
    18481 cctttcgcca tttgctcttc gcgcagcgct tccaggcgct ctttagagca atagcattta
    18541 taagcagtgc cctcttccag catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg
    18601 gtctggtagt acggaccttc atcccactcc aggctcagcc agttcatgcc atccataatg
    18661 gcttcgatag cttccggcgt ggaacgctca agatcggtgt cttcaatacg cagcacgaac
    18721 tcaccgccgt ggttacgtgc aaaaagccag gagtaaagag cagtacgcgc gccgccaacg
    18781 tgcagatagc ctgttgggct tggcgcgaag cgagttttga ttttcatgaa atggccttac
    18841 gtttagaaag atgccgacaa ccggcaaatc ctggaaaaat tgagtgggcg atattctatc
    18901 actcccgcct gattcctcaa tgtagttcgg gtttctacct tacgcattat tgttttcgtt
    18961 gacaaattgc gcaacacggt ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa
    19021 caatttcttt agaaaatgcg ttgactcatt ttgaactctc cctataatac gactccacac
    19081 agcgggggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    19141 cgatcccgtc atcacccacc aactacttta tgtagtctcc gccgtgtagc aagaaattga
    19201 gaagtgggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    19261 cgatcccgtc atcacccacc acttcgggtc gttagctcag ttggtagagc agttgacttt
    19321 taatcaattg gtcgcaggtt cgaatcctgc acgacccacc aatgtaaaaa agcgccctaa
    19381 aggcgctttt ttgctatctg cgatttgtaa gattaatttt tcttctcaag ttccttgttc
    19441 ttctaccgat attacctcgt acagtgacga ggactgaaca atggccacta tctcttcaac
    19501 ttcaattcct tccatacaaa cacagagtag taacagggca tcacaaggta gcgatgttgc
    19561 ttcacaaatc gcgcggataa gtcagcaaat catcaaactc actcaacaaa ttaaagagat
    19621 tgtagacact agcggcagtg ctgaagacaa gcaaaaacag gccgaattga ttcaacagca
    19681 aatcacccta ctggaaacac aactggcgca gttgcagaaa cagcaggcag aaaaggcgca
    19741 ggagaaagag cagcgtttgt ctctgaatgt atcccttcta aatcctgttg aaaaaacgac
    19801 tcatatcgat atctatatct gatacaacgg atccccttcc cgaccagcca ttagcgcatt
    19861 gccctggtct cgtaccacgt cccataacgc ttcagcggcg gtggaaagtg aacggttctt
    19921 cctgcgcacc agcatcaact ggcgttcaac caccggcgta atgcgcttaa ccaccagtgg
    19981 gctaccttca ggcaatggta gcgccagtgc aggaagaata ctgatgccga tgcctgcggc
    20041 taccatcggg aacagcgtcg ccggatgtcc aatctcctgc acaatattcg cctgaatacc
    20101 attgcgcgcc agcgctgcgt caatcagcgg tcggctgcct gacgcgtaat cctgcaacac
    20161 caatttcgct ccctgtaatg cctgccaggg tacataatct tccacggcca aagcgctatc
    20221 gcgatggcaa agcagaaaga aaggctcgga aagaatcgct tcacattgca gatcgcccac
    20281 tgggcctgga tcgatgacga tgccaaagtc gacatctccc tggcgaatac tttccatcac
    20341 ccactgctgc ggacgatcat gcaggacaaa ctgaatatct ggatagcggc gatggctttc
    20401 agcaatgcat tgcggaataa gatgcgcgga aatggtctgg ctggcagcgc cccgaacttt
    20461 tccgctcagt tgttgcccca tacgcccggt atcgcgcaac gtgctgttaa gttcatccag
    20521 cagtcgctca agacgcaaag ccaactgctg ccctgcatct gtaagcacca cttcacgcgt
    20581 ggttctgtcc agcaggcgaa caccagtatg attttccagc tccttcacac tgtgacttac
    20641 tgccgactgg ctcaagccga tacgctctcc tgcacgacta aaacttttct cctgcgctac
    20701 tgtgacgaaa acctttaatt gttttaaaga ataattcatc tgttttcttc atgaatggat
    20761 gcaataaatc aattttattt ctcaaactgg aagaagcaca atagaaccat cgatcatctg
    20821 gagtctttat gaaacttttt cgtatcctcg atcctttcac cttaaccctg atcacggtgg
    20881 tgttgctggc ctctttcttt ccggccagag gcgatttcgt ccccttcttt gaaaatctga
    20941 ccaccgcagc tattgccctg ctgttcttta tgcacggcgc gaagttgtcg cgtgaggcga
    21001 ttattgctga cggtggtcac tggcgactgc atttgtgggt aatgtgcagc accttcgtgc
    21061 tgtttccgat tctgggtgta ctgtttgcct ggtggaaacc ggtaaatgtc gacccgatgc
    21121 tctactccgg ttttctctac ttgtgcattc tcccggctac cgtgcagtct gcaatcgcct
    21181 tcacgtcaat ggcgggcggt aacgtcgcgg cggcggtttg ttctgcgtca gcatccagcc
    21241 tgctggggat tttcctttca ccattgctgg ttggtctggt gatgaatgtt cacggtgcaa
    21301 ggggcagcct tgagcaggtc ggtaaaatta tgctgcaact gctgctgccg tttgtgttgg
    21361 ggcatctttc ccggccgtgg attggtgact gggtgtcgcg caataaaaaa tggattgcga
    21421 aaactgacca gacgtccatt ctgttggtgg tttatacagc gttcagcgaa gccgtcgtta
    21481 atggtatctg gcataaagtt ggctggggat cattgctgtt tatcgtggtg gtcagctgcg
    21541 ttcttctggc tatcgtgatt gtagttaacg tctttatggc acgccgactg ggcttcaata
    21601 aggcagatga aattactatc gtcttttgtg gttcgaaaaa gagtctggca aatggcatcc
    21661 cgatggcaaa cattctgttc cccacatcgg tgatcggtat gatggtgctg cccctgatga
    21721 ttttccatca gatccaattg atggtctgtg cggtgctggc gcgtcgatac aaacgccaga
    21781 ccgaacagtt acaggcgcag caggaaagca gcgccgataa agcttaaagc ggacgcttca
    21841 ggggctggac cagttgcgtc agcccctcgg ttttaatcag cagcgtgatt tgcattagct
    21901 caccgagctt ccctgccggg aactcatctt tgcgggcaaa ccacagcaga tactcctccg
    21961 gcaggtcaat taagcgacgc cctttgtatt tgccaaacgg cattatcgta ttggctattt
    22021 caatcagctg ctctttttcc atctcagcta cccagcaaac gcagcatttc cgcttcgtcg
    22081 atgacttcaa tgcccagttc ctgcgccttc gccagtttag atcctgcagc ttcacccgct
    22141 atcaccagat cggttttctt cgacacgctg cccgcgactt tcgcgcccag ttcgaccagt
    22201 cgagctttag cgtcatcacg cgacatctgg cttaagctgc ccgtaagcac cacggtttta
    22261 ccagcaaacg ggctgtcaat ctcttccgcg ttgataacga tcggcgcagg ccagtgaaca
    22321 ccttccgcca acagctcgct gatgacattg cggttgcttt cttcggcaaa gaagttgtga
    22381 acgtgggatg caacgacaat gccaacatca ggcacctttt gcagctcttc aatcgaagcg
    22441 gcttccagcg cttccagcgt gccgaaatat gccgccagac ctgctgcggt ggcctcgccg
    22501 acttcacgga tgccaagtgc atagaggaag cgagcaaagg tggtttcttt cgctttttcc
    22561 agcgcgttaa ccacgttttg tgccaatttt ggccccatac gctccagtcc ggtcagtttg
    22621 cctgcggtga gtttgaacag atctgccgga gtatggacat attctttttc aaccagctga
    22681 tcgatgattt tgtcgcccat tccgtcaaca tccatcgcac ggcgggaaac aaagtgtttc
    22741 agcgactctt tacgctgcgc accgcaaatc aggccaccgg tacagcgggc aaccgcttca
    22801 ccttccacac gctcaacgtc agaaccacat accggacaat gcgtcgggaa tacaacctca
    22861 cgggtatctt ccgggcgttc agaaagcacg acgttaacca cctgcgggat cacgtcgcca
    22921 gcgcggcgaa tcaccacttt atcgccaatg cgtaaaccaa gacgttcgat ttcatccgca
    22981 ttgtgtaagg ttgcgttact caccagcacg ccagcaacat ggacaggttc cagacgcgca
    23041 acaggcgtaa tcgcgccagt acgcccaacc tgaaactcga cgtcacgcac aaaggtcatc
    23101 tgctcctgcg ccgggaattt aaacgctacc gcccagcgcg gggcacgcgc gacaaagcca
    23161 agctgctcct gctgttccag tgagttgacc ttaatcacca cgccgtcgat atcaaagccc
    23221 agcgtcgggc ggtcttcttc cactttgtga tagaacgcca gcacttcttc cgccgattca
    23281 caaagcgtta cccgatcgct gaccggcaac ccccactttt taaattgcag taaacggcca
    23341 agatgagtat ccggcagctc gccaccttcc agaacaccaa cgccatagca gaaaaaagtg
    23401 agcggtcgct tcgctgtaat acgcggatca agctgacgca gtgaaccagc tgccgcatta
    23461 cgtgggttag caaaaacttt cccgcccgtg cgtcgcgcat cttcgttaat cttttcgaac
    23521 cccgcctgcg gcaggaacac ttcaccacgc acttccagac gcgccgggat attctctccg
    23581 tgcagcttca gcggaatggc gcgaatagta cgcacattag acgtgatatc ttccccggtg
    23641 gtgccatcgc cacgggtcgc ggcactgact aaaacgccat tttcatacag aatactgacg
    23701 gcaaggccat ccagcttcag ctcacagcac caggtgactt tctcgttgtt tttcagacgg
    23761 tcctgcacac gtttgttgaa agcaagaaag ctttcttcat caaaaacgtt atccagtgac
    23821 agcattggta cttcatggcg tatctggctg aaagctgcca gcggcgcagc acctacacgt
    23881 tgggtaggcg aatcaggcgt aatcagttct ggatgtttgg tttccagctc gcgcagttcg
    23941 cgcatcagcc tgtcgtattc agcgtcggga atttccggcg catccatcac atgataaaga
    24001 tattcatgat ggcaaagcgt cgttcgcagt tctgtcagtt gttgttcgat tgattccata
    24061 tcgcaccatc aatgctaaaa acccccgaca agcgggggtt cgaagaggag ttaatttgcc
    24121 ttaagtgtat caggcgttgg cgtctttgac ttcgcggatg atgtcctggt actcgcgcaa
    24181 tttctgcgga gtcatcatac ggcgctgatc gtcaagcacg acaccgccca cttcatcggc
    24241 aatatgctgc gcagattgca gcatcagctt gaagttctgc agctcgtcac cgtaagacgg
    24301 tacctgcata aagatagtga cacccggagt agtgaaatcc ttcatttcag gatcaaaggt
    24361 tcccggtttc accatattcg ccaggctgaa taacgccggg ccgctgccat ccgggctaag
    24421 atgacgatgg taaatattca tatcgccaaa aatgaagccc gcttgttgaa tgctgttaag
    24481 aagcagttca ccgtttagct cgctaccgtg atgcgccgcg acgttcatga taatcaccgc
    24541 ttctttgcgc ttcggtttat ccataactgg agccggttcc gctacaggct caggctgtgg
    24601 tgccgctacg ggttctgcag gctggaaagc ctgttgtgcc ggttgcggtg ctgaatgcac
    24661 aggctgcggc gctggcgcga cctgtggcga aactggctgc tgcaacggct gttcaggctg
    24721 cggctgatag gcaggctgct gcaccggctg cgctggacgc ggagcatgtt gcggcggtac
    24781 ctgcgcttca ggcggctgct ggaccggttg acgcggctgc gcagacgcat aaggcggttg
    24841 gtactggtgt tgcggcgacg gacgagcagc ctcatgctcc tgagcgttag ccggggcatg
    24901 attcacgcgg tgaacacgaa cctcaccaac gccctcatca tcttcgacat cctcgtcata
    24961 agaatcgtcg tcacgttttg acttcattcg ttttaatggc cgatcgcgga acatagaaga
    25021 tcgttcttta cggctggtcc agaaaccatg taccagtaaa gcgattatgg cgatcgcgcc
    25081 aacaatgatt aatatcagac gcaaatcctg catcattata ttctctgttg ttctaacacc
    25141 ttgccaccac ggcaaacatt tactcactaa gagtatttgc cgattacctc aagtgcaagt
    25201 gcactattaa ctttcacagc acaaagatag atgaaatcgt gctttttgct gttttttcga
    25261 acatatccta actgtccatt gcgcaattac ccggtaaaat acgcagaatt ttcctgggat
    25321 tggtcaaaag gagctcatcc tgattatggt ttcatcattc acatctgccc cacgcagcgg
    25381 tttttactat tttgcgcaag gctggaagct cgtctcgcaa cctgggattc ggcgtttcgt
    25441 tattttaccg ctgctggtca atattttgtt gatggggggc gcattctggt ggctctttac
    25501 acagctcgat gtctggatcc cgactttcat gagttacgtt ccggactggc tacaatggct
    25561 gagttatctg ttgtggcctc tggctgtcat ctctgtgctg ttagtgtttg gctatttctt
    25621 ctccacgatt gctaactgga ttgccgctcc gtttaacggt ttattggctg aacaactgga
    25681 agcacgattg actggcgcta caccgccaga taccgggatt ttcggtatca tgaaagatgt
    25741 gccgcgaatc atgaaacgcg aatggcaaaa atttgcctgg tatctgccgc gcgcaattgt
    25801 attactaatt ctttacttca tccctggtat tgggcaaacc gtcgcgccgg tactgtggtt
    25861 cctgtttagc gcctggatgt tagccatcca gtattgcgat taccccttcg ataaccacaa
    25921 agtgccgttt aaagagatgc gcaccgccct gcgcacacgc aaaatcacca atatgcagtt
    25981 tggtgcttta accagcctgt ttacgatgat cccgctgctt aatctgttca tcatgcccgt
    26041 tgccgtttgt ggcgcaacgg cgatgtgggt cgattgctat cgcgataaac acgcgatgtg
    26101 gcggtaacaa tctaccggtt attttgtaaa ccgtttgtgt gaaacagggg tggcttatgc
    26161 cgccccttat tccatcttgc atgtcattat ttcccttctg tatatagata tgctaaatcc
    26221 ttacttccgc atattctctg agcgggtatg ctacctgttg tatcccaatt tcatacagtt
    26281 aaggacaggc catgagtaag atttttgaag ataactcgct gactatcggt cacacgccgc
    26341 tggttcgcct gaatcgcatc ggtaacggac gcattctggc gaaggtggaa tctcgtaacc
    26401 ccagcttcag cgttaagtgc cgtatcggtg ccaacatgat ttgggatgcc gaaaagcgcg
    26461 gcgtgctgaa accaggcgtt gaactggttg aaccgaccag cggtaatacc gggattgcac
    26521 tggcctatgt agctgccgct cgcggttaca aactcaccct gaccatgcca gaaaccatga
    26581 gtattgaacg ccgcaagctg ctgaaagcgt taggtgcaaa cctggtgctg acggaaggtg
    26641 ctaaaggcat gaaaggcgca attcaaaaag cagaagaaat tgtcgccagc aatccagaga
    26701 aatacctgct gctgcaacaa ttcagcaatc cggcaaaccc tgaaattcac gaaaaaacca
    26761 ccggtccgga gatctgggaa gataccgacg gtcaggttga tgtatttatt gctggcgttg
    26821 ggactggcgg tacgctgact ggcgtcagcc gctacattaa aggcaccaaa ggcaagaccg
    26881 atcttatctc tgtcgccgtt gagccaaccg attctccagt tatcgcccag gtgctggcag
    26941 gtgaagagat taaacctggc ccgcataaaa ttcagggtat tggcgctggt tttatcccgg
    27001 ctaacctcga tctcaagctg gtcgataaag tcattggcat caccaatgaa gaagccattt
    27061 ctaccgcccg tcgtctaatg gaagaagaag gtattcttgc aggtatctct tctggagcag
    27121 ctgttgccgc ggcgttgaaa ctacaagaag atgaaagctt taccaacaag aatattgtgg
    27181 ttattctacc atcatcgggt gagcgttatt taagcaccgc attgtttgcc gatctcttca
    27241 ctgagaaaga attgcaacag taatgccagc ttgttaaaaa tgcgtaaaaa agcacctttt
    27301 taggtgcttt tttgtggcct gcttcaaact ttcgcccctc ctggcattga ttcagcctgt
    27361 cggaactggt atttaaccag actaattatt ttgatgcgcg aaattaatcg ttacaggaaa
    27421 agccaaagct gaatcgattt tatgatttgg ttcaattctt cctttagcgg cataatgttt
    27481 aatgacgtac gaaacgtcag cggtcaacac ccgccagcaa tggactgtat tgcgctcttc
    27541 gtgcgtcgcg tctgttaaaa actggcgcta acaatacagg ctaaagtcga accgccaggc
    27601 tagactttag ttccacaaca ctaaacctat aagttgggga aatacaatgt tccagcaaga
    27661 agttaccatt accgctccga acggtctgca cacccgccct gctgcccagt ttgtaaaaga
    27721 agctaagggc ttcacttctg aaattactgt gacttccaac ggcaaaagcg ccagcgcgaa
    27781 aagcctgttt aaactgcaga ctctgggcct gactcaaggt accgttgtga ctatctccgc
    27841 agaaggcgaa gacgagcaga aagcggttga acatctggtt aaactgatgg cggaactcga
    27901 gtaatttccc gggttctttt aaaaatcagt cacaagtaag gtagggttat gatttcaggc
    27961 attttagcat ccccgggtat cgctttcggt aaagctctgc ttctgaaaga agacgaaatt
    28021 gtcattgacc ggaaaaaaat ttctgccgac caggttgatc aggaagttga acgttttctg
    28081 agcggtcgtg ccaaggcatc agcccagctg gaaacgatca aaacgaaagc tggtgaaacg
    28141 ttcggtgaag aaaaagaagc catctttgaa gggcatatta tgctgctcga agatgaggag
    28201 ctggagcagg aaatcatagc cctgattaaa gataaacaca tgacagctga cgcagctgct
    28261 catgaagtta tcgaaggtca ggcttctgcc ctggaagagc tggatgatga atacctgaaa
    28321 gaacgtgcgg ctgacgtacg tgatatcggt aagcgcctgc tgcgcaacat cctgggcctg
    28381 aagattatcg acctgagcgc cattcaggat gaagtcattc tggttgccgc tgacctgacg
    28441 ccgtccgaaa ccgcacagct gaacctgaag aaggtgctgg gtttcatcac cgacgcgggt
    28501 ggccgtactt cccacacctc tatcatggcg cgttctctgg aactgcctgc tatcgtgggt
    28561 accggtagcg tcacctctca ggtgaaaaat gacgactatc tgattctgga tgccgtaaat
    28621 aatcaggttt acgtcaatcc aaccaacgaa gttattgata aaatgcgcgc tgttcaggag
    28681 caagtggctt ctgaaaaagc agagcttgct aaactgaaag atctgccagc tattacgctg
    28741 gacggtcacc aggtagaagt atgcgctaac attggtacgg ttcgtgacgt tgaaggtgca
    28801 gagcgtaacg gcgctgaagg cgttggtctg tatcgtactg agttcctgtt catggaccgc
    28861 gacgcgctgc ccactgaaga agaacagttt gctgcttaca aagcagtggc tgaagcgtgt
    28921 ggctcgcagg cggttatcgt tcgtaccatg gacatcggcg gcgacaaaga gctgccatac
    28981 atgaacttcc cgaaagaaga gaacccgttc ctcggctggc gcgctatccg tatcgcgatg
    29041 gatcgtaaag agatcctgcg cgatcagctc cgcgctatcc tgcgtgcctc ggctttcggt
    29101 aaattgcgca ttatgttccc gatgatcatc tctgttgaag aagtgcgtgc actgcgcaaa
    29161 gagatcgaaa tctacaaaca ggaactgcgc gacgaaggta aagcgtttga cgagtcaatt
    29221 gaaatcggcg taatggtgga aacaccggct gccgcaacaa ttgcacgtca tttagccaaa
    29281 gaagttgatt tctttagtat cggcaccaat gatttaacgc agtacactct ggcagttgac
    29341 cgtggtaatg atatgatttc acacctttac cagccaatgt caccgtccgt gctgaacttg
    29401 atcaagcaag ttattgatgc ttctcatgct gaaggcaaat ggactggcat gtgtggtgag
    29461 cttgctggcg atgaacgtgc tacacttctg ttgctgggga tgggtctgga cgaattctct
    29521 atgagcgcca tttctatccc gcgcattaag aagattatcc gtaacacgaa cttcgaagat
    29581 gcgaaggtgt tagcagagca ggctcttgct caaccgacaa cggacgagtt aatgacgctg
    29641 gttaacaagt tcattgaaga aaaaacaatc tgctaatcca cgagatgcgg cccaatttac
    29701 tgcttaggag aagatcatgg ggttgttcga taaactgaaa tctctggttt ccgacgacaa
    29761 aaaggatacc ggaactattg agatcattgc tccgctctct ggcgagatcg tcaatatcga
    29821 agacgtgccg gatgtcgttt ttgcggaaaa aatcgttggt gatggtattg ctatcaaacc
    29881 aacgggtaac aaaatggtcg cgcctgtaga cggtaccatt ggtaaaatct ttgaaaccaa
    29941 tcacgcgttc tctatcgaat ctgatagcgg cgttgaactg ttcgtccact tcggtatcga
    30001 caccgttgaa ctgaaaggcg aaggcttcaa gcgtattgct gaagaaggtc agcgcgtgaa
    30061 agttggcgat actgtcattg aatttgatct gccgctgctg gaagagaaag ccaagtctac
    30121 cctgactccg gttgttatct ccaacatgga cgaaatcaaa gaactgatca aactgtccgg
    30181 tagcgtaacc gtgggtgaaa ccccggttat ccgcatcaag aagtaattct tgccgcagtg
    30241 aaaaatggcg cccatcgg
//
LOCUS       DBKEKI010000040        29706 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.040, whole genome shotgun sequence.
ACCESSION   DBKEKI010000040 DBKEKI010000000
VERSION     DBKEKI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.040"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1791)
                     /gene="speA"
                     /locus_tag="ACUQPX_001753"
     CDS             complement(<1..1791)
                     /gene="speA"
                     /locus_tag="ACUQPX_001753"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HHV9056485.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEV"
     gene            complement(1800..1931)
                     /gene="yqgB"
                     /locus_tag="ACUQPX_001754"
     CDS             complement(1800..1931)
                     /gene="yqgB"
                     /locus_tag="ACUQPX_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HHV9056486.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(2025..2168)
                     /gene="yqgG"
                     /locus_tag="ACUQPX_001755"
     CDS             complement(2025..2168)
                     /gene="yqgG"
                     /locus_tag="ACUQPX_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HHV9056487.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2279..2530)
                     /gene="yqgD"
                     /locus_tag="ACUQPX_001756"
     CDS             complement(2279..2530)
                     /gene="yqgD"
                     /locus_tag="ACUQPX_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HHV9056488.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2586..3740
                     /gene="metK"
                     /locus_tag="ACUQPX_001757"
     CDS             2586..3740
                     /gene="metK"
                     /locus_tag="ACUQPX_001757"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HHV9056489.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4177..5571
                     /gene="galP"
                     /locus_tag="ACUQPX_001758"
     CDS             4177..5571
                     /gene="galP"
                     /locus_tag="ACUQPX_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HHV9056490.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5648..6145
                     /locus_tag="ACUQPX_001759"
     CDS             5648..6145
                     /locus_tag="ACUQPX_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HHV9056491.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6240..6947
                     /gene="endA"
                     /locus_tag="ACUQPX_001760"
     CDS             6240..6947
                     /gene="endA"
                     /locus_tag="ACUQPX_001760"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HHV9056492.1"
                     /translation="MYRYLSIAAVVLSAAFSGTTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTHSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7027..7758
                     /gene="rsmE"
                     /locus_tag="ACUQPX_001761"
     CDS             7027..7758
                     /gene="rsmE"
                     /locus_tag="ACUQPX_001761"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HHV9056493.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            7771..8721
                     /gene="gshB"
                     /locus_tag="ACUQPX_001762"
     CDS             7771..8721
                     /gene="gshB"
                     /locus_tag="ACUQPX_001762"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HHV9056494.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            8830..9393
                     /locus_tag="ACUQPX_001763"
     CDS             8830..9393
                     /locus_tag="ACUQPX_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HHV9056495.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9393..9809
                     /gene="ruvX"
                     /locus_tag="ACUQPX_001764"
     CDS             9393..9809
                     /gene="ruvX"
                     /locus_tag="ACUQPX_001764"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HHV9056496.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(9985..10965)
                     /locus_tag="ACUQPX_001765"
     CDS             complement(9985..10965)
                     /locus_tag="ACUQPX_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HHV9056497.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            10983..11687
                     /gene="yggS"
                     /locus_tag="ACUQPX_001766"
     CDS             10983..11687
                     /gene="yggS"
                     /locus_tag="ACUQPX_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HHV9056498.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11705..12271
                     /gene="yggT"
                     /locus_tag="ACUQPX_001767"
     CDS             11705..12271
                     /gene="yggT"
                     /locus_tag="ACUQPX_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HHV9056499.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQSTGNMLLPGLWMAL"
     gene            12268..12558
                     /gene="yggU"
                     /locus_tag="ACUQPX_001768"
     CDS             12268..12558
                     /gene="yggU"
                     /locus_tag="ACUQPX_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HHV9056500.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12566..13159
                     /locus_tag="ACUQPX_001769"
     CDS             12566..13159
                     /locus_tag="ACUQPX_001769"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HHV9056501.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13152..14288
                     /gene="hemW"
                     /locus_tag="ACUQPX_001770"
     CDS             13152..14288
                     /gene="hemW"
                     /locus_tag="ACUQPX_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HHV9056502.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14602..15565
                     /locus_tag="ACUQPX_001771"
                     /pseudo
     CDS             14602..15565
                     /locus_tag="ACUQPX_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15610..16113
                     /locus_tag="ACUQPX_001772"
     CDS             15610..16113
                     /locus_tag="ACUQPX_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HHV9056503.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            16113..17414
                     /locus_tag="ACUQPX_001773"
     CDS             16113..17414
                     /locus_tag="ACUQPX_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HHV9056504.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(17470..18477)
                     /locus_tag="ACUQPX_001774"
     CDS             complement(17470..18477)
                     /locus_tag="ACUQPX_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HHV9056505.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(18594..19640)
                     /gene="ansB"
                     /locus_tag="ACUQPX_001775"
     CDS             complement(18594..19640)
                     /gene="ansB"
                     /locus_tag="ACUQPX_001775"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HHV9056506.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(19816..20535)
                     /locus_tag="ACUQPX_001776"
     CDS             complement(19816..20535)
                     /locus_tag="ACUQPX_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HHV9056507.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(20719..21045)
                     /locus_tag="ACUQPX_001777"
     CDS             complement(20719..21045)
                     /locus_tag="ACUQPX_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HHV9056508.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGRICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            complement(21045..21764)
                     /gene="trmB"
                     /locus_tag="ACUQPX_001778"
     CDS             complement(21045..21764)
                     /gene="trmB"
                     /locus_tag="ACUQPX_001778"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HHV9056509.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            21925..22977
                     /gene="mutY"
                     /locus_tag="ACUQPX_001779"
     CDS             21925..22977
                     /gene="mutY"
                     /locus_tag="ACUQPX_001779"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HHV9056510.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTSCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            23005..23280
                     /locus_tag="ACUQPX_001780"
     CDS             23005..23280
                     /locus_tag="ACUQPX_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HHV9056511.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            23345..24424
                     /gene="mltC"
                     /locus_tag="ACUQPX_001781"
     CDS             23345..24424
                     /gene="mltC"
                     /locus_tag="ACUQPX_001781"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HHV9056512.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            24626..25882
                     /gene="nupG"
                     /locus_tag="ACUQPX_001782"
     CDS             24626..25882
                     /gene="nupG"
                     /locus_tag="ACUQPX_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HHV9056513.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(25931..28066)
                     /gene="speC"
                     /locus_tag="ACUQPX_001783"
     CDS             complement(25931..28066)
                     /gene="speC"
                     /locus_tag="ACUQPX_001783"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HHV9056514.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACSGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFVPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LSGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(28228..28311)
                     /gene="yqgH"
                     /locus_tag="ACUQPX_001784"
     CDS             complement(28228..28311)
                     /gene="yqgH"
                     /locus_tag="ACUQPX_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HHV9056515.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            28464..29162
                     /locus_tag="ACUQPX_001785"
     CDS             28464..29162
                     /locus_tag="ACUQPX_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HHV9056516.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTA"
     gene            29277..29352
                     /locus_tag="ACUQPX_001786"
     tRNA            29277..29352
                     /locus_tag="ACUQPX_001786"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29310..29312,aa:Phe,seq:gaa)
BASE COUNT         7191 a         7692 c         7333 g         7490 t
ORIGIN      
        1 tacttctacg ctaccgtcag ggaacacgaa cacgtcaact gcttcggtat caccgaacag
       61 gttgtgcata ttgccaagga tctcctgata tgcaccgacc ataaagaaac cgagcatcgg
      121 cggattctct ggatcgtact ccggcattgg cattgtcgtg gcaataccat caccatcaat
      181 atagtggtcg atagcaccgt cagagtcaca ggtaatatcc agcagcacag cgcgacgttc
      241 cggcacttga tccagccctt ccagcggtag aaccgggaac aactggtcga tcccccatgc
      301 gtccggcatt gactggaaca gcgagaaatt gacgtacatt ttgtccgcca tacgttcctg
      361 cagttcgtcg attatcggac gatgcgcacg gttttgcgga tccagttgct tctgcacttc
      421 atggcacatg ctcagataga gttgttctgc ccatgcacgt tcttgcaagc taaaggtgcc
      481 ggaagagtag ccgatatgaa tgtcgtgcag atccatctgg ctgtcgtgta accattcacg
      541 caaagaacgg cgagttcccg gttcgtgcat ttcctgccag gtttcccaca tgctttgcag
      601 cgcacgcggc gcatcttctg cgggcgcggt cggcaccgta tattcgttac gttccacgcc
      661 gatgatattc gacaccagca cggtatggtg cgcagtcacc gcacggcccg attcggtgat
      721 taccgtcgga tgcggcagac cattttcttc acacgcatcg ccaatcgccc agatgatgtt
      781 gttggcgtat tcattgaggc catagtttac ggagcagtca gactgcgaac gggtgccttc
      841 ataatccacg cccagaccgc cgccgacgtc gaagcactga atattgacgc ccagcttgtg
      901 cagttccaca tagaaacgcg cggattcacg aacgcctgtc gcgatatcgc gaatattcgc
      961 catctgcgaa ccgaggtgga agtgcagtag ttgcaggctg tcgagacgcc cggcttcacg
     1021 cagggtttca accagttgca gtacctgagt cgcagccagg ccgaacttcg atttttcccc
     1081 gccggaggac tgccatttac ccgagccctg cgaagccaga cgtgcacgca cgcccagacg
     1141 aggaacgaca ttcagacgtt ctgcttcatc cagcacaatg gcgatttctg acatcttctc
     1201 aatgaccaga tagaccttgt gccccatctt ctcgccaatt aatgccaggc ggatatattc
     1261 gcggtcttta taaccgttgc agacgatgac gctacgagtc atgccagcat gtgccagcac
     1321 tgccatcaac tcggctttgg aaccggcttc cagacccagc ggttcacccg aatgaatcag
     1381 ggactcgatc acgcggcggt gctggttaac tttgatcgga taaacaagga agtaatcgcc
     1441 gttatagccg taggattccc tcgcacgttt gaacgcggcg ttaatggaac gcaaacggtg
     1501 ctgcaggatc tgtgggaaac agaacagtgc aggcagacgc tgaccctgtg cttcacgagt
     1561 tttcactaac tgcgcgagat cgacgcgagc ttccgggacg tccgggtccg ggcacacgct
     1621 gatgtggccc agctcgttaa cgtcatagta gttattgccc caccaggcaa tattgtaagt
     1681 acgcagcatc ttgctggctt cctgggagct cattgcaacc tcctgcatgg agcgtagtac
     1741 accgtgttcg cccgctgacg aaggcaaacc catagacatg tcgtcagaca tagcgaacct
     1801 caaattattt tattaagtgt aaaacagtta acgactatcg cagcctggaa ctgcgataac
     1861 aacccataag cacatggatt ttccagcagt gtatgctgac gctccgactg cgcaaccggt
     1921 ttctttttca taacattatt aagcacataa ccgaacgtaa gtgtgacagt tcggcgaaac
     1981 cacgagaaaa ctcttgtttt tacaagagcg cccttgttca gtcctcagta actgtaacca
     2041 gctcttgaat cctgagaagc gccgagatgg gtataacatc ggcaggtatg caaagcagag
     2101 atgcagagtg cggggaacga atcttcacca gaacggtgag acaggttaag cagcagacaa
     2161 cggttcatta tttcgtatca cctccacggc cgcctgttaa gacgaaccca caagccaaaa
     2221 ctctgatttc aacccggctg gaagtggcaa cacgaaagaa acgtcgtgtg ctttttattt
     2281 aagccgcgcg ccgcgtttta taccccacaa tggcagaaaa ttgcaaaaga taaatacgca
     2341 gaatgccggc attgtcagga aaaatttcca gccacgtttt taacagaatg agacacgatt
     2401 caaaaaaaag tggaaatcgg gtgaagaatt gacctaaaat agccatccag atgttaatcc
     2461 atccataccg attaacactc agactgccag tgtttttaac ctgcagagtc gtggtaggat
     2521 ccgctaccac agaaaatcca cacaacagtt tgagctaacc aaattctctt taggtgatat
     2581 taaatatggc aaaacacctt tttacgtccg agtccgtctc tgaagggcat cctgacaaaa
     2641 ttgctgacca aatctctgat gccgttttag acgcgatcct cgaacaggat ccgaaagcac
     2701 gcgttgcttg cgaaacctac gtaaaaaccg gcatggtttt agttggcggc gaaatcacca
     2761 ccagcgcctg ggtagacatc gaagagatca cccgtaacac cgttcgcgaa attggctatg
     2821 tgcattccga catgggcttt gacgctaact cctgtgcggt tctgagcgct atcggcaaac
     2881 agtctcctga catcaaccag ggcgttgacc gtgccgatcc gctggaacag ggcgcgggtg
     2941 accagggtct gatgtttggc tacgcaacta atgaaaccga cgtgctgatg ccagcaccta
     3001 tcacctatgc acaccgtctg gtacagcgtc aggctgaagt gcgtaaaaac ggcactctgc
     3061 cgtggctgcg cccggacgcg aaaagccagg tgactttcca gtatgacgac ggcaaaatcg
     3121 ttggtattga tgctgtcgtg ctttccactc agcactctga agagatcgac cagaaatcgc
     3181 tgcaagaagc ggtaatggaa gagatcatca agccaattct gcccgctgaa tggctgactt
     3241 ctgccaccaa attcttcatc aacccgaccg gtcgtttcgt tatcggtggc ccaatgggtg
     3301 actgcggtct gactggtcgt aaaattatcg ttgataccta cggcggcatg gcgcgtcacg
     3361 gtggcggtgc attctctggt aaagatccat caaaagtgga ccgttccgca gcctacgcag
     3421 cacgttatgt cgcgaaaaac atcgttgctg ctggcctggc cgatcgttgt gaaattcagg
     3481 tttcctacgc aatcggcgtg gctgaaccga cctccatcat ggtagaaact ttcggtactg
     3541 agaaagtgcc ttctgaacaa ctgactctgc tggtacgtga gttcttcgac ctgcgcccat
     3601 acggtctgat tcagatgctg gatctgctgc acccgatcta caaagaaacc gcagcatacg
     3661 gtcactttgg tcgtgaacat ttcccgtggg aaaaaaccga caaagcgcag ctgctgcgcg
     3721 atgctgccgg tctgaagtaa tctttcttca cctgcgttca aaggccagcc tcgcgctggc
     3781 ctttttcttt tacctcaccg cggtggatag gcgttcacgc cgcatccggc aaaaaaactg
     3841 cccgcacaat aacatcattc ttcctgatca cgtttcacgg caaattacca tcacaactga
     3901 aaccgattac accaaccaca ccagcaaaat atttgtaata ttttcatatt attattcagc
     3961 tttcacagtt gttacatttc ttttcagtaa agtcttaatt gcagatgtca gcgtttaatc
     4021 tatgatgata taactcaatt attttcatgc acttaaatca taactaagat aaatgttagt
     4081 gtaagcgatt acactgatgt gatttgcttc acatcttttt acgtcgtact cacctatctt
     4141 aattcacaat aaaaaataac catattggag ggcatcatgc ctgacgctaa aaaacagggg
     4201 cggtcaaaca aggcaatgac gtttttcgtc tgcttccttg ccgctctggc gggattactc
     4261 tttggcctgg atatcggtgt aattgctggc gcactgccgt ttattgcaga tgaattccag
     4321 ataacttcgc acacgcaaga atgggtcgta agctccatga tgttcggtgc ggcagtcggt
     4381 gcggtgggca gcggctggct ctcctttaaa ctcgggcgca aaaagagcct gatgatcggc
     4441 gcaatcctgt ttgttgccgg ttcactgttc tctgcggctg cgccaaacgt tgaagtactg
     4501 attctttcgc gcgttctgct ggggctggcg gtgggtgtgg cctcttatac cgcaccactg
     4561 tacctgtcag aaattgcgcc ggaaaaaatt cgcggcagta tgatctcgat gtatcagttg
     4621 atgatcacta tcgggatcct cggtgcttat ctttctgata ccgccttcag ctacaccggt
     4681 gcatggcgct ggatgctggg tgtgattatc atcccggcaa ttttgctgct gattggtgtc
     4741 ttcttcctgc cagacagccc acgttggttt gccgccaaac gccgttttgt tgatgccgaa
     4801 cgcgtgctgc tacgcctgcg tgacaccagc gcggaagcga aacgcgaact ggatgaaatc
     4861 cgtgaaagtt tgcaggttaa acagagtggc tgggcgctgt ttaaagagaa tagcaacttc
     4921 cgccgtgcgg tgttccttgg cgtactgtta caggtaatgc agcaattcac cgggatgaac
     4981 gtcatcatgt attacgcgcc gaaaatcttc gaactggcgg gttataccaa caccaccgag
     5041 caaatgtggg ggacagtgat tgtcggcctg accaacgtac ttgccacctt tatcgcaatc
     5101 ggccttgttg accgctgggg acgtaaacca acgctaacgc tgggcttcct ggtgatggct
     5161 gctggcatgg gcgtactcgg tacaatgatg catatcggta tccactctcc gtcggcgcag
     5221 tatttcgcca tcgccatgct gctgatgttt attgtcggtt ttgccatgag tgccggtccg
     5281 ctgatttggg tactgtgctc cgaaattcag ccgctgaaag gccgcgattt tggcatcacc
     5341 tgctccaccg ccaccaactg gattgccaac atgatcgttg gcgcaacgtt cctgaccatg
     5401 ctcaacacgc tgggtaacgc caacaccttc tgggtgtatg cggctctgaa cgtactgttt
     5461 atcctgctga cattatggct ggtaccggaa accaaacacg tttcgctgga acatattgaa
     5521 cgtaatctga tgaaaggtcg taaactgcgc gaaatcggcg ctcacgatta atctcctcag
     5581 gcttcctccc gtcgcggagg aagccgcctc ttgcagtcat cttttcttcg ctctatcctc
     5641 tgccgctatg aaaacatccc gtctccctat cgccatccaa caggccgtta tgcgccgcct
     5701 gcgggaaaaa ctcgcccagg ccaacctgaa gctagggcgt aactacccgg agccaaaact
     5761 ctcttacacc cagcgcggaa cctccgccgg aacagcctgg ctggaaagct atgaaatccg
     5821 cctcaatccc gttttgctgt tggaaaacag tgaagctttt attgaagaag tggtaccgca
     5881 cgaactggca catttgctgg tatggaaaca tttcggccgc gtagcgccac atggcaaaga
     5941 gtggaagtgg atgatggaaa gcgtgctggg cgttcccgcc cgtcgtacgc atcagttcga
     6001 actgcaatcc gtgcgtcgca acaccttccc ctaccgctgt aagtgccagg aacatcagct
     6061 taccgtacgc cgccataatc gcgtagttcg tggcgaggcc atctatcgct gtgttcactg
     6121 cggtgaacag ctggttgcga aataaccacc tgaactatca ggaactttcc tgatctggct
     6181 gattgcatac caaaacaact ttcgctacgt tgctggctcg ttttaacacg gagtaagtga
     6241 tgtaccgtta tttgtctatt gctgcggtgg tactgagcgc agcattttcc ggcacgacgt
     6301 tggccgaagg tatcaatagt ttttctcagg cgaaagccgc ggcggtaaaa gtccacgctg
     6361 atgcgcccgg tacgttttat tgcggatgta aaattaactg gcagggcaaa aaaggcgttg
     6421 ttgatctgca atcgtgcggc tatcaggtgc gcaaaaatga aaaccgcgcc agccgcgtag
     6481 agtgggaaca cgtcgttccc gcctggcaat tcggtcacca gcgccagtgc tggcaggacg
     6541 gtggacgtaa aaactgcgct aaagatccgg tctatcgcaa gatggaaagc gatatgcata
     6601 acctgcagcc gtcagtcggc gaggtgaatg gcgatcgcgg caattttatg tacagccagt
     6661 ggaatggcgg cgaaggccag tacggtcaat gcgccatgaa ggtcgatttc aaagaaaaag
     6721 ctgccgaacc accagcgcgt gcacgcggtg ccattgcgcg cacctacttc tatatgcgcg
     6781 accaatacaa cctgacacac tctcgccagc aaacgcagct gttcaacgca tggaacaaga
     6841 tgtatccggt taccgactgg gagtgcgagc gcgatgaacg catcgcgaag gtgcagggca
     6901 atcataaccc gtatgtgcaa cgcgcttgcc aggcgcgaaa gagctaacct acactagcgg
     6961 gattcttttt gttaacccct accccacgcg tacaaccgcg tggggagacg acgcggattt
     7021 ttaactatgc gtatcccccg catttatcat cctgaaccac taaccagcca ttctcacatc
     7081 gcgctttgcg aagatgccgc caaccatatc gggcgcgtac tgcgcatggg gccggggcag
     7141 gcgttgcaat tgtttgacgg tagcaaccag gtctttgacg ccgaaattac cagcgccagc
     7201 aaaaaaagcg tggaagtgaa ggtgctggaa ggccagatcg acgatcgcga atctccgctg
     7261 catattcacc tcggtcaggt gatgtcgcgt ggtgaaaaaa tggaatttac tatccagaaa
     7321 tcgatcgaac tcggtgtaag cctcattacg ccactttttt ctgagcgctg cggcgttaaa
     7381 ctggatagtg aacgtctgaa caagaagctt cagcagtggc agaagattgc aattgctgcc
     7441 tgtgagcagt gtggtcgtaa ccgggtgccg gaaatccgtc cagcgatgga tctggaagcc
     7501 tggtgtgcag agcaggatga aggactgaaa ctgaatcttc acccgcgcgc cagtaacagc
     7561 atcaatacgt tgcctttacc ggttgaacgc gtccgcctgc tgattggccc ggaaggcggt
     7621 ttatcggcag atgaaattgc catgactgcc cgctatcaat ttactgatat cctgttggga
     7681 cctcgcgttt tgcgtacaga gacaactgcg ctcaccgcca ttaccgcgct acaagtacga
     7741 tttggcgatt tgggctaacg gagaagaata atgatcaagc tcggcatcgt gatggacccc
     7801 atcgcaaaca tcaacatcaa gaaagattcc agtttcgcta tgttgctgga agcacagcgt
     7861 cgtggttacg aacttcacta tatggagatg ggcgatctgt atctgatcaa tggcgaagcc
     7921 cgcgcccata cccgcacgct gaacgtgaag cagaactacg aagagtggtt ttcgttcgtc
     7981 ggtgaacagg atctgccgct ggccgatctc gatgtgatcc tgatgcgtaa agacccgccg
     8041 tttgataccg agtttatcta cgcgacctat attctggaac gtgccgaaga gaaagggacg
     8101 ctgatcgtta acaagccgca gagcctgcgc gactgtaacg agaaactgtt taccgcctgg
     8161 ttctctgact taacgccaga aacgctggtt acgcgcaata aagcgcagct aaaagcgttc
     8221 tgggagaaac acagcgacat cattcttaag ccgctggacg gtatgggcgg cgcgtcgatt
     8281 ttccgcgtga aagaaggcga tccaaacctc ggcgtgattg ccgaaaccct gactgagcat
     8341 ggcactcgct actgcatggc gcaaaattac ctgccagcca ttaaagatgg cgacaaacgc
     8401 gtgctggtgg tggatggcga gccggtaccg tactgcctgg cgcgtattcc gcaggggggc
     8461 gaaacccgtg gcaatctggc tgccggtggt cgcggtgaac ctcgtccgct gacggaaagt
     8521 gactggaaaa tcgcccgtca gatcgggccg acgctgaaag aaaaagggct gatttttgtt
     8581 ggtctggata tcatcggcga ccgtctgact gaaattaacg tcaccagccc aacctgtatt
     8641 cgtgagattg aagcagagtt tccggtgtcg atcaccggaa tgttaatgga tgccatcgaa
     8701 gcacgtttac agcagcagta acccacctta gcgagaagga tctcgttgag actctgagtg
     8761 acagcgccct tctttccacg catactgggc gctgttgctt ttttgaacca ggaaacagaa
     8821 cctctgacaa tgaatttaca gcatcacttt cttattgcca tgcctgctct ccaggatccg
     8881 attttccgtc gttccgtggt ctacatttgc gaacataata ccaatggtgc aatggggatc
     8941 atcgtcaaca agccgctgga aaatctcaaa attgaaggga ttctggaaaa gctgaagatc
     9001 acgccggagc cgcgtgatga atcaatccgt ctggataaac cggttatgct cggcggccca
     9061 ctggctgaag atcgcgggtt tattttgcat actccgccct ccaattttgc ttccagcatt
     9121 cgcatttcag acaacacggt gatgaccact tcccgcgatg tgctggaaac gctcggcacc
     9181 gataaacaac cgtctgacgt attggtggct ctgggctatg cctcctggga aaaaggtcaa
     9241 ctggaacaag aaattctcga taacgcgtgg ctaacggccc cggcggatct gaatattctg
     9301 ttcaaaacgc cgattgccga ccgctggcgc gaggcggcaa aactgattgg tgtggatatt
     9361 ctcaccatgc ctggtgtggc aggacacgcc tgatgagtgg aaccttactc gccttcgact
     9421 tcggcaccaa aagcattggc gtagcggtcg gccaacgcat taccggcacc gctcgccctt
     9481 tgcctgcaat taaagcgcag gacggtacgc cggactggaa cctcatcgaa cgtttactga
     9541 aagagtggca gcctgacgaa atcatcgtcg gtttgccgct gaatatggac ggcaccgaac
     9601 agccgttaac cgccagagcg cgcaaatttg ccaaccgtat tcatggtcgt ttcggtgttg
     9661 aagtaaagct ccatgacgag cgtcttagca ctgtggaagc ccgttccggt ctttttgaac
     9721 agggcggcta tcgagcgctc aacaaaggca aagttgactc tgcctctgcg gttattattc
     9781 tcgaaagcta tttcgagcag ggatattaag gcgatctaaa agcctggcgg atagtcgtta
     9841 ccgccatctt gccggatgcg gcgtgaacgc cttatccagc acacatctgg cagcggctgt
     9901 aggtctgata agacgcgtta gcgtcgcatc agacatttat tgcctttata ggccctataa
     9961 acttgcgcat cgggcatggc aacgtcacaa acgcccttcc cccacccgct gctgataact
    10021 ctgctgaaac gttaacatcc ccacctgctg cccggtttga ataacatgcg gtaactggtg
    10081 ggttttccct tcgcgaatca aattccccac cgcgggagtg ttaatcagta attcaaacag
    10141 cgccacgcgt ccttcctgtt tatccacttc cagcttttgt gacagcactg cccgtaaact
    10201 acccgcaagt tgattacgta cgggatcttt ttcctgcgcc ggaaatgaat ccaccagtcg
    10261 ctcaactgcc tgcgcggcac cacgtgtatg taaggttgcc agcaccagat gcccggtttc
    10321 tgccgccgtc agcgccagac ggattgtctc actgtcacgt agctctccga gcaaaatcac
    10381 atcaggatct tcccgcaatg cagcccgcaa ccccgatgcg aacgtcatac agtgcaaacc
    10441 aatttcccgc tgctggatca aacatcgctg gctggcatag agatattcca caggatcttc
    10501 cagcgtcaga atatgcgcat cggcatgttg attaagatag ccaaccatcg ccgccagcgt
    10561 ggtagatttg ccactccccg tagcccccgt caccagaatc aggccattct cgctcttgag
    10621 taattccggc aataccggtg gcgcaccaag ctgttcgagc tgtggacagt gcgaaggtaa
    10681 caaccgtaat gccagagaaa taccttgccg ttgcgcgaac gcactgccac gcaaccgctg
    10741 gttttccgcc agcgacacgg caaaatccag ctggccattt tccagcaata ttgcccgctg
    10801 atcgtcatcc agccactccc gcagtagctc ttcgacgtcc ggcgcatcaa acggcgcagc
    10861 ttccattctg ccacgaatgc gccatcgtgc gggccaggcg ctgcacaggt gtagatccga
    10921 gacgttatgc tttacactaa gggccacaat ttcttccata ttcatactaa gatcctcgga
    10981 aaatgaacga tattgcgcat aacctggcac aggtccggga caaaatctca gcggctgcaa
    11041 cgcgttgcgg ccgttctcca gaagaaatta cgctgcttgc agtcagtaaa acaaaacctg
    11101 cgagcgccat cgcagaagcc attgatgccg ggcagcgtca atttggtgaa aactacgttc
    11161 aggaaggggt agataaaatt cgccactttc aggaactggg cgtaacagga ttagaatggc
    11221 attttattgg cccgttgcag tctaataaaa gccgcctagt ggcagagcat ttcgactggt
    11281 gtcataccat cgaccgtttg cgcattgcta cccgcctcaa cgatcagcgc ccggcagaac
    11341 ttccccctct taacgtactg attcaaatta atattagtga tgaaaacagt aagtccggga
    11401 ttcaactggc agaactggac gagctggcag cggcggtcgc tgaactaccg cgtttacgtc
    11461 tgcgcgggct gatggcaatc cctgcgcctg agtcagaata tgtaaggcag tttgaagttg
    11521 cacgccaaat ggctgtagca tttgccggac tgaaaacgcg ctacccgcat atcgacacgc
    11581 tctctctggg aatgtcggac gatatggaag ccgccattgc ggcaggtagc acgatggttc
    11641 gtatcggcac tgcaattttt ggtgcgcgtg attactctaa aaaataagga attaaaggaa
    11701 cgccatgaat acgttgactt tcctgctttc aacggtcatt gagctgtata ccatggtgct
    11761 gttattacgc atctggatgc agtgggctca ttgtgatttt tacaacccct tctcacagtt
    11821 tgtagtgaag gtagcgcagc caattatcgg gccactgcgc cgcgttattc cggcaatggg
    11881 tcccattgac agcgcctcgc tgctggttgc ctatattctc agttttatca aagccattgt
    11941 gctgtttgaa gtggtgacct tcctgccaat catctggatt gccggtttac tgattctgct
    12001 gaaaaccatc ggcttgctga ttttctgggt cttgctggtg atggcgatta tgagctgggt
    12061 aagccagggt cgtagcccga ttgaatacgt gctgattcag ctggccgatc cgctgctgcg
    12121 cccgattcgc cgcttgctac cggcaatggg tgggattgat ttctcgccga tgatcctcgt
    12181 tctgctgctg tatgtcatca atatgggtgt cgcagaagta ttacagtcaa ccggaaacat
    12241 gctgctgccg gggctgtgga tggcgttatg agtgccgtaa cagttaatga tgacggtctg
    12301 gttttacggc tctatattca gccgaaagcc agccgtgatt ctattgtcgg tttacatggc
    12361 gacgaagtta aagtcgccat taccgcgccg ccggttgacg gccaggccaa cagtcatctg
    12421 gtgaagtttc tcggtaagca attccgggtt gccaaaagcc aggtggtgat tgaaaaaggc
    12481 gaacttggcc gccacaaaca aattaaaatc attaatccgc aacaaatccc gccagaaatc
    12541 gcggcgttaa ttaattaggt atcctatgca aaaagttgtc ctcgcaaccg gcaatgccgg
    12601 taaagtgcgt gagctggcgt cgctgcttag cgactttggt cttgatatcg tggcccaaac
    12661 agacctcggc gttgattccg ctgaagaaac cggcctaacc tttatcgaaa acgcgattct
    12721 gaaagcgcgc catgcggcaa aagtgaccgg tttaccggca attgccgacg actctggtct
    12781 ggcggtagat gcgctgggtg gcgcgccggg tatttactcc gcgcgttatt ccggtgaaga
    12841 cgcgaccgat caaaagaatc tgcaaaaact gttggaaaca ctgaaagacg taccggacga
    12901 ccaacgtcag gcgcgtttcc actgcgtgct ggtatatctg cgtcacgcgg aagatccgac
    12961 tccgctggta tgccacggca gctggccggg cgtgattact cgcgaaccag cgggcactgg
    13021 tggctttggt tatgatccaa tcttcttcgt accttccgaa gggaaaaccg ccgccgaact
    13081 gacccgcgaa gaaaagagcg ccatttccca ccgtggtcag gcgttgaaac tgctgctgga
    13141 cgctttacgt aatggttaaa ttaccgccgc tgagtctcta cattcacatc ccgtggtgcg
    13201 tgcagaaatg cccgtactgc gatttcaact ctcacgcgtt gaaaggagaa gtgccgcacg
    13261 acgattatgt tcagcatctg ctttgcgatc tggacaatga tgtggcttac gctcagggcc
    13321 gtgaagtaaa gacaatcttt attggcggtg gtacgccgag cctgctttcc ggcccggcga
    13381 tgcaaacgct gctggacggc gtgcgtgcgc gtttgccgct ggcagcggat gcagaaatta
    13441 ctatggaagc gaacccaggc acggtagaag cggatcgctt tgtcgattat cagcgtgctg
    13501 gtgtaaaccg catctctatt ggtgtgcaga gttttagcga agaaaagctg aaacgacttg
    13561 ggcgtattca tggcccgcaa gaagcgaaac gagcggcaaa gctggcgagc ggtttagggt
    13621 tacgtagctt taaccttgat ttgatgcatg gactaccgga tcaatcactg gaagaggcgc
    13681 ttggcgatct acgccaggcc attgaactga atccgccgca tctttcctgg tatcaactga
    13741 ccatcgaacc taatacgctg tttggctcgc gaccgccggt gctgccggac gatgacgcgc
    13801 tgtgggatat tttcgaacag gggcatcagt tattaaccgc agcgggttat cagcaatatg
    13861 aaacttccgc ttacgccaaa cccggttatc agtgccagca caatctcaac tactggcgct
    13921 ttggtgacta catcggtatt ggctgcggcg cgcacggcaa agtgaccttc ccggatgggc
    13981 gcattctgcg taccactaaa acgcgtcatc ctcgtggttt tatgcaagga aggtatctgg
    14041 aaagccagcg tgatgtcgaa gccgcagata agccgtttga gttctttatg aatcgcttcc
    14101 gtctgctgga ggccgcgccg cgcgtggagt ttattgcgta taccgggctt tgcgaagatg
    14161 tgattcgccc acagttagac gaggcgattg cccagggtta tctcaccgaa tgtgcggatt
    14221 actggcagat aacggaacat ggtaaattat tcttaaactc acttcttgaa ttattcctcg
    14281 ctgagtaatc attaatgcgc ctgcgggcgc attaatcact tcttttatcc ccccgttaat
    14341 taacctcggc agaaatatat ttcttactga ataaatatcg acgacattta ttatatatat
    14401 ttagtgaata tttactaatc attcattgat tataaatatg tacaacctgg cgcacaaatt
    14461 taatatttaa tttctgactt tatttcaagt tgtgataaac atagacaagt tgtcagacag
    14521 gttaaccaat ttgtcgcaca aagcactgat aagcacaatt agatatcaac caatttccat
    14581 ccttcaacag ggagtttttt tatgaaaatg aggtcattta cccgctcatt agtctgcgca
    14641 tccttgctgg ctctggttag cactggcgta aacgccgcag aaaaagtgac gttaaagtta
    14701 gcacacaacc tggaaagaag tcatgttgta catcagagtt ttgaagagct ggcgaaagag
    14761 gtaaaacagc tgtcaaaagg taatatggtg atccgtattt atcccagcag ccagatgggt
    14821 aatgcgcgag aaactatgga attgttgcaa aatggcgcac tggatatgac caaaggttcc
    14881 gccagtgatc tggagtcttt tgataatatt tatgctatct ataatttgcc gttcttatta
    14941 agttgttttt ggtgaagttg gtaaagaaat tatggactca acaaaagaca aaggcttttt
    15001 tgctttatca gcttatgtag cgggtactcg cagtttctat gcgaaaaaac cgattacaaa
    15061 gccagaagat cttaaaggac tgaaaattcg tgttcagcca agtccgacca ccatcaaaat
    15121 gattgaactg atgggtggtt cacccactcc aatttcattt ggcgaagttt atactgcgat
    15181 gcagcaaggt gtagtagatg gcgcagaaaa taacgtgcct tcctgggtgc agactcgaca
    15241 tattgaaatt gccaaagttt tttctgaaga tgaacacgcc tcaattcctg actttttggt
    15301 tatctccacc aaaacatgga ataaattaac cccagaacag caacaaatcc tcgaaacagc
    15361 ggcgaagaaa tccgaagctt atcaacaaaa attgtgggaa aaaattgacg ctgacacacg
    15421 tgctcaggcg aaagctatgg ggggtgaaat tgtaaaagtg gataaagcgc cattccgtaa
    15481 agccgtgcaa cctctgtttg acgacttcaa aaaagatcca aaacaagctg ctttgttaga
    15541 gaaattcgac aacgctgctc aataatattc cgggccagcc tctggccctg attttccagg
    15601 atgcttacaa tgatatttaa ccgcctgaaa cttgcggtag accgcgtcat tgccgcgttt
    15661 tctgtcgccg taatgcttgc tctggttgta tgcgtagtct ggcaagtatt tagccgctac
    15721 gtacttaacc agccaagtac attaaccgat gagctggcac gctttttgat gatctgggta
    15781 ggattattag gtgctgcata taccgtcggc gcgcagcgac atctctctat tgatttattt
    15841 gctctcgccc tgaataagcg caaacaatta cttcttagta tagtcattaa cgttctcatt
    15901 ttaggttttg ccggttcggt cattgtgact ggtggcctga aattaattga taaaacactg
    15961 gcaacatcac aggtatctgc cgctatgcaa ataccgatgg gatacgtata tatcattctg
    16021 ccattaagtg gagtggtaat gatgttttat gccttgtgtt ttattaatca aagcattcaa
    16081 caactgaaac aacctgcgca ggaggccagt taatggatag ttatatcgcg attacactgt
    16141 ttggctgttt ttttgtgctg gtctttattg gtgtgcccat ttcgttctca atcggtattg
    16201 ccactgtagc ttcaatgctt ttgatgtttc cctgggatat cgcggcaatc actgtttcgc
    16261 aacgactggc taatggactg gataactttg ccctgttggc aatccccttt ttcatctttg
    16321 ccggaacact gatgaacagc ggcggtattg cgatacgttt gattaacctc gcccaggtaa
    16381 tggttggccg cgttcctggt tcgcttggtc atgtgaacgt cctggcgaat atgatgtttg
    16441 gttccatttc tggttcagcc gttgcggcag ctgctgccgt tggtggcaca ctgaatccca
    16501 ttcagaccaa agaaggttat gatccggcat tttctaccgc tgtaaacgtc tcatcttgta
    16561 ttactggatt actgataccg ccctcaaacg tgttaattgt gttttccctg acagctggcg
    16621 gcgtttctgt agcatcgctg tttatggcgg gatatttgcc agggatctta atgggtcttg
    16681 cggtaatgat agtctgcggc attattgcta aacgccgtgg ctacccgctt tcagaaagag
    16741 caacctttgc tcaggcctgt aaagcgtttc tggatgcctt accaagtttg cttctggtgt
    16801 tcattgtgat gggcggcatt ctgggcggca tttttaccgc aactgaagcg tcagcaattg
    16861 ccgttgtcta cacatttatt ctctcggtgc tgatttaccg ggaagtaaaa tggcgccatt
    16921 tacccaaact gattctggag tcggttgtta ccacttcgat tgtattactg ttgatcggtt
    16981 tttccgtggg catgtcctgg gcaatgacta atgctgatat tccttatatg atcagcgacg
    17041 cattgatggg gatttctgat aacccactga tcatattgct gctgattaat atcgtcttat
    17101 taattgtcgg catctttatg gacatgacac cggctgtact aatctttaca ccgatcttct
    17161 tgccgattgc acaagagctg ggaatggacc cagtacattt cggtattatg atggtggcca
    17221 acctttgtat tgggctgtta acaccgccgg tgggtagtgc gctatttgtt ggttgttcta
    17281 tctcgggtgt gaagattcaa catttaatca aaccattgct gccattttat gctgcattgc
    17341 tagtagcgtt gatgatgatc acttacattc cgcaaatatc cttgttcatt ccacagctac
    17401 tagggctaat gtaaaaaata atttgccgga taacttttta tattatccgg caaaggcact
    17461 taacatgagt tacttaatca ccccattgcg ccgcagttgt tcggtatctt ttttccagcg
    17521 ttcattagct tggttgatat cgtcaatttt catcaaaatc gcgacttttt tactgtttat
    17581 ttctgaaact ttcatcgtta acggatattg ccctttttcg agcgttaccc agtcatcctg
    17641 caatttacgg ctgagttttt gtgattgcag aaaagtctgt cgccgttgtt cgttgatatc
    17701 gaaatctcgt ttctgctgaa tcggcacttc ataacactct tcactctgtt tatgacatgc
    17761 cgcaatcgcc ggttgataaa cctctttgtc atatttaacc gcgaacgcct cgctatcgtt
    17821 ctgtttatta aactcatcac gctgttggtg aattttttta aaggcttctt cgcgggtcat
    17881 ttgcttaccg tttgcatctt ttccccaata tgcatccgct gctttctgcg ccgccaccat
    17941 ttgcttatca agcgctttca cttccttttc cagcgcagga atcgagttct tctgggtatt
    18001 caacgcagat tcaagctgac taaagcgata attgaaatct tcgctattca cgatcagata
    18061 tttattattc gttttgatat cgtcgaccac ccgcccacga tcgctggctg ccgcctgaaa
    18121 agagtaaaag ttcaccgacc agccagacgc gggcgttcct ttactggtta acatcgccga
    18181 gaattttacc ggtttatctt tcgtccaggt ctgttcgagc agttcgtaat ctgccaactg
    18241 accgacccag gtatagagat cgtcactgga agagacatcg ccttccgctg accaggtggc
    18301 ctgattaccc ttagcatcaa gattttttaa gctgatggca tcaagcttga gaatgccgtg
    18361 atactgcttt ttgaattgat cttttaagat attttcagtt ggcggttcgc catctgccca
    18421 ggcattacca gtcatcaaca taagcagcgc cgtgccaaca atccattgct ttttcacccg
    18481 aaaaccctcg cgcggaataa tttattccgg tgattatcat tagggtaatg aaaattggca
    18541 atggacgtaa gtctgaagtg aaaaagcccc ggcacgatgc cgggacgagg tgattagtac
    18601 tgattgaaaa tctgctggat ctgctgcgga tctttggttt gcgtcagagc cagctgcagc
    18661 aggacgcgcg ctttttgcgg gttcagcgtg ccagaggcga cgaagccgta tttcgcatca
    18721 tccacttcag catcctgagt ggtagcaccc gtcggtacgc gggaagaacg cactactgca
    18781 gtgccgtttt tcgcggcggt tgccagggtg tcgaacacgg atttatacag gttaccatta
    18841 cccacaccag cgctaacgat gccatcatag cccgcatcta ccagtgcttt agccggaaga
    18901 tcggatgcgt tagcgtagtt ataaacgatg ccgactttcg gcagctcatt cagcttagag
    18961 acatcgaatg gcgtatcgct ggtgtgctta cgtgccgggg tacgttggta gtcaatctta
    19021 ccgttgtgaa tgtatcccag aggaccgtag ttaacagact tgaaggtcgc tacgtcggtg
    19081 gtgttggttt tggtgacatc gcgaccgtcc agtacggtgt cgttcatcac caccagcacg
    19141 ccacgattag cggaggcttt atcagctgcg gtcactaccg cgttatacag gttgaatgga
    19201 ccgtctgcgc tcatggacgt ggacgggcgc attgcgccga ccatcaccac cggtttgtcg
    19261 catttcaccg tcaggtcgag gaagtaagca gtttcttcca tcgtgtcggt accgtgggta
    19321 atgacgaagc cgtcggtttt atcgcagtcg gtgttaattt ttttcgccag tgtcagccag
    19381 acattatcgt tcatgtcctg ggagccgata ttcactacct gctcgccttt aacgttcgca
    19441 atgtctttta gttgcggcac cgcattaacc agattttcta cgccaacttt acccgctgtg
    19501 tagttagatt tggttgcgga gtcaccacca ccggcaatgg tcccgccggt tgctaaaatg
    19561 gtgatattgg gtaatgccaa tgctgcacca ctaaaaccca taaccagtgc ggcaagagcc
    19621 gtctttttga aaaactccat ttcattcctc cagttacgtg aacgctacgc attatccctt
    19681 agctttgtat gggaaatttg acgttaaaca atttacaacg tgaatatatt ttggagatct
    19741 acaaagttag aggcaggtaa caaaacgaag aattaaacgg cataaaaaag tattatgccg
    19801 tcttaaaata gaggattatt ttaaattccc gaccagggct ttgcggctat cttccagagt
    19861 cacaacgcgg ctacaaacat ctttgccaaa ctgctggaaa tctttttcct gctttttcca
    19921 ctcggtttgg attgaggatt gcagaccgcc caagcttccc agcacattct gtaatgggtt
    19981 accgccgctt ttcagcaccg ctttcgcgcc catttcatta atgctgtcct gtaaaattcc
    20041 gcccattgcc tgattcacta gttgctggcc ttcggcgcga acctgatcaa tggctttata
    20101 gtggaacgtc aggccatcgc tgcgcgtttc gataatgcga ttcatctgct ctttaagctg
    20161 cgcatcaagt ttggtcagac ggctgcgcat tttgctgctg tcgcccatct cctgaacgat
    20221 aattttatcc agcgcaatac gggctttctc gacgcggctt ttcgcgcctt catcaatcca
    20281 cggcagcgtg ctacgtagtt cagcctgata atccttcgcc tgctcgcgct gggcggcatt
    20341 cagggaatat tgcttaccgt tatacatcac gttgccgtct ggcgtgatca ccagattgcc
    20401 gttttcgccc ttcacctgca cggtttgcgg gctgacaatc acatcgtcac gcggcgtgac
    20461 gctgcactgg tagtcggcgt gagcggtcat tgccgtcact gaaagtgctg ccgccagcag
    20521 cattttgcgc atcatagtct tccctcaaga acaaatcagg ccagcatatg ctggccccag
    20581 attgataaca aagtacgcgt tttccatgcc ggatgcggcg taaacgcctt atcccgccta
    20641 ctcgatcgtg catctctgta ggcctgataa gcgtagcgca tcaggcagtt ttgcatttgt
    20701 catcgcccgt atgctttctt agtcccacca aacgtcgaaa agttcgctgg tgcgtacctc
    20761 atccagtttg cactcttcca gccacttacg cacaatcgcc tgatgttctt cggtgcattt
    20821 gccgatttcc tgcatgcaga tccgaccttc ccaggccaga taaccgctgc cgtcaaaggc
    20881 cagtttgttc ggttcgataa cctcgttaat aaagtcatca acgattttat caatctgttc
    20941 ttccgatgta ccttccggga atcgccatgc caccgaaaat cctaattcct ggaattcgtc
    21001 gatgtgcatt tttttacgca gacgacggct acggttcttt gccattattt caccctctcg
    21061 aacattaagt cccatactcc gtgaccaaga cgatgaccac gttgttcaaa tttcgtcacc
    21121 ggacgtgatg ccggacgcgg tacgtaatca ttgctctctg acaggttttt ataaccgtca
    21181 atagaagaca tcacttcaag catatgttcc gcataaggtt cccagtcggt cgccatatgg
    21241 aatacgcccc ccagctgcag tttgcttttt accagttcgg caaacggcac ctgaacgata
    21301 cggcgtttat tatggcgcgc tttgtgccac gggtcaggga aaaagagctg caccatgcgc
    21361 aatgaattgt caggaatcat tttatgcagc acttcaaccg catcgtgaca catcacgcgc
    21421 aggttgctta aaccttcttc atgcgcagaa gccaggcacg caccaacgcc cggtgaatgc
    21481 acttcaatgc cgaggaagtc ctgctcgggg cgatctttag ccattgccac cagcgacgcc
    21541 cccatgccaa aaccaatctc aagcgtcacc ggcgcttcac ggccaaaaag cgcggggaaa
    21601 tccagcatat cttcactgaa ctcaacgccc atcaccggcc agtagttttc cagcgcatgt
    21661 tcctggcctt tggtcagtcg cccctggcgg cgcacaaaac tacggatacg gcgcagtggg
    21721 cggccgtttt catcaaattc cggtgaaatg acgtcgtttt tcataaagat ttagtcgctt
    21781 gtgaaagtgt tctgaaaacg ggcattatcc aaagttagtt gccggatgca agcatgataa
    21841 ggccgtggct gcggaaagtt ccggtttaca ccctgccgtc gctgtgctgc aatcttgccc
    21901 ccaacaacag tgaagtcggt gaccatgcaa gcgtcgcaat tttcagccca ggttctggac
    21961 tggtacgata aatacgggcg gaaaacgctg ccctggcaaa ttgacaagac gccctacaaa
    22021 gtatggctct cagaagtgat gttgcaacaa actcaggttg cgaccgttat cccctatttt
    22081 gaacgcttta tggcgcactt cccgacggtg accgatctcg ccaatgcgcc gctcgacgaa
    22141 gttctccact tgtggaccgg gcttggctat tacgcccgcg cgcgcaacct gcataaagcg
    22201 gcacaacaag tggcgacctt acacggcggt aaattcccgg aaacctttga agaagtcgcg
    22261 gcgttaccgg gcgtcgggcg ttccaccgca ggcgcgattc tctcgctttc tctgggtaag
    22321 cactttccga ttctcgacgg taacgtcaaa cgcgtgctgg cgcgctgcta tgctgtaagc
    22381 ggctggcctg ggaaaaaaga ggtcgagaat aaactatgga gtttgagcga gcaggtgacg
    22441 cccgcggtcg gcgtggaacg gtttaatcag gcgatgatgg atttgggcgc gatgatttgc
    22501 acgcgttcga agccgaaatg ttcgctctgt ccgctacaaa acggatgtat tgccgccacc
    22561 aacaatagct ggtcgcttta tccgggcaaa aaaccgaaac agacgctgcc ggagcgcacc
    22621 ggctactttc tcctgttaca gcacgaagat gaagtattgc tggcgcagcg tccgccgagc
    22681 ggattgtggg gcggtttata ctgtttcccg cagtttgccg acgaagaaag tttgcggcat
    22741 tggctggcac aacggcagat tgctgccgat aacctgacgc aactgaccgc gtttcgccat
    22801 accttcagcc atttccactt agatattgtg cctatgtggc ttcccgtgtc gtcattcacc
    22861 agctgcatgg atgaaggcaa tgcgctctgg tataacttag cgcaaccgcc gtcagttggc
    22921 ctggcggctc ccgtggagcg tttgttacag cagttacgca ctggcgcgcc ggtttagcgc
    22981 gtgagtcgat aaagaggatg atttatgagc agaacgattt tttgtacttt cctgcaacgt
    23041 gaagcagaag gtcaggattt tcagctgtac cccggcgagc tgggaaaacg catctataac
    23101 gagatctcca aagaagcctg ggcgcagtgg cagcacaaac aaaccatgct gattaatgaa
    23161 aagaaactca acatgatgaa tgccgagcac cgcaagctgc tggagcagga gatggtcaac
    23221 ttcctgttcg agggtaaaga ggtgcatatc gagggctata cgccggaaga taaaaaataa
    23281 aaacagtgcc ggagcacgcc tccggcagct tgcataaaaa caaacacaac acgcacccgg
    23341 aatgatgaaa aaatatctcg cgctggcttt gattgcgccg ttgctcatct cctgttcgac
    23401 gaccaaaaaa ggcgatacct ataacgaagc ctgggtcaaa gataccaacg gttttgatat
    23461 tctgatgggg caatttgccc acaatattga gaacatctgg ggcttcaaag aggtggtgat
    23521 cgccggtcct aaggactacg tgaaatacac cgatcaatat cagacccgca gccacatcaa
    23581 cttcgatgac ggtacgatta ctatcgaaac catcgccggg acagaacctg ccgcgcattt
    23641 gcgccgggca attatcaaaa cgttgctgat gggtgacgat ccgagttcgg tcgatctcta
    23701 ttccgacgtt gatgatatta caatttcgaa agaacctttc ctttacggtc aggtggtgga
    23761 caacaccggg cagccgattc gctgggaagg tcgcgcaagc aacttcgcgg attatctgct
    23821 gaaaaaccgt ctgaaaagcc gcagcaacgg gctacgtatc atctacagcg tcaccattaa
    23881 catggtgccg aaccaccttg ataaacgtgc gcacaaatat ctcggcatgg tccgccaggc
    23941 gtcacggaaa tatggcgttg atgagtcgct gattctggca attatgcaga ctgaatcttc
    24001 ctttaacccg tatgcggtca gccgttccga tgcgctggga ttaatgcagg tggtacaaaa
    24061 tactgccggg aaagatgtgt tccgctcgca ggggaaatcc ggcacgccga gccgcagttt
    24121 cttgtttgat cctgccagca atattgatac cggcaccgcg tatctggcga tgctgaacaa
    24181 tgtttatctc ggcggaattg ataacccaac atcgcggcgt tatgccgtca tcaccgccta
    24241 taacggcggc gcaggcagcg tgctgcgagt cttttcgaat gataagattc aggctgccaa
    24301 tattattaac accatgacgc cgggcgatgt ttatcagacg ctgacgaccc gccatccctc
    24361 tgcggaatct cgccgttatc tttataaagt gaataccgcg caaaaatcct accgccgccg
    24421 ataattccat taaccgcccc tgacgatgct caggggcaaa aatgttatcc acatcacaat
    24481 ttcgttttgc aaattgggaa tgtttgcaat tatttgccac aggtaacaaa aaaccagtcc
    24541 gcgaagttga tagaatccca tcatctcgca cggtcaaatg tgctttttca aacactcatc
    24601 cgcatcacga tgtgaggaaa ttaacatgaa tcttaagctg cagctgaaaa tcctctcttt
    24661 tctgcagttc tgtctgtggg gaagttggct gacgaccctc ggctcctata tgtttgttac
    24721 cctgaagttt gacggtgctt ctattggcgc agtttatagc tcactgggta tcgccgcggt
    24781 atttatgcct gcgctgctgg ggattgtggc cgacaaatgg ttaagtgcga aatgggtata
    24841 tgccatttgc cacaccattg gcgctatcac gctgttcatg gcggcacagg tcacgacgcc
    24901 ggaagcgatg ttccttgtga tattgattaa ctcgtttgct tatatgccaa cgcttgggtt
    24961 aatcaacacc atctcttact atcgcctgca aaatgccggg atggatatcg ttactgactt
    25021 cccgccaatc cgtatctggg gcaccatcgg ctttatcatg gcaatgtggg tggtgagcct
    25081 gtctggcttc gaattaagcc acatgcagct gtatattggc gcagcacttt ccgccattct
    25141 ggttctgttt accctgactc tgccgcatat tccggttgct aaacagcaag cgaatcagag
    25201 ctggacaacc ctgctgggcc tcgatgcatt cgcgctgttt aaaaacaagc gtatggcaat
    25261 cttcttcatc ttctcaatgc tgctgggcgc ggaactgcag attaccaaca tgttcggtaa
    25321 taccttcctg cacagcttcg acaaagatcc gatgtttgcc agcagcttca tcgtgcagca
    25381 tgcgtcaatc atcatgtcga tttcgcagat ctctgaaacc ctgttcattc tgaccatccc
    25441 gttcttctta agccgctacg gtattaagaa cgtaatgatg atcagtattg tggcgtggat
    25501 cctgcgtttt gcgctgtttg cttacggcga cccgactccg ttcggtactg tactgctggt
    25561 actgtcgatg atcgtttacg gttgcgcatt cgacttcttc aacatctctg gttcggtgtt
    25621 tgtcgaaaaa gaagttagcc cggcaattcg cgccagtgca caagggatgt tcctgatgat
    25681 gactaacggc ttcggctgta tcctcggcgg catcgtgagc ggtaaagttg ttgagatgta
    25741 cacccaaaac ggcattaccg actggcagac cgtatggttg attttcgctg gttattccgt
    25801 ggttctggcc ttcgcgttca tggcgatgtt caaatataaa cacgttcgtg tcccgacagg
    25861 cacacagacg gttagccact aattacgcaa agaaaaacgg gtcgccagaa ggtgacccgt
    25921 ttttttattc ttacttcaac acataaccgt acaatcgttt catgccatcc gcatcggttt
    25981 cgctatagac accttgcaac tccggcgaaa atcccgacaa caaattcacg ccttcttcca
    26041 gcgcaaggaa ataacgttga accgccccac cccagacttc cccgggtacc acgcaaagca
    26101 cgccaggtgg ataaggcaac gccccttctg ccgcaattcg cccttcggca tcacgaatcc
    26161 gcaccaactc cacttcaccg cgaatataag cgctatgcgc atcctggggg ttcatcacca
    26221 ctgacgggaa actctgctgg cggaacatcg ctttttgtag gtctttgacg tcgaaactga
    26281 catacagatc gtgcatctcc tgacacaact ggcgcagggt gtagtcgcga tagcgcaccg
    26341 gatatttgtt ataaacgctc ggcaacacct cagccagcgg cgagtcatcc tcaatatgct
    26401 gttcaaattg cgccagcatc gccaccagtt gtgccagctt ctcgtggctt tccgccggag
    26461 ttaataagaa cagaatggag ttgagatcgc acttctccgg cacaatgccg ttctcacgca
    26521 gatagtgcgc cagaatcgtc gccggaacgc caaagtcgct atattcgccg gtttcggcat
    26581 tgatacctgg tgtagtgagt aacagcttgc acggatcaac aaaatactga tccgcggcat
    26641 atccttcaaa gccgtgccac ttcgcccccg gcacaaaact gaaaaaacgg cggtcgctgg
    26701 ctaacactga tgtcggataa tcctgccaca atttgccatc aacaacgggc gggataaacg
    26761 ggcggaacag cttacagcgc gcaagaatag ccttgcgcga ctcaataccc aactcaacgc
    26821 actcagccca cagccgacgc ccactctccc cttcatgaat tttggcgtta acatccagtg
    26881 cagcaaacag cggatagaaa gggctggtag aagcatggag cataaaggcg ttattcaacc
    26941 gcttatgcgg gcaaaaacgc gcctgtccgc ggatatggtt atctttttta tggatctgcg
    27001 acgtctgtga gaatccagcc tgctgtttgt gcaccaactg tgtcacaaaa atccccggat
    27061 cgttttcgtt aagttctaac agcagcggcg agctatccgc catcatcggg ataaactgtt
    27121 cgtaaccgac ccacgcggaa tcaaacagaa tgtaatcaca cagatgccca atggtatcga
    27181 tcacctgacg ggcgttatag acagtgccgt cataggttcc cagctgaata atcgccaggc
    27241 gaaacgggcg cggcaggtcg gctttttctg gcgcaacgtc gcgaatttgc tggcgcagat
    27301 actcttcatt aaaacagtgc gcatcaatac cgccaatgaa accaaacggg ttacgtgaag
    27361 cttccagata gaccggcgtc gtccccgcct gaatcagcgc gccgtgatga ttcgacttat
    27421 ggttgttacg gtcgaagagc accagatcgc cacgcgttaa cagcgcattc gtcaccactt
    27481 tattcgctgc cgatgtgccg ttcagcacaa aataggtttt atcggcatga aagactttgg
    27541 ctgcgaattt ctgcgcatct ttcgccgatc cttcatgaat aagcagatcg cccaatttta
    27601 cgtcagcgtt acacatatcg gcgcgaaaga cgttctcacc aaagaaatcg taaaaatggc
    27661 gtccggcagg atgcttttta aagaacgcac catgttgatg tccagagcaa gcaaaggtgc
    27721 tgttgcccat ctcaacgtac tgcgtcagcg tgtcataaaa cggtggcagc aaattctctt
    27781 catactgaca ggctgcggat tccagctcca gccactgctg ctcgttgccg ttgattaccg
    27841 ccgtaacgcc cgcaggtaat tcaacagcat gttcggaata caaaaacacc ggtagatgaa
    27901 aaccggtgcg cttaagcaac gcaagaatgc cactgcgact atccgcagcg gtaatgacga
    27961 ctgccgcgac gtccgtaaaa tcagtatctc ccaacgccac cacgcggcga tgagaagaaa
    28021 gtcgggatac cagttcacta ctggcggcaa tattcattga tttcataagc gcaaacccgt
    28081 ttcggggaag taagaatacc ggacaaggtg gaaaaccctg ccccatgaga tatgggtcaa
    28141 actggtcacc agctccgacc gccagacatc agtaaaagca gaaacgctct gattttactg
    28201 ttgtcctgca gtgagcgtgc gttaacttca ccgcatgagc agtaacatag aaagggaaac
    28261 gtttcgcgcg aaacggcgat aagcgagaga atgtaaggag atggcgtgca tcgggcaaac
    28321 tccgtacaga gaggagaaaa ttcgcgcaat catggcacct ttcgctaagg cgtgcaagcc
    28381 aggactttgc gaccagacaa gccatcaaca aggcaaaaag ttcataataa gaaaatcaaa
    28441 caatacagct aacaggaact cttgtggtca tcggcccttt tatcaacgct agtgcagtct
    28501 tactgggtgg cgttctcggc gcactgctca gccaacgctt accggaacgt atccgcgtct
    28561 ccatgacatc tatttttggt ctggcatcgc tggggattgg tattttactg gttgtgaaat
    28621 gcgccaacct tccagcgatg gttttagcta cgctacttgg cgctctaatt ggcgaaattt
    28681 gtttgctgga gaaaggtgtc aatacagcag tcgccaaagc acaaaatctg tttcgtcact
    28741 cacgtaagaa gccagcgcat gaatctttta ttcagaatta tgtcgcgatt attgtcctgt
    28801 tttgcgccag cggcaccggg atcttcgggg cgatgaacga agggatgacc ggcgatccga
    28861 gtattttaat cgccaagtca tttcttgatt tctttacggc gatgatcttc gcctgctcgc
    28921 tgggtattgc ggtgtcggta attagtatcc cattactgat catccagtta acgctggcgt
    28981 gggctgccgc gctgatatta ccgctgacca caccgtcgat gatggcagac ttcagcgccg
    29041 taggcggttt attgctgctg gcaaccggat tacgcatctg tggcattaaa atgttcccgg
    29101 tggtcaacat gcttccagca ctccttctgg caatgccgct ttccgccgcc tggaccgcct
    29161 agtttgcctg acaatgcgtg caatagcggc aaagtgatga tagattgtgc agtctgcagt
    29221 aaattgaaga aatttgattg acgagacgag gcgaatcagg tttaatgcgc cccgttgccc
    29281 ggatagctca gtcggtagag caggggattg aaaatccccg tgtccttggt tcgattccga
    29341 gtccgggcac cactatttag aagaaccagc ctacgggctg gttttttgct ttctggggtt
    29401 ctggtagata tccggtcact ttgaatgctg atgtcactgc tttacctctt ccatccagcc
    29461 tgaacatttc ttctaagtag cccccttctg gtcactgtgg ccttttctgt caaacttcaa
    29521 atccccaata gacactattt ccacatgagc acctggctaa cggattcatt actgcacgga
    29581 tacatccggt ctttgtccgg tctgaagtaa aacgcatacc acctgcgttg gcccctcctc
    29641 agtcccccct acatcaaacc gaacattttt cactctgatt gatttagctt tagtcaactt
    29701 ttgggt
//
LOCUS       DBKEKI010000041        29695 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.041, whole genome shotgun sequence.
ACCESSION   DBKEKI010000041 DBKEKI010000000
VERSION     DBKEKI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.041"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..940)
                     /locus_tag="ACUQPX_001787"
     CDS             complement(<1..940)
                     /locus_tag="ACUQPX_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HHV9056517.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVA"
     gene            complement(970..1308)
                     /gene="glnK"
                     /locus_tag="ACUQPX_001788"
     CDS             complement(970..1308)
                     /gene="glnK"
                     /locus_tag="ACUQPX_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HHV9056518.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(1489..3270)
                     /locus_tag="ACUQPX_001789"
     CDS             complement(1489..3270)
                     /locus_tag="ACUQPX_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HHV9056519.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(3263..5035)
                     /locus_tag="ACUQPX_001790"
     CDS             complement(3263..5035)
                     /locus_tag="ACUQPX_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HHV9056520.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(5065..5523)
                     /gene="decR"
                     /locus_tag="ACUQPX_001791"
     CDS             complement(5065..5523)
                     /gene="decR"
                     /locus_tag="ACUQPX_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HHV9056521.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(5677..6495)
                     /gene="cof"
                     /locus_tag="ACUQPX_001792"
     CDS             complement(5677..6495)
                     /gene="cof"
                     /locus_tag="ACUQPX_001792"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HHV9056522.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATYCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            6595..8295
                     /locus_tag="ACUQPX_001793"
     CDS             6595..8295
                     /locus_tag="ACUQPX_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HHV9056523.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPS
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            8360..9055
                     /gene="queC"
                     /locus_tag="ACUQPX_001794"
     CDS             8360..9055
                     /gene="queC"
                     /locus_tag="ACUQPX_001794"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HHV9056524.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(9107..9505)
                     /gene="fadM"
                     /locus_tag="ACUQPX_001795"
     CDS             complement(9107..9505)
                     /gene="fadM"
                     /locus_tag="ACUQPX_001795"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HHV9056525.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(9599..9970)
                     /locus_tag="ACUQPX_001796"
     CDS             complement(9599..9970)
                     /locus_tag="ACUQPX_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HHV9056526.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(10121..11992)
                     /gene="ppiD"
                     /locus_tag="ACUQPX_001797"
     CDS             complement(10121..11992)
                     /gene="ppiD"
                     /locus_tag="ACUQPX_001797"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HHV9056527.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(12184..12474)
                     /gene="hupB"
                     /locus_tag="ACUQPX_001798"
     CDS             complement(12184..12474)
                     /gene="hupB"
                     /locus_tag="ACUQPX_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HHV9056528.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(12665..15019)
                     /gene="lon"
                     /locus_tag="ACUQPX_001799"
     CDS             complement(12665..15019)
                     /gene="lon"
                     /locus_tag="ACUQPX_001799"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HHV9056529.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDNSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(15207..16481)
                     /gene="clpX"
                     /locus_tag="ACUQPX_001800"
     CDS             complement(15207..16481)
                     /gene="clpX"
                     /locus_tag="ACUQPX_001800"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HHV9056530.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(16607..17230)
                     /gene="clpP"
                     /locus_tag="ACUQPX_001801"
     CDS             complement(16607..17230)
                     /gene="clpP"
                     /locus_tag="ACUQPX_001801"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HHV9056531.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(17477..18775)
                     /gene="tig"
                     /locus_tag="ACUQPX_001802"
     CDS             complement(17477..18775)
                     /gene="tig"
                     /locus_tag="ACUQPX_001802"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HHV9056532.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(19119..19436)
                     /gene="bolA"
                     /locus_tag="ACUQPX_001803"
     CDS             complement(19119..19436)
                     /gene="bolA"
                     /locus_tag="ACUQPX_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HHV9056533.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            19741..20319
                     /locus_tag="ACUQPX_001804"
     CDS             19741..20319
                     /locus_tag="ACUQPX_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HHV9056534.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            20363..21838
                     /gene="ampG"
                     /locus_tag="ACUQPX_001805"
     CDS             20363..21838
                     /gene="ampG"
                     /locus_tag="ACUQPX_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HHV9056535.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            22298..23245
                     /gene="cyoA"
                     /locus_tag="ACUQPX_001806"
                     /pseudo
     CDS             22298..23245
                     /gene="cyoA"
                     /locus_tag="ACUQPX_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
     gene            23267..25258
                     /gene="cyoB"
                     /locus_tag="ACUQPX_001807"
     CDS             23267..25258
                     /gene="cyoB"
                     /locus_tag="ACUQPX_001807"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HHV9056536.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNCDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            25248..25862
                     /locus_tag="ACUQPX_001808"
     CDS             25248..25862
                     /locus_tag="ACUQPX_001808"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HHV9056537.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMKIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            25862..26191
                     /locus_tag="ACUQPX_001809"
     CDS             25862..26191
                     /locus_tag="ACUQPX_001809"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HHV9056538.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            26203..27093
                     /gene="cyoE"
                     /locus_tag="ACUQPX_001810"
     CDS             26203..27093
                     /gene="cyoE"
                     /locus_tag="ACUQPX_001810"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HHV9056539.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            27278..27589
                     /locus_tag="ACUQPX_001811"
     CDS             27278..27589
                     /locus_tag="ACUQPX_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HHV9056540.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSVAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            27573..28114
                     /locus_tag="ACUQPX_001812"
                     /pseudo
     CDS             27573..28114
                     /locus_tag="ACUQPX_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(28170..29105)
                     /locus_tag="ACUQPX_001813"
     CDS             complement(28170..29105)
                     /locus_tag="ACUQPX_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HHV9056541.1"
                     /translation="MLPNRILFSLMLIVSSGVVASKKAHELYDSIYGGKSAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGSIYYD
                     GEQVRVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKKINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(29116..29349)
                     /locus_tag="ACUQPX_001814"
     CDS             complement(29116..29349)
                     /locus_tag="ACUQPX_001814"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056542.1"
                     /translation="MSKEYTLSVPPFSLNTLGVDMYNNSSHIRKSYIDIPMYNRNLCD
                     FHDLTYSIKSATAILNGKQRDLLKEEHIQVQDK"
     gene            complement(29342..>29695)
                     /locus_tag="ACUQPX_001815"
     CDS             complement(29342..>29695)
                     /locus_tag="ACUQPX_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HHV9056543.1"
                     /translation="QGAKKAALWLSWMYYNGLGETLDCDKVWDYYEKGAGKYSMKFNK
                     DEYLTRCNQDQKTRETQGDTLPYITLKHYGTYGFNANEKPKICNVSIEINADKISSIT
                     NLQLTLELKGGGGSE"
BASE COUNT         6956 a         7962 c         7137 g         7640 t
ORIGIN      
        1 cagctaccac gccgataatc aacgcgccgc caaccccaat gtagccgcac gctggcgtca
       61 cgccgaccag accggcaatc gcgccagaac acgctcccag cagagaaggc ttaccacgca
      121 gcgcccattc accgaagatc cagccaagaa ttgccgccgc cgttgcgacc acagtattca
      181 caaatgccag tgccgcgatt tcattcgccg tgcccgctga cccggcgtta aagccaaacc
      241 aaccgatata gagaatggca gtcccggtga agaccatcgg caggttgtgc ggtttaaacg
      301 cctctttacc gaagcccacg cgtttgccta tcagatacgc gcccaccaga ccggcgattg
      361 ctgcgttaat gtgcaccacc gtgccacccg cgaaatccag cgcaccgtga gaagccagca
      421 aaccaccgcc ccacaccata tgcgcaatcg gaatgtaaga gagcgtcagc cataccacca
      481 cgaaaatcaa cacagctgag aagcggattc gttccgccag cgccccaact atcaagccta
      541 cggtaatgca ggcaaacgat ccctgaaacg ctacgtggat atactgataa atgctgccca
      601 tcaccgccgt cagttcgatg ttttttagca tcaaccagtt aatgttgccg aagaagttgt
      661 tgccctcacc aaacgccagc gaataaccgt aaaccaccca gagaatacag accagtgcaa
      721 acgtcaccgt cacctgcgtc agcatcgaca gcacgttttt gccgcgaatc aacccaccgt
      781 aaaacagggc aatccccgga atagtcataa acagcaccag cgcagtacaa atcatcataa
      841 acgcattgtc ggctttatcg gccaccgcag gtgcagccat taccagtccc ggcagcatcg
      901 ccagtgaagc aagcccagtt tttatcgtcg ctatcttcat ttttcgttcc tgttgctgtg
      961 tgccagagat tacagcgccg cttcgtcggc ttcgccggta cgaatacgaa tgacgcgttg
     1021 caattcagcg acgaagattt tgccgtcgcc aatttttccg gtgtaagccg ccttactgac
     1081 gatatcgatc acttcatcga gttggtcatc agcaatcgcc acatcaattt ttacttttgg
     1141 caggaagttg acgctgtatt ccgccccccg gtacagctcg gcatgccctt tctgacgccc
     1201 gaaacctttc acttcggtga cggtcaggcc ctgaatacca atggaagata acgcttcacg
     1261 aacgtcttcc agcttgaatg gtttgattat cacggtcacc agcttcatag atcccctccg
     1321 gtcagaattc ggtaatggtt ctgctacacg aagaaggtat tgcaagcggt gtgccagaaa
     1381 tgaaaaacga gaaagagagc aggaagttaa gaagattgaa aatgaaaacg cactatgaca
     1441 gtgcacagtg cgttacattt ttgcaccaac gatgaaatat tgcgctattc aggcgctcaa
     1501 tgattcctct tcacgcacgc tggctgccag ctcttcgccc gcaagttgca gttgatacat
     1561 ctgccagtag cgtccctggg ccgccagcag ttgctggtga gtgccctgct ccacggcttg
     1621 cccacgatga agcaccagaa tggtgtcggc atcaacaata gttgataagc ggtgagcaat
     1681 caccaccagc gtggtatgtt cacgcaccgc cgccagagca tgttgaatcg cctgttcagt
     1741 accggagtca atgctggcgg ttgcctcatc aaggatcagg atttgcggcg tctccaccag
     1801 cacgcgcgcc agtgccagca gttgcttttg cccgactgag agattatttc cctgctcgcc
     1861 cagcggcgta taaataccat cactcatgct gcgcgccagc tccgccagtt gcacggtttc
     1921 cagcgcctgc cagacgcgtt cttcggagat atcccgcccc agcgtcacgt tggcgaggaa
     1981 ggtatccgcc agcaccaccg gatcttgctg caccattgcc acgccctggc gcagcgcgct
     2041 atgacttagc gaacttaatg gacgaccatc aaggtgaatc tcaccttccg ttagcgggta
     2101 atagcccatc aataaactgg cgagggtact tttaccactg ccggtatgcc cgaccagcgc
     2161 cacaaagtta cgcgaaggca cagagagatt aatgttcttt agcaccagat tgtcatcgcg
     2221 ataagcaaat gacacgttat cgacttcgat ggtgccactc tgtaacgggt aatcatcatt
     2281 gccatattgc tggcgcggtc cgtccatcag ttcaaacacg cgctcaccag caacaacagc
     2341 ctgttgcagc atcgcctgtt gcgtggtcag ttcgattaag ggttcgttaa gtcgcccaag
     2401 atagctgata aacgcataca acacgcccac ttcaatggtg ccgctggcgg agaagccaaa
     2461 cagcatcaat aagccacaaa gaatgagcga tgaaaacaga ctcagcagcg gacgcagcag
     2521 aaaaccgtcg aggcgcaggg tttgcatcct cgccatatag tgtgaacgac tggcctcccc
     2581 catacgttcg ccaaatcgcg cctgctgacg aaactgctgg ataacgctca tgccattgat
     2641 gatttcgtta aagccgtcgt tgatatccgc cagataggcg cgcacacggc ggacaatcgg
     2701 cgtgctgtaa cgctggtata tcaccattac caccagcacc accgggaaaa tcattatcgc
     2761 caccagcgcc attcgccagt cgaggctgaa catcgccacc agcatcgcgc ccaccagtgc
     2821 ggcactgcgc aggacagttg ccactacggt aacgtagaga tcgcggatca cttcagtgtc
     2881 attagtgacg cgggaaatca cctgcccgac gggttgggta tcaaactcgc ttaatggctg
     2941 gcgtaacgca gcatccatca cgtcggtacg caactgttgc actacgccta ctgccgcccg
     3001 attaaacagc agcgactgcg cgtaatgtag cccggcggca aacagttgta gcccaacata
     3061 cgccgcagcc agccctgcaa ccactttcaa cggcaggtta tttttcgcta ccatattgtc
     3121 gataaaatag ctgataagca gcggcccact gacttctgcc gccgccgcaa cccacatcat
     3181 caggaccgca atccccagcg gtttacgcca cggcgaacca tacgctaaca ggcgcttgag
     3241 agtcggccac agttggctaa aactacgcat cgacggcctc ctcgcgaatt tccggaacgt
     3301 cgtcgagcgc cgcctccagt tgttgatagc gatacatatc gcgataccag ccgctttgtt
     3361 gcgccagctc atcatgattg ccacgctggg cgatatgtcc gtgctgcatc acaataattt
     3421 cactggcttc cgtcagtgcc gaaaggcgat gggcactgat gattaccgtt cttccctgtc
     3481 cccactgacg caggttatgc aggatctggt gctctgtgcg tccgtccacc gccgaaagcg
     3541 catcatcaag gatgaggatt tccgcgttga ctaataacgc tcgagcaatg gagatacgct
     3601 gtttttgccc accggaaagc atcacgccgc gctcgcccac ttctgtatcg taaccttgcg
     3661 gtagacgcaa aatatcgtca tgtacgctgg ctaaccgcgc gacatgctca atctcttgct
     3721 gggtggcatt cgggcaaccc agcgcgatgt tattcgccac ggtgtcagaa aaaaggaatg
     3781 gcgtctggct gaccaccgcc aggcggctac gccagctatc gagttgtaac ttcgtcagag
     3841 gaatatcatg aaagcgaata tccccctcgc tgacgtcgaa atgacgctga atgagcgaca
     3901 acagggtact tttgccggaa ccagtcggcc cgcaaatacc cagcatctga ccgggtttca
     3961 gggcgaaatt gacgttttcc agcgcaggat ggtcagtctg cggatacgtg aactggtgaa
     4021 tatttacatc cagttcgcca cgcccttccg gcacgggttc gctaccatcg ttcaccaccg
     4081 gcgcttccgc cagcatcgcg cgaatacggc tgtatgcagc actaccacgt tccacaatgt
     4141 taaacatcca tgccagcgcc agcattggcc aaatcatcag acctaaatac atcataaaac
     4201 tggtgagctg gcccagcgtt aaactgccct gcaccaccat ccaactaccg ccgccaatcg
     4261 ccagcaagtt cgccatacca atcgcgatat agatggtcgg gtcgaaacga gcatcaatac
     4321 gcgccacccg catgtttttt ttgccggtat cttcggcatc cgcggcaaat aacgccgact
     4381 ggcgatcttc cagaccaaag gctttgatca tgcggatact ggtgaggctt tcctgggtgc
     4441 ggtcattaag actggaaaac gccgcctgtg ccagcttaaa gcgttcatgc aaggcatcgc
     4501 cgttgcgctt aatcatgatc gccatcactg gcatcggtaa cagggcaaat aaggtcaact
     4561 gccagctaat ttgcgtagac atcataatca acacagcgca gcccattacc agtgaatcca
     4621 ccagcgtcag caccccttct ccggcggcaa acacgacgcg atcgacgtca ttggtcgcac
     4681 gagccatgag atcacccgtg cgatgacgca ggtaaaactc cgggtgctgc cggcttagct
     4741 gacggtaata atcttcacgc agttcaacag ccagttggta agacgcgcca aacagcaata
     4801 cgcgccagac ataacgcagg agataaacca caacggcaat cagcaccatg gtggcgatcc
     4861 acatcaggat cagcccggta gtaaagtgtt gttctgtcac gccatcgaca acaataccaa
     4921 ccacttttgg cggaaccagt tgcagcatcg cgataatgac aagcaaggcg acagccccaa
     4981 gatagcgacg ccattcccga cggaaatacc agcttaattg agcaaataat cgcacgcgtt
     5041 gtgtcctgac ctgattctgg atatttattc gatgggtaaa gaagtggtgt atttaatctg
     5101 ttccatcgcg aagctggaag tgacgtccga cagccccggc acgctgttta ccagacgctt
     5161 ataaaactcg tcgtagcgtt tcatgtcggc aacctggacg cgcatcagat aatcgtattc
     5221 accagccatg cgccagaacc ccagcacttc tggcatttcg gtaaccaccg tgacaaagcg
     5281 gcaataccat tcgctgctgt gatgttgcgt ttttatcagc acaaaggcgg tcaggccgag
     5341 gcctattttt tccggatcca gcagggcgac tttgccgata aggataccgt cgtcctccag
     5401 ccgtttcagg cgcttccagc agggggtggt tgtcagatta acggcttcag ccagtgcctg
     5461 caaagagagg gtgcaatcct gctgcagtaa ggccagcagc ttacggtcaa ttttatctaa
     5521 catagccctt ccacggagaa tttttttctc ttttaattct attttaaagg tgaaatcgcg
     5581 acaatttatt ctgtgcggtt taaacaccac ggcacaaaat gacaaattca acgcaaaaaa
     5641 aaaccgggca attgcccggt gctggaaggg atctcgttat tcgggggaat aaggtagatg
     5701 tggatagtcc agccagtgcg tcaaatagtg agagacagcc tgatttcggc aatgtccaat
     5761 caccggtaaa tgcgggagct ccgcgcgcag ttgcggcatc gcattgccca taataaaccc
     5821 gctaccgacg ctgcctaaca tttcgcgatc gttcatcgca tcaccaaagg ccatgcaatc
     5881 gcgcaacgat aaacctaaat gttgggtcag caccgtcaat gcagcgcctt tattgcagcc
     5941 caccggcagc acttcgaggc aatacgtggc ggaaaaacac aaatgtgcac gctcgcctaa
     6001 tgcttcgtat agctggatct gcaagcgtgt aagatcgtcg tgatcgccac agaagcagat
     6061 cttggtgacg ctgccgagtg gcattttttt gacatcgatt atctgataac gaaaaccgct
     6121 atagacaaat gcctgcaaca acgcagggat ctctttcccg gtaaaccaac cgtcgtcatt
     6181 gaagatatgc atgctggctc gggtatccca ttgctgatac agcaccagct ccgcgacatc
     6241 cgcaggtaaa tcatcacgat gtaaaagttc accttccaga gaatgcacgc gcgttccgtt
     6301 gccggtaatc aaatatgcat ccagcgatag cgccccgaga atatgctgca tctccagcgc
     6361 atgacgcccc gtggcaaaag tgagggtaat gtcgcgttca cgcagtcgcg ccaaagtaga
     6421 gagggttttc tcacctaaat gatggtcagg catcaataaa gtgccatcca tatcaaatgc
     6481 tgccagacga gccatttctt tctccactct gtgacacggt taatggtgat tgagtatcac
     6541 ctgatatata cggaagtaat agtgaatagt taaataatat tgttccgggt ttatatgcga
     6601 ttgctcaacc gtcttaacca gtatcaacgt ctgtggcaac cttcagccgg aaagccgcaa
     6661 accgtcaccg tcagcgaact ggccgaacgc tgtttttgca gcgaacgcca tgttcgtacg
     6721 ctgttgcgtc aggcacagga ggcgggatgg ctggagtggc aggcgcagtc aggacgcgga
     6781 aagcgcggac aattacgctt tctggtcacg ccggaatcgc tacgcaatgc gatgatggaa
     6841 caggcactgg aaaccggaaa gcagcaagat gtgctggagc tggcgcaact ggccccaagt
     6901 gagctgcgca ctctgttaca gccgtttatg ggcggacaat ggcaaaacga tacacccacg
     6961 ttgcgtattc cctactatcg cccgctcgaa ccgctacaac caggcttttt gcccggccgt
     7021 gccgagcagc atctcgccgg gcagatattt tccggcctga cccgcttcga taataatact
     7081 cagcgcccga ttggcgattt agcgcatcac tgggaaacct ctactgacgg gttacgctgg
     7141 gacttttatc ttcgttcaac cctacactgg cataacggcg atgcagtaaa agcctcacac
     7201 ttacaccagc gattattgat gctgttacaa ctgccagcac tggatcaatt atttattagc
     7261 gtgaagcgta ttgaagtcac ccatccgcag tgtctgacct tctttttaca tcgccccgat
     7321 tactggcttg cgcaccggct ggcgagctat tgcagccatc tggcgcatcc gcaattccca
     7381 ctgatcggca ctggtccttt tcgcttaaca caattcacag cggagctggt gcgcctggag
     7441 agccatgatt attaccattt gcgtcatccg ctgcttaaag cggttgagta ctggataact
     7501 ccgccgcttt tcgaaaaaga tttgggaacc agttgtcggc atcccgtgca aatcaccatc
     7561 ggcaaaccgg aggagctgca acgggtcagc caggtcagta gtggcatcag tttaggtttt
     7621 tgctatttaa cgttgcgcaa aagtccccga ctctccctct ggcaggcgcg aaaagtgatc
     7681 tccattattc atcaatccgg tttattacaa acgttagaag tcggagaaaa cctgatcacc
     7741 gccagtcatg cattactgcc aggctggact attccgcact ggcaagtacc ggatgaagtc
     7801 aaactaccga aaaccttgac gctggtttat cacctaccga tagaacttca taccatggca
     7861 gaacgcctac aggcgacact ggcagcggaa ggctgtgaac tcacaattat ttttcataac
     7921 gcaaaaaact gggacgacac gaccctactg gcacacgcag acctcatgat gggcgacaga
     7981 ttaattggcg aagcaccgga atatactctg gagcaatggc tacgttgcga tccactatgg
     8041 ccacatgttt tcgacgctcc agcatatgca catctgcaat cgacgctgga cgcggtgcaa
     8101 gtaatgcctg atgaggaaaa ccgatttaat gccctgaaag cggtttttag ccagttaatg
     8161 gcagacgcga cgctgacgcc gctgttcaac tatcactatc gcattagtgc ccctcccggc
     8221 gtgaacggtg tgcgactgac accgcgcggc tggtttgaat ttaccgaagc ctggcttccc
     8281 gcgccgtcgc aatgaatagc tggtccgggt taatcgcagg cgttaccata acgcttttat
     8341 tgttcaagca ggattatcta tgaaacgtgc tgtcgttgtg ttcagtggag gccaggattc
     8401 caccacctgt ctggtgcagg cattacaaca atatgatgaa gtccattgcg tgacgttcga
     8461 ttacggtcag cgacatcgcg cagaaatcga cgtggcacgc gaactggcgc tgaaactggg
     8521 agcacgcgcg cataaggttc ttgatgtaac gctgctcaac gagctggcgg tcagcagcct
     8581 gacacgtgac agcattccgg tgcctgatta tgaacctgaa gccgatggca tcccgaatac
     8641 gtttgtccca gggcgtaata ttttgttcct gacgctggcg gcaatatatg cgtatcaggt
     8701 aaaagcagaa gccgtaatta ccggcgtctg tgaaacggat ttctccggct acccggattg
     8761 ccgcgatgag tttgtgaaag cactaaacca tgccgtcagt ttgggcatgg cgaaagatat
     8821 tcgttttgaa acgccgctga tgtggattga taaagcggaa acctgggcgc tggcagatta
     8881 ttacggcaaa ctggatttag tccgtaacga aacgttgacc tgctataacg gcattaaagg
     8941 cgacggttgc ggtcattgtg cggcatgtaa tttacgcgcc aacggtttga atcattatct
     9001 ggccgataaa ccgacggtga tggcagcgat gaagcagaaa accgggttga ggtaattatt
     9061 ccgggtgtcg ccggatgcgg cttgagcatc cggcaccaca aaacgtttac ttaaccatct
     9121 gctccagctt ttcgcgcaat tccccttcca gagctaatgc tttctgcgtt ttaagatcaa
     9181 tacaaacaaa cgtaataagc gcatccgcta ccacctgccc ttccggctcc agtgtaatga
     9241 cctggcttaa gatgccgctt ttaccgttta attgctgcaa ctgactggta atagttaaca
     9301 ggtcgcttaa taccgctggg cgacgatagt taatattgat attgaccacg acgaaggcga
     9361 tgttatgggc cgtcatccac tgaaaactgt cgctattttc caacccatcc cagcgggctt
     9421 cttcgagaaa ttcaaggtag cgggcgttgt tgacgtgctg gtaaacgtcg agatgatatc
     9481 cacgaacttt gatttgtgtt tgcatagcgc aataacctta cgttgttgtt ataagtacag
     9541 aggtcataac tggtatgacc tcttcagtct ggcaaaaaat tgccactatg caaattaatt
     9601 acagggttaa taccgccaga ttacgttcca ccagcgaatt gcccatcccc ggcacctgct
     9661 ttagatcctc caccgtttta aacggaccgt actcttcgcg ataactgaca atcgcctgcg
     9721 ctttcttcag gccaacgcca ttcatcgcgc gggctagctc ttccgcgctg gcattattaa
     9781 tgctgacccg ggtgccttct tcgtcactgg ctttcgccgg tactgctgct ttactttgtg
     9841 ctgcaggagc ttccgctttg gtttctaccg ccgtcggttt cgccacagaa gctgccgcca
     9901 gcgcgctatg agacattccg gcacaggcca gggacagggt aatgagcagt gctttaattc
     9961 cgtgtttcat actgttttct ccttgtttgt taacagtgga atcaccttaa cgggagtgaa
    10021 aacagtaaac aaatggcaat gttcagaaat ggaaaaggcc gcgaaagcga ccttttcttg
    10081 ttacagctgc aacgcatttt tttgcgaggc ggcttcagga ttattgctgt tccagcgcat
    10141 cgccaatttt gattttcgcc tctttacgca ggttactcat cagagcttca aagacgattt
    10201 gtgcgttgtt ctgggtgata ccctgcacca tcgctttttt ctgatcttcc ggcattgaac
    10261 cttgtttcac ttcatccagc gccagcagaa ccacattacc ttgcatatcg gtcgccatac
    10321 cgtagctcgg tttgtctttc gctggcagtg gcagtgcaaa cgccgcctgg ctaatcgggt
    10381 cacgaccgga acggcttaag gttttcggct cgccaaattt cagaccggca gcctgcatag
    10441 cttccgcacc tttgccggct ttcaaatcaa ccagcagttt ctcagcatcc actttcgcct
    10501 gttgttcagc tttgttgtgc tgaaccagtg ccttaacttg ttcctgaaca tctgccaacg
    10561 gtttcaccgc ttccggtttg tgctcgctga tgcgcagcac gaatgcgcgg tcgccgtcta
    10621 cggtgatgat gtcagagttg atgcccggcg cgccgttttc acctaccaga ccgccgttaa
    10681 agatagcgtc ggcaaccggc ttgaagttca actcttccgg caggttatct ttgctgaacc
    10741 aacccgtctg agtggcttta acgccggcag cttgctctgc accggccaga gactcggtgt
    10801 cgttgcttgc cgcatcgctc actttctgct gcagcgcata gtacgcatcg agggcttttt
    10861 cgtgtttcac tttcgccgca atgtcgtcac gtacttcgtc taacgatttc actttcgctg
    10921 gctgaatgtc gtccagacgt acaatcagga aaccaaccga agatttgatg acaccagaca
    10981 gttggccttt ttctttcaga ccagcatttt tcagttcgtc ggggatagtg gcatcttcta
    11041 accaacccat atcgccgccg ttacgggcag agataatatc ggcagatttt tctttggcta
    11101 atgcagcaaa atcaccgcct ttattcagct catcaagtac cgctttcgct tcatcttcag
    11161 ttttggtctg gatgatgctg tagcgggtac gctgcggctg ggtgaattga tcctgatgct
    11221 gatcgtagta gctctggata tccgcatcgc taaccggttg ctgcatcgtt gcggcaacca
    11281 gcttgatgta actcacgcgg aattgttccg gcgtcatgaa attgtttttg ttttgttcgt
    11341 agtagctggc aatctcctgt tcggtcacag gctgcttcgc cgccagcgcg ttaacatcga
    11401 tagtcgcctc acgcaccacg cgttgttgcg cgaccagtgc cgccagctcg tcggtttcac
    11461 ctttcagcat aaaatcggta ccggcaacgc cgttaatcag ctgttgggta gtgagctggt
    11521 tacgcagcgc ctgggcgtac tgatcggcgg tcatccccat ctggttgagg ataccgttat
    11581 agcggctgtt atcaaatttg ccgtcaacct ggaaggctgg ggtcgcgaaa atcgcctgtt
    11641 taacctgctc atcactgata cccagtttca gctcacgagc gtactgatcc aacagcgcct
    11701 cgtcgatcag acgattcagc acctgttgac gcagggtttt catatagcct tcgttcgctg
    11761 ccagctcgga gtattgatcg cccagctgtt gctgcatgcg attacgctcg ctgttaaagg
    11821 cgttctcgaa ctgcccacgg ctgatttcct ggtcattcac ttttgcggcg tagttattgc
    11881 ctccgccaat caggtaacca ctcacgccgg tcaatatgaa cgacacgata atgataccga
    11941 aaataatctt gagcacgaga ctgtttgcag ccgtgcgtaa gctgtccatc atggtgtaac
    12001 aacactccgc tgtagatgac tgtgtacctc acgcaacgta tcgatgcgcg caaaggccta
    12061 ttgtgacaag aaacgggggc aattgtcagc ccacaagaca cagaaagtca ccgaatacaa
    12121 ataaaaaagg cacatcagta gatgcgccct tgaacttcgt cacatcccca ctggggacaa
    12181 cgcttagttt accgcgtctt tcagtgcttt acctgcacgg aagctcggta ctttagcggc
    12241 agcgatggtg atctctttac cggtctgcgg gttgcggcca gtacgggcag cacgctcttt
    12301 aacggcaaaa gtaccaaaac ctaccagtgc tacatcatcc ccttctttca gagattcagt
    12361 tacggaagca ataatagcat ctaacgcacg gccagccgca gctttagaga tatcagcccc
    12421 tgcagcaatc ttgtcgatca attgagattt attcactctt ctcttcctct ttataattta
    12481 tatcgcactt gaatccttca aggtacgaac gcgcagcagt tatatcaggc cagccatccc
    12541 cttacaacac cccttaataa gggcaagccc gatccgctat ctaacttagc gagacaaaaa
    12601 aaggctggca agcccgaatt agcctgccag ccctgttttt attagtgcat tttgcgcgag
    12661 gtcactattt tgcagtcaca acctgcatgc cagacggttc attttgcagc gccagagtca
    12721 gaacttcctc aatgcgcttc acaggatgaa tgtccagatc ggcaattacg ttgtcaggaa
    12781 tctcttccag atcgcgttta ttttcgaacg gaattagcac tgttttaatc ccgccgcgat
    12841 gcgctgccag gagtttttct ttcaaaccac cgatcggcag tacctgacca cgcagagtga
    12901 tctcaccggt cattgccaca tcggcacgaa ccgggttacc ggtcaggcaa gaaaccagcg
    12961 cggtgcacat agcaataccg gcactcggac catctttcgg cgtcgcacct tccggtacgt
    13021 ggacgtggat gtcacgtttt tcgtaaaaat cagggttgat ccccagtttt tccgcacgcg
    13081 cacgaaccac cgttaacgcc gcctgaatag actcctgcat cacttcgccg agcgaaccgg
    13141 tataggtcag tttgccttta cccggaacac atgcggtttc aatggtcagc aagtcaccgc
    13201 ccacttccgt ccacgccaga ccggttacct gaccgacacg gttttcgtta tcagcgcgac
    13261 catagtcgaa acgctgaaca ccgaggtagt catgcaggtt atcgccgtta atttcgatat
    13321 gttttaatga attatcgagc agtaactgct taaccgcttt gcggcacagt ttggagattt
    13381 cacgctccag accacgcacg cccgcctcac gggtgtagta acgaataatg ccgataatgg
    13441 cgctatcgtc gacggtcagc tcaccttttt tcagtgcatt acgttcaatc tgcttcggca
    13501 gcaggtgacg tttggcgatg ttcagttttt catcttcggt ataaccggag aggcgaatca
    13561 cttccatacg atccagcagc ggtgccggaa tgttcatgga gttcgacgtc gcgacaaaca
    13621 tcacgtcgct gagatcgtaa tccacttcca ggtagtggtc gctgaacgct acgttctgct
    13681 ctggatccag cacttcaagc agtgcagagg ccggatcgcc tcgcatgtca gaagacattt
    13741 tgtcgatctc atcgagcagg aacagcgggt ttttcacgcc cactttcgcc attttctgga
    13801 tcagtttacc cggcatagaa ccgatgtaag tacggcggtg accacggatt tccgcttcat
    13861 cacgcacgcc gcccagcgcc atacggacat atttacgccc ggtggctttg gcaatggact
    13921 gaccaagaga ggttttacct acccccggcg gccctaccag gcagaggatc ggtcccttga
    13981 ttttgttgac acggctttga accgcaaggt actcaaggat gcgatctttc acgcgctcca
    14041 gaccataatg gtcggtatca aggatttcct gcgcctgacg caggtctttt ttgaccttgc
    14101 tgcgcgcatt ccacggtacc tgtaccatcc agtcgatata accacgcact acggtcgctt
    14161 ctgccgacat cggagacatc attttcagct tctgcaactc tgcttccgct ttctcttttg
    14221 cctctttcgg catcttcgcc gcgtcgattt tgcgcttcag ggcttcgttt tcgtccggcg
    14281 cgtcgtccat ttcaccgagt tctttctgaa tagctttcat ttgctcgttc agatagtact
    14341 cacgctggga tttctccatc tgctttttaa cgcggttgcg aatgcgtttc tcaacctgca
    14401 gcagatcgat ttccgattcc atcattgcca tcagatattc cagacgttcg ttaacgtcgg
    14461 acatctccag aacggactgt ttgtcagcca gtttcagcgg catatgtgca gcaatggtat
    14521 ccgccagacg cgccggatcg tcgatgctat tcagcgacgt cagcacttct ggtgggattt
    14581 ttttgttcag cttgatgtag ccttcgaact ggctgattgc agtacgcacc agcacttcct
    14641 gttcccgctc atcaatggtc ggcgactcca gatactccgc cttcgcagaa aagtgttcgc
    14701 cattgtcaga gagcgcagaa atacgcgcgc gctgtaaccc ctcgaccagc actttgacgg
    14761 tgccgtcagg cagtttcagc atctgcaata tagaggccac ggtcccgacg gtgaaaagat
    14821 cgtttacacc cggctcatcc gttgaagctt ctttctgcgc gaccagcata atttttttat
    14881 catggtccat cgccgcttcc agacaacgga tagatttttc ccgcccgaca aataagggga
    14941 tgaccatgtg cggataaacc accacatcgc gcagcggcaa tacggggatt tcaatgcgtt
    15001 cagaacgctc aggattcata gagctctctc ttagtttaat ttccgccagg taatcagatg
    15061 acacgactgt gcttcacgcc atctattaac atgtacgtca gtatatgggg atgtttcccc
    15121 cacattcaac gccgagaata gaggaaaaat taaaggggag ataaaatccc ccctttttgg
    15181 ttaactaatt gtatgggaat ggttaattat tcaccagatg cctgttgcgc ttccggctta
    15241 ccataaatca gcaacggttt gctttgacca tcaattaccg actcgtcgat aaccactttt
    15301 tcgacgtctt ccatggacgg cagatcgtac atggtatcga gcagtgcggc ttctacgatg
    15361 gaacgcaggc cacgggcacc ggttttacgc gccatcgctt tcttagcgat agcatccagc
    15421 gcctcgtcac ggaattccag atccacgcct tccagattaa acagcgcctg atactgcttg
    15481 gtcagggcgt ttttcggctc tttgaggatc tgaatcagag cttcttcgct cagttcattc
    15541 aacgttgcta caaccggcag acgaccaatg aactcaggga taagaccaaa cttgatcaga
    15601 tcttccggtt caacctgcgc cagcagctcg ccttcgcttg ctttgtcgga cttcgctttt
    15661 accgtcgcgc caaaaccaat gccggagccg gtttctacac ggtgggaaat cactttatcc
    15721 agaccggcaa acgcaccgcc acaaataaac aggatcttag aggtatcaac ctgcaagaat
    15781 tcctgctgcg gatgtttacg cccaccttgc ggtggaacag cagctaccgt accttcgatc
    15841 agtttcaaca gtgcctgctg tactccttca ccggaaacgt ctcgggtaat ggacgggttg
    15901 tctgacttac gagaaatctt gtcgatttca tcgatgtaga caatcccgcg ctgcgctttc
    15961 tgtacgtcgt aatcgcactt ttgcaacagc ttctgaatga tgttttcaac gtcttcgccc
    16021 acataaccgg cttcggtcag cgtggttgcg tcggccatgg tgaacgggac gtccagcagg
    16081 cgcgccagcg tttcagccag cagcgtttta ccggaaccgg tcggaccgat cagcagaatg
    16141 ttacttttgc ccaactcgac accattgctg gtatcgccgt tgcgcagacg tttgtaatgg
    16201 ttgtataccg cgaccgccag cacttttttc gcctgttcct ggccgataac gtaatcgtcc
    16261 aggtggttgc gaatttcatg cggcgtcggt agcgcactgc gttcacgatg cggtgcaact
    16321 tctttaatct cttcgcgaat gatgtcgtta cataaatcaa cacattcgtc gcagatatac
    16381 acggatggac cggcaatcag cttgcgcact tcatgctggc ttttgccgca aaaagagcaa
    16441 tacagcaatt tgcctgagcc atctttgcgt ttatctgtca tgggtcaaaa cctcttcttt
    16501 gttctttgtg ccgcacacga cgacgcaaat gccattctca ggcgcaagcc gcttacagcg
    16561 ttgtgccgcc ctagataagt atagcggcac agttgcgcct ctggcatcaa ttacgatggg
    16621 tcagaatcga atcgaccaga ccgtattcca ccgcttcagg ggcggaaagg aagcgatcgc
    16681 gctcggtatc acgttcaatc tgttctaatg attgacccgt atgaagcgcc ataagttcat
    16741 tcatgcgccc tttaactttc agaatttcac gggcatgaat ttcgatatcg gtcgcctggc
    16801 cctggtagcc gcccaacggt tggtgaatca tcacgcgcga attcggcagg caaaaacgtt
    16861 taccttttgc ccctgcggtc aacaagaaag cgcccatcga ggccgcctgg cccatacaga
    16921 tggtgctgac atcaggcttg ataaactgca tggtgtcata gatagacatc ccggcagtga
    16981 tcaccccgcc tggggagtta atgtacagat agatatcttt ttctgggttt tccgcttcca
    17041 ggaacagcat ctgcgccaca atcaggttag ccatgtggtc ttcaacctgg ccagtcagaa
    17101 aaatgacgcg ttccttaagt agacgagaat agatatcaaa agagcgctca ccgcgtgagg
    17161 tctgttcaat gaccatcggc accagcgcca tatggggtgc aaagttatct cgttcgccgc
    17221 tgtatgacat ttccgtctcc tggataaaat tgaaaaaaac ctgctgtacc gattgtaacc
    17281 ttatggacgg attatcagct agtccctatg ttatgggtac ggcatcacca tatttcaagc
    17341 ataacaatct tttgttgtta cgctaacacc gaaagaggct tttcgcacgg ctccatgcaa
    17401 aattcatgac aaaaaaaagc ccaccacctg gcggtgacgg gcctttgtgc gaatttagcg
    17461 cgttatgctg cgtaaattac gcctgctggt tcatcagctc gttgaaagtg gtttcttttt
    17521 cagtcacttt cgctttcgcc agtacagctt caacagcctg ttcttccaga gcaacattgc
    17581 gcatgttgtc catcagttct ttgtttttgc tgtagaactc gataacttct ttcggatctt
    17641 cgtacgcaga agccatctct tcgatcaggc ctttcacgcg ctcttcatca gctttcagct
    17701 cgttggtgcg gataacttcg cccagcagca ggccaacaac tacgcggcgt ttagcctgtt
    17761 cttcgaacag ttcgcgcggc agttccagag cttgtttttc gttgccaccg aaacgctgtg
    17821 cagcctggcg acgcagaacg tcgatttcgc tgtcgatcag cgcagccggt acgtcgatgt
    17881 cgttagcttt taccagacct tcgatcgcct gagacttaac gcggttacgg atggcgctct
    17941 tcagctcgcg ctccatattt ttacgcactt cagcgcgcag accttctacg gaaccatctt
    18001 caacgccgaa acgtttgatg aattctgcag tcagttccgg cagttcacgc tcttcaactt
    18061 tcttcaggtt gatagcgaat ttcgctgctt tacctttcag gttttctgcg tggtattctt
    18121 ccgggaaggt cacgtcgatg gtgaactctt cgccagcttt gtggcctttg ataccgtctt
    18181 caaagcccgg gatcatacga ccctggccca tcgccagtac gaaatcagac gctttaccgc
    18241 cttcgaactc ttcgccgtct acagaaccgg tgaagtcgat ggttacgcgg tcttctgctt
    18301 caacagcgcc gtctttttct ttccaggtcg cctgctgttt acgcagagta tccagcatgc
    18361 cgtcaacgtc agcgtcggtc acttcaacga tcggtttttc aacttcgatc gcttccagac
    18421 cctgcagttc aacttccgga taaacttcaa actctacaga gtaagtgaag tcttcaccca
    18481 gcttgtattc gcccggaaca taagtcggtg cgccagccgg attgattttt tctttaatga
    18541 tggcgtcaat gaagttacgg ctcatcaggt cacccagaac gtcctggcgt acagacgcgc
    18601 cataacgctg agcaacgata ttcattggca ctttgccttt gcggaagccg tcaatacgta
    18661 cttttttcgc aacgttgacc agctcgcttt taacagcggt ctcgatgctg tcagcagcga
    18721 tagtaatcgt tacacggcgg ccaaggcctt gagtggtttc aactgaaact tgcatcttgt
    18781 tacctcaaaa aatcacagtg ctcggtcaac tcttaccccg caagcacagg ttgttggctt
    18841 cgcggaaccg ggatgttctt ataatcaatc acattccctg ttaccagaat catcccgaaa
    18901 gcattcaaat aggacgcggc attatagcgg catcactttt atgagtcgag aacggttatt
    18961 gcgcgctgtc gcgtattttt tcacaattcc cgggcaattt tcgtctgaat gctgggcaaa
    19021 aagcgcaaaa tccagacaaa acaaaacggc ccgcaggccg tttttcataa aatcttcagc
    19081 aacatactgg aaaagcgccg acagttgcaa atgcgttttt acgcgatgct tcctgctcca
    19141 cgacagggag gagaggcaaa gacggtgtcc tgcaaccctt cccactcctt aatagtgtaa
    19201 gtatgcagag ccagcgcatg aacggtagta gagagttcct ccgctaaagt actgtaaatc
    19261 attcgatgac gattcagaaa acgttcaccc gtaaaacgat cgctgaccag cacaacttta
    19321 aaatggcttt cagagccggc tgggacattg tgacgatagc tttcatccac tacttcgagg
    19381 aatacgggtt ggaacgccgc ccttaatttt tcttctatcc gctcacgtat catcatgaaa
    19441 tatctcctcc gacaacgctg agatgtcatc catcccttta aatactagcc gcttttaccg
    19501 tttccattgc agcttaacaa caaatattta ggtttcgtcc tggtttttcc gtcaagcgta
    19561 tgaagtttac tgatttaaag attgaaacac aggcctcgca tcagcgtttc ttttaccagg
    19621 ggtgattatt tcgtcaacat gatgaattta catgccttac ccacttcccc tcgccgttgg
    19681 caatgttatg atggcgggaa tttttaccaa acacgcttaa gagtctctga gagcccgaac
    19741 atgttcaaaa aaatcctctt cccgttagtt gctctgttta tgcttgcagg atgcgcaaaa
    19801 ccgccaacaa ctattgaagt ttccccgacg attacactgc cacagcagga tccaagcctg
    19861 atgggcgtca ccgtaagcat taatggtgcc gatcagcgta ccgatcaggc gctggcaaaa
    19921 gtcacccgcg ataatcaaat cgttaccctg accgcctccc gcgatctgcg tttcctgctg
    19981 caagaagtgc tggaaaaaca gatgaccgcg cgtggttaca tggttggtcc gaatggcccg
    20041 gttaatctgc aaatcatcgt tagccaactg tatgctgacg tgtcccaggg caacgtgcgc
    20101 tacaacatcg ctaccaaagc ggatatcgcc atcatcgcta ccgcgcagaa tggcaacaaa
    20161 atgaccaaaa actatcgtgc cagctacaac gttgaaggtg cgttccaggc ctccaacaaa
    20221 aatatcgccg atgcggttaa cagtgtgctg accgatacca tcgctgatat gtctcaggac
    20281 accagcattc acgaattcat caagcagaac gcgcgttaat ttctgccctc tggcccggtg
    20341 caagccgggc ctgtagacgc ccatgtccag tcaatattta cgtatttttc aacagccgcg
    20401 ttcagccata ttgctgatcc tgggttttgc ttccgggcta ccgctcgccc tgacatccgg
    20461 caccttacag gcctggatga cggtcgagaa tatcgatctc aaaaccattg gtttcttctc
    20521 tctggtaggc caggcttacg tttttaaatt cctctggtca ccgctgatgg accgctacac
    20581 gcccccattt tttgggcggc ggcgcggttg gctgctcgcc acgcaaatcc tgttattagt
    20641 cgccattgcg gcgatgggtt ttctcgaacc aggcacccaa ctccgctgga tggcggcgct
    20701 ggcggtggtg atcgcttttt gctctgcctc ccaggatatt gtcttcgatg cgtggaaaac
    20761 cgatgtgctc ccggcagaag aacgtggtgc gggcgcggca atcagcgtgc tgggttaccg
    20821 tttagggatg ctggtttccg gcggcctagc cctgtggctg gcagataaat ggttgggctg
    20881 gcagggcatg tactggttga tggcggcgct gttgatcccc tgtattatcg cgacgttgct
    20941 tgcaccagaa ccaaccgaca ccattcctgt gcccaaaacg ctggaacaag cggttgttgc
    21001 acctctgcga gatttctttg gtcgcaataa tgcctggctt attttgcttc ttatcgtgct
    21061 gtataagctg ggcgacgcat tcgccatgag cctgacaacc acgtttttga ttcgcggcgt
    21121 cgggtttgat gcgggtgaag taggcgtggt aaacaaaacg cttggcttac tggcgaccat
    21181 tgttggcgca ttgtacggtg ggattttgat gcagcgcctg tcgctgttcc gggcgctgct
    21241 gattttcggc attttacaag gtgcgtctaa cgctggttac tggctgctgt cgattactga
    21301 taagcatctc tacagcatgg gcgcagccgt ctttttcgaa aacctctgtg gcgggatggg
    21361 cacatcagcc tttgtcgcgc tgttaatgac gctgtgtaat aagtcatttt ccgctactca
    21421 atttgccctg ctctcagcgc tttccgctgt agggcgagtt tatgtcggcc ccgtggcggg
    21481 ttggtttgtt gaagcacacg gctggtcgac attctatcta ttctccgtcg ccgctgccgt
    21541 accggggctt attttgctgc tggtttgccg ccagacgctt gaatatacac gagtaaatga
    21601 caactttatc tcccgtaccg aatatccggc aggttatgcc tttgccatgt ggacactggc
    21661 ggcgggcgtc agcctgttgg ccgtgtggtt acttctgttg acgatggacg cgctggattt
    21721 gacgcacttc tctttcctgc ctgctctgct ggaagtcggg gttttagtcg ccctttctgg
    21781 cgtcgtgctt ggtggtttgc tggattatct ggcgctacga aaaacgcatc tgacgtaatc
    21841 tgtaaatatt atttagctgt agtaatcact cgccgtaatt attacggcta aataaacatc
    21901 agcatcgctt attaatattt gttcttttgt gcggcttagc gtttggaaat tattggcgcc
    21961 atttataaat tctatttttc ttacacgatt cagctaatga gtctttattt tctcaccacc
    22021 cagttgtcac tctaatgata attatttgtt aaataattgt tttatttcgc attggttata
    22081 ccaattgccc gccaagactc cttctgcact ccccttttgt tataacgccc ttttgcaaca
    22141 gcttcttaaa atcaacctga tatgttttgc aacatatgtg acctgacagc caaatctaag
    22201 taacaggaat ttaaccatgt ctacagtaat gtaacctccc cgtaaaatgc ccacacactt
    22261 taaacgccac cagatcccgt ggaattgagg tcgttaaatg agactcagga aatacaataa
    22321 aagtttggga tggttgtcat tatttgcagg cactgtattg ctcagtggct gtaattctgc
    22381 gctgttagat cccaaaggac agattggtct ggagcaacgt tcactgatac tgacggcatt
    22441 tggcctgatg ttgattgtcg ttattcccgc aatcttgatg gctgttggtt tcgcctggaa
    22501 gtaccgtgcg agcaataaag atgctaagta cagcccgaac tggtcacact ccaataaagt
    22561 ggaagctgtg gtctggacgg tgcctatctt aatcatcatc ttccttgcgg tactgaccta
    22621 gaaaaccact cacgctcttg agcctagcaa gccgctggca cacgacgaga agcccattac
    22681 catcgaagtg gtttccatgg actggaaatg gttcttcatc tacccggaac agggcattgc
    22741 aaccgtgaat gaaatcgctt tcccggcgaa cactccggtg tacttcaaag tgacctccaa
    22801 ctccgtgatg aactccttct tcattccgcg tctgggtagc cagatttatg ccatggccgg
    22861 tatgcagact cgcctgcatc tgatcgccaa cgaacccggt acttatgacg gtatatccgc
    22921 cagctacagc gggccgggct tctcaggcat gaagttcaaa gctattgcaa caccggatcg
    22981 cgccgaattc gaccaatggg tcgcaaaagc gaaacagtcg ccgaacacca tgtctgacat
    23041 ggctgcgttc gaaaaactgg ccgcgcctag cgaatacaac caggtggaat atttctccaa
    23101 cgtgaaacca gacttgtttg ccgatgtgat taacaagttt atggctcacg gtaagagcat
    23161 ggacatgacc cagccagaag gtgagcacag tgcacacgaa ggtatggaag gcatggacat
    23221 gagccacgcg gaatccgccc attaaagggg ttgaggaaga ataaagatgt tcggaaaatt
    23281 atcacttgat gcagtcccgt tccatgaacc tatcgtcatg gttacgatcg ctggcattat
    23341 tttgggaggt ctggcgctcg ttggcctgat cacttacttc ggtaagtgga cctacctgtg
    23401 gaaagagtgg ctgacctccg tcgaccataa acgcctcggt atcatgtata tcatcgtggc
    23461 gattgtgatg ttgctgcgtg gttttgctga cgccattatg atgcgtagcc agcaggctct
    23521 tgcctcggcg ggtgaagcgg gcttcctgcc acctcaccac tacgatcaga tcttcaccgc
    23581 gcacggcgtg attatgatct tcttcgtggc gatgcctttc gttatcggtc tgatgaacct
    23641 ggtggttccg ctgcagatcg gcgcgcgtga cgttgcgttc ccgttcctca acaacttaag
    23701 cttctggttt actgttgttg gtgtgattct ggttaacgtt tctctcggcg tgggcgaatt
    23761 tgcgcagacc ggctggctgg cctatccacc gctatcggga atagagtaca gtccgggagt
    23821 cggtgttgat tactggatat ggagtctcca gctatccggt ataggtacga cgcttaccgg
    23881 tatcaacttc ttcgttacca ttctgaagat gcgcgcaccg ggcatgacca tgttcaagat
    23941 gccagtattt acctgggcat cactgtgcgc gaacgtactg attattgctt ccttcccaat
    24001 tctgacggtt accgtcgcgt tgttgaccct ggatcgctat ctgggcaccc atttctttac
    24061 caacgatatg ggtggcaaca tgatgatgta catcaacctg atttgggcct ggggccaccc
    24121 ggaagtttac atcctgatcc tgcctgtttt cggtgtgttc tccgaaattg cggcaacctt
    24181 ctcgcgtaaa cgtctgtttg gttatacctc gctggtatgg gcaaccgtct gcatcaccgt
    24241 gctgtcgttc atcgtttggc tgcaccactt ctttacgatg ggtgcgggcg cgaacgtaaa
    24301 cgccttcttt ggtatcacca caatgattat cgccatcccg accggggtga agatcttcaa
    24361 ctggctgttc accatgtatc agggccgcat cgtgttccat tctgcgatgc tgtggactat
    24421 cggttttatc gtcaccttct cggtgggcgg tatgactggc gtgctgctgg cagtaccggg
    24481 cgcggacttc gttctgcata acagcctgtt cctgattgcg cacttccata acgtgatcat
    24541 cggcggcgtg gtcttcggct gcttcgcagg gatgacctac tggtggccta aagcgttcgg
    24601 tttcaaactg aacgaaacct ggggtaaacg cgcgttctgg ttctggatca tcggcttctt
    24661 cgttgccttt atgccgctgt atgcgctggg cttcatgggc atgacccgtc gtttgagcca
    24721 gcagattgac ccgcagttcc acaccatgct gatgattgca gccagcggtg cagtactgat
    24781 tgcgctgggt attctctgcc tcgttattca gatgtacgtt tctattcgcg accgcgacca
    24841 gaactgtgac ctgactggcg acccgtgggg tggccgtacg ctggagtggg caacctcttc
    24901 cccgcctccg ttctataact ttgccgtagt gccgcacgtt cacgaacgtg atgcattctg
    24961 ggaaatgaaa gagaaaggcg aagcgtataa aaagcctgac cactatgaag aaattcatat
    25021 gccgaaaaac agcggtgcag gtatcgtcat tgcagctttc tccaccatct tcggtttcgc
    25081 catgatctgg catatctggt ggctggcgat tgttggcttc gcaggcatga tcatcacctg
    25141 gatcgtgaaa agcttcgacg aggacgtgga ttactacgtg ccggtggcag aaatcgaaaa
    25201 actggaaaac cagcatttcg atgagattac taaggcaggg ctgaaaaatg gcaactgata
    25261 ctttgacgca cgcgactgcc cacgcgcacg aacacgggca ccacgatgca ggcggaacca
    25321 aaatcttcgg attttggatc tacctgatga gcgactgcat tctgttctca atcttgtttg
    25381 ctacctatgc cgttctggtg aacggcaccg caggcggccc gacaggtaag gacattttcg
    25441 aactgccgtt cgttctggtt gaaactttct tgctgttgtt cagctccatc acctacggca
    25501 tggcggctat cgccatgtac aaaaacaaca aaagccaggt tatctcctgg ctggcgttga
    25561 cctggttgtt tggtgccgga tttatcggga tgaaaatcta tgaattccat cacctgattg
    25621 ttaacggcat gggtccggat cgcagcggct tcctgtcagc gttctttgcg ctggtcggca
    25681 cgcacggtct gcacgtcact tctggtctta tctggatggc ggtgctgatg gtgcaaatcg
    25741 cccgtcgcgg cctgaccagc actaaccgta cccgcatcat gtgcctgagc ctgttctggc
    25801 acttcctgga tgtggtttgg atctgtgtgt tcactgttgt ttatctgatg ggggcgatgt
    25861 aatgagtcat tctaccgatc acagcggcgc gtcccatggc agcgtaaaaa cctacatgac
    25921 aggctttatc ctgtcgatca ttctgacggt gattccgttc tggatggtga tgacaggggc
    25981 tgcctctccg gccgtaattc tgggaacaat cctggcaatg gcagtggtac aggttctggt
    26041 gcatctggtg tgcttcctgc acatgaatac caaatcagat gaaggctgga acatgacggc
    26101 gtttgtcttc accgtgctaa tcatcgctat cctggttgta ggctccatct ggattatgtg
    26161 gaacctcaac tacaacatga tgatgcacta agagcggcgg ttatgatgtt taagcaatac
    26221 ctgcaagtaa cgaaaccagg catcatcttt ggcaacctga tctcggtgat tgggggattc
    26281 ctgctggcct caaagggcag cattgattat cccctgttta tctacacgct ggttggggtg
    26341 tcactggttg tggcgtcggg ttgtgtgttt aacaactaca tcgacaggga tatcgacaga
    26401 aagatggaaa ggacgaagaa tcgggtgctg gttaaaggcc tgatctctcc tgctgtctcg
    26461 ctggtgtacg ccaccttgct gggtattgct ggctttatgc tgctgtggtt tggcgcgaat
    26521 ccgctggcct gctggctggg ggtgatgggc tttgtggttt atgtcggcgt ctatagcctg
    26581 tacatgaaac gccactcagt ctacggcaca ttgattggtt cgctctccgg cgctgcgccg
    26641 ccggtgattg gctactgtgc agtaaccggc gagttcgata gcggcgcagc gatcctactg
    26701 gctatcttca gcctgtggca gatgcctcac tcctatgcca tcgccatttt ccgctttaag
    26761 gattaccagg cggcaaacat tccggtattg ccagtggtaa aaggcatttc ggtggcgaag
    26821 aatcacatca cgctgtatat catcgccttt gccgttgcca cgctgatgct ctctcttggc
    26881 ggttacgctg ggtataaata tctggtggtc gccgcggcgg ttagcgtctg gtggttaggt
    26941 atggctctgc gcggttataa agttgctgat gacagaatct gggcgcgcaa gctgttcggc
    27001 ttctctatca tcgccatcac tgccctctcg gtgatgatgt ccgttgattt tatggtaccg
    27061 gactcgcata cgctgctggc tgctgtgtgg taacaaaacc tctctattaa aaaggtgcta
    27121 cggcaccttt tttattgtct gagatcagag atacggctaa taaccgtatc tcttgacaga
    27181 gtgagcagat gcacataaat taatcatacg gttgatagcc gtattatgta tgagatcgac
    27241 tccctacgat ttcattcagt taatcaaaga agtcataatg cctggaaaaa cagccgctct
    27301 atacgatgtc gataaaacgc tgaaaaatgc acgggtcgaa ctcaaaacta gtcccgacgc
    27361 caaaaacaaa cttcgtgaag ctgcgcaagc cgttggggta gatttaagcg cttttatttt
    27421 gagcgttgct atggaacgcg ccgaaagtgt gcttgataac caacgtcgcc gtgagctttc
    27481 gaatcaaagc tgggaactga tgaaccaact catcgctgaa cctgctcaac cgacgctcgc
    27541 cctcaaggcg ttaatgaaaa ggaaaaacag cgatggtcga caagcatgaa gagattactc
    27601 tgcccatagt cctctcctgt aattatcagt ctgatattac ttatcctggg caaaaacagt
    27661 ttgattgcgg taaccctgtt atcgataaat ttgtacgcgc atcgctaaag aaaagtgtgc
    27721 gtaatagcga ctgtgcggct aaagcactta ttgacagaca aagtggtgaa ctgattggca
    27781 tctgtacttt tacggcatat tcgctggaaa gacaacgcgt ttctggcgtc cttcagggtt
    27841 cacaaccttc agaaattggt gttgtcagat tagtcatgtt ggggggagcg cggaagtatc
    27901 aaaagcgggg ctttggtcag gacctactat gtgatttttt tgaacatgta aaaaaattca
    27961 ccaggcatta ccaattaaag gggtttatct tgatgctaac cctgccgcca ttaactttta
    28021 tgctcgtctc ggctttgttc agctttcagc gacaccaaat gcttttggtg ctgtacctat
    28081 gtttttggcg attcagcata ttctcgcggc ttagattatt atcgccaccc gctagggcaa
    28141 gttttgcctt agccgggggc tgagcagcgt taccactttt gttcaattgc ccccgctttc
    28201 agcaactcca catccttgcc gttaatagtt gccgttgcgg caacgattgt ggtccttaca
    28261 gttgcacaag attttcgctc aaattccggt ttataaagaa taagagacga attactggta
    28321 aatgaaccaa ataggtaatc agtaaagcta atattcagcc ggtttaaacc gaaaggcgcg
    28381 aagccaaccg tttggttaat cttcttaccg gatgggcgac gaatgctgaa tgtcacgcga
    28441 agattggcca cttcgcctaa tttattggtg gtggcgaaga ggctatccat gcaggaaaaa
    28501 cctttatcta aaaaattatc ccgcatccca gaacgggtaa ccgtgatcac gggtaacgta
    28561 tcctcaaccg tttttctgtc aatcatgtct tttcgacaag tagccagaaa gtcttccggg
    28621 ccaccataaa tgtggacacc tttatcaagg tattccttcg ctttcttaca atctacctca
    28681 acatactgac cattggcgta catcatgccc aacctggacc atgctaaatt cataccttgc
    28741 tgcgcggcct gattaaatag tgcatacgct ttggcataat ccacccgcac ctgctcaccg
    28801 tcgtagtaga tactccccag cgctaacgga gcttctctgt caccttgttt agcggctaac
    28861 tcgaaccatt taatcgcttc ctgaacatca ggtttggtat catagcgagg ctgataatat
    28921 tcccagccta acaggctctg cgcgtcgata tctcccgatt cagccatttt atgcagtgta
    28981 tttataacat cgggagcaga tttaccaccg tatatgctgt catacaattc atgcgctttc
    29041 ttacttgcca ccacgcctga tgaaactatc agcattaaag aaaataatat tctattaggc
    29101 aacataaaat atctcttatt tatcttgtac ctgaatatgc tcttctttta ataaatcacg
    29161 ttgttttcca ttcagtatcg ctgtcgcaga tttaatagaa taagtcaaat catgaaaatc
    29221 gcataaatta cgattataca ttgggatgtc tatatagctt tttcttatat gactggaatt
    29281 attatacata tccacaccga gcgtattcaa actaaaagga gggacagaaa gagtatattc
    29341 tttactcact ccctcccccc ccttttagct caagcgtcaa ctgcaaatta gttatactac
    29401 taattttatc tgcattaatt tcaatactga cattgcatat tttgggtttt tcatttgcgt
    29461 taaagccata ggtcccgtaa tgttttaacg tgatgtaagg taaagtatca ccctgtgttt
    29521 ctcgggtttt ctgatcttga ttacaacggg ttaaatattc atccttattg aatttcattg
    29581 aatatttccc cgcacctttt tcgtaataat cccatacctt atcacaatca agcgtttcac
    29641 ctaatccgtt gtaatacatc caacttaacc acaatgcggc tttttttgcc ccttg
//
LOCUS       DBKEKI010000042        29167 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.042, whole genome shotgun sequence.
ACCESSION   DBKEKI010000042 DBKEKI010000000
VERSION     DBKEKI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.042"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(181..858)
                     /gene="narI"
                     /locus_tag="ACUQPX_001816"
     CDS             complement(181..858)
                     /gene="narI"
                     /locus_tag="ACUQPX_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HHV9056544.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(858..1568)
                     /gene="narJ"
                     /locus_tag="ACUQPX_001817"
     CDS             complement(858..1568)
                     /gene="narJ"
                     /locus_tag="ACUQPX_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HHV9056545.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDVVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1565..3103)
                     /gene="narH"
                     /locus_tag="ACUQPX_001818"
     CDS             complement(1565..3103)
                     /gene="narH"
                     /locus_tag="ACUQPX_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HHV9056546.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNTQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3100..6843)
                     /locus_tag="ACUQPX_001819"
     CDS             complement(3100..6843)
                     /locus_tag="ACUQPX_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HHV9056547.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7236..8627)
                     /gene="narK"
                     /locus_tag="ACUQPX_001820"
     CDS             complement(7236..8627)
                     /gene="narK"
                     /locus_tag="ACUQPX_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HHV9056548.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGESFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            8966..10762
                     /gene="narX"
                     /locus_tag="ACUQPX_001821"
     CDS             8966..10762
                     /gene="narX"
                     /locus_tag="ACUQPX_001821"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HHV9056549.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10755..11405
                     /gene="narL"
                     /locus_tag="ACUQPX_001822"
     CDS             10755..11405
                     /gene="narL"
                     /locus_tag="ACUQPX_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HHV9056550.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="ACUQPX_001823"
                     /gene_synonym="iatA"
     CDS             complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="ACUQPX_001823"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HHV9056551.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            12985..13338
                     /gene="ychN"
                     /locus_tag="ACUQPX_001824"
     CDS             12985..13338
                     /gene="ychN"
                     /locus_tag="ACUQPX_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HHV9056552.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="ACUQPX_001825"
     CDS             complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="ACUQPX_001825"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HHV9056553.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="ACUQPX_001826"
     CDS             complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="ACUQPX_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HHV9056554.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14735..15835
                     /gene="chaA"
                     /locus_tag="ACUQPX_001827"
     CDS             14735..15835
                     /gene="chaA"
                     /locus_tag="ACUQPX_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HHV9056555.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16240..16347
                     /locus_tag="ACUQPX_001828"
     CDS             16240..16347
                     /locus_tag="ACUQPX_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HHV9056556.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="ACUQPX_001829"
     CDS             complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="ACUQPX_001829"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HHV9056557.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(17386..18187)
                     /gene="sirB1"
                     /locus_tag="ACUQPX_001830"
                     /pseudo
     CDS             complement(17386..18187)
                     /gene="sirB1"
                     /locus_tag="ACUQPX_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
     gene            complement(18191..18583)
                     /gene="sirB2"
                     /locus_tag="ACUQPX_001831"
     CDS             complement(18191..18583)
                     /gene="sirB2"
                     /locus_tag="ACUQPX_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HHV9056558.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(18580..19413)
                     /gene="prmC"
                     /locus_tag="ACUQPX_001832"
     CDS             complement(18580..19413)
                     /gene="prmC"
                     /locus_tag="ACUQPX_001832"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HHV9056559.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(19413..20495)
                     /gene="prfA"
                     /locus_tag="ACUQPX_001833"
     CDS             complement(19413..20495)
                     /gene="prfA"
                     /locus_tag="ACUQPX_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HHV9056560.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(20537..21793)
                     /gene="hemA"
                     /locus_tag="ACUQPX_001834"
     CDS             complement(20537..21793)
                     /gene="hemA"
                     /locus_tag="ACUQPX_001834"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HHV9056561.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            22007..22630
                     /gene="lolB"
                     /locus_tag="ACUQPX_001835"
     CDS             22007..22630
                     /gene="lolB"
                     /locus_tag="ACUQPX_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HHV9056562.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVITPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            22630..23481
                     /gene="ispE"
                     /locus_tag="ACUQPX_001836"
     CDS             22630..23481
                     /gene="ispE"
                     /locus_tag="ACUQPX_001836"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HHV9056563.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            23632..24579
                     /gene="prs"
                     /locus_tag="ACUQPX_001837"
     CDS             23632..24579
                     /gene="prs"
                     /locus_tag="ACUQPX_001837"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HHV9056564.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            24704..26383
                     /gene="dauA"
                     /locus_tag="ACUQPX_001838"
     CDS             24704..26383
                     /gene="dauA"
                     /locus_tag="ACUQPX_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HHV9056565.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSTLVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(26438..26716)
                     /gene="ychH"
                     /locus_tag="ACUQPX_001839"
     CDS             complement(26438..26716)
                     /gene="ychH"
                     /locus_tag="ACUQPX_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HHV9056566.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            26994..27578
                     /gene="pth"
                     /locus_tag="ACUQPX_001840"
     CDS             26994..27578
                     /gene="pth"
                     /locus_tag="ACUQPX_001840"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HHV9056567.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            27695..28786
                     /gene="ychF"
                     /locus_tag="ACUQPX_001841"
     CDS             27695..28786
                     /gene="ychF"
                     /locus_tag="ACUQPX_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HHV9056568.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         7006 a         7693 c         7327 g         7141 t
ORIGIN      
        1 cttccttgaa taatcaaaac ctgttactcc gagctaattt tgcccgatgg caccacgttt
       61 accgggccta caatatcctc ccctgtaggt cggataagcg aagcgcatcc gacaactcac
      121 cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac taaaattcgt
      181 ttagtgacga gcgcgcacca gctggtactt acgtgtcaga tactccaccg gtacgctcca
      241 gatgtgtacc agacgcgaga acgggaacag caggaataac gtcatcccca gcaccaggtg
      301 cagacggaag ataaacgcca caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac
      361 caccgactgc gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcgga
      421 gaacggaatg gtcagcaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat
      481 atccgctccg gtagtggttg cacgcacgcg tgggctgaac atacgacgtt tcagcaaaag
      541 cacgccgcca atcagacaca gcacgccgct ggcaccacca gcaaacatcg ccattttctg
      601 tttcacttca atcggcagcc aggcttcata catccagtgc ggcgtcaaca taccgaagaa
      661 gtgaccgaca aaaatcccca gaataccgat atggaacagg ttcgacgcca ggttcatccc
      721 tttgcgatcc agcatctggc tggacgccgc gcgccaggtg tactgcccgt agtcataacg
      781 cagccagcta ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa
      841 catattcagg aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg
      901 acggcaccgg caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca
      961 gcaaagaatt taacctgctc ttcttcccag acaacatcca gcgcctgcgg cgtatcatcg
     1021 cgcgcttcgt cggcaatttt ttccgccact ttgtcgctgt cgatagcggt attggccaat
     1081 ttcagcagca gatcaaacat cacggcataa cggctttcac gctgttgcag acgcgcgctc
     1141 agcaatgcca gaatcggcgc gatatctttc aaaccttcca cggcttcgct ttgcggcagc
     1201 tgcgacaggt actccagata cagcggcaga tggtccggta attcgcggct gtttaactgc
     1261 aagccgtgct gctcgtactg cgccagcagg tccaccatcg cctggccgcg gtcacgggat
     1321 tcaccgtgaa catgttcaaa tagcaacagt gacgtggcgc ggccacggtc gaacagttcg
     1381 ctgtactggg cctgggcatc gagcggatcc atcgccgtta aatcgcgcag gaaaatgccc
     1441 agcgcatggg catcttcttt cgacagattt ttcgacgcag caatcgcctc aaacatctct
     1501 tgttgatgct gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt
     1561 tcgatcatgg atgcggctcc gttttgctgg tcacatcgat ggcatcgata cgacggctgt
     1621 tgaacagatt gaatttggta tctgagccgt ggcagccatc accaaaggta aagccgcagc
     1681 catttttctc cgggaaggct tcccgtgcca gttcacgatg actactcggc accacaaagc
     1741 gatcttcgta gttagcaatc gccagataac ggtacatctc ctgtgcctgg gcttcggtca
     1801 gaccgacctc ttccagcgca cgggtatcaa ctttaccgtc aacagtttca gcacgtttgt
     1861 aatgacgcat cgccagcata cgtttcagcg cacgcagtac cggtttggta tcaccggcgg
     1921 tcagcagatt cgccagatac tgtaccggaa tacgcaagct ttcgacgtct ggcagaattc
     1981 cgttactgcc cagctcgccc gcgtctgctg cagactgaat cggagacaga ggcggcacgt
     2041 accagaccat cggcagtgtg cgatattctg gatgcagcgg cagcgccagt ttccattcca
     2101 tcgccatttt ataaaccggc gactgctgtg cagcttcaat aacgctcagc ggaataccgt
     2161 ctttaatcgc ctgctcgatg actttcggat cgttcggatc gaggaacacg tccagctgac
     2221 gctggtagag atctttctcg ttctcggtgc tggctgcacg ttcaatcgcg tcggcatcgt
     2281 acagcagcac gccaagataa cggatacgac cgacacaggt ttctgagcac acggtcggct
     2341 gacccgcttc aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt
     2401 tgaagtagat ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat
     2461 cctggtcgat caggacgatg ccatcttctt cacgcttgta aatcgcaccg ctcgggcagg
     2521 tcgccacaca tgccgggttc aggcagtgtt cgcacaggcg cggcaaatac atcataaagg
     2581 tgttttcgaa ctggctatac atcgccttct gggtgttgtc gaagttcttg tctttcgcca
     2641 gtttatcaaa ctcaccgccc aggtcatctt cccagttcgg ccctttttcg attttcgcca
     2701 tccgttcccc ggtaatcagc gaacgcggac gggcaatcgg ctgcgatttg ctgccttccg
     2761 gcgcggtatg caggttctga taatcaaaat cgaacggctc gtaataatcg tcgatccccg
     2821 gcagatgcgg gttagcaaag attttaccca gcagcatggc tcggttaccc atgcgcggtt
     2881 gcagtttgcc attgatttta cggatccagc cgcctttgta tttttcctgg ttttcccagt
     2941 cagtcgggaa gccctggccc ggcttggttt ccacgttgtt gaaccacgcg tattccacgc
     3001 cttcacgact ggtccagacg tttttacagg taactgaaca ggtgtggcag ccgatgcact
     3061 tatcgagatt cagcaccatg ccgacttgtg aacgaatttt cattttacgc tctcctgtac
     3121 ctggtcattg ccttcgccat ctaaccagtc aatgttcttc atcttacgca ctacaacaaa
     3181 ctcatcgcgg ttagaaccta cggtgccgta gtagttaaag ccgtatgcca gatgggcata
     3241 gccgccgatc atatgcgtcg gtttcggcgt gatacgggtg accgagttat ggataccacc
     3301 acgctgttgg gtaatttccg aaccaggcag gttaacgata cgttcctgcg cgtggtacat
     3361 catggtcatc cctgccggaa cacgctggct gacaaccgca cgggcagtca gtgcaccgtt
     3421 gctgttgaag acttcaatcc agtcgttatc ggcgataccc agatctttgg catcggcttc
     3481 actcaaccag accaccggac caccgcgacc taaagtcagc atcagcaggt tgtcgctgta
     3541 ggtggagtgg ataccccact tctggtgcgg cgtcaggaag ttgagcgctt tttccgggtt
     3601 gccgttggat ttctggccta tcacttcttt caccgaacgg gtgtcgatcg gcggacgata
     3661 aaccagcagg ctttcaccga aatcacgcat ccactggtga tcctgataca gttgctgacg
     3721 accagagagc gtacgccatg ggatcagctc gtgaacgttg gtgtaaccgg cgttgtaaga
     3781 aacgtgttca tcttccagac cagaccaggt cgggctggag ataattttgc gcggctgtgc
     3841 ctgaatatcg cggaagcgga tcttctcgtc ttctttattc agcgccagat gcgtatggtc
     3901 acgaccggta aattcgctca gggcagccca ggctttcacg gctacctgac cgttggtttc
     3961 cggtgccagc gtcaggatca tctctgccgc atcaattgcg gtgttcagca tcggctggcc
     4021 ttttgccgga ccttccgctt tggtgtagtt gagcttacgc agcagatcca tctcgctctg
     4081 ggtgttccag gcaatccctt taccgccgtt accgattttc tccatcagcg ggccgataga
     4141 ggtaaagcgt tcgtaagttg ccggataatc gcgctctacg accataatgt gcggtgcagt
     4201 tttgcctggg atcaggtcgc attcgccttt tttccagtct ttcacatcca gcggctgcgc
     4261 cagttcagcg gcagagtcgt gctggatagg cagcgtgacg atgtcggttt ctttacccag
     4321 atggccgacg cacacttcgg agaatttctt cgcgatggct ttgtagattt cccagtcgct
     4381 tttcgcttcc caggccggat cgaccgccgc agacagcggg tgaataaacg gatgcatatc
     4441 cgaagtattc atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga tgtcggaata
     4501 gagacaggtg ctcgacagac ggaagtccag cgtaaccacc agatccagct tgccttccag
     4561 accattgtcc tgccagtcca cttcttccgg cttcacgccg ccctgttgcc ccagatcttt
     4621 accctggata ccgtgctccg tccccagcag gtacttgagc ataaactcat gacctttacc
     4681 ggaagaaccg agcaggttag aacgccagat gaacaggttg cgcgggtggt ttttaccgtt
     4741 ttctggttgt tctgccgcaa aacggatgga accctctttc agggatttca ccgtatagtc
     4801 caccggattc atcccggctt tttccgcttc gccagcgata gtcagcgggt tagtgcctaa
     4861 ctgcggtgca gacggcagcc agcccatgcg ttccgcacgg acgttaaagt cgatcaagtg
     4921 tccggtatag cgggatttgt ccgccatcgg tgacagcaac tcttccgccg tgacggtttc
     4981 ataacgccac tggctggagt ggttatagaa ataagaagtg ctgttcatgt gacgcgccgg
     5041 acgctgccag tcaagggcaa acgccagcgg ctgccagccg gtttgcggac gcagtttttc
     5101 ctgacctaca tagtgcgccc agccgccccc gctctgaccg acacagccgc agaaaatcag
     5161 catgttgatc agaccacgat agttcatatc gaggtgatac cagtggttca gccccgcacc
     5221 gacgataatc atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt cacgggcgat
     5281 gcgaataatc tggctgcgag aaacgccggt aatctgctcg gcccaggccg gggtataagc
     5341 tttcacatcg tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt ccagaccgta
     5401 gtttgccagc gtcagatcat aaacggtggt caccagggcg gtgctgccat cagccagttg
     5461 caggcgtttc accggcagtt tgtgcagcag cacgttttcc agttccactt tgttgaagtg
     5521 ttccgtgccg tcgccaccaa agtacgggaa gcctacctca gcgatctcat cctggctacc
     5581 cagcaggctc agttgcagct cggtttcttc gccagttttg ccgtcgcgct gctcaagatt
     5641 ccatttgccc ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa ccatttcgcc
     5701 attggtatta aaggcgacag ttttccattc cggattgttt tcctggccca gcgcatcaac
     5761 cagatcagca gcgcgcagca tacgacctgc agcgtagtaa ccgtcgcgtt cttccagcat
     5821 caccagcatc ggcatgtcgg tgtagcgacg cacatagtcg gtgaaatact ggcttgggtt
     5881 gtcgaggtgg aattcacgca gcattacgtg gcccatcgcc agcgccattg ccgcatcggt
     5941 gccctgtttc ggtgccagcc acagatcgca cagtttggcg atttcagcgt agtctggtgt
     6001 gacggcaaca gttttggtgc ctttgtaacg cacttcagta aagaagtgag catccggggt
     6061 acgcgtctgc ggcacgtttg aaccccaggc gatgatgtag ctggagttat accagtcagc
     6121 agattccggt acgtcagttt gctcgcccca ggtttgcgga gacgcaggag gcaggtcgca
     6181 gtaccagtcg tagaagctta agcaagtacc gccaatcagc gagagatagc gtgcacccga
     6241 tgcgtaagaa accatcgaca ttgccggaat tggcgagaaa ccagcaacac gatccgggcc
     6301 gtagtttttg atggtgtaaa cgttagatgc ggcgatcagt tcgttcacct cctgccaaga
     6361 agaacgaaca aatccaccgc gtccacgcgc ctgcttaaag cttttcgctt tatcggcgtc
     6421 ttcaatgata gaagcccatg cctcaaccgg atcgctatgc agcgccttcg cttcacgcca
     6481 cattttcatc aggcgtttgc gcatcatcgg gtatttcagg cggttggcac tgtaaagata
     6541 ccaggagtag ctggcaccgc gcgggcagcc gcgaggttca tggtttggca gatccggacg
     6601 ggtacgcgga tagtcagtct gctgggtttc ccaggtgacc agaccgtttt tgacgtagat
     6661 tttccagctg caggagccgg tgcagtttac cccgtgggta gagcggacga ttttgtcatg
     6721 ctgccaacgc tggcgatatc catcctccca gtcacggttg gtattgagaa gctggccatg
     6781 cccatcggca aaggtttcac ccttctgttt gaagtagcga aaccggtcca ggaatttact
     6841 catcggtttt ctcctgtggg agcctgtcgg ccctctgata aatcgacatt gctaatgttt
     6901 aagggcaagg taacgctctg aaacagcgtg ggaattgata acgatcaaga gtgatgggga
     6961 tgaaaaataa agtaaattcc taagataccc ctttaggata attaacagtt acagcataac
     7021 atgctgtaaa ttaagatgtt ttatacctgt agttatttaa actattaaga tttttatgca
     7081 aatgggtatt aaggagtatt ccccatgggt aacatattaa tggctcttaa tgaaatccgg
     7141 cgaaataagc catataacac taatatgttg taactaaatg acgcataaaa aaagcgcggc
     7201 atcatgccgc gcaaaggata atcaaatatt aagatttatt ttttagaatg ccgaccatat
     7261 accgcccagg taatcactac gcaggcgata tagaaaatca aaaatacttt cattgcacct
     7321 actggcgaac ccgttaatgc cagcgagcta ccaaacgctt tcgggataaa gaagccacca
     7381 atcgcgccaa tcgcagagat gaaacccagc gccgctgccg tgtcggttgc cgcttcacgc
     7441 atcgcacgtt cgtcagaacc cccttctgct ttcacgcgat ccattgtcag tttacggaag
     7501 atcactgaaa tcatctggaa agtggaacca ctacccagcc cagctgtcag gaacagcgcc
     7561 aggaagaccg cgaagaacgc catgaagctt tcgccctgcc cgtcagtcgg taaggtcagg
     7621 aacagcaggc cgctgaaaat cgccatcaga ataaagttca ccagcgtgac acgagttccg
     7681 cccagacggt cagataatgc accacctgca gaacgcgcca gcgcaccaat aaacggcccg
     7741 aagaaagcgt attgcagaat ctgaacatcc gggaactgcg tttttgacag catcgcaaag
     7801 cccgcggaga agccgatgaa ggagccgaag gttgccagat acagcaggct cataatccac
     7861 agatgacccc gtttgagtac cggcaactgc tccttgatgg aggctttcga ggtagcaaga
     7921 tcgttcatgc caaaccacgc cgcaatggtg aagatggcaa ggaacggcac ccatatccag
     7981 gacgcattcg ccagatacag ctcagtccca tccggctgtt tgacgccctg gctaccaaat
     8041 actgcgaaaa tcgacagtga taccaccagc ggagcaacca actgcatgac gctgacgccc
     8101 atgtttccca gaccaccatt cagacccagc gcgccaccct gcttctgttt cggaaagaag
     8161 aagctgatgt ttgccatact ggatgcgaag ttcgcgccag caaagccgca tagcagagag
     8221 atgatgatga agacgctata aggcgtggag gtatcctgca cggcaaaacc cagccagacg
     8281 caaggaataa tcagaatacc ggtgctgaac gccgtccagc gacgaccacc gaagatagga
     8341 accataaagg agtatggaac acgtaataac gcgccagaaa ccgaaggcag cgcagtcaac
     8401 ataaatagct gatcggtcgt aaaattaaaa ccgactttcg gtaagttcac cgcaacagcg
     8461 ctgaacaaca tccatacgca aaacgccagc agcagacagg gaacggaaat ccacaggttg
     8521 cggctggcaa tacgttgacc gcgttgttgc cagaacgcag gatcttccgg tcgccaatct
     8581 gttatgacag ctccagtagc cctttcggag gcagatgagt gactcataga cacctctgat
     8641 actcgtttcg atgtctgcca ccttagtgtc tggagctaaa ggcaatttga tgtaaatcaa
     8701 acgataagat aactttatct atcattgata tttatcatta cccatagtga gtacagtgat
     8761 ttcataaaaa ttatgagatt tttcacggtg ctgtagaatc cctaccctta ccgatgtaaa
     8821 gcgactaacc acacggcaaa taaggagtaa ctctttccgg gtatgggtat acttcagcca
     8881 atagccgaga atactgccat tccagaatat atcgtcacat tcattaaggt tattgctcat
     8941 ttaaagcctg aaggaagggg tttacatgct taaacgttgt ctctctccgc tcaccttggt
     9001 taatcaggtt gcgcttattg tgttgctttc tactgctatt ggactggcag ggatggcggt
     9061 ttctggctgg ctggtgcaag gcgttcaggg cagcgcccat gcgatcaaca aagcgggatc
     9121 gctgcgcatg caaagttacc gtctgttggc ggcagtgcca ttaagcgaga aagacaagcc
     9181 cttaattaaa gagatggaac aaacggcatt tagcgccgag ttgactcgag cagcagaacg
     9241 agacggacaa ctggcgcaat tacagggttt acaagattac tggcgtaatg aactgatccc
     9301 tgcgctgatg cgtgcacaaa accgagaaac ggtgtcagcg gatgtcagcc agtttgttgc
     9361 cgggcttgat cagctggtat ctggttttga ccgcaccacg gaaatgcgta ttgagacagt
     9421 ggtactggtc catcgggtaa tggcggtatt tatggcactt ttactggtgt tcactattat
     9481 ctggttgcgg gcgcgactgc tacaaccgtg gcggcaactg ctggcaatgg cgagtgccgt
     9541 cagccatcgc gattttaccc aacgcgcaaa catcagcggg cgcaacgaaa tggcgatgct
     9601 tggaactgcg ttgaacaata tgtctgcaga actggccgaa agttatgccg tacttgagca
     9661 gcgggttcag gagaaaaccg ccgggctgga gcataaaaat cagatcctct cttttttatg
     9721 gcaggctaac cgccgtttgc attcccgcgc cccgctgtgt gaacgcctgt caccggtact
     9781 caacggctta cagaatttaa ccctgctacg tgatatcgaa ctgcgggtgt atgacactga
     9841 tgatgaagag aatcatcagg agtttacctg ccagccagat atgacttgtg atgataaagg
     9901 ctgccagctc tgcccgcgcg gcgtattacc cgttggtgat cgcggcacga ccctgaagtg
     9961 gcggctggct gactctcata cgcagtacgg tattttgctg gcgaccctgc cgcaggggcg
    10021 tcatcttagc catgatcaac aacaactggt ggataccctg gttgaacaac ttaccgccac
    10081 gctggcgctg gatcgccatc aggaacgtca gcaacagttg atcgtgatgg aagagcgtgc
    10141 caccattgcg cgcgaactgc atgattctat tgcccaatct ctctcttgca tgaagatgca
    10201 ggtgagttgt ttacagatgc agggcgatgc gctgccagaa agcagccgcg aactgttaag
    10261 tcagatccgt aacgaactga atgcatcctg ggcgcagttg cgtgaattgc tcaccacatt
    10321 ccgcttgcag ctcaccgagc ctggattacg tccggcgctg gaagcgagtt gcgaagagta
    10381 cagcgccaaa tttggcttcc cggtgaagct ggattatcaa ttgccgcctc gtctggtgcc
    10441 ttcacatcag gcaatccact tgttgcaaat tgcccgtgag gcattaagta acgccctcaa
    10501 acattcgcaa gcgagtgagg tcgtggtgac ggtggcgcaa aacgataatc aggtcaaact
    10561 gaccgtccag gataacggct gcggcgtgcc tgaaaatgcc atccgcagca atcactacgg
    10621 catgataata atgcgcgacc gtgcgcaaag tttacgaggc gattgccgcg tccgccgtcg
    10681 tgaatcaggt ggcaccgaag tggtggtcac ctttattccc gaaaaaactt tcacagacgt
    10741 ccaaggagat acccatgagt aatcaggaac cggctactat cctgctgatt gacgatcacc
    10801 cgatgctgcg aactggcgta aaacagctta tcagtatggc accagatatc accgtggttg
    10861 gcgaagcgag taatggcgaa cagggtattg aactggcgga atctcttgat cccgatctga
    10921 tcctgttaga tctcaatatg cccggcatga acggtctgga aacgctggat aaactgcgcg
    10981 aaaagtccct ctcagggcgc attgtggtat tcagcgtctc taaccatgaa gaagatgtgg
    11041 tcaccgcact gaaacgcggc gcggatggct atctgttaaa agatatggaa ccggaagatc
    11101 tgctgaaagc attgcatcag gcagctgctg gcgaaatggt attaagcgaa gcattaacgc
    11161 ctgttctggc cgccagcttg cgcgctaacc gtgccactac tgagcgcgat gttaaccagt
    11221 taaccccacg cgagcgcgat attctcaagc tgattgccca gggtttgccg aacaagatga
    11281 ttgcccgccg cctggatatc accgaaagca cagtaaaagt gcacgtcaag cacatgctga
    11341 agaaaatgaa gctcaagtct cgcgtggaag cagcggtatg ggtgcatcag gagcgcattt
    11401 tctgattacg gttcccagcg cagttcgtcg tttgacaatg catcgaacgg ttcgacaagc
    11461 gttagcgtga tctcattgga ggagacacgc tgcccctggt tatcttccac caccacagac
    11521 aatcgccagt gattgcttgc cccttccccg ttctgccagt caggcatgat cagcgtccag
    11581 ccctccgcgc tgttggcttg tgcgcccggc gtcaaactta atatctgcgt atcgccctgc
    11641 caaatcagtt gccgaatacc gtaacgactg cggatttgta atttcagcgg cactgtttcg
    11701 ccaggtttta gatcccacgg cggtgtcgcc agaaacaccg ttaacgtttt tcgctgtcgg
    11761 tactcaagag tcggtagatt atttcgctgc ggattgtcat agcgactacc acgtaacgac
    11821 tgactttcgg caacctcgcc cgcagaaagt tgttttttga gcggtacacc aaagcggtaa
    11881 ttaagattca gcccgaggtt attttgattt tcgccacttt ccccctgttt atgctgggcc
    11941 gtcacagtaa ctaatggcac aggggtgtaa tttaatccca gactcaacgc gacgggattg
    12001 tgataacccg taccagagtt aaacaaatca acacgatcac caaaatactg ttctaggctg
    12061 acactggtat tgaggtgttg atagaacggc atgcgcatcc gagctgtcag gtcgtacccg
    12121 cgcgccatcc gttgttcctg cgtggctgtc tgttcatgcc atgcagcaaa cggctgataa
    12181 aagtttgccg ataatcgcaa atattcgccc cacgcttcgg caccaaagcc cgctcgctga
    12241 agattttcgt ccagcaagtt gtcataaaaa gtgttataac ccaccagcca gttgccgcgc
    12301 gcccagcgtt gcccaacgcc cacattgctc accaacccat tatcctgctg agtaagacca
    12361 agctggctcc aggtgagata acgatcatta tcttgtaacg gcacaaacca gcttccacga
    12421 ctgccggtga aatgtccttc gttatccact ttgacgtcaa cactggcatt tccccacggt
    12481 gatagccagg actctacgtg ctgattaacc tgttgactaa gcgcgtcgcg gacttttccc
    12541 aatgcgaaag cttttgcctg ctcgccagta tccagcccgt tatcattcat actggtttcg
    12601 ccgaaatctt tcacaatttc agcaaagtgt ttttccccat catgattttc gggagccatg
    12661 cctaaatccg gcagaccatc attgttatta tcaaagggat ttgccgcttt ttgctcgaag
    12721 gtagattgtg cgttagctgt accgcctgct acaagcaaga gtaaataaaa cgggataatg
    12781 cgaggaacga aacggctcaa aacgtcggta acttccggta ataatgaaaa accatagcct
    12841 taaagaataa cgattactgg caacttttgt cgtattttct cgcgctttca ggaaaatcct
    12901 taaaaatgtg accgattggt cagacgctgc agaagcagta agatagaaga tgagtgtcta
    12961 attccctgaa tacaggagta aataatgcaa aaaatcgtga tcgttgccaa tggcgcacct
    13021 tacgggagcg aatccttgtt taacagcttg cggctggcca ttgcgttacg agagcaggag
    13081 agcaatctgg atctgcgtct gttcctgatg tctgatgcgg tcacagccgg gttgcgcggg
    13141 caaaaaccag gggaaggcta caacattcag caaatgctgg agatccttac cgctcagaat
    13201 gtaccggtga aattgtgcaa aacctgtacc gacgggcgcg ggattagcac acttcctctg
    13261 attgatgggg tggaaatcgg tactcttgtg gaactggcgc aatggacgct gtcagccgat
    13321 aaagtgctca cattttaatg tttaaggggg cgtctgacgc cccactatga gggaagttta
    13381 ctcaggcgaa tcccggcctt aacacaccat ccggataatt ctccgccagt agttttttta
    13441 ccgataccag caaatcattc agcccatcgt cctgcattcc cagtttgata agctcctgtt
    13501 ccagtgaaaa caggtattgt gcattggttc ccagcggacc gctcgccgcc gcaatcaacg
    13561 gcgcgatgac ctgagcgcga gtgtcagatt catattctgg atgtcgcggg tccataataa
    13621 acacaatggc gtttactgtg cgtccatcat caagatcaag ctgacaccag gttggcagat
    13681 aacagccggt aatcatctcg cgcttccaca acagggttag ttcctgctcc agcgtctctt
    13741 ctggtagtcg ataggcgacg cctgtggtgc gtccgccctc tttcagtgca agcatccgtc
    13801 ccggctggtg cgcagtcccg cgcccggcgg tcaggcgcag gcaaaacgcg cgatgccatc
    13861 caaccagtgt accggtgcat gattcggtaa actccagtgc cggattccac atcaacgatc
    13921 cataaccgaa gatccacacc ggtccctcat caggtcgaca agccagcgtc gccgccagcg
    13981 aagccgcccg ttgttctgct gaccataaga gtgattcttc aatggcacca aatgccgttt
    14041 tacaatcggc attcatcaag aaatcacgcg ttatcacctt acacctccgc cactgcatac
    14101 ttccctgcga caactttttg gtgtcttccg gacattttta ccgctcattt tgaaaccact
    14161 aacgggacaa tatgcaatcc acggaccact tgcaaggcaa caccgcacga atagctttaa
    14221 ctaagctacc ggttttacga ttttttatgc catttatcat catccccttt ggcatattca
    14281 tgcttcacag cagcccaggc cactttatgc gcggtttctt cgcgactggc gtcatcacgc
    14341 cgatcttctt tatctttata ttgatcccat gcgctgttga acgcttcttt atagatatcc
    14401 tgggcatgag acggtagaac gtgctttacg ctttccggca gatcgctttt cgttttatac
    14461 ggcatcgtta actcctttta caaagttgaa cattaagtgt ggtcaacaat atctactctt
    14521 gccaaaacat aagaattttc atgtcagact gttcttaaga aacgctaaaa caaacattgt
    14581 ctaaacgtaa tacttatttt tatgccccaa tgagtaaaaa atcgacagcg gggtaaaaat
    14641 tcgtaaaatg ttaccgatat ccagacactt tctgttattt ttcctaccta ttttttacat
    14701 tttcgataaa ttcatctgca aagggataac cataatgtca aatgctcaag aggcggtaaa
    14761 aacccgccac aaggagactt cgcttatttt cccggttctg gcgctggtag tgctgttcct
    14821 gtggggaagt agccagacac taccagtggt cattgccatc aatcttcttg cgcttattgg
    14881 tattttaagt agcgccttta gtgttgtccg tcatgcggac gtattagccc atcgcctggg
    14941 agaaccttac ggttcgctta ttcttagcct ttcagtggtt attcttgaag tcagtttgat
    15001 ttcagcttta atggcaaccg gcgacgccgc gccaacgcta atgcgtgata cgctctattc
    15061 aatcattatg attgttaccg gtgggctggt tggcttttca ttattgttgg gcggtcgtaa
    15121 gtttgccacc caatatatga atctgtttgg tatcaagcag tatttaattg cgctgttccc
    15181 cctggcgata atcgtactgg tatttccaat ggctctgcct gcggcgaatt tttcaaccgg
    15241 tcaggcgtta ctggtagcat taatttctgc ggcaatgtat ggcgtatttt tgctgatcca
    15301 gaccaaaacg catcaaagtt tgtttgtcta cgagcacgaa gatgacagtg atgacgacga
    15361 cccgcaccac ggtaaaccgt ctgcccatag cagcctgtgg catgctatct ggttgattat
    15421 ccatctgatt gccgttattg cagtcaccaa aatgaacgcc agctcgctgg agacattgct
    15481 cgacagcatg aatgcccctg ttgcctttac tggcttcctg gtggcactgt tgattctgtc
    15541 gccggaaggt ttaggtgcgt taaaagcagt gttgaacaac caggttcagc gcgcgatgaa
    15601 tctgttcttt ggttcagtgt tagcaacaat ttcgcttacc gtacctgtcg tcacgctaat
    15661 tgcctttatg acgggtaacg aattgcagtt tgcacttggt gcgccagaaa tggtggtgat
    15721 ggtggcctct ttagtgctgt gccatatctc cttctccacc ggacgtacta acgtgctcaa
    15781 tggcgcagcg catctggcac tgtttgccgc ctatttgatg acgatatttg cctgacagtt
    15841 atatctcctc cgatgacgga aatcgggaga gataagataa attcctgctt attgtatttc
    15901 ttcatactac ctggacagct cgttaatcag aattgcgagc ctgtaccaca acctgcacga
    15961 aaccactgca acagctgttg aaaagccgga atgtactctt tattgcaaac atttcaggga
    16021 tgaagtgaag atgaattgag acgtagtatc cacaccaagc cggaattatc cggtgaggcg
    16081 caatgttgcg ggggctttat ccctggtggc attggttgct ggaaagagaa aacccccgca
    16141 cgttgagagg taaaacctga caacaaaacg ggggctaatc ttgaaaccag acacttcaag
    16201 aatagccgca aaatgttgtc attacaacaa ggcggctata tgacgctcgc gcagtttgcc
    16261 atgattttct ggcacgacct ggcagcaccg atcctggcgg gaattattac cgcagcgatt
    16321 gtcggctggt ggcgtaaccg gaagtaaggt gaaaacgggc gtcaggctgc cgtgatggca
    16381 atatgcgccc gaaaccggcc gcaggggtaa cagcctgcgg ccttttttgt atctggcacc
    16441 catgaaaaaa gtcttaacgc agaacgctaa gactttattt ttcaagcaaa aaagattact
    16501 tgctggtatc cagttcttcg aaacctttca ccagatcatc aatcgctttc atctgcttga
    16561 ggaacggttc cagtttagcc agcggcagcg cggatggacc atcacatttc gcatgttccg
    16621 gatccggatg cgcttcaata aacagccccg ccagacctac cgccataccg gctcgtgcca
    16681 gctcagccac ctgagcacga cgaccaccgg aagctgcgcc aaacggatcg cggcattgca
    16741 gtgcgtgggt cacgtcgaaa atcaccggcg agttaccaga cactttcttc ataatgctga
    16801 agcccagcat atcgacaacc aggttgtcat agccgaagtt agcaccacga tcgcaaagaa
    16861 tcactttttc gttgccgcct tctttgaatt tatcaacgat attacccatc tgtcccgggc
    16921 tgacaaactg tggtttcttg acgttaatta ccgcaccggt tttcgccatg gcttcaacca
    16981 ggtcagtctg gcgagcaaga aacgccggca actgaatcac atccacgaca tcagcaacgg
    17041 gctgtgcctg acttggttcg tgaacgtcgg tgataatttt cacgccaaaa gtctgcttca
    17101 actcctggaa gattttcatc ccttcttcca ggcccggtcc acgataagag tggatggagg
    17161 aacggttggc tttgtcaaaa gaggctttga acacgtaagg gatacccagt ttctgggtca
    17221 cagttacgta gtgctcgcaa atgcgcatcg ccagatcgcg agattccagc acgttcatac
    17281 cgccaaacag tacgaacggc aggtcatttg ctacgttgat gtcgccaatg ctaaccactt
    17341 tttgtttcat aggatcgcct taatcttgag taaaatgtcg attaattaat gcagcacaat
    17401 atgtttatgc gcgatgttat ttatttgcgc acggatcatt tcgctgatcg ggtcttccgg
    17461 acactgttca acgaaataac ttaaatcgtt caacgcaacg tgttcgcaat ccagttgcgc
    17521 ataaatcaac ccgcgatcgc gaatttcata gggatcttca gtgtaataaa gcttcgctgg
    17581 tgcgtaacgc cagctccatc tgattttctt ccatcaacga ggctttgagt gtatccagca
    17641 atttgcggat tacctcaatg ttatcagctt catcaaggtc ttcataaaac agttccgccg
    17701 acgggctgat atttcccttt aaccatacgt ccagcatatg ttcgcttaac gattcaccgt
    17761 taaaaggatt aatcagccaa atttcgccat ccggacattc aatgcgcaat atcagctgcg
    17821 tagggaaaat caccggcagc agcggcaaat cgagacgatt cgcgacccat aataaaaccg
    17881 cacctaatga taccgcactg ccctgtcgat tctttaacac ctggtccagc cataatgcat
    17941 cggaaagacg ataaacgccg cgtgaggctt taaatcccca gtcgccgtaa aacagcgcga
    18001 tcagtttttc cagctgctct tctaaaggca gaagctggct gatttcttcc ttcgccagac
    18061 taacgagacg ctccagttcg tcgtaaacat cttgcgaggg aaaatcgcgg cggattgctt
    18121 cgcaagccag gatcatgcct tcgcacaatg gcgctttatt aaattcgaaa tcagctaacg
    18181 atctcatgac ttaccccagt aacggtactt ttgtggtggc gagtttaatg atgatgtaca
    18241 gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg ctgactgtgc atacgacgat
    18301 aatcgagtgc aataaaaccc aaaacgatat aaatgataac tccaaacagc ttttcagtca
    18361 gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt agccatcaac gcaatgccgc
    18421 ttaacaataa caccgtatcg acaaccggcg gcacaatgcg cgtccagcga gcaaatgcct
    18481 gtggatgctt ctgataacgt agccagaaac gtaaggttaa tagcccaaca gaaagcgcga
    18541 tactaataag atgaacacta agcagtgtag aaaaacttgt cattgataat agcggccgag
    18601 cgttacgcgc tcgttatcac catagtcacg gcaggtttcg acgtcatgat accccgcgag
    18661 gataaatgct tgtcgcaccg cttcgccctg ctgccagcca tgttccagaa gcagaaagcc
    18721 gccggatacc agcgcgttac gcgactgttc gatgatatgc acgatgtctg ccattccact
    18781 gtctgccgca accagcgcag tgagcggctc aaagcggaca tcgccttgtt gaagatgtgg
    18841 gtcctgctcg tcaatatacg gcggattgct gacaatcatc gcaaactgct gcccggctag
    18901 cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg atcgccagat gctgggcatt
    18961 acgttgtgcc agggagacag catcaggcat acgatctaca gcgataattt cgcagtccgg
    19021 gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc gtcccgagat cgagaatacg
    19081 gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc agacactccg tatccgggcg
    19141 cggaattaag gtcgctggcg aaacaaataa cggcaacgac cagaattctc gcaccccggt
    19201 taaatgagca atgggttcac catcgcgacg acgtgtcagt agcgcatcaa gttgctgaca
    19261 ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata aaagtacgcc ctctgccggt
    19321 aacatgctcc agcaggattt cagcatcacg ccgcgggctt tcgctcgcct gaagttggct
    19381 tattgcttca cgtaaccagt gttgatattc cattattcct gctcggacaa cgccgccagt
    19441 tggtcggcct gatgttcctg gataatcggt tcaatcagca tatccagctt accttccatc
    19501 acttcatcca ggcggtagag cgtcaggttg atgcggtgat cggtaacgcg cccctgcggg
    19561 aaattgtaag tacggttacg gtcgctgcga tcgccactcc ccagcaggtt acgacgggta
    19621 gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt ggatgcgagc accgagcact
    19681 gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt cctgacattc aacaacaatc
    19741 ccggtcggca agtgagtaat acgaattgcc gaatcggtgg tgttaacgtg ctgaccaccc
    19801 gcccctgacg agcggaaagt atcaatgcgt aaatctgctg ggttgatgtc cggcagttct
    19861 gcgtcaggca gttctggcat taccgcaacg gtacaagcag aagtatgaat acgaccctgc
    19921 gattccgtag caggaacacg ttgcacgcga tgaccgccgg attcaaattt cagacgacca
    19981 tacacaccat caccgctaat tttggcgatg atctctttat aaccaccatg ttcaccctcg
    20041 ctggcgctca tgatttctac ccgccagcgg cgggcttcgg cgtaacggct gtacatacgg
    20101 aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg ttccggcacg gacttcgagg
    20161 aaggcgttac gttcgtcatc aggatctttt ggtagtaata gaacctgtaa ttgctgttcc
    20221 agttgctcgc ttttttcttt agcttcgcgc agttcatcct gcgccatctc acgcatttca
    20281 ggatcgtcga gcatcatctg tgcggtttcg atatcttcct gaacctgttg ccagtcggta
    20341 aaacagcgcg aaacatcact taactgcgca tattcgcgtg ataatgcgcg aaaacgttcc
    20401 tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa cttcttcatg gcgttcatgc
    20461 agggcttcca gtttggcaac gatagaaggc ttcataggcg taaatgcacc ctgtaaaaaa
    20521 agaaaatgat gtactgctac tccagcccga ggctgtcgcg cagaatattc aggcgttcgt
    20581 tatccccgtc acgggcggcc tgttgaagtg atttcgttgg cgcatggatc aagcggttag
    20641 tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc gccgccctgc tcaagggccg
    20701 ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc ctggctgcga tactcgcgaa
    20761 tggtttcgct ggcgctttgt gctcgcagcc acgccataaa ttcgctggtt tcctgagcga
    20821 caatagtttc cgcctcaacc gctgcggctt tacgctgcgc caggttgtgc gaaatgatgc
    20881 tttgcagatc gtccacacta taaagataag cattcgccag tttgccaact tccggctcaa
    20941 catcgcgcgg aacggcaata tccaccaaca gcattggttg gttacgacga ctttttaatg
    21001 cgcgctccac catgcctttc ccgataatcg gtaacgggct ggcggtggaa ctgatgatga
    21061 tatcggcttc gcgcagacgt tcgtcgatct cactcagggc aatcacttcc gcgccaactt
    21121 catctgccag aatttgggca cgttcgcgag tgcggttggc gataatcatc ttctgtactt
    21181 tatgttcgcg cagatgacgc gctaccagct cgatggtttc gcccgcgcct accagcaaca
    21241 ctgtgaccgt agagagcgat tcaaagatct gccgcgccag cgtacaagcc gcaaaagcga
    21301 cagacacagc actggcaccg atatctgttt cagtgcgaac gcgtttcgct acagagaaag
    21361 atttctggaa catgcgttcc agttcgctgg ccttcatatg acctttttgc gaatcggcaa
    21421 acgctttttt aacctgaccg aggatctgcg gctccccaag aaccagtgaa tccaggccgc
    21481 tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg atgccagtag aggcttttac
    21541 gcagatcttc ttcattaaga ttgtgataat cgcaaagcca gcggattaac gcctcttgca
    21601 ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg gttgcacgtc gacagcacca
    21661 cgccgccctg caccatcggc tgcgcaagca ggctgtcaag cgcctgatcg agcttatccg
    21721 gcgaaaacga tacacgttct cgcagcgata caggtgccgt tttatggttg ataccgagtg
    21781 ctaaaagggt catgtctgcg ggaaataata ccaacgttga tagggttagt ctgcttgcat
    21841 catacaggat gcgtaagatc aataaaagag agcgccccct tttggagtaa ttgccgaaag
    21901 ccgttagatt ctggcaatta agacaacttg aacatagacg atagcggacg gtaacgctag
    21961 cattaagggt tataactgca acgtatctca aggacttgtc atcactatgc ccctgcccga
    22021 ttttcgtctt atccgcctgc taccgctggc tgctcttgtg ctcactgcct gttccgttat
    22081 cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa tggcgtcagc atcagcaaga
    22141 cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc gcttatattt ctgaccaaca
    22201 aaaagtgtac gcccgctttt tctggcagca aaccggccag gatcgctacc gtctgctgct
    22261 cactaaccca ttgggcagca cggaactgga gctgaatgct caaccgggta acgtgcagtt
    22321 agtcgacaat aaaggtcagc gttataccgc cgatgacgcc gaagagatga ttggcaaatt
    22381 gaccggaatg ccaattccgc tcaacagctt gcgccagtgg attttaggtt taccgggtga
    22441 tgcaaccgac tacaaactgg acgaccagta ccgcctgagc gaaattacct acagccagaa
    22501 tggcaaaaac tggaaggttg tttatggtgg ttatgacacc aaaacgcaac ctgcgatgcc
    22561 agccaatatg gaactcaccg acggtggtca acgcatcaag ttaaaaatgg ataactggat
    22621 agtgaaataa tgcggacaca gtggccctct ccggcaaaac ttaatctgtt tttatacatt
    22681 accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc tgtttcagtt tcttgattac
    22741 ggcgacacca tcagcattga gcttcgtgac gatggggata ttcgtctgtt aacgcccgtt
    22801 gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag cgcggttgtt gatgaaaact
    22861 gcggcagaca gcgggcgtct tccgacggga agcggtgcga atatcagcat tgacaagcgt
    22921 ttgccgatgg gcggcggtct cggcggtggt tcatccaatg ccgcgacggt cctggtggca
    22981 ttaaatcatc tctggcaatg cgggctaagc atggatgagc tggcggaaat ggggctgacg
    23041 ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg cgtttgccga aggcgttggt
    23101 gaaatactaa cgccggtgga tccgccagag aagtggtatc tggtggcgca ccctggtgta
    23161 agtattccga ctccggtgat ttttaaagat cctgaactcc cgcgcaatac gccaaaaagg
    23221 tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt gcgaggttat cgcaagaaaa
    23281 cgttttcgcg aggttgatgc ggtgctttcc tggctgttag aatacgcccc gtcgcgcctg
    23341 actgggacag gggcctgtgt ctttgctgaa tttgatacag agtctgaagc ccgccaggtg
    23401 ctagagcaag ccccggaatg gctcaatggc tttgtggcga aaggcgttaa tctttcccca
    23461 ttgcacagag ccatgcttta agccgggcaa gctgagtttc ggtgacaacg tcaccttgtt
    23521 ccagacgttg catcgcgctc tttaatacac cgcttggaaa ggatcatgcc aggcccgcac
    23581 agttttcggc agattctttc caccaatgga cgcatgcctg aggttcttct cgtgcctgat
    23641 atgaagcttt ttgctggtaa cgccaccccg gtactagcac aacgtattgc caaccgcctg
    23701 tacacttcac tcggcgacgc cgctgtaggt cgctttagcg acggcgaagt cagcgtacaa
    23761 attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc agtccacttg tgcccctact
    23821 aacgacaacc tgatggaatt agtcgttatg gttgatgccc tgcgtcgtgc ttccgcaggt
    23881 cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc aggaccgtcg cgtccgttcc
    23941 gctcgtgtac caatcactgc gaaagtggtt gcagacttcc tctccagcgt cggtgttgac
    24001 cgtgtgctga cagtggatct gcacgctgaa cagattcagg gtttcttcga cgttccggtt
    24061 gataacgtat ttggtagccc gatcctgctg gaagacatgc tgcagctgaa tctggataac
    24121 ccaattgtgg tttctccgga catcggcggc gttgtgcgtg cccgcgctat cgctaagctg
    24181 ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc cgcgtgcgaa cgtttcccag
    24241 gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg tactggtcga tgatatgatc
    24301 gacactggcg gtacgctgtg taaagctgct gaagcgctga aagaacgtgg tgctaaacgt
    24361 gtatttgcgt acgcgactca cccgatcttc tctggcaacg cggcgaacaa cctgcgtaac
    24421 tctgtaattg atgaagtcgt tgtctgcgat accattccgc tgagcgatga aatcaaatca
    24481 ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg ccgaagcgat tcgtcgtatc
    24541 agcaacgaag aatcgatctc tgccatgttc gaacactaat cgaacccggc tcaaagaccc
    24601 gctgcggcgg gtttttttgt ctgtaatatc catttgtatg acctatgcct ccttcacctg
    24661 ccatttagtt gacagatgat gcgctcacgg atgaaacatt attgtgaaca aaatattttc
    24721 ctcacatgtg atgcctttcc gcgctctgat cgacgcttgc tggaaagaaa aatatactgc
    24781 cgcacggttt acccgtgacc tgattgccgg gataaccgtc gggattattg ctatcccgct
    24841 ggcgatggcg ttggctattg gtagtggtgt ggcaccccag tacggtttat ataccgcagc
    24901 tgttgcgggg attgtcattg ctctaacggg tgggtcacgc tttagcgttt ccggtccgac
    24961 tgcggcattt gtggtaattc tctatcccgt ttcgcaacag tttggactgg caggactgct
    25021 ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg gggctggcac gctttggtcg
    25081 tctgattgag tatattccgg tttccgtcac cttaggtttc acctcgggta tcgggatcac
    25141 catcggtacc atgcagatta aagattttct cggtctgcaa atggcccatg tcccggaaca
    25201 ttatctacaa aaagtcggcg cattatttat ggcgctgccg accattaatg tgggtgatgc
    25261 tgccattggc attgtgacgc ttggcattct ggttttctgg ccgcgtctgg gcattcgttt
    25321 acccggtcac cttccggcct tgctggctgg ttgcgcggtg atggggattg ttaacctgct
    25381 cggcggacat gttgctacca tcggttcgca attccactac gtcctggccg atggttctca
    25441 gggtaacggt attccgcaac tgctgccgca actggtgctg ccgtgggatc tgcctaattc
    25501 agaattcacg ctaacctggg attctattcg caccctgctg cctgcggcat tctcaatggc
    25561 aatgctcggg gcgatcgaat ctctgctctg cgctgtggta ctggatggta tgaccgggac
    25621 taagcacaag gcgaatagtg aactggtcgg tcaggggctg gggaatatca tcgctccatt
    25681 ctttggtggt attaccgcta ctgctgccat cgcgcgttct gccgctaacg tccgtgccgg
    25741 ggcaacttcc cctatctcgg cggtgatcca ctctactctg gttattctcg ccctgctggt
    25801 actggcacca ctgctctcct ggctgccgct ttccgccatg gcagccctgc tgttgatggt
    25861 ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg ctgcgtcatg cgccgaaaga
    25921 tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg ctgtttgata tggttattgc
    25981 catcagcgtg gggatcgtgc tggcatcgct gctgtttatg cgtcgtatcg cacgtatgac
    26041 tcgcctggca ccggtagtcg tagatgttcc agacgatgtc ctggttctgc gcgttattgg
    26101 cccgctgttt tttgctgctg ctgaaggctt attcacggac ctagagtcac gtcttgaagg
    26161 caaacggatt gtgattctga agtgggatgc cgttccggta cttgatgctg gtggtcttga
    26221 tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt gaactgcgcg tgtgcaacgt
    26281 ggaattccag ccactgcgaa ctatggctcg cgcaggcatt caaccgatcc cgggacgcct
    26341 cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta taagtacgag attgaccagt
    26401 cagcgcaaac tgactggtca gcaaactgca ttatttgtta gctatgacgg cgattatcgc
    26461 tacggcggca acgtttgtca tagtgacgaa cccaccagta acggtcgcac acctgttcat
    26521 gcccgccaac acgggcaccc gccagccaga gaatggcacc gacgaaaata cttagcactg
    26581 caccatgtgc gaaatactgg ggcatattaa actgtggtaa ctggttgagg attgaatacc
    26641 ccacgccgac caccattacc accagaccca accccatgag cacgttaccg agtatcgaag
    26701 cgtttttgcg tttcatatgt cacctccgga actttctggg ttgtaacagg gaatacccct
    26761 cttccttatg tgtaaagtat agacaacacc ctgtgggtta tgtgcgggcg taatcacaat
    26821 tacaaccctt atttcaacaa aactttacaa ataaacgcct gaacaccttg actttctaat
    26881 agtccactca ggcaattatt acgttatctg catttgatac gcagtttttt gtcaagcggg
    26941 gccgcaacca gtaaactacg cgccagttat gtacacactc aggacaaaaa aacgtgacga
    27001 ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata cgccgcaacg cgacataatg
    27061 ctggtgcctg gttcgttgac ttactggcag agcgtttgcg cgctccgctg cgcgaagagg
    27121 ctaaattctt tggttatact tcgcgagtca cccttggagg cgaagatgtc cgcctgttag
    27181 tcccgactac atttatgaat ctcagcggca aagccgttgc ggcgatggcc agttttttcc
    27241 gcattaatcc ggacgaaatt ctggtggccc acgacgaact ggatctgcct cctggcgtcg
    27301 ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg actgaaagac atcatcagta
    27361 aattgggtaa taaccctaac tttcaccgtt tacgcatcgg aatcggtcat ccgggcgata
    27421 aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt tagtgaacag aagttaattg
    27481 atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg gtttacagat ggcttgacca
    27541 aagcaacgaa ccgattgcac gcctttaaag cgcaataagt cgttgtctgc ggcatttttg
    27601 ccgagtgccg tgtataatag gcaaagttat ttccatttct gcaatctgtt agcaataaca
    27661 ggttgattat taagatatta aggtgattta aatcatggga ttcaaatgcg gtatcgtcgg
    27721 tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg accaaagccg gtattgaagc
    27781 ggccaacttt ccattctgca ccattgagcc gaacacaggc gtcgtaccaa tgcctgaccc
    27841 tcgcctggat caactggctg aaatcgtaaa accgcaacgt acgcttccca cgaccatgga
    27901 atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa ggcgaaggtc tgggtaacca
    27961 gttcctgacc aacatccgtg aaaccgaagc gatcggtcac gttgttcgct gctttgaaaa
    28021 tgacaacatc attcacgttt ccggcaaagt taacccggct gacgatattg aagttatcaa
    28081 caccgaactg gcgctggcag acctcgacac ctgcgaacgt gcgattcatc gcgtacagaa
    28141 gaaagccaaa ggtggcgata aagacgcgaa agctgagctg gcggtcctgg aaaaatgcct
    28201 tccccagctg gaaaacgcag gtatgctgcg cgcgctggat ttaagcgctg aagagaaagc
    28261 ggctattcgt tacctgagct tcctgacgct gaaaccaaca atgtacatcg ccaacgtcaa
    28321 cgaagacggt tttgaaaaca acccatatct tgaccaggtg cgtgaaatcg cggcgaaaga
    28381 aggttctgtt gtggttccgg tttgtgctgc tgttgaagca gacattgccg aactggacga
    28441 cgaagaacgt gacgagttta tgcaggagct tgggctggaa gaaccgggcc tgaaccgtgt
    28501 gatccgtgcc ggttataagc tgctgaacct gcaaacttac ttcaccgctg gggtgaaaga
    28561 agtgcgtgca tggaccattc cggttggagc aaccgcgccg caggcagcgg gcaaaatcca
    28621 tactgatttt gaaaaaggct ttatccgtgc acaaaccatc tcgtttgaag atttcatcac
    28681 ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg cgtgcagaag gtaaagatta
    28741 catcgtgaaa gatggcgatg tgatgaactt ccttttcaac gtctaattaa tttattgtct
    28801 catgaggttt cgctaaatct catgaagagc gcaaaagtat ataaaatcca cgcaattgcg
    28861 tggatttttc atttcaccga gtcctaatca ttcttgtaac atcctacact tttgtgctta
    28921 ttgcctacag tgaaccacga gaataattaa agacataagt gttcagatga ctgatatctc
    28981 attagcagtg atataacgtc agtttttgtt ccactacaca tcagccctct tcacagctga
    29041 tcaatattga tctcgctcca atatgctggc gagaaaagga cttacctgaa gggtaatatc
    29101 ccgcagataa agtccccgaa taacgtgatt acgtgcgatc ctggcaagat gaaaatgata
    29161 accagta
//
LOCUS       DBKEKI010000043        29024 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.043, whole genome shotgun sequence.
ACCESSION   DBKEKI010000043 DBKEKI010000000
VERSION     DBKEKI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.043"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..213)
                     /locus_tag="ACUQPX_001842"
     CDS             complement(<1..213)
                     /locus_tag="ACUQPX_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HHV9056569.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="ACUQPX_001843"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="ACUQPX_001843"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HHV9056570.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQSEKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="ACUQPX_001844"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="ACUQPX_001844"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HHV9056571.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="ACUQPX_001845"
     CDS             2894..3634
                     /locus_tag="ACUQPX_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHV9056572.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="ACUQPX_001846"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="ACUQPX_001846"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HHV9056573.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQAGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4192..4794)
                     /locus_tag="ACUQPX_001847"
     CDS             complement(4192..4794)
                     /locus_tag="ACUQPX_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HHV9056574.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4865..4930
                     /gene="yobI"
                     /locus_tag="ACUQPX_001848"
     CDS             4865..4930
                     /gene="yobI"
                     /locus_tag="ACUQPX_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HHV9056575.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5069..5680
                     /gene="ruvA"
                     /locus_tag="ACUQPX_001849"
     CDS             5069..5680
                     /gene="ruvA"
                     /locus_tag="ACUQPX_001849"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HHV9056576.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            5689..6699
                     /gene="ruvB"
                     /locus_tag="ACUQPX_001850"
     CDS             5689..6699
                     /gene="ruvB"
                     /locus_tag="ACUQPX_001850"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HHV9056577.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6846..7631)
                     /gene="znuB"
                     /locus_tag="ACUQPX_001851"
     CDS             complement(6846..7631)
                     /gene="znuB"
                     /locus_tag="ACUQPX_001851"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HHV9056578.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7628..8383)
                     /gene="znuC"
                     /locus_tag="ACUQPX_001852"
     CDS             complement(7628..8383)
                     /gene="znuC"
                     /locus_tag="ACUQPX_001852"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HHV9056579.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8462..9394
                     /gene="znuA"
                     /locus_tag="ACUQPX_001853"
     CDS             8462..9394
                     /gene="znuA"
                     /locus_tag="ACUQPX_001853"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HHV9056580.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9410..10732
                     /gene="mepM"
                     /locus_tag="ACUQPX_001854"
     CDS             9410..10732
                     /gene="mepM"
                     /locus_tag="ACUQPX_001854"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HHV9056581.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10852..11823
                     /gene="lpxM"
                     /locus_tag="ACUQPX_001855"
                     /gene_synonym="msbB"
     CDS             10852..11823
                     /gene="lpxM"
                     /locus_tag="ACUQPX_001855"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HHV9056582.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11954..13396)
                     /gene="pyk"
                     /locus_tag="ACUQPX_001856"
     CDS             complement(11954..13396)
                     /gene="pyk"
                     /locus_tag="ACUQPX_001856"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HHV9056583.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13524..14393)
                     /gene="hexR"
                     /locus_tag="ACUQPX_001857"
                     /gene_synonym="yebK"
     CDS             complement(13524..14393)
                     /gene="hexR"
                     /locus_tag="ACUQPX_001857"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HHV9056584.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14730..16205
                     /gene="zwf"
                     /locus_tag="ACUQPX_001858"
     CDS             14730..16205
                     /gene="zwf"
                     /locus_tag="ACUQPX_001858"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HHV9056585.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16440..18251
                     /gene="edd"
                     /locus_tag="ACUQPX_001859"
     CDS             16440..18251
                     /gene="edd"
                     /locus_tag="ACUQPX_001859"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HHV9056586.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18288..18929
                     /gene="kdgA"
                     /locus_tag="ACUQPX_001860"
                     /gene_synonym="eda"
     CDS             18288..18929
                     /gene="kdgA"
                     /locus_tag="ACUQPX_001860"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HHV9056587.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18985..20163)
                     /gene="purT"
                     /locus_tag="ACUQPX_001861"
     CDS             complement(18985..20163)
                     /gene="purT"
                     /locus_tag="ACUQPX_001861"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HHV9056588.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20297..20587
                     /gene="yebG"
                     /locus_tag="ACUQPX_001862"
     CDS             20297..20587
                     /gene="yebG"
                     /locus_tag="ACUQPX_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HHV9056589.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            20617..21009
                     /gene="yebF"
                     /locus_tag="ACUQPX_001863"
     CDS             20617..21009
                     /gene="yebF"
                     /locus_tag="ACUQPX_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HHV9056590.1"
                     /translation="MLSPESLCYGEKHEKRGAFLGRLLVSACASVFAANNETSKSVTF
                     PKCEGLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVP
                     LTVRGKSADIHYQVSVDCKAGMAEYQRR"
     gene            21336..21995
                     /locus_tag="ACUQPX_001864"
     CDS             21336..21995
                     /locus_tag="ACUQPX_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HHV9056591.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQVIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22204..24264
                     /gene="ptrB"
                     /locus_tag="ACUQPX_001865"
     CDS             22204..24264
                     /gene="ptrB"
                     /locus_tag="ACUQPX_001865"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HHV9056592.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQGPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24261..24923)
                     /gene="exoX"
                     /locus_tag="ACUQPX_001866"
     CDS             complement(24261..24923)
                     /gene="exoX"
                     /locus_tag="ACUQPX_001866"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HHV9056593.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPRLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24947..25603)
                     /gene="yobB"
                     /locus_tag="ACUQPX_001867"
     CDS             complement(24947..25603)
                     /gene="yobB"
                     /locus_tag="ACUQPX_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HHV9056594.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIPGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(25705..25935)
                     /gene="holE"
                     /locus_tag="ACUQPX_001868"
     CDS             complement(25705..25935)
                     /gene="holE"
                     /locus_tag="ACUQPX_001868"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HHV9056595.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26088..26447
                     /gene="yobA"
                     /locus_tag="ACUQPX_001869"
                     /pseudo
     CDS             26088..26447
                     /gene="yobA"
                     /locus_tag="ACUQPX_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA];
                     GO_process: GO:0046688 - response to copper ion [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
     gene            26451..27323
                     /gene="copD"
                     /locus_tag="ACUQPX_001870"
     CDS             26451..27323
                     /gene="copD"
                     /locus_tag="ACUQPX_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HHV9056596.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLMIRRLLTKRSL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            27336..27677
                     /locus_tag="ACUQPX_001871"
     CDS             27336..27677
                     /locus_tag="ACUQPX_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HHV9056597.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28070..>29024)
                     /locus_tag="ACUQPX_001872"
     CDS             complement(28070..>29024)
                     /locus_tag="ACUQPX_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9056598.1"
                     /translation="RDAVTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKT
                     ILIRRKVSRNTYKIRANQLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSM
                     RSVLSDMFREAIVEGRISQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPAWFPL
                     AMDLALVTGQRREDITNMRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVV
                     ERCRLVSRGDYLISAGIRKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRS
                     LAGRLYKETCGEEFAQRLLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT         7332 a         7146 c         7489 g         7057 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc gcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agagcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgcggcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggcaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctgg cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagatacg aatatacgta atagcataaa aaaattattt tttcaggagt
     4861 cgcaatgtat atttttatta cgcatttctt cactgaatat gtaatattca aatatttgct
     4921 tccaatataa cctgttgaat aaattatact gtgccatttt tcagatcatc gggacacctc
     4981 gcaagttttc ttcatccttc gctggatatc tatccagcat ttttttatca tacagcatta
     5041 tctttgattc attacgcagg agcgtcatgt gataggcaga ctcagaggca tcatcattga
     5101 aaaacaaccc ccgctggtgt taattgaagt gggcggcgta ggctatgaag tgcatatgcc
     5161 gatgacctgt ttttatgaac tccctgaagc gggtcaggaa gcgatcgttt tcacccactt
     5221 tgtggtgcgt gaagacgcgc aactgctgta cggttttaac aataaacaag aacgcacgtt
     5281 gttcaaagag ttgatcaaaa ccaacggcgt cggcccgaag ttggccctgg cgatcctctc
     5341 cggaatgtca gcgcagcagt tcgttaatgc cgttgagcgt gaagaagtgg gggcactggt
     5401 gaaactgccg ggtattggca aaaaaaccgc cgaacgcttg attgttgaaa tgaaagaccg
     5461 atttaaaggt ttgcatggcg atctctttac gccagccgcc gacctggtac tcacgtctcc
     5521 tgccagcccg gcgaccgacg atgctgaaca agaagcagtt gccgcgctgg tggcgctagg
     5581 ctataaacca caagaagcaa gccgcatggt gagcaaaatc gctcgccctg acgccagtag
     5641 tgaaacatta attcgcgaag ccctacgcgc cgcgttatga ggtaaaggat gattgaagca
     5701 gaccgtctga tttctgccgg taccactttg ccggaagatg tagcagatcg cgccattcgc
     5761 cctaaattac tggaagagta tgttggtcag ccgcaggttc gttcacagat ggagattttc
     5821 atcaaagcag cgaaactgcg cggcgatgcc ctcgatcatt tgttgatttt tggtcctccg
     5881 gggttgggta aaactacgct tgccaacatt gtcgccaatg aaatgggcgt taatttacgc
     5941 acgacttctg gtccggtgct ggaaaaggcg ggcgatttgg ctgcgatgct cactaacctt
     6001 gaaccgcatg acgtgctgtt tattgatgag atccaccgtt tatcgccagt tgttgaagaa
     6061 gtgctgtacc cggcaatgga agactaccaa ctggatatca tgattggtga aggtccggcg
     6121 gcacgctcca ttaaaattga tttgccgccg tttaccctga ttggcgcaac cacgcgcgca
     6181 ggttcgctga catcaccgtt gcgcgaccgt tttggtattg tgcaacgtct ggagttttat
     6241 caggtgccgg atctgcaata tatcgtcagt cgcagtgcac gctttatggg gcttgagatg
     6301 agtgatgacg gtgcgctgga agtagctcgt cgcgctcgcg gtacgccacg tattgccaac
     6361 cgtctgctgc gtcgagtgcg tgatttcgcc gaagtgaagc acgatggcac catctcggca
     6421 gatatcgctg ctcaggcgct ggatatgttg aatgtcgatg ctgaaggttt cgattatatg
     6481 gaccgcaaat tgttgctggc ggtaatcgat aagttctttg gtgggccggt aggtctggat
     6541 aacctggcgg cagccattgg cgaagaacgt gaaaccattg aagatgtact ggaaccttat
     6601 ttgattcagc aaggcttttt gcagcgtaca ccgcgcgggc gtatggcgac gacgcgggcg
     6661 tggaatcact ttggcataac gccgccagaa atgccgtaag tcggattggt cgctcgcgcc
     6721 gcatccgaca ctggcagtgt gtctgatgcg acactgttcg cgtcttatca ggccagccag
     6781 agatagcgaa ccgtaggtcg gataaggcgc tcgcgccgca tccgacaaat gtgttcagcg
     6841 atagattagc tggcctgctt tttcatcata ctgataataa ataacagtgc cgcacatagc
     6901 accaccgacg gacccgccgg cgtatcgtaa aacgcggaaa aggttaaacc gccagttact
     6961 gccaccatcc ccaccaaaac agcgacacca gccatctgtt ccggcgtacg ggcaaagcga
     7021 cgcgcagtag ctgcaggaat aatcagcaac gaagtaataa tcaacgcacc gacaaatttc
     7081 atcgctacac caatcgtcaa tgccgtcacc agcatcaaca acaatttcac ttgctgtaat
     7141 ttcacaccat caacaaacgc cagatccggg ctaatcgtca tcgacagcaa attgcgccat
     7201 tgccagaaca aaatagccac cacgatgacc acgccaatcg caatagagat gagatcttct
     7261 ggcgtcactg ccagtaaatc accgaacagg taagccatca aatcaacacg aatgttagac
     7321 atcagactaa cgaccactag gcccagcgac agggcactgt gcgccataat ccctaataac
     7381 gtgtcgatcg ccagctgtgg acgcttctcc agccatacca gaccgcccgc cagcagcagc
     7441 gtaacggcaa tcaccgcata gaatggattc acgtccagca acaaaccaaa cgcgacacca
     7501 agtaatgagg catgagccag cgtatcaccg aaataagaca tacgacgcca gactacaaac
     7561 gaacccagcg gacccgcggc acaggcgagc atgatcccgg ctaaccaacc gggaaataat
     7621 aattcaatca tgagcgatca tttccccgac gcaaaacaat tcgtccctgt aaatcgtgac
     7681 gatgattatg atgatggcga tagataccca gttgttcagc accacgagga ccaaacattg
     7741 aaataaactc cggatgcagg gaaacaactt ccggtgtgcc ggaacaacaa atgtggtgat
     7801 tcaggcacag cacttcatcg gtttttgcca tcaccagatg cagatcgtga gaaaccatta
     7861 aaacgccaca atccagttca cgacgcaact ggtcaataag gtcatataac gccacttggc
     7921 catttacatc cacgccttga gtgggttcat ccagcactaa taattgcggg caatttaaca
     7981 atgctcgcgc taatagtaca cgctgcgttt cgccaccaga gagcttttgc atcggtgcgt
     8041 taatcaggtg cccggcctgg acacgtttca gtgcaggcaa aatatcttct ttatgtgtac
     8101 cagggcgtaa gcgtaaaaaa cggtttacgg tcagtggcaa cgtggtgtcg agatacagct
     8161 tctgcggtac atagccgatg cgcagttttc cgttgcgctt gataacccct tcatcgggtg
     8221 ttaccagccc gagcactacc cgtaccagtg tcgacttacc tgcgccattt ggcccaagta
     8281 aagtcaaaat ttttccaggt ttaagttcca gcgacacatc agagaggacg cggcgttggc
     8341 caaaagaaac cgagacattt tccagggaaa ccagacttgt catgttaatt ttagtcttgc
     8401 agtagtcatg aaatgttata atatcacact tctcatattc attacgatga ttggtcgcat
     8461 tatgttacat aaaaaaacgc ttcttttcgc agcattatcc gccgctctct ggggaggtgc
     8521 aacacaggcc gcagatgccg ccgttgtcgc ttcgcttaaa cccgttgggt tcatcgcttc
     8581 tgccattgct gatggtgtaa cagaaacgga ggttttactg cctgacggcg cttcagaaca
     8641 tgattattca ctgcgtccat cggatgtaaa acgcttacag aacgcggact tagtcgtttg
     8701 ggttggcccg gagatggaag cgtttatgca aaaaccggta agcaaattac cagaagcgaa
     8761 gcaggtaacg attgcgcagc ttgaagatgt gaaaccgcta ctgatgaaaa gtattcacgg
     8821 cgatgatgat gatcacgacc acgcggaaaa aagtgacgaa gatcaccatc acggcgattt
     8881 caacatgcat ctttggcttt ccccagagat agcgcgggct acagcggttg caatccatgg
     8941 aaaattagtg gaacttatgc cgcaaagtcg agccaaactt gacgccaacc tgaaggattt
     9001 tgaggcacaa ttggcctcaa ccgaaacgca ggttggtaac gagctcgcgc cgctcaaggg
     9061 gaaaggttat ttcgtttttc acgatgctta cggctatttc gaaaaacagt tcggactgac
     9121 accgcttggt cattttaccg ttaaccctga gattcaacct ggcgcgcagc gtttacatga
     9181 aataagaaca cagttggttg agcaaaaagc aacctgcgtt tttgctgagc cacagttcag
     9241 gccagcggtc gttgagagcg tcgcacgagg gacatccgtt cgtatgggaa cgttggatcc
     9301 cctggggacg aatatcaaac tgggtaaaac aagctattca gaattcctga gtcaattagc
     9361 caaccagtat gcgagctgcc tgaaaggaga ttaatgagga agtgattacg tgcaacagat
     9421 agcccgctct gtcgccctgg cgtttaataa tttaccgcga ccacaccgcg ttatgttggg
     9481 gtcgctcacc gttcttactc tggccgtcgc tgtctggcgg ccctatgttt atcaccgtga
     9541 tgccacgcca attgtcaaaa ccattgagct ggaacagaac gaaattcgtt cgctcttacc
     9601 tgaagccagt gagccgattg atcaagctgc acaagaagat gaagccattc cccaggacga
     9661 actggatgac aaaatcgccg gtgaagcggg cgtgcatgaa tatgttgttt ccactggcga
     9721 tacgctaagc agcattctca atcagtatgg tattgatatg ggtgatatca cccaactggc
     9781 tgctgccgac aaagaattgc gtaacctgaa aatcggtcaa caactctcct ggacattaac
     9841 cgcggacggc gaactgcagc gcctcacctg ggaagtgtct cgtcgtgaaa cccgaaccta
     9901 tgaccgtact gccgctaacg gttttaaaat gaccagcgaa atgcagcaag gagagtgggt
     9961 taacaatctg ctgaaaggta ccgtcggagg gagctttgtt gccagcgcca gaaacgccgg
    10021 tttaaccagc gccgaagtga gcgcagtgat taaagccatg cagtggcaaa tggatttccg
    10081 caaactgaaa aaaggcgatg aatttgcggt attaatgtct cgagaaatgc ttgatggtaa
    10141 acgtgagcaa agccagctgc tgggcgtacg tttgcgttca gaaggtaaag attattacgc
    10201 aatccgcgct gaggatggca aattctacga tcgtaacggt actggtctgg cgaaaggatt
    10261 cttgcgattc ccgacggcga aacagttccg tatctcgtct aactttaacc cgcgtcgaac
    10321 taatccggtg acaggtcgcg ttgcaccaca cagaggtgtt gatttcgcca tgccacaagg
    10381 tacgccagtg ctttcagtgg gtgacggtga agtggtggtt gccaaacgca gtggcgcagc
    10441 aggttattat gtggctattc gtcatggtcg cagctacacc acgcgttata tgcacttgcg
    10501 caagattctg gtgaaaccgg gacagaaggt gaaacgtggc gaccgtatcg cgctttccgg
    10561 taataccgga cgttcaactg ggccgcatct gcactatgaa gtatggataa accagcaggc
    10621 cgtaaacccg ctgacggcaa aactgccgcg taccgaaggg ctgaccggct ccgatcgtcg
    10681 cgaattcctg gcgcaggcca aagagattgt gccgcagcta cggtttgatt aattaacatc
    10741 cattcgcagc cggtacgcag tcagtaccgg ctttttttat ttggtgcggg gcaagttgcg
    10801 ccgctacact atcaccagat tgatttttgc cttatccgaa actggaaaag catggaaacg
    10861 aaaaaaaata atagcgaata cattcctgag tttgataaat cctttcgcca cccgcgctac
    10921 tggggagcat ggctgggtgt agcagcgatg gcgggtatcg ctttaacgcc gccaaagttc
    10981 cgtgatccca ttctggcacg gctgggacgt tttgccggac gactgggaaa aagctcacgc
    11041 cgtcgtgcgt taatcaatct gtcgctctgc tttccagaac gtagtgaagc tgaacgcgaa
    11101 gcgattgttg atgagatgtt tgccaccgcg ccgcaagcga tggcaatgat ggctgagttg
    11161 gcaatacgcg ggccggagaa aattcagccg cgcgttggct ggcaagggct ggagatcatc
    11221 gaagagatgc ggcgtaataa cgagaaagtt atctttctgg tgccgcacgg ttgggccgtc
    11281 gatattcctg ccatgctgat ggcctcgcaa gggcagaaaa tggcagcgat gttccataat
    11341 cagggcaacc cggtttttga ttatgtctgg aacacggtgc gtcgtcgctt tggcggtcgt
    11401 ctgcatgcga gaaatgacgg tattaaacca ttcatccagt cggtacgtca ggggtactgg
    11461 ggatattatt tacccgatca ggatcatggc ccagagcaca gcgaatttgt ggatttcttt
    11521 gccacctata aagcgacgtt gcccgcgatt ggtcgtttga tgaaagtatg ccgtgcgcgc
    11581 gttgtaccgc tgtttccgat ttatgatggc aagacgcatc gtctgacgat tcaggtgcgc
    11641 ccaccgatgg atgatctgtt agaggcggat gatcatacga ttgagcggcg gatgaatgaa
    11701 gaagtcgaga tttttgttgg tccgcgacca gaacaataca cctggatact aaaattgctg
    11761 aaaactcgca aaccgggcga aatccagccg tataagcgca aagatcttta tcccatcaaa
    11821 taaaaaagcc tctcgcgagg agaggccttc gcctgatgat aagttcaagt ttgcttcaga
    11881 atattcgaaa tctgttgaac tatcattgaa ctataggccg gatgtggcgt tttcgccgca
    11941 tccggcaacg tacttactct accgttaaaa tacgcgtggt attagtagaa cccacggtac
    12001 tcatcacgtc gccctgggtg acaatcacca ggtcaccaga catcaagtaa cctttatcgc
    12061 gcagcagatt aaccgcttcg ctggcagctg ctacgccgtc attagcgcta tcaaagtgca
    12121 ccggcgtaac gccacgatag agagcagtca ggttcagcgt acgttcatgg cgcgacatgg
    12181 cgaaaattgg tagaccagag ctgatacggg aggtcatcag cgcggtacga cccgattcgg
    12241 tcatggtaat gatcgccgta acgcccttca ggtggttagc tgcgtacatt gccgacatgg
    12301 caatagcttc ttccacattg tcgaactgaa cgtccagacg gtgtttagaa acgttgatgc
    12361 tcgggatttt ttccgcaccc aggcaaacgc gcgccatggc tgcaacagtt tctgacggat
    12421 actgcccagc ggcagtttct gcagacagca tcacagcgtc agtaccatcc agaacggcgt
    12481 ttgctacgtc catgacttct gcacgcgtcg gcatcgggtt agtaatcatt gactccatca
    12541 tctgggtcgc cgtgattacc gctcggttta gctgacgcgc acgacggatc aacgctttct
    12601 gaatgccgac cagttccggg tcgccaattt ccacaccgag gtcgccacgt gcaaccatta
    12661 ccacgtcaga ggcgaggatg atgtcatcca ttgcatcctg gctgcaaacg gcttccgcac
    12721 gttcaacctt ggcaacaatt ttcgcatcac atcctgcatc gcgtgccagg cgacgggcat
    12781 agttcagatc ttcgccacag cgtgggaagg agacagccag gtaatctacg ccaatcaacg
    12841 ccgcagtctt aatgtctgct ttgtcttttt cggtcagcgc ttcagccgac aaaccgccgc
    12901 caagtttgtt gataccttta ttgttggaaa ggggaccacc gacggtgact tcggtgaaca
    12961 ctttcatgcc ctgaacttcc agtactttta actggacgcg accatcgtcc agcagcagga
    13021 tgtcaccagg cacgacgtca gcaggcaggc ctttgtagtc gataccgact ttttctttgt
    13081 cgccttcacc tttacctagg ttggcgtcga gcaggaattt atccccaata ttgaggaaaa
    13141 ctttgccttc tttaaaggtg gatacacgga ttttgggccc ctggaggtca cccagaatag
    13201 ccacatgacg ccccagtttt gcggcaatct cacgaacttt atccgcgcgc attttgtgat
    13261 cttcaggcga gccgtgagaa aagttcatac gtacaacgtt ggcacccgcc gcgataactt
    13321 tttcaagatt attatcgcga tctgttgctg ggcctaacgt ggtaacgatt tttgttctgc
    13381 gaagcctttt ggacatgtaa tactccgttg actgaaacaa ccaggtgttg cttgaacatg
    13441 aaatccgaat gaaataacgc cgcgatggcg ataaattaat ctcattcgga tgtcgtataa
    13501 gattaggaca gtgacagtcg cttttagcga tcgtcactta aattaagtaa ctgcttatca
    13561 aaacgcgatt ccttcagcgc ttccttgacc cgcttcaagt tatctctgaa ttttgcaccg
    13621 cgtcgcaaag taaatcctgt cgccagcaca tctatcacgg tcagctgtgc aagtcgagaa
    13681 accatgggca tataaatgtc agtatcttcc ggtacgtcga gggtaattgc cagcgttgct
    13741 tcccgggcga gcggggtacc cgcagaggtg agggcaatca ccatggcgtc gttttcgcgt
    13801 gccagctgcg ccagctcgac cagatttttt gttcttcctg tgtgagaaat cagcaccacc
    13861 acgtctccgt cgctacaatt catacaactc atgcgttgca gcacgatatc atcggagtac
    13921 accaccggaa cattaaaacg aaagaactta ttcatcgcat cgtgggcaac ggcggcagaa
    13981 gagcctaatc cgaaaaaggc gatttttttt gcctgagtga gcaagtcgac ggcgcggttg
    14041 atggcagatt tatccagtga atgacggaca tgatcgagcg ttgccattgc ggactcaaat
    14101 attttccccg tgtatgattc aacgctgtca tcttcattga cgttgcgatt aacataggga
    14161 gtgccattcg ccagactttg tgccagatga agtttaaaat caggaaaacc gcgcgtgtcc
    14221 atgctgcgac agaaacgatt caccgtcggt tcgctaacat tggcttccag cgccagagca
    14281 gcaatactcg aatggatcgc gttatcgggc gaagccagaa tgacctcggc aactttgcgc
    14341 tctgatttgc tcaaatgttc cagctgagac tggatttttt ccagcatatt catgatgtaa
    14401 agagactcac gggtaatgac gatttccgca ctgaaagaaa tcgaaatgca gttttgtcag
    14461 atattacgcc tgtgtgccgt gttaatgaca aaagcagata aaaaagttgt tatttttttc
    14521 ataacatgat cagtgtcaga tttttaccga atggaaaacg atgatttttt tatcagtttt
    14581 gccgcacttt gcgcgctttt cccgtaatcg cacgggtgga taagcgttta cagttttcgc
    14641 aagctcgtaa aagcagtaca gtgcaccgta agaaaattac aagtataccc tggcttaagt
    14701 actgggttag ttaacttaag gagaatgaca tggcggtaac gcaaacagcc caggcctgtg
    14761 acctggtcat tttcggcgcg aaaggcgacc ttgcgcgtcg taaattgctg ccttccctgt
    14821 atcaactgga aaaagccggt cagctcaacc cggacacccg gattatcggc gtagggcgtg
    14881 ctgactggga taaagcggca tataccaaag ttgtccgcga ggcgctcgaa actttcatga
    14941 aagaaaccat tgatgaaggt ttatgggaca ccctgagtgc acgtctggat ttttgtaatc
    15001 tcgatgtcaa tgacactgct gcattcaacc gtctcggcgc gatgctggat caaaaaaatc
    15061 gtatcaccat taactacttt gccatgccgc ccagcacttt tggcgcaatt tgcaaagggc
    15121 ttggcgaggc aaaactgaat gctaaaccgg cacgcgtagt catggagaaa ccgctgggga
    15181 cgtcgctggc gacctcgcag gaaatcaatg atcaggttgg cgaatacttc gaggagtgcc
    15241 aggtttaccg tatcgaccac tatcttggta aagaaacggt gctgaacctg ttggcgctgc
    15301 gttttgctaa ctccctgttt gtgaataact gggacaatcg caccattgat catgttgaga
    15361 ttaccgtggc agaagaagtg gggatcgaag ggcgctgggg ctattttgat aaagccggtc
    15421 agatgcgtga catgattcag aaccacctgc tacaaattct ctgcatgatt gcgatgtctc
    15481 cgccatctga cctgagcgca gacagcatcc gcgatgaaaa agtgaaagta ctgaagtccc
    15541 tgcgccgcat cgaccgctcc aacgtacgcg aaaaaaccgt acgcgggcaa tatactgcgg
    15601 gcttcgccca gggcaaaaaa gtgccgggat atctggaaga agagggcgcg aacaaaagca
    15661 gcaatacaga aaccttcgtg gcgatccgcg tcgacattga taactggcgc tgggccggtg
    15721 tgccattcta cctgcgtact ggtaaacgtc tgccgaccaa atgttctgaa gtcgtggtct
    15781 atttcaaaac acctgaactg aatctgttta aagagtcgtg gcaggatctg ccgcagaata
    15841 aactgactat ccgtctgcaa cctgatgaag gcgtggatat ccaggtactg aataaagttc
    15901 ctggccttga ccacaaacat aacctgcaaa tcaccaagct ggatctgagc tattcagaaa
    15961 cctttaatca gacgcatctg gcggatgcct atgaacgttt gctgctggaa accatgcgtg
    16021 gtattcaggc actgtttgta cgtcgcgacg aagtggaaga agcctggaaa tgggtagact
    16081 ccattactga ggcgtgggcg atggacaatg atgcgccgaa accgtatcag gccggaacct
    16141 ggggacccgt tgcctcggtg gcgatgatta cccgtgatgg tcgttcctgg aatgagtttg
    16201 agtaatatct gtgcttatcc tttatggtta ttttaccggt aacatgatct tgcacagatt
    16261 gtagaacaat ttttacactt tcaggcctcg tgcggattca cccacgaggc tttttttatt
    16321 acactgactg aaacgttttt gccctatgag ctccggttac aggcgtttca ctcataaatc
    16381 ctctgaatga aacgcgttgt gaatcatcct gctctgacaa ctcaatttca ggagccttta
    16441 tgaatccaca attgttacgc gtaacaaatc gaatcattga acgttcgcgc gagactcgct
    16501 ctgcttatct cgcccggata gaacaagcga aaacgtcgac cgttcatcgt tcgcagttgg
    16561 catgcggtaa cctggcacac ggtttcgctg cctgccagcc agaagacaaa gcctctttga
    16621 aaagcatgtt gcgtaacaat atcgccatca tcacctccta taacgacatg ctctccgcgc
    16681 accagcctta tgaacactat ccagaaatca ttcgtaaagc cctgcatgaa gcgaatgcgg
    16741 ttggtcaggt tgcgggcggt gttccggcga tgtgtgatgg tgtcacccag gggcaggatg
    16801 gaatggaatt gtcgctgcta agccgcgaag tgatagcgat gtctgcggcg gtggggctgt
    16861 cccataacat gtttgatggt gctctgttcc tcggtgtgtg cgacaagatt gtcccgggtc
    16921 tgacgatggc agccctgtcg tttggtcatt tgcctgcggt gtttgtgccg tctggaccga
    16981 tggcaagcgg tttgccaaat aaagaaaaag tgcgtattcg ccagctttat gccgaaggta
    17041 aagtggaccg catggcctta ctggagtcag aagccgcgtc ttaccatgcg ccgggaacat
    17101 gtactttcta cggtactgcc aacaccaacc agatggtggt ggaatttatg gggatgcagt
    17161 tgccaggctc ttcttttgtt catccggatt ctccgctgcg cgatgctttg accgctgccg
    17221 ctgcgcgtca ggttacacgc atgaccggta atggtaatga atggatgccg atcggtaaga
    17281 tgatcgatga gaaagtggtg gtgaacggta tcgttgcact gctggcgacc ggtggttcca
    17341 ctaaccacac catgcacctg gtggcgatgg cgcgcgcggc cggtattcag attaactggg
    17401 atgacttctc tgacctttct gatgttgtac cgctgatggc acgtctctac ccgaacggtc
    17461 cggccgatat taaccacttc caggcggcag gtggcgtacc ggttctggtg cgtgaactgc
    17521 tcaaagcagg cctgctgcat gaagatgtca atacggtggc aggctttggt ctgtctcgtt
    17581 atacccttga accatggctg aataatggtg aactggactg gcgggaaggg gcggaaaaat
    17641 cactcgacag caatgtgatc gcttccttcg aacaaccttt ctctcatcat ggtgggacaa
    17701 aagtgttaag cggtaacctg ggccgtgcgg ttatgaaaac ctctgccgtg ccggttgaga
    17761 accaggtgat tgaagcgcca gcggttgttt ttgaaagcca gcatgacgtt atgccggcct
    17821 ttgaagcggg tttgctggac cgcgattgtg tcgttgttgt ccgtcatcag gggccaaaag
    17881 cgaacggaat gccagaatta cataaactca tgccgccact tggtgtatta ttggaccggt
    17941 gtttcaaaat tgcgttagtt accgatggac gactctccgg cgcttcaggt aaagtgccgt
    18001 cagctatcca cgtaacacca gaagcctacg atggcgggct gctggcaaaa gtgcgcgacg
    18061 gggacatcat tcgtgtgaat ggacagacag gcgaactgac gctgctggta gacgaagcgg
    18121 aactggctgc tcgcgaaccg cacattcctg acctgagcgc gtcacgcgtg ggaacaggac
    18181 gtgaattatt cagcgccttg cgtgaaaaac tgtccggtgc cgaacagggc gcaacctgta
    18241 tcacttttta agacgacaaa tttgtaatca ggcgagagaa aactctgatg aaaaactgga
    18301 aaacaagtgc agaatcaatc ctgaccaccg gcccggttgt accggttatc gtggtaaaaa
    18361 aactggaaca cgcggtgccg atggcaaaag cgttggttgc tggtggggtg cgcgttctgg
    18421 aagtgactct gcgtaccgag tgtgcagttg acgctatccg tgctatcgcc aaagaagtgc
    18481 ctgaagcgat tgtgggtgcc ggtacggtgc tgaatccaca gcagctggca gaagtcactg
    18541 aagcgggtgc acagttcgca attagcccgg gtctgaccga gccgctgctg aaagctgcta
    18601 ccgaagggac tattcctctg attccgggga tcagcactgt ttccgaactg atgctgggta
    18661 tggactacgg tttgaaagag ttcaaattct tcccggctga agctaacggc ggcgtgaaag
    18721 ccctgcaggc gatcgcgggt ccgttctccc aggtccgttt ctgcccgacg ggtggtattt
    18781 ctccggctaa ctaccgtgac tacctggcac tgaaaagcgt gctgtgcatc ggtggttcct
    18841 ggctggttcc ggcagatgcg ctggaagcgg gcgattacga ccgcattact aagctggcgc
    18901 gtgaagctgt agaaggcgct aagctgtaaa agtcaaaatg cccgatctag gatcgggcat
    18961 ttttgtagcg tttttttgcc gggtttaacc ctgtactttt acctgtccgg cggcgtgctt
    19021 cgcgcgttca atggcgtcaa caacactctc tgaagtagcc agtaccaccc ccagacgacg
    19081 gctgccatca atttccggct taccaaataa acgaatctgc aaatctgcgc ctacggcatt
    19141 ctgcacatta tcaaacgtga cattctgact ggtcagttgt ggcagaataa cggcagaagc
    19201 tgcaggacca tactgacgga tcccgccaac aggaagtccg aggaaggcac gtacatgcag
    19261 ggcaaactct gagagatctt gagaaattaa cgtcaccatc ccggtatcat gtggacgagg
    19321 ggagacctca ctgaaaatca cctcatcacc acagacaaac agctcgacac caaacagacc
    19381 ataaccgccc agtgccagca ccactttacg ggcaatctcc tgcgcacgtt caagggcaag
    19441 cgggctcatt tgctgtggtt gccaggattc acggtagtcg ccatcttcct ggcgatgacc
    19501 tactggtgca cagaaatgga cgccatccac cgcgctgacg gttagcaggg taatttcgaa
    19561 gtcaaactta acgacgcctt caacaattac gcggcccgct ccggcgcgac cgccttgctg
    19621 agcgtattcc catgcctgag caagttgctc tgcagagcga ataaacgtct gccccttgcc
    19681 ggaagagctc atcaccggtt ttacaatgca gggatagcca atggcggcaa ccgcctcgcg
    19741 gaaaaggctt tcgctatcgg caaaacgata agtggaagtg ggcagctgca gctcttctgc
    19801 cgccaggcga cggataccct cgcgattcat cgttaatttc gttgcgcgag cgcaggggac
    19861 aacattcagt ccttcctctt caagttggat cagcatatcg gtggcaatag cttcgatctc
    19921 cggcacgata taatgtggtt tttccagttc aaccacacgg cgtaatgcat caccatcaag
    19981 catattaatg acatgggagc gatgcgcaac atgcatggct ggtgcgtcgg catagcgatc
    20041 gacggcaatc acctctacgc cgagacgctg acactcgatt gccacttctt tacccagttc
    20101 accggagcct aataacatca cgcgagttgc tgccggacgc agcgcagtgc ctaataacgt
    20161 cataacgaat atcccctgat taattccgcg cgcagtataa acgaaaacgt ttgcgtgtgt
    20221 ctttattgct gatgttgatt tctcaaccga aaagaaatat actgtataaa atcacagtta
    20281 ttatgagagg ctgcaaatgg cggttgaagt taaatacgta gtcattcgtg agggcgaaga
    20341 gaaaatgtcg tttaccagca aaaaggaagc cgatgcttat gacaaaatgc ttgatacggc
    20401 ggatctgctt gacacctggc tgacaaattc tccagtgcaa atggaagacg agcaacgtga
    20461 agccctttcg ctatggctgg cagaacaaaa agatgtgctg agcaccattc tgaaaaccgg
    20521 caaattaccg tccccacagg tcgtcggtgc tgaatccgaa gaggaagatg cctcacacgc
    20581 ggcctgaagc aacttgcgct cctgacaatt tgtaccatgc tttctcctga atcattgtgc
    20641 tatggagaaa aacatgaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc
    20701 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa
    20761 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    20821 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt
    20881 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa
    20941 agtgccgata ttcattacca ggtcagcgtg gactgcaaag cgggaatggc ggaatatcag
    21001 cggcgttaat ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    21061 tccgccccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    21121 aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat
    21181 cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc
    21241 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg
    21301 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    21361 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    21421 aattgttagt gccaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag
    21481 cacgtaaact tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag
    21541 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    21601 cgcagtttgg cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    21661 tggtctttgc cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc
    21721 agcaattgcg tgaagccggc gtggaagagc aggggcgtgt actcattgag caggtaatcg
    21781 aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg
    21841 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    21901 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    21961 atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt
    22021 tgactatttc gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct
    22081 gcataacatt cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt
    22141 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttcaaccag aaagaacaat
    22201 aacatgctac caaaagccgc ccgcattccc cacgccatga cgcttcatgg cgatacgcgc
    22261 atcgataatt actactggct gcgggacgat acgcgttctc agccagaagt cctggactac
    22321 ctgcaacaag aaaatagtta cggtcatcgg gtgatggcct cacaacaagc cttgcaggat
    22381 cgcatcttaa aggaaatcat cgaccgcatt ccgcaaagag aagtttctgc gccctacata
    22441 aaaaatggct accgctatcg gcatatttat gaaccaggct gtgaatatgc tatctaccag
    22501 cgtcaatcgg cattcagcga agagtgggat gagtgggaaa cattgctcga tgccaacaag
    22561 cgcgcagctc atagtgagtt ttattcgatg ggcggaatgg cgattacgcc cgataacacc
    22621 attatggcgc tggcagaaga ttttctttcc cgacgccagt acggcattcg ttttcgtaat
    22681 ctggaaacag gtaactggta cccggaactg ctggataacg ttgaacccag ctttgtctgg
    22741 gcaaatgact cctggacttt ctactatgtt cgcaagcatc cggtgacgct gctgccttat
    22801 caggtctggc gtcacgccat cggtacgcca gcatcgcaag atacactgat ctacgaagaa
    22861 aaagacgata cctattatgt cagcctgcat aaaacgacgt cgaagcacta tgtcgtcatt
    22921 catctggcca gcgccaccac cagtgaagtt cgcctgctgg acgcggaaat ggccgatgcc
    22981 gagccgtttg tttttctgcc gcgccgcaaa gatcacgaat acagccttga tcactaccag
    23041 catcgttttt atctgcgttc caatcgccac ggcaaaaact ttggcttata ccgtacccgt
    23101 atgcgtgatg agcaacagtg ggaagagtta attccgccac gcgaaaacat catgctggaa
    23161 gggtttacgc tgtttaccga ctggctggtg gttgaagagc gtcagcgcgg gttaaccagt
    23221 ttgcgccaaa ttaaccgcaa gacccgggaa gtcattggca ttgcctttga tgatccggcc
    23281 tatgtgacct ggattgccta caatccagaa cctgaaaccg cgcgattgcg ttatggttat
    23341 tcttccatga ccacaccaga cactttgttt gaactggata tggataccgg tgagcgtcgt
    23401 gtattaaaac aaacagaagt gcccggtttt gatgcggcga attaccgcag tgaacacctg
    23461 tggatagtcg cccgtgatgg cgtcgaagtt ccggtttcgc tggtctatca tcgcaaacat
    23521 tttcgcaaag gacacaaccc gttgctggtg tatggctatg gttcttacgg cgcaagtatt
    23581 gatgccgatt tcagttttag ccgcttgagt ttgttagatc gtggctttgt ctacgccatt
    23641 gtccatgttc gcggcggtgg tgagctgggg caacaatggt acgaagacgg taaatttctg
    23701 aagaagaaaa atacgtttaa tgattatctt gatgcctgcg atgcattgtt aaaactgggc
    23761 tatggctctc cttcgctctg ttatgcgatg ggcggaagtg cggggggcat gttgatgggc
    23821 gttgcaatta atcaacgccc ggaattattc cacggcgtta tcgcccaggt accgtttgtt
    23881 gatgttgtaa caacgatgct tgatgaatca attcctctta ccactggtga gtttgaagag
    23941 tgggggaatc cgcagggtcc gcaatattac gagtacatga aaagctacag cccgtatgac
    24001 aacgtcaccg cacaggctta tccgcattta ctggtaacga ccggtttaca cgattctcag
    24061 gtgcaatatt gggaaccggc aaaatgggtc gctaaattgc gcgagctgaa aaccgatgac
    24121 catcttttat tgctctgtac cgacatggac tcaggccatg gcggtaaatc tggccgcttt
    24181 aaatcgtacg aaggcgtagc gatggaatat gcttttctgg tcgcgctggc gcagggaaca
    24241 ttacccgcta cgcctgcgga ttaagtattt tccagataat gtttcagtgt taaacgcagc
    24301 tccgggctca tgctgtccag gttattaaat aaccagcgca gatagcccgg atcgcgttcg
    24361 gcaacgtcgg aaaccgcttt gccacggtat ttgccaaagg tgaaggttgt catcaacgac
    24421 ggacgtccgg tgatatcggc catctgttct gccgtccagc cggaggtgtt cataatatcg
    24481 ataagcaacg cggcggtgat ataacagtca tacaacgcgc ggtgatgatg caggcccggc
    24541 ggtgtctgta cattgagctt gcgtgtttta tataacgcca tattgctgta cttgagcctt
    24601 ggccacaaac gacgggccag tttcatagtg caaatccact caccgggcat ctcaggcagt
    24661 acgcggcggt caaagctggc gttgtgcgcg acataccatt cactaccgta atagtgtggg
    24721 atcacatctt caatccacgg tttatcggcg accatggctt cggtgatgcg atgaatcgcc
    24781 atcgcttgtg gactaatagg acgatcgggg cgcaccagga ggctcatggg gttgacgatt
    24841 tttccgtcaa tgacatcaac agaggcaatc tcaacgatcc ctccctgcaa accgcaggtt
    24901 tctgtatcga taatgcgcaa catggcatgc tccaggccga aaaagcctag cgtaatggaa
    24961 tgatatcgcc ttgccaaccc tgtgatgaac gctgaccgac taacaataat gaaccacgat
    25021 cggcgcgaac gatgagacga ccatattcat cccatagtgc actgttacca cgggcatttg
    25081 ccatcagcac ggcaatcgag tactgatgtg aaaaaaattg taagcgacgg gccgacgcca
    25141 gcaggtcggg ttctcccaga cattgccctg tcgtaaacag tgaacaggtc gggtccatat
    25201 ccataccttg cggttgttca tcgaccaccg tgatggttct ggaacgacgg cccagacatg
    25261 caccatgact ctgatggtag atcccaggcg tttttcgcca cggtgcgaac accgcaattc
    25321 caggaataaa gcgatcgtta tattcaacgg gaaggccggc aatgatggtc attcgccagg
    25381 tcgttgcggc ataacaaagc gggtctaaca gtgaaagatc gggtggggca ggaagggcac
    25441 gtcgcgaata atcacacccc agtaaagaaa gcgatggaaa aactaacagc tgacattgtt
    25501 gcctggcggc ggccctcaca aactccagat gatgagctac ctgttcggtg agtgaggttt
    25561 tacgaggctc atattgcgct gctgcaactt tccagaacga cataatgact tccttttcaa
    25621 aatgactgca aattatgggg gcggtttaga aaatactcat aatttagcag gcgttatgta
    25681 agaaagtgta actctgtata agttttattt aagtttgggc tcgtaaggta aacgtgacag
    25741 attgaccgaa gccaaacggt gggcaataag ccgctcgcga aaccagctgc gcaaatgttc
    25801 tggctgttca cgttcaaccg cttcagcgat caccggcata ttgtagcgtt ctttaaaagc
    25861 caccccggcc gccgccaaat cgacattcac tttatccatt tctgtttgat ccagtttagc
    25921 cagattcttc agcattctta atctcctcat cattcgcggg caaaagttac taagagtgag
    25981 cctggatggc aagccggaat tgatgatcct cgactcagac attagacaag gttatgctct
    26041 gtaaaaaagt tattacaaaa aggaatagat aatatggctt caactgccgc tcccttcgct
    26101 acgcgctggc aatcctgacc acttcgttgg tgacaccttc cgtctgggca catgcgcatt
    26161 taacgcatca gtatcctgcg gcaaacgcgc aagtgacagc tgccccgcag gcaatcactt
    26221 taaacttctc ggaaggcgtt gaaacaggat tcagtggtgc aaaaatcacg gggccaaaaa
    26281 acgaaaatat taaaacatta ccagcgaagc ggaatgagca ggaccaaaag caactgattg
    26341 ttccactggc cgattcgctg aaacccggaa cctacaccgt tgactggcat gttgtttcgg
    26401 tggacgggca taaaacgaaa ggacactaca cctttagcgt gaaataaatc atgctggcgt
    26461 ttacctggat cgcgctgcga tttatccatt tcacctcgtt gatgctggtc tttggctttg
    26521 caatgtatgg tgcctggctg gcccccttaa tgattcgtcg cttgctgacg aagcgttctc
    26581 tgcgtttaca acagcacgct gccgtctgga gtttgatcag tgccactgca atgctcgccg
    26641 ttcagggcgg gttgatggga acgggatgga cagatgtatt ttcgccaaac atttggcagg
    26701 ctgttttgca aacgcagttt ggtggcgtct ggttatggca aattgttctc gccctcgtaa
    26761 cgttgatcgt cgcccttatg caaccgcgga atatgccacg tttgctgttt atgctcacta
    26821 ccgcgcaatt tattctgctg gcgggagtgg ggcatgcgac gctgaatgaa ggggtaacag
    26881 cgaaaatcca tcagactaat cacgcgattc acctgatttg tgcggcggcg tggtttgggg
    26941 gattactgcc ggtgctctgg tgtatgcaac tcatcaaagg ccgttggcga caccaggcta
    27001 ttcaggcgct gatgcgtttt tcctggtgcg ggcattttgc ggtgatcggc gtactggcaa
    27061 gcggcgtgct taatgcattg ttgattaccg gatttccccc cacgcttacg acttactggg
    27121 gccaactctt attgctcaaa gccatcctgg tgatgattat ggtggtcatt gcgctggcta
    27181 atcgttatgt tcttgtaccg cgcatgaggc aggatgaaga tcgtgcggca ccctggtttg
    27241 tgtggatgac taaactcgaa tgggcgatag gtgcggtagt gctggtgatc atcagcctgc
    27301 ttgcgactct cgaacctttt tgatggatta cgaaaatgat gaaaaaaagt attctggcgt
    27361 ttctgttact caccagttct gcagcggcgc tggcggctcc acaggtgata accgtgagtc
    27421 gttttgaagt gggtaaagac aaatgggcgt ttaatcgcga agaggtaatg ctgacttgcc
    27481 gaccgggtaa tgctttgtat gtcatcaacc caagtaccct cgtgcagtat cctttaaacg
    27541 atatcgcaca aaaggaagtt gccagtggga agaccaatgc ccaacccatt tcggtgattc
    27601 agattgatga tcctaacaat cccggcgaaa aaatgagtct ggcaccgttt atagaacgag
    27661 ctgaaaaact ctgttaatta cctaaaatag ccttttgatt tccaataaaa aaaccgcctc
    27721 agttctttca ccagaacggg cggtttttaa catttaagct gatgaccacc gcgcttttta
    27781 ttgaccattt tgcacgcaaa ctggaaaacc tggcgtcgtc atctattctt aaagggcaag
    27841 gcaactaagc ctgcattaat gccaactttt agcgcacggc tctctcccaa gagccatttc
    27901 cctggaccga atacaggaat cgtattcggt ctttttttaa ttgcatttga aatcaattag
    27961 ttgcaaatgt ctcccccgaa attccccgaa atttactcga atttctgtat tccggtcttt
    28021 ttggttatat cacaaccaaa atacatttaa caatccattt acgttaaaat cagagcagta
    28081 agtacgtttt ttctctctca tcaagataca tttttgttgt cttctccgat gtgtggccaa
    28141 gtagacgctg agcaaattct tctccacatg tttctttgta caatcgtcca gccaggcttc
    28201 tgatctcgtg aaaagttggt gggttttcac tgaactggat acctgtgaat tttctggctg
    28261 cgacaaattt ttttgtcagg ccatccgggt gaatgctgcc gtcagggctg ttttttctaa
    28321 tcccggcact gattagataa tctccccggc ttaccaggcg gcatcgttca actactgtac
    28381 caagccgtag accagcgaca ggaaggctga gtgacagggg gatagcaatc atcattcctg
    28441 tcttaatttg cctgacgtgg agacgatcat cataaatatc actgaaccgc atattcgtta
    28501 tgtcttcgcg acgttgtcct gttacaaggg ctaaatccat agctaatggg aaccatgccg
    28561 gaagttgatc tgctgcctcc ctgatgcagt tgtatgtctt tagtttcagt cgttctcttg
    28621 taactactat tttcggtgct cttgttggcg tgactggatt ttgagatata cgtccttcaa
    28681 caatggcctc gcgaaacata tcagacaaca cagaacgcat tgatcctgcc attgtgtttt
    28741 tccctccttc aatccacaaa tcaagaaact cggcaatatg gcgagtggtt atttctgtca
    28801 gtaaaatctc tcccaatttt tcttttattg tctccagttg atttgcccga attttataag
    28861 tatttctgga cacttttctc cttataagaa tcgttttgta acgttcaatc cagtctgcca
    28921 tagtaaatga gtcgaaccct ttaagttttt caattaaggc agcgggagag tagtttttgt
    28981 atatataatg atttgcttca attgcctgcg ttactgcatc tctt
//
LOCUS       DBKEKI010000044        28936 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.044, whole genome shotgun sequence.
ACCESSION   DBKEKI010000044 DBKEKI010000000
VERSION     DBKEKI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.044"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..204)
                     /locus_tag="ACUQPX_001873"
     CDS             complement(<1..204)
                     /locus_tag="ACUQPX_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HHV9056599.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLM"
     gene            complement(241..1938)
                     /gene="dsbD"
                     /locus_tag="ACUQPX_001874"
     CDS             complement(241..1938)
                     /gene="dsbD"
                     /locus_tag="ACUQPX_001874"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HHV9056600.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(1914..2252)
                     /gene="cutA"
                     /locus_tag="ACUQPX_001875"
     CDS             complement(1914..2252)
                     /gene="cutA"
                     /locus_tag="ACUQPX_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HHV9056601.1"
                     /translation="MLDEKSSNTASVVVLCTAPYEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(2368..3669)
                     /gene="dcuA"
                     /locus_tag="ACUQPX_001876"
     CDS             complement(2368..3669)
                     /gene="dcuA"
                     /locus_tag="ACUQPX_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HHV9056602.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(3787..5223)
                     /gene="aspA"
                     /locus_tag="ACUQPX_001877"
     CDS             complement(3787..5223)
                     /gene="aspA"
                     /locus_tag="ACUQPX_001877"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HHV9056603.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSVGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            5560..6036
                     /locus_tag="ACUQPX_001878"
     CDS             5560..6036
                     /locus_tag="ACUQPX_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HHV9056604.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(6052..7308)
                     /gene="yjeH"
                     /locus_tag="ACUQPX_001879"
     CDS             complement(6052..7308)
                     /gene="yjeH"
                     /locus_tag="ACUQPX_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HHV9056605.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            7584..7877
                     /locus_tag="ACUQPX_001880"
     CDS             7584..7877
                     /locus_tag="ACUQPX_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HHV9056606.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            7921..9567
                     /gene="groL"
                     /locus_tag="ACUQPX_001881"
     CDS             7921..9567
                     /gene="groL"
                     /locus_tag="ACUQPX_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HHV9056607.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            9705..10058
                     /locus_tag="ACUQPX_001882"
     CDS             9705..10058
                     /locus_tag="ACUQPX_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HHV9056608.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(10261..11130)
                     /locus_tag="ACUQPX_001883"
     CDS             complement(10261..11130)
                     /locus_tag="ACUQPX_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HHV9056609.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(11525..12553)
                     /gene="epmB"
                     /locus_tag="ACUQPX_001884"
     CDS             complement(11525..12553)
                     /gene="epmB"
                     /locus_tag="ACUQPX_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HHV9056610.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            12595..13161
                     /gene="efp"
                     /locus_tag="ACUQPX_001885"
     CDS             12595..13161
                     /gene="efp"
                     /locus_tag="ACUQPX_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HHV9056611.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            13213..13338
                     /gene="ecnA"
                     /locus_tag="ACUQPX_001886"
     CDS             13213..13338
                     /gene="ecnA"
                     /locus_tag="ACUQPX_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HHV9056612.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            13449..13595
                     /gene="ecnB"
                     /locus_tag="ACUQPX_001887"
     CDS             13449..13595
                     /gene="ecnB"
                     /locus_tag="ACUQPX_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HHV9056613.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            13771..14088
                     /gene="sugE"
                     /locus_tag="ACUQPX_001888"
     CDS             13771..14088
                     /gene="sugE"
                     /locus_tag="ACUQPX_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HHV9056614.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(14085..14618)
                     /gene="blc"
                     /locus_tag="ACUQPX_001889"
     CDS             complement(14085..14618)
                     /gene="blc"
                     /locus_tag="ACUQPX_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HHV9056615.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14707..15840)
                     /locus_tag="ACUQPX_001890"
     CDS             complement(14707..15840)
                     /locus_tag="ACUQPX_001890"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HHV9056616.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            complement(15903..16262)
                     /gene="frdD"
                     /locus_tag="ACUQPX_001891"
     CDS             complement(15903..16262)
                     /gene="frdD"
                     /locus_tag="ACUQPX_001891"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HHV9056617.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(16273..16668)
                     /gene="frdC"
                     /locus_tag="ACUQPX_001892"
     CDS             complement(16273..16668)
                     /gene="frdC"
                     /locus_tag="ACUQPX_001892"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HHV9056618.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(16679..17413)
                     /gene="frdB"
                     /locus_tag="ACUQPX_001893"
     CDS             complement(16679..17413)
                     /gene="frdB"
                     /locus_tag="ACUQPX_001893"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HHV9056619.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(17406..19214)
                     /gene="frdA"
                     /locus_tag="ACUQPX_001894"
     CDS             complement(17406..19214)
                     /gene="frdA"
                     /locus_tag="ACUQPX_001894"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HHV9056620.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            19539..20516
                     /gene="epmA"
                     /locus_tag="ACUQPX_001895"
     CDS             19539..20516
                     /gene="epmA"
                     /locus_tag="ACUQPX_001895"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HHV9056621.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     LLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            20735..22237
                     /gene="yjeM"
                     /locus_tag="ACUQPX_001896"
     CDS             20735..22237
                     /gene="yjeM"
                     /locus_tag="ACUQPX_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HHV9056622.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDMNFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMLSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(22388..25699)
                     /gene="mscM"
                     /locus_tag="ACUQPX_001897"
     CDS             complement(22388..25699)
                     /gene="mscM"
                     /locus_tag="ACUQPX_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HHV9056623.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMSQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPML
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICCALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(25727..26689)
                     /gene="asd"
                     /locus_tag="ACUQPX_001898"
     CDS             complement(25727..26689)
                     /gene="asd"
                     /locus_tag="ACUQPX_001898"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934919.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HHV9056624.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLACNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGITKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKD"
     gene            complement(26786..27838)
                     /gene="rsgA"
                     /locus_tag="ACUQPX_001899"
     CDS             complement(26786..27838)
                     /gene="rsgA"
                     /locus_tag="ACUQPX_001899"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HHV9056625.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            27933..28478
                     /gene="orn"
                     /locus_tag="ACUQPX_001900"
     CDS             27933..28478
                     /gene="orn"
                     /locus_tag="ACUQPX_001900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HHV9056626.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            28689..28764
                     /locus_tag="ACUQPX_001901"
     tRNA            28689..28764
                     /locus_tag="ACUQPX_001901"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28722..28724,aa:Gly,seq:gcc)
BASE COUNT         6994 a         7296 c         7556 g         7090 t
ORIGIN      
        1 catcagctga cgacgccaga catctatctg ttggctaaga taacgcagcg catcgtagag
       61 gattgcctct ttgtctggcc agaagcggcg tagctcgtcc agtggataat ccacacgttc
      121 agcaaccatc tccagcgtgg tgttggcaat cccttgtaat tctaataatt tcagggcttc
      181 tcccagtaca tcttcacgtt gcacgctatt ttcctccgtc tttcccactg caagtgtcgt
      241 tcacggttgg cgatcgcgca aatgtgcgct gaaggtttca gcatccataa agcccgtgac
      301 gcgtgcttgt ggatgctcct ggccttgtcc gtcaaaaaag agaattgtcg gtaggccaag
      361 gacattaaga tgctttaaca gcgccacatc ttgtgcgtcg ttggccgtga cgttggcctg
      421 aagtaagacc gtgtctgcta acgctttttg cacctgcggg tcgctgaagg tgtatttctc
      481 aaactcttta caggcgacgc accagtcggc ataaagatct aacatcaccg gtttgccttt
      541 ggcttcaacg agcgcctgat ttaactcatc taccgttttg atttgtgtaa agttgagatg
      601 cgtctgagtt tgcgcggtat gcgtcgcacc aaatgcccaa tcctgaagtg ggcgcacgct
      661 aaccaatgcc gctgccagca ggattatttg caccacgcgc atccagccgc gtttagcctg
      721 taggctggtg ataaaggccc agccaaagaa tgcgacaccc agcgccgacc acaagcgtaa
      781 tccccataca tcaccaatca ctcgctccag caggaagacc ggcagtgcga ggatcacaaa
      841 accaaacgcg gttttgactt gttccatcca cgggccgctt ttcggcagca agcggttacc
      901 aaagacggta attagcatca gcggcaggcc catgcccaac gcatagagat aaagcgtgcc
      961 gccgcccagc cacatgttcc cgctttgggc gatatacagc agaatcgcgc taagcggtgc
     1021 ggtggtgcat ggtgaacaga tcagtccggc aatcgccccc ataacaaaca caccgccagg
     1081 tgagccgccc tgttggcgat tgctcatcaa cgtgagacgt gtttgcagcg aagaggggag
     1141 ttgtagagta aacaagccaa acattgacat cgccagcaag gtaaagacga tggcgaggcc
     1201 aatgagcacg tatgggtgct gtagcgccgc ctggaactgt aaccctgcgg cggcaactac
     1261 cagacccagc gccgtgtaag tcagtgccat cccctgcaca taaataaagg tcagcaacaa
     1321 tgctctggca gtggagagcc gctgtttacc acccagcacg atgccagaaa tcagtgggta
     1381 cattggcagc acgcatggcg taaaggcgat accaataccg atcaacaacg cccagagcgc
     1441 ggaaaagggc aattgcgcgg tgtgctgctc ttgctgcgaa acagacacag gctgtgacgc
     1501 tgcgttgttg gcgaccactt cgcttaacgg aacggttttg gtttctggcg gataacagaa
     1561 accggcatca gcacagccct ggtaggtgac agttaacgtc gctcccgcac tcgcctggtt
     1621 gatggttacg ggaagcgtca gccgatcgcg gtaaatctcg cttttgccgt aaaactcatc
     1681 ttcatgccag acgccttgcg gcagctgcac gtcggcaatt ttcgcgtgtt ccggcgtaat
     1741 gcggatctgt ttacggtaga ggtagtaacc gtctttgatc tgccaggtca gattaaggtc
     1801 atgttggttt tgctgaaaat caaaagcaaa ggcttgatcc gcggggacaa attgtgaacg
     1861 tcccggcgcg tcgaataatc cggcaaaaac ggaagtgctg caaagtagca gaatcagcgt
     1921 aaagatgcgt tgagccatga gaggtaatct gtgtctccgt gtgtaacagg taaaaccaga
     1981 agttccgggg tttgatatgg atgatgagac ttcaggcatt ccagcagtgc ctgctggtga
     2041 gatacggtag tttttaaaat catctgcact tcgtattctt gctccagctt accttcccag
     2101 taatagagag aggtagcgcc ggggatcaag gtcgcgcagg ccgccagttt ttccgccagc
     2161 actttggcgg ctaaatcctg ggctgtcgct tcatacggtg cggtacatag caccacgaca
     2221 gacgcggtat tcgaactttt ttcatcaagc ataaacacct cgcaagaaca gatggaatcg
     2281 caagagagag gtcactatac aacggacggg ggaaggatgt tagtcggcgg gaaagaaagc
     2341 ggggcgtgaa cgccccgcga ttgatcatta cagcatgaag ctacccagca cgaagccgaa
     2401 gcaaacggcc agggcaacac ccagagtacc cgggatgaag aacggatggt tgaagacgaa
     2461 tttaccgata cgggtagtac ccgtgtcatc catctgtacc gcagcaacca gcgtcgggta
     2521 ggtcggcaga atgaacagac cagacaccgc agcgaaagaa gcaacagcgg tcagcggtga
     2581 aacgttcagt gccagagcca tcggcatcag tgcttttgcg gttgcagcct gagagtacag
     2641 cagagcagaa gcaaagaaga agatgacggc cagcagccac ggatgaccct gaatcacttc
     2701 accagcggta tctttgatcc agtcgatgtt gttggaaacg aaagtatcgc ccagccacgc
     2761 aacacccagg atacaaatac aggcgctcat acctgctttg aaggtgctgg agttgaggat
     2821 gttgtcggta tcgactttac agataacggt ggtcagagtt gcaacgctga gcatgatgat
     2881 caggattgcg ttggtggtgt tcatcagcgg tttttcaacc agacccatgc ttgggctgtt
     2941 gatgattgca tagataacca cgccaactac gcccagcagg aacagccaga cggacgtttt
     3001 tgcaccggat ttgatttcaa tctgcttttc accgcgcagt tcaaccaagc cctcttccag
     3061 acgcttgcga taaatcggat cgtcagagag tttggagttg aacagcatag tgaccaggaa
     3121 ggacatcacc agaaccgcca gcagggtgga cgggatgacc acggagagca gatggaggta
     3181 gctgatgcca tgaccttcca tcacggaaga catgtaaacc actgccgctg agattggcga
     3241 tgcggtgatc gcaatctgcg cggataccac tgcagtagag agcggacggc aaggtttaac
     3301 gccttgttcc ttcgcaactt cagcgataac tggcagtgtc gccagagaga tgttgccagt
     3361 accagcaaag atagtcagga aataggtcac gatcggtgcg aggatcgtga tgtatttcgg
     3421 gttacggcgc agcagctttt ctgtctgatg aaccagatag tccagaccgc cagcaacctg
     3481 catggcagaa atagcggcga taaccgccat gataatggaa atgacatcga acgggatgtt
     3541 accgggttta acgccaatag cggcaagaac cagcaccccc aatccgcctg caaaaccaat
     3601 acctattccc cccaatctgg cgcccaagaa gatcgccagc aaaactatga tgagttctac
     3661 aactagcata ttagccttcc ttgtttttta acaagttgat attagattgt tatttttaag
     3721 ttactgctca caagaaaaaa ggcacgtcat ctgacgtgcc ttttttattt gtactaccct
     3781 gtacgattac tgttcgcttt catcagtata gcgttttgct ttgtaagccg ggtgcatcag
     3841 attctgtacg gagaaaatat cgtcaagttc cgcttcagtc aacagaccgc gttccagaac
     3901 gacttcacgt acactcttac cggtttcggc acagatttta cccacgatgt caccgttgtg
     3961 gtgaccgatg aacgggttca ggtaagtaac gataccgaca gagttgtaaa cgtaaccttc
     4021 gcacacttct ttgttagcag tgatgccgtt aatgcatttt tccagcaggt tgtagcaagc
     4081 gttggtcaga atgtgaacgg attcgaacat ggcctggcca atgaccggtt ccataacgtt
     4141 caactgcagc tgacctgctt ctgctgccat ggtaacagtg gtgtcgttac cgatgacttt
     4201 gaagcatacc tggttaacca cttccggaac aaccgggttt actttagctg gcatgatgga
     4261 agagcccgcc tgcagttccg gcaggttgat ctcgttcagg ccggcacgtg gaccagaaga
     4321 gagcaagcgc aggtcgttac agattttgga catcttcaca gccaggcgtt tcagcgcgcc
     4381 gtgaaccata acataagcgc cgcagtcaga ggtcgcttcg atcaggtctt cagccggtac
     4441 gcatgggaag ccagtgactt cagccagttt tttcactgcc agcggagagt actctttcgg
     4501 cgtgttcaga ccagtaccga ttgctgttgc gccaaggtta acttccagca gcagttcagc
     4561 ggtacgttgg atgtttttca cttcttcttt cagcaggatg ctgaaagcgc ggaattcctg
     4621 accgagggtc atcggtactg cgtcctgcag ctgggtacga cccattttca ggatgtcctg
     4681 gaattcgaca gctttacgtt caaagccttc acgcagttgg ttaatcgcat ctaccagctt
     4741 aatcagggaa gagtaaactg cgatacggaa accggtcggg taggcgtcgt tagtggactg
     4801 acatttgtta acatggtcgt tcgggttcag gtactgatat tcacctttct ggtgacccat
     4861 cagttccaga ccgatattgg ccagcacttc gttggtgttc atgtttacgg aagtacctgc
     4921 gccgccctgg tagacgtcta ccgggaactg atccatgcat tttccgttgt tcaggacttc
     4981 atcacatgcg gcaatgatgg cattcgctac acttttagga atggtttgca gctctttgtt
     5041 tgccatagct gcggcttttt taaccattac cataccgcga acaaattcag gaatatcact
     5101 gattttgttg ttgctgatat agaagttttc aatcgctctc agagtgtgaa caccatagta
     5161 ggcatcagct ggaacttccc tggtacccaa cagatcttct tcgatacgaa tgttgtttga
     5221 catgtgaacc ttctttttca agctgccaat gatttgcttt aaacacacag aatatatgtg
     5281 gtttcgaatg tttttcgacc gacgattatc ccctgcatcg accgaatacc cgagatcata
     5341 tgctgcttga ggatttctac cgtaatctgg atcactttaa gtgtcggttt ttacccctta
     5401 attattaatt tgtgaaatag atcaccgctt tgggattact accaaaaata gttgcgcaaa
     5461 catcttgaaa ttttgctaat gaccacaata taagctgaac gcgattcgca acccattcag
     5521 gtagccgggt ttaaccggct gctattacag gagaaacctt tgcgctggtt accttttatt
     5581 gccattttcc tttatgtcta tattgagatt tcaatcttta ttcaggttgc ccatgtattg
     5641 ggggtattgc tgaccctcgt gctggttata ttcacgtcag ttatcggtat gtcactggta
     5701 cgtaaccagg gctttaagaa tttcgtgctg atgcagcaaa aaatggcggc gggtgaaaat
     5761 cctgcggcgg agatgattaa aagtgtttcg ctgattattg ccggtttgct gcttttatta
     5821 ccgggctttt tcaccgactt cctcggtctt ctgcttttat tgccgccggt gcaaaagcat
     5881 ctgacagtga agttgatgcc acatttgcgc ttttctcgca tgccaggcgg cggttttagc
     5941 gccgggaccg gtggcggtaa tacttttgat ggtgagtatc agcgaaagga tgatgaccgc
     6001 gaccgccttg atcataaaga cgatcgccag gattaatgtc gaaacgccgg attatgtggt
     6061 tatgccattt tccggcgttt ttcgttttgg cagaaacagc cataacccca ccagcatgat
     6121 cagcgcatag agacttttcc agccgaccat tgccagtaac agaacgcata acagcccgcc
     6181 aaccaccgcc agtagtcgat aacgaccttg caataattta cagcctgcca gcatgcataa
     6241 cagataaatc ataataaaga tgccattggc ataaataata agagcgtcca gattgatctc
     6301 taaggcatga attaccaacg tgctcaccac gcagcagcca agcaccgcat tgagggcatt
     6361 attcgggata tggcgagaag agaggcgtgc taggtagtgg tcaggattat gttgcgcctg
     6421 cgaccagacc aggcgggcga agctctgtat ataaatgtta agactggcaa agcaggccag
     6481 atagccaatt acgcaggcaa tccataacgc tcctacaccg aacaactgca ctacaatttt
     6541 tggaagcgat gctgccgccg ccatttgttc accataggcg tcgaagtgta agacgactac
     6601 cgtacagccc cagtagacta atcctgccag cagcagacca atcatcaaag cacgaggaaa
     6661 atcacgctct ggatttttaa attccgaggc aagatgggca aatgcttcca gaccgacaaa
     6721 acaccagaac atcactgata acgcagcaaa taacccggta agttcgatat ttcctggcgc
     6781 ggggaagggg atactcgcag gtttgatatc gcccgcccac cagatagcga caatcagtgc
     6841 gacgataagt ccggcaataa cggtttgtag attagcactg gaactggcac cgagagtacc
     6901 gatataccac accaacgcca gcgtaccgag ttctgccaac aacagttgcc agctatgcca
     6961 gccaaacatt gcctggccga atccggcggc aatttgtagt gcggcaggca aacccacggg
     7021 aatgaccgat aaaaacagcc agccggtgac tcgctcaagc cgcgaaccaa acgccatacc
     7081 gacgaagtgc gcgacgccgc ctgcgctggg atagtggcga cccagaatcg caaacacaat
     7141 cgcaatcggg aacactaaga taatcaaaac gggccacgcc cacaggctgt tattgcctgc
     7201 taccagtgca gctaacgcag gaacggcaaa cacgccagtg cctaataatg acgtcgatag
     7261 caggccaatg ccctgggcca gccccagttc ttgtttgagt ccactcatgg gttgatatcc
     7321 gattgcgccc aaattttggg caactgcgta gattttcgat ggtagcacaa tcagattcgc
     7381 ttatgacggc gatgaagaaa ttgtgatgaa atgtgaggtg aatcagggtt ttcacccgat
     7441 tttgtgctga tcagaatttt ttttcttttt cccccttgaa ggggcgaagc ctcatcccca
     7501 tttctctggt cacctgccgg gaaaccacgt aagctccggc gtcacccata acagatacgg
     7561 actttctcaa aggagagtta tcaatgaata ttcgtccatt gcatgatcgc gtgatcgtca
     7621 agcgtaaaga agttgaaact aaatctgctg gcggcatcgt tctgaccggc tctgcagcgg
     7681 ctaaatccac ccgcggcgaa gtgctggctg tcggcaatgg ccgtatcctt gaaaatggcg
     7741 aagtgaagcc gctggacgtg aaagttggcg acatcgttat tttcaacgat ggctacggtg
     7801 tgaaatccga gaagatcgac aatgaagaag tgttgatcat gtccgaaagc gacattctgg
     7861 caattgttga agcgtaatcc gcgcacgaca ctgaacatac gaatttaagg aataaagata
     7921 atggcagcta aagacgtaaa attcggtaac gacgctcgtg tgaaaatgct gcgcggcgta
     7981 aacgtactgg cagatgcagt gaaagttacc ctcggtccaa aaggccgtaa cgtagttctg
     8041 gataaatctt tcggtgcacc gaccatcacc aaagatggtg tttccgttgc tcgtgaaatc
     8101 gaactggaag acaagtttga aaatatgggt gcgcagatgg tgaaagaagt tgcctccaaa
     8161 gcgaacgacg ctgcaggcga cggtaccacc actgcaaccg tactggctca ggctatcatc
     8221 actgaaggtc tgaaagctgt tgctgcgggc atgaacccga tggacctgaa acgtggtatc
     8281 gacaaagcgg ttaccgctgc agttgaagaa ctgaaagcgc tgtccgtacc gtgctctgat
     8341 tctaaagcga ttgctcaggt tggtaccatc tccgctaact ccgacgaaac cgtaggtaaa
     8401 ctgatcgctg aagcgatgga caaagtcggt aaagaaggcg ttatcaccgt tgaagacggt
     8461 accggtctgc aggacgaact ggacgtggtt gaaggtatgc agttcgaccg tggctacctg
     8521 tctccttact tcatcaacaa gccggaaact ggcgcagtag aactggaaag cccgttcatc
     8581 ctgctggctg acaagaaaat ctccaacatc cgcgaaatgc tgccggttct ggaagctgtt
     8641 gccaaagcag gcaaaccgct gctgatcatc gctgaagatg ttgaaggcga agcgctggca
     8701 actctggttg ttaacaccat gcgtggcatc gtgaaagttg ctgcggttaa agctccgggc
     8761 ttcggcgatc gtcgtaaagc tatgctgcag gatatcgcaa ccctgactgg cggtaccgta
     8821 atctctgaag agatcggtat ggagctggaa aaagcaacct tggaagacct gggtcaggct
     8881 aaacgtgttg tgatcaacaa agacaccacc actatcatcg atggcgtggg tgaagaagct
     8941 gcaatccagg gccgtgttgc tcagatccgt cagcagattg aagaagcaac ttctgattac
     9001 gaccgtgaaa aactgcagga gcgcgtagcg aaactggcag gcggcgttgc agttatcaaa
     9061 gtaggtgctg ctaccgaagt tgaaatgaaa gagaaaaaag cacgcgttga agatgccctg
     9121 cacgcgaccc gtgctgcggt agaagaaggc gtggttgctg gtggtggtgt tgcgctgatc
     9181 cgcgtagcgt ctaaactggc tgacctgcgt ggtcagaacg aagaccagaa cgtgggtatc
     9241 aaagttgcac tgcgtgcaat ggaagctccg ctgcgtcaga tcgtcctgaa ctgcggcgaa
     9301 gaaccgtctg ttgttgctaa caccgttaaa ggcggcgacg gcaactacgg ttacaacgca
     9361 gcaaccgaag aatacggcaa catgatcgac atgggtatcc tggacccaac caaagtaacc
     9421 cgttctgctc tgcagtacgc agcttctgtg gctggcctga tgatcaccac cgaatgcatg
     9481 gttaccgacc tgccgaaaaa cgatgcagct gacttaggcg ctgctggcgg tatgggcggc
     9541 atgggtggca tgggcggcat gatgtaattg ccctgcactt cgcagaaata aacaaacccc
     9601 cgggcagaaa tgtctggggg tttttctttt ggtcatcttt ctagtataag attcagacac
     9661 ggacgacgcg agtggcgtcc agctcattga ttatggggaa taacatgcac gtaaaatact
     9721 tagcagggat tgtcggtgcc gcgctactga tggcgggttg tagctccagc aacgaattga
     9781 gtgctgccgg tcagagtgta cgcattgtgg acgagcagcc aggcgcagag tgccagctga
     9841 ttggtactgc gacaggtaag caaagcaact ggctttccgg gcaacacgga gaagagggcg
     9901 gttctatgcg cggcgcagca aacgatctgc gcaaccaggc ggctgcaatg ggcggtaacg
     9961 tgatttatgg catcagcagc ccgtcgcagg gaatgttgtc cagttttgtc ccgacggata
    10021 gccagattat cggtcaggtt tataagtgcc cgaactgatg tgatccagag gctgggtgac
    10081 aaacgtatta ttgcctgatg cgcttcgctt atcaggccta cagcaggcat tgcaactgat
    10141 tgaatgcttg agaatttgta ggccggataa ggcgtttacg ccgcatccgg catgaccaac
    10201 gatcacgtta tcagtcattt caggctggat ggctccagcc tcgcattagc aaagatgaga
    10261 ttatttcgcc tgtggtgcag ttttgctggt ggattgtaca cgtaagtgat gcagcgcgcg
    10321 taagcattga tcctgggtta gcggttgggc gttattcgcc gctacggtgc gaacatagac
    10381 ctgacatggc acacccgcga gttttttcag tcgtgggctg aaatcaagca ttcggcgagt
    10441 gatcgcctct atttctttac ccggcgttgc ttcacgcgtt ttcaccactg cgccgctaaa
    10501 ttgttctttg ccactttcat ctttaaagcg gtaaagcacc gccagataat ggtcgaacaa
    10561 gttatgtttc cattccggtt gggaataggt gtcatattcc agattgccat tgtcctggca
    10621 gtcaacgtcg tataacggca ggaaatctaa cagtgaggta atacgtaatg ccagttcacg
    10681 catttccgca ttattaattg catgaatgat tgctcgcgcc agcatttcaa tctgcaactc
    10741 attgacgact aactcacatt tgctaccgtc atgtagcgtt aatacgaagg taagattttc
    10801 gccttgatta tcggtaagtt ccagcgtgtt aacccgacga ttaatatcgg catttttaag
    10861 ctcatcaacc agaatttctg ggatgttttc tgccattttc ttaatgactt tatcgcgagc
    10921 ttgctcatac tgaaggcgtg cagcagcatc cagtttatgc ttttgatgca gacgactttc
    10981 cagcgcaatc agcaggtcgc gcagttccat gacagacata aagaacaacg attctttatt
    11041 gcgaggttct ttaattttca gcgccagtgc aatgaaatta ttactcttgc gaataacacc
    11101 ggtattaaca cccttgatgc ttatggccat gtaggttctc cctaaccatt tctcaattaa
    11161 ataattaatt ttaatttata agccagataa atgggcttgg tagtaatagt tgttaaaata
    11221 acataaatag ccgtgctcac tctatataaa cagaaaagaa aggttaatta ttggtgttag
    11281 ctatataaaa agtaacaaca gcaatgcata tgaataatta aaggttatgc aacgtgcaaa
    11341 gattaaattt ctcagtttaa tgacaggttg ctgtacttgc gtttgttgct cttgccggat
    11401 gcggcgtaaa cgccttatcc ggcctacagt tcattgcaaa tccattcaat ttcaatgcac
    11461 gatgtaggcc tgataagcgt agcgcatcag gcaattttaa tgtttaactt ccctgtttaa
    11521 tcagttactg ctggcgtagc tggagatcca gcggcgtttt gctgggttcg ccgccaattt
    11581 ctcgcgccag tttcggcacc agatatcccg acaccaatgt cagcaactca cgcataatct
    11641 gccgtgcttc gtcatcactc accataaaat gcgccgcgcc ctgtacttta tctagcacat
    11701 gcaggtaata cggcatcacg ccggcatcga acaacgcatt actcaggttt gccaacgttt
    11761 gtgcgttatc gttcacacca cgtaacagaa cgctctggtt aagcagggtg acaccgacac
    11821 ggcgcaattt agccatcgcc tgacggaacg tttcatctac ctcattggca tgattgatgt
    11881 gattcaccag caagatttgc agcgtagaac gggcaaagcg ttcaaccagc gcctcggtga
    11941 tacgtgccgg gatcacaatc ggcagacggc tgtgaatccg cagacgtttg atatgcggga
    12001 tggcttccag ttgtgtgagc aaccagtcca gctcgtgatc tttcgccatc agcggatcgc
    12061 cgccggagaa aatcatctcg tccagttccg gatgcgcagc aacatactca agtgcagtct
    12121 gccagttacg cttgttgccc tgattttcgg cataggggaa gtggcgacgg aagcaatagc
    12181 ggcaatttac cgcgcagccg cctttgacca gcaaaagcgc ccggttgtgg tatttatgca
    12241 acaaaccagg cactacgctg tgctgttctt ccagtgggtc ggtggagaat ccggacgcga
    12301 cgacaaactc atcttgcgag gtaagtacct gacgcaaaag aggatcgtcc ggattgcctt
    12361 tctccatgcg atcgataaat gagcggggca cacgcagggc aaacagcttt ttggcgctgc
    12421 gtccggccaa cagtttttcg tccgcgtcta tattcaaaag acgcagaagt tcatcaggat
    12481 cggtcacaac atcggcaagt tgcgttaacc aatcttctct ggatggggta tttagggtta
    12541 caatatgcgc cattttgtgg cttagctacc aattaacaaa tttcagaggg ccttatggca
    12601 acgtactata gcaacgattt tcgtgctggt cttaaaatca tgttagacgg cgaaccttac
    12661 gcggttgaag cgagtgaatt cgtaaaaccg ggtaaaggcc aggcatttgc tcgcgttaaa
    12721 ctgcgtcgtc tgctgaccgg tactcgcgta gaaaaaacct tcaaatctac tgattccgct
    12781 gaaggcgctg atgttgtcga tatgaacctg acttacctgt acaacgacgg tgagttctgg
    12841 cacttcatga acaacgaaac tttcgagcag ctgtctgctg atgcaaaagc aattggcgac
    12901 aacgctaaat ggctgctgga tcaggcagag tgtatcgtaa ctctgtggaa tggtcagccg
    12961 atctccgtta ctccgccgaa cttcgttgaa ctggaaatcg ttgataccga tccgggcctg
    13021 aaaggtgata ccgcaggtac tggcggcaaa ccggctaccc tgtctactgg cgctgtggtt
    13081 aaagttccgc tgttcgtaca aatcggcgaa gtcatcaaag tggatacccg ctctggtgaa
    13141 tacgtctctc gcgtgaagta atgcggttgt ggtgcggcct gcaggctgca ccatcactta
    13201 ttcaggtcag agatgatgaa acgccttatc gttcttgttt tgcttgccag cacgctgctc
    13261 acgggctgta acaccgcttg cggtttcggc gaagacatca aacatctcgg caactccatc
    13321 tctcgcgctg ccagctaatt tttcttctct tccgaaaaat catcagattt ccatcatttt
    13381 tggcgatgtt gtctattatt aatttgctat aggcaaacat aaataacatt acctaaaagg
    13441 aagacgttat ggtgaagaag acaattgcag cgatcttttc tgttctggtg ctttcaacag
    13501 tattaactgc ctgcaacacc acgcgtggcg ttggtgaaga catttctgat ggcggtaacg
    13561 cgatttctgg cgcagcaacg aaagcgcagc aataagcaat aacggtacga cagcagtgtc
    13621 gtgccgtttg ttttttctgc gatcgtcata aaggtaatag ttgaaattcc cctgccacct
    13681 ggcaaaatat ccgttcaacc atcagctttg caggacgacc tgcaaacgcc tcttttcacc
    13741 ggggacggcc ccaaatttcc ggagcctgat atgtcctgga ttatcttagt tattgctggt
    13801 ctgctggaag tggtatgggc cgttggcctg aaatataccc acggctttag ccgtttgacg
    13861 ccgagtgtta ttaccgtgac ggcgatgata gtcagtatgg cgctacttgc ctgggcgatg
    13921 aaatcgttac cggtagggac ggcttatgcc gtgtggacgg gtattggcgc agtcggcgcg
    13981 gccatcaccg gcattgtgct gctcggtgag tccgctaacc cgatgcgtct ggctagtctg
    14041 gcgttaatcg tattggggat tattggtctg aaactcagca ctcactaact accgggctgc
    14101 tgtacccaaa taaacttact gacatcaaac ccttcccggg tcgcgactgc cagcatctcc
    14161 tgtttcactt cgtcagaaat ggttggcgtg cgggagagta tccacaggta gtcgcggtcc
    14221 gggccgcaaa ccagcgcatg gcggtattcc cgatcgagtg caataacgtt ataaccgcca
    14281 tagaaaggac caaagaatga cactttcagc gcagcgcgag ttggtgcgcc ggtaaagtac
    14341 gctttccctt cactctgctg ccacattcct ctgtcagggt tatagccttt attaatgaca
    14401 ttcaggccgc cgtcatcacg caggctgtat gttgcggtga ctttttccag tccacgttca
    14461 aaacggtgat caaaacgggc aatctcatac caggtaccga gataacgttt ggcgtcgaaa
    14521 ttattcacta cggtcacgcc acgcggcggc gtaggagaac tacaggcaac gaccagaaat
    14581 gcagctgtcg ctgcggcaac gagagggagc aggcgcataa atgtttcttt actggttttt
    14641 ttctaagtgt agatgacagc aaggaaaagc gcagaaaagg tccgaaaatt cggacccgat
    14701 gaaattttac tgtagagcgt tgagaatctg acaggcggcg gcgactctcg ctggattggg
    14761 atagtttttg ttagccagca tcacgatacc cagctctttt tctggaataa acgcgacata
    14821 gctaccaaat ccgccggtcg cccccgtttt atgtacccat gatgcgcgta ctgcaggagt
    14881 tgggggctta atcgctttta ccgggcgtgc tgccagtgca attttattgt cactgccgtt
    14941 aatgatgctg tcaggattga ccggccagtc cagcatttcc cagcccaggc cctgatacat
    15001 atcgccggtt tgccagtagc gagattgtgc cagttgtatc ccttgttgaa gcgttttctc
    15061 attgatatca aggggggtta aattgctttg cacccagcgg gccatatctt caatggtcga
    15121 cttcacacca taagcttcag catctaacgc ccctggcgaa acatgcactg ccttaccttc
    15181 gcgatatccc caggcgtaat tcttttcttc tgggggaggt acattaatcc acgtatggtt
    15241 gagtttgagt ggctggaaga cacgagtttg catcgcctgc tcaaaactca aaccagacgg
    15301 cttcacagcc agtgcgccga acaaaccgat actggagttg gcatacagac gttgtgttcc
    15361 tggagcccat gcaggctgcc agttttgata gaagcgcagc aagtcgcttg aggatttcac
    15421 ttcatccggc acctgcaatg gcaggccgcc agcggtgtag gtcgcaagat gtaatagtgt
    15481 gatcccattc cactgtttag cggtaagttc aggccagtat tttgttgcgg gatcgcttaa
    15541 cttgatttcc cctcgagcaa tagcgtcgcc accaagcacg cccgtaaatg ttttgctgac
    15601 cgaacctaac tcaaacaacg tttgctgtgt gacgggctgc tttttggcga tgtccgcata
    15661 gccccaggta aagtaataag gtttaccctg ataaattacc gccaccgcca taccggggat
    15721 cttttgttgc tctataagcg gggtaattgt gcgatgcaca atatcgttga tttgttgagg
    15781 ggcagcaaat gtggagcaag aggcggtaat taataaggcg cagagcgtcg ttttgaacat
    15841 aaggtctggt ttccatacaa aacggcccgc cataggcggg ccggatttac attggcgatg
    15901 cgttagattg taacgacacc aatcagcgtg acaactgtca ggatagcagc cagaccgtag
    15961 aaaacccatt tgcccgcagg cacgtggatt ttcagatcgt gcatcgcgtg gtgcatacgg
    16021 tgtaaaccac accacagcgg cagaacgatc atcaggaaca ggaatacgcg accaatgaag
    16081 ctctgcgcga acgccagaac gcgctcgtag ctcagcgcat cacccggaaa cagccccagt
    16141 ggcagcagaa tacccaccag caggatcatc accggcgcaa taattgcgct ccacatacca
    16201 ccggccccga agaggcccca gaataccggt tcgtcagaac gctttggatt tggattaatc
    16261 atctcaggct ccttaccagt acagggcaac aaacaggatt acgatggtgg caaccacagt
    16321 taccgcccag agacttttga taattggctc tggtcccatt ttttcgtctt ttacaatgat
    16381 attggccgct ttcggtgcca gttcaaacca ggttttggtg tgcagcagag ctgccgtcag
    16441 agtgatcagg ttaatgatca cgataaccgg gttttgtaaa aagtcgacga atcccgccca
    16501 ggcttccggg ccatttttca gggcaaacag cccgaaaatc agttcaatgc tgaaccacac
    16561 agccggaacc gccgtgcctt cgcgcagcat gtaaaagcga taaaacggca attttttcca
    16621 ccaggtggac gtcattggcc gtacatacgg tttacgttta gtcgtcatgt tgcactcctt
    16681 agcgtggttt cagggtcgcg ataagaaagt ctttcgaact ttctactttg ccctgctgaa
    16741 tggccgcagc cggatcgacg tgtttcgggc agacttcgga gcaatagccc acgaaagtac
    16801 agctccatac accgttctgg ctgttcaact gcgccatacg ctccttctta ccgtggtcgc
    16861 ggctatcttc gttataacga tgcgccagcg taatggcagc cggaccgatg aactctgggt
    16921 tcaggccaaa ctgcgggcac gcggcgtagc acagaccaca gttgatgcaa ccggagaact
    16981 ggtgatactt tgccatctgc gccggggtct ggatgttagt accctgatcc gcggtgcggg
    17041 agttgccgat gatgtacggt ttgatcgctt ccagactttc gatgaagtgg gtcatatcga
    17101 ccaccagatc gcgttcaatc gggaagttag ctaacgcttc aaccttcata ccgtcggtgt
    17161 aatcacgcag gaaggtttta catgccagtt ttggcacgtt gttaaccatc atgccgcagg
    17221 aaccacaaat cgccatacgg caggaccagc ggtaactcag gtccggtgcc aggttgtctt
    17281 tgatgtagcc cagcgcatcc agtaatgagg tagttgcgtc ataaggcact tcatagaatg
    17341 cgctatgcgg tgcggtatcg acttccgggt tatagcgcac cacctcaatt ttcaggtttt
    17401 tcatctcagc cattcgcctt ctccttctta ttggctgctt ccgccttatc ggctgcatcc
    17461 gcttcgccac cgtaaacgcg tttagctggc ggcagcgtag taatcttcac gtcgctgtac
    17521 tccaggcgag tcgtgccatc agcatcgcgg aaggcgaggg tgtgtttgag aaagttgacg
    17581 tcgtcacgct cggtgcaacc ttcgtccaga cgctggtgtg cgccgcggga ctctttacgt
    17641 gccattgcgg agtgcgccat acattcagca acgttcagac cgtggcccag ttcaatggtg
    17701 tagagcaggt cggtgttgaa cacgctggag gtgtcggtga tgcgcacgcg cttgaagcgt
    17761 tcctgcagct ctgccagctt gtcgatggtt ttctgcatca gttccggcgt acggtagata
    17821 ccgcaacctt cttccattgc caggcccatt tcgtcgcgga tcttcgccca gttttcgccg
    17881 ccatcctggt taaccagatc tttcagacgt tgttcaacgc cagctgcctg cgcttcaatt
    17941 gccgcttcgt tgccattacc ggcagttgct gcacgctctg tcgcttgttc accggccaga
    18001 cggccgaaga ccaccagttc cgccagggag ttggagccca gacggtttgc accgtgcaga
    18061 ccaacagagg aacattcacc cacggcgaac agacctttaa tgcgggtttc acagttctga
    18121 tcggtttcga taccgcccat ggtgtagtgt gcagtcggac gtaccggaat cggttcttta
    18181 accggatcga cgccaacgta cgctttcgcc agttcgcaga tgaacggcag acgttcatgc
    18241 agttttttct cgccgaggtg acgcaggtcg agataaacca catcaccacg cggcgtggag
    18301 atggtgttgc ctttacgcca ttcgtgccag aaggcctgag aaactttgtc gcgtggaccc
    18361 agttccatat atttgttttt cggctcgccc agcggagttt ccgggcccat gccgtaatct
    18421 tgcagataac ggtagccatt tttgttgacc agaataccgc cttcaccacg gcagccttcg
    18481 gtcatcagga taccggaacc tggcagaccg gttgggtgat actgaacgaa ttccatgtca
    18541 cgcagcggaa cgccgtggct tagcgccata cccataccgt caccggtaac gatgccgccg
    18601 ttggtgttgt aacgataaac gcgacccgca ccgccggtag ccataacgac cgcgttagca
    18661 cggatctgca ccaacgtgcc ttccatcatg ttcattgcta ccaggccgcg aacatgacca
    18721 tcatcaacca gaatatccag cacgaaatgt tcgtcaaaac gctggatctg cgggaattgc
    18781 agagaggtct ggaacagcgt gtgcagcata tggaagccgg tcttatcggc ggcgaaccag
    18841 gtgcgctcga ttttcatgcc gccgaagcga cgtacgttga cgctaccatc cgggcgacgg
    18901 ctccatgggc atccccacag ttccagttgg gtcatttcgg ttgggcagtg gtggacgaaa
    18961 taatccacga catcctgctc acacaaccag tcgccacccg ctactgtatc gtgaaagtga
    19021 tattcgaagc tgtcatgatc ctgcgcgaca gcggcggagc ccccttctgc agcaacggta
    19081 tggctacgca tcgggtatac ttttgagatt agtgcgattt ttgcatttgg atttgcctgc
    19141 gcggcagcaa ttgcagcacg taatcccgcg ccaccggcgc ctacaatggc aagatcggct
    19201 tgaaaggttt gcacgacatt cctccagatt gtttttatcc cacagccccg tacttcaggg
    19261 taagtgcctg aaagttacgg tctgcgaacg ctattccact gctcctttat aggtacaaca
    19321 gtatagtctg atggataagt ctgaaatttg acgagatcga tttttttagt gcgcaagagg
    19381 gctaaattat cactgaagat gattaattta attactaaac catcagatcg tgcttttttt
    19441 agtcactgcc caccgctgtt tgattcctgc gtaaaatttg tctcgccgtc gcgttgcgag
    19501 tagacttcgt gcccttgtca aaaactggag atttaactat gagcgaaacg gcatcctggc
    19561 agccgagcgc atccattcct aacttattaa aacgcgcggc gattatggcg gagatccgtc
    19621 gtttctttgc cgatcgtgga gtgctggagg tggagacgcc ttgtatgagc caggcgacgg
    19681 taaccgatat tcatttggtc ccgtttgaga cacgtttcgt tggccccggg cattcgcagg
    19741 ggatgaatct ctggttaatg accagcccgg aataccatat gaaacgcctg ctggttgctg
    19801 gttgtgggcc ggtattccag ctgtgccgca gcttccgtaa tgaagagatg gggcgttatc
    19861 acaaccctga gttcactatg ctggagtggt atcgaccgca ctatgatatg taccggttga
    19921 tgaacgaggt ggacgatctc ttacaacagg tgctggactg cccggcagca gaaagccttt
    19981 cttatcaaca agctttcttg cgttatctgg aaattgacct gctctctgcc gacaaaacgc
    20041 aactgcggga agtcgcagcg aaactggatt tgagcaatgt tgctgatacc gaagaagacc
    20101 gcgacacgtt gctacaattg ctgtttacct ttggcgtaga gccaaatatt ggtaaagaaa
    20161 aaccgacctt tgtgtaccac tttccagcca gccaggcatc actggcgcaa atcagtaccg
    20221 aagatcatcg ggtcgctgaa cgctttgagg tttattataa aggtattgag ctggcgaatg
    20281 gtttccatga attaacggat gcccgtgagc agcaacaacg ctttgaacaa gataaccgta
    20341 agcgcgcggc gcgcggtttg ccgcagcacc ccattgacca gaatctgatt gaagccttga
    20401 aagtcggtat gcctgactgt tccggcgtgg cattaggtgt tgatcgtctg gtgatgttgg
    20461 cgctgggcgc ggagacactg gctgaagtca tcgcctttag cgttgaccgg gcataattct
    20521 gaaaaattac tgaattaaca gcgaagaatg gcgtgataag cgccattctg tagcatatgt
    20581 tttcattttt ccgttaaagg ccacaattta agactggata tttgatatca tccaggtatc
    20641 aattctgtat tggtttttac gctgcctgct caatgttgcg cagtgcagtt cagtgggcac
    20701 gtgttataca cgcgctgaaa tgaaggatgg tttcatgcct cacacgataa aaaagatgag
    20761 tctgatagga ctcatattga tgatctttac ttccgtattt ggatttgcca atagcccatc
    20821 ggcttattac ttaatgggtt atagtgcgat tcccttttat atattttctg cattgttatt
    20881 ctttattcca ttcgccttaa tgatggctga aatgggagct gcttatcgca aagaagaagg
    20941 tggtatctat tcctggatga ataatagtgt cggaccacgt tttgccttca ttggtacgtt
    21001 tatgtggttt tcctcttata tcatctggat ggtgagtacc tccgcgaaag tttgggtacc
    21061 gttctcaaca ttcctctatg gtagtgacat gacccagcac tggcgtattg ccggactgga
    21121 gcctacgcag gtggttggtc tgctggcagt agcatggatg attctggtca ccgtcgttgc
    21181 ttcaaagggg attaataaaa ttgcccgcat tactgcggtg ggcggtattg cagtaatgtg
    21241 tctgaattta gtattgctgt tagtaagcat tactattttg ttattaaatg gtgggcattt
    21301 cgcgcaggat atgaatttcc ttgcatcacc gaacccgggt tatcagtccg gtctggcaat
    21361 gctatcgttt gtggtatttg ctatttttgc ctatggcgga attgaagcgg ttggtggtct
    21421 ggtcgataaa acggaaaatc cagaaaagaa ctttgccaaa ggtattgttt ttgccgctat
    21481 tgttatttca atcggttatt cgctggcaat atttttatgg ggcgtcagca caaactggca
    21541 gcaggtatta agtaatggtt ccgttaacct tggcaatatt acctatgtgc tgatgaagag
    21601 cctcggggtg acgctgggta acgcactgca tttgtcacct gaagcgtcat tgtcgctggg
    21661 cgtatggttt gcgcgtatta ccggactttc gatgttcctc gcttataccg gtgcgttctt
    21721 tacgctttgc tattcaccgc tgaaagccat catccagggg acgccgaaag cgttgtggcc
    21781 ggaaccgatg acgcgcctga atgcgatggg gatgctgtct atcgccatgt ggatgcagtg
    21841 cgggttggtt actgtcttca ttctgctggt ttcgtttggt ggcggtaccg catcggcgtt
    21901 ctttaacaag ttgacgctga tggcgaacgt gtctatgacg cttccttacc tgttcctcgc
    21961 gctggctttc ccgtttttta aagcacgtca ggatctcgac agaccatttg tgattttcaa
    22021 aacgcgtatg tcggcaatga ttgcgacggt ggttgtcgtt ctggtggtga catttgcgaa
    22081 cgtcttcacc attattcagc ctgtggttga agccggagac tgggacagca cattgtggat
    22141 gattggcggc cctgtcttct tctcgctgtt agcgatggcg atttaccaga actattgcag
    22201 tcgcatggcg aataaacctg agttagctct cgactgatca tatgaaatcc tgcaactcat
    22261 cgtctctccg gcgatgagtt gtcaggaagt aatgcgtatg gatgatactc tccagggccg
    22321 gtgataactg aagatgatgt taagctgcta aatgagccac gttgacatca tttggcagct
    22381 tcatgcatta caaacttccc gcctgacgac ctttgcccgc agacgtcagc gttctccccg
    22441 tttgtttacc gttgaggctt tccagacgca tctggaaggg cgggaatggc atatcgatac
    22501 cgtgggcgtg gaagcctgcc agaatcagct ggtggatctc atggcgtagc ggcatacggt
    22561 gacccatctc agcggcgtaa atacgcagct cgaaaatctg aatcccctgt tgcagatcca
    22621 ccaggaagac ttccggtgcc gggttgtcga tcaccagcga gcagcgacgc gctgcggtga
    22681 gcaggatttc cgtcacttct tcactgttgg catcggcagg ggccggaatc gtcaacacta
    22741 cgcgcgtgac cgagtcagag agcgaccagt tgataaactg ctcggtaata aacgccttgt
    22801 tcggcacgat tatctcttta cggtcccagt cgctgatggt ggtggcgcgg gtgttaattt
    22861 ttgtcacgct accagtaaga tcgcggatcg ttaccgtatc gccaatgcga atcggttttt
    22921 cgaacaggat gatcaggcca gagataaagt tggcgaaaat ttcctgcaaa ccaaaaccga
    22981 gaccaacacc gagcgcggca accagccact gcaatttcga ccactcaata ccaatcattg
    23041 agaagccgac cagcccgcca atcagcatca gcagatattt tgtgatggtg gtgatggcgt
    23101 aacccgtacc cggcgttaaa tccaggtgct gcaaaatcgc cagttccagc agcgcgggca
    23161 ggttgcgcac cagctgcgtg gtgatgataa ataccagaat ggcaatcagc accgcaccga
    23221 gggtaattgg ctccagactt tctacgccct gtaccgtgga ggtgacatcc cacagcgaaa
    23281 tattttcgag gaagccgaaa gcggaatgga tttctgacca cagcacaatg accgaaagca
    23341 gggcgatcaa catcaaaatt gagcgcacca gccgcaagga ttgcgcactg atggcatcga
    23401 gatcgacttc gctttcatcg acttcaattg ctccttccgg gctactgtga tgatgcgctt
    23461 cctcttcgcc acgcgcacgt tgcgctaaca tctctgcccg gcgatgcttc gcccggtcaa
    23521 acgccagcct gcgacgctgg atcagcatcc agcggcggat aacgtgataa accaccagta
    23581 gcaggaacca gatggcaacc gaggtttcaa gcctcgctaa cagtgcctgc gccgttgcca
    23641 gataacccac cgccgacgcc agaatggcaa ccagtggtgc gccaatcatc atgttccaca
    23701 gcatatggtt ggtaatgttg tcgccgctgc cttctttgtt gagatacaac ggaataccgg
    23761 cttttttcag gcttagggtg accaccgcca gcgcacagca aatgaggata aagcaaagcc
    23821 gtcccagcga accggaaaat tcgcggtcgt cgaggttatc gaacatcatc agcgccataa
    23881 tcagcccgat gctcatcagg tagtagcgca tcccacggga aacacgttcg cgcggccagc
    23941 caaaatgagc gataaacaag ccgttcgggc gggcaaaggt ggcgcaaatc atcactaccc
    24001 acagcatcgg cacggtggcc gttacaccat cgccaatcgc gaccgccagc ggataaggcc
    24061 acgcctcgcg caagccgtaa cccagcgtca tccacagcac cggtaacggt gacgcgacga
    24121 gaatcgacca gaaaagagtg cgcaacgtca gccagaagtg atcctgagtc actttgccga
    24181 ctttcgccgc cgaacgttca agaaaacggg tgaaatagcg gcgcgagtaa atactgcaac
    24241 cgaccagaat caacgcgcca aacagcggca aaattgtctc tttgctggtc agcatcatca
    24301 cactggcttt gcccaactga ctgaaggtgt ccagcgaaat gagacgacgc agatcctggg
    24361 cgatttccag cggccaggcg atggtcatcg ggcgcacgtc agaggtccag aacagatagc
    24421 ggtgcgttgc ttcgttcacc tctttcagcg catcctccag ttgcccgttg gagactttca
    24481 gcttggtcag ttccaatagt agcgtgtcgc caccctgcaa caatgagttc agcaactcac
    24541 gctgagtgcg cagttgtgct tccagaatac ggttttgctc ggcggtcagc ggctgaccgt
    24601 cggcctgatg gatttgccgt agcagcggct gtttattaag taaatcctca taacgtaacc
    24661 gttgcacacg caactgcgac atttcggtat caagctgttg tggttttggc atttctggta
    24721 gccgtgccac ctgcgcccgc agcgcttcgc cgagcaggtt ggacgatccc agccattgcg
    24781 actgttcacg cagcgtattc aacgcctgcc ggacctgtaa cgtctggctg gcagcctgac
    24841 gctgttgcga ggcaacgaga tccatccgct gcgcctgttg attcagcgct gccgatagtt
    24901 cgcggttaat tttgaattgc gcgacgatat ctttcggcaa atcggcactg ttttctgcca
    24961 gtaattcggt actttccagc gcccgctccg cctcaagctg acgttggcta ttcaactgat
    25021 tacgtaaggc ctgcaaatac gcatccagtt gctggctctc tttttccgcc agctctgagc
    25081 gtaagcgcgc taattcctgg cggttattgg cagacagctg cgccagttcc agttcatcaa
    25141 cgagcgcctt aagacgtgca gagtcagact gcaacgcgaa attttgtgcc tgattgagcg
    25201 gagtattgcc ggtaagcgtt cccaggcggc gctcgatctc atttaactga cggcgggcgt
    25261 cggtttgctg ttgcggcagt tgattcagcg aatcggcaat ctcgcgggcg cgctcctgct
    25321 cttgctgggc ctgacggctt ttatccagca actggctgct gacctggaga atttcctgat
    25381 tcagcgcgtc ggtagacatt cccggcgaca cgctgcgcgg ctcgtcacgc atgttgttta
    25441 attgtgcgcg cagagtagcg gagagtttcg gataattatc gataacttgc tgatattgtt
    25501 tgatgcgctc aagggaacct ttgcgttcct caagcgcatt taaggcagac tggagcgcct
    25561 ctacgacttc cggctgtgcg ggtttcgccg cttttgcctg ctccagttcc tgagtgattt
    25621 gtttgctatc gggggccgtc gcggcgtacg ccccccaact gaggcaccag gccatcagaa
    25681 aagtgataat caggcgcacg tcagcgtttc ctttgatgga ttagacctag tcttttttat
    25741 cgtcaaccaa tgggctggcg tcgtgttctg cttcgatctc ttcagcagga agtggggcgg
    25801 gttcagcgtc tggcgtaaca aaggtttcgg tagatactgc cagcggctgg ccaattttcg
    25861 tgacagacag gctttccagt tgctcaacca gattcacttt acccggtgca aacaggttga
    25921 taacggtgga accgagttta aagcgaccca tttcctggcc tttcagcagt gccacagaac
    25981 cgtcgttttc cccggcaggc caggtccagc gcttggtgat accttcgcgc ggcggcgtaa
    26041 tggtgcccgc ccagaccgtc tcaatgctgc caacaatcgt cgctccgacc agaatctgcg
    26101 ccattgggcc aaattcggta tcgaaaaggc aaatcacgcg ttcgttacgg gcaaacagat
    26161 tcggcacgtt ctgagcggtg agatggttaa cggaaaagag atcgcccggc acgtagatca
    26221 tctcacgcag aataccgttg cacggcatgt gtacgcgatg gtagtcacgc ggggagaggt
    26281 aagtggtcac aaacgtaccg ttgcggaaca ggtccgccat cagatagttg catgccagca
    26341 gggcttcgag gctgtagttg tggcctttgg cttgcaggat tttatcttct tcgattttac
    26401 ccaactggct gataacgcca tcggcaggca tgaccagtac attcggatcg gtatcgattg
    26461 ggcgtacttc gtcacgcagc ggacggacaa agaattcgtt aaaggtgcgg tagctggcgg
    26521 tgtccggctt ttgcgcctct ttcatgtcga ccttgtagta tttaacgaac agatcgataa
    26581 ccagttttgt cagccatcct gcccgcttgc ttgcgcccca acccgccagg cgagtaagcc
    26641 atagtttcgg cagaatgtac tgtagcgaaa gtttaaatga atttaacaag gtagcctcca
    26701 ggccattgtt ttgtcgttcc tgatccggcc aacatgccgg atcctgaaaa aaaggggacg
    26761 attctaacga cggttagctt aattgtcagt catccgtatc agaaaagttt ttacgcgttt
    26821 ttacctgcgc catgctttcc agaatacggt gatagttttc gaaacgggtt tccgcgattt
    26881 tcccttcctc aaccgcttcc cggatagcgc agcccggatc ggtatcgtgt ttgcaatcgc
    26941 gatatttaca cagacctaaa tagtcatgga attcgacaaa gccctgagtg atttgttccg
    27001 gctccaggtg ccagaggccg aactcacgca caccagggga atcaatcaca tcaccgccgt
    27061 gcgggaagtg atacagccga gcggcggtcg tggtgtgctg accgagcccc gagttgtcag
    27121 agacatcgtt ggtcaggatc tctttttgca gccccagcaa cgcgttcagc aggctggatt
    27181 taccgacgcc agactgcccg gcaaaaatgc tgatgcgccc ggtcaacgcc tcttccagcg
    27241 gtttcagccc atcctgagta tggctggaaa ccatcaatac gcgataaccg atgttgcggt
    27301 agatatccat ctgctcgttg acgaacgcca tgccttcgtc gtccagcagg tctatcttgt
    27361 tgagcacgat aatcggctca atctgcaagg tttcgcaggc caccaggtaa cggtcgataa
    27421 tattgagcga cagctccggc aaaatggcgg agacaatgac aatctggtcg atgttggcgg
    27481 ctatgggttt cacgccgtcg tagaaatccg gacgcgttaa caccgaggtg cgctcatgca
    27541 ccgcttccac gatccctttg acatttacgc cttccgccgc cggtttaccc ggacgccaga
    27601 ctacgcggtc gccggttacc agcgaacgga tggtacggcg aatattgcag cggtgaacgt
    27661 cgccatcggc ggattccaca tcagcgtgca taccaaagcg gctgatgacg ataccttcat
    27721 caggctcgcc aaacagattg tcgtcgtagt cgggcttctc cttagacgtt ttaagacgac
    27781 gctggtgatt ggcgttcacg cggcgctgct ggcctttgga gagtttattt ttactcaatc
    27841 gtactgactc ctggtcgccc ctgatgggca aaacatctat gatacacgca attgtggatc
    27901 aatatagtca ctgtgaatgg gtggaaaata gcatgagtgc caatgaaaac aacctgattt
    27961 ggatcgatct tgagatgacc ggtctggatc ccgagcgcga tcgcattatt gagattgcca
    28021 cgctggtgac cgatgccaat ctgaatattc tggcagaagg gccgaccatt gcagtacatc
    28081 agtccgatga acagctggcg ctgatggatg actggaacgt gcgcacccat accgccagcg
    28141 ggctggtaga gcgcgtgaaa gcgagcacga tgggcgatcg ggaagctgaa ctggcaacgc
    28201 tcgaattctt aaaacagtgg gtgcctgcgg gaaaatcgcc gatttgcggt aacagcatcg
    28261 gacaggaccg tcgtttcctg tttaaataca tgccggagct ggaagtctac ttccactacc
    28321 gttatctcga tgtcagcacc ctgaaagagc tggcgcgccg ctggaagccg gaaattctgg
    28381 atggttttac caagcagggg acgcatcagg cgatggatga tatccgtgaa tcggtggcgg
    28441 agctggctta ctaccgcgag cattttatca agctgtaaaa tttttcctgg tcacgtaagc
    28501 gccggaatcc acttgcggcg gcgtgatttt gacgctaaat cgaacgtctt gctgataatt
    28561 tcagcacttg agataaaaac gcaaaaaaaa cttttttggg gggttgcaga gggaaagatt
    28621 tctcgtataa tacgcctccc gtaaagacgc agaaatgcga aaattacgaa agcaaaatta
    28681 agtagtacgc gggaatagct cagttggtag agcacgacct tgccaaggtc ggggtcgcga
    28741 gttcgagtct cgtttcccgc tccaaaattt gaaaagtgct gcaaagcaca gaccacccaa
    28801 tcgcagtatt tcgaattgcg acaaggcggc aactgaacga atccttggga acttacaaaa
    28861 gtatgtgact aaggtgagtg aaggcagcca aggcagccaa ggcagccaag gcagtagcgg
    28921 ttcgaaagac gaagat
//
LOCUS       DBKEKI010000045        28415 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.045, whole genome shotgun sequence.
ACCESSION   DBKEKI010000045 DBKEKI010000000
VERSION     DBKEKI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.045"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            390..2048
                     /locus_tag="ACUQPX_001902"
     CDS             390..2048
                     /locus_tag="ACUQPX_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HHV9056627.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2045..3001)
                     /locus_tag="ACUQPX_001903"
     CDS             complement(2045..3001)
                     /locus_tag="ACUQPX_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HHV9056628.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3152..4267
                     /gene="macA"
                     /locus_tag="ACUQPX_001904"
     CDS             3152..4267
                     /gene="macA"
                     /locus_tag="ACUQPX_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HHV9056629.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4264..6211
                     /gene="macB"
                     /locus_tag="ACUQPX_001905"
                     /pseudo
     CDS             4264..6211
                     /gene="macB"
                     /locus_tag="ACUQPX_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
     gene            complement(6284..6508)
                     /gene="cspD"
                     /locus_tag="ACUQPX_001906"
     CDS             complement(6284..6508)
                     /gene="cspD"
                     /locus_tag="ACUQPX_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HHV9056630.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6658..6732
                     /gene="yljB"
                     /locus_tag="ACUQPX_001907"
     CDS             6658..6732
                     /gene="yljB"
                     /locus_tag="ACUQPX_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HHV9056631.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6831..7151
                     /gene="clpS"
                     /locus_tag="ACUQPX_001908"
     CDS             6831..7151
                     /gene="clpS"
                     /locus_tag="ACUQPX_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HHV9056632.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7182..9458
                     /gene="clpA"
                     /locus_tag="ACUQPX_001909"
     CDS             7182..9458
                     /gene="clpA"
                     /locus_tag="ACUQPX_001909"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HHV9056633.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9861..9948)
                     /locus_tag="ACUQPX_001910"
     tRNA            complement(9861..9948)
                     /locus_tag="ACUQPX_001910"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:gga)
     gene            complement(10202..10420)
                     /gene="infA"
                     /locus_tag="ACUQPX_001911"
     CDS             complement(10202..10420)
                     /gene="infA"
                     /locus_tag="ACUQPX_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HHV9056634.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10705..11409)
                     /gene="aat"
                     /locus_tag="ACUQPX_001912"
     CDS             complement(10705..11409)
                     /gene="aat"
                     /locus_tag="ACUQPX_001912"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HHV9056635.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11451..13172)
                     /gene="cydC"
                     /locus_tag="ACUQPX_001913"
     CDS             complement(11451..13172)
                     /gene="cydC"
                     /locus_tag="ACUQPX_001913"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HHV9056636.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13173..14939)
                     /gene="cydD"
                     /locus_tag="ACUQPX_001914"
     CDS             complement(13173..14939)
                     /gene="cydD"
                     /locus_tag="ACUQPX_001914"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease/ATP-binding
                     protein CydD"
                     /protein_id="HHV9056637.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15062..16027)
                     /gene="trxB"
                     /locus_tag="ACUQPX_001915"
     CDS             complement(15062..16027)
                     /gene="trxB"
                     /locus_tag="ACUQPX_001915"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HHV9056638.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16573..17067
                     /gene="lrp"
                     /locus_tag="ACUQPX_001916"
     CDS             16573..17067
                     /gene="lrp"
                     /locus_tag="ACUQPX_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HHV9056639.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17202..21269
                     /gene="ftsK"
                     /locus_tag="ACUQPX_001917"
     CDS             17202..21269
                     /gene="ftsK"
                     /locus_tag="ACUQPX_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HHV9056640.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALLWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21424..22035
                     /gene="lolA"
                     /locus_tag="ACUQPX_001918"
     CDS             21424..22035
                     /gene="lolA"
                     /locus_tag="ACUQPX_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HHV9056641.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22046..23389
                     /gene="rarA"
                     /locus_tag="ACUQPX_001919"
                     /gene_synonym="mgsA"
     CDS             22046..23389
                     /gene="rarA"
                     /locus_tag="ACUQPX_001919"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HHV9056642.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23480..24772
                     /gene="serS"
                     /locus_tag="ACUQPX_001920"
     CDS             23480..24772
                     /gene="serS"
                     /locus_tag="ACUQPX_001920"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HHV9056643.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25012..27456
                     /gene="dmsA"
                     /locus_tag="ACUQPX_001921"
     CDS             25012..27456
                     /gene="dmsA"
                     /locus_tag="ACUQPX_001921"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HHV9056644.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNHTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINCTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27467..28084
                     /gene="dmsB"
                     /locus_tag="ACUQPX_001922"
     CDS             27467..28084
                     /gene="dmsB"
                     /locus_tag="ACUQPX_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HHV9056645.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28086..>28415
                     /gene="dmsC"
                     /locus_tag="ACUQPX_001923"
     CDS             28086..>28415
                     /gene="dmsC"
                     /locus_tag="ACUQPX_001923"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HHV9056646.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKL"
BASE COUNT         7092 a         7020 c         7663 g         6640 t
ORIGIN      
        1 atggtcgttt tatagggtcg tcgtaaaaaa tgacgacgga aataatgcgc ggctatttta
       61 aaaacgaagg cgagtcattc accagataaa taaatccagt aaatttgatt tagggcaaca
      121 gcgggttgcc ccatatagtc atttgtctga ttgacagtgt agtgcaagca aaagatttaa
      181 tcctttaggc gtaataaaaa ataatttatc atgctaatta tttgattttg ttgtttttgc
      241 gggcttatca gcaagaggga gtataacgcg attattcgct catttttcag acaattgcca
      301 tgcttaaatg tgatgtcatc acgtattagc aaggcctttc ccgttatact gccagcgtaa
      361 aggataagtc acatatttct ggaggggata tgattcttga gcgcgttgaa attgtgggtt
      421 ttcgcggtat caaccgtttg tcgttgatgc tcgaacaaaa caacgtcctg attggggaga
      481 acgcgtgggg taaatccagc ttgctggacg ccttaaccct gctgctatcg ccagaatcag
      541 atctctacca ttttgagcgc gacgatttct ggttcccgcc gggagatatc aacgggcgag
      601 aacatcatct gcatattatt ttgaccttcc gcgaatcgct gccaggccga catcgggttc
      661 gccgttatcg gccgctggaa gcgtgctgga cgccatgcac cgatggctat caccgtattt
      721 tttatcgtct ggaaggggag agtgcggaag acggcagcgt gatgacactg cgcagttttc
      781 tcgataaaga cggacatccg attgatgttg aggatattaa cgatcaggca cgccatctgg
      841 tgcgtttaat gccggtgctg cgcttgcgtg atgcccgttt tatgcgccgt attcgtaacg
      901 gcacggtgcc aaatgtcccc aatgtggaag tcaccgcgcg ccagctcgat ttcctcgccc
      961 gtgagttgtc ctcacatccg caaaatctct ctgatggaca gattcgtcag ggactttccg
     1021 caatggtgca gttgcttgag cattatttct ctgagcaggg ggctggacag gcgcgatatc
     1081 gtttaatgcg gcggcgggcc agcaatgagc aacgaagctg gcgctatctg gacatcatca
     1141 accggatgat tgaccgacct ggagggcgct cgtatcgggt tattttgctc ggcctgtttg
     1201 ctactttatt gcaggcaaaa ggcacattgc gactggataa agacgcgcgt ccgttattgc
     1261 taattgaaga tccagaaacc cgcctgcatc cgattatgct ttccgttgcc tggcatctgt
     1321 tgaatctttt gcctttacag cgtattgcta ccaccaactc tggcgagctg ctttcgttaa
     1381 ctccagtgga acatgtttgc cgcctggtgc gtgaatcttc tcgtgttgcc gcctggcgtc
     1441 tggggccaag tggcttgagc accgaagaca gccgccgcat ctcttttcac attcgtttta
     1501 atcgtccgtc atcgctgttt gctcgctgct ggttgctggt ggaaggggaa acggaaacct
     1561 gggttatcaa tgaactggcg cgtcagtgcg gacatcattt tgatgccgaa gggatcaagg
     1621 tcattgagtt tgcccagtcc gggctaaagc cactggttaa atttgcccgc cgaatgggga
     1681 ttgaatggca tgtactggtc gatggcgatg aagcagggaa gaaatatgcc gctacggtac
     1741 gtagcctgtt gaataacgat cgggaagccg aacgagaaca tttaacggcg ttaccggcgc
     1801 tggatatgga acattttatg tatcgccagg gattttccga tgtgttccac cgcgtggcgc
     1861 aaatcccgga aaatgtaccg atgaatctac gcaaaattat ctcgaaagcg atccatcgct
     1921 cttccaaacc cgatcttgcc attgaagtgg caatggaggc aggacgtcgt ggtgtggact
     1981 ccgtaccaac gctgctgaaa aaaatgttct cacgcgtgct gtggctggcg cgcggtcgcg
     2041 cggattaacc gcgaaacatc gtggccattt gtggctgaat agcgtcgagc atctcatagc
     2101 gccgacggta ttcagcccgt tttttactgg cgatttcggc aatctctttt cgtgctatct
     2161 gtgctggaag gcggtaatgg cgttcagcat cacatacgcc gccaaccgac tcccagaacg
     2221 cgttgtaatc agcatggatc ttgccttctt tatcgcgata acgcaggctg cggtaaatat
     2281 gcgtttcatt gctgacggca ataatctgct ctacctgcaa acgttgggca aacagacagg
     2341 ccgcttccat cacgaggcgt ttgggaaata gcccgtggca ggctttcgtc gcattctgga
     2401 tttcctgatg tggaatttcc cattttgcgc cttgcagtcc gccaataaac atcgttcttt
     2461 tcccctgata ttcacacagg gtaaacgtga tctctgccag aggaatacct tcgctgttgc
     2521 ggaacaggat tgtgctgtca ccttctttat ccattgagat catcatggtc agctcaagcg
     2581 tgaactgctc accgtttttg ccttccagtt tagccagttg cagcccgggg gtattcaaat
     2641 ataagctgaa ttcttccgcc gacatacacc cgcggagtaa cgcataatgg taacgtaacg
     2701 cctccagcaa ttgcttacgg ctaagattcg ccgcaaggta agggcgatgc agacgcacag
     2761 gcagtcgcgg ctggcgcgtt aacaacacat tgagattagg ccagtgggaa agttcgttca
     2821 tccactcaac gcttaaacgc ggcataatca acgagcgcag caaaaatttc tggcgaaaac
     2881 tacagcgatg ccagaattta cccggccgac actgtccacg tgccagacta agaaaaagtg
     2941 acaggctgct gagagattca gatggcgtaa aggtccgttc agttagctgc gacatattca
     3001 tgaaatcaat ggttatacat gacgtcgatt tcaccattgc gtatcttaac caaacatcaa
     3061 tagtgtgatt actaacgtaa attttagggt tttgttgata tttcgttgaa gttaatgacc
     3121 cggattggca tatggagtat tcagaatatt tatgaaaaag cggaaaaccg tgaagaagcg
     3181 ttacgttatt gcgctggtga tagtcatcgc cggactgatt acgttatgga gaattcttaa
     3241 cgcacccgtg ccgacttatc agacactgat tgtgcgcccc ggtgatttac agcaaagcgt
     3301 gctggcgacc ggaaagctgg acgcgctgcg taaggttgac gtgggcgcgc aggtcagcgg
     3361 tcagttgaaa acgctgtcgg tggcgattgg cgataaagta aaaaaagacc agcttttagg
     3421 ggtcattgat cctgaacagg ctgaaaacca gatcaaggag gtcgaagcaa cgctgatgga
     3481 gctacgcgcg cagcggcagc aggcggaagc ggagctgaaa ctggcgcggg tgacgtattc
     3541 ccgtcagcaa cgtctggcac aaacgcaagc ggtttcactg caggatctcg ataccgccgc
     3601 gacggagatg gctgtgaaac aggcgcaaat tggcaccatt gacgcgcaaa tcaagcgcaa
     3661 tcaggcctct ctcgatacgg ctaaaaccaa tctcgattac acccgtatcg ttgccccgat
     3721 ggccggggaa gtgacgcaaa tcaccactct gcaaggccag acggtgattg ccgcacagca
     3781 agcaccgaac attctgacgc tggcagatat gagcactatg ctggtaaaag cgcaggtttc
     3841 tgaagcggat gtaatccacc tgaagccggg gcaaaaagcc tggtttacgg tgcttggcga
     3901 tccactgacg cgctacgagg ggcaaatcaa ggatgtgcta ccgacgccgg aaaaggtaaa
     3961 cgacgctatt ttctattacg cccgttttga agtacccaac cccaatggtt tgctgcggct
     4021 ggatatgacc gcgcaagtgc atattcagct caccgatgtg aaaaatgtgt tgacgatccc
     4081 tctgtcggcg ttaggcgatc cggttggcga taatcgttat aaagtcaaat tgttgcgtaa
     4141 tggtgaaaca cgcgagcgtg aagtgacgat tggcgcacgt aacgataccg atgttgagat
     4201 tgtcaaaggg ctggaagcgg gcgatgaagt ggtgattggt gaggccaaac caggagctgc
     4261 acaatgacgc ctttgctcga attaaaggat attcgtcgca gctatcctgc cggtgatgag
     4321 caggttgagg tgctgaaggg catcagcctc aatatttatg cgggtgagat ggtcgcgatt
     4381 gttggcgctt cgggttccgg taaatcgacc ctgatgaata ttctcggctg cctggataag
     4441 gccaccagcg gcacctatcg cgtagccggt caggatattg ccacgctgga cgccgatgcg
     4501 ctggcgcaac tgcgccgcga gcatttcggc tttattttcc agcgttacca tttgctttcg
     4561 catttaaccg ccgagcagaa cgttgaagta cccgccgtct atgccggtct tgagcggaaa
     4621 cagcgactgc ttcgtgccca ggagttgctg caacggctgg ggctggaaga ccgtacagag
     4681 tattatccgg cacagctttc gggtggtcgg caacagcgcg tcagcatcgc gcgggcattg
     4741 atgaacggcg gtcaggtaat tcttgccgat gaaccgaccg gcgcactgga cagccattct
     4801 ggcgaagagg tgatggcgat cctgcatcag ctgcgcgatc gcgggcatac ggtgattatc
     4861 gtcacccacg atccgcaggt cgctgctcag gccgagcggg tgatcgaaat tcgcgacggc
     4921 gaaattgtgc gcaatcctcc cgccattgaa aaagtgaatg ttgctggcgg gacggagcct
     4981 gttgtcaata cagtgtctgg ctggcggcag tttgtcagcg gttttaacga ggcgctgacg
     5041 atggcatggc ggacgctggc agcgaataaa atgcgtactt tactgaccat gctggggatt
     5101 attatcggta ttgcgtcggt ggtttccatt gtcgtggtgg gtgacgccgc caaacaaatg
     5161 gtgctggcgg atattcgttc tattggtacg aatactattg atgtctatcc cgggaaagat
     5221 tttggcgatg acgatccgca atatcagcag gcgctgaagt acgacgactt aatcgccatc
     5281 caaaaacaac cgtgggtcgc ctcagccaca cctgccgtct cgcaaaacct gcgcctgcgt
     5341 tataacaatg ttgatgttgc tgccagtgcc aatggcgtga gcggcgatta ttttaatgtc
     5401 tatggcatga cacttcagtg aaggaaacac ctttaatcag gagcagctga acggtcgtgc
     5461 gcaggtcgtg gttctcgaca gaaatactcg ccgccagctt ttcccccata aagcagatgt
     5521 ggttggcgag gtgattctgg tcggcaatat gcccgccaga gtcattggtg tggcggaaga
     5581 aaaacagtcg atgtttggta gcagtaaagt gctgcgtgtc tggctacctt acagcacgat
     5641 gtccgggcga gttatgggcc agtcgtggct taactccatt actgtcaggg tgaaagaagg
     5701 atttgacagc gccgaggcgg aacagcaact cacgcgttta ctttcactgc gccacggtaa
     5761 gaaggatttc tttacctaga acatggacgg cgtcttgaaa actgttgaaa agaccacacg
     5821 tactttacaa ctgtttctga cgctggtggc ggtgatttcg ctggtggtgg gcggtattgg
     5881 tgtaatgaat attatgctgg tgtcagtgac cgagcggacg cgggaaattg gcattcgcat
     5941 ggctgtaggt gcgcgagcaa gcgatgtttt gcaacagttc ctgatcgaag ccgtactggt
     6001 ttgcctggtc ggtggcgcgt tgggaataac actgtcactg ttaattgctt tcaccttgca
     6061 gcttttctta cccggctggg agattggttt ttcaccgttg gcgctgctgc tggcgtttct
     6121 ctgctcgacg gccaccggga ttttatttgg ctggttaccc gcacgaaatg cggcacgact
     6181 ggatccagta gatgctctgg cacgagagta atttttgaga taaaaatgcc agccgatcgg
     6241 gctggcattt tgcctttagg atgtacacaa tgagacagaa gagctatgcg actgccgctt
     6301 ctacttcgac gggcacaata acactggcgt gattgccttt tggcccctgg tggacatcaa
     6361 actgaacgga ttgtccagct tttagcgttc tgtaaccatc catctgaatg gtggaataat
     6421 gagcgaaaat atcttcgccg ccgccttcag ggcagatgaa accaaaccct ttggcattgt
     6481 tgaaccactt aacagtaccc ttttccatgc ttcgacatcc ttcgcaaatc ttatacaagt
     6541 aagatggaat aaaccggggt cagagagggg gctgttcaaa acctcgccaa ctctagaaat
     6601 acaatttaga gaattagggc gaaccgtcaa gcatttgaca ggggccaggg ggcaggtatg
     6661 aatcaaaaat ttgaagcagt taacgctatt gacaggaatg tgacagatgt cgctgatgcc
     6721 aacgatagat gatagttatc tatcatgtgg agtagattgg tcaggcaaat aagctcttgt
     6781 cagcggcagg gcgttctgcc gataaccgta accgaagatg ataactgaca atgggtaaaa
     6841 cgaacgactg gctggacttt gatcaactgg cggaagaaaa agttcgcgac gcgctaaaac
     6901 cgccatctat gtataaagtg atattagtca atgatgatta cactccgatg gagtttgtta
     6961 ttgacgtgtt acaaaaattc ttttcttatg atgtagaacg tgcaacgcaa ttgatgctcg
     7021 ctgttcacta ccaggggaag gccatttgcg gagtctttac cgccgaggtt gcagaaacca
     7081 aagtggcgat ggtgaacaag tacgcgaggg agaatgagca tccattgctg tgtacgctag
     7141 aaaaagcctg aatgcaggca taaaaattgg gggaggtgcc tatgctcaat caagaactgg
     7201 aactcagttt aaatatggct ttcgccagag cgcgcgagca ccgtcatgag tttatgaccg
     7261 tcgagcactt gttactggcg ctgctcagta acccatctgc ccgggaggcg ctggaagcgt
     7321 gttctgtgga tttggttgcg ctccgtcagg aactggaagc ctttattgaa caaaccacac
     7381 ccgttctgcc tgccagtgaa gaggagcgcg acacacagcc gacgctgagt tttcagcgtg
     7441 tactgcaacg tgcggtcttc catgtccagt cctccggtcg caatgaggtt accggtgcaa
     7501 acgttctggt cgctatcttc agcgaacagg agtcgcaggc ggcatatctg ttgcgtaaac
     7561 atgaagtcag ccgtctcgat gtggtgaact ttatctctca tggcacgcgt aaagacgagc
     7621 cgacacagtc ttctgatcct ggcagccagc caaacagcga agaacaagct ggtggggagg
     7681 aacgtatgga gaatttcacg acgaacctga accagcttgc gcgtgtgggc ggaatcgacc
     7741 cactaattgg tcgtgagaaa gagctggagc gtgctattca ggttctctgc cgtcgccgta
     7801 aaaacaaccc gctgctggtg ggggaatctg gtgtcggtaa aaccgcgatt gcagaaggtc
     7861 ttgcctggcg aattgttcag ggtgatgtgc cggaagtgat ggctgactgt acgatttact
     7921 ctctcgatat cggttctctg ttagcgggca caaaatatcg cggcgacttt gaaaaacgtt
     7981 ttaaagcgtt gctcaagcag ctggagcagg acactaacag catcctgttt attgatgaga
     8041 tccacaccat tatcggtgcg ggtgcagcgt ctggtggtca ggtcgatgcg gctaacctaa
     8101 tcaaaccgtt gctctccagc ggtaaaattc gcgtaattgg ttcgacaacc tatcaggagt
     8161 tcagcaacat tttcgagaaa gaccgtgctc tggcgcgtcg cttccagaaa attgatatta
     8221 ctgaaccgtc gatcgaagaa actgttcaaa tcatcaatgg cctgaaaccg aagtatgaag
     8281 cgcaccacga cgtgcgttat accgcaaaag cggtgcgtgc ggcggtagag ctggcggtga
     8341 aatacattaa cgatcgtcat ctgccggata aagccattga tgttatcgac gaagcgggcg
     8401 ctcgcgcacg tctgatgccg gtaagcaaac gcaagaaaac cgttaatgtg gcggatattg
     8461 agtccgtggt ggcccgtatt gcacgcattc cagagaagag tgtttctcag agtgaccgcg
     8521 ataccctgaa aaacctcggc gatcgcctga aaatgctggt cttcggccag gataaagcca
     8581 ttgaggcgct gactgaagcc attaagatgg cgcgtgcagg tttaggtcac gaacataaac
     8641 cggtcggttc gttcctgttt gccggtccta ccggggtcgg gaaaacagag gtgacggtac
     8701 agctttcgaa agcgttgggc attgagcttc tgcgctttga tatgtccgag tatatggaac
     8761 gccataccgt cagccgtctg attggtgcgc ctccgggata cgttggtttt gatcagggcg
     8821 gtttgctgac cgatgcggta atcaagcatc cacatgcggt gctgctgctg gacgaaatcg
     8881 agaaagcgca tccggacgtg ttcaatattc tgttgcaggt gatggacaac ggtacgctga
     8941 ccgataacaa cggacgcaaa gcggacttcc gtaacgtggt gctggtgatg accactaacg
     9001 ccggggtacg ggaaactgag cgcaaatcca ttggtcttat ccaccaggat aacagcaccg
     9061 atgcgatgga agagatcaag aagatcttta caccggaatt ccgtaaccgt ctcgacaaca
     9121 ttatctggtt cgatcatctg tcaactgacg tgatccatca ggtggtggat aaattcatcg
     9181 tcgagttgca ggttcagctg gatcagaaag gtgtttctct ggaagtgagc caggaagcgc
     9241 gtaactggct ggccgagaaa ggttacgacc gggcaatggg cgcacgtccg atggcgcgtg
     9301 tcatccagga caacctgaaa aaaccgctcg ccaacgaact gctgtttggt tcgctggtgg
     9361 acggcggtca ggtgacggtt gcgctggata aagagaaaaa tgagctgact tacggattcc
     9421 agagtgcaca aaagcacaag gcggaagcag cgcattaatc tgattgtcag gtaggttggt
     9481 gaagtccgta atctcgtcag gggttacgga ctttttattt atggggtgga acggtgaaaa
     9541 ccctattttt ggaggtgaag gtaagttgtt gataattagt gctgctggaa ggtaaggata
     9601 aaaaagggtg ctgcaggaga atgggatggt tttgctttat taacaacggg ctaaacgtgt
     9661 agtatttgag ttcactgccg tacaggcagc ttagaaatcg acggggtgcg gtaaaacctt
     9721 tgcgaacgcg ttcactgccg tacaggcagc ttagaaattc acaggtaaca tactccaccc
     9781 gcccaccatg ttcactgccg tacagacaga taaaatgcga aaaaaaagct cgcactttcg
     9841 tacgagctct tctttaaata tggcggtgag ggggggattc gaacccccga tacgttgccg
     9901 tatacacact ttccaggcgt gctccttcag ccactcggac acctcaccaa attgttttgc
     9961 taccaaacct catgggtggc aacggggcgt tactataggg agttggagta aaacggtcaa
    10021 gaagaatttt aatgataatt attgtttgct catactgtaa acaacttgtg cagtatatct
    10081 acatcgagac aagttatgga cttatacttc caaagtactt catacatatc acaaaataaa
    10141 aaggccggtt aaaccgacct tttactcgtt ctttctcttc gcccatcagg cggtaaaaca
    10201 atcagcgact acggaagaca atgcggcctt tgctcaggtc gtacggggtc agttcaacag
    10261 tcactttgtc gcccgtcagg atgcggatgt agtttttgcg cattttaccg gagatgtgtg
    10321 cagtaaccac gtgaccgttt tctaactcta cgcggaacat ggtattaggc aacgtttcaa
    10381 gaacggtacc ttgcatttca atattgtctt ctttggccat ctaatcctct ggggtatcac
    10441 taccgtaatt tgaaccggca agataatgcc gaagttctgt aaataagtaa agatttgcgc
    10501 gctaaatcgc aacaaacagg ttcggcacat tactccgaaa acacacggct aagccgcacc
    10561 aaaagcgcaa cgtataaggg agcggtgaga taaacgatgg gcgttacctg acgcgaaaaa
    10621 ttccttatcg gcagcggggt aatgagcgta accaactctg cgaccgcaat tataacactc
    10681 tggggagaaa tgtgccgaaa acattcattc ttgtggtgaa aacaagcacc gtggtaccca
    10741 gaaattattc ggcaatcgtc cgaggcgcat ttgattgaga taattaaggt aatcccggcg
    10801 gggaatttcg caggcaccaa gcgatgctgt gtgatcgtta aggacctggc agtcgataag
    10861 cttaccgcca tgaccgataa attcctcaca gaataccaga agcgccgttt tagacgcatt
    10921 ttccatccgg ctgaacatgg actcgccaca aaatagcgtt ccctgggcca cgccgtacat
    10981 accgccgaca agctcatctt cacgccagac ttcaatggag tgggcatgac cgagttcgtg
    11041 aaggcgatgg taggcttcga ccacgccacg cgtgatccag gttccttctt cgcgatcgct
    11101 ggcacagcct tcaatgacct gaccaaaagc gtaattcatc gtgacacgat agggcgagcg
    11161 tttatgaaat cgcttcatac tacggctgat atgcagtgat tctggccata gcaccgcgcg
    11221 gggatcgggc gaccaccaga ggatggggtc gcctggagaa aaccacggaa aaataccacg
    11281 ctggtaagcc attaacaggc gcgcagggct aagatcgccc ccaagtgcca gcaggccgtt
    11341 aggctcacgt aatgcgcctt ccggggaagg gaaggctatt gaatggcgag aaagctgaac
    11401 caggcgcatg accgcaaaac tccacgcaag tcggatcgtt caataatagc ttacaaaccc
    11461 tgcttgaact ggtaataacg cccctgtctg gcaagcagtt ctgcgtgagt accttgctca
    11521 ataatttgcc cgttgtccat cactattatt tgttggaaac gagagagtcc gcgaagtcga
    11581 tgggtgacca ttaacaccgt tttctcacgc atcatttctg caagcaattc aaggatctgg
    11641 ctttcggttg tggcatctaa gccttcggta ggttcatcca gcaacaccag tggcgcatca
    11701 tgtaacagcg cacgggcgat agccagacgg cgcagttcac caccggagag ctggcgtccg
    11761 ccttcaccta accaactgtt gagacctgca tcctcgagca gcttctccag accaacgcga
    11821 cgcaagatct ccgacagagc ctcatcacta ctgccaggcg aggcgagtaa cagattatca
    11881 cgcagcgtgg cgctaaacag atgcactcgc tgaggaacaa cgctgatggt ctgtcgtaga
    11941 gccgcttcat tcaggctggc tatggggcta tcgttaagca aaatctcgcc ctgttgcggg
    12001 tcccatgcgc gggtcagcag ttgtaacagt gttgatttgc cgcatccggt tcgcccgaga
    12061 atcgctatat gttccccggc gtttacctga agagaaatcc ctttaagtgc ctgttgggat
    12121 tgctccggat aagtgaactg aacatcccgt aatgtcagcg aaacgcgatc ggcaacacga
    12181 gtttgggtat cagggaaggt gacctccggt ttttgatccg ttaagtcaga gatacgtacg
    12241 gcagaggcaa tgacttgccc cagatgctga aatgcacccg ttactggtgc cagtgcttca
    12301 aacgcggcta acgcacaaaa gacaaacagg gcaattaatg cgccgggttg agcattgccg
    12361 ccaacgccgc cagacgccat ccacagcatc aggatcaccg ctaacgcgcc aatgagcagc
    12421 attatcgctt gcgacaatgc ggtcagttca gattgacggc gttgcgcttc cagccattga
    12481 atttctgtat tctctagttg cgtgcgataa cgatcgctgg caccaaaaat ggtcagctca
    12541 gcttgccctt gcagccaggc agtcagttgt tggcgatact gtccgcgaag atgagtcaga
    12601 ttttgcccgg tgctttttcc cgcacgataa aacagcggtg gcatcaggaa aagcgtcagt
    12661 aacataacgc tgcccagcgt aaaggcgagg gtgaaatcaa ggaaacttaa cccgattgtc
    12721 accaccataa tcaccacaaa agcgcccacc agcggcgaga taacgcgcag gtaaagatga
    12781 tcgagcgtat caacatccgc caccacgcgg ttgagcaatt cgccctgacg atagcgcgcc
    12841 agtccggcag gggagagggg caacaatttg ctgaaggtgt aaatgcgcag atgctgcaac
    12901 acgcggaaag tcgcgtcgtg acttaccaga cgttcaaaat aacgcccggc agtacgggtg
    12961 attgcggcac cacgcacgcc cgcagcgggt agcatatagt tgaagctgta cagtccggca
    13021 acccccgcaa ccgctgaggc cgagaggaac cagccggaaa gtgtcaacag accgatactg
    13081 gcgagcagcg tcacaattgc cagcacaata ccaagactta acatccattt atgacgttta
    13141 tacagtgcca gatagggtag caaagcgcgc atttaaatct cctcctgacg atgggccagt
    13201 aatgtggcga atgggccacc agccacactt aattccgcgt aacgtccttg ctcaataatc
    13261 cggccatcct gcataaccca aatgacatcc cagtcagcaa gatcttctaa ctggtgggtg
    13321 accattaacg ttgtctggcg cagagaggcg gcattcagcg cctccattac gcgctgttca
    13381 ctgtgagcat caaggctggc agcgggttca tccaacagta atagcgaaca gggatttagt
    13441 aacgcacggg ccaccgccac gcgctgcgcc tgccccacgg aaaggcgggc agcctggtcg
    13501 ccaacaggtg tatcaacgcc ttgtggcagg agcggtagaa actcgctgac ccaggcgtta
    13561 tccagcgctg cttgtaattc ttgttcgctg gcatcaggtc gcgccagtag tacgttatcc
    13621 cgcaattttg ctgccggtaa ttgtgggttt tgcccaaccc aggagagatg tttacgccat
    13681 gattctggtg ataaatcacg taattctatc ccgttgattc gtaatgatcc ctgatatgag
    13741 agaaaaccag aaagcgcgtt cagcagtgag cttttacctg aaccgctgcg gccaaccaac
    13801 actgcacgtt ggcctgctgg caaagtaaag ttcagcggtc cggccagcgt tttaccttcc
    13861 ggcgacgtga taaacagatc ctcggcctca atggtcagcg gatcggtcaa tgctaattcc
    13921 gcctcaccgc gctgcggatg ggcgagcggg gtttccataa acgttttcag actgtcagct
    13981 gcaccaacag cctgggcttt agcatgataa aacgtaccga gatcgcgtaa tggctggaaa
    14041 aactctggcg caaggatcag ggccagaaaa cctgcagcca gcgtcacccc agtatcgtag
    14101 tgaccaaaat ccagctcgcc gagataggaa aaaccaaagt agaccgccac cagagcaatt
    14161 gacagcgagg taaaaaattc gagaatgccg gaggataaaa acgccagtcg tagcacttcc
    14221 attgtccgtt ggcggaaatc ttccgaagca gaacgaatac tttcaatttc agcttcacca
    14281 cgaccaaaaa tacgcaatgt ttccatgccg cgcaggcgat cgaggaaatg cccacttaag
    14341 cgagcaagag cgagaaagtt acgccggtta gcatcggcag cccccattcc aaccagcgcc
    14401 ataaacaacg gaattagcgg tgctgtgccc agcagaatga gcgccgcagc ccagttagag
    14461 gggaagatcg ccaccacaat cagcaacggc accgacactg ccagcgccat ttgcggcaga
    14521 tagcgtgcat agtaatcatg catatcgtca atttgctcga gtaccagcgt cgcccagctc
    14581 cccgcaggtt taccctgaat ccacgctggc cctgcttgtt gcagacggtc gagaacctga
    14641 cggcggatgg caaagcggat atgctgcccg gcgtgataac ccacccgttc gcgtaaccag
    14701 accacccatg cgcgcaatac aaaggtcaga accagtaacg taaagggaag cagcagggct
    14761 tcacggggaa tattctccat aatcatatgt tgcagaattc gcgccatgaa ccaggcctgg
    14821 gcaatgatca atatgccgct tacaaagccc agcagacgag aaatattcag ccaacgttgg
    14881 gagatgacgc tttgctgttt taaccagcgg gttaactctt tttgacgaga tttattcatt
    14941 gcacgcttag caggtgagtt atcagaatta tttgcagagc aatgttacaa cggggaaaaa
    15001 ataaaggcga cccatagtcg caaggtgtcg ccttctttac ttttgttact gatttgtaaa
    15061 attattttgc gtcagctaaa ccatcgaggt agcgttccgc atcaagtgct gccatgcagc
    15121 cagtaccggc cgaagtaatg gcctggcgat aaatgtgatc catcacgtcg cctgcggcaa
    15181 agacgccagg aatgctggtc tgggtggcat taccatgaat acccgactgt actttgatgt
    15241 agccgttttc cagttccagc tgcccttcga aaatcgcagt attcgggctg tgaccgatag
    15301 caacaaacag accggcaacg tcgagtgact cgatgttatc gctgttttgc gtatcgcgca
    15361 gacgaacacc agtgacaccc atttgatcgc cggtcacttc ttccagcgta cggttggtgt
    15421 gcagaatgat gttgccgttc tccactttat ccatcagacg cttaataagg attttttccg
    15481 cgcggaaacc gtcacggcgg tgaatcagat gcacttccga agcgatgtta gacagataca
    15541 gcgcctcttc aaccgcggta ttgccgccgc cgatgaccgc aactttctgg ttgcgataga
    15601 agaaaccgtc gcaggttgca caagcagaaa ccccacggcc tttaaaggct tcttcagagg
    15661 gcaggccgag atagcgtgca gaagctccgg tggcaataat cagcgcgtcg caagtgtatt
    15721 cgccgttatc gccattcaga cggaacggac ggttttgcag atccaccttg ttgatatgat
    15781 caaaaatgat ctcagtttca aacttggtgg catgttcgtg catgcgctcc attaataacg
    15841 gaccggtcag atcgtttgga tcaccaggcc agttttccac ttccgtggtg gtggtcagtt
    15901 ggccgccttt ttccatgccg gtaatcagca caggttgcag gttggcgcgc gccgcgtaga
    15961 cagcagcggt gtatcccgcc gggcctgaac ccaggataag cagtttactg tgtttggtcg
    16021 tgcccatgag atccccatag ttgttggcag acaatgggca ggattgtagg gaatttacag
    16081 acgtaaaaaa agagtatgac gattttgtta acaatttgtg caatcggcag catcgataag
    16141 caggtcaaat tctcccgtca ttatcacctc tgctacttaa atttcccgct ttataagccg
    16201 attaaatgat gaataaacgc ccctgttaat gaatatctgg catgttgtac taaaaatcga
    16261 tgttttgctt tgacaatccc ctggtgtttt gcgaaaacat tcgaggaaga aaaaaaaaca
    16321 gtattcttat atgcgcataa ccatgtatgt aaatatcatg tttaccgtgc tagtgaaatc
    16381 tacgtatggc gtggacagac gccatacgtg atgtcgatag ctgccacaag gcaacggtct
    16441 tctcaccgta gacccaggca ttgcgcgccg tgaatcttca tgatttcggt ctatcgtgac
    16501 gggtagcgac tctgaacagt gatgtttcag ggtcagacag gagtagggaa ggaatacaga
    16561 gagacaataa taatggtaga tagcaagaag cgccctggca aagatctcga ccgtatcgat
    16621 cgtaacattc ttaatgagtt gcaaaaggat gggcgtattt ctaacgtcga gctttctaaa
    16681 cgtgtgggac tttccccaac gccgtgcctt gagcgtgtgc gtcggctgga aagacaaggg
    16741 tttattcagg gctatacggc gttgcttaac ccccattatc tggatgcatc acttctggta
    16801 ttcgttgaga ttactctgaa tcgtggcgca ccggatgtgt ttgaacaatt caataccgct
    16861 gtacaaaaac ttgaagaaat tcaggagtgt catttagtat ctggtgattt cgactacctg
    16921 ttgaaaacac gcgtgccgga tatgtcagct taccgtaagt tgctggggga aaccctgctg
    16981 cgtctgcctg gcgtcaatga cacacggaca tacgttgtta tggaagaagt caagcagagt
    17041 aatcgtctgg ttattaagac gcgctaacac ggaacaggtg caaaatcggc gtattttgat
    17101 tacactcctg ttaatccata cagcaacagt actggggtaa cctggtactg ttgtccgttt
    17161 tagcatcggg caggaaaagc ctgtaacctg gagagccttt cttgagccag gaatacactg
    17221 aagacaaaga agtcacattg acaaagttaa gtagcggccg ccgccttctg gaggcgttgc
    17281 tgatccttat tgtcctgttt gccgtctggt tgatggctgc cttactaagc tttaaccctt
    17341 cggaccccag ctggtcgcaa acggcctggc atgaacctat ccataattta ggtgggatgc
    17401 ccggtgcgtg gttggcagat acgctgttct ttatttttgg cgtgatggct tacaccattc
    17461 ccgtcattat tgtcggcggt tgttggtttg cctggcgtca tcagtccagc gacgaataca
    17521 ttgattattt tgccgtttcg ctacgcatca ttggcgtttt ggcgctcatc cttacctcct
    17581 gtggtctggc ggcaatcaac gctgacgata tctggtattt tgcctccggt ggcgtcattg
    17641 gcagcttact aagcactacg ctacaaccac tgctacacag tagcggggga actattgcgc
    17701 tgctctgcgt ttgggcagcg ggcctgacgt tgttcaccgg ttggtcatgg gtgaccattg
    17761 ctgaaaaact cggcggctgg attttaaaca ttctcacctt cgccagtaat cgtacccgtc
    17821 gcgatgatac ctgggtcgat gaagatgagt atgaagacga cgaagagtat gaagatgaaa
    17881 atcacggcaa acagcatgaa tcacgccgtg cccgtattct tcgcggcgcg ctagcgcgtc
    17941 gtaaacggtt ggcggaaaaa ttcattaatc cgatggggcg gcaaacagac gctgcgttgt
    18001 tctccggtaa gcggatggat gatgacgaag agattaccta cactgcacgc ggtgtggctg
    18061 ccgacccgga cgacgtccta ttttcgggca atcgtgcaac gcagccagaa tatgacgaat
    18121 acgatccatt attaaacggt gcgccaatta ccgaacctgt cgctgtagca gctgctgcta
    18181 ccacggcgac acaaagctgg gctgcgccgg ttgaacctgt gactcagacg ccgcctgttg
    18241 cctctgttga tgttccacct tcgcaaccta cagtagcctg gcagcctgta ccgggtccac
    18301 aaacgggaga gccggttatt gctcctgcac cggaaggtta cccacagcag tcacaatatg
    18361 cgcagcctgc agtgcaatat aatgagccgc tgcaacaacc agtacagccg cagcagccgt
    18421 attatgcacc tgcagctgaa cagcctgcgc aacagccgta ttatgcccct gcgccagaac
    18481 aaccggtggc aggtaacgcc tggcaagccg aagagcagca atccactttt gctccacagt
    18541 ctacatacca gactgagcaa acttatcagc agccagccgc tcaggagccg ttgtaccaac
    18601 agccgcaacc cgttgaacag cagcctgttg tggagcctga acccgttgta gaagagacaa
    18661 aacccgcgcg tccgccgctt tactattttg aagaagtgga agagaagcga gcccgtgaac
    18721 gtgaacaact tgcggcctgg tatcaaccga ttccagaacc ggttaaagaa ccagaaccga
    18781 tcaaatcttc gctgaaagca ccttctgttg cagcagtacc tccagtagaa gccgctgccg
    18841 ctgtttctcc gctggcatct ggcgtgaaaa aagcgacact ggcgacgggg gctgccgcaa
    18901 ccgttgccgc gccagtcttc agtctggcaa atagcggtgg accgcgtccg caggtcaaag
    18961 aggggattgg cccgcagttg ccacgaccga aacgtatccg cgtgccaact cgtcgtgaat
    19021 tggcgtctta cggtattaag ctgccctcac agcgtgcggc ggaagaaaaa gcccgtgaag
    19081 cccagcgcaa tcagtatgat tctggcgatc agtataacga tgatgaaatc gatgcgatgc
    19141 agcaggatga actggcacgt cagttcgccc agacacagca gcaacgctat ggcgaacagt
    19201 atcaacatga tgtgcccgta aacgcagaag atgcagatgc tgcggcagag gctgaactgg
    19261 ctcgtcagtt tgcgcaaact caacaacaac gttattccgg cgaacaaccg gctggggcga
    19321 atccgttctc gctggatgat tttgaatttt cgccaatgaa agcgttgctg gatgatggtc
    19381 cacacgaacc gctgtttacg ccaattgttg aacctgtaca gcagccgcaa caaccggttg
    19441 caccgcagca gcagtatcag cagccacaac agccggttgc gccacagccg cagtatcagc
    19501 agccacaaca gcaggttgcg ccgcagccac aatatcagca gccgcaacaa ccggttgcgc
    19561 cacagcagca atatcagcag ccgcaacaac cagttgcgcc gcagccgcag tatcagcagc
    19621 cgcaacaacc agttgcgccg cagccgcagt atcaacagcc gcaacaaccg gttgcaccgc
    19681 agccgcagta tcagcagcca caacagccag ttgcgccaca gccgcaggat accctgcttc
    19741 atccgctgtt gatgcgtaat ggcgacagcc gtccgttgca caaaccgacg acgccgctgc
    19801 cttctctgga tttgctgaca ccgccgccga gcgaagtgga gccggtagat acctttgcgc
    19861 ttgaacaaat ggcgcgtctg gtggaagcgc gtctggctga tttccgtatt aaagccgatg
    19921 tcgttaatta ctctccgggg ccagttatca ctcgctttga acttaacctg gctcctggcg
    19981 taaaagcggc gcgcatttct aacttgtcac gggaccttgc ccgttcactt tcgacggtgg
    20041 cggtgcgtgt cgttgaagtt attcctggca aaccctatgt aggtctggag ttaccaaata
    20101 aaaaacgaca aaccgtttat ctgcgcgaag ttttggataa cgccaaattc cgcgataatc
    20161 cgtcgccatt aaccgtggtg ctgggtaaag atatcgccgg tgagccggtg gttgccgatc
    20221 tggcgaaaat gccgcacttg ttggttgcgg ggactaccgg ttccggtaaa tctgtcggtg
    20281 tgaacgcgat gatcctgagc atgctttata aagcacagcc agaagatgtg cgtttcatca
    20341 tgatcgaccc gaaaatgctg gagctttcgg tttatgaagg cattccgcac ctgttaacgg
    20401 aagtcgttac tgatatgaaa gatgccgcca acgcgctgct ctggtgtgtt aacgagatgg
    20461 agcggcggta taaactgatg tctgcgctgg gtgtgcgtaa tctggcgggt tataacgaaa
    20521 aaattgctga agccgatcgc atgatgcgtc cgattccaga cccgtactgg aagccgggtg
    20581 acagtatgga tgcccagcat ccggtgctga aaaaagaacc atacattgtc gtgttggttg
    20641 acgaatttgc cgacctgatg atgacggtag gtaaaaaagt ggaagagctg atagcacgtc
    20701 tggcgcaaaa agcccgtgcc gcgggtatcc acctcgtact ggcaactcag cgtccatcgg
    20761 ttgatgttat tactggtctg attaaagcga atattccgac ccgtatcgcc tttaccgtat
    20821 ccagtaagat tgactcacgt accattcttg atcaggctgg cgcggaatca ctgctgggta
    20881 tgggggatat gctctactct gggccgaact ccacgttgcc ggtacgtgtc catggtgctt
    20941 ttgttcgcga tcaggaagtt catgccgtgg tgcaggactg gaaagcgcgt ggtcgcccac
    21001 agtatgttga tggcatcacc tccgacagcg aaagcgaagg tggtgcgggt ggtttcgatg
    21061 gcgctgaaga actggatccg ttgttcgatc aggcggtgca gtttgtcact gaaaaacgca
    21121 aagcgtcaat ttctggcgta cagcgtcagt tccgcattgg ttataaccgt gcagcgcgta
    21181 ttatcgaaca gatggaagcg caggggattg tcagcgaaca ggggcacaac ggtaatcgtg
    21241 aagtgctggc cccaccgccg tttgactaat taatgcattg ccggataagg cgcggtagcg
    21301 tcgcatccgg cactctatca actgaaaatt cagtattttc ttctttcctc aagctgatta
    21361 ttagcctgga atagagagta gagggaactc ccgatcggga gtgacgtaat ttgaggaata
    21421 atgatgaaaa aaattgccat cacctgtgca ttactctcaa gcttagtagc aagcagcgtt
    21481 tgggctgatg ccgcaagcga tctgaaaagc cgcctggata aagtcagcag cttccacgcc
    21541 agcttcacac aaaaagtgac tgacggtagc ggcgcggcgg tgcaggaagg tcagggcgat
    21601 ctgtgggtga aacgtccaaa cttattcaac tggcatatga cacaacctga tgaaagcatt
    21661 ctggtttctg acggtaaaac actgtggttc tataacccgt tcgttgagca agctacggca
    21721 acctggctga aagatgccac cggtaatacg ccgtttatgc tgattgcccg caaccagtcc
    21781 agcgactggc agcagtacaa tatcaaacag aatggcgatg actttgtcct gacgccgaaa
    21841 gccagcaatg gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg cacaatccat
    21901 cagtttagcg cggtggagca ggacgatcag cgcagcagtt atcagctgaa atcccagcaa
    21961 aatggggctg tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt cacggtagat
    22021 gatcaacgta agtagaggca cctgagtgag caatctgtcg ctcgattttt cggataatac
    22081 ttttcaacct ctggccgcgc gtatgcggcc agaaaattta gcacagtata tcggtcagca
    22141 acatttgctg gctgcgggga agccgttgcc gcgcgctatc gaagccgggc atttgcattc
    22201 tatgatcctc tgggggccac cgggtaccgg caaaacaacc ctcgctgaag tgattgcccg
    22261 ctatgcgaac gctgatgtgg aacgtatttc tgccgtcacc tctggcgtga aagagattcg
    22321 cgaggcgatc gagcgcgccc ggcaaaaccg caatgcaggt cgccgcacta ttctttttgt
    22381 tgacgaagtt caccgtttca acaaaagcca gcaggatgca tttctgccac atattgaaga
    22441 cggcaccatc acttttattg gcgcaaccac tgaaaacccg tcgtttgagc ttaattcggc
    22501 actgctttcc cgtgcccgtg tttatctgtt gaaatccctg agtacagagg atattgagca
    22561 agtactaact caggcgatgg aagacaaaac ccgtggctat ggtggtcagg atattgttct
    22621 gccagatgat acacgacgtg ccattgcaga actggtgaat ggcgacgcgc gccgggcgtt
    22681 aaatacgctg gaaatgatgg cggatatggc cgaagtctat gatagcggta agcgggtcct
    22741 gaagcctgaa ttactgaccg aaatcgccgg tgaacgtagc gcccgctttg ataacaaagg
    22801 cgatcgcttt tacgatctaa tttccgcact gcataagtcg gtacgtggta gcgcacccga
    22861 tgcggcgctg tactggtatg cgcgaattat taccgctggt ggcgatccgt tatatgtcgc
    22921 gcgtcgctgt ctggcgattg cgtctgaaga cgtcggtaat gccgatccac gggcgatgca
    22981 ggtggcaatt gcggcctggg attgctttac tcgcgttggc ccggcggaag gtgaacgcgc
    23041 cattgctcag gcgattgttt acctggcctg cgcgccaaaa agcaacgctg tctacactgc
    23101 gtttaaagcc gcgctggccg atgctcgcga acgcccggat tatgacgtgc cggttcattt
    23161 gcgtaatgcg ccgacgaaat taatgaagga aatgggctac gggcaggaat atcgttacgc
    23221 tcatgatgaa gcaaacgctt atgctgccgg tgaggtttac ttcccgccgg aaatagcaca
    23281 aacacgctat tatttcccga caaacagggg ccttgaaggc aagattggcg aaaagctcgc
    23341 ctggctggct gaacaggatc aaaatagccc cataaaacgc taccgttaat gttatcgttg
    23401 cggtaatgtt gttactgtat ccctgtggtc gcaggctgtg gccacatctc ccatttaatt
    23461 cgataagcac aggataagca tgctcgatcc caatctgctg cgtaatgagc cagacgcagt
    23521 cgctgaaaaa ctggcacgcc ggggctttaa gctggatgta gataagctgg gcgctcttga
    23581 agagcgtcgt aaagtattgc aggtcaaaac ggaaaacctg caagcggagc gtaactcccg
    23641 atcgaaatcc attggccagg cgaaagcgcg cggggaagat atcgagcctt tacgtctgga
    23701 agtgaacaaa ctgggcgaag agctggatgc agcaaaagcc gagctggatg ctttacaggc
    23761 tgaaattcgc gatatcgcgc tgaccatccc taacctgcct gcagatgaag tgccggtagg
    23821 taaagacgaa aatgacaacg ttgaagtcag ccgctggggt accccgcgtg agtttgactt
    23881 tgaagttcgt gaccacgtga cgctgggtga aatgcactct ggcctcgact ttgcagctgc
    23941 agttaagctg actggttccc gctttgtggt aatgaaaggg cagattgctc gcatgcaccg
    24001 cgcactgtcg cagtttatgc tggatctgca taccgaacag catggctaca gtgagaacta
    24061 tgttccgtac ctggttaacc aggacacgct gtacggtacg ggtcagctgc cgaaatttgc
    24121 tggcgatctg ttccatactc gtccgctgga agaagaagca gacaccagta actatgcgct
    24181 gatcccaacg gcagaagttc cgctgactaa cctggtgcgc ggtgaaatca tcgatgaaga
    24241 tgatctgcca attaagatga ccgcccacac cccatgcttc cgttctgaag ccggttcata
    24301 tggtcgtgac acccgtggtc tgatccgtat gcaccagttc gacaaagttg aaatggtgca
    24361 gattgtgcgc ccagaagact caatggcggc gctggaagag atgactggtc atgcagaaaa
    24421 agtcctgcag ttgctgggcc tgccgtaccg taaaatcatc ctttgcactg gcgacatggg
    24481 ctttggcgct tgcaaaactt acgacttgga agtatggatc ccggcacaga acacctaccg
    24541 tgagatctct tcctgctcca acgtttggga tttccaggca cgtcgtatgc aggcacgttg
    24601 ccgcagcaag tcggacaaga aaacccgtct ggttcatacc ctgaacggtt ctggtctggc
    24661 tgttggtcgt acgctggttg cagtaatgga aaactatcag caggctgatg gtcgtattga
    24721 agtaccagaa gttctgcgtc cgtatatgaa cggactggaa tatattggct aatacccaat
    24781 ttttctgaat ctaaaaagcg cctttagggc gctttttttg tctccctttg ataccgaaca
    24841 ataattactc ctcacttaca cgtaatacta ctttcgagtg aaaatctacc tatctctttg
    24901 attttcaaat tattcgatgt atacaagcct atatagcgag ctgctataga aataattaca
    24961 caatacggtt tgttactgga atcaatcgtg atcaagcttg agtgagccat tatgaaaacg
    25021 aaaatccctg atgcggtatt ggctgctgag gtgagtcgcc gtggtttggt aaaaacgaca
    25081 gcgatcggcg gcctggcaat ggccagcagc gcattaacat taccttttag tcggattgcg
    25141 cacgctgtcg atagcgccat tccaacaaaa tcagacgaaa aggttatctg gagcgcctgt
    25201 acagttaact gtggtagtcg ctgcccgcta cgtatgcacg tcgtggacgg tgaaatcaaa
    25261 tatgtcgaaa cggacaatac cggcgatgac aattacgacg gcctgcacca ggttcgcgcc
    25321 tgcctgcgtg ggcgttccat gcgtcgccgt gtctacaatc cggaccgcct gaaatatccg
    25381 atgaaacgag tcggggcgcg cggtgaaggc aaattcgagc gcattagctg ggaagaagcc
    25441 tacgacatca tcgcgaccaa tatgcagcgc ctgatcaaag agtacggcaa cgagtctatc
    25501 tatctgaact atggcaccgg tacgctgggc ggcaccatga cccgctcctg gccgccggga
    25561 aataccctgg tcgcgcgcct gatgaactgc tgcggcggtt atcttaacca ttacggtgac
    25621 tactcttccg cgcaaattgc ggaaggtttg aaccatacct acggcggctg ggcagatggt
    25681 aacagcccgt cggatatcga aaacagtaaa ttggtagtgc tgtttggtaa taacccaggc
    25741 gaaacgcgaa tgagtggcgg tggggtgact tactatcttg aacaggcacg ccagaaatct
    25801 aatgctcgca tgatcatcat cgatccgcgc tataccgaca ccggtgccgg gcgcgaagat
    25861 gagtggatcc ctattcgtcc aggaacagat gccgcactgg ttaacggtct ggcgtacgtc
    25921 atgatcactg aaaacctggt ggatcaggca ttcctcgata aatattgcgt tggctatgac
    25981 gagaaaaccc tgcccgccag tgcgccgaaa aatggtcatt ataaagctta tattctgggt
    26041 gaagggccag atggcgtggc taaaacaccg gaatgggcct cgcaaatcac cggtgttccg
    26101 gcagacaaaa tcatcaaact ggctcgtgaa atcggcagta ccaaaccggc gtttatcagc
    26161 cagggatggg gcccgcagcg tcacgctaac ggtgaaatcg caacccgtgc tatctcgatg
    26221 ctggcgattc tgaccggtaa cgttggtatt aatggaggga acagcggcgc gcgtgaaggt
    26281 tcatacagct taccgtttgt ccgtatgccg accttggaaa acccgatcca gaccagcatt
    26341 tcgatgttta tgtggaccga tgccattgaa cgtggcccgg aaatgacggc gctgcgtgat
    26401 ggtgtgcgcg ggaaagataa gctggatgtg ccgatcaaaa tgatctggaa ctatgccggt
    26461 aactgcctga ttaaccagca ttctgaaatc aactgtaccc atgaaatcct ccaggatgat
    26521 aagaagtgcg agctgattgt ggttatcgac tgccacatga cctcatcggc gaaatatgct
    26581 gacatcctgc tgcctgactg caccgcttcc gaacagatgg actttgcact ggatgcatcc
    26641 tgcgggaata tgtcttacgt gattttcaac gatcaggtga ttaaaccgcg ctttgaatgt
    26701 aagaccatct atgaaatgac cagcgaactg gcaaaacgtc ttggcgttga gcaacagttt
    26761 actgaaggcc gtacccagga agagtggatg cggcatctgt atgcccagtc gcgggaagcg
    26821 attcctgaac tgccaacgtt tgaagagttc cgcaagcagg ggatctttaa aaagcgcgac
    26881 ccacaagggc atcacgttgc ttataaagcc ttccgtgaag atccgcaggc aaacccactg
    26941 actacgccat cgggcaaaat tgagatttat tcgcaggcgc tggctgacat tgccgctacc
    27001 tgggaattgc cagaaggcga tgtgatcgat ccactgccga tttacacgcc gggctttgaa
    27061 agttatcagg atccgctgaa caaacagtat ccgctgcagc ttacaggttt ccactataaa
    27121 tctcgcgttc actcaactta cggcaacgtt gatgtgctga aagcggcttg ccgtcaggaa
    27181 atgtggatca acccgcttga tgcccaaaaa cgcggtatcc acaacggcga taaagtcagg
    27241 atctttaacg atcgtggtga ggttcatatt gaggcgaaag tgacgccacg aatgatgccg
    27301 ggcgtggtcg cactgggtga aggcgcctgg tatgacccgg atgcaaaacg tgtcgataag
    27361 ggcggttgta tcaacgtact gaccactcaa cgtccgtctc ctctcgctaa ggggaatccg
    27421 tcacatacaa accttgttca ggttgaaaag gtgtaaggag taaccgatga caacccagta
    27481 tggatttttt attgattcca gccgttgcac cggttgcaaa acctgcgagc tggcttgtaa
    27541 agactacaaa gatttgacgc cagaagtcag tttccgccgc atttatgaat atgctggcgg
    27601 cgactggcag gaagataacg gtgtctggca ccagaacgtg tttgcctact atctgtcgat
    27661 ttcgtgcaac cactgcgaag atccggcttg tactaaagtc tgcccgagcg gtgcgatgca
    27721 taaacgcgaa gatggttttg ttgtggtcga tgaagatgta tgcattggct gccgttactg
    27781 tcatatggct tgcccgtatg gcgcaccgca atataacgaa acgaaaggcc atatgaccaa
    27841 atgcgatggt tgttatgacc gtgttgcgga gggtaaaaag ccgatctgtg ttgaatcttg
    27901 cccgctgcgg gcgctggatt tcggccctat cgacgagctg cgtaaaaaac atggcgatct
    27961 ggcggccgtt gcgccgttgc cgcgagctca ctttaccaaa ccgaatattg tgatcaaacc
    28021 caatgccaat agccgcccga ccggggatac cactggctat ctggcaaacc cgaaggaggt
    28081 gtgagatggg aagtggatgg catgaatggc cgctgatgat cttcacggtc ttcgggcaat
    28141 gtgtggcggg tggttttatc gttctggctt tggcgctgct caaaggcgac ctgcgagcag
    28201 aagcccagca gcgtgttatc gcctgcatgt ttggcttatg ggtactgatg ggcattggct
    28261 ttatcgcctc tatgctccat cttggttcac cgatgcgcgc ctttaactcg ctcaaccggg
    28321 taggggcttc agcactcagt aacgaaatcg ccagcggttc gatctttttt gctgtaggcg
    28381 gcatcggctg gttgctggca atgctgaaaa agctg
//
LOCUS       DBKEKI010000046        28354 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.046, whole genome shotgun sequence.
ACCESSION   DBKEKI010000046 DBKEKI010000000
VERSION     DBKEKI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.046"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..391)
                     /locus_tag="ACUQPX_001924"
     CDS             complement(<1..391)
                     /locus_tag="ACUQPX_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HHV9056647.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPY"
     gene            complement(462..2576)
                     /gene="fusA"
                     /locus_tag="ACUQPX_001925"
     CDS             complement(462..2576)
                     /gene="fusA"
                     /locus_tag="ACUQPX_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HHV9056648.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2673..3143)
                     /gene="rpsG"
                     /locus_tag="ACUQPX_001926"
     CDS             complement(2673..3143)
                     /gene="rpsG"
                     /locus_tag="ACUQPX_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HHV9056649.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3240..3614)
                     /gene="rpsL"
                     /locus_tag="ACUQPX_001927"
     CDS             complement(3240..3614)
                     /gene="rpsL"
                     /locus_tag="ACUQPX_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HHV9056650.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3740..4027)
                     /gene="tusB"
                     /locus_tag="ACUQPX_001928"
     CDS             complement(3740..4027)
                     /gene="tusB"
                     /locus_tag="ACUQPX_001928"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HHV9056651.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4035..4394)
                     /gene="tusC"
                     /locus_tag="ACUQPX_001929"
     CDS             complement(4035..4394)
                     /gene="tusC"
                     /locus_tag="ACUQPX_001929"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HHV9056652.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4394..4780)
                     /gene="tusD"
                     /locus_tag="ACUQPX_001930"
     CDS             complement(4394..4780)
                     /gene="tusD"
                     /locus_tag="ACUQPX_001930"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HHV9056653.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4780..5502)
                     /locus_tag="ACUQPX_001931"
     CDS             complement(4780..5502)
                     /locus_tag="ACUQPX_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9056654.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5669..6481)
                     /gene="fkpA"
                     /locus_tag="ACUQPX_001932"
     CDS             complement(5669..6481)
                     /gene="fkpA"
                     /locus_tag="ACUQPX_001932"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HHV9056655.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6702..6920
                     /locus_tag="ACUQPX_001933"
     CDS             6702..6920
                     /locus_tag="ACUQPX_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HHV9056656.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6969..7559)
                     /gene="slyD"
                     /locus_tag="ACUQPX_001934"
     CDS             complement(6969..7559)
                     /gene="slyD"
                     /locus_tag="ACUQPX_001934"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HHV9056657.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7654..7854)
                     /locus_tag="ACUQPX_001935"
     CDS             complement(7654..7854)
                     /locus_tag="ACUQPX_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HHV9056658.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7864..9669)
                     /gene="kefB"
                     /locus_tag="ACUQPX_001936"
     CDS             complement(7864..9669)
                     /gene="kefB"
                     /locus_tag="ACUQPX_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HHV9056659.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKTQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9669..10220)
                     /gene="kefG"
                     /locus_tag="ACUQPX_001937"
     CDS             complement(9669..10220)
                     /gene="kefG"
                     /locus_tag="ACUQPX_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HHV9056660.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10351..12264
                     /locus_tag="ACUQPX_001938"
     CDS             10351..12264
                     /locus_tag="ACUQPX_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHV9056661.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12264..13286
                     /locus_tag="ACUQPX_001939"
     CDS             12264..13286
                     /locus_tag="ACUQPX_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HHV9056662.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13280..13498
                     /locus_tag="ACUQPX_001940"
     CDS             13280..13498
                     /locus_tag="ACUQPX_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HHV9056663.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13552..14421
                     /locus_tag="ACUQPX_001941"
     CDS             13552..14421
                     /locus_tag="ACUQPX_001941"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HHV9056664.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14476..14880)
                     /locus_tag="ACUQPX_001942"
     CDS             complement(14476..14880)
                     /locus_tag="ACUQPX_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HHV9056665.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15182..15814
                     /gene="crp"
                     /locus_tag="ACUQPX_001943"
     CDS             15182..15814
                     /gene="crp"
                     /locus_tag="ACUQPX_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HHV9056666.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15865..17955
                     /locus_tag="ACUQPX_001944"
     CDS             15865..17955
                     /locus_tag="ACUQPX_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HHV9056667.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVILSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18022..19242)
                     /gene="argD"
                     /locus_tag="ACUQPX_001945"
                     /gene_synonym="dapC"
     CDS             complement(18022..19242)
                     /gene="argD"
                     /locus_tag="ACUQPX_001945"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HHV9056668.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19328..19891)
                     /gene="pabA"
                     /locus_tag="ACUQPX_001946"
     CDS             complement(19328..19891)
                     /gene="pabA"
                     /locus_tag="ACUQPX_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HHV9056669.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19923..20525)
                     /locus_tag="ACUQPX_001947"
     CDS             complement(19923..20525)
                     /locus_tag="ACUQPX_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HHV9056670.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20515..20682)
                     /locus_tag="ACUQPX_001948"
     CDS             complement(20515..20682)
                     /locus_tag="ACUQPX_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HHV9056671.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20787..21359)
                     /gene="ppiA"
                     /locus_tag="ACUQPX_001949"
                     /gene_synonym="rotA"
     CDS             complement(20787..21359)
                     /gene="ppiA"
                     /locus_tag="ACUQPX_001949"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HHV9056672.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21630..22811
                     /gene="tsgA"
                     /locus_tag="ACUQPX_001950"
     CDS             21630..22811
                     /gene="tsgA"
                     /locus_tag="ACUQPX_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HHV9056673.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23073..25616
                     /gene="nirB"
                     /locus_tag="ACUQPX_001951"
     CDS             23073..25616
                     /gene="nirB"
                     /locus_tag="ACUQPX_001951"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HHV9056674.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25613..25939
                     /gene="nirD"
                     /locus_tag="ACUQPX_001952"
     CDS             25613..25939
                     /gene="nirD"
                     /locus_tag="ACUQPX_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HHV9056675.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26065..26871
                     /gene="nirC"
                     /locus_tag="ACUQPX_001953"
     CDS             26065..26871
                     /gene="nirC"
                     /locus_tag="ACUQPX_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HHV9056676.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26890..28263
                     /gene="cysG"
                     /locus_tag="ACUQPX_001954"
     CDS             26890..28263
                     /gene="cysG"
                     /locus_tag="ACUQPX_001954"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HHV9056677.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLQEAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6865 a         7601 c         7162 g         6726 t
ORIGIN      
        1 tgtacggaac gcctacctga cgacccagca ggatgtgctc acgagtctgc ggcatcgggc
       61 cgtcagtcgc agcaactacc aggatcgcgc cgtccatctg agcagcaccg gtgatcatgt
      121 ttttaacata gtcggcgtgc cccgggcagt ctacgtgtgc gtagtgacgg gtcggggtgt
      181 catattcaac gtgagaagtg ttgatggtga taccacgagc tttttcttcc ggcgcgttat
      241 cgatctggtc gaatgcacga gcagcaccgc cgtaggtttt agccagtacg gtggtgattg
      301 cagcggtcag agtagtttta ccgtggtcaa cgtggccgat agtaccaacg ttaacgtgcg
      361 gttttgtacg ttcaaatttt tctttagaca cggctatatt ccttactata gtgctctccc
      421 cttcaggaga gagcacggga ctttggtatt aacccttagg cttatttacc acgggcttca
      481 attacggcct gagcaacgtt actcggcgct tcatcatact tcaggaattc catagtgtat
      541 gatgcacgac ctttggtcag agaacgcagc tgagttgcgt atccgaacat ttcagacagc
      601 ggtacttcag cgtggatctt aacgccagta acttcagatt cctgaccttt gagcatacca
      661 cgacgacggc tcaagtcacc gataacgtca ccggtgttct cttccggagt ttctacttca
      721 accttcatga tcggctcaag cagaactggt ttcgctttct taaagccttc tttaaaggcg
      781 atagaagcag ccagtttaaa cgccagttca gaggagtcaa cgtcatggta agaaccgaag
      841 tgcagacgaa tacccatgtc tactaccggg tagcctgcca gcggacctgc tttcagctgt
      901 tcctggatac ctttatcaac ggccgggatg tattcgccag ggattacacc acctttaatg
      961 tcgttgatga actcgtagcc tttcgggttt gaacccggct ccagcgggta catgtcgata
     1021 acaacatgac catactgacc acgaccacca gactgtttcg cgtgtttacc ttcaacatcg
     1081 gtaactttct ggcggatagt ttcacggtaa gcaacctgcg gtttacctac gttcgcttca
     1141 acgttgaatt cacgcttcat acggtcaacg atgatgtcga ggtgcagttc gcccataccc
     1201 gcgatgatgg tctggttaga ttcttcgtca gtccatacac ggaaagacgg gtcttcttta
     1261 gccagacggc ccagagccag acccattttt tcctggtcag ctttggtttt cggttcaact
     1321 gcgatggaga ttaccggctc agggaattcc atacgttcca gaatgatcgg cgcatccggg
     1381 tcacacaggg tgtcaccagt ggttacgtct ttcagaccga tagcagcagc gatgtcgccc
     1441 gcgcgaactt ctttgatctc ttcacgtttg ttagcgtgca tctgaacgat acgaccgaaa
     1501 cgctcacgtg cagctttcac ggagttcagt acggtatcac cagagttaac cacaccggag
     1561 taaacacgga agaaggtcag gttaccaaca aacgggtcgg tagcgatttt gaacgccagt
     1621 gcagagaacg gctcgtcatc acttgcgtga cgttcagccg gagtgtcttt accgtcgtcc
     1681 aggataccgt tgatcgcagg tacgtcaacc ggggatggca ggtaatcaat taccgcatcc
     1741 agcatcgcct gaacaccttt gttcttgaac gcagaaccac aggttaccag gatgatttcg
     1801 ttgttcagaa cgcgctgacg cagagcacct ttgatttctg cttcagtcag ttcttcacca
     1861 cccaggtatt tttccatcag ctcttcagaa gcttcagctg cggattcgat caggttctgg
     1921 tgccattcgt tagccagttc aaccatgtct gccgggatat cttcgtattc gaaggttacg
     1981 ccctggtcag cgtcgttcca gttgatagct ttcattttca ccaggtcaac aacaccggtg
     2041 aaatgttctt cagcaccaat cgccagctgc agcggaaccg ggttcgcgcc cagacgggtt
     2101 ttgatctggt taacaacttt caggaagttc gcacccatgc ggtccatttt gttaacgaac
     2161 gcaatgcgcg gaactttata tttgtttgcc tgacgccata cggtttcaga ctgcggctga
     2221 acaccaccaa ctgcgcagta aaccattacc gcaccatcga gaacacgcat ggaacgttct
     2281 acttcgattg tgaagtcaac gtgccccggg gtgtcgatga tgttgatgcg atgcggctca
     2341 tactgcttag ccataccaga ccagaatgca gtagtcgcag cggaagtgat ggtaatacca
     2401 cgttcctgct cctgctccat ccagtccatg gttgcagcgc cgtcatgaac ttcaccgatt
     2461 ttatggttta caccggtgta gaacagaata cgttcggtag tagtggtttt accggcgtcg
     2521 atgtgcgcac tgataccgat gttacggtag cgtgcgatgg gtgttgtacg agccatttgt
     2581 ttcctcgttt atcttttagg cgttcaattt aagtagccca aagcgggctg cttactggaa
     2641 gcgcccgcct ggtgactaaa actccgaagg gattaccaac ggtagtgtgc gaacgccttg
     2701 ttggcttcgg ccatacggtg aacgtcttca cgtttcttaa ctgcagtacc tttgttttct
     2761 gcagcatcag aaagttcgtt cgccaggcgc agagccatgg atttatcacc gcgtttacga
     2821 gcagcttcaa cgatccaacg cattgccaga gcattacgac gaaccggacg gacttcaact
     2881 ggtacctgat aagtagaacc accaacgcgg cgagacttaa cttctacagt cgggcgcacg
     2941 ttttcgagag ctacttcgaa tgcttccagt tcagatttac cagagcgctg agccagggtc
     3001 tccagcgcgc tgtatacgat agattcagca gtagattttt taccatctac catcaggata
     3061 tttacaaatt tagccagcag ttctgatccg aacttcggat ccggcagaat tttacgctga
     3121 ccaatgacgc gacgacgtgg catggaaata ctccgttgtt aattcaggat tgtccaaaac
     3181 tctacgagtt tagtttgaca tttaagttaa aacgtttggc cttacttaac ggagaaccat
     3241 taagccttag gacgcttcac gccatacttg gaacgagcct gcttacggtc tttaacgccg
     3301 gagcagtcaa gcgcaccacg tacggtgtgg taacgaacac ccgggaggtc tttaacacga
     3361 ccgccacgga tcaggatcac ggagtgctcc tgcaggttgt gaccttcacc accgatgtag
     3421 gaagtcactt cgaaaccgtt agtcagacga acacggcata ctttacgcag cgcggagttc
     3481 ggttttttag gagtggtagt atatacacga gtacatacgc cacgtttttg cgggcatgct
     3541 tccagcgcag gcacgttgct tttcgcaact ttgcgagcac gtggtttgcg taccagctgg
     3601 ttaactgttg ccattaaata gctcctggtt ttagcttttg cttcgtaaac acgtaataaa
     3661 acgtcctcac acaatatgag gacgccgaat tttagggcga tgccgaaaag gtgtcaagaa
     3721 atatacaacg atcccgccat caccaggcca tctggctgga gtgcttaacc gtaagtctga
     3781 cgaaatcagt atagtcaatg ggaatgatgt cgttcaaaat ttgaccagtc aaaccgcggg
     3841 caataaggtc ttcgttcagg gcatagacct taatgggggc attacgcaga ctttcaaggt
     3901 agcggttacc gtcaactgcg gcagttacgc catcttgcaa taatagcagt tcgtctcctt
     3961 cactgagcag acgcagcagc gcagcaaaat ccgtcagcca gggtgaacga tgtaacgtgt
     4021 gcagcataaa cgcctcaaaa cctcaaaata acatcgtagt tggcgagttc gcggcgtaag
     4081 gcatctgctt cgagcggcgt ggcttcgaca acaaagggtg tctgcggatc taacccgcgt
     4141 tcgcgcaatg aagccgcaca aacccagcac tgttcaatgt cgtacagact caacaattta
     4201 aaagtggcaa tgtaatcacg cgccagcact gcatcgggct tttgtcctga cagcagctga
     4261 aaaacgccat cagcaataaa gaagacggcc agatcgtcag ttaatgcgga agttgccagt
     4321 aaagcatcta aaccttcccg accagcagct gtaccatgag gtgcagtaga aaaaacaaac
     4381 gcaattcgtt tcatcagaac tgtaccaccc tgtcacaggt cagcgaggct tccgccagcg
     4441 cgccaagtcc gcttaaggta aatcccggct gaaggtttga cgaagccagc cccagccttc
     4501 ctgcttcagt ttcatcgacg ataccgcggc gtaacgctgc cgctacgcag atattcagcg
     4561 ccacaccatg ttgcgcattc aactgttgcc agccgcgcac aaggtcaaat tcgtcgcttg
     4621 ccggtgaggt cagttggtta gcgttataga ccccttcccg atagaaaaag acgctgctta
     4681 acttatggcc ttctgctatc agcgcctgcg caaactgaaa agcactactc gcctgttgcg
     4741 taccgtatgc tggcccggtc accacgatgg caaaacgcat tacttatctt gcccctggaa
     4801 atcaccgctc ttgaactggc ggatgtagag atagacagtg tgtttggaga tgttcaggcg
     4861 gtcagcaacc tggttgatcg catctttaat atcgaagatc cctttctcgt agagattcag
     4921 cacgatctga cgatttttgg cgttattaga aacattgcga tcggcattca cttcttcgat
     4981 ggtgaactcc agcgtttggg taaccagatc ttcaacagaa gaggcaaagt tgacgcttga
     5041 accgacatcc ggggtttctg gcggcacaaa agtgctcata atctgcgaga agggaacatc
     5101 aagattcata ttgatgcaca gcagaccaat tacacgctgt tcgcggttac gaatcgcgat
     5161 agtcagggac ttcattaata cgccgctttt ggcgcgagta aagtagcatt tagaaacgct
     5221 gctatccgct cccgtcatat cgtgcagcat acgtagcgcc aggtcagtaa ttggcgaacc
     5281 aatcttccgg cctgtatgtt caccgttagc aatgcgaatg gctgaacatt ttagatcctg
     5341 caaagagtgc aaaacgattt cacagtggga gccaataagc atcgctaacc cgtccaccac
     5401 cgcttcgtag gatttcagaa tatcaaaatc ggtctggtcg aaaggacgtt gatccagtaa
     5461 atccaactca ctggtttcgt tggttaaaag cgacctggac atgaaaaaaa acactccttt
     5521 tcaggagcct gtcgttaact tttcagggca ggctcattaa tgatgcgggt aactaaatta
     5581 atacagcgga ggttccgctt tccagcacta attatatccg gcctgtaata aaaaaaccgc
     5641 cgcctggtca ggcggcggtt cttaatgctt attttttagc agaatctgcg gctttcgcat
     5701 cagcttccgg ctttgcatca gccttcggcg ctggtttcac atccagcagc tctacgtcaa
     5761 acactagggt agaattcggt gggatccccg gaacacccgc tttgccgtaa gccagttctg
     5821 gtggaataac cagtttgatc ttaccgcctt tcttgatgtt cttcagacct tctgtccaac
     5881 ccgggataac accgtccaga cggaaagaaa gcggttcacc acgggtgtaa gagttgtcga
     5941 actctttacc gtcgatcagc gtacctttgt agttcactac aacagtatcg ctgtctttcg
     6001 gtgcttcgcc tttaccggct tctactacct gataaaccag accagttgaa gaggttttca
     6061 cacctttctc tttggcaaat ttctcgcggt actctttacc ttttgcttcg ttatcagccg
     6121 cgtctttttc catcttcgcc tgagcagaag acttcacgcg agcttcgaat gcttgcagag
     6181 tctgttcgat ctcttggtcg gagagtttgc tcttatcagc aaatgcatcc tgaacaccag
     6241 cgatcagctg atctttatcc agtttgatgc ccagtttttc ttgttcttta agagagtttt
     6301 ccatgtaacg acccagcgaa gcacccagtg cataagctga tttctgatcg tcatttttga
     6361 acgctgcttt gctgtcagca gttgtagcag gttttgcagc ttcagcagca aaagtgattg
     6421 gtgcatgcag ggcaacggcc attgtggtcg ccagtagcgt tactttaaac agtgatttca
     6481 tccatatctc caggatcggg gcatctcacc ccaaggttaa ctattatcag aagggtacta
     6541 taaagcgttg tcgaacaaat ctacatacag acacgcccta ttatcatcta ttttcagact
     6601 ctttttgttt aaattagttt cgatgaccgc gaaatgagtg ctgtctcggg cagcaaagtt
     6661 aagtagaatc cgcggcgacc attcgacaaa agaggtgaat catgcaggat ttatcattgg
     6721 aagcacgcct ggcagagctg gagagccgac tggcttttca ggaaatcacc attgaagaac
     6781 tgaacgtcac ggtgaccgct catgaaatgg agatggcgaa actgcgcgat catctgcgtc
     6841 tgctgaccga gaagttaaaa gccagccagc cgtcgaacat cgcgtcgcag gctgaagaaa
     6901 cgccaccgcc acattattga ggcgtaaaaa aagcggggat tccccgcttt tttgtcacct
     6961 tttcggtatt agtggcaacc acaaccgccg ttgcctttac cgccacagca gccttcgcca
     7021 ccgtgttcat gaccgtgatc gtggccatga ccgccgcagc aaccgtcgtg gtcgtgatcg
     7081 tggtgatgat cgtgcgcgcc gtgaacgtgg ccatgagcca gttcttcttc ggtcgcttcg
     7141 cgaatcgcca caacttcaac gttgaatttc aggttctgac cggccagcat gtggttacca
     7201 tcaaccacta cgtgatcgtc ttcaaccgca gtgatttcaa ccggtaccgg accctggtca
     7261 gtttcagcca ggaaacgcat acctacctgc agttcatcaa cgcccataaa tacgtcttta
     7321 ggaacacgtt gcactaggtt ttcgtcgtac tgaccgtaag cgtcgttcgc gccaacagcg
     7381 acatcaaatt tgtcgccaac ttcatgacct tccagcgccg tttccaggcc agagatcagg
     7441 gaaccgtgac catgcaggta gtccagcggc gcactcaccg gagactcatc aaccaacaca
     7501 ccgtcttctg tacgtacctg ataggccagg ctgaccacca ggtcttttgc tactttcatg
     7561 atatctcctg agcatgggaa gaatagtggc gcagattgta gcggaattct gcagccgtgt
     7621 actcactagc ttaaaaaaac tcggctcata tcgctagtcc ggatgaaaaa tcccgatcac
     7681 ttgctcatct ttgcgaacgt gatcgcgggc ttctttgtct gcttctcgca tctgatgtcc
     7741 gcacttaaca cattcaacaa tatcaatatt attttcgcgc cacatcgcca ttgaatcctg
     7801 cgcctgacag gccgggcatt ttgcgcccgc tataaaacgt tttcggattg ccatccttac
     7861 cctctactca aattcatccc agccgtccag ctggcgtcgt tcttgttgca tttcacgctg
     7921 gaaaatctct tccagttcgc gtcgggcttc cctggcgcga gaaatttgta cggtatcagc
     7981 atgcatcggg attaactccc gcagcattcg catatccagg cggcgaaaat gcagttgcgc
     8041 ccgctgtgcc tgatgcggat gcatgccaag cgtgaccagc gtcttgcgcc ccagctctaa
     8101 cgcactggag aatgtttcac gggaaaactg cgtcacccct gcctgtaata actcatgcgc
     8161 ttccacacgt ccgcgcgctc gcgcaagaat atgcaaatgc ggaaagtgct gctggcatat
     8221 ttccaccagc ttcatggtgt cttccggctc gttacaggta atgacgatag actcagcggc
     8281 ctctgcaccc gcagaacgta aaagatcgac ctgcgtggcg tcgccgtaat aaactttgta
     8341 gccatattta cgcatcaggt taacggcgct gatatcccgc tccagcacgg taatgcgcat
     8401 tttatttgcc atcagcaaac gaccaatcac ctgaccaaaa cgcccgaagc ccacgacaat
     8461 gacctgggtt ttatcatcgt tgacccacgg tttttcgtct tcttcttccg gtccgttaaa
     8521 ctggcgggat agccatttat ccaccagctt catcagcaac ggcgtggtca tcatggaaag
     8581 cgtcaccgtc accagcaaca gcgccatctg gtcgccctgg aataaacgtt gtgaagaagc
     8641 ggtagaaaag aggacaaagg caaactcacc gccctgactc aacacgccag caaactgcat
     8701 ccgctctgag ctacgcacgc catacaatcg cgccagcaga tacagcacga gaattttcac
     8761 cgccaccagc acaaccacgc ttatcactac ccacaacaga tgggtataaa gcaccccgag
     8821 gttgagcgac atgccgacag agataaagaa caaaccgagc agcaagcctt tgaagggatc
     8881 gatagccgtt tccagttcat ggcgatattc actttccgcc agcagcacgc ccgcaataaa
     8941 tgtaccgagc gccatcgaca gccccagcgc atccataaac aatgcggaac ccaacaccag
     9001 cagcagcgtc gcggcggtga acacttcccg cacgccagaa gctgcaataa agcggaatac
     9061 cggacgcagt aaatagcgcc caccaatcag catgccgaca aacgccagca ccttcatgcc
     9121 gatcttcatc cagtcgaaat gttcgtctgc cgaccccgcc aacaacggca ctaacgccag
     9181 tgctgggatc actgccagat cctgaaacag cagaaccgaa aacccgagct ggccggattc
     9241 gctgcgattc atccctttct cacgcatcaa ttgcaacgcc attgcagttg aagacatcgc
     9301 aaggccaatg ccaccgacca ccgccgcctg ccaggcgaaa tccgtcagca tcaataatcc
     9361 cgccagcaac gccgcgctta acagcacctg tgccgcgcct acgccaaaaa tcgaacgccg
     9421 cagttgccaa agtttggagg gattcaactc aaggccgatg ataaacatca ggaataccac
     9481 gccgagttcc gaaaagtgga ggatctcatc gacgtcgcta ataaacccca gcccccacgg
     9541 gccaattgca atccctgcca gcaaatatcc caacacagcg ccaatcccca gccgcgatgc
     9601 caacggcacc gcagccaccg ccgcgaagag aaacagcact cctgcgagta aaaaatcgga
     9661 accttccatc agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg
     9721 gcgtggctcg ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt
     9781 aaccaatgca tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc
     9841 gggtagcgat tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg
     9901 ttacgccagt actttcccgc cagttggttt cctcccggcc cactggcaaa accacgactt
     9961 aatacccggt ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc
    10021 tgaaagacaa tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa
    10081 aaatcgggat agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt
    10141 ttaagcagta cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa
    10201 actttcgctg gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt
    10261 ttgggctacc attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata
    10321 agttaacata gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt
    10381 cgcggcgtgc gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc
    10441 ggcctggtgg gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa
    10501 atcagcgccg acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat
    10561 caggaaacgc cagcattacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa
    10621 tatcgtcaac tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt
    10681 gcgaccattc atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc
    10741 agcctgctgc acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc
    10801 tccggtggct ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg
    10861 ctgctgctcg acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa
    10921 tggttgaaga gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat
    10981 ccgatcgttg ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac
    11041 tacagttcgt ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa
    11101 agccagcagg aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc
    11161 accaaagcga agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc
    11221 cccgcacacg tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat
    11281 ccgttactga agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg
    11341 attaaattga atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt
    11401 aaatcgacat taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt
    11461 ctggcgaaag ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc
    11521 gacgaatcac ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg
    11581 cgtgactacc tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc
    11641 ttctccggtg gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat
    11701 ctgctgctgc tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc
    11761 gaagcattaa tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg
    11821 cgttccacca ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc
    11881 gatctggaag attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac
    11941 gaagcgccaa aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa
    12001 gcggagctgc gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag
    12061 atggagaagc tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg
    12121 tatgaccaga gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa
    12181 tccggcctgg aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg
    12241 ctgctggaag gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta
    12301 gcagcagtgc tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc
    12361 tgccgcgttt gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc
    12421 ccgacggcga ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac
    12481 cgcgtttagt ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc
    12541 tggttgaggc ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca
    12601 gcggtgaacc aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt
    12661 ggtttttacg ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt
    12721 cgctcggcgg taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg
    12781 atgcggcggt gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa
    12841 agggcttttc ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc
    12901 gcaagctggc agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac
    12961 gtcgcatccg tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta
    13021 tcgactatta tcgtcagtgt agcgccatgc cgatgctgaa ccggatcgcc aaaccgacgc
    13081 tgattattca cgccaaagac gatccgttta tggatcatca ggtgatcccg aaaccggaaa
    13141 gtctcccctc gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg
    13201 gcggtacatt acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa
    13261 cgtatctgga ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga
    13321 aaatttaatt gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac
    13381 acttgaacag aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt
    13441 atggtcggag ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc
    13501 tgcaaccgtc ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc
    13561 aaacatccgg tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc
    13621 gcgtttcgta aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt
    13681 tttcaccgtt acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg
    13741 cggcatatca gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc
    13801 attgaatacg gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa
    13861 gccgtaccct ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc
    13921 actgatgtac tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt
    13981 gcgcagcatg tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa
    14041 aaactgatcc gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta
    14101 gtgcgttcaa tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt
    14161 aacttccagc gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg
    14221 tcgctggatg aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct
    14281 tggctgctgg cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct
    14341 ggcggcaaaa tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg
    14401 gaaggaaaga aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac
    14461 aacgggatag ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt
    14521 ttccagcatc aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac
    14581 cgctgcgtct ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag
    14641 gcgtggtgcc tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg
    14701 acgccctttt tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa
    14761 caccatttcc atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg
    14821 accagaggcg gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat
    14881 ttttgctact ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca
    14941 acggaaggcg acctgggtca ggctgaagcg agacaccagg agacacaaag cgaaagctat
    15001 gctaaaacag tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt
    15061 cacattaccg tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc
    15121 aatccagaga cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg
    15181 catggtgctt ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca
    15241 cattcataag tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct
    15301 gtactacatc gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat
    15361 gatcctctcc tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg
    15421 ccaggaacgt agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta
    15481 caaaaaattt cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca
    15541 gatggcgcgt cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt
    15601 gacgggccgc attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca
    15661 cccggacggt atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc
    15721 tcgtgaaacc gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca
    15781 cggtaaaacc atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg
    15841 tagcgcgcca ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac
    15901 tatgcacttc gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc
    15961 gaattacgat tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt
    16021 gatacgcctc ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc
    16081 tgtagcttac tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc
    16141 ggattaacgc tggtacttgg cgtcactgct gagctggggc cattgcacgc aaaattgctt
    16201 cctgcatcgc tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg
    16261 gaaccattgc tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg
    16321 ttctggatct ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg
    16381 gcagattatt gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg
    16441 ctgccaccgc tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag
    16501 caaatgcata tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc
    16561 caggaggcgc tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg
    16621 caaaaactgg tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc
    16681 gccgctcgcc tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt
    16741 acgatggaaa agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg
    16801 gttgggcaat tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa
    16861 ccgctctatg cccgtgactt actggccgat aaacagcggc gaatgccgtt acttccggca
    16921 ctgaaaagtt atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg
    16981 atattaagcg ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc
    17041 ctgatgacgg tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg
    17101 aatcgctccg tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa
    17161 attcccgaag gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata
    17221 ttgcgcaaaa actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg
    17281 caactgttgt ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt
    17341 ggttgtttga ttgctttcgg tggtaccgtc tggctatggc cgcagtggca gagcgggtta
    17401 ttgcgtaaaa acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg
    17461 agcgaggacc cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat
    17521 aacactctgt ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat
    17581 ctggcagata tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc
    17641 atgaccacgc tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta
    17701 cagtcttgtg aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt
    17761 agttctggcg atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca
    17821 ggtacgctgg agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt
    17881 tcgtcgatgg catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg
    17941 gattcgaagg cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat
    18001 attttgcctg atgcgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg
    18061 ttgcatccct tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac
    18121 atccggtccg gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg
    18181 cgcccgacct ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat
    18241 atcgctaaac acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg
    18301 tttcgcctga atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc
    18361 tactgcacag gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc
    18421 agaagcaatt tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt
    18481 cgcagaagtc agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt
    18541 ccgccccatc ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag
    18601 ctcgcgcaag ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat
    18661 cggctcaacc accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc
    18721 gttaaaggga acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg
    18781 ctgcccaccc accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat
    18841 aattttggtt ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc
    18901 ggtttcgtta gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc
    18961 aatcagtttg cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca
    19021 cagagtttcg ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc
    19081 cgtaactgca atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg
    19141 gctgccctga ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc
    19201 atcgaaagtc gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta
    19261 cgcataaata aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa
    19321 tcggaaatca gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac
    19381 tttctggatg gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct
    19441 cgcgggtttc gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca
    19501 ccagcgaatg gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac
    19561 cgctatgtgt aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc
    19621 cgccaaatgc ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc
    19681 cggcatagtg gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag
    19741 gtgagatgac aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt
    19801 tgcgcttaac cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa
    19861 aagaatcgta gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta
    19921 ttttactcag attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg
    19981 agatccccca ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc
    20041 cagctcagtt gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga
    20101 ccacttccca cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc
    20161 tcgacgaact tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc
    20221 agggcattgc cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa
    20281 atatcaactt cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg
    20341 atagtgcgca aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc
    20401 agcgccgtca tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg
    20461 ttacgcatga tatcaaggcc aggataaaga tacggatcgc gcccttcgcc gaatttatcg
    20521 ctcatagtgc ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac
    20581 taaaggcatc tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag
    20641 ctcccagaga cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg
    20701 tgtccatttg ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg
    20761 ggcaagattg cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc
    20821 ggttttgacg gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta
    20881 tcggcaacgt ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga
    20941 ccatggtcaa ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct
    21001 ttgtcagcgg tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct
    21061 tcatttttga ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc
    21121 tgaatcataa agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc
    21181 acataatcga caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg
    21241 atgttaccag ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg
    21301 ggagaaagag cagaaagagc gaaaacagca gccatcgccg ccagggtcga tttgaacatg
    21361 agatttcctt aacaaacaga gcagaaaaaa agcaagtgaa gagattctaa atagccttca
    21421 agaacaaggc catcccttta cctaatttta cgtatctgaa atatatgtaa cctgacgatc
    21481 actttttatt ccgtgatcaa aatcacctct taaaatgcaa tttagcaacc gattgcaata
    21541 aaacatttaa acagatcaca aaatcaccta aaatcgcccg ctcgcggcat atcccgctat
    21601 gccattttat ttttgaacgc aggccaatca tgactaacag caatcgcatc aagctcacat
    21661 ggattagctt tctctcctac gcactgactg gtgcgttggt tattgtcacc gggatggtga
    21721 tgggaaatat cgccgattat ttcaatctgc ctgtttccag tatgagtaac accttcacct
    21781 tcctcaacgc cggcatttta atctctatct tcctcaacgc ctggctgatg gaaatcgtcc
    21841 cgttgaaaac gcagttacgt tttggctttc tcctgatggt gctggcggtt gccggtttga
    21901 tgttcagcca cagcctggcg ctgttctcga cggcgatgtt cattctcggg gtggtcagcg
    21961 gcattactat gtcgattggt acattcctga taacacaaat gtatgaaggg cgtcagcgcg
    22021 gttcccgcct gttatttact gactccttct tcagtatggc tgggatgatt ttcccaatga
    22081 tcgccgcgtt tctactggcg cgcagcattg agtggtactg ggtttatgcc tgcatcgggc
    22141 tggtgtatgt cgctattttt attctgacct tcggctgtga gttcccggca ctgggcaaac
    22201 gtgcaccaaa aacggatgct ccggtagaga aagaaaagtg ggggatcggc gtactgtttc
    22261 tctctgttgc ggcgctgtgc tacatcctcg gtcagttagg ttttatctcc tgggtgcctg
    22321 agtatgccaa aggcctgggc atgagcctga acgacgcggg cacgctggtg agtaacttct
    22381 ggatgtcata catggtcggc atgtgggcgt tcagctttat tcttcgcttc tttgatttgc
    22441 aacgcattct gaccgtactg gctggtctgg ctgcgattct gatgtacgtc tttaacaccg
    22501 gaacaccagc acatatggcg tggtcaattc tcgctctggg cttcttctcc agcgcgatct
    22561 ataccaccat catcaccctg ggttcacagc agaccaaagt accgtcgcca aaactggtta
    22621 actttgtcct gacctgcgga accatcggta ctatgttgac ctttgtggtt accggcccga
    22681 ttgttgaaca tagcggtccg caggcggcac tgcttacagc aaacggtctg tacgctgtcg
    22741 tctttgtgat gtgcttcctg ttaggtttcg tcagccgtca ccgtcagcat aacaccctga
    22801 cctctcatta attgctaatg ccggacggca ctatcgtcgt ccggcctttt cctctcttcc
    22861 cccgctacgt gcatctattt ctataaaccc gttcattttg tccatttttt gcacaaacat
    22921 gaaatgtcag acaattccgt gacttaagaa aatttataca aatcagcaat atacccctta
    22981 aggagtatat aaaagtgaat ttgatttaca tcaataagcg gggttgctga atcgttaagg
    23041 taggcggtaa tagaaaagaa atcgaggcaa aaatgagcaa agtcagactc gcaattatcg
    23101 gtaacggtat ggtcggccat cgctttatcg aagatcttct tgataaatct gatgcggcca
    23161 actttgatat taccgttttc tgtgaagaac cgcgcatcgc ttatgaccgc gtacacctct
    23221 cgtcttactt ctctcaccac accgccgaag agctgtcgct ggtgcgcgaa ggcttctacg
    23281 agaaacacgg catcaaagtt ctggtcggcg aacgcgctat caccatcaac cgtcaggaga
    23341 aggtgattca ctccagcgcc ggacgtaccg ttttttatga caagctgatc atggcaaccg
    23401 gttcctaccc gtggatccca ccaatcaaag gttctgatac tcaggactgc tttgtctatc
    23461 gcaccattga agacctcaac gccattgaat cctgcgcccg tcgcagcaaa cgcggtgccg
    23521 ttgttggtgg cggcctgtta ggtctggaag ccgctggcgc gctgaaaaac ttaggtattg
    23581 aaacccacgt tatcgaattt gcccctatgc tgatggcgga acagcttgat cagatgggtg
    23641 gcgagcagct gcgtcgcaaa atcgaaagta tgggcgtgcg cgttcacacc agcaaaaaca
    23701 cccttgagat tgtgcaggaa ggtgtcgaag cgcgtaaaac catgcgtttt gccgacggca
    23761 gcgaactgga agtcgacttt atcgtcttct ctaccggtat ccgtccgcgc gataagctgg
    23821 caacccagtg tggtctggac gttgctccgc gtggggggat tgtcattaat gattcctgcc
    23881 agacttccga tccggatatc tacgccatcg gtgaatgcgc aagctggaac aaccgtgtat
    23941 ttggtctggt agcaccaggc tacaaaatgg cgcaggtcgc cgttgaccat attctgggta
    24001 gcgaaaacgc ctttgaaggt gctgacctta gcgccaagct gaaactgctg ggcgtagacg
    24061 taggtggtat tggtgatgcg cacggtcgca cgcctggcgc acgtagctat gtttacctcg
    24121 acgaaagtaa agagatctac aaacgcctga ttgtcagcga agacaacaaa accctgctcg
    24181 gtgcggtact ggtgggcgat accagcgact acggcaacct gctgcaactg gtgctgaatg
    24241 ctatcgagct gccggaaaac ccggattctc tgattctgcc agcgcactcg ggtagcggta
    24301 agccgtctat cggtgttgat aaactgccgg acagcgcgca aatctgctcc tgcttcgacg
    24361 tcactaaagg tgatctgatt gctgccatca acaaaggctg ccacacagtt gcggcgctga
    24421 aagctgaaac caaagcgggt actggctgcg gtggctgtat cccgctggtc actcaggtac
    24481 tgaacgcgga actggcgaaa cagggcatcg aagttaacaa caacctgtgc gaacactttg
    24541 cttattcgcg tcaggaactg ttccatttga tccgcgttga aggcattaaa accttcgaag
    24601 aactgctggc gaaacacggc aaaggctacg gttgtgaagt ttgtaaacca accgtcggtt
    24661 cgctgctggc ctcctgctgg aacgaataca ttctgaagcc ggaacatact ccgctgcagg
    24721 attctaacga caacttcctc gctaacatcc agaaagacgg cacctactcg gtgatcccgc
    24781 gttctccggg cggtgaaatc accccggaag ggctgatggc agtgggtcgt atcgcgcgtg
    24841 aatttaatct ctacaccaaa atcaccggct cccagcgcct ggcgatgttt ggcgcacaga
    24901 aagacgatct gccggaaatc tggcgtcagc tgattgaagc tggcttcgaa accggtcatg
    24961 cctatgcgaa agcactgcgt atggcgaaaa cctgcgtggg tagcacctgg tgccgctacg
    25021 gtgttggcga tagcgtcggc ctcggcgtgg aactggaaaa ccgctacaaa ggcatccgta
    25081 cgccgcacaa aatgaagttc ggtgtctccg gctgtacccg tgaatgttca gaagctcagg
    25141 gtaaagacgt gggtattatc gccactgaaa aaggctggaa cctgtatgtt tgcggtaacg
    25201 gcggcatgaa accgcgtcat gcggatctgc tggcagcgga tatcgatcgc gaaacgctga
    25261 tcaaatatct cgaccgcttc atgatgttct acatccgtac cgccgacaaa ctgacgcgta
    25321 ccgcgccgtg gttagaaaac ctcgaaggcg gcatcgatta cctgaaagcg gtgatcattg
    25381 acgacaaact ggggctgaac gctcatctgg aagaagagat ggcgcgcctg cgtgaagcgg
    25441 tagtgtgtga gtggactgaa acggtcaata caccgtccgc gcagactcgc ttcaaacact
    25501 tcatcaacag cgacaagcgt gacccgaacg tgcagatggt gccagagcgc gaacagcacc
    25561 gtccggcaac gccgtatgaa cgtatcccgg taactctggt ggaggacaac gcatgagcca
    25621 gtggaaagac atctgcaaaa tcgatgacat cctgcctgaa accggcgtct gcgcgctgtt
    25681 aggtgacgag caggtagcga ttttccgccc gtatcacagc gaccaggtgt ttgcgatcag
    25741 caacattgat ccgttcttcg agtccagcgt gctatcacgc gggctgattg cggaacacca
    25801 gggcgagctg tgggtcgcca gcccactgaa aaaacagcgt tttcgcttaa gtgatggctt
    25861 gtgcatggaa gacgaacagt tttccgtcaa acattacgaa gcacgagtga aagacggcgt
    25921 ggtgcagctg cgcggttaat gttttaacgg gaggcgcaat gcctcccctt tttgcatggt
    25981 cctgtaatga ccttcggtat attgcaggac attttttaaa ctttttgttt tattttttgt
    26041 ttttattttt taaaggataa tcaaatgttt acagacacta ttaataagtg tgcggctaat
    26101 gctgcgcgca ttgcacgcct gtcggcaaat aacccgctcg gcttttgggt cagctccgcc
    26161 atggcgggcg catatgtggg tcttgggatc atcctgattt tcacgctcgg taatttgctc
    26221 gacccgtccg tacgtccgct ggtgatgggc gcgacctttg gtatcgcctt aacgctggtg
    26281 attatcgccg gttctgaact gttcaccgga cacaccatgt tcctcacctt tggggtaaaa
    26341 gcgggcagca tcagccacgg gcaaatgtgg gcaatcctgc cgcaaacctg gctgggtaac
    26401 ctggtcggtt ccgtcttcgt tgccatgctc tatagctggg gcggcggtag cctgctgccg
    26461 gtagatacca gcatcgttca ctccgtcgcg ctggctaaaa ccactgcacc ggcaatggta
    26521 ctgttcttca aaggtgcatt gtgtaactgg ctggtttgcc tggcaatctg gatggcgctg
    26581 cgcactgaag gggcggcgaa atttatcgct atctggtggt gtctgctggc atttatcgcg
    26641 tccggctacg agcactctat cgctaacatg acgctgttcg cgctctcctg gttcggcaac
    26701 cacagcgaag cctacacgct ggcgggtatt ggtcataacc tgctgtgggt gacgctgggt
    26761 aatactttat caggtgccgt attcatggga ttgggttatt ggtatgctac gccgaaagcg
    26821 aatcgtccgg ttgcggacaa atttaatcaa actgaaacgg ctgccggtta attactaagg
    26881 ggtttttacg tggatcattt gcctatattt tgccaattac gcgatcgcga ctgtctgatt
    26941 gtcggcggtg gtgatgtcgc ggaacgcaaa gcaaggttgc tgttagacgc aggcgctcgc
    27001 ttaacggtga atgcattagc gtttattcca cagttcaccg catgggcaga tgcaggcatg
    27061 ttaaccctcg tcgaagggcc atttgatgaa agccttctcg acacctgctg gctggcgatt
    27121 gcagcgacgg atgatgacac gcttaaccag cgcgtcagcg aagccgctga agctcgtcgc
    27181 atcttctgta acgtagtcga tgcgccgaaa gccgccagct ttattatgcc gtcgattatt
    27241 gaccgctcac cgctaatggt agcggtctcc tctggcggca cctctccggt tctggcgcgc
    27301 ctgttgcgcg aaaaactcga atcactgctg ccgttgcatc tgggccaggt ggcgaaatat
    27361 gccgggcaat tacgcgggcg agtgaaacaa cagttcgcca cgatgagtga gcgtcgccgt
    27421 ttctgggaga aattgttcgt taacgaccgc ctggcgcagt cgctggcaaa caacgatcag
    27481 aaagccatta ctgaaacgac cgaacagtta atcaacgaac cgctcgacca tcgcggtgaa
    27541 gtggtgctgg ttggtgcagg tccgggcgat gccgggctgc tgacgctgaa aggactgcaa
    27601 caaattcagc aggcagatgt ggtggtctac gaccgtctgg tttctgacga tattatgaat
    27661 ctggtacgcc gcgatgctga tcgcgttttc gtcggcaaac gcgcgggata ccactgtgta
    27721 ccgcaggaag agattaacca gatcctgctg caggaagcgc aaaaaggcaa acgcgtggtg
    27781 cggctgaaag gtggcgatcc gtttattttt ggccgtggtg gcgaagagct ggaaacactg
    27841 tgcaacgcgg gtattccgtt ctcggtggtt ccgggtatta ccgcagcttc tggttgctct
    27901 gcctattcgg gtattccact cacgcatcgc gattatgccc agagcgtacg cttaattacc
    27961 ggacacttaa aaaccggtgg cgagctggac tgggaaaacc tggcggcaga aaaacagacg
    28021 ctggtgttct atatggggtt gaatcaggcc gcgactattc agcaaaagct gattgaacac
    28081 ggtatgcctg gcgaaatgcc ggtggcaatt gtcgaaaacg gaacggcagt cacgcagcgc
    28141 gtgattgacg gtacgctcac gcaactcggt gaacttgctc agcaaatgaa cagtccatcg
    28201 ctaattatta ttggtcgggt tgttggcctg cgcgataaac tgaactggtt ctccaatcat
    28261 taatttaacc cgggccagag aattctggcc ttcttaatga ctctttttat atattcagca
    28321 aataaaacat aaccccttaa taaatattat ctgc
//
LOCUS       DBKEKI010000047        28325 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.047, whole genome shotgun sequence.
ACCESSION   DBKEKI010000047 DBKEKI010000000
VERSION     DBKEKI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.047"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="ACUQPX_001955"
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="ACUQPX_001955"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HHV9056678.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(998..2020)
                     /gene="ytfT"
                     /locus_tag="ACUQPX_001956"
     CDS             complement(998..2020)
                     /gene="ytfT"
                     /locus_tag="ACUQPX_001956"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HHV9056679.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /gene="ytfR"
                     /locus_tag="ACUQPX_001957"
     CDS             complement(2034..3536)
                     /gene="ytfR"
                     /locus_tag="ACUQPX_001957"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HHV9056680.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQFGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="ACUQPX_001958"
     CDS             complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="ACUQPX_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HHV9056681.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            4942..5472
                     /gene="ppa"
                     /locus_tag="ACUQPX_001959"
     CDS             4942..5472
                     /gene="ppa"
                     /locus_tag="ACUQPX_001959"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HHV9056682.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5852..6193)
                     /locus_tag="ACUQPX_001960"
     CDS             complement(5852..6193)
                     /locus_tag="ACUQPX_001960"
                     /EC_number="4.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061929 -
                     gamma-glutamylaminecyclotransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HHV9056683.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6196..9975)
                     /gene="tamB"
                     /locus_tag="ACUQPX_001961"
     CDS             complement(6196..9975)
                     /gene="tamB"
                     /locus_tag="ACUQPX_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0089705 - protein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HHV9056684.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVLVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9972..11705)
                     /gene="tamA"
                     /locus_tag="ACUQPX_001962"
     CDS             complement(9972..11705)
                     /gene="tamA"
                     /locus_tag="ACUQPX_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0089705 - protein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HHV9056685.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11911..12540
                     /gene="msrA"
                     /locus_tag="ACUQPX_001963"
     CDS             11911..12540
                     /gene="msrA"
                     /locus_tag="ACUQPX_001963"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HHV9056686.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGVCLPPEA"
     gene            12863..14206
                     /locus_tag="ACUQPX_001964"
     CDS             12863..14206
                     /locus_tag="ACUQPX_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HHV9056687.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14268..14474)
                     /locus_tag="ACUQPX_001965"
     CDS             complement(14268..14474)
                     /locus_tag="ACUQPX_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HHV9056688.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14799..15353
                     /locus_tag="ACUQPX_001966"
     CDS             14799..15353
                     /locus_tag="ACUQPX_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HHV9056689.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15416..16354)
                     /gene="ytfI"
                     /locus_tag="ACUQPX_001967"
     CDS             complement(15416..16354)
                     /gene="ytfI"
                     /locus_tag="ACUQPX_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HHV9056690.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16566..17306)
                     /gene="cysQ"
                     /locus_tag="ACUQPX_001968"
     CDS             complement(16566..17306)
                     /gene="cysQ"
                     /locus_tag="ACUQPX_001968"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HHV9056691.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17496..19434
                     /gene="cpdB"
                     /locus_tag="ACUQPX_001969"
                     /pseudo
     CDS             17496..19434
                     /gene="cpdB"
                     /locus_tag="ACUQPX_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
     gene            complement(19560..19940)
                     /locus_tag="ACUQPX_001970"
     CDS             complement(19560..19940)
                     /locus_tag="ACUQPX_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HHV9056692.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGGLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20029..20889
                     /gene="qorB"
                     /locus_tag="ACUQPX_001971"
     CDS             20029..20889
                     /gene="qorB"
                     /locus_tag="ACUQPX_001971"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HHV9056693.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEATLTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            20998..21963
                     /locus_tag="ACUQPX_001972"
     CDS             20998..21963
                     /locus_tag="ACUQPX_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HHV9056694.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22071..22733
                     /gene="ytfE"
                     /locus_tag="ACUQPX_001973"
     CDS             22071..22733
                     /gene="ytfE"
                     /locus_tag="ACUQPX_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HHV9056695.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22778..24190)
                     /gene="cycA"
                     /locus_tag="ACUQPX_001974"
                     /gene_synonym="dagA"
     CDS             complement(22778..24190)
                     /gene="cycA"
                     /locus_tag="ACUQPX_001974"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HHV9056696.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24498..25118)
                     /gene="fklB"
                     /locus_tag="ACUQPX_001975"
     CDS             complement(24498..25118)
                     /gene="fklB"
                     /locus_tag="ACUQPX_001975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HHV9056697.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25337..25882
                     /locus_tag="ACUQPX_001976"
     CDS             25337..25882
                     /locus_tag="ACUQPX_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HHV9056698.1"
                     /translation="MPGRFELKPTLEKVWHALDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25866..26513)
                     /locus_tag="ACUQPX_001977"
     CDS             complement(25866..26513)
                     /locus_tag="ACUQPX_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHV9056699.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKVIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26725..27516
                     /locus_tag="ACUQPX_001978"
     CDS             26725..27516
                     /locus_tag="ACUQPX_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HHV9056700.1"
                     /translation="MKSTRPVAVITGAARGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27526..>28325
                     /locus_tag="ACUQPX_001979"
     CDS             27526..>28325
                     /locus_tag="ACUQPX_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HHV9056701.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGLPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6943 a         7544 c         6994 g         6844 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccacc aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacgat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acggcttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt ggctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca aactggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccacttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agcctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     4141 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
     4201 ttcttttacc agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt
     4261 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat
     4321 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     4381 aaagatcgca tccacccctt gtgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     4441 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     4501 cacattggtt tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     4561 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
     4621 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
     4681 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
     4741 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
     4801 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
     4861 cgaaaacaag caaagacatt cggcgcgagt tggctataat actcggcact tgtttgccac
     4921 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
     4981 gaagacatct acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc
     5041 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
     5101 tgcaactacg gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg
     5161 gtcccgactc cgtacccgct gcagcctggt tctgtgatcc gttgccgtcc ggttggcgtt
     5221 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag
     5281 ctgagcaaag aatacgatca cattaaagac gtaaacgatc tgcctgaact gctgaaagcg
     5341 caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt
     5401 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca
     5461 aagaataaat aagttcttct agcgcaataa ccctgaacgc cgggcttcgg ttagtaaggg
     5521 tttttttatg cccgcgataa ataaactctc tattccacca tcattattct cagcggttgc
     5581 aaggcttgaa cggtaagaac aagcaaaccc gaccaccatt ttgctgttca tagccacttg
     5641 ctggaagtta gccgacctca ctcatactca ccgttcaaag cgtgcccccg ttttacccct
     5701 gcacatttgt gaacgccagc agagtttttt atgcgattgc agatatggaa agcggctcgg
     5761 agagaaaagc aaaagatgga aaagttacaa aaaaaaatcg cgagatacat cgcataaaac
     5821 aacgccaccc gaaggtggcg tgcttttatt attacttatc cctgtctaac cagtcgccgc
     5881 tttcaattag ctttaatcca tcgacgggtc gttgataaac gtacatccat gcactcccat
     5941 acggcgtctg aatcaactgg cgcgcgtatt caccgcccct ggtgcgcaag gcatcaagtt
     6001 cggccagcgt ggcgttgtca atacgataaa cttcaccgtg taccgttccg ttccccggaa
     6061 ctgcgcctgg atagtggccc aggctataca actggtagtt atcgatactg aaatcgccca
     6121 gtaactgggc attggtcatc cagtgactgt tgccttgttt gtggcgtaaa ctgccgtaga
     6181 caaatattcg cattgctaaa actcgaactg atagagcaaa tccagtgcct ggtctacacc
     6241 agacacggct tccagatata gcttaggcat caggcgataa cgtaacgtga gtgttgctat
     6301 agagtcaaat atacccacgc cgtatttcac ttgcagacct ggcaatacat agccgctgac
     6361 cactacctgg gaggagtcgc ctactcccag ggtgtcgagc gctaaattgc ttacgccaaa
     6421 cgtctcgccg attttaccca caatctggcc actttgcgca acccccagac caatcagcat
     6481 cgaggtcatt gccgcactgt cgctctgatc gctctccagc ccttgtccac gtagcaaata
     6541 agacaatgca gcttgttgcg acatcgccgg gtcagagaag atctccgctt tcggttcgtc
     6601 cgccagacca gtgacgcgaa ctccggcgat tacatcgtct tctgtagcat ccgggttacg
     6661 aatagcttca atattaagat acggttgatc cggcggacca gagaacagta actcaccctt
     6721 acgcacaatc agatcctgac cataggcatg gaagcgccct tcagggatgt tgatctgccc
     6781 gttcaggccc agcccttgtt tgtcctgtac gacgttaaga tcgcccgtca gccgcgcttt
     6841 caggccaaag gcgtcaatgc gcacattgtt gccaacgtgg acaatcaggt tactgttaat
     6901 cggaatcgac gccgttttcg gctcttccgg ttgcaggtta tcgttaagca tcaccacatc
     6961 gctggagacg cctactgcgc tttccggcag atcgtgcacc acgatgcgcg cccacgggac
     7021 atccacgcga ccatcgaggg taaacaggtt tggtgtagcc tcgaatacaa catctggcga
     7081 tacatccatt cgtaccatcg gcggcacggt gatccgcact ttactgcctt tcgccgttac
     7141 tcgtgcccgc cagttttcaa tttggctcca gtcggcatca ccgttcaggt agatttcacc
     7201 ctgctgggtc cgtactgtac cggcaagcgt cgagcgcata ccgttaaagt tgaccgcaag
     7261 ctggctcggc tgcatatcaa acggcataaa gttgccgtcg atatccacac ccgtaacctg
     7321 aagctgacca aacaactgcg ggctttgcac atcaccaccc agacgcaagt tggcactcac
     7381 catccccgct gctttttccc cacgggtaaa gatggggttt atcatcgcaa ggttgaagtt
     7441 acggatattg acgttgccac caagattacg gcggccttgc ggatcggtca cctgcacctg
     7501 tccatcaaac tggccgttat tggtcaggcg gatggtccag cccaattcgg cacggttgtt
     7561 acgcaattcc gccgtcagat tcagtgtctg aaacgccacc ggcagcgccg catcgttgac
     7621 ggtttgcgtt acctgcacgt tacgccccga aagggtgata ctgccctgcg gcagcccctc
     7681 tttcgtggtg tcccaggcaa catccgcttt acccgtgaag ataccgctgg cctgagtggt
     7741 ttctggcata aacggtttca gcatggcgag gtcgaagcgg ttgagattca ccaccgcacg
     7801 cccttcggcc cccgcatcga tagtttgcgg cacgcacagt tccgcattcg ggttaagcca
     7861 gcagtgtggc ccgatgctga ttttttgctc cttattgcgg taatccagcg caatatcgcg
     7921 ggtcagcgac cacgggccga ccggcgtctg gaagcgggta ttgctaagag ttcccttcca
     7981 gcgttcttct ttgcgatcaa aacttcctgc cagattaagc tgcccggaga caggctcgcc
     8041 ctgaatccgc aactgtagct cgtgctgctt ttcgctgcct ttggcattca gggtgacgag
     8101 gttgatattt acatccggct gcgaaatttg ctcaacgcgt acgtcgagtt tcccggcgat
     8161 ctgatcggtg gatttaatgt cgccttccac gcgaacctgc gccacggaaa gttcctgcca
     8221 gcgcaggccg cgcgcggtga tatctgccag tagttgtggc gcttccaccg tgccgcgtac
     8281 tttcaccagc cctttcgctg taccgccaag ccccggcagc gcgttatcca gccccggcgc
     8341 gttgatggtg gcatcaagat tgagatcttt tacccccagc tcgcctttca cttcggcact
     8401 gtttggcccg agttccagat gaagcccagg gatcatccac tgcatataac tgttgccttt
     8461 cagcgtgccg tcaacgttca ctttgttctg tttgacgtta ccggtcagct tcagttctgg
     8521 cacctccatc tgccaggtgc cgccgtacag gctaccgcgg gttttaatca agccattgag
     8581 tttcgacggc cactccggga actctttggc ggtgttaatg ccattaagcg ttagctcacc
     8641 gcgccaacta atggcctgct gccagtcgag caacgccttg agttcagttt tcccttccag
     8701 cgccgcgacg gtgagtttgt cgagattgac ctgctgttca ttacctttgg catcaagggt
     8761 aatggtggct ggcgggatct cctgtccctt cactgccgta cgcatagaga gcgtgtaatc
     8821 ggtcattttg ccggtaagtt tcagtttcag atcatccgcc tgatactgct tctcaccagt
     8881 gaacggccag taaagctgtt tgctgttcac ttccacgttg agtggcaatc cggcttccgc
     8941 cagtcgcgtc tgggcgcgta aatccatatc caccggaccg gaaaggttaa cgcctatctc
     9001 cagctgttcg cgcagcgcgc cgcccacttt cagcttcacc ttttcacctt tcaacggctc
     9061 cacgttcagt gtgctgttga gggtgatatc caccggccag ttgtctgaca gctgcgccgt
     9121 gccgctggcg ttgacgatcc cctggttgga atcgatatcc agggcgtcca gtttagtatt
     9181 gccgtcaatg ctgctcactt tcagcagcat ggtgcgcacg gtgatgtccg tgtcgcccgt
     9241 cacgcgcagc tgctcgccct taaactcttc aatgttcagg ttaagcggca aatgcacgtc
     9301 ggtcatttcc ggcaataccg ggcgagaaaa gagatctttc agcgtttcgc cgagcggctt
     9361 ttcctccggc tgcggatttt caattttcgg ttcgaccact tcttcctgcg ccacttccac
     9421 cactttcggc agagcaatca gcaggccttt cagcgacgtc ggtttcaggg tcagggtttt
     9481 ctcctgccag ttcaggccgg aggtgaagtc catcaccaac accgtggtgt catcaatctt
     9541 gatgttgacg ttgtccagcg ctacccgtgt cagggtgatg ggatacggcg tggagagatc
     9601 cagcggaccg ctatcttcct cttcttcaac ctgttcagaa ggaggcattt ttttactgtc
     9661 gatgttgacc tgaatgtctt tcagcgccag gtcattaata caaacgctac tgttccacag
     9721 gcactcaaga ccgaccgcca gatgcagatt acccgctttt accgccacgc ctggctgctc
     9781 ataacgaacg tcagacaagg tgagatcgcg ccagccgccg gtgaccttgc caatatccag
     9841 tcctggcacc cagcgatccg ccgctttaaa tacaagatgc aggccgctgg tggtgcccac
     9901 cagaaacgcc accgatccca gcaacagtaa gataacgata accacgccga ggctgatttt
     9961 tttccataaa ctcataattc tggccccaga ccgatgtaaa actgtaaccc gtgttcgtct
    10021 ttatccgcga ccggtacggc aaaatcgagt ttgattggcc cgaccggcga ttcccagcgt
    10081 acgccgaccc cggtaccggt tttaaagtcg ctgcggcgaa tatcgcttac cgcttcgcca
    10141 ctatcgacaa acaccgcgcc ccaccatttt ccggtcacgt tgtactggta ttccagcgat
    10201 ccggttatca acttcgaggc ccctttcagg tcaccgttgg cgtatttcgg ggcgatagat
    10261 ttgtatttgt agccgcgaat actgcgatcg cccccggcga agaaacgcag atccggcggt
    10321 actttgtcga aatcaccggt ttcaatccag cccagcgtgc cgcgtgtcac aaagcgatgg
    10381 cgatcgtaca gtgtgcggat ccagacgttc tgcgcctgga aaacggagaa atcgacatct
    10441 gaaccccagg ccgtgttgga gtagtcgata gagtagcgtt gcgagtcgcc ccaggttggc
    10501 atcaggccac cacgagaacg cgtgcggcta atcatcaccc caggataaaa cagcatcgtg
    10561 gtgttggtaa tttcaccctg ggtaaagtgg tcgaggctcc agcgcaggtt aatggcacgc
    10621 tgccagccgc tggagagatc ccagtagcga gaagccacca gcgtagtgga gtcagattcg
    10681 gtatcgttca ggtcagtgcg cttaaaaccg ccctgcacca aataatattg ttccagtgga
    10741 ttcttcagca gcggcatttt atagctgaag tcgagggtct gttccggcgc ggaaatactg
    10801 gtactggtgg tcaaactgtg accgtatgag ttcatccacg gctttttcca cgtcgctttc
    10861 acgcgcggtc ccacgtccgt agagtaaccg accccggttt cgatggtgtt ttctgttcgc
    10921 ggcgaaacca cgcccgtcaa tggtaatact ttcgtttcgc gcgctttatc aaattgtgga
    10981 gccaccacca ccgagttaaa ccagccggta gcagaaagtc ggcggttcag ttccgccaga
    11041 tctttcgatt catactcatc gccctcttta aacggcacca gattttgcag gtattcatcg
    11101 cggatttgtg atccttcaaa ggtcacatgc ccaaagcggt aacgttcgcc actgttataa
    11161 tcaatatccc agaaggcttt atgcaggccg agcgcaatgc ccagctgcgc tttggtaaat
    11221 tcgctatcga aataaccttt acgcaacgca atactggtta aggacttttt gaaattttca
    11281 taatcgccct ggttcagtac cgtaccaata gccgggcgag tatcgagcaa tttcaaatag
    11341 tctttatcgg tccgcgcgcc gccgcgcaat accacatcgg tgccgccaat taacaccggc
    11401 acacctggcg tgactttggc gatcaatacc tgccgccctt tctttggcgg tggacggaga
    11461 tcaaattcaa tggtcggctg gtaataaccc agcgctttca gaccttcacg gatggcatca
    11521 tcgacgcgtg cgcgaaagcg acggtctggc gtcacttcat cactttcaat cgtagaaagc
    11581 tgcgcacgaa cgttcttttc cagctgtccc gataacccct cgacctgtag acggacgttc
    11641 gcggcgacgg cagatccgct taagcagagt aagcttacac agcataactg tcggatatag
    11701 cgcacatttt ctcctgaata tccttttttc ctgcccctgg aacgccgtta aacggcttaa
    11761 caaaaatcca gtaatatgga ttaaaaagca gactaaaccc caaatatttc ttatgtttta
    11821 ctttagacct attcacggtg gttattgtgt gcaaatacgc ctcttgttac aaccttaacc
    11881 ccaatgaccg attttcggga gagcgacacc atgagtttat ttgataaaaa gcatctggtt
    11941 tcccccgccg atgccctgcc tggacgtaac accccgatgc ccgtagccac gctgcatgcg
    12001 gtcaacggtc actcaatgac caatgtacct gacggaatgg agattgccat ttttgcgatg
    12061 ggttgtttct ggggtgtgga gcgtctgttc tggcagttac ccggcgttta cagcaccgcc
    12121 gcaggctata ccggcggcta tacgccaaat ccgacttatc gggaagtgtg ctccggtgat
    12181 acgggtcatg ccgaagcggt acgcattgtt tacgatcctt ccgtcatcag ctatgagcag
    12241 ttgctacagg tattttggga gaatcacgat cccgcccagg gaatgcgtca gggcaatgac
    12301 cacggcacgc agtatcgttc agcgatttat ccgctgaccc cagaacagga tgccgcagct
    12361 cgcgccagtc tggaacgttt tcaggcggcg atgcttgccg ccgatgacga tcgtcacatc
    12421 accacggaaa tcgctaacgc cacaccgttt tattatgccg aagatgacca ccagcaatat
    12481 ctgcataaaa acccatatgg ttactgtgga attggcgtct gtctgccgcc ggaagcatag
    12541 cgttacgggt acaaatgtag tttgttgata aagtgctctt tgtctatgcc tgatgcggct
    12601 taaacgactt accaggccta caaaatcgtc caaattcaat atattgcaag gactgcgtag
    12661 gcctgatcgg catagcgcat caggcagtat tgcatttatc agcagtctga attttaaccc
    12721 tctggcgact ttacagtacc ttacgctata ctagccactg aaaatgccgg ttcactttct
    12781 tcgaatcggc tttcaatgtg tatttcacac aaattaatca actttccctt ccgaggatct
    12841 ggcctgaaag gtcggataag atatgttaaa cagtatttta gtcatactct gcttgatcgc
    12901 tgtaagtgcg ttcttctcga tgtccgagat ctcgcttgcc gcctcacgca aaatcaaact
    12961 taaactgctg gctgatgaag gcaatataaa tgcccaacgc gttctgaata tgcaggaaaa
    13021 tcccggcatg ttctttaccg tggtccaaat cggtctgaac gcagtggcga ttctcggcgg
    13081 tatcgtcggt gatgcggcat tttctccagc ttttcacagc ctgttctccc gctatatgtc
    13141 ggcagagctc tctgagcaac tgagctttat tctctctttc tcgttagtga ctggcatgtt
    13201 tatcctgttt gcggatttaa ccccgaaacg catcggtatg attgcgccag aagcggtggc
    13261 tttgcgtatc atcaacccga tgcgcttctg cctgtacgtt tgcaccccgc tggtgtggtt
    13321 cttcaacggc ctggcgaaca tgatcttccg tattttcaaa ctgccaatgg tacgtaaaga
    13381 tgacatcact tctgatgaca tctacgcggt agtggaagcc ggtgcgctgg cgggcgtgtt
    13441 acgtaaacag gaacacgagc tgattgaaaa cgtctttgag ctggaatccc gtaccgttcc
    13501 gtcttcaatg acaccgcgtg aaaacgtgat ttggtttgat ctccacgaag atgagcaaag
    13561 cctgaagaat aaggtggcgg aacatccgca ctctaagttc ctcgtctgta atgaagatat
    13621 tgaccacatc atcggttatg tcgattctaa agacctgctg aaccgcgtgc tggctaacca
    13681 aagcctggca ctgaacagcg gcgtacaaat tcgcaacacg ctgattgtgc cggatacgtt
    13741 aaccctttca gaggcgttgg aaagttttaa aaccgcaggt gaagacttcg cggtgatcat
    13801 gaacgagtac gcgctggtgg tggggatcat caccctcaac gacgtgatga ccacgctgat
    13861 gggcgatctg gtcggtcagg ggctggaaga gcagattgtc gcccgtgatg agaactcatg
    13921 gctgattgac ggcggcaccc caattgacga cgtcatgcgc gtgctggata ttgacgagtt
    13981 cccgcagtcg ggcaactacg aaaccatcgg cggctttatg atgtttatgc tgcgtaagat
    14041 cccgaaacgc accgattcgg tgaaattcgc cggctacaaa tttgaagtgg tggatatcga
    14101 taactaccgc atcgaccagc tgctggtgac ccggatcgac agcaaggcca ccgccctttc
    14161 gccaaaactg cctgacgcta aagataaaga agaaagcgtc gcgtaacccc cagaaacatc
    14221 aacggctcct gaatcaggag ccgtcttatt actgcatagc actttggtta agccatctct
    14281 gtttgcagac gcataacctg acggttaact tcggacatca ctgacaaatg cagcttatcc
    14341 ttcacttttg ggataagaat cttaccctta tcaaattcaa aagcgccaac gtccttgatg
    14401 tataaccgtc cacggaacag gatctttacg tacttcgcca cctgaagtgg gttgtaccgt
    14461 tggaaaattt tcattgttat ctcctgctga gtattacgcc tgtgcggtgc cacaatcggc
    14521 ccaactatta tgaggcgcaa attttaatgc ttagtgacta tagactatcc gggcaatgtt
    14581 tccaccgcgt ataacttttt ttacctaaag gttacaatta ttcagaatta tctttttacc
    14641 gaagcgcggt cttcagtata agcattcatt tttcatatga tttgtgcgct tgaccgcaaa
    14701 ctggcatcac acttgcggga aattcgataa atagcacata tgattaaaac tcagacccaa
    14761 gtggtcggat cacctgcata tcataagaag gaaacaccat gaccctacgc aagattctgg
    14821 cactcacctg cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa atcggtcagc
    14881 gagtgccgcc gattggcatt accgatcggg gcgagttggt gcttgataaa gatcagttta
    14941 gctacaaaac ctggaacagc gcgcagttag tgggaaaagt gcgagtactg caacatattg
    15001 ctggtcgcac ctctgcaaaa gagaaaaacg cgacgctgat tgaagcgatt aaatcagcga
    15061 agttaccgca cgatcgttac cagaccacca ccattgttaa caccgacgac gcgattccgg
    15121 gttccggcat gtttgtgcgc agcagtctgg agagtaataa aaagctttat ccctggtcgc
    15181 agtttattgt cgatagcaat ggtgtcgcgc gcggtgcctg gcagctggat gaagaaagtt
    15241 ccgctgtagt ggtgctggat aaagacggtc gcgtgcaatg ggccaaagac ggggcgctca
    15301 caccggaaga ggtgcagcaa gtgatggacc tgctgcaaaa gttgctcaaa taatggcaat
    15361 aacataaatt ctggaaaaat aattttcccc tatcgttatc tatgggatag gggaattaag
    15421 tctctgagtt cttacaccga actaatactg gtaacgctag ttgctctttt ttaaacgcct
    15481 caatggtcga tttatatttc tcgcaaatct tttctttggt aatattacta tctgcggcca
    15541 gattcgcact tactatcgca tccacttgtt cggctaattc gacttgttgg caacctgcat
    15601 taaccagaaa attataaata agagagtaat cttcatcctc caaaacaaca gaggaaccat
    15661 tacgaaaacg ttcatcccct ccatagattt taagcgcaaa gcccaaagat tctatgacta
    15721 tggcaaaatt atcatcaatt attttttcgt aaatatacca tccatcgcat tctttaattc
    15781 caccgcctgg tatttggagt tcaaatttac cccgttttaa tttgcttact acatcctcca
    15841 ataaggcata accgaaagat acgtcaccaa acgcctggcg aatataacct ttaatccttt
    15901 ttggtatgtc agactgtttt ttctgcaaaa tatcacaggc atacaatctt ctgttttgtt
    15961 ctaacggaaa ctcgcggttt ttgaaatcca tgaatgagtt atactcattt attgttatgg
    16021 gaatttcttc gtattcaaac ccactgcctc gcaatgtcat tgtatattca gcaccgttat
    16081 ctttaaggct gaaactgata agaaaattat ccatggcatt aacagtttca ttttttgatg
    16141 aaagcacttg gtaactattt ttatcaatct gtgttgcgca acgaaaggac tcccgcaaag
    16201 ttaacagact atcataattc gaaacccaat cttttttctt aaatgcatgc tgaatattat
    16261 taaaaaaatt gtttgaatgt gtttttgtat gcgatgactt tacaaataac tccaccgcac
    16321 caataaaatt attgtgtctg atcctgggta acatatggct accttatttt atttggttat
    16381 tttatgtcgc cattaaatat ctcattatag aagtaaacta tcaaaaaacg ctcattatta
    16441 aaatgtaact tattagcaat tgaatttttg cactttaaat acaatctctt tttatttata
    16501 actttttagg ttgggataaa tcaggatggc agaatcagga aatacactgt ttctgccatc
    16561 tgaatttagt aaatggacac tctgaacccc ggattcagga acgactcgcg cggagtgtaa
    16621 tccagcggtt taccctgcca gtcgtgaacg tgcgctccgg cagctgcagc cacagcatgt
    16681 ccagcggcgg tgtcccaaat attcgttggt ccgaagcgcg ggtacagctg cgcctgtcct
    16741 tccgccacca ggcagaattt cagcgaagag ccgatggacg tggtctgatg ttcgccaagc
    16801 tgttgcagat actctttcag ctccgcatct gcatgggaac ggctgataac caccagcggc
    16861 gggcgcgcat cgcggacctg aatcagcttg cgcacaccgc actcttcttt ccaggccttg
    16921 ccttctgccg cgctgtacat tacgttcatt accggcgcat acaccacacc taaaatcggt
    16981 ttgccatggt caatgagcgc aatgttaacg gtgaattcgc cattacgttt aataaactct
    17041 ttagtaccat ccagcgggtc taccagccag taacgctgcc agtgctgacg gacttcccaa
    17101 ccgggaggat cttcttcaga aaggaccgga atatccggtg ccagcgtacg taaaccgtcc
    17161 atgataacgg tgtgagcggc aatatccgct gccgttaccg gagaattgtc cgctttgctg
    17221 acgacgtcca tcggtttcgt cccgtcgtag acctgcataa tggcatcgcc tgcattccgt
    17281 gcaagctggc atacttgatc taacatttct ccacctcgtc tctgtgagcg gtgttaactt
    17341 attgttttac ttatacccta tcgttaatga atgcgccaac tgtgatagtg tcatcatttt
    17401 caaagcgtaa aattgtggca ttcttcactg ttctataagt aaggcgttta ttctcccttt
    17461 tctttcgtat tcccgatgat aaaaggatgt ccctgatgat taagtttagc gcaacgctcc
    17521 tggccacgct gattgccgcc agtgtgaatg cagcgacggt cgatctgcgt atcatggaaa
    17581 ccactgatct gcatagcaac atgatggatt tcgattatta caaagacacc gccacggaaa
    17641 aattcggact ggtacgtacg gcaagcctga ttaacgatgc ccgcaatgaa gtgaaaaaca
    17701 gcgtactggt cgataacggc gatttgattc aggggagtcc gctggccgat tacatgtcgg
    17761 cgaaaggatt aaaagcaggt gatgttcatc cggtttataa ggcgctgaat acgctggatt
    17821 atacggtcgg tacactcggc aatcatgaat ttaactacgg tctggattac ctgaaaaatg
    17881 cgctggcggg agcgaaattc ccttatgtaa atgccaacgt cattgacgcc agaaccaaac
    17941 agccaatgtt tacaccgtat ttaattaaag ataccgaagt ggtcgataaa gacggaaaaa
    18001 aacagacgct gaagattggc tatattggcg tcgtgccgcc gcaaatcatg ggctgggata
    18061 aagctaattt atccggaaaa gtgacggtga atgatattac cgaaaccgtg cgcaaatacg
    18121 tgcctgaaat gcgcgagaaa ggtgccgatg tcgttgtcgt tctggcgcat tccgggctgt
    18181 ctgccgaata aagtaatggc ggaaaactca gtttattacc tcagtgaaat tccgggcgtt
    18241 aacgccatta tgtttggcca tgctcacgcc gttttcccgg gtaaagattt tgctgatatc
    18301 gaaggggctg atatcaccaa aggcacgctg aatggtgttc cggcggtaat gccgggcatg
    18361 tggggcgatc atcttggggt ggtcgactta caactcagta atgacagcgg taaatggcag
    18421 gtgacgcagg cgaaagcgga agcacggccg atttacgaca tcgccaataa aaaatccctc
    18481 gcggcggaag acagcaagct ggtagaaaca ctcaaagccg atcacgatgc cacacgccag
    18541 ttcgtcagca agccaatcgg taaatctgcc gacaatatgt atagctatct ggcgctggtg
    18601 caggacgatc cgaccgtgca agtcgtgaac aacgcgcaaa aagcgtatgt cgaacattac
    18661 attcagggcg atccggatct ggcaaaactg ccggtgcttt cagctgccgc accgtttaaa
    18721 gttggtggtc gcaaaaatga cccggcaagc tatgtggagg tggaaaaagg ccagttgacc
    18781 ttccgtaatg ccgccgatct ttatctctac cccaatacgc tgattgtagt gaaagccagc
    18841 ggtaaagagg tgaaagagtg gctggagtgc tccgccggac agtttaacca gattgctcct
    18901 aacagcacga aaccacagtc actcatcaac tgggatggtt tccgcactta taactttgat
    18961 gttattgatg gtgtgaatta tcagattgat gttacccaac ccgcccgcta tgacggcgag
    19021 tgccagatga ttaatgccaa tgcagagcgg attaagaacc tgacctttaa tggcaagccg
    19081 attgatccga acgctatgtt cctggttgcc accaataact atcgcgctta cggcggcaaa
    19141 tttgccggta cgggcgacag ccatatcgct tttgcttcac cggatgagaa ccgctcggtg
    19201 ctggcagcgt ggattgctga tgagtcgaaa cgtgcggggg aaattcatcc ggcggcagat
    19261 aacaactggc gtttagcacc gatagctgcc gataagaaac tggatatccg tttcgaaact
    19321 tccccgtcag ataaagccgc agcgtttatt aaagagaaag ggcagtatcc gatgaataaa
    19381 gtcgcgaccg atgatatcgg gtttgcgatt tatcaggtgg atttgagtaa gtaaaacact
    19441 tctttttcgg cctataaatc aaccgcatcc ggcatttatt ggcggatgcg atgctgccgt
    19501 atcttatcca ccctacaagc catgaaccgt agaccggata agcgcagcgc atccggcagt
    19561 tatgccgcac gttcatcccg caccgccaac acctcgggca aattcaactc aatccagtcc
    19621 gccagtgcag caaccttttc gctcacctgc tcgcccagcg gcgtgaggct atattccaca
    19681 tgcggcggca ccaccggata cgcgatacgg ttaagaaaac catcctgttc taacgcctgt
    19741 aacgactgcg caagcatctt ttcactcacc ccaccgattt tgcgccgcag gtcgctaaag
    19801 cgatgagtac cttcgcgtag cgccaccaga atcaaccccc cccaacggct ggtgacgtgt
    19861 ttcaacacct cgcgcgacgg gcactgttcc gcaaagagat taccctcttt cagttgttgt
    19921 gacaggctaa cctgactcat ttcatactta cctttttgta cgtacttact aaaagtaagt
    19981 ttaggtgtta gcgtatttaa acacaagaca aaacgatgga gacttcccat gatcgctatt
    20041 actggtgcca ctggccaact tggtcactat attattgaat ccttgatgaa aacggttcct
    20101 gccagccaaa tagtggctat cgttcgtaat ccggcaaaag cccaggccct ggcagcacaa
    20161 ggcattaccg tgcgtcaggc tgactacagc gatgaagcca cactgacatc tgcacttcag
    20221 ggagtggaaa aactactgct gatctcttcc agcgaagtgg gtcaacgtgc cccgcagcat
    20281 cgtaatgtta ttaatgccgc aaaggcggct ggcgtgaaat ttatcgctta taccagcctg
    20341 ctacatgcag atacctcccc gctcggcctc gccgatgagc acatcgagac ggagaaaatg
    20401 ctggctgatt ctggcatcgt ttacaccctg ctgcgcaacg gctggtacag cgaaaactac
    20461 ctcgccagcg ccccggcagc actggaacac ggcgtattta tcggtgcggc gggcgatggc
    20521 aaaatcgcct cagcaacgcg ggcagattat gcgacagctg cggcacgcgt gattagcgaa
    20581 gccggtcacg aaggcaaggt ttacgaactg gcgggcgata gtgcctggac gttgacacag
    20641 ttagcggcag agctgaccaa acagagcggc aaacaggtta cctatcaaaa tctgagcgaa
    20701 gccgatttcg ccgcggcact gaaaagcgtc ggactgcccg acggactggc ggatatgctg
    20761 gcggattctg acgttggcgc atcgaaaggt ggtctgtttg ataacagcaa aacgcttagc
    20821 aaattgattg gccgcccaac gacaacgtta gccgaaagcg taagccatct ttttaatgtt
    20881 aataactagt taattaaagt ggcatcctcc cgcatcctct ctgataatga cgggatgccg
    20941 ggagcaatca tgtctgcttc ctgaacgttt cttctgacag aacaatggat gccagtaatg
    21001 attagcggcg tgctgtacgc cctgttagca gggttgatgt gggggcttat ttttgtcggg
    21061 ccgttgatcg tgccggaata cccggcgatg ttgcagtcga tggggcgtta tctggcgtta
    21121 gggttaattg cgctacccat tgcctggctg ggacgcgtgc gtctgcgtca gttggcgcgt
    21181 cgggactggc ttaccgcctt gatgctcact atgatgggca acctcattta ttacttctgc
    21241 cttgccagtg ccattcaacg tactggcgcg cctgtttcca cgatgattat cggcaccctg
    21301 ccggtggtga ttcctgtctt tgccaatctg ctttatagcc agcgcgacgg caaactcgcg
    21361 tggggaaaac tcgccccggc actgatttgt attggcatcg gcctggcgag tgtgaatatt
    21421 gctgagttaa accacggact ccccgatttt gactgggcac gttatacctc tggcatcgtg
    21481 ctagcgttag tttccgtggt ctgctgggca tggtatgccc tgcgcaacgc ccgctggctg
    21541 cgggaaaatc ccgacaaaca tccgatgatg tgggcgacgg cgcaggcgct ggtcacgctg
    21601 ccggtttctc tcatcggcta tctcgtcgcc tgttactggc tgaatataca aacgccggac
    21661 ttctccttac cttttggccc ccgtccgctg gtgtttatta gtctgatggt tgcgatagcc
    21721 gtgctttgct catgggttgg cgcactctgc tggaacgtcg ccagccagcg attaccgaca
    21781 gtgattctcg ggccgctgat tgttttcgaa acgctggcag gtttgctgta caccttttta
    21841 ctccgccagc aaatgccgcc gctaatgacg ctgagcggta tcgcgctgtt agtgattggc
    21901 gtggtcatcg cggtcagagc aaaaccagaa aaacctttaa ctgaatctgt ctcagaaagt
    21961 tgacatgctg gcagtgagtt aaataagcct ctgctacgta agggttatag cttttgcctt
    22021 aaaggtgtat ttaaaataca tcttatctta ttaagaatga ggtatcagct atggcttatc
    22081 gcgaccaacc tttaggtgaa ctggcgctct ctatccctcg cgcttcggct ctgtttcgta
    22141 aatatgatat ggattactgc tgtggcggta agcagacgct ggcgcgcgcg gcggcacgta
    22201 aagaactgga tgttgaggtc attgaagctg aactggcaaa gcttgctgaa caaccgattg
    22261 agaaagactg gcgtagcgcc ccgctggcag aaatcattga ccatatcatc gtgcgctacc
    22321 acgatcgtca ccgcgagcag ctgccggagc tgatcctgca agcgactaaa gtcgagcgcg
    22381 ttcacgccga caaaccgagc gtgccaaaag ggctgacaaa atacctgacc atgctgcatg
    22441 aagagctttc cagccacatg atgaaagaag agcagatcct cttcccgatg atcaaacaag
    22501 gcatgggcag ccaggcaatg gggccaatca gcgtaatgga aagcgagcac gatgaagcgg
    22561 gcgaactgct ggaagtgatc aaacacacta ccaataacgt cacaccgccg ccagaagcct
    22621 gcaccacctg gaaagcgatg tataacggca ttaatgaact gattgatgac ctgatggatc
    22681 acatcagtct ggaaaacaat gtactgttcc cacgcgccct agcgggtgag tgatctaaaa
    22741 gctggatggc attgcgccat ccagcatgat aatgcggtta tttccgcagt tcagcagccc
    22801 gcttcttacc aataaacagc cagcccaacc ccagcgcgat aaaccacagc ggggtaacca
    22861 gcagcgcctg gcgagtgtca tcttccagtg tcagcaacac gaccacgaac acaaagaacg
    22921 ccatacatac ccagcacatc agcttgccga gcggcatctt gtagatcgac ttttcatgca
    22981 gatgaggacg ctgtttgcgg tacaccaggt acgagcaaag gataatcgtc cagacgaaca
    23041 taaacagaat cgcggaaacg gttgtaatca tcgtgaacgc gccaatcacg ctaggattca
    23101 catacaacat caccacgccg ccgagcagac agatacacga gaaggtcaac cctttcgcgg
    23161 gtactgcgcg cttagaaagt ttagcgaacg ctttcggcgc aacaccttcc tgcgccagac
    23221 caaacagcat acggctggta gagaagacac cgctgttagc ggaagacgcc gcagaggtca
    23281 gcaccacaaa gttgatcacg ctggcagccg caggcaaacc taccaacaca aacagctcaa
    23341 cgaacgggct tttctccggg actaccgaac tccacggtgt cacggacata atcacaatca
    23401 gcgcgaagac gtagaacata atgatacgga tcggaatgga gttaatcgcg cgtggcagtg
    23461 atttctccgg atctttggtt tccgcagctg ttgtacctac cagctcaatc cccacgaaag
    23521 cgaaaaccgc tatctggaat ccggcaaaga agccacttaa acctttcggg aaccagccgc
    23581 cgtcattcca caaatgcgcg aatgacgctt ccacaccggt cggtgactga aagtgcatcg
    23641 ccaccatgac caggccgacg acaatcagcg acacgatggc gacgattttg atcatcgcaa
    23701 accagaactc catctcaccg aacattttca cggtggcaag gttgagcgtc agcagcagca
    23761 ctatcaccgc cagcgaggcg acccagtcgg agagatcggg gaaccagaac tgagcataag
    23821 ccgtgatcgc caccacgtct gccataccgg ttacaaccca gcagaaccag taagtccagc
    23881 cggtgaaata tcccgcccac ggccccagta aatcggaagc gaagtcacta aaagatttgt
    23941 attccagatt cgaaagcagc aattccccca ttgcccgcat cacgaaaaag agcataaaac
    24001 caatgatcat ataaacgaaa atgatcgacg gcccggcaag gctaatcgtt ttgccggacc
    24061 ccataaacaa cccagtacca atggcaccgc caatggcaat aagctgaata tgtcggtttg
    24121 tgagattgcg ccgtagcgac tgttcagccg gagcctgatc atcggcaacg acttttacct
    24181 gatctaccat gttttttctt cctgtacctg tctgtgttgt tcaggctcta cggcctttag
    24241 tcagtctatg atacgacgaa tctgtatcgg gactcatcga tattaggtaa caatacgcgg
    24301 gatgaatact aagatttaga tctaatgtta attttatgtt taaagtgagt gtcatatcac
    24361 ggtgataacg cgaaacagct cacaaaaata cacgcttacg gggtctttgc aacacaaaat
    24421 agcgccgttt ggcaaactga agggtttatt gctgaatgcc tgctcccctc tggttcgagg
    24481 ggaacagaat gcgctgctta gaggatttcc agcagttcga cttcaaacac cagggtgctg
    24541 aacggaggga tggatgcacc tgcgccgcgc tcgccatatg ccagttcctg cgggatagtc
    24601 agttcccatt tagaacctac cggcatcaga gtcagtgctt caatccagcc agggatcacg
    24661 ccattaaccg ggaattcagc gggttcacca cgagcaacgg agctgtcaaa cacggtgccg
    24721 tcgatcagtt taccggtgta atgaacacga acgcggtcgg tacgtgccgg aattgcgcct
    24781 tcaccctggt tgatcacgcg gaattgcagg ccagattcgg tgctattcac accttctttt
    24841 ttggcgtttt cttccaggta tttcacacct tcagcagcca tcgcctggaa acgctgacga
    24901 cgaacggcat cggcgcgttc gtggatttca cgcagcgcgc gatgcaccac atcaaccggc
    24961 acagccggat gtttgccttc cagcgcatcg gcaatacctg caaccagtgc ttctggcagc
    25021 agcccttcca ggccagattc actcagttgt tgccctacct gcaaaccaat gccgtagctt
    25081 gcttgcgctt cgatggtgtc aaaagttggg gtggtcatcg tatttccttt cttagggtat
    25141 aaaagtagcg ggcagcatat cagccacact tctttgggta aaactttgtc gcaggaatga
    25201 tgacaaatcc cggggaaaaa gaaacaatag caatatcccg tatgaatata ataaaggcgg
    25261 gtttaccacg gacatcatag cgttagctta ttcataggct atgattgagg aacaagacgt
    25321 ggagcaggag gaaaccatgc ccgggcgctt tgaactaaaa ccaaccctgg agaaagtctg
    25381 gcacgcgctg gacaattttc gcttcatgga cccgctgccg ccgatgcatc gtcgggggat
    25441 cattattgcc gccattgtat tggtggtcgg atttctgctt ccatctgatg atacgcccaa
    25501 cgcgccggtg gtgacgcgtg aagcgcagtt agatattcag tcgcaatcac aaccgccgac
    25561 agaagaacag ccgcaaacta cccaaacgca accattccag ccagatagcg gaattgataa
    25621 tcaatggcgc tcttaccgtg tcgaacccgg taaaacgatg gcgcagctgt tccgcgacca
    25681 cggactgccc gctaccgatg tctatgcaat ggcgcaggta gaaggcgcgg gtaagccgct
    25741 gagtaatttg caaaatgggc agatggtgaa aatccgccag aacgccagcg gcgtggtgac
    25801 gggcttaacc attgatacgg ggaataatca acaggtcctg tttacccgcc agccggatgg
    25861 cagttttatt cgtgcgcggt agatgaaggg tcaaccaaaa gccagctaag gattgtctct
    25921 ttgatgacct tacgtctggc ttccagtgcc tggggggaaa ccatactgat atgataaacc
    25981 aggccaagcg tatgctggtt gatcaaataa tatcccccaa gcgcagcaat attaatattc
    26041 acctgcaatg ggtcgatacc tggcttaaaa atattgtatt tcttcccttc atccaataag
    26101 gattccatta actgcaagtg cgcatggtta atttctaaga gacgctgtga ttttgcataa
    26161 tgcacacctt tactttgatt ttcactatgc acaattttta aaaaccaagg attggcaata
    26221 taataatccc atgtaaaatc aataactttc tcaatggctt tttgtggcgg aagatctgag
    26281 aaatcaaggg ccttttcttt ttgtcgaata tcgttccagg tatattccag cacttcgata
    26341 aataaatttt ctttattacc aaagtggtga taaaccgtct gtttactaca ccctgctgcc
    26401 tttacaatat tatctactct ggccccttca taaccatatt cggcaaattc attaaccgca
    26461 catagcagca atttttcctt caggctctgt acgttgctgg tatcaatata cataacgctt
    26521 ccccacgacc tcttcgtggt gactatcata acctgatttg atttcagctt cagaaaaaag
    26581 ggcaaaaact tgtactttca tcaggttatc aaatgtcctg tgatcgccat cgcatatcaa
    26641 ccaactagat ggttgatctt tttatcctcc agtcgcaagc gaatcatcac caaggaaaca
    26701 aaattcaaaa aagagagcta ttgtatgaaa tccactcgtc ctgttgccgt gattaccgga
    26761 gccgctcgcg gtattggaaa gggctgtgcg caggagcttg ctcgtggcgg gtttaacctg
    26821 ctgattaacg atcgtcccga tgccgacagc gtagaaaaac tgcatatcac acaacaagaa
    26881 tgtttggcgg aaggcgtgga agtgatttgc ttccctgctg atgtcggtga tctatctctg
    26941 catgaggaga tgctcgacgc ggcgcaaaac cagtgggggc ggctggactg tctgctcaat
    27001 aacgcgggga tttcagtgaa aaaacgcggt gacctcctcg acctcgaacc agacagcttc
    27061 gatcaaaaca ttgccattaa cacccgcgcg ccgttcttcc ttgctcaggc attcagtaag
    27121 cgcttactgg cacagccgaa accggaagcg gagctgccgc accgctcgat tattttcgtc
    27181 agttccatca acgccatcat gctggcgatg aatcgcggcg aatacaccat tgccaaaacc
    27241 gccgtttctg ccgctgctcg tctgtttgct gctcgccttt gtaatgaaca aattggcgtt
    27301 tatgaagtgc gccctggtct catcaaaact gatatgacta ttccggcaac ggcttattac
    27361 gacgaactga ttgccaaagg tctggtgccg tggggacgtt ggggttatcc ggctgatatt
    27421 gcttccaccg tccgcgcgat ggcggaaggt aaactgattt acacctgtgg tcaggcggtc
    27481 gcaatcgacg gcggcttaag tatgccgcgc ttctgaggct gaatcatgag agatctgtta
    27541 caacgcccgg atttattcag tatcaatacc gcgacactgg gctataaaac gccgctaccc
    27601 gccattattg atgcctgcgc cgcacgcggt attggcgcaa ttgccccctg gcgcagggag
    27661 ttgcagggtg aagatctcca gcaaatcacc cgccagcttg ccgccagtaa catgagcgtt
    27721 tccggtttgt gccgtagcac ttactacacc gcgccgacac tggcggaacg caaactggcg
    27781 attgatgata accgccgggc gctggacgac gcagcggtgc ttaacgccgc ctgttatatg
    27841 caggtggttg gcggtctgcc aacgggcact aaagatctat atgaagcgcg agaacaagtg
    27901 aaacaaggga ttcgccagtt gctgccacat tcgaaagatg tcggcttgcc aattgcgctg
    27961 gagccactgc acccgatgac agctgccgat cgctcttgcc tgtgcacgtt acgtcaggcg
    28021 ctggactggt gtgatgaact cgatcccgat ggtgagtttg gcctcggcgt ggcggtggat
    28081 gtttatcacg tctggtggga tccggatctc gccagtcaga tcctgcgtgc cggaaaacgc
    28141 atcctcgcgt ttcatgtttc cgactggtta gtgccgacca ctgatctggt caatgaccgt
    28201 ggtatgccgg gagatggcgt catcaatatt ccgtcaattc gtcggctggt tgaaaacgcc
    28261 ggatttaatg gtgctattga actggaaata ttctcgcctt actggtggca gaaagatatt
    28321 aacag
//
LOCUS       DBKEKI010000048        27364 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.048, whole genome shotgun sequence.
ACCESSION   DBKEKI010000048 DBKEKI010000000
VERSION     DBKEKI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.048"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..707)
                     /gene="pdxK"
                     /locus_tag="ACUQPX_001980"
                     /gene_synonym="thiJ"
     CDS             complement(<1..707)
                     /gene="pdxK"
                     /locus_tag="ACUQPX_001980"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HHV9056702.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLF"
     gene            812..1186
                     /locus_tag="ACUQPX_001981"
     CDS             812..1186
                     /locus_tag="ACUQPX_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HHV9056703.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQMDKTVPAEGN"
     gene            1219..1942
                     /locus_tag="ACUQPX_001982"
                     /pseudo
     CDS             1219..1942
                     /locus_tag="ACUQPX_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(2146..3057)
                     /gene="cysM"
                     /locus_tag="ACUQPX_001983"
     CDS             complement(2146..3057)
                     /gene="cysM"
                     /locus_tag="ACUQPX_001983"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HHV9056704.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMLELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(3191..4288)
                     /gene="cysA"
                     /locus_tag="ACUQPX_001984"
     CDS             complement(3191..4288)
                     /gene="cysA"
                     /locus_tag="ACUQPX_001984"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HHV9056705.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(4278..5153)
                     /gene="cysW"
                     /locus_tag="ACUQPX_001985"
     CDS             complement(4278..5153)
                     /gene="cysW"
                     /locus_tag="ACUQPX_001985"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HHV9056706.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(5153..5986)
                     /gene="cysT"
                     /locus_tag="ACUQPX_001986"
     CDS             complement(5153..5986)
                     /gene="cysT"
                     /locus_tag="ACUQPX_001986"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HHV9056707.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYIWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGLEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(5986..7002)
                     /gene="cysP"
                     /locus_tag="ACUQPX_001987"
     CDS             complement(5986..7002)
                     /gene="cysP"
                     /locus_tag="ACUQPX_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HHV9056708.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(7306..8097)
                     /gene="ucpA"
                     /locus_tag="ACUQPX_001988"
     CDS             complement(7306..8097)
                     /gene="ucpA"
                     /locus_tag="ACUQPX_001988"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HHV9056709.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(8226..9083)
                     /gene="murR"
                     /locus_tag="ACUQPX_001989"
     CDS             complement(8226..9083)
                     /gene="murR"
                     /locus_tag="ACUQPX_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HHV9056710.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            9247..10143
                     /gene="murQ"
                     /locus_tag="ACUQPX_001990"
     CDS             9247..10143
                     /gene="murQ"
                     /locus_tag="ACUQPX_001990"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HHV9056711.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTELMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            10147..11571
                     /gene="murP"
                     /locus_tag="ACUQPX_001991"
     CDS             10147..11571
                     /gene="murP"
                     /locus_tag="ACUQPX_001991"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HHV9056712.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            11576..11662
                     /locus_tag="ACUQPX_001992"
                     /pseudo
     CDS             11576..11662
                     /locus_tag="ACUQPX_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11751..12650)
                     /gene="yfeX"
                     /locus_tag="ACUQPX_001993"
     CDS             complement(11751..12650)
                     /gene="yfeX"
                     /locus_tag="ACUQPX_001993"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HHV9056713.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12746..13321)
                     /locus_tag="ACUQPX_001994"
     CDS             complement(12746..13321)
                     /locus_tag="ACUQPX_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HHV9056714.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13382..13831)
                     /locus_tag="ACUQPX_001995"
     CDS             complement(13382..13831)
                     /locus_tag="ACUQPX_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HHV9056715.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(13818..14243)
                     /locus_tag="ACUQPX_001996"
     CDS             complement(13818..14243)
                     /locus_tag="ACUQPX_001996"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HHV9056716.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            14457..15326
                     /gene="amiA"
                     /locus_tag="ACUQPX_001997"
     CDS             14457..15326
                     /gene="amiA"
                     /locus_tag="ACUQPX_001997"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HHV9056717.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKQKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVINYF
                     HWFDNQKAHSKKR"
     gene            15330..16229
                     /gene="hemF"
                     /locus_tag="ACUQPX_001998"
     CDS             15330..16229
                     /gene="hemF"
                     /locus_tag="ACUQPX_001998"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HHV9056718.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16235..17287)
                     /gene="eutR"
                     /locus_tag="ACUQPX_001999"
     CDS             complement(16235..17287)
                     /gene="eutR"
                     /locus_tag="ACUQPX_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HHV9056719.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVYQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDGAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(17333..17833)
                     /gene="eutK"
                     /locus_tag="ACUQPX_002000"
     CDS             complement(17333..17833)
                     /gene="eutK"
                     /locus_tag="ACUQPX_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HHV9056720.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLTACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(17846..18505)
                     /gene="eutL"
                     /locus_tag="ACUQPX_002001"
     CDS             complement(17846..18505)
                     /gene="eutL"
                     /locus_tag="ACUQPX_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HHV9056721.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPQHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTSGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(18515..19402)
                     /gene="eutC"
                     /locus_tag="ACUQPX_002002"
     CDS             complement(18515..19402)
                     /gene="eutC"
                     /locus_tag="ACUQPX_002002"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HHV9056722.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(19423..20784)
                     /gene="eutB"
                     /locus_tag="ACUQPX_002003"
     CDS             complement(19423..20784)
                     /gene="eutB"
                     /locus_tag="ACUQPX_002003"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HHV9056723.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            20972..22180
                     /locus_tag="ACUQPX_002004"
     CDS             20972..22180
                     /locus_tag="ACUQPX_002004"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9056724.1"
                     /translation="MSKTPLTAKAIEAAQPQDKPYKLTDSMTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNAI
                     ASFKAVAEDWMATKVSGWSESYTKQVRSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVNRNPVADLDTALKARRPGHNAWIPINEIPA
                     FYKALERAGSVQIQTAILLLILTALRTAELRLMRWEWVDLQSATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSAFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYSPDWIEIQLAHVPKGVRGVYNQAAYLKQRRAMMQDYSDCIDSIL
                     AGDGNPLEPE"
     gene            22426..23013
                     /locus_tag="ACUQPX_002005"
     CDS             22426..23013
                     /locus_tag="ACUQPX_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056725.1"
                     /translation="MNEIKKPGKAATSSGRYEKQAKGEFLMSDMLAVEQETNNDVRQY
                     LNKINELRNKAPKNEETKHEEHTPDHYEAKQQEQAWRGNLRYLDTLNRLDEVLPRKLY
                     ERWEKEHTVNDEAVLRALCYFAGTGKNSQLGWCRVGRGTIDKRARLSKNTVKKCLDRL
                     VHHFKLVERTEGYIPGSAERECNEYQLLFKPYNMK"
     gene            24486..24677
                     /locus_tag="ACUQPX_002006"
     CDS             24486..24677
                     /locus_tag="ACUQPX_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9056726.1"
                     /translation="MNDSELMTVNEVAALFGVTRRTIFRWMNKIKGWPVPVSPIDSRI
                     KFIRSEILEFYKNKGARHQ"
     gene            24754..25080
                     /locus_tag="ACUQPX_002007"
     CDS             24754..25080
                     /locus_tag="ACUQPX_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056727.1"
                     /translation="MKIKANTIKEITSIKVKGADAMHSLDSYFQRTTAPKSAAQERRE
                     EFQEKVMRSADYIADKFVETVRPLVDEVADKLQSEMPEDMEGTAKARLLFELSRRFGV
                     SISTFK"
     gene            25098..25640
                     /locus_tag="ACUQPX_002008"
     CDS             25098..25640
                     /locus_tag="ACUQPX_002008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056728.1"
                     /translation="MDAFDVLTNKGDELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIEIIKPQIPQIVADAIKAAGLEKRIAGLKTNVEKMEMHKQSGAPTPDEYFSPD
                     AEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     AADPEAEQRKRVFNAIGIRY"
     gene            25779..26066
                     /locus_tag="ACUQPX_002009"
     CDS             25779..26066
                     /locus_tag="ACUQPX_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056729.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            26105..26311
                     /locus_tag="ACUQPX_002010"
     CDS             26105..26311
                     /locus_tag="ACUQPX_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056730.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            26623..27265
                     /locus_tag="ACUQPX_002011"
                     /pseudo
     CDS             26623..27265
                     /locus_tag="ACUQPX_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816255.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
BASE COUNT         6878 a         7236 c         6807 g         6443 t
ORIGIN      
        1 caaaacaggt cgccagtccc tttcaggtcg gtttttaccc gtgaatggga aatgacattc
       61 acgctgtcgg cactgaccac caccacctgc atctcctgat tttcttcatt accggaggcg
      121 ctggtaatca ccacccattt taatgtgtct gaaagcagac tttttgcggc agcaatggca
      181 ctgtcgagat cgcggcaatt tttaccggtc aggatttcca gctcaaagat attgggggta
      241 attccctgcg ccagcggcag taaatattgt cgatacgcct cgggaaggtc aggtttgaca
      301 taaattccgc tatcaatatc gccaatcacc ggatcgacca tgatcaatag gtcaggatgg
      361 tctttgcgta gcgcagtcag ccactcggca aggattttga tttgcgatgc cgttcccata
      421 tagcccgtgg ttacagcacg aagttggcgc agcgcatcac gctcctgaag cgcacgcaaa
      481 tagccgctaa accattcgtc cggaatcgca ccaccgtaga aagtgtcata atgcggcgta
      541 ttgctcagca ataccgtcgg cacggcaaag acattcaagc cgttctgttt tatagcaggc
      601 accgcaatgc tgttgcctac gcttccgtac actacctgcg actgcacggc gacgatatcc
      661 gcctgcagcg ccctactctt atcgttaaac aacaacaaac tactcattta attttttctc
      721 cttgccgatg atcctcatcg tattccaacc gaaactttac ctgattctgg cagtcaaatc
      781 ggctattaca aaacaaggat aaggtaattc agtgaagaaa atcatttgtc tggccattac
      841 gctgctaatg acactccccg cttacgcgaa gttaactgtt catgaagaag cccgcatcaa
      901 cgccatgctg gagggattag cacagaaaaa ggatttgata tttgtgcgca acggtgatga
      961 acatacctgc gatgaagcgg tttcccacct gcgtctgaag ctcggcaata cccgtaaccg
     1021 cattgacact gccgagcagt ttattgataa ggtagcttcg tcgtcatcga ttaccgggaa
     1081 gccgtatatc gtgaagatcc ccggtaagag cgatgagaac gcacagcctt ttttacatgc
     1141 gttaattgcg cagatggata aaacggtgcc tgcggaagga aattaatccg ctttggtaag
     1201 gcatttacag gaggtaacat gaaaaaatgc tttatttgtc acgatgaaaa atcgaataaa
     1261 ttttggtgga tagattacga aggggatagt ttagctgtca actatggcaa ggtaggtagt
     1321 attggtaaat tccatacaaa agaattcgat aatgaagaac agtgtctgaa agaagccagt
     1381 aaattgattg ccgcaaaaat gaaaaaagga tatcaagaaa ctccagactt taacttcatg
     1441 gatctctact attttgatga tgaagagatt gggttacatc ttaaaacgtc acacccaaac
     1501 ttccagtgcc attttactga tccactttat atgtgttgct gggatgaaga atctcctttt
     1561 ggtagcgatg aaggtgctga tgctctaaac gaacttgaaa atagcctccg ttctggactg
     1621 tgctgatttc cctcaaatgt taattgaaac tatgtggggt atgaaataca tcgctatgga
     1681 cagtattctt gaagaggatg tgcgtgcgca attactagcc gatgaaatga gctctatcca
     1741 gagcaatatg attacctacg cgactgcatt cggtcagatc aaagtcatgg gtaaaatctc
     1801 ccataaactt aaaaagatgg gactcaatgc actagcgcgt catcagctta ccgcaaaaat
     1861 tcttcaatgg ggtgacggtc aggactcacc aatacttcaa aaaatgattg atgaccttac
     1921 tgcttttcca cacgaaaatt aaaaactgca tttgtcggca gcaacaactg ccgacaaatc
     1981 agattattga caaagtgcac tttgtttatg ccggatgcgg cgtaaacgcc tgatccggca
     2041 taaaaatatt gctaattcaa tatattgcag aaacattgta ggcctgataa gcggagcgca
     2101 tcaggcagtt ttgcgtttgt catcagtctc caatgctatt aatccttaaa tccctgcccc
     2161 ctgactaaaa tgctcttccc caaacactcc ggtggagagg tagcgatcgc cacgatcgca
     2221 gatgatcgct accaccaccg caccagggtt agcttttgcc acccgcagtg ctccagcaac
     2281 cgcgccgcct gagctgacgc cacagaatat tccttcccgc accgccagtt cgagcatggt
     2341 gttttccgca tcgcgctgat gaatatccag cacctcatcc accagcgaag cgttgaaaat
     2401 ccccggcaga tattccgcag gccagcggcg aatgcccgga atactgctgc cttcttccgg
     2461 ttgcaggcca acaatggtca ccggtttgga ttgttcgcgc ataaagcgtg aaacgccggt
     2521 gatagtgccg gtcgtcccca tgctggagac aaaatgagtg atgcgcccgc cggtttgctg
     2581 ccagatttcc ggcccggtgg tggtgtaatg cgcataaggg ttatcgggat tattgaactg
     2641 atcgagcagc tttccttcgc cacgattcgc catctccagc gccagatcgc gcgcaccttc
     2701 catgccctgc tctttggtga caagaatcag ttccgcacca taagcacgca tcgccgcacg
     2761 gcgttcctgg ctcatgttgt cgggcatcag caatttcatg cgatagcctt tcagcgcggc
     2821 aatcattgcc agcgcaatgc cggtgttacc actggtggct tcgattaaga catcacccgg
     2881 tttaatttcc ccgcgctttt ccgcctcgac gatcatcgaa agtgccgcac gatctttcac
     2941 cgaacctgcc gggttattgc cttccagttt taaccacact tcactgccgt tatccggccc
     3001 cattcgctgc aacttcacca gaggcgtatt gcctattgtt tgttctaatg tactcacgat
     3061 ctctatccat acgtggtgtt gcctgatgcg acgcttgcgc gtcttatcag gcctacaggt
     3121 tacaaacctt gccattaaaa agcccggatc gcgggaagcg cctccgggcg tttaacattc
     3181 actcaaccta tcaggcgctt tgtgcgagag caagttcctc atcgcgggtt tcaatacgct
     3241 cgtcgccgtt atacagccgc gcatgttgca gaccaacgaa taaacgctcg ccacgctgcg
     3301 gggcatcgtc gccatgcatc acgaccgtca gcggttcgtt gtaccacccc agcggctgca
     3361 ccactaattg ggtgtagtga cctttcgggc tggcttccag tacctgtacc ggcagcggcg
     3421 aatcgagact ggtacggcgg ctgatatcca cttcccacgg gcgcaggaag agatccaccg
     3481 gaccctgata cgcaggtgtg tagcccagcg gccagcgatg cgcgccaaca tggaactgcc
     3541 cgccgcgaat ggttccctgc aggcggttca cttcgcccat aaattcgagt acaaaacggg
     3601 tcgctggttc gcgccatacc tgattcggcg catcagcctg ttcaatattc ccctgactca
     3661 tcaccactac acgatcggct acttcggtcg cctcttcctg gtcgtgggtc acaaacacgc
     3721 tggtgaattt cagctcttca tgcagttgac gcagccagcg acgcagctct ttacgcacct
     3781 gcgcatccag cgcgccaaac ggttcatcaa gcagcagaat ttgcggttct acagccagcg
     3841 cgcgcgccag cgccacacgc tgtttctggc cgccggaaag ctgcgccgga taacgatccg
     3901 ccagatgcgc aagctggacc atttccagca attttgtcac tttcgctttg atggctgcgg
     3961 cattcgggcg ctcgcgacgc ggcagcaccg tcaggccaaa agtgatattg tcgaataccg
     4021 tcatatggcg gaacagcgcg taatgctgga acacgaaacc gactttacga tcgcgcgcgt
     4081 gcaggcgact cacgtcggtg ccgtggaagc gaatatgccc gctggtttga tgctccagcc
     4141 cggcgataat ccgcagcagc gtggttttcc cggaaccgga cggccccagc aacgcgacca
     4201 tctgaccaga aggaatatcc agtgagatat cgttcagcac ctgggtgcga ccaaaagact
     4261 tcttgatatt ggcaatctca atgctcatga tgctcctcct gctgcgcgcg tttttcctga
     4321 ttctccaagc gccactgcaa catactcttt aaaaacaggg tgataatcgc catcagcgtc
     4381 aacagcgcgg cggcggtaaa ggagccgacg gtgttgtagt cctgctccaa caattcaatc
     4441 tgtaacggca gcgaaagtgt ttcgccgcga atcgagccgg aaaccaccga cactgcacca
     4501 aactcgccaa tcgcgcgggc gttggtcagc accacgccgt aaagcagcgc ccagcgaata
     4561 ttcggcaatg tgacgcgacg gaacatctgc cagccggacg cgccaagcaa aatcgccgct
     4621 tcgtcttcct ggctgccctg gcttaacatc accggcacca gctcgcgcac cacaaacgga
     4681 cacgtcacaa agatggtgac cagcaccatt ccgggccagg agaacataat ttgcagatta
     4741 tgttcatcca gccagccgcc gagcgggccg ttggagccgt agaacagcag atacaccaga
     4801 ccggcaacca ccggcgatac ggcaaacgga atgtccagta gcgtcagcag taactggcgt
     4861 cccgggaagt taaagcgcgt caccagccag gccagcagaa tgccgaacac caggtttacc
     4921 ggtacggcaa tcagcgcgat catcaccgtc agccagatgg cgtgcagcat gtccggatcg
     4981 gccagattct gtaaaaccgg catcagcccc ttgctgaatg cctgcacgaa gatgtaaatc
     5041 atcggcacca gcaggatgaa cgccgaaacc agcatcccga tgccaatcag aaaccatttg
     5101 ccccagttaa tcgggcgcgc gtcataacgc ttcaattggg taacttccgc cattaatgac
     5161 ctaccacacg ccgaccaaag cgactttgca gagtgttaat tgagaacagc agcagcagag
     5221 atgccgcgag gatcaccgaa gcaatcgcgc tcgctgccgg gtaatcaaac tcctgtaagc
     5281 gcacaaaaat catcagcgac gtcacttccg tcttccacgc gatatttccg gcgataaaaa
     5341 tcaccgcgcc aaattcacca agactacggg taaacgacag cgccacgccc gccaccagcg
     5401 ccggagaaag ctccggcagc accactttgc agaaactctg ccagcgcgtt gcaccaagcg
     5461 tttccgccgc ttcttcatat tccaggccta actcttccag caccggctgc acggtacgca
     5521 ccacaaacgg aatgctggta aaggccatcg ccaccgcaat accaagccag atataggtga
     5581 ctttgatatc aaacttcgcc agccattcac cgtaaaaacc gtttacggaa aagagcgagg
     5641 ccagcgttaa accggcgact gccgttggca gcgcaaaggg taaatccatc agcgcatcaa
     5701 gcagcgtgcg gcccggaaag cgataacgtg ttaaaatcca cgccatcagc agaccgaaaa
     5761 cgccgttaaa aatcgatgcc acaaacgccg acagcagcgt cactttgtag gccgcgacta
     5821 cctgcgggtt ggtgatcacc tcccagtact gcgcccagct catctcagaa agctgcatca
     5881 ccagcgcaga gagcggcagc agcaatatca ggcacacaaa cagcagactg gtgccaaggc
     5941 ttaaagtaaa gcccggcagc acgcgtcttg aagaaactgc aaacattact tacgccccgc
     6001 cgctaacagc ttgtctaact cgccgccgct ggtgaagtgg gttttcatca cttccggcca
     6061 tgagccaaat ttgtcttcca cgcggaacag ttcggtctgc gggaatttat ctttcagttt
     6121 gtccatgact tccgggttat tcacacggta gtaatagtcg gtgatgatgg tctgcgcctg
     6181 tgggctgtag agccagttca ggtaggcttt tgcggctttt tccgtaccgt tggcctgcac
     6241 atttttatcg acccacgcca ccgggaattc cgccagaata ttggttttcg ggatcaccac
     6301 ttcaaagccc tgtgcttcat actgtttacg gatgttgttc acttccgatt caaagctaat
     6361 cagcacatcg cccaagccac gctcggcaaa agtggtggtt gcgccacgac cgccggtatc
     6421 gaacacttca acgtttttca ggaactgggt cataaactgt tcggttttgg ctttatcgcc
     6481 accgtcagct ttatccgctg cgccccatgc cgccagatag gtataacgcg cgttacccga
     6541 cgttttcggg ttcgggaaaa tcagcttcac gtcggagcgc accaggtcgt tccagtcgtg
     6601 gatattcttc gggttgccct tacgcaccag gaagcccatg gtggagtaga acggcgagct
     6661 attattcggc aggcgcgact gccagtcagc cgggatcagc ttgcctttgt cgtgcaggat
     6721 ttgtacgtcg gtcacctggt tataagtgac aacgtctgct tttaagccct gcaaaatcgc
     6781 cagcgcctgt tttgatgacc cggcatgaga ttgttttatc gtcagtttgt cgccgccgtt
     6841 atcttttgcc cattgctgtt caaacggcgg attcagggcg gcaaacagct cgcgggagac
     6901 gtcataagaa ctgttcagca gttccgttgc ctgtacatgg cccgccagca gcagagaagc
     6961 gaccagcgcg agtgagttct ttttcagtaa gttaacggcc attgcgcacc cttataaatt
     7021 taatgacttt ctaatagcca tcatatttat aacggacgtg aaaggagtaa cggttttata
     7081 taccgtttgg tgatttggaa gttaaaaagg gaataagaac ttggttcaac aggcgtaatt
     7141 gttgcaggca gtttggacac ggacagcgcg gagaaaccgg agcgtacaca tagtacgtga
     7201 ggatttcgag cactgcccgg ggccaaaatg acaaatgaaa tagcctggtg aacttagttc
     7261 aagacgaaat cctccccaca catgcaggga ggaaacagag gtgaatcaga taccgacgct
     7321 aaccgtctcc ggcagtgtgc tgccgccatc aatcacattc tgtgtaccgg ttaaatagct
     7381 ggattcatcc gatgcgagga aggccgccag ttcgccgact tccagcggat cggcgaggcg
     7441 acacagcggg attgcttttg ccatttcagt cagcaccgat tctggatctt ccgggttcga
     7501 ctggcgggca atgctttccg ccattggcgt gcggacgtat cccgggcaga tggcgttaac
     7561 gcgaataccg gattgcgcgt actccaccgc cagcgattta gtcaggccaa caatcgccgc
     7621 tttcgttaag gcatacgccg tttcgccagg atcggccacc atatcaccag tgactgaaga
     7681 catcatcaca atgcgaccat ctttgcgcgc aatcatctcc ggcagcaccg ccttcgtgac
     7741 gttccatacg cctttaatat tgatatcaat atggaaatcg cgatcttcat cgctcatatc
     7801 gaggaaactg cccagacgac aaacgcctgc gttattcacc aggatatcaa tgcgcccttc
     7861 tttttccttc gcgcgtttga tagctgcggc taccgacgcc gggtcacgca catcggcgac
     7921 aaccgccgta cagcgatgac cacgaccaca cagttcgtcc gctagctttt cgatctcagg
     7981 ggagatatcc agcaagatta ggttcgcgcc atgacgtgca aaagttctgg caattccttc
     8041 gccaattccc tgcaatgcgc ccgtaatcag tgctgtcttg cccgtgagtt tacccatttt
     8101 aatgctctcc ttgttaatac agagcgttac actccattcc ctctgaaaat acaggataaa
     8161 caaaatattg ctgcggatca gctcactaat tcaggaaaat ctcaatccag tcgctgctct
     8221 atgcatcatt tcaggcgttg tgttagctca ctgctgcgct gaatcatttt caatgacgcc
     8281 acatcattga gttgcacaag accaacaaac agtaaatcgg taacagaatt ttgcgccgtg
     8341 cgggtagaca tcgacgagct acgccattcg gtttcaccgg aaacggtatc gagcgtgaaa
     8401 tgcgccagcc gccgcagcgg agagtcagcc agagaagtaa tggcaatcac cgttgccccc
     8461 tgctttcgtg ccgcttctgc gcacaagaca atctcttttt tactgccgct gtaagagata
     8521 gcgatttgta catcgccttt tttcaacgcc tgggaaacgg tggcttgtac gtgcgtgtcg
     8581 gcttcgcagg ccacgcgata gccaattttc atcagtttga acgacagatc gcgccccacc
     8641 agcgccgatc cgcccaggcc cgttatctgg ataaacggtg ctttactgat gacctcaatg
     8701 attttttgca gccgcgcgta gtcgaacaac gcacaggttt gctccagcgc cagctctttt
     8761 tcgcgattca gtttgcgggc aatcacttcc agcgaatcat cactggtaat cgaactgtgc
     8821 aggtgcagcg ccgtggcatt cgttttctcc cggctggcac tgtattcgcc aattaacgcc
     8881 atacgcaatt cagtaaatcc ttgcgcgccg agcttctggg caaatttcac aatgctcgac
     8941 tggctgatac cgagctgttt cgccatctgc cgtgaagaga cggatttcag ctcgctcact
     9001 ctggcccgca gaaagtcagc aattttttgc tcgttcccgg taaattcact ttccgcattg
     9061 cgtattttcg tcaggtacaa catcttccac tctcctgggc tggcagcaaa aattccgctt
     9121 aagttcttat gacgctcttc acactctgcg agtgtagact caatgattcc tttaagtctg
     9181 tcaattcggg atatatgatt ccatacatct gcaattattt gatcgtaaat agtaaggtca
     9241 ccaccgatgc aactagaaaa gatgattact gaaggctcga acaccgcctc ggctgaaatt
     9301 gaccgcgtat cgacgctgga aatgtgccgg attatcaacg atgaagataa aaccgtaccg
     9361 cttgccgttg agcgcgtcct gccggatatc gccgcggcga tcgatgttat ccacgcccag
     9421 gtcagcggcg gcggtcgtct gatttatctc ggtgcgggaa catccggtcg tctggggatt
     9481 ctggatgcca gcgaatgtcc gcccacctac ggcgtgaaac cgggtctggt ggttggtttg
     9541 attgctggag gcgaatatgc cattcagcac gcggtggaag gcgctgaaga tagccgggaa
     9601 ggtggtgtta acgatctgaa aaatattaat ttaacggcac aggatgtggt ggttggcatt
     9661 gctgccagcg gtcgcacgcc gtatgtgatt gccggactgg aatacgcacg ccagctcggc
     9721 tgccgcacag tgggaatttc ctgtaatccg gggagcgccg tttcaaccac cgctgagttt
     9781 gccattacac cgattgtagg tgccgaagtt gttaccggct cttcgcggat gaaagcaggt
     9841 acagcgcaga aactggtgct caatatgctt tccaccgagc tgatgattaa atccggcaaa
     9901 gtgttcggca acctgatggt cgatgtggtc gccaccaacg aaaaactgca tgtgcgccag
     9961 gtcaatatcg ttaaaaacgc caccggatgt aatgccgaac aagcggaagc ggcgttaatt
    10021 gcctgcgaac gcaactgtaa aacggccatt gtgatggtgc tgaaaaatct cgatgccgca
    10081 gaagctaaaa aacgcctgga tcaacacggc ggctttattc gtcaggtttt agacaaggaa
    10141 taacccatgg ccaaagagat cagcagtgaa cttctgaaca ccattcttac ccgtgtcggc
    10201 ggaccgggaa atatcgccag ttgtggtaac tgtatgacgc gcctgcgtct gggtgtacat
    10261 gacagttcac tggttgatcc caatatcaaa acgctggaag gcgtgaaggg cgtcattttg
    10321 accagcgacc aggtgcaggt cgtttttggg cctggtaaag cgcatcgtgc cgcaaaagcg
    10381 atgagcgagc tgctgggcga ggcaccggta caggatgccg cagaaatcgc cgcccagaac
    10441 aaacgtcagt taaaagccaa acaaacctcc ggcgtgcaac aatttctcgc caaattcgcc
    10501 accatcttca cgccgctgat ccccggtttt attgccgccg gtctgctgct ggggatagcg
    10561 acgttaattg ccacggtgat gcacgttccg gcagatgctc agggcacgct acccgacgcg
    10621 ctgaatttta tgaaggtgtt cagcaaaggt ttgttcacct tcttggtgat tctggtgggc
    10681 tataacgccg cccaggcatt tggcggcacg ggcgtcaatg gcgcaattat tgccgcactc
    10741 tttttgctcg gttataaccc cgctgcaacc accggttact acgccggttt tcacgatttc
    10801 tttggtctgc ccatcgatcc gcgcggcaat attatcggcg tgctgattgc cgcgtgggcc
    10861 tgcgcccgca ttgaaggcat ggtgcgccgc tttatgccgg acgatctcga catgctgctg
    10921 acctcgttaa tcaccctgct gatcaccgct acgcttgcgt acctgatcat tatgccgctg
    10981 ggtggctggt tattcgaagg tatgtcatgg ctgtttatgc acctgaacag taatccgttc
    11041 ggttgtgcgg ttttagccgg gctgttcctg atcgccgtgg tatttggcgt gcatcagggc
    11101 tttattcctg tttacctcgc gttaatggac agccagggat tcaacagctt atttcccatc
    11161 ctttcaatgg caggcgcggg ccaggtgggc gcggcgctgg cactctactg gcgggcgcaa
    11221 ccgcacagtg cgctacgcag tcaggtacgc ggggcgatta ttcccggcct gctgggcgtt
    11281 ggtgaaccgc tgatttacgg tgtcaccctg ccccgcatga agccgtttgt taccgcctgt
    11341 ttaggcggcg cggcgggcgg tttgtttatc ggattgatag cctggtgggg tctgccgatg
    11401 ggcttaaaca gcgcctttgg gccgtctggt ctggtggcgc tgccactgat gacttccgca
    11461 caaggcatcc ttccggcaat ggcggtttat gctggcggga ttctggtggc atgggtttgc
    11521 gggtttattt tcaccacgct ctttggctgc cgtaacgtca atctggactg atattatgaa
    11581 acggacaatg ctctatcttt ctctgctggc tgttagttgt agcgtcagcg ccgcaaaata
    11641 ccctgttctg acagaaggta gttgatcaag tatatgcggc gttaaagcag taataaaaag
    11701 gccagtcagt aatgttactg cctggccttt aatcaatgaa tcagaaacgc ttacagcgcc
    11761 atcaacttgt ccagcgacgg tgcgaaataa tagccgccgg ttaccggttt ggtgaaacgc
    11821 aacatcgcat cacgtttgcc atcggtatcg ccaaacatgc tcagcagttg ctgctcaatg
    11881 ttatgcagac gcgcgcagta ggcgcagaag tacagaccgt gagtgccact ggcggtgccg
    11941 tacggcaggc tctggcgaac aatcttcagc cctttgccat cttctttcag atcaacgcgg
    12001 gtgaggtgag aggtttccgg acgttcgtcg ccgtcaatct cttcgttggc ctctttggtg
    12061 cgcccgatca tcatctcctg atcgtgaacg ctcatccggt tgagctgctt caggttgtgc
    12121 tcccaacgct gcacaaacac atagctgccg ccggcatcca cgccgtcttt gataaccgcc
    12181 acttcgcggc gcgtctcttc acccgccggg ttttctgtac cgtcaacaaa gccgctcagg
    12241 tcacgctctt ccacccaacg gaagccgtgg atctcttctt tcacttcaat gcagtcacca
    12301 aaggcttcca tcgccgcctg agcgacagag aagtttacgt cgtgacgcag agaaagaatg
    12361 tggatcaaca catcgaactg ggtcgtcggc gcaaggcctt taccgtagcc cggaaaatct
    12421 ttcagctctt ctgccccaac gccgccgctc agagcgcgcc aggtgttgtt accaaaggca
    12481 accaccgcac caagatgcgc gtccgggaat ttcgcttcaa aagttgccag tttgtcggca
    12541 aatgttttac tggccgcacg cagggcgtca acttcccctt tcacgttggc ttcgatccaa
    12601 atcgccgcgc ggcaatgttc tggcaaaatg ccactctgaa cctgagacat tgttcctcct
    12661 gaaaataaga atgccacgca atccgtggca ttgttgagcg ctattttacg tgttttttcg
    12721 cgacggcggt ttgttcagac gcaaattaac gccgccagat aattttactg actttccagt
    12781 ttttcagggt atcgtccgaa ggcattaacc cttcaggacc acgccattcc ccgctgaact
    12841 ggtagctaat atgttgactg ccttcggctt tacattcgac ggcacgatta tcatcaccat
    12901 cagctttttg gcaattgcca aatgctttgc tgtaaaggtc gctaaacggt gtaccgattt
    12961 taacaccggt gtcagcagga atatcgctat ccagcacgtc aatgcgactg atcgtgcctt
    13021 gatcgccgtt aatcaccatc gccacgttgt cgcctttcat cacttcaaaa aagcgcacca
    13081 cgttgccgtt cgcggttttc attccgctgc gcaggcgata atcgccatca agcgcatcag
    13141 caatggcttg ttcttgcagt ggtgtggacg ccgttaattc acccacgccc tgctcgctca
    13201 ctttggtgga cgatccaaac cagttccacg ggttagcggc agaccagtta actgacgaca
    13261 tcgtggaaca gccggtcagc atcaacggca tagcgcataa cattaaacgc agcgatttca
    13321 tgtcacttcc tttggttatt caataacgtt gcttggagtg caaattcacc aaaaagtgcc
    13381 gttattcttt ttcttcatta aaacaagcgc gtaaacgtcg attggtcagc agccagatta
    13441 acgccacaat atccgccact accagcgcca gaccgatacc gctaacggat tcaccgttca
    13501 gccacagcca cggttgccag caaagtaaaa ccacctgcgc caacagcaac agaaaataga
    13561 gcacatgcca ggtgcgagga aacgaagccc gccgaccgct caacaaaaac gccagcaccg
    13621 ccggaatacc gggaatcagc cccagccaga aattatcgtg atcgggataa aacagattta
    13681 gcagcgcagt accctgctcg cgcgacgcac cggcaatgac aaacagcacc caggttcgcg
    13741 cctgaagcaa tagcacaagc cagaagagca agggtaaacg caggcgtccg tgcgcatcat
    13801 aatggacagg atggaactca gtactcttca tcttcaatca aacgcttacc cagactcagc
    13861 acgtcggcgt gttcatatcc caggcgttca tacattccga gcaccatgtc gttatcttcc
    13921 ggcacgttga tctgaatttt cgggcagccg cgagcaatca gctttttctc cagccggtta
    13981 agcaacgcat tggcaattcc acgcccacga aactctggat gcacgccaag ataataagca
    14041 gacccgcgat gcccgtcata accgcccatc accgttccga ccacttcacc gtttacctca
    14101 gcgaccaaaa acaaactgac gtcatggttc atcttacgct cgatgtccat ttccggatcg
    14161 ttccacggac gcagcaagtc gcaacgctcc caaagggtga tgacctcttc gaaatcttcc
    14221 tggcgaaata cgcgtatctc catggtattc gttacctttt tgcgggttaa aaggctgatt
    14281 atggcgtgaa cggtcgaatt agccaatatc tgacgaaaat cggttgaaaa agtggcataa
    14341 tggggagttg tcaactattg aaatgaaaag taaaacaatt ctcaacagca aaccgtcgta
    14401 acggattacg cgatacgata taacatctgg aactttatta ttacaactca ggccgtatga
    14461 gcacttttaa accactaaaa acactcactt cgcgccgcca ggtgctgaaa gccggtttag
    14521 ctgccctgac gttgtcagga atgtcgcaag ccatcgccaa agacgaacct ttaaaaacca
    14581 gcaacggaca cagcaagcag aaagccaaaa aatctggcgg caaacgtgtc gttgttctcg
    14641 atccaggcca cggcggaatt gataccggag cgatcggacg caacggttcg aaagaaaaac
    14701 atgtggtgct ggcgattgct aaaaacgtcc gttccatttt gcgtaatcat gggattgatg
    14761 cgcgtttaac gcgttctggc gatacgttta tcccacttta cgatcgcgtt gaaatcgccc
    14821 ataaacatgg cgcagatctg tttatgtcaa ttcatgccga tggctttacc aacccgaaag
    14881 ctgccggtgc ttcggtattt gccctctcta accgtggggc aagtagcgca atggcgaaat
    14941 acctgtctga acgcgaaaac cgcgccgatg aagttgccgg taaaaaggcg actgacaagg
    15001 atcacctatt gcagcaagta ctgtttgatc tggtgcaaac cgatactatt aaaaacagcc
    15061 tgacgcttgg ctcgcatatt ctgaaaaaga ttaagccggt gcataaactg cacagccgca
    15121 acaccgaaca agccgcgttt gtggtgttga aatcgccgtc ggttccttcg gtgctggtgg
    15181 aaacctcgtt tatcaccaac ccggaagaag agcggctgtt aggcacggcg gcgtttcgtc
    15241 agaaaatcgc cacagcgatt gctgaaggcg tgatcaatta tttccactgg ttcgacaacc
    15301 agaaagcaca ttcgaaaaag cgataagtta tgaaacccga cgcacaccag gttaaacagt
    15361 ttctgctcaa ccttcaggat acgatttgtc agcagctgac cgccgtcgat ggcgcagaat
    15421 ttgtcgaaga tagttggcag cgcgaagctg gcggcggcgg gcgcagtcgg gtgttgcgta
    15481 atggtggtgt tttcgaacag gcgggcgtca acttttctca cgtccacggt gaggcgatgc
    15541 ctgcttccgc caccgctcat cgcccggaac ttgccgggcg cagtttcgag gcgatgggcg
    15601 tttcactggt agtgcatccg cataacccgt atgttcccac cagccacgcg aatgtgcgtt
    15661 tttttattgc cgaaaaaccg ggtgcagagc ccgtttggtg gtttggcggc ggcttcgatt
    15721 taaccccttt ctatggtttt gaagaagacg ccattcactg gcaccgcacc gcccgtgacc
    15781 tgtgcctgcc atttggtgaa gacgtttatc cccgttacaa aaagtggtgc gacgattact
    15841 tctacctcaa acatcgcaac gaacagcgcg gcattggcgg gctgttcttt gatgatctga
    15901 acacgccaga tttcgaccac tgttttgcct ttatgcaggc ggtaggcaaa ggctacaccg
    15961 acgcttattt accaattgta gagcgacgta aagcgatggc ctacggcgag cgcgagcgca
    16021 attttcagct ctaccgtcgc ggtcgttatg tcgagttcaa tctggtctgg gatcgcggca
    16081 cgctgtttgg cctgcaaact ggcgggcgca ccgagtcgat cctgatgtca atgccgccac
    16141 tggtacgctg ggagtatgat tatcagccaa aagatggcag cccagaagcg gcgttaagtg
    16201 agtttattaa ggtcagggat tgggtgtaac tccctcaccc ccattcccgc atccgctgat
    16261 gcaacgtcaa cgacggcttc tcggcaaaca gctgctggta atccgtggca aattgcccca
    16321 gatgccagaa tccccactgc atggcgccgt cttttaccgt tgtgctttgc gaccacgggc
    16381 ttatcagttc gcggcgtacg gcgttcaggc gaatgcgttt cagccacgcg tttgggccaa
    16441 tgcctaaaat agcgtgaaac gcgttttgta gcgtgcggcg actgacatgc agttgattac
    16501 acaaatccag caccgtcacc ggttcggaca tgttttccag cacatattca cgggcgcggg
    16561 aaagcaaccg acggtaactc tgatgactga tgctttccgc cgtcaccatt ggttgcgctt
    16621 cttccagcat cgcccccatc gccattagca aattatcccc cagcactttt cgcactgcag
    16681 gctgatggag attttccggg ttctcgcaaa acgtcgccag cgcctgttgg acaaagcccc
    16741 acagcgcggc tttatgctgc tctttcactt ccagcgccga ctggctacgc agcatatgta
    16801 atacccgatc cggattgtgc aaaaagttag cctgacgggt gatgacatct tcagacagca
    16861 ctacgcccag gatcgtataa tcgtccggcg tacttaattc aaactcggtt ccaccggggc
    16921 gcgtcgctat ttctgcgctt cccagacatt gcgaaccgat aaatccctgc tcaccgcgcg
    16981 tcgccggaat gccaaaccag aacgagttcg gccagaccag gcacgactga cgcagcgcca
    17041 gaccggtgta ttcacgaaaa acctgaatat catcgagtag aatttccgta aattcaccat
    17101 gaaacttgcc cggatgcagc tgatcgtaaa tctgctgcca ggcggtaatc gtcaaagcat
    17161 gttcatagac atccgttgtc tgtcgttgat aaacattatc cacttcgacc ttcggcgtga
    17221 gcttcaggtt ttcgggtaag ggttcatgat aaagatggtg caaattggct gtacgggtct
    17281 ttttcatgat gttaatgccg ggtgttgtag gacacccggc acctccgaca ggttaatggg
    17341 gcttgagacg ataacgacta ctgcgtttac gtaacgtccc ggcagaaaag agctgttcga
    17401 gcgcatttct ggctttttcc agcggccagc caaagtgggc ggccacttct cctgccgtca
    17461 ttccctgaca tactgatgtt aacagcgcca acaactcgtc agcagattcc gcgacgataa
    17521 ctggcgcgtc gggttccttt acgggttgct tcggctggcg gttaaagcca gtaaccagcc
    17581 actgggtgtc atcgtctggc cgaccgatct ccttacggct gatgacacgc ccggtacgca
    17641 tcgcggcagc acaacctgcg tccagcgccg cacgacacgc cgtcagatcg ccttccacca
    17701 ccagcgttaa gcgaccaggg tcaagcactt cgtgactgag cagacgaacg ttagctgctt
    17761 tgagcatggc atccgctgca tctatcgcgg cgaccatgcc gtccacttcc agcaatccca
    17821 gtgcattgat catcggcaac ctccgttacg cacgctggat tggattacgc gcgatttcca
    17881 gcactgcatc ggtaaaggcg ttacaggctg ctttacacgc ggcctggcta ccggttaaaa
    17941 atgctgccga gtagttggtt tcagacggtg gcgggacgta ggtcaccagc tgtacgtcgg
    18001 cagatttcaa cgcggcatca atgccgtagg tcgcttccag cggcggtgcc accagatacg
    18061 ccatcggatc gccaagcgtg atcccggagg ttgatgaaag ataagaaccg gtacgcgaaa
    18121 ccacatgcgc caggaaggcg gtgttttctg cgtcattggc ccactggaaa gctgcgccat
    18181 tttcaatatg tgcaaccatc gcatccagac cggcacgcac ttccgccggg tttggcccac
    18241 caagcataat cagcacttca ccggcagtcg gtgacgggcc gtgtgccgct cccgcataca
    18301 gcgagcgacc atataccact tcgaccatcg cttgtttggt cgcttcgtca gccgcaatgt
    18361 aggttacgtc gtcagaatcg gcagaaatca gcccgaggct acgaatatgc tgcggcaatt
    18421 ttagttcacg cgcaaattca gcgttaacgg aggcaatcac ccgcatggcg gttaccgacg
    18481 gtcgaatcaa atctaaagct ggcatgatgc ctccttatcg ggtcatgttg atgccggatg
    18541 ctttctgctc cagcatacgt ttcgccaaat ccacaatgac ggctgcggct tcaaccggcg
    18601 gcgtgccgcc ctggtgaatg ttagagatac aggtgcgatc ggcctcaacg gtggtcgcca
    18661 tacgcggcga gtaaacggcg tagcaggaga ggctttctga ctgccctaac cccggacgtt
    18721 cccccaccag caggatcacc actttcgcgc cgaggatctc accgatctga tcttcaatct
    18781 tcacgcgacc ataacgaaca aagaatggcg tgccgacttt cagcccggcc tgtttcaggc
    18841 ccgccatcag cggcggcagg atctcttcgt agttgacggt gatggcatca gttgacaggc
    18901 catcagaaat aacgacctgt acgtccggat tagcaacaca ctgtgctttc agcgcttcaa
    18961 cagcttctgc acacaggcgg cggcccatat ccgggcgagt caggtagaga tttttgtcgc
    19021 taatttcaga gcgtacttcc agcaagccct gcgctttcac ccactcttcc ggcacttctt
    19081 tcagtacggt atctttcgaa cgagagtgat cggccaggaa acgcagcagc gcctgagtac
    19141 gtggacgcgg accggcacga ccggtacaaa cgcgggccac ggtgctgcgg cgcagttctg
    19201 ttaatacgtc tgcgcgatgc gggttttcaa caccaatcca cgcttttgct tcagcggaac
    19261 ctaaatccag cgcacagctt tccgaggtca ccggtgccgc acagttggtg gtggcgcact
    19321 ttgcttctga cggcgcgggg gccgcttgtc ccattgacgc catcacgctg cgtacaattt
    19381 cttcaatctg tttttgatcc atgatgtgtt atctccgcgt catcagaaga acagtgacgg
    19441 atcgcccgcc cgtttggtca ggcgaccgtt tgccataatg cccatgcttt ccagccagcg
    19501 ttcaaactct ggtgacgggc gcaggttgag taactgacgc acagtggcgg tatcgtggaa
    19561 tgcggtggtc tggtagttga gcatgatgtc atcacccagc ggcatcccca tgatgtagtt
    19621 gcagcctgcg gtggcgagca ggatcatcag gttttcgttg aggttctggt cagagtcagc
    19681 gtggttggtg tagcagcagt cacagcccat agagatgccg ctcagcttgc ccataaagtg
    19741 atcttctaag ccagcacgga taatctggcg gtcgttgtag agatactccg gcccaataaa
    19801 gccgaccacg gtgttgacga taaacggatc gtaatgacgc gccagcccat agttacgtgc
    19861 ttccatcgtc acctggtcag cgccgaagtt agcgccagcg gatagcgcag agccttgtcc
    19921 ggtttcgaag tagaggcagt tttccccggc gatacgattg aactctgcgc ccactgcgcg
    19981 cgcttcgtcg agcatcgcca gttccacgcc aaactctttc agcccttttt cgctgccaca
    20041 gatactctgg aaaatcagtc cgccaggcgc gccgcgacga atcgcttcga tctgggtggt
    20101 gacgtgcgcc agtacgcagc cctgagttgg gatgttgaat ttgtcgatca cgccataaat
    20161 ggtatccagc acgcggctta agttttccac gtcgtcagtt accgggttaa cgccgatcac
    20221 cgcatcgccc accccgaagg aaagcccttc atagatttgc gcagcgatac tttgcacgtc
    20281 gtcacgggta tcgttcggct gcaaacgggc gctaaaggtg cccggaatac cgatggtggt
    20341 attggccttt ttgattaccg gcattttctt cgcgccgtag atcaggtccg cgttggagca
    20401 aatcttcgct accgccgcga ccacttccga ggtcagccct ttgcgggtaa aggcaatgtc
    20461 gtccacgctg gtttcatcgc tcagcacata ctcacgcagt tcgctgatgc tccagttttt
    20521 aatctggttg taggccgttt cgttaacgtc gtcctgaatc agccgcgtca cgcagtcatc
    20581 ttcataggca atcaccggat tattgcggat gtccgctacg gtcatttccg acaacacctg
    20641 ctttgccgcc acgcgctcct gtgagcttgc cgctgcaacg cccgccagca catcccccga
    20701 acgcagttcg ttggctttag ccagcacctc ttttacatcc ttaaactgat atacattgcc
    20761 gaacaatgtg gtctttagtt tcataagtcg ttccctcagg aagaagtgat gagtaacatt
    20821 tcttccagca ttcgaacaat tctatttcat cgttagccct tgtcctgtaa ggtgttaccc
    20881 ttaatacgcc cgttacttat cctgccggac acaaccgaac gcgccggata catgagcggg
    20941 gttatcgagt cgttagatcg aggtatccgg tttgagcaag acaccactga cagcaaaggc
    21001 catagaggcc gcccagccgc aggacaagcc ctacaaactc actgattcaa tgacgcctgg
    21061 ccttttcttg ctggtacatc ccaacggcag caaatactgg cgattccggt attggataga
    21121 caagaaggag cggttacagg ctattggggt gtatccgctg attagcctca aggaagcccg
    21181 caaacgcgcc acagagagcc gtttactgat agcccaggga attgacccaa tggatttggc
    21241 acgcaaggag aaagccattg atgctcttaa tgctatcgcc agttttaaag ctgtagcaga
    21301 agactggatg gctaccaagg tgagcggatg gagtgagagc tatacaaagc aggtcagatc
    21361 ggcactagag aaagatgttt accccgtgct aggtaagcgg tcgatagtgg atataacggc
    21421 ccgtgacgtg ctggcgttac tacagaaaaa ggagcgcacc gcaccggaac aagccaggaa
    21481 attacgccag cgtatcgggg agatcttcaa atttgccgtt atcactgaac tggttaaccg
    21541 gaatccggtt gcagatctgg atacggcact gaaagccaga cgcccaggcc ataacgcgtg
    21601 gataccgatt aatgaaattc cggccttcta caaagccctt gagagagctg ggagcgttca
    21661 gattcagacg gcaatacttt tgctcatcct cacagctttg aggacggcag aattgcgcct
    21721 gatgcgctgg gaatgggtag atctgcaatc ggcaacaatc acattgcccg ctgaagtcat
    21781 gaaggcccgc cgaccgcatg ttgtcccgct atcccggcag gcggtcgagc tattacagga
    21841 ccagtttacc cgcagcggat acagtgcttt cgtctttcct ggccgattca tggacaagcc
    21901 actttcagcc agtgccatcc ttaaagccct ggagcgtatc ggatacaagt cgatagccac
    21961 aggccacggc tggcggacca ccttttcaac ggcgcttaat gagtccggca ggtactcacc
    22021 agactggatc gaaatccaac tggcccacgt tccgaaaggc gtgcgcggtg tttataacca
    22081 ggcggcctac ctgaagcaac gacgggccat gatgcaggat tatagcgact gtattgattc
    22141 aatactggct ggtgacggca atccgcttga gccggaatga ataattaagc gggtttccga
    22201 cctactccac cggagcagac tagataaata ctggacccct tcagaataaa tattcacctg
    22261 ccggattcgt ccggcttttg tttataaatc atccaatcgt gaaattatta gaattaaact
    22321 tacatactag tttactagcc agattaggct actagtatat aacatcaata aaatcatgag
    22381 gttagtcaaa agttgccgtc aggggtcatg cgggcgtaac attagatcaa cgaaataaaa
    22441 aagcccggaa aggcggcaac cagttccggg cggtatgaga aacaagctaa aggagaattt
    22501 ctcatgtctg atatgctagc agtagaacaa gaaacaaaca atgatgttcg ccaatattta
    22561 aacaaaatta acgagttacg gaataaagca cccaaaaacg aagagactaa acacgaagaa
    22621 cacacgccag atcattacga ggcaaaacag caggagcaag cctggcgtgg caacctgcgc
    22681 tacctggaca cattaaaccg acttgatgag gtattgcctc gcaaactgta cgaaaggtgg
    22741 gagaaagaac atacagtcaa cgacgaggcc gtgctacgcg ccctttgcta ctttgctggt
    22801 actggcaaaa actcacagtt gggatggtgc agggttggca ggggaacaat cgacaagcga
    22861 gccaggctat caaaaaacac ggttaaaaaa tgcctcgatc gtctggtcca ccacttcaaa
    22921 ctggttgagc gtactgaagg ctatataccc ggatcggcag aaagggaatg caacgagtac
    22981 cagctattat tcaagccgta caacatgaaa taacggaatc aggcgggcaa tgttcccgcc
    23041 ttttctttgc cttaattcca tgaattcatg ggtgggtcaa aggttgccgt tagggtaggt
    23101 caatttgtgc ctttaggtgg gtcaaaagct gccgttaagg tgggtcaaaa gttgccgagt
    23161 aagagtttca ctattaagaa ttctaggcga cgggagtttt ttagcctccc ggtcggtcac
    23221 agagagtcgc ctaggattaa aacagaagaa tttattgccg cgcagcgctg gatgaatgca
    23281 atcatcaata ccgctttggc gcgtcatcct tcccagcaag ctggaaaggc ccattcactc
    23341 acgcacaacc tgcaaaccag accaacagac catcatggcg attcgttcat gggtagcgcg
    23401 ggtatatctc aaagggtgat ttcttcttgg tggaccttcg gaagacttcg agcaattgcc
    23461 agcttaaaga cagcttagca gtgatggtta tcatcaacag ctttctgagc taccgcacag
    23521 acacacagca gcagtaagca aaccgaaggg aatgcaaagc aaaaggattc gccacactgc
    23581 aagaccaatg gtttttgtca cgtatacaga ccaggcaagc aacagcagca aagcagcgta
    23641 tagccaggtg cagatagtac catcacacaa cgcacagcag gctgaggtga acactcaagc
    23701 aactttatcc ccgccaccga ccaccgcaaa ctatcctgac cgtgccaaat tcgaacgtaa
    23761 tgccacctgg aacgcgatgg agacacataa gggctacgct tgcttagctg aaaacttcac
    23821 agcgtcacag cgctatttag acgcattaga gtcgatgcga tttcattaaa ttttttagcc
    23881 atccagaaat cggcctgatt ctgagccgat tgccagccgg actactgacc agatcggacc
    23941 aatcatccaa ggttggatga atgaaatcac acgcagatcc tgccaggcta tttcatccca
    24001 ccacacagaa cgctgagatc tgatattacc gccgcaccat tccacgccag acgaatcaaa
    24061 acaggcttac ccttccaagt ggatagctaa aaattaaggc tgtcagaatg cgttatattg
    24121 cgtttaaacg gcatcgaatt ttaatgtgcg caatggtgcc agattgcagg caaaaaaaat
    24181 cccgccggag aaagcgggga agtgaaatga atcgttgtag ttatagatca cattttgagt
    24241 aagggtaacc agcccttgag taatattatc cctgcattac gtgagatgcg caattatttt
    24301 ttgctaatta aaagttttgt aaacattaca gaatatcccc tttcactata tggtaaaaga
    24361 tgtgaaacag ggacaggagg aatataatat taagtatcaa cagtaatgtt ggcctttaat
    24421 ttcattcagg gcgcggtgag ggttttctcc atattttcct caccgctact ttttgaggtg
    24481 agaacatgaa tgatagcgaa ttaatgacgg ttaacgaggt agcagcttta ttcggcgtta
    24541 cccgtcggac aatattccgg tggatgaaca agattaaagg ctggcccgtc ccggtcagcc
    24601 cgattgactc aagaattaag tttatccgat ctgagatttt ggaattctat aaaaacaaag
    24661 gcgctcgcca ccaatgaaaa cagctactga gattatcaaa taacctattg atttaaaaag
    24721 ctagctaccg caatttctga aggaataggc agcatgaaaa tcaaagcaaa cacaattaaa
    24781 gaaataacat caatcaaagt aaaaggagca gatgccatgc attcattaga cagttatttt
    24841 cagcgcacga cagcccctaa atcagccgcc caggaacgcc gtgaggaatt ccaggaaaaa
    24901 gtgatgcgtt ctgctgatta tattgcggac aagttcgtgg aaacggttcg gccacttgtt
    24961 gatgaagtag ctgataaact tcagtctgaa atgccggaag acatggaagg cactgcaaag
    25021 gcgcgactcc tttttgaatt aagtcgtcgt ttcggcgtgt caataagcac ttttaaataa
    25081 tcggaggagg aaagacaatg gacgcatttg atgtactgac aaacaaaggc gatgaactgg
    25141 tagcacagcg tgataaaacc gccaacgaag gggaacgcgc actgttaaat aagcagatta
    25201 aagcgatccg tatggcccag tttaaattaa tcagcaatga agttatcgaa ataattaaac
    25261 cgcagatccc acaaatcgtt gctgacgcta ttaaagccgc cggactggag aaacgcattg
    25321 ccgggctgaa aacgaacgta gagaaaatgg agatgcacaa acagagcggt gctccgactc
    25381 cggatgagta cttctcaccg gatgctgagt ttatggcgca ggttgaggaa cgtctgggta
    25441 gctgtctcac cgaggaacag cgtcgctatt ttgatggagt ggatagttcg gcaggtattg
    25501 acctgaactc gtatttcggt cgtgaaattg agcattttga tgccgccccg tctatgccgg
    25561 agccagaacc agaaatggcc gccgatcctg aagctgagca acggaaacgc gtattcaatg
    25621 cgatagggat tcggtactga tcggataaaa gaaagccggg aattaccccg gctgacttaa
    25681 caatgtgacc aatgaacaaa tcggacaaga caaacaggat aagcagtctc gaaacaatgt
    25741 tcgggaacta tattagcaca agtccggaga aaatcatcat gttggcacta cgcatcatgc
    25801 agggcatcgc taagaccctc gcagaacatg tcttagacct gaaacattcg ccactgagca
    25861 aacaggccat gaagcgccaa acgcttcgtc tgtgggctga atattcgctt ggaactatca
    25921 ataaaatcat cgacatgaaa tcggggccga gcaaccagag cgcggaggaa atggagttca
    25981 tcaggcgttt aattctcatt cgcagagaca ttcatagcca gttacacagc gtgggaatcg
    26041 atattaatga tgggacgggc gattaatttt cggacaagta ctatgcgcgc gcgaggagct
    26101 gaacatgaag caagatattg cagacaggct ggagatactc gaaggccagc gggcagaagc
    26161 gaaacaacta cgtaagcagg ccagaagagc gcacaggaac aacgaagcag agttactaac
    26221 caaatacatc agtttcacca attactgcat ctacgagtgt tacaaggaag atgcagagga
    26281 ctggctggat tcattgccag aacagtactg agcagaataa caccacgtca tgttgttctg
    26341 aggtgagcat atcacctctg ttcaggtggc caaatttagt gtgccacttc agcatcaccg
    26401 ttgaaatcac aataacgatt tttttgtaaa tatgctctca catatgtagg aatagaccag
    26461 gttggcattg aaacacatct accatgacca taataaagta attttttgat atgtttatgg
    26521 ttttatatta attaaaatat atctaaaatt gatagtttta aaattgcaaa atccagattg
    26581 tgtttgtaat gtttaatctg attgtattga gtggcgttta ttatgaaaaa taaatcaggt
    26641 aattcttgtt atagagaagc agtcgatatt catagctcac ttgagctttc atttatattg
    26701 cataaatatc atttgcgtgt agatgagaag aaatctcgaa atgtattcag cgccatacgt
    26761 gatgagctca tccttgatgg ggattcacga caaaacttag ctactttctg tcaaacatgg
    26821 cttgatgatg aaatccgtga gttgcttaat ttagctattg ataaaaacat gatttataaa
    26881 gatgaatatc cacaaatggc ggaacctgaa ggtcgctgtg tgcgtatgct ttccgattcg
    26941 tggagtttcc ccgattcgcg acacacctta ggttgctcaa ccattggttc atctgaagct
    27001 gctatgctcg gccgcttggc gttaaaatgg cagtggtgta aaaaacgcga agtacaaggt
    27061 aaatcaaccg aacctgattt gcgatcctgt gcaaatatgc tggcataaat tctgccgtta
    27121 ctgtgatgtg aaattgtgtg aaattccact tgatgatcat cacctgataa tgaatgctaa
    27181 agaggttttg aagcatgtcg atgaaaaaac tattggtgtc gttacgactt taggtattac
    27241 ttatttttgt aaagcagatt gcgttagtgt ttttaagata agtgttaaag ttaatattag
    27301 tttttatatt tacatcgtaa ttatatgttt ctattttaaa aatattcgag agtataaaaa
    27361 tgtc
//
LOCUS       DBKEKI010000049        27237 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.049, whole genome shotgun sequence.
ACCESSION   DBKEKI010000049 DBKEKI010000000
VERSION     DBKEKI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.049"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..505
                     /locus_tag="ACUQPX_002012"
     CDS             <1..505
                     /locus_tag="ACUQPX_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HHV9056731.1"
                     /translation="FTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHD
                     FLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEY
                     VMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRPINLRCE
                     LLIPIRR"
     gene            complement(502..1149)
                     /gene="gpmB"
                     /locus_tag="ACUQPX_002013"
     CDS             complement(502..1149)
                     /gene="gpmB"
                     /locus_tag="ACUQPX_002013"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HHV9056732.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            1201..1722
                     /gene="yjjX"
                     /locus_tag="ACUQPX_002014"
     CDS             1201..1722
                     /gene="yjjX"
                     /locus_tag="ACUQPX_002014"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HHV9056733.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(1807..2133)
                     /gene="trpR"
                     /locus_tag="ACUQPX_002015"
     CDS             complement(1807..2133)
                     /gene="trpR"
                     /locus_tag="ACUQPX_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HHV9056734.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(2223..4160)
                     /gene="sltY"
                     /locus_tag="ACUQPX_002016"
     CDS             complement(2223..4160)
                     /gene="sltY"
                     /locus_tag="ACUQPX_002016"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HHV9056735.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDGIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGHSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            4371..6038
                     /gene="ettA"
                     /locus_tag="ACUQPX_002017"
     CDS             4371..6038
                     /gene="ettA"
                     /locus_tag="ACUQPX_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HHV9056736.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(6145..6312)
                     /gene="nadS"
                     /locus_tag="ACUQPX_002018"
     CDS             complement(6145..6312)
                     /gene="nadS"
                     /locus_tag="ACUQPX_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HHV9056737.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(6346..7578)
                     /gene="nadR"
                     /locus_tag="ACUQPX_002019"
     CDS             complement(6346..7578)
                     /gene="nadR"
                     /locus_tag="ACUQPX_002019"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HHV9056738.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     IRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(7599..8981)
                     /gene="radA"
                     /locus_tag="ACUQPX_002020"
     CDS             complement(7599..8981)
                     /gene="radA"
                     /locus_tag="ACUQPX_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HHV9056739.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(9030..9998)
                     /gene="serB"
                     /locus_tag="ACUQPX_002021"
     CDS             complement(9030..9998)
                     /gene="serB"
                     /locus_tag="ACUQPX_002021"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HHV9056740.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            10104..10748
                     /locus_tag="ACUQPX_002022"
     CDS             10104..10748
                     /locus_tag="ACUQPX_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HHV9056741.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            10776..11792
                     /gene="lplA"
                     /locus_tag="ACUQPX_002023"
                     /pseudo
     CDS             10776..11792
                     /gene="lplA"
                     /locus_tag="ACUQPX_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            11824..12087
                     /locus_tag="ACUQPX_002024"
     CDS             11824..12087
                     /locus_tag="ACUQPX_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9056742.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(12248..12967)
                     /gene="deoD"
                     /locus_tag="ACUQPX_002025"
     CDS             complement(12248..12967)
                     /gene="deoD"
                     /locus_tag="ACUQPX_002025"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HHV9056743.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLDDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(13024..14247)
                     /gene="deoB"
                     /locus_tag="ACUQPX_002026"
     CDS             complement(13024..14247)
                     /gene="deoB"
                     /locus_tag="ACUQPX_002026"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HHV9056744.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWNGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(14299..15621)
                     /gene="deoA"
                     /locus_tag="ACUQPX_002027"
     CDS             complement(14299..15621)
                     /gene="deoA"
                     /locus_tag="ACUQPX_002027"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HHV9056745.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGCGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(15748..16527)
                     /gene="deoC"
                     /locus_tag="ACUQPX_002028"
     CDS             complement(15748..16527)
                     /gene="deoC"
                     /locus_tag="ACUQPX_002028"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HHV9056746.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTNEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            16785..18321
                     /locus_tag="ACUQPX_002029"
                     /pseudo
     CDS             16785..18321
                     /locus_tag="ACUQPX_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
     gene            18293..19156
                     /locus_tag="ACUQPX_002030"
     CDS             18293..19156
                     /locus_tag="ACUQPX_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HHV9056747.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNGCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKICPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(19370..20152)
                     /locus_tag="ACUQPX_002031"
     CDS             complement(19370..20152)
                     /locus_tag="ACUQPX_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HHV9056748.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(20149..21222)
                     /locus_tag="ACUQPX_002032"
     CDS             complement(20149..21222)
                     /locus_tag="ACUQPX_002032"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HHV9056749.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(21344..21505)
                     /locus_tag="ACUQPX_002033"
     CDS             complement(21344..21505)
                     /locus_tag="ACUQPX_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HHV9056750.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(21632..22237)
                     /gene="osmY"
                     /locus_tag="ACUQPX_002034"
     CDS             complement(21632..22237)
                     /gene="osmY"
                     /locus_tag="ACUQPX_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HHV9056751.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(22630..24219)
                     /gene="prfC"
                     /locus_tag="ACUQPX_002035"
     CDS             complement(22630..24219)
                     /gene="prfC"
                     /locus_tag="ACUQPX_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HHV9056752.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(24310..24987)
                     /gene="yjjG"
                     /locus_tag="ACUQPX_002036"
     CDS             complement(24310..24987)
                     /gene="yjjG"
                     /locus_tag="ACUQPX_002036"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HHV9056753.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(25002..25448)
                     /gene="rimI"
                     /locus_tag="ACUQPX_002037"
     CDS             complement(25002..25448)
                     /gene="rimI"
                     /locus_tag="ACUQPX_002037"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HHV9056754.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(25417..25830)
                     /gene="holD"
                     /locus_tag="ACUQPX_002038"
     CDS             complement(25417..25830)
                     /gene="holD"
                     /locus_tag="ACUQPX_002038"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HHV9056755.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            25933..26964
                     /gene="rsmC"
                     /locus_tag="ACUQPX_002039"
     CDS             25933..26964
                     /gene="rsmC"
                     /locus_tag="ACUQPX_002039"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HHV9056756.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT         6300 a         7527 c         7075 g         6335 t
ORIGIN      
        1 attcacaatg ccagagcaca aatttgtcac cctggaagat acgccgctga ttggtgttac
       61 ccagagctac tcctgttcgc tggagcaaat ctctgatttc cgccatgaaa tgcgttatca
      121 gttctggcac gattttctcg gcaacgcgcc gaccattccg ccggtgctct acggcctgaa
      181 tgaaacgcgt ccgagtcagg ataaagacga cgaacaagag gtattctata ccaccgcgtt
      241 agcccaggat caggcagatg gctatgtact gacggggcat ccggtgatgc tgcagggcgg
      301 cgaatatgtg atgtttacct atgaaggtct gggaaccggc gtgcaggagt ttatcctgac
      361 ggtatacgga acgtgcatgc caatgctcaa tctgacgcgc cgtaaaggtc aggatattga
      421 gcgatactac ccggcagaag acgccaaagc gggagatcgc ccaattaatc tgcgctgtga
      481 actgctgatt cctatccgcc gttaacgctg cagctcatct aatgcagggg cgtccagatg
      541 cgagatgtcc cctgcagttt caacgaccca gccagacgcc agccacaggc tttcctgata
      601 atccacgcgc gaaatagaac agttacgcag acgtaagcgg cgctctgccc atgctggtaa
      661 tccgagaatc gtactcacca gacatcccag tgcaatacca tggctcacca gcagcggtcg
      721 gcttccctgc ggtaagtccc ggcaagattc cagtgccgca ttgacgcgat cgctgagctc
      781 ctgcattgac tcgccttcag gaatacgccc gtcaacggtg ccattgacca gctgccgacg
      841 ccagttctct tcttcttcgg tcagagaatc gatatgtctt ttttccagca cacccatgtt
      901 taattcacgc aggcgagaat caaagatgat gtcacagccg caggcctggg cgatgatttc
      961 cgccgtacgc cgggtgcgtc ctaaatcgct actgataata tgcgtaatgc caagctcttt
     1021 ggcacgggtt gccacctgca tcgcctgttg ctcacctttg gcggtcagcg ggctgtcaga
     1081 ctggccctga atacgtcgct cggcgttcca ctgcgtttca ccgtggcgga ctaggtatac
     1141 ctgtaacatg ctttttttcc gtatactgct gtaacgttaa ttttctgagt aatgctgatt
     1201 atgcaccaag ttgtctgtgc gaccaccaat cccgctaaaa ttcaggccat tctgcaggca
     1261 tttcacgaga tcttcggcga aggatcctgc catattgcat ccgttgccgt cgagagcggt
     1321 gtaccggaac aaccctttgg cagtgaggaa acgcgcgctg gcgcacgaaa tcgggtagca
     1381 aatgcccgcc gtttacttcc agaggctgat ttttgggtgg cgattgaagc tggcattgat
     1441 ggcgacagca ctttcagttg ggtggtgatt gaaaacgcca gccagcgcgg cgaggcgcgt
     1501 tcggcaactt tgccgttacc tgcggtgatt ctggaaaaag ttcgtgaagg cgaggcgctt
     1561 ggtcccgtga tgtcgcgtta taccggtatt gatgagattg gtcgtaagga aggggcgatc
     1621 ggcgtgttta ccgccgggaa actcactcgc gccagcgtct atcaccaggc ggtgatcctg
     1681 gcgctgagtc cgtttcacaa tgccgtgtat caggcattgt aggacggata aggcgttcac
     1741 gccgcatccg acattcggtg cacgatgcct gatgcgacgc tgccgcgtct tatcaggcct
     1801 acaaaatcaa tcgcttttca gcaacacctc ttccagccac tggcgcagct ccacgggcgc
     1861 ggctttcagg ctgttagatc cacgcgtaat cgtcgcgatg cccgcgccga gttcattttt
     1921 taactcacgc tgactcattt cgccacgcaa cagctcttcg acaatacgca cgcgagtccc
     1981 caacgcttcg cgctcatctg gcgtcagcat caggtttaac aacggtaaat ggagatcgtt
     2041 ttggtaggca ttcttaagca ggtcgacaaa acgtaaccac tcctggtgac gctgttctgc
     2101 catcgctgct gaatagggtg attgttgggc cataatatgt cgccattgtt agcgggggaa
     2161 gcaaaatgac tcccccggtt gtactcgcta aagagtacga tagcatatca taaacgtgcg
     2221 gatcagtaac gacgtcccca ttccgtggcg ctcatcaacg tcggtttatc ccccatgaaa
     2281 tagcggtagt aagcatcata agccagcacg ttcttcacat aaccgcgcgt ctcggagaac
     2341 ggaatactct cgacaaatgc cacagcgtcg atacgcccgg cgctgtggcc aagccaggtt
     2401 cgcacccgtc caggtccggc gttataagct gctgaggaga aaatacggtt attgccaaac
     2461 tgctgataaa catattgcag gtaactggtg ccaatgttga tgttcgtttc cggatccagc
     2521 aattgcccag ggctgctata accaggaata gagaacatct tcaccgtatg ggtcgctgta
     2581 ccaggcataa tctgcatcag gccgctggcc cctaccggtg atttcacttt cggattccag
     2641 gcgctctcct gacgggcaat cgccatcgca tagctttgcg ggatctcttt cccactggtg
     2701 taacgtttga aaagatcgtt gtaagccagc gggaatcgct cttccagatg atcccaaagc
     2761 ttcccggcga tcgttgcctg aacgctaaga tcccaccatt ggttattgaa agcataccga
     2821 gccagttgag cctgctctgt ttttgacttg ctcttcacca gattggccca ctcgctacgc
     2881 gcggtattat cgagattcca gtacatcaac tcgcgcacgc gcgccatctc cggcccctga
     2941 gtcagggtgc tgtcaacatt ctgcggcgct ttatcaatct tcagctcata ctcttcaccg
     3001 atgcgttgtg ctgcaaccat cgggtagaaa ccacgctgtt gcatgagttg atgcaaaatc
     3061 tctttcgctt cagcttcacg tccgcgttcc agcaataaat ccgcctgcca gtaacgccat
     3121 tcatctttct cttttgcttc catcggcaga cgcgccagcc aggtattcag gccgcgacga
     3181 tcgccggtgc caagcgccat tcgcacacgg cgttcaatca acgacgtgga ttgcgagcgc
     3241 ataatgccgt catcgcgcca tttcgcctgc tcatcggtga catcattgcc catcaaacgc
     3301 caggcgacga tatcgcgcag ctcctgaatc tgatcttcat taagctgctg cgcctgggca
     3361 agcgacggga tcatcagccg tgcattttcg gcatcctgcc gcgccacact ggcaaacgcc
     3421 accgccgcca tttgacgggt aaaatcggtc gcgccggttg tacgcgcgaa ggtcagtacc
     3481 gtattagggt tgttcgccag tgaaatgatt gccgaggcga tagtctggta atcggcaggc
     3541 atctgccctg ccagcactgt tactaggcct gtgttacccg ctttcatcgc cagacggata
     3601 cgctctaaat acgccagcgg atcttgttta cctgacgcac gccagacgct aaataacttg
     3661 tcacaggcgt taggctggct cttgccggtt agccacagct ctttcgcccc ttgccaggct
     3721 tcttcactct gcccggtgtt ccatttcgca tagtagtaat tacattgcgc ttcggtagtt
     3781 ccgggctttt ccgggctaaa ggctaacaag ccacgccagt cttcacgccg cgccagttca
     3841 ttgacgaaac gagattgcag cgtgcgagcg ggaggaagcg tggggttagc gcgaacaaag
     3901 ttagtgaccg tcaccgccgg ttgattcatc aggtcatcgg tgatctggcg gtattccagg
     3961 tagggataaa gcggataatc cttcagtcca ggcatcattt gttcgaccac atccatctgt
     4021 cgattatccc aggcctgctt gatttgcgcg taacggctac gctgctcatc cagtgagtcg
     4081 gctcgcgcca cgctgctgac cgtcagcaga cagacaccgg cagccaacag ccgccaggta
     4141 acttgtttgg ctttttccac aagcgcatcc tctaagtgta aatacatcaa tgcagcgtca
     4201 tgccgcgtaa tagatttatg ctaaccagtc attgccgttt acgccacgtt acggacactt
     4261 ttttactttt actgcgaagg tgatcgacca actgggatta ggcggggaaa caggctacac
     4321 ttcgcctttg aaaactcatc atcacgataa acatagaggc gaagtccaac gtggctcaat
     4381 tcgtttatac catgcatcgt gtcggcaaag ttgttccgcc gaaacgtcat attttgaaaa
     4441 acatctctct gagtttcttc cctggggcaa aaattggtgt cctgggtctg aacggcgcgg
     4501 gtaagtccac cctgctgcgc attatggcgg gcattgataa agacatcgaa ggtgaagcgc
     4561 gtccgcagcc agacatcaag attggttatc tgccgcagga accgcagctg aacccggaac
     4621 acaccgtgcg tgagtccatt gaagaagcgg tttcagaagt ggttaacgcc ctgaaacgcc
     4681 tggatgaagt gtatgcgctg tacgccgatc cggatgccga ttttgacaag ctggccgctg
     4741 aacaaggccg tctggaagag atcattcagg ctcacgacgg tcataacctg aacgtacagc
     4801 tggagcgtgc ggcggatgcg ctgcgtctgc cagattggga cgcgaaaatc gctaacctct
     4861 ccggtggtga gcgtcgtcgc gtggcgttgt gccgcctgct gctggaaaaa ccagacatgc
     4921 tgctgctcga cgaaccgacc aaccacctgg atgccgaatc cgtggcgtgg ctggaacgct
     4981 tcctgcacga cttcgaaggc accgtggtgg cgattactca cgaccgttac ttcctcgata
     5041 acgttgcagg ctggatcctc gaactggacc gcggtgaagg tattccgtgg gaaggtaact
     5101 actcctcctg gctggagcag aaagatcagc gtctggcgca ggaagcttca caagaagcgg
     5161 cgcgtcgtaa gtcgatcgag aaagagctgg agtgggtacg tcagggaact aaaggccgcc
     5221 agtcgaaagg taaagcacgt ctggcacgct ttgaagagct gaacagcacc gaataccaga
     5281 aacgtaacga aaccaacgaa ctgtttattc cacctggacc gcgtctgggc gataaagtgc
     5341 tggaagtcag caacctgcgt aaatcctacg gcgatcgcct gctgattgat gacctgagct
     5401 tctcgatccc gaaaggggcg atcgtcggga tcatcggtcc gaacggcgcg ggtaaatcga
     5461 ccctgttccg tatgatctcc ggtcaggaac agccggacag cggcaccatc actttgggtg
     5521 aaacggtgaa actggcatcg gttgatcagt tccgtgactc aatggataac agcaaaaccg
     5581 tttgggaaga agtttccggc gggctggata ttatgaagat cggcaacacc gagatgccaa
     5641 gccgcgccta cgttggccgc tttaacttta aaggggttga tcagggtaaa cgtgttggcg
     5701 aactttccgg tggtgagcgc ggtcgtctgc atctggcgaa gctgctgcag gttggcggca
     5761 acatgctgct gctcgacgaa ccaaccaacg acctggatat cgaaaccctg cgcgcgctgg
     5821 aaaacgccct gctggagttc ccgggctgtg cgatggttat ctcgcacgac cgttggttcc
     5881 tcgaccgtat cgccacgcac atcctggact accaggatga aggtaaagtt gagttcttcg
     5941 aaggtaactt tactgagtac gaagagtaca aaaaacgcac gctgggcgca gacgcgctgg
     6001 agccgaagcg tatcaagtac aagcgtattg cgaagtaatg cgtaaaatgc cggatgcggc
     6061 gcgaacgcct tatccggcct acaaaaccag caatttcaat acgttgtaaa actgtaggcc
     6121 tgataagacg cggtaagcgt cgcatcaggc attgagcacc gaatgccgga tgcggcgtga
     6181 acaccttttc cggtctacaa cagccttagc atcctcaatt tcgtggcggg tcacccgtgc
     6241 aggttttttc aaaccttgtt taatctcgac agcctcttcc agagaggttt tcaactcatc
     6301 aaaaaaactc attttatacc tcgcctttga gcagtttcgt cacggttatc cttgctcccc
     6361 catcatctcc cgcaccagct ccacgcagcg caggaaacgg ctgtcgtaat cgtcctcttc
     6421 aacccgcacg aattcgatat tgttctcttc cagcatctcc accagcaagt tctggaactc
     6481 tttgcgatcc accgaactgc cgaggctgcg taaaccatcc gccacccacg gcgtgttgtt
     6541 ctccagcagg atcaccagat cgaaacggta ttcatcaatc agcgcctgca cgaacggatg
     6601 ctcacgccct tcgtactttt tgcagaacgc ctgggtagtg acaaaatcgg tgtcaataaa
     6661 cgccacttta ttggcgtatt tcactgcaaa atcaatgtat tgtgcgtggc ccaacgcgat
     6721 tttatcgtag tcggaatact gcaatgcgat ctcatcaccg ccgaggtgtg aaaagacata
     6781 atcgcggcca tattcccacg cactggtggt gttgaagata ttggcaagtt tgtttaccag
     6841 ggtggattta ccgctcgact cgccgccaag gatcgccacg gtacgcacaa aaaacggctt
     6901 cacttcggta ggaatatatt cccagtagcg gaacgggttt tcgcggatct gcgcaccgct
     6961 gatactcata aaggtacgtt tcggatcgac cagcaccgtc tcgatcccca gatgttccat
     7021 atactgtggc gcatcggctt cttccgaggt gtagatcagg tccggctgga tccccttttc
     7081 agccataaac tttttgatgc cgttgctcca cacatcccag ccatgcggat acggctccat
     7141 gccctcttcg ttgaaagcat gaatacggat atttttctga tatttaaagg tttgcaatag
     7201 ccagcgcaga cgatccggca ccgtgggctg ctgcgacatg gcgctgtctt caaacaacgc
     7261 gcggtcgcgg atatcgtcaa aacccataat gatatgcagc tcgtcaacct ggctacaggc
     7321 gcgctggata aggtagatat gtccggtatg cagcgggtag aacttaccga atacgacacc
     7381 aatggttttc ttctgccggg gaaattcaag ccccaaaaaa cggtgcaatg cctccagctt
     7441 ttgcgcgctg gggcttttaa ttttggcatt cagtaactgg cttaaatacc ctttggtcat
     7501 accgctggca tcagctacct gctgtagcgt gcagccctgt tgcttgatgg cagttttcag
     7561 gtaatcaaat gacgacatat ctccctccgt atctctcatt ataagtcgtc gaacacgcta
     7621 agcgcgtcgg agagtttttt aacgccaaaa atctgcatcc cttccggcgc ttttttcggt
     7681 acgttagccg ccggaacaat cgcccggcga aaaccgtgtt tcgccgcttc agaaatccgc
     7741 tcctggccac tgggcaccgg gcggatctcc cctgccagcc cgacttcacc aaacaccacc
     7801 agatcctgtg gcagcggtct gtcacgcagg ctggaaacca tcgccagcag taacgctaag
     7861 tcggcgctgg tttcggttac cttcacgccg ccgaccacgt tcacaaacac atcctgatcg
     7921 gccatttgca gaccaccgtg acggtgcaag acagccagca ggattgccag acggttttgt
     7981 tccagcccca ctgccacgcg gcgcggattc gccattatcg agtgatcgac cagcgcctga
     8041 atctccacca gcaacggacg cgttccttcc cacaccacca tcacggagct gccggaggtc
     8101 acttcatctc cgcgacttaa gaaaattgcc gaagggttgc tgacttcacg cagcccctgt
     8161 tcggtcatcg cgaagacgcc cagctcattc accgcgccga agcggttttt atggctgcgt
     8221 aaggtgcgaa aacgggagtc ggcatcgcca tccagaagca ccgaacagtc gatacagtgt
     8281 tccagcactt tcgggccagc cagcgaacca tctttggtta cgtgtccaac catgacaatc
     8341 gccacaccgc gcgttttggc gaaacgcgtc agataagccg ccgtttcacg cacctgcgcc
     8401 acgctaccag gcgatgattg tacatccgcc atatgcatca cctggatcga gtcaattacc
     8461 atcagcttcg gttgctcttc ttcggcaatc aggcagatct gctcgatgct ggtttccgac
     8521 aacatattga gattgtcagt cggcaggcca aggcgatgag cgcgcattgc cacctgttgc
     8581 agcgactctt cgccggtgac atacagcgtt ttcatctgct gggccagttt gcacagcgtt
     8641 tgcagcaaca gcgtggattt ccccgcacca gggttaccgc caatcagaat ggcacttcct
     8701 ggcaccacgc cgccgcctag tacgcggtcg aactctttaa atccggtgga aaaacgcggc
     8761 agctcttcaa ggctgatatc ggagagtttc tggacttttg ccaccccggc gctaccggca
     8821 tagccgctga gacgctcgtt acgcgccacc gttggcgacg cagcaagacg cacctcggtg
     8881 atggtgttcc aggcatgaca ggcactgcac tgcccctgcc agcgcggata atcggccccg
     8941 cattcattac aaacaaaggc gcgttttgga gcttttgcca cggtgtacct cgttaatgct
     9001 gtgccgcccg caggatgacg ggcaggcaat tacttctgat tcaggctgcc agagaggatg
     9061 cagaataccc ccatcaggtc agcgtgacgg atggtgactt ccgccttttc attcactttt
     9121 ggcttggcat ggtaggcaat ccccagccct gctgctttga tcatcggcag gtcattagct
     9181 ccatcgccaa tcgccacggt ctgcgccagc gggatttcat actcctgcgc gaggcgagtc
     9241 agagttttcg ctttgtactg cgcgtctacg atgtcgccga tcacattgcc ggtaaattta
     9301 ccgtccatga tctccagttc attggctacc acggctgtca ggcgcagctt gtcgcgcagg
     9361 tattcagcaa agaaagtaaa gccgccggag gcaatcgcca ctttccagcc cagcgtttcc
     9421 agcttgagca ccagctgcgt taagcctggc atcagcggca gattttcacg cacctgttgc
     9481 agaatattgg cgtcagcgcc tttcagcgtc gccacgcggc tgcgcaggct ggcggtaaaa
     9541 tcgagttcgc cgcgcatcgc ccgttcggtt acctccgcca ccatctcgcc cgttccggcc
     9601 agcttggcaa tttcatcaat acattcaatc tggatggcgg tggaatccat gtccatcacc
     9661 agcaaacccg gcgtgcgcag gtgcgggatt ttccccagcg gggcgacatc cagctgcgct
     9721 tcgtgggcca ggcgtgtagc ccgtgcggtg agtgaacctg ccagacgaat cacctgataa
     9781 tcttccacgc accaggcggc aacaatcacc atcgccgcac ccagtttgct ctggtattgg
     9841 gtcagacgct gtttatccag cccacgacca tacagcagcc agccgctacg acctgcgtgg
     9901 taatccagtg gcatcacttc atcaccactt aatgaaagag gcagacccgg ccataaagag
     9961 acatcttcag gcaggtcgca ccaggtaatg ttaggcatta aggctcctgt aaaatcgttc
    10021 gaagcaggga aaataacgca tgaggctacc ttgtatccat tgcttctggc aacattaagt
    10081 ctcaaatttt caaagggtgg aagatggctc gcacaaaact gaaattccgg ctacatcggg
    10141 cagtgattgt cctgttctgt cttgccttgt tagtggcact gatgcagggg gcgtcatggt
    10201 ttagtcaaaa ccaccagcga cagcgtaatc cacagctgga agaactggcc cgcaccctgg
    10261 cgcgtcaggt gacgctgaac gttgcaccgc tgatgcgtac cgactcaccg gatgaaaaac
    10321 gcattcaggc gatcctcgat cagttaacgg atgaaagccg tatcctcgac gcgggtgtgt
    10381 atgacgaaca aggcgatctt atcgcacgtt ctggcgaaag cgtcgaagtg cgcgatcggc
    10441 tggcgctcga cggtaaaaaa gcaggcggct attttaacca gcagattgtc gagccaattg
    10501 cgggtaaaaa cggaccgctc ggctatctgc gcctgacact cgacacccat acgctcgcca
    10561 ccgaagccca acaggtggat aacaccacta acattttacg cctgatgttg ctgctctcac
    10621 tggcaatcgg cgtagtgctg acccgcacgc tgctacaggg taaacgcacc cgctggcagc
    10681 aatcgccctt cctgttaacc gccagcaaac cggtgccgga agaggaagaa agcgagaaaa
    10741 aagagtgacc cattactaca agaaaggaaa tcgttatgtc cacattacgc ctgctcatct
    10801 ctgactctta cgacccgtgg tttaacctgg cggtggaaga gtgtattttt cgccaaatgc
    10861 ccgccacgca gcgcgttctg tttctctggc gcaatgccga cacggtagta attggtcgcg
    10921 cgcagaaccc gtggaaagag tgtaataccc ggcggatgga agaagataac gtccgcctgg
    10981 cgcggcgcag tagcggtggc ggcgcggtgt tccacgatct cggcaatacc tgctttacct
    11041 ttatggctgg caagccggag tacgataaaa ctatctccac gtcgattgtg ctcaatgcgc
    11101 tgaacgcgct cggcgtcagc gccgaagcgt ccggacgtaa cgatctggtg gtgaaaaccg
    11161 tcgaaggcga ccgcaaagtc tcaggctcgg cctatcgcga aaccaaagat cgcggcttcc
    11221 accactgcac cttgctactc aatgccgacc tcagccgcct ggcaaactat ctcaatccgg
    11281 ataaaaagaa actggcggcg aaaggcatta cttcagtgcg tttccgcgtg accaacctca
    11341 ccgagctgct gccggggctc acccatgagc aggtttgcga ggccataacc gaggcctttt
    11401 tcgcccatta tggcgagcgc gtggaagcgg aaatcatctc cccggacaaa acgccagact
    11461 tgccaaactt cgccgaaacc tttgcccgtc agagtagctg agagtggaac ttcggtcagg
    11521 ctccggcatt ctcgcatctg ctggatgaac gctttagctg gggcggcgtg gaactgcatt
    11581 tcgacgttga aaaaggccat atcacccgcg cccaggtatt taccgacagc ctcaaccccg
    11641 cgccgctgga agccctcgcc gggcgactgc aaggctgcct gtaccgcgcg gatatgctgc
    11701 aacaagagtg cgaagcgctg ttggttgact tcccggacca ggaaaaagag ctacgggagt
    11761 tgtcgacgtg gatagcgggg gcggtaaggt aactcgctga ttaaaatatc caaggaagga
    11821 aatatgatcg atctggaaaa ccaggaacgg gagattatca atttaatgtt cagccagcgt
    11881 atttcctggc tagccgccgt gcgtatcagg cataaattat cgcttgctga agtaagtaaa
    11941 atgttaggta tctcgattaa ttctctaaaa cagatcgaaa agacggaacg gcttagctca
    12001 aacatcaaaa gcaaaatggc agaaatttat ggttgtccac cagagttgct gatatgccct
    12061 tcctggatga cggctgaaca taaatgaata gattcgtgaa tagatacttg aatgtatttt
    12121 attttctatc catatgaaaa ataatagtaa atctataaat cagttgctat tgatgaatag
    12181 atactaaaaa gccggagcag tcccccactc cggcttaaca cgacaatcgc ctttcagcga
    12241 aacacaatta ctctttatcg cccagcagaa cggattccag tgcgattttg atcatgtcgt
    12301 tgaaggtggt ctgacgctca gcggcagtgg tctgctcgtg agtgcggatg tggtcagaca
    12361 cggtgcagat ggtcagggct ttcgcgccaa actctgcagc cacgccgtag ataccagccg
    12421 cttccatttc cacgccgagg atgccgtatt tttccatcac gtcgaacatt tcgccgtccg
    12481 gagagtagaa caggtcagcg gagaacaggt tacccacgcg agcatcaacg cccagtgctt
    12541 tagctgcatc tactgcgtta cgcaccatgt caaagtcagc gatagcggca aagtcatggt
    12601 ctttaaaacg gatgcggtta actttggaat cggtgcaggc ccccataccg ataacgacgt
    12661 cgcgcagttt tacgtgcggc agaactgcgc cacaggaacc aacgcggata attttcttca
    12721 cgccgaaatc ggtgatcagt tctttggtgt agatggagca ggacgggata cccataccgt
    12781 gacccattac ggaaattttg cggcctttgt aagtaccagt gaagcccagc ataccgcgaa
    12841 cgttgttcac ttcacgagca tcgtcaagga aagtttcagc aatatacttc gcacgcagcg
    12901 ggtcgcctgg catcaaaact acgtcagcga aatcgcccat ttctgcatta atgtgtgggg
    12961 tagccattgt tttatccttt ttgtgacata acaaaggcgg agtcaacgct ccgcccaaat
    13021 ccatcagaac atggctttgc catattccat atcagaagta ccaaaatatt ttgccagagt
    13081 ctggccgata tccgcgaagg tttcacggtg acccagtgag cccggtttta ctttcgggcc
    13141 atacaccagt accggaatgt gttcacgcgt gtggtcagta ccgttccagg tcggatcgca
    13201 accgtggtca gcggtgagga tcaggatgtc gtcatcgcgc agcagagaca tcagctccgg
    13261 cagacggcgg tcgaacagtt ccagacccgc agcataaccg gcgacgtcgc gacggtggcc
    13321 ccaggaagag tcgaagtcaa cgaagttggt gaagacgatg gtgttatcac ccgcttcttt
    13381 catctctttg atggtggcgt caaacagcgc gtccaagcca gtcgctttca cttttttggt
    13441 gataccgcag ttggcgtaga tgtctgcaat tttaccgaca gaaaccactt ggccgtgttt
    13501 ttcatcaacc agtttctgca gcacggtcgg tgctggcggc tcaacagcca ggtcgtgacg
    13561 gttaccggta cgctggaagt taccagcttt gtcaccgata aacggacgag cgataacacg
    13621 accgatattg tagccgccgt tggtcagctc ttcacgggcg atttcgcaca gttcgtagag
    13681 tttatccaga ccgaaagttt cttcatggca ggcaatctgg aacacggagt cagcggaggt
    13741 atagaaaatc ggcttgccgg ttttcatgtg ctcttcgccc agttgatcca gaatgaccgt
    13801 accggaagag tggcagttac cgaggtaacc cggcagatta gcgcgttcga ccagtttatc
    13861 cagcagctct tgcgggaagc tgttttcgtg atcggagaaa tatccccact caaacagaac
    13921 cgggacaccg gcgatttccc agtgaccaga cggggtatct ttaccggatg acatttcgtg
    13981 cgcccatgcg tacgcgccga taacttcagc gttgccgtcc attcccgccg gaatgaaacc
    14041 ggtagaacct tcgtgtgctt tcgccagccc cagacgggtc agatttggca gattgagcgg
    14101 ccctttacga ccattatcag cttcgccttt ggcacaagct tctgcgatat gacccagggt
    14161 gtcagccccg acgtcaccaa agcgttctgc atcttctgta gcgccgatgc cgaatgagtc
    14221 cagcaccata ataaatgcac gtttcatatg ttctccgtac tcagtgcttc aaatttaaat
    14281 gatcagatca gtataccgtt attcgctgat acggcgatag acagttggtg tgctttccgg
    14341 tgctttatcg gcaagtttaa ttgccgcttt caccgctttc gccgcttcct gccagctgtt
    14401 ttcgtctttc gctaggataa ccgctagcgg acgctgaccg tctacctggt cgcccagacg
    14461 cgccatatca gtaaagccga cgctgtaatc gatggtgtca gatgcctgac gacgaccgcc
    14521 gcccattgca accactgcca tccccagcgc gcgggtatcc atttcactga caaaaccttc
    14581 ggtatcagca tagactgctt tcgtcagcat cgctgtcggc agatacttcg cgtagttctc
    14641 aacgaagtcg gtcgggcctt tttgtgccgc taccatacga ccaaagactt ctgccgcttt
    14701 accgttgtcc agcaccgcct gcaatttcgc gcgcgcttcg gcgtcatctt tcgccagttt
    14761 gccggagatc agcatctcca cgcacagcgc catcgtgaca tcaaacagac gcgggttacg
    14821 atactcaccc gtcaggaact gcaccgcttc acgaacttca accgcgttac ctgcactgga
    14881 ggccagtacc tgattcatgt cggtgagcag cgcggtggtg cgcacgccag cgccgttagc
    14941 cacgccaaca atcgcttcgg caagggcttc agagagttcg taggtcggca taaacgcgcc
    15001 gctacccact ttcacgtcca tcaccagcgc gtccagacct tccgccagtt tcttcgccag
    15061 gatcgaggca gtgatcagcg ggatggagtc cacggttgcg gtaatatcac gggtcgcgta
    15121 gaaacgttta tcagccggag ccagtgaact ggtctgaccg ataatcgcca cgccgacgtc
    15181 tttaataatt tcgcggaaac ggttgtcatc cgggaaaatg tcgaagccag ggatggattc
    15241 cagtttgtcg agcgtaccgc cagtatgacc gaggccgcaa ccggagatca tcggaatata
    15301 gccgccgcag gctgcgacca tcggccccaa catcagcgaa gtgacatcgc cgacgccgcc
    15361 ggtcgagtgt ttatcaacaa tcgggccatt cagatgcagg cttttccagt cgagaacggt
    15421 tcctgaatct cgcatcgcca tggtcagcga gacacgctca ggcattgtca tatcgtggaa
    15481 gaaaatggtc atcgcgaggg cggcaatctg cccttcggaa atagtgttgt cgcgaatacc
    15541 gttgataaag aaacgaattt cttcatcgct cagcgcatga ccatcacgtt ttttacgaat
    15601 aatttcttgt gcgagaaaca aggtaacctc ccgaggaaaa gagtatgagg cggcttttgc
    15661 cggatgcgtc tacgccttat ccggcctacg ggtagatacg tctcgtaggc ctgataagcg
    15721 cagcgcatca ggcgtaaagc atcttactta gtagctgctg gcgctcttac cgtcgccgtg
    15781 gcccagcgct ttcagcaggc ttgccagcag gctggaagca ccaaagcggt agtgacgcgc
    15841 atcagcccag tcagcaccga acagttcatc tgcgatggcg agatatttct gcgcatcttc
    15901 cgcagtacgc acgccgcccg ccggtttgaa accaacggtt ttttctacgc ccatatcacg
    15961 gatcacttcc atcatgatgc gagcgctttc cggcgtcgcg ttcacagcca ctttaccggt
    16021 agaggttttg atgaagtccg cacccgcttt gatggagatt tcagacgctt tacggatcag
    16081 cgcttcgtct ttcagttcgc cggtttcgat gatcactttc agcagtacat tcgctgccgc
    16141 gcaagcctct ttacaggctt tcaccaggtc aaaaccaacc tgctcgttac ccgccatcag
    16201 cgcgcggtac gggaacacca cgtcaacttc atcagcaccg taggcgattg ccgcacgggt
    16261 ttctgccagc gcgatttcga tgtcgtcgtt accgtgtggg aagttggtta ccgtagcgat
    16321 acggatttcc ggggtgccct gctctttcag agttttgcga gcaatcggga taaaacgagg
    16381 atagatacag atagcggcgg tattgccgac cggagttttg gcctgatgac acagagcaat
    16441 tactttctcg ttggtgtcgt cgtcattcag ggtggtcagg tccatcaatt tcagtgcacg
    16501 caggctgctt gctttcagat cagtcatttc attctcctgg cttgtcgcca ataaaattca
    16561 ccttgcgagt ttgttagtat tctaacatct actccgcaac acacttcgat acacatcaca
    16621 attaaggaaa tctacttaca agtttgcatc actgtaatgc gatctggttc aaataattca
    16681 ctttcaaatg aatgcgtcag tggtggcaaa cgcatcagga tcaaagtgaa catcacgaaa
    16741 cttcttacaa tggcgcatca ccttcggcat aaaaggaaaa gaagatgccc acttctcatg
    16801 aaaatgcact gcaacaacgt tgccagcaaa ttgtcaccag cccagtgctt agcccggagc
    16861 agaagcgcca ttttctggca ctggaagcag aaaacaatct gccttaccca cagcttcctg
    16921 ccgaagcccg ccgcgcgctg gatgaaggtg taatctgcga tatgtttgaa ggtcatgcgc
    16981 cgtacaaacc gcgctatgtc ttacccgatt acgctcgttt tctggcgaac ggttccgaat
    17041 ggctggagct ggaaggcgcg aaagatcttg atgacgcact ctctctgctg accattcttt
    17101 accaccacgt accgtcggtc acatcgatgc cggtctacct ggggcaactg gatgcgttgt
    17161 tgcaaccgta tgttagaatt ctaacacaaa acgagatcga tgttcgaata aaacgtttct
    17221 ggcgttacct cgacagaacc ctgccagatg cctttatgca cgcccgtccc gtctgattcg
    17281 cccattaccc gcgcgatctt acgtgcagac gcgcaggttt caccgaacct gacctttatc
    17341 tacgatcctg aaatcacccc tgatgacctg ctgctggaag tggcgaagaa catctgtgaa
    17401 tgtagcaaac cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaatggggc
    17461 tacgggattg tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta
    17521 cgccttaacc tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc
    17581 actctaccgc actactgcca gcagcagatc gccatcatcg atgcgcggtg tgaattcctc
    17641 tatcaacaat cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct
    17701 gaacgttttg tgccaatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt
    17761 gagaaagaag ggattgccgc gcgctacggt aaagaagccg ccgcaaatga agtgggttat
    17821 cgcatcagcg cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa
    17881 aaacgcgcca tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc
    17941 gcgcgtttgc cgtatggcga tgagccagat ccgatcaccc atctgcaaac tgtcgccccg
    18001 catcatgctt attattattc cggcatcagc gacattctga cgctcgacga aatcatcaaa
    18061 cgtaacccgc aggcactggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa
    18121 tttaccgcca atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg
    18181 tcggatttag aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa
    18241 gaagccgcac gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag
    18301 cagatgcgct ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg
    18361 tctggctctg tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac
    18421 gatgggacgt tgcaatggct gtggggagtg cgtgccacag tgtccgcatc aggcgttgca
    18481 gattgttgac ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct
    18541 gaagatatgt ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct
    18601 tagccatgtg cgcaaagcgg tgctgtttat tgaagggata acggtaagcg gcggcgaagc
    18661 cacgacccaa ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact
    18721 gcgccatctc acctgtctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa
    18781 attactcccg gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca
    18841 tcaacaatta accggacgcg ataatcaaca gattaagcgc agcatctatt tgctggcaga
    18901 gcgcggcaag ctggcggaac tgcgtttgct gatgatccca ggccaggtgg attatttgca
    18961 acacatcgaa gaactggcgg cgtttatcaa gggacttggc gatgttccgg ttcgcctgaa
    19021 cgcgtttcat gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga
    19081 agacgttgag ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc
    19141 ggcgctctat ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcctg
    19201 ataagacgcg tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac
    19261 gccttatccg gcctacggtt cggcacaaac ctgtaggcct gataagacgc gttaagcgtc
    19321 gcatcaggca ttatgcgccg actgccggat gctgcctacg aagggcaaac tacccacgaa
    19381 cgttaaacac cgcatacgtg ttattaagca acacttcggc aatctcatcc gcgggttccg
    19441 ggcgtaactc gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc
    19501 cctgaaaacc gttgagcggc atatccggcg catcggtttc cagcaacaac gatgccagcg
    19561 gtaattttgc gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtaccgccta
    19621 cgccgatttt gtagcccagc tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc
    19681 cgtggacaac accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt
    19741 gcgtgcgccg tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt
    19801 cgtcgagtaa ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga tccagaccga
    19861 tctcccccac cgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgctcaa
    19921 gagacacatc gctatgtttt tccaacatac caggatgcaa gcccaatgcg gcatacagcg
    19981 gttgataatt ttccgctaac gcctgcacgc gggcaaaatt ttccgcctct gtcgccggaa
    20041 caatgatctt gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa
    20101 taaaaggcgg gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc aagccagatc
    20161 ctcattgtta aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt
    20221 atcgttggcg actggcgcag gcggaatgac aatcgattcc ggcgtcacca ctggcaccag
    20281 atggcgggta agcggcgctt tttcagttaa tagcttgcca accgtggcga ggaaataacg
    20341 accgcataaa cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc
    20401 gatactatgt aatggcttcg gcggataaat ttcgaatatc cgcagcttgc ccggtggttt
    20461 ctcaataaat tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag
    20521 cggctgcagg ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta
    20581 gtacatttgt gacggcacag tgcggatgac gaccaacgtt ttagcgccct gccttgccgc
    20641 ctctttaacc ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga
    20701 cactccgcta cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt
    20761 ttgtttggtt ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg cgcacatata
    20821 aaacgatttg ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct
    20881 tgcggtggcc tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa
    20941 tggatcgaaa aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg
    21001 ctgattgcag ataaacgccg agaggttctg cgccccggca gatgtgccga gataaagatc
    21061 gaaaggatta aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg
    21121 ctgtccgccg ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa
    21181 cggcgcaata ttaccaagcg ttacaggtat tcgctgcccc acttctgcct tcctgttttt
    21241 gttgtgaaag aaagacaaaa taacgcaatt tattattcgc taaaaccgca aaagccagtc
    21301 cgctggactg gctttggata aaattttctt aatggtctaa aatctagggt cgttttcggc
    21361 ccatgaacaa actcaccagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc
    21421 ctgcagcggt accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca
    21481 gaaatatgat gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt
    21541 gatatgagcg tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc
    21601 gacaaagttc gggaggacga attacgacga attacttagt tttcagatca tttttaacgc
    21661 ttttcacacc atctaccgct ttggcgatac tttcagcacg gtcactttgt gcctgagaat
    21721 ctacggtacc ggagagctga accacgccat cggtggtttc aactttcact ttacgtgaag
    21781 ggacgatatc gtccgccagc agtttggctt tgatttcact ggtggtggcg gtgtcacccg
    21841 cgtagccctt caccgagcct tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg
    21901 tcacgccttc aacgcctttc gccactttca ctgcctcttc ggcctgggcc tgactttcaa
    21961 cgaaaccgct cagggtcacg actttttgat cggttttgac agagatatcg gtgctcttga
    22021 tgttgtcatg atccaccagc gccgccttca ctttcgcggt gatggcgctg tcatccatga
    22081 aattaccgac tttattcata gagctatcga ctttttgccc tgcgctttca ttggtagtct
    22141 gcgcgttgtt ttccgcgtag gcagagccgg tcgcgacggc agaggtcaac attacagcca
    22201 gcagagtttt cgaaatcttc agtcttgtca tagtcatcga tttattcctg tatgtttgct
    22261 cgtaatttga gcctggcaac acgaggttgc attgctgaat agggagagac ttcaccctct
    22321 acagaagtca atggtcgcca tcacaaaagc gatgggtgat gaataacgac cattacagcc
    22381 tctgaatcag ttattaatat cggtagaatg acaatcgacg gctttagata ctgatatcta
    22441 cgcattgaac ggtatttaac gccatcagaa atgtcatcac tttgttaaat atagatcaca
    22501 attttgaaac cgctcgggat atcagcgaaa acataagcaa aagtgaatgt tttaagaaca
    22561 ttccgtaagc ggctaataag gaagggaaat tgacagggcg cagcggctac cgcgccctgg
    22621 aggcaagaat taatgctcac gggtctggtg gaactgaacg tccggataac gttcctgtgc
    22681 caggcgcagg ttgaccatgc tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc
    22741 cagttggctt tcgttcttac gcttgaactc ttcgaatttc ttcgcgtctg cacattctac
    22801 ccagcgggca gtggcaacgt tgactgactc atacactgct tcaacgttgt attcgctctt
    22861 caggcgcgct accaccacat caaactgcag cacaccaacc gcaccaacga tcagatcgtt
    22921 gttggagatt ggacggaaca cctgcaccgc gccctcttcg gaaagctgta ccagcccttt
    22981 gagcagctgt ttttgcttca gcggatcttt caggcggata cgacggaaca gttctggtgc
    23041 gaagttcgga ataccggtga acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg
    23101 aatggtgccg tggttgtgca ggccgaggat atcgcccgga tacgcttctt caacgtgcga
    23161 acggtcaccc gccataaagg tcagcgcgtc ggagatcacc acatctttcg cagtgcgcac
    23221 ctggcgcagt ttcatgcctt tttcatattt accggacacc acacgcataa acgccacgcg
    23281 gtcgcggtgt ttcgggtcca tgttggcctg aattttaaat acgaagccgg taaatttatc
    23341 ttcgctcgct tctacggtac gggtatcggt ctgacgcggc atcggcgcag gcgcccactc
    23401 caccaggcca tccagcatat gatcaacgcc gaagttaccc agcgcagtac cgaagaatac
    23461 cggagtgatt tcgcccgcaa ggaacagctc tttgtcgaac tcgttagacg cgcctttaac
    23521 cagttccagt tcgtcacgca gctgctgtgc cagatcttca ccaaccgcag catcgagatc
    23581 cgggttattc agccctttaa caatgcggac ttcctgaatg gtgtgccctt taccgctctg
    23641 atagagatag gtttcatctt tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc
    23701 aattggccag gtgatcggtg cgcagccaat tttcagctcg ttctcaactt catcgagcag
    23761 ttccatcggg tcgcggatat cacggtcaag tttgttcata aaggtgagga tcggcgtgtc
    23821 gcgcagacgg gtaacttcca tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc
    23881 gataaccatc aggcagcagt ccaccgccgt cagggtacga taggtatctt ccgagaagtc
    23941 ttcgtgcccc ggggtgtcga gcaggttaac caggcaatcg tgatacggaa actgcatcac
    24001 agacgtagta atggagatcc cacgctgctt ttccatctcc atccagtccg acttagcgtg
    24061 ctggttggaa ccacggcctt ttactgtacc ggcggtctga atggcctgtc cgaacagcag
    24121 caccttctcg gtgatggtag ttttaccggc gtccgggtga gaaataatgg caaaagtgcg
    24181 gcgcttcgcc acctcttgca aataaggaga caacgtcata attctttctt cttgagtaag
    24241 cggcatcgcg ccgcgcttgt tgaaaacgaa aaattgcggc tattttaccc atcaacgggg
    24301 gggaggcaat cagtgtttac acaggagctg ctccagttcg tgcaacgaag aaacggtcca
    24361 ggtgggcgcg atgccttctg gttgctcgcg attgtgcgca ttcagccagc aggtcgcaag
    24421 cccggcgttg atgccaccga gaatatcgga ctcggcagtg tcgccaacca tcagcacgcg
    24481 tgaacggtca ggattgcccg cctgttccag cgcataatcg aaaattttct tattcggttt
    24541 ggcaacgcca acttcttcgg aaatcaccag caaatcgaag taatcacgca ggcccgtccg
    24601 ttccagacgc acttgctgca aggcgctaaa accgttggtg atgatgccga ttttagcgtt
    24661 gccacgaatg gcgttaagca gagaaaccgc gcccggcaac ggcgtacaga tttccgccat
    24721 cgcattaata aaggcatcgt taagcaagcc tgcttcaact ttcagccgtt cggcccagct
    24781 ctcgaaacgc ccgtgctgaa gctgtaatga agtgatcgcg ccgttttgat aatccaccca
    24841 cagtggcttg ttaacggcct gatagtcctg aaaatcttca gcggtaaagg tgacgctgta
    24901 atcaagaaac atccgctgca ggccggtgaa tgagtcaaag gtaaacagcg tttcatcggc
    24961 atcaaagaaa atccagtccc acttcattat tccaccttgt cttacatact gattggcaac
    25021 gccatgatga tggcgtcttc gcgaccgtcc gtggtggggt agtaattgcg gcgaatcgtc
    25081 gcctcgttaa agcctaaact ttcgtacagg gcaatggcgg cagcatttga ggcacggact
    25141 tccagccata gtgtcgccac gccgcgtttt tccagttcgt cgatcagatg ttccagcagc
    25201 gccctaccca gcccctgacg ctgatagtcg ggatcgaccg caatattgaa caatgtagct
    25261 tcatccagca ccacttgcgt aatcgcaaac gccgccattt tgccgttttg cgttaactga
    25321 aagttgagat aacgctcgcc ctggttgctg gcaaaggttt tttcactcca cggaaaggcg
    25381 tgggcgcgtt gttcaatgtg gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc
    25441 gtgttcatat gtgcaaattt gttgccataa cgcggcgcgt gccgttgggt ttgcccgtaa
    25501 ttcggtgagc gccggtgatg ccacctgagc gccttccaat gatagcggtt cgtcagtacc
    25561 caaccgccag ctgttgcagc gactgccttg cggcagcatc gcgatttttt ctggcgtcag
    25621 ttgcagcact tgatcggggc tgacggttaa tgcgcgcaga acatcgctca ctaaaggatc
    25681 agtcagggcg ggaagatcgt ttgccaccat caccagacgg acgtgtgccg ggctcgcaat
    25741 ggcaatctcc ccctgcaacg cgccagggcg acgcagcgac cactgggtaa tgcccagttg
    25801 ctgtaactgc cagtctcgtc gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa
    25861 atatagcaaa ttcgtcgata ccgcgccaac atatggctat aatcgccgcc agtatcaatt
    25921 gaggagcatt ccatgtctgc atttaccccg gcaagtgaag tcttgctgcg tcacagtgat
    25981 gatttcgaac aaagccgtat tctgtttgcc ggagacttac aggatgacct gcccgcgcgt
    26041 ttagataccg cggccagccg tgctcatacc cagcaattcc accattggca ggtgttaagc
    26101 tgccagatgg gggataacgc ccgttttagt ctggtcgcca cggcgaatga tgtcgcagat
    26161 tgcgatacgc tgatttacta ctggccgaag aacaaaccgg aagcccagtt ccagttgatg
    26221 aatttacttt ctctgctgcc agtggggaca gatatttttg tcgttggcga gaaccgcagc
    26281 ggcgtgcgca gcgctgagca gatgctggca gattatgcgc cgttgaataa agtcgacagc
    26341 gctcgtcgct gtggcctcta ttttggtcgt ctggaaaaac agccagtatt tgatgcggat
    26401 aaattctggg gcgaatacag cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt
    26461 agccgcgacg gtctggatgt cggtagccag ttgctgctct cgacgttaac tccgcacacg
    26521 aaaggtaaag tgctggatgt cggctgtggc gcgggtgtac tttcagttgc ctttgcgcgc
    26581 cattcgccga aaattcgtct caccttgtgc gatgtctctg ccccggcggt agaagccagc
    26641 cgcgcaacac ttgcagccaa cggtgttgaa ggtgaagtct ttgccagcaa cgtcttttcc
    26701 gaggtgaaag gtcgttttga tatgatcatc tccaacccgc cgttccacga tgggatgcaa
    26761 accagcctgg atgcggcgca aacgctgatt cgcggcgcgg tgcgtcatct taatagcggc
    26821 ggcgagctgc gaattgtagc gaacgccttc ctgccttacc cggacgtgct ggatgagaca
    26881 tttggcttcc atgaagttat cgcgcaaacc gggcgtttca aggtatatcg cgccattatg
    26941 acccgccagg cgaagaaagg ttgagtatct gatatctgtc gccgcatacc gtgtcggatg
    27001 cggcgtgaac gccttatccg acctacaaaa cctgcacgtt agcccttcgt aggccagata
    27061 agacgcgcca gcgtcgcatc tggcatctgc ccccgcatgc ctaccaaacc tgcacgctag
    27121 tttcctgatg gacatttttc cagcaattac acatcaggcg ataattaact attgacgaaa
    27181 agctgaaaac cactagaatg cgcctccgtg gtagcaattc tttttaagaa ttgatgg
//
LOCUS       DBKEKI010000050        25155 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.050, whole genome shotgun sequence.
ACCESSION   DBKEKI010000050 DBKEKI010000000
VERSION     DBKEKI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.050"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            68..505
                     /locus_tag="ACUQPX_002040"
                     /pseudo
     CDS             68..505
                     /locus_tag="ACUQPX_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
     gene            complement(537..1373)
                     /gene="yeiG"
                     /locus_tag="ACUQPX_002041"
     CDS             complement(537..1373)
                     /gene="yeiG"
                     /locus_tag="ACUQPX_002041"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HHV9056757.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            1631..2299
                     /gene="folE"
                     /locus_tag="ACUQPX_002042"
     CDS             1631..2299
                     /gene="folE"
                     /locus_tag="ACUQPX_002042"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HHV9056758.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            2316..3473
                     /gene="yeiB"
                     /locus_tag="ACUQPX_002043"
     CDS             2316..3473
                     /gene="yeiB"
                     /locus_tag="ACUQPX_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HHV9056759.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            3615..4655
                     /gene="galS"
                     /locus_tag="ACUQPX_002044"
     CDS             3615..4655
                     /gene="galS"
                     /locus_tag="ACUQPX_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HHV9056760.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AVAITNSTNQAM"
     gene            4935..5933
                     /gene="mglB"
                     /locus_tag="ACUQPX_002045"
                     /pseudo
     CDS             4935..5933
                     /gene="mglB"
                     /locus_tag="ACUQPX_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
     gene            5994..7514
                     /gene="mglA"
                     /locus_tag="ACUQPX_002046"
     CDS             5994..7514
                     /gene="mglA"
                     /locus_tag="ACUQPX_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HHV9056761.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            7530..8540
                     /gene="mglC"
                     /locus_tag="ACUQPX_002047"
     CDS             7530..8540
                     /gene="mglC"
                     /locus_tag="ACUQPX_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HHV9056762.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDTTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLVAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8611..9846)
                     /gene="preA"
                     /locus_tag="ACUQPX_002048"
     CDS             complement(8611..9846)
                     /gene="preA"
                     /locus_tag="ACUQPX_002048"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HHV9056763.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(9840..11078)
                     /gene="preT"
                     /locus_tag="ACUQPX_002049"
     CDS             complement(9840..11078)
                     /gene="preT"
                     /locus_tag="ACUQPX_002049"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HHV9056764.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(11272..11511)
                     /locus_tag="ACUQPX_002050"
     CDS             complement(11272..11511)
                     /locus_tag="ACUQPX_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HHV9056765.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(11514..12233)
                     /gene="sanA"
                     /locus_tag="ACUQPX_002051"
     CDS             complement(11514..12233)
                     /gene="sanA"
                     /locus_tag="ACUQPX_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HHV9056766.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(12383..13267)
                     /gene="cdd"
                     /locus_tag="ACUQPX_002052"
     CDS             complement(12383..13267)
                     /gene="cdd"
                     /locus_tag="ACUQPX_002052"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HHV9056767.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(13397..14092)
                     /locus_tag="ACUQPX_002053"
     CDS             complement(13397..14092)
                     /locus_tag="ACUQPX_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HHV9056768.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(14089..14487)
                     /locus_tag="ACUQPX_002054"
     CDS             complement(14089..14487)
                     /locus_tag="ACUQPX_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HHV9056769.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYILIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            14726..15599
                     /gene="dusC"
                     /locus_tag="ACUQPX_002055"
                     /pseudo
     CDS             14726..15599
                     /gene="dusC"
                     /locus_tag="ACUQPX_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
     gene            complement(15970..16053)
                     /gene="yohP"
                     /locus_tag="ACUQPX_002056"
     CDS             complement(15970..16053)
                     /gene="yohP"
                     /locus_tag="ACUQPX_002056"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HHV9056770.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            16276..17712
                     /gene="mdtQ"
                     /locus_tag="ACUQPX_002057"
     CDS             16276..17712
                     /gene="mdtQ"
                     /locus_tag="ACUQPX_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HHV9056771.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17804..18526
                     /locus_tag="ACUQPX_002058"
                     /pseudo
     CDS             17804..18526
                     /locus_tag="ACUQPX_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(18643..19221)
                     /locus_tag="ACUQPX_002059"
     CDS             complement(18643..19221)
                     /locus_tag="ACUQPX_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HHV9056772.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            19391..19978
                     /locus_tag="ACUQPX_002060"
     CDS             19391..19978
                     /locus_tag="ACUQPX_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0016192 - vesicle-mediated transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HHV9056773.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            20152..21084
                     /gene="pbpG"
                     /locus_tag="ACUQPX_002061"
     CDS             20152..21084
                     /gene="pbpG"
                     /locus_tag="ACUQPX_002061"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HHV9056774.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(21122..22837)
                     /gene="dld"
                     /locus_tag="ACUQPX_002062"
     CDS             complement(21122..22837)
                     /gene="dld"
                     /locus_tag="ACUQPX_002062"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HHV9056775.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            23033..>25155
                     /gene="bglX"
                     /locus_tag="ACUQPX_002063"
     CDS             23033..>25155
                     /gene="bglX"
                     /locus_tag="ACUQPX_002063"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HHV9056776.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKG"
BASE COUNT         6219 a         6267 c         6615 g         6054 t
ORIGIN      
        1 tctaaaaact agtattaaga ctatcactta aataagtgat actggttgtc tggagattca
       61 gggggccagt ctatacaacg ttaacaaagc tcgtattcag ggcgtggaaa ccgaactgaa
      121 aattccgttc aacgatgaat ggaaactgtc gatcaactac acctacaacg atggtcgtga
      181 tgtcagcaac ggcgaaaaca aaccgctatc cgatctgccg ttccatactg ctaacggtac
      241 gctggactgg aaaccgctgg cgctggaaga ctggtcattc tatgtttctg gtcactatac
      301 cgggcagaaa cgcgccgaca gcgcgacggc taaaacaccg ggcggttata ccatctggaa
      361 taccggcgcg gcctggcagg tgactaaaga cgtcaaactg cgcgcaggcg tgctgaacct
      421 tggcgacaag gatctcagtc gtgacgacta cagctataac gaagacggac gtcgttactt
      481 tatggcagtg gattatcgct tctgatgaga agatgcccgg cgaaccgggc ggactttcac
      541 ttcagtaaat actgtgcatg gaagcgcagg tgatcctcta taaaagaggc gatgaagtag
      601 taactgtgat catatcccgg ctgaatacgc agcgtcatcg gccacgcttt ctggcgcgcg
      661 gcttctgcca ataccgcagg ttgcaactgg tcggcaagaa actgatcatt atcgccctga
      721 tcgataagcg tcgggatcgc atcctgcgcg ttactggcat acatcagcgc gcaactatcc
      781 cattccagcc atgcattttt gtcctcacct aaataggtgc taaacgcttt gattccccac
      841 gggacgctgc acggattcac aatgggcgca aaggccgaaa cgctggtgta tttacccgga
      901 tttttcagcg ccataatcag cgcaccgtga ccacccattg agtgaccgct aatggcgcag
      961 cggtcgctga cattaaattg cgactgaacc agcgccggta attcatcgcg cagataatca
     1021 tacatccggt aatgcgtcgc ccacggcggt tgcgtggcat taagataaaa gcctgcgccc
     1081 tggcctaaat cgtagccatc gtcgttggca accttttcgc cgcgcgggct ggtgtctggc
     1141 atcaccagta caatccccag ttccgccgct acccgctggg caccagcctt ggtggtgaag
     1201 ttctcgtcat tgcaggttaa tccggaaagc cagtacagca ctggtggcgg ggtgtgatca
     1261 cgagggggtg ggagaaagat actgaacgtc atcgggcagt ttaaggtact ggagtcgtgt
     1321 ctccagcgtt gctgccagcc ttcaaaacag cggtgctctt cgagcatttc catgcgtggc
     1381 tccttgttgt gttgtttgca atttcttcat cataccgttt attcaatgtg ctgtgagtaa
     1441 ctttcacttc cgtatttgca taacgatgtt ttaacatctg ctgatgaaag gcagcggcaa
     1501 ttacaataat tatcgctgtg aatactggat tatgtgcgcc gcctcacgca caataatcaa
     1561 gctgtaaatc agcttaataa ctttgccccc acgcagggcg gaggcgtcac acctgcagga
     1621 gaaatcataa atgccatcac tcagtaaaga agcggccctg gttcatgaag cgttagttgc
     1681 gagaggactg gaaacaccac tgcgcccgcc cgtgcatgaa atggataacg aaacgcgcaa
     1741 aagccttatt gctggtcata tgaccgaaat catgcagctg ctgaatctcg acctggctga
     1801 tgacagtttg atggaaacgc cgcatcgcat cgctaaaatg tatgtcgatg aaattttctc
     1861 cggtctggat tacgccaatt tcccgaaaat caccctcatt gaaaacaaaa tgaaggtcga
     1921 tgaaatggtc accgtgcgcg atatcactct gaccagtacc tgtgaacacc attttgttac
     1981 catcgatggc aaagcgacgg tggcttatat cccgaaagat tcggtgatcg gtctgtcaaa
     2041 aattaaccgt atcgtgcagt tctttgccca gcgtccgcag gtgcaggaac gtctgacgca
     2101 gcaaattctt attgcgctac aaacgctgct gggcaccaat aacgtggctg tctcgatcga
     2161 cgcggtgcat tactgcgtga aggcgcgtgg catccgcgat gcaaccagtg ccacgacaac
     2221 gacctctctt ggtggattgt tcaaatccag tcagaatacg cgccacgagt ttctgcgcgc
     2281 tgtgcgccat cacaactgat taaaaggcag gaaccatgga gcgcaacgtc acgctcgatt
     2341 ttgttcgcgg cgtcgccatt ctggggatcc tgctattaaa catcagcgcc tttgggctac
     2401 caaaggcggc ttatctcaat cccgcctggt acgatgctat tacgccgcag gatgcatgga
     2461 cctgggcatt tctcgatctc atcgggcagg tgaaattcct cacgcttttt gcgctgctgt
     2521 ttggtgctgg cctgcaaatg ttgctgcccc gtggcagacg ctggatccag tcgcggttaa
     2581 cgctgttagt cttgttgggc tttattcacg gtttattgtt ctgggacggc gatattctgc
     2641 tggcttacgg gctggtgggc ttaatctgct ggcggctggt gcgcgatgcg ccatcggtaa
     2701 aaagcctgtt taataccggc gtcatgcttt atctggtggg gcttggcgtt ttgctgttat
     2761 tggggctgat ttccgacagc cagaccagcc gcgcctggac gccggatgca tcggctattt
     2821 tgtatgaaaa atactggaag cttcacggcg gcgttgaagc gatcagtaat cgtgccgatg
     2881 gtgttggcaa cagtttactg gcactgggcg cacagtatgg ctggcaactg gcagggatga
     2941 tgctcattgg tgcggcgctg atgcgcagcg gctggctgaa agggcagttc agcttacgtc
     3001 actatcgtcg tactggtttt gtgctggtgg cgattggggt gaccattaac cttcctgcca
     3061 tcgccctgca atggcagctg gactgggcgt atcgctggtg tgccttctta cttcaaatgc
     3121 cgcgggaact gagtgcgccg tttcaggcga ttggctatgc gtcgctgttt tatggcttct
     3181 ggccgcaatt gagccgcttt aagctggtgc ttgcgatcgc ctgcgtcgga cggatggcgc
     3241 tgaccaacta tctattgcaa acgctgattt gtaccacgct tttttaccac ctcggtttgt
     3301 ttatgcattt tgaccgcctg gagctgctgg cgtttgttat tccggtatgg ctggcgaata
     3361 tcctcttctc tgttatctgg ctgcgttact tccgccaggg gccggtggaa tggctctggc
     3421 gtcagttaac tttgcgtgct gccggaccgg caatatctaa aacatcaaga taacgatctg
     3481 gatcacattc gttaacaaaa cggctgtaac cgtttccatt gctgtgactc gattcacgaa
     3541 gtcctgtatt cagtgctgac aaaatagccg ccagcaagca gtcatttact gcaatctcat
     3601 aacaggtagt gaatatgatc accattcgtg atgtagcgcg tcaggctggc gtctctgtgg
     3661 caacggtttc ccgggtgctc aataacagca cgctggtcag tgccgacacg cgtgaagcag
     3721 taatgaaagc cgtgagtgag ctggattatc ggccaaacgc caatgcccag gcgctggcaa
     3781 ctcaggttag cgacaccatt ggcgtggtgg tgatggacgt ttctgatgcg tttttcggcg
     3841 cgctggtaaa agcggtggat ctggtcgccc agcagcatca gaaatacgtg ctaatcggca
     3901 atagctatca tgaagcggaa aaagagcgtc acgccattga ggtgttaatt cgccagcgtt
     3961 gtaatgcgtt gattgttcac tcaaaagcat tgagtgacga tgaactggca caatttatgg
     4021 ataacattcc cggtatggtg ttaatcaacc gcgttgtgcc ggggtacgcc catcgttgcg
     4081 tttgcctgga taatctcagc ggtgcccgaa tggcgacgcg catgttgctg aataacggtc
     4141 atcaacgtat tggttatctt tcttccagcc acggcattga agatgacgcc atgcgtaaag
     4201 caggctggat gagtgcgttg aaagagcagg atattattcc gccggaaagc tggattggca
     4261 ctggtacgcc ggacatgccg ggcggtgagg cggcgatggt tgaactgctg gggcgcaatc
     4321 tacaacttac cgctgtattt gcttataacg acaatatggc cgctggcgca ctgacagcat
     4381 taaaagataa tggcattgcg attccgttac atctctcaat catcggtttc gatgatattc
     4441 ccatcgcccg ttacaccgac ccgcaattaa cgaccgtgcg ttatcccatt gcttcaatgg
     4501 ctaaattagc caccgaactg gccttgcagg gggcagcagg caatattgat cctcgtgcca
     4561 gccactgttt tatgccgacg ttagtgcgtc gccattctgt cgcaacgcgc cagaatgcgg
     4621 tggcgatcac taactcaaca aatcaggcga tgtaaccgct ttcaatctgt gagtgatttc
     4681 acagtatcct aacaatgtga tagctatgat tgcaccgttt taacgttgta acccgtatgt
     4741 aacagtgaat aatcactttc accgaggtaa tagcgtcata acaacaatta aagccgtttt
     4801 ctggagcgtt accgggcatg gaagaacgaa ttttaaaaag tgagcttcgg cgttcagtaa
     4861 cacttcatta actctactgc cccgccgagc atttatctca agcactaccc tgcataagaa
     4921 aaaccggaga taccatgaat aagaaggtgt taaccctgtc tgctgtgatg gccagcatgt
     4981 tattcggtgc cgctgcacac gctgctgata ctcgcattgg tgtaacaatc tataagtacg
     5041 acgataactt tatgtctgta gtgcgcaagg ctattgagca agatgcgaaa gccgcgccag
     5101 atgttcagct gctgatgaat gattctcaga atgaccagtc caagcagaac gatcagatcg
     5161 acgtattgct ggcgaaaggg gtgaaggcac tggcaatcaa cctggttgac ccggcagctg
     5221 cgggtacggt gattgagaaa gcgcgtgggc aaaacgtgcc ggtggttttc ttcaacaaag
     5281 aaccgtctcg taaggcgctg gatagctacg acaaagccta ctacgttggc actgactcca
     5341 aagagtccgg cattattcag ggcgatttga ttgctaaaca ctgggcggcg aatcagggtt
     5401 gggatctgaa caaagacggt cagattcagt tcgtactgct gaaaggtgaa ccgggccatc
     5461 cggatgcaga agcacgtacc acttacgtga ttaaagaatt gaacgataaa ggcatcaaaa
     5521 ctgaacagtt acagttagat accgcaatgt aggacaccgc tcaggcgaaa gataagatgg
     5581 acgcctggct gtctggcccg aacgccaaca aaatcgaagt ggttatcgcc aacaacgatg
     5641 cgatggcaat gggcgcggta gaagcactga aagcacacaa caagtccagc attccggtgt
     5701 ttggcgtcga tgcgctgcca gaagcgctgg cgctggtgaa atccggtgca ctggcgggca
     5761 cagtactgaa cgatgctaac aaccaggcga aagcgacctt tgatctggcg aaaaacctgg
     5821 ccgatggcaa aggtgcggct gatggcacca actggaaaat cgacaacaaa gtggtccgcg
     5881 taccttatgt tggcgtagat aaagacaact tggctgaatt cagcaagaaa taagactgat
     5941 tgggcgcagt cgtttactgc gcccttattg tatggccaac aaggtaaaat tatatggtca
     6001 gctcaacgac tccgtcttcc ggggaatact tgttggaaat gagcggtatc aacaagtcct
     6061 ttcctggtgt taaggcactt gataacgtta atttaaaagt ccggccacat tctatccatg
     6121 cattaatggg ggaaaacggt gcaggaaaat cgacattatt aaaatgcctg tttggtattt
     6181 atcaaaaaga ctccggcacc attttattcc agggtaaaga gatcgatttc cattccgcca
     6241 aagaggcact ggaaaatggt atttcgatgg tacaccagga gttaaacctg gtattacaac
     6301 gttcggtgat ggataacatg tggctggggc gatatcccac caaaggcatg tttgtcgatc
     6361 aggacaaaat gtaccgcgaa accaaagcga tttttgatga actggatatt gatatcgatc
     6421 cgcgtgcgcg cgtcggcaca ttatccgttt cgcaaatgca gatgatcgaa atcgccaaag
     6481 cgttttccta taacgcgaaa attgtgatta tggatgaacc gacttcttcg ttaaccgaaa
     6541 aagaggtcaa tcatctgttc actattattc gtaaattaaa agagcgcggc tgcggtattg
     6601 tttatatctc gcataaaatg gaagaaatct tccagttatg tgatgaagtt accgtattgc
     6661 gcgacggtca gtggatcgcc accgaaccgc tggcaggact gacgatggac aagatcatcg
     6721 ccatgatggt tgggcgttct cttaatcagc gtttccctga caaagaaaac aagccgggag
     6781 aagtcatcct cgaggtgcgt aacctaacgt cactgcgcca gccgtcgatt cgcgatgtct
     6841 cgtttgatct gcataaaggg gagatcctcg gtattgccgg gctggtgggg gcgaaacgta
     6901 ccgatattgt tgagacgtta tttggtattc gcgagaaatc ggctggcacc attacgttgc
     6961 acggcaaaca gatcaataac cataatgcca acgaagccat aaaccacgga tttgcactgg
     7021 taactgagga gcgccgctca acgggaattt atgcctatct ggatattggt tttaactcgt
     7081 taatttccaa tattcgcaac tacaaaaata aagttggttt actggataac tcgcggatga
     7141 aaagcgatac ccagtgggtg attgattcga tgcgggtaaa aacgccgggt catcggacgc
     7201 aaattggttc gctctccggt ggtaatcagc aaaaggtgat tattggtcgc tggctactaa
     7261 cgcaaccaga aatattaatg ctcgatgaac cgacgcgcgg tattgatgtc ggggcgaagt
     7321 ttgaaattta tcagttaatt gccgaactgg cgaagaaagg caaggggatt attattatct
     7381 cctctgaaat gcctgagttg ttagggataa cagaccgtat tctggtcatg agcaatggtc
     7441 tcgtttccgg aattgtcgat acaaaaacaa caacgcaaaa cgaaattctg cgtcttgcgt
     7501 ctttgcacct ttaagattag gggcttccca tgagtgcgtt aaataagaaa agttttctta
     7561 cttacctgaa agagggcggt atttatgtcg ttcttttagt tttgctggcg attattattt
     7621 tccaggacac aacattttta agtctgttga acttaagtaa tattctcacc cagtcatcgg
     7681 tgcgtattat tatcgcgctc ggtgtggcag ggttaattgt cacccagggg accgatcttt
     7741 ctgctggtcg tcaggtaggg ctggtggcag tggtggctgc gacattattg cagtccatgg
     7801 ataacgccaa caaagtgttc ccggaaatgg cgacgatgcc gattgcgctg gttattctga
     7861 ttgtctgtgc cattggtgcg gtgatcggtt tgatcaacgg tctgattatc gcttatctca
     7921 acgtgacgcc gttcattacc acgctcggca cgatgatcat cgtctatggc atcaactcgc
     7981 tctattacga ctttgtcggg gcgtcgccaa tttctggttt tgacagtggc ttctctacct
     8041 ttgctcaggg ctttgtcgcg ctggggagtt tccgtctctc ttacatcacc ttctacgcgt
     8101 tgattgcggt ggcgttcgtc tgggtgttgt ggaacaaaac ccgcttcggt aagaacattt
     8161 ttgccattgg cggtaacccg gaagcggcaa aagtatctgg tgtcaacgtc ggcctgaacc
     8221 tgctgatgat ctacgcgttg tctggcgtgt tctatgcctt tggcgggatg ttagaagccg
     8281 gacgtatcgg ctctgccacc aacaacctcg gctttatgta tgagctggat gctatcgcgg
     8341 cgtgcgtggt aggcggtgta tcgttcagcg gcggtgtggg gacggtgatt ggcgtggtga
     8401 ccggggtaat tatttttacc gtcatcaact atggcctgac gtatatcggc gtaaacccat
     8461 actggcagta catcatcaaa ggggcgatta ttatcttcgc cgtagcgctg gattcactga
     8521 aatacgcgcg taagaagtga tgtgattgcg gatattgccg gatgcgctgc atccggcaat
     8581 cggtgccaaa tgccggatgc gtctctggtt ttacaacgtt accgggtgtt ctttctcgcc
     8641 tttcttaaac ttcacttccc cgagctcaat acaacccacc gggcagacgt gaccacaaag
     8701 cagacaaccc acacatttct cggtattaca atgcggtgtg cgggttttct cgctccattc
     8761 catcgcctgg tgaccgccgt cgtaacagga aatataacag cgtccacagc caacacattt
     8821 gtcaagattg atacggggat agacaatata actgcggtct aaatcttccg ccgggacaat
     8881 attgttattc gccagaccta ccatctcctg caacgaatca aatccctgat cggcgagata
     8941 atgcgataac ccgctcgcca tatcttctac gatgcgatag ccgtactgca taatgccggt
     9001 ggtgacctgt aacgttgctg cgccgagcaa taaaaactcc gccgcatctt cccaggtttc
     9061 aatgccgccg ataccgctga ttgggaaatc gcgcagttct ggatgggtgc gcatttgctg
     9121 aatgaagcgc agggcgatcg gttttaccgc tttaccggaa tatccggaaa tactcgattt
     9181 accgttaacg atcggcatac cgattttctg attaagatcg atattggtga tggatttaac
     9241 ggtgttaatt gccgcaatgc catctgcgcc gccgcgcttc gccgccagcg ccacttcgca
     9301 catatcaccg atattcggcg tcatcttcgc cagcattggc agcgtggaac cccgtttcac
     9361 tgcccgacaa tatttttcta ccagctccgg gctttgcccg acatcgctac ccatcgcatg
     9421 agaagtcatt tgcggacagg agaagttaca ctcgatcata tccgcgccag cttcttgcac
     9481 caggcgcgcc agctcctccc attgctgctc attttccccc atgatcgaag cgatcaatac
     9541 tttgtccggg taatcttcct tcagccgacg cagggcggtc agattctctt ccaacggatg
     9601 ttcagcaatc tgctccatat ttttgaagcc gataaaaccg gtatcttctt tcaccagatg
     9661 atcaaaacgc ggcgagactt cgttggcgat aaaaaagccg atcgttttaa acaccacacc
     9721 gccccaacct gtgtcgtagg ctttggcaca catctcatag cagttgccta ccggcgaaga
     9781 agagaggcag aacgggttgg gaaagttcac gccgcaaaaa gtaatcgaaa gatctttcgt
     9841 taacatgagc aagctccctc taaatagtga tgaatcgcct cggcggcttc tttcccggtt
     9901 ttcacggcat agaccacggt tttgtcaccc tcaacaatat cgccagcagc aaagacttgc
     9961 ggatcgcggg tctgataatt ttgtgttttg atggtgttac gctgctgctc taactccgca
    10021 aaggcatcca gtctggcatg ctgaccgacg gcgagaataa ttttatctgc cgccatcgtc
    10081 agttcgcctg ataaccgtac atgcttaaac gtgactttat tgccttccac ggctactggc
    10141 gtgaatccat cgatgatcga aacgcccagc tcccgggcgc tggcaaactc tttttcgctt
    10201 gccggaaact catctaactc ttcacgtgct acgcaagtta ccgcctgaca gccgagaact
    10261 ttcagcgtgc tggctacgtc catcgcgaca tcaccgccgc cgataattaa tgcgctttgt
    10321 ggaatgctga tatcgccttg cgcctgtcgt acacgttgca ggaagtcgac ggcaatctca
    10381 acgtcgctat gctcaaacag cggtagcccg gaaccgcttg ataaccccac ggtgaccagt
    10441 accgcgcggt tttccgcttt cagttgctcc agggtgagtg ttttaccgat ttcgttattg
    10501 cacttaatgg tcacgcccat tttttcaata cgggcgatct ctgcatccag cactgattgc
    10561 ggtaaccgga attgcggaat accgttacgc aaccagccac cggggtgcgc ttctttctca
    10621 taaatcgtga cgtcataacc ttggtttgtc agtgtcacac tggcctgcaa tccggcagga
    10681 cctgcgccaa taatcgcgac tttgccgagc gttttagtac cgggctgata aatttccatc
    10741 ccggtttgtt gttcaaaatc agtaacaaaa cgctgtaagc ggccgatatc aatgggagca
    10801 tcaacaccgg cacgggtaca accgctttgg cataatttct ccgtcgggca cactctggca
    10861 caaacggcac cgagggcatt attttcgcga attgtctcgg cagcgccttt aaaattacga
    10921 aagtagattg agcgaataaa tttccccgga tcggtctgtg ccgggcaagc ctgactacag
    10981 ggagcgtcgt gacataataa acagcgagag gcttctttaa tcgccagtaa cggcgtaaaa
    11041 gccggagtga gttcatccag ataattttgt tgcggcatgt gatcttcctt aatgctatga
    11101 gtaattatgg cgaacgctaa ataaatattg cgtgctgata ttaaggctat gcgcgtgggg
    11161 aaatttaaac tgtgaaagga ttcacaaata gctgcggttc agaatgttat atccgcagct
    11221 tattgagtga agtaataatt attctcaata attaacagaa aagagaaaat attaacgata
    11281 gaaaataaga taaacaatca tgccgataga taaaattccg aagccaatat atgccaccat
    11341 ataagcgaag aagagtccgg gattttttgc tcgccagaca tacaccggtt ccggtgcatt
    11401 tttaacccgt ttatcaatcg cgcctgcgcg ccagcctttc catgcttcgc ggaaacaaaa
    11461 tatcgggata aggaaaaaaa cggcaacgac aaaaaactgc tgtacatcca taactacttt
    11521 ccttgtttct tttgtaattc aagtaactgt tcgggtgtaa cggcgggata cccctgcgca
    11581 tcttccggca cctggtgcat agccggaata gggaccagcg gccctaaaaa acgcggttca
    11641 cgtttaaaaa tataaaggtc agccagcgca ccgaaacggg cggcaaattc gcgaatacgt
    11701 actgacagca tatctttcgg tgacggtacg gcataacact gagcctgaat ccccatatgc
    11761 agcgcaataa ataatgctcg ctcacagtgg aaacgttggg tgataataat gaaatcatta
    11821 gtatcgaaaa ctttgcgtgt acgcacgatg gaatccagcg tacgaaagcc tgcgtaatcg
    11881 agaacaatat ctgatgggtc gacaccagca gcgattaaat ctttgcgcat ggtcatcggc
    11941 tcgttataac tttgcaatgc gttatcgccg ctcagtaata gataatttac cttaccgctg
    12001 ttataggcat taatcgctcc ttgaatgcgg tagcgataat actgattaat aacgccggta
    12061 cgataatatt ttgctgtgcc gagcaccacg ccgacctggc ggtaggggag atcctgcaat
    12121 tcgtcgtaga tataaggcgc ggttttccag ctcatccagc gatcgaggcc gagcacagtc
    12181 aacagcagca agccgatcag gactaacagg ctgaggaaca cgcgctttaa catgcaggag
    12241 actcgagcta aggaaaattt ccatcaggct actttacccg ctggttttgc gcaagaaacg
    12301 gcaaactgtt tgccgggaat ttcgacacca ggagagggct ggcaagccgg acaaggcgtt
    12361 cacgccgcat ccggcaccag gcttaagcga gaagcacgcg gtcgatactg tgacagccga
    12421 gagctttcag cgttgcggag gtggcatccc actgaatcaa cggcgcatcg gctttttctg
    12481 ccagaaccgc gcgctggata tccgggtaat cataaccctt gagattcaac agaattaacg
    12541 ccccctgcaa cggcggcaga gtcgggttga atgcggcgtt ttcagcgtag ctgccgctga
    12601 aaatacgacc gtctttacat tccagcgcga caccgcttgg cgacttactg taaggcatgt
    12661 gcgaacggtt tgccgctgca atcgccgcct gagaaagcgc atcaccagtc agcgcatagc
    12721 cgtgatcctg ctcgtccatc agcagcgttt taatctccag atctttcggc ccaaaggcat
    12781 ccggcagata gtcacgcagc gcgtgtgcct cgcggcccgg caaatgaata cgcagatcca
    12841 gaccgctgtt cagttcattc ataaactgac ggcagtgacc acaaggcgta tagttaacgg
    12901 tgatggctgc aagcgctttt tcaccactca accaggcgtg gctgatcgcg ctttgttcgg
    12961 catgaacggt ttgctgcatt gttgcaccaa taaattccat attggcacca aaataccagg
    13021 ttccgctcac accgcgcgca attgcgccaa cattaaaatt cgacaatggc gtacgcgcac
    13081 aggccgccgc cagcggaagt agtgcgaatg ccagcgcgtc ttcgtccagc cccgttgcgc
    13141 tcttcagcga tgagacttgc tccccggtca acaaagcggg gaagtacttg tctgccagaa
    13201 taggttccag tgcagattgc aagttatccg caagttgggc aaaagcggtt tgaaaacgtg
    13261 gatgcatggc gttgcctcat aatcatgtaa tgggataaca gtttacgaac ccgttatggc
    13321 tttatatgtg atctgaatct cattaattat gcaacacgtg aaacttacat caaaaatgcg
    13381 cgacgtatcg caaattttag cccattactg ccagaataat cgggaaaagg aacggtgcga
    13441 tcagcgaagt aattatcccg cataacacca gcgctagcga actaaatgca ccttcctgat
    13501 aatccagctc ggcgcagcgc gccgtaccga gggcgtgcga ggcagtcccc attgccagac
    13561 cgcgcgcagc tttggtacga atacgcatcg cattaagcaa tgtatggcca aataccgcgc
    13621 cgaggatgcc gacgaaaatc acgcaaactg cgctgattgc cggaataccg ccaatactgc
    13681 cgccaaccgc cattgcaata ggcgtggtga ctgatttcgg caggattgac gcggcgattt
    13741 ccggtgaagc gcccatcaac aatgccacgg aagtccccgt caccatcgca accacgctgc
    13801 cgataaaaca gatggtgatg atcgatttcc agcgcgcgcg gatctggtgt agctgctcat
    13861 ataaaggata ggccagcgcg acgaccgccg gttgcagcag atcgtttagc acttcgctac
    13921 ctttaaagta actgtcgtaa gagatgccgg tcagcattaa aaaaggaatg atcaccacca
    13981 ttgctaccag caacgggtta agcaggggaa atttataccg tgccgccagt ttgcgggcgg
    14041 caaaaaagac aatcaacgtc agcggtaatg accaccagat attcgccatc attcttctga
    14101 tcctttctga cctacgactt tacgttcacc gtgtaccagt tgtgaactcc agctcaccac
    14161 cagaaaaacc accagcgtac tgacagcaca ggaaaccact accgggccaa actgcgcgcg
    14221 gagcaaatca aaatattgca tgacgcctac gccaatcggc acaaacaata gcgccatata
    14281 gcgaatcagt atgtagcacc ccggattgac ccatttcgcc ggaagaattt gcaaggccag
    14341 caggacaaac aggatcagca tcccgatgat gctgcccgga atggttaccg gtagcaggga
    14401 agcaatgaaa atgcctgcat acaggcaggc ataaatcagg acgaaagcgc gtaaatattg
    14461 ccagataatg ttcagtgtct tactcataat caacacccca gatgagatgg attcatcata
    14521 caattaattc gtaaaacgtg ctaccgatca cattaagaaa tcagcgctta ttttttcgct
    14581 acccggtatt acgcaatgag cagcgaactc aggcatacat caagcataga gagggattgc
    14641 agtttgcagc aatccctctt tgcagacctt taatccgctg accataggcg ctaacatagc
    14701 gcctcatttt tttgcgggtg atgatatgcg tgtgttactg gcaccgatgg agggagtgct
    14761 tgactctctg gtgcgtgaat tgctgaccga agttaacgac tacgatctgt gcatcaccga
    14821 gtttgtccgc gtggtggatc aactgctgcc ggtaaaagtc tttcatcgca tttgccctga
    14881 gctacaaaac gccagccgga caccatctgg tacgctggtg cgcgtgcagt tgttaggtca
    14941 gttcctacaa tggctggcag agaacgccgc ccgtgcggtg gagttaggtt cctggggcgt
    15001 ggatctcaat tgcggctgcc cgtcgaaaac ggttaacggt agcggcggcg gggcgacgtt
    15061 actcaaagat cctgaactca tctactaggg ggcaaaagcg atgcgtgaag ctgtaccggc
    15121 gcatttgcct gtcagcgtga aagtgcgtct gggctgggac agcggtgaga agaaatttga
    15181 aatcgccgat gcggttcaac aggctggcgc tacggagctg gtggtgcatg ggcggacgaa
    15241 agagcagggt taccgcgcgg agcatattga ctggcaggcg attggcgaga ttcgccagcg
    15301 gctgcgacgc agtgatgatt ggtcgcgggg cgctcaatat tcccaacctg agccgggtgg
    15361 taaaatataa cgaaccgcga atgccgtggc cggaggtggt tgctttgctg caaaaatata
    15421 cccgtctgga aaagcagggc gataccgggt tatatcacgt tgcgcggatt aaacagtggt
    15481 tgagttattt gcgtaaagaa tacgatgaag caacggaatt atttcagcat gttcgggtgt
    15541 tgaataactc ccctgatatt gcaagggcta ttcaggaaat tgatatcgag aaactctaaa
    15601 aaccatagtg atgtttttga caatgtgtta gctctttatc aataggccgc acggttgttt
    15661 ggtggcgtca aaatcgccga ggcgttccct gaaggccggg gcagcccaca tggatgtggg
    15721 ctgagggcgc gttttacagg gatgttacct cgcgcccgac ccggtagccg taagggataa
    15781 gccgagggca ccgcgcagcg gcgattttgt tcgccagagc ccgggggtgc agggggcggc
    15841 ggcgattggt cgccccctgc gcgctccttg cgccagtgat tatatgaagc aatgcacatg
    15901 acaggagcgc aacaggatga gagactgaaa tattctcgtt attttcggat ctgatgacat
    15961 taattaattt taaaatatca tcttaaatac gccagtcacc accagtagcc caatcaggaa
    16021 aataatcaat acagcccaga gtataatttt cattggcgtt cctttatttg tgagtgctga
    16081 ataagtatag gaaaaaacga agccctttgc cgttatccat aaacgattaa gataaatcgg
    16141 aagatatcat ttacttagtc tatatttttc tgggtatgtc gttttattgc tactgatgta
    16201 ttgccacgcg aaactaaaaa atatatcgca acacacggta atctcagcgc accgtttctt
    16261 acctgattat tccccatgaa tcgtgattcc ttttatccag ccatcgcctg ttttccgctg
    16321 ttactgatgc tggccgggtg tgcgcctatg catgaaaccc gccaggcgtt aagccagcaa
    16381 acgcccgctg cacaagttga caccgcatta cccacggcgc tgaaaaatgg ttggccagac
    16441 agccaatggt ggctggagta tcacgataat cagctcactt cattaattaa caatgcacta
    16501 cagaacgcac cggatatgca ggtggctgaa cagcgcattc agctagctga agcccaggcg
    16561 aaggcagttg ccactcagga tggtccgcag atcgactttt cggcggatat ggagcggcaa
    16621 aaaatgtcgg cagaagggct aatggggccg tttgctctga acgatccggc cgcaggtacg
    16681 accggtccgt ggtacaccaa cggaactttt ggcttaacgg cgggctggca tctcgatatc
    16741 tggggaaaga atcgggcgga ggttactgcc cgcctgggta cggttaaagc acgggcggcg
    16801 gaacgcgagc aaacccgcca attgctggct ggcagcgtag cccgcctgta ctgggagtgg
    16861 caaacccagg cggcgttaaa cacggtcttg cagcaaatag aaaaagagca gaacaccatt
    16921 atcgcgaccg atcgccagct atatcagaac gggattactt cttcagttga cggtgtggaa
    16981 accgatatta atgccagcaa aacccggcag cagctcaacg atgtcgcggg gaaaatgaaa
    17041 attattgagg cacggttaaa tgcactcaca aatcaccaga ctaagtcatt gaagcttaaa
    17101 ccggtcgcgt tgccgaaagt ggcaagccag cttcctgatg aactggggta ctccttactg
    17161 gcccggcggg cagatttgca ggcggcgcac tggtacgttg agtcatcgtt aagcactatt
    17221 gatgcggcaa aagcggcatt ttatcctgac atcaacctga tggccttcct gcaacaggat
    17281 gcgttgcact taagcgatct gttccgtcat tccgcgcagc aaatgggcgt tacggcaggc
    17341 ctgacgctac ctattttcga tagtggtcgt cttaacgcca atctcgatat cgcaaaagcc
    17401 gaaagcaact tgtctatcgc cagctacaac aaagcggtgg ttgaagcagt gaatgacgtg
    17461 gcgcgggcag ccagtcaggt tcagacactg gcggagaaaa accagcatca ggcgcaaatt
    17521 gagcgcgatg ccttgcgtgt ggtaggtctt gcgcaggccc gctttaacgc gggcatcatt
    17581 gctggttccc gcgtcagcga agccagaatc ccggcgctgc gtgagcgggc caatggcctg
    17641 ttattgcaag ggcagtggct ggatgcctcc attcaactca ccggtgcgct gggcgggggt
    17701 tacaaacgct gatgatatgc gccttctata cttaacgttt attcagcgtt aaatggagaa
    17761 ctcgatggca taggtcgcga taattaccgc ttccgattcg gggatcggca aagagtgcgc
    17821 gttattactg gcgcagcagg ggtttgatat tggcattacc tggcactcag atgaagaagg
    17881 ggcaaaagat accgcgcgtg aggtggttac ccacggcgta cgtgcggaga tcgtgcaact
    17941 ggatctcggc aatctgccag aaggtgcgca ggcgctggag acactcatcc agcgatttgg
    18001 gcgtattgat gtgctggtga ataatgcggg tgcaatgacc aaagcgccgt ttcttgatat
    18061 ggcttttgat gagtggcgca agatttttac cgttgatgtt gatggtgcat ttttatgctc
    18121 gcaaattgcg gctcgtcaga tggtgaaata agggcagggc ggtcgcatca tcaacattac
    18181 gtcggtacat gaacatacgc cgctgccgga tgccagcgcc tacacagccg ctaaacatgc
    18241 gctcggcggg ttaaccaaaa cgatggcgct ggagttggtc aggcataaga ttttggtgaa
    18301 cgcagtcgcg tctggggcga tagccacgcc aatgaacggc atggatgata gcgacgtgaa
    18361 gcccgacgcg gagccttcga ttcctctacg gcgttttggc gcaacgcatg agattgccag
    18421 cttggtggtg tggctttgtt cggagggcgc aaattacacg acagggcagt cgttgatagt
    18481 ggatggcggc tttatgttgg cgaatccgca gttcaaccca gaatagcgca gtgaggtgcg
    18541 tggatatttc aatgggttag gccagataag acatcagcgt cgcatcaggc atggaacacc
    18601 cggtgccgga tgtggagtga atgcttatct gtggcaactg ctttacgcct gattatccgg
    18661 ctttttcttc ccgcgccgtt tcagccacca gcgcacgccc accaccagaa ccacgaccag
    18721 aatcaaccag acccagtgct tcaaatgctg gtcgagattg tgcaaccacg gcgcaatcac
    18781 ctgaccacca gcgtaaccaa tagtggtaaa aatcaacgcc caggcaaatg cgccgagaat
    18841 attcagcggc agaaagattt tcggcggcag ctggctggca ccaatcagcg tcgggccaat
    18901 cacccgaaag ccatacataa agcgcgtacc aatgacaaac agatacggat ggcgttggat
    18961 aagtttctgc gcccgctcaa ttttatcctg atgtttcgag aaacggcgta acagcttgcc
    19021 gccaaaccgc cgcccgcaca gatagagcac ctggtcgcca atcatgccac caagcgccac
    19081 agaaagtacc accagtggga actttaatag cccctgatgt gccgcaacgc ctcccagcaa
    19141 agtcacggtt tcaccttccg ccaggctacc gataaccagc gcggcataac catattgtga
    19201 gatgagtgta ttgagatcca taggccagtc tgtctccctt tggttgcctg taagcataca
    19261 ccctgtgaat cattcacgct gaaaaaggac atacatcaga cggtgcaaat tacatggatg
    19321 aacaaaaaat aatcacaagc tggcttatac ttgaactgtc aggttcatga actcacaaca
    19381 agggggcggt atgagccatg tctggggact gttttcccat cccgatcgtg aaatgcaggt
    19441 gattaatcgc gaaaacgaaa cgatttctca ccattacacc caccacgttt tgctgatggc
    19501 ggcgattccg gtgatttgcg ccttcattgg cactacacag attggctgga attttggcga
    19561 tggcactatc ctgaagttat cctggtttac cggactggcg ctggctgtct tgttttacgg
    19621 cgttatgctg gctggggttg cggtgatggg gcgggtcatc tggtggatgg cgcgcaatta
    19681 cccgcagcgt ccgtctcttg cgcactgcat ggtcttcgcg ggctacgttg caactccgct
    19741 gtttttaagc ggtctggtgg cgctttatcc actggtctgg ttgtgtgcgc tggtcggcac
    19801 ggtggcgctg ttttacaccg gctacctgct ttaccttggt attccgagct tcctgaatat
    19861 caacaaagag gaaggcctta gcttctccag ttcgacgctg gcgattggcg tgctggtact
    19921 ggaagtgctg ctcgccctaa ccgttatact atggggctac ggttatcgtt tgttctagaa
    19981 agttctttga cgttgcattg ctggcgtaaa tgtcagcaat gcagcattta ctcacgattc
    20041 tcctttgacg atcgcacttt ttgctcgtta tgatggcgtc gctagcctca gtaaatcctt
    20101 actccggcgg tgcgcaaccc gtgcgcgtga accactatct gaatgctcat catgccgaaa
    20161 tttcgagttt ctttatttag cctggccctg atgctggctg tgccttttgc accgcaggcc
    20221 gttgcgaaaa cggcagccgc taccaccgct tcacaaccgg aaattgcctc cggtagcgcg
    20281 atgattgttg atctgaatac caacaaagtg atctattcga accacccgga tctggtgcgt
    20341 ccgattgcgt ctatcagcaa attaatgacc gcgatggttg tgctggatgc acgactgccg
    20401 ctggatgaaa aactaaaagt ggatatcagc cagacgccgg agatgaaagg ggtctattcg
    20461 cgcgtacgac tgaatagcga aatcagccgt aaagatatgc tgttgctggc gctgatgtct
    20521 tcagaaaacc gcgcggcggc aagccttgcg caccattatc ccggtggtta caaagccttt
    20581 attaaggcaa tgaatgcgaa agcgaaatcg ctcggaatga acaacacgcg ctttgttgaa
    20641 cctaccggat tgtcggtgca taacgtttca actgcccgtg acttaaccaa actgctcatt
    20701 gccagcaaac aatatccgtt gatcgggcag ttaagtacta cccgggaaga tatggcaacc
    20761 ttctctaacc cgacgtatac gctgccgttc cgcaatacta atcatctggt gtatcgcgat
    20821 aactggaata ttcagttaac caaaaccggc tttaccaatg cggcgggcca ttgtctggtg
    20881 atgcgtacgg ttatcaataa taaaccggtg gcgctggtag tgatggacgc gtttggcaaa
    20941 tatacccatt ttgccgatgc cagccgcctg cgtacctgga ttgaaaccgg taaagtgatg
    21001 cctgtgccgg cagcagcgtt gagctataaa aaacaaaaag ccgcccaaat ggcggcggcg
    21061 gggcagacgg cacagaacga ttaagtgaat tcggatggcg atactctgcc atccgtaatt
    21121 tttactccac ttcctgccag tttttccgtt tactggtttt accgatcccc ggattcatgc
    21181 tattggtcgg atcgttctcg cgatagaact tctgcaacgt ctccggtgct ttatacaaat
    21241 gaccgacgtt atgctcggca gggtactgcg cgccgcgctg ctgtagcagt tccagcatct
    21301 gttcttttaa cgcatgcaca tccacgcctt ttttcactat gtaatcctga tggaagacat
    21361 agcacataaa atggccgtaa tagagcttgt gcaccagctg gctgtcgatc tccggcggta
    21421 aatgctcata ccactcggtg tcgttacgcc gcagagcgat atccagtgcc agaatgtctt
    21481 cgacttcatc ggaatgcacc gcctgataac gaattgctgc gcccgcagcg gcgaaacggt
    21541 gtaaaaacgc tttgctacct tcctccggcg tacagacaaa gaaatcgccc tcggcctgtt
    21601 tgaaataatc caccagccac gatttggctt caccgacgcc atcgcctgcc atttttaaca
    21661 gcagatgatg ctcgtattta tcgcgccagt ttttcatgcg cggcggtaaa tggctgggga
    21721 acaggtgacc gaatttttgc attgcacggt cagtaaaatg cggacggaag aatttcactt
    21781 tctccagcat cgcatcggtg cgtcccttga gattaaagaa gaacggcatc ttgtcggtgc
    21841 ctaacttatc aatcatcagg aaggtgtctt tgccgtattt ttccgcaata tcgtagatat
    21901 cccggtgcat atattccccg gcaaccggca gattttcgaa gttagccaga atatgacggc
    21961 ggatttcggt cagcacttcc ggctggttgg tgccgatata aaacacctgc tgattttttt
    22021 ccgcttcgaa ggtatcaaga cgtaccgcaa agacggccag cttcccggcg caaccagaag
    22081 attcaaataa ccgatccgga tcggcgttat aacgtgcggg cgtgtcggct tcaatatcac
    22141 gaacgcggtg gacatagtca taatcgtggg cgtgacgacc atcgtgacgc acatcgtcat
    22201 ctttgatgcg atcgtcatcc agcttgctaa ggatctgctc cggcgtttcg cccagatcaa
    22261 tccccagatg gttcaccagc gtcagtttgc catcttcatc tatacgcgcg aataacgaca
    22321 tttcggtata cgccgggcca cgttgcacca gcgagccgcc ggagttgtta caaataccgc
    22381 cgatgaccga tgcgcctata cacgatgatc caatcaccga atgcggttcg cgtcccagcg
    22441 gtttgagggc tttttccagc gaatagagcg tggtgcccgg ataggccagc acctgttcgc
    22501 ccttgccgag aacgtgcagc ttgtcgagac gcagggtgct gatgatcacg atatcgcgat
    22561 catagtcgtt accgtttggc gtcgatcctt cggtcaggcc tgtattggca gcctgcatca
    22621 gaataatttt gtcggcggtg acgcaggctt tcagcacccg ccacaattcc agtagtgagc
    22681 cagggaaaac gacagccagc gcgtcgccct gaccagaacg gaagcccttg cgatagcggg
    22741 ccgtttttgc gggatcggtg agcaggtgtg aatgaccgac cagacgggca agttcattca
    22801 aaaaggcttt attatcagtt gttgtcatgg aagacatttt ccactccttg tggtggcgaa
    22861 aaaatatcac aaactagaat agcgccttgt atgacaattc agcgagtgaa tattttgaaa
    22921 attcagacaa taaactgagc attctttttc tctctccatc atgcttatgg caaactacga
    22981 tttttgcacc attttcgctg ccacgtcggg caacaaagga agaaaaatcc atatgaaatg
    23041 gctatgttca gtaggaatcg cggtgagtct ggtcctgcag ccagcactgg cggatgattt
    23101 attcggtaac catccattaa cgcctgaagc gcgggatgcg ttcgtcaccg atctgctcaa
    23161 gaaaatgacg gttgatgaga aaattggtca gctgcgctta atcagcgtag gcccggataa
    23221 tccgaaagag gcgatccgcg agatgatcaa agacggtcag gttggggcga ttttcaacac
    23281 cgtaacccgt caggatatcc gcgccatgca ggatcaggtg atggaattaa gccgcctgaa
    23341 aattcctctt ttctttgctt acgacgtgct gcacggtcag cgcacggtgt tcccgattag
    23401 cctcggtctg gcctcgtctt ttaacctcga tgcggtgaaa acggtcgggc gtgtctctgc
    23461 ttatgaagcg gcagatgatg gcctgaatat gacctgggca ccgatggtcg atgtctcgcg
    23521 cgatccgcgc tggggacgtg cttccgaagg ttttggcgaa gatacgtatc tcacctcaat
    23581 aatgggtaaa accatggtgg aggcgatgca gggtaaaagc ccggcagatc gttactcggt
    23641 gatgaccagc gtcaaacact ttgccgcata cggcgcggta gaaggcggta aagagtacaa
    23701 caccgtcgat atgagtccgc agcgcctgtt taatgattat atgccgccgt acaaagcggg
    23761 gctggacgca ggcagcggcg cggtgatggt ggcgctgaac tcgctgaacg gcacgccagc
    23821 cacctccgat tcctggctgc tgaaagatgt tctgcgcgac cagtggggct ttaaaggcat
    23881 caccgtttcc gatcacggtg caatcaaaga gctgattaaa catggcacgg cggcagaccc
    23941 ggaagatgcg gtgcgcgtgg cgctgaaatc cggaatcaac atgagtatga gcgacgagta
    24001 ctactcgaag tatctgcctg ggttgattaa atccggcaaa gtgaccatgg cagagctgga
    24061 cgatgctgcc cgccatgtac tgaacgttaa atatgatatg gggttgttta acgacccata
    24121 cagccatttg gggccgaaag agtctgaccc ggtggatacc aatgccgaaa gccgcctgca
    24181 ccgtaaagaa gcgcgtgaag tggcgcgcga aagcctggtg ttgctgaaaa accgtctcga
    24241 aacgttaccg ctgaaaaaat cggccaccat tgcggtggtt gggccactgg cggacagtaa
    24301 acgtgacgtg atgggcagct ggtccgcagc cggtgttgcc gatcaatccg tgaccgtact
    24361 gaccgggatt aaaaattccg tcggtgaaaa cggtaaagtg ctgtatgcca aaggggcgaa
    24421 cgttaccagt gacaaaggca ttatcgattt cctgaatcag tatgaagaag cggtcaaagt
    24481 cgatccgcgt tcgccgcaag agatgattga tgaagcggtg cagacggcga aacaatctga
    24541 tgtggtggtg gctgtagtcg gtgaagcaca ggggatggcg cacgaagcct ccagccggac
    24601 cgatatcact attccgcaaa gccaacgtga cttgattgcg gcgctgaaag ccaccggtaa
    24661 accgctggtg ctggtgctga tgaacgggcg tccgctggcg ctggtgaaag aagatcagca
    24721 ggctgatgcg attctggaaa cctggtttgc ggggactgaa ggcggtaatg caattgccga
    24781 tgtattgttt ggcgattaca acccgtccgg caagctgcca atgtccttcc cgcgttctgt
    24841 cgggcagatc ccggtgtact acagccatct gaacaccggt cgtccgtata atgccgacaa
    24901 gccgaacaaa tacacttcgc gttattttga tgaagctaac ggggcgcttt atccgttcgg
    24961 ctatggtctg agctatacca ctttcaccgt ctctgatgtg aaactttctg cgccgaccat
    25021 gaagcgtgac ggcaaagtga cggccagcgt gcaggtgacg aacaccggta agcgcgaggg
    25081 ggccacggta gtgcagatgt acttgcagga tgtgacggct tccatgagtc gccctgtgaa
    25141 acagctgaaa ggctt
//
LOCUS       DBKEKI010000051        24923 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.051, whole genome shotgun sequence.
ACCESSION   DBKEKI010000051 DBKEKI010000000
VERSION     DBKEKI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.051"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            415..540
                     /gene="timP"
                     /locus_tag="ACUQPX_002064"
     CDS             415..540
                     /gene="timP"
                     /locus_tag="ACUQPX_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HHV9056777.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(572..1348)
                     /gene="sseB"
                     /locus_tag="ACUQPX_002065"
     CDS             complement(572..1348)
                     /gene="sseB"
                     /locus_tag="ACUQPX_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HHV9056778.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGAAAQGE
                     AVVEDSVLDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1490..2773)
                     /gene="pepB"
                     /locus_tag="ACUQPX_002066"
     CDS             complement(1490..2773)
                     /gene="pepB"
                     /locus_tag="ACUQPX_002066"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HHV9056779.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2832..3032)
                     /gene="iscX"
                     /locus_tag="ACUQPX_002067"
     CDS             complement(2832..3032)
                     /gene="iscX"
                     /locus_tag="ACUQPX_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HHV9056780.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3044..3379)
                     /gene="fdx"
                     /locus_tag="ACUQPX_002068"
     CDS             complement(3044..3379)
                     /gene="fdx"
                     /locus_tag="ACUQPX_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HHV9056781.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3381..5231)
                     /gene="hscA"
                     /locus_tag="ACUQPX_002069"
     CDS             complement(3381..5231)
                     /gene="hscA"
                     /locus_tag="ACUQPX_002069"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HHV9056782.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKTLAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRTLKGHSVDEV"
     gene            complement(5248..5763)
                     /gene="hscB"
                     /locus_tag="ACUQPX_002070"
     CDS             complement(5248..5763)
                     /gene="hscB"
                     /locus_tag="ACUQPX_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HHV9056783.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDVAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5859..6182)
                     /gene="iscA"
                     /locus_tag="ACUQPX_002071"
     CDS             complement(5859..6182)
                     /gene="iscA"
                     /locus_tag="ACUQPX_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HHV9056784.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6199..6585)
                     /gene="iscU"
                     /locus_tag="ACUQPX_002072"
     CDS             complement(6199..6585)
                     /gene="iscU"
                     /locus_tag="ACUQPX_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HHV9056785.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6613..7827)
                     /gene="iscS"
                     /locus_tag="ACUQPX_002073"
     CDS             complement(6613..7827)
                     /gene="iscS"
                     /locus_tag="ACUQPX_002073"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HHV9056786.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7939..8427)
                     /gene="iscR"
                     /locus_tag="ACUQPX_002074"
     CDS             complement(7939..8427)
                     /gene="iscR"
                     /locus_tag="ACUQPX_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HHV9056787.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8697..9437)
                     /gene="trmJ"
                     /locus_tag="ACUQPX_002075"
     CDS             complement(8697..9437)
                     /gene="trmJ"
                     /locus_tag="ACUQPX_002075"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HHV9056788.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9556..10359
                     /gene="suhB"
                     /locus_tag="ACUQPX_002076"
     CDS             9556..10359
                     /gene="suhB"
                     /locus_tag="ACUQPX_002076"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HHV9056789.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            10504..11358
                     /locus_tag="ACUQPX_002077"
     CDS             10504..11358
                     /locus_tag="ACUQPX_002077"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHV9056790.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     APWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            11549..12829
                     /gene="csiE"
                     /locus_tag="ACUQPX_002078"
     CDS             11549..12829
                     /gene="csiE"
                     /locus_tag="ACUQPX_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HHV9056791.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLNATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12821..13960)
                     /locus_tag="ACUQPX_002079"
     CDS             complement(12821..13960)
                     /locus_tag="ACUQPX_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HHV9056792.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGCEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14120..15010)
                     /gene="hcaR"
                     /locus_tag="ACUQPX_002080"
     CDS             complement(14120..15010)
                     /gene="hcaR"
                     /locus_tag="ACUQPX_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HHV9056793.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSTEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            15146..16507
                     /gene="hcaE"
                     /locus_tag="ACUQPX_002081"
     CDS             15146..16507
                     /gene="hcaE"
                     /locus_tag="ACUQPX_002081"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HHV9056794.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWVLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16504..17022
                     /gene="hcaF"
                     /locus_tag="ACUQPX_002082"
     CDS             16504..17022
                     /gene="hcaF"
                     /locus_tag="ACUQPX_002082"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HHV9056795.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17022..17342
                     /locus_tag="ACUQPX_002083"
     CDS             17022..17342
                     /locus_tag="ACUQPX_002083"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HHV9056796.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17339..18151
                     /gene="hcaB"
                     /locus_tag="ACUQPX_002084"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             17339..18151
                     /gene="hcaB"
                     /locus_tag="ACUQPX_002084"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HHV9056797.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTCSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALEQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18161..19357
                     /gene="hcaD"
                     /locus_tag="ACUQPX_002085"
     CDS             18161..19357
                     /gene="hcaD"
                     /locus_tag="ACUQPX_002085"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HHV9056798.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIAIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLLPPPWFWSDQYSDNLQFIGDMRG
                     DDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLIDEN
                     IALKSL"
     gene            19454..19876
                     /locus_tag="ACUQPX_002086"
     CDS             19454..19876
                     /locus_tag="ACUQPX_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HHV9056799.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19924..20774)
                     /locus_tag="ACUQPX_002087"
                     /pseudo
     CDS             complement(19924..20774)
                     /locus_tag="ACUQPX_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
     gene            complement(20786..21868)
                     /locus_tag="ACUQPX_002088"
     CDS             complement(20786..21868)
                     /locus_tag="ACUQPX_002088"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HHV9056800.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGLVGMMAKGRGAKRIIGVDMLPER
                     LAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTADWGR
                     VVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAITHRF
                     SLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(21901..22899)
                     /locus_tag="ACUQPX_002089"
     CDS             complement(21901..22899)
                     /locus_tag="ACUQPX_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9056801.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIK
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22924..24435)
                     /locus_tag="ACUQPX_002090"
     CDS             complement(22924..24435)
                     /locus_tag="ACUQPX_002090"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HHV9056802.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     KGASKSTLIRKLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24458..>24923)
                     /locus_tag="ACUQPX_002091"
     CDS             complement(24458..>24923)
                     /locus_tag="ACUQPX_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HHV9056803.1"
                     /translation="VCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIS
                     TPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDI
                     SGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
BASE COUNT         5874 a         6959 c         6222 g         5868 t
ORIGIN      
        1 tcagacaaat ataaacaaat cctgctcaaa attaaaaatt ctaaccggta aaagatatta
       61 cttaaacatg taaatttact ttcctttaaa aaacaaaaaa ccgccaaaat caggcggtta
      121 tttgttgctg gtccggttcg cggcctttcc agcaggttgt attaccgtag taatgcaagc
      181 gcgtctcagc ggagacaata ctcgccagta actctctttt tgtcaagcaa aagagagtta
      241 ttattgttct gttagtgtat tatccactgc ggccctttcc gccgtctcgc aagcgggcgc
      301 tggcttaagg aaaggatgtt ccgcgaccgt tagagcaagc gcgtctcagc gcttgctatc
      361 gcggcaatat cgccagtggt gctgtcgtga tgcggtcttc gcatggaccg cacaatgaag
      421 atacggtgct tctgtatcgt acttattgtt tctggtgcgc tgttttccca agtaaataat
      481 aaccggagtc tctccggcga caatttactg gtggttaaca accttcagag cagcaagtaa
      541 gcccgaatgc cgccctttag ggcggcatat tttagattat ccggttttgt ttaaagtcac
      601 gcaaaaaacc accccagcga cgttcataga atggcgcaat atgttcggta ataaagtggc
      661 taattccttt ttcccctttt ttcacctgac aaatatcgat tggttcatcg ccaggtaatg
      721 tatcggtcgc tacacttccc gtcgcctgaa taatttcttc gatatcacca tcggcttcaa
      781 tgccaataag taaattaggc tgtgcctctt cgttctcttt aattgaacaa ataaaagcac
      841 gcttcaccgg cttaatggtt ttaaataagg tggtgagtga atcaatcatt tgtgctggcg
      901 gctctgcaac ttccgataat atcagcgatt caccgccttc caggatttcc tggctgctca
      961 gcggatttcc ctcttcacca atcaacaaac tgatttcacg cggcataaat tctttaccgg
     1021 tcggcagttt ggcattaagg aagagcgttt cgccaagggt catctcaaac aatgtgcgaa
     1081 cgggcattac gacaaatgcc tgttcgtctt caaccgcctg ttgaagcgct tctaacgagg
     1141 tgaaaaaagg aatgacgctg gtgccgtctt ctttttccca gtgctgtaaa tcaagcacac
     1201 tatcttcaac cacagcctcg ccctgcgccg ccgcaccagg cacccagacg gtggattcca
     1261 gtagagtacg gaaaaaggcc gggcggtgcg ccggttcggt tgctgctttt tccagcaggt
     1321 cttcaagttc gttttttgtt tcggacataa gaaccacaat tcattcaacg ttcggggcgc
     1381 aaatgcctga tgcgctacgc ttatcaggcc tacaagggat tcgcaatttg ttgaatttgc
     1441 agaatttgta ggccggataa ggcgtttacg ccgcatccgg catattagtt tacgccgtta
     1501 acagattagc tatcgtgcgc acaccaagtc ccgtagcgcc cgcagaccac tgttcaaccg
     1561 gcgctttacg gtaagtcgcc gagcagtcga tatgcagcca gccttgctga tagttctcaa
     1621 caaagtgcga caggaagccc gccgccgtgc tcgcgcctgc cggatacgcc gcgcttccgg
     1681 tattgttcag ttcggcaaag ttagacggca gctggctgcg gtggaattcc gccagcggca
     1741 gacgccagaa tggttcgttc tcttgtgagg cactcgccag taaacgaccg gcaagcgcat
     1801 cgtcaaaact gaacagcgcg tgataatcat tacccagcgc agttttcgcc gccccggtga
     1861 gggtcgccgc atcaatgatc atttccggtt tctgcgcact ggcatcaatc agaccatcgg
     1921 caagcaccag acgcccttcg gcatcagtgt tcatcacttc aactttttta ccgttgcgat
     1981 aggtgatgat atcgcccagc ttgaacgcat tgccgctaat caggttatcc gcacagcaga
     2041 ggaacagctt cacgcgcttg ttcagtccgc gcgtaatggc aaatgccagc gccccggtaa
     2101 ccgttgccgc gccgcccatg tccgacttca tcgagtccat aaacgcagtc tgtttgatgc
     2161 tgtagccgcc ggagtcaaaa gtgatacctt tacctaccag gcacgcgtac accggtgctt
     2221 ctttatcgcc agttgggttg taatccagcg ccagcaatac cggagaacgt tctgaaccgc
     2281 gcccgacagt gtgcagcccc atataacctt gctcacgcag atcttcgcct ttggtgatcc
     2341 gataagtcac acgatcgccc gcgacgttgc tgatcagatc aacagcacgc tgtgccagtt
     2401 gcgatggtcc caattcttct gccggtgcgt tgatggtgtc acgcacccag tcgatgatca
     2461 tcaggcggtt atccagttcc tggcgctggg catcgtccag atccggccac tccactttac
     2521 gcgtgccttt cgggcctttg taaccttgcc agaatgccca gcagcgatcc gcatcccagc
     2581 cttcaccgct taactgaaca tgcttgatgc ccagaccgtc aatcttgcgg gccgcacgct
     2641 ggatcagccc cagatcgtct gccccgttca aatgcagggt aatgccgtca ttattaatgc
     2701 tgtaagttgc tttttctccc cagcgcgcgt cggcaggttg ggtagagagg gtaatcttca
     2761 tcgcttctgt cattttagtt atccttattc gtttaacagc aaaacgggct acccccaggc
     2821 agcccgttca ttcactcggc ttcgtccagc cagactaaca aaatcgcttc gaggattttc
     2881 tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat cgcaaatcca ctgatgcata
     2941 tcggtgaatc gaaccgtttt cggatcaaga tcgggatacg catcgtacag tgcttcgcca
     3001 atttcgcggc tatcggtcca cttaagtccc atactaacct ctgttaatgc tcacgcgcat
     3061 ggttgatagt gtaacgcggg atttcgacca ctaaatcttc gtcggtgacg cgcgcctggc
     3121 agcttaaacg gctttccggc tccagtcccc aggctttgtc cagcatgtcg tcttcctgct
     3181 ctgagctttc cggcagtgag tcaaaacctt cacgaacgat gcagtggcag gtggtgcaag
     3241 cacaggattt ttcacaggcg tgttcaatct cgataccgtt acgcagcgct gcgtcgagaa
     3301 tggtttcacc gctattagct tccagaacag cgccatcagg gcagaggtcc tgatgaggca
     3361 aaataacaat ctttggcata ttaaacctcg tccacggaat ggcctttcag cgtacgacga
     3421 accgactggt ccatgcggcg agcggcgaaa tcctgggttt gtttgtctac gtttttaatc
     3481 gcttgttcga tggcgtcaac atcatcgccc tgcgccactt cactcaggtg agcggcagca
     3541 ttgtcaatga cctgacgctc tgcggcgctt aacagcgcgg catcagcagc cagcgcgccg
     3601 tgcagacttt ccagcacacg cgccgcttct actttttgtt ctgccagcat acgggctttt
     3661 acgtcctgct cggcatagct cattgagtct ttgatcatcg aagcgatttc gctatcggtc
     3721 agaccgtaag acggtttgac ctgaatagac gcctcaacgc cggtggattt ctccatcgcc
     3781 gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca cgcgaatatg cgcaccgcca
     3841 gccggtagcg ccggaatacc acgcagcgca aaacgcgcca gtgagcggca gtcctgcacc
     3901 agttcgcgct caccctgcat tacatggata gacatcgccg tctgaccatc tttaaaggtg
     3961 gtgaaatcct gagcgcgggc caccggaata gtggtattac gcggaatcac tttctccacc
     4021 aggccgccca tcgtttcgag gcccagcgac agtgggatca catcaagcag cagcatttcg
     4081 ctgtctggct tgttacccac cagaatatcc gcctgaatcg ccgcgccaat agcgacgact
     4141 ttatccgggt cgatggaagt cagcggtgga cgaccgaaaa attcgcctac ccgttcacgc
     4201 accagcggca cgcgagtaga accgcccacc atcaccactt ccagcacttc gtcagcttct
     4261 acacccgcgt ctttcagcgc gcgacgacaa gccagtaagg ttcgttttac cagtggcgcg
     4321 atcagttcat tgaattgttc acggctgatt tcgccctgcc agcccgcaac gttaacggtc
     4381 acggagtccg catcgctcag cgcgattttg gctgcaatgg cggcatccag cagttcacgc
     4441 tgaacgcggt tatcgctacg atcaggaatg tccgcctgct cgcgaatgta atccgccagc
     4501 agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc cggttgccag cacttcaaac
     4561 acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc cgccaccgag gtcataaaca
     4621 gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga tagccgcagc ggtcggttcg
     4681 ttaagtaagc gcagaacatg caaccccgcc agacgcgccg cgtctttggt gccctgacgc
     4741 tgggcatcgt caaagtacgc cggaacggtg ataactacac catccagctc gcctgccagg
     4801 gcttcagttg cccgcgccgc cagtgttttg aggatgtccg cagaaacgcg caccgggttc
     4861 agtagccccg ccgccgtttc aatcatcggc aggccgtttt cgctggcctg gaattgataa
     4921 ggcagatgcg gatagcgttg ctggatatca gccagcgagc gccccatcag gcgtttaaca
     4981 gaactaatgg tgttggcggt gtcaagcgct gcgttagtac gcgcgtcata acccaccgaa
     5041 tgcccttgct gttgatagtg aacaacagat ggcagcaggt gacggccttc atggtcggct
     5101 aacgtttcgg cctgaccgct gcgcactgtc gccaccagcg agttggttgt gcccaggtca
     5161 ataccagccg ccagacgacg ctgatgcggc gcagcactca aaccaggttc actaatttgt
     5221 aataaggcca tgtttagctt ccagaaatta aaaatcgagc agtttttctt cgagttgttc
     5281 ggcactgctc cgcagtttat cgagaaaacg cagcttacgc acggtatccg ccgccacgtc
     5341 ccacgcctcg ttgtctaact gttcaaccat caactgatgg cgggtatcaa acatcttttt
     5401 cacacgtttg ataaagcttt ccagccgcgc ttcatctttc gcctgttcga tctcgtccag
     5461 ctcttcgcgc agctccaact gttccatcag gaacgcggtg tcgcgcacag tatgctgctc
     5521 gctggcgaga tcaaaaccgt gtaaagaaag caaatattcc gcgcgcatta acggatgacg
     5581 cagcgtttgc caggcctggt taatggttgc agattgctgt acggcggcga gttgttccgc
     5641 ctggcttccg ctggcgaatt tatcaggatg atactgacgt tgtagatcct gaaaacgcag
     5701 gctcagtgcc tgggtatcga gttgataacg ggcaggcaag ccaaagaggg tgaagtaatc
     5761 cataacattc tcagggctgc gatccgccgc gataacggcg aatcaggtaa aacaaacccc
     5821 acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc aaacgtggaa gctttcgccg
     5881 caaccacact catctttaac gttcgggttg gtgaatttaa acccttcatt caggccttct
     5941 tttacgaagt ccagctgcgt accgtccaga aattgcaggc ttttgccatc gaccacgact
     6001 ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg gttcgtcaac aaattccagt
     6061 acataagcca tacctgaaca tccggaggtt ctcacgccca gacgcaggcc aaaccctttg
     6121 ccgcggttag ccagaaaggt atttactcgc gctgctgcac tgtcgctcag tgtaatcgac
     6181 ataaccaaac ctcaactctt attttgcttc acgtttgctt ttatagtccg caatggcggc
     6241 tttgatcgcg tcttctgcca gaatagaaca gtgaattttc accggcggca gttcaagttc
     6301 ttcagcaata tcggtgtttt tgatcgcctg cgcttcgtcg agagacttcc ctttcaccca
     6361 ttcggtgacc agggagctgg aagcgatagc ggaaccgcag ccgtaagttt taaaacgcgc
     6421 gtcttcaatg ataccttcat cgttgacttt aatctgcaac ttcattacgt cgccacaggc
     6481 cggtgcaccc accatgccgc tgccgacgtt ctcgtcgttg ttgtcaaagg aacccacgtt
     6541 acgcggattc tcgtaatggt cgataacttt ttcgctgtaa gccattataa attctcctga
     6601 ttccgatacc gattaatgat gagcccattc gatgctgttc agatccacgc cctgcttgta
     6661 catttcccac agcggagaaa ggtcacgcag acgaccgatg gatttacgaa ctaactcgat
     6721 ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa gagaaacgga tagagctatg
     6781 tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag gacggttcga ggcttgctga
     6841 cgtacaggcg gaacctgaag aaactgcgag gtctttcagc gccataatca gcgactcacc
     6901 ttcaacgtag ttgaagctga cgttgagaat gttcggcgca ccgtgttcca ggtcaccgtt
     6961 caggtaaact tcttcgatat ctttgatgcc gttccacaga cggttacgca ggccgcgcag
     7021 acgttccatc tcggtcgcca tctcttcttt tgcgatgcga taggcttcgc ccattccgac
     7081 gatctggtga acaggcagag tgccggaacg cataccgcgc tcgtgaccgc cgccgtgcat
     7141 ttgcgcttcg atgcgtacac gcggtttacg acgcacatac agcgcaccga tacctttcgg
     7201 gccatagatt ttgtgaccgg agaaagacat caggtcaact ttcaactggc tcaggtcgat
     7261 tggcagtttg cccacgctct gggttgcatc aacgtgatag ataatgccac gagcacggca
     7321 catttcgccg atagccgcga tatcctgcac cacgccgatt tcgttattta cgtgcatgat
     7381 ggaaacaagg atggtgtcgt cacgcatcgc cgcttcgagt tctttcaggt cgataatacc
     7441 gttacgctgc ggtgccaggt aggtgacttc gaaaccttcg cgctccagct gacggcaagt
     7501 atccagtacc gctttgtgtt cggttttgct ggtgatgatg tgcttgcctt ttttctgata
     7561 aaagttggct gcacctttga tcgccaggtt gtcagattcg gttgcaccag aggtaaagac
     7621 gatttcacgc ggatcagcgc cgaccagatc ggcaatctga ttacgggcga tatctaccgc
     7681 ttcttcagcc tgccagccga aacggtgaga acgagaggcc gggttaccaa aggttccgtc
     7741 catcgtcatg aactgcatca ttttctcggc aacacgcggg tccaccggcg tggttgcgga
     7801 gtagtcgaga taaatcggta atttcattgc tctataaact ccgtacatca ctcaatgcaa
     7861 ggaatcaggc taccggctgg atgtacgacc gtgtttacgg agtatttagc actccggcct
     7921 gattctgaat tctttttatt aagcgcgtaa cttaacgtcg atcgcgtctt gtgtgcgggt
     7981 gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc agcacttcct ggttattaac
     8041 cagttcgcct aaagtaatgt tgttgagaaa accggtgaga cggtcgctca aatcacgcca
     8101 cagcgcgtgg gtcaggcatt tatcgccgcc ctggcagccg cctttaccct gacaacgggt
     8161 ggcatctaca gattcgtcaa cggcgctaat cacttcgcca acggcgatgc tgctggcatc
     8221 tttgcctaac agataaccac cgcctggtcc acgtacgctg gaaaccagac catttttacg
     8281 cagacgggaa aacagttgtt ccagataaga aagggaaatt ccctgacgtt cggaaatatc
     8341 agccaacggt accgggcccg cttcagagtt gagcgcaacg tcaagcattg cggtcacggc
     8401 atagcgccct ttagatgtca gtctcatgtc ttacttcacc tcaaactcgc ccctgcccgg
     8461 ggttttttat tgtaaaagtg ggggtattgc atagcagggt caagtctgac attcccgagt
     8521 aaattggtca actatttact tgactgattt agtcgggtat ttaaccttca gtgccatttt
     8581 tttatcgtgg cgtttgtacc tgttgtcgga tgcggtgtga acaccttatc cgacctacga
     8641 ttctgtacat tgtaggcctg ataagacgcg ttaagcgtcg catcaggcaa ccactgttat
     8701 tcggccttgt tacctttatt ctgctgctca atagaagcca gaatcccgcg caggatattc
     8761 aattcctggc tttccgggcg cgcacgggta aacagacggc gcaatttatt catcacctgc
     8821 cccggatggt tttcacggat aaaaccggtt gccagcagcg tttgttccag atgaccataa
     8881 aaacgctcca gatcatcgac cagcggatac ggcgtctctt catgctcgac ctgctcgcca
     8941 ttttcctgag ttgccagcca ggccatacgc acttcatagg caataacctg aaccgccatt
     9001 gccaggttca gcgaactgta ttccgggttt gccgcaatcg cgacatgata atggcatttc
     9061 tgcaactctt cattggtcaa gccgacgcgc tcacgaccaa acaccagcgc caccggggta
     9121 tttgccgctt cagcgacgct tttcagaccg cattcgcgcg ggtcgagcat cggccacggc
     9181 agcgtgcggg agcgtgcgct ggtgcccacc accaagctac aaccggctaa cgcttcgtcg
     9241 agcgtatcga cgatgtgagc attaccaatc acatcgctgg cccctgctgc cagggcaatc
     9301 gcctgggagt cgggtttcac cagtggatta accagccaca gattggttaa tcccattgtt
     9361 ttcatggcac gggcaacaga acccatattg ccggtgtgtg acgtctccac cagcacaatt
     9421 cgaatatttt gcagcattgt ctttcttcat ctaaagatta ttcacgcatc ttatcataaa
     9481 acgaagacag atgccgatct cgctgctata ctctgcgccg ttttcccgtt ctttaacatc
     9541 cagtgagaga gaccgatgca tccgatgctg aacatcgccg tgcgcgcagc gcgcaaggcg
     9601 ggtaatttaa ttgccaaaaa ctatgaaacc ccggacgctg tagaagcgag ccagaaaggc
     9661 agtaacgatt tcgtgaccaa cgtagataaa gctgccgaag cggtgattat cgacacgatt
     9721 cgtaaatctt acccacagca caccatcatc accgaagaaa gcggtgaact tgaaggtact
     9781 gatcaggatg ttcaatgggt tatcgatcca ctggatggta ctaccaactt tatcaaacgt
     9841 ctgccgcact tcgcggtatc tatcgccgtt cgtatcaaag gccgcaccga agttgctgtg
     9901 gtatacgatc ctatgcgtaa cgaactgttc accgccactc gcggtcaggg cgcacagctg
     9961 aacggctacc gtctgcgcgg tagcaccgct cgcgatctcg acggtaccat tctggcgacc
    10021 ggcttcccgt tcaaagcaaa acagtacgcc actacctaca tcaacatcgt cggcaagctg
    10081 ttcaacgaat gtgcagactt ccgtcgtacc ggttctgcgg cgctggatct ggcttacgtc
    10141 gctgcgggtc gtgttgacgg tttctttgaa atcggtctgc gtccgtggga tttcgcggca
    10201 ggcgagctgc tggttcgtga agcgggcggc atcgtcagcg acttcaccgg tggtcataac
    10261 tatatgctga ccggtaacat cgttgctggt aacccgcgtg ttgtgaaagc catactggcg
    10321 aacatgcgtg acgagttaag cgacgctctg aagcgttaat gactcaggcg ggtgatatca
    10381 ctcacccgcc ctcgcctttc aggcgctatt ccgaaatact tcctcaccgc tttactttct
    10441 ttcgtcactc tcccaccatc tttccccgat taatggatga taaagaagtc gcaaggaaag
    10501 gttatggcac tgccagtgaa caaacgcgtt cccaaaattc tgtttattct ctttgttgtt
    10561 gccttctgcg tttatttagt gccgcgcgtt gccatcaact tcttctatta tcctgacgat
    10621 aaaatttacg gtcccgcccc ctggtcggcg gaatccgtcg aatttacggc taaggacggt
    10681 actcgtctgc aaggctggtt tattccttct tcgacgggcc ctgctgacaa cgccatcgca
    10741 accatcattc atgctcacgg caatgccgga aatatgtccg cccactggcc gctggtcagt
    10801 tggttacctg agcgtaattt caacgttttt atgtttgatt atcgcgggtt tggtaaatca
    10861 aaaggcacgc cgtcccaggc cggattgctg gacgatacgc aaagcgccat caatgtggtg
    10921 cgccatcgca gtgatgtaaa cccacaacgg ctggtgctgt tcgggcagag tattggcggg
    10981 gcgaatattc ttgctgttat tggtcagggt gatcgtgaag gcatacgtgc ggtgatcctc
    11041 gactccacat ttgcctctta tgcaaccatc gccaaccaga tgatccccgg cagtggctac
    11101 ttacttgatg agagttacag cggcgaaaat tacatcgcca gcgtcagccc gatcccgctt
    11161 ttactcattc acggtaaagc cgatcacgtt atcccatggc agcacagcga aaagttgtat
    11221 agcctggcaa aagagccaaa acggttgatc ctaatcccgg atggcgaaca cattgatgct
    11281 ttttccgatc gtcacggcaa tgtttatcgc gaacagatgg tggactttat ccttagcgcg
    11341 ttgaatccgc agaactaacc catgatcgct agcacgataa tcattcacaa aaccacctta
    11401 agacatgcta atccactggt cagaacagtt taaaatgaga aaaattctgt gacgcttgcc
    11461 aacatttctg atgattagca ttcccttcgc catttccctg agcaaacttt agctattctt
    11521 atcaattatg cttatgggag atctacagat gatgcctacg cttgctccac catctgtcct
    11581 ttcggctccc cagcgccgct gccagatctt gctgacgctc tttcagccgg ggttgaacgc
    11641 caccatggca accttcagcg agcttaatgg tgtggatgat gatattgcca gtcttgatat
    11701 cagcgaaaca ggacgggaga tcctgcgcta tcatcaactc acactgacga ctggttatga
    11761 cggtagctac cgggttgaag gtacagtgct taaccaacgt ttgtgtttat ttcactggct
    11821 acgacgtggt ttccgtctgt gtccgtcatt tattaccagc caattcaccc ccgccctgaa
    11881 gagtgaactg aagcggcgcg gaattgcgcg taacttttac gacgatacca atctacaagc
    11941 gttagtgaat ctctgctccc gacggctgca aaaacgcttt gaatcgcgcg atattcattt
    12001 cctgtgtctg tatctgcaat attgtttgct gcagcaccac gctggaatta cgcctcagtt
    12061 taatccgctc caacgtcgct gggcggaatc ctgccttgaa tttcaggtag cgcaggaaat
    12121 tggacgccac tggcagcgtc gggcgctcca gcatgtaccg cctgatgagc cactgtttat
    12181 ggcattactt ttttccatgt tgcgggttcc cgatccactg cgggatgcgc atcagcggga
    12241 cagacaattg cgtcagtcta tcaaacgtct ggtaaaccat tttcgtgagc tgggaaatgt
    12301 tcgtttttat gatgaacagg ggttatgtga tcagctttat atccacctcg cccaggcgtt
    12361 aaatcgcagt ttgtttgcca tcggtattga taataccctg ccggaagagt tcgccagact
    12421 gtatccacgc ctggtacgca ccacccgcgc ggcgctggcc ggatttgaaa gtgaatacgg
    12481 cgtccatctt tctgatgagg aaagtggtct ggtcgcggtg attttcggtg cctggctaat
    12541 gcaggaaaac gatctgcatg aaaaacagat tattctactg accgggaatg atagcgagcg
    12601 agaagcgcag attgagcagc agttacgcga actaacgtta ctgccgctca acattaagca
    12661 tatgtcggta aaggcatttt tgcagacagg cgctccgcgt ggcgcggcac tgattattgc
    12721 gccttatacc atgccgttac cgctcttttc accaccgctg atctatacgg acctgacgct
    12781 gacaacacat caacaggagc agatccgcaa aatgctcgaa tcagcatgag ggaacaactt
    12841 tcgggcgcaa aaacattgcc ggaagcgcca ccagcgccat tacccagaac acgccgtggc
    12901 ccagatattg atacaggaaa ccggcgaaaa cggtcatgat agcgatactg ccgcccatcg
    12961 cgacggcaga gtaaaccgcc tgtaaacgga tgacttcgca accctggcga gcagcaatat
    13021 aacgcatggc ggccaggtgg cagaccgtga aggtgccgca atgcagaatt tgcaccacta
    13081 tcaaccacgg caacgccgta gttgctccca taatgcccca gcgcactacg ccgcaaatcg
    13141 ccgagatcaa cagcatatcg cgagcactac aacggcggaa aagtttatta ctcagcgcaa
    13201 agataatgac ttccgccacc acgcccagcg accacaaata ccccaccgcc gaggccgagt
    13261 agccagctgc ctgccagtaa atggcgctaa aaccgtaata ggccgcatgt gccccctgca
    13321 ataaacaaac gcaggccaga aagcgccagt tctggcgaac cagcgccaac cacgcagacc
    13381 aaccggtgct ctcctgctgg cggcttgccc cttgtggctg aatcgtcgga cggatgagaa
    13441 agccgagcag catggatgcc acgcccaacg tcaacagcac gaggatcacc cgataatcaa
    13501 acatagtgac cagtttgccc gtcagcgccg agccaatgac aaacgccacc gagccccaca
    13561 gtcgcacttt gccgtaatca agcgggaact gcttttgcca cgtattcgcc agtgcatcgg
    13621 tcaacggtac cagcggtgag aaaaagaggt taaagccaat catcaccagc atcaaccacg
    13681 ctacgtgcgc ccccgcccag aaggcgacag caaagagaag tggcagcagt gccagcacgc
    13741 gcaaggcgga aatcaggcgg gaaggatcgc tgacgcgggg cgcgatgagc aaactcccga
    13801 gaaaacgggc aaccagacct gcccccaata acaggccgat ggtttctggc gttaaaccaa
    13861 tccctttaag ccagacgctc cagaaaggta aaaaaatgcc gtaactaaaa aagtatgtga
    13921 aatagccgag cgccaaccag cgcgtggatt gcaaaaccat gagtccctcc cgtcaggagg
    13981 cgttagtctg gggttaatcg aatgttggtg caagttgaaa aataagaata ttcgagcctg
    14041 cttcccgatg tctgtaacgc cagttcacaa gcactaaaaa actgcgatcc gcgacgcatg
    14101 ttgcaaaaat aatgcgtaat tatgccgtta cgcttgccaa acgttcctgc acaatggcga
    14161 tgaaatcgcg taatgccggc ttcatctccc ctttcttcca cgccatcagt aaagcaatgg
    14221 aaggcacatt tccggcaatg ggacgaaaaa caacctgccc ggtattaaaa ttattcatat
    14281 aaccgggtat caaagtgacg cccagcccca tgcccaccag attcatggtc accagaatat
    14341 tcgttgccac ctggacgata tttggctggc tgttttcttg cgcaaaccac gctttaacga
    14401 tcggcgcaag cgaaccggaa tacgccggat cggtactgac gaaattcacg ccatccagtt
    14461 gcgcggcggt gatctctttt tcatgtgcta aagggtgatc aaccggtaac accaccacta
    14521 atggttcgtc aaaaagctcc agataatcaa tctccgtgct ataaacagga tggcgcatta
    14581 agccgacgtc gagttcacca cggcgaattt tttcctcctg ttgcgtggtg attaaactca
    14641 ccagctcaat caaggtgtct ggctgtctga gacgaaacat cggtaatact tttggcaata
    14701 aattcacttc cgccgatggc acaaagccaa tggttaattg tctgtcttcc tgaacaattt
    14761 tccgcgcccg taatttggca ttttccgctt gttcgaggat tgccagcgcg tcctggagaa
    14821 aacattctcc cgccgccgtc aacgcgactt tgcgcttatc cctcaccagt aacggaacac
    14881 cgacacagtt ttcaagatcg cggatctggc tgcttaacga aggctgtgag gtatgcagtt
    14941 tttccgccgc acgggtaaag ttcagtgcct gcgccactgc gacgaaatag cgtaaatgcc
    15001 gtagttccat caccttcccc ttgttatcga aaaaacgtct cagctggtag aaattaacta
    15061 tttcacatat tagcaaccaa ccagcaacat ccttatggca caaaaataga aggtcaatac
    15121 atcttatctt tcaggattaa aaaatatgac cacaccctca gatttgaaca tttaccaact
    15181 gattgacacc caaaacggtc gggtcactcc gcgtatttat accgacccgg acatttacca
    15241 actggagctt gagcgtattt tcggtcgttg ctggctattt ctcgcccacg aaagccagat
    15301 cccaaaaccc ggtgatttct ttaacaccta catgggagaa gatgcagttg tcgtggtgcg
    15361 ccagaaagac ggcagcatca aggcgtttct caaccaatgc cgccaccggg ccatgcgtgt
    15421 gagttatgca gattgtggca acactcgcgc ctttacctgc ccgtatcacg gctggtctta
    15481 tggcattaac ggcgagttga tcgatgtacc gctggaacct cgcgcctacc cacaagggtt
    15541 gtgtaaatcc cactgggtgc taaacgaagt tccttgtgtg gagagttata aaggactgat
    15601 ttttggcaac tgggatacca gcgcaccggg cctgcatgat tacctgggtg acattgcctg
    15661 gtatctggat ggcatgctgg atcgtcgcga aggcggcacc gaaattgtcg gcggcgtaca
    15721 aaagtgggtg atcaactgta actggaaatt cccggcagag cagttcgcca gtgaccagta
    15781 tcatgctctg ttcagccatg cttctgccgt tcaggtatta ggggcgaaag atgatggcag
    15841 cgataagcgc ctcggtgatg gacaaaccgc ccgcccggtg tgggaaaccg ccaaagatgc
    15901 gctgcaattt ggtcaggacg gtcacggtag cggtttcttc tttactgaaa aaccggatgc
    15961 taatgtctgg gtcgatggcg cagtttcaag ctattaccgc gaaacctatg ccgaagcaga
    16021 acaacgttta ggtgaagttc gcgccctgcg cctggcgggt cataacaata tattccccac
    16081 gctttcatgg ctaaacggca ctgccacact ccgcgtctgg catccgcgcg gccctgatca
    16141 agttgaagtg tgggcgttct gtattactga caaagccgcc tccgatgaag ttaaagccgc
    16201 ttttgaaaac agcgccactc gtgcttttgg tcctgctggt tttctcgagc aggatgactc
    16261 ggagaactgg tgtgaaatcc agaaattgct taaaggccac cgcgcccgca acagcaaact
    16321 gtgtctggaa atggggcttg gtcaggaaaa gcgccgcgac gacggcattc ctggcattac
    16381 taactatatc ttttcagaaa ctgccgctcg tggaatgtac caacgctggg ccgatcttct
    16441 gagtagcgaa agctggcagg aagtgctcga taaaaccgcc gcttaccagc aggaggtgat
    16501 gaaatgagtg cgcaagtttc actggagtta catcaccgca ttagccagtt tctctttcac
    16561 gaagccagtc tgctggatga ctggaaattt cgtgactggt tggcacagct cgacgaagag
    16621 attcgttaca ccatgcgcac cacggttaac gcgcaaacac gcgaccgtcg caaaggcgtc
    16681 cagccgccga cgacctggat tttcaatgac accaaagacc agctggagcg gcgaatcgcc
    16741 cgtctggaaa cgggcatggc ctgggcagaa gagccgccgt cacgcactcg tcacttaatc
    16801 agcaactgcc aggtaagcga aaccgacatc ccaaacgtat ttgccgtgag ggtgaattat
    16861 ctactttatc gggcacaaaa agagcgcgat gaaacattct atgttggaac gcgtttcgac
    16921 aaagttcgcc gtctggaaga tgacaactgg cgcttgctgg aacgggatat cgtcctagat
    16981 caagcggtga tcacttccca taacctgagt gtactgttct gatgaatcga atttatgcgt
    17041 gtcccgttgc ggatgtgccg gagggtgagg ctctccggat agatacctcg cccgtcatcg
    17101 ccttgttcaa cgtgggcggc gagttttatg ccattaacga tcgttgcagc catggtaatg
    17161 cgtcgatgtc agaagggtat ctggaagatg acgccacggt ggagtgcccg ctacacgccg
    17221 ccagtttttg cctgaaaacg gggaaagcgt tatgcctgcc cgccaccgat ccgctcacca
    17281 cttatccagt acacgttgaa ggtggtgata ttttcatcga cttaccggag gcgcagccat
    17341 gagcgatctg cataacgagt ccatttttat taccggcggc ggatcgggat tagggctggc
    17401 gctggtcgag cgatttatcg aagaaggcgc gcaggttgcc acgctggaac tgtcggcggc
    17461 aaaagtcgcc agtctgcgtc agcgatttgg cgaacatatt ctggcggtgg aaggtaacgt
    17521 gacctgttat gccgattatc aacgcgcggt cgatcagatc ctgacttgtt ccggcaagct
    17581 ggattgtttt atcggcaatg caggcatctg ggatcacaat gcctcactgg ttaatactcc
    17641 cgcagagacg ctcgaaaccg gcttccacga gctgtttaac gtcaacgtac tcggttacct
    17701 gctgggcgca aaagcctgcg ctccggcgtt aatcgccagt gaaggcagca tgattttcac
    17761 actgtcaaat gccgcctggt atcctggcgg cggtggcccg ctgtacaccg ccagtaaaca
    17821 tgccgcaacc ggacttattc gccaactggc ttatgaactg gcaccgaaag tgcgggtgaa
    17881 tggcgtcggc ccgtgtggta tggccagcga cctgcgcggc ccacaggcgc tcgagcaaag
    17941 tgaaacctcg ataatgcagt ctctgacgcc ggagaaaatt gccgccattt taccgctgca
    18001 atttttcccg caaccggcgg attttacggg gccgtatgtg atgttggcat cgcggcgcaa
    18061 taatcgcgca ttaagcggtg tgatgatcaa cgctgatgcg ggtttagcga ttcgcggcat
    18121 tcgccacgta gcggctgggc tggatcttta aggaagcacg atgaaagaaa aaacgatcat
    18181 tattgtcggt ggcgggcaag cggcggcaat ggctgcggcc tcgctacgcc agcaagggtt
    18241 caccggtgag ctgcatctgt tttccgatga gcgacatctt ccttatgaac gacctccact
    18301 ctcgaagtcc atgttgctgg aagattcccc gcagttacag caggtgttac ccgctaactg
    18361 gtggcaggaa aacaatgttc atctgcattc cggtgtaacc atcaaaacgc tgggtcgcga
    18421 cacacgagag ttagtgttaa ccaacggcga aagctggcac tgggatcagc tttttatagc
    18481 aaccggcgcg gcagctcgac cgctgccgtt gcttgatgca ctgggagaac gctgctttac
    18541 cctacgccat gccggtgatg ccgccagact gcgagaagtt ctgcagcccg aacggtcagt
    18601 cgtgattgtc ggtgccggaa ctattggtct ggaactggct gccagcgcca cgcagcgcag
    18661 atgtaaggtg acagtgattg aactggcggc aaccgtcatg ggccgtaatg caccaccgcc
    18721 cgtgcaacgc tatcttttac agcgccatca gcaggctggt gtgcgcattc tgctcaataa
    18781 tgccattgaa catgtggtcg atggtgaaaa agtagaactg acgctgcaaa gtggggaaac
    18841 gcttcaggct gatgtggtga tttacggtat tggtatcagc gccaacgagc aactggctcg
    18901 cgaggccaac cttgatactg ccaatggcat tgccattgat gaggcttgcc gcacctgcga
    18961 tcccgcgatc tttgccggtg gcgatgtggc aatcactcgt cttgataatg gtgcactaca
    19021 ccgctgcgaa agctgggaaa acgccaataa ccaggcgcaa attgccgctg ccgcaatgtt
    19081 agggctaccg ctactgccgc cgccgtggtt ctggagcgat cagtacagtg ataacttaca
    19141 gtttattggc gatatgcgtg gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa
    19201 ggcgatttgg tttaatctgc aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg
    19261 gcgtgagatt cgcccaattc gcaaatggat ccagagcggc aaaacgtttg atgcgaaact
    19321 gctgatagat gagaacatcg cgcttaaatc actgtaacca ggataattag cgaatatctc
    19381 aatgcctggg gcgtggcgag gtgcaagagt gtgtattacg tttaaatcac attatcttgc
    19441 aaagggattg gttatgaaca cactacgtta ttttgatttt ggagctgccc gccccgtttt
    19501 gttattaatt gcccgtatcg ccgtggtctt aattttcatt atttttggtt ttcccaaaat
    19561 gatgggcttt gacggtacgg tccaatatat ggcctcgttg ggcgcgccaa tgccgatgct
    19621 ggcagcgatt attgcggtag ttatggaagt gcccgccgcg atattaatcg tgcttggctt
    19681 tttcacccgt ccgctggcgg tgctgtttat tttctacacg ctgggtacgg cggtgattgg
    19741 tcaccattac tgggatatga ccggcgatgc ggttgggcca aatatgatta atttctggaa
    19801 gaatgtcagt atcgctggcg cgttcttgct attggcaatt accgggccgg gggcaatttc
    19861 tctcgatcgg cgttaggaaa gatgccggat gcggcgtgaa cgccttatcc ggcattttat
    19921 aaattacagc cactctaccc gcaacgacat ctctgaggtt gttgattccc ctggtgctag
    19981 cgcaatgaga tcaccccctt ccgggcgatg atgatcatcc ggcgcatgac tcatcggttc
    20041 cagacagaag aaatcaaacg catatccttt atcaaacgca gggtcggaaa caaagatgaa
    20101 ataacacggt gcaggtggcg tcgtttccat gatgatggca taaccttcct gcggctgctc
    20161 gatgcgggcc tgaccattcc atccggcaaa accattgttc acccactgac gcggcaacgg
    20221 cgcgagctgg ttaaaatcta gttcctgcgg tagctgctcg caaaactcac cagccagtaa
    20281 ccgctcgcct gcgcctgaat ccgcgtttgt ggtgacaacg gaaaataagg atgccagccg
    20341 gtaccaaatg gcagcgtctc tgccccttga ttggtgacag agagcgtcac cgtcagcgta
    20401 tccgccgtta aatgaaacgc ctgacttacc cgatagtgat agacaccgct gcgatgttca
    20461 tacaccagac acaaactatc atcgctgtgc gagacacatt gccactcgcc cagccagcca
    20521 tcgccgtgta gatagtgcgc atcccactca acattcggtt gcagttgata ctcacgcccc
    20581 tgccagacaa aacggttgcc gctcacccgg tttgcaaatg gcaccagcgg aaaacatgag
    20641 gcatcggttg ccacaccgct tttgttacca ggacgtaaga gcggcgtcgt atcgcgccag
    20701 aagccttcga ttacgccgcc ctggtcagaa acgtccagct ttagcgaccc gtgggataag
    20761 gtatagatgg tcatccgcta actccttaat ccgggaagtt aatcacaact ttcccgcatt
    20821 tgccgctcgc catcagcaca taggcatcac ctgcctgttc tagcgagaag cgatgggtaa
    20881 tggcgttacg cggccacagc ttccagtcag tcaaatcgtg ggcgcatttt tccatatgga
    20941 acagactggt cacccaggag ccgataatcc gccgttgatg gtgcatcaga tcagcgctga
    21001 cctcgaattc cacttttccg gtttcaccaa tgtaaaccac ccgcccccag tcagcagtgg
    21061 attgcagtgc cagcaagcga cctgcggcat taccggaaca atcgagcgca acatccgcgc
    21121 caccgtgggt aagttcagca attatctgcg gcagaccttc ggtggttgct aaatagccgt
    21181 gatccatcac ccctaactgt tttgccatcg ccagacgttc cggcagcata tcaacgccga
    21241 tgatccgttt tgcaccgcga cctttcgcca tcatgccgac cagccccaga ccgaccacca
    21301 gcacgttatc gctgccggaa acttcgccgc gcaaaatccc ttcataggcc gtgccaacgc
    21361 cgcaactgat aaacgcacca tcttcgtagc tcagcgcatc cggcaggagg atcagatctt
    21421 tttcttccgc cagcagatat tcggcatgac cgccgtcacg ctgccagccg taagccgctt
    21481 ttcctttgcc agtacaagag ataggaaagc cgcggcggca gttcgggcaa aaaccacagc
    21541 cagaaatgtg atacaccagc acgcggtcgc cctctttaaa atggcggcag ccttgcccca
    21601 tcgccacaat ctgcccgcac ggttcatgac cgttgataaa tccctggtat aacggtttat
    21661 cgggtgccgc cgccgtggca cggtgttgat gatagatata gtggacatcg cttccgcaaa
    21721 tcccggagga tttcattttg atcagtacct ggttaatccc cggcgtcggc accgcaactt
    21781 cccgcagatc gacggttgaa tttcctggta aataagctgc cagcatcgtt ttcataaatc
    21841 cctcattaac aatacgatta attttcatcc ctgccgcacc cgcgccaggg gcgttaaggt
    21901 ttagcgtttc gctttactgc ttcgctgggt cagcaagata ttcgccagca ccgccaccac
    21961 gatgatgacg ccgcgtacca cctgctggaa aaaggagtta ataccgagca gcaccagacc
    22021 gttaccgatt agcgtaatca ccagcacacc aagcaatgta ccgaacaggg aaccgcgacc
    22081 gccggaaagt gccgtaccgc cgaccacgac cgcggcgatg acgtcaaact ccagaccgtt
    22141 tgcggcacct gcgttaccgg aaccgaggcg tgccgccaac aaaatgccgg tcaccgccgc
    22201 taataatccc gaaagggtaa agataagaat gcgcactcga cgaactttaa taccgcacaa
    22261 ctgcgccgcc gtggcattac cgccaacggc aaaaaccgag cgcccgaagg cggtttttcg
    22321 gctaatgaac acaaacagcg caaacaacac catcatgatc agcgcggata ctggcacacc
    22381 gagaaattgt ccgcccagcc agtccagcac ctcgttttcg tcaatcggca ctggcagtgc
    22441 gttggtcata aacagcccca ttccgcgcag ggcgctccac agccccagtg tggcaacgaa
    22501 acttggtacg ttaaacacgc cgcgcagcac cccggcgagc gttcccatca gcgcgcctaa
    22561 cagcaacacc agcagacaag caatcgccaa cggaacttca aattgcagca aaaatgccag
    22621 gcacaccgag acaaaagcca ccatcggccc aacgctgaca tcaatttcac cggagataat
    22681 aatcagcgtc atcgcccagg cggcaatccc aatggtggcg gcatcgcgca gcacgttcat
    22741 ctggttattc aatgagataa agccaggcgc gttcagggag aagaccagat aaagaatggc
    22801 aatcaccacc agcaaaccga tctcattaat atggcgactg acaaattgtt tgagcgagac
    22861 gctcttgccc tgcggcaatg gtaatgacga agcagacatc ttcaggttcc tcggtaaaat
    22921 tcatcagtgc acagacagaa tggcggacat cagctcatcc acattgaccg gcgagtgaaa
    22981 ctcctgcgag aacgtgccgt gctgtaataa caggatgcgg tcacacacca gcggcaactc
    23041 ctccacttca ctggagataa acaccacgct ttttccttcg gcagccagct cacggacaat
    23101 acggtaaatc tgctgtttgg cttcgatatc gacgccgcgc gttggctcgt cgagcaacaa
    23161 aatctggctg gcggcataga cccaacgacc gatcaccact ttttgctgat tgccaccaga
    23221 aagcgtgccg atgggtgttt cgctactggc agccttgacc gtcatccgct gcatcacctc
    23281 ttcggtcagg cggcggatgg tggaccattg cagcacaccg ttggtgctga ttttttgccg
    23341 attggtcagc actgtatttt cgtcaacgcc cagccaggga atgatccccg cttctttgcg
    23401 gttttctggc gtatagccaa tgccgcgttt cagcatgtcg ccgtaatcgg ggcgcatgat
    23461 tttctcgccg ttgataacaa tttcgccctg ttcatacgtc tccagcccaa caatcgcctt
    23521 cagcaattca ctgcgccctg cccccagcag gccagcaatg ccaagcactt cgccacgacg
    23581 tagcgtaaag ctgatatcct ccagcttggg cttatggcgt aacgcacgga cttccagtac
    23641 ggcctgatcc acaatttcct gaggggcaac cggcgcaata tcaacgtgat cacgccctag
    23701 cattagcgac acaatatgat gcgtggaggt gttttcgagc atcacatcgc ccgccacctg
    23761 accatcgcgc ataacggtgg cacaggaggc aatgcggcgg atttcttcca tccggtggct
    23821 gacataaatc accgccacgc ccagtgccga catctttttc accgcactga tcaccagttc
    23881 aacttctgca ctggcaagcg agctggtggg ttcatcaaga atgaccacgc gcggctcgcc
    23941 cttcatcacc cgtgcaattt ccaccagttg cttttgcgcc gggcttagcg ttgaaacaag
    24001 ttgttcagga ctaacgtcaa cgcccagcgc ctgtaagcaa cgttgggcat cctgcgccat
    24061 ttgcaggtaa tcaatcatgc cgttgcggcg aggccattga ccaaggcaga ggttttctgc
    24121 caccgtcagc ccttccacca gacttaattc ctgataaacc gcacgaaccc ccagttcagc
    24181 ggcacggcga gtcaacgtag cttcgtcacc ttccagtcgc gtctcgccaa tccagatatc
    24241 accgctatcc gggcgttcgc tgccggtaag ctttcgaatg agagtcgatt tgctcgcgcc
    24301 ttttttacct aacagcgcac gaacttcgcc tttattgagc gtgaagttaa cgttatccag
    24361 cgcaacgacg ccggggtaac gcttatttcc tgccaccact tttgctaccg ggactgcctc
    24421 tgttgccgtg aacataggga cctctgcgaa tcagcgatta gggcagacca tcaacgtgcg
    24481 ttgccagcca ctgtttacca tcttccgttt tggtatagag atcgattggc acctgaatca
    24541 ctttttcacc gtcggcttgt ttattgataa ccttcaatgt ttgcgcgaaa acagcattgc
    24601 ccattttctt accggaaata tccactaccg ctttcagcac ctgattgttt tccagctcct
    24661 gagcaatttc ggttgtcata tccgaaccga aaacagcaat ttttccggcc tgattttgat
    24721 tacgtaccgc ttttaccgcg ccgagtgtcg caccgcccga ctcccccata atggcgttga
    24781 gatccggcgt ggagataatc agtttttcac caacggaaat cgctttatct aaaacagtcc
    24841 cttcctgatt agcgacaatt tgcgcgccgg gaacgcggga ttttaatact tcttcaaatc
    24901 ctttacgtcg ctgcacacaa act
//
LOCUS       DBKEKI010000052        24852 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.052, whole genome shotgun sequence.
ACCESSION   DBKEKI010000052 DBKEKI010000000
VERSION     DBKEKI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.052"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            261..488
                     /gene="feoA"
                     /locus_tag="ACUQPX_002092"
     CDS             261..488
                     /gene="feoA"
                     /locus_tag="ACUQPX_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HHV9056804.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            505..2826
                     /gene="feoB"
                     /locus_tag="ACUQPX_002093"
     CDS             505..2826
                     /gene="feoB"
                     /locus_tag="ACUQPX_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HHV9056805.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2826..3062
                     /gene="feoC"
                     /locus_tag="ACUQPX_002094"
     CDS             2826..3062
                     /gene="feoC"
                     /locus_tag="ACUQPX_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HHV9056806.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3265..4142
                     /gene="rpnA"
                     /locus_tag="ACUQPX_002095"
                     /pseudo
     CDS             3265..4142
                     /gene="rpnA"
                     /locus_tag="ACUQPX_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
     gene            4215..4484
                     /locus_tag="ACUQPX_002096"
     CDS             4215..4484
                     /locus_tag="ACUQPX_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HHV9056807.1"
                     /translation="MQAVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            4478..4843
                     /locus_tag="ACUQPX_002097"
     CDS             4478..4843
                     /locus_tag="ACUQPX_002097"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HHV9056808.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(4844..5632)
                     /gene="bioH"
                     /locus_tag="ACUQPX_002098"
     CDS             complement(4844..5632)
                     /gene="bioH"
                     /locus_tag="ACUQPX_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HHV9056809.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            5670..6353
                     /gene="gntX"
                     /locus_tag="ACUQPX_002099"
     CDS             5670..6353
                     /gene="gntX"
                     /locus_tag="ACUQPX_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HHV9056810.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRTTRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLYCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            6412..6987
                     /gene="nfuA"
                     /locus_tag="ACUQPX_002100"
     CDS             6412..6987
                     /gene="nfuA"
                     /locus_tag="ACUQPX_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HHV9056811.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            7348..8664
                     /gene="gntT"
                     /locus_tag="ACUQPX_002101"
     CDS             7348..8664
                     /gene="gntT"
                     /locus_tag="ACUQPX_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HHV9056812.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(8709..10793)
                     /gene="malQ"
                     /locus_tag="ACUQPX_002102"
     CDS             complement(8709..10793)
                     /gene="malQ"
                     /locus_tag="ACUQPX_002102"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HHV9056813.1"
                     /translation="MESKRLDNAALTAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10803..13196)
                     /gene="malP"
                     /locus_tag="ACUQPX_002103"
     CDS             complement(10803..13196)
                     /gene="malP"
                     /locus_tag="ACUQPX_002103"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HHV9056814.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            13808..16513
                     /gene="malT"
                     /locus_tag="ACUQPX_002104"
     CDS             13808..16513
                     /gene="malT"
                     /locus_tag="ACUQPX_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HHV9056815.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELVAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(16556..17572)
                     /gene="rtcA"
                     /locus_tag="ACUQPX_002105"
     CDS             complement(16556..17572)
                     /gene="rtcA"
                     /locus_tag="ACUQPX_002105"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HHV9056816.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITAQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(17576..18820)
                     /gene="rtcB"
                     /locus_tag="ACUQPX_002106"
     CDS             complement(17576..18820)
                     /gene="rtcB"
                     /locus_tag="ACUQPX_002106"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HHV9056817.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTLGTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLE
                     TLPSRDLAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQ
                     TLAMEEINCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGN
                     EESFCSCSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAV
                     MAAQSDLVEVIYTLRQVVCVKG"
     gene            19009..20607
                     /gene="rtcR"
                     /locus_tag="ACUQPX_002107"
     CDS             19009..20607
                     /gene="rtcR"
                     /locus_tag="ACUQPX_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HHV9056818.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(20589..21347)
                     /locus_tag="ACUQPX_002108"
     CDS             complement(20589..21347)
                     /locus_tag="ACUQPX_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HHV9056819.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(21364..22194)
                     /gene="glpG"
                     /locus_tag="ACUQPX_002109"
     CDS             complement(21364..22194)
                     /gene="glpG"
                     /locus_tag="ACUQPX_002109"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HHV9056820.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(22239..22565)
                     /gene="glpE"
                     /locus_tag="ACUQPX_002110"
     CDS             complement(22239..22565)
                     /gene="glpE"
                     /locus_tag="ACUQPX_002110"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate-cyanide
                     sulfurtransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HHV9056821.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            22755..24260
                     /gene="glpD"
                     /locus_tag="ACUQPX_002111"
     CDS             22755..24260
                     /gene="glpD"
                     /locus_tag="ACUQPX_002111"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HHV9056822.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(24466..24747)
                     /locus_tag="ACUQPX_002112"
     CDS             complement(24466..24747)
                     /locus_tag="ACUQPX_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056823.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIP"
BASE COUNT         5845 a         6767 c         6298 g         5942 t
ORIGIN      
        1 acattagcca gtccgggtaa ttcactattc gaattatatt ttcgctgcga tataaccttg
       61 agccacatca acattgagtc agattattat tcaaaccaac attcgcacac attttaagta
      121 ttgctgatag aaaccattct cattatcatt gtgttgttga ttatttaatc tctcctttgt
      181 tggcaaatca tctggtctca tgtcgcggtc aaacgcccca tgaggtagtt atccagttaa
      241 tgagaaacaa gtaggcacct atgcaataca ctccagatac tgcgtggaaa atcactggct
      301 tttcccgtga aatcagcccg gcatatcgcc aaaaactgct ttctctaggc atgttacctg
      361 gctcctcttt tgatgtggtg cgcgtcgctc cactcggcga ccccattcat atcgaaaccc
      421 gtcgtgtgag cctggtatta cgcaaaaaag atctggcctt attagaagtg gaagcggttt
      481 cctgttaata cggtgataac aacaatgaaa aaattaacca ttggcttaat tggtaatcca
      541 aattctggca agacaacctt atttaaccag ctcactggcg cacgtcagcg tgtaggtaac
      601 tgggctggcg ttaccgtcga acgtaaagaa gggcaattct ccaccaccga tcatcaggtc
      661 acgctggtgg acctgcccgg cacctattct ctgaccacca tctcatcgca gacctcgctc
      721 gatgagcaaa tcgcctgtca ctacattttg agtggcgacg ccgacctgct gattaacgtg
      781 gtggatgcgt ctaaccttga gcgtaacctg tacctgacgc tgcaactgct ggaactcggc
      841 attccctgca tcgtggcgct gaacatgctc gacattgccg agaagcaaaa tattcgtatt
      901 gaaattgatg ctctatcggc acgtcttggc tgcccggtga tcccgctggt ttcaacccgt
      961 ggtcgcggta ttgaagcgct caagctggcg attgatcgct ataaagctaa cgagaatgtg
     1021 gaactggtgc attacgcaca gccgctactc aacgaagcag attcactggc aaaagtgatg
     1081 ccttccgaca tcccgctgaa acaacgtcgc tggctgggcc tgcaaatgct ggaaggcgat
     1141 atctacagcc gcgcctacgc cggtgaagcg tcgcagcatc tggatgccgc cctcgcccgt
     1201 ctgcgtaatg aaatggatga tccggcgctg cacattgccg atgcgcgtta ccagtgcatt
     1261 gctgccatct gtgatgtggt aagcaacacc ctgacggcag aacccagccg tttcaccact
     1321 gcggtagata aaatcgtgct caaccgtttc ctcggtctgc cgattttcct ctttgtgatg
     1381 tacctgatgt tcctgctggc tatcaacatc ggcggggcgt tacagccgct gtttgacgtc
     1441 ggctccgtgg cgctatttgt gcatggtatt caatggattg gctacacgct ccacttcccg
     1501 gactggctga ctatcttcct cgcccagggc ctgggtggcg gtattaacac cgtgctgcca
     1561 ctggtgccgc agatcggcat gatgtacctg ttcctctcct tccttgagga ctccggctat
     1621 atggcgcgtg cagcgtttgt gatggaccgc ctgatgcagg cgctgggctt gccggggaaa
     1681 tcctttgtgc cgctgatcgt cggtttcggt tgtaacgtac cgtcggtaat gggcgcacgt
     1741 acgcttgatg caccgcgtga acgtctgatg accatcatga tggcaccgtt tatgtcctgc
     1801 ggcgcgcgtc tggctatctt cgcagtattc gcggctgcct tcttcgggca gaacggtgcc
     1861 ctagcggttt tctcgctgta tatgctcggt atcgtgatgg cggtgctgac tggcctgatg
     1921 ctcaagtaca ccatcatgcg cggtgaagcg acgccgtttg tcatggagct gccggtctac
     1981 catgtaccac acgttaaaag cctgattatc cagacctggc agcgtctgaa gggcttcgtt
     2041 ctgcgtgctg gtaaagtgat tatcatcgtc agcattttcc tgagcgcttt caacagcttc
     2101 tcgttgagcg ggaaaatcgt cgataacatc aacgactcgg cgctggcttc cgtcagccgg
     2161 gtgataaccc ctgtcttcaa gccgattggc gtacatgaag ataactggca ggcaacggtt
     2221 ggcctgttta cgggcgcaat ggcaaaagaa gtggtggtgg gtacgctcaa caccctctac
     2281 accgcagaaa atattcagga cgaagagttc aatccggcgg aattcaacct cggtgaagag
     2341 ctgttcagtg cggtagatga aacctggcag agcctgaaag acaccttcag ccttagcgta
     2401 ctgatgaacc ccattgaagc tagcaaaggc gacggtgaaa tgggtaccgg ggcgatgggc
     2461 gtgatggatc agaaattcgg tagcgcagct gccgcataca gctacctgat tttcgtcctg
     2521 ctgtatgtac catgtatctc ggtgatggga gccatcgccc gtgaatcaag ccgtggctgg
     2581 atgggcttct ccatcctgtg ggggctgaat atcgcttact cactggcaac attgttctat
     2641 caggtcgcca gctacagtca gcacccaact tacagcctgg tgtgcattct ggcggtaatc
     2701 ctgtttaaca tcgtagttat cggtctgctg cgccgcgcgc gtagccgggt ggatatcgaa
     2761 ctgctggcaa cccgcaagtc ggtaagcagt tgctgcgcag ccagcaccac cggtgattgc
     2821 cattaatggc ttcacttatt caggtacgtg atttgctggc gttacggggc cgtatggaag
     2881 cgacccagat aagccagaca ttgaacactc cgcagccaat gattaacgcc atgctgcaac
     2941 agctggaaag catgggcaaa gccgtgcgga ttcaggaaga acctgacggc tgcctctctg
     3001 gcagttgtaa aagctgcccg gaaggcaaag cctgtctgcg cgagtggtgg gcgctgcgtt
     3061 aaccatactc catcgcctgt tttgaaaagc ccggtatgcg tttgcatccg ggcttttttg
     3121 cgtgcggctt tccataaaaa tgcaattctt gcagcgcaac gtaagttcct ctgaaagcgt
     3181 ctcgcaggga tgaaaactcg ctaatgcaca gatgtggagt ggcgcgtaga gtcgcggcat
     3241 tcaaacaaca ggtgaaggaa cgccatgagt aaaaagcaga gttccacccc acatgatgcg
     3301 ctgttcaaac tctttttacg ccaaccggag acggcacgcg attttcttgc gtttcattta
     3361 ccggcaccca ttcacgcgct ctgtgatatg aaaaccctca agctggagtc gagcagcttt
     3421 attgatgacg atctgcgtga aagctattcc gatgtgctgt ggtcggtgaa aacggaacaa
     3481 ggaccagaat acatctattg tctgattgaa catcaaagcc ctcaaacaaa ctgatcgcat
     3541 ttcgcatgat gcgttacgct attgccgcaa tgcaaaatca ccttgatgct ggatacaaaa
     3601 cgttgccgat ggtggtgcca ttgttgtttt accacggtat tgaaagcccc tatccctatt
     3661 cgctgtgttg gctggattgt ttcgccgatc ccaaactggc aaggcagctt tatgcctccg
     3721 catttccgct gattgatgtc accgtcatgc ctgatgatga aatcatgcag caccgacgta
     3781 tggcgctgct ggagttaatt caaaaacata ttcatcaacg cgatctgatg gggctggtag
     3841 agcaaatggc ctgcttatta agtagtggat acgctaatga cagacaaatc aaagggctgt
     3901 ttaattacat actgcaaact ggcgacgctg tacgttttaa cgattttatc gacggcgttg
     3961 ccgaacgttc accgaaacac aaggagagtt taatgactat tgcggaaaga ttgcggcagg
     4021 agggggaaca atccaaagcc ctgcatatag ccaaaataat gcttgaatcc ggagtccctc
     4081 ttgcagacat catgcgattt accgggctgt cagaagaaga gttggctgcg gcgagtcagt
     4141 agatactcta ccctgcttaa gtgctacttt gtgctatctt gcatgtaaca caaagtagca
     4201 cttagggaac tgatatgcag gcagtgaaat tacggcaaca aggaggagcc atgatcgtta
     4261 ctatcccacg agatcttgcc atcgatcttg gctggtcacc gggcacagag cttaccgtgg
     4321 agaaaaaagg cgatagcgtt aatttgcgcg ccacagagca caaaccgcgt gggcgtctta
     4381 ccgtcgcgca gttgttgagc caaatcgatg aaagcgaaat tacggagcta aatcagtcca
     4441 cagaaggatg ggctgaaggg aaaaaaggta atgaagcatg gtaaaggcac ggacgccaca
     4501 tcgtggtgag atctggtatt ttaaccctga tccggttgcc gggcatgaac ttcaggggcc
     4561 acattattgc attgtggtaa cggacaaaaa actcaacaat gttttaaaag ttgctatgtg
     4621 ctgcccgatt tcaacagggg caaatgcagc acgttccaca ggggtgacgg tgaacgtcct
     4681 cccccgtgat acgcaaaccg gtaacctgca tggcgttgta ctttgtcacc agctaaaagc
     4741 cgtcgatctt attgcccgtg gcgctaaatt tcataccgtt gccgatgaaa aattgattag
     4801 tgaagttatc agtaaactgg tgaatttaat cgacccacaa taattagctc tcacttacca
     4861 ccaacaccct ctgcttcaac gccaccagca ggtgacaaaa ctcgaccgga tgcgaaataa
     4921 acggcgcatg ggccgctttg gcgaagatat atgattcgct gtgaggccaa agtttatcca
     4981 gcatcggcac cactttgcgc ggcaccagac cgtcgagata gccatacaat cgcaaaaacg
     5041 gcatggacac gttttgcagc ggctgacgga gatcgaccgt tttcaggatt tccagcccgc
     5101 cattaagcac gtcaacctcc ggcatcggta acgccagaac ggttttcttc aacgcccgcg
     5161 catcctggcg cgccgtttca gtccccatgg tttgtaacgc caggaaccgc tccactgtac
     5221 gctgaaaatc atcactgagt tgctgctgaa atcccgccag cacgtccggt tttatccccg
     5281 gccactcgtc acgagcacta aaacaaggtg acgacgccac ggtgaccagc gcctgaacac
     5341 gctcgggatg ggttaacgca atctggcttg ccaccagccc gcccagactc cagcctaacc
     5401 aaatggcttt atcaggtgcc tgttgcagca cggcttcggc catatcagca agtgacagcg
     5461 caccaaatcc ccggctacgc ccgaagccgg gcaggtcaac aaggtgcagc gtaaaatgcg
     5521 agctaagttc ctcgtcaatg caacgccaca cttcggcatt cagtccccat ccgtgcagca
     5581 gcacaagatg aacattcccc tgacctttgg tctgccacca gatgttattc atccgctatt
     5641 gttctctttt gacttacaag gatgaacata tgctaacagt accgggatta tgctggctat
     5701 gccgaatgcc actggcgtta ggtcattggg ggatttgttc ggtctgttca cgcaccaccc
     5761 gcacagataa aacgttatgc ccacaatgtg gattacccgc cacacactcc catcttccct
     5821 gcggtcgctg cctgcaaaaa ccgccgccct ggcaaagact ggtcacggtt gccgactatg
     5881 cgccgccgtt aagtccgctt atccaccagc ttaaattttc ccggcgcagt gaaatcgcca
     5941 gcgccctgtc acgtctgtta ctattggaag tcttacacgc tcgtcgcacc accgggttgc
     6001 aattgccgga tcgcatcatc agcgttccgt tatggcagcg gcgtcactgg cgtcggggat
     6061 ttaatcagag cgatttgctg tgtcagccgt tatcacgctg gttgtactgc caatgggata
     6121 gcgaagccgt cacacgtaca cgggccactg cgacccagca ttttctcagt gcccggctgc
     6181 gcaagcgcaa cctgaaaaat gcctttcgtc ttgaattgcc cgtgcaaggt cgccatatgg
     6241 tgattgtgga tgatgtcgtt accaccggaa gtaccgtcgc agagattgcg cagttgcttt
     6301 tacgcaatgg tgcggcgact gtccaggtct ggtgcctttg tcgaaccttg tagagcctcg
     6361 atgatgggcg tattataacc aactaaaata gtcaactatt aggccattac tatgatccgt
     6421 atttccgatg ctgcacaagc gcactttgcc aaactgctgg caaatcagga agaagggaca
     6481 caaatccgcg tatttgtgat taaccctggc acgcctaacg ctgaatgtgg tgtttcttat
     6541 tgtccgccgg atgccgtgga agccaccgac actgccctga aatttgacct gctgaccgcg
     6601 tatgttgatg agttaagcgc accatacctg gaagatgcag agatcgactt tgttaccgac
     6661 cagttgggtt ctcagttaac gctgaaagcc ccgaacgcca aaatgcgtaa agtggcagac
     6721 gatgcaccgc tgatggagcg cgtggagtat atgctgcagt cgcagatcaa cccacagctt
     6781 gctggtcacg gtggtcgcgt ttcgctgatg gaaatcaccg aagacggtta cgccattctg
     6841 caatttggcg gcggctgtaa cggttgttcc atggtcgatg tgacgctgaa agaagggatc
     6901 gagaagcagc tgctgaacga attcccggag ctgaaaggtg tgcgcgatct caccgaacac
     6961 cagcgcggcg aacactccta ctactaagtt atcccctctt ttgtagattg cccgatgcga
     7021 cgctaaagcg tcatttcggg cctacgttga acatgtgcca gacgttggca cagcattgct
     7081 ttggcaaatc cctcccttcg tttccccgcg ataatatgac caacctctca taatttaaat
     7141 ttaccccgct ctggtgattc tcaaacgcca gatgttaccc gtatcattca catgggtacc
     7201 aaacatactc ctgacatctg actacaataa ttagttttag tgggtatcag tcgtggtgcc
     7261 gcaatatctc tgttcccgat tgggataatt agagtttgtc gtcagaaaat tgacgttacc
     7321 cataacaaat gaaaggccag gtaaatcatg ccattagtca ttgttgctat cggtgtaatc
     7381 ttgttgttgc tcctgatgat ccgcttcaaa atgaacggct tcatcgctct cgtcctcgtg
     7441 gcgcttgctg ttggattaat gcaaggaatg ccgctggata aagttattgg ctccatcaaa
     7501 gccggtgtcg gcgggacgct cggtagcctt gccctgatca tgggttttgg cgcaatgctg
     7561 ggcaaaatgc tggcagactg cggtggcgca caacgtatcg ccaccacgct gattgccaaa
     7621 tttggtaaaa agcacatcca gtgggcggtg gtgttaaccg gttttaccgt tggttttgcc
     7681 ctgttctatg aagtgggctt cgtgctgatg ctgccgctgg tgtttaccat cgcggcttcc
     7741 gcgaatattc cattgctgta tgttggtgta ccaatggcgg ctgcactgtc tgtgacccac
     7801 ggcttcctgc caccgcatcc gggcccgact gcgattgcca ccattttcaa tgccgatatg
     7861 ggtaaaaccc tgctgtacgg tactattctg gcaatcccga ccgtgattct cgccggtccg
     7921 gtttacgctc gcgtgttgaa aggtatcgat aagccaatcc cggaaggtct ctacagcgcg
     7981 aaaaccttca gcgaagaaga gatgccgagc tttggcgtca gcgtctggac ctctctggtg
     8041 ccggttgtgc tgatggcgat gcgtgcgatt gccgaaatga tcctgccgaa aggtcacgct
     8101 ttcctgccgg tagcggagtt cctcggtgac ccggtaatgg caacgctgat tgccgtgctg
     8161 attgcgatgt tcacctttgg tctgaaccgt ggtcgttcaa tggatcagat taacgacacg
     8221 ctggtttctt ccatcaaaat cattgcgatg atgctgttga tcatcggtgg tggcggtgcg
     8281 ttcaagcagg tgctggtaga cagcggcgtg gacaaataca ttgcttccat gatgcacgaa
     8341 accaacattt ctccgctgct gatggcctgg tcgattgctg ccgtactgcg tatcgcgctg
     8401 ggttccgcaa ccgttgcggc aatcactgcg ggtggtatcg cggcaccgct gattgcaacg
     8461 acgggtgtta gcccagagct gatggttatt gcggtgggtt ccggtagtgt gattttctct
     8521 catgtgaacg atccgggctt ctggctgttc aaagagtact ttaacctgac tatcggcgag
     8581 accatcaaat cctggtcgat gctggaaacc attatctcgg tgtgcggtct ggtaggctgt
     8641 ctgctgctga atatggtgat ttgataacac tgactgccgg atgcatcgca tccggcatca
     8701 accgcactct acttcttctt cgctgcagct ctgcgccgtc tgtccaaatc cttcagcaac
     8761 ttgttcacgc catcatcggc aaacatcgac tcaagcgttg cggaaagctt gcgtcgccag
     8821 tttttatact ggtaactggt gccaggaata ttcaccggtt cggccatatc cagccagtct
     8881 tccggctgta gtcctaacag agcactgtta ctgtcggcaa tgtagcgctg caaaccacgg
     8941 ttcagcgtcg gcgtcatcga catcaacgat gccttatgcc cggcacgttt tggcagacaa
     9001 ccatatttat gcagtgcatc cagcagccct tgcttcgcca gttcgcgatc ctgatacaga
     9061 ccgcgcagta ccacttcatc cggatacagc cccagggttt tgcccagcgt tagatccccg
     9121 ctctcccagt aaccacgcag cgttggcagg tcatgtgtcg ccgcaaccgc catcgactgc
     9181 tccggatacg ctttcggtgc acggaacgtc ttctcgtggt cgttttcgaa atagagcact
     9241 ttgtaagagt acacaccgct gctgcgcagc ttaccgacaa tctctaccgg tacggtaccg
     9301 agatcttcac caatcaccat acagcgatga cgtttacttt cgagtgccag aatcgagagc
     9361 agatcatcca ccggatagtg aacatacgcg ccctgatctg ccgtctcgcc atacggtatc
     9421 caccacaaac gcagcatcga catcacatgg tcaattcgta atgcgccgca gttttgcata
     9481 ttggcacgca acagctcgat aaacggttca taggcacgcg cggtgatgat atgcgggtcc
     9541 attggcggta atccccagtt ctgccccaac gggccgagga tatccggcgg cgcgccaacc
     9601 gatgctttca ggcaatatag ttcacggtca caccaggttt ccgccccacc ttccgctacg
     9661 ccaaccgcca gatcacgata caagccaatc ggcatttcat agccctggct tatctcccag
     9721 caggcggcaa actggctgta agccagccac tgcaaccaga gataaaaatc gacgtcatca
     9781 cgatgttctt cgcagaactg acgcacttct ggtgaatcca cgttcttata catctctggc
     9841 catgcaggcc agccccagcg catttcgtcc tctttcactt gctgggcatg tagcgcatca
     9901 aaggctgcct gccagaacag gctgtcgccc tgctctgcaa caaactggcg aaacgcggcc
     9961 atctgctcat catcacgttg cgcgaaacct ttccacgcca ttcgtaatgc tgtcattttt
    10021 agggcggtaa ccgtggagta atcgacccag tcggcatcgc gcgcctgttg cagcgtctgt
    10081 tgcgtggtcg gcaactgcca ccaggcctga gcctcttcgc taagatggaa atcttcaacg
    10141 gcgttaacgt cgatataaat cacattcagc caacggcgag aagacgggct gtatgggctg
    10201 gcgctctccg gatttgccgg atagagcgca tgaatcgggt tcaggccaat gaacgacccg
    10261 ccacgttttg ccacatccac cagcatcgct ttgagatcgc caaaatcccc aataccccag
    10321 tttttttccg atcgcagcgt ataaagctga acgcaggcac cccacagctt ttgtttattc
    10381 agcaaagcct gcggttcgta acaacgtttc ggggcgacaa tcacccggca atgcgcgcgc
    10441 tggtcgtcct gggtgagtgt tagcgtgtga taaccttccg gcagcttcgt cggtagattg
    10501 aacgctttgc cccccgttac atggcctttg tactgcgttc cttcttcggt ggtcagctgc
    10561 cagctatatt cgccgctgcc ctccaccacc atcggcattt ttttgccgct ggtataaacc
    10621 atgacattcg ggactggcgt taccgccact ttcgtggcgg tacgttgatg catcgcgtca
    10681 agcaaacgcc gtttggtttc ggcgctaatc gactgcggtt taccgtgggc attgatgtaa
    10741 ttggggctaa tccccgccgt cagcgcggca ttatccagac gtttgctttc catcgagctt
    10801 ccttagcgtt ttgcctgcca gatacgagcc tgataatcgc gaatagagcg atccgagcta
    10861 aacataccgc agcgggcggt attgaggatc gccgcgcgag tccaggcctc ctggtcgcgg
    10921 tacagcacat ccacctgctt ttgtgcctca acataggctg cgaaatccgc catcaccaga
    10981 tacggatcgc cgccctgttt gccgatactg tgcagcatct ggtcgaaggc atgcttatcg
    11041 ccgtcgctgt atttaccgct ttccagctct ttcaatactg cgtccagcac cttatctttc
    11101 ttccgccatt tcaccgggtc gtagcctttg gccagaatag ccttcacttg ttccacggta
    11161 tgaccaaaaa taaagatatt ttcttcaccg actttctcgg cgatttcaac gttcgcacca
    11221 tccagcgtac cgacagtaag cgcaccattg agcgccagtt tcatattgcc ggtaccggaa
    11281 gcttctttac ctgcagtcga aatttgttcg gagatatccg ccgccgggat cagtttttcc
    11341 gccgccgaaa cgcaataatc cggcaggaac accaccttca acttatcgcc aaccagtgga
    11401 tcgttgttga tcacgtcagc cactttgttg atcgcaaaga taatattctt cgccaggtag
    11461 tagcccggtg ccgctttcgc gccgaagagg aagacgcgcg gtacgcgatc agcctgcggg
    11521 ttttcacgaa tttctttgta caacgccaga atatgcagca gattcaggtg ctggcgtttg
    11581 tactcatgca gacgtttgat ctgaatatcg aaaatcgcct gtggattgat ctcaataccg
    11641 gtacgaactt tcacaaactc cgccagacgg actttattcg cctgcttgat ctcgcgatat
    11701 tgctgacgga atttcgcatc atcagcgaat ttttccagat tgatcagctg atcgagatcg
    11761 ttagcccact ctttttgcag tgatttatcc aacagagccg ccagtgcagg gttgcactgt
    11821 ttgatccagc gacgtggggt aataccgttg gtgacgttat ggaatttgtt cggccatagc
    11881 tggtgatatt ccgggaacag atctttcacc accagatccg agtgcagcgc cgcaacaccg
    11941 ttcaccgcga aaccgccaac cacacacagg ttcgccatat gcacttgttt atcgtgcacc
    12001 accgccagtt tggcccacac tttttcatcg cccggccagg ttttctccac cagcgtttta
    12061 aagcgagtat taatttcgtt aataatctgc atatggcgcg gtagtaagcc tttcaccagt
    12121 ttcacatccc agcgttccag cgcttctggc atcagggtat ggttggtgta agcgaaagtt
    12181 ttgctggtga tggcccaggc gtcatcccag ctcatctggt gctcatcgat cagcacgcgc
    12241 agcagttctg gaatcgcgat agttggatgg gtatcgttca gctgaataac ttcgtaatcc
    12301 gccagttcgt gcagtttacg ccccgccaga tgatggcgac gcaaaatatc cgctaccgaa
    12361 caggcacact ggaagtattg ctgcatcagg cgcagctttt taccggcagt atggttgtcg
    12421 tttggataga gaactttggt cagtttttcc gcattgatgc cttgctgttc ggcgcgcagg
    12481 aaatcaccgt cgttaaattt agttagatca aacggatgcg catgcgtcgc ctgccacaga
    12541 cgcagcggct gcgccacgcc attacgatag ccgacaacgg ggagatccca cgcttgaccg
    12601 gtaatggtaa actccggctc ccagcgtccg tctttcatca ctttaccgcc aatccctacc
    12661 tgcacatcca gtgcttcgtt gtggcggaac cacgggtagt tactgcgatg ccagtcatcc
    12721 ggcgcttcaa cctgtttgcc atcgacaaaa gactggcgga acaaaccata ttgatagttc
    12781 agaccgtaac ccgtcgcaga ctgaccgaca gttgccattg agtcgaggaa gcacgccgcc
    12841 agacgtccca gaccaccgtt acccagcgcc gggtcgatct cttcttccag caggtcagtc
    12901 aggttgatgt cataagcctt caacgaatcc tgtacatcct gataccagcc gagattcaac
    12961 aggttgttgc ccgtcagacg accaatcaaa aactccattg agatgtagtt aacatgtcgc
    13021 tgattcgcca ccggtttggc gaatggctga gcacgcagca tttcggccag tgcttcactc
    13081 actgccagcc accactggcg aggagtcatt tcagccgcag aatttaagcc ataacgctgc
    13141 cactgacgtg aaagcgcttc ctgaaattgc ttatcgttaa aaataggttg tgacatagga
    13201 gttccacttt tcttagattt tcaacacaac gttatcgcta gtttgccagg ctcgatgttg
    13261 accttcctca tcctgcgggg gattaggcag ggaggagttg cggggatgag caaggaaatg
    13321 tgatctcgac cacttaaagc tagtgcaaac cacaggatta gcagcaaatc aatgccatac
    13381 agcgcagaaa atctgtatct aagtgcaaaa aatggccgtt gcgtattttc aaaaagcgga
    13441 aggtaactct ataaattaag taaaggagtg aaacagtttc acaagtaaaa tatccagtgt
    13501 gctccatctc attcttaata gatttattaa gatcatcttt ttagatggca ctttcatcag
    13561 gaatgaagaa gaaaccctta cttaaatgaa tctgatgaac ataagggaaa ccagtattca
    13621 cgctggatca gcgtcgtttt aggtgagttg ttaataaaga tttggaattg tgacacagtg
    13681 caaattcaga cacataaaaa aacgtcatcg cttgcattag aaaggtttct ggccgacctt
    13741 ataaccatta attacgaagc gcaaaaaaaa taatatttcc tcattttcca cagtgaagtg
    13801 attaactatg ctgattccgt caaaactaag tcgtccggtt cgactcgacc ataccgtggt
    13861 tcgtgagcgc ctgctggcta aactttccgg cgcgaacaac ttccggctgg cgctgatcac
    13921 gagtcctgcg ggctacggaa agaccaccct catttcccag tgggcggcag gcaaaaacga
    13981 tatcggctgg tactcgctgg atgaaggtga taaccagcaa gagcgtttcg ccagctatct
    14041 cattgccgcc gtgcagcagg caaccaacgg tcactgtgcg atatgtgaga cgatggcgca
    14101 aaaacggcaa tatgccagcc tgacgtcact cttcgcccag cttttcattg agctggcgga
    14161 atggcatagc ccactttatc tggtcatcga tgactatcat ctgatcacta atccagtgat
    14221 ccacgagtca atgcgcttct ttattcgcca tcaaccagaa aatctcaccc tggtggtgtt
    14281 gtcacgcaac cttccgcaac tgggcattgc caatctgcgt gttcgtgacc aactgctgga
    14341 aattggcagt cagcaactgg catttaccca tcaggaagcg aagcagtttt ttgattgccg
    14401 tctgtcatcg ccgattgaag ccgcagaaag cagtcggatt tgcgatgacg tttccggttg
    14461 ggcgacggca ctacagctaa tcgccctctc cgcccggcag aatacccact cagcccataa
    14521 gtcggcacgc cgcctggcgg gaatcaatgc cagccatctt tcggattatc tggtcgatga
    14581 ggttttggat aacgtcgatc tcgcaacgcg ccattttctg ttgaaaagcg ccattttgcg
    14641 ctcaatgaac gatgccctca tcacccgtgt gaccggcgaa gaaaacgggc aaatgcgtct
    14701 cgaagagatt gagcgtcagg ggctgttttt acagcggatg gatgataccg gcgagtggtt
    14761 ctgctatcac ccgctgtttg gcaacttcct gcgccagcgc tgccagtggg aactggtggc
    14821 ggagctgccg gaaatccacc gtgccgccgc agaaagctgg atggcccagg gatttcccag
    14881 cgaagcgatt catcatgcgc tggcggcagg cgatgcgctg atgctgcgcg atattctgct
    14941 taatcacgcc tggagtttgt tcaaccatag cgaactgtcg ctgctggaag agtcgcttaa
    15001 ggccctgccg tgggacagtt tgctggaaaa tccgcagtta gtgttattgc aggcgtggct
    15061 aatgcaaagc caacatcgct atggcgaagt taacaccctg ctagcccgtg ctgaacatga
    15121 aatcaaggac atcagagaag gcaccatgca cgcagaattt aacgctctgc gcgcccaggt
    15181 ggcgattaac gatggtaatc cggatgaagc ggaacggctg gcaaaactgg cactggaaga
    15241 gctgccgccg ggctggttct atagccgcat tgtggcaacc tcggtgctgg gtgaagtgct
    15301 gcactgcaaa ggcgaattga cccgctcact ggcgctaatg cagcaaaccg aacagatggc
    15361 acgccagcac gacgtctggc actacgcctt atggagttta attcagcaaa gtgaaattct
    15421 gtttgcccaa gggttcctgc aaaccgcgtg ggaaacgcag gaaaaagcgt tccagctgat
    15481 caacgagcag catctggaac agctgccaat gcatgagttt ctggtgcgca tccgagcaca
    15541 actgttatgg gcctgggcac ggctggatga agccgaagcg tcggcgcgta gcgggattga
    15601 agtcttgtcg tcttatcagc cacagcaaca gcttcagtgc ctggcaatgt tgattcaatg
    15661 ctcgctggcc cgtggtgatt tagataacgc ccgtagccag ttgaaccgtc tggaaaacct
    15721 gctggggaat ggcaaatatc acagcgactg gatctctaac gccaacaaag tccgggtgat
    15781 ttactggcaa atgaccggcg ataaagccgc cgctgccaac tggttgcgtc atacggctaa
    15841 accggagttt gcgaacaacc acttcctgca aggtcaatgg cgcaacattg cccgcgcaca
    15901 aatcttgctg ggcgagtttg aacctgcgga aattgttctc gaagaactca atgaaaatgc
    15961 ccgcagtctg cggttgatga gcgatctcaa ccgtaacctg ttgctgctta atcaactgta
    16021 ctggcaggcc ggacgtaaaa gtgacgccca gcgcgtgttg ctggacgcat taaaactggc
    16081 gaatcgcacc ggatttatca gtcattttgt catcgaaggc gaagcgatgg cgcaacaact
    16141 gcgccagctg attcaactta atacgctgcc ggaactggaa cagcatcgcg cgcagcgtat
    16201 tctgcgagaa atcaatcaac atcatcggca taaattcgcc catttcgatg agaatttcgt
    16261 tgaacgtctg ctaaatcatc cggaagtgcc agaactaatc cgcaccagcc cgttgaccca
    16321 acgtgaatgg caggtcctgg ggctgatcta ctccggttac agcaatgaac aaattgcggg
    16381 cgaactggaa gtggcggcaa ccaccatcaa gacgcatatc cgcaatctgt accaaaaact
    16441 cggcgtcgcc caccgtcagg ctgccgtgca gcacgcgcag aaactgctga agatgatggg
    16501 ttatggggtg tgagtttagc cggataatgc attggatccg gtttacatct ttacatcatt
    16561 caatactcac ccgcgttacg ccatctgttt ctatcaaacc aaaccgcacc ggcaagaaac
    16621 gctccaccac cgcgatattg gtcagcagat ggcatgaagg atgggcgacc gtaaattctc
    16681 ccgcgcccgc cagcgccatc ggcagtacca gttggtcggc gagatattcc cccaccgccg
    16741 ctgggcttgc caggtagcgt ttcacctctt tcactaactg cgcggctacc acctcggcac
    16801 tgacgcgctt ttcaccgacg acgaaaaagc gttcagtgat attttcactt tcgacttcca
    16861 gcgagacggt attacccggc ccctggtcgc gcggcaggtt atgaatattc tgttcatgca
    16921 gggaaaaact ccccgccagt gtagcgattt cacgctcagc aacatggcgc ggcacaccag
    16981 ctaatagaac ttctccacgc atctgcacaa tgttcccgcg ctcgccaagt tgcaaggtgt
    17041 taaacgatgc cacgggcgag acttccgttg ccaccacacc gcctccggca ggataaaaac
    17101 cgtgacgtaa cagcgtggtt tgctgatgaa ttcctatttt cgccagcagc ggctccagca
    17161 cccggcggat aaaatccgca ggcggcgccg acgggttatc ggtgccgccg ctcacttcaa
    17221 cacgcgaagg tccatcggca aaccacagcg cgggcagcac cgtttgcagc accagcgtac
    17281 aacttccggc gctaccgata gcaaagcggt aatcgccgcc gcgcacggtg ccaggccgga
    17341 agaccagacg ctgcgatccc agctccgcgc cttccaccgt tgccctacaa atttccgcag
    17401 ccgcttttac cgcggtcaga tgctggcgca gcagccccgg tttcgcccgt ccggctcgaa
    17461 tgccagtgat ggtaaatggc tgggcggtta tcatcgacag gctcagcgcc gagcgcagga
    17521 tctgcccgcc gccttcgccc tgtgcgccat ccagcgcaat catccttttc atcatttatc
    17581 cttttacgca caccacctga cgcagggtgt agataacttc caccagatcg ctttgtgccg
    17641 ccatcaccgc atcaatatct ttatacgcca tcgggatttc gtcgatcact tcggcatctt
    17701 tacggcattc cacatgcgcg gtggcacgaa tttggtcttc cacgctgaac agttttttcg
    17761 ctttagttcg gctcattacc cgcccggcac cgtggctgca cgaacagaac gactcttcat
    17821 ttcccagccc acggacgata aagctttttg ctcccatcga accgggaata attccatatt
    17881 gaccagcacg cgcagacacc gcacctttac gcgtcacgta gatctcttca ccaaagtgct
    17941 gttctttttg cacatagttg tggtgacagt tgatctcttc catcgccagc gtttgtggct
    18001 gtctgaccgt tttctgcgta atgctctgca acgccgttac cacattttcc atcatcgcat
    18061 cgcggttaag gctggcgaaa agctgcgccc aggccacggc tcttaggtaa tcatcaaagt
    18121 attccgtacc ttccataaag tacgccagat cacgcgacgg caacgtctca agttggtcct
    18181 gcatctcttt ttgcgccaga tcgataaagt aagtcccgat ggcgttacca attccgcgtg
    18241 aaccggagtg cagcataatc cacacctgct ccgactcatc aaggcagatt tcaataaagt
    18301 ggttacccgt tcccagcgtt cccagcgttc ccagcgttcc caggtgttta tagttgttag
    18361 tgttaaggaa acgcggatat ttttgcgtta accactgata accggcctcc agctcagccc
    18421 atttcgcatc gacgttaaaa ggcgggtttt cccatgcgcc tttatcacgt ttacaacgcc
    18481 cggtagtacg cccgtgtggt acggccgttt caatcgcttg acgcagctcc gccaggtttt
    18541 caggcaggtc tgccgccgtt aacgcggtac gcagcgcgtt cattccacag ccaatatcca
    18601 cgcccaccgc cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac
    18661 ctttacccag gtgtacatca ggcattaccg caatatgttt gaaaataaac ggcatcttcg
    18721 ccgtattaat aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt
    18781 ttaccggggc attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt
    18841 gtttgcggtc ctgataattg caaccgtcgt gccagaaaat taaataagca gattaatttt
    18901 ttaatttatt gtttctaaaa agattatatc tttatgtccg taaacggaga tttcccgcaa
    18961 agccgattta ccgataatga aatatcgcct tttataagga tatctaagat gcgtaaaaca
    19021 gtggcttttg gctttgtcgg taccgtgctg gattatgccg gacgcggcag tcagcgctgg
    19081 tcaaaatggc gtccgtcact ctgtatatgc cagcaagaat cgttggtcat cgatcgactg
    19141 gaattattgc acgatgcccg ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc
    19201 agcgtttcgc cagaaacaga agtggtgggc gttgagattg aactgcataa cccgtgggat
    19261 ttcgaagagg tttacgcctg cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa
    19321 aaagaagact atttaattca catcaccacc ggcacccacg tcgcgcagat ttgctggttt
    19381 ctgctggcgg aagcgcgtta tctacccgcc cggttgatac aatcttcaac tccgcgcaaa
    19441 aaagaacagc cgcgcggccc aggtgaagtg acgattatcg atctcgattt aagccgttat
    19501 aacgccatcg ccagccgctt tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc
    19561 ggtattgcca cgcgtaaccc ccacttcaac cgcatgattg agcagatcga aaaagtggcg
    19621 attaaatccc gcgcgccgat tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg
    19681 gcgcgacgca tcttcgagtt aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa
    19741 gtgaactgcg ccaccctgcg cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa
    19801 ggcgcgttta ccggggcgcg ggaatctcgt gaaggattat tacgcagcgc caacggcgga
    19861 atgttgtttc ttgatgagat tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa
    19921 gctattgaag agaaaacctt ttacccgttt ggcagcgatc gccaggtgag cagtgatttt
    19981 cagcttatcg ccggaacggt gcgcgatttg cgccagctgg ttgccgaagg caaattccgc
    20041 gaagatctgt acgcgcggat caatctctgg accttcaccc tgccgggtct acgccagcgc
    20101 caggaagata ttgaaccgaa cctggattat gaagtggagc gccacgccac actcactggt
    20161 gacagcgtgc gttttaacac cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc
    20221 caggcaacat ggcgcggtaa ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc
    20281 tttgccacta gcggacgcat cactctggac gtggtggaag atgagataaa ccgtctgcgc
    20341 tataactggc aggagagccg cccctccgcg cttacggcgt tgctgggcgc agaggcagaa
    20401 aacatcgatc tcttcgaccg tatgcaactg gaacacgtta tcgctatctg ccgccaggca
    20461 aagtcgcttt ctgctgccgg acgacagctt tttgatgttt cgcgccaggg caaagccagc
    20521 gtcaatgacg cggatcggct acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc
    20581 gtgcaggatc agcacagctc cagttgaata tggtggtccg tcagcacctg catcacgctt
    20641 actggcggcg gggcgtcggt gtagacggca tcaaccatgc tgatgctccc catattgacc
    20701 atcgcattac ggccaaattt tgaatggtca accaccagca taacgtggcg cgagttctca
    20761 ataatggcgc gcttggtacg aacttcgtgg taatcgaact ccagcagcga gccgtcgctg
    20821 tcgatgccgc tgatccccag aatgccgaaa tcaaggcgga actgggagat aaaatcgagc
    20881 gtcgcttcgc caatgatccc gccatcgcgg ctacgtaatt cgccaccggc gagaataatg
    20941 cggaaatctt cttttaccat caacgtgtta gcaacgttga gattgttggt gacaatgcgc
    21001 aaattgctgt gattgagcag cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata
    21061 aacagcgtcg agccattggg gatttgctcc gccactttgc gggcgatgcg ctctttttct
    21121 tcggtctggg tggctttacg atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc
    21181 gcgccgccat gatggcgcag gatcaggttt tgctctgcca gctcgttgag gtcgcggcga
    21241 atagtctgcg ggctgacgga gaagtgctcc accagttcct cggtactgac ataaccctgc
    21301 tgtttaacca gttcgataat accgttgtga cgttgtgttt gtttcattta taaatccctg
    21361 gaattatttt cgttttcgcg cattgagcga atcaacaaaa gccatcgcta aacccacggc
    21421 taatccggcg atgtgtgctc cgttcgccat cgacatccca aacaaatcaa accatccggc
    21481 gacaatccag atcagcgcaa agataattaa cccacgttgc aggtaaatgc cactttgcgg
    21541 atcgcgttcg ccacgtagcc agacgtagcc catcagcgca tacaccacgc cagaaagccc
    21601 gccaaaccac ggcccgctga atttttgctg cacatagccg cttaacaggg cgctgataag
    21661 cgtaatgaca attagcttac cgctaccgag gcgtttttcc accgcgccgc cgagatacca
    21721 ccaccaaagc aggttaaaga ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa
    21781 gtaacgccag aactcaaatt tcagtgttgg atcgaatggc caggccagcc ataacatcac
    21841 ttcctgatcg ccgagaattt gcatggcaat aaacaccacc acgcaggcga tcatcatcac
    21901 ccaggtcacc ggacctgcgc gttcacgcaa ggcagcaaag aaaggataac ggcgataatg
    21961 caggccactg ccggtatggc ctgccagcca gctggccgcc agataacgcg gatctgccgg
    22021 gttttcgaga aaacgcgcca gctccgcccg cacgcgctcg gcctgggact catccgccag
    22081 ccagacatcg ctttggttat gttgttgaat cgtgaggata acaccctgcg tcgccatgta
    22141 atcaacaaac gcctgcgcca cgcgggggtt agcaaaagag gtaatcatca acatcgttgc
    22201 tgtcgcttat tccacacaaa aggggacagt ataaagcgtt acgcgccgta cgccacctct
    22261 gcgggaaact gtcgctgcca ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag
    22321 ccctgttgca gcagatactg cgccgcccct ttgctgctat tgccgtgata acacatcacc
    22381 atcaccggag tgtcaaagtc gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa
    22441 tggaaagcct gcaccgcatg ccccatagcg aaactctgtg gatcgcgaat atcgaccagc
    22501 accgcctctt tttcctgcaa cttctggtgc gcgtcggcaa cgttaataca ttcgaactga
    22561 tccatgcgtc tctctttctt tacaaacaag tgggcaaatt taccgcacag tttacgtcga
    22621 agcggcagat aaacgccata atgttataca tatcactcta aaatgttttt tcaatgttac
    22681 ccaaagcgcg attctttgct aatatgttcg ataacgaaca tttatgagct ttaacgaaag
    22741 tgaatgaggg cagcatggaa accaaagatc tgattgtgat aggtggcggc atcaatggtg
    22801 ctggtatcgc ggcagacgcc gctggacgcg gtttatccgt gctgatgctg gaggcgcagg
    22861 atctcgcttg cgcgacctct tccgccagtt caaaactcat tcacggtggc ctgcgctacc
    22921 ttgagcacta tgaattccgc ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga
    22981 aaatggctcc gcatatcgcc ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc
    23041 gcccggcgtg gatgattcgc attggtctgt ttatgtacga tcatctgggt aaacgcacca
    23101 gcctgccggg atcaactggt ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta
    23161 agcgcggatt cgaatattct gactgttggg tagacgacgc ccgtctggta ctcgccaacg
    23221 cccagatggt ggtgcgtaaa ggcggcgaag tgcttactcg gactcgcgcc acctctgctc
    23281 gccgcgaaaa cggcctgtgg attgtggaag cggaagatat cgataccggc aaaaaatata
    23341 gctggcaagc gcgcggccta gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg
    23401 aggggatgca tctgccttcg ccttatggca ttcgcctgat caaaggcagc catattgtgg
    23461 tgccgcgcgt gcatacccaa aagcaggcct acattctgca aaacgaagat aaacgcattg
    23521 tgttcgtgat cccgtggatg gacgagtttt ccatcatcgg caccaccgat gtggaataca
    23581 aaggtgatcc gaaagcagtg aagattgaag agagtgaaat caattacctg ctgaaagtgt
    23641 ataacgcgca ctttaaaaag cagctaagcc gtgacgatat cgtctggacc tactccggtg
    23701 tgcgtccgct gtgtgatgat gagtccgact cgccgcaagc cattacccgt gattacaccc
    23761 ttgatatcca tgatgaaaac ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga
    23821 ccacctaccg aaaactggca gaacatgcgc tggaaaaact gacgccgtat tatcagggta
    23881 ttggcccggc gtggacgaaa gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg
    23941 acgattacgc cgctcgcctg cgccgccgtt acccgttcct gactgaatcg ctggcacgtc
    24001 actacgcgcg cacttacggc agcaacagcg agctgctgct cggcaatgcg ggaacggtaa
    24061 gcgatctcgg ggaagatttc ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg
    24121 atcacgaatg ggtccgccgc accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt
    24181 ggctgaatgc ggatcaacaa tctcgtgtga gtcagtggct ggtggaatat acgcagcaga
    24241 agttgtcgct ggcgtcgtaa attaacgtaa ggtgatcagg tcagatttca atctggcctg
    24301 agactgatga caaacacaaa actgcctgat gcgctacgct tatcaggcct acgtggttta
    24361 tgcaatatat tgaatttgca tggtcttgta ggccagataa gacgttcacg tcgcatccgg
    24421 catgaacaaa gcgcactttg tcaaaaatct aacctacttt ttaattcagg gaattaccgc
    24481 aaagcccacg ccatcatacc caacgtaaca aaactcaggc acgttcccca ctcgccccga
    24541 gaaaaatagc attaatgcgc ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt
    24601 tggcgtaaag agtaaattgc aaacctcagg aatagcggca agcccagccc ccagcatggc
    24661 aataaccgct gcaaaaaatt gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga
    24721 ggtcaaaatc cattttctat tttgcattat tctttccatt ctttctgaat ggcgaaatta
    24781 tactccccga gtccccttgc cccttctgga cacttttccg aaatgatggc gaaaaaaacg
    24841 ggaccctttg gc
//
LOCUS       DBKEKI010000053        24811 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.053, whole genome shotgun sequence.
ACCESSION   DBKEKI010000053 DBKEKI010000000
VERSION     DBKEKI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.053"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..534
                     /locus_tag="ACUQPX_002113"
     CDS             <1..534
                     /locus_tag="ACUQPX_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HHV9056824.1"
                     /translation="IALAACADKSVDIQTPAPAANTSISATQQPAIQQPNVSGTVWIR
                     QKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFVLPFNPADVQPNAR
                     ILLSAAINVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPVQASGGATTTVPST
                     SPTQVNPSSAVPAPTQY"
     gene            complement(565..876)
                     /locus_tag="ACUQPX_002114"
     CDS             complement(565..876)
                     /locus_tag="ACUQPX_002114"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HHV9056825.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            1039..1135
                     /gene="ffs"
                     /locus_tag="ACUQPX_002115"
     ncRNA           1039..1135
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACUQPX_002115"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1255..1608
                     /locus_tag="ACUQPX_002116"
     CDS             1255..1608
                     /locus_tag="ACUQPX_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HHV9056826.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1650..3200)
                     /gene="pdeB"
                     /locus_tag="ACUQPX_002117"
     CDS             complement(1650..3200)
                     /gene="pdeB"
                     /locus_tag="ACUQPX_002117"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HHV9056827.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PLCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(3364..3834)
                     /locus_tag="ACUQPX_002118"
     CDS             complement(3364..3834)
                     /locus_tag="ACUQPX_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HHV9056828.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3950..4501)
                     /gene="maa"
                     /locus_tag="ACUQPX_002119"
     CDS             complement(3950..4501)
                     /gene="maa"
                     /locus_tag="ACUQPX_002119"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HHV9056829.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4673..4891)
                     /locus_tag="ACUQPX_002120"
     CDS             complement(4673..4891)
                     /locus_tag="ACUQPX_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HHV9056830.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4917..5291)
                     /gene="tomB"
                     /locus_tag="ACUQPX_002121"
     CDS             complement(4917..5291)
                     /gene="tomB"
                     /locus_tag="ACUQPX_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HHV9056831.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     LGNRLFRCFVNATKENPASLSC"
     gene            complement(5837..8986)
                     /gene="acrB"
                     /locus_tag="ACUQPX_002122"
     CDS             complement(5837..8986)
                     /gene="acrB"
                     /locus_tag="ACUQPX_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HHV9056832.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(9009..10202)
                     /gene="acrA"
                     /locus_tag="ACUQPX_002123"
     CDS             complement(9009..10202)
                     /gene="acrA"
                     /locus_tag="ACUQPX_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HHV9056833.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLIQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            10344..10991
                     /gene="acrR"
                     /locus_tag="ACUQPX_002124"
     CDS             10344..10991
                     /gene="acrR"
                     /locus_tag="ACUQPX_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HHV9056834.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            11119..14481
                     /gene="mscK"
                     /locus_tag="ACUQPX_002125"
     CDS             11119..14481
                     /gene="mscK"
                     /locus_tag="ACUQPX_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HHV9056835.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14693..14854)
                     /gene="rsmS"
                     /locus_tag="ACUQPX_002126"
     CDS             complement(14693..14854)
                     /gene="rsmS"
                     /locus_tag="ACUQPX_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HHV9056836.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14868..15395)
                     /gene="priC"
                     /locus_tag="ACUQPX_002127"
     CDS             complement(14868..15395)
                     /gene="priC"
                     /locus_tag="ACUQPX_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HHV9056837.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            15465..15842
                     /locus_tag="ACUQPX_002128"
     CDS             15465..15842
                     /locus_tag="ACUQPX_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HHV9056838.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15995..16546
                     /gene="apt"
                     /locus_tag="ACUQPX_002129"
     CDS             15995..16546
                     /gene="apt"
                     /locus_tag="ACUQPX_002129"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HHV9056839.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16675..18600
                     /gene="dnaX"
                     /locus_tag="ACUQPX_002130"
     CDS             16675..18600
                     /gene="dnaX"
                     /locus_tag="ACUQPX_002130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HHV9056840.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRELLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17933..17997
                     /gene="dnaX"
                     /locus_tag="ACUQPX_002130"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18653..18982
                     /locus_tag="ACUQPX_002131"
     CDS             18653..18982
                     /locus_tag="ACUQPX_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HHV9056841.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     NINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18982..19587
                     /gene="recR"
                     /locus_tag="ACUQPX_002132"
     CDS             18982..19587
                     /gene="recR"
                     /locus_tag="ACUQPX_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HHV9056842.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19697..21571
                     /gene="htpG"
                     /locus_tag="ACUQPX_002133"
     CDS             19697..21571
                     /gene="htpG"
                     /locus_tag="ACUQPX_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HHV9056843.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21752..22396
                     /gene="adk"
                     /locus_tag="ACUQPX_002134"
     CDS             21752..22396
                     /gene="adk"
                     /locus_tag="ACUQPX_002134"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HHV9056844.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            22528..23490
                     /gene="hemH"
                     /locus_tag="ACUQPX_002135"
     CDS             22528..23490
                     /gene="hemH"
                     /locus_tag="ACUQPX_002135"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HHV9056845.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDEALKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(23487..24446)
                     /gene="aes"
                     /locus_tag="ACUQPX_002136"
     CDS             complement(23487..24446)
                     /gene="aes"
                     /locus_tag="ACUQPX_002136"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HHV9056846.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24598..>24811
                     /locus_tag="ACUQPX_002137"
     CDS             24598..>24811
                     /locus_tag="ACUQPX_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="catalyzes the formation of inosine/guanosine
                     monophosphate from inosine or guanosine and ATP; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HHV9056847.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDD"
BASE COUNT         6144 a         6376 c         6404 g         5887 t
ORIGIN      
        1 attgcgttgg cggcttgcgc agataaaagc gtggatattc agacgccagc cccggctgca
       61 aatacgtcta tttcagcaac acaacaacct gctatccagc aaccgaatgt ctccggtacc
      121 gtctggatcc gtcagaaagt cgcactgccg cctgatgctg tgctgaccgt gacactttct
      181 gacgcgtcgt tagccgatgc accgtcaaaa gtgctggcgc agaaagcggt gcgtactgaa
      241 ggtaaacagt caccattcag ctttgttctg ccatttaacc cggcagatgt tcagccgaac
      301 gcgcgtattc tgttgagtgc ggcgattaac gtgaatgaca aactggtatt tatcaccgat
      361 accgttcagc cggtgatcaa ccagggcgga actaaagccg acctgacatt ggtgccggta
      421 cagcaaaccg ccgtgccggt tcaggccagc ggtggcgcaa cgactaccgt accttcgact
      481 tcaccaactc aggtgaatcc gtcttcggca gttcccgctc ctacgcaata ttaagcgacg
      541 ttaaccctct ccgcccggag agggttagta gttccagcga taacgctgca agtcgatttg
      601 cccgcttccc gataccatca caccttctgc cagtaatgcc tgtcgctgac gctgtaaatc
      661 cggcccggtt agcgaaattg tgccatggcg attaaccacc cggtgccagg gtaaggtgct
      721 gccttcaggg agacgcttta acacaccgcc cacctggcgc gcggcgcggg gcgatcccgc
      781 cagtttcgcc acatcaccgt aagttgtgac atagccttcg ggaatagcgg cgacgatttg
      841 ccagacgcgt tggggaaatg aatcttcttt ttccatcttt tcttcctgag gtaatttttc
      901 agcataatct ggaaaaacgc ccgagtgaag tcgcattgcg caagaaacca gcatctggca
      961 cgcaatgggt tgcaattagc cggggcagca gtgataatgc gcctgcgcgt tggttttcaa
     1021 cgctctcaat gggggctctg ttggttctcc cgcaacgcta ctctgtttac caggtcaggt
     1081 ccggaaggaa gcagccaagg cagatgacgc gtgtgccggg atgtagctgg cagggccccc
     1141 acccatttct gcctcccacc gtttcgtcaa aaaatcccag catggctaaa ctttaaccac
     1201 aactgacgtc gcaagaattg tctggctgcg cagtacgctt cggaggtatg tctgatgaag
     1261 tatgttgatg gttttgtggt tgccgttcct gccgataaaa aggatgccta tcgggaaatg
     1321 gccgctaagg ccgcgccatt gtttaaagag tttggcgcgc ttcgtattgt cgaatgctgg
     1381 gccagcgatg taccggatgg caaagtgacc gattttcgta tggcggtgaa agcggaagag
     1441 aatgaagagg ttgtctttag ctggattgaa tacccttcaa aagaggtccg cgacgcagct
     1501 aatcaaaaga tgatgtcgga cccacggatg aaagagttcg gcgagtccat gccgtttgac
     1561 ggcaagcgaa tgatctatgg cggattcgag tcaatcatcg acgaataggt agcgtgacgg
     1621 gctgtgcggc gtgccgtcag cccaaaaatt cacaaatgtt gctcggccca gcgtaagaaa
     1681 tcttctttcg gtaatgcctt gctgtagagc cagccctgac cgtagtgcac gccatgctgg
     1741 cgtagccact cttcttgttt actggtttcg attccctccg ctaccatttt cagtttcagt
     1801 gtttttgcca tttcgatgat atgcggcgtg acatttttat attccagcgc atcaacgaaa
     1861 gatttatcga tcttcagaat gtcgacatcc agatcctgta aataacttaa acttgaatac
     1921 cccgtaccaa aatcatcaag ataaatttca tggcccgcct cccggtagcg agaaattatc
     1981 ggggcgctgg ttttcggatc ggcaaactcg cgttcagtga gttcaagcgc gatctgtctg
     2041 ggattaacct gatagtgatt gatcatttca cgcagcaatt gcgggatttt ttccgaggtg
     2101 agcacggtgg attcaagatt gatcgaaata tgctgctgtg gatgctgacg caagcagtcg
     2161 cccatatctt caaagacgct tcttataatc agtagcgtca atggctcaga aaggcccgtt
     2221 tgctgtgcca gcggaataaa actatctggt gacaaccaac taccgtctgt ctgcggccag
     2281 cgcgccagtg cctcagcacc gacaattttg ccattggcta aggagacaat cggctgatag
     2341 tgcacgcaaa tatcacgatt ttcgatagca tcctgcagcc gatgatgcgg tgactggata
     2401 cggcgcaaaa tacgcagcac aaacatcgct gccagcaggc caatcaccaa cccgagcggt
     2461 aaccaaataa agacttgccg atgccagcct ttctgcaaca ttttcgttga agcccaggtg
     2521 atgatcgaaa tattcatctc cgggaagggc aggatatcgt aaatgatccc attattttcg
     2581 atatgctcac cgggtgtttt ttgtagcctg gtaataattc cctgagcaat ttcatcgctg
     2641 ctggttatga caacgttatg ggcattgcca ataatggcgg catcaatttg ccatgagcta
     2701 tagggaatga catcaataaa ggaagcgggg tcgatcatga cgacataatg tgccgttccc
     2761 atggcgacca tgtaacggat aatgcctaaa tcgttatgcg atgttaacca gacacgataa
     2821 ccatctttcg aaattttacc tggctcggga aaggtatcgg gcggactttc atgctccaga
     2881 gacgaacaca ggggaacgtt gttatcgata taaaccactt cctgaatata gcgataactg
     2941 taagataccc gacgcatttc catgagatgg gcttcgctac aggcagcgcc ttgccatctt
     3001 tccagctcct gcagcgcatc tttcccttgt gtcgccacct tattggctcg aatagcgacg
     3061 cgggaggaat aggtatccag ctcttcaata aacgatgttt ctacctgctg atgggccagc
     3121 cagatgctta agccgacagg cagcaatact gaaagaatca gtactcccga aataaggccg
     3181 accagatgtc gtgttctcac cagcatatcc ttattaacac agtgtaggga aagtatatct
     3241 gattgtggaa aaaggctggc aggagataag gaggtagagc tggtatttca tgaagtgatg
     3301 ggtggaaacg atcattgtca tttgaccagg cgcagagctt tacgcctggt atgtaaccct
     3361 aacttaagat gttgattcgg cgcgagcgag ggtaaaaata tcgtaaaaag acagttcacc
     3421 tttacggacg atcatttttt gcagttgttc cttatgaaca tccgcgacac gcggagagtt
     3481 caagatggct tcaaccagcg cggcaggtac aaagcgcgtg ttgtaccatt cgccgttata
     3541 gcaaacgcgg aaatccagca cgtcgatatc ttcatagcgg tagcgtgggt agacatcgaa
     3601 aaagaagaaa ccattaagca ggataaataa tacaaccagt agccagacag agcctgacag
     3661 cgtttcggac tgcaacatca ccgccagggt ggcaaaaaaa gcaacgtaca taccgataaa
     3721 cagccccgga tgtttacgga taaaactgat actaaagcgg ggtttgttgt cgcgtttttc
     3781 gcgggtattc agagactcaa tcgtttcggt cagcaggcgt tgtatttcgg tcatcgtttg
     3841 ccttcgtggt atctgcaact ttccagggaa caccctattt taacgtggga atagtagagg
     3901 ggaagtaaca gatctaccac aattttgcat aacagttgcg aaaaaccgat tacaattttt
     3961 taattattct ggctggatta ccgcccacga caacgttgtc cgggacacct tttgtgacaa
     4021 ctgcacctga agctaccacg acgttatcac caatggtcac accagggtta atgaccgcgc
     4081 gtccgccaat ccagacgtta ttaccgatgg tgacgggttt ccccagttca gcaccgctat
     4141 tacgtgctac agggtcgatg ggatgtgttg ccgtgtagat atgaacgcct ggtgccaaca
     4201 tacagttatc accgatgcga atagggcaga catcaagcat cacgcaatcg aagttggcga
     4261 aaaaattatt gccgagaaaa atgttatagc cataatcaca gcgaaacgtt ggctcaatat
     4321 aagcctctgt cacctgaccg aataaatcag cgagaatttg ctggcgtaat gtgtgctctt
     4381 ccgccaggga atgattgtat cggtgaataa gctgacgagc gcgcaggcga tcgcgagata
     4441 acgtctcatc tgccgagcga tacaactcac cagcaatcat cttttctttt tctgtgctca
     4501 ttactgacct ccggttgctg aaaatgcggc aacacagtaa gacgaaaagt cagtgaagag
     4561 aacgttctca ttacatctgt gtgataaaga tcacataggg tatgtcttca tggcgaaaaa
     4621 gtataaaatt cttaataaac agccggttat agctccgaaa gcgaattatt tattagcgaa
     4681 taaatttcca tactgaggaa gggatcttgt cgtacagttt attcatggtc aattcggcga
     4741 ggcggtgatc tgcggctgag taaaataccg ccagttcatt atctgataat tcgtatttat
     4801 ttttctcgat aacacgctcc agcgtgtcaa ttgtctggca acgacgtaaa cgcattaaat
     4861 aatcggtttt tgttaaaggt ttttcggaca tacttctacc catggttgta ataattctaa
     4921 caagataaac tcgcaggatt ctctttcgtc gcattgacaa aacaacggaa tagtcgatta
     4981 cctaacttcc gccatttctg aagagcctgc atattaatac cataactgct gaacaacata
     5041 aaggtgtcat ccagatattc gtcgatctgc tcaatgagct tattgtcttc attatactta
     5101 attttgtaat taagtgcgaa ggtcgcaata tgctcaatca gttcattcaa ctggaggttg
     5161 atcgccgagg ttgggtcgtt aacccagcca tgattgcttt cttcaaggtt tgcaagacag
     5221 tcatgataca gggtttcaca gagaaactta agctgtgcga tatcatgtct tttgggtgag
     5281 tattcatcca taacgcgtcc ccttcttagt ggttgaacta acggacacct ttcgggatgg
     5341 aaaaaactta ctgacctgga cttgccctct cgtttgttag cttaactata agccactctt
     5401 tgcaggtttt catcgcatta ttacgaaaaa ttacaattag gaaggaatcg ggacaggact
     5461 cactttttca tactagcact aacccgcagc aggtaaaagc agtttgctcg aactgttcat
     5521 aacatttcag gattatcgcc agatgaaatt ggtgacacta ataccaggat tgctctgaat
     5581 atgacgtaat aaccgaggaa tgaataaaga attaccgcaa ataattaaga atagcctcta
     5641 aatgattatg gattattaaa tctattagcg ctacttaata taaattaact aacaatcagt
     5701 attttttatg aattttatac gtggttaata ctggttttcg tatgagatcc tgagttggtg
     5761 gttcaattac tccttaatgt tcgtaggtta tgcataaaaa aggccgctta cgcggcctta
     5821 gtgattacat gttgtatcaa tgatgatcga cagtatggct gtgctcgata tcttcattct
     5881 tgcggctaaa gcggcggcga accaccacaa agaataccgg aacgaagaag attgccagta
     5941 ccgttgcggt caccatcccg cccattacac cggtacctac tgcgttctgc gcgccggaac
     6001 cagcaccagt actgataacc agcggcataa cgccgaggat aaacgccagc gaggtcatca
     6061 ggatcggacg taaacgcatc cgcaccgcat caagcgtcgc ttcaatcaga cctttacctt
     6121 ctttatccat caagtctttg gcgaattcga cgataagtat cgcgttcttc gccgacaacc
     6181 caatggttgt gagcagtcct acctggaagt aaacgtcatt ggtcaggcca cggaaggtgg
     6241 cagccagcaa cgcaccgata acccccagcg gaacgaccag cataacggag aacggaatcg
     6301 accagctctc gtacagcgcc gccagacaca ggaatacgac aatcaacgaa atcgcgtaca
     6361 gtgaaggtgc ctggttgccg gagagacgtt cctgatagga catccccgtc cagtcatagc
     6421 caacaccggt aggcagtttg ctcgccagtt gttccatcag ctccattgct tcaccggtac
     6481 ttttacccgg tgccgcctgg cctaagattt ccatggatgg caggccgttg taacgttcca
     6541 gacgcggcga accgtactcc caacgagaag aggagaacgc cgagaatggc accatctgac
     6601 catcagcagc acgaacatac cagtcgccga tatcatccgg cagcatacgg tatttcgctt
     6661 ctgacatgac gtaaactttc ttcacacgac cgcggtcgat aaagtcgttc acatagctac
     6721 cgccccatgc agcgcccaga gtggtgttaa tgtcgttgat agaaacaccc agcgcctgcg
     6781 ctttttcctg gtcgatatca atcttaaact gcggggtatc ttccagaccg tttggacgta
     6841 cgctggtcag catatcaggg tgcttcgctg cttctgcaag cagctggtta cgcgcctgag
     6901 tcagtttttc gtgaccaagg ccagcctggt caatcagctc aaagtcaaag ccggttgcag
     6961 tacccagttc cacgattgcg ggcaggttaa aggcgaaaac catcgcatct ttgatttgcg
     7021 agaaagcgcg tgttgcacgc atggtaatcg cttcaacttt gttttcttcg cccggacgat
     7081 cggcccagtc cttcaaggaa acgaacgcaa taccggtatt ctgaccacgt cccgcaaagc
     7141 cgaagccgtt aacggcgaac accgactcaa cgttgttctt ttctttggtc agatagtaat
     7201 gcgttacctc attgagcact ttctgcgtac gttcctgcgt tgcacctgct ggcagctgaa
     7261 ccatggtcat aaatacgccc tggtcctcat ctggcaagaa ggagcttggc agacgcacga
     7321 acagataggc catgccgacc acgatgatca gatacagcac caggtaacgc cccgtactgc
     7381 gcagaatacc gcctacgctg tcggtgtagt ggtgcgtgct cttctcgaac atgcggttaa
     7441 accagccgaa gaagcctttt ttaccttccc cgtgatcgcc tttggcaatc ggtttcagca
     7501 tggtggcaca aagagccgga gtcaggatca acgctaccag taccgacagc gccattgctg
     7561 aaacaatggt aatagagaac tgacgataga tagcaccagt agaaccgcca aagaaggcca
     7621 tcggtacgaa taccgccgac agtaccatcg cgataccgac cagagcgccc tgaatctgcc
     7681 ccatcgactt acgggtagct tcttttggcg gcaaaccttc ttccgccata acacgctcca
     7741 cgttttctac cacaacgata gcgtcatcca ccaacaagcc gatggcgagc accatcccga
     7801 acattgttag cgtgtttatc gagaagccaa aggcggcaag gacggcaaag gtcccgagca
     7861 ataccaccgg tacggcaatg gtcggaatca acgtcgcgcg gaagttctgc aggaacagat
     7921 acataaccag gaacacgagg atgatcgctt cgaccagcgt tttaaccact tcgtgaatag
     7981 agattttcac gaacggcgtg gtgtcgtacg ggtaaacaat tttcagaccc gacgggaaga
     8041 acggttccat cttcgccagt tcagcacgga ttgccgcagc ggtatccagc gcgtttgcac
     8101 cggtcgccag cttgatcccc agaccggaag ccggttggcc gttaaactct gcgatgatgt
     8161 cgtagttctc accacccagc tcaatcttcg ccacatcacg cagcagtacg cgggaaccat
     8221 cctgattcac tttcagcagg attttgccga actcttcagt agaggtcaga cgcgtctgag
     8281 caataataga ggcgttaagc tgttggcctt tcaccggcgg cgtaccaccg agctgacccg
     8341 ccgcaacctg ggcgttctgc gctttgatgg cggtaatgac atcaaccggc gttagctgga
     8401 atttgttcag ctcattcggg ttcatccaga tacgcatcgc gtactgtgaa ccgaacaact
     8461 gaacatcacc cacgcccgac gtacggctga tggcatcttt catattcgcc gctacgtagt
     8521 cagagatatc ctcctgcgtc atggtgccat cggtgttgat aacgccgaca accatcagga
     8581 agctgctgga tgatttctca acgctcaccc cttgctgctg aacttcttgc ggcagcaacg
     8641 gcatcgccag ctgcagtttg ttctgcacct gaacctgcgc gatatccgca tcagtaccag
     8701 actcaaaggt cagggtgatc tgcacggtac ccgtggagtc actgttagag gacatgtaca
     8761 tcaggttatc gataccgttc atattctgtt cgataacctg tgtcaccgtg tcctgcactg
     8821 ttttcgcatc agcgccgggg taggaggcgg agatcgttac tgccggcggt gcaatcgtag
     8881 gatattgcgc caccggcagt ttgaggatcg ccagcccccc tgccaacatg atgataatgg
     8941 cgatcaccca cgcaaaaatc gggcgatcga taaagaaatt aggcatgtct taacggctcc
     9001 tgtttaagtt aagacttgga ctgttcaggc tgagcaccgc ttgcggcttg ctggttatta
     9061 tcagcggtaa cttcttgtgc ttttacctgg acaccaggac gcactttctg cagcccactt
     9121 attactacgc gatcgcctgc tttcagacct tctgtcacca gccacttatc gccaatagcc
     9181 tggcttgcaa cgatcggacg ggtttccact ttgtcatccg cgccaactac cagtacggtg
     9241 gcatcaccac gcggcgtacg ggttacgccc tgttgcggga ctaaaatagc gtttggatta
     9301 agcccttctt ccagacgtgc gcgcacgaac atacccggca gcagagtgtg atccgggttc
     9361 gggaagatag cgcgtagggt gatagaccca gtggtctgat caacggtaac gtcagagaat
     9421 tccagcgtac cgtcctgcgg gaacttaatg ccgtcactgg tgatcagcga cactttggct
     9481 ttgccgttct cttgtatcag cgtgccattc gccagttcct gtttcaggcg caggaagtcg
     9541 ttgctggact gggtcacatc aacgtagatc ggatcaagtt gctgcacggt tgccagcgca
     9601 gtcgcctgac cgttctgtac caatgcgcct tccgtcacgt tcgacttacc aatgcgaccg
     9661 ctaatcggag aggtgacttt ggtgtaagcc agattgatcc gcgcagtttc aacggcagct
     9721 ttcgccgcag ttaccgcagc attcgcctgt tgcgcatcag ccagagcctg atcgtactct
     9781 tgcttactga tgtactgagt accgagcagt ttctgataac gattcaccgt caattgcgcg
     9841 atattggctg cagcctgggc tttcgccaga tcacctttcg cactgtcgta tgtcgcctga
     9901 taggtcgcag gatcaatctg atagagagag acaccagctt cgatgtcgct accttctttg
     9961 aaattacgct tcaggataat cccgctaact tgaggacgaa cttctgcgat ccggtaggca
    10021 ctggtgcgac ccggaagctc ggttgtgatc tgcagaggtt cagttttgac tgttactacg
    10081 ccaacggcgg gcatctgctg gccaccttgt tgggcctgtt tgtcgtcaca tcctgttagg
    10141 gctaagctgc ctgagagcat cagaacgacc gccagaggcg taaaccctct gtttttgttc
    10201 atatgtaaac ctcgagtgtc cgatttcaaa ttggtcaatg gtcaaaagtt aataaaccca
    10261 ttgctgcgtt tatattatcg tcgtgctatg gtacatacat tcacaaatgt atgtaaatct
    10321 aacgcctgta aattcacgaa catatggcac gaaaaaccaa acaagaagcg caagaaacgc
    10381 gccaacacat cctcgatgtg gctctacgtc ttttctcaca gcagggggta tcatccacct
    10441 cgctgggcga gattgcaaaa gcagctggcg ttacgcgcgg tgcaatctac tggcatttta
    10501 aagacaagtc ggatttgttc agtgagatct gggaactgtc agaatccaat attggtgaac
    10561 tagagcttga gtatcaggca aaattccctg gcgatccact ctcagtatta agagagatat
    10621 taattcatgt tcttgaatcc acggtgacag aagaacgacg tcgattattg atggagatta
    10681 tattccacaa atgcgaattt gtcggagaaa tggctgttgt gcaacaggca caacgtaatc
    10741 tctgtctgga aagttatgac cgtatagaac aaacgttaaa acattgtatt gaagcgaaaa
    10801 tgttgcctgc ggatttaatg acgcgtcggg cagcaattat tatgcgcggc tatatttccg
    10861 gcctgatgga aaactggctc tttgccccgc aatcttttga tcttaaaaaa gaagcccgcg
    10921 attacgtcgc catcttactg gagatgtatc tcctgtgccc cacgcttcgt aatcctgcca
    10981 ctaacgaata accctgaatc tgactccagg atttttcctg gacattttcg tcgttgctat
    11041 tctggttcac tgcgtcgtga tattcttgcg gtttgacttt ttcaggtcgt tcttcaggtt
    11101 cagaaacctt cattcatcat gactatgttc cagtattaca aacgatcacg gcattttgtt
    11161 ttttcagcat ttattgcttt tgtttttgtc ttgttatgcc agaacacggc gtttgcgcgg
    11221 gcgtcatcga atggtgatct gccgacaaaa gcggacctgc aggcgcaact tgactcacta
    11281 aataaacaaa aagatctttc tgctcaggac aaactggtgc agcaggatct gacagataca
    11341 ttagccaccc tcgataaaat cgatcgcata aaagaagaga cagttcagct acggcaaaaa
    11401 gtcgctgaag cgccggaaaa aatgcgccag gcgaccgcgg cgttaacagc acttagcgat
    11461 gtcgataacg acgaagaaac gcgcaaaatt ctgagtacgc tttcgttgcg ccagctggaa
    11521 actcgcgttg cccaggcgct ggacgatttg caaaacgcac aaaacgatct ggcgtcttat
    11581 aacagccagc tggtttcgtt acagacgcag cccgaacgcg tgcaaaatgc gatgtataac
    11641 gcttcgcagc agctgcaaca aattcgcagt cgtctggatg ggactgatgt cggcgagact
    11701 gccttacgtc ccagccagaa agtgttaatg caggctcagc aggcgttgct gaatgcggag
    11761 attgaccagc agcgtaaaag cctggaaggg aacaccgtct tgcaggatac cttgcaaaag
    11821 caacgtgatt acgtgacggc gaacagcgcc cgtctggagc accagttgca gctgttgcaa
    11881 gaagcggtaa acagcaagcg cctgacttta actgaaaaaa cggcgcagga agccgtctcc
    11941 ccggatgaag ccgcgcgtat tcaggctaat ccgctggtga aacaggaact ggaaattaac
    12001 cagcagttaa gtcagcgtct gattaccgcg actgaaaacg gtaatcagtt gatgcagcaa
    12061 aacattaaag tcaaaaactg gctggagcgg gcgctgcaat cggaacgcaa tattaaagag
    12121 cagattgccg tcctgaaggg cagcctgctg ttgtctcgta tcctttacca gcaacaacaa
    12181 acgctgccct cggcggatga actggaaaac atgaccaacc gcatcgcgga tttgcgtctc
    12241 gaacagtttg aagttaacca gcagcgtgat gcactcttcc agagcgatgc gttcgtcaac
    12301 aaactggaag aaggtcacac caacgaagtc aacagcgaag ttcacgatgc gttattgcaa
    12361 gtggttgata tgcgtcgcga attgctggat caactcaaca aacagttggg taaccagctg
    12421 atgatggcca ttaacctgca aatcaaccag cagcagttaa tgagcgtatc gaaaaacctg
    12481 aaatccatcc tgactcagca aatcttttgg gtgaacagta accgtccaat ggactgggac
    12541 tggatcaaag cgttcccgca aagcctgaaa gatgaattta agtcgatgaa aatcacggtg
    12601 aactgggaaa aagcctggcc tgccgttttt atcgctttcc tcgccggttt gccgctgctg
    12661 ttgattgccg ggctgatcca ctggcgtctg ggctggctga aagcgtatca acagaaactg
    12721 gcttccgctg tgggttccct gcgtaacgat agccagctca acacaccaaa agcgatcctt
    12781 atcgacctga tccgcgcgct gccggtgtgc ctgattattc tcgcggttgg cctgattctg
    12841 ttgaccatgc agctcaacat cagcgaactg ctatggtcgt tcagtaaaaa actggcgatt
    12901 ttctggctgg tgtttggcct gtgctggaag gtactggaga aaaatggcgt tgccgtgcgt
    12961 cacttcggca tgccggagca gcagaccagc cactggcgtc ggcaaattgt ccgcatcagt
    13021 ctcgcgttgc tgcctatcca tttctggtct gtggtggcag aactttcccc gctgcatctg
    13081 atggatgatg tgctggggca ggcgatgatt ttcttcaacc tgctgctgat tgccttcctg
    13141 gtatggccaa tgtgccgcga aagctggcgt gataaagagt cgcacaccat gcgactggtc
    13201 accattaccg tgctgtcgat tatcccgatt gcattgatgg tgctgactgc cacgggttat
    13261 ttctacacca cgctgcgcct ctccgggcgt tggattgaaa ccgtttatct ggtgatcatc
    13321 tggaacttgc tctaccagac ggtattgcgc ggcttaagcg tagcggcgcg gcgtatcgcc
    13381 tggcgtcgtg cgctggcgcg tcggcagaat ctggtgaaag agggcgcaga aggtgccgaa
    13441 ccgccggaag aacccaccat tgcactggag caagttaacc agcagacgct gcgtattacc
    13501 atgttgctga tgtttgcgct gttcggtgtc atgttctggg caatttggtc cgatttgatc
    13561 accgtgttca gctatctcga cagcatcacg ctctggcatt acaacggcac tgaagctggc
    13621 gctgcggtgg tgaaaaacgt caccatgggc agtctgttgt ttgcgattat cgcctcaatg
    13681 gtggcctggg cgttgattcg caacctgcct ggtttactgg aagtgctggt gctctcgcga
    13741 ctgaatatgc gccagggcgc gtcgtatgcc attactacca tccttaacta catcattatt
    13801 gccgttggtg cgatgacggt gttcggatcg ctgggcgtct cttgggataa actccagtgg
    13861 ctggccgcag cattatccgt aggtcttggt tttggtttac aagaaatttt cggtaacttc
    13921 gtctccggtt tgatcattct gttcgaacgt ccggtgcgta ttggcgatac ggtaaccatt
    13981 ggtagcttct cggggacggt aagtaagatc cgtattcgtg cgacaacgat taccgatttc
    14041 gatcgcaaag aagtgatcat cccgaacaaa gcgtttgtta ccgagcgtct gatcaactgg
    14101 tcgttgactg acactactac gcgtctggtg atccgtctcg gcgtggccta tggctccgat
    14161 ctggaaaaag tgcgtaaagt gttactgaag gcggcgactg agcacccaag ggtgatgcac
    14221 gaaccaatgc cggaagtctt ctttacggca tttggtgcca gcacgttgga tcatgagctg
    14281 cgtctgtatg tgcgtgaact gcgtgaccgt agccgtactg tcgatgagct gaaccgtact
    14341 atcgatcagc tgtgccgtga aaacgacatc aacattgcct ttaaccagct tgaagtgcat
    14401 ctgcacaacg agaagggcga tgaggtgacg gaagtaaaac gcgactacaa aggcgacgat
    14461 ccgacaccag cggttgggta ataaccaaag ggggcgacat ttagttgccc cgagattact
    14521 gacaaagtgc gcgttgttca tgccggatgc ggcgtgaccg ccttatccgg cctacgaaac
    14581 cgcaagaatt caatatattg caggagcggt gtaggcctga taagcgtagc gcatcaggca
    14641 gttttgcgtt tgcccgcaat cttaggggac atttagcggc cccatttatt tctcactttt
    14701 ccgcctcatc atcgtgcgtt aatttctttt cataatcacg ctttacaata tccagcgcgc
    14761 gcagcacggt actggcaggg atctgatttt cctccagcag cacaatcaaa tcgacagcca
    14821 gtttgacatc gtcaggggca ttttcaagtg acatactctc tccattgcta gcgggttaag
    14881 cgcgctagcc tgttttcgat tttttccagc gcatggcggc agcgtgccag acgcgcttca
    14941 taggcttcca cttcacgatg cagcgtttgc tgttccacga gatcggtcac ccgcgccagg
    15001 cgggctctgc gttcggcaac catctcacgt agccgccgct caaaatcctg atgctgaata
    15061 cgtttacgct gccagcgggc aattttcggt ggcgcactgt cccactcgcg caatgaccag
    15121 gcggaggctt cacgcgcgat ggcttccagt tgtgccgcca gatgttccgc cagccaggcc
    15181 acttgcggca gctgttgctg ctcaactgca tgacgaagcg cagccagatt atcgcccgcc
    15241 tcgtcgagac aagcctgtag tgttgtcgca cgagtctgaa aaagatgcct gtcgaaacga
    15301 gcacttagcg tggcgaactg tgacaccggg gcacaacgct gacgcagcgt agcgagctgt
    15361 ccttccagtt tttccagcag cagggcggtt ttcacgataa gacctcaatg aaaatgataa
    15421 ttgttatgct aaagtagcca ctctgtaagc tgacaacact caatatgcaa cgaatcattt
    15481 taatcatcat tggctggctg gcggtagtgc tgggtacgct gggcgtggta ttaccggtat
    15541 taccgacgac gccgtttatc ctgctggcgg cctggtgctt tgcccgttct tccccgcgct
    15601 ttcacgcctg gttgctgtac cgctcatggt ttggcagcta tctacgtttc tggcagaaac
    15661 atcatgcgat gccgcgcggc gtcaaaccgc gggcgatttt gcttattttg ctcacgtttg
    15721 ccatttctct gtggttcgtc cagatgccat gggtgcgcat catgttgctg gtaattctcg
    15781 cctgtttgct tttctatatg tggcgaattc cggtgattga tgaaaagcaa gaaaagcact
    15841 gaagcacaac aatcgcagtt gcaattattg cgtacagcca gtacattctg gcgttttcga
    15901 gcacaggcgc aggcggtcaa aggttaaaca acagttactt ttgatacgtt taaaacgcgc
    15961 cgtgagtacc accgtaacaa gcaggcatac acttatgacc gcgactgcac agcagcttga
    16021 gtatctcaaa aatagcatca aaagcattca ggactaccca aaacccggca ttcttttccg
    16081 cgatgtcacc agcttactgg aagacccgaa agcttacgct ctcagcatcg acttgctggt
    16141 tgagcgttac aaaaatgcgg gcattaccaa agttgtcggc accgaagcgc gtggcttctt
    16201 gtttggcgct ccggtagctc tgggtctggg cgttggcttt gtaccggtcc gtaaaccggg
    16261 caaactgccg cgtgaaacca tcagtgaaaa ttacgacctg gaatacggca ccgatcagct
    16321 ggaaatccac gttgatgcca tcaaaccggg cgacaaagtt ctggtggtgg acgatctgct
    16381 ggcaaccggc ggcactatcg aagcgaccgt taaactgatc cgtcgtctgg gtggtgaagt
    16441 ggctgacgct gcgttcatta tcaacctgtt cgatctcggc ggcgaacagc gtctcgaaaa
    16501 acagggcatt accagctaca gccttgtccc gttcccgggc cattaattat cgccagtctt
    16561 gtgctgccca cgctacggac agcacaagat gtgcattcag cctcgccgtt ctgacggggc
    16621 tgtgttagca ttaccccttc gtgaatccac cttccagcgt ttcagagcct gccaatgagt
    16681 tatcaggttt tagcccgaaa atggcgccca caaacctttg ctgacgtcgt cggccaggaa
    16741 catgtgctga ccgcactggc gaacggcttg tcgttagggc gtattcatca tgcttatctt
    16801 ttttccggca cccgtggcgt cggaaaaacc tctatcgccc gactgctggc gaaggggcta
    16861 aactgcgaaa ccggcattac cgcgacgccg tgcggcgtgt gcgataactg tcgtgaaatc
    16921 gagcaggggc gctttgtcga tctgattgaa atcgacgccg cctcgcgcac caaagttgaa
    16981 gatacccgcg aactgctgga taacgtccag tacgctccgg cgcgtggtcg tttcaaagtt
    17041 tatctgatcg acgaagtgca tatgctgtcg cgccacagct ttaacgcact gttaaaaacc
    17101 cttgaagagc cgccggagca cgttaagttt ctgctggcga cgaccgatcc acagaaattg
    17161 ccggtgacga ttttgtcacg ctgtctgcaa tttcatctca aggcgctgga tgtcgagcaa
    17221 attcgccatc agcttgagca catcctcaat gaagaacata tcgctcacga gccgcgggcg
    17281 ctgcaattgt tggcgcgcgc cgctgaaggc agcctgcgag atgccttaag tctgaccgac
    17341 caggcgattg ccagcggtga cggccaggtt tcaacccagg cggtcagtgc gatgctgggt
    17401 acgcttgacg acgatcaggc gctgtcgctg gttgaagcga tggtcgaggg caacggcgag
    17461 cgcgtaatgg cactgattaa tgaagccgct gcccgtggta tcgagtggga agcgttgctg
    17521 gtggaaatgc tcggcttgtt gcatcgtatt gcgatggtac aactttcgcc tgctgcactt
    17581 ggcaacgaca tggccgccat cgagctgcgg atgcgtgaac tggcgcgcac cataccgccg
    17641 acggatattc agctttacta tcagacgctg ttgattggtc gcaaagaatt accgtatgcg
    17701 ccggaccgcc gcatgggcgt tgagatgacg ctgctgcgcg cgctggcgtt ccatccgcgt
    17761 atgccgctgc ctgagccaga agtgccacgc cagtcctttg cacccgtcgc gccaacggca
    17821 gtaatgacgc caaccctggt gccgccgcaa tcagcgccgc agcaggcacc gactgtaccg
    17881 ctcccggaaa ccaccagcca ggtgctggcg gcgcgccagc agttgcagcg cgtgcaggga
    17941 gcaaccaaag caaaaaagag tgaaccggca gccgctaccc gcgcgcggcc ggtgaataac
    18001 gctgcgctgg aaagactggc ttcggtcacc gatcgcgttc aggcgcgtcc ggtgccatcg
    18061 gcgctggaaa aagcgccagc caaaaaagaa gcgtatcgct ggaaggcgac cactccggtg
    18121 atgcagcaaa aagaagtggt cgccacgccg aaggcgctga aaaaagcgct ggaacatgaa
    18181 aaaacgccgg aactggcggc gaagctagcg gcagaagcca ttgagcgcga cccgtgggcg
    18241 gcacaggtga gccaactttc gctaccaaaa ctggtcgaac aggtggcgtt aaatgcctgg
    18301 aaagaggaga gcgacaacgc agtatgtctg catttgcgct cctctcagcg gcatttgaac
    18361 aaccgcggtg cacagcaaaa actggctgaa gcgttgagca tgttaaaagg ttcaacggtt
    18421 gaactgacta tcgttgaaga tgataatccc gcggtgcgta cgccgctgga gtggcgtcag
    18481 gcgatatacg aagaaaaact tgcgcaggcg cgcgagtcca ttattgcgga taataatatt
    18541 cagaccctgc gtcggttttt cgatgcggag ctggatgaag aaagtatccg ccccatttga
    18601 tcgtaagcac agcttacgtt cgtcatcctt aacgtgattg agagagaaac ctatgtttgg
    18661 taaaggcggt ctgggtaacc tgatgaagca agcccagcag atgcaagaaa aaatgcagaa
    18721 aatgcaggaa gagatcgcgc agctggaagt taccggcgaa tctggcgcag gtctggtaaa
    18781 agtgaacatc aacggtgcac acaactgccg tcgcgtagag atcgacccga gcctgctgga
    18841 agacgacaaa gagatgctgg aagacttggt ggctgcagca ttcaacgacg cagcacgtcg
    18901 tattgaagaa acgcagaaag aaaaaatggc ctctgtatcc tccggaatgc agctgccgcc
    18961 tggctttaag atgccgttct gatgcaaacc agcccgctgt taacacagct tatggaagca
    19021 ctgcgctgtc tgccgggcgt tggcccgaag tcggcgcagc gtatggcgtt cacgctgctt
    19081 cagcgcgatc gtagcggcgg gatgcgtctg gcgcaggcgc tcacccgggc gatgtcggaa
    19141 atcggccact gcgccgattg ccgcactttc accgaacagg aagtctgtaa catctgttcg
    19201 aatccgcgtc gtcaggaaaa cggtcaaatc tgtgtggtgg agagtccggc ggacatctac
    19261 gccattgagc agacggggca gttttctggt cgttattttg tgttgatggg gcatctgtca
    19321 ccgctggacg gcatcggtcc ggatgatatc gggcttgatc gtctggaaca gcgtctggca
    19381 gaggaaaaaa tcactgaagt gatcctcgcc accaatccca cggttgaagg tgaagctacc
    19441 gctaactaca ttgccgagct ttgcgcgcaa tatgacgtgg aagccagccg aatcgctcat
    19501 ggcgtaccgg ttggcggcga actggaaatg gtcgatggca ccacgctgtc acactccctc
    19561 gccgggcgtc ataagattcg tttttaagca aacgagagca ggagcacctg ctctcgcttg
    19621 aaattattct cccttgtccc catctctccc tcatcctgtt tttaacctta aaatggcatt
    19681 attgaggtag acctacatga aaggacaaga aactcgtggt tttcagtcag aagtgaaaca
    19741 gcttctgcac ctgatgatcc attctctcta ttccaataaa gaaatcttcc tgcgtgagct
    19801 tatctctaac gcctccgatg cggcggacaa gctgcgtttc cgtgcgctat ctaacccgga
    19861 cctgtacgaa ggtgatggcg aactgcgcgt tcgtgtctct ttcgataaag acaagcgcac
    19921 gctgaccatc tccgataacg gcgtggggat gacccgcgac gaagtgattg accatctggg
    19981 gactatcgct aaatccggta ccaaatcatt cctcgaatcc ctgggttctg accaggcgaa
    20041 agacagccag ctgatcggtc agtttggtgt tggtttctac tctgcgttta tcgtggccga
    20101 caaagtgacc gtgcgtactc gcgcggcagg cgaaaaacca gaaaacggcg tcttctggga
    20161 atcggctggc gaaggtgaat ataccgttgc cgacatcacc aaagaagatc gtggtactga
    20221 aatcaccctg catctgcgtg aaggcgaaga cgagttcctc gatgactggc gcgtgcgttc
    20281 catcatcagc aaatactccg accatatcgc gctgccggta gagatcgaaa aacgcgaaga
    20341 gaaagatggc gaaaccgtta tctcctgggc aaaaatcaac aaagcgcagg cgctgtggac
    20401 tcgtaacaag tcggaaatca ccgatgaaga gtataaagag ttctacaaac acatcgccca
    20461 cgactttaac gatccgctga cctggagcca caaccgcgtt gaaggtaagc aggagtacac
    20521 cagcctgctg tacatcccgt cccaggctcc gtgggatatg tggaaccgtg atcataaaca
    20581 cggcctgaaa ctgtacgttc aacgcgtgtt tatcatggac gacgctgaac agttcatgcc
    20641 gaactatctg cgcttcgtgc gtggtctgat tgactccagc gatctgccgc tgaacgtttc
    20701 ccgtgaaatc ctccaggaca gcacggtaac gcgtaacctg cgcaatgcgc tgaccaagcg
    20761 tgtgctgcaa atgctggaaa aactggcgaa agacgacgcg gaaaaatacc agaccttctg
    20821 gcaacagttt ggcctggtac tgaaagaagg tccggcggaa gatttcgcta accaggaagc
    20881 gatcgccaaa ctgctgcgtt ttgcttctac ttataccgat tcttctgcgc agaccgtgtc
    20941 tctggaagac tacgtttccc gcatgaaaga agggcaggag aaaatctact acatcactgc
    21001 agacagctat gcggcagcga agagcagccc gcacttggaa ctgctgcgta agaaaggcat
    21061 cgaagttctg ctgctttccg accgcatcga tgagtggatg atgaactatc tgactgagtt
    21121 cgacggtaaa ccgttccagt cggtgtctaa agttgacgag tcgctggaaa aactggctga
    21181 cgaagttgat gagagcgcga aagaagcgga gaaagcactg actccgttca tcgaccgcgt
    21241 gaaagctctg ctcggcgagc gcgtgaaaga tgtccgtctg actcaccgtc tgaccgatac
    21301 gccagcgatc gtttcgaccg acgcggacga aatgagcact cagatggcga aactgttcgc
    21361 tgcggcgggc cagaaagtgc cagaagtgaa atacatcttc gaactgaacc cggatcacgt
    21421 actggtgaaa cgtgcggcag atactgaaga tgaagccaag ttcagcgagt gggtagaact
    21481 gctgctggat caggcgctgc tggcagaacg cggcacgctg gaagatccga acctgtttat
    21541 tcgtcgtatg aaccagctgc tggtttcctg atgtcatgcc ggatgacctt tgtgtcatcc
    21601 ggcatttttc ttttcatcat ctgcactttc cgcaaattat ctcgccatta accgtttcag
    21661 cctcaggtgc ctttcttgag gcaatcgcct gttggtggta tcgtttatcg ctttttcaaa
    21721 aaaatcgaca cattttaagg ggattttcgc aatgcgtatc attctgcttg gcgctccggg
    21781 cgcggggaaa gggactcagg ctcagttcat catggagaaa tatggtattc cgcaaatctc
    21841 cactggcgat atgctgcgtg ctgcggtcaa atctggctcc gagctgggta aacaagcaaa
    21901 agacattatg gatgctggca aactggtcac cgacgaactg gtgatcgcgc tggttaaaga
    21961 gcgcattgct caggaagact gccgtaatgg tttcctgttg gacggcttcc cgcgtaccat
    22021 tccgcaggca gacgcgatga aagaagcggg catcaatgtt gattacgttc tggaattcga
    22081 cgtaccggac gaactgattg ttgatcgtat cgtaggccgc cgcgttcatg cgccgtctgg
    22141 tcgtgtttat cacgttaaat tcaatccgcc gaaagtagaa ggcaaagacg acgttaccgg
    22201 tgaagaactg actacccgta aagacgatca ggaagaaacc gtgcgtaaac gtctggttga
    22261 ataccatcag atgactgcac cgctgatcgg ctactactcc aaagaagcgg aagcgggtaa
    22321 caccaaatac gcgaaagttg acggtaccaa gccggttgct gaagttcgcg ctgatctgga
    22381 aaaaatcctc ggctaatcaa gccgcagcag atctgctttc gggcaggtct gctcatccac
    22441 ctcaggcaat acctctcaca gcaattagtt cttcttcctc acttttccgc tacaattatc
    22501 aacaagttga atcgataaga ggcggtaatg cgtcagacta aaaccggtat cctgctggca
    22561 aacctgggta cgcccgatgc ccccacacct gaagcggtaa aacgctatct gaaacaattt
    22621 ttaagcgaca gacgcgtggt tgatacctca cggttgttat ggtggccgtt gctgcgcggc
    22681 gtgattttgc cgctgcgctc gccgcgtgtg gcgaagctgt acgcctctgt ctggatggaa
    22741 gacggctcgc cgctgatggt ttacagccgc cagcaacagc aggcgttggc acaacgttta
    22801 ccggatacgc cagtggcgct gggcatgagc tacggctcgc catcactgga aagcgccgtc
    22861 gatgaactcc tggcagagca tgtagatcat attgtggtgc tgccgcttta tccgcaattc
    22921 tcctgttcta cggtcggtgc ggtatgggat gaactggcac gcattctggc gcgcaaacgt
    22981 agcattccgg ggatatcgtt tattcgtgat tacgccgata accacgatta cattaatgca
    23041 ctggcgaaca gcgtacgcgc ttcttttgcc aaacatggcg aaccggatct gctgctgctc
    23101 tcttatcatg gcattcccca gcgttatgca gatgaaggcg atgattaccc gcaacgttgc
    23161 cgcacaacga ctcgcgaact ggcttccgca ctggggatgg caccggaaaa agtgatgatg
    23221 acctttcagt cgcgctttgg ccgggaaccc tggctgatgc cttataccga cgaagcgctg
    23281 aaaatgctcg gagaaaaagg cgtaggtcat atacaggtga tgtgcccggg ctttgctgcg
    23341 gattgtctgg agacgctgga agagattgcc gagcaaaacc gtgaggtctt cctcggtgcg
    23401 ggcgggaaaa aatatgaata tattccagcg cttaatgcca cgccggaaca tatcgaaatg
    23461 atggctaatc ttgttgccgc gtatcgctaa agctgagcgg taaagaactg agcgccgtcg
    23521 cgaagagcct cgtcggcggt tttcatcatc cgtgaataat gcaaaaaggc atgcagcgta
    23581 cctgggtaga gtttgaactc acagggctgc tgatgcgccg ctaacgtctg gtaaagcaga
    23641 cggctgtcat ccagcagcgg atcgaactcc gccccggcaa taaaacaggg cggaacttcg
    23701 cgagtgagat cattattaaa cagacagtaa tacggtgact cgcggtccgc gtcgttgctt
    23761 aaatacgcct cttcatacat ctgcaaatcc tgttgcgtta agccatccca gacaccgccc
    23821 aacagacgac gagtcacgga atcccgtaat ccgtaaagcc cataccacag caaaacaccc
    23881 gcaattttac cgcaatcgat ctgtttatca cgcaaccaca acgcactggc gagcgccagc
    23941 atggcacctg cggaatcacc ggcaaagcca atgcgggaca tattgatttg ataatcctcc
    24001 gcctgctggt ggaaataaca acaagcagcc acaatttcct ctatcgcttg tggaaaacgc
    24061 gcttcaggtg aaagggtgta atcaatacca atcaccgtac attggctgta gcttgccagc
    24121 aggcgcatga tgcgatcgtg ggtatcgaga ttgccgagaa taaaaccgcc tccatgcaaa
    24181 taaaatagcg tcgccgggct atctggctgc ggacaaaaga gacgtgtttc cacctgccca
    24241 tattttgttg gaaccatgta agctctggtt gccatttctg gagcgcccgc attccagaat
    24301 cggcgctcaa gcgtgtaata ctgtcgttgc tcagcaatcg ttcccgttgc gggccaggac
    24361 ggtaaatccg gctgaagagt attcacaacg gtcttcattt cagcagaaat aaggtccaga
    24421 acaggtagtt tgttttccgg cttcatacaa aaactccttt caaattacgt cattgtaagg
    24481 aaccactgcc atgaaaatgc gatcccgcct gctgatattg aaactggctg cgtctcgcgc
    24541 gctcccgtca gattgtgtta acattcgccg ctcagttaac cacccgtaaa aacaaccatg
    24601 aaatttcccg gtaaacgtaa atccaaacat tacttccctg taaatgcacg cgatccgctg
    24661 cttcagcaat tccagccaga aaacgaaacc agcgccgctt gggtcgtagg tattgaccag
    24721 acgctggtcg atattgaagc gaaagtggat gatgaattca ttgagcgtta tggattaagc
    24781 gccgggcatt cactggtgat tgaggatgac g
//
LOCUS       DBKEKI010000054        24705 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.054, whole genome shotgun sequence.
ACCESSION   DBKEKI010000054 DBKEKI010000000
VERSION     DBKEKI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.054"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(45..4661)
                     /locus_tag="ACUQPX_002138"
                     /pseudo
     CDS             complement(45..4661)
                     /locus_tag="ACUQPX_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(4754..5401)
                     /gene="rnt"
                     /locus_tag="ACUQPX_002139"
     CDS             complement(4754..5401)
                     /gene="rnt"
                     /locus_tag="ACUQPX_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HHV9056848.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5504..5911)
                     /gene="gloA"
                     /locus_tag="ACUQPX_002140"
     CDS             complement(5504..5911)
                     /gene="gloA"
                     /locus_tag="ACUQPX_002140"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HHV9056849.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(5992..7077)
                     /gene="nemA"
                     /locus_tag="ACUQPX_002141"
     CDS             complement(5992..7077)
                     /gene="nemA"
                     /locus_tag="ACUQPX_002141"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HHV9056850.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISDSAQTKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQLWHTG
                     RISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGIVNDF
                     RQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAG
                     IEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGE
                     PYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQ
                     LKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7114..7713)
                     /gene="nemR"
                     /locus_tag="ACUQPX_002142"
     CDS             complement(7114..7713)
                     /gene="nemR"
                     /locus_tag="ACUQPX_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HHV9056851.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            7816..8055
                     /locus_tag="ACUQPX_002143"
     CDS             7816..8055
                     /locus_tag="ACUQPX_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HHV9056852.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8104..9000
                     /locus_tag="ACUQPX_002144"
     CDS             8104..9000
                     /locus_tag="ACUQPX_002144"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HHV9056853.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9081..9602
                     /gene="sodC"
                     /locus_tag="ACUQPX_002145"
     CDS             9081..9602
                     /gene="sodC"
                     /locus_tag="ACUQPX_002145"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HHV9056854.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIYAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9603..11615)
                     /locus_tag="ACUQPX_002146"
                     /pseudo
     CDS             complement(9603..11615)
                     /locus_tag="ACUQPX_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11615..12472)
                     /locus_tag="ACUQPX_002147"
     CDS             complement(11615..12472)
                     /locus_tag="ACUQPX_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HHV9056855.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNVAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(12475..12711)
                     /locus_tag="ACUQPX_002148"
     CDS             complement(12475..12711)
                     /locus_tag="ACUQPX_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HHV9056856.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            12912..13346
                     /gene="slyA"
                     /locus_tag="ACUQPX_002149"
     CDS             12912..13346
                     /gene="slyA"
                     /locus_tag="ACUQPX_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HHV9056857.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLDHNIIELQAKG"
     gene            complement(13393..13860)
                     /gene="slyB"
                     /locus_tag="ACUQPX_002150"
     CDS             complement(13393..13860)
                     /gene="slyB"
                     /locus_tag="ACUQPX_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HHV9056858.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14134..15243
                     /gene="anmK"
                     /locus_tag="ACUQPX_002151"
     CDS             14134..15243
                     /gene="anmK"
                     /locus_tag="ACUQPX_002151"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HHV9056859.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            15341..15670
                     /gene="mliC"
                     /locus_tag="ACUQPX_002152"
     CDS             15341..15670
                     /gene="mliC"
                     /locus_tag="ACUQPX_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HHV9056860.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15729..16385
                     /gene="pdxH"
                     /locus_tag="ACUQPX_002153"
     CDS             15729..16385
                     /gene="pdxH"
                     /locus_tag="ACUQPX_002153"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HHV9056861.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16514..17788
                     /gene="tyrS"
                     /locus_tag="ACUQPX_002154"
     CDS             16514..17788
                     /gene="tyrS"
                     /locus_tag="ACUQPX_002154"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HHV9056862.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            17847..18710
                     /gene="pdxY"
                     /locus_tag="ACUQPX_002155"
     CDS             17847..18710
                     /gene="pdxY"
                     /locus_tag="ACUQPX_002155"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HHV9056863.1"
                     /translation="MMKNILAIHSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(18754..19359)
                     /gene="gstA"
                     /locus_tag="ACUQPX_002156"
     CDS             complement(18754..19359)
                     /gene="gstA"
                     /locus_tag="ACUQPX_002156"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HHV9056864.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19465..20967)
                     /gene="dtpA"
                     /locus_tag="ACUQPX_002157"
     CDS             complement(19465..20967)
                     /gene="dtpA"
                     /locus_tag="ACUQPX_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HHV9056865.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFTLSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(21578..22213)
                     /gene="nth"
                     /locus_tag="ACUQPX_002158"
     CDS             complement(21578..22213)
                     /gene="nth"
                     /locus_tag="ACUQPX_002158"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HHV9056866.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22213..22908)
                     /gene="rsxE"
                     /locus_tag="ACUQPX_002159"
     CDS             complement(22213..22908)
                     /gene="rsxE"
                     /locus_tag="ACUQPX_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HHV9056867.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(22912..23532)
                     /gene="rsxG"
                     /locus_tag="ACUQPX_002160"
     CDS             complement(22912..23532)
                     /gene="rsxG"
                     /locus_tag="ACUQPX_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HHV9056868.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23536..24594)
                     /gene="rsxD"
                     /locus_tag="ACUQPX_002161"
     CDS             complement(23536..24594)
                     /gene="rsxD"
                     /locus_tag="ACUQPX_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HHV9056869.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         6122 a         6390 c         6431 g         5762 t
ORIGIN      
        1 aacgtagcag ggaaccacgt ccttcagggc gtggaggatg tcaactatcc ccaatccagc
       61 ccttgtggcg aacttgaaaa tcccgcctca cgcagcagcg taaacatcgg cgtttgccgg
      121 accggaagat cattcacttc tgttagcgta aagcgcagcc ttggttcgcg acgcagtgcg
      181 gtggtcagcg cctggaaaat ctctggggcg agtaattcct ccttttcctg ccagaccagc
      241 atttttttgc cgccttgcgc cagatagagt aacaatttgc cgccagaaac gaccaccagc
      301 gcacccgccc gacgcgttgg taccagcgtt gccggatgtg caggccaggg cagaagattt
      361 ccccacacat tagccggatc gttggcggag agcgccactg gtgtatagtg gcgtgtttgc
      421 gtggcttgtg tcgccagatc gcgcaatcgg tcaatagtca gacgttcagc gaattgcgcg
      481 ccacccagac cttctacaaa acgacctcgc ataattcgcc cggagtcttc catacttcga
      541 caaagtgttt gcatcgatgg aaacccgcca gggatatttt cggctatcac cgcctgacga
      601 ctgatgatgc cgtagcggtc gagcatattt tccgccagcg ctagcatcct ttcggtatcg
      661 tttagtggct ccacctgcaa taacgaccag cgtccagcca gatttggtgt gttgtaagat
      721 acccgcggcg agacgggttg tgcatagaca ggacgcccgc gacgagcccg gtgactgcgg
      781 cgagttgagg tgcgtgcgtt ggaactgctg cgggtgaggg cgcgtaacgg tgcccaaata
      841 tcgctggtaa tgacgccttg ccagaccagc gcccataacg cctcttgcag tgccgaggaa
      901 tcaaccgatt cgccgatttt atcgcgtatc cgttggctga tttgttgtgc aaaccaggct
      961 cctccgtcag ccagaacagc gattatcgct tgctgcagcg ccgaacgatt cgcctgatcc
     1021 gcttcggcgg gagtgaacga ttctgcggca tatttctgta gatgcagtgc caccaggccg
     1081 tcatcttcac ccagcttttt ttgtcccgac cagataaccg ctccggttgc cagtaattca
     1141 tcgagcattt ctggcgaata gtcgcgtacg cgggcaggca ggatctggct ttcccagagt
     1201 gaggcgggta aaccgactcc ggcaagctgt tcgatcaccc gcatcacgcc atctacgccc
     1261 tcataaacgc ctggctatgt tgaggcaaag agcgccgggc taccatcggt ggcgggtaat
     1321 acgccctgac gttccaacag caatcgcgca taggtcgtgg ctgcaacggg acgcgtcgct
     1381 tctctggcgg cttgcagcga gcgcaaacgc agacgacgaa atacttcatc gctcacccag
     1441 atgtcttgtt gcagattcat caccagaccc tgttcacgca gttgctgaag ctgctcttcg
     1501 acaatggcaa taccgagact aaactcatga gccagttgtt cagccgtgac cagagcatga
     1561 gcccggagat agcgcagaaa gaggtcgcga agcgggtaac tgactctatg taaataaatc
     1621 tctggcaatg actctggtag tcgtacgccg agggcatcac gcagcctggc ggcatcatcc
     1681 atacaggcaa gacgctcctg tccgctaatc atcgctggga agattcgttt tactgcaaga
     1741 agattttcca gatacgacgc aacctcttca ctgctgcctg tatgccgttg cgccaggtct
     1801 tcaacggtca ttggccccag ttcgcgcagc aggtcgaaca atccttcttc acctttcgct
     1861 cttctgccag gagccagtcg ttgcaactct tcttccacct ggcgaatgac ctgcgggtcg
     1921 agtaattccc ccggatcgac ctgtccgagt agattgcgca gtaactcgct gtccagcgac
     1981 agtacggatg cccggcgctc tgccagcggg gcgtcgctct ggtacataaa ttccgcgaca
     2041 tagccgaaca ataaacttgt ggcgaaaggc gagggcgtag tggtcgttac atcggatatt
     2101 tgaatttcgc caccgttcag acgacgcatc aaacgttcca gtgcgggaag atcataaaca
     2161 tcttgcagac attcgcgtag ggtttcgaga atgaccggaa aatccggata tccctgagcg
     2221 atttccagca actgactggc gcgcagccgt tgttgccaaa gcggggtgcg atggcccggc
     2281 gtgcggcccg gcattaataa tgcccgcgcg gcgcattcgc gaaaacgggc ggcgaaaagt
     2341 gccgagctgc ctaccgcctc gcggacaatt tgcagcaact tttctggttc aaacaaaaaa
     2401 atcgcggcgt cgggcaattt accatcagtg tcaggaatgc gcgcaacaat gccgtcatca
     2461 ctggcgacca cagacgcgtc agcgccccat agggcatgta ttcgcccggc aatcgccagc
     2521 gcccagggtt catgcacccg tcttccatag ggagagtgca aaataatacg ccagtcgcca
     2581 atttcatcac ggcaccgttc gaggaccaga tggcgactcc ccggaacgat gcccgtcgcg
     2641 ttgcgttgct cgtcaatcaa cccctgaata ttggcgttcg tattttcttc tgccagccac
     2701 gggggaatag tcccggaaaa gaacgcgcca tccgccagta aatgaagaaa atcgccgatc
     2761 atctcgccta attcagccgg acgtccgtta ccttcaccac gccagaaggg gagccgggca
     2821 gaacgacccg gagcaggagt cacaatcacc tgatcgcggg tgatttgctg gatccgccat
     2881 gaggtagcgc cgagagtgat aatgtcgttc acccgcgact catataccat ctcctcatcc
     2941 agttcaccca cccgccgcga accggccttt tcttcacctt cgggtaataa cacgctatac
     3001 atgccacgat ccggaatggt gccgccgctg gtaaccgcca acaattgagc gccaggtcgg
     3061 gcggtcaata tcccggtctc cctgttccag accagtttgg ggcgaaaagc agaaaaatcg
     3121 ccagagggat agcgcccgga aagcatatcc agcgtggcgt caaaaacacg tcttggcaga
     3181 tctttccacg gtgcggcacg gcgtacgcgg gagtaccatt cgtctacctg taatgcatcc
     3241 atcgccgcgg cggcaacggt ttgctgcgca aggacgtcga gaggattatg cggtggtgtc
     3301 aggttttcca gcctgcctgc gaacatacac tctacaatga ctgcggaatc gactaaatca
     3361 cgccgggtac gggggaaaaa cagcccttta gatacaccgc caacctgatg tccggcgcga
     3421 ccaatgcgtt gtaacccact ggcaacagaa agcggcgttg ccacctgaat caccagatcc
     3481 accgcgccca tatcaatccc cagttcaaga ctggaggttg cgaccacgca gcgtaattca
     3541 cccgatttca gcgcctgttc ggtgattgct cgttgttctt tagagacgga gccgtggtgt
     3601 gagcgggcaa taaaaacgtc gctactttgt acacggttag aggttgcgcc ggaggtcgac
     3661 tcgaaatggg ccgcatcaac ggcgatagac ggggaacgct gtaagcgtgc ggcgtaaagc
     3721 tcatttaatc gtgccgtcag tttttccgcc agcccgcgtg aattagtaaa gacaatggtc
     3781 gagcgatggc gcaacacttc atcaaggata cccgtttcaa tatatggcca gatggagcct
     3841 tcccggccgg catggctgtc ttcgccggtg ccgctggcga ccgatgagac atcatccata
     3901 ttggcgaccg gtacgacaat tcgtatctgc ggatggcgca ttgctggcgg gttgactacc
     3961 gtaaccgggc gatcgccacc aagaaatgct gccacatcgc tggctgagcg cacagtggca
     4021 gaaaggccaa ttcgctgtgc tgaggtgtgg agcagcgcat cgagccgctc cagacttaac
     4081 gccagatgcg caccacgttt actgcccgct accgcgtgga cttcatcaat aattaccgtt
     4141 tcgacgccgc gtagcgtttc gcgcgcgcgg gaggtcagca tcagatagag tgattcgggt
     4201 gtggtgatca gaatatccgg cggattacgg gtgagtttgc tgcgttcctg tgcaggcgta
     4261 tcgccagtac ggatccctac gcgaagattg acttccgttt cgccgcgccg ccgccgttca
     4321 tcggcaatac ccttcaacgg gatctgcaag ttgcgctgaa cgtcggtgcc cagggctttt
     4381 atcggtgaaa tatagaggat gcgtgaggtt ttacgcttat gcgcctcgcg ggtatcttcg
     4441 ccgccttcgc ggaagagccg atcgagggcg tagagaaatg ctgccagcgt tttcccggag
     4501 ccagtcggtg cgatcaccag cgcatgttcg cctcgcgccg ccacatgcca ggtttgcgac
     4561 tggacagcgg tcggctgttt aaaggcgcga agaaaccagt cgcgggtcgc cggtgaaaac
     4621 acgtccggca ggagcgatga agggtctgga ttatctgcca ttaacgctat tccccgtgaa
     4681 tatcatgctt gatgcgtgga atagcctcac tatagatgcc tgaatcattg catgtcatca
     4741 ggcatcgact cgattacacc tcttcggcgg caggtagcgg ccagcctccc agacgtttcc
     4801 agcggttgac gatttcacaa aacagcacag cagtgcgttc ggtgtcgtac agcgcggagt
     4861 gcgcctgggt gctgtcgaag tccatgccag cggtctggca agcctttgac aatacggttt
     4921 gtccgagtgc cagcccggcc agtgcagcag tgtcaaaagt ggcgaaaggg tggaacgggt
     4981 tacgtttcag cgaggcgcgt tctgcggcgg ccatcataaa gctgtgatca aaattggcgt
     5041 tgtgcgccac cataatggcg cggttacagc cgctcgcttt aataccttta cgtacaactt
     5101 taaaaatttc gtgcagcgcc tcgtattcgc tgaccgcgcc gcgatcggga tcgttcgggt
     5161 caatgccgtt gaaggcgagg gcttctggtt gcaaatttgc gccgacaaat ggttcgacgt
     5221 ggaaatgtaa tgtggtgtcc ggcatcagcc agccttgttc atccattttc agggtgatgg
     5281 cggcaatctc aagcagcgca tcggttttgg cgttaaatcc ggctgtttca acatcgatca
     5341 caacaggata aaaaccacga aaacggtcgc acagaccggt aagttgagcg ttatcggaca
     5401 tctgattctc ttaatacgga taaattgcag cgcgcattat gacaaatatt acttgtcgat
     5461 gcagtaaaga tgcgggcgcg atgagttcac gcccggcagg agattagttg cccagaccgc
     5521 gaccggcgtc tttctcttcg attaactcaa ttttgtaacc gtccggatct tccacaaacg
     5581 cgataaccgt agtaccgcct tttaccggac ccgcttcacg ggtcacgtta cccccgtttt
     5641 gacggatttt ttcgcacgct tcagcggcgt tatctacgct aagcgcgatg tgaccataag
     5701 cagtgccgag ttcgtattta tccacgcccc agttgtaggt cagttcaatc accgcttctt
     5761 cggtttccgg gccgtagcca acaaacgcca gtgagtattt gtattccggg ttttcgctgg
     5821 tacgcagcag tttcatgccc agcactttgg tataaaaatc gatggagcgt tgcaaatcgc
     5881 caacgcgcag catggtatga agaagacgca tctttttatc ctcaaaatgg ctaacagatt
     5941 caaaatgttg ttttagtata gcggcggctt tacgccgctc gcaatgttgg attacaacgt
     6001 cgggtaatcg gtatagcctt ccgcgccgcc accgtagaaa ctttcggcac gctgtgggtt
     6061 aagctcagct ttgagctgca agcgggcgac cagatccggg ttcgcaatcc agtcacgacc
     6121 aaatgccacc gcatcaatta accctttgcc gatcagcgtt tcagcttttt ctactgtgta
     6181 tgcacctgcg ccgataatcg gaccgtggaa acgggcgcgt actttttcgc ggaacgcatc
     6241 agtatacggt tcaccccccg cccaatctgg ttctgacata tgcagataag caatgccgcg
     6301 tttacccagt tgttcaatca gatacagtgc atcggcttct tcattcgggc cgttatctgt
     6361 gttctggaaa gtaccgattg gcgaaacgcg aatgccaatg cgatcggcac cccattcttc
     6421 aatcccggca tcgactactt ccagtaccaa acgtgcgcga ttttccacgc tgccgccgta
     6481 ctgatcggta cgatggtttg aagaagggga aaggaactga tgcagcaaat aaccgtgagc
     6541 agagtggagc tctaccagat caaaaccggc ttcacgcgcg ttagcaatgg cctgacggaa
     6601 atcattgacg atacccggaa tctcttccag ttcaagcgca cgcggcatgg atgtttcaac
     6661 gcggatcgcc tgaccatttt catcgcgcag agaagtacgt gttcccgcgc taagtgctga
     6721 aggcgctacc ggtgcctgac cgccaggttg caggctggcg tgagaaatgc gtccggtgtg
     6781 ccacagctgc aaggccatat gaccattttc agcatgaacg ccagcggtga tttttttcca
     6841 tgcggcaatt tgctccggac tatggatgcc aggcgcacct gcatatcctt ttgtctgggc
     6901 agaatcacta ataatcaaac cggcactggc acgttggcga tagtattccg ccatcaacgg
     6961 ggtaggaatg tcacccggtt caatactgcg cagacgcgtc agcggtgcca taaaaatacg
     7021 gtttgccgcc gtgatcgcgc ccactttcag tggggaatac agtttttcag atgacataaa
     7081 ggctcccggt cgtctggtga taaaaaatgc ctgctaaacg gcaggcgtcg caataatgtt
     7141 ttttacatgg gccagcgcgt tttccagtgg ctcgaaactg cgcgaaattt tggcctgcag
     7201 attcgcgcca agccatagtg cgtaaagcac ttgtgcctgt tgtagcggtt cgccacaaaa
     7261 ggttaaacaa tggttctcac ggccattttc cagagcctgc gagagcaggg cgatcacgcc
     7321 gcgagcacct ttatccatcg cgctgcgcat atcttctgac agatcgcaca cttcggcaga
     7381 gagttttact gtcaggcaac cactgatggt tccatgttgg caaaactggt tcagtgtttg
     7441 ctggtaataa gccagtatgc ggtcgcggta gttaccttcg ccggattgca gcaactcagt
     7501 cagtcgctgg tgatatgcgg cgtaatgacg ctcaagcatg gcaacgccaa acgcttcttt
     7561 agagcgaaag tagtgataga aggacccttt cggcacttca gcggttttta gtaattcgct
     7621 tagccccatc ccggtgaatc cacgttgcag gcaaagttgc tcgcccgtcg ccaagagatg
     7681 ttcgcgagta tcatgttcgg tgtgtttgtt catgcgttgg agtgtagtag accagtcggt
     7741 ctagtgcaag caaagttgct agtttggaca ttaacagctt caataatggt aataatctta
     7801 tctgaggagg gcgaggtggc ggagcaatta gagttctttc ccgtccagag cccgtgccgg
     7861 ggaatttgcc agtctgacga acgcgggttt tgtcgcggct gttttcgtag tcgggatgag
     7921 cgttttaact ggaataaaat gagcgatggc gaaaaacaag aggtgctgcg cctttgtcgg
     7981 cagcgattaa tgcgcaaatt acgcgcaaat aagccagcgt catcagacga accggagcaa
     8041 ccatcactct tttgaaccta atcttgcgta tactcctctc atatctgata agaggaagcg
     8101 attatggttc agcgtattac tattgcgccg caaggcccgg agttttcccg ttttgtgatg
     8161 ggctactggc gattgatgga ctggaatatg tccgcccgcc agctggtcag ttttattgaa
     8221 gagcatctgg atctcggcgt gaccaccgtg gaccatgctg atatttatgg tggctatcag
     8281 tgcgaagcgg cgtttggcga ggcactgaaa ctggcacctc acctgcgtga acggatggaa
     8341 atcgtcagta aatgcggtat cgcgacgacc gcgcgtgaag aaaacgtcat tggtcattac
     8401 atcactgacc gcaatcacat cattaagagc gccgaacagt cgctaattaa tctcgcgacc
     8461 gatcatctgg atctgctgtt aatccaccga ccagacccgt taatggatgc cgatgaagtg
     8521 gcggacgcgt tcaaacatct gcatcagagc ggcaaagtgc gtcattttgg cgtatcgaac
     8581 tttacgcctg cgcaatttgc cctgttgcaa tcacgtctgc cgtttaccct tgccactaat
     8641 caggtggaaa tatccccggt gcatcagccg ttactgctgg atggcacgct cgaccaacta
     8701 caacaactgc gtgttcgtcc gatggcgtgg tcctgccttg gtggtggtcg tctgtttaat
     8761 gatgattatt tccagccgct gcgtgatgaa ctggctgtgg tggcggagga gttaaacgcg
     8821 ggctcgattg aacaggtggt ttacgcctgg gtattacgtt taccgtcgca gccgctgcca
     8881 attatcggct caggtaaaat tgagcgcgta cgggcagctg tcgaagcaga aacactgaaa
     8941 atgacccgtc aacaatggtt tcgtatccgt aaagctgcac tggggtatga cgtaccgtaa
     9001 gcacttttag gaatagccgc cgttcaaaaa tgtgtcactg gtttacactt attcaggaat
     9061 gcacaatgaa cggaggtcct atgaaacgtt ttagtctggc cattctggcg ctggttgttg
     9121 cgaccggcgc acaagctgcc agtgaaaaag tcgagatgaa cctcgtcacg tcgcaagggg
     9181 ttgggcagtc aattggtagc gtcaccatta ctgaaaccga taaaggtctg gagttttcac
     9241 ccgatctgaa agcattaccc cccggtgaac atggcttcca tatttatgcc aaaggaagct
     9301 gccagccagc caccaaagat ggcaaagcca gcgccgcgga atccgcaggc gggcatcttg
     9361 atccacaaaa taccggtaaa catgaagggc cagaaggtac cgggcattta ggcgatctgc
     9421 ctgcactggt cgtcaataat gacggcaaag ctaccgatgc cgtcatcgcg cctcgtctga
     9481 aatcactgga tgaaatcaaa gacaaggcgc tgatggtcca cgttggcggc gataatatgt
     9541 ccgatcaacc taaaccgctg ggcggtggcg gtgaacgcta tgcctgtggt gtaattaagt
     9601 aattacgagg ccagcgtacc ttgcggtggt gcttgctcaa gttgcgaaag cgagcagtac
     9661 agccgccaga caattgccgc cagctcacgg gcggcaggtt gatgatgacg ggcaaggctg
     9721 tcgcaaatcc gctgtaattc ttcaagtgtg gccgccagtg atttttgctg aacgccacgc
     9781 tcactcatca ctccccgcaa cagtgaaata cagttatccc gtactcgcga taacggatcg
     9841 gaacgcgatt cccagtcgcg caattgccag acaacatgag aacagttcag cagcactaca
     9901 ccccagcgta ataaccaacg gcgagccagc gcatcctggc tgttactcaa ctgactgacg
     9961 tgatgataag tgagcgattc aaattcgctt tcactcagtg ttggatggcg gcttagctga
    10021 tcgacaaaat cccggcgcag cgcgcgaata tggcggcggc ttttacgagc atccgatcct
    10081 ggacgcagaa tggcgaacgc tagccaagcc agcgcaacgc caacgatttt tgccagatta
    10141 tcgttaagaa aatcagcaaa atcatacacc gggggattag tgacagcgat aaacgaaccc
    10201 ataaaaacaa tcagttgccc ccacagtgcg gcaaatttgg gcatctgcaa tttaagaagc
    10261 tgcattgtcg ccagcagtgg aaagagaaac agtaaaaatt gccacagatc gctaatctgg
    10321 accatcaggc caaatttgac cacaaagctg aatagcgaaa gtaacaccag cgtgcgcatc
    10381 agcagtgaca acgacttaaa cggtgctgcg acggcggagt agagtacgca gctaattgct
    10441 gccagcgtta atgcattggc accggcatcc cattgcgatg caatactcca tgcgccaatc
    10501 atcatcaacg tacaaaatgt acgcagcccg ctccacatag cttcggcgtt gtcggtatga
    10561 cgagccagag cggatgtccg tgcgagtctg gcatgatcat ctacaccgct ttgcagacga
    10621 tgtaattcct gactactttg cagataaagg cggcaaaaat aacgtagtcg ctgccagaag
    10681 gcgacgtgcc ggtagtcggc gacgttggtc gggcgtagcg gggcgataat acgtgcgacg
    10741 gtgtaagcat ctgtttgcga actggcgagc gccgtcagca attgttcgag aatttctcgc
    10801 gtggcacttg gcggtgaggg ccagttgagc aacatacgtc gcaggctgga gatgacactg
    10861 gtcatacgta attgctggtg gagtagcgca ttaaggcgcg cgttttgctg gcgaaaacga
    10921 tagtggctcc agaaagcctg gatacgcagc aaattcatgg tcagtatctg cccaatcacc
    10981 ccctcatgtg ctgcacgaat ggcatcgttt gtttcaggct gccagagtaa actggcatgt
    11041 tccagtaatc gggcgtgcat gtttttcaat gcagttaaaa gggcagtagc atcggaactg
    11101 ctaggcagga tcatcatcat catgccgccg cacaaaatac cgactatcac ctcgcaaacg
    11161 cgcgcctgag cgatatccca cagctggctg gcctcagtaa tattaaccat cggaaaggcg
    11221 atgatggcag ccgtgtagcc cgccagttga aatgcatacg cgacgttatt cgtgaagtgc
    11281 gcacaggcct aggtacaaaa gccaagccac gccgacatgc tcaatagaaa aaaccacggc
    11341 tcattgagcg tatgtccggc aagaagcagt gccgcaatgg ctcccagtaa actgccagcg
    11401 atgcgtccga ggcttttgct gatgacaccg ccaacggtgg gaaagctaac cactgcagcc
    11461 gaggtcatcg cccaataggg ttcatccaga ttcaaataat aggcaaccgt cagcgccaga
    11521 cacatggcaa tggtattgcg taacgcataa cgccattgcg ccagcgtggc cctgaaccag
    11581 ggcaaattgc gcaaggacca tgacgatgcg ttcattattg acctacggca atgctacagg
    11641 tggtgcccga caccagcgtg acatcgcctg gaactttatc cagcgcaaag cggacgggaa
    11701 cgcgttgggc gagacgaacc caggggacgt taggtttcac atccggaata aggcttgagt
    11761 cactttcaac gctctgatca taaatcgccc gaccgatact ggaaacgtga ccctgtaacg
    11821 ttttgttatc actatagagg gtaatttgtg ctggtgcacc ttcgcggata tggcgcaact
    11881 tggtttcttc aaaataacca atgacgtaaa acgagtggct gtcgacaagg gcaaacagcg
    11941 gtttcccggt gtcagcgtaa tcgccgatgc gggtagtgag gttagtcacc catccactca
    12001 ccggggcgcg aatttccgtt tgcgccagtt gccattgtgt ctgtttcaac gtcgcctgtg
    12061 cgacattaac actggcctgc atcgctttaa cattgaggtt agcggtatcg agttcttcgg
    12121 caaaaataaa attttgtgag agatgacgac gacgattcgc ttcgttattg gcttttgcga
    12181 ggtcagattg cgctttcgcc agctgagcct gtgcgttcag ctcggcgatc tgaaagggcg
    12241 ttttatcgat cgtgagcaga agttcccccg cgttaaccag ttggttgtct tttatattca
    12301 gctcaacaat acgtcccgac acctgtgggg tgatagaaac ctgttctgcg cgtattttcc
    12361 cgtcgcgtgt ccacggagat tgcatgtaat aattccacag ccaccatccg gcaagaaccg
    12421 caactatcgc tacaatgatt gttgagaaat acttaattgt tttaattgac atagttacca
    12481 cgcaatcagt attgccagag caaggcaaac gcaaatcgca aacagcgata aatccattaa
    12541 caagggatgc cagatgtcac cggcgtagat ccagccacga agcaggcggt gtacgacaag
    12601 ccagatgacg aatccaaacg cgaatgcttt gaaaaacgga ggaaaataga cggacgcacc
    12661 gaacaccaga tcttgtaagg gcaatcctgt tgcattgagc ataaacttca caaagtagat
    12721 tcctttacga ccgaatatat tgcgttagat taataaatat tctttaagtg cgaaaaattt
    12781 acgcgcaatt tctgaaaaat atgtctgacg gtaaccaaat gcagcaatac atttgtttta
    12841 gcaatacaat tgctgcacac tattctaaaa gccgcataat atcttagcaa gctaattata
    12901 aggagatgaa attggaatcg ccactaggtt ctgatctggc acggttggtg cgcatatggc
    12961 gtgctctgat agaccatcgc ctgaaaccgc tggagttaac acaaacccat tgggttacgt
    13021 tacacaatat ccatcagtta cctccagacc agtcgcaaat tcaactggca aaagcgattg
    13081 gcatcgagca gccatcactg gtccgtactc tggaccaact ggaagaaaaa gggttaattt
    13141 cgcgtcaaac ttgtgccagc gatcgtcggg ctaaacgtat taaactgacg gaaaaggcag
    13201 agccactgat cagcgaaatg gaagctgtta ttaacaaaac ccgcgcggaa atattacatg
    13261 gcatctccgc agaggaactg gagcaactga ttacgctcat cgcaaaactt gatcataata
    13321 tcattgagtt acaggccaaa gggtgaaatg aagggggcgt gtggtcagta acctgaccac
    13381 acgcaaactt atttagcgcg gagaaacggt cacctgactg ccattgctgg ccagtacgac
    13441 acgttggccc ggagagaaac gagtgttgcc ttgtttctgt accaccatga tggtattacc
    13501 atcgtcttta cgaatttcca gctcgacacc ctgcgttttg ttcattgcac tctgtacgcc
    13561 ctgaccagct acgccacctg caacagcgcc tgctgcagta gccagagaac gcccggttcc
    13621 gccaccaaca gtattcccca ggaaaccacc aagaacagca ccgccaattg caccgataac
    13681 gttggaatca tcaccgccct gaatctgtac cggacgtacg ttaacgatgg tgccatagct
    13741 gacattctgt acttgtttcg cttcagaagc ggtataaaca tcccctgaca gggtgtcgtt
    13801 attaacacaa ccgacaagag acagacctac cattgaaaca accaatacgc gtttaatcat
    13861 tgaaaaatct cctgttcacc atgaaacgcc acgcaagcat ccctcatggt caaagtatat
    13921 ggcatatgtg aatccatggt tacatgttct accaaatatg agcaaatcat aatgaaagta
    13981 ggatcaacca attataaaca aaacattcat gatgattatt gatttgaaga taatgtttta
    14041 ctaccaacgg taaaaatcgg tcattccgca tggtattcac gaaaagttta tgtttgagtg
    14101 gtgcgcaaac gtcagattga aaaaggatga actatgaaat cgggccgctt tattggcgtt
    14161 atgtcaggca ccagccttga tggtgttgat gttgtgttgg cgacaattga tgaacaccgg
    14221 gtcgcacagc tggcaagctt gagctggccg atcccggtgt ctctgaaaca ggctgtactg
    14281 gatatttgcc agggccagca gcttacactt tcgcagtttg gacagcttga tactcaactc
    14341 gggcgacttt ttgctgatgc ggtcaatgcc ttgcttaagg aacaaaacct gcaggcgaga
    14401 gatatagttg cgatcggctg tcacggtcaa accgtctggc atgaaccgac gggcgtggca
    14461 ccacacactt tacagattgg cgataacaat caaattgtgg cacgcaccgg aattacggtt
    14521 gtcggtgatt ttcgccgtcg cgatattgcc ttgggaggac aaggcgcacc gttggtacct
    14581 gcgttccatc atgccttgct ggctcaccca accgagcgac gaatggtgct caatattggc
    14641 ggcatcgcca atctgtcact gctcattcct gggcagccgg ttggcggcta cgataccggt
    14701 cctggtaaca tgctgatgga tgcctggatc tggcgtcagg caggtaaacc ttacgataaa
    14761 gatgccgagt gggcacgggc gggtaaagtt attctcccac tgctgcaaaa tatgctcagc
    14821 gacccgtatt tctcgcaacc tgcaccgaaa agcaccggac gcgaatactt taactacggc
    14881 tggctggagc gccatttacg ccattttccg ggtgttgatc cccgtgatgt tcaggccaca
    14941 ctggcagaac tcaccgccgt gaccatttct gaacaagttt tgttgagcgg tggctgcgaa
    15001 cgattgatgg tatgtggtgg aggtagtcgt aatccgctac tcatggcgcg tctggcggca
    15061 ttactgctag gcacagaagt caccaccacc gatgccgttg gcattagtgg cgatgacatg
    15121 gaagcattgg ctttcgcctg gcttgcctgg cggacgctgg cgggattacc aggaaatctg
    15181 ccttccgtca ctggcgcaag ccaggagacg gtactggggg ctattttccc cgccaactcg
    15241 tgatatcaga attaactgaa ttttttctta tcgtgtctgc cgttacactt ggaagattaa
    15301 ggagggtatt tgccctccgg accaggaaag tcttcgggat atgactatga aaaaactgtt
    15361 aattatcatt ttgccggtcc ttctctccgg ttgtagcgcc tttaatcaac tggttgagcg
    15421 aatgcaaacc gatacgctgg aataccagtg tgatgaaaaa ccgttgacgg tcaaactgaa
    15481 taatccgcgc caggaagtca gttttgttta cgataatcaa ctactgcatc tcaaacaggg
    15541 cctttcagcc tctggcgcgc gttactccga cgggatctat gttttctggt cgaaaggtga
    15601 ggaagcgact gtctataaac gcgaccgcat cgtcttgaat aactgtcagt tacaaaatcc
    15661 acagcgttga gatttttcca ggggcggcgc acaatagcgt cacccactga caatccgtaa
    15721 agaaaaccat gtctgataac gacgaattgc agcaaatcgc gcatctgcgc cgtgaataca
    15781 ccaaaggcgg gttacgccgc cgcgatcttc ccgccgatcc attaaccctt tttgaacgtt
    15841 ggctctctca ggcttgtgaa gccaaactgg cggaccctac cgcgatggtg gtcgctaccg
    15901 tggatgaaca tggtcagcct tatcagcgca tcgttttact caaacattac gacgaaaaag
    15961 gcatggtgtt ttacaccaac ctcggtagcc gtaaagcaca tcaaattgaa aataatccgc
    16021 gcgttagcct gctgttcccg tggcataccc ttgaacgcca ggtgatggtg atcggtaaag
    16081 cagaacgact ttcgactctc gaagtgatga aatattttca tagccgcccg cgtgatagcc
    16141 agattggtgc atgggtttcg aagcagtcca gtcgcatttc tgcccgcggt atccttgaaa
    16201 gtaaattcct ggagctgaag cagaagtttc aacagggcga agtgccattg ccgagctttt
    16261 ggggcggttt tcgcgtcagc cttgaacaga ttgagttctg gcagggcggt gagcatcgcc
    16321 tgcatgaccg ctttttgtac cagcgtgaaa atgatgcgtg gaagattgat cgtcttgcac
    16381 cctgaaaaga tgcaaaaatc ttgctttaat cgctggtact cctgattctg gcactttatt
    16441 ctatgtctct ttcgcatctg gcgaaaagtc gtgtaccggc aaaggtgcag tcgttatata
    16501 catggagatt ttgatggcaa gcagtaactt gattaaacaa ttgcaagagc gggggctggt
    16561 agcccaggtg acggacgagg aagcgttagc agagcgactg gcgcaaggcc cgatcgcgct
    16621 ctattgcggc ttcgatccta ccgctgacag cttgcatttg gggcatcttg ttccattgtt
    16681 atgcctgaaa cgcttccagc aggcgggcca caagccggtt gcgctggtag gcggcgcgac
    16741 gggtctgatt ggcgacccga gcttcaaagc tgccgagcgt aagctgaaca ccgaagaaac
    16801 tgttcaggag tgggtggaca aaatccgtaa gcaggttgcc ccgttcctcg atttcgactg
    16861 cggagaaaac tctgctatcg cggcgaacaa ctatgactgg ttcggcaata tgaatgtgct
    16921 gaccttcctg cgcgatatag gcaaacactt ctccgttaac cagatgatca acaaagaagc
    16981 ggttaagcag cgtctcaacc gtgaagatca ggggatttcg ttcactgagt tttcctacaa
    17041 cctgttgcag ggttatgact tcgcctgtct gaacaaacag tacgatgtgg tgctgcaaat
    17101 tggtggttct gaccagtggg gtaacatcac ttctggtatc gacctgaccc gtcgtctgca
    17161 tcagaatcag gtgtttggcc tgaccgttcc gctgatcacc aaagcagacg gcaccaaatt
    17221 tggtaaaact gaaggcggcg cagtctggtt ggatccgaag aaaaccagcc cgtacaaatt
    17281 ctaccagttc tggatcaaca ctgcggatgc cgacgtttac cgcttcctga agttcttcac
    17341 ctttatgagc attgaagaga tcaacgccct ggaagaagaa gataaaaaca gcggtaaagc
    17401 accgcgcgcc cagtatgtac tggcggagca ggtgactcgt ctggttcacg gtgaagaagg
    17461 tttacaggcg gcaaaacgta ttaccgaatg cctgttcagc ggttctttga gtgcgctgag
    17521 tgaagcggac ttcgaacagt tggcgcagga cggcgtaccg atggttgaga tggaaaaggg
    17581 cgcagacctg atgcaggcgc tggtcgattc tgaactgcaa ccttcccgtg gtcaggcgcg
    17641 taaaactatc gcctccaatg ccatcaccat taacggtgaa aaacagtccg atcctgaata
    17701 cttctttaaa gaagaagatc gtctgtttgg tcgttttacc ttactgcgtc gcggcaaaaa
    17761 gaattactgt ctgatttgct ggaaataatg cattaagtgg aaagggggag tgagaaatca
    17821 ctccccctgg tttttataca gggaacatga tgaaaaatat tctcgctatc cactctcacg
    17881 ttgtttatgg tcatgcgggt aacagtgcgg cagagtttcc gatgcgccgc ctgggcgcga
    17941 acgtctggcc gctgaacacc gttcaatttt ctaatcacac ccaatacggc aaatggactg
    18001 gctgcgtgat gccgcccagc catttaaccg aaattgtgca aggcattgcc gccattgata
    18061 aattacacac ctgtgatgcc gtcttaagtg gctatctggg atcggcggag cagggtgaac
    18121 atatcctcgg tatcgtccgt caggtgaaag cagcgaatcc gcaggcgaaa tatttttgcg
    18181 atccggtaat gggtcatccg gaaaaaggct gtatcgttgc accgggtgtc gcagagtttc
    18241 atgtgcggca cggtttgcct gccagcgata tcattgcgcc gaatctggtt gagctggaaa
    18301 tactctgtga gcatgcggta aataacgtcg aagaagcggt tctggcagcg cgcgaactca
    18361 ttgcgcaagg gccacaaatt gtgttggtta aacacctggc gcgagctggc tacagccgtg
    18421 accgttttga aatgctgctg gtcaccgccg atgaagcctg gcatatcagc cgtccactgg
    18481 tggattttgg tatgcgccag ccggtaggtg ttggtgatgt gacgagcggt ttactgctgg
    18541 tgaaactgct tcagggggca acgctgcagg aggccctgga acatgtgacc gctgcagtct
    18601 acgaaatcat ggtgaccacc aaagcaatgc aggaatatga gctgcaagtg gtggctgctc
    18661 aggatcgtat tgccaaacca gaacattact tcagcgcaac aaagctctga aatatctcat
    18721 ttcggcccac aacgacctgt gggccgatgg ctattacttt aagccttccg ctgacagcgc
    18781 gtcttgtact tccggacgtt cagccatacg ttgcataaat gccgcaatgt gctctaaccc
    18841 ttccagattc agtttcaccg cgtatgccca gcgcagaacc gtaaacagat aggcatcagc
    18901 aattgtaaat ctttgcccgc agatccagtg ctcatccttc agtgcctcgt tcacatattg
    18961 cagcttctta tccagctgcg cgcgaactgt cggtttgtac tcttccggtg tatctgggcg
    19021 aaacagaggt gtgaaacctt tatgcagctc ggtggcgatg taattcagcc attcgatggt
    19081 tttatagcgg gaaatactgt ttaccggtgc cagcaactgg cggtcgggga cgctgtcggc
    19141 aagatactgc ataatcgcaa cgccttccgt cagcaaagta ccatcgtcca gcagcaatgc
    19201 aggcacctgc cccttagggt taacggcaaa gtaatcgtca ccgttttcga gacgtttttt
    19261 cattaaatcc acactgacga gcgtaaaatc ctttccgctc tcacgcaggg tgatatggga
    19321 agcgagagag caggcgcccg gtttgtagaa caatttcatc ggtaactcct tttgtctgtg
    19381 gtttaaagta tggtagtgcg ttgctgaaca aaaaaaaagc cgctaacgaa aagttagcgg
    19441 cttgtaaaag agtttccctg aattttacgc tacagcagct ttcgccgctt tgtctgcagc
    19501 gtcatcctgc gtcatgcggt gcagtttagg cgcggtcagc agcatcagta ctgcaataac
    19561 ggcagtagcg acaccaatct gcaagaatac gcgaccatag acttccagtg acatcagcgg
    19621 gtcggtaacg ttatccggca cagccatcat acccgcaaca taaccaccaa tcaggtttgc
    19681 accggcagtg gtcaggaacc agctacccat aatgaagccc atcagacgct gcggaacgag
    19741 ttgtgcaacc attgccagac ccagaccaga gatcatcagt tccccgatgc tctgcaggcc
    19801 atagcttgcg accagccagc tgacagacac gatacctgcg tcagacgcga atttcgcacc
    19861 cagcggcaga atcaggaacg caccagaaca catcaccatg ccgattgcaa acttggttgg
    19921 catcggcagg gtatcaccca tcttgttata gatagcggcc agaatcggac taccgatgat
    19981 gatccagaac gggttcagtg cctgatactg ctcaggttct acggccagac ccagaatgga
    20041 gtgctcaacg ttacgaatcg caaagaagtt cagtgacgtt ggcatctggc tgtacagcac
    20101 gaagaagatg attgcttcga gcatcaggat gaaggcaacg atcattttac gacgcgcagc
    20161 acctttcatc gcgaaagctt ctttaccgaa gataaccaca ataccgaagg caacaacgcc
    20221 cagcgccata cgcgcaactt cctgattgtg cagtagccag gtggcaacag cgatcagcgc
    20281 cacaacacca ataatggtca gcaacaggtt acggtagttg actggttcaa agtctggttt
    20341 tgaaccgtac tgtttaaccc agcgttggca gaaggcgaag ttaacgatag tgatcagcag
    20401 gcctacaacg ctcaacgtaa acgcaacact ccagccgtat ttcgcggcca gccacggggt
    20461 ggctatcata gagaagaaag agccgatgtt gacggacatg tagtacatgg tgaatgcacc
    20521 gtccagacgt gggtcgtttt tctcatagca tgtagaaagc agagaagacg ggttagcttt
    20581 aaacaggccg ttaccgaccg caatagccgc catacccata taaacaatac cggcgtcgtg
    20641 accagaccag gcaaccagag cataaccaat cgccagcaca atagcgccga gcataattac
    20701 gcgtttagta cccagtacct tgtcacctaa ccagccgccg atagcgacca gaccataaac
    20761 cagggcacta aaggaagaga aaagggtgat tgaatccgct tcagacatac ccagttgttt
    20821 aaccaggtaa acagccataa ttccttgtag gccgtaataa ccaaaacgtt cccataactc
    20881 aatcgagaag atgagataga acgccttcgg ttgtttgaaa gcgttcaaac tgacgctttc
    20941 agttggtttt tggtttgcag tggacacttt tacctctttt tttacatccc atattaacgg
    21001 gggtgctttc tcgcgttatg tctaaaaaac atacgcgcaa ttgttatagt gggaggggaa
    21061 acggcaggta atgttcacta tcttgcactt tcagacaagt cctttgtaat aatctgttac
    21121 atataactga ggcggggaat tcaaccattc cgggaaatgt tatccagcgt taaatttttc
    21181 aaaaaacgta acggcagatt ttttcactgc attgagaggg ggatgatggc ttgacggcga
    21241 tatgttctgc tatttagcgt ttttcctagt gagatagtcc agtttctgaa aaatagccag
    21301 tgtaatgttt tgcaggtcaa tatttgttgc gtgtgtaaat ttaaatcatt ttaacctgat
    21361 tcttgcaatg tttttacatt gccacagcat cgtgatcttg atcacgaatg aatagaaaat
    21421 ttcgctatta aaaaaacaat taacctgcat ttttggttat ccgcaaacgg attacctgat
    21481 gataacgcta agcaatggca tcattgacgt taaaaagatg ttttaaaaga ggataaagaa
    21541 aggttatcaa tggggtaatc gatgttaccc cttttcttca gatgtcaact ttctctttgt
    21601 attcacaaag atcttcaata atacaagagc cacagcgggg tttgcgggca atgcaggtat
    21661 aacgcccgtg caggatcaac caatggtggc agtcgacttt aaactctgcc ggaaccactt
    21721 tcagtagctt ttcttctacc tgttcgacgt ttttccccgg cgcaaattga gtacgattac
    21781 aaacgcggaa aatgtgcgtg tcgacagcaa tagtcggcca gccgaatgca gtgtttaata
    21841 cgacgttggc tgttttacga cctacgccgg gcagggcttc aagcgcagca cgatcttccg
    21901 gaacctcgcc attatgttgt tccagtaata tacggcaggt tttgatgata ttttctgctt
    21961 tgctgttata aaggccaatc gttttgatat aggttttcac cccttcaacg cccagttcaa
    22021 gcatcgctgc aggcgtattc gccaccgggt agagtttcgc cgtcgcctta ttaacactga
    22081 catcggtcgc ctgagcggaa agcagtacgg caatcagcaa ttcaaaaggc gaactgaaat
    22141 taagctcggt ggtgggatga ggattgttct cacgcaggcg agtgaggatc tccagacgtt
    22201 ttgctttgtt catcagacat tccctgtttc accgtttggc aatgcacgtt cggcagctgc
    22261 ttcagcacgg cgctttttca ttctttcatc aatcaggtat tttcctgcca gcatcagtcc
    22321 caagccaata aatgcacctg gtggtagcat cgccagcagg aaaggggagt cggtgtggaa
    22381 aatctccacg cgtaatactt ttgcccagct acctaacagc gcatctgcac cgtcaaacaa
    22441 cgtgccattg ccgataattt cgcgtagtga acccagcacg aacatggcgc aggttgcgcc
    22501 cgtaccaatt gaaaagccgt ccagtgccga aagcgccgga ccttttttgg cggcgaaggc
    22561 ttcagcgcgg cccacaacga tacagttagt gacaatcagc ggaataaaaa tccctaatga
    22621 ttgatacagg ccaaaggcgt aggcgttgat cagcatctgt acagcgctga ccaccgaggc
    22681 gatgatcatc acgtaaatgg gaatgcggat ctcggctggc gtccagtgac gcagcgtcga
    22741 aatggtcagg ttggtcagcg tcagtaccag cgtagtcgca agtcctaaac ccagagcgtt
    22801 agtggcagtg gacgtgaccg ccaatagcgg acaaaggccg agcagttgga ccagtgcaga
    22861 gttgtttttc cacaacccct gaacaataac gtctttaatt tcgctcacgc tttattctcc
    22921 acaggcagga agttgagaaa gttgtgccgg taacgtctga gcgtacaatc cggcgcgttt
    22981 taccgcatta accaccgcgc ggggagtaat cgtcgcgccg gtgaactggt cgaaatcacc
    23041 accatctttc ttcaccgccc agtgcgcatc atccgcacca ctgatttttt ttcccgcaaa
    23101 atgggtgatc cagtcagaaa ggcgcaattc gattttatcg ccaagccctg gcgtttcgtg
    23161 gtgctctgtc acgcgcgtgc caagtaccgt gccattaaaa tcggctccca ccagcagctg
    23221 aatcgcaccg gaatagccat ctggcgcggt tgcttccaga acggcggcta ccggtttgtc
    23281 atcctgtttg gcgatgtaaa cccgatgctc acctttacct aactctggcg cagttaccag
    23341 atagcaactc tgtgcgagcg cattgttata gcgttcggct ggcagcacct gatcaaataa
    23401 cgccttttgt tgcagactgg cctgttcagc aattgtcgtt ttggtcatct ggttgatggc
    23461 cgcagttaac cctgtagaac ccgctgcaaa tagtgccagt gtaatgccgt gttttcggat
    23521 agttttcagc atggtttacc ctttgcgatg gccgtagacg cgcggacgcg tgtagtaatc
    23581 gatcagagga accgtgatgt tcgccagcag gacggcaaaa gccacgccgt caggatagcc
    23641 gccgaaactg cggatcaacc agactaataa gcccgccagc gcgccgaaca tcagccgacc
    23701 acgattggtc gtagaagcag taactgggtc agtcaaaata aagaatgcgc cgagcatagt
    23761 cgcaccagac agcagatgaa tttgcggtgc tgccagtgtt tctggtgaga acaaccagcc
    23821 caacgttgcg cataacgcca gcgttactaa gaagctgagg ggaatatgcc agcgaatcgc
    23881 tttctgccat agcaaccaca cgccaccagc cagccaggcg agatttaccc attgccaacc
    23941 agcgcccgcc agaataccgc tgtagatcgg atattgcata atctgttcaa ccgaatgacc
    24001 tgcgcggaca gaggttttga atgtatccag cggtgtcgcc tgactaatgc catcaatacc
    24061 taagcgtagc gtattcatat caccaccact ggcggtatga ccgctgaaaa taacctgtat
    24121 ggcgtcgata aaaccaggga tgttgaccgc aatttcatgt ggcggtaacc agctggtcat
    24181 ctgcaccggg aaggagatca gtaagaccac ataaccaatc attgccggat taaacgggtt
    24241 ttgccccaga ccgccataca actgtttagc gatgatcacc gcaaacaccg tacccagtac
    24301 gaccatccac catggcgcta gggggggaat acttaccgcc agcaataagc ctgtcagcaa
    24361 tgctgagtta tctttcaacg ttgcggctac cgactgcttg cgtagtttga gtacgagagc
    24421 ttcggctaac agagcactga ccgacgccaa caggatctga acgagagtac cccaaccaaa
    24481 aaaccacagt tgcgctgcga ttcctggcac ggctgcgagc aacaccaaca gcataatgcg
    24541 cgatgtctgg cgctggttat gggtataagg ggagctagct attctgaata ccatttagtc
    24601 ctcgtttaca accttttgct gggcggcttt tttggcctga acgcgggcaa tagccgcggc
    24661 aactgcggct ttgcgcggat cgacctgttc ttctggttcc gcatt
//
LOCUS       DBKEKI010000055        24401 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.055, whole genome shotgun sequence.
ACCESSION   DBKEKI010000055 DBKEKI010000000
VERSION     DBKEKI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.055"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(23..1021)
                     /gene="fbp"
                     /locus_tag="ACUQPX_002162"
     CDS             complement(23..1021)
                     /gene="fbp"
                     /locus_tag="ACUQPX_002162"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HHV9056870.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1197..2570
                     /gene="mpl"
                     /locus_tag="ACUQPX_002163"
     CDS             1197..2570
                     /gene="mpl"
                     /locus_tag="ACUQPX_002163"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HHV9056871.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2653..3204)
                     /gene="yjgA"
                     /locus_tag="ACUQPX_002164"
     CDS             complement(2653..3204)
                     /gene="yjgA"
                     /locus_tag="ACUQPX_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HHV9056872.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3298..4650
                     /gene="pmbA"
                     /locus_tag="ACUQPX_002165"
     CDS             3298..4650
                     /gene="pmbA"
                     /locus_tag="ACUQPX_002165"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HHV9056873.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4833..5219
                     /gene="cybC"
                     /locus_tag="ACUQPX_002166"
     CDS             4833..5219
                     /gene="cybC"
                     /locus_tag="ACUQPX_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HHV9056874.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLADNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5264..5728)
                     /gene="nrdG"
                     /locus_tag="ACUQPX_002167"
     CDS             complement(5264..5728)
                     /gene="nrdG"
                     /locus_tag="ACUQPX_002167"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HHV9056875.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5886..8024)
                     /gene="nrdD"
                     /locus_tag="ACUQPX_002168"
     CDS             complement(5886..8024)
                     /gene="nrdD"
                     /locus_tag="ACUQPX_002168"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HHV9056876.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8418..10073)
                     /gene="treC"
                     /locus_tag="ACUQPX_002169"
     CDS             complement(8418..10073)
                     /gene="treC"
                     /locus_tag="ACUQPX_002169"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HHV9056877.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPCFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNGAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10123..11544)
                     /gene="treB"
                     /locus_tag="ACUQPX_002170"
                     /pseudo
     CDS             complement(10123..11544)
                     /gene="treB"
                     /locus_tag="ACUQPX_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11663..12610)
                     /gene="treR"
                     /locus_tag="ACUQPX_002171"
     CDS             complement(11663..12610)
                     /gene="treR"
                     /locus_tag="ACUQPX_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HHV9056878.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12795..12848
                     /gene="mgtL"
                     /locus_tag="ACUQPX_002172"
     CDS             12795..12848
                     /gene="mgtL"
                     /locus_tag="ACUQPX_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HHV9056879.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12989..15685
                     /gene="mgtA"
                     /locus_tag="ACUQPX_002173"
     CDS             12989..15685
                     /gene="mgtA"
                     /locus_tag="ACUQPX_002173"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HHV9056880.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKNLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15891..16277)
                     /gene="ridA"
                     /locus_tag="ACUQPX_002174"
     CDS             complement(15891..16277)
                     /gene="ridA"
                     /locus_tag="ACUQPX_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HHV9056881.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVALLPKDVKIEIEAIAVRR"
     gene            complement(16350..16811)
                     /gene="pyrI"
                     /locus_tag="ACUQPX_002175"
     CDS             complement(16350..16811)
                     /gene="pyrI"
                     /locus_tag="ACUQPX_002175"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HHV9056882.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16824..17759)
                     /gene="pyrB"
                     /locus_tag="ACUQPX_002176"
     CDS             complement(16824..17759)
                     /gene="pyrB"
                     /locus_tag="ACUQPX_002176"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HHV9056883.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17763..17897)
                     /gene="pyrL"
                     /locus_tag="ACUQPX_002177"
     CDS             complement(17763..17897)
                     /gene="pyrL"
                     /locus_tag="ACUQPX_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HHV9056884.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18178..18573)
                     /locus_tag="ACUQPX_002178"
     CDS             complement(18178..18573)
                     /locus_tag="ACUQPX_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HHV9056885.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18705..19418)
                     /gene="bdcA"
                     /locus_tag="ACUQPX_002179"
     CDS             complement(18705..19418)
                     /gene="bdcA"
                     /locus_tag="ACUQPX_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHV9056886.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19489..20082
                     /locus_tag="ACUQPX_002180"
     CDS             19489..20082
                     /locus_tag="ACUQPX_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHV9056887.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20227..20679
                     /gene="tabA"
                     /locus_tag="ACUQPX_002181"
     CDS             20227..20679
                     /gene="tabA"
                     /locus_tag="ACUQPX_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HHV9056888.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20802..22466
                     /locus_tag="ACUQPX_002182"
                     /pseudo
     CDS             20802..22466
                     /locus_tag="ACUQPX_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22519..23532)
                     /gene="argF"
                     /locus_tag="ACUQPX_002183"
     CDS             complement(22519..23532)
                     /gene="argF"
                     /locus_tag="ACUQPX_002183"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HHV9056889.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
     gene            23694..24110
                     /gene="rraB"
                     /locus_tag="ACUQPX_002184"
     CDS             23694..24110
                     /gene="rraB"
                     /locus_tag="ACUQPX_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HHV9056890.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(24156..>24401)
                     /locus_tag="ACUQPX_002185"
     CDS             complement(24156..>24401)
                     /locus_tag="ACUQPX_002185"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHV9056891.1"
                     /translation="ICELQKMYFLPAIRGKGLAKKLALMAMEQAREMGFKRCYLETTA
                     FLKEAIALYEHLGFEHIDYALGCTGHVDCEVRMLREL"
BASE COUNT         6053 a         5923 c         6290 g         6135 t
ORIGIN      
        1 ccgtgtggag aggcgcagga gattacgcgt ccgggaactc acggataaag cgttcgacat
       61 cttcaaccat atggtcgttg ccgacaaaga acgaacggcg ctggtgcaga gtttccggga
      121 tgatatccag aatacgctct ttgccatcgc tcgctttacc gcccgcttgt tccgccagga
      181 atgccatcgg gttgcactca taaagcaaac gcagtttgcc gtccgggtgg ctggcggtgc
      241 ttgggtagag ataaataccg cctttcagca ggttacggtg gaaatccgca accagtgaac
      301 cgatataacg cgaggtatac gggcgattgg tggatttatc ttcttcctgg cagaatttaa
      361 tgtacttctt caccccgttc ggaaacttaa tgtagtttcc ttcgttgatg gagtaggttt
      421 tgcctttctc cgggaagcgc atccgttcct ggcacaggca gaaaacgccc agcgaaggat
      481 cgtaagtaaa ggcgtgaaca ccgcatccgg tggtgtaaac cagcatggta gaggagccgt
      541 ataccacgta acctgccgca acctgtttgt taccaggctg gaggaaatct tcttccgtta
      601 ccggcgtgcc aacaggcgta acgcggcggt agatggagaa aatggtaccg acagagacgt
      661 taacatcgat gttggacgag ccatccaggg ggtccatcag caccacgtat tttgcgtgtt
      721 cacagccttc aaagacgaca atctcatctt cttcttcaga ggcaatcccc gcaacgatat
      781 cgcgtgcttt cagtgcggct ttcagttttt cattagcgaa caagtcgagt ttttgctgaa
      841 cctcgccctg cacgttctca gcaccgctgg cacccaggat atcaaccagt cctgctttgt
      901 tgatatcgcg atggataatc ttggcgccca gttttattgc cgacagcaaa gcagtgagct
      961 caccggtagc atgagaaaac tcgtgctgct tttcgacaat aaattcacct aacgttttca
     1021 taaaactttc cctgcaatgt ttatggagta aagcgaccgc aacaatctta acaaataatc
     1081 tcaatgttgc gctcaggtga atcgcgccag caaattgcgg attatcctga aatgcgtttc
     1141 tcacttgccc gacatatgcg taaaatgagc ggcagattaa aaaaggatag tgacgtatgc
     1201 gcattcatat tttaggaatt tgtggcacat ttatgggcgg tctggcgatg ctggcgcgcc
     1261 agttaggcca tgaagtaacg ggttcggacg ccaatgtgta tccgccgatg agcaccttac
     1321 ttgagaagca aggcattgag ctgattcagg gttacgatgc cagccagctc gagccgcagc
     1381 cggatctggt gattattggc aacgccatga cccgtggaaa tccgtgtgtg gaagcggtac
     1441 tggaaaaaaa catcccttat atgtcaggtc cacagtggct gcacgatttt gtgctgcgcg
     1501 accgctgggt gctggccgtt gccggtacac acggcaaaac caccaccgcg ggaatggcga
     1561 cctggattct ggaacagtgt ggttacaaac cgggatttgt gatcggcggt gtgccgggga
     1621 actttgaggt ttcggcgcgt ctgggcgaaa gcgacttctt tgttatcgaa gcggatgagt
     1681 acgactgcgc cttcttcgac aaacgctcta aatttgtcca ttactgcccg cgtacgctga
     1741 tcctcaacaa ccttgagttc gatcacgccg atatctttga cgacctgaaa gcgatccaga
     1801 aacagttcca ccatctggtg cgtatcgttc cggggcaggg ccgtattatc tggccggaaa
     1861 atgacatcaa cctgaaacag accatggcga tgggctgctg gagcgagcag gagctggtgg
     1921 gtgagcaggg tcactggcag gcgaaaaagc tgaccaccga tgcttccgaa tgggaagttt
     1981 tgctggatgg cgaaaaagtg ggcgaagtga aatggtcgct ggtaggcgaa cataatatgc
     2041 acaatggcct gatggcgatt gcagcggctc gccatgttgg tgtagcgccg gcagatgccg
     2101 ctaacgcgct gggttcgttt attaatgctc gtcgccgtct ggagttgcgt ggtgaagcga
     2161 atggcgtcac ggtatatgac gattttgccc atcacccgac ggcgattctg gcaacgctgg
     2221 cggcgctgcg tggcaaagtt ggtggtacgg cgcgcattat tgctgtgctg gaaccgcgct
     2281 cgaataccat gaaaatgggg atctgcaaag acgatctggc accttcatta ggtcgtgccg
     2341 atgaagtctt cctgctgcaa ccggcgcata ttccgtggca ggtggcagaa gtggcagaag
     2401 cctgcgttca gcctgcacac tggagtggcg atgtggatac gctggcagat atggtggtga
     2461 aaaccgctca gcctggcgac catattctgg tgatgagcaa cggcggtttt ggtgggatcc
     2521 atcagaaact gctggatggt ctggcgaaga aggcggaagc tgcgcagtaa ttcggcttca
     2581 gcctgatata gcattgccgg ataaggcgtt tacgccgcat ccggcaaacc atactcacat
     2641 caacaacgaa aattaccctt cattctccgc cagttcgcgc agatactgga aaatctggcg
     2701 tgcggatttc ggcggcttat tcccttcttt ctctttcttc gcgttgcgga tcagtgtacg
     2761 cagttgctga cgatcggcat cgggccacag attcaatact tcagcgatgg cgtcatcacc
     2821 ttgatcgatc agacgatcgc gcaggttttc cagtttatgg aacagcacca cctgctggtt
     2881 gtgacggttt ttcagcttat ccagcgcctg acgaataggc tctacgtcgc gctggcgcag
     2941 catcttaccg atgagctgca actggcggcg gcgaccttcc atcttaatac gctgggcgag
     3001 ctcaatagcc gggcgcagat ccgcatctaa cgggatctta tccagcgcgt ttttccccag
     3061 atcaacaatt tccgcgccaa ggcgttttag ctcctcggca tcacgtttaa tttcactttt
     3121 actgacccag ataatttcat cgtcttcgtc ttcgatgtca tcaccgggaa cgtcgtcgag
     3181 ccagtcttcg ggctgcttag tcatctcagg ctccttaaaa aaagaggcta atgttaccag
     3241 ttaagatgcg cactgaaaaa cggttctctg ttagacttca gagaaactct ctacattatg
     3301 gcacttgcaa tgaaagtaat ctctcaagtt gaagcgcagc gcaagattct ggaagaagca
     3361 gtttccactg cgctggagtt ggcctcaggc aaatcggacg gtgcggaagt tgccgtcagc
     3421 aagaccaccg gcattagcgt aagcacgcgt tatggtgaag tggagaatgt tgaattcaat
     3481 agtgatggcg cgctggggat cactgtttat caccagaacc gtaaaggtag cgcatcgtct
     3541 accgatttaa gcccgcaggc cattgcccgc accgtacagg cggcgctgga tattgcccgt
     3601 tatacctcgc cagatccctg tgccggcgtg gcagacaaag agctgctggc ctttgacgca
     3661 ccgaatctcg acttgttcca ccctgcggaa gtttccccgg atgaagccat tgaactggcg
     3721 gcccgcgcag aacaggcggc attgcaggcg gacaaacgca tcaccaatac cgaaggtggc
     3781 agctttaaca gccactacgg tgtcaaagtt tttggcaaca gccacggcat gttgcagggt
     3841 tactgctcaa cgcgtcattc gctctccagc tgtgtaattg ccgaagaaaa tggcgatatg
     3901 gagcgtgatt acgcctacac cattggccgc gcgatgagcg atctgcaaac gccagagtgg
     3961 gtcggggccg actgtgctcg ccgtacttta tcgcgcctgt caccgcgtaa actctccacc
     4021 atgaaagcgc cggtcatttt tgccaatgaa gtggcaaccg ggctttttgg ccatctggtg
     4081 ggggcgatag cgggtggctc ggtttaccgt aaatctacct ttctgctgga ttcgctgggt
     4141 aaacaaattc tgccggactg gctgaccatt gaagagcatc cgcatctgct gaaagggctg
     4201 gcgtcgacgc cattcgacag cgaaggtgtg cgcaccgagc gtcgcgatat tattaaagat
     4261 ggcatcctga ctcagtggct gctgaccagc tactcggcgc ggaaactggg gctgaaaagc
     4321 accggacatg cgggcggtat tcacaactgg cggattgccg gacaagggct aagcttcgaa
     4381 caaatgctca aagagatggg caccgggttg gtggtgacgg agctgatggg ccagggcgtg
     4441 agtgccatta ccggtgatta ttcccgtggt gctgcgggct tctgggtaga gaatggcgaa
     4501 attcagtatc ccgttagtga aatcaccatc gcaggtaatt taaaagatat gtggcgcaat
     4561 attgtcaccg tcggtaacga tattgaaaca cgcagtaata tacagtgtgg ttctgtgctg
     4621 ttgccggaga tgaaaatcgc cgggcagtaa aaaactggcg cgacctgccg cgctataaac
     4681 taaaaaatta cacaaaatca ttcgcactgc atcgagacgg aaagtgaatg agggccgggg
     4741 agcgtactcg cagtacgtga cccgggcgaa tgagcgtagc cgtcgatgag gcagtgtgaa
     4801 ggatgaagtg taaataaaaa aggaagtgag caatgcgtaa aagcctgtta gctattcttg
     4861 cagtctcctc gttggtattc agttctgcgt cgtttgccgc tgatcttgca gacaatatgg
     4921 aaaccctcaa cgacaattta aaagtgatcg aaaaagcgga taacgcggcg caagtcaaag
     4981 acgcgttaac gaagatgcgc gccgcagcgc tggatgcgca aaaagcaacg ccgccgaagc
     5041 tcgaagataa atcaccggac agcccggaaa tgaaagattt ccgccacggt ttcgacattc
     5101 tggtcggtca gattgacgac gcgctgaagc tggcaaatga aggtaaagta aaagaagcgc
     5161 aggctgctgc agagcaactg aaaacgaccc gcaacgccta tcaccagaag tatcgttaat
     5221 tcctcatttc cctgttgcct gcactcaggt aacagggaac ttatcatcgc aaatgatgca
     5281 ccacctgatt gctgctgccg cgccagatca gggaagggtc ttttaaatcc tgcacaaatt
     5341 tgccgtcgac cagcacgtta atcaaatcaa caacctgcat ttgcgcagcg ttgagttcgt
     5401 cgagtttata gcctgtccac acccagatgt ctttacccgg acactcggcg cggatgcgtt
     5461 ttaccagttt cagaatatcc ggcacgtttt gcggatgcag cggatcgccg ccggagaggg
     5521 aaatcccctg gcgtttgata cgagtgtcat tcagatcttt aatgatcttg tcttccattg
     5581 ctttggtaaa tggctgacca gaatttaccc gccatgtgct tttgttatag caaccggggc
     5641 attcatgaac acacccggag acaaacaggg tgcagcgagt gccggggccg ttgacgatgt
     5701 cgacaggata gtactgatga taattcatta tttcgcctgg cttatcagtc cgcgtgtagg
     5761 ctgcatccgg taatcaatgc ctgatgcgac gctgtcgcgt cttatcaggc ctacagccgt
     5821 tgccaaatgt aggccggata aggcgtttac gccgcatccg gcatttgctt agcgcagagt
     5881 gaagattaac ctatctgccc attccccaaa tgtttaacgc ggcgcttaac ttcttcctgc
     5941 ttaccagcgt taaacggacg tgcatccggg ctacctaaat atccgcacac gcggcgagtt
     6001 accgacacac gggaggcgtc atggttacca catttcgggc aagtgaagcc tttgctggtg
     6061 cactcgaact caccggtaaa gccacactcg tagcactcat caatcggtgt attggtgccg
     6121 taatacggta catgctgata gctgtaatcc cagacatctt ccagcgcttt caggttgtgc
     6181 tgaatgtttg gatactcgcc gtagcaaatg aaaccaccgt tcgccagcgg cgggtaaggc
     6241 gcttcaaagt cgatcttgtc gtacgggttc accttcttct ccacatcaag gtggaaactg
     6301 ttggtgtagt aacctttatc ggtcacgccc ggcaccacgc caaactcagc agtatcgaga
     6361 cggcagaagc ggtcgcacag gttttcactc ggcgtgctgt agagactgaa accataaccc
     6421 gtttcttctt tccactgatc cactgcctga cgcagacgtt caacaatggc gatacctttc
     6481 gcgcgaagct tctcgttgtc gtaaacatgc tcgccgccga acagcgcgtt aatggtttcg
     6541 tggatgccga tgtaacccag cgaaatagac gcacgaccgt ttttgaagat ttcagaaaca
     6601 tcatcgtcag cattcagacg tacgccacaa gcaccttcca tatagaggat cggggcgacg
     6661 cgcgctttca cgccttcgag acgagcgata cgggtcatca gtgccttacg tgccagcacc
     6721 agacgttcat ccagcagctt ccagaaggtg gcttcatcgc cttttgcttc cagagcaata
     6781 cgcggcaggt tcaggctgat cacgccgagg ttgttacgac catcgtggat ctgctcgccg
     6841 ttttcatttt cccacacgcc gaggaagctg cggcagccca tcggggtttt aaacgaaccg
     6901 gtgactttca ctacctgatc gtagttcagg atatccggat acatgcgctt gcttgcgcac
     6961 tccagcgcca gctgtttgat gtcgtagttc ggatcgcctt ttttatggtt caggccatcg
     7021 cgaatcgcaa acaccagttt cgggaacacc gcagttttac ggtttttacc gaggcctgcg
     7081 atacggttac gcaggatcga ttcctgaatc aggcgcgatt cccagctggt gcccagacca
     7141 aaaccaaagg ttacaaacgg cgtctgaccg ttggcggtgt gcagggtgtt tacttcgtac
     7201 tccagcgact ggaaggcatc gtagcactct ttgatggtac gagagttagc gtagccttcg
     7261 gcgtccggga tgttccactc ttctgcggtc ttgcgatgtt tgttgtagct ggcggtaaca
     7321 aacggcgcca gcacttcatc gatacggtta atggtggtgc cgccataaat atggctggcg
     7381 acctgagcaa taatctgcgc agttaccgcg gttgccgtag agatcgactt cggcggttca
     7441 atctcggcgt tccccatttt aaagccctgg gtcagcatgc ctttcaggtc gatcaacatg
     7501 cagttgaaca tcgggaagaa cggcgagtaa tcgagatcgt gatagtgaat atcgccacgc
     7561 tcatgtgcct gcaccacgtc acgcggcagc aggtgctggc gtgcatagtg tttagccacg
     7621 atcccggcca gcaggtcgcg ctgggttgga atcaccttgc tgtctttgtt ggcgttttcg
     7681 ttgagtaacg aggcgttggt ctgctcgacc agaccacgga tctcctggtt caggcgaccg
     7741 cgtttttcac gttcaatgtc gcgatcgtga cggtactcga tgtaagcacg agccagttgt
     7801 ttgtatggac ccgacatcag ctggttttca actgcggtct ggatctcgtt gatatccacc
     7861 tggttgcggc cctgcatctg ctcgctgaca accgcggcaa cagtggcgca ataatcggca
     7921 tcatcgactt ccgctgcttt agctgcacgc agaatcgctt ctttgatgcg ctctgattta
     7981 aacggcactt tgcagccgtc tcgtttcatc acatgcggtg tcatgatcgc tccatatttt
     8041 taagaacagg ttatccacag aaattgggaa aggcgttttc cggttttttc gtttcgtttt
     8101 ccggtgcttt ccgcaatcgc catccgctta tccacaacgc cgcacctttt tcgtggggag
     8161 ctgtagtagc aattatagtc gattgataca acatattggg ttgggacgca ttttaaagtc
     8221 tatatatagt gctttgcatc aaggatgttt taactttttt tgatgtagct caaagtaaaa
     8281 agcagagcgt acggatgacg ggcgctacag cgttatgtaa attttttaat gaatttgctg
     8341 gttgaaaaat caacaaaaac aacatgctga cagacaaaac cccggaatga ccggcattat
     8401 cggggcttag ggaagattta cttctgtaac caccagacag cctcaaaagg ccgtaaattc
     8461 atggcacagg gttgtggtga ggcttcttcg tagttatgca tcacaagctg ccagttgccg
     8521 cgcatttgcc ctggctgcca gggttggatc tcacggctaa ggttggcaat gaccagcaag
     8581 gtttgcccct tccattcacg gcgatagcac cacaatacag ggctgtttgg cagcagatct
     8641 tggtaattgc cccatgtcag gatggcttct tgcttacgca gtgcgattaa cttttggtag
     8701 gtgtaaaaca cagaggagtc atcggccagc gcggcttcta cgttgatttg ttgatagtta
     8761 tcgcctaggc caatccacgg ttcgccagcc gtaaacccgg cattatcgcc gttgctccat
     8821 tgcatgggcg tgcggctgtt gtcacgggat ttactggcga ggattgccaa taactcgtcg
     8881 gcatcacgac catcgttgcg cagctcggca aacatattga ggctctccac gtcgcgatag
     8941 tcagtaatgc gcgtgaaatg cgggttggtc atgccaatct cttcgccctg gtagatatac
     9001 ggcgttccct gcatgccatg cagcaccatc gccagcattt ttgccgcagg cacgcggtat
     9061 tcaccttcat caccaaagcg agaaacaatg cgcggctgat cgtggttaca ccagaacaag
     9121 gcattccatg ccccgttgtg cattccttgt tgccagtggc ggaacaatgt tttcaacgcc
     9181 acaaagtcag gtttagccag cgtccatttt tcaccaccgg gataatcgac cttcaggtga
     9241 tgaaaattaa aggtcatcga caattcactg cctgtcagtg ccgcgtatcg ctggcaatgc
     9301 tcaaggctgg tggaggacat ttcacctacg gtcattaacc cgcgtggcgt aaacacatcg
     9361 cggttcatct cgtgcaaaaa ctcgtgtgct cgtggcccgt cggtgtagaa gcgacgcccg
     9421 tcgccatcca ggtcttcagg gaaacacggg tctttggaga tcagattcac cacatccagg
     9481 cgcaacccgt cgaccccacg atcggcccag aattcacaga cttttttcag ctctgcgcgt
     9541 accgctggat tctcccagtt gagatccgcc tgttctggtg caaagagatg caaataatac
     9601 tgttcgcttt ccgcatgcca gcgccacgca ctaccgccaa attttgaacg ccagttgttc
     9661 ggtggcgttt ctggttcgcc atcgcgccag atataaaact ggcggtaagg gctttctttg
     9721 ttcagcgcct cgcgaaacca ggcatgttgg gtagaggtat ggttaaacac catatcgaga
     9781 atgatacgaa tcccgcgcga ttttgcctgc gtcaccagtt cgtcaaaatc gtccagcgtg
     9841 ccgtaggtgg gatcaatcgc cgtatagttc gctacatcgt aaccgttatc gacctgggga
     9901 gagacataaa agggggttag ccagatggca tcaacgccca gtttatgcag atagtccagg
     9961 cgttggataa cgccacgtaa atcgccggtg ccgctacctg tggtgtcctg aaaactcttt
    10021 ggataaatct ggtagataac gccgttttgc caccagtggg gaagattagt cattacgtta
    10081 ttcctgcaaa tgcgaggggg cgcaattgcg ccccgaagaa aattaaacaa tgtccagcgt
    10141 gcccaggcgg tatttccgct gatagataaa cgaggtgagt acaatcggga tgatgatggc
    10201 gatagccatt gccagcgcaa atacctgcca gtagctcggt tgaatcgaga gaattcccgg
    10261 caggccccct acgccgatac cattcgccat aacgccgttc aggccgcata gcaatcctgc
    10321 cagaccagaa ccaatcatcg cgcacagcat cgggaagcga tatttcaggt tgatgccgta
    10381 cattgcaggc tcagtgaccc caagccaggc ggagatagcg gcaggcacgg agatctcgcg
    10441 ttcattgtgc ttgcggctgg aaatgataat gcctatcacg gcggagccct gagcgatatt
    10501 cgacagcgca atcagcggcc acactggcgt gccacccatg ctttgaatca tctgcaaatc
    10561 aatagcaagg gtggtctggt gtacgccggt gatcaccagc ggcgcgtaca ggaagccaaa
    10621 caatgccgca ccaatcggag caaagctgcc ggtcatcagg tgacgtaccg caaaggcaac
    10681 gccatcgcca atcatgcgac caaacggacc aatcagcgca tgggcgagga acaccgcgag
    10741 gatcagcgaa cagacgggca ccaccaccag atagaggtaa tccggtacga tgcgtttaag
    10801 gcgagtttca ataacgccca gcgccagccc ggctaacagt gccgggatca cctgcgcctg
    10861 atagcccact ttggcgatgc tgaacatgcc aaagtcccac acttccggca gctgctgccc
    10921 gagcagataa gcgttcatca gctgggggga aaccagtgtc acgccaagca cgataccaag
    10981 gatcggcgtg ccgcccattt ttttcaccgc tgaccagcaa atcccgaccg gcaggtagaa
    11041 gaaaatcgct tcaccgatca accacagaaa atcgtagatc gtttgcaggg aagggtacat
    11101 ttgcgccagc gtctgaccgt tgctcatggg caaatcgccg atcacattgc gaaaaccgag
    11161 gatcaaaccg ccgctaatca acgcgggcag caacgggaag aagatctccg cgaaatgaga
    11221 gatcaactgc tcatgccatt tcatattttg ccgggcggct ttttttacct gctctttatc
    11281 aacctgcgcc tgtccggttg acgctattag tgcttaatag taatcaccca cgttggtgcc
    11341 aatcaccacc tgaaattgcc cggcattggt gaaacagcct ttcaccatgg ggagttgctc
    11401 aatttctttc ggtctggcat tggccggttg gttgaggaca aagcgcaggc gagtaataca
    11461 gtggctcacc gtcgcaatat tgccgcgccc gccgaccagt tcaatcaacc gatcgatatc
    11521 cgtttggttt attttgctca tcataaagcc ccatggcaga tgacattttt ggttggctgc
    11581 agaatatatt gcgcggaaaa atttaaaaac gggaacgttc ccgaaacgca gcgaagatca
    11641 caatttatcg ttcaggaaac gatcaggaca gggtggcggg gatgatgatt tgttgcggtt
    11701 cgctgcgccc ggttacctgt gcgatcaatt ggcaggccgc ctggcgtcca gcttcggcgt
    11761 agccaggatc tacggtgacg atctccggat gaaggaattt cattaacggc gtattaccta
    11821 cgctcgccag ttgcaaggtg tcgatgcgtt gctcttgcag gtatttactt gcgccaagag
    11881 ccagcgtgtc ggttgcgcac agtaacgcgg tagtttcagg cgtaatcact tttgctacgt
    11941 tttcatagcc ttgcttcata gcaagccctg gcagagcggc aacgggatgc agtttatgcg
    12001 ctttgcagaa cgccaggtag gcttcgtgac gtcgcttccc ggtcgtcacg tcactatgcg
    12061 gcacgccgag ataactgata ttgcgatgcc cctggtcata gagccgttgc atcagaatct
    12121 tgattgcccc ttcgtcgtca taacagaccg aagcaaagcc ttttgcgtca cgcgccagca
    12181 gaaccagcga tgactgccag tgggctaaca tttcttcggt tatgccggta aaaccgaaca
    12241 gcactacgcc gtcgatatta cgccgtttca gcacccccaa atgttcggca actaattgtg
    12301 gggaaaactg attttccatc atgattggat cgtaaccttg ttcatagaac gctggcagca
    12361 tggtttgaac ggcgagattt tctgacaacg aatccagacg ggtaacaatg atggcgacca
    12421 ctttatcgct ttgcccacgc atagcgcgcg cagagcggga aggggaaaat ccatgctgat
    12481 tcatcactgc ttcaacacgc tcgcgcgtgc gctgactcac gccgctttcg ttattcagca
    12541 cccgggaaac cgtagatttc cccacgccgc ttaagcgcgc gatgtctttg atggtcagcc
    12601 gattttgcat cctgttgtcc cataacgtgt tgtttaatta tttgagccta actttacccg
    12661 tgcattcagc aatgggtaaa gtctggttta tcgttggttt agttgccagc aggtattata
    12721 tcgccataaa tgctacgaat attattggat tctccttatt atttgcggcg cttttttcac
    12781 ttaccggagg ttatatggaa cctgatccca cgcctctccc tcgacggaga ttaaaacttt
    12841 tccggtaagc ccgtcttttc acggcgttac cggatgcgta aggccgtgac gttttaatgt
    12901 ccctgctcag ctttattacc ttcaggtaag gcttcgccac gcctgaagac atttctgtac
    12961 tgtttcagac agtgcggagg gactccttat gtttaaagaa atttttaccc ggttcattcg
    13021 ccatttacct tcccgtctgg ttcatcgcga tccattgcct ggcgcgcagc agacagtgaa
    13081 tacggcggtc ccgccgtcct taagtgcgca ttgcctgaaa atggcggtga tgcccgaaga
    13141 agaattgtgg aaaacgttcg acacccatcc ggaaggatta aatcaggcgg aagtggaatc
    13201 tgcccgcgaa caacatggtg ataataaatt acccgcacaa caaccgtcgc cgtggtgggt
    13261 acatttatgg gtctgctatc gcaacccctt taatattttg ctcaccattc ttggtgctat
    13321 ttcttacgcc acggaagatt tatttgccgc aggtgttatc gcgctaatgg tcgctatttc
    13381 tacgttgctg aactttattc aggaagctcg ttccaccaaa gcggcagatg ccctgaaagc
    13441 gatggtcagt aataccgcga cggtactgcg agtaattaac gacaaaggcg aaaatggctg
    13501 gctggagatc ccgatagacc agctggtgcc cggcgatatt ataaaactgg cggcggggga
    13561 tatgatcccg gcagatttac gtatcttgca ggcgcgggat ctgttcgtcg ctcaggcgtc
    13621 gttaaccggt gagtctctgc ccgtggaaaa agccgctact actcgccagc cggagcacag
    13681 caatccgctg gagtgcgaca ccctgtgttt tatgggcacc accgtggtga gcggcacggc
    13741 acaagcaatg gtgattgcta caggagccaa cacctggttt ggtcaactgg cggggcgtgt
    13801 tagtgagcag gaaagcgagc cgaatgcctt tcagcaaggg atcagccgcg ttagtatgct
    13861 gctgattcgc tttatgctgg tgatggctcc ggtggtgttg cttatcaacg gttacaccaa
    13921 aggcgactgg tgggaagcgg cactgtttgc gctttcggtc gcggtaggct taacgccgga
    13981 aatgttgccg atgattgtta cctcgacgct ggcgcgcggc gcagtaaagc tgtcgaaaca
    14041 gaaagtgatc gtcaaacatc tggatgccat tcagaacttt ggcgcaatgg atattctgtg
    14101 cactgataaa accggcaccc tgacgcagga caaaattgtg ctggagaatc ataccgatat
    14161 ctccggtaaa accagcgaac gcgtgctgca tagcgcgtgg ttgaacagtc attaccagac
    14221 cggacttaaa aatctgcttg ataccgcggt gctcgaaggt acggatgaag agtcagcgcg
    14281 ttcgctggcc agccgttggc aaaaaattga tgagattccg tttgatttcg agcgtcgtcg
    14341 gatgtcggtg gtagtggcag aaaataccga acaccatcag ctggtttgca aaggtgcatt
    14401 gcaggaaatc ctcaatgtgt gttcgcaggt gcgtcacaat ggcgagattg taccgctcga
    14461 tgacaccatg ctgcgtaaga ttaagcgggt tactgatacg ctgaatcgtc aggggctgcg
    14521 cgtggttgcg gtggcgacga aatacctgcc agcgcgtgaa ggagattacc agcgggcgga
    14581 tgaatccgac ctgatcctcg aaggatatat tgctttcctt gatccgccaa aagagacaac
    14641 cgctccggca ctgaaggcat taaaagcgag tggaattacc gtaaaaatcc ttactggcga
    14701 cagtgagtta gttgcagcga aagtgtgcca tgaagtgggg ctggatgcgg gagaggtggt
    14761 cattggtagt gatattgaaa cgctatctga cgatgaactg gcaaatctcg cacagcgcac
    14821 cacgctgttt gcccgcctga cgccgatgca taaagaacgc attgtgacct tactgaagcg
    14881 cgaagggcat gtggttggct ttatgggcga tggtattaat gatgcgcccg ccttacgcgc
    14941 tgcggatatc ggcatttctg tggacggcgc ggtagatatt gcccgtgaag cggctgatat
    15001 catcctgctg gaaaaaaacc tgatggtgct ggaagagggg gttattgagg gacgtcgcac
    15061 tttcgccaac atgctgaaat acatcaaaat gacggcgagt tctaacttcg gtaatgtgtt
    15121 cagcgtgctg gtggcgagtg ctttcttgcc cttcctgccg atgttgccgt tacacttgct
    15181 tattcagaac ctgctgtacg atgtgtcaca ggtggcgatc ccgtttgata acgtcgacga
    15241 cgagcaaatt caaaagccgc agcgttggaa tccggcggat ctggggcgct ttatggtctt
    15301 cttcggaccg atcagctcga ttttcgatat tctgacgttt tgcttgatgt ggtgggtatt
    15361 ccatgccaac acgccggaaa cgcaaacgct gttccagtcg ggctggtttg tggtgggatt
    15421 actgtcgcaa acgctgattg tgcatatgat ccgtacccgc cgtgtgccgt ttattcagag
    15481 ctgtgcatcg tggccgttaa tgatcatgac cgtgatcgtg atgattgtcg ggatcgcatt
    15541 gccgttttca ccgctggcca gttatctgca gctgcaggcg ctgccgttaa gctatttccc
    15601 gtggctggtt gcgattctgg cagggtatat gacgttaacc cagttggtga aagggttcta
    15661 tagccgtcgt tacggctggc agtaaagaat aaactgggca tggtggcccg gattcagatt
    15721 gcagacaaag tgagctttgt tcatgccgga tgcggcgtaa acgcccaatc cggcctacaa
    15781 attcaataaa ttgcaggaat cacgtaggcc tgataagcgt agcgcatcgg gcgattttgc
    15841 ttttgtcatc agacttaatc cgggcatgat agcccggatt tccatcaaga ttagcgacga
    15901 acagcgattg cttcgatctc aatcttcacg tctttcggca gaagggcaac ttcaacgcaa
    15961 gaacgtgccg ggaaagtggc gttgtgttcg gtgaagaagg cttcgtaagt ggcgtttacg
    16021 gttgcgaagt cgttcagatc tttcacgaac acagtggttt taacgatgtc gcccactttc
    16081 aggccagcgg cttcgacgat tgcttttacg ttatccagcg actgacgtgc ctgtgcagcg
    16141 acgtctgccg gtacttcacc agttttcgga tttaccggga tctgaccgga ggtgatgatc
    16201 atattgccca gatcaacgcc ctgtacgtaa ggaccgatag ctgccggtgc attttccgtc
    16261 gcgatagttt tgctcatgat ttctccttta ttacagcggt aaaaagtctg gctcattata
    16321 gggagccaga cttttattac caaccgcaat taattggcca gcaccacatt atgggaaaac
    16381 tctttttcac agtatttgca tttaagcgcg atgtcattgg cgcgttttcg cacggcaaag
    16441 ctggatgaaa ccggttcggc atggctgata cagttgctgt tcgggcagac cagcacattg
    16501 tcgatgcgct ccggcagact tgggcgcgat ttacccacca cttcatagtt gtcgatacgg
    16561 ttaaccgtgg cttgcggtgc atacaatgcc agttggtcta cttgatcttc actcaaaaag
    16621 gtattttcga ttttgatcag atctttgcgg cccatctcgc cagaaggcaa attcagacca
    16681 atggtgatgc gctgatctgt ttcggtcaac ttgaacagac tcaacagctt aaaaccgatc
    16741 tgggcgggga tatggtcaat taccgtgccg cgtttaatag cttcaacctg caatttatta
    16801 tcgtgtgtca tctctatttc cccttacagt accagatcgc gattcagaac cagtgccagt
    16861 aacgcctggc gagcgaaaat tccgttgcct gcttgctgga agtaccaggc gtgaggcgtt
    16921 ttatccacat ccgtcgcaat ctcatcaaca cgcggcagcg gatgcagcac tttcatattg
    16981 gctttggcgt tgtggagatc gctggcgcga agaacaaact gcgctttcac gttggcgtac
    17041 tcggacgggt ccagacgctc tttttgcacg cgggtcatgt acaggatgtc tacttccgcc
    17101 atcacttctt caatagagct gtgcagactc caggcgatcc ctttttcatc gagcatatcc
    17161 agaatgtatt gcggcattgc cagcgcatcc ggcgcgataa agtaaaaacg gttgccgtcg
    17221 aacttcgcta gcgcctgggt cagggagtga acggtgcggc catatttcag gtcaccaacc
    17281 attgcgacgt ggagattgtc cagacgcccc tgggtttcct gaatggtgaa taagtccagc
    17341 aaggtttgcg tcggatgttg gttggagcca tcaccggcat tcagtaccgg tacattgcca
    17401 gaaaattcgg tggccaggcg cgccgcacct tcctgcggat gacgcatcac tatcgcatcg
    17461 acgtaagtgc tgataaccga aatggtatcg gccagcgttt cgcctttttt acccagtgat
    17521 gtattggcgc tgtcggagaa gcccaccacg ctggcaccca ggcggtgcat ggaagtttcg
    17581 aaagagaggc gggtacgggt agaggcttcg aagaaacagc tggcaatgac tttgtgcttc
    17641 aacagctctg gttgcgggtt tgctttcagt ttcgccgctg tcgccagcac cagattaagg
    17701 tcatcgcgac taaggtcgtt tatggaaatg atatgtttct gatatagcgg attagccatc
    17761 ttttatctcc tgacgcctag gcaaaaaaaa gcccctcgat tgaggggctg ggaatgggtg
    17821 atcaacggga agaaaaacgg caggccagcg tcttttttca gacgcggtaa gacaaaatgt
    17881 cgaacacact gaaccataca tcctcccggc aaattgtccg gcattatact catagccaga
    17941 agcggtgcaa gcatttgatg caatattttg tcagcgcaaa cggtttattt gaattaaaag
    18001 tcaaaatata tgcattttta ttttctatga ttctggaggg gaagtgtcgt tcggtatcaa
    18061 taattgcaga catttcctgc ttttttcttt ttcattttca cgcaatcaga aatgcatttt
    18121 attccattca tccgacttat agggcggagg gtgaaaacga acggctaaca ctattgctta
    18181 tagttcaggg atacgcgcta tcactttaat ttcaaaatca aagcctgcca gccaggtaac
    18241 acccaccgcc gtccagtttg gataaggtgg ggcgctaaat atttcatttt tcaccgtcat
    18301 gatgtcttca aattgttttt ccggatcggt atgaaagctc gtaacatcaa tgatatcgtc
    18361 aaaagtgcat cccgcagctg ccagggtcgc atgcaaatta tcaaaggcca gtctgacttg
    18421 ttgctgaaaa tcgggttctg gtgttccgtc ctctcgactt cctacttgcc cggaaacaaa
    18481 cagcaaatcg ccggaacgaa tagccgcaga ataacgatgc tcagcataca gtgaatgtcg
    18541 gccagcaggg aaaacagcgg ttctttctac catttggtta tcctcaagat ttacgacatg
    18601 aacagaagat ttatatttac cgggagccgc ttttaggggg acgacgttag taaacaaaac
    18661 ccagacatca tggataatgg ctgggcttaa ttgagcgtag tcggttatgc gccaaacgcg
    18721 ccatcaatgg tatgcatcgc gccggtaaca aaactggctt ctggccctgc taaccatgcg
    18781 accataccag cgacctcttc cggttgccca tgtcttttga tggccataaa actatgcaac
    18841 atatcgcgca ttggcccgtt ggcgggatta acgtcggtat caattggtcc tggctggacg
    18901 acgttgatgg tgatccctcg cggtccaaaa tcacgggcca gcccgcgcgc catgccttgc
    18961 agggcagatt tactggcggc ataagcagcc atgcctgcaa caggcatacg atcgccattc
    19021 acggagccga tgattaagat gcgcccgcct tcgggcatct gccgggcggc ttcaacagag
    19081 gcatgataag gagcatgaat attgattttg aaaaggcgat caatatcgtc ggcatttaat
    19141 tccagggcat cgccaaagac gccaatacct gcatttacca ccaggatatc caatgcgccg
    19201 ctcttacgaa cgacatcaat aacagcgtct ctgtcagcac tatctgtgaa tactgctgtc
    19261 gctccagtct cttgtgccag acgttcagcg gcatctttcg accccgcata ggtgaatcgt
    19321 acattggccc catcggtgac gaaacgacgt acgatagcgg caccgattcc acgactgcca
    19381 ccgaggatga gaactgtctt accggtaaaa gcgcccataa ggactccttg atttattatg
    19441 taacatgcat tacaaaactg ttttaacttt ctgtcaacaa gttttgtaat ggtcactaaa
    19501 aaacaatctc gtgttccagg tcgtcccaga cgtttcgctc ctgagcaggc agtctctgcg
    19561 gcaaaagtgc tttttcacca aaaaggtttc gatgcagtaa gtgtctctga agttactgat
    19621 tatcttggta ttaacccccc gagcctctac gcggcttttg gcagtaaggc tgggttattt
    19681 agccgtgtac tcaatgaata tgtcggtacg gaagctattc cgcttgccga tattcttcgt
    19741 gatgatcgtc cggtaggcga gtgcctggtt gaggtattaa aagaagcggc gcgcagatat
    19801 agccaaaacg gcggctgcgc tggctgtatg gttcttgaag gtattcatag tcatgatcca
    19861 caagcgcgtg atattgccat tcaatattat cacgccgcag aaacgaccat ttatgactat
    19921 atcgccaggc ggcatccaca aagcgcacaa tgtgtgactg attttatgag taccgtgatg
    19981 tcagggcttt ctgcgaaggc acgggagggg cactccatcg aacaactctg tgcaacagct
    20041 gccctggcgg gggaagcgat aaaaactctt ctcaaggagt gatgctggcc ttgatccgaa
    20101 aggcgggaac gcgcctgccg ataagttgtg ataagacaat aattcacgcg ttaaggctgg
    20161 cggaattgat catcttttcg tataacgata gaaatgaaac attgttttaa ttaaggagtg
    20221 gaaaagatga tcatcggaaa tattcataat cttcagccgt ggctacccca ggagttacgc
    20281 caggcgattg agcatatcaa agcacacgtt acggcagaaa cgccaaaggg caagcacgat
    20341 atcgaaggca atcgtctgtt ttatcttatc tcggaagata tgaccgagcc gtacgaagct
    20401 cgccgtgcgg agtaccatgc ccgctatctt gacattcaga ttgtgttaaa aggtcaggaa
    20461 ggcatgacct tcagcacgca acctgcaggc gcgccggata ccgactggtt agctgataaa
    20521 gacatcgcat ttttgccgga aggcgttgat gagaaaacag ttatcctaaa tgaaggtgat
    20581 tttgttgtgt tttatccggg ggaagtgcat aaaccgctgt gcgcagtggg cgcgccagcc
    20641 caggttcgca aagcagtagt gaagatgctg atggcgtgat gacttttcgc cgtaaataac
    20701 tccaggttta cggcgagttt gtgaaaagag cgttttttga tatttttttg tgagtaaaat
    20761 ttgtaatgct tagacgttct tattaactca aggagttcgt catgagcaaa atatcagatt
    20821 tgaattattc tcaacacatt acattagccg acaattttaa acaaaaaaat gaagccttag
    20881 atacctggta tgtgggaatg aatgattttg cccgaattgc cggggggcag aatagcagaa
    20941 gcaatattct ttctcctaga gcatttttgg agtttttggc aaagatattt accctgggtt
    21001 atgtggattt tagcaaacgc tccaacgaag cgggtagaaa tatgatggct catattgagt
    21061 tctcatctta ttctaaagat actgatggca atgaaaaaat gaagttttac atgaataatc
    21121 ctgaagggga gcgagcggat ttatctaagg tgaagacaga aattacactt gcctcagctt
    21181 ctactaaagg gattcgtgaa ggacatacag tcattatatt taaacaatct gatggttcga
    21241 ctaatcgtta tgaagggaag tcttttgaaa gaaaagatga tagttcatta cacctgatta
    21301 ctaacaaggt tctggcgtgt taccaacgcg aagctaacaa gaaaatagcg cgtctattaa
    21361 ataatcatca gaagttaaat aatctacaga agttaaataa tatacaggag ttaaatgatt
    21421 ctcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct acgattacgg
    21481 atttattaga aaaagcattg aataaagcct tattagcaat aaggaacgaa catctgctat
    21541 taatgcctca tgtatgtgat gaatagattt catacttact gggcgaaaat ggtatacttg
    21601 aagaaataga taagctctac gaattaaatg atcacggaat tgataatgac aaagaaggta
    21661 acaatgaaat taatgacatc atgattaacc tgtctcatat tcttattgat tccttagatg
    21721 atgcaaaggt taaccttaca ccggtcatcc attcgatgtt ggtgactttt ttagaattgc
    21781 catataataa tgatgtaaaa atactggagt ggtgttttaa taaaagcatg caatattttg
    21841 atgattctgc aaagatagag catgcatgct ccgtaataaa tcatattaat tttctctgcg
    21901 atcagtctaa agtagctgag acattatttt tcaatctcga taaagaaccc tataaaaata
    21961 gccctgaatt acaggagttg atttggaaaa agttggttgt atatgtcaat gattttaact
    22021 taagcaatcg agaaaaaata tatttaatac aaaggatatt taataatgtt gagtcgctat
    22081 ttaataaagt acctgtcagc attttagtta atgatatttt tctgaatgat ttttttatga
    22141 aaaacactga gatgattaac tggtactttc ctcggttact taagagttat gagggcgaaa
    22201 aaaattattt tgataattta aaatatgata taaatgatga gaaatctaat aaagaaattt
    22261 tgaagaatca accagataat gttatcaaag aaaaactgaa taatgaattc aaacttagat
    22321 ttagaatgat gcaaactatc cttaaatcga aggttaatgt atcgccatat attgaccaac
    22381 agcgtttaaa tacactaaat cctccggaaa atttacgtat ggcaatagaa aaatttggct
    22441 ggaagaaaaa aactatcact gcataaaata atttgatgcc gggatgtttt tgtatttccc
    22501 ggcatcttta tagagatatt aattatttaa tttactgagc gtcgcgacca tcaccgcttt
    22561 gatggtatgc atgcggtttt ccgcctgatc aaatacaatg ctggcggcag attcgaagac
    22621 ctcatcagtc acttccattc cgccatgtag gccaaactct tccgccattt ttttgccaag
    22681 cgtcgtttgg tcgtcatgaa acgcgggcag gcagtggagg aatttgacct ccgggttacc
    22741 ggtcaactgc atcatcttgc tgttcacctg ataatcacgc agtaatgcaa tgcgttcagc
    22801 ccatttttct tttgcttcac ccatcgacac ccatacatcg gtatagataa agtcagcacc
    22861 ttcaactcct ttagctacat cttccgtcag cgtaatattc ccgccatttt gttgtgccag
    22921 ggcgcggcat tccgtaacca gcgcagcttc tggccagcat gcctgtggcg cgaccagacg
    22981 caaatccaga ccggtaagcg ccgcagcttc gagcatcgaa ttgcccatgt tgttacgtgc
    23041 gtcacctgca tagaccagcg tcatttcgtt gaatgcttta ccgggtaaat gctcctgcat
    23101 ggtgaggaga tccgccagca actgcgtggg gtgaaactca ttggtcaggc cgttccatac
    23161 cggcacgcca gcgtattccg ccagtgtttc gacaatctcc tgaccatagc cgcgatactg
    23221 aataccgtca tacatgcgac caagcacgcg ggcagtgtct ttaatcgact ctttatgacc
    23281 aatctgacta ccgcttgggc caagataagt aacgcgagca ccctggtcat atgcggcaac
    23341 ttcgaaagag catcgggtac gagtcgagtc tttttcgaag atgagcgcga tgtttttacc
    23401 agtgagtttg gcttcttctt taccgctttt cttatcagct ttcagcttcg cggctaactg
    23461 cagcaggctg ttgagttcag ctggcgtgaa atcgagtaat ttcaggaaat gcttatgata
    23521 aaacccggac atagatccct cctgtggcta acgcctcaat gaattaaaat tcaatttata
    23581 tgtatgatta ttcatttgca agtctaaagc ataaatcttt gtcacaaagg tggaggcaat
    23641 gtcagtggtg tgtgacaata agagtatcgg caggacatta agaggaatga gccatggcaa
    23701 acccggaaca actggaagaa cagcgtgaag aaacacgttt gattattgaa gaattactgg
    23761 aagatggcag cgatccggac gcgctgtaca ccatcgaaca ccatctttcc gcagacgatc
    23821 tggaaactct ggaaaaagcg gcagttgaag cgtttaaact cggttacgaa gtgaccgatc
    23881 cagaagagct ggaagttgaa gatggtgata tcgtgatttg ctgcgacatc ctcagcgagt
    23941 gcgcgttgaa tgccgatctg atcgatgccc aggttgaaca actgatgacg ctggcggaga
    24001 aatttgacgt tgagtacgac ggttggggca cttactttga agatcctaac ggcgaagatg
    24061 gcgacgatga agattttgtc gatgaagacg atgacggggt tcgccactaa ttaattgatt
    24121 aaaatgcccg atagcacata ctagcgggca tttttttaga gttcgcgtag catccgcact
    24181 tcacaatcga catggcccgt gcagccaagc gcatagtcga tatgctcaaa gcccaaatgc
    24241 tcataaagcg caatggcttc ctttaaaaaa gcggtcgttt ccagatagca gcgtttgaag
    24301 cccatctctc gtgcctgctc catcgccatt aaggccagtt ttttcgctag ccctttaccg
    24361 cgaatagcgg gaagaaagta cattttctgc agttcgcaaa t
//
LOCUS       DBKEKI010000056        21863 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.056, whole genome shotgun sequence.
ACCESSION   DBKEKI010000056 DBKEKI010000000
VERSION     DBKEKI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.056"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(63..1193)
                     /gene="dnaJ"
                     /locus_tag="ACUQPX_002186"
     CDS             complement(63..1193)
                     /gene="dnaJ"
                     /locus_tag="ACUQPX_002186"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HHV9056892.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1282..3198)
                     /gene="dnaK"
                     /locus_tag="ACUQPX_002187"
     CDS             complement(1282..3198)
                     /gene="dnaK"
                     /locus_tag="ACUQPX_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HHV9056893.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3575..3979
                     /locus_tag="ACUQPX_002188"
     CDS             3575..3979
                     /locus_tag="ACUQPX_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HHV9056894.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4005..4718
                     /gene="msyB"
                     /locus_tag="ACUQPX_002189"
     CDS             4005..4718
                     /gene="msyB"
                     /locus_tag="ACUQPX_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HHV9056895.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLHGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            4867..5433
                     /gene="satP"
                     /locus_tag="ACUQPX_002190"
     CDS             4867..5433
                     /gene="satP"
                     /locus_tag="ACUQPX_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HHV9056896.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNITGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5468..6055)
                     /gene="mog"
                     /locus_tag="ACUQPX_002191"
     CDS             complement(5468..6055)
                     /gene="mog"
                     /locus_tag="ACUQPX_002191"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HHV9056897.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLASALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6170..7123)
                     /gene="tal"
                     /locus_tag="ACUQPX_002192"
     CDS             complement(6170..7123)
                     /gene="tal"
                     /locus_tag="ACUQPX_002192"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HHV9056898.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7402..8832
                     /locus_tag="ACUQPX_002193"
                     /pseudo
     CDS             7402..8832
                     /locus_tag="ACUQPX_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            8902..9678
                     /gene="yaaA"
                     /locus_tag="ACUQPX_002194"
     CDS             8902..9678
                     /gene="yaaA"
                     /locus_tag="ACUQPX_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HHV9056899.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(9831..10127)
                     /locus_tag="ACUQPX_002195"
     CDS             complement(9831..10127)
                     /locus_tag="ACUQPX_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HHV9056900.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10341..11627)
                     /gene="thrC"
                     /locus_tag="ACUQPX_002196"
     CDS             complement(10341..11627)
                     /gene="thrC"
                     /locus_tag="ACUQPX_002196"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HHV9056901.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11628..12560)
                     /gene="thrB"
                     /locus_tag="ACUQPX_002197"
     CDS             complement(11628..12560)
                     /gene="thrB"
                     /locus_tag="ACUQPX_002197"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HHV9056902.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12562..15024)
                     /gene="thrA"
                     /locus_tag="ACUQPX_002198"
     CDS             complement(12562..15024)
                     /gene="thrA"
                     /locus_tag="ACUQPX_002198"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HHV9056903.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15050..15169)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15105..15170)
                     /gene="thrL"
                     /locus_tag="ACUQPX_002199"
     CDS             complement(15105..15170)
                     /gene="thrL"
                     /locus_tag="ACUQPX_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HHV9056904.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15384..16070)
                     /locus_tag="ACUQPX_002200"
     CDS             complement(15384..16070)
                     /locus_tag="ACUQPX_002200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HHV9056905.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16470..16610)
                     /gene="yjjY"
                     /locus_tag="ACUQPX_002201"
     CDS             complement(16470..16610)
                     /gene="yjjY"
                     /locus_tag="ACUQPX_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HHV9056906.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            16706..17422
                     /gene="arcA"
                     /locus_tag="ACUQPX_002202"
     CDS             16706..17422
                     /gene="arcA"
                     /locus_tag="ACUQPX_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HHV9056907.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17481..18833)
                     /gene="creD"
                     /locus_tag="ACUQPX_002203"
     CDS             complement(17481..18833)
                     /gene="creD"
                     /locus_tag="ACUQPX_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HHV9056908.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(18891..20315)
                     /gene="creC"
                     /locus_tag="ACUQPX_002204"
     CDS             complement(18891..20315)
                     /gene="creC"
                     /locus_tag="ACUQPX_002204"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HHV9056909.1"
                     /translation="MRIGMRLLRGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPSEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20315..21004)
                     /gene="creB"
                     /locus_tag="ACUQPX_002205"
     CDS             complement(20315..21004)
                     /gene="creB"
                     /locus_tag="ACUQPX_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HHV9056910.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21017..21490)
                     /gene="creA"
                     /locus_tag="ACUQPX_002206"
     CDS             complement(21017..21490)
                     /gene="creA"
                     /locus_tag="ACUQPX_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HHV9056911.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            21701..>21863
                     /locus_tag="ACUQPX_002207"
     CDS             21701..>21863
                     /locus_tag="ACUQPX_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="AraC family; binds to the right arm of the
                     replication origin oriC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HHV9056912.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYI"
BASE COUNT         5212 a         5843 c         5311 g         5497 t
ORIGIN      
        1 cgcatatctt caacgcaccc tatttttacc caggcctgcc cacgggcagg cttttgggga
       61 ggttagcggg tcaggtcgtc aaaaaacttc ttcacaccat caaagaagct ctttgagcgc
      121 gggctgttgt gctcgccggt tgggccaccg aagctttctt gcagctcttg cagcagctgt
      181 ttctgctttt cgttcaggcc taccggtgtt tcgacgacaa cgcggcacag caaatcaccc
      241 tgtgcgccac cgcggacaga cttgacgcct ttaccgcgca tacggaatag cttaccggtc
      301 tgggtttcgc caggcacttt cagtttgacg cgaccatcaa gggtcggtac ttcgatttcg
      361 ccacccagcg ccgccatagc gaagttgatc gggacttcgc aatacaggtt gttgccttca
      421 cgctcgaaaa tcgggtgctg tttaacctga acctgaacgt acagatcgcc tgccggtgcg
      481 ccatgctcgc ccgcttcacc ttcgcccgca agacggatgc ggtctccagt gtccacccct
      541 gccgggattt taacggacag cgttttgctg cgctcaacac gaccatgacc atgacatttg
      601 ttgcacggat ctttgatcag cgtaccgcgg ccctgacagt gtggacaggt ctgctgtaca
      661 gcgaagaatc cctggcgcat ctgcacctga ccagaaccat gacaggtcgg acaggtctgc
      721 ggctgtgtac ctggttttgc accgctaccg tggcaaacgt cacactcttc cagagtcgga
      781 atgcggatct ctttggtcac gccacgtaca gcttcttcga gggtgagctc catgttatag
      841 cgtaaatcag caccgcgcgc cgcacgttga cgaccacgtc cgccgccaaa aatatcgccg
      901 aaaacgtcac caaaaatatc gctgaagtct gcgccgccgc caaaaccacc gccgcccatg
      961 ccaccttgct caaacgcagc atgaccatac tgatcgtatg ccgcacgttt ttgcgagtcg
     1021 gtcagaactt cataagcttc cttgatctct ttaaatttcg cctcggcctc tttgtcaccc
     1081 tggttacggt ccgggtggta tttcatggcc agccgtttgt aggcctttct gatttcacgc
     1141 tcttccgctg ttttggaaac gcctaaaatc tcgtaataat cttgcttagc catctttttt
     1201 aaattgcccc tagatgaatg cacgggcgga gaggaaattc cccttcgccc gtgtcagtat
     1261 aattacccgt ttatagggcg attatttttt gtctttgact tcttcaaatt cagcgtcgac
     1321 aacatcgtca tctttcgcgt tgtttgcaga agcatcagca ccggcagtct gctgctgggc
     1381 atgttgctgc tgggcgattt ccatcagttt ctgggaaacc tgtgccagtt cctgcatttt
     1441 cgcttcgata gcggctttgt cttcaccttt cagagcagtt tccagtgcag tcagcgcaga
     1501 ctcgatagca gttttgtcgt cagccggcag tttgtcgcct gcttcttcaa cctgcttacg
     1561 ggtgctgtgc agcagatggt cgccctggtt gcgagtctgt accagctctt caaacttacg
     1621 gtcagcttcg gcgttagctt ctgcgtcgcg taccattttc tggatttcat cttcgttcag
     1681 accagaagaa gccttgatgg tgatcttctg ctctttaccg ctgtttttat ctttcgcgga
     1741 aacgtgcagg ataccgtcag catcgatatc gaaggtaact tcgatctgcg gcatgccgcg
     1801 cggtgccggg ttgataccat ctaggttgaa ctgacccaga gatttgttat cagccgcacg
     1861 tttacgttca ccctgcagca catggatggt taccgcagac tggttgtctt cagcggtaga
     1921 gaacacctgg ctgtgcttgg tcgggatagt ggtgtttttc gcgatcagcg tcgtcatcac
     1981 accgcccatg gtttcgatac ccagagacag cggggtaacg tccagcagca gtacgtcttt
     2041 tacgtcacca gtcagaacac caccctgaac agcagcaccg attgctacag cttcgtccgg
     2101 gttaacgtct ttacgcggct ctttaccaaa gaactcagca actttcttct gaaccattgg
     2161 catacgagtc tgaccaccaa cgaggataac gtcgtcgata tcagatacgg acaggccagc
     2221 atcctgcagt gcaactttca gcggctcaat ggaacggttt accagatctt caaccaggct
     2281 ttccagtttc gcacgagtca ctttgatgtt catgtgtttc ggaccggtcg cgtctgcagt
     2341 gatgtatggc aggttaacgt cggtctgctg agcggaagac agttcgattt tcgctttttc
     2401 tgccgcttct ttcaggcgct gcattgccag cggatcgttg cgcaggtcaa tgccctgatc
     2461 tttcttgaat tcttcaacca gatagttgat cagacggctg tcgaagtctt caccacccag
     2521 gtgggtatca ccgttggttg ccagaacttc gaaggttttt tcgccgtcaa cttcgtcgat
     2581 ttcgataata gaaatatcga aagtaccacc ccccaggtca taaaccgcga tagtacggtt
     2641 gccagtgcct ttgtccagac cgtaagccag cgcagctgcg gtcggttcgt tgatgatacg
     2701 ttttacttcc agaccagcga tacggcctgc gtctttggtt gcctgacgct gagcatcgtt
     2761 aaagtatgcc ggtacggtga taacagcttc agttaccggt tcacccaggt aatcttcagc
     2821 ggttttcttc atttttttca gcacttcagc agaaatctgc ggcggtgcca ttttctggcc
     2881 tttaacttcg acccatgcgt cgccgttatc agcagcaata attttgaacg gcatgatgga
     2941 aacatcacgc tgtacttctt cgtcctggaa gcggcggcca atcaggcgtt taatcgcaaa
     3001 cagggtgttt tgcgggttcg tcactgcctg acgtttagcc ggctgaccaa ccagagtttc
     3061 accatcctgg gtataggcaa tgatagaagg cgtggtgcga tcgccttcgg cgttctccag
     3121 cacgcgagga gtggtgccat ccataatcgc tacacaagag ttggtagtac ccaggtcgat
     3181 accaattatt ttacccatct aaacgtctcc actatatatt cggtcatcat gtggttgtga
     3241 gtctgtaata ggggcgaaac gtctggtttc aactgcccaa tttcattttt ttgcagactc
     3301 actcactgcg gttgactact aaatggggtc gtaacccacg tcatcaaggg ggagatgcaa
     3361 aaattttttg tgcttttgcg cagaaatttc gcgatatggc aggacaagac gtaaaattga
     3421 cagcaatttt attcatataa agaatgaatc gcagccaagg cattcatcaa aaaattgtaa
     3481 taaaaagaaa agattacgtg cctgaatctt ctctttatca gcagtaaact agtgggtatt
     3541 catcccccta cctcttccca ctaagagaat ccttatgaaa tccgttttta cgatttccgc
     3601 cagcctggcg attagcctga tgctgtgctg cacggcgcag gcaaacgacc ataaactcct
     3661 cggcgtcatt gcaatgccgc gtaacgaaac caacgatctg gcgctgaaac ttcctgtttg
     3721 tcgcattgtg aaacgcatac aactctctgc cgaccatggc gatttacagt taagcggtgc
     3781 atcggtttat ttcaaagccg cccgtagcgc cagtcagagc ctgaatattc cttcagaaat
     3841 aaaagaaggg caaaccactg actggatcaa cattaacagc gataacgaca ataaacgctg
     3901 cgtctcaaaa atcacctttt ccggtcatac ggtgaactca tcggatatgg ccacgctgaa
     3961 aattatcggc gacgactaac gccagaatat agaagccaca aaaaatgaat gttaattacc
     4021 tgaatgattc agatctggat tttcttcagc attgtagtga ggagcagttg gcaaatttcg
     4081 cccgattgct cacccataat gaaaaaggca aaactcgcct ctccagcgta ctgatgcgca
     4141 acgaactgtt taaatcgatg gaagggcatc ccgagcaaca tcgccgcaac tggcagctga
     4201 ttgccggaga attacagcat tttggtggcg atagtatcgc caacaaactg cgcggacacg
     4261 gtaaattgta tcgggccatt ttgctcgatg tctcaaagcg attgaagctg aaagccgaca
     4321 aagagatgtc tacgtttgaa attgagcaac agttactgga acaatttctg cgtaatacct
     4381 ggaagaaaat ggacgaggaa cataagcagg agtttctgca cgcggtcgat gccagggtga
     4441 atgagctgga agagctgctg ccgctgctga tgaaagacaa attattggca aaaggtgtgt
     4501 cgcatttgct ttccagccaa ctgacccgca ttttacgcac ccacgcagca atgagcgtac
     4561 ttgggcatgg tttgctgcac ggcgcggggc tgggaggccc tgtgggcgca gcattaaatg
     4621 gggttaaagc ggtcagcggc agcgcctatc gcgtgacgat tccagccgta ctgcaaatcg
     4681 cctgcctgcg ccggatggtt agcgccactc aggtctgata ccggaaaccg gggaaaattt
     4741 tcattttccc ctcaaaagat cgtagacact gccccactgg ctgattatta tgccgcgccc
     4801 tgaaaacact acagttattc agggaaatta tttcaccatt cattcgatga tgatttttga
     4861 ggaattatgg gcaacactaa gttggctaat ccggcaccgc tgggcctgat gggcttcggc
     4921 atgaccacca ttctgcttaa cctgcacaac gtgggttatt tcgctctgga cggtattatt
     4981 cttgccatgg gcattttcta cggcggcatc gcgcaaattt ttgctggtct gctggagtac
     5041 aaaaaaggca acactttcgg tttaaccgca ttcacctctt acggttcttt ctggctgacg
     5101 ctggttgcga ttctgctgat gccgaaactg ggtctgaccg atgcgccaaa tgcacagttc
     5161 cttggtgtct acctgggtct gtggggcgta tttacgctgt ttatgttctt cggcactctg
     5221 aaaggcgcac gcgtcctgca attcgttttc tttagcctga ccgtgctgtt tgccctgctg
     5281 gcgatcggta acattaccgg taatgccgcg atcatccact ttgccggctg gattggtctg
     5341 atctgcggtg ccagcgcaat ctatctggcg atgggtgaag tgctgaacga gcagtttggt
     5401 cgcaccgttc tgccgattgg tgaatcccac taattgtctt gagatccccc cgctcggggg
     5461 gattttttta ttcgctaacg tcgcgtcttg cactcttcgg tctgaatgca gcaaccactt
     5521 ctggtgccgt ttcaacgtat ggcccttcca gcaactgaat gcagtacggt acgctggcaa
     5581 aaataccatg caccacaacg ttaccctcag cgtccttcac accttccagc gtctctttaa
     5641 tagacttcgg ctgaccgggt aagttaagga tcagcgcctg tttgcgaatc acgcccacct
     5701 gacgcgaaag gatcgcagtt ggtacaaaat gcaggctgat ctggcgcatc tgttcaccaa
     5761 agccaggcat ctcgcggtcc gctactgcca gcgtcgcatc gggcgttacg tcacgacgcg
     5821 ccgggccagt tccgcccgtg gtgagcacca gatggcaact catttcatcc accagctcac
     5881 acaacgtttg ctcgatgatc gcctgctcat cggggattaa gcgggtttcc agttcaaacg
     5941 gcgtggttag cgccgatgcc agccattctt ccagcgctgg aatgccttta tcctgataaa
     6001 cgccgctgga tgcgcgatca gagatggaaa ctaagccaat acgtaaagta ttcatcaaga
     6061 tttttccgtt taaacagtct cgttaaacag aatgatacac tgcgaaggga gtgacagaca
     6121 ggcttcagaa gaggtagcgt gaccgacttc ccggtcacgc taagaatgat tacagcagat
     6181 cgccgatcat tttttccagt ttttcctggt caatagcaaa cttacggata ccttccgcca
     6241 gtttatctac tgccattgga tcctggttgt gctgccacag gaactcggac tcagtgatac
     6301 gcgccggacg cgctttcact tcgccggtgt aagacagttt acgttcgata gccccttcgc
     6361 tctccgccag ctctttcagc agtgccggtg cgatggtcag acggtcacag cctgccagtt
     6421 ccagaatttc gccgatgtta cggaagcttg cgcccataac cacggtttca taaccgtgct
     6481 ctttgtagta ctggtagatt tcagatacag aaaccacgcc cggatcttct gccggagcgt
     6541 actctttctt atcggtattc gctttgtacc agtcaagaat acggccaaca aacggcgaga
     6601 tcaggaacac gcccgcctcc gcacaagcac gagcctgagc gaaggagaac agcagggtca
     6661 ggttacagtt gataccttct ttttccagct gttctgcagc acggataccc tgccaggtag
     6721 aagccagttt gatcagaata cgatcgttgc taatacctgc atcgttgtag agtttgatca
     6781 ggcgttttgc tttcgcaatt gacgcttcgg tgtcatagga aagacgcgca tcaacttcag
     6841 ttgagatacg gcccggaacc agtttcagga tttccagacc aatatttact gccagtttgt
     6901 cggtcgcgtc cacgatctgc tgcgcgcgat cgttgctctg ctgtttcgcc caggcgacag
     6961 catcatcaat caacttacgg tattccggaa tctgcgctgc gttaagaatg agagaagggt
     7021 tggttgtggc atcctgcggt tgatacagct tcattgccgc gatgtcccca gtgtcggcca
     7081 ctacggtggt gtactgacga agggaggtca atttgtccgt catgatagta tttctcttta
     7141 aacagcttgt tagggggatg taaccggtct gccctgatga tatcacgacg caacgtcagc
     7201 gcaaccgccg acaagacggt tatcgccagg cgagactgtt tcggatttct gacactgcgt
     7261 tgatatcgcc cgccattttt atacaaaacc tcatgtatgc tacgcagaag ttatcaagta
     7321 cctcgtagcg tatatacttc ttaaacaatt ggtaacgttt acacaggaaa gtcatcgcga
     7381 ccggcaataa gagggatatg catgccagat tttttctcct ttattaacag cgtcctttgg
     7441 ggatcggtaa tgatttacct gctcttcggc gcaggttgtt ggttcacttt tcgcaccgga
     7501 tttgtgcagt ttcgctacat ccgccagttt ggcaaaagtc ttaaaaatag cattcatcca
     7561 tagccaggcg gtttaacctc atttcagtca ttgtgtacca gtcttgcggc gcgcgtgggt
     7621 agcggcaacc tggccggcgt tgcgctggca attaccgccg gtggaccagg tgccgtcttt
     7681 tggatgtggg ttgctgcgtt tatcggcatg gcgacctcgt ttgccgaatg ttcccttgca
     7741 caactttata aagaacgtga cgtcaatggg cagtttcgtg gcggaccggc atggtatatg
     7801 gcgcgcgggc tggggatgcg ctggatgggc gttctgttcg ccgtcttttt gctcatcgcc
     7861 tacggcataa ttttcagcgg agttcaggcg aacgccgttg cccgagccct gagtttttct
     7921 tttgattttc ctccgctggt gacaggcatt attctcgctg tctttgctct gctggcaatc
     7981 actcgcggtc ttcatggcgt cgcccggctc atgcaggggt ttgtcccgtt gatggcgata
     8041 atctgggtac tgaccagcct ggtgatttgc gtaataaata tcgggcaact tccccacgtc
     8101 atttggtcta tttttgagag tgcttttggc tggcaggaag cggcaggcgg cgcggcggga
     8161 tataccttaa gccaggcgat tactaacggt tttcagcgca gtatgttttc caatgaggcg
     8221 ggaatgggat cgacgccaaa cgcggcagcg gcagctgcgt cctggcctcc gcatccggca
     8281 gcacaaggga ttgtccagat gattggcatt tttatcgaca ccctggttat ctgtacggca
     8341 agcgccatgc tgatattact ggcgggtaac ggcacaacct acatgccgct ggaaggtatt
     8401 cagcttatcc agaaggcgat gcgggtgcta atgggtttct ggggtgctga atttgttacc
     8461 ctcgtggtta ttctgtttgc cttcagctcc atcgttgcca actacattta tgccgaaaac
     8521 aatctcttct ttttacgcct gaacaaccct aaagcgatct ggtgtttgcg gatctgcacc
     8581 ttcgcaacgg tcatcggcgg caccttgcta agccttccgc tgatgtggca actggcagat
     8641 atcataatgg cctgcatggc tattaccaat ttgaccgcca ttttactgct ctcgcctgtg
     8701 gttcatacca ttgccagtga ttatctacgc cagcgtaaac tcggcgtgcg cccggtgttt
     8761 gatccgttgc gttatccgga tatcggtcgc cagctttctc cggacgcgtg ggatgatgtt
     8821 tcgcaggagt aatcacaact atcgatcaac tcattctctt tttttgctaa agtcggcata
     8881 aatttcctgc aaggactgga tatgctgatt cttatttcac ctgcgaaaac gcttgattac
     8941 caaagcccgt tgaccaccac gcgctatacg ctgccggagc tgttagacaa ttcccagcag
     9001 ttgatccatg aagcgcggaa actgacgcct ccgcagatta gcacgctgat gcgcatcagc
     9061 gacaaactgg cgggtatcaa cgccgctcgc tttcatgact ggcagccaga tttcacgccg
     9121 gcgaatgccc gccaggcgat tctggcgttt aaaggtgatg tctacaccgg cttgcaggcc
     9181 gaaaccttca gcgaagacga tttcgatttt gcccaacagc atttgcgaat gctttccggc
     9241 ttgtatggcg tactgcgccc gctcgattta atgcagcctt atcgtctgga aatggggatc
     9301 cgtcttgaga atgcccgagg gaaagatctg tatcaattct ggggagatat catcaccaac
     9361 aagctgaacg aggcgctcgc agcacaaggc gataatgtgg tgattaatct ggcgtcagat
     9421 gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg agattatcaa gccggtgttc
     9481 ctcgatgaga agaacggcaa atttaagatc atcagcttct acgctaagaa agcacgcggt
     9541 ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac cagagcaact gactggtttt
     9601 aatagcgaag gttacttctt tgatgaagat tcctccagca atggcgaact ggtgtttaaa
     9661 cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc ggcgtgaacg ccttatccgg
     9721 cctacaaaag catgcagatt caatatattg cagaattaac gtaggcctga taagacgcgc
     9781 cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat ttagcggtga
     9841 tgtttgcctg gaccatgacc gccgtgatga tcatgaggag ctttcttatg gtggcgcggc
     9901 ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg ttgtttccac
     9961 cagccgtggt cgcgccagtg acctccatcc cagtaatagc cacgattatc acgatcgcct
    10021 atctgtaatt ttactgacgg gactaacgta atttccgcag cctgtgctgc catgggagcg
    10081 accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg ttactccttt
    10141 tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacga cggttgttat
    10201 tctccgcaat cctaatctct aatttaccgc atttattgag aatttctcct ttttccctgt
    10261 cgtttttctc cataatttct tcataaaaaa gccgggctgc ataaaagcaa acccggcctg
    10321 attgagataa tgaatagatt ttactgatga ttcatcatca atttacgcaa cgcagcaaaa
    10381 tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag ctcttttggc
    10441 agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc cggatgcgcg
    10501 gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc acgatacgct
    10561 acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat tgtctgttgc
    10621 gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat tttgcggcgg
    10681 aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc gttggataac
    10741 gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg
    10801 ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgaatgactt cgccagcaga
    10861 cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg gttgcgcgcc
    10921 tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag caaacggctg
    10981 atgttaatcg agttagccga gtttaacccc agcgccactt tcagttcttc atcatcaaac
    11041 gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac agtttcgata
    11101 ttgccgccca acgtacagaa cagtttttct tgcagtggac tgattttgcc tcgtggatag
    11161 aggataacca ctttcacatt cggcaaaccg tagaaagcat gagccactgc agctccggta
    11221 tcaccggagg tcgcggtcag aatggtcact ggcttatcat ccgcaatatg ggtcagcatt
    11281 tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg gaacaattcc
    11341 agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa cgccgcgcgc
    11401 acgcgctctt ccaggatttc ctgtgggatt tcatcaccaa taaacgccga gaggatcttc
    11461 gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag gctgaattcc
    11521 ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca acccctgggt tacggcttgc
    11581 gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta gttttccagt
    11641 actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg attttgcagg
    11701 tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg
    11761 aacaaggtcg ggccggagcc ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc
    11821 tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttcagc gataacatct
    11881 ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat gaagccagcc
    11941 agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa aatagcccgg
    12001 gcttctgccg tcgagacttt aattcccgga tacgccagca cccacagcca ctcatcaaac
    12061 cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat accaccaaga
    12121 aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga tacgtccttc cagctcgccc
    12181 atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc agtgttcatt catcgccatc
    12241 agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgatcgg catattcttt
    12301 tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg ctcccagcac
    12361 tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg tccgaggttg
    12421 ttgagactga atgtctctgc cgcctcaacc gtgactacat ctccgagcaa tgcaccatca
    12481 acgggtgcca ccgccgcccc gagcacatca aacccgacgc tcatattggc actggaagcc
    12541 ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac gtagcagatc
    12601 ggcaaagaca ccggcagcgg taacgtcatt gcccgcacca tatccgcgca gcaccaacgg
    12661 cagcggctga taatagtggc tataaaaggc cagggcgttt tcgccatttt tcactttgaa
    12721 cagcggatca ttcccatcca cttcggcaat cttcacgcgg cagacaccat cttcatcaat
    12781 attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa
    12841 gagatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct cggcgttaaa
    12901 ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac gtcccgtttc
    12961 acgagcgaga atcaatagtt tacgcgccac atccatacca gaaagatcat ctcgcgggtc
    13021 cggttcggta taacccattt cccgcgccag cgtggtcgcc tcggagaaac tcatgccttc
    13081 gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga acttcaccaa
    13141 ttcatcacct gcattgagca gattttgcag gttctcaata accggtaatc cagccccaac
    13201 gttggtgtca tagaggaatt tacgccgcga tttttccgcc gcataacgca actgatgata
    13261 gtaatccatc gacgaggtgt tggccttttt gttcggcgtg acaacgtgga aaccttcgcg
    13321 caggaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa caatgaccgg
    13381 gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa acggctcttt
    13441 ggcttgtgca agttcttcct gccagttttc cagattaagg ccatgcacac tagtgagcag
    13501 agccttcgag ttggcaacgc cgcagacacg taagtcgata tgtttattct tcagccagct
    13561 ttgctgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc caatcacaaa
    13621 cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca cgccagtggt
    13681 cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct gagcaatggc
    13741 gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa
    13801 ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca ccgccagcgg
    13861 ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc gttcagctcg
    13921 cacacagtcg ctttgtggaa cgcagaaact gatactgtat tcggaagatg attgcgtaat
    13981 cagcaccacg gaaatacggg cgcgtgacat tgcagcaaag acgcgcgccg ccatgccaac
    14041 catccctttc atccccgggc cggaaacgct gaacattgcc atgttattca gattggaaat
    14101 gcccttgacc ggtaattcgt cttcatcacg gctggcacca atgagcgtac ctggtgcttg
    14161 aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg gggtaatggt
    14221 gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct ggtaggacat
    14281 cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataga ccccgtcaac
    14341 gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg ccgcggagta
    14401 gtcggaaccg ttacgtccaa gcaccaccag ttcgcctttt tcattaccgg cggtgaaacc
    14461 tgccatcaac accatgtgat cagccggaat gcggcttgcc gcaatacggc gggtggactc
    14521 agcaatatcg acggtagatt cgaggtaatg ccccactgcc agcagttttt cgaccggatc
    14581 gataacggta acgttgtgac cacgcgcttc taacacgccg gccataatgg cgatcgacat
    14641 tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc ccaacaaact
    14701 aatgccatgc aggacatgtt ttatttgggc aaattcctga tcgacgaaag ttttcaattg
    14761 cgccagcggg aaccccggct gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg
    14821 ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt caatcatcgc
    14881 caccaggtgg ttggtgattt tggcgggggc agagaggacg gtggccacct gcccctgcct
    14941 ggcattgctt tccagaatat cggcaacacg cagaaaacgt tctgcatttg ccactgatgt
    15001 accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg aaaaaaaagc
    15061 ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta
    15121 cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg
    15181 tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt atttagtgac
    15241 ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag ctgctatcag
    15301 acactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa tgaaaagctg
    15361 aaaaatactt actaaggcgt tttttatttg gtgatatttt tttcaatatc atgcagcaaa
    15421 cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgctg taaccagtcg
    15481 accagtttta tgtcatctgc cactgccaga gtcgtcagca atgccatgac tcgttcgcgt
    15541 aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg ttgtattaat
    15601 gttgctaatt gatagcaata gaccatcacc gcctgcccca gattgagcga aggataatcc
    15661 gccaccatcg gcacaccagt aagcacgtca gccaacgcta actcttcgtt agtcagcccg
    15721 gaatcttcgc gaccaaacac cagcgcggca tggctcatcc atgaagattt ttcctctaac
    15781 agcggcacca gttctactgg cgtggcgtag taatgatatt tcgcccgact acgcgcagtg
    15841 gtggcgacag tgaaatcgac atcgtgtaac gattcagcca gtgtcgggaa aactttaata
    15901 ttatcaataa tatcaccaga tccatgtgcg acccagcggg tggctggctc caggtgtgcc
    15961 tgactatcga caatccgcag ttcgctaaaa cccatcgttt tcattgcccg cgccgctgcc
    16021 ccaatatttt ctgctctggc gggtgcgacc aggataatcg ttatacgcat attgccactc
    16081 ttcttgacca aataaccgcg aacagggtga tcactgtcaa cttattacgc ggtgcgaatt
    16141 tacaaattct taacgtaagc cgcagaaaaa gccctttact tagcttaaaa aaggctaaac
    16201 tattttctga ctgcactaac ggtttaattg ttaaaaaatg ctacatatcc ttctgtttac
    16261 ttaggataat tttataaaaa ataaatctcg acaattggat tcaccacgtt tattagttgt
    16321 atgatgcaac tagttggatt attaaaatta tgtgacgaaa gctagcattt agatacgatg
    16381 atttcatcaa actgttaacg tgctacaatt gaacttgata tatgtcaacg aagcgtagtt
    16441 ttattgggtg tccggcccct cttagcctgt tatgttgctg ttaaaatggt taggatgaca
    16501 gccgtttttg acactgtcgg gtcctgaggg aaagtaccca cgaccaagct aatgatgttg
    16561 ttgacgttga tggaaagtgc atcaagaacg caattacgta ctttagtcat gttacgccga
    16621 tcatgttaat ttgcagcatg catcaggcag gtcagggact tttgtacttc ctgtttcgat
    16681 ttagttggca atttaggtag caaacatgca gaccccgcac attcttatcg ttgaagacga
    16741 gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg atgttttcga
    16801 agcgacagat ggcgcggaaa tgcatcagat cctctctgaa tatgacatca acctggtgat
    16861 catggatatc aatctgccgg gtaagaacgg tcttctgtta gcgcgtgaac tgcgcgagca
    16921 ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata aaattctcgg
    16981 cctcgaaatc ggtgcagatg actacatcac caaaccgttc aacccgcgtg aactgacgat
    17041 tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt actgtcagcg aagaacgtcg
    17101 tagcgttgaa agctacaagt tcaatggttg ggaactggac atcaacagcc gttcgttgat
    17161 cggccctgat ggcgagcagt acaagctgcc gcgcagcgag ttccgcgcca tgcttcactt
    17221 ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg ctgaagaaaa tgaccggccg
    17281 tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc cgccgtattc gtaaacattt
    17341 cgaatctacg ccggatacgc cggaaatcat cgccaccatt cacggtgaag gttatcgctt
    17401 ctgcggtgat ctggaagatt aatcggcttt accaccgtca aaaaaacggc gctttttagc
    17461 gccgttttta tttttcaacc ttatttccag atacgtaatt catcgtccgt tgtaacttct
    17521 ttactggctt tcattttcgg cagtgaaaac gcataccagt cgatattacg agtcacaaac
    17581 atcatgccgg ccagcgccac caccagcaca ctggttccca acaacagcgc gctatcggca
    17641 gagttgagca gtccccacat cactccatcc agcaacaaca gcgcgagggt aaacaacatg
    17701 ctattgcgcc agcctttcaa taccgcctgc aaataaatac cgttcattaa cgccccaatc
    17761 agactggcga ttatccatgc cacggtaaaa ccgatatgtt cagaaagcgc cagcaagagc
    17821 aaataaaaca tcaccaatga aagccccacc agcaaatatt gcattgggtg taaacgttgc
    17881 gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca gtgcaatcag cagaatggcg
    17941 tacttagtcg cccggtccgt taattggtat tgatcggctg gcgtcgtcac tgcgacgcta
    18001 aacgcgggga agttttccca gccggtatca ttgcctgaag caaaacgctc accgagatta
    18061 ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg actcgctagc ttcccgtttg
    18121 gctggtagaa aatcacctaa aaaactggga tgcggccagt tgctggttaa ggtcatttcg
    18181 ctattacgcc cggcaggcac cacagaaaga tcgcctgtac cgcttaaatt cagtgccata
    18241 ttcagctgca ggttctgctt ccgccagtcc ccttcgggta aagggatatg cacgccctgc
    18301 ccgccttgct ctaacccggt accgggttca atggtcagtg ccgttccgtt aacttcaggc
    18361 gctttcacca caccaatacc acgcgcatcc ccgacgctaa tcacaataaa gggcttgcct
    18421 aagatgatat ttggcgcgtt gagctcgcta agacgcgaaa catcgaaatc ggcttttagc
    18481 gttaaatcac tgtgccagac ctgaccggta taaatcccta tcttgcgttc ttccacgttc
    18541 tgattgccat caaccatcaa tgactcaggt aaccaaaaat ggataaaact tcgtttccgc
    18601 tccacggttt tatcctcttc ctgcaccgta taaagctcgg tcacaggaat agcgataagc
    18661 ggcccaacga gtttttgcgg cccgctggta ctttggcgaa tcgcatcttc cacatcgcta
    18721 cggtaatcgg cacgttcgac aatcacttgc cgaatcagca ttatcggaat caacaacagc
    18781 aatactgcac caaacaaggt ggtcattttc cagaacaggg gggatttcaa catagtcttc
    18841 tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa gaatttgaag ctatgtgaag
    18901 tgacggtgaa gtcgtagcga ggccagcacg ccaccttcct gcacgttgcg cagcgtgact
    18961 tcgccgttaa acaaacgggc gacctcactg acgaacgcca accccagacc gctgcttttt
    19021 tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag cgcgtagtca
    19081 ggaataccac taccggtatc cagcacctta agcgtgacgt gttcctgatc cacttcggcg
    19141 cttagcgtta tgcaaccgct ctcgggagta aaatcgatgg cgttatccag taaattcccc
    19201 agcgcctgat ccagtaacgc cggttcagca gcaacattaa cctcggaggg cataacatgc
    19261 aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg gcgaaataat
    19321 gccgccacat caacagcagt cagaacgact tcctgacgat tctccagtct tgcctggcgt
    19381 agtaacgttt ccaccagcgc ctgcattcgc gcattttgtg tcagaatgtt gtcagtaaaa
    19441 cgagccacta cttcaggcgg cggaccttcg cgtaaaattt ccgccgcgcc acgaatcgcc
    19501 gccagtgggc tttttagctc atgggttaac gcataaacat actgctcaat atagtttttc
    19561 ccttccagct tcacgcgcat actttccagc gcctgcgcga gtttacgcag ctcgctacta
    19621 ccgagatcgg gaagaggaac gggcttattg tcagtgacgg aatcagcata gcgagtgagc
    19681 cgagcaatgg agcggttgat ccaccaaacc atgcctgcgc caatcaccag tgcaatcccc
    19741 aacaaaatgg cgctggccca taaaattcgc cgctcgctgc gtttaatgac cggagccatc
    19801 gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc gcgagccgtc cataatcggc
    19861 gcggcaacat acatcaccga actttcggga tcggcaggat tttgcaacgt gctgcgcgca
    19921 ccatactgac cacgcaacgt tagccagacg tcattccagc gcgaataatc ctgtccaacg
    19981 gctttatttg ccgaatcgaa caatactttg ccatgcgcat cggtcatata gacatgatat
    20041 tcattgcgca ctttgttaat gccaccaata ttggcgcgaa acgggcgatg ttgtagctga
    20101 ttaaacgcct gcgccagttg cccatgcgtt gggtccccag agagcaaatc gggacgcgcc
    20161 agctccgcaa gtaacgttgc cgtgtcgatc aacgtcccct ccgttgctct tcgcacgccc
    20221 ggtttaactt ctttgacaaa aatagccagt acgaaccagg ccgccaccgc caccagtaaa
    20281 aaatagcccc gcagcaaccg catgccgata cgcattacaa gcccctcagg ctatatccca
    20341 tgccgcgatg agtattaatc ggtgaaagat cggggttgat ggcgcgcagc ttggcacgca
    20401 gcgttttaat gtggatatcg acggtgcgat cgtaggtgtc ctgcgcatct tcccacacgc
    20461 tatccattag ttgctggcgg gaccagacgc ggcctggcga cttgagtaac gtcttcagca
    20521 ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc tgcgccgcgg
    20581 gttcattcaa ttcaaaatga ccaatacgga tgacgggaga cggcgtcgag aacttcttca
    20641 cccgacgcag taaggtgcgc accctggcgc acacttcgcg gggtgaaaac ggtttagcca
    20701 catagtcgtc agcgccaatt tccagcccaa gcaggcgatc gacctcttca cttcgggccg
    20761 tcaggaacag tacaggtaac gccggatgga gcgccagtaa ctggcggcac aattcaaagc
    20821 cactgatatc aggcaaacca acgtcgagaa tcatgacgtc gggaacctgc tggcgagctt
    20881 tatccagcac cggcaaacct cgctcaaaga cctcgacggc aaaaccttcc tgctgcaaca
    20941 tgtagaccag cgtgtcggct atcccttgct catcttccac taaccagacc gtttcccgtt
    21001 gcataaatcg cctctgttat tgccgccacg gcatgacagg aaccgcgcta atcgcgtttt
    21061 tcggcgaacc ttctacaact ttgtcggagt aagccagata agcgagcgcg ttgcgtttgg
    21121 cgtcataaaa gcgcacgacc tgtaacgact taaagaccag ggacgtgcgt tttttgaata
    21181 ctacctcgcc ctgagctttg ccgtttttaa tacgatccga cagttcaatc ggcccgactt
    21241 gctgacaaga aatggccgca tcggaggtat cttctgccag gcccaatccc cctttaatac
    21301 caccggtttt cgcccggctc acataacagg tgacattttt cacatcggga tcgtcaaagg
    21361 cttccacaac aattttgtga tccgggccga tcattttaaa tacggtgtcg accgaaccaa
    21421 tctcttctgc atgagccaat ggccccagca ttatcaggct taaagacagg atcaaatgct
    21481 tgtatttcat attgttacca tttttatcta ttacacgagc aattagttcg tcacggtaaa
    21541 taacatattg attaagaaca aaatctcaat tcttttattt ccgtcaagcc ctaaaacata
    21601 ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta tccaattacc
    21661 tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt atggatcagg ccggcattat
    21721 tcgcgacctt ttaatctggc tggaaggtca tctggatcag cccctgtcgc tcgacaatgt
    21781 agcggcgaaa gcaggttatt ccaagtggca cttacagaga atgtttaaag atgtcactgg
    21841 ccatgctatt ggcgcgtata ttc
//
LOCUS       DBKEKI010000057        21731 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.057, whole genome shotgun sequence.
ACCESSION   DBKEKI010000057 DBKEKI010000000
VERSION     DBKEKI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.057"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..784)
                     /locus_tag="ACUQPX_002208"
     CDS             complement(<1..784)
                     /locus_tag="ACUQPX_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HHV9056913.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMS"
     gene            1132..2004
                     /gene="rluF"
                     /locus_tag="ACUQPX_002209"
     CDS             1132..2004
                     /gene="rluF"
                     /locus_tag="ACUQPX_002209"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HHV9056914.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2137..2409)
                     /locus_tag="ACUQPX_002210"
     CDS             complement(2137..2409)
                     /locus_tag="ACUQPX_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HHV9056915.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLNNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(2662..4011)
                     /gene="lysC"
                     /locus_tag="ACUQPX_002211"
     CDS             complement(2662..4011)
                     /gene="lysC"
                     /locus_tag="ACUQPX_002211"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HHV9056916.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(4103..4299)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            4536..6185
                     /gene="pgi"
                     /locus_tag="ACUQPX_002212"
     CDS             4536..6185
                     /gene="pgi"
                     /locus_tag="ACUQPX_002212"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HHV9056917.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQGLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            6647..6895
                     /gene="yjbE"
                     /locus_tag="ACUQPX_002213"
     CDS             6647..6895
                     /gene="yjbE"
                     /locus_tag="ACUQPX_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004387525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HHV9056918.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTTTSTQ"
     gene            7009..7647
                     /locus_tag="ACUQPX_002214"
     CDS             7009..7647
                     /locus_tag="ACUQPX_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HHV9056919.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            7644..8381
                     /locus_tag="ACUQPX_002215"
     CDS             7644..8381
                     /locus_tag="ACUQPX_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HHV9056920.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRVPQ"
     gene            8381..10477
                     /locus_tag="ACUQPX_002216"
     CDS             8381..10477
                     /locus_tag="ACUQPX_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HHV9056921.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(10524..10802)
                     /locus_tag="ACUQPX_002217"
     CDS             complement(10524..10802)
                     /locus_tag="ACUQPX_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056922.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            10803..10946
                     /locus_tag="ACUQPX_002218"
     CDS             10803..10946
                     /locus_tag="ACUQPX_002218"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056923.1"
                     /translation="MFQRIIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            11072..11482
                     /gene="psiE"
                     /locus_tag="ACUQPX_002219"
     CDS             11072..11482
                     /gene="psiE"
                     /locus_tag="ACUQPX_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HHV9056924.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(11526..13001)
                     /gene="xylE"
                     /locus_tag="ACUQPX_002220"
     CDS             complement(11526..13001)
                     /gene="xylE"
                     /locus_tag="ACUQPX_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HHV9056925.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDSTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(13373..14263)
                     /gene="malG"
                     /locus_tag="ACUQPX_002221"
     CDS             complement(13373..14263)
                     /gene="malG"
                     /locus_tag="ACUQPX_002221"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HHV9056926.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(14278..15822)
                     /gene="malF"
                     /locus_tag="ACUQPX_002222"
     CDS             complement(14278..15822)
                     /gene="malF"
                     /locus_tag="ACUQPX_002222"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HHV9056927.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITTILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(15976..17166)
                     /gene="malE"
                     /locus_tag="ACUQPX_002223"
     CDS             complement(15976..17166)
                     /gene="malE"
                     /locus_tag="ACUQPX_002223"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HHV9056928.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            17531..18646
                     /gene="malK"
                     /locus_tag="ACUQPX_002224"
     CDS             17531..18646
                     /gene="malK"
                     /locus_tag="ACUQPX_002224"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HHV9056929.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            18718..20058
                     /gene="lamB"
                     /locus_tag="ACUQPX_002225"
     CDS             18718..20058
                     /gene="lamB"
                     /locus_tag="ACUQPX_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HHV9056930.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            20210..21130
                     /gene="malM"
                     /locus_tag="ACUQPX_002226"
     CDS             20210..21130
                     /gene="malM"
                     /locus_tag="ACUQPX_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HHV9056931.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSIFISSVKGKG"
     gene            21359..>21731
                     /locus_tag="ACUQPX_002227"
     CDS             21359..>21731
                     /locus_tag="ACUQPX_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HHV9056932.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVTGLNRDN
                     FIYISDIFLNIKQRNEKNHAINMLREVSISNDTISVKFYRNEKIECACNFLMDKDAQG
                     YIDLSDFDLTNCHFKGDVISKV"
BASE COUNT         5455 a         5542 c         5584 g         5150 t
ORIGIN      
        1 cgctcatatt cgtttcaacc attgcgccgt gaatatcaaa aaagcgcgag caaatatcgc
       61 cagcaacctg gcgggcattc aggtcgtcac tctcttcacc accataaaac gcatgccagt
      121 tagcgccgtc gcgaatggcc ggtgagccaa ttcccaccag ggcgacatcc agattatccc
      181 agtaggcaga gatggtttta aagtgctgag actgcatgat cccattacga attaatgggt
      241 tatccagcag agccggaaaa tccgcgagat gcgattcccc tttcagcttc gctgccgcgc
      301 tgtaggttaa tgtgtttaca tgatagcggc tttcgagttt accggacggt ccgccaataa
      361 ttggtacgca gattaactgc cgcgattgcc ccgcctgcgg caagttttca accagtgcgc
      421 tcaccgcgcg accccaggaa aaaccgacaa tatcgccagg ctccagcaag cgatccagca
      481 gttgcgcgcc gtgtaacccc atcatcgcca gttgagtctc ttcatcctca tcatttcccg
      541 acaccaccac aacgtctttc aggccaaact tttgcttcag ttgctgctcc agccagagat
      601 tttcgttgta gtcatagttg atggcgatgg tgaccattcc ctgatcgcgg cctcgtttaa
      661 gcaagcggct gatggtggtg cggtaaatac ccagttcgcg cgcgatttgc gcctgcgtca
      721 tatcctgttc gtaatagagt tgggcaatct tcacaatcaa acggatatcg tcactgtttt
      781 ccattgccgc ctcctggttc tgcacatttg tgcaaatacg ctttctcacc gcttgatctg
      841 atataacccc atcatgaggg cctttcaaag cgatttttca ctctttgcat ggggagaaat
      901 agcgctttct cttgcacatt tgtttctctg cgtagtagtc atcagcacag gtgtgcagcg
      961 attcgtaata tgttgattat ccttattttg ccaggcaaat ttgcatcgta cttccggaga
     1021 cacttttgtg ataaaaggcg tggcagatca catttaattt cgacgttgcc gcataaccct
     1081 gtttttcccc gctaagaggt acggtatatt ccgcgtttta caggagaatt tatgctgccc
     1141 gactcatcag tccgtttaaa taaatacatc agcgaaagcg gaatttgctc acgccgcgaa
     1201 gcggatcgct atatcgagca aggcaatgtg ttccttaatg gcaagcgagc caccattggc
     1261 gatcaggtga aacccggcga cattgtgaaa gtaaacggtc agttgattga gcctcgggaa
     1321 gccgaagatt tggtacttat cgccctgaac aagcccgtcg gtattgtcag caccaccgaa
     1381 gatggcgagc gcgataacat tgtcgatttc gttaaccaca gcaaacgcgt gttcccgatt
     1441 ggccgcctgg ataaagactc ccaggggctg attttcctca ccaatcacgg cgatctggtg
     1501 aataagatcc tccgtgcggg taacgatcat gagaaagagt atctggtgac ggtcgataaa
     1561 ccgattaccg atgaatttat tcgtggtatg ggggcggggg tgccgatcct cgggacggtg
     1621 acgaaaaagt gcaaagtcaa aaaagaagcg ccgtttgtct tccgcattac cctagtgcag
     1681 gggctgaacc gtcagatccg gcgcatgtgc gagcatttcg gctatgaagt gaaaaagctg
     1741 gaacgtacgc gcattatgaa cgtcagctta agcggcattc cgctggggga gtggcgcgat
     1801 ttaaccgacg atgagttaat cgacctcttt aagctcatcg aaaattcctc ttccgaggca
     1861 aaacctaaag cgaaggccaa accgaaaaca gcgggcatca aacgtccagt cgttaagatg
     1921 gaaaaaacgg cggaaaaagg cggtcgcccg gcgtccaacg gtaagcgttt tacctcgccg
     1981 gggcgtaaaa agaagggacg ctgattaacg cctcagactg atgaaaacgt ccaaaaagag
     2041 ctcggacagt cccctcgccc cttcggggag agggttaggg tgaggggaag attgtgtcca
     2101 cacagacgag tgaaaatctc aggcgttgat tgttcattag cgcctgccgc gcgtcgacgt
     2161 atttgctgac cagctaaatg acgcttcagg atccggttta taagcctgct ttttcttcaa
     2221 ttggcgggct tttttcgctt cagcctcacg ctcgaccatt aatttatcga tgtactcttt
     2281 cttaatgctg ttggtttcgg agttgttcag tacgcgacca tgcgcaatcc gcgcccgatc
     2341 cagcagtgtt ttaagttcac gctgctcgcg ttcagtcatc tctttttggg taatacgggg
     2401 gagtgccatg atggtgccct cagtgagcca gagagttcag tgtacgggaa ttcgtttgcg
     2461 agcagaactg ataatattca ttttgccttc tcttaactat ttatctcttt cgataattaa
     2521 ttagagatgc agataaaaaa attaaaggca attattctcc gataaagacg ttggttaaca
     2581 gagtacaggc tcgtttattg atgagcatag tgacaagaaa atcaatacgg cccgaaatat
     2641 agcttccagg ccatacagta tttactcaaa caaattacta tgcagttttt gcaccacctg
     2701 ctcggcatct tcgccgggca ccaggaagca caggttatgg ctggatgcgc cgtaacaaat
     2761 catgcgaatg ttgaacggtt ccagtacgcc gaatacctct ttgccaacgc cgcaggcttt
     2821 tgacaggtca ttgccaatca acgcgaccag cgccagacct tcttccacct ccacccggca
     2881 cagtgcggaa agctccatca gcagagattg cgtcagcaac gtatcgccag tggaggttga
     2941 accggtggta tcaagggtta atgccacgct cacttctgac gtggtgatta agtctaccga
     3001 aatattatgc cgcgcgagga tgccgaaaac ttccgcgagg aagccgcgag aatgcagcat
     3061 attcaggctg tgcaaagtga gcagagtctg attgcgacga agcgccagcg cgcggaacag
     3121 cggcggattt tcagttttat tgcacaccag cgtaccacct gcgcgtgggt ctttgctgga
     3181 gccgacaaag accgggatat cgctgcgtac tgcgggtagc aacgttgccg gatgcagtac
     3241 ttttgcacca aaagttgcca tctctgccgc ttcggcaaac gcgatttcat caatgcgttt
     3301 tgctgcggaa actacgcgtg gatcggtggt gtagatgccc gggacatcgg tccagatatc
     3361 aacacgagat gcgtgtaaag cctccgccag caaggctgcc gtataatcgc tgcctccacg
     3421 gccaagcgtc gttgtacgac ctttattttc gctaccgata aatccctggg tgatcactaa
     3481 gccttcattg aggcgtggga gcagctgcag cgcggccagt tccgccagcg cggctacatc
     3541 tggctctgca cgaccaaatc ggtcgttggt acgcatcact ttacgtacat caaaccactg
     3601 tgcctgaaca tcgcgttcgc gcaggatttc gacaaacagc agggtcgaca tcagctcgcc
     3661 gtggctgacc agctcatctg tcagcgccgg agacgttgcc agcgccgccg cttctgccag
     3721 aacagtaatg ttctccagca gacgttcaat ctcttcacgg ataacgttcg ggtaacgcag
     3781 acgttccaga atggcaaact ggatgttgcg aatagcgtcg agtttttcga atcgctcgcc
     3841 aggttccagc ccttccgcta aagcgaccag cagattagtg ataccagcag aagccgagag
     3901 gactactaaa cgcacgttgg catcagaaag cacaatatca gcgctgcggt tcatggcgtc
     3961 aaaatcagct acgctggtac cgccaaattt ggagacaaca atttcagaca taactacctc
     4021 gtgtcagggg atccattttc agccttggca caagggaaga gcggaagacg ggtgggcgca
     4081 gagcgatact tcgctactat tttcacccag aagtgctcca ccacttgcga aacgcccgac
     4141 tgcgaacgct tctggtgaca acccagggga ttcagcccct gtagccgatg atgaacgtgg
     4201 ccagccgttc aatcacctcg gcgatgcacc ccctcaggtg ttatcacagg actggctcct
     4261 ccaacaccgt tacttgggca acgcgcctct tctggcctgc gctagcgcag gtagtacatt
     4321 tataaataaa gggtgagcgg ggcggttgtc aacgacgggg tcatgcggat ttttcatcca
     4381 ctcctggcgg tcagtagttc agctaataaa tgcttcactg cgctaagggt ttacactcaa
     4441 cattacacta acggcactaa aaccatcaca tttttctgtg actggcgcta caatcttcca
     4501 aagtcacaat tctcaaaatc agaagagtat tgctaatgaa aaacatcaat ccaacgcaga
     4561 ccgctgcctg gcaggcacta cagaaacact tcgatgaaat gaaagacgtt acgatcgccg
     4621 atctttttgc taaagacggc gatcgttttt ctaagttctc cgcaaccttc gacgatcaga
     4681 tgctggtgga ttactccaaa aaccgcatca ctgaagagac gctggcgaaa ttacagggtc
     4741 tggcgaaaga gtgcgatctg gcgggcgcga ttaagtcgat gttctctggc gagaagatca
     4801 accgcactga aaaccgcgcc gtgctgcacg tagcgctgcg taatcgtagc aataccccga
     4861 ttctggttga tggcaaagat gttatgccag aagtcaacgc ggtgctggag aagatgaaaa
     4921 ccttctcgga agcgattatc tccggtgagt ggaagggtta taccggcaaa gcaatcactg
     4981 acgtagtgaa catcgggatc ggcggttctg acctcggccc gtacatggtg accgaagcac
     5041 tgcgtccgta caaaaaccac ctgaacatgc actttgtttc taacgtcgat gggactcaca
     5101 tcgcggaagt gctgaaaaaa gtaaacccgg aaaccacgct gttcctggta gcatctaaaa
     5161 ctttcaccac tcaggaaacc atgaccaacg cccatagcgc gcgtgactgg ttcctgaaag
     5221 cggcaggtga tgaaaaacac gttgcgaaac attttgcggc gctctcaacc aatgccaaag
     5281 ccgttggcga gtttggtatt gatactgcca acatgttcga gttctgggac tgggtcggcg
     5341 gccgttactc tttgtggtca gcgattggcc tgtcgattgt tctctccatc ggctttgata
     5401 acttcgttga actgctttct ggcgcacacg caatggacaa gcatttctcc accacgcctg
     5461 ccgagaaaaa cctgcctgtc ctgctggcgc tgattggcat ctggtacaac aatttctttg
     5521 gtgcggaaac tgaagcgatt ctgccgtatg accagtatat gcaccgtttc gcggcgtact
     5581 tccagcaggg caatatggag tccaacggta agtatgttga ccgtaacggt aacgttgtgg
     5641 attaccagac tggcccgatt atctggggtg aaccaggcac taacggtcag cacgcgttct
     5701 accagctgat ccaccaggga accaaaatgg taccgtgcga tttcatcgcc ccggctatca
     5761 cccataaccc gctctctgat caccaccaga aactgctgtc taacttcttc gcccagaccg
     5821 aagcgctggc gtttggtaaa tcccgcgaag tggttgagca ggaatatcgc gatcagggta
     5881 aagatccggc aacgcttgac tacgtggtgc cgttcaaagt gttcgaaggt aaccgcccga
     5941 ccaactccat cctgctgcgc gaaatcactc cgttcagcct gggtgcgttg attgcgctgt
     6001 atgagcacaa aatctttact cagggcgtga tcctgaacat cttcaccttc gaccagtggg
     6061 gcgtggagct gggtaaacaa ctggcaaacc gtattctgcc agagctgaaa gatgataaag
     6121 aaatcagcag ccacgatagc tcgaccaatg gtctgattaa ccgctataaa gcgtggcgcg
     6181 gttaatcatc atgcgtgcct gatgccgata tgtaggccgg ataaggcgtt tacgccgcat
     6241 ccggcaaccg gagcctgatg ccaatatcga cattaatgca tagtatgagg agttaaataa
     6301 ccttaaaaac aatgccgaca ataagtcggc attgttttat cagataaatc cccttgtctg
     6361 tgatttaacg gaaatcatac cgtgaggtta atcctaaaat agatttttaa tcgttgttta
     6421 tttcggagaa tacgcagatt aattactttt gtttttattt taattttatg atttttattg
     6481 ttatttaaat ataagttgaa acttatattt gatattcatt ccaattatcc taaaactcca
     6541 tcgctaattc cccgcgccgt aattcgcatg ctttagttgt gtatactcga tcccgcccga
     6601 aatgtttttg ggtaaatctc tattcattca atgaagggaa attgttatga aaaaagttct
     6661 gtatggcatt tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg cacctgtaca
     6721 ggtgggcgaa gcggcagagt cggcagcaac gtcggtttcg gcggggagtt cctccgcgac
     6781 cagcgtcagc accgtaagct cggcggtggg tgtcgcgctc gcggcaaccg gtggcggtga
     6841 tggttctaat accgggacca ccacaaccac aaccacgacc accagtaccc agtaataaag
     6901 tatgtatccc caaaataatt tgagtcattg catctgtggc ttgaagtatg aagggattta
     6961 ccataaccac actccggtgt ggttattctg cccctctgga gaagagtcgt gaagcgacct
     7021 gcactcattc ttatctgcct gctattacag gcctgttcag ccacgactaa agagctgggc
     7081 aattcactgt gggacagtct gttcggcacg ccaggcgtac agctgacgga cgatgatatt
     7141 caaaatatgc cctacgccag ccagtacatg cagcttaatg gcgggccgca attgtttgtg
     7201 gtgctggcct tcgctgaaga cggacaacaa aaatgggtca ctcaggatca agccactctt
     7261 gtcacacaac atggtcgtct ggtgaagact ttgcttggcg gcgacaacct gattgaagtg
     7321 aataaccttg ccgccgaccc gctgattaaa cccgcacaaa ttgttgatgg tgcaacatgg
     7381 acgcgcacga tgggctggac cgagtaccag caggtacgct acgccaccgc acgctcagtc
     7441 ttcaaatggg atggcaccga taccgtcaag gtcggcagcg atgaaaccct ggttcgcgtg
     7501 ctggacgaag aagtctccac cgaccaggcg cgctggcata accgctactg gatcgacagc
     7561 gaagggcaaa ttcgccagtc ggaacagtat ctcggcgcgg attatttccc ggtgaaaacc
     7621 actctcatca aggcggcaaa acaatgatta aacaaactat tgtcgcgttg cttttgagtg
     7681 tgggagcgtc atcggtcttc gcggcaggaa ccgtcaaggt gttcagcaat ggcagcagtg
     7741 aggccaaaac gctaacgggc gcagagcatt taatcgatct ggtagggcaa cctcgactgg
     7801 caaacagctg gtggcctggt gcggtgatta gcgaagagct ggcaacggcg gcagcattgc
     7861 gtcagcagca ggcgttgctg acacggctgg cagaacaggg cgcagattcc agcaccgacg
     7921 atgccgccgc cattaacgcc ttacgccagc aaatccaggc attgaaagtg acaggcaggc
     7981 aaaaaatcaa tcttgatccc gatatcgtcc gcgttgccga acgcggtaac ccgccgttgc
     8041 agggcaacta cacgctgtgg gtcggaccac cgccgtccac ggtcacgttg ttcgggctta
     8101 tcagccatcc tggcaatcag ccattcactc caggtcgcga cgtggcgagc tatctctctg
     8161 accagagcct gctcagcggt gcggatcgca gctacgcgtg ggtcgtttac ccggacggac
     8221 gcacgcaaaa agcgccggtg gcttactgga acaagcgtca cgtagagccg atgcccggca
     8281 gcattattta tgttggcctc gcagactccg tctggagtga gacgcctgat gcccttaacg
     8341 ccgacattct tcagactctg acgcagcggg taccccaata atgaaaaaaa gacatctgct
     8401 tagcttactg gcgctgggca ttagcacagc ttgctacggc gaaacatatc ctgcgcccat
     8461 tggtccgtcg cagtcggatt tcggtggcgt aggattatta caaactccca ccgcgcgtat
     8521 ggcacgggaa ggggagttga gtctgaacta tcgcgataac gatcagtacc gttattactc
     8581 agcttcagtg caactcttcc cgtggctgga aacaacgctg cgctacaccg acgtgcgcac
     8641 ccggcagtac agcagcgtcg aagcgttctc tggcgatcaa acgtataaag ataaagcctt
     8701 cgatctcaaa ctgcgtttgt gggaagagag ttactggctg ccgcaagtgg cggttggtgc
     8761 gcgggatatc ggcggtacgg ggctgtttga tgcggaatat cttgttgcca gcaaagcctg
     8821 ggggccgttc gattttacgc tcggcctggg ctggggatat ctgggcacca gcggtaatgt
     8881 aaaaaatccg ctctgttcag ccagtgataa atattgctat cgcgataaca gctacaaaca
     8941 ggcgggatct atcgacggta gccagatgtt ccacggtcct gcctcactgt ttggcggcgt
     9001 ggaataccag acgccctggc aaccgctgcg tctgaaactg gagtatgaag gtaataatta
     9061 tcagcaggat tttgccggga agctggagca aaaaagtaag tttaacgtcg gggcgattta
     9121 tcgtgttacc gattgggccg acgttaacct tagctatgaa cgtggcaaca cctttatgtt
     9181 tggcgtcacg ctgcgcacca actttaacga tctgcgcccg tcctacatcg ataacgcccg
     9241 cccgcaatat caaccgcagc cgcaggatgc cattttgcag cattcggtgg tggcgaatca
     9301 gttaacgttg ctgaaataca acgccggact tgccgatccg cagatccagg cgaaaggcga
     9361 tacgctgtat gttaccggcg agcaggtgaa atatcgtgat tcgcgcgaag ggatcatccg
     9421 tgctaatcgg atcgtgatga acgatctgcc ggatgggatc aaaacgatcc gcattacgga
     9481 aaatcgcctt aacatgccgc aagtgacgac ggaaaccgat gtcgccagcc tgaaaaatca
     9541 tctcgccgga gagccgttgg gccacgaaac gacgctggcg cacaaacgcg tcgagccagt
     9601 ggttccgaag tccaccgagc agggctggta tatcgacaaa tcacgctttg atttccatat
     9661 cgatccggtg ctgaaccagt cggtcggtgg cccggaaaac ttttacatgt atcagctggg
     9721 cgtgatggga acggcagatt tgtggctgac ggaccatcta ctgaccaccg gcagcctgtt
     9781 tgcaaatctt gccaacaact acgacaagtt taactacacc aatccaccgc aggactcgca
     9841 cttaccgcgc gttcgtaccc atgtgcgcga gtatgtgcag aacgatgtct atgtgaataa
     9901 cctgcaagcc aactacttcc agcatctggg caacggcttc tacggtcagg tctatggagg
     9961 ttatctcgaa accatgtttg gcggtgcggg ggccgaagtg ttgtatcgtc cgctggacag
    10021 taactgggcg tttggtctgg atgccaacta cgttaaacag cgcgactggc gtagtgcaaa
    10081 agatatgatg aaattcaccg actacagcgt gaaaaccgga catctgaccg cctactggac
    10141 gccatctttc gcccaggacg tgttggtgaa agccagcgtc gggcagtatc tggcagggga
    10201 taaaggcggc acgctggaga tcgctaaacg ctttgacagc ggcgtggtgg tgggtggcta
    10261 tgccacgatc accaacgttt cgaaagagga gtacggcgaa ggggacttca ccaaaggcgt
    10321 gtatgtctcg gtaccgttgg atctcttctc gtctggcccg acacgcagcc gtgcggcgat
    10381 tggctggacg ccgctgacgc gtgacggtgg tcagcaactt gggcgtaagt tccagttgta
    10441 tgacatgacc agcgaccgta gcgtaaattt ccgctaagtc atgggaaagg tgccagtttt
    10501 cgcactcact ggcaccctca ttcttaatga cctgttgagc catcaggaac gcagttcacc
    10561 cccggtttac gatctggtgt gtaaaggcga aaacattctg tcttcctgtc cgtcgatatc
    10621 ggttgcaggt ctggttgcct gagcgactgg gccatcattg ggctggagac attgctttgc
    10681 ggtaggtcgg ctttatcagc agtaccgagc ggaccagcat aagcaggaag aacagagact
    10741 gataacatca aagcagcaaa ataaggcttc atttttacca cctttatcag gttacgtttc
    10801 atttgttcca gaggatcatt gtcgattttt cgcgcattgc tggtggctgg gaatcacctg
    10861 aatgggtgat ttttgaatta ccggctttgg tgcggtttgt cttgccggat gcgccgccag
    10921 gcgcggctta tccggcctac gggtaggtat atccggcttt ggtggaggcg cgctccaaat
    10981 ccaggttgaa caaaacatac acaaaaaata tagatctccg tcacattttt gcgttataca
    11041 ggaagctcgc cactgtgaag gaggtactgc tatgacgtca ctctctcgtc cgcgcgtgga
    11101 gtttatctcc accattttgc agaccgtact caatcttggc ctgctgtgcc tcggcctgat
    11161 tttggttgtc ttcctcggca aagaaacggt gcatctggct gatgtgctgt tcgcgccaga
    11221 acaaaccagc aaatatgagc tggtagaagg actggtggtt tactttctct atttcgaatt
    11281 tatcgcgctg attgtgaagt actttcagtc cggttttcac ttcccgctac gttactttgt
    11341 ctatattggg atcaccgcca ttgtgcgctt gatcatcgtc gatcataaat cgccactgga
    11401 tgtgttgatc tactcggctg cgatcctgtt actggtgatc accctgtggc tgtgtaattc
    11461 gaaacggctg aagcgggagt aaaaagacag cacgccgaaa tggcgcggcg tgctggacag
    11521 gaagattaca gcgtagcagt ttgttgtgtt ttcttcgttt ccggttccca gagcgcttcc
    11581 agctcctcaa gggttttacc tttggtttcc gggacaaatt tccacataaa cagtgctgcc
    11641 agaacgccca tacaaccgta aatccagtag gagaaaccgt tgtggaaatg ggccaccagc
    11701 caggagtttt tgtccatcat cgggaaggtc caggagacga agtagttcgc cagccactgg
    11761 gccgccaccg cgattgccag cgctttacca cgaatagcat tcgggaagat ttccgacagc
    11821 agtacccagc ataccggacc ccaggacatg gcaaaggcgg caacatagaa cagcatcgac
    11881 agtagcgcca caatacccga tgcctgagtg taaaacgcgg taccgaggct aaacataccg
    11941 attgccattc cgagtgcgcc gataatttgc agtggcttac gaccaaattt atccacagtc
    12001 ataattgcca gtacggtaaa ggtgaggttg ataactccga caataatggt ctgcaacagc
    12061 gcgatatccg tgctggcccc cagcgttttg aacacttccg gcgcgtagta cagcaccaca
    12121 ttgatgccga caaattgctg gaagatggag agcataacgc cgattacaat cacgcccacg
    12181 ccaaacatca gcagacgacc accggttttg cggccatgat ccagggagtg tttaatttcc
    12241 tgtactgcct gagttgcaag cgtgttgccc ataattttgc gcaggatact ttccgcctgt
    12301 tcttgcttgc cgcgtgacat cagccagcga ggactttctg gcacggtata cagcagcatt
    12361 aagaacagca gtgcagggat acattccgag gcaaacatat aacgccagcc gtcagtattc
    12421 agccagctgg catcaccgga acgggcaata aaatagttta cgcagtaaac taaaagttgc
    12481 ccgaaaataa tcgcaaactg gttaaatgaa accagttttc cgcgaatatg agctggagcc
    12541 agttccgcaa tatacattgg tgaaagcatt gaggctaaac caacgccaat accgccaata
    12601 atacgataaa taacaaattc cgggacataa cctgccagat aaataggtac tgtgctgtcc
    12661 gggtttatag aggtaaaacc aagttctggc caggcagaac ctacaccaga aataaaaaac
    12721 aggacagcag aaatcttaag tgaatcacga cggccgaagc ggttactgca ataaccaccg
    12781 agggcaccgc cgatgatgca accaatcaga gcgctggcta cgcaaaatcc taacagggag
    12841 ttggcagcgg attcacttaa gttttgtgga gcaacaaaga cggtattgag tgactcaaca
    12901 gtaccggaaa taacggcggt gtcgtagcca aataataaac cacctaatgt agcgactaag
    12961 gtaatcgaaa atatataact ggaattatac tgggtattca ttcagacctg ccttagacca
    13021 ttctgatctt ttatcagtgc attgatgttg gaattgtcag gcagttaagg caattatcat
    13081 tttttacaat gcgtttacgt aatgtctttt ctgtgatctt aattgtgata attatcaaaa
    13141 attgatgaaa acaatactat tgccgtgact cagagcacga aagagaatta tcgtaagtgg
    13201 gaaaacaaat aacgtaaaaa taataagttc tatgatggga atattaaccg gcgaacgatt
    13261 cagattgcag acgaaagaaa aaaaggcgct ccgtggagcg ccgaataata gtcacaagtt
    13321 gggataacgt aagttgaggg tgcagcggca taacattggc agaacaacat ctttaacctt
    13381 tcacaccacc tgccgtcagg ccgttgacca gccagcgttg agccagcaag aagacgatgg
    13441 tgatcggtaa tgcagacatc acggcagcgg cggcaaagtc accccacagg tagttttgcg
    13501 ggttgaggta ttgctgcatc cccacggcca gggtgtagct gtttacgtca cgcagtaaca
    13561 gcgacgcgac cggaacttca gtaatggcag cgataaacga caggatgaat accaccgcca
    13621 gaatcggtac tgacagcggc aacaggacaa ggcggaaggc ctgccacggt gtcgcaccat
    13681 ccagcgcagc cgcttcttcc agcgaactgt cgatggtttc gaaatagcct ttgatggtcc
    13741 agacatgcag cgcaatccca cccagatacg cgaaaattac gccgccgtga gtattcaggc
    13801 caatgaatgg aatgtactca cccagacgat caaacaacgc atacaacgcg accagtgaaa
    13861 gtactgccgg gaacatctgg aaaatcagca ttcctttcag cagcgtcgct ttgcctggaa
    13921 agcgcatacg ggcgaaagcg taggcgcagg tggtggagag cgccacaatg ccaatcgcgg
    13981 aaatcccagc gacctttacc gagttccaca gccacagcag taccgggaat ggcggtggcg
    14041 taatgcgacc atcagcctgt tcaacgctaa aacctaacgc cagtttccag tgatcccagg
    14101 agatttgctc cgggatcagg ctaccggtcg cgaagtttcc ctgacgcagc gagatagcga
    14161 cgaccatcag cagcgggaac ataatcgccg cgataaaaag tagcagtagc aggtgagtaa
    14221 taaacaaacg tgctttttgc gatttcggtt ggaccattgc catttttgtt atctccctta
    14281 atcaaacttc attcgcgtgg ctttcaggtt cactatcgcc agcgcaccca ccagcaggaa
    14341 gatcagcgtg gcaattgctg ccgccagacc gaagtcctga cccccgccgc cttcaaaagc
    14401 gatacggtag gtgtagttaa caagcaggtc ggtataaccg gctggcgtgg tcgtgccaag
    14461 acgatccggg ccgccgttgg ttaacagctg aatcagcacg aagttgttaa agttaaaggc
    14521 gaagctggcg atcatcagcg gcgtcagcgg tttaatcagc agcggcagcg taatcttaaa
    14581 gaagttctgg aacggacctg cgccatccat tgctgaggct tcatacaaat cgtccggaat
    14641 cgctttcagc aagcccatgc agaggatcat catgtacgga taacccagcc aggtattgac
    14701 gataatcagc atcgtgcgag cggtggtcgg atcgctgaac caggcgggct tcacgccaaa
    14761 cagcgcgctc aacatcatgt tgatttcacc gaagctctgg ttaaacaatc ctttgaaaat
    14821 caagattgaa atgaacgatg gcaccgcgta gggcagaatc agcaggacgc gatagaccgc
    14881 tttgccgcgc aacgcttccc actgcaccag acatgccaga accatgccga ccgccaccgt
    14941 taaaaagaca gtgatcagcg agaacaccac ggtccagacg aaaatggcga ggaacggttt
    15001 ctgaatgcct tcgtcggtaa agacgcgggt aaagtttttc cagccggtgg tcacggtgta
    15061 accggggctt agcttttcat ccccccagtt gccgtcggcg gtaatggact ggtaaaagcc
    15121 aatttggtta ttcggacgat atttcacgcc actctgatta ttcgtcaacg tgccgtcacc
    15181 gtcgagtgtg tagagcggct gcgtgccaga aaactggcgc agggagctca tcatcacttt
    15241 gttgccatcc ggcagaatgg tggtaatgtc actcagcgcc tgacgattct gggtaatcac
    15301 gcgcagattc gcgcgttcgc cttcgggctg ggcggtcgtt tctttcagtt gcagtttttg
    15361 ctcgccgcca aatttaaacg cgtcggagag gtaatttttg ccggtttcgc cgtcgctgag
    15421 cgccagttgc cactcatcgc ccgccgggta aagaccaaag ttataggttt tgcctgcttg
    15481 ccaggagcga tctaacaaca cttcctgcgc acgttcaaaa gtcagctggt tagtgctgct
    15541 gtagttggtg aaggcaatgg cgatggtgca gaccagaggg aagaggacga ataatcccat
    15601 tccagccatt cccgggtaaa catagcgcca ggcgtaggct ttacgattgg cgaaaatata
    15661 cagccccgct gaactcaata tcagcgtggt aatggcgaac aggtattccc cttgtgcgta
    15721 cattaaaaca acaaggtaac ccaccagcag gccgagcaga cctagcactg accatttcag
    15781 cgcgtcgctt tgccaccaat gtttcttttt aatgacatcc atggggttct tcctcattcc
    15841 aggacggata aggctttcac gccttatccg acaacaactg cctgatgcga cactgatgcg
    15901 tcttatcagg cctacatgcg tttcagtttt gtaggccgga caaggcgttc acgccgcatc
    15961 cggcatttca cagcattact tggtgatacg agtctgcgcg tctttcaggg cttcatcgac
    16021 agtctgacga ccgctggcgg cgttgatcac cgcagtacgt acggcatacc agaaagcgga
    16081 catctgcggg atgttcggca tgatttcacc tttctgggcg ttttccatgg tggcggcaat
    16141 acgtggatct ttcgccaact cttcctcgta agacttcagc gctacggcac ccagcggttt
    16201 gtctttatta accgcttcca gaccttcatc agtcagcaga tagttttcga ggaactcttt
    16261 cgccagctct ttgttcggac tggcggcgtt aataccggcg ctcagcacgc caacgaacgg
    16321 tttagatggt tgacccttga aggtcggcag taccgttaca ccataattca ctttgctggt
    16381 gtcgatgttg gaccatgccc acgggccgtt gatggtcatc gctgtttcgc ctttattaaa
    16441 ggcagcttct gcgatggagt aatcggtgtc tgcattcatg tgtttgtttt taatcaggtc
    16501 aaccaggaag gtcagacccg ctttcgcgcc agcgttatcc acgcccacgt ctttaatgtc
    16561 gtacttgccg ttttcatact taaacgcata acccccgtca gcagcaatca gcggccaggt
    16621 gaagtacggt tcttgcaggt tgaacatcag cgcgctctta cctttcgctt tcagttcttt
    16681 atccagcgcc gggatctctt cccaggtttt tggcgggttc ggcagcagat ctttgttata
    16741 aatcagcgat aacgcttcaa cagcgatcgg gtaagcaatc agcttgccgt tgtaacgtac
    16801 ggcatcccat gtaaacggat acagcttgtc ctggaacgct ttgtccgggg tgatttcagc
    16861 caacaggcca gattgagcgt agccaccaaa gcggtcgtgc gcccagaaga taatgtcagg
    16921 gccatcgcca gttgccgcaa cctgagggaa tttctcttcc agtttatccg gatgctcaac
    16981 ggtgacttta attccggtat ctttctcgaa tttcttaccg acttcagcga gaccgttata
    17041 gcctttatcg ccgttaatcc agattaccag tttaccttct tcgattttgg cgagagccga
    17101 ggcggaaaac atcatcgtcg ttaatgcgga taatgcgagg atgcgtgcac ctgtttttat
    17161 tttcataatc tatggtcctt gttggtgaag tgctcgtgaa aacacctaaa cggactctag
    17221 tttctttata cggcaacctc tttccatcct ccttgcccct acgccccacc gttgctttgt
    17281 gtgatctctg ttacagaatt ggcggtaatg tggggatgcg cacataaaat cgccacgatt
    17341 tttgcaagca acatcacgaa attccttaca tgacctcggt ttagttcaca gaagccgcgt
    17401 tctcatcctc ccgcctcctc ccccataaaa aagccagggg gtggaggatt taagccatct
    17461 cctgatgacg catagtcagc ccatcatgaa tgttgctgtc gatgacaggt tgttacaaag
    17521 ggagaagggc atggcgagcg tacagctgca aaatgtaacg aaagcctggg gcgaggtcgt
    17581 ggtatcgaaa gatatcaatc tcgatatcca tgaaggtgaa ttcgtggtgt ttgtcggacc
    17641 gtctggctgc ggtaaatcga ctttactgcg catgattgcc gggcttgaga cgatcaccag
    17701 cggcgacctg ttcatcggtg agaaacggat gaatgacact ccgccagcag aacgcggcgt
    17761 tggtatggtg tttcagtctt acgcgctcta tccccacctg tcagtagcag aaaacatgtc
    17821 atttggcctg aaactggctg gcgcaaaaaa agaggtgatt aaccaacgcg tcaaccaggt
    17881 ggcggaagtg ctgcaactgg cgcatttgct ggatcgcaaa ccgaaagcgc tctccggtgg
    17941 tcagcgtcag cgcgtggcga ttggccgtac gctggtggcc gagccaagcg tatttttgct
    18001 cgatgaaccg ctctccaacc tcgatgctgc actgcgtgtg caaatgcgta tcgaaatctc
    18061 ccgtctgcat aaacgcctgg gccgcacaat gatttacgtc acccacgatc aggtcgaagc
    18121 gatgacgctg gccgacaaaa tcgtggtgct ggacgccggt cgcgtggcac aggttgggaa
    18181 accgctggaa ctgtaccact atccggcaga ccgttttgtc gccggattta tcggttcgcc
    18241 aaagatgaac ttcctgccgg taaaagtgac cgccaccgca atcgatcaag tgcaggtgga
    18301 gctgccgatg ccaaatcgtc aacaagtctg gctgcctgtt gaaagccgtg atgtccaggt
    18361 tggagccaat atgtcgctgg gtattcgccc ggaacatcta ctgccgagtg atatcgctga
    18421 cgtcatcctt gagggtgaag ttcaggtcgt cgagcaactc ggcaacgaaa cccaaatcca
    18481 tatccagatc ccttccattc gtcaaaacct ggtgtaccgc cagaacgacg tggtgttggt
    18541 agaagaaggt gccacattcg ctatcggcct gccgccagag cgttgccatc tgttccgtga
    18601 ggatggcact gcatgtcgtc gactgcataa ggagccgggc gtttaagcac cccacaaaac
    18661 acacaaagcc tgtcacaggt gatgtgaaaa aagaaaagca atgactcagg agatagaatg
    18721 atgattactc tgcgcaaact tcctctggcg gttgccgtcg cagcgggcgt aatgtctgct
    18781 caggcaatgg ctgttgattt ccacggctat gcacgttccg gtattggctg gacaggtagc
    18841 ggcggtgaac aacagtgttt ccagactacc ggtgctcaaa gtaaataccg tcttggcaac
    18901 gaatgtgaaa cttatgctga attaaaattg ggtcaggaag tgtggaaaga gggcgataag
    18961 agcttctatt tcgacactaa cgtggcctat tccgtcgcgc aacagaatga ctgggaagct
    19021 accgatccgg ccttccgtga agcaaacgtg cagggaaaaa acctgatcga atggctgcca
    19081 ggttccacca tctgggcagg taagcgcttc taccaacgtc atgacgttca tatgatcgac
    19141 ttctactact gggatatttc tggtcctggt gccggtctgg aaaacatcga tgttggcttc
    19201 ggtaaactct ctctggcagc aacccgctcc tctgaagctg gtggttcttc ctcttttgcc
    19261 agcaacaata tttatgacta taccaacgaa accgcgaacg acgttttcga tgtgcgttta
    19321 gcgcagatgg aaatcaaccc gggcggcaca ttagaactgg gtgtcgacta cggtcgtgcc
    19381 aacctgcgtg ataactatcg tctggttgat ggcgcatcga aagacggctg gttattcact
    19441 gctgaacata ctcagagtgt cctgaagggc tttaacaagt ttgttgttca gtacgctact
    19501 gactcgatga cctcgcaggg taaaggtctg tcgcagggtt ctggcgtcgc gtttgataac
    19561 gaaaaatttg cctacaatat caacaacaac ggtcacatgc tgcgtatcct cgaccacggt
    19621 gcgatctcca tgggcgacaa ctgggacatg atgtacgtgg gtatgtacca ggatatcaac
    19681 tgggataacg acaacggcac caagtggtgg accgttggta ttcgcccgat gtacaagtgg
    19741 acgccaatca tgagcaccgt gatggaaatc ggctacgaca acgtcgaatc ccagcgcacc
    19801 ggcgacaaga acaatcagta caaaattacc ctcgcacaac aatggcaggc tggcgacagc
    19861 atctggtcac gcccggctat tcgtgtcttc gcaacctacg ccaagtggga tgagaaatgg
    19921 ggttacgact acaacggcga tagcaaggtt aacccgaact acggcaaagc cgttcctgct
    19981 gatttcaacg gcggcagctt cggtcgtggc gacagcgacg agtggacctt cggtgcccag
    20041 atggaaatct ggtggtaata gcaaaacctg ggccggataa ggcgtttacg ccgcatccgg
    20101 cataaaaaca ggttgtcatt atctgaaagg ggcgaaagcc cctctgatta tcgggtttag
    20161 cgcgctattg cctggctacc gctgagctcc agattttgag gtgaaaacaa tgaaaatgaa
    20221 taaaagtctc atcgccctct gtttatcagc agggttactg gcaagcgcgc ctggaattag
    20281 ccttgccgat gttaactacg taccgcaaaa caccagcgac gcgccagcca ttccatctgc
    20341 cgcgctgcaa caactcacct ggacaccggt cgatcaatct aaaacccaga ccacccaact
    20401 ggcgaccggc ggccaacaac tgaacgttcc cggcatcagt ggtccggttg ctgcgtacag
    20461 cgtcccggca aacattggcg aactgaccct gacgctgacc agcgaagtga acaaacaaac
    20521 cagcgttttt gcgccgaacg tgctgattct tgatcagaac atgaccccat cagccttctt
    20581 ccccagcagt tatttcacct accaggaacc aggcgtgatg agtgcagatc ggctggaagg
    20641 cgttatgcgc ctgacaccgg cgttggggca gcaaaaactt tatgttctgg tctttaccac
    20701 ggaaaaagat ctccagcaga cgacccaact gctcgacccg gctaaagcct atgccaaagg
    20761 cgtcggtaac tcgatcccgg atattcccga tccggttgct cgtcatacca gcgatggctt
    20821 actgaaactg aaagtcaaaa cgaactccag ctccagcgtg ttggtaggac cactatttgg
    20881 ttcttccgct ccagctccgg ttacggtagg caacacggcg gcaccagctg tggctgcacc
    20941 tgctccggtg ccggtgaaga aaagcgagcc gatgctcaac gacacggaaa gttattttaa
    21001 taccgcgatc aaaaacgctg tcgcgaaagg tgatgttgat aaggcgttaa aactgcttga
    21061 tgaagctgaa cgcctgggat cgacatctgc ccgttccatc tttatcagca gtgtaaaagg
    21121 caaggggtaa ttacgcccca cagtgctgat tttgcaacaa ctggtgcgtc tcctggcgca
    21181 ccttttttta tgcttccttc ctgggatatg agcgattttt tatagtactt cacttcttct
    21241 tcactaagaa tgtccattat ttcaatgcat tatcagagat tcttttcctt tcgccggtag
    21301 tgtctggacg ctcaggctac ttttccaggt tattttattt ctgtattaca gaggttttat
    21361 gataggtcat atcacaaatt ctggcggcaa taactctttg atgaaacatg atgtggtgca
    21421 aggaaataat atagtagatc ttgatttact acgtaatttc aatggggtga caggtttaaa
    21481 cagagataac tttatttata tcagtgatat atttttaaat ataaaacaac ggaacgaaaa
    21541 aaatcatgca ataaatatgt tacgtgaagt ttcaattagt aatgatacta taagtgtaaa
    21601 attctacaga aatgaaaaaa tagaatgcgc ttgtaatttt ctgatggata aagatgcgca
    21661 ggggtatatc gacctgtctg attttgattt aacaaattgt cattttaaag gtgacgttat
    21721 ttctaaggtg t
//
LOCUS       DBKEKI010000058        21334 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.058, whole genome shotgun sequence.
ACCESSION   DBKEKI010000058 DBKEKI010000000
VERSION     DBKEKI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.058"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            66..992
                     /locus_tag="ACUQPX_002228"
                     /pseudo
     CDS             66..992
                     /locus_tag="ACUQPX_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
     gene            1231..1290
                     /locus_tag="ACUQPX_002229"
     CDS             1231..1290
                     /locus_tag="ACUQPX_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HHV9056933.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1607..1666
                     /gene="ibsE"
                     /locus_tag="ACUQPX_002230"
     CDS             1607..1666
                     /gene="ibsE"
                     /locus_tag="ACUQPX_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HHV9056934.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1786..3219)
                     /gene="hldE"
                     /locus_tag="ACUQPX_002231"
     CDS             complement(1786..3219)
                     /gene="hldE"
                     /locus_tag="ACUQPX_002231"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HHV9056935.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3267..6107)
                     /gene="glnE"
                     /locus_tag="ACUQPX_002232"
     CDS             complement(3267..6107)
                     /gene="glnE"
                     /locus_tag="ACUQPX_002232"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HHV9056936.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SRLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6130..7431)
                     /gene="ygiF"
                     /locus_tag="ACUQPX_002233"
     CDS             complement(6130..7431)
                     /gene="ygiF"
                     /locus_tag="ACUQPX_002233"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HHV9056937.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGSIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7673..8293
                     /locus_tag="ACUQPX_002234"
     CDS             7673..8293
                     /locus_tag="ACUQPX_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HHV9056938.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8357..9595
                     /gene="cca"
                     /locus_tag="ACUQPX_002235"
     CDS             8357..9595
                     /gene="cca"
                     /locus_tag="ACUQPX_002235"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HHV9056939.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9758..10579)
                     /gene="bacA"
                     /locus_tag="ACUQPX_002236"
     CDS             complement(9758..10579)
                     /gene="bacA"
                     /locus_tag="ACUQPX_002236"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HHV9056940.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFTPFAIYRFIVAAAVYVVFF"
     gene            complement(10670..11038)
                     /gene="folB"
                     /locus_tag="ACUQPX_002237"
     CDS             complement(10670..11038)
                     /gene="folB"
                     /locus_tag="ACUQPX_002237"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HHV9056941.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11143..11760
                     /gene="plsY"
                     /locus_tag="ACUQPX_002238"
     CDS             11143..11760
                     /gene="plsY"
                     /locus_tag="ACUQPX_002238"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HHV9056942.1"
                     /translation="MSAIALGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11773..12705)
                     /gene="ttdR"
                     /locus_tag="ACUQPX_002239"
     CDS             complement(11773..12705)
                     /gene="ttdR"
                     /locus_tag="ACUQPX_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HHV9056943.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPERMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            12912..13755
                     /gene="ttdA"
                     /locus_tag="ACUQPX_002240"
                     /pseudo
     CDS             12912..13755
                     /gene="ttdA"
                     /locus_tag="ACUQPX_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13752..14357
                     /gene="ttdB"
                     /locus_tag="ACUQPX_002241"
     CDS             13752..14357
                     /gene="ttdB"
                     /locus_tag="ACUQPX_002241"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HHV9056944.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14405..15868
                     /gene="ttdT"
                     /locus_tag="ACUQPX_002242"
     CDS             14405..15868
                     /gene="ttdT"
                     /locus_tag="ACUQPX_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HHV9056945.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLLIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15911..16924)
                     /gene="tsaD"
                     /locus_tag="ACUQPX_002243"
     CDS             complement(15911..16924)
                     /gene="tsaD"
                     /locus_tag="ACUQPX_002243"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HHV9056946.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17162..17377
                     /gene="rpsU"
                     /locus_tag="ACUQPX_002244"
     CDS             17162..17377
                     /gene="rpsU"
                     /locus_tag="ACUQPX_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HHV9056947.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17488..19233
                     /gene="dnaG"
                     /locus_tag="ACUQPX_002245"
     CDS             17488..19233
                     /gene="dnaG"
                     /locus_tag="ACUQPX_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HHV9056948.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19428..21269
                     /gene="rpoD"
                     /locus_tag="ACUQPX_002246"
     CDS             19428..21269
                     /gene="rpoD"
                     /locus_tag="ACUQPX_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HHV9056949.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5141 a         5893 c         5484 g         4816 t
ORIGIN      
        1 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca
       61 ttaccgaaca acaagaagcg tttatgacgc ttgagcagga gcagcaggtt aaaacccgta
      121 ctgccgaaca gaatgcacgt attgcggctt ttgaagctga acgtcgtcgt gaagcagagc
      181 agacgcgaat tctggctgaa cgacaaattc aggaaacgga gatcgagcgc gagcaggccg
      241 tccgctcaag aaaggttgag gctgaacgtg aagttcgtat taaagagatc gaacagcagc
      301 aggtcaccga aatcgccaac cagacgaaat cgatcgctat tgccgccaaa tcggaacaac
      361 agtcccaggc agaagcgcgt gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa
      421 acgtagaaac cactcgccag accgcagaag ccgatcgtgc taaacaagtt gccctaatcg
      481 ctgccgcgca ggatgcagaa accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa
      541 aagaagccgc agagatgcag gcggcggcta tcgttgagtt agccgaagct acacgtaaaa
      601 agggtctggc ggaagcagaa gcacaacgtg cgctgaacga tgctatcaac gtactttctg
      661 atgaacaaac cagccttaaa ttcaaactgg ccttgttgca ggcgctacct gcggtaatag
      721 aaaaatccgt tgagccgatg aaatctattg acggtatcaa gattattcag gtcgatggtc
      781 tgaatcgtgg cagcgctgcg ggtgatgcaa acacgggtaa tgtggggggc ggaaacctag
      841 cggaacaagc attatcagcc gctctctctt accgcacaca ggcaccgctg attgactcct
      901 tgctcaatga aattggcgtt tcaggcggtt cactggcggc attgacttca cccttaacct
      961 caacaactcc cgtcgccgaa aacgtagaat aaattttcag ctatgaacgt ccctagcaca
     1021 aaaagccggg gattttttat atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg
     1081 caacgttttg cgtgaccgct cggggaaggc aaaactgcct ctgggaaagc gttgcgcgag
     1141 gtccggcgct catcaacaat cggggagtca gcaaagggct gaaacgggaa agcccctccc
     1201 gaggaagggg ccttacagaa ggaaagggtt atgatgaagc tcgtcatcat actgattgtg
     1261 ttgttactcg taagtttcgc agcttattaa cagccaatca gaggggggag aaatcctccc
     1321 tcacccttgt tcctttactt taggttgaaa aaaaatcagc gtcaagaagg tttcattaac
     1381 acatatccct ggcccaaaaa gccggggatt ttttatatct gcgttccgct aaaaggtgca
     1441 aatgctcagg acgttgcagc gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg
     1501 gaaagcgttg cgcggggtcc ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa
     1561 cgggaaagcc cctcccgagg aaggggcctt atagaaggaa agggttatga tgaagttcgt
     1621 catcatactg gttgtgttgt tactgttaag tttcccgact tactaacaac tgatcagagg
     1681 ggggagaaat cctcccttac ccttgttcct ttactttagg ttgaaaaaac aacagcgtca
     1741 ataggcctgc ctgctacgaa gcgagatctg tgaaccgctt tccggttagc cttttttatc
     1801 ctgttggatc ttcttgatga tgttagtggt cgagcaaccg tcttcgaagt tgagcaccag
     1861 cacttcgccg ccgttggccc agacttcttt actcccggcg atctcttctg gtttatagtc
     1921 gccgcccttc accagtaaat ctggcaggat cccggcgatc aagcgctgcg gcgtgtcttc
     1981 ttcaaacgac accacccagt cgaccgcttc cagtgcgccc agcacaatca tacgctgttc
     2041 gagcgggtta acggggcggg aatccccttt cagccgtttg gtggaggcat cgctgttcac
     2101 agcgacaatc aagcggtcac ccagtttgcg ggcatttgcc agataggaga cgtgtccggc
     2161 gtgcaggatg tcaaagacgc cattggtcat caccactttt tcaccgcgtt tacgcgctgc
     2221 cgctacggcc agcttcagtt cctcttcggt catcacgcca aagccggtat ctgcgcgccc
     2281 acgcactgca ttttccagct cgatcggcga aaccgtggag gttcccagtt tgccgaccac
     2341 cacgccagcc gccgcattgg caaagaagca ggcttcttcc agcgaattac ccgctgccag
     2401 cgttgccgcc aggacgccaa tcaccgtgtc gcctgcaccg gtaacgtcat acacttcctg
     2461 cgcctgggtt ggcatatgca gcggcgcttt acccggttgc agcagcgaca taccctgttc
     2521 ggaacgggtc actaacagag ccgagagttc gtaatcggca atcagtttca tgccgcgctc
     2581 aacaatctct tcttcggtct tacatttacc gacaacagct tcaaattccg agagattcgg
     2641 tgtcagcagc gtagcgccgc ggtagcgctc aaaatcggta ccttttggat caatcagcac
     2701 cggaacgccc gctttacgcg ccagttggat catctgctgt acgcttgcca gcgcaccttt
     2761 ggcgtagtca gaaagcacca gcgcgccaat cgaactcagc gcctgattaa tccgctcgtg
     2821 cagcggctgc ggatcaacac cttcgaaacc ttcttcaaaa tccagacgga tcagctgttg
     2881 gttgcgggaa agtacccgta atttggtgat ggtcggatgc gtcggtacag aaacgaagtc
     2941 gcatttgacg ttgacgtcgg ccagagattt actcagcgcg cgcgctgcat cgtcaatgcc
     3001 cgtcaacccg accaggcgtg cattagcacc gagagaagcg atattcatcg ccacgttagc
     3061 cgcgccgccc ggacgttctt cgatggtatt cactttaacc acgggcaccg gcgcttccgg
     3121 cgagatacga ctggtggggc cgtaccagta acgatccagc atcacatcac caaccaccat
     3181 cactcctgca cgttcaaact ctggcagcgt tactttcatt cctgtctcct gagagattca
     3241 aaatttgcgc gcgataatac catacttcat tcttccacca gccacttctg ccagcttgcc
     3301 cgcaccagat cacgctctgc ggtgaagcaa tcctccgaca catggcccgg caattcctgt
     3361 aatgccagat gatgaagttc atcgcgcagc gtcgtgtaag cacgggtcag cgccatcgct
     3421 tcctgctctt ccataatgtc gttttgcgcc agcagctcca gaatacgcac gttgtctgac
     3481 cagcgcgtta acttcggttt ttcatgagcg tagcgcaaca ccagatattg ggtaataaat
     3541 tcgatatcgg taattccgcc ttcatcggct ttgatatcaa agcgattacg atgtttattg
     3601 ccgagatgag cgcgcatttt ctcgcgcatt tcccgcactt ccgtttgcag agttttacct
     3661 tctcgcggca gtgtcataat ctcacgacgc accgcgtcaa agtgcgcagt gagctgcgga
     3721 tcgccgtaca ctacacgcgc acgcaccagc gcctgatgtt cccacgtcca ggcctcgttt
     3781 ttctgataat cggcaaatgc ttctgcggat gtcaccagca ttcccgccgc cccggacgga
     3841 cgcagtcgag catccacttc atacaaaatg ccggaagagg tacgcgtact gaacagatgc
     3901 ataatgcgtt gcgccagacg cagataaaac tgccgcccgt cgatttcccg ctcgccgtca
     3961 gtcatcgcat ccattgggca atcatggagg aagataaggt caagatcgga actgtagcct
     4021 aactcccagc cgcccagctt gccgtagccg accaccgcaa aaccgcgccc ttcacgttcg
     4081 ttgaggtgat ttggcttacc gtagcgggcc accatttgac cccacgcctg ctgaacgacg
     4141 gcatctatca tagcttctgc cagccaggtt aagtgatcgc tcactttcat taccggtagc
     4201 gtaccggcga tatccgctgc cgcaatgcgt aacagctgcg cctgtttgaa ctgacgcagc
     4261 gcctcaagct gttgttcttc gtcatcttcc ggcacgcgca gcaaatactg gcgcagttca
     4321 tcacggtagg catcggtcgc cgtcggctgg taaagggtat ttggatcgag caattcatcc
     4381 agcaataatg gataacgcgc tagccggctg gcaatcatcg gcgatgcggc acacagagaa
     4441 atcaaatgtt taagcgccgc agggaattca ctgagcaatt ctaaataggt tgtgcgggta
     4501 acaatcccca ccagcaaggc ggtaatgcgc gacagcgtga cagcagcgtc ttcacgcgca
     4561 cagacatcac ttagcagatg tggcatcaga tggtcgagca cctgacgtcc tcgcggcccg
     4621 atagtacgct tatccagctc tttgcggaaa tcagcaatca gcgttaggac ctgtttgcga
     4681 tcatcctcgc taagatgcgc cagcactggc gtagtgtcat cttcctgcaa cgcatcctgc
     4741 cacagctcac gccactgttc cgacagcgac tcttcctgag tttcactttc gtcgtcgcca
     4801 atcaattcat taaacacccg gcgcacattg gtcatatgtg cggtcagcgc cccggtcagt
     4861 tgcggccagt cagcaaagtc catcgcccac gccagccgcg cacggttaag ctcatcagaa
     4921 ggaagcgttt gggtttgttc gtcgttaatg ctttgcagca ggttttccag acgccgcagg
     4981 aacagatatg ccactcgcaa ttgttcagca tcgttttcag aaagcagatg cagcgcggca
     5041 atggcgctga gcgttggcag taaagagcgc gactgcagcg acggttcgcg cccaccgcga
     5101 atgagctgga acacctgaac gataaattca atttcgcgaa tgccgcctgc gccgagtttg
     5161 atattgtcgg tcaaaccacg tcgacgcact tcacgggcaa tcatcccttt catgttgcgc
     5221 agcgactgaa tcacgctgaa atcgatgtaa cgacggaaaa caaacgggcg cagcatcgca
     5281 cgcaactcgt tagcatagac gccttcgcta tcgcccataa tccgcgcttt gaccatcgcg
     5341 taacgctccc agtcgcgtcc ctgctcctgg taataatcct ccaacgcggc aaagctcagc
     5401 accagcgggc cactttcgcc aaacggacgc agtcgcatat ccacgcgata gacgaagcca
     5461 tcctgcgttg gttgatccag cactttaatc agccgctgcc ccatgcgggt aaaaaactgc
     5521 gcgttatcca gttcccggcg tccgccctgc gtacaaccat gttccggcca ggcaaaaatc
     5581 agatcgatat cagaggagaa attcagttcc ccgccgccca gcttacccat gcctaagatc
     5641 agcagcggtt gcgcttcgcc ctgcgcattg cacggcgttc cccactcgcg gcagcaggcg
     5701 tcatacagcc agtcacgcgc cgcaacaatc agcgtctccg ccagataact gagctgctgc
     5761 aatatgctct cttcagtaac cagtgccagc gtttgcgccc atgcgatgcg caccataatg
     5821 cgccgccgga atagccgcag ctcgcgcatt aacccggctt cgtcgctcac attactgagc
     5881 gcctcctgca accacacgct gtaatgttgc cattcgtcag cctgtggcgg ttggctttcc
     5941 agttccgtca gccactctgg atgcgcaatc acgctgtcct gcacaaaatc actgaaagta
     6001 agtactgact tcgcctgtgc actaagtgat tcctcggcta aaagctctgg cagccgctca
     6061 acaacggtct gccagtactg ctgtaacggt gaagagagcg gcttcataag cgattttatc
     6121 cttgcctgat taacgttttc cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt
     6181 acggaaatgt tcaatttcaa tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca
     6241 gttctccagc caggcttgcg cgacgacagg atcatagtaa cccgccagca acagtattga
     6301 gtcaatatca cgcatcaggc gtggcaactg gtcacggtag cgatcgccta acggctggca
     6361 gaaaacgctt ttcagttcag cggcatggcg ggaaagatgg atatcggcaa agcgtttgaa
     6421 ggagtcgcta attttgccct gcgctttggc atctaaaaac ggctgccatg ctttgcttac
     6481 cagccattcg gtcaacgcca gcttcgccat tgccgtttcg gtagagtaga ccgccgtcac
     6541 ggcagaaacc gcagaagcga tggtcgcttc gcattgagtc agcaaatcac gtaagtgagt
     6601 gctcgcttta cgcggcacaa tgctaccgaa cagcatcagt gtatgacgga ctaggctaat
     6661 ggctgccagc acctgctctt tcgccgcatc gttgccgcgt acccacagtt cttcatgata
     6721 ctgccattgc gctaacacca gctcgagcgc cgcttccagc ccctgttcca catcggcttt
     6781 tgccgcaaca tgcaaaatgg tggtcggttt gatttcacgc gccggattgc cctgcgccag
     6841 atgatagcca cgcgccgctt tgctcaggct gccctggcgt aatccggttt gcgataccag
     6901 ttggttcgcc agtttcagca ccgcgcgcgt gtcgccgcta agcagctcca gttccagctc
     6961 gcagataggc tcagcaaatt cacccgcttt cacttccccc tggtcgagag cgatttcaat
     7021 tcggctgcca tcgatctcta ctagccattt ttcgcgataa aaatcggtgc taaacagcgg
     7081 ctgcacgcgg gaagcgagat cggcaggcaa ttcgccgttc ggccagattt ccgtcggtaa
     7141 ctgcgccagg tcgagcgtcg gttcgctcaa cgccacgtta tattccgggc gctggtgtaa
     7201 gccgcctgtc actcttcctg caactttcat ggtcatctca tagcgaccgt tttcgccacg
     7261 aatacgtaag cccatatcgt gcctacgcag ccagttatcc ggcgtttcgt agtaaatatt
     7321 cagcaactgc acggggtcat ggtgctcgcc gcccagcgta ttgagatggt cacgcaacgc
     7381 ctcaacggca ctgtgattaa caataaactt taattcgatt tcctgagcca tggcctttac
     7441 ttatcggtta tgtcacataa gtgacgatga acggcgaact taatgcgatc ttttttgtca
     7501 gtagatagta ttttgcgcca aattgccatg caacgggcaa tttgacgggc gtaaaagttt
     7561 gaagcagtgg caagacctga cacagaggat tccgattgat gtcgaatgct ttgcgtagcg
     7621 acactgatgc cactactatc gttccacttt ccatgacaat aacgacagcc tgatgccaaa
     7681 attacgcctg atcggattaa ctttactcgc acttagcgcg actgccgtct cacacgctga
     7741 agaaacgcgc tatgtttctg acgaactgaa tacctgggtc cgtagcggtc cgggagatca
     7801 ttatcgcctc gtgggcacgg ttaacgccgg cgaggaagtg accttattac aaactgacgc
     7861 caacaccaat tatgcccagg tgaaagacag ctctggccgt accgcctgga tcccgttgaa
     7921 acaacttagc actgagccaa gcctgcgctc ccgtgtgcca gatctggaaa atcaggtcaa
     7981 aaccctgacc gataaactca ccaatatcga taacacctgg aatcagcgca ccgcagaaat
     8041 gcagcaaaaa gtggcgcaga gcgacagcgt gatcaacggg ttaaaagaag aaaatcagaa
     8101 actgaaaaac gagctgattg tcgcgcagaa aaaggtcgat gccgccagcg tacagctgga
     8161 tgacaaacag cgcaccatca tcatgcaatg gtttatgtat ggcggtggcg tgctggggct
     8221 tggcttgctg ctcggtctgg tactgccgca cctgatccca agccgcaaac gcaaagatcg
     8281 ctggatgaac taaatcgcct tcttcgttgc actgtcttat tattaggaat tgtgtatttg
     8341 acgaagggat gatggcgtga agatttatct ggtcggtggt gctgttcggg atgcattgtt
     8401 agggctatcg gtcaaagaca gagattgggt ggtggtcggc agtacgccac aggagatgct
     8461 cgacgcgggc taccagcagg taggccgcga ttttcctgtg tttctgcatc cgcaaacgca
     8521 tgaagagtat gcgctggcac gtaccgaacg gaaatccggt tccggttaca ccggttttac
     8581 ttgctatgcc gcaccggatg tcacgctgga agatgatctt aagcgtcgcg atctgaccat
     8641 taatgcgctg gcccaggacg ataacggcga gattatcgac ccgtacaacg gtctgggcga
     8701 tctgcaaaat cgtctgttgc gccatgtttc ccccgctttt ggcgaagatc cgttacgcgt
     8761 actgcgcgtg gcgcgttttg ctgcgcgtta tgcccacctc ggttttcgta ttgccgatga
     8821 aactctgacg ctgatgcgcg agatgaccca tgcgggtgaa ctggaacacc tgacgcctga
     8881 acgggtatgg aaagagacgg aaagcgccct caccacccgc aatccccagg tgttcttcca
     8941 ggtactgcgc gattgcggcg cacttcgcgt tttattcccg gaaattgacg cactgtttgg
     9001 cgttccggca cctgccaggt ggcatccgga aatcgacacg ggtattcata ccttaatgac
     9061 gctctcaatg gcggcgatgc tgagtccgca ggttgatgtc cgtttcgcaa ctttatgcca
     9121 cgatctcggt aaaggactga cgccgccaga actttggccg cgtcatcatg ggcatggccc
     9181 ggcgggtgtt aagttagtgg aacaactatg ccagcgtctg cgcgtgccaa atgaaattcg
     9241 cgatttagcc agactggtgg ctgagtttca cgatctcatc cacaccttcc caatgctgaa
     9301 cccaaaaacc atcgtcaaat tatttgattc catcgatgcc tggcgtaaac cgcagcgtgt
     9361 cgagcaactg gcgctgacca gcgaggctga cgtgcgcggc agaaccggtt ttgaatcagc
     9421 ggactacccg caaggccgtt ggttgcgcga agcctgggaa gtggcgcagt cagtgccgac
     9481 aaaagcggtc gttgaagcgg gatttaaagg cgtggagatt cgcgaggagc tgacccgacg
     9541 gcggattgcg gcggtagcca gctggaagga acaacgttgc ccaaagcctg actgagtata
     9601 aaactaaacg ttagcacgaa ccgtcccctc gccccttcgg ggagagggtt agggtgaggg
     9661 gaacagacca gcaccggtgc gaacaatcac ccccaccccg gccctctccc tacagagcga
     9721 gggagaaaac ccggcctgct cccgaacctt ttctcaatca aaagaacacg acatacaccg
     9781 cagccgccac aataaagcga taaatggcga acggggtaaa cgaaatgcgc ttaatcaatt
     9841 gcaggaaggt tttaatcgct atcagcgcca ccacaaaagc ggtgataaac ccaacggcaa
     9901 acatcgggat atcgccggtt gtcaggaagc cccagctttt gtagagatcg agcgccgttg
     9961 cgcccatcat catcggtacc gccagcagga acgagaactc ggaagcagcg taacggctca
    10021 cgcccatcag catcccacct gaaatggtcg ccccggaacg ggagaaaccc ggccacagcg
    10081 ccagacactg gaaacagcca atcataaacg cctgacgata ggtcatatca tcaagacccg
    10141 gcgcacgcgg ctctttcggc ttcaggcatt cggcggcaat cagcaacaaa ccgccaacga
    10201 ccagcgcata catcacattt atcgggttaa acaatgactt aatcgtgtcg tggaataata
    10261 gccccagtac caccgccgga atcatcccca gcaaaatgtg gatcagcgtt aaacgacctt
    10321 tgctttcacc ttcgtgctgc aacgggcggc caaagtggat gccaatcagg ccaaacagac
    10381 gccgccagaa catcactact accgccagaa ttgatcctaa ctggatcaca acttcaaagg
    10441 ttttcgccgt gtcgccctca aaccccaaca agtgaccgac aataatcata tggcccgtgc
    10501 tggataccgg cagaaattct gtcaatcctt cgaccacacc caatattgcc gctatcagca
    10561 gcgagtgcat atcgctcatc aataaacccc taaattatta aaatgtaccg cttgtccgaa
    10621 ctactgcagt atgaccaggt tataaccgtt tggtttaaca gctgtaaaat taattatttt
    10681 ctttcagatt attgccacgc tcaatgatta cgccaacatt cgccgcccgc gccactgcgc
    10741 ctggcttgct gagtttgata cgcacccacg gcgagttgaa gcgtgctaac agcagctccg
    10801 ccacctcttc agccacgcgt tccaccagcg caaaacgcgc cccctcgacg tggctgacca
    10861 ccgtttctgc aatgtcagcg taactgaggc aatccgccac atcatcactt ttcgccgctt
    10921 tacggttatc ccatgccatt tcgatatcga acactaactt ctgttcgatg gtctgttccc
    10981 agtcgtaaac accaatagtg gtgattaccg aaagttgctc tataaataca atatccatca
    11041 cgtcctgcct gctttttggc taaccggata ccacttccgg cgaaatgtgc gtattatcca
    11101 cagattcatc gttgaacacg aattttcaaa acggaacagc ttatgagtgc aatcgcgctt
    11161 ggaatgatcc tcatcgcgta cctctgcggc tccatttcca gtgccattct ggtttgccgc
    11221 ttgtgtgggc tgcccgatcc gcgaaccagc ggctccggca atccaggcgc aaccaatgtg
    11281 ttacgtatcg gtggcaaggg agcagccgta gcagtactga ttttcgacgt tctgaaagga
    11341 atgttgcctg tctggggcgc gtatgaatta ggtgtcagcc ccttctggct aggcttaatt
    11401 gccatcgccg cctgtcttgg acacatctgg cccgttttct tcggttttaa aggaggaaaa
    11461 ggcgttgcta ccgcttttgg agcaatcgca ccaattggct gggatctcac cggagtaatg
    11521 gcgggaacct ggttactgac cgtgctattg agcggatact cgtcgctggg agcgattgtc
    11581 agtgcactga ttgctccgtt ttatgtctgg tggtttaagc cacaattcac cttcccggtt
    11641 tcgatgctct cttgcctgat cctgctgcgt catcatgaca acatccagcg tctgtggcgt
    11701 cgtcaggaga caaaaatctg gacgaaattc aaaagaaagc gcgaaaagga tcccgagtga
    11761 tttctggtgg atctacatga cctgatagcc ttcatcgggc ttgcccagcc gttgctggca
    11821 ccatgccgcc agaaattcca cgcagacacg taatttcatg ctgcgataga gcggctcccg
    11881 gtaaacagcc cagatattgg cgctttgtgc atactccggc aatacttgca ccagtttgcc
    11941 actctccaga aacggcaaca catcccactc ggaacgcagc ataatccctt tgccctccag
    12001 cgcccattgc aacactatct cgccgctatt ggaggaaaga tgcccgctaa ccttcaccga
    12061 ttttttctcc tgaccgttcc ccaactccca tattccgtgg gtcatatcgc gctctttggt
    12121 caccagacag tcatgacgac ttaattcctg taatgattgt ggctgcggat atttttgcag
    12181 atactcaggt gctgcgcata atattctttt atttttcgtt aacagatgcg caatataata
    12241 atcaggaatt tcgtcattaa tacgaatatc cagatcaata ttatcctgca ctaaatcaat
    12301 ttgccgatcg aacaattcaa aatgcacctg taactcagga taattgcgca taagttcggt
    12361 aatggctggc gcaatatggc tgcgcccaaa accaaaactg caaccaatac gaatcatcct
    12421 ctccgggcgc gttttgattt gcgtgacgtc atcgaccagt cgctgatatt gggtgaggat
    12481 ttccagcgca tgttcgtaac aacgttggcc actttcagtc agcgccacgc cgcgagcgga
    12541 gcggttcagc agcgttgttg ccagcgtatt ttcaaggatt tggatccgct tggtgacaaa
    12601 tgcaggcgtt tgcccaagcg tcgctgctgc agcactaaaa ctcccgctgt ggacgatttc
    12661 taccagtacc tgcaagtctt tggctaaagg ccagctattc agcatcgtta attatccgca
    12721 gttgtgataa gcgcagtgta tttcgcaaaa cattgccatt ccctgctctg gcgcaatatt
    12781 cattcttatt ttcgcgttga ttcacgaact gttaattaca tattcacggt ggcaaaaaat
    12841 ataaaaccac atttttagtg gtagtttgtg gcggtgaatt tttccagaca aatacaaaaa
    12901 ctggagttgc catgatgagc gaaagtaata agcaacaggc agtgaataag ttgacagaga
    12961 ttgtcgctaa ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg gtggataaac
    13021 taaaacagct aaaggatgcc gaaacgtcgt cgatggggaa aattatctac catacgatgt
    13081 tcgacaacat gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac accggggaga
    13141 ttatgttctt cgttaaagtc ggttcccgct tcccactgct tggcgagctg caaagcatac
    13201 tcaaacaagc cgtggaagag gcaaccgtca aagcgccact acgtcacaat gcggtagaaa
    13261 tttttgacga agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg gtcacctggg
    13321 acatcatccc cgacaatgac gatgcggaaa tcgaagttta catggcaggc ggcggctgca
    13381 cgctacctgg ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc gtggtgaaat
    13441 tcgtcttcga aaatatctcc accctcgccg taaacgcctg tccaccggta ctggtgggcg
    13501 tgggcatcgc cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc attttgcgcc
    13561 cgattggcag ccgccatccc aatccaaaag cggcagaact ggagctacgc ctggaagaag
    13621 gactcaaccg tctggggatt ggtccacaag ggctgaccgg caacagttca gtgatgggcg
    13681 catcgtcgcg gcacgctgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc
    13741 cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata
    13801 ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgc gacgtttgtc
    13861 accgccgttt gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt
    13921 tccacgctgg ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggtc tccgtcggcc
    13981 cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tattgagcag accggcgtaa
    14041 aactggtggt tggcaaaggt ggtatggggc cgctgaccga agaaggctgc cagaaattca
    14101 aggcgctaca tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc caggtagaag
    14161 agattgaaga agtgcactgg acagaactcg ggatgccgga gtcactgtgg gtctgccggg
    14221 tcaaagagtt cggcccgctg attgtctcta ttgataccca cggcaacaac ctgatagccg
    14281 aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg
    14341 tccattacat caaataaccc tcccggagag gctcacccct ctcctttttc gcaggcataa
    14401 cacgatgaaa ccttccactg aatggtggcg atatcttgcg ccgctggcgg tcatcgccat
    14461 tattgctcta cttccggttc ccgcagggct ggagaaccat acctggctct actttgccgt
    14521 ttttactggc gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg tggtggcgat
    14581 ggtgggtatc tccatcatcg ccatactctc tccctggctg ctgttcagcc ccgaacagct
    14641 cgcccagcca ggctttaagt tcaccgccaa atccctctcc tgggcagttt ctggtttttc
    14701 taattcggtt atctggctca ttttcgccgc ctttatgttt ggcacaggct atgaaaaaac
    14761 cgggcttgga cgccgcattg cgctgattct ggtgaaaaag atggggcatc gcacgctgtt
    14821 tctcggctat gcggtgatgt tctccgagct gatcctggca cctgtaacac cgtccaactc
    14881 ggcgcgtggt gcgggtatta tctatcccat catccgtaac ctgccaccgc tctatcaatc
    14941 acaaccaaac gacagcagtt cgcgcagcat tggctcgtac atcatgtgga tggggattgt
    15001 tgccgactgc gtgaccagcg ccattttcct gacggcgatg gcacctaact tgctgttaat
    15061 tggactgatg aaaagcgcat ctcacgccac gctgagttgg ggcgactggt tcctcgggat
    15121 gttgccgctc agcattttac tggttctgct ggttccctgg ctggcttacg tgctgtaccc
    15181 gccggtactg aagtctggtg atcaggtgcc gcgctgggca gagacggaac tgcaggcaat
    15241 gggcccgctc tgttcgcgtg aaaaacggat gctggggctg atggtaggcg cgctggtgct
    15301 gtggattttc ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg tggtggcgct
    15361 gatgctgctt ctgctcatta tcagttggga cgacattgtc agtaataaag cggcatggaa
    15421 cgttttcttc tggctggcct cgcttatcac cctcgcgacc ggactcaaca ataccggttt
    15481 tattagctgg tttggcaaac tgttagcagg cagcttaagt ggttattcgc cgacgatggt
    15541 gatggtgacg ttgattgtgg tgttttatct actgcgctac tttttcgcca gcgccacggc
    15601 gtatacctcc gccctcgcac cgatgatgat cgccgccgcg ctggcgatgc cggaaatccc
    15661 gctgccggtg ttctgcctga tggttggcgc ggcaattggt ctggggagca ttcttacacc
    15721 atacgccacc ggacccagtc cgatttacta cggtagtggt tatctgccaa cggcggatta
    15781 ctggcgactg ggggcgattt ttgggctgat attcctcgtg ttgctggtga ttaccggctt
    15841 actgtggatg cccgtggtgt tgctttaagt gaggacggtg ccggaaattg ctctccggca
    15901 cccaatagtt ttacgcagcc ggtaactccg ccagcggcca gcgcggacgc acgctaacgc
    15961 cgagatccgc cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg atcatcgcgc
    16021 cgttatcagt acaaaattcc ggacgcgcgt agaacacttc gccgcggcgt tttttcatca
    16081 tttcagccag cttcgcccgt aacgtgcggt tagcactcac gccgcccgcc atgaccagtc
    16141 gcttaaagcc cgtctgatcc aacgctcgct tacacttaat catcaacgta tcgaccaccg
    16201 catcttcaaa ggcgcgggcg atatcagcac gcgtctggtc gtcggtgccg ttgtcacgaa
    16261 tcgtgttcgc cgcaaaggtt ttcagaccag aaaagctgaa atccagcccc ggacggtcgg
    16321 tcatcggacg cgggaaaaca aagcgcccgg cagtaccctg agccgccatt ttcgacagta
    16381 acggtccgcc aggataatcc agccccagca gcttcgcggt tttatcaaac gcttcaccgg
    16441 cggcatcatc gatagactcg ccgagcagct cgtactgacc aatgccagtc acgctgatta
    16501 actgcgtatg accgccggac accagcagcg cgacaaacgg aaattccggc gggttatctt
    16561 ccagcatcgg cgctaacaga tgcccttcca tatggtgtac cgggattgcc ggaacgttcc
    16621 aggcaaacgc cagagaacgc cccacggttg cgccaaccag cagtgcgccg actaatccag
    16681 ggcctgcggt ataggccaca gcatcaatgt cttttgccgt taaaccagac tcctttagcg
    16741 ccgcctggat caacggtacg gttttacgca catgatcgcg ggaggccagt tcaggcacga
    16801 cgccgccgta gtcagcgtgc aatttcacct gactatacaa ttggttggct aacaaacctt
    16861 tttcatcgtc gtaaatggcg atgccggttt catcgcagga agtttcaata cccagtacac
    16921 gcatgacttg ttttacctcg ctttattacc gcgcagtgta ggaccaatgc gggttgatgt
    16981 aaaactttgt tcgcccctgg agaaagcctc gtgtatactc ctcaccctta taaaagtccc
    17041 tttcaaaaaa ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca
    17101 ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca
    17161 catgccggta attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa
    17221 gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa
    17281 accgactacc gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact
    17341 ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg
    17401 atcagaccga gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg
    17461 tttatgaatt gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat
    17521 gatctgctgg cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag
    17581 cagggcaaga atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc
    17641 gttaacggtg agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc
    17701 gacttcctga tgaactacga caagctcgag ttcgtcgaaa cggtcgaaga gctggcagca
    17761 atgcacaatc ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgagcgccat
    17821 cagaggcaaa cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta
    17881 caacaacctg ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag
    17941 gttatcgctc gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg
    18001 tttggcggca atccagaaaa tcgccagtca ttgattgatg cggggatgtt ggtcactaac
    18061 gatcagggac gcagttacga tcgtttccgc gaacgggtga tgttccccat tcgcgataaa
    18121 cgcggtcggg tgattggttt tggcgggcgc gtgctgggca acgatacccc caaatacctg
    18181 aactcgccgg aaacagacat tttccataaa ggccgccagc tttacggtct ttatgaagcg
    18241 cagcaggata acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg
    18301 gcgctggcgc aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc
    18361 gatcacatac aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac
    18421 cgtgcaggcc gcgatgccgc atggcgagcg ctggaaacgg cgctgcctta catgacagac
    18481 ggccgtcagc tacgctttat gtttttgccg gatggcgaag accctgacac gctggtacga
    18541 aaagaaggta aagaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt
    18601 ctgtttaaca gtctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg
    18661 agtacgctgg cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt
    18721 cgtcaggaat taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg
    18781 ccaaaagcgg cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg
    18841 cgtatactta tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt
    18901 gagaatctgg atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact
    18961 tgtctctccc agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat
    19021 aatgctgcca cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct
    19081 gagcaaacct tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag
    19141 gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc
    19201 tggacattaa accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga
    19261 tcgggaagcc cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt
    19321 aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg
    19381 tcaatcgccc aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc
    19441 cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg
    19501 ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca
    19561 tccaaatgat caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc
    19621 tgatgctggc tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt
    19681 ccagcgtgga atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa
    19741 tgggcaccgt tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag
    19801 acgggatcaa ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc
    19861 tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct
    19921 ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc
    19981 tttcccagga agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca
    20041 gcgccgatga tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactac
    20101 gcgctcagta cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg
    20161 ctcaggaaga gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc
    20221 agtttgacta cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac
    20281 gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc
    20341 tgtttaccgg caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc
    20401 cgtggtcgga aaaactgcac gatgtctctg aagaagtgca tcgcgccctg caaaaactgc
    20461 agcagattga agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta
    20521 tgtccatcgg tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac
    20581 gtctggttat ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga
    20641 ttcaggaagg taacatcggc ctgatgaaag cggttgataa attcgaatac cgccgtggtt
    20701 acaagttctc cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg
    20761 atcaggcgcg caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc
    20821 gtatttctcg ccagatgctg caagagatgg gccgtgaacc gacgccggaa gaactggctg
    20881 aacgtatgct gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa
    20941 tctccatgga aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg
    21001 ataccaccct cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc
    21061 acgacgtgct ggctggcctg accgcgcgtg aagcaaaagt tctgcgtatg cgtttcggta
    21121 tcgatatgaa caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg
    21181 aacgtatccg tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg
    21241 aagtgttgcg tagcttcctg gacgattaat cggtaggccg gatcaggcgt tacgccgcac
    21301 ccggcactag tctctctgca caaacgccac cttt
//
LOCUS       DBKEKI010000059        21329 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.059, whole genome shotgun sequence.
ACCESSION   DBKEKI010000059 DBKEKI010000000
VERSION     DBKEKI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.059"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..865
                     /locus_tag="ACUQPX_002247"
     CDS             <1..865
                     /locus_tag="ACUQPX_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HHV9056950.1"
                     /translation="FILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKELTHK
                     LESIFNNTQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTV
                     RIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPAN
                     PSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLF
                     SDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSGGGG
                     SSGGGGASGRW"
     gene            complement(925..2223)
                     /gene="eno"
                     /locus_tag="ACUQPX_002248"
     CDS             complement(925..2223)
                     /gene="eno"
                     /locus_tag="ACUQPX_002248"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HHV9056951.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2311..3948)
                     /gene="pyrG"
                     /locus_tag="ACUQPX_002249"
     CDS             complement(2311..3948)
                     /gene="pyrG"
                     /locus_tag="ACUQPX_002249"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HHV9056952.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4176..4967)
                     /gene="mazG"
                     /locus_tag="ACUQPX_002250"
                     /pseudo
     CDS             complement(4176..4967)
                     /gene="mazG"
                     /locus_tag="ACUQPX_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
     gene            complement(5063..7297)
                     /gene="relA"
                     /locus_tag="ACUQPX_002251"
     CDS             complement(5063..7297)
                     /gene="relA"
                     /locus_tag="ACUQPX_002251"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HHV9056953.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGIISQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7345..8646)
                     /gene="rlmD"
                     /locus_tag="ACUQPX_002252"
     CDS             complement(7345..8646)
                     /gene="rlmD"
                     /locus_tag="ACUQPX_002252"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HHV9056954.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLGALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLQEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8703..11459
                     /gene="barA"
                     /locus_tag="ACUQPX_002253"
     CDS             8703..11459
                     /gene="barA"
                     /locus_tag="ACUQPX_002253"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HHV9056955.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPPSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11691..13031)
                     /gene="gudD"
                     /locus_tag="ACUQPX_002254"
     CDS             complement(11691..13031)
                     /gene="gudD"
                     /locus_tag="ACUQPX_002254"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HHV9056956.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13052..14392)
                     /locus_tag="ACUQPX_002255"
     CDS             complement(13052..14392)
                     /locus_tag="ACUQPX_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HHV9056957.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14394..15746)
                     /gene="gudP"
                     /locus_tag="ACUQPX_002256"
     CDS             complement(14394..15746)
                     /gene="gudP"
                     /locus_tag="ACUQPX_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HHV9056958.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKSVAGQ
                     "
     gene            complement(16180..16629)
                     /locus_tag="ACUQPX_002257"
     CDS             complement(16180..16629)
                     /locus_tag="ACUQPX_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HHV9056959.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16647..17429)
                     /gene="truC"
                     /locus_tag="ACUQPX_002258"
     CDS             complement(16647..17429)
                     /gene="truC"
                     /locus_tag="ACUQPX_002258"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HHV9056960.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17429..17758)
                     /locus_tag="ACUQPX_002259"
     CDS             complement(17429..17758)
                     /locus_tag="ACUQPX_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HHV9056961.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18380..18925)
                     /gene="syd"
                     /locus_tag="ACUQPX_002260"
     CDS             complement(18380..18925)
                     /gene="syd"
                     /locus_tag="ACUQPX_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HHV9056962.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18993..19841
                     /gene="queF"
                     /locus_tag="ACUQPX_002261"
     CDS             18993..19841
                     /gene="queF"
                     /locus_tag="ACUQPX_002261"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HHV9056963.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19953..21317
                     /gene="ppnN"
                     /locus_tag="ACUQPX_002262"
     CDS             19953..21317
                     /gene="ppnN"
                     /locus_tag="ACUQPX_002262"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HHV9056964.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPT
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5235 a         5689 c         5097 g         5308 t
ORIGIN      
        1 tttcatacta atgttcactt tcgtatgttc ctttgttgca gcccaaccaa ctattgtccc
       61 acaattacag caacaagtta cgggtcttac gagtagctta aactcacaag aaaagaaaga
      121 actgacgcac aagttagaat ctattttcaa taatacccaa gtgcaaattg ctgtattaat
      181 tgtttccaca accaaagatg agacaattga acaatatgct acaagagttt ttgacaattg
      241 gcgtttagga gatgccaaac gtaatgatgg gatactgatc gttgttgcct ggtcggatcg
      301 cactgtccgc atccaggtag gttatgggct ggaagaaaag gtaacagatg ctctggctgg
      361 agatatcatc cgtagcaaca tgatacccgc atttaaacaa caaaaattag ctaagggatt
      421 agagttagct ataatcgctt tgaataacca actcacttca caacaccaat atccggctaa
      481 tccttcagaa agtgaatcaa cgtcttccac tgatcattat tactttgcta ttttttgggt
      541 atttgcagtg atgttcttcc ctttctggtt ttttcatcaa ggcagtaatt tttgtcgcgc
      601 atgtaaaagt ggagtctgta tttcagcaat ccatctttta gatttattcc tgttctccga
      661 taaaactttt tccattgctg tattttcctt cttttttact ttcaccatat ttatggtctt
      721 tacctgttta tgtgtacttc agaaaagagc atctggtaga agttatcatt cagacaacag
      781 cggttccgct ggaggctcaa gtggcggcgg atttagcggc gggggcggtt cttctggcgg
      841 tggcggcgca tccggccgct ggtaactcca ccataaaaaa tgccagcccg aaggctggca
      901 ttatcaaatc agataaagtc agacttatgc ctggcctttg atctctttac gaccgttgta
      961 cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt tagcaacacg
     1021 gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa cagccagatc
     1081 agcgatggta gcatcttcag tttcaccaga acggtgagag ataactgcag tgtagccagc
     1141 atctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct ggttgaattt
     1201 gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct tggtgttggt
     1261 tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc agaactttgg tctggtatgc
     1321 gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata gaaacgatcg ggtactgttt
     1381 ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aacgctttgt tgccttcgcc
     1441 agccagaacg tatttaccat ctttgtagaa ttcagaagct gcgcagtcca tcgccagagt
     1501 gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa cagccagagc
     1561 ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaacag cagtgttcat
     1621 gcctttcgct ttcagaactt ttgccaggtg atggaaaact tcagaaccca tgcggatggc
     1681 ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat caacgttgtt
     1741 gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatgg agtatttgcc
     1801 cggagtgccg ttcagttcag cgatgtgctc gtacagcggc atacctttag cagctgcagc
     1861 agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt tggatttgtt
     1921 ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt tagcatcttt
     1981 gccaatcagc gcctgagcga tcgggccgtt taccgcagca acagctttgg ttacgccttt
     2041 acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg aaccagtaga
     2101 agcacctgac ggagcagctg ccataccgac gaaaccaccc tccagatgta cttcggcttc
     2161 aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta cgattttgga
     2221 cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa cgcgtaccca
     2281 gggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgtttct ggaactcgct
     2341 ggcggctttc acaaagcctg caaacagcgg gtgaccatca cgtggagtag aagtaaactc
     2401 cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga tctcgaccaa
     2461 ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct gtttcaacag
     2521 catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg cattgtatag
     2581 ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac gcatggtacc
     2641 gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc gccactcggt
     2701 aatcagcgcc acaaccgggt acttacagtc tggcacaaat tctgtagagt tggcgttctc
     2761 catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca ggcaaatgcc
     2821 cagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc cttctacgcc
     2881 acgatagccg aaaccgccag gtacgaggat tgcgtccaga cctttaagga tttcaacgcc
     2941 gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac gattcttcag
     3001 cccaccgtgt ttcagtgctt cgatcactga tttataagca tccggcagtt caatgtactt
     3061 gccgaccata ccgatggtga cttcacttac cggattcgct tcttcgaaga taacctgttc
     3121 ccattcggac agatttgctt ccgggcagtt taagctgaat cgtttacaaa tataatcgtc
     3181 cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt ctttcagaga
     3241 aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt cgttcgccgg
     3301 tacagcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg agagcagctc
     3361 ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca tgtacggcac
     3421 cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca tctggcgaat
     3481 agcttcgagg aacggcaagg attcgatatc acctactgta ccgccgattt ctaccagtac
     3541 tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag tgatgtgcgg
     3601 aataacctgc acggttgcgc cgaggtagtc accgcggcgt tctttacgca gaacgtcaga
     3661 gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgaa tgaaacgctc
     3721 gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca cttccccgtg
     3781 ttggattggg ctcatagtac ctggatcgac gttgatgtaa ggatccagtt tcatgatggt
     3841 cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa tgcctttacc
     3901 cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc tgaacctgag
     3961 aagttaggtt gaaaagacga tggaataacc aagacgggaa agcagtatac ccgaacatga
     4021 cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga aatcaggagt
     4081 gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt gtcatttaaa
     4141 aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc ctgccgtttt
     4201 acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc cagtccacgc
     4261 gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt cgctttttgc
     4321 aatgcgattt ctgctttcgt ccctaaatgg cgagccagat taaccgtggc aaacagcagg
     4381 tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc ctcgtacatc
     4441 acctcgtcga tctcttcgta gactttatca accaccgggc caagcgtcgt ccagtcgaag
     4501 ccaacgttgg cgcaacgttt ctggattttt tgcgcccgca ttaaagccgg taaactacga
     4561 ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt tttgatttgc
     4621 tcccaacggg caagcacttc actactgttt tcggcagaac tatcagcaaa aacatgcgga
     4681 tggcgacgct ctaatttatc gctaatagca gcgcaaatat cattaaagtc aaagcgccct
     4741 tcttcctgag ccatttgcgc gtaaaacacc acctagaata gcagatcgcc cagttcaccg
     4801 cgaagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt ttcttcaagg
     4861 gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc gttttccgga
     4921 tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt gattcattga attgtcctga
     4981 aaattgcggg tctgtcaggt gtaatacctt cgagtaaaag atatcgctgg atacggctga
     5041 cgccatatcc agctatgtaa gactaactcc cgtgcaaccg acgcgcgtcg ataacatccg
     5101 gcacctggtt gagtttaccc agcacgcgcc ccagcacttg caggttgtaa atctcaatgg
     5161 tcatgtcgat ggtcgccagt tgctgtttgg tgtcgctacg gctggcaacg ccaagcacgt
     5221 tcaccttctc gttggcgaga atggtcgtga tatcacgtaa caacccacta cgatcattag
     5281 ccaccacgcg gaccaccagc gaatatccag cggagtagct ctcaccccat accgcgtcaa
     5341 caatgcgttc tggcgcatgg gagcgcagtt ccgccagttg ttcgcaatcg gcgcggtgta
     5401 ctgaaatacc gcgtccctgg gtaatgaagc cgacaatctc atctccagga atcggctggc
     5461 agcagcgcgc gatgtggtgc atcaggttac caacaccttc aaccaccaca cgaccgttat
     5521 ctttactgcg gttttgcggt gtgtagcttt tttgctgaag ctgtttcagc gcggcggcgt
     5581 cctgctcttc ggcactcggc ttattaaatt gcgattgcag gaagttcacc atctgattga
     5641 gacggatatc cccgccacca atcgccgcca gcaactcgtc gacatcattg aagttgtaac
     5701 gcggcagcag atgtttttct gcttctttca ggctgatccc cagatgttcc agctcgtcgt
     5761 caaggatttg ccgcccagcc agaatgtttt tgtcacggtc ctgtttacgg aaccaggcgt
     5821 gaattttcga acgcccacgg ctggttgtga cgtaaccgag gtttgggttt aaccagtcac
     5881 ggctggggtt cggctgtttc tgggtgataa tttcaatctg gtcgcccatt tgcagctggt
     5941 aggtgaacgg cacaatgcgc ccgccaattt ttgccccgat gcagcggtgt ccgacatcac
     6001 tgtggatgtg gtaagcgaag tccagcggcg ttgatcccgc aggcaaatca acgacatcac
     6061 ctttcggcgt aaagacatac acccggtcgt caaacacctg actacgtact tcgtcgagca
     6121 tttcgccgga atcagccatc tcttcctgcc acgcaatcag tttacgcagc caggcaatcc
     6181 ggtcttcatg tcccgaacgt gcgccgccag ctgccgcgcc ctctttatat ttccagtgcg
     6241 cagcaacacc caactccgca tcttcatgca tctgtttggt gcggatttgg atctcaacgg
     6301 tttttccacc cggccccaga accacggtat gaatagactg ataaccgttt ggtttcgggt
     6361 tagcgacgta atcgtcaaac tcatccggca ggtggcgata gtgagtgtgc actatcccca
     6421 gtgcggcata acaatcctgt aaacgctcgg cgacaatacg taccgcacgc acatcaaaca
     6481 gctcatcgaa ggcgaggttc tttttctgca ttttgcgcca gatgctgtag atgtgtttcg
     6541 gacgaccata cacttcagct ttaacgcctt cagctttcat ctcagcgcgc agatggccga
     6601 caaactcttc gatatagtgt tcgcggtcga gacggcgttc atgcagcagt tttgcgatgc
     6661 gtttgtattc ggtcgggtgg agataacgga agcagtaatc ttccagctcc catttcagtt
     6721 gcccaatccc aagacggttc gccaacggcg cgtagatatt ggtgcactct tttgccgcca
     6781 gtacacgttc atcttccggc gcatctttta cttcgcgcag atgagcaata cgctccgcca
     6841 gtttgatgac tacgcagcga aaatcatcga ccatcgccaa taacatccgg cgaacgttat
     6901 cgacctgttc ggaggaaaca gaatcagtgt gcgtcgcttt cagctggcgg atcgccgcca
     6961 tatcacgcac gccgtgaata aggttaacaa ccgacttacc gacgctctca cgcagcacat
     7021 cttcgctgac tacgttggca tcagccagag ggaacagcag cgccgcccgc agcgtgtcaa
     7081 tgtccatact taatgtcgag aggatctcca ccatctcaac accacgccac aataacagac
     7141 tggcatccgg atgcccctgc gtctgttgca gacaatacgc ccaggtttcg gctaagcact
     7201 cacacgactt ctggctgata atacccagac ttgcgatcca tttttccgga tcaaattcac
     7261 cagccttatt gatatgtgca cttcttaccg caaccatcgt cctctccttt agggaccaga
     7321 cctgccgaaa tcggcaaatc gtaactattt aacgcgcgag aaaagtacca tcgattccag
     7381 atgtcccgtg tgtgggaaca tatccagcat cgccagtcgc gcaatggtat atcctgcttt
     7441 taataacgct tcgctatccc gagccagcgt tgcagggtta caggatacat aaactatacg
     7501 aataggttcc agttttataa tttgctgcat aacacctgcg gcacctgctc gcgccgggtc
     7561 cagcaacact ttatcgaagc cgtttttcgc ccacggctgc tttgtgacat cttcttgaag
     7621 attttcgtga taaaacgtca cattctgtaa gccattaaga cgcgcattct gctggccttt
     7681 ttccaccagc gccggaacac cttctacacc gaccacactg gcagcttgtg tcgccaatgg
     7741 cagtgtaaag ttgcccatac cgcagaacag atccagtacg cgatcttcag gttgcacatc
     7801 cagccattcc aacgcacgcg ctaccatttt ttggttcaca cccgcattga cctgaataaa
     7861 atcgcgcggg ctaaaagtta agcgcaaccc gtttgagtca taccagggca tctcaccaga
     7921 gacggtttcg agtatctcac tatcgggggc gagatacaga tccaggcctt cagaatgcga
     7981 aaagcgttcc agtttttcgc gatctgccga acttagcggt gcggtatggc gcaaaatcat
     8041 cagcgtgccg ctggttgcct gtaccagttc aacatgacca agatggcgca tagcttgtaa
     8101 gctgcccaga catgccctga ctttgggcag caatgctcca agttggggcg ctaaaatggg
     8161 gcattgtttg acgtcgacaa tgtcactgga gcccgctttg cgaaacccca tctgaagttg
     8221 ctgtgttttc ggtaagtagt tcagacttaa acgcgcgcgg cggcgatagc cccagggaac
     8281 atcggcgatc acttcagaga catcgtgttt cattaatcgg gcgagtgccg cacttttgct
     8341 tcgctgctgt aaatccacgc tggcgtgttg ttgctgacag ccgccgcata cgccaaaatg
     8401 aggacagcgt ggcgtttcgc gttccgggct atcgcttaac cggcgtacga ctttagcgcg
     8461 ggcatactgt tttttatctt cagtaacagc aacttccgcg ttttcctgcg gcaataatcc
     8521 ggggataaat aacgttttgc cgttatgtcg cgccacgccc tgaccaaaag agtcgaggtc
     8581 gttgactgaa acggttatga tctgacgcgt cgtcgtgcgt cgttttgcag agtagaattg
     8641 cgccattggc gagactttct caatttaaca gtgtgacctt aattgtccca taacggaact
     8701 ccatgaccaa ctacagcctg cgcgcacgca tgatgattct gatcctggca ccgaccgtcc
     8761 ttattggttt attgctgagt atctttttcg tcgtgcatcg ctataacgac ttgcagcgtc
     8821 aactggaaga tgccggtgct agcattattg agccgcttgc agtttctact gaatatggca
     8881 tgagcctgca aaatcgcgaa tctatcggtc agttaataag cgtactgcat cgtcgccatt
     8941 ccgatattgt tcgcgcgatt tcggtttatg atgaaaataa ccgactcttt gtcacctcca
     9001 attttcatct tgatccccca tcaatgcagc tcggcagcaa cgtgccgttt cctcgccagc
     9061 tcactgtcac tcgtgacggc gatattatga tcctccgcac gccgattatt tctgagagtt
     9121 actcccccga cgaatcgccc agtagcgatg ccaaaaatag tcaaaatatg ttgggatata
     9181 ttgcgcttga gctggatctt aaatcggttc gcttgcagca atataaagag atctttattt
     9241 cctgcgtgat gatgctgttt tgtatcggta ttgcgcttat ttttggctgg cgcttaatgc
     9301 gcgatgtaac cggtccgatt cgcaacatgg tgaataccgt cgaccgcatc cgtcgcgggc
     9361 aactcgacag ccgggtggaa ggatttatgc tcggcgagct ggatatgctg aaaaacggta
     9421 tcaactcgat ggcaatgtcg ctggctgctt atcacgaaga gatgcagcac aatatcgacc
     9481 aagcgacgtc cgatctgcgt gaaacgctgg agcagatgga aattcagaac gttgagttgg
     9541 atctggcgaa aaagcgcgcc caggaagcgg cgcgtattaa atccgagttt ctggcaaata
     9601 tgtcacacga gctgcgtaca ccactgaatg gtgttattgg ctttacccgc ctgacgctga
     9661 aaacagaatt aacaccaacg cagcgcgatc acctgaatac gattgaacgt tcggcaaata
     9721 atttgctggc aattattaat gatgttctcg acttctcgaa actggaagca ggtaagctga
     9781 ttctggaaag tattccattc ccactacgca gcacgctgga tgaagtcgtt actctgctgg
     9841 cacattcttc tcacgataaa gggttggaac tgacgctcaa tattaaaagc gacgtgcctg
     9901 ataacgtgat cggcgaccca ctgcgattac agcaaatcat cactaacctg gtggggaatg
     9961 caattaaatt caccgagaat ggcaacattg atattctggt agaaaaacgt gcgctgagta
    10021 ataccaaagt gcagattgaa gtgcagattc gggataccgg cattggtatt cctgaacgcg
    10081 atcaatcgcg cttattccag gccttccgac aggctgatgc cagtatttcc cgccgtcatg
    10141 gtggcaccgg gctggggctg gtgattacac aaaaactggt taatgaaatg ggcggcgata
    10201 tttcgttcca tagccagccg aatcgcggtt caactttctg gttccacatt aatctcgatc
    10261 tgaacccgaa cattattatc gaagggccat ccatccagtg cctcgcaggt aagcgcctgg
    10321 cctatgtcga accaaactcc gcagcagcgc aatgcacgct ggatatttta agtgaaacgc
    10381 cgctggaagt ggtttatagc ccaacgttct ccgcgctgcc tcccgcgcat tacgacatga
    10441 tgttgttagg catcgcggtg accttccgcg agccgctaac aatgcaacat gagcgattag
    10501 cgaaagcggt atcgatgacc gatttcctga tgctggcact tccttgccat gcacaagtca
    10561 atgctgaaaa actcaagcaa gatggtatcg gcgcgtgtct gctgaaacca ttaacaccta
    10621 cgcgcctgtt gcctgccctg acggaatttt gtcatcacaa acaaaacacg cttttgcctg
    10681 taaccgatga aagtaagctg gcaatgacag tcatggcggt tgatgacaac cccgctaacc
    10741 tgaaacttat cggcgcattg ctggaagata tggtgcaaca tgtggaactt tgcgatagcg
    10801 ggcatcaggc agttgaacgg gcgaaacaga tgccgttcga tttgatctta atggatattc
    10861 aaatgccgga catggatggc attcgggcct gcgaactcat ccaccaactc ccgcatcagc
    10921 gacaaacgcc ggttatcgcg gtaacggcgc atgcaatggc cgggcaaaaa gagaagctgc
    10981 ttggcgcagg gatgagcgat tatctggcga aaccgattga agaagagcga ttgcataatt
    11041 tgttgttgcg ctacaagcct ggcagcggta tttcgtctcg cgtcgtgacg cccgaagtca
    11101 acgaaattgt ggtgaacccg aatgcgaccc tcgactggca actggcacta cgccaggcag
    11161 caggaaaaac cgatttagcg cgcgatatgc tgcaaatgtt actcgatttc ctgcctgaag
    11221 ttcgcaacaa agttgaggaa cagctggttg gagaaaaccc ggaaggcctg gttgatttga
    11281 ttcataaact gcatggcagt tgcggctata gcggcgtacc acgtatgaag aatctctgcc
    11341 agctgatcga acaacagcta cgtagcggta ctaaagaaga agatttggag ccggagctgc
    11401 tggaactgtt ggacgagatg gataatgtcg cgcgcgaagc cagcaaaatt ctcgggtaac
    11461 ggacgtcttg tcgagtagca atcggctttt tatgacgctg gtttcagatt ggtgacaaag
    11521 tgcctgtgtt tatgccggat gcggcgttaa cgccttatcc ggcctacaaa accaattaaa
    11581 ttcaatgaat taaaaaatga tgtagacctg atacatagca caacaggcta ttttgcgttt
    11641 agcatcagtc tcaaaccggc tccagataga gccggttttg gttttctgtc ttaacgcacc
    11701 atgcacgggc gcttgttatc gaacgtccag ccaggaatca gatactgcat tcccatcgca
    11761 tcgtcacgcg cgccaagccc gtgtttctga tacagctcat gggctttcat cacttgatcc
    11821 atatcgattt ctacacccag ccccggtttt tctggcacct gtaccagccc gcctttgatc
    11881 tcaaacggtt ctttggtcag gcgctgatta ccttcctgcc agatccagtg cgtatcaata
    11941 gcagtaattt tacccggtgc agcggcggca acatgggtaa acatcgccag ggaaatatcg
    12001 aagtggttgt ttgagtgtga accccaggtc aggccaaatt catggcacat ttgcgccaca
    12061 cgtaccgaac cttgcatagt ccagaaatgc ggatccgcca gcgggatatc aacggattgt
    12121 aacgagagcg tatggcccat ctgccgccag tcggtggcga tcatattggt tgcagtcggc
    12181 aggccagtcg cgcgacggaa ctctgccatc acttcacgcc cggagaaacc ttgctccgca
    12241 ccacacggat cttctgcata agccagcgaa cctttcaggt atttaccaat tttaattgct
    12301 tcgttcagcg accaggcacc gttaggatcg agcgtaatac gcgcctgcgg gaagcgttgc
    12361 gccagtgcca caatagactc ggcttcttct tccccggcca gtacaccgcc tttcagtttg
    12421 aaatcgttga agccatattt ttcatacgcc gcttccgcca ggcgcaccac cgcatccggc
    12481 gtcatcgctt cttcatgacg caggcgatac cagtcgcatg agtcatccgg ctggctttga
    12541 tacggcagag gtgtggcttt gcgattaccg acgaagaaca gataaccgag catttcgact
    12601 tcgctacgct gttgaccatc gcccagcagc gatgccacgt ttacccccag atgctgcccc
    12661 agcagatcca gcattgccgc ttctatcccg gtaactacat gaatagtggt acgcaaatca
    12721 aatgtctgca aaccgcgccc actagcatca cgatcggcaa aagtattacg caccagcgtc
    12781 agaacgtttt tgtattcacc cagcgtttta cctaccacca gcggaatcgc atcttccagc
    12841 gttttacgga ttttctcgcc gccgggaatt tcccctacgc cagtgtgacc ggaattatct
    12901 ttgataatca caatattacg cgtgaagaac ggtgcgtgtg caccactcag attcatcagc
    12961 atactgtcat gacccgccac cgggatgacc tgcatttcag taacaacagg cgtcgtaaat
    13021 tgagaactca tatttatgtc ctttttcaga atcaatgacg gccgaaaacg ggacgtttac
    13081 ggtcaaaggt ccagccgggg atcaggtact gcatcggacc tgcgtcgtta cgcgcaccgc
    13141 aaggcagacg tttataggcc tcatgtgcct tttgtacctg ttcccagtcc agttccacgc
    13201 ccagaccggg cgcatcagga acggcaattt ttccgttttt aatctccagc ggatttttgg
    13261 tcaggcgaca atcgccctcc tgccaaatcc agtgggtatc gatagcggta ggattacccg
    13321 gtgccgccgc gcccacatgg gtaaacatcg ccagagagat atcgaaatgg ttgttggaat
    13381 ggcagcccca ggtcagcccc cagtcgtcgc aaagctgcgc cacgcggact gcaccggaaa
    13441 gcgtccagaa gtgcggatcg gcaagtggaa tatctaccgc attgagcatc accgcatgac
    13501 ccatttcgcg ccagttggtg gcgatcatgt tagtcgcgac gggcaagccg gtcgcccgtc
    13561 gaaactccgc catcacttca cgtccggaga agccctgttc tgcgccgcat ggatcttcgg
    13621 cataggtaag aacatcattc agccctttgc ataaagaaat ggcttcatca agcagccatg
    13681 caccgttggg atcaacggta atccgcgcat ccgggaagct tttcttcaat gcacgaacag
    13741 tgtcgatttc ttgctcgcca ggtaacaccc cgcccttaag tttgaaatct ttaaagccat
    13801 agcgatcctg tgaggcttcc gccagacgca caacggcttc gctgttcatc gctttctgat
    13861 ggcgcaactg ataccactca tggttgcccg gcgtattttc cagataagga agatcggttt
    13921 tggtccgatc accgatataa aacagataac cgaggacggt aatagtctcg cgttgcttgc
    13981 ctggccccaa cagttcgcag accggaacat tcagcgcctt acctagcagg tcaagcaagg
    14041 cggcttccag cgccgccacg gcattaacgc gcaattcaaa agtccaggca cctttgccga
    14101 aggtatcaaa atcggctgcc tgattacctt tatgcacctg ctgaaccact ttattcaggc
    14161 gcgcaacttc ctggcccaga accatcggaa tagcatcgac cagtgtttga taaatcacct
    14221 ctccgcccgg cgcttcacca atgccggtat gcccggcgtt atcggtgagt accacgatat
    14281 tgcgggtgaa gtatgcgtta tgcgcaccac caatattgag caacatgctg tcatgtccgg
    14341 cgaccggaat gactttcata tcagtaataa caggactgga ttgtgtcgcc atcttattgc
    14401 cccgcgacag atttcagctc gatacgcttg atatcgccca ccagcaccag gtagctcagt
    14461 accgcgatta gggcatgaac accaacataa atcagcgccc cattaaacga gccagtcgtg
    14521 ccaacgatat aaccaattgc gattggcgtg acgatgccag aaatgttacc gaacatgttg
    14581 aacaggccac cggaaagacc gctgatctct tttggcgcgg tatctgccat tactgcccaa
    14641 cccagcgccc cgatgccctt accgaagaag gccagcgcca taaagccgat gatcatccac
    14701 tcaacgttga cgtagttgca gaacaccatc accatcgaca gcaacatgcc cattacgatc
    14761 ggtgttttac gcgcaatgtt cagcgatccc gtgcggcgca tcagccagtc ggaaataatt
    14821 ccacccagca caccgccgat aaaaccgcaa accgccggaa cggaagccac aaagcccgct
    14881 ttcagaatcg acatcccacg cgcctgcacc agataaaccg gaaaccaggt aatgaaaaag
    14941 taagtcaggg cgttgataca gtactgaccg atataaacgc cgatcatcat ccgtgacccg
    15001 agcagctgtt tgatctgccc ccacttcacg ctgaacggca ctttaacttt ggtgttttgc
    15061 tgatccatat tgatcagcgc gccacccgcg gcgatatact ccagctcttt ctgatttacc
    15121 cccggatgtt gatttggctc gtggatgact ttcaaccaga taaagctgat gacaatgccc
    15181 agaccgccca taaagaagaa gacgtgtgac cagcccactt catgcgtcag ccagcccata
    15241 atcggcgcga agatcaccgt tgcgaagtat tgagcggagt taaaaatcga caccgccgtt
    15301 cccctttcct gcgccggaaa ccaggccgca acaatgcgac tgttgccggg gaaagatggc
    15361 gcttcagcaa gcccgaccag gaagcgcaga gtaaacaggg caacgataat gccgaatcca
    15421 ctaaagatat cgacgaagcc ttgcagcaag gtaaacatcg accagataaa gatcgaccag
    15481 aagtagacgc gttttgaacc aaaacgatcc agcaaccaac caccagggat ctgcccgata
    15541 acataagccc atgagaaggc agagaacaca tagcccattc ccacgggatc aaggccgata
    15601 tctttggcca tttccgaacc ggcaatagaa agcgtagcgc ggtcgccgta gttgaaggat
    15661 gtgacgataa acaacatcac cactatccag taacgagcat tcgtgcgttt ttccacactg
    15721 ctcgcagcct gacttaaaga actcattgtt gcactcctga aaattcgcgt tagccacgct
    15781 cactctggac tgcgacatcg ccaggaaatc agaggtgacg tagggtgttt tttgccgttt
    15841 ttataggtcg ttcgccgaat acggcgcgtg tttatatctg gcaatagcag tataaaaagc
    15901 gcgccatagc ggctcaccgt gcaacaacac aacattaatg cgttcaatga ggcccgattt
    15961 tggcattagc cctggacggt ggaatccact tcacggaaat gaaaacaaga acaagaaagg
    16021 aagggtaaaa acgaagaaat aaagaagagt atgaaatgga tcgcttgact ctaggcaaac
    16081 gccagtaaaa atccgcgcta tgaagcagtt tttactggca tttgcctgaa aagattcgat
    16141 tcagcaccgc taaaacgaca tttaccgctc gctgacatat caggacaaca gcgtgcccca
    16201 ctgttcgacc cacggatttg attccgtttc cggttccggg ttctcactgg cgtcaatcaa
    16261 cagcatttcg ccaacccgct gagcgctctg ttcctgcaac aaggcatcga actgtttgcc
    16321 gccattgcaa aaatttacat aactgctgtc gccgagcgca atcacgccat aacgcagatt
    16381 cggctggaag cccagactat ctttgattcc ctgaaagagc ggcacaatgc tatcaggaag
    16441 gtcgccctgc ccggtcgtgg acgtaaccac cagaacatac ttatcctgat agggcagcca
    16501 gtcgcttaat tcaggatctt caaataccgt tgctttgtgg ccctgtgcgg tcagaattgc
    16561 ttctgcttct tccgccacta acagcgaatt tccgtacatg gtgccgacaa aaataccaat
    16621 ttccgccata ccgttttctc cctggattag gaacttatct ctccatcctg acccgatggc
    16681 gcactgaact caaccctttc attttcagga agcagaccgc gccagccaaa ttgtgataac
    16741 gcctgcatcc aggtgtcgtc caggcccgcg tgaatagtca gcggctcgcc agtaaaagga
    16801 tgcgtcaatg acaactgact ggcatgtagc attaaccgct ggaggccaaa atgctcagca
    16861 ccgctgcgat tctggcgtaa atcgccatgt ttgctatcgc caataatcgg atgacgcaaa
    16921 tgggcaagat gtcggcgaag ctgatgtttg cgtccggttt tcggctccag ttccaccagg
    16981 ccgtagcgcg tggtcgggta acgtccggtc gctaccggca tttctacggt cgccagaccg
    17041 cgataatgcg tcactgctgg ctgcgggcct ttatcttcgc gggcaaattt atcagcgatt
    17101 ttgtccagtt cttccaccag tggataatcc aacaccgctt cttccatcaa ccagccgcgc
    17161 acaatcgcat ggtaacgttt ctggatttgg tgctgttcaa actgttgtgc cagcagccgt
    17221 ccggcctcgc tggataatcc catcaacaac acaccagaag tgggtcggtc cagacgatga
    17281 gcagtaaaaa catgctgtcc tatctggtca cgcacggttt gcatgaccac tactttctcg
    17341 tcgcgatcca gccagctacg gtgaaccagc cagccggagg gtttatttac cgcaaccagc
    17401 cattcatcct gatagagtat ttccagcatt agctcgcatc atccgcaaaa agagcatcca
    17461 gtttttccaa ctcagccaga ataagcgcgc gttgcggatg gtccgtcgcc agcgccattt
    17521 cataataggg tgcaacagca aaagcgcccg gtaacggctg gttgttattt aacaaatcgt
    17581 gcattcgcgg gatcagcccc cactgcaacc actccagtgg ttccatggtg tccataaaga
    17641 acggttgggt actattaaat tgatgcggct ggggttcatc gtttcgccag tgctgatgtt
    17701 cacgcagtaa cgcttcaagc gcctgcaact ggagacgaac gcggtcatga gtggtcataa
    17761 agcaacctca ataagaaaaa ctgccgcgaa ggatagcagg aataaaaaaa gggagcactg
    17821 tattcacagt gctcccggtt cgtttcgcag cattccagct acttttgttg ctccctgctc
    17881 atccttgaca acttttcctc tggccttgcg gccaatcgtt catcctgaac tattgcttcc
    17941 tgctcacacc accccgatgt gatacttcat cctgaagtgt ccctggcctt cctgacccac
    18001 cgaatcatcc tgaccggttc tcattctcca tcctggaggt gtcctttaac gcgtcctgcg
    18061 tcatcctctt cgcttcatcc agaagccttt ccctgaaaca ccatcctggt gtgtcctgca
    18121 gaagtgtcat catcctgatg ttcacttcgt tgtctgactc cctgtcgacg aagatagaat
    18181 cgtctttttc gcgaagtctt acaaggcgct tacagacaat gccttaagga aaatttcata
    18241 tgaaagttaa gaattaacaa tcaacatttt gattttaaat gaaaataacg attcttatct
    18301 ttttaagtat tcgcttattt cctggcgatc tcctacagag tctgtaagag atctctcaca
    18361 accagatggg gtatttggat tacagaagag gcttaagttg attaaggaat tccgcaagat
    18421 ttgaggctaa atgcgtccgt ttacgtgttc ccagcgtctc tttgcacact tcaccggaga
    18481 gattacacac cgagatgacc tctaactctt cttccagcgt cgcgataaat agcgtgggcg
    18541 gcagttttag ccttttctga gtcaccagat gaccaatcag attttcctgc acccgacgga
    18601 agtcgtcttc actccaggtt tgcagcaatg tcagtttgat atcgccaaac tgcgcgtgca
    18661 tatccccggc aaactgagtc gtataaaagg tatgaattgt gggttgtatc acaatatcaa
    18721 aagcgcgttc aaccgcgttt acattttgtt ccccggtaaa cggctggggt tgccagtata
    18781 cggcatcttc ggtagttgaa ataatgcacg gtgaaggaac accgtacagt tcctcactta
    18841 acggccagct tttatgctct tcatgccacg catcgcagta gcgtgcagta aaatctttca
    18901 gggcttgtgc ggtcaaatcg tccactaatt tctctcttca cttaaaccag atacacttgt
    18961 cgtttagttt atctggttta tgacggtgaa acatgtcttc ttatgcaaac catcaggcac
    19021 ttgcgggcct gactcttgga aaatcaaccg attaccggga tacctatgac gccagcctac
    19081 tgcaaggcgt tccacgcagc ctgaatcgcg acccgctggg tctgaaagcg gataacctgc
    19141 cttttcacgg tacggatatc tggacgctgt atgaactttc ctggctgaat gcgaaaggtt
    19201 tgccgcaggt cgctgtcggt catgttgaac ttgattacac cagcgtaaat ctgattgagt
    19261 cgaagagttt taagctctat ctcaacagtt ttaaccagac gcgttttaat aactgggatg
    19321 aggtgcgcca gacgctggag cgcgacttaa gcacttgcgc tcagggtaag attagcgtgg
    19381 cgttatatcg tcttgatgaa ctggaaggcc agccgatagg tcatttcaat ggcacttgca
    19441 ttgatgacca ggatatcact atcgataact atgaattcac tactgactat ctggagaatg
    19501 ccaccagcgg tgaaaaagta gtggaagaga cgctcgtcag ccacctgctg aaatcaaact
    19561 gcctgatcac ccatcaacca gattggggtt cgatccaaat tcagtatcgt ggacgccaaa
    19621 ttgacagaga aaaactgctg cgttacctgg tctcattccg tcatcacaac gagttccacg
    19681 aacagtgcgt ggaacgcatc tttaatgacc tgttacgctt ctgccagcca gagaaattga
    19741 gcgtttacgc acgttatacc cgtcgtggcg gtctggacat taacccgtgg cgcagtaata
    19801 gcgattttgt cccatcgacc acaagactgg ttcggcaata aattttttct caattttgcg
    19861 tgctggattc acgcagaagg ttgtgaaagg tcatcaggca gggctattgt aatcaaaggg
    19921 aatgacgata ttcgtcccat aaggagtttt tcttgattac acatattagc ccgcttggct
    19981 ccatggatat gttgtcgcag ctggaagtgg atatgcttaa acgcaccgcc agcagcgacc
    20041 tctatcaact gtttcgcaac tgttcacttg ccgtactgaa ctccggtagt ttgaccgata
    20101 acagcaaaga attgctgtct cgttttgaaa atttcgatat taacgtcctg cgccgtgaac
    20161 gcggcgtaaa gctggaactg attaatcccc cggaagaggc ttttgtcgat gggcgaatta
    20221 ttcgcgcttt gcaggccaac ttgttcgcgg ttctgcgaga cattctcttc gtttacgggc
    20281 aaatccataa taccgttcgt tttcccaacc tgaatctcga caactccgtc cacatcacta
    20341 acctggtctt ttccatcttg cgtaacgctc gcgcgctgca tgtgggtgaa gcgccaaata
    20401 tggtggtctg ctggggcggt cactcaatta acgaaaacga gtatttgtat gcccgtcgcg
    20461 tcgggaacca gctgggcctg cgtgagctga atatctgcac cggctgtggt ccaggagcga
    20521 tggaagcgcc gatgaaaggt gctgcggtcg gacacgcgca gcagcgttac aaagacagtc
    20581 gttttattgg tatgacagag ccgtcgatta tcgccgctga accgcctaac ccgctggtca
    20641 acgaattgat catcatgccg gatatcgaaa aacgtctgga agcgtttgtc cgtatcgctc
    20701 acggcatcat tatcttccct ggcggtgtgg gtacggcaga agagttgctg tatttgctgg
    20761 gaattttaat gaacccgacc aacaaagatc aggttttacc attgatcctc accggcccga
    20821 aagagagcgc cgactacttc cgcgtactgg acgagtttgt cgtacatacg ctgggcgaaa
    20881 acgcgcgccg ccattaccgc atcatcattg atgacgccgc tgaagtcgct cgtcagatga
    20941 aaaaatcgat gccgctggtg aaagaaaatc gccgtgatac aggcgatgcc tacagcttta
    21001 actggtcaat gcgcattgcg ccagatttgc aaatgccgtt tgagccgtct cacgagaata
    21061 tggctaatct gaagctttac ccggatcaac ctgttgaagt gctggctgcc gacctgcgcc
    21121 gtgcgttctc cggtattgtg gcgggtaacg taaaagaagt cggtattcgc gccattgaag
    21181 agtttggtcc ttacaaaatc aacggcgata aagagattat gcgtcgtatg gacgacctgc
    21241 tacagggttt tgttgcccag catcgtatga agttgccagg ctcagcctac atcccttgct
    21301 acgaaatctg cacgtaatct cccctcccc
//
LOCUS       DBKEKI010000060        20545 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.060, whole genome shotgun sequence.
ACCESSION   DBKEKI010000060 DBKEKI010000000
VERSION     DBKEKI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.060"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="ACUQPX_002263"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="ACUQPX_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HHV9056965.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="ACUQPX_002264"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="ACUQPX_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HHV9056966.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="ACUQPX_002265"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="ACUQPX_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HHV9056967.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACUQPX_002266"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACUQPX_002266"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HHV9056968.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACUQPX_002267"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACUQPX_002267"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HHV9056969.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGREPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="ACUQPX_002268"
     CDS             4449..4814
                     /locus_tag="ACUQPX_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HHV9056970.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4964..5482
                     /locus_tag="ACUQPX_002269"
     CDS             4964..5482
                     /locus_tag="ACUQPX_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HHV9056971.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="ACUQPX_002270"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="ACUQPX_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="ACUQPX_002271"
     CDS             6713..7195
                     /locus_tag="ACUQPX_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HHV9056972.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="ACUQPX_002272"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="ACUQPX_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HHV9056973.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="ACUQPX_002273"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="ACUQPX_002273"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HHV9056974.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="ACUQPX_002274"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="ACUQPX_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HHV9056975.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="ACUQPX_002275"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="ACUQPX_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HHV9056976.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="ACUQPX_002276"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="ACUQPX_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HHV9056977.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="ACUQPX_002277"
     CDS             10939..11730
                     /locus_tag="ACUQPX_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly family protein"
                     /protein_id="HHV9056978.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="ACUQPX_002278"
     CDS             11751..13037
                     /locus_tag="ACUQPX_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HHV9056979.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="ACUQPX_002279"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="ACUQPX_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HHV9056980.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="ACUQPX_002280"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="ACUQPX_002280"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HHV9056981.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="ACUQPX_002281"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="ACUQPX_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HHV9056982.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="ACUQPX_002282"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="ACUQPX_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HHV9056983.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="ACUQPX_002283"
     CDS             complement(16985..17275)
                     /locus_tag="ACUQPX_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HHV9056984.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="ACUQPX_002284"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="ACUQPX_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HHV9056985.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="ACUQPX_002285"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="ACUQPX_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HHV9056986.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="ACUQPX_002286"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="ACUQPX_002286"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="ACUQPX_002287"
     CDS             19137..20378
                     /locus_tag="ACUQPX_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HHV9056987.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKKRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5304 a         5364 c         4825 g         5052 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcactaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgctaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacaggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggctcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acaccccctt tgaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaggggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aggagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaaccta gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cacccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgata tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgtcttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcgacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccaa ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctggtgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtgccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg cgagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgcta
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttatttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacccggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgccccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagaaacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 tcggtaatgc tgccaactta ctgat
//
LOCUS       DBKEKI010000061        20441 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.061, whole genome shotgun sequence.
ACCESSION   DBKEKI010000061 DBKEKI010000000
VERSION     DBKEKI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.061"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..214)
                     /locus_tag="ACUQPX_002288"
     CDS             complement(<1..214)
                     /locus_tag="ACUQPX_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9056988.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKE"
     gene            complement(222..932)
                     /locus_tag="ACUQPX_002289"
     CDS             complement(222..932)
                     /locus_tag="ACUQPX_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHV9056989.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1148..2971)
                     /gene="typA"
                     /locus_tag="ACUQPX_002290"
     CDS             complement(1148..2971)
                     /gene="typA"
                     /locus_tag="ACUQPX_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HHV9056990.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            3344..4753
                     /gene="glnA"
                     /locus_tag="ACUQPX_002291"
     CDS             3344..4753
                     /gene="glnA"
                     /locus_tag="ACUQPX_002291"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HHV9056991.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            4926..5975
                     /gene="glnL"
                     /locus_tag="ACUQPX_002292"
     CDS             4926..5975
                     /gene="glnL"
                     /locus_tag="ACUQPX_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HHV9056992.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            5987..7396
                     /gene="glnG"
                     /locus_tag="ACUQPX_002293"
     CDS             5987..7396
                     /gene="glnG"
                     /locus_tag="ACUQPX_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HHV9056993.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            7508..7618
                     /locus_tag="ACUQPX_002294"
     CDS             7508..7618
                     /locus_tag="ACUQPX_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HHV9056994.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(7847..9220)
                     /gene="hemN"
                     /locus_tag="ACUQPX_002295"
     CDS             complement(7847..9220)
                     /gene="hemN"
                     /locus_tag="ACUQPX_002295"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HHV9056995.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(9409..9918)
                     /gene="yihI"
                     /locus_tag="ACUQPX_002296"
     CDS             complement(9409..9918)
                     /gene="yihI"
                     /locus_tag="ACUQPX_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HHV9056996.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDVLLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(10037..10203)
                     /locus_tag="ACUQPX_002297"
                     /pseudo
     CDS             complement(10037..10203)
                     /locus_tag="ACUQPX_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10500..11132
                     /gene="yihA"
                     /locus_tag="ACUQPX_002298"
     CDS             10500..11132
                     /gene="yihA"
                     /locus_tag="ACUQPX_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HHV9056997.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(11514..14300)
                     /gene="polA"
                     /locus_tag="ACUQPX_002299"
     CDS             complement(11514..14300)
                     /gene="polA"
                     /locus_tag="ACUQPX_002299"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HHV9056998.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            14664..15596
                     /locus_tag="ACUQPX_002300"
     CDS             14664..15596
                     /locus_tag="ACUQPX_002300"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HHV9056999.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(15637..17067)
                     /locus_tag="ACUQPX_002301"
     CDS             complement(15637..17067)
                     /locus_tag="ACUQPX_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HHV9057000.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNDAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKNRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(17222..17848)
                     /gene="dsbA"
                     /locus_tag="ACUQPX_002302"
     CDS             complement(17222..17848)
                     /gene="dsbA"
                     /locus_tag="ACUQPX_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HHV9057001.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(17865..18851)
                     /gene="srkA"
                     /locus_tag="ACUQPX_002303"
     CDS             complement(17865..18851)
                     /gene="srkA"
                     /locus_tag="ACUQPX_002303"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HHV9057002.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(18928..19197)
                     /locus_tag="ACUQPX_002304"
     CDS             complement(18928..19197)
                     /locus_tag="ACUQPX_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HHV9057003.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            19267..19851
                     /gene="mobA"
                     /locus_tag="ACUQPX_002305"
     CDS             19267..19851
                     /gene="mobA"
                     /locus_tag="ACUQPX_002305"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HHV9057004.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            19833..20360
                     /gene="mobB"
                     /locus_tag="ACUQPX_002306"
     CDS             19833..20360
                     /gene="mobB"
                     /locus_tag="ACUQPX_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HHV9057005.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         4968 a         5164 c         4952 g         5357 t
ORIGIN      
        1 gttctttatc gtccatatta ccaagagaaa aacgctcgcc atccagaaca tggattttga
       61 cgccacgtag attattttca cttgcgatat caagtaaatc agcaggcaac actctttcca
      121 gccgcatatt taaatgaaag gcataagcat gtaaataaag aggaagagtg tcgacacgtt
      181 gtagaatctt tcttgcgtta tttattgtca ccataaaaca cctacagatt aatgatgagg
      241 tctacggcct caaatcgcca atattcaata tctatctgga caatattgcc ttgctggtcg
      301 gcgcgatgct tttcaattaa gatcgctggc atacccgcag aaccgccaag atacttgctg
      361 atatcgcccg gcattctgac gggtttgaaa atcaattttt ttactaccgt ctctttttgg
      421 tagcactttt cccagactgc agaaaatgag tggttttcaa gttgttcaat aaaacccgga
      481 gcaacttcag gattaatata tgtttcgtga tagaaaactt tatgtccttc cagcgccccc
      541 cagccggtga tgcgatagag ttcagttgat ggcgtcactg caatcagtgg cgcgagcgtc
      601 tcgggaatat cgctggtacg gtttttctcg gtaaaccccc aagaaggctc tcgcccttgc
      661 tcaattgcgg cccgctgaaa gctcgccgac agctccggac tgtaattaaa tcgcggctgg
      721 gtcacgaacc accccttacg atccttacgg aagattttgg attctgcctc gaggttcaac
      781 aacgcctggc gcagcgtcat acgtttaatg cccagcagtt ctcccagctc tctttctgaa
      841 gggagttttc cacccggcgt ggtgatgcca tctttcaacc accgatccag tttctctttt
      901 gcattttcta cggtggattg attctccgcc attgtttaat ccttttgatt taaaccagat
      961 gaagcatctt acgcttacgc caaaaaagaa agcaattaac ggtgtatgac tttgcagata
     1021 attgtgacaa agctctctat tgacgtaatc acagacttat aagggagata ctcgcattgg
     1081 tgtgcgctca cgacaggtaa aaaaaacctg ccatcgctgg caggtttttt atgactaaaa
     1141 aacgaaatta atcgtctttc ggtgcgcggt tggcgcggcg acgatcgttt tccgtcaggt
     1201 gacgtttacg aatacggata gaggtcggag tcacttctac cagttcgtca tcatcgatga
     1261 actccagagc ttgttccaga gtcatgcgga taggcggaac cagaacaaca gcttcgtcag
     1321 taccggaagc acgcatgttg gtcagtttct taccggtcag gcagtttaca gtcaggtcgt
     1381 tagagcggct atgaataccg ataatctgac cttcgtaaac ttctgcaccg tgaccgagga
     1441 acagcttacc gcgatcctgc agaccgaaca gcgcgaacgc gacagcttta ccctgaccgt
     1501 tagagatcag tacaccgttc tgacgctgac ccacttcgcc cggacgtacg tcgtcgtagt
     1561 ggctgaaggt ggagtacagc agaccagtac cggaagtcat ggtcatgaac tcagaacgga
     1621 agccaatcag accacggctt gggatcacgt agtcgagacg tacgcggcct ttaccgtctg
     1681 gattcatgtt tttcaggtcg cctttacgtt cgcccagcgc ctgcatcaca gaaccctgat
     1741 gctgttcttc aacgtccagc gtcacgtttt catacggctc ttgtttacga ccgtcgattt
     1801 cacggaagat tactttcgga cgggataccg ccagttcgaa accttcacga cgcatgtttt
     1861 cgataagaac agagaggtgc agttcaccac gaccagaaac gcggaacgca tcggcgtctt
     1921 cggtttcttc tacgcgcagc gcaacgttgt gtaccagttc tttgttcaga cgatcgagga
     1981 tctgacgaga cgttacgaac ttaccttctt taccgcagaa cggcgaggtg ttaacgcaga
     2041 agaacataga aacggtcggc tcatcaacgg agagtgccgg cagcgcttca acgttttgcg
     2101 tgtcgcaaac ggtgtcagaa atgttcagtt cgcccaggcc cgtgatcgcc acgatatcgc
     2161 cagcttccgc cagatcggtt tcgatacgtt ccagaccgag gtggcccagc actttaccga
     2221 ctttcgcgtt acgggttttg ccttcgctat cgatgatagt aacctgctgg ttcggcttca
     2281 ctttaccacg cttgatgcgg ccaatgccga taacgccaac atagctgttg taatcgagct
     2341 gagaaatctg catctggaac ggaccgtcaa ggtcaacgtc cggcgcagga acgtggtcaa
     2401 caatcgcctg atacagcggg gtcatgtctt ccgccatatc ttcgtggtcc agacccgcga
     2461 taccgttcag cgcagaagcg taaacgatcg ggaagtccag ctgctcgtcg gtcgcgtcga
     2521 ggttaacgaa cagatcgaat acctgatcca caacccaatc aggacgcgcg ccagggcggt
     2581 caactttgtt gataacaaca atcggcttca ggccgtaagc aaacgctttt ttggttacga
     2641 agcgcgtttg cggcatcggg ccgtcaaatg cgtcaaccac cagcagcact gagtctacca
     2701 tggacattac acgttcaact tcaccaccga agtcggcgtg ccccggggta tcaacgatgt
     2761 tgatacggta atcattccat ttgatagcgg tgtttttcgc gaggatggta atcccacgct
     2821 ctttctccaa atcgttggag tccatcacgc gctcttgggt ttcggcacga gagtcgaacg
     2881 taccggattg ttggagcagc ttgtctacca gggtggtttt accatggtct acgtgcgcga
     2941 tgatggcgat attacgcaat ttttcgatca caactttgcc tcaggcatta gaaatagcgc
     3001 gttattgtac acggattaat cgcactacaa aacaggatca caaacatcct ccgcaaacaa
     3061 gtattgcaga gtccctttgt gatcgctttc acggagcata aaaagggtta tccaaaggtc
     3121 attgcaccaa tatggtgctt aatgtttcca ttgaagcact atattggtgc aacattcaca
     3181 tcatggtgca gcccttttgc acgatgttaa gcatgataac gccttttagg ggcaatttaa
     3241 aagttggcac agatttcgct ttatcttttt tacggcgaca cggccaaaat aattgcagat
     3301 ttcgttacca cgacgacaat gaccaatcca ggagagttaa agtatgtccg ctgaacacgt
     3361 actgacgatg ctgaacgagc acgaagtgaa gtttgttgat ttgcgcttca ccgataccaa
     3421 aggtaaagaa caacacgtca ctatccctgc tcatcaggtg aatgctgaat tcttcgaaga
     3481 aggcaaaatg tttgacggct cctcgattgg cggctggaaa ggcattaacg aatccgacat
     3541 ggtgctgatg ccagacgcat ccaccgcagt gattgacccg ttcttcgccg actccaccct
     3601 gattatccgt tgcgacatcc ttgaacctgg caccctgcaa ggctatgacc gtgacccgcg
     3661 ctctattgcg aagcgcgccg aagattacct gcgttccact ggcattgccg acaccgtact
     3721 gttcgggcca gaacctgaat tcttcctgtt cgatgacatc cgttttggat catctatctc
     3781 cggttcccac gttgctatcg acgatatcga aggcgcatgg aactcctcca cccaatacga
     3841 aggtggtaac aaaggtcacc gtccggcagt gaaaggcggt tacttcccgg ttccgccggt
     3901 agactctgct caggatattc gttctgaaat gtgtctggtg atggaacaga tgggtctggt
     3961 ggttgaagcc catcaccacg aagtagcgac tgctggtcag aacgaagtgg ctacccgctt
     4021 caataccatg accaaaaaag ctgacgaaat tcagatctac aaatatgtcg tgcataacgt
     4081 agcgcaccgc ttcggtaaaa ccgcgacctt tatgccaaaa ccgatgttcg gtgataacgg
     4141 ctccggtatg cactgccaca tgtctctgtc taaaaacggc gttaacctgt tcgcaggcga
     4201 caaatacgca ggtctgtctg agcaggcgct gtactacatt ggcggcgtaa tcaaacacgc
     4261 taaagcgatt aacgccctgg caaacccgac caccaactct tataagcgtc tggtcccggg
     4321 ctacgaagca ccggtaatgc tggcttactc tgcgcgtaac cgttctgcgt ctatccgtat
     4381 tccggtggtt tcttctccga aagcacgtcg tatcgaagta cgtttcccgg atccggcagc
     4441 taacccgtac ctgtgctttg ctgccctgct gatggccggt cttgatggta tcaagaacaa
     4501 gatccatccg ggcgaagcca tggacaaaaa cctgtatgac ctgccgccag aagaagcgaa
     4561 agagatccca caggttgcag gctctctgga agaagcactg aacgaactgg atctggaccg
     4621 cgagttcctg aaagccggtg gcgtgttcac ggatgaagcg atcgatgcgt acatcgctct
     4681 gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcat ccggtagagt ttgagctgta
     4741 ctacagcgtc taatagttga agttgtacta cccggcgcac aacgccggga tttagttgcc
     4801 gtggaaactt tcagcccatc tctgcatggg cttttttctc cgtcaattct ctgatgcttc
     4861 gcgcttttta tccgtaaaaa gctataatgc actaaaatgg tgcaacctgt tcaggagact
     4921 gctttatggc aacaggcacg cagcccgatg ctgggcagat cctcaactcg ctgattaaca
     4981 gtattttgtt aattgatgac aatctggcga tccattacgc caaccccgcc gcgcaacaac
     5041 tgctcgccca aagctcccgc aaattgtttg gtacgccgtt accggaactg ttgagctact
     5101 tctcattaaa tatcgagctg atgcaagaaa gtctggaggc ggggcaaggc tttaccgata
     5161 acgaagtgac gctggtcatc gatggtcgct cgcatatcct ttctgtgacg gcccagcgta
     5221 tgccggacgg catgatcctg ctggagatgg caccgatgga taaccagcgc cgcttaagcc
     5281 aggaacaact acaacatgct caacaagtag ctgcccgtga tttagtgcgc ggcctggcac
     5341 atgagattaa aaatccgctt ggcggtttac gtggcgcggc gcagttactc agcaaagcgt
     5401 tacctgaccc atcactactc gaatatacca aagtgattat cgaacaggcg gaccggctgc
     5461 gaaatttggt cgaccgtctg ttggggccgc agctgcccgg tacgcgcgtt accgaaagta
     5521 ttcacaaagt ggctgaacgc gtggtaacgc tggtgtcgat ggaactgccg gacaacgtgc
     5581 ggttgattcg tgattacgat cccagcctgc cggaactggc gcacgacccg gatcaaattg
     5641 aacaggtctt gctgaatatt gtgcgcaatg cgctacaggc gctggggccg gaaggcggtg
     5701 aaatcattct gcgtacccgc accgcgtttc aactgacctt acacggcgag cgctaccggc
     5761 tggcggcgcg gattgatgtg gaagataacg ggccgggcat tccgcctcat ttgcaggata
     5821 cgctgtttta cccgatggtc agcggccgcg aaggtggcac cgggcttggc ttatccatcg
     5881 cccgtaattt gattgatcag cattcaggca aaattgaatt taccagttgg ccaggtcata
     5941 ccgagttctc ggtttacctg cctatcagga aataaaggtg acgtttatgc aacgagggat
     6001 agtctgggta gtcgatgacg atagttccat ccgttgggtg cttgaacgtg cgctcgctgg
     6061 cgcgggttta acctgtacga catttgagaa cggcgcggaa gtactggagg cgctggcgag
     6121 caaaacgccg gatgtgctgc tttcagatat ccgtatgccg ggaatggacg ggctggcgct
     6181 gctcaagcag attaaacagc gccatccaat gcttccggtc atcattatga ccgcacattc
     6241 cgatctggat gctgccgtca gcgcctatca acaaggggcg tttgattatc tgcccaaacc
     6301 gtttgatatc gacgaagcag tggcgctggt tgagcgcgct atcagtcatt accaggaaca
     6361 gcagcagccg cgtaatattc agcttaacgg cccaacgacc gatatcatcg gcgaagcgca
     6421 agccatgcag gacgtgttcc gtattatcgg tcggctttcg cgttcttcta ttagcgtgct
     6481 gattaacggc gaatccggca ccggtaaaga actggtcgct catgccctgc atcgccacag
     6541 tccgcgcacc aaagcgccgt ttatcgcgct gaatatggca gctatcccaa aagatttgat
     6601 cgaatcagaa ctgtttggcc acgagaaagg cgcgtttact ggcgcgaata ccattcgtca
     6661 ggggcgtttt gaacaggccg atggcggcac gttatttctc gacgaaattg gtgatatgcc
     6721 gctggatgtg cagacgcgtt tgctgcgcgt gctggcagac ggtcagtttt atcgcgttgg
     6781 cggctatgcg ccggtgaaag tggatgtgcg gattatcgct gccactcacc agaatcttga
     6841 acagcgggtg caggaaggta agttccgtga ggatctgttc caccgcctga acgttatccg
     6901 cgttcatctg ccgccgctgc gcgaacgtcg ggaagatatt ccccgtctgg cgcgccattt
     6961 tttacaggtt gccgcgcgcg aactgggcgt agaagcgaag ttgctgcatc cggaaaccga
     7021 agctgctctg acgcgtctgg cgtggccagg caacgtgcgc cagctggaaa acacctgccg
     7081 ctggctaacg gtgatggccg ccgggcagga agtgttgatt caggatttgc ccggcgaact
     7141 gtttgaatca acggttgcgg agagtacttc gcaaatgcaa ccggacagtt gggcgacact
     7201 tttagcgcag tgggcagaca gagcgctgcg ttccggtcat caaaatctgc tttccgaagc
     7261 gcagccagag ctggagcgga cgttactgac gaccgcgttg cgacatacgc aggggcataa
     7321 acaggaagcg gcgcggctac tcggctgggg ccgcaacact ctgacgcgta agttaaaaga
     7381 gctggggatg gagtgattca cggcttgtgt gtaaagattg attattgagc gcaaattgct
     7441 ggtattttgc gctttactgt tccgataagt tcagtatgat cttgcccgga aaacggggag
     7501 agtcattatg ctggaatcaa taattaatct ggtatcgagt ggcgcagttg acagccacac
     7561 accgcaaact gccgttgctg ccgtgctgtg tgccgcgatg attgggctgt ttagctgaag
     7621 ctcgttgttt atgccggatg cggagaaagt cgtaggcctg ataagacgcg accagcgtcg
     7681 catcaggcat catgctcaga tgcctgatgc ggcgtaaacg ccttatccgg cctacaaaag
     7741 caataatttc aacaggtttt gttgcccagc gcatcaggca atcttgttct cagcacattg
     7801 atggcgcttt cgttttctac tttgtaaacg aagcgccatt cactctttaa atcacccgtg
     7861 agaactgctg catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc
     7921 ggatcagcaa gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc
     7981 catcttttgc taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca
     8041 aatcccactg tttctcaaga ggggcgtaat ccagacggaa gttgcagatg agcgacttaa
     8101 tcacatcgcg gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc
     8161 cttgttcatc cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt
     8221 cgccaatcat gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg
     8281 tgtagccctg gaagttacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat
     8341 caggacgggc aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga
     8401 tggtttcctg caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt
     8461 tgcgctgagc agcaaaaatg gtcggcagat gcgcgtagtt aaaaacgctc agacgatcgg
     8521 ggttcagctc cgccacacgt ttcagggtaa aggcgaaact ctccggcgtc tgtttcggca
     8581 ggccgtaaat caggtcgatg ttggtggagg taaaaccaat ctcacgcgca tggttaagca
     8641 gtgcaaagat gaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga
     8701 agtcctgcac gcccatgctc aggcgattaa agccttcggc gcgtaaatga tcgagtacat
     8761 ccagttcgat ttcccgcgga tcgacttcga tcgaaatctc cgcatcggta ttgaaatgga
     8821 agttttcgcg cagcagcttc atcagacggc tgatttgcgc tttattcaga tacgtcggcg
     8881 ttccgccgcc ccagtgcaat tggctgacgt gacgcccggc gaacagcggt gcacgatgga
     8941 cgatttcttg ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa
     9001 tcttattgca accgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag
     9061 ataatggacg ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt
     9121 ctgaaaactc cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct
     9181 ggatcagggc cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg
     9241 tctctggcgg ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag
     9301 ccacttttgt cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat
     9361 aaccggaagg gttatcagca gaacgggaag gcccacggcg gcaatccgtt agttgccccg
     9421 cagcagacgc atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata
     9481 agagaggccg agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga
     9541 ttgctcttcg gcgctcaggg tttcgcctgc ttccagacgt tccagcagca catccagacg
     9601 ctcatccgtt tccagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt
     9661 cggtttgtgc tgtttggtga ctttttcagt cactcccaat ggaatagggg ttttactgcc
     9721 aatacgtgga tcttttggtg cgttctggcc tttgctgcct gacgtggtgt ttccgcccgc
     9781 tgcgcggctg cccggcgcat gaccacgacg ttttttctga cgcttacggt cacgagcttc
     9841 ctgatccagc tcctcgcgtg tttttcgacg cgcttttgca tgacctttgc tgcgtgagtt
     9901 tgaagatgat ggtttcataa tggttcgtct tttagccgtt ttattcagta tagatttgcg
     9961 gcggaatcta acagaaagca agcaaagaaa aaaggcgaca gattactctg tcgccttttt
    10021 tcctgactca taacccttaa cgggtcttac aatccttgca ggccgccaac acaccctgtt
    10081 tttccattag ccggaacaat ccgtgttgat tccatttccg tttaattacg tctccggacg
    10141 tttgtcttcc tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt
    10201 cattgttcct gttagcgtcc tcgctctatg ggtgctactt tacgcctttg ctttaaacaa
    10261 acaatcaacc gccatcagta tttgcacatt ttcagagttt tatataaaag caattcactg
    10321 atttatatag cttatttgac atgcatcgca gaattttctc tgctatttcg cataaagact
    10381 atcggcaatg tctcacaaag cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg
    10441 ccttttatta tggttcaggt ataatcgcca caaagctttg actgacggag acgaccgctt
    10501 tgactaattt gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat attcgccacc
    10561 taccttccga taccggaatt gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca
    10621 gcgcgctgaa cacgctgact aaccagaaaa gcctggctcg tacctcaaaa accccagggc
    10681 gcacccagct tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt gacttgcctg
    10741 ggtacggtta tgcggaagtc ccggaagaga tgaagcgcaa atggcagcgt gcgctcggcg
    10801 aatacctcga aaaacgtcag agcctgcaag gtctggtggt gctaatggat attcgccatc
    10861 cgctgaaaga tttggatcag cagatgattg agtgggcggt agacagcaat atcgccgttc
    10921 tggtgctgct gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg caattgaata
    10981 tggtgcgtga agctgtactg gcgtttaacg gtgatgtgca ggttgaaacg ttttcttcgt
    11041 tgaagaaaca aggcgtagac aagctgcggc agaaactgga tacctggttt agcgagatgc
    11101 agcctgtaga agaaacgcag gacggcgaat aattttcttg ccttaatgct tgtgccggat
    11161 gtggcgtatc cggcccgtaa attcaataga ttacaattct accgttggcc tgataaacat
    11221 agcgcatcag gcattacgga tcttttcttt cgcccaataa aaaacgcccc agtcattact
    11281 gactggggcg gctaaaatat tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga
    11341 tagaacatct tacctctgta ccctacgcga ctaactttac tcttttttgt tcagttcaga
    11401 aagcactttt tgtaattttt tttcagttgt tgcataggaa tttctaatag ccatcacaaa
    11461 acgtgacagc ttatgttgct tacttacgaa aaaagggcat gttcaggcga atcttagtgc
    11521 gcctgatccc agttttcgcc actccccact tccaccagca acggcacatc cagacgggta
    11581 cagttttcca tcagttgatg aatctgcttc gcgacggcat caacatcatc tttatgaact
    11641 tcaaatacca gttcatcgtg tacctgcatg atcatacgta cacgcggttg ctcagcctgt
    11701 aaccacgcat caacggcaat catcgcccgt ttgataatgt cggcggcggt tccctgcatt
    11761 ggcgcgttaa tggctgcacg ttcagccgct gcacgacgag caccattgct ggatttgata
    11821 tccggcagat acagacggcg tccgtccagc gtttcaacgt agccctgctc tttcgcctga
    11881 gcacgggtgc gttccatata ctccagcacg ccagggtagc gttcgaagta aaggtccatg
    11941 tacttctgcg cttctttacg tggaatgttc aattgccgcg ccagaccgaa agcactcatg
    12001 ccataaatca gaccaaagtt gatcgctttc gcgctacggc gttgctcgct ggtgacggtt
    12061 tccagtggca aaccaaacac ttctgccgcc gttgcccggt ggatatcttt tccttccgcg
    12121 aatgcggtca gcaagccttt atcacgcgag agatgcgcca taatgcgcag ttcaatctgc
    12181 gagtagtccg ctgagacaat cacataatcc tctggcgcaa taaacgcctg gcggatacga
    12241 cgaccttctt cgttacgcac cggaatgttt tgcaggttag gatcggttga cgataaacgt
    12301 cccgttgcag ttactgcctg gtgataagag gtatgcacac gcccggtttt cgggttgatc
    12361 atcagcggca gcttgtcggt gtaagtcgac ttcagcttcg ccagaccacg atactccaga
    12421 atcacttttg gcaacggata gtccagcgcc agttcttcca gtacctcttc cgacgttgac
    12481 ggcgcgccac ccggcgtttt cttcagcggt ttaataccct gtttttcaaa cagaatggtt
    12541 tgcaactgct tggtggaaga aaggttaaat tcttcacctg caatttcatg cgctttcttt
    12601 tccagctcag ccagacgcaa ggtgagctct tcagaatgat tgtgcagcac tttcggatcg
    12661 atcttcacac cgttacgttc aatgcgtgaa agcaccggca ccagcggcat ttcgatattc
    12721 tcgaagacgt tcaacggccc tttgtgtttt tgcagatccg gccacatttt cagatgcaac
    12781 tgcaaggtga catctgcatc ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg
    12841 ttaaaggtca gttgattttt gcctttaccc gcaatctctt caaaagtgat ggttttgtgc
    12901 ttcaaccaac gttccgcgag gctgtccata tcgtgacgcc cggcaacgct attgagaatg
    12961 taggactcca gcatggtatc aaacgcaatc ccacgcagtt caatgccgta attcgccaga
    13021 ataccgcgat cgtatttcag gttttgcccg accttcagcg ccttttcatc ttccagcagc
    13081 ggttttagca actcgagtgc acgctcgtga gagatttgat cgggcgcatc aagataatca
    13141 tgagcaaccg gaatatatgc cgctacgcct ggctcgatag caaaagaaag cccgaccagg
    13201 ttagcagaga tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt
    13261 tccagcttcg caatccacgc tttcagtgtt tcttcatcaa ggatagtgac gtagttgtca
    13321 taagaaatca ccgttgccgt cacttctggc gcttcgtctg caacactggt ttcctgcggc
    13381 ttcgcggctg gttttgcccc tttggcctgt aaccatttgc ccgcttcgac atcagcagtc
    13441 cagcgtttga actcatactt tttgaacagc cccaacaact cttccgctgc cggttgctgc
    13501 acttccagtt gttcacaggt cagctccagt tcaacatcgg ttttaatcgt cgccaactgg
    13561 tatgagagat aagcaacttc tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca
    13621 cggaagctca acccagcaat tttttctggc tcggcataca gcgtatccag cccgccaaga
    13681 ccttgcagca atgcctgcgc ggttttttca ccgacgcccg gtacgccagg aatgttatca
    13741 gaggagtcac ccatcagcgc caggaagtcg atgatcaatt ctggcggcac gccgtactta
    13801 ttcaccacct cttccggtcc gaggatggta ttcgtcatgg tattgataag cgtaatattt
    13861 ggcgtcacca gctgcgccat atctttatcg ccagtgctaa tcagcaccgg acgcccggct
    13921 ttttcggctt cgcgcgccag agtaccgata acgtcgtccg cttctacgcc agaaaccgcc
    13981 agcagcggca gtcccatcgc tttaaccatc gcgtgcagag gttcgatttg tgctcgcaga
    14041 tcgtccggca ttggcgggcg atgtgatttg taatgttcaa acagttcatc acgaaaggtt
    14101 tttcccttgg cgtcaaagac caccgctgca tgcgtcggtt tatattgcat gatcagactg
    14161 cgcagcatgt tgaggacacc atacatcgca ccggtcggct cgcctgcgct gttagtcagc
    14221 gggggaaacg cgtgatatgc gcgataaaga taagatgaac catctacaag gataagtgga
    14281 ttttggggga tctgaaccat aatgtccgtg cctgtttatc agattatggg taaaggatgc
    14341 cacagacagg atgaaaacat gagtttttca ccgcttttcg tgaaaatctt ttgtagatct
    14401 gctggatcgc tcgcaaatcg tcatgtggat aactttgtgc ataaattatt tacatcgctt
    14461 ttaatttatt atcatgcttt ttacagcatg atgtttgtct ttgtttttca tgcagttaaa
    14521 aataaatcca tccctaataa ttgagataat gggatttttt ctaatgtgga tagatatgaa
    14581 ttatttttct ccttaaggat catccgttat ttgggtcatt ttctgctaaa atctgcgcct
    14641 tgcgcttctt tagtgcactg tctatggcta accttttgaa taaattcatt atgacgagaa
    14701 tactcgctgc gataaccctt ttgctgagta tcgtattgac cattttggtc actatttttt
    14761 gttccgtccc gatcatcatt gccgggattg taaaactatt gctacctgtg ccagtcattt
    14821 ggcgaaaggt ttcgcgtttt tgtgatttta tgatgtattg ctggtgtgaa ggtctggcgg
    14881 tattactgca tctgaaccca cacctgcaat gggaagttca cgggctggaa gggttaagta
    14941 agaagaactg gtatctgctt atttgtaacc accgtagttg ggcagatatt gtcgtactgt
    15001 gcgtgctgtt tcgtaagcac attccgatga ataaatattt cctcaagcaa cagctggcct
    15061 gggtgccatt tcttggcctg gcgtgctggg cgctggatat gccgtttatg aagcgttatt
    15121 cccgcgctta tttattacgt catccggagc gacgtggtaa agatgttgaa accactcggc
    15181 gttcttgtga aaagtttcgc ctgcatccca ccaccattgt caatttcgtt gaaggctccc
    15241 gtttcacgca agaaaaacat cagcaaaccc actccacttt tcaaaacttg ttgccaccaa
    15301 aggcggcagg cattgcgatg gcgctaaatg tactgggtaa acaattcgat aaactgctta
    15361 atgttacgct gtgttatccg gacaataacc gtcagccgtt cttcgatatg ttaagcggta
    15421 aactgacgcg gattgtcgta catgtggatt tacagcctat tgccgatgag ttacatggcg
    15481 actacatcaa tgacaaaagc tttaagcgcc attttcagca atggctgaac tcattgtggc
    15541 aggaaaaaga ccggcttctg acgtctctca tgtcatcaca gcgtcagaat aagtaagttt
    15601 tactcgcctc tgaatgagca gaggcgagtg agtattttaa tgaacaaaac gtccggcacg
    15661 agacataaat tcttcttcgc tcatctcctg tccgttaaaa acaactttac ccggtgtata
    15721 acgcagctgc agcgaggcgg tattgttgtc aatcgtgatt aactgaaaca tctgacctaa
    15781 tgtcatcatc ccgctgattt gtttatcagc ctgcttttgc gctttttccg catccattcc
    15841 ttcagataaa ttaagctgtt ttgcggtttc tgtgacgaca ttaagcggta acttcacatc
    15901 aaaattgagc gatttgatat ctttgtttgt ggatgatgaa gatttggctg ggtcggcaat
    15961 actgatatcc agattggcat ttagttcgcc gagtgcgttc ttccatctta ccggttgttt
    16021 aatggtcggc tcactttttt gtaataacgg taaatactct ttgaacaatg cagcattcac
    16081 ttcttgcaga gcaacttcat cgtttgctaa ctcagggtgt gcagcaagct gcttctgcat
    16141 cgcaatgtta tactggatga taaattggcg cactgctgag ggatcaatag attcagcaat
    16201 caatgaccac tcaccactac cgagttgctg attccctttt gttaacttat caagttcgta
    16261 ggtcagattg acattgatat aatctttgtc tttaacgcga tccagcctgt tttttgcaac
    16321 gaaagcatcg atttgggcaa cgtcttcccc gtgattgata atgttaatct gattaaattt
    16381 cgactcgctt tcaccaaccg gaaatttctt ttcttctaat cgcgccagag aatcaatggt
    16441 aaaacttttt gccgttactt tgttcattgg tgataattgc agaacaatat cttcgacttt
    16501 gccttcaata tccaggttag acaatgaact accatcacca ttaaaagtaa actggccctc
    16561 accaaaagaa aactttccat actctgcagg ggcaacatct aatgtcgtag agaatgagcc
    16621 accaaacgcg aatcgcaatg tcgcttccac gggcgattta tttttcgcgg cgataaatag
    16681 cggttgtgtc agttcattat tcactaagtt cacttttgcc gctgccagtg ctgggatgac
    16741 attaccatgc attaacattg tgatgggcaa cggaccgtgc tcaacatcca catcaaatac
    16801 aactttctgg cctggcttga tattgagatc gggtgcatcg ttatcgaatg tcatttgcat
    16861 ttgaaaacgg gaattaaaaa agcctttgtc aaagttttta taacttaacg tcatatcgta
    16921 ggcatgttca cctttctttt tcgcatcgtt aaaatcttta ataaactttt cgataccagg
    16981 ctgaatttgc gtacctgtgt accatgtgcc accaccccag attacggcta acgcaacaat
    17041 aacacctgta gctgacttac gtatcatata aatattccct gataaaacag caaaactttg
    17101 aatatccact tatgctgaaa taatgtccag cggcaggatg cattatcaga atgtatttat
    17161 atttattaaa taataaaaaa agcccgtgaa tattcacggg ctttatgtaa tttacattga
    17221 attatttttt ctcggacaga tatttcaccg tatcagcata ctgctgaaca aaaacatcca
    17281 tattgctggt atccataccc tgcggattca gctgatattt accgttaaca aacatcgccg
    17341 gaacaccacg caattgcaca tcagctgcgg ctttttcctg ctgagcgacc agagatttca
    17401 ccacgaagct gttccacgcc gcgtcgtact cttcaccttt aatacctgca ttgataaata
    17461 catcgcggat atcagatgct gaacgaatgg tctgggtttt ctgtacgcct tcaaacagcg
    17521 gaacagtcac tttgtcttcc acgcccagcg ccatcgccac agcccatgcc tgagtcagat
    17581 ctttgcccag gtcaccaccc atgaagttga cgtggtattt agtcatcttc acgccttccg
    17641 gcagtttttt cttcacatta tcagaaatat gcagaacttc ttcaaactga tagcagtgcg
    17701 ggcagaagaa agagaaaaac tccagcactt gcggcgcgcc agctaccggt ttttccaggg
    17761 tagtgtactg tttaccatct tcatactgcg ccgccgatgc gctaaacgct aaaactaaac
    17821 cagccagcgc cagccaaatc tttttcatga tctactctct ccgattaata cataggtgtt
    17881 aattgcaaag ggggttcttg tagaactttt gcctgttcta taaaagtcgc cgtctgtcgt
    17941 agccagtaat cttccccggt taaccacggg aaatttttcg ggaacgcggg atcagcccaa
    18001 tgccgcatta gccaggcaag ataataaacc aaacgcatgg cgcgtaaagg ttcaatcagt
    18061 ccgatttcag cggtgtcgaa ctcgctaaat tcttcataag cttcaataat agtttccagt
    18121 tgcatccgct gctcggcttt atcgccattg agcaacatcc acaaatcctg aatagctgga
    18181 ccattacgtg catcatccag atcaacaaac attggaccat cgcgccagag aatattcccg
    18241 gcgtggcagt ctccatgtag ccgcagaacg gtgaaatctt cccgccagtg tgctgtaacg
    18301 gcggcgatca gctcatctgt cgctttcaga aatgccgctt tcaacccgga aggtatcagt
    18361 gtagcgtcct caaacagctt gcgaggctca atgagatatt cattcagacc gatggtcggg
    18421 cgatgaataa aaagctgttt gcgccccgtc tggtgcatac gccctaaata acgcccaacc
    18481 gcttccatct gatcgatatt atcagcttcg aactggcgac caccgacgct tggaaaaaca
    18541 gcgaaataaa atccctgatg attcaataaa gtctgaccgt taaaggccac aggtgctgcg
    18601 accggaactt catcatttac cagctgcaac gcaaattgat gttcttcgag gatttgatcg
    18661 gctgtccaac gttcagggcg ataaaatttg acgacaaaac gtcgacgctc ttcgtcctga
    18721 aattgataga cacggttttc atagctgtta agcggggtaa gaccggaatc cacccggatc
    18781 ccttgctcaa acagagcgtc catgatggta tccgggtgta gtgtctggaa agtaaaagcg
    18841 ctgttgttca tcccatcatc cggaaaacat tacgaatgat gaaggatatc atttcacgat
    18901 gattttggtg gcgccgctta caagctttta ctctttaatt acgccgcgag cgcgtaacag
    18961 cgccgttttg aagtcttctt cataatcttt ctgcaaaccc gggatcaccg catctttcgc
    19021 ggaatcacgc attttcaggt gatagatcag aatgtcatcc gtcaaatccg ccagttcgcc
    19081 gtcaaaacct gactctttcg ccagtttctg caaaaattgc aacaagttaa agtctggctc
    19141 tttttgccag gctggctgga ggagttcaat aacttcattc agacgtttac atttcatagt
    19201 aatgctcctt actcttgaga cagacacgtt agcagggtca atcccacaat aaaagaggcg
    19261 atatcggtga atctgatgac gacgataaca ggcgttgtgc tggcaggcgg taaagccaga
    19321 cgaatgggcg gcgtagataa aggattgctg gacttaaatg gcaaaccgct gtggcaacat
    19381 gtcgctgacg cgcttaagac gcagctctct cacgtcgtgg ttaatgctaa tcgtcatcag
    19441 gaaatctatc aggcaggcgg tctgaaagtg attgaagatt cactggcgga ttacccaggc
    19501 cctctggcag gaatgctttc agtaatgcag caggaagctg gtgagtggtt tttgttttgt
    19561 ccgtgcgata cgccttacat tccccatgat ttagccgccc ggcttaatca tcagcgcaaa
    19621 gatgcgcctg tcgtgtgggt ccatgacggt gaacgcgatc acccgactat tgctctggta
    19681 aaccgcgcta ttgagccttt attactggaa tatctgcaag caggagaacg ccgggtaatg
    19741 gcatttatgc gtctggctgg cggtcatgcg gttgatttca gcgatcataa agatgcattt
    19801 gttaacgtga atacgccaga ggagcttgcc cgatggcagg aaaaacgatg ataccgttac
    19861 tcgcctttgc cgcgtggagt ggtaccggaa aaactacgct tttgaaaaaa ctgatcccgg
    19921 cattatgcgc cagagggatc cgtccggggc tgattaagca tacgcaccat gatatggatg
    19981 ttgataagcc aggcaaagat agctatgagc tgcgcaaggc tggcgcggca caaaccatcg
    20041 ttgccagcca gcaacgatgg gccttgatga cggaaacacc agacgaagaa gagctggatc
    20101 tacattttct cgcaagtcga atggatacct caaagctgga tttgattctg gtcgaagggt
    20161 ttaagcatga agagatcgca aagattgtgc tgtttcgcga tggagccgga catcgacctg
    20221 aagaattagt gatagacagg catgttattg ctgtagccag tgatgtgccg cttaatcttg
    20281 atgtcgcatt actggatatt aatgatgttg aggggctggc tgattttgtg gtggagtgga
    20341 tgcaaaagca gaacggataa gcaagagtag gctggatcag acgcagccag tgtcgcatcc
    20401 gacctttttc tgcaccagcg caaaaaggcc cgactgggcc t
//
LOCUS       DBKEKI010000062        20440 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.062, whole genome shotgun sequence.
ACCESSION   DBKEKI010000062 DBKEKI010000000
VERSION     DBKEKI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.062"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(28..879)
                     /gene="manZ"
                     /locus_tag="ACUQPX_002307"
     CDS             complement(28..879)
                     /gene="manZ"
                     /locus_tag="ACUQPX_002307"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HHV9057006.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(892..1692)
                     /gene="manY"
                     /locus_tag="ACUQPX_002308"
     CDS             complement(892..1692)
                     /gene="manY"
                     /locus_tag="ACUQPX_002308"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HHV9057007.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(1755..2726)
                     /gene="manX"
                     /locus_tag="ACUQPX_002309"
     CDS             complement(1755..2726)
                     /gene="manX"
                     /locus_tag="ACUQPX_002309"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HHV9057008.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPAEKAAPAPAAAAPKAAPTPAKPMWPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3043..3201
                     /locus_tag="ACUQPX_002310"
     CDS             3043..3201
                     /locus_tag="ACUQPX_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HHV9057009.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            3189..4739
                     /gene="yoaE"
                     /locus_tag="ACUQPX_002311"
     CDS             3189..4739
                     /gene="yoaE"
                     /locus_tag="ACUQPX_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HHV9057010.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(4743..6341)
                     /locus_tag="ACUQPX_002312"
     CDS             complement(4743..6341)
                     /locus_tag="ACUQPX_002312"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HHV9057011.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LVQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6472..7836)
                     /gene="sdaA"
                     /locus_tag="ACUQPX_002313"
     CDS             complement(6472..7836)
                     /gene="sdaA"
                     /locus_tag="ACUQPX_002313"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HHV9057012.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDIYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8020..8598)
                     /locus_tag="ACUQPX_002314"
     CDS             complement(8020..8598)
                     /locus_tag="ACUQPX_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HHV9057013.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(8602..9963)
                     /gene="pabB"
                     /locus_tag="ACUQPX_002315"
     CDS             complement(8602..9963)
                     /gene="pabB"
                     /locus_tag="ACUQPX_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HHV9057014.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10037..10216
                     /locus_tag="ACUQPX_002316"
     CDS             10037..10216
                     /locus_tag="ACUQPX_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HHV9057015.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(10336..10635)
                     /locus_tag="ACUQPX_002317"
     CDS             complement(10336..10635)
                     /locus_tag="ACUQPX_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HHV9057016.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(11057..11401)
                     /locus_tag="ACUQPX_002318"
     CDS             complement(11057..11401)
                     /locus_tag="ACUQPX_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HHV9057017.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11533..13443
                     /locus_tag="ACUQPX_002319"
     CDS             11533..13443
                     /locus_tag="ACUQPX_002319"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HHV9057018.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQRALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13501..14196
                     /gene="tsaB"
                     /locus_tag="ACUQPX_002320"
     CDS             13501..14196
                     /gene="tsaB"
                     /locus_tag="ACUQPX_002320"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HHV9057019.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14236..14817
                     /gene="yeaY"
                     /locus_tag="ACUQPX_002321"
     CDS             14236..14817
                     /gene="yeaY"
                     /locus_tag="ACUQPX_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HHV9057020.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTLQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15022..16707
                     /gene="fadD"
                     /locus_tag="ACUQPX_002322"
     CDS             15022..16707
                     /gene="fadD"
                     /locus_tag="ACUQPX_002322"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HHV9057021.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRTGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            16789..17904
                     /gene="rnd"
                     /locus_tag="ACUQPX_002323"
     CDS             16789..17904
                     /gene="rnd"
                     /locus_tag="ACUQPX_002323"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HHV9057022.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17958..18923)
                     /gene="yeaX"
                     /locus_tag="ACUQPX_002324"
     CDS             complement(17958..18923)
                     /gene="yeaX"
                     /locus_tag="ACUQPX_002324"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HHV9057023.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(18979..19608)
                     /gene="yeaW"
                     /locus_tag="ACUQPX_002325"
                     /pseudo
     CDS             complement(18979..19608)
                     /gene="yeaW"
                     /locus_tag="ACUQPX_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(19938..>20440)
                     /locus_tag="ACUQPX_002326"
     CDS             complement(19938..>20440)
                     /locus_tag="ACUQPX_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9057024.1"
                     /translation="AFAAKCGLSDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQ
                     SQSMPMVEHKNTQENKNPQPDRSTLKTKLIAIVELLEDKELHSAIELFRKKGFEALMP
                     EIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKEILSKYGIYEQEGLVAPSQEPQ
                     DVKKAV"
BASE COUNT         5020 a         5110 c         5278 g         5032 t
ORIGIN      
        1 aaaaggcccc ggtagtgtac aacagtctta cagtcccagc aggccgcaag cgtaaccagc
       61 gataccgatg acgaagaagc caacgatgat ccacagcggg ttaacttttt tgcgcagcag
      121 ccacatacaa gcaaatgtca gcagcagtgg tacaaggcct ggcattaact ggtcaagaat
      181 agtttgcacg gtggtgacac gggtctgtcc atcctgcccg gtgatggtcg aaaccaccag
      241 cgggatgttg acatgtgtcc acttgttaac caatgccccc atgacaaaca ggccgaggat
      301 agacgcccct tccgtcagtt tttgcaggaa gccaccaccc atatctttaa cgatatcgat
      361 acctttggag taaccatacg ctacgccgta gtaacgggtt gccagacgca ccaggttaaa
      421 caggatgaag aacagcagcg gacctaacag gctgccgctc atcgcgatac cggcacccag
      481 tgctgcaaat accggacgta cggttcccca gaagatcggg tcgcctacac cagccagcgg
      541 ccccatcaaa ccgactttga taccgttgat agcaccgtca tcgatctctg cgccattagc
      601 acgctgttct tccagcgcca gggttacgcc gagaatcggt gcagccacga acggctgggt
      661 gttaaagaac tccaggtgac ggcgaatagc ttgtttacga gcttcgttgt tctcagggta
      721 gaggcgacga attgccggta ccatagagaa gcagaaaccc agtgcctgca tacgttcgaa
      781 gttccatgaa ccctggaaga ggttagaacg caggaatacg ccacgaatat cactttgagt
      841 gagttttttc tcggtggtag tttgagttgt atcaaccatt tcgctcacct gttagtccag
      901 ttcgttatcg agatcgttgt taccagctgc ctgagcaggc gcaccggcta cgcggttgta
      961 tttcgggcta agttggatgt agagcactgc cataacagta ccaatcacac ccagagcaac
     1021 caggttaaag ttggtgaatg ctgcggttac gaagccgagg tagaagaacg gcatcaggta
     1081 gccagcacgc atcatgttga taaccatcgc ataaccaacc accacgatca tgcccccggc
     1141 gatattcaga ccattggtca ccacttccgg aatcgcattc agcatgttct gtacttcgct
     1201 ggtaccaaca gacagcgcaa cgatgacggc cggaatagct acacgcattg cttgcaggaa
     1261 cagagaagaa acgtggatcc aggaaatcgc tgtcaggttg ccgttatcag cagccttatc
     1321 cgcagcgtgc tggaaagcaa cggtaatagt acgaacgatg atggtcagta cctggcccgc
     1381 agcggccaga gggattgcca gtgcgatacc tgcaccaatg ctctgatgac ctgcgataac
     1441 cagaatggta gaaatgatag aagccagagc ggcgtcaggc gcaactgcag caccgatgtt
     1501 catccagccc agcgcgatca tttccagcgt accaccgata ataataccgg ttttcatatc
     1561 cccaagaacg atacccacca gggtacacgc gattagcgga cggtgaaact gaaattcatc
     1621 gaggattgat cccatacctg cgatacaggc tacgataaat accagcacaa tttgaagagt
     1681 ggtaatctcc attgtacttc tcctgtttac gtaagactta agtgtgaaaa ctgaatgata
     1741 atcaacacaa tacgttactt atcgattttg ctgatcagat ccatcatttt cagtttcgga
     1801 tcggtggaaa ccttacggac ttccagctca ataccgcgtg cattcagttt tttgaacgcc
     1861 tcgatatctt tttcatcaac cgaaaccgcg ttattcacct gggttttacc ctgacggaat
     1921 gccataccac cgacgttaac agaggtgatt ttcacgccgc cttcaacgag acgctctaca
     1981 tctgttgggt tggtaaataa cagcattacg cgttcgccag catatttcgg gttgttgtag
     2041 acgcgaatca ttttggcaac atcaactacg tgtgctgtta cgcccggagg tgcaacctgg
     2101 gtgagcagtg tcttacgaac ggtatccgca gccacttcat cactaacaac aataatacgg
     2161 gagacattgg tttctttggt ccagcgggtg gcgacctgac cgtgaatcag acggtcgtcg
     2221 atacgcgcaa ggccaataac catgtagtcg tttggccaca ttggttttgc cggagttgga
     2281 gccgcttttg gtgctgcggc agcgggtgct ggcgcggctt tttcagccgg tttggctttc
     2341 agtgctttca cgccttcacg gcctgtttct actgccagcg caaccagttc atcaaagctc
     2401 gggtcatcat cacgggccat taacgtttcc acgagcattg gaatgttaac gcctgcaatg
     2461 acttcataat gctctttgtc gacgacaatg cggctggcag cattgaacgg gctgcctccc
     2521 catgtatcaa cgagaaacag cacgccttta gtggtgtcga gttttgccaa ctgagcgttg
     2581 tacttttcaa tcagcgtttc ggcattttca cctggaacga aatcgatcca gccgacgttt
     2641 tcctgctcgc ctaacagcat ttctgccgtt ttaagcaact gctctgcagc ccaaccatgt
     2701 gtgcctataa caatagcaat ggtcacttgc tacctccttt attatcgtta acacctcaac
     2761 gtgccagatg tatttttgaa tcgtcgtcca caatcgaatc gattcagata agggctacaa
     2821 ccaaaaaccc ctgcgtttcg cgatttattt tagatatcga aaaaattatt ttatgtgatg
     2881 aagatccgta atttaacttt cgattatcag aaatttcgaa aggtaaaata tccttgtcac
     2941 attcgtttgc aaaggaaggt aaatctttgc caaatcagag gcgtctctga tatgtttaac
     3001 tcccgtttaa caaccatgga gtatagggca gtagcccgca gtatggatcg tcaccgacgt
     3061 catttcagca tcaggccttt taacgcctgc ctttctggca ctctatgccg cacctttcgt
     3121 ttgcattttg tcgttacgcc tgcattattt ctggcgtcga atagctattc cttaagcagg
     3181 agcttgtcat ggaattctta atggacccct caatttgggc ggggctactc acgcttgttg
     3241 ttctcgaaat tgtgctgggt atcgataacc tggtcttcat cgccattctt gctgacaaac
     3301 tgccgccaaa acaacgcgat aaagcgcgtt tgctggggtt atcactggcg ctgattatgc
     3361 gtctggggct gctgtcgctg atttcatgga tggtcacgct gaccaaaccg ctatttaccg
     3421 tcatggattt ctccttctcc ggacgcgatc tgattatgtt gttcgggggg atattcttgc
     3481 tgttcaaagc aacaaccgaa ctgcatgaac ggctggaaaa ccgcgatcat gattccggcc
     3541 acggtaaagg ctacgccagt ttctgggtgg tcgtcacaca gattgtcatc cttgacgccg
     3601 tcttctcgtt ggatgcggta attactgcag tagggatggt taaccatctg ccggtgatga
     3661 tggcggcggt agtgattgcg atggcggtta tgttgctggc atcgaaaccg ctgacgcgat
     3721 tcgttaacca gcaccccacg gtggtggtgc tctgtctgag cttcctgtta atgattggtc
     3781 tgagtctggt ggcagaaggt ttcggtttcc acattccgaa aggttacctg tatgccgcga
     3841 ttggcttctc gatcattatc gaagtgttta accagattgc gcgtcgcaac tttattcgtc
     3901 accagtcgac tttgccgctg cgagcgcgta ctgccgatgc catcctgcgt ttgatgggcg
     3961 ggaaacgtca ggccaatgtt cagcacgatg ccgataaccc gatgccgatc ccggaaggtg
     4021 catttgccga agaagaacgg tacatgatta acggcgtact gacgctggcg tcgcgttctc
     4081 tgcgcgggat catgacgccg cgcggtgaaa taagctgggt tgacgctaat ctcggggtcg
     4141 atgaaatccg cgagcaactg ctctcttcac cgcacagtct gttcccggta tgtcgcggtg
     4201 aactggatga aatcatcggt atcgtacgtg ctaaagaact gctggtggcg ctggaagagg
     4261 gcgttgatgt ggcggcgatt gcttcggcgt ctccggcgat tatcgtcccg gaaaccctcg
     4321 atccgatcaa cctgctgggc gtgctgcgtc gcgctcgcgg gagctttgtt atcgtgacca
     4381 acgagtttgg tgtggtacaa ggcttggtca cgccgctgga tgtgctggaa gccattgcgg
     4441 gtgaattccc ggacgctgac gaaacgccgg aaatcattac cgatggtaac ggctggctgg
     4501 taaaaggcgg tacagatttg catgccttgc agcaggcgct tgatgttgag caccttgccg
     4561 atgacgatga tatcgcgacg gtcgcgggcc tcgtgatctc ggcaaatggt cacattcccc
     4621 gtgtgggcga tgtgattgat gtagggccac tgcatatcac catccttgaa gccaatgatt
     4681 atcgtgttga tctggttcgc attgttaaag agcaaccggc gcacgatgaa gatgagtaag
     4741 cgttaacgta acgacataat gggcgtgata tgtccattat gccgggcggg cggcggttgg
     4801 ctgcccgcca gccatttggg aaaatcacgt agcggcatcg gctgtgcgta caaataccct
     4861 tgcaaaatat gcacgccatg acggcgcaaa tacttcgcct gttcttgcgt ttccacgccc
     4921 tccgccacca gttcaatatt cagtctttgc cccagcgcga tgatgatatc ggtcaccgtc
     4981 gagttaaccg cgtcagaacc tatagctgcg gtaaatgact tatcaatttt cagcacgtca
     5041 ggacgtaatg tttcaagcca ggaaaacgag ctgttgccgg tgccgaagtc atcaatcgcc
     5101 agtttgacgt ttttacgatg cagctcgcgg gcaatccgat aatcaacatc cagtaaggca
     5161 tcgcgttcgg tgatttcgag gatcagttgc tgaatcgggt gagcgctaaa ccagtactga
     5221 ttgagatctt ttatcagcac accacggcga aaatggctgg gtgcgacgtt aatgccaaca
     5281 tgaaactgac tactcatcgg gaaaacatgg cgctgacgaa tggtttctgc catcacatag
     5341 cgggtcagtg gcacaattaa atgatgttct tccgcgatag gaataaacac atccggtgaa
     5401 atccagccct gacgcggatt attccagcgc agcaaaatct ctacaccaat acattgctgg
     5461 ctgcgcgcat taagcaaagg ctgacagaac aattcgaact catgttgcac caggcccaga
     5521 ttaatttcgc gggaaaagct catccggtaa gcggtcgcca gccaggcgat atagcccacc
     5581 agtaaactta gcagcaccgc cagcggtaat tgtgttggaa gatagtgcca tgccagcgcc
     5641 gtagcccccg gaccatttac gttaatggta aaaggaaagc tttgcgaaga aacctgatag
     5701 ttttcattgt cttcaggttg cggaagggaa tctaccagcc cattaccata gagcaaatgc
     5761 cgtttgtcca ccgtcaggct ggcgctactg atttgcggca gttgtggctc aaggagcatt
     5821 gccgtcagta agtcgatgtt aatcatctcc atgaccccag cattgctact gcccgctgca
     5881 ggcgtccatt gaatcaaaac cggactgcct ttaatcaggg cacggtcgat cgtcaggcgt
     5941 aaaagtggtt gcggtgcagg aagttcagcc agaatgtcca cgacgggcac attgcgataa
     6001 ccaaaaatgc tggagcaata aagtgtgccg tcttgcacca ggccaatgga tcgcacagtt
     6061 tggagttttg ccgcctgttt acgtaatggc aaatgggcga cagagcaggg cagaccaatc
     6121 agcggaagca agacttcgct acctgcctgt aggggaagca gtactttatc taattcctct
     6181 acagcgtgat tggcgaattg tactacccgt tgttgattta agttacgctg tgaaataaat
     6241 cgcacgctca gggtcgaagc gagcgtaacg agtgcgcaaa tcgtacaaac aatcattcga
     6301 ttacggcgat aggttttaat gatccgttgt gctttttgca tgcgggccac cctgtaagcg
     6361 tatggcgaca aacgcctgac gcaacagtgg aagtgtagtg gggaatatta cagcagacga
     6421 gaaagcgggt ataaattcgc ccatccgttg cagatgggcg agtaagaagt attagtcaca
     6481 ctggactttg attgccagac caccgcgtga ggtttcgcgg tatttggcgt tcatgtcctt
     6541 accggtttcg tacatcgttt cgatgacctt atccagcgag acgcgcggtg cactggtgcg
     6601 gcgcagagcc atccgtgcgg cgttaatcgc cttcacagag gcaatggcat tacgctcaat
     6661 gcacggcacc tgaacctgcc ctgcaaccgg gtcgcaggtt aaaccaaggt tgtgttccat
     6721 gccaatttcc gccgccacgc aaacctgttc cgggctaccg cccagcagtt ctgcaagacc
     6781 cgcagcagcc attgaacagg caacacccac ttcgccctgg caaccaactt ccgcaccgga
     6841 aatagaggcg ttcattttat acaatgcacc aatcgcgccc gctgccataa agtaacgggt
     6901 atagatgtct gggctgaccg attcaataaa gtggtcatag taagccagca ctgccggaac
     6961 gataccgcag gcaccgttgg ttggcgcagt taccacacga ccaccggcgg cgttttcttc
     7021 gttaactgcc agcgcaaaca tgtttaccca gtcaatgaca ttcatcggat cgttagacag
     7081 tttatcgctg gaaactaaca tccggcgcag ggcagacgca cgacgtggca cgcgcagcgg
     7141 gcctggcagt acaccttcgg tgttcatccc gcgatcgata catgcctgca tggtttgcca
     7201 gacatgcgcg aaatactcgt cgatctcttt cttgctgtgc agcgccagtt cgttctgcat
     7261 agcgagacca gacagcgaat agccggtttc attacagtac gcgagcagtt cggtggcaga
     7321 tttgaacgga tacggcacgc tcacttcgtt ggcagcatcc tgaccaaagt gttcttcatc
     7381 gacgataaaa ccgccgccga tggaataata agttttgctg tagacgactt catcgccgtt
     7441 ataggcgtgg atttgcatac cgttttcatg cagcggcagg ttgccgttat gaaaacgcat
     7501 cccgttgtcg cgcgggaaat ccacttcatg ccgtccctgt gccagcagca gacgttcgcg
     7561 ctcttttacg tcgcgaataa aaccgggaat actgtcgata tccacggtgg caggttcgtt
     7621 acctgcaaga cccataataa tggcgatatc ggtgtggtgg cctttacccg tcagcgacag
     7681 tgaaccataa atgtccacgg caacgcgagt aacgctatcc agtaagcctt tttcgaccag
     7741 atcatcgacg aactgtttac ccgccttcat aggccctacg gtatgggaag atgagggacc
     7801 aatccccacc ttaaacatgt cgaatagact aatcacgata atactcctga caaggtgact
     7861 ggacttccag taacgaacta ataacagcgc atagtgtaag agggaacgcg gcgactggct
     7921 taactattca catgaattaa actaatcaaa aaccgcgatt taactaatat tgcgatgatt
     7981 gtctcaagta gtgttttgta gatgtaaaga taagtatagt caggggttca caccaatttg
     8041 cagcgccagc tcacgaatta tgcctgcggt cattccccat acaaaatact gttcgtacca
     8101 ggacagccat acccgatgtg aatcgccacg gcggtagata tctaaagggt gataacgacc
     8161 cagatgtaat gcctgggcga gcggcatttc aaacaccgcc gagacttcat cttcactggc
     8221 gcgatacggc agatcgggcg ggataatgcc gaccactggg gttacctgat agccagttac
     8281 gctatcgacg ggcggcagca cgccgataac ttcaacggcg gaaggcggta tagcgacctc
     8341 ttcttcagct tcgcgcagcg cggcggcgat aactgatgcg tccgtgtcat cgactgcacc
     8401 tccagggaat gccacttgtc cagcgtgttt acgcagatga attgaacgct gagtcagcaa
     8461 caaccccggt tgcggtcgac ggacgatggg gattaacacg gcagcctgac gatgatttag
     8521 ggtttcccgg ttaatttgcg ggcgcaaaag ttgaaagcga gataaaaaat catcaagcgt
     8581 caggctacgg tattccacgt cttacttctc cagttggcgt aatatcttat taactttatc
     8641 aaaagtttcc tgatattccg cttcttcctg gctatcggcg acaattccac cgcccgcaga
     8701 gcagtatatt tgtccgttaa tggcagtcag cgtgcggata gtaatgctgg tatccatgtt
     8761 gccgcaaaag ctcaaatagc caatgctgcc gcaccaggca ttacgtcgct gcggttccag
     8821 ttcgtcgata atttccatag cccgtacttt cggagccccg gttattgagc caccaggaaa
     8881 agctgcgcgc agcagatcgc tggcgtgtaa ctgttctggt agtcgcgccg ttatagtgct
     8941 gaccagatga tgcacggcag ggaagggttc caccacgaag agctctggta cttttaccga
     9001 accggctacg gcaacacgac cgatatcatt acgcattaaa tcgacaatca tcagattttc
     9061 ggcacgatct ttcgctgagt tcgccagttt ttctgcttgt ttgctatctt cctgaggatc
     9121 gggcaggcgt ggtagcgtgc ctttaatcgg gcgggtctgg atttcactat tatcacaaag
     9181 aataaaccgc tctggcgaaa ggcttaaaat tgcaccctgt tcaagacgta aaaaagcgct
     9241 aaatggcgcg cggttggcct gattaagctg aaggaatgcc tgccattcat cgccagaata
     9301 ggtcgcatga aaacgctggg cgagattcac ctgatagcaa tcaccgctgt gcagatattc
     9361 ctgtacctgg cgaaattttt cgccgtactg ctcgcgggtc atattggatt gccagtcgga
     9421 agtgagcgtg aaatcttcct gcggcgagaa ttgctggctt tccagccagg cccgacgggc
     9481 attgacatca ttatgactca gcaaagaaac tgtatgacgc tggtggtcga caatgagcgc
     9541 ccaatcgtag atacccactg ccatatccgg cagaacgata tcttgttccg caatttctgg
     9601 cagtgactca aaacggcggc ccagatcgta gccaaacaac cccagtgcgc cgccctgaaa
     9661 tggcaaatct tcgttatgcg ttgggcgaat gtctgcgcga tccagcacct gctggagcac
     9721 ctgtagcggg tcatcagtgg tcgttgtgcg tttttcgctt tcactaacaa cggtttcttt
     9781 acctaaagtg gttaaagtgc aaatcggatc ggcgaccaca atatcaaagc ggctatacgg
     9841 atgatcggca tagccggagt gtaaaagcat cgcccacggc aggtggctta agcgggagaa
     9901 ataaaattca gcggcgtcct gacgccaggg taaagtaatc acagcgggag ataacgtctt
     9961 catcagtcct gactctactg gctatgtgcc gggtagcatg aaataattag cgccaacaat
    10021 ttagcaggag ttaacaatgt ttgcaggttt accttcactc acccatgaac agcagcaaaa
    10081 agctgtcgag cggatccagg aactgatggc acaagggatg agcagcggcc aggcaatcgc
    10141 gctggtggca gaagagctgc gtgccaacca ctccggcgag cgaatcgtgg cgcgttttga
    10201 ggatgaagac gagtaacaca ctaatccgta tcgatttcgt tatagaagcc gatgatcatt
    10261 tgattgtgag aaaaaggacg ctttttatat gtgattaaaa gcgtcctgac ccatttatgc
    10321 tgatataaat ttaactcaga ccagtgcttt tagttcttcc tgcatatatg ttgaaaagta
    10381 ttcaggattt ttaatcacag agcgttcacc tgtctccatt ttttttgccc atccaaccat
    10441 tttttccgtg aacccagggt tccagccttc ctggtcagct cgcgccgtaa tatcagcggc
    10501 actcgcgggt actcccagtt cttttaaata tttaaagatt cccttcgccg tgctttcatt
    10561 catcgaactt ggtgttggcg ctgatacatc catggcacca atgaaatcca gggctttatc
    10621 tattacagca ggcatattct tatccttaaa attaaccatg ttatcgataa ggctatatat
    10681 gaccttaatt tgcatctaat atcaaaggga ttataacgat atggcacgag ttaacctgtt
    10741 gacattcagg tgttttaaag gtagcagctc atggtgtgtt atccagtaag atggagaaaa
    10801 ttaacggtca gcccgcacgc tataaccaga ttgttcagct aagaaagaaa accagagttg
    10861 ctaatcaaag ggcgaacaca acggcatggt tatgtttatt tatatagtta gttttataga
    10921 taaaattgaa attataaata taaataatga attagcagaa gcatcaacag taatatctta
    10981 ttctctggtc gatttatgta aaaccgacca gtattatcga taaattatcg tctgacttat
    11041 tttgcttcga taaagcttac accgcagcca caatcttaat ttcaacttta tacttcgggt
    11101 tcatcaaacc cgcttgtacc gtgcagcgca ccggcgcatg acccgcgaca acccaggcat
    11161 cccacgcttt attcatcgcc gcgaagtcgt ttttatcggc caggaaaatg gtggcatcca
    11221 gaatgctcga tttattgctg ccctgttttt ccagcacggc gtcaatctgt gccagcgtgt
    11281 tggcggtttg ctcaaaggca tcggcgtcga ggttttccgg tacaccagtg tagtagagcg
    11341 tgttgttgtg gattactaca tcagaccagc gggcttcagc atcgatacga acgatagtca
    11401 tcattttgtc ctcattatac ttccattttc gtgctgcaaa gactgccaca atgtgaggtt
    11461 gccgtcacca cttcaactgg cgaaagcgcc ctcatcctga cataatccct gttcaaatca
    11521 acagggggta gtgtgacgga cgattttgca ccagacggtc agctggcgaa agcgatacca
    11581 ggctttaagc cgcgagaacc acagcgacag atggcggtag ccgtcaccca ggcgatagaa
    11641 aaaggccagc cgctggtggt ggaagcagga accggtacgg gcaaaaccta cgcttacctg
    11701 gctcctgcgc tgcgggcgaa aaagaaagtc attatctcga ccggctcaaa agcgttgcag
    11761 gatcagctct acagccgcga tttgccaaca gtctcaaagg cattgaaata tacgggcaac
    11821 gtggcgctgc tgaaagggcg ctcaaactac ctctgcctcg aacgtctcga acagcgggcg
    11881 ctggcggggg gcgatctgcc ggtacaaatc ttaagcgatg tgatcctgct gcgctcctgg
    11941 tctaatcaaa cagtcgatgg tgatatcagc acctgcgtca gcgtggcgga agattcacag
    12001 gcgtggccgc tggtcaccag caccaacgac aactgtcttg gcagcgactg cccgatgtat
    12061 aaagattgct ttgtggtcaa agcacgtaaa aaagcgatgg acgccgatgt ggtggtggta
    12121 aaccatcatc tctttctggc ggatatggtg gttaaagaga gtggatttgg cgagttgatc
    12181 cctgaagcgg acgtcatgat cttcgacgaa gcccaccagc taccggacat tgccagccag
    12241 tattttggtc agtcactctc cagtcgacaa ctgctcgacc tggcaaaaga catcaccatc
    12301 gcctaccgca ccgaattaaa agacacccag cagttacaaa agtgcgctga tcgtcttgcc
    12361 cagagtgcgc aggattttcg tctgcaactc ggtgagccag gttatcgcgg taacctgcgt
    12421 gagctgttag ctaatccgca aattcagcgg gcatttttac tgctcgatga caccttggaa
    12481 ctttgttatg acgtggcgaa actgtcactg gggcgttccg ccttgctgga tgcggcattt
    12541 gagcgcgcca cgttgtatcg cacacggctg aagcggctaa aagagatcaa tcagccgggc
    12601 tacagctact ggtacgaatg cacttcgcgc cattttactc tggctctcac gccgctcagc
    12661 gtggcggata aattcaaaga gttaatggcg caaaagcccg gtagctggat cttcacctca
    12721 gcaacgctgt cggtgaacga cgatctgcat catttcacct cgcggcttgg gatagaacag
    12781 gcggagtcgt tgctattgcc aagcccgttt gattacagcc gccaggcgtt actctgtgtg
    12841 ccgcgcaatc tgccgcaaac caaccagcca ggttctgctc gccagttagc ggcaatgctg
    12901 cgaccgatca tcgaagctaa caacggtcgt tgttttatgc tttgtacctc gcacgccatg
    12961 atgcgcgatc tggccgagca gttccgcgct accatgacgc ttcccgtttt gttgcagggg
    13021 gaaaccagta aaggccaact gttgcagcag tttgtcagtg ccggtaatgc gcttcttgtg
    13081 gcaaccagta gtttctggga aggggtggac gtgcgtggcg atacattgtc attggtaatt
    13141 atcgacaaat tgccgtttac ctcgccggat gatccactgt taaaagcgcg catggaagat
    13201 tgtcgtttgc gcggtggcga cccgttcgat gaagtgcaac taccagatgc cgtcattact
    13261 ctcaaacagg gggtaggtcg actgattcgc gacgccgacg atcgtggcgt gctggtgatt
    13321 tgtgactatc ggctggtgat gcgtccttac ggcgcgacgt ttctcgccag tctgccgccc
    13381 gcgccacgca cccgtgacat tgcccgtgcg gttcgtttcc ttgcgatacc atcctccagg
    13441 taatttgtta cggagtatgg tatgatgcgc gcctttttat tgatctaagc acgaagaccc
    13501 atgcgaattc tggctatcga taccgcgaca gaggcctgct ctgtcgccct gtggaacgac
    13561 ggtactgtca acgctcattt tgagctttgc cctcgtgaac atactcaacg aatcttaccg
    13621 atggtgcagg atatcctgac caccagcgga acttccctga ctgatattaa cgctctggct
    13681 tacgggcgcg gccccggtag ctttaccggc gtgcgcattg gtattggcat cgcgcaaggg
    13741 ctggcgcttg gcgcggaatt accgatgatt ggcgtctcca cgctaatgac gatggcgcaa
    13801 ggggcgtggc gcaaaaacgg cgcaacacgc gtgctgtcag ccattgacgc gcgaatgggc
    13861 gaagtttact gggccgaata tcagcgtgat gaaaacggta tctggcacgg tgaagaaacc
    13921 gaagccgtac tcaaacccga actcgttcat gaacgaatgc aacagctttc cggcgaatgg
    13981 gtgacggtag ggacgggctg gcaagcctgg ccggatctcg gtaaagagag cgggctggtt
    14041 ttgcgcgatg gcgaagtgtt actccccgct gcagaagata tgctgccgat tgcttgtcag
    14101 atgtttgccg agggtaaaac ggtggcggtg gaacatgccg agccggttta tttgcgtaac
    14161 aacgtcgcat ggaagaaact tccgggcaaa gaatgaatct taagagtatg ccctgagaaa
    14221 aaaaggagtc gcatcatggc ggttcaaaag aatgttatca aaggcatact ggcaggtacg
    14281 tttgcgctaa tgctgagcgg ttgtgtcact gtgccggacg ccattaaagg cagcagtccc
    14341 acgccgcaac aagatttagt tcgggtgatg agtgcgccgc agctgtacgt tggtcaggag
    14401 gcacgctttg gtggcaaagt ggttgcggta caaaaccagc aagggaaaac ccgcctggaa
    14461 attgctaccg taccgctgga cagcggagcc aggccgacgc tgggagaacc ttctcgcggt
    14521 cgcatttatg ccgatgtgaa cggttttctg gacccggtgg atttccgtgg acaactggtt
    14581 acggtagtcg ggccaatcac cggtgcggtt gacggcaaaa tcggcaatac gccctataaa
    14641 tttatggtga tgcaagtaac gggttacaaa cgttggcatt taaccctgca ggtgattatg
    14701 ccgcctcagc cgattgatcc atggttttat ggcggtcgtg gctggcccta tggctacggc
    14761 ggatggggct ggtataatcc cggccccgcg agagtacaaa cagttgtaac cgaataattg
    14821 cttgttttta aagaaaaaga aacagcggct ggtccgctgt ttctgcattc ttacggtaaa
    14881 gataaaaata aatagtgacg cgcttcgcaa ccttttcgtt gggtaattat caagctggta
    14941 tgatgagtta atattatgtt aacagcatat atatcatttg gggttgcgat gacgacgaac
    15001 acgcatttta gaggtgaaga attgaagaag gtttggctta accgttatcc cgcggacgtt
    15061 ccgacggaga tcaaccctga ccgttatcaa tctctggtag atatgtttga gcagtcggtc
    15121 gcgcgctacg ccgatcagcc tgcgtttgtg aatatggggg aggtgatgac cttccgcaag
    15181 ctggaagaac gcagtcgcgc gtttgccgct tatttgcaac aagggttggg gctgaagaaa
    15241 ggcgatcgcg ttgcgttgat gatgcctaac ttattgcaat atccggtggc gctatttggc
    15301 attttgcgta ccgggatgat cgtcgtaaac gttaacccgt tgtatacccc gcgtgagctt
    15361 gagcatcagc ttaacgatag cggcgcatcg gcgattgtta tcgtgtctaa ctttgcccac
    15421 actctggaaa aagtggttga taaaaccgcc gttcagcacg taattctgac ccgtatgggc
    15481 gatcagctat ctacggcaaa aggcacggta gtcaatttcg ttgttaaata catcaagcgt
    15541 ttggtgccga aataccatct gccagatgcc atttcatttc gtagcgcact gcataacggc
    15601 taccggatgc agtacgtcaa acccgaactg gtgccggaag atttagcctt cctgcaatac
    15661 accggcggca ccacaggtgt ggcgaaaggc gcgatgctga ctcaccgcaa tatgctggcg
    15721 aacctggaac aggtcaacgc cacctatggg ccgttgctgc atccaggtaa agaactggta
    15781 gtaacggcac tgccgctgta tcacattttt gccctgacca ttaactgcct gctgtttatc
    15841 gaactgggtg ggcagaacct gcttatcact aacccgcgtg atattccagg gttggtaaaa
    15901 gagttagcga aatatccgtt taccgctatc actggcgtta acaccttgtt caatgcgttg
    15961 ctgaacaata aagagttcca gcagctggat ttctccagtc tgcatctttc cgcaggcggt
    16021 gggatgcctg ttcagcaagt ggtggcagaa cgctgggtga aactgaccgg acagtatctg
    16081 ctggaaggtt atggcctgac cgagtgcgcg ccgctggtga gcgttaaccc gtatgatatt
    16141 gattatcata gcggtagcat cggtttgccg gtgccatcga cggaagccaa actggtggat
    16201 gacgacgaca acgaagtacc accgggtcaa ccgggtgagc tttgtgtcaa aggaccgcag
    16261 gtgatgctgg gttactggca gcgtccggat gctacagatg agatcatcaa aaatggctgg
    16321 ttacacaccg gcgacatcgc ggtgatggat gaagaaggat tcctgcgcat tgtcgatcgt
    16381 aaaaaagaca tgattctggt ttccggtttt aacgtctatc ccaacgagat tgaagatgtc
    16441 gtcatgcagc atcctggcgt acaggaagtc gcggctgttg gcgtaccttc cggctccagt
    16501 ggtgaagcgg tgaaaatctt cgtagtgaaa aaagatccat cgcttaccga agagtcactg
    16561 gtgacttttt gccgccgtca gctcacggga tacaaagtac cgaagctggt ggagtttcgt
    16621 gatgagttac cgaaatctaa cgtcggaaaa attttgcgac gagaattacg tgacgaagcg
    16681 cgcggcaaag tggacaataa agcctgagcg ttaagtcagt cgtcagacgc cggttaatcc
    16741 ggcgtttttt ttgacgccca ctaaagagaa aacaatttga attaccaaat gattaccacg
    16801 gacgatgcgc tggcttcttt gtgtgaagcc gtccgtgcct ttccggcgat agccctggat
    16861 actgaatttg ttcgtacgcg cacttattac ccgcagctgg ggttgattca acttttcgat
    16921 ggcgagcatc tggcgctaat tgatcctctc gggatcaccg actggtcacc gctgaaagcg
    16981 atcctgcgcg atccgtccat cacaaaattt ctccatgcag gcagtgaaga tctggaagtg
    17041 ttcctcaatg tctttggcga attaccacaa cccttgattg acacgcaaat ccttgctgcc
    17101 ttctgcggac gcccgatgtc atggggtttc gcttccatgg tggaagagta ttccggcgtt
    17161 acgctggaca agagtgaatc gcgcaccgac tggctggcca gaccgctgac cgaacgtcag
    17221 tgtgaatacg cagcggcgga tgtctggtat ctgttaccga tcaccgccaa gcttatggta
    17281 gaaacggagg cctccggctg gctacctgcg gcgctggatg aatgccgcct gatgcaaatg
    17341 cgtcgtcagg aagtcgttgc gccggaagat gcctggcgtg atatcaccaa tgcctggcag
    17401 ttacgcacac gccaactggc ctgtctgcaa ctgttagccg actggcgact gcgcaaggcg
    17461 cgagagcgtg atctggcggt gaactttgtc gtgcgtgaag agcatttgtg gtcggtcgcg
    17521 cgttatatgc cgggaagttt aggcgaactg gacagcctgg gtttatccgg tagcgaaatc
    17581 cgctttcacg gtaaaacgct gctggcgctg gtggaaaaag cgcagacatt gccggaagat
    17641 gccttaccgc agccgatgct taacctgatg gacatgcctg gttatcgtaa agcgtttaaa
    17701 gcgattaagt cgctgattac tgacgtgagc gaaacgcata agatcagcgc cgaattgctg
    17761 gcatcgcgtc ggcaaatcaa ccaactgctg aactggcact ggaaactgaa accgcagaac
    17821 aatttgccgg agctgatttc cggctggcgt ggtgagctga tggcggaagc attacacaat
    17881 ttattgcagg aatatccgca gtaaaatctt ccgaagccgg actgggcgcg ctcagcgcca
    17941 catccggctt tggcaaacta caaatccaac accaggcgtt tacccttcgc acgcgaacaa
    18001 cagatcaaca tactttgctg gctggcacgc tcttcatcgc taaaatactg atcccgatgg
    18061 tcagcttcac cttccagtat tgccgtttcg caggttccgc ataccccttc acgacataaa
    18121 cattccactt tcgcggcttt attattttca ataacctgca aaatagtcat ctcttccggc
    18181 accacaaact cttttccgga acgggcaagc accagggtaa atgcatcgcc ggttttgtct
    18241 tcgatagcaa attgctcaaa gtgcagcgta tcggcggcga tgtccagacg cgcagcttca
    18301 cttcttaccg cttcaattag cgcctcgggg ccacaggtat aaacgtgtgt gccaggttcg
    18361 atatccgcca ataatcgagc taattccagt cgtgttccgg ttgatgaatg atgcaaatgg
    18421 actttctcag cctgcggatg ctggactagc tcatcacgaa atgcgcaact ttctggattt
    18481 cgcgagcagt aatgtagctg ccagtcgacg tcgctgtgtt gcaactctgc catgtgcgac
    18541 aggaaagggg tgataccaat accgcccgcg ataaacagat gctttctggc tgagggaatt
    18601 agcgcaaaca ggttattagg cgttgagatc gttaaccgat cgcccacttt tacctgctga
    18661 tgcaaaaagc gggaaccgcc gcgcgagttt tcttccagtc gaacggcaat ctgataacta
    18721 gaggtgttat gcggtgaact cagtagtgaa tacgcattgc tgtactggtt atcaccatcg
    18781 ctcatctgca ctaggatgtg acttcctccg gtgaacgcag gcagcggttt gccatcggtt
    18841 gccaccagcg tgaagcgttt cacctgttcg gtaaggggtt caacctggct cacctgtact
    18901 tcaaacattt gatagtctga cattgctgta ctccatcaaa aatcagccca gcggcggaaa
    18961 ataccgccgc cgatgtcatt agtccttaaa cacctgcgcc agcagattat ggaaatgggc
    19021 gataccatgt tcggaaatgc cactaccgct actgtcggcc atgatgcgcc cctgaccacg
    19081 atagccacgc gatttcagcc ctttctgtac gctttcaacc agacgtaaat cttccggatg
    19141 gaacacatcg cgataccact caatcagcga tttttgctcg tcggttaact cttcattggt
    19201 gaagtaaata tcgtagtttt gcagggtagt ttcagaatcc accgggaatt cataaatgac
    19261 cgtcatcatc cctttgatcg gggtgacgtt cagcatcgtg cacggccaca gccagaaacc
    19321 gtggaatgcc gcatccgtac cctcttcaaa tttaaacgac tgttcggacg gtttggcgaa
    19381 accgtattgc agcgtccagt taccgtgcat ggtgtgccag taacgatcaa cctgtacgga
    19441 gtcggagaaa cctggatgcg ccggaccaca gtgatagcac tcgagatagt tatcgacaat
    19501 gttcttccag ttggcaggcg tgcgggtggt aaagcgggcc gctagtttca gattgtggac
    19561 ttccgggcag gcttccagca ctttcgcgcc cagaccggtg tattgattca cttgaagtac
    19621 gaaaaaaaac cgggaggaca ttggattatt cgggttctga tgggattaga tttggtgggg
    19681 cttgcaagcc tgtagcgcaa attttagtcg ttaatcaatg aaacgcgaaa gatagtaaaa
    19741 aattgctttt gtttcattga aaatacgaaa aacaaaaaca ctgcaaatca tttcaataac
    19801 agcttcaaaa aacgttcaaa accgataaca accatgctgt aaccaaatga ctcatatcac
    19861 aaatcagctc atgccgttta ggtatgttac atgtgtgatt atgtgaggtg aagtatgttt
    19921 tagctggttc atggttgtta tacggctttt tttacatctt gtggttcttg tgaaggagct
    19981 acaagacctt cctgttcata aataccatac tttgacaaaa tctctttacg agtatctgtt
    20041 ggtaatgatt caagcatttt ggctgtttgt aaggtttgct gcgaaatacc ttgcaaatca
    20101 gcctggatgg aatcatcgga agaaaaaatc tcaggcataa gagcttcaaa tcctttcttt
    20161 ctgaacaact caatcgcaga atgcagttcc ttgtcctcta acaattcgac aattgcgatc
    20221 agtttagttt tcaatgtgct gcggtcaggt tgaggatttt tattctcttg agtatttttg
    20281 tgctcaacca ttggcatgct ctgactttgg ataaaccact ctattggacg ccctgttgcg
    20341 taggctattt gtgctaatcg atctaaagac gggtatgtct tgccagacag gtagtttctg
    20401 ataaccccat cagaaagtcc gcacttagct gcaaacgccc
//
LOCUS       DBKEKI010000063        20089 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.063, whole genome shotgun sequence.
ACCESSION   DBKEKI010000063 DBKEKI010000000
VERSION     DBKEKI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.063"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..122
                     /locus_tag="ACUQPX_002327"
                     /pseudo
     CDS             1..122
                     /locus_tag="ACUQPX_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            523..1044
                     /gene="aroK"
                     /locus_tag="ACUQPX_002328"
     CDS             523..1044
                     /gene="aroK"
                     /locus_tag="ACUQPX_002328"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HHV9057025.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            1101..2189
                     /gene="aroB"
                     /locus_tag="ACUQPX_002329"
     CDS             1101..2189
                     /gene="aroB"
                     /locus_tag="ACUQPX_002329"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HHV9057026.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            2281..3567
                     /gene="damX"
                     /locus_tag="ACUQPX_002330"
     CDS             2281..3567
                     /gene="damX"
                     /locus_tag="ACUQPX_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HHV9057027.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTELAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQVDLK"
     gene            3674..4510
                     /gene="dam"
                     /locus_tag="ACUQPX_002331"
     CDS             3674..4510
                     /gene="dam"
                     /locus_tag="ACUQPX_002331"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HHV9057028.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            4528..5205
                     /gene="rpe"
                     /locus_tag="ACUQPX_002332"
     CDS             4528..5205
                     /gene="rpe"
                     /locus_tag="ACUQPX_002332"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HHV9057029.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            5198..5956
                     /gene="gph"
                     /locus_tag="ACUQPX_002333"
     CDS             5198..5956
                     /gene="gph"
                     /locus_tag="ACUQPX_002333"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HHV9057030.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            5949..6953
                     /gene="trpS"
                     /locus_tag="ACUQPX_002334"
     CDS             5949..6953
                     /gene="trpS"
                     /locus_tag="ACUQPX_002334"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HHV9057031.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRMLKAVYEAIGFVAKP
                     "
     gene            7223..8128
                     /gene="yhfZ"
                     /locus_tag="ACUQPX_002335"
     CDS             7223..8128
                     /gene="yhfZ"
                     /locus_tag="ACUQPX_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HHV9057032.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            8138..8437
                     /locus_tag="ACUQPX_002336"
     CDS             8138..8437
                     /locus_tag="ACUQPX_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001430106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HHV9057033.1"
                     /translation="MMQVVNVLEKECHLPVRSEQGTMAMTHMASALMRSRRGEEIEPL
                     DNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEEGYLLANLYGLWMAANEEV"
     gene            8521..9684
                     /locus_tag="ACUQPX_002337"
     CDS             8521..9684
                     /locus_tag="ACUQPX_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HHV9057034.1"
                     /translation="MFVEELKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRGQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            9685..10911
                     /locus_tag="ACUQPX_002338"
     CDS             9685..10911
                     /locus_tag="ACUQPX_002338"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HHV9057035.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQTLVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAECLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            10908..11786
                     /locus_tag="ACUQPX_002339"
     CDS             10908..11786
                     /locus_tag="ACUQPX_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HHV9057036.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            11797..12150
                     /locus_tag="ACUQPX_002340"
     CDS             11797..12150
                     /locus_tag="ACUQPX_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HHV9057037.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            12162..13466
                     /locus_tag="ACUQPX_002341"
     CDS             12162..13466
                     /locus_tag="ACUQPX_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HHV9057038.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDANGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            13478..14551
                     /locus_tag="ACUQPX_002342"
     CDS             13478..14551
                     /locus_tag="ACUQPX_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HHV9057039.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLATLRATG
                     VPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRATLYS
                     GGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANAQSKV
                     LIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLEHCAI
                     RINPNRSGEETVLRILRESIASI"
     gene            complement(14711..15442)
                     /gene="frlR"
                     /locus_tag="ACUQPX_002343"
                     /gene_synonym="yhfR"
     CDS             complement(14711..15442)
                     /gene="frlR"
                     /locus_tag="ACUQPX_002343"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HHV9057040.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQTGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(15542..16327)
                     /gene="frlD"
                     /locus_tag="ACUQPX_002344"
     CDS             complement(15542..16327)
                     /gene="frlD"
                     /locus_tag="ACUQPX_002344"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HHV9057041.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(16324..17154)
                     /gene="frlC"
                     /locus_tag="ACUQPX_002345"
     CDS             complement(16324..17154)
                     /gene="frlC"
                     /locus_tag="ACUQPX_002345"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HHV9057042.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(17204..18226)
                     /gene="frlB"
                     /locus_tag="ACUQPX_002346"
     CDS             complement(17204..18226)
                     /gene="frlB"
                     /locus_tag="ACUQPX_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HHV9057043.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(18247..19479)
                     /locus_tag="ACUQPX_002347"
                     /pseudo
     CDS             complement(18247..19479)
                     /locus_tag="ACUQPX_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(19770..19937)
                     /locus_tag="ACUQPX_002348"
     CDS             complement(19770..19937)
                     /locus_tag="ACUQPX_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HHV9057044.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         4761 a         4959 c         5573 g         4796 t
ORIGIN      
        1 atagcgtacc gttgcttggc gacattccct agttcgggca attatttcgc catgacggaa
       61 aagaagatga acgacgcgag ttagtggtgt ttatcacgcc acgactggtt tccagtgagt
      121 aaacagccgt aaaagcggta atgtttttat gctgaacgtg tttcatctat ttgacgcgcg
      181 caggtattta gcatacaagg agtaccgatt tgagagttgg tgctcttcgc tgcctgcgtt
      241 ccatgatgat gatttatcat tcaggcggca ttttgctgtc ttttttacgc taatcttacc
      301 cggtgattta tcgccagagc ggtggtagca aggcagcgcg cttgcagcga ccagatatgc
      361 agagggatgg gtgatttatt cagttgccaa acccgctgga gtattgagat aattttcagt
      421 ctgactctcg caatatctta tgaggtttca gttcatgtcc tgcggcgctc tctgagcgaa
      481 gcgggtttat cattaacgaa tagtcttagt agtaccgaaa aaatggcaga gaaacgcaat
      541 atctttctgg ttgggcctat gggtgccgga aaaagcacta ttgggcgcca gttagctcaa
      601 caactcaata tggaatttta cgattccgat caagagattg agaaacgaac cggagctgat
      661 gtgggctggg ttttcgattt agaaggcgaa gaaggcttcc gcgatcgcga agaaaaggtc
      721 atcaatgagt tgaccgagaa acagggtatt gtgctggcta ctggcggcgg ctctgtgaaa
      781 tcccgtgaaa cgcgtaaccg tctttccgct cgtggcgttg tcgtttatct tgaaacgacc
      841 atcgaaaagc aacttgcacg cacgcagcgt gataaaaaac gcccgttgct gcacgttgaa
      901 acaccgccgc gtgaagttct ggaagcgttg gccaatgaac gcaatccgct gtatgaagag
      961 attgccgacg tgaccattcg tactgatgat caaagcgcta aagtggttgc aaaccagatt
     1021 attcacatgc tggaaagcaa ctaattctgg ctttatatac actcgtctgc gggtacagta
     1081 attaaggtgg atgtcgcgtt atggagagga ttgtcgttac tctcggggaa cgtagttacc
     1141 caattaccat cgcatctggt ttgtttaatg aaccagcttc attcttaccg ctgaaatcgg
     1201 gcgagcaggt catgttggtc accaacgaaa ccctggctcc tctgtatctc gataaggtcc
     1261 gcggcgtact tgaacaggcg ggtgttaacg tcgatagcgt tatcctccct gacggcgagc
     1321 agtataaaag cctggctgta ctcgataccg tctttacggc gttgttacaa aagccgcatg
     1381 gtcgcgatac tacgctggtg gcgcttggcg gcggcgtagt gggcgatctg accggcttcg
     1441 cggcggcgag ttatcagcgc ggtgttcgtt tcattcaagt cccgacgacg ttactgtcgc
     1501 aggtcgattc ctccgttggc ggcaaaactg cggtcaacca tcccctcggt aaaaacatga
     1561 ttggcgcgtt ctaccagcct gcttcagtgg tggtggatct cgactgtctg aaaacgcttc
     1621 ccccgcgtga gttagcgtcg gggctggcag aagtcatcaa atacggcatt attcttgacg
     1681 gtgcgttttt taactggctg gaagagaatc tggatgcgtt gttgcgtctg gacggtccgg
     1741 caatggcgta ctgtattcgc cgttgttgtg aactgaaggc agaagttgtc gccgccgacg
     1801 agcgcgaaac cgggttacgt gctttactga atctgggaca cacctttggt catgccattg
     1861 aagctgaaat ggggtatggc aattggttac atggtgaagc ggtcgctgcg ggtatggtga
     1921 tggcggcgcg gacgtcggaa cgtctcgggc agtttagttc tgccgaaacg cagcgtatta
     1981 taaccctgct cacgcgggct gggttaccgg tcaatgggcc gcgcgaaatg tccgcgcagg
     2041 cgtatttacc gcatatgctg cgtgacaaga aagtccttgc gggagagatg cgcttaattc
     2101 ttccgttggc aattggtaag agtgaagttc gcagcggcgt ttcgcacgag cttgttctta
     2161 acgccattgc cgattgtcaa tcagcgtaac aacaagaaag gtcaggccgc ttatcaagcg
     2221 gtctattagc ttcaggttaa ttgcaacgtg gtaagcatta accttttagt ggggtgttaa
     2281 atggatgaat tcaaaccaga agacgagctg aaacccgatc ccagcgatcg tcgtactggt
     2341 cgttctcgtc aatcttctga acgttctgag cgtactgaac gtggcgaacc gcagatcaat
     2401 tttgatgata ttgaacttga tgacactgac gatcgccgtc cgactcgtgc gcaaaaagag
     2461 cgcaatgagg aaccggaaat cgaagaagaa attgacgaat ccgaagatga aaccgtggat
     2521 gaagagcgcg tagagcgtcg tccgcgtaag cgcaaaaaag cagccagtaa acccgcttct
     2581 cgtcagtata tgatgatggg cgtcggcatt ctggttctac tgctgttgat catcggtatc
     2641 ggttctgcgc taaaagcccc ctcgaccact tccagcgatc aaaccgcgtc tggcgagaag
     2701 agtattgatc ttgctggcaa tgcgaccgat caggcgaatg gcgtgcagcc agcgccggga
     2761 accacgtctg cggaaaatac tcagcaggat gtttctctgc caccgatctc ttctacgccg
     2821 actcaagggc aaaccccggt ggcaacggat ggtcaacaac gtgttgaagt gcagggtgac
     2881 ctgaacaatg cgctgaccca gccacaaaat cagcaacagc tgaacaatgt ggcggtcaat
     2941 tccacgttgc cgactgaacc cgcaacggtt gcgcctgttc gcaatggcaa tgcatcgcgt
     3001 gacacggcga aaacgcaaac cgctgaacgt ccgtccacta cgcgcccagt tcgtcagcag
     3061 gcggtgattg aaccgaaaaa accgcaagca accgtgaaaa cggagccgaa gccggtagca
     3121 cagacaccga agcgtactga actagctgcc cctgtggcga gcacgaaggc accggctgcg
     3181 acttctacgc cagcaccaaa agagacggcg actacggctc cagtacagac ggcatccccg
     3241 gcgcaaacca cggcaacacc agccgctgga gggaagaccg caggtaatgt tggttcgttg
     3301 aaatcggcac cgtccagcca ttacactctg cagctgagca gttcctctaa ctacgacaac
     3361 ctgaacggtt gggcgaagaa agagaatctg aaaaactacg ttgtctatga aacgacgcgt
     3421 aatggtcagc cgtggtatgt cctggtttct ggcgtgtacg cttcgaaaga agaggcgaaa
     3481 aaagcggtat ctacattgcc agcagatgtt caggccaaaa acccgtgggc gaaaccgctg
     3541 cgtcaggtac aggtcgatct gaagtaatca aggttatctc ccgcaatggt ttatcgttgc
     3601 gggagctgcc tgaagcgctg gatgctgtcg gagctttctc cacagccgga gaaggtgtaa
     3661 ttagttagtc agcatgaaga aaaatcgcgc ttttttgaag tgggcagggg gcaagtatcc
     3721 cctgcttgat gatattaaac ggcatttgcc caagggcgaa tgtctggttg agccttttgt
     3781 aggtgccggg tcggtgtttc tcaacaccga cttttctcgt tatatccttg ccgatatcaa
     3841 tagcgacctg atcagtctct ataacattgt gaagatgcgt actgatgagt acgtacaggc
     3901 cgcacgcgag ctgtttgttc ccgaaacaaa ttgcgccgag gtttactatc agttccgcga
     3961 agagttcaac aaaagccagg atccgttccg tcgggcggta ctgtttttat atttgaaccg
     4021 ctacggttac aacggcctgt gtcgttacaa tctgcgcggt gagtttaacg tgccgtttgg
     4081 ccgctacaaa aaaccctatt tcccggaagc agagttgtat cacttcgctg aaaaagcgca
     4141 gaatgccttt ttctattgtg agtcttacgc cgatagcatg gcgcgcgcag atgatgcatc
     4201 cgtcgtctat tgcgatccgc cttatgcacc gctgtctgcg accgccaact ttacggcgta
     4261 tcacacaaac agttttacgc ttgaacaaca agcgcatctg gcggagatcg ccgaaggtct
     4321 ggttgagcgc catattccag tgctgatctc caatcacgat acgatgttaa cgcgtgagtg
     4381 gtatcagcgc gcaaaattgc atgtcgtcaa agttcgacgc agtataagca gcaacggcgg
     4441 cacacgtaaa aaggtggacg aactgctggc tttgtacaaa ccaggagtcg tttcacccgc
     4501 gaaaaaataa ttctcaagga gaagcggatg aaacagtatt tgattgcccc ctcaattctg
     4561 tcggctgatt ttgcccgcct gggtgaagat accgcaaaag ccctggcagc tggcgctgat
     4621 gtcgtgcatt ttgacgtcat ggataaccac tatgttccca atctgacgat tgggccaatg
     4681 gtgctgaaat ccttgcgtaa ctacggcatt accgccccta tcgacgtaca tctgatggtg
     4741 aaacccgtcg atcgcattgt gcctgatttc gctgccgctg gtgccagcat cattaccttt
     4801 catccagaag cctccgagca tgttgaccgc acgctgcaac tgattaaaga aaatggctgt
     4861 aaagcgggtc tggtatttaa cccggcgaca cctctgagct atctggatta cgtgatggat
     4921 aagctggatg tgatcctgct gatgtccgtc aaccctggtt tcggcggtca gtctttcatt
     4981 cctcaaacac tggataaact gcgcgaagta cgtcgccgta tcgacgagtc tggctttgac
     5041 attcgtctgg aagtggacgg tggcgtgaag gtgaacaaca ttggcgaaat cgctgcggcg
     5101 ggcgcggata tgttcgtcgc cggttcggca atcttcgacc agccagacta taaaaaagtc
     5161 attgatgaaa tgcgcagtga actggcaaag gtaagtcatg aataagtttg aagatattcg
     5221 cggcgtcgct tttgatcttg atggtacgct ggtcgacagt gctcctggtc ttgctgctgc
     5281 ggtagatatg gcgctgtatg cgctggagtt gcccgtcgca ggtgaagaac gcgttattac
     5341 ctggattggt aacggcgcag atgttctgat ggagcgcgca ttgacctggg cgcgtcagga
     5401 acgtgcgact ctgcgtaaaa caatggggaa accgcccgtt gatgacgaca ttccggcaga
     5461 agaacaggta cgtattctgc gtaaactgtt cgatcgctac tatggcgagg ttgccgaaga
     5521 ggggacgttt ttgttcccgc acgttgccga tacgttgggc gcgttgcagg ctaaaggcct
     5581 gccgctaggc ctggtcacca acaaaccgac gccgttcgtc gcgccgctgc tcgaagcctt
     5641 agatattgcc aaatacttta gcgtggttat cggcggcgat gatgtgcaaa acaaaaaacc
     5701 gcatccggac ccgctgttac tggtggctga gcggatggga attgccccac aacagatgct
     5761 gtttgtcggc gactcacgca atgatattca ggcggcaaaa gcggcaggtt gcccatcagt
     5821 tggcttaacc tacggatata actacggcga ggctatcgat ctcagccagc ctgatgtaat
     5881 ttatcagtct ataaatgacc ttctgcccgc attagggctt ccgcatagcg aaaatcagga
     5941 atcgaaaaat gactaagccc atcgttttta gtggcgcaca gccctcaggt gaattgacca
     6001 ttggtaacta catgggtgcg ctgcgtcagt gggtaaacat gcaggatgac taccattgca
     6061 tttactgtat cgttgaccaa cacgcgatca ccgtgcgcca ggatgcacag aagctgcgta
     6121 aagcgacgtt ggatacgctg gccttgtatc tggcttgtgg tatcgatcct gagaaaagca
     6181 ccatttttgt tcagtcccac gtaccagaac atgcgcagtt aggctgggca ctgaactgct
     6241 atacctactt cggcgaactg agtcgcatga cgcagtttaa agataaatct gcgcgttatg
     6301 ccgagaacat caacgctggt ctgtttgact atccggtgct gatggctgcg gacatcctgc
     6361 tgtatcaaac taatctggta ccggtgggtg aagaccagaa acagcacctg gaactgagtc
     6421 gcgatatcgc ccagcgtttc aacgcgctgt atggcgagat ctttaaggtg ccggagccgt
     6481 ttattccgaa atctggcgcg cgcgtaatgt cgctgctgga gccgaccaag aagatgtcca
     6541 agtctgacga taaccgcaat aacgttatcg gcctgctgga agatccgaaa tcggtagtga
     6601 agaaaatcaa acgcgcggtc actgactccg acgagccgcc ggtagttcgc tacgatgtgc
     6661 agaacaaagc gggcgtttcc aacctgctgg atatcctttc tgcggtaacg ggccagagca
     6721 tcccagaact ggaaaaacag ttcgaaggca agatgtatgg tcatctgaaa ggtgaagtgg
     6781 ctgatgccgt ttccggtatg ctgactgaat tgcaggaacg ctatcaccgt ttccgcaacg
     6841 atgaagcctt cctgcaacag gtgatgaaag acggcgcaga aaaagccagt gtacacgctt
     6901 cccgtatgct gaaagcggtg tacgaagcga ttggttttgt ggcgaagccg taatatcaaa
     6961 acgctcggac ggtcccctcg cccctttggg gagagggtta gggtgagggg agcaggcaag
     7021 cgccagtggg aattaccctt tgctcgcatt gtcgaataaa tcacttcgtc atcactctga
     7081 aaccggggaa acccggtttt tttatcctct tttcgcaaca acccgaaaaa atgtgaagcg
     7141 cctcgccgtt tccacatctc gtcgttgcca cttatcttca ttttaatgaa gatataaata
     7201 tttattttat tgaaaatata ttatgcgtcg aacgtttatc aaaaaagaag gcgtcgtcat
     7261 cacgacactg gcccgttatt tgttgggtga aaaatgcggt aatcgactga aaaccataga
     7321 tgagctggca actgaatgcc gttcatcggt tggcctgacg caggccgcgt tgaaaacgct
     7381 ggaatcaagc ggagcgatac ggattgaacg ccgtgggcgc aatggcagtt atcttgtcga
     7441 gatggataac aaagcattgc tgagtcatgt ggatatcaac aacgtggtat gtgcgatgcc
     7501 tttgccctat acccgcttgt acgaaggttt agcgagcgga ttgaaagccc agtttgatgg
     7561 cattcctttt tactatgcgc atatgcgcgg cgcggatatt cgcgtggagt gtttacttaa
     7621 cggtgtctat gacatggcag tagtttcccg cctggcggcg gaaagttatc tcagccaaaa
     7681 caatttgtgc atcgccctcg agcttggccc gcatacgtat gttggtgaac atcaactgat
     7741 atgccgcaaa ggtgagtctg gaaatgttaa gcgagtaggt ctcgatagtc gttcggcgga
     7801 tcagaaaatc atgaccgatg tcttttttgg cgatagtgat gtggaacgag tcgatctctc
     7861 ttatcacgag agtttacaac gcattgttaa aggcgatgtc gatgcggtga tctggaacgt
     7921 ggtggcggaa aacgaactga ccatgctggg attagaggcg acgccgctaa cagacgatcc
     7981 gcgattttta caggccaccg aagccgtggt cctgacgcga gtcgatgatt acccaatgca
     8041 acaattgctg cgtgccgttg tagataaaca cgccctgctt gcccatcaac aacgggtggt
     8101 gagtggggaa caggaaccca gttattaaac gaaaggcatg atgcaggtcg tcaacgtact
     8161 ggaaaaagag tgccatctgc cggtgcgcag tgagcaagga acgatggcga tgacacatat
     8221 ggcgagtgca ctgatgcgca gtcgccgtgg tgaagaaata gagccgctgg ataacgagtt
     8281 gctggcggaa ctggcgcaat ccagccactg gcaagccgtt gtgcaattgc atcaggtgtt
     8341 gttgaaggaa ttcgctctgg aagttaaccc gtgtgaagaa ggctatttgc tggccaatct
     8401 ttatggatta tggatggctg ctaacgaaga ggtttgaagc tcccgacaaa tccacgtcgc
     8461 acaacctaaa tattcaaaaa aatgaatatt taattcaagg aacagaaacg aggcaacaag
     8521 atgtttgtag aagaattgaa acgccagaac ccggcgctga tttccgccgc actaagtttg
     8581 tggcagcagg gcaaaatcgc cccggacagc tgggtgatcg acgtggatca gatactggaa
     8641 aacggtaagc ggctcattga gacggcgcgg ctttacggca ttgaactgta tctgatgacc
     8701 aagcaatttg gtcgtaatcc gtggctggcg gaaaaattgc tggcattagg ctacagcggc
     8761 attgtggcgg tggattacaa agaggcgcga gtcatgcgcc gcgctggttt gccagtggcg
     8821 catcaggggc atctggtaca aatcccttgt catcaggttg ctgacgccgt tgaacagggc
     8881 accgacatca tcaccgtgtt tactctcgac aaagcgcggg aagtttctgc ggcagcggtt
     8941 aaggccggac ggggccagcc tgtgctgctc aaagtttata gcgacgatga ttttctttat
     9001 ccggggcagg agagcggttt tgtacagcat tcgctgcacg aggtgattgc tgaaattaag
     9061 aaactgccgg ggctgcattt agccggacta acccatttcc cttgcctgct ttgggatgag
     9121 gctgccggga aagttttgcc aacaccgaat cttcacacgc tggtacaagc acgggatcaa
     9181 ctggcgaaat ctggtattgc gattgagcaa ctgaacgtac cttcagcgac cagctgcact
     9241 tcgctgccat tgctggcgga atacggtgcg actcatgccg aacccggtca tgcgctgacc
     9301 ggtactattc cggcaaacca gcagggcgat cagcctgaac gtatcgcgat gctctggtta
     9361 agtgaaatct cccatcattt ccgtggcgac agctactgct acggcggcgg ttactaccgt
     9421 cgtggtcatg cgcaacatgc gctggtgttt acgccagaaa atcaaaagat tactgaaacc
     9481 aatctcaaaa ctgtggatga cagcagtatc gactacaccc tgccgctggc aggcgagtat
     9541 ccggtgagca gtgcagtggt gctctgtttt cgcacgcaga tttttgtcac ccgtagcgat
     9601 gtggtgctgg tgtccggtat tcagcgcggc gaaccagaaa tagttggtcg ttatgacagc
     9661 ctcggcaacc ctctggaggc ataaatggcg cgatttgtgg tgttagtgat tgatagcttt
     9721 ggcgtagggg caatgaaaga tgtcacgctg gtgcgcccgc aagatgccgg ggcgaatacc
     9781 tgtggtcaca tcctgagcca gttgccgcat ttgcagctac caacgctgga gaagctgggg
     9841 ctaatcaaca cattgggtta tgcgccaggc gatatgcagc cgtcagattc cgcaacctgg
     9901 ggcgtggcag agctgcaaca tgaaggtggc gataccttta tggggcatca ggaaatttta
     9961 ggcacgcgcc cgttaccgcc gctgcggatg ccttttcgcg atgtgattga ccgtgttgag
    10021 cagacattag tttccgctgg ctggcaggtg gagcgccgtg gcgatgatct gcaatttctg
    10081 tgggtcaatc aggcggttgc gattggcgat aatctcgagg cggatttagg ccaagtctat
    10141 aacattaccg ccaatctctc tgtgatctct tttgacgacg caatcaaaat tggtcgtatc
    10201 gtgcgtgacc aggtacaggt cggtcgggtc attacatttg gcggcctgtt aaccaacagt
    10261 cagcgtattc tcgatgccgc agaaagcaaa gaagggcgct ttattggtat taatgcgccg
    10321 cgttctggcg cttatgacaa cggcttccag gtcgtgcata tgggctacgg tgttgatgaa
    10381 aaagtgcagg tgccgcaaaa actgtatgaa gcgggcgtgc caaccgtgct ggtgggtaag
    10441 gtggcagata tcgtcagcaa tccttatggt gtgagctggc aaaacctggt ggatagccag
    10501 cggattatgg atatcaccct cgacgaattt aacacccatc cgacggcgtt tatttgcatc
    10561 aatatccagg aaaccgacct cgctggtcat gcagaagacg tcgcacgtta tgccgaatgt
    10621 ttgcaggtcg ttgaccgtaa ccttgcccgg cttgttgagg cgatgcagcc agatgattgc
    10681 ctggtcgtga tggcggatca cggtaacgat ccgaccattg gtcacagcca ccatacccgt
    10741 gaagtggtgc cagtgctggt ttatcagcaa gggctggtcg ccacgcagct cggtgtgcgc
    10801 accacgcttt ctgatgtggg agctaccgtg tgtgaatttt tccgtgcgcc accgccacaa
    10861 aatggtcgct cttttctttc ctccctccgg tttgcaggag acaccctatg agttttgatc
    10921 cgacgggtta caccctggcc catgagcatc tgcatattga tctctccggc tttaaaaaca
    10981 acgtggactg ccgccttgat cagtatgcgt tcatttgcca ggagatgaac gacctgatga
    11041 cccggggtgt acgtaatgtg attgagatga ccaaccgtta catggggcgc aatgcgcaat
    11101 ttatgctcga tgtaatgcgc gaaaccggga tcaacgtggt ggcctgtact ggttattacc
    11161 aggatgcgtt tttcccggaa catgtggtga cccgcagcgt gcaggaactg gcgcaggaga
    11221 tggtcgatga aattgaacag ggtatcgatg gcacggagct gaaagccggg atcatcgcgg
    11281 agatcggctc cagcgaagga aagattacgc cgctggaaga gaaggtattt attgccgctg
    11341 cgctggcgca taaccagacc ggacgcccga tctccacgca tacgtcgttc agcaccatgg
    11401 ggctggaaca actggctctg ttgcaagccc acggagttga tctttcgcgc gtcaccgttg
    11461 gtcactgcga tctgaaagac aacctcgaca acattttgaa gatgatcgat ctcggcgcgt
    11521 acgtgcagtt cgacaccatt ggtaagaaca gttactaccc ggacgaaaag cgcattgcga
    11581 tgctccacgc gctacgtgac cgtgggttgc tgaaccgcgt catgctgtcg atggatatta
    11641 cgcgccgctc ccatttaaaa gccaatggtg gttatggcta tgactattta ttgaccacct
    11701 ttattccgca attgcgccag tcaggattca gtcaggccga tgtggatgtg atgttacgtg
    11761 aaaatccctc tcaatttttc caataaggac agactcatga aaaagattgg cgttgcaggc
    11821 ttacagcgtg agcagattaa aaaaaccatt gaagcgacgg ctcctggctg ttttgaagtt
    11881 ttcattcaca acgacatgga agcggcaatg aaggtgaaat ccgggcaact agattattac
    11941 attggcgcgt gtaataccgg tgcgggcgcg gcattgtcga ttgccatcgc ggtgattggc
    12001 tataacaaaa gttgcaccat tgccaaacca ggcattaaag cgaaagacga gcatatcgcc
    12061 aaaatgatcg ctgaagggaa agtggcgttt ggcctttccg ttgagcacgt cgaacacgcg
    12121 attccgatgt tgattaacca tctgaaataa aaggcacgac tatggatctg tatattcaga
    12181 ttatcgtggt ggcgtgcctg acgggtatga catcgcttct ggcgcatcgc tcggcggctg
    12241 tttttcatga cggcatccac ccgatcctgc cgcaactgat tgaaggctat atgaaccgtc
    12301 gcgaggcggg gagtatcgct tttggtctga gcattggttt tgtggcctcg gtggggatct
    12361 cttttaccct gaaaaccggg ctgctcaacg catggttact ctttcttcct accgatatcc
    12421 tcggcgtact ggcgataaac agcctgatgg cgtttggtct tggcgctatc tggggcgtgt
    12481 tgatccttac ttgcctgttg ccagttaacc agctgctgac cgcgctgccg gtggatgtat
    12541 taggtagcct cggggaatta agctcgccgg tggtttctgc ttttgcactg ttcccgctgg
    12601 tggcgatttt ctaccagttt ggctggaagc aaagtctggt cgccgccgtg gttgttctga
    12661 tgacccgtgt ggtagtcgtg cgctatttcc cacatcttaa ccctgaatcc atcgaaatct
    12721 ttattggcat ggtgatgctg ctggggatcg cgataactca cgacctgcgt catcgtgatg
    12781 aaaatgacat cgatgccaac gggctttcgg tgtttgaaga gcgcacgtcg cggattatca
    12841 aaaacttacc ttatatcgcc atcgtgggag cattgattgc cgccgttgcc agtatgaaga
    12901 tttttgctgg tagcgaagtt tcgatcttca cactggagaa agcgtattcc gcaggcgtga
    12961 cgccggaaca atcgcaaacg ctgattaatc aggcggctct ggcagaattt atgcgcggac
    13021 tggggtttgt gccgttgatt gccaccaccg ccttagcaac gggtgtgtat gcagttgcgg
    13081 gctttacctt tgtttatgcg gtgggctatc tctcgccgaa tccgatggtt gcagcggtat
    13141 taggcgcagt ggttatttcg gcggaagtct tgctgcttcg ttcgatcggc aaatggctgg
    13201 gacgctaccc gtcggtgcgt aatgcgtcgg ataacatccg taacgccatg aatatgctga
    13261 tggaagtggc gctgctggtc ggttcgattt tcgcagcaat taagatggcg ggttataccg
    13321 gattctctat cgcggttgcc atttacttcc tcaacgaatc cctgggccgt ccggtacaga
    13381 aaatggcggc accggtcgtg gcagtaatga tcaccggtat tctgctgaat gttctttact
    13441 ggcttggcct gttcgttccg gcttaaggaa ggcacctatg aagacgtttc ctttgcaaag
    13501 cctgactctt gctgaagcgc agcaaaagca gtttgcgctg gtggacacta tttgccgcca
    13561 ttttccaggc agcgagtttc ttgctggcgg tgatttaggg ttaactccag gtctgaatca
    13621 accgcgtatt acccaacggg tggagcaggt gctggctgat gcatttcacg cacaggccgc
    13681 ggcgctggtg cagggcgcgg ggactggcgc gattcgcgcc gggctggcgg ctttgctcaa
    13741 accggggcag cgtcttctgg tgcatgacgc gcctgtttac ccgacgacac gggttattat
    13801 tgagcagatg gggctgacgc ttattactgc tgatttcaat gacctgtcgg cactgaagca
    13861 ggtcgtcgac gagcaacaac ctgatgccgc gctggtgcag catacgcgcc agcagccgca
    13921 ggacagctat gtgctggcaa cgttgcgcgc gacaggtgtt ccagcgttaa ccgatgataa
    13981 ctatgcggtg atgaaggtag cgcgaatcgg ctgtgaatgc ggcgcgaatg tctcgacatt
    14041 ctcctgcttc aagctgtttg ggccggaggg cgttggtgca gtggttggcg atgccgatgt
    14101 tattagtcgg attcgcgcca cgctttactc cggcggtagc cagatccagg gctcacaggc
    14161 gctggaagtc ttgcgtggtc tggtgttcgc gccagtgatg catgcggtgc aggcgggagt
    14221 atccgaacgg ttgctggctt tgcttaacgg cggcgcggtg gcggaagtga aaagcgccgt
    14281 cattgccaat gcgcagtcga aggtgttgat tgtcgagttt catcagctga ttgccgccag
    14341 ggtgctggaa gaagcgcaaa agcgtggcgc gttgccttac ccggtcggtg cagagtcgaa
    14401 atatgaaatc ccgccgctct tctatcgact ttcaggaacg tttcgccagg cgaatccaca
    14461 actcgaacat tgtgcgattc gcattaaccc gaatcgcagc ggtgaagaga cggtgctgcg
    14521 gattttgcgt gagagtattg cgagtattta atgctggata gcaccgttcg gcaatcaagc
    14581 taaattccgc actgaacggt cccctcgccc ctttggggag agggttaggg tgaggggaac
    14641 gtgcgggatg taaaattcag tgcgccagca tcgccggatg cgatgctggc gcatcttatc
    14701 cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg gcgcggcaga
    14761 agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg ctgatgcgaa
    14821 acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg gcggcaaaga
    14881 tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga aataactgat
    14941 aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac agcgggatcc
    15001 atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag agatggaaca
    15061 cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg acccgttcct
    15121 gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg gtaaaaccac
    15181 tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct tttccctgcc
    15241 agcggatcag tacgccgtct gccactaagt cgctgatggc tttgcgaatg gtaatgcggc
    15301 tgacgttata ttgtgtacaa agctcgtttt cggtagggat ctgttgcccg gtctggtaaa
    15361 ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg agttgatgag
    15421 agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta ctcggattat
    15481 agggtcgttt tccggcaagt aacagcacgt tggcgtgaag atgctcacgc caacgttata
    15541 cctaccaggc accgtggtac tgaatggttt tcgccgcgca cgccgttccc tgcgccatcg
    15601 cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg aacgaatctc
    15661 cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag aactgcgcgc
    15721 catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct gcgccacggg
    15781 caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg gaggcaaagg
    15841 caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg tcggagaagt
    15901 cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc gcatgtcccc
    15961 agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct tcactcaggg
    16021 caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg tcgtgcagtt
    16081 ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata tcgacgccca
    16141 tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc caggtaatgc
    16201 atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca ttaccgccag
    16261 aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt gtcgccaggg
    16321 ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct ggcgggcata
    16381 gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac cctcatagcc
    16441 ccgatcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc caggaatgta
    16501 atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgtaatt tatcgcccag
    16561 tttgtcgaaa taactcatca ccggttccgc ctggacatac ggcgcgcaaa tgtcgaccat
    16621 gctgaacagg cgcggcgaag gcaccagcgc cagcgcatga agcacatcat tagcattaca
    16681 cacgacgttc gattcatacg gcgttaacgg ttcgaggatc agatccatgc cgatgttttc
    16741 cgcgtactca cacagttcgc ttaagttctc tgccagccgc ccccagataa cgttaggcgg
    16801 cgtgagatag cccgcgtggg ccgcagaaat cagcgtataa cccgcgttca tctcttttgc
    16861 catatctatc gccagcttga tcatgtccag gctttcacga cgcatatgtt catcgcccag
    16921 catcatgtta tacggatagc cgttggtttc tggcgtgtaa ccgataatcg gcatctgata
    16981 cgtctgcgcc agcgccttga tttgtttgat gccgcccgct tttaagtccg gcgcgaacgc
    17041 gtgcgggcga ccgccccaaa tttcgatgcc gtcgtaaccc agctcgcttg catcacgaaa
    17101 tgcgtgttca ataggcagcc gctggtggcc gcaggtaaac atacctgttt tcatcgggta
    17161 actccttgcg taaatccggc ccgttgccgg gccgggagag ggattaatat tccaccagac
    17221 caccgtaata gcggcgttca tccgggttgt gatctttgta aatagacagg tagtagcaga
    17281 gccactccat tggcacgaac atcaggaacg gtgccagcca cgggtgcagc ccttgcgaaa
    17341 tttcggcgta atcgatgacg atcacgttgt cagtacgctg tttaacaaag ttaatggcgc
    17401 gttcggtggt gtggcgactt tcatcattgc cgagcaggaa caggaacgga acgcccggtt
    17461 cgacaatctc cagcgggcca tggcggaact ctccgctctc aatcacgcag ccgtgcgtcc
    17521 aggtaaattc catcagcgtt acaatgcctt ctttgtaacc cagcggacgc agcggacccg
    17581 cagcaacggt ataaatcatc ggccactggc tggccagttc accaagctgg cggccttttt
    17641 cttcccaggt gcgtaccaga tgaccgagcg cattcggcaa ctgcttgaga tcgtttttga
    17701 tcttgccgat ttccgcgttc ggcgcgaggc gggtgatcat ctccagcacc acgctgtagc
    17761 agagcagcag gtgaatttcc cagatacagt cggcctgata atcaatgcta aattccgccg
    17821 ccgaggtaat cgggctgtcc gcgcgtttgg tgaacgccgc agtgagtgcg ccgcaggccc
    17881 ggcccagctc cagcgctttg attacctctt ccgttttacc gtagtcagaa acgccaatca
    17941 ccgcgcaacg atcgtcgagg cgatacgggg tgttatcgca gaactcccag ccggaaatgg
    18001 cgtagacctg aagatcggaa aagcgatccg ccagatgttt cgccgtttgc gccgcgttga
    18061 gtggcgagcc gcaggcaacg aaataaatac gatcaatgtc gcgcttcacc atctcttcca
    18121 cgatggcgtg caccagcgga acgtcatggc tgagaacttt ttccacttcc tgaaccatat
    18181 tttcggtgac cagaaagtcc acggtgcttt tatcaatatc caacatttta ttttcttcag
    18241 ataacattac gacaaagcgt tgagctggcg gctacgcttc gcccagaagg cgtaagcagg
    18301 cagaccagta gcaataacga tgacggcgca gataaggccg ggaatcggtg cccagacaaa
    18361 ggttgaggcg accagaatga ggcttgacgc aatggcgagg gtggtcatca gcccgaaagc
    18421 cggagtacgc cacagcggtt tgtaatcgtc gcgtttacga caccagatga tggagccgaa
    18481 ggtgagggta tttttgaaac acatcaccag ggtgaaataa cccagcaggc tggtgagatc
    18541 ggaaacgaag atgaagaaga tccccagcgc cccttgcagg atgatggaga catccggcgt
    18601 gttgtatttc ggatgcacat ggccgaagca tttaaagaac aggttgtctt tcgccatcgc
    18661 gtattccagc cgcggctggt acatcacgca gctggaaagc gaaccaagaa tgacgatcat
    18721 cgccgtgatg gcaacaaaga tcccagcggt gctgccgagt gcggggatct aggtcagggc
    18781 gtcggaaatc ggcgtttcag aattggcgag tttgtcgaag ggcatcaggc cggaaatcac
    18841 cagcgccagc agggtgtaga gcaccagaac cagcagacag gaaccaatca gcgccataaa
    18901 gctgcccgtt gcgccaatcg cagtggtggt aagggcggca aaattctccg ctttaaacca
    18961 gaagatccca aggccaatga cgatagtgaa cgggataatt ttggcgatgg taattagcgt
    19021 ctgaaacgct gcgccgcctt caacggagcg caggtgtagc aacataaagg cgataattaa
    19081 tccggcggcg ataaatttac cgagcaacgg atcgataggc gttaaaaagc caagattgct
    19141 gacaatcgcc agcgccataa tcgacaatga cggcgcatcg ttggcccaga agctggccca
    19201 gccggagagg aaagccagcg gtcggcttcc ggcatttttc agataaacat aatctgcgcc
    19261 attttccgga taagcggtgg atagttccgc atagacgcac atttgcggga tcacaattaa
    19321 cccgccaatg acaaacgcga gcaccgtaag ccacggcgtg cccgctgctt ttgccacttc
    19381 acccacagat acaaaaatac cggagccgac ggttgtcccg acggcgattg caagaacggc
    19441 ccaaaatccg agcttgcgtt ggagttcctg gcttcccata gcgatttttc ttgtccaata
    19501 cgttgtaggg gagagtttgc agtcatttaa aaggtaacat gacaatgcat gatgaatata
    19561 acaacatatg atgttatgta atgtggagga agatcacaaa atttcgcaca ggatcgcgct
    19621 gtggctaatg gatgtagtta tcaaattgaa tttaaagtga aaatattttt acgggcgggg
    19681 gcaagaagga catagaaaca aatacgccct cggaaaatcc agagggcgtc gggcaattaa
    19741 accggtgtta gccgatttct gtcagagact tactgtgcag taggaccgca accgccaatg
    19801 attttagaaa tagaaattgc cgggtgcagc aggtagtcgt aagagcagtt cttatcacgg
    19861 ttttcaatat gaccagtaca tgcagttaac gtagccagta ctgcacctac cagtgcaact
    19921 ttaataaatt tgttcattta cattccctat gtattatata ttaattaata ttcattgatt
    19981 atatgaatat aagcagactg ctcgttaaga acgatacagg aatatttaat tggcgtcagt
    20041 gagcgaatga cttaattttg taagtcgctg taacggtgaa tataaccca
//
LOCUS       DBKEKI010000064        19882 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.064, whole genome shotgun sequence.
ACCESSION   DBKEKI010000064 DBKEKI010000000
VERSION     DBKEKI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.064"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..441
                     /locus_tag="ACUQPX_002349"
     CDS             <1..441
                     /locus_tag="ACUQPX_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HHV9057045.1"
                     /translation="DHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNLTAADGL
                     AVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMAGPQRVC
                     ASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(549..2087)
                     /gene="emrY"
                     /locus_tag="ACUQPX_002350"
     CDS             complement(549..2087)
                     /gene="emrY"
                     /locus_tag="ACUQPX_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HHV9057046.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2087..3250)
                     /gene="emrK"
                     /locus_tag="ACUQPX_002351"
     CDS             complement(2087..3250)
                     /gene="emrK"
                     /locus_tag="ACUQPX_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HHV9057047.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNSQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            3666..4280
                     /gene="evgA"
                     /locus_tag="ACUQPX_002352"
     CDS             3666..4280
                     /gene="evgA"
                     /locus_tag="ACUQPX_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HHV9057048.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            4285..7878
                     /gene="evgS"
                     /locus_tag="ACUQPX_002353"
     CDS             4285..7878
                     /gene="evgS"
                     /locus_tag="ACUQPX_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HHV9057049.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNNYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPSVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFKIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(7934..9079)
                     /gene="yfdE"
                     /locus_tag="ACUQPX_002354"
     CDS             complement(7934..9079)
                     /gene="yfdE"
                     /locus_tag="ACUQPX_002354"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HHV9057050.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9153..10097)
                     /gene="yfdV"
                     /locus_tag="ACUQPX_002355"
     CDS             complement(9153..10097)
                     /gene="yfdV"
                     /locus_tag="ACUQPX_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HHV9057051.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLEPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10167..11861)
                     /gene="oxc"
                     /locus_tag="ACUQPX_002356"
     CDS             complement(10167..11861)
                     /gene="oxc"
                     /locus_tag="ACUQPX_002356"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HHV9057052.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(11915..13165)
                     /gene="frc"
                     /locus_tag="ACUQPX_002357"
     CDS             complement(11915..13165)
                     /gene="frc"
                     /locus_tag="ACUQPX_002357"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HHV9057053.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(13678..14301)
                     /locus_tag="ACUQPX_002358"
     CDS             complement(13678..14301)
                     /locus_tag="ACUQPX_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HHV9057054.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            14598..14873
                     /gene="ypdI"
                     /locus_tag="ACUQPX_002359"
     CDS             14598..14873
                     /gene="ypdI"
                     /locus_tag="ACUQPX_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HHV9057055.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(14950..15192)
                     /locus_tag="ACUQPX_002360"
     CDS             complement(14950..15192)
                     /locus_tag="ACUQPX_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HHV9057056.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            15545..16465
                     /gene="lpxP"
                     /locus_tag="ACUQPX_002361"
     CDS             15545..16465
                     /gene="lpxP"
                     /locus_tag="ACUQPX_002361"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HHV9057057.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTIVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            16821..16892
                     /gene="ypdK"
                     /locus_tag="ACUQPX_002362"
     CDS             16821..16892
                     /gene="ypdK"
                     /locus_tag="ACUQPX_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HHV9057058.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(16957..18195)
                     /gene="alaC"
                     /locus_tag="ACUQPX_002363"
     CDS             complement(16957..18195)
                     /gene="alaC"
                     /locus_tag="ACUQPX_002363"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HHV9057059.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            18572..19843
                     /gene="ypdA"
                     /locus_tag="ACUQPX_002364"
                     /pseudo
     CDS             18572..19843
                     /gene="ypdA"
                     /locus_tag="ACUQPX_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
BASE COUNT         5642 a         4597 c         4229 g         5414 t
ORIGIN      
        1 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
       61 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
      121 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
      181 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
      241 caggaagaag gtattcgtct tgaaccttca gcactggcgg gtatggccgg acctcagcgc
      301 gtgtgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca attacgtaat
      361 gccactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa
      421 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctgggtg
      481 agcgatgctg ccgatggcgc agacttaaga tccccggtct tacccgctat aaccccctcc
      541 ttttatcatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
      601 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
      661 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accatagtat
      721 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggttgctgtc
      781 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
      841 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
      901 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
      961 ccctggaaaa actgtggcaa aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca
     1021 gaacgccagt aatagcaaac cgcatacatc aaaaaactga atgtcactaa cacccgcatg
     1081 tctattttat tgccataacg tcctatcaaa ggtgaaatca atagtggcat gatgccgatg
     1141 ggcgcataag caagcccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     1201 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc aggtgatact cacaatacca
     1261 atggtgaaat tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     1321 tcccaaatga ctaaagagat cagagaaata actgatacta ctgttaatat tattattgta
     1381 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     1441 ccgagcacta acagggtcag tcctggtaga ttcattttta ccggtgaagt ttcagtttct
     1501 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     1561 aaaaatatcc aaccccagct aaagttatcg caaatatagc cgcccaatat cggcccacat
     1621 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggt
     1681 ggataattcc ttaataataa actctgtgac agtggaatta acggccccgc cattaatccc
     1741 tgaacgactc taaaaaatat cagcacatca agattggtcg ataaactaca cattaaggaa
     1801 gacagcgaaa aaaaagtgac tgaaagtaaa aataacctta attcgcctat tctttgtgcc
     1861 agcctgccgg taacagggat cgcaatggca tttgctacgc caaacgaggt gataacccag
     1921 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
     1981 atagtggaat ccaacatttg cataaacgtc gctaatgaca atgcaatagt aacgcaccat
     2041 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
     2101 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
     2161 tactggtata agcaggcatg gaggtcacgg ttgaagccag atcaggcatc tcagcaatgt
     2221 cttcgtcctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
     2281 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
     2341 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
     2401 ctgtcacccg accatgaaac acaacatttt caccataaag atcgctgata atattgactg
     2461 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttggcatta acccacattt
     2521 gacgtgccgg tacgacagcc attaacgatt gtccggaact cactgtttcg ccgacctgaa
     2581 cacttcgctg ggcaatatag ccagtaaccg gactccttat atccgtacgt ttaagcacca
     2641 accaggcttc tttggttgca tccgccgctt caactacttg tggctgactg tttaatggtg
     2701 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc agtgccgctt
     2761 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
     2821 tcgctaaagg cacacgacgg ttataatctt ctaaagattg ttgatactga atacgtgctg
     2881 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
     2941 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
     3001 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaact acagtgacac
     3061 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
     3121 taatcatgtc ttctaattcc attgaccaat aggcataggc acctgaaaac gcaataaata
     3181 aaactatcac caataaagaa aagtattttt ttctgttaga ctgtttttta tttgaattaa
     3241 tctgttccac tattatctct catttcgcat agatgaaatt tatgaattga atgaaaagag
     3301 tattcattct tcatatcaaa cacagtgcat gtgttgcaca ttatcccagg aacgttggag
     3361 gcctaattta aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata
     3421 caattcttac gcctgtagga ttagtgagaa gacttatagt gctaacttga aactataaat
     3481 catcggtata atccctgatt ttattgttga catttcattt atgccgacta tttatatggt
     3541 atacttgtcg aattatctta aaggaagctc agattttctt atttttattg agaaaatgag
     3601 atgacgcctt atgtctgtat tactacaggg agaagggaga tgcttcattg caaagggaat
     3661 aatctatgaa cgcaataatt attgatgacc atcctcttgc tatcgcagca attcgtaatt
     3721 tattgatcaa aaacgatatt gaaatcttag cggagttaac tgaaggcgga agcgccgttc
     3781 agcgggtgga aacacttaag cctgatatcg tcatcattga tgtcgatatc cccggagtta
     3841 acggtatcca ggtgttagaa acgctgagga agcgccaata tagcggaatt attattatcg
     3901 tctccgctaa aaatgaccat ttttacggga aacattgtgc tgatgctggc gctaatggtt
     3961 tcgtgagtaa aaaagaaggc atgaacaata tcattgcggc tattgaagct gcaaaaaatg
     4021 gctactgcta tttccccttc tctctcaacc ggtttgttgg aagtttaacg tccgaccagc
     4081 aaaaactcga ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt cttgatggca
     4141 aggataataa tgacattgcg gaaaaaatgt tcatcagcaa caaaactgtc agcacgtata
     4201 aaagtcgcct gatggaaaaa ttagaatgta aatcactgat ggatctttac acattcgcgc
     4261 aacgtaacaa aatcggctaa tcacatgaag tttttaccct atttttttct tctctgttgt
     4321 ggtctttggt cgaccataag tttcgcagac gaagattaca tcgaatatcg tggcatcagt
     4381 agtaacaacc gtgtcacact tgatccagta cgtctgagca acaaggaatt acgttggtta
     4441 gcgagcaaaa aaaatcttgt gattgcagta cacaagtccc aaacggctac cttgttgcat
     4501 accgattcgc agcaacaggt tcgtggtatt aatgctgatt atttaaatct tttaaaaaga
     4561 gcgttaaata tcaaattaac actccgggaa tacgcagatc atcaaaaagc aatggacgcg
     4621 cttgcagaag gtgaagtcga tatagtgtta tcacatttag ttacttcgcc gcctcttaat
     4681 aatgacattg ctgcaaccaa accattgata attacctttc cggcgctggt aaccaccctt
     4741 cacgactcaa tgcgaccgct tacctcacca aaaccagtaa atattgctcg ggtagcaaat
     4801 taccccccag acgaggtaat tcatcaatca tttccaaaag caacaattat ctcttttaca
     4861 aatttatatc aggcattagc atccgtctca gctgggcaca ataattactt tattggtagt
     4921 aacatcatta ccagcagtat gatttcccgc tatttcactc actccttaaa tgtagtgaaa
     4981 tattataact cgccgcgtca atataatttt ttcttgacca gaaaagaatc tgtcattctt
     5041 aatgaagtac tcaatagatt tgttgatgct ttaacaaatg aagttcgcta tgaagtatca
     5101 caaaattggc ttgatacagg aaacctagcc tttctgaaca aaccattaga actcactgaa
     5161 catgaaaaac agtggattaa gcagcatccc aatttaaagg tgctggaaaa tccttactcg
     5221 cccccctatt ctatgacgga tgaaaatggc tcggttcggg gcgttatggg ggacattctt
     5281 aatattatta ccttgcaaac aggtttaaat ttttctccga tcaccgtttc acacaatatc
     5341 catgctggaa cacagcttag ccccggagga tgggatataa tacctggcgc tatttatagt
     5401 gaagatcgag aaaataatgt tttatttgct gaagccttca taacaacgcc ttacgttttt
     5461 gtcatgcaaa aagcgcctga cagtgaacaa acattaaaaa aaggaatgaa agttgccatt
     5521 ccatattatt atgagctgca ttcgcaatta aaagagatgt atccggaggt tgaatggata
     5581 caggtcgata atgccagcgc tgcatttcac aaggttaagg aaggtgaact tgatgctctg
     5641 gtcgcgacac agctaaattc gcgttacatg atcgatcatt actatcctaa tgaactttat
     5701 cattttctta ttcctagcgt tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa
     5761 ccggaactta aggatattat taataaagca ctgaatgcaa ttcccccaag cgaagttctg
     5821 cgcctgacgg aaaaatggat taaaatgccc aatgtgacca ttgacacatg ggacctatat
     5881 agcgagcaat tttatattgt tacgacatta tccgttttat tagttggcag tagcctttta
     5941 tggggattct acctgttacg ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa
     6001 aaccaaatat cattccgaaa agcactctcg gattccttac cgaatccaac ttatgttgta
     6061 aactggcaag gtaatgtcat tagtcataat agtgcttttg aacattattt cactgcggat
     6121 tactacaaaa atgcaatgtt accattagaa aacagtgact caccctttaa agatgttttt
     6181 tctaatgcgc atgaagtcac agcagaaacg aaagaaaatc gaacaatata cacacaggta
     6241 tttaaaattg ataatggcat cgagaaaaga tgcattaatc actggcatac attatgcaat
     6301 cttcctgcaa gtgacaatgc agtatatatt tgtggttggc aagatattac tgaaacgcgt
     6361 gatctaatta atgcactcga ggtagaaaaa aataaagcga taaaggctac cgtagcaaaa
     6421 agtcagtttc tggcaacgat gagtcacgaa ataagaacac caataagctc tattatgggc
     6481 ttcctggaac ttctgtcggg ttctggtctt agcaaggagc aacgggtgga ggcgatttca
     6541 cttgcctacg ccaccggaca atcactcctc ggcttaattg gtgaaatcct tgatgtcgac
     6601 aaaattgaat cgggtaacta tcaacttcaa ccacaatggg tcgatatccc tactttagtc
     6661 cagaacactt gtcactcttt cggtgcgatt gctgcaagca aatcgatcgc attaagttgc
     6721 agcagtacat ttcctgatca ttacctggtt aagatcgacc ctcaggcgtt taagcaggtc
     6781 ttatcaaatt tactgagtaa tgctctcaaa tttaccaccg agggggcagt aaaaattacg
     6841 acctccctgg gtcacattga tgacaaccac gctgttatca aaatgacgat tatggattct
     6901 ggaagtggat tatcgcagga agaacaacaa caactgttta aacgctacag ccaaacaagt
     6961 gcaggtcgtc agcaaacagg ttctggttta ggcttaatga tctgcaaaga attaattaaa
     7021 aatatgcagg gcgatttatc attagaaagt catccaggca taggaacaac atttacgatc
     7081 acaatcccgg tagaaattag ccagcaagtg gcgactgtcg aggcaaaagc agaacaaccc
     7141 atcacactac ctgaaaagtt gagcatatta atcgcggatg atcatccgac caacaggcta
     7201 ttactcaaac gccagctaaa tctattagga tatgatgttg atgaagccac tgatggtgtg
     7261 caagcgctac acaaagtcag tatgcaacat tatgatctgc ttattactga cgttaatatg
     7321 ccgaatatgg atggttttga gttgactcgc aaactccgtg agcaaaattc ttccttaccc
     7381 atctgggggc ttacagccaa cgcacaggct aacgaacgtg aaaaagggtt aagttgcggc
     7441 atgaacttat gtttgttcaa accgttgacc ctggatgtac tgaaaacaca tttaagtcag
     7501 ttacaccagg ttgcgcatat tgcacctcag tatcgccacc ttgatatcga agccctgaaa
     7561 aataatacgg cgaatgatct acaactgatg caggagattc tcatgacttt ccagcatgaa
     7621 acgcataaag atctacccgc tgcgtttcat gcactagaag ctggcgataa cagaactttc
     7681 catcagtgta ttcatcgcat ccacggtgcg gctaacatcc tgaatttgca aaagttgatt
     7741 aatattagcc atcagttaga aataacacct gcttcagatg acagtaagcc tgaaattctt
     7801 cagctgctga actctgttaa agaacacatt gcagagctgg accaggagat tgctgttttc
     7861 tgtcagaaaa atgactaaat tgcggctccc acaatgttct aatgtgggag ctatttacca
     7921 gcacatctta catttatgat gagaactcct ggcgaatttg ttccccatgc tggtcgagcg
     7981 ttgccgctcc cggcataaca tgcgggtccg cgcagccgct gatttttatc ggattacccg
     8041 gcatcattat tcctccggct tcaatcaaca tatttctcgc ctgagtttgt ggcaaattaa
     8101 tggcctcagc cacacttaat aacggcgcga cgggcacacc aacttcatgt attttggcta
     8161 accaaacttc agctgcctgc gtttttaacg ttcgctcaat atattgttta agaatagcct
     8221 ggttttgtac gcgtaaaata ttgctgctaa atcggggatc attaaccagt tccgtaagct
     8281 ccagtgcctg gcataacgca gaaaaaagct tgtcattacc acaacaaatc gtaatcggct
     8341 tatcctgagt atcgaaaaca tcaaaaggtg ccatgtaggg atggcgattt cccaggcgtt
     8401 gtggtgactt ccctgtcgca atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg
     8461 catcaaacat cgctatatcg acatgcgccc ctctctggct cttttcgcgg ccataaagtg
     8521 cactcactat tccgctgaat aaatagacac cgccgcatag atccgcaaga gaggtaccaa
     8581 cacgcactgg cggagcatca gggtaccctg tttccatcat tatcccactc attgcctgaa
     8641 tgatggtatc gtaggcagga gcatctttta gcggaccggt atgtccgaaa cctgacgatg
     8701 aagcatatat gaggcgcggg ttgatttctt gtaacctttc ccatgaaaac cccagttttt
     8761 ccattgtacc tgggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
     8821 atatactttt atcatgatca ttctttaaat caagaaccac actctctttg ccatgattaa
     8881 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
     8941 catgacctgg tggctcaact ttaattaccc ttgcgcccat attataaaga agttgagttc
     9001 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
     9061 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
     9121 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
     9181 cacaagggag ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta
     9241 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
     9301 cctgccagta ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga
     9361 agcagcgcca gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg
     9421 aactcgaatt tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta
     9481 gccttcgcaa tcagattaaa ggttgggtcc catgctgccg gaatttttac cccaaccagc
     9541 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
     9601 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
     9661 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
     9721 gatacggaat caccataaat aggctcgaga actgcaaacc ccaggaatcc aatggtaggt
     9781 gaacccgcaa ttaatgcaca tacagctgct tctgcatggg tacgtttaaa aaatttgtag
     9841 caaccaaacc aggagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
     9901 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
     9961 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
    10021 tctcgtctgc cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
    10081 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
    10141 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
    10201 gtaatatggc cactttcagt tcctgctgca gggtcgatga ccacattaat aatggtcggt
    10261 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
    10321 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
    10381 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
    10441 ttattaaaaa taacgatcgt caccggcagg ttatatcgac aaatcgtttc aatttccatc
    10501 ccactgaaac caaaagcact atcaccttca atggcgacaa ccggagaacc agaagtcacg
    10561 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
    10621 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccaaggt atttgcacct
    10681 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
    10741 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
    10801 tgctgcttgt ggatatttaa aatatctcgc cataccagtg gagtcgtaaa tgtgttttgt
    10861 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
    10921 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
    10981 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
    11041 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
    11101 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata
    11161 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
    11221 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
    11281 aatgctggcg acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
    11341 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
    11401 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
    11461 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
    11521 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
    11581 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
    11641 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
    11701 tcatgacgaa aaccaatata acgaatgcct tccgcctggg catggcgtgc catatccgtc
    11761 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
    11821 ataatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
    11881 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
    11941 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
    12001 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
    12061 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
    12121 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
    12181 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
    12241 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgga tcggtaatcc
    12301 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
    12361 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
    12421 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
    12481 gcgggtattc ttccagataa cccaatttat cgagacgctg ctggtcacgt aatttcacgc
    12541 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
    12601 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
    12661 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
    12721 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
    12781 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
    12841 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
    12901 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
    12961 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
    13021 gttggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
    13081 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
    13141 taattccttg aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat
    13201 ttagcgttta tttaaggcat aacgccctct ataaataatc tgcaaaatat cttcgtggcg
    13261 gcactcgata tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gccacttcca
    13321 attcctttct gcatggtaag tagtgaggac gaaaagaaga atatgataaa tattgattcg
    13381 tggtaatcag agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg
    13441 gtgatttaca agtaagagta tgattacgct ttttaaagcg taccataaag cataacataa
    13501 taaatgattt gtgatgacac gggattattg cggtttactt tgaggaaaat aaaatgcgtg
    13561 tttatagaac cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata
    13621 aaaaattgtc ggctggaggg agttaaatcc tttacctcaa tatactgcct gtcagcatta
    13681 gttcacaaac agtgcttcgc tatcgacgat gattccatct tccgcacctt taagcgccag
    13741 gtttgcctcg tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg
    13801 agtctgttta agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag
    13861 ctcttccatt gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc
    13921 ggcttctttt tcgggtgttg ctacataact ctcagaaatt ccgacagatg cattaatgac
    13981 aatatattgg tcatcattga gattggtctt tttacctggc ttagcgaact ttgcccattc
    14041 ggtactgtca tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttccgg
    14101 atcgccatga aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg
    14161 ttcagagata cgctctgctg ctgccgtttt ctgggtttgc tgagtttgct gttgtgccgc
    14221 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
    14281 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
    14341 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat
    14401 cttacaattg tagttattat cgcaaagcgg catttagtct tacagttgta ggaatatgct
    14461 tcctgtcgca gagaactatt cttatatacc acaaagagat gtcatttatt atattgatgt
    14521 aaatattgat taattatatg taaatacagc cataacatcg tatggttctc agtgaaccat
    14581 tttcaggata actttctatg aaagtaaact taatactttt cagcttattt ttattggtct
    14641 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct
    14701 ataaagagac tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa cttcagccat
    14761 cacaggcgat tttgtttaag atgagagaag atgttccacc attaaacctc acagaagaaa
    14821 tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgcga tagcgtctgt
    14881 cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat
    14941 cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
    15001 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
    15061 gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac
    15121 gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca
    15181 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
    15241 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt
    15301 taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc
    15361 tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt
    15421 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
    15481 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
    15541 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
    15601 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
    15661 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc
    15721 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat
    15781 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg
    15841 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc
    15901 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
    15961 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
    16021 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag
    16081 aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc
    16141 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
    16201 cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt
    16261 aacgatagta agaaaagcgg attacagcgg atatcgtttg ttcatcaccc cagagatgga
    16321 aggctacccg acagatgaaa atcaagccgc tgcctatatg aacaagatta tcgagaaaga
    16381 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
    16441 gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc
    16501 cataataacc tcatgatttt tatggctttt tttcttaagt tttttccttc gctgaatatc
    16561 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    16621 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    16681 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    16741 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    16801 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggttatcg
    16861 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    16921 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    16981 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    17041 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    17101 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    17161 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    17221 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    17281 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    17341 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    17401 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    17461 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    17521 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    17581 cacatcgtag cgtttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    17641 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    17701 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    17761 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    17821 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    17881 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    17941 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    18001 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    18061 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    18121 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    18181 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    18241 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    18301 gaacattttt atcaatggct ttaaatgagg aactccacgt caaaaacagg aaatttttct
    18361 acgctaacaa gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag
    18421 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    18481 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    18541 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    18601 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    18661 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    18721 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    18781 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    18841 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    18901 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    18961 gaaaatcccc aaagcacagc gctggcgcgt cggtattctc ggcggcatgt tgtgtgagac
    19021 gttgaccatg attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt
    19081 ctctaaaatc ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgcttct
    19141 ggtgcaaagc gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca agctggcgct
    19201 ggatatcgcc aacaaaacgc taccgctgtt tcgccatgtc aatagcgagt cattacgcaa
    19261 ggtctgcgaa attatccgcg atgacattca cgccgatgcg gtggcgatta ctaataccga
    19321 tcatgtgctg gcctatgttg gcgtgggtga acataactat cagaatggcg atgacttcat
    19381 tagcccgact acccgtcagg cgatgaatta cggaaaaatc atcattaaaa acaatgatga
    19441 agcccaccgc acaccagaga ttcattccat gctggtgatc ccattgtggg agaaaggggt
    19501 cgtgaccgga acgctgaaaa tttactactg ccacgcgcat cagatcacct cgtcattaca
    19561 ggaaatggcg gtcggtctgt cgcaaatcat ctccacgcaa ctggaggttt cacgcgccga
    19621 gcagctacgt gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc
    19681 ccattttctg tttaacgctc tgaacgctat ttcatcgtca atccgtctga atccggatac
    19741 cgctcgccag ttgatcttta atctgtcgcg ttatctgcgc tataacattg aattaaaaga
    19801 cgatgagcaa atcgatatca aaaaagagct gtatcaaatt aaaggtaatg ctgccaactt
    19861 actgatttag tgtatgatgg tg
//
LOCUS       DBKEKI010000065        18895 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.065, whole genome shotgun sequence.
ACCESSION   DBKEKI010000065 DBKEKI010000000
VERSION     DBKEKI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.065"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1025)
                     /locus_tag="ACUQPX_002365"
     CDS             complement(<1..1025)
                     /locus_tag="ACUQPX_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HHV9057060.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKV"
     gene            complement(1122..1598)
                     /gene="greB"
                     /locus_tag="ACUQPX_002366"
     CDS             complement(1122..1598)
                     /gene="greB"
                     /locus_tag="ACUQPX_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HHV9057061.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            1826..2545
                     /gene="ompR"
                     /locus_tag="ACUQPX_002367"
     CDS             1826..2545
                     /gene="ompR"
                     /locus_tag="ACUQPX_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HHV9057062.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            2542..3894
                     /gene="envZ"
                     /locus_tag="ACUQPX_002368"
     CDS             2542..3894
                     /gene="envZ"
                     /locus_tag="ACUQPX_002368"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HHV9057063.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(3970..5592)
                     /gene="pckA"
                     /locus_tag="ACUQPX_002369"
     CDS             complement(3970..5592)
                     /gene="pckA"
                     /locus_tag="ACUQPX_002369"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HHV9057064.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            5971..7695
                     /locus_tag="ACUQPX_002370"
     CDS             5971..7695
                     /locus_tag="ACUQPX_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HHV9057065.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWALWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(7830..8708)
                     /gene="hslO"
                     /locus_tag="ACUQPX_002371"
     CDS             complement(7830..8708)
                     /gene="hslO"
                     /locus_tag="ACUQPX_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HHV9057066.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEMPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(8733..9134)
                     /gene="hslR"
                     /locus_tag="ACUQPX_002372"
     CDS             complement(8733..9134)
                     /gene="hslR"
                     /locus_tag="ACUQPX_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HHV9057067.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALARKMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(9145..9813)
                     /gene="yrfG"
                     /locus_tag="ACUQPX_002373"
     CDS             complement(9145..9813)
                     /gene="yrfG"
                     /locus_tag="ACUQPX_002373"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HHV9057068.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWSAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(9878..12013)
                     /gene="igaA"
                     /locus_tag="ACUQPX_002374"
     CDS             complement(9878..12013)
                     /gene="igaA"
                     /locus_tag="ACUQPX_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HHV9057069.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPSPTGASAAPLSLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATGVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            12333..12893
                     /gene="nudE"
                     /locus_tag="ACUQPX_002375"
     CDS             12333..12893
                     /gene="nudE"
                     /locus_tag="ACUQPX_002375"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HHV9057070.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFYEARNVSALFLVREWLKGQGRV"
     gene            complement(13058..15610)
                     /gene="mrcA"
                     /locus_tag="ACUQPX_002376"
     CDS             complement(13058..15610)
                     /gene="mrcA"
                     /locus_tag="ACUQPX_002376"
                     /EC_number="2.4.99.28"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HHV9057071.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITHKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLGQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            15730..16509
                     /gene="hofM"
                     /locus_tag="ACUQPX_002377"
     CDS             15730..16509
                     /gene="hofM"
                     /locus_tag="ACUQPX_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HHV9057072.1"
                     /translation="MAFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            16509..17048
                     /gene="hofN"
                     /locus_tag="ACUQPX_002378"
     CDS             16509..17048
                     /gene="hofN"
                     /locus_tag="ACUQPX_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HHV9057073.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            17032..17472
                     /gene="hofO"
                     /locus_tag="ACUQPX_002379"
     CDS             17032..17472
                     /gene="hofO"
                     /locus_tag="ACUQPX_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HHV9057074.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            17462..17866
                     /gene="hofP"
                     /locus_tag="ACUQPX_002380"
     CDS             17462..17866
                     /gene="hofP"
                     /locus_tag="ACUQPX_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HHV9057075.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNYVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            17778..>18895
                     /gene="hofQ"
                     /locus_tag="ACUQPX_002381"
     CDS             17778..>18895
                     /gene="hofQ"
                     /locus_tag="ACUQPX_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HHV9057076.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQ"
BASE COUNT         4315 a         4874 c         5135 g         4571 t
ORIGIN      
        1 acttttaccc cggtacgcag acccggatcg aggcccatcg ttgcacgcag tccggcaggg
       61 gccgccatca gcagatcgtg caggttacgg gcaaagacgt tgattgcttc atcttccgcg
      121 cgttcgcgca cggtacccat cagttcggtt tccagatgca tcagcacctt gatgcgccag
      181 gtccagctca ctacgccttt gcgccagcta tccgccgggg cattgttcag gcgcaggcca
      241 aggtgatcca tgatgatttg ctcgcaatag ctctctttgg gcggctcatc gaactgcgga
      301 tcggcattca gcgaaagctg gagtacgcct tcgttacgcc cacggaacat cgccagcgcg
      361 cggtgagaag gcaccgtgga caacggttcg tgatgatcga aatagtcgcg gaatttcgcc
      421 ccttcctctt ctttaccgct caccaccgta gaaaccaaat gcgcgttctt ccacagataa
      481 tcacgcactt tcgccagcag cgcggcatct tcggcaaacc gttccatcag gatatagcgc
      541 gcgccatcca gcgcggcttt ggtatctgcc acgcctttat cggcatcaac atattgtgca
      601 gcggcgactt ctggcgtgtg tgacggatcg ctccacagca ggtcagccaa cggctcaagc
      661 cctgcttcaa tggcgatttg cccgcgggtg cggcgtttag gtttgtaggg caggtagagg
      721 tcttcgagtt cggttttgct tagggtggcg ttgatggcct tcgccagatc atcggtgagt
      781 ttgccttgct cggaaatgga tttgaggatc gcctgacgtc tctcttccag ctcgcgcaga
      841 tagctcagac gcgtttccag attacgcagc tgcgtgtcat ccagaccgcc ggtgatttcc
      901 ttacgataac gtgcgataaa cggcacggta ttcccttcgt caagcaggcg aacggcagcg
      961 tcaacctgtt ccgggcgcgc ctgaatttca cccgcaataa tgcggcagaa cgaatcattc
     1021 atcatggttt gagttcatct tttcggatca aaaatcaggg gatagttata cggactggct
     1081 ggcaaaaatg ccagccatcg gcaggaggtt aagactcttc cttacggttt cacgtactcg
     1141 atagcattaa cataccagtt cgcttccccg gcaggggtat tcaccaccgc cagatcgccg
     1201 acttcttttt tcagcaatgc gcgggccatc ggggaatcga tagagatgta atctttacgg
     1261 ccaaaaattt catcgtagcc gacaatacgg aaacggtgag tcacgccatc gtcgttttca
     1321 atctccaccc acgcgccaaa aaagactttg ccttcctgct gaggggagta atcgacgatt
     1381 ttgagatttt ccaggcattt agtgagatag cgcacgcgac ggtcgatttc acgcagacgc
     1441 tttttattat actgatagtc agcattttcg ctgcggtcgc ccagacttgc ggcccaggtc
     1501 accttttttg tgacctccgg gcgttcttca cgccagagat aattaagctc ttgtttgagt
     1561 ttttcatacc cttcccgggt aaccaggggc gttttcatct cgttgattcc ctttgtctgt
     1621 ttgataatgc gcacattggg tataacgtga tcatatcaac agaatcaata atgtttcgcc
     1681 gaataaattg tatacttaag ctgctgttta atatgctttg taacaattta ggctgaaatt
     1741 cataccagat ttagctggtg acgaacgtga gcttttttaa gaatacacgc ttacaaattg
     1801 ttgcgaacct ttgggagtac aaacaatgca agagaactac aagattctgg tggtcgatga
     1861 cgacatgcgt ctgcgtgcgc tgctggaacg ttatctcacc gaacaaggct tccaggttcg
     1921 aagcgtcgct aatgcagaac agatggatcg cctgctgact cgtgaatctt tccatcttat
     1981 ggtactggat ttaatgttac ctggtgaaga tggcttgtcg atttgccgac gtcttcgtag
     2041 tcagagcaac ccgatgccga tcattatggt gacggcgaaa ggggaagaag tggaccgtat
     2101 cgtaggcctg gagattggcg ctgacgacta cattccaaaa ccgtttaacc cgcgtgaact
     2161 gctggcccgt attcgtgcgg tgctgcgtcg tcaggcgaac gaactgccag gcgcaccgtc
     2221 acaggaagag gcggtaattg ctttcggtaa gttcaaactt aacctcggta cgcgcgaaat
     2281 gttccgcgaa gatgagccga tgccgctcac cagcggtgag tttgcggtac tgaaggcact
     2341 ggtcagccat ccgcgtgagc cgctctcccg cgataagctg atgaaccttg cccgtggtcg
     2401 tgaatattcc gcaatggaac gctccatcga cgtgcagatt tcgcgtctgc gccgcatggt
     2461 agaagaagat ccagcgcatc cgcgttacat tcagaccgtt tggggcctgg gctacgtctt
     2521 tgtaccggac ggctctaaag catgaggcga ttgcgcttct cgccacgaag ttcatttgcc
     2581 cgtacgttat tgctcatcgt caccttgctg ttcgccagcc tggtgacgac ttatctggtg
     2641 gtgctgaact tcgcgatttt gccgagcctc cagcagttta ataaagtcct cgcgtacgaa
     2701 gtgcgtatgt tgatgaccga caaactgcaa ctggaggacg gcacgcagtt ggttgtgcct
     2761 cccgctttcc gtcgggagat ctaccgtgag ctggggatct ctctctactc caacgaggct
     2821 gccgaagagg caggtctgcg ttgggcgcaa cactatgaat tcttaagcca tcagatggca
     2881 cagcaactgg gcggcccgac ggaagtgcgc gttgaggtca acaaaagttc gcctgtcgtc
     2941 tggctgaaaa cctggctgtc gcccaatatc tgggtacgcg tgccgctgac cgaaattcat
     3001 cagggcgatt tctctccgct gttccgctat acgctggcga ttatgctatt ggcgataggc
     3061 ggggcgtggc tgtttattcg tatccagaac cgaccgttgg tcgatctcga acacgcagcc
     3121 ttgcaggttg gtaaagggat tattccgccg ccgctgcgtg aatatggcgc ttcggaggtg
     3181 cgttccgtta cccgtgcctt taaccatatg gcggctggtg ttaagcaact ggcggatgac
     3241 cgcacgctgc tgatggcggg ggtaagtcac gacttgcgca cgccgctgac gcgtattcgc
     3301 ctggcgactg agatgatgag cgagcaggat ggctatctgg cagaatcgat caataaagat
     3361 atcgaagagt gcaacgccat cattgagcag tttatcgact acctgcgcac cgggcaggag
     3421 atgccgatgg aaatggcgga tcttaatgca gtgctcggtg aggtgattgc tgccgaaagt
     3481 ggctatgagc gggaaattga aaccgcgctt taccccggca gcattgaagt gaaaatgcac
     3541 ccgctgtcga tcaaacgcgc ggtggcgaat atggtggtca acgccgcccg ttatggcaat
     3601 ggctggatca aagtcagcag cggaacggag ccgaatcgcg cctggttcca ggtggaagat
     3661 gacggtccgg gaattgcgcc ggaacaacgt aagcacctgt tccagccgtt tgtccgcggc
     3721 gacagtgcgc gcaccattag cggcacggga ttagggctgg caattgtgca gcgtatcgtg
     3781 gataaccata acgggatgct ggagcttggc accagcgagc ggggcgggct ttccattcgc
     3841 gcctggctgc cagtgccggt aacgcgggcg cagggcacga caaaagaagg gtaaataaac
     3901 gggaggcgaa ggtgcctccc gttttgcttt ctataagata ctggatagat attctccagc
     3961 ttcaaatcat tacagtttcg gaccagccgc taccagcgcg gcacccgcag gggtgtcggt
     4021 gtatttatcg aagttgtcga taaacagttt cgccagggtt tcggcttttt cctgccactg
     4081 ttccggagaa gcgtaggtgt tacgcggatc gagaatcttc gtgtctacgc ccggcagttc
     4141 ggttgggatc gccaggttaa acatcggcag agtgaaggtt tctgcattat ccagcgaacc
     4201 gttgaggatg gcgtcgataa tggcgcgggt atctttaatc gagatacgtt tgccagtgcc
     4261 gttccagcca gtgttaacca gataagcctg cgcgcccgcc gcctgcatac gtttcaccag
     4321 cacttctgcg tactgagtcg ggtgcagcga caggaacgcc gcgccgaagc aagcggagaa
     4381 ggttggcgtc ggttcggtga tgccacgctc agtaccggcc agtttggcgg tgaagccaga
     4441 gaggaagtga tactgggttt gatcggcagt caggcgagaa accggcggca acacgccgaa
     4501 agcatcagca gtcaggaaga taaccttagt cgcgtggccc gctttggaaa ccggcttaac
     4561 gatgttatcg atgtgataga ttggataaga aacgcgggtg ttctcggttt ttgaaccatc
     4621 atcaaagtcg atagtgccat cttcacgcac ggtgacgttt tccagcagtg catcacgacg
     4681 gatagcgttg tagatttcag gttccgcttc tttcgacagc ttgatagttt ttgcgtagca
     4741 gccgccttcg aaattaaaca cgccgtcatc gtcccagccg tgttcgtcat cgccaatcag
     4801 gcggcgtttc gggtcggtgg aaagggtggt tttaccggtg ccggaaaggc cgaagaacac
     4861 cgcaacatcg cctttctcgc caacgttggc ggagcagtgc atagaagcga tacctttcag
     4921 cggcagcagg tagttcatca tcgagaacat ccctttcttc atttcgccgc cgtaccaagt
     4981 gccgccaatc agctgcatgc gctcggtcag gttaaacgcc acgaagtttt cggagttcag
     5041 accctgttct ttccactgcg ggttagtgca cttcgcgccg ttcataacga taaagtctgg
     5101 tttgaaacct gccagctctt catcgctcgg gcgaataaac atgtttttga caaaatgcgc
     5161 ctgccaggcc acttcggtga tgaaacggac ggaaagacga gtatccgggt tcgcaccaca
     5221 gaaagcgtcg acaacgaaca gacgtttgcc ggaaagctgc ctggtcacca ggcctttcag
     5281 atgctgccag gtttccggag agagaggttt gttgtcgttc ttacctttgc ctttgtctgc
     5341 ccaccagaaa gtatcgcgag tggtatcgtc acggacgata tacttatctt ttggtgaacg
     5401 accggtgaag atcccggtat cgacggcaac ggcacccaga ttagttaaca ccccgcgctc
     5461 ataacctgtc aggctcggat cgagctcttc ctgatacagc aggtcgtagc ttgggttgta
     5521 aacgatatca tgtacgtcac tgataccata agcctcgagt tcttgcgggg tcaaaccatt
     5581 gttaacgcgc atttcactgc tccttagcca atatgtattg cctgaatagt aaagtctttt
     5641 tgggggtgtt aaccgcgaca aggctcatag atttacgtat ctggagaaat tcatatttga
     5701 caaaaaactc cgtgattcct gtcacgaaac ggttgctatt atcgcagaaa tagcatttta
     5761 ggtgtggaaa atgttctcaa aaggttaaat cttggtttat atggcgggaa tattgtgagt
     5821 ggaatcgcat tcctgaatga aattgatgca aattcaaccg gtaaagtatc gattcaccac
     5881 gctaactttt tttgattatt ttctgaaaaa agcaaaatac tactgcccgg atgcgataat
     5941 taacccacct tttgattctg gataaccaac atggataacg ttgaactttc accggcaaca
     6001 cgctggggga tgattgctac cggattactt caggggctgg tttgctacct gcttatcgcc
     6061 tggcttgccg gaaaaaatca cagttggatt gtttatggcg tgcctgcgac cgtcgcgttt
     6121 tcatccgttt tactcttctc cgtgatctct tttaaacaga agcgcctttg gggatggctg
     6181 gcgctggtgt ttattgccac gacagggatg agcggttggc tgaagtggca aactgatggc
     6241 atgaacccct ggagagctga aaaggcgatt tgggattttg gctgctatct gctgttgatg
     6301 gcaatgttgt tgctaccgtg gatacaacaa agccttcgta tccgcaatgg cagtagccgc
     6361 tatagctact tttaccagtc agtatggcat aacgtactta tattactggt gatttttctc
     6421 tccaatggcc tgacgtggct ggtgctttta ctgtggggtg agttgtttaa acttgttggc
     6481 atcacgtttt tcaatacgct cttttttgca accgactggt ttatgtatct tacatttggt
     6541 ctggttaccg cactggcggt gatcctcgcg cgtacacagt cacgtttaat cgactctatt
     6601 caaaagttgt tcacgctaat cgccacgggg ttgctgccgt tagtatcatt gctaaccctg
     6661 atgtttatca tcaccctgcc gtttacgggc ctgagcgcga tttctcgcca catctccgcc
     6721 gccgggttgc tgttaacgct ggcctttttg caattgatct taatggctat tgtccgcgat
     6781 ccgcaaaaag cgtcacttcc ctggacgggg ccgttgcgtt gcctgattaa aaccgctttg
     6841 ctggttgctc cgctgtatgt gttcgtcgcc acctgggcgt tatggctgcg ggtcgctcag
     6901 tacggctgga ctgtcgaccg cttgcagggc gcgctggcgg agctggtgtt actggtgtgg
     6961 tcgctggggt attttgtcag tatcgtctgg cgtaatgggc aaaatcccct tgttcttcag
     7021 ggcaaagtga accttgcggt ttcgttattg gtgttggtga tactggtgct tcttaattcg
     7081 ccggtgctgg acagtatgcg tattagcgtg aacagccata tggcgcgtta tcagagcggc
     7141 aaaaacacgc cagaccaggt aactatctac atgctcgaac agagcggtcg ctatggacgt
     7201 gcggcgcttg agtcgctgaa aagcgatgcc gggtttatga aagacccgaa acgcgcgcgg
     7261 gatctgctga tggcgttaga tggagagcaa catcttcagg aacaggtatc ggaaaaagta
     7321 ttagccgaaa atgtgttaat tgcccctggt tctgttaaac cggatgcgac attctggtcg
     7381 gccttaatcc aggatcgcta taacgtgatg acctgtattg aaaaagacgc ctgcgtactg
     7441 gtcgagcaag atctgaatag tgatggtcag gcggagcgga tcctgtttgc ttttaatgat
     7501 gacagagtca ttgtctatgg ctttgactca gacagaaaag aatgggacgc gcttgatatg
     7561 agtttacttc cgaacgaaat aacgaaagaa aaattactca cagctgcgaa ggatggaaaa
     7621 ctggggacga ggccgaaagc gtggcgcgat ctgacggtag atggtgaaac gctggaaata
     7681 aacttaagta agtgacaacg cttttatcgg atacgacgct gtatctgacc aaaataattt
     7741 gcgatttatt tcataggcgc tggtaagcgc agcacatcag gccattaagt cgtaaaatcc
     7801 ggcagagccc tctctgccgg acatactcat taatgaactt gcggatctgc cggagacgcg
     7861 ttgttgcgga tttcagcaat atccatcgca ttgaacagat agtggttacc gcagtaatca
     7921 caatgcatgt caatttcgcc atcttccgcc aggatgctat caacttcttc atcaggcagc
     7981 gttttcagcg catcggcgca acgttcacgc gagcaggtgc atttgaactc cacatcctgc
     8041 ggatcgtaaa ccgtcacctc ttcttcgtga tacaaacgcc acaacacttc gtttgccggt
     8101 aaggtcagca gttcttcggt tttgatggtt tcggttagcg tcgccaggtg gtcaaagtcg
     8161 tcctgctggg tattttgcgc aggcattacc tgcaacaaca taccgcctgc agccggtttg
     8221 ccgtctacgt cgccggtgcg aataaacagg cgcgtcggca gctgttcaga acgcataaag
     8281 taatcttcca ggcaggccgc cagggtatca ccttccagac caactacgcc ctgatagcgt
     8341 tcgccttcgc tcggggtaat ggtgatcacc acgtaaccat tgccgaccag cgttttcagg
     8401 tcggcatttt ctggcatttc gccctgcacg cgcgccacac cgcgcatttg ctggttattg
     8461 ttaccgttaa taaccgccag attcatcgga ccgtcgccct gcagctgtac ggtgatatca
     8521 ccatcaaact tcagcgtagc ggttaacagg ctggtcgcaa ccagcagttc tgccagcacg
     8581 tttttaacgg gctgcggata atcgtggttc tcaaggatct gttgcagggt ttccgaaacg
     8641 gttaccagtt cgccgcgaac ggcaaaattt tcaaacagat agcgatgtaa ttggtcatgt
     8701 tgcggcataa tcatctctct tgcaggtgac agttattcac tgtcgccgtg tttaaatcgt
     8761 aacaggtcgc ggcgctcttt tttgtccggg cgtcggtccg ggtgcggcat ggttaaggca
     8821 ttaagtttac gtgccatcgc cattttttcg cgtttctcta cactttccgc agtctcttca
     8881 tacagcaagg ctgcctcgct ggcggggcga cgctgttcag taatcgcctt tacaatcacc
     8941 gtgcgttcgt catttccctg gcgcagagtg agcgtggcat tcagctcgac gattttgctc
     9001 ggcttgctgc gctgcccgtt gtaatgcacc ttaccgcctt caatcatttt acgggccagc
     9061 gcgcgggttt tataaaaacg ggcagcccat agccatttat ccagtcgaac ctcaacagca
     9121 ggtttctctt tcatggcgtc tccttcacat tagcgagggg atcaggcggc ggtagtcatt
     9181 cagtgacgga tggcgttgat actgtttctc ggcaatcccg gaatcaggat tagtcacgcc
     9241 gaggcagtaa cgaataccaa attgcgcggc agcatcgaga atcgcttcgc tgtcatcaat
     9301 aaacagcgtt ctttcagctt tcagacccgt agcttcggcc accgcatgcc ataaccgctg
     9361 atcctctttc ggataaccaa atgtgtgggt ggaaagtaat aaatcaaggt gtgcgtccag
     9421 acccgtatgc tcaagtttta ccgccaggtt gtgcggatga gcattggtga gcaaaattcg
     9481 ctgcttaccg ctggctttca gtgcctcaag aaacggaatg gtatcttcac gcagtacggc
     9541 gcgcggtccc atctcggtgg tcatcgcaca gatatccaga cccagttgct cactccagta
     9601 atcaagacag taccagttta gcgtatgctg tacgtcgtga tattgctggc gcatatattc
     9661 catcgcttcc tgtggcgtaa ccccgttttt cgcgctccat gtttcaggca ccagcttttg
     9721 ccagaaatag ttatcgaagg cgaggtcgag caacgtgccg tccatatcca gcagaacggt
     9781 atctacgtcc tgccaggcaa tgttgatatg catgagggaa atctccagag tgaagcaatt
     9841 tgcgcgacag ggtagcataa cctgccgcgc aaacgtgtta ttcgataagg ctttctgaag
     9901 gggtgatcag ttgcgggttc aggcagcttt cataataggc ctgaatctcc atcatgcgag
     9961 tgcgatgacg ctggtaacga cgccaggcct gtacgccatt gtaaatggcg ctacccagca
    10021 tcgtcagtag cagcaatgtg gtgctgagat agcgccacag gccggaacga tccgggatcg
    10081 gatgaaggcc aatatgctgc gtaccattgg cgtcagtgaa gatttttgtg acgatacctt
    10141 cggcgttaaa cggcgtatgc atcagcattt gtgccagttt ctggaaagcg ttccactgtt
    10201 cttgcggcgg gtagtcgtaa agtgatgccg aaggccaggg ctgatcaaca aaatcgctgc
    10261 cttcgtcgct gacaatcagg aatccgccgg gggccggact gtttagtgat tgtgccgctc
    10321 gcgccgtttc atgcgtgata aacggcgcgg tggaggttgc caccaggtta tccagcgatt
    10381 ccgcactcac cgggcgtaat aacacattca cgccatcgag cttcccggcg ttggcgcgtt
    10441 ttaccagcgc gtcccagtct ttactgttgc cgagattgac cagcgcattt ttcagtcgca
    10501 cacagtcatc tttggcagaa cataaatccg ctgtcttcag tacaatgtcg ccaaaatcat
    10561 caagcaatac catgccggat ttttgaatcg ctgaacgtaa tgaggcactg acgcgagatt
    10621 catcttccgg tttagggtgc agctggcgat taactgcttc agtcaatgcc gtcgctttgt
    10681 tgaccagttc agattctggt aatggtaatg agcgggcgtc attccagatg atctgcgagc
    10741 agtcaaacgg taaaaaaggt gaattggttt tcgcgctcca ggttccggaa gtgcgaatat
    10801 tacacattcc cgtaccgcta atacgcaatg tatcgcctac ccgcacgcca gcgtcagcca
    10861 gttgttttac gccggtggct tcaatggtct gcgcgccttt catccatgag agagtgaatt
    10921 tcagcggcat atccagcggg atccagaata acagcataaa cagcaccagc agcgagcccg
    10981 cagcgataat cgtactgcgc agccagtgct gtaacggaaa gttttttact tcatcatgca
    11041 gcgaaagata tcgcccctga cgcactacgt ggcggtcgag atagatatca atatcggttt
    11101 gttgaccgag atcctgagca atgtatggct gccagtgtgc gggatagacc aggtcgataa
    11161 taccgagtga aatattgttg atctgttcct gatcgttttc gccaaacaat ccccaacgac
    11221 ggggtgtacc gcgcagacaa tgaatttctc gcagggagga ttttgccggg ggcgcgaata
    11281 gcccccacag acctgcgccc agcagcagta atgcgccgcc tgccagccac ggaacaaata
    11341 catccggggt aatcaggcag aaaaagaaca tcaggaagga ggcgacgatc agcaacgctt
    11401 cacgcagccc gcgcggacga ctcagcgcat attcttcatg cgtttctttg cgaatattga
    11461 gtagctcgat ttgctcactc tcttccccgc gaatcgacgc ctgcgtggac ggaacaggtt
    11521 gcaaggcata gccgcgcgtt tcctgcatgt actcctgcag cgtatgaccg ttgagtgaaa
    11581 taaccaacgg cagcgaatcg gtatgaatca gttcaacggt attctcatcg ttgatgtact
    11641 gttcccagaa ggggggcagg tggacttcta ccgaatcgag gtagtaacgc catttattcg
    11701 gatcgtcggt agagatgccg taacgtgtga tagcgtgtgt cagcatcatg acgttgttgc
    11761 tttcggcatt cagcgccaga gagagcggtg ccgcgctagc tcccgttggg ctaggcacct
    11821 gtaatacctg cgtcaggctc tcaagataat tttcaacggc gctacgttct tcaggtgtga
    11881 gtttacgcgt ttgcgcatcc gcgaaggcgt tggtccaggg cagctgacgc cgtctggatc
    11941 gcacttttat cagccatccc gcaagtagtg agcaggccag caaagcagct aaaaaaatca
    12001 caatggtgct catgctttcc ccatcttact tagtctgtca ggcgtggtca gtcgcaccgg
    12061 acacaataca tgattctgcg gttggctatc ggcaagcgtg gagtttaact tgagcatcat
    12121 taagcacatc acagtaaccg ctgatcatag caaaccctct gaaggcggaa tatcagcaaa
    12181 ttcctggagt tatttcaacc tattgacttt taatttgatt tgaggacgat tatttattaa
    12241 gttacataaa tgtaaataac atgcaattca cattgccgaa agcgtgtccg atatcgcaca
    12301 ataaccgctg atttcacagc atagatccca cgatgagcaa atcattacaa aaacccacca
    12361 ttctgaatgt tgaaactgta gcccgttccc gactgtttac cgtcgagagc gtggatctgg
    12421 agttcagcaa tggcgtgcgg cgtgtttatg aacgaatgcg tccaaccaac cgggaagcag
    12481 tgatgattgt gccgattgtg gacgatcacc tgatcctgat ccgcgaatac gcagtgggaa
    12541 ctgaatccta cgaattaggt ttttcgaaag gattaattga tccgggtgaa agcgtctacg
    12601 aagccgctaa ccgcgagcta aaagaagagg ttggatttgg tgcgaacgat ctgacttttt
    12661 tgaagaagct cagcatggca ccgtcttact tttccagcaa aatgaatatc gtggtcgcgc
    12721 aagatctcta cccggaatcg ctggaaggcg atgagccaga gccgctacca caggtgcgct
    12781 ggccgctggc gcatatgatg gatttgctgg aagaccctga cttctacgaa gcgcgtaatg
    12841 tcagcgcgct gttcctcgtg cgcgaatggt tgaaagggca ggggcgagtg taaatgcgtg
    12901 aagtttgcct gatgcacaac gcttatcagg cttatggggt tcctgcaata tattgatttt
    12961 tcacgatttt gttagccgga taacgcgttc acccgcatcc ggcataaaca aatgcacttt
    13021 gtcagccaac tgaaaaggcg ccgaagcgcc tttttaatca gaacaattcc tgtgcctcac
    13081 cattatcgat aatggtcgtt cccacctcgt gcaccgcctg ttgtgtcggc tgcgtacctt
    13141 cgatgaaata ctcttcgcgg ctgttgccac cattagctaa ctgcccggtg ctgcgatcga
    13201 tattcaccgt cacaataccc ggaggcggcg tcagcggctg ctccggcaca ccttcaagaa
    13261 cggctttcat gtaagcgtcc catgcaggct gggcactctt ggcaccgcct tcgtaacctg
    13321 agatctgatc tttaatcgct ccggaagccg ttgtatgacc gagattacga cggtgatcat
    13381 caaagccaat ccagaccgag gtcacaacgc ccggaccgta acccgagaac cacgcatctt
    13441 tcgaactgtt agtggtaccg gttttcccgc cgatatcgcg acgctgcaaa tcacgacctg
    13501 cacgccagcc ggtaccctgc cagcctggtt caccaaagat attggtgttc aaagcactct
    13561 taatcaggaa tgccagcgga gtgttgatga cgtgcggtgc gtactcctgc gcgccagtct
    13621 tcgccactaa cgcctgattt gcctgcccca gctgcggcat tggtacagaa acattctgct
    13681 gctcgcggga gatagcgaca tcttcaacat cgttattttc cagcacgttc gatttctgcg
    13741 tatcaccgta aatcactgga atatcgcatt ccgggcaggc tactttcggt ttcgcttcga
    13801 aaatcacgcc gccctgatcg ttttcaattt tgctgataaa ccacgggtcc accaggaagc
    13861 cgccgttcgc catgaccgcg tagccgcgcg ccacctgcat tggggtgaag gacgctgaac
    13921 ccagcgccag cgattcggtg tggacaatgt tttgcgccgg gaagccgaag cgttgcagat
    13981 attctgcagc gtagtcgacg cccatcgccc gcattgcacg taccatcacc acgtttttcg
    14041 actgacccag cccctgacgt aagcgaattg gaccagcata ctgcggtggt gagttcttcg
    14101 gctgccagtc agaaccggca cctgcatccc agcgagaaat tggcacatcg ttcaacatac
    14161 ttgccagcgt cagaccttta tccatcgccg cggtgtagag gaacggtttg atgttggaac
    14221 ccacctgacg cagtgcctgg gtggcgcggt taaacttgct ctgattgaaa tcaaagccac
    14281 cgaccagcgc cataacggca ccattttgcg gattgatcga caccagcgcc gagttcactt
    14341 ccggcacttg tgccagccac catgcatcgc caacctgacg aacccagatt tgctgacccg
    14401 tttgcagaac atcggtcact ttacgcggcg tcggtccctg ctgagtatcc gaacggtaag
    14461 gacgcgccca gcgaacgcct tccatactca atgcgacggt cgacccgtcc gccagcatcg
    14521 ccgtcgctcc ctgaggattg gcgctggtga ctgcggcagg cagcagcgga ccataggtcg
    14581 gcagcgcctt cagcgtatcg gtaatcttgt tgttatccca cgccgactcg cccactttcc
    14641 acagcacatt tgccgggccg cgatagccgt ggcgcatgtc gtagtccagc acgttattac
    14701 gtaccgcctg ctgcgcggcc tgctgcactt tgtgggtgat ggtggtgtaa atgcgataac
    14761 cgtcttcata ggcactttcg ccataacggt tatacatctc ctggcgcacc atttcgctca
    14821 ggtacggcgc agagaaagca atctccggcg cgtgataatt agcgttaatc gcctcagtgc
    14881 gtgtctgatc gaactgttgc tgggtgatat acccttcatc cagcatccgc gacagtacga
    14941 cgttacgccg cgcgacggca cgatccattg agtagagcgg attaaaggtg gaaggcgctt
    15001 tcggcaaccc ggctatcacc gccatttcgt tcagcgtcag ttggtcgacc gttttaccga
    15061 aatagacttg tgccgcagca ccgacaccat aggcgcggta accaaggtaa atcttgttca
    15121 gataaagctc gaggatttcg tctttcgtca gcaactgttc aatgcgaatc gcgaggaaga
    15181 cttccttaat cttacgcatc agcgtgcgtt ctggactgag gaagaaattt ctcgccagct
    15241 gctgggtaat ggtactcgcc ccttgtgacg cgtggccgga gaacagcgcc acacttgccg
    15301 cacggaagat ccccaccggg tcaacgccgt gatgctcgta gaagcggctg tcttccgtcg
    15361 cgataaaggc tttcaccatc tccggtggga tttgatccaa cgtaaccgga atacgacgtt
    15421 tctcaccgta ttgagcaatc agctcgccat cggcgctgta aatctgcatc ggaatttgca
    15481 ggcgaacatc ttttaatgtc gccacatccg gcagttgtgg ctcgatgtag cggtataggc
    15541 cataaatcga gcctgctccc agcagaatgc aacagactgc aaggatcaaa aaatacttta
    15601 cgaacttcac tggaaatttc ccatttagtt tcatttgggc agtttataaa caaacgcgcg
    15661 gtagtataaa ggcaagccag gcgcattgat atacccgtca gagttacggg tgataaggag
    15721 atcatcacaa tggcatttaa gatctggcaa attggtttgc atttacaaca gcaagaagcg
    15781 gcagcggttg cgatcgtgcg gggcgcaaaa gaatgctttt tgcaacgctg gtggcggttg
    15841 ccgctggcgc acgacattat caaagatggg cggattgttg atgcgcagca gctggctaaa
    15901 acgttgttac cgtggagtcg cgaactgccg cagcgtcatc acattatgtt ggcgtttccc
    15961 gccagtcgca cattacagcg gtcatttccg cgcccgtcga tgtcccttgg tgagcgggag
    16021 caaacggctt ggctgtcagg gacgatggcc cgcgagctgg atatggatcc ggactccctg
    16081 cgcttcgatt acagcgaaga ctcactcagc cccgcttata acgtgactgc cgcgcaaagc
    16141 aaagagctgg caacgctgct tacgctggca gaaaggttgc gtgttcatgt tagtgcgatc
    16201 accccggatg ccagtgcatt acagcgattc ctgccttttt taccttctca tcagcaatgt
    16261 ctggcctggc gtgataacga acagtggctg tgggcgacgc gctatagctg ggggcgcaaa
    16321 ctggcggtgg ggatgactag cgcgaaggag ctggcggcag cgttatccgt tgatcccgac
    16381 agcgtcgcga tatgtggcga aggcggattt gacccctggg aggccgtttc tgttcgtcag
    16441 ccgccgctac cgccgcctgg tggagacttt gccatcgcgc tggggctggc gcttgggaag
    16501 gcgtactgat gaacccgcca attaattttt tgccctggcg acagcaacgc cggaccgctt
    16561 ttctgcgttt ctggttgctg atgttcgttg cgcctctgct gctggccgtc gggataacgc
    16621 taatactgcg tctgacaggc agcgccgaag cgcgcataga cgcagttttg cttcaggcgg
    16681 aacaacaact cgcccgcagc ttacagataa cgaagccacg tttgctggag caacagcagt
    16741 tacgcgaaca gcgttcgcaa cggcagcgcc agcgacaatt tacccgcgac tggcaatctg
    16801 cgctggaagc actggcggcg cttttacccg agcacgcctg gctgacaacg ataagctggc
    16861 agcagggaac gctggagatc aaggggctga caacaagcat taccgcgtta aacgcactag
    16921 aaacgtcact ccgccaggat gcgtcttttc atctcaatca gcggggagcc acgcagcagg
    16981 atgcgcaggg acgctggcaa tttgagtatc agttaacaag gaaggttagc gatgaacatg
    17041 ttctttgact ggtggttcgc cacatcaccc cgcctccgcc agttttgctg ggcattctgg
    17101 ttgctgatgt tagttacgct catttttcta tcatcgacac accatgaaga gcgcgacgca
    17161 ttaattcgac tacgggcaag tcatcaccag cagtgggccg cactgtatcg cctggtagac
    17221 accactccct tcagcgagga aaaaacgctg cccttttcgc cactggattt tcagttatcc
    17281 ggcgcgcaac tggtttcctg gcatccatcc gcgcagggag gcgagttggc gttgaaaacg
    17341 ctgtgggaag cagtgccgtc ggcatttaca cggctggcag agcgcaacgt cagcgtgagc
    17401 cgtttttcgt taagcgtgga aggtgatgat cttttgttca cgctacaact ggagacgccg
    17461 catgagggtt aaacgctggt tgttggcagg tattgcattg tgccttttaa ccggtatgcg
    17521 tgaccctttt aaaccgccgg aagatctatg ccggattagc gaacttagcc agtggcgcta
    17581 tcaggggatg gtagggcgag gcgagcgcat catcggtgta ataaaagacg ggcaaaagaa
    17641 atggcgacgg gtgcagcaaa actatgtgct ggaaaacggc tggacaattt tacagctgac
    17701 gccagacgta ctaacgctgg gtaccgggac aaactgcgaa ccgccacaat ggttgtggca
    17761 acggcaagga gatacaaatg aagcaatgga tagccgcact actgttgatg ctgatacccg
    17821 gcgtacaggc ggcaaagccg caaaaagtga cgctgatggt ggatgacgtt ccggtagctc
    17881 aggtgttgca ggcgctggct gaacaggaga agttgaacct ggtcgtgtcg ccagacgtca
    17941 gcggtacggt gtcgttacat ctaacagatg ttccctggaa gcaggcacta caaactgtag
    18001 tgaaaagcgc cggactgata acgcggcagg aaggcaacat tctctcagtg cattccattg
    18061 cctggcagaa tgacaatatc gcccgccaag aggcggagca ggcgcgggcg caggcaaatc
    18121 tgccgctgga aaatcgcagt ataaccctgc aatacgccga cgcgggagaa ctggcgaaag
    18181 cgggggagaa gctactgagt gccaaaggga gtatgaccgt cgataaacgc accaatcgcc
    18241 ttttgctacg agataacaaa acggcgttaa gcgcgcttga acagtgggta gcgcaaatgg
    18301 atctgccggt cgggcaggtt gagctgtcgg cgcatattgt caccattaat gaaaaaagtt
    18361 tgcgtgagtt aggcgtgaaa tggacgctgg ccgatgcgca acacgctggt ggcgttgggc
    18421 aagtcaccac gcttggtagc gacctctccg tagcgacggc gacaacgcat gtcggtttta
    18481 acattgggcg catcaacgga cgcttgctgg atcttgagct ttccgcgctc gaacaaaaac
    18541 agcagctgga tattatcgcc agtccgcgtc tgctggcctc acatcttcag cctgccagca
    18601 ttaaacaggg gagcgaaatt ccatatcagg tttccagcgg ggaaagtggc gcgacgtcgg
    18661 tggaatttaa agaggccgtc ctggggatgg aggtcacgcc cacggtgtta caaaaaggtc
    18721 gcatccggct gaaattacac atcagccaga acgttccggg gcaggtgcta cagcaggccg
    18781 atggcgaagt gctggcgatt gataagcagg agatcgaaac gcaggtcgag gtaaaaagcg
    18841 gagaaacgtt ggcgctgggc ggcattttta cccgtaaaaa taaatcgggt cagga
//
LOCUS       DBKEKI010000066        18652 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.066, whole genome shotgun sequence.
ACCESSION   DBKEKI010000066 DBKEKI010000000
VERSION     DBKEKI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.066"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1144)
                     /gene="glmS"
                     /locus_tag="ACUQPX_002382"
     CDS             complement(<1..1144)
                     /gene="glmS"
                     /locus_tag="ACUQPX_002382"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HHV9057077.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSS"
     gene            complement(1306..2676)
                     /gene="glmU"
                     /locus_tag="ACUQPX_002383"
     CDS             complement(1306..2676)
                     /gene="glmU"
                     /locus_tag="ACUQPX_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HHV9057078.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3028..3447)
                     /locus_tag="ACUQPX_002384"
     CDS             complement(3028..3447)
                     /locus_tag="ACUQPX_002384"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HHV9057079.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(3468..4850)
                     /gene="atpD"
                     /locus_tag="ACUQPX_002385"
     CDS             complement(3468..4850)
                     /gene="atpD"
                     /locus_tag="ACUQPX_002385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HHV9057080.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(4877..5740)
                     /gene="atpG"
                     /locus_tag="ACUQPX_002386"
     CDS             complement(4877..5740)
                     /gene="atpG"
                     /locus_tag="ACUQPX_002386"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HHV9057081.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(5791..7332)
                     /gene="atpA"
                     /locus_tag="ACUQPX_002387"
     CDS             complement(5791..7332)
                     /gene="atpA"
                     /locus_tag="ACUQPX_002387"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HHV9057082.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(7345..7878)
                     /gene="atpH"
                     /locus_tag="ACUQPX_002388"
     CDS             complement(7345..7878)
                     /gene="atpH"
                     /locus_tag="ACUQPX_002388"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HHV9057083.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(7893..8363)
                     /gene="atpF"
                     /locus_tag="ACUQPX_002389"
     CDS             complement(7893..8363)
                     /gene="atpF"
                     /locus_tag="ACUQPX_002389"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HHV9057084.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(8425..8664)
                     /gene="atpE"
                     /locus_tag="ACUQPX_002390"
     CDS             complement(8425..8664)
                     /gene="atpE"
                     /locus_tag="ACUQPX_002390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HHV9057085.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(8711..9526)
                     /gene="atpB"
                     /locus_tag="ACUQPX_002391"
     CDS             complement(8711..9526)
                     /gene="atpB"
                     /locus_tag="ACUQPX_002391"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HHV9057086.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(9535..9915)
                     /gene="atpI"
                     /locus_tag="ACUQPX_002392"
     CDS             complement(9535..9915)
                     /gene="atpI"
                     /locus_tag="ACUQPX_002392"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HHV9057087.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(10532..11155)
                     /gene="rsmG"
                     /locus_tag="ACUQPX_002393"
     CDS             complement(10532..11155)
                     /gene="rsmG"
                     /locus_tag="ACUQPX_002393"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HHV9057088.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11219..13108)
                     /gene="mnmG"
                     /locus_tag="ACUQPX_002394"
     CDS             complement(11219..13108)
                     /gene="mnmG"
                     /locus_tag="ACUQPX_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HHV9057089.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(13487..13930)
                     /gene="mioC"
                     /locus_tag="ACUQPX_002395"
     CDS             complement(13487..13930)
                     /gene="mioC"
                     /locus_tag="ACUQPX_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HHV9057090.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14020..14478)
                     /gene="asnC"
                     /locus_tag="ACUQPX_002396"
     CDS             complement(14020..14478)
                     /gene="asnC"
                     /locus_tag="ACUQPX_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HHV9057091.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            14630..15622
                     /gene="asnA"
                     /locus_tag="ACUQPX_002397"
     CDS             14630..15622
                     /gene="asnA"
                     /locus_tag="ACUQPX_002397"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HHV9057092.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(15627..17078)
                     /gene="viaA"
                     /locus_tag="ACUQPX_002398"
     CDS             complement(15627..17078)
                     /gene="viaA"
                     /locus_tag="ACUQPX_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HHV9057093.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSQEAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17072..18568)
                     /gene="ravA"
                     /locus_tag="ACUQPX_002399"
     CDS             complement(17072..18568)
                     /gene="ravA"
                     /locus_tag="ACUQPX_002399"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HHV9057094.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELQEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPADWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4459 a         4923 c         4675 g         4595 t
ORIGIN      
        1 gagaagaacc cggaacgtta cagattgcca gcgaaccaag gtaacccagc tctttcgaca
       61 gacgcaggcc cgccagggta tccgcggttt cgccagactg tgacaaggtg atcatcaggc
      121 tgttacgacg cacggcagat ttacgatagc ggaattcaga ggcgatttcg acgtcgcacg
      181 gaatacctgc cagcgattca aaccagtagc gggaaaccat accggagtta taagaagtac
      241 cacaggcgag gatctgaata tgctcaacct tcgacagcag ttcgtcggcg ttcggtccca
      301 gctcgcttaa atcaacctga ccgtggctga tgcgcccggt aagggtgttt tttatcgcgt
      361 tcggctgttc gtagatctct ttctgcatgt agtgacggta aataccttta tcgcccgcat
      421 catattgcag attggattcg atatcctgac gttttacttc cgcgccagtt ttatcgaaga
      481 tgtttaccga acggcgagtg atttccgcaa tatcgccctc ttcaaggaag ataaagcgac
      541 gggtcaccgg caacagcgcc agctggtcag aagcgataaa gttttcgccc atacccaggc
      601 caatcaccag cggactacca gaacgtgccg ccagcagggt atccgggtga cgggagtcca
      661 tgatcactgt accgtacgca ccgcgcagct gcgggatagc acgcagaaca gcctcacgca
      721 gagtcccgcc ttgtttcagc tcccagttca ccagatgggc aatcacttcg gtgtcggttt
      781 cagaaacgaa ggtatagcca cgcgctttta actcttcacg cagcggttca tggttttcga
      841 tgatgccatt atgcaccacc acaatgtgtt cagaaacatg cggatgcgcg ttagcttctg
      901 aaggttcacc gtgggtcgcc cagcgagtgt gagcaatacc ggtgccgcca tgcagaggat
      961 gttcttccgc tgcctgagcc agcatctgga ctttaccgag gcgacgcagg cgagtcatat
     1021 gaccttccgc atcaacaacg gccagaccgg cagagtcata tccgcggtat tccagacgac
     1081 gtaaaccttc aagaaggatt tctgctacat cacgttgcgc gatcgcgcca acaattccac
     1141 acatagtttt tgattccgat ttatatcgtt gtcggtcaac ctgtatgccc gttttttcgg
     1201 gcgccccgag ccttgtagag agtggggtta tttttatagt tactgcttgt gggagggaga
     1261 ttatgttatc tcctcatccc agatgaccgg attacccggc cagaattact ttttctttac
     1321 cggacgacgc cagccttctt tctgagtctg cggcacacgg ctgatagcta atgcattttc
     1381 gccgacatta cgcgtcacag ttgtacccgc agcaatggtc gcgcctttgc ctactgttac
     1441 cggggccacc agctgagtgt cggaaccgac aaacacatcg tcgccgataa tggtcttaaa
     1501 tttattcgca ccatcgtagt tgcaggtaat ggttcccgcg ccgatgttaa cgttatcgcc
     1561 aatttccgca tcgcccaggt aagtcagatg cccggctttc gtgcctttac ccagacgcgc
     1621 ttttttcatc tcaacgaagt taccaacgtg tgcaccttcc agcaactcag caccaggacg
     1681 cagacgggca aacgggccaa tagtacaggc cgctgccaga ttcgcgtctt ccacaacggt
     1741 atacggactg atttcgcaat catcgccaat cacgctgttt ttaatcacgc aaccggtgcc
     1801 gattttcacg cgatgaccga gagtcacgtt gccctcgatg ataacgttag tatcaatttc
     1861 aacatcgcgc ccgtgagtaa gcgtaccgcg cagatcaaaa cgcgccggat cgcgcagcat
     1921 aacgcctgct aacagcagtt tttcagcctg ttcggactgg taaacgcgct ccagacggga
     1981 gagttgcagg cggttattca caccttctac ttcgcttaaa cgttgcggat gaacggcgac
     2041 gatttcacgc ccttcctgat acgccagcgc aataatgtcg gtgatgtagt attcgccctg
     2101 agcattattg ttggtcagct tcgccagcca gcgtttcata tctgcgccgt tggcaatcag
     2161 aatgccggtg ttgatctcct gaatctgacg ctgctcgtcg gtggcatctt tgtgctcaac
     2221 aatgccggta actttgccat tttcacgggt gatacgtcca taaccggtcg gatcatccag
     2281 tttcaccgtc agcagaccaa tgccaccctg gggtttagca tcacgcaggc gctggagtgt
     2341 ttcgacagag atcagcggca cgtcgccgta gagcattaaa atgtcttcat catcggcaaa
     2401 gaaaggtgcg gcctgctgca ttgcatgacc cgtacccagc tgctctgcct gaagcaccca
     2461 gttaaggttg tcgtctttca gcgcctgttt aagcagatcg ccgccgtgac cgtacaccag
     2521 gtgaacgtgc gctgcgccta attcattcgc agcatcaatg acatgctgaa ccatcgcttt
     2581 cccggcaagg gtatgcagca ctttcggaag atcggaatac atgcgcgtgc ctttgcctgc
     2641 ggcaaggatc actacgctca tagcattatt caacatacgc gtcctgactg taatttgaga
     2701 acgaatttaa accgcttcac cttgaaaaaa ctacattttt ttcatcgtga aatggacaga
     2761 ggataaattg ttcaataacg gattatcccc gctgacgaaa gtgccatttt cgaccatcgt
     2821 cacgcctttt gtctgcagaa aattaaggca gtaccttcat ttaagcgtca ggtggatgtt
     2881 tttgctctta tttcgatcaa tgaataaacg ggaaaacagg ggtgttttat atcagcagga
     2941 tctatgtgaa cgctcttcag gacggttcaa cgcgtaaaaa aaagccagcc tgtttccaga
     3001 ctggcttttg tgcttttcaa gccggtgtta catcgctttt ttggtcaact cgataacgcg
     3061 cagctgcgcg atcgctttgg ccagttccgc agacgcctga gcgtaatcta cgtcgccgtg
     3121 agagctgcta atgtgctctt cagccttacg tttcgcttcc atggctcgcg cttcgtcgag
     3181 atcctggccg cgaattgcgg tgtcggccag aacggtcacg ttgccaggct gcacttcaag
     3241 aatgccgcca gacagataga taaactcttc gtgaccgtgc tgtttcacga tgcgaatcat
     3301 accaggctta atggcggtga gcagcggtgc gtggccaggg tagatcccca gttcaccttc
     3361 gctacccgtt acctggattt tctcgaccag accagagaac atttgttgct ctgcgctgac
     3421 gacgtccagg tggtaagtca ttgccatatc accctccgat taaggcgtta aagttttttg
     3481 gctttttcca cagcttcttc gatggaaccg accatgtaga acgcctgctc cggcaggtga
     3541 tcgtattcgc cttccatgat gcctttaaag ccacggatgg tgtctttcag ggagacgtat
     3601 ttacccggag aaccggtgaa tacttctgcc acgaagaacg gctgggacag gaagcgctgg
     3661 atcttacgag cacgcgctac caccagtttg tcttcttcag acagttcatc catacccagg
     3721 atggcgatga tgtctttcag ttcctgataa cgttgcagga tggactgaac gccacgcgcg
     3781 gtgtcgtagt gttcctgacc aaccaccagc gggtccagct gacggctggt ggagtccagc
     3841 gggtcaacgg ccgggtagat acccagagac gcgatctgac ggctcagtac cacggttgcg
     3901 tcaaggtgcg caaaggtggt tgccggagac gggtcagtca agtcatccgc aggtacgtat
     3961 accgcctgta cggaggtgat agaaccagtt ttggtggagg tgatacgttc ctgcagaacg
     4021 cccatctctt ccgccagggt cggctgataa cctaccgctg aaggcatacg gcccagcagt
     4081 gcggatactt ccgtaccggc cagggtgtaa cgatagatgt tgtcaacgaa cagcagaacg
     4141 tcacgacctt cgtcacggaa tttctcagcc atggtcagac cggtcagagc aacgcgcaga
     4201 cggtttcccg gcggctcgtt catctggcca tacaccaggg atactttgtc gataacgttg
     4261 gagtcggtca tttcgtggta gaagtcgtta ccctcacgag tacgttcacc tacgcccgca
     4321 aacacagagt aaccggagtg ctcgatcgcg atgttacgaa tgagctccat catgtttacg
     4381 gttttaccta cacccgcacc accgaacaga ccaactttac cgcccttagc gaacggacac
     4441 atcaggtcga taactttgat accggtttcc agcagttcct gagagtttga cagctcttcg
     4501 taggaaggtg ctgcgcggtg aatcgcccaa cgctcttctt caccgatctc gcctttcatg
     4561 tcgaccggtt cacccagtac gttcatgata cggcccagag tcgctttacc taccgggact
     4621 tcgatcgggt gttcgaggtc ttttacatcc agaccgcgac gcagaccgtc ggaggaaccc
     4681 attgcgatgg tacgcacgat accgccgccg agctgctgct gaacttccag caccagacgc
     4741 tcattaccat tttgcacctc aagagcatcg tacacgcgcg gtacggcatc ctgagggaat
     4801 tcgacgtcaa ctacggcgcc gattacctgg acaatctttc cagtagccat cttaaatcct
     4861 ctacgaaata acctgtttaa accgcggcgg cccccgagac gatctcggtg agttcctgag
     4921 taatgctggc ctgacgagct ttgttgtata ccaactgcag ctctttaatc aggctgccgc
     4981 cattgtcggt cgcggctttc atcgccacca tacgggcggc ctgctcgctg gccaggtttt
     5041 caaccacgcc ctgataaacc tgagattcga cataacgacg cagcagggta tccagcaacg
     5101 ccttcggatc gggttcgtac aggtaatccc aggatttatg tttcagatca tcatcatctg
     5161 atgccggtaa cggcagcagc tggctgatgg tcggaacctg agacatggtg ttaataaatt
     5221 tgttgctgac aatgtaaagc ttgtccagac ggccttcgtc gtaggcctgc aacatcactt
     5281 ttaccggacc gatcagttcg gacagggaag ggttatcccc catgccggtg acctgggcaa
     5341 caacattgcc gcccacggag ttgaagaacg acacgccttt cgagccgatc attgcgaggt
     5401 cgcattgaac gcctttgtcg gtccaggtct tcatttccgc cagcagtttt ttgaacaggt
     5461 taatgttcaa accaccgcac aaaccacggt cggtcgacac caccaggtag cccacgcgtt
     5521 taacgtcgcg gtcttccagg taagggtgct tatattccag attaccgtgt gcaaggtgac
     5581 caatcacttt gcgcatggtt tctgcataag gacggctggc cgccatgcga tcctgcgatt
     5641 tacgcatttt ggaagcggcg accatctcca tcgctttagt gatcttttgc gtgttctgga
     5701 cgcttgcgat cttactacgt atctcttttg cgccggccat gagcttctcc tcaatgcctt
     5761 gcggcctgcc ctaaggcaag ccgccagacg ttaccaggat tgggttgctt tgaaggaatc
     5821 gaggatgcct ttcagcttgc cttcgatttc gtcgttgtag ccaccggtct ggttgatctc
     5881 ttgcatcaac ggagcgtgat cacggtcgac gtaagccagc agagcggctt cgaagctgcc
     5941 aattttcgac agttcaacat ccgccaggta accacgttct gctgcgaaca gaaccagaga
     6001 ctgctgcgca acggacatcg gcgcatactg tttctgtttc agcagttcgg tcactttctg
     6061 accgtggtca agctgcttac gtgttgcatc gtcaaggtcg gatgcaaact gagagaacgc
     6121 tgccagttca cgatactgtg ccagagcggt acggatacca ccggacagtt ttttcatgat
     6181 cttggtctgt gctgcaccac caacacggga tacggaaata cccgggttaa ccgcaggacg
     6241 aataccggcg ttgaacaggt tggtttccag gaagatctga ccatcggtaa tggagattac
     6301 gttggtcgga acgaacgcag aaacgtcacc cgcctgagtt tcgataatcg gcagtgcggt
     6361 cagagaaccg gttttccctt tcacttcacc tttggtgaag gcttcaacgt attcggcgtt
     6421 aacacgtgca gcacgctcca gcagacgaga gtggaggtag aaaacgtcgc ccgggaatgc
     6481 ttcacgtcct ggcggacgac ggagcagcag ggagatctga cggtaagcaa cagcctgttt
     6541 agacaggtca tcgtaaatga tcagcgcatc ttcaccgcgg tcacggaagt attcgcccat
     6601 tgcgcaaccg gcatacggtg ccaggtattg cagtgcagcg gattcagacg cggttgctac
     6661 cacaacgatg gtgttagcca gtgcgccgtg ctcttccagt ttacgtacca cgttagaaat
     6721 ggtggacgct ttctggccga tagcgacata gatacatttg ataccggaat cgcgctggtt
     6781 gatgatggca tcgatagcca gtgcggtttt acctgtctga cggtcaccga tgatcaattc
     6841 acgctgacca cgaccgattg ggatcatgga gtcaacggct ttataaccgg tctgtaccgg
     6901 ctgatctacg gactgacgtt cgataacgcc cggagcgatt gcttctacag cagagaagcc
     6961 gtcgtgatcc agcggacctt taccgtcgat tggtgcaccc agagtgttaa ccacacggcc
     7021 cagcaggcca cggccaaccg gaacttccag gatacggcca gtacacttaa ctttcatgcc
     7081 ttcagcaagg tcagcgtacg gacccataac aaccgcacct acagagtcgc gctcgaggtt
     7141 cagtgcgata gcgtaacggt tacccggcag ggagatcatt tcaccctgca tacaatcggc
     7201 caggccgtga atgcggataa caccgtcact tacagaaaca atagtacctt cgttgtgagc
     7261 ttcactcaca acattgaact gagcaatgcg ctgcttgatc agttcgctga tttcggtgga
     7321 attcagttgc atgctccagt ccccttaaga ctgcaagacg tctgcaaggc gctcaagacg
     7381 accgcgtacg ctgccatcaa tgaccatatc acccgctcgg atgataacgc ctgccattac
     7441 agacttatcg attttgcaat tcagcttaac tttgcgtgac agacgttttt ccatcgcagc
     7501 agaaattttc gcgagctgtt gttcactcag tgcggcagcg gaaatgacgt ctacctcagc
     7561 ggtagcctca ctcacggcac gaaggtgaat aaactgctcc agaacatccg ggagcgcgtt
     7621 aagacgacca ttttcagcca ttacccgaat caggttctga ccgttttcgt ccagttgctc
     7681 accacaaact gcgataaacg actcggcgag cgtttctggc gcaagcgcgc cagagagaag
     7741 ctctgccatt tgttcgtttt tggttacctc ggcggcaaac gccagcatgt cctgccagcg
     7801 ttctacactt tggtgttcga cggcaaagtc aaaagctgct ttggcgtagg ggcgagctac
     7861 cgtaataaat tcagacatca gcccctccct ccttacagtt cagcgacaag tttatccacg
     7921 atgtcgctgt tagcagcttc atccacggaa cgttcgatga tcttctcggc gccagcaaca
     7981 gccaggatag caacttgctt acgcagctct tcacgggcac gtttacgctc ggcttcaatt
     8041 tctgcctgcg cctgggccac gattttagta cgttcctgtt ctgcctcagc tttcgcttcg
     8101 tccagaatct gcgagcggcg tttgttcgcc tgctcgatga ttacctgggc ttccgctttc
     8161 gcttttttca gctggtcggt cgcgctggcc tttgcaaggt caaggtcctt atgtgctcgt
     8221 tctgcggaag caaggccgtc agcaatttct ttttgacgtt tttcgatggc tgccattaat
     8281 ggcggccata cgtacttcat gcagaacaga acgaacagga caaacgcgat ggcctggccg
     8341 aggattgttg cgttaagatt cacagcacaa tgcctctatt tagttaacgt tctgatattg
     8401 ctctttaaat aaaagcaacg cttactacgc gacagcgaac atcacgtaca gacccagacc
     8461 tacagcgatc atcgggatag catccaccag acccataacg ataaagaact gagtacgcag
     8521 cagaggaatc agatcaggtt gacgcgctgc gccttccaag aatttacccc cgaggatgcc
     8581 gataccgatc gcagcaccga ttgccgccag acccatcatc acagcggcag ccatgtacag
     8641 cagatccata ttcaggtttt ccatgacagt ctccagtttg tttcagttaa aacgtagtag
     8701 tgttggtaaa ttaatgttct tcagacgcca tcgacagata gacgatcgtc agaaccatga
     8761 agatgaaggc ttgcagcgta atgatcagga tgtggaaaat ggcccacggc acattcagga
     8821 tccactgtga ccaccacggc aacagaccag caatcagaat gaaaatcagc tcaccggcat
     8881 acatgttacc gaacagtcgc aaaccgagtg aaactggttt ggacagcagg cttacccctt
     8941 caaggattaa gttgacagga atgaacgccc agtgattgaa cggctgcagc gtcaactctt
     9001 tcgtgaagcc gccgatgcct ttcattttga tgttgtagaa cagaatcagg ataaatacgc
     9061 ccagtgccat agacagcgtt acgttcacgt ccgcagacgg aaccacacgc agtgcaggca
     9121 gacccagtac atgttcagca atgtacggca gcaggtcgat aggcagtaaa tccatcaggt
     9181 tcatcaggaa tacccagacg aagatcgtca gggccagcgg agcaatcagc ttgcttttgc
     9241 catggtacat gtctttcacg ctaccattaa caaagccgat caccagctca atcgcggtct
     9301 gaaacttacc tggcacaccg ctggtcgcct ttttggctac gctacggaat aaaaccagga
     9361 acaacagacc cagcaccacc gagaagaaca tggagtcaat attgattgtc cagaaggtgg
     9421 ctggggggtt ttgtggatcc accagcgaga atgtacgcag gtccagctga aggttattca
     9481 ggtggtgtcc tatgtaatcc tgcggcgtca tattttctga agccatgatg ccttttaccc
     9541 tttgttgtta attacagccg gtgccagtat ctgaaccacc agcaccaaaa cccacgtaac
     9601 gatcagcggc aagaataccg cctttaaaac cgccaacgcc accaccagta acaccaacat
     9661 cgccagaact ttgaaagctt cgccaaatgc gaatgtccaa gccacccggc ctttcgctgg
     9721 tgtatgcgcc tggtgacgcc aggcaaatat cataaacaaa acgttaggca gaaagactgc
     9781 caggcccccg cttattgcag agacgcccca gaaggggtct ttgaggctga acagcaatcc
     9841 acttgctatc accaccagta actgaacgag cagaagcttc cgagcaacgt ttcgactcac
     9901 gagcgacaca gacatcacgt ttttcactcc tgctcccttc gaggtatgcc gcgtgtcgta
     9961 taaaactttc tttaaggctt agagtcaagc atcaaaaagc ggtcaaatta tacggtgcgc
    10021 ccccgtgatt tcaaacaata agtagccaaa aggtgaataa atgtttaaat atttttccag
    10081 tgcatacaat tgcgactttt ccgctaaccc tgttcgatca tgaaaaactg taaataacgc
    10141 gtaaacactg gtgataaagc gtgcttcaga tcacatattg cgcatgttcg cgcacagcat
    10201 atttatttac ttggcaaatg atgcctttgc aattttatga tatttcagtc taaaaacaga
    10261 tactgtttta ataaatgata tttacacaac aaaaaccacc cattgacatt tttaataatg
    10321 ttttaacaac caatgatggt tcttagcgcc gatttttagc agactgatat tttcactaat
    10381 gacttatttt ctgcttacca aaaaaagcca cgttatcttg ttgatgcaaa agagtgaacg
    10441 tggcgttaaa tgtaaccagt tatatcagta gaaaacctgg ttgttgttaa cagtctaacc
    10501 ggtcaatttt ttatgatttt tttgataaaa attaaatttt atttgcttta atcaccacca
    10561 gatgacgttc gccatccagg gctggaacct gaagtttaac cactgattcg acctgatatt
    10621 cttcgggcaa caaagcgatt tcatcttccg gcatttgccc tttcagcgcg tagaaacggc
    10681 cttgctcacc aggaagatgg tggcaccagc tcaccatatc gttcagagag gcaaaagcgc
    10741 ggctaattac gccatcaaat ggcggctctg aaggaaactc ttctaccctg ctctgtactg
    10801 gttcaatatt ctccagttta agctcatgtt gcacctgacg aaggaaacgc acgcgtttac
    10861 caaggctatc caacagagtg aaatgggctt caggacgcac gatagagagt ggaatgcctg
    10921 gcagtcctgg tccggtgccg acatcgataa accgttcacc ttgcagatac ggtgccacca
    10981 caatgctatc gagaatatgg cgtaccagca tctcattagg atcgcggacc gaagtcaggt
    11041 tgtacgcttt gttccattta tgcagcatat tcacgtaggc aataagctgg tttttctggt
    11101 gatcagtaag cgaaatacct gcgtctttca gcagtaagga gagtttgttg agcacggtga
    11161 ttacctgttc ttgatgcgtt gcctggtaag cgggtgctta ccaggcattt ttaatgcgtt
    11221 atgcgctacg acgcagcata ccctgttttt tcagccacac cagcagaatg gagatggccg
    11281 caggcgtgac gccagaaata cgcgaagcct ggccgataga ggccggtttg tgatcgttaa
    11341 gtttggcgat cacttcgtta gaaagaccgg atacctggcg gtaatccagt gtcgcgggta
    11401 gcagggtgtt ctcgttacgc agctgctttt cgatctcatc ttgctggcgc gcgatataac
    11461 cttcgtattt aacctgaatc tcaacctgtt ccgccgcctg ttcgtctgtc aacgcagggg
    11521 caaacggcgt cagcgtggtt aatttttcat aagtcatttc cggacgacgc agcagatctt
    11581 caccactggc ttcacgggaa agcggcgcag tcaggtgagc attcacttcg gctgcagctt
    11641 ccgccgacgg ggttacccag gtcgatttca gacgctgacg ctcacgctcg atattctcaa
    11701 gtttctcgtt aaagcgcgcc caacgttcgt catccaccag gcccagttca cgaccgattt
    11761 cagtcaaacg cagatccgca ttatcttcgc gcagcatcag acgatattct gcgcgcgaag
    11821 taaacatacg atacggttct ttggttccta aagtgcacag gtcatcaacc agtacgccga
    11881 gatacgcctg agaacgtgcc ggagcccaac cttctttgtc agcagacaga cgggcagcgt
    11941 taagaccggc cagcaaacct tgcgcagcgg cttcttcgta accggtagtg ccgttaatct
    12001 gaccagcaaa gaacagcccc tggataaact tgctctccag cgtcggtttc aggtcgcgag
    12061 gatcgaagaa gtcatactca atggcataac ccggacgcac gatcttcgcg ttttccatcc
    12121 cctgcataga gcggacgatt tgcatctgca catcgaacgg caggctggtg gagataccgt
    12181 tcggataaat ttcattagag gtcagtcctt ccggttcaag gaagatctga tgctgatttc
    12241 tgtctgcgaa gcgcatgact ttgtcttcga tcgacgggca gtagcgtggg ccgacacctt
    12301 cgatcacccc tgcatacatt gggctacgat cgaggttact gcggatcaca tcatgggttt
    12361 tctcgttggt atgagtgata taacacggca cctgctgggg atgctgggac gcattgccca
    12421 taaacgagaa taccggcatt gggttatcgc catgctgttg cgccagcacg ctaaagtcga
    12481 tggttcgagc atcaatacgc ggcggagtac cggttttcag acgaccaacg cgcagcggca
    12541 gttcacgcaa acggcgagag agcggaatgg atggcggatc gccagcacgg ccaccgctgt
    12601 aattatccag accgatatga attttgccgt cgaggaacgt cccaacggtg agcaccacag
    12661 ctttggcacg gaacttcagt cccatttggg taacagcacc gaccacgcga tcgttttcga
    12721 caataagatc ttcaaccgcc tgctggaaga tcatcaggtt cggttggttc tccagcgccg
    12781 tacgtaccgc ctgacggtag cgcacacgat ccgcctgagc tcgggtagcg cgaaccgccg
    12841 gtcctttgct tgcgtttagt atcctaaact ggatacccgc ttgatcgatc gctttcgcca
    12901 tcagaccgcc gagtgcatcc acttctttta ccagatgtcc cttcccaata ccgccgatcg
    12961 ccgggttgca gctcatctgc cccagagtgt cgatattgtg tgtcaaaagc agagtctgtt
    13021 gacccatacg cgccgcagcc atcgcggcct cggtgcctgc atgacccccg ccaatgatga
    13081 tgacgtcaaa aggatccgga taaaacatgg tgattgcctc gcataacgcg gtatgaaaat
    13141 ggattgaagc ccgggccgtg gattctactc aactttgtcg gcttgagaaa gacctgggat
    13201 cctgggtatt aaaaagaaga tctatttatt tagagatctg ttctattgtg atctcttatt
    13261 aggatcgcac tgccctgtgg ataacaagga tccgactttt aagatcaaca acctggaaag
    13321 gatcattaac tgtgaatgat cggtgatcct ggaccgtata agctgggatc agaatgaggg
    13381 gttatacaca actcaaaaac tgaacagcgg ttgttctttg gataactacc ggttgatcca
    13441 agcttcctga cagagttatc cacagtagat cgcacgatct gtatacttat ttgagtaaat
    13501 taatccacga tcccagccat tcttctgccg gatcttccgg aatgtcgtga tcaagaatgt
    13561 tgatcttcag tgtttcgcct gtctgttttg caccggaatt tttgagttct gcctcgagtt
    13621 tatcgatagc cccacaaaag gtgtcatatt cacgactgcc aataccgatt gcgccaaagc
    13681 ggaccgcaga aagatcgggc ttctgttcct gcaatgcttc atagaaagga gaaaggttgt
    13741 ccggaatatc tccggcaccg tgggtggagc tgataaccag ccagatcccc gaggcagata
    13801 aatcttccaa cagcggaccg tgcagcgttt cggtggtaaa acccgcctct tccagctttt
    13861 cagccaggtg ttctgctaca tattcggcac cgccgagggt gctgccgctg ataagagtga
    13921 tatctgccat aaaccgccac ctttattaag agtggcgtat tgtacgctgt gaacgcgttg
    13981 ggatctacct gtggaaaagt atgggattaa aaaagctgat cagggcttga tggtacgcat
    14041 gatcgggttt tgcaggacga tcaatgtctc ggtggactga atttcatcaa ttgtttggat
    14101 cttgttgata agtacatgct ggagagcgtc gatcgaacgg cacataactt ttataaagat
    14161 gctgtagtgg ccggttgtgt aataggcttc agtgacttca tcaaggcttt ccagctttgc
    14221 cagcgcggaa gggtagtctt tggcgctctt taatataatg ccgataaagc agcctacgtc
    14281 ataaccgagc tgcttcgggc tgacatcaat acgcgccccg gtaatgatcc ccgcctgctt
    14341 cattttctct actcgaacgt gaatcgtccc cggactgacg ccaaattgtt tcgccagttc
    14401 ggcgtaagcg gtgcgcgcat tgcccattaa tgcttccagg atgccacggt ccagattgtc
    14461 gatcagataa ttttccatag gattttctta tgcagattga tgattcattc tattttagcc
    14521 ttctttctta atgaatcaaa agtgtgttag gctttttatt gaatgattat tgcatgtgtg
    14581 tcggtttttg ttgcttaatc ataagcaaca ggacgcagga gtataaaaaa tgaaaaccgc
    14641 ttacattgcc aaacaacgcc aaattagctt cgtgaaatct cacttttctc gccaactgga
    14701 agaacgtctg gggctgatcg aagtccaggc gccgattctt agccgtgtgg gggatggcac
    14761 gcaggataac ttgtcgggct gtgaaaaagc ggtgcaggta aaagtgaaag ctctgcctga
    14821 tgcccagttc gaagtggttc attcactggc gaagtggaaa cgtcagacct tagggcaaca
    14881 cgacttcagc gcgggcgaag ggctgtacac gcacatgaaa gcccttcgcc ccgatgaaga
    14941 ccgtctttct ccgttgcact cggtctatgt tgaccagtgg gactgggaac gcgtaatggg
    15001 cgacggtgag cgtcaattct cgactctgaa aagcacggta gaggcgatct gggcgggaat
    15061 taaagcaacc gaagctgcgg ttaacgaaga gtttggcctg gcaccgttcc tgccggatca
    15121 gattcacttc gtacacagcc aggagttact gtctcgttat ccggatctcg atgccaaagg
    15181 gcgtgagcgg gcgattgcga aagatcttgg cgcggtattc ctcgtcggga ttggcggcaa
    15241 gttgagtgat ggtcatcgtc acgacgtgcg cgcaccggat tatgatgact ggagcacccc
    15301 gtcagagctg ggccatgcgg gcctgaacgg cgatattctg gtgtggaacc cggtactgga
    15361 agatgcgttt gagctttctt ccatggggat ccgcgtggat gccgacacgc tgaagcatca
    15421 gctggcgctg accggtgatg aagatcgtct gcagctggaa tggcatcagg cgctgctgcg
    15481 cggtgaaatg ccgcagacca tcggcggcgg tatcggccag tctcgtttga ctatgctgct
    15541 gctgcaactg ccgcatatcg gccaggttca gtgtggagta tggccagctg ctgttcgcga
    15601 gagcgtccct tctctgctgt aataatttat cgccgccagc gtctgagcag gcggcttcgc
    15661 atcccggtat caaagcgcca gatatgatcg aaaatgcgca tgatgccggg tttgccgtgt
    15721 gccgacatcg ccacggcatg aaagcgatgc tgatgtaccc gctgcagctc tttcacttta
    15781 ctcgtcacgt cgtcaggcaa ccgctgagcg ataaaatcag aaatcaccac cgcatcggca
    15841 tcaaaccatt ccctgctttg caagcgttcc ataatggcgc gaaaacaact ggcaagatcg
    15901 gtgccgccac gaaactgctg gcttaaaaaa cggattgctt gttcgatgcc ttgtgggcct
    15961 gaaagctcat aacggacgat ctcggtggaa aatagcataa tataacagcg ccggttttca
    16021 gcgagagcaa tgcgcatcaa agccaggcag aacgctttcg cacactgttc attaaagccg
    16081 cccattgagc cggaagtatc cacacagaca ataaacggcc cgcgcggctg ttcatcgtaa
    16141 tctttatgta ccaccggacg ttcgagcact ttttcacgcc acgactcacc gtgcaggcga
    16201 taggtgagca actgtttttc caccagccga cggtaaaact catactccag ttccgttatc
    16261 cctagtgtcg ccagttctgg cggcaggaga cgtaaaatat catcgctttg ttgcagacca
    16321 tcaacctgct caggaaccgt cgccggttcg cgcaccatgg tgcggaaggt ttccatctgc
    16381 gcatcgttgc gcggtattga tttggcttcc tgagaacgcc ccagctgctc tgccaggcgt
    16441 ttcagttccg gctgttcgtt aagaaattca ccgtatttca caatcaactg atagtcgcca
    16501 cgtttaagct gaccggcgct catatcccac agacgaccag ctgcggtatt gttatctgcg
    16561 agaatcggtt caagttgtcc gctcagcgtc atgcgttcct gaacttcact taacagttgt
    16621 tcgcgttctt cttctaatag ctgttgattt aacgtcgttg cttgcacgat cagacttaaa
    16681 cgccaacgct ggagaaaaag cgtgtgtaac gctgaagtta ttgtgctgtt agcatcaacc
    16741 aactgccggg cttgttctgc ccatggggaa ttcagacgat gcagtaagtc caggatctgt
    16801 ggtagctgca caataaactg cggcgtggag aggagctggc tttgctgata gcacatcacc
    16861 tcttcggtga gttccggcgg gactcgggca tctttcagcc gactgcgcag cgcctcacgc
    16921 cagcggggaa catcatcagt gattgccgcc ttcagtcgtg ggaatttttc aaagaagacc
    16981 gccagctgcg gtgaggccag cagtgcgatg atcatctctt cgatcaatcc ctcttcgctg
    17041 acggccagca tcacattaag cgtatccagc gttagcattg ttgtgcctgg cgaatctgtg
    17101 caccgacatc ctgtaggctg gcttcaatgc gacctaacca gtcggcaggg ataaacagac
    17161 atttttgctg ttcgctgaat aaagcgtgtt gcttacgcca gtcgctttca agctcttcca
    17221 gttgttgttt tatttcacct ggcagacctt cagccgatga accgggaagt gccagcgtac
    17281 tgccttgtaa acttacatcg cgtacaacaa gatgttgggt gctatcaact tccagattca
    17341 gtttctgggc aaagccgata ccgttcagtt tgccgcgaat ttcaccacct ttactcagcc
    17401 actgttccag cgcgctacgc tcaaaggaga tatgaaccac ttccatatca tgcagtttta
    17461 acggtttttg cagcagcaga gtcagagtgg aagcagtaac gttaacagga agttgatact
    17521 gctgacgacg gctgaaaatg ccgcccagac gaattaacgt taaggctgtt ttatcgcttt
    17581 gttgctgctg gagttgcagg tgacgttgca caatcgcgcc caggcgggtc aacatccctt
    17641 gctgttgcca ggcatgaccg gtcatcaata catcaatttg ttgttgtatc aaattcaggc
    17701 tttgcgcgtc ataccacagg caatctttca gcaaaatgag atcaaccggg gcaacagcac
    17761 tgcgaccgct aaaaaaggcg ctggcctgca ataagcggat cgcttttttc cagcgacgat
    17821 ccgagacata aggcgcatcc ggtaatttat ccagttgctg gcgcagcata aaaatcagct
    17881 caaatacatg atcgggcagc gtaatttcac caatctcttt ctgccagcgt tcatattctt
    17941 catctgtgac ctgcaaggca tcaggaaccg gattgtcgtt ttcatcctgt tgactggtca
    18001 gcatggagcg gaaattcgct ttatcttgca ctttatctaa ccatagacga atcagcatgc
    18061 ggtcatataa cgcttccaga ctgctgtctg cttcctgcag ctcgttggag gccgccacca
    18121 gtagacgcat cgggattttt tctacgtgtg caccgttgcg gaactggcgc tcgttaatgg
    18181 cggtgagcaa ggtattaaga attgccgggc ccgctttcca gatctcatcc agaaagacga
    18241 tttctgcttc cggcaggtaa ccgctggtta aacgttcata gcgcccttca tcttttagcg
    18301 cctgaataga aaggggacca aaaacttctt ccggcgtgga gaagcgggtc atcagatatt
    18361 caaacgcgcg ggcattctga aaggcgaatt ttaagcgccg ggcgatcaaa cttttggcaa
    18421 tacctggcgg gccaaggagg aacacacttt caccacttaa tgccgctaat aaacacaagc
    18481 ggatggcgtg gctacgttca taaagcccct tttccaacga actgctcagg cgggaaattc
    18541 tttccgctaa taaatgaggg tgagccataa tgaagtggcg tcctttcgtc aaaagttctg
    18601 cgtaaattgc gagtatagac gtttcttgct ggtggctaaa atagtctcaa ag
//
LOCUS       DBKEKI010000067        18225 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.067, whole genome shotgun sequence.
ACCESSION   DBKEKI010000067 DBKEKI010000000
VERSION     DBKEKI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.067"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            502..1158
                     /gene="pphA"
                     /locus_tag="ACUQPX_002400"
     CDS             502..1158
                     /gene="pphA"
                     /locus_tag="ACUQPX_002400"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HHV9057095.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1159..1350)
                     /locus_tag="ACUQPX_002401"
     CDS             complement(1159..1350)
                     /locus_tag="ACUQPX_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HHV9057096.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1506..1691)
                     /locus_tag="ACUQPX_002402"
                     /pseudo
     CDS             complement(1506..1691)
                     /locus_tag="ACUQPX_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
     gene            complement(1809..3248)
                     /gene="rsmF"
                     /locus_tag="ACUQPX_002403"
     CDS             complement(1809..3248)
                     /gene="rsmF"
                     /locus_tag="ACUQPX_002403"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HHV9057097.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(3328..5961)
                     /gene="yebT"
                     /locus_tag="ACUQPX_002404"
     CDS             complement(3328..5961)
                     /gene="yebT"
                     /locus_tag="ACUQPX_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HHV9057098.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5930..7213)
                     /gene="yebS"
                     /locus_tag="ACUQPX_002405"
                     /pseudo
     CDS             complement(5930..7213)
                     /gene="yebS"
                     /locus_tag="ACUQPX_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            7343..7840
                     /gene="msrC"
                     /locus_tag="ACUQPX_002406"
     CDS             7343..7840
                     /gene="msrC"
                     /locus_tag="ACUQPX_002406"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HHV9057099.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFVRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7937..8635
                     /gene="proQ"
                     /locus_tag="ACUQPX_002407"
     CDS             7937..8635
                     /gene="proQ"
                     /locus_tag="ACUQPX_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HHV9057100.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8655..10703
                     /gene="prc"
                     /locus_tag="ACUQPX_002408"
     CDS             8655..10703
                     /gene="prc"
                     /locus_tag="ACUQPX_002408"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HHV9057101.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQTAPVK
                     "
     gene            10895..11776
                     /gene="htpX"
                     /locus_tag="ACUQPX_002409"
     CDS             10895..11776
                     /gene="htpX"
                     /locus_tag="ACUQPX_002409"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HHV9057102.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11822..13194)
                     /locus_tag="ACUQPX_002410"
                     /pseudo
     CDS             complement(11822..13194)
                     /locus_tag="ACUQPX_002410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13371..14162
                     /gene="kdgR"
                     /locus_tag="ACUQPX_002411"
     CDS             13371..14162
                     /gene="kdgR"
                     /locus_tag="ACUQPX_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HHV9057103.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(14305..14544)
                     /locus_tag="ACUQPX_002412"
     CDS             complement(14305..14544)
                     /locus_tag="ACUQPX_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HHV9057104.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            14703..14846
                     /gene="mgrB"
                     /locus_tag="ACUQPX_002413"
     CDS             14703..14846
                     /gene="mgrB"
                     /locus_tag="ACUQPX_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HHV9057105.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14921..15208
                     /locus_tag="ACUQPX_002414"
     CDS             14921..15208
                     /locus_tag="ACUQPX_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HHV9057106.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            15878..16021
                     /locus_tag="ACUQPX_002415"
     CDS             15878..16021
                     /locus_tag="ACUQPX_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HHV9057107.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            16034..16243
                     /gene="cspE"
                     /locus_tag="ACUQPX_002416"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             16034..16243
                     /gene="cspE"
                     /locus_tag="ACUQPX_002416"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HHV9057108.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            16409..17218
                     /gene="rlmA"
                     /locus_tag="ACUQPX_002417"
     CDS             16409..17218
                     /gene="rlmA"
                     /locus_tag="ACUQPX_002417"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HHV9057109.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(17215..17781)
                     /gene="mntP"
                     /locus_tag="ACUQPX_002418"
     CDS             complement(17215..17781)
                     /gene="mntP"
                     /locus_tag="ACUQPX_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HHV9057110.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(17778..17900)
                     /locus_tag="ACUQPX_002419"
     CDS             complement(17778..17900)
                     /locus_tag="ACUQPX_002419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057111.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
BASE COUNT         4829 a         4353 c         4584 g         4459 t
ORIGIN      
        1 ctattgccag tacacctcaa ccccaaatcg taaaggaaac cgtagcacgt cgtatgcaag
       61 aacgtgccac ggctggctga tggatgttcg atagcgcgag tttgaatgaa aatcagccgg
      121 agatgatttt acataattgc tacggaatta ttcaatacag gaattgcttg cgtctgcatg
      181 gattgacctg aaatattccc gaaaatttct ctaaaaaact cgaaaaaaat ggtaacttgt
      241 tgaatgtatt aatatgcaat ggtacgtgtc agggattaaa agatgaacgt aaatttattc
      301 aacgcattaa ttttaaaggg ttttattgtt tgttgacgaa aacaggaatc gtgttcggtc
      361 tctttttatc tgttaaaagc cagaagcatt tccttcgctg actttatagt caaccataac
      421 acacactcta ctgtctgagt ccagcgtttt ttaacattct tgttaagatt atgtgatctt
      481 tagcgcggga ggaaaatatt gatgaaacag cctgcgcccg tttatcagag aattgcgggt
      541 catcaatggc gacatatctg gctttctggc gatatacacg gttgtcttga gcagttgcgc
      601 cgcaaattat ggcattgtcg ttttgatccg tggcgagatt tacttatctc agtgggagac
      661 gttatcgatc gtgggccgca aagtttacgt tgtttgcagt tactggaaca acattgggtt
      721 cgtgcggtaa gaggcaatca tgaacagatg gcgatggatg cgctggcatt ccagcagatg
      781 tctttgtggt tgatgaatgg cggcgactgg tttattgcgc tggcagataa tcaacagaaa
      841 caagcgaaaa cggcgctgga aaaatgtcag catttgccct ttattcttga agtacacagt
      901 cgcaccggca agcatgttat tgctcatgcc gattatccag atgatgttta tgaatggcaa
      961 aaggacgttg atttgcatca ggtcttgtgg agccgctcgc gattaggtga acgcccaaaa
     1021 gggcagggaa ttacaggtgc tgatcatttc tggtttggtc atacaccgtt gcgacatcgc
     1081 gtggatattg gcaacctgca ttatattgat accggtgctg tctttggggg cgaattgact
     1141 cttgtgcaac tgcaataatt aaaaatcacc atactcctgt gctggtcgcc agaaaccatc
     1201 tataaaatcc tcaatcggaa aacaaccgcc atggcggatc cgttgatcgc tcatagaata
     1261 aagacactgc tgttccgtgt tgtagacatc cacaacaata tcttcacaac cgccatccag
     1321 gtagcaaaca aaaagtacca gcgcgaacat ttcatccccg aagtgtggtg ccgtaccgtt
     1381 aagtttagga gagattttac aacgggggaa taaccaggac aaataacccg ccatgccgac
     1441 gggttatttt tggatcaggc aagacgcata acccaggcat cagattgttg atcgtaacgg
     1501 gtacgctaaa ggacagagtg ttcgccattt agcagggcag ggatactggt ttcagcatct
     1561 caggcatcca gttgcataca taaatcgggg tcagatttac aaggaatggt taacgttcga
     1621 tcacccggcg attcaccgtg taattcgccg tcgtcgattt caaaagcgcc tatgcgcaca
     1681 ctggttttca tcttcgtgct ctccatttag ctgccgattg tggtacgacc agtccgatcg
     1741 cacaattttt cagcctagtt gaggccgccg aatgtgccag tcaaaaagtg cgcttttttt
     1801 gtgcgctgtc atgcgttact ggtaaaaagt ttcccgtccc gcaccagttc acgcgggtag
     1861 ctgtttttca gtcgcgaacc aatccgtttg gctaaaccaa tcggctgatg ctggaaagta
     1921 accaacacgt catccgccac tggcgcggct tgcgggtaaa catcgcgccc gcgataccac
     1981 tcctccgctt cctgcggtgt cagttcaaaa gcgtttacat tgtcggggga ggcatgggca
     2041 ataaccgctt catgctgcca gcgataacct ttgttatgcg tttcggcaag tttaatcccc
     2101 aagcgagaaa atcggacttt accgatcagg gcttcaatgc ctaccgggaa caaccacagt
     2161 tctttgtcgc gttgccagag tcgcaggttt ccatcccagt ttaagccaac acttgcagcc
     2221 gcctgacgaa tttgtccggc ttcgcgatct ttcaccgggc tgaacgggaa attgcccact
     2281 ttgtattttg gggcgggtaa ggcggggatc gcctgagttt tacgcagacg agcaacgaag
     2341 aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa aaccttcttc ggtcagcgcc
     2401 ttgtttgcac cagggaagag atcgccaagt ggtaaaaact ctacttcgtc ggggtaagtc
     2461 tctttcagcc accggcaaac ggcttcgttt tcttcccggt ttaaggtaca ggtcgagtaa
     2521 accagcgtac cgccaggacg taatgcatga aaggcgctgt caatcagttc ccgttgggta
     2581 gctgcgattt cctgattgct ttcgggtgac cagtttttta gcgcatcggg atctttacgc
     2641 accacgcctt cgccggagca gggagcgtcc agcaaaatgg catcaaacat ttctggcact
     2701 gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa cattactgat gccacagcgg
     2761 ctgatattgg catgtaacac ttttacccga ctggcggaaa actcattggc aaggattgcc
     2821 ccttcgttat tcatccgcgc ggcaatttgc gtcgtttttg agcctggcgc agcagcgaca
     2881 tccatcaccc gctgtggtgc attaccgtca gcaaacaagg cggcgacggg caacattgaa
     2941 ctggcttcct gaatataaaa caggccactt aaatgctcgg tggtactacc caatggcaat
     3001 gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac accacggaat tggcgtaagc
     3061 gtccagccat aaggagcggt taattgcagg aaatcagcaa cggagatttt cagcgtatta
     3121 acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc aaatgagagc
     3181 gtcgaaggca tagcttcgcg catttgtgtc agaaacgcgt ccgggaaata aacggtgtgt
     3241 tgggccacga gcatgtacca ctggcaaaaa aacaggcgcg cagtttagca taaacgctcc
     3301 ggcgcaggca cgccggagca gtgggtatta tttgggaagc gcagttcccc attcacgcca
     3361 ctctttcggt tcactttcct gcaacaggaa gtgtttgcct tcctgggctt tcggtgcaag
     3421 tggcgtaccc ggaggcgtgg cgaaggcgat gccgccacgg ataaattggt taaaggtgcc
     3481 ggttttcact acgccgcccg tcagaccaaa gtccagactg taacccgatg ccaaccagaa
     3541 gacggaattg ttacgcacca ggtgttgata gcgtttactg atgcgcatcg caatcatcac
     3601 gcgatctgac aatgtcccca gcgtcattcc ggtaaccgta cctacttcca gaccacggaa
     3661 cagcacaggc gtaccgatgc ctaacgaacc ggcttccggc gcttcaacaa taatgcttaa
     3721 accatctagg taacgcgaat cagtaatggt ggcctcttgt aattcaaagt cgcgacgagg
     3781 attgccacgg cctggttcga cgttgatata cggctggagg atagtatcta gatgctcaac
     3841 gccagctgcc gagatttgcg gtgtgaccac tgagaagcgc gtaccaccgc gagcaaaggt
     3901 ctggacatat tccggataga gcaccgcctt tgcctgtact tcattgcgtg cggtaatcag
     3961 atccagcgtc tggatttgcc cgatatcaat accgagatag cgaattggca tcccgaccgc
     4021 cagttttccg gcatcgaaag cgtgaagcgt aatctgcccg ccaaccgcac gggccgctgt
     4081 ttcggaagca tacagaattc gcttgtcgcc tttacgctga ctggcgctgg caccactgag
     4141 gttatcgaag ctaatggccc cctttaatgc tctggagagc ggggatgcct gtacggtcag
     4201 gccactacca ttcagctgaa ctttcgcccc gccttctgcc cagaacacgc tgttgctggt
     4261 caaaaggttg cgatattccg gtttaatgtg cagatcgata tcgaacgcgt tagctcgcgg
     4321 acgcacggta ataacttcac caacttcaaa tttacggtac agcactaccg atcctgcctg
     4381 cacatccggc agcgtctctg cactcaaact cacggttgtg gtgggtaaat cgctaaggct
     4441 gttctccagc gctttttcca gattggcata cagtggatag ctggctttca tctcgccttt
     4501 atcgcccggc agaatacgta tcccgccgtt aatccattct gaggcgctgg caccgagaaa
     4561 ctcaacgcca tccagcccca ccttcacgtc gacacggctg ttgacgacaa atttgctatc
     4621 gccttttacc agttctcgat gctgaggctc gatggcgacg gtaaaggtga cgcctttgct
     4681 ggtgagttta cgatcgataa cctggcctac ctgcacgccg tgaagaatga gcggctgacc
     4741 cgcatcaata ccgtaacttt ccggtgctgt cagggttagt gtcagaacgt ccggctcttg
     4801 cagcagggct ttttcgcccg gcacaacaac gaactctttg cgtggctcgc catcgccggg
     4861 caccaactca aaggtttttc cggtcagcag ggcgctgaga ttagcatcgc taagggataa
     4921 tttcgggttg cgtaattcga tgcgggtatt ttcacgaagc agggtaacga cgctgggatc
     4981 aacggtcatt tccccagtga ctttaccacc aggatttaaa tccagtttag tcagctgtcc
     5041 gacttccagc ccctgataca ttaacggcgt cgagtcggcg gttaatcctg ctccactcgg
     5101 cagttccagt tttattatta ctccacgctg gctgtgggcc agatcttcat acagaccaaa
     5161 ggtatcttcc gcctcggcag gtttcgactc ttcaggtgaa tcgaaggcaa tcgcaccgtt
     5221 aaccagtgcc gccagacttt ccagtttcac cttcgcgcca ctgatactga cgttggcatc
     5281 aacgccggaa acgttccaga aacggctacc ttttttcacc agatcggtaa aacgccgctc
     5341 gatcaggaca tcaatcacca cgccttgctt gttgggattg atggcatagt cgtagacttt
     5401 tcccaccggg atcttgcgga aatagaccaa tgaaccgctg ttcagcgaac cgagatcggg
     5461 ggcttgcagg tggatcatca gatcgccatt gtccagccga tatttcggtt gggtatcgag
     5521 cgcgacaaag tgatcctgct ctttaccttt acccggcatc atgccgatat agttcccacc
     5581 gacgagggcg tccaacccgg atacacctgc caacgatgct tttggcgtca ccagccagaa
     5641 ctgagtctct tcgcgcagcg catctttcat atcggacttg atgctgacct tgacttcaat
     5701 cttacgaaga tcgtcgctga ggctgatatc ctgcactgtt ccgacttcaa cgccctgata
     5761 acgaacaggc gtacggcccg gaacaatacc atccgccgac ataaagtcga tggtgacggt
     5821 attaccccgg tcctgataac tgtcccaaat cagccaaccg gcaatcatta gcgcgatgaa
     5881 aggcagcagc cagaaaggtg agatacggcg tttattttta atctgcgctt cagtcgtcga
     5941 agcgggcgtt tcctgactca tgtgcatccc aaagtaagcg gctgtccagc cattccacag
     6001 caagaatagt caaaattacg gcagcgccaa aataaaactc agccggtccc atagtaaaag
     6061 cgaggatctg atcgcgatta atcagcgaca tggttaaaga tatgacaaac aggtcgagca
     6121 tcgaccagcg tccaatctag gtcaccatcc gcagtaacag aatgcgtgtg cgtaaacctt
     6181 gctggcattt aaaatgaatg ctcaacagta aagtgaacat gacgatcact ttagtaaacg
     6241 gtaccagaat actggcgata aacacgattc ccgcaaccgc aatgttgcta cttgccagcg
     6301 acataattcc ggaaagaatc gtatcttcct gccggccacc attcagataa atgatagaaa
     6361 taggcaatag gttggctggt aacaacaaaa cgattgacgc taacagcgcc gcccagcatt
     6421 tttgcaaact atgacgacga cgcaggcgta gcgggatatg gcaacgcggg cagagaccac
     6481 gctgatcagg atagccggta aaatggcacc caagacagac acgaagtttc tcgtccctac
     6541 gcgtagcggg gcgctgcgga taatatcgct cccacagctc ctcaacatta agatgtgaca
     6601 acgtcaccgt cgttaaaatc accaacgcca caaaagagaa caagccgaca cccgcctgga
     6661 tatgggcata atcctgtacc tttatagaag caacgccaat gccgaccagg tagatatcca
     6721 gcattaccca ctctttaagt cgctcaagca tcagcagtac cggacgtaaa ttcattccca
     6781 gtcggttacc aaaccataaa tatgctatgg aggacaccag aatgaggggg gcaccaataa
     6841 cgcagaaaaa gaccattgat cccgttatcg catcgccctg tttggtcatt tgccagatgc
     6901 cttgcatgac gttggcgtca atacgaatgc ctaacagcca gatatgcaac agcggttcgc
     6961 cccaggcaaa cggcataagc aacagcatgg tgaaggccat tgccgccagg cgcgttagcg
     7021 accagtcgcg cccgtcgcga atttttgcct gacagcgcgg acaataggca ctttgatgag
     7081 aatttatctc gggcaggcta aacagcatgt cacattgcgg gcaacgttgg taatcaccac
     7141 gcggcagttc ctcgccgatt gccctcactg ttatcttttt tgtcggcgta atttgtggtg
     7201 tgttaagagc catgacgtgc caaatgtaat aagaattaac attaatctta acccatgatg
     7261 ggaatgatct tgagcattaa cgcatttaat gcttatttta ataggctggc agttccaggt
     7321 aagacaacca agtgattaaa taatgaacaa aacagaattt tacgcggatt taaatcgcga
     7381 ctttaacgcg ctgatggcgg gagaaaccag ttttctggca acgcttgcga acaccagtgc
     7441 gttgttatac gagcgtctca ctgacgttaa ctgggcaggt ttttatttgc ttgaggacga
     7501 tacactggta ctcggaccat ttcagggcaa aattgcctgt gtccggatac ccgtcgggcg
     7561 cggggtgtgc ggcacagcgg ttgcccgcaa tcaagtgcag cgtatcgagg atgttcacgc
     7621 gtttgacggg catattgcct gtgatgcggc gagtaattct gaaattgttc tgccgctggt
     7681 ggtgaaaaat cagattattg gtgttctcga catcgatagt accgtcttcg ttcgctttac
     7741 agacgaggac gagcaaggct tacgtcagct tgtggcacag cttgaaaaag tgcttgcaac
     7801 gacggattac aaaaaattct ttgcgagcgt cgcaggataa tcaacggata acgtagcatt
     7861 tactggtagc gtcattataa tgacgcctgt tcatgcctgc gcttgttggc tacgtccgtt
     7921 gtaatcagga aatttcatgg aaaatcaacc taagttgaat agcagtaaag aagtaatcgc
     7981 gtttctggcc gaacgttttc cccactgttt cagtgcggaa ggtgaagcgc gtccgctgaa
     8041 aattggtatt tttcaggatt tggtcgatcg tgttgccggg gaaatgaacc tgagcaaaac
     8101 gcaattacga tccgctttac gtctctacac ttcgagctgg cgttatcttt acggtgttaa
     8161 acccggcgca acgcgtgtcg atcttgacgg caacccatgc ggtgagctgg acgagcaaca
     8221 tgtagagcat gctcgcaagc agcttgaaga agcgaaagcg cgtgttcagg cacagcgtgc
     8281 tgaacagcaa gcgaaaaaac gcgaagctgc cgcagctgct ggtgagaaag aagacgcacc
     8341 gcgccgcgaa cgcaagccac gtccgaccac gccacgccgc aaagaaggcg ctgaacgtaa
     8401 acctcgtgcg caaaagccgg tagagaaagc gccaaaaaca gtaaaagcac ctcgcgaaga
     8461 acagcacacc ccggtttctg acatttcagc tctgactgtc ggacaagccc tgaaggtgaa
     8521 agcgggtcaa aacgcgatgg atgccaccgt attagaaatc accaaagacg gcgtccgcgt
     8581 ccagctgaat tcgggtatgt ctttgattgt gcgcgcagaa cacctggtgt tctgaaacgg
     8641 aggccgggcc aggcatgaac atgtttttta ggcttaccgc gttagctggc ctgcttgcaa
     8701 tagcaggcca gaccttcgct gtagaagata tcacgcgtgc tgatcaaatt ccggtattaa
     8761 aggaagagac gcagcatgcg acggtaagtg agcgcgtaac gtcgcgcttc acccgttctc
     8821 attatcgcca gttcgacctc gatcaggcat tttcggccaa aatctttgac cgctacctga
     8881 atctgctcga ttacagccac aatgtgctgc tggcaagcga tgttgaacag ttcgcgaaaa
     8941 agaaaaccga gttaggcgat gaactgcgtt caggcaaact cgacgttttc tacgatctct
     9001 acaatctggc gcaaaagcgc cgttttgaac gttaccagta cgctttgtcg gtactggaaa
     9061 agccgatgga tttcaccggc aacgacactt ataaccttga ccgcagcaaa gcgccctggc
     9121 cgaaaaacga ggctgagttg aacgcgcagt gggacagtaa agtcaaattc gacgagttaa
     9181 gcctgaagct gacaggaaaa acggataaag aaattcgtga aaccctgaca cgccgctaca
     9241 aatttgccat tcgtcgtctg gcgcaaacca acagcgaaga tgttttctcg ctggcaatga
     9301 cggcgtttgc gcgtgaaatc gatccgcata ccaactatct ttccccgcgt aataccgaac
     9361 agttcaacac tgaaatgagt ttgtcactgg aaggtattgg cgcagtgctg caaatggatg
     9421 atgactacac cgttatcaat tcgatggtgg caggtggtcc ggcagcgaag agtaaagcta
     9481 tcagcgttgg tgacaaaatt gtcggtgttg gtcaaacagg caagccgatg gttgacgtga
     9541 ttggctggcg tcttgatgat gtggttgcct taattaaagg gccgaagggc agtaaagttc
     9601 gtctggaaat tttacctgct ggtaaaggga ccaagacccg tactgtaacg ttgacccgtg
     9661 aacgtattcg tctcgaagac cgcgcggtta aaatgtcggt gaagaccgtc ggtaaagaga
     9721 aagtcggcgt gctggatatt ccgggcttct atgtgggttt gacagacgat gtcaaagttc
     9781 aactgcagaa actggaaaaa cagaatgtca gcagtgtgat catcgacctg cgtagcaatg
     9841 gcggtggggc gttgaccgaa gcggtatcgc tctcaggtct gtttattcct gcgggtccca
     9901 ttgttcaggt ccgcgataac aacggcaagg ttcgtgaaga cagcgatacc gacgggcagg
     9961 tgttctataa aggcccgctg gtggtactgg ttgaccgttt cagtgcttcg gcttcagaaa
    10021 tctttgccgc ggcaatgcag gattacggtc gtgcgctggt tgtgggtgaa ccgacgtttg
    10081 gtaaaggcac cgttcagcaa taccgttcat tgaaccgtat ttacgatcag atgttacgtc
    10141 ctgaatggcc agcgctgggt tctgtgcagt acacgatcca gaaattctat cgcgttaacg
    10201 gcggcagtac gcaacgtaaa ggcgtaacac cagacatcat catgccgacg ggtaatgaag
    10261 aaacggaaac gggtgagaaa ttcgaagata acgcgctgcc gtgggatagc atcgatgccg
    10321 cgacttatgt gaaatcagga gatttaactg cctttgaacc ggagctgctg aaggaacata
    10381 atgcgcgtat cgcgaaagat cctgagttcc agaacatcat gaaggatatc gcgcgcttca
    10441 acgctatgaa ggacaagcgc aatatcgttt ctctgaacta cgctgtgcgt gagaaagaga
    10501 ataatgaaga tgatgcgacg cgtctggcgc gtttgaacga acgctttaaa cgcgaaggta
    10561 aaccggagtt gaagaagctg gatgatctac cgaaagatta ccaggagccg gatccttatc
    10621 tggatgagac ggtgaatatc gcactcgatc tggcgaagct tgaaaaagcc agacccgcgg
    10681 aacaaaccgc tcccgtcaag taatatcaat caggcacaag aaattgtgcc tgatttttta
    10741 acagcggcaa gatgccgtaa atcagatgct acaaaatgta aagttgtgtc tttctggtga
    10801 gttacgcact atccagactt gaaaatagtc gcgtaaccca tacgatgtgg gtatcgcata
    10861 ttgcgttttg ttaaactgag gtaaaaagaa aattatgatg cgaatcgcgc tcttcctgct
    10921 aacgaacctg gccgtaatgg tcgttttcgg gctggtactg agcctgacag ggatacagtc
    10981 gagcagcgtt caggggctga tgatcatggc cttgctgttc ggttttggtg gttccttcgt
    11041 ttcgcttctg atgtccaaat ggatggcatt acgatctgtt ggcggggaag tgatcgagca
    11101 accgcgtaac gaaagggaac gttggctggt caatactgta gcaacccagg ctcgtcaggc
    11161 ggggatcgct atgccgcaag tggctatcta ccatgcgccg gacatcaacg cttttgcaac
    11221 cggtgcgcgc cgtgatgcct ctctggttgc tgtcagcacc ggtttgctgc agaacatgag
    11281 cccggatgaa gccgaggcgg taattgctca cgaaatcagc cacatcgcca atggtgatat
    11341 ggtcaccatg acgctgattc agggcgtggt gaacaccttc gttatcttta tttcccgtat
    11401 tctggcgcag cttgccgcgg gttttatggg cggaaatcgt gatgaaggtg aagagagcaa
    11461 cggcaacccg ctgatctact ttgcggttgc aacggttctg gaactggtgt ttggtattct
    11521 ggcgagcatt atcaccatgt ggttctcgcg tcatcgtgaa ttccatgctg atgccggttc
    11581 ggcaaaactg gttggtcgcg agaaaatgat tgccgcgctg cagcgcctga aaaccagcta
    11641 tgaaccgcaa gaagcaacca gcatgatggc tttctgcatt aacggtaagt cgaaatcgct
    11701 cagtgagttg ttcatgactc acccgccgct ggataaacgt attgaagctc tgcgtacggg
    11761 tgaatacctg aagtaatatg aagtaaatac taaaaagcgc gtcctgatcg acgcgctttt
    11821 tttatgccct ggctcgtggc tgagtgatac gtaaaccact gacacaggca gcaatcactg
    11881 ccagaatagc cgcagccatc agcgagacgt gcgtaccatt atcaccaaac tgatttagca
    11941 tcagcgccac cagcgccgcg ccactactct gacccagtag acgagccgtt cctaacatgc
    12001 cactggcacc accgctacgt tcgcgcggag cggaggtaat aatggtgtgg ttattgggtg
    12061 actggaataa gccaaatcca gcgccacata agatcatcgg ccagataata ttgatatccg
    12121 caggtgacgc gggcagcaga accagggaaa aaagccccgc agccatgatg aacaacccta
    12181 aagcacccag caatcctgca tgtacgcgtt caatcaaata gcctgccagc ggagccatca
    12241 ccatcgttgc taacggccac ggtgtcagaa gtaaacctgt ttcgacttca ctacgcccga
    12301 gcacggtttg caggtaaaag ggcagggaaa ccattgccag catttgtgcg cagaaagagc
    12361 aaacagatgt gcaaatagaa agtgaaaaca gcgggatacg cagtaaatcc accggtagca
    12421 gcggtacggg aagagaaagc tggcggcgaa taaagaaaat accaacaaca accattacca
    12481 ccagttccgc agcaattaac gtcagcgatt gcccctgagc gaaaccactc aacgcagtga
    12541 taagcaggcc gaaggttaac gcgttcatca cggcgctggg caggtcgaaa cggggtttac
    12601 tggcgcgaga accattgggt ggcagaaaac gcatcgccag aagcagggcg ataataccta
    12661 acggtacgtt gattaaaaat aaccatttcc aggatgcgat ggagaggatt gctgcagcaa
    12721 ttgtcggccc ggcagcagaa gagacggcaa caataaacga gtttatgccc atccctctcc
    12781 ccagaaaacg ttgtggatag atcaggcgga taagtgcggt attaacgctc atcaacgctg
    12841 caccgccgaa accttgtatg acacgcgcaa gggtgagcat ttgcagcgaa tcagaaaggg
    12901 agcagaacag tgaagacaac agaaaaacga ccagaccgca ttataaatac gtcgatagcc
    12961 aaacatatcg cccagaaacg aaaacgagag cagggagatg acaatggcga tttgataggc
    13021 gttcactacc cagatggaac tggctggtgt ggcatgaagg tccgtggcga ttgttggcag
    13081 ggcgacgttg gcgattgcgc cgtcaaggac agccatcgaa ataccaatca caatggttaa
    13141 tatcgcaccg tatcgctggg gcaatggcag gccgtcggcc tgaacttttg gcataggaat
    13201 tttatatctt tggtgaataa tcaacagggg tatgattcta gcactgatat tcacggtgga
    13261 tgtcgcagaa ttgtaactaa ccggcaaaag atggattgcg accaccctgg tgcgaaatta
    13321 taataaaaaa cagttctgat ttttataaaa cactcgcaat gaggtgataa atggctaacg
    13381 cagatctgga taaacagcct gattctgtat cttccgtgct aaaagttttt ggcattttgc
    13441 aggcgctggg tgaagagcgc gaaataggga taaccgagct gtcgcagcgc gtcatgatgt
    13501 caaaaagcac cgtttatcgc tttttacaga ccatgaaaac cttaggttat gtggcgcagg
    13561 aaggggagtc ggagaaatat tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt
    13621 tacaaaacgt cgatttaatt cgtagcgcag atatccagat gcgtgagatc tcccgcctga
    13681 ccaaagaaac tatccacctc ggcgcactgg acgaagacag tattgtttac attcacaaaa
    13741 ttgactctat gtacaatttg cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca
    13801 gcaccgcgat tggtaaggta ctgctggcat ggcgcgatcg cgatgaagtg aagcaaattc
    13861 ttgagggcgt ggagtataaa cgcagtaccg agcggaccat caccagtaca gaagcgttat
    13921 tacccgttct ggaccaggtg cgcgagcagg ggtatggcga agataacgaa gagcaggaag
    13981 aagggctgcg atgcattgcg gtaccggtat ttgatcgctt tggcgtggtc attgccggtt
    14041 tgagcatctc cttcccgacg ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa
    14101 tgttgcatac cgcagcgcgc aaaatttctg cccaaatggg ttatcacgac tatccgttct
    14161 gatgagagta agaacctgtc ggaatatcaa acagacaggt tctttattta gcatgagaaa
    14221 aataaagttg aaggtgcgtt atattaaacg cgcttgctat aagaatattt tactcatgag
    14281 tgagaatctg gttatttatt acccttaacc attatcgacc acgatattgc ttttacgtaa
    14341 cagtgggcaa tctgttatcc ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct
    14401 ggtgcctctg gcagtcagat aggtacattg caaacctaat cctgcggcat tctctttgct
    14461 tccaatcaaa acgccatatc cgctgagtaa taatcctatc cataccagtg ctatcagcat
    14521 aactgtgcga atgatgaatc gcattacaac ctcttctctt tttatgttcg cttaatcgta
    14581 gcggcaatat gcgctgaagc aagctactca ttccgaaaaa gcacgaatat cgacatagtt
    14641 aggcgctgtt taactaacgc atgctagttt aatgacataa ggtaggtgaa gcggagattg
    14701 gagtgaaaaa gtttcgatgg gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt
    14761 gggcgcaggt attcaacatg atgtgcgatc aggatgtaca atttttcagc ggaatttgtg
    14821 ccattaacca gtttatcccg tggtgatatc attttttcag cactagattt tcttcctctc
    14881 cagcagtggt agaatagcgc ctctactcag aggtggtgaa atgaacgaag ttgtaaattc
    14941 aggcgtgatg aacattgcgt ctttggttgt atcggtggtg gttcttctta tcgggctcat
    15001 cttgtggttt tttatcaatc gtgccagttc acggactaac gaacagattg aactgcttga
    15061 ggcgttgctg gatcagcaaa aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa
    15121 cgaaccagag aaagcagata aaaagaccat tgagagtcaa aaatcggttg atgacgaaga
    15181 tattattcgc ctggtcgccg aacgataatc cttcctcaat cttgagtaac cccgtcgttg
    15241 aagggttact caattcatcc tgattatacg cactgtcatg gtgcaactgc ttcaaattaa
    15301 aactctggtc tgacttcaaa taattatcat attatttatt tgcgtaacgg gatatcagaa
    15361 aaagatattt tcctcattaa tttattagga tgtttacatc agatttgtga ttaagcgtgg
    15421 tattatttat tagcgaaacg tttctctctt gatttttttg ctcactcatc aatttttctt
    15481 attttaaatt tacaatccct ttggggattg acttctcttt agggtaattg atagccgtta
    15541 actgactgtt ttatgagaaa aagtgatata actttttatt cattgcatag cagaaaatgt
    15601 gatattgcac gcactatgta ataacttctc ccactggcct ggaacaactg aacttattga
    15661 actatgttag aaactacgcc agtttaagta tctgcctgaa ctggcaaggt taagcacaat
    15721 gatatatcgg cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga
    15781 tttgtgcgtc tgatcgagac atgtttaaaa atggcttgcc ataattaacg ttgtatgtga
    15841 taaaagattt cgggttaaac gaggtacagt tctgtttatg tgtggcattt tcagtaaaga
    15901 agtcctgagt aaacacgttg acgttgaata ccgcttctct gccgagcctt atattggtgc
    15961 ctcatgcagt aatgtgtcag ttttatctat gttatgcctg cgggcgaaga aaacaatcta
    16021 aggaattttt caaatggcaa agattaaagg tcaggttaag tggttcaacg agtctaaagg
    16081 ttttggcttc attactccgg ctgatggcag caaagatgtg ttcgtacact tctccgctat
    16141 ccagggtaat ggcttcaaaa ctctggctga aggtcagaac gttgagttcg aaattcagga
    16201 cggccagaaa ggtccggcag ctgttaacgt aacagctatc tgatcgaatc cactgatctg
    16261 aagtgtgaat acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc tttttactat
    16321 gctttatctt cgctcctggc gttcggatat ttgcccgccg cgtgattcgc gttacacttg
    16381 cggcctttag tatcctgccg gagttgtcat gtcgttttcc tgtccacttt gccatcagcc
    16441 tctttcgcgt gaaaaaaaca gctatatctg tccccaacgg catcagtttg atatggcgaa
    16501 agaagggtat gtcaatctgc tgcccgttca gcataaacgg tctcgtgatc cgggcgacag
    16561 cgcggaaatg atgcaagcac gccgcgcatt cttagatgcc ggacattatc agccgctgcg
    16621 tgatgcaatt gtcgcccaac tgcgggaaag gcttgatgaa aaggccacgg cggtgctgga
    16681 tattggctgt ggtgaagggt attacacaca cgcatttgcc gatgcgttgc ccgaaatcac
    16741 cacgtttggc ctggatgtct cgaaggtagc gataaaagcg gcggcgaaac gctatccgca
    16801 ggtcactctt tgtgtcgctt ccagccaccg tttgccgttt tccgatacca gtatggacgc
    16861 cataatacgt atttacgcgc cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg
    16921 cggctgggtc attactgcca cgccgggacc gcgacatttg atggagctga aggggctgat
    16981 ttacaatgaa gtacatcttc atgcacctca tgcagaacaa ctggaaggtt ttacattaca
    17041 gcagagtgcg gagttgtgtt atccgatgcg tcttcgcggt gatgaagccg tcgcattatt
    17101 gcagatgacg ccgtttgcct ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa
    17161 agaagtgttc gactgccaga cggactttaa tattcacctc tggcagcgtt cttattaacc
    17221 gtggaagtgc gtccagagga tctggacgcc gatgccgatc agcaccagcc cgccgagaat
    17281 ttccgctttt ttcccaataa ttgagccgat aaagcgacca accatcatcc ctaatgtcga
    17341 cataatcaag gttgcacaac caatggccaa tgcggtcgcg ataatgttga cctgaaggaa
    17401 agcaagacca acacccacag ccatggcatc caggctggtg gcaatcgcgg tggttaccag
    17461 tagccagaaa ccgtgccgac ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc
    17521 ctcaataatc attcgcccgc cgaggaatat cagcagcaca aacgcaatcc agtgattcca
    17581 ttcaaggaca aaccggctgg ctaacatgcc cattccccag ccgatcagcg gcgtcagggt
    17641 ttcgacggca ccaaaaataa ggccggttcg caatgcttca gaaaatttcg gtttatggag
    17701 gggggcacct ttaccgattg atgcagcaaa tgcatccatc gacataccaa acgcaagaag
    17761 aacagtagca gtgatattca tgacaacgtc ctgaccgggg tatccataaa acacatcatc
    17821 gcccccagca aacatgcata catgaacaac aagccgcgat gggttttcgc gttcatgcag
    17881 gcatagcgat tgatgtgcat atggtctcgc ctgactgaaa gtcttcagtc cgtacgtgcc
    17941 acgttttgca acgagtatgt tgacacgtac atttccggaa tctggaaatc ggctactccc
    18001 caaatgaggg cgcaacctta acatattttg agaatataaa acaactgttg gaatatatta
    18061 tttcattgct ttattgataa cgattttcat ttatgtttat atatcaatga aacgttaaaa
    18121 taaataacaa ataagggaag agataatata gcctaagcta tgtctgatta ttaaaaatac
    18181 actaaacata gctttggcta aattcataag ttattgagtt gatac
//
LOCUS       DBKEKI010000068        18055 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.068, whole genome shotgun sequence.
ACCESSION   DBKEKI010000068 DBKEKI010000000
VERSION     DBKEKI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.068"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..519
                     /locus_tag="ACUQPX_002420"
     CDS             <1..519
                     /locus_tag="ACUQPX_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HHV9057112.1"
                     /translation="PVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAID
                     DQKSFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSS
                     GQRTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTADKPGDAHASGWTL
                     AAKRGRDAGGGK"
     gene            516..842
                     /locus_tag="ACUQPX_002421"
     CDS             516..842
                     /locus_tag="ACUQPX_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HHV9057113.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            852..1202
                     /locus_tag="ACUQPX_002422"
     CDS             852..1202
                     /locus_tag="ACUQPX_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HHV9057114.1"
                     /translation="MAISAGRLTQMISILNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            1199..1645
                     /locus_tag="ACUQPX_002423"
     CDS             1199..1645
                     /locus_tag="ACUQPX_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HHV9057115.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            1642..1986
                     /gene="gp17"
                     /locus_tag="ACUQPX_002424"
     CDS             1642..1986
                     /gene="gp17"
                     /locus_tag="ACUQPX_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail completion protein gp17"
                     /protein_id="HHV9057116.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            2053..2769
                     /locus_tag="ACUQPX_002425"
     CDS             2053..2769
                     /locus_tag="ACUQPX_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HHV9057117.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVN
                     TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSA
                     EQPQSITSDACTVTVNGAGG"
     gene            2775..3149
                     /locus_tag="ACUQPX_002426"
     CDS             2775..3149
                     /locus_tag="ACUQPX_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HHV9057118.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            3173..3454
                     /locus_tag="ACUQPX_002427"
     CDS             3173..3454
                     /locus_tag="ACUQPX_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HHV9057119.1"
                     /translation="MRLALRLGRTLSELRHSLSASEAMMWMEFDRVSPLGDERGDIRN
                     AQIVKAVFGAQGMNVALKDAMLCWGEDEDKPEVDPFAALEDALSFAAQS"
     gene            3502..6561
                     /locus_tag="ACUQPX_002428"
     CDS             3502..6561
                     /locus_tag="ACUQPX_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HHV9057120.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGTLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALAKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERLRE
                     CMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQETFYAES
                     VLQEISKVLMNAAIVNGIRTAANSMSGAGGFIGSIGSWLGGAVANAKGGVYTSANLSA
                     YSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSP
                     VYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            6554..6895
                     /locus_tag="ACUQPX_002429"
     CDS             6554..6895
                     /locus_tag="ACUQPX_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHV9057121.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVAMVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            6895..7593
                     /locus_tag="ACUQPX_002430"
     CDS             6895..7593
                     /locus_tag="ACUQPX_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HHV9057122.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQSGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQLSELTAMTASFVLVTPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYMGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            7604..8347
                     /locus_tag="ACUQPX_002431"
     CDS             7604..8347
                     /locus_tag="ACUQPX_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HHV9057123.1"
                     /translation="MTQTESAILAHTRRCAPAESCGFVISTPEGERYLPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVLSHPGELPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMVVT
                     GFYRVPLSSAQPGDILLCCFGASVANHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWNTSAFTGIYNDLAAASACM"
     gene            8245..8925
                     /locus_tag="ACUQPX_002432"
     CDS             8245..8925
                     /locus_tag="ACUQPX_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HHV9057124.1"
                     /translation="MATTNAFCLASPPLEHICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRLQMNEGWYQIRIAGEDTAPEVVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSAMAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            9166..12645
                     /gene="gpJ"
                     /locus_tag="ACUQPX_002433"
     CDS             9166..12645
                     /gene="gpJ"
                     /locus_tag="ACUQPX_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage attachment tail tip protein J"
                     /protein_id="HHV9057125.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETVLGVEVTRAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPHRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GMQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQMVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTSSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDVATRTLTLD
                     REVTLPETGAATVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGAHFDGDQSGTLNSVIPPAVQHL
                     TVEVSAADGQYLAQVKWDTPRVVKGVRFSLRLTSGSGEGSHLVTTAITADTEHRFSGL
                     PLGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPT
                     VQFEFWFSEKRITNTAQVEKSARYLGTGSQWTVSGPHIKPGKDFWFYVRSVNLVGKSA
                     FVEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNE
                     ITQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDGIPYVAGIGAGIEDT
                     DGQPLSNILLLADRIAMINPENGNSTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPP
                     TFSLTPEGKLTARNADISGAITANTGTLNNVTINENCVIRGKLSANQIEGDLVKTVGK
                     AFPRNNSYASGTVTVTVYDDQGFDRQIIIPPVLFRGAKHQNFNSPNQQSYWYSTCKLQ
                     VLKNGVEIFHEPATDVSRVFSSVIDMPAGQGHVTLTFNVSSAGANNWTPTTYISDLLV
                     VVMKKSTAGISIS"
     gene            12714..13337
                     /locus_tag="ACUQPX_002434"
     CDS             12714..13337
                     /locus_tag="ACUQPX_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HHV9057126.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTVHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTCWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVEMVVIDLAYEGSGSGDWRTDGFIVGIGYKF"
     gene            13402..16215
                     /locus_tag="ACUQPX_002435"
     CDS             13402..16215
                     /locus_tag="ACUQPX_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HHV9057127.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSASEAATRATDAADSARAANTSAG
                     QAATSAQEAFSSAGTASAKASEASKSAAAAESSKSAAATSAGAAKTSETNAAASVTAA
                     EKSAESAASSASTATTKAGEATEQASAAARSASAAKTSETNAKASETRAESSKTAAAS
                     SASSAASSASSASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAA
                     TRAETAAKRAEDIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKAANDNAN
                     GRVPSNRKVNGKALTADITLTPKDIGTLNSVTISFSGGAGWFKLATVTMPQASSIVYI
                     ALIGGAGYNVVSPQQAGISELVLRAGNGNPKGITGALWKRTAVGLTNFAWINTSGDTY
                     DIYVEIGNYATSVNIHWDCTTNASVSIYTSPTYSASKPSSVTDGVVYTMYSSHQKPTP
                     SDIGALPTTGGTISGPLSVTDGITGALKGNADTATKLAAAPKINGVKFDGSADINLTP
                     ENIGAFARRSTGAYADSDGAVPWNAESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKA
                     HYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQG
                     QTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASST
                     DLGTKTTSSFDYGTKSSNATGDHNHSRGTMEITGTLGYFRSDSGNFYTASGAFTLGGS
                     APAHGFTGSQFTYGVPVNFNASRTWSGATSTTGNHAHSVPIGAHAHSVAIGSHGHTIT
                     VNAAGNAENTVKNIAFNYIVRLA"
     gene            16215..16799
                     /locus_tag="ACUQPX_002436"
     CDS             16215..16799
                     /locus_tag="ACUQPX_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HHV9057128.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPLDIPAGFVAVFNSDESSWHLFEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIRDAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            16772..16918
                     /locus_tag="ACUQPX_002437"
     CDS             16772..16918
                     /locus_tag="ACUQPX_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HHV9057129.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDGELSIVRVLNDFQPLPI
                     LRVN"
     gene            complement(16915..17163)
                     /locus_tag="ACUQPX_002438"
     CDS             complement(16915..17163)
                     /locus_tag="ACUQPX_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HHV9057130.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESANAWFVH"
     gene            complement(17225..>18055)
                     /locus_tag="ACUQPX_002439"
     CDS             complement(17225..>18055)
                     /locus_tag="ACUQPX_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9057131.1"
                     /translation="RTKRYVLELLMDSDISAIKLSELTENDVIEHCRLRNNAGAGPAT
                     VSHDVSYLGSVLDAAKPVYGINYTSNPAKSARPYLLKLGLIGKSNRRNRRPASDELDM
                     LIEGLQQRSTHKCSKIPFVDILKFSVWSCMRIGEVCRLRWEDLDQEQKSILVRDRKDP
                     RKKEGNHMKVALLGEAWDIVQRQPQKSEFIFPYNSTSVTAGFQRVRSKLGIKDLRYHD
                     LRREGASRLFEAGFSIEEVAQVTGHRSLNVLWQVYTELYPKSLHNRFEELQRSRNKIS
                     "
BASE COUNT         4247 a         4393 c         5539 g         3876 t
ORIGIN      
        1 cctgttccgc gtgatggtcg tgactacgat ccggatgttc tgcagaaggc ggttctggat
       61 gcggtgagtg ccctgccggc tccgcaggac gggcgtgatg ccacggcact ggaaatactc
      121 cccgccattg acgatcaaaa atcctttccc cggggcacgt atgccacaca ccaaggcgga
      181 ctctggcggg cgtatgaaaa aacgcacggg atgcggggat gggaatgcct ggttgacggg
      241 gtggcggata ttgacgtcag catgacgggt gaacggttgt tctctgtggt ggtccggcag
      301 agcagtggcc agcgtacgga aaaaacattt tccctgccgg tgatgctcta ccgcggtgtg
      361 ttcagagccg gtgaaaccta ccaccccggt gatacggtga cgtggggggg ctcgctgtgg
      421 cactgcaaca gtatgaccgc cgataaaccg ggggatgcgc atgcatctgg ctggacgctg
      481 gctgccaaac ggggacggga tgcaggaggc ggaaaatgac ggcattactg acactggaag
      541 agatcaaggc acatctgcgt gtcgaccatg acgcggatga tgacatgctg atggacaagg
      601 ttcgtcaggc taccgccgtg ctgctggcct acattcaggg cagccgggat aaagtgattc
      661 gtgaggacgg tgaactgatc ccgggcgagg cattaacccg gatgaagggg gctgccatgc
      721 gactgaccgg gatgctgtac cggaatccgg atcttgcgga gcgggaagaa ctgattcagg
      781 gggagctgcc gttttctgtt tccgtgctga tttacgattt gcgttgtccg acggtgttat
      841 gaggaggggg aatggcaata tctgcaggtc gtctgacaca gatgataagt attctgaacc
      901 cggtgttaac ccgtaatgct gccggagaaa tgacggaaga atgggtgtca tgcgggaaaa
      961 ttcatgcgga tatccgtggc aggagcagcc gggagcggat gcagtccggt gcggaaatgg
     1021 cgcaggcgga aatccgcatc tgggtgcgcg gtcagtccgg ccgggaaatc acggcagcgt
     1081 cacgacttca tgtgctgagt ggtccatggc gtgaccggat cctgaacgtt gtcgggctgc
     1141 ccgtgccgga tgcgaccggt gggcgtctgg aaattctctg tcggctggga ggggaaaaat
     1201 gatcgaaacc ctgctggatt tttcggggct ggaggacatc agccgcgatt tgcagcttct
     1261 gagtggtgcg gaaaataacc gggtgctgcg tgaggcaacc cgtgcggggg cgaatgtgct
     1321 gaaagaagaa gtggtgtcac gggcaccggt acgcaggggg aaactgcgcc gcaatgtggt
     1381 ggtcctttcc cggcgctccc gcgatggcgg gatggaatcc ggtgtccata tccgtggtgt
     1441 taatccggac accggtaaca gcgataacac catgaaggcg gataacccgc gcaatgcttt
     1501 ctactggcgg tttgtggaaa tggggaccgt gaatatgcca ccgcacccgt ttgtgcgtcc
     1561 ggcatttgat gtgcgcagtg aacaggcagc gcaggtggcg attgctcgga tgaaccgggc
     1621 cattgatgag gtactgagac gatgacggag gcggatttgt atcctcatct ggcgcatctt
     1681 gccggcgggc aggtgtaccc gtatgtggtc cccctgctgg atggcaggcc gtcggtggcg
     1741 cttccgtggg tggttttcag cctgatttca tcggtgtctg cggacgtgat gggcgggcag
     1801 gcggagtcct cagtgtcggt gcagatagac gtttatgccg ggactgtgac gcaggcgcgt
     1861 cagatacgtc aggacgcccg tgaagccata atgctgctgg ccccgggatc cgtcagtgaa
     1921 atgcaggact atattccgga aaaccgctgt taccgtgcaa ccctggagtt tcaggtcacg
     1981 gtgtgacttt ttcttttttt ctacaaaacc cataccccgc cgcgtgcggg ttttttatta
     2041 tcaggaggca gaatgtctgc tttgtatgaa cgctcacagc tgacgcaggt gatgatttca
     2101 tctgccccgg cgactgctga aactatggat aaggcggaat atctgcgcct ggactgcacc
     2161 atcaaggaag tccagttcac cgccggtcag aaacaggata ttgatgtgac cacgctctgc
     2221 tccacagagc aggagaacat caacggtctg ggggcgtcgt ccgagatttc catgtcgggt
     2281 aatttttatc tgaatcaggc ccagaacgcc ctgcgtgatg cctatgacaa tgacacggtg
     2341 tatgcgttta aggtgcagtt tccgtccggt aagggcttta agttcctggc ggaagtgcgt
     2401 cagcacacct ggtcatccgg taccaacggc gtggtggctg caacgttttc acttcgcctg
     2461 aagggtaaac cggtgtccta tgtggtaccg ctggcgtttg tgaaaaatct ggagaagaca
     2521 cttaccgtga ataccggtgc gctgctgaca atgtcagtca gtgtcaacgg gggaacgccg
     2581 ccttataaac acgcctggaa gaaggatggt cagccggtag agggacagac tactgacact
     2641 ttcagtaaag ccaatacgca gtcaggtgat aagggggctt atacctgcga ggtaacggat
     2701 tctgcagaac agccgcagag cattacctct gatgcgtgta cagtaacggt taatggtgcg
     2761 ggcggataag gcttatggca aaagatctga aaacactggc gctggccaga ctgtcagggt
     2821 tccgtcataa aacggtgaag gtgccggaat ggggaaatgt cagcgtggtg ctgcgggagc
     2881 cttcggcaga ggcctggtat ctgtggcagg aagtgctcaa tggtgatgga gaggatgacg
     2941 ataccctgtc ggtggtggcg aaaacccgcc gtaacctgga agcggatgtg acgctgttct
     3001 gcgatgtcct gtgtgatact gacctgcaac gggtgttcac tccggacgac cgtgagcagg
     3061 tgctggccgt ctatggtccg gtacatgccc ggttgctgcg tcaggcactg gaactgatcg
     3121 ctgatgcaga gtcggccaga aaaaagtagc ccgcccggaa attcgctttc tgatgcgact
     3181 tgcgctccgt ctggggcgca ccttatccga actgcggcac agcctgagtg cgagcgaggc
     3241 gatgatgtgg atggagtttg acagggtgtc cccgctgggt gatgagcgcg gggatatccg
     3301 taatgcacag atcgtgaaag cggttttcgg ggcacagggg atgaatgttg cactgaagga
     3361 cgccatgctc tgctggggcg aggatgagga taagccggag gtggatccgt ttgcggcgct
     3421 ggaagacgcg ctgagctttg cagcacagtc atgaatgatg agaaccgctg aggcggtttt
     3481 tttacgcccg gagaaaggtg aatggcgacg ttacgtgaac tgattatcaa aatttcggca
     3541 aattcacagt cattccagtc ggagatccag cgggcttccc gtatgggcag tgaatattac
     3601 cggaccctgc agaatggcgg gcgtcaggcc gctgcggcag cccgggagca gcgacgtgcc
     3661 ctggcagaac tgaacagcca gttgacggaa atccgcgctt cggctgtcgg aatggctggc
     3721 gcatttgccg gtgcctttgc caccggacac ctgatttcac tggctgatga gtggagttcc
     3781 gtgaatgccc gtctgaaaca ggcatcacag tcatccgatg aattttcgtc atcacagaaa
     3841 gtgctgatgg atatcagtca gcggacaggt accgcttttt cggataatgc ggccctgttt
     3901 gcccgttcgg ctgcctcgat gcgtgaatat ggttacagtg ccggtgatgt actgaaggtg
     3961 acggaggcca tttccacggg gctgaaaatc tccggtgcca gtacggctga ggcgggttcg
     4021 gtgatcaccc agttcagcca ggcgctggca cagggtgtgt tgcgcggtga ggaatttaat
     4081 tcggtcaatg aaagtggtga ccggatcatt cgtgcactgg ctgcaggcat gggcgtggcc
     4141 cgtaaagatc tgaaggcgat ggcggacgat ggtcaactga cggcggataa agtcgtgccc
     4201 gcgttaatca gccagctggg gacattgcgt gatgaatatg ccgccatgcc agaaacggtc
     4261 tctgacggga tcacaaaggt ggaaaacgcc tttatggctt gggtgggcgg tgcgaatgag
     4321 gccagcgggg tgacaaaaac gctctccggc atgctgaacg gtgttgccgg acagattgat
     4381 aatgtggcaa cagctgtggg tgcgctggtt gccgtcgggg tggcccggta ctttggcaat
     4441 atggcctccg gagcgatgtc tgccacggca ggacttgtga cggctgcacg taatgaagtt
     4501 gcactggcgg aagcacagtt caggggaacg cagattgcca cggcgcgggc aagggcagcc
     4561 gtgtaccgtg ctcagcaggc cgtggcggca gcccgcggga cggagatgca gattgctgca
     4621 gaggcccgtc tggcggccac acaggaacgc ctgaacagaa atattgctgc cagaagcgcc
     4681 gcccagaatg cgctgaacag tacaacggcg gtgggctcac gtctgatgag cggtgcgctg
     4741 gggctggttg gtggcgtacc cggactggtg atgctggggg ctgcagcatg gtacacgctg
     4801 taccagaatc aggagcaggc cagggagtct gcgcgccagt atgcactgac gatagatgaa
     4861 atcgcgcata aaacgccgtc aatgtctttg cctgaagcct cagataatga aggacgaaca
     4921 cgggcggcgc tgacagagca gaaccggctg attgatgaac aggccagtcg ggtgaaatcc
     4981 ctgcaggaaa aaatcgcagg atatcagtat gttctggcga acccgggctg gacgaccggt
     5041 gacggattca tgataaacca tctgacctcg gtgaagaccg taacggaagg gcttgctcag
     5101 gcaacagagc agcttgccgt tgagcagtcc cgtctggcac agatgcagga aaaagcgcag
     5161 tccattcagg atgtgcttgc cgggctggaa gaccgtcgtg tggcgttaat tcgtcagcag
     5221 gcggcagagc agaataaggt gtaccagtcc atgctggtta tgaacggtca gcatacggaa
     5281 ttcaaccgtc tgctggggct gggtaatgaa ctgcttcagc agcggcaggg actggtgaat
     5341 gtgccgttac ggctgccaca ggccactctg gatgataaac agcagagtgc cctggcaaaa
     5401 acagagcgtg agctggccct gtccagactg aaaggggaag aaaaagagcg tgtccgactg
     5461 gggtatgcgg cggatgacct cggttttgtg ggtgatccgt atcaggaggc gagacaacgt
     5521 tatatcagta atgccctgga agcctggcgc aataacgagg cgaataaacc caaatcccgg
     5581 ggtggaaaat cagagacgga aaaagcggaa gacagttttt cccggctgct gaagcagcag
     5641 aaagagcaat tggcacttgc ggggcagaat acagagctgg cgaagctgaa ataccagact
     5701 gcgcagggcg aactgaaaac cctgacggag atgcagaagc aggaactgct gcgcaatgcg
     5761 gccctgattg accagcaaaa aatccgggag cagttgcggt cccgggaaga gaccctgaag
     5821 aatgataatg tggctgcgcg tgcatcaaat gaagcggaac tgctggggta cgggcaggga
     5881 gagcggctcc gtgaatgcat gcgggagttg cagcagatcc gcgacagttt ccgccagaag
     5941 gatgcggacc ttcagtctca gtatcagacc ggggatatca gtgaggattt ttacagacag
     6001 gctctggcac agaacgcgca gtatctgagt gaacgtctta aagaccagga aactttttat
     6061 gccgaatcag tgctgcagga aatatcaaaa gttcttatga atgccgcgat tgtcaacggg
     6121 atcaggacgg cggcaaacag tatgtccgga gcgggaggat ttatcggcag tattggtagc
     6181 tggctgggcg gtgcggtggc caatgcaaaa ggcggcgtgt atacctcggc aaacctgagt
     6241 gcgtacagca acagtattgt ggatacgccc acgtactttg cctttgcaaa aggggcggga
     6301 ctgatggggg aggccggtcc tgaagccatt atgcccctga cccgggcggc ggatggctcg
     6361 ctgggtgtgc gagcggtggg cagtatgaac ggcagtgcag gtctggtgta ttccccggtc
     6421 taccatatcg ccattcagaa tgacgggact aatggccaga tagggccgga agctgcgggc
     6481 agccttgtgc aactgattga ccagcgggtg caggcggtga tgctgtccat gcgacgtgac
     6541 ggaggaatgc tgagtggctg agataaaaac gctgcatctg gtcccgcgtg aagggatgca
     6601 ggtgagtgag aaaccgtcgg tggcgatggt acggtttggt gacggttatg aacagcgccg
     6661 cccgacggga cttaatgccc gactgaagac gtttcaggcg gtgttccggg tgacggatga
     6721 ggcgacccgg cgatggctgg aagagttttt atcgtggcat ggtggttacc gtgccttttt
     6781 gtggcgaccg ccgaaacata accggacggt gagggtggta tgccgggagt ggagcgtcac
     6841 agataacgcc aggtacagtg atttcagttg tacgattgag caggtggtga actgatgcag
     6901 gatattcacg aagaaagtct gaacgagtcg gttaaatcag agcagtcacc gcgggtggta
     6961 ctctgggaaa tcgacctgac ggtacagagc ggtgagcggt attttttctg taatgagctg
     7021 aatgaaaaag gggagccggt cacctggcag gggcgtaagt atgaggcgta cccgattgat
     7081 ggcagcggct ttgaaatgaa cgggaaaggc agcagtgccc gcccgtcgct gacggtgtcc
     7141 aatctgtttg gccttgtcac cgggatggcg gaggatttgc agagtctggt gggtgccacg
     7201 gtggtccgtc gccgggtgta tgcgcgtttt ctggatgcgg tgaattttgt ggcgggcaat
     7261 ccggaagcgg acccggaaca ggaactgagc gaccgctggg tggtggagca gttatcagag
     7321 ctgacggcca tgacggcctc gtttgtgctg gtgacaccga cggagacgga cggggcgctg
     7381 tttcccggtc gcatcatgct ggcgaacacc tgtatgtgga cctaccgctc tgatgagtgt
     7441 ggttacatgg gcggggctgt ggcggatgag ttcgataaac ccaccacgga tatccgtaag
     7501 gacagatgca gcaagtgcat gcgcgggtgt gaactgcgca ggaatgtcgg caattttggc
     7561 ggtttccttt ccattaataa actttcgcag taaatcccgg tttatgacac agactgaatc
     7621 agcgattctg gcacacaccc ggcgatgtgc gcctgcggag tcttgcggct tcgtgataag
     7681 cacaccggag ggggagcggt atctgccgtg tgtgaatatc tccgcagagc cggaggcgta
     7741 ttttcgtatc gcaccggaag actggctgcg ggcagagatg cagggggaga ttgtggcact
     7801 ggtcctcagt catcccggtg agctgccctg gctgagcgag gctgaccggc ggctgcagat
     7861 aaaaagcgca ctgccctggt ggctggtctg ccggggggaa attcataaat tccgctgtgt
     7921 gccacatctg acaggacggc gctttgagca cggggtgacg gactgttaca cgctgttccg
     7981 ggatgcatac catctggcgg gaattgatat gccggacttt catcgcgagg atgactggtg
     8041 gcgcaacggc cagaacctgt acctggacaa tatggtggtc accggctttt accgggtgcc
     8101 cctgtcctct gcacagccgg gcgatatcct gctgtgctgc ttcggcgcat cggtggctaa
     8161 tcatgccgcc atttactgcg gcaacggtga actgcttcac catattcctg aacaactgag
     8221 taaacgggag aggtattccg aaaaatggca acgacgaacg cattctgtct ggcgtcaccg
     8281 ccactggaac acatctgcct tcacggggat ttacaacgat ttggccgccg cctcagcctg
     8341 tatgtgaaca cggcagcgga agccattcgc gccctgtcga tgcagatgcc gggctttcgc
     8401 cttcagatga acgaaggctg gtaccagata cgtattgccg gtgaagacac ggcaccggag
     8461 gtggtgtacg cccgccttca cgaacagctg ggtgagggaa cggtcatcca cattgtgccg
     8521 cgactggccg gggccggaaa gggtggactg cagattgtgt tgggggcggc agccatcgtg
     8581 ggctctttct tcactgccgg ggcatcaatg gcgttatggg gttcagccat ggcagccggt
     8641 ggtttttctg ccaccacgat gctgttttca cttggagcca gcatgattct gggcggtgtg
     8701 gcccagatgc tggccccgaa ggcaaaaaca ccggattacc gcgcaacgga taacggcaga
     8761 cagaacacgt acttttcctc gctggataac atgattgccc aggggaaccc gatgccggtg
     8821 ccttacgggg aaatgctggt tggctcccgc cgtatatccc aggacatcag cacccgtgat
     8881 gaaggcgggg gcggaaaggt cgtggttatc gggcggcagg gataaaacat aaaaaaatcc
     8941 cgcagtgatc gcggagctgc ggggacagac aaatgaagat caatgttaag gagttgtttt
     9001 tgttactcgg gcaaaaaaac actaacgcag cgaaattata agcgccacag tcagtgtgtg
     9061 aaaatgtgaa gatattcaga aattttattc cgtcatgacg caggcacccg gtgaggtgcc
     9121 tgttgttttt gtgagtgaac aattatcacg gtaagaggtg atgtaatggg caaaggtggc
     9181 ggcagggcgc acacaccgcg tgaggcgaaa gacaatctca aatccacgca gatgatgagc
     9241 gtgattgatg cgattggtga gggaccgata gaaggcccgg tgaaaggcct gcagagtatt
     9301 ctggtgaaca aaaccccgct gacggacacg gacggtaatc ccgtgataca cggtgtgacc
     9361 gcggtctggc gtgccgggga gcaggagcag acaccaccgg aaggctttga gtcctctggc
     9421 tctgaaactg tactgggtgt cgaagtgacc agggcaaaac cggtaacacg caccattacg
     9481 tcagcgaaca ttgaccgcct gcgggtgacc ttcggggtgc agtcactggt ggagaccacg
     9541 tcaaagggtg accgtaatcc gtcctctgtc cgtctgctga ttcagttaca gcgtaacggt
     9601 aactgggtga cggaaaagga tgtcaccatt aacggcaaga ccacctcaca gttccttgcg
     9661 tcggtgattc tggataatct gcctcaccgc ccctttaaca tccggatggt cagggagacg
     9721 gcggacagca ccacggacca gctgcagaac agaacgctgt ggtcgtcata caccgaaatc
     9781 atcgatgtga aacagtgcta cccgaacacg gccattgtgg ggatgcaggt ggatgcggag
     9841 cagtttggtg gtcagcagat gacggtgaac taccatatcc gcggtcgcat catccaggtg
     9901 ccgtcaaact atgacccgga aaaacgcacg tacagtggta tctgggacgg cagtctgaaa
     9961 ccggcataca gcaacaatcc ggcctggtgt ctgtgggaca tgctgactca cccgcgctac
    10021 ggcatgggaa aacgtctggg ggcggcggat gtggacaagt gggcgctgta tgccatcggg
    10081 cagtactgcg accagatggt gccggatggc ttcgggggca ccgagccgcg gatgaccttc
    10141 aatgcgtacc tggcacaaca gcgtaaggcg tgggatgttc tcagtgattt ctgctcggcg
    10201 atgcgatgta tgccggtatg gaacggtcag acgctgacgt tcgttcagga ccgcccgtcg
    10261 gatgtggtgt ggccgtacac cagcagcgat gtggtggtgg atgataacgg cgtggggttc
    10321 cgctacagct tcagtgccct gaaggaccgg cacacggcgg tggaggtgaa ttacaccgac
    10381 ccgcagaacg gctggcagac ctccacggaa ctggtggaag acccggaagc catactgcgc
    10441 tacgggcgca acctgctgaa gatggatgcg ttcggctgca ccagtcgcgg tcaggcccac
    10501 cgtgccggac tgtgggtgat aaagaccgga ctgctggaaa cgcagacggt ggatttcacg
    10561 ctcgggtcac aggggctgcg tcacacaccc ggtgacatta ttgaaatctg tgataacgac
    10621 tatgccggga ccatgaccgg cggacgtgtc ctgtccatcg atgtcgccac ccgcaccctg
    10681 acgctggacc gtgaggtgac actgccggag accggtgccg ccacggtgaa cctgattaac
    10741 ggcagcggta agccggtgag tgtggacatc accgcacacc ccgcgccgga ccggatacag
    10801 gtcagtaccc tgccggatgg cgtggagaca tacggtgtgt ggggactctc cctgccgtca
    10861 ttgcgccgtc gcctgttccg ctgtgtctcc gtccgggaaa acacggacgg cacctttgcc
    10921 atcacggcag tgcagcatgt gccggagaaa gaagccatcg tggataacgg ggcgcacttt
    10981 gacggcgacc agagcggcac cctgaacagc gtcatccctc cggcagtgca gcacctgacg
    11041 gtggaggtga gcgcagctga cggccagtat ctggcgcagg tgaaatggga cacgccgcgg
    11101 gtggtgaagg gcgtgcgctt cagtctgcga ctgaccagcg gaagcggaga aggcagccat
    11161 ctggtgacca ccgccatcac tgcggataca gagcatcgtt tcagtggtct gccgctcggg
    11221 gaatacaccc tgacagtcag ggcaattaac agttatggcc agcagggcga accggccacc
    11281 accaccttcc ggattaacgc gccagcaaaa cccgccacca ttgagctgac gccggggtat
    11341 tttcagataa cggcggtccc acgtcttgcg gtgtatgacc cgacggtaca gtttgaattc
    11401 tggttctcag aaaaacgcat cacgaacaca gcacaggtgg aaaaatctgc ccgttatctg
    11461 gggaccggca gtcagtggac tgtctccggc ccgcacatta agcccgggaa ggatttctgg
    11521 ttttatgtac gcagcgttaa cctggtgggg aaatctgctt ttgttgaagc cagtggacgg
    11581 gccagcaatg atgcagaagg gtatctgggg ctgtttcggg aaaaaatagg aaaactgcat
    11641 ctggctcagg ggctgtggga gctgatagac aacagccagc ttgcggatga gatggcggag
    11701 atgaagacca ccatcaccga aacccgcaat gaaatcacac agacggtcag taaaacgctg
    11761 gaagaccaga gcgccaccat tcagcagata cagcgcgtgc agaaggacac aaatgatgac
    11821 ctggctgcgc tgtacatgct gaaggttcaa aaaacgaaag acggcattcc ctatgtggcc
    11881 gggattggtg cagggattga ggatactgat ggccagccac tgagcaacat actgctgctg
    11941 gctgaccgta tcgcgatgat aaatccggag aacggcaaca gcacgccgtt atttgtggcg
    12001 caggggaatc agctgttcat gaacgacgtg ttcctgaagc gactgtttgc ggtgagtatc
    12061 acgtcatccg gcaacccccc gacgttttcc ctgacgccgg aagggaagct gacagccagg
    12121 aacgcggata tcagcggagc aattaccgcg aataccggca cgctcaataa tgtcaccatt
    12181 aacgagaact gtgtcatcag agggaaactg tctgcaaacc agattgaagg cgacctggtg
    12241 aagacggtgg ggaaagcctt tccccggaat aacagttatg ccagcgggac ggtaactgtc
    12301 acagtttacg atgaccaggg attcgaccgg cagattatca ttcccccggt gctgtttcgc
    12361 ggggcgaaac accagaattt caacagcccg aatcagcagt cgtactggta ttccacctgt
    12421 aagctgcagg tgctgaagaa cggggttgag attttccatg aaccggcaac ggatgtcagc
    12481 cgggtgttct catcggtgat agatatgccg gcagggcagg gtcatgtcac cctgacgttt
    12541 aatgtgtcgt cggccggtgc gaacaactgg acgccgacaa cgtacatcag tgatttactg
    12601 gttgtggtca tgaaaaaatc cacggcaggg atcagtatca gctgacggtt tattaacccg
    12661 gacgggcacc tcaggaggag ccttttttat tgactgaaaa caaagaggta atcatgcggc
    12721 atttatacgc aacgatatta ttgtttacta ccctgctggc agggattgcc tttcctgcac
    12781 aggctgaaag cggacacggt gcattttccg tgggatatgc tcaggttcac ccgggcggcg
    12841 taccggcatt gtccggtacc ggtgcgcgtg caggtgattt aaaagggatt aatgtgaaat
    12901 accgttatga gttcacggtt cacctgggcg gcattgtcgc gctgagttat gcatcggtga
    12961 agaaaagtga cacgatgaag acgggtgaaa ataccttcca ttatgaaagc ctgcgcggtc
    13021 gttatgtcag tctgatggcc ggccctgtct ggcagctcag tgagcgggtc agtctctatg
    13081 gcatggccgg gatggcgtac acctgctggt ctgacagtgt tcaggattac cggcgtgatg
    13141 aagtaaaacc ggggtatgtg aaggagacca ccaccgccag tgatggtcat actgcgcgtc
    13201 atctgtcgcc ggcctggaat gccgggattc agttcagtcc cgtagagatg gtggttattg
    13261 accttgctta tgaaggttcc ggcagtggcg actggcgcac tgacggtttc atcgtgggta
    13321 tcggttataa attctgatta gccaggtaac acagtgttat gacagcccgc cggttcaggc
    13381 gggctttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa agacggcaca
    13441 ggaaaaccgg tacagaactg cacaatccag ctgaaagcaa aacgtaacag caccacggtg
    13501 gtggtgaaca cgctggcatc tgaaaatccg gatgaagccg ggcgttacag catggacgtt
    13561 gagtacggtc agtacagcgt tattctgttg gtggaagggt tcccgccgtc acatgccggg
    13621 accatcaccg tgtatgaaga ttctcaaccc ggtacgctga atgattttct tggtgccatg
    13681 actgaggatg atgtccgtcc ggaggcactg cgccgttttg aactgatggt ggaagaggtg
    13741 gcgcgtaacg cgtccgcagt agcacagaac acggcagccg cgaagaagtc agccggcgat
    13801 gccggcacat ctgccagtga ggcggcaacc cgtgcgactg atgctgcaga ttcagcacgt
    13861 gcagccaaca cgtcagccgg acaggccgcg acgtcggctc aggaggcttt ttccagcgca
    13921 ggaacggcat cagcaaaagc ctctgaggca tcgaaaagtg ctgctgctgc agagtcctca
    13981 aaaagcgcgg cggccaccag tgccggtgca gcgaaaacgt cagaaacgaa tgcggcagcc
    14041 agcgttaccg ccgctgaaaa atcggcagaa agcgccgcat cgtccgcttc aacagccaca
    14101 acgaaggctg gcgaagccac tgaacaggcc agcgcagcag cgaggtctgc ttccgcagcg
    14161 aagacatccg aaacgaacgc gaaagcgtcg gaaacccgtg cagaatcctc aaaaacggct
    14221 gccgcatcat ccgccagttc ggcggcgtca tcggcatcat ctgcgtctgc ttcaaaagat
    14281 gaggcgacca gacaggcgtc agcagcgaag ggcagcgcca cgacggcatc cacaaaggcg
    14341 acagaggctg ctggcagtgc gacagcggca gcacagagca aaagtacggc agaatctgca
    14401 gcaacgcgcg ctgagacagc ggcaaaacgg gcagaggata ttgcatccgc cgtggcgctt
    14461 gaggatgcga gcacgacgaa aaaggggata gtccagctca gtagtgcaac caacagcaca
    14521 tctgaaacac ttgccgcgac accgaaggca gttaaagcag cgaatgacaa tgcgaatggc
    14581 agggtaccat ctaaccgaaa agttaacggg aaagcactga ctgcggatat aacattaaca
    14641 ccgaaagata ttggcacttt aaattcagta acgatctctt tctctggcgg ggcagggtgg
    14701 ttcaaactgg caacggttac catgccacaa gcgagttcca tcgtttacat cgcattgatt
    14761 ggtggcgctg gttacaacgt cgtctcccca cagcaggcag gcatttcaga actggttcta
    14821 cgagcaggca atggaaaccc caaagggatt accggagctt tgtggaagcg tacagccgtc
    14881 ggattaacga atttcgcctg gatcaacaca tccggcgata catatgatat ttacgttgag
    14941 attggcaatt atgcgacgag tgtaaatatc cattgggatt gtactacaaa tgcgtcagtt
    15001 tctatttata cctcgccaac atattcagcg agtaagcctt ccagcgttac cgatggtgtt
    15061 gtttatacga tgtatagctc acatcagaaa cctacaccat cagatattgg agcgctgcca
    15121 acgactggag ggactatttc aggtccgttg tctgttactg atgggatcac cggggcactg
    15181 aaggggaacg ccgataccgc gacgaaactt gcggcagccc caaaaattaa cggtgttaag
    15241 tttgatggct cggcggatat taacctcacg ccggaaaata ttggtgcatt tgcccgacgt
    15301 tcgacggggg cttatgcgga ttcggatgga gccgttccct ggaatgccga atcaggcgct
    15361 tacaatgtca cccgctctgg cgacagctat attctggtta acttctatac cggagtcgga
    15421 agttgccgga ccttgcagat gaaggcgcat tacagaaatg gtggtctgtt ctaccgttct
    15481 tcaagagacg gttatggttt tgaggaagac tgggcagaag tttatacctc gaaaaatctt
    15541 ccaccagaaa gctacccagt cggcgcacca atcccgtggc catcagatac cgttccgtct
    15601 ggttatgccc tgatgcaggg gcagactttt gacaaatctg cctacccgaa acttgcagcc
    15661 gcttatccgt caggcgtgat ccctgatatg cgtggctgga cgattaaggg caaacctgcc
    15721 agtggtcgtg ccgtattgtc tcaggaacag gacggcatta aatcgcacac ccacagcgcc
    15781 agtgcatcca gtacggattt ggggacgaaa accacgtcgt cgtttgatta tggtactaag
    15841 tcttcaaatg ccactggtga ccataaccac agccgaggca ctatggaaat taccggtact
    15901 ctcggttact tcagaagtga tagcggtaat ttctatacag caagtggagc atttacactg
    15961 ggtggctctg cgccagccca tggatttacg ggctctcaat ttacttatgg tgttcctgta
    16021 aactttaacg cttccagaac ctggtctggt gccacaagca ctacaggtaa ccatgcgcac
    16081 tctgttccaa ttggtgctca tgcgcactcc gttgcgattg gttcacatgg acacaccatc
    16141 accgttaacg ctgctggtaa cgcggaaaac accgtcaaaa acatcgcatt taactatatt
    16201 gtgaggcttg cataatggca ttcagaatga gtgaacaacc acggaccata aaaatttata
    16261 atctgctggc cggaactaat gaatttattg gtgaaggtga cgcatatatt ccgcctcata
    16321 caggtctgcc tgcaaacagt accgatattg caccgttaga tattccggca ggcttcgtgg
    16381 ctgttttcaa cagtgatgag tcatcgtggc atctctttga agaccatcgg ggtaaaacgg
    16441 tttatgacgt ggcttccggc gacgcgttat ttatttctga actcggtccg ttaccggaaa
    16501 atgttacctg gttatcgccg gaaggggagt ttcagaagtg gaacggcaca gcctgggtga
    16561 aggatacgga agcagaaaaa ctgttccgga tccgggatgc ggaagaaaca aaaaacaacc
    16621 tgatgcaggt agccagtgag catattgcgc cgcttcagga tgctgcagat ctggaaattg
    16681 caacggagga agaaacctca ttgctggaag cctggaaaaa gtatcgggtg ttgctgaacc
    16741 gtgttgatac atcaactgca cctgatattg agtggcctac gaaccctgtc agggagtaat
    16801 cattgggatt atgccgcagc acgtcttaag caagaacgtg ctgcggttgg atggtgaact
    16861 ttcgatagtg cgagtattga atgatttcca gccgttaccg attttacgtg ttaattagtg
    16921 aacaaaccac gcgttagcag actcccaggt atctttcaga gtttcctgaa caaaagtttt
    16981 agctgaatct ttatcggcgg tgcgcgtaac agaaagacca tcgttgctgg tagcttttac
    17041 gatcacctct acatcgtcat aacgtttact gatgcgtcgg gttaattctt cctttaacgc
    17101 atccacagca ccggttggca ttttagtcat tttttctttg gctatgcaga tttcaatacg
    17161 cataaaagtc cctctatact gtgtttgtat acagtattat ttttaactgt atggataaac
    17221 agtgtcaaga gatcttattt ctgctccttt ggagctcttc aaaacgatta tgtaaagatt
    17281 tcggatacag ttcggtatat acctgccata gcacgtttaa tgaacgatgc cctgtaacct
    17341 gggctacttc ctcaatacta aaaccagcct caaataagcg acttgcccct tctctacgca
    17401 aatcatggta tcgcagatcc ttaataccta atttgcttct taccctctga aatcccgcag
    17461 taacagaagt gctgttatat ggaaaaatga attccgattt ttggggctgt cgttggacga
    17521 tatcccaggc ttccccaagc aaggctactt tcatgtggtt gccttccttt ttgcgtggat
    17581 ctttcctgtc tcttacgagt atagattttt gttcctggtc gagatcttcc catcgtaacc
    17641 ggcatacttc tccgattcgc atacaggacc acacagaaaa tttgaggata tcaacgaacg
    17701 gaatttttga gcatttatga gtagatcgtt gttgaaggcc ttcaatgagc atgtccagtt
    17761 catcagatgc tggtctacga ttacgacggt ttgatttacc aatcaaacca agtttaagta
    17821 ggtatggacg agcacttttc gccgggtttg atgtgtaatt aattccgtat acaggtttgg
    17881 ccgcatccag aacactgcca agataactaa catcgtggct gactgttgct ggacctgcgc
    17941 cagcgttgtt tcttagcctg caatgttcaa ttacgtcatt ttctgtcagt tcagatagtt
    18001 tgatcgcgga gatgtcacta tccataagca gttccagcac atatctttta gtacg
//
LOCUS       DBKEKI010000069        17957 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.069, whole genome shotgun sequence.
ACCESSION   DBKEKI010000069 DBKEKI010000000
VERSION     DBKEKI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.069"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            121..1350
                     /locus_tag="ACUQPX_002440"
     CDS             121..1350
                     /locus_tag="ACUQPX_002440"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9057132.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGSYPDLSLKAAREKAAQCRTWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWMERLGI
                     LAGTHENVTTLPVARRK"
     gene            1725..1913
                     /locus_tag="ACUQPX_002441"
     CDS             1725..1913
                     /locus_tag="ACUQPX_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9057133.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            1971..2564
                     /locus_tag="ACUQPX_002442"
                     /pseudo
     CDS             1971..2564
                     /locus_tag="ACUQPX_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            2718..2915
                     /locus_tag="ACUQPX_002443"
     CDS             2718..2915
                     /locus_tag="ACUQPX_002443"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HHV9057134.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGSHHA"
     gene            2908..3372
                     /locus_tag="ACUQPX_002444"
     CDS             2908..3372
                     /locus_tag="ACUQPX_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HHV9057135.1"
                     /translation="MRKNRLQKIITGLYASPVAHMATGFSSLNTSGGQPPTSDKRFFC
                     VRKPTHTRIMAGRNGGAFVLAGFQMTSLLTLLRLATMFSSVVARLLKIHLGAFHMAVS
                     ARPYFVWRFMQCLTDSIAIFTVTAATEREAPAQLPHAHLIFVARIRQEVRHV"
     gene            3365..3583
                     /locus_tag="ACUQPX_002445"
     CDS             3365..3583
                     /locus_tag="ACUQPX_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057136.1"
                     /translation="MFEVLIVITRYHNEAVMSRREINTYRYKSRRTAVKKFGQIAEEI
                     KNDLYRVRQVKGNPCKYVSSVELRYVAE"
     gene            3573..4021
                     /locus_tag="ACUQPX_002446"
                     /pseudo
     CDS             3573..4021
                     /locus_tag="ACUQPX_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688634.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4014..4247
                     /locus_tag="ACUQPX_002447"
     CDS             4014..4247
                     /locus_tag="ACUQPX_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057137.1"
                     /translation="MRDIYLETIDRASLALSHSESMMEILRMCLESFGDNERNAKKTR
                     IITSLITLLEPVINELQEIETLHDRYNEQHTGE"
     gene            4253..4552
                     /locus_tag="ACUQPX_002448"
     CDS             4253..4552
                     /locus_tag="ACUQPX_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HHV9057138.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFDRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            4549..5949
                     /locus_tag="ACUQPX_002449"
     CDS             4549..5949
                     /locus_tag="ACUQPX_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HHV9057139.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQEGRLAGDN
                     VPPVWLGEQQLAELTDMRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVQDANYY
                     PDGMHSQKRENWREYLERERGKKPGEEKHHQRKTTLPMSVGSAGYDAQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPRPPV
                     ELERIEEAGNKTENHAALWQCIRTRTAHGEACTIALLRDDMKKLGYEMKHFRRWLYKL
                     ENDGVIVIDGDDVRPL"
     gene            6151..6402
                     /locus_tag="ACUQPX_002450"
     CDS             6151..6402
                     /locus_tag="ACUQPX_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057140.1"
                     /translation="MNRKTRDKTAPKYKALDMTELSLKVAIRTIDRHVGEGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            6399..6704
                     /locus_tag="ACUQPX_002451"
     CDS             6399..6704
                     /locus_tag="ACUQPX_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057141.1"
                     /translation="MSEPKFGEKLYKHNGRITIQQISAATPGWWVETDEGSSTVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            6714..7124
                     /locus_tag="ACUQPX_002452"
     CDS             6714..7124
                     /locus_tag="ACUQPX_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HHV9057142.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAAPLPCSQADDGT
                     ATMWLSVLAFGRQAESLARHRKGELLTVAGNMQISQWTGQNGETRQGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGDDDPYGDDIPF"
     gene            7137..7409
                     /locus_tag="ACUQPX_002453"
     CDS             7137..7409
                     /locus_tag="ACUQPX_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HHV9057143.1"
                     /translation="MVHDRIAEELEAKGFYRRASARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRPPEPPDNYGDLKKAVDRTYAEMGMDGAGDEIWRNYQDR"
     gene            7698..8855
                     /locus_tag="ACUQPX_002454"
     CDS             7698..8855
                     /locus_tag="ACUQPX_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HHV9057144.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETDVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            8895..9467
                     /locus_tag="ACUQPX_002455"
     CDS             8895..9467
                     /locus_tag="ACUQPX_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HHV9057145.1"
                     /translation="MGEKIMKSMEIRSSEITTSASNTLTGYVVRWDNLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            9469..10680
                     /locus_tag="ACUQPX_002456"
     CDS             9469..10680
                     /locus_tag="ACUQPX_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HHV9057146.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPVALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            10677..11015
                     /locus_tag="ACUQPX_002457"
     CDS             10677..11015
                     /locus_tag="ACUQPX_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HHV9057147.1"
                     /translation="MNIGRLRDRVTIQTLKQTRDITGEILETWEDGHTLWASVNMISS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            11012..11308
                     /locus_tag="ACUQPX_002458"
     CDS             11012..11308
                     /locus_tag="ACUQPX_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HHV9057148.1"
                     /translation="MTEELITLEEVKLHCRIDGDEEDQLISGYIAASLEACQIHIGRR
                     FDDGLEFTPAIKIGCMMFIAHLYENRQLVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            11308..11748
                     /locus_tag="ACUQPX_002459"
     CDS             11308..11748
                     /locus_tag="ACUQPX_002459"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HHV9057149.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFHEEEERAANRNNWMYDADE"
     gene            11732..11914
                     /locus_tag="ACUQPX_002460"
     CDS             11732..11914
                     /locus_tag="ACUQPX_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057150.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRGREVMECFRNR"
     gene            12037..12393
                     /locus_tag="ACUQPX_002461"
     CDS             12037..12393
                     /locus_tag="ACUQPX_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HHV9057151.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            12377..14038
                     /locus_tag="ACUQPX_002462"
     CDS             12377..14038
                     /locus_tag="ACUQPX_002462"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140657 - ATP-dependent activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HHV9057152.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGATPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            14052..14333
                     /locus_tag="ACUQPX_002463"
     CDS             14052..14333
                     /locus_tag="ACUQPX_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057153.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(15053..15196)
                     /locus_tag="ACUQPX_002464"
                     /pseudo
     CDS             complement(15053..15196)
                     /locus_tag="ACUQPX_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
     gene            15331..15501
                     /locus_tag="ACUQPX_002465"
     CDS             15331..15501
                     /locus_tag="ACUQPX_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057154.1"
                     /translation="MFNYRGQLSSAMTRSNTADNCNIMDNLFRTLFSTFTHLRTSSTI
                     LLVGEQHWRNAL"
     gene            15608..15973
                     /gene="mdtJ"
                     /locus_tag="ACUQPX_002466"
     CDS             15608..15973
                     /gene="mdtJ"
                     /locus_tag="ACUQPX_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HHV9057155.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            15960..16289
                     /gene="mdtI"
                     /locus_tag="ACUQPX_002467"
     CDS             15960..16289
                     /gene="mdtI"
                     /locus_tag="ACUQPX_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HHV9057156.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(16328..17149)
                     /locus_tag="ACUQPX_002468"
     CDS             complement(16328..17149)
                     /locus_tag="ACUQPX_002468"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine peptidase"
                     /protein_id="HHV9057157.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(17249..17332)
                     /locus_tag="ACUQPX_002469"
     CDS             complement(17249..17332)
                     /locus_tag="ACUQPX_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057158.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(17425..17733)
                     /gene="asr"
                     /locus_tag="ACUQPX_002470"
     CDS             complement(17425..17733)
                     /gene="asr"
                     /locus_tag="ACUQPX_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HHV9057159.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
BASE COUNT         4773 a         4207 c         4878 g         4099 t
ORIGIN      
        1 ttttgattat caacagattt gtgattttcc ttgttccagc ttgtttcacg ttgtagccct
       61 ttgtgcaacg atcggtgtag tcactggtgt agtcactttt tcgtaaagag ggggtaaaac
      121 gtgagcagag caatgaacaa actgagcgat accttgctaa ggaaacttca tggtaagcca
      181 gcggagaaaa acacgtttta tagtgatggc ggaaacctga gcgtgaaata tttaacatca
      241 gggaaattga cctggtattt cacatacagg gccggaacgg gaagggagac acgacccgaa
      301 cgcattaagc tgggaagtta tcctgatctg agcctgaaag cagccaggga aaaagccgca
      361 cagtgtcgca catggctggc tgaggggaaa aatccacgtc atgagatgaa ttacaccgta
      421 caggaagcgt taaagcccgt aacggttggc gatgcgctca cctactggct tgagtattac
      481 gtaaaggaaa accgcgtgga ttatatcgcc ctgaaaaggc gacttaataa tcacgtaata
      541 cagcagattg gtgctatgcc gctggataaa tgcgagctac ggcactggct ggcctgtttt
      601 gaccaggtgg caaagcgaac gcctgttact gccggattcg tgctacaggc gtgcaaacag
      661 gcgcttaagt tctgccgtag gcggcgctat gcaatcagca acgttctgga cgatctgaac
      721 gtggcggacg ttgggaaaaa accggatata agcgaacgtg tcttaagtaa caaagaactt
      781 ggcgaattat tgcaggcact ggacaaaaaa atattttccc cttactacgt cgcgttaatc
      841 cgcctcctga ttgtcttcgg agccaggacg gtagaactga ggctatcgga gatcggggag
      901 tgggatttta cggaaatgct atggaccgtt ccgaaggagc acagcaaaac gaaggtcgca
      961 atattccggc ccataccgga agcgatcctg ccgttcgtca cgcagctggt ggagcagaac
     1021 aggcacacgg gcttattgct gggggaagtg aaacaggagg ccagcgtatc gcagtatgga
     1081 agattagcgc acaggaggct taatcatcct cactggtcac tgcatgacat ccggcgcacc
     1141 tttacaacca tgctgaacga tttaggcgtt gatccgcatg tcgtggagca gcttacaggc
     1201 caccagatgc caggaatgca gcgagtttat aatcattccc gttatctgga tgctaaacgc
     1261 aatgcgctgg atatgtggat ggagcggtta gggatactgg cgggaacaca tgaaaacgta
     1321 accacgctac cagtagccag aagaaaataa tttttttcgt attttttcag tatgcgcata
     1381 ctggatatgc gaacagatac aacgtgcaac aatgagcaac aatgcggaac aactacgaac
     1441 aagaggcgaa aaagtgtaca gatttataag tagctgattt ttaatgtgtt actggttttt
     1501 tatacactag cgaatcactc tttaaatagc gagaaaaaag gcatgaaaca atattttatg
     1561 cctttgtatt aacatgaatt taaatgattt tatcctgatt attcctcttt ctgtaaacca
     1621 tacggcctca acatttgcca actctgaatc atcctgacac aatcagacac atctcaacgc
     1681 aatagaacac gaaataactc tttaagaaac gaaagggggc aatagtgtta agcactgatc
     1741 ggtttatacg tgaaaaagaa tgcgaaaaac taaccggcct tagccgtacg tgtcgctacc
     1801 gcctggaaaa ggccggacaa ttcccatcac gtcgtaaact tggcggtcgt tccgttggct
     1861 ggtctttatc cgaggttctg gcctggaagg atagctgcaa ggcagttcat taatcacgct
     1921 ggcggcacac agccgccaca catcaatcat ctgaacacag agctataacc atgaagattg
     1981 aatatacgcc agaacgtggg cggggattcg ttcgccctgg tgagactgga aaaccacaaa
     2041 attggggttt ttcaggcata aaaaaagcgg ccccgaaatg gagccgcctt tctgaacaga
     2101 taacccgctg cgccgtttgt gtgtgtgatc ccaaacataa gcacggggat gatagccgct
     2161 atcaggctgg tgggcaatgc aatcagtctg gttcagttcg ttgccatacc tgcaatgagc
     2221 gcttttccct gtactcttta aggaattgct caagggcaaa agcacatggc gcgaatcttt
     2281 ctgattcatg ttctatcttt ctgcgccgtc ttttccgtgc cggtgataat gttttggtca
     2341 attctttatc ggtcattgtg ttgtcctgca tagcaatgcg ccgtaatacc tcacaccacg
     2401 gcgctgatag tttttatcct ttgggttcta tgccgcgttg ctgtaattct ttgcggagaa
     2461 cgcgtttaat ccaagtggct aaagatgtgt cgccatcggc cttagctgct tcttctagct
     2521 gcgctctgaa gtcttccggt aatcgcattt ggtatggagg cgaacgtttt tctaatggtg
     2581 ttgacatggt aattaccctt cattactata cctacatggt aattaccatt gtacttgcca
     2641 ttacacaaaa atcaacgccc cgcagtgctg ggaacacata cggggcgtct aaccaacaac
     2701 gtaaactagg agccgttatg gttgccgtaa atcatatacc acaccttgta cacacacaaa
     2761 cggcctttgt gtggcgtttt ctggcactga gtgccggaga atctcaaatc atccacgtaa
     2821 ccgcctggac ggaacgcgaa gcgcgtagcc gttgcccgtc cggttgtgtt gctgtattcg
     2881 ccgcccgtat tcgtcagggg tcacatcatg cgtaaaaacc gcttgcaaaa aattatcacg
     2941 gggctgtatg cttcccccgt cgcccacatg gcgaccgggt ttagcagcct gaatacatct
     3001 ggcggacagc cgcccacatc cgataagcgg tttttttgtg tccgtaaacc tacccatacc
     3061 cgcattatgg cggggcgtaa cgggggagcc tttgtgctcg ctggtttcca gatgaccagt
     3121 ctgctaaccc tgttacgtct cgccaccatg tttagcagcg tagtagcgag actccttaaa
     3181 attcatctgg gagcctttca catggctgta tccgcacgcc cttactttgt ctggcgcttt
     3241 atgcagtgcc tgacagacag cattgcaata ttcaccgtta ccgctgccac tgagcgcgaa
     3301 gcacccgcac agctgccgca cgcacatctg atttttgtcg cccgcattcg tcaggaggtg
     3361 cgccatgttt gaagtgctga tagtgataac cagatatcac aatgaagcgg taatgagcag
     3421 aagggagata aacacttacc gctacaagag ccgccgtaca gcggtaaaaa agtttgggca
     3481 aatagcggaa gagattaaaa acgatctcta ccgtgtgcga caggtaaaag gaaacccgtg
     3541 taaatacgta agttcagtgg agttaagata tgttgcagaa taaaaccaca ccggacgcag
     3601 cagccgccgc actcactacg ctgatgcacg cgcttattga tattgaatgc acggcagagc
     3661 ttgcgcaggg agaagaacag aaagaccgga cacagttcgc cctggaatgt atccgataca
     3721 tcgcaacgcg gtcactgaat gacgctaaaa atattcttgt tgccgattgt gaaaatgggg
     3781 gggggttatg cgtgatgatc gttttaattc cctgaaacag gaattttccg gcgttcctga
     3841 tgatgcggct gatgcgcttt cgtcaatgcc agaacttatt agagcggctt ttttcttact
     3901 ttccacgaaa gaatataaat caacgggact tgatgtactg aatatcgccg ccgattatgc
     3961 ggaatatgtg gcagaggcgc gttacagaag aaaacttcct gaggatgtaa gccatgcgtg
     4021 atatttatct tgaaacaata gaccgtgcat cacttgcact ttctcatagt gaaagcatga
     4081 tggaaatatt gcggatgtgc cttgaatcct ttggcgataa tgaacgtaat gcaaaaaaaa
     4141 cgcggattat tacatcatta ataacgcttc ttgagcctgt aataaatgag ttgcaggaaa
     4201 tagagacatt gcacgacaga tataacgaac agcacaccgg agaataaaaa taatgaaact
     4261 taaatattct ggcttaactg ccagtggcaa cactcaccct aaatttacgc gcggtgatat
     4321 ttaccgcgac cagtacggcg gcacggtaat gattaagggc gtggaagaac ggcgcgtaac
     4381 ctaccgccgt gaaggttacg aatatgattg cgtgatgcct gtttatcagt tcgatcgtga
     4441 tttttctctg gtacagaccg cgccgcataa cgtgcccacc agcaacgcca gggcacgggc
     4501 aaacatccag aagctgaaaa ccatgattaa cggattcagg ggcaagaaat gaaaagcgca
     4561 ccgaacttaa aaaaacagcc ttacgacaaa atgaccgaag tcattatttt tgcgggtagt
     4621 gatgcctggg cacacgcgaa gcagtggcag gagcaggagg gccgacttgc tggcgataat
     4681 gtgccacctg tatggcttgg ggagcagcaa cttgcagaac tgacagacat gagaatcatc
     4741 gacgagggcc gctattgcgt ccggctgtac aaagcaggcc atatcagacc atcaaatatt
     4801 aatgccattg cgcacaagct ggcagcggca ggtgtacagg atgcgaatta ctaccccgat
     4861 gggatgcaca gccagaagcg ggagaactgg cgcgaatatc tggaacggga gcgggggaaa
     4921 aagccagggg aagaaaaaca ccaccagcga aaaaccacgc taccaatgag cgtgggatct
     4981 gccggatacg acgcacaact tgattacgtg gttaagggga ttattccggc ggtatcgcta
     5041 tgcagcatat acggggcaag cggttcctat aaatcattcc ttgcgggttc gtgggcgtgt
     5101 cacgtttcca cgggccgcca gtggggaggt agaagggttg ctcatggggc ggttctctat
     5161 gtggttggtg aaggcggtat aggtgttccg cgtcgtgtaa aagcctggga ggttgtgcac
     5221 gatgagcagg tgaaaaatct gtatctggta aaccgcccca tctttccggc tgccccgctt
     5281 gatgttgatg aaatggttat cgctgcccgt caggtggagc gggaaacggg taaacctgta
     5341 cgcatgatta ttctggatac gctggcgcgt tgctttggtg ggaatgatga aaatgattcc
     5401 cgtgatatgg gggcgtttat ccgtggttgt gacgaactga aacgacgcac aggggccacg
     5461 gtgctggtgg ttcaccattc cggcaaggat gagacgaaag gcgcgcgcgg ttccagtgca
     5521 tttcgtgctt cgctggatgc tgaataccgg atacgcaggg aggacgcagg aagcgaagcg
     5581 ctggttatct catgcaccaa aatgaaggac gcggaggaac tcaaagaagc cgcatatgac
     5641 ttacgcgtgg tggagctttt taccgacgct gacggggagt taatcacgtc gctggtggtg
     5701 gtggataagc cgcgccctcc cgttgaactg gagcgcatcg aggaggcagg gaacaagacg
     5761 gagaatcacg ccgcgctatg gcagtgcatc agaacacgaa cagcacacgg ggaggcgtgc
     5821 actatcgccc ttctccgcga cgatatgaaa aagctggggt atgagatgaa gcacttccgg
     5881 cgctggctgt acaagctgga aaatgatggc gtaatcgtta ttgacggtga tgacgtgcgc
     5941 ccgctgtaaa aagtggggag taaaagtggg gagagtgggg aatttaacaa aattgaaaca
     6001 taattaccca ctttcccacc tgtatatacc caaaaaagtg gggagtaaaa aatatattga
     6061 aaaacatcac gttagaatca caaaaaaaag aggtggggag acggtgggta atttcaaaaa
     6121 gtggggagca aaagtgggga gtagtgggga atgaacagaa aaaccagaga taagacagcg
     6181 ccaaaatata aagcgttaga catgacagag ctttccctaa aggtggcaat cagaacgata
     6241 gaccgacacg taggggaagg atacgcgaag gaacatcccg acctgataag cgcattcatg
     6301 accacagcag cggcaaactt tgccacgctg acagaacggg agattgccga agcggaacag
     6361 gtaacaacca tcaacgttaa aaccggagag gtggaatcat gagcgaacca aaatttggtg
     6421 aaaaacttta caagcacaac ggacggatca cgattcagca aataagcgca gctacaccag
     6481 gctggtgggt tgaaacagac gaaggatcat caaccgtggc atcatgggcg ctttgtgtgc
     6541 ttagttaccc tgaccgcgat gtgtatcagg atattctgcc tgtgatatcc actgacaaag
     6601 gcatgaaacc cgtagacatc aaaaacatgg gttttcagtg cgtaatgctc actgaaaaaa
     6661 tgatggaaga gatgaaaaag aataacgccg gatcgcttca ctgaggtggc aatatgacag
     6721 cacaaattgc agcttacggg cggctggtgg ctgacccgca gttaaagacc accagcaagg
     6781 gtacacaaat gacgatggcg agtatggcgg ccccccttcc gtgcagccag gcagatgacg
     6841 gaacggcgac gatgtggtta tccgtcctgg catttggcag acaggccgaa tcactggcaa
     6901 ggcaccgcaa gggtgaactc ctgaccgtgg cgggtaacat gcagatcagc cagtggaccg
     6961 gacagaacgg ggaaacgcgg cagggttatc aggttattgc agacagcgta atcagtgccc
     7021 gtgcggcacg tcctggcggg aacagacgca aaaccacagg cacacagggt aatcagccac
     7081 cagcgggaga cgatgacccc tacggtgatg atattccgtt ctgaggggtg gggacgatgg
     7141 tacatgaccg catagcggaa gaactggagg cgaaaggctt ttaccggaga gcgtcggcgc
     7201 gatggggtga agtcatgcaa ctggtggaga cagacaagga acggcatcac atcacgatgc
     7261 gacggctgga atgttccagg aaggcacaga ggccaccgga gccgccggat aattacggag
     7321 acctgaaaaa ggcggtcgat cgcacttatg ccgaaatggg tatggatggt gctggtgatg
     7381 aaatatggcg aaattaccag gacagataat cacacagccg gagcaatccg gctttttgtc
     7441 atgttttgta aattatttgt tcgtggttgt tccacgttgt tcgctgaccg gatcggcata
     7501 ttttacccga cctgaatcat gattattctc gcccgtggtg ccaggacgct ggggccattt
     7561 tcccgcctgt taatgtgctc gccaatattc attaccaggc gggaaaacga tcggtgcgat
     7621 tactgatttc cttatgaaaa acggttgagt gtttgccgcg tcctggagtt ccttacttaa
     7681 ccccaggact tttttttatg ccgagaataa tcgaattacg ccagcagaaa accgccatta
     7741 aaaatcagat gcgcgacatg ctggagaacg cggaaaaaga aaaccgcagc cttaacgatg
     7801 cagagggcgc aaaatttgac gaattacgcg ctaaagctga atccctcgat aaagacattt
     7861 cccgccttga agccattgca gacgaagagc gcagcaagcc aggtaaaagc agtcagacca
     7921 ctgaccccgc agaactccgc aactacattc tgacaggtga aacccgcgca ttaagtacag
     7981 gcgttcccgc tgatggtggt tataccgtta tccctgaact gaacaccgaa atcatgcgaa
     8041 tgctggcgga tgaatccacc atgcgccgca tctgtaccgt gaagaaaatc agcagcaacg
     8101 agtttaagca gcttgtttcc gctggcggtg cgaccgttaa ccacggtgaa gagggtaagg
     8161 cacgcgaaca gaccagcacc ccgcagatta acgaggtgag catcaagctg tatccggtct
     8221 atgcgtaccc gcgcaccaca caggaaatcg tggatttttc cgatgtggac atcctttcat
     8281 ggctgacggg tgagattggc gacaccttca cggaaaccga agaaagcgat ctggttgtgg
     8341 gcgacggtga caaaaaagca aaaggttttt tatccgtacc ccgtgcagag aagaacgaca
     8401 aagagcgtga ttttggtacg ttgcaggtaa ttaaaccttc cgaatctctg gcgtggacat
     8461 ctgcggaccc gctgatcgac ctgaaatttg cattacgtaa aaaataccgc aaaaacgcgg
     8521 tctgggtggt taactccacg acggcggcaa aacttcagaa ggtgaagaac gcgaacggtg
     8581 attatatctg gcgcgaccgt ttacaggcgg gtgatcctga tacgttgctg ggccttccgg
     8641 tcgaatatct ggagtttatg cctgataacg ttattgccct gggtgacttc aaacgcggtt
     8701 actacattgt tgatcacgaa acagatgttc gcaccagacc ggacaacctg acagaaccag
     8761 gattcatcaa aattttcacg caaaaatatt taggcggtgg cgtggtggat tcgaacgcga
     8821 tcaagattct ggaactgccg gaagacgacg attaacagca tacagaaggg gcttaaaagc
     8881 ccctttagtg ttttatgggt gaaaaaatta tgaagagtat ggaaatccgg tcatcggaaa
     8941 tcaccaccag cgccagcaac acgcttacag gctacgttgt tcgctgggat aacctttcag
     9001 agctgctatg gggggagttt tacgaaaaat tccagcgggg agcgtttact gaatggcttg
     9061 cggcgggtaa tgacgttcgc ggcctgtatg agcatgacca cagcatgtta ctggggcgca
     9121 cccgttccgg cacgctgaaa ctggaagagg acgaaacagg gttacgcttt gaactgaccc
     9181 caccggatac cagtacaggg cgcgacgtta tcgaactggt taagcgtggg gacatctcgg
     9241 ggatgagctt tggctttcgc tcccgtaagg atgtatggga taccacaaca gatccatgcg
     9301 tgcgcaccgt gctggtggcg gaactgtacg aaattaccgt tacatcggta ccggcttacc
     9361 ccgattccgg cgtggagctg gcccgccgtt ccctgtatga gcagcacccc gaaaaaatgc
     9421 cgcgtgcgga taatcgccgc tggtgggcgg atttagcggg ggtgtgatat gtggcctttc
     9481 agaagaaaaa aagagcagcg cagcatgacg cttgatgagt ttatggcgct ggctggcaca
     9541 tcgaacacgg gggcgggtga gtacgtatca tcggggacag cggaatcact gcccgccgtc
     9601 atgaacgccg tcacggtcat ctctgaggcg gtggctacca tgccgtgtta cctgtacctg
     9661 gtacgcaatg agaaggggaa ggaggcccga gagtggcttg attctcatcc ggtcgatcac
     9721 atcctcaacg agcgcccgaa cgcgtggcag actccctacc agtttaagcg aatgatgatc
     9781 cgccactgcc tgttaaacgg taatgcttat gcggtgattc agtgggggcg tgatggtttt
     9841 ccggtggctt tacatcctta cccgccgcag tcggtgaacg tggagcagac aggcgaacac
     9901 aactggcgct actgcatcac tgacgcctac accggaaaca cccgcaacta tttaccgtgg
     9961 gaggttctcc accttcgtta ctccacggat gacggtttta tggggcgctc acctgtaacc
    10021 atctgccgcg aatcgctggg gcttgggctg gcccaacaac gccacggcgc gagcgtgatg
    10081 cgtgatggca tgatggcggc aggggttatc acgtcaggcg aatggctgga cggcgtgaag
    10141 ggcaaacagg cattagccgc actggaacgc tacaaagggg ccagaaacgc cggaaaaacg
    10201 cccatccttg aagggggtat gagctatcag cagctgggca tgagtaatca ggatgctgaa
    10261 tggctggcct cccgtcgctt caccattgaa gacatcgccc gaatgttcaa cgtctcgccg
    10321 atttttttgc aggaatacag caacagcacc tacagcaatt tcagcgaggc aagccgcgca
    10381 tttctcacca tgacgatgcg cccgtggctg gcgaactttg agcagcagat aaaaaacgcc
    10441 ctgctggtgg cctcgcctgt acctggtatc cggtatcagg tggagtttga cagcgcggac
    10501 ctgttacggg ccacaccagg cgaacgcttt gccacctatg aacgcggcat caaatccggc
    10561 gttatgtgcc cgaacgaagc ccgcgaacgt gaagggctgt ccccgcgtga tggtggtgat
    10621 gagttcagcc aggcatggaa acaggaagta aaaatcagcg agggagaaaa accggaatga
    10681 acatagggcg actgcgtgac agggtaacga ttcagaccct gaaacagacc agggatataa
    10741 ccggcgaaat actcgaaacg tgggaggacg gtcacacact ctgggcaagc gtgaacatga
    10801 tcagcagcaa ggaggccatt tcatcgggtg cagagctggc gattggtacc gtaaggatct
    10861 ggatacggta ccggaaggac atcaacgcca ccagccggat aaaggtcagt acggggccgc
    10921 tggcggggcg tgtactgaat atcatcgggc agccgctgcc ggatgccgcc aggacacgcc
    10981 ttgaaattct ttgtcgtgag ggcgcggaaa aatgacagaa gaacttatca ccctggaaga
    11041 agtgaaactc cattgccgca tcgatggcga cgaggaagac cagttaatca gcggatacat
    11101 tgccgcatcg cttgaggcgt gccagataca tataggcagg cgctttgatg acgggctgga
    11161 gttcacgcca gccataaaga ttggctgcat gatgtttatc gctcacctgt acgagaatcg
    11221 ccagctggta gcggataacg caaaaacgcg cgtacccatg acgattggcg cgctctggac
    11281 ggcttaccgt gatgtggggg tgtactgatg ccgtggcaac cattaagacg atgcacagaa
    11341 ccaggctgta acaggcgcgt gaagtccggc aagtgtgagg agcacaggcg ggcggcatgg
    11401 cgtgcagagg atgccagacg aggacaccgc cgcgcgcgtg ggtactccag acagtgggac
    11461 aaataccgcg ccatgtatct gagtaaaaac ccgttatgcg tgcgatgcct tgagaagggg
    11521 atatatacgc ccgccgtggt ggtggatcac attatcccga ttaatggcgg tgatgatgtt
    11581 ctcttctggc ccgaatggaa ccatcaacca ttgtgccagg cgtgtcataa ccagaaaacg
    11641 aaatggcttg atccggcaac aaaaagcaag cgtgccgcag gtggatttca tgaagaggaa
    11701 gaacgggccg ctaaccgcaa taactggatg tatgacgctg atgaatgagc gggaacaaaa
    11761 ccgccttatc cgtggactga taaggcagcg tgacgcatgg aagacacagg aaacagggca
    11821 taaagataaa gcgtcaggac gcgcagaacg catcacagcg aagcgattaa ccgaccgtgg
    11881 ccgcgaggtt atggaatgtt tccgcaatcg ctgatgaggc tgtatgacgg ggtggggggc
    11941 gttttcagga caaacccgac cccgccgggc accgaacgcc tcctcaaatt tttatgcacg
    12001 ggaatttttt gaaaaataat ctgacgaaaa ataagcatgg caagaccacc gaaagccccc
    12061 gcctacctgg atgacatcgc cgtgaagcag tggcgggaaa aatcgcggca gcttgcggaa
    12121 cggggagacc tgacccccgc cgactggagc aatctggaac tgtattgtgt caactactcc
    12181 atttaccgga aagccgtcgc agaccttgcg gcgcgcgggt tcagcattgt taacagtcag
    12241 ggcggcgaga gcagaaaccc cgcattaagc gcgaaatcag acgctgaaag ggtgatgata
    12301 aaaatggcct ccttgctcgg ttttgacccg ataagccgcc gtaaaaaccc accggaaaca
    12361 gaagaagagg acgagcttga ccgcctggaa taagtacgca gaagacgtaa aaacgggcaa
    12421 aattccggcc tgtaaacggc tgaaacaggc cgttaaacgg tacttttcgg accttaaaag
    12481 ccccctttac acgttcgatc gtgaggttgt ggagcggttt attgcctttt ccagggtgtg
    12541 cccgcacgta aaaggaccga tgcgtggcag acccattgag ctggagccgt ggcagcagtt
    12601 cgcctttgcg tgcatcctcg gctttaaggt taaggccacc ggacggcgca aatacacgag
    12661 cgcctttatc gaagtgccgc gcaaaaatgc gaaatccacg accgccgcga ttctggctaa
    12721 ctggtttctg attatggaga acggtcagca ggatatctac accgcagcgg tgagccgtga
    12781 ccaggcgcgg atcgtctttg atgatgcgcg tcagatgtgc cttttatccc gaccgttacg
    12841 caggcgggtg aatattcagg cgcataaggt gatacacccg aaaagcaaca gcctgttaaa
    12901 gccgctggca gcaaaagcgg caaccatcga gggaacaaac ccaagccttt ccatcgtgga
    12961 cgaataccac ttgcaccccg ataacggcgt ttattccgcg cttgagctgg ggatgggcgc
    13021 acgtcccgaa gggctgttat ttgccattac cacatcaggc agcaacgtcg tttcagcctg
    13081 taaacagcac tatgattatt gctgccagat cctggacggc gaagaggtta acgattcaat
    13141 ttttgtactg atttacgaac tggacgacga aaacgaggtt gatgatccgg cgatgtggat
    13201 aaaggcgaac cctaatattg atgtttccgt cgatcgtgaa aaactggcct caaccatcca
    13261 gaaagcgcgg ggtattccgt cgcagtgggt ggagatgatg acaaagcgat ttaatatctg
    13321 gtgccagggg gctaccccgt ggatgggtaa cggcgcatgg gctgagtgtg ccggaacgtt
    13381 cacggaggaa gatttacacg gccaggagtg ttacgcgggg ctggacttat catcaaccag
    13441 cgatatttcc agcgtgtgct atgcctttcc ggtcggtaaa accattatgc tggtttcccg
    13501 tcactatctg ccggaatttc agctacagaa ccccgccaat aaaaaccgcg ccatctatcg
    13561 tcagtgggct aaagcaggct ggatacgcac aacgcctggc gactgcattg attacgaccg
    13621 gatcagagat gacatcatgc aggacgcgga gaaatttaat atcaggctgg tgggctttga
    13681 tacgtggaac gccacgcatc tcagaacgca attacagggg gcaggttttg aggtggagcc
    13741 gttcccgcaa acctacctca gattcagtcc ggcggcgaaa tcgttcgaag tttttgttaa
    13801 ccgcagggtg attgtgcatc gtggcgatcc ggtgttgtcc tggtcgatga gtaacgtcgt
    13861 gatgcagagt gacgcgaacg ccaatatcaa gccgaacaag aaaaaatcac cgaacaagat
    13921 agacccgagc gtggcggcgc tgatggcgtt tggtacattc caggcagagc atgaggattt
    13981 tgcttttgat ataagcgaca gccaccgcca gaaactggaa gaatttagcg gggtgtaatg
    14041 aggtcagcag catgacagaa gccgaaataa cgggattaat ccgccgcgtt gccggaatca
    14101 gccagcaggc tgacgaacag gccacgcagc cggacagcgt gacagccgaa aactatgcgc
    14161 gtgttgttgc tgaggtgatg cgccgtgatg gtatagaact taatggcgtg gatatgcgca
    14221 acatacgaac cagagttctt gagttgctgg cctaccgtcg ccgcgtggag atgtatcggg
    14281 agaaggagaa aataacgtac cactggaaga agccggagcg gttgcggcgg taactggttg
    14341 atattcccga cggcccaaaa atgggttggc tacacccctc acaactacga cattttcgca
    14401 gtttaattaa tggtcagctt aatcgctaac ccgctgatat tttcggaaac ctcaatttga
    14461 ggaagtcggc gcggtaactc gctgaacttt aagcaaagcg caaaattgcg cttgctgaat
    14521 aatcattatg attacgcaga tgattaagga atgaccgaag gcggaaattc gcctgtggtt
    14581 aatgggggag ttgcagatct gcaactcgac catgaaacta cggaaactac ccgtagtttg
    14641 ggtagtaaga gtaacaccca gattttgggg cttactcgtg atacccaaat ttggggtatc
    14701 agtggcaacc ataacgactt tcgttacggt tgatgctttt accccattgg gggaaaggta
    14761 ttacgataat cataacacct accgaacagg taaagcccac cagcctgatt aacgtttaac
    14821 cggaaaaaaa ttccggtagg tggggatccc catatcgaca ttaacgcccc tcatgaattg
    14881 gtgtgcttcc ccctggaaag atgatccgcc tgtatatttc ttgtgtctat ttgttccacg
    14941 ttgtttcata cagtgcaccg aacggtgtag tcactggtgt agtcattttg cgatttttgg
    15001 cactttttgc ggtgttaatt atttaattaa atcaatgtgt tatgcgaaat aagttatata
    15061 taacagcaat aaataatgcg agaataaacg gcacgatgat ctcggcggca aaacggatac
    15121 cgcagagaat aatgaccagc attcccaaca taatgacgat ttttaggcca ttgagcgtga
    15181 tgatcggctt tgccatgctt gtccccgttt tttcttcctt tgctaataat aatgtctttt
    15241 tcgctccgtc atcataacga aaatcaaacc gctcaggtaa agaagtgaaa atattttgag
    15301 ttaattctta agctatgata caaatcaggc gtgttcaact accgaggaca attatcatct
    15361 gcgatgacga gaagcaacac tgcggataat tgtaatatta tggacaattt gttcaggacg
    15421 ttattttcta catttaccca tcttcgtacc tcatccacta tcttgctcgt aggtgagcag
    15481 cactggcgca acgcgcttta gtcctttctt ctgcctaagc ttgccctatg cgcagggctt
    15541 gaccacttta ctttggtttc gctgaattaa gcgaaaatta aaatagttct cttgcaggag
    15601 aaggacaatg tatatttatt ggattttatt aggtctggct attgctacag aaattaccgg
    15661 tacgctgtca atgaaatggg cgagcgtcag tgagggaaat ggcggcttta ttttaatgct
    15721 ggtgatgatt tctctgtcgt atatatttct ctctttcgcc gttaaaaaaa tcgccttagg
    15781 cgtagcttat gcgctgtggg aaggtatcgg tattttattt attaccttgt ttagcgtttt
    15841 gttattcgac gaaagtttat cgctgatgaa aattgccggg ttaaccaccc tggtcgccgg
    15901 gatagtgttg atcaaatcag gtacccgtaa agcgcgtaaa cctgaactgg aggtgaacca
    15961 tggcgcagtt tgaatgggtt cacgccgcct ggctggcatt ggcaatcgtg ctggaaatcg
    16021 ttgctaacgt ctttttgaaa ttttctgacg gctttcgtcg caaaatattt ggcttgctct
    16081 ccctggcggc ggtgctggcg gcctttagtg cgctttctca ggccgttaaa gggatcgact
    16141 tgtctgtcgc ttatgcattg tggggcgggt ttggtattgc cgccacgtta gccgcaggtt
    16201 ggatcttgtt tggtcaacgg ttaaatcgta aaggctggat tggcctggtc ttgctgttgg
    16261 ctggaatgat catggtgaaa cttgcctgat gaagacgctg cccgcgctgt cgggcagcgt
    16321 ttgaacatta tttttgcgac agttgatcca gcttgtcgcg aaaaccggta acagaaatgg
    16381 cccggttatc ggcgcgccag cgatctttcg cggcaggagc cgaactttgc accccaatta
    16441 attgccagcc gtcatcggta tgcaacatca gaggcgaacc gctgtcaccc ggcaaggtat
    16501 cgcactgatg tgacatcacc gacgtttgcg cccagccagt cacttcacag ttttgatgac
    16561 tgtataacgt atcgagatga tcttcagggt atcccgcctg agtcacttta cgaccagccg
    16621 attttaatgc ggcagtaagc gcggctttat ctccctcaaa taacggcaac ggtgtaatgc
    16681 cagaaggggg attacgtagc acaatcaatc cgaagtccca cggtgcagct gcgggaggaa
    16741 caatccaacc atccccatct gcttttaacc gctttcccag tgtcggatca acgcggcctt
    16801 ctatgtcgtg gatctcatag cgccaaagac ctttatttga cacaaaacgc agcgctaccg
    16861 ccttatccgc tttaccctta ggcggcgtca ataaacagtg tcctgccgtt aatgccagat
    16921 tgggtgcaat cagcgtcgcc gtacataaat tgccgctggc cgtttccagt tgcccaaccg
    16981 catcccacgg tgattgggtc gtgtcattca ctggcacacg atcatcatga ccaaaaaaca
    17041 gggtgctgac ctcatcgttt gccgatttgg caacgtctgg tttatctgca aacacaaaag
    17101 cagacgtcaa actaattgca cccaacacta cagcaatggt tgtacgcata tcacactctg
    17161 gtgggtaatt atgattatta aaagtaaacc ctcataaata ctatagacgg gacgggatga
    17221 aagtgggagt aaaatcagat aactacagtc aggaaagata aaaacgggcg gcaatcagtg
    17281 cgcataaaat aaggatgata agaatgaact caaagcgata acggcccacc atactaccct
    17341 ccgctaaagg cggtgctaaa cgttacccgc ttagcaccgt aattttcagg cgcgaggggg
    17401 cgcgccagca ttactgttga aaacttacgc tgcgggttgt gcagcaggtt ttgccggttg
    17461 ctgatggccg tgtttcccgg cgtgtttctt cgctgcctgc gctttttgtt caggggcttt
    17521 ctgttcagct ttcgtatttt tatgatgctt tttagccgcc tgcgctttct gggcaggtgc
    17581 tgctttatgc tgttttttat gatgtgtagt tttcgctggc gctgctttgg tggtcgtcgc
    17641 agtcggagca ggtgtggtcg cagtctctgc agcaaaggcg gcagaagaca gacccatagc
    17701 agcggcaaca accagagcta atactttttt cattgtcata ccctcaattt gttttttcat
    17761 ttaaccccac tgcggggccg ttgaaataac tatatccctg ggaatggcag acttccgtga
    17821 gtggttggtt tcagcgtgta acgatatgta caaacgctga ataaattaca gcgttgataa
    17881 taacgtattg tgacttaagg aaaatttagc taccaataat agtagtcttg atcgggttaa
    17941 ctattatact acttaca
//
LOCUS       DBKEKI010000070        17827 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.070, whole genome shotgun sequence.
ACCESSION   DBKEKI010000070 DBKEKI010000000
VERSION     DBKEKI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.070"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..665)
                     /gene="clsB"
                     /locus_tag="ACUQPX_002471"
     CDS             complement(<1..665)
                     /gene="clsB"
                     /locus_tag="ACUQPX_002471"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HHV9057160.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARR"
     gene            complement(662..1423)
                     /locus_tag="ACUQPX_002472"
     CDS             complement(662..1423)
                     /locus_tag="ACUQPX_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HHV9057161.1"
                     /translation="MPNQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            1556..1966
                     /locus_tag="ACUQPX_002473"
     CDS             1556..1966
                     /locus_tag="ACUQPX_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HHV9057162.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(1928..3034)
                     /locus_tag="ACUQPX_002474"
     CDS             complement(1928..3034)
                     /locus_tag="ACUQPX_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9057163.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(3045..4178)
                     /locus_tag="ACUQPX_002475"
     CDS             complement(3045..4178)
                     /locus_tag="ACUQPX_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9057164.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGMPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(4171..5907)
                     /locus_tag="ACUQPX_002476"
     CDS             complement(4171..5907)
                     /locus_tag="ACUQPX_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHV9057165.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(5900..6895)
                     /gene="hlyD"
                     /locus_tag="ACUQPX_002477"
     CDS             complement(5900..6895)
                     /gene="hlyD"
                     /locus_tag="ACUQPX_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HHV9057166.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPMTVQFGDEAGHE"
     gene            complement(6898..7569)
                     /gene="cecR"
                     /locus_tag="ACUQPX_002478"
     CDS             complement(6898..7569)
                     /gene="cecR"
                     /locus_tag="ACUQPX_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HHV9057167.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            7798..9162
                     /gene="rhlE"
                     /locus_tag="ACUQPX_002479"
     CDS             7798..9162
                     /gene="rhlE"
                     /locus_tag="ACUQPX_002479"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HHV9057168.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(9394..9876)
                     /gene="ybiA"
                     /locus_tag="ACUQPX_002480"
                     /pseudo
     CDS             complement(9394..9876)
                     /gene="ybiA"
                     /locus_tag="ACUQPX_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
     gene            9996..12146
                     /gene="dinG"
                     /locus_tag="ACUQPX_002481"
     CDS             9996..12146
                     /gene="dinG"
                     /locus_tag="ACUQPX_002481"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HHV9057169.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIAKKKEKTKSPRRRRR"
     gene            12174..13136
                     /gene="ybiB"
                     /locus_tag="ACUQPX_002482"
     CDS             12174..13136
                     /gene="ybiB"
                     /locus_tag="ACUQPX_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HHV9057170.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVVKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            13277..14362
                     /gene="hcxB"
                     /locus_tag="ACUQPX_002483"
     CDS             13277..14362
                     /gene="hcxB"
                     /locus_tag="ACUQPX_002483"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HHV9057171.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            14429..14488
                     /gene="ybiE"
                     /locus_tag="ACUQPX_002484"
     CDS             14429..14488
                     /gene="ybiE"
                     /locus_tag="ACUQPX_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HHV9057172.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(14591..14851)
                     /gene="ybiJ"
                     /locus_tag="ACUQPX_002485"
     CDS             complement(14591..14851)
                     /gene="ybiJ"
                     /locus_tag="ACUQPX_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HHV9057173.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(15116..15382)
                     /gene="ybiI"
                     /locus_tag="ACUQPX_002486"
     CDS             complement(15116..15382)
                     /gene="ybiI"
                     /locus_tag="ACUQPX_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HHV9057174.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(15456..16133)
                     /gene="ybiX"
                     /locus_tag="ACUQPX_002487"
     CDS             complement(15456..16133)
                     /gene="ybiX"
                     /locus_tag="ACUQPX_002487"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HHV9057175.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(16175..17827)
                     /locus_tag="ACUQPX_002488"
                     /pseudo
     CDS             complement(16175..17827)
                     /locus_tag="ACUQPX_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4085 a         4695 c         4859 g         4188 t
ORIGIN      
        1 tcccgccgcg cctgagtgag cattttcaga taatggcgtt caatatcatc gcgatgttct
       61 tcgttatcgc gccagaccag caatacctgc gcttctcccg gctggcggtt ctcttccgct
      121 ttgtgatgac gtcgccacca gcgtcgtgcc gcgctctgtc caggcaggtt ttccagctca
      181 aactggagaa tatcttcgac aatcggccct tcaaggcgta ccgcgtaatc ctgtttagcc
      241 tctggaccgt agctggacat atgctcggcg gagtaattca gcccgccaat aaaggcgata
      301 cgcgcgtcga tcaccacaat tttgcgatgc atccggcgaa acacattggt gcgcatacca
      361 aaaaggcgag ggcggggatc gtagtagcgg aacactacgc cagctgccgt cagttcattg
      421 acaaactcat cgctgagatc cggcgaaccg tagccatcca gcaagacttc cgctttaacc
      481 ccgcgttgcg ctgctgccag tagtgccgca tgcagctgtt tgccgacgtt atcctcaaac
      541 cagataaacg tttcaagaat gatgcgttct tgtgcctcgc caatcgcctt aaacaccgcg
      601 ggataatatt gctcgccgtt ttccagcaac tggatcttat tgccttcgcg ccagctacat
      661 ttcataaatg aatctccgca cttaaagggg catgatcaga aaggtgtcgc catgtccgca
      721 gcggcaacgc ggttggcgcg ctggcgctgg catttttgac gtagatcctg tccagtcgta
      781 gtagaggaaa ttgcaccgga aacgtgcgcg ccgggcgtcc gtgggcgcgg gtaaaaatct
      841 catccagtcc ggcctgcact tttaacggat gattagcttt ttgccgccag tcattgaaat
      901 cacccgccac caataccggt tcgccgtccg gtagctcatt cacccattcg gcgagcatcg
      961 caagctgcgc ctgacggtgc gcctcacgca ggcccagatg tacgcacatc acatgaatcg
     1021 cttttccggt catcggcggc acaatgcggc agtagagcac gccgcgcttt tccgcaccat
     1081 cgaccgaaac atcgcgattc tcataatgtt caatgggata acgcgacagt acggcgttgc
     1141 cgtgatgccc ttccgggtat acggcattgc gaccgtaggc aaaatcgctc cacatagtgt
     1201 cggcgagaaa ctcgtagtgc gaggtatcgg gccagttttc cacatgcagc ggatgaactt
     1261 cgtgcgcgcc catcacttcc tgcaggcaaa caatatcggc gctgacggta cgcacggcat
     1321 cgcgaagttc cggcaaaatg aagcgtcggt taaacgcggt aaagcctttg tgaatgttga
     1381 tggtgagcac cttgaacgaa aattgttgtg tttgattggg cataattttc ctgtctttgc
     1441 ctcttatctc attgaaatag tgtagtcggc gtcacaaaaa ggtgcggtct tacggaattt
     1501 tccgtaaagt tcggtactct gagtaagtag agataaattc ttcaggagag aagccatgaa
     1561 gtggcaacaa cgtgttcgtg tcgcaacggg tctaagttgc tggcagatta tgttgcattt
     1621 actggtagtg gcgctgctgg tggtgggctg gatgagtaag actctggttc acgtcggcgt
     1681 gggattatgc gcactgtatt gtgtcacggt agtgatgatg ctggtgtttc agcgccaccc
     1741 cgagcaacgc tggcgtgagg tggcagacgt gctggaagag ctgaccacga cctggtattt
     1801 tggcgcagcg ctgattgtgc tatggctgtt gtcccgcgta ctggaaaaca actttttgct
     1861 ggcaattgca gggctggcga tccttgctgg cccggcggta gtgtctttgc tggcgaaaga
     1921 taagaagtta catcacctta cgtctaaaca tcgcgtacgc cgctgaccct gtcgtggccg
     1981 ttatcaccag tagcggccac aaactattcc acacaatatc cagactcgca tccttcaaat
     2041 aaatctgctt ggtaatgtcc gtaaagtggc gaatagggtt aatccacgtc agattttgca
     2101 gccacactgg catgttttcc accggcgaaa cgtaaccgga aaggagaatg gcgggcatca
     2161 taaaaacaaa cacgccgata aacgcctgct gttgtgtcga gcagagtgat gaaatcaaca
     2221 gaccgaatcc caccagcgat aaaccgtaaa tcaccatcgt aaagtagaac agcgctagcg
     2281 atccggcgaa ggggatttga tacgcccaga taccaatcgc cagcacaatg gtggcctgga
     2341 aggtggcgac aattaacgcc ggtacggctt tgccgatgaa gatctgccag gtggtgagcg
     2401 gcgaaaccag tagctgatcg agcgtacctt gttcacgttc gcgggcgacg gaaagtgaag
     2461 tgacgatcat tacgccgata gtggtgatca tggcgatcag cgacggcacc acaaaccatt
     2521 tgtagtcgag attcgggtta taccagttgc gtaccaccag ctcgctgttg ttgggtttcg
     2581 gttttccttc cagcagctcc tgctgataat ttttgacgat ctgttgcagg tagttggcgg
     2641 caatttgcgc actgttggag ttacgcccgt cgaggatcaa ctgcaacggc gcggtctgga
     2701 aggtatccag tttgcgcgag aagtcagccg ggaaacgcac cagtagtaac gccttttgtg
     2761 tgtcgatggt cgggcggatc tcctgtgggc ttttcagcag cagcacatgt gtaaaggcgc
     2821 tggcgcgggc aaaacgttga gtaagttcca ccgaatgctc gccgttatct tcatcgtaga
     2881 tggcgatggt ggcgttagtc acttccagcg tggcggcgaa cgggaacagg atcacctgaa
     2941 ttagcacggg taaaatcaga atcgcgcggg tttgtggttc gcgcagcaac gactgcaact
     3001 ctttgcggat taacgtccat aagcgatgaa acatgctctt ctccctaatc cagccgacgt
     3061 ttggttttca gccacgtcag gccgataaac atcaccgccg aagcgatcaa aaacagcacg
     3121 tttaccacca gcaccactgg aatattcccg gcgaggaaca ggctttgcag ggtgctgacg
     3181 aaataacgag cgggaataat gtacgtcacc gcgcggatca ccgcgggcat actgtcgatc
     3241 tgaaaaataa agccggaaag cataatcgac ggcagaaaag cggcgttcag ggcgacctga
     3301 gcggcattga actggttgcg ggtaatcgtg gaaatcagca gccccattcc cagggtactg
     3361 agtaaaaaca ggctggagat aaaaaacaga atcagcagcg aaccgcgata cggcatgccg
     3421 agaataaaca ctgacaccag catacacagc aacatcgcca gcatcccgag aaagtaataa
     3481 gggatcagct tacacagcag cagttccgtg cgggtaatct ccgtagagag cagagcctcc
     3541 atggtgccgc gttcccattc tcgcgccacc accagcgagg tgagaatcgc gccgatgacc
     3601 gtcatgataa tggtcaccgc acccgggata ataaagtgct ggcttatcgc cgccgggtta
     3661 aaccagtagc gggtttgtac atcaataagc ggttcaaaag tctgcccgtt gtcctccgct
     3721 cgctgcattt gccagatctg ccagatccct tcgacatacc cctgcacaaa gttagcggta
     3781 ttcggctcac tgccgtcggt gatcacctga atcggtgcgg tggcgttggc gcgctccatc
     3841 tgttcggcga aatccacggg aataaccacc agaccgcgaa ttttccccga ctgcattttg
     3901 gcgatcagtt cctgacggtt atcgctgatg gtggcgtcga tgtagggcga accggtcatg
     3961 gtgtgggtga aatccagcgc cgcttcgcta cgctgttcca gcaaaatccc gacccgcagc
     4021 ttgctggagt cgaggttaat gccgtaacca aaaataaaca gcagtagcag tgggatcact
     4081 accgcaatca gccagctact cggatcgcga acgatctgcc gcgtctcttt gacgcacagc
     4141 gcccgcacgc gacgccagga aaggatcggg ttactcattg ctatgctcct tatcccagtc
     4201 gtggatcaac tgaataaagg cttgctccat ggtgggatcg ggttgctcat cgttagctga
     4261 ctgtgctttc aaatcgtccg gcgtaccgct ggcgattaat ttcccacggt acaccaggcc
     4321 gatgcggtcg caatattccg cttcatccat aaagtgggtg gtgaccatca ccgtgacgcc
     4381 tttctctacc atgctgttga tgtgcagcca aaattcacgg cgggtgaggg ggtcaacgcc
     4441 ggaagtcggt tcgtcgagaa agagaatatc cggttcatgc atcagcgaac aggccagcgc
     4501 cagccgctgt ttaaaaccta atggcagttc atcggtggcg tgtgaggcaa tacttttcag
     4561 gccaaatgcc tcgctcatac gggagatttt ttcgttctgc gcccgaccgc gtaagccata
     4621 cacaccagag aaaaagcgta aattctgttc gaccgtcagg ttgccgtaga gcgaaaattt
     4681 ttgcgccata tagccgagat gctggcgcgc tttaccggaa ctctctttca gatccatccc
     4741 cagcaccagc gcctggccgg aagtcggcac cagcaaaccg cacatcatct taaaggtggt
     4801 cgatttaccc gcgccgtttg gccccagcaa accaaaaatc tccccacgtt taacggcaaa
     4861 gttgacgtga tcggtggcgg caaaatcacc aaatttcttg gtcagttctt tcgcttcgat
     4921 caccgtctcg ccgggtgtgc cttctaccgt atgcaatatt gcacccagcg gcgattctga
     4981 ggttccggca ccgcccagca aatcaataaa cgcatcttca aaacgcggcg tagtttcgtt
     5041 gatgttgatt tccggcatcc cgtcggcatg gcgaatatcg tctggtgtgg cctctttttt
     5101 gaggatcaga cgtaccgatt tcccctgaat catgccgtcg ctgacctgcg gcagtttcaa
     5161 ggcgcgttgc aacagtttgc ggttgccctc gtgtggactg gtcatcagaa agctgcgtcc
     5221 ggccatggtt tgtgtcaggg cttttggttc tccctgatac agcaactcgc cttcgttcat
     5281 cagtaacacg tcacggcact gctcggcttc gtcgagatac gaggtactcc agaggattaa
     5341 catcccttcg cccgccagct catgcaccat ctgccacagt tcgcgccgtg agatagggtc
     5401 aacgccgacg ccgggttcat cgagcagcaa cactttcggt tcgcccacca gggtacaggc
     5461 caggccgagt ttttgtttca tcccaccgga gagcttgccc gccaggcgtc cggtaaacgg
     5521 cccaagagac gtaaactcca gcaggcgagc aaaagtttgc ttacgtgcct cgccggtgac
     5581 gctgcgcaaa tccgcgtaca gattgaggtt ctccatcacc gtgagatctt catacagacc
     5641 aaatttctgc ggcatatagc cgagcacggc gtgcagcgcg ccgtcgtttt tgatcggatc
     5701 aaagccaatc accgtggcac tgccgctgtc gggtttcagt aatcccgcca acatccgcat
     5761 cagcgtggtt ttacctgcac cgtccggccc caccaacccc gtcacataac cggcgtgaat
     5821 ggtacaatcg agcggcgcga cggcgggctt gtccatgccc ggaaagcgtt tttccaggcc
     5881 gttcagcgtg ataacggcat cattcatgtc ctgcctcgtc accgaattgt accgtcattg
     5941 gcattccctg gcgtaacgca tcatcggcgt cggtcaccac aatacgcagg cgatagacga
     6001 ggtcggtacg cagatccggt gtttcgacgg tttttggggt aaattcagca gtcggcgaaa
     6061 cgaaaccaat ctgcccgtga tacggcttgt ccgggcgacc atcggtataa agcagcactt
     6121 tgcgccccgg ctgggcctgg tcaagattcc gttcatcaac ataagcgcgc acccacaccg
     6181 gacgcgttag tgaaacggta aacaccgtgc caccttcatt gagaaccgtg cctggctcta
     6241 ccgcgcgcgt taacagcgtg ccatcagacg gtgctatcaa cgttgagtcc tgtaaattca
     6301 gctccgcctg cgccagttgc gcctgcgcct gttcgaggct ggctttcgcc tgagcgatgt
     6361 cctgttcacg gttaccggaa cggtactgac gcaatttatc ctgtgctgat ttcagcgttg
     6421 cctgtgcctg gtcgcgcgag gagcgggcat tttccaggtc atttgccgaa atggtgcggc
     6481 ttttccacag tccttgctgg cggttataga agttctgcgc atagtcatag gcggcttgcg
     6541 cctgtttcac cgctgcggcg gcctgagcga tttcttcatc gcgatacccg gcaagcatca
     6601 gatcatactg cgcctgtgcc accgagacgc ccgctttcgc ctgcatcagg gcaatctcat
     6661 acggcttgtg atccagttcg cccagcacct ggcccgcttt gatagcatca ccttcgtcca
     6721 ccgccagcga ttcaacgcgc cccccaacac ggaaactaag attcaccgta cgaatatcca
     6781 cgttgccata cagcgtcagg ccgttatcct ggcggctttg ataccaccag tagcctccgg
     6841 caaccacggc ggcaagtacc actaccgcca gtccgatcac gacaggtttt ttcatcacta
     6901 caaactcctt tgcgataatc cttgcaaaat caggtcgata tgacaggtca ccgtctggtt
     6961 gatcagttcg gtcttttctt catcgaacgc ggtccagccg gtacgtaaca gaattgtttc
     7021 tttgccaaga cggaacgcca gaatctcacc aatcaacgca tgggtatgaa ggatcatgcg
     7081 ggtgtcatta gcgtcgcagc cggtccaggc ggcaatcagg cgtgtcaggt ggctgtgtag
     7141 cggactaatc acctgttcgt gcaccaggtg gtaggctgcc gtgggagaga gctgctcacg
     7201 ggagataaac ttgctgaggt tgacggtgtc atcctgggtg agcagcttaa tcatgttcct
     7261 gcaggcgcga aggatcagtt cacggatggc agcccgatca ggctgtggtt gggcgaacaa
     7321 gcgttcggct tcctcggcat gcggacggaa ctgctcgcca ataaaatcgg caatccactg
     7381 ggcgcaggcg aggtacaaat cttctttcga accgaagtag taggtgatgg cagcgatatt
     7441 ctgcccggcc tgggcggcta tctcgcgggt ggtggcgttc attccatatt caccaaactg
     7501 cgccagtgcg gcagcaatca gctgtttttt cgcctgttca cccttgattg tcatggcagg
     7561 attattcatc gcacagtcca ttcttaatca aatgattgat taagattatg actccatatg
     7621 ggagttgtcc agtatggcta agaattttag ctacgccagt cacagggata atttatgcgc
     7681 tgcgtcacaa aaactgctac actccgctcc ctcatgacat tgtggttttt gtcattttcc
     7741 ttttcagtat ctccctgaaa actacaccgg taacggtcgg ggcggttcgg agtagttatg
     7801 tctttcgatt ctttgggttt aagccctgat atcctgcgcg ccgttgccga gcagggttac
     7861 cgtgaaccca cccctattca gcagcaggcg atccctgcgg tgctggaagg ccgcgacctg
     7921 atggctagcg cccagaccgg caccggcaaa acagcgggct ttacgctgcc gctgttgcaa
     7981 cacctgatca ctcgccagcc gcacgccaaa gggcgtcgtc cggtacgtgc gctcattctt
     8041 accccgaccc gtgaactggc ggcacagatt ggcgaaaacg tccgtgatta cagcaaatac
     8101 ctgaacattc gttcgctggt ggtgtttggc ggtgtcagca ttaacccaca aatgatgaag
     8161 ttgcgcggcg gcgttgatgt gctggtggca acgccgggac gtttgctgga cctggaacat
     8221 caaaatgcag tgaagctgga tcaggttgaa atcctcgtcc tcgatgaagc tgaccgcatg
     8281 ctcgacatgg gctttatcca cgatatccgt cgcgtgttaa caaaactgcc agcgaaacgc
     8341 cagaacctgc tgttctctgc gaccttctct gacgatatta aagccctggc ggaaaaactg
     8401 ttgcacaacc cgctggaaat cgaagtggca cgccgcaata ccgcgtctga tcaggtgacc
     8461 cagcacgttc actttgtcga taagaaacgc aaacgcgaat tgctgtcgca catgattggt
     8521 aaagggaact ggcagcaggt gctggtgttt acccgtacca aacacggcgc taaccatctg
     8581 gctgagcagc tcaataaaga tggcatccgt agtgcggcga tccacgggaa taaatcgcaa
     8641 ggtgcgcgta cccgtgcgct ggctgatttt aaatcgggtg acattcgcgt actggtggcg
     8701 accgatatcg ctgcgcgcgg tctggatatc gaagagttgc cgcacgtcgt caactatgaa
     8761 ctgccaaacg taccggaaga ttatgtccac cgtatcgggc gtaccggtcg tgcggctgct
     8821 accggtgaag cgttgtcgct ggtgtgtgtt gatgaacaca aactgctgcg tgatatcgaa
     8881 aaactgctga aaaaagagat cccgcgcatt gcgattccgg gctatgagcc ggacccgtca
     8941 atcaaagccg aaccgatcca gaacggtcgc cagcaacgtg gcggcggcgg tcgtgggcaa
     9001 ggtggtggtc gcggtcaaca gcaaccacgc cgtggggaag gtggcgcaaa atctgcaagc
     9061 gcgaaacctg cagaaaaacc gtctcgccgc ctcggcgatg ccaaaccggc aggcgaacaa
     9121 caacgtcgcc gccgtccgcg taaacctgcc gctgcgcagt aatcttttat gccgggctat
     9181 gcccggcatc aggctgatga acaaacgcaa aactgcctga tgcgctacgc ttatcaggcc
     9241 tacgtgaact ctgcaatata ttgaatttgc atgcttttgt aggccggata aggcgtttac
     9301 gccgcatccg gcatttcaca acaagcactt gtcagcaatt tgagaacacg ggaaaaaatt
     9361 tatctgtttt accgcccata agccaccgag acgttacttc tctatagcca attgttcgcg
     9421 caactccatt aaaaggtagc ccagtcgatt cttgccctta ccattaccac cgtctcccca
     9481 gtaagcatcg ttttccgtat gctcaaccag ttttgcgggc gcggttgcca gcaagagcac
     9541 acgcagttct gcatgctgtt cgaatttagc gcgaagtgct ttacgcatca cctgttcttt
     9601 aaccgactcc cagtttttac gaagtggctt ggaacgatta cgccccatgc gagcggcgac
     9661 catgggggaa gaaacccgac ggatctcttc gcggtatttt tcatcaagga atttttgtgc
     9721 ctgaaaatag tgttcggagg tgggctaggt attcccgtca accttgatgg gccaggcggc
     9781 aaagttagaa aaatcaccgt agtcatcgct ggtgctgtag aaatttatga tggtgtcttg
     9841 catgacgtgt tggatccttt gtgctcgaac gggcattaaa ccgcattatg ttggtggtta
     9901 ttgcgagccg ctttccagaa acagaaaaac cattacccct gaaaaccgaa aaatgccaca
     9961 atattggctg tttatacagt atttcaggtt ttctcatggc attaaccgcc gcgcttaaag
    10021 cgcaaattgc cgcctggtat aaggcgcttc aggaacagat ccccgacttt attccccgtg
    10081 cgccgcagcg gcagatgatt gcggacgtcg ccaaaacgct ggccggagaa gaagggcggc
    10141 atctggcgat tgaagccccc accggcgttg ggaaaacgct ctcctatttg atccccggca
    10201 tcgccattgc ccgcgaagag caaaaaacgc tggtggtgag taccgccaac gtggcgttgc
    10261 aggatcagat ttacagcaaa gatttaccgc tgctgaaaaa gatcattccc gatcttaaat
    10321 tcactgccgc tttcgggcgt gggcgctacg tttgtccgcg taatctgacg gcgctcgcca
    10381 gcactgaacc cacgcaacag gatctgctgg cgtttcttga cgacgaactg acgccaaata
    10441 atcaggaaga gcaaaaacgt tgtgcgaagc tgaagggcga tctcgacact tataaatggg
    10501 atggtctgcg cgatcatacg gatatcgcta tagatgatga tctctggcgt cgtttgagta
    10561 ccgacaaagc cagctgcctc aaccgtaact gttactacta tcgtgaatgc ccgttttttg
    10621 ttactcgtcg ggagattcag gaagcggaag tggtggtggc aaaccatgcg ctggtgatgg
    10681 cggcgatgga aagcgaagcc gtactgcctg acccgaaaaa tctgctgctg gtgctggacg
    10741 aaggtcatca cctgccagat gtggcccggg atgcgctgga gatgagcgct gaaatcaccg
    10801 cgccctggta tcgactacag ctggacttgt tcacgaaact ggtcgctacc tgcatggagc
    10861 agtttcgccc gaagaccatc ccaccgctgg cgatccctga acgtttgaat gcgcattgtg
    10921 aagagttgta tgagcttatc gcctcgttaa acaacattct caatctctac atgcctgccg
    10981 ggcaggaggc agagcaccgt tttgcgatgg gcgaactgcc tgatgaattg ctggagatct
    11041 gccagcggct ggcaaaactc accgagatgc tgcgtggtct ggcggagtta tttcttaacg
    11101 atttaagtga gaaaaccggc agccacgata ttgtgcgtct gcatcggttg attttacaga
    11161 tgaaccgcgc gctggggatg tttgaggtgc aaagcaaact ctggcggctg gcgtctctgg
    11221 cgcaatcttc cggtgcgccg gtgaccaaat gggcgacgcg ggaagagcgc gaagggcagc
    11281 tacacctctg gtttcactgc gtgggaatac gtgtcagcga tcagctggaa aggctgctgt
    11341 ggcgcagtat tccgcacatt attgtcacct ccgcaacctt gcgttcgctg aatagttttt
    11401 cgcgtttgca ggagatgagt ggtctgaaag agaaagcggg cgaccgtttt gtggcgctgg
    11461 attccccctt taaccactgc gaacagggca aaattgttat tccccggatg cgctttgagc
    11521 cttccatcga caacgaagag caacacattg ccgaaatggc ggcctttttc cgtgaacagg
    11581 tggagagcaa aaaacatctc ggtatgttgg tactgtttgc cagcggacgg gcgatgcagc
    11641 gctttctcga ctatgtgacg gatttacgcc tgatgttgct ggtgcagggc gatcagccgc
    11701 gttatcgctt agttgaactg caccgcaaac gcgttgccaa cggtgagcgt agcgtgctgg
    11761 tgggcttaca gtcatttgcc gaagggcttg atttgaaagg tgatctgctc agccaggtgc
    11821 atatccacaa aatcgctttt ccgcccatcg acagcccggt ggtgatcacc gaaggggaat
    11881 ggctgaaaag cctcaaccgc tatccgtttg aggtgcaaag cctgccgagc gcctcgttta
    11941 acctgattca gcaggttggg cgactgattc gaagccacgg ttgctggggc gaagtggtta
    12001 tctacgacaa acgcttgctg acaaaaaatt atggcaagcg actactggat gcattaccgg
    12061 tatttccgat agagcaaccg gaagtccctg aaggtatagc taaaaagaaa gaaaaaacga
    12121 aatccccacg ccgtcggcgg cgttaatgat gtgagtcagg taaggagtcg taaatggact
    12181 atcgcaaaat cattaaagag atcgggcgcg ggaaaaacca cgcgcgcgat ttagaccggg
    12241 atactgcgcg cggtctgtat gctcatatgc tcaatggtga agtccctgac cttgagttgg
    12301 gcggcgtatt gattgcgctg cgtatcaaag gggaagggga agcagagatg ctcggctttt
    12361 acgaagccat gcaaaatcac accatcaagc tgacgccgcc tgcaggcaag ccgatgccga
    12421 ttgtcatccc cagttacaac ggcgcgcgta aacaggctaa cctgacgcca ttattggcga
    12481 ttctgctgca taaactcggt tttcctgtgg tggttcacgg agttagcgaa gatccaactc
    12541 gcgtgctgac agaaaccatt ttcgaactga tgggtattac accaacgttg cacggcgggc
    12601 aggcgcaggc gaagctcgac gaacatcaac cggtgtttat gcctgtcggc gcgttttgcc
    12661 cgccgctgga aaaacaactg gcgatgcgct ggcggatggg cgtgcgtaac agtgcacata
    12721 ccctggcgaa actggcgacg ccatttgccg aaggtgaggc actgcgtctt tccagcgttt
    12781 cgcatccgga atacattgga cgcgtcgtga agttctttag cgatatcggc gggtgggcgt
    12841 tactaatgca tggtactgaa ggcgaagtgt atgctaatcc gcagcgctgc ccgcagatca
    12901 atctcattga ccgtgaaggg atgcgggtgc tgtatgaaaa acaggaccct gctggtagcg
    12961 agttactgcc acaagcaaaa gatccggaaa ccacggcgca gtggactgag cgttgcttgg
    13021 ctggcagcga agcgattccc gaatcgctga aaatccagat ggcttgctgc ctggtggcta
    13081 cgggtgaagc ggcaactatc agcgatggcc tggcgcgcgt taatcaggca ttttgatttt
    13141 tatctcagcc ggatgagcca cgtcatccgg cttttacccc accgcaatat gaaattcctg
    13201 catctttatt gaccttcccg cgcccggcgt gcagcataaa aaaacaacaa acacataaca
    13261 taaacaggag ttaaccatgg aaagtggtca tcgctttgat gctcagacgc tgcacagttt
    13321 tattcaggct gtatttcgtc agatgggtag cgaggaacaa gaagcgaaat tagttgccga
    13381 tcatttaatc gcggcaaacc tggcagggca tgattctcat ggtattggca tgatcccaag
    13441 ctatgtgcgt tcctggagtc aggggcatct gcaaattaac catcatgcca aaatcgttaa
    13501 agaggcgggg gcggcagtca cgctcgatgg cgatcgcgca ttcggtcagg tcgcggcaca
    13561 cgaagcgatg gcgctgggga ttgagaaagc gcatcagcac ggtattgctg ccgtggcgct
    13621 acataactcg catcatatcg gccgtatcgg ttactgggcg gagcagtgtt cagcggcggg
    13681 gtttgtctct atccactttg ttagcgtggt cggtattcca atggtcgcgc cgttccacgg
    13741 tcgcgacagc cgctttggca ccaatccctt ctgtgtggtt ttccctcgta aagataattt
    13801 cccgctgttg cttgattacg ccaccagcgc cattgcattt ggcaaaaccc gcgtcgcctg
    13861 gcataaaggc gtccccgtgc cgccaggttg cctgattgac gttaacggcg tgccgacgac
    13921 caatccggcg gtaatgcagg agtcgccgtt ggggtcgctg ttgacctttg ccgaacataa
    13981 aggctacgcc cttgcagcga tgtgtgaaat tcttggcggg gcgctttctg gcggtaaaac
    14041 gacgcatcag gaaacgttgc aaaccagccc cgatgccatt cttaactgca tgaccactat
    14101 catcatcaac ccggaactgt tcggcgcgcc ggattgtaac gcgcagaccg aagcctttgc
    14161 cgagtgggtg aaagcctcgc cgcatgatga cgataagccg attttgctac cgggcgagtg
    14221 ggaagtgaac acgcgtcgcg aacggcagga gcaggggatt ccattggatg cgggaagctg
    14281 gcaggccatt tgtgatgcgg cgcggcagat tggtatgtcg gaagagacgt tgcaggcttt
    14341 ctgtcagcag ttagccagct aaaaaaaagc ccgtccagtg gcggacgggc aaacaagggt
    14401 aacataggat caatgagggt tagagcatat gcgtctgtcg gcaaacagac agggaaatac
    14461 ttgtgctgga cgtagcgtaa acgcctgatc cgtccaacgg ttcggatttt gtaggcctga
    14521 taagacgcgg tagcgtcgca tcaggcatct gttgccggat gcggcataaa cgccttatcc
    14581 gtcctacgaa ttacttataa attaccgcag taccgcttaa tttattgttg ttggtggcgg
    14641 aagtgatgct gtatccactg gcaccggctg ccgccgcttt ctcagccagt ttcgcttcca
    14701 gggcatcgag ggtggatgcg ccatcggcag aaaccacgcc gattttattc atgttctgtg
    14761 cctgggatgc cgttaccggt tccgccgcga acacgccaaa tgacagagtt gaaagagcca
    14821 tagcagcaac aacagtattg atagttttca taattaatct ctcgcaggtg atttttgtat
    14881 aagtgacgtt gtttcgtcga tgtgatgagt atcacgtttt tttacgagag ataaaatcga
    14941 agagaattga ccgccttgtt caaataaatt gattgatatc taacatactg ataaaccatt
    15001 tatttgttgt acttatggac ttgttttatg ccgcgtggca gtcacagcag cgttaaaggc
    15061 acggaatgcc acgcaaaata caaattatac tcgctatgca tctcgccagt tgtagttaac
    15121 gtaactggct gtctttcgaa cctctgcgat tatatcctgt atacgctggt ttttgtaaat
    15181 ctttctcctg ctgacaatga atacataagc gcacgccagg aatggcttcc cgacgggctt
    15241 gcgggatggg ggcaccgcac tcttcacatt catacaggct ttcgccacgc ggaatttcac
    15301 cccgagcgcg ggcaatcgca tcttcaattg tactgttgat ctgttcgttg acggcgtcat
    15361 cgttagccca agcggatgcc atatcgtcct ccccatatca atgcttgtac agttaagtgt
    15421 agctaatata gggacgaacc tgggcagttc aagcgtcaga tctccgacca ttcccgcagc
    15481 agattatgat aaagattgag cagcgacagg atctcttcat tttcaccata gcggcttttc
    15541 agcgactgaa tatttttgtc cagttcaaac agcatggcgc gctttttatc atcgcggatc
    15601 atcgactgga tccacataaa tgatgccact cgtacgccgc gggttacggg tgtcacgcaa
    15661 tgcaggctgc tggagggata caacaccaga tcacctgccg ggagttttac ccgatgttgt
    15721 ccaaaggtgt cattaacgac cagttcgccg ccgtcgtagc tttgtggatc gcttaaaaac
    15781 agcgtggcag aaaggtcagt gcgcatccag ccgttttgtg gatggctgcg tactgcgcca
    15841 tccacatgga aaccataggt ttcattgttc tgatagcgat taaacagcgg cgtggaaagg
    15901 gtacgcggca aggcagcggc aaagaacaat gcatgttggt taaccgcgtt cagcacctca
    15961 ttttgcagtg cggcgtataa cgcgctgcgg gtgtctactt gctgattgtt cttaacttgt
    16021 gcaccttgtg ctccggtggt gacgcgtcca tccacccatt cggcttgttc cagttgttcg
    16081 cgaaaatggg cgacgtcctg tggcgataac acgccgggaa tgtggtacat catacaagtt
    16141 ctccaaaaag tggggcctgc gccccacatc tgaatcagaa atgcatattg gctgtgagca
    16201 agaaggttct tggctcgccc gggtgataac ggtagccgct cttgttgatt gaggcgacgt
    16261 aatcggtatc aaacaggttg taaacgttta gctggaagtc gagattgcga ttaacccgat
    16321 accccagttt ggcatcggcg acccagtaac cttctgtaaa tgctggcgtt cccaccgcgc
    16381 cgtctgaacc tttatgtata ctgccgatat agcgtgcgcc cgcgccaaca gagatatcat
    16441 cggttgcctg atattggctc cataaggtga aggcgtgctc cggggtatac ggcagcgatg
    16501 aggaaccatc ctgggcaaca tctttgccgt ttttgatggt tgctttttgc tgggtatagc
    16561 cgccaatcac ctgccacgcg ggagtgatat tccctgccac ggatatctca tagccttcga
    16621 cgcgtttctt accgtattgc gagtaagtcc cgtcatcatt ttgctcaact tcattttcga
    16681 tatcagtacg gaacagcgcg gcggtgagca acaggcgttt atccagaacc tgccatttgg
    16741 tgccaatctc gctggtgttg gctttttgcg gtttaaaatc ggtgcggttg gcactgttac
    16801 cactgccaga ctgcgcaagg gcgaagttgt tgccgcccgg aggctgctgg gaaacggcat
    16861 agttaatata gacattgccg ttttccgtca ggtgatacag tgccccggct ttccagttca
    16921 ccagattgcc cgacttggcg gtgtcgacgg tggtgactgg agaacctttt gccacaccag
    16981 ctgggcaggt gatggcaccg cgtccgctgc cgccgcaggc ggtggcactg tcatattcag
    17041 tatgataatt atccagacgg atcccgccgt tcagctcaaa atcacgggtg atttgcagcg
    17101 tatcgaaggc gtaaattgcg aaggtatccg tctgaccatt ggcgtttgcg ccgttgcgcg
    17161 tcaggccgcc gggatgaatt ctgctgtcag gatgataaat atttaccgcc ggtaacgtca
    17221 ccggattaac gccatagtta gtctgcgttt cacgggtaaa ttccacgccg gtactgacat
    17281 catgaccgat agaaccggta tagaacgtcg aggtcaggtt ggtctggttg gtgagaattt
    17341 tattactcac atctttggta ttcgtcgtgc gtgactaggt ccagctattc acatcgctgg
    17401 tgggctgagt aatattcgac gccccgccca taatcgccgt catcaggtaa tcctgcttta
    17461 cgcgcgacca acgggtagta ttgcgaatgg tggtgttatc gttgatgtcg tgctcaaaac
    17521 gcatggtggc ggtgtcggtg gtcgaatcgt cgtaatcgga atccgtgccg taaaagttat
    17581 gagtatcaac ttttccggaa tgattcaggg ccgccgctcc cgcagatggg gcagaatagc
    17641 ccggcaaacc gatggtcgga atgccgccgt ctggcgtgtt gtgctgagtg acatgcagat
    17701 aattaagata caaacgattc gctgtaccaa ggccaaaagc gacagaaggg gcgacgccgt
    17761 aacgctcatt tttgactttg tcgcgtccgg catcgtgggt tttttcgccc atcacattca
    17821 ggcgcac
//
LOCUS       DBKEKI010000071        17613 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.071, whole genome shotgun sequence.
ACCESSION   DBKEKI010000071 DBKEKI010000000
VERSION     DBKEKI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.071"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..848
                     /locus_tag="ACUQPX_002489"
     CDS             <1..848
                     /locus_tag="ACUQPX_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHV9057176.1"
                     /translation="KEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGA
                     QLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPA
                     ALKEAPDDATFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTF
                     NPAHAQQKIASADLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDA
                     QLRKADPVAWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWMNALEQEWLR
                     RYGTH"
     gene            821..2357
                     /locus_tag="ACUQPX_002490"
                     /pseudo
     CDS             821..2357
                     /locus_tag="ACUQPX_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            2357..3010
                     /locus_tag="ACUQPX_002491"
     CDS             2357..3010
                     /locus_tag="ACUQPX_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHV9057177.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            3077..4384
                     /gene="ynjE"
                     /locus_tag="ACUQPX_002492"
     CDS             3077..4384
                     /gene="ynjE"
                     /locus_tag="ACUQPX_002492"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HHV9057178.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVENEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(4393..5013)
                     /locus_tag="ACUQPX_002493"
     CDS             complement(4393..5013)
                     /locus_tag="ACUQPX_002493"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HHV9057179.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            5100..5507
                     /gene="nudG"
                     /locus_tag="ACUQPX_002494"
     CDS             5100..5507
                     /gene="nudG"
                     /locus_tag="ACUQPX_002494"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HHV9057180.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(5473..5745)
                     /locus_tag="ACUQPX_002495"
     CDS             complement(5473..5745)
                     /locus_tag="ACUQPX_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HHV9057181.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            5981..7324
                     /gene="gdhA"
                     /locus_tag="ACUQPX_002496"
     CDS             5981..7324
                     /gene="gdhA"
                     /locus_tag="ACUQPX_002496"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HHV9057182.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSIGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(7441..8481)
                     /locus_tag="ACUQPX_002497"
     CDS             complement(7441..8481)
                     /locus_tag="ACUQPX_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HHV9057183.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(8609..10570)
                     /gene="topB"
                     /locus_tag="ACUQPX_002498"
     CDS             complement(8609..10570)
                     /gene="topB"
                     /locus_tag="ACUQPX_002498"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HHV9057184.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSVAIA"
     gene            complement(10575..11618)
                     /gene="selD"
                     /locus_tag="ACUQPX_002499"
     CDS             complement(10575..11618)
                     /gene="selD"
                     /locus_tag="ACUQPX_002499"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HHV9057185.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(11735..12286)
                     /locus_tag="ACUQPX_002500"
     CDS             complement(11735..12286)
                     /locus_tag="ACUQPX_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HHV9057186.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            12447..14303
                     /gene="sppA"
                     /locus_tag="ACUQPX_002501"
     CDS             12447..14303
                     /gene="sppA"
                     /locus_tag="ACUQPX_002501"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HHV9057187.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSPHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            14470..15486
                     /gene="ansA"
                     /locus_tag="ACUQPX_002502"
     CDS             14470..15486
                     /gene="ansA"
                     /locus_tag="ACUQPX_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HHV9057188.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPRGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            15497..16138
                     /gene="pncA"
                     /locus_tag="ACUQPX_002503"
     CDS             15497..16138
                     /gene="pncA"
                     /locus_tag="ACUQPX_002503"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HHV9057189.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16231..17590)
                     /locus_tag="ACUQPX_002504"
                     /pseudo
     CDS             complement(16231..17590)
                     /locus_tag="ACUQPX_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         4261 a         4638 c         4477 g         4237 t
ORIGIN      
        1 taaaagaggc caatttatta caaacgggct gggcggagac tctgcccaac tggcgctatg
       61 tcgacacaca gctgccagtg cgggaagatt tttcagtgcc gacacaaggt gcggaatcgc
      121 cctggggcgg cgcacaactg acgtttatcg cccgccgcga tgttacgcca cagccaccac
      181 aaacgccgca agccttactg gagtttgcta aagccaatcc cggcacggtt acttatccgc
      241 gcccaccgga ctttaccggc acggcgtttc ttgaacagtt gctgattatg ctgacgccag
      301 atcccgccgc attaaaagaa gcgccggacg atgcgacttt cgcccgtgtc actgcgccct
      361 tgtggcaata tcttgatgca ctgcatccgt atttgtggcg cgaaggaaag gatttcccgc
      421 cttcgcccgc gcggatggat gctctgctga aagccggcac attgcgcctg tcgctgacct
      481 ttaaccccgc gcatgcgcag caaaaaatcg ccagcgccga tttgccggca agcagttaca
      541 gttttggctt tcgcgagggg atgattggca acgtgcattt cgtcaccatt ccagccaacg
      601 cgaatgccag tgctgcggcg aaggtagttg ccaatttcct gctctcaccc gatgcgcaac
      661 tgcgtaaagc agatcccgtt gcctggggcg atccttctgt tctcgatccg caaaaactgc
      721 ctgacgggca gcgcgaaata ttgcaatcaa gaatgccgca ggatctgccg ccggtactgg
      781 ctgaaccgca cgcaggatgg atgaatgcac tggaacaaga atggttacgc cgttacggta
      841 cgcattaatc tttttgctgt gggcgatggt ggcggtgatt tatgcaccgc tgatcccggc
      901 agctctcacg ctgatatcgc ctgcgttgtc gttgacacgc tggcaggcgt tatttgccga
      961 tccacagtta ccgcaggcat tactggcaac gctggtgtcg acaaccatcg cggcggtcgg
     1021 ggcattgttg attgccctgc tggtgattgt ggcgctatgg cctgggccga aatggcagcg
     1081 tatgtgcgcc cgtctgccgt ggctgctcgc cattccccat gtggcttttg ccaccagcgc
     1141 cctgctgctc tttgctgacg gagggctgct atatgactat ttcccgtatt tcactccgcc
     1201 aatggaccga tttggcatcg gtctgggcct cacccttgca gtgaaagaaa gcgcatttct
     1261 gctgtggatc ttagcggcag tattgagcga aaaacggctg ttgcagcagg tcattgtgct
     1321 ggattcgctg ggctacagcc gctggcaatg cctgaactgg ctgctgttgc cctccgtcgc
     1381 gccttcgctg gcaatggcga tgctggcgat tgttgcctgg tcgctttcgg tcgtggatgt
     1441 ggcgattatt cttgggccag gtaatccacc gacgctggca gtaattagct ggcagtggtt
     1501 aactcagggc gacgcggatc aacaaacgaa aggcgcactc gccagtctgc tgctgatgct
     1561 gttactcgcc gcctacgttt tgctgagcta tctgctatgg cgcagctggc ggcgcactat
     1621 tccccgcgta gatggcattc gcaatgcctg ccacaccttt attaccaggc actacgctgg
     1681 cgagtttttt acccttaacc ggtgtgctgt gtgtggttct gctggcgatc ctcgcggatc
     1741 agtcgacgat caatagtgaa gcactcatca acagtctgac aatggggctg gtagcaacat
     1801 tcatcgcttt gctcctgtta ctgctgtggc tggaatgggg ggcacagcgt cgccagttgt
     1861 ggctatggtt gcccattcta ttgcccgctc tgccactggt ggcgggccag tacacgctgg
     1921 cgctatggct gaacctggat ggaagctgga cggcggtggt ctgggggcat ctgctgtggg
     1981 taatgccgtg gatgctgttt atcctgcaac ccgcctggca gcgcattgat tcacggttaa
     2041 ttttgattgc gcaaacactg ggctggtcgc gggccaaaat cttcttttac gtgaaatgcc
     2101 cactcatgtt gcgccctgtg ctgattgcct tcgcggtggg attttcagtc agtattgcgc
     2161 agtatatgcc gacgctgtgg ctgggcgcgg ggcgttttcc gacgctcacc actgaggcgg
     2221 tggcattaag cagcggcggc agcaacggta ttctcgccgc ccaggcttta tggcaactgt
     2281 tgttaccgct tattattttt gccctgaccg cgttagtcgc aaaatgggta ggttatgtca
     2341 gacaaggaat ccgctaatgc tctgcgtgaa aaatgtttcg ctacgtttac cagaaagccg
     2401 cttgctgaca aacgttaact ttacggttga taaaggtgac atcgtcacgt taatggggcc
     2461 gtctggctgt ggaaaatcca ctctgttttc atggatgatt ggtgcactgg ccggacagtt
     2521 ttcttgtaca ggtgagctat ggctcaatga gcaacggatt gacatgctac ccaccgcaca
     2581 gcgtcagatt ggcattcttt ttcaggatgc actgttattt gaccagttca gcgtcgggca
     2641 aaatttactg ctggcgctac cggcgacact taaggggaat gcccgacgta atgccgtgaa
     2701 tgatgcactt gagcgttcag gccttgacgg agctttccat caggatcctg ccactttgtc
     2761 tggcggtcag cgagcgcgcg ttgctctact acgcgccctt ctcgcccagc caaaagcgtt
     2821 actcctggat gagccattca gccgtcttga tgtggctctg cgcgataatt ttcgccagtg
     2881 ggtgttcagc gaagttcgcg ccctggcgat ccccgtcgtt caggtaacgc acgatctcca
     2941 ggatgttcct gctgatagtt ctgttctgga tatggcgcag tggtcagaaa attacaacaa
     3001 actgcgataa cgcaaagttt ttctcaatgc gtcagttcag aatggcgcac tcaaaactac
     3061 aatgtcggga ttttcgatga aacgtgtttc tcaaatgacc gcgctggcaa tggctttagg
     3121 gctggcttgc gcttcttcgt gggccgctga actggcgaag cctcttacac ttgaccaact
     3181 tcagcaacaa aatggcaaag cgatagatac tcgccccagc gcgttttata acggctggcc
     3241 acaaacctta aatggccctt ctggtcatga acctgccgcc ttaaacctct ctgctagctg
     3301 gctcgacaaa atgagcaccg aacagctcaa cgcgtggatc aagcaacata acctgaaagc
     3361 cgatgccccg gtggcgctgt acggtaatgg caaagatgtc gacgccgtca aaacgcgact
     3421 gcaaaaagca ggttttacgc atatctccat tctgagtgac gcgctaagcg aaccttcccg
     3481 tctacaaaaa ctgccgcact ttgagcagct ggtttatccg caatggctgc atgacctgca
     3541 acaaggtaaa gaggttacgg cgaaacctgt cggtgactgg aaagtcattg aagcggcctg
     3601 gggcgctcct aagctttacc ttatcagcca tattcctggc gctgactaca tcgataccaa
     3661 cgaagtggaa aacgaaccgc tgtggaacaa agtttctgat gaacaactaa aagcgatgct
     3721 ggcaaaacac ggcattcgcc atgacaccac ggtcattctg tacgggcgtg acgtatacgc
     3781 tggagcgcgt gtggcgcaaa ttatgctcta cgcaggcgtg aaagatgtgc gtctgctgga
     3841 tggcggctgg caaacctggt ccgacgcggg actgcctgtt gagcgaggaa cgccaccgaa
     3901 agtgaaagcg gaaccggatt tcggcgtgaa gatcccggca caaccgcagt tgatgctcga
     3961 tatggaacag gcccgtggac tgctgcatcg ccaggatgca tcgctggtga gcattcgttc
     4021 gtggccagaa tttatcggta cgaccagcgg ttacagctat attaaaccaa aaggtgaaat
     4081 agccggagca cgttggggac acgctggtag cgactcgacg catatggaag atttccataa
     4141 cccggatggc accatgcgta gcgccgatga tattaccgct atgtggaaag catggaatat
     4201 caaaccagat cagcaagttt cattctactg cggcaccggc tggcgcgcgt ccgaaacctt
     4261 tatgtacgca cgcgccatgg gttggaagaa tgtttccgtg tatgacggcg gctggtacga
     4321 atggagcagc gatccaaaaa atccggttgc aaccggtgaa cgcggcccgg gcagtagcaa
     4381 ataacattgc aattactgac gctggagcga cttcagcgtc agatacccac tccacacccg
     4441 cgtaaaggtc gtcatccagc acaacgcacc gaagatccac gcaaaccacg caaaccatgc
     4501 agggaataaa catcccagca caaacagtaa gatcgtttct gtgccttcgg ttaatccccc
     4561 cagatagtaa aacgacttat gtgcataacc ggggttatca atctgatgtt ttgctgccag
     4621 tgcagcaaag gcgagaaaac tgctgcccgt gccgataaac gcaaacaaca accagccgcc
     4681 tgccagcgca ttttgctccg gtgcagcaag aataaagcca aacggcacca gcgcgtaaaa
     4741 gagaaaatcg agagaaatat caagaaagcc gcccgcgtcg gtaagccctc tgcgccgcgc
     4801 cagcgcaccg tccagaccat caagcagcct gttcaacaaa atgacgacta gcgccgccag
     4861 ataccagccc agcgccagaa acggcagagc cagcacgccg atggcaaatc ccactaatgt
     4921 taaaccatct ggcgtaatgc ccggtttatc aagaacccgc acgcactgat gcaacaacgg
     4981 tttaatccgg ggatgaagat ggcggtctag cacaggcact ccttaaatat aaagcctttc
     5041 tgattgagca acagtgcgga tattatggca tttttcgctt atctgcccgt gtgtaattta
     5101 tgaaaatgat tgaagttgtt gccgccatca ttgaacgtga tggcaaaatt ttactcgcgc
     5161 aacgccccgc ccagagcgat caggcgggat tatgggagtt tgccggtggt aaagtcgagc
     5221 tggatgaaag ccagcagcag gcgctggtgc gtgagttaaa cgaagaattg ggcatcgaag
     5281 caactgtggg tgagtatgtt gccagccatc agcgagaagt ttcgggccgg attatccatc
     5341 ttcatgcctg gcacgtaccc gacttccacg ggacgttaca ggcacatgaa catcaggcgc
     5401 tggtctggtg ctcacctgaa gaggcgctgc aatatccgct ggcccctgct gacattccat
     5461 tattagaggc gtttatggct ttacgcgccg ccagagcagc ggattagtgc taagggtttt
     5521 gtcatcacgc tggcattgca gcagtattcc ttcggcttta attaccgccc cttcagaata
     5581 attttgatcc tgataaacgc agcactgagt acagggctgc gctgactgcc cgcctgaact
     5641 gaacacttct ggcggtacgt ttacctccac gtccggacga taatgcgggt tagccagtgc
     5701 aattaatgga aatgctaata ctacggcgaa caatgctcga ctcacaggga actccttaac
     5761 gttattgtct ctgctactga taacggtagc cgggtggcaa aactttagcg tctgagttat
     5821 cgcatttggt tatgagatta ctctcgttat taatttgctt tcctgggtca tttttttctt
     5881 gcttaccgtc acattcttga tggtatagtc gaaaactgca aaagcacatg acataaacaa
     5941 cataagcaca atcgtattaa tatataaggt ttttatatct atggatcaga catattctct
     6001 ggagtcattc ctcaaccatg tccaaaagcg cgacccgaat caaaccgagt tcgcgcaagc
     6061 cgttcgtgaa gtaatgacca cactctggcc ttttcttgaa caaaatccaa aatatcgcca
     6121 gatgtcatta ttggagcgtc tggttgaacc ggagcgcgtg atccagtttc gcgtggtatg
     6181 ggttgatgat cgcaaccaga tacaggtcaa ccgtgcatgg cgtgtgcagt tcagctctgc
     6241 catcggcccg tacaaaggcg gtatgcgctt ccatccgtca gttaaccttt ccattctcaa
     6301 attcctcggc tttgaacaaa ccttcaaaaa tgccctgact accctgccga tgggcggtgg
     6361 taaaggcggc agcgatttcg atccgaaagg aaaaagcgaa ggtaaagtga tgcgtttttg
     6421 ccaggcgctg atgactgaac tgtatcgcca cctgggcgcg gataccgacg ttccggcagg
     6481 tgatatcggc gttggtggtc gtgaagtcgg ctttatggcg gggatgatga aaaagctctc
     6541 caacaatacc gcctgcgtct tcaccggtaa gggcctttca tttggcggta gtcttattcg
     6601 cccggaagct accggctacg gtctggttta tttcacagaa gcaatgttaa aacgccacgg
     6661 tatgggtttt gaagggatgc gcgtttccgt ttctggctcc ggcaacgtcg cccagtacgc
     6721 tatcgaaaaa gcgatggaat ttggtgctcg tgtgatcact gcgtcagact ccatcggcac
     6781 tgtagttgat gaaagcggat tcacgaaaga gaaactggca cgtcttatcg aaatcaaagc
     6841 cagccgcgat ggtcgagtgg cagattacgc caaagaattt ggtctggtct atctcgaagg
     6901 ccaacagccg tggtctgtac cggttgatat cgccctgcct tgcgccaccc agaacgaact
     6961 ggatgttgac gccgcgcatc agcttatcgc caatggcgtt aaagccgtcg ctgaaggggc
     7021 aaatatgccg accaccatcg aagcgactga actgttccag caggcaggcg tactgtttgc
     7081 accgggtaaa gcggctaatg ctggcggtgt agcaacgtcg ggcctggaaa tggcacaaaa
     7141 cgctgcgcgc ctgggctgga aagccgagaa agttgacgca cgtttgcatc acatcatgct
     7201 ggatatccac catgcctgtg ttgagcatgg tggtgaaggt gagcaaacca actacgtgca
     7261 gggcgcgaac attgccggtt ttgtgaaggt tgccgatgcg atgctggcgc agggtgtgat
     7321 ttaagttgta aatgcctgat ggcgctacgc ttatcaggcc tacagatggg cacaattcat
     7381 tgcagttacg ctctaatgta ggccgggcaa gcgcagcgcc cccggcaaaa tttcaggcgt
     7441 ttatgagtat ttaacggatg atgctcccca gggaacattt cttatgggcc aacggcattt
     7501 cttactgtag tgctcccaaa actgcttgtc gtaacgataa cacgcttcaa gttcagcatc
     7561 cgttaacttt ctgcgatagc agcagatatg ccagtaaaga aatcccatta gactattttt
     7621 ttgataatct tcttcgcttt cgaacaactc gtgcgccttt cgagaagcaa gcattatata
     7681 atgccaggcc agttcttctt caattgtccc gttttgaaaa gctgtgcttg atatcgagat
     7741 catccatgat aattccgccg cccatattag cttcgccgag gatttaccgg agcgattatt
     7801 agcgcaatca gagatatagt ctgagggaaa aacagcaaat ttattcaaca aggcgataac
     7861 ctgctctggg gcttcctcca tgtttgcttt aaaggtattg gctccatggt cgccagaaag
     7921 aaaatgctcc attaaggcac aataactttc gctatcttcg ataccccatt gattctctaa
     7981 agactcgcgt cttttactga tgatatcgat cgagtcaaaa ggaagcacat gatattggaa
     8041 ggtatctttg ccaggttcag gctttcgcgg ccagaactct agcgtttcag accattgctt
     8101 atgatagaat cgataaggtg cgatcaattg tagcgcctgt aacttctcga tactgagcgg
     8161 ctcaatacct ttagcctgat aataatgcag ttgttctttt tttgctttaa aaccggcccg
     8221 aacaataagc cccatcataa ttaatagata aagaaaagag catcccgcgg taatcaggcc
     8281 tctttcattc aaaccgttgg atgttatcgc tgcgaataca aacattacag cgacaacaca
     8341 tgttaaataa aacccccact tacaaagcag catggcctta ttttctttaa tcacccgttc
     8401 aaaattacta ttaaatattt cccagccatt aaaagaatac ttctcgctcc caggatggtt
     8461 ttgtaataaa acttttttca tcacattccc tgttattaca tactttagag gcgataattt
     8521 tatccatgca aaaaaaatat ccaacgaatt ttcttgatcc gctggatatt tcagaatatg
     8581 actcgaatag cacgaaagat tcgctcgctt acgctatcgc cacgcttccg gcttcatctg
     8641 ctggcggact ttttttcgca ctacgtttac gcggtgcagc ctttttctta tcagcactgc
     8701 caccactgcc cggagccaca atgccgcgaa actgccgcac cggcgtacgt ttggcttgat
     8761 caataagctg atatagcgtc cccaccagcg gctgcataaa gtcctgatag cgacactgct
     8821 tttcgctgat ttgcgtcagc accgattccc agtgcgcggt catgtccggt cgcgtcgcca
     8881 tttctggcag cgaatggaat aacgcttttc cggcgtcggt ggagtggata tagcgccctt
     8941 ttttggtcag aaaaccacgc ttgaacagca gttcaataat cccggcacgg gttgcttctg
     9001 tccccagacc atcggtcgca cgaaggacct ttttcagatc tttgtcctgc acaaagcgcg
     9061 cgatcccggt catcgccgaa agcagtgttg catcggtaaa atggcgcggc ggctgggttt
     9121 gccgctctac cacttcacct ttttcacaca gcaactcatc gcctttcgcc accacaggca
     9181 atggcgtgcc gtcgttttct tcatcgcgct ctttgctgcc taacagcgtg cgccagcctg
     9241 cttcagcaag aaaacgggct ttagcgacaa atttgccttt ggcaatgtcc agttcgataa
     9301 cacacttgcg gaacaccgca tccgggcaga actgcatcag gtattgacgg gcaatcaggt
     9361 tatagacctt cgcttcgttc tccgtcaggt tgatcgcaga actccgtgcg gtcgggatga
     9421 tggcgtggtg cgcatcgacc tttttgtcat cccaacagcg gttgcgtata tctggatcta
     9481 ccactggctg cggcaacaga tccggtgcat gaacgctgat ggcattcatc accgcgtggc
     9541 gtccggcaaa atgttcttct ggcaaatagc gacaatcgga acgcggataa gtgattagct
     9601 tgtgcgtttc gtacagcttc tggcagatat caagcacgtt ctgcgcgctc agaccgaagc
     9661 gttttgccgc ttcaatctgc aacgccgaaa gcgaaaacgg cagcggcgcg gattctgatt
     9721 cccgtttatc gttatagctg gtgacaatag ccggttgacc actaatgcgg ttaaccacat
     9781 gctccgccag tggacgatgt aacaagcgcc cttcttcatc ctggtacggt tcacacgctt
     9841 cgctcggttg ccagatagcg gtaaaccgct catcggcagg tgtcacgata tgtgctttga
     9901 cttcaaagaa gtctttcgcc acgaagtttt caatctcttc atcgcggcgc accaccagcc
     9961 cgagcacggg tgtctgcacg cgtcccacgg aaagtacgcc ctgataaccg gcattgcgac
    10021 cgagaatggt atacgcacgg gtcatattga tgccgtacag ccagtcggca cgcgctcgcg
    10081 ccagcgcaga aacgcacaat ggcacaaact cactgttgga acgaagacgg tcgatcgccc
    10141 gctcaaccgc ctgcgggttc aggtcgttta tcaagcaacg ctgcacctgc tggcgctttt
    10201 ccggtgccag ttgcagatag tccagcactt catccaccag caattgccct tcacgatccg
    10261 ggtccccggc gtgaacgatt tcgctggctt catgcaggaa gcgtttgatg acgttaagtt
    10321 gtttggtcac ggagggtcgt ggctgtaatt gccacttttc cgggacaatc ggcaaatccg
    10381 caagattcca gcgcgcatag cggctgtcgt aggcgtctgg ctgcgcctgc tcaagcaggt
    10441 gaccgataca ccaagtcacc acctgaccat taccgcactc gataaagcca tcgcctttcc
    10501 ggtgcggttt gggcaggaca tcagcaatgg cgcgcgccag actcggtttt tcggcaataa
    10561 acaaccgcat tgaattaacg gatctcaacc atggcacgac cgccgcgcgc tggcaccagt
    10621 tcgccaatcg ccgtcagttc aatgccaaac tcggcggctg tagttttaac ctcattttct
    10681 gcttccggca tgaccgccag cagcaaaccg ccagaagttt gcggatcgca cagcagatcg
    10741 cgcacttcac gcggcatttc acccatcaga tgaccgtagc tggcaaagtt acgttcagtg
    10801 ccgccaggta ctgcgcccaa cttaatgtac tcttcaacac cggggagttt cgggatcgct
    10861 tcatagtcga cgcgtgcctg cacaccagcc ccctgacaca tttcgctcaa gtggcccagc
    10921 agaccaaagc ccgtaacgtc ggtcatcgct tttacgcctt cgatgttggc aaaggacgcg
    10981 cctgcgatgt tcatccggca catcacttcc gtcgccagtc cctgatgttc tggtttcaac
    11041 agtgattttt tctcagccgt ggtaagaacg ccgatcccca gcggtttcgt caggaacagt
    11101 ttgcatccgg cttgtgcggt actgtttttc ttcacccgct cggtcggtac gatccccgtt
    11161 accgccagac caaaaatcgg ctccggcgca tcgatggagt gaccgccagc cagcgcaata
    11221 cccgcctgac ggcatgcata gcgtccacct tcggttactt cgcgggcaat ttccggggaa
    11281 agtttgttaa tcggccagcc gaggatcgca atcgccataa tcggtttgcc ccccatcgcg
    11341 aagatatcgc tgatggcgtt agtcgccgca atgcggccaa aatcgaaagg attatcaacg
    11401 atcggcataa agaagtcggt ggtactgata acgctggtgc cattgcccag atcgtacacc
    11461 gccgcatcgt cgcgggtttc attacccaca agcaaattcg gatcaacaaa cttcgcctgc
    11521 tcactgtgca ggatggtttc caacactttt ggggaaattt tacagccgca accagctccg
    11581 tggctgtatt gggtcaaacg aatcgagttc tcgctcatgg acatctcctg tcaatgcaat
    11641 ccgggtatgg taaccctcat tccgtgaagt gataagtgag agtgtctgaa ttcctgcgcc
    11701 tttgctcaca atccagacag tttcgcgaca attatcagaa ataagttaca aacggcgtcg
    11761 ggtccgggac gttaatcgac gtagatgctt tcaactgcgg cgtgccgagg tagagaaaac
    11821 cgacaatttt atcctgctca cggcaaccga atgcttcacg cactaccgga ctttcagtta
    11881 atgcgccact gcgccagatg ccgccaaatc cctgggcaac ggctgccatt tgcatcgcca
    11941 tgaccgcgca tccggcagac atttcctgtt cccatcgcgg gactttatga ttttctttgc
    12001 atttcgccac caccgtgatg atgagcggtg cgcggaacgg cgcattacgg gctttgtcga
    12061 tagctttatc atcactaccg gcagcaatcg ccccctgttc cagtacggcg ctgaaacgct
    12121 cgcacccttc cccttcaatc acaaaaaaat gccacggttg catggactta tggtccggcg
    12181 cacgcatacc cgcacgcagg atgttttgca gttgttcacc cgttggcgcg ggttcagcca
    12241 ggcgggaggc gctacggcga ttgatcaata gttcgagtgc atccatttga ttaactcctg
    12301 tcgtgatatt tattcacaaa attaacacga gagtggattt tgttacagca cagtccgcaa
    12361 ttcctgctga caagtgccgg ttgggtcatt acgataacca catctattgc gcctgtgaca
    12421 ggtgtgacct taagttggga gaatacatgc gaaccctttg gcgatttatt gccggatttt
    12481 ttaaatggac gtggcgtctg ctgaatttcg tccgtgaaat ggtacttaac ctgttcttta
    12541 ttttcctcgt actggttggt gtggggatct ggatgcaggt cagtggtggt gattcgaaag
    12601 aaacggccag tcgtggcgca ctgctgctgg acatttctgg tgtgatcgtc gataaaccag
    12661 acagttctca gcggtttagt aaattaagcc gccagctgct tggtgccagt tccgatcgtc
    12721 tgcaggaaaa ctcactgttt gatatcgtca acaccattcg ccaggcgaag gacgaccgca
    12781 atatcaccgg tatcgtgatg gatctgaaaa acttcgcagg cggcgaccag ccgtctatgc
    12841 agtacatcgg caaagccctg aaagagtttc gtgacagcgg gaaaccggtt tatgccgttg
    12901 gcgagaacta cagccagggg caatattatc tcgccagttt cgccaataaa atttggctgt
    12961 caccgcaagg cgtggttgat ctgcacggtt ttgctaccaa cggtctgtac tacaaatcgt
    13021 tgctggataa gctgaaagtt tccccccatg tgttccgcgt gggtacgtat aaatctgccg
    13081 ttgaaccgtt tattcgtgat gatatgtcac cggcagcccg cgaagctgac agccgctgga
    13141 ttggtgagct gtggcaaaac tatctgaata ctgttgccgc taaccggcag atccctgctc
    13201 agcaggtatt ccctggcgcg caagggttgc ttgagggttt aaccaaaacc ggtggcgata
    13261 ccgcgaaata tgcactggaa aacaagctgg tcgatgcact ggcatcgagt gccgaaatcg
    13321 aaaaagctct gaccaaagag ttcggctgga gtaagactga taaaaattat cgcgccatca
    13381 gttattacga ttacgcattg aaaacgccgg cagataccgg tgacagcatc ggtgtcgtct
    13441 tcgctaatgg cgcaattatg gatggcgagg aaactcaggg gaatgttggc ggtgatacca
    13501 ctgcggcaca aatccgcgac gctcgccttg acccgaaagt gaaagcgatt gtcctgcgtg
    13561 ttaatagccc aggcggcagc gttaccgcgt ctgaagtgat tcgcgctgaa ctggcagcag
    13621 cccgggcagc gggtaagtct gtggttgtat cgatgggcgg catggcggca tctggtggtt
    13681 actggatttc cacgccagct aattacattg tggctaaccc cagcaccctg accggttcta
    13741 tcggtatctt cggcgtgatc accaccgtag aaaatagtct ggattcgatt ggtgttcata
    13801 ctgatggtgt ctcaacttca ccgctggcgg atgtttctat caccagggca ctgccgccgg
    13861 aagcgcagca gatgatgcaa ttaagcattg agaatggcta taaacgcttt atcacgctgg
    13921 ttgctgatgc gcgtcattcg acgccggagc aaattgataa aatcgcccag ggccacgtct
    13981 ggaccggtca ggatgcaaaa gctaacgggc tggtcgatag tctcggggat ttcgatgatg
    14041 cggttgccaa agcagcagag ctcgcaaaag tgaaacagtg gcatctggaa tactacgttg
    14101 atgaaccgac cttcttcgac aaagtgatgg acaacatgtc tggttctgtc cgggcaatgt
    14161 tgccagatgc gttccaggcc atgttacctg caccgcttgc ctcggtagcc tctaccgtta
    14221 aaagtgaaag cgacaagctg gccgcgttta acgacccaca aaaccgttat gcgttttgcc
    14281 tgacctgcgc caacgtgcgt taagtcttgt actgagtggc cgacagatcg tcggccacat
    14341 tattttttac ctcgacgaat cctcttcccg ctgtctcgcc ccatatttcc ttatccgcat
    14401 agtatcaggt gcgctcctcc tgcctcacgt atatactttt gctctttcga tatcattcat
    14461 atcaatatca tgcaaaagaa atcaatttac gtcgcctaca cgggcgggac catcgggatg
    14521 cagcgttccg agcagggtta tattccggtg tcaggtcatc tacaacgcca actggcgctg
    14581 atgccggaat tccatcgccc ggagatgcca gatttcacca ttcatgaata tacgccgttg
    14641 atggattctt ctgatatgac gccggaagac tggcagcata ttgctgaaga tattaaagcg
    14701 cactatgacg actatgatgg ttttgtcatt ctacacggta ccgacacgat ggcgtatacc
    14761 gcctctgcgc tgtcgttcat gctcgagaat ctcggtaaac cggtcattgt gacagggtca
    14821 caaatcccgc tggctgagtt acgctctgac ggacaaatta atctgctgaa tgcgttgtac
    14881 gttgcggcga attatccgat caacgaagta acgctctttt tcaataaccg attgtatcgc
    14941 ggcaaccgca ctaccaaagc ccatgccgat ggttttgatg cgtttgcctc tccaaacctt
    15001 cctccgttac tggaagctgg tatccatatt cgtcgtttga atacgccacc cgccccgcgc
    15061 ggtgaagggg aattaatcgt tcatccaatc acgccacaac caattggcgt agtgacgatt
    15121 tatccaggga tttctgctga cgtcgtgcgc aattttctgc gccaaccggt gaaagcattg
    15181 attctgcgct cctatggcgt gggtaatgcg ccacaaaaca aagccttcct gcaggaatta
    15241 caagaagcca gcgatcgcgg tattgtggtg gtcaacctga cacaatgtat gtccggtaaa
    15301 gtgaatatga gtggttatgc caccggtaac gccctcgccc atgccggcgt tattggcggt
    15361 gcagatatga ctgtagaagc cacgctaacc aaactgcatt acctgctgag ccaggaactg
    15421 gatactgaaa ccattcgcaa ggccatgagc caaaacctgc gcggcgaact gacgccggat
    15481 gattaaggag actgcaatgc cccatcgcgc cctgttactg gtcgatttac aaaatgattt
    15541 ctgtgctggt ggcgcgctcg ccgtaccgga aggtgacagt acggtggagg ttgctaaccg
    15601 ccttattgac tggtgccagt cgcgcggaga agcggttatc gccagccagg actggcatcc
    15661 ggcgaatcac ggcagctttg ccagccagca cggcgtagag ccttatacgc caggccaact
    15721 cgacggcctg cggcaaactt tctggcctga acactgtgtg cagaacagtg aaggcgcaca
    15781 attacatccg ttactgaatc aaaaagagat cgcagcggtg ttccataaag gcgaaaatcc
    15841 tttagttgac agttacagtg ccttttttga taacggacgt cggcagaaaa ccgcgcttga
    15901 tgactggttg cgcgatcatg aaatcgatga attgatcgtt atgggcctgg ctactgacta
    15961 ttgcgtgaag tttaccgtgc tggacgcgtt acagttaggt tataaggtaa acgtgattac
    16021 cgatggctgt cgtggcgtga atatccagcc ccaggacagt gcacacgcgt ttatggagat
    16081 gtcagcagct ggggcaacgc tatatacgct ggcagactgg gaagagacgc aggggtaatt
    16141 ttacgctggc ctacaattct gtactggcat tgtaggccaa ataaaacacg tcagtggtac
    16201 atctggcaat tgatgccatc aacgaaagat ttaattcacc tcagaaatct cttccagcga
    16261 caccttccgc gtttcgatgc caaaaatgga gagaaccagc gcacagagca ctaacatgac
    16321 accaagcacc ataaacaccg tgatcgaccc ataatgtgtt aataatgccg caacgccata
    16381 gggcgtgaaa actgcgacga tccgtccgac ggcattaacg aaacccgaac cgcgcaggcg
    16441 taaatgcgtt ggccaaagct ccgggatata aaccgccgac gcgaagcaaa catacatgta
    16501 taaaaagaag atcatcacca gaccatagat taaaatcgcc cactctgtag tctgacttga
    16561 atagatatag cctaacacgg caataataat aagtaaggcg gagccaaata atcgacgagg
    16621 aaaatgatca ataattaatg ccgcaataaa tattcctacc ggagcaccaa tcataataac
    16681 agcggtcatt aatattgatt tatcgacatc aatgccggag ttaacaaata tggtcggtat
    16741 ccatacggta atggtataaa gcgaaatgtt cattgcaatt aaaacagtta tcgcaactaa
    16801 tgtacgtcgt aacatttccc ctttaaacag gagccagaag gtacccttga ttaccttaac
    16861 cttgctgttg ctctggttca aagtaagctg gggtaaacga atactcttct ccctttcaat
    16921 ttgctgctct acttcacgaa gttggctttc tgcaccggcg atttgccctt tccctgccag
    16981 ccatcgtggc gactcgataa agtatttacc tgagagaaac caggctaaca gtatgccaat
    17041 accacccaga agaaacatta ttcgccaact aaaaaaaagc gataaccacc acgcctatcg
    17101 ccgcagacag catgggcgac cagttaccaa caaatgagag ccgcgcggac cattttccac
    17161 gcaccttcgc ggggataaac tccgtaaatg aggcatagcc aaccataatc agcgccccca
    17221 ttcctgttcc catcaggaag cgaaagaaaa tgagccagta catatcaggg acaaaagcgg
    17281 cccctgttgc agcaataccg acgatgagaa gatttatgcg aaacgccctg cgccgcccaa
    17341 agtagtcacc aataaaccca ccagtaagtg agccgatgaa ataaccaaac attaatgccg
    17401 aggtaaaggc ggcattgagg aaattatttg accatccatt gcttaccagc tttgctaaga
    17461 cgacattacc tgagtaactc aaaaaccccg ttaataacag actaaagctt attataccaa
    17521 atatacgata atgaaaccgg gccaaaggca agcggtccag tcttgcgcca atttgatcat
    17581 attgttccat tgggtgcctc ggatttgcta aaa
//
LOCUS       DBKEKI010000072        17591 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.072, whole genome shotgun sequence.
ACCESSION   DBKEKI010000072 DBKEKI010000000
VERSION     DBKEKI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.072"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            46..648
                     /gene="ais"
                     /locus_tag="ACUQPX_002505"
     CDS             46..648
                     /gene="ais"
                     /locus_tag="ACUQPX_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HHV9057190.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSEGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(687..1112)
                     /gene="nudI"
                     /locus_tag="ACUQPX_002506"
     CDS             complement(687..1112)
                     /gene="nudI"
                     /locus_tag="ACUQPX_002506"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HHV9057191.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1391..1933
                     /locus_tag="ACUQPX_002507"
     CDS             1391..1933
                     /locus_tag="ACUQPX_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HHV9057192.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2020..3222
                     /locus_tag="ACUQPX_002508"
     CDS             2020..3222
                     /locus_tag="ACUQPX_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HHV9057193.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPPEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGNEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3286..3747
                     /locus_tag="ACUQPX_002509"
                     /pseudo
     CDS             3286..3747
                     /locus_tag="ACUQPX_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3788..4690)
                     /locus_tag="ACUQPX_002510"
     CDS             complement(3788..4690)
                     /locus_tag="ACUQPX_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HHV9057194.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPISFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(4883..6073)
                     /gene="glpC"
                     /locus_tag="ACUQPX_002511"
     CDS             complement(4883..6073)
                     /gene="glpC"
                     /locus_tag="ACUQPX_002511"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HHV9057195.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6070..7329)
                     /gene="glpB"
                     /locus_tag="ACUQPX_002512"
     CDS             complement(6070..7329)
                     /gene="glpB"
                     /locus_tag="ACUQPX_002512"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HHV9057196.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRVTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7319..8947)
                     /gene="glpA"
                     /locus_tag="ACUQPX_002513"
     CDS             complement(7319..8947)
                     /gene="glpA"
                     /locus_tag="ACUQPX_002513"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HHV9057197.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEETGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFLLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGGRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9220..10578
                     /gene="glpT"
                     /locus_tag="ACUQPX_002514"
     CDS             9220..10578
                     /gene="glpT"
                     /locus_tag="ACUQPX_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HHV9057198.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10583..11659
                     /gene="glpQ"
                     /locus_tag="ACUQPX_002515"
     CDS             10583..11659
                     /gene="glpQ"
                     /locus_tag="ACUQPX_002515"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HHV9057199.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11701..11943)
                     /locus_tag="ACUQPX_002516"
                     /pseudo
     CDS             complement(11701..11943)
                     /locus_tag="ACUQPX_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            11938..12042
                     /locus_tag="ACUQPX_002517"
                     /pseudo
     CDS             11938..12042
                     /locus_tag="ACUQPX_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12105..12761
                     /gene="inaA"
                     /locus_tag="ACUQPX_002518"
     CDS             12105..12761
                     /gene="inaA"
                     /locus_tag="ACUQPX_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004096779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HHV9057200.1"
                     /translation="MTGDSSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGK
                     KLYVKCMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRA
                     LLVTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRH
                     IYVKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM
                     "
     gene            complement(12815..13069)
                     /gene="yfaE"
                     /locus_tag="ACUQPX_002519"
     CDS             complement(12815..13069)
                     /gene="yfaE"
                     /locus_tag="ACUQPX_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HHV9057201.1"
                     /translation="MARVTLRITGTQLQCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(13069..14199)
                     /gene="nrdB"
                     /locus_tag="ACUQPX_002520"
     CDS             complement(13069..14199)
                     /gene="nrdB"
                     /locus_tag="ACUQPX_002520"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HHV9057202.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHQTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14433..16718)
                     /gene="nrdA"
                     /locus_tag="ACUQPX_002521"
     CDS             complement(14433..16718)
                     /gene="nrdA"
                     /locus_tag="ACUQPX_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HHV9057203.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            16846..16989
                     /gene="ypaB"
                     /locus_tag="ACUQPX_002522"
     CDS             16846..16989
                     /gene="ypaB"
                     /locus_tag="ACUQPX_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HHV9057204.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            17414..>17591
                     /locus_tag="ACUQPX_002523"
     CDS             17414..>17591
                     /locus_tag="ACUQPX_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057205.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSI"
BASE COUNT         4171 a         4273 c         4685 g         4462 t
ORIGIN      
        1 ttcacggtaa ttgtctggtt gcgcttgtct ataggtggag tttacgtgtt agctttttgc
       61 cgctcatcat tgaagtcaaa aaaatatttc atcattttac tggcgctcgc tgcaattgcc
      121 ggactgggta ctcatgccgc ctggagtagc aatggtttgc cacgtatcga caataaaaca
      181 ttggccagac tggcacagca gcacccggtt gtcgttttgt ttcgccatgc tgaacgttgc
      241 gatcgttcaa ccaatcaatg cttgtcagat aaaacaggta ttacggttaa aggtacccag
      301 gatgcccgtg aactgggcaa cgcttttagc gctgacatcc ctgatttcga tctttattcc
      361 agtaataccg tccggaccat tcagtcggcg acctggtttt ccgagggtaa aaagctgacg
      421 gtagataaac gacttcttca gtgcggtaat gagatttata gtgcaattaa ggacttacaa
      481 agcaaagcgc ctgataaaaa tatcgttatt ttcacccata atcattgcct gacatatatt
      541 gctaaaaata agcgtgatgc gacatttaaa cctgattatc tggatggttt agtcatgcat
      601 gtggaaaaag gcaaagttta tctggatggg gaattcgtta accactaaat tttcaatctg
      661 acagccagtg atggctgtca tcattgttaa agaagacctt tcaaacgtaa cgtttttcgg
      721 gtggcaacat ttaaatcata atgcaccaga tcttcaggtt ttacccacgc gtagtcctga
      781 aactcttcgt ttattttcac ttctcggttg gcagaaacgc agtcaaaaat caggtaaatc
      841 atataaatct cttccttgcg accatctgca tatgtcttgg tgcgaatatc atcgctgaag
      901 gtccacggcg tgatttctgt caaaagcagc tgttctccca gttcttcgcg aatttcgcgg
      961 cgtagtgcct cttcaattcg ttcgccagac tccacgccgc cacccgaaag cgcccattga
     1021 ccggggaaaa cgccgcgatc gtcggccatt ttgcacagca aataagcacc atcattttga
     1081 atcaaagggc atacaatagt ccgttgtcgc acaggggtat tccttataaa taaaaagaaa
     1141 ccatgagaat acggtaactc agaaatatgt gaaagtgaca gtaatctctt ttgcaatgat
     1201 aactgcactt attattattt ttgtgtgtat cggtaaagtg tgtttatttt ttcggcgttt
     1261 aattatttaa gaagtattta gttacaataa gtgactagat cccttttttc gctcattgaa
     1321 tcctctgtgc caggcagtac aatcccgcag ctttgggaat tttgattaat tgcttgtggg
     1381 aaaggggata atgaaaaaaa ttgcgctagc aggtctggcc ggaatgctgt tggtttctgc
     1441 atcggtcaat gcaatgagca tcagcggcca ggcgggtaaa gaatacacca atattggtgt
     1501 cggttttggg actgaatcga cgggcctggc tttaagcggt aactggacac ataacgacga
     1561 cgacggtgac gtcgcgggcg tggggctggg gttgaatctg cctctcgggc cgttaatggc
     1621 gaccgttggc ggaaaaggcg tgtacaccaa cccgaattac ggcgatgaag gttatgccgc
     1681 agcggtagga ggtggtttgc agtggaaaat tggcaacagc ttccgtttgt ttggcgagta
     1741 ttactactct ccggattcgc tctccagcgg tattcaaagt tatgaggaag cgaatgctgg
     1801 cgcgcgttac accattatgc gtccagtcag tattgaggcg ggttatcgct acctgaatct
     1861 gtcgggtaaa gacggtaacc gcgacaacgc cgtggctgac ggcccgtatg ttggggttaa
     1921 cgccagtttc tgatcccttc cggcgcggca ttcagtttct tactacgcct tatctccctt
     1981 tgcgttatag tgattcgata acaaaagcag gagataacca tgttaaaagt ggaaatgcta
     2041 tccaccgggg atgaagtgtt atacgggcaa atcgttgaca ctaacgctgc ctggctggcc
     2101 gattttttct ttcatcaggg gctgccatta tctcgccgca atacggtggg ggataacctt
     2161 gatgacttag tcaccattct tcgcgaacgt agtcagcacg ccgatgtgct gatcgttaac
     2221 ggcgggctgg gaccgaccag cgatgattta agcgcactcg ccgctgcgac agcaaaaggt
     2281 gaaggcctgg tgctgcatga agcctggctc aaagagatgg aacgctattt ccacgaacgt
     2341 ggacgagtaa tggcaccgag caaccgtaaa caagcggagc tgcctgccag tgctgaattt
     2401 atcaataacc cggtaggcac cgcctgtggt tttgccgtgc agcttaatcg ttgcctgatg
     2461 ttctttactc ccggcgtacc gccagaattt aaggtgatgg tcgagcacga aatcctgccg
     2521 cgcctgcgcg agcgtttttc tttaccacag ccgccggttt gtctgcgttt aactaccttt
     2581 ggtcgttcgg aaagcgatct ggcacaaagc ctggacactc tacaactgcc gccgggcgta
     2641 acaatgggct atcgctcctc aatgccgatc atcgaactga aactcaccgg accggcaagc
     2701 gagcaacagg cgatggaaaa actgtggctg gacgttaaac gtgttgccgg acagagcgtg
     2761 attttcgaag gcaacgaagg actgcccgcg cagatcagtc gcgaattgca aaaccgccag
     2821 ttcagcctga cgttgagcga gcaattcacc ggtggtttat tggctttgca actttctcgc
     2881 gcaggtgctc cattgctggc gtgtgaagtg gttccttcac aggaagaaac cctggcgcaa
     2941 actgcgcact ggattacaga acggcgggcc aaccattttg ccgggctggc actggctgtt
     3001 tcgggtttcg agaacgagca tctcaacttt gcgctagcca cgccagacgg cactttcgct
     3061 ctgcgtgtgc gtttcagcac tacacgctac agcctggcta tccgtcagga agtgtgcgca
     3121 atgatggcac tgaatatgct acgccgttgg ttaaacggcc aggacatcgc tagtgagcat
     3181 ggctggattg aggttgttga gtccatgact ttatccgtct gatttcgcca ctctgtgccg
     3241 gtttactgca ccggcacatt ccccctccgg caaaatttgt ccaaccctcc ctacggtgag
     3301 cgtggtgttg gggccagtgt ggcacgggct gcgcgctggg gacgcattga gaattacatg
     3361 gcgcaagtca acgattcgct ttgtctgtta gtgcaggtgg aaagtaaaac ggcactggat
     3421 aacctggacg agatcctcga cgtcgaaggg attgatggcg tgtttattgg accagcggat
     3481 ctttctgcgt cgttgggcta cccggataac gccgggcacc cggaagtgca gcgaattatt
     3541 gaaaccagca ttcggcggat ccgtgctgcg ggtaaagcgg ctggttttct ggctgtggct
     3601 cctgatatgg cgcagcaatg cctggcgtgg ggagcgaact ttgtcgctgt tggcgttgac
     3661 acgatgctct acagcgatgc cctggatcaa cgactggcga tgtttaaatc aggcaaaaat
     3721 gggccacgca taaaaggtag ttattgatat caaaggccca tggggatcgg ctgtgggcct
     3781 gtgttaatta gtggttattc gctgccagat cggcttcgct tagctgggtg gccgcgagca
     3841 cctggtcacg atcaatgccc tgttccagca tgcgcaaagc aattttaatg gcttgttcat
     3901 gcataccttc ctgccagcca atttgatgcc cttcctgccg tagccgttca gcaatagtca
     3961 ttaatctctc cttttgtagt ggtgaacgtt cggcaatctc ctgaataaaa cgggtgaaac
     4021 gggaggtatc gccgcattgc agcagataat taaacagtgt ttgtagctgg ctgtcattag
     4081 tgaagcctct aaccaaaagc gtggtgatcc tgtcgaccat gccgattaaa tcgtggtcgc
     4141 gaatatgctt ttgaatcagt tccagcagag ctatacgccg atgttgcatg atctcatcgt
     4201 caggtacgat ggtgatatcc accaacggaa aagcttcggt gtacaactgc cgggcaagtt
     4261 gcggatcgtc aaactcatcc agccagttaa gtgagtacgg gtagggcgat gtttcgccat
     4321 gataaaacag caatggcacc accagcggaa ctctgtcata gcctttatct aggtgacgct
     4381 gcatggcggc agtggcatag cgcattggcc gaaaagccat attcttttct gcagagcttt
     4441 gatgttcaat cacgcagtag atatagccgt caccgtcgct ggtttccacg gaccacaaaa
     4501 catccgagta gtaagcgcgt aaactttttt caataaaact aatgggttcc aggcgtaagg
     4561 tttgcaggtt gcaaagcttg cgcagtggtt ctggtaaatg tatttcgaga aaatcccgtg
     4621 cggtttcggg tgtgaatata aaggttttaa ataccgcatc atgcggggag gaggttgttg
     4681 attcggtcat ggttgtccgt cacctgatat ttatgatgac gcgacattac ggttatccca
     4741 ggcctttgac atctgccagt ttcagccttt gcgagacgtt ttcagaggaa ttttcactgc
     4801 gttgcaacgc atacattggg cacggaatcg tgtacgcaaa aatgcccggt aaccacatgc
     4861 ttaccgggca tcagaaggga gtttaagcca gcgcctgggc cagtagcgta atcggatgtt
     4921 cgcagcgaag acttgtggac atctcaatct gccatttaca ggtttcgcag tcggtgacca
     4981 ccagatctgc gccgctttct tctatctggc ggaacagtgg tgcgccgatg gcttgtgagg
     5041 tggggtagtt ctcttttttg aaaccgtaag tacccgcaat accgcagcac tgggaatcca
     5101 gcaccgttaa ctcaagcccc gggatgttac gcaacagctc cagggtgtag agcgtccagc
     5161 ccattttttc catatggcac ggagtgtgat aaaccacttt cagcggcagc ggtttcagcg
     5221 gtaacgtttt gccttcgtcc agcttgcgcc acagccagcg ggttgccagt tcgatatgat
     5281 cgcgcaagcc tttgttgtcg acattcagca cttccgggta ttcgtcgcgc agggcaaatg
     5341 tacaggttga ggaggtggca atcaccggaa tgccttttac tcccacagct tcgcggatcg
     5401 actctacatt cgtaattgcc tgtttgcgtg ctttatcggt aaagccgttg gcgattagcg
     5461 gtacgccgca gcatttttct ttgctgagca gttgtacacc ggtacccatt gcgttgagca
     5521 ctttaattaa atctttacct aactgcggat ggttgtagtt aacgaagcag ccgtgaaaga
     5581 aagcgacctg gtctttatat tgtgcttgct gagccgctat gctgcgatac cagcgacgga
     5641 acgtgccgaa ggagtatttc ggtagcgtgc gacgatgatc gatttttaac gccgcatcaa
     5701 gcagctgccg caccggtttc agcgaggtag cggtgttgac gattggtgcg aacggcgtgg
     5761 aaacgctacc catcaggtcg gtatgactca acacaaaatt acgcagcgac gggcgcgtgg
     5821 tgtcatattt cgcccgcgcg cgctggataa tatcgccaat cttcacatcg gacgggcagg
     5881 cgacttcaca acgtttgcag ttgatgcaat atttcagcgc ctcgtcatac agtgcgccat
     5941 ctttcaaacg cagacgctcg ccatccggcc cggcttgttt tggccctgga taaccgggat
     6001 tcaccctgct caccgggcag gcggtggtgc agacggtgca cttaatgcag ttttcgaagc
     6061 tggtgtcgtt cattgttggc ctcctgcgcg ttgggcaatc tgttgagcgg catgtaaagc
     6121 actgacggca caaacaccgc cgccgcatcc ctgggcgatg ggatcaaatc cgcccagcac
     6181 cgaaccgatg gcaaacaggt tctcaatggt ttgccctgcc tgcgacgggc gtagcgtctc
     6241 atcagtggtt acaccgaact gctgccacgg ttgcggcgca aaaaaatcgc ccttatacca
     6301 ttcaccccgc gtggcggttt gtagcacatc aaggccgaga atcggctcgc gaatgccgtt
     6361 acgttccgct accagtccgc cactaaagaa actgccgctg gcgagaaccg cgaaacgtgg
     6421 acgtagcgga atatcggcgt gattgcgggt ccagatttca ttcactacgc catttttaca
     6481 ggtcactttt ttcacttcat cgcccggcat ccacacgcca ccctggcgca caaactggcg
     6541 ctgtaactgg ttttgcagac gaatgcccag cacggaaggc ggcagcgttg gcaaaagcat
     6601 cagtgaacaa ggtagttttt cattcaacca acgccacagt ttgtcatcgg ccaggccgaa
     6661 gcaggcgggc atcaggatca tttcgcaggt attggcgaca ggaataagcg catcaagtaa
     6721 cagcggccag ttttcttcat tatcaaggaa acgggcgata ttcaccgcgc gaaattcggt
     6781 ggcgttattg cgcaggacat ccagttccgg cagctctatt tctgcggttt caaccgaaag
     6841 atccagttcg cgcaacgaag ccgccgcaag atgcgcctgg aaatccatca gcccgcttat
     6901 ccccactaca catatcttct tcgcgggtag cggccagacg ggaacttctg gcgaacttaa
     6961 ccaggttaca cgcaaagtgc cgagcggcgt aatccgctgg tgagccagtt ctacgctgcc
     7021 ctgcaattgc gcaccgcttt cagcgattaa tgcctgcgcc tggcaagcga gatcgagcac
     7081 gcgttgtggc ccgagaagag agtaaggatg tgcaggtgcc tgctgacgca aagattccag
     7141 tccactgtga atgtctgcca cgggttgacc atctggcaga tggctcagca aatccagcga
     7201 accggatgag aaatgcaacg cgctttgacc acgcgtgaca atggcacagc gcaggccgtg
     7261 tttttgcagt tgcaggccac agagtaatcc ggcgaggccg ccgcccataa tgacagtatc
     7321 aaagcgcatc tttctgctcc ttctccagac cacacagtcc ctgataaacc cagcgggtaa
     7381 attcgctttc gcgcagtgcg tctccccagg cgatgggttg cacgcctttc cagcgttcgt
     7441 taaggaaggt ggaaagttgc tcgatagatt gcgcggacgt cgtgacgtta aaccgttgca
     7501 gcagtccggc agcgcggcag gcgcagagtt cgccctggca ggtgcccatc cccacacggg
     7561 tacgacggcg taaatccagc aggctattaa cgtttaaatt ttctaccgcg tactgcactt
     7621 cacccgcagt taccgcttcg cactcacata ccaggctacg gtgcagacgg ccttcgctca
     7681 gccaggcagg cgtgcgatcg ccatgacgat aaaccgcaga accgcgcagc ggggcaggca
     7741 gggagatgac tttacgcaag gtaacttcag cgggttcttg tgaaccaggc agtgccagat
     7801 cggcagtcgt acaggggcgc gtgttgccca gtttgcggca taccgcgtcg gtagcccatt
     7861 cagccatcag ccgataggtc atcagtttgc caccggtgat ggtgataaat ccgtccagac
     7921 catcgcgttc agcatggtcg agcagcacga tgccacggct gacgttacgt ccgctcgggt
     7981 cgtcatcgct ggcaaccagc gggcgcacgc cagaataggc ccgcaaaatg cgcgttttcg
     8041 ccatcacggg ggccagtttt tccccttcac gcagcagaat atcaacctct tctgccgtca
     8101 ctcgattatc gtcaatctcg ttgtaatcaa tacgtaaaga ggtggtacca atcagcgaaa
     8161 tggtatcgcc aggcaccaga atatcggcgt cggaaggttt acggcagcgg ttgatcacat
     8221 gctggttaat gcggtgatcc atgatcagca gcgatccttt cgccgggaac atgcgaatgc
     8281 gcagatcggc atattcggca atgtgttgcc cccagatccc agcggcatta accacgacag
     8341 gtgcatgaag ggcctgagtt tcgccggtga gatggttacg tacacgacca ccgcacaccg
     8401 tcgcgccttc acgaatcagc cccgtgactt catgagcggt aaggataacg gcaccgtgtt
     8461 ctttggcatc cagcatgttt gctgcggtca gaagaaatgg atcaacggtg ccatccggaa
     8521 ctttcaccgc gccaatcagt gccgggttaa cggcaggttc gataatgcgc gcttgctgcg
     8581 ggtctatagc ttctgcgctg atccctgttt cttcgcaggc gcgaataaaa gtggcctgga
     8641 atgagaggtc atcttccggc agggtgataa acaggccgtt ggttggttca acgcagtgac
     8701 gtgcaatgcg tttcaggatc tggttttcac taatgcattc gcgggccgat tccgcatcgg
     8761 ttactgcata gcgcgcaccg ctgtgcagca ggccgtggtt acgcccggtg gcaccggttg
     8821 cgatgtcgtg gcgctcaacc aaaatcacgc gcagcccgcg cagggcacag tcgcgggcaa
     8881 tcccggctcc cgttgcgccg ccgccaatga taatcacgtc acttgattgc gagtcgcgag
     8941 ttttcattgt tatccctctg aagttcgttt tttaccattt agccatagta aaaacataaa
     9001 ttgtttgatt tcgcgcatat tcgctcataa ttcgaaagtg aaacgtgatt tcatgcgtca
     9061 ttttgaacat tttgtaaatc ttatttaata atgtgtgcgg caattcacat ttaatttatg
     9121 aatgttttct taacatcgcg gcaactcaag aaacggcagg ttctctcact gaatcaggct
     9181 gttaatcata aataagacca cgggccacgg aggctatcaa tgttgagtat ttttaaacca
     9241 gcgccacaca aagcgcgctt acctgccgcg gagatcgatc cgacttatcg tcgattgcgc
     9301 tggcaaattt tcctggggat attctttggc tatgcggctt actatttggt tcgtaagaac
     9361 tttgcgcttg ctatgcctta tctggttgag cagggattct cacgcggtga tttaggtttt
     9421 gccctttcgg ggatctcgat tgcttatgga ttttcgaaat tcatcatggg ttcggtatcg
     9481 gatcgctcga atccgcgcgt tttcctgccc gcaggtttga ttctggcggc ggcagtgatg
     9541 ttgtttatgg gctttgtgcc atgggcgacg tcgagcattg cggtgatgtt tgtactgttg
     9601 ttcctctgcg gttggttcca ggggatgggg tggccgccgt gtggtcgtac tatggtgcac
     9661 tggtggtcgc agaaagaacg tggcggcatt gtgtcagtgt ggaactgtgc gcacaacgtc
     9721 ggtggtggta ttccgccgct gctgttcctg ctggggatgg cctggttcaa tgactggcat
     9781 gcggcgctct atatgcctgc tttctgcgcc attctggtgg cattattcgc ctttgcgatg
     9841 atgcgcgata ccccgcaatc ctgtggcttg ccgccgatcg aagagtacaa aaatgattat
     9901 ccggacgact ataacgaaaa agcggaacag gagctgaccg cgaagcaaat cttcatgcag
     9961 tacgtgctgc cgaacaaact gctgtggtac atcgccatcg ccaacgtgtt cgtttatctg
    10021 ctgcgttacg gcatcctcga ctggtcaccg acttatctga aagaggttaa gcatttcgcg
    10081 ctggataaat cctcctgggc ctacttcttc tatgagtatg cgggtattcc gggcaccctg
    10141 ctgtgcggct ggatgtcgga caaagtcttc cgtggtaacc gtggggcaac cggcgtcttc
    10201 tttatgacac tggtgaccat cgcgactatc gtttactgga tgaacccggc aggtaaccca
    10261 accgtcgata tgatttgtat gattgttatc ggcttcctga tctacggtcc tgtgatgctg
    10321 atcggtctgc atgcgctgga actggcaccg aaaaaagcgg caggtacggc agcgggcttt
    10381 accgggctgt ttggttacct gggcggttcg gtggcggcga gcgcgattgt tggctacacc
    10441 gtggacttct tcggctggga tggcggcttt atggtaatga ttggcggcag cattctggcg
    10501 gttatcttgt tgattgttgt gatgattggc gaaaaacgtc gccatgaaca attactgcaa
    10561 aaacgcaacg gaggctaatg gcatgaaatt gaagctgaaa aaccttagca tggcgatcat
    10621 gatgagcact atagtcatgg gaagcagtgc aatggcggcg gacagcaacg aaaaaatagt
    10681 catcgcccat cgcggtgcca gtggatattt gccggagcat acgctgccag caaaagcgat
    10741 ggcgtatgcg cagggagcgg attatctgga acaggatttg gtgatgacca aagacgacca
    10801 tctggttgtt ctgcatgacc attatctcga tcgtgttact gatgttgccg atcgtttccc
    10861 ggatcgggcg cgcaaagacg gtcgttacta cgcgatagat ttcacgctgg atgaaattaa
    10921 gtcgctgaaa tttaccgaag gtttcgatat tgaaaacggt aaaaaagtac agacttatcc
    10981 ggggcgtttc ccaatgggta agtccgactt ccgggtgcac acctttgaag aagagattga
    11041 atttgttcag gggttaaatc actctaccgg gaaaaatatc ggtatctatc cagaaatcaa
    11101 agcgccgtgg ttccatcatc aggaagggaa ggatattgcg gcaaaaacgc tggaagtgct
    11161 gaagaaatat ggttacaccg gtaaagacga taaagtttat ttgcaatgtt ttgatgctga
    11221 tgagctgaag cgtattaaga atgagctgga acccaaaatg ggcatggagc tcaatctggt
    11281 acagctgatt gcctataccg actggaatga aacgcagcag aaacagccgg atggaagctg
    11341 ggttaattac aactacgact ggatgtttaa gccgggtgcc atgaaacagg tggcggaata
    11401 tgcagatggt attggtccgg attaccatat gttgattgag gagacatcgc agccgggtaa
    11461 tatcaaactc actggcatgg tgcaagatgc tcagcagaat aaactggtag tgcatcctta
    11521 taccgtgcgg tcagataaac tgcctgaata cactcctgat gtgaatcagt tatatgatgc
    11581 tctgtataac aaagcgggtg taaatgggct gtttactgat ttccctgata aggcagtaaa
    11641 atttcttaat aaagagtaat gttactgcag gatggcgttc atgcgccatc ctgtctcata
    11701 tcaaattttt ccaatcgcca ctaattgttc catgcacatt tgtagtaaaa acagtggact
    11761 gccttctacc ggcttttctt tagtcagcaa cgaaatctgt cgatagaagg ttggttcgtg
    11821 aggaacggaa cacaattcac ctgcaatgct tttcagcgtt aactctggtt gtaaggcgat
    11881 gcctaatccc tgtcgaataa aattcatcgc tgtatccgac tgattaaatt cacattttat
    11941 ctgctgggcg cactattagt ggtagtggct ggtggaatct atgcgctgca aatgaagggc
    12001 aaaagcggtg tcacacctgt agcggcaaaa gaaattcatt aatacgacac gtttcattaa
    12061 gattttcctc aggtaaccac ctatagtcat tgtcgttaat aaaaatgaca ggcgatagtt
    12121 cagcaaagta cgacgaattt aatcactggt gggcaacaga gggcgactgg gttgaagagc
    12181 ctaactatcg ccgtaatggt atgagcgggg tgcaatgcgt cgagcgcaac ggcaaaaagc
    12241 tgtatgtaaa gtgcatgacg catcatctgt ttcattcagt acgttatccg ttcggccgac
    12301 caacgattgt ccgtgaggtt gcggtaatta aagaactgga acgggccggt gtcatcgtgc
    12361 cgaagatcgt ttttggtgaa gcggtgaaaa ttgagggtga atggcgcgcg ttgctggtga
    12421 ctgaagatat ggcggggttc atcagcattg ctgactggta tgcccggcat gcagtatcgc
    12481 cttattctga cgaagtacgg caagccatgt tgaaagcagt ggcactggcg tttaagaaaa
    12541 tgcatagcgt taatcgtcag catggctgtt gctatgttcg ccatatctat gtgaaaacag
    12601 aaggtaaggc agaagctggt tttcttgatc tggagaaaag tcgtcgccgc ttgcgtcgcg
    12661 ataaagcgat taatcatgac ttccgccagc tggaaaaata tctggagccg atccctaaag
    12721 cggactggga gcaggtgaaa gcgtattact acgccatgta atcgttgtag gccagataat
    12781 acgcgtaagc gtcgtatctg gcgctagtac ccgatcacaa ctcgatttca atatcgcctt
    12841 ttgcccggca acaacagggc aaaatttccc ccggctgaat aaaggctaac ggttcggcaa
    12901 tccagtccac ctgacctgcg accagtcggg tgcgacagga gccgcagtaa ccttcgcggc
    12961 actggtactc aaccgccaca ttgtgggatt ccagcgccgc cagaagggaa gggtgttcat
    13021 cctggcactg cagttgtgtg ccagtgatgc gcagggtaac gcgggccatc agagctggaa
    13081 gttactcaaa tcgtcggtgt ccacttccgc gtcaatctgc ccgaccagat aagaactgac
    13141 ttccacttcc tgcggagcaa cctgcacgtt atcagacacc agccaggtgt tgatccacgg
    13201 gatcgggttg gagcgcgtct ggaacggcag atccaaaccg actgcctgca tgcggatatt
    13261 ggtgatgtat tcaacgtact ggcagagaat atctttattc agaccaatca tcgaaccgtc
    13321 acggaacaga taatccgccc agtctttctc ttgctgtgcc gcctggacaa acaggtcata
    13381 gcactcctgc ttacattctt cggcaatttc cgccatctcc ggatcatccg cgccgctgcg
    13441 cagcagattc agcatatgct gagtaccggt ctggtgcagg gcttcatcgc gggcaatcag
    13501 gcgaataatc ttggcgttac cttccatcag ttcgcgttct gcaaaggcaa aggaacaagc
    13561 aaagctgacg tagaagcgga tcgcttccag cgcgttaacg ctcatcaggc agagatacag
    13621 ttttttcttc agctcgcgca ggctaacggt tacggtttta ccgttaacgg tgtgggtgcc
    13681 ttcgcccaga aggtgccagt agctggtcat ttcgatcagt tcatcgtaat agctggagat
    13741 cccttccgca cgtttctgga tctgctcgtt ggtgacgata tcgtcaaaca caacagacgg
    13801 atcgttaacg atattacgaa tgatatgagt ataggaacgg gaatgaatcg tttctgagaa
    13861 cgcccaggtt tcgacccagg tttccagttc cggaatagaa ataagcggca atagcgccac
    13921 gttcgggcta cgaccctgaa tggaatccag cagcgtctga tatttcaggt tgctgataaa
    13981 gatgtgtttt tcgtgctccg gcagcgcctg gtaatctata cggtcgcggg agacgtcaac
    14041 ttcttccgga cgccagaaga aagagagctg cttttcgatc agcttttcga agatgtcata
    14101 tttttgctga tcgtagcgag ccacgttgac cggctgacca aagaacatcg gttctttgag
    14161 ctgatcattt ttcgtctgtg aaaaggtggt atatgccatg agtgtgtcct gttgggagtt
    14221 taatgccgga taaggcattt tacgccgcat cctgtgcctg atgcgacgct ggcgcgtctt
    14281 atcaggccta caaatccgag ccgtaggccg gataaggcgt tcacgctgca tccggcaccc
    14341 ggagcctgat gcgacgctgg cgcgtcttat caggcctaca aaccgagccg taggccggat
    14401 aaggcgttta cgccgcatcc ggcatctcaa tatcagatct tacatgcgcc gctttcgcag
    14461 ccatcgtcct ggattgacgg taccagatcg tcctgcgcgt cttcagcgcc gtcacgggtg
    14521 ttctgataat acagcgtttt gaccccgaat ttgtaggcgg tgagcaggtc tttcagcaac
    14581 tgctgcatcg gcacttttcc tgacgggaaa cgtgacggat cgtagttggt gttagcagag
    14641 atcgactgat cgataaattt ctgcatgata cctaccagtt gcaggtagcc atcgttaccc
    14701 ggcatttccc acagcagctc ataggcgtcg tgcaggtgct cgtagtccgg caccacctgg
    14761 cgcaaaatac cgtctttcga cgctttgatg ctgacgtaac cgcgcggcgg ttcaataccg
    14821 ttagtggcgt tagagatctg cgaagaggtc tcggacggca tcagggcaga aagcgtggag
    14881 ttacgcaggc cgtgcgtttt gattgattca cgcaatgctt cccagtcgta atgcagcggc
    14941 tcattagcga tggcatccag atctttctta taggtatcga tcggcaggat ccctttcgcg
    15001 taagtggttt cgttaaacca cgggcacgcg ccttgctctt tagccagctc attagacgct
    15061 ttcagcaggt aatactgaat cgcttcgaag gttttatgcg tcaggttgtt ggcgctgccg
    15121 tcggagtagc gtttaccgtg cttcgccaga tagtaagcga agttaatcac accaataccc
    15181 agcgtacgac gccccatcgc tccacgtttg gcggccggga tcgggtaatc ctgataatcc
    15241 agtagcgcgt caagtgcacg aaccgccaga atcgccagct cttccagttc atccaggcta
    15301 ttaattgcgc ccaggttgaa agcagacagc gtacacagcg cgatttcacc gttctcgtcg
    15361 ttgacgtcgt tcagcggttt ggtcggcagg gcaatttcca ggcacaggtt agactggcga
    15421 actggcgcga tggtcggatc aaacggacta tgggtattac agtggtcaac gttctgaata
    15481 tagatacgac cggtagacgc acgttcctgc atcatcagcg agaacagctc aacggctttc
    15541 acacgctgct tgcggatgct gtcgtctttc tcatatttgg tatacaggcg ttcaaactct
    15601 tcctgatcgg cgaaaaacgc gtcgtacagc cccggtacgt cggacgggct gaacagggtg
    15661 atatcttcac ctttcagcag gcgggtatac atcagtttgt tgatttgtac cccgtagtcc
    15721 atatgacgca cgcggttgcc ttcaacccca cggttgtttt tcaacaccag caggctttcc
    15781 acttccagat gccacatcgg gtagaacagc gttgccgcac cgccgcgcac accgccctga
    15841 gagcaggatt tcaccgctgt ctggaaatgt ttgtagaacg ggatacagcc ggtatggaac
    15901 gcttcgccac cgcgaatcgg gctgcccagc gcacgaatac gtccggcgtt gatgccgatc
    15961 ccggcacgct gggaaacata tttcacaatc gcgctggagg tggcgttgat ggaatccagg
    16021 ctgtcaccgc actcgatcag tacgcaggag ctgaactgac gagtcggggt acgcacgccg
    16081 gacatgattg gcgtcggcag cgaaatttta aatgtggaaa ccgcgtcgta aaaacgcttc
    16141 acgtattgca ggcgcgtttc acgcgggtag ttcgagaaca ggcacgcggc aacaagaata
    16201 taaaggaact gggcgctttc gtagatttcg ccggtcacgc ggttctgtac cagatatttg
    16261 ccttccagct gcttaacggc agcataagag aaggtcatat cacggtcgtg atcgataaac
    16321 gtgtccatct gcttgaactc ttcttccgtg tagtcttcca gcagatgatt atcgtatttg
    16381 cccatctcga ccattttcac cacgtggtcg tacagcgcag gcggctcaaa ctggccgtag
    16441 gcttttttac gcaggtggaa gatcgccagg cgcgcggcga gatactgata atccggcgca
    16501 tcacgggaga tcaggtctgc ggcagccttg ataatagttt cgtggatgtc agaggtcttg
    16561 ataccgtcat aaaactgaat gtgggagcgc agctcgacct gggaaatcga aacgttatgc
    16621 agtccttctg ccgcccaatc cagaacgcga tggattttgt cgagattgat gcgctctgtg
    16681 ctaccgtcgc gctttgtcac cagcagattc tgattcatgt atgtcgtacc tgtttttgga
    16741 attctttccg caatagtttc atgcagacta cctgtagtaa gggaagctgc ttagaacact
    16801 atatataggg ggtatgtttg ggattcactg caagatagtg tgaaaatgac cctcttgcaa
    16861 gtgcataact ttgtggataa ctcaggaagg aaaaagtggc tttcgcgcac cttaggtcag
    16921 acaaggtgtc cgggaaagtc aatgggaaga gaaaaatttg ttaaaaataa ctgttcggcg
    16981 atttcttgaa cggcagaata ggttggataa attgatatag atggtttatt aatctttttg
    17041 ctaatgattg caaaaattga tctaagcacg agtcatcaca taatctgatg tatcagttgt
    17101 ggtcaatata taagcgaatg taagcgattg ttgatgtcga aatggttgtt ttctattcgg
    17161 ttattgttaa atgcgaagcc attttgttaa tttaccttat attataagaa aattattcat
    17221 tactttaatt tagtttaaat ataactggta tcccttaagg ttattaactt gaattatgac
    17281 gcactgatat tattcatcaa ataataacaa aatagccatt gcatcgggtt ggacaattta
    17341 caaaagaaag ataacccata ttgtataatg cgtttaaata tgccgtctta tataaaatga
    17401 tggtcagatt aatatgcgga ttatctttct acgcaaggag tatttatctc tactcccgtc
    17461 aatgattgca tctcttttct ctgctaacgg tgtcgcggcg gccattgatt tatgccaggg
    17521 atatgatatc aaagcgagtt gtcacgccag caggcaaagc ctttcaggca ttacgcagga
    17581 ctggagtatc g
//
LOCUS       DBKEKI010000073        17435 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.073, whole genome shotgun sequence.
ACCESSION   DBKEKI010000073 DBKEKI010000000
VERSION     DBKEKI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.073"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(8..67)
                     /gene="ibsA"
                     /locus_tag="ACUQPX_002524"
     CDS             complement(8..67)
                     /gene="ibsA"
                     /locus_tag="ACUQPX_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HHV9057206.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(339..395)
                     /locus_tag="ACUQPX_002525"
     CDS             complement(339..395)
                     /locus_tag="ACUQPX_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HHV9057207.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(667..723)
                     /locus_tag="ACUQPX_002526"
     CDS             complement(667..723)
                     /locus_tag="ACUQPX_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HHV9057208.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            1002..2243
                     /gene="mdtA"
                     /locus_tag="ACUQPX_002527"
     CDS             1002..2243
                     /gene="mdtA"
                     /locus_tag="ACUQPX_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HHV9057209.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSPFKVALAQTQGQLAKDKATLANTRRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            2243..5365
                     /gene="mdtB"
                     /locus_tag="ACUQPX_002528"
     CDS             2243..5365
                     /gene="mdtB"
                     /locus_tag="ACUQPX_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HHV9057210.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDQVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            5366..8443
                     /gene="mdtC"
                     /locus_tag="ACUQPX_002529"
     CDS             5366..8443
                     /gene="mdtC"
                     /locus_tag="ACUQPX_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HHV9057211.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPKALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            8444..9859
                     /locus_tag="ACUQPX_002530"
     CDS             8444..9859
                     /locus_tag="ACUQPX_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9057212.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVDMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            9856..11259
                     /gene="baeS"
                     /locus_tag="ACUQPX_002531"
     CDS             9856..11259
                     /gene="baeS"
                     /locus_tag="ACUQPX_002531"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HHV9057213.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            11256..11978
                     /gene="baeR"
                     /locus_tag="ACUQPX_002532"
     CDS             11256..11978
                     /gene="baeR"
                     /locus_tag="ACUQPX_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HHV9057214.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAVSYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            12169..12501
                     /locus_tag="ACUQPX_002533"
     CDS             12169..12501
                     /locus_tag="ACUQPX_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HHV9057215.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            12648..14009
                     /gene="yegQ"
                     /locus_tag="ACUQPX_002534"
     CDS             12648..14009
                     /gene="yegQ"
                     /locus_tag="ACUQPX_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HHV9057216.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(14351..14668)
                     /gene="yegR"
                     /locus_tag="ACUQPX_002535"
     CDS             complement(14351..14668)
                     /gene="yegR"
                     /locus_tag="ACUQPX_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HHV9057217.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            15074..15973
                     /gene="yegS"
                     /locus_tag="ACUQPX_002536"
     CDS             15074..15973
                     /gene="yegS"
                     /locus_tag="ACUQPX_002536"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HHV9057218.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAITIDMAQVNKQTCFINMPTGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(16055..16828)
                     /locus_tag="ACUQPX_002537"
     CDS             complement(16055..16828)
                     /locus_tag="ACUQPX_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HHV9057219.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(16934..>17435)
                     /locus_tag="ACUQPX_002538"
     CDS             complement(16934..>17435)
                     /locus_tag="ACUQPX_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HHV9057220.1"
                     /translation="VALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVL
                     RELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELT
                     VIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIARNAMPG
                     KVLLIP"
BASE COUNT         3960 a         4666 c         4611 g         4198 t
ORIGIN      
        1 ggtgttctta gtaagcctgg aagctaatca ctaagagtat caccagtatg atgacgtgct
       61 tcatcataac cctttcctta tttaaggccc cttcctcggg aggggctttc ccgtgtcagc
      121 gtcccgctga aatcgtcggc ttacctcctt tcgccctgaa tgcagtctat cgctaacgcg
      181 tgaacaaaat actttcctct gtgattattg cgtaacaact cgcaaagaat atttgctggc
      241 aggatcgcag actacaaagc ctacggattg acaatcttat cgtgaaggca taatttcagg
      301 agtgagggta gagcggggtt tcccccgcct tggtagtctt agtaagcggg gaagcttatg
      361 actaagagca ccacgatgat gagtagcttc atcatgaccc tttccttatt taaggcccct
      421 tcctcgggag gggctttccc gtttcagcgt cccgctgaaa tcgtcggctt acctcctttc
      481 gccctgaatg cagtctatcg ctgacttgcg gataaaatag tttccagtgt gattattgcg
      541 taacgtctcg caaagaatat ttgctggcag gatcgcagac tgcaaagcct gcagattgac
      601 aatcttatcg tgaaggcata ctttcaggag tgagggtaga gcggggtttc ccccgccctg
      661 gtagtcttag taagctggcc agctaatgac taagagcacc acgatgatga gtagcttcat
      721 catgaccctt tccttattta tggccccttc ctcgggaggg gctttcccgt ttcagcgtcc
      781 cgctgaaatc ggcggcttac ctcctttcgc catgcatgca gtctatcgct aacgcgtaga
      841 taaaatagtt tcctgtgtta ttactggatg cgtgctcgca aatgtgcccg ccattcagac
      901 gattcccgat agtgtttcat aattcctcca tttttctccc ttattggctg gctacactag
      961 tatcattccg cgaaacgttt caggaagaga aactcttaac gatgaaaggc agttataaat
     1021 cccgttgggt aatcgtggtg gttatcgccg ccatcgccgc attctggttc tggcaaggcc
     1081 gcaatgactc ccggagtgca gccccagggg cgacgaaaca agcgcagcaa tcgccagcgg
     1141 gtggtcgccg tggtatgcgt tccggcccat tagccccggt tcaggcggcg accgccgtag
     1201 aacaggcagt tccgcgttac ctcaccgggc ttggcaccat taccgccgct aatactgtta
     1261 cggtgcgcag ccgcgtggac ggtcaactga tggcgttaca tttccaggaa ggccagcagg
     1321 tcaaagcagg cgatttactg gcagaaattg accccagccc gttcaaagtt gcattagcac
     1381 aaacccaggg ccaactggca aaagataaag ccacgcttgc taacacccgc cgtgatctgg
     1441 cgcgttatca gcaactggca aaaaccaatc tcgtctcccg tcaggagctg gatgcccaac
     1501 aggcgctggt cagtgaaacc gaaggcacca ttaaggctga tgaagcaagc gtcgccagcg
     1561 cgcagttgca actcgactgg agccgcatca ccgcaccagt cgatggtcgc gttggtctca
     1621 agcaagttga tgttggtaac caaatctcca gtggtgatac caccggaatt gtggtgatca
     1681 cccagacgca tcctatcgat ttagtcttta ccctgccgga aagcgatatc gctactgtag
     1741 tgcaggcgca aaaagccgga aaaccgctga tggtagaagc ctgggatcgc accaattcga
     1801 agaaattaag tgaaggcacg ctgttaagcc tcgataacca aatcgatgcc acaaccggta
     1861 cgattaaagt gaaagcacgc tttaataatc aggatgatgc gctgtttccc aatcagtttg
     1921 ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt agtgatccca acagccgccc
     1981 tgcaaatggg caatgaaggc cattttgtct gggtgctgaa tagcgaaaac aaggtcagca
     2041 aacatctggt gacgccgggc attcaggaca gtcagaaagt ggtgatccgt gcaggtattt
     2101 ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct gaccgaaggg gcgaaagtgg
     2161 aagtggtgga agcccagagc gccaccactc cggaagagaa agccaccagc cgcgaatacg
     2221 cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg agcagcacag gcggcccgtc
     2281 gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg atggtggcga tcttactcgc
     2341 cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg ccggaagtgg actatccgac
     2401 cattcaggtg gtcacgctct acccaggtgc cagcccggat gtcatgacct ctgccgttac
     2461 cgcgccgctc gaacgccagt tcgggcagat gtctggcctg aaacagatgt cgtcgcaaag
     2521 ttccggcggt gcgtcagtta tcactttgca gttccagcta acattaccgc tcaatgtcgc
     2581 cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac ttgttgccga gcgatctgcc
     2641 taacccgccg gtttacagca aagtgaaccc ggcagatccg ccgatcatga cgctcgccgt
     2701 cacctcaacc gccatgccga tgacgcaagt ggaagatatg gtggaaaccc gcgtcgcgca
     2761 gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt tccggcggtc agcgtccggc
     2821 tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc ggcctgacca gcgaaaccgt
     2881 gcgcaccgcc attaccggcg ctaacgttaa ctcggcaaaa ggtagcctcg acggcccttc
     2941 ccgtgcggtc acgctttccg cgaacgacca gatgcaatcc gccgaagagt atcgccagct
     3001 aatcatcgcc taccagaacg gcgcgccaat tcgtctgggc gatgtcgcaa ctgtagagca
     3061 aggtgcagaa aacagctggc tcggcgcgtg ggcgaacaaa gaacaggcca ttgtgatgaa
     3121 tgttcagcgc cagcccggtg ctaacattat ctccaccgcc gacagcattc ggcagatgct
     3181 gccacagctc actgagagtc tgccgaaatc ggtgaaggtg acagtgcttt ccgatcgcac
     3241 caccaatatc cgcgcatccg tcgatgatac tcagtttgaa ttgatgatgg ctatcgcgct
     3301 ggtagtcatg attatctacc tgtttttgcg caatattccg gcgaccatca ttcccggtgt
     3361 tgctgtaccg ctgtcgttaa tcggcacttt cgcggttatg gtgtttctcg atttttcaat
     3421 caataacctg acactgatgg cgttaactat cgccaccgga ttcgtggtcg atgacgccat
     3481 cgtggtgatc gaaaacattt cccgctatat cgaaaaaggc gaaaaaccgt tggcggcggc
     3541 gctcaagggc gcaggtgaaa tcggctttac cattatctcg ctgaccttct cactgattgc
     3601 ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg cgactgttcc gcgaatttgc
     3661 tattaccctg gcggtagcga ttttgatctc agcggtggtg tcgctgaccc tgacaccgat
     3721 gatgtgcgcg cggatgctca gccaggagtc gttgcgtaaa cagaaccgct tctcccgtgc
     3781 ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt cgtggactgg cgaaagtgct
     3841 gaatcatccg tggctgacct taagcgtggc actcagcacg ctgctgctta gcgtgctgct
     3901 gtgggtgttc attccgaaag gtttcttccc ggtacaggac aacggcatta ttcagggcac
     3961 tttgcaggca ccgcaatcca gctcctttgc caatatggcc cagcgacaac gccaggtcgc
     4021 ggacgtgatt ttgcaggatc cggcagtgca aagcctgacc tcatttgttg gcgttgatgg
     4081 cactaacccg tcgctgaata gtgcacgttt acaaatcaac ctcaaaccgt tggatgaacg
     4141 tgatgatcag gtgcaaaaag tcatcgcccg tctgcaaacg gcggtagata aagtgccggg
     4201 cgtcgatctc ttcctgcaac caacgcagga cctgaccatc gatactcagg tcagccgcac
     4261 ccagtaccag tttaccttgc aggccacgtc actggatgcg ctcagtacct gggtgccaca
     4321 gttgatggaa aaactccagc aactgccaca gctttctgat gtctccagcg actggcagga
     4381 caaagggctg gtggcgtatg tcaatgttga tcgcgacagc gccagccgtc tggggatcag
     4441 catggcggac gtcgataacg ccctgtacaa cgcgtttggt cagcgactga tttccactat
     4501 ttatactcag gctaaccagt atcgcgtggt gctggagcac aataccgaaa ataccccagg
     4561 cctcgcggcg ctggatacca ttcgcctgac cagcagcgac ggtggcgtgg tgccgttaag
     4621 ctcaattgcc aaaattgagc agcgttttgc gccgctttcc atcaaccatc tggatcagtt
     4681 cccggtaacg accatctcct ttaacgtgcc ggataactat tcgctgggcg atgcggtgca
     4741 ggcgattatg gacaccgaaa agacgctgaa tctgccggtg gatatcacca cgcaattcca
     4801 gggcagcacc ctcgccttcc agtcggcgct gggcagcact gtctggctga ttgtcgcggc
     4861 ggtggtggcg atgtatatcg tgctcggcat tctgtacgag agctttattc acccgatcac
     4921 cattctctcg acgctaccca ccgcaggggt cggcgcgctg ctggcattaa tgattgccgg
     4981 tagcgaactg gatgtgattg cgattatcgg cattattttg ctgatcggta ttgtgaagaa
     5041 gaacgccatc atgatgatcg acttcgcgct ggctgctgag cgcgagcaag gcatgtcgcc
     5101 gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt ccgatcctga tgaccaccct
     5161 ggcggctctg cttggcgcgc tgccgctgat gttgagtacc ggggtcggcg cggaactgcg
     5221 tcgtccgtta ggtatcggca tggtcggcgg tctgattgtc agccaggtgc tgacgctgtt
     5281 caccacgccg gtgatttatc tgctgttcga ccgcctggca ttgtggacca aaagtcgctt
     5341 tgctcgccat gaagaggagg cgtaagtgaa gttttttgcc ctcttcattt accgccctgt
     5401 ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc atactgggtt tccgtatgct
     5461 gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt atggtcagcg cctcgctgcc
     5521 cggtgcgtcg ccggagacaa tggcgtcttc cgttgccacg ccgctggagc gctcacttgg
     5581 gcgcattgcc ggggttagtg aaatgacctc cagcagttcg ctcggcagca cgcgcattat
     5641 tttgcagttt gattttgacc gggatatcaa cggcgcagcg cgtgatgtgc aggcggcgat
     5701 caacgctgca caaagtttgc tgcccagcgg gatgcccagc cgcccgacct atcgcaaagc
     5761 gaacccgtcg gatgcgccaa ttatgatcct cacgctgaca tccgatactt attcgcaggg
     5821 tgaactgtac gatttcgcct cgacgcagct ggctccgacg atttcgcaaa tcgacggtgt
     5881 cggtgatgtc gatgtcggtg gcagctcgct gcccgccgtg cgcgtcgggc tgaacccgaa
     5941 ggcgctgttt aatcagggcg tgtcgctgga cgacgtacgc actgccatca gcaatgccaa
     6001 cgtgcgtaaa ccgcaggggg cgctggaaga tggcactcac cgctggcaga tccagaccaa
     6061 cgatgaactg aaaaccgccg ctgaatatca gccgctgatt attcactaca acaacggcgg
     6121 cgcggttcgt ctgggcgatg tggcgacggt gaccgactca gtgcaggatg tgcgcaacgc
     6181 cgggatgacc aacgccaaac cggctatttt actgatgatc cgcaaactgc cggaagccaa
     6241 tattatccag acggtagaca gcatccgggc aaaattacca gagttgcagg agaccattcc
     6301 ggcggcgatt gatctgcaaa ttgcccagga tcgctccccc accattcgcg cctcgctgga
     6361 agaagtcgag caaacgctga ttatctcggt ggcgctggtg attctggtgg tctttttatt
     6421 cctgcgctcg ggtcgcgcca ctattattcc cgccgttgcg gtgccggttt cgctgattgg
     6481 tacgtttgcg gcgatgtacc tgtgcggatt cagtctcaat aatctttcgc tgatggcgct
     6541 caccattgct actggtttcg tggtggatga cgccattgtg gtgctggaaa acattgcccg
     6601 tcatctggaa gcggggatga aaccactaca ggccgcgctg caaggtactc gcgaagtcgg
     6661 ctttacggtg ctgtcgatga gtctgtcact ggtggcggtg ttcctgccgc tgctgttgat
     6721 gggcggattg ccgggccgac tgttacgcga gtttgccgtg acgctttctg tcgccattgg
     6781 tatttcgttg ctggtttccc tgacgctaac gccaatgatg tgcggctgga tgctaaaagc
     6841 cagcaagccg cgtgagcaaa agcgactgcg tggttttggc cgcatgttgg tagccctgca
     6901 acaaggctac ggcaagtcgc tgaaatgggt gctcaatcat acccgtctgg tgggcgtggt
     6961 gctgcttggc accattgcgc tgaatatctg gctgtatatc tcgatcccga aaaccttctt
     7021 cccggagcag gacaccggcg tactgatggg tgggatacag gcggatcaga gtatttcgtt
     7081 tcaggcgatg cgcggcaaat tgcaggattt catgaaaatt atccgtgacg atccggcagt
     7141 ggataatgtc accggcttta ccggcggttc acgagttaac agtgggatga tgtttatcac
     7201 cctcaagcca cgcgacgaac gcagcgaaac ggcgcagcaa attatcgacc gtctgcgcgt
     7261 gaagctggcg aaagaaccgg gggcgaatct gttcctgatg gcggtacaag atattcgcgt
     7321 tggtgggcgt cagtcgaacg ccagctacca gtacacgttg ttatccgacg acctggcggc
     7381 actgcgcgaa tgggagccga aaatccgcaa aaaactggcg acgttgccgg aactggcgga
     7441 cgtgaactcc gatcagcagg ataacggcgc ggagatgaat ctggtttacg accgcgacac
     7501 catggcacgg ctgggaatcg acgtacaggc cgccaacagt ctgttaaata acgccttcgg
     7561 tcagcggcaa atctcgacca tttaccagcc gatgaaccag tataaagtgg tgatggaagt
     7621 ggatccgcgc tatacccagg acatcagtgc gctggaaaaa atgttcgtta tcaataacga
     7681 aggcaaagcg atcccgctgt cgtatttcgc taaatggcaa ccggcgaatg ccccactatc
     7741 ggtgaatcat cagggattat cggcggcctc gaccatctcg ttcaacctgc cgaccggaaa
     7801 atcgctctcg gacgccagtg cggcgatcga tcgcgcaatg acccagcttg gtgtgccttc
     7861 gacggtgcgc ggcagttttg ccggcacggc gcaggttttc caggagacga tgaactcgca
     7921 ggtgatcctg attatcgccg ccatcgccac ggtgtatatc gtgctgggta tcctttacga
     7981 gagttacgta catccgctga cgattctctc caccctgccc tcggcgggcg ttggagcgct
     8041 gttggcgctg gagctgttca atgccccgtt cagcctaatc gccctaatag ggatcatgct
     8101 attaatcggc atcgtgaaga aaaacgccat tatgatggtc gattttgcgc ttgaagccca
     8161 acggcacggt aacctgacgc cgcaggaagc tattttccag gcctgtctgc tgcgttttcg
     8221 cccgattatg atgaccaccc tggcggcgct gtttggcgcg ctgccgctgg tattgtcggg
     8281 cggtgacggc tcggagctgc ggcaacccct ggggatcacc attgtcggcg gtctggtgat
     8341 gagccagctc ctgacgctgt ataccacgcc ggtggtgtat ctctttttcg accgtctgcg
     8401 gctgcgtttt tcgcgtaaac ctaaacaaac ggtaaccgag taaatgacag atcttcccga
     8461 cagcacccgc tggcaattat ggattgtggc tttcggcttc tttatgcagt cgctggacac
     8521 caccatcgta aacaccgccc ttccctcaat ggcgcaaagc ctcggggaaa gtccgttgca
     8581 tatgcacatg gtcattgtct cttatgtgct gaccgtggcg gtgatgctgc ccgccagcgg
     8641 ctggctggcg gacaaagtcg gcgtgcgcaa tattttcttt accgccatcg tgctgtttac
     8701 tctcggttca ctgttttgcg cgctttccgg cacgctgaac gaactgttgc tggcacgcgc
     8761 gttacagggc gttggcggtg cgatgatggt gccggtcggc aggttgacgg tgatgaaaat
     8821 cgtaccgcgc gagcaatata tggcggcgat gacctttgtc acgttacccg gtcaggtcgg
     8881 tccgctgctc ggtccggcgc tcggcggtct gctggtagaa tacgcgtcgt ggcactggat
     8941 ctttttgatc aacattccag tggggattat cggtgcgatc gccacattga tgttaatgcc
     9001 gaactacacc atgcagacgc ggcgctttga tctttccggc tttttattgc tggcggttga
     9061 catggcggta ttaaccctgg cgctggacgg cagtaaaggt acaggtttat cgccgctggc
     9121 gattgctggc ctggtcgcag ttggcgtggt ggcactggtg ctttatctgc tgcacgccag
     9181 aaataacaac cgcgccctgt tcagtctgaa actgttccgc actcgtacct tttcgctggg
     9241 cctggcgggg agctttgccg gacgtattgg cagtggcatg ttgcccttta tgacaccggt
     9301 tttcctgcaa attggcctcg gtttctcgcc gtttcatgcc ggactgatga tgatcccgat
     9361 ggtgcttggc agcatgggaa tgaagcgaat tgtggtacag gtggtgaatc gctttggtta
     9421 tcgtcgggta ctggtagcga ccacgctggg tctgtcgctg gtcaccctgt tgtttatgac
     9481 taccgctctg ttgggctggt actacgtttt gccgttcgtc ctgtttttac aagggatggt
     9541 caactcgacc cgtttctcct ccatgaacac cctgacgctg aaagatctcc cggacaatct
     9601 ggcgagcagc ggaaacagcc tgctgtcgat gattatgcaa ttgtcgatga gtatcggcgt
     9661 caccatcgcc gggctgttgc tggggctttt tggttcgcag catgtcagcg tcgacagcgg
     9721 caccacacaa accgtcttta tgtacacctg gcttagcatg gcgtttatca tcgcccttcc
     9781 ggcgttcatc tttgccagag tgccgaacga tacgcatcaa aatgtagcta tttcgcggcg
     9841 aaaaaggagc gcgcaatgaa gttctggcga cccggtatta ccggcaaact gtttctggcg
     9901 attttcgcca cctgcattgt cttgctgatc agtatgcact gggcggtgcg tatcagtttt
     9961 gagcgtggct ttattgatta catcaaacac ggcaatgaac agcgattaca gctgttaagt
    10021 gatgcgcttg gcgagcagta tgcgcagcat ggtaactggc gcttcctgcg caacaacgat
    10081 cgctttgtct ttcagatcct gcgttcattt gaacacgata attcggaaga taaacccggc
    10141 ccgggtatgc cgccgcacgg ctggcgtacc cagttctggg tggtcgatca aaacaacaaa
    10201 gtactggttg gtccgcgagc gccgattcca cctgacggta cacggcgacc cattctggtc
    10261 aacggtgcgg aagtgggcgc ggtgattgcc tcccctgttg agcggctgac ccgcaatact
    10321 gatatcaatt tcgataaaca acagcggcaa accagctggc tgatcgtcgc cctggcaacg
    10381 ttactcgcgg cgctcgccac ctttctgctg gcgcgcggtt tgctggcacc ggtaaaacga
    10441 cttgtcgatg gcacgcacaa actggcggcg ggcgatttca ctacccgcgt aacgcccacc
    10501 agtgaagatg aactgggcaa actggcgcaa gacttcaacc agctcgccag cacgctggag
    10561 aaaaaccagc aaatgcggcg cgattttatg gccgatattt ctcacgaact gcgtacgcca
    10621 ttagcggtgc tgcgcggtga actggaagcc attcaggatg gcgtgcgcaa attcacgccg
    10681 gagacggtgg cgtctttaca ggcggaggtc ggtacactga ccaaactggt tgacgatctt
    10741 catcagttgt cgatgtctga tgaaggcgct ctcgcctatc aaaaagcacc ggtagatttg
    10801 atcccactgc tggaagtggc gggcggcgca tttcgcgaac gattcgccag ccgtggcctg
    10861 aaactgcaat tttccctgcc agacagtatc accgtatttg gcgatcgcga ccgtttaatg
    10921 cagttattca ataacttact ggaaaacagc ctgcgctaca ctgacagcgg cggcagcctg
    10981 aaaatctctg ccgagcagca cgacaaaacg gtgcgcctga cctttgccga cagtgcgcca
    11041 ggtgtcagtg acgatcagct acaaaaattg tttgaacgtt tttatcgcac cgaaggttcc
    11101 cgcaaccgcg ccagcggcgg ttccgggctg gggctggcga tttgcctgaa cattgttgaa
    11161 gcccataatg gccgcattat tgccgcccat tcgccttttg gcggggtaag cattacagta
    11221 gagttaccgc tggaacggga tttacagaga gaagtatgac cgagttacca atcgacgaaa
    11281 acacaccgcg tattttgatc gtggaagatg aaccgaagct ggggcagttg ctcattgatt
    11341 atctgcgtgc tgtgagctat gcgccgacgc ttatcagcca cggcgatcag gtactggcgt
    11401 atgtgcgcca gacaccaccg gatctgatcc tgttggatct gatgctccct ggcaccgatg
    11461 gcctgacatt atgccgggaa attcgtcgtt tttccgatgt tccgatcgtg atggtgacgg
    11521 caaaaatcga agagatcgac cgcctgctgg ggctggagat tggcgccgat gactacatct
    11581 gcaagccgta cagcccacgg gaagtggtag cgcgtgtcaa aaccattttg cgccgttgta
    11641 aaccgcaacg cgaacttcag caacaggatg ccgaaagccc gttgattatc gacgaaggtc
    11701 gttttcaggc ttcatggcgc ggtaaaatgc ttgacctgac gcctgcggaa tttcgtctgc
    11761 ttaaaacgct ctctcacgaa ccaggaaaag tgttctcccg cgagcaattg ctcaatcatc
    11821 tttatgacga ctaccgcgta gtaaccgacc gcaccattga cagccacatt aaaaatctgc
    11881 gccgcaagct ggaatctctc gacgccgaac agtcatttat ccgcgccgtt tacggcgttg
    11941 gttaccgctg ggaagccgac gcctgccgca tcgtttagtt ttagcgacat tactttgtta
    12001 gccggagatg gcgttcagac ccgatccggc aacattattc cacgcatggt cagcaaactg
    12061 acattatctc tccgccctgg caggcatcag aaaatcgctc atactttaat cagtaaacag
    12121 cacctttaga tgctgttttc gataagcaat ttcaatcaag gagtcattat ggctggttgg
    12181 tttgaactca gtaagagcag tgataatcag ttccggtttg tgctaaaagc gggcaatggt
    12241 gagactatcc tcaccagcga gctgtatacc tcaaaagcct ctgcggaaaa gggcatcgcg
    12301 tcggtgcgta gcaacagccc gcaagaagag cgctatgaga aaaaaacggc aagcaacggc
    12361 aaattctatt tcaatctgaa agccgctaat catcaaatta tcggctccag ccagatgtac
    12421 gccaccgcgc aatctcgtga aaccggaatt gcctccgtta aagccaatgg cacaagccag
    12481 acggtgaaag acaatacgta attacgatgc cgggcgtggg ttgttggcgt ccggcgcttt
    12541 accagatccc ccctacagcg ctacaatgcc cgcccttaaa gtgggggcct cccctaaccg
    12601 cttcatcagg tgaagcggat ctgacctgtc atcagaacga gagaattatg tttaaaccgg
    12661 aactcctttc cccggcggga acgctgaaaa atatgcgtta cgctttcgct tatggcgcag
    12721 atgctgttta tgcgggccag ccgcgttact ccctacgtgt gcgcaacaac gaattcaacc
    12781 acgaaaatct tcagctcggc atcaatgaag cccacgcgct ggggaaaaag ttttatgtcg
    12841 tggtcaacat tgcaccacac aacgccaagc tgaaaacctt tatccgtgac ctgaaaccgg
    12901 tggtggaaat ggggccggat gcgctgatta tgtccgatcc agggctgatt atgctggtgc
    12961 gtgagcactt ccctgaaatg ccgatccacc tctcggtgca ggctaacgcc gtgaactggg
    13021 cgacggtgaa attctggcag caaatgggac tgacccgcgt gatcctctct cgcgagctgt
    13081 cgctggaaga gattgaagag atccataatc aggtgccgga tatggagatc gagatcttcg
    13141 ttcacggcgc actgtgcatg gcctactccg gtcgctgcct gctctctggc tatatcaaca
    13201 agcgcgaccc gaaccagggc acctgcacca acgcctgccg ctgggagtac aacgtccagg
    13261 aagggaaaga agatgacgtt ggcaacatcg tacacaagta cgagccgatt ccggtgcaaa
    13321 atgttgagcc gacgctgggt atcggcgcgc caaccgacaa agtgtttatg atcgaagaag
    13381 cccagcgtcc gggcgagtat atgaccgcgt ttgaagatga gcacggcact tacatcatga
    13441 actcgaaaga tctgcgcgcc atcgcccatg tagaacgcct gaccaaaatg ggcgtgcatt
    13501 cgctgaaaat cgaaggtcgt accaaatctt tctactattg cgcacgcacc gcacaggttt
    13561 accgcaaagc tatcgatgac gccgctgcgg gaaaaccgtt cgataccagc ctgctggaaa
    13621 ctctggaagg tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt catactcacg
    13681 acgattatca gaactacgaa tacggttatt cagtttctga ccgccagcag tttgttggtg
    13741 agtttaccgg tgagcgcaag ggggacctcg cagcggtagc ggtgaaaaat aaattctccg
    13801 ttggcgacag tcttgagctg atgacgccgc aaggcaacat taactttacc cttgagcaca
    13861 tggaaaacgc caaaggcgaa gctatgccgg tcgcaccagg cgatggttat actgtgtggc
    13921 tcccggtccc gcaggatctt gagctaaatt acgcgctgct aatgcgtaat ttctccgggg
    13981 aaaccacgcg taacccccac ggtaagtgat taatttcgat tatttttccc ggatggaaaa
    14041 ttcttagaaa ccgatcacat acagctgcat ttattaaggt tatcatccgt ttcgctgaaa
    14101 aacataaccc ataaaatgct agctgtacca ggaaccacct ccttagcctg tgtaatctcc
    14161 cttacacggg cttatttttt gtacataagc tgtaaagaag gattgatccc tgataaccgt
    14221 cttccattga ccacatcgat agaatccccc ttcatagcac aatgcctttc acgtaacggt
    14281 atcgtgctcg cccaggttcc ggctacgcac agccagaacg cgcatgtttg acgcttacca
    14341 aaaaatattc tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac
    14401 cttctgtctt caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg
    14461 catcattttt tactatattt tctgcaccag atattctgcc cctggctaaa gaagcttcgg
    14521 cttcggtgta gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa
    14581 atttaccgat acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa
    14641 ttaatgatat agcagctatc tttttcatct caccttcccc cattaaatac caacgacact
    14701 ctctagtgtt taaatataat aatggcatga ttattataat tgaataggat tataataaat
    14761 gttctgtaca acatttccta cataagtagg aattacggac attgaggcac ttcagggtaa
    14821 ctccatgggg gctttaatat attatattga agatgccact gtttagttga atattaggta
    14881 tatgctcttt tttgaaattt atcggtggca gccgttagta ttcgctgtcc ccattgcaag
    14941 ctcctggtgg taaccactga atcctccata cttgaactga ctttttatcc tccgactttc
    15001 atcctgttct gactccacct tttgttttct gctctacact atctacagac caatcataaa
    15061 ggcacatacg atcatggcag aatttcccgc cagcttactg attcttaatg gcaaaagtac
    15121 tgacaatcta cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa tgacgatcca
    15181 tgtgcgggtc acctgggaga aaggtgatgc cgcacgattt gtagaggagg cccggaagtt
    15241 gggcgtcgca acggtgattg ccggtggtgg cgatggcacc attaatgaag tttctacggc
    15301 gttgattcag tgtgaggggg atgacatacc cgcgctggga attttgccat taggaaccgc
    15361 caatgatttt gccaccagtg tagggattcc tgaggcactg gataaggcgc tgaaactggc
    15421 aattgccggt aatgccatta cgatagatat ggcgcaggtc aacaaacaaa cctgttttat
    15481 taatatgccg acaggcggat ttgggacgcg tattaccaca gaaacgccgg aaaaattaaa
    15541 agccgcgctg ggtggcgtct cttacatcat tcatggctta atgcgcatgg atactctgca
    15601 accggaccgt tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg ccctggtcat
    15661 tggtattggt aacgggcgtc aggccggtgg cggtcaacaa ttgtgcccga acgcgttaat
    15721 taacgatggc ttgctgcaac tgcgcatttt taccggcgat gaaattcttc cggctctcgt
    15781 atcaacctta aaatctgacg aagataaccc gaatattatc gaaggcgctt cgtcgtggtt
    15841 tgatatacaa gccccacacg aaatcacttt taatcttgat ggcgaaccgt tgagtgggca
    15901 aaacttccat attgaaatac ttccggcggc gttgcgttgt cgattaccac cagattgtcc
    15961 attattgcgt taatcaaact attctctctt gaaccaggga gaatccgtaa tgccggatgc
    16021 ggtgtaaaca ccttatccgg catacagaac aatactacac cgtaattaat tttacccgtg
    16081 tcttctgcaa tgccaattta tccacctctg acacagcatc atcggtaaca ataatgtcaa
    16141 ttttttcgat tggcagtacc tgattaaaac cgcgacgatt aaatttcgat gaatcgagaa
    16201 cggcaatcac tttatttgcg gcggtgacca tcgccccgct aatcgaataa ccttcattaa
    16261 aggtcgtaat accattaacc gcatcaatac catcagcacc gacaaacatt aaatcggcat
    16321 taatatcctg caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt ttatggcgga
    16381 ccgtgccacc acagacgacc agagtaatgt ctttattttc ggaaagggca aacgctgccg
    16441 ggagactgtt ggtaatcacc gtgatatttt tagcggtcat taatccttcg gcgataagca
    16501 ttgtggtact tccgctgtcg agaataacgg tcatcccttc atggatcatt ttgactgccg
    16561 cctgcgctat tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta aacccgactt
    16621 cctgggtctc ggtttcacta ttaccggaca ttattttcgc cgcaccgcca tgaaagcgcg
    16681 taaccacgcc tttttgttcg agaaagcgca aatcggcacg gattgtcgct tccgaggcag
    16741 caaatactcc cgccagatcc tgaacaagca cggttccctg ttcattcact aattggatga
    16801 tcttattcct tcgctcgaat gaattcatcg tcatttaccc atattcaatt gtggctagtg
    16861 taaacgaagg gggaccattg gtgaatgatc tgttttgcac aataccgggc cagcatcacg
    16921 ctggcccgcg gtttcatgga atgagcaaca ctttgcccgg catggcatta cgagcgatgt
    16981 cacgcaccgc ctgggtgaag ctttcaaagc ttccacggtg agcgattaat ggatccaggc
    17041 ttaacttacg ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc ggccaagggc
    17101 tggaatagtt catccaactg ccgataaccg tcagctcttt acgcaatatt ttgccaaacg
    17161 ttgccgatgt taaatgcaga tcctgatgca acgtgcccac cagcgccagt tgggcatgag
    17221 gaccggcaat ctctaccgcc agttcgacag tttgcggtac gccagccgtc tcgaggataa
    17281 gctgattaaa gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc atttcgctac
    17341 tgttaaatgt ttgcatcgca ccgaaagatt ttgccagtgc cagtttttct gagctaatgt
    17401 cgatcgccgt cacactcttt gctcccagcg cgaca
//
LOCUS       DBKEKI010000074        17426 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.074, whole genome shotgun sequence.
ACCESSION   DBKEKI010000074 DBKEKI010000000
VERSION     DBKEKI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.074"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..91)
                     /locus_tag="ACUQPX_002539"
     CDS             complement(<1..91)
                     /locus_tag="ACUQPX_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057221.1"
                     /translation="MEFSFSPKRLVVAVAAALPLMASAADTPST"
     gene            complement(770..1966)
                     /gene="hemY"
                     /locus_tag="ACUQPX_002540"
     CDS             complement(770..1966)
                     /gene="hemY"
                     /locus_tag="ACUQPX_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HHV9057222.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1969..3216)
                     /gene="hemX"
                     /locus_tag="ACUQPX_002541"
     CDS             complement(1969..3216)
                     /gene="hemX"
                     /locus_tag="ACUQPX_002541"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HHV9057223.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPAPQGDTPAAAPQGE"
     gene            complement(3238..3978)
                     /gene="hemD"
                     /locus_tag="ACUQPX_002542"
     CDS             complement(3238..3978)
                     /gene="hemD"
                     /locus_tag="ACUQPX_002542"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HHV9057224.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3975..4916)
                     /gene="hemC"
                     /locus_tag="ACUQPX_002543"
     CDS             complement(3975..4916)
                     /gene="hemC"
                     /locus_tag="ACUQPX_002543"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HHV9057225.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5303..7849
                     /gene="cyaA"
                     /locus_tag="ACUQPX_002544"
     CDS             5303..7849
                     /gene="cyaA"
                     /locus_tag="ACUQPX_002544"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HHV9057226.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7889..8209)
                     /gene="cyaY"
                     /locus_tag="ACUQPX_002545"
     CDS             complement(7889..8209)
                     /gene="cyaY"
                     /locus_tag="ACUQPX_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HHV9057227.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8257..9570)
                     /locus_tag="ACUQPX_002546"
     CDS             complement(8257..9570)
                     /locus_tag="ACUQPX_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HHV9057228.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9567..11032)
                     /locus_tag="ACUQPX_002547"
                     /pseudo
     CDS             complement(9567..11032)
                     /locus_tag="ACUQPX_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            11221..11424
                     /gene="lptM"
                     /locus_tag="ACUQPX_002548"
                     /gene_synonym="yifL"
     CDS             11221..11424
                     /gene="lptM"
                     /locus_tag="ACUQPX_002548"
                     /gene_synonym="yifL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS translocon maturation chaperone LptM"
                     /protein_id="HHV9057229.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11461..12285
                     /gene="dapF"
                     /locus_tag="ACUQPX_002549"
     CDS             11461..12285
                     /gene="dapF"
                     /locus_tag="ACUQPX_002549"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HHV9057230.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12282..12989
                     /locus_tag="ACUQPX_002550"
     CDS             12282..12989
                     /locus_tag="ACUQPX_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HHV9057231.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLVRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12986..13882
                     /gene="xerC"
                     /locus_tag="ACUQPX_002551"
     CDS             12986..13882
                     /gene="xerC"
                     /locus_tag="ACUQPX_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HHV9057232.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     QQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13882..14598
                     /gene="yigB"
                     /locus_tag="ACUQPX_002552"
     CDS             13882..14598
                     /gene="yigB"
                     /locus_tag="ACUQPX_002552"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HHV9057233.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14682..16844
                     /gene="uvrD"
                     /locus_tag="ACUQPX_002553"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14682..16844
                     /gene="uvrD"
                     /locus_tag="ACUQPX_002553"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HHV9057234.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16991..>17426)
                     /locus_tag="ACUQPX_002554"
     CDS             complement(16991..>17426)
                     /locus_tag="ACUQPX_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HHV9057235.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQF
                     AVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         4055 a         4491 c         4627 g         4253 t
ORIGIN      
        1 cagttgacgg ggtatctgct gcgctggcca tgagaggaag agcggcggcg acagcaacaa
       61 caagacgttt gggtgaaaac gaaaattcca tgcaaaatgc tccggtttca tgtcatcaaa
      121 atgatgacgt aattaagcat tgataattga gatccctctc cctgacagga tgattacata
      181 aataatagtg acaaaaataa attatttatt tatccagaaa atgaattgga aaatcaggag
      241 agcgttttca atcctacctc tggcgcagtt gatatgtaag gcaggtttat tatatcgcgt
      301 tgattattga tgctgttttt agttttaacg gcaattaata tatgtgttat taattgaatg
      361 aattttatca ttcataataa gtatgtgtag gatcaagctc aggttaaata ttcactcagg
      421 aagttattac tcaggaagca aagaggatta cagaattatc tcataacaag tgttaaggga
      481 tgttatttcc cgattctctg tggcataata aacgagtaga tgctcattcc atctcttatg
      541 ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt gcttatcgtc
      601 cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg agtgaagcac
      661 ccacttgttg tcatacagac ctgttttaat gcctgctccg taataagagc aggcgttttt
      721 ttatgcatca gaaaggtccc ggaggtgctt tcctccgggt gagaaggaac tactgtggcg
      781 ggttattctg caacgttaac atcaaaccgt cgcgacgcat cgctgcagct tcttccggct
      841 tgtgcagtct gtccagcgcg tcggcaagcc atgcgtaatc gtaggcgtcc ggacgttgtt
      901 tcagcgccgc gcggaaggcg agcgatgctt cctgccattc accgtgcttc atcagtgact
      961 gacccaatgt gctccacaac agcgggcgat caccgacgtt tttgatttgc tggcgtagca
     1021 ctttttccag ctgttccggg ttattggttt tcagacgcgg gatcggcagc agtaggcgat
     1081 cgtcatactg gcgtttcagg ccgtcgatga taatttgctg ggcagtatca tgatcgtcac
     1141 attcaataag atgttccgcc attgccacct gcaacgctac ctgatgacgc gttttccggc
     1201 tttggttttt ccaccagtta cgcaaacctt cactaccgtt atcggcacgc gcctgatcca
     1261 tcaggccaat ccatgcctgt tgttccagca ttgcacgatg ttcttcatca ccaacatggg
     1321 ctttcgccat tgatgggatg atatccagca gagaactcca tgcccctgtg cggatatacg
     1381 cctgttccgc cagacgtaat acttcaggat ggcgtggcgt aacttccagc agcttatcca
     1441 cgccgtggcg tgcagcatgg ttttcattac gggccagttg cagacgtaca cgggtgattt
     1501 ctaccggaat ggtgtcattg ccggccagct ccgctgcgcg ttccagatgt tggttggcgc
     1561 gtgcttcatc accacgttgt tgcgcggctt cggcagccag tagatagttc accaccggtt
     1621 gttcagcgtg atcggcattt ttcgccatca gcttttcaac ttgctgataa tcaccttccg
     1681 ccagtttcag cagcgcctgt tcggtctgct tacgcgcacg gcgacgctta cgtccgacaa
     1741 accatccacg ggtgtgcgcg ccagtgcgga agatccgccg cagtagccac tcaatggcaa
     1801 acagcactac catcgccaga atcaatatga tcgccaggcc cgtgacgctg gtttcgatat
     1861 tgtagttgtc ggtctggatc agcacataac cctgatgacc ggcaatcatc gggccaacca
     1921 ctatccccgc aatcagcaaa acaaagagta ataacacttt tagcatggtt attctccttg
     1981 cggcgcggct gccggagtat caccttgcgg tgcaggtgca ggtgcaggtg caggtgcagg
     2041 tgcaggtgca ggtgcaggtg caggtgcagg tgcaggtgca ggtgcaggtt tagcttccgt
     2101 tgtccccgct gccggttgtg ccagcaggtt acgcacgcga gtctgcatca gtttttccag
     2161 catcgcctgg ctttgcaggg tttccggaag atccatcgag atattttgct ggcttaactg
     2221 gtccacctcg tcgaggaacg ctttggtggt ggcatcatca gtatcgtagt aagcacgtac
     2281 ccaggtggag acgttctcca gcgcctggcg ataagtctct tcctgatgac gcggtacagc
     2341 ttgtgctgcg accagcaggc gagagcgaat attttcgcgc agatagatat cctgatttgg
     2401 cgctaacagc ggtacggcgg tgtcatcacg acggcgaatc gtaatgaagt tgtccataaa
     2461 gttctgccag cttttttgca gattgatacg ccattcgctg atggaactgg aaagctcttc
     2521 accgtctgaa tccatcggcg aaccatcgct gtcattatcg gccagacgca ggttatctac
     2581 ctgatttgaa agctgattaa gcttaaggat gatgccgtca taatccacct gcgatactgc
     2641 agaaaggctg gcgatatcat cggtaattgc ccgacgaacg gtaatcagac tcgggtcatt
     2701 catatccgcc aggctggcgt ctgcactttt cagcaacgct gcagaggtcg tgacgtcctg
     2761 atcgctccac agcttccgtc cggcgagttt caccagaaaa tcggcctgag ccagcagcca
     2821 ggttttagca tcgctgccgg aaatggtggc gatcttttgt tggacttcat ccaactgttt
     2881 tgccagcgtt tcttgctgac gattcgcctg cttaagctgc gcagcttgtt gcttaataat
     2941 gccttccagc tcggcttttt ggctctcctg ggctttttgc aatgccgtca gttggttagc
     3001 cagggcatcg ctggtggcgg tctgattgac ggcctgttgt ttaccccagc catacaaacc
     3061 gacacccgcc gccagagcaa tagcgatagc caccgcgctg agaatcaatg cagtattgtt
     3121 cttactcttt ttttctgttg cgacaggttg tgacgtggtg tccacggcct ccctagtctc
     3181 ttcaaccacg gcggaggttt tttcttgttc cgtcattatg gcttcctgtt atgagagtta
     3241 ttgtaatgcc cgtaaaagcg catcgttgtc agcgttatcg gcgaccttaa tgtcttgcca
     3301 gcccagttcc cgggcgagtt tcgccaaacg ctcactgacg accaatagtc gacagtgtag
     3361 taaccagtgc tcacgatacc attgtgggat cagcgaccag agttgctgca acatttcacc
     3421 gctggtaaca acgaccatcg tcacctcgcg ggattgccag cgcatcgctt cttctgcacc
     3481 atcgtaatgg attgcgcatc gttgataaca ttcacaaaaa gtgacctcag caccgcgcac
     3541 cgtcagggta tccccaatta gctcacgacc gccattgcca cgtaatatca gcgcacgttt
     3601 gcccgcaata ttttgtaatt caggtaattg tagcaagact tcgctgattt cccgatcctg
     3661 tgggtagaga atcttctgtc cgcttacggt atgtagcgcc agtgcggtgg tgcgtccaat
     3721 ggcgaaataa gtaggtagtt ggggccattt acgatcttgc tgatgcagct gtgattgggc
     3781 aaaagcaacc gcgtgttgcg agagggcaaa caacagatcg ctctccccca gcgctgccag
     3841 ttgatcagca agttgcggta attgtcgacc cggagaaaac tcaatcagcg gaaaatgcca
     3901 ggccacctgc cccagtgtgc gcagacggct cactaactct tctccagcgg gagacgggcg
     3961 ggtgaccagg atactcatgc cggggcctct ccgttataga cttcagcgag gatctcgcgc
     4021 gcgccgttat tcagtagctc ttctgccagc gaaatcccca tttgttcggc atcttgcggc
     4081 gcaccgcggc gttcaccgcg aataatctgc gaaccgtccg gcgcgccgac cagcgcacgt
     4141 agccagattt cgccatcaat aagctcggcg tagctaccaa ttggcacctg acatccgcct
     4201 tcgagacggg tattcatggc gcgttctgcg gtaacgcgca gtgcagtttc gtggtgattc
     4261 agcgcggcaa gcagctcgcg agtgcgtgta tcatcaaggc ggcattcaat accgactgca
     4321 ccttgtccta ccgccggaag agaaatctcg ggtggtaacg cggcgcgaat gcgggactcc
     4381 agacctaaac gttttagtcc ggctacggca agaatgatgg catcgtattc gccgttatct
     4441 agtttgctca ggcgagtgcc gacgttgccg cgcagggagc ggataatcag atccgggcgg
     4501 cgttcagcca gttggcactg gcgacgtaaa ctggacgtcc cgacgatact gcctgctggt
     4561 aacgcatcca gactgtcata gttattggac acaaaggcat cgcgaggatc ttcacgctcg
     4621 caaatagtga ccagccccag accttgcggg aattcaaccg gcacatcttt cattgaatgt
     4681 acggcgatat cagcgcgatt ctccaggagc gcgacttcca gctcttttac aaataagcct
     4741 tttccgccta ctttcgccag cggcgtatca agaatcacat cgccgcgcgt caccatcggt
     4801 accagttcaa cgaccaggcc cggatggctc gccatcaact tgtctttgac atagtgtgcc
     4861 tgccagagtg caagtgggct ttggcgtgtg gcaattctta aaacattgtc taacatgctt
     4921 gttaccgtca ttatcatccg tggtccatcc taacatcctt gccagagtga tgtcagtgtt
     4981 gtggtgaaac gtagacgcct gcgcaaaccg taaaatgagg tctggcagtg gatcctgaca
     5041 ggcgtttcac gccgtcgtaa taaggaattt acagagaata aacggtgcta cacttgtatg
     5101 tagcgcatct ttctttacgg tcaatcagca aggtgttaaa ttgatcacgt tttagaccat
     5161 tttttcgtcg tgaaactaaa aaaaccaggc gcgaaaagtg gtaacggtta cctttgacat
     5221 acgaaatatc ccgaatgccg cgtgttaccg ttgatgttgg cggaatcaca gtcatgacgg
     5281 gtagcaaatc aggcgatacg tcttgtacct ctatattgag actctgaaac agagactgga
     5341 tgccataaat caattgcgtg tggatcgcgc gcttgctgct atggggcctg cattccaaca
     5401 ggtctacagt ctactgccga cattgttgca ctatcaccat ccgctaatgc cgggttacct
     5461 tgatggtaac gttcccaaag gcatttgcct ttacacgcct gatgaaactc aacgccacta
     5521 cctgaacgag cttgaactgt atcgtggaat gtcagtacag gatccaccga aaggtgagct
     5581 tccaattact ggtgtataca ccatgggcag cacctcgtcc gtagggcaaa gttgttcctc
     5641 tgacctggat atctgggtct gtcatcaatc ctggctcgat agcgaagagc gccagttact
     5701 acaacgtaaa tgcagcctgc tggaaagctg ggccgcctcg ctgggtgtag aagtcagctt
     5761 cttcctgatt gatgaaaacc gcttccgtca taatgaaagc ggcagcctgg ggggcgaaga
     5821 ttgtggctcc acccagcata tactgctgct tgacgaattt tatcgtactg ccgtgcgtct
     5881 cgccggtaag cgtattctgt ggaatatggt gccgtgcgac gaagaagagc attacgacga
     5941 ctatgtgatg acgctttacg cgcagggcgt gctgacgcca aatgaatggc tggatctcgg
     6001 tggcttaagc tcgctttctg ctgaagagta ctttggtgcc agcctttggc agctctacaa
     6061 gagtatcgat tccccataca aagcggtgct gaaaacactg ctgctggaag cctattcctg
     6121 ggaatacccg aacccacgtc tgctggcgaa agatatcaaa cagcgtttgc acgacggcga
     6181 gattgtatcg tttggcctcg atccatactg catgatgctg gagcgtgtta ctgaatacct
     6241 gacggcgatt gaagatttca cccgtctgga tttagtacgt cgctgcttct atttaaaagt
     6301 gtgcgaaaaa ctcagccgtg aacgcgcctg tgtaggctgg cgtcgcgcag tgttgagcca
     6361 gttagtgagc gagtggggtt gggacgaagc tcgtctggca atgctcgata accgtgctaa
     6421 ctggaagatt gatcaggtgc gtgaggcgca caacgagctg ctcgacgcga tgatgcagag
     6481 ctaccgtaat ctgatccgct ttgcgcgtcg caataacctt agcgtctccg ccagtccgca
     6541 ggatatcggc gtactgacgc gtaaactgta tgccgcgttt gaagcattgc caggtaaagt
     6601 gacgctggta aacccgcaga tttcacccga tctctcggaa ccgaatctga cctttattta
     6661 tgtgccgccg ggccgggcta accgttcagg ttggtatctg tataaccgcg cgccaaatat
     6721 tgagtcgatc atcagccatc agccgctgga atataaccgt tacctgaata aactggtggc
     6781 gtgggcatgg tttaacggcc tgctgacctc gcgcacccgt ttgtatatta aaggtaacgg
     6841 cattgtcgat ttgcctaagt tgcaggagat ggtcgccgac gtgtcgcacc atttcccgct
     6901 gcgcttacct gcaccgacac cgaaggcgct ctacagcccg tgtgagattc gccatctggc
     6961 gattatcgtt aacctggaat atgacccgac agctgcgttc cgcaatcagg tggtgcattt
     7021 tgatttccgt aagctggatg tcttcagctt tggcgagaat caaaattgcc tggtaggtag
     7081 cgttgacctg ctgtaccgca actcgtggaa cgaagtgcgt acgctgcact tcaacggcga
     7141 gcaatcgatg atcgaagccc tgaaaactat tctcggcaaa atgcatcagg acgccgcacc
     7201 gccagatagc gtggaagtct tctgttatag ccagcatctg cgcggcttaa tccgtactcg
     7261 cgtgcagcaa ctggtttctg agtgtattga actgcgcctt tccagcaccc gccaggaaac
     7321 cgggcgtttc aaggcgctgc gcgtttctgg tcaaacctgg ggcttgttct tcgaacgcct
     7381 gaatgtatcg gtacagaagc tggagaacgc catcgagttt tatggcgcta tttcgcataa
     7441 caaactgcac ggcttgtcag tgcaggttga aaccaatcac gtcaaattac cggcggtggt
     7501 ggacggcttt gccagcgaag ggatcatcca gttctttttc gaagaaacgc aagacgagaa
     7561 tggctttaat atctacattc tcgacgaaag caaccgggtt gaggtgtatc accactgcga
     7621 aggcagcaaa gaggagctgg tacgtgacgt cagtcgcttc tactcgtcat cgcacgaccg
     7681 cttcacctac ggctcaagct tcatcaactt caacctgccg cagttctatc agattgtgaa
     7741 ggttgatggt cgcgaacagg tgattccgtt ccgaacaaaa tcgattggca acctgccgcc
     7801 cgccaatcag gatcacgata cgccgctatt acagcagtat ttttcgtgat gagcgtgccg
     7861 gaaagcgagg cttatccggc atgcaatctt agcggaaact gactgtttca cccgcctgtt
     7921 gcgtcgccgc ctgttccagc aaatcccaga aggtttcgcc gctgcgatcg caaatccatt
     7981 catcgccttt caggtcaaaa tggtagccgc cctgtttggt tgccagccac acctggtgca
     8041 gcggctcctg gcggttaata atgattttgc tgccgttttc aaaggtaatg gtcagtacgc
     8101 cgccgttgat ttcgcagtcg atatcgctgt cgccatccct gtcgtccaga cgctcttcaa
     8161 tggtcagcca cagttgatca gccaggcgat gaaattcact gtcgttcatt gttgtatcct
     8221 gtttttaagt gatggcggca gtatagcggc atggggtcag ggcttcaaag tttgcacctc
     8281 tgctgctgcg ttccggcatg attcatccgt caccggaata atgatgtctc tgttttgaac
     8341 ttcacgtacg ttctcctctc tgacacgagt tttcgccggg cagggggagg gcgtaatcct
     8401 tacctctgta tcggtccatg tataaacatt gcgatcgtca taagcgaagc gatcgttttt
     8461 agttagcgga ccgttaactg gaataatttg caagctcttt gaccgtaatt ggcggataaa
     8521 gtaagcatgc tgtttgccgc tattaaatga gcgatcaatg accaggtctg gcgtatccag
     8581 cgggattatc tcaagctggt cttcaccaaa tgtcgataat tttgttacat acagccgatc
     8641 tttatattgc gcaatattgc cagtaatcag atggaattgt tcaggttcaa tacctggtag
     8701 gtctatccac ttgctccact gacggtcggg cgtgagctgt ttacgccagc gaacgcgact
     8761 ttcccctact tcaaaagaga cgtcatcatc tacgggtatc gctttgcctg ataattgacc
     8821 aaagggataa cgatgcagac ccttattgtc gcgaaaaacc agcagtgaat gaggtatcca
     8881 gcgaattatc tcaaatgagt ctgcattcgc cgtaaggtga acgccattta aataaatatt
     8941 ctgcccactg cggataagca gatcattgga atatgaattt ctgtttacac tcataagacg
     9001 tggattaata ccccaccatc ttttttctaa tacagtaaca tcactgctgc tcccctgacg
     9061 tcgaccggac aagtagagat ttttactatc caccagcgtg gtagagttac cttcgatagc
     9121 ttttagtgtc gccagatcaa cacgtgacgc gccgctatta ctttcggtgc gctgtccgtc
     9181 aaaatagagg ctatatttat cgaccgcgaa gcggcccata gcctgaaagc tggcaatatc
     9241 gacagtgggt gttcccggtg gattgctaac gattttgcca cgccagagaa taacgcggcc
     9301 gtctgtcacc cagtggtatt cctcattttc tatgcttatt tcatcggaag gtatataacc
     9361 cgttttccca gagatatttt tcacaaggcg caaatcggcc ctgctcatct ccggaaactg
     9421 ggcaatgatt ttcttatccc cccaataacc tttaccgtaa gacataacac cgtgatcatt
     9481 gtaatagtcg aaccctatgg cctgtacagc aggcgtcaga aatgtgtgtc ccaacaggat
     9541 gagtgcaact gtgattattt gacccttcat tttgtcgcct cattgcgcgg ggcaggaata
     9601 aattcgccgt catcagtaat ttttccgtcc agatatcccg ttttgtaatt tacgccatag
     9661 ccatatcccg aatgttgttc cttccagatt aagcgaactt gtttcggatc agcggtgcgg
     9721 tatcgccaga gctgcgcgcc atcaaagctg taaatgtagc ggttatcgcg ggcataccat
     9781 ctgcgcaaaa agggatggtc attaagatgg gcttcgcgtt catccggaga acggttatcc
     9841 agtaagatca gcggtccgtc agattcaaac acgtgtaaac cggaatggta tggccactct
     9901 tcggcacgcg taagtaaata gccgtggcgt ttcccggcgt tgaaacgttt gttgataata
     9961 agattgggat catcaagttt cacgatctcc agcttacggt caccagactc tgtttcgatg
    10021 atggtataaa gcgagtcctg atactgggcg acagcatcac tgatgggttg aaactgctcc
    10081 ggatcgagtc cgggtaattc ttcctgttta caagcaggtc ctttacgcca gaacactttc
    10141 ttttccagca gagaaaaatc cagacagtgc tttgttaaat cttcatccca cgccagattt
    10201 tccttgtcga ggactttacg ctgcagcccg tttttatcac gccaggtcaa gaatgagccg
    10261 ggcatccagc gcacgacgga aaaggtgtcc gggtcggcat caagctgttc gccgttgagc
    10321 aggattttgg tccgtgtacg agccagggta tcccaggggc caaatggtac ggcaatgcag
    10381 gggttaaacg tggtggaaaa ctttcctcgc tgatcccagg acttctgcgc cagcacacgg
    10441 aaactctcgg ggtcagcgag gcgatgccca ttgatataca agaaattagc gtcacgtaat
    10501 atcagcccca gtaagtcagg atcccaggga ggccggaatt ccacctgatg cagcgttttg
    10561 atatccaagc tatttccacc accattgtca tcggtgcgct tgccttcaaa atagagactg
    10621 tgtttatctg cggcaaaatc accccatgcc ttgaacgagg cgacatcgac gggaggtgtg
    10681 ccgggagggt tttgcacaac ttcacccgcg tagaggatat tgcgaccatc gctatgccat
    10741 gaataatgag gataccaggc ctgtgcgccc tcatagaggt agtcggttgt caaatcagtc
    10801 agaggcggtt ctttatccca ttcgtctagt gttgtcgggt ctattaataa attgggtaac
    10861 cgacgcaaac tcttgtaatt cacgtcaggt agagaaagat ttgtttcttt ataagggcgc
    10921 aggtacacca cgccttttcc ctgtttctgg taaatattat gagccgacat ccccatgccg
    10981 acgataaaaa caatgaacaa gccaacaaac aaaccacatt gcgatagtgc ataaagccat
    11041 cctggcgcga ggtgttgatc acgaaactgc cagcagaaca taaatcctca cgagtaagcg
    11101 ttatactcgc agcatttcct cacttttcag acttcataaa gagtcgctaa acgcttgctt
    11161 ttacgccttc tcctgcgatg atagaaagca gaaagcgatg aactttacag gcaatccata
    11221 atgaaaaacg tgtttaaggc actcactgta ttacttactc tcttcagcct gacgggctgc
    11281 ggtctgaaag gtccgctcta tttcccgcct gcagataaaa acgcaccgcc gccgaccaaa
    11341 ccggtagaga cgcaaacgca atccacggtg ccggataaaa acgatcgcgc cactggcgat
    11401 ggtccatccc aggtgaatta ctaaaagtca gtttctgtac ccgcgtgatt ggagtaaatg
    11461 atgcagttct cgaaaatgca tggccttggc aacgatttta tggtcgtcga cgcggtaacg
    11521 cagaatgtct ttttttcacc ggagctgatt cgtcgcctgg cggatcggca cctgggggta
    11581 gggtttgacc agctgctggt ggttgagccg ccgtatgatc ctgaactgga ttttcactat
    11641 cgcattttca atgctgatgg cagtgaagtg gcgcagtgcg gtaacggtgc gcgctgcttt
    11701 gcccgttttg tgcgtctgaa aggactgacc aataagcgtg atatccgcgt cagcaccgcc
    11761 aacgggcgga tggttctgac cgtcaccgat gatgatctgg tccgcgtaaa tatgggcgaa
    11821 cccaacttcg aaccttccgc cgtgccgttt cgcgctaaca aagcggaaaa gacctatatt
    11881 atgcgcgccg ccgagcagac aatcttatgc ggcgtggtgt cgatgggaaa tccgcattgc
    11941 gtgattcagg tcgatgatgt cgataccgcg gcggtagaaa cgcttggtcc tgttctggaa
    12001 agtcacgagc gttttccgga gcgcgccaat atcggtttta tgcaagtggt taagcgcgag
    12061 catattcgtt tacgcgttta tgagcgtggg gcaggagaaa cccaggcctg cggcagcggc
    12121 gcgtgtgcgg cggtggcggt agggattcag caaggtttgc tggacgaaga agtacgcgtg
    12181 gaactccccg gcggtcgtct tgatatcgcc tggaaaggtc cgggtcaccc gttatatatg
    12241 actggcccgg cggtacatgt ctacgacgga tttattcatc tatgaagcaa ccaggggaag
    12301 aactgcagga aacactcacg gagcttgatg accgggcggt tgtcgattat ctgattaaaa
    12361 atcctgagtt ttttatccgc aatgcgcgcg cagtggaagc gatacgtgtg ccgcatccgg
    12421 tacgcggcac cgtttcgttg gtcgagtggc acatggcccg cgcacgtaat cacattcatg
    12481 ttctggaaga gaacatggcg ttgttgatgg agcaggctat cgccaacgaa gggctgtttt
    12541 atcgcctact ctacctgcag cgcagtctca ccgccgccag cagtctcgac gatatgctgg
    12601 tgcgctttca ccgctgggcg cgcgatctcg gcctggctgg tgcgagtctg cgcctgtttc
    12661 cagatcgctg gcgcttaggt gcgccgtcga accacactca tctggcatta agccgtcagt
    12721 ctttcgaacc gctgcgtatt cagcgtttgg ggcaggaaca gcactatctt gggccgctta
    12781 acggaccgga gctgctggtg gtgctaccgg aagcgaaagc ggtgggatcg gtggcgatgt
    12841 cgatgctggg aagcgatgct gatttgggtg ttgtgctgtt tactagtcgc gatgccagtc
    12901 actatcaaca agggcaggga acgcagttac ttcatgaaat tgcgctgatg ttgccggagc
    12961 ttctggagcg ttggattgaa cgcgtatgac cgatttacac accgatgtag aacgctacct
    13021 acgttatctg agcgtggagc gtcagcttag cccgataacc ctacttaact accagcgtca
    13081 gcttgaggcg atcatcaatt ttgccagcga aaacggccag caaagctggc aacaatgcga
    13141 tgcagcgatg gtacgcaatt ttgctgtacg cagtcgccgt aaagggctgg gagcagcaag
    13201 tctggcgtta cggctttctg cgctacgtag cttttttgac tggctggtca gccagaacga
    13261 actcaaagct aacccggcga aaggagtttc ggcaccgaaa gcgccgcgtc atctgccgaa
    13321 aaatatcgac gtcgacgata tgaatcggct gctggatatt gatatcaacg atcccctcgc
    13381 tgtacgcgac cgtgcaatgc tggaagtgat gtacggcgcg ggtctgcgtc tttccgagct
    13441 ggtggggctg gatatcaaac acctcgacct ggagtccggc gaagtgtggg tgatggggaa
    13501 aggcagcaaa gagcgccgcc tgccgattgg tcgcaacgct gtggcgtgga ttgagcactg
    13561 gcttgatttg cgcgacctgt ttggtagcga agacgacgcg ctttttctgt cgaaactggg
    13621 caagcgtatc tccgcgcgta atgtgcagaa acgctttgcc gaatggggca taaaacaagg
    13681 gctgaataat cacgttcacc cacataaatt acgtcactcg ttcgctacgc atatgctgga
    13741 gtcgagcggc gatcttcgtg gtgtgcagga gctactgggt catgccaacc tctccaccac
    13801 gcaaatctat actcatcttg attttcaaca ccttgcctcg gtgtacgatg cggcgcatcc
    13861 acgcgccaaa cgggggaaat aatgcgtttt taccggcctt tggggcgcat ctcggcgctc
    13921 acctttgacc tggatgatac cctttacgat aaccgtccgg tgattttgcg caccgagcga
    13981 gaggcgctta cctttgtgca aaattatcat ccggcgctgc gcagcttcca gaatgaagat
    14041 ctgcaacgcc tgcgccaggc ggtacgggaa gcggaacccg agatttatca cgacgtgacg
    14101 cgttggcgtt ttcgttcgat tgaacaagcg atgctcgacg ccgggctgag tgccgaagaa
    14161 gccagtgcag gcgcacacgc agcaatgatt aactttgcca aatggcgcag ccgaatcgac
    14221 gtcccgcagc aaactcacga caccttaaaa cagctggcga agaaatggcc gctggtggcg
    14281 atcaccaacg gtaacgccca gccggaactg tttggtttgg gggattattt tgagtttgtg
    14341 ctgcgcgctg gcccgcacgg gcgctcaaaa ccgttcagcg atatgtactt tttggctgcg
    14401 gaaaaactca acgtgccgat cggcgagatc ttacatgttg gggacgatct caccactgac
    14461 gtgggtgggg caattcgcag cggaatgcag gcttgttgga tcagaccgga aaatggcgat
    14521 ctgatgcaaa cctgggacag ccgtttactg ccacatctgg aaatttcccg gttggcatct
    14581 ctgacctcgc tgatataatc agcaaatctg tatatatacc cagctttttg gcggagggcg
    14641 ttgcgcttct ccgcccaacc tatttttacg cggcggtgcc aatggacgtt tcttacctgc
    14701 tcgacagcct taatgacaaa cagcgcgaag cggtggccgc gccacgcagc aaccttctgg
    14761 tgctggcggg cgcgggcagt ggtaagacgc gcgtactggt gcatcgtatc gcctggctga
    14821 tgagcgtgga aaactgctcg ccatactcga ttatggctgt gacgtttacc aacaaagcgg
    14881 cggcggagat gcgtcatcgt atcggacaac tgatgggcac cagccagggc ggcatgtggg
    14941 taggcacctt ccacgggctg gcgcaccgcc tgctgcgtgc gcaccatatg gacgccaatc
    15001 tgccgcagga tttccagatc ctcgatagcg aagaccagct acgcctgctt aagcgtctga
    15061 tcaaagccat gaacctcgac gagaagcagt ggccgccgcg ccaggcaatg tggtacatca
    15121 acagccagaa agatgaaggc ctgcgtccgc atcatattca aagctacggt aatccggtgg
    15181 agcagacctg gcagaaggtg tatcaggcgt atcaggaagc gtgcgatcgc gcgggcctgg
    15241 tggacttcgc cgagctgctg ctgcgcgctc acgaactgtg gcttaacaag ccgcatatcc
    15301 tacaacacta ccgcgaacgt tttaccaata tcctggtgga cgaattccag gataccaaca
    15361 acattcagta cgcgtggatc cgcctgctgg cgggcgacac cggcaaagtg atgatcgtcg
    15421 gtgatgacga ccagtcaatc tacggctggc gcggggcgca ggtggagaat attcagcgtt
    15481 tccttaatga tttccccggt gccgaaacta ttcgtctgga gcaaaactac cgctctacca
    15541 gcaatattct gagcgccgct aacgccctga ttgaaaacaa taacgggcgt ctgggtaaaa
    15601 aactgtggac cgatggcgcg gacggtgagc ctatttccct ctattgcgct tttaacgaac
    15661 tcgatgaagc gcgttttgtg gttaaccgca tcaaaacctg gcaggacaac ggcggggcgc
    15721 ttgccgagtg cgccattctc taccgcagta acgcccagtc gcgtgtgctg gaagaagcac
    15781 tgttgcaggc gagtatgccg taccgtattt acggcgggat gcgcttcttc gaacgccagg
    15841 aaatcaaaga tgcgctctcg tatctgcgcc tgattgccaa ccgtaacgac gacgcggcct
    15901 ttgagcgtgt ggtgaatacg ccaacgcggg gtattggtga ccggacgctg gacgtggtac
    15961 gtcagacatc gcgcgatcgc cagttaacac tctggcaggc atgtcgtgaa ctgttgcagg
    16021 aaaaagccct cgccggacgt gctgccagcg ccttacagcg gtttatggaa ctgatcgacg
    16081 ccttagcgca ggaaactgcc gatatgccgc tgcatgtaca gactgaccgg gtaattaaag
    16141 actccggcct gcgtaccatg tacgagcagg agaagggcga aaaaggtcag acgcgtatcg
    16201 aaaacttaga ggaactggtg acggcaacgc gccagttcag ctacaacgaa gaagacgaag
    16261 atttaatgcc gctgcaggcg ttcctatccc atgcggcgct ggaagcaggc gaagggcagg
    16321 cggatacctg gcaggatgcg gtacaactga tgaccatgca ctcggcgaaa ggtctggagt
    16381 tcccgcaggt gtttatcgtc ggtatggaag agggcatgtt cccaagccag atgtcgctgg
    16441 atgaaggcgg gcgtctggaa gaagaacgcc gtctggccta cgttggcgta acccgcgcga
    16501 tgcagaaact gacgctgacc tacgcggaaa ctcgccgtct gtatggcaaa gaggtttacc
    16561 atcgcccgtc gcgctttatt ggcgagttgc cggaagagtg tgtggaagag gtgcgcctgc
    16621 gcgccacggt aagccgcccg gtcagccatc agcggatggg tacgccgatg gtcgagaacg
    16681 acagcggcta caagctcggc cagcgtgtac gccacgctaa gtttggtgaa ggcaccatcg
    16741 tcaatatgga aggcagcggt gaacatagcc gtttgcaggt ggcattccag ggccagggaa
    16801 tcaaatggct ggtggcagct tacgcccggc tggagtcggt gtaacgttgc cggatgcggt
    16861 gctgcgcacc ttatttggcc taaaaaatca ttcagattca ataaattgca acgtcatgta
    16921 ggccggataa ggcgtttacg ccgcatccgg caccagcagc atatttagta tctgacacaa
    16981 agctatcatt ctaacctttc cgctcgacgg aaatcatggt gacaaaagga tagcgttgcc
    17041 acgggattgc cccgcctttc atatacatat gtgaaatcgt gccatcaaga taaagcagct
    17101 gctcaacgtt cagtttcgct ttggcataac aggcaaaatc ataaaaattt gtcgcctgct
    17161 ggctcaacaa aaacacggca ttcccatgtt tattaatccc aacaccgtta cgaattttac
    17221 gtgaggcgac gttgggatga atacgcggat taattacacc gttttccatc aacattggtc
    17281 ctgactgcac cgcaaactga atatctttac tggctttaaa agcatccaga cgaacgatgc
    17341 cgactttatc tcccgcgaca taaaacacgc cgccaggacg gataaagaaa ttcccttcac
    17401 ctgaagcgag atttaacgcc accttc
//
LOCUS       DBKEKI010000075        17423 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.075, whole genome shotgun sequence.
ACCESSION   DBKEKI010000075 DBKEKI010000000
VERSION     DBKEKI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.075"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..238
                     /locus_tag="ACUQPX_002555"
     CDS             <1..238
                     /locus_tag="ACUQPX_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HHV9057236.1"
                     /translation="AIYLHWYQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWC
                     RALSFSPTKAKNYQRYNERIKGKRQEWGILCNND"
     gene            211..840
                     /locus_tag="ACUQPX_002556"
     CDS             211..840
                     /locus_tag="ACUQPX_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071453 - cellular response to oxygen
                     levels [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HHV9057237.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEDTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(841..2001)
                     /gene="ybdL"
                     /locus_tag="ACUQPX_002557"
     CDS             complement(841..2001)
                     /gene="ybdL"
                     /locus_tag="ACUQPX_002557"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HHV9057238.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            2110..3198
                     /gene="hcxA"
                     /locus_tag="ACUQPX_002558"
     CDS             2110..3198
                     /gene="hcxA"
                     /locus_tag="ACUQPX_002558"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HHV9057239.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(3208..3405)
                     /locus_tag="ACUQPX_002559"
     CDS             complement(3208..3405)
                     /locus_tag="ACUQPX_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HHV9057240.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3587..5681)
                     /gene="cstA"
                     /locus_tag="ACUQPX_002560"
                     /pseudo
     CDS             complement(3587..5681)
                     /gene="cstA"
                     /locus_tag="ACUQPX_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
     gene            complement(5862..6275)
                     /gene="entH"
                     /locus_tag="ACUQPX_002561"
     CDS             complement(5862..6275)
                     /gene="entH"
                     /locus_tag="ACUQPX_002561"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HHV9057241.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(6278..7019)
                     /gene="entA"
                     /locus_tag="ACUQPX_002562"
                     /pseudo
     CDS             complement(6278..7019)
                     /gene="entA"
                     /locus_tag="ACUQPX_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
     gene            complement(7019..7876)
                     /gene="entB"
                     /locus_tag="ACUQPX_002563"
     CDS             complement(7019..7876)
                     /gene="entB"
                     /locus_tag="ACUQPX_002563"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HHV9057242.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7890..9500)
                     /gene="entE"
                     /locus_tag="ACUQPX_002564"
     CDS             complement(7890..9500)
                     /gene="entE"
                     /locus_tag="ACUQPX_002564"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HHV9057243.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGHLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9510..10685)
                     /gene="entC"
                     /locus_tag="ACUQPX_002565"
                     /pseudo
     CDS             complement(9510..10685)
                     /gene="entC"
                     /locus_tag="ACUQPX_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10976..11932
                     /gene="fepB"
                     /locus_tag="ACUQPX_002566"
     CDS             10976..11932
                     /gene="fepB"
                     /locus_tag="ACUQPX_002566"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HHV9057244.1"
                     /translation="MRLAPLHRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGAATPNNRVADDQGFLRQWSKVAKERKLQQLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11936..13186)
                     /gene="entS"
                     /locus_tag="ACUQPX_002567"
     CDS             complement(11936..13186)
                     /gene="entS"
                     /locus_tag="ACUQPX_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HHV9057245.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            13297..14301
                     /gene="fepD"
                     /locus_tag="ACUQPX_002568"
     CDS             13297..14301
                     /gene="fepD"
                     /locus_tag="ACUQPX_002568"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HHV9057246.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            14298..15290
                     /gene="fepG"
                     /locus_tag="ACUQPX_002569"
     CDS             14298..15290
                     /gene="fepG"
                     /locus_tag="ACUQPX_002569"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HHV9057247.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPHSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            15287..16102
                     /gene="fepC"
                     /locus_tag="ACUQPX_002570"
     CDS             15287..16102
                     /gene="fepC"
                     /locus_tag="ACUQPX_002570"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HHV9057248.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(16099..17232)
                     /gene="wzz(fepE)"
                     /locus_tag="ACUQPX_002571"
     CDS             complement(16099..17232)
                     /gene="wzz(fepE)"
                     /locus_tag="ACUQPX_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HHV9057249.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4097 a         4948 c         4526 g         3852 t
ORIGIN      
        1 cgccatttat ttgcactggt atcagaaaaa aggcatcgag gttccacaaa cccagcaagg
       61 ggacattggc gcgaaagata tcccctcctg gcggcggata tgcaaagttt tactcaataa
      121 cgattactgg tgtcgggcat tatcatttag tcctacgaaa gcgaagaact atcagcgtta
      181 taacgaacgg ataaaaggaa aacgtcagga atgggggata ctatgcaaca acgattaacg
      241 caggatttaa cgcaatttct cgccagcttg ccggaagatg atcgcatcaa agcgattaat
      301 gaaatccgca tggcgatcca tcaggttagc ccctttcgtg aagagccagt ggattgcgtt
      361 ctgtgggtta aaaacagcca acttatgcct aatgattaca acccaaacaa cgtggcacca
      421 cccgaaaaaa aactgctgca aaaatccatt gaaatagatg gttttaccca acccattgtg
      481 gtcacacata cggataaaaa tgccatggaa atcgtggatg gttttcaccg ccatgaaatt
      541 ggtaaaggct catcatcatt aaaattacgc ttaaaaggtt atttgccggt gacctgtctg
      601 gaagatacgc gtaatcaacg catcgccgcg actattcgtc ataatcgcgc tcgtggacgc
      661 catcaaatca ccgccatgtc ggaaatcgtc cgtgaactta gccagttggg atgggacgat
      721 aataaaattg gcaaagagct gggaatggac agcgacgaag tgctgcgcct gaagcaaatt
      781 aacggcctgc aagagttgtt tgccgaccgt caatattccc gcgcctggac ggttaaatag
      841 ctaaagctgg cgcaggcgtt cagctgctgc cagcaacgtc gattccttct tggcaaaaca
      901 gagacgaatc agtttatgtg ggaagggatc ggcgcaaaac accgacagcg gaatcgccgc
      961 tacgccgtgc tcctgcgtca gccactggca aaactcaaca tcatccaggg tagaaaccgc
     1021 gctgtaatcc accagcaaaa agtatgtacc ttcacacggt aaaatctcca gccggctttc
     1081 atttaaagca ttcaccagaa tatcgcgctt ctggcgataa aagtccggta acgcaagata
     1141 atgctcaggt tctgcacgta gcatatcagc aagcgccagc tgtgccgggg tattcaccga
     1201 aaaggtcaga tactgatgta ccttgcgaat ttcggcgctg atgggcgctg gcgcaacaca
     1261 ataacccact ttccagccgg tcatatgata ggtcttgcca aatgaagaaa ccgccactgc
     1321 ccgctcacgc agctgcggat gcgccagcac actggcatgg ccctgttgtg aaaagttgat
     1381 gtgctcgtag acttcatcgc taatgacaaa aatctcgtgc ccggcgatcg cctgccacaa
     1441 agcggcgaaa tcagcctgct gccagacagt tgcactgggg ttatgcggag tgttgaggat
     1501 caccagtctg gtgcgctcgc ttaataatgc ggcaaattcc tgccagtcaa cgcgaaaatg
     1561 cggtggttgc agtgccatac gcttcactat tcccccagaa agcgcgatgg cgggggcgta
     1621 actgtcatag ctgggatcaa aacaaatcac ttcatcgcca ttgcgcacca gtgcggtaat
     1681 cgccgcgtat aacgcttccg tcgcccctgc cgttacggtg atatcgctat cggcatctgg
     1741 ttgatagcca tacaaacgtt ccgttttctg agcaatcgcc tcgcgcaagg cctgcacgcc
     1801 ggtcatgggc gcgtattggt ttgccccctg ggcaacgtgg tacgccagcc gctcctgtaa
     1861 atagcgcgga ccatcaaaat caggaaagcc ttgcgacagg ttaatcgcct ggtgttgctg
     1921 cgccagcgcg ctcatctggg tgaaaatagt ggtgccaagt tgtggaagtt tgctttgtgg
     1981 aatcagaggg ttatttgtca tttttattgt acctgacgaa gttgcgtgtt gaaggcacta
     2041 taacacgatg ctagtatttg gcaatcaaga cgtttagatg tctaaataaa acaataagga
     2101 caacacaaca tgcctcacaa tcctatccgc gtggtcgtcg gcccggctaa ctacttttca
     2161 catccaggaa gtttcaatca cctgcacaat tttttcactg atgaacaact ttctcgcgcg
     2221 gtgtggatct acggcaaacg cgccattgct gcggcgcaaa ccaaacttcc gccagcgttt
     2281 ggacttccag gggcaaagca tattttgttt cgtggtcatt gcagcgaaag cgatgtacag
     2341 caactggcgg ctgagtccgg tgacgatcgc agcgtggtga ttggcgtcgg cggcggcgca
     2401 ctgctcgaca ccgcgaaagc cctcgcccgc cgtctcggtc tgccatttgt tgccgctccg
     2461 acgatcgccg ccacctgcgc agcctggaca ccgctctccg tctggtataa cgatgccgga
     2521 caggcgctgc attatgagat tttcgacgac gccaatttta tggtgctggt ggaaccggag
     2581 attatcctca atgcaccgca acaatatctg ctggcgggga tcggtgacac gctggcgaaa
     2641 tggtatgaag cggtggtgct ggctccgcaa ccagaaacgt tgccgctaac cgtgcggctg
     2701 gggatcaata atgcgcaggc cattcgcgac gtcttgttaa acagtagcga acaggcactt
     2761 gccgatcagc aaaatcagca gttaacgcaa tcattttgcg atgtggtgga tgctattatt
     2821 gctggtggtg ggatggtcgg tggtctgggc gatcgtttta cgcgtgtggc ggcggctcat
     2881 gccgtgcata acggtctgac tgtcctgccg caaaccgaga agtttcttca cggcaccaaa
     2941 gtcgcttacg gaattctggt gcaaagcgcc ttgctgggtc aggatgatgt gctggcgcaa
     3001 ttaaccgggg cgtatcagcg ttttcatctg ccgactacgc tggcggagct ggaggtggat
     3061 atcaataatc aggcggagat cgacaaagtg attgcccaca ccttgcgccc ggtggagtcc
     3121 attcattacc tgccagtcac gctgacacca gatacgttgc gtgcagcgtt cgaaaaagtg
     3181 gaatcgttta aagcctgacg tgcaaattta gcagcagcgc gcgccgcctt ttccaccgta
     3241 gcgcgcgtcc tgccgctcac ggaaaaactc ctcgtaggtc atcggcgttt gatcgggatg
     3301 attaacccgc atatgttcga catagttgtc gtaatcaggc ataccaatca tcagcttcgc
     3361 cgcctgacct aaatattttc cggcttttgc cagtgaatca aacatcttgg cctccgctaa
     3421 ccatgttgct gaaagagtgc cggatgcggc gtgaacgctt tatccggcct acaaatgcta
     3481 tcaacgcact cggtcagtcc cctcgcccca tcggggagag ggttagggtg agggggaacc
     3541 ctcggcacta tgccaacatt cgccaacatc ccccctcact ctgactttag tgcgcgcctt
     3601 tcgcctgcgc tacgatctcc tcgacatttt ccggcatcgg ctcatacggc gtttctttcg
     3661 ccgttggctt cggatctttc aatgccgcaa gtgccgtctt aatcgagaac agtgccagaa
     3721 ccaccacgac caccataaag aagatggtta acccggcatc cagacggttg ttgaacacca
     3781 gttgcgccag ctgtgactca gtatactgcg acggaatatt gccgctgtcg atcattgcct
     3841 ggaacttgtt agcaatggcc aggaagccga ctttcgcatc cgggctaaac gctttctgcc
     3901 agtcagcggt cagggtacaa atcagcagcc aggccgttgg taccagcgcc acccaggcgt
     3961 aacgttgacg tttcatcttg aacaacacca cggcacagag catcagcgcc atccctgcca
     4021 gcatctggtt ggcaatacca aacagcggcc acagagtgtt aataccgccc agcggatcga
     4081 ccacgccctg atggaggaag tagccccacg ccagtacgca cagcgccgtt gccagcaggt
     4141 tagcaggcag tgaatcggtc cgcttcaggc caggagacac cacgcccagc agatcctgca
     4201 acataaagcg cgcagcacgc gtacccgcat ctaccgccgt cagaataaac agagcttcaa
     4261 acagaatggc gaagtgatac cagaacgcca catccatcat accacccagc gcaccgtgca
     4321 ggatgtaagc catccccacc gccaacgtcg gcgcaccgcc cgcacgggaa atgatcgact
     4381 gttcacccac ttcgctggca atctggttga gcgtatctgg tgtaatgcta aagccccagc
     4441 tactcaccat ctgcgcggca gaagcgacta catccgccgt ccctgccgga gccagcaccg
     4501 ccattgggct gttcatggca aaatacacgc ccggatcgat gatacaggca gaaaccagcg
     4561 ccataatcgc cacgaaggat tccatcaaca tcccaccgta gccgataaag cacgcctgcc
     4621 cttcgttcgc cagcatcttc ggcgtggtgc cggaagagat cagcgcatgg aagccagaca
     4681 ccgcaccaca ggcgatggtg ataaacagga acgggaacag gttaccggtc cataccgggc
     4741 cagtgccatc gacaaatttg gtcagcgcag gcatggtcag cgtcgggcgc ataatcaaaa
     4801 tgcctaccgc cagaccgacg atagtcccga ttttcaggaa ggtcgagaga tagtcacgcg
     4861 gggccagcag caaccacacc ggcagcaccg ccgccacaaa accgtagccc accagcatcc
     4921 aggtcagctg cacgccggta aagtcaaagt acggtgccca gtgccaccca gccgccagag
     4981 ataatggcga aaatcaggaa caccaggcca atcactgaca cttcgccaat gcggcccgga
     5041 cgcagatagc gcaggtagat tcccataaaa atcgccagcg gaatggtgaa cgcaacggta
     5101 tacgttcccc acgggctatg agtcagggct ttcaccacga tcatcgccag tactgcaagg
     5161 ataatgacca tgatcataaa gcaggctacc agcgcaatca ccccggcagt tggccccatc
     5221 tcttctttga ccagctcacc cagcgagcga ccgtcacggc gcgtagaaac aaacagcacc
     5281 atgaaatcct gcaccgcacc ggcgagaacc accccggcga gcagccagat catccccggc
     5341 aggtagccca tttgcgctgc cagcaccggc cccaccagcg gccctgctcc ggcaatggcc
     5401 gcaaaatggt gaccgaacag cactttcttg tccgtcggca catagtccag cccgtcgtta
     5461 tggcgcaccg ctggcgtcat acgcgtcggg tcaaccgcca gcacattttt ggcgatatac
     5521 agcccataaa aacggtaagc gatcagatag atacagaccg acgccaccac aatccacagc
     5581 gcgttgatct gttccccacg atttaaagca atgtatccca gagcaaatgc tcccattaca
     5641 gagagcactg tccagacgag gtatttccct gatttgttca tagttgttat ccgtgtgcgt
     5701 gtccatagag atgttacatt ttgtttctat aacatctttg tacgatttaa caacacggta
     5761 accatgtaaa tcggagttga aaccaatatt taacttaaca atgttaactc gatcactccg
     5821 ttaaccgagt tttcctgcaa tctcatttaa ttctgtcggg ctcatcccaa aactgccgta
     5881 cccagccgac aagtgcagca acgccgcccc tgttcatcga aaacgacgat ttcccagctc
     5941 tgattttgcc gcccaagatg cagcggctgg cagacgccgc gtaccttgcc ttcagacacc
     6001 gggcgatgat gggtggcgtt aagttccgtg cccaccacac actgcccgtc acgggtcatc
     6061 ataaatccgg ccatcgatcc cagcgtttca gccagcgccg ccgacgcgcc gccatgcagc
     6121 aggccaaacg gctgatgagt acgggtatca accggcattt cggcttccag cacatcatct
     6181 cccagacggg tatacacaat tcccagatgc gccaccattg tgttatcgct ggtggcgttc
     6241 agttcgtcga gcgttaaatg gcgtttccag atcatgctta tgcccccagc gttgagccgc
     6301 catcgaccac aatatcctgt agggtgatat ggctggcgag gtcagaggcg aggaacaaaa
     6361 tcgtgttggc gatctcttgt ggacgggcga ttttccccag cggaatgccg agtttaaact
     6421 gctcgccaaa gccgcgaata cgctgttctt cggcgtcatc gctcacccac agcgtgcgtt
     6481 gcatatcggt gtcggtggag ccaggcgaaa ccacattaca gcgcacgccg ctacccgcca
     6541 gttccagccc gacgctcaac gccaggcttt tcagcgccgc tttcgatgcg ccataagcac
     6601 tcataccaat acgcggcgtg tgtgcggcgt cggacgccac agtgacaatc gccccgctga
     6661 cggcgaaact ggttcatggt ttgctggaac aggttaaacg caccgccgac gttaaccgca
     6721 aaagtctgct gccagtcctc tttactgagc tgatcggtcg cgcccatgcg caaaattccc
     6781 gccgcattga tcagcacgtc cagtcgctcc gtttcagcta acagtcgctg acatacctgc
     6841 gccacctgcc cggcgtcggc aacatccatt acttcggtcg caaagggata ttgctcctga
     6901 gtgaacgctt gatcaaaacc tgtaactttc gctcccgcct caacaaacgc cagcgccgtg
     6961 gcgtagccga tacctttacc tgcgccggtt acccagacat ttttaccgct gaaatccatt
     7021 atttcacctc gcgggagagt agcttccacc aggcgtcgat ggtcgggttt ttcgccagca
     7081 tgacaaagtc gatatcacca tgcactttgc gccagcgcgc cgccagcgcc atcatgcgca
     7141 ccgaatccag accgtagtcg atcaggttgt cgtcatcgaa cggttcatcg gactcgtcca
     7201 gcaacggcag aatcacctca cgcagcgccg ctttgctggc ggggataggt gctggcagta
     7261 attcttcggt catcaccacc cggccagaac gtccggccac atatttcagc gacatcaaat
     7321 gctcgtcacg gctgaaatca gccagcgcat ccgccaccat aaacggttta atatcgcgca
     7381 taaatgcgtc ggttgcggtg gtcatacagc caatgtgggc atatacccct gtaataatca
     7441 gctggttacg tccactctct ttgagcattt gctccagcgg agaacgatga aacgcgctgt
     7501 agcgccactt caccagcacc gtgtcgtcgg catctggcgt caggcgatcc accacctttt
     7561 gctgttccgg cgagcgggtc aggcccggcc cccacatatc attcaacagc gcccgatctt
     7621 catcgctctg ctctttcggc tgggcggtgt aataaaccgg gatattgtgc tgtttgcagt
     7681 agtcgcgcag cgcagcaata ttcgcgatca cctgctccat catcgggcag ttctcgcccc
     7741 agaagctgac aaaatagtcc tgcatatcat ggattaacaa cgcggcacgt tgcggttcaa
     7801 aggcccagtc aactttattc tgcggaatat cgtgagactc cggcagtgcg taagcctgta
     7861 attttggaat agccatcgtg ttctctcctt caggctgatg cgcgtgacgc cagccactga
     7921 cgtaattgtt ttttatcgac tttcccgacc gccgtcagcg gaagtgaatc cacacactcc
     7981 acgcgatccg gtaatttaaa ttcggcaata ccctgttcac gcaggaaacg acgcacctgc
     8041 accgcgcgca gcggctcttt taccaccaga taagcacagc ttttttcgcc catcagctca
     8101 tcttccatgc tcaccagtgc ggcatagatc actgccggat ggcgcagcag caggttttcg
     8161 atatcttcgg cagcgatctt ctcgccgcca cggttgatct gatctttctc gcgcccctgc
     8221 acggtgatgt aaccctcagg atcaatagag atcagatcgc cggaacagta aaaaccgttg
     8281 gcatcaaagg cactggcatt gtgctgcgga cttttgtaat agccacggaa ggtgtacggc
     8341 ccgcgcgtca tcaggtgtcc aacttcccct tgcggcagtg gatttccttc ggcatcggca
     8401 acccacactt catcgtccgg acacattggg taaccctggg tatggataat tttctccgcg
     8461 ctatcatcaa gacgggtgta gttcaccagc ccttccgcca tgccaaacac ctgctgcaac
     8521 tggcagccaa tctcagcggg aatacgcgct gcaagcgtgg cagaaagacg tgcgccgccg
     8581 acctgtaaca gtttcagcga ggcaagctgc gcccggcttt cgccttcagc cagcgcctgc
     8641 aaccacaggc tgactgccgg cggcaccagc gcggtaacgt taacctgatg ttttgcaatc
     8701 aacgggaagc aaagcgtggc gctgggatcg gccgccagaa caaccgttcc tccggcaaga
     8761 aagacgccca gcgatcctgg cgaactcata gcgtagttat gagccgccgg gatcgcgcac
     8821 aggtagcgcg tctgttgtgt gaactgacaa atctcgacgc tacgacgcac gctgtagtaa
     8881 tagtcgttat gagtgcgcgg gatcagtttc ggtgtgccgg tggtgccgcc ggaaagctgg
     8941 aaataggcca cttcatcagc aggtgatggc gtggcagtaa aaccctctgc cggatggtta
     9001 atcgcatcct gcaagttatg ctcaccgctg tcgttgtgca gttgcaccac gcgaatggaa
     9061 gaatgctctg caacaaatgt gttgaggaaa tcatccccgc taaatagcgc atgctggcga
     9121 tcggcaatca gtaacgccgg ttcaatctgg ctggcgtagg cgttcagttc actacgctga
     9181 tggctgaaca gcgccagcac cggcgcgacg cccagtttca gcagcgcgaa aaaggtaatg
     9241 taaagttcag cgacgttacc cagttgtacc agcgcggttt caccaggttt aatgccctga
     9301 cggcgcaaac tacaggcgag gttatccgcc gcctgattca gctcccgata actcaactgc
     9361 cgctcgccgt cgataaccgc gatgctgtca ctcgcagcgt ggcgagtcag aatgtcggtc
     9421 agtggcaaat cctgccagta gcctttttcc cgatagcgac gggcaaactc ttccggccag
     9481 cgggtgaatg gaatgctcat cctcgctcct taatgcaatc caaaaacgtt caacatggta
     9541 gaaagtttga cgcctgtttc gcgccactca cccaacggtg acgacgcagg cacaatcccc
     9601 gctccggcaa acagacgcac ctgattttcc cgctgcttcg cgcagcggat ggtcaccacc
     9661 cattcgccgt taccttcgct gtcacaccaa cccacaatgc cgccaaacag ttcgcggtcg
     9721 aacggctcca gttcagcaat aacctgggtc gcggcctgat gcgggaaacc gctcagcgcg
     9781 ggggtcggat gcagcagaca ggccagagtc agtgcgtttt cttgcgaatt cgctttacct
     9841 tcaaagggag ttgcgagatg ccacagcgtc ggcgtggtaa tcaattgtgg agaggaggga
     9901 acgtgtaact cactactgcg ttcgcgcagt acctctttca tcgcctgagt caccagttca
     9961 tgttcatggc gatctttttc tgacgccagc agacgattac ccgcttcgcg atcgagcact
    10021 tcatccggct gacgacgcgc ggaaccggct aacggaatgg agctaaaacg ctcgccgtct
    10081 ttacgtagca gcagttccgg gctggccccc agcaggacgc caccatcagc cagcgggaca
    10141 tggaagttgt aactaaccgg gttttgcgca atcaaccgtt ccagcaatac gccactatca
    10201 atggcggcgt cagtggtgat atcaatcaat cgtgacaaca ccactttgtc gacctgcggc
    10261 gtggcggtaa gtgcggcggc gcgggcaccc atctgttcaa acgtggtttg ttcaggaatt
    10321 gcctggcgtt ccaccacgtt cagcgactgg ctgcgggtaa aacagcgggc tgaggtctgt
    10381 ttttcctgac gggagaacga ctgctaggat tcgggaatat acagcgacga aggctgacgt
    10441 ggatcgaagg gaatcgcccc gaccatcacc ggatttttga tgccctgcgc tttggcatcg
    10501 gcaaacagcg cggcgagttt ttgctggaag ggactgtcgg gcgaatcccc gttcacagcc
    10561 ggttcatcga aacgggcgaa acatcctgac gtcgtaaaac tgcggtacgg cgatataaag
    10621 aaaaagcgat tgggcgcaag tgttgccatg gtctgctgta cttcctcagc cagtgacgta
    10681 tccatatcat cctccacaaa tgataaaggc tttaataatg attatcattt atattttcgt
    10741 ccgctaacct aaaagcaaac gcgcactatg tcaactcttg aggtaacgca atctgcaact
    10801 cggggttgca tctggttgtg gttacaatga aaatgagaag cattattgat gaattcgcat
    10861 aagcgcaatg tgatgtcctg cgccgttttg ccccctctcc ccaaagggac ggattgtgcg
    10921 ctttgtcgaa tttgtcatta cgcccttaac cttattaata acaggaagct gacttgtgag
    10981 actcgccccg ctccaccgca acgcccttct attaacagga cttttgcttt caggaatagc
    11041 cgtagttcag gccgccgact ggccgcgtca gattactgac agccggggca ctcataccct
    11101 ggaaagccag ccgcagcgta ttgtttccac cagcgtcacc ctgaccggat cactgctggc
    11161 gattgatgct ccggtgatcg ccagcggcgc ggccacgccg aataaccgcg tcgcagatga
    11221 ccagggcttt ttacgccagt ggagcaaggt ggcgaaagaa cgcaaactgc aacagctcta
    11281 tatcggcgaa ccgagcgccg aagccgttgc cgcgcaaatg ccggatctga ttttaattag
    11341 cgcaaccggc ggggattcgg cgctggcgct gtatgatcag ctttccacca tcgccccgac
    11401 attaatcatc aattacgacg acaaaagctg gcagtcgctg ttaacgcagc ttggcgaaat
    11461 taccgggcat gagaaacaag cggcagagcg gattgcacag tttgataagc aactggcagc
    11521 ggcgaaagag caaatcaaat taccgccgca gccggtcact gccattgtct ataccgccgc
    11581 tgcacacagt gccaatctct ggacgccgga atcagcacaa gggcagatgc tggaacaact
    11641 cggctttacg ctggcgaagt tgcccgcagg gttaaacgcc agccaaagcc agggcaaacg
    11701 ccatgacatc attcagcttg gtggggaaaa tctggctgcg gggttaaacg gtgaatcgct
    11761 attcctattc gctggtgatc agaaagatgc cgatgcgatt tatgccaatc cactgctcgc
    11821 gcacctgcct gcggtacaaa acaagcaggt ttatgcgctg ggaaccgaga cgttccgcct
    11881 ggattactac agcgccatgc aagtgctgga acggcttaag gcgctgtttt aagcattaac
    11941 tgtcggacgc tgtcacctgc ggcggcgtct ggcgaaaacg tcgcaactcc accagcacca
    12001 gcaataacaa cacgccgata atcaacaaac caaaaccgct cgcgcttgcg gaagcaaccg
    12061 gtgtcatcat cgcgcccaaa ccacccagca gcgccgcgcc tatggcatcg cccgtcacgt
    12121 tctgcgccgt ccacaaaccg ttaatccgcc ctaacatcgc ttccggggtt tgcgtttgca
    12181 gcattgtata ttgcagcaac gaactaaccg cgctcagcca gccgaacagc gccagacaaa
    12241 ccacgcctaa aatccacatc ggcatcaggc caaacagacc aatggcgagg aacgatccca
    12301 gcgtggagag cagcatcaat aaccctggtc gcgcactatg tgccagcttc ccgctggtta
    12361 acgcaccaat agccgcgccg agcgggatcg ccgcgtagag aaaaccaatc tgtgccgctg
    12421 acatctgcca gttgtcagcc agcgccggat acagtaccag caccgcgctc gccatcgtca
    12481 ataaaccacc cagcagcgca atcccgccca ccagcgggct ggcaagcaga aaacgaaatc
    12541 ctgccagtaa tgatttcaac ggatgctcac gcggctgcgg tggcggtggc aacgccggaa
    12601 ggcttaataa cggtaacaag gtaataaacg tgcccgccgc cgccagcccg tagttccagg
    12661 ctacgccgcc ggtcgccagc aataaaccgc caatcatggg cgaaatcacc gaccccaggc
    12721 gcacggtcaa catggtgatc gccccggcct gcattaagtt ttcacgccct accagtgcag
    12781 atgttgccgc cagtagcgcc gtaacaccaa gtgatgcgaa aaaaccatcc cataaaccaa
    12841 gtaaatagat tgccagcaat gacggctccg gcagcagtgc attaaggcac agtccaatga
    12901 agccaatgcc acaggtgccg cgcgccagca aaatcacttt tttgcgttca tagcgatccg
    12961 ccagcacacc gccaaccatc aggccaacaa acatcgcgcc gccggtcagc gtcaccgaaa
    13021 gccccacctg ccaggtagaa tgggtcatca tctggatctg caccggcacc gcgacgccga
    13081 gcaaacccag agacacaatt gagataaaac gagcgaggaa tactgcgcga aacgccgggt
    13141 gcgttttcaa caggctgagg ttaagcagcc aggattgttt attcattaca atgccttgcc
    13201 atcaaatatt ttaaatacct attcttaggc tgcacatgct aacatatcca aataagatcg
    13261 ataacgataa ttaatttcat tatcatggaa gttcgtatgt ctggttctgt tgccgtgaca
    13321 cgcgccattg ccgtgcccgg attgctgtta ttactgatta tcgcgacggc attaagcctg
    13381 ctcattgggg caaaatcact ccccgcttcc gtagtgctgg aggccttctc cggcacctgc
    13441 cagagcgccg actgcaccat cgtgctcgac gcgcggctgc cgcgtaccct tgccggttta
    13501 ctggcaggcg gcgcgcttgg ccttgccggg gcgttaatgc aaaccctcac ccgaaaccca
    13561 cttgccgacc ccggcttgct tggcgtgaac gccggagcca gctttgccat tgtgctgggt
    13621 gcggcgctgt ttggttactc atccgcacag gaacaactgg cgatggcctt cgccggggcg
    13681 ctggtggcct cattgattgt tgcctttacc ggcagtcagg gtggagggca gttaagtccg
    13741 gtgcgtttaa ccctggcggg cgtggcgctg gcggcggtgc tggaaggact gaccagcggc
    13801 atcgccctgc ttaatcctga cgtctacgat caactgcgtt tctggcaagc cggttcgctg
    13861 gatattcgca atctacatac cttaaaagtg gtgctgatcc cggttctgat cgccggagca
    13921 actgcgctat tactgagtcg cgcgctgaac agtttgagcc tcggcagtga caccgcgacg
    13981 gcgctgggca gtcgcgtggc gcgcacacag ttgattggtc tgctggcgat taccgtgctt
    14041 tgtggtagtg cgacggcggt agttggcccg attgccttta tcggcctgat gatgccgcac
    14101 atggcgcgct ggctggtagg tgccgatcat cgctggtcgc tgcccgtcac gctacttgct
    14161 acccctgccc tgctgctgtt tgccgatatc atcgggcggg tgattgtgcc cggcgaactg
    14221 cgcgtttctg tggtcagtgc gtttattggt gcaccggtgc tgatcttcct cgtgcgacgt
    14281 aaaacgcgag gtggtgcatg atttacgtct ctcgccgatt actcatcacc tgtttgctgc
    14341 tgatttccgc ctgtgtggtt gcaggtatct ggggattacg cagcggtgcc gtcacgctgg
    14401 aaatctcgca ggtattcgcc gcgttgatgg gcgatgcgcc gcacagtatg acgatggtgg
    14461 tcaccgaatg gcgtttacca cgcgtgctga tggcgctgtt gattggcgcg gcgctgggcg
    14521 tcagcggcgc gatttttcag tcgctgatgc gtaacccgct cggcagccct gacgtaatgg
    14581 gctttaacac cggggcgtgg agcggcgtgc tggtggcgat ggtgctgttt ggtcaggatc
    14641 tgacggctat cgcgctggca gcaatggtgg gcggcattat cacttcgctg ttggtctggc
    14701 tgctcgcctg gcgtaacggt atcgacacct ttcggttgat tattatcggt atcggcgttc
    14761 gcgccatgct ggtggccttt aatacctggc tgttgctgaa agcgtcttta gaaacggcgc
    14821 taacagcagg tttgtggaat gccggatcgc tcaacggcct gacgtgggca aaaacctcgc
    14881 cttccgcacc catcattata ttgatgctca ttgccgccac cttgctggtg cggcgaatgc
    14941 gcttgctgga gatgggcgat gataccgcct gcgcgctggg cgtcagcgtc gagcgttcgc
    15001 gtctgttaat gatgctggtt gcagtggtgc ttaccgctgc ggcaacggcg cttgccgggc
    15061 cgatttcctt tattgcttta gtcgcgccgc acattgcccg acgcattagc ggcaccgctc
    15121 gctgggggct gacccaggcg gcgctgtgcg gagcgctgtt actgctggcg gccgatctct
    15181 gcgcccaaca actgtttatg ccgtatcaac ttccggttgg cgtcgttacc gtcagcctcg
    15241 gcggtattta ccttatcgtc ttgttaattc aggagtctcg caaaaaatga ccgaatcagt
    15301 agcccgtttg cgcggcgaac agttaaccct gggatatggc aaatataccg ttgcggaaaa
    15361 tctgactgta gaaattcctg atggtcactt tacggcaatt atcggaccaa atggctgtgg
    15421 taaatccacg ttactgcgta ccttaagccg cctgatgacg cctgctcacg ggcatgtctg
    15481 gctggatggc gagcacattc aacattacgc cagtaaagaa gttgcacgcc ggattggtct
    15541 gttggcgcaa aatgctacca cgccgggcga tatcaccgtg caggagctgg tggcgcgtgg
    15601 acgttatccg catcaaccgc tgtttacccg ctggcgcaaa gaggatgaag aagcggtaac
    15661 gaaagcgatg caggccacgg gaataactca tctggcagat caaagcgtgg ataccctttc
    15721 tggcggacaa cgccagcgag cgtggatcgc gatggtgctg gcccaggaaa cggcaattat
    15781 gctgctcgac gaacccacga cctggctgga tatcagtcat cagattgatc tactggaact
    15841 gttaagcgaa ctgaaccgcg agaaaggcta taccctggcg gcggtgctgc acgatcttaa
    15901 tcaggcctgt cgttacgcca gccatttgat tgcattgcgg gaagggaaaa ttgttgctca
    15961 gggagcgccg aaggagattg tcactgctga actgattgag cgcatttatg gtctgcgatg
    16021 catgatcatt gacgatccgg tagccggaac gccgcttgtg gtgccgctcg gacgaacggc
    16081 accttcaacc gcaaatagtt aaactaagtg gtctgccatc atggcatcct gttttctgga
    16141 tgccatcgca tagcgcaata acacgctacc acaagccacc atcccgccga tcagcgcgga
    16201 aaggatcaca ataatcgcct tacccggacc gtcttttttc actggcaatg acgggcttaa
    16261 ctgatattta aacggcgtaa aattcacatc gttgacattt gcttttgtta attgctcgac
    16321 aagatactgc cgattacgta attcaccgtt cagttccgca acgtcagtaa ccgctttttc
    16381 aatttccagt ttgcgttcaa taccgtctgc gccgagagaa atagagaaat cggggtcatc
    16441 tttaacggcc tgaccattac tgtatacggg ctttttaatt cctgccgcgt tggcaatgtc
    16501 gagtgaataa ttgaggcgct gaatgtttgc atcaagttga tttttcgttt taatgcgatc
    16561 ctgagccagt ttttcttttt caaactgggt tttgatctcc agtttattac ggacgttttc
    16621 tatcgactct ttcaccacca acgcagagat ataatcgata tacccgctca aaacggtctg
    16681 cgcctcttta ctggttggcg cggtaaaact tagcgtccag gaggtataca gtgacggttc
    16741 atcttttttc ttactggcat tgtcatcaac cgctttcatt ttttcgctca atgcaacaat
    16801 agcgcgatgc aaatccagtt catcgatttt cgcctctttt aattggtcca tcacataagg
    16861 tgatgaacgc aggtactctt ccagcaagct aacagactga aacttcttga taaacaggtt
    16921 aaatgcaccg ccacggtcga tattaacatc gagatcaaga acgcggagtt tggtaaaggt
    16981 tttctccagg tcctgccact gaatggcttc ggcgggagta ataacggctg agctggtcca
    17041 tttttgcggc aggatgaaag agatcagtaa acctgcgcag gcaaatgcaa aaacgaccgc
    17101 catgaccgtt tttttggccc gccataaaac ttcgattaga ttaagtaaat caatttcatt
    17161 attactgggc gacgccagag gatactcaga aaaatgagcg tcacttccct gtttaatatt
    17221 caatgatgac atggggtata acctgaaaat gagtccaata ggggaaaatc tccacggcgc
    17281 tcactttacc agttggtgag aattatccga agctgaagtt tgtaaattcc ttccactgaa
    17341 tgcaacattc actctattta agagaaatat tcatcaaacc tataaatcaa acaaataaaa
    17401 aacccataat tattttctgt aat
//
LOCUS       DBKEKI010000076        17379 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.076, whole genome shotgun sequence.
ACCESSION   DBKEKI010000076 DBKEKI010000000
VERSION     DBKEKI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.076"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(35..766)
                     /gene="rlmB"
                     /locus_tag="ACUQPX_002572"
     CDS             complement(35..766)
                     /gene="rlmB"
                     /locus_tag="ACUQPX_002572"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HHV9057250.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     TGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(946..3387)
                     /gene="rnr"
                     /locus_tag="ACUQPX_002573"
     CDS             complement(946..3387)
                     /gene="rnr"
                     /locus_tag="ACUQPX_002573"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HHV9057251.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKSGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3426..3851)
                     /gene="nsrR"
                     /locus_tag="ACUQPX_002574"
     CDS             complement(3426..3851)
                     /gene="nsrR"
                     /locus_tag="ACUQPX_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HHV9057252.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4056..5354)
                     /gene="purA"
                     /locus_tag="ACUQPX_002575"
     CDS             complement(4056..5354)
                     /gene="purA"
                     /locus_tag="ACUQPX_002575"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HHV9057253.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5458..5655)
                     /locus_tag="ACUQPX_002576"
     CDS             complement(5458..5655)
                     /locus_tag="ACUQPX_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HHV9057254.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5737..6741)
                     /gene="hflC"
                     /locus_tag="ACUQPX_002577"
     CDS             complement(5737..6741)
                     /gene="hflC"
                     /locus_tag="ACUQPX_002577"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HHV9057255.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWCISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6744..8003)
                     /gene="hflK"
                     /locus_tag="ACUQPX_002578"
     CDS             complement(6744..8003)
                     /gene="hflK"
                     /locus_tag="ACUQPX_002578"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HHV9057256.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8089..9369)
                     /gene="hflX"
                     /locus_tag="ACUQPX_002579"
     CDS             complement(8089..9369)
                     /gene="hflX"
                     /locus_tag="ACUQPX_002579"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HHV9057257.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSTAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9445..9753)
                     /gene="hfq"
                     /locus_tag="ACUQPX_002580"
     CDS             complement(9445..9753)
                     /gene="hfq"
                     /locus_tag="ACUQPX_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HHV9057258.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9839..10789)
                     /gene="miaA"
                     /locus_tag="ACUQPX_002581"
     CDS             complement(9839..10789)
                     /gene="miaA"
                     /locus_tag="ACUQPX_002581"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HHV9057259.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10782..12629)
                     /gene="mutL"
                     /locus_tag="ACUQPX_002582"
     CDS             complement(10782..12629)
                     /gene="mutL"
                     /locus_tag="ACUQPX_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HHV9057260.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIAPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12639..13976)
                     /gene="amiB"
                     /locus_tag="ACUQPX_002583"
     CDS             complement(12639..13976)
                     /gene="amiB"
                     /locus_tag="ACUQPX_002583"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HHV9057261.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQEQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13995..14456)
                     /gene="tsaE"
                     /locus_tag="ACUQPX_002584"
     CDS             complement(13995..14456)
                     /gene="tsaE"
                     /locus_tag="ACUQPX_002584"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HHV9057262.1"
                     /translation="MMNRVIPLPDEQVTLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14428..15975)
                     /gene="nnr"
                     /locus_tag="ACUQPX_002585"
     CDS             complement(14428..15975)
                     /gene="nnr"
                     /locus_tag="ACUQPX_002585"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HHV9057263.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15974..17113
                     /gene="queG"
                     /locus_tag="ACUQPX_002586"
     CDS             15974..17113
                     /gene="queG"
                     /locus_tag="ACUQPX_002586"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HHV9057264.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17096..17149)
                     /locus_tag="ACUQPX_002587"
     CDS             complement(17096..17149)
                     /locus_tag="ACUQPX_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057265.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3936 a         4959 c         4368 g         4116 t
ORIGIN      
        1 ccaaatctgg atggcttttc ataattcaga ggaactagct gcgctgacga accgcttcaa
       61 ataagcaaat tccggttgca accgaaacgt tcagggaaga aacacttcct gtcattggga
      121 tgctgatcaa ctcatcgcaa tgttcacggg tcaggcgacg cataccttca ccttccgcgc
      181 ccatcaccag cgccaggcgt ccggtcattt tgctctgata gagtgtatga tccgcctcgc
      241 ctgccgtacc gacgatccag atattctctt cctgcaacat acgcatggtg cgcgcaaggt
      301 tagtcacccg aatcagcgga acgctttctg ccgcgccgca ggctactttt ttcgccgtag
      361 cgttgagctg tgcggagcga tctttcggca caatcaccgc atgaacgcct gcggcgtccg
      421 cactacgcag gcacgcgccg aggttgtgcg gatcggttac gccgtcgagg atcagcagga
      481 acggttgatc gagcgaagcg atcagatccg gcagatcgtt ttcctgatac tgacgtcctg
      541 gcttcacgcg ggcgataatg ccctgatgca cggcaccgtc gcttttctcg tcgagatatt
      601 ggcggtttgc caactggata accacgccct gagactcaag ggcgtgaatc agcggtaaca
      661 gacgtttatc ttcacggcct tttaaaataa agacttcctg aaaacgttca ggggcgcgct
      721 ccagcagggc ctgcactgcg tggatgccgt aaatcatttc gctcattaat gtactcgttg
      781 tttacgtttt gcctgatgca ctacgtttat ccggcctaca tgatctctgc aatatattga
      841 atttgcgtga ttatgtaggc cggataaggc gttcacgccg catccggcat gaacaaagcg
      901 cattttgtca gcaatctaac cctcttcttt taaagagggt attgatcact ctgccacttt
      961 tttcttcgcc gcacgcttcg ctttggtcgc tgcggctatt ttctgcgttt tcgccgatgg
     1021 ctttttcgct tttctcgcgt ctttcttcgc cgctttcggc ttcgtttttt tctcaccgcg
     1081 gaaggcgctg tctggctcaa agtttacctt tttaccgacc tgacgacgct tgccgccttt
     1141 tttgcctgca tcgccttttt tcgctttctc gcgcgccgtt ttaccgacgt tgcgcggtgc
     1201 gcgttcactg gagatcagac taaagtcgat tttgcgctcg tccatattaa ccgcttcgac
     1261 gcgaacttcc acgcgatcgc ccaggcgata agtctggccg ctggattccc ccatcaggcg
     1321 ttgccctacc tggtcaaagc gatagtagtc attgtccagc gaagagacat ggaccagacc
     1381 atcaatgaac aagtcgtcca gacggacgaa gaagccaaag ccagtgacgc tggaaattac
     1441 gcctttaaag acgttaccta cctggtcgag catgaagtca cacttcagcc agtcagcgac
     1501 atcgcgcgtt gcttcgtcgg cacgacgttc cgccatcgaa cagtgctgac ccagttgcaa
     1561 catctcttcc atcgaataat ggtagccgcc ggtttcagtg gtgttgccct gatgcccctg
     1621 ctctttcgcc agcagatatt taatggcgcg gtgcagcgtc aggtcaggat aacgacgaat
     1681 cggcgaagta aagtgcgcat aggactgcaa tgccagaccg aagtgaccac ggttttctgg
     1741 atcgtaaatc gcctgtttca tcgagcgtag cagcatggtt tgcagcattt ctgcatcagg
     1801 acggtcggca accgactcca gcagttccgc gtagtcacgc ggttccggct tgttaccacc
     1861 cggcagctcc agccccagct ccgccagcac tgaacggaaa gaggtaatcg cttcggtgct
     1921 cggcttgtcg tgaatacgga acagtgccgg ttctttcgct ttctcaacga aacgcgccgc
     1981 cgagatattc gccagaatca tgcactcttc aattaatttg tgcgcgtcgt tacgctgggt
     2041 ctgttcgata cgttcaatac ggcgttcagc gttgaaaatg aacttcgctt cttcgctctc
     2101 aaatgagatc ccaccgcgtt cttcacgggc tttatccagc actttataga ggttatgcaa
     2161 ctcttcgaga tgcttaacca gcggggcgta ctgctcgcgc agatcctgat cgccctgcag
     2221 aatatgccag actttggtgt aggtcagacg cgcgtgagag ctcatcaccg cttcgtagaa
     2281 tttgtagccc gtcaggcggc ctttcgacga aaccgtcatc tcgcacacca tacacaggcg
     2341 gtctacctgc gggttgagcg aacacaggcc gttagagagc acttccggca gcatcgggat
     2401 aacctgcgaa gggaagtaca ccgacgtgcc acggttacgc gcttctctgt ccagcggcgt
     2461 tggcggacgc acatagtagc tgacgtcggc aatcgcgacc cataaacgcc agccgccgcc
     2521 gcgttttttc tcgcagtaaa ctgcatcgtc aaagtcacgg gcgtcttcgc catcaatggt
     2581 gaccagcggt aaatcgcgca aatcgacacg gcccgatttt gcttcttccg gcacttcttc
     2641 tttcagcccc gcaacctgtt gctcaacagc ctgcggccag atatacggaa tttcatgggt
     2701 acgcagagcg atatcaaccg ccatgccggt gcccatattg tcgcccagca cttcgacgat
     2761 tttacccacc gctttggtgc ggcgagtcgg acgctgagtc agttcgacta cgaccacaaa
     2821 gcccatccgc gcgcccatga tctgatcggg cgggattaag atatcgaagc tcagacggct
     2881 atcgtcagga accacaaagc cgacgcccgc ttcggtaaag tagcgaccaa caatctggct
     2941 ggtttttggc accagtacgc ggacaatacg cgcttcacga cgacctttac ggtcagcacc
     3001 cagcggctgc gccagcacct gatcgccatg aatgcaggtt ttcatctgct cgctggagag
     3061 atacaaatca tctttacgcc cttcaacccg cagaaagccg tagccatcac ggtggccaat
     3121 aacggtacct ttcaccaggt cgaggcgttc cggcagcgca tagcactgac gacgagtgaa
     3181 gaccagttga ccatcgcgct ccatcgcgcg caggcggcga cgcaggcctt caagctgctc
     3241 ttcgccttca atgtgcagtt ctaccgccag ctcatcacgg ctggccggtt tttcacgttt
     3301 ggttaaatgt tcgaggataa attcccgact agggatggga ttcgcgtatt tttcagcttc
     3361 gcgttcctgg aaaggatctt gtgacatctc ggttcctccg ttgtcatctc tgatgaagat
     3421 tttcgtcact ccaccagcaa taatttataa agcggttgat tctcttcaac caaatcggca
     3481 agcgtgtagt tatccagttc cgtaagaaaa ctttgcacgg ccttagaaag cgcctgtttc
     3541 aaccgacagg caggtgtaat gtggcaaaac tcactgctgc aattcaccag cgataagggc
     3601 tccagctcgc gcaccacatc accaatacgt atcgcactcg ccggtttacc caggcgaatg
     3661 ccgccatttt ttccacgaac agcagtcacg tagccggcac gactaagttg attgattatt
     3721 ttgaccatat gattacggga gacgccgtag acgtcagtca cttcagaaat actggtcatc
     3781 cgcccttctg gcaatgacgc catgtagatc agcgcacgta atccgtaatc agtgaaactc
     3841 gttaactgca catcaacctc aaaagggaaa tcgggaaaaa atacatttat attgatgata
     3901 aaccagccac aagctgtgtc gctaatttat ttcagttcgg ggaaggaaaa aagcgaggat
     3961 ttaacactgt gtcggatagc gtaatggctt aaccgacaca gcaatgtatc acactgttag
     4021 cccggcaggc aaaatatctg ccaggcgtac cagaattacg cgtcgaacgg gtcgcgcaga
     4081 atcatggttt cagtacgatc cggaccggta gagatgatat cgatcggcac accggtcagc
     4141 tcttcaatac gcttgatgta gttcagcgcc gcctgcggca ggccgctacg atctttcacg
     4201 ccgaaagtgg attcagacca gcccggcatg gtttcgtaaa tcggctctac acctttccag
     4261 tcgtcagctg ccagcggagt ggtagtcact tcgcgaccat ccggcatacg gtaagccacg
     4321 cagagtttaa cctctttcag gccatccaga acgtccagtt tagtcaggca gaagccagac
     4381 agggagttca gctgtaccgc acgacgaacg gcaacggtgt ccagccagcc ggtacgacga
     4441 cgacgacccg tagttgcacc gaattcgtta ccctgcttgc agaggaactc gccagtttca
     4501 tcaaacagtt cggccgggaa aggacctgca cctacacgag ttgagtaagc tttgaggata
     4561 cccagaacgt aatcaacata acgcgggccc aagccggaac cggtcgccac gccaccagca
     4621 gtggtgttgg aagaagttac gtacggataa gtaccgtggt cgatatccag cagcgtaccc
     4681 tgcgcacctt cgaacatgac gaaatcgcca cgctgacgcg cctggtcgag caggtcagaa
     4741 acgtcaacca ccatagaagt caggatgtcg gcaacagcca tcgtatcatc cagaactttc
     4801 tggtaatcaa ccgcttcagc tttgtagtag ttaaccaact ggaagttgtg atattccatc
     4861 acttctttca gtttttcagc gaaggtttct ttgtcgaaaa ggtcgccaac acgcagaccg
     4921 cgacgtgcca ctttatcttc ataagcaggc ccgataccac gaccggtggt gccgatcgct
     4981 ttcgcgccac gcgctttctc acgcgcgtta tccagcgcaa cgtgataatc aaggatcagc
     5041 ggacatgctt cagacagcag cagacgctca cgaacgggga tgccacggtc ttccagttct
     5101 ttcatctctt tcatcagcgc ggccggagac agcacaacac cgttaccgat gatgctggtt
     5161 acattctcgc ggagaatacc tgatggaata agatggagaa cggttttttc accgttgatt
     5221 acgagagtat ggcctgcgtt gtgaccgccc tggtagcgta caacatattt agcccgttca
     5281 gtcagaagat cgacgatctt acctttacct tcgtcacccc attgggtgcc cagtacgacg
     5341 acgttgttac ccatttttca aaatcaccgt ttgcttaaaa atggattcta ccatcgcttt
     5401 ttcagagtta cagcactttt tgcactcaaa aatgacctcg atcagtctgt tttttgttca
     5461 gccaatcgtt ttcctcaaca tgtagtagac cacaacgccc gcaaccacaa gtccaccgcc
     5521 aaaacgacgt aaaatattat cgggcaaatt ggtcatcgca gagatcatct tcttccatgc
     5581 cttcgggtaa agcatcggcc ctaaaccttc cagtaccaaa accagggcaa gcgccagcca
     5641 gattgtcgaa ttcattcatt atccttatag aaaaagaaaa ccaccgacat ccctgaggat
     5701 gcggtggctt tattgacctg taccgcagtc gttatattaa cgcgttgcgg aagtcggcgt
     5761 cttcatgtag cggaagaaat cgctatccgg gctcatgacc atcacgtcct gattgccaga
     5821 gaagctgttc tcataagcac gcaggctacg gatgaatgcg tagaagtccg gatctttact
     5881 gaatgcatca gcaaacagtt tggctgcttc ggcatcgcct tcaccacgca taatgcggcc
     5941 ctgacgctca gcttctgcca gcgttctggt cacttcatag tcggcagtcg cgcgcagttt
     6001 ttccgcttct tcctgacctt gtgaacggtg acgacgcgct accgcttcac gctcggcgcg
     6061 catacggttg tagatcgctt cagacacttc ggtcggcagg ttgatctgct tgatacgcac
     6121 atcgacaact tcaataccca gcgccgccat actgttcggg ttgatgaccg gaactttgcc
     6181 cttcgtctca gccgttacgc gctctgccgc ttcggcaatg gcgttatctg ctgccggggt
     6241 agtaacttca tcttctgtac ccgcagaacc ggagttcagc gcgtcacgta cttcgagggt
     6301 cagacgacca cgggaatcgg tgacgatatc tttcacgtcc aggcgaccaa tttcagaacg
     6361 cagacggtca gagaacttac gtttcaacag cacttccgct tgcgaaatgt cgccaccacc
     6421 cgttgccagg tagtaacggc tgaaatcgct gatgcaccat ttgatgtaag agtcgacgat
     6481 caggtctttc ttctctttgg tcacaaagcg gtcggcctgg ttgtccatgg tctgaatacg
     6541 tgcgtcgagc attttcaccg tttcaatgaa cggtatcttg aaatgcagac caggctcata
     6601 aaccaaaggt ttgttgtcat cgtcacgcag taccttacca aaacgcagcg taataccgcg
     6661 ctcaccttct ttgacgacaa agacagacat gtaaagcact accagcacga tgatgataat
     6721 cgcgataact gacttacgca tcgttattcc ccctgacgct ggtagtcgtt acgctgcgcg
     6781 ttggcgcggc gttggtccat aatatcgccc tgactggtgg acgacgtgtt gcttgctcca
     6841 ctggttgtgg aggaagaggc tggcggcaga cgcagcagat tgctggcacc gttatcgctc
     6901 ttcgccgcag gggcgttacc acctttcagc atctggtcta acggcagaac catcaggttg
     6961 ccacctttat cgttaaccag cactttgcgg gtgttaccca acactttttc catcgtctcg
     7021 atatacagac gctcgcgagt aatttccggc gcggctttat attccggcag aagtttagca
     7081 aagcgcgcca cttcaccctg agcttccagg atggtctggg ccttgtacgc acgcgcctct
     7141 tcgaggatac gttgcgcctg accgttcgca cgcggctgaa cttcgttggt atacgcttct
     7201 gcttcacgaa tgtattgctg ttcgttttca cgcgcggcaa tcgcatcgtc aaacgccgct
     7261 tttacttctt ccggcggacg agcagcctgg aagttgacgt ccagcagcgt gatacccatg
     7321 tcatacggac gaatcgtctc ttccagttcg cgctgagtat cgctacgaat cacggtacga
     7381 ccttccgtca gaatgcggtc catggtgtat ttaccgataa ctccacgcag ggcgctgtcg
     7441 gtagcctgac gcaggctgtc atccgggctg gtcacgctat acagatattt ttccggattg
     7501 gtgacgcggt actgcacgtt catctcaacg cgcactacgt tctcgtccga cgtcagcatc
     7561 acaccagaag cggccagttc acgcacggct tccacgttca ccggtttgac ttcgtcgata
     7621 aacgtcggtt tccagttcag acccggctca accagatggc tgaatttacc aaagcgtgtt
     7681 accacgccgc gttcggcttc tttaatggta tagaaaccac tggccgccca gataatgaca
     7741 atcgctgccg ctgcgatggt aacgacacga ccgccaagct gcgggcgcgg gccttgcgat
     7801 gaactgccac cgccagatcc ggtgccttta ccgccgccca gaccaccgag ctttttgctc
     7861 agtttgcgga agatatcatc taaatcaggt ggcccttgat cgcgaccgcc tttgtttcca
     7921 tttccctcag agttgccgcc aggtttgctg cttccccacg ggtcgcggtc ttgtccgtta
     7981 ttaccgggct gattccacgc catgtttgtg ctccatattt gttatgcggt gatccctgtt
     8041 gtctttcgac gccagaggat atgactccac gcttcagacg ctacgccgtt agatcaggta
     8101 atcgatcaac gccggttctt gtttacagag gcgacgccag tcaacgatcg gcatacgaac
     8161 ttgcagactt acgctgccgt cctcctccat ccactctttt tctattgcct gaagctgata
     8221 aaaacgactt ctcagacgcc cttcctgcgg tggcagacgc aatgtatgct gcgccacctc
     8281 gccggaaagc cgctccgtca aagcctgaaa aagctgtggt atccccgctc cggtctgtgc
     8341 ggaaagccag acacggatcg gtttgttctc ttcgtcccga tcaatacgcg gttcgaaatc
     8401 ttccagcata tcgatcttgt tcatcaccag cagggttggg atctcgtgag cgtcgatctc
     8461 ttcaagaacc gtattcaccg cttcgatgtt ttcttgtaca cgcacatccg ccgcatcaat
     8521 gacgtgcagc agtaatgtgg cttgccgcgt ctcttgtaac gtggctttaa atgccgccac
     8581 cagatcgtgc ggcaggtggc gaataaaccc tacggtatct gcaagtacgg tttcaccgac
     8641 atctgcaacg tcaatacgcc gcaacgtcgg gtcgagggtg gcaaacaact ggtctgccgc
     8701 gtagacccgc gcttcggtga tgcgattgaa aagggtagat ttaccggcgt tggtatatcc
     8761 caccagcgaa acagtaggaa cgtcggcttt gatacgcgat tgccgcccct gctcacgctg
     8821 cttttcaact ctttccaggc gcgactgtat ctgcacgatg cgattacgca acaaacgacg
     8881 gtcggtttcg agctgggttt cacccggacc acgcaaacct atcccgcctt tctgtctttc
     8941 aaggtgggtc cagccacgca ccaggcgcgt agccagatgg cgcagctgcg ccagctcaac
     9001 ctgcaactta ccctcatggg tacgcgcacg ttgggcgaaa atatctaaaa taaggccggt
     9061 gcggtcgata acacgacact cgcacaaacg ctccaggtta cgctcttgcg ccgtgctcag
     9121 ggcatggtca aaaagaacga ccgaagcacc cgtcgctttg acagcttccg caatttcaac
     9181 tgctttacct tcacctacaa aatactttgg gtgcggcgct ttacggctac cggtaatcac
     9241 ctgcaatgct tcgacaccgg cggaagagac cagagattca aactcctgga ggtcttccat
     9301 atctttgtct tgcgtaaaat agatgtgtac cagtaccgcc tgctcaccag catcataacg
     9361 gtcaaacaag cgtataaccc tctaaataga tcagcgggga acgcaggatc gctggctccc
     9421 cgtgtaaaaa aacagcccga aaccttattc ggtttcttcg ctgtcctgtt gcgcggaagt
     9481 attctgcgcg ctgctaccat gatggtagtt actgctggta ccgccaccgg cgttgttact
     9541 gtgatgagaa accgggcgag acgggacaac agtagaaatc gcgtgcttgt aaaccatctg
     9601 gctgaccgtg tttttcaaca ggatcacgaa ctgatcaaaa gactcgattt gcccttgcag
     9661 cttaatacca ttcaccaaat aaatagaaac tggaacacgt tcccgacgca gtgcgttcag
     9721 gaacggatct tgtaaagatt gccccttagc cattctctct tttccttata tgcttatttg
     9781 tactttgaac ctttcgattc tgaaaaaatt gcgcacgata cgtctcaatt gtacacattc
     9841 agcctgcgat agcaccaaca acctgtaata cttcgtcacg cgcctgttct ggtttttcac
     9901 tgtcaagcca gtgaacccct tcccaaccac gcagccaggt tatctgccgc ttcgccaact
     9961 gtctcgtggc gcaaacacct cgataaacca tttcatcgta tgagatttcg ccttcaaggt
    10021 aagaccacat ctggcgataa cccacgcaac gaatggaagg caagtccgta tgcaaatctc
    10081 ctcgggcaaa aagcgcccgg acttctgctt caaaacctga agccaacatc tgatgaaaac
    10141 gctgctcaat gcgttgatgg agcagttcac ggctcgccgg ggcgatggcg aactgatgca
    10201 cctgatacgg tagagcgtct cctgacgttt gcgtcagttc cgttaaagtt ttacccgaaa
    10261 tgaaaaaaac ttccagtgcc cgggaaagcc tttgtggatc atttggatga atccttgctg
    10321 ccgcaaccgg atctacctcc tgaagttgac gatgcaatga ctcccaacct tgctctgccg
    10381 cctgttgctc aattctggcc cgcacttccg ggtctgccga cggtagcggc gacaaccctt
    10441 ccagcaatgc cttgaaatac aacatcgtac cgcccactaa cagtgggatc cgccccgccg
    10501 cggtgatatc ggccatttcc gccagcgcat cgcggcgaaa atcagcagcc gagtaagcct
    10561 gcgacggatc gcgaatatcc agcaatcggt gcggcgcggc gagtaactct tcagcgttcg
    10621 gcttcgccgt cccgatatcc atccctttgt aaataagggc agaatcaacg cttatcaact
    10681 ctactggtaa aattttacgc agctcaatgg ctaacgccgt tttaccggag gccgtcggcc
    10741 ccatcaaaaa aatcgcctta ggcaggctcg ccttactgat atcactcatc tttcagggct
    10801 tttatcgccg gatgtaaatc aacagattgt aacagaccac ccggcggcgt tttcacaagt
    10861 tgcggacata accgttccac gtccgccagc agggttatgg cctgtgccat tgaccactgc
    10921 gcatgttcgc tcatcagatt tcgtgcaatc cactgcgcaa tattgccagg ttcgaatacg
    10981 gactgcttcg ccaggtagcc tatcagttca ggaatcaaga tttgtaaatt ttgttggcgt
    11041 aagggtaaag gcacggccct gatggtcaca tgctgtgcat ctgactggaa atcaataccc
    11101 aattccgcta gggcagactg cgctttttct aatgccgatt tttcttcgcc agaaactttt
    11161 agccgcaacg gaatcagcag cggctgggcg caaacgggcg cttcacccgg cgtcaattgt
    11221 gcctgacgca gccaacgttc tgccactggc aaggataaaa gtgaaatgtt gccatcgcgc
    11281 tccagcaacg cacagtcgga atggacgata gtcagtaccc gaccaaaact ctgactgttc
    11341 gccgcaagtg caggttcctg cggttccggc gcttttggtt tttgcatcgg cgcgggcgtt
    11401 tgcaaaagct ggcgatacac ttcaccttgc tgtttctggt agcctggctg cgcattcggc
    11461 cagggggcag ccggacgact gcctgaggcg ggcgcaggag agtagcgcgg agctaccggc
    11521 tcacgagctg ccggttctgc aaagtgattg cgccccgccg ccacgcggtt ttccgggatg
    11581 gcacgcggtg cgggttgtgg ttcatcgtcc agcggtagcg gcgtttccag ttgctgttgt
    11641 agcacgctca gcacgccctg atagataaaa tcatgcacca gacgcgactg atggaaacgc
    11701 acttcgtgtt tggcggggtg tacgttgacg tccacctgat gcggggcgat ctccagatac
    11761 aacacaaatg ccggttgctg atcggccccc agtttgtctt cgcaggcctg gcggatcgca
    11821 tgattgatca ggcgatcgcg catcatgcga ccattcacgt agcaatactg aatttccgcc
    11881 agtgcgggcg tagtgtgatt tggatcggcc acccagccgc gcagggtgag atcgccgtgt
    11941 tgccattcaa tcgccagcgc ttgttcgaga aaagcggtgc cgcaaatcgc gcctaagcgc
    12001 cgttcttttt gcccgccttc cggcactgcg cggtactgac gcacaatttt accgttatgc
    12061 gacaggttga tcgtgacgtc gaaacgcgcc agcgcaatgc ggcggatgat ttcatcaata
    12121 tggctaaatt cggttttctc ggtgcgcagg aatttgcgcc gcgccggggt gttgtagaac
    12181 agatccagca cctccagcgt cgttcccacc ggatgcgccg ccggtttaac cgtcacgtcc
    12241 atgtcgcgtc cttcggcata ggcctgccag gcttcctgct gttctgcggt gcgtgaagtg
    12301 agcgtcaggc gggaaaccga actgatactc gccagcgcct caccgcgaaa gcccaggctg
    12361 ataatggctt cgagatcgtc cagagaggcg attttactgg tggcatgacg agccagcgcc
    12421 agcgccagct catctttttt gataccgcag ccgttatcac gaatgcggat aagtttcgcc
    12481 ccaccgcgtt cgatatcaat atcgatacgc gtcgcacctg catcgaggct gttttccact
    12541 agttctttga ctaccgacgc aggtcgctcg accacctcac ctgcggcaat ctggttcgcc
    12601 agttgtggcg gtaagacctg aattggcatc aatcgtcctt agtttggcag cgtgcgatct
    12661 ggcgtcgtca ccgtactggc agtttgtgcc gttgcaccct gcggcgcaga ttgcatcgga
    12721 tgcgcaagga agtaattgcg caggcctttg taaatggctt ctgccagctg ttcttggtaa
    12781 tcgtcgctcg ccagcaaacg ttcttcgctg ttgttgctga taaaaccggt ttcgaccagt
    12841 actgatggga tatccggcga gcgcagaacg ccaaggctgg cgtgttctgg tcgacgttta
    12901 tgtatttcgc caatgcgttg caactgactg atcatactgg tcgctacatc ataccctacc
    12961 cgctgggaat gaccgaactg taaatccagc accgcctggc ttaaataggg gtcagactga
    13021 ctgttcgcca gcacatcacc cgccccaccc agcagctccg actgtttctc gtgctgctcc
    13081 agccagctgg ccatttcact gttggcgcga cggttagaga gcacccatac ggaagcgcca
    13141 gtcgcactgc ggttcggtgc ggcatcagcg tgaatcgaca cgaggaaatt ggcgttttgc
    13201 ttacgtgcca catcactgcg ccccatcacc gagataaagt aatccccgtc acgggttaaa
    13261 acgcctttaa acatcggatc gtcattgagc aaagtacgca atttacgcgc aatggcgatg
    13321 gtgacatttt tctcccgcgt accaccgggg ccgatagcgc cagggtcctg accgccgtgt
    13381 ccggcatcaa tagcgatgat aattttatcg ccagtgttag ccgtcgcgcg cgctgccgga
    13441 cgcgttaccg tattactgct gataacaccc gtagtgcggt tactttccgt tttaaacgga
    13501 ttgcgcgccg gttcgctgac gcgcggtgcg acaaccgcag gcgtttcaac gcgtttcgca
    13561 accacgggcg gcggaggagg cggtggcggc acatcggcgt taatcgtaaa gacgacagtg
    13621 taattgctgc cattctgccg cttcaccgct tcggttttac cgttttcggt aagatcgacc
    13681 accagccgta gcgtttgtgc atctttaggc gttccagagc gaatcgcctt caccagatta
    13741 ttgccgctga acaacaacgg cagtccctga atcacgcccg tttgtttgat atcgagcgcc
    13801 acggtgcgtt tgctttgatg gctaaacgca taatcaggat cgccaataaa actcaacgtt
    13861 atccgcgcct gttgattacc gttagaaacc tgaatatcag agagcgtcgc ggcacccacc
    13921 ggcgtgcaca gcagcagcag cgtcgctacc aaccaatttc tgatgcgata catcatcccg
    13981 ccacctttca aaggttaacc ggctaaacgc gccagcaaca attcacccgc agaggaaacc
    14041 gcactcacgc gcgcctcacg gccttgtgcc tgataatcaa tgtgtatttc gacatccggg
    14101 tcaggaagaa cacctgtacc ttgttgtggc cactccacca ggcagatggc atcgttggca
    14161 aaataatcgc ggatccccat aaactccagc tcctcgggat cggcaaggcg gtacaaatca
    14221 aagtgataga ccattaagtt gtcgagcgta tagggttcga ccagcgtata agtggggctt
    14281 ttgacattac cctgatgacc cagagcctgt aaaaagcccc ggctaaaggt ggttttacct
    14341 gcgcctaaat cgccatacag atagattacg gttgcgccat cgcaggcttt cgctacccgc
    14401 tcgcccaggt ctaatgttac ctgctcatca gggagcggaa ttactcgatt catcatggtt
    14461 tttatcagtc acttccgggt taacaatacg ctgtagcgtg gaaaagagat cggttgccag
    14521 catcccgcgc gttccaaaac gcgccgccag tacgtcagct gccgcaccgt gcgcgacaca
    14581 gcctgcacag gctgcatcat acggcgacag tttttgccca agcaatgcgc caataatacc
    14641 agagagcaca tcgcccatgc cgccgctcgc catgcctgca tttccggcat caataatgcc
    14701 taaagcgtca ggatgggcgg cgaccacggt tccggcacct ttcagcaccg ctacgccgcc
    14761 ataacgttgt accagacgtt tggcgcaatg taagcggtca ctttcaattt cagcgacgga
    14821 acagcctaac aaccgtgcgg cctcgccagg atgcggcgtg atcacgcgat tgtgacgctt
    14881 atcgggattg attgccagca ggttcaatgc atcggcatcc cacaacatcg gtttgcgaaa
    14941 attctcaact ttttgcaggg cttttttccc ccactcttgc tggcccagac cgggaccaat
    15001 caccaccaca tcggcccatt ccaggctttc ggtaagagag tccatagtca gttcatgcac
    15061 catcaattct ggtcgtgcag tcagcagcgg cgcaatgttc tcactgcggg tcagtactcg
    15121 gactaaacca gcaccagcac gtagcgccgc ttcccccgtc atacgaatag cccccgccgt
    15181 gccgtgatcg ccaccgataa tcaccagccg cccgtgatcg cctttatgcg aagtcgggcg
    15241 acgcggtttc agccagtgag aaagttgttc tgccgaaaac cgctgaattt tcgtctcctg
    15301 acctgccagc cagctatcca gccccagtga gtcaaaatgc agttgtcctg taacatcccg
    15361 cgcttttcca gtgagcaagc ctagtttcag cgcaataaaa gtgatggtgt gatcggcgtt
    15421 gatcactgcg cctggcgtag cgccggtttc agccagcagg ccggaaggga tatcaaccgc
    15481 cacaatcggc gcaggatggg aattagcgtg gtcgattaac tggctaatgg attcgcgggg
    15541 cgcttgctgc aagccggtac cgagcagcgc atcaacaatc agatctaccg attcgggcca
    15601 gacaatattc gaagcatgga tctcgccacc cgcgtttaac catgcttcgc gtgccagcgc
    15661 ggcctcttcc ggcaacggtt tgtcgctctc ctgggccaac aacgtgacct caatgccgac
    15721 cgctttggcc agtcgcgcga ccacgtagcc atcgccgccg ttattaccat gaccgcacag
    15781 caccagccag tggcgggcgt caggatacgc cgaacgacac acctggaatg cggcctcgcc
    15841 agcgcgaagc atcagctcat agagtgtgag ccccagcaca tctgccgcct cgcgttctcc
    15901 gcggcggata tcgtcggcgt accagacggt gtgtggtata cttacggggt ttttcttcat
    15961 tgtatggtcc gtcatgtcag agcccctcga tctcaatcag ttagcgcaaa aaattaaaca
    16021 gtgggggctg gaactgggct ttcagcaggt aggtattacc gataccgatc tcagcgagtc
    16081 cgagcccaaa ctgcaagcat ggctggacaa acaataccac ggcgaaatgg actggatggc
    16141 acgtcacggt atgctgcgcg ctcgccccca tgagttatta cccggtacgc tgcgtgtaat
    16201 cagcgtgcgg atgaattacc ttcctgctaa cgccgcattt gccagcacgc tgaaaaaccc
    16261 caaactcggc tatgttagcc gttatgcgct gggccgtgac tatcacaaac ttctgcgcaa
    16321 ccgactcaaa aagctgggcg agatgattca gcaacattgt gtttcgctga attttagacc
    16381 gtttgtcgat tctgcgccta ttctcgagcg cccgttagct gaaaaagctg ggctcggctg
    16441 gacaggtaag cactcactta tcctcaatcg cgaggccggt tcgttcttct ttttaggtga
    16501 attgctggtc gatattccgc tgcccgtgga tcaaccagtc gaggaaggat gcggcaaatg
    16561 cgtggcctgt atgacgattt gcccgaccgg tgccatcgtc gagccatata ccgtcgatgc
    16621 tcgccgctgt atctcttatc tcaccatcga acttgaaggg gcgatcccgg aagagttgcg
    16681 accgttaatg ggaaaccgta tttacggttg cgatgactgc cagcttatct gcccgtggaa
    16741 tcgctattcg caactcacta cagaagacga tttcagcccg cgtaagccgc tacacgcacc
    16801 ggaactcatt gagttatttg cctggagcga agagaagttt ttaaaagtca cggaaggatc
    16861 ggcgattcgc cgtataggtc acctgcgctg gctgcgtaat atcgccgtgg cattaggcaa
    16921 tgccccttgg gatgaaacga ttttgacagc gctggaaagt cgtaaaggtg agcacccact
    16981 tctcgatgag cacatagcgt gggcgattgc gcagcaaata gagagacgaa atgcgtgcat
    17041 agtcgaagtg caattgccga aaaaacagcg tctggttcgg gtgattgaaa aagggttacc
    17101 gcgtgacgcc tgattcatcc acagcttgtg aataaaaata aaaacgcatt gcaattcaag
    17161 acgaataaaa tcgtcaagtg atcgactcga caaaacggaa tcttgatttt taacttattt
    17221 atcaataggt ataaaaaata ccattcaccg tgcgaagttt ctttgaaatt aagattacag
    17281 cctgaggctg tggataactc tgtttacaag agtttgtcag agacaacaaa aaccagcaat
    17341 aacgcatcgc gtcgctgtgg atattttatt gagagaaga
//
LOCUS       DBKEKI010000077        17307 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.077, whole genome shotgun sequence.
ACCESSION   DBKEKI010000077 DBKEKI010000000
VERSION     DBKEKI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.077"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(31..2478)
                     /gene="glgP"
                     /locus_tag="ACUQPX_002588"
     CDS             complement(31..2478)
                     /gene="glgP"
                     /locus_tag="ACUQPX_002588"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HHV9057266.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQVIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="ACUQPX_002589"
     CDS             complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="ACUQPX_002589"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HHV9057267.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRALRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="ACUQPX_002590"
     CDS             complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="ACUQPX_002590"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HHV9057268.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="ACUQPX_002591"
     CDS             complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="ACUQPX_002591"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120549 - limit dextrin
                     alpha-1,6-maltotetraose-hydrolase activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HHV9057269.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="ACUQPX_002592"
     CDS             complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="ACUQPX_002592"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HHV9057270.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDNLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9672..10775)
                     /gene="asd"
                     /locus_tag="ACUQPX_002593"
     CDS             complement(9672..10775)
                     /gene="asd"
                     /locus_tag="ACUQPX_002593"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HHV9057271.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10967..11560
                     /gene="yhgN"
                     /locus_tag="ACUQPX_002594"
     CDS             10967..11560
                     /gene="yhgN"
                     /locus_tag="ACUQPX_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HHV9057272.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="ACUQPX_002595"
     CDS             complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="ACUQPX_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HHV9057273.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVL
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="ACUQPX_002596"
     CDS             complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="ACUQPX_002596"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HHV9057274.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="ACUQPX_002597"
     CDS             complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="ACUQPX_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HHV9057275.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14846..15541)
                     /gene="yhhW"
                     /locus_tag="ACUQPX_002598"
     CDS             complement(14846..15541)
                     /gene="yhhW"
                     /locus_tag="ACUQPX_002598"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HHV9057276.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPYKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15664..16701)
                     /locus_tag="ACUQPX_002599"
     CDS             complement(15664..16701)
                     /locus_tag="ACUQPX_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HHV9057277.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17034..>17307
                     /locus_tag="ACUQPX_002600"
     CDS             17034..>17307
                     /locus_tag="ACUQPX_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="YhhY; regulated by the fur regulator; unknown
                     function; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HHV9057278.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWK"
BASE COUNT         4251 a         4743 c         4313 g         4000 t
ORIGIN      
        1 cctttggccc cgttctattt attagtgaaa ttacaatctc accggatcga tatgccagat
       61 atgatcagcg tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt
      121 cagcatcgct tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg
      181 acaatcgaca tagctgcgat aatccgccag tacctggtag tgatcgccga agttgatcag
      241 cgaatcaacc agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat
      301 ttgcgtcagc acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc
      361 ctgacgacgc agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc
      421 accgacatga tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc
      481 gttaagcgca aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg
      541 ttcagacaga tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat
      601 gaacacgact ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc
      661 attgatcaaa tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa
      721 aatattcacg cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt
      781 aatcacatgc aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac
      841 atcgaacaac gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag
      901 acgctttttg ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca
      961 gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc
     1021 gtccagcacg gctgaaagcg atgggttcgc taccgccagc cagcgacgcg gcgtcacacc
     1081 gttggtgacg ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg
     1141 caccatcaga ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc
     1201 cagccaggcc atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag
     1261 cagatcggta tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc
     1321 aaagatgacc tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc
     1381 cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa
     1441 cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga
     1501 cagtaccgga tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt
     1561 tttatgcaac tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccaggaagta
     1621 ttcctgacgc aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg
     1681 agatacgttc tcggagtggt ttttatcttc cactgccgcg aagtagtcac cctggttgaa
     1741 tttaccgagg ttaatttcgc tactggcttg cgcactccac aaacgcagcg tgttggtcgc
     1801 gtcggtgtcg taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc
     1861 aatccagcgc gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg
     1921 cgtgttgtgg cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc
     1981 tttctggcta ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta
     2041 accgcgcccc ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc
     2101 caggccaccg ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa
     2161 ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt
     2221 ggagagcgta cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg
     2281 cgacaactgg gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa
     2341 taacgttgcg ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa
     2401 catcagcttg taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata
     2461 tgtaaacgga gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc
     2521 acggtacgac ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac
     2581 aaaccgccac agtgaaggac gggaccacag tacaaaagca cgccgaagag cccgtaacag
     2641 cgaccaggca ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc
     2701 aagagaacag tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt
     2761 accgtacttc aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag
     2821 aatgacgtcc gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac
     2881 gcccacctga ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc
     2941 gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga agccccggta aggcttccag
     3001 caccaggtcg agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt
     3061 atcgtcaacc ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc
     3121 caacgtatcg cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt
     3181 ctcgtccacg ccgttcagta cgcctgaaag acgcccttca cggtgacgct gttgcaacag
     3241 accttccata ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac
     3301 cgccgtaatg tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa
     3361 ttccagccca tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc
     3421 ataaaacatg ccttgatagg ccaggttgtg cacagtaaac accgacttcg caggacgccc
     3481 gcgcgccgcc agatacgcag gcgcaagacc tgcatgccag tcgtgcgcat gcaccacatc
     3541 aggacgccag aatgggtcaa gcccgctggc catttctgca ccaacccacc ccagcagcgc
     3601 aaaacgcaat acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg
     3661 acgatcatag agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa
     3721 caacagcgtg atatgcccgg cgaaagtatc acgacgggat actacctgcg catcggtcac
     3781 gccacggcga atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat
     3841 ttgtgctgcg ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa
     3901 catctctgaa catacatgta aaacctgcat tatcgctcct gtttatgccc taacttccgt
     3961 agcatttcgc gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct
     4021 tcctctgcgt tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg
     4081 cagcggcgca gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca
     4141 atgttgcaga atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa
     4201 ccggagatca cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag
     4261 cgatcctgca cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg
     4321 cgatcgtaca tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa
     4381 gcttccagcg tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa
     4441 gagagcggga acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct
     4501 ttgccaaagt cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga
     4561 tagtcggcgt caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc
     4621 attgacggtg ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc
     4681 gccataacgc caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa
     4741 cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg
     4801 tcgcccgcca ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg
     4861 gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc
     4921 agcagatcga caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc
     4981 tgcaccagag tgtgggactg gtactgggtg atcacgccca tacgacggat cccagagttg
     5041 atgcagttag acagcgcaaa gtcaataatg cggaacttac cgccgaagtg tacggccggt
     5101 tttgctcgct tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg
     5161 gcaacagatt tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta
     5221 accatgacta actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct
     5281 gccagacaac cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact
     5341 ccccagcagg taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc
     5401 gatccgagag cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag
     5461 gttgagcata tcgatttagc caacggacat tgccgtcacc ttcttcccac cagcgattct
     5521 ccaccaaagc gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac
     5581 cactgcttgc ctgcgaccag tccaaccagt ttaattggtt atcctgacag taggcattgt
     5641 tattgccatg ctggctgtga ccatgttcgt caccggccag taacatcggc gtgccctggg
     5701 agagcaacaa cgttgttaac agggcatgaa tgctgtcgcg ccgccgttca accaggtcaa
     5761 gagtaccgcc taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc
     5821 gattttcttc tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg
     5881 taaaaccgtc atgcgcggtg acgagattaa tcgcggcact cggcagacga ccattacgtt
     5941 taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat
     6001 gtagccagaa acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg
     6061 ggaaatttcc cacctgataa ccactaggag cgatatccca cggttcagca attaacttca
     6121 cctgcgagag caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact
     6181 ctggcgtacg gcccatgact gccgccagat caaagcggaa accatcgacg tggcaggttt
     6241 ctacccaata acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga
     6301 gcgtgttgcc gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc
     6361 tacggttatc gatcccacgc agcgagaata acgggccgtc gaggtccagt tccgcactat
     6421 ggttgagcac gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat
     6481 cgcgaaactc atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca
     6541 tcgccaccgg gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac
     6601 tggcaaactg cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt
     6661 tgatcatcac cggatgcccg agggctttat aagtgccacg gatctcgacc gggatctccg
     6721 ggtgcaagta cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg
     6781 tgcgcggcgg ggcgtcatct tcccagtcat agtgatcaac caccactacg catttcggcg
     6841 caatggcggc gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat
     6901 ctttaaactc cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa
     6961 agcgatgccc ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg
     7021 ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact gttccctggc aagtcatagc
     7081 gatgttcctg gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga
     7141 aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa
     7201 tggcgagttg tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagcgt
     7261 caggcttagt gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc
     7321 attgcctgca ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt
     7381 gcctggctga ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc
     7441 aacgataatt tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt
     7501 gtcatccacc accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt
     7561 atggtggcgg taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt
     7621 atcgccgcct tccaacagat gccagtcgag gctggcgtca tggttccact cgcggccctg
     7681 ggcaaattcg ttccccatga acagcagttt cttgcccgga aatgcccaca tccagccata
     7741 gtaagcgcgc aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga
     7801 ttttttaccg tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt
     7861 gtagagcatc ccgaaggtca ggttatcgtg atgatactga cgataaatcg ggtcgagctt
     7921 catgtagtcc agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc
     7981 gcccatatcc tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac
     8041 cgcaccggaa acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc
     8101 aagattctcg cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc
     8161 acgataaatc atcgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat
     8221 ccagtaaagc gcgttaccga cgaggaagtt actgacttca cggcgaccat agttgtagat
     8281 cagcgtgttc cagtcctgat gatagccttc acgcggatcg ctgtgttcat acaagttcgt
     8341 gccatcaaat tcggcaagcg caaagtcatc agtcgggaag tggcctggca cccagtcgag
     8401 aatcacgttc agaccagctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt
     8461 accaaaacgg cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa
     8521 gggatgctcg ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg
     8581 caccagttga tcggccagct cgcggtagct caaccagaaa ttgttgtcgg tgtggcgacg
     8641 ccaggaaccc aggtgaactt cataaataga gattggcgca tcaaactgat tcgctttttt
     8701 gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg
     8761 gcgcatttgc gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc
     8821 aatcatctcg tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccagat
     8881 gccgctctct ttacgcaggc gcatcgggtg acggcgaccg tcccagtagt tgaattgccc
     8941 aaccaccgag acccgacggg cgtttggagc ccagacagag aaacgcgtac ctgtgacgcc
     9001 atccatagta tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga
     9061 taatagccag gcatccattt cctggattag cggaccaaaa cggtaaggat catcaatcag
     9121 gttttgctga ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg
     9181 aatgacgccg ctaaagaatc cccgtgagtc gagacactcc agttttgcga gtttgcgccc
     9241 ggttttcggt tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag
     9301 tcccgcggtg gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat
     9361 tagcgcgtta atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg
     9421 tgtcacccca tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg
     9481 cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc
     9541 atgtattcag ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga
     9601 aacatctgcg cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta
     9661 atgaataaag attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac
     9721 agcagctggt cgcccacggt aaaggctgac aggaactctg gtcccatatt cagcttacgc
     9781 aggcggccta ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata
     9841 gtgatttccc gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct
     9901 tcgacggtcg gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag
     9961 cgcaatgccc cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg
    10021 atcttgttgg tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt
    10081 ttgtcgatcc acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc
    10141 tcaccgctac gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg
    10201 gtcgcgagtt catctgccac atggccatac agatggccca tctgggttaa taactcacgc
    10261 atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca
    10321 agatcattgg cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg
    10381 ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg
    10441 tcaagaatga tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa
    10501 ccttgccatc cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga
    10561 caggtcacaa tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg
    10621 ccagtggttc cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca
    10681 gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc
    10741 ataccgcgcc agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg
    10801 tgctgtataa atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa
    10861 agtcgcaagt gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt
    10921 tgttagcacg cagaaagccc gcggcaatta tcagggaatt tgagttatga atgaaatcat
    10981 ttctgcagca gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat
    11041 gtccgtactg aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct
    11101 tattgctctc ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct
    11161 cagcctgcga gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat
    11221 taagatgatt ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc
    11281 atttatcgtg ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat
    11341 gttgttgtct catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc
    11401 ctggggcggc acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga
    11461 gaaaggggtg aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaactca
    11521 gatgttcctc gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa
    11581 gagcccggta gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat
    11641 catcccaacg atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag
    11701 cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccag cgtcgttaac
    11761 gtggctgaca acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc
    11821 agagtagttc agttgttcaa tgactggcat caccagtccc accgccgtta aacaggctac
    11881 ggtggcagac ccctgaatga tgcgcactgc agcagccagc acgaagcagg tgatagcaat
    11941 cggcaggccc atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag
    12001 cacctgtttg aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgcag
    12061 cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc
    12121 ataaatcgct accagacaag caaccagaat cgcggtaaac ggatgaccaa taaactcgaa
    12181 ccattcgtaa gcggtagagc cttctggcac aaaacgcgcg gcaatggttt tcagccccac
    12241 cagcaccagc ggcaacagga tcagcgacag gctgaatccg aaagatggca ttttaccttc
    12301 gccgagatgc ggttcgctga tgtcgtcagg aatatgcagc tcaacgtaac ggctgatgaa
    12361 gttaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca ggccaatcag
    12421 gatcatccag ccaaagtcgg cattcatctg cgatgccagc agcattggcg ctggcccaag
    12481 caccaggaac gctgccgcgg ccgccacgcc tgcgaataat gggattacca gcttcaccag
    12541 gttcgtaccg gtgtgacgcg ccattgagaa agcaacgcta atcagcagaa caatcgccac
    12601 ttcaaagaac agcggtagcg cacagaccag ccccgcaagg ccgatggcat aatgcgcgcg
    12661 gctgtgaccg aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcata
    12721 taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggtgcc
    12781 tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc
    12841 agcccccatg gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa
    12901 aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac
    12961 ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc
    13021 gatatccacc accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg
    13081 cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaggc gactttcaat
    13141 cacatcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa
    13201 gtcgcgatag tgctttttca gtgcagaaca gacgatcagc gacactttat tggtgcgctg
    13261 catagcaaac gcggagtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag
    13321 cggttcgcca gacgccattt tttcgatatt gcgccgtgga tggaggaaat cgccatcaag
    13381 aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga
    13441 tacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc
    13501 acaatacaag aattaaaaat gttacgggta actgttatcg gtaacattgt ccagccggac
    13561 aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt
    13621 gtgggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt
    13681 cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc
    13741 acgcggcgtc agcacgctcg caagtcgtgg ctccatcacc tgaccaatgt catgaccgtg
    13801 gaaaccggca atcgccatat cgtcaggaac ttttaacccc agacgctgac attcaaacgc
    13861 cgcgccgacc gccagatcat cattagtaca gaacacgcca tccagctgcg gatattcccg
    13921 ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac
    13981 gctatatggc accaggcctg catccagcat cgcctgttcg tatcccttct gtttgatgat
    14041 agtacgttcg tcgagacgtg cgccgagata ggcaatgtgg cgatgcccgc gagcaataat
    14101 ggcggtagtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca
    14161 cggcgactgg ctgtccatca actccaccac cgggatcccc gccacttcaa tcatctttaa
    14221 ggtgcgcggc gtgtgggtac gttcggtgag gatcaggccg tcgatattcc aggagagcat
    14281 ggattcgaga cgttcttgct ccatttccgg tttataaccg tagtgcgcca gcatggtctg
    14341 ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt
    14401 ggtgagagaa ggtaacagga cgccaatcgc ccggctggta gcgttagaga ggatatcggg
    14461 cgcacgattg ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc
    14521 gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac
    14581 acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa
    14641 gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct
    14701 tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc
    14761 aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg
    14821 caggcttcaa agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc
    14881 tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg
    14941 tcgaggcttt cacgccgtta atggtaacat tgccttttac cacctggatc cagacgcggc
    15001 gttcagcggc aatctgatgc accgactgct catctttcag caacgcccaa cgatacagtt
    15061 ccatatcctg atgcactttc agcgaaccat ctcgcgcatc cggcgagagc accagctgtt
    15121 tgccctgcac ggcatcgaag cgacgctgtt cataacgcgg cgtaatgcca ttttcttcag
    15181 gcatgatcca gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt
    15241 gacgaatacc cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat
    15301 tgcccatgct gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat
    15361 ctttatacgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc
    15421 gcagcgcgga gaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc
    15481 aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca
    15541 tttgtattcg ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat
    15601 gatagagggt gaaaattgac ccctctgttc aaaaaaattg aactattcga ggggccatca
    15661 aacttaattc gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat
    15721 ttccagattg gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt
    15781 ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct
    15841 gaccgtcaca ccctcgtcat tgacatactc cagcacaccg accgaatcat ccgctgcgaa
    15901 tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta
    15961 tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag
    16021 atggctggtt ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc
    16081 gtcaggattg gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc
    16141 gaacagagaa ataatctggt ccatcgtatg cacaccaagg ccatagaacg cgccatcctg
    16201 cggcagtcca ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc
    16261 gacaatctct cccagcttgc cgctttcaat cgcttttttc gccgtcagga agcaggaatc
    16321 aaagcgacga ttctgatatg gcgtgacggt cagccctttg cttttcgcca gtgcaaacag
    16381 ctctttcgcc tgcgcaagtg tcggagtgaa cgttttttcg accagcacat ttttcccggc
    16441 ttccagcgcg cgcttcgcgt actcgaagtg gctgtccgca tgggtgcaga caacaaccaa
    16501 cttaacatcg ggatcgttta gcacttcgtc gagatcgctg gtgaaatgga tatgagagta
    16561 aatgggagcc tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct
    16621 atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat
    16681 aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg
    16741 caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa
    16801 attgagaacg aaagatcaaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    16861 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgaaacaat
    16921 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc
    16981 atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
    17041 agatagtaat acgccacgca gaaacacggg attacgaggc catcaggcag attcacgccc
    17101 agccggaggt gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg
    17161 agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg
    17221 tgggccatct caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg
    17281 gtatctgtgt cgactctcgt tggaaga
//
LOCUS       DBKEKI010000078        17228 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.078, whole genome shotgun sequence.
ACCESSION   DBKEKI010000078 DBKEKI010000000
VERSION     DBKEKI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.078"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..158
                     /locus_tag="ACUQPX_002601"
     CDS             <1..158
                     /locus_tag="ACUQPX_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9057279.1"
                     /translation="DPPPFHEIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGR
                     GREWKDI"
     gene            394..1212
                     /gene="ybhA"
                     /locus_tag="ACUQPX_002602"
     CDS             394..1212
                     /gene="ybhA"
                     /locus_tag="ACUQPX_002602"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HHV9057280.1"
                     /translation="MTTRVIALDLDGTLLTLKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1213..2271)
                     /gene="modC"
                     /locus_tag="ACUQPX_002603"
     CDS             complement(1213..2271)
                     /gene="modC"
                     /locus_tag="ACUQPX_002603"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HHV9057281.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2274..2963)
                     /gene="modB"
                     /locus_tag="ACUQPX_002604"
     CDS             complement(2274..2963)
                     /gene="modB"
                     /locus_tag="ACUQPX_002604"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HHV9057282.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2963..3736)
                     /gene="modA"
                     /locus_tag="ACUQPX_002605"
     CDS             complement(2963..3736)
                     /gene="modA"
                     /locus_tag="ACUQPX_002605"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HHV9057283.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3903..4052)
                     /gene="acrZ"
                     /locus_tag="ACUQPX_002606"
     CDS             complement(3903..4052)
                     /gene="acrZ"
                     /locus_tag="ACUQPX_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HHV9057284.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4181..4969
                     /gene="modE"
                     /locus_tag="ACUQPX_002607"
     CDS             4181..4969
                     /gene="modE"
                     /locus_tag="ACUQPX_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HHV9057285.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5037..6509
                     /gene="modF"
                     /locus_tag="ACUQPX_002608"
     CDS             5037..6509
                     /gene="modF"
                     /locus_tag="ACUQPX_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HHV9057286.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQTLMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6770..7786
                     /gene="galE"
                     /locus_tag="ACUQPX_002609"
     CDS             6770..7786
                     /gene="galE"
                     /locus_tag="ACUQPX_002609"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HHV9057287.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7796..8842
                     /gene="galT"
                     /locus_tag="ACUQPX_002610"
     CDS             7796..8842
                     /gene="galT"
                     /locus_tag="ACUQPX_002610"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HHV9057288.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8846..9994
                     /gene="galK"
                     /locus_tag="ACUQPX_002611"
     CDS             8846..9994
                     /gene="galK"
                     /locus_tag="ACUQPX_002611"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HHV9057289.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGNKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9988..11028
                     /gene="galM"
                     /locus_tag="ACUQPX_002612"
     CDS             9988..11028
                     /gene="galM"
                     /locus_tag="ACUQPX_002612"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HHV9057290.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLSPGEE
                     YSSLTEYQFIAE"
     gene            11231..11983
                     /gene="gpmA"
                     /locus_tag="ACUQPX_002613"
     CDS             11231..11983
                     /gene="gpmA"
                     /locus_tag="ACUQPX_002613"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HHV9057291.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12150..13202)
                     /gene="aroG"
                     /locus_tag="ACUQPX_002614"
     CDS             complement(12150..13202)
                     /gene="aroG"
                     /locus_tag="ACUQPX_002614"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HHV9057292.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13518..13898
                     /locus_tag="ACUQPX_002615"
     CDS             13518..13898
                     /locus_tag="ACUQPX_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HHV9057293.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14012..14947
                     /gene="zitB"
                     /locus_tag="ACUQPX_002616"
     CDS             14012..14947
                     /gene="zitB"
                     /locus_tag="ACUQPX_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HHV9057294.1"
                     /translation="MAHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGSLA
                     LLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVITIL
                     IVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHVLG
                     DLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSL
                     DIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMD
                     HYQIEHATIQMEYQPCHGPDCHLNEGGSGHSHHHH"
     gene            complement(14944..15663)
                     /gene="pnuC"
                     /locus_tag="ACUQPX_002617"
     CDS             complement(14944..15663)
                     /gene="pnuC"
                     /locus_tag="ACUQPX_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HHV9057295.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15701..16744)
                     /gene="nadA"
                     /locus_tag="ACUQPX_002618"
     CDS             complement(15701..16744)
                     /gene="nadA"
                     /locus_tag="ACUQPX_002618"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HHV9057296.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFVAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(16993..17068)
                     /locus_tag="ACUQPX_002619"
     tRNA            complement(16993..17068)
                     /locus_tag="ACUQPX_002619"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17033..17035),aa:Lys,seq:ttt)
BASE COUNT         4344 a         4512 c         4292 g         4080 t
ORIGIN      
        1 gtgacccacc gccatttcat gaaatccgaa gtttatccgg tcgcctgcac tcagcagaaa
       61 aaggcagtga tttcactcag gcgctccttg gacatcgttc atccagcatg acggataaat
      121 accgtgacgg acgagggagg gaatggaaag acatttagaa ttaatttcga atgatttcga
      181 aatgccactt gtaattaatt gattataaag gattaattcc taaagagcat ttgcttcatg
      241 aatgctcctt tgcatttagc aactgactgt aacagctaaa attagtcgct tttggcggtg
      301 aatgctgaat taaaacgcag aattttgtcc gatagtggta tcggtgtacc attcgcgcag
      361 acgaaaattc ttaaccttaa tctggaatac gccatgacca cacgcgtgat tgctctcgac
      421 ttagacggca ccttattgac cctgaaaaag accttgcttc catcatcgat agaagccctg
      481 gcccgcgctc gcgaagcagg ctatcaatta atcatcgtca caggtcgcca tcacgtcgct
      541 attcatcctt tttatcaggc gctggcgctg gatacacctg ctatttgctg taatggcacc
      601 tatttgtatg attatcatgc aaaaaccgtg ctggaagcgg acccaatgcc cgttaataaa
      661 gccctgcaac tcattgagat gctgaatgaa caccacattc acggtctgat gtatgtcgat
      721 gatgcaatgg tctatgagca cccgaccggg catgtcattc gcacgtctaa ctgggcgcaa
      781 accctgccgc cggaacagcg tccgactttc acacaagtcg cttctctggc tgaaacggcg
      841 caacaagtta acgccgtatg gaagttcgcc ctgacgcacg atgacctgcc gcaattgcag
      901 cattttggta agcatgtcga acatgaactg ggactggagt gtgaatggtc ctggcacgat
      961 caggttgata ttgcacacgg cggcaacagc aaaggtaaac gtttgacgaa atgggttgag
     1021 gcgcaaggtt ggtcgatgga aaacgtcgtg gcattcggcg ataactttaa tgatatcagt
     1081 atgctggaag ccgctggtac aggcgtggcg atgggcaacg ccgatgacgc ggtaaaagcg
     1141 cgcgccaaca ttgtgattgg tgataacacc accgacagca ttgcccagtt catttatagc
     1201 cacctgattt aatcaggcgg ttatcgacac acttttaatt tgcgcgtaca gccacaggcc
     1261 aggtttgatc gccagttcat ccctggccca cgggctgata cgcgcccaca gcgttttacc
     1321 gccgacttcc agctccactt ccacctggcc gttgtcgtca taactattaa caacttttgc
     1381 ccgcaatacg ttacgaatgc tggtttgctg cggcggttgt aaaaccagag aaacatcgga
     1441 agcctgaatg cggatacgta gcgcagcttg cagcggttcg cccagcttat tgacccacaa
     1501 atgctgatcg cccagcgcca gcgcggtcat cgcgtaatgc ggatgatgct caagcaccgt
     1561 cactttcaga atgctacttt gttgctcttt cggcagccac ggattcatca cgctactgcc
     1621 ccacacttcc tccagcgcgc caaaggcttt cacctgaccg ttttccagta ccatcactct
     1681 gtctgccaga tggaggatct catccagcga atggctgaca tacaacatcg gaatgttgat
     1741 ttcccgtgtc agccgttgca gataaggcaa cagttcgcgt ttacgcggaa tatccagtga
     1801 cgccagcggt tcatccagca gcagcaattc cggtgctgtc agcaaagccc gaccaatcgc
     1861 cacgcgctgt ttttcgcctc cggacaggct gcctggtaaa cggtcaagca acggttcaat
     1921 gcctaaaagc gccaccagct tatcgaactg atcgaccata cttttcgaca tgccgtagcg
     1981 cagattgcca cgcactttgt aatgcgggaa cagccgcgca tcctgaaaaa catagccaac
     2041 gcgacgcttt tccggcgtca ggcagatacc tttttcggca tcatttagta cccgcccatt
     2101 gaggacaatc cgcccttttt gcgggcgcgt cagtccactg atggcgttaa tcagcgaagt
     2161 ttttccggca ccggagacgc caaagatagc agtgatgcca ttggcgggca gcgtttcatt
     2221 aatggtcagg caatggttgc ccaacgtctg ggaaaaattc agctccagca tgattagcgc
     2281 cccgcccgtt cacggctgat tctggccagc cattctgaaa tcaacaggga gatcatcgcc
     2341 agcgcaatag aaataatgca cagtctcgcc gcgccacttt ccccgccggg ggtctggatc
     2401 agggtataca tggcagaagg aatggttcgc gtttcgctag gaatgttcga cacaaaggtg
     2461 atggttgcac caaactcacc gagagaacga gcaaaagcca gtaccgtacc aacaataatt
     2521 cccggtaagg tcagcggtaa cgtgatagta aagaaaacgc gccagcgccc ggcacctagt
     2581 gttcttgcgg cctgttccag tttgacatca accccttcca gcgccagacg aattgcccgc
     2641 accatcagcg gaaacgacat gacggcggca gcgagaaccg cgccgcgcca gctaaaggcg
     2701 aaggtaatac caaaccagtc atacagacgt tcaccgataa atccgcgccg tcccatcgaa
     2761 actaataata agtaaccgac gaccacgggc ggtaacacca gcggtagatg cagtacgctg
     2821 tcgagcagag ctttgcccgg aaacgtgcaa cgcaccagta accaggcaaa aaagatccca
     2881 aacggcaggc taaacagcac agccagggaa gaaactttca ggcttaataa aactgcctgc
     2941 cattctggat cggtcagtat cattacttgg ttgtaaatcc gtaacgttta aagatttccg
     3001 ctgcctgcgg tcccttcaga taatcataaa atgctttcac tgtcgcattg ttatgccctt
     3061 ccacaacagc aaccggatat tccacttttt tatgtgaatc ttccgggaag gtggcaacca
     3121 cttttacccc tttgctggca actgcgtcag aaccgtagac aatgcccaga ggcgcttcgt
     3181 tacgttcgac cagcgccagc gccccacgaa catcttccgc tggggccagt ttcggagaga
     3241 gcgtatccca tgcgcccagt ttttgcagtg cttcttttgc ataaatgcca gcgggaacat
     3301 gttccggatc gccaaccgcc aggcgaccgc cattcagcag cgaagtccag ttagttttgc
     3361 tgtcgatggt gaaatctttc tgctcgctgg ctttcggtgc tacaacgacc aggctattgc
     3421 cgagcagtgt ctgacgcgta gcagtatcga tcgctttttt atcaaccgca taatccatcc
     3481 atttctgatc ggcagaaata aacagatccg caggggcacc cgcttcaatc tgacgggcga
     3541 gagtagatga cgaagcgaaa gaagaaacca catccacgcc tttctctttt ttatactgcg
     3601 tagcaatgtc ctgcattgcg ttagtcagtg atgcggcggc gaacaccgtg attttccctt
     3661 catctgccag tgcattgcca gcaactgcga aagagagtgc cgccccggca aacaagttca
     3721 accatttacg agccattggt aaccccttaa tgtaacgtta tgtaggcgac aatataacga
     3781 ctaactcgga attttcccag cagttattgc taacctttgt gaggtagata agaaaaaata
     3841 tcggcaggaa aagcaggaag ttgagaaaaa gaaaatgccc gactaagcgg gcattcaggg
     3901 aatcaatgat tttgtccggg ctggtctttt ttaccaacac cagaaaagat gttgaatact
     3961 tcaccaagac cgtaaatcag acccaggatg atggccatca cgacaggtac catgattacg
     4021 gcgaatacca gactttttaa taactctaac atggtcaact ccagatatag tcatgagact
     4081 attctaaccg ctaagcacag aaaagcactc cccttttgtg cggtcagctt tgcgtggcgt
     4141 tcgttttccg tcacaataag acttttgcca ggacattgtt atgcaggccg aaatccttct
     4201 cacccttaag ctccaacaaa aattattcgc cgacccgcgc cgcatttcgc tactaaaaca
     4261 cattgcgctt tccggttcca ttagccaggg agcgaaagat gccggtatca gctataaaag
     4321 cgcctgggat gccattaacg agatgaatca gttaagtgag catattctgg tcgagcgcgc
     4381 aacaggcggt aaaggtggcg gaggcgcagt actgacccgc tatggtcagc gactgattca
     4441 gctctatgac ttactggcgc aaatccagca aaaagccttt gatgtgttaa gtgacgatga
     4501 cgccctgccg ctgaacagcc tgctggccgc gatctcacgt ttttcactgc aaaccagcgc
     4561 ccgtaaccag tggttcggta ccatcaccgc ccgcgatcat gatgacgttc aacagcatgt
     4621 tgatgtctta ctggctgacg gaaaaacacg cctgaaagtc gcaattaccg cacaaagcgg
     4681 cgcacgtctg gggctggatg aaggcaaaga agtgttgata ttgctaaaag cgccgtgggt
     4741 aggcattact caggacgaag cggtcgcaca aaacgccgac aaccaattac cgggtattat
     4801 tagtcatatt gagcgcggcg cagagcagtg cgaagtatta atggcgctac ccgacgggca
     4861 aacactgtgc gccacagtgc cggtaaatga agcgacttct cttcagcaag ggcagaatgt
     4921 cacggcctac tttaatgccg acagcgtgat tatcgccacg ctgtgctaag cgtgttgaca
     4981 atttgttatg aaacacgtat ccctgtcagt aatcgctgca caaaatggga tataaaatgt
     5041 catcgttgca aattttgcaa ggcacgtttc gtcttagcga cacaaaaacg ctgcaattgc
     5101 ctcagctaac gttaaacgcg ggtgatagtt gggcgtttgt cggttcgaat ggaagtggaa
     5161 agtcggcgct ggcccgcgcg ctggcggggg aacttccgct tttgaaaggt gaacggcaaa
     5221 gccagttttc ccacatcact cgtctctcct tcgagcaatt gcaaaagctc gtcagcgacg
     5281 aatggcaacg gaataacacc gatatgctcg gccctagcga agatgacacc ggacgcacta
     5341 cggctgagat tattcaggat gaagtaaagg atgcaccgcg ttgcatgcaa ctggcgcagc
     5401 agttcggtat taccgccctc ctcgaccgac gctttaaata cctttccact ggcgagacgc
     5461 gaaaaaccct gctgtgtcag acgctgatgt cggagcctga cttgttgatt cttgatgagc
     5521 cgttcgatgg cctggatgtt gcctcacgtc agcagctggc tgagcgactc gcctcgttac
     5581 atcagtccgg tattactttg gtactggtgc tcaatcgctt cgatgagatc ccggaatttg
     5641 tccagtttgc tggcgtgctg gcggattgta cgttagcgga aactggcgct aaagaggaac
     5701 tgctccagca agcactcgtc gcgcaacttg cgcatagtga acagcttgaa ggtgtgcaac
     5761 tgccggagcc ggatgaacct tcagcacgtc acgccttacc cgccaacgaa tcgcgcattg
     5821 tgctgaacaa tggcgtggtt tcttataatg atcgccccat tcttaataac cttagctggc
     5881 aggtgaatcc aggcgaacac tggcaaattg tcgggccaaa tggcgcggga aaatcgacgt
     5941 tattaagcct gattactggc gatcatccgc aaggttacag caacgatttg acgcttttcg
     6001 gccgacgtcg cggcagcagc gaaaccatct gggacatcaa aaagcatatc ggttacgtca
     6061 gcagtagttt gcatctggat taccgggtca gcactaccgt gcgtaatgtg attctttctg
     6121 gctattttga ttcgattggc atttatcagg ccgtttcgga tcgccagcaa aaactggtgc
     6181 agcagtggct ggatattctc ggcattgata aacacacggc tgacgctccg ttccatagtc
     6241 tttcctgggg acagcagcgt ctggcgctga tcgtccgcgc actggtgaaa catccgacgt
     6301 tgcttattct cgatgaacca ctacaggggc ttgatccgct caatcgccaa cttatccgcc
     6361 gttttgttga tgtgctgatt agcgaaggtg aaacgcaatt gttgtttgtt tcgcaccacg
     6421 ctgaagatgc gcctgcctgt attacccatc gccttgagtt cgtgccggac ggtggactct
     6481 atcgctatgt gctgacaaaa atatattgag tcggtagtgc tgaccttgcc ggaggcggcc
     6541 taagcgccct ctccggccaa cggttcgacg catgcaggca tgaaaccgct tcttttttca
     6601 gataaaaagc gcaatcattc ataaaccctc tgttttataa tcacttaatc gcgcataaaa
     6661 aacggctaaa ttcttgtgta aacgattcca ctaatttatt ccatgtcaca cttttcgcat
     6721 ctttgttatg ctatggttat ttcataccat aagcctaatg gagcgaatta tgagagttct
     6781 ggttaccggt ggtagcggtt acattggaag tcatacctgc gtgcaattac tgcaaaacgg
     6841 tcatgatgtc atcattcttg ataacctctg taacagtaag cgcagcgtac tgcctgttat
     6901 cgagcgttta ggcggcaaac atccaacgtt tgttgaaggc gatattcgta acgaagcgtt
     6961 gatgaccgag atcctgcacg atcacgctat cgacaccgtg atccacttcg ccgggctgaa
     7021 agccgtcgcc gaatcggtac aaaaaccgct ggaatattac gacaacaatg tcaacggcac
     7081 tctgcgcctg attagcgcca tgcgcgccgc taacgtcaaa aactttattt ttagctcctc
     7141 cgccaccgtt tatggcgatc agccccaaat tccatacgtt gaaagcttcc cgaccggcac
     7201 accgcaaagc ccttatggca aaagcaaatt gatggtggaa cagatcctca ccgacctgca
     7261 aaaagcccag ccggactgga gcattgccct gctgcgctac ttcaacccgg ttggcgcgca
     7321 tccgtcgggc gatatgggcg aagatccgca aggcattccg aataacctga tgccatacat
     7381 cgcccaggtt gctgtaggcc gtcgcgactc gctggcaatt tttggtaacg attatccgac
     7441 cgaagatggt actggcgtac gcgattacat ccacgtaatg gatctggcgg acggtcacgt
     7501 cgtggcgatg gaaaaactgg cgaacaagcc aggcgtacac atctacaacc tcggtgctgg
     7561 cgtaggcagc agcgtgctgg acgtggttaa tgccttcagc aaagcctgcg gcaaaccggt
     7621 taactatcat tttgcaccgc gtcgcgaagg cgaccttccg gcctactggg cggacgccag
     7681 caaagccgac cgtgaactga actggcgcgt aacgcgcaca ctcgatgaaa tggcgcagga
     7741 cacctggcac tggcagtcac gccatccaca gggatatccc gattaaggaa cgaccatgac
     7801 gcaatttaat cccgttgatc atccacatcg ccgctacaac ccgctcaccg ggcaatggat
     7861 tctggtttca ccgcaccgcg ctaagcgccc ctggcagggg gcgcaggaaa cgccagccaa
     7921 acaggtgtta cctgcgcacg atccagattg cttcctctgc gcaggtaatg tgcgggtgac
     7981 aggcgataaa aaccccgatt acaccgggac ttacgttttc actaatgact ttgcggcctt
     8041 gatgtctgac acgccagatg cgccagaaag caacgatcct ttgatgcgtt gccagagcgc
     8101 gcgcggtacc agccgggtga tctgcttttc accggatcac agtaaaacgt tgccagaact
     8161 gagcgttgcg gcattgacgg aaatcgtcaa aacctggcag gagcaaaccg cagagctggg
     8221 aaaaacgtac ccgtgggtgc aggtctttga aaacaaaggt gcggcgatgg gctgctctaa
     8281 cccgcatccg cacggacaga tttgggcaaa tagcttcctg cctaacgaag ctgagcgcga
     8341 agaccgcctg caaaaagaat attttgccga acagaaatca ccaatgctgg tggattatgt
     8401 tcagcgcgag ctggcagacg gtagccgtac cgttgtcgaa accgaacact ggttagccgt
     8461 tgtgccttac tgggctgcct ggccgttcga aacgctactg ctgcccaaag cccacgtttt
     8521 gcggatcacc gatttgaccg acgcccagcg cagcgatttg gcgctggcgt tgaaaaagct
     8581 gaccagtcgt tatgacaacc tcttccagtg ctccttcccc tactctatgg gctggcacgg
     8641 cgcaccattt aatggcgaag agaatcaaca ctggcagctg cacgcgcact tttatccgcc
     8701 tctgctgcgc tccgccaccg tacgtaaatt tatggttggt tatgaaatgc tggcagagac
     8761 ccagcgagac ctgaccgcag aacaggcagc agagcgtttg cgcgcagtca gcgatatcca
     8821 ttttcgcgaa tccggagtgt aagaaatgag tctgaaagaa aaaacacaat ctctgtttgc
     8881 caacgcattt ggctaccctg ccactcacac cattcaggcg cctggccgcg tgaatttgat
     8941 tggtgaacac accgactaca acgacggttt cgttctgccc tgcgcgattg attatcaaac
     9001 cgtgatcagc tgtgcaccac gcgatgaccg taaagttcgc gtgatggcag ccgattatga
     9061 aaatcagctc gacgagtttt ccctcaatgc gcccattgtc gcgcatgaaa actatcaatg
     9121 ggcgaactac gttcgtggcg tggtgaaaca tctgcaactg cgtaacaaca gcttcggcgg
     9181 tgtggacatg gtgatcagcg gcaatgtgcc gcagggtgcc gggttaagtt cttccgcttc
     9241 actggaagtc gcggtcggaa ccgtattgca gcagctttat catctgccgc tggacggcgc
     9301 acaaatcgcg cttaacggtc aggaagcaga aaaccagttt gttggctgta actgcgggat
     9361 catggatcag ctaatttccg cactcggcaa gaaagatcat gccttgctga ttgactgccg
     9421 ctcactgggg actaaagcag tttccatgcc gaaaggtgtg gctgtcgtca tcatcaacag
     9481 taacttcaaa cgtaccctgg ttggcagcga atacaacacc cgtcgtgaac agtgcgaaac
     9541 cggtgcgcgt ttcttccagc agccagctct gcgcgatgtc accattgaag agttcaacgc
     9601 tgttgcacat gagctggacc caatcgtggc gaaacgcgtg cgtcatatcc tgactgaaaa
     9661 cgcccgcacc gttgaagctg ccagcgcgct ggagcaaggc gacctgaaac gtatgggcga
     9721 gttgatggcg gagtctcatg cctctatgcg cgatgatttc gaaatcaccg tgccgcaaat
     9781 tgacactctg gtagaaatcg tcaaagctgt gattggcaac aaaggtggcg tacgcatgac
     9841 cggcggcgga tttggcggct gtatcgtcgc gctgatcccg gaagagctgg tgcctgccgt
     9901 acagcaagct gtcgctgaac aatatgaagc aaaaacaggt attaaagaga ctttttacgt
     9961 ttgtaaacca tcacaaggag caggacagtg ctgaacgaaa ctcccgcact ggcacccgat
    10021 ggtcagccgt accgactgtt aactttgcgt aacaacgcag ggatggtagt cacgctgatg
    10081 gactggggtg cgactttact ttccgcccgt attccgcttt ccgatggcag cgtccgcgag
    10141 gcgctgctcg gctgtgccag tccggaatgc tatcaggatc aggctgcgtt tctgggggcc
    10201 tctattggtc gttatgccaa ccgcatcgcc aatagtcgtt atacctttga cggtgaaacc
    10261 gtgacgcttt cgccaagcca gggcgttaac cagctgcacg gcggcccgga agggttcgac
    10321 aaacgtcgct ggcagattgt gaaccagaac gatcgtcagg tgctgtttgc cctgagttca
    10381 gatgatggcg atcagggctt cccgggtaat ctcggcgcga cggtgcaata tcgtctgacc
    10441 gacgataacc gtatctccat tacttatcgc gccacagttg ataaaccttg cccggtgaat
    10501 atgactaatc acgtctattt caatcttgac ggcgagcagt ctgacgtgcg caatcacaag
    10561 ttgcagattc tggcggacga atatcttccg gttgatgaag gcggcattcc gcacgacggc
    10621 ttgaaatctg tcgccggaac gtcttttgat ttccgcagcg ccaaaatcat cgccagtgag
    10681 tttcttgccg acgacgatca gcgcaaagtg aaaggttacg atcacgcatt cttgttacag
    10741 gccaaaggcg atggcaagaa agtggcggcg catgtctggt cagcagatga aaaattgcag
    10801 ctgaaggtct acaccaccgc tccggctctt caattctact ccggcaactt cctcggcggc
    10861 acaccgtcgc ggggaaccga accttacgcc gactggcaag ggctggcgtt ggaaagcgag
    10921 tttctgccgg acagcccgaa ccaccctgaa tggccgcaac cggactgctt cctgagtcct
    10981 ggcgaagagt attccagcct gacggaatat cagtttattg ctgagtaatg ttaagccctc
    11041 gtcatgaggg ctttatcttc atattgttca aatcaccagc aaacaccgac atatttgcaa
    11101 ctcaatattc acaacaacct tacactgcgc cactattttc gctatggtta tgcgtaagca
    11161 ttgctgttgc ttcgtcacgg caacataatg agaattatta tcattaaaag atgatttgag
    11221 gagtaagtat atggctgtaa ctaagctggt tctggttcgt catggcgaaa gtcagtggaa
    11281 caaagaaaac cgtttcaccg gttggtacga cgtggatctg tctgagaaag gcgtaagcga
    11341 agcaaaagca gcaggtaagc tgctgaaaga ggaaggttac agctttgact ttgcttacac
    11401 ttctgtgctg aaacgcgcta tccataccct gtggaatgtg ctggacgaac tggatcaggc
    11461 atggctgccc gttgagaaat cctggaaact gaacgaacgt cactacggtg cgttgcaggg
    11521 tctgaacaaa gcggaaactg ctgaaaagta tggcgacgag caggtgaaac agtggcgtcg
    11581 tggttttgca gtgactccgc cggaactgac taaagatgat gagcgttatc cgggtcacga
    11641 tccgcgttac gcgaaactga gcgagaaaga attgccgctg acggaaagcc tggcgctgac
    11701 cattgaccgc gtgatccctt actggaatga aaccattctg ccgcgtatga agagcggtga
    11761 gcgcgtaatc atcgccgcac acggtaactc cctgcgtgcg ctggtgaaat atcttgataa
    11821 catgagcgaa gaagagattc ttgagctgaa tatcccgact ggcgtgccgc tggtgtatga
    11881 gttcgacgag aatttcaaac cgctgaaacg ctattatctg ggtaatgctg acgagatcgc
    11941 ggcgaaagca gcagccgttg cgaaccaggg taaagcgaag taaacgtcat tcgtttaaaa
    12001 aatgagaaag ccgactgcaa gtgagtcggc ttttttgttt gtcgttgcta acaatggagc
    12061 acattgcctg atgcgacgct gcgcgtctta tcaggcctgt ggtgattcat cggatacgcc
    12121 actctgacgg cgcatccgac aattaaacct tacccgcgac gcgcttttac tgcattcgcc
    12181 agttgacgta acagagcatc ggtatcttcc cagccaatgc aggcatcggt gatgctctta
    12241 ccgtaggcca gcggctcccc gctatcgagg ctctgattgc cttccaccag atggctttcc
    12301 accatcacgc caataatggc cttttcgcca ccggcaatct gctggcaaac gtcagcacaa
    12361 acatccatct gctttttgaa ttgtttggac gaattagcat ggctgaaatc gatcatcacc
    12421 tgagctggca gacctgcttt gttcagccct tctttcactt cagcaacgtg cttcgcgctg
    12481 tagttaggct ctttaccgcc gcgcagaatg atatggcaat cgccgttacc actggtattc
    12541 acaatcgccg aatgccccca tttagttacg gacaggaagc agtgcggcgc accggcggca
    12601 ttaatggcat cgatagccac tttaatcgta ccgtctgtgc catttttaaa acctaccgga
    12661 caagaaagac cagacgccag ttcgcggtgc acctgcgatt ccgtggtacg tgcaccaatt
    12721 gcgccccagc tcatcaggtc agcgagatac tgaggagtga tcatatcgag aaactcaccc
    12781 gccgctggca gaccgctgtc gttaatatca agcagcaatt tacgggctat acgcagaccg
    12841 tcgttgatct ggaagctgtt atccatatgc ggatcgttaa tcaacccttt ccagcccacc
    12901 gtggtacgcg gcttttcaaa atagacgcgc attacgattt ccagctcatc tttcagctct
    12961 tcacgcagcg ccagcaagcg agtggcatac tctttagccg cgacaggatc atgaattgag
    13021 cacgggccaa tcaccaccaa caggcgatca tcattacctt tcaggatctt atggatcgct
    13081 tttcgggcat gggcaaccgt attcgcggca ttttcagttg cggggaattt ttccagcaat
    13141 gcgacaggag gaagtaactc tttgatttct ttgatgcgta aatcgtcgtt ctgataattc
    13201 atgtctgttc cagtgttgcc atacttatct tagtgaatgc aatacttcca atctatatct
    13261 tccgtcagaa tgtgtaaacg gggttttaca ctatgaacgg attaatcctg gaattagcaa
    13321 gaaaaacgcc agattgtcgc gaaaaacgag atctatccta caatttctaa ctgtaactcc
    13381 tttgtttgtt aattatttca agattctctg ctgcgtttca taaactggct gaaatcttaa
    13441 accaatgcct tatattcacc tgcaaatgca ctgttggaag aggttatccg acataacgac
    13501 cataacagga gcatcctatg aaaatgacaa aactggccac attatttctg actgccactc
    13561 taagccttgc cagcggtgcc gcactggctg ccgatagcgg agcgcaaact aataacggcc
    13621 aggcaaacgc cgcagctgat gcgggccagg tagcccctga cgcccgtgaa aatgtcgcgc
    13681 caaataacgt cgacaataac ggggtaaata ccggttctgg cggcacaatg ctgcattcgg
    13741 atggttcttc aatgaacaat gacggaatga ccaaagatga agagcacaag aacaccatgt
    13801 gcaaagatgg tcgatgtccg gatatcaata aaaaggtaca aaccggtgat ggcatcaata
    13861 atgatgtcga taccaaaacc gacggtacca cgcagtaacc ctgcaaaata gccaataatg
    13921 ggagagcgga gtctctccct tttaattttt cacttctaaa agcagtatga tgtcataaag
    13981 ttaagtgaga ataattaagg gatgactcgt catggcgcac tcacacacgt cctcacacct
    14041 gccagaagat aataatgctc gtcgcttgtt gtatgctttc ggcgtgactg ccgggtttat
    14101 gctggtagaa gtcgttggtg gttttctttc tggttctctg gcattgctgg ccgatgcggg
    14161 tcatatgttg accgatactg ccgccctgct tttcgcccta ctcgccgtgc aattttcacg
    14221 tcgtcctccc accattcgcc acactttcgg ttggctaaga ctgaccacgc tcgcggcgtt
    14281 tgtgaatgcc atcgccctcg tggtaattac cattttgatt gtctgggagg cgatagaacg
    14341 cttccgcacg ccgcgtccgg tcgagggagg catgatgatg gcaattgccg tggctgggtt
    14401 gctggcaaat atactttctt tctggttact tcatcacggc agcgaagaga aaaacctcaa
    14461 cgtacgagca gcggcactgc atgtgctcgg ggacctgctg ggttcggttg gggctattat
    14521 tgccgcgttg attattatct ggactggctg gacacctgct gaccccattc tctcgatact
    14581 ggtgtcgctt ctggttctgc gtagcgcgtg gcgactgttg aaagatagcg tgaatgaatt
    14641 acttgaaggt gcaccggtat cgctggatat cgctgaactg aagcgtcgta tgtgccggga
    14701 aatcccggaa gtccgcaatg tgcaccatgt acatgtatgg atggtaggcg agaagccggt
    14761 gatgacgttg catgtgcagg tgatcccgcc acacgatcac gatgccttgt tggatcagat
    14821 ccaacactac ctgatggatc actatcagat tgagcacgcc accattcaga tggaatatca
    14881 accttgtcat gggccagact gccacctgaa tgaagggggg tctggccatt cacatcatca
    14941 ccattaatgg gacagcgcgc gtgagcctct ttcacgtgcg ctgttgatcc acatccggct
    15001 gccgttgagc gcaataaagg tcaggatgat gtactccaga gacatggcgt aaacgccctg
    15061 aagtgcaaaa ataacgacgc taatcacgtt aataatcacc cacaacagcc agttttccac
    15121 atacttacgc gtcatcagaa tcattgccac gatagataac accatcatgc atgaatccca
    15181 gaacgggaaa gcgtccggtt gcagttcagg catcacaacc tgtaatccta atgcttgcat
    15241 gatcatgact gccacgcggg tcaaaaatgc aaacaccgga ttgataaata ccgtcatcag
    15301 accaatcgaa acaacgcaaa ccgccaacca gctgagtgcc ttcggcaatg gcaaccagcg
    15361 aattttcaac tccgcctcgt tctgactggt ttgtcgcgac cacgcatacc agccgtaaat
    15421 attcgcggca aagaaaaaca cctgtaatag caggctggca tacagctgaa tctgaaagaa
    15481 aataatgcca aacaaggtaa cgttgatcag gccaaagaag tagttgctga tcttctccag
    15541 actggcaagg ccaatacaca gcaacccggc gatcgtgcct accgcttcga tccatgagag
    15601 atcataaccg cccgccccta ttggtatatg tactaggata ttctgcacac taaaaaaatc
    15661 catctttccc ccaaagcgta acgttacgcc ttattattcg ttatccacgt agtgtagccg
    15721 caaaatccag catacgattg agcggcacca gcgccctctc tcgcagcctc tcatcgacat
    15781 gaacctcgtg attacttcct tcctgttcta atgcctctgc gatagcctga aggccattca
    15841 tggccatcca cggacaatgc gcgcagctgc ggcaggttgc gccctcaccc gcggttggtg
    15901 cttccagtaa ctctttatct ggcaccgcct gctgcatttt gtagaaaata ccccgatcgg
    15961 ttgccacaat aagcctctga tgaggcaatg cttttgcagc agcgatcagt tgactggtgg
    16021 aaccgaccgc atccgccata tcgacaatag cttgtggtga ttctggatgc accagtatgg
    16081 cagcatccgg gtattcttct tgcaagcggg ttaacgcctg agtcttaaat tcatcatgca
    16141 caatgcaggc accctgccag cataaaatgt ctccacccgt ctgtttttgc acgtaacgcc
    16201 ccagatgttt gtcgggtgcc cagatgattt tttcacccaa actatcaaga tgatcaataa
    16261 gttcgacggc aatgcttgaa gttaccaccc aatctgcgcg cgcttttacc gcagcagaag
    16321 tgttggcgta gacgacgaca gtacgatcgg gatgggcatc gcaaaatgcg ttaaattctt
    16381 caacagggca gccgagatcc agtgaacact cagcctgaag tgtcggcatc agaattgttt
    16441 tttccggact gagaattttg gcggtttctc ccataaatct caccccagcg actaacaaag
    16501 tagaagcggg atgctttgca acgaagcgcg ccatttccag agaatcagaa atacagccac
    16561 cagtttcttc tgccagttgt tgaatttcgg gatcggtata gtagtgggca accatcaccg
    16621 cattacgttc ttttagcaga cgttttatct tctcgcggta atacgctttt tcatcaatgc
    16681 ttaacggcgt cggcttcggg gggaaaggat aaatcgccgt gtctggatca aacattacgc
    16741 tcatcttacc atctcgtttt acaggcttaa cgttaaaacc gacattagcg taacattcgc
    16801 tgttatcggt gtaatttgtt ttatatgcta aacaagatag tcgaatggtg gggaaaagtc
    16861 actcgaatta cgcctaattc caccgacgta gagttgcttg cacagtaagc tctgacgatg
    16921 tcatcaggtt tacaaagtga aattgaggat gcctgaatag caaaaaagcg cctttagggc
    16981 gcttttttac attggtgggt cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca
    17041 actgctctac caactgagct aacgacccat acgggtgtta cctgaaagat ttctactcgg
    17101 ggccaatctt taagattggt gggtcgtgca ggatgactcg cttcgctcgc cctacgggcc
    17161 gttgctggcg caacgttatc cttcacgctt aacatctgag tttgatgtta aaattggtgg
    17221 gtcgtgca
//
LOCUS       DBKEKI010000079        16909 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.079, whole genome shotgun sequence.
ACCESSION   DBKEKI010000079 DBKEKI010000000
VERSION     DBKEKI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.079"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..134
                     /locus_tag="ACUQPX_002620"
     CDS             <1..134
                     /locus_tag="ACUQPX_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057297.1"
                     /translation="GTKVYTSTQVGKECQFTTGLAVVITTTYNETRIQPNTKCPEKS"
     gene            150..667
                     /locus_tag="ACUQPX_002621"
                     /pseudo
     CDS             150..667
                     /locus_tag="ACUQPX_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
     gene            complement(762..2339)
                     /gene="guaA"
                     /locus_tag="ACUQPX_002622"
     CDS             complement(762..2339)
                     /gene="guaA"
                     /locus_tag="ACUQPX_002622"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HHV9057298.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2408..3874)
                     /gene="guaB"
                     /locus_tag="ACUQPX_002623"
     CDS             complement(2408..3874)
                     /gene="guaB"
                     /locus_tag="ACUQPX_002623"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HHV9057299.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4036..5406
                     /gene="xseA"
                     /locus_tag="ACUQPX_002624"
     CDS             4036..5406
                     /gene="xseA"
                     /locus_tag="ACUQPX_002624"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HHV9057300.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQFWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5403..5618)
                     /locus_tag="ACUQPX_002625"
     CDS             complement(5403..5618)
                     /locus_tag="ACUQPX_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HHV9057301.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(5688..7160)
                     /gene="der"
                     /locus_tag="ACUQPX_002626"
     CDS             complement(5688..7160)
                     /gene="der"
                     /locus_tag="ACUQPX_002626"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HHV9057302.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7278..8456)
                     /gene="bamB"
                     /locus_tag="ACUQPX_002627"
     CDS             complement(7278..8456)
                     /gene="bamB"
                     /locus_tag="ACUQPX_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HHV9057303.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8467..9087)
                     /gene="yfgM"
                     /locus_tag="ACUQPX_002628"
     CDS             complement(8467..9087)
                     /gene="yfgM"
                     /locus_tag="ACUQPX_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HHV9057304.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9105..10379)
                     /gene="hisS"
                     /locus_tag="ACUQPX_002629"
     CDS             complement(9105..10379)
                     /gene="hisS"
                     /locus_tag="ACUQPX_002629"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HHV9057305.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKQLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10490..11608)
                     /gene="ispG"
                     /locus_tag="ACUQPX_002630"
                     /gene_synonym="gcpE"
     CDS             complement(10490..11608)
                     /gene="ispG"
                     /locus_tag="ACUQPX_002630"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HHV9057306.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(11635..12648)
                     /gene="rodZ"
                     /locus_tag="ACUQPX_002631"
     CDS             complement(11635..12648)
                     /gene="rodZ"
                     /locus_tag="ACUQPX_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HHV9057307.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(12933..14087)
                     /locus_tag="ACUQPX_002632"
     CDS             complement(12933..14087)
                     /locus_tag="ACUQPX_002632"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HHV9057308.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14237..14668)
                     /gene="ndk"
                     /locus_tag="ACUQPX_002633"
     CDS             complement(14237..14668)
                     /gene="ndk"
                     /locus_tag="ACUQPX_002633"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HHV9057309.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(14817..16909)
                     /gene="pbpC"
                     /locus_tag="ACUQPX_002634"
                     /pseudo
     CDS             complement(14817..16909)
                     /gene="pbpC"
                     /locus_tag="ACUQPX_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; internal stop;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
BASE COUNT         4068 a         4725 c         4212 g         3904 t
ORIGIN      
        1 aaggcaccaa agtgtatacc tccacccagg tgggtaaaga gtgccagttt acgacaggtt
       61 tagccgttgt tattaccaca acgtataacg aaacgcgtat tcagccaaat accaaatgtc
      121 ctgaaaagag ctaataatca ggaggagtca tgaagaaagt ttttctttgc gccatcttag
      181 cctccttaag ctatccggct atagcctcat cattgcagga tcaactctct gctgtcgcag
      241 aagcggaaca gcaaggtaaa aatgaagagc aaaggcagca tgacgaatgg gtcgcggagc
      301 gcaacaggga aatccagcaa gagaagcaac gtcgcgcaaa tgcccaggcc gccgcgaaca
      361 aaagagcggc aacggcagcg gcaaataaga aagctcgtca ggataaactg gacgccgaag
      421 ccactgcgga caaaaaacgc gatcaaagtt atgaagatga gctacgcagc ttagagattc
      481 agaaacaaaa actggcgctg gcgaaagaag aagcccgcgt taagcgcgaa agaagcacaa
      541 agctgcgcaa accgatgtgg tgcaatctga agctgacgca aacagaaata tgactgaagg
      601 cggtcgcgat ctgatgaaaa gcgtgggcaa agcagaagag aacaaatcgg acagctggtt
      661 taattaatcg atgttagtaa cttcaagcct atgattcttg aagataaaaa accctctgta
      721 gtaacggagg gttttgttca ttcatagtgc agggatcaaa atcattccca ctcaatggta
      781 gctggcggct tgccgctgat gtcatacacc acgcgggaaa taccgttcac ttcattgata
      841 atgcggttgg aaacgcgacc gaggaaatcg tacggcagat gcgcccagtg tgcggtcata
      901 aagtcgatgg tttcgacagc acgcagagag acaacccagt catacttacg accatcgccc
      961 attacgccaa cggaacgtac tggcaggaag acggtgaacg cctggctgac tttgtcgtac
     1021 aggtccgctt tacgcagttc ttcaatgaag atggcgtcag cacggcgcag caggtcacag
     1081 tactctttct tcacttcacc cagaacacga acgccaaggc ctggtcccgg gaacggatga
     1141 cggtacagca tgtcgtacgg caggcccagc tccagaccaa tcttacgcac ttcgtctttg
     1201 aacagctctt tcagcggttc aaccaggccc atcttcatct ctttcggcag gccgcccacg
     1261 ttgtggtgag atttgatgac gtgtgcttta ccggttgcag aagccgcaga ttcgataacg
     1321 tcagggtaga tggtgccctg cgccagccac ttcacgtctt ccagtttcag cgcttcttca
     1381 tcgaatactt caacgaaaac gcgcccgatg attttacgtt ttgcttccgg atcgttttcg
     1441 ccagccagcg ctgacaggaa gcgatcttct gccggtacgt gaacaatgtt aagaccaaag
     1501 tgatcgccaa acatatccag aacctgctct gcttcgttga gacgcagcag gccgttgtcg
     1561 acgaatacgc aagtcaggtt tttaccgata gcacggtgca gcagcattgc ggttacggag
     1621 gaatccacac caccagagag gccgaggatg actttatcgt cgcctacctg ctcgcggatg
     1681 cgagctacag catcgtcgat aattttcgct ggcgtccaca gggcttcaca ctggcagata
     1741 tcacgcacaa aacgctccag catgcgcata ccctggcggg tgtgagtcac ttccgggtgg
     1801 aactgtacgc catagaagcg tttttcttcg ttagccataa tggcaaacgg gcagctttcg
     1861 gtgctggcta cggtgacgaa gtcggacggg atagcggtaa ctttatcgcc gtggctcatc
     1921 cagacatcga gcagcggttt accgtctgcg gtcagcgcat cttcgatacc gcgaaccagt
     1981 gcgctgtcgt ttacgacttc aacctgcgcg tagccaaatt cacgttcgtt agaggcttca
     2041 acgtgaccgc ccaactgcat tgccatggtc tgcatgccat agcaaacgcc gaatatcggt
     2101 acgcctgctt caaagacata ctgcggcgca cgcggactgt tttcttcagt agtactttcc
     2161 gggccgccgg aaagaataat gccgcttgga ttgaagtcac gaatttgtgc ttctgtcaca
     2221 tcccacgccc acagttcgca gtaaacaccc agctcacgca cgcggcgcgc aaccagttga
     2281 gtgtactgag aaccgaagtc cagaatgagg atgcgatgct tatgaatgtt ttccgtcatt
     2341 gacgcttatt ccgaggcaag tgaaacagat aatataaatc gcccgacatg aagtcgggcg
     2401 aagagaatca ggagcccaga cggtagttcg gggactcttt agtaatggtc acgtcgtgaa
     2461 cgtggctttc ctgaataccc gcaccgctga tacgtacaaa ctccgcttta gtacgcagtt
     2521 cgtcgatagt accacagccg gtcagaccca tacaggagcg caggccgccc atctgctggt
     2581 gaatgatctc tttcaggcga cctttataag ctacgcgacc ttcgatacct tccggcacca
     2641 gtttgtcggc agcgttatcg ctctggaaat aacggtcaga ggaacctttg gacatcgcgc
     2701 ccagggaacc cataccacgg taagatttat aagaacggcc ctggtagagt tcgatttcac
     2761 ccggagattc ttcagtaccc gccagcatgg aacctaccat caccgcgctt gcgccagcgg
     2821 cgatagcttt ggcgatgtcg ccggagaagc gaataccacc atctgcgata accggaatac
     2881 cggtgccttc cagggcttca actgcgtcag caacggcagt gatctgcgga acacctacgc
     2941 cagtaacgat acgagtcgta cagatagaac cagggccgat accgacttta accgcgctgc
     3001 aaccagcttc tgccaaagcg cgtgcacctg cagctgttgc cacgttgccg ccgatgattt
     3061 gcagatccgg atatttagca cgggtttcac ggatacgctg caaaacgcct tctgagtgac
     3121 cgtgggagga gtcgatcagc agaacgtcaa cgccagcggc aaccagcgcg tcaacacgct
     3181 cttcgttacc cgcacctgcg ccaactgcag caccaacacg cagacggcct tgctcgtctt
     3241 tacaggcgtt cggtttacgt tccgctttct ggaagtcttt cacggtgatc atgccgatca
     3301 ggtggaattc gtcatcaacc accagcgctt tttcaacgcg tttttcgtgc atttttgcca
     3361 gcaccacttc acgggcttca ccttcacgca cggtgaccag acgctctttc ggcgtcatgt
     3421 aaacgctaac cggctggttc aggtcggtaa caaaacgcac gtcacgaccg gtgataatac
     3481 ccaccagttc gttttcttcg gtaacgaccg gatagcccgc aaaaccgtta cgctcggtca
     3541 gttctttcac ttcgcgcagc gtcgtggttg gcagaacagt ctgcggatca gtcaccacac
     3601 cagattcgtg ttttttcaca cggcgaactt cttctgcctg gcgttcaatg gacatgtttt
     3661 tgtggataaa gccgataccg ccttcctgag ccagagcaat agccaggcgc gcttccgtta
     3721 cggtatccat tgctgcggaa agcataggga tattcagacg aatagttttc gtcagctggg
     3781 tgctgaggtc agcagtattc ggcagaacgg tagaatgagc aggaacgagg agaacgtcgt
     3841 caaacgtcag agcttcttta gcgatacgta gcatgggcaa tatctcgacc agagtggtta
     3901 ataaatattg ccgcggcatt atacagagcg taaccgattg catctacccc tttttgcaaa
     3961 aaatgcttgc tatccccgaa gggcgggtta ctatcgactg aataacctgc tgatttagaa
     4021 tttgatctcg ctcacatgtt accttctcaa tcccctgcaa tttttaccgt tagtcgcctg
     4081 aatcaaacgg ttcgtctgct gcttgagcat gagatgggac agttttggat cagcggcgaa
     4141 atctctaatt tcacacaacc ggcttccggt cactggtact ttacactcaa agacgacacc
     4201 gcccaggtac gctgcgcgat gttccgcaac agcaaccgcc gggtcacctt ccgtccgcag
     4261 catgggcaac aagttttagt tcgcgccaat attacgctct acgagccgcg cggcgactac
     4321 cagataatcg ttgagagtat gcagccggcc ggtgaagggc tgctgcaaca gaagtacgaa
     4381 cagctcaaag cgaagttgca ggctgaaggt ttgttcgatc agcaatacaa aaaaccactt
     4441 ccctcccctg cgcattgcgt tggtgtgatc acctcaaaaa ccggtgctgc gctacatgat
     4501 attttgcatg tgttaaaacg tcgcgatcct tctctaccgg tgatcatcta ccccacctcc
     4561 gttcagggcg atgacgcgcc ggggcaaatt gttcgcgcca ttgaactggc gaatcagcgc
     4621 aatgagtgcg acgtgttgat cgttgggcgc ggcggcggtt cgctggaaga tttatggagt
     4681 tttaacgacg aacgcgtagc gcgggcgatt tttgccagcc gcattccggt cgtcagcgcc
     4741 gtcgggcatg agacggatgt gaccattgcc gattttgttg ccgatctgcg tgcgccaacg
     4801 ccgtctgccg ccgctgaagt agtgagccgt aatcagcaag agttactgcg ccaggtgcaa
     4861 tcggcccgtc aacggctgga gatggcgatg gattattatc tcgccaaccg tacgcgtcgc
     4921 tttacgcaga ttcatcatcg cttgcagcag cagcatccgc agctccggct ggcacgccag
     4981 caaaccatgc ttgaacgcct gcaaaaacgg atgagctttg cgctggaaaa tcagcttaag
     5041 cgtgccgggc aacagcagca gcgattaaca cagcggctga atcagcaaaa tccacagccg
     5101 aagattcatc gcacgcaaac gcgcattcag caactggaat atcgtttagc agaaatcctg
     5161 cgcgcacagc ttagcgccac gcgtgaacgt ttcggtaatg cagtaacgca cctcgaagcc
     5221 gtaagcccac tgtcaacgct ggcgcgtgga tacagcgtta ctactgctac tgacggcaat
     5281 gtactgaaaa aagtgaagca agttaaagcg ggtgaaatgc taaccacacg tctggaagac
     5341 ggctggatag aaagtgaagt aaaaaacatc cagccggtaa aaaaatcgcg taaaaaggtt
     5401 cattaagcca gcacgaattc aacgcgtttt ttcgatatca agccatgccc gtgctgacag
     5461 aagtaatcta ctgcaccgca ggcttttaac acctgcaacg gttgatggca gtctggacaa
     5521 agagctttca tttcaataaa ttccccgcag gatgggcaac gcgcatggcc gttatcctga
     5581 tcaagcacat gctgacattt tggacaatgc agttccatat gaattcctcc ggtagccatt
     5641 ccctctacat tcatagaggg aatggcagat aaaatactta cggataatta tttatttttc
     5701 ttgatgtgct tcatcagacg cttacgttta cgcatctggg ttggcgtcag ggtgttacgc
     5761 ttgttcgcat acgggttttc cccttctttg aactgaatac gaatcggcga tcccattacg
     5821 tccagcgatt tgcggaagta gttcatcaag tagcgcttgt aggaatcagg caggtctttc
     5881 acctgattac cgtgaatcac cacaatcggc gggttataac caccagcgtg ggcatatttc
     5941 agcttcacac gacgaccgcg taccagcggc ggttggtgat cttcaacagc catcgtcatg
     6001 atgcgcgtca gcatagaggt ccccacacga cgggtggagc tgtcatacgc ttcacgtact
     6061 gattcaaaca agttaccaac accactgccg tgcaaggcag agataaagtg cacacgagca
     6121 aaatcgataa agcccagacg gaagtccagc gtttctttca cctgctcttt cacttcctgg
     6181 ctcaggccat cccacttatt caccacaatg acaagtgagc gcccactatt gagaataaag
     6241 cccagcagcg agagatcctg atcggaaata ccttcgcgcg catcaatcac taacatcacc
     6301 acgttggcgt cttcaatggc ctgcaacgtt ttgattacgg agaatttctc tacagcatcg
     6361 gtgattttgc cgcgtttacg tacgccagcg gtgtcaatga gcacatactc acgtccatcg
     6421 cgttccattg ggatgtagat gctgtcacgc gtcgtgccag gcatgtcgta aacaacaacg
     6481 cgctcttctc caagaatacg gttagtgagt gtagacttac ctacgttcgg acgacccaca
     6541 atcgccagtt tgatcggcag actttgcggg tcgaagtcgt cttcctcttc ttcttcgccg
     6601 ttctcttccg cttcaaattg cgcccagtat tcagcgtctt cgtcgacttc ctcttgcggt
     6661 gcgagatctt ccatccacgg cagcagcaca tgctccagca gacttaatac gccacgaccg
     6721 tgagacgcgg cgatcgggta gatttcacct aaaccaagcg agtagaaatc aaccactgcc
     6781 tgatcgggat ccagaccgtc agttttgttt gccaccagga aggtcggttt ttcacgggag
     6841 cgcagatgtt tggcaatcgc ttcatctgcc ggcatcaggc ccgcgcgcgc atccaccata
     6901 aacagtacga cgtccgcttc ttcaatcgcc agcagcgact gttccgccat gcgggtttct
     6961 acaccgtctt ctgtgccatc aatcccgccg gtatcaatac agataaactc acggccttca
     7021 atttccgcac gaccgtactt acggtcacga gtcagacccg ggaaatccgc aaccagcgca
     7081 tctcgggtgc gagttaaacg gttaaataac gtggattttc ctacgttagg gcgcccgaca
     7141 agcgcgacca caggtaccat gtttaaagcc tcatttttat aaatcatcag acaacgcacg
     7201 ctatattcgc gtcgttgtta aaaacaggaa aacggcccct gtccaggagc cgttttcaaa
     7261 gtgaacgaca gagacgatta acgtgtaata gagtacacgg ttccgtcttt tgcctggatc
     7321 agcagtttgc cgtcagcggc aaccggttca gtctggaaac cggaactatc aactttttgc
     7381 tgggcaacga aacgaccatc ttcgacgtta atccagtgca gataaccttc actgtcaccg
     7441 accaccaggt tgccattata cagcaccgga gaagtcagca ggcgatgcag cagatcgctt
     7501 tgtgcccaca gcgtaacgcc gccatcaatg gtcaacgcca tcacccggtc attttgatcg
     7561 accagataga tgcgattgcc atcgacgatg aaatcattca ccgaacccag ttcgcgtttc
     7621 cacataatct gaccactgcg cagatcaagc gccgtcaggt taccattata ggccagcgcg
     7681 aaaacaacgc cgttaacaac gacgggagtc gtgtcaacat cgctcagacg gtcaatttcg
     7741 gtagaaccgg tcgcctggga aatacgctgc tgccaaatca tctggccctg ttccatcagc
     7801 actgcgctga cgcgaccatt atcgccaccc acgacggccg caccaaaagc cgttgccggc
     7861 gcagactcgc cacgcaaaga gagcgaaggc atatcgaggt taactgtcca tttgacagcg
     7921 ccgtcagctt cgttcagcgc ttgtaactga ccgttactgg tgtggattaa caccagaccg
     7981 tcgctgacca ccgggcgcga aagtgcttca cccgcgactt tagtttgcca tgccacagta
     8041 ccatcgctgg tattcagcgc gtaaacctgc gccttttcgc tgccaatgta gacatgccca
     8101 ccagacacgg tcacaccgcc agaaagtaat gcaggctctt tagagaacca gccatctttc
     8161 tcggccaggc tgacagacca gatttctttg ccatcatccg cattcagcgc ttttaccaaa
     8221 ccagcgcggt ccgctgcata gacaacgttg tccgccagtg ccggatgaag attggaatag
     8281 aagttgccaa tgccgctacc aacggaagtg ctccacgccg tggtcggcgt aaactggttt
     8341 tcaacggttg gcaatgggga catctttacc acatcttctt cgctgttaaa cagcgaacag
     8401 ccgcttaaaa gggtaacgga aagcagtcct ggcagcagta atttacgcaa ttgcatcggg
     8461 tccctctcag atggacaaat tattaatttt catctgcatc atttcgctca gtgccggagt
     8521 aacatcgctt ttcacgcctg cttcccatgc actacgcgca ccttgcttat cacctttgct
     8581 cagcaatgct tcaccacgca ggtcggcaac aatggcagcc cacccttcac ctttgatggt
     8641 atcaagggtt ttcagcgcgg catcagcctg cttgagctgt acctgaacgc gagcaagacg
     8701 cagatttatc acggctttga gattttcatc gctcgtgtct gccagcccct gttgtaactg
     8761 ggcggcagct ttctctagtt catttttgtc aacaaattgc tgcgcaagtt ccaaagaagc
     8821 cagcgcacca taagtatttt tattttcagc agcaaatttt tccgccgccg ggatgctatc
     8881 cggtttgcct tcgctcactg cggtaaccgc attttgatag gcaagagaag cggagcgtgc
     8941 agaatcaacc tgatggctgt tccagtagcg ccagccaatc agtgcgccaa cgcccaaaat
     9001 caccccaaca gccagtgctt tgccattttc agcaaaaaag cgtttaaccg cttctacctg
     9061 gtcgttttcg ttctcgtaaa tttccacgct gtccttctcc ttccttaacc cagtaacgtg
     9121 cgcaaatgcg cggctacgct atcctgcgca actgccgttt gctcaccaga gcgcaaatcc
     9181 ttcactactg ctgtgccgtt agccacttca gactcaccca gcaccacagc aacgcgggca
     9241 ccccatttat cagcacgggc aaactgtttc ttaaagttgc cgccgccgtg gttggtcatc
     9301 aatttcacgc ccagtaattc atcacgcaga cgctcagcta atgccatagc cgcagattgt
     9361 gtatcagcac ctgaagccac caggtatata tcgacaacag gatcggcttt aaattccgga
     9421 ttaacggcct gtactaacaa tacaagacgt tcgaggccca tcgcaaaacc gacagccggt
     9481 gttgcacgac cgcccagttg ttccacaaga ccgtcataac gaccgcctgc acacacggtg
     9541 ccctgggagc cgagactgtt agtcacccac tcgaaaacgg tacggttata gtaatccaga
     9601 ccacgcacca gacgctggtt tacggtgtaa gcgatccccg cgctctccag ctgtttgcac
     9661 agaccggcaa aatgctcacg agattcctcg tccagataat cacctaatgc cggagcgtcg
     9721 ttgagaagcg cctgcacttc cggatttttt gaatccagca cgcgcagcgg gttagtgtac
     9781 atgcggcgtt tgcagtcttc gtccagcttt tctttatgct gctcaaggaa tgccaccagc
     9841 gcatcgcggt aattggcgcg tgcttccagc gaaccgatag aattcagctc aagagttacg
     9901 tgttcgaaga tacccagcgc acgccaccag cgggcagtga gcataatcag ttcagcgtcg
     9961 atatccggac cttgcagacc gaaaacttcg cagcccaact gatggaactg acgataacgc
    10021 cctttctgcg gacgctcgtg acggaacatc ggcccgatat accacagacg ctgttcctga
    10081 ttgtacagaa gaccatgctc gatgccggcg cgtacacagc ccgccgtccc ttcagggcgc
    10141 agagtcaggc tgtcgccatt gcgatcctca aaggtgtaca tctctttttc aaccacgtcg
    10201 gtgacttcac caatcgcacg tttgaatagc ggggtctgct ctacaatcgg caagcggatt
    10261 tcactgtaac cgtagctgcc gagcacgttt ttcagtgtgc cttcaatgcg ctgccagatg
    10321 gccgtttcgc caggcaggta atcgttcatg ccgcgaatgg cttgaatgtt ttttgccacg
    10381 tttattctct ttctgaatat aaaaatgaac cctcaacgct tccctcaatg tttcgggagc
    10441 catgcgggtt caatcataca cgggaagcga ggcgcttccc atcacgttat tatttttcaa
    10501 cctgctgaac gtcaattcga cgcgcttcgt ccagctgact ggctttcgca cgaatgcgtg
    10561 cttccagctg gtcgatcata tcgttgttgt ccagacggtc tttgcgcacg ccatcttcat
    10621 agaggccgct tttcttgttg ccgccggtga cgccgagtgt agaaaccagc gcctcacctg
    10681 ggccattcac cacgcagccg ataatcgaaa cgtccatcgg agtgatgata tcttccaggc
    10741 gttgctccag cgcgttaacc gtaccgataa catcaaattc ctgacgcgaa caggtcgggc
    10801 aggcgatgaa gttaatccct cgcgaacgga tacgcagcga tttcaaaata tcgaaaccga
    10861 ctttgatctc ttcgaccgga tcggccgcca gcgatacgcg cagcgtgtcg ccgatgcctt
    10921 ccgacagcag cagacctaaa ccaatggcgg attttactgc cccgctgcgt gcaccaccgg
    10981 cttcggtgat ccccagatgc agcggctgat cgatctgttt tgccagcaaa cgataagact
    11041 caacagcgag gaagacgtca gacgctttca cgctgacttt gaactgatcg aagttcaggc
    11101 gatcgagatg atcaacatga cgcatggcgg attccagcaa cgcctgcggc gtcggttcgc
    11161 catacttttc ttgcagatct ttttccagcg atccggcgtt aacgccaata cggatcggaa
    11221 tgtttttatc gcgcgcacag tcaaccacca tgcgaatacg ctcttcatta ccgatattac
    11281 cagggttaat acgcagacaa tcgacgccgt attccgctac tttcagcgca atgcgatagt
    11341 cgaagtggat gtcagccacc agcggcacgt taacccgctg tttgatgagt ttgaacgctt
    11401 ctgccgcgtc catcgtcggt acggagacac ggacgatatc agcgccaacg cgttccagcg
    11461 ccttgatttg attgaccgtt gcttcgacat ctgtcgtacg cgtattggtc atggactgta
    11521 cggcgatggg agcaccatcg ccaatcggca cattcccaac gtaaatacgt gttgattttc
    11581 tacgttgaat tggagcctgg ttatgcatga aaaatctccc acgttacccg tctgttactg
    11641 cgccggtgat tgttcggcat tgagggtcag acgcgcaacc tggttagttc tgataaaacg
    11701 actcagatcg acaggtttcc cttgatactg gatctgtact gcggctggcg caccaatttt
    11761 cagtttatac ggtgcctggc cggttaaatt caaattaccg tctttacgct gcatcccgct
    11821 aaacaatttt ttaccggtag catcagtgac ctccagccag caatcggcag taaagttcat
    11881 caccagcgca ttcggatcag ccgccggcgt ggtcacgcca gcctgatcgg ttggcaacgg
    11941 cgcagcacca tctggcgttg ttgctgccgt tggtaccggg gtcgcggcgg tatcaacatt
    12001 tgcctgcgaa ggcgaaacaa ccgcattctg ttgcggatca acagctggtg ctggcgcagt
    12061 tacggcaggt gtttgcgtgt tggttgcggt agtatccaca gacgctggcg gcgtggacgt
    12121 tgtagccggg tctgtagttg tcgacgtatt taacggaaca ctctgcccct gctcgctatt
    12181 actgctcagt tccgccgaag attgatcggc catagtggtg atctcttcct gctgagcttt
    12241 gtggtcttgc caccaccagg caccgctcag gccgataacc acaaacaaca ccagccaggt
    12301 gaaggtcatc agccagccgt cgcgtttttt gcggcgttta ccgagggaaa aactctgcat
    12361 tggcgcaact tttgcagccc gaagtggagc ctgcttttcc agccctggca gcagttcttc
    12421 ttctggaata tgtaccagac gcgcataaga gcggatatat ccgcgcagga atgttgaagc
    12481 aagatcggcg ggtgccttat cttcttcaat gtcgcgtacc gtggaaacct tcaggcaaag
    12541 tcgctcggca acggcctgct gactaagtcc tagttgttcg cgagcattac gcaggcgagc
    12601 gccggtagta agtgcttcat tttggtcgtg cgtggcttca gtattcattc gctgcaggta
    12661 cgtttaaatg agaattgaga tgccggtgaa ccatcatgct cacccacacc gcgaaacatc
    12721 cgttaagtta accgttatca tacagtataa gaccgtctgt gtgctcgtga caaagcccac
    12781 attcactgag gctaacttac tgttgcatcg ttacatactg ccttaaagtc agcaaaaacg
    12841 caccgttaat attgaccaga caattgcaac ttaatacttc attcataaaa gattacgcca
    12901 cggtacataa agtaaccgtg gcgtaatggc tatcagaccg ctttaatatc gatggcttca
    12961 ccctgcatcc gtttacgcag agtacgtttc gtacggtcga taacatcgcc cgccaactga
    13021 ccacaggcag catcgatatc atcaccacgc gttttacgca caatagtggt gaaaccgtag
    13081 ctcatcagca cttttgagaa acggtcgata cggctgttcg aactgcgtcc atacggcgcg
    13141 cccgggaacg ggttccacgg gatcaggttg atcttacacg gcgtatcttt cagcagttcc
    13201 gccagttggt gcgcgtgttc agtgccgtcg ttaacgtggt caagcatcac gtattcaata
    13261 gtgactcggc cctgattggc gttggatttc tccagataac ggcgcaccgc agcaaggaac
    13321 gtttcgatat tgtacttttt gttgatcggc acaatttcgt cacgaatttc gtcgttcggc
    13381 gcgtgcaggg aaattgccag tgcaacgtcg atcatatcgc ccagtttatc cagcgccgga
    13441 actacaccgg aagtggaaag cgtcacgcga cgtttagaca ggccaaaacc gaaatcatca
    13501 agcatgattt ccatcgccgg aacgacgttg ttcaggttga gcagcggctc gcccatgccc
    13561 atcatcacga cgttagtgat tgggcgctga ccggtgactt ttgctgcgcc gacgattttc
    13621 gccgcacgcc acacctggcc aataatttcc gatacccgca ggttgcggtt aaagccctgc
    13681 tgggcggtgg aacagaattt acactccagc gcacacccca cctgcgaaga gacgcagagc
    13741 gtggcacgat cgtcttccgg gatatacacc gtttcgacgc gctgatcgcc aacggcgatc
    13801 gcccatttaa tggtgccgtc ggatgaacgc tgttcttcaa ccacttccgg tgcgcggatt
    13861 tccgccacct ctttcagttt gccgcgcaac actttgttga tgtcggtcat ctcatcaaag
    13921 ttgtcgcagc aatagtgata catccacttc atcacctgat cggcgcggaa ggttttttca
    13981 cctaaatctt taaaaaactc ccgcatctgc tgacggttga gatccagcag gttgattttt
    14041 ccatctttcg tggtgacgtt ttcaggtgtg actaattgtt cagacatatg ctattccggc
    14101 ctcgttatta cacgttatgg cccctggagg gttgaaaaaa gaaacgcccc ggtgagcggc
    14161 tgctcgtccg ggggcgctgc attgtacaaa ttctggcgca cggatgccac gtttgcacgc
    14221 ggcatttacg aaattattaa cgggtgcgcg ggcacacttc gccttcgccg aagaaataag
    14281 cgatttcgcg agcggcagat tcgacggaat cagaaccgtg ggtaccgttt tcggtcaggc
    14341 tgtcagcgta atcagcgcgc agagtaccag ccagtgcgtt tgccggattg gtcgcgccca
    14401 gcagatcgcg gtgacgctga acggcgtttt caccttccag cacggaaacc acgatcgggc
    14461 cagaggtcat gaattcaacc agaccatcaa agaacggttt tccatcgtgt tcagcataaa
    14521 agccacgtgc ctgttcaacg gtcaggtgca gcattttggt gccgacaatt ttgaaccctg
    14581 cagcttcaaa gcgcgcaaag atattaccaa tgacgttttt tgctaccgcg ttcggtttga
    14641 tgatggaaaa agtacgttca atagccattt ttacctctgt aaattgttct gttgttgtct
    14701 gtaccagcgt acgagatggc gcggattata atgagcaaca gggccgttga ctattgatga
    14761 aggtaacatt tttttaaaat aaaatgagtt ttagcaacaa tcagggacaa aacagactat
    14821 tgcatgacaa atttcactgt cgcgatttgc cccacatcat ccatcaccag caattgataa
    14881 tcacctttat ccgtcaaatg cagggtcacg ttgcgcccgc gttcagttaa cggttcgcca
    14941 ttcagaaacc accagcgttc acctgcccct ccacttgatt gcaacggcaa agtcgcttcc
    15001 gctgcgcccg gtaaacgttt aataatcgcg ccatcgcgaa cgcctgtcag ctgcagaggg
    15061 agctgggcat cgtgaccgta tggcgggcaa attgtcgagg ccggtggtaa gcgcacagcg
    15121 cgacgttctg atgcgggcag ccagggttcc agcggtagcg gccagacatt taccatttct
    15181 tgcttcgcct gcgggcagtc agcggcgacg cgctgccctt tgtcatccag ccagatgggg
    15241 aagcgaatgc cattaatccc ttcctgctct gacagtaaca gcgttggcgg ctggctgcca
    15301 tccagcagcc aggttgccag gcggcggcga cagttaccgt caccttccgg caaagactgt
    15361 ccgcccggcc agcagataac gccacgagtg actgagttcg ggcgcgggtc ttccggcaga
    15421 ttcgcactgc gcgacagtaa gatattattg acctgattca acaatggtac ggcactggca
    15481 aagccaaact gaccaacaac gggcgtgccg tcaggtctgc cagtccagat cccaatgaca
    15541 tagcgagcgt taaccccaat cgcccaggcg tcacgatagc catagctggt gcccgttttc
    15601 catgccagtg gggcgacgcg cggcaaggca ctatccggca agggttgcgc ttcatcagcc
    15661 ataatccggc gaatgatcca cgccgcccct gacgacatta aaggccgttc aagcagcgga
    15721 tcgtcaggct gtaagcgcaa tttgcctgcc ttgccgtggc gagcaaacgc ggtatacgct
    15781 gccgccatat cttccagttt tgcaccagcg ccgccgagaa tgagtgaaag attcggcgca
    15841 gcgccgttgg gcaaatataa cggcaatcca acattgcgta actttgccgc aaaccgtttc
    15901 ggtccatagg cttccagcac ctgcacagca ggtaagttca gcgagcgcac cagcgcctcg
    15961 ctcatgttga tcgggccatg aaaaccgcta tcaaagttac ctagtcgata atcaccggtg
    16021 cgccggggga cgtcttgcag cagtgatgcc gggtggatca agccttcatc cagcgccaga
    16081 ccataaacaa acggtttgag caccgatcct ggcgatcgga tcgcattgac catatcaaca
    16141 tgaccaaagc gtgaatcatc gttgagatca accgatccca cccagccgcg aacacgcata
    16201 tcggtatgat taaccacgat catcgccagt gagctgcgcg gtggcaaccg ccctttccag
    16261 ttttgcgcca gttcttccag acgtcgttga agaccggcat ccagcgtagt aacgattttg
    16321 tcgcttttgc ttttaccgag catcatgcgc gaaaacagcg gtgccagttg cggcatttgt
    16381 cggggtgcca gccagatggg ttcttccctt gactctttta cctgctcacg ggaccacaca
    16441 ccttgcgcag ccatccgttc gagcacttta ttacgcgtat tacgcgcggc ttcggcacgc
    16501 tccggccaac gatccgggcg cagtcggctg ggcgcttgcg gtaacaccgc cagcattgcc
    16561 gcctaggaat agcttaaatt cgcaggcgat tttccgagat aagcccaact tgccgcaccg
    16621 atcccctgca acgtaccgcc aaacggagcg cggttaagat acaagatcag aatttcacgc
    16681 ttagacaaat gccactccag ttgcaacgcg cgccagagct ggagaatttt gccaccaaat
    16741 gttttggggt gaggatcaag cagacgagca acctgcatag tgagcgtgct gccaccggaa
    16801 ataacccgtc ccgaagtgag atcttgccat gctgcgcgcg ccaccgagaa tggattaacc
    16861 cccggatgct tccagaacca gcgatcttca taattgatca gcgcttcaa
//
LOCUS       DBKEKI010000080        16897 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.080, whole genome shotgun sequence.
ACCESSION   DBKEKI010000080 DBKEKI010000000
VERSION     DBKEKI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.080"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACUQPX_002635"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACUQPX_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HHV9057310.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACUQPX_002636"
     CDS             2219..2767
                     /locus_tag="ACUQPX_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HHV9057311.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACUQPX_002637"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACUQPX_002637"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HHV9057312.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACUQPX_002638"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACUQPX_002638"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HHV9057313.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHALDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACUQPX_002639"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACUQPX_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HHV9057314.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="ACUQPX_002640"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="ACUQPX_002640"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HHV9057315.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="ACUQPX_002641"
     CDS             complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="ACUQPX_002641"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HHV9057316.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9767..12379
                     /gene="pepN"
                     /locus_tag="ACUQPX_002642"
     CDS             9767..12379
                     /gene="pepN"
                     /locus_tag="ACUQPX_002642"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HHV9057317.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="ACUQPX_002643"
     CDS             complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="ACUQPX_002643"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HHV9057318.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGPDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13350..14141)
                     /gene="ssuC"
                     /locus_tag="ACUQPX_002644"
                     /pseudo
     CDS             complement(13350..14141)
                     /gene="ssuC"
                     /locus_tag="ACUQPX_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
     gene            complement(14153..15298)
                     /gene="ssuD"
                     /locus_tag="ACUQPX_002645"
     CDS             complement(14153..15298)
                     /gene="ssuD"
                     /locus_tag="ACUQPX_002645"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HHV9057319.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15295..16254)
                     /gene="ssuA"
                     /locus_tag="ACUQPX_002646"
     CDS             complement(15295..16254)
                     /gene="ssuA"
                     /locus_tag="ACUQPX_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HHV9057320.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRTGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16247..16822)
                     /gene="ssuE"
                     /locus_tag="ACUQPX_002647"
     CDS             complement(16247..16822)
                     /gene="ssuE"
                     /locus_tag="ACUQPX_002647"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HHV9057321.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVL
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4215 a         4376 c         4329 g         3977 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgcgatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gagttggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagccca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggcg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgat
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgcaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagagt gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgcgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atggggggga tacctatgtt acctggcatc tgcaatcaga
     8221 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa aagggagccg
     8281 attagctccc tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg
     8341 cagcgcccga acaaacgctt tatcatggca gatagttttg ccagggctgt cagaaagttt
     8401 cgccaccggt ttaccgttac actctaccaa cttaatcaca atattcaggg gttttacctg
     8461 ggggatatcg caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa
     8521 gtggcggtat agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt
     8581 actctgtgga tcaattccca gcttttcata atgtgcaatg gctttttcac cccattcaac
     8641 cgggtcgcca gagtcatgac gcaggccctg ataccgacta gcgaactcga caccgaaatc
     8701 acgcaggaaa gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata
     8761 ctcttccagc caggcagcaa gtgcagctcg ctggctgttg gctagatccg ggctgatttg
     8821 ctgatgtgcc tggaaccatt cgtgtgcctg tgttcccatc ggcgtgaggg aaagccgacg
     8881 cgccagatcg tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gcttaacgat
     8941 ggtttcttgt acttcgcgag aaaaacggcg acgggtgcca aaatccatca gatggaagcg
     9001 cgacatatca agaccggcgg ttaacgccga gaagtcgact aatttgcttt ccagcgtgtc
     9061 gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac
     9121 cgccagcaaa ggaacttccc agaggatgac ttcacgccac ggaccgctta agcgaatatc
     9181 cagcttgcca ttatcgttgg acacggtgac ttgttccggg ttaaagcgga actcgcgtaa
     9241 ccagttaaga tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc
     9301 ctgcaggcgc aggtgctgca tcgcctgaat ctgttcacga atagcatcgg cataaatacc
     9361 cagcagatcg tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg
     9421 aaacacggct tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac
     9481 aggagaagcg aattgtgtca taggtgcgca gtagcgtcct cgtacaggag cgtttagtac
     9541 aataaacatc ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat
     9601 cacaccatat gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag
     9661 caataaaggc gtttgtacct gaaaagatga agattctgca tagcgcgatt tacgcaacag
     9721 gaatagactg aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc
     9781 acaagccaaa taccgtcacg attatcgtgc gccggattac cagattactg atattgactt
     9841 gacctttgac ctcgacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca
     9901 tggtgcatca gatgctccgc ttcgtctcaa cggcgaagac ctcaaactgg tttctgttca
     9961 tattaatgat gagccgtgga ccgcctggaa agaagaagag ggcgcactgg tcatcagtaa
    10021 tttgccggag cgttttacgc ttaagatcat taatgaaata agcccggcgg cgaatacggc
    10081 gctggaaggg ctttatcagt caggcgatgc gctttgcacc cagtgtgaag ccgaaggttt
    10141 ccgccatatt acgtattatc tcgaccgccc ggacgtgctg gcgcgtttta ccaccaaaat
    10201 tattgccgat aaaatcaaat atcccttcct gctttccaat ggtaaccgcg ttgcgcaagg
    10261 cgaactcgaa aacggacgcc attgggtaca gtggcaggac ccgttcccga aaccgtgcta
    10321 cctgtttgcg ctggtggcag gcgactttga tgtactgcgc gataccttta ccacgcgttc
    10381 tggtcgcgaa gtagcactgg agctgtacgt cgatcgcggc aaccttgatc gcgcgccgtg
    10441 ggcgatgacc tcgctgaaaa actccatgaa atgggatgaa gaacgctttg gcctggagta
    10501 tgacctcgac atctatatga tcgtcgcggt ggatttcttc aatatgggcg caatggagaa
    10561 taagggtctg aatatcttta actccaaata tgtgctggcc cgcaccgaca ccgccaccga
    10621 caaagattac ctcgatattg aacgcgttat cggccatgaa tatttccata actggaccgg
    10681 taaccgagtg acctgccgcg actggttcca gctcagtctg aaagaaggtt taaccgtctt
    10741 ccgcgatcag gaattcagct ctgaccttgg ttcccgcgca gttaaccgca tcaataatgt
    10801 gcgcaccatg cgcggattgc agtttgcgga agacgccagc ccgatggcgc acccaatccg
    10861 cccggacatg gtcattgaga tgaacaactt ctacaccctg accgtttacg agaagggcgc
    10921 ggaagtgatc cgtatgatcc acaccctgct gggcgaagaa aacttccaga aagggatgca
    10981 actctatttc gagcgtcatg atggtagcgc agcgacctgt gacgactttg tgcaggcgat
    11041 ggaagatgcg tcgaatgttg atctctccca tttccgccgt tggtacagcc agtccggtac
    11101 gccggttgtg accgtcaaag acgactacaa tccggaaact gagcagtaca ccctgaccat
    11161 cagccagcgc acgccagcca cgccggatca ggcagaaaaa cagccgctgc atattccgtt
    11221 tgccatcgaa ttgtatgaca acgaaggcaa agtgatcccg ttgcagaaag gtggtcatcc
    11281 ggtgaattcc gtgctgaacg tcacccaggc ggaacagacc tttgtttttg ataatgtcta
    11341 cttccagccg gtgcctgcgc tgctgtgcga attctctgcg ccagtgaaac tggaatataa
    11401 gtggagcgat cagcaactga ccttcctgat gcgtcatgcg cgtaatgatt tctcccgttg
    11461 ggatgcggcg caaagtttgc tggcaaccta catcaagctg aacgtcgccc gtcatcagca
    11521 agggcagccg ctgtctctgc cggtacatgt ggctgacgct ttccgcgcgg tgctgctcga
    11581 tgagaagatt gatccggcgc tggcggcaga aatcctgacg ctgccttctg tcaatgaaat
    11641 ggctgaactg ttcgatatca tcgacccgat tgctattgcc gaagtacgcg aagcactcac
    11701 tcgtactctg gcgactgaac tggcggatga gctgctggct atttacaacg cgaattacca
    11761 gagcgagtac cgtgttgagc atgaagatat tgcaaaacgc actctgcgta atgcctgcct
    11821 gcgcttcctt gcttttggtg aaacgcatct ggctgacgtg ctggtgagca agcaatacca
    11881 tgaagcaaac aatatgaccg atgcgctggc agcgctttct gcggcggttg ctgcacagct
    11941 gccttgccgt gacgcgctga tgcaggagta cgacgataag tggcatcagg acggtctggt
    12001 gatggataaa tggtttatcc tgcaagccac cagcccggcg gcgaatgtgc tggagacggt
    12061 gcgtggtctg ttgcagcatc gctcatttac catgagcaac ccgaaccgta ttcgttcgtt
    12121 gattggtgcg tttgcgggca gcaacccggc agcgttccat gccgaagatg gcagcggtta
    12181 ccagttcctg gtggaaatgc ttaccgacct caacagccgt aacccgcagg tagcttcacg
    12241 tctgattgaa ccgctgattc gcctgaaacg ttatgatgcc aaacgtcagg agaaaatgcg
    12301 cgcggcgctg gaacagttga aagggctgga aaatctctct ggcgatcttt acgagaagat
    12361 aactaaagca ctggcttgat acttttgagc caaaatgcct gatgcgacgc ttgacgcgtc
    12421 ttatcaggcc tacaactccg ttgaaattgc tggttttgta ggctggataa ggcgttcacg
    12481 ccgcatcagg cagccgtgcc caactgcctg atgcgatgct tgacgtgtct tatcaggcct
    12541 acaagtccgt tgaaaatgct ggttttgtgc gccacatccg gcatattaac catgacgccg
    12601 aatcagttgt tcagaatgcc cacgccgcat tacccgttgc aatacttccg cttccagctc
    12661 cgctaatcgc accgacccta atcgacgcgg acgggggata tccactgtca aatccggacc
    12721 aatttttccc tcttcaatta acaacacccg gtcagccatt gctaccgctt cgctcacatc
    12781 atgcgtcacc aacagtacgg taaagccgtg ctgctgccaa agtgacacaa tcaaatcctg
    12841 catctcgagt cgcgttaagg cgtccagtgc cccgagcggt tcatcaagca acaataaacc
    12901 cggtcgatga atcaacgctc ttgccagcgc cacacgctgt ttctgcccgc cagaaagtgc
    12961 ggcaggccat tccccggcgc gattctccag tcctaccgca gccaacgccc gacgcgcggc
    13021 atcgcgccac tgacctttaa ggcctaaccc aacgttatca atcaccgatt tccacggcag
    13081 cagacgcgca tcctgaaaca tcattcgcgt atcatcctga atttcagcca gcggtgtggt
    13141 gcccgctaac acatcgcctg cggttggcgt ttccagaccg gccagcaggc gcagcagggt
    13201 acttttacca ccaccgctgc ggcccaccac cgccacaaac tgacctgccg gaatatgtaa
    13261 atccagttgg ttcaggacga tattttccgc gtaatgtttg cttactgcat tgagcaacaa
    13321 tggtgtgccc tggttcagac gagcagtatt catatcgtgg cctccttcaa atgataagcc
    13381 gggttccagc gcaaccagag gcgctctaac agctgcgcgc tgacgtcagc aagtttgccg
    13441 agcagggcgt aaagaataat agcgactacc accacgtctg tttgcaaaaa ctctcgcgca
    13501 ttcatcgcca gataaccaat gccggaattg gcagaaatgg tttcggcaac aatcagcgtc
    13561 agccacatca ggcccaacgc aaaacgcacg ccgaccataa ttgagggcag ggcatcgggc
    13621 aggatcacat ggataaacag cggtatgccg gataatccat agctacgcgc catctccacc
    13681 agcccgcgat cgatattacg gatgccatgc caggtgttga tataaatggg gaacagcgta
    13741 ccgagcgcca ccagaaagat tttcgcggat tcatcaatgc caaaccacaa aatcaccagc
    13801 gggatcagcg ccagatgcgg cacgttgcgc aacatctgaa ttgaggtatc cagcagcctt
    13861 tctccccagc gcgacagtcc gctaatcagt cccagaatca ggcccagcga tccgccaatt
    13921 gaaaagccaa tcagcgcccg ctaggaactg atcgccagat gctgccacag ttcgccgctg
    13981 gcggagagcg tccagaacgc cgtcaccact ccttccggtg aaggcaaaat acgcgtcgac
    14041 agccagccaa ccgaggaggc cagttgccac accgccacga tgcccaccgg taaaaaccag
    14101 ggggcaacgc gcaataacca cttcttcact ggcgttgcca tcatcgtgtt ccttagcttt
    14161 gcgcgacttt acgggggata aaatcattcg ccaccgcttc gccttgcgga ttcagcggct
    14221 gcggctgggg aatttccgga atggcgacat ccagatgtgg gaacagcaac tcgccaaccc
    14281 gatacgcttc ttccagatgc gggtagcccg aaagcacaaa gctgtcgatg ccaagcgcgg
    14341 cgtattcgtt gattcgcgcg gcgaccgtag gaccatcgcc caccagcgcc gttccggcac
    14401 cgccgcgcac taagccaacg cccgcccata aattggggct gatctccaga ttgtcgcgct
    14461 tgccgttatg tagcgccgcc attcgctgtt gtcctacgga atccgtccgg gcgaatgcgg
    14521 cttgtgcttt ggcgatagtc tcatcatcaa gatgcgagat taaccgctcg gcggcctgcc
    14581 acgcttcgtc gttagtttca cgaacaatca catgcagacg aataccgaaa cgaattttgc
    14641 gcccatacgc ggcagctttc gcccgcactt gttcgatttt ctctttaaca agttccggcg
    14701 gttcgcccca ggtgaggtag agatcaactt gttctgccgc cagctcctgg gcgacatctg
    14761 acgatccgcc aaagtaaagc ggaggataag gctgttgaat cggcgggaag agcagttttg
    14821 ctccgcgcac atgaatatgt ttaccgttga aattgacggt ttcccccagc aataagcgcc
    14881 gccagacctg ggtaaattcc gccgaggctt cgtagcgctc gctatgatca aggaacacgc
    14941 cgtcgcctgc cagctcttgt ggatcgctgc ctgtgaccag attaaacaac gcacgaccat
    15001 ttgagagacg gtcaagcgtg gcggcctggc gggcggcaac agtaggtgag gttacgctgg
    15061 gacgcagggc gacaagaaac ttcagccgct gcgtcaccgg gatcatcgac gctgcaacca
    15121 gccacgcatc ttcacacgat cgccccgttg ggatcagcac accggtatag cccagacgat
    15181 ccgccgcttg cgcaatttgt tgcagataac cgtgatcaac cgggcgtgaa ccttcttccg
    15241 ttcccagata gtgcccgtca ccgtgggtcg gtaaaaacca gaacatattc agactcataa
    15301 ttgttttcct tccagttgag tgggctgcca gatgcgctgg cgaatatcga cttttttcgg
    15361 caccagacgg ttttcataaa acagatctgc cgtttgctgc tgtaaggcgg caacctcggc
    15421 gttaaccggt ttgatggtgg taggagggcg atgatctaag taagaggcaa tcaccggtgc
    15481 cggtaagccc atcgtttttg ccagtaaagc gatgctttgt tcgcgctggc tgcgggttaa
    15541 cgcatcggcc tcactaaagg ttgccagtac gccctgaata aaagcgccgt ttttttctgc
    15601 atagggacga gctgccagat aaaacgatcc agtttgattg agatcggtgc cgtctttcag
    15661 cacccgcacg ccgccctgta ataacgcagc ggagtagtag ggatcccaga tagcccaggc
    15721 gtcaacgtta ccttgctgga acgcggcgcg ggcatcggca ggcgtcaggt aagtgggctg
    15781 gatatcggtg aatttaagtc cggcctgacg cagtgcacgc agtaaaaggt tgtgtgaact
    15841 ggaacctttc tgaaaggcaa ctttgtgacc tttaagatcg gctacggttt tgatcgggct
    15901 gttttctgcc accagaatca cttcggcttt gggcttcggt ggctcgacgc ccacatatac
    15961 caaatcagcc ccggcagcct gggcaaagat tggcggaata tccccggtac tgccgagatc
    16021 aatactgcca acgtttaacg cttccaacat ttgcggaccc gcggggaact ccacccagga
    16081 gatttttgat tgcggatagc gtttttccag taactggtgg ctttttgcca gcaccatacc
    16141 aatactgcct ttctgatagc ctgtacgtaa cgcttcaggc gaggattctg cagcaaccgc
    16201 aaaagtagag acgctaagca atcccgccag cgccagttta atgatgttac gcatgggcat
    16261 tacctcgcag agacagaagg tcaggaacct gaacatcgcg gcggtgcaat gcctgccaga
    16321 aagtttctag cgcggtatca agatgggttt gcagatttgg cgtgaactgg ggtctgtgat
    16381 ggtaatcaat taattgtgag tcatcggcaa acacgccgtg caggatctcc tgagctttca
    16441 gtgcgcttaa aactggttta agggcataat cgaccgccag cagatgggcc acggtaccgc
    16501 ccgtcgccag cggtagcaac actttgcctt gcaaagcgcg ctctggcagc aggtcgagca
    16561 gggttttcag cgcgccggaa taggcggctt tatacacagg cgtggcgaca atcagcccat
    16621 cagcctgttg cagctgttcg gtgaaggtct tgagtgccgg actatcgaaa cgagcataaa
    16681 gcagatcttc cggggcgaag ttttgcagat tccagtgata aacctctaca tctaggccat
    16741 ttagtttttc ccgcgcatat tccagcaagg agctggagcg agaaggaaag cgaggactac
    16801 ccgccagggt gatgacacgc atactctctc cttataacca attgttcttt ttttgttaac
    16861 attgataaca attcggtcag tctgtcggag agacaag
//
LOCUS       DBKEKI010000081        16820 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.081, whole genome shotgun sequence.
ACCESSION   DBKEKI010000081 DBKEKI010000000
VERSION     DBKEKI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.081"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..194)
                     /locus_tag="ACUQPX_002648"
     CDS             complement(<1..194)
                     /locus_tag="ACUQPX_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HHV9057322.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVL"
     gene            complement(309..563)
                     /gene="bssS"
                     /locus_tag="ACUQPX_002649"
     CDS             complement(309..563)
                     /gene="bssS"
                     /locus_tag="ACUQPX_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HHV9057323.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            complement(853..1098)
                     /gene="dinI"
                     /locus_tag="ACUQPX_002650"
     CDS             complement(853..1098)
                     /gene="dinI"
                     /locus_tag="ACUQPX_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HHV9057324.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            complement(1172..2218)
                     /gene="pyrC"
                     /locus_tag="ACUQPX_002651"
     CDS             complement(1172..2218)
                     /gene="pyrC"
                     /locus_tag="ACUQPX_002651"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HHV9057325.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAEETVRWSVKQ"
     gene            complement(2324..2884)
                     /locus_tag="ACUQPX_002652"
     CDS             complement(2324..2884)
                     /locus_tag="ACUQPX_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HHV9057326.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            complement(3018..3665)
                     /gene="grxB"
                     /locus_tag="ACUQPX_002653"
     CDS             complement(3018..3665)
                     /gene="grxB"
                     /locus_tag="ACUQPX_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HHV9057327.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNDYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDSLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            complement(3729..4937)
                     /gene="mdtH"
                     /locus_tag="ACUQPX_002654"
     CDS             complement(3729..4937)
                     /gene="mdtH"
                     /locus_tag="ACUQPX_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HHV9057328.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            5173..5757
                     /gene="rimJ"
                     /locus_tag="ACUQPX_002655"
     CDS             5173..5757
                     /gene="rimJ"
                     /locus_tag="ACUQPX_002655"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HHV9057329.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            5768..6415
                     /locus_tag="ACUQPX_002656"
     CDS             5768..6415
                     /locus_tag="ACUQPX_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HHV9057330.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNVVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            6417..7340
                     /locus_tag="ACUQPX_002657"
     CDS             6417..7340
                     /locus_tag="ACUQPX_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HHV9057331.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            7450..8985
                     /gene="murJ"
                     /locus_tag="ACUQPX_002658"
     CDS             7450..8985
                     /gene="murJ"
                     /locus_tag="ACUQPX_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HHV9057332.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(9025..9441)
                     /gene="flgN"
                     /locus_tag="ACUQPX_002659"
     CDS             complement(9025..9441)
                     /gene="flgN"
                     /locus_tag="ACUQPX_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HHV9057333.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMRQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(9446..9739)
                     /gene="flgM"
                     /locus_tag="ACUQPX_002660"
     CDS             complement(9446..9739)
                     /gene="flgM"
                     /locus_tag="ACUQPX_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HHV9057334.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            complement(9815..10474)
                     /gene="flgA"
                     /locus_tag="ACUQPX_002661"
     CDS             complement(9815..10474)
                     /gene="flgA"
                     /locus_tag="ACUQPX_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HHV9057335.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFNAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            10629..11045
                     /gene="flgB"
                     /locus_tag="ACUQPX_002662"
     CDS             10629..11045
                     /gene="flgB"
                     /locus_tag="ACUQPX_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HHV9057336.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            11049..11453
                     /gene="flgC"
                     /locus_tag="ACUQPX_002663"
     CDS             11049..11453
                     /gene="flgC"
                     /locus_tag="ACUQPX_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HHV9057337.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSAPRSYQANVEVLNTVKSMVLKTLTLGQ"
     gene            11465..12160
                     /gene="flgD"
                     /locus_tag="ACUQPX_002664"
     CDS             11465..12160
                     /gene="flgD"
                     /locus_tag="ACUQPX_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HHV9057338.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            12185..13393
                     /gene="flgE"
                     /locus_tag="ACUQPX_002665"
     CDS             12185..13393
                     /gene="flgE"
                     /locus_tag="ACUQPX_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HHV9057339.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            13577..14164
                     /gene="flgF"
                     /locus_tag="ACUQPX_002666"
                     /pseudo
     CDS             13577..14164
                     /gene="flgF"
                     /locus_tag="ACUQPX_002666"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            14302..15084
                     /gene="flgG"
                     /locus_tag="ACUQPX_002667"
     CDS             14302..15084
                     /gene="flgG"
                     /locus_tag="ACUQPX_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HHV9057340.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANVLSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            15137..15835
                     /gene="flgH"
                     /locus_tag="ACUQPX_002668"
     CDS             15137..15835
                     /gene="flgH"
                     /locus_tag="ACUQPX_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HHV9057341.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANVSRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            15847..>16820
                     /gene="flgI"
                     /locus_tag="ACUQPX_002669"
     CDS             15847..>16820
                     /gene="flgI"
                     /locus_tag="ACUQPX_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HHV9057342.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRS"
BASE COUNT         4033 a         4265 c         4477 g         4045 t
ORIGIN      
        1 aggaccaacg ggacatactt ttcgccttca ccataagcat ggcgaattaa tcgcgtatcg
       61 ccgtggtggc tgccgtgttg atgcggtggc atatgggcgt cggtcattag cacgtttaaa
      121 ccggcgcggg ttgcataata cccggcggca gcgcctacgg aaccgctgcc aataatgatg
      181 agatcgtatt tcattttttt ctctctgctc tcatggttta agcagagtaa ttaactgaat
      241 gcagatatac aacccagaaa tggtaaaggc accggtgagg tgccttttgg gtggatggtc
      301 atgtcatgtt aatgacgccg atactcgttt accgggaaat caccggattc aattttggcg
      361 attcctgctt ctaatatcga aataaattgt ctggcaacat cagtggttaa ccagagcgtc
      421 tgaccaactt cagtcccttc ctgctcggac ttatttgggg tctggtagtg caaacgcaac
      481 atcagcgcat catagctatc aacggtgctg atgtcccacc ctacgagcgg atgagtctga
      541 atgacttcat tatttttttc catcatggcc ccctaattcg tgttactaga caacgttatt
      601 cgaggttcaa tgcgttttta tctgaagcaa cttcagtata ccaataagta agggtattca
      661 ccgaattttt aaagggggga acagataaat ttctgctttt aagatttata tgaacaataa
      721 aacagcatgt cattcatatt tttttagcat attgtgcaat tattttgagg aagtgtagaa
      781 attttgtact caaaattcgt aagtaaaata aaaaagccgg ggcgacccgg caaaaacaaa
      841 aatcactgca tattattcgc tgacaaacca gtcatcggcg ctttcccacg tttcctggag
      901 aatttcgcta atgcgctgtt tatcttcttt tgttgcgcca ataaccgata aattattcgc
      961 tgcggcataa cgtaccgata cgtggccttc attatcagga aacgcatact gaatacggcg
     1021 ggaaagttcg ccagccaggg cgtcaatagc cccagctggc aatggagaag ttttcgctat
     1081 ggtgacttca attcgcataa tagccccctg ttgaatatac tggttattta tacaggcaaa
     1141 ataacctaat gacaacagga agctacgatt tttattgttt aacggaccag cgtaccgttt
     1201 cctcggcgag gaatggcacc agggtgtcat cagtcagtgc gatgctttca gcaacctgtt
     1261 gctcttcacg taccagttcg atgaatgtgt cgttgaccgg caagccatag aactgcgggc
     1321 cgtttacaga acagaatgct tcaaagtgct gcagagcatt catctcttca aagacggtag
     1381 cgtaactgcc cagcgcggtt ggggcgttga agcagcccgc gcagccgcaa ctgctctctt
     1441 tgcgatgacg tgcatgtggc gcagaatccg taccgaggaa tactcgatta aaaccgctgg
     1501 cgaccagttc acgcaatgcc tgttggtgaa tattgcgttt gaggatgggt aaacaataca
     1561 ggtgcggacg cacgccacca accagcatat ggttgcggtt aaacatcaga tgctgcggag
     1621 tgatggtggc agccagccgt tcatttccgt cacggacata gtcggcagca tctttggtgg
     1681 tgatgtgctc aaaaacgact ttcagcgcag tcaggcgctg gcgcagaggt tccatcacgc
     1741 tttctataaa gcgcgcttca cgatcaaaaa tgtcgatatc tgcatgtgtc acttcaccat
     1801 gcaccagtag cggcataccg attttttcca tgcgctcaag taccggcatg attgcgtcaa
     1861 ttgacgtcac gccgtggctg gagttagtgg ttgcgtttgc cgggtaaagt tttgcagcgg
     1921 tgaacacgcc ttcattaaat ccgcgctcca gctcattagg atccagcgaa tctgttaaat
     1981 aacaggtcat caatggggtg aaatcgtgcc cggcaggtac ggcgtcaaga atacgttggc
     2041 gatacgccac ggcagcctca actgtggtca cgggcggagc cagattgggc attacgatag
     2101 cccgtccata aatttcgctg gtatacggca cgacagtttt taacatgtcg ccatcgcgga
     2161 ggtgaaggtg ccagtcgtct gggcggcgga tctttaatac ctgggatggt gcagtcatta
     2221 atctctatgc tccggctgaa gggatgtttt tgccggacac aaaggataag cggaaacgtt
     2281 ttcctttgca cgaaaaataa agggcgcgaa tgcgccctcg tcattaatcc gtaaacggaa
     2341 tgacaatttc gcctggcttc acttcaatgc ctttcgccag ttttttcgcc attgcttcgc
     2401 cctgactgcc atcttcgcgc aggacgtaag caggttgctg gttaaagtaa ttgcgtaatg
     2461 cctggttcaa atagggaagc aacgtttgca tcaccgtttg cattttttcc ggttgtaccg
     2521 tcgcatcgac cacttccatc tctttcagga agatcgcacc tttttcttta tcaaacacgg
     2581 gcagcgcttt cagcttcagt ttcatggtcg ctttctgact accgaacagg gagttcatgt
     2641 ccagattggc gtctccggtt agggtaacct tattcggctc ttcgcgacca atttggctgg
     2701 tcaagtttgt cagaacaata tgggcgtcag ccacgccggg taaaccgata tcttttgaga
     2761 aattattatg tttcgcaagc gactggttaa tttcttgttc ggtgatggtg tattgggtga
     2821 gttgattaca gccaacgagc aggccactga cgatcaatgc agcggcaaat aaaaacttgt
     2881 tcatggtagt cctcgacatg aaatctgcgt caatatcctg acacaacgca gcatgtgtca
     2941 ccagcgataa actcgccagc agaaaaaact gaaaacggcg gcaacccgcg aatacaggct
     3001 gccgcggcgg gtcaggatta aatcgccatt gatgataaca aattgatttg tgtctgtttc
     3061 gccatattat cgcggtaatc agcaacgcgg cttggccagt tgattccggc taccagcgtc
     3121 agattacgca gtagcggaaa tagctgaata tcatcttccg aaagttcgcc attcacggcg
     3181 ttcggtttga cgatcagttt gtccagcgca cgcaaatcat cgctgatatt cttaatcaga
     3241 ctgtcagagt gggccagcag gtcggcaaaa ttacccgcgc tggcctcttt cttgtcgacg
     3301 aaatatttgc gcgcggcggg agtagaaaac tcatcaaatg ccgatttggc aaaacgcggc
     3361 aacagcagtt tgttggcgta gtcattgacc ttgcgcagcc actcttcaat ggcaggggaa
     3421 cgtttgccgg tcagtaacgg tttgccgtcg agtttatcga catagtgaac gatatccatg
     3481 ctttctggca tatagcggct gtcatctttt tgcagaatgg gaacctgttt ttgaccgacc
     3541 atccgggtgg gtgtttctgc gtcgtcgttg agcagaacat gtaattcgac ggggatattt
     3601 ttcaggccga aaatcatgcg ggctttgagg cagtaagggc agtgatcgta aatgtatagc
     3661 ttcacgtgac tcctccattt ggctgtcagt tcactttcag tatggaggag tcagggggga
     3721 gtatcaaatc aggcgtcgcg ttccagcaaa cgacgcgcgg cgcgtttctg actaaactgc
     3781 caacccagcg caaggaaagt gaagatgcca ataattccca gcatcatcca cggaagctct
     3841 ggctggtgcg ccgatttgcc caggtcaaac agccagccac caccgatata accaatagcg
     3901 ccgccaatcg ccagacccag acggctaaac cccatatagc tgccgcgagc tcttgcgtcc
     3961 gccagcgaag cacttaaggt ttcacgcgca ggctcggcaa tgatcgaccc gatataaaac
     4021 agacaaatca gggtgaaaag ttgttgcagg ccgctgacca tgcccaccgg catcatgctt
     4081 aatgacatta tcaacagccc agccatcaac cggtgttcca gacgaaaatg cttttcactc
     4141 cagcgggcga tagggtagag caacgttaac gacagacacg cttcaatggc atacatccat
     4201 ttaacggcag agggcgcgcc agccacgtcg ttgaccataa ttggcagcat cagcatcact
     4261 tgtacagcca gcatgtagta acccgccagc gtcaggacat aggtgacaaa acgcttgtca
     4321 cgcatcacgc gggtcatgcc ttcgcgaacg ggcgtgcgta cggtggagag tttccatgcc
     4381 ggtaacaacc acgcattgaa cgccgcacat agcacaaaca aaacggcccc tgtggcgcag
     4441 accaggcgaa agtcgtattg caacagccag ctccccaaca atgcgccaat gaccgcaccg
     4501 gcactgtcct gcatcatcaa cagcgagaaa aaacgaccac gttgttgtgg acggattagt
     4561 ttcaccacca gcgccgaacg cggcggatca aacaacgtgc cgccgagtcc cgagagcagg
     4621 catgaaaacc acaatagcca cggttcgtgg gcgataccca ttgtggcgaa tccggcggcg
     4681 cgcatcagca taccggtaac aatcatcggt ttggcaccaa agcggtcggc aattgcgccg
     4741 ccgaaaatac ccagaccttg ctgaataaac tggcgtagac cgagagcaat accgaccatg
     4801 acggcggccc agcccatttg atcaacgaag cggatagaga tcagcgggaa gacaacaaag
     4861 aaccccagca cgaccagcat attatcgatg agcaggaaat atttacccag gttcctcgcc
     4921 tgcgatacgc gggacatttc ccctcccggg aaataaaaga tgagcacttt ctattctgca
     4981 ttgccgctaa gacttttccc atcacttcgg ggacaatatt tttttatcaa aacgtcgctt
     5041 tgatagagag tttttatcaa atatgtgaat aatgcagaaa atggcatttt ggacttttca
     5101 cagggtctgg ttgcgcaggt atagtaatac ttacagcgta ttaaagacgt tacgggaagg
     5161 agtaggtata gaatgtttgg ctatcgcagt aacgtgccaa aagtgcgctt aaccacagac
     5221 cgactggtcg tgcgtctggt gcatgatcgt gatgcctggc gtctggcgga ttattacgca
     5281 gagaatcgcc atttcctcaa gccctgggag ccagtgcgcg acgaaagcca ctgttatcca
     5341 tcaggctggc aggccaggct agggatgatt aacgaatttc ataaacaagg ttcagctttc
     5401 tactttggct tattcgaccc ggacgaaaaa gagattattg gcgttgccaa tttttccaat
     5461 gttgttcgcg gctcttttca tgcctgctat ctcggttatt cgattgggca aaaatggcag
     5521 ggcaaaggac tcatgtttga agccctgacc gcagccattc gttatatgca gcgcacccaa
     5581 catattcatc gcattatggc taattatatg ccgcacaata aacgcagcgg tgatttactg
     5641 gcgcgactgg gttttgaaaa agaaggctat gcgaaagact atctgttgat tgatggacaa
     5701 tggcgcgatc acgtactgac ggcattaact accccagact ggacgcccgg ccgctaagga
     5761 gaatctcatg aaatatcaac ttactgccct ggaagctcgc gtaattggtt gcttgctgga
     5821 aaagcaggtg accacgccgg agcaataccc gctctcagtc aatggtgtag tcacggcctg
     5881 taatcagaaa acgaaccgtg aaccggtcat gaatctgagt gaatccgaag tgcaggaaca
     5941 gctggataat ctggtcaaac gtcattatct acgcacagtg agcggttttg gtaatcgggt
     6001 caccaaatat gagcaacgtt tttgtaattc agaatttggc gatctgaaac tgagcgccgc
     6061 agaagtggcg ttaatcacca cgttgttatt gcgtggtgcc cagacgccgg gtgaactgcg
     6121 cagccgcgcc gcgcgaatgt atgaattcag cgatatggcg gaagtggagt cgacgctgga
     6181 acaactggcg aatcgcgaag atggtccttt tgtggtgcgt ctggcccgcg aaccgggtaa
     6241 acgcgaaagc cgctacatgc atcttttcag tggtgaggtt gaagatcagc cggcggtaac
     6301 ggctatgtcg aacgtggttg acggtgattt acagacccgc gtcgaagccc tggaaatcga
     6361 agtggcagaa ctgaaacaac gtcttgattc gttgctggcc catctgggag attaaagtga
     6421 aaaaattacg tatcggcgta gtgggattag gcggcattgc gcaaaaagcg tggttaccgg
     6481 tgctggcggc agcgtctgac tggacgttac aaggagcctg gtcgcctacg cgcgccaaag
     6541 ccctgccaat ttgtgacagc tggcgcattc cttatgccga ttcgttatcc agccttgccg
     6601 ccagttgtga tgcggttttt gtgcactcca gcaccgccag ccactttgac gtggtcagta
     6661 cgttactcaa tgcgggggta catgtctgtg tcgataaacc gctggcagaa aatctgcgcg
     6721 atgctgaaag gctggtggaa ctggcggcgc ggaaaaaact gacgttgatg gtcggtttta
     6781 accgtcgttt cgcaccactc tacggtgagt taaaaacgca actcgccacc gcagcctcgc
     6841 taagaatgga taaacatcgt agcaatagcg tcgggccaca cgatctttat ttcacgttgc
     6901 tggatgatta tctgcatgtg gtggataccg cgctgtggtt gtcgggcggc aaagcctctc
     6961 tggatggcgg tacgctactg actaacgacg ctggcgaaat gctgtttgcc gagcaccatt
     7021 tttcggccgg tcctttgcag atcaccactt gtatgcatcg ccgtgccgga agtcagcgtg
     7081 aaaccgtgca ggccgtgact gacggtgcgc tcatcgacat tacggatatg cgcgaatggc
     7141 gtgaggagcg cgggcagggt gtagtgaata aaccgattcc tggttggcag agcactcttg
     7201 agcaacgtgg atttgtcggc tgtgcgcggc acttcattga atgcgtgcaa aatcagacag
     7261 ttccgcaaac cgccggcgaa caggcagtgc tggcgcaacg tatcgttgac aagatctggc
     7321 gcgatgcgat gagtgaataa accctgtaac atctggcggt agcaattcac cataatccag
     7381 gtagactatt cgcctctttc agcgcctgcc ttgcaggcgt tttgcccgtg ggtccggatt
     7441 agaacaccga tgaatttatt aaaatcgctg gccgccgtca gctcgatgac catgttttcg
     7501 cgtgtgcttg gcttcgcacg agacgcaatt gtcgccagaa tctttggcgc agggatggca
     7561 accgacgcct ttttcgtcgc ttttaaactt cctaacttgt tacgccgtat ctttgccgaa
     7621 ggggcatttt cccaggcatt tgtaccgatt ctggcggaat ataaaagtaa gcagggtgaa
     7681 gacgccacgc gggtctttgt ctcttatgtt tctggcctgc tgacacttgc gctggcggtt
     7741 gtgacggtcg ctggcatgct cgccgcaccg tgggtgatca tggtgaccgc gccaggcttc
     7801 gctgacacag ctgacaaatt tgccctgacc agccagctac taaagattac ctttccctat
     7861 atcttgctga tctccctggc gtcgctggtg ggagcgattc tgaatacgtg gaaccgcttc
     7921 tcgattccgg cgtttgcgcc aacactgctt aacatcagca tgattggttt cgcgctgttt
     7981 gccgcaccgt actttaaccc accggtgctg gcgctggcgt gggctgttac ggtcggcggc
     8041 atcctgcagc tggtgtatca gctaccgcac ctgaagaaga tcggcatgct ggtcctgccg
     8101 cgcattaact tccacgatgc cggagcaatg cgcgtggtga aacagatggg gccggcgatc
     8161 cttggcgtct ctgtgagcca gatctcctta atcatcaaca ccatttttgc ctcgtttctt
     8221 gcttccggtt cggtgtcttg gatgtattac gccgaccgct taatggagtt tccctccggt
     8281 gtgctgggcg tggcgcttgg caccattttg ctgccgtcgc tgtcgaaaag ttttgccagt
     8341 ggcaatcatg atgaatacaa ccgtttgatg gactgggggt tgcgtctttg tttcctgttg
     8401 gcactgccga gtgcggttgc gttgggcatt ctttcaggtc cgttaaccgt ttcgctgttc
     8461 cagtacggta aatttaccgc gtttgatgcg ctgatgaccc agcgggcgtt aattgcctac
     8521 tcggtgggtt tgattggcct gattgtagtg aaagtgttgg ctcctggctt ttattcccgc
     8581 caggacatta aaacgccagt gaaaattgcc atcgttacgc tgattttaac gcaattgatg
     8641 aacctggcgt ttattggtcc gttgaaacat gccgggctgt cactttctat tggtctggcg
     8701 gcgtgtctga atgcttcgct gctttactgg cagttgcgta agcagaaaat ctttactccg
     8761 caacccggct ggatggcgtt tctgttgcgt ctggtggtgg cggtactggt gatgtctggc
     8821 gtgcttttag gtatgttaca tatcatgccg gagtggtcat tgggtaccat gccctggcgc
     8881 ttactgcgtt taatggcggt cgtgctggcg gggattgccg cgtacttcgc tgcactggcg
     8941 gtactgggct tcaaagttaa agaatttgcc cgccggacgg tgtaacaatg cattccggcc
     9001 tgcagtgcag gccggagata atattcagat cgaaatcttt ttactgccgc gatggggtgt
     9061 tgaggtctga ccgttcgccc catatagcgt cggttcctga tgcggtttca acatttccag
     9121 cgcctgttga ttgcgctcaa tctgtccttc cagtaaccag ccgttatgtt gattcatttg
     9181 gcgtagttgc tgcgttttca cagtaatttc ctgccagcgt tgactaatat caacgctatt
     9241 tgctgtattg ggttctttct tgcgtaactg ttcgaggtaa tccagcgtcg ccagcagtga
     9301 gcttttttgt tctgtaatcc attgcaactg gctgccgttg atctgcctca tagagagatg
     9361 ttgctgctct tgatccatta ccgttttgag atcgttaagc acagcggaca tctggtcgag
     9421 gatctctgca agacgtgtca tacgatcagt tactctgcaa gtcttgctgc gcttcgttga
     9481 tcagcgcatc ggcaattttg ccggtgtcca tttttagttc accgttacga atcgccagtt
     9541 ttaacgcttc gacacgttca agattgatat cactgctgcc gggttgcatc agttttgctt
     9601 gcgcgtcgct taacgtcaca ctggtgctgg tggaggcggt tgtttttgcc gcccggctgt
     9661 tcgttaccgg cgcgtcagtg gtttcgcgcg gttgaacggt gcttacaggc ttcagaggcg
     9721 aagtgcgatc aatactcatg gtttattcct cattgagggc gcttttatca tgtgttgctt
     9781 atttatcggc aagggacggg taatctttaa cagcttacag gtttataaga atattcccat
     9841 ctgcatcaac aacgccgctg actacctgtc ccgataccat gcgcacccgc gcattctgtg
     9901 cgacggctgc attgttcagc gcctgacctt ctgcgttggc gctaaaccca tcaccgctgg
     9961 cgatcacatt gacgcgttgt cccgctttta cccgccatgc ctggcgaaac tgggttaact
    10021 ggataggttg atcgggtgat agatcgcgca ggctaacggc atcaacaagt tgattgatat
    10081 ccagcaccgt acgcggtggc agggtatcca gccgtccacg tttcagtttg acattgccag
    10141 cttccagctt tcctccccgc acaataggca tcgcggcaac cacataattt cctgtggcct
    10201 gcacattaac ctgtaaatat cgtttgtcgt taccgcagcg tgccaacaca ttcacattgc
    10261 cccacaggcg ggaattattg ctcatcgaaa gcaatggctg ctcgcatggc ggtagtagat
    10321 tgggcgctgt acgaatagaa acacgaacct catcacttac ccccacgagt tgggcgttaa
    10381 aaaagttgtg caattgcgat gtgagattgc tcgccgtact taacggactg aacagtatcg
    10441 cgatgatcgc cacgctacgt tttattgtca gcattttcgc ccccagccat ttctacaacg
    10501 tgaattgtac ctgtccgcaa tgaccatcaa cggcataaat agcgacccat tttgcgttta
    10561 ttccgccgat aacgcgcgcg taaaggcatt taagctgatg gcagaatttt gatacctgcg
    10621 gaggagatat gctcgataag ctcgacgccg ccttacgttt tcaacaagag gcgctcaatc
    10681 tgcgcgccca gcgtcaggaa gtgctggcag caaacatcgc caatgccgat acccctggtt
    10741 atcaggcgcg cgatatcgat tttgccagtg aacttaaaaa agtcatgcaa cgtggacggg
    10801 atgcaaccag tgtggttgca ctgacgatga cctcaacgca acacatcccg gcgcaggcgc
    10861 tgacgcctcc taccgcagaa ctgcaatacc gtattccgga ccagccttcg cttgacggta
    10921 ataccgtcga tatggatcgc gaacgcaccc agtttgccga taacagcctg caataccaga
    10981 tgagccttag cgcgttgagc gggcaaatca aaggcatgat gaacgtttta cagagcggaa
    11041 attaacggat ggcactgctg aatatttttg atatcgccgg gtcggcgtta actgcccagt
    11101 cccagcgcct gaacgtggcg gccagtaatc tggcaaatgc tgatagcgtg accggtcctg
    11161 atggacagcc atatcgggca aaacaggtgg tattccaggt taacgctgca ccaggtgctg
    11221 cgacaggcgg cgtaaaggtt gccgatgtta tagaaagtca ggccccggac aaactggttt
    11281 atgaaccagg taatccgctg gcagatgcaa agggctacgt aaaaatgccg aacgttgatg
    11341 tcgtcgggga gatggttaac accatgtcgg cgccacgcag ctatcaggcc aatgttgaag
    11401 tgctcaacac ggtgaaaagc atggtgctga aaacccttac gctcggtcaa taaaggagaa
    11461 agctatgtcc attgcggtaa ccaccaccga tccgacaaat accggcgtca gtaccaccag
    11521 cagtagttcg ctcacgggca gcaacgccgc agatttacaa agcagttttc tgactttgct
    11581 ggtggcgcag ctgaaaaacc aggacccgac caatccaatg gaaaacaacg agctgacgtc
    11641 gcaattggca caaatcagca cggtcagcgg gattgaaaaa ctcaatacca cgctcggatc
    11701 tatttccgga cagattgata acagccagtc gttacaggcc agtaacctga tcggtcacgg
    11761 cgtgatgatc cccggcacaa ctgttcttgc gggaaccggc agtgaagaag gggctgtgac
    11821 cacgaccacg ccgtttggtg ttgagctgca acaggcggca gacaaagtta cggccaccat
    11881 caccgataaa aatggcgcgg ttgtgcgcac cattgatatt ggtgaactga ccgccggagt
    11941 tcacagtttc acctgggacg gtacgttgac tgatggcagc actgcgccga acggttctta
    12001 caatgtagcg attagcgcca gtaacggtgg tacacaactg gttgcccagc cgctgcagtt
    12061 tgctctggtg cagggtgtga tccgcggcaa cagcggtaat acgctggatc tcggcactta
    12121 cggcaccacc accctcgacg aagtacggca gataatttaa gccttcacat ttcaggagtc
    12181 agtcatggcc ttttctcaag cggttagcgg attaaacgct gccgccacca acctcgatgt
    12241 tattggcaac aatatcgcca actccgccac ctacggcttt aaatcaggca cggcctcttt
    12301 tgccgatatg tttgccggtt cgaaagtggg actgggggta aaagttgccg gtatcactca
    12361 ggactttacc gatggcacga ccaccaacac cgggcgaggt ctggacgttg ctatcagcca
    12421 gaacggtttt ttccgtctgg tagacagcaa cggttcggtg ttctacagcc gtaacggaca
    12481 atttaagctg gatgaaaacc gtaacctggt gaatatgcaa ggtttacagc tgacgggtta
    12541 cccggcaacc ggtacgccgc cgactattca gcaaggggcg aatccgacca atatttcgat
    12601 cccgaatacc ctgatggcag cgaaaactac caccacggcg tcgatgcaga tcaacctgaa
    12661 ttccagtgat ccgcttccta ctgttacgcc attcagcgcc agcaatgcgg atagctataa
    12721 caaaaaaggt tcggtgactg ttttcgacag tcagggtaat gctcatgaca tgagcgtcta
    12781 ttttgtgaag accggggata ataactggca ggtctacacc caggatagca gtgatccaaa
    12841 cagcattgcg aagacagcga caacactgga atttaatgct aatggcacat tagtggatgg
    12901 tgcgatggcg aataatatcg caaccggcgc aattaacggt gcagaacccg ccacgtttag
    12961 tctgagcttc ctcaactcca tgcagcaaaa taccggcgct aacaacattg tggcaaccac
    13021 ccagaatggc tacaaaccgg gcgatctggt gagttatcaa atcaatgatg acggtacggt
    13081 tgtcggcaac tattccaacg aacaaaccca actgctgggg cagattgtac tggcgaactt
    13141 tgccaacaac gaaggtctgg catccgaagg cgacaacgtc tggtctgcga cgcaatcttc
    13201 tggcgtggcg ctgttgggga cagccgggac gggaaacttt ggcaccctga ccaacggtgc
    13261 gctggaagcg tccaacgtcg atctcagtaa agaactggtc aatatgatcg ttgcccagcg
    13321 taactatcag tctaacgccc agaccatcaa aacccaggac cagatcctca acacgctggt
    13381 taacttacgc taatcgctga cgggatagct caatggatca cgcaatttat actgcgatgg
    13441 gagcagccag ccagacgctg aatcaacagg cggtaaccgc cagtaatctg gccaatgcct
    13501 ctacgcccgg ttttcgcgcg cagttaaacg ccttgcgcgc agtgccggtg gaagggcttt
    13561 cactgcccac gcgcacgctg gtaacggcgt caacaccggg tgcagacatg acgcccggca
    13621 agatggatta cacctcgtgc ccgctggacg ttgcgttgca gcaggatggc tggccgtgca
    13681 gaccgctgac ggcagcgaag ggtatacgcg taatggcagc attcaggttg atcccaccgg
    13741 gcaactgaca attcaggggt atccggtgat aggcgaggcg gggccaattg ctgtgccgga
    13801 aggggcggaa atcacgattg ctgccgatgg cacaatctcg gcgctcaatc cgggcgatcc
    13861 ggcaaataca gttgcgccag tagggcgtct gaaactgatg aaagccacgg gcagcgaagt
    13921 gcagcgcggt gacgacggca tttttcgttt aagcgcagaa acccaggcca cgcgagggcc
    13981 ggtactgcag gcagatccaa ccttgcgtgt gatgtcgggg gttctggaag gcagtaacgt
    14041 caatgccgtt gcggcaatga gcgacatgat tgccagcgcg cggcgttttg aaatgcagat
    14101 gaaggtgatc agcagcgtcg atgataacgc aggccgtgcc aaccaactgc tgtcgatgag
    14161 ttaattgaac gattgatgac attgcaataa aagcgcgcgg actattccct cgcccctttg
    14221 gggagagggt tagggtgagg ggaaattcac actgtcgcgt gcttctgctc tgcttgtcag
    14281 caatccacta caggacatta tatgatcagt tcattatgga tcgccaaaac gggccttgac
    14341 gcccagcaaa ccaatatgga cgtcattgcc aacaacctgg caaacgtcag tactaacggt
    14401 tttaagcgtc agcgcgcggt gtttgaagat ctgctttatc aaaccattcg ccagccgggg
    14461 gcacagtctt ccgaacaaac caccttaccc tccggattac aaatcggcac gggggtacgc
    14521 ccggtcgcca ctgaacgctt acacagccag ggcaatctgt cgcagaccaa taacagcaaa
    14581 gatgtcgcga ttaaagggca gggctttttc caggtaatgt tgccagatgg ctcatcagcc
    14641 tatacccgtg acggctcttt ccaggtggat cagaacgggc agctggtgac ggctggtggt
    14701 tttcaggtgc agccagcgat caccattccg gcgaatgtgt taagtatcac catcggtcgt
    14761 gatggcgtgg tcagcgtaac ccaacaaggc caggcagctc cggttcaggt tgggcagctc
    14821 aatctcacca cctttatgaa tgataccgga ctggagagca ttggcgaaaa cctctacacc
    14881 gaaacgcaat cctccggtgc accgaacgaa agcacgccgg gcctgaacgg cgcgggactg
    14941 ctgtatcaag ggtatgttga aacgtctaac gtcaacgtgg cggaagaact ggtcaatatg
    15001 attcaggtgc aacgcgctta cgaaatcaac agtaaagcgg tgtccaccac cgatcagatg
    15061 ctgcaaaaac tgacgcaact ctaaggcata accggtggca ggttcaccgg tttactgatt
    15121 tttgaagatg atagccatgc aaaaaaacgc tgcgcatact tatgccattt ccagcttgtt
    15181 ggtgctttca ctaaccggct gcgcctggat accctccacg ccgctggtgc agggggcgac
    15241 cagtgcacaa ccggttcccg gtccgacgcc cgtcgccaac ggttctattt tccagtctgc
    15301 tcagccgatt aactatggct atcaaccgct gtttgaagat cgtcgaccac gcaatattgg
    15361 cgatacgctg accatcgtgt tgcaggagaa cgtcagcgcc agcaaaagct cctctgcgaa
    15421 tgtcagccgt gacggtaaaa ctaattttgg ctttgatact gtgccgcgct atttgcaggg
    15481 gctgtttggt aacgctcgtg ccgatgtcga agcctccggt ggtaacacgt tcaacggaaa
    15541 gggcggggcc aatgccagca atacctttag cggcacgttg acggtgacgg ttgaccaggt
    15601 actggtcaac ggcaacctgc atgtggtggg tgaaaaacag attgccatta atcagggtac
    15661 cgaatttatt cgcttctcgg gcgtggttaa tccacgcact atcagcggca gcaataccgt
    15721 accgtctact caggtggcgg atgcgcgtat tgaatacgta ggcaatggct acattaacga
    15781 agcgcaaaat atgggctggt tgcagcgttt cttccttaac ctgtcgccaa tgtaagtgag
    15841 gctgtggtga ttaaatttct ctctgcatta attcttctac tggttacgac ggcggctcag
    15901 gctgagcgta ttcgcgatct caccagtgtt cagggggtaa ggcaaaactc actgattggc
    15961 tatggcctgg tagtggggct ggatggcacc ggtgaccaga caacccagac gccgtttacc
    16021 acacaaacgc ttaataacat gctctcacag ctgggaatta ccgttccgac gggcaccaat
    16081 atgcagctaa aaaacgtcgc tgcggtaatg gtgacagcgt cacttcctcc gtttggacgt
    16141 caggggcaaa ccatcgatgt ggtggtttct tccatgggta atgccaaaag cttgcgtgga
    16201 ggtacgttgt tgatgacacc gcttaagggc gttgacagtc aggtgtatgc gctggcgcag
    16261 ggcaatattc tggttggcgg cgcaggagcc tccgcgggcg gtagcagtgt tcaggttaac
    16321 caactgaacg gtggacggat caccaatggt gcggttattg aacgtgaatt gcccagccag
    16381 tttggcgtcg ggaataccct taatttgcaa cttaacgacg aagatttcag catggcgcag
    16441 caaatcgctg acaccatcaa ccgcgtgcgt ggatatggca gcgccaccgc gttagatgcg
    16501 cggactattc aggtgcgcgt accaagtggc aacagttccc aggtccgttt ccttgccgat
    16561 atccagaata tgcaggttaa tgtcaccccg caggacgcta aagtagtgat taactcgcgc
    16621 accggttcgg tggtgatgaa tcgcgaagtg accctcgaca gctgcgcggt agcgcaggga
    16681 aatctctcag taacagttaa tcgtcaggcc aatgtcagcc agccagatac accgtttggt
    16741 ggtggacaga ctgttgttac tccacaaacg cagatcgatt tacgccagag cggcggttcg
    16801 ctgcaaagcg tacgttccag
//
LOCUS       DBKEKI010000082        16500 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.082, whole genome shotgun sequence.
ACCESSION   DBKEKI010000082 DBKEKI010000000
VERSION     DBKEKI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.082"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1312
                     /locus_tag="ACUQPX_002670"
     CDS             <1..1312
                     /locus_tag="ACUQPX_002670"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9057343.1"
                     /translation="DKVKYPTGVENHGGTLRIWFNFKGKRVRENLGVPDTAKNRKIAG
                     ELRTSVCFAIRTGNFDYAAQFPDSPNLKAFGVNKKEITVKELEEKWLDLKRMEISANA
                     FNRYESIARMMVPKIGGNRLVSAVTKEELLYIRKDLLTGYQNPTKGKAPVKGRSVVTV
                     NYYMTTIAGMFQFAADHGYIEANPFEGIKPLKRARAEPDPLTRDEFIRLIDACRHQQT
                     KNLWSLAVYTGMRHGELVSLAWEDIDLKAGTITIRRNYTKLGEFTLPKTEASTNRVVH
                     LIQPAISVLKNQAEMTRLGKQHNIKVQLREYGRSVNHECTFVFNPQVVRKSKQVGFIY
                     KVDSIGDSWETAIKRAGIRHRKAYQSRHTYACWSLSAGANPSFIASQMGHASAQMVFN
                     VYGAWMTDSNAEQIAMLNQKLADYVPMMSHGHQSDTRGLLKSVS"
     gene            1294..2064
                     /locus_tag="ACUQPX_002671"
     CDS             1294..2064
                     /locus_tag="ACUQPX_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HHV9057344.1"
                     /translation="MKISELAFNTRHVNCVEGNTTLYALPKPEVVLRWHEQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPAEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            2117..2512
                     /locus_tag="ACUQPX_002672"
     CDS             2117..2512
                     /locus_tag="ACUQPX_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HHV9057345.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2553..3296
                     /gene="cmoA"
                     /locus_tag="ACUQPX_002673"
     CDS             2553..3296
                     /gene="cmoA"
                     /locus_tag="ACUQPX_002673"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HHV9057346.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3293..4264
                     /gene="cmoB"
                     /locus_tag="ACUQPX_002674"
     CDS             3293..4264
                     /gene="cmoB"
                     /locus_tag="ACUQPX_002674"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HHV9057347.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4429..6858)
                     /gene="torZ"
                     /locus_tag="ACUQPX_002675"
     CDS             complement(4429..6858)
                     /gene="torZ"
                     /locus_tag="ACUQPX_002675"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HHV9057348.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKNIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNTL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKSAVQA"
     gene            complement(6883..7983)
                     /locus_tag="ACUQPX_002676"
     CDS             complement(6883..7983)
                     /locus_tag="ACUQPX_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HHV9057349.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8371..9117)
                     /gene="cutC"
                     /locus_tag="ACUQPX_002677"
     CDS             complement(8371..9117)
                     /gene="cutC"
                     /locus_tag="ACUQPX_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HHV9057350.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYCDGEFAAILEDVRTVRELSFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGTWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(9131..9697)
                     /locus_tag="ACUQPX_002678"
     CDS             complement(9131..9697)
                     /locus_tag="ACUQPX_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HHV9057351.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9913..11646
                     /gene="argS"
                     /locus_tag="ACUQPX_002679"
     CDS             9913..11646
                     /gene="argS"
                     /locus_tag="ACUQPX_002679"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HHV9057352.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAAYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(11688..11732)
                     /locus_tag="ACUQPX_002680"
     CDS             complement(11688..11732)
                     /locus_tag="ACUQPX_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057353.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            11823..12311
                     /locus_tag="ACUQPX_002681"
                     /pseudo
     CDS             11823..12311
                     /locus_tag="ACUQPX_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(12429..12821)
                     /gene="flhE"
                     /locus_tag="ACUQPX_002682"
     CDS             complement(12429..12821)
                     /gene="flhE"
                     /locus_tag="ACUQPX_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HHV9057354.1"
                     /translation="MRILLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(12821..14838)
                     /gene="flhA"
                     /locus_tag="ACUQPX_002683"
                     /pseudo
     CDS             complement(12821..14838)
                     /gene="flhA"
                     /locus_tag="ACUQPX_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(14891..16038)
                     /gene="flhB"
                     /locus_tag="ACUQPX_002684"
                     /pseudo
     CDS             complement(14891..16038)
                     /gene="flhB"
                     /locus_tag="ACUQPX_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            16274..>16500
                     /locus_tag="ACUQPX_002685"
     CDS             16274..>16500
                     /locus_tag="ACUQPX_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HHV9057355.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVA"
BASE COUNT         4137 a         4304 c         3966 g         4093 t
ORIGIN      
        1 ggataaagtc aaatatccaa caggcgtcga aaaccacggt ggcacattac gcatctggtt
       61 taattttaaa ggtaaacgtg tcagggaaaa tcttggtgtc cctgacactg ccaagaacag
      121 gaagatcgcc ggagagctgc ggacatctgt atgttttgcc attcgtacag gaaattttga
      181 ttatgctgcg cagttccctg actctcctaa cctcaaggct tttggggtaa ataaaaaaga
      241 aattacggtg aaagaacttg aagaaaaatg gctggatctg aaacgaatgg aaatctctgc
      301 aaatgcattc aatcgctatg aatccattgc aaggatgatg gttccgaaga ttggtggcaa
      361 ccgactggta tcagcagtga ccaaagagga attgctgtat atcaggaaag atttgctgac
      421 cggataccaa aatccaacaa aaggtaaagc tccagtaaaa ggacgaagcg ttgtcacagt
      481 aaattattac atgacaacaa tcgctggaat gtttcagttt gctgcagatc acggttacat
      541 agaagcaaat cccttcgagg gaattaagcc tcttaaaaga gccagggcag aaccagatcc
      601 gctaactcgt gacgaattta ttcgcctgat agatgcttgc cggcatcagc agacaaaaaa
      661 cctgtggtca ttggctgtgt acacaggaat gcgtcatggt gaactggtct ccctggcctg
      721 ggaagatatc gatctgaaag caggaacaat tactatcagg cgcaattata cgaaactcgg
      781 tgagttcact ctacctaaaa ctgaagcaag tacaaacagg gttgtgcatc ttatccagcc
      841 cgctatcagt gtcctgaaaa atcaggctga aatgacaaga ctgggtaagc agcacaacat
      901 caaggttcaa ctacgtgaat atggacgttc agtaaatcat gaatgtactt tcgtatttaa
      961 cccccaggtg gttagaaaaa gcaaacaggt aggttttatc tacaaggtag attccattgg
     1021 cgactcatgg gaaacagcca ttaaacgtgc aggcatcagg catcgaaagg cataccagtc
     1081 acgacacact tatgcgtgct ggtcattatc tgccggagca aatccaagct tcattgccag
     1141 ccagatgggg catgcaagtg cccagatggt gttcaatgta tacggagcat ggatgactga
     1201 cagcaatgca gaacagatcg caatgctgaa tcagaagctg gcagattatg tcccaatgat
     1261 gtcccatggt caccaaagtg acacaagagg cttattaaaa tcagtgagtt agcttttaat
     1321 acccgtcatg ttaactgcgt ggagggcaac accacgcttt acgccctgcc gaaacccgag
     1381 gttgtcctgc gctggcatga acagaccaca gatgacttcc gcttctgttt taagtttccg
     1441 gcgaccattt cgcatcaggc agcattacga cattgcgatg atttagtgac tgaatttttg
     1501 acccgcatgt caccgttggc tccgcgcatt gggcaatact ggctgcaact gcctgccaca
     1561 ttcggcccac gggagctgcc tgcgctttgg cattttctcg attctctccc tgccgaattt
     1621 aattatggtg tggaagtccg ccatccacag tttttcgcca aaggggaaga ggaacaaacg
     1681 cttaatcgcg gtttacatca gcgcggcgtt aatcgggtga ttttagacag ccgcccggtt
     1741 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
     1801 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
     1861 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
     1921 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
     1981 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
     2041 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
     2101 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
     2161 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
     2221 tggttttagc gaactgcaaa gcgctattcg cattcatggt aacgcggtgg aatatattcc
     2281 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
     2341 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
     2401 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
     2461 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
     2521 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     2581 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtagctgaa gtcttcccgg
     2641 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     2701 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     2761 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     2821 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     2881 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     2941 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     3001 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     3061 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     3121 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     3181 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     3241 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     3301 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     3361 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     3421 caacgcggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     3481 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     3541 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     3601 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     3661 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     3721 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     3781 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     3841 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     3901 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     3961 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     4021 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     4081 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     4141 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     4201 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     4261 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     4321 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     4381 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     4441 agcagactta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     4501 cagcgcagag ttaccggcac aggcatttgc cagctgcgag ctggggatat ccgccgttaa
     4561 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     4621 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     4681 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     4741 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     4801 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     4861 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     4921 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     4981 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     5041 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     5101 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     5161 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     5221 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     5281 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     5341 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     5401 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     5461 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     5521 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     5581 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     5641 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     5701 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     5761 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     5821 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     5881 ttccgccgcc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     5941 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     6001 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     6061 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     6121 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     6181 gctccaggca atttttaagg tattcagcgt gttcattccc cacagcacca caacctggct
     6241 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     6301 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     6361 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     6421 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     6481 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     6541 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     6601 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     6661 gtttttcgcc agcgcacctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     6721 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     6781 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     6841 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     6901 atctttggcg ttgtactgga aataccgcgt taaaatgtcc agttcgtttt cgctcatgct
     6961 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     7021 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     7081 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     7141 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     7201 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     7261 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     7321 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     7381 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     7441 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     7501 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     7561 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     7621 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     7681 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     7741 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     7801 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     7861 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     7921 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     7981 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     8041 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     8101 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     8161 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     8221 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     8281 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     8341 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     8401 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     8461 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgttcc
     8521 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     8581 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     8641 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     8701 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     8761 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     8821 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cttaattcgc ggaccgtgcg
     8881 cacatcctca agaatggcgg caaactcacc gtcgcagtag caaaaatcac caccgcgtgg
     8941 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     9001 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     9061 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     9121 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     9181 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     9241 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     9301 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     9361 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     9421 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     9481 tgatcatatt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     9541 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     9601 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     9661 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     9721 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     9781 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     9841 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     9901 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     9961 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
    10021 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
    10081 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
    10141 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
    10201 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
    10261 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
    10321 attattggtg acgcagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
    10381 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
    10441 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
    10501 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
    10561 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
    10621 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
    10681 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
    10741 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgagtttaa aaacaaggaa
    10801 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
    10861 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
    10921 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcagcc
    10981 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
    11041 ggcaaaccgt tcaaaacccg cgcgggtggt acagtgaaac tggcagacct gctggatgaa
    11101 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
    11161 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
    11221 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
    11281 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
    11341 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
    11401 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
    11461 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
    11521 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    11581 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    11641 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    11701 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    11761 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    11821 cagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    11881 atacggttat agtaaacgac catgacattt cagccatcaa agattgttgg caaaaaaatt
    11941 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    12001 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    12061 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    12121 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    12181 atttaagtga cccttattct gaatcagaat agctatcatg tatgatcata cagaccaata
    12241 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    12301 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    12361 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    12421 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    12481 aaccgcccac cgcctggcac ttcccagata aatcgcaacg gttctgctgc cgctataccg
    12541 gaaaaggcca cggtggtacc gctctgcccc tctaattcga cacaacgaga ttgcgaacac
    12601 aagcgaaccc gcagtcctgc aggtgtcggg ccgataagct gataacgcca cgccaccagc
    12661 gtcatcaatc ctgaagcagg ttgtcgcgca gaaagaggtg cagacgacat cgactcaccg
    12721 cgatgattta gcgtaattcc gacactactt gcctgccaca tcccctcccc ggcggcttgc
    12781 accagcagcg gaaacaataa tatcgctaat aagattctca tcatttaccg ccaattgtcg
    12841 ccgtcatgcg gatatgtcgg ttatcagaca gttccagatt cgacaggacc actaactgcg
    12901 gcaagctgcg gcgcaggaag cgagacaata atggtcgcag cgcgtggttc accaacaata
    12961 ctggcggcgc acccagcatc tcctgacggg atagcgcttc ctgagtttgc gccagtaaac
    13021 gatccgccag ccctggctcc agtcctcccc cgccctgcag cgcctgtagt aacaaacgtt
    13081 ccagcggtgt atcgaggcca ataacatgga cttcatcttt gccaggaaac cactgctggg
    13141 taatcgcccg tcccaacgcc acgcgcacga cggcggttaa ttcatgtgga tcgctttgga
    13201 tgggcgcatg ttccgccagc gtttcgagaa tggtgcgcat atcgcgaatc ggtacttttt
    13261 catcgaggag attttgcagc actttatgca gtgtggtgag cgtgacgacg ccaggaacga
    13321 gatcttccgt cagctttggc atctcctggg cgacgcgatc caacagctgt tgcgcctcct
    13381 gacgaccaaa cagctctgcg gcatgctggc taatgaggtg gttaagatgc gttgctacca
    13441 ccgtgctggc ctcaaccact gtgtacccct gaatctgcgc ctgttctttt agcgcacttt
    13501 caatccagat agcattcagg ccaaatgccg gatcgacggt cgcctcacca ggtaacgtcc
    13561 cggcagcggt tccagggtta atcgccagcc agcgccccgg ataagcatca ccactgccaa
    13621 tctccacgcc tttcatcaaa atgcgatagc gggcaggttg cagatccata ttgtcgcgaa
    13681 tgtgcaccac tggcggcaga aatcccatct cctgggcaaa tttcttgcgg atactgcgta
    13741 tacggcccaa caactcacca tcctgctgga aattgaccat cgggatcagt cgataaccca
    13801 cttccattcc cagagaatct tccagttgta catcgttcca cgtcgcttcg acaacggtat
    13861 tattctctgc catttttacc ggtttgggtt cggcaggcgc tttttgttcg cgtccgcgta
    13921 tccaccaggc cagccccagc aatccggcag tgaacagcaa aaataccagg ttcggcattc
    13981 caggcaccag gccgagtaaa ccgagcacgg cggcgcttaa caacataacg cttgggttac
    14041 tgaaaagctg attcaccatc tgctcgccaa catcctgatc ggtgctgaca cgcgtaacga
    14101 tgccccccgc ggcggtagaa atcaccagcg ccggaatttg tgccaccaga ccgtcgccaa
    14161 tggtcaatag cgtataactt tccgccgcgt gtcccatgct catgccagtt gcagcacgcc
    14221 gaccagcaac ccgccgacaa cgttaatgac catgatgagg atcccggcaa tggcatcgcc
    14281 gcgaacaaac ttacttgccc cgtccattga gccgtaaaaa tcggcttcct gagtcacttc
    14341 ggagcggcgt tttttcgcct catcttcacc aatcaatcca gcgttaaggt cggcgtcaat
    14401 cgccatctgc ttacccggca taccatcgag aacaaagcgc gcccccactt ctgcgatacg
    14461 cccggcacct ttggtaatga ccataaagtt gatgatcacg agaatgacaa acaccacgat
    14521 accgatagcg aaattgccac caacgaggaa gtgaccgaac gcttcgacca ccttcccagc
    14581 cgccgccgcg ccggtatgcc cttccatcaa aatgatacgg gttgaagcca cgttaagtgc
    14641 cagacgcaac aacgtagtaa acaacagaat ggtcggaaac gcagcaaact caagcgtgcg
    14701 ctgggtaaac atcgccacca gcaacaccat gatcgacaag gcaatattga aggtaaacaa
    14761 caggtcgagt atgaatgcgg gcagtggcag caccatcatc gacaagatca acaggatcaa
    14821 aatcggtccg gcaaggatct gccattatgt cgatttcagg tttgcgggca ggcgcagcat
    14881 cgcggccaga ttactcatgg gtcggtttct cgttaataaa atccagggct tccggcaccg
    14941 gaagatgagt aggtgtacag ggcgctgtcc accagccaga cgccagcgtt tcagttgcca
    15001 gacccaggcc agcacttccg ccaccgcggc gtacagttga cccgggattt gttgaccaat
    15061 ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct tcaagcgtcg gggcgttatt
    15121 ttcagcgcca atttcacgaa tacgcagcgc gaccagccct gcacctttag cgaccacttt
    15181 cggtgcgctc attttgtttt cgtcatactg caacgctacc gaatagtggg tcgggttatt
    15241 gacaatgaca tccgctttcg gcacatcggc catcatccga cgccgtgcgg cagctcgctg
    15301 catctgacag atccgtcctt taacatgggg gtcgccttcg ctttgtttga actcatcacg
    15361 aatatcctgc cgcgacattc gcagcttttt caggtggctg aagatttgga aaaagacgtc
    15421 aaatcccacc attggaatga caccaagcac caccagcagt gcgcatagcc ctaccaaatc
    15481 catcgcatta cccatggcgg taatcggtga ctcggccatt aagcgcatca tctgcggcca
    15541 gtgatgccag agaaaaaacc ccgtcacgct gccaaccagg atggttttca aaattgcctt
    15601 aagcaactcc gcgctagtct gtgccgagaa tatccgttta atgcccggta gcgggttgag
    15661 tttggaaaac ttcggctgca aggatttgcc gctaaatacc agccccccca gcataaccgg
    15721 agaaatgagc gccaccagca ccacgccgct aatcagcggt agcagcgcca gcatggcttc
    15781 tctgatcagc agaataattt gcccgaggat cagattcggg tctttgataa tactgtgatc
    15841 aaaatgcagc ccagcggaga gcatgcccga caatcgacgg gccagcgaca caccgccaaa
    15901 ccagataaca ctaacgccca ccagcaaaat cagcagtgag gtcagttcac gggaacgcgg
    15961 gatttgcccc tcttcccgcg ctttttctag tcggtgaggt gtgggggctt ctgttttgtc
    16021 gtcgctctcg tcagacacgt cgccaatcct ggaaagagta ttaagcgtaa atgatgccag
    16081 agcgcaaagc gttcaatggt ttaagtaagg ggcaaaacag gcgggattta gggcgtttat
    16141 gcaaaaatag gttcagttgc agaacgtttt gcgaaccatt ttccagttct gatatttagc
    16201 catttagcct atagaatcgc cctccgcctc tctgttagtt tgctaataat taaattatta
    16261 caaaggtgat caaatggaat ggcaacaatt gcttaactgc aaccgtcgga aagataatct
    16321 aaaaaaaacc aacatagcat ttttacatcc ccttgctagc ggaagacaag agatcgagcg
    16381 ggattacgac cgattacttt ttgctgcacc aactcgtcgg cttgcggata aaacacaagt
    16441 tttcccgtta gatcaaaatg acagcatacg gacgaggctg actcattctc acgaagtagc
//
LOCUS       DBKEKI010000083        15985 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.083, whole genome shotgun sequence.
ACCESSION   DBKEKI010000083 DBKEKI010000000
VERSION     DBKEKI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.083"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..394)
                     /locus_tag="ACUQPX_002686"
     CDS             complement(<1..394)
                     /locus_tag="ACUQPX_002686"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HHV9057356.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKN"
     gene            773..1084
                     /gene="rpsJ"
                     /locus_tag="ACUQPX_002687"
     CDS             773..1084
                     /gene="rpsJ"
                     /locus_tag="ACUQPX_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HHV9057357.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1117..1746
                     /gene="rplC"
                     /locus_tag="ACUQPX_002688"
     CDS             1117..1746
                     /gene="rplC"
                     /locus_tag="ACUQPX_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HHV9057358.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1757..2362
                     /gene="rplD"
                     /locus_tag="ACUQPX_002689"
     CDS             1757..2362
                     /gene="rplD"
                     /locus_tag="ACUQPX_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HHV9057359.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2359..2661
                     /gene="rplW"
                     /locus_tag="ACUQPX_002690"
     CDS             2359..2661
                     /gene="rplW"
                     /locus_tag="ACUQPX_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HHV9057360.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2679..3500
                     /gene="rplB"
                     /locus_tag="ACUQPX_002691"
     CDS             2679..3500
                     /gene="rplB"
                     /locus_tag="ACUQPX_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HHV9057361.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKLGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3517..3795
                     /gene="rpsS"
                     /locus_tag="ACUQPX_002692"
     CDS             3517..3795
                     /gene="rpsS"
                     /locus_tag="ACUQPX_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HHV9057362.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3810..4142
                     /gene="rplV"
                     /locus_tag="ACUQPX_002693"
     CDS             3810..4142
                     /gene="rplV"
                     /locus_tag="ACUQPX_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HHV9057363.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4160..4861
                     /gene="rpsC"
                     /locus_tag="ACUQPX_002694"
     CDS             4160..4861
                     /gene="rpsC"
                     /locus_tag="ACUQPX_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HHV9057364.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4874..5284
                     /gene="rplP"
                     /locus_tag="ACUQPX_002695"
     CDS             4874..5284
                     /gene="rplP"
                     /locus_tag="ACUQPX_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HHV9057365.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5284..5475
                     /gene="rpmC"
                     /locus_tag="ACUQPX_002696"
     CDS             5284..5475
                     /gene="rpmC"
                     /locus_tag="ACUQPX_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HHV9057366.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5475..5729
                     /gene="rpsQ"
                     /locus_tag="ACUQPX_002697"
     CDS             5475..5729
                     /gene="rpsQ"
                     /locus_tag="ACUQPX_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HHV9057367.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5894..6265
                     /gene="rplN"
                     /locus_tag="ACUQPX_002698"
     CDS             5894..6265
                     /gene="rplN"
                     /locus_tag="ACUQPX_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HHV9057368.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6276..6590
                     /gene="rplX"
                     /locus_tag="ACUQPX_002699"
     CDS             6276..6590
                     /gene="rplX"
                     /locus_tag="ACUQPX_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HHV9057369.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6605..7144
                     /gene="rplE"
                     /locus_tag="ACUQPX_002700"
     CDS             6605..7144
                     /gene="rplE"
                     /locus_tag="ACUQPX_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HHV9057370.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7159..7464
                     /gene="rpsN"
                     /locus_tag="ACUQPX_002701"
     CDS             7159..7464
                     /gene="rpsN"
                     /locus_tag="ACUQPX_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HHV9057371.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7498..7890
                     /gene="rpsH"
                     /locus_tag="ACUQPX_002702"
     CDS             7498..7890
                     /gene="rpsH"
                     /locus_tag="ACUQPX_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HHV9057372.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7903..8436
                     /gene="rplF"
                     /locus_tag="ACUQPX_002703"
     CDS             7903..8436
                     /gene="rplF"
                     /locus_tag="ACUQPX_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HHV9057373.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8446..8799
                     /gene="rplR"
                     /locus_tag="ACUQPX_002704"
     CDS             8446..8799
                     /gene="rplR"
                     /locus_tag="ACUQPX_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HHV9057374.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8814..9317
                     /gene="rpsE"
                     /locus_tag="ACUQPX_002705"
     CDS             8814..9317
                     /gene="rpsE"
                     /locus_tag="ACUQPX_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HHV9057375.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9321..9500
                     /gene="rpmD"
                     /locus_tag="ACUQPX_002706"
     CDS             9321..9500
                     /gene="rpmD"
                     /locus_tag="ACUQPX_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HHV9057376.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9504..9938
                     /gene="rplO"
                     /locus_tag="ACUQPX_002707"
     CDS             9504..9938
                     /gene="rplO"
                     /locus_tag="ACUQPX_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HHV9057377.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9946..11277
                     /gene="secY"
                     /locus_tag="ACUQPX_002708"
     CDS             9946..11277
                     /gene="secY"
                     /locus_tag="ACUQPX_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HHV9057378.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11309..11425
                     /gene="rpmJ"
                     /locus_tag="ACUQPX_002709"
     CDS             11309..11425
                     /gene="rpmJ"
                     /locus_tag="ACUQPX_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HHV9057379.1"
                     /translation="MKVRASVRKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11572..11928
                     /gene="rpsM"
                     /locus_tag="ACUQPX_002710"
     CDS             11572..11928
                     /gene="rpsM"
                     /locus_tag="ACUQPX_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HHV9057380.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11945..12334
                     /gene="rpsK"
                     /locus_tag="ACUQPX_002711"
     CDS             11945..12334
                     /gene="rpsK"
                     /locus_tag="ACUQPX_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HHV9057381.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12368..12988
                     /gene="rpsD"
                     /locus_tag="ACUQPX_002712"
     CDS             12368..12988
                     /gene="rpsD"
                     /locus_tag="ACUQPX_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HHV9057382.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13014..14003
                     /locus_tag="ACUQPX_002713"
     CDS             13014..14003
                     /locus_tag="ACUQPX_002713"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HHV9057383.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14044..14427
                     /gene="rplQ"
                     /locus_tag="ACUQPX_002714"
     CDS             14044..14427
                     /gene="rplQ"
                     /locus_tag="ACUQPX_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HHV9057384.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14534..14902
                     /locus_tag="ACUQPX_002715"
     CDS             14534..14902
                     /locus_tag="ACUQPX_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HHV9057385.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            14913..15338
                     /gene="zntR"
                     /locus_tag="ACUQPX_002716"
     CDS             14913..15338
                     /gene="zntR"
                     /locus_tag="ACUQPX_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HHV9057386.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15394..15612
                     /gene="arfA"
                     /locus_tag="ACUQPX_002717"
     CDS             15394..15612
                     /gene="arfA"
                     /locus_tag="ACUQPX_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HHV9057387.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15609..>15985)
                     /gene="mscL"
                     /locus_tag="ACUQPX_002718"
     CDS             complement(15609..>15985)
                     /gene="mscL"
                     /locus_tag="ACUQPX_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HHV9057388.1"
                     /translation="RGNVVDLAVGVIIGAAFGKIVSSLVADIIMPPLGLLIGGIDFKQ
                     FAVTLRDAQGGIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKLNRKKEEPAAAPA
                     PTKEEVLLAEIRDLLKEQNNRS"
BASE COUNT         4121 a         3762 c         4270 g         3832 t
ORIGIN      
        1 ggttttttaa tgattctttt cctctcatga gcagaccaat aactacggct ccgcaagcga
       61 atgaggcagc aaggaaaacc agtcgtggta agaaggccca tgaatgccag gcgccaaggg
      121 cggcgagaaa cttcacatca ccatagccta atccttcttt atgtcgcagt atgcgatacc
      181 cccagtaaat gacagcgaat gtgccgtaac caacaattgc cccccataac gcatcggcta
      241 aaccatcagg atgacaaact tgatagaaca aaagacctga ccagagtaac gggcacgtaa
      301 atctgtcagg taacaagcca tgctttgcat cccagaaaaa aagtagggct gataaacaag
      361 cgtacaaaat tagaaaggga agggtagtgt ccataaaaca tcctctgtaa ttggagtatc
      421 accatcgaat aactccggat gctcagcaac agagagagcg ctgatttgtt aacctgattc
      481 cggtgaaaaa cgctatcttc acaaccttac aaatttcgtg gaatgcagct cgcaatctgc
      541 gcgttgcgat taatttctac ccgatgcttg cgtcaggctc agatttgcgt ataatgcgcg
      601 ggcttgtcgt agttgacagc aggttcaatc tgaacctcag ttagccgaat ttggctacct
      661 aacaatgctc ccaatcgggg agctacgtaa gaacggttac actctcccat caatcgtaat
      721 gggtctgagg agtaatcatt ttcgtttata aaataattgg agctctggtc tcatgcagaa
      781 ccaaagaatc cgtatccgcc tgaaagcgtt tgatcatcgt ctgatcgatc aagcaaccgc
      841 ggaaatcgtc gagactgcca agcgcactgg tgcgcaggtc cgtggtccga tcccgctgcc
      901 gacacgcaaa gagcgcttca ctgttctgat ctccccgcac gtcaacaaag acgcgcgcga
      961 tcagtacgaa atccgtactc acttgcgtct ggttgacatc gttgagccaa ccgagaaaac
     1021 cgttgatgct ctgatgcgtc tggatctggc tgccggtgta gacgtgcaga tcagcctggg
     1081 ttaatcaggt cattgagcga ttgagaggtt gaaacaatga ttggtttagt cggtaaaaaa
     1141 gtgggtatga cccgtatctt cacagaagac ggcgtttcta tcccagtaac cgtaatcgaa
     1201 gttgaagcaa accgcgttac tcaggttaaa gacttggcta acgatggcta ccgtgctatt
     1261 caggtgacca ccggtgctaa aaaagctaac cgtgtgacca agcctgaagc tggccacttc
     1321 gctaaagctg gcgtagaagc tggccgtggt ctgtgggaat tccgcctggc tgaaggcgaa
     1381 gagttcactg taggtcagag cattagcgtt gaactgtttg ctgacgttaa aaaagttgac
     1441 gtaactggca cctctaaagg taaaggtttc gcaggtaccg ttaagcgctg gaacttccgt
     1501 acccaggacg ctactcacgg taactccttg tctcaccgcg ttccgggttc tatcggtcag
     1561 aaccagactc cgggcaaagt gttcaaaggc aagaaaatgg caggtcagat gggtaacgaa
     1621 cgtgtaaccg ttcagagcct tgacgtagta cgcgttgacg ctgagcgcaa cctgctgctg
     1681 gttaaaggtg ctgtcccggg tgcaaccggt agcgacctga tcgttaaacc agctgtgaag
     1741 gcgtaaggag atagcaatgg aattagtatt gaaagacgcg cagagcgcgc tgactgtttc
     1801 cgaaactacc ttcggtcgtg atttcaacga agcgctggtt caccaggttg ttgttgctta
     1861 tgcagctggt gctcgtcagg gtactcgtgc tcagaagact cgtgctgaag taactggttc
     1921 cggtaaaaaa ccgtggcgcc agaaaggcac cggccgtgcg cgttctggtt ctatcaagag
     1981 cccgatctgg cgttctggtg gcgtgacctt tgctgctcgt ccgcaggacc acagtcaaaa
     2041 agttaacaag aagatgtacc gcggcgcgct gaaaagcatc ctgtccgaac tggtacgtca
     2101 ggatcgtctg atcgttgtcg agaagttctc tgtagaagcg ccgaaaacta agctgctggc
     2161 acagaaactg aaagacatgg ctctggaaga tgtgctgatc atcaccggtg agctggacga
     2221 aaacctgttc ctggctgcgc gcaacctgca caaggttgac gtacgcgatg caactggtat
     2281 cgacccggtt agcctgatcg ccttcgacaa agtcgtaatg actgctgatg ctgttaagca
     2341 agttgaggag atgctggcat gattcgtgaa gaacgtctgc tgaaggtgct gcgtgcaccg
     2401 cacgtttctg aaaaagcgtc tactgcgatg gaaaaatcca acaccatcgt actcaaagtt
     2461 gctaaagacg cgaccaaagc agaaatcaaa gctgctgtgc agaaactgtt tgaagtcgaa
     2521 gtcgaagtcg ttaacaccct ggtagttaaa gggaaagtta aacgtcacgg acagcgtatc
     2581 ggtcgtcgta gcgactggaa aaaagcttac gtcaccctga aagaaggcca gaatctggac
     2641 ttcgttggcg gcgctgagta agtcggagga gtaatacaat ggcagttgtt aaatgtaaac
     2701 cgacatctcc gggtcgtcgc cacgtagtta aagtggttaa ccctgagctg cacaagggca
     2761 aaccttttgc tccgttgctg gaaaaaaaca gcaaatccgg tggtcgtaac aacaatggcc
     2821 gtatcaccac tcgtcatatc ggtggtggcc acaagcaggc ttaccgtatt gttgacttca
     2881 aacgcaacaa agacggtatc ccggcagttg ttgaacgtct tgagtacgat ccgaaccgtt
     2941 ccgcgaacat cgcgctggtt ctgtacaaag acggtgaacg ccgttacatc ctggccccta
     3001 aaggcctgaa agctggcgac cagattcagt ctggcgttga tgctgcaatc aaactaggta
     3061 acaccctgcc gatgcgcaac atcccggttg gttctactgt tcataacgta gaaatgaaac
     3121 caggtaaagg cggtcagctg gcacgttccg ctggtactta cgttcagatc gttgctcgtg
     3181 atggtgctta tgtcaccctg cgtctgcgtt ctggtgaaat gcgtaaagta gaagcagact
     3241 gccgtgcaac tctgggcgaa gttggcaatg ctgagcatat gctgcgcgtt ctgggtaaag
     3301 caggtgctgc acgctggcgt ggtgttcgtc cgaccgttcg cggtaccgcg atgaacccgg
     3361 tagaccaccc acatggtggt ggtgaaggtc gtaactttgg taagcacccg gtaactccgt
     3421 ggggcgttca gaccaaaggt aagaagaccc gcagcaacaa gcgtactgat aaattcatcg
     3481 tacgtcgccg tagcaaataa ttttagagga taagccatgc cacgttctct caagaaaggt
     3541 ccttttattg acctgcactt gctgaagaag gtagagaaag cggtggaaag cggagataag
     3601 aagcccctgc gcacttggtc ccgtcgttca acgatctttc ctaacatgat cggtttgacc
     3661 atcgctgtcc ataatggtcg tcagcacgtt ccggtatttg taaccgacga aatggtcggt
     3721 cacaaactgg gtgaattcgc accgactcgt acttatcgcg gccacgctgc tgataaaaaa
     3781 gcgaagaaga aataaggtag gaggaagaga tggaaactat cgctaaacat cgccatgctc
     3841 gttcttctgc tcagaaggtt cgccttgttg ctgacctgat tcgcggtaag aaagtgtcgc
     3901 aggctctgga tattttgacc tacaccaaca agaaagcggc tgtactagtc aagaaagttc
     3961 tggaatctgc cattgctaac gctgaacaca acgatggcgc tgacattgac gatctgaaag
     4021 ttacgaaaat tttcgtagac gaaggcccga gcatgaagcg cattatgccg cgtgcaaaag
     4081 gtcgtgcaga tcgcatcctg aagcgcacca gccacatcac tgtggttgtg tccgatcgct
     4141 gagactctgg agactagcaa tgggtcagaa agtacatcct aatggtattc gcctgggtat
     4201 tgtaaaacca tggaactcta cctggtttgc gaacaccaaa gaattcgctg acaacctgga
     4261 cagcgatttt aaagtacgtc agtacctgac taaggaactg gctaaagcgt ccgtatctcg
     4321 tatcgttatc gagcgtccgg ctaagagcat ccgtgtaacc attcacactg ctcgcccggg
     4381 tatcgttatc ggtaaaaaag gtgaagacgt agaaaaactg cgtaaggtcg tagcggacat
     4441 cgctggcgtt cctgcacaga tcaacatcgc cgaagttcgt aagcctgaac tggacgcaaa
     4501 actggttgct gacagcatca cttctcagct ggaacgtcgc gttatgttcc gtcgtgctat
     4561 gaagcgtgct gtacagaacg caatgcgtct gggcgctaaa ggtattaaag ttgaagttag
     4621 cggccgtctg ggcggcgcgg aaatcgcacg taccgaatgg taccgcgaag gtcgcgtacc
     4681 gctgcacact ctgcgtgctg acatcgacta caacacctct gaagcgcaca ccacttacgg
     4741 tgtaatcggc gttaaagtgt ggatcttcaa aggcgagatc ctgggtggta tggctgctgt
     4801 tgaacaaccg gaaaaaccgg ctgctcagcc taaaaagcag cagcgtaaag gccgtaaata
     4861 aggagcgtcg ctgatgttac aaccaaagcg tacaaaattc cgtaaaatgc acaaaggccg
     4921 taaccgcggt ctggcgcagg gtacggatgt tagcttcggc agcttcggtc tgaaagctgt
     4981 tggccgtggt cgtctgactg cccgtcagat cgaagcagca cgtcgtgcta tgacccgtgc
     5041 agttaagcgt caaggtaaga tctggatccg tgtgttcccg gacaaaccga tcactgaaaa
     5101 gccgctggca gtgcgtatgg gtaaaggtaa aggtaacgtg gagtattggg ttgccttgat
     5161 tcagccgggt aaagtcctgt atgaaatgga cggtgttccg gaagagctgg cccgtgaagc
     5221 attcaagctg gcagcagcga aactgccgat taaaaccacc tttgtaacta agacggtgat
     5281 gtaatgaaag caaaagagct gcgtgagaag agcattgaag agctgaacac cgagctgctg
     5341 aacctgctgc gtgagcagtt caacctgcgt atgcaggctg caagtggcca gctgcaacag
     5401 tctcacctgt tgaagcaagt gcgtcgcgat gtcgcacgcg ttaagacttt actgaacgag
     5461 aaggcgggtg cgtaatgacc gataaaatcc gtactctgca aggtcgcgtt gttagcgaca
     5521 aaatggagaa atccattgtt gttgctatcg aacgttttgt gaaacacccg atctacggta
     5581 aattcatcaa gcgtacgacc aaactgcacg tacatgacga gaacaacgaa tgcggtatcg
     5641 gtgacgtggt tgaaatccgc gaatgccgtc cgctgtccaa gactaaatcc tggacgctgg
     5701 ttcgcgttgt agagaaagct gttctgtaat acagtacact ctctcgatac gaataaacgg
     5761 ctcagaaatg agccgtttat tttttctacc catatccttg aagcggtgtt ataatgccgc
     5821 gccctcgata tggggatttt taacgacctg atattcgggt ctcagtagta gttgacatta
     5881 gcggagcact aaaatgatcc aagaacagac tatgctgaac gtcgccgaca actccggtgc
     5941 acgtcgcgta atgtgtatca aggttctggg tggctcgcac cgtcgctacg caggcgtagg
     6001 cgacatcatc aagatcacca tcaaagaagc aattccgcgt ggtaaggtca aaaaaggtga
     6061 tgtgctgaag gcggtagtgg tgcgcaccaa gaagggtgtt cgtcgcccgg acggttctgt
     6121 cattcgcttc gatggtaatg cttgtgttct tctgaacaac aacagcgagc agcctatcgg
     6181 tacgcgtatt tttgggccgg taactcgtga gcttcgtagt gagaagttca tgaaaattat
     6241 ctctctggca ccagaagtac tctaaggagc gaatcatggc agcgaaaatc cgtcgtgatg
     6301 acgaagttat cgtgttaacc ggtaaagata aaggtaaacg cggtaaagtt aagaatgtcc
     6361 tgtcttccgg caaggtcatt gttgaaggta tcaacctggt taagaaacat cagaagccgg
     6421 ttccggccct gaaccaaccg ggtggcatcg ttgaaaaaga agccgctatt caggtttcca
     6481 acgtagcaat cttcaatgcg gcaaccggca aggctgaccg tgtaggcttt agattcgaag
     6541 acggcaaaaa agtccgtttc ttcaagtcta acagcgaaac tatcaagtaa tttggagtag
     6601 tacgatggcg aaactgcatg attactacaa agacgaagta gttaaaaaac tcatgactga
     6661 gtttaactac aattctgtca tgcaagtccc tcgggtcgag aagatcaccc tgaacatggg
     6721 tgttggtgaa gcgatcgctg acaaaaaact gctggataac gcagcagcag acctggcagc
     6781 aatctccggt caaaaaccgc tgatcaccaa agcacgcaaa tctgttgcag gcttcaaaat
     6841 ccgtcagggc tatccgatcg gctgtaaagt aactctgcgt ggcgaacgca tgtgggagtt
     6901 ctttgagcgc ctgatcacta ttgctgtacc tcgtatccgt gacttccgtg gcctgtccgc
     6961 taagtctttc gacggtcgtg gtaactacag catgggtgtc cgtgagcaga tcatcttccc
     7021 agaaatcgac tacgataaag tcgaccgcgt tcgtggtttg gatattacca ttaccactac
     7081 tgcgaaatct gacgaagaag gccgcgctct gctggctgcc tttgacttcc cgttccgcaa
     7141 gtaaggtagg gttactaaat ggctaagcaa tcaatgaaag cacgcgaagt aaaacgcgta
     7201 gctttagctg ataaatactt cgcgaaacgc gctgaactga aagcgatcat ctctgatgtg
     7261 aacgcttccg acgaagatcg ttggaacgct gttctcaagc tgcagactct gccgcgtgat
     7321 tccagcccgt ctcgtcagcg taaccgctgc cgtcaaacag gtcgtccgca tggtttcctg
     7381 cggaagttcg ggttgagccg tattaaggtc cgtgaagccg ctatgcgcgg tgaaatcccg
     7441 ggtctgaaaa aggctagctg gtaattgtca ccaattgaat cacgggagta aagacagatg
     7501 agcatgcaag atccgatcgc ggatatgctg acccgtatcc gtaacggtca ggccgcgaac
     7561 aaagctgcgg tcaccatgcc ttcctccaag ctgaaagtgg caatcgccaa cgtgctgaag
     7621 gaagaaggtt ttattgaaga ttttaaagtt gaaggcgaca ccaagcctga actggaactt
     7681 actctgaagt atttccaggg caaagctgtt gtagaaagca ttcagcgtgt cagccgccca
     7741 ggtctgcgca tctataaacg taaagatgag ctgccgaaag ttatggcggg tctgggtatc
     7801 gcagttgttt ctacctctaa aggtgttatg actgatcgtg cagcgcgcca ggctggtctt
     7861 ggtggcgaaa ttatctgcta cgtagcctaa tcggaggaaa aaatgtctcg tgttgctaaa
     7921 gcaccggtcg ttgttcctgc cggcgttgac gtaaaaatca acggtcaggt tattacgatc
     7981 aaaggtaaaa acggcgagct gactcgtact ctcaacgatg ctgttgaagt taaacatgca
     8041 gataataccc tgaccttcgg tccgcgtgat ggttacgcag acggttgggc acaggctggt
     8101 accgcgcgtg ccctgctgaa ctcaatggtt atcggtgtta ccgaaggctt cactaagaag
     8161 ctgcagctgg ttggtgtagg ttaccgtgca gcggttaaag gcaatgtgat taacctgtct
     8221 ctgggtttct ctcatcctgt tgaccatcag ctgcctgcgg gtatcactgc tgaatgtccg
     8281 actcagactg aaatcgtgct aaaaggcgct gataagcagg tgatcggcca ggttgcagcg
     8341 gatctgcgcg cctaccgtcg tcctgagcct tacaaaggca agggtgttcg ttacgccgac
     8401 gaagtcgtgc gtaccaaaga ggctaagaag aagtaaggta acactatgga taagaaatct
     8461 gctcgtatcc gtcgtgcgac ccgcgcacgc cgcaagctcc aggagctggg cgcaactcgc
     8521 ctggtggtac atcgtacccc gcgtcacatt tacgcacagg taattgcacc gaacggttct
     8581 gaagttctgg tagctgcttc tactgtagaa aaagctatcg ctgaacaact gaagtacacc
     8641 ggtaacaaag acgcggctgc agctgtgggt aaagctgtcg ctgaacgcgc tctggaaaaa
     8701 ggcatcaaag atgtatcctt tgaccgttcc gggttccaat atcatggtcg tgtccaggca
     8761 ctggcagatg ctgcccgtga agctggcctt cagttctaag gtagaggtgt aagatggctc
     8821 acatcgaaaa acaagctggc gaactgcagg aaaagctgat cgcggtaaac cgcgtatcta
     8881 aaaccgttaa aggtggtcgt attttctcct tcacagctct gactgtagtt ggcgatggta
     8941 acggtcgcgt tggttttggt tacggtaaag cgcgtgaagt tccagcagcg atccagaaag
     9001 cgatggaaaa agcccgtcgc aatatgatta acgtcgcgct gaataacggc actctacaac
     9061 accctgttaa aggtgttcac acgggttctc gcgtattcat gcagccggct tccgaaggta
     9121 ccggtatcat cgccggtggt gcaatgcgcg ccgttctgga agtcgctggg gttcataacg
     9181 ttctggctaa agcatatggt tccaccaacc cgatcaacgt ggttcgtgca actattgatg
     9241 gactggaaaa tatgaattct ccagaaatgg tcgctgccaa gcgtggtaaa tccgttgaag
     9301 aaattctggg gaaataaacc atggcaaaga ctattaaaat tactcaaacc cgcagtgcaa
     9361 tcggtcgtct gccgaaacac aaggcaacgc tgcttggcct gggtctgcgt cgtattggtc
     9421 acaccgtaga gcgcgaggat actcctgcta ttcgcggtat gatcaacgcg gtttccttca
     9481 tggttaaagt tgaggagtaa gagatgcgtt taaatactct gtctccggcc gaaggctcca
     9541 aaaaggcggg taaacgcctg ggtcgtggta tcggttctgg cctcggtaaa accggtggtc
     9601 gtggtcacaa aggtcagaag tctcgttctg gcggtggcgt acgtcgcggt ttcgagggtg
     9661 gtcagatgcc tctgtaccgt cgtctgccga aattcggctt cacttctcgt aaagcagcga
     9721 ttacagccga agttcgtctg tctgacctgg ctaaagtaga aggcggtgtt gtagacctga
     9781 acacgctgaa agcggctaac attatcggta tccagatcga gttcgcgaaa gtgatcctgg
     9841 ctggcgaagt aacgactccg gtaactgttc gtggcctgcg tgttactaaa ggcgctcgtg
     9901 ctgctatcga agctgctggc ggtaaaatcg aggaataagt agcagatggc taaacaaccg
     9961 ggattagatt ttcaaagtgc caaaggtggc ttaggcgagc tgaaacgcag actgctgttt
    10021 gttatcggtg cgctgattgt gttccgtatt ggctctttta ttccgatccc tggtattgat
    10081 gccgctgtac ttgccaaact gcttgagcaa cagcgaggca ccatcattga gatgtttaac
    10141 atgttctctg gtggtgctct cagccgtgct tctatctttg ctctggggat catgccgtat
    10201 atttcggcgt cgatcattat ccagctgctg acggtggttc acccaacgtt ggcagaaatt
    10261 aagaaagaag gggagtctgg tcgtcgtaag atcagccagt acacccgtta cggtactctg
    10321 gtgctggcaa tattccagtc gatcggtatt gctaccggtc tgccgaatat gcctggtatg
    10381 caaggcctgg tgattaaccc gggctttgca ttctacttca ccgctgttgt aagtctggtc
    10441 acaggaacga tgttcctgat gtggttgggc gaacagatca ctgaacgagg tatcggcaac
    10501 ggtatttcaa tcattatctt cgccggtatt gtcgcgggac tcccgccagc cattgcccat
    10561 actatcgagc aagcgcgtca aggcgacctg cacttcctcg tgttgctgtt ggttgcagta
    10621 ttagtatttg cagtgacgtt ctttgttgtg tttgttgagc gtggtcaacg ccgcattgtg
    10681 gtaaactacg cgaaacgtca gcaaggtcgt cgtgtctatg ctgcacagag cacacattta
    10741 ccgctgaaag tgaatatggc gggggtaatc ccggcaatct tcgcttccag tattattctg
    10801 ttcccggcga ccatcgcgtc atggttcggg ggcggtactg gttggaactg gctgacaaca
    10861 atttcgctgt atttgcagcc tgggcaaccg ctttatgtgt tactctatgc gtctgcaatc
    10921 atcttcttct gtttcttcta cacggcgttg gttttcaacc cgcgtgaaac agcagataac
    10981 ctgaagaagt ccggtgcatt tgtaccagga attcgtccgg gagagcaaac ggcgaagtat
    11041 atcgataaag taatgacccg cctgaccctg gttggtgcgc tgtatattac ctttatctgc
    11101 ctgatcccgg agttcatgcg tgatgcaatg aaagtaccgt tctacttcgg tgggacctca
    11161 ctgcttatcg ttgttgtcgt gattatggac tttatggctc aagtgcaaac tctgatgatg
    11221 tccagtcagt atgagtctgc attgaagaag gcgaacctga aaggctacgg ccgataattg
    11281 gtcgcccgag aagttacgga gagtaaaaat gaaagttcgt gcttccgtca ggaaattatg
    11341 ccgtaactgc aaaatcgtta agcgtgatgg tgtcatccgt gtgatttgca gtgccgagcc
    11401 gaagcataaa cagcgccaag gctgattttt tcgcatattt ttcttgcaaa gttgggttga
    11461 gctggctaga ttagccagcc aatcttttgt atgtctgtgc gtttccattt gagtatcctg
    11521 aaaacgggct tttcagcatg gaacgtacat attaaatagt aggagtgcat agtggcccgt
    11581 atagcaggca ttaacattcc tgatcataag catgccgtaa tcgcattaac ttcgatttat
    11641 ggcgtcggca agacccgttc taaagccatc ctggctgcag cgggtatcgc tgaagatgtt
    11701 aagatcagtg agctgtctga aggacaaatc gacacgctgc gtgacgaagt tgccaaattt
    11761 gtcgttgaag gtgatctgcg ccgtgaaatc agcatgagca tcaagcgcct gatggatctt
    11821 ggttgctatc gcggtttgcg tcatcgtcgt ggtctcccgg ttcgcggtca gcgtaccaag
    11881 accaacgcac gtacccgtaa gggtccgcgc aaaccgatca agaaataatc ggggtgattg
    11941 aataatggca aaggcaccaa ttcgtgcacg taaacgtgta agaaaacaag tctctgacgg
    12001 cgtggctcat atccatgctt ctttcaacaa caccatcgtg actatcactg atcgtcaggg
    12061 taacgcgttg ggttgggcaa cagccggtgg ttccggtttc cgtggttctc gcaaatccac
    12121 tccgtttgca gctcaggttg cagcagagcg ttgcgctgac gccgtgaaag aatacggcat
    12181 caagaatctg gaagttatgg ttaaaggtcc gggtccaggc cgcgaatcta ctattcgtgc
    12241 tctgaacgcc gcaggtttcc gcatcactaa cattactgat gtgactccga tccctcataa
    12301 cggttgtcgt ccgccgaaaa aacgtcgcgt ataacgcctc gttttccagg tttgttggag
    12361 aaagaaaatg gcaagatatt tgggtcctaa gctcaagctg agccgtcgtg agggcaccga
    12421 cttattcctt aagtctggcg ttcgcgcgat cgataccaag tgtaaaattg aacaagctcc
    12481 tggccagcac ggtgcgcgta aaccgcgtct gtctgactat ggtgtgcagt tgcgtgaaaa
    12541 gcaaaaagtt cgccgtatct atggtgtgct ggagcgtcag ttccgtaact actacaaaga
    12601 agcagcacgt ctgaaaggca acaccggtga aaacctgttg gctctgctgg aaggtcgtct
    12661 ggacaacgtt gtataccgta tgggcttcgg tgccactcgt gcagaagcac gtcagctggt
    12721 tagccataaa gcaattatgg taaacggtcg tgttgttaac atcgcttctt atcaggttag
    12781 tccgaatgac gttgtaagca ttcgtgagaa agcgaagaag cagtctcgcg tgaaagccgc
    12841 tctggagctg gctgagcagc gtgaaaagcc aacttggctg gaagttgatg ctggcaagat
    12901 ggaaggtacg tttaagcgta agccggagcg ttctgatctg tctgcggaca ttaacgaaca
    12961 cctgatcgtc gagctttact ccaagtaaag cttagtacca aagagaggac acaatgcagg
    13021 gttctgtgac agagtttcta aaaccgcgcc tggttgatat cgagcaagtg agttcgacgc
    13081 acgccaaggt gacccttgag cctttagagc gtggctttgg ccatactctg ggtaacgcac
    13141 tgcgccgtat tctgctctca tcgatgccgg gttgcgcggt gaccgaggtt gagattgatg
    13201 gtgtactaca tgagtacagc accaaagaag gcgttcagga agatatcctg gaaatcctgc
    13261 tcaacctgaa agggctggcg gtgagagttc agggcaaaga tgaagttatt cttaccttga
    13321 ataaatctgg cattggccct gtgactgcag ccgatatcac ccacgacggt gatgtcgaaa
    13381 tcgtcaagcc gcagcacgtg atctgccacc tgaccgatga gaacgcgtct attagcatgc
    13441 gtatcaaagt tcagcgcggt cgtggttatg tgccggcttc tacccgaatt cattcggaag
    13501 aagatgagcg cccaatcggc cgtctgctgg tcgacgcatg ctacagccct gtggagcgta
    13561 ttgcctacaa tgttgaagca gcgcgtgtag aacagcgtac cgacctagac aagctggtca
    13621 tcgaaatgga aaccaacggc acaatcgatc ctgaagaggc gattcgtcgt gcggcaacca
    13681 ttctggctga acaactggaa gctttcgttg acttacgtga tgtacgtcag cctgaagtga
    13741 aagaagagaa accagagttc gatccgatcc tgctgcgccc tgttgacgat ctggaattga
    13801 ctgtccgctc tgctaactgc cttaaagcag aagctatcca ctatatcggt gatctggtac
    13861 agcgtaccga ggttgagctc cttaaaacgc ctaaccttgg taaaaaatct cttactgaga
    13921 ttaaagacgt gctggcttcc cgtggactgt ctctgggcat gcgcctggaa aactggccac
    13981 cggcaagcat cgctgacgag taaccggatc acaggttaag gttttactga gaaggataag
    14041 gtcatgcgcc atcgtaagag tggtcgtcaa ctgaaccgca acagcagcca tcgccaggct
    14101 atgttccgca atatggcagg ttcactggtt cgtcatgaaa tcatcaagac gactctgcct
    14161 aaagcgaaag agctgcgccg cgtagttgag ccgctgatta ctcttgccaa gactgatagc
    14221 gttgctaatc gtcgtctggc attcgcccgt actcgtgata acgagatcgt ggcaaaactg
    14281 tttaacgaac tgggcccgcg tttcgcgagc cgtgccggtg gttacactcg tattctgaag
    14341 tgtggcttcc gtgcaggcga caacgcgccg atggcttaca tcgagctggt tgatcgttca
    14401 gagaaagcag aagctgctgc agagtaatct gaagcaacgt aaaaaaaccc gccccggcgg
    14461 gtttttttat acccgtagta tccccactta tctacaatag ctgtactctt tttgttcatc
    14521 ccctggagta tttatgtggt tacttgacca gtgggcagag cgccatatag cagaagcgca
    14581 agcgaaaggt gagtttgata acctggcagg tagcggcgaa ccattgatac tggatgatga
    14641 ttctcacgtg ccaccggaat tacgtgcggg gtatcgcttg ctgaagaatg ccggttgctt
    14701 accgccagaa cttgagcaac ggagagaagc aattcagctt ctggatattc tcaaaggtat
    14761 ccgtcacgat gatccgcaat atcaagaggt tagccgtcga ttgtcattac tggaattgaa
    14821 gctgcgacaa gctggattga gtaccgattt tttacgcggc gattatgctg acaagttgtt
    14881 ggacaaaatc aacgataact agtggagtat gtatgtatcg cattggtgag ctggcaaaaa
    14941 tggcggaagt aacacctgac acgattcgtt attacgaaaa acagcagatg atggagcatg
    15001 aagtgcgtac tgaaggcggg tttcgcctgt ataccgaaag cgatctccag cgattgaaat
    15061 ttatccgcca tgccagacaa ctaggtttca gtctggagtc gatccgcgag ttgctgtcga
    15121 tccgcatcga tcctgaacac catacctgtc aggagtcaaa aggcattgtg caggaaagat
    15181 tgcaggaagt cgaagcacgg atagccgagt tgcagagtat gcagcgttcc ttgcaacgcc
    15241 ttaacgatgc ctgttgtggg accgcccata gcagtgttta ttgctcgatt cttgaagctc
    15301 ttgaacaagg ggcgagtggc gttaagagtg gctgttgatt ttttgcactg acaggattac
    15361 actcgcgccg ttaaataacc aactggagtt tttatgagcc gatatcagca tactaaaggg
    15421 cagataaagg ataatgcgat agaagcatta ctacatgatc ccttattccg acagcgagta
    15481 gagaaaaata agaaagggaa aggcagttac atgcgaaaag gaaaacatgg caatcggggg
    15541 aactgggagg ccagtggcaa gaaagtaaat cactttttta ccactggtct tctgctttca
    15601 ggcgcttgtt aagagcggtt attctgctct ttcagcaaat cacgaatttc tgccagtaat
    15661 acttcttctt tagttggtgc aggtgcggca gctggttctt cttttttccg attcagtttg
    15721 ttgattagct taatcgccat aaagatggca aaggccacaa tcagaaaatc aaagacgttt
    15781 tgaatgaaga caccgtaatg catcacaaca gcagggatac ccccctgcgc atcgcgtagc
    15841 gtgacagcaa actgtttaaa gtcgatcccg ccaattaata agcccagagg tggcatgatg
    15901 atatcggcaa ccagtgaaga tacaatcttc ccgaatgccg caccgataat gacacccacc
    15961 gccaaatcca ccacgttccc gcgca
//
LOCUS       DBKEKI010000084        15924 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.084, whole genome shotgun sequence.
ACCESSION   DBKEKI010000084 DBKEKI010000000
VERSION     DBKEKI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.084"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..178
                     /locus_tag="ACUQPX_002719"
     CDS             <1..178
                     /locus_tag="ACUQPX_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HHV9057389.1"
                     /translation="DEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDL
                     PRNCSGKIIRKNLK"
     gene            287..1072
                     /gene="caiD"
                     /locus_tag="ACUQPX_002720"
     CDS             287..1072
                     /gene="caiD"
                     /locus_tag="ACUQPX_002720"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HHV9057390.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            1078..1668
                     /gene="caiE"
                     /locus_tag="ACUQPX_002721"
     CDS             1078..1668
                     /gene="caiE"
                     /locus_tag="ACUQPX_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HHV9057391.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1754..2149)
                     /gene="caiF"
                     /locus_tag="ACUQPX_002722"
     CDS             complement(1754..2149)
                     /gene="caiF"
                     /locus_tag="ACUQPX_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HHV9057392.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(2411..5632)
                     /gene="carB"
                     /locus_tag="ACUQPX_002723"
     CDS             complement(2411..5632)
                     /gene="carB"
                     /locus_tag="ACUQPX_002723"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HHV9057393.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(5650..6798)
                     /gene="carA"
                     /locus_tag="ACUQPX_002724"
     CDS             complement(5650..6798)
                     /gene="carA"
                     /locus_tag="ACUQPX_002724"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HHV9057394.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFLGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(7254..8075)
                     /gene="dapB"
                     /locus_tag="ACUQPX_002725"
     CDS             complement(7254..8075)
                     /gene="dapB"
                     /locus_tag="ACUQPX_002725"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HHV9057395.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(8242..9156)
                     /gene="rihC"
                     /locus_tag="ACUQPX_002726"
     CDS             complement(8242..9156)
                     /gene="rihC"
                     /locus_tag="ACUQPX_002726"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HHV9057396.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(9222..10172)
                     /gene="ispH"
                     /locus_tag="ACUQPX_002727"
                     /gene_synonym="lytB"
     CDS             complement(9222..10172)
                     /gene="ispH"
                     /locus_tag="ACUQPX_002727"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HHV9057397.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(10174..10623)
                     /gene="fkpB"
                     /locus_tag="ACUQPX_002728"
     CDS             complement(10174..10623)
                     /gene="fkpB"
                     /locus_tag="ACUQPX_002728"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HHV9057398.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(10710..11204)
                     /gene="lspA"
                     /locus_tag="ACUQPX_002729"
     CDS             complement(10710..11204)
                     /gene="lspA"
                     /locus_tag="ACUQPX_002729"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HHV9057399.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(11204..14020)
                     /gene="ileS"
                     /locus_tag="ACUQPX_002730"
     CDS             complement(11204..14020)
                     /gene="ileS"
                     /locus_tag="ACUQPX_002730"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HHV9057400.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYVEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(14063..15004)
                     /gene="ribF"
                     /locus_tag="ACUQPX_002731"
     CDS             complement(14063..15004)
                     /gene="ribF"
                     /locus_tag="ACUQPX_002731"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HHV9057401.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(15012..15230)
                     /locus_tag="ACUQPX_002732"
     CDS             complement(15012..15230)
                     /locus_tag="ACUQPX_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HHV9057402.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            15333..15596
                     /gene="rpsT"
                     /locus_tag="ACUQPX_002733"
     CDS             15333..15596
                     /gene="rpsT"
                     /locus_tag="ACUQPX_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HHV9057403.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3812 a         4350 c         4019 g         3743 t
ORIGIN      
        1 cgatgaagcc atcaaagcat ttgtggtgct gaatgaaggt gaaacattga gcgaagagga
       61 attttttcgc ttctgcgaac aaaatatggc gaaatttaaa gtgccctctt atctggagat
      121 cagaaaagat ctgccacgta attgctcggg gaaaataatt agaaagaatc tgaaataaat
      181 gaaacggcag ggaactaccc tgccagctaa taatcacgcc ctaaaacaat atgcattttt
      241 tgcagggatg ttgtcgtccc taaaaaagca aaaatggaga aaaggaatga gtgaatcatt
      301 acatctgacc cgcaatggat caattctgga aattaccctt gatcgcccaa aagcgaatgc
      361 tattgatgca aaaaccagct ttgaaatggg cgaagtattt ctaaatttcc gtgacgatcc
      421 gcaattacgt gtcgccatta ttaccggtgc cggagagaaa ttcttttccg cgggctggga
      481 tttaaaagcg gcagcagaag gcgaagcacc ggatgctgac tttggcccgg gtggttttgc
      541 gggattaacc gaaattttca atctcgacaa accagttatc gcagctgtga acggctacgc
      601 ctttggcggc ggtttcgaac tggcgctggc ggcagatttt attgtttgtg ccgataacgc
      661 cagcttcgcc ctgccggaag ccaaactggg catcgttcct gacagcggcg gtgtgctgcg
      721 tctgccgaag atcctgccgc ctaccatcgt caatgaaatg gtgatgaccg gcagacgaat
      781 gggcgcagaa gaggcactgc gttgggggat agtcaaccgc gtggttagcc aggcggaact
      841 gatggataac gcccgcgaac tggctcagca gctggttaac agcgccccgc tggcgattgc
      901 ggcgctgaaa gagatctacc gcaccaccag cgaaatgccg gtagaagaag cgtatcgcta
      961 tattcgcagc ggcgtgttga aacactatcc atcggttctg cattcggaag atgccattga
     1021 agggccgctg gcgtttgccg agaagcgcga tccggtgtgg aaaggacgtt aacaaccgtg
     1081 agctattacg cctttgaggg gttaattccg gtggttcacc cgacggcgtt tgtccatccc
     1141 agtgccgtct tgattggcga tgtgattgtg ggagccggtg tctacatcgg cccactcgcc
     1201 tcactgcgtg gtgactacgg gcggttgatc gtgcaagcgg gagccaatat tcaggatggc
     1261 tgcattatgc atggctactg cgacactgac accatcgttg gggaaaacgg ccatatcggg
     1321 catggagcga tcctgcatgg ttgtgtgatt ggtcgcgatg cattggtcgg gatgaacagc
     1381 gtaattatgg atggcgcggt cattggcgaa gagagcattg ttgccgcaat gagctttgtc
     1441 aaagcaggct ttagtggcga gaaacgccag ttgttgatgg gtacgccagc ccgcgctgta
     1501 cgcagtgtta gtgacgacga gttacactgg aaacggttga ataccaaaga gtatcaggat
     1561 cttgtcgggc gctgtcatgc atcgttacat gaaacgcagc cactgaggca aatggaggaa
     1621 aatcgccccc gtttgcaggg gacgacggat gtgacgccta aacggtgatc ttgccggatg
     1681 cgacctggtt gctggataag atgctacaac atcgtatctg gcaagcagga cgcccggtat
     1741 caacgagata aaattaacga cgcatactct ttgacaacat catctgccac ttctgctcgc
     1801 ggttaagttc cgtgagcgga acggcaggaa tacgcggtgt ctttcttacg ccgactaatt
     1861 tttcccgact gttattgcgc aggcgcgcgt aaatttgctc atcgatatcg accactttaa
     1921 ccagtcgctg acactggcat ccccgccctt ccagcttatt agggatcatc ttaacttcgc
     1981 agcttatttc tgtgacttcc gacagaatat aagtcagggt attaaccgcc ttgctgtgtt
     2041 caatatcgaa atgaatagag atctctcttg ctatcaccca ccgattttca gccatcatcc
     2101 attcggcgat taacaagtag agtggttttt caacatatcc ttcacacata cgaactccag
     2161 tttcacaaat taaaagtctg gctatgactc gttttgaagc aattaaacaa ggccatatta
     2221 aaattgtgga tatatcgata gtatgcaact tcaccgtcga taagtgtgag ctaaatcctg
     2281 cttatgtcat caaaaatatc gattccccaa aagatttact taatgggcaa aaaaaaacgg
     2341 tcctcatcag agaaccgtat tagttattcg atcaaattag cggatgaaaa atatccgcca
     2401 cgacacgcta ttatttgatc tgtgcgtgca tttcctgcac cgaaattact ttttcagtcg
     2461 catcggcatt cagcgccatc gcggtagcga aaccaccgtt cagggtggtg tcataatgca
     2521 ctttatattg cagcgcactg cgacggatca cgcgggagtc ttcaatcgca cgacggcctg
     2581 aggtggtgtt gatgatgtag gtatattcgc cattcttgat acggtcctga atgtgcggac
     2641 ggccttcatg caccttgttt accagacgcg gattgatacc cgcttcgccc agcacaatcg
     2701 ccgtgccgtg ggtcgcatcc agctcgaagc cctgtttcag cagttttgcc gccaggtcca
     2761 ccacgcgttc tttatcgcct tcgcgcacgg aaagcagcgc acgaccgtgt ttcttcatgg
     2821 tggagttgct gcccagctgc gctttggcga acgcttcagc gaaggtgcgg cccacgccca
     2881 tgacttcccc ggtagagcgc atttctggcc ctaacagcgg gtcaacgccc gggaatttat
     2941 tgaacggcag caccacttct ttcaccgagt agtacggcgg aataacttct ttggttacgc
     3001 cctgctcagc cagcgatttg ccagccatca cacgcgccgc cacttttgcc agcggtacgc
     3061 cggtggcttt ggagacgaac ggaacagtac gcgccgcacg cgggtttact tcaatcaggt
     3121 aaacttcgtt gtttttcacc gcaaactgca cgttcatcag accgcgcacc tgcaattcga
     3181 aagccagttt ctgcacctgc tggcgcatca catcctgaat ttcctgactt aaggtgtacg
     3241 ctggcagcga acatgcggag tcaccggagt gcacgcccgc ctgctcgatg tgctccatga
     3301 tgccgccaat cagcaccatt tcgccgtcgc agatggcatc cacgtcaact tctactgcgt
     3361 catcaaggaa atgatccagc aacactggcg catcgttaga cacgctaacc gccgtctgga
     3421 agtagcgacg caggtcagct tcgtcataga cgatttccat cgcccgaccg ccgagaacgt
     3481 aagacggacg caccaccagc gggtagccaa tctcttttgc cttctcaact gccatttcaa
     3541 tagcagtaac ggtggcgttc gccggttgtt tcagtttcag acggtcaacc gcatgctgga
     3601 agcgttcacg gtcttccgca cggtcgatag catccgggct ggtgccgata accggtacgc
     3661 cagcggcttc cagtgcacgc gccagtttca gcggggtctg accgccgtac tgaacgataa
     3721 cgcctttcgg cttctcaata cgcacgattt ccagcacatc ttccagagtt accggctcga
     3781 agtagaggcg atcggaagtg tcgtagtcgg tagagacggt ttccgggtta cagttaacca
     3841 taatggtttc gtaaccgtct tcacgcagcg ccagcgaggc gtgtacgcag cagtagtcga
     3901 attcgatacc ctgaccgata cggtttggac cgccgccaag caccatgatt ttttcacggt
     3961 cggtagacgg attcgcttcg cactcttctt cataagtgga gtacatgtaa gcggtgtcgg
     4021 tggcaaactc tgccgcacag gtatccacgc gcttgtagac cgggtgcagg tcatattggt
     4081 cacgcagctt acggatttcc gcttcgcgta cgcccgccag ttttgccaag cgcgcatcgg
     4141 caaagccttt gcgtttcagc tggcgcagga attcagcgtt caggccagtg atgcccactt
     4201 ccgccacttt ctcttccaga cgcaccagtt cttcaatctg taccaggaac cagcggtcaa
     4261 tgttagtcag gttgaagacg ccgtccacag acaggcccgc gcggaaagca tcggcgatgt
     4321 accagatacg ctctgcgcct gcgtctttca gttcgcgacg gattttggtt aacgcttccg
     4381 ggtcatctag gctcactttc gggtcgaagc cagttgcgcc cacttccagg ccgcgcagcg
     4441 ctttttgcag ggattcctgc tgcgtgcgac caatcgccat cacttcgcca accgatttca
     4501 tctgagtggt cagacggtcg ttagcaccgg cgaatttttc gaagttgaag cgaggaattt
     4561 tagtaaccac atagtcgatg gacggctcga aggaggccgg agtacgtccg ccagtgatgt
     4621 cgttcatcag ttcgtcgagg gtgtaaccca ccgccagttt cgccgccact ttagcaatcg
     4681 ggaaaccggt cgctttcgac gccagcgccg aagaacggga cacgcgtggg ttcatttcga
     4741 taacaatcag gcgaccgttt ttcgggttca ccgcaaactg gacattggaa ccaccggttt
     4801 caacgccgat ttcacgcagc accgccatcg aggcgttacg catgatttga tattctttgt
     4861 cggtcagcgt ttgggctggc gcgacagtga tggagtcacc ggtgtggatg cccatcgcat
     4921 caaagttttc gatagagcag acgatgatgc agttgtcgtt tttatcacgc accacttcca
     4981 tctcgtactc tttccagccg atcagcgact catcaatcag caactctttg gtcggagaga
     5041 gatccagacc gcgggcgcaa atttcttcaa actcttcacg gttataagcg ataccgccgc
     5101 cgctaccgcc catggtaaag gatgggcgaa taatgcacgg gaagcccacg tcagcggcaa
     5161 cagccagcgc ttcttccatc gtgtgtgcga taccggaacg cgcggtttcc agaccaattt
     5221 tcttcatcgc tacgtcgaaa cgacggcggt cttctgcttt atcaatcgca tcggcagtgg
     5281 caccaatcat ggtgacaccg aactcttcca acacgccctg acgttccagc tccagcgcgc
     5341 agttcagcgc cgtctgaccg cccatggttg gcagcaccgc gtccgggcgc tctttttcaa
     5401 taatcttgcg taccacttcc cagtgaatcg gctcgatgta ggtcgcatcg gccatttccg
     5461 ggtcggtcat gatggtcgcc gggttggagt tcaccagaat gacgcggtaa ccctcttcgc
     5521 gcagggcttt acatgcttgc gcgccagagt agtcaaactc acacgcctga ccgataacaa
     5581 tcgggcccgc acccagaatc aggatacttt ttatatctgt acgttttggc atggctcttt
     5641 tactcctgat tacttagcgg ttttacggta ctgctcaatt aacttgataa agtggtcgaa
     5701 caacggcgcg gcgtcgtgtg gaccagggct ggcttcaggg tgaccctgga agctgaacgc
     5761 cggtttatcg atgcgatgaa tgccctgtaa cgtaccgtcg aacagggatt tatgcgtgac
     5821 acgcaggttt gcaggtaatg ttgcttcatc caccgcaaaa ccgtggttct gggcggtgat
     5881 catcaccacg tttttctcca catctttaac cggatggttg ccgccgtggt gaccaaattt
     5941 cattttgaca gtcttcgcac cgctcgccag cgccagcagc tgatgaccga gacagatgcc
     6001 gaataccgga atatcggttt cgaggaactt ctggatggcg gtaatggcgt aatcgcacgg
     6061 ggccgggtcg ccaggaccgt tggagaggaa gatgccgtct ggattcattt tcagcacatc
     6121 ttccgcagaa gtttgcgccg gaacgatggt caggcgacag cctctatcca ccagcatccg
     6181 caggatgttg cgcttggcac caaaatcata agccacgacg tggaacggca gctcgtcttc
     6241 ttttttcgct tctggcaggc cgccggtcaa cgtccagctc ccttgtgtcc agctataggc
     6301 ttctgcggtg gtcacttctt ttgccagatc catgccgttc agacctagga acgcgcgggc
     6361 tttttctaac gccagcgccg catccgggtt atcgcctgcg ataatgcagc cattctgtgc
     6421 gcctttctcg cgcagtaaac gcgtcagctt acgggtatcg atatcggcaa tcgccacgat
     6481 gttatggcgc ttcaggtaag aagagaggtc ttcggtatta cggaagttgc tggcaatcag
     6541 cggcaggtcg cgaatcacca gaccttgtgc atgtacctga gaagattctt catcggcgtc
     6601 attagtgccg acattgccaa tatggggata agtaagagta acgatttgac gagaatagga
     6661 aggatcagtg aggatttctt gataaccggt cattgaagta ttgaaaacga cttccccaac
     6721 cgccgaacct gttgccccta tggcccgacc gtgaaactgg gttccgtctt ccagaaccaa
     6781 tagcgctgac ttaatcaaaa caccctccag agaatattca ctcactttat ttgcatatta
     6841 attcacagtg atgacgtaaa tcaatgcaaa tctgcttacc gacgaatttc tggcaaacgg
     6901 cggcattctg gagatatggg cgctaaaagt caacttaatg gcaggatttt ttatattatt
     6961 ttgtggctca tgcatgtttt acaggcatta agcgacaaaa acatcatcta acctgttctg
     7021 gaaaacgctt gcgcagcaag aagattgcat gaaataaaaa cgagcaggaa aaagtggacc
     7081 aaatggtcaa aatttacatt aaaacaacaa aaatagaaga cgaaacatgt aatttttggt
     7141 agttatagaa aatttaaaag cataacacca aaaataaaag ggctaaaaat taaagccctt
     7201 tataattaat aaattaaatg tgttactttt gcatcataat aaacattttg tggttacaaa
     7261 ttattgagat caagcacatc tcgcatatca aaaagaccac tttccttacc actcaaccac
     7321 aaagccgatc ttaccgcacc gttagcaaat gtcatgcggc tggacgcctt atgggtgatc
     7381 tccagacgct cgccaatatc ggcaaacatc gcggtatgtt cgccaacgat gtcacctgca
     7441 cgcacggtgg caaaaccaat ggtaccaggc acacgttcac cggtatggcc ttcacgactg
     7501 tagaccgcgc aatctttcag atctttatca agggcgtggg cgatcgcctc tcccattgcc
     7561 agtgcggtgc ctgacggcgc atcaacttta tgtctatgat gtgcttcaat aatttcgata
     7621 tcggtgtagt cacccatcac tttggctgct ttctccagca gcttaagcat gacgttaacg
     7681 ccaacgctaa aattggcggc aaagacaatc gcaatatcgg cggcggcgtc acgaattgcc
     7741 tgtttaccgg cttcgtcaaa ccccgtagtg ccgatcacca tccctttgcc atgctgccga
     7801 caaaaagcga gatggttcag cgtaccttcc ggacgggtaa aatcgataaa cacatcaaaa
     7861 tcatctttta tcgcatcaag actgctttgc acggtaacgc ctgttttccc tgcaccagcc
     7921 agctcaccgg cgtcgctgcc cagtaaagaa gatccttcgc gctccagcgc cgcgcccagc
     7981 tgcacgcctt ctaatgccag tgccgcctga atcaactggc ggcccatacg ccccccggct
     8041 cccgcgatgg caacgcggat gtttgcatca tgcatagcta ttctcttttg ttaatttgca
     8101 tagaccgttt tcagagtaac cagcgcatcg ctacgccgcc agccaaaaac gctgataatt
     8161 agaaattaat gataaacagg gagggatatc agtgaaaggc atgagtcgat gaatgactgc
     8221 atgccgataa catgtgacag gttacggcac cagagccagc acctcagcca cccactgttg
     8281 gaagcctttt acattcagat ccaatgccac ctgtacattg gctggtttgc ccaggcaacc
     8341 gtcgatatca accaccgtcg tgcctgaggt aaattcgcct tgagtttcta ccgcgacaaa
     8401 acacggcttg agggtgaata agtccgggcg caccagccag gcgatggcgc agagatcgtg
     8461 cattcgcaga ccgctttgca tactgccgct acggtagtgg ctaaacaggg cgtggagcat
     8521 ttttccggtg cgattcaact ccggtaaagt agcgagatag tcaggggtta atattgcctg
     8581 attggtgaca tccagaccgc acatgacaat ttcgatgccg ctacggaaga cacaggcagc
     8641 cgcttctgga tcggcagcga tattaaactc ggcatttggt gtacagttgc cgcgaccagc
     8701 agaaccgccc atgatcacca gacgacgaat atacggttta cattccgggc attgtaaaag
     8761 taacagcgca atattggtta acgggccgat ggccaccagg gtaacgggct ctggtgcacg
     8821 catcagggca tcccgaatcg ccagaaacgc cggtatcccg agtggctttc ggttgtgctc
     8881 aacaaagtcg tagccagcca ttcccgattc gccgtgcaca gatgctgcat cacgcggtgc
     8941 gcgtaccagc ggcacagcgg ctccctgggc gagcggaatc tccgcattcc agaaatgcag
     9001 cagttgcagg gcattgcggg tagttttctc aaccgagaca ttacccgcga cggtggtcat
     9061 cagttgcagg tcgagttcgg gggcaaaaat cgcggcggca atggcgacgg catcgtcaat
     9121 gccggggtca gtatcgagga agataggtaa acgcatgttt tcttcataaa aaatgccggt
     9181 aacaagaccg gcattttcgc ataacttagg ctgctaatga cttaatcgac ttcacgaata
     9241 tcgacacgca gctctttcgg cacttcgaaa acaatatttt cttcacggcc ttccagcgga
     9301 atggcttcac caccacccaa ctgctgcaaa cgtgccacca cattctgcac cagaatatcc
     9361 ggagccgatg cgcccgcagt cacgccgacg catttaacct ctttcaccca ctcttcctga
     9421 atatctttcg catcgtcaat caaaaacgcg tgtttgccca tacgctgggc cagctccgcc
     9481 agacggttgg agttggagga gtttttcgaa ccgaccacca acacaacttc cgcctgttct
     9541 gccagggcgc gtaccgcttc ctgacggtta gtcgtggcgt agcagatgtc atctttgcgc
     9601 ggaccgacaa ttttcgggaa gcgtttacgc agcgcgtcga tcacatcaga cgtgtcatcc
     9661 accgacagcg tggtttgggt cataaaggag agcttctctt cgtttttgac cgtcagtttc
     9721 cacacatcgt ctggcgattc gaccagatac attccccctt cggggttgct gtactgcccc
     9781 atcgtccctt ccacttccgg gtgcccggcg tggccgatga gaatagattc ttcgccacgg
     9841 cgactggcgc gggcaacttc catatgcact ttggtcacca gcggacaggt ggcatcaaac
     9901 accgtcaaat cgcggctttt cgcttcgtta cgaaccgcct gagaaacacc gtgtgcggag
     9961 aaaatcagga tcgcgccgtc cggcacttcg ctaatctgct caataaagat agcaccacgc
    10021 tcgcgcaagc tatcgaccac gtagcggtta tgcaccactt cgtgacggac atatatcggt
    10081 gcgccgtaaa tggccagcgc gttttcaaca atgctgatag cgcggtctac cccggcacaa
    10141 aaaccacgcg ggttggccaa caggatctgc atgttacgcc tccagtgccg gatcgatttc
    10201 cagcacttca atatcaaaat gaacggtctg cccggccagc ggatggttga aatcaacggt
    10261 aatggagtcg ccgttaattt cgcggatcac accaggcatc tcactgccat ccattgcggt
    10321 aaaaagcatg attgcgccaa tttctggctc gcctgcatcc ataaattcac ggcgggagaa
    10381 gtactgaatc aggtccggtg acggcacgcc aaacgccgca tctggctcca gcgagaaggt
    10441 ggttttgtcg cccactttca gccccagcag gtgttgctcc agcccttcag aaagagaagc
    10501 atcgcccagg cggaacagcg ccggtttacc gttgttgcga gtagactcgg cggtggtgcc
    10561 atcgtcgagt tttagcgtga agtgcaccag gacggcgcta ttgctctgta cagattcaga
    10621 catgcaggtt tgctcttatt tttgaggatt tttgccggat gcggcctacg atttcgtagc
    10681 aatctgtagg ccgcatccgg cagggtttat tattgttttt tcgctttgga aggtaaaaac
    10741 ccttccagca cgatcagtgc cgcaccgaca caaatggcag tatcggcaag gttgaaggtg
    10801 gcgaagtgcc agtcgccgac gtagaagtcg atcatatcga caacgaagcc gtgccacagg
    10861 cggtcgaaca ggttgcccag cgcgccgcca ataatcagcg cgtaagcgat attgtttagc
    10921 ttctgcgtgg ccttcgagcg atacatcatc actgccagga gcacgctaat accaatcgca
    10981 ataccggcaa agaaccaacg ctgccagccg ccgctatcgg caaggaaact aaacgccgcg
    11041 ccatagttac gcgcataatg cagattaagc gacgggaaca gcgggaccgt atcccccagg
    11101 gcaaagttct ggaggatcag gtatttgctg cccagatcga taatcagcac gactaccacc
    11161 agccacagcc agcgtagccc tgttgaacag atcgattgac tcatcaggca aacttacgtt
    11221 tttcaccgtc accggcgacg ttgctgacac agcggccgca gatttctgcg tgttccgcca
    11281 ccttgccgac atcctgggtg tagtgccagc agcgtgggca cttctcacct tcggctttac
    11341 tcaacgcgac tttcagccct ttgagtactt cgctctgctg agcatcagca ggtgcgtcgt
    11401 tatagtctgc aacggtagcg ccggaggtca acaggacaaa tcgtaattca tcgcccagcg
    11461 cggtcagttt cgccgccagt tccggttcta catacaaggt tactgccgct tccagcgagc
    11521 cacccacttt cttgtcggca cgcgcttgct caatgacttt gttcacttcg ccacgcactt
    11581 tcaacagctc gtcccagaac gcatcgttca ttgcttcact gtctgccaga ccaaacaggc
    11641 cttcgtacca ctcaccggtg aagacgtatt tttcacgttc gcccggcaga tagccccaca
    11701 cttcatcagc ggtgaaggag aggattggtg ccatccagcg caccagcgct tcagcgatgt
    11761 gatacagcgc agtctggcag ctacgacgcg ccacgctatc cgctttggcg gtgtactgac
    11821 ggtctttgat gatgtcgagg tagaaggaac ccatctcaac ggagcagaag cgcatcagac
    11881 gctgtaccac ttcgtggaaa tcgtatgctt cgtacgcctt gaggatgtct tcctgtgccg
    11941 ctttcgcaca accaacggcc cagcgatcca gtaccaccat ctcttccggt ttcaccatat
    12001 cttttgctgg atcaaaacca ttcaggtttg ccagcaggaa gcgcgcggtg ttacggatac
    12061 gacgatagct atcggcagca cgtttcagga tctcgtcaga aacggccatt tcaccggtgt
    12121 agtcggttga tgccacccac agacgcagaa tatccgcacc cagtttgttc atcacatcct
    12181 gcggcgaaac ggtgttgccg atggatttag acatcttgcg accctgacca tccacggtaa
    12241 agccgtgggt cagtacctga cgatacggcg ctttgccttt catcgcggtg gagatcatta
    12301 gggaagacat gaaccaacca cggtgctggt cagaaccttc cagatacatg tccgctgcgt
    12361 gaccggcaaa ttccggacgc acgtcaacaa cagaagagtg ggtagatccg gagtcaaacc
    12421 atacgtcgag agtatccggc actttcacat actggtcagc ttcgtcgccg aggatctctt
    12481 tcgcatcgag atcccaccac gcctggatgc cgtcaacttc aacgcgttta gccacttctt
    12541 ccatcagttc gagggtacgc ggatgcagct cttccgtgtc tttgtgcacg aacagtgaca
    12601 tcggtacgcc ccaggtgcgc tgacgggaga tacaccagtc aggacggtta gcaaccatcg
    12661 actcgatacg cgcctggccc cagtccggga tccactgcac gcctttgatc tctttcagtg
    12721 actgcgcacg cagacctttc tgatccatgc tgacgaacca ctgcggtgtc gcgcggaaga
    12781 tgatcggcgt tttgtgacgc cagcagcacg gatagctgtg ctgcattttc tcaacgtgca
    12841 gcagcgcgcc tttttcctgc agcagcgcaa cgacgatgtc gttcgctttg aagacgttca
    12901 cgccatccag cgtcggataa gtgcccggca gataagtgcc gtccgggcca accgggttag
    12961 cggtttccag gccgtatttc tgaccgatca catagtcgtc cgggccgtgg ccaggcgcgg
    13021 tgtgaacggc accggtaccg gcatccaggg taacgtgatc gccgaggatt gccggaacgt
    13081 cgaagcccat aaacggatgg gtaaagcgca gcagctcaag ctctgcacct tttaccgtgc
    13141 cgagaatggt gtaatcggtc acgccgatac gctgcattac gctttcaacc agatctttcg
    13201 ccagaatcac ggcctgaccg tcgatctgca ccagcgcata gtcgaaatca ggtgcaatag
    13261 agattgcgcg gttcgcaggc agagtccacg gcgtagtggt ccagatcacc agcgagattg
    13321 ggccgttaac gttgcttacg ccaaatttcg ctttcagcgc atcctgatcg accgcctgga
    13381 aagcgacgtc aatggacgga gaagttttgt cgtaatactc aacttccgct tctgccagtg
    13441 cagaacggca gtctacgcac cagtgcaccg gcttcgcgcc tttgtgcagg tgaccattgc
    13501 cgatgatttt gcccagcgcg cggatgatgt tggcttcagt tttgaagtcc atagtcagat
    13561 acgggtgcga ccagtcgccc agcacgccca gacggataaa gtctttgcgt tgaccgtcaa
    13621 cctgggtcgc cgcgtattcg cggcacttgg cgcggaactc ggcggcggtg aatttctcac
    13681 ccggcttacc gtattcttgc tctactttca gctcgatcgg cagaccgtgg cagtcccagc
    13741 caggcacata cggcgagtca taaccggaaa gccctttgga cttcacgata atgtctttca
    13801 gaatcttgtt aaccgagtga ccaatatgaa tgctgccatt cgcataagga gggccatcat
    13861 gcagaatgaa ggtttttttg ccttttttag ccgcacggat gatgccgtac agatcatcat
    13921 cagtccaacg cgccagcatt cccggttcgc gcttggcgag atcgccacgc atcgggaacc
    13981 ctgtttccgg caaattcagg gttgatttat agtcactcat cagattctcg gttccgtgtt
    14041 tcggtttgat tacataacag gcttaagccg gttttgttag cccaaaaaat tcgcgggcgg
    14101 ttaattcatc acgcgcaatc tgcgctttca gttcgtccag cgacgcaaat cgctgctcat
    14161 tgcgtatttt tttacgcagc actacttgta tatggcgacc gtaaaggtcc attgcaacat
    14221 ctaacaaatg cacttccagc tgctggcgaa taccggcaac cgttgggcgt gttccgatgt
    14281 ttgccacgcc gggtaacggc ttttcaccga ggcccagcac ttctaccgca taaacccctt
    14341 tcaccgggga aacctgacgg cgtagcggta cattcgccgt cgggaaacct atagtgcgcc
    14401 ctaattcatc accgtggact acacgcccgg agatggcaaa cgggtgcccc agtaaacttt
    14461 ctgccagagc cagattgtca tcagccagcg cctgacgcac ggcggtgctg ctgatacgca
    14521 caccaccttc gcaaaaagtt tgcgtactgg tgatatcgaa gccgtattcc atgccagctt
    14581 tctgtaataa caagaaatcg ccttcacgac cagcgccaaa gcggaaatca tcacctacgg
    14641 caagaaattt tacgcgcaaa tgcttcacca aaagatcgct gatgaaattt tgcgcggtta
    14701 acgccgcaaa acgcctgtcg aaacgcacgc acagcacgta atcaacgcca cactctgcaa
    14761 ggtaacgcag tttttcccgc agccgggtca gtcttgccgg ggctttatcg gtagcaaaca
    14821 gttccagtgg ttgaggttca aaaagcatca ccatcaccgg taagttgcgc ttgcgccctt
    14881 cttcctgcaa gccctgtaac agcgcgcgat gaccgcgatg cacgccgtcg aaattaccaa
    14941 tagtcagcac acacccttct tgcggggcct ggctgagatt atgtatgccg cgtatcagct
    15001 tcatgtctgg ctcaaaacag tgaaaatcgt ccgagtatac cttgtacagc ggtcaaggtt
    15061 aaccggcgat tgagtaccga aatcagaaag aagtggcttt tcattgccat ggcgcaaatc
    15121 acgggaagaa actgaccgcc tgctgcaatt tttatcgcgg aaaagctgta ttcacacccc
    15181 gcaagctggt agaatcctgc gccatcacta cgtaacgagt gccggcacat taacggcgct
    15241 tatttgcaca aatccattga caaaagaagg ctaaaagggc atattcctcg gcctttgaat
    15301 tgtccatata gaacacattt gggagttgga ccttggctaa tatcaaatca gctaagaagc
    15361 gcgccattca gtctgaaaag gctcgtaagc acaacgcaag ccgtcgctct atgatgcgta
    15421 ctttcatcaa gaaagtatac gcagctatcg aagctggcga caaagctgct gcacagaaag
    15481 catttaacga aatgcaaccg atcgtggacc gtcaggctgc taaaggtctg atccacaaaa
    15541 acaaagctgc acgtcataag gctaacctga ctgcacagat caacaaactg gcttaatcgc
    15601 caatttgctg aagctttgtg aaaaagcccg cgcaagcggg tttttttatg cctgctgctt
    15661 ttgtgatgcg tgagagaaca cactgccggt tgccagatag tcaaaagaga catgccgttg
    15721 tactatctga actcttcgtg gcttaagaat cgtagaacca tcggctgata cattctgaac
    15781 aagaatatct actcaatctc cagactcccc cacaaagaat atggatattg tgatacacat
    15841 tgaggtatta aacttaagac cactctattt ttgaattaaa ttcctatata ctttaaccat
    15901 tcataaatat ctcctcatta gacc
//
LOCUS       DBKEKI010000085        15415 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.085, whole genome shotgun sequence.
ACCESSION   DBKEKI010000085 DBKEKI010000000
VERSION     DBKEKI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.085"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..809
                     /gene="dosC"
                     /locus_tag="ACUQPX_002734"
     CDS             <1..809
                     /gene="dosC"
                     /locus_tag="ACUQPX_002734"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HHV9057404.1"
                     /translation="VLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTF
                     SDSSASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQA
                     DFGLWFNHKGRHYFSGIAEVGHISRLIQDCDGIFNQTMRNTRILNNRSLRVKFLLQIR
                     NTVSQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLI
                     IDVDKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            734..1351
                     /locus_tag="ACUQPX_002735"
                     /pseudo
     CDS             734..1351
                     /locus_tag="ACUQPX_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            1609..1944
                     /gene="ddpX"
                     /locus_tag="ACUQPX_002736"
                     /pseudo
     CDS             1609..1944
                     /gene="ddpX"
                     /locus_tag="ACUQPX_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            1958..2854
                     /gene="ddpC"
                     /locus_tag="ACUQPX_002737"
     CDS             1958..2854
                     /gene="ddpC"
                     /locus_tag="ACUQPX_002737"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HHV9057405.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            2851..3837
                     /locus_tag="ACUQPX_002738"
     CDS             2851..3837
                     /locus_tag="ACUQPX_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHV9057406.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            3830..4756
                     /locus_tag="ACUQPX_002739"
     CDS             3830..4756
                     /locus_tag="ACUQPX_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HHV9057407.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKSTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTASAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(4812..5243)
                     /gene="osmC"
                     /locus_tag="ACUQPX_002740"
     CDS             complement(4812..5243)
                     /gene="osmC"
                     /locus_tag="ACUQPX_002740"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HHV9057408.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            5589..5804
                     /gene="bdm"
                     /locus_tag="ACUQPX_002741"
     CDS             5589..5804
                     /gene="bdm"
                     /locus_tag="ACUQPX_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HHV9057409.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            5906..6043
                     /gene="sra"
                     /locus_tag="ACUQPX_002742"
     CDS             5906..6043
                     /gene="sra"
                     /locus_tag="ACUQPX_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HHV9057410.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            6194..7891
                     /gene="maeA"
                     /locus_tag="ACUQPX_002743"
     CDS             6194..7891
                     /gene="maeA"
                     /locus_tag="ACUQPX_002743"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HHV9057411.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHSPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAAKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            8025..9035
                     /gene="adhP"
                     /locus_tag="ACUQPX_002744"
     CDS             8025..9035
                     /gene="adhP"
                     /locus_tag="ACUQPX_002744"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HHV9057412.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            9181..9465
                     /locus_tag="ACUQPX_002745"
     CDS             9181..9465
                     /locus_tag="ACUQPX_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HHV9057413.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(9693..10346)
                     /gene="fdnI"
                     /locus_tag="ACUQPX_002746"
     CDS             complement(9693..10346)
                     /gene="fdnI"
                     /locus_tag="ACUQPX_002746"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HHV9057414.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(10339..11223)
                     /gene="fdnH"
                     /locus_tag="ACUQPX_002747"
     CDS             complement(10339..11223)
                     /gene="fdnH"
                     /locus_tag="ACUQPX_002747"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HHV9057415.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(11236..14283)
                     /gene="fdnG"
                     /locus_tag="ACUQPX_002748"
     CDS             complement(11236..14283)
                     /gene="fdnG"
                     /locus_tag="ACUQPX_002748"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(13696..13698),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HHV9057416.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            14515..15396
                     /gene="yddG"
                     /locus_tag="ACUQPX_002749"
     CDS             14515..15396
                     /gene="yddG"
                     /locus_tag="ACUQPX_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HHV9057417.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGVHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSALLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         3737 a         3959 c         3913 g         3806 t
ORIGIN      
        1 gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc ggattattcc gccgcagaaa
       61 aacttcaggt ctaccatttc tcgattaaca gtattgatat cgcgatggaa gtgatgaccc
      121 gcgcgtttac ctttagtgac agtagtgcct caaaggaaga tgaaaactat cgtatcttct
      181 cgttactgga aaacgccgaa gaagaaaaag aacggcaaat agcctcaata ctttcatggg
      241 aaatagatat tatctataaa gtcctgctgg attctgattt aggcagcagt ttgcctttaa
      301 gccaggctga ttttggcctg tggtttaacc ataaaggtcg acattatttt agcggcattg
      361 ccgaagtggg ccatatctcc cgtctgattc aggattgcga cggtattttc aatcaaacca
      421 tgcgtaacac caggattttg aataacagaa gtttgcgggt gaaatttcta ttacagataa
      481 gaaataccgt atcgcagatt attaccttgc tgcgtgaatt gtttgaagaa gtatcgcgcc
      541 acgaagtcgg tatggatgta ctgacgaaat tacttaaccg ccgtttcctg ccgactatct
      601 tcaaacgcga aattgcccat gccaaccgga ccggtacacc gctgtcagtg ctgattattg
      661 acgttgataa attcaaagag atcaacgata cgtggggcca taacactggc gatgaaattc
      721 tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa atcgaaaata ttgggcgctg
      781 ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt agccagaata ttcatatccc
      841 ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt aatcagctgc ctaatcaggt
      901 gtctgatgca atgcaagcct ggggtattga cggccaccag ctgacggtgg aaatcacgga
      961 aagcatgatg atggaacacg ataccgaaat ctttaagcgc attcagatcc tgcgtgatat
     1021 gggcgtgggt ttatcggtag atgattttgg cacgggcttt tccggattat cccgcttagt
     1081 cagtcttccg gtaacggaaa tcaaaattga caaaagcttt gtcgatcgtt gtctgacgga
     1141 aaaacgcatc ctagccttac ttgaagccat taccagcatt gggcaaagcc tcaatttaac
     1201 cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag atgctacgta agatccactg
     1261 tcgcgttatt cagggatatt tcttttcccg cccccttccc gccgaagaaa ttccaggctg
     1321 gatgagcagc gtgttaccgc tgaaaatctg acaaattcct ctcgcccgca ctcgcgggtt
     1381 ttcctttaag gtgacactgt cacttaaata ctgtgatttc agctacgatt ccgggagatt
     1441 ccttccttaa ctttccctct tgcaccaaaa ctgcactacg ctgcacaaat gttgatcaaa
     1501 agttaaaaaa tataacttta agccagcatt ctggcgctta tcccggcatg gcatgagatc
     1561 tgcataagcg gaaagcgcag caatttttgt cttatacagg aaccgtttat gtcggatacc
     1621 accgaactgg ttgatttagc cgtgatcttc cctgatctgg agatcgaatt gaaatatgcc
     1681 tgcgttgata acatcacagg taaagctatt tatcagcaag cgcgttgtct gctacataaa
     1741 gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc agctgtcagg cttacaactg
     1801 gtgatttacg atgcgtatcg ccgcccacaa caagcacagg cgatgttgtg gcaagcctgc
     1861 ccagacccgc aatatgttgt tgatgtgacg gtcggttcta atcacagccg tggcacggcg
     1921 ctcgacctga cgcttcgtga tgacgtggag gtggtgaatg atgctaagcg aggaaacgtc
     1981 cgccgtacgc ccacaaaaac aaacgcgatt taacggtgca aaactggtat ggatgctgaa
     2041 aggcagtccg ctcaccgtga ccggcgcagt catcattgta ttaatgctac tgatgatgat
     2101 tttttcaccg tggctggcga cgcatgatcc caacgccatt gatttaaccg cccgcctttt
     2161 gccgccttct gcggcgcact ggtttggcac cgatgaagtg ggacgcgatc tgtttagccg
     2221 cgtactggtc ggcagtcagc aatcaattct cgccggatta gtggtggtcg ccattgcggg
     2281 tatgattggt tcgctactcg gatgtctatc cggtgtgctt ggcggacgcg cagacgccat
     2341 catcatgcgc atcatggaca ttatgctgtc gattccttcg ttggtactga caatggcact
     2401 ggctgccgct ctcgggccga gtttatttaa cgccatgctg gcgattgcta ttgtgcgaat
     2461 tcccttttat gtgcgcctgg cgcgggggca agcattagtt gtacgccagt atacctatgt
     2521 tcaggcggcg aaaacctttg gcgcgtctcg ttggcatctg atcaactggc atattttacg
     2581 taactcccta ccgccgctga tcgtgcaggc atcgctggat atcggtagcg cgattttaat
     2641 ggccgccacg ttgggattta ttggcctggg tgctcaacaa ccgagtgctg aatggggcgc
     2701 gatggtagcg aatggtcgca actatgtact cgatcagtgg tggtattgcg catttccggg
     2761 ggcagcgatt ttgcttaccg ccgtcgggtt taatctcttt ggtgatggta ttcgcgatct
     2821 gcttgacccg aaagcaggag gaaagcagtc atgacccaac ccgttctgga cattcaacag
     2881 ctgcatttga gtttccccgg ttttaacggt gatgttcacg cgctcaacaa tgtgtccttg
     2941 cagattaacc gcggcgaaat tgtcggtctg gtgggagaat ccggctcagg taaatcagtc
     3001 accgcaatgc tgattatgcg tctgctaccg acgggcagtt attgcgtaca tcgggggcag
     3061 atttcactgc tgggagaaga tgttcttaat gctcgggaaa aacagcttcg tcagtggcgc
     3121 ggcgcacgag tggcgatgat ctttcaggaa ccgatgaccg cccttaatcc gacacgtcga
     3181 ataggtcttc agatgatgga cgtaatccgc catcatcaac caataagtcg tcgggaagcc
     3241 agagctaaag cgattgccct gctggaagag atgcaaatcc cggatgccgt ggaagttatg
     3301 tcgcgctatc cgtttgagct ttcaggtggt atgcgccagc gggtaatgat tgcgctggca
     3361 ttctcctgcg agccgcaatt gattattgcc gacgaaccga cgacggcgct ggacgtcaca
     3421 gtacagttgc aggtactgcg tttgcttaaa cataaagcac gcgccagtgg aactgcggta
     3481 ctgttcatca gccatgatat ggccgtggtg tcgcaactgt gcgatagcgt ttacgtgatg
     3541 tatgccggaa gcgtaattga aagcggcggg acggcagacg ttatccatca tccccggcat
     3601 ccgtatacca ttggtttgct gcaatgcgca ccagaacatg gagtaccacg ccagccatta
     3661 cccgccattc cagggacggt accaaacctc acccatttgc ctgacggctg cgcttttcgc
     3721 gatcgttgct atgcggcagg tgcacagtgt gaaaacgtcc cggcgctgac agcgtgtggt
     3781 gacaacaacc agcactgcgc ctgttggtat ccgcagcagg aggtcataag tgtctgacac
     3841 gttattaacg ttacgcgacg tccatatcaa ttttccggcc cgtaaaaact ggcttggtaa
     3901 atctacggaa catgttcatg ccattaatgg tattgattta cagatccgcc gtggtgaaac
     3961 cttagggatc gtcggcgagt caggctgcgg caaaagcacc ctcgcacagc ttttaatggg
     4021 catgctgcaa ccgagccacg ggcagtacat ccgttcaggc tcacaacgca ttatgcagat
     4081 ggtgtttcag gacccgctct cttcgcttaa tccgcgctta ccggtgtggc gcatcatcac
     4141 agaaccgctc tggatagcta agcgtagtag tgaacaacag cggcgagcgt tggcagagga
     4201 gttggctgtg caggtgggta ttcgtccgga gtatctcgac cgcctgcctc atgcgttctc
     4261 cggcgggcag cggcaacgca tcgccattgc cagagcactc tcttcgcagc ctgacgtgat
     4321 tgtgcttgat gagccaacct cggcgctgga tatctccgtg caggcgcaga tcctcaattt
     4381 actggtaacg ctacaggaaa atcacgggct gacttatgtg ctgatttcac acaatgtctc
     4441 ggtgatacgt catatgagcg atcgggtggc ggtgatgtat ctcgggcaga ttgtagaact
     4501 gggcgacgcg caacaggtgt tgactgcttc ggcacatcca tacacccgat tattgctgga
     4561 ttccctcccc gccattgata aaccgctgga ggaagaatgg gcattacgta aaacggatct
     4621 gccgggaaac cgcacgttgc cgcaaggctg ttttttctac gaacgttgcc ctctggcaac
     4681 ccacggatgt gaagtccggc aatcattagc aataagggag gacggacgtg agctccggtg
     4741 ctggcgggcg ctgtagagat gctaacaacg catcaggcat tcacgcgcct gacgcgtcat
     4801 ccggcaatgc tttacgattt caactggtaa tccagcgtaa tttccgcttt cagtacctga
     4861 gagaccgggc atcctgcttt tgctttctgg atgatgccgt caaaggtaga ggcatcaata
     4921 cccggcaccg caacttcact cttcagtgcg attttcgtaa tcgcaaaacc ggcatccact
     4981 ttatccagcg acacatcggc ggtggtatca attgatgttg gcgtgaatcc cgcttccccc
     5041 agcattaatg aaagcgccat tgagaaacat gcggcatgcg ctgcgccaat cagttcttca
     5101 gggttggttc ctttttcgcc ttcaaaacgc gtgttaaatc catacggctg ttggttcagc
     5161 acgccgctct cggtggatac tgttcccttc ccgcgtttga tatcgccttc ccagtgtgcc
     5221 tgacctttct tatggattgt cattgttgct ctcctgtggg cttaaaaagg aaattataga
     5281 ctacgtgaga aacagcacga ggataagcag gatattccga ataaaatacg gcagaaaata
     5341 ttacttctgc acttaaatta aattcccaca atagataacc gggaatatat atctcaagag
     5401 atattgcatc aatatcaatc aactcaccac aataataata cctatcaata tcatggggtt
     5461 aaaatataaa aatcatcaac caggactaat ctcaacaacg aagcggtaaa tatttgccat
     5521 gctgaatgtg ctttataaat ctgcgatccg tagcagacac cataaataca cagacacgga
     5581 gaatcactat gtttacttat tatcaggcag aaaattcaac agcagaaccc gctctggtta
     5641 atgccattga gcaaggcctt cgggcagagc acggtgttgt cactgaagat gacattctca
     5701 tggagctgac caaatgggtg gaagcttctg ataacgacat cctcagtgat atctaccagc
     5761 aaacgatcaa ttatgtggtc agtggccagc accctacgct ttaaggtgct atgcttgatc
     5821 ggcaacctaa tttaggggtt tagcacgtgt ttcttcgcta cggcgatgtt gtccttaaaa
     5881 ctagcaacag gattgaggag ttaaaatgaa atcgaaccgt caggcacgtc atattcttgg
     5941 actggaccat aaaatttcta accagcgcaa aatagttacc gaaggcgaca aatccagcgt
     6001 ggtaaataac ccaaccggca gaaaacgccc cgctgaaaag taattcataa ccatcagtcc
     6061 tcaatgacga ttaaacacca ttgcctgcgc aatggtgttt ttatctgctt tatacttggg
     6121 gacgacgccc tggcggtaaa gcaaagacga taaaagcgtg ccagggatgg atattcaaaa
     6181 aagagtgagt gacatggaac caaaaacaaa aaaacagcgt tcgctttata tcccttacgc
     6241 tggccctgta ctgctggaat ttccgttgtt gaataaaggc agtgccttca gcatggaaga
     6301 acgccgtaac ttcaacctgc tggggttact gccggaagtg gtcgaaacca tcgaagaaca
     6361 agcggaacga gcatggatcc agtatcaggg attcaaaacc gaaatcgaca aacacatcta
     6421 cctgcgtaac atccaggaca ccaacgaaac cctcttctac cgtctggtaa acaatcatct
     6481 tgatgagatg atgcctgtta tttatacccc aaccgtcggc gcagcctgtg agcgtttttc
     6541 tgagatctac cgccgttcac gcggcgtgtt tatctcttac cagaaccgcc acaatatgga
     6601 cgatattctg caaaacgtgc cgaaccataa tattaaagtg attgtggtga ctgacggtga
     6661 acgtattctg gggcttggtg accagggcat cggcgggatg ggcattccga tcggtaaact
     6721 gtcgctctat accgcctgtg gcggcatcag cccggcgtat acccttccgg tggtgctgga
     6781 tgtcggaacg aacaaccaac agctgcttaa cgatccgctg tatatgggct ggcgtaatcc
     6841 gcgtatcact gacgatgaat actatgaatt cgttgatgaa tttatccagg ctgtgaaaca
     6901 acgctggccg gacgtgctgt tgcagtttga agactttgct caaaaaaatg cgatgccgtt
     6961 acttaaccgc tatcgcaatg aaatttgttc ttttaacgat gacattcagg gcactgcggc
     7021 ggtaacagtc ggcacactga tcgcagccag tcgcgcggca ggtggtcagt taagcgagaa
     7081 aaaaatcgtc ttccttggcg caggttcagc gggatgcggc attgccgaaa tgatcatcgc
     7141 ccagacccag cgcgaaggat taagcgagga agcagcgcgg cagaaagtct ttatggtcga
     7201 tcgctttggc ctgctgacgg acaagatgcc gaacctgctg cctttccaga ccaaactggt
     7261 gcagaagcgc gaaaacctca gtgattggga taccgacagc gatgtgttgt cactgctgga
     7321 tgtggtgcgc aatgtaaaac cagatattct gattggcgtc tcaggacaga ccgggctgtt
     7381 tacggaagag atcatccgtg agatgcataa acactctccg cgtccgatcg tgatgccgct
     7441 gtctaacccg acgtctcgcg tggaagccac accgcaggac attatcgcct ggaccgaagg
     7501 taacgcgctg gtcgccactg gcagcccgtt taatccagtg gtatggaaag ataaaatcta
     7561 ccctatcgcc cagtgtaaca acgcctttat tttcccgggc atcggcctgg gtgttattgc
     7621 ttccggcgcg tcacgtatca ccgatgagat gctgatgtcg gcaagtgaaa cgcttgctca
     7681 gtattcgccg ctggtcctga acggcgaagg tctggtacta ccggaactga aagatattca
     7741 gaaagtctcc cgcgcaattg cgtttgcggt tggcaaaatg gcgcagcagc aaggcgtggc
     7801 ggccaaaacg tctgctgaag ctttgcaaca agccattgac gataatttct ggcaagccga
     7861 ataccgcgac taccgccgta cctccatcta agcctgcacc cggtagtgaa ggctaccggg
     7921 ctatttccct ctcccttttt cagatctcat ccatactggg tagtggcgaa taaatctcat
     7981 ttgcctcacc tgctatgcag aacatcatcc gaaaaggagg aactatgaag actgcagttg
     8041 ttacgaagga tcatcatgtt gacgttacgg ataaaacact gcgctcactg aaacatggcg
     8101 aagccctgct gaaaatggag tgttgtggtg tatgtcatac cgatcttcat gttaagaatg
     8161 gcgattttgg tgacaaaacc ggcgtaattc tgggccatga aggcatcggt gtggtggcag
     8221 aagttggccc aggtgtcacc tcattaaaac caggcgatcg tgccagcgtg gcgtggtttt
     8281 acgaaggatg cggtcattgc gaatactgta acagtggtaa cgaaacgctc tgccgttcag
     8341 ttaaaaatgc cggatacagc gttgatggcg ggatggcgga agagtgcatc gtggtcgccg
     8401 attacgcggt aaaagtgcca gatggtctgg actcggcggc gcccagcagc attacctgtg
     8461 cgggggttac cacctacaaa gccgttaaac tgtcaaaaat tcgtcccggt cagtggattg
     8521 ctatctacgg tcttggcggt ctgggtaacc tcgccctgca atacgcaaag aatgtcttta
     8581 acgcaaaagt gatcgccatt gatgtcaatg atgagcagtt aaaactggca accgaaatgg
     8641 gtgcagattt agcgattaac tcacgcaccg aagacgccgc caaaattgtg caggagaaaa
     8701 ccggtggcgc tcacgctgcg gtggtaacag cagtagctaa agctgcgttt aactcggcag
     8761 ttgatgctgt ccgtgcaggc ggtcgtgttg tggctgtcgg tctgccgccg gagtctatga
     8821 gcctggatat cccacgtctt gtgctggatg gcattgaagt ggtcggttcg ctggtcggca
     8881 cgcgccagga tctaaccgaa gccttccagt ttgccgccga aggcaaagtg gtgccgaaag
     8941 tcgccctgcg tccgttagcg gacatcaaca ccatctttac cgagatggaa gaaggcaaaa
     9001 tccgtggccg tatggtgatt gatttccgtc actaagaggt ctttgctgcg actcccatgt
     9061 tcgggtcgca gcatcgcaca ctctccaaca tgaaatggct aaatggatta ttgttgcgta
     9121 ggaaggaaca ataatgatag tgttcccaga gaagtaacgc tactgagagt aaagacacta
     9181 atgaaaatgg ccaatcatcc ccgcccgggg gacattattc aggaatcact ggacgaactt
     9241 aatgtcagcc tgcgcgagtt tgccagagca atggaaattg cgccctcaac ggcaagccga
     9301 ttgctgaccg gaaaagcagc tttgacgcca gaaatggcaa ttaaactttc cgtggtgatc
     9361 ggcagttcgc cgcaaatgtg gctgaatctg caaaacgcct ggagtctggc agaggcagaa
     9421 aaaacggtgg ttgtgtcgcg attacgccgt ctggttacgc aataaaaaat ggcaccttat
     9481 aaagcgccat ttgaaactga tgacaaacgc aacattgcct gatgcgctac gcttatcagg
     9541 cctacacgtt cacttcaatc tattgtattt gaagggtttt catgccggat aaggcattca
     9601 cgacgcatcc gggatgaaca aagcccacgt tgtctacaat atgaaaatgg cgccttttac
     9661 agcgccattt tcagcttaaa gttcaaaaga gattatatcc cttcttcact ctcttttttc
     9721 gcttctgcct tctcgatttc acgataccag cgcggatggt gtttcttcgc ccagcgacgg
     9781 ctcaccttcc cttcgatcat ccctttaatc gaccctttca cccagaatgc catatacata
     9841 tggatcagga tggcgtggat caggatgata cccgcagccg cgtggatcag caggctgtag
     9901 cgaacaacct gcatcgggaa gtactgcgca aagtacggac gccagataat caccccggtc
     9961 accagcagca cgaaaatcat gctcatgatc gaccagaaca tcatcttttg cccggcgttg
    10021 tacttaccaa catccgccac tttgtgctca ttgcctttta atacttcgac aatgttcaac
    10081 agccacggaa tatctttctt atccgggatg ttgtgatgca caaaacgcac aaacataaac
    10141 atcagtgcga cgaaaatcgc aatgccgaag aacgggtgca aaatgcgtcc catctgcggc
    10201 gtaccgaagg tttgcgtcag ccattgcagc gtcgggaaga agaacgaaat cccggacagc
    10261 gccaccagga agaagcaaat caccacggtc cagtgacagg cgcgatcaat aaatttggtg
    10321 cgcacaatca ttttcgactt actcatgatg atcctcctcg tcatcgtcca cctccttatt
    10381 cgggccaata ccgatgtagt ggaaaatcaa cccggcaaaa gtggcaataa agccagccgc
    10441 tgccagcggt ttcaacgcgc ctttccacag gcttaccgag gtgtcgatct tcggatcttt
    10501 cggcagaccg tgatacagct ccggctgatc ggcgtgatgc agcacgtaca taacgtgcgt
    10561 accaccgacc ccttccgggt tgtagacgcc agcatgttcg taaccacgcg ctttcagttt
    10621 cgccacgcgc tgttccgcca gctccagcat ctccttcttg gtgccgaagt ggatagcccc
    10681 ggtcggacag gttttcacac aagccggttc ctggccgacg ctgacgcgat cgacgcagag
    10741 cgtgcattta tatacccggt tatcctcttt gttgaggcgc ggaatattaa acggacaccc
    10801 ggcaatgcag taaccacagc cgatgcagtt ttccgactgg aaatcgacaa tcccgttagc
    10861 gtactgaatg attgcaccag cagacgggca cgccttcagg cagcccggat cttcacagtg
    10921 catacagccg tctttacgga tcagccactc cagcttgcca ttctgttcgg tttcgctaaa
    10981 gcgcatcacc gtccaggact tggcgctcag atcggcgggg ttatcgtaaa ccccgacgca
    11041 gtgccccact tcatcacgga tgtcgttcca ctccgaacac gccacctgac aggctttaca
    11101 gccgatacag gtggaaacgt cgataagttt cgcgacttct gctttgtaat cacgcacctg
    11161 agaaggcggc gtgatggagt tagttgcgga ccttttgata atatcctgcg tttccatagc
    11221 catctgttcg cccccttacg ccttctcgat gttgactaag aacgctttat attccggcgt
    11281 ttgcgagttt gcatcaccga cattcggcgt cagagtgtta gcgatataac ctttacgcgc
    11341 gacaccctca aagccccagt ggattggaat acccaccgtt tcaacctgct gaccgtttac
    11401 attcagcggt ttcagacggc gcgttaccac tgccaccgcg cggataaagc ctcgcttgct
    11461 ggagacagtg acacgatcgc cattattaat gcctttcgcc gccgcctgcg tttcgctgat
    11521 ttccacaaac tgttccggct gagcaattgc gttgagcaac gcgtgcttgg tccaggtgtg
    11581 gaagtgctcg gtcagacgat aggtcgtacc cacatacggg aactgctctt ttttacccat
    11641 ccgcagcgcg tcttgttcat acagacgaac aaccgggtta gacaccacgt tcggatgcag
    11701 cgggttagtg cccagcggcg tttcaatcgg ctcgtagtgt tccgggaacg gaccttccgc
    11761 cattttgttg atggcaaaca ggcgtcccat cccttccggc tgcataataa acggcccggt
    11821 tggcgtaccc ggtgcggcat tgccgaagtc cggaatatcg ttacccgtcc acttgctgcc
    11881 gttccactgg atcagcatcc gtttcggatc ccacggttta ccgttgatat ccgccgaagc
    11941 acggttgtag agtacgcgac ggttgagcgg ccacgcccag gcccatccca gcgtattccc
    12001 cagaccggac gggtcggagt tatcgcggtt tgccatctgg ttaccctgct ctgtccagct
    12061 accggtgtag atccagcaag aagatgcggt tgtaccgtca tcacgcagat gcgcaaagct
    12121 actcagcaac tgacctttct tcgcaatcag cacgccatta gcatcataga gatcttccaa
    12181 cgcatagccg ttgttctctt tagctacttc gtcagattgc ggttcgtgcg gctgcttgta
    12241 gttccagctc atcttcatca gcggttctac gcctttacca ccttcggctt ggtacaactc
    12301 gcgcagatga tggtagatac ccgccagaat ttcgccgtcg ttacgcgctt cgcccggcgc
    12361 gtcctgacct ttccagtgcc actgcaacca gcgaccggag ttagcgatag aaccatcctc
    12421 ttcagcaaag caggtcgaag gcagacggaa tacttcagtc tgaatagacg ccggatcgac
    12481 atcgttcgac tcgccgtggt tctgccagaa ggtagaggtt tcagtcacca gcggatcgat
    12541 aaccaccatg tacttcagtt tgctcaggca gctcaccact ttgtttttat ccgggaagga
    12601 cgcaaccggg ttaaagccct ggcagaaata accggtgact ttgccttcat ccatcatgtt
    12661 gaaatacttg atgacgtcgt aggtctggtc ccacttcggc agccagtcat agccccagtt
    12721 gttctctttc tgcgcggcat cgccatagaa agatttcatc aggctaacga agaacttcgg
    12781 atagttgctc cagtagttca cctgatcagc cagcgtcgct ttcggcgtgt tcgcttccag
    12841 atacgactgc aaatcaacct gtttttctga cggcagcgtc agataacctg gcaggctggt
    12901 agagagcaga cctaagtcag tcagcccctg aatgttggag tgaccacgca atgcgttcac
    12961 gccgccaccg gccataccca tgttaccgag cagcaactgg atcatcgcca tagtacggat
    13021 gttctgcgca cccacggtgt gctgcgtcca gcccagcgcg tacaggaagg tggttgtgcg
    13081 atccggtgcg ctggtggagg ccagcacttc acacactttc aggaagtcgg cttttggcgt
    13141 accacagatg ttttcaacaa cgtccggcgt gtagcgggaa acgtgcgctt tcagcaggtt
    13201 ccacacacag cgcggatgag tcagtgtttc atcgcgtttc gcatagccgt tttcatcgaa
    13261 ctgatagttc caggacgatt tgtcgtactg gcgtttttca gcgtcataac cgctgaacag
    13321 gccatcttcg aaagcaaaat catcacgcac cagcaggctg gcgttggtgt aatgtttaac
    13381 gtattcggcg ttgattttgt tgttttcgat caggtagcgc aaaacgccag acaggaacgt
    13441 aatgtccgta ccggaacgaa taggtgcgta aatatccgcc acagaagcgg tacgcgtaaa
    13501 acggggatcg acaacgatca aggttgcatc gttgttgttt ttcgcttcca tcgcccagcg
    13561 gaaaccgacg ggatgcgctt cagcagcgtt accgcccatt accattacga cgttagcgtt
    13621 tttgatatcc acccagtggt tggtcatagc accgcgacca aatgttggag caagacttgc
    13681 taccgttggt ccgtgtcaga cgcgcgcctg gttgtctacc gccagcatcc cgagggagcg
    13741 ggcaaatttc tgggtcagca tcccggtttc gttgctggca ccggaggcac acagcatacc
    13801 ggtagaaagc caacggttta ccgttacgcc ctgctcgttc ttttcaataa agttagcgtc
    13861 acggtcagct ttcatcagct tcgcaatacg ggagaatgct tcttcccagc taatgcgctg
    13921 ccatttgtca gaacctggcg cacgatattc cgggtagcgc agacggtttt cactgttgac
    13981 gtaatccagc aaaccggccc ctttcgggca cagcgcacca cggcttaccg gatgatccgg
    14041 gtcaccttca atgtgataaa tcgcttctct ggcgtttttt gcgccatcac ccaggctata
    14101 catcaatagc ccgcaaccta cggaacagta tgtgcaggtg ttacggatct ctttagcgcg
    14161 taataatttg tagtttcgcg cctgagccag tgcttgcttc ggggcaaagc ccaatgccgc
    14221 taccgttgtt ccagccatac cgcccgcgca gattttaaaa aattgtctgc gactgacgtc
    14281 cattgctttc ctcttttttc aggggtatta ctgcgcgagg aaactaacag taaaattctg
    14341 aatctttttg cgcgaaatca aaaagaacac gtgtttgcca caaaataatc cgcctgcgca
    14401 aattgaccta cctcaatagc ggtagaaaaa cgcaccactg cctgacaggc cagttaaaaa
    14461 aatgctataa aattcagctt aatttttaac ggcaagagag acaaaacagc gagcatgaca
    14521 cgacaaaaag caacgctcat agggctaata gcgatcgtcc tgtggagcac gatggtagga
    14581 ttgattcgcg gtgtcagcga ggggctcggc ccggtcggcg gcgcagctgc tatctattca
    14641 ttaagcgggc tgctgttaat cttcacggtt ggatttccgc gtattcggca gattccgaaa
    14701 ggctatttac tcgccgggag tctgttattc gtcagctatg aaatctgtct ggcgctttcc
    14761 ttagggtatg cggcgacccg tcatcaggcg attgaagtgg gtatggtgaa ctatctgtgg
    14821 cccagcctga caattctctt tgccattctg tttaatggtc agaaaaccaa ctggctgatt
    14881 gtaccaggat tattattagc cctggtcggc gtctgctggg tgttaggcgg tgagaatggg
    14941 gtacattatg atgaaatcat caataatatc accaccagcc cattgagtta tttcctggcg
    15001 tttattggtg cgtttatctg ggcagcctat tgcacagtaa cgaataaata cgcacgcgga
    15061 tttaacggaa ttaccgtttt tgtcctgcta acgggagcat gtctgtggat ttactatttt
    15121 ctcacgccac aaccagaaat ggtatttagc acgcccgtca tgattaaact catctctgcg
    15181 gcatttacct taggatttgc ttatgctgca tggaatgtcg gtatattgca tggcaatgtc
    15241 accataatgg cggtaggctc gtattttacg cctgtacttt cctcagcgct tgcagccgtg
    15301 ctgctcagcg ccctgctgtc gttctcgttc tggcaaggcg cgctaatggt ctgcggcggt
    15361 tccctgctct gctggctggc gacacgtcgt ggttaaaata attgccgggt ctacg
//
LOCUS       DBKEKI010000086        15374 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.086, whole genome shotgun sequence.
ACCESSION   DBKEKI010000086 DBKEKI010000000
VERSION     DBKEKI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.086"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACUQPX_002750"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACUQPX_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HHV9057418.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="ACUQPX_002751"
     tRNA            complement(621..695)
                     /locus_tag="ACUQPX_002751"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(771..1703)
                     /locus_tag="ACUQPX_002752"
     CDS             complement(771..1703)
                     /locus_tag="ACUQPX_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HHV9057419.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTPHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1997..2752
                     /gene="mlaA"
                     /locus_tag="ACUQPX_002753"
     CDS             1997..2752
                     /gene="mlaA"
                     /locus_tag="ACUQPX_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HHV9057420.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2934..3993)
                     /gene="yfdF"
                     /locus_tag="ACUQPX_002754"
                     /pseudo
     CDS             complement(2934..3993)
                     /gene="yfdF"
                     /locus_tag="ACUQPX_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
     gene            complement(4359..5699)
                     /gene="fadL"
                     /locus_tag="ACUQPX_002755"
     CDS             complement(4359..5699)
                     /gene="fadL"
                     /locus_tag="ACUQPX_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HHV9057421.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6071..6355
                     /locus_tag="ACUQPX_002756"
     CDS             6071..6355
                     /locus_tag="ACUQPX_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HHV9057422.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6535..7845
                     /gene="fadI"
                     /locus_tag="ACUQPX_002757"
     CDS             6535..7845
                     /gene="fadI"
                     /locus_tag="ACUQPX_002757"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HHV9057423.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVLLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7845..9989
                     /gene="fadJ"
                     /locus_tag="ACUQPX_002758"
     CDS             7845..9989
                     /gene="fadJ"
                     /locus_tag="ACUQPX_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HHV9057424.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAALNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPFIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10192..10677
                     /gene="sixA"
                     /locus_tag="ACUQPX_002759"
     CDS             10192..10677
                     /gene="sixA"
                     /locus_tag="ACUQPX_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HHV9057425.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            11352..11915
                     /locus_tag="ACUQPX_002760"
     CDS             11352..11915
                     /locus_tag="ACUQPX_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9057426.1"
                     /translation="MSKFVKTAIAVAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            11997..14638
                     /gene="yfcU"
                     /locus_tag="ACUQPX_002761"
                     /pseudo
     CDS             11997..14638
                     /gene="yfcU"
                     /locus_tag="ACUQPX_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            14658..>15374
                     /locus_tag="ACUQPX_002762"
     CDS             14658..>15374
                     /locus_tag="ACUQPX_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HHV9057427.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTSYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNS"
BASE COUNT         3906 a         3553 c         3825 g         4090 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaattgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcatgagta tacccgctaa
      721 tggactgcgg ggtaagtacg ctgccgctcg attgcttaaa ccctcgccat ttatgctggg
      781 tttttatcat ttttcttaat gttttccgca cgttctgctt tttggcgtgc ttctgcttta
      841 cgcttgttgc tcatgtcgtt acgaatctgt gcatgactca ttaacgcgaa gataaaggtg
      901 ccgccgcaga tgttccccgc taaagtaggt agtgcgaagg gccagatgaa atcgctccag
      961 tgcagcgtac cgttaaacac cagatagagg atttcaacag aaccgaccac gatatggggg
     1021 gtgtcaccca gggcaataag ccaggtcatc aatataatca ccacaatctt tgccgcacct
     1081 gctgcaggaa acatccatac catagtggcg atcagccagc cggaaatgat cgcgttggca
     1141 aacatctcgc tgggggtgtt cttcatcaca tccatgccga ttttgacaaa tgcatcgcga
     1201 gtttcttcat tgaagatagg catatattca aatgcccacg cagcaatacc agtcccgaga
     1261 atattaccca gcagcacgac tccccataac cgcataagta agccaacgtt gctcattgtc
     1321 ggtttttgca tgacgggtag taccgcagtc acggtgtttt cggtaaataa ttgctggcgg
     1381 gccattatga cgataataaa accaaaggta taaccgagat tctccagcaa gaagctgccc
     1441 ggcacacctt ccagttcgac atgaaatatc ccttttgcca gtaacgaagc gcccatcgac
     1501 agacccgccg caatggctga ccacagtagc gccattgcgt cgcgttctag ctctttttca
     1561 ccatcctggc ggatatgctc atgaattgcc atcgcccggg aggggagtcg gtcttcatct
     1621 atttctattt ttttgccgcg ctctttttct tcgctctcaa cttcaatttc gtcgctgtgt
     1681 tgatcaattt tgtcgttgtc catggtctct tctcttgaat tagcacgtat agctaaagcg
     1741 tagcggcttt tttgctcgca actggcggga gttactctga aaatgtagaa aaggctgcgt
     1801 ttgccttttt ctgtttctat agaatcaagt agcctacagg gcggcgatta ccaggctatg
     1861 atcaaatcag caaatcaggg cgtctggaca tcagttgacg tgctgttaca atcgcccaca
     1921 cctaaacagg cggatacggt atcgttccgt catggatggc aaactgcata agccataaaa
     1981 aaacagggag acatttatga agcttcgcct gtcggcgctt gctctgggaa ctacgcttct
     2041 ggtggggtgt gcgagttccg gtacagatca gcaagggcgt tctgacccgt tagaagggtt
     2101 caaccgcacc atgtacaact tcaacttcaa cgtattagac ccgtatattg ttcgaccggt
     2161 cgctgtcgcc tggcgtgatt atgttccgca accggcgcgt aatggtttga gcaactttac
     2221 tggcaacctt gaagaacctg cggtgatggt taactacttc ttgcagggcg acccttatca
     2281 ggggatggtc cactttaccc gctttttcct gaacaccatt ttggggatgg gcggctttat
     2341 tgatgttgca gggatggcga acccgaaact gcaacggact gaacctcacc gcttcggtag
     2401 tacgcttggt cattatggcg tgggttatgg gccttacgtt cagttaccgt tctacggtag
     2461 cttcacgctg cgtgatgacg gtggtgatat ggcggatggt ctttacccgg ttctttcctg
     2521 gctgacctgg ccgatgtctg tgggtaaatg gacgcttgaa gggatcgaaa cccgcgctca
     2581 gctgctggat tccgatggtc tgctgcgtca gtcgtccgat ccttatatta tggtgcgcga
     2641 agcgtacttc cagcgtcatg atttcatcgc taatggcggc gaactcaaac cgcaggaaaa
     2701 tccgaacgcg caagcgattc aggatgattt aaaagatatc gattctgaat aagaaacaaa
     2761 taaaaaaggt gagtcgcaaa actcaccttt tttgttgtta tccctcaatt accacaagta
     2821 aaaggagata aaccgacaat gacccggacg taatgatgta aaaatactga tgttcgtcac
     2881 tgactatttc cgatattcag gcgttgaaaa atcaaaaaac tggaaaaata attttaaata
     2941 tcatcgaaat taacacctag tgcagatagt tgattaattt cattttgata tttcgcctgg
     3001 cgattttgaa gtcttttttc aactttatct tcgagatcct taaagtcttt gtatttattt
     3061 tcaggatggt caacgtcaac tttgttagcg aataaagtag aaattatttc ttttacatta
     3121 tcgcgtctaa attttgcatc gtccacttga aaattatatc ttattgctcg acctaaacgt
     3181 ttaaaaactt cgttaggttc tctcagtctt tccccgttga gctcgtttac aacctgatga
     3241 tcggaaccag ttgcaaatga ttctgccatg atagctttac tgctttcaaa ccacgctgaa
     3301 gcactcctgg cgttttcact acaatattgg gtgagactat caccattaat acgaattcct
     3361 ctttgaatgt ggtagtcgtc ctgatcgtag taaccgagag ataagtaatc atttccgttt
     3421 ttatttttaa ttacaatagc gagagagtcg ccttttttat ctttaccgta ccaggtaaat
     3481 aagtccgttg gttcgctttt cccgctctca tcaaattccg ttaccgtatg tctgagtttg
     3541 tgtgatatct tatcgaagat aaaaccagga tctccatcat atatttcttt taatgcgctg
     3601 aagatcattt ccttgttatt gatatcatga gtcttctcaa ctatgttgtc attaatgata
     3661 gtacggacga gttctttgtc tttatagttc gcaggtaatt ctattggttc ttggttgtcg
     3721 ataccaatag taatatggtc tgtatcattg gagccaatat caatataagt aactgaggaa
     3781 ttattaatat taaatctata aggatcgtcg tttactcgta tggcgttttt tataagctca
     3841 agtagatgtt taatacaggt gcttattcct tccttaccat taaaaatggt ttttgaactc
     3901 ttgtactaat ccattaattt catcattgtt attttcattg atggattctg ggtaagctgc
     3961 gctggtattg ttgattgaaa tagatggtag catgatttta ttccatgttg attgatttgt
     4021 tacaaatata tattacctaa tgaaatagac tatcaaaaaa cgctcattta tttagaacct
     4081 atctttaatt tgagtgtata agcgcacaat agccaggggg gttgagtatc ctggaaatgc
     4141 agaaaagtaa actctttgag aaggacgagc agaacgcttg aaaatatgca tttcccttca
     4201 ggtttgtctg tggataccac ttactgattt gaaagggcaa gctgccatca ggctgtttgc
     4261 aaaactcagg aggcaagcaa tgaggaaact actttcggtg aagtggggga gttaaagtca
     4321 cctgcatagc aggtgacttt atccaggcga acgcgttatc agaacgcgta gttaaagtta
     4381 gtaccgaaca gccaggcttt accttcagac tcgaactggt atgggccttc gttaattttc
     4441 acgctctgac cgtgcatata agaaacacca acgtcgactg aagcatcttt attaaatgcg
     4501 taagtcgtac ctgcactcag ccagaaacgg tcctggtccg gaatggagat agaacgattc
     4561 tgagccggaa ctgggctgtc atcaaaggcg ataccggtac ggaaggtcca gttatcatcg
     4621 tagtaataag tggtacccaa cgcgatgcgg taagcatctt taaagccttc atgtttctgg
     4681 aacagcgtgt cgccactggt tgaggtcgct ttcagctgct ggaactgact ccagctggtg
     4741 taagccaggc tatagtgaat cgcccactgt ggatcaacac ggttataacc tgacacttcc
     4801 cacatttcag gcaggttcag cgtcagataa cccgattgcg ttgcgccacc tgtcgcggta
     4861 ggaattggca aaccgtagtt attaaacgca cgattaagat cgctgctgta gttacctttg
     4921 aagtcaattt tcacttcaga acggtaggtc agtgcatagc ggttattttt atccagttca
     4981 tacaggattc cggcgttcca gccaaagccc cactggttac cgttcagatg agcgattttg
     5041 gtattactgt cgataccgtt ggcggtagct gccaatgctt gcccttgagg agtttgcccg
     5101 gcaggagatt gcataatctg accagcaacc aactgcccca gatcgcctgc gaaacgttca
     5161 attttcgcgc gagcgtagat ggcgttgaaa ccaagaccaa agctccatgc attatttaag
     5221 cgatacgcac cgcttaagtt caggttcatg gtttcaaggt cagttgtacc cccgacagag
     5281 ccgcctgcat aagtatcatt aaactctgtt gccagaccat agttagaggt aatagaagcg
     5341 ccccaaccaa attggtcgtt aatcggtgca acaaagtgca tgttcggaac ccatgccgta
     5401 ggcgcgatgt tatcggcttt caggctacga ccagacggag acgttccgct gatattcaca
     5461 tccgggtcaa tataaaccgc acccgcagaa aatgtcgggc ggtcaaacat ggtaatcaat
     5521 gcggggttac ggctaacgtt acctgcatca tcggcaattg cgccttcccc tgaataagcc
     5581 cggcccaggc cagaggaaga aaattcgttt aactgaaagc ctgccgacca ggcctgggtg
     5641 gagataagtg ccactgcgac tgcgagagca gactttgtaa acagggtttt ctggctcatg
     5701 accataacct caatgattta tttttataca aactatgtta cgtgctgtaa caggagcgcg
     5761 aagtgtaggg tctgaggtac atcttagaaa tcagaccagt ggcgagagta taggtcggac
     5821 cagctagaat gttgcaagta tgtttccatc ttttttcaaa tatgatctaa gaaacgcgat
     5881 ccagctggca aaatttggag gttgcgcact cgcggattcg ggtgaatttt gttttagatc
     5941 atttttaagt gtgatttcgg tcacttatcc gattttgcag aattaacaac tggagcagca
     6001 ctttccggaa gcgtaaaata tccacaacgt ttatctggca ccttgggtgc acatacagag
     6061 gaaatcaacg atgagtaaat gtagtgctga tgaaaccccg gtttgctgct gtatggatgt
     6121 tggcaccatt atggacaact ccgactgcac cgcgtcttac agccgcgtgt ttgcaaaccg
     6181 cgcagaagca gaacaaacgc tggcggcgct gactgaaaaa gcccgtagcg tggaatccga
     6241 accgtgcaaa atctccccga ctttcactga agagtctgac ggtgtgcgcc tggatatcga
     6301 tttcactttt gcctgcgaag ctgaaatgct gatcttccag ctgggtctgc gttaattcct
     6361 ctcgggtacg tacgcccctg tcagttgctg gcaggggcgt tttcttttct ttcttctgtc
     6421 ttcactgtgt catagctccc actttccttt ttctccgctt ggctaaatgt aaaaaaatgg
     6481 ttaagactat gatcaggtca gaccacttta tttatttttt tacaggggag tgttatgggt
     6541 caggttttac cactggttac ccgccagggc gatcgtatcg ccattgttag cggtttacgt
     6601 acgccttttg cccgccaggc gacggctttt catggcattc ccgcggttga tttagggaag
     6661 atggtggtag gcgaactgct ggcacgcagc gagatccccg ctgaagtgat tgaacagctg
     6721 gtctttggtc aggtcgtaca aatgcctgaa gcccccaaca ttgcgcgtga aattgttctc
     6781 ggtaccggaa tgaatgtgca taccgatgct tacagcgtca gccgcgcttg cgctaccagt
     6841 ttccaggcgg ttgcaaacgt cgcagaaagc ctgatggcgg gaactatccg agcggggatt
     6901 gccggtgggg cagattcctc ttctgtattg ccaattggcg tcagtaaaaa actggcgcac
     6961 gtgctggttg atgtcaacaa agctcgaacc atgagccagc gcctgaaact cttctctcgc
     7021 ctgcgtttgc gcgacttaat gcccgtaccg cctgcggtag cagaatattc tactggcttg
     7081 cggatgggcg acaccgccga acaaatggcg aaaacctacg gcatcacccg agaacagcaa
     7141 gatgcattag cgcaccgttc gcatcagcgt gccgctcagg catggtcaga cggaaaactc
     7201 aaagaagagg tgatgactgc ctttatccct ccttataaac aaccgcttgt cgaagacaac
     7261 aatattcgcg gtaattcctc gcttgccgat tacgcaaagc tgcgcccggc gtttgatcgc
     7321 aaacacggaa cggtaacggc ggcaaacagt acgccgctga ccgatggcgc ggcagcggtg
     7381 atcctgatga ctgaatcccg ggcgaaagaa ttagggctgg tgctgctggg gtatctgcgc
     7441 agctacgcat ttactgcgat tgatgtctgg caggacatgt tgctcggtcc agcctggtca
     7501 acaccgctgg cgctggaacg tgccggtttg acgatgagcg aactgacatt gatcgatatg
     7561 cacgaagcct ttgcagctca gacactggcg aatattcagt tgctgggtag tgaacgtttt
     7621 gctcgtgaag tactggggcg tgcacatgcc actggcgaag tggacgatag caaatttaac
     7681 gtgcttggcg gttcgattgc ttacggacat cccttcgcgg cgaccggcgc gcggatgatt
     7741 acccagacat tgcatgaact tcgccgtcgc ggcggtggat ttggtttagt taccgcctgt
     7801 gctgccggtg ggcttggcgc ggcaatggtt ctggaggcgg aataatggaa atggcatcag
     7861 cgtttactct taatgttcgt ctggacaaca ttgctatcat caccatcgac gtaccggatg
     7921 agaaaatgaa taccctgaag gcggaatttg cctcgcaggt gcgcgccatt attaagcaac
     7981 tccgtgaaaa caaagagttg cgcggcgtgg tgtttatctc cgcgaaaccg gacaatttta
     8041 tcgccggagc agacatcaac atgattggca actgcaaaac ggcgcaagaa gcggaagcac
     8101 tggcgcggca gggccaacag ttgatggcgg agattcatgc tttgcccatt ccggttatcg
     8161 cggctattca tggcgcttgc ctgggcggtg ggctggagct ggcgctggcg tgccacggtc
     8221 gcgtttgtac tgacgatcct aaaacggtgc tcggtttgcc tgaagtacaa cttggattgt
     8281 tacccggttc aggcggcacc cagcgtttac cgcgtctgat aggcgtcagt acagcattag
     8341 agatgattct caccggaaaa caacttcggg cgaaacaggc attaaagctg gggctggtgg
     8401 atgacgttgt tccgcactcc attttgctgg aagccgctgt tgagctggcg aagaaggatc
     8461 gcccatcttc ccgccctcta cctgtacgcg agcgtattct ggcggggccg ttaggtcgtg
     8521 cgctgctgtt caaaatggtc ggcaagaaaa cagaacacaa aactcaaggc aattatccgg
     8581 cgacagaacg catcctggag gttgttgaaa cgggattagc gcagggcacc agcagcggct
     8641 atgacgctga agctcgcgcc tttggcgaac tggcgatgac gccgcaatcg caagctttgc
     8701 gtagtatctt ttttgccagt acggacgtga agaaagatcc cggtagtgat gcaccgcctg
     8761 cggcattaaa cagcgtggga attttaggtg gtggcttgat gggcggcggt attgcttatg
     8821 tcactgcttg taaagcgggg cttccggtca gaattaaaga tatcaacccg cagggcataa
     8881 atcatgcgct gaagtacagt tgggatcagc tggagggcaa agttcgccgt cgtcatctca
     8941 aagccagcga acgtgacaaa cagctggcat taatctccgg aacgacggac tatcgcggct
     9001 ttgcccatcg cgatctgatt attgaagcgg tgtttgaaaa tctcgaatta aaacaacaga
     9061 tggtggcgga agtggagcaa aattgcgccg ctcataccat ttttgcttcg aacacgtcat
     9121 ctttaccgat tggtgatatc gccgctcacg ccgcgcgacc tgagcaagtt attggcctgc
     9181 atttcttcag tccagtggaa aaaatgccgc tggtagagat tattcctcat gcgggtacat
     9241 cggcgcaaac catcgccact acggtaaaac tggcgaaaaa acagggcaaa acgcccattg
     9301 tcgtgcgtga caaagccggg ttttacgtca atcgcatctt agcgccttac attaatgaag
     9361 ctatccgcat gctgaccgaa ggtgaacggg tagagcacat tgatgccgcg ctagtgaaat
     9421 ttggttttcc ggtaggccca atccaacttt tggatgaggt aggaatcgac accgggacta
     9481 aaattattcc tgtactggaa gccgcttatg gagaacgttt tagcgcgcct gcaaatgttg
     9541 tttcttcaat tttgaacgac gatcgcaaag gcagaaaaaa tggtcggggt ttctatcttt
     9601 atggtcagaa agggcgtaaa agcaaaaaac aggtcgatcc tgccatttac ccgttcattg
     9661 gcgcacaagg gcaggggcga ctctccgcac cgcaggttgc tgaacggtgt gtgatgttga
     9721 tgctgaatga agcagtacgt tgtgttgatg agcaggttat ccgtagcgtg cgtgacgggg
     9781 atattggtgc ggtatttggc attggttttc cgccatttct cggtggaccg ttccgctata
     9841 tcgattctct cggcgcgggc gaagtggttg caataatgca acgactggcc acgcagtatg
     9901 gttcccgttt taccccttgc gagcgtttgg tcgagatggg cgcgcgtggg gaaagttttt
     9961 ggaaaacaac tgcaactgac ctgcaataag aaggtcaaag ctatatgaat ccgcgctgaa
    10021 tggcggagtg ttggtcaaaa tgtaaacgca tattgactat acttacgcca ttgaggtaaa
    10081 aaacagcgtt tcattcggtg aatggataag gcacaatgcc ggccaccgtt ttctttctct
    10141 ggtttcagat gaaagaaaac gggcgaatct ggttaacaaa agcggtgcaa tatgcaagtt
    10201 tttatcatgc gtcacggcga cgcagccctc gatgccgcca gtgattcggt tcgtcctctg
    10261 accactaatg gttgtgacga atctcgcctg atggcgaact ggctgaaagg tcaaaaagtg
    10321 gaaatcgaac gcgttctggt gagcccgttc ctgcgagccg agcaaacact ggaagaagta
    10381 ggggattgtc tgaacctgcc ttccagtgca gaagttctgc cggagttaac gccctgcggc
    10441 gatgttggtc tggtcagtgc ctatttgcag gcgttgacca atgaaggtgt tgcttcggta
    10501 ctggtgatct ctcacttacc gttagtcggg tatctggttg ccgagttatg tccgggcgaa
    10561 acgccgccta tgtttaccac ctcggccatt gccagcgtaa cgcttgatga aagcggaaac
    10621 ggtacattca actggcagat gagcccgtgt aatctgaaga tggcaaaagc tatctgatgt
    10681 gttccaaatt atgcccaatg aggtagattc attgggcata attgctcatt aggcagcgga
    10741 tatctgtcaa cctcactact attgaagact ctccttatct ataaataaaa cactttctac
    10801 attcattcgg aaattattct tctgcgcgta tcttgatttt gcccaaagtt acatgttgaa
    10861 atgaataatg tctgtttgac actcattgtc atttattaat acagataata gagttgaata
    10921 atcaggcagg atgtaattga aacttgatta aattctggct atagtgttta tatattatat
    10981 tgtcaaaagt ggataaatgc caatcgcatt atatgttcat tgtttgtttt ttaagctgtt
    11041 gatattcatt attgatatac tttctatttt ttctaaatac ttattccgta ttttttaagg
    11101 atgtccttaa gataaccttt cttctgtcaa tatattaatt aaatctacat ttactcttta
    11161 gccaaattaa attggctcag gggtgtggaa acatcactat gcggccccac accaacgaat
    11221 acaattttct acgacctgtt ctttttgggt cgcatgccgt attcaatggc tgggtataaa
    11281 cattggctat tgactgaatc aatgtattgc aatgtttata tttttaatta tttatagttt
    11341 aaggacaaat aatgagtaag tttgttaaaa cagctattgc tgtggcaatg gtgatgggcg
    11401 tgttcacctc tacggcaaca atcgctgcgg gtaacaacgg tactgcacgt ttttacggca
    11461 ccattgaaga ctcagtgtgt tctatcgttc cggacgatca caaactggaa gttgatatgg
    11521 gtgatatcgg tgccgaaaaa ctgaaaaata acggcaccac cacgccgaaa agtttccaga
    11581 ttcgtctgca agattgcgta tttgatactc aggaaacaat gaccactacc tttactggta
    11641 ccgtttcttc tgcaaatagc ggcaattatt acaccatttt caataccgat accggtgcgg
    11701 catttaacaa tgttagcttg gcgatcggtg actctctggg tacctcttac aaaagcggca
    11761 tgggtattga ccagaaaata gtgaaagaca cttctaccaa caaaggcaaa gcgaagcaga
    11821 cactgaactt taaagcctgg ctggtcggcg cagctgatgc gccagatctg ggtaattttg
    11881 aagcaaatac caccttccag attacttacc tgtaaggcat actcccatag cgacactgct
    11941 atgggggatt taaatggatt ttgaccgaac aagaataaaa acggatagaa acgtgtatgc
    12001 ctgaccattc tctttttcga ttacggattc ttccgtggtg cattgcgctg gcaatgtcag
    12061 ggagttatag cagtgtctgg gctgaagacg acattcagtt tgattcccgt tttctggaat
    12121 taaaaggcga cacaaaaatt gatctgaagc gtttttccag ccagggatat gttgagcccg
    12181 gaaaatacaa tttacaggtt caactaaata aacagccatt ggcggaagag tacgatattt
    12241 actggtatgc tggtgaagat gacgcgagca aaagctatgc ttgtctgaca ccggaactgg
    12301 tagcgcagtt tggtttaaaa gaagacgtgg cgaaaaatct gcaatggagc cacgatgcta
    12361 aatgcctgaa atccggtcaa ctggaaggca tggaaattaa ggctgattta agccagtccg
    12421 cattagtcat ttcactgcca caggcttacc tcgaatatac ttatcccgac tgggatccgc
    12481 cttcacgttg ggatgacggc atctccggga tcgtcgcgga ctacagcatc aacgcacaaa
    12541 cccggcacga agaaaatggc ggtgatgata gtaacgagat cagcggcaac gggacggtcg
    12601 gggttaacct ggggccgtgg cgtatgcgtg ctgactggca gactaactat caacatactc
    12661 gcagtaatga tgacgatgaa ttcagcggcg atgaaactca aaaaaaatgg gagtggagtc
    12721 gctactatgc caggcgggcg ttaccatcat taaaagccaa actggcgctg ggcgaggatt
    12781 acctcagatc cgatattttt gatggtttta actatgttgg tggcagtgtc agtactgacg
    12841 atcaaatgtt gcctcccaat ctgcgcggct acgcgccaga catttccggc gtggcacaca
    12901 ccacagcaaa agtgaccgtc agccagatgg ggcgtgtgat ttacgaaacg taggttccgg
    12961 ctggaccgtt tcgtattcag gatcttggtg attctgtctc cggtacgttg catattcgca
    13021 ttgaagaaca gaacggccag gtgcaggaat atgacatcag caccgcctcg atgccatacc
    13081 tcactcgtcc aggtcaggtt cgctataaga tcatgatggg ccgtccgcaa gagtggggac
    13141 accatgtcga gggtgaattt ttttctggtg ctgaagcttc ctgggggatc gctaacggct
    13201 ggtcgttata tggcggcgca ctgggagatg aaaactatca gtctgcggcg cttggcgtcg
    13261 gtcgcgattt gtctacattc ggcgcggtcg cgtttgatgt tactcactcg cacaccaaac
    13321 tggataaaga caccgcttat ggcaaaggtt cgctggacgg taactccttc cgtgtgagtt
    13381 attccaaaga ctttgaccag ctcaacagcc gcgttacttt tgctggatat cgcttctcgg
    13441 aagagaactt tatgaccatg agcgagtatc tggatgccag tgacagcgga atggtacgca
    13501 cgggcaacga caaagagatg tacaccgcca cttataacca gaacttccgc gatgcgggtg
    13561 tttcggttta tctcaactat acccgccata cctactggga tcgcgaggag cagacaaact
    13621 acaacatcat gctctcgcac tatttcaata tgggcagtat tcgtaatgtc agcatctcga
    13681 tgactggcta ccgttacgag tatgacaacc aggccgacaa aggcatgtac atttcgctca
    13741 gtatgccgtg gggcgacaac agtaccgtta gctataacgg tactatggca gtgggacgga
    13801 cagcagtcag gtcggttatt tcagccgtgt cgatgacgcg actcactatc agttgaacgt
    13861 cggcaccagt gacaaacaca ccagcgttga cggctattac agccatgatg gttcgctggc
    13921 gcaggttgac ctcagtgcga actaccatga agggcaatac acctctgcgg gcttgtcgtt
    13981 acagggcggc gcaacgctta ctgcccacgg tggcgcactc caccgtaccc agaatatggg
    14041 cgggacacgc ttgttgattg atgccgatgg cgttgccgat gttccggtag aaggtaacgg
    14101 tgctgctgtt tataccaata tgtttggtaa agccgtcgtt tctgacgtca ataactatta
    14161 ccgcaatcag gcgtatatcg acctcaacaa actgccggaa aacgccgaag caacccagtc
    14221 ggtggtacaa gccacgctaa ctgaaggggc gattggctac cgcaaatttg ccgtcatcag
    14281 tggtcaaaaa gcgatggcgg tgctgcgttt acaagacggc agccatcctc cgtttggcgc
    14341 agaagtaaaa aatgataacg agcagacagt gggccttgtc gatgatgacg gcaatgttta
    14401 tctggctggg gtgaaacctg gcgaacacat gagtgtgttc tggagtggtg ttgcgcattg
    14461 cgatatcaac ctgccggacc cgctgcctgc cgatctgttt aacggcttgt tactgccatg
    14521 ccagcataaa ggcaacgtcg cgccggttgt tcctgatgac ataaagcctg tcattcagga
    14581 gcagacgcaa caggtgacac ccaccaatcc ccctgtttct gtttcagcta accaataacg
    14641 tgattaagga atgatccatg tctgatttac tttgctctgc caaattgggt gctacgacgc
    14701 tagcgttact gcttagcgct gccagtttgt ctgcccaggc ttctgtaacg ccagatcgta
    14761 cccgtttgat atttaatgaa agcgacaaat caatcagcgt gacgttgcgc aataacgatc
    14821 cgaaactgcc ttatctggcc caaagctgga tcgaagatga aaagggtaac aagatcagtt
    14881 caccgttaac tgttctgcca ccggtgcaac gcatcgattc aatgatgaac gggcaggtaa
    14941 aagtgcaggg aatgccggat atcaacaaat tgcctgccga tcgtgaaagc ctgttctact
    15001 tcaacgtgcg tgagatcccg ccaaaatcaa acaaagccaa cacgctgcaa atagcactac
    15061 aaacgcgtat taagttgttc tggcgaccga aggcgctgga gaacgtcagc atgaaaaacc
    15121 cctggcaata taaggttact ttaacccgca acggtcagga atttaccgtc aataacccga
    15181 cgtcgtatta cgtcattttc agtaatgcca gcacacagaa aaatggcaat ccggcagccg
    15241 gtttctcgcc aatagtgatg tcgccgaaaa cctctgcacc gctgaatgtg aagatgggta
    15301 gcgtgccggt actgacctat gtcaacgact atggcgctcg gatgccgctc tttttcactt
    15361 gcaacggtaa cagt
//
LOCUS       DBKEKI010000087        15278 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.087, whole genome shotgun sequence.
ACCESSION   DBKEKI010000087 DBKEKI010000000
VERSION     DBKEKI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.087"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..135
                     /locus_tag="ACUQPX_002763"
     CDS             <1..135
                     /locus_tag="ACUQPX_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HHV9057428.1"
                     /translation="VFAGVRLYMPALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW
                     "
     gene            145..783
                     /locus_tag="ACUQPX_002764"
     CDS             145..783
                     /locus_tag="ACUQPX_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HHV9057429.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNSYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            786..1949
                     /locus_tag="ACUQPX_002765"
     CDS             786..1949
                     /locus_tag="ACUQPX_002765"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HHV9057430.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2033..3658
                     /locus_tag="ACUQPX_002766"
     CDS             2033..3658
                     /locus_tag="ACUQPX_002766"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HHV9057431.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASSPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3775..4050)
                     /gene="yjfN"
                     /locus_tag="ACUQPX_002767"
     CDS             complement(3775..4050)
                     /gene="yjfN"
                     /locus_tag="ACUQPX_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HHV9057432.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4199..4528)
                     /gene="bsmA"
                     /locus_tag="ACUQPX_002768"
     CDS             complement(4199..4528)
                     /gene="bsmA"
                     /locus_tag="ACUQPX_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HHV9057433.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4710..5459
                     /gene="yjfP"
                     /locus_tag="ACUQPX_002769"
     CDS             4710..5459
                     /gene="yjfP"
                     /locus_tag="ACUQPX_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HHV9057434.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGALMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5456..6211)
                     /gene="ulaR"
                     /locus_tag="ACUQPX_002770"
     CDS             complement(5456..6211)
                     /gene="ulaR"
                     /locus_tag="ACUQPX_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HHV9057435.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6319..7383)
                     /gene="ulaG"
                     /locus_tag="ACUQPX_002771"
     CDS             complement(6319..7383)
                     /gene="ulaG"
                     /locus_tag="ACUQPX_002771"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HHV9057436.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGKALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7738..9135
                     /gene="ulaA"
                     /locus_tag="ACUQPX_002772"
     CDS             7738..9135
                     /gene="ulaA"
                     /locus_tag="ACUQPX_002772"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HHV9057437.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDNYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9151..9456
                     /gene="ulaB"
                     /locus_tag="ACUQPX_002773"
     CDS             9151..9456
                     /gene="ulaB"
                     /locus_tag="ACUQPX_002773"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HHV9057438.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9466..9930
                     /gene="ulaC"
                     /locus_tag="ACUQPX_002774"
     CDS             9466..9930
                     /gene="ulaC"
                     /locus_tag="ACUQPX_002774"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HHV9057439.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9944..10594
                     /gene="ulaD"
                     /locus_tag="ACUQPX_002775"
     CDS             9944..10594
                     /gene="ulaD"
                     /locus_tag="ACUQPX_002775"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HHV9057440.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTATGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10604..11440
                     /gene="ulaE"
                     /locus_tag="ACUQPX_002776"
     CDS             10604..11440
                     /gene="ulaE"
                     /locus_tag="ACUQPX_002776"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HHV9057441.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ECLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11440..12126
                     /gene="ulaF"
                     /locus_tag="ACUQPX_002777"
     CDS             11440..12126
                     /gene="ulaF"
                     /locus_tag="ACUQPX_002777"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HHV9057442.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12255..12530)
                     /gene="yjfY"
                     /locus_tag="ACUQPX_002778"
     CDS             complement(12255..12530)
                     /gene="yjfY"
                     /locus_tag="ACUQPX_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HHV9057443.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12857..13252
                     /gene="rpsF"
                     /locus_tag="ACUQPX_002779"
     CDS             12857..13252
                     /gene="rpsF"
                     /locus_tag="ACUQPX_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HHV9057444.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTVAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13259..13573
                     /gene="priB"
                     /locus_tag="ACUQPX_002780"
     CDS             13259..13573
                     /gene="priB"
                     /locus_tag="ACUQPX_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HHV9057445.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13578..13805
                     /gene="rpsR"
                     /locus_tag="ACUQPX_002781"
     CDS             13578..13805
                     /gene="rpsR"
                     /locus_tag="ACUQPX_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HHV9057446.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13847..14296
                     /gene="rplI"
                     /locus_tag="ACUQPX_002782"
     CDS             13847..14296
                     /gene="rplI"
                     /locus_tag="ACUQPX_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HHV9057447.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEVRRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14367..14936)
                     /locus_tag="ACUQPX_002783"
                     /pseudo
     CDS             complement(14367..14936)
                     /locus_tag="ACUQPX_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            14994..15278
                     /locus_tag="ACUQPX_002784"
                     /pseudo
     CDS             14994..15278
                     /locus_tag="ACUQPX_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3591 a         3722 c         4118 g         3847 t
ORIGIN      
        1 gtttttgctg gtgtcagact ttatatgccc gcattaagcg aaaaaattat taatcacaat
       61 accgcagcag gaatgtttat gggaaccgcc gcgctggctg gcggtatttt taacgcagct
      121 tgtatgacat ggtaatggac gatcatggcc agaaaacgca aatcaagaaa taacagtaaa
      181 attggtcacg gagcgatcag tcgcattggt agaccgaata atccttttga accgcgtcgc
      241 aatagctacg cacaaaaata cttaacgttg gcgctaatgg gcggtgccgc ttttttcgta
      301 ttgaaaggtt gtagcgatag tagcgatgtc gataacgacg gcgacggaac gttttacgcg
      361 acagtgcagg attgtattga tgacggtaat aatgccgaca tttgcgcgcg tggctggaac
      421 aacgccaaag cggcatttta tgccgatgtt ccaaagaata tgactcagca gaactgtcag
      481 tctaagtacg aaaattgcta ctacgacaat gttgaacaga gttggatccc ggtcgtttct
      541 ggatttttgt taagccgggt tattcgtaaa gatcgcgatg agccgttcgt ctataacagc
      601 ggcggatcct catttgcttc ccgccccgtc tggcgcaaca cttccggtga ttactcctgg
      661 cgcttcggtt ctggcaaaaa agagtcttac tcttcaggcg gctttaccac cagaaaagcg
      721 tcgaccgttt cgcgcggcgg ctatggtcgt tcttccagcg cccgtgggca ttggggaggc
      781 taatcatgct gagacacaac gttcctgtgc ggcgggatct ggaccagatc gccgctaaca
      841 acggtttcga ctttcatatc atcgacaatg aaatctattg ggacgagagt cgggcttacc
      901 gttttactct gcgccagatt gaagagcaga tcgaaaaacc gactgcggaa ctgcatcaga
      961 tgtgccttga ggtggtggat cgcgcggtta aagacgaaga gatcctgacg caactggcga
     1021 tcccgccgtt gtactgggat gtgatcgccg aaagctggcg cgcacgcgat ccttcgctgt
     1081 atggccggat ggattttgcc tggtgtggca atgcgccggt gaagctgctg gagtacaacg
     1141 ccgatacgcc gacctcgttg tatgagtcgg cttatttcca gtggttgtgg ctggaggatg
     1201 cccggcgcag cggcattatt ccgcgtgatg ccgatcagta caacgccatt caggaacgcc
     1261 tgatttcgcg ctttagtgag ctttacagtc gggaaccgtt ttatttttgc tgctgtcagg
     1321 acaccgatga agacaggagt accgtgctgt acttgcagga ctgcgcccag caggcagggc
     1381 aggagtcgcg gtttatctac attgaagatc tcggtttggg cgtcggcggc gtactgaccg
     1441 atcttgatga taatgtcatc cagcgtgcat ttaagctgta tccgctggag tggatgatgc
     1501 gtgacgataa cggtccgctg ctgcgcaagc gtcgcgagca atgggtggag ccgttatgga
     1561 aaagtatctt gagtaataaa gggctaatgc cgctgctttg gcgcttcttc cctggtcatc
     1621 ctaaccttct tgcatcctgg tttgagggtg aaaaatcgca gattgccgct ggcgaaagct
     1681 atgtgcgtaa accgatctac tcgcgcgaag gcggaaacgt caccattttc gacggtcaga
     1741 ataacgtcgt tgaccacgct gatggtgatt acgccgatga accgatgatc taccaggcgt
     1801 ttcaacctct gccgcgattt ggcgatagct acacactcat cggtagctgg attgtcgatg
     1861 atgaagcgtg cggaatgggg atccgtgaag ataacacact gatcaccaaa gacacctcac
     1921 gtttcgttcc gcattacatt gctggataag aatgttttag caatctcttt ctgtcatgaa
     1981 tccatggcag tgaccatact aatggtgact gccattgatg gagggagaca cagtgcactg
     2041 gcaaactcac accattttta atcaacctat accattaaat aacagcaact tatacctgtc
     2101 tgatggcgcg ctttgcgaag cggtaacgcg tgaaggtgct ggctgggata gcgattttct
     2161 agccagtatt ggtcagcagt taggaacggc tgaatccctt gaactggggc ggctggcgaa
     2221 tgtaaacccg cctgaactgt tgcgctacga tgcccagggg cggcgtctgg acgatgttcg
     2281 tttccatccc gcatggcacc tgctgatgca ggcgttatgt accaatcggg tgcacaatct
     2341 tgcctgggaa gaagacgctc gctccggcgc atttgtggca cgcgcggcgc gttttatgtt
     2401 acatgcgcag gttgaggctg ggtcgttatg tccaataacg atgacctttg ccgccacgcc
     2461 attgttgtta cagatgttac ccacgccgtt tcaggactgg accacgccgc tgctgagcga
     2521 tcgctacgat tctcacttat tgccaggtgg gcaaaaacgc ggtttgttga ttggcatggg
     2581 aatgacggaa aagcagggcg gttccgatgt catgagcaac accacccgtg cagagcgttt
     2641 ggaagatggc tcttatcggc tggtggggca taaatggttt ttctcggtgc cgcaaagcga
     2701 tgcgcatctg gtgctggcgc agaccgcggg cggtctgtcc tgcttttttg tgccgcgctt
     2761 tttgcctgac gggcaacgca acgcgattcg cctcgagcgg ctgaaagata agctgggtaa
     2821 tcgctctaac gccagttgcg aagtggagtt tcaggatgcc attggctggt tattggggca
     2881 ggaaggggaa ggaattcgtc tgatcctgaa aatggggggg atgacgcgtt ttgattgcgc
     2941 cctgggtagc catgccatga tgcgccgtgc attttcgctg gcgatttatc atgcacatca
     3001 acgccatgtt tttggtaatc cattgatcca acagcccctt atgcgtcatg tcttaagtcg
     3061 catggcactt cagcttgaag ggcaaacggc gttgctgttt cgtcttgcgc gagcgtggga
     3121 ccggcgtgcc gatgccaaag aagccctgtg ggcgcgttta tttacgcctg cggcaaaatt
     3181 tgtgatctgc aaacgaggta tgccgtttgt ggccgaagcg atggaggtgc tgggcggcat
     3241 tggttattgc gaggagagcg agctgccgcg gctttaccgg gagatgccgg taaacagtat
     3301 ttgggaaggt tccggcaata ttatgtgcct ggatgtgttg cgcgttctca ataagcaagc
     3361 gggcgtatac gacttattgt cggaagcatt tgtggaagtg aaagggcagg atcgctattt
     3421 tgatcgcgcg gttcgtcgtt tacagcagca gctgcgtaag ccagctgaag aactggggcg
     3481 agagattact catcagctat tcctgctggg ctgcggtgcg caaatgttga aatatgcttc
     3541 ttcgccaatg gcgcaggcgt ggtgtcaggt gatgttagat acgcgcggcg gcgtacggtt
     3601 gtcagagcag atccagaatg atttattgct gcgggcgacg gggggagtgt gtgtgtaagc
     3661 gtatacgact gatgcgacgc tggtttcgat taactaaatg aaatatgtga aaattgtagg
     3721 ccggacaagg cgctcgcgcc gcatccggca ttgttcattt gctgtagatg acactcatgc
     3781 atacagtatc gcctgtacgc gccagttatc aggctgctgg tcttcgtaca tggtaataat
     3841 gcggtaccat gacgcacctt gttcatcggc ctttaacgcc acgatacgtt ccacgtcctg
     3901 cggatcccct gaaatattgc taactgagat ctgacctatt tcattcaggc ccgtcacgca
     3961 gtcagcactg gcgaattctg cagactgtgc cgtggcgctc gcaggataag tcactaattg
     4021 cagcgagggt gaggcaagta attgtttcat cgtcagctcc atttttcgtt tccccacatg
     4081 ttgcagggca ttccatcgcg gtaataaacg cccaagcttc cagccggtaa tttattgtgc
     4141 acaggtaaac gcatagggac gcaaagcaat gtaaagctga ctatgtaagt tgtctgattt
     4201 atttacggta caaaatggct tgtgaatacc actgtcctgt cacgatggtt tcgtccacca
     4261 tgacgacaac gtaataatca gcttttgcag cgacagcttt tgctctgatt tctgctaatg
     4321 cgtcatccgg agaaccccga accatcgtgc ttacgctacc tattcgctgt aacccttgcg
     4381 tctggtcgcg gcgaatctct tgcggatggt ccgttactgg cggtgctggc tgtggcgtac
     4441 cttgcagtgc gctacaggca cttaacatca acaccaataa taaactggca aaccggtaaa
     4501 taacgctatt acgtttcctg ctaaccatag tgtagtgcct tattaattta actttggggg
     4561 aatgttcccg aattgttacc tggtacgcga ttttcgaatg aaaacgtgac gaaagcgtaa
     4621 cccacatctc aacattatga actaataccg ccagcagaaa cttgtgctaa ggatttcttc
     4681 atgtcatacc agacaaaaag gagagacaga tgattgaaat agaatcacgc gagctggcag
     4741 atattcccgt tcttcatgct tatccagtcg ggcaaaaaga taccccgtta ccgtgcgtaa
     4801 ttttttatca cggctttact tcatccagcc tggtgtatag ctattttgcc gttgcgctgg
     4861 cgcaggctgg tttgcgggtg atcatgccgg atgcgcccga tcacggtagc cgttttagtg
     4921 gcgacgaagc gcggcggtta aatcaattct ggcaaatctt gctacaaagt atgcaggaat
     4981 tcactacttt acgtgcggca atagccgaag aaaactggct gcttgatgac cgtctggcag
     5041 tcggtggcgc gttgatgggc gcgatgacgg cactgggaat taccgctcgc caccccacgg
     5101 tgaaatgtac cgccagcatg atgggatcgg gctattttac atcgctcgcc cgttcactgt
     5161 ttccaccgct gatacctgaa acggcagcac agcagaatga attcaataaa attgtcgcgc
     5221 cgctggcaga gtgggaagcg acaaaccacc tggaacaact tggggacaga cctctactgc
     5281 tgtggcatgg cctcgacgat gacgttgtgc ctgccgacga atcactacgt ttgcagcagg
     5341 ccttaagcga gacgggacgg gataaactgc taacctgttc atggcagcca ggcgtgcgtc
     5401 accgcattac gcctgaggcg ttagatgctg ccgtgacatt tttccgccag catctttaaa
     5461 cacgcagaat gctgacccct tgcgcttcca gttgttgcag gatttccggg ttagcatttt
     5521 tgccggtgat cagcatatcg atttgatcgg cacggctaaa aagcattccc gcgcgttcgc
     5581 caatcttact gctatcaacc agtaccacca gtttccctac cacgctcagc atcttctgct
     5641 ctgccattgc tgtcagcata tcggttttat acaacccttc tgcggtcagc ccttttccgc
     5701 tggtaaacat ccagtgcccg gcatagagac tgttttcgct gccctgcggg cttaaagtga
     5761 tggactgact tttgttgtac tgtccaccca taatgatcac gctgtcatgt tcttgatcga
     5821 tcaggtaatt tgccagcggt agataattag tgatgatttg cactggcttg ccacacattt
     5881 cccgcccaag cagaaacgcg gtggagccgc agttgatgac tacgctttcg cccggattaa
     5941 ccagctgcga ggccgcttta gcaatacgta ctttttcatc gtgattctgc gcctgatgca
     6001 gattcatcgg cgtccagcgc gggcgctgtt gggtaatagc ttctgcgcca ttgcgcacct
     6061 ttttcagttt gccgctttcg ccaagtttat tgatatcgcg tcgcgcagtg gcaggcgaaa
     6121 ttcccagacg ctcaacgact ttctcaacgg tcacaaagcc caattgtgcg agcatttcta
     6181 ggaggatttg atgtctttgt gcttcagtca tgagctattc cgataaaatt tgattttttt
     6241 agatgatatt tgaaatagcc aggaaatact acgccggata gcgcgaactt ctccacgcta
     6301 tccggtataa acgacagatt acaggaatga cttgaacggc agatccggtt caatagtgaa
     6361 gcaatcatcg aaaccgcgcg gatagtggta ctcgaagttg tctttatcca gcggccaggt
     6421 aaatttgccg ccaacctgcc agataaacgg cttgaagcca tacttcagac gatctttttt
     6481 catctcccac agcacgcgga tctcttgcgg atcggcctgg aagtttgacc agatgtcgtg
     6541 gtggaacggg ataaccactt tcgcattcag ggctttaccc atacgcagca tatcggcgct
     6601 ggtcattttg tcggtaatac cgcgcgggtt ctcaccatac gatcctaacg ccacatcgat
     6661 ctgatgttcg ttaccgtgct tcgcataata gttagagtag tgggaatcgc cactgtgata
     6721 tagggaaccg ccaggggttt tgaacaggta gtttactgcg cgatcgtcca tgccatctgg
     6781 cagtacgcca gccgcttttt gatcggcagg cagggtgatc agtgcagtac ggtcgaaagc
     6841 gtcaagcgca tgaatttcaa tgtctttcac ttttacaaca tcgcccggtt tgaccacgat
     6901 gcaacgctct ttcggtacgc cccagccaat ccacaaatca acgcaggttt tcggtccaat
     6961 gaatggtacg tcatcagcac agttctgcat cacggcagca gcgacgttaa cgtcgatatg
     7021 atcgttgtga tcgtgagtcg ccagtaccgc gtcgatctgg cgaatcgcaa acggatcaag
     7081 aacaaacggg gtggtgcgta ggtttggctg cagttttttc acgccagcca tgcgctgcat
     7141 ctggtgaccc tgtttcatta acgggttacc gtgactttgt ttgccagtgc cgcaccagaa
     7201 atcaacgcaa acgttggtgc caccttctga tttcaaccag atcccggtgc agcctagcca
     7261 ccacatcgca aatgtgccag gggcgacctg ctcttgctca atttcttcat tcagccagct
     7321 accccactcc gggaaagtgc tcaggatcca ggattctcgg gtgatacttt tcactttact
     7381 catcgccatt taccttcatg atagttcaat tcgaatcaat atgtgattgg ttttgattaa
     7441 tcctgacact attttttcag gaaggcaatg accatttttt gactttttgc cagggaagtt
     7501 gttgttgatt ttgagtgtgg aaagatttaa tggaatgtgt agttaaatta actaaatgaa
     7561 tttaaataaa taattgtttc gttgtgtgaa tcccactcta tccgtgtgga attatttgcg
     7621 ggtcgcgcca catttaatca taaataatct tgttgtgatt acttttgaaa attagagtga
     7681 gtgcacaaca ttccgggtgt gcggaatacc cggttacctc ttcttcagga gatcgttatg
     7741 gagatcctct acaacatctt taccgtgttt tttaaccagg tcatgaccaa tgccccgttg
     7801 ttgctgggta ttgtgacctg tctgggctac atcctactgc gcaaaagtgt cagcgttatt
     7861 attaaaggca cgattaaaac cataattggt ttcatgttgt tgcaggcagg gtccggcatc
     7921 ctcaccagca ccttcaaacc ggtggtggca aaaatgtccg aagtctacgg cattaacggc
     7981 gcaatttccg ataattacgc gtcgatgatg gcaaccatcg accgcatggg cgatgcctat
     8041 agctgggtag gttacgcggt gttgttagcg ctggcgctga acatctgtta cgtgctgctg
     8101 cgtcgcatta ccggtattcg taccatcatg ctgactggtc atatcatgtt ccagcaggcg
     8161 gggctgattg ccgttacgtt gtttattttc ggctactcca tgtggacaac cattatctgc
     8221 acggcgattc tggtttcgct ctactggggc attacctcca acatgatgta caagccgact
     8281 caggaagtga cggacggctg cggtttctcc atcggtcacc agcaacagtt tgcatcatgg
     8341 attgcctata aagttgcgcc gttcctcggc aaaaaagagg aaagcgttga agacctcaaa
     8401 ctgccaggct ggctgaatat tttccacgac aacatcgtct ccacggcgat tgtcatgacc
     8461 atcttctttg gtgccattct gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc
     8521 aaagtgcact ggacggtcta tatcctgcaa accggttttt ccttcgcggt ggcgatcttc
     8581 atcatcactc agggtgtgcg catgtttgtg gcggaactct ctgaagcgtt taacggtatc
     8641 tcccagcgcc tgatccctgg tgcggttctg gcgattgact gtgcggctat ttatagcttc
     8701 gcgccgaacg ccgtggtctg gggctttatg tggggcacca tcggtcagct gattgcggtt
     8761 ggcatcctgg tcgcctgcgg ttcctcgatc ctgattatcc caggctttat cccgatgttc
     8821 ttctccaacg ccaccatcgg cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg
     8881 aagatttgtc tggtgatggg gatgatcgaa atctttggtt gcgtctgggc ggtgaaactt
     8941 accggtatga gtgcctggat ggggatggcg gactggtcga ttttggcacc gccgatgatg
     9001 cagggcttct tctccatcgg tatcgccttt atggccgtca tcattgtaat tgcactggct
     9061 tatatgttct tcgctggccg cgcactgcgc gcagaagaag atgcagaaaa acaactggca
     9121 gaacagtctg cttaataagg agttttgatt atgaccgtac gtattctggc tgtgtgtggc
     9181 aacggacaag gcagttccat gatcatgaag atgaaagtgg accagttttt aacccaatca
     9241 aacattgacc acacggtaaa tagctgcgcg gttggcgaat acaaaagcga gttgagcggc
     9301 gcggatatca tcatcgcttc tacgcacatt gcgggcgaaa tcaccgtgac cggcaacaaa
     9361 tacgtggttg gcgtgcgcaa catgctctct cctgccgact ttggcccgaa actgctggaa
     9421 gtgatcaaag cgcatttccc gcaggatgtg aagtaaggac gcgccatgaa attacatgat
     9481 tcgctggcgg agaataaatc catccgcctg caggctgaag ctgagacatg gcaggacgca
     9541 gtaaaaatcg gcgttgacct gctggtggcg gcggatgttg tcgagccgcg ttactaccag
     9601 gcgattctgg atgcggttga acagcacggt ccgtactttg tgctcgcacc aggcctggca
     9661 atgccgcacg gtcgcccgga agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg
     9721 aaaaagccgc tggagttcaa ccacgaagat aacgacccgg tggatatcct catcaccatg
     9781 gcggcggtcg atgccaacac gcaccaggaa gtgggcatca tgcagatcgt caacctgttt
     9841 gaagatgaag aaaatttcga ccgtttacgt gcctgccgta ccgagcagga agtactggat
     9901 ctcattgacc gcaccaacgc ggcagcttaa gaaggaattg aagatgtcat taccgatgtt
     9961 gcaagtcgcg ctggacaacc agactatgga tagcgcctac gaaaccactc gcctgattgc
    10021 cgaagaagtc gacattatcg aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc
    10081 ggttcgcgat ctgaaagcgc tctacccgca caaaatcgtg ctggcagacg ccaaaattgc
    10141 cgatgcgggc aaaattcttt ctcgtatgtg cttcgaagcc aacgctgact gggtgaccgt
    10201 gatttgttgt gcggatatca acaccgccaa aggtgcgctg gacgtagcaa aagagtttaa
    10261 cggcgacgtg cagatcgaac tgaccggtta ctggacctgg gaacaggcgc aacagtggcg
    10321 cgacgcaggt attcagcagg tggtttatca ccgcagccgt gacgcgcagg ccgctggcgt
    10381 ggcgtggggt gaagccgata tcactgcgat caaacgtctt tccgatatgg gcttcaaagt
    10441 caccgccacc ggtggcctgg cgctggaaga tctgccgctg ttcaaaggca ttccaattca
    10501 tgtgtttatc gccggtcgca gcatccgtga tgccgcttct ccggttgaag ccgcgcgtca
    10561 gttcaaacgt tccatcgccg aactgtgggg ctaaggagcg gatatgttgt ccaaacaaat
    10621 cccgcttggc atctatgaaa aagcgctccc cgccggggag tgctggctgg aacgcctgcg
    10681 actggcaaaa acgttgggct tcgattttgt cgagatgtcg gtagatgaaa ctgacgagtg
    10741 cctgtcgcgc ctcgactgga gccgcgagca gcgtctggcg ctggtgaatg cgattgttga
    10801 aaccggcgtg cgtgtgccgt ccatgtgcct ttctgcccat cgtcgtttcc cgttgggcag
    10861 cgaagatgac gcggtgcggg cgcaggggct ggagattatg cgtaaagcca tccagttcgc
    10921 ccaggacgtt ggcattcgcg tgatccagct ggcgggctat gacgtttact atcaggaagc
    10981 caataacgaa acgcgtcgtc gtttccgtga cggcctgaaa gagagcgttg agatggcgag
    11041 ccgcgcgcag gtgacgctgg cgatggagat catggattat ccgttgatga actccatcag
    11101 caaggcgctg ggttacgcgc actatctcaa caacccgtgg ttccagctct acccggatat
    11161 cggcaacctg tcggcgtggg acaacgatgt gcagatggag ttgcaggccg gaatcgggca
    11221 tatcgtcgcg gtgcatgtga aagacaccaa accgggcgtc ttcaaaaacg tgccatttgg
    11281 cgaaggtgtg gtggatttcg aacgttgctt cgaaacgctc aaacagagtg gctattgcgg
    11341 gccgtacctg attgagatgt ggagcgaaac ggcggaagac ccggcggcag aagtggtgaa
    11401 agcgcgcatg gccaaagcag gcatggtgga ggcggcataa tgcaaaagct aaaacagcag
    11461 gtgtttgaag ccaacatgga tctgccgcgc tatgggctgg tgacctttac ctggggcaac
    11521 gtcagcgcta tcgaccgcga acgcgggctg gtggtgatca agcccagcgg cgttgcctac
    11581 gaaaccatga aagcagacga tatggtggtg gtcgatatga gcggcaaggt ggtggaaggg
    11641 gagtatcgcc catcttccga cactgcgacg catctcgaac tctaccgtcg ttacccgtcg
    11701 cttggcggca ttgtccatac ccactccact catgccaccg catgggcgca ggcggggctg
    11761 gcgatcccgg cgttaggcac cacgcacgct gactacttct ttggtgatat accgtgtacg
    11821 cgtggattaa gcgaagaaga ggtgcagggt gagtatgaac tgaacactgg caaagtgatt
    11881 atcgaaacgc tgggcaacgc cgagccgctg catacgccgg gaattgtggt gtatcagcac
    11941 ggaccgttcg cctgggggaa agatgcccac gacgcggtgc ataacgcggt agtgatggaa
    12001 gaagtggcga aaatggcgtg gattgcgcgc agtattaatc cacaactcaa tcatatcgac
    12061 agctacctga tgaataaaca cttcatgcgt aagcacggtc ctaacgctta ttacgggcag
    12121 aagtaaaaca cgcgctgcgg aaatttcctt cctcgggaga taactggtct aattccgcag
    12181 ccgtttttca aaaaaagccc cctgcgaagg gggcaaagca aactatggca atgtttcgtt
    12241 ggttatacct ggtgctagcg ataaatatcc gcgctggcgt gcatattgcc gttactcccc
    12301 ggttcccgca tcagaattac gtggtagtac gttgcgcctt gcgcatctgt ttcttcattt
    12361 aatgcctgac gtgcttcgct ttcagtggcg aaattatgat tgatataaat cacgcctaag
    12421 ctttgcacat cgtccatatt tctggcctgg tggttattaa tttcaatggc tgcccatgta
    12481 tttgcactta gcaaaagcac agccagaagg gctaaaactc gactgaacat agatacctcc
    12541 tcgacggctg actttgtgtg ctctcctctg tgatgatctt ctgatttaat tttaatcaat
    12601 gataaagaag ttgatggtga ccatttctga tgcagttgtt caaaaaaaca ctgtgacgga
    12661 atgtgatgaa cttcaaatca gcggtttaga ggttatttgc gaaaggggag atttatttct
    12721 gctccgccct tgagtttagc ggggtatgtc agtactataa cgcgtcattt ttcagccgac
    12781 ctttaacacg ttccttgcct ccccgggatt cggctgaccc agacaggagg ctgaataatc
    12841 cgtaaggagc aattcgatgc gtcattacga aatcgttttt atggtccatc ctgaccagag
    12901 cgaacaggtt ccgggcatga tcgagcgcta cactgttgcc atcactggtg cagaaggcaa
    12961 gatccaccgt ctggaagact ggggccgccg tcagctggct tacccgatca acaaactgca
    13021 caaagcacac tacgttctga tgaacgttga agctccgcag gaagtgatcg atgagctgga
    13081 aactaccttc cgcttcaacg atgccgttat ccgcagcatg gttatgcgta ccaagcacgc
    13141 tgttaccgaa gcatctccga tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga
    13201 tttcgcaaac gaaaccgctg atgatgctga agctggggat tctgaagagt aatttctgat
    13261 gaccaaccgt ctggtgttgt ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc
    13321 atcaggaatt cctcactgcc agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg
    13381 ctttcaccgg caggcatggt gtcaaatgcc cgttattgtt agcggacacg aaaaccaggc
    13441 cattactcac agtataacgg tcggcagtcg cataaccgtt caggggttca tttcatgcca
    13501 caaggcaaag aacggactga gcaaaatggt tttgcatgcc gagcagattg aattgataga
    13561 ttctggagac tagccatatg gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg
    13621 cggaaggcgt tcaagagatc gactataaag atatcgctac gctgaaaaac tacatcaccg
    13681 aaagcggtaa gattgtccca agccgtatca ccggtacccg tgcaaaatac cagcgtcagc
    13741 tggctcgcgc tatcaaacgc gctcgctacc tgtccctgct gccgtacact gatcgccatc
    13801 agtaatcggt cacggtccat taatacgact ttgagaggat aaggtaatgc aagttattct
    13861 gcttgataaa gtagcaaacc tgggtagcct gggtgatcag gtaaacgtta aagcgggcta
    13921 tgctcgtaac ttcctggtac cgcagggtaa agctgttcca gctaccaaga aaaacattga
    13981 attcttcgaa gtacgtcgcg ctgaactgga agctaaactg gctgaagttc tggcagctgc
    14041 taatgctcgc gctgagaaaa tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg
    14101 cgacgaaggt aaactgttcg gttccatcgg tactcgcgac atcgctgacg ctgtaactgc
    14161 agctggcgtt gaagtggcta agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac
    14221 tggcgaacac gaagtgagct tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa
    14281 cgtagtagct gaataattcg ttattcaacg agacgtaaaa acgccgacca ttggtcggcg
    14341 ttttgctttc tatttttcgt caggcattaa tttcgcaagt agatccattc accattctct
    14401 tgtgtccata agttaggatg gtttgctggc gttagatgca tacgatcact ccttatgtct
    14461 cggtcctgcg gcactcgctc gaaacctaaa cccgagtaga ttgcggctga atttgataag
    14521 gaacgtagac caattttcaa atcatggggt aaatcagaat acgcgtttag caatgcagca
    14581 atgatgaatc cgagggcatt agaaacgcct gtaacagtgc gaagttggtc gatataaaga
    14641 tcgattgttg gtagttcaga tgaccagcaa ttaggcattt gggcaggaag ggttacaacg
    14701 tatcctatcg tttggccggt atagtctgga gtactataac caggtcccaa aacaacctcg
    14761 tgtattgtgt atttactgtt gggttcgaag gattcgacat cttgcaataa atatttcagt
    14821 tctgcggtta acctcgcacc cgcgtaagct ttattgtatt tctgggcgtg aatattatct
    14881 aatgtagctt catggttcca tccaaaagaa ttatcaccat tcattgttac tgaatcgcca
    14941 cgggtttaac agacacctca gagtcattta agatggctta aagagaggtg cccatgagcg
    15001 gtaagcgtta tcccgaagag tttaaaactg aagcagtcaa acaggttgtt gatcgcggtt
    15061 attctgttgc cagcgttgca acacgtctcg atatcaccac ccacagcctt tatgcctgga
    15121 taaagaagta cggtccggat tcttccacta ataaagaaca gtcagatgct caggccgaga
    15181 tccgccgtct ccagaaagag ctgaaacggg ttaccgacga acgggacata ttaaaaaaag
    15241 ccgcggcgta cttcgcaaag ctgtccgact gaggtacg
//
LOCUS       DBKEKI010000088        15094 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.088, whole genome shotgun sequence.
ACCESSION   DBKEKI010000088 DBKEKI010000000
VERSION     DBKEKI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.088"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..123)
                     /locus_tag="ACUQPX_002785"
     CDS             complement(<1..123)
                     /locus_tag="ACUQPX_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HHV9057448.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVP"
     gene            230..526
                     /locus_tag="ACUQPX_002786"
     CDS             230..526
                     /locus_tag="ACUQPX_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HHV9057449.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(660..1085)
                     /gene="arsC"
                     /locus_tag="ACUQPX_002787"
     CDS             complement(660..1085)
                     /gene="arsC"
                     /locus_tag="ACUQPX_002787"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HHV9057450.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1098..2387)
                     /gene="arsB"
                     /locus_tag="ACUQPX_002788"
     CDS             complement(1098..2387)
                     /gene="arsB"
                     /locus_tag="ACUQPX_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HHV9057451.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2441..2794)
                     /gene="arsR"
                     /locus_tag="ACUQPX_002789"
     CDS             complement(2441..2794)
                     /gene="arsR"
                     /locus_tag="ACUQPX_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HHV9057452.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            3537..3620
                     /gene="dinQ"
                     /locus_tag="ACUQPX_002790"
     CDS             3537..3620
                     /gene="dinQ"
                     /locus_tag="ACUQPX_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HHV9057453.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3674..5026)
                     /gene="gorA"
                     /locus_tag="ACUQPX_002791"
     CDS             complement(3674..5026)
                     /gene="gorA"
                     /locus_tag="ACUQPX_002791"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HHV9057454.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEDPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5098..5940)
                     /gene="rlmJ"
                     /locus_tag="ACUQPX_002792"
     CDS             complement(5098..5940)
                     /gene="rlmJ"
                     /locus_tag="ACUQPX_002792"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HHV9057455.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6143..8185
                     /gene="prlC"
                     /locus_tag="ACUQPX_002793"
     CDS             6143..8185
                     /gene="prlC"
                     /locus_tag="ACUQPX_002793"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HHV9057456.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8193..8945
                     /gene="rsmJ"
                     /locus_tag="ACUQPX_002794"
     CDS             8193..8945
                     /gene="rsmJ"
                     /locus_tag="ACUQPX_002794"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HHV9057457.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8994..10463)
                     /gene="dtpB"
                     /locus_tag="ACUQPX_002795"
     CDS             complement(8994..10463)
                     /gene="dtpB"
                     /locus_tag="ACUQPX_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HHV9057458.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQSVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10629..10682
                     /locus_tag="ACUQPX_002796"
     CDS             10629..10682
                     /locus_tag="ACUQPX_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057459.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(10781..11215)
                     /gene="uspA"
                     /locus_tag="ACUQPX_002797"
     CDS             complement(10781..11215)
                     /gene="uspA"
                     /locus_tag="ACUQPX_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HHV9057460.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11606..11941
                     /gene="uspB"
                     /locus_tag="ACUQPX_002798"
     CDS             11606..11941
                     /gene="uspB"
                     /locus_tag="ACUQPX_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HHV9057461.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12012..13511)
                     /gene="pitA"
                     /locus_tag="ACUQPX_002799"
     CDS             complement(12012..13511)
                     /gene="pitA"
                     /locus_tag="ACUQPX_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HHV9057462.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13743..14942
                     /locus_tag="ACUQPX_002800"
     CDS             13743..14942
                     /locus_tag="ACUQPX_002800"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HHV9057463.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKIPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3766 a         3868 c         3991 g         3469 t
ORIGIN      
        1 tggcaccttt tgtcttatca ggctgacacc ggcactaatc actataaaca gcaacgaaag
       61 ctcgacggcg agaaacacaa acatctctgc ggcatcttgc agcatcgcaa gccaactact
      121 catacgtaac ctcattggat aaatctatat ttccagaata atcgaaataa aattcagatc
      181 aagatatttc tggtattatc gaaatatcaa ccagacagag gggtgtcata tgcagttgga
      241 agaagtcgct aaagcgctga aagagctagg tcacccgacc cgtttgttca tatttaaaca
      301 tcttgtcaaa gccggggaac aagggttgcc ggtcggtgag ttgcaaaagc agcttgggat
      361 acctggctcc acactcagcc accatatatc agcactggta tcggtaggac tggtcaaaca
      421 aaaccgtgag agtcggacgc tgatgtgtgt ttcgcaatac gcaatgctgg aagcgataat
      481 tgaattctta cgcgaggagt gctgcgtaaa tagcaaaatt gcctgatgcg ctacgctcta
      541 catatttcat gccatatatt gaatttgcac ggttttgtag gtcggataag acgcgttagc
      601 gtcgcatccg acataaacaa agcgcacttt accaactatc ggcagtggat atcgccgctt
      661 tatttcaggc gcttacccgc ttcatcaacc actttctcgc catcttcctt ggagaatgcg
      721 cctttttgcg catctggcag aatttccagc accacttctg aagggcggca caggcgagtt
      781 cccagcggcg tcaccacaat cgggcgatta atcagaatcg ggtgctgaag cataaagtcg
      841 attaaccgat cgtcagtaaa tttatcttcc gcaaggccca gctcctcata cggttcgacg
      901 tttttacgca gcagcgcgcg tacggaaatc cccatatcgg caatgagttt gaccagttca
      961 tcgcgcgttg gcggagtttc cagataatgg ataatggtcg gttctgtgcc gctgttgcgg
     1021 atcatctcca gcgtattacg cgacgtgccg caggccgggt tgtgataaat ggtaatgttg
     1081 ctcatatcag tatctcatta caaagtgaaa gagagacgta gcgccagcgc agccagcgtc
     1141 acaaacagca caggcagggt catgataatc cctgtacgga agtaatatcc ccagctgata
     1201 gtcatattct tctgcgaaag tacgtgcagc cagagcagcg ttgccaggct accaatcggg
     1261 gtaattttcg gccccaaatc gcagccaatc acgttggcat aaaccatcgc ttctttgata
     1321 acgccagatg ccgtgctgcc atcaatggac aacgcgccaa ccagtaccgt cggcatattg
     1381 ttcataatag aagagaggaa ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg
     1441 ccgttatccg ccagcacgtt gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc
     1501 aggccataaa ccaccagata catgccgagc gagaagatga caatctgcca gggggcaccg
     1561 cgcaggactt tacccgtatt aatcgcatga ccgcgtttag cgacgacaaa taatatcagt
     1621 gcgcccacag ctgcaatggc gctcaccgga atgccgagcg gttccaggac gaaaaatccc
     1681 accagcagaa gcagtaaaac aacccagcca gttttgaacg tagcaggatc tttgatcgct
     1741 tctgcgggag atttcagcag cgccatatcg tagttctgcg gaatatcttt gcgaaaatag
     1801 agatgtagca tcaccagcgt ggcaacaatc gcggcgatat ccaccggcac catcaccgag
     1861 gcgtattcgc gaaagccgag gccaaagaaa tcagcggaaa cgatattcac caggttggag
     1921 acaataagcg gcaggctggc ggtatcggca atgaatccgg ccgccatcac gaacgccagc
     1981 gtcgtgcctt tactgaatcc taaagccagc agcatggcga tgacaatcgg cgtcaaaata
     2041 agcgccgcgc catcattggc aaacagggcc gcaacggcgg caccgagcag gacaatccag
     2101 gtaaacagca agcgaccgcg tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca
     2161 aaaaagccgg actcatccag cagcaggctg atgataatga cggcaataaa cgccgccgtc
     2221 gcgttccaga cgatattcca caccaccgga atatcacccg gatgaaccac gcccgtaacc
     2281 aacgccagta ccgcgccgag cgttgcactc cagccgatgc ctaaaccttt cggctgccag
     2341 ataaccaata cgatggtcag gacaaagata gcgcctgcca gtaacataat gcctcccgga
     2401 taaaacacat gtgcaaatcc atatgtgttt agctaaattt ttaactgcaa atgttcttac
     2461 tgtctccgga acagttttgt ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt
     2521 cacatcgcca ggcttcatca ataattttcg ccgcccatgc tggaatatgc ggcgataagc
     2581 ggtaatgaac ccacttacct tgcttgcggt ccagcaatag cccgctttca cgcagcaatg
     2641 ccagatggcg ggatatcttg ggctgcgact gatcgagggc agtgcagaga tcgcagacgc
     2701 ataactctcc cagttcgcta agcagtaaaa cgatgcccag acgggtttca tcagcaagaa
     2761 ttttgaacaa ttggatgggt aacagaaatg acatattgcg ctcctgattg ttgcaggtag
     2821 tgtctctctt cgaagcggat aagtcaaaaa catatatgac ttaacgaatg tgtaagtgca
     2881 gaggaaggta ataggtgtga attttgagtt ggctattcat ttgaaaggcg gtttgaatca
     2941 ggacgtaaaa aaaccgactt tgcgtcggtt ttttactctt cagcctgagt tggtggctct
     3001 ggctggagtg agaatgccca catctaacgg ttcaatacta ttagccagtg gctaacattg
     3061 caagcgttag cgatttcttc agctggagaa gggacagttt gctatacatg gtggtgaaag
     3121 aggatttata tactttcatc gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta
     3181 aaaaaaccga cttcgcgtcg gtttttttac tcttcagccc agagttggtg gctctggctg
     3241 gagtgagaat gccatcatct aacgcatcaa tgctaatacc atactgaaac tattgcaagg
     3301 ttatgctggt tatttagcct aattacactg tatgattatc cagttagctc tgaggcattt
     3361 tcactctggc aatgcgcata aacgctttca aagtcctggt cagaagtacg ggtggtgccg
     3421 ttaactgatg ctctggccgg agtgagattg cccacatcta acaatgaggc atgcgccgtg
     3481 acaggcagtg gatgagtaag cggatgcatt ctcactccat cgcatggaga aaacgggtga
     3541 ttgataaagc aatcatcgtt cttggggcgt taattgcgct gctggaactg atccgctttc
     3601 tgcttcagct tctgaactga tagcggaaac gtaattaagg gctaagagca cactactctt
     3661 agccctttaa catttaacgc atggtcacga actcttctgc cgccgttggg tgaatggcga
     3721 cggtattgtc gaagtctttt ttggttgcgc ccatcttcag cgccaccgcg aagccctgca
     3781 acatttcgtc cataccaaag ccaatgccgt gaataccgac aatcttctct tctggcccaa
     3841 cgcacaccag cttcatgcgg cacggctggc ggtgagtggt gacggcggta tacatcgcgg
     3901 tgaaagagga tttatacact ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt
     3961 ccgttaaacc aacagtacca atcggcggat ggctgaagac cacggtcgga atgttgctgt
     4021 aatccagatg ctcatccggc ttgttattaa acaggcgttc agagagacgg cgacccgctg
     4081 caactgccac cggtgtcagt tccactgcac ccgtgttatc gcccaccgcg taaatacctt
     4141 caatattggt gttttgatat ttatcgacga cgatatagcc tttttcgttg gttttaacgc
     4201 cagcggcttc gaggttgatg ttgtcattgg caggctcgcg accaatcgcc caaatcaggc
     4261 aatccaccgt ttcactgcga ccatcttcca gctccagcgt caggctacca tcggcatttt
     4321 tcactaccgc tttcgggatg gcgttggtat gcaactgtgg gtcttcggcg ttcatcactt
     4381 cgaccagcgt ttcagagatc atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa
     4441 acagatgcgt tttcgcgccg aggccgttaa tcacgccagc cagctcaacg gcgatgtaac
     4501 ccgcgccaac aaccgccacg cgctctggca aagcaggaag ggcgaagaag ccatcggaat
     4561 caataccgta ttccacgcct ggaatatccg ggtggctcgg acgaccgccg gtggcgatca
     4621 gaatatgatc ggccgtgatg gtttcgccgt taacttccag cgttttggca tcaacgaagc
     4681 gggcaaagcc tttgattaca tcaacgttat ttttaccgag cacgttttca taggaagtat
     4741 gaatacggtc gatataggcg gtacggctgg cgatcaacgt ttcccagttg aacttgttga
     4801 tggtggtatc gaaaccataa tcagggccgt acatatggat cgcttcacgg atttgcgccg
     4861 cgtgccacat cacttttttc ggcacgcagc cgacgttcac gcaggtgccg cccagctctt
     4921 tggcttcaat cagtgcgcat ttctggccgt acatagccgc gcggttgatg gaggcgatac
     4981 cgccgctgcc gccgccgatg gcgatgtaat catagtgttt agtcatgaca tattgtcctt
     5041 atcgttgatt accgcgattg tagcgcgaga cgtaataggt gccagcaatg gctgcaatta
     5101 ctccggcacg atccagctta cggtggcgtg cccggtacct gccggaacca gtttgctgtg
     5161 cagccacgac agcacgttat tcatctgctg ttccagcttc cacggcgggt taatcacaat
     5221 catgccggaa gcggtcatgc cacggcgatc gctgtctggc agcaccgcca gttcaatttg
     5281 cagaattttg cgaatgccgg tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg
     5341 cagcaccacc ggataccaca atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc
     5401 tatcccgctg accaccgcct gatagtcggt tttcatttca tacggcgggt cgataaggat
     5461 taaaccacgg cgagaaaccg gcggcagttt ggccttaagt tgctggaaac cgtcggcttt
     5521 ttcgacgcgt gcacggctgt ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg
     5581 gtgcagttcg gtcagttgca ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg
     5641 cgaaccgggg tagtaacgca actggccgct acggttgaag tgttttacca cattgatgta
     5701 cgcctccagt tctgcgggca aatcgtcctg ctgccagata cgggcgatac cttcgagata
     5761 ttcgccggta cgctcggcat gttcgctacc taactgataa cgcccggccc ctgcgtgggt
     5821 gtcgagatag agaaacggtt tatctttctc tttcagcgac tcgatgatca ggctctgaac
     5881 ggtatgttta aggacgtcgg cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat
     5941 gggtaaaggt gttccggtaa gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc
     6001 aggacagtat accgaaaaga ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc
     6061 actacactta accccatgct acacacatta tgtaaagcgc ctgttgagcg cttccttaac
     6121 ctctttaacc aggactgcgc taatgacgaa tccgttactg actccctttg aattgcctcc
     6181 gttttctaaa attctcccgg aacatgtcgt tccagccgtg actaaggcat tgaacgactg
     6241 ccgcgaaaat gtggagcgcg tagtagcgca aggggcaccg tacacctggg aaaatctctg
     6301 ccagccgttg gcggaagtgg acgatgtttt ggggcgtatc ttctccccgg tcagccacct
     6361 gaactcggtg aaaaatagcc cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct
     6421 gtcggaatac agcacctggg tagggcaaca tgaagggctg tataaagcgt accgcgacct
     6481 gcgcgatggc gatcattacg ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc
     6541 cctgcgtgat tttgaactct ctggcatagg tctgccgaaa gagaaacagc agcgttacgg
     6601 cgaaattgct acccgtcttt ctgaactggg caaccagtac agcaacaacg tcctcgatgc
     6661 gacgatgggc tggaccaaac tcgttaccga cgaagcgggg ctggcgggga tgccagaaag
     6721 cgcgctggct gcggcaaaag cccaggccga agcgaaagag ctggaaggct acctgctgac
     6781 gctggatatc ccaagctacc tgccggtaat gacctactgc gacaaccagg ctctgcgcga
     6841 agagatgtat cgcgcttaca gcacccgcgc ctccgatcaa ggcccgaacg ccggtaagtg
     6901 ggataacagc aaggtgatgg aagagatcct cgcgctgcgt cacgaactgg cgcaactgct
     6961 gggctttgaa aactacgcct ttaaatccct tgccactaaa atggcagaaa acccgcagca
     7021 ggtgctggat ttcttaaccg atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct
     7081 ggagcaactg cgtgccttcg ctaaagccga atttggtgtc gatgagttgc agccgtggga
     7141 tatcgcttac tacagcgaaa aacagaaaca gcacctctac agcatcagcg acgagcagct
     7201 gcgtccgtac ttcccggaaa acaaagcggt taacggcctg tttgaagtgg tgaaacgtat
     7261 ttacggcatc accgctaaag agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt
     7321 cttcgaactg tatgacgaaa ataacgaact gcgcggcagc ttctacctcg atctgtatgc
     7381 ccgtgaaaac aagcgcggcg gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc
     7441 tgatggttct ctgcaaaaac cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa
     7501 tggtaaaccg gcgctgttca ctcacgacga agtgatcacc ctgttccacg agttcggtca
     7561 cggcctgcac catatgctga cccgcatcga aaccgctggt gtttccggta tcagcggtgt
     7621 gccgtgggat gcggtcgaac tgccgagtca gtttatggaa aactggtgct gggagccgga
     7681 ggcgctggcg tttatctctg gtcactatga aaccggcgaa ccgctgccga aagagttgct
     7741 ggataaaatg ctggcggcga agaactacca ggcggcgctg tttattctgc gtcagctgga
     7801 gttcggcctg tttgatttcc gccttcatgc cgagttccgc ccggatcagg gggcaaaaat
     7861 cctcgaaact ctggcagaaa tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg
     7921 ccgtttcccg cacgctttca gccatatttt cgccggtggt tatgcggcag gttactacag
     7981 ctacctgtgg gctgacgtac tggcggcaga tgctttctcg cgctttgagg aagagggcat
     8041 tttcaaccgt gaaaccgggc agtcgttcct cgacaacatt ctgagccgtg gcggttcaga
     8101 agagccgatg gatctgttca aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct
     8161 ggagcattac ggcattaagg gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag
     8221 gcgccggaga cggcgcctta tctgttctgg cggcccgctg ggggctggag cacgatgaag
     8281 acaacctgat ggcgctggtg ttaacgccgg aacatctgga attgcgcaag cgtgatgagc
     8341 caaaacttgg cggcatcttt gttgattttg ttggcggagc gatggcgcac cgacgcaaat
     8401 tcggcggtgg tcgcggtgag gcggtggcga aagcggtggg cattaaaggc gattatttgc
     8461 cggatgtggt ggacgccacc gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg
     8521 gctgccgcgt gcggatgctg gagcgtaatc cagtggttgc cgcgctactc gacgacggcc
     8581 tggcgcgtgg ttatgcggat gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa
     8641 ttcacgcctc cagcctgacg gcgctaaccg atattacacc acgcccgcag gtggtctatc
     8701 tcgatccgat gttcccgcat aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct
     8761 ttcagtcgct ggtggggccg gatcttgatg ccgatggatt actggagcct gcacgcttgc
     8821 tggcgaccaa acgcgtggtg gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg
     8881 ccacgccaaa cgcggttgtg actaaagggc atcgctttga tatctatgca ggtacgccag
     8941 tgtaaaagta aagcagggta gcgataacgc taccctgctt tctgcaagaa taattaatgg
     9001 ctttccggcg tcgcaatcat gcgtttcagc cacggcacca tcaacagcat cactactgca
     9061 acgcccagcg tgaccagacc aatcttacca aacacgttgg tatagacggg caacgtctca
     9121 agcggatcgg taatgttgtc cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag
     9181 aacgcggcag cctgcgtcag gaaccacatc ccgagaataa agcccatcaa atgctgcggc
     9241 accagggcag caatcatcgc caggccaagg gcgctaataa acaattcacc caggctctgg
     9301 aataaataca ccagcacgat aaaccatggc gatgtcagcc cctgggcatc cgcaaaccac
     9361 attcccgcag cagcagccgt caaaaagccc agtgagcaca taaacatgcc gagagtaaat
     9421 ttcatcggca tcgagaggtc tttgcctttg ttacccagat gcgtgtaaat gcctgccagt
     9481 attgggctgg cgagtaccac ccagaacggg ttaagcgcct ggaagctgac cgggttgatg
     9541 gaaaaaccga gaatttcatg atgcacgttg ttgatggcaa agaagttcag cgatgttggc
     9601 atctgggcgt agagaatgta aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac
     9661 attttattgc gcccggtttt atccagcttg aatgcctgac gaaagaagat gatggtgacg
     9721 acgatggaga gaacaatcag caccagattg gcgacttcta cgttgtgcat cagccatgca
     9781 catacgaaga tcatcaccac gctgccaagt aatacgtaca acagtttgct gaagctcatc
     9841 ggcttgaagt cgggttcaga accaatgtct ttcaccattc cacgacaggc gatgtaaacc
     9901 agtaatgcga taattaaccc cgccccgcac aggttgtagg tgactgaata accgaatcta
     9961 tcagcgatca caggggccag cgataacgct atcaacgagc cgatattgat cgacatatag
    10021 aacagggtga atgcgccatc aagccgcgga tctttcggcg gatagcactt cgaaagcaag
    10081 ctggctgggt tagctttaaa caggccgtta ccgacagcga tagtccctag ggcgatgaaa
    10141 atcaggtcag gcttaagtag cgacatgccg gtcatgaagt agccaatcgc cagcacaagt
    10201 gctccaagaa caatggtgcg tttggtcccc aacaggtggt cgccgacata gccgccaatg
    10261 gaaatgaggc catagaccag tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc
    10321 gagaatccaa gctgtttaac gaagaaaacc gccagtacgc tctgcacgcc gtagtagccg
    10381 aatcgctccc ataactcgac aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc
    10441 cccatgggtg ttgttgtatt aatatttatt aaccttccaa taccatcctg aaaagtatga
    10501 acgcgtaagg gataacgcgc gaaaaagttt acatctgtca gagattctta atattgatgc
    10561 ggtgaataga ttaatacact attacaacag aaaataacca gctgatcaca ctaatgatgg
    10621 aaaatatgat ggtgttaaat tttgtattgg caggagattt tgattgcgta cgcagatcgt
    10681 aggccagata aggggtttac gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc
    10741 cgcatatgcg ggcgttcagg aacacgtcgt ggagggatga ttattcttct tcgtcgcgca
    10801 gcggaacaat cagcatatca acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca
    10861 gtttgctcca gaagtcctgg tggtgaccac aaaccaccag atccatatcg tatttcttga
    10921 ttgcatcgac cagaacctgg cccaggtcgc cgctgccgct cagggtttca gtgattggat
    10981 agcctgcatt agtggaaagc tcggtcagcg catgatgtgt ctcttcagag atgcgtttct
    11041 gcatatcacc cagattcaca tcaataagcc cggtgtatag gtcagagtag tttacatcta
    11101 cgtggatcag agaaactttc gcattgtagg ggcgagccat agagactgct ttctctacca
    11161 gaactttgct ttccggggag agatcgactg cgatgagaat gtgtttataa gccatagtgt
    11221 tactccttcc ataaagttgt cgatgactgg ccagctagcg tttcttgtgc cgttacgtca
    11281 cccgcgtcct gcgaactacg tgcgcggggc taacccagcc gtaaagattc agtgttaaga
    11341 ccatccttac cttatagcga cccggatgat ccgtcaatcc gccttgctta cccgttagat
    11401 aaacaaattt tcgacatgaa tatattgata gtggttaacc ttctggaaaa aaaacaacct
    11461 gatctcctac actatctata gagccgctcg gatgttggtc accacaaaag cggtttctcc
    11521 aggacctggt gttaactgat tggcagcgta tcgggagcgg tagccccggg gtggaactcc
    11581 gcgggcaggt cgccggggag gaggtatgat aagcaccgtc gcattatttt gggctttatg
    11641 tgtcgtttgc attgttaaca tggcgcgcta tttctcatca ctacgcgcgt tgttagtggt
    11701 actgcgtaac tgcgatccat tgctctatca atatgttgat ggagggggct tttttacctc
    11761 acatggccaa cccaacaaac aggtgcgtct cgtttggtat atctatgccc aacgttatcg
    11821 cgatcatcac gatgatgagt ttattcgccg ctgtgagcgg gtgcgtcggc agtttattct
    11881 gactagcgca ttgtgtggtc tggtggtggt cagcctgatt gcattgatga tttggcatta
    11941 agcacttcag caataataac gcataaaaaa agcgggccag taaactgacc cgctcgtttt
    12001 ggtgcgtacg attacaggaa ctgcaaggag agccagtaca gcccgccgga aagtagtacc
    12061 gcagccggaa gggtaaacac ccaggccatc agaatactgg ttacggtttt acgctgtaag
    12121 ccgccgccat ctaccaccat cgtccccgcg acagaagagg agagtacgtg agtggtggaa
    12181 accggcatcc cggtataact cgccaggccg attgacactg ccgccgtcat ctgggcagac
    12241 atcccctgag cgtaggtcat gcctttctta ccgattttct caccgatagt cgttgccaca
    12301 cgacgccagc caatcatcgt accgatacct aacgccagcg cgaccgccat gatgatccac
    12361 accggcgcat actcgatggt gctaagcatg tcggacttca gtttcttcaa caggcgctga
    12421 tcgtcagcac tcacaccagg catcttcacc actttgtcga tagtgtcaga aacgcacagc
    12481 ataatgcggc gcatctggct acgttgatca agcgacagct tgtcgtagct ttccacatcg
    12541 gtggtcaaca tacctttcag gcggttgagc gcgttaatgg tattcgacgg atggcagtgg
    12601 aactccgcag gttgcgttgc gccagcttcc ggagccggta ctaactgatc agcaccggta
    12661 gcctgtttga gcagcgcagg atgctgctca aagtaagctt cgacgttgtt gatggcatca
    12721 cgggtacggg tgatttcgta gccagtggca ttcatgttca ccacgaagcc tgctggcgcg
    12781 acgccaatca ataccaacat aaccagacca atgcctttct gaccatcgtt cgcgccgtgc
    12841 gaaaacgcca cgccgatagc ggaaaggatc agcgcgatac gcgtccagaa cggcggcttt
    12901 ttcttgccgt ctttcttttc acgctccgct ggggtcaggt ggatacgggc gcgtttcttg
    12961 gtgccgctcc agtagcgacg cagcaagaaa atcagaccgc cagcaaacac caggccgaca
    13021 ataggggaaa cgatcagaga accgaaaata cttaatactt tcgggatatt gagtgcatcc
    13081 accactgacg tcccggtcat caacgcattg gttaaaccaa tcccgatgat cgcgccaatc
    13141 agcgtatgag agctggatgc aggtaaacca aagtaccagg tacccaggtt ccagataatc
    13201 gccgccagca acatagagaa caccatggca aggccatgag acgatcccat attaagcagc
    13261 agatccgtcg gcagcatatg cacaatggca taggcaacac tcagaccacc cagcaaaaca
    13321 cccaaaaagt tgaacaccgc cgccataacc acggcgagct gagaacgcat cgcgcgggta
    13381 tagataacgg ttgccacggc gttggctgtg tcatggaaac cattgatggc ttcgtagaac
    13441 agcacaaaag ccagtgcaag caataataac agcccggtat gcaaatccag gccagcaaac
    13501 aaatgtagca taggacgtta cgccattttg aggacatgaa cgcggcgcat tatcagtgac
    13561 tttcgcggcg cgggcaaagt gaaatataga ctttttttga tttgcctcct gtatggattt
    13621 cactcaaaaa ataattatct tatataattc aggcaaatac ttccttttag taatattgat
    13681 gctggtgcga ccactgagga atctttacaa ttcacgcccg ttttttctaa gaggagcgca
    13741 acgtggaaag gtttgatgcc attattatag gcgcgggtgc ggcgggtatg ttctgttctg
    13801 cgctggcagg tcaggcagga cgccgggttc tgctgatcga taatggtaaa ataccagggc
    13861 gcaaaatcct tatgtctggc ggtgggcgct gcaactttac caacctttat gtcgaaccag
    13921 gcgcttatct gagccagaat ccgcattttt gtaagtctgc actcgcacgt tttacccagt
    13981 gggatttcat tgatctggtc aataaacacg gtatcgcctg gcacgagaaa acgttagggc
    14041 aactcttctg cgatgactcc gcgcagcaga ttgtcgacat gctggtggat gagtgcgaga
    14101 agggcaatgt aaccttcaga ttgcgtagcg aagtgctgag tgtggcgaag gatgaaacag
    14161 gcttcacgct tgatctgaac ggcatgactg tcggttgcga aaagctggtc atcgcgaccg
    14221 gtgggctgtc aatgccgggg ctgggcgcat cgccgtttgg ttataagatt gccgaacaat
    14281 ttggcctcaa cgtgctgccg acccgcgcgg gtctggtgcc attcactctg cataaaccgt
    14341 tgctcgaaga gttacaggtg ctggcgggcg tggcggtgcc ttccgtgatt accgctgaaa
    14401 acggcaccgt tttccgtgag aacttactct tcacccaccg cggcttatct ggaccggcgg
    14461 tgttgcagat ttcaagctac tggcaaccgg gtgaatttgt cagtatcaat ctgctaccgg
    14521 atgtggacct cgaaaccttc ctgaatgagc agcgtaacgc acatccgaat caaagcctga
    14581 aaaacacact ggcggttcat ctaccgaagc ggttggttga acgcttacag caactcgggc
    14641 aaatcccgga tgtttcgcta aaacagctca acgtgtgtga ccaacaggca ctgattagca
    14701 cattgaccga ctggcgcgta caacccaacg gcactgaagg ctatcgcact gccgaagtga
    14761 cgctcggcgg cgtggacacc aacgaacttt cttcacggac gatggaagcg cgcaaagtgc
    14821 ctgggctgta cttcatcggc gaagtgatgg acgttaccgg ctggctgggg ggctataact
    14881 tccagtgggc gtggtcgagt gcatgggctt gtgcgcagga tttggcgtca attgtgaaat
    14941 aaccatgacg ttttcccgtc agccgtttgt atatgtcgtt attaagtcat tttggtacgg
    15001 ttctgcatcc gacattctgg tgttaactcg ctgttttgtt agcgaattaa cgctattgcc
    15061 gttgtggtta ttttatagtg tatttattct caga
//
LOCUS       DBKEKI010000089        15031 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.089, whole genome shotgun sequence.
ACCESSION   DBKEKI010000089 DBKEKI010000000
VERSION     DBKEKI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.089"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..733
                     /locus_tag="ACUQPX_002801"
                     /pseudo
     CDS             1..733
                     /locus_tag="ACUQPX_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
     gene            770..2518
                     /gene="msbA"
                     /locus_tag="ACUQPX_002802"
     CDS             770..2518
                     /gene="msbA"
                     /locus_tag="ACUQPX_002802"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HHV9057464.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            2515..3501
                     /gene="lpxK"
                     /locus_tag="ACUQPX_002803"
     CDS             2515..3501
                     /gene="lpxK"
                     /locus_tag="ACUQPX_002803"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HHV9057465.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            3538..4771
                     /gene="ycaQ"
                     /locus_tag="ACUQPX_002804"
                     /pseudo
     CDS             3538..4771
                     /gene="ycaQ"
                     /locus_tag="ACUQPX_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
     gene            4823..5005
                     /gene="ycaR"
                     /locus_tag="ACUQPX_002805"
     CDS             4823..5005
                     /gene="ycaR"
                     /locus_tag="ACUQPX_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HHV9057466.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            5002..5748
                     /gene="kdsB"
                     /locus_tag="ACUQPX_002806"
     CDS             5002..5748
                     /gene="kdsB"
                     /locus_tag="ACUQPX_002806"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HHV9057467.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            5902..6795
                     /locus_tag="ACUQPX_002807"
     CDS             5902..6795
                     /locus_tag="ACUQPX_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HHV9057468.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(6772..7551)
                     /gene="elyC"
                     /locus_tag="ACUQPX_002808"
     CDS             complement(6772..7551)
                     /gene="elyC"
                     /locus_tag="ACUQPX_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HHV9057469.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            7687..8472
                     /gene="cmoM"
                     /locus_tag="ACUQPX_002809"
     CDS             7687..8472
                     /gene="cmoM"
                     /locus_tag="ACUQPX_002809"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HHV9057470.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRLGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            8469..9791
                     /gene="mukF"
                     /locus_tag="ACUQPX_002810"
     CDS             8469..9791
                     /gene="mukF"
                     /locus_tag="ACUQPX_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HHV9057471.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMKLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            9772..10476
                     /gene="mukE"
                     /locus_tag="ACUQPX_002811"
     CDS             9772..10476
                     /gene="mukE"
                     /locus_tag="ACUQPX_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HHV9057472.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            10476..14936
                     /gene="mukB"
                     /locus_tag="ACUQPX_002812"
     CDS             10476..14936
                     /gene="mukB"
                     /locus_tag="ACUQPX_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HHV9057473.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADRGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
BASE COUNT         3572 a         3695 c         4229 g         3535 t
ORIGIN      
        1 aatggtgata gccagaaacg gcaaagcgat tctctatgac acaggactgg cctagcctga
       61 aggggatagc gggcaacaac tgattatccc ttggctccac tggcataatc ttgaaccgga
      121 aggcgttatt ctgagccatg aacatctgga tcaccgggga gggctggatt caatattgca
      181 tatatggccg atgttatgga tcagaagtcc gttaaactgg gaacatcatc agccctgtgt
      241 gcgtggcgaa gcgtggcagt ggcaaggatt gcgtttcagc gcgcactggc ctttacaagg
      301 tagcaacgat aagggaaata accattcctg tgtggttaag gttgatgacg ggacgaatag
      361 cattcttcta accggtgata ttgaagcccc agctgaacaa aagatgctaa gccgttactg
      421 gcagcaagtg caggcaacat tgcttcaggt acctcaccat ggcagtaata cctcatcatc
      481 gttgccatta attcagcgag tgaatggaaa agtggcactc gcatcggcat cgcgctataa
      541 cgcatggcga ttgccctcta acaaagttaa gcatcgctat caacagaaag gatatcaatg
      601 gcttgatact ccacatcagg gtcaagtgac ggtcaatttt tcagcacaag gctggcggat
      661 tagcagcctc agagagcaaa ttttacctcg ttggtatcat cagtggtttg gcgtgccagt
      721 ggataacggg tagaatatgc ggctatttca acaaatgctg gttttttgaa tgcataacga
      781 caaagatctc tctacgtggc agacattccg ccgactgtgg ccaaccattg cgcctttcaa
      841 agcgggtctg atcgtggcgg gcgtagcgtt aatcctcaac gcagccagcg ataccttcat
      901 gttatcgctc cttaagccac ttcttgatga tggctttggt aaaacagatc gctccgtgct
      961 ggtgtggatg ccgctggtgg tgatcggact gatgatttta cgtggtatca ccagctatgt
     1021 ctccagctac tgtatctcct gggtatcagg aaaggtggta atgaccatgc gtcgccgcct
     1081 gtttggtcac atgatgggaa tgccagtttc attctttgac aaacagtcaa cgggtacgct
     1141 gttgtcacgt atcacctacg attccgaaca ggttgcttct tcttcttccg gcgcactgat
     1201 tactgttgtg cgtgaaggtg cgtcgatcat cggcctgttc atcatgatgt tctattacag
     1261 ttggcaactg tcgatcattt tgattgtgct ggcaccgatt gtttcgattg cgattcgcgt
     1321 agtatcgaag cgttttcgca acatcagtaa aaacatgcag aacaccatgg ggcaagtgac
     1381 caccagtgca gaacaaatgc tgaagggcca caaagaagta ttgattttcg gtggtcagga
     1441 agtggaaacg aaacgcttcg ataaagtcag taaccgaatg cgtcttcagg ggatgaaaat
     1501 ggtttcagcc tcttccatct ctgatccgat cattcagctg atcgcctctt tggcgctggc
     1561 gtttgttctg tatgcggcga gcttcccaag tgttatggat aacctgactg ccggtacgat
     1621 taccgttgtt ttctcttcaa tgattgcact gatgcgtccg ctgaaatcgc tgaccaacgt
     1681 taacgcccag ttccagcgcg gtatggcggc ttgtcagacg ctgtttacca ttctggacag
     1741 tgagcaggag aaagacgaag gtaagcgcgt gatcgagcgt gcgactggcg acgtggaatt
     1801 ccgcaatgtc acctttactt atccgggacg tgacgtaccc gcattgcgta acatcaacct
     1861 gaaaattccg gcagggaaga cggttgctct ggttggacgc tctggttcag gtaaatcaac
     1921 catcgccagc ctgatcacgc gtttttacga tattgatgaa ggcgaaatcc tgatggatgg
     1981 tcacgatctg cgcgagtata ccctggcgtc gttacgtaac caggttgctc tggtgtcgca
     2041 gaatgtccat ctgtttaacg atacggttgc taacaacatt gcttacgcac ggactgaaca
     2101 gtacagccgt gagcaaattg aagaagcggc gcgtatggcc tacgccatgg acttcatcaa
     2161 taagatggat aacggtctcg atacagtgat tggtgaaaac ggcgtgctgc tctctggcgg
     2221 tcagcgtcag cgtattgcta tcgctcgagc cttgctgcgt gatagcccga tactgattct
     2281 ggatgaagct acctcggctc tggataccga atccgaacgt gcgattcagg cggcactgga
     2341 tgagttgcag aaaaaccgta cctctctggt gattgcccac cgcttgtcta ccattgaaaa
     2401 ggcagacgaa atcgtggtcg tcgaggatgg tgtcattgtg gaacgcggta cgcataacga
     2461 tttgcttgag caccgcggcg tttacgcgca acttcacaaa atgcagtttg gccaatgatc
     2521 gaaaaaatct ggtctggtga atcccctttg tggcggctat tgctgccact ctcctggttg
     2581 tatggcctgg tgagtggcgc gatccgtctt tgctataaac taaaactgaa gcgcgcctgg
     2641 cgtgcccccg taccggttgt cgtggttggt aatctcaccg caggcggcaa cggaaaaacc
     2701 ccggtcgttg tctggctggt ggaacagttg caacagcgcg gtattcgcgt gggggtcgta
     2761 tcgcggggat atggtggtaa ggctgaatct tatccgctgt tattgtcggc agataccacc
     2821 acagcacagg cgggtgatga acctgtgttg atttatcaac gcactgatgc gcctgttgcg
     2881 gtttctcccg ttcgttctga tgcggtaaaa gccattctgg cgcaacaccc tgatgtgcag
     2941 atcatcgtaa ccgacgacgg tttacagcat taccgtctgg cgcgtgatgt ggaaattgtc
     3001 gttattgatg gtgtgcgtcg ctttggcaat ggctggtggt tgccggcggg gccaatgcgt
     3061 gagcgagcgg ggcgcttaaa gtcggttgat gcggtaatcg tcaacggcgg tgtccctcgc
     3121 agcggtgaaa tccccatgca tctgctgccg ggtcaggcgg tgaatttacg taccggtacg
     3181 cgttgtgacg ttgctcagct tgaacatgta gtggcgatgg cggggattgg gcatccgccg
     3241 cgcttttttg ccacgctgaa gatgtgtggc gtacaaccgg aaaaatgtgt accgctggcc
     3301 gatcatcagt ctttgaacca tgcggatgtc agtgcgttgg taagcgccgg gcaaacgctg
     3361 gtaatgactg aaaaagatgc ggtgaaatgc cgggcctttg cagaagaaaa ttggtggtat
     3421 ttgcctgtag acgcacagct ttcaggtgat gaaccagcga aactgcttac gcaactaacc
     3481 tcgctggctt ctggcaacta gttacgccgc ggcagcgttc gattgatgga gtcatgaatg
     3541 tcgctgccgc acctctccct tgctgatgcg cgtaatcttc accttgccgc acaaggcctg
     3601 ttaaacaaac cccgccgtcg agcgtcgttg gaggatattc cggcaacgat ctcccgcatg
     3661 tccttgctgc aaatcgatac catcaatatt gttgcccgta gtccatatct ggtgcttttc
     3721 agtcgtctgg gaaattatcc tgcccagtgg ctggatgagt ctctggcgcg tggcgaatta
     3781 atggaatact gggcgcatga agcctgcttt atgccgcgta gcgactttcg tcttattcgc
     3841 caccgcatgc tggcacctga aaaaatgggc tggaaataca aagacgcctg gatgcaggaa
     3901 catgaggcgg aaattgcaca gttaattcag catattcatg ataaagggcc ggtacgttca
     3961 gccgattttg agcatcctcg taaaggtgca agcggctggt gggaatggaa gccgcataaa
     4021 cggcatctgg aaggtttatt tactgccgga aaggtgatgg tgattgaacg gcgcaacttc
     4081 cagcgcgttt atgatttaac ccaccgtgtc atgcctgact gggatgatga gcgcgatctc
     4141 gtttcgcaaa cagaagcaga aatcatcatg ctggataaca gtgcgcgtag cctgggaata
     4201 ttccgcgaac agtggctggc agattactat cggctgaaac gtccggcact ggcggcgtgg
     4261 cgcgaagcga gggctgaaca gcagcaaatc attgctgtgc atgttgaaaa attgggcaat
     4321 ctttggctgc atgctgattt gctgccgcta ctcgagcgtg cgctggccgg aaagctcact
     4381 gcaacgcaca gcgcggtact ttcgcctttt gatcctgttg tctgggatcg caaacgcgca
     4441 gagcagcttt ttgattttag ctaccggctg gagtgctata ccccagcgcg cgaaacgcca
     4501 gtatggctat tttgttctgc cattattaca tcgtgggcaa ttagttgggc gaatggatgc
     4561 caaaatgcat cgccagacag gcatccttga agttatctct ctgtggttac aggaaggcat
     4621 taaaccaacg acaatgctgc aaaaagggtt acgtcaggcg attactgatt tcgcgagctg
     4681 gcagcaggca acgcgggtga cattaggacg ctgcccgcaa ggtctcttta ctgattgccg
     4741 cactggctgg gaaatagacc ccgtcgcata aatgattatg ataagattta tgcattcata
     4801 acccgatcca tctggaggaa ctatggatca tcgtttgctt gaaatcattg cctgcccggt
     4861 ttgcaacgga aaactttggt ataaccagga aaaacaagag cttatttgca aactggataa
     4921 cctggctttc ccgctccgcg atggcattcc agtgttgctg gaaacggaag cccgcgtgct
     4981 gactgctgat gagagtaaat catgagtttt gtggtcatta ttcccgcgcg ctacgcgtcc
     5041 acgcgtctgc ccggtaaacc attggttgat attaacggca aacccatgat tgttcatgtt
     5101 cttgaacgcg cgcgtgaatc aggtgccgag cgcatcatcg tggcaaccga tcatgaggat
     5161 gttgcccgcg ccgttgaagc cgctggcggt gaagtatgta tgacgcgcgc cgatcatcag
     5221 tcaggaacag aacgtctggc ggaagttgtc gaaaaatgcg cattcagcga cgacacggtg
     5281 atcgttaatg tgcagggtga tgaaccgatg atccctgcga caatcattcg tcaggttgct
     5341 gataacctcg ctcagcgtca ggtgggtatg gcgactctgg cggtgccaat ccacaatgcg
     5401 gaagaagcgt ttaacccgaa tgcggtgaaa gtggttctcg acgctgaagg gtatgcactg
     5461 tacttctcac gtgccaccat tccttgggat cgtgatcgtt ttgcagaagg ccttgaaacc
     5521 gttggcgata acttcctgcg tcatcttggt atttatggct accgtgcagg ctttatccgt
     5581 cgttacgtca actggcaggc aagtccgtta gaacacatcg aaatgttaga gcagcttcgt
     5641 gttctgtggt acggcgaaaa aatccatgtt gctgttgctc aggaagttcc tggcacaggt
     5701 gtggataccc cggaagatct tgagcgcgtt cgcgctgaaa tgcgctaatt tcacttcacg
     5761 acacttcagc caattttggg aggagtgtcg taccgttacg attttcctca atttttcttt
     5821 tcaacaattg atctcattca ggtgacatct tttatattgg cgctcattat gaaagcagta
     5881 gcttttatga gggtaatctg aatggaacag ctgcgtgccg aattaagcca tttactgggc
     5941 gaaaaactca gtcgtattga gtgcgtcaat gaaaaagcgg atacggcgtt gtgggctttg
     6001 tatgacagcc agggaaaccc aatgccgtta atggcaagaa gctttagtac gcccggaaaa
     6061 gcccgacaac tggcatggaa aaccaccatg ctggcaagaa gtgggactgt ccgtatgccg
     6121 actatttatg gtgtgatgac gcatgaagaa caccccggcc ctgatgtcct gctactggag
     6181 cggatgcgtg gtgtttcggt ggaggcacca gcccgaacac cagaacgctg ggaacagctc
     6241 aaagaccaaa tcgttgaagc cttactggcc tggcaccgtc aggacagtcg cggttgcgtc
     6301 ggcgcggtcg acaatactca ggaaaatttc tggccctcat ggtaccggca acatgttgaa
     6361 gtgctatgga ccacgctcaa tcagttcaat aacaccggtc tgacgatgca ggataagcgg
     6421 atcctgtttc gcactcgcga atgtctcccg gcattatttg aaggctttaa cgacaattgt
     6481 gtgttgattc acggtaactt ctgtttacgc agcatgctga aagattcgcg cagcgatcag
     6541 ttactggcga tggtcgggcc gggactaatg ctttgggcac cgcgagaata cgaactgttc
     6601 cgactaatgg ataattctct ggcggaagat ttgctctgga gttacctgca acgcgcgcca
     6661 gtggcggagt cgttcatctg gcggcgttgg ttgtatgtgt tatgggatga agttgcgcaa
     6721 ctggttaata ccggacgatt tagtcggcgc aacttcgatc tggcatcaaa atcactcttg
     6781 ccgtggctcg cctgacggac ctttcagcca ctgccagatg cggccaagcg tttcatagcc
     6841 aacacgatcg ctatgcatca gccagaccgg agaagggatt gcccgttccc acggattaag
     6901 cggcgagtcg atggccaact gattcgccgg ggctggtagc ggatttaacc cttcctgctg
     6961 aaaaaagatc atcgcccgcg gcagatgtga tgcggaggtc accagcagga aaggggcgtc
     7021 accaatcgcc tgtttcactg ccgcagcttc ttcttcggta tcttttggca aatccagggt
     7081 gataatttgc tcgcgcggta cgcccagcga ttgcgcaact ctggcaccta cttccgctgt
     7141 actcaccgta ttggtttttg ctacgcctcc cgtgaagatc agttttgaac ccggattttc
     7201 gcgccataaa cggataccct cattcaggcg tggcagactg ttattgatta aattagagct
     7261 cggtgcccac tgcgggttcc aggtataacc acctccgagc accacgatat agtccacttt
     7321 ttgcgaatta ttccatgtcg gataggtgct ttcgatggga cgcagcaggc ggtcggctac
     7381 cggttgcaaa ctcaataaca agagtgccag ccacccaatg ctaataaaaa tcttcccggt
     7441 tttttgaaaa cggctaaacc acagcagcgc caggccagcg ccaataatta acaacatcag
     7501 cggaagtggc agcaacatat tgccaatcac ttttttcagt gtaaaaagca tcctttctgg
     7561 ttcctttttt aaccatatag caagggatcg ccagcgatat tacaccagac gggttcattc
     7621 tcgccgtggc tgtgacaaaa tagcgtcttt atcagaaaac gtcttagcca accggtggag
     7681 aaagcaatgc aggatcgcaa ttttgatgat attgcggaaa agttttcccg taacatttac
     7741 ggcaccacca aagggcagct tcgacaggct attctgtggc aggatctcga tcgcgtgctg
     7801 gcggaaatgg gcccgcaaaa actgcgtgtg ctggatgctg gcggcggaga agggcagacc
     7861 gcaatcaaaa tggccgagcg cgggcatcag gttattttat gcgatctttc tgcgcagatg
     7921 atcgaccgcg caaaacaggc ggcagaagca aaaggtgtga gcgacaacat gcaatttata
     7981 cattgcgccg ctcaggatgt tgcttcgcat ttggaaacgc ccgttgatct gatattgttc
     8041 catgcggtgc tcgagtgggt ggccgatccc cgcagtgtat tacagaccct gtggtcagtg
     8101 ttgcgtctag gcggagtgtt gtcgttaatg ttctacaatg cgcatggttt gttgatgcat
     8161 aacatggtcg ccgggaattt tgattacgtg caggcgggaa tgccgaaaaa gaaaaaacgg
     8221 acgctttcgc cagattatcc acgcgacccg gcgcaggttt atctgtggct ggaagaagct
     8281 ggttggcaaa ttatgggtaa gacaggcgtt cgcgtgtttc atgattatct gcgcgagaag
     8341 caccagcagc gcgactgcta tgaagcatta cttgaattag aaacgcgtta ttgccgtcag
     8401 gaaccgtata ttaccctagg gcgttatatt catgtcaccg cgcgcaaacc gcagagcaag
     8461 gataaagtat gagtgaattt tcccagacag tccccgaact ggttgcctgg gccagaaaaa
     8521 atgacttctc catctcgctg ccggtagacc gactctcttt tctgctggcg gttgccacgc
     8581 tgaacggcga gcgtctggat ggtgagatga gtgaaggcga gctggtggat gcattccgcc
     8641 atgtgagtga tgcgtttgag caaaccagcg aaaccatcgg cgtgcgcgcc aataacgcga
     8701 tcaacgacat ggtgcgtcaa cgtctgctga accgctttac cagcgagcag gcggaaggga
     8761 acgcaattta ccgtctgacg ccgctcggca tcggcattac tgactactac atccgtcagc
     8821 gcgagttttc tacgctgcgt ctttctatga agttgtcgat tgtggcgggt gagctcaaac
     8881 gcgcagcaga tgccgccgaa gagggcggtg atgaatttca ctggcaccgt aatgtctatg
     8941 cgccactgaa atattcggta gcagaaattt tcgacagtat cgacctgacg caacgtctga
     9001 tggacgaaca gcagcagcag gtgaaggacg atatcgccca gttgctgaac aaagactggc
     9061 gggcggcgat ttccagctgt gaattgttgc tttcggaaac ttccggaacg ctgcgtgaat
     9121 tgcaggatac gctggaagcg gcaggcgaca aattgcaggc taatctgttg cgcattcagg
     9181 atgcgacgat gacccatgac gatctgcatt tcgtcgatcg tctggtgttc gatctgcaga
     9241 gcaaactcga tcgtattatc agttggggcc agcaatccat cgacttgtgg attggctacg
     9301 accgccacgt acacaaattt attcgtaccg cgatcgatat ggataaaaac cgcgtctttg
     9361 ctcagcggtt acgtcagtcg gtacaaacct attttgatga gccgtgggcg ctaacttatg
     9421 ccaatgccga tcgtctgctg gatatgcgtg acgaagagat ggcactgcgc gatgaagaag
     9481 tgactgggga acttcctgag gatctggaat acgaagagtt taacgagatc cgcgaacagc
     9541 tggcggcgat catcgaagaa caacttgccg tgtacaaaac cagacaagtg ccgctggatc
     9601 ttggtctggt ggtacgcgaa tatctgtcac agtatccgcg tgcacgtcac tttgacgttg
     9661 cgcgtattgt tattgatcag gcggtacgtc ttggcgtagc gcaagcagat ttcaccggac
     9721 tgccagcgaa atggcagccg attaatgatt acggagccaa ggtacaggcg catgtcatcg
     9781 acaaatattg aacaagtgat gccggttaag ctggcgcagg cgctggcgaa tccgttattt
     9841 ccggcgctgg acagcgcctt acgttcaggc cgccatattg gcctcgacga actggataat
     9901 catgcattcc tgatggattt tcaggaatat ctggaagagt tttacgcgcg ttataacgtt
     9961 gagcttattc gcgcaccgga agggttcttc tatttacgcc cacgttccac cactcttatc
    10021 ccgcgttccg tcttgtcgga actggatatg atggtcggga aaatcctctg ttatctctat
    10081 ctcagcccgg aacggctggc gaatgagggg attttcaccc agcaggaact gtacgacgaa
    10141 ctgctcaccc tggccgatga agcgaaactg ctgaaactgg tgaacaaccg ttcaaccggt
    10201 tcagacgttg accgtcagaa gttgcaggag aaagtacgtt cttcgctcaa ccgtctgcgt
    10261 cgtttaggca tggtgtggtt tatgggccac gacagcagca agttccgcat taccgaatcg
    10321 gtgttccgct tcggagccga tgtgcgtgct ggcgacgatc cccgtgaagc acagcgtcgc
    10381 ctgattcgtg atggcgaagc aatgccgatt gaaaatcatc tgcaactcaa cgatgaaacc
    10441 gaagagagtc agccagatag cggagaggaa gaataatgat tgaacgcggt aaatttcgct
    10501 cactgacgct gattaactgg aacggctttt ttgcccgaac ttttgacctt gacgagctgg
    10561 tcacgacgct ttccggcggt aacggggcgg gtaaatccac cacgatggcg gcgttcgtta
    10621 cggcgctgat ccccgacctg accctgctgc atttccgtaa cactacggaa gccggggcca
    10681 ccagcggttc gcgcgataaa ggtctgcacg gtaagctgaa agcgggtgtc tgttattcga
    10741 tgctcgacac cattaactcg cgccaccagc gcgtggtggt cggtgtgcgt ctgcaacagg
    10801 ttgccggacg cgatcgtaaa gtggatatca aaccatttgc cattcaggga ctgccgatgt
    10861 cggtgcagcc gacacagctg gtgaccgaaa ccctgaacga acgccaggcg cgcgtgctgc
    10921 cgcttaacga gctgaaagac aagctcgagg cgatggaagg cgtgcagttt aaacagttca
    10981 actccattac cgattaccac tcgctgatgt tcgatctggg catcatcgcg cgtcgtctgc
    11041 gctccgcatc tgaccgtagc aaattctatc gtctgatcga agcttcgctg tatggcggga
    11101 tctccagtgc cattacccgt tctctgcgcg actacctgtt gccggaaaac agcggcgtgc
    11161 gtaaagcgtt ccaggacatg gaagcggcgc tgcgtgaaaa ccgcatgacg ctggaagcga
    11221 ttcgtgtcac ccagtcggac cgtgacctgt ttaagcatct gatcagcgaa gctaccaact
    11281 acgtggcggc ggactacatg cgtcacgcca acgagcgccg tgtccatctc gacaaagccc
    11341 tggagtttcg tcgcgagcta catacttcgc gtcagcaact ggcggctgag cagtacaaac
    11401 acgtcgatat ggcgcgtgag ctggcagagc acaacggtgc cgaaggcgat ctggaagcgg
    11461 attatcaggc ggccagcgat cacctgaacc tggtgcaaac cgcattgcgt cagcaggaga
    11521 agatcgaacg ctacgaagtg gatctcgatg agctgcagat ccgtctggaa gagcaaaatg
    11581 aagtggtggc agaagccatc gaacgccagg aagagaatga ggctcgcgcg gaagctgccg
    11641 aactggaagt ggacgagctg aaaagccagc ttgctgacta ccagcaggcg ctggacgtcc
    11701 agcaaacgcg cgcgatccag tataaccagg cgattgctgc gcttaatcgt gccaaagaac
    11761 tgtgccatct gccggactta accgccgaca gcgccgccga atggctggaa accttccagg
    11821 cgaaagagct ggaagcgact gaaaaaatgc tctctcttga gcagaaaatg agcatggcgc
    11881 aaaccgcgca cagccagttt gagcaggctt atcagctggt ggtggcaatc aacggcccgc
    11941 tggcgcgtaa cgaggcgtgg gatgtcgctc gcgaactatt gcgcgaaggg gttgatcagc
    12001 gccacctggc agagcaggtt cagccgttgc ggatgcgctt aagcgaactg gaacagcgtc
    12061 tgcgtgagca gcaagaagct gagcgtctgc tggcagattt ctgcaaacgt cagggcaaga
    12121 attttgatat tgatgaactg gaagctctgc atcaggagct ggaagcacgc attgcctctc
    12181 tttccgatag cgtatctaac gcccgtgaag agcggatggc actgcggcag gaacaggaac
    12241 agctgcagtc tcgcattcag agtttgatgc agcgtgcacc ggtttggctg gcagcgcaaa
    12301 acagtctcaa ccagttgagt gaacagtgcg gcgaagagtt ctcctccagc caggacgtca
    12361 ctgaatatct gcaacagttg ctggagcgtg agcgcgaggc gattgttgaa cgcgatgaag
    12421 taggcgcgcg caaaaacgcc gtcgatgaag agatcgaacg tttaagccag cctggcggct
    12481 ctgaagatca gcgtctgaac gcgctggcgg agcgttttgg tggtgtgctg ctgtcagaaa
    12541 tttatgacga cgttagcctg gaagatgcgc cgtacttctc ggctctgtat ggcccgtcac
    12601 gccacgccat cgtggtgcca gatctgtcac aggtaaccga acacctggaa ggcttgaccg
    12661 attgcccgga agatctctat ttgatcgaag gggatccgca gtcattcgat gatagcgtgt
    12721 tcagcgttga tgagctggaa aaagcggtgg tggtgaaaat cgccgatcga cagtggcgtt
    12781 attcacgttt cccggaagtg ccgctgtttg gtcgtgctgc gcgtgaaagc cgtattgaaa
    12841 gcctccatgc cgagcgtgaa gtgctttccg aacgcttcgc cacgctctcc tttgatgtac
    12901 agaaaaccca gcgtctgcat caggcgttca gccgctttat cggcagtcat ctggcggtgg
    12961 cgtttgagtc tgacccggaa gcagaaatcc gtcaactgaa cagccgtcga gtcgaactgg
    13021 agcgggcgtt gagtaatcat gaaaatgata accagcagca gcgtattcag tttgagcagg
    13081 cgaaagaagg cgttacggcg ctgaaccgca ttctgccgcg tctcaacctg ttggctgatg
    13141 acagcctggc ggatcgcgtc gatgaaatcc gcgaacgtct ggatgaagcc caggaagccg
    13201 cgcgttttgt tcagcagttt ggcaatcaac tggcgaaact ggaaccgatc gtttcggtat
    13261 tgcagagcga cccggaacag ttcgaacagt taaaagaaga ttacgcgtac tctcagcaga
    13321 tgcagcgcga tgcccgtcag caagcgtttg ccctgacgga agtggtgcag cgtcgtgcgc
    13381 actttagcta ttctgactcg gcagaaatgc ttagcggtaa cagcgatctc aacgaaaaac
    13441 tgcgtgaacg tctggaacag gcggaagcgg agcgtacccg cgctcgcgaa gcgttgcgcg
    13501 gtcacgcagc gcagttgagt cagtacaacc aggtgctggc ttcgctgaaa agttcttacg
    13561 acaccaaaaa agagctactc aacgatctgc aacgtgaatt gcaggatatc ggcgtgcgtg
    13621 ctgatagagg ggcagaagag cgggcgcgta ttcgccgtga cgagctgcat gcgcaactga
    13681 gcaataaccg ttcacgccgc aatcaactgg aaaaagcgct taccttctgc gaagcggaga
    13741 tggacaacct gacccgcaaa ctgcgcaagc tggagcggga ttactttgag atgcgcgagc
    13801 aggtagtgac cgccaaagcg ggctggtgtg cggtgatgcg catggtgaaa gataacggcg
    13861 ttgagcgccg cttacaccgt cgtgagctgg cttatctctc cgctgatgat ttgcgttcca
    13921 tgtcggataa ggcgttaggt gcgctgcgtc tggcggtggc ggataacgaa catctgcgcg
    13981 acgtgctgcg catgtcggaa gatccgaaac gtccggagcg taaaattcag ttcttcgtgg
    14041 cggtttatca gcatctgcgt gaacgtattc gtcaggatat tattcgtacc gatgatccgg
    14101 tggaagctat cgaacagatg gagattgaac ttagccgtct gaccgaagaa ttaacctccc
    14161 gtgaacagaa actggcgatc agttcccgca gcgtggcgaa catcattcgc aaaaccattc
    14221 agcgcgagca gaaccgtatc cgtatgctca accaggggtt gcagaacgta tcgtttggtc
    14281 aggtgaacag cgtgcgtctc aacgtgaacg tgcgtgaaac gcacgccatg ctgctggatg
    14341 tgctctctga acagcacgag cagcatcagg atctgtttaa cagcaaccgt ctgaccttct
    14401 cggaagcgtt ggcgaaattg tatcagcgtc ttaatccgca gattgatatg gggcagcgca
    14461 cgccgcagac cattggcgaa gaactgctgg attaccgcaa ctatctggaa atggaagttg
    14521 aggttaaccg tggttccgat ggttggttgc gcgcagagtc tggtgcattg tcgaccggtg
    14581 aggcgattgg taccggtatg tcgattctgg tgatggtggt acaaagctgg gaagatgaat
    14641 ctcgccgcct gcgcggtaaa gatatctctc cttgccgcct gctgttcctc gatgaagcag
    14701 cgcgactgga tgctcgttct atcgccacgc tgtttgaatt gtgtgaacgt ttgcaaatgc
    14761 agctcatcat cgcagcgccg gaaaatatca gtccggaaaa aggcaccacc tataaactgg
    14821 tacgtaaagt cttccagaat accgaacatg ttcacgtcgt cggcctgcga ggatttgcgc
    14881 cgcaactccc tgaaacgctt ccaggaagtg acgaagcgcc ttcccaggcg agttaaaatt
    14941 aaggcggcag caatgccgcc ttttcttttt ccgaaaactc cgtttctgca ctaaaaaaat
    15001 ggcacataac ggcgtaatgc cgatcagtta a
//
LOCUS       DBKEKI010000090        14896 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.090, whole genome shotgun sequence.
ACCESSION   DBKEKI010000090 DBKEKI010000000
VERSION     DBKEKI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.090"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1054
                     /gene="glxK"
                     /locus_tag="ACUQPX_002813"
     CDS             <1..1054
                     /gene="glxK"
                     /locus_tag="ACUQPX_002813"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HHV9057474.1"
                     /translation="PDAHYICLPIADGGEGTVDAMVAATGGNIVTLEVCGPMGEKVNA
                     FYGLTGDGKTAVIEMAAASGLMLVAPEKRNPLLASSFGTGELIRHALDNGIRHIILGI
                     GGSATVDGGMGMAQALGVRFLDADGQVLAANGGNLARVASIEMEECDPRLANSHIEVA
                     CDVDNPLVGARGAAAVFGPQKGATPEMVEELEQGLQNYARVLQQQTEINVCQMAGGGA
                     AGGMGIAAAVFLNADIKPGIEIVLNAVNLAQAVQGAALVITGEGRIDSQTAGGKAPLG
                     VASVAKQFNVPVIGIAGVLGDGVEVVHQYGIDAVFSILPRLAPLAEVLASGETNLFNS
                     ARNIACAIKIGQGIKN"
     gene            complement(1282..2067)
                     /gene="allE"
                     /locus_tag="ACUQPX_002814"
     CDS             complement(1282..2067)
                     /gene="allE"
                     /locus_tag="ACUQPX_002814"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HHV9057475.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGTSFVDYLVTLHQNGGNQQCFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPSSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(2078..3313)
                     /gene="allC"
                     /locus_tag="ACUQPX_002815"
     CDS             complement(2078..3313)
                     /gene="allC"
                     /locus_tag="ACUQPX_002815"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HHV9057476.1"
                     /translation="MITHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FADAMKACEFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(3335..4384)
                     /gene="allD"
                     /locus_tag="ACUQPX_002816"
     CDS             complement(3335..4384)
                     /gene="allD"
                     /locus_tag="ACUQPX_002816"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HHV9057477.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKVAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            4701..6368
                     /locus_tag="ACUQPX_002817"
                     /pseudo
     CDS             4701..6368
                     /locus_tag="ACUQPX_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
     gene            6378..7637
                     /locus_tag="ACUQPX_002818"
     CDS             6378..7637
                     /locus_tag="ACUQPX_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HHV9057478.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            7648..8463
                     /locus_tag="ACUQPX_002819"
     CDS             7648..8463
                     /locus_tag="ACUQPX_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HHV9057479.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            8460..9353
                     /locus_tag="ACUQPX_002820"
     CDS             8460..9353
                     /locus_tag="ACUQPX_002820"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HHV9057480.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9490..10557)
                     /gene="purK"
                     /locus_tag="ACUQPX_002821"
     CDS             complement(9490..10557)
                     /gene="purK"
                     /locus_tag="ACUQPX_002821"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HHV9057481.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWLAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(10554..11063)
                     /gene="purE"
                     /locus_tag="ACUQPX_002822"
     CDS             complement(10554..11063)
                     /gene="purE"
                     /locus_tag="ACUQPX_002822"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HHV9057482.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(11181..11903)
                     /gene="lpxH"
                     /locus_tag="ACUQPX_002823"
     CDS             complement(11181..11903)
                     /gene="lpxH"
                     /locus_tag="ACUQPX_002823"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HHV9057483.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(11906..12400)
                     /gene="ppiB"
                     /locus_tag="ACUQPX_002824"
     CDS             complement(11906..12400)
                     /gene="ppiB"
                     /locus_tag="ACUQPX_002824"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HHV9057484.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            12574..13959
                     /gene="cysS"
                     /locus_tag="ACUQPX_002825"
     CDS             12574..13959
                     /gene="cysS"
                     /locus_tag="ACUQPX_002825"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HHV9057485.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(13995..14516)
                     /locus_tag="ACUQPX_002826"
     CDS             complement(13995..14516)
                     /locus_tag="ACUQPX_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HHV9057486.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWRVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(14624..14836)
                     /gene="ybcJ"
                     /locus_tag="ACUQPX_002827"
     CDS             complement(14624..14836)
                     /gene="ybcJ"
                     /locus_tag="ACUQPX_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HHV9057487.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
BASE COUNT         3461 a         3856 c         4154 g         3425 t
ORIGIN      
        1 tcccgatgcg cactatatct gtttgccgat agcggatggc ggcgaaggga cggtggatgc
       61 gatggtcgcc gcgacgggtg gcaacatcgt gacgcttgaa gtctgcgggc cgatgggcga
      121 aaaagtgaat gctttttatg gccttaccgg agacgggaaa acagcggtga ttgagatggc
      181 ggcagcaagt ggcctgatgc tggtcgcgcc tgaaaagcgt aatccgttgc tggcctccag
      241 ttttggtacg ggggagttaa ttcgccatgc gctggataac ggcattcgcc atattattct
      301 cggcattggc ggcagtgcga cggtcgacgg cggtatgggc atggcgcagg cgcttggtgt
      361 gcgtttcctt gatgccgacg gccaggtgct ggcggctaac ggcggcaatt tagcgcgcgt
      421 ggcaagcatt gagatggagg aatgcgatcc gcgtctggcg aacagccata ttgaagtggc
      481 atgtgacgtt gataacccgc tggtaggggc acgcggcgcg gcggcggtat ttggcccgca
      541 aaaaggggca acgccggaga tggtcgaaga gctggaacag gggctgcaaa attacgcccg
      601 tgttttacaa cagcaaactg aaattaatgt ctgtcagatg gcgggcggcg gcgctgcggg
      661 cggtatgggt attgcggcgg cggtatttct caatgcggat attaaaccgg gcattgagat
      721 tgtgttgaat gcggtcaatc ttgcgcaggc ggtgcagggg gcagcactgg tgatcaccgg
      781 ggaagggcgc attgactcgc aaacggcagg cggtaaagcg ccgctgggcg ttgcgtcggt
      841 ggcgaagcag tttaatgtac cggtgattgg gattgctggt gtattgggtg atggcgtgga
      901 agtggtgcac cagtacggca ttgacgcggt attcagcatt ttgcctcgtc tggcaccttt
      961 agccgaagtg ctcgccagcg gtgaaaccaa tctcttcaac agcgcgcgaa atattgcctg
     1021 cgccattaaa ataggtcagg gaattaaaaa ctgactcttg cctttaaagc ggatgcgatt
     1081 tatttcgtat aagagcgcag tactcatgcc ggatgcggca tgagtactat atccttcctg
     1141 aaaatcgcgc aaattctata tattgcagag gtcatgtagg cctgataagc gaagcgcatc
     1201 aggcaatgtt acaaaaaaag ccacggtata aaccgtggca aaatccaaca tagctaaaaa
     1261 taatcaggcg agtggtatga cttaaatctc tacgtcgcgg ttgcaatctt tcgagtaaat
     1321 atagctgaac gcttcaccac gccctacgcc ataaccagcc tgtaaagaat aagcgcccat
     1381 aaagatgtaa tcgccttttt tcaccgggat ccagttattg tcgaggttat aaaccccctg
     1441 accggaaaga atataggcac catgttcctg aacgtgtgtt tcgatataac cgtggctggc
     1501 acctggtgca aaagagagga tatgcatgtt catatcaaaa cctaactctt tgggcagaaa
     1561 atccagcagg atcacatcat ccataccttc atagtgaatg cgttccagtt cgctggcatt
     1621 gccagaaacc agccacggtg catggccttc taccggaaca tagcggcgct tatataaaaa
     1681 gatttggctg tcttcggcct gggcgttaac aaacgtcatt aaggagcttg gtgggcaata
     1741 aagatagcca ccttcgctta aggcaaatgt tttgccttcg gctttggcag tgatatttcc
     1801 agagatcaca tacaggaacg tttcaatgcc ttcgccaccg aagcactgtt ggttgccacc
     1861 gttttgatgc agtgtgacca gataatcaac aaaagaggta cccagctttg gcgtggagag
     1921 gattgtcgcg tcacaatttt caaagcccgg aataatattt tttaccagac catccggggt
     1981 taacagtgcg aaattaccgt gtttaacaat cgcacggtta gccagtaaat cttcgcggta
     2041 accggtgacg ttatttaaat atcccattta tgactcctta tttctgccag gcaagttgat
     2101 aaagcatgag tgccaacgtt ttgacccctt cggcaaggtc agtaatattg gtgcgttccg
     2161 ccgggttatg gctgatcccg ttgatgctgg ggataaaaat catgcaggtt ggtacgcgag
     2221 gcgcgaaaat ttgcgcgtcg tgcccggcac cactgtgcat cacccggtaa ttcagttttt
     2281 ctctttcaca caattctgtc agggtggcga ccagctcctt attcatcggc acgggttctt
     2341 cgtccatcca taaatcgata tcaataccaa tgtccatttc atcgcaaatc gcccgcatgt
     2401 cgttttctaa ctgttgggtg aaatcgcgca gcacggcagc gtcggtatga cgacaatcaa
     2461 tggtgaacgt ggttttaccc ggcaccacat ttaccgtatt cgggcgcggc tctactttgc
     2521 caaaggtcag aaccagcgga tcgcccatct ttttcgcttt ttcgatcgac tgatggcaaa
     2581 tgcgactgaa agcgtaaacc gtatcacgac gataacccat cggcgtggtg cctgcatggt
     2641 ttgattcgcc gttcagcgtc accgtatagc gacgctgccc gacaattgca ttcaccacgc
     2701 caattgattg cccattactt tccagcacac agccctgttc aatatgcagt tcaacaaagg
     2761 cttttatatc ctgacgcgga gttagtgggg cgttcggcag agtaaattcg caagccttca
     2821 tcgcatcggc aaaactattt cctttggcat cacagatatt ccgcacgtcg tcaggattcg
     2881 ccagcccaaa gatattttta ctgccccaga agacatacgg gaagcggctg ccttcttctt
     2941 ctgccatcgc caccacttcg accgtacgta acggtgcgcc gtattgcgtt ttcaaccagt
     3001 caattgccag ccacgccgcc agcgcgccga attgcccgtc gaggttaccg ccgttaacca
     3061 cggtatcgat atgcgaaccg ctcagaacca cttcctgtgg atattcggtg ccactcaggc
     3121 gaccgtataa attccccact tcatcgaaac gtgtttccag cccacttgct gccattcttt
     3181 ttttaaattg ctgctgggtt tccagccatt ccggcgaata aagtaaacgg gtcatcccac
     3241 ccgttgggtc agcgccaaaa gaggaaagcc agggcagcgt ttcttctata gcttgacggg
     3301 aatgtgtaat cataagaaag tcctgtctca ataattattg cgcaaaggga tttttcgttt
     3361 cgtatgacgt gttataaagc gcgtcggaaa ttaaatactg gtaaatatca tcaacaattt
     3421 cgatgccttc gacggcgact ttgcgttgtt taatatcctg atcctgtccg ggataataaa
     3481 cctgattaaa accgggcgcg ggggtaatgg catttaattc gcgcatggtc tggctaagat
     3541 gttgacggaa taattcgctg gaggagaaaa agttcggatt aataactata tgtaattgcc
     3601 ccaaattacg ccctgcgtgt aaatcgtcat acatcgaact aacctgtcgc ccgaacggta
     3661 agccgagtaa gacgcctgag aggacgtcaa tcatcatcat caggccatac ccttttggcc
     3721 cagcggcggg gagcagagca tgtaccgcga acggatcggt tgttggtgca ccgtttttat
     3781 cgaccgccca ggtatccggg atagacatat tacgcgagcg ggcgtcgagc acttttcccc
     3841 atgcctgtac ggtagtcgcc atatcaaagg taaggatctc gtcgccttct cccggtgcgg
     3901 caaaggccag tgggttagta ccgtagtaaa tttccgcgcc gccaaacggc accaccattg
     3961 gatcggactg gcacatcgaa atgccaatta atccggcgcg ggctgcctgc tgcacaaaat
     4021 aagagattgc gccgctgtga cccatccggc tgataccgac caccgcaacg cctttttgct
     4081 gggcggtttt gatggcatgt tccataccca ttttcgccgc gacctgcccg gcggcattgt
     4141 cagcatgtaa aattgccgag cacggcccgg tttcctcaag acgaaactcc ggttcgcggt
     4201 tggtgccgcc ttttgaaatg cgttccgcgt agtattccac gcgcaccgcg ccatgagagt
     4261 ggatccctct ggcatcggcg taaaccaata cttcagccac ggttgcagcg tgctcacgtt
     4321 ttaacccagc ctggcagagt ttattctcga ttagctggtg gagtgtttcc cgactaattt
     4381 tcatctgtct tcctttttaa cgacggtgtg aagcatgact gcaattaaca tacagggaaa
     4441 atatctggat tatgtgatcc agacaggcaa aaaaatatag ttagaattta tttgataatc
     4501 cgctcgcttt taacctggtt tttaaaacaa ccacgcttat taaaaaataa cacgtaatag
     4561 aatggcggtt atttgaattc ttttgttaat aatccttgtg tgatattcat caccttattt
     4621 acccgttgtc atcgataccg taatcgccac attaacactg ctcgtgcaat tgccatgggt
     4681 gcaattttta aggagttgtt atgatccacg cctttattaa aaaagggtgt tttcaggatt
     4741 cggtcagttt aatgattatt tcacgaaaac tcagcgaatc agaaaatgtt gatgatgttt
     4801 ccgtaatgat gggtacgccc gccaataaag cgttattaga taccacaggt ttctggcatg
     4861 acgattttaa taacgccacg ccgaacgata tttgcgtggc aattcgtagc gaagcggcgg
     4921 atgcggggat cgcgcaggcg attatgcagc agcttgaaga ggcgctaaaa caactggcgc
     4981 agggttcagg cagcagccag gcgttgacgc aggtgcgtcg ctgggacagc gcctgtcaga
     5041 aattacccga tgccagtctg gcgctgattt cagtggctgg cgagtatgcg gcggagctgg
     5101 cgaaccaggc gctggatcgc aacctcaacg tgatgatgtt ctccgataac gtcacgctgg
     5161 aagatgaaat ccaactcaaa acccgcgcgc gggaaaaagg cttgctggtg atggggccgg
     5221 actgcggtac atcgatgatt gccgccacgc cgctggcttt tgctaacgtg atgccggaag
     5281 gcaacattgg cgtcattggc gcttccggta ccgggattca ggagctgtgc tcacagattg
     5341 cgctggcagg ggagggaatt actcacgcga ttggccttgg cgggcgcgac ctcagccgtg
     5401 aagtgggcgg catcagtgcg ctgacggcgc tggaaatgct cagtgcagac gagaaaagcg
     5461 aagtgctggc atttgtttca aaaccacctg ccgaagctgt gcgtctgaaa attgttaatg
     5521 ccatgaaagc aaccggcaaa ccgacggtgg cgctgttttt aggttatacc ccggcggtgg
     5581 cacgcgacga gaatgtctgg tttgcctcct cgctggatga ggccgcacgc ctggcttgcc
     5641 tgctttcacg cgttacggcg tgacgtaacg cgataacgcc tgccagcagc ggatttattt
     5701 gcggtttgta taccggcggt acgctggctg ccgaagcggc gggattactt gccggatacc
     5761 ttggcgtgga agtcgatgat gcccatcatc acggcatgat gctcgacgcc gatggtcacc
     5821 agattattga cctgggcgat gatttctaca cagtcgggcg tccccatccg atgatcgacc
     5881 caaccttacg caaccagtta attgccgatc tcggcgctaa accacaagtg cgcgtattgc
     5941 tacttgatgt cgtgattggc ttcggtgcga ctgccgatcc tgccgcctcg ctggtaagcg
     6001 cctggcaaaa agcctgtgcc gcgcgttcag ataatcaacc actgtatgcc attgccacgg
     6061 tgacaggcac tgaacgtgac ccgcaatgcc gctcgcagca aatcgccacg ctggaagatg
     6121 cggggatcgc ggtagtgagt tcgctgccgg aagccacctt gctggcggca gcgttaattt
     6181 atccgctctc gcctgccgca cagcaacata caccgtcatt actggaaaac gtcgccgtga
     6241 ttaacatcgg attacgcagc tttgcgctgg agctacaaag cgccagcaaa ccggttgtgc
     6301 attaccaatg gtcgccagtc gccggcggca ataaaaaact ggctcgttta ttagaacgtt
     6361 tgcaataagg ggttcccatg tttacatcag tggcgcaagc caatgctgcg gttatcgaac
     6421 aaattcgtcg tgctcgtcca cactggctgg atgtgcaacc ggctttttca cttatcagcg
     6481 aactaaacga gggcaaaaca ctgcttcacg ccgggccgcc aatgcgctgg caggagatga
     6541 ccggacccat gaaaggggcg tgcgtgggcg catgtctgtt cgaaggctgg gcgaaagatg
     6601 aagcgcaggc actggcgatg ctggagcagg gggaagtgaa cttcattcct tgtcaccatg
     6661 tgaatgccgt cgggccaatg ggcggcatta cttctgccag tatgccgatg ctggtggtcg
     6721 agaacgtgac cgacggcaac cgggcgtact gcaacctcaa cgaaggtatc ggcaaagtga
     6781 tgcgttttgg cgcttacggc gaagatgtcc tgactcgcca tcgctggatg cgcgatgtgt
     6841 taatgccagt attaagcgcg gcgctggggc gcatggagcg cggtatcgat ctcacggcga
     6901 tgatggcgca gggcattacg atgggcgatg agttccatca acgcaatatt gcttcctcag
     6961 cactgttaat gcgtacgctg gccccacaaa ttgctcgcct cgatcatgat aaacagcaca
     7021 ttgcggaagt gatggatttc ctcagcgtga ccgatcagtt cttcctcaac ctcgcgatgg
     7081 cctactgcaa ggcggcgatg gatgctggcg cgatgatccg cgcaggcagc atcgttacgg
     7141 ctatgacccg caacggcaat atgttcggga ttcgggtaag cgggctgggt gaacgctggt
     7201 ttactgcgcc tgtaaacact ccgcaaggtc tgtttttcac cggcttctcg caggagcagg
     7261 cgaacccgga tatgggcgat agcgcgatta ccgaaacctt tggtatcgga ggtgcggcaa
     7321 tgatcgcagc gcctggcgta acgcgctttg tcggtgcggg tggcatggaa gcggccaggg
     7381 cggtatctga agagatggcg gaaatttacc ttgaacgcaa tatgcagttg cagatccccg
     7441 gctgggattt tcagggcgcg tgcctggggc tggatattcg tcgcgtggta gaaaccggca
     7501 ttacgccact catcaatacc ggtatcgccc ataaagaggc ggggatcggg cagattggcg
     7561 caggcaccgt gcgggcaccg ctggcgtgct ttgaacaggc gctggaagca ctggctgaaa
     7621 gcatggggat tggttgagga acgcgcaatg acgatcatcc atcctctgct tgccagcagt
     7681 agcgcaccga attatcgcca gtcctggcgg ttagcgggag tgtggcggcg ggcgattaac
     7741 ctgatgacgg aaagcggcga actgttaacg ctgcatcgtc agggtagtgg tttcggcccc
     7801 ggaggatggg tgcttcgccg cgcgcaattc gatgcgttat gcggtggatt atgcggcaat
     7861 gaacgaccac aggttgtggc tcaagggatt cgcctcgggc gtttcacggt taaacagcca
     7921 cagcgttatt gtttgctgcg tattacgccg ccttcgcatc ctcaaccgct tgcagctgca
     7981 tggatgcaac gcgcggagga aaccggactt ttcgggccac tggcgatggc ggcaagcgat
     8041 ccgctgcccg ctgaattacg ccagtttcgt cactgttttc aggccgcgct caatggcgtt
     8101 aagaccgact ggcggcactg gctgggtaaa ggccccggat taacgccaag tcatgatgac
     8161 acgttgagcg gaatgctgct ggcggcctgg tattatggcg ctttagatgc gcgcgccggt
     8221 cgcccgtttt ttgcctgttc cgacaatctt caactcgtta ccacagcggt gagcgtcagt
     8281 tatttacgtt atgccgcgca aggatatttc gcctcgccac tcctgcactt tgtccatgct
     8341 ctgagttgcc cgaaacgtac cgctgttgcg attgattcgc tgctggcgct ggggcatacg
     8401 tcaggggcag atacgctgct ggggttctgg cttggccaac aattattaca aggaaaacca
     8461 tgaaaacact ggttgtagct cttgggggca acgccttact ccagcgcggt gaggcgctga
     8521 cggcggaaaa tcaatatcgc aatatcgcca gtgctgtacc cgcgctggca cgcctggccc
     8581 gttcttatcg gctggcgatt gttcacggca acgggccgca ggtggggctg ctggcgttac
     8641 agaatctggc gtggaaagag gtagaaccgt atccgctgga tgtgctggtg gcggaaagcc
     8701 aggggatgat tggctatatg ctggcgcaga gcttgagcgc acagccgcag atgccgcctg
     8761 ttacgacggt gctgacgcgc attgaggttt cgcctgatga tccggcgttt ttgcagccag
     8821 agaaatttat tggtccggtt tatcagccag aagaacaaga ggcactggaa gcggcttacg
     8881 gctggcagat gaaacgtgat ggtaaatatt tgcgccgggt ggtggcgtct ccgcaaccgc
     8941 gtaaaattct cgacagcgaa gccatcgagt tgttgctcaa agaggggcat gtggtgattt
     9001 gcagtggcgg cggcggtgtg cctgtgacgg aagatggggc agggagtgaa gcggtgattg
     9061 ataaagatct cgccgctgcg ttgctcgccg agcagattaa tgccgatgga ctggtgatcc
     9121 tcaccgatgc tgatgcggta tatgaaaact ggggaacgcc gcagcaacgt gccattcgcc
     9181 atgccacacc ggatgagtta gcgccatttg ccaaagccga tggttcgatg gggccgaagg
     9241 taacggcggt gagtggttat gtcagaagcc gtagtaaacc cgcgtggatt ggggcgttat
     9301 cgcgaattga agagacgctg gcgggcgaag cggggacctg tatttcgctg tagtgatgag
     9361 agcatccggc aatcggtgca ccatgcctga tgcgacgctt gacgcgtctt atcaggccta
     9421 caatgggtac cggagcggta agccggataa ggcgtttacg ccgcatccgg caatcaaatg
     9481 agcacctgct taactgaact tactctgcgc ccaaatcacg ccgctggcat actctggcgg
     9541 cagcagcggg attaaggctt ccagcgtcgc agtcagacgc gatgtgtcgc tgtcggtcaa
     9601 attcagatgc cccactttac gccccggacg gacttctttg tcgtaccagt gcagatgcac
     9661 cagcggcaat ttcagccagt cataattcac atcgctacca atcagattga tcatcaccga
     9721 cggattattc actactggtt gcggtaacgg cagatcggta atcgcccgca gatgcagctc
     9781 aaactggctg atgctggcac cgttttgtgt ccagtgaccg ctgttatgca cacgcggagc
     9841 cagttcgttg atcagcagac cttgcggggt gacaaaacac tccatcgcca tcacgcccac
     9901 atagcccagc tcctgcataa tcgccgacag catctcttcg gcttgcgcct gctgctgtgc
     9961 gttggcctgc ggaaaagcga cgctggtgcg caaaataccg tcctgatgca ggttatgcgt
    10021 cagcggataa aacacggtgc tgccatcaaa gccgcgcgcg ccaaccagcg atacttcacc
    10081 agagaagtta atgccctgct cgacaataca ttcaccgtaa cactctgccg gtaactgttc
    10141 ggtttcattg gcgcgtaaac tccattgacc gcgaccgtca tagccaccag tgcgacgctt
    10201 aacaatcgcc agttcaccta aacgatcaaa caccgcaagc cactcgctgc gatcggcaag
    10261 taactgccac ggcgcggtcg gcaggtggag cttatcgaaa agctgtttct gagtcagacg
    10321 gtcagcaata atcgggaaca catcgcggtt cacaaaggcc ggatgacgcg ccagctcgcg
    10381 ggttaatgcg gtttccggcc agcgttcgat ctcagcggta atcacgcttt gttgaaaagg
    10441 caccgccgcc ggttcagcgt ccagcccgac cggccagaca gcaatgccta acggttcgcc
    10501 tgcctgacgc agcatacggc ctaactgccc gttaccgagg acgcaaacct gtttcatgcc
    10561 gcacctcgcg ggtccgggtt ttccagcact tcgtcggtct gggctttgcg ccagtcattc
    10621 agacgctggt gtagctcttt atcgtgagtc gcgagaattt gtgctgccag taatgccgcg
    10681 tttgccgcgc cagctttacc aatcgccagc gtacccaccg gaatgccgcg cggcatttgt
    10741 acgatggagt agaggctatc gacaccgctc agtgcggcgc tctgtactgg cacgcccagc
    10801 accggcacca gcgttttggc ggcaatcatg cctggcaggt gcgctgcgcc gcctgcgccc
    10861 gcaataatca cctgataacc gttctcttcg gcgctttcgg cgaagctgaa cagtttatcg
    10921 ggggtgcggt gagcagaaac cacttcaacg tggtgcggga cattcaggat ttcgaagatt
    10981 tcgacggcga actgcatggt agcccagtcg cttttggaac ccatcacgat ggcgacacgc
    11041 gccggattat tgcgggaaga catgcgtctt aaaactcctg tggtgcacaa ctctcggctt
    11101 tagagggcac agagaatagc acggaaagag agcaaggaaa acggttgcgt ggctgtgaaa
    11161 tcagcaaagt tgtgggtttt ttaaaacgga aaatgaatca gctcaacgtc atccgccgtg
    11221 actttcacca tcgaaccttc cgtatgccag gcacccagta ccacgcgaaa agcaggttgc
    11281 tggttggcga taagttcatg caccgccggg cgatgggtat gcccgtggat cagccattgc
    11341 acctgatgtt tttccatcgc actgaccaca gcgttttggt taacgtccat gatcgccagc
    11401 gatttactgc tgttggcttc tttgctgttc gcgcgcattc gcgcggcaat gcgtttgcgc
    11461 acaaacaacg gcagggcgag gaatagcatt tgcagccagg gtttgtggac tttggcgcga
    11521 aaagcctgat aacccgcgtc atcggtgcac agcgtgtcgc catgcataat caacacccgg
    11581 cgaccataaa gttcgagcac cttttcttcc ggcaataatg tcatgccgct ttcacgggca
    11641 aagcgtttgc cgagcagaaa atcacggttg ccatgaatga aataacaggg aacgccagaa
    11701 tcggacaccg ctttgatcgc tgccgccatc tggcgatgga gtgggttggg atcgtcgtcg
    11761 ccaatccatg cttcaaacag atcgccaaga atatacagcg cgtcggcctt gcgggcttcc
    11821 cccgctaaaa aacgcagaaa accggcggtg atcgccggtt cttccacgca gagatgaaga
    11881 tctgcaataa agagtgtcgc cacgattact cgctaacggt cacgctttca atgataacgt
    11941 cttcttttgg cacgtcctgg tgcataccgt tacgaccggt tgcaacacct ttgattttgt
    12001 ctaccacgtc catgccgtca accacttcag caaacacgca gtagccccaa ccttgcaggc
    12061 tttcgccaga gaagttcagg aagtcgttat caaccacgtt gatgaagaac tgtgcagttg
    12121 cagagtgcgg agcctgagta cgtgccattg ccagcgtacc acgggtattt ttcaggccgt
    12181 tgttggcttc gtttttgatc ggttctttgg tggctttttg tttcatgccc ggttcaaaac
    12241 cgccgccctg aatcataaag ccgttgataa cacggtggaa aatggtgttg ttgtaaaaac
    12301 cttcgcggca gtagtccagg aagtttttaa ctgtttcagg tgctttatcg tcaaaagttt
    12361 tgatgacaat atcgccgtga ttggtgtgga aagtaaccat ttttgcatcc tgttccgttt
    12421 gattggtgct tcaacccagt tcgggtcata tatagggtgg tgttatagca taaccgcacg
    12481 atcggatcat cacgcaatgt atgctgattc gcgcgggaaa tatgggtatt atacgcaact
    12541 caattaccca cacatgtcta aacggaatct tcgatgctaa aaatcttcaa tactctgaca
    12601 cgccaaaaag aggaatttaa gcctattcac gccggggaag tcggcatgta cgtgtgtgga
    12661 atcaccgttt acgatctctg tcatatcggt cacgggcgta cctttgttgc ttttgacgtg
    12721 gttgcgcgct atctgcgttt cctcggctat aaactgaagt atgtgcgcaa cattaccgat
    12781 atcgacgaca aaatcatcaa acgcgccaat gaaaatggcg aaagctttgt ggcgctggtg
    12841 gatcgcatga tcgccgaaat gcacaaagat tttgatgctt tgaacattct gcgcccggat
    12901 atggagccgc gcgcgacgca ccatatcgca gaaattattg aactcactga acaactgatc
    12961 gccaaaggtc acgcttatgt ggcggacaac ggcgacgtga tgttcgacgt cccgaccgat
    13021 ccaacttatg gcgtgctgtc gcgtcaggat ctcgaccagc tgcaggctgg cgcgcgcgtt
    13081 gacgtggttg atgacaaacg caacccgatg gacttcgttc tgtggaagat gtcgaaagag
    13141 ggcgaaccga gctggccgtc tccgtggggc gcgggtcgtc ctggctggca cattgaatgt
    13201 tcggccatga actgcaagca gctgggtaac cactttgata tccacggcgg cggttcagac
    13261 ctgatgttcc cgcaccacga gaacgaaatc gcgcagtcca cctgtgccca tgatggtcag
    13321 tatgtgaact actggatgca ctccggaatg gtgatggttg accgcgagaa gatgtccaaa
    13381 tcgctgggta acttctttac cgtgcgcgat gtgctgaaat actacgacgc ggaaaccgtg
    13441 cgttacttcc tgatgtcggg ccactatcgc agccagctga actacagcga agagaacctg
    13501 aagcaggcgc gtgcggcgct ggagcgcctc tacactgcgc tgcgcggcac agataaaacc
    13561 gttgcgcctg ccggtggcga agcgtttgaa gcgcgcttta ttgaagcgat ggacgacgat
    13621 ttcaacaccc cggaagccta ttccgtgctg tttgatatgg cgcgtgaagt gaaccgtctg
    13681 aaagcagaag atatggcagc ggcgaatgca atggcatctc acctgcgtaa actttccgct
    13741 gtattgggcc tgctggagca agaaccggaa gcgttcctgc aaagcggcgc gcaggcagac
    13801 gacagcgaag tggctgagat tgaagcgtta attcaacagc gtctggatgc ccgtaaagcg
    13861 aaagactggg cggcggcaga tgcggcacgt gaccgtctta acgagatggg gatcgtgctg
    13921 gaagatggcc cgcaagggac cacctggcgt cgtaagtaat tgcgcaattg ccggatgcga
    13981 gttttcgcat ccggttatcg tctgcgccac cacaacattc ccatcagtag catccccggc
    14041 aaccacaccc acatcaattc ggaaataatc acctgatgcc cgtacgctgt ggtgtaacga
    14101 gacaatgcaa acggcgcgac tttaatcacc cgccagggag cgaaaaagcg ttcatctgac
    14161 cacggccaca gccagccaac gcccttaccg ccagtggtta ccgaatccag taagctgtgc
    14221 gatagcaacg agacggttaa aaacagccag cagcgaatca gcccagccct gaaccatcga
    14281 cgtccaatca gcacacataa gagcgggaca acaaacgcaa acaccagcga atgggtaaac
    14341 ccgcgatgac caaaaacatt gccgtaagca acgccaaatt taaacgacaa tacgtcggca
    14401 tcgggcagca tcgccaggat gattccggca aataacagac gcggagggat gactttcgaa
    14461 cccaacccta aaccaatgca aaggggaacg gcggcgtgcg taataacggt tggcatgatg
    14521 gtcgcttcgg caaaatgtcg atgctatcag catggatgaa cggggcgtag agggcaaaag
    14581 tctgaaaaga gaaccggcct gttgatacag gccgggaaag ggatcaggca acaacctgta
    14641 cgctgtgtcc tgcaaaactc actgtctgac cggcgacgat tttgcagcgt ttgcgcgttt
    14701 caaccgcacc gtcgactttc acctggcctt cggcaatcgc gattttcgcc tgcgcgccgc
    14761 tttcgctcca gccttccagt ttcagcaagt cgcacagctc aacgtgcgga tgtttaccta
    14821 aagaaaatgt cgccatgtta ctcaccctgt ggatcatgat attcaacgca cgcctgtagc
    14881 gtgttttcaa tcagcg
//
LOCUS       DBKEKI010000091        14857 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.091, whole genome shotgun sequence.
ACCESSION   DBKEKI010000091 DBKEKI010000000
VERSION     DBKEKI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.091"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..586
                     /locus_tag="ACUQPX_002828"
     CDS             <1..586
                     /locus_tag="ACUQPX_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HHV9057488.1"
                     /translation="CNLERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTA
                     YASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVC
                     DNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLP
                     WRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(583..1719)
                     /locus_tag="ACUQPX_002829"
     CDS             complement(583..1719)
                     /locus_tag="ACUQPX_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HHV9057489.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRLGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1725..2048)
                     /locus_tag="ACUQPX_002830"
                     /pseudo
     CDS             complement(1725..2048)
                     /locus_tag="ACUQPX_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
     gene            2231..2374
                     /locus_tag="ACUQPX_002831"
     CDS             2231..2374
                     /locus_tag="ACUQPX_002831"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057490.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2593..4131)
                     /gene="aldB"
                     /locus_tag="ACUQPX_002832"
     CDS             complement(2593..4131)
                     /gene="aldB"
                     /locus_tag="ACUQPX_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HHV9057491.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4296..5441)
                     /gene="yiaY"
                     /locus_tag="ACUQPX_002833"
     CDS             complement(4296..5441)
                     /gene="yiaY"
                     /locus_tag="ACUQPX_002833"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HHV9057492.1"
                     /translation="MLWHLQLSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTKLG
                     MAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHDCA
                     KGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNKMA
                     IVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALKAV
                     TMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLPHG
                     VCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKVDI
                     PAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            complement(5626..7470)
                     /gene="selB"
                     /locus_tag="ACUQPX_002834"
     CDS             complement(5626..7470)
                     /gene="selB"
                     /locus_tag="ACUQPX_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HHV9057493.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLGIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7467..8858)
                     /gene="selA"
                     /locus_tag="ACUQPX_002835"
     CDS             complement(7467..8858)
                     /gene="selA"
                     /locus_tag="ACUQPX_002835"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HHV9057494.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQCLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8956..9564)
                     /locus_tag="ACUQPX_002836"
     CDS             complement(8956..9564)
                     /locus_tag="ACUQPX_002836"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HHV9057495.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVLVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFACTKPPKA"
     gene            complement(9635..10720)
                     /locus_tag="ACUQPX_002837"
     CDS             complement(9635..10720)
                     /locus_tag="ACUQPX_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HHV9057496.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHLYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10723..11085)
                     /locus_tag="ACUQPX_002838"
     CDS             complement(10723..11085)
                     /locus_tag="ACUQPX_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HHV9057497.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11622..12311
                     /gene="mtlA"
                     /locus_tag="ACUQPX_002839"
                     /pseudo
     CDS             11622..12311
                     /gene="mtlA"
                     /locus_tag="ACUQPX_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
     gene            12307..13185
                     /locus_tag="ACUQPX_002840"
                     /pseudo
     CDS             12307..13185
                     /locus_tag="ACUQPX_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
     gene            13185..13772
                     /gene="mtlR"
                     /locus_tag="ACUQPX_002841"
     CDS             13185..13772
                     /gene="mtlR"
                     /locus_tag="ACUQPX_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HHV9057498.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13784..13993)
                     /gene="yibT"
                     /locus_tag="ACUQPX_002842"
     CDS             complement(13784..13993)
                     /gene="yibT"
                     /locus_tag="ACUQPX_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HHV9057499.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            14277..14639
                     /locus_tag="ACUQPX_002843"
     CDS             14277..14639
                     /locus_tag="ACUQPX_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HHV9057500.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LRREMGLEEMTGFSKTTF"
BASE COUNT         3670 a         3973 c         3650 g         3564 t
ORIGIN      
        1 ctgcaatctt gagcgtaatg ccttttatga aggcgtcgaa tttcctgatg attgtgatgt
       61 tttaatcagc tacgctccac caaaagatga atcgctggtt gccagtttta ttacacaata
      121 tgccgtaacc gcttatgcca gccagcgcta tcttgagaaa catcctatta gccgtcctga
      181 cgaactcgaa catcactcct gtattttgat cgactcgatg atgatcgacg acgcgaatat
      241 ctggcgcttc aacgtcgccg ggagtaaaga ggtgcgggat tatcgcgtga agggaaatta
      301 cgtttgcgat aacacgcaat cggcgctgga gctggcacga aatcacctgg ggattgtgtt
      361 tgcgccagac aaaagtgtgc agagcgatct gcaagacggc acgctggtgc cctgctttca
      421 gcacccgtat gaatggtggc tggacctggt ggctatcttc cgtaaacggg aatacctgcc
      481 ctggcgggtc cagtatgtgc tggatgaaat gctgcgtgaa attcgccacc agcttgctca
      541 gtcgcagcaa ctgcggcctg aacaagcggc ggaaagcgaa gattaatgat cgagataaag
      601 gtaatgcacc cagctggtca tgcgtaatag cactttgcgc atcaccgaga catggctgaa
      661 atgatcagag tagaccgcca cctgggcgta aataccgtct ggtaaagcgc tcaaatcagt
      721 gtgctcatcc agttcaatgg tcgcgataac gccatctgaa cccggcgctg tgtttaacgt
      781 ctgtaaggtg ccggtcgact gataagctcc gccgggaaca gctggactaa tggctgccag
      841 cttgccgctg aataccttac ctggcagagc attaaacacc acttccgcat cgtcgccagg
      901 ggccagtcgc agcaaggagt tctgacggaa ctgcgccaca atttgtcgtt tctgatcggg
      961 tataaacacc atcaccggac gtagcggcag cgacgcggca taggtaccca gacgaatcag
     1021 cacctgggtc acataaccat cgctcggcgc acgcactatc gtctgctcaa ggttatattt
     1081 tgcttccgcg agctgcgctt tcaggctggc gatttgagaa tgttcaccca acaccaggct
     1141 atccagctgg ctctggatct gtttttgttc cgccgccgat gactttacgg aggcttcctg
     1201 cgccagataa ttttgccgcg ccacatcaat atcgcgttct gaaaacgggt ttactttcgc
     1261 ctggctgccg cgtgcgtaac gctgatactc tttagcgaat ttatcccgcg tggcctttgc
     1321 ctgctgagta ttcgccgcca tctcatctaa ctctgcgccc aacgcccgct gtttatgttc
     1381 tgcggtaacg atatccgcca tcagccgatc cacccgcgcc tgataacgcg tcgggtccag
     1441 tcgaaatagc acctcacctt ttttaatcag cgtatttttc ttatccgtca cttcgatcac
     1501 cacgcctgtc acctgtggga caacaggaat agaaataacc gctttttgcg ctttgaaggt
     1561 atacggatgg ttatagttca ttaacagaat taaaccactg acaataaata tgccacccag
     1621 ggccgctgtg gaaatggtcc atttatttac aggaattttg aagatcttaa acattgccca
     1681 tgcaaaagcc acataggtca aaataatcag taaatccata agtattactc ggcagaagag
     1741 gatttgatgt cggcgagctg tttctccagc ccggcaatac gctgttgtag ttgcacaacg
     1801 gaggagtcat ggttttacat tccccatccc cgctccgggc ggtaaagcgt ggcccaaatc
     1861 cacaaaaatg gccagataac atgcagcgta aaaagactca cccaaccagc aacatgaatg
     1921 gcgtcggcat gaggatgatt acgtttttta gcaatcaggt agggaatatc atgtaaaata
     1981 ataatcccat agaatatcac cagaaataca aaaataagca ctcccagtgc aaaatagtcc
     2041 aggaacatat ttccctcaaa gaatataaaa aagaacaatt aacgcatatt atgaaatgcc
     2101 atgatgcagt gcaatttcct tatttatata acgacatatt gttttttata tactttctga
     2161 aatgtgctgt ctggtttttc aacaggacga ggtttatcat attgaaatag tggaactatt
     2221 agccaaacta atgaataact ccagtaaaac atccacagta cagattaagc gtattaaacc
     2281 ttcaattatc taccgtttat tgctgattgg cctcggatca ccaatggtga tttacggcct
     2341 ggttcgcccg ctcaccatcg aaacgcgaga ttaaactcct gacaaaacac gccccagaaa
     2401 cgcacttctg ttggcgtagt ctgggttatt gcgcacgtag gtttaaaacg taaattgtaa
     2461 caacgtgcgc tttgtttatg ccggatgcgg cgtgtacgcc ttatccggcc tacggtcagg
     2521 ttcccgtagg catgataaga cgcgtaagcg tcgcatcagg caatgaatac ccaatgcgac
     2581 cagcttctta tatcagaaca gccccaacgg tttatccgag tagctcacca gcaggcactt
     2641 ggtttgctgg taatgctcca gcatcatctt gtgggtttcg cgaccgatac ctgattgttt
     2701 gtagccacca aacgcagcat gtgccgggta agcgtgataa cagttggtcc acacgcgccc
     2761 agcctgtatg ccgcgcccca tcttataggc cagattaccg ttgcggctcc agacgcccgc
     2821 gcccaggcca tattgcgtat cgttcgccag ctccagcgct tcttccatcg ttttgaaggt
     2881 ggtcaccgcc agcaccgggc caaaaatctc ctcctggaac acccgcatat tgttctgacc
     2941 aaacagaatc gtcggttcga ggtagtagcc gtctttcagt tcaccttcca gcagcttgcg
     3001 ccgcccgcct gtgagcacgt cagcgccctc ttttttaccg atatcaatgt agttgaggat
     3061 ggtttccagt tgcccgtgag aaacctgcgc gcccatttgc gtcacgctgt cgagcgggtt
     3121 accgctgcga atgctttcga cacggcggat ggcgcgttcc ataaagcgtt cgtagattga
     3181 ctcctgcact aaggcacgac tcggacaggt gcaaacttcg ccctggttaa aggcaaacag
     3241 tgcaaagcct tccagcgctt tatcgaaaaa ggcatcttct tcatccatca catcagcaaa
     3301 gaagatattt ggcgacttac cgcccaactc cagcgtcacc ggaataatgt tttgcgttgc
     3361 gtattgcata atttgttggc ccacttccgt tgagccggta aacgccactt tggcgatgcg
     3421 tttcgaggtc gccagatatt cgccaatttc cccacctgcg ccattgacca cgttcaccac
     3481 gcccggcggc agtaaatcac cgacaatttc cattagcagc agtacagaaa gcggggtaag
     3541 acgtgcgggt ttcagcacca cacagttgcc cgccgccagc gcgggagcca ttttccagct
     3601 cgccatcagc agcgggaagt tccacgggat aatctgcccc accacgccta acggttcatg
     3661 gaaatgatag gccacggttt cgctatcaac ttcactgatc ccaccttcct gcgcccgaat
     3721 acacgaggcg aaatagcgga aatggtcaat cgccagcggt acatccgcag cactggtttc
     3781 gcgaatgggt ttgccgttat cccaggtttc agctgtcgct aacagctcga ggttttgttc
     3841 cattcgatcg gcaatcttaa acagaatcgc cgcgcgatcc tgcaccgagg tgtgcgccca
     3901 tttatctttc actttgtgcg cagcatccag cgccagatcg atgtctcgtt tgcccgaaga
     3961 cgccacttcg cacagcagct gcccggtcac cggcgtcagg ttctggtaat actcaccatc
     4021 ggcaggagct acccattcgc cgccaataaa gttgtcgtag cgggctttta acttgatggg
     4081 gaaaccatac tcgccgggct taatctgtgc tgaaggggga ttattggtca tgatcgtctc
     4141 cttgcggtgt gaggtataac aagggtagac gtgactggcg aaatcttcgc cagtcggtaa
     4201 cagctttacg acagctatca cgaatttacg ggcaaggatt acagagatag ttgaaaaagc
     4261 ggctgacaat ttgccagccg ttgttggaat gatgattaca ttgcggcgcg ataaatcgcc
     4321 acaatttctt cgtgagttgc ctggatcggg ttagtaaagc cacaggcatc tttcagggca
     4381 ttagtcgcca gaaccgcgaa atcttcttct ttcacgttca ggtcgcgtag gcctgccggg
     4441 atatccactt tcttcgccag ttcacggatg gcgttaatgc aggcttcagc accttccgcg
     4501 tcgtttttac ctgtcacgtt cacgcccatt gcagcggcac agtcacgcag acgtgcagcg
     4561 gcgactttgc tgttgaatac ctgaacatgc ggcagcagaa cggcgttaca tacaccgtgt
     4621 ggcaggttgt agaaaccgcc cagctggtgc gccatcgcat gaacataacc cagagaagca
     4681 ttattgaacg ccataccggc aaggaactgg gcataagcca ttgcttcacg cgctttcgca
     4741 ttactgccat cttcaacggc taacggcagg ttttcggcaa tcatggtcac ggctttcagt
     4801 gcacaagcgt cagtgatcgg cgtggcggca atagaaacat acgcttcgat agcgtgcgtt
     4861 aaggcatcca tacccgttgc ggcggtcagt gacttcggca taccaatcat cagagaggag
     4921 tcattgacag aaagcagcgg agtcacgtgc ttatcaacaa tcgccatttt gttatgacgc
     4981 gcttcgtcag tgatgatgca gaaacgggtc atttcagagg ccgtacccgc cgtggtattg
     5041 atggcgatca tcggcagctg cggttttgca gagcggtcaa cgccttcgta atcgcgaata
     5101 tcgccgccat tggctgccac cagcgcaata ccttttgcgc agtcgtgtgg agaaccaccg
     5161 cctaaggaga tcacgctatc gcaattattt tctttaagta atttcaaacc tgcggcgacg
     5221 ttttccgtgg tggggttagg ttgggtgcca tcataaataa cgctaaaaat attgcgttct
     5281 tccagtgctt tttgcacatc acccgccata cctaatttcg ttaacatatt gtcagtgaca
     5341 attaaggtac gggtaaatcc ataatctgcc atcatattca ttgcatcagt caatgaatca
     5401 gcgccgatga cattcactga aagttgaaga tgccataaca cactcctgaa agtgtaaaaa
     5461 agggtgggca gcttactgag gattttcatt cgaaaacatg atcattatta ttaattatca
     5521 aaattaatct aataaaaagc acattgtatt cattaaacaa tgtgctttat ttaaaatcat
     5581 aagaaatttg tattaattta ttttaaaaat taattaatca tttccttatt tttccggaaa
     5641 taataatgcg tcgcgcaata aatgatcatt tccacgacga cgcgtaaagc caatgcggtc
     5701 aaaatattcc agaatctgaa ttcccagctt tcggcctacg cctaagcgat cgcggaaatc
     5761 cgccgcgcag gttgaaccac actcctgatc gagatcgcgg atcatattgg caaattcgac
     5821 aatccgatcg ttacggtaat aacgatcttt gacgatcgcg gtaattattc cttgctgcgc
     5881 cgcctggcgt agcgtcaggc gcattgcctg ctcgtcggta cccgtctctt ttgccagatc
     5941 acgcacccac cacggctcgt caccaaacag cggctctgct ttttgccaga tggcctgctg
     6001 ctcttcgctg aagcccgctt tgtgatccgg cagatgcagc caaccgtgat gactgaggat
     6061 gtcgccgctt tcgcgcatct tttcaatcag caacagtact agcgcttcat cttccattgg
     6121 caacgccata cgtcgcagac gttcgcgccc aggcccaggt tcatcgcgat gttgctcatg
     6181 ataagtcgct aatgtgtcga gaattttccg ttgccagcga gcggcaaccg gcgcattcaa
     6241 caagctataa ccagcctgaa tatagccagg ctgttgcagc agttcgcgca tcccttcgcc
     6301 gttgagctgg cgcgcccagg cgaaatcagg aagattaacc gcgccgcgtt gcaggtgaac
     6361 ggctaacgct tcgctatcat tctgtgccct ggcgagggaa gccagccatt gcagatactc
     6421 tggtttacgt ttaccgcgac gcggcgggtt aagcatcacg acgcgcgctc cggccagcgt
     6481 gttgcgggca gagatatcgc gcaataccag gcggtcgtta tctgccagcc ataacggggt
     6541 gtcgaagacc agttcagcaa ggttatcttc cagcagtgaa acgcgtcccg tgacgtggct
     6601 ggcggcgtgg tgaatatgca gcggctgcca ctgggtcagc ggtgtatggg tttgaagctc
     6661 gacaatcacc cgtgtgaacg gctctggcgg cacatcggca agcagccagt cgccacggtt
     6721 aatctgctct ttttccgcat cacccgcgat gtcaagcgcg atacgctgcc cagcattggc
     6781 ggtttctgtt ggctggtttt gcgcatgcag cgcacgtaca cgcatcggtt tatttacacc
     6841 agtcagccag agtgaatcgc ctaccttcac ttccccgctt aacgccgtac cggtgacgac
     6901 cagcccggca ccttttacgg taaatgcgcg gtcaatcgcg aggcggaaac tatgttggct
     6961 ggcgtgctcg cgttccggca actgaagcag atgctcgcgc agggcatcaa ttccccgacc
     7021 ttcggttgct gcggtgataa acagttttgc ctcagcaaaa ccgtattccc gcagaacctc
     7081 ctttacctgg cgttcaacct catcaacacg cgcttcgtcc acgcgatcgg ctttggtcag
     7141 cgccactgtc agcatcgggt taccggtcag ctgcaaaatc gccagatgct cacgggtctg
     7201 tgccatcacg ccgtcatcgc acgccaccac caacagcgcg tgatcgatac caccaacgcc
     7261 cgccagcatg ttggaaagaa acttttcatg accgggaacg tcgataaaac caggcacgcg
     7321 gccatccggc tgcggccagt aggcatagcc gagatcgatg gtcatgccgc gctttttttc
     7381 ttccggcaga cggtcagcat ttacgccagt aatcgcctgc aataaggttg ttttgccgtg
     7441 gtcaacgtgt ccggcagtcg caataatcat ttcaacaaca tctccaaaaa ccgttgctca
     7501 tcttcaaggc agcgtaaatc cagccacaat cgtccgtcat aaatacgacc aatcaccggc
     7561 actggcaatt cacgccagcg ggcggctaat gactcaaggt ggctaccgcg tccatcatgg
     7621 ggtgtaaacg ttaatgccgc gctcggcagg cgatcaaccg gcagcgaacc actgccaatc
     7681 tgcgaaagac atggcataac ctgtaccgca aactccgcgc cgtaatgtgc ggcaagggga
     7741 gcctgtaaac attgtgcctg gatttgaatg acctctgcgc tgcgggtaag caggcgcagg
     7801 gtcggtaatt tttcactcag agcttcaggg tgtaaataaa gacgcaacgt ggcttccagc
     7861 gccgcgaggg tcattttatc cgcgcgtaat gcacgcttca gcgggtggct ttgcaggcgg
     7921 gcgatcatct cttttttacc aacgataatc cctgcctgcg gcccgcctaa caacttgtcg
     7981 ccggagaagc tcaccagact gacgcccgcc gcaatcaact cctgcggcat tggctctttc
     8041 ggcaaaccgt actggctaag atcgaccagc gagccactgc ctaaatcagt caccactgga
     8101 acatccagct ctttgccgag cgccaccagt tcagcttcgt ctatcgcttt ggtgaacccc
     8161 tgaatgctgt agttactggt atgtactttc atcaacagtg cggtattttc attcaccgcc
     8221 tgacgataat cattcgcgtg cgtgcggttg gtggtcccta cttcgtgtag ggtacagcct
     8281 gcctgacgca taacatcggg aatacgaaac gcgccgccaa tctccaccag ttcgccgcga
     8341 gataccacca cctctttccc gctggcagtg gccgccaaca tcaataacac cgccgccgca
     8401 ttgttattga cgatacaggc atcttccgcc cccgtaatac ggcacagcag ctgcgccagc
     8461 gcccgatcgc gatgtccgcg tccggcgtcg tccagatcgt actcgagggt cactggcgaa
     8521 cgcatagcct gcgcaacggc ttccaccgct gcttccgcct gtaaagctcg cccaaggttg
     8581 gtatgcagca cggttcccgt caggttgatc accggacgca gcgcgctctg cgcctctttc
     8641 gtcaaccggg catcgacttc ttgcgcccag ttttcacacc acgcaggcag cgtctggctg
     8701 ccacgaatca cttctcgcgc ttcgtcgagc atctgacgca acaattccac cacgcgggtg
     8761 tgaccataag tatcacgcaa agaaaggaag gagctatcgc gcaataagcg atcaatagcc
     8821 ggaagttgac tatagaggga acgcgtttcg gttgtcatag gaaacctggc tgatcaaggc
     8881 cctctcacac ggagaagggc gtttaacata accacggatt gtaacgtgag atgggtcagg
     8941 aggacatatc gcgcatcaag cctttggcgg tttggtgcaa gcaaaacttt cgcggctaaa
     9001 caggttttcg accagtttga ctaagtgtgg gcggtcgaca caccaacctg gcgcaacgcg
     9061 gcggaaattg agatatccga cagcacaggc aatggcgata gtcgccagat tgaccgtatc
     9121 ggttttgagt gtgccatcga ccagatatcc ttccagcaca tccagactgc ggttgatttt
     9181 ctcccgctgg cgtaacaatt catcttcaga ctgctgcgcc gccggacgcg cctgttcacg
     9241 caccgatacc agcccggcat ccataatgcc atccgccagc gcctcaattt tgcgcacccg
     9301 caacgactcc agcggatcgc gcggcaacat cgccggagcg acattcatta actcaatata
     9361 ttcagcgatg atcggcgaat caaaccagca ttcgccctct tcggtcacca gcaccagcac
     9421 tttccctaac gggttaaatt gcgccacgcc gttatccgcg ttatagggca gttcattaat
     9481 aaattcgaaa gttatgccct tttctaacaa cagaatagaa agtttgcgta caaacgggct
     9541 ggtgtagcta ccgacgagtt tcatgccgag tcctttgtgc gaggaaaaat atcagtatgg
     9601 ctcaggctca cgcaaaacac aggtgatata agtctcaatg atccaaataa agataatgca
     9661 tccaactggt cattcttagc agcactttcc gcatcaccga aacatggctg aaatggtcgg
     9721 agtaaaccgc cacctgggcg tagatgccgt cgggtaaggc atcgatatca tcgttagggt
     9781 ccagttcaat ggttcccagc acaccgtccg tgccgggaac gaccgttaat gattgcaata
     9841 ccccctgcgc ctgataagaa ccgcctggca cgacaggtaa aatactagtc aatttgccgt
     9901 ggaacacctg cccaggtagc gcgttaaaca ccacttccgc atcatcgcca ggtttcagac
     9961 gtaacagcga gttttgccga aattgggcga caatttgccg tttttgctcg gggatgaaga
    10021 ccatcaccgg acgcagcggc aaggcagctg cgtatgtacc tgggcggatc agtacctgag
    10081 tgacgtagcc attgcttggt gcgcgaatga cagtctgctc aagattatat tttgcttcag
    10141 taagttgcgc tcttaagctc acaatctgcg attgctcgcc gttgaccata ctgtcgagct
    10201 gactctggat ctgcgcctgc tccgccaccg agcctttcac taacgcatcc tgcgcgagga
    10261 aattttgccg cgcatcgtcg atgtcacgtt ccgagaacgg attcaccgcc gcctggctgc
    10321 ctttcaggta acgttgataa tttttaaaca gacggtcgcg ctccgctgaa acctgggtgg
    10381 tgttggcctg cgcttcagtg agctgcgcac gcagcgtctt tatattatgc gtcgccgtca
    10441 tcaggtcagc ctgaagtctg tcaactcgcg cctggtaacg aaccgggtcg agcttaaaaa
    10501 gcacctcgcc cttttgaata agctgattat tcttgtcagt gacttcagta acaattcccg
    10561 tcacctgtgg cgtgataggg atcgctatca ctgccttttg cgcggtaaaa gtgtaaaggt
    10621 ggttgtagtt catcaacaaa atcaaaccac tcaccagaaa cacgcctccc agcgccgccg
    10681 tcgccagcgc cacgtaagtt aaaacaatca atagatccat aattagatct ccgggaacgt
    10741 ggaatagtca gtctttttct cggcggcgag ttgatgctcc agccgggaaa ttcggtcaga
    10801 aagtgcggcg atttccggtt cagtcgcttt ctcctgcgac gcaacatgtg actgcatacc
    10861 ccagccacgc tccggttgat agagcgtcgc ccagatccac agaaacggcc agataacatg
    10921 cagagtaaac aggctcaccc agcccgccgt atgaatagcg tcggcatggg ggtgattgcg
    10981 ctttttggca atcagataag ggatgtcatg tatcgcgatg attccataaa aaatcaccag
    11041 aaagacgaag atcagcactc ccaacgcgaa atagtttaga aacatatctg cctcggattc
    11101 acgtttatca gtgttgtttt ttggctggca gccagaaggg agtcaggctg atattttgac
    11161 aataatccgg gttcgcgatt ctcgccataa caccaaagaa taatttttag aggtgatgag
    11221 ttgcttagtt acataacgat tgtatgacga aggcataaca tgctgtagat cacatcaggt
    11281 gaacgccgta agaaaatatc ttgtgattca gatcacaaag attcaacaaa ccatcaaaac
    11341 aaaaatgtga cattactcac atttaaatgc catttttagc gaaaatcacc gccttgtttc
    11401 ttttttacac aggcgttttg tgatgagcgt cacgtcaatt acctctctac cccctatatt
    11461 tatgtgactg atatcacaca aaagcccgtc gactggacag ttaaccgatt cagtgccaga
    11521 tttcgcagtc tctacaaggt ccggctacct ctgccgccac attaacaaaa aacctcgggc
    11581 ttccagcctg cgcgacagca aacataagaa ggggtgtttt tatgtcatcc gatattaaga
    11641 tcaaagtgca aagctttggt cgtttcctca gcaacatggt gatgccaaat atcggcgcgt
    11701 ttatcgcgtg gggtatcatc accgcgttat ttattccaac agggtggtta ccgaacgaga
    11761 cgctggcgaa gctggtcggg ccgatgatca cttatctcct accgctgctg atcggttata
    11821 ccggtggtaa gctggtaggc ggcgaacgtg gcggcgtagt cggtgccatt accactatgg
    11881 gcgttatcgt cggcgcagac atgccgatgt tcctcggttc tatgattgca ggtccgctgg
    11941 gcggctggtg cattaagcac ttcgaccgct gggtagacgg taagatcaaa tccggttttg
    12001 agatgctggt gaataacttc tccgcaggca tcatcgggat gatcctcgct attctggcat
    12061 tcctcggcat tggcccgatt gttgaagccc tgtccaaaat gctggctgcg ggcgttaact
    12121 tcatggttgt ccatgacatg ctgccgctgg cgtctatctt tgttgaaccg gcgaaaatcc
    12181 tgttcctcaa caacgccatt aaccacggta tcttctcgcc gctgggtatt cagcagtccc
    12241 atgaactggg taaatcaatc ttcttcctga ttgaagctaa cccaggtcca ggtatgggcg
    12301 tgctttgtgc tggaacgtat tgctccggct atcgccaaag ggctggtgaa acgcaaagaa
    12361 caaggtaatg aatccccgct gaacatcatc gcctgtgaaa acatggtacg cggcaccacg
    12421 cagctgaaag gtcatgtgat gaacgccctg ccggaagacg ccaaagcgtg ggtagaagag
    12481 cacgttggct ttgtcgattc cgccgttgac cgcatcgtac cgccttcggc ttcggcaact
    12541 aacgatccgc tggaagtgac ggtagaaact ttcagcgaat ggattgtcga taaaacgcag
    12601 ttcaaaggcg cactgccgaa catcccaggc atggagttaa ccgacaacct gatggcattt
    12661 gtcgaacgta aactcttcac cctgaacacg ggtcatgcta taaccgcgta cctcggaaaa
    12721 ctggccggtc atcagaccat tcgtgacgct attctcgacg agaaaatccg cgcggtggta
    12781 aaaggtgcga tggaagaaag tggtgcggta ctgatcaagc gctacggctt tgacgcagac
    12841 aaacatgcgg cgtacatcca gaaaatcctc ggtcgttttg agaacccgta tctgaaagat
    12901 gatgtagagc gcgtaggccg tcagccgctg cgtaaactga gtgctggcga ccgtctgatc
    12961 aagccactgc tcggtacgct ggaatacagc cttccgcaca agaatctgat tcaggggatt
    13021 gctggtgcaa tgcacttccg cagtgaagat gatccgcagg ctcaggaact ggcagcactg
    13081 atcgctgaca aaggtccgca ggcggcgctg gcacagattt ccggtcttga tgccaacagc
    13141 gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc aataatggtg gaccaggcgc
    13201 aggacaccct gcgcccgaat aacagattgt cagatatgca ggcaacaatg gaacaaaccc
    13261 aggcctttga aaaccgtgtg cttgagcgtc tgaatgctgg caaaaccgtg cgaagctttc
    13321 tgatcaccgc cgtcgagctt ttgaccgagg cggtaaatct tctggtgctt caggtattcc
    13381 gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt actcgacggc gacggtccgc
    13441 tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt gggcgtcatt aaccgccagg
    13501 aatacgaaga tgcggaactg ctgatggcat tgcgtgaaga gctaaatcac gacggcaacg
    13561 agtacgcctt taccgacgac gaaatccttg gaccctttgg tgaactgcac tgcgtggcgg
    13621 cgttaccacc gccgccacag tttgaaccag cagactccag tttgtatgca atgcaaattc
    13681 agcgctatca acaggctgtg cgatcaacaa tggtcctttc actgactgag ctgatttcca
    13741 aaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc tgcttactgc ccctctacct
    13801 gcttcggccg ataaagccga cgataatact ccagacgttg tagatataac ggcacgcttt
    13861 catcgggtat tccggacgga atcgcgttac ggggaggaag ttttttcaga tactcccgga
    13921 acgcctggct tgatgccatg aaatctacgg ctttatcgat aagaagcgga acgttttcac
    13981 ctaatttgcc catgatgata tctccgtatt accccgcgcc gggaatgcgc ggccgccaat
    14041 tttagtttag gttcccggta aagatgacat taaggaaaac gtgctgaatc ctcaataatc
    14101 atccgctgct gtttattatc tttttctata gatttcttaa ttaatcaacg aattgcgtag
    14161 tttttgagca tgttaatgat tacgttgcag aactattaca atgtcatcag ttgtgaacaa
    14221 agcacctggt cgcgcatact aggggctata aatttatctt tatcagaagc catcacatga
    14281 aagaagtcga aaaaaacgaa atcaaacgtc tcagcgatcg cctggacgcc atccgccacc
    14341 agcaggccga tctgtcgctg gttgaagccg cagacaaata tgccgagctg gaaaaagaga
    14401 aagccacgct ggaagcagaa attgctcgcc tgcgtgaggt tcatagccaa aaactgagta
    14461 aagaagcaca gaagctgatg aagatgccgt tccagcgcgc aattaccaaa aaagagcagg
    14521 ctgatatggg caagctgaag aaaagtgttc gcggactggt ggttgtgcac ccaatgaccg
    14581 cgctgaggcg cgaaatgggc ctggaagaga tgactggatt ttctaagact acgttttaag
    14641 cccctcagac gactggttcg atcgtatcaa cccgcatctg gcttgcctta gctggtcaat
    14701 gcggggactc tcctgtctac tcgcctaatt accaaacaat tcactccctt tacttcgatt
    14761 taccgctggt tgatattctg aaaatgcatt tttaccgaaa tagccatcta taaacaataa
    14821 aaatatatag aagatatcat ttgttaatta acaagca
//
LOCUS       DBKEKI010000092        14648 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.092, whole genome shotgun sequence.
ACCESSION   DBKEKI010000092 DBKEKI010000000
VERSION     DBKEKI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.092"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..954)
                     /gene="istA"
                     /locus_tag="ACUQPX_002844"
     CDS             complement(<1..954)
                     /gene="istA"
                     /locus_tag="ACUQPX_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HHV9057501.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLV"
     gene            complement(1855..2352)
                     /locus_tag="ACUQPX_002845"
     CDS             complement(1855..2352)
                     /locus_tag="ACUQPX_002845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057502.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(2381..3216)
                     /gene="aadA1"
                     /locus_tag="ACUQPX_002846"
                     /pseudo
     CDS             complement(2381..3216)
                     /gene="aadA1"
                     /locus_tag="ACUQPX_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            complement(3274..3798)
                     /gene="sat2"
                     /locus_tag="ACUQPX_002847"
     CDS             complement(3274..3798)
                     /gene="sat2"
                     /locus_tag="ACUQPX_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HHV9057503.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(3893..4366)
                     /gene="dfrA1"
                     /locus_tag="ACUQPX_002848"
     CDS             complement(3893..4366)
                     /gene="dfrA1"
                     /locus_tag="ACUQPX_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HHV9057504.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            4698..5675
                     /gene="intI2"
                     /locus_tag="ACUQPX_002849"
                     /pseudo
     CDS             4698..5675
                     /gene="intI2"
                     /locus_tag="ACUQPX_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(5863..6102)
                     /locus_tag="ACUQPX_002850"
     CDS             complement(5863..6102)
                     /locus_tag="ACUQPX_002850"
                     /inference="COORDINATES: protein motif:HMM:NF020425.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HHV9057505.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(7044..7475)
                     /locus_tag="ACUQPX_002851"
     CDS             complement(7044..7475)
                     /locus_tag="ACUQPX_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HHV9057506.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(7543..10254)
                     /locus_tag="ACUQPX_002852"
     CDS             complement(7543..10254)
                     /locus_tag="ACUQPX_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HHV9057507.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRIRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVELGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPRMGFEPEGSYQQFNDVFAEMVAWHNAAGITGSGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(10266..10598)
                     /gene="mobA"
                     /locus_tag="ACUQPX_002853"
     CDS             complement(10266..10598)
                     /gene="mobA"
                     /locus_tag="ACUQPX_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HHV9057508.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            10831..11166
                     /locus_tag="ACUQPX_002854"
     CDS             10831..11166
                     /locus_tag="ACUQPX_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HHV9057509.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRNTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(11252..12100)
                     /locus_tag="ACUQPX_002855"
     CDS             complement(11252..12100)
                     /locus_tag="ACUQPX_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HHV9057510.1"
                     /translation="MNQALPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKRNIIRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            12679..12930
                     /locus_tag="ACUQPX_002856"
     CDS             12679..12930
                     /locus_tag="ACUQPX_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HHV9057511.1"
                     /translation="MTGWELRIWRKSMLWSREKASREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(12961..13230)
                     /locus_tag="ACUQPX_002857"
     CDS             complement(12961..13230)
                     /locus_tag="ACUQPX_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057512.1"
                     /translation="MRTTKPGKIPGFFVSRSRANSMTIAERLIQKGFDEGFKEGFKQG
                     FKQGVLEVAREIACRLRDIGWPPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(13230..14168)
                     /locus_tag="ACUQPX_002858"
     CDS             complement(13230..14168)
                     /locus_tag="ACUQPX_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HHV9057513.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGRMEGAL
                     EKALAIACQLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(14165..14554)
                     /locus_tag="ACUQPX_002859"
     CDS             complement(14165..14554)
                     /locus_tag="ACUQPX_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HHV9057514.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
BASE COUNT         3683 a         3803 c         3327 g         3835 t
ORIGIN      
        1 caccagattt tcatcaagat gcacgtcata ctcttttttc tccggaggca gtgccagcat
       61 ggactgctgc tcttcgagcc agcgatcgca gggacgtgcc tggattgttt catgctttcg
      121 ttggttagcg acatcgtgca gccagcgcag accgtggcgg ttggctgttt caacatcgac
      181 agtgatcccc atcgggcgca ggcgagtcat tagtgggatg taaaaactgt tacgggtgta
      241 ctgcaccatc cgttccacct tacctttagt ctgtgccctg aaggggcgac acagtcgggg
      301 agagaagccc atctccttgc cgaactgcca cagcgaagga tggaaccggt gctgaccggt
      361 ctgatatgcg tcacgttgca gaaccacagt tttcatattg tcatacaaca cttcgcgcgg
      421 cacaccacca aagaagcgga acgcattacg atggcaggtc tccagcgtgt cataacgcat
      481 attgtcagtg aattcgatgt acagcattcg gctgtatccg agaacagcaa cgaacacgtg
      541 aagcggtgag cgaccattac gcatagtgcc ccagtcaacc tgcatctgtc gtccgggttc
      601 agtttcgaac cgaacggcag gctcctgctc ctgaggaacc gagagagaac gaatgaatgc
      661 cctgagaatg gtcattccgc cacgatatcc ctggtctctg atctcgcgag cgattaccgt
      721 tgccgggatt ttgtaaggat gagcatcggc gatgcgttga cgaatataat cccggtattc
      781 atctaggagt gaagcaacag caggtcgcgg cgtatatttt ggcggctcag attttgcctg
      841 caaataacgt ttaacggtat tgcgggagat ccccagttct ctggcaatcg cccggctact
      901 cattccctgc ttgtgcagga ttttaatttc cataactgtc tcaaaagtga ccataagctc
      961 tcctgaatca ggagagcaga ttaccccctg gatctgattt caggcgttgg gtgtggatca
     1021 ctattgcacc gttcgtgaca attaggggat tcatcagagc ggagcattag tcccaaagca
     1081 tctccggtag atccggaggc tccatactga aggcatggca tcttgggcat tgatatttga
     1141 aacttctctc tagatagcag ggatttcttg gccttgcctc atgctgatag cttgtaaata
     1201 tcacacgatc cttagcccca caaagtgagc atactccgta gctttcattt atagaaatcg
     1261 tcgttagatt gtcgcagcat gtgcacgttc cgactatttg ctcaggaagc atcgccatac
     1321 cttggcctag gtaaagaaga tcagactcgt agccacatcg gtggcaactg agcttgtacg
     1381 caatgcccat ctggttgtct cacctgaatc gtaacgtcag agctattcat ccatccattc
     1441 ggcatcaatg tatgccacta agccgaccag ctgaccgttt ggactgaagt atgttgcctg
     1501 caccccactt gacatctcat caataccacc gcactgcgct aggcgccggt cccacttgcc
     1561 cacagaagcg tcaggatcgg ctgagttccc caagaccctt gtgagccatg cctcctttaa
     1621 ctcaacctct cgaatgattg cgtcggccag ctcctccgcg ttatctagtt cgacatagtc
     1681 tggaattcgg acgtacgttc tgaacacagc tggatactta cttgggcgat tgtcatacat
     1741 gacatcaaca atgtacccgt ttgtgtaacc gtctcttgga ggttcgtatg acactagtgg
     1801 ttcccctcag cttgcgacta gatgttgagg cctaacattt tattagagag caggctagtt
     1861 gcttagatac atgatcttca ggccgttatc tgtcagggca agcgaaaatt ggccatttat
     1921 gacgaccaat gccccgcaga agctcccatc tttgccgcca tagacgccgc gccccccttt
     1981 tggggtgtag aacatccttt tgccagatgt ggaaaagaag ttcgttgtcc cattgttggc
     2041 aatgacgtag tagccggcga aagtgcgaga cccatttgcg ctatatataa gcctacgatt
     2101 tccgttgcga ctattgtcgt aattggatga actattatcg tagttgctct cagagttgtc
     2161 gtaatttgat ggactattgt cgtaattgct tatggagttg tcgtagttgc ttggagaaat
     2221 gtcgtagttg gatggggagt agtcataggg aagacgagct tcatccacta aaacaattgg
     2281 caggtcagca agtgcctgcc ccgatgccat cgcaagtacg aggcttagaa ccaccttcaa
     2341 cagatcgcgc atagtcttcc ccagctctct aacgcttgag ttaagccgcg ccgcgaagcg
     2401 gcgtcggctt gaacgaattg ttagacatta tttgccgact accttggtga tctcgccttt
     2461 cacgtagtga acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttgtcc
     2521 aagataagcc tgcctagctt caagtatgac gggctgatac tgggccggca ggcgctccat
     2581 tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat
     2641 gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca
     2701 tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag
     2761 ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat
     2821 agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg
     2881 ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg
     2941 cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt
     3001 ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt
     3061 aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa
     3121 atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag
     3181 ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaactcct gaattaagcc
     3241 gcgccgcgaa gcggtgtcgg cttgaatgaa ttgttaggcg tcatcctgtg ctcccgagaa
     3301 ccagtaccag tacatcgctg tttcgttcga gacttgaggt ctagttttat acgtgaacag
     3361 gtcaatgccg ccgagagtaa agccacattt tgcgtacaaa ttgcaggcag gtacattgtt
     3421 cgtttgtgtc tctaatcgta tgccaaggag ctgtctgctt agtgcccact ttttcgcaaa
     3481 ttcgatgaga ctgtgcgcga ctcctttgcc tcggtgcgtg tgcgacacaa caatgtgttc
     3541 gatagaggct agatcgttcc atgttgagtt gagttcaatc ttcccgacaa gctcttggtc
     3601 gatgaatgcg ccatagcaag cagagtcttc atcagagtca tcatccgaga tgtaatcctt
     3661 ccggtagggg ctcacacttc tggtagatag ttcaaagcct tggtcggata ggtgcacatc
     3721 gaacacttca cgaacaatga aatggttctc agcatccaat gtttccgcca cctgctcagg
     3781 gatcaccgaa atcttcatat gacgcctaac gcctggcaca gcggatcgca aacctggcgc
     3841 ggcttttggt acaaaaggcg tgacaggttt gcgaatccgt tgctgccact tgttaaccct
     3901 tttgccagat ttggtaacta taatttatgt tagaggcgaa gtcttgggta aaaactggcc
     3961 taaaattgct ggggatttca ggaaagtaaa catcaccttc cggctcgatg tctattgtag
     4021 atatatgtag tgtatctact tgatcgatca ggcttttgta tatctcccca ccacctgaaa
     4081 caatgacatg atccgttatt ttctttaggt tggttaaagc atctttaatt gatggaaaga
     4141 tcaatacgtt ctcattgtca gatgtaaaac ttgaacgtgt tacgaccgca tactttcggt
     4201 tgggtaatgc tcccattgat tcaaaagtct tgcgtccaac caacagccat tggttatagg
     4261 taatagcttt aaacaggagc tgttcacctt tggcactcca tggaatatca gggccattcc
     4321 cgataactcc attcttcgat atagctacca ttagtgatag tttcacaatt cttcctcaga
     4381 ggttaacatt ttattagtaa gcatgctcgt tttgtcaccc gctgatgctt accgttaatt
     4441 aatataatca cttgtattaa atagactttc tttaaaaata caagacactc tgttattaca
     4501 aatcgtgcat gccgtctatc ctgcttgcac gatgcacatt atctcaccca aagaactcat
     4561 aacattttaa tttatttagc attaaattaa aactctaccg gtaaacaagc atctctaggc
     4621 gtaaaaaaca ggcacttttt taaatcccag ctagaatagg ctgtataggc agacagttgc
     4681 aagacaagga gcgttttatg tctaacagtc catttttaaa ttctatacgc acggatatgc
     4741 gacaaaaagg ttatgcgctg aaaactgaaa aaacttacct gcactggatt aagcgtttta
     4801 ttctgtttca caaaaaacgt catcctcaga ccatgggcag tgaagaggtc aggctgtttt
     4861 tatccagctt agcaaacagc agacatgtag ccataaacac gcagaaaatc gctttaaatg
     4921 ccctagcttt tttgtacaac aggtttttac aacagccgtt gggcgatatt gattatatcc
     4981 ctgcaagcaa gcctagacgg ctaccctctg ttatctctgc aaatgaagtg caacgcattt
     5041 tgcaggttat ggatactcgc aaccaagtta tttttacgct gctgtatggt gcaggtttgc
     5101 gcattaatga atgcttgcgt ttgcgggtta aagattttga ttttgataat ggctgcatca
     5161 ctgtgcatga cggtaagggt gggaaaagca gaaacagcct actgcccacg cgcctaatcc
     5221 cagcaataaa ataactcatt gagcaagcgc ggcttattca gcaagacgac aacttacaag
     5281 gcgtagggcc atcgctgcct tttgctttag atcacaaata cccttctgct tatcgacaag
     5341 cggcgtggat gtttgtcttt ccctccagca cgctctgcaa ccacccgtat aacggcaaat
     5401 tatgccgcca tcatctgcat gactccgttg cgcgaaaggc attgaaggca gccgtacaaa
     5461 aagcaggcat cgttagcaag cgtgtcactt gtcatacatt tcgtcactcg tttgctacgc
     5521 atctattaca agcggggcgt gatattcgca ctgtgcaaga actcttaggg cataacgatg
     5581 ttaagaccac gcaaatctat acgcatgtgt tgggtcagca ttttgccggc accaccagtc
     5641 ctgcggatgg actgatgcta cttatcaatc agtaaaagta gataaatatc tttatgtagc
     5701 cctgagtagc ccctgatact ttttatggtg aaaaagataa acaatcatag aggggataaa
     5761 tatgagccag ttctaccatt gttcagataa gtttaagcaa gatactgtca atcacagtga
     5821 ccattcatgg tcattacact tcctccagtt gccagacaat gcctaacata aaaacgcaaa
     5881 agcccaaaag tcagagctta ggtgttctag gcgttttcta agcgctgaag aaatacgtct
     5941 taccgtagat tgcgtgcgtt tctgaagcac attttcctta tacatggctt tatcccaggc
     6001 agcttcatca agctcttgga gtaacaagcc agtaataatg cgacactctc gggtttgtag
     6061 cgagccgctg gtgagatcgg agttatagcg gaaaggggtc atttgtaatt atttgtgggt
     6121 ggaggatagt taacttatta ttgtagttgg aagaggctgc tttccaaaca ttagcacctc
     6181 gtaatgcttg ttactgtata ttaatttatg ctcttagcgc atgttaatgg gctattacaa
     6241 tgcataatgt ccacatcaag ataactgtgg cttaacacct gtacaagcga aaaaaatcaa
     6301 tcacctgtcc ggaaatacgg gatcactgta aagtgttggt gaggcagtct aggaggggga
     6361 tttaatttgg gatactgatg aaaggattat gatcagttgg atataggatc gtactaagct
     6421 gagcggcagg ttagctagat tagttaacca gataagtgaa atctagttcc aaactatttt
     6481 gtcattttta attttcgtat tagcttacga cgctacaccc agttcccatc tattttgtca
     6541 ctcttcccta aataatcctt aaaaactcca tttccacccc tcccagttcc caactatttt
     6601 gtccgcccac aagaacgcag catggtatcg gcatcaatca tggcatcgcc aaaccataat
     6661 accagcttta atgccatgct caaacggtgc ttggcgcttc ctcccgttgc cagatcgtgc
     6721 caaacacaaa caaaaaaaag cccgcaatta cgcgggctta gcggcatttc atgccatcag
     6781 gtgcctggct gtgccagacg cggaatcatt cctgccagac atgctgatta attttgccct
     6841 tattaacatc acagacagaa agaacgaggg taccaatacc attgatggta actggcaggc
     6901 cgatgaagga cgccgttacc gggataatgt aagaatctat ttttgagtat gtcaacataa
     6961 cctgaggcaa agtacctcag gttccagtgt agtaatcagc caacctgaca gtcttctgtt
     7021 agccctacag atatgtcgtg gagctaaccg cgcatgtcaa agccggattt ttgaaaatct
     7081 ctataaactt ccggattgtt gaaggccggg aatttagctt tatgggctgc tgactttatc
     7141 gcttcgcaat aggctttatc gccattactg gttgatattt ttaacgccgt gccatcccgg
     7201 gagaattcca tatgcaacct gcattttttc cttttccatt tttgcggatc atcaagttta
     7261 gcattaattg catccctgat tccccgtgct tgctcccccc attcatcctg atcatcccaa
     7321 cgtcctgaac tgcaactacc cagggctgta gttttatggc aatttgaagg atgtaatggc
     7381 gcacatcccg ccacaaagct gactaaaata gccagcataa cgattttctt tacccccata
     7441 tattgctcct caatccatat agatctcagt accatagctc tgtcagagca aagaaaatct
     7501 taactaatac aagttaagat ttttaatatc cactcctcat ccctacattc caggtgatgg
     7561 tggcgtccac tcattatcct gaactggagc ataagatgcg ccatgccccc cggatatgct
     7621 tttctgttgc ctgatatatt cgtggtagta ctgttcacta cgctgacgat gtaaatccac
     7681 atccgggcgg ctgattacaa agtgtccact tccggttatt ccagctgcat tatgccaggc
     7741 caccatttcc gcaaatacat cattaaactg ttgatatgag ccttccggtt caaaacccat
     7801 tcttggctca cgatcaaaca atacaggagc aatcagattc agcttatcca ccagttcatt
     7861 tctgtcctga tgatggtaga acaccacacg gtccccataa tccacacaga ctaactcgcc
     7921 attcctccga tcacgaaaac gcacactgcc atctttctgc agacgggctt cgagaacgcc
     7981 agtgtcttca taaagtggag ttcctcccgg ttcacagaga atcacgcagc ggtcggcatt
     8041 cgttgttcgt cgcttgtctt tgcgacgatc acgataatcc cagccacgta actgtgataa
     8101 tgcagctctg tctccctgta ccgcctgttg ctccacccac tgccggtagg acaaaggata
     8161 ccacttaccc tccgcaatca gtgacaaccg ctcttccttc acactctcct tcagcctgat
     8221 cagcgcctgc atacgctgca cttctgcaat atggtaatgc agcttacgca attgtggatc
     8281 ccggaactgc acacggatat acgccttgcg ccgacggcag gcagcatgaa tttcccgtaa
     8341 ccgttctccg taacgtaagt cgggtttacg ccagtgctcc ttccatgcaa gataacgcgc
     8401 gcgcaaatct tcccgcgcag ctgcacgggc ttcacggcgt tcacgacgca gtacaggatc
     8461 gcgccggaag ccgagctcca cttcatcgct ggcagcaagt tccggattgt agcgacattc
     8521 aggcttcacc cgttcaaata tatctgcacc tgcaatctca aatggccctg cctgcggctc
     8581 agccctggat aacgtcaaat ccgggtggat actgctggcc ttcactggtg tgagctcatg
     8641 attaaacgca tccacaatca caagaccatg atgctgtttc tgcaacataa gccctttacg
     8701 cgcaaacatc tcatggcacg cctgccagct gaaattcccg cccgcgatca tatcttcaat
     8761 caacgactca cactgaacag tcgtgtaccc ccacagactt tccttatcgg aaaacagttc
     8821 gcgctttatt gctccctgtg gacctgtccc catccgaata cgataccatt cgtaatcact
     8881 gacagcctga gtaccaagat tacgtccccg gaatcgttct ccgggtataa cctgagtaaa
     8941 aatatccttc ggtacaggct gccatccacc atccagatcc tgcatataat ccagcgtcat
     9001 taacggccac acactgtctg cacgtactgg tgtctgttgt ttatcaaatc catcatgcag
     9061 taccagatgc ccgtgctgga tccccatcca taatccagcc tttgccagag ttctgtgcac
     9121 atccaggcaa ctttccagct caccatttct cgccatttcc ggtaagcgtt cggcaaaatg
     9181 acggcaggcg tactgtttca gactctccgt ttctgccact ttttccggcc ggatatcaac
     9241 gtttatggct tccggatcat aacgaccggg tgtccccacc tgactgaaaa tatctgtcgg
     9301 tacaggctga tattccccca gtttccgccc cagtttttca gctgtccatg acggtccgaa
     9361 cgaactgagt gcaacacctt cccgcgctct gtcccagcca tccttcacca ccagttcccc
     9421 ttcctgcatc gtgatataca gaccatcact ggccagccgt ttatgcaaag acaaccagtc
     9481 ttgtgcgggc tcttcacgca gcccggcaat cgacatctgc gcaatatatt ccctgaacgt
     9541 ctgctttttc ccgcgtcgcc aggcattacg gcgctcacgt accagcgctg ttttgcgcac
     9601 aatacggttg ccgggtgcat gcacaaagca gccattatca ggcgcgaatc cgtgcttaag
     9661 ttcaagttca cgacatgcac ggctgagttt ttcctggctc caggaaagcc agttaatata
     9721 accagtttca ggatgtacac gattcacggc aacatgcaca tgcagattat ccgtgtccgt
     9781 atgaacggca gccacgtact gatgttttga gagttcaaga gatttcagtg tgtgacgcac
     9841 acagtcaaaa agctgctcag gacgcgggct ttcatgcgca ggccaggaaa gaatgtagtg
     9901 gaaaacgggg tctgtatcat tacgggaaaa aacagctttg tccgccttgt actgcatctc
     9961 ttcagcagct gtttccagtg aattgcagtt atggaaacag gttacgccat agaaatttac
    10021 ccactgactg ccgtccttca tcacatcaat cagggagaca aatttttcgt tacgcaggcg
    10081 tgttgcataa tcaacaagcc gtctgaaacg gttgcggtga gggacatcac tcttgatttc
    10141 gctgtcttct gtgagctcat tttcctgcac atcatcccgc acagacgtgt acgcaatcag
    10201 gtcttcaaag gaagacctgc cgtcacgtct tttgtgcgga ataatggcat tcatcagcag
    10261 ttatctcagc gtcccattaa gtctcttcgc agagcattga catattcagt aatggcaaca
    10321 agaacctctg catactcctt atcaccagtg cgttttccct ccacaaacag atgcttctga
    10381 aggcggccaa gacgcataat ctcatcaatc atacggtcgt cagtacggca gttaaccacc
    10441 ttatccagtg aagaacttcg tataaatgtt gaaaccgtct gtccacttga ctccgctttt
    10501 tctcttacga tttcagcctc atcctccgtg aatctcgccg tgaattttac aattctcttc
    10561 ctgttctcac tgcccgttcg ggttttcttc tcactcatgg tctgctcctt accggggtgt
    10621 cggggcaaag ccctgaccag gtggggaatg tctgagtgcg cgtgcgcggt ccgacattcc
    10681 cacatcctgt cccgtttttc aggccattat agcctgttat ttttgtgcat catagtctta
    10741 tgcagacggc agaggcaggg cgcagccctg ccaggagtca gtttttggtg ataaaatcag
    10801 gcttcaatat taaacggaga gaaagtgcca atggcgtcta aaaaatttta ctcagatgat
    10861 gacatccagc tggcaaaagc tgctttatcg gaattgccag atctcaccgc tcaacggaaa
    10921 acattgcgtg attttcttga tgccattcgt gatgacatta ttgtcctggt aaggacaaag
    10981 ggttatacac tggcagatgt tcgtaacacg ctcaaaaatg cagggtatga agtaggagag
    11041 aaagcacttc gtgacattat tcgggaagca gaatcgaaaa aaccatcccg ccgttcttcc
    11101 tctaaaacag cacctaaaaa gacagattca ggcaggaaag atggcatcga tatgaacaac
    11161 aattgatgtg acaatgtcac atcgtgttct gagattatga tgattacaga aaaaaaagcc
    11221 ccggcataaa taccagggct tttctgttta atcagactcg tggcggcaat acacctggat
    11281 aatgccgtgc aataatatca ttcagttttt caacaaggtc agaacgctta aagacaatat
    11341 gtacggtccc tttctggtag ccacgcaccg taaaatattc cccttctaac agcttaccga
    11401 cattatcgcc gttcccgaga aattcaccat actgcgcccc gatgctgttc ctgaagtcag
    11461 gaacgttgcg cccctccagc agataaaacg gacgtaccag gtcatccagt gcctcccgtc
    11521 cgctgcagtc cagcgtcaca cgcccgttac tccagcggta cagcaggttt tcaataatga
    11581 ttttcttgcc cagacggcag ggattattgg ttctgtaatc ccatgacaat ttccggtaga
    11641 catcaatcag tccctgttca aacgtctgca tcttgctggc attcagatgg cggaatgttg
    11701 ccagtacatt gtccagggtg atttcaggac aggtgtcgct catcagctgg ctgttccact
    11761 catcacgctg gcatgaactc atgaaggtgt acatgcccgt atctgtcatc aggcggtccc
    11821 agattttccg gtcggcaaga cggatgatat tccttttcgc ttcttccggt ttatccatgc
    11881 atcgaacgga gtcagtcaca cagtcttcaa acccgtagag aaaaatgtca ccggaagcat
    11941 cgagcagaat cctgcgcgct gtcgccaggc attccatcgc ctgcatgaac agtgcaatac
    12001 cttcgttacg ctgcgcgatg atgcggtcga tagccacaga cggaataaca tctgtcgtgc
    12061 cggcagtatt caggtcagca gtgggtaaag cctgattcat cacattgtct cctcatcgtc
    12121 acgactgccg ggtcagccag gtcatttccc ggaggagcga agctccttct gccgtccggg
    12181 tcttagggta tttccccgca gtggaccttt ccgacggggg acgctcacca cgtcacgggg
    12241 cagttgtccg ggggcagatg caaaagtttt tgacgccagc ggcaaaaagt tttgtggctg
    12301 gtacatctct cccctggacg actgaaacgg gatgtggtcg tgtatgtacc cccggaaagt
    12361 ccgtctgcgg agaacaggcc gcagtgcggc ggcagacagg cagagacagc tgcgccatca
    12421 ggcgcagtgg ccttacacac cgtcaggtgt gcagggagcg ccgcatacgg cgcgactgaa
    12481 gaaaaacgac ggcacaacaa tggcatgtgg tggtcactga tgcaccactc ccgcacaggt
    12541 gaaaacagag aaaacagcac gacatttgca actagaaaag tgaaatttct tcactttttc
    12601 actcttattt ttcgcgctga attggtgatt ataatgataa aataaaatga agttttttca
    12661 ctttttaaaa ggtactttat gaccggctgg gaactgcgaa tctggcgaaa aagcatgctc
    12721 tggtcacgcg aaaaagcgtc cagagaattt ggcgtaacac agagaacatg gcacgcctgg
    12781 gaaaatgcgg aacaggtgga cgtcaccgtc tggaggacca cccaggcact ttcagtccgt
    12841 gacctgctgc ctcacatgca ggggatgcgc aaggcagata tcatcaggcg tctggaaaat
    12901 gaactggggg aaacggcaga ggatgtgtga gcgtgattca cagcctggct gaatgccagg
    12961 ctactgctca tcaggaaaca gctttttcag ttcttcaccg gaaagtcccg tgacctcctg
    13021 aatccgttcc ggcggccagc ctatatcccg cagccggcag gctatttccc gcgccacttc
    13081 aagaacccct tgtttaaaac cttgtttaaa accttcttta aaaccctcgt cgaaaccttt
    13141 ctgtataaga cgttctgcaa tcgtcatgct gtttgctctg ctcctgctga caaaaaagcc
    13201 cgggattttg ccgggctttg tggttctcat cagtgagtga ttttcttcag ttcgtcatcg
    13261 gaaagtccgg tagcctgctt aatctgttcc ggcgtcatcc ccattttctg aagctggcac
    13321 gcaatagcca gggctttttc aagcgctcct tccatgcgtc cttccatgcg tccttccatg
    13381 cgtccttcca tcctgccttc ctggcgacct ttttgctcaa gacgttctgc gatagtcatc
    13441 agtttatcct catgctgcgg cagacgctca gccagcgccc gcataaattc cggtgataca
    13501 gagtcccctg cgttaacgat gtaatgaatc agaacacgca cctgctcgtc tgaaatttcc
    13561 accatgacct gcgggagttt gtccagcagc tccatcatgt cacgatggcg gatatgcttc
    13621 atcagcagcg tcagtgcggc catccggcga tggttcatga tttcattgtc atcaacgaca
    13681 gtgatatcaa ccagcggaaa cggctttgtg tatattttag ccgcaagtgc cggattctca
    13741 aaacagtcca gccagttcat gctgtacggg tacgggctgc gctcaccgtg atagaacagc
    13801 accggaatca ccagtggcaa cgttttgtgc tgctccagat gtctctgcat cgccgccaca
    13861 ctgtagcgta acatgcgaaa aggcatgtac agattttcgg tgctctggtg ctcgatgagc
    13921 gtataaacat agccgtcttc acccccggta gttttcacgc tccagaggat atcgctggcg
    13981 tactgatgca ggtccggctc tacaaaggtg gcgggttcca gcttcagcgt ggacaggtcg
    14041 cacaactgcc ggtactccgc cggaagatgc agttccagaa aatctctggc gacgtcggga
    14101 ttcgccagga acgaccggaa cgcggcatca tgcggcgtag gcgtcgtggt gtttttcttc
    14161 ttactcacgg ttcctccatc aaatcagcag catcgcagca atcatcgccc aaatccccac
    14221 aaccgagagg acagcaattg cgttcaaagc catgacaaca atagccgcgt tctgagtcac
    14281 ggcgtatttc ttcagcaatc tcttctgcgg aaaggtaatc aaactcctca cgggccagtt
    14341 ccaggaccag tagatgttgt tctgtcggca atgtggccac aaaattatca atagcagcac
    14401 tcaaacgtcg ctgatatagc tcataaacaa gaccacaacg gggcattagt tcttcggata
    14461 atagttccag ttttgagcga attctgtcag cgttgaaagt gacttcatca aaagacatat
    14521 gaaaagcttt gtcagtatca aaacaagact tcattgtgaa ccgccccggg gatcctggag
    14581 actaaactcc ctgagaaaga ggtaaacagg atgactaaaa atactcgttt ttcccccgaa
    14641 gtccgtca
//
LOCUS       DBKEKI010000093        14607 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.093, whole genome shotgun sequence.
ACCESSION   DBKEKI010000093 DBKEKI010000000
VERSION     DBKEKI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.093"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(177..1370)
                     /locus_tag="ACUQPX_002860"
     CDS             complement(177..1370)
                     /locus_tag="ACUQPX_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9057515.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1506..3230
                     /gene="iucA"
                     /locus_tag="ACUQPX_002861"
     CDS             1506..3230
                     /gene="iucA"
                     /locus_tag="ACUQPX_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HHV9057516.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3231..4178
                     /gene="iucB"
                     /locus_tag="ACUQPX_002862"
     CDS             3231..4178
                     /gene="iucB"
                     /locus_tag="ACUQPX_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HHV9057517.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4178..5920
                     /gene="iucC"
                     /locus_tag="ACUQPX_002863"
     CDS             4178..5920
                     /gene="iucC"
                     /locus_tag="ACUQPX_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HHV9057518.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            5917..7254
                     /gene="iucD"
                     /locus_tag="ACUQPX_002864"
     CDS             5917..7254
                     /gene="iucD"
                     /locus_tag="ACUQPX_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HHV9057519.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7260..9455
                     /gene="iutA"
                     /locus_tag="ACUQPX_002865"
     CDS             7260..9455
                     /gene="iutA"
                     /locus_tag="ACUQPX_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HHV9057520.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10022..10165)
                     /locus_tag="ACUQPX_002866"
     CDS             complement(10022..10165)
                     /locus_tag="ACUQPX_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057521.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10415..14302
                     /gene="sat"
                     /locus_tag="ACUQPX_002867"
     CDS             10415..14302
                     /gene="sat"
                     /locus_tag="ACUQPX_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HHV9057522.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTANHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNASNANFRYSF"
     gene            14349..>14607
                     /locus_tag="ACUQPX_002868"
     CDS             14349..>14607
                     /locus_tag="ACUQPX_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057523.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILDHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGG"
BASE COUNT         3896 a         3499 c         3642 g         3570 t
ORIGIN      
        1 gggatcatag gttgttgcac gaaagaatca tggagattac accgcagcaa ctggttgaat
       61 tcactatacc tgatgggcgg gggcagcaag ccagaacact gcctgacatg ctttcatcag
      121 acgcaggtcc tctctcatat cctctgattt actccagtaa ggtgatgatc atatctttat
      181 ttgatagtac ggtaatgcca gataaataca gacacaatag taaataatat agttgcaaat
      241 aatgctgcca taatcagcgc gattgcgaca cctgttttcg ataccagcgg caccagcaaa
      301 gcgcacagtc catagccaag tgtgtgactg gtagcgagcc agcccgcgcc tgcgccatca
      361 gccagcttat cattcagtag aagttgataa gcgggtgttg ccagagctgc tccaaatgac
      421 agcactgcac agcccgggta aaacaaccat agctgttcgg agagcatgat agccagacca
      481 ccactcatca acactccggc actcaggagc agggccaccg gagtcaggcg ctgcggacgg
      541 agtaccccga actgcgcgat aagcgcagct actgcggaca gacccaacag ccaggccacc
      601 tgttggctaa tggcggtggt atccgtagcg aactggcgag taagggcagg cgaaagtcca
      661 agctgcatca tgctcaccgc cgcagccagt aatattgcgc aaagcagata aggcagacaa
      721 tcccgcttca gactgacgct cttgcactcc ggtgtgggct gtggtggtgt gccgggcaac
      781 cgcagcagca tcagcagcgc cagcactggg gctgccatca gcagccccag tggcgccaga
      841 ggatgaatgg ccagcattgc tgccgcgcac agcggcccga atagcctgcc gcaactcagg
      901 ccggagctga tggttgccag agcggccatg cgattccctt ctccagctct ctgcaatgcc
      961 cagacctgac aggctggcac cattgccgac acggtcagcc cgtaggcgat ccgggcgatg
     1021 attaatattc ccagtcccac cgctgttgtt atggcgctgg tagccatcag cacgcttccc
     1081 agccctagca gggtaaagct tatgccatat cctcccagag cccacaacac cacaggttta
     1141 ctgccagtac gggaaatttg ctttccccac cacggggacg atggcagaaa cagcattgag
     1201 ccaagcatca gcagggcagc ccatacagac agactcagat ttgtctgtat gaccagaaca
     1261 gggagcacaa ccagcagacc gttctgtccg ataccgagaa gcccggcact gaacgcaagt
     1321 ggccagcagg acagtggttt ttggggcgta tcttcgatcc ctggtgacat tataatgttt
     1381 caactccatg tattaattat gtttatttgt aaaattaatt tatctgacaa taacattcat
     1441 tgttgataat gagaatcatt attgacataa ttgttattat tttactgtgt gggagctgtt
     1501 tgactatgac cctgccctct gaaaaaccag ccacagatgt ggctgcgcag tgcttcctga
     1561 atgcactgat tcgtgaaacc acagactgga aactgacaga atacccgcca gacgaattgc
     1621 tcatcccgct ggatgagcag aaatcgctcc atttcagagt ggcttatttc tccccaaccc
     1681 agcatcaccg ctttgcattt cctgcacgtc tggtcacggc atcaggcagt tatcctgtcg
     1741 actttaccac tctctcccgg cttattattg ataagctacg ccatcaactg tttctgcccg
     1801 ttcccctctg cgaaactttc caccagcgcg tgctggaaag ccatgtccat acgcaacagg
     1861 caattgatgc ccgtcatgac tgggccgctc tgcgtgaaaa agcgttgaat tttggcgagg
     1921 ctgagcaggc gctgctgaca ggacacgctt tccaccctgc gcctaagtct catgaaccgt
     1981 ttaaccggcg ggaggctgaa cgctacctgc ctgatatggc accccacttc ccactgcggt
     2041 ggttttcggt ggataaaacg caaatcgccg gtgaaagttt gcatctcaac cttcaacagc
     2101 ggctgacgcg atttgccgca gagaatgcgc ctcagttact caacgaatta agtgacaacc
     2161 aatggctgtt cccgttgcac ccgtggcagg gggaatatct tttgcagcag gggtggtgcc
     2221 aggcacttgt tgctaaaggg ctgattaaag acttaggtga ggccggcacg tcgtggctgc
     2281 cgaccacctc ttcccgttcc ctctactgtg ccaccagccg cgatatgatc aagttctccc
     2341 tgagcgtacg gctgaccaac tccatccgta ctctgtccgt gaaagaagtg aagcgtggga
     2401 tgcgcctggc acgcctggct caaaccgacg gctggcagat gctacaggtc cgcttcccga
     2461 ctttccgggt aatgcaggag gatggctggg ccgggctgct cgatcttaac ggcaacatca
     2521 tgcaggaaag tctgtttgcc ctgcgtgaaa atctgctggt ggatcagccg aaaagccaga
     2581 ctaacgtact ggtctccctg actcaggccg cacctgatgg cggtgattcg ctgctggttt
     2641 cggcggtaaa acgcctgagc gatcgcctcg gtatcactgt gcaacaggcc gcccatgcat
     2701 gggtcgatgc atactgtcag caagtgctaa agccgctgtt tacggctgaa gcggattacg
     2761 gcctggtgct gctggcgcat cagcaaaata ttcttgtcca gatgcttggg gatctgccgg
     2821 tcggatttat ttaccgtgac tgtcagggca gcgcttttat gcctcacgcg acagactggc
     2881 tcgattctat tggcgaggcg caggcggaaa atattttcac ccatgagcag ttgctgcgct
     2941 atttccctta ttacctgctg gttaactcca cttttgctgt gaccgctgcg ctgggggctg
     3001 ccgggctgga cagcgaatcg aatctgatgg ctcgtgtacg agcatcgctg gctgaagtgc
     3061 gtgatcaggt gactcataaa acctgtctca actacgtgct ggaaagtccg tactggaacg
     3121 taaaaggtaa ctttttctgt tatctgaacg atcataacga gaataccatt gttgaccctt
     3181 cggtgatcta tttcgatttc gctaacccgc tgcaggctca ggaggtctga atgtctgagg
     3241 caaacattat tcacagcaga tatggactgc gctgtgaaaa actcgacaag cccctgaatc
     3301 ttggctgggg actggacaat agcgcggtgt tgcactgtcc cggggagctg ccgacagggt
     3361 ggctgtgcga tgcgctggat cagatattta tcgccgcacc acaactgtca gcagtggctc
     3421 tcccctgggc cgaatggcgt gaggagccac aggcgctgac gctttttggg caggtaaaaa
     3481 gcgacattat ccatcgtact gccttctggc agttaccgtt atggttgagt tcaccggcaa
     3541 accgggcctc cggtgaaatg gtttttgatg cagagcgtga gatttatttc ccgcagcgcc
     3601 caccccgtcc acagggtgag gtttaccgcc gttatgatcc acgtattcgc aggatgctga
     3661 gtttccgcat tgccgatccc gtttctgatg cagaacgttt cacccgctgg atgaatgatc
     3721 cgcgcgttga gtatttctgg gagcaaagtg gctcactgga ggtacagacc gcctatctgg
     3781 agcgccagct caccggtaaa catgcattcc cgctgattgg gtgcttcgac gatcggccat
     3841 tcagctattt cgaaatctac tgggcggcgg aagaccgcat tggtcgtcac tattcgtggc
     3901 agccctttga ccgtggcctg catctgctgg ttggtgaaca gcaatggcgc ggggcccact
     3961 atgtgcaaag ctggctgcgc gggctgacac attacctgct gctggatgag ccccgtacgc
     4021 agcgcacagt actggagcca cgcaccgata accagcgcct gttccgccat cttgagcctg
     4081 cgggataccg gacaattaaa gagttcgact tcccacataa gcgctcgcgc atggtgatgg
     4141 cggatcgtca tcacttcttc acggaggtcg gtctgtaatg aatcacaagg actgggattt
     4201 tgttaaccgt cggctggtgg caaaaatgtt gtctgagatg gagtatgagc aggttttcca
     4261 cgcggaatct cagggcgatg accactactg cattaacctg ccgggagcac aatggcgctt
     4321 catcgctgaa cgtggtatct ggggctggct gtggattgat gcccaaacgt tgcgctgcac
     4381 ggacgaacca gtactggcgc agacgctgct gatgcagcta aagccggtgc tgtcaatgag
     4441 tgatgccact gttgcagagc atatgcagga tttgtatgcc acgctactgg gcgacctgca
     4501 actgctgaag gcccgtcgcg gactgagtgc cagtgacctg attaatcttg atgccgatcg
     4561 tctgcaatgc ctgctgagcg gtcatcctaa attcgttttt aataaaggtc gccgtggctg
     4621 gggcaaagag gcgctggaac gatatgcgcc agagtatacc aacaccttca gactgcactg
     4681 gctggcggta aaacgtgaac atatgatctg gcgctgcgat aacgatctgg atattcagca
     4741 gttgttgact gccgcaatgg atccgcagga gtttacacgc ttcagtcagg tctggcagga
     4801 aaacgggctg gatcacaact ggctgccgct gccggtacat ccgtggcagt ggcagcaaaa
     4861 aatcgccaca gacttcatcg ctgattttgc cgaaggcagg atggtgtctc tcggcgagtt
     4921 tggcgaccag tggctcgccc agcagtcgct gcgtaccctg accaacgcca gccgccgggg
     4981 agggctggat atcaagctgc cgctgaccat ctacaatacc tcatgctacc gggggatccc
     5041 tggcagatat atcgctgccg gaccactggc ttcacgctgg ctgcaacagg tttttgcgac
     5101 cgacgccacc ctggtgcaaa gcggcgcagt gatccttggt gaaccggctg caggctatgt
     5161 gtcccatgaa ggctatgccg cgcttgcccg ggctccctat cgctaccagg aaatgcttgg
     5221 tgttatctgg cgggagaatc cgtgccgctg gctgaaaccg gatgaaagtc ctgttctgat
     5281 ggcaacactg atggagtgcg acgaaaacaa tcagccgctg gcaggcgcat atatcgaccg
     5341 ctccggactg gacgctgaaa cctggcttac gcaattgttc cgggtggttg tggttccgct
     5401 gtatcacctg ctttgccgct acggtgtcgc gcttattgca cacggacaaa atataacgct
     5461 cgccatgaaa gagggggtac cacagcgtgt tctgctgaaa gacttccagg gcgatatgcg
     5521 gctggtgaaa gaagcgtttc ccgaaatgga ctctttgcct caggaggtgc gtgatgtcac
     5581 atcccgtctg agtgcggact acttaatcca tgatttgcag acgggtcact tcgtgacagt
     5641 actgcgtttt atttcgccac tgatggttcg tcttggcgta cctgaaaggc gattttatca
     5701 actgctggca gcagtgttga gtgattacat gaacaaacat ccacaaatgg cagagcgttt
     5761 tgcgcttttc tcactcttca ggccacaaat cattcgcgtg gtgctgaacc cggtaaaact
     5821 gacctggccg gacctggatg gcggcagtcg catgctgccg aattaccttg agaatctgca
     5881 aaatccgctg tggctggtaa ctcaggaata tgaatcatga aaaaaagtgt cgattttatt
     5941 ggtgtcggga cagggccatt taatctcagc attgctgcat tgtcacatca gatcgaagaa
     6001 ctgaactgtc tcttctttga cgaacatcct catttttcct ggcatccggg tatgctggta
     6061 ccggattgtc atatgcagac cgtctttctg aaagatctgg tcagtgcagt tgcacctaca
     6121 aatccctaca gctttgttaa ctatctggtg aagcacaaaa agttctatcg cttccttaca
     6181 agcagactac gtacagtatc ccgtgaagag ttttctgact atctccgctg ggctgctgaa
     6241 gatatgaata acctgtattt cagtcatacc gttgaaaata ttgatttcga caaaaaaagt
     6301 cgattgtttc tggttcagac cagtcgggga gaatattttg cccacaatat ctgccttggt
     6361 acaggaaaac aaccttattt accaccctgt gtgaagcatg tgacacaatc ctgtttccat
     6421 gccagtgaaa tgaatcttcg tcggccggac ctgagtggaa aacggataac cgtggttggt
     6481 ggaggacaga gtggtgcaga cctgttcctt aatgcattac gcggggaatg gggagaagcg
     6541 gcggaaataa actgggtctc acggcgtaat aattttaacg cactggatga ggctgctttt
     6601 gctgatgagt attttacacc tgaatatatt tcaggcttct ccggactgaa ggaagatatt
     6661 cgccatcagt tactggatga gcagaaaatg acatcggatg gcatcactgc cgattcttta
     6721 ctgaccattt atcgtgagtt gtaccaccgt tttgaagttc tgagaaaacc aagaaatatc
     6781 cgtctgctac ccagtcgctc ggtaacaact ctggaaagta gtggtccggg ctggaggtta
     6841 ttgatggaac atcatctgga tcagggcaga gagagcctgg aaagtgatgt ggtgattttc
     6901 gccacaggtt accgttctgc attgccacaa atacttccct cactgatgcc cttgatcacc
     6961 atgcacgata agaacacctt taaagtgcgt gatgacttca ctctggaatg gagtggcccg
     7021 aaagaaaata acatctttgc ggtcaacgcc agcatgcaaa ctcatggcat cgccgaaccc
     7081 cagctcagcc tgatggcctg gcgatctgca cgtattctta atcgcgtact ggggcgtgat
     7141 ttattcgatc tcagtatgcc gcccgcactg attcagtggc gcagcggcag ccggaaaaaa
     7201 ccgcagccgg aggctgcttc tttaactcac tatacagcaa atattcagga ataacgatga
     7261 tgcgcaaaaa gtatatgccc cgggctcttg gtccgctgct tcttgtcgtg ctgtcaccag
     7321 ctgtcgccca gcaaaacgat gataatgaga tcatagtgtc tgccagccgc agcaatcgaa
     7381 ctgtagcgga gatggcgcaa accacctggg ttatcgaaaa tgccgaactg gagcagcaga
     7441 ttcagggcgg taaagagctg aaagacgcac tggctcagtt aatccccggc cttgatgtca
     7501 gcagccagag tcgaaccaac tacggtatga acatgcgtgg ccgcccgctg gttgtcctga
     7561 ttgacggtgt gcgcctcaac tcttcacgtt ccgacagccg acaactggac tctgtcgatc
     7621 cttttaatat cgaccatatt gaagtgatct ccggcgcgac ggccctgtac ggtggcggga
     7681 gtaccggagg gttgatcaac atcgtgacca aaaaaggtca gccggaaacc atgatggagt
     7741 ttgaggctgg cacaaaaagt ggctttaaca gcagtaaaga tcacgatgag cgcattgccg
     7801 gtgctgtctc cggcggaaat gaccatatct ccggacgtct ttccgtggca tatcagaaat
     7861 ttggcggctg gtttgacggt aacggcgatg ccaccctgct tgataacacc cagaccggcc
     7921 tgcagcactc caatcggctg gacatcatgg gaaccggtac gctgaacatc gatgaatccc
     7981 ggcagcttca actgataacg cagtactata aaagtcaggg agacgacaat tacgggctta
     8041 atctcgggaa aggcttttcc gccatcagcg ggagcagcac accatacgtc agtaaggggc
     8101 tgaattctga ccgcattccc ggcactgagc ggcatttgat cagcctgcag tactctgaca
     8161 gtgatttcct gggacaggaa ctggtcggtc aggtttacta ccgcgatgag tcgttgcggt
     8221 actacccgtt cccgacggta aatgcgaata aacaggcgac ggctttctcc tcgtcacagc
     8281 aggataccga ccagtacggc atgaaactga ctctgaacag ccaactgatg gacggctggc
     8341 aaatcacctg ggggctggat gctgagcatg agcgctttac ctccaaccag atgttcttcg
     8401 atctggctca ggcaagtgct tccggagggc tgaacaacca taagatttac accaccgggc
     8461 gctatccgtc atatgacatc accaatctgg cggccttcct gcaatccagc tatgacatta
     8521 atgatatttt taccgttagc ggtggcgtac gctatcagta tactgagaac agggtagatg
     8581 atttcatcga ctacacgcag caacagaaga ttgctgccgg gaaggcgata tctgccgacg
     8641 ccattcctgg tggttcggta gattacgata actttctgtt caatgctggt ctgctgatgc
     8701 acatcaccga acgtcagcag gcatggttca atttttccca gggggtggca ttgccggatc
     8761 cggggaaata ttatggtcgc ggcatctatg gtgcagcagt gaacggccat cttcccctga
     8821 caaagagcgt gaacgtcagc gacagtaagc tggaaggcgt gaaagtcgat tcttatgaac
     8881 tgggctggcg ctttatcggt gacaacctgc ggactcaaat cgcggcatat tactcgcttt
     8941 ccaataagag cgtggaaagg aataaagatc tgaccatcag tgtgaaggac gacaggcgcc
     9001 gtatttacgg cgtggaaggt gcggtggact acttgatccc ggatactgac tggagtaccg
     9061 gtgtgaactt caatgtgctg aaaaccgagt cgaaagtgaa cggtcaatgg caaaaatatg
     9121 acgtgaagga atcaagtcca tcgaaagcga cagcttacat taactgggcg ccggaaccgt
     9181 ggagtctgcg tgtacagagc accacttctt tcgacgtaag cgatgcagag ggtaacgata
     9241 ttaatggtta cactaccgtc gattttatca gtagttggca gcttccggtg ggaacactca
     9301 gcttcagcgt tgagaacctc ttcgaccgtg actataccac tgtctgggga cagcgtgcac
     9361 ctctgtacta cagcccgggt tacggccctg cttcactgta cgactacaaa ggccggggcc
     9421 gaacctttgg tctgaactac tcagtgctgt tctgacgggg atgcttttgc aacaagggta
     9481 tgctgtaaac aacatggtcc ttggtggcca tgttgtttac agccagtatt gtttacagcc
     9541 agtataatat acagccttaa caagatattc cgtctccgga atggactgtt caccactgaa
     9601 taacattatg tctgttttat ggatctttga ctgaaattta tcgcatatct tcatttcatt
     9661 gtccgtctga aaactaacct tacaagtaag cgtcgtctca gcaccgtctg gcagatcctg
     9721 aaattcctga gaggatgatc tgcgcccaat atcctgagcc ggaactttgc tgcaatgaag
     9781 gccaacgaaa aatgggggag tatgttatag agtacttgct acagggtaaa aagaggtacc
     9841 tgtcgctggt tctccgttca gtttaaccgg gagattttct cctgcgcctg tcataaagtc
     9901 cggtaatgga gattgttaat acgatggtgc acgatgcatt gtcagtgccc ggcccggagg
     9961 atgccccgta gctacatacg gatcagggtt ggcaatatcg aatggcaggc tataggcaaa
    10021 gttaaagccc catggagtag cacaaaatat gccgcacaaa ggaaacggtc tgaataacgc
    10081 agtgatggag aacttcttcg gcacactgca aaatcgcatc gtggtcgatc gctaattcgt
    10141 cgtgaatcac tgaacgatgt tccatgcgaa cagatattcg atcatatcga aatattaaac
    10201 attacccctc cccacattgg gcaaaaaaaa acaaaaaacg atcaattaac gatcaattag
    10261 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10321 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10381 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10441 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    10501 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    10561 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    10621 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    10681 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    10741 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    10801 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    10861 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    10921 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    10981 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11041 atccaaaatc tcaacaaatt cagcatatag ccatgatctg ttaagtgcta gtctatttga
    11101 ggtcacccaa tgggactcat acggcatgat gatttataaa aatgataaaa catttcgtaa
    11161 tcttgaaata ttcggagaca gcggctctgg agcatactta tatgataaca aactagaaaa
    11221 atgggtatta gtcggaacaa cccatggtat tgccagcgtt aatggtgacc aactgacatg
    11281 gataacaaaa tacaatgata aactggttag tgagttaaaa gatacctata gtcataaaat
    11341 aaatctgaat ggcaataatg taaccattaa aaacacagat ataacattac accaaaacaa
    11401 tgcagatacc actggtactc aagaaaaaat aactaaagac aaagatattg tgttcacaaa
    11461 tgggggaaat gttctgttta aggataattt ggattttggt agcggtggta ttatctttga
    11521 cgaaggccat gaatataaca taaacggtca gggatttaca tttaaaggag caggaattga
    11581 tatcggaaaa gaaagcattg taaactggaa tgcattgtat tccagtgatg atgttttaca
    11641 caaaataggc cctggtactc tgaatgttca aaaaaaacag ggggcaaata taaagatagg
    11701 tgaaggaaat gttattctta atgaagaagg aacatttaac aatatatacc ttgcaagcgg
    11761 aaatggtaag gtaatactaa ataaagataa ttcccttggc aatgatcaat atgcggggat
    11821 attttttact aaacgtggtg gcacgctaga tttaaatgga cacaatcaga cttttactag
    11881 aattgccgcc actgacgatg gaacaacaat aactaactca gatacaacga aagaagccgt
    11941 tctggcaatc aataacgaag actcctacat atatcatggg aacataaatg gcaatataaa
    12001 actaacgcac aatattaatt ctcaggataa gaaaactaat gcaaaattaa ttctggatgg
    12061 tagtgtcaac acaaaaaatg atgttgaagt cagtaatgcc agtcttacca tgcaaggcca
    12121 tgcaacagag catgcaatat tcagaagcac agcgaatcat tgctccctgg tatttctttg
    12181 tggaacggac tgggtcaccg ttttgaaaga aacagagagt tcatataata aaaagttcaa
    12241 ttctgattac aaaagtaata atcagcagac ctcatttgat cagcctgact ggaaaaccgg
    12301 ggtgtttaaa tttgatacat tacacctgaa caatgctgac ttttcaatat cacgcaatgc
    12361 caatgttgaa ggaaatatat cagcaaataa atcagctatc acaatcggcg ataaaaatgc
    12421 ttacattgat aatcttgcag ggaaaaatat tactaataat ggttttgact tcaaacaaac
    12481 tatcagtact aatctatcca taggagaaac taaatttaca ggtggcatca ctgcacataa
    12541 cagccaaata gccataggtg atcaagctgt agttacactt aatggtgcaa cctttctgaa
    12601 taatactcct ataagtatag ataaaggagc aaaagttata gcacaaaatt ccatgttcac
    12661 aacaaaaggt attgatatct ccggtgaact gactatgatg ggaatccctg aacagaatag
    12721 taaaactgta acgccgggtc tccactacgc tgctgatgga ttcaggctga gtggtggaaa
    12781 tgcaaatttc attgccagaa atatggcatc tgtcaccgga aatatttatg ctgatgatgc
    12841 agcaaccatt actctgggac agcctgaaac tgaaacaccg actatatcgt ctgcttatca
    12901 ggcatgggca gagactcttt tgtatggctt tgataccgcc tatcgaggcg caataacagc
    12961 ccccaaagct acagttagca tgaataatgc gatctggcat ctaaatagcc agtcatcaat
    13021 taatcgtcta gaaacaaaag acagtatggt gcgttttact ggtgacaatg ggaagtttac
    13081 aacccttaca gtggacaacc ttactataga tgacagtgca tttgtgctgc gtgcaaatct
    13141 ggcccaagca gatcagcttg ttgtcaataa atcgttgtct ggtaaaaaca accttctgtt
    13201 agtcgacttc attgagaaaa atggaaacag caacggactg aatatcgatc tggtcagcgc
    13261 accaaaagga actgcagtag atgtctttaa agctacgact cggagtattg gcttcagtga
    13321 tgtaacaccg gttatcgagc aaaagaacga tacagacaaa gcaacatgga ctctgatcgg
    13381 ctataaatct gtggccaacg ccgatgcggc taaaaaggca acattactga tgtcaggcgg
    13441 ctataaagcc ttccttgctg aggtcaacaa ccttaacaaa cgtatgggtg atctgcgtga
    13501 cattaacggt gagtccggtg catgggcccg aatcatgagc ggaaccgggt ctgccggcgg
    13561 tggattcagt gacaactaca cccacgttca ggtcggtgcg gataacaaac atgaactcga
    13621 tggccttgac ctcttcaccg gggtgaccat gacctatacc gacagccatg caggcagtga
    13681 tgccttcagt ggtgaaacga agtctgtggg tgccggtctc tatgcctctg ccatgtttga
    13741 gtccggagca tatatcgacc tcatcggtaa gtacgttcac catgacaacg agtataccgc
    13801 aactttcgcc ggccttggca ccagagacta cagctcccac tcctggtatg ccggtgcgga
    13861 agtcggttac cgttaccatg taactgactc tgcatggatt gagccgcagg cggaacttgt
    13921 ttacggtgct gtatccggga aacagttctc ctggaaggac cagggaatga acctcaccat
    13981 gaaggataag gactttaatc cgctgattgg gcgtaccggt gttgatgtgg gtaaatcctt
    14041 ctccggtaag gactggaaag tcacagcccg cgccggcctt ggctaccagt ttgacctgtt
    14101 tgccaacggt gaaaccgtac tgcgtgatgc gtccggtgag aaacgtatca aaggtgaaaa
    14161 agacggtcgt atgctcatga atgttggtct caatgccgaa attcgcgata atcttcgctt
    14221 cggtcttgag tttgagaaat cggcatttgg taaatacaac gtggataacg cgagcaacgc
    14281 caacttccgt tactctttct gataacagcc cgggccgcgt ttgcggccct tcttctaccg
    14341 gagagaatat gtattaccct gtgacagact atatcgctct tgctctcatt attagctttc
    14401 tttttctgac attatttatc tgcctgttat gccttaaaca tgagcgaata aaaaaagaaa
    14461 ctatcaggca aaaaaatgca catattctgg atcatggctg gaatgcaact gagttctcat
    14521 ggttccgata cggacagtat aacgaaacgg gtatttacat ctcgatcgag aagaccatta
    14581 tcattacaat caccgtttct ggtgggt
//
LOCUS       DBKEKI010000094        14181 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.094, whole genome shotgun sequence.
ACCESSION   DBKEKI010000094 DBKEKI010000000
VERSION     DBKEKI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.094"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..622
                     /locus_tag="ACUQPX_002869"
     CDS             <1..622
                     /locus_tag="ACUQPX_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="catalyzes the transfer of
                     4-deoxy-4-formamido-L-arabinose from UDP to undecaprenyl
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HHV9057524.1"
                     /translation="ADEGYDVVVTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            622..2625
                     /gene="arnA"
                     /locus_tag="ACUQPX_002870"
     CDS             622..2625
                     /gene="arnA"
                     /locus_tag="ACUQPX_002870"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HHV9057525.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFADFRVVESSSYYGKSSSYYGKGYQDVE
                     HRKPSIRNAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            2622..3512
                     /gene="arnD"
                     /locus_tag="ACUQPX_002871"
     CDS             2622..3512
                     /gene="arnD"
                     /locus_tag="ACUQPX_002871"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HHV9057526.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            3512..5164
                     /gene="arnT"
                     /locus_tag="ACUQPX_002872"
     CDS             3512..5164
                     /gene="arnT"
                     /locus_tag="ACUQPX_002872"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HHV9057527.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            5161..5502
                     /gene="arnE"
                     /locus_tag="ACUQPX_002873"
     CDS             5161..5502
                     /gene="arnE"
                     /locus_tag="ACUQPX_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HHV9057528.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLWLGL
                     ALACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFI
                     IGGIVILGSTV"
     gene            5502..5888
                     /gene="arnF"
                     /locus_tag="ACUQPX_002874"
     CDS             5502..5888
                     /gene="arnF"
                     /locus_tag="ACUQPX_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HHV9057529.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(5882..6149)
                     /gene="pmrD"
                     /locus_tag="ACUQPX_002875"
                     /pseudo
     CDS             complement(5882..6149)
                     /gene="pmrD"
                     /locus_tag="ACUQPX_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
     gene            complement(6259..7614)
                     /gene="menE"
                     /locus_tag="ACUQPX_002876"
     CDS             complement(6259..7614)
                     /gene="menE"
                     /locus_tag="ACUQPX_002876"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HHV9057530.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVLDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(7611..8573)
                     /gene="menC"
                     /locus_tag="ACUQPX_002877"
     CDS             complement(7611..8573)
                     /gene="menC"
                     /locus_tag="ACUQPX_002877"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HHV9057531.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(8573..9430)
                     /gene="menB"
                     /locus_tag="ACUQPX_002878"
     CDS             complement(8573..9430)
                     /gene="menB"
                     /locus_tag="ACUQPX_002878"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HHV9057532.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(9445..10203)
                     /gene="menH"
                     /locus_tag="ACUQPX_002879"
     CDS             complement(9445..10203)
                     /gene="menH"
                     /locus_tag="ACUQPX_002879"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HHV9057533.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(10200..11870)
                     /gene="menD"
                     /locus_tag="ACUQPX_002880"
     CDS             complement(10200..11870)
                     /gene="menD"
                     /locus_tag="ACUQPX_002880"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HHV9057534.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFGSHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSCLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(11959..13254)
                     /gene="menF"
                     /locus_tag="ACUQPX_002881"
     CDS             complement(11959..13254)
                     /gene="menF"
                     /locus_tag="ACUQPX_002881"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HHV9057535.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAALGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(13333..13638)
                     /gene="elaB"
                     /locus_tag="ACUQPX_002882"
     CDS             complement(13333..13638)
                     /gene="elaB"
                     /locus_tag="ACUQPX_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HHV9057536.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(13693..14154)
                     /locus_tag="ACUQPX_002883"
     CDS             complement(13693..14154)
                     /locus_tag="ACUQPX_002883"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHV9057537.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMARDVIQA"
BASE COUNT         3215 a         3907 c         3661 g         3398 t
ORIGIN      
        1 agccgatgaa ggttacgacg tggtagtgac tgtacgccag aaccgccagg acagctggtt
       61 tcgtaaaaca gcttcgaaga tgattaaccg gcttattcag cgcaccactg gcaaagcgat
      121 gggtgactac ggttgtatgc tgcgcgccta tcgccgtcat attgtcgatg cgatgttgca
      181 ctgccatgaa cgcagcacct ttatcccgat tctggcgaat atcttcgccc gccgtgcaat
      241 tgaaattcca gtacatcatg ccgagcgtga gtttggtgaa tccaaataca gttttatgcg
      301 cctgattaat ttgatgtacg acctggtgac ctgccttacc acaacgccgc tacgtatgct
      361 gagtctgctc ggcagcatta ttgcgattgg aggttttagc attgcggtgc tgctggtgat
      421 tttacgcctg accttcggac cacaatgggc ggcagaaggc gtctttatgc tatttgccgt
      481 gctgtttact tttattggcg ctcagtttat cggcatggga ttactcggtg aatatatcgg
      541 caggatctac accgatgtcc gcgcccgccc ccgctatttt gttcagcaag ttatccgtcc
      601 atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg cctaccacga tatgggatgc
      661 ctcggtattg aagctctgct ggctgccggt tacgaaatta gcgccatttt tacccatacc
      721 gataatcccg gtgaaaaagc cttttatggt tcggtggctc atctggcggc ggaaagagac
      781 attccggttt atgcgccgga taacgttaat catccgctgt gggtggaacg cattgcccaa
      841 ctgtcaccag aggtgatttt ctctttttat tatcgccatc ttatttgcga cgagattttg
      901 cagctcgctc ccgcaggtgc atttaatctg cacggttcgc tgttaccaaa atatcgtggt
      961 cgcgcgccgc tgaactgggt gctggtgaac ggtgaaacgg aaacgggcgt tacattgcac
     1021 cgaatggtaa aacgtgccga tgctggggcc attgtggcgc aactgcgcgt tgccattgcg
     1081 ccagacgata tcgctattac gctgcatcat aaattgtgcc atgccgcgcg ccagctactg
     1141 gaacagacat tacccgccat taaacacggt aatattctgg aaatcgccca gcgcgaaaac
     1201 gaagccacct gttttggtcg cagaacgccg gatgacagtt tccttgaatg gcataaaccg
     1261 gcatccgtac tgcacaacat ggtacgtgcc gttgccgatc cgtggccggg tgccttcagc
     1321 tatgttggca atcagaaatt caccgtctgg tcgtcgcgtg ttcatcctca tgccagcaaa
     1381 gcacagccgg ggagcgtgat ttctgttgcg ccactgctga ttgcctgtgg cgatggcgcg
     1441 ctggaaatcg tcaccggaca ggcgggcgac ggcattacta tgcagggctc gcaattagcg
     1501 cagatgctgg gcctggtgca aggttcacgc ttgaatagcc agcctgcctg caccgcccga
     1561 cgccgtaccc gggtactcat cctcggggtg aatggcttta ttggcaacca tctgacagaa
     1621 cgcctgctgc gcgaagatca ttatgaagtt tacggtctgg atattggcag cgatgcgata
     1681 agccgttttc tgaatcatcc acattttcac tttgtcgaag gcgatatcag tattcattcc
     1741 gaatggattg agtatcacgt caaaaaatgt gatgtcgtct tgccgctggt ggcgatagcc
     1801 acgccgattg aatatacccg caacccgctg cgcgtatttg aactcgattt tgaagagaat
     1861 ctgcgcatta tccgctactg cgttaagtac cgtaagcgaa tcatcttccc gtcaacttca
     1921 gaagtttatg ggatgtgtag cgataaatac ttcgatgagg accattctaa tttaatcgtc
     1981 ggcccggtga ataaaccacg ctggatttat tcggtgtcaa aacaattact tgatcgggtg
     2041 atctgggcct atggcgaaaa agagggttta cagttcaccc tcttccgccc gtttaactgg
     2101 atgggaccac gactggataa ccttaatgca gcgcgaattg gcagctcccg cgctattacg
     2161 caactcattc tcaatctggt agaaggttca ccaattaagc taattgatgg cggaaaacaa
     2221 aaacgctgct ttactgatat ccgggacggt atcgaggcgt tgtaccgcat tatcgaaaat
     2281 gcgggaaatc gctgcgacgg cgaaattatc aacattggca atcctgagaa cgaagcgagc
     2341 attgaggaac tgggcgagat gctgctggcg agcttcgaaa aacatccgct gcgccatcat
     2401 ttcccaccgt ttgcggactt tcgcgttgtc gaaagtagca gctactacgg caaaagtagc
     2461 agctactacg gcaaaggata tcaggacgta gagcatcgta aaccgagcat ccgcaatgcc
     2521 caccgctgcc tggactggga gccgaaaatt gatatgcagg aaaccatcga cgaaacgctg
     2581 gatttcttcc tgcgcaccgt tgatcttacg gataaaccat catgaccaaa gtaggcttac
     2641 gcattgatgt cgataccttt cgtggcaccc gtgaaggcgt gccgcgtctg ctggaaatct
     2701 tgagtaagca taatattcag gccagcattt ttttcagcgt cggcccggac aatatgggcc
     2761 gccatctctg gcgactggtg aagccacagt ttttgtggaa gatgctgcgc tcaaacgcgg
     2821 catcgcttta tggctgggat attttactgg caggtacggc ctggccaggt aaagagattg
     2881 gtcatgccaa tgccgatatc attcgtgaag cggctaaaca tcacgaagtc ggcctgcacg
     2941 cctgggatca ccatgcctgg caagcccgta gcggtaactg ggatcggcaa acaatgatcg
     3001 acgatattgc acgcggtctt cgcactctgg aagagattat cggtcaaccg gtaacctgtt
     3061 ctgccgctgc gggctggcgt gccgaccaga aggtgatcga agcaaaagaa gcgttccatt
     3121 tgcgctacaa cagcgattgt cgtggggcca tgccgttccg tccactgctc gaatcaggaa
     3181 accctggcac tgcgcaaatt ccggtgacct tacccacctg ggatgaagtg attggtcggg
     3241 atgtgaaagc agaagatttt aacggttggt tactcaaccg catcctgcga gataaaggca
     3301 cgccggttta taccattcat gcagaagttg aaggctgcgc ttatcagcat aattttgtgg
     3361 atctcctcaa acgcgcagct caggaaggcg tgacattttg ccctttaagc gaactgttat
     3421 cagagacgtt gccgctcgga caagttgttc gcggaaatat tgccggacgt gaaggctggc
     3481 tgggttgcca acaaattgcg ggtagtcgct gatgaaatcg gtacgttacc ttatcggcat
     3541 cttcgcgttt attgcctgct attacctgtt accgatcagc acgcgtctgc tctggcaacc
     3601 cgatgaaacg cgttatgcgg aaatcagtcg agaaatgctg gcatccggcg actggattgt
     3661 gccccatctg ttagggctac gttatttcga aaaacccatt gccggatact ggattaacag
     3721 cattgggcaa tggctatttg gcgcgaataa ctttggtgtg cgggcaggcg ttatctttgc
     3781 gaccctgtta actgccgcgc tggtgacctg gtttactctg cgcttatggc gcgataaacg
     3841 tctggctcta ctcgccacag taatttatct ctcattgttt attgtctatg ccatcggcac
     3901 ttatgccgtg ctcgatccgt ttattgcatt ctggctggtg gcgggaatgt gcagcttctg
     3961 gctggcaatg caggcacaga cgtggaaagg caaaagcgca ggatttttac tgctgggaat
     4021 cacctgcggc atgggggtga tgaccaaagg ttttctcgcc cttgccgtgc cggtattaag
     4081 cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat ctctttattt acggctggct
     4141 ggcggttatc agttgcgtac tgacggttct cccctgggga ctggcgatag cgcagcggga
     4201 gcctgacttc tggcactatt ttttctgggt tgagcatatt caacgctttg cactagatga
     4261 tgcccaacat agagctccgt tctggtacta cgtgccggtc atcattgccg gtagcctgcc
     4321 gtggctggga ttactccccg gtgcactgta cacaggctgg aaaaaccgca agcattccgc
     4381 aaccgtctat ttgttgagct ggacgataat gccgctgctg tttttctccg tcgctaaagg
     4441 caaattgccc acctatattc tttcttgctt tgcacctctg gcaatgctgt tggcgcatta
     4501 cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg attaatggct ggattaacat
     4561 cgcttttggc gtcactggca ttattgccac atttgtgatc tccccctggg gaccaatgaa
     4621 cacgccggtg tggcaaacct tcgagagcta taaagtcttt tgtgcctggt cgattttttc
     4681 gctatgggca tttttcggct ggtacacctt aacaaacgtc gaaaagacct ggtctttcgc
     4741 cgcgctttgc ccgctggggc tggcgttgct ggtaggattt tcaattcctg acagagtcat
     4801 ggaaggaaaa catccgcaat tttttgtcga gatgacacaa gaatcacttc agccaagccg
     4861 ctatattctt actgatagcg tcggtgttgc cgcaggtctg gcatggagcc tgcaacgcga
     4921 tgacatcatc atgtatcgcc agacaggtga gttgaaatac ggccttaatt atccggatgc
     4981 gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg cttaatcaac atcgtcagga
     5041 ggggattatt acactcgtgc tttcggttga ccgcgatgaa gatatcaaca gtctcgccat
     5101 tccgcccgca gatgccatcg atcgtcagga gcgtctggtg ctgattcagt atcgtcccaa
     5161 atgatctggc taacattagt ctttgccagc ttgcttagcg ttgccgggca gttgtgtcag
     5221 aaacaggcaa cctgctttgt ggcgataaac aaacggcgca aacatatcgt gctgtggctg
     5281 tggctgggac tggcgctggc ttgtcttggt cttgccatgg tgctctggct gctggtcttg
     5341 cagaacgtac cggtaggcat tgcctacccg atgttaagtc tgaattttgt ctgggtgacg
     5401 ctggctgcag taaaactgtg gcacgaaccg gtatcgccgc gtcactggtg tggggtggcg
     5461 ttcattattg gtggcattgt gatcctcggg agtacggtgt aatgggcctg atatggggat
     5521 tattcagcgt cattattgcc tcagttgcgc aattaagcct gggttttgcg gcaagtcatc
     5581 tgccaccgat gacgcactta tgggatttta tcgcggctct gttagctttt ggcctggatg
     5641 cccggatact gttactcggg ctgctgggtt atctgctttc cgttttttgt tggtataaaa
     5701 cgctacataa acttgcgcta agcaaagcct atgccttgct cagtatgagt tatgtgctgg
     5761 tatggattgc ttcaatggta cttcccggct gggaagggac tttttcgctg aaagcactac
     5821 tgggagtagc ctgtattatg agcgggttga tgctgatttt cctgcccacg acaaaacaac
     5881 gttactgagt tttccctgcc gctgtacact gccgttccca ctcatcgggg ctataactgc
     5941 ttgccgagag gactttcacc gtatgcagct tttcacgatt aatacaataa agcgcattct
     6001 gcaaaggcga gagtaaatcc cccactttca cggcgaagtc actcttcact tcggcgatca
     6061 tttttatcgc gccgccagca tcgcagagca taagtacatg tctgttatct tgtttattgc
     6121 agcacgattt tttgtgccag ccattccatt gcattatcct gtttgctaag agttttcaca
     6181 tcaatttaca gattaacaga taaactcttt atcaacggca atggaatggc agaaatgaga
     6241 agtgaaacgg agggttaatt attgctgacg ttgcacccac tcttttagcg cctgacgtga
     6301 aattttaata ccgccgtttt tcagctccgg cggtaaagtc agccagcgca ccggttgttg
     6361 aaaacgagcc agcttatctt tcacccattc gccaagatca acggtttgct ggtcatactc
     6421 catcaccgct accggccgat gaccaaactc cttgtcggca acgggaacga taaacacctg
     6481 caaaaccgca ggatgtgcag caataacacg ctcgacttct tccggctgaa taccctcacc
     6541 gccactgaag aacagattgt ctaaacgtcc gacaatggtc agcttgccat tatgcatctc
     6601 accgcgatcg cgcgtagcgt accagccttc gtcattaacc agtgaaacca gttgcccgtt
     6661 acgccagtaa ccttctgcca tactggcagc ccgcagccac acttcattat taacgatttt
     6721 cacttcccga cccggcagcg gcaaaccaac gtctgccagg ccgtcggctt ctttcgcaca
     6781 taccgtggag gcaaactcgg tcagaccata gccgcaaaag caacgaatcc cctgctcgcg
     6841 cgcctgttcc gtcaactcga ccgggatagc cgcgccgcca agtaacaccg ctttcaggga
     6901 aacggaactg cggttaacca gcaaacgcca gagttgtgtt ggcaccagtg aagcgtgagt
     6961 acagcctgcc agcatttgct ccaatggctg tttatcacgc accgtcatcc gcgcaccagc
     7021 gtataaccag cgccacataa ttccctgacc ggagacgtga aacagcggta aagagagcaa
     7081 ccagtcatcg tgatcgccaa acggaatcag cgataacaca ccttgcgcac tggcaagatg
     7141 ggcctgataa gtatggacag cggctttcgg caaaccggta gaacccgagg tcaacgtcat
     7201 tgagcacaga cgcgtcggct gccacgcagc ggcatgtacg ccttcaacca gctgaatgtg
     7261 cagcgacgtt aatgccggaa acgtgttttc cccatccagc accagcgcaa attgcagcgt
     7321 cagattgggc agcaattctt caagcaacgg ttgcggcagc tgagggttca cgggcaacac
     7381 ccgcgccccg cattgcagta acgccagcca ggcgagcagc gtttgcggcg tattccacgc
     7441 ccgcaacatc acgccgctgc cctcaaccac accctgcgcc gcaaagcccg tagctaactc
     7501 atcgacgcga gcacaaagct cgcgccagtt gagttgctcg tcattaagac gtaaggcgat
     7561 ggcttctccc cgcacttgcc gccagtgacg ccacggccag tcagagaaga tcataacaac
     7621 tgctccagta catcagcgtc cactaacggc agtgggctcc ccggccagcg acgaacctgc
     7681 tgcgcctgca tcagatccag cgtgtccagt cccggaatgg tgtctggcgt taaccaggca
     7741 gcaatccgcg ccagttgcgt tagacctaag ctcgactcaa tggaagaact gatcaccgcc
     7801 gtcagcccca gcgcatgcgc cgcctgtacc tgctcgcgta ctttttccag actgcccgtg
     7861 agcgtgggtt tgataactac cgcgcgcacg ccctcttcag ccacaaaggc aaaatccggc
     7921 tcgcgcaggc tttcatccca ggcaatggca atgccggttt cacgggcaaa cgctcgcgaa
     7981 tcatcgcggg ttttgcacgg ctcttcgaga aacgcgatgc ggtggcgata atcagggtta
     8041 acatatttgg caaactgctg acctttcagc ggtgtccagg cgcgatttgc gtcaagacgc
     8101 aaatgcagat ccggaattgc ctccagcaac agattcacca ccatgccgtc gcgcaccgct
     8161 tcgtacaatc ccactttgac cttcgccact ttctcgcccg gcatatcggc aagtttgagg
     8221 atcagatcgt ccggatcgcc attacacagc ggtgccgcac ggtagttggc tgcttgcggc
     8281 aacgtatctg tcagttctgc caatgcacag cttacgccaa aggccacgga aggcatctgc
     8341 ggtagctcgc aatcgcctgc cagccagtta tttacccagg caagcagcac gctttgcgcc
     8401 tcttcccagg tttcctggct gaagcccggc agtggggaga tctcccccca cccttcgcgc
     8461 tcgccctcac gcaggcaaac gtacagcccg tcgcgggttt ttaaccgcct gtcgcgcaga
     8521 accacccccg cgtccatggg gatctgccag cggtatacct gcgcgctacg cattacggat
     8581 tccgtttaaa tttgctgaaa tcaggctgac gtttctggtt gaaagcgttg cgaccttcct
     8641 gaccttcttc cgtcatgtag aacagcatgg tggcgttgcc cgccagctcc tgcagccccg
     8701 cctgcccgtc acagtcggcg ttcagcgcag ctttcaggca gcgcagcgcc atcgggctgt
     8761 tttgcagcat ttcgcggcac caacggacgg tttctttttc cagatccgcc agcggtacca
     8821 cggtgttgac aaggcccata tccagcgcct gttttgcgtc gtactgacgg cacaggaacc
     8881 agatttctcg cgcttttttc tgcccgacga tacgagccat gtaggaagcg ccccagccgc
     8941 cgtcgaagga accgactttc gggccagtct gaccgaagat ggcattatct gctgcgatag
     9001 tcaggtcgca catcatgtgc agaacgtgac caccgccgat ggagtagcca gccaccatcg
     9061 cgacaaccgg tttcggacag gtacggatct gacgctggaa gtccagcacg ttcaggtgat
     9121 gtacgccgga atcatcttta tagccgccgt aatcaccacg cactttctgg tcaccaccgg
     9181 agcagaacgc tttatcgcct gcgccagtca gaatgatcac gccgatattg tcgtcataac
     9241 gcgcatctgc cagtgcctgg atcatctctt ttaccgtcag aggacggaag gcattgcgca
     9301 cctgcggacg attaatggtg atttttgcga taccgtcggt ggatttttca taacgaatgt
     9361 cctcgaaacc ttcggagcag tcgtgccatt caaccggtgc gtaaggcatt gcttcatcag
     9421 gataaatcat attgtgtcct ttagtcagaa acgcaagatc tgcgccagac ttgcgattac
     9481 gccagcggga ttttcccgat gcgcgttatg tccggcgcga ggaatgacat ggcagtcggc
     9541 agccagttcc gccgccaggg cgcggaattt gctgtcacgt tcaccacata aataataaaa
     9601 cgcaaatgtg cgggcgctaa ggttagcacg taaatcaggc tggacggcga gagaagtcgc
     9661 ctccagcatg gcggcaaggg ttgcgccatt attgttgctg cgcagcgcca ccagctcccg
     9721 gcgttgatcg tcattgagcg aggcaaaaac aggctgttga taccagtcgg caaataccgc
     9781 cgttaacggt tctgtgcgaa aacgctgcgc ccattggcga tcggaacgct gacgttccgc
     9841 acgttgttca gcattttgca gccccggatg cccgccttcg acaataaccc cacaaagccc
     9901 cgccagcccc tggcaagccg ccatcatcgc cacccgtccg ccaagcgagt accccaccag
     9961 ccagaagtca aggatattgt aactaaccaa ggttttacgc agtaagtcgg tgacatcatc
    10021 aaatccatcg acgctaatcg ccgccgaacc accatgacct gggagatcaa catacaaccg
    10081 tgagtagtcg gcaaacgcct cgcccacttc ttgccattcg tggcaatcgc cggaaaaacc
    10141 gtgcaaaaac accagccagg gtaaacctgg ttttccgtgt tttgcctgcg cgtgcaggat
    10201 cataaatggc ttacctgcgc cagaagttgc tggagcgttt gcgcaccatc ggtgtcgtta
    10261 accaccattt caatcaccgt ggtggttggc gtgcgccagg cgtcggcaaa tgccgtttca
    10321 agttcctgcc agttttgcgg acgatgatat ttcagctcga acatcgcggc ggcgtgctca
    10381 aaatggacgt tttgcggcat cagatagaaa cgctcacgct cgctttgcgg cgttggcaac
    10441 agcgagaaaa tttgcccgcc gttgttgttc accacaatta ataccagcgg cgcagaaacc
    10501 tgacgcaata acgccagcgc gttgagatcg taaagtgcgg agagatcgcc cacaatcgcc
    10561 agcgtcggtt tgccgcttgc ccgctgaacg cctgcggcgg tcgaaagcag tccgtcgata
    10621 ccgctggccc ctcggttgct gtacaccggg taacctgccg gaagttgcga aagcgcatca
    10681 atcagacgta ccaccaggct gttaccaaca aacaattgcc cctgttcagg cagatagtcg
    10741 ctgatgcgat gcgccagttg cgcttcgcca aacgcatcac ggcgggcaat aaccgcctgc
    10801 attgcctgtt ccgccaggcg cgggatttca acgcaccagg gctggcgttt ttctgccgga
    10861 tgcagctcca gccagtcggc aatattggca attaagcgac gtccgcgatg gtgtgccgga
    10921 tcaagtcgcc cttcaatgtc atcaacaatc cagtactctt ctggttcaca gcttgcctgc
    10981 cattgcagga gccgtttccc cgtcaggcag cttcccagtt gcaccacaat ttgcgcctgc
    11041 tgcagctcgc tggtcgcttt ggcattgcct aaccaaagat cggcacacgg cagcggctgc
    11101 ccggtttgtg acagcacatc gccaatcagc ggccagccaa gagtttgcgc ccacagggca
    11161 acttttttgc cctcttccgc actcatgcgc ccggcaacca ccacgccgcg cttttgtcgc
    11221 cagaagaacc agtcgcgctg tttttcactt tccagacgag gcgcttcacg cagccacggt
    11281 ttgtcgtcct gccaccagtc acccagacgc tgttgccagc taagcccggt atcgtccatt
    11341 tcgccataca gcggttcagc aaacgggcag ttgatatgga ctccccccgc atgaagcgta
    11401 ccgagagcgt ggtcgatggt agaaaccagc caacgtgcgg ggatatcctg ggtcgggcgc
    11461 ggcaatgaaa tactgtgcgt ggggtgagag ccgaacattc ccggctggcg aattgcctga
    11521 ttcgcgccgc agtcaattag ctccggcggg cgatcggcgg ttaagagaat cagtttttct
    11581 ccggttaacc cggcttcaat cagtgccgga tagagatttg ccaccgccgt gccggaggtc
    11641 acaatcaccg ccaccggctg cttgctgact ttcgccagcc ccagcgccag atgccccaac
    11701 ccacgctcat cgaaatgggt gtggtgaatg aatgcggaat tctccgccgc cgctaacgtt
    11761 aacggtgtag aacgcgagcc tggggcgata cagatgtgtc tgacgccgtg acgcgttaat
    11821 gcttccagaa tgaccgccgc ccagcgtcgg ttaaatgcgc ttactgacat gagtttgtcc
    11881 ggtatcaata ttgcggctaa gtataaggag ctacaaaaat agattattga tatgaatcgg
    11941 taatgatgcg actcattact attccatttg taataaagta cgcagccctg ccgctttgtt
    12001 gtcgatttcc tgccactctt gctcggggtc ggaaccacgg acaatgcccg cgccagcata
    12061 taatcgcacg acattgccgc taatttttgc tgagcgcagg gaaacgcaga attcgctttg
    12121 ttgtaatgag agatagcccg cagaaccggc gtaccattct cgggtgaacg gttcgtgacg
    12181 ggcgataaac tgtcgcgcca gatcgcgcgg taagccagca actgctgccg ttggctgcaa
    12241 ctgatgtaaa cagatcacat catccgcttt gttgagtgaa gtccagatac agcggcgaag
    12301 atgctgcact ttacgcagac gcagtacctg cggcggtaaa acatccagcg tctgggtatc
    12361 ggcctgtaat cgttggcaga tatcttccac caccagcatg ttctcgcgct ggtttttatc
    12421 atccgccatc agccactctc ctaactgctg cgcctgctta tcatcaggat gatttgctac
    12481 tgttcccgcc agcgcttcag tacgcagcgc tttgtcacgc cgccgccata accgttccgg
    12541 tgaagagcca agaaaagcat tttcgccatc aaaggccatg taaaaatggt agcaattcag
    12601 attcagtcga cgactggcag ccatcatcgc cgccgcgttg accggacttg cgaaatgcag
    12661 gtcagttgcc cgagcgagca ccactttgtc gagctcacct tcggcgatgg ttttcgttgc
    12721 cagttcgatt aattgcgtcc agcccgtttt gtccggccag tgttgttctc gcgtggtggt
    12781 taaatgtaac ccaggcaagg gcttgatact caccagtgtg gcgataaatt cttttgcctg
    12841 aatcgcatcg tgctgaaggg agctttcgct gaatagcgtc agccgcagcg tggctttacc
    12901 gccacagcgt cgccattcca ggcggggtaa aagtaaattg ccctgcgacg ggtcaaaagc
    12961 attcagcccc caaatgcgta agtcggcgtg ttccgggtgc tggcgaagaa aacgttgtgc
    13021 ctggtccaac gacgtaaaac gggtaatcgc gcccagggcg gcagcttctt catcaccatt
    13081 acgttgttgc cagtagaatt gcgggtacgt ttgctgactg gccagccagc tcaaggcatc
    13141 aaaagcgtct ttgagaggga aaggaatatc gataacccga atgccgggtg tcgccggaat
    13201 ctcttgcgac aaatggcgca gtagattttc cagcgccgta gtaagtgatt gcacgcggac
    13261 ctctccctgt taaaagcctc acattatacg gggtactaca aaaaaaatgc agtaccccgg
    13321 tgtagggagg ttttaacggc gtgccagcaa cagtcctagt accagcccga cggccgcgcc
    13381 cacaccaatt ccttgccagg gtttttcgtg gacgtagtca tcagcacgat aaacagcctg
    13441 cttcgcccga tagtaataac tgtctgaagc ctggctaacc cgttttttca catcatccag
    13501 cgctttttct gcacgcgctt tcagctcaac atatttctga tcggcgggat cgccagagga
    13561 gcggagcacc tcttccagtg tttcactaag cagcgtcagg tcgtcatcga tacgtgtatc
    13621 accaaactga ttagacattc tcgttctcca tttgcgtaaa acctgtcact aactatagac
    13681 aagagttgtt gattacgcct gaattacatc gcgtgccatc ccaatgtgtg ggataccatc
    13741 ttcttcatag acctccgtca cagggataaa accgaaactc tggtagaagt tttgcagatg
    13801 cgcctgcgcc ccgaggtaaa caggcttatc aggccagtga tgcgtacatg tttcgagtgt
    13861 tttactcatc agctgctgcc ccactttttc accacgcaac gcttcactga caatcacccg
    13921 acctataacg accggctcaa gatcatcatc acttttcaga atcctcgcat acgccaccag
    13981 ttcatcattt ttccaaccga gaatatggcg attatccccc gtcaaatcat caccatcgat
    14041 atcctgataa gggcaattct gctcgacaac aaacaccgcg caacgcagtt gtaataaggc
    14101 ataaagctgg gaaaccgata attcagagtg atgcagatct tgccattcaa tcataatgct
    14161 ctccttttag atgtatgcct t
//
LOCUS       DBKEKI010000095        13882 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.095, whole genome shotgun sequence.
ACCESSION   DBKEKI010000095 DBKEKI010000000
VERSION     DBKEKI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.095"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            70..1422
                     /gene="celF"
                     /locus_tag="ACUQPX_002884"
                     /gene_synonym="chbF"
     CDS             70..1422
                     /gene="celF"
                     /locus_tag="ACUQPX_002884"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HHV9057538.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNSAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLICSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1435..2193
                     /gene="chbG"
                     /locus_tag="ACUQPX_002885"
     CDS             1435..2193
                     /gene="chbG"
                     /locus_tag="ACUQPX_002885"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HHV9057539.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2240..3160)
                     /locus_tag="ACUQPX_002886"
     CDS             complement(2240..3160)
                     /locus_tag="ACUQPX_002886"
                     /inference="COORDINATES: protein motif:HMM:NF036542.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-related domain-containing protein"
                     /protein_id="HHV9057540.1"
                     /translation="MHRMGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEG
                     NKVRERSPSFGEYYSHPRLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAH
                     IDLTLAQAVAKNLGIELTDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILL
                     NDEVRSADLLAILKALKAKGVHAKLLYSRVGEVTADDGTVLPIAATFAGAPSLTVDAV
                     IVPCGNIADIADNGDANYYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADS
                     ADGSFMDELLTLMAEHRVWSRIPKIDKIPA"
     gene            complement(3164..4491)
                     /locus_tag="ACUQPX_002887"
                     /pseudo
     CDS             complement(3164..4491)
                     /locus_tag="ACUQPX_002887"
                     /inference="COORDINATES: protein motif:HMM:NF012425.6"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
     gene            4674..4937
                     /gene="cedA"
                     /locus_tag="ACUQPX_002888"
     CDS             4674..4937
                     /gene="cedA"
                     /locus_tag="ACUQPX_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HHV9057541.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5226..6030
                     /locus_tag="ACUQPX_002889"
                     /pseudo
     CDS             5226..6030
                     /locus_tag="ACUQPX_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(6034..7425)
                     /gene="tcyP"
                     /locus_tag="ACUQPX_002890"
     CDS             complement(6034..7425)
                     /gene="tcyP"
                     /locus_tag="ACUQPX_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HHV9057542.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     DSFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSSSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(7558..8148)
                     /locus_tag="ACUQPX_002891"
     CDS             complement(7558..8148)
                     /locus_tag="ACUQPX_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HHV9057543.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(8311..8979)
                     /gene="hxpB"
                     /locus_tag="ACUQPX_002892"
     CDS             complement(8311..8979)
                     /gene="hxpB"
                     /locus_tag="ACUQPX_002892"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HHV9057544.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     EQPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            9126..9662
                     /locus_tag="ACUQPX_002893"
     CDS             9126..9662
                     /locus_tag="ACUQPX_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HHV9057545.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLRESVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(9703..10563)
                     /locus_tag="ACUQPX_002894"
     CDS             complement(9703..10563)
                     /locus_tag="ACUQPX_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HHV9057546.1"
                     /translation="MWQAIIRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPHRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(10669..10959)
                     /gene="ghoS"
                     /locus_tag="ACUQPX_002895"
     CDS             complement(10669..10959)
                     /gene="ghoS"
                     /locus_tag="ACUQPX_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HHV9057547.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(11060..11989)
                     /gene="pfkB"
                     /locus_tag="ACUQPX_002896"
     CDS             complement(11060..11989)
                     /gene="pfkB"
                     /locus_tag="ACUQPX_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HHV9057548.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            12276..13034
                     /locus_tag="ACUQPX_002897"
     CDS             12276..13034
                     /locus_tag="ACUQPX_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HHV9057549.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(13087..13194)
                     /gene="yniD"
                     /locus_tag="ACUQPX_002898"
     CDS             complement(13087..13194)
                     /gene="yniD"
                     /locus_tag="ACUQPX_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HHV9057550.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(13366..>13882)
                     /gene="espL1"
                     /locus_tag="ACUQPX_002899"
     CDS             complement(13366..>13882)
                     /gene="espL1"
                     /locus_tag="ACUQPX_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HHV9057551.1"
                     /translation="IIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMEN
                     NHPLCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYI
                     STLGAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHKHYKTVETYYAAINVISQSL
                     SFSADEIKTYL"
BASE COUNT         3574 a         3200 c         3427 g         3681 t
ORIGIN      
        1 tgctggatta tttatgtccg ggggggcaga tatgccagat atcagtattc tgtactgaag
       61 ggagaaatta tgagccagaa attaaaagtc gtcactattg gtggcgggag cagttatacc
      121 ccggagttac tggaaggatt tattaagcgt tatcacgaat tgccggtcag cgaattatgg
      181 ctggtggatg tcgagggtgg caaagctaaa ctggatatta ttttcgatct ctgccaacgg
      241 atgattgata acgctggcgt cccgatgaag ctttataaaa cgctggatcg ccgcgaagca
      301 ttgaaagatg ctgatttcgt tactacccaa ctgcgcgttg gccaattacc ggcgcgtgaa
      361 ctggatgaac gtattccatt aagtcatggt tatcttggtc aggaaaccaa cagcgcgggc
      421 ggtttgttta aaggtctgcg taccattccg gtgatttttg acatcgtaaa agatgtcgaa
      481 gaactttgtc cgaatgcatg ggtgattaac ttcactaacc cggcgggaat ggttactgaa
      541 gccgtttatc gtcataccgg atttaaacgc tttatcggcg tgtgtaatat tccgatcggc
      601 atgaagatgt ttattcacga tgttctgatg ctgaaagaca gcgatgattt atctatcgat
      661 ttgtttggcc tcaaccatat ggtgttcatt aaggatgtgc tggtaaatgg caagtcgcgc
      721 tttgccgaat tgcttgatgg tgtggcatcc gggcagttaa aagcatctgg cgttaaaaat
      781 attttcgatc tgccatttag cgaaggctta atttgttctc tgaatctgtt gccgtgttct
      841 tatttgcttt attacttcaa gcaaaaagag atgctggcta ttgaaatggg cgaatactac
      901 aaaggcggcg cacgagcgca ggtagtacag aaagtcgaga aacaactttt tgagctgtat
      961 aaaaacccgg agttgaaagt taagccgaaa gaactggaac agcgcggtgg ggcttattac
     1021 tctgatgcag cgtgcgaagt gatcaacgct atctacaacg acaagcaagc tgaacattac
     1081 gttaatatcc cgcatcatgg gcatattgat aatattccgg cagactgggc ggtagaaatg
     1141 acctgtaagc tggggcgcga tggcgcgacg ccacatcctc gcattacgca tttcgatgat
     1201 aaagtgatgg ggctgattca caccattaaa ggcttcgaga ttgctgccag caacgccgca
     1261 cttagcggag aatttaacga tgtgttactg gcgctaaacc ttagtccgtt ggtgcattcc
     1321 gatcgcgatg ctgagctgct ggcacgcgag atgattctgg cgcacgagaa atggctgcca
     1381 aattttgccg actgcatcgc agagcttaaa aaagcacatt aaccgaggct gattatggaa
     1441 cgcttactga ttgttaatgc cgatgatttt ggcttaagca aaggtcagaa ctacggcatt
     1501 atcgaggcct gtcgcaatgg gattgtcaca tcgacgacgg cgctggtgaa tgggcaggct
     1561 attgaccatg cggtgcagtt gagtcgtgat gaaccgagtc tggccatagg gatgcacttt
     1621 gtccttacta tgggcaagcc actgacagct atgccggggt taacccgcga tggtgtgctg
     1681 ggaaaatgga tctggcagtt ggcagaagaa gatgctttac cgctggaaga aattactcag
     1741 gagcttgcca gtcagtattt gcgtttcatt gagctatttg gacgcaaacc tacgcatctt
     1801 gatagccatc atcatgtgca tatgttcccg cagattttcc cgattgttgc caggtttgcg
     1861 gctgaagagg ggattgcgtt gcgcatcgac cgtcagccgc tatctaacgc tggtgattta
     1921 ccggctaatc tgcgcagttc gcagggattc agtagcgcgt tttatggtga agagattagc
     1981 gaggcactgt tcctgcaagt gctggatgac gcaagccatc ggggtgatcc ttcgctggag
     2041 gtgatgtgtc atccggcgtt tatcgataat actatccgtc agagtgctta ctgtttccca
     2101 cggttaacgg agctggaagt gctgacatca gcgtcgttga aatatgcaat tgctgagcgt
     2161 ggttatcggt tgggtagtta tcttgatgtg taagtaaggt ggtaaggtca ctccccaggg
     2221 gacgtgacct tatccatatt tacgccggaa ttttgtcaat cttaggaatg cgtgaccaca
     2281 cgcggtgttc tgccatcagc gttaacagtt catccataaa actaccgtcg gcgctgtcag
     2341 cttccacaat cccttcttca ccctggtcag ctaccttgat tgttgcttta aacttgcgcg
     2401 cgtctcccgc cagcgcaatc ggtttaaggt gtttgtaggc ttccatcaga tagtagttgg
     2461 catcgccgtt gtcagcgata tccgcgatat tgccgcaagg gacaatgacc gcatcgaccg
     2521 tcagcgaagg tgcaccggca aaggtagcgg ctataggcaa caccgtaccg tcatccgcag
     2581 tcacttcacc cactcgggag tagagcagtt tggcatgaac gcctttggcc ttcagggcct
     2641 tgagaatggc cagaaggtct gccgatctca cttcatcatt aagtaaaatc gctaccacgc
     2701 gacctttcac atcaccgtca ggaatggcgt acaaacttaa ggatggatcc tttttcagac
     2761 cgttgacgtc cggaggtggg gtgatattca gctggtcgtc agtcagttcg ataccgagat
     2821 ttttcgccac cgcctgggcc agagtgagat caatatgcgc cagctggtca acaacgcgct
     2881 cacgaatata cggacgaacg actttgctta actcaaaact gaaaccatcg acaatatggc
     2941 gctgctcgaa tggcgtctga cttagccaga acagacgcgg atgggaataa tattcgccaa
     3001 acgatgggct gcgctcgcga actttattgc cttccacgcg ctcctggtat gattcaaaac
     3061 cgccgcgttt cggccccggt ggtgtttcgc gcggccagtt atcgttgatc gagttcggtt
     3121 cataattcgc cgggttagtg tcgatcccca tacgatgcat gccgtcacgc tggaaattat
     3181 ggtaagggca ggtcgggcgg ttaatcggaa tctcatggaa attcggccca ccaagacgac
     3241 tgatttgtgt atcggtatag gagaacaaac gtccctgcaa caggggatcg ttggtgaagt
     3301 ccagaccagg aacaatatga cctggatgga atgctgcttg ttcgttttcg gcaaagaagt
     3361 tatccggatt gcgattgagc accattttgc cgacacgctg aacgggcacc agttcttccg
     3421 ggatcagttt ggttggatcg agaagatcga agtcgaactt gaattcatct tcttcaggaa
     3481 tcaactggaa gcccagttcg tattccggaa aatcgcctgc ttcaatggct tcccacaact
     3541 cgcggcggtg gaagtccggg tcacgtccgg tgagtcatcc caaacgagtg aggctttacc
     3601 tgccagtggt ttccagtgga aacgtacaaa cgttgccttc ccttcggcat taatcaggcg
     3661 gaaggtgtga ataccgaagc cttccatggt gcggtaactg cgggggatgc cgcgatccga
     3721 catcgcccac atcacgttgt gcagagtttc aggttgcaga gaaacataat cccagaaagt
     3781 atcgtgggca ctttgccctt gtggaattgc ccagtgcggt tctggtttta ccgcatgaac
     3841 aaaatcgggg aatttatgcg catcctggat aaagaagatt ggcgtgttat tgccaacgag
     3901 gtcaaaaata ccctcttcag tatagaactt ggtggcaaaa ccacggatat cacgcacggt
     3961 atcggcagag ccagcgccac cctgaacggt agagaaccta acaaatactg gggtgatttt
     4021 gttcggatct gagaggaaat ccgctttggt gatatcgctt aagcttttat atggctggaa
     4081 ataaccgtga gctgctgatc cgcgtgcatg aacaatacgt tccgggatgc gctcatggtc
     4141 aaagtgggtg attttctcgc gcagaataaa atcttccagc agcgttggac cacggttacc
     4201 ggcacgcagt gagttttgat catcggcgat gcgcacgccc tgattagtgg tcagcgcata
     4261 attttcactg cctttgcgta cgtcttccag agaattaagt ttttcgttac gtgtatcagg
     4321 ggctttcagg ctcccagggg cggtaggttg tgcacctggc ggtgttggtt cagccgctgg
     4381 acgatgagat ccgtcctcag gtgccagtga gtccatcccc ggtttcgctt cgctggaatc
     4441 gtgtagtggt gactggtgct gatgtgggtt cttttcgtta tgttgcgaca ttgaactcgt
     4501 ctccttaatt tattactgaa agggccgctg ttttggtcaa aaaactctct aactatagac
     4561 caccagccag atcccgcttc ggagactatt ctggaaacgg tgttatgaaa tacacgcaaa
     4621 gcgggacgta caggggctaa atcggctaaa ctacagtttt tctaattttt aatatgcgtt
     4681 tagtgaagcc agtaatgaag aaaccgctcc gccagcaaaa ccgccagatt attagctatg
     4741 tcccacgcac ggaacccgcg ccgccagaac atgcgataaa gatggattcg tttcgtgatg
     4801 tctggatgct gcgtggcaaa tatgttgcgt ttgtactgat gggagagtca tttctgcgct
     4861 caccggcgtt tactgtgcct gaatctgccc aacgttgggc aaatcagatc cgccaggaag
     4921 gggaagtgac tgagtaagga ttacatacct cccggttttg ccgggagtta catgacctga
     4981 aattctgcat ttcgttgcta cattcattca aaccacctgt aacaaaaagc tgcctttgac
     5041 gagctattgc tataaataag tgatcttttt tctttaattg tttatttatt ttaaaatggt
     5101 taagttttgc taatcatgat gagctatttt tgatagttca tcctgtggcg tcgctcttaa
     5161 acttatattg taaatgactt agatttaaat aagataataa ttttgttaac aatatgaggc
     5221 gggagatgtt ttctacaaac ttaatacaga gtaattatgg tgatttaaat attaaaagcc
     5281 tggctttcga ctcttttaaa gaaaggcttg agtctactat ggcggcactc actttcttta
     5341 tatcaacggg tcaatgtgac tgcgatgaaa tagcagagtc gaacttcaat cgtatgattg
     5401 cctatatgtc taacattaac tatgatgcct caaagctagg agcgccagca cttagttttg
     5461 atacgtatct gcaggacaat gttaaatata gagtgataat taataatctt tatggcagtg
     5521 agtttcggat aagagatatt aatgagaatt ttgttgggat ggattttacc tgtgaattcc
     5581 gacccgaaaa aatgacgaat ttgatcaata taaaaaatag gttggttatt cattatttaa
     5641 ggactatata ctatgaaaaa aattatatac atcctgccgg gagcattttt gctgcgattc
     5701 aaaaaaaatg agagtctcct aaagtttcct tcgataatat caatgcttaa tatcaatctt
     5761 ctttttaatc cactaaatct tccgggaatg ggaagtggtg tgcttgaaga tattatgagt
     5821 attccagata gttcacttag aaaacgtctc gggtatgagg ttttaagctt ctcactacag
     5881 gcacattcat tgagccagga atgtattgat aagttggaca tttttttcgc tgacgatctg
     5941 tttaaatatg agtcagtatg catcgccgcg atggaacatt taaaaagtaa agcaacggcc
     6001 cctatacaaa acggaccgtt gccagcataa gaattaacgg tgtgccagtt cggcgtcgtc
     6061 ttcgctatcc agaatggctt tatcggtttg cttcagccac tggctggtca gcgtgccagc
     6121 tgtcatcgag ctactaacgt ttagcgccgt acggcccatg tcgataagcg gttcaacgga
     6181 gattaacagc gccaccaggg ttactggcag gcccatcgca ggcagcacaa tcagtgcggc
     6241 gaaagttgca ccaccaccga caccggcaac gcctgcggaa ctaacggtaa caataccgac
     6301 cagcgtcgca atccacatcg ggtccagcgg gttaatgcca accgtaggcg caaccatcac
     6361 cgccagcatt gccggataca aaccggcgca gccgttctga ccaatggttg caccgaaaga
     6421 gtcggcgaaa ctggcgatgg attcaggaac gcccagacga cgcgtttgtg cttccacatt
     6481 cagtgggata gacgcagcac tggaacggct ggtaaaggca aacgtcagca caggccatac
     6541 cttacggaag tacttcagcg gactcacgcc attaatgccc agcagaatgc catgcactgc
     6601 aaacataatc agcagaccga ggtaggacgc gacaacgaaa cttcccagtt tgatgatgtc
     6661 ttgcaagtta gaacctgcaa ccactttggt cattagagcc agaacgccgt aaggggtcaa
     6721 ctgcatgaca aggcgaacca gtttcatcac ccagctttgt agggtatcga tagcggttaa
     6781 gacgcgttca cctttcggcg catcatcctt cagcagtttc aatgcagcta cgccgaggaa
     6841 tgcggcaaaa attaccacgc tgataattga cgtcggattg gctccggtaa gatcggcaaa
     6901 cgggtttttc gggataaagg acaagaccag ctgcggaacg ctcagatcag agactttacc
     6961 aacatagtta ctttcaatgg cgttcagacg tgcagtttct gcaccaccct gaaccagacc
     7021 ttcagccgtc aaaccaaaca ggttggtgac cagcacaccg accagcgccg caatcagcgt
     7081 ggtaaacaaa agcgtaccga tggtcagaaa actgattttg cctaactgag atgcgttatg
     7141 cagacgggca accgcgctca gaatagaggc gaagactaac ggcataacga tcatttgcag
     7201 cagttgaaca tagccgttac caacgatgtt aaaccactgt acagaatctt taagtacctg
     7261 gctgtcagaa ccataaatgg tatgcagggc aaggccaaaa accacaccca tcaccagacc
     7321 caccagcact tttttcgcca gactccactg tttatggcgg gtctgagcca gcgcaaacag
     7381 cagtacaacg aacaccacga tgttcgcaat taatggaaag ttcatccccg ttctcctgat
     7441 cttattattt ctatcactgc gtagcagtca tagtgttggc gcaagattaa cagaactgtg
     7501 agctggctct tatattcaaa tggaattgct tattccagaa aggtgatttt tgccttttta
     7561 gtattcaacc tgatgcttaa taagccgatg aaactggatc tgcaaggtat tgatcatttg
     7621 cgatgaccaa cgaacagcgc tgttctcggg taatgaatgg ggcatccata ccgaccagac
     7681 aaataatgcc atgcagataa aacgttccag ttggttgccc ttttggggaa ccaggatagg
     7741 caagcggaaa cgccagcggc atggccagag caggggaaca ccggcgggtg tcagcatatc
     7801 ggcaagtatg tggctcaaat aacccagcac cattccttgt agcgcatcag ccggaatgaa
     7861 ccagccttcc ggaaccttaa ggtaaaaggt tgccagcagc gcaaataccg ccagcagact
     7921 gtgggtaaaa ccacgatgcc caaaagcgcg ggcgatcggt tttgatatcc attttaatcg
     7981 ctgcccaaga aacgactttg ggtgatcgat gtccggtaac aaacacgtca ggattgcgga
     8041 agggacaata tgccaccagt caccctgtgc cagcacgggc gtcagctcgg catttttggc
     8101 aaataccgca caagcaatag aaaagagaag gtgaccttcc gccgtcatga taaaactcac
     8161 taaactgtcg atacgtacag tataggattt ttatacagtg cacggaagtg gtgacggtgt
     8221 aactctttct cttaaattga cgattaatcc gtcagttgaa tgcaaattca gataaagaac
     8281 caggggcagc gtgatcactg cccctggtca ttaaccgaga aggtcttttg cggtgagttc
     8341 tgtcagcgat gagagtttga cgtttgctaa tacaaaacgt ggatcatttt gcgcttctgg
     8401 cgcaggaacg acgatggaac gcatgcgggc tgctttagaa gcgatcatgc catttaccga
     8461 gtcttccagc gccacgcagg tcagtgggtc aacgcccagt tttgctgcgc agtcgagata
     8521 tacttgcgga tgcggcttgc tgtaaggcag tttttcagcc gaggcgaggg catcgaaact
     8581 gtcgcgtaag tcaaacatgg tcaacacttt ttccagcata tgtagtggtg acgcggaggc
     8641 cagtcccacc aataaacctt gttctttgca taacgcaaca gcttcgcgca cgcctggtaa
     8701 taatggacgt gtctcttcaa ccagtgaaat ggcacgggca ataacccgtt ctactacttc
     8761 ctgacggctt ggcccattcc acggttgccg ggcgtaccaa agatcgacca ccatatcgat
     8821 gcgtaaacct aaggtgtccg gctgctcgtt acgacgggag atatccaccc ccaggcttgc
     8881 catcacatcc agttcggctc gatcccataa aggttctgag tcgataagta atccatccat
     8941 atcaaaaatt gcagcaagaa tctgacgcgg ggttgacatt acaacctctc ctttagagca
     9001 ttatgtgtaa ggtattaagc cgtgcttatg attaagcgcc tatcatatcg cctttaaaga
     9061 atgggcgaaa acagatatct acaggtaaac taagtgaaac gacgcgtctt aatcaaggag
     9121 aactcatgac gtatcaacaa gctggacgca ttgctgtttt gaaaaggatt ttgggttggg
     9181 tgatttttat acctgcattg atctctacat tgatttcttt gctgaaattt atgaataccc
     9241 ggcaggaaaa ccaggaaggc attaatgcgg tcatgctcga ctttgctcat gtcatgatcg
     9301 atatgatgca ggcgaatacg ccttttttga atctgttctg gtataactcc ccgaccccta
     9361 attttaatgg tggtgtgaac gttatgttct gggtgatttt catcctgatt tttgtcggac
     9421 tggcgttgca ggattccggt gcccggatga gtcgccaggc gcgcttcttg cgggaaagtg
     9481 ttgaagatca actcattctg gaaaaagcca aaggggaaga ggggctaacg cgtgaacaaa
     9541 ttgagtcccg tattgttgtt ccgcaccata ctattttcct gcagtttttc tcactatata
     9601 tcctgccggt tatttgtatt gctgcaggtt atgtgttctt ttctctgctt gggtttattt
     9661 aatcaaaagg ccgcacggag cggccattat cctcaaccca tatcatgctg ctaataatct
     9721 atccaatgac tgctgagcaa taaccaaatg ctgaccgcca aataatcttg cacgatttaa
     9781 cagcgtgtag agttggtaaa ccggttgacg ttcaaggaaa tcggcaggta gcggggatac
     9841 tgactgatag ccgtcataga tttgtggcgg ctgttcagta tgcagcggta acatcgctag
     9901 gtcgcactct cggtcacccc agtagcaggc agggtcgaaa atgtacgggc catccggacc
     9961 cagcgcacag ttgccggacc ataaatcgcc gtgcaacaga gaaggctgcg gttgatgcga
    10021 ggccagacgc tgctgaatat gctcgacgat ggcgtcgata ttgccgaaag cgatcccttt
    10081 ctctgctgcc agttccaact gccagccaat ccgttgttca gcaaaaaacg ttgaccagcg
    10141 acgttgccag gtgttgggct gtggagttgt ggagagcgag ttatcaaaat cgaggccaaa
    10201 ttgtggttgg tcactccatt gatgtaaacg cgcaatttgc tgaccaagaa taaatgcgct
    10261 atgcgcatcc agcggacgat gtgggagata atccatcacc agaaaactgt agtcacggtc
    10321 agcgcctact gcccaaacct taggcacggt gacggtttta ctacgcgaca gtaactccag
    10381 ttggtcggct tcggcggtaa aaccgggaag cagttccctt tcatcacatt tgacgaaaaa
    10441 gtcatgtcct gcatagcgca aatgccatgc ggcatggact tctccgccag gcagttcatt
    10501 acgcagttcg atttcgcctt cacctaactg ctcgctcaaa agacgaatga ttgcctgcca
    10561 catgatgtct ctcccatgtt gtctgccaga tcataaggtt agcgcaagaa tgcggtgtaa
    10621 aaacacgagc taacgcacac cacagactga ttattgccca aaaaccattt attttctgtt
    10681 gctattccat tcctcccagg tcgtgatggt gatttcagga tctttgccgg ttgcactttg
    10741 ggtaagcccc gaaaccagtt ctctaatttc ttcttcactt tgggtactga taagtccaaa
    10801 agtgttagtc cccagctcat ggatatttcc ctcatcatcc gtcatggtga gcaaaaaacc
    10861 gtcgcgagtc aggtaattat tcaactcgtt aatttcagtc aatgtatcct catgcaacat
    10921 gacggttatg acataacgga caagatcgcc actggccata gttcacccct ttgttatcac
    10981 atgacttttg agcatagtcg gagaaacgcg ttgccgacag gttggtggtg attcccccaa
    11041 tgctggggga atgtttttgt tagcgggaaa ggtaagcgta aattttttgc gtatcgtcat
    11101 gggagcacag acgtgttccc tgattgagtg tggctgcact ccccgcagct acgccaaaac
    11161 ggaccatctc ttcaagagag gcattttctg ccagtttcag tgtcatcgcg ccgaccatgc
    11221 tgtcaccagc gccaacggta ctctggcttt tcaccggtgg tggcaccacc tgaatgcagt
    11281 tttcactatc aacacccagc gctccttgtg gaccaaggga aacgacaacc cgtttggcct
    11341 tgccgctatt aacgatttcc tgcgcggctt tgcggacatc gtctggctgg gtgagttcgc
    11401 gattcaccag cgcactgagt tctttttggt taggcttaac caactcgatg ttaccaatcg
    11461 ccagtgctgc acttaacgct tcgccagaac tgtcgatgat gcagcggatc ccttggtttt
    11521 gcgcagcgga aatcagttgg gttaattttt ccagcttcac acctggcggc aggcttccgc
    11581 ttatgaccag gatggccccg gattcaattt ccagaacttg ctcttcaagc tggcgaaact
    11641 catcttcatt taatgccgcg cctggcataa caaaacgata ctgttcaccg cttgcttcca
    11701 catgtacgtg taaattctgc cgggtccagt ctttggcttc tacagtagcg acggggacat
    11761 tttcatccgc caacagtgaa accaggtgtt cgccggtcgc gccacccgcc gggaagatcg
    11821 ctgtggcact gcctccaaga tgggcaatgg cgcgggcgac gttgatgccg ccgcccccgg
    11881 gttcgaacac cggtgcggta cagcgcagct ttccttcggg ataaatttgc ggggtaattg
    11941 ttgcgctatc gagagagggc gcaagtgtca acgtatagat acgtaccatc atttcctcct
    12001 ataggctgat ttcagtctgg caccgaatca gcggaaagtg aagaaattaa caatatgatt
    12061 tattagagct ttatttaaaa ttttgcagat aaatatatat aaataaaaat ctgttttcgc
    12121 cacatgctta ttcaaaaaat gaaataagaa tacattgcta agttattcgc gcctttttat
    12181 aattcgttac ctttccaggg acgctaatct ttgatcctta agaaagtttc cgggaccgcg
    12241 atggtctctt tttttgttgt acggactctt gataaatgaa gcttttgaag acagttcctg
    12301 ccatcgttat gttggcgggg ggcatgtttg cgtcactgaa tgcagctgcc gatgattccg
    12361 tttttactgt catggacgat cctgccagcg ctaaaaaacc tttcgaaggt aatctgaacg
    12421 caggatatct tgcacaatca ggcaatacga aaagctcctc acttacggct gacaccacca
    12481 tgacctggta tggacaaacc actgcctggt cgctgtgggg gaacgccagt aatacttctt
    12541 ctaatgatga gcgttcttca gaaaaatatg cggcgggcgg gcgtagccgc tttaatttaa
    12601 ctgattatga ctatttattt ggtcaggcaa gctggctgac agaccgttat aacggctatc
    12661 gagagcgcga tgtgttgacc gcgggttatg gtcgtcagtt tttgaatggc ccggtacaca
    12721 gtttccgctt tgaattcggt ccaggtgtgc gttacgacaa atataccgac aatgccagtg
    12781 aaacccagcc gctgggctat gcctccgggg cttatgcatg gcagttgact gacaacgcta
    12841 aatttacgca gggtgtttcg gtctttggcg cggaagacac aacgctgaac tccgaaagcg
    12901 cattaaatgt tgctattaat gaacattttg gactgaaggt ggcttacaac gtcacatgga
    12961 actctgaacc tccagagtct gcaccggaac ataccgaccg tcggacaacc ttatcgctgg
    13021 gctacagcat gtaaaattat ctgccggacc gtgttatgcg gtctggctgt cttcgtttgt
    13081 cactgctcag ccccaaatca ttgctgccca acgtagcaac aacatagcgc cacagattgc
    13141 cagtagcacc accaccatct tccagtgttt tttatcaaag cgtttagtcg gcataccagt
    13201 gtcctgcata tctcttgtgc gtgataacgg agtgtaacaa aagggacagg catcaaggga
    13261 ttttttgtta ttacagccgt cgggtggcgt tgtgcacatg gtcactgttt ctgcgtttta
    13321 tgaaaaaaat aaaattatgt atcgatatat atcgtaaata gctggttata aatacgtctt
    13381 tatttcatca gcactaaaac tcaggctttg gctgatgaca tttatagcag cataatatgt
    13441 ttctacagtt ttataatgct tatgatgaat ggctatatga acagctgaca tgttgtcatg
    13501 atttttagcg gccaatagtg taaataactg atgttgacta aaagaatgtt tttttgcaaa
    13561 agcacccagc gtcgatatat aagataacac gacgtcttca ttacccttgc tcatggcgat
    13621 gtataatgca ggggttccct gtgctgtagc tccttttaat aaatccatga tgttagcttt
    13681 gctcaacttg tatttaaagg cgatgccatt aattttagag aggaaccgtg tgacacacaa
    13741 agggtgatta ttttccattg cggcgtataa tcctggctca ccatcatgta ttttcatggc
    13801 taaaatactc atcaccagtt ttttgtttaa aagctcttgt gctattggag tggttaatgc
    13861 attcagaaag atatcgatta tt
//
LOCUS       DBKEKI010000096        13857 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.096, whole genome shotgun sequence.
ACCESSION   DBKEKI010000096 DBKEKI010000000
VERSION     DBKEKI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.096"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..132)
                     /gene="yjdI"
                     /locus_tag="ACUQPX_002900"
     CDS             complement(<1..132)
                     /gene="yjdI"
                     /locus_tag="ACUQPX_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HHV9057552.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     "
     gene            313..1944
                     /locus_tag="ACUQPX_002901"
     CDS             313..1944
                     /locus_tag="ACUQPX_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHV9057553.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKHNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1941..2660
                     /gene="dcuR"
                     /locus_tag="ACUQPX_002902"
     CDS             1941..2660
                     /gene="dcuR"
                     /locus_tag="ACUQPX_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HHV9057554.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3231..4571
                     /gene="dcuB"
                     /locus_tag="ACUQPX_002903"
     CDS             3231..4571
                     /gene="dcuB"
                     /locus_tag="ACUQPX_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HHV9057555.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4649..6295
                     /gene="fumB"
                     /locus_tag="ACUQPX_002904"
     CDS             4649..6295
                     /gene="fumB"
                     /locus_tag="ACUQPX_002904"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HHV9057556.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLEELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6418..7047
                     /locus_tag="ACUQPX_002905"
     CDS             6418..7047
                     /locus_tag="ACUQPX_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HHV9057557.1"
                     /translation="MIRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7186..8607)
                     /gene="melB"
                     /locus_tag="ACUQPX_002906"
     CDS             complement(7186..8607)
                     /gene="melB"
                     /locus_tag="ACUQPX_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HHV9057558.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKLWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8710..10065)
                     /gene="melA"
                     /locus_tag="ACUQPX_002907"
     CDS             complement(8710..10065)
                     /gene="melA"
                     /locus_tag="ACUQPX_002907"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HHV9057559.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            10348..11256
                     /gene="melR"
                     /locus_tag="ACUQPX_002908"
     CDS             10348..11256
                     /gene="melR"
                     /locus_tag="ACUQPX_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HHV9057560.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11455..13722
                     /gene="adiA"
                     /locus_tag="ACUQPX_002909"
     CDS             11455..13722
                     /gene="adiA"
                     /locus_tag="ACUQPX_002909"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HHV9057561.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDDAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3615 a         3600 c         3360 g         3282 t
ORIGIN      
        1 tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct gacatatcgc
       61 agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc cgtccagtag
      121 cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag catagccccg
      181 gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta tatagccaat
      241 catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga aatttgtcac
      301 acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg tccgatgaaa
      361 ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct attggtggtg
      421 catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc caacaaggca
      481 ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt gcagaaaaaa
      541 ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacacaa cgatctgctg
      601 tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc ccagcgtatt
      661 ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga aaatgtcgct
      721 atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta cgatgaaaat
      781 cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac ccaacagatc
      841 aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt cggactgatt
      901 ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga accctacgaa
      961 atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga aggcgtcgtt
     1021 gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga attgctgaat
     1081 taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg gtcacaggtg
     1141 gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga gattacgatt
     1201 aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt tatcatcggt
     1261 gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg actcgacggt
     1321 ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa taaattgcat
     1381 gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta cattctcaaa
     1441 acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa atctccggtt
     1501 atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca tacgctgatt
     1561 ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc gacgctgatt
     1621 accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga acccggtggc
     1681 gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt taacgatgat
     1741 ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt ctcgacaaaa
     1801 ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa tctcggcggc
     1861 agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca gataccctgg
     1921 gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac gcaatggtcg
     1981 cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt ggaacagcct
     2041 cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc gacctgatat
     2101 tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc ctgcataacg
     2161 cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca accattaaag
     2221 attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct tcccgctttg
     2281 aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat cagtattatg
     2341 accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag gatcctcgtc
     2401 gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg attgacgcgc
     2461 atcaggacta tgagttttca accgacgagc tggctaacga ggttaacatc tcgcgtgttt
     2521 cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc agtatccatt
     2581 atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac tactcactgc
     2641 tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt attaccgtaa
     2701 tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc cagatcaaat
     2761 ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg gtgtttttaa
     2821 tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg tgtattttta
     2881 cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc tgagaacaaa
     2941 aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat ctgcgcagta
     3001 caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt atcacagtgc
     3061 ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc taaagccggt
     3121 aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac agaacgtgac
     3181 tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac atgttattta
     3241 ctatccaact tatcataata ctgatatgtc tgttttatgg tgccagaaag ggtggtatcg
     3301 cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc caccttcagc
     3361 caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg gcatcggcga
     3421 ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag ctgctgcgcc
     3481 gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg accattcttt
     3541 gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc gccattaaga
     3601 acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag atggggatta
     3661 tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt aatgtcacct
     3721 ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca tcgacgttaa
     3781 tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg gataaagacg
     3841 aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac ggtgataccg
     3901 cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg attttcctcg
     3961 gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca tccttcggcg
     4021 gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc ggggcgctga
     4081 ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc ttccgttccg
     4141 gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc atgttcggtg
     4201 cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat ccgtgggcgt
     4261 atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg gcgctggcgg
     4321 cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg gcttcagcac
     4381 cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca gcgattcagt
     4441 ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt tttattctgc
     4501 cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc gcgatgtacg
     4561 ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca ttttcgaata
     4621 acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct accaagcacc
     4681 tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt acgttagcgt
     4741 tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagccctga ccctgctggc
     4801 gcagcaagcc tttcacgacg cttctttcat gctccgtccg gcacaccaga aacaggttgc
     4861 ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac tgcaattctt
     4921 aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata ccggcaccgc
     4981 gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg aagaagcgct
     5041 gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc aaaacgcggc
     5101 gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa tcgacctgta
     5161 cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg gctctgccaa
     5221 caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac tgaaaaactt
     5281 cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc atatcgcgtt
     5341 tgtgattggc ggtacgtctg cggaaaccaa cctgaaaacc gtcaaattag caagcgctca
     5401 ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc gcgatgtcca
     5461 gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc agtttggcgg
     5521 taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg catcctgccc
     5581 ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa tcaaccgcga
     5641 aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac aagaactgcg
     5701 tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga aagagatcct
     5761 cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca ccattattgt
     5821 gggtcgagat attgcacacg ccaagctgga agagctgatt gacgccggta aagaacttcc
     5881 gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc ctgccggtta
     5941 tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg tggatctgct
     6001 gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc agcaggttac
     6061 cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc cggcggcggt
     6121 actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc tgggtatgga
     6181 agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg atgacaaagg
     6241 taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta agtaacctct
     6301 tcggcccagc gcctggcagc atgctgccag gtgatccccc tgggccacct cttttgcgat
     6361 tgtaatttca tgcttgctgg tgaatagtca gtattttccc tgacttgcga actcaccatg
     6421 atccgcacac tcaagccgtt aattcttaac accggcgcac tggcgctaac gttaatcctg
     6481 atttataccg gcatttcggc ccatgacaaa ctcacctggc tgctggaagt gacaccggtg
     6541 attatcgtcg tgccgctact acttgccacc gccagacgtt acccgttaac gctgctgctc
     6601 tatacgctca tcttctttca cgccatcatc ctgatggtag gcgggcagta cacctacgcg
     6661 aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa tccgtatgac
     6721 aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga aattctcgtt
     6781 cgcgggatgt acgtgcgcgg acgtaaaatg gtagcgtttc tggtctgttg cgtggcgctg
     6841 gcgataagcg cgatgtatga attaatcgag tggtgggcgg cactggcgat gggccaggga
     6901 gcggatgatt ttctcggtac ccagggcgac cagtgggaca cgcaatccga tatgttctgc
     6961 gcgctgcttg gcgcattaac gacagtgata ttgctcgctc gctttcattg ccgccagcta
     7021 cggcgctatg gcttgataac cggataacaa cgtgcacttt gttgatgccg gatgcggcgt
     7081 gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa gacgtggcct
     7141 gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg ctttcacatc
     7201 actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt tgcgatattt
     7261 atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat agaaacggaa
     7321 gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa taaactgcat
     7381 acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca tccctaacac
     7441 aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag tctgcacgga
     7501 gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa cggtatctgc
     7561 caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca aaatacccgc
     7621 aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga gaacaccaca
     7681 gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata atgatttaac
     7741 taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg acagataata
     7801 ggggaacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa atccggtaat
     7861 aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa gctgatcgtt
     7921 tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag cagacgattg
     7981 attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg aaacaataaa
     8041 aaaggcgatc aataccagag tgaacatctg aaagccaaat ccccgatcgc caccgccgac
     8101 ataattaaca aatggtagcg tcacacctgc tgtaacaaag cctgccagac tggcaaaaaa
     8161 acgcggataa ggaaccagtt gttcgcgttc acgtttatcg agcgtgatgg ttggaaccag
     8221 cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt aggtcacgca
     8281 aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga gaaataagat
     8341 tacagagttt gccaacgtac cgatcaggat ccagagtttg aacttacccc atcgcgatcg
     8401 cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga tcctcgccac
     8461 cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt agtaatacat
     8521 gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa atccataact
     8581 gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca acgcccgcct
     8641 tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc agtagcgttt
     8701 agtcgcgttt taacggtgca accagcctgg cagccagtcg ccgtgggcgg caatcaggtc
     8761 gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg ggtccatcat
     8821 cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca gcagcgtctg
     8881 tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac cgactttggt
     8941 cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc cttgtggcag
     9001 gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct cgccagtcca
     9061 gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa tccgggaggc
     9121 gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc agcgtttcgg
     9181 gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgaccta gcttaataaa
     9241 ccacggcgta tattcggcaa aatgttctga cgattccgtg acgaagtagc ccagcttttt
     9301 gaacatttcg tagcgcacaa tattctggca gcgagtatta ccatgaatat ttggcttcgg
     9361 tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacgt aactgccgtc
     9421 ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg cgcaacggta
     9481 acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg ttccctgcac
     9541 cgaatggcac agcccgacct gtttgatata cggatagcgg gcatacatcg cccaggtatt
     9601 catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt ccgtcatgtt
     9661 ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac cgcccggccc
     9721 caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga aatcagtcac
     9781 cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg catcctctaa
     9841 ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg aatccatcag
     9901 cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca tcagggcaat
     9961 atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt taacgaaaat
    10021 cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga tctcctggct
    10081 tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct tccaggttga
    10141 gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt atgggaataa
    10201 attacctgaa aagtagagtt ttcggcctca tgatgctcgg acagtcccct cgcccctctg
    10261 ggaagagggt gaaaaccgtg atcccactcg cagtcatcct ccctcactcc tgccataatt
    10321 ctgatattct aggaaagaga gccatccatg aatacagata cgtttatgtg cagcagcgac
    10381 gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat ggagattgag
    10441 tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga agtgaatgtg
    10501 cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat caatcagggt
    10561 catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg aacctgtcag
    10621 agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct ggataaagac
    10681 ctgattaacc acgtcactca cggcatggtg atcaaatcac ttgccacaca gcaacttagc
    10741 ccgtttgaag tacgccgctg gcagcaggaa ttaaacagtc cgaatgagca aattcgccag
    10801 ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctag ctgggaaccg
    10861 attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca tgcgcaattt
    10921 tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct gaccatcaac
    10981 gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt ccagcgggtc
    11041 atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt tcgcgcgtta
    11101 ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt tcgttcaagt
    11161 agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca ataccgcaaa
    11221 cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta tcagccaaaa
    11281 aaatagttta gccgtcgata ataagcagtg aaaataattt aaaataatca cataacgtat
    11341 tgtttataaa actaaatatt tcaaaaatgt ttgtttttca cgcgctttac agcccgaaaa
    11401 ggccggaaga tacttgcccg caacgaagat tccttcataa ccgggtaagc aatgatgaaa
    11461 gtattaattg ttgaaagcga gtttctccat caagacacct gggtcggtaa cgccgttgag
    11521 cgtctggcag atgctttaag ccagcaaaat gttaccgtga ttaaatccac ctcctttgat
    11581 gatggttttg ccattctctc ttcaaacgaa gccattgact gcctgatgtt cagctatcaa
    11641 atggaacatc cggacgaaca tcaaaacgtc agacaattga tcggtaagct tcatgagcgc
    11701 caacaaaacg tgccggtctt cctgttgggc gatcgggaaa aagccctcgc cgcaatggac
    11761 cgggatctgc tggagcttgt cgatgaattc gcctggattc tggaagatac cgccgacttt
    11821 atcgccggac gcgccgttgc cgcgatgacc cgctaccgcc agcagctgtt gccgccactg
    11881 ttcagcgcgc tgatgaaata tagtgacatc catgaatatt cctgggcagc gccaggccac
    11941 cagggcggcg ttggttttac caaaacaccc gccggacgtt tctaccatga ctactatggt
    12001 gaaaatctgt tccgcaccga catgggcatc gaacgaactt ccctcggttc tttgcttgac
    12061 catactggcg catttggcga aagcgaaaaa tatgccgcac gcgtatttgg tgccgatcgc
    12121 tcctggtcgg tagtcgtcgg tacttccggc tctaaccgca ccatcatgca ggcttgcatg
    12181 accgataacg atgtcgtggt cgttgaccgt aactgccata aatccatcga acaaggtttg
    12241 atgctgacag gcgcgaaacc ggtctatatg gtgccaagcc gcaaccgcta cggcattatc
    12301 gggccaatct atccgcagga aatgcaacct gaaaccttgc aaaagaaaat cagtgaaagc
    12361 ccgctgacca aagacaaagc cgggcaaaaa ccgtcttact gcgtggtaac caactgcacc
    12421 tatgacggcg tgtgttataa cgctaaagaa gcgcaggatc tgctggaaaa aacctccgat
    12481 cgtctgcact ttgacgaagc ctggtacggc tatgcacgtt tcaacccgat ctatgccgat
    12541 cactatgcca tgcgcggcga acctggcgat cacaacggtc ctaccgtttt cgccacccac
    12601 tccatccaca aactgctgaa tgcgctgtca caggcttctt atattcatgt acgtgaaggt
    12661 cgtggggcga ttaacttctc ccgcttcaac caggcctaca tgatgcatgc caccacctcc
    12721 ccgctgtatg ccatctgcgc atccaacgac gtggcggtgt cgatgatgga cggcaacagc
    12781 ggcctgtcac tgacacagga agtgattgac gacgcggttg atttccgtca ggcgatggcg
    12841 cggctatata aagagttcac cgctgacggt agctggttct tcaaaccgtg gaacaaagaa
    12901 gtcgtcaccg acccacaaac cggcaaaacc tatgactttg ctgacgcacc aaccaaactg
    12961 ctgaccaccg ttcaggactg ctgggtaatg catccgggcg aaagctggca cggcttcaaa
    13021 gatattccgg ataactggag tatgctcgac ccaattaaag tcagcatcct tgctccggga
    13081 atgggtgaag atggtgaact ggaagaaacc ggtgttccgg cggcgctggt cactgcctgg
    13141 cttggtcgcc acggcattgt gcctacccgc accactgact tccaaattat gttcctgttc
    13201 tctatgggcg taacccgtgg gaaatggggg actctggtta acaccctttg ctccttcaaa
    13261 cgccactatg acgccaacac accgctggcg caggtgatgc cggaacttgt tgaacaatat
    13321 cctgacactt acgcgaacat ggggattcac gatctgggtg acaccatgtt tgcctggctg
    13381 aaagaaaaca accctggcgc acggttgaac gaagcctatt ccggcctgcc ggtggcggaa
    13441 atcaccccgc gtgaagcgta caacgcgatt gtcgacaaca atgtcgaact ggtatccatt
    13501 gaaaatctgc caggacgcat cgcggcaaac tcagttatcc cgtatccgcc aggaatcccg
    13561 atgctgctgt ctggtgaaaa cttcggcgat aaaaacagtc cgcaagtaag ttatttacgc
    13621 tcgctgcaat cctgggacca ccatttccct ggatttgaac acgaaactga agggactgaa
    13681 attattgacg gtatttacca cgttatgtgc gtgaaagcgt aaccactatt ccgctgaagg
    13741 cgtaattgtt taaataacat tacgccgcct ggccttaggc cttttgagta tggcaacgtt
    13801 ttcataaaaa ttgctgcaaa caaaaatgtc atactttttg cgcggcccca ccccgcg
//
LOCUS       DBKEKI010000097        13722 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.097, whole genome shotgun sequence.
ACCESSION   DBKEKI010000097 DBKEKI010000000
VERSION     DBKEKI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.097"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..419
                     /locus_tag="ACUQPX_002910"
     CDS             <1..419
                     /locus_tag="ACUQPX_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HHV9057562.1"
                     /translation="CLSWTVIHYRDNAITYKEQRDKNARELKLANATITDMQQRLRDV
                     NALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVE
                     LSPVAGRNVLGIRDGIISDQTALRMLQEYIRAQCLK"
     gene            622..1218
                     /locus_tag="ACUQPX_002911"
     CDS             622..1218
                     /locus_tag="ACUQPX_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HHV9057563.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPRVLGDAAAKFFATDTYINGTGGVVERDICNFPKREACLMAMSYS
                     YELQAQVYDHMTELEGGKDINLLDFSGLTDMAISEMQNRVAAAEKFSFEMHGQAGSAL
                     MTRRKKEKKAIKKAEQLVKDLIQFKLCDMGDFPDGKPA"
     gene            1551..2105
                     /locus_tag="ACUQPX_002912"
     CDS             1551..2105
                     /locus_tag="ACUQPX_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HHV9057564.1"
                     /translation="MAKLTDKQELFAREYLKDLNATQAAIRAGYSEDSAAVQGCKNLI
                     KANIAKRIAELKAERNEEVGIDAAYVLRRLVEIDQMDVLDILLQNGELKPIKDWPKVW
                     RTTLSGMDVVEMVSADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNEVTGADG
                     GPVRTEITKLTPEQAAEVYRKMMG"
     gene            2108..3727
                     /locus_tag="ACUQPX_002913"
                     /pseudo
     CDS             2108..3727
                     /locus_tag="ACUQPX_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130781.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
     gene            3727..5268
                     /locus_tag="ACUQPX_002914"
     CDS             3727..5268
                     /locus_tag="ACUQPX_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HHV9057565.1"
                     /translation="MWPFKRKEPSAPAVPDKQPEPQPPEINDEVIASVRQKPRREFVR
                     YEPPPGVIPAPVRDAVLAMDATPYDTLNSHYPDFVYGGFPGYPYLALQAQLPEYRRMV
                     SVLAEEMTRKWIKVKAVGEGDDSHASRIAQLTDALEHYNVQDVFRLAIEHDGFFGRGQ
                     IYIDVRSPSGISAWTDPAELGSGLFISDKKIPKGSLLGLRVIEPVWTYPGIYNADNPL
                     SDDFYRPSEWYVMGKTVHASRMIDLISRPVPDMLKPAYNFGGLLLVQIAEPYVDNWLR
                     TRDSVGDMLHSFSLSGIKTDMTQVLSGKRDPNYAKRAELFNRTRDNRGLLMLDKTNEE
                     FFQFNTPLSGLDTLQAQAQEHMFFVSAIPSVKFAGLSPTGLNASSEGEIRVFYDTIAA
                     LATRLLKKPLKKVLDIIQLSEFGDIDPDITFEFEPLHELTREQLANIRKTEAETDQIY
                     ESTGAVTNNEVRERLATAQDSPYSGIDLSGDIEIDDTEIDNTEENPPQGPNADPEADF
                     TQRGD"
     gene            5309..5998
                     /locus_tag="ACUQPX_002915"
     CDS             5309..5998
                     /locus_tag="ACUQPX_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000552018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HHV9057566.1"
                     /translation="MHNSVLYWLRAEYRKTDLAQDASPVNLMRGAMQQLARRWQKNFD
                     EMALRLARRFAGDVLKNSDASLYTALRDAGFTVPFRMTTEMNTALQASITENVNLIRS
                     IPQQHLPQVETLVMQSVGRGRDLKTLTDELEKRYGITRRRAALIARDQNNKATSVMQA
                     ARQRSVGITEGVWRHSRAGKTWRPSHVKANGKRFDLRKGMFLDGKWVLPGEEINCRCG
                     WEAVIPGLEKK"
     gene            5995..7344
                     /locus_tag="ACUQPX_002916"
     CDS             5995..7344
                     /locus_tag="ACUQPX_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HHV9057567.1"
                     /translation="MIITEMLAFDRASVRQFDKVGRLQIERSNLSKANVCGYFGYEIP
                     GAEALGLDPKKLYQLYRDPDELRKAVSTFNNIPVLCRHKPDYPGAPAREYRVGTTHAN
                     SEFDGTYLVNGMSIWDNSAIAGIETDEQREISSSYAYVADMTPGTTPDGELYDGVMRN
                     IVGNHVALVGDGRVGSDCLVMDSLPQELKRMKLSKKEVAVLTALGTYLAPRLAQDAAP
                     GDLLRLMAQYKRPAAIASAVKTAYSERLVQDMDIEPVELAQLMESAEAVPELVGDDDT
                     GLTDESKAFDTDSPMESVLALLSGKVPDDVLEKIKSALSPTTDEAPETKEADVKPDDV
                     KVDKPAMDAAIRLATDQATKRAAENFRAVRVAEAEVRPLIGDVVAMDCAEDVYRTALE
                     QTGIDIQGIHPGAYRSMVKFAVEQKQTAKSSPRVAMDQASASTFAADFPGAKLKRGY"
     gene            7346..7828
                     /locus_tag="ACUQPX_002917"
     CDS             7346..7828
                     /locus_tag="ACUQPX_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="structural cement protein Gp24"
                     /protein_id="HHV9057568.1"
                     /translation="MNTFQTHMNQYPAPGIPGAFASANPHASYVAGDGGLITGPDGLV
                     IARFAWVTKGVAANKGAGAPAGFVPRDGQASIVEWLAGDSNTIYPGRECTLMVSGDFW
                     ALTTTTATIGQKVFASLATGEIATGAAGTSMAGFVETWFSVASAAAAKEVIKISTWSK
                     "
     gene            7828..8856
                     /locus_tag="ACUQPX_002918"
     CDS             7828..8856
                     /locus_tag="ACUQPX_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HHV9057569.1"
                     /translation="MNKFKQHYATASRDYGIILPGAQAYLPKEYAADYGLAMDAQPAL
                     VTAANSGIPAYFTNYVEPELIRVLVTPMKAAQILGETKKGDWTTLSAQFPIAESDGEV
                     SSYGDYSNNGVVSANVNWVPRQSYHFQTFTVWGERELEMYGAARIGWAAELNVASALT
                     LNKFQNKSYFYGIAGLTNYGLLNDPSLSAPITPDTVDGKLKWDDKDGQGVYDDIVKLF
                     KQLVKQTNGHIERTDKMKLCMSPLAEVNLTKTNQYRVNVSDLLAKNFPAMTIETAVEY
                     TSDAGELVQLIAERLGEQDTGYCAFTEKMRAHAVVADTSSWKQKKSAGTWGAIIRQPL
                     AYAQMLGV"
     gene            8860..9207
                     /locus_tag="ACUQPX_002919"
     CDS             8860..9207
                     /locus_tag="ACUQPX_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057570.1"
                     /translation="MAEMVTVGCKLPNGLVLEVGQKRVQVAGWRNNAVKIVGGYGLTQ
                     VDKVFFDAWLAEHGQQPYVKNGVIFAQDKANSAVSQATEQKAVKSGLEPLPQKNPAPG
                     VNRNDEVMGKPQE"
     gene            9213..9671
                     /locus_tag="ACUQPX_002920"
     CDS             9213..9671
                     /locus_tag="ACUQPX_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HHV9057571.1"
                     /translation="MGTVTFDCRMFVSLYPEFSMVTPELLTAVFNQATALYLDNTDDS
                     PVTDLKEREQLLFLLVAHLCALRGLGNGRDGQAGLVGRITSASEGSVSVSVDNSGSND
                     ASWWYLQTPWGADYWQATAPYRSMQYHSGGAPSRYPGHYYRGYGRGVDGK"
     gene            9661..10245
                     /locus_tag="ACUQPX_002921"
     CDS             9661..10245
                     /locus_tag="ACUQPX_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057572.1"
                     /translation="MVNKVTGGKQFRQKLKQVAANLSLGKKLKVGFLEGATYPDGTSV
                     AYIAAVQEFGGRAVIPAREQTLHFRYNEKTGETGHRFVKAGKGNFVQDVVIPEHTVTI
                     PPRPFFRKMIEHKSPEWGEKMATLLRANDFDTATALVCMGEHIKGQLQMSVRDWESPP
                     NAASTARQKGFNNPLIETGHMMDSVDYSVDGGKK"
     gene            10242..10607
                     /locus_tag="ACUQPX_002922"
     CDS             10242..10607
                     /locus_tag="ACUQPX_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057573.1"
                     /translation="MNFHGIVSGAVRRVNPFTDALVYRSRGSTQQADYSRVPEYEDPV
                     SVRVQKQAVTQADLRHLDNLNQQGVFATLYTDGNWCGLNRARQQGGDKFVIGDETWLV
                     IEVPEIWPDWTRVIVCLQV"
     gene            10592..11137
                     /locus_tag="ACUQPX_002923"
     CDS             10592..11137
                     /locus_tag="ACUQPX_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage neck terminator protein"
                     /protein_id="HHV9057574.1"
                     /translation="MPSGVTLSVTESALYQALGDYLRGLFSDAGIERTQQNQVPMPQG
                     NFITMTGIDVTGLSTAVVAYSAPAQAGEGSQHITRTTKWRCQLDFYGPHAADNAQVLA
                     TLFRSEFSVRLFRQGGGLISPLYCSEPFNTTFPNGQQQYEPRWTLDIQMQINPVVTTS
                     LMFFDNVITRTTEADNANSTQ"
     gene            11118..12602
                     /locus_tag="ACUQPX_002924"
     CDS             11118..12602
                     /locus_tag="ACUQPX_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HHV9057575.1"
                     /translation="MPIPLSKDVQINPGVLAVAGNAVDLNGLLLTSNPLLPVGTVVPF
                     SSPDDVAAYFGALSDEYARAQLYFQGFKNATRTPGQLLFSRFSPAPSAAWLRSGSFKG
                     ATVEQLQKLSGTLMLSINGKSVSIEVNFNGVASFAAAATALQTALTAAVATVVFDTTQ
                     NAFVITVAGAKPGSTTITFGSGSAAELLKMTSNTGAVISRGAPVSDVPDTMAAIKAAS
                     QQWAGFSTVSEVTDEQHLAFSAWANGQGKRYFYVAWTTSGNARVKGSTEHIAYQMIAV
                     NNYSSVVPVFATDGNRAAAVLGYAACLDFVRPEGRVSFKFREYEGLAADVTSGSDYDA
                     LIAAGYNFYGKYAENSVVEDYWADGTITGDFKWLDSFCGQIWLNASLQGAVIALFKSN
                     QTIPYNNEGRALVAASMSDVIQQYKRWGGIREGVTLTEAQKKQINNVVGADVSSTLFA
                     TGYYLYIGDMLPSLRAPRSSPSCTLWYCDGGSIQKLVIASTEVQ"
     gene            12603..13049
                     /locus_tag="ACUQPX_002925"
     CDS             12603..13049
                     /locus_tag="ACUQPX_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber protein"
                     /protein_id="HHV9057576.1"
                     /translation="MSGNNNTITAADAIITLTVNNLYPSGVRLQGFATDNVYGTDPLV
                     LAETARGIDGKLSAGFVYSNIIQTFHIMPDSPSRDIFDTWSTTSRASRAVFRCNAVVL
                     LPAIGRKYTCVNGVLKQWKALPDAARTLQPGQAVIEWETITPEVFN"
     gene            13049..13453
                     /locus_tag="ACUQPX_002926"
     CDS             13049..13453
                     /locus_tag="ACUQPX_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057577.1"
                     /translation="MARKEKFITIEGQGRDKGKVFHLTEMSASQAEWWAMRAIMAMGR
                     GGVELPDDVRSLGMAALALEGLKALSKIPPDEARPLMDEMMDCVQFVPDPKKRSIRRP
                     LVEEDIEEIITRFNLRAEVFKLHVDFFATVVS"
     gene            13495..13677
                     /locus_tag="ACUQPX_002927"
     CDS             13495..13677
                     /locus_tag="ACUQPX_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HHV9057578.1"
                     /translation="MVDYVNVPRTIATVISSGKASKAELDSVLGVQDLWDLLEIIHVD
                     AHNEQVIQENRNGAGT"
BASE COUNT         3364 a         3291 c         3932 g         3135 t
ORIGIN      
        1 tctgcctgtc atggactgtt attcattacc gtgataacgc cattacctac aaagagcagc
       61 gcgacaaaaa cgcaagagaa ctgaagctgg cgaacgcaac cattactgac atgcagcaga
      121 gactgcgtga tgttaatgca ctcgatgcta aatacacgaa ggagttagct gatgcgaaag
      181 ctgaaaatga tgctcttcgg cgcaagcttg ataatggtgg tcgggtgctc gtcaaaggaa
      241 aatgccctgt gccatcctca gccgaaacct ccagcgcctc cggcatgggc aatgatgcca
      301 ccgtcgaact ctctccagtt gctggacgaa acgttctcgg tatccgggac ggaatcatca
      361 gcgaccagac agcattgaga atgcttcagg agtacatcag ggctcagtgc ctgaaataaa
      421 tttttttgca aatcacaaag tccatttaat gagcctcgca cttgcggggc ttttatatgt
      481 ccgcagtaaa ccgcgcatcg cagcgcgtaa caatcccgag tttttcagaa agctgagcct
      541 gagaactgcc gtataggtga ggacctctcg gggacggctt ttctgtgcga acaggctcaa
      601 ctttctaaag gaaataccga catgaacaaa tcattaaccg ttcttccttc tggcgaatac
      661 ccgaccatga gcagtcttga gatggtggat tacattaatg ctgatcggaa atccaaagcg
      721 gaggcggagg ggcttgcgtt cccctgtaag aaatatcgca aactcgaaca ccgcagtttc
      781 atgaagaaag tacccagggt cctcggggat gcagctgcaa aattttttgc aactgatacc
      841 tatatcaacg gaacaggtgg tgttgtggag agggatattt gcaattttcc caagcgtgaa
      901 gcttgcctca tggcaatgag ctacagctat gagcttcagg cgcaggtgta tgaccacatg
      961 acggagcttg agggcgggaa ggacattaac ctcctcgatt tctctggcct gaccgatatg
     1021 gcaatcagcg aaatgcaaaa ccgtgtcgcg gctgctgaga agttctcatt tgaaatgcac
     1081 ggtcaagcag gtagcgctct catgactcgt cggaagaaag agaagaaggc cattaaaaag
     1141 gctgagcagc ttgtgaagga tctgattcag ttcaagctat gtgacatggg ggatttccct
     1201 gatggtaaac cagcatgact ccgattgatt tcatacataa aaatgtaaca accgagttaa
     1261 taaagcttgg gtacgaccag aacgcggcta tgactggagc tgacatggcg gtggagcatt
     1321 accgccgttg ttcacaagcc agcagaaaag gccgagtttt cgatgactgc ctgtacattg
     1381 caaaacagtg ggcaggtagg cagaaaaggg aaaaaatgac aacctgagag ctactttcac
     1441 aacggctctt cattacaaag cctatctacg ggtgggcttg ataatggctt ataccctaca
     1501 cgggataact taactgatat cccttttaac ggataaacgg agcacaaata atggcaaagc
     1561 tcaccgacaa acaagagctg tttgcccgtg agtacctgaa agatctcaat gccacgcaag
     1621 cagccatcag ggcgggttac agtgaggatt ctgccgccgt acaaggatgc aaaaacctaa
     1681 taaaagctaa tatcgcaaag cgcattgctg agcttaaagc cgagcgaaac gaagaggtag
     1741 gtatagatgc cgcttatgtt cttcgtagat tggttgaaat cgaccagatg gatgtgctcg
     1801 acattctcct gcaaaacggt gagctaaagc ccattaaaga ctggcctaag gtatggcgca
     1861 caacgctatc aggaatggat gtcgtggaga tggtatccgc agatagcgcc gcacttctga
     1921 agaaaatcaa atggcctgat aaggttaaaa accttgagtt gcttgggcgc catgtttctg
     1981 ttcaggcgtt taaagacaac gtcaaaaatg aagtgactgg cgctgacgga ggaccagtca
     2041 gaacagaaat taccaaatta acgcctgagc aggccgcaga ggtgtataga aaaatgatgg
     2101 gctaagtatg ccgttaccat tccccttcga ttttaaacat cctgattacc agatggtttt
     2161 tgaatggcgg atggaacgcc tacagcgcat tcgccagaac cctgaaatat tgcccgtatt
     2221 gaagcagttt taccgaacca atccggctca gttcatcatc gactggggca tgacaacgga
     2281 cccgcgtaat attgattatg gcctgccggt gaccattccg tttttactct tccctaagca
     2341 ggaggagtgg atccactgga ttatggaacg ctggagcaat cgggagaatg gtattaccga
     2401 aaaatcccgt gaaatggggc tcagttggac cgcgatcgga ctggcctgct cgctttgtct
     2461 cttcaacaaa gaaatggtta tcggtttcgg ctcccgtaaa gaggaatacg tcgacagcac
     2521 cggtgacccg aaagcattgt tctggaaggc gcgcaagttc gtggaaactc tacctgtaga
     2581 gtttcgcggt tcgtggagcg agaagaagca cgcgccatat atgcgtgttg agtttcctga
     2641 aactggcgcg gttatcaaag gtgaggctgg cgataatatc ggtcgtggtg accgtacgac
     2701 cctttatttt gtggatgagg ctgcttttct ccagcgacca ttacttattg atgccgcgct
     2761 ttcccagaca acccgatgtc gtatcgatct ctcatcggtt aacggcatga acaacccctt
     2821 cgcacagaag cggcacagtg gaaaaatccc ggtgtttacg tttcactggc gtagcgatcc
     2881 gcgtaaagac gatgagtggt atcacaagga gtgcgagaaa attgataacc cagtcatcgt
     2941 tgcctaggaa ctggatctta attaccaggc atcagcagaa ggcatcctga tcccctcaga
     3001 atgggtacag gctgcggttg atgcacatat cagactgggc attcagcccg gcggccaacg
     3061 gcttggtgca atggatgtcg ccgacgaagg gcgggataaa aacgcctgtt cgctccgtta
     3121 tggcatcctg ctgaatgacg tacaggagtg gtcaggtaag gggagtgaca tctatgactc
     3181 tgtggttaag gtttttggtc tgtgcgatga ctttggtgcc gatgagtttc gctttgatga
     3241 gggcggattg ggtgctggcg ttcgtggtga tgcgcgcgct atcaacgaac tgcgtgaagc
     3301 ggagggcatc tgtcaaatca ccgccactcc attccggggc agcgggagcg tgtttcatcc
     3361 tgaaaatgaa gctgttcccg gtgataatgg taaaccggct cgtctgaata aggacttttt
     3421 tgtcaatgcc aaagcccagg ggtggtggca tcttcgcaaa ttattccgca atacatttcg
     3481 tgcactacag ggtatggagt atgacccgga tgagattatt tccatcagca gcacaatgga
     3541 aaataaagac cggttgttga tggagctgtc acaacctacc tggtcaaaaa atgccaccgg
     3601 aaaaattctt gtggataagc aacctgacgg aacgaaatct ccaaacctgg ctgattctgt
     3661 gatgattgct tatgcaccga tggaaatgcc catcgtaatt tctgatgact ttatggagtg
     3721 gatctgatgt ggccgtttaa acgaaaagaa ccgtcagcgc cggcggtacc ggacaaacaa
     3781 cctgaaccac agccaccgga aattaacgat gaggtgatcg cttccgtccg gcagaaacca
     3841 cggagggagt ttgttcgcta tgagccacca ccgggcgtta tcccggcacc ggttcgcgat
     3901 gctgtgctgg caatggatgc aacaccgtat gacaccctga acagtcatta tcctgatttt
     3961 gtttatggtg gtttcccggg ctatccgtat ctggcacttc aggcgcagtt accggagtac
     4021 cggcgtatgg tcagtgtgct tgccgaggag atgacccgta agtggataaa ggttaaggcg
     4081 gtcggagaag gggacgacag ccacgcgtca cgcatagcgc agcttactga cgcactggag
     4141 cactataacg tacaggacgt tttcagactg gcgattgagc acgacggctt tttcgggcga
     4201 gggcagattt atattgatgt gcgttcgcca tcgggtatat ctgcctggac tgacccggcg
     4261 gagctgggat ccggactgtt tatttccgac aaaaaaatcc cgaaaggttc cctgctgggg
     4321 cttcgtgtta ttgaacccgt ctggacgtat ccgggtatat ataacgcgga taatccgctg
     4381 agtgatgatt tttaccgtcc gtccgaatgg tacgtaatgg gaaaaacggt tcacgccagc
     4441 cgcatgattg atctgatctc ccgtccggtt ccggacatgc tgaagccggc ctacaacttt
     4501 ggcggcctgt tactggttca gattgctgag ccttatgttg ataactggct gcgaacacgc
     4561 gacagcgtgg gcgatatgct gcattcgttt tcgctgagcg ggattaagac ggatatgaca
     4621 caggtattaa gtggtaaaag ggacccgaat tacgcaaaac gcgcggagtt gtttaaccgt
     4681 acccgtgata accgcgggct gctgatgctg gacaagacga acgaagagtt tttccagttc
     4741 aacactcccc tgagcggcct cgacaccctt caggcgcagg cacaggaaca catgttcttt
     4801 gtcagtgcca taccgtccgt aaaattcgcc gggctgagcc caacgggact gaatgcgtcg
     4861 agtgagggtg aaatccgggt gttttacgac accattgctg cacttgccac ccgtcttctg
     4921 aagaaaccgc tgaaaaaggt gctggatatt atccagttgt ctgagttcgg cgatatcgat
     4981 cctgatatca cgtttgaatt tgaacccctg catgagctga cgcgagagca actggcgaat
     5041 atccgtaaaa ctgaggcgga aacggatcag atttatgaga gcaccggagc agtgaccaat
     5101 aacgaagtac gcgaacggct ggcgactgcg caggacagcc cgtacagcgg tattgacctg
     5161 agcggggaca tcgaaattga cgacactgaa attgacaaca ccgaagaaaa tccgccgcaa
     5221 ggcccgaatg cagaccctga ggcggatttc acccaacgcg gggattgagg cctggtaccg
     5281 cagacaactg gataatgccg tcagtgggat gcacaacagt gtactttact ggctgcgggc
     5341 tgagtaccgt aaaacagacc tcgcgcagga tgcgtccccc gttaacctga tgcgtggtgc
     5401 catgcagcaa cttgcccggc gctggcagaa aaattttgac gaaatggccc tgcggctggc
     5461 gaggcggttt gccggtgatg tcctgaaaaa cagcgatgcg tcactgtaca ctgcgctccg
     5521 tgatgccggg tttacggttc ctttccgtat gacaacggag atgaataccg cacttcaggc
     5581 cagcatcacg gagaatgtga acctcattcg ctccatcccg cagcaacatc tcccccaggt
     5641 ggaaacactg gtcatgcagt ctgttggccg gggacgtgac ctgaaaacgc tgaccgatga
     5701 actggaaaaa cgttacggca tcacacgacg gcgcgcggcg ctgattgccc gcgaccagaa
     5761 caataaggca acgtcggtaa tgcaggccgc cagacagcgt tcggtgggta tcactgaagg
     5821 tgtctggcgg cattcccgcg cgggtaaaac atggcgtccg tcgcatgtga aggcgaacgg
     5881 taaacggttt gacctgcgaa aggggatgtt tctggatggt aagtgggtac tgccgggcga
     5941 agaaatcaac tgccggtgtg gctgggaggc cgttattccc ggactggaga aaaaatgatt
     6001 attaccgaaa tgctggcgtt tgaccgggca tcggtaaggc agttcgataa agtcggtcgc
     6061 ctccagattg agcgcagtaa tctcagcaag gcgaacgtct gcggttattt cgggtatgaa
     6121 ataccggggg cggaagcgct gggactcgac cccaaaaaac tttatcagct ttaccgtgac
     6181 cccgatgaac tgcgcaaggc agtttcaacc ttcaacaata ttcccgtcct gtgccgacac
     6241 aaacccgatt atccgggcgc gcccgcgcgc gagtaccggg tggggacgac tcatgccaac
     6301 agcgagtttg acggtaccta tctggttaac ggcatgtcca tctgggacaa ctccgccatc
     6361 gcggggatag aaacggatga acaacgggaa atctcatcgt catatgccta tgtggcagat
     6421 atgacgccgg gaaccacccc cgacggtgag ctgtatgacg gcgttatgcg gaatatcgtg
     6481 ggaaatcatg tggccctggt cggcgatggc cgggtagggt cggactgtct tgttatggac
     6541 tctctccctc aggagctaaa acgcatgaaa ctgagtaaaa aagaagtggc ggtgcttacc
     6601 gcgctgggaa cctatcttgc gccgcgtctg gcgcaggatg cggctcccgg ggatttgtta
     6661 cgcctgatgg cgcagtataa gcgtccggca gctatcgcca gcgcggtaaa aactgcttac
     6721 agcgaacggc tggtacagga tatggatatt gaaccggtgg agctggcgca actgatggaa
     6781 tcagcagaag ccgtaccgga gctggtcggg gatgatgata ccgggttaac tgacgagtcg
     6841 aaggcatttg ataccgacag cccgatggaa agtgtactgg cgttgctgtc cggcaaagtt
     6901 cctgatgatg tgctggaaaa aattaaatcc gcactgtctc cgacaaccga cgaagccccc
     6961 gaaacaaaag aggctgatgt gaaacccgac gatgtgaaag tcgataaacc tgcgatggat
     7021 gcagcaatca ggctggcaac tgaccaggca acgaaacggg ctgctgaaaa tttccgcgcc
     7081 gtccgtgtgg ctgaagctga agtgcgaccg ctgattggcg atgtggtggc gatggactgc
     7141 gccgaagatg tttaccgtac cgcgctggaa cagacgggga tcgatattca gggtattcac
     7201 cccggtgcgt accgcagcat ggtgaagttt gccgttgagc agaagcagac ggccaaatcc
     7261 tctccccgtg tggcgatgga tcaggccagc gcatcgacgt ttgcggcaga tttccccggt
     7321 gcaaaactga aacgaggtta ctgatatgaa tacttttcag acacacatga accagtaccc
     7381 ggcaccgggt attccggggg cgtttgccag tgctaaccct catgcctcgt atgtggcggg
     7441 agatggcggg ctgattaccg gccctgacgg gctggtaatt gcccggtttg cctgggtaac
     7501 caaaggtgtt gccgctaata agggggccgg cgcaccggcg ggttttgttc ctcgtgacgg
     7561 gcaggcttcc attgtggaat ggctggccgg ggactcgaac accatttatc cggggcgtga
     7621 atgcaccctg atggtgtcgg gggatttctg ggcgctgacc accactactg cgacgatcgg
     7681 gcagaaagta tttgcctcac tggccaccgg ggagatagcc acgggggcgg ctggcacctc
     7741 gatggcgggg tttgtcgaaa cctggttttc cgttgccagc gctgcggcgg caaaagaagt
     7801 cattaagatc agtacctgga gcaaataatg aataaattta aacagcatta tgcgacggca
     7861 agtcgcgact acgggattat tcttcccgga gcacaggctt atttgccgaa ggagtacgct
     7921 gccgattacg gactggcgat ggacgcgcag cctgcgctgg tcacagcggc taacagcggt
     7981 atccctgcgt atttcaccaa ttacgttgag ccagaactga tccgcgtgct ggtgacgccg
     8041 atgaaagccg ctcagattct gggcgaaacc aaaaaaggtg actggacgac actgtcggca
     8101 caattcccga ttgcagaatc tgacggggag gtgagttcct acggggatta cagcaacaac
     8161 ggggtggtaa gcgcaaacgt caactgggta ccgcgccaga gctatcactt ccagaccttc
     8221 accgtatggg gcgaacgtga gctggagatg tacggcgcag cccgtattgg ctgggcggca
     8281 gagctgaacg tggcgtcagc actgacgctg aataagttcc agaataagtc ctatttctac
     8341 ggtattgccg gactgacgaa ctacggtttg ctgaatgatc cgtcgttatc tgcaccaatc
     8401 accccggata ccgttgacgg taaactgaag tgggacgaca aggacggaca gggcgtgtat
     8461 gacgatatcg tgaaactctt taagcaactg gtgaaacaga ctaacggtca tattgagcgt
     8521 accgacaaaa tgaaactgtg catgtcgccg ctggcagagg tgaacctcac caaaacaaac
     8581 cagtacaggg ttaacgtgtc cgatctgctg gcgaaaaatt tcccggcgat gaccattgaa
     8641 acggcggtgg aatacacctc tgacgctggc gagctggtac agcttatcgc ggagcgtctg
     8701 ggtgaacagg atacaggcta ttgcgctttc actgaaaaaa tgcgcgccca tgcggtagtg
     8761 gcagatacgt cttcctggaa acagaaaaaa tccgctggta cctggggggc gattattcgc
     8821 cagccgctgg cgtatgcaca aatgctgggg gtgtgagtta tggctgaaat ggtaacagtg
     8881 ggctgcaaac tgccgaacgg tctggtgctg gaagtgggtc agaaacgggt tcaggtggca
     8941 ggctggcgga ataacgcggt taaaattgtg gggggctatg gtctgacgca ggtcgataag
     9001 gtgttttttg acgcctggct ggcggagcac ggacagcaac cttatgtgaa aaatggcgtt
     9061 atttttgcgc aggataaggc gaacagtgca gtctcgcagg ctacggagca gaaagccgtt
     9121 aaatccggcc ttgaaccgct gccgcagaaa aatccggctc cgggcgttaa ccgcaatgat
     9181 gaagtgatgg gtaaacctca ggagtaaacg ctatgggtac ggtgacgttt gactgtcgga
     9241 tgtttgtcag tctgtacccg gagttttcca tggtgacacc ggagctactg acggcggtgt
     9301 ttaaccaggc gaccgcgttg tacctggata acacggacga cagtccggtt accgacctga
     9361 aggagcggga acagcttctg ttcctgctgg ttgcgcatct gtgcgcattg cgggggctgg
     9421 gaaacgggag agacgggcag gctggtctgg tggggcgtat caccagtgcg tcggaaggtt
     9481 ctgtatcggt atcggtggat aatagcggca gtaatgatgc gtcgtggtgg tatctccaga
     9541 ccccctgggg tgctgactac tggcaggcga cagcgccata ccgctcaatg cagtatcatt
     9601 ccgggggggc accttcgcga tatccggggc attattaccg gggatacggg aggggcgtcg
     9661 atggtaaata aagtcacagg tggcaaacag ttccggcaga agctgaaaca ggttgccgcg
     9721 aacctttcat tgggtaaaaa actcaaagtg ggttttcttg agggagcaac ttaccctgac
     9781 ggtacgtcag tggcttatat tgccgcagtt caggagtttg gtggaagagc ggtcataccc
     9841 gcccgtgagc agacgctcca ctttcgctat aacgaaaaaa cgggagaaac cgggcaccgc
     9901 tttgtcaaag ccggtaaagg gaattttgtt caggatgtgg ttattcctga gcacacggtc
     9961 accattcccc cccgtccgtt cttccgtaag atgatcgaac ataaaagtcc cgaatggggc
    10021 gaaaaaatgg cgaccttatt acgggcgaat gattttgata ctgcgaccgc tctggtgtgt
    10081 atgggagaac atatcaaagg gcagttgcag atgtctgttc gcgactggga aagtccgccc
    10141 aacgctgcat ccactgcccg gcaaaagggt tttaacaacc cgcttattga aaccggacat
    10201 atgatggaca gcgttgatta ttctgtagac gggggcaaaa aatgaacttt cacggtattg
    10261 tttccggcgc agtacgtcgg gtaaatccct ttacagacgc gctggtttat cgttcccgcg
    10321 ggagtacaca gcaggcagat tattcccgcg tacctgagta tgaagatccg gtttccgtca
    10381 gggtgcaaaa gcaggctgtc acccaggctg atttacgtca tcttgataat cttaaccagc
    10441 agggagtttt cgccacattg tataccgatg gtaactggtg cgggcttaac cgtgcccggc
    10501 aacagggagg cgataaattt gttattggcg atgaaacgtg gctggtgatt gaggtaccgg
    10561 aaatctggcc tgactggacg agggttattg tatgccttca ggtgtgaccc tctccgttac
    10621 ggaaagcgct ctttatcagg ccctcggtga ttatctccgg gggctttttt ctgatgccgg
    10681 gattgaacga acgcagcaga accaggtccc gatgcctcag gggaatttca tcaccatgac
    10741 gggcattgat gttaccggat tatccactgc ggtagtggca tattctgcgc cagcgcaggc
    10801 tggtgagggc tctcagcata tcacccgtac cacaaaatgg cgttgccagc ttgatttcta
    10861 cggccctcac gcggcagata acgcgcaggt gctggcaacg cttttccggt ctgaattttc
    10921 cgtacggctt ttccggcagg gcggtgggct gatttccccg ctgtattgct cagagccctt
    10981 taacaccaca ttccccaacg gtcagcaaca gtatgaaccg cgctggacgc ttgatattca
    11041 gatgcagatt aaccctgtgg tcacaacttc cctgatgttt tttgacaacg tgatcacccg
    11101 gacaacggag gctgataatg ccaattccac tcagtaaaga tgtacagata aatcccggtg
    11161 tgctggctgt ggcgggtaat gccgttgatc ttaacggcct gctgttgacc agtaatccgt
    11221 tacttccggt cggcactgtg gttccgtttt cctctccgga tgatgtggcc gcgtattttg
    11281 gtgcattatc cgatgagtac gcacgcgctc agctttattt ccagggcttc aaaaatgcca
    11341 ccaggacgcc ggggcaattg ttgttttccc gtttcagtcc tgccccatcg gcggcctggt
    11401 tacgtagcgg ttcgtttaag ggcgcgactg ttgaacagct acaaaaactt tccggcacgc
    11461 tgatgctgag tattaacggg aaaagcgtca gcattgaagt gaattttaac ggtgtcgcca
    11521 gcttcgccgc tgctgcaacg gcactacaga cagcgctgac cgcagcagtg gctacagtgg
    11581 tattcgatac cacacagaat gcttttgtca ttactgtcgc cggggcgaaa cccgggagta
    11641 ccacgataac gttcggcagt ggatcggcag cagaactcct gaagatgacc agtaacaccg
    11701 gtgcggtgat atcccggggc gcgcctgtat ctgatgtccc tgacacaatg gcagccatta
    11761 aggccgcttc ccagcaatgg gcgggttttt ccacggtatc tgaggtcact gacgagcaac
    11821 acctggcgtt ttctgcatgg gcaaacgggc agggcaagcg ttacttttat gtggcatgga
    11881 caaccagcgg caacgccaga gtaaaaggca gtactgaaca catcgcttac cagatgattg
    11941 ctgtcaataa ctacagctct gttgtacctg ttttcgcgac tgatggtaac cgggctgcag
    12001 cggtactggg gtatgcagcg tgccttgatt ttgtccgacc agaggggcgt gtatcattca
    12061 agttccgcga gtatgagggg ctggccgctg atgttaccag tggcagcgat tatgatgcgc
    12121 tgatcgccgc aggttacaat ttctacggaa aatatgcgga aaacagtgtg gtggaagatt
    12181 actgggcgga tggcactatt accggtgatt ttaaatggct ggacagtttc tgcgggcaaa
    12241 tctggctgaa tgccagtttg cagggggcgg tgatcgcgtt attcaagtca aaccagacga
    12301 tcccctacaa caatgaaggg cgggcgctgg ttgcagcgtc aatgagtgac gttatccagc
    12361 agtacaaacg ctggggaggg atccgtgaag gggtgacact gacggaggcg cagaagaaac
    12421 agattaacaa tgttgtgggg gcagatgttt cttcaacgtt gtttgccacc ggctattacc
    12481 tgtatatcgg cgatatgctt ccctctttgc gggcaccccg tagcagtccg tcctgtacgc
    12541 tctggtactg tgacggcggt agtatccaga aacttgttat tgcatccacg gaggtccagt
    12601 aaatgtcggg taacaacaac accatcactg cggcggatgc cattatcacg ctgacagtga
    12661 ataatctgta tccctccggc gtacgacttc agggatttgc aacagataac gtttatggca
    12721 ccgatccact ggtactggcg gaaactgccc gcggtattga cggtaaactg tccgcgggat
    12781 ttgtgtacag caacattatc cagacgtttc atatcatgcc ggattccccc agccgggata
    12841 tttttgatac ctggtcaacc acatcccggg ccagtcgggc tgtattccgt tgtaacgctg
    12901 tcgtcctgct tccggcgatt ggccgtaaat atacctgcgt gaatggcgta ctcaaacaat
    12961 ggaaagcgct gcctgatgca gctcgtacgt tgcagccagg gcaggcggtt attgagtggg
    13021 aaaccattac tccggaggtt tttaactgat ggcccgtaaa gagaaattta tcactattga
    13081 aggtcaggga cgggataagg gcaaggtatt tcatcttacc gaaatgtctg cctcacaggc
    13141 ggaatggtgg gcgatgcgtg ccattatggc aatggggcgt ggtggcgtgg agttaccgga
    13201 tgatgttcgt agtctgggga tggctgcgct tgcccttgaa gggctgaaag cattatcgaa
    13261 aattccgcct gatgaggcgc gtccgttgat ggatgaaatg atggactgtg tgcagtttgt
    13321 tcccgatccg aaaaaacgca gcatccggag accgcttgtt gaagaggata ttgaagaaat
    13381 cattacccgt tttaatctgc gtgcggaggt ctttaagctg catgtggatt ttttcgctac
    13441 cgtcgtcagc tagatatccc cccgtgttat ctcgaccccg acagaccgtt cggggtggtg
    13501 gattacgtta atgttccccg taccattgcg accgttatct cctccggtaa ggcttcaaaa
    13561 gctgaactgg attctgtcct tggtgtgcag gacttatggg atctgctgga aataattcat
    13621 gtggatgccc ataacgaaca ggtgatacag gagaacagaa atggcgcagg tacttgatga
    13681 gcttgttctg tccctcggga ttgatgacag ggactttact gc
//
LOCUS       DBKEKI010000098        13619 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.098, whole genome shotgun sequence.
ACCESSION   DBKEKI010000098 DBKEKI010000000
VERSION     DBKEKI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.098"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            66..491
                     /gene="tnpA"
                     /locus_tag="ACUQPX_002928"
                     /pseudo
     CDS             66..491
                     /gene="tnpA"
                     /locus_tag="ACUQPX_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604943.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(605..952)
                     /gene="grxD"
                     /locus_tag="ACUQPX_002929"
     CDS             complement(605..952)
                     /gene="grxD"
                     /locus_tag="ACUQPX_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HHV9057579.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1287..2114
                     /gene="mepH"
                     /locus_tag="ACUQPX_002930"
     CDS             1287..2114
                     /gene="mepH"
                     /locus_tag="ACUQPX_002930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HHV9057580.1"
                     /translation="MARINRISITLCALLFTTLHLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2242..2823
                     /gene="sodB"
                     /locus_tag="ACUQPX_002931"
     CDS             2242..2823
                     /gene="sodB"
                     /locus_tag="ACUQPX_002931"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HHV9057581.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2969..4138)
                     /locus_tag="ACUQPX_002932"
     CDS             complement(2969..4138)
                     /locus_tag="ACUQPX_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9057582.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTPGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4304..4393)
                     /gene="cydH"
                     /locus_tag="ACUQPX_002933"
                     /gene_synonym="ynhF"
     CDS             complement(4304..4393)
                     /gene="cydH"
                     /locus_tag="ACUQPX_002933"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HHV9057583.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            4692..5717
                     /gene="purR"
                     /locus_tag="ACUQPX_002934"
     CDS             4692..5717
                     /gene="purR"
                     /locus_tag="ACUQPX_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HHV9057584.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5714..6646)
                     /gene="punR"
                     /locus_tag="ACUQPX_002935"
     CDS             complement(5714..6646)
                     /gene="punR"
                     /locus_tag="ACUQPX_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HHV9057585.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            6759..7970
                     /gene="punC"
                     /locus_tag="ACUQPX_002936"
     CDS             6759..7970
                     /gene="punC"
                     /locus_tag="ACUQPX_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HHV9057586.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8261..9409
                     /gene="cfa"
                     /locus_tag="ACUQPX_002937"
     CDS             8261..9409
                     /gene="cfa"
                     /locus_tag="ACUQPX_002937"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HHV9057587.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9449..10090)
                     /gene="ribE"
                     /locus_tag="ACUQPX_002938"
     CDS             complement(9449..10090)
                     /gene="ribE"
                     /locus_tag="ACUQPX_002938"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HHV9057588.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10305..11678
                     /gene="mdtK"
                     /locus_tag="ACUQPX_002939"
     CDS             10305..11678
                     /gene="mdtK"
                     /locus_tag="ACUQPX_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HHV9057589.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11719..12974)
                     /locus_tag="ACUQPX_002940"
                     /pseudo
     CDS             complement(11719..12974)
                     /locus_tag="ACUQPX_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            13282..13358
                     /locus_tag="ACUQPX_002941"
     tRNA            13282..13358
                     /locus_tag="ACUQPX_002941"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13316..13318,aa:Val,seq:gac)
     gene            13363..13439
                     /locus_tag="ACUQPX_002942"
     tRNA            13363..13439
                     /locus_tag="ACUQPX_002942"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13397..13399,aa:Val,seq:gac)
     gene            13547..>13619
                     /locus_tag="ACUQPX_002943"
     CDS             13547..>13619
                     /locus_tag="ACUQPX_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HHV9057590.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLK"
BASE COUNT         3297 a         3540 c         3247 g         3535 t
ORIGIN      
        1 cacgctcctg ttgaccaccg ggtcttaact ggaatttaaa tgcctgtaat cgtttcacag
       61 gtcaaatgat actttggtct gtgaaaaaag aaaccgatat ccggcgtgga aggcattgtg
      121 ttttcctgat gcatgttcac ctggtctttg tcaccaaata ccgacgccag atttttgacc
      181 atgacacgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgcagctg
      241 aactggttga aatggatggc gaaccagatc acgtccattt gttaattaac tatcctccca
      301 aactggcgat atccagtctg gtaaacagcc tcaaaggcat atcgaatagg ttactgcgac
      361 gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg agtcctggct
      421 attttgccag tagctgcgaa ggtgcgccaa tatccgtcat ccgccaatac attgaacaac
      481 agcaaacacc tggtcaggtg gaaaaccgcg ccttatatcc ccgccctgaa ggacgaggtt
      541 ttacggcgca ccggataaag gactgacgga tgcccgttcg catccgtcag tattgcagga
      601 cggattattc cgcgtccggc tcttcagact tgtatttagc ggcagtttct ttgatcagct
      661 gctgcagttc gccacgctga tacatttcga tcacgatatc acaaccgccg accagctcgc
      721 cgtcaaccca cagttgcggg aaggtcggcc agttagcata tttcggcagt tccgcacgaa
      781 tgtccggatt ctgcagaata tcaacatagg caaaacgttc gccacatgcg gcaagcgcct
      841 ggactgcctg ggcagagaaa ccgcagctcg gcagtttcgg tgaacctttc atgtacagca
      901 ggatcgggtt ttcagcaatc tggcgttgga ttttttcgat agtggtgctc attgtcttgc
      961 ttcctcaaac ttctttacgg cagtaatctg acattgtagc gggtcagtgc ggcatcggaa
     1021 aataacattt ccatcacgct tttgctattt tatccctttg ctctatttgt tgcatttcaa
     1081 atattcgttt cttctctgat gcgcggcaaa ctggcgtatt tataaccatt tttagctttc
     1141 actgctgcat ttttttgcgc tcgccaacga aacgtatttt ttaacaataa aagctattaa
     1201 ctttctcttc ttctatgcat tagaatcatc aagttttgta aatcagacgc aggcatgata
     1261 gacctgcctt aacagaggga cgctcagtgg cgcggataaa ccgtatttcg atcacgctct
     1321 gtgctttact ttttaccacc ctgcatttaa cgcctatggc ccatgcttca aagcaagcca
     1381 gggagagttc tgctaccact catatcacca aaaaagcaga taaaaagaaa agcacggcaa
     1441 ccaccaaaaa gacccagaaa gccagccaga aaacagcgaa aaaagccgcc agtaaaagta
     1501 cgaccaaaag caaaaccgct tcttccgtta aaaaatcttc cattaccgct tctaaaaacg
     1561 ccaaaactcg cagcaaacac gccgtcaata aaacggcctc agccagcttt accgaaaagt
     1621 gtaccaaacg taagggctat aaatcgcatt gtgtgaaagt caaaaatgcc gcgtcaggaa
     1681 cccttgccga cgcgcacaaa gcgaaggtgc aaaaagccac gaaagtggca atgaataaac
     1741 tgatgcagca aattggtaag ccatatcgtt ggggcggcag ctcaccgcgt accggttttg
     1801 attgcagcgg cctggtttat tacgcttata aagatttggt gaaaattcgt attccgcgta
     1861 cggcgaatga aatgtatcac ctgcgtgatg caggacctat cgaacgtagt gagctgaaaa
     1921 acggcgacct ggtctttttc cgtactcagg gacgcggtac agccgatcat gtcggcgtgt
     1981 atgtcggcaa cggcaaattt attcagtcac cgcgcacagg tcaggaaatt caaatcactt
     2041 ctctcagtga agactactgg cagcgccact atgttggcgc tcgtcgggta atgaccccaa
     2101 aaacacttcg ctaaaacttt accctgttgt tacggcaaca gggtaagttc atcttttgtc
     2161 tcacctttta atttgctacc ctatccatac gcacaataag gctattgtac gtatgcaaat
     2221 taataataaa ggagagtagc aatgtcattc gaattacctg cactaccata tgctaaagat
     2281 gctctggcac cgcacatttc tgcggaaact atcgagtatc actacggcaa gcatcatcag
     2341 acttatgtca ctaacctgaa caacctgatt aaaggtaccg cgtttgaagg taaatcactg
     2401 gaagagatta ttcgcagctc tgaaggtggc gtattcaaca acgcagctca ggtctggaac
     2461 catactttct actggaactg cctggcaccg aacgccggtg gcgaaccgac tggaaaagtc
     2521 gctgaagcta tcgccgcatc ttttggcagc tttgccgatt tcaaagcgca gtttactgat
     2581 gcagcgatca aaaactttgg ttctggctgg acctggctgg tgaaaaacag cgatggcaaa
     2641 ctggctatcg tttcaacctc taacgcgggt actccgctga ccaccgatgc gactccgctg
     2701 ctgaccgttg atgtctggga acacgcttat tacatcgact atcgcaatgc acgtcctggc
     2761 tatctggagc acttctgggc gctggtgaac tgggaattcg tagcgaaaaa tctcgctgca
     2821 taataactga tggcaaatgc agcattgcct gatgcgcttc gcgcgccatg ccaacgcgga
     2881 tgaggcgtta acagcgcatt cggcaagata aatcgcacgt tgtcagcaac tgtaacgcag
     2941 aaggttatcc ttctgcgttt ttgtttaatt agctgttagc aacgcaaact gtttcaggtt
     3001 gttttctggc tgacataaac accagcaata atgccagtcc cgcgacaatc gcccccgtca
     3061 ccggcacaaa gctgtatccc agcccagcgg aaattaccgc accaccagca gctgctccca
     3121 gcgcatttcc aagattaaag gcaccaatat tgactgatga agacagacct ggcgcttcac
     3181 tggcgacatg catcacgcgc atctgtaacg gaggtacaac cgcaaaggtt gctgcgcccc
     3241 acaccaccat gctaatagct gcgccgaact cattgcgggc caggaacggg attgccagca
     3301 taatcaccat cagcagcaac aaaaagcctt tcaacgtgcc gttaactgaa cgatctgcca
     3361 gtttgccgcc gagatagtta ccgatagaga atccgacacc aatcagcacc agcattgccg
     3421 tgacgaacac cggtgttgcg tgggtaatac tttgcagtac cggagagata taggtgtaga
     3481 gggtaaacat tgcaccagct cccagtaccg tcgtcagcaa tgcagacagc acctgcggac
     3541 gcattaatac cgccagctct tttttcactt caggtcgtgc ccctgtacca cctttaggta
     3601 atgagaagaa cagacttacc attgaaatca ctcccagccc cgccgttgcc agaaatgaca
     3661 tccgccagcc gatggtttca cccaaccaag tcgccgccgg cacgccaccg atatttgcca
     3721 gggttaaccc cataaacata gtggcaactg cgctggcctg tttatgtttt ggcaccacgc
     3781 ttgcggccac gactgaaccc aaaccaaaaa atgctccgtg attcaggctg gtcaaaatac
     3841 gtgaaagcat cagggtcata taatccggcg cgatggcgga aagaacgttg cctggcgtga
     3901 aaattgccat caggaaaatc aacgcactgc ggcgggcacg atgagaaagt agaagcgtca
     3961 tcagcggcgc gcccaccatt acgccaactg cataggcact gattaacatt ccggcagcgg
     4021 gaatcgagac atccacaccg cgcgcaatga cgggcaacaa gcccattggc gagaactccg
     4081 ttgtcccgat accaaacgcg ccaatcgcca gcgccagcaa cggatagtta attttcatgc
     4141 cttatctcca cctcttcgcg tcattacgcg atattcatta aagtggcgaa agcatgacag
     4201 caatcacaaa aaaatgaaaa taacaaaaag agaaaacact tttgccattt tgctaacaaa
     4261 caggaaggag atgcgaggga gaacgcgctc cctcgagagg aaatcagtgc agcgcggcag
     4321 tcaaacccac ggctacgatc aaaccgagga cgataatcgt tgttaccagt gaaaatttaa
     4381 ggtcggtgct catcaagttt tctccttttt tattaccaca caaaaagtga tattacgcat
     4441 ttttacacac tgtgatgaaa aaatctcccg tcatttataa tgataagtgt ttttaccact
     4501 tccccttttc gtcaagatcg gccaaaattc cacgcttaca ctatttgcgt actggccatt
     4561 gaccccttcc tgacgctccg tgtcgttttt ccggcgtacc gcaacacttt tgttgtgcgt
     4621 aaggtgtgta aaggcaaacg tttaccttgc gattttgcag gagctgaagt tagggtctgg
     4681 agtgaaatgg aatggcaaca ataaaagatg tagcgaaacg agcaaacgtt tccactacaa
     4741 ctgtgtcaca cgtgatcaac aaaacacgtt tcgtcgctga agaaacgcgc aacgccgtgt
     4801 gggcagcgat taaagaatta cactactccc ctagcgcggt ggcgcgtagc ctgaaggtta
     4861 accacaccaa gtctatcggt ttgttggcga ccagcagcga agcggcctat tttgccgaga
     4921 tcattgaagc agttgaaaaa aattgcttcc agaaaggtta caccctgatt ctgggtaatg
     4981 cgtggaacaa tcttgagaaa cagcgggctt atctgtcgat gatggcgcaa aaacgcgtcg
     5041 atggtctgct ggtgatgtgt tctgagtacc cagagccgtt gctggcgatg ctggaagagt
     5101 atcgccatat cccaatggtg gtgatggact ggggtgaagc aaaagctgac ttcaccgatg
     5161 cggtcattga taacgcgttc gaaggcggct acatggccgg gcgttatctg attgaacgcg
     5221 gtcaccgcga aatcggcgtc atccccggcc cgctggaacg taacaccggc gcaggccgcc
     5281 ttgccggttt tatgaaggcg atggaagaag cgatgatcaa ggtgccggaa agctggattg
     5341 tgcagggtga ctttgaacct gaatccggtt atcgcgccat gcagcaaatc ctgtcgcagc
     5401 cgcatcgccc tactgccgtc ttctgtggtg gcgatatcat ggcaatgggc gcactttgtg
     5461 ctgctgatga aatgggcctg cgcgtcccgc aggatgtttc gctgatcggt tatgataacg
     5521 tgcgcaacgc gcgctatttt acgccggcgc tgaccacgat ccatcagcca aaagattcgc
     5581 tgggtgaaac agcgttcaac atgctgttgg atcgtatcgt caacaaacgt gaagaaccgc
     5641 agtccattga agtgcatccg cgtttgattg aacgccgctc cgtggctgac ggcccgttcc
     5701 gcgactatcg tcgttaatca cccgttgcgg gagtctcttc cggctcccgc agccactcct
     5761 tattcagcgt ctcactatcg ccgagatact caagcagcca ggttaacgca ggcgacatat
     5821 cattttgctg ccatgtcaga caacatgccg aatccgggaa ggggttttcc agttcaagtg
     5881 ctacccactt cccctcatta agccacggtt tggcgaaatg tgttggcacc atccctatgc
     5941 ataatcctgc cgagatacag gttgccgatg attcccagtc aggtacgaca agtctttttt
     6001 ggttatccag caaccaggta atacgtttag gtagcgttcg cgaggtgtct tcgcgcacca
     6061 acgacggcca gttgcgcaac gtatcatcgc tgaacgggcc atccatcaac gccagcgggt
     6121 ggtgactggc aacaacgcaa ctccagctta gcatccccat atcccggaag gcataacgac
     6181 cgcctaccgg aatcgcgcgt gttgcgccaa tcgccagttc cacgcgcccg tcggaaagcg
     6241 catcccagac accgttgaac acttcctgaa agacaagaag ttcgacatca tcaaaatggc
     6301 gataaaaatc aacgatcatc tgccgtgtac gttctggcct gacaatatta tccactgcga
     6361 tagctaactg accgcgccag ccgttcgcta tctgctgaca ttgctggcgg gtgatctgca
     6421 tttttttgac aacagagcgc ccttctttga gaaaccacgc tccagcagcg gtcagctcca
     6481 catcacggtg ccgtcgttca aagagcggta ccgccagcca ctcttccagc tgacgcacgg
     6541 tatagctgac cgcagaagga acgcgatgca gctcctgtgc cgcagcgcta aaactaccat
     6601 tacgcgctac cgcatcaaca acttcgagtg aatattctga ccacatagtc tgcctgcaaa
     6661 atttttgaaa ccagtcatca aatattaccg tttcacaaca ctaatttcac tccctacact
     6721 ttgcggcggt gtttaattga gagatttaga gaatatacat gcaacctggg aaaagatttt
     6781 tagtctggct ggcgggtttg agcgtactcg gttttctggc aaccgatatg tatctgcctg
     6841 ctttcgccgc catacaggcc gacctgcaaa cgcctgcgtc tgctgtcagt gccagcctta
     6901 gtctgttcct tgccggtttt gccgcagccc agcttctgtg ggggccactc tccgaccgtt
     6961 atggtcgtaa accggtgtta ttaatcggcc tgacaatttt tgcgttaggt agtctgggga
     7021 tgctgtgggt agaaaacgcc gccacgctgc tggtattgcg ttttgtacag gctgtggggg
     7081 tctgcgccgc ggcggttatc tggcaagcat tagtgacaga ttattatcct tcacagaaag
     7141 tcaaccgtat ttttgcgacc atcatgccgc tggtgggtct gtctccggca ctggctcccc
     7201 tgttaggaag ctggctgctg gtccattttt cctggcaggc gattttcgcc accctgtttg
     7261 ccattaccgt ggtgctgatt ctgcctattt tctggctcaa acccacgacg aaggcccgta
     7321 acaatagtca ggatggtctg acctttaccg acctgctacg ttctaaaacc tatcgcggca
     7381 acgtgctgat atacgcagcc tgttcagcca gtttttttgc atggctgacc ggttcaccgt
     7441 tcatccttag tgaaatgggc tacagcccgg cagttattgg tttaagttat gtcccgcaaa
     7501 ctatcgcgtt tctgattggt ggttatggct gtcgcgccgc actgcagaaa tggcaaggca
     7561 agcagttatt accgtggttg ctggtgctgt ttgctgtcag cgtcattgcg acctgggctg
     7621 cgggcttcat tagccatgtg tcgctggtcg aaatcctgat cccattctgt gtgatggcga
     7681 ttgccaatgg cgcgatctac cctattgttg tcgcccaggc gctgcgtccc ttcccacatg
     7741 caaccggtcg cgccgcagcg ttgcagaaca ctctgcaact gggtctgtgc ttcctcgcaa
     7801 gtctggtagt ttcctggctt attagtatca gcacgccatt gctcaccacc accagcgtga
     7861 tgttatcaac agtagtgctg gtcgcgctgg gttacatgat gcaacgttgt gaagaagttg
     7921 actgcccgaa tcatggcaat gccgaagtcg ctcatagcga atcacactga tctatatcga
     7981 tatacttata cttaggctgc taacaaaatt ttgttgtatg attgaaatta gcggcctata
     8041 ctaatttcga gttgttaaag ctacgataaa tattatgttt ttacggggac aggatcgttc
     8101 ccgactcact atggatggtc atttcggcaa gggttcctcc tttccctctg ttctacgtcg
     8161 gattatagac tcgcggtttt ttctgcgaga tttctcacaa agcccaaaaa gcgtctacgc
     8221 tgttttaagg ttctgatcac cgaccagtga tggagaaact atgagttcat cgtgtataga
     8281 agaagtcagt gtaccggatg acaactggta ccgtatcgcc aacgaattac ttagccgtgc
     8341 cggtatagcc attaacggtt ctgccccggc ggatattcgt gtgaaaaacc ccgatttttt
     8401 taaacgcgtt ctgcaggaag gctctttggg gttaggcgaa agttatatgg atggctggtg
     8461 ggaatgtgac cgactggata tgttttttag caaagtctta cgcgcaggtc tcgagaacca
     8521 actcccccat catttcaaag acacgctgcg tattgccggc gctcgtctct tcaatctgca
     8581 gagtaaaaaa cgtgcctgga tagtcggcaa ggagcattac gatttgggta atgacttgtt
     8641 cagccgcatg cttgatccct tcatgcaata ttcctgcgct tactggaaag atgccgataa
     8701 tctggaatct gcccagcagg cgaagctcaa aatgatttgt gaaaaattgc agttaaaacc
     8761 agggatgcgc gtactggata ttggctgcgg ctggggcgga ctggcgcact acatggcatc
     8821 taactatgac gtaagcgtgg tcggcgtcac catttctgcc gaacagcaaa aaatggctca
     8881 ggaacgctgt gaaggcctgg atgtcaccat tttgctgcaa gattatcgtg acctgaacga
     8941 ccagtttgat cgtattgttt ctgtggggat gttcgagcac gtcggaccga aaaattacga
     9001 tacctatttt gcggtggtgg atcgtaattt gaaaccggaa ggtatattcc tgctccatac
     9061 tatcggttcg aaaaaaaccg atctgaatgt tgatccctgg attaataaat atatttttcc
     9121 gaacggttgc ctgccctctg tacgccagat tgctcaatcc agcgaacccc actttgtgat
     9181 ggaagactgg cataacttcg gtgctgatta cgatactacg ttgatggcgt ggtatgaacg
     9241 attcctcgcc gcatggccag aaattgcgga taactacagt gaacgcttta aacggatgtt
     9301 tacctattat ctgaatgcct gcgcaggtgc tttccgcgcc cgtgatattc agctctggca
     9361 ggtggtgttc tcacgcggtg ttgaaaacgg ccttcgagtc gctcgctaaa ggctattcta
     9421 tcgccccctc ttcgggggcg agttcagatc aggcttctgt acctggttga ttcatggcat
     9481 tttctcgtgc cgccagcaca cgttctaccg tatctaccac tgcctgagtt tgtggatcga
     9541 tttcaatgtt gacgcgtgcg ccaagttttt tcttcccaag agtcgtgcgt tccagtgttt
     9601 ccggaattaa atggacgcaa aaacgcgttg gcgtgacttc gccgacggtc aggctaatac
     9661 cgtcgatgcc aataaatcct ttgtacagga tatatttcat caactgacta tcctggactt
     9721 taaaccagat ctggcgatta ttttccgagg ttaatatttt cgccacttca gcggtggtca
     9781 taatatgacc tgacattaag tgtccgccaa tttcatcact gaatttcgcc gcacgctcaa
     9841 cgtttaccca atcccccact tttaaatcgc caagattggt aatgcgtaac gtttctttca
     9901 tcaggtcaaa actgacatgg ttgccgttaa tttccgtcac ggtcaggcag caaccgttat
     9961 gcgccacgga agcaccggtt tccaggccgt ccagcatgtg gtcgggtaac tccaccacat
    10021 gcgtacgaaa atttggtttc tcgtcaatcg acaccagttt tgcggtgccc tgtacaatcc
    10081 ccgtaaacat acttacaact cctgaaatca gttaagacat tctgttcagc acaatagcag
    10141 gtggaaaacg cccttaccag tgaaggggta agaatggcta ttttttcact ggagaattaa
    10201 taaatcctcg ctacaataga ctgaatttcc cctgcttctt ctttttgctg cccattcagg
    10261 cggcttttta gtctctcata taactacaaa taaaaggtgt tcacgtgcag aagtatatca
    10321 gtgaagcgcg tctgttatta gcattagcaa tcccggtgat tctcgcgcaa atcgcccaaa
    10381 ctgcgatggg ttttgtcgat accgtgatgg cgggcggcta tagtgccacc gacatggcgg
    10441 cggtcgctat cggtacttct atctggcttc cggcgatcct ctttggtcac ggactgctgc
    10501 tggcattaac gccggttatc gcgcaattaa atggttccgg tcgacgtgag cgcattgcgc
    10561 atcaggtgcg acaaggtttc tggctggcag gttttgtttc cgttctcatt atgctggtgc
    10621 tgtggaatgc aggttacatt atccgctcca tggaaaacat cgatccggct ctggcggaca
    10681 aagccgtggg ttatctgcgt gcgttgttgt ggggcgcgcc gggatatctg ttcttccagg
    10741 ttgcccgtaa ccaatgtgaa ggtctggcaa aaaccaagcc gggtatggta atgggcttta
    10801 tcggcctgct ggtgaacatc ccggtgaact atatctttat ttatggtcat ttcggtatgc
    10861 ctgagctcgg tggcgttggt tgtggcgtgg ctactgcggc ggtgtattgg gtcatgttcc
    10921 ttgccatggt ttcttacatt aaacgcgccc gctccatgcg cgatattcgt aacgaaaaag
    10981 gcaccgctaa gcccgatcct gcggttatga aacgactgat tcaactcggt ttgccgattg
    11041 cgctggcact gttctttgaa gtgacactgt ttgccgtcgt ggctctgtta gtgtctccgc
    11101 tcggtattgt tgatgtcgca ggacaccaga ttgccctgaa ctttagttca ctgatgtttg
    11161 tgcttccaat gtcgctggcg gcagcggtaa ctatccgcgt aggttatcgt ctgggtcagg
    11221 gctcaacgct ggatgcgcaa accgctgcgc ggaccgggct tatggtgggt gtctgtatgg
    11281 caaccctgac ggccattttc acggtttcac tgcgggagca aatcgccctg ttgtacaacg
    11341 acaatcccga ggttgtaacg ctggctgcgc atttgatgtt gctggcggcg gtatatcaga
    11401 tttctgactc aatccaggtg attggcagtg ggattttgcg tggttataaa gatacgcgtt
    11461 ccattttcta tatcaccttt acagcttact gggtgctggg cttgccaagc ggctatattc
    11521 tggcactgac cgatctggtc gttgaaccta tggggccagc aggcttctgg ataggcttta
    11581 ttatcggcct gacatcggca gccattatga tgatgctgcg tatgcggttc ctgcaacgta
    11641 tgccgtcagc catcattctg caacgagcgt cccgctaata aagacaaggc gcaaccttca
    11701 cggcttgcgc ctgtgttttt acgcaggctg gagcgttgcg ccaatcccgt cttcgtctgg
    11761 ctgtaatttc agagcgttac acagagtttg ccgactggca tctgttatct caacgcgtat
    11821 atccccgtta acttcccctt tcacattgat gaagtcaggt gaaatttctt tcgcactggt
    11881 ttgcaggcaa atcgctccct ctccgcgcag gcgtttcact ttcagttgca catgcgttgc
    11941 cgcaccagtg atcagccaca gttttccgcc attcagattg atgttaccaa caatgaaagt
    12001 gatgctgtca ccttttaccg tactgccgtc gagaatcaac acaccgccct gctgtacagt
    12061 aatcgttgat tgttgcagct ctgcgccata accaataatc attgaagcgc cgtccagtaa
    12121 tgcaacactc ccggtcagtt caaccggtgc gcgatcttcc tctacgacaa aattttcgtc
    12181 cgcgctggtg ggtgacaaca ggatcactgt tccaccttgt attaccgcat tttccagcgt
    12241 accggcatgc acttcagccc gccccccaca ttcaccgaca ggttttgcgt cttaccggaa
    12301 ctgtagagct gaaggccatc cgtccccaga cgataaatgg atccattctc aacaatagtg
    12361 tcagagtctt caccaccgag attattcatg gtgccaccag caagcaacgt atcactggct
    12421 ttcccggcca gcataaaaac gccgccctga ttaatctcac aattgctggc ttctccactt
    12481 ggatacacca acaattcacc accttcatct accacgaccg ctgaggttgt cccattaacc
    12541 aacaggccgc cctcttgatc gagaatgatt ttttccgcgc gatgtccttc cagtacacgc
    12601 aggttaccgc cattttccag caacaaaccg caggcataac cctgatcaac gctgaattca
    12661 ccttcgggat ggcggccatt aaccgtagcg agcgtagaga gactaattgc gcctccctcg
    12721 gactgaacaa tatctgtggc aatggcaccg tcgctgaccg atagcgtgcc attgttaccg
    12781 tactgtgtgc atttggcgat cccaccggtt gccactttca accagccagc ccccgtgacc
    12841 acggtatttt cggctcttcc gccagcttca acaatcaact cgccttcagt aagttgcgtc
    12901 gcgccaatga cctcgccggt cttaacaatt tgcacattcc cgtcgctcat caagtttttc
    12961 gcatcagatc ccatgattta ttcctttgct gcatctgtgt gcctttattg ctacctaagt
    13021 gtaaaggcta cggaggattt atccacgaca gatttgagat ggtggcaaac aactctgttt
    13081 aaactctgat acacgaatta ttgggttgta tcagatgtaa atgcgatcct gaataaaaat
    13141 cgcccttgca aatcaacaaa atatagccaa tttgcttaaa agctctgcaa acggtcgaaa
    13201 tagcgcagaa aattacgttt tgcctcttgc cacctttcca ctctgccgct aatattcgtc
    13261 cccgttgtca cctacaacgt tgcgttcata gctcagttgg ttagagcacc accttgacat
    13321 ggtgggggtc gttggttcga gtccaattga acgcaccatc ctgcgtccgt agctcagttg
    13381 gttagagcac caccttgaca tggtgggggt cggtggttcg agtccactcg gacgcaccag
    13441 attttcttaa tctggtcttt tcctttttcc ctctgtttct tctctgtatc caatacgtta
    13501 aaagatttac actgtcttcg tatgcgttat cagaaggaga atcgctatgg caactttgtt
    13561 acaacttcat tttgctttta atggcccatt tggcgacgca atggctgagc agcttaaac
//
LOCUS       DBKEKI010000099        13589 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.099, whole genome shotgun sequence.
ACCESSION   DBKEKI010000099 DBKEKI010000000
VERSION     DBKEKI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.099"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..893)
                     /gene="yafC"
                     /locus_tag="ACUQPX_002944"
     CDS             complement(<1..893)
                     /gene="yafC"
                     /locus_tag="ACUQPX_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HHV9057591.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPG"
     gene            1134..1934
                     /locus_tag="ACUQPX_002945"
     CDS             1134..1934
                     /locus_tag="ACUQPX_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HHV9057592.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2012..2782
                     /locus_tag="ACUQPX_002946"
     CDS             2012..2782
                     /locus_tag="ACUQPX_002946"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHV9057593.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYFAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2830..4188)
                     /gene="mltD"
                     /locus_tag="ACUQPX_002947"
     CDS             complement(2830..4188)
                     /gene="mltD"
                     /locus_tag="ACUQPX_002947"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HHV9057594.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4260..5015)
                     /gene="gloB"
                     /locus_tag="ACUQPX_002948"
     CDS             complement(4260..5015)
                     /gene="gloB"
                     /locus_tag="ACUQPX_002948"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HHV9057595.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5049..5771
                     /locus_tag="ACUQPX_002949"
     CDS             5049..5771
                     /locus_tag="ACUQPX_002949"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHV9057596.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5768..6235)
                     /gene="rnhA"
                     /locus_tag="ACUQPX_002950"
     CDS             complement(5768..6235)
                     /gene="rnhA"
                     /locus_tag="ACUQPX_002950"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HHV9057597.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITLWLHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6300..7031
                     /gene="dnaQ"
                     /locus_tag="ACUQPX_002951"
     CDS             6300..7031
                     /gene="dnaQ"
                     /locus_tag="ACUQPX_002951"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HHV9057598.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7164..7240
                     /locus_tag="ACUQPX_002952"
     tRNA            7164..7240
                     /locus_tag="ACUQPX_002952"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7198..7200,aa:Asp,seq:gtc)
     gene            complement(7371..8417)
                     /locus_tag="ACUQPX_002953"
     CDS             complement(7371..8417)
                     /locus_tag="ACUQPX_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HHV9057599.1"
                     /translation="MEIFLPDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8428..9423)
                     /gene="tssG"
                     /locus_tag="ACUQPX_002954"
     CDS             complement(8428..9423)
                     /gene="tssG"
                     /locus_tag="ACUQPX_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HHV9057600.1"
                     /translation="MSNDFTQAQEPPWRYGFLNLMRRVDVQLCTVPAGNTWQPRMEKF
                     RLGQTPALTFAPREIASVGWQEGRLHISLYSLGLWGPNGPLPLHYTELARNRTESRRD
                     PTLTRFSDLFHHRWLTQFYQAWRSAQSAGGGLDKPQHDRFAFYVASLSGQDLRESADS
                     PLPDHARLAASAHLVRESRNPDGLATTLAHYFGVPFRIQEYVLHWIAVADDEITRLGM
                     PAPSSVIGNGALIGQAVPDMQYKFRLVIGPLTLEDYRRFLPGSNNLPVLTELVRAFSG
                     YEYCWEIELQLKPHAAPPAVTGGPYQLGWTAWAGKAMHDNPVTGMIFEPEHYLAH"
     gene            complement(9420..11303)
                     /gene="tssF"
                     /locus_tag="ACUQPX_002955"
     CDS             complement(9420..11303)
                     /gene="tssF"
                     /locus_tag="ACUQPX_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HHV9057601.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11319..11813)
                     /gene="tssE"
                     /locus_tag="ACUQPX_002956"
     CDS             complement(11319..11813)
                     /gene="tssE"
                     /locus_tag="ACUQPX_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HHV9057602.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11810..12640)
                     /locus_tag="ACUQPX_002957"
     CDS             complement(11810..12640)
                     /locus_tag="ACUQPX_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HHV9057603.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTALACNARGEAQEADAHRSRALEVITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHRAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12627..13541)
                     /gene="tagK"
                     /locus_tag="ACUQPX_002958"
                     /pseudo
     CDS             complement(12627..13541)
                     /gene="tagK"
                     /locus_tag="ACUQPX_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3113 a         3543 c         3628 g         3305 t
ORIGIN      
        1 ccgggagctg tttttacatg ctcgctaagg aaatcgataa aagcccggat gcgcgtactt
       61 accgcacggt cgctgtaata gacggcactg aatggcattt ccactggcaa cactttatct
      121 gccattaact ccaccaattc tccgcgagcg atttctttgt cgatcatgta gtcggacaaa
      181 cacgcaatcc cgttgccact caggcaaagc tgtttcagtg tttccccact attggatgac
      241 aaaccgtact tcacctcatg taattgtcca tcactacagg ctatcggcca ggtattgagg
      301 gaagcgggtt cagtgaatcc caggcaaaca tgttgcttta aatcgtcgat cgtttctggc
      361 ttcccgtagc gggaaatata atcgggggag gcgataattt ttcgataact gttaaataac
      421 ggcctggcac gtaagctgga gtccgttaac gtaccagcgc gtatcgcgac atccactttt
      481 ctttcgatca aattaataat cgtttcggag gagactagcg ataaagtgac ttccggatag
      541 cgttcacgga aaggcttaat taacggcatc agaaagtgca gcaccactgg agttgcggca
      601 tcgatccgta acagtccacg cggtgtatta cgcgtctcca taatttctga ttctgccgct
      661 gccatctcct gcaaaattga ctgtacgcga cgaaaataac gctcgccttc ttccgtcagg
      721 ctaagttgcc gcgtggtccg attaagcagg ctaacgccaa gtttcatttc cagctttttc
      781 accgcccggc ttaccgctga gtttgcttgc cctaattgtt ccgctgcccg gctaaagctg
      841 ccgctttcga cgaccgaaac aaaaatggcg agttcttccg acgtggcttt catttttgct
      901 cctgttgcaa aatagatgag atattttgaa tttatttgtc attaaaccat caggatgcgt
      961 gatatgtcat ccgatttaat gttctcaata atgagcaaat ttctgaccgg tgtaagcact
     1021 tgcttacata acaatataca attgctcgtt gaaagagtga gctaaaatcc ctataacagt
     1081 agaaccctcc cgagtgcgga agggttgacg taatagaggt ttcaaagtca aaagtgcgaa
     1141 aaaacaccta tgccatgcgc tatgttgccg gacaacctgc ggaaaggatc ttaccgccgg
     1201 ggtcttttgc gagcatcggc caggcattac cacctggaga accgttaagt accgaagagc
     1261 gtattcggat cctggtgtgg aacatataca aacagcaacg cgctgaatgg ttgtcggtat
     1321 taaagaacta cggcaaagat gcacatctgg tgttattgca ggaagcgcag acaacgccag
     1381 agttagtaca gtttgcgacc gctaactatc ttgccgccga tcaggtgccc gctttcgtgc
     1441 tgccacaaca tccttccggt gtaatgaccc tttcggcggc gcatccagtg tattgctgcc
     1501 cgttacgtga acgagaaccc attttgcgtc tggcgaagtc ggcactggtg acggtctatc
     1561 cattacctga cacccgcctg ttgatggtgg ttaatataca cgccgtcaac ttcagtctgg
     1621 gcgtggatgt ctatagtaag cagttacttc ctattggtga tcagatagct caccacagcg
     1681 gcccggtcat tatggcggga gatttcaatg cctggagccg tagaaggatg aacgcgttat
     1741 atcgctttgc gcgggaaatg tcgctgcgcc aggtgcgttt taccgatgat cagcgccgtc
     1801 gggcgtttgg ccgcccgctc gattttgttt tctaccgtgg tctgaacgtc agcgaagctt
     1861 ctgtattggt tacgcgcgct tccgatcaca atccgctact cgttgaattc agtcccggca
     1921 agcctgataa ataaggtatg tcaggtctgc cacagggcag accaacgttt ggcgctgtcc
     1981 ttttatactc aaacaacaaa aggacagcgc aatgacaaca caatcccacc atgaccacgt
     2041 agaaaagcag tttagctctc aagcctgcga atatctgacc agtaccgtac atgcatccgg
     2101 acgagattta cagcgcctgg cggtgcgtct ggctgactat cctggtgcaa gtgtacttga
     2161 tatgggctgc ggagcagggc atgccagctt tgttgctgcg caaaacgtga gcgcggtggt
     2221 ggcgtatgac ttatctgccc aaatgctgga tgtcgtggca caagctgccg aagcccggca
     2281 actgaaaaat atcaccaccc gccagggata tgccgaaagt ctgccatttg ccgataacgc
     2341 atttgatatt gttatcagcc gttattttgc ccatcactgg catgatgttg gtgcagcact
     2401 gcgtgaagtg aaccggatat tgaaacctgg cggtaggatg attgtgatgg acgtaatgtc
     2461 tccgggtcat ccagtgcgcg acatctggtt acagacggta gaagcattac gcgatacctc
     2521 tcacgtacga aactacgcca gcggtgagtg gttgaggtta atcaatgaag ccaatctgat
     2581 agttgataat ttaattacag ataagttacc gctggaattt tgttcatggg tcgcgagaat
     2641 gcgtacgcca gaagcgttag tagacgctat tcgcatttac caacagagcg catcgacaga
     2701 ggtgagaacg tattttgcct tgcagaatga cggctctttc accagtgata tcatcatggt
     2761 agaagcacat aaagcggcat aaataaaaaa ggcaccgggg gaatcggtac ctttttatta
     2821 tctggtttgt caggaatctg gcatgttgtt gtttttcaca aacaacgtca gcttgtcgcc
     2881 tggttgcaga ttcgcagtgt cgctgttcca gcgcatcaca tctttgatgt tcacgccgtg
     2941 gcgtttagca atgcttgaaa gcgaatcgcc tttgcgcaca cgatacgtaa tgctatcgct
     3001 gttgtttgcc agtcgctgtg cgctactgcc tgcaccaatc gtcaaacttt ggcctggctt
     3061 cagcttagat ccgcgcagtt tgttccactg ctgcaaatct ttggtgctta cgccgagacg
     3121 tgaagcgatg cttgaaagcg tgtcgccaga gcgtacggtg taaacacggc tgttaagcgg
     3181 cgtattgtcg gcaaccagcg tcgactgtac agcagcaatt tcgcctgaag cgagagattc
     3241 acgcagttga tctgcatgct tctttggcac catcacgtac tgcggcccac ttgcgcccag
     3301 cgtggagcct ttcacgccag cgttgaatgt cttcagcttg ctgacggaaa tccccgccat
     3361 atctgcaacc ttcgccattt caaccgggct actcaggtgc acacgcgcca gagcacggct
     3421 ttcatcggtc gttggcagac gtacgccata acgcttgctg tttttgagaa tatcactcaa
     3481 tgccagcatt ttaggcacgt actgcttcgt ttcctgcggc aacggtaacg accagaagtc
     3541 cgtggatttc ccacgcgctt tgttcgtttt aattgccttc atgacccgac cttcgccgct
     3601 gttataagcc gctacggtca gaagccagtc gccgtcaaac attttgttca gacgctgcat
     3661 catattcagc gcggcagttg ttgaagcaac aacatcgcga cgcgcgtcat aattgcgggt
     3721 ctgtttcaaa ccataattgc gccccgtgct cggaatgatc tgccagatgc ctgcggcatt
     3781 ggcgccagac gttgcgtgag gatcaaaagc gctctccact atgggtagta gtaccagttc
     3841 cataggcatg ttacgttttt taacttgccc tgctatccag tacatatacg gctctgcccg
     3901 taaagttaca tcgtggagat agctcttatt gcgtaaatat ttctgtttct gttcgcgaat
     3961 ccggtcattt tccggaattc ccatctttag ctcgtcgcca atgaaagccc acaagtcacc
     4021 atctggcgcg atagacgtcc catcgtccat ccatcgtgcc tgacttgtaa actttgctgc
     4081 ttccccttga ccagctgcag aaaggctctg tgcgtgctgt tgaacgttgc cggtactctg
     4141 gcaacccacg agcaggacag aggcgagtaa tatcgctttt gccttcatgt gtgtgtcaat
     4201 agttgcttaa aagacgaccg atcataacgg cgaacggagc cgatgacaag aaagttttat
     4261 cagaacctat ctttctttga ccttaaccat gcaaaacgct cttcaggttg ttgcaataat
     4321 gtttcttcat taattacatt aattaaatca atatcttccg ttcttaaaaa aacattaatt
     4381 tgccgctcat ttttcagaat tacgggtagt gttatttgat tttttgcccg taactcctta
     4441 actttacgat aataatcatt tatggacaaa tcgtgcggaa aaatactcaa agcaaacttc
     4501 atatttgata acgtatattc atgagcacaa cataccaatg tatcgtcagg taacgcactt
     4561 agctttttaa gtgattgata catttgtaaa gctgtccctt caaacaaccg accacaccca
     4621 ccagaaaaca gcgtgtcgcc gcaaaataga taaggtttac tgaagtaaca gatatgtcct
     4681 aaagtgtgac ccggtgtggt aattacacta aattcatgcc ctaaaacgaa ggcagtttcg
     4741 ccatctttga ctacctgtgt tgttccctta tcttgtgtct cttgtggacc atacaccaca
     4801 atttgcggaa acttttccac cagttctttt acgccgccaa cgtgatcgtg gtgatggtgg
     4861 gttagaaata tggcctccgg ttgccagtta ttggcggtaa tggcgtttaa tactggctct
     4921 gcgtctccgg gatcgacaat caggcagcga cctgcctcat cattcaaaac ccagatgtaa
     4981 ttgtcatcaa aggcgggaat actgttaaga ttcatagatt acctctcagt gtaaaacgga
     5041 aggttgtgat gaaaccggca agagtccctc aaactgtcgt ggctcctgat tgctggggcg
     5101 atttgccctg gggagagctt tatcgcaagg cgctggagcg ccagctcaac ccgtggttca
     5161 ctaaaatgta tggttttcat ctgcttaaga ttggcaattt aagcgcagaa atcaattgcg
     5221 aagcgtgcgc ggtttctcat caagtgaatg tttctgcgca aggaatgccc gtccaggtgc
     5281 aggcggatcc acttcatctt ccttttgccg ataaatccgt tgatgtttgt ctactggcac
     5341 ataccttgcc gtggtgcacc gatccgcatc gtttattacg tgaagccgat cgggtactga
     5401 ttgatgatgg ctggctggtg attagtggct tcaaccccat cagtttaatg ggattacgca
     5461 aacttgtgcc ggtattgcgc aaaacctcgc cctataatag ccggatgttt actctgatgc
     5521 ggcaactgga ctggctctct ttgttgaatt ttgaagtgct gcacgccagc cgtttccacg
     5581 ttctcccgtg gaacaaacac ggaggaaaac tattgaatgc gcatattcct gcgcttggtt
     5641 gcttacaact tattgttgcc cggaaacgga ctattccttt aacgctaaat ccgatgaaac
     5701 agagtaaaaa caagccacga attcgccagg cggttggagc cacccggcaa tgtcgtaaac
     5761 cacaggctta aacttcgact tggtagcccg tatcttccag tgtgggattc atcgccgcgg
     5821 cacgagccag ttcatcacag cgttcgtttt ccgggtgtcc ggcatggcct ttaacccatt
     5881 cccatttgat ttgatgctgc cccaatgcag catcaagacg ttgccagaga tcgacatttt
     5941 ttactggttt tttgtctgct gttttccagc cacgtttttt ccagttatgg agccacaggg
     6001 tgataccctg gcggacatac tggctgtcgg tactcaaaat gacttcgcaa tgttctttta
     6061 acgcctccag cgcgacaata gcggccatca actccatacg gttgttggtg gtgcgggtgt
     6121 agcctgcgct aaaggttttc tcgcgtccgc gatagcgtaa aatagcgccg taacccccag
     6181 gtccaggatt acccagacac gaaccatcgg tgaaaatttc tacctgttta agcatctctg
     6241 gtagacttcc tgtaattgaa tcgaactgta aaacgacaag tctgacataa atgaccgcta
     6301 tgagcactgc aattacacgc cagatcgttc tcgataccga aaccaccggt atgaaccaga
     6361 ttggtgcaca ctatgaaggc cacaagatca ttgagattgg tgctgttgaa gtagtgaacc
     6421 gtcgcctgac gggcaataac ttccatgttt atctcaaacc cgatcggctg gtggatccgg
     6481 aagcctttgg cgtacatggt attgctgatg aatttttgct cgataagccc acgtttgccg
     6541 aagtagccga tgagtttatg gactatatcc gcggcgcgga gttggtgatc cataacgcag
     6601 cgttcgatat aggctttatg gactacgagt tttcgttgct taagcgcgat attccgaaga
     6661 ccaatacctt ctgtaaggtc accgatagcc ttgcggtggc gaggaaaatg tttcccggta
     6721 aacgcaacag cctcgatgcg ctatgtgccc gctacgaaat agataacagt aaacgaacgc
     6781 tgcacggggc attactcgat gcccagatcc ttgcggaagt ttatctggcg atgaccggtg
     6841 gtcaaacgtc gatggctttt gcgatggaag gagagacaca gcagcaacaa ggtgaagcaa
     6901 caattcagcg cattgtacgt caggcaagta agttacgcgt tgtttttgcg acagatgaag
     6961 agctggcagc acatgaagcc cgtctcgatc tggtgcagaa gaaaggcgga agctgcctct
     7021 ggcgggcata aatacatgta aaaggcgcta aaaatagcga cttgggcgat ttttgcagca
     7081 aacgattcaa aagatgagaa aaatcgttga cgaaggtcga ggcaatccgt aatattcgcc
     7141 tcgttcccaa cggaacacaa cgcggagcgg tagttcagtc ggttagaata cctgcctgtc
     7201 acgcaggggg gcgcgggttc gagtcccgtc cgttccgcca atattcagaa agcccgaatc
     7261 agtaatgatt cgggctttcg tcgttttggt gaagtgagaa tttttcactc cgaagtaaaa
     7321 cggatatgat ttaaaaaatg gccagacatt ataaattctg gccacttctg ttactccagc
     7381 gcatcccatt tctccagcag ttctactgga atttcattta ttacctccga gacgcgtttt
     7441 ccgaccaggc gttctgcctg gcgcagcaaa ggaatggtcg ggctgctcgg ttcgctgtgc
     7501 tcaaaccagc gacggatgac acgcaggcgt tccagcgcgt cgtttcggtc gcggatctgc
     7561 cggggttctg cgccggtcat cggtgatatt tcctccgcga tatgcaccgg tagcgcaatc
     7621 tgctctgcct gcatctgttc agtatgaacg atggtggcgt tttccggctg ctcagcctgt
     7681 ggttgtggca ggatctccgg cgtggtgctc tgcactgctc ccggtaacag ggccagcagt
     7741 tgcgtcagac gggagaagtc cggtgcctgg tcattcagcg tttcacgcgc atggcgttgc
     7801 agccgctcag tggtctctgc tgcctggcga aaggcatcca gcggcagtgt gccgcgcgct
     7861 tccaggtctg ccagttgctg gcgtaccgat tctggtgcca gcgcatccgc cgggcgtaat
     7921 gcagatagcg aacgctccac atcccgcacc tgaaggcgca tggcagcatt atttgacagt
     7981 gtaatgccac gaatatccgc cattacacct tcgtggtcca gcaacgccgc cagcgcgtta
     8041 ctgcgtgcca ccgcagcatc ttcggcggtg atatcttcag tcgcgagcag ttgaggatgg
     8101 atggcgtccg ggtacgtgga gcacaacgtt tccagcagcg tgagcgcctc cgacagtccc
     8161 ctggcaccgg cctgctggat acggcagcgc agcaggacaa ccagaatgcg gatatctttg
     8221 ctgcgcatca gcagacgatg ggcgtcccgc tcaagctccg cccagttaat gttttccggc
     8281 gtactgacaa aatcgccata ttgcgcttct tcacgcggtg cggcgcgggt gaacagcagc
     8341 aggtattccg gatcgtactc cagatccggg ccgcaggggc attccgccga aattgggtca
     8401 ggcaggaata tttccattta attatcctca gtgcgccagg tagtgttctg gttcgaaaat
     8461 catgcccgtc accgggttgt cgtgcatggc tttacccgcc catgcagtcc aacccagttg
     8521 gtacggacca ccggtaacag ccggaggggc ggcgtggggc tttagctgta gctcaatttc
     8581 ccagcagtat tcataaccac taaaggcgcg gaccagttct gtcaggacgg gcaggttatt
     8641 gctgccaggc agaaaacgtc ggtaatcctc cagcgtcagc gggccgataa ccagccggaa
     8701 cttatactgc atgtccggca cggcctggcc gatgagtgca ccattgccaa taacggaaga
     8761 gggggcaggc atccccagcc gggtgatctc atcgtccgcc acggctatcc agtgcaggac
     8821 atactcctgt atccggaaag gtacaccgaa atagtgcgcc agcgtggtgg ccagaccgtc
     8881 cggattgcgg gactcacgca ccagatgggc cgatgccgcc agacgagcgt gatctggcaa
     8941 cgggctgtca gcgctctccc gcaaatcctg cccgctgagg ctggcgacat aaaaggcaaa
     9001 gcggtcatgt tgcggtttat ccagaccgcc cccagcggac tgggcgctgc gccatgcctg
     9061 gtaaaactgc gtcagccagc ggtgatgaaa aagatcagaa aaacgggtca gcgtcggatc
     9121 gcgtcggctt tcagtccggt tacgcgccag ttcggtgtag tgcagcggca gtggaccgtt
     9181 cggcccccac agcccgaggc tgtacagact gatgtgcagg cgcccttcct gccagcccac
     9241 actggcgatt tctcgtggcg caaaggtcag cgcgggcgtt tgtccgaggc gaaatttttc
     9301 catccgtggt tgccaggtgt tgcccgccgg aacggtacac aactgcacat ccacgcggcg
     9361 catcaggttc agaaaaccgt aacgccaggg gggttcctgc gcctgagtga aatcgttgct
     9421 catacgcttc cccgctgtcc cgccctgaca ggccaggtca taattttgcc gcgctgcatg
     9481 ctgtgcagcg tcatctgcga gaaggtatta atggaaacat ggcgggcgat atagtgttcc
     9541 agcaccaggc cgaacagata cggactgatg ccggaaaaac cttcttcatc caccgtcagt
     9601 tcacaactga cgccacgccc atagaccagt agtccggaac cgggcagacg gcgggtgacc
     9661 ggggtcgttt tacaaccaat caggctgcgc acctggcgcg attgcggact gtcatgagca
     9721 ggaataaaca ggttcagcag atctcgcaac gcctggccgc cggtacggtg atccagatcg
     9781 gccagcggca gataattaaa agataactgg cggatcaacc gccatgccat ctcacgttca
     9841 gccagcgggg gctgcggcga gcgtggcggg cggataagcc ctacgcccgc caccgggatg
     9901 gctgcatcca cggttagatc gtcccggcca ttgcgggcaa taagacaggg cagatcgcgg
     9961 ttagtgacca tggccgtgat ggtgatgtgg cgcagatttt ccgggtacgg cgcttcatac
    10021 tggtcaacca gcgacaggaa cacttccgag cccgtatagg gggtacgggt gccgtaacgg
    10081 cgggcgtttt ctgataagcg gcggggctcc cggcgcaggg aaaaataacg tccgtgattg
    10141 ccttcatcat tattgcgggt gtggtagagc gggcgaaagg ccatcttccg tgtggtgtcg
    10201 gtttccagcc cttccacttc ctggaccgag aacacctcat aatccagcgg atgggttctg
    10261 tccaccacca gatgctgttc cgtcgtgctg tgcgtgacat cgatccgggc ggtgacgcgg
    10321 ggaaacaggt tgataaccgg ggtacagaac aggctgaact gtgccgcatt tgtctggtgg
    10381 atcagccagt ccggcggcag gcggttcagc aggataacaa tctccgccac gccaccgtca
    10441 attttctgta atccggcaga caggcctgtc ggggtgaaaa aatagaagcg ttccggacag
    10501 gcaaaatatt catgcagcag gttatgaccg tgaaacacgt tccacgccag cggcagcagc
    10561 ccctgatccg gctccaggcc ttcgtacata acgggctgtt gcagattcac atccagcgcg
    10621 ccgtcaaaat gccccggaat gccagccagg gtcgcgatgg cgctggtatg tagaagttct
    10681 aacaggtgtg aggccgtacg ctcctcgccg cagagataaa acggcagcct gtccagtccg
    10741 gcaagctgac tgaacgttag ttcaccaaag gtgcgcaggg taatacgcag cgccccggcg
    10801 acgtgaatgt tggccggcag gtagcggtgc agggcaggca tatccggtgg cgcggcggtc
    10861 agtcgcgcct cttcgatggc gagcggccac agcgttacat cctggctgct gcgaaactgg
    10921 caggcagtgc tttcgccttc cggaatggcg gagaaaaagg ctgtgtcgcg cgggacggta
    10981 aatcctttcg ccagatcgcc ttcttccgta tcgggatgca actgcgccac gctcattgag
    11041 ggcgtgggtg tgacgtaatt ggggctgacc acttccagca ggcgctgggt gaaacggggg
    11101 aattcagcgt caatttttag ctgcgtgcgg gcggtcagga agctgaaggc ctcgaccatt
    11161 cgctctacat acggatcggc aatatccgtt ccctgcatgc ccagccgggc ggcgactttg
    11221 ggatgacggg cggcaaattc ggctcccgtt tcccgcagat agctcagttc gcggttgtag
    11281 tattccagta gccgtggatc catcggtctc tctctgaatt aaaaaaacgt aatgcgactc
    11341 tgctccatat ccagcgcgct acgtacccgg aatgcagtcg gatacggctg cgtgaggatc
    11401 tcgccgcgaa tttcaaactg taatgtgtta tggccactct ggcggcgctc atcctgaagt
    11461 gggatgattt gcagggtgga cgctttcagc cggggttcaa accgggtgat ggttcgcaaa
    11521 atggccctgc ggatatcgtc ccacttgtgc tcatacagaa agctgcccgc cagtggcggc
    11581 aggccgtagt taagtacgga cgctgccgcg tacggataac gtgcagcatc aatatcatct
    11641 tcatggctga cggtgttgag cagaaatgac aaatcccggc ggatgatctc cttaatctga
    11701 accgggctga cgctgatacc gtggtcgcgt ttctggtttg gggcgtcgtc acacaggcgg
    11761 tcaaacagcg tgggcagcag gtgattgcct ggacgctggc gggaagcgct cacgcgtcgc
    11821 ttccttcagc ggtgttaaag gtacaggtgg acaaatccag caggctgata tcgccgtgac
    11881 cggtgagcca caccctctga cccagcgcct gtaccagtgt ctctcccgga ccgtcctgcc
    11941 agaccgtttc ccggcacaga cgcagggagt cggcagcctt ttcagagccg ctgtagcggg
    12001 gaaacagcca ggcacggtgt gtatcgccat tgttcagggt gacgttgacg ggtttccaga
    12061 tcaaatcgat cagatgcgca ggccgcggcg atttcagcga acagatttgt gaaaacggca
    12121 gccagatata tgctccgccc gcgatcagtt cgagtaccgg accggtacgg gagtcgctgt
    12181 cgctaatcca gtcaaatgcg ccgccattcc actgcccgga agtgtctgtt atgacctcaa
    12241 gcgcgcggct gcggtgagca tcagcttcct gcgcttcacc acgggcgtta caggcgaggg
    12301 cggtcagcag atcctccatc caggcgggcg gcgggagaat aacaccgggc cgctgttcgc
    12361 cctgaaagca ggcgtggcgg tatatctcac agcggatcag ctcgccaaat acctgcgctt
    12421 cgcgagtata atttgcgtcc atccgggcgc atagctggat ctgttgcagt gcccgcgacc
    12481 agtcgcctgc cacgcacaat aactgaaaca ggctgtggcg gcgggaggcc tgcgctgggt
    12541 gcatcctgac ctgttgctct gccatctgaa tgccctccac cacggaataa tcccgcagca
    12601 gcgccgacaa ggtaatcggt agctcgttat tgtttgtcat gatatatctc tccgtgttgt
    12661 gccgggttta tccggtaatg gctgtcaatg ccaatgatgc gatgctcttt ccgtgtcaga
    12721 tctggcagta cggtgttatg cgctgtttcc ggggcgtatt ccggggaaag taaatgcagg
    12781 atatcagggg gagtcttttc gacaggccag atagtttcat cttcgccagt ggcatccagt
    12841 acattgagaa tagcgtcaat gcttaaccca ccagtgacca tatcctgcaa tgtctcggct
    12901 tcggtgacca ggtcacgcaa tgaatccaga tcttgcgagg agggctcttc gttaaaagga
    12961 aaaggatcag catggcgatt aagatcttgt tcgggagata acaacgcctc gcggtattcc
    13021 tgataaagtc gggtcagggc atttcctttt tccgcacctt ctccggtgga ggaggctcca
    13081 tgaacaggaa tgatatcgaa tggattttgt gatgtcagat gctgctgcct gaaccaatcc
    13141 agatccagat attcggttat tgcttccgac gtgatggcag tctgcgatat cgtttttact
    13201 gtgctcaggt ccggagatga tggattgcca tgtacaaggc actatgaaga taacccccat
    13261 tctatcaggt cgccttcatt cagacgcata cggtgatgag gttccacaat ctggtcgttt
    13321 accgtacagc aatattcggt agactgattg ataatccacc atgcttcttc gtggcgggtc
    13381 agagtgaggg caatctttac atcatcattc accttaaaaa caggaatcca gggagaggta
    13441 acggtgaaga taatttgtgt tccttcagtc gcgtaaattg tgttatcggc gatcccgtgc
    13501 gcctgtattt tttggagtcc ccacgtaata tttggtttca ttgtgtatag actggccccc
    13561 tgaatctcca gacaaccagt atcacttaa
//
LOCUS       DBKEKI010000100        13552 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.100, whole genome shotgun sequence.
ACCESSION   DBKEKI010000100 DBKEKI010000000
VERSION     DBKEKI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.100"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..247)
                     /gene="araH"
                     /locus_tag="ACUQPX_002959"
     CDS             complement(<1..247)
                     /gene="araH"
                     /locus_tag="ACUQPX_002959"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HHV9057604.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVA"
     gene            complement(262..1776)
                     /gene="araG"
                     /locus_tag="ACUQPX_002960"
     CDS             complement(262..1776)
                     /gene="araG"
                     /locus_tag="ACUQPX_002960"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HHV9057605.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRNNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(1846..2835)
                     /gene="araF"
                     /locus_tag="ACUQPX_002961"
     CDS             complement(1846..2835)
                     /gene="araF"
                     /locus_tag="ACUQPX_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HHV9057606.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            3632..4135
                     /locus_tag="ACUQPX_002962"
     CDS             3632..4135
                     /locus_tag="ACUQPX_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HHV9057607.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4215..4466)
                     /gene="yecJ"
                     /locus_tag="ACUQPX_002963"
     CDS             complement(4215..4466)
                     /gene="yecJ"
                     /locus_tag="ACUQPX_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HHV9057608.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(4581..4667)
                     /gene="azuC"
                     /locus_tag="ACUQPX_002964"
     CDS             complement(4581..4667)
                     /gene="azuC"
                     /locus_tag="ACUQPX_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HHV9057609.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            4930..5253
                     /locus_tag="ACUQPX_002965"
     CDS             4930..5253
                     /locus_tag="ACUQPX_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HHV9057610.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            5424..5921
                     /gene="ftnA"
                     /locus_tag="ACUQPX_002966"
     CDS             5424..5921
                     /gene="ftnA"
                     /locus_tag="ACUQPX_002966"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HHV9057611.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(5958..6197)
                     /locus_tag="ACUQPX_002967"
     CDS             complement(5958..6197)
                     /locus_tag="ACUQPX_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HHV9057612.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            6387..7598
                     /gene="tyrP"
                     /locus_tag="ACUQPX_002968"
     CDS             6387..7598
                     /gene="tyrP"
                     /locus_tag="ACUQPX_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HHV9057613.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(7660..8325)
                     /gene="yecA"
                     /locus_tag="ACUQPX_002969"
     CDS             complement(7660..8325)
                     /gene="yecA"
                     /locus_tag="ACUQPX_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HHV9057614.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(8521..8607)
                     /locus_tag="ACUQPX_002970"
     tRNA            complement(8521..8607)
                     /locus_tag="ACUQPX_002970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8571..8573),aa:Leu,seq:taa)
     gene            complement(8620..8693)
                     /locus_tag="ACUQPX_002971"
     tRNA            complement(8620..8693)
                     /locus_tag="ACUQPX_002971"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8659..8661),aa:Cys,seq:gca)
     gene            complement(8748..8823)
                     /locus_tag="ACUQPX_002972"
     tRNA            complement(8748..8823)
                     /locus_tag="ACUQPX_002972"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8788..8790),aa:Gly,seq:gcc)
     gene            complement(8975..9523)
                     /gene="pgsA"
                     /locus_tag="ACUQPX_002973"
     CDS             complement(8975..9523)
                     /gene="pgsA"
                     /locus_tag="ACUQPX_002973"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HHV9057615.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(9580..11412)
                     /gene="uvrC"
                     /locus_tag="ACUQPX_002974"
     CDS             complement(9580..11412)
                     /gene="uvrC"
                     /locus_tag="ACUQPX_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HHV9057616.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHSKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKTGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(11409..12065)
                     /gene="uvrY"
                     /locus_tag="ACUQPX_002975"
     CDS             complement(11409..12065)
                     /gene="uvrY"
                     /locus_tag="ACUQPX_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HHV9057617.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            12207..12278
                     /locus_tag="ACUQPX_002976"
                     /pseudo
     CDS             12207..12278
                     /locus_tag="ACUQPX_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            12361..12537
                     /gene="yecU"
                     /locus_tag="ACUQPX_002977"
     CDS             12361..12537
                     /gene="yecU"
                     /locus_tag="ACUQPX_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HHV9057618.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            12524..12748
                     /gene="yecF"
                     /locus_tag="ACUQPX_002978"
     CDS             12524..12748
                     /gene="yecF"
                     /locus_tag="ACUQPX_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HHV9057619.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(12781..13401)
                     /gene="sdiA"
                     /locus_tag="ACUQPX_002979"
                     /pseudo
     CDS             complement(12781..13401)
                     /gene="sdiA"
                     /locus_tag="ACUQPX_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
BASE COUNT         3456 a         3426 c         3071 g         3599 t
ORIGIN      
        1 aggcgacaga aaggtcaaag tcaccggaag caaggcagaa caacatgcca cacgccacca
       61 tccccgacat ggaaattgcc aggcccaacc ctttcatatt aatgaaggtg gcaaaatttg
      121 ggacaaaaat ggcacaggcg ataaagagca ccgcaaacac caccagcatg ccgtactgat
      181 cccagatacg cccgaagctg aatgacgact taggtgcgcc agaccccgat gtagaaacag
      241 aagacatcat actctcctta ctcaggcaac tgcctggctg actttaggca tcgcaaggct
      301 cagtgcctga cgctcatctg cctgctcgtg taacaattca ccggcgattt cgccttcccg
      361 catcaccaca atccggtcgg caacgccgag gacttcaggt aagtcgctgg aggcaaacag
      421 caccgccacg ccctgcgccg ccagcgcata aattacgtta tatatttcgt gcttagcgcc
      481 aacatcaatg ccgcgcgtag gttcatccag caaaatgacc ttcatctctt ccgataacca
      541 gcggcccaga atggcttttt gctgatttcc gcctgagaga ttcatgatca gttgctccgc
      601 gcccggcgtt ttgatgttga gcgaacgaat gtgctgatcg gcattgtttt cttcccaact
      661 gttgttgatt acacaaccgc cgagcacatg tttgcgtctg gcactgatgt tgatattgtt
      721 gcgaacggag tgcacgggaa taatgccttc cgctttgcga gcttccgggc agagcatcat
      781 gcctgcggca atggcgtggc tcggtttacg aatatcgatc ggctgttggt cgatataaac
      841 ctgaccggcg gtgatttgcg tcccgccaaa caagcctttc attaattcgc tacgccccgc
      901 ccctaccaga ccaaacagcc caacaatttc accactgcga accgccagac ttattggcgt
      961 acgcacgcct ggtgctttca cagcatcaag acgcaggcgt tcctcgccat aactacgagg
     1021 ttgccagccg tagatatcgc caatgtcgcg cccgaccatc gcctgcacca gcgcgtcgtg
     1081 gtcaacctgc tgcatatcgg taaaggtttt gacataacgt ccatctttaa agacagtgat
     1141 ggcatcgctg agggcaaata tttcttccat acggtgagaa acgtataaga tcacccgccc
     1201 ctcttttcgc agttcacgaa taacgcggaa aagattgccg atttcgcggg cagaaaggga
     1261 gctggttggc tcatcaaagg cgataatttt agcgttacgc gccagcgctt tggcgatttc
     1321 aaccatctgc cactgaccaa tggagagata tttcagcggc gtgtccgggt caatatccat
     1381 accaagatgt ttaagttgta aacccgcctc ataattcagc aatgagcgat tcacaatgcc
     1441 gcctttatgc ggcagctggc cgagatagat gttttccgcg acggtcattt ccggcacgag
     1501 atgcagttcc tggtaaataa tcgccacacc cgcgttaagt gctgcggtcg tgtcggaaaa
     1561 ggacatttcc tgcccattaa tcactacaga acccgtggtt ggcgcatagt tgccgctgag
     1621 gatttttaag agagttgatt ttcctgcgcc attttcaccc atcaacgcat gaacttgacc
     1681 ggcatagcag tcaaaactaa tatccgtcag cgccttaacg ccgggaaacg ttttaccgat
     1741 gccgcgaaat gagagatacg gggtagactg ttgcataacg tctccgtgaa tcactggtcg
     1801 ttcacacccc ctctgcatca tgcagagggg aatttttccg gcaaattact taccgcctaa
     1861 accttttttc tccagttctt ctttaaagtt gtcacgcgtg atcagtacca cgtctgtaac
     1921 ttcggtaaat tttggcgttt caacgtcttt tgctacccag ttgtaaagca tttcgctgga
     1981 tttatagcca tgtacgtccg ggcttggcag cagggaaccg tagaagccgg ttgcctgtgc
     2041 tttagacagt tcgcttactg catccacacc gttaatacca ataccgatga tatcggctgc
     2101 tttaaagccc tgaccttcgg ttgcacgtac gccgccaagt acggtgctgt cgttcatacc
     2161 gacgatcagc caatgtttaa cttccggatg ttgaaccagc attgagttgg cagcgtcaaa
     2221 tgcccccggg atgtcgttag atttggtagg tacctgataa atttgttttt ccgggaatcc
     2281 tgcagctttc agtgcgtcca tagaacccgt tgtacggcgg cgggcggtat ccagttcgtt
     2341 ggcggtaatc gccatcactg cgctttcttt gacatcccag ccacgtttct gcatctcttt
     2401 atacagttcc tggccctgac gttcgccaat tttagtcgcc gccatcatca ccagaggaac
     2461 ggtatccatt ggcttacctt tggcgttaac aaactggtca tccactgcaa tgactttcat
     2521 atcgtagcca cgcgctttcg cgacgatggc agagccgagt ttcgggtccg gagtacaaat
     2581 aacgaaacct tttgcgccac tggctgccag gctgtcgatc gcgttcaatg ttttttcgcc
     2641 atccggcacg gcaatcttaa taacctcaaa ccctaaatcc ttcccggctt tatcggcaaa
     2701 cttccattcg gtctggaacc acggctcttc cggttgcttc accagaaaac cgagcttcag
     2761 gttctccgcc atagcggatt gtgacataac ggctgccaga ccaatggctg ccagggcttt
     2821 agtaaatttg tgcatggttc tctccagctt tagtgtcgtt ttgtgtaggg caaaaacgaa
     2881 tgacattcgt taaattaatc ggaaaacaaa gcattacctt ttatctaaaa gataagtgac
     2941 cgtgttgaca tagttttagc gagaaattaa ttctccatag gagagcaata tcacatcgca
     3001 gaattacagt gagaacgtgc ataaatttag cgggaaaaga cataaggaaa agccaatttg
     3061 tcagacaaat tgttgaatgc acagcagatt aatccataag attagcctgg aaatccttgt
     3121 tgtctttggt acccatgcgg gatgtcttct ttttaaccag tcaataggcc gcattacctg
     3181 gcgttgagtt tttgaaatgg tgtaataacc acaactcaaa gatgtggaaa atgcgcgtca
     3241 ttcatttcgc cattaattat cactgtgctc attaattaac agaacacgta taatgagagc
     3301 catctcgcaa aaatgaaaaa acgttttata aaatcatcac ttcatcatga attcaaattc
     3361 atcgattaat atcaacaaga tacaaaaagc actatcatta aaattcattg catttacatt
     3421 gatttcacca atgaaatgta aaaatatata aacttgatga tttaagcatt ttcttataac
     3481 cgttcagacg ttattcttat ttcagatcat cgtcagaatt gactccacga tcacatttcg
     3541 gaccggcaga aaggaattat tctgcaaaca gtaattatgg tgttttgatt tatcttgcac
     3601 ctctccactt ctggatataa ggatattagg tatggcaacc gctggaatgc ttctcaaact
     3661 caactctcaa atgaaccgcg agttttacgc atccaatctc taccttcacc tgagtaactg
     3721 gtgttctgaa cagagtctga acggcaccgc cactttcctt cgcgcccagg cacagagtaa
     3781 tgtgacccaa atgatgcgca tgtttaactt tatgaagagt gtcggcgcta cccccatcgt
     3841 taaagccatt gatgttccag gtgaaaaact gaactctctg gaagaactgt tccaaaaaac
     3901 gatggaagaa tacgagcaac gttcaagcac gctggcgcag ttagccgatg aagcgaaaga
     3961 actgaatgat gattcaaccg tcaatttcct gcgcgatctg gaaaaagaac agcagcatga
     4021 tggcctgttg ctgcaaacca ttcttgatga agtgcgcagt gcgaaacttg cgggtatgtg
     4081 ccctgtgcag accgaccaac atgttctgaa tgtcgtgtca caccagctgc attgatcata
     4141 catcggcgct aatacattgc gccgatgaag tttttgagaa accgctgcct catctgtttg
     4201 aagcagcggt ttttttaatg ggattcaccc tgtggggtaa acttgagttc aatcagcgcg
     4261 atggcttttt ggattgcccg catggtgacc gggtctgcgg cagcgggatg atgagtaaag
     4321 tcgatattct tcagctgact ggacattttt tcacgaactt caacgggcgc gattacatcg
     4381 agaacatcca gaatttgttt gataaccaac tggcaagcta ccacatcaga aaccagttcc
     4441 tgatcggcat tcagcggctg ggacatcgta aactcctgat agcattttga aagccgttat
     4501 agtagcggct tcacatcttc agcgatagtc acatccaccg tcatcaggac acaaaaaaac
     4561 ctgccggagc aggttttttg ttatcggaac atattgcctg gcggtacgtc tttgaacgtc
     4621 ttgcaatagt tattgaacat acttttcagg attttgcgca gtttcatcgc ggcactccga
     4681 ccatttgtta tacaggtgtt attgtctttg cgcttataat atgacaacca tcacaaaaat
     4741 caatctttat gtgatacaaa tcacataaat acccctttaa tgttataaaa atgataatca
     4801 aaaaacagcc cccctatttc tgacacctac agatggcaag aaatagcgcc tgccaggcgt
     4861 cttttccggc cattgtcgca gcgctgtaac gcgtaaaata gtgctttctc ttactcttct
     4921 ggctggacca tgagacttct gattctgact ctttcattaa taacgctggc ggggtgtacg
     4981 gttactcgcc aggcccatgt gagcgaagtt gacgcggcaa caggtattgt acggttggtt
     5041 tatgaccagg cttttttgca gcatgcccat accgatcgtt atgtcagtcg cgggatcgcc
     5101 gatcgcgcat gccagcagga aggctatacc cacgcgatcc cctttggtca gccagtaggc
     5161 aactgtagcc tttttgcggg ttccttatgt ttgaatacgg aattcacttt gtcatatcag
     5221 tgccatcatt ccgccttccc tgtctttctc taaatatttt aatcagcgag gggatcttcg
     5281 ctgattaaag aaatagcggt aatgcgtttt attcccattc gtatttttaa taattgaagt
     5341 ttatatttta ccttttgcaa ataataaaat aacaaattat agtgacgcca cagcaacaaa
     5401 tataaccttt gtggagcact atcatgctga aaccagaaat gattgaaaaa cttaatgagc
     5461 agatgaacct agaactgtac tcttcactgc tttatcagca aatgagcgcc tggtgcagct
     5521 atcatacctt cgaaggtgct gccgcgttcc tgcgccgtca cgcccaggaa gagatgacgc
     5581 atatgcagcg tctgtttgat tacctgactg ataccggcaa tttaccgcgt attaataccg
     5641 ttgaatctcc gtttgctgaa tattcctcac ttgatgaatt attccaggaa acctataaac
     5701 acgaacaatt aatcacccag aaaattaacg aactggctca tgctgcaatg accaatcagg
     5761 actacccaac atttaatttc ctgcagtggt atgtttctga gcagcatgaa gaagagaaac
     5821 tgttcaaatc gattattgat aaattaagcc tggcaggcaa aagcggcgaa ggtctgtatt
     5881 ttatcgacaa agaactctct accctcgaca cacaaaacta atgctctccg gcggcaggtt
     5941 tctgccgccg tttcaaatca gtgacggcaa atcttactct gatcggtcga aaaaccttct
     6001 tctgaaggta taaatttgcc ttttgctgcc agaaacgcta ccagctctcc cgctgtcatc
     6061 ccttctgccg agcacgtgtg aaaacgtgcc tgttcaccaa aacgcgcttt aatcgcagct
     6121 tccagactgg catgcgtata ttgctcgcct gattcaatca tcatatttaa cacttcatga
     6181 ccgtgaatag agtccatcgt tcctcctcaa aaaaagccta gcgtggcgat tacctcttat
     6241 gaagactttg cgccagcgca ggactgaatg cttttattgt acatttatat ttacacaata
     6301 tgtaacgtcg gtttgacgaa gcagccgtta tgccttaacc tgcgccgcag atatcactca
     6361 taaagatcgt caggacagaa gaaagcgtga aaaacagaac cctgggaagt gtttttatcg
     6421 tggcgggaac cacaattggc gcaggcatgc tggcaatgcc gctggctgcg gccggtgttg
     6481 gttttagcgt tacgttaatc ttgttgattg ggctttgggc gttgatgtgc tacacggcgc
     6541 tattactgct ggaggtgtac cagcatgttc cggcagatac cggtctgggc acgctggcaa
     6601 aacgctatct gggacgctac ggtcaatggc tgacgggctt cagtatgatg ttcttaatgt
     6661 atgctctgac tgcggcatac atcagcggtg ccggtgaatt gttggcctcc agcatcagcg
     6721 actggacagg tatttcaatg tcggcaaccg ctggcgtgct gttgttcact tttgttgccg
     6781 gtggcgtggt ttgtgttgga acatcgctgg tcgatttatt taaccgtttt ctgttcagcg
     6841 ccaaaattat ttttctggtg gtgatgctgg tattgctgct accgcatatt cacaaagtga
     6901 atcttttaac cctgccgttg caacaggggc tggcactgtc tgcaatcccg gtgattttta
     6961 cgtcgtttgg ttttcacggt agcgtgccga gtattgtcag ctatatggat ggcaacattc
     7021 gtaagctacg ctgggtgttt ataatcggta gtgccatccc cctggtggca tatattttct
     7081 ggcaggtggc gacgcttggc agcattgatt caacaacctt tatgggattg ctggctaatc
     7141 atgctggatt aaacgggctg ttacaggcgt tacgcgaaat ggtggcctct ccgcatgttg
     7201 agctggcggt acatttattt gctgatttag ccctcgccac gtcatttctc ggcgttgcgt
     7261 taggcttatt tgattatctg gctgatttgt ttcagcgttc aaataccgtt ggtggacgat
     7321 tgcaaactgg tgcaattacc tttctgccgc cgttggcgtt tgcactgttt tatccacgag
     7381 gatttgtgat ggcgctgggt tacgccggtg tggcgctggc ggtactggca ttgattatcc
     7441 cttcactatt gacctggcaa agcagaaagc acaatcctca ggcgggttac cgggtcaaag
     7501 gtggtcgtcc ggcgctggtg gtggtgtttc tctgtggtat tgctgtgatt ggcgtgcaat
     7561 ttttgattgc ggcagggttg ttaccagaag tggggtgatc agatagcctc aaattcctta
     7621 ttgggtgcca gaattaacgc tgacacccaa tgtgacctct taatgcaggc agcactgctt
     7681 aaatttctta ccactgccgc acggacaagg atcgttacgc cccggtttct cttctgcttt
     7741 gatcggttgc tgaacagctt tttcctgcgg atgcgccatc cagtatgcat gtaaatcaag
     7801 cgccgccagt cgaatggcat ctacgctctc ttcaaacgct tctggcgaca ttttctctac
     7861 ccgctcgaag ttttcctcag taccgtgcag cgcaatcgcc tccagcgctg gttttaaaga
     7921 atcgggcaac gttgaccagt cagaaagtgc cacgccccgc atatagccaa agcaccactc
     7981 ctcaacaatc gtcagctcgc tgccatcaac ttctcgcaag ccgaataacg gctcaaactg
     8041 ctccgggaat tcgttcagac gctctgcggt atcggccata tgttgaaaag ccaggttcat
     8101 aaagcgcgtc atctctttct ctgacgccca gcgcggcaca tagtcagccc caccccacac
     8161 ggcaaccagc cactgttctg gttcaatctc ttgcggagaa ctcaacaccg ccgtcaataa
     8221 accgtccagc tccgccacat caaggatggc gtggtcagtg ttgtatttgg tcagaatatc
     8281 gtccagccat tccaactcac tttcgtttaa cggtcccgtt ttcatacgct tttccttgtg
     8341 gatctcaact cgccagcacc tatcttacat gccggtccgt atcagagata ctttttgagt
     8401 ggctttgccg gtgattaaaa attaaggagg gtgtaacgac aagttgcagg cacaaaaaaa
     8461 ccacccgaag gtggtttcac gacactgctt attactttga ttttattctt atctttccca
     8521 tggtacccgg agcgggactt gaacccgcac agcgcgaacg ccgagggatt ttaaatccct
     8581 tgtgtctacc gattccacca tccgggctcg ggaagaaagt ggaggcgcgt tccggagtcg
     8641 aaccggacta gacggatttg caatccgcta cataaccgct ttgttaacgc gccaaattct
     8701 tcaggccttt cagccagaca tccgcttgac gccgatgtct tttaaactgg agcgggaaac
     8761 gagactcgaa ctcgagaccc cgaccttggc aaggtcgtgc tctaccaact gagctattcc
     8821 cgcattcatc aagcaatcag ttaatcactt gattttatta tcgtctggca atcagtgccg
     8881 ccgttcgatg cgctgcattc tacttacctg gcgcgatgag tcaacgatat ttttcaccac
     8941 ttttgatcgt ttgctgaaaa ttacgccgaa acgatcactg atcaagcaaa tctgcacgcg
     9001 cagcgctcaa atattgcaac attgaccaca gagtcagtac cgcagccaca aagaaaagtg
     9061 caataccggc gtactcaacc caaatgttcg gacgccacag cagccaggcc aacgccacca
     9121 tctgggcagt ggttttcact ttcccaatcc aggagacggc cacgctactg cgtttaccca
     9181 actccgccat ccattcgcgt agcgcagaaa taataatttc acgggcgatc atcgttgccg
     9241 ccggtaatgt cacccaccag ctgtgataat gctcggttac cagcaccatg gcgatagcca
     9301 cgagaacttt atctgccaca gggtcaagga aagcaccaaa ccgggtactc tggttccagc
     9361 ggcgtgccag aaaaccatcg aaccagtcag tcaccgccgc gacgcagaaa atgagcgcgg
     9421 cggcaaacgg cgaccaggtg acaggcagat aaaagaccaa tacaaagaat gggataagga
     9481 tgacacggaa cagtgtaagc aacgtaggga tattaaattg cataatgacg ggtaactatc
     9541 tgttgtcagt aagattaccc ctatgttgct acagagacat caatgtttca acgaccagaa
     9601 gatcttttct gccagacctt gcgaaatacc cggcactttt gcaatttcct cgacgctggc
     9661 gttacgtaaa ccttgcaaac cgcccatata tttcaacaac atttgccgac gttttggccc
     9721 gacgccttca atggtttcca gggaactggt atttttgacc ttcgcccgtt ttttacggtg
     9781 cccgccaatc gcgtgatcat gtgattcatc gcgaatatgc tggataacat gcagcgcggg
     9841 tgaatctggc ggcaaactaa atccctcacc ttccggctca aagaacagcg tttccagtcc
     9901 agtcttacga tctgctcctt tggcaacgcc aagtagcagc ggatgatttt tatcccatga
     9961 gacatccagt tcggcgaaga catttttcgc ctgcgcaagc tggcctttgc cgccgtcgat
    10021 aaggatcaca tccgggatct tactgtcgtc aatggcttta ccataacgcc gacgcagcac
    10081 ctgattcatc gccgcataat catcgcccgg cgtgatgcct gtaatgttat agcgccgata
    10141 ctccgcacgc agcgggccgt tagcatcaaa caccacacag gaagcgacgg tttgttcgcc
    10201 catggtatgg ctgatgtcaa agcactccat ccgcttcact tccggcaatt tcaacacgct
    10261 ggcaagcgcg gtcagtcgct ggtgaacggt agattgctgc gaaagtttgc tggttaaggc
    10321 cgtcgccgca ttggtgcgcg cgagtttcag ataacgcgcc ctatcgccgc gaggtttggt
    10381 ttgaacatta atcttgcgtc ccgccagttc tgaaagggaa tcggcgagca gcgttttatc
    10441 gctaagatta aaatcgagca ggatctcacc cggtaaggtg cgcatctggc tgccttgtaa
    10501 atagaactga cctacgaagg tttctaccac ctcgctcagt tccgtaccgc caggcacttt
    10561 cgggaaatag ctgcggctgc cgagcacttt gccctgacga atgaacaata cgtggacaca
    10621 agccatgccc gcatcgaacg ccacaccaat aacgtcaagg tcgtcgccgg tattggaaac
    10681 aaactgtttt tcggtgacgc gtcgcaccgc ctgaatttgg tcgcgaatac atgccgcttc
    10741 ttcaaactcc agattctggc tggcagtttc catacggcta atgagttgcg taagcacctg
    10801 atcatctttg ccagacaaaa acaggcgcac atactcgacc tgctgagcgt attcttcttc
    10861 actcaccagt ccttcaacgc acggtcccag acagcgccct atctggtatt gcagacacgg
    10921 acgcgagcga ttgcgataaa cactattttc gcactggcga atggggaaaa tcttttgcag
    10981 tagcgccagt gtttcacgta cggcatagcc attcgggaac gggccgaaat attcaccttt
    11041 ggaatgcttt gcaccacgat gcatcgccag acgcgggtgg gtatcgccac tcaggaagat
    11101 aaaaggataa gatttatcat cgcgtagcaa aacgttgtaa cgcggctgat agagtttgat
    11161 gtagttgtgt tccagcaaca gcgcttcggt ttctgtatgc gtaaccgtta catcaatttg
    11221 ctggatctgg gcgaccagcg cttcggtttt gcgcgaagcg aggttgctgc ggaaatagct
    11281 ggaaagccgt tttttcaggt ctttcgcttt gccgacatag ataaccgtac caccagcatc
    11341 gtacatgcga taaacgcctg gctggctggt tacggttttt aaaaacgctt ttgcgtcaaa
    11401 ctgatcactc actgacttga taatgtctcc gcattacaca gaccatggcg aattgccagg
    11461 tgagtcagct caacatcgcc atgaatgttt agtttactga acatacgata gcggtagctg
    11521 ttcaccgttt tcggactgag attgagctgt tctgagatct cattgacctt ctggccctta
    11581 gtgatcatca gcataatctg caattcacgt tcagacaaac tggcaaatgg gctttctgtt
    11641 ttttctggtt cgatctggct taacgccatt tgttgagcga tatcagaagc aatgtaacgc
    11701 tgcccagaat agacagaacg aatcgcactc acgacttcct gcggagccgc gcctttgctg
    11761 aggtagccag cagcacctgc ctgcatgact ttcgctggta aagggttttc tgtatggacc
    11821 gtaagcatga tgattttaac atcagctgtg gaacgcgcga ttttacgcgt cgcctcaaga
    11881 ccgccaatgc ccggcatact catgtccatt agcaccacgt caacagcatt tgtccggcac
    11941 cacttaacgg cgtcttcacc gcacgatgcc tctccgacga cttttatacc ctttatatct
    12001 tccagaatgc gtcgtatccc tgcgcgcacc agttcgtggt catcaacaag tagaacgttg
    12061 atcaaaggaa tatctccaga aatagggata acgctactga tagttagtca ttcgtatatt
    12121 agcggttttt attgcaactt tgaaacgtta aaaatggtgg gtattcgatt tttctctcgt
    12181 ttttgtcaat tcgattgtcc acagttatgg tcacgcccgt caccaacggc ttaccggatc
    12241 gtttcacagt aagccctacg atgaaatgtg acaaaaattg actttattca gcaaaaatga
    12301 aaatcagccc gctgaatatg taacattaat taacccgaac aatacgggca aaaaacattc
    12361 atgataaaaa tatttattgg tcattatatt aacgtatttt atagcactgc cgatatcacg
    12421 ctcaaaaaac aaccactgct atttttagca aagcttatgg tatactccgc cgccttaaca
    12481 tttttcaccg caaattttca ttgcaacatg acgaggaaaa taaatgagta cgcctgattt
    12541 ttctactgcc gagaataatc aagaactggc aaatgaagtc tcctgcctga aagcgatgct
    12601 gacgctgatg ctgcaggcga tgggacaagc tgacgcgggc cgcgtgatgt taaagatgga
    12661 aaaacagctt gcgctgatcg aagacgaaac ccaggctgca gtattttcca aaacggttaa
    12721 gcaaattaaa caagcctacc gtcagtaata taaaaccggc tgagagcgtg cctttcagcc
    12781 ggttattgac aaaatcatcg ctatctctgc tgatgtcttc ccttccgccg tccatttcag
    12841 aatttctttt tcgcgcttgc tgaaattcat ctctggcgtc atcactattt catcatttaa
    12901 acgcatcaga gccatcagac tttcgcgcac cagtaactgc atttttaatt gcagttcatc
    12961 actaagaatg ggtatttcgc gcgtgctgca acgggaaaag gacaaaaagc ccagcgcccg
    13021 atttggcagc attaaatact gagtgacacc gcggcgtaaa ccatgtgcgt gcgcggcttc
    13081 ccataatggc tgtgcttcgc tgaataagtc atcattccac attaaatggc cctgactaaa
    13141 gttttcaggg ttcagcacag gatcaattgc gagaaaattt tttgcctgat aataactaac
    13201 ccacgactca gggtaattgg tgtagaaagc cactttaggt cgcgtgaatg gtaccgggtg
    13261 gcgaacacat aacgaatagt aatcgtactc cagctgctga gcctgaagct ctatttcatg
    13321 gtagacctct tctgcggcct ccatcctctg aaaacgcaac agcatcgtgc gacgccagct
    13381 gaaaaaatcc gtatcctgca tagtaaaccg caacgcccct gagagtgagt atcatttata
    13441 atgataattc aaactatcac ataaataaga tttatatata acttatatta ttcaggcaat
    13501 gaattacttt tgcaagccat cgcattctct tatgttatta atgagttatg ct
//
LOCUS       DBKEKI010000101        13454 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.101, whole genome shotgun sequence.
ACCESSION   DBKEKI010000101 DBKEKI010000000
VERSION     DBKEKI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.101"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACUQPX_002980"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACUQPX_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HHV9057620.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="ACUQPX_002981"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="ACUQPX_002981"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HHV9057621.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="ACUQPX_002982"
     CDS             complement(3736..4092)
                     /locus_tag="ACUQPX_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HHV9057622.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSLELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="ACUQPX_002983"
     CDS             complement(4096..4512)
                     /locus_tag="ACUQPX_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HHV9057623.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACUQPX_002984"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACUQPX_002984"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HHV9057624.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5960
                     /locus_tag="ACUQPX_002985"
                     /pseudo
     CDS             5738..5960
                     /locus_tag="ACUQPX_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="ACUQPX_002986"
     CDS             complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="ACUQPX_002986"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HHV9057625.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQCVAKA
                     FAAVM"
     gene            complement(7910..8989)
                     /gene="alr"
                     /locus_tag="ACUQPX_002987"
     CDS             complement(7910..8989)
                     /gene="alr"
                     /locus_tag="ACUQPX_002987"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HHV9057626.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRGAMKYVD"
     gene            complement(9042..10457)
                     /gene="dnaB"
                     /locus_tag="ACUQPX_002988"
     CDS             complement(9042..10457)
                     /gene="dnaB"
                     /locus_tag="ACUQPX_002988"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HHV9057627.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10540..11523
                     /locus_tag="ACUQPX_002989"
     CDS             10540..11523
                     /locus_tag="ACUQPX_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HHV9057628.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="ACUQPX_002990"
     CDS             complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="ACUQPX_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HHV9057629.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="ACUQPX_002991"
     CDS             complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="ACUQPX_002991"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HHV9057630.1"
                     /translation="MHDNHETQKINQTSVMPEKTGVYWNSQFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFLGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            13191..>13454
                     /locus_tag="ACUQPX_002992"
     CDS             13191..>13454
                     /locus_tag="ACUQPX_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057631.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKA"
BASE COUNT         3400 a         3475 c         3330 g         3249 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat tccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct tgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ctcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc caggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccactc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctcttatg ccaaagattt ctttttatac atagaaacac agttaaaaat
     5881 tgcaaaagat ttttgtagac ctggagaaga agtatcaagt tctattgcaa gtaaagtttt
     5941 tcacgcattt attgatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg
     6001 cacgctttgc tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt
     6061 atttgatatt ggtaatcaat ataaggttaa acttacacaa agcggaaaaa aagagttctc
     6121 tttaacatta gaatttaacg atactataat tgaatctcag aaagtcacag gcaataaggc
     6181 aaagcatatt cttgaggata tagaaatatt ctatcgtaac aaacccgata cttattatta
     6241 aatttaataa tggttagatg tgcagaaatt attactccac acgaaaaaat agggaaagga
     6301 attccagata attgggataa acttaacaat aaaagaactg acaacgtttt attatctctc
     6361 acgtagaaca acggcatcaa aaaaatcatc tacaaagaaa tagtgaaagc gacgacagtt
     6421 taatttcact gcaggctggg taactccagc ctgctttcct gcattacatc accgcagcaa
     6481 acgcctttgc cacacattgt acatttgccg tatttaaccc ggcgacacac atgcgaccgc
     6541 tggcgatgag atagacacca aattcttcac gtagtcggtc aacctgagcg gcacttaaac
     6601 cggtataact gaacatgccg cgctgattaa gcagataatc gaaattgcgt tctggcatct
     6661 ctgtgcttaa taccttcacc aattcctgac gcattgccag aatgcgagta cgcatctctt
     6721 ctacttccgc cagccagctg gctttcaatg cctcgtcatt cagcactgca gccaccacct
     6781 gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac tgttgctttc aattgcccca
     6841 gtacgcggcc agcggcttcg gcatcttcac acagaacaga aagtccgccg acgcgctcgc
     6901 cgtaaaggga gaaaattttc gagaacgaat tgctcactag agcgggtaat ccagcgctgg
     6961 caatggcgcg aatagcgtag gcatcctctt ccataccggc actaaatcct tgataggcaa
     7021 tatcgaggaa tgggataagc tcgcgggctt tgagaatttc aatcaccgca tcccactgat
     7081 cattagtgag atcggcaccc gttgggttgt ggcaacatgg atgcagcaac acaatactgc
     7141 gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa gcgcacgctg ttagtcactt
     7201 cgtcatacca ggggtaagta ctcacttcga atccagcccc ggcgaatatt gctacgtggt
     7261 tttcccaagt aggatcgctg acccagacgc ctgattccgg gaagtagcgt ttcaggaaat
     7321 ccgcgcccac tttcaatgcc cctgagccgc caagggtttg aatggttgct acgcgctgtt
     7381 gttgcagtac cggatggtcc gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt
     7441 taagcccttc catcggtaaa taaagcgaag cgccatgggg ctgcgcattc aggcgcgctt
     7501 ccgcctccgc cacggctttc aattgtggaa taattccgtc ttcgttgtag tacagaccga
     7561 tacttaaatt cactttgtcg ctgcgagggt cttctttaaa acgctccata agcgtaagaa
     7621 tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg atggttctcc aggtttacgg
     7681 gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga tcgagtggat gttcaggagc
     7741 gaacggcaat tagcaacaga gtgagactaa tgataaacgt acatccgcca gaggcacgac
     7801 ctttataaga gcatgaaaga acatcaactt attgaatttt taggatttta ttggccggat
     7861 aaggcattca cgccgcatcc ggcacagaca atcaaatatt acagaacgat taatccacgt
     7921 atttcatcgc gccccttgaa gtcaggcgcg taataagttc gtaagcgctt actttcgtca
     7981 tttcagcgat acgttctacg ggcaaacctt cgccccataa aatgaccgga tccccggctt
     8041 tgtcctgcgc ctgtggacct aagtctacgc agatcatatc catcgccacg cgcccgacaa
     8101 tcggtacttc gcgaccgttc accagcactg gcgtaccgga cggcgcggcg cgcggataac
     8161 catcgccata gcccatcgcg actacgccaa gacgggtatc acgttcgctt acccaggttc
     8221 caccataacc aacaggctct ccggctttat gctcacgcac ggcaatcagg ctggaggtta
     8281 gtgacatcac tggctgacag ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga
     8341 cgccataaag aatgatgccc gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa
     8401 tgccacccga cgcggcaatg gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga
     8461 tagcgagttg tttctcggtt gcgccacatt ttggttcatc cgcgcgcgca aaatggctga
     8521 cgatattcac cggctgacga acgtttttgc actgggtcag gcgatgataa aacgcctcag
     8581 cctgttccgg ccttacgccc agacggtgca taccggtatc gagtttcatc cagacggtaa
     8641 ccggctcgtc caggctagcc tcttccagcg cagccagctg ttcttcgtta tgcacggcgg
     8701 tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc atcaaaaaag ccttcgagta
     8761 acagtacagg tttggtgatt ccccccgcac gcagtcgcag agcttcttcg agacgggcta
     8821 cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc aagaagaccg tgaccataag
     8881 cgttcgcttt caccaccgca accattttac tggcaggtgc cagttcacga agacgttgca
     8941 ggttgtgtcg cagagcgcgg cggttaatca caacagttgc cgcttgcatt tgtgttcctt
     9001 gataagtgtt tgctttaatt acctaattca taaaataatt attattcgtc gtcgtactgc
     9061 ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg
     9121 attgggccgt tacgttgttt accgataata atttccgcga tgccttttaa atcactgttt
     9181 tcgtgataca cctcatcacg atagataaac atgatcaagt ccgcatcctg ctcgatagag
     9241 ccagattcac gcaggtcgga gttgaccggg cgtttgtcgg cacgttgttc cagagaacgg
     9301 ttcaactggg acagcgccac caccggcacg ttcagttctt tcgccagtgc tttcagcgag
     9361 cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt
     9421 tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg
     9481 cgggaacgca cttccgttgg cgtcaggccg gaagagtcat cgatatagat attgcgtttt
     9541 tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt cttcatcatc gagctgcccg
     9601 gtacggattt tagtctggtc aacgcgcgac agcgacgcca gagaacgcat catgatctgt
     9661 tctgatggca tctccagcga gaagataagt actggtttat cctgcaacat tgccgcgttt
     9721 tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg gacgcgcggc gacgatgatc
     9781 aaatccgacg gctgcaagcc agcggttttt ttgttgagat cgtcataacc ggtgtttacc
     9841 ccggtaacgc catcgtgtgg ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg
     9901 agcacatcgg cgatgttctt cggcccttcg tctttgtttg cacgactttc ggcaatttta
     9961 aagacgcggg attcagcaag gtccagcaga tcttcgctgg tacgcccctg cggatcaaaa
    10021 ccggcttcag caatctcatt cgcaaccgag atcatctcac ggacaacggc acgttcacgc
    10081 acgatgtccg cataagcact gatgttcgcc gcacttggcg tattttttga cagctctgcc
    10141 agataagcaa aaccaccgac gctatcgagt tgcccctggc gttccaacga ttccgcaagg
    10201 gtaatcagat cgataggact accgctttcc tgcaaacgcg ccatttcagt aaagatatga
    10261 cggtgtgggc gggtgtaaaa atcgtctgct accacacgct cggctacatc atcccagcgt
    10321 tcgttatcca gcattaaacc gcccaacacc gactgctccg cttcgatcga gtgcggaggc
    10381 actttcagcc cggcaacttg tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag
    10441 ggtttatttc ctgccatagt gaatggagtt accgagataa agaatgggtc gaaactttac
    10501 catatgaagc agaccctgac gatacgttct ggaggacaca tggcaacacg aattgaattt
    10561 cacaagcacg gtggcccgga agtacttcaa gccgtagagt tcactcctgc cgatccggcg
    10621 gagaatgaaa tccaggtcga aaataaagcc atcggcatca attttatcga cacgtatatc
    10681 cgcagcggcc tttacccgcc gccatcgcta cccagcggat taggtaccga agcggcaggc
    10741 atcgtgagta aagtcggcag tggtgtaaag catattaagg ctggcgatca tgtagtctat
    10801 gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca ttaatgcgga taaagcggcg
    10861 attctgcctg cggcaatttc ttttgagcaa gctgcggcat ccttcctgaa aggcttaacg
    10921 gtttattatc tgctgcgcaa aacctatgaa attaaacccg atgaacagtt cctgttccac
    10981 gcagcggctg gcggcgttgg cttaattgcc tgccagtggg caaaagccct gggcgcgaaa
    11041 ctcatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg cgctaaaagc cggcgcgtgg
    11101 caggttatta actatcgtga agagaatctg gtcgagcgat tgaaagagat caccggcggt
    11161 aagaaagtgc gcgtagtgta cgattccgtg ggcagagaca cctgggaacg gtcgctggat
    11221 tgcctgcaac gccgcggctt aatggtcagt tttggcaact catcaggtgc ggttaccggt
    11281 gtgaacttag gcattctcaa tcaaaaaggc tcgttgtatg tgacacgccc ttccctgcaa
    11341 ggctatatca ccacgcggga ggaattaacc gaggccagta atgaactgtt ctctttgatt
    11401 gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg
    11461 cagcgtgcgc atgagattct ggaaagccgg gcgacgcaag gttccagcct gctgattcca
    11521 taaaagaaat agggcttcca cctgggaagc cctttctttt tatagttcgg ctgtatgtag
    11581 ggtacagcac gatgaatctg ttagaggcgc aatagtgaca gatttgatta tcaattccta
    11641 ttttgttcta aggataaaac cttaggttgt gatcatccgc acaatccctt agtaacgcca
    11701 gcggtcataa cgctgatatt tcggcatttt tggtgcttta atcgccttaa taacccacac
    11761 caccgcaatc gccagtagta accacggcag caacttaatc atcaatgcca gcataccgcc
    11821 gaggaacata atggccgtcg ccacaaccag cgcggcgata atgcccagca acgaaacgcc
    11881 ggtgaccatc agcatgacaa aaaagccaat cacaaaaagt agttccagca tgatgctctc
    11941 ccaaatatga aatctcttgc tggcattaca agaatcatgc caaaaataat ctattgattt
    12001 aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga atttgactac tttttggtga
    12061 aaagttaacg cttatccgcc accagtttga gcgcgtgttc cagcacgtta atgtcagcac
    12121 ccgctttatg ggcattttca cttaaataac gccgccactg ccgcgcgcca ggaataccca
    12181 ggaacaagcc cagcatatgc cggataatat ggccgagata cgtcccctgg ctgagttcat
    12241 gctcaatgta cggatacatg gcacgcacta ctgccaccgg atcggcatcg gtatccgagg
    12301 aaccaaagat ctcccggtct accgccgcca gaatacccgg attctgatac gcctcgcgcc
    12361 cgaccatcac gccatccata tgttgcaaat gcgctttagc ttcttccagc gacttgatac
    12421 caccgttaat cgacattgtc agatgcggaa agtcacgctt cagttgatac acacgcggat
    12481 aatcgagcgg cgggatttca cggttttctt tcgggcttaa ccccgaaagc caggctttac
    12541 gtgcgtggat gatgaacatc tcacactcgc ctttgccgga aacggtattg atgaaatcgc
    12601 agagaaattc atagctgtcc tggtcgtcga tgccaatacg cgttttcacc gtcaccggaa
    12661 tcgacaccac atcgcgcatc gctttcacgc agtcggcaac cagctgcgca ttacccatca
    12721 gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga
    12781 tctcatcata tccgcgcgct tctgccagct ttgcacactg cgccagcgcc gccggatcgc
    12841 taccgcccag ttgcaacgct accggatgtt cttcttcact gtacgccaga taatcacctt
    12901 taccgtgaat aatcgcccct gtggtcacca tttcggtata aagcaacgta ttgcgggaaa
    12961 gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc gagcattggt gcaacgctaa
    13021 actgagaatt ccagtaaaca ccagtttttt caggcatcac gctggtttga ttaatttttt
    13081 gtgtttcatg attatcgtgc atttttgaac atttcaggct atttttctcg cgtcaggttc
    13141 ccgcacaggt tcccacgttt tatgggaacc cgaaataacg aggtcgtgta atggcgtact
    13201 ataacataga gaaacgacta aaatccgatg gcacaccacg ctatcgctgt aatgtgatta
    13261 tcaaagaaaa aggtgttatc acttacaggg aaagcaaaac attccctaaa catgctcatg
    13321 ccaaaacatg gggcacacag aaagtgatgg aattagatct atatggaatt ccatcatcaa
    13381 atgcagttga cggacttaca gtccgtgact tactacacaa atatttaaat gacccaaatg
    13441 ccggaggtaa agca
//
LOCUS       DBKEKI010000102        13450 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.102, whole genome shotgun sequence.
ACCESSION   DBKEKI010000102 DBKEKI010000000
VERSION     DBKEKI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.102"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..189
                     /locus_tag="ACUQPX_002993"
     CDS             <1..189
                     /locus_tag="ACUQPX_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HHV9057632.1"
                     /translation="SGADLFVAWMHSMGQNLVESVAITVIGANLVDKILTAVIVWLLL
                     RQLPIRTTRHFPAMAAVR"
     gene            190..897
                     /locus_tag="ACUQPX_002994"
                     /pseudo
     CDS             190..897
                     /locus_tag="ACUQPX_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            885..1562
                     /locus_tag="ACUQPX_002995"
     CDS             885..1562
                     /locus_tag="ACUQPX_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHV9057633.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMADWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            1556..2233
                     /locus_tag="ACUQPX_002996"
     CDS             1556..2233
                     /locus_tag="ACUQPX_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHV9057634.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRMVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(2205..2918)
                     /locus_tag="ACUQPX_002997"
     CDS             complement(2205..2918)
                     /locus_tag="ACUQPX_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HHV9057635.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(2915..3424)
                     /gene="fumE"
                     /locus_tag="ACUQPX_002998"
     CDS             complement(2915..3424)
                     /gene="fumE"
                     /locus_tag="ACUQPX_002998"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HHV9057636.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(3446..4302)
                     /gene="glpX"
                     /locus_tag="ACUQPX_002999"
                     /pseudo
     CDS             complement(3446..4302)
                     /gene="glpX"
                     /locus_tag="ACUQPX_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
     gene            complement(4299..5576)
                     /locus_tag="ACUQPX_003000"
     CDS             complement(4299..5576)
                     /locus_tag="ACUQPX_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HHV9057637.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(5591..6979)
                     /gene="cmtA"
                     /locus_tag="ACUQPX_003001"
     CDS             complement(5591..6979)
                     /gene="cmtA"
                     /locus_tag="ACUQPX_003001"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HHV9057638.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITCLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLPVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(7007..7450)
                     /gene="cmtB"
                     /locus_tag="ACUQPX_003002"
     CDS             complement(7007..7450)
                     /gene="cmtB"
                     /locus_tag="ACUQPX_003002"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HHV9057639.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            7828..8736
                     /locus_tag="ACUQPX_003003"
     CDS             7828..8736
                     /locus_tag="ACUQPX_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HHV9057640.1"
                     /translation="MKAPVSLTSFHPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(8782..10773)
                     /gene="tkt"
                     /locus_tag="ACUQPX_003004"
     CDS             complement(8782..10773)
                     /gene="tkt"
                     /locus_tag="ACUQPX_003004"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HHV9057641.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            11051..11809
                     /gene="loiP"
                     /locus_tag="ACUQPX_003005"
                     /gene_synonym="yggG"
     CDS             11051..11809
                     /gene="loiP"
                     /locus_tag="ACUQPX_003005"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HHV9057642.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVAPGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(12015..12935)
                     /gene="speB"
                     /locus_tag="ACUQPX_003006"
     CDS             complement(12015..12935)
                     /gene="speB"
                     /locus_tag="ACUQPX_003006"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HHV9057643.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(13071..>13450)
                     /locus_tag="ACUQPX_003007"
     CDS             complement(13071..>13450)
                     /locus_tag="ACUQPX_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057644.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYIVN
                     AGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         3252 a         3658 c         3347 g         3193 t
ORIGIN      
        1 agcggagccg atttgttcgt tgcctggatg cactcgatgg ggcaaaacct ggtggagtcg
       61 gtggcgatta cggtgatagg cgcgaacctg gtcgataaaa ttcttacagc ggtgattgtc
      121 tggcttttac tgcgccagct gccgattcgc accacgcgcc attttccggc aatggctgcc
      181 gtgcgctaaa tgcatccatt tacctcatta acactgtggg cattggcggc ctgcaccacg
      241 cttatcctgc ccgcgcagac cattttgccg atctatagcg cggcgacctt tttctgcctt
      301 attgcgctaa aggccacgcg gcggcgggca aaatatgtcg tctggctgat gttctcgctg
      361 ggagccgggc tgtggctggt acacggtggc tggctaccag aatggctaag cggcacgcca
      421 cgctcgccag aacggtggtc gcatgctatt acgctctggt tgctgattct ggcgattgtt
      481 tcaacctcac agttgtgaat gcagtacgtc cccgtgcaat gttttattcg cgctctgttt
      541 gcttctcgcc tgccgccagg cgtcgcttat ctgtttgccg ggccgttatt ggtcgttgag
      601 cagttaaaac ggcaattagc gattattcat gaagcgcagc gtgcccgcgg cgtgccgctg
      661 gatgagggct ggtatcagcg tttacgtgct atgcccgcgt taattattcc gctaacacac
      721 aatgcgctta acgatctggc tgtccgtggt gcggcgctgg atatgcgggc atttcggatc
      781 cacaaccgac gaacaacgtt gtgggcacca gccgatagca ccctacaacg cgtagcgcgt
      841 tacaccatga tcttgctgat gctgacagaa ttcggagcgt ggagatggtt acgttagaac
      901 agttccgtta ttgcccgaca cactcaacgc atcctccgtt ctgttatgac tttcactatg
      961 tcaaaccggg gatggtcgcc atctttggtg acaacggcag tggcaaaagt acgctggcac
     1021 agctgatggc ggactggtat ccggactttc tgccaagaga gataacaggc acggggacgc
     1081 tcctcggtac gcccattggg catctcccgc ttaatgaaca atccgccacc atccagctcg
     1141 tccagcaatc gccctatttg cagctctccg gctgtacctt tagcgtggaa gaagaagttg
     1201 cctttgggcc agaaaatctg tgtcttgctg aagcagagat tatggcgcgg attgatgccg
     1261 ccctgactct gaccgagtgc cagccgttgc gccatcgtca cccggcaaca ctctccggtg
     1321 gagagacaca gcgcgtggtc atcgcctgcg ccatcgcgat gcagccaaag ctcttaattc
     1381 ttgatgaagc ctttagccgc ctgacgccgc aggccagcga aatgctgttg cagcgcttac
     1441 aacactgggc gtttgaacgt ggttcgctga ttattctttt tgaacgtcat cgcacacctt
     1501 tcctgaacta ctgccagcaa gcgtggcaat tgcaaaacgg agcgctccag ccactatgtt
     1561 aacgttaaat aagatatctt atcgctggcc tggcgcggca acggattgtc tttgcgacat
     1621 ttctctgcaa ctcaaacaag gtgaatggtt ggcgctgacc ggtgacaatg gcgcagggaa
     1681 atcgacgctg ttacgagtaa tggcaggtct tcttacgccc acagccggta cggttatgct
     1741 gcaacaacag gcgatgaaaa acctgaaaaa ccgccagcgc gcggcaaagg ttggcgtact
     1801 gtttcaggaa gcggaaaacc aactttttca cagcaccgtc gctgacgaga tcgcatttgg
     1861 tttgaaactg caaaaatgcc cggcggatga aatcactcaa cgtacccatg ctgccctgca
     1921 atgttgtcag ttagcagaca ctgccaactc gcacccgttg gatctgcata gtgcgcagcg
     1981 acggatggtc gccgtcgcct gcctggaagc actctctccg ccactattac tgctggacga
     2041 gccgagtcgg gattttgacg aaaattggtt gagcgtgttt gaaagctggc tggagaagtg
     2101 ccgccagcga ggcacgagcg tggtagcaat cagtcatgac gccgcattta cccggcgtca
     2161 tttctctcgt atggtgcgac tggaagatgg tgtaatcagg aacgttaatc caccagacga
     2221 tattcacccg tagcggtcat cattaaggtc aaatttgcag gcagactctc ttccagcacc
     2281 cggcgaacat tcggcccgtc ggtacggtca taaaaggctt ctgcatctgc cagcgatacc
     2341 cctcccgcca gcttgcgccc caccagacgc tcccgcaacg cagtagctgg cgcgttgata
     2401 aagatcgaga aatcacaaaa ttgcgcaagc tggcaccact tctcgtcgtc cagcaacaac
     2461 cagtttcctt cgacgatgac gagtggtgcg gtaacgtgca acgcatcttc aacaggatca
     2521 tgcttttgtc gatcgtactg cggccacgta caatcccctt ccaccacctg gcgcagattt
     2581 tccgccagtt tcgcaacgtc aaatgtctct ggtgcgcctt tgaaggggcg caattgatgc
     2641 gcgtccagcc agctattgta atggtgaaaa ccatccatcg ggagcgtctg aatagcgggc
     2701 agctccgggt cttgttgcgc gagatattcc cagaaggtgg ttagggtaga tttacccgtc
     2761 cccggcggag cgcaaagaaa aaccaccgta cgccgctgcg gatttaccgt ctgtaaggcc
     2821 gccagcatac gcagcaacgg tttatgcacg cgttctatct cttcatcatg gtactgcgct
     2881 tgcacgttca gcccattcac cgttaattca attttcacgg cgttaattcc tttaaggtgg
     2941 tcgttactgc cagcgtcagc gccgttttga ccattccgct aaaacgcgcc tctgaccaga
     3001 cggcgaaatc ggcgaacttc atcgctttta acgcatcgta gagcgtcgca ttgcgagtta
     3061 tactgttgac attgctgata aaatcccagg ttatatcatc ggcaaatccg ggcgtttcat
     3121 cctgttcgtt caggtaatgc cagtactggg aaaagtgcgt gatatcggca tacagccatt
     3181 tatccatttt ccccagcgca taaatcaaac gcagcgcaac atcaatatcg tcaagcgggc
     3241 cgctttgggc gagtaacggc ttcactgcat attcgacgat ctcttcatcg ttatcgacaa
     3301 acagcgacgg cagaaactgg cgtatcccct ggagcaaaat agaatgcgca gttttcataa
     3361 atgaaaataa attgtcctgt gcatccagtt gctcaagcac atcatcttct gtcagcgtcg
     3421 ccattggtct tccgatagat caccatcaat gcagggagtc tattatatta cacgtttggt
     3481 ccgcgccgcc gatcagtaat gtctgcgtcc gcaccccatt cgccatctgt tggataccgt
     3541 tcaccaactc gccgcccgtc acgcccgtag cgctaaagag gatatcgttc cccctcacca
     3601 gttcatcgag cgagtacaca cggttaacgt caacgcccat tgccttacaa cgcttgcgct
     3661 cctgctctgc aatctgccga ttttccgtgt aatccccttt agcctgacaa aagtcaatca
     3721 gctcggcctg catatccccg cctaacgctt taacggcaca ggcagaaatc acgccttctg
     3781 gcgcaccgcc gatggtgtac atcacatcgt aaggattatc ctgccagcag gtcagcacgc
     3841 tggcggcgac atcgccatcc ggcagggcaa aaaccttcac gcccagctgt gttgcttctt
     3901 caatcgcggc actcagacgc ggtttatcca gcgtaaccat gcgcagcttg tccagcggtt
     3961 tacctaacgc ccgcgccacg ttacgcaagt tgtccgccag aggcaaagac agatcaatcg
     4021 ccccggcagc cagtcgatta acaaccagct ttttcatata catatcgggc gcatgtaaca
     4081 ggctatctcg aggagcgaaa gccatgaccg ccagcgcatt gctctgcccc atcgccacca
     4141 tccgcgtgcc ttcaatggga tcaaccgcga tatcgacttc tggcgcatcg cggtgaccgc
     4201 caggccatca attttatttt tgtcgccaca tccggtttgc ggccaggcag ccaacgctgc
     4261 ctgttccgta acgcgaaata atggccacgc cagggacatc attgcgcggc ctccccgata
     4321 tcaacgccaa attgtgccag cagatatttc tcggcctgtt cattccagat gccgtgcgtt
     4381 tcctcaacca gccgcgccaa ctctttaaac agcggatcgg ttttgccttt ttcggcaaaa
     4441 tcggcaatgg cagtgagcgg catggtcacg ccgttataaa tgagtttttt accgccaggg
     4501 atatccggca gattgagcac ggtttctggc accgcatcca ggccaccaat atgggtcacc
     4561 ataaacgacg gctgtaactg tcctgtggcg ctaagggcaa tcgcctcttt catgtcgtcc
     4621 gttgaaccgc cggatgtacc gactacgtgc gtgctgttgt aatggacgtt gtagaaatta
     4681 aacggcactt tgaagttttt atccgtcggc ccggcaaaga agttcagaca gccatcttcc
     4741 gccagtaatt cgtcagccat ctcaacgaca gcaggcaccg ccgcataaac aaaaatgtca
     4801 tcgaacccga catctcctgt cagcgcccgc agcatctgga taggatcgct catccctttg
     4861 gtattcacat acaccagctc aatgccttta ctggccgcca gatccaccgg cagcagcttc
     4921 tgcacctgcg ccaggcgttt gtcgtcgata tcgaccacca ccacccgtga cggttgtatg
     4981 ccaccgttaa tggcgtaatc gatagcgcca atgcccatcg gacccgcaca cgccagcagt
     5041 gcaatattgc cgccaggttt gacgcccatg cgatgctcat aaacatattg cgtggtgtga
     5101 taattggcat gataagcacc aataatgcag cacataggct ctgccagcga cgccgcagca
     5161 aaataagagc cgtgatacgg taatacgcag cccaaattaa tggcgatttc gggaataatc
     5221 atataagtgg cattgccgcc aaaatattcg tagctgtagc ccgctgaata tccgcttggt
     5281 aaccccatcg ccggttgcaa tacaaaacgc tggccttttt tatatttgcc agtgagattt
     5341 ttacccactt cgacaataac cccggcacat tcatgcccgg taatgaccgg atgattttct
     5401 aaatcgtcgg gtacgcgttt atgttcacta ccgagtaacg ccgctttcca ggtcgataaa
     5461 cagacgctgt cagaaattac actcactaac agttcattat cggtaatttc tggcagttca
     5521 aattcgcgca gacggacatc ccgcttgcca taaatagcag caactttggt tttcattttt
     5581 acctctgtaa tcagtgttta tgttcggcgg ttaattgatt aaataaagtg tcgagttttg
     5641 gatcgccaat atagttatta atcaatatca gcggtttatc cgtcacacgt ttcacgcgcc
     5701 cttccagact ggcgtgagtg acgacgatat cggcatccgc aggcacgttt tctatcgcgt
     5761 aatgttttac ttctattgcc agccccgctt tttccaggcg tttacggaag gtggtcgcgc
     5821 ccatcgcact ggagcccatc cccgcatcgc aaacaaaggc gatacgctta accctgctta
     5881 acgagaatgc accctcttgt ttcatcgcct taaccgcatt ggctgactga gcaaactcat
     5941 cttcgctctc cgtttccacc gttttttcca tcttcagtat cagcgaagta atagcaaagg
     6001 acaccagggt acctaccgta acaccggcaa ttgtcgccag gaacgagcct ttcggcgtca
     6061 gtgccaggta cgcaaagata gaccccggac ttgggccagc caccagacca ccgtccagta
     6121 agttaaacat ccaggtacca gacataccgc ccgcaatcat ggcaataata gtcagcggct
     6181 tcatcagcac atatgggaaa tacagttcgt ggatcccacc gaggaagtga ataatcatcg
     6241 ccccgggcgc agaacgttta ctcatccctt taccaaacaa ggtaaacgcc agcagcagcc
     6301 ccaggcccgg acctgggtta gaggccacca taaagaagat ggatttaccg ttaaccgaag
     6361 cctgttgcat tcccagcgga taatagacgc cctgatcgat cgcattatta aggaaaagca
     6421 ctttcgccgg ttcattgatg actggcagca gaggcagata acccgcatgt accagcgcct
     6481 caatacactc tttgacgaaa gtattggcaa ttaacaccgc cgggccgatc acttcaaaac
     6541 ccagcagaca aaggagcatc cccgcgatac ctaatgagaa gttattgata accatctcaa
     6601 aaccggcagg tatgcgtttt tccagtgcct tatcgacata ttttatgacc aacccaccga
     6661 gcggtcccat gatcattgag ccaaggaaca tcgggatctc tgcgccaacg atcacaccaa
     6721 tagtacctat tccgcccatg accgccccgc gtttaccgcc gaccagatga ccacctgtag
     6781 aaccaatcat cacgggcaat aaacaggtaa tcatcgggcc gacaattttg gcgaaatgtt
     6841 cgttaggcag ccaaccggtg ggaataaata acgcagttat gaagccccag gcaataaaag
     6901 cacctatatt ggggatgacc attgccgtca aaaatccccc aaaagcctgg acctttgcac
     6961 gagcagactt gttttccata atattatcct gtagaggaga gagtaattat ccgcggctga
     7021 taatgtccgc taattgtttt tctgatgatg ctgttaagag ttgttcgagt atttcttctt
     7081 cgcacagtaa ttcacttaac gcctgaatag cgccaatgtg cgaatcggca tcggcagcag
     7141 agagtccgat gagtaattta attggttcgt cattccccgg gaaataaaca ccttgttcaa
     7201 gtaatgtcaa agacatcccg gttttaagcg ccccacattc cggtcgcgca tgaggcattg
     7261 ccacgcctgg tgcgagaata taataagggc cattgttaat ggtggaatct ttaattgcct
     7321 gaatgtaatt ctcgctgata tagtttttat ccagcaatga taccatcgag aaatcgatag
     7381 cttcctgcca atcttttgct gaatgtataa ctgagattga tgattctgga aaataatcac
     7441 taagccgcat aaattatcct tattttattt ttagggtacc gtccggcggt aaaaataaaa
     7501 tactcgtgcg gtttaatgaa agcaattggg tacgcgagaa gaaaaacccc ataaaccaaa
     7561 ataataaaac cttaaataac aacaatatga aattgatatt tttaaagtca actttgtatt
     7621 tgttatattc gtcacaaaat caacattttg tttgttattg tatttgtgat gcatatctct
     7681 atttatttat tgaatgttcg aaaacatact tttctaatgc aatgtttttt cgctataccc
     7741 ctttatagat tctgaattct cacctgtcgg ctgttttctc ctcatctcat gtgacatatt
     7801 cccatacttt actcaaggaa gattgtcatg aaagcacctg tttcattaac ctcatttcac
     7861 ccacaaaaat ctcttgctat cgccattggc gtgcttgccg tggttgtgct gccatttctc
     7921 tcgtattaca ccgttaatga aggtgaacgc ggcattttgc tgcgctacgg aaaaatcgta
     7981 aaagttgccg agccggggct gggttttaag atccctttta tggaaagcgt ggagaaaatt
     8041 tccacgcgta atcaggctgt ggtctatcag ggattacagg cctacagccg cgaccagcaa
     8101 ccagcacaaa tgacagtatc agtcagcttc cacattaaac cttcagaagc aggcgcggtt
     8161 tataccacct acaacactat tgaagcatta aaagatcgtc taattgtccg tcagttgcca
     8221 actcagctgg agaacatttt cggccaatat accgccatca gcgccgtaca ggatcgtaca
     8281 aaattggttc aggatctgca aaatgccatg cgcaaagccg ttgttgggcc agtggtaatt
     8341 gacggggtcc agattgaaaa tattgatttt tctgacgctt atgaaaagtc gattgaagac
     8401 cgaatgaaag cagaagtcgc cattgccacg cgtaagcaaa atctggaaac agagaaaatc
     8461 caggcgcaaa tagcggtaac tcaggcccag gcagaagcag acagtaaact ggcggcagcc
     8521 aaagcagaag cggaaactat tcgcgtaaga ggcgcggcag aggctgaaac tattcgttta
     8581 aaaagtgcgg ctgaagcaga agcaatccgc ctgcgcggtg aagctctgcg ggataatccc
     8641 ggacttgtag ccctcaccac tgccgaacgt tgggacggta aactgcccga taccatgatc
     8701 cccggcagca cagttccgtt tatctctact aagtaaaaaa agggccgctt tcgcggccct
     8761 tctcacaaaa cgtcttactg attacagcag cgcttttgct ttcgcaacaa cgttatcaac
     8821 agtgaagccg aactcttcaa acagcagctc tgccggagca gattcaccga aggtggtcat
     8881 gccgacgata gcgccgttca ggccaacata cttgtaccag tagtcagcaa tacccgcttc
     8941 taccgcaacg cgtgcagtaa ccgctttcgg cagtacggat tcacggtaag ccgcatcctg
     9001 cttgtcaaat gcgtcggtag acggcatgga caccacgcgc gctttcacgc cttcggcagt
     9061 cagtttttcg taggcagcaa ccgccagttc aacttcagaa ccggtggcga tgaagatcag
     9121 ttccggctga ccagcacagt ctttcagcac ataaccaccg cgcgcgatgt ttgccagctg
     9181 ctcttcagta cgttcctgct gcgccaggtt ctgacgggag aggataagcg cagtcgggcc
     9241 gtcctgacgc tcaacgccgt atttccacgc gaccgcggat tcaacctggt cacacggacg
     9301 ccatgtagac atgttcgggg tcacgcgcag agaagcgacc tgctctaccg gctggtgagt
     9361 cgggccatct tcgcccagac cgatggagtc gtgggtgtaa accatcacct ggcgctgttt
     9421 catcagcgca gccatacgta cggcgttacg tgcgtattcc acgaacatca ggaaggtgga
     9481 ggtgtacggc aggaagccac cgtgcaggga gataccgtta gcaatcgcgg tcataccgaa
     9541 ctcgcgaaca ccgtagtgga tgtagttacc tgcagcatct tcgttgattg ctttagaacc
     9601 agaccacagg gtcaggttag acggtgccag gtcagcagag ccgccgagga attcaggcaa
     9661 cagcgggccg aacgcttcga tagcattctg cgacgcttta cggctggcga ttttcgccgg
     9721 attagcctgc agtttagcga taaactcttt cgctttggcg tcgaagtcag acggcatttc
     9781 gcctttcata cggcgggtaa attcagccgc ttcctgcgga taagctttcg cgtaagccgc
     9841 aaacttctca ttccatgcag attctttcgc ctggcctgct tctttcgcat cccactgagc
     9901 atagatttca gacgggattt cgaacggtgc atatttccag cccagctgtt cgcgggtcag
     9961 ggcaatttca gcgtcgccca gcggcgcacc gtgggagtcg tgagtaccgg ctttgttcgg
    10021 ggaaccgaaa ccgatgatgg ttttgcacat cagcagggac ggtttgtcag tcactgcgcg
    10081 cgcttcttca actgcgcgtt tgatggatgc cgcgtcatga ccgtcgatgt cgcgaataac
    10141 gtgccagccg taagcttcga aacgcattgc tgtgtcgtcg gtgaaccagc cttcaacgtg
    10201 accatcgata gaaataccgt tgtcatcgta gaacgcaatc agtttaccca gcttcagcgt
    10261 acccgccaga gagcaaactt cgtgggagat gccttccatc atgcagccgt cgcccatgaa
    10321 ggcgtaggtg tagtggtcga caatgtcgtg gcccggacgg ttaaactgcg ccgccagcgt
    10381 tttttctgca atcgccatac cgactgcgtt ggcaataccc tgacccagcg gaccggtggt
    10441 ggtttccaca ccagcggtat aacctacttc cgggtggccc ggagttttag agtgcagctg
    10501 acggaagttt ttcagttctt ccatcggcag atcgtaaccg gtgaggtgca gcaggctgta
    10561 gatcagcatg gagccgtggc cgttggacag cacgaagcgg tcacggtcag cccaggacgg
    10621 attctgcggg ttgtgtttca ggaaatcacg ccacaggact tcggcaatgt cagccatacc
    10681 cataggggca cccgggtgac cggatttggc tttctgtact gcgtccatgc tcagcgcacg
    10741 aatagcattg gcaagctctt tacgtgagga cattttgact ccagatcgga tgatgaaggg
    10801 cacgccctta acgacttgac gacagcgcgt tttgggctac gccggaaaat ttgccaacaa
    10861 tttaccgcaa gccgcgcgtc atgtacatgg aacatccttt tgccgcttca gaaatctctg
    10921 gatcatgctc gcatgttgcg caatctgctc gcccgtccgc tgcgcttttc cttatactga
    10981 gactgagcgt cgattcacct gcaaacggcg catttttaga ataatcctga ccttgtgcgg
    11041 aagagaaaac atgaaaattc gcgccttatt ggtagcaatg agcgtggcaa cggtactgac
    11101 cggttgccag aatatggact ccaacggact gctctcatca ggagcggaag cttttcaggc
    11161 ttacagtttg agtgatgcgc aggtgaaaac cctgagcgat caggcatgtc aggagatgga
    11221 cagcaaggcg acgattgcgc cagccaatag cgaatacgct aaacgtctga caactattgc
    11281 caatgcgcta ggcaacaata tcaacggtca gccggtgaat tacaaagtgt atatggcgaa
    11341 ggatgtgaac gcctttgcaa tggctaacgg ctgtatccgc gtctatagcg ggctgatgga
    11401 tatgatgaca gataacgaag tcgaagcggt gatcggtcac gagatggggc acgtggcgtt
    11461 aggccatgtg aagaaaggga tgcaggtggc gcctggtaca aatgccgtgc gagtagctgc
    11521 ggcctctgcg ggcgggattg tcggcagttt atctcaatca caacttggtg atctgggcga
    11581 gaaattagtc aattcgcaat tctcccagcg ccaggaagca gaagccgatg attattctta
    11641 cgatcttctg cgccaacgcg gcatcagccc ggcaggtctt gccaccagct ttgaaaaact
    11701 ggcgaagctg gaagaaggcc gtcaaagctc aatgtttgac gaccatcctg catccgccga
    11761 acgtgcccag catattcgcg atcgcatcag cgcggatggg attaagtaaa gcctggtggt
    11821 gtcgaacgta ggtcagataa ggcgttcacg ccgcatctga catgaatggc acatttgtta
    11881 ccttgtgcgc attgccggat gcgatgctgg cgcatcttat ccggcctacg ggtgccgaac
    11941 gtaggtcgga taaggcgttc acgccgcatc cgacattaat ggcacgtttt acccgtgcgc
    12001 atcgcatctg gtgcttactc gccctttttc gccgcctgaa tatacagcat ctccagcgcc
    12061 agcgtcgccg cagccagagc ggtgatttcc gactgatcgt atgccggagc cacttccact
    12121 acgtccatcc caacgatgtt gagatctttc aggccgcgta ccagtttaat ggcgcgatcg
    12181 gaggtcaggc cgccaatcac tggcgtaccg gttcccggcg cgaaagcagg atccaggcag
    12241 tcgatatcaa aagtcaggta aaccggcata tcacccacaa tctgtttcac ctgggcgata
    12301 acgtcatcca cgctgcgatc gttcacctgg caggcgtcca gcacggtaaa gccgttgtct
    12361 ttatcaaact cggtacgaat accaatctgc acggaatgat tcgggtcgat cagaccttct
    12421 ttcggcgcgg tatagaacat agtgccgtgg tcaaattcac aaccgttcgc ataggtatcg
    12481 gtgtgggcgt caaagtgtac cagcgccatt ttgccgaaat gcttcgcatg agcacgcagc
    12541 agcggcagcg taacaaagtg gtcaccaccg aaggagagca tacgcttacc ggcagccagc
    12601 agcttctcgg cgtgcgcctg cagcttttcg ctcatctcac gggcatcgcc aaaggcatat
    12661 accagatcgc cgcagtccac gacgttcaga cgctcacgca tgtcgaaatt ccacgggaag
    12721 cggttgtgtt cccaggccag attcgtcgaa acctgacgga tcgctgccgg accgtggcga
    12781 ccacccgcac gaccagaagt ggccatatcg aacggcacgc cagtaatcac ccagtctgca
    12841 tcgctgtcat acggctggaa gttcatcggc aggcgtaaaa aaccaaaggc attggaaacc
    12901 agtgagttat cgtattgatg acctaaggtg ctcatggatg gactcctatt acaaagtcga
    12961 tataaaaaaa cccttccgcg tcgttaggcc cgacgaggaa gggttggatt tgcgaaacac
    13021 aagtttgtgc taattatcgc cttaaatggg cgaggcatca agcgaggttg ctatttccga
    13081 cacggtggcg gcgcatagcc attgttatct gccggaaagg cttgtccaga gcacggcggc
    13141 agcgcgtttt gcgccgggcc ttgtaattca aaggcggcaa agccgattac cccggtatta
    13201 cgcacatcgc cttgcgccga attggctgcg taggaatcag caacgttggc gaactgaaaa
    13261 gccgcttcag tatgtttatc tttgcggaat cctttaatcg ctaacgaatc tccggcattc
    13321 acaatatagc cgttgttatt cagcgaacct tgcttgccgt tcagcacatc caggccatca
    13381 accgtagcaa cgatctcgta gttggtgtta cgactgtaat tacggacata taattgataa
    13441 ttcattccga
//
LOCUS       DBKEKI010000103        13445 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.103, whole genome shotgun sequence.
ACCESSION   DBKEKI010000103 DBKEKI010000000
VERSION     DBKEKI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.103"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1689
                     /gene="ldcC"
                     /locus_tag="ACUQPX_003008"
     CDS             <1..1689
                     /gene="ldcC"
                     /locus_tag="ACUQPX_003008"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HHV9057645.1"
                     /translation="HMGGTAYQKSPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTG
                     PHLEAEEYIARTFGAEQSYIVTNGTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLM
                     MNDVVPVWLKPTRNALGILGGIPRREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGL
                     LYNTDWIKQTLDVPSIHFDSAWVPYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKML
                     AALSQASLIHIKGEYDEEAFNEAFMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINR
                     SVERALHFRKEVQRLREESDGWFFDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMF
                     LDPVKVTILTPGMDEQGNMSEEGIPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDK
                     TKAMGLLRGLTEFKRSYDLNLRIKNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHD
                     LSGLMLRAFDTLPEMIMTPHQAWQRQIKGEVETIALEQLVGRVSANMILPYPPGVPLL
                     MPGEMLTKESRTVLDFLLMLCSVGQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            1746..2135
                     /locus_tag="ACUQPX_003009"
     CDS             1746..2135
                     /locus_tag="ACUQPX_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HHV9057646.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2200..3498
                     /gene="tilS"
                     /locus_tag="ACUQPX_003010"
     CDS             2200..3498
                     /gene="tilS"
                     /locus_tag="ACUQPX_003010"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HHV9057647.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(3547..3807)
                     /gene="rof"
                     /locus_tag="ACUQPX_003011"
     CDS             complement(3547..3807)
                     /gene="rof"
                     /locus_tag="ACUQPX_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HHV9057648.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(3794..3994)
                     /locus_tag="ACUQPX_003012"
     CDS             complement(3794..3994)
                     /locus_tag="ACUQPX_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HHV9057649.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4160..4704
                     /locus_tag="ACUQPX_003013"
                     /pseudo
     CDS             4160..4704
                     /locus_tag="ACUQPX_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
     gene            4701..5123
                     /gene="arfB"
                     /locus_tag="ACUQPX_003014"
     CDS             4701..5123
                     /gene="arfB"
                     /locus_tag="ACUQPX_003014"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HHV9057650.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            5137..5847
                     /gene="nlpE"
                     /locus_tag="ACUQPX_003015"
                     /gene_synonym="cutF"
     CDS             5137..5847
                     /gene="nlpE"
                     /locus_tag="ACUQPX_003015"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HHV9057651.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6002..6826)
                     /locus_tag="ACUQPX_003016"
     CDS             complement(6002..6826)
                     /locus_tag="ACUQPX_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HHV9057652.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(6879..8597)
                     /gene="proS"
                     /locus_tag="ACUQPX_003017"
     CDS             complement(6879..8597)
                     /gene="proS"
                     /locus_tag="ACUQPX_003017"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HHV9057653.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGARVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYNKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(8708..9415)
                     /gene="tsaA"
                     /locus_tag="ACUQPX_003018"
     CDS             complement(8708..9415)
                     /gene="tsaA"
                     /locus_tag="ACUQPX_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HHV9057654.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9412..9816)
                     /gene="rcsF"
                     /locus_tag="ACUQPX_003019"
     CDS             complement(9412..9816)
                     /gene="rcsF"
                     /locus_tag="ACUQPX_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HHV9057655.1"
                     /translation="MRALPIYLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(9934..10749)
                     /gene="metQ"
                     /locus_tag="ACUQPX_003020"
     CDS             complement(9934..10749)
                     /gene="metQ"
                     /locus_tag="ACUQPX_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HHV9057656.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(10789..11442)
                     /locus_tag="ACUQPX_003021"
     CDS             complement(10789..11442)
                     /locus_tag="ACUQPX_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HHV9057657.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11435..12466)
                     /gene="metN"
                     /locus_tag="ACUQPX_003022"
     CDS             complement(11435..12466)
                     /gene="metN"
                     /locus_tag="ACUQPX_003022"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HHV9057658.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            12654..13229
                     /gene="gmhB"
                     /locus_tag="ACUQPX_003023"
     CDS             12654..13229
                     /gene="gmhB"
                     /locus_tag="ACUQPX_003023"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HHV9057659.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3266 a         3412 c         3567 g         3200 t
ORIGIN      
        1 catatgggcg gcaccgcata tcaaaaaagc ccggttggct gtctgtttta tgattttttc
       61 ggcgggaata cccttaaggc tgatgtctct atttcggtca ccgagcttgg ttcgttgctt
      121 gaccacaccg ggccacacct ggaagcggaa gagtacatcg cgcggacttt tggcgcggaa
      181 cagagttata tcgttaccaa cggaacatcg acgtcgaaca aaattgtggg tatgtacgcc
      241 gcgccatccg gcagtacgct gttgatcgac cgcaactgcc ataaatcact ggcgcatctg
      301 ttgatgatga acgatgtagt gccagtctgg ctgaaaccga cgcgtaatgc gttggggatt
      361 ctggggggga tcccgcgccg tgaatttact cgcgacagca tcgaagagaa agtcgctgcc
      421 actacgcaag cacaatggcc ggttcatgcg gtgatcacca actccaccta tgatggtttg
      481 ctctacaaca ccgactggat caaacagacg ctggatgtcc cgtcgatcca cttcgattcc
      541 gcctgggtgc cgtatactca ttttcatcca atctaccagg gtaaaagtgg tatgagcggc
      601 gagcgtgttg cgggaaaagt tttcttcgaa acgcaatcga cccacaaaat gctggcggcg
      661 ttatcgcagg cttcactgat ccacattaaa ggcgagtatg acgaagaggc gtttaacgaa
      721 gcctttatga tgcataccac cacctcgccc agttatccca ttgttgcttc ggttgagacg
      781 gcggcggcga tgctgcgtgg taatccgggc aaacggctga ttaaccgttc ggtggagcgg
      841 gcgctgcatt ttcgcaaaga ggtccagcgg ctgcgggaag agtctgacgg ttggtttttc
      901 gatatctggc aaccgccgca ggtggatgaa gccgaatgct ggcccgttgc gcctggcgaa
      961 caatggcacg gctttaacga tgcggatgcc gatcatatgt ttctcgatcc ggttaaagtc
     1021 actattttga caccggggat ggacgaacag ggcaatatga gtgaggaggg gatcccggcg
     1081 gcgctggtag caaaattcct cgacgaacgt gggatcgtag tagagaaaac cggcccttat
     1141 aacctgctgt ttctctttag tattggcatc gataaaacca aagcaatggg attattgcgt
     1201 gggttgacgg aatttaagcg ctcttacgat ctcaacctgc ggatcaaaaa tatgctgccc
     1261 gatctctatg ctgaagatcc cgatttctac cgcaatatgc gtattcagga tctggcgcaa
     1321 gggatccata agctgattcg taaacacgat ctttccggtt tgatgttgcg ggcattcgat
     1381 actttgccgg aaatgatcat gacgccacat caggcatggc agagacagat taaaggtgaa
     1441 gtagaaacca ttgcgctgga acaactggtc ggtagagtat cggcaaatat gatcctgcct
     1501 tatccaccgg gcgtaccgct gttgatgcca ggagaaatgc tgaccaaaga gagccgcacg
     1561 gtactcgatt ttctactgat gctttgttcc gtagggcaac attaccccgg ttttgaaacg
     1621 gatattcacg gcgcgaaaca ggacgaagac ggcgtttacc gcgtacgagt cctaaaaatg
     1681 gcgggataac ttgccagagc ggcttccggg cgagtaacgt gctgttaaca aataaaggag
     1741 acgttatgct gggtttaaaa caggttcacc atattgcgat tattgcgacg gattatgcgg
     1801 tgagcaaagc tttctactgc gatattcttg gtttcacgct gcaaagcgaa gtctatcgcg
     1861 aagcgcgcga ctcgtggaaa ggggatttgg cgcttaatgg gcaatatgtg attgagcttt
     1921 tctcttttcc gttcccgccg gagcgcccca gccgaccgga agcttgcggc ctgcgtcatc
     1981 tggcttttag cgttgatgac atcgatgcgg cagtggcgca ccttgaaagc cataacgtga
     2041 agtgtgaagc catccgtgtc gatccataca cgcaaaaacg cttcaccttc tttaacgatc
     2101 cggacgggct gccgttggaa ctgtatgagc agtaaggctt gtcatcgccg catttgcccg
     2161 gtaacgtgcc gggcattgct actgtaaaat cgcaccatca tgacactcac gctcaataga
     2221 caacttctca cctcacgcca gattctggtg gcctttagcg gcgggcttga ctccaccgtt
     2281 ctgctgcatc agttggtgca gtggcggacg gaaaatccgg gcgtcactct gcgcgctatt
     2341 catgtgcatc acggtttaag tgctaatgcc gatgcctggg ttacgcattg cgaaaacgtc
     2401 tgccaacagt ggcaggtgcc gctggtggtc gaacgcgtac aacttgcgca agaggggctg
     2461 gggattgagg cccaggcgcg gcaggcgcgt tatcaggcat ttgcccgtac cctattgccc
     2521 ggcgaagtgc tggtcaccgc gcaacatctc gacgatcaat gtgaaacctt tctgctggcg
     2581 ctaaaacgcg gtagcggccc tgccgggctt tcggctatgg cggaagtttc ggagtttgct
     2641 ggaacacggc ttatccgccc gttgcttgcc cgcacgcggg gggaactgga gcagtgggca
     2701 ttggcgcatg gtttacgctg gattgaagac gaaagtaatc aggacgactc atacgatcgt
     2761 aactttctgc gcctgcgcgt agtgccgtta ttgcagcagc gttggccgca ttttgcggaa
     2821 gcaacggccc gcagcgccgc actttgtgct gaacaagaga gtctgttgga tgaactgctg
     2881 gcggatgatt tagcacactg ccaaacgtcg caggggacgc tgcagattgc gccaatgctg
     2941 gcgatgagtg atgcccgtcg cgcggcgatt attcgccgct ggctggcagg gcagaatgca
     3001 ccgatgcctt cccgcgacgc gctggtgcgc atctggcagg aagtggcgct ggcgcgggaa
     3061 gatgcttccc cctgtttacg tttgggcgcc tttgaaatcc gccgctatca gtcgcaactg
     3121 tggtggatta aatccgtcac cgggcaaagc gaaaccattg tgctgtggca gacgtggcta
     3181 caaccgctgg aattaccggc ggggctggga accgtacagc ttactgcggg aggcgatatt
     3241 cgccctccgc gtgcagacga agcggtcagc gtgcgtttca aagcgccagg attgctgcat
     3301 attgtcgggc gcaacggcgg acgtaagctg aagaaaatct ggcaagagtt aggcgtgccg
     3361 ccgtggctac gtgacaccac gccactgctg ttttatggcg aaacgctgat tgcggcggca
     3421 ggggtatttg tgacgcaaga aggtgtggct gaaggtgaga atggcgtaag ttttgtctgg
     3481 cagaaaacgc ttagttaagt gaaagccgga taagacgcat caaacgtcgc atccggcgaa
     3541 agtcaatcag gattcgctta ccaccaccgt accgatttca gggtggctaa agctggtaat
     3601 tttatccaga cgcagctcgc gggtttcgcc agcggcctcg acgaccagat actccacatt
     3661 tttgcgggag actaaatcac tggcttttgc ctgcaatttt tcgccatctt tcagctcaag
     3721 tgtcagcatt aaatgatgct ggcaggcgag ctcaagatta tcgtaatcat cacaattaat
     3781 tggttgatac gtatcattca ttgacataat cgctcaccag taagtttgcc gcagcgtatg
     3841 ctgctttttc cctgacagcc tcagaaaggg cgtcgtcggc agccatttca ttcagcactt
     3901 tcaaaacgca gcccagcgcg tccggaacgt atcctaagtc tccgctggcg atttccgcgt
     3961 accgcttgcg tattaactca caatattttt ccacatgccc tcctgtcagc actctgactt
     4021 aaccgtggat gcaagtctaa gcctacgaag ataaactctg tttcgcaagg tgactatacc
     4081 acactcattt ctgcaatatc agcgccgcaa ctgcacgtat tccgttacaa tggcctcctg
     4141 attcgaaagg agttttctta tggcgcttaa agcgacaatt tataaagcga cggttaatgt
     4201 ggccgatctc gaccgcaacc agtttctcga tgcctctctg acgctggcgc gccacccttc
     4261 agaaacccag gagcgtatga tgctgcgctt gctggcgtgg ctgaaatatg ctgatgaacg
     4321 tctgcaattt acccgtggtt tgtgtgccga tgatgagccg gaagcgtggc tgcgtaacga
     4381 tcacctgggc attgatttgt ggattgagct ggggctgccg gatgagcgac gaattaagaa
     4441 agcctgcacc caggccgcag aagtggcgct tttacctata atagtcgggc ggcgcaaatc
     4501 tggtggcagc aaaatcagag caaatgtgtg cagtttgcca atctttccgt ctggtatctg
     4561 gacgatgaac aactggcgaa agtaagcgcc tttgccgatc gtaccatgac gctgcaggca
     4621 acgattcagg atggtgttat ctggttatcg gatgataaga ataatctgga agttaactta
     4681 accgcctggc aacaaccttc atgattgtga tttcccgaca tgttgctatt cccgatggtg
     4741 agcttgagat caccgccatt cgtgcgcagg gcgctggcgg gcagcatgtt aataagacct
     4801 caacggctat tcatctgcgt tttgacattc gggcgtccag cctgccagag tattacaaag
     4861 agcgtctgct cgccgccagc catcatttga tcagcagtga tggcgtgatt gtcattaagg
     4921 cacaggaata ccgcagtcag gaactgaacc gcgaagccgc tctggcccgg ctggtggcag
     4981 tgattaaaga tttaacaaaa gaacaaaaag cccgacgacc cacgcggccc acccgtgcat
     5041 cgaaagagcg caggctggca tcgaaagcac aaaaatcaag cgtgaaggcg atgcgcggca
     5101 aagtacgcag cggtcgggaa taaaaagaag gaatggatgg tgaaaaaagc gatagtgaca
     5161 gcgatggctg taatcagcct ctttactctg atgggatgta ataatcgggc tgaagtcgat
     5221 acgctttctc cggcgcaggc tgccgaactg aaaccgatgc cgcaaagttg gcgcggcgtg
     5281 ctaccgtgtg ccgattgcga aggaatcgaa atctctctgt tcctcgaaaa agacggaaca
     5341 tgggtgatga atgagcgtta tctcggtgct cgtgaagaac cttcctcctt cgcttcctac
     5401 ggtacatggg cgcgaaccgc tgacaagctg gtattaaccg acagcaaagg tgaaaagtca
     5461 tattatcggg cgaaaggcga tgcgctggag atgctcgatc gtgaaggcaa tccaattgaa
     5521 tcgcagttca actatacgct ggaaccggca caatccagtt tacctatgac gccgatgacc
     5581 ctgcgaggca tgtattttta tatggctgat gcggcgacct tcactgattg cgcgaccgga
     5641 aaacgtttca tggtagcgaa taacgcagag ctggagcgta gctacctggc tgcgcgcggt
     5701 cacagtgaaa aaccggtgtt actgtcagta gaaggtcact ttacgcttga ggctaatccg
     5761 gatactggtg cgccaaccaa agtattagcg cccgatacgg cgggtaaatt ttacccgaat
     5821 aaggattgca gtagtttggg gcagtaaacc tgcttaaggc agaaacaaac gcaaaattgc
     5881 ctgatgcgct gcgcttatca ggcctacagc atataatgca acattttgaa ttagcacgat
     5941 tttgtaggcc ggataaggcg tttacgccgc atccggcatt ttgtgcacga ggctggcttt
     6001 atcactgagt cagcccaaca aagcgacccg ccttgatata aatcccaggc ttcaggtgcc
     6061 caacatactg taactgcgtt tctggcttaa acgcagagac atcaccaata cgcatatgca
     6121 tcagatcggc agggctaatc cagcccgatt gcgccagtgt aagtggatgc ccggctttgg
     6181 caaatgcatc agtgacaaat tccgaacaaa accacgactt tttgtctcct tcgcacacac
     6241 tgcttaactg cgctttcgcc aggccgctga cgcactgttg gcgaaaatcc tcggagaacg
     6301 gattcagtga gcacatctgg cgagtcacca taaagggaat aaattcgaca atgccgcgat
     6361 agttataacc gctatctttg attttattgg caaacgcggt gatatctgtg gcttgttgcg
     6421 gggtaagatc cgggactcgt aagacgaaaa gcttatcact atgcttcatc gcttttttaa
     6481 gggaaacaat ctggacgcca gcgcctgtcg cttctgcaac gttattatca cccagaaaga
     6541 ttgcaacgtg actcacagag gaagtgctga agacgcggat tccaaatgag gttaccccaa
     6601 ggcttgagga gaacagcaaa tcgccgggtt tgagatcagg tgccgttatt tcttttattg
     6661 attgttcggt gaaagagctt tgatgctgga atttgacagc ccatgttttt gcctcggcat
     6721 ctactgctgt ggctgatggg tctggctggc tgatatcaac ggtacaagcc gaaagtaaaa
     6781 gaaagctggg gagaagcagg cggcagtacg cctttggttt atccatttta tacaatccat
     6841 gtaaaaaagg gccctgaaat tcaggaccct ttctggcatc agcctttaat ctgtttcacc
     6901 agatattcga cgatgtcacc agtcttaatt aactgtttct cgccgttgcg acgatattta
     6961 tattcgatat cgtcgttgtc gaggttacgg tcgcccagca caatagtgtg cggaataccg
     7021 atcagttcca tatcagcaaa catcacgccc ggacgctctt tgcggtcatc cagcagcact
     7081 tcgatacctt gtgcacgcag ttcgctgtac agtttctcag caagttcttg tacgcggaag
     7141 gatttgtgca tgttcatcgg cagaatcgcc acctggaacg gcgcgatagc gtcaggccat
     7201 acgataccgc gttcgtcgta gttctgctca atcgccgcag ctaccacacg cgttaccccg
     7261 ataccgtagc aacccatcgt caggatttgg ttacggccat cttcaccctg tacggaggct
     7321 ttcagtgctt cggagtactt ggtacccagc tggaagatgt gaccaacttc gataccacgt
     7381 ttgatcagca gcgtaccctg gccatccggg cttggatcgc cagccaccac gttacggata
     7441 tctgcaactt ccggggtagc gacatcgcga tcccagttga taccgaagta gtgtttacca
     7501 tcgatgttag caccagcagc gaaatcactc atcgccgcaa cggtacggtc aatcaccacc
     7561 ggaatcggca tgtttaccgg acccagtgaa cccggaccgg ctttaaccac agcacgaatt
     7621 tcttcttcgg tcgcgaaagt cagcgggctt gcaacctgcg gcagtttttc tgctttaact
     7681 tcgttcagct cgtgatcacc gcgcaccagc agcgcaacca gcgggaagct actgccttca
     7741 accgctttaa ccagcagagt cttaaccgtt ttctcaatcg gcagattgaa ctgttcaacc
     7801 agttccgcga tggttttcgc gttcggcgta tcaaccagcg tcatttcctg ggtagcagca
     7861 gcgcgcggtt ctttcggcgc gatagcttct gccagttcaa tgttcgctgc atagtcagag
     7921 gtgtcggaga agaccacatc gtcttcaccg ctctgcgcca gcacctggaa ttcgtgagag
     7981 gcgctgccgc cgatagaacc ggtgtcggcc tgtacggcgc ggaaatccag ccccatgcgg
     8041 ctgaagattt tgttgtaggc cgcatacatt gcatcgtagg tttcctgcag ggattcctga
     8101 gaagtatgga aagagtaagc atctttcatc aggaattcgc gggaacgcat gacgccgaaa
     8161 cgcggacgca cttcgtcgcg gaacttggtc tggatctgat agaagttcag cggcagctgt
     8221 ttgtaagagc taagctcgtt acgaatcagg tcagtgataa cttcttcatg agttgggccg
     8281 agtacgaacg gacgctcgcc acggtcaaca aaacgcagca gttccggacc gtactgttcc
     8341 caacgaccac tctcttgcca caaatcggct ggctgaacca ccggcatcga cacctcgatc
     8401 gcaccggcgt tgttcatctc ttcacgcacg atgttttcga cttttttcag aacgcgcgcg
     8461 ccggtcggca gccaggtata taacccggag gccagcttgc ggatcatccc ggcgcgcagc
     8521 atcagctgat ggctgatcac ctcggcgtcg gcaggtgtct ccttgagagt ggagagcagg
     8581 tattggctag tacgcatgtt gttacggttc cagttggaag gtagaacagg ctcaaggcga
     8641 gcctgggaca aaaaaagtga tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg
     8701 cgtgaattta acgcggttcc agcgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc
     8761 gaacgttaaa atcatgtagc cagacggcat aggttttgcc cgtttcctca cctttacgat
     8821 aggccgggcg cgggtcctgc gccagtactt cgcggataaa cagcgttaac tgcggataac
     8881 gcttctccag cgtcaaaagc tgcttttcga cctctgcggt aaaactcacc gccatctctg
     8941 cagctggcgc actttgcgca tagctggcac tggcatcggg aagcgattcg gcaaagggga
     9001 gatacggttt gatatccact accggcgtac catcgaccag atccagactg ccgagcttca
     9061 gaatcacgct gtctttatgg caaacaacct ctttcagctc taccagcgac atgccaattg
     9121 ggttagggcg gaaagtagag cgtgttgcga aaacccccat tctggcgtta ccgccgaggc
     9181 gcggtggacg cacagtcgga cgccagccgc cttccatcgt ttgatgaaag acgaaaagga
     9241 tccataaatg gctgaatgct tccaggccgc gaacggcgtc ggcctggttg taggaagcaa
     9301 tgagatgcag ttcgccgttg gcgcttttta ccagacctgg ctggcgcgga acggcgaatt
     9361 tttccttata gggcgagcga ataacgccta tttgctcgaa ctggaaactg ctcatttcgc
     9421 cgtaatgtta agcgcagagc cgatacatac agcctgacga tagcagcccg gcgtaccgct
     9481 ggtgacttcg cagctatgca gtaatacagc attggctttc attttagagg cattgatttg
     9541 catctgctta cgtgcggttg gaatgctcgg cggagagtcc tgattagagg cctggcaaga
     9601 gtcgccactg acttcaccga gatcgcggaa cggtttgccg actaattctt ctgcattggt
     9661 ataaattcgg accggcgtgg cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg
     9721 gggtgcagtg ctttgaacgg gttcgacagg ggatctgctt aacatggaac agccgcttag
     9781 catgagtgct actaaataga tcggtaaagc acgcatagta tttcctcaat gtatgatcaa
     9841 aacgtcaata ttgaatcagg agcttgtaaa aatgacaaga cgggcaagcg cccgtcctga
     9901 atgatattac aaattgtgga aacagcctaa aaattaccag cctttaacag cgccgccgtt
     9961 aaacactttg tttgctgctt cgtaaacttc atcagactga taagcctgga cgaatttctt
    10021 cacgttctcg gcgtctttgt tatcttcacg cgttacgatc aggtttacgt atggggagtc
    10081 tttatcttcg acaaagatac cgtctttcgc cggagtcagg ccaatctggc tggcataggt
    10141 ggtattgata actgccagag cgatttgcgc gtcgtccaga gaacgcggca gttgcggtgc
    10201 ttccagttca acaattttca gattttttgg gttctcaaca acatcaagaa cggtcggcag
    10261 caggccaacg ccatctttca gtttgatcaa gcccactttt tgcagcagca gcagtgaacg
    10321 accaagatta gttggatcgt ttggcacggc aacctgcgaa ccatcctgca gttcatccag
    10381 tgatttgatt ttcttggagt aaccagcaat cggataaaca aaagtgttgc ctactgcgac
    10441 cagtttgtag ccacgatctt tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt
    10501 ggcgtcgata tcgcctttgc tcaatgcttc gtttggcaga acatagtcgt tgaaggttac
    10561 cagctcaacg tccaggccat atttgtcttt cgcaactttc tgcgcgactt ctgctacctg
    10621 ctgttcggca ccaacaatca cgccgacttt aatgtggttt ggatcttttt catcctgacc
    10681 gcagcctacc agtgccagtg atccaatcag ggctcccact gccgcaaagg ttttgaattt
    10741 gaacgccata ccttattcct tcttcaatta tttatgttgt gttgaacgtt acttgcgagt
    10801 gacagcccgg acgatgcggt cgcctgcgaa ctgaattaaa taaaccagaa tgaccagcaa
    10861 taccagtacc gtattcatca ccgtcgcgtt gtagccgatg tagccatact gatagccaat
    10921 ctgacctaaa ccaccggcac cgactgcacc acccatcgcg gaataaccga ccagggtaat
    10981 cagggtgata gttgccgcat tcaccagacc cggcaacgct tccggtaaca gcactttacg
    11041 gacgatctgc attggcgtgg cacccattgc gcgggaagct tcaattaacc cggttgggat
    11101 ctccagcaga gcgttctcga ccatacgggc aataaacggt gctgcaccaa cggttaacgg
    11161 aacaattgct gcctgcaaac caatcgatgt accgacaata acgcgggtaa acggaatcat
    11221 ccataccagc aagataatga acgggatgga acggaaaatg ttcacaatcg cagaaacggt
    11281 acgatacagc ttcgcgttag caataatttg ccccggacgc gtgacataaa gcagaacgcc
    11341 aaccggcaga ccaatcacaa acccaaaaaa tccggatacg aaggtcattg ccagcgtttc
    11401 ccatacgcca cgaaccagca gccacatcat cggctcagac ataacccagt acctctactt
    11461 ttacatggtg ttcctgcagc caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca
    11521 tttcagtcag catgatgccg aatttaacgc caccggcgta gtccatctgc gcgctaataa
    11581 tgttgttgtt gacgttgaaa cgacgcgcgg tttcagaaag cagtggggca tcgaccgatt
    11641 gaccggtaaa ctccagacgc agcatcggca cgcagtcagt aaatggctcc gcttgcagac
    11701 gttcctggta atcttccggg atatccagat gcagggtcga ctgaataaac ttctgcgcca
    11761 gcggcgtttt cggatgcgag aacacttcac ttaccgtgtc ctgctcgatc agttcgccat
    11821 tgctgatgac cgccacgcaa tcacaaatac gcttcacaac gtccatttcg tgggtgatca
    11881 acagaatcgt caaacccaga cggcggttaa tgtctttcag cagttcgaga atagaacgtg
    11941 tcgttgccgg gtccagcgcg ctggtggctt catcacacag caatactttg ggattgctgg
    12001 ctaatgcacg ggcaatcgcc acacgttgtt tctgcccacc ggaaagattc gacgggtagc
    12061 tatcatgctt atcgccaaga ccaaccaatg acagcaattc cgtcacgcga cgtttgatct
    12121 cgtctttcgg tgtgttgtcc agctccagcg gcagagccac gttgccaaaa acagtacgcg
    12181 aagagagcag gttaaaatgc tggaaaatca taccaatctg gcggcgagct ttggtcaact
    12241 cggattctga cagcgtggtc aattcctggc catcgaccag cacgctaccc tcggttgggc
    12301 gctccagcag gtttacacaa cgtataagcg tactcttacc cgcgcctgag gcaccgataa
    12361 cgccataaat ttgtccagca ggcacatgca ggctgacgtt gttcaacgcc tggatggtgc
    12421 gggtgccctg gtggaacact ttggtgatat tcgaaagttt tatcattgat tatttattat
    12481 cgtcattaag ttagtcgtag catctcgaat gcctgaaacg ggcaacgccg tcaatgaaat
    12541 ggatgttaag gcatccagac gtctaaatca atcaggttta tgcgaagaac actttcttgc
    12601 aggtcgaaac atgcgatact agcgtcccat gccttattaa ggagctataa aaggtggcga
    12661 agagcgtacc cgcaattttt cttgaccgtg atggcaccat taatgtcgat cacggctatg
    12721 tccatgagat cgacaacttt gaatttatcg acggtgttat tgacgccatg cgcgagctaa
    12781 aaaaaatggg ctttgcgctg gtggtagtaa ccaaccagtc tggcattgct cgcggtaaat
    12841 ttaccgaagc acagtttgaa acgctgaccg agtggatgga ctggtcgctg gcggaccgag
    12901 atgtcgatct ggatggtatc tattattgcc cgcatcatcc gcagggtagt gttgaagagt
    12961 ttcgccaggt ctgcgattgc cgcaaaccac atccggggat gcttttgtca gcacgcgatt
    13021 atttgcatat tgatatggcc gcttcttata tggtgggcga taaattagaa gatatgcagg
    13081 cagcggttgc ggcgaacgtg ggaacaaaag tgctggtgcg tacgggtaaa cctattacac
    13141 ctgaagcaga aaacgcggca gattgggtgt taaatagcct ggcagacctg ccgcaagcga
    13201 taaaaaagca gcaaaaaccg gcgcaatgat taaaagatga acggttgaaa taaaaatgca
    13261 tttttccgct tgtcttcctg agccgactcc ctataatgcg cctccatcga cacggcggat
    13321 gtgaatcact tcacacaaac agccggttcg gttgaagaga aaaaatcctg aaattcaggg
    13381 ttgactctga aagaggaaag tgtaatatac gccacctcgc gacagtgagc tgagagctgc
    13441 gtcgc
//
LOCUS       DBKEKI010000104        13414 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.104, whole genome shotgun sequence.
ACCESSION   DBKEKI010000104 DBKEKI010000000
VERSION     DBKEKI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.104"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1077)
                     /locus_tag="ACUQPX_003024"
     CDS             complement(<1..1077)
                     /locus_tag="ACUQPX_003024"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9057660.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWS"
     gene            complement(1276..1360)
                     /locus_tag="ACUQPX_003025"
     tRNA            complement(1276..1360)
                     /locus_tag="ACUQPX_003025"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1324..1326),aa:Leu,seq:caa)
     gene            1556..2575
                     /gene="ahr"
                     /locus_tag="ACUQPX_003026"
     CDS             1556..2575
                     /gene="ahr"
                     /locus_tag="ACUQPX_003026"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008106 - alcohol dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HHV9057661.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2704..4206
                     /locus_tag="ACUQPX_003027"
     CDS             2704..4206
                     /locus_tag="ACUQPX_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HHV9057662.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4325..5407)
                     /gene="lptG"
                     /locus_tag="ACUQPX_003028"
     CDS             complement(4325..5407)
                     /gene="lptG"
                     /locus_tag="ACUQPX_003028"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HHV9057663.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5407..6507)
                     /gene="lptF"
                     /locus_tag="ACUQPX_003029"
     CDS             complement(5407..6507)
                     /gene="lptF"
                     /locus_tag="ACUQPX_003029"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HHV9057664.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6570..6620)
                     /gene="ytgA"
                     /locus_tag="ACUQPX_003030"
     CDS             complement(6570..6620)
                     /gene="ytgA"
                     /locus_tag="ACUQPX_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HHV9057665.1"
                     /translation="MSIIILATISTTQENK"
     gene            6774..8285
                     /gene="pepA"
                     /locus_tag="ACUQPX_003031"
     CDS             6774..8285
                     /gene="pepA"
                     /locus_tag="ACUQPX_003031"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HHV9057666.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8419..8862
                     /gene="holC"
                     /locus_tag="ACUQPX_003032"
     CDS             8419..8862
                     /gene="holC"
                     /locus_tag="ACUQPX_003032"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HHV9057667.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8862..11717
                     /gene="valS"
                     /locus_tag="ACUQPX_003033"
     CDS             8862..11717
                     /gene="valS"
                     /locus_tag="ACUQPX_003033"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HHV9057668.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENHESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11771..12967)
                     /locus_tag="ACUQPX_003034"
     CDS             complement(11771..12967)
                     /locus_tag="ACUQPX_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HHV9057669.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13160..>13414
                     /locus_tag="ACUQPX_003035"
     CDS             13160..>13414
                     /locus_tag="ACUQPX_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HHV9057670.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEA"
BASE COUNT         3462 a         3441 c         3486 g         3025 t
ORIGIN      
        1 cgaccataat cccgactcaa tcaacgaact acaagccatc gtcctgaagc catgcccaca
       61 aacctcgacc ttcgtgtcat accccattac gcgcaaagcc ttgttcaccg tattctcact
      121 catcggctta cgtggatcgt gatcgcccac aaaaatcagc tctcgatttc cgctcatgct
      181 tttgatcttt tccaaaatac ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg
      241 catcttcgag ccacgctgag aatgcttgac tccttccagt tgctcacgct cacctgggat
      301 tgtccacata gccgtttcaa aatctacttc tgaccagcgg gcaaaacgca gctcgctcga
      361 acggatgaaa accaataaag tgagttcaac cgccagtcga gttaacggtc tgccagaata
      421 gtgatctatg cgatgaagta attcaggtat acggttcagt tcaagagctg cacggtgctg
      481 tcttttcgcc gtagcaaccg cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc
      541 gctctgaacg gcaaagcgca taatcgcggt agtacgctgc tgtaaacggg cggcaacttc
      601 gagccgcccg gatgactcga ctgctttaat gggtacaaga agatcccgcg tcttcagttc
      661 cgcaatgttc cgcttaccga tagcagtaaa gagattatcc tccagacttt tcaaaacacg
      721 agcgctatgc gatgccgacc acttctgatt gctggcgtgc cagtctctgg cgactacttc
      781 aaacgttatc gcctcttgtt cctgttctac cttaacagct ttcttgtttt cactgggatc
      841 gacaccatta gctaaaagct tacgagcctc atcccggcgt gccctggcat ccgccaacga
      901 cacttcgggg tatttcccca atgccagcat cttctctttg ccgccaaagc gataacgtag
      961 ccgccagtat ttggagccgt tagggtgaac cagcaaaacc atgccttcac cgtcggtcag
     1021 cttataggct tttgcttcag gcttagccga acgaaccttc acatcactca gagccatgat
     1081 gagtatcctt tcaaggggtc tgtgtgggta caaacattat cgaaccggga aatacccgca
     1141 gttgtacccg catcagtaag ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa
     1201 aagcgaggaa agccttgcgg atactggatt ttaggcacaa aaaaagacgt ccgttgacgt
     1261 ctattgatgt tccgatggtg ccgaaggccg gactcgaacc ggcacgtatt tctacggttg
     1321 attttgaatc aactgcgtct accgatttcg ccacttcggc actgaagagg tatgcggaaa
     1381 acgttgtgga ttatacctgt cgcacgccac catgcaagcg ccagcttgca cccacctcgc
     1441 taagtgctga aattttcagc attacactca gcgatatgta acctttgtca cactccaggc
     1501 accccgccct gccatgctct acacttccca aacaacacca gagaaggacc caaaaatgtc
     1561 gatgataaaa agctatgccg caaaagaagc gggcggcgaa ctggaagttt atgagtacga
     1621 tcccggtgag ctgaagccac aagatgttga agtgcaggtg gattactgcg ggatttgtca
     1681 ttccgatctg tcgatgatcg ataacgaatg gggattttca caatatccgc tggttgccgg
     1741 gcatgaggtg attgggcgcg tggtggcact cgggagcgcc gcgcaggata aaggtttgca
     1801 ggtcggtcag cgtgtcggta ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg
     1861 tattagcggt aatcagatca actgcgagca aggtgcggtg ccgacgatta tgaatcgcgg
     1921 tggctttgcc gagaagttgc gtgcggactg gcaatgggtg attccactgc cagaaaatat
     1981 tgatatcgag tccgccgggc cgctgttatg cggcggtatc acggtcttta aaccactgtt
     2041 gatgcaccat atcactgcta ccagccgcgt tggggtaatt ggtattggcg ggctggggca
     2101 tatcgctata aaacttctgc acgcaatggg atgcgaggtg acggccttta gttctaatcc
     2161 ggcgaaagag caggaagtac tggcgatggg tgccgataaa gtggtgaata gccgcgatcc
     2221 gcaggcactg aaagccctgt cggggcagtt tgatctcatt atcaatactg tgaacgtcag
     2281 cctcgactgg cagccttatt ttgaggcgct gacctatggc ggtaatttcc atacggtcgg
     2341 tgcggttctc acgccgctgt ctgttccggc ctttacgtta attgcgggcg accgcagcat
     2401 ctctggttct gctaccggca cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg
     2461 cagcaaagtt gcgccgacaa ccgaactgtt cccgatgtcg aaaattaacg acgccattca
     2521 gcatgtacgc gatggtaaag cgcgctaccg agtagtcctg aaagccgact tctgatccct
     2581 ttgccgggtg ctgtcgcccg gcacttaaat ctctttggaa ggcgtctcgc aatctgctga
     2641 atttagcggt gaagagtgca cagccgttgc ctatactcca ggcaggaaac tggaggaaat
     2701 ctcatgagcg aacccctgtt aattgcccgc acgccggaca cagaactgtt tttactgccg
     2761 ggaatggcta accgtcacgg gctgattact ggcgcaacgg ggacgggtaa aaccgttacg
     2821 ctgcaaaaac tggcagagtc attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg
     2881 aaaggcgatc tgaccggtat cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg
     2941 cttaaaaata tcggcgtcaa tgactggcaa ccgcatgcca atccggtggt ggtgtgggat
     3001 atctttggcg agaaaggcca cacggtgcgg gcgacggttt cggatctggg gccgctgttg
     3061 ctggcgcgac tgttgaatct caacgatgtg caatctggcg tgctgaatat tatcttccgc
     3121 attgcggacg atcagggatt gttgctgctc gactttaaag atttgcgggc gattacccag
     3181 tacatcggcg ataacgccaa atccttccag aatcagtacg gaaatatcag tagcgcatcg
     3241 gttggtgcca tccagcgcgg attactgtcg ctggaacagc aaggcgcagc acacttcttt
     3301 ggtgagccga tgctggatat caaagactgg atgcgcaccg atgccaacgg taaaggcgtt
     3361 atcaatatcc tcagcgccga gaaactttat cagatgccga aactgtacgc cgccagcctg
     3421 ctgtggatgc tttcagagtt gtatgaacaa ttgccggaag cgggcgatct ggagaaacca
     3481 aaactggtgt ttttcttcga cgaggcacat ctgctgttta acgatgcacc gcaggtactg
     3541 ctggataaga ttgagcaggt gataaggctt attcgctcaa aaggcgtggg cgtctggttc
     3601 gtttcgcaaa acccgtctga tattccggat aatgtgctcg gccagctcgg taatcgtgtt
     3661 cagcacgctt tgcgggcttt tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa
     3721 accatgcggg ccaatccggc atttgatacc gaaaaggcaa tccaggaact ggggaccggc
     3781 gaggcgttaa tctcgtttct cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg
     3841 gtgatcgcac cttgttcgcg aatggggccg gtgacggaag atgagcgtaa tggcctgatt
     3901 aatcactccc cggtgtatgg caaatatgaa gatgaggtgg accgcgagtc cgcctatgag
     3961 atgctgcaaa aaggctttca ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg
     4021 aaagaggtgg cggtggatga cggtattctt ggtggattga aggatatttt gtttggcact
     4081 accggaccac gcggcgggaa gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct
     4141 cgccaggtga cgaatcagat tgtacgcggg atgttgggga gtttgctggg ggggagaaaa
     4201 agataagtca gacgtgatct gtgcccctca ccctaaccct ctccccaaag gggcgagggg
     4261 accgttcgaa ctcgtatttg actttgtcaa caataataaa gggagctttc gctcccttgt
     4321 tagattacga ttttctcatt aacagccaca ggctgattaa gaagaagctg gcacttggca
     4381 acagtgcgcc gatgatcggc gggatgccgt aaaccaacgt cagcgggccg aagatctggt
     4441 ccagtacgta gaagacaaaa ccgaagctga tacctgtgac cacacgcacg cccatcggta
     4501 cgctacgcag tgggccaaag ataaacgaca gcgccatcag catcatcacc gccacggata
     4561 gcggctggaa gattttgctc cacatgttga gctgataacg tccggcatcc tgaccgctcg
     4621 acttcagata cttcacgtag ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg
     4681 ccaccacgcc cagtttgtct ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg
     4741 aaccggtaat ctgtttcgga ttggtcagat cagattcatc aacctgcgac agacgccaga
     4801 ctttatgttc cgggtcaaac ttcgcggtag cggcataacg tacggattgc agacgacgat
     4861 tctcgttaaa ggcataaata ctgatgccac ctaactcctc gtcaccttta acccgctcaa
     4921 tgtagacgaa gttgttgcca tctttcgccc ataaaccttg ctgggtagag agcaacgagc
     4981 cgccgtacat cgcctgcgca cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc
     5041 attcgccaat cgccatcgtc agcaagacca gtggaatggc ggttttcatc accgacagcg
     5101 ctacctgcat acgggtaaaa ccagaagcct gcatcaccac cagttcgctg cgctgcgcca
     5161 gcatcccaag accaagcaac gccccaagaa gagccgccat cgggaagaag atctgcacat
     5221 ctttcggcac gctcagcaag gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc
     5281 cggctttttt cagctgatcg acaaatttga taatgcccga cagcgacacc agcatgaaca
     5341 gcgtcatcat gatggtggtg aaaatagttt taccgatata gcggtcaagt acgccaaaag
     5401 gttgcatcac accgctcctt tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt
     5461 gtcccaaagg ttgagaacaa tcgccaaagc cagataaatc aggttaacgg tccacatcca
     5521 cagtgtcggg tccagcttac ctttaccgcc gttcgatttc agggaggtct ggatcaggaa
     5581 gaaaagtaga tacagcagca tggctggcag catcgacagt acgcgtccct gacgcgggtt
     5641 aaccacgctc aacggtacaa ccataagtgc catcataaac acggtgaata ccaacgtgat
     5701 acgccagttc agttctgcgc gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc
     5761 catctggtcg gtatcgttcg ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata
     5821 atcctggaaa tccgtaatgc ggaaatcacg taacagtgca gtgccttcga agcgcgttcc
     5881 ctggttgaga gtgacgacct gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc
     5941 caccaccaca gaaggacgtg cattaccttt tggtcgaatt tgtgcgagga acacatcttt
     6001 gaaatcgctg ccgtcaacgc tttcgatgaa cagcaccgag ctgccattag tcgcttgctg
     6061 gaattgccct tgcgccagcg ccgccatgcc agggttcgct ttcgcttctg ctaacacttc
     6121 atcctgatga cgcgatgacc acggtcccgc ccacatcacg ttaaccgccg cgacgattgc
     6181 cgtgaatacc gcaaggatca ttgccgcttt caccagaacc gctttgctca agccgcaggc
     6241 atgcattacc gtaatttcac tttcggtata cagtttgccc agcgtcatca gcagcccgag
     6301 gaacaggctt aatggcagga taagctgcgc catttccggc acgcccaacc cgagaaggga
     6361 gagcaccaga ttcgccggaa tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt
     6421 ttgacagaag aagatcaaaa gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc
     6481 ccgcaccaga tatcttatga ttatcacttt aaatacgccc gtaaaaactc gtcttttgca
     6541 ggattttagc ttgtttcatg gcttaaacgt catttattct cttgagtcgt cgaaatcgtc
     6601 gctaagataa ttatactcaa cggattcacc tctcagattt tgttctgacg tgccaatgcc
     6661 gtaataacgt taagattaac acgaagtcat cgcaacagcg gacatgagtt acgaaagctt
     6721 gcaattctat ctgtagccac cgccgttgtc tttaagattc aggagcgtag tgcatggagt
     6781 ttagtgtaaa aagcggtagc ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct
     6841 tcgaaccacg tcgcctttct ccgattgcag aacagctcga taaaatcagc gatgggtaca
     6901 tcagcgccct gctacgccgg ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc
     6961 accatgttcc gaatgtactt tccgagcgaa ttctccttat tggttgcggc aaagaacgtg
     7021 agctggatga gcgtcagtac aagcaggtta ttcagaaaac cattaatacg ctgaatgata
     7081 ctggctcgat ggaagcggtc tgctttctga ctgaactgca cgttaaaggc cgtaacaact
     7141 actggaaagt gcgtcaggct gtcgagacag caaaagagac gctctacagt ttcgatcagt
     7201 tgaaaacgaa taagagcgaa ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga
     7261 cccgccgtga actgaccagc ggtgagcgcg cgatccagca cggtctggcg attgccgccg
     7321 ggataaaagc agcgaaagat ctcggtaata tgccgccgaa tatctgtaac gccgcttatc
     7381 tcgcctctca ggcgcgccag ctggctgaca gctacagcaa gaatgtcatc acccgcgtta
     7441 tcggcgaaca gcagatgaaa gagctgggaa tgcactctta tctggcggtc ggtcagggtt
     7501 cacaaaacga atcgctgatg tcggtgattg agtacaaagg caacgcgtcg gaagatgcac
     7561 gcccaatcgt gctggtgggt aaaggtttaa ccttcgactc cggcggtatc tccatcaagc
     7621 cttcagaagg catggatgag atgaagtacg atatgtgcgg cgcggcggcg gtttacggcg
     7681 tgatgcgtat ggtcgcggag ctgcaactgc cgattaacgt tattggcgtg ctggcaggct
     7741 gtgaaaacat gcctggcggg cgtgcctatc gtccgggcga tgtgttaacc accatgtccg
     7801 gtcaaaccgt tgaagtgctg aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt
     7861 taacttacgt tgaacgtttt gagccggaag cagtgattga cgtggcgacg ctgaccggtg
     7921 cctgcgtgat cgcgctgggt catcatatta ctggtctgat ggcgaaccat aatccgctgg
     7981 cccatgaact gattgccgcg tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg
     8041 gtgacgagta tcaggaacag ctggagtcca attttgccga tatggcgaac attggcggtc
     8101 gtcctggtgg ggcgattacc gcaggttgct tcctgtcacg ctttacccgt aagtataact
     8161 gggcgcacct ggatattgca ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg
     8221 gtcgtccggt agcgttgctg gcacagttcc tgttaaaccg cgctgggttt aacggcgaag
     8281 agtaagtttg cgttttaccc tcaccctaac cctctcccta agggagaggg aaccgatcga
     8341 gcgcaataga aattcattgc tcatctttca gggtgaggaa tgcagcaata tttaaatcca
     8401 ccacaagaag ccccatatat gaaaaacgca acgttctacc ttctggacaa tgacaccacc
     8461 gtcgatggct taagcgccgt tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc
     8521 agcggtaagc gcgtactcat cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa
     8581 gccctgtggg cgcgtccggc agaaagcttt gttccgcata atttagcggg agaaggaccg
     8641 cgcggcggtg cgccggtgga gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat
     8701 atattgatta gtctgcgaac aagctttgca gattttgcca ccgctttcac agaagtggta
     8761 gacttcgttc cttatgaaga ttctctgaaa caactggcgc gcgaacgcta taaagcctac
     8821 cgcgtggctg gtttcaacct gaatacggca acctggaaat aatggaaaag acatacaacc
     8881 cacaagatat cgaacagccg ctttacgagc actgggaaaa gcagggctac tttaagccta
     8941 atggcgatga aagccaggaa agtttctgca tcatgatccc gccgccgaac gtcaccggca
     9001 gtttgcatat gggtcacgcc ttccagcaaa ccatcatgga taccatgatc cgctatcagc
     9061 gcatgcaggg caaaaacacc ctgtggcagg tcggtactga ccacgccggg atcgctaccc
     9121 agatggtcgt tgagcgcaag attgccgcag aagaaggtaa aacccgtcac gactacggcc
     9181 gcgaagcttt catcgacaaa atctgggaat ggaaagcgga atctggcggc accattaccc
     9241 gtcagatgcg ccgtctcggc aactccgtcg actgggagcg tgaacgcttc accatggacg
     9301 aaggcctgtc caatgcggtg aaagaagttt tcgttcgtct gtataaagaa gacctgattt
     9361 accgtggcaa gcgcctggta aactgggacc cgaaactgcg taccgctatt tctgacctgg
     9421 aagtggaaaa ccacgaatcg aaaggttcga tgtggcacat ccgctatccg ctggctgacg
     9481 gcgcgaaaac cgcagacggt aaagattatc tggtggttgc gaccactcgc ccggaaaccc
     9541 tgctgggcga taccggtgta gctgttaacc cggaagatcc gcgttacaaa gatctgattg
     9601 gcaaatatgt cattctgccg ctggttaacc gtcgtattcc gatcgttggc gacgaacacg
     9661 ccgacatgga aaaaggcacc ggctgcgtga agatcacccc ggcgcacgac tttaacgact
     9721 atgaagtcgg taaacgtcac gccctgccga tgatcaacat cctgaccttt gacggtgata
     9781 tccgtgaaag cgcccaggtg ttcgatacca aaggtaacga atctgacgtt tattccagcg
     9841 agatcccggc agagttccag aaactcgagc gttttgctgc acgtaaagca gtcgttgctg
     9901 cggttgatgc actcggcctg ctggaagaaa ttaaaccgca cgacctgacc gttccttacg
     9961 gcgaccgtgg cggcgtggtt atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg
    10021 atgtactggc gaaaccggca gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga
    10081 agcagtacga aaacatgtac ttctcctgga tgcgcgatat tcaggactgg tgtatctctc
    10141 gtcagctgtg gtggggtcac cgtatcccgg catggtatga cgaagcgggt aatgtttatg
    10201 ttggccgcaa cgaagaagaa gtgcgcaagg aaaataacct cggtgccaac gttgccctgc
    10261 gtcaggacga agacgttctc gacacctggt tctcttctgc actgtggacc ttctctaccc
    10321 ttggctggcc ggaaaatacc gacgccctgc gtcagttcca cccaaccagc gtgatggtat
    10381 ctggtttcga catcatcttc ttctggattg cccgcatgat catgatgacc atgcacttca
    10441 tcaaagatga aaatggcaaa ccgcaggtgc cgttccacac tgtttacatg accggtctga
    10501 ttcgtgacga cgaaggtcag aagatgtcca aatccaaggg taacgttatc gacccgctgg
    10561 atatggttga cggtatttcg ctgccagaac tgctggaaaa acgtaccggc aatatgatgc
    10621 agccgcagct ggcggacaaa atccgtaagc gcaccgagaa acagttcccg aacggcattg
    10681 agccgcacgg caccgatgcc ctgcgcttca ccctggcggc gctggcgtct accggtcgtg
    10741 acatcaactg ggatatgaag cgtctggaag gctaccgtaa cttctgtaac aagctgtgga
    10801 acgccagccg ctttgtgctg atgaacacag aaggtcagga ttgcggcttc aacggcggcg
    10861 aaatgacgct atcgctggcg gaccgctgga ttctggcgga gttcaaccag actatcaaag
    10921 cgtaccgcga agcgctggac agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt
    10981 tcacctggaa ccagttctgc gactggtatc tggagctgac caagccggta atgaacggtg
    11041 gaacagaagc cgaactgcgc ggtactcgcc atacgctggt gactgtactg gaaggtctgc
    11101 tgcgcctcgc gcatccgatc attccgttca tcaccgaaac catctggcag cgtgtgaaag
    11161 tactttgcgg tatcactgcc gacaccatca tgctgcagcc gttcccgcag tatgatgcat
    11221 ctcaggttga tgaagccgca ctggccgaca ccgagtggct gaaacaggcg atcgttgctg
    11281 tacgtaacat ccgtgcagaa atgaacatcg cgccgggcaa accgctggag ctgctgctgc
    11341 gtggttgcag cgcggatgca gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc
    11401 tggcgcgtct ggaaagcatc accgtgctgc ctgccgatga caaaggtccg gtttccgtta
    11461 ccaagatcat cgacggcgca gagctgctga tcccgatggc tggcctcatc aacaaagaag
    11521 atgagctggc gcgcctggcg aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg
    11581 agaacaagct ggcaaatgaa ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag
    11641 agcgtgagaa gctggaaggt tatgcagaag cgaaagccaa gctgattgaa cagcaggctg
    11701 ttatcgccgc actgtaatca acacagtgat gaaaacgaag gccggagcat gctccggcct
    11761 tttttatctc ttacagaaat ggtaaagaag gcataactcc ccgagaaata ctggctaaga
    11821 agcctttatc tggttgttct tctttattga ttaaaggaag gtcatccaaa tcgcccagca
    11881 aatacgtatt ttttgcctgc cagtcaatca tccatatttt cagcagtggg taagccagac
    11941 cacccgtaat cccggatatc accaccaacg cacacatgcg ataaagcata ccgtggtacg
    12001 ttaaagttga gcgaaaacga atacgcccat cattcagtga caggttgctc ataaaatgat
    12061 ttcgcaacga caccgttaaa taactggtgc taacagcaat cccaaaataa taaattaact
    12121 gcgcgattat cattttacgt tgcatttcca taaattgctg taaattctca atatcatcat
    12181 ttgcataccc agaactatca tactcattta atatttgatc gaagataata taaccagcaa
    12241 cagcgagaaa aggtaataaa gctaaaaatg cgaggatggc atattttata caatacgcag
    12301 tatctaattt caccttgaaa cgatgtatac cgaaactggt attgctccag agaaaactca
    12361 ttaccaggct atataaagta ccattaaaaa taccaatgga aacaatacca actattgcca
    12421 tcagaaccac agatataata acactacttg aactattggc atgtagcatc tttgtcgaga
    12481 taaagaaaac agtccccatc ccaatggcca ttaaaatcgg caagaaaaag gttacccacc
    12541 agaacccttt caaagagcaa ttaaaactaa atcttacgcc gttgagactg gtcattaagg
    12601 cctgataacg cagtcctttt gctgccataa atataagcaa aaccaacagt aacaaagtca
    12661 aaacacagcc aacaagcggc atatctgctg acactgtcat taagattgcg aaatagaaaa
    12721 aaacaaaaac aagacaacta acaaaaacat tcccaccggt aattccataa gaaaatcgtt
    12781 gtccgttaac ttccatatta gcataaagat aacgcttaca tttcattaat gcccatggta
    12841 aatagatacc tagcgttata atcgttaaca acacattcac cacacaaata agaaaatatc
    12901 tctcacctgt accatgaaaa acaaaagaat gggacggaac atccatttca ttaataactt
    12961 gagccataaa aatcccctga attaaaaaat ccattgatat aatttataga tacagacagt
    13021 aaaatgttct gccacgttac aatactctta tcagtaatag gctggcaaaa actttattgc
    13081 tttataagac gcttgcgcac gtaagaagag agtggtatta aacaaatgta taattacttt
    13141 atttttattg ggttaaacta tgaataacat tgcgccgcaa tcacctgtaa tgcgtcgcct
    13201 gacgctgcag gataatcctg ctatcgccag cgtcatccgt caggtttacg cagagtatgg
    13261 tcttaccgcc gataaaggct acaccgttgc cgatccgaat ctcgacgaac tgtatcaagt
    13321 gtacagccag cctggccatg cttattgggt ggttgaatac aatggcgaag tggtcggcgg
    13381 cggcgggatt gcgccattaa caggtagtga agcg
//
LOCUS       DBKEKI010000105        13398 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.105, whole genome shotgun sequence.
ACCESSION   DBKEKI010000105 DBKEKI010000000
VERSION     DBKEKI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.105"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..110)
                     /locus_tag="ACUQPX_003036"
     CDS             complement(<1..110)
                     /locus_tag="ACUQPX_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057671.1"
                     /translation="MKLEDFRASTQRPFTGEEYLKSLQDGREIYIYGERV"
     gene            complement(337..1193)
                     /locus_tag="ACUQPX_003037"
                     /pseudo
     CDS             complement(337..1193)
                     /locus_tag="ACUQPX_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(1301..2191)
                     /gene="hpaA"
                     /locus_tag="ACUQPX_003038"
     CDS             complement(1301..2191)
                     /gene="hpaA"
                     /locus_tag="ACUQPX_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HHV9057672.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            complement(2201..3555)
                     /gene="hpaX"
                     /locus_tag="ACUQPX_003039"
                     /pseudo
     CDS             complement(2201..3555)
                     /gene="hpaX"
                     /locus_tag="ACUQPX_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
     gene            complement(3577..4320)
                     /locus_tag="ACUQPX_003040"
     CDS             complement(3577..4320)
                     /locus_tag="ACUQPX_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HHV9057673.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGLADLSADMGYAGNPQHPEVQAANEQLAKRYLELGALFVAVGVDTTL
                     LARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            complement(4331..5134)
                     /gene="hpaH"
                     /locus_tag="ACUQPX_003041"
     CDS             complement(4331..5134)
                     /gene="hpaH"
                     /locus_tag="ACUQPX_003041"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HHV9057674.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(5202..5582)
                     /locus_tag="ACUQPX_003042"
     CDS             complement(5202..5582)
                     /locus_tag="ACUQPX_003042"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HHV9057675.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            complement(5592..6443)
                     /gene="hpaD"
                     /locus_tag="ACUQPX_003043"
     CDS             complement(5592..6443)
                     /gene="hpaD"
                     /locus_tag="ACUQPX_003043"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HHV9057676.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            complement(6445..7911)
                     /gene="hpaE"
                     /locus_tag="ACUQPX_003044"
     CDS             complement(6445..7911)
                     /gene="hpaE"
                     /locus_tag="ACUQPX_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HHV9057677.1"
                     /translation="MKKVNHWINGKNVADNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTTTGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            complement(7908..9197)
                     /gene="hpaG"
                     /locus_tag="ACUQPX_003045"
     CDS             complement(7908..9197)
                     /gene="hpaG"
                     /locus_tag="ACUQPX_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HHV9057678.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            9469..9915
                     /gene="hpaR"
                     /locus_tag="ACUQPX_003046"
     CDS             9469..9915
                     /gene="hpaR"
                     /locus_tag="ACUQPX_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HHV9057679.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            10034..11698
                     /gene="tsr"
                     /locus_tag="ACUQPX_003047"
     CDS             10034..11698
                     /gene="tsr"
                     /locus_tag="ACUQPX_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HHV9057680.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPCQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11747..13108)
                     /locus_tag="ACUQPX_003048"
     CDS             complement(11747..13108)
                     /locus_tag="ACUQPX_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9057681.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSIRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWDLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(13323..>13398)
                     /locus_tag="ACUQPX_003049"
     CDS             complement(13323..>13398)
                     /locus_tag="ACUQPX_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057682.1"
                     /translation="LRNHLNSAKQSMIRSINENTRYAH"
BASE COUNT         3234 a         3380 c         3527 g         3257 t
ORIGIN      
        1 ttcactcgct cgccatagat atagatctcg cgaccatcct gcaggctttt cagatactct
       61 tccccggtga aaggacgttg ggtactggcg cggaaatctt ctagtttcat gtcgacctct
      121 acagtattaa tcggaatacg attgttaatt ttgtgtgttg tttttgttat ttaattgaag
      181 cgttgaacgc ggttgtcggg aagagacgtt tcaggcgaac gttggtactt ttcgacagca
      241 aggtggagtt aaatagtaaa ctgcgatcgt gatcaactta cagtaaggca aatcataata
      301 ttccccgcta acaagggcta acggggacgg aattttttat ggggcgaatg gaatggtaat
      361 cgtcatattt tcatgaccaa cgacgatagg gcctgaatag ttttctgtgc ttcttgcttc
      421 catgtttcat ccggaacggt gattggtacc aggtggctaa ggatgagttt tttcactttt
      481 gcttcattgg caattttgcc tacatcctca atagtcgtat ggtgagaagc aatggctttt
      541 gccagtgttt taccattgcc aatactgtct gccagcttag cgacggaggg ctcatgtacg
      601 gcctcatgca caagtatatc agcgccttct gagaaagttg acagtggggg aaagtacgag
      661 gtatctcctg agaagacaat tcgcttgcct tgtgtgtcga agcgataagc aaaagcctca
      721 ccgtcaggga aaggagaatg agggaccttt aatgcagtca cggtcacggt atcatcttta
      781 taaactacgc cttcaccaac atcttgagct gtaacgagtt tgcgaggatc gggttttcct
      841 tcttcttcca tacgcagtgt gatgtcccga tcgaagacct gccaactggc agcggtcatg
      901 gctcgggtgc ctggcggacc gaatacatga atagttttgt cctgcagacc gctagcccat
      961 gcattcatta agactgatgg atagtccaga atatgatcac tgtgtaaatg ggtgagtaaa
     1021 atattatcaa tctggcgcag tggaatatta ttttttacca gttgtaagct ggccccgtaa
     1081 ccagcatcaa ttaaccaggt tttattgcca ataataagtg ccgttgccat cggtaaacgg
     1141 tcagctttta ataaagaagg gccacctttc gttccaagta gtacaatttt catttattgc
     1201 ggttcggagg cgtaggtgaa tgccactggc gcaatcaggc acgccacaaa tagtgagcgt
     1261 aaagatattt tcatgtcgag tctctcaggt tgataaatgg tcagcgacca cggaattgtg
     1321 ttggtgagca acccatcatt cggttgaaaa aacgggcgaa ataggcggga tctttgtagc
     1381 caagatcaag ggctatctgg ttgatgcttt gctcgctata gagcagaagc cgtctggctt
     1441 cccggctcac tcgttcgaaa atcaatctct tcggcgattg attggcaaaa cgtcggcaca
     1501 gctcggtaag ccgtgattca ctcataccga gcattgttgc atattcaggg accattaaat
     1561 gttggcgata attttcgtca atcagccgat taaacttctg gaataaacac atctcgccgc
     1621 gcacgctgca tgttgaaata tcatttggtg gtgcttcacg cagtaggatt gtaaaaatac
     1681 tttgcgccag tgattgcaat agaatttccc gtcctggctt attttgttga aactccaggg
     1741 atattatcgg ccaataatga tttaatgccg ccagtgtatg ttccattccc tccagtgaca
     1801 gacagatacc ttgtagcatt gcggcatctc cactaccagg ccagagcttt tctaccaggg
     1861 gccaaattaa ctcctgacgt acggtcagaa catgcccatc gctatcagga tcagtaaaga
     1921 aagtatgtgg caccgatggt ggtgttagta caaaaagcgg tgcgcgcaca gaatagaagt
     1981 gctcatccag ttgtaaagta atgcgtccag tgaccagata atggagttga aaatatctat
     2041 cgtgccagtg agggcgcata tcgcgtccaa agaaattcgc taaccgggca aatgtttcat
     2101 aatgcacatc ttcaccagcg tagcgggcat catattcccg gaccaaatca atattttcat
     2161 aaccatgttc aaagatagct tcactggaca tcatgactcc ttattgcggt tttggtgcga
     2221 taagttcaga tgaaagagag tgttcccgac tccccatagg gattcgggtc atgactaacg
     2281 cgcccagtac cagcaatcct gctacaaacc acaaacctga gctaaaactg ccggttgcat
     2341 ctttcaaaat accgataagt aatggactga ctgctgaacc tacattgccg acagcattga
     2401 tgacagccac gcaacggcac gcgattgcaa gcttataact tgatccggtg ttgtccaaaa
     2461 gatagccatc gcggtaaatg atcccattga ggccataatg atgccgctca gttgaatgag
     2521 gctgtgatct gtcgcagaag cgagtaacca tccggcagcg gcaaacaggt agggaagaat
     2581 cgtatgcttt ttgcgttctt taagacggtc ggaacgacga ctccaccaga tcatgccaaa
     2641 aattgtgcag aattggggga tggccgccag caaaccaatc atgatgttgc tactgccagt
     2701 attgaaactc tgtaggattt gcggcgtcca gatattaatc gcactcagag tattggtcag
     2761 gcaaaaataa gcaagtgtat atagcatgat ggctggggtc aggacttcac gtaaggttga
     2821 gcgtggaaca gatgcatggg caaatgcact ctcttgttca cggagaatca tcgtttttaa
     2881 agcctgtttt tcgttttcat caagccaggt cgcctggtct ggtgtgtcgt tgagataaaa
     2941 ccaggtgacg acaccaagaa tgaccgatgg cagaccttcc agcaaaaaga gccattgcca
     3001 gccttttaga ttccacagtc catccaaggc cagaatgtag cctgaaagga tcaatcccag
     3061 catcatagtg actggcatcg ctatcataaa taaggcattg gctctggccc gatgatatgc
     3121 cgggaaccac caggtcagat aaaccaaaat accaggtaaa aaacccgcct cagcaatacc
     3181 aaccagcata cgcagtagat agagtgtttc ggggcttgtt gcaaacattg tgcaggtgga
     3241 agcaattccc cagaccacca tgatcccagc gatccatcga cgagcgccaa ttttcgctaa
     3301 catcatatta ctggggatcc cgcacatgac gtacgtgaca taaaagagtg tcgcggcgag
     3361 gccaaacatt gtggacgtca ggccaagatc cttgcccatt gtcagtccgg caaatcctat
     3421 attgatgcga tcaagaaacg aaaaaacaaa cagaataaaa agaaaaatga ttaggcgacg
     3481 aaataattta ttaatgacac gatgttcaat ttcaacagtt ttattatctt tatattgcag
     3541 ggtagaggtc gtcatgatgc gctccacatc gtacgtttag tagacagagt tgttgttttg
     3601 cttcgccatc gaaatagaag aactattacc aaaccgtgct gccagcgctt cggcggcgcg
     3661 ggcgagcagg gtggtgtcaa cgccgacggc gacaaacagc gcgcccagtt ccagatagcg
     3721 ttttgccagt tgctcattgg cggcctgtac ttccgggtgc tgcggattac cggcataacc
     3781 catatcggcg ctcagatccg ccaggccgat aaacacgccg tcgacccctt ccacgtccag
     3841 aatctgcggt aagttcttca ttgcctcacg cgtttcgatc tgcaccagca cgcacatttg
     3901 atcgttggct ttttgcaggt aatcaggaat gcgattccag cgcgaggcgc gagccagcgc
     3961 actgcccaca ccgcgaatac cggcgggggg ataacgggtg gcgcgtaccg ctttacgggc
     4021 ttcgtcggcg ttttgtacca tcggcaccag taaggtttgt gtgcctacgt ccagcagttg
     4081 tttgatttgc accggatcgt tccacgatgg gcgtaccact ggctggctgg ggtagggcgc
     4141 aatcgcctgt agttgggtga gcacggtttg cacgttattc ggcgcgtgct caccgtcgat
     4201 caataaccag tcgaatcctg ctccggccag taactccgcg ctgtagctgc tgctcagccc
     4261 cagccataat ccaatctgcg gacggcctgc tttcagcgcc gctttaaaac tgttttccat
     4321 cgttctctcc ttaaacaaag cggcagctaa tggagcccat gttgccgtaa tcgacgtgga
     4381 aggtgtcgcc cttacgcgcc ggaaccgggc gggtgaacga accaccgaga atgatttgcc
     4441 cggcttccag ttgtacgtca tacggtgcga gtttgttcgc cagccaggcc acgccgtttg
     4501 ccggatgatt cagcacacca gcggcgacgc cggtttcttc aatcacgcca ttgcgataca
     4561 tcagggcgga gatccaacgc agatccagtt cgtctggttt gatcggacga cctccgagga
     4621 tcactccggc gttggcggcg ttatcggaaa tggtgtcgaa tactttgcgc gggcgctggg
     4681 tttccggatc gatgttgtgg cagcgagcgt cgatcagctc cagcgccggg atcacatagt
     4741 ccgtggcgtt gtaaacgtcg aacagcgtgc agtttggccc acgcagcggt tttgccagca
     4801 caaaagccag ctccacttca atgcgcggca cgataaagcg atcggtcggg atatcgctgc
     4861 catcgtggaa gaacatgtcg tccagcagtg caccgtaatc cggttcgctg atctgcgagc
     4921 ttgcctgcat cgctttcgag gtcagaccga ttttgtggcc tttcagcgtg cgaccttcgg
     4981 cgattttcag gcgaacccat tcgcgctgca cggcgtaggc gtcttcgatg gtgatctccg
     5041 ggtaatccag cgagatcgcg cggatctgtt cgcgctgttt ttctgcctga tccagacgct
     5101 gggcgatcag ggtgtgggtg tgtttatcga acatagcgat atcctgtggc attgccggat
     5161 gcggcagcgc cttatcatcc ggcctggaag ctggtgcaaa tttatttaaa caacgcgtgt
     5221 acgttgttct gtttgaaatt caacgtcggg tgcagctctg caatctcaaa tgaaagcgcc
     5281 aggtaacggc tctccatcag tgaggcgaag tgcgttttaa tcagatcaaa cagcatttcg
     5341 cccacttcct gacggctttc cagactgcgt ccgctgccga tttttagcgt catatgcaca
     5401 aaggcgtaat cgtgcttgcc atcagccatt tgccaggtat cgatccagtg cgcccggcta
     5461 cgtattcccc caagcggaaa aatccccgtt gccgccagtg cagtgttaac actggcaaac
     5521 agctcaggta agcgggcttc ttcacgaata ttttcagtac attccacgat aaagtgcggc
     5581 ataaaacgtc cttacgcggg cagcgggaaa acagcgttaa cctgaccggt gccagagctt
     5641 gggaacagct cggtaataaa ctccaccttg ccgtcgtatt tatcccagcc gagcatcccc
     5701 agcagcatca ccgtgtcgtg catattgcct tcgccgtagc agtagtcggc gtactccggc
     5761 agcatattgc aaaactcttt gaactggcct tcgcgccaca gcttcaccac acgctcgtcc
     5821 atctggcggt cgaactcgcg ggtgtagctg ttcatccctt cttccgcacg ctgatcgtca
     5881 ataaagcggt gcgataacga accgctggca aggaccgcca cggtgccgtc gtactgttcg
     5941 atcgctttca gaatcgcttc gcccagcttg cggctgtcgg caaaatcgtg aaccgtgcag
     6001 aaagccgaaa tggagaccac tttgaagtgc ttgtcttcat tcatgtagcg catcggtacc
     6061 agcgtgccgt attccagttt caggctggga atattgtgcg cttttgcccg cacgccgagc
     6121 tttaaggctt cctcggcaat tagctgcccc aactccgggt tgccctcgta gttgtaggtc
     6181 atgtcgcgaa taaaatgcgg cagctcgttg ctggtgtaga cgccttcaaa atggtctgca
     6241 cagttgatgt gataagcact gttgaccagc cagtgagtat cgaaaacgat aatggtatcg
     6301 acgcccattt cccggcaacg cttgctgatc tctttatgcc cgtcgatcgc accctggcga
     6361 caaccgtggt tttttcctgg tagttcagag agatacatcg acggtacgtg cgtgatcttg
     6421 gcggctaacg ctaacttacc catatcagac tccccatttc ggaattggat ggtcgcccat
     6481 ggaaatgcag acgttcttca tttccgcgaa cacttcgaag ctgtactcac cgccttcacg
     6541 cccggtgccg gaggctttta cgccgccaaa tggctggcgc aggtcgcgca cgttctgggt
     6601 gttgacgaac accatgcctg cttcaatgcc acgcgccagg cgtaacactt tgctgacatc
     6661 ctgtgtccag atgtacgacg cgaggccgta ctccacgtcg tttgccaggc gtaagccttc
     6721 ggcttcgtct ttaaacggca gcaggcaggc gaccggcccg aaaatctctt cctgggcaac
     6781 gcgcatacgg ttatctacgt ccgccagcac cgttgggcgc aggaagttgc cgcctttcag
     6841 gtgcgcaggc aggtcggacg gtttatccgg gccgcccgcc aacagggttg cgccttcttc
     6901 aatgccgaga cggatatagc cggagacttt ttcccagtgt tgctgactga taagcgccct
     6961 aacctgggta ttcggatcgt tcggatcgcc cacgcgcaga cggttggcgc gttcggcaaa
     7021 gcgtttaacg aattctggat agatgctctg ttgaataaag atgcgcgaac cggcggtgca
     7081 gcgttcgccg ttgattgaga agatggtgaa cagggcggcg tccagcgcgc gctcaatatc
     7141 ggcatcttca aaaatcagca ccggtgattt accgcccagc tccatggagt attttttcag
     7201 cccggcgttt ttcatgatgt tgcgcccggt ggtggtaccg ccggtgaacg acacggcacg
     7261 cacgtcatga tgacggacca gcgcatcgcc tgcggttgcg ccgtagccct gtactacgtt
     7321 tagcacgccc gccggaatac cagcttccag cgccagctca cccaggcggt tggcggtcag
     7381 cggggagagt tccgacatct tcagcaccgc ggtattgccc agcgccagac acggcgcgac
     7441 cttccaggtg gcggtcataa acggcacgtt ccacggcgac accagcgcac aaacgcctac
     7501 cggttgcacc agcgtgtagt tgagcatctt gtcgtcaacc ggataggtct tgccgttcat
     7561 ctgctggcag acttccgcga aaaattcaaa gttgtgagaa gcgcgtggga tcaacacatt
     7621 tttggtctga tggatcggca ggcccgtgtc cgcggtttcc atcgcggcga tctcaggcac
     7681 gttctggtcg atcagatcgc ccagacggcg catcaggcgc gcacgctctt tcatcggcag
     7741 atcagcccat ttcgggaacg cctctttcgc tgccgctacc gcctgattga tctccgcttc
     7801 accgccagag gccacatccg ccagcacttc acccgttgcc ggattggtag tctggaagta
     7861 gtcgttatct gcaacatttt tgccgttgat ccaatgattt acttttttca tttcgctgtt
     7921 tcctcactca caattcggtt caccaggcgg cccacgcctt ctacttccac cactacttca
     7981 tcgccaggca ccacgtcaga taagcctttt ggtgtgccgg tggcgatcat gtcgcccgga
     8041 ttcagggtca taaattcgct taagtaggcg atcaggaagg gcacgctgaa gatcagatcg
     8101 gcggtggtgc cttgctggcg taactcgccg ttgacgaagg tgcgaagggt cagattatgc
     8161 gggtccggga tcgcctcttt cggcacgatg gttgaaagca tcggcgtcag tccgtcgcgg
     8221 cttttgaccc gcaggttagg gcggtagtag ttttccagat agtcgcgaat ggcgtagtcg
     8281 ttacacacgg tgtagcccgc gacataatcc atggcatcgg cttcgctgac gttacgcgcc
     8341 tgcttgccaa taactaccac cagctccgct tcgtagtgca tgtattcaat attgttcggg
     8401 cgcacggagg tctggttgtc gccagtgagg gtattcggcg ctttcaggaa caccagcggc
     8461 tcttccggtg gcttaaattc cagttcgctg gcgtggtcgg cgtagttcag gccgagggca
     8521 aacagcgtac cgtgcgggtg tggcagcgtt gggaagctct tgcgcgtggt cacttcacgt
     8581 tcgtccacta ccggattttc cagcggcggg aaaccttctg cgagaacacg cacgcgatcg
     8641 cctggctgta tttccacgcg cgcctgtggc gtgccgagca gaatagcatc gcctggattc
     8701 agcgtggcaa attcgctcag ggcgctcagc agctgtgcgg cgttacgttg taaatcggcg
     8761 gtattccagt gatcggcagg acgcccgttg atctcggtat agatagtcag attatcgaca
     8821 ttgctgagag ccacggtttc gccaatgggg cagaatccat cacgacattt tgctttgatt
     8881 gccggacggt aaaagctctc ttccggcagg ctgacgtcgt tagccagcgc atatccggcg
     8941 atgtactctg ccgcatcttc ttcacgtact ttcgtcgccg ttttccccac aatcagagca
     9001 acagtcgcac cgctcagtac tttttcaccc tgtggaaagg gaatcggttc accgcaacca
     9061 atcaccgtat tgcgcggttt aataaaccag accgcagttt taggcggggc tttgtaggga
     9121 gattgctgga acgcttcctg ccaggcatca agttggctgc gatggttcaa cgctacggcg
     9181 aagatagtgc ctttcattac cactcctcgg attcgatcat cttaatcatt aatatgttaa
     9241 tgattacttt tattcccgat tgcgtagttt ggcaaaagta agtgatataa atgtgatcta
     9301 attaacaaac tatttacatt taaagataat aaaatattaa atatcagtaa gttaataaaa
     9361 tatcatcaat tttgataaaa tgaagaattg cgggggcagc tcagtcccgg taacgtgatg
     9421 attaattgtt tctatattaa tgattttgaa tttttcaggg gaaagataat gcacgactca
     9481 ctaaccattg cattgctcca ggcgcgagaa gcggcgatga gttattttcg cccaatcgtt
     9541 aaacggcata atttgactga gcaacagtgg cgcatcgtgc gtattctggc ggaaagccca
     9601 tcaatggatt ttcacgatct ggcgtatcgc gcctgcattt tgcgcccaag cctgaccggg
     9661 atcctcacgc ggatggaacg cgacggttta gtgttgcgat taaagccgat caatgatcag
     9721 cggaagctct atatctcgct gaccaaagag gggcaggcgc tgtataaccg cgctcagaca
     9781 cagattgaag aagcctatcg gcagattgaa gctcagttta ctgccgagaa aatgcaacag
     9841 ttaacccatc tgttagagga atttattgct ctgggcaatt cccgacaaga agacatcccg
     9901 ggcgataatg aataactttt tagtatctga taaccagcgc ctggtttttc catggatggc
     9961 gggttacatc ttttcataaa gtttttgctt tccaggccga aaatcttgca tcggtccaca
    10021 ggaaagagaa accatgttaa aacgtatcaa gatcgtgacc agcttactgc tggttttggc
    10081 cgtttttggc cttttacaac tgacatcagg cggtctgttt tttaatgcct taaagaatga
    10141 caaagaaaat ttcactgttt tacaaaccat tcgccagcag caatccacgc tgaatggcag
    10201 ctgggtcgcg ttgttgcaga cgcgtaacac cctcaaccgc gcgggtatcc gctacatgat
    10261 ggatcagaat aatattggta gcggttcaac cgttgctgag ctgatgcaga gtgccagtat
    10321 ttcgctgaaa caggcggaaa aaaactgggc ggattacgaa gcgttgccgc gtgacccgtg
    10381 tcagagcacc gccgcagcgg cagagatcaa acgtaattac gatatttatc acaatgcgct
    10441 ggcggagctg atccaactgt taggtgcagg caaaatcaac gagttctttg atcagccgac
    10501 ccagggatat caggacggtt tcgagaagca gtatgtggct tacatggagc aaaacgatcg
    10561 gctctatgat atcgccgtca gcgataacaa tgcctcctac agccaggcga tgtggattct
    10621 ggtgggcgtg atgatcgtcg tactggcggt catcttcgcc gtctggttcg gtattaaagc
    10681 ctcgctggta gcgccaatga atcgcctgat tgacagcatt cgtcatattg caggcggcga
    10741 tctggtgaaa ccgattgagg tggatggctc taatgagatg gggcaactgg cagagagttt
    10801 gcgccatatg cagggagagc tgatgcgtac cgtcggtgat gtgcgcaacg gggccaatgc
    10861 catctatagc ggtgccagcg aaatcgctac cggcaataat gatctctctt cgcgcaccga
    10921 gcaacaggcc gcttcgctgg aagagacggc agccagcatg gagcaactga ccgcaacggt
    10981 gaaacagaac gccgagaatg cgcgccaggc cagccatctg gcattaagtg cttctgaaac
    11041 ggcgcaacgc ggcggcaaag tggtagataa cgtggtgcag actatgcgcg atatctccac
    11101 cagttcgcag aaaatcgccg atattatcag cgtaattgac ggcattacct tccagaccaa
    11161 tattctggct ttgaacgcgg cggttgaagc agcgcgtgcg ggtgagcaag gacgtggttt
    11221 tgcggtggtt gcgggagaag tgcgtaatct ggcccagcgt agcgcccagg cggctcgtga
    11281 aattaaaagc ctgattgaag actcggtggg gaaagtggat gttggctcta cgctggtcga
    11341 aagcgccggg gaaacaatgg cggagattgt cagtgctgtg acccgcgtga cggacattat
    11401 gggcgaaata gcttctgctt ctgatgagca gagccgtggt atcgatcagg ttggcttagc
    11461 ggttgctgag atggaccggg taactcaaca gaacgccgcg ctggtggaag aatctgccgc
    11521 tgccgccgcc gcgctggaag agcaggccag tcgcctgacc gaagctgtgg cagtgttccg
    11581 gattcagcaa cagcagcaac agcagcgtga aacatcggct gtggtaaaaa ccgtgacgcc
    11641 agctacgccg cgtaaaatgg cagtggcaga tagcggggag aactgggaaa cgttttaatc
    11701 gccataaaaa tgcccgataa gcaaaatgtt atcgggcata gggagtttaa tctttacgcg
    11761 ggtcgttgat cggctggcga accaggaaga tgtacgccag tgctcccgct gcggtgacgc
    11821 aaccgcaaat gattagtgcc agacggaatg agtgggttgt atcaacaata aaaccagtaa
    11881 tgatcggcgc gaaagaggcg cagatgaagc tggcaaagtt ctggatactg cccaccgacg
    11941 cagtcatgcg agaagcaact gcgacgtgga tcaagtccca gcaggatgtt ccggcaaagt
    12001 gaatacagaa cagtgccatg ccaatcagca gaaccgccat catggatgtt gtcgcttgtg
    12061 gtactatcag cgtaaaggcg gcagaacaga acatcccggt aataatgcag atcttacggc
    12121 ttttaatcgg agccattccc cctttgacca gccagtcagt aacgtaaccg ttgaccagca
    12181 tcccggcagc cccaaacagg aaagggatag ccgccatcaa ccctgtgctt tttaaatcca
    12241 ggttataggc tgtttgcagg taaccaggaa gccaggccag atacagccac gcagtgtagt
    12301 tgatgccact gaatccgagc atcattcccc acattgtgcg gttacggaac aggctgcgcc
    12361 attcggcaaa attgagcgga tctcggcggg cattgacgct acctgcattg agataagctt
    12421 gttcaacggc agtcagttct acgtgctcgc ggttgcgata gagcatatac cagccgatgg
    12481 cgagaaaaat ccccagtaca ccaatggtaa taaacatccc gcgccagccc atcaccagca
    12541 tcattgccgc cagaatcggt gggcttacag caacgccaat ggtagaagct gcgttgaaga
    12601 agcccatcgg gcgtccgcgc tctttgatgt tgaaccagtc gttaatgact tttacaccgc
    12661 atgggttcat cggcgcttca ccaatcccca taccgatacg caccaacacg aactgcgtaa
    12721 agctgtgcac catgccagac atggcctgga acagtgacca gaagaacatc cccagtccca
    12781 gcatcaggcg tgggccttta cgatccaata gtgggccgca aggaagttgc gcaatgccgt
    12841 aagcgagtga aaacacggag agcaaagcgc cgatttcggt ggcacttaat cccaattctt
    12901 cacgaatcgt taaatttgct accgacagcg aactgcggtc gagataattg attaccgccg
    12961 caaaaaataa taataacatg gcggtggttt gaatgcgttt aattcggata ctgcgttgaa
    13021 ctaatccatc tggtggcacg ggaatatctg ccgacgaaga tggagtaaat gaagaacgcg
    13081 gatcgagggt gatattttct ttttccacgc cggactcctg tatttataat tttaattgtt
    13141 tacgtaattg tgcattacgc aaggttgaca ttaacggcag gttaaagttt taggacttta
    13201 ctaccgctca caaaaacatt tttagtcgac tgattgtgag cataggtaag cgggaatttt
    13261 ccggtattta tcttttgata aaaccgtgca cccgctaata gtttgctgtt tttaatccgt
    13321 acttaatgag cataacgcgt gttctcatta atggatcgaa tcattgattg tttagctgaa
    13381 ttcaaatggt tacggagc
//
LOCUS       DBKEKI010000106        13388 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.106, whole genome shotgun sequence.
ACCESSION   DBKEKI010000106 DBKEKI010000000
VERSION     DBKEKI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.106"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(9..461)
                     /locus_tag="ACUQPX_003050"
     CDS             complement(9..461)
                     /locus_tag="ACUQPX_003050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHV9057683.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(471..869)
                     /gene="yafO"
                     /locus_tag="ACUQPX_003051"
     CDS             complement(471..869)
                     /gene="yafO"
                     /locus_tag="ACUQPX_003051"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HHV9057684.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(872..1165)
                     /gene="yafN"
                     /locus_tag="ACUQPX_003052"
     CDS             complement(872..1165)
                     /gene="yafN"
                     /locus_tag="ACUQPX_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HHV9057685.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(1217..2272)
                     /gene="dinB"
                     /locus_tag="ACUQPX_003053"
     CDS             complement(1217..2272)
                     /gene="dinB"
                     /locus_tag="ACUQPX_003053"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HHV9057686.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCTHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2343..3128)
                     /gene="lafU"
                     /locus_tag="ACUQPX_003054"
     CDS             complement(2343..3128)
                     /gene="lafU"
                     /locus_tag="ACUQPX_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HHV9057687.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQRLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            3094..4812
                     /locus_tag="ACUQPX_003055"
                     /pseudo
     CDS             3094..4812
                     /locus_tag="ACUQPX_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(5029..5526)
                     /gene="rayT"
                     /locus_tag="ACUQPX_003056"
     CDS             complement(5029..5526)
                     /gene="rayT"
                     /locus_tag="ACUQPX_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HHV9057688.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(5702..6229)
                     /locus_tag="ACUQPX_003057"
                     /pseudo
     CDS             complement(5702..6229)
                     /locus_tag="ACUQPX_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            6607..7347
                     /gene="dpaA"
                     /locus_tag="ACUQPX_003058"
     CDS             6607..7347
                     /gene="dpaA"
                     /locus_tag="ACUQPX_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HHV9057689.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(7318..8085)
                     /locus_tag="ACUQPX_003059"
     CDS             complement(7318..8085)
                     /locus_tag="ACUQPX_003059"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HHV9057690.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(8291..8869)
                     /gene="lpcA"
                     /locus_tag="ACUQPX_003060"
     CDS             complement(8291..8869)
                     /gene="lpcA"
                     /locus_tag="ACUQPX_003060"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HHV9057691.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            9109..11553
                     /gene="fadE"
                     /locus_tag="ACUQPX_003061"
     CDS             9109..11553
                     /gene="fadE"
                     /locus_tag="ACUQPX_003061"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HHV9057692.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(11596..12042)
                     /gene="ivy"
                     /locus_tag="ACUQPX_003062"
     CDS             complement(11596..12042)
                     /gene="ivy"
                     /locus_tag="ACUQPX_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HHV9057693.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            12223..12993
                     /gene="yafV"
                     /locus_tag="ACUQPX_003063"
     CDS             12223..12993
                     /gene="yafV"
                     /locus_tag="ACUQPX_003063"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HHV9057694.1"
                     /translation="MPGLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            13103..13335
                     /locus_tag="ACUQPX_003064"
                     /pseudo
     CDS             13103..13335
                     /locus_tag="ACUQPX_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
BASE COUNT         3192 a         3442 c         3531 g         3223 t
ORIGIN      
        1 agctggattt aatgttgcgg tttataacgc atataaaaat taataaacca cgctccccgg
       61 cattcaacgc gctgctgctt aactgtctga aaaccataac gttcaaaaaa gggttttgcg
      121 gttatgcttg cgtccaccgt aagttcggat tcagacttaa tcaaaggttt taacaaagcg
      181 ctggcaaccc cacggcgggt gtattcaggg tcaacaaata acatatcgat atagtgttca
      241 acgcaggtaa taaagccgac cagttttgca ttaatgactg caacccgcac ttgtgatttc
      301 gcgagtttct ccttccagcg agattcgtca atctgcgccc aggcggcaat ttgttgtggt
      361 gaataatgct gactgacggt catcgtaacc gctctaatga aaatagcgca tagttgctga
      421 aaatcgccag gctgataatc tcttatttgt atgttattca taatataaat tcaaaaacgc
      481 atacgaaacg cttctgccat ttttccaatt ttatgcattt ggttgttatc tcgagccagt
      541 ttatgaggtt caggcttcag aatggcaatg agcaaccatg cttgctcatc aaacgcaccc
      601 tgacaatata ccaaatgcgc ttcgtcattc gttctgctga attggcgcaa ctgtggcgga
      661 aatggattat tcacatttgc cagatgaata tgagcaactc gctcaaattt gattaatggc
      721 caggtaaacg agtcgtcgta gagtgcatcg cgaccaaata tatctggcaa aacaccgtca
      781 cgcttatagg aaataaaatc cgccgttaac gcatcaagtt cctctgctgt aagttgcagg
      841 cgaataagtt ttgttttgaa tacccgcatc cttattcctt aaagtcattg aaatcatcat
      901 ccgtcatatc attaagatat tgtttagcgc ggcgtgtaat ttcctctaat cttgcagcac
      961 ttggacgaaa gcgcgccttt atgggctttt tctcctcttg ttcagcaagc atgtccatta
     1021 acgcttcgaa tgcgctggca cttaagagat atcctgcggg gcgattatta gaaagaaccg
     1081 caactggttg atcaataaag tatttagctg ggtttttacg taactcagtg atattgaccg
     1141 atttttcagc gagaattcga tgcatacagt gataccctca taataatgca caccagaata
     1201 tacataatag tatacatcat aatcccagca ccagttgtct ttccatttgc gggtcaagca
     1261 acgtcacatg cagccccacc agacgcacac cgcgcccgcc gcggcgttca tcccaggttt
     1321 tacgcgcggt ggcgattaag tcagctttat tcagccgcgg ccagacgtgc tcctgggtgg
     1381 tctgctgaaa atcgtcgaac tttaatttca ccccctggcg agcaatcagt aaatcaggtt
     1441 ttacctttgc cagacggcgt tcaagttccg gatacagccg ctcgataatc gcttcacatt
     1501 cagaccagtg atgaatatct tccgccatcg tgcgttccac gccgacggat tttcgtaacc
     1561 gttcgctgtt aacgtcgcgc tcgtcaatcc cctgactacg ctcccacaaa atgcggccaa
     1621 atttgccaaa gcgtttaagc agaatcacca gatcacactt ttgtacatca ccgcaggtcc
     1681 gcagccccat cgcttccagt tttgccgccg agactttgcc gacgccgggg attttttcca
     1741 gtggtaaggt ttgtaaaaat gccggaactt ctgccggcgt aatcacaaac tgaccgttgg
     1801 gtttattcat gtcggaggcg attttggcga gaaactttac tggtgccacg cccgcagatg
     1861 ccgtcagttg cagctcgttg aagatcgtct ggcggatttc ctgggcgatg agggtcgcag
     1921 aaccgtggca atggacgcta tcggtgacat cgagataagc ctcatccagt gacaacggtt
     1981 caatgcgcga ggtgtagcgc gagaagattt cacggatatg atttgaggct tctttgtagg
     2041 cgtcaaagcg ccccggaagc aaggtgagat gtgtgcataa tttgagcgcc atcccagtag
     2101 gcatagcgct acgtacgcca aatttacgcg cgggataatt ggcggtgctg atcacccccc
     2161 gacgttcgcg gctgccgcca atagcaatag ggatatcgcg cagggcggga ttgtcgcgca
     2221 tctccaccgc tgcgaaaaag cagtccatat ccacatgaat gattttacgc attgctcacc
     2281 tctcaacact ggtaaagtat acagtgattt caggttttga gaaatgcgta aagattcagc
     2341 atttagcgca ttcgctgcga aagcacctgc tgcttatcca gcttttgcac cagcggctgc
     2401 accactttat ccccatgctg accaaagtat tgatacagcg tatcggacgc acttttggtc
     2461 agcaccataa tctcaatacg ccgattgccc gcgctttgcg gattcttcgc gtccagcagc
     2521 atctggtccg ccattgcgct tacctgcatc actttatctt ccggcattcc ggcctcttcc
     2581 agcacccgac gagctgaaag cgcgcgatcg cccgaaaggt tccagttgtt gtagatattg
     2641 tttttgtagg ccatcgcatc ggtatgcccg gtaataataa ttttgttatc gagcgagtcg
     2701 aacactggcg cgagctccac cagcaaagtt ttaaagaacg gcataatctg cgcgctgccg
     2761 cgttcgaaca tattgcgatt ctggtcgtct ttaatcagca cgcgtaatct ctggggaaca
     2821 atctccatct ccagattcgc ttccatatgc gcatcacggg caatggtgtt gatactggtt
     2881 gccaactcgc caagctcggt ggcggatttc ttcttcagca gcgccgtttt ctcattcaca
     2941 tcacgcgctt ttttctccgt ctcttccggc accgccacgg tggcaggttt tggcggatga
     3001 gacgggctga ttttatttaa cggcgacagc ccaccaccgt taaaaatcga ctgcccgtgc
     3061 agcgcggcga taatgctttc acgctcggat ttactgacgc tgttgacaat caactttatc
     3121 gtcgtcacca aagaggccga gcgtatttcc gaggtttctg cccgctttac cctggacgcg
     3181 atgcccggca aacagatggc gattgacgcc gatcttaacg ccggattgat caaccaggcg
     3241 caggcgcaaa cccggcgtaa agatgttgcc agcgaggccg atttctacgg cgcgatggac
     3301 ggggcatcga agtttgtgcg cggagacgcc atcgccggga tgatgattct ggcgatcaac
     3361 ctgagcggcg gcgtctgtat cggcatcttc aaatacaacc tgagcgccga cgccgccttc
     3421 cagcaatacg tgctgatgac catcggcgac ggcctggtgg cgcagatccc ttccctgctg
     3481 ctctccaccg cggcggcgat tatcgtcacc cgcgtcagcg acaacggcga tatcgcccat
     3541 gacgtgcgcc accagttgct ggcaagcccg tcggtgctct acaccgccac cgggatcatg
     3601 ttcgtgctgg cggtggtgcc ggggatgccg catctgccgt ttttgctgtt cagcgccctg
     3661 cttggcttta ccggctggcg gatgagcaaa cgcccgcagg cggcggaggc ggaagagaaa
     3721 agcctcgaaa cgctgacccg caccatcact gaaaccagcg aacaacaggt cagttgggaa
     3781 actattccgc tgatcgagcc catcagctta agcctcggct acaagctggt ggcgctggtg
     3841 gacaaagcgc agggcaaccc gctcacccag cggattcgcg gcgttcggca ggtgatttcc
     3901 gacggtaacg gcgtgctgct gccggagatc cgcattcggg aaaacttccg cctcaagccc
     3961 agccagtacg ctattttcat caacggcatt aaggctgatg aagcggatat tccggcggat
     4021 aaactgatgg cgctgccctc cagcgaaacc tacggcgaga ttgacggcgt gctggggaac
     4081 gacccggcgt acgggatgcc ggtcacctgg atccagcctg cgcagaaagc gaaggctctg
     4141 aatatggggt atcaggtgat cgacagcgcc agtgtgattg ccacccatgt gaacaagatt
     4201 gtgcgcagct atattcctga tttgtttaac tatgatgaca tcacgcagtt gcataaccgt
     4261 ttgtcgtcga tggcaccgcg cctggcggag gatttaagcg cggcgctcaa ttacagccaa
     4321 ttgctgaaag tgtaccgggc gctgctgacc gaaggcgttt ccctgcgcga tatcgtcacc
     4381 atcgccaccg tgctggtcgc cagtagcacg gtgactaaag atcatattct gctggcggcc
     4441 gatgtgcgcc tggcgctgcg gcgcagcatt actcatccgt tcgttcgcaa gcaggagctg
     4501 acggtgtata cgctgaataa tgagctggaa aatctgctga ccaacgtagt gaatcaggcg
     4561 caacaggccg gcaaagtgat gctcgacagc gtgccggttg acccgaatat gctcaaccag
     4621 ttccagagca cgatgccaca ggtgaaagag cagatgaaag cggcggggaa agatccggtg
     4681 ctgctggtgc cgccgcagct acgccctttg ctggcgcgtt atgcaaggtt gtttgcgccg
     4741 gggctgcatg tgctgtcgta taacgaagtg ccggatgagc tggagttgaa gattatgggg
     4801 gcgttgatgt aagaatccgg catcggtgca caattgccgg atgcggcgta aacgccttat
     4861 ccggcctacc gttcaggcac aagtttgtag gcatgataag acgcgccagc gtcgcatcag
     4921 gcatctgcgc ataactgccg gatgcggcgt gaacgcctta tccggcctac cgttaaagca
     4981 cgatttgtag gccggataag cgtaagcgca tccggcacca gcgcacagtt ataaaatgat
     5041 acgctccccg gcacttaaat ccgttatgtc tcccgcccaa tcgatgggat ataaccctcg
     5101 cgcgacatcg cgatggaacg ttgagaatgg ccaatcactc acctgcttta cccagccatg
     5161 tttctctgga tttatataaa tataatcaac atgatggcga taatcttttg tattgcgaat
     5221 ggcatgctcc caaaaacgcg gttgccagat atttttcaat ccgcaagcat gggtgaactg
     5281 ctttttaatc tcccgccagc gagaggaaaa atcatcatcg ctttcgggta atgtccatat
     5341 gcaatgcata tgctccggca aaacgaccca ggcgttaatt tcaaaaggtc tgtctcgctt
     5401 aacgttaata atggcgttac ggagcgtctg gaactgggtg gtcagaagat ggcttcgacg
     5461 attgcgtaaa tttaccgtga aaaaccatgt tccccccttg atgtaataac gacgatattc
     5521 cgacatagcg actccttttt ctcaacggta agaggaaaag gaaaggccga caatgacgct
     5581 gtgttatatc gggaaagcgg ctcgcaaaaa cgcacagatt tgtaggtatg ataagacgcg
     5641 tcagcgtcgc atcaggcatc cgtgcacaac cgccggatgc ggcgtaaacg ccttatccgg
     5701 cctacaaaat catctcttcc gtcaaaatcc tgcgcgcgcc taaaaaatgg tcctgccaga
     5761 aaggttcggc taaccggctt acccgaatgg tttccccggt gcgcagtgac tcaataaact
     5821 gaccgtcgcc cagatacacc cccatatggt cggcaatctc gcggctgtgg atatggaaaa
     5881 atagcaaatc tccccggcgc aggtcgttat tcgccacaat cgttgcccga cggtagtggt
     5941 acatctcatt ggccgtgcgc gggagtttcg cctcaaggat cttgttgtag gcataaaaaa
     6001 ctaacccact acaatcgaag ccttgatcgg gccgcgtgcc gccccagacg taaggcttgc
     6061 caagctgctg ctccaggcgg tgaatcgcca cttcggtgat gttatgcagg tgatcgctgc
     6121 ttaaaaagtg gttgttttcc gccagcgcct gccagcgtct gtcactcgcc ttgagcggcg
     6181 caggaaacca ttctggatgc tgttttatct gctcccggtt gtggtggcgc tggcgagtaa
     6241 agggagcaaa acgcataacg aaaacacgcc tggatgcaaa aatctgccga gcaagaacga
     6301 agacatgaaa gacataaacc ggctgaatga aggtgagaat tttaaaagac cggaggagta
     6361 aacgggatta ttaattgctt atttgcatat tactattgag atttcataat cttaggaaat
     6421 aaatgataca tgcgcttttc ggactattga aaactgcccc tgtgctaaat ttgtaaaccc
     6481 agcgccgaca ggcttgcgta aaactctttt ggtgataatg tgagccttcc aaatccagac
     6541 tattccgatt tgggatccat tttgccgtcc tagcgtgtgt aaccgtttta tcaaggaata
     6601 agcagtatgc gtaaaatcgc attaattctt gcgatgttgt tgatcccgtg cgtttcgttt
     6661 gccggtttgc tgggcagcag tagctcgacc acgccagtga gcaaagagta taagcagcag
     6721 ttgatgggat cccctgtcta catccagatc ttcaaggaag aacgtacgct cgatctttac
     6781 gtcaaaatgg gcgagcaata tcaactgctc gacagttata aaatctgtaa atattccggc
     6841 ggcttagggc caaaacagcg tcagggcgat ttcaaaagcc cggaagggtt ttatagcgtc
     6901 cagcgtaatc agttaaaacc agacagccgt tactacaaag cgattaatat tggattcccc
     6961 aatgcttatg accgtgcgca tggttatgaa gggaaatacc tgatgatcca cggcgattgt
     7021 gtatccatcg gctgctacgc aatgaccaat cagggtattg atgagatctt ccagttcgtt
     7081 actggtgcgc tggtgtttgg tcagccgagc gtgcaggtga gtatttaccc attccgcatg
     7141 accgacgcca atatgaagcg ccataaatat tccaacttta aggacttctg ggagcagctg
     7201 aagccgggct acgactactt tgagcaaacc cgtaagccac cgaccgtttc tgtggtcaat
     7261 ggccgttacg tggtcagcaa gccgttaagc cacgaagtgg tgcagccaca actggcatca
     7321 aactacacgc tccccgaggc aaaataagcg ccattcgcct ggcataattt tttgccaggt
     7381 ttcattgccc gtcagcggct gtgtcgcaat caccgtgacc acatcattcg gtgtggtctg
     7441 cgagctgaag tcgatttcca catcctgatc cagcaacgtt gccacgccaa acggcgcgcg
     7501 gcgggtgatc cagtgtaaat tagtcgagca atacgccatt acatagcgcc cgtccgaaag
     7561 cagcatgttg aaaacgccct tcgcccgcag ttcatccgcc agtgaggcga tatatttaaa
     7621 cactgccgcc atgttgcccg gcgtgcgcgg gtaacgctgc gttaatttat gcagcagcca
     7681 gcaaaaggct ttttcgctgt cggtttcacc gaccgggcgg aagttgccgg tttccagtga
     7741 tttgtagccc gtcagttgtc cgttatgggc ataagtccag ttacgccccc ataactcgcg
     7801 ggtaaatggg tgagtatttt ccagcgccac ctcgccccga ttagcctggc gaatatgagc
     7861 caccaccgaa caggatttta tcgggtagtc ctggacaagt ttggcgatgg gggaattaaa
     7921 gctgggttgt ggatctttaa atgtgcgaca gcctttacct tcgtaaaagg taatgcccca
     7981 gccatcttta tgtggcccgg ttccaccacc acgctgtaca agtccggtga aactaaagca
     8041 gatatcggta gggacgttgg cgctcatccc gagcaattcg cacatgggta acctccacaa
     8101 cagcgggagc gcatggaccc cggcgaagat tgatttaaga attttcaggt cggatgcggc
     8161 gtaaacgaat tattcggcct acggttcggg tacagatttg taggcatgat aagacgcgtc
     8221 agcatcgcat caggcatcag cgcacaagtg ccggatgcgg cgtaaacgcc ttatccggcc
     8281 tacgccagac ttacttaacc atctcttttt caattaactg gatcaggata tggatcactt
     8341 taatgtgaat ctcctgaatg cggtcggcat aaccaaagtg cggaacgcga atttcgatat
     8401 ccgccgtgcc agccatttta ccgccgtctt taccggtcag ggtgatcact ttcatcccct
     8461 tctcacgcgc cgctgcgatc gctttgatca cgtttgcaga gttaccggag gtggagatcc
     8521 ccagcagtac atcgccttcg cgacctaccg cttcaacgta gcgggagaaa atatcattga
     8581 aaccgaaatc attaccgacg caggaaatat gactaacgtc agaaatagca atcgccgggt
     8641 agcccggacg gttttcacgg tagcgaccgg tcaactcttc ggcaaagtgc atagcgtcgc
     8701 aatgggaacc gccgttgccg caggaaagca ctttgccacc ggctttaaag ctgtctgcta
     8761 acaggaccgc cgcgcgctga atggcgtgaa tattggcgtc atcttttaaa aagttagcca
     8821 gcgtttccgc cgcttcgttc agttcgttac gaataagatc ctggtacatg aggatatcct
     8881 tcagcataaa tgtaatagac aaaatgcagt gtaccggata ccgccaaaag cgagaagtac
     8941 gggcaggtgc tatgaccagg actttttgac ctgaagtgcg gataaaaaca gcaacaatgt
     9001 gagctttgtt gtaattatat tgtaaacaca ttgctaaatg tttttacatc cactacaacc
     9061 atatcatcac aagtggtcag acctcctaca agtaaggggc ttttcgttat gatgattttg
     9121 agtattctcg ctacggttgt cctgctcggc gcgttgttct atcaccgcgt gagcttattt
     9181 atcagcagtc tgattttgct cgcctggaca gccgccctcg gcgttgctgg tctgtggtcg
     9241 gcgtgggtac tggtgcctct gaccattatc ctcgtgccat ttaactttgc gcctatgcgt
     9301 aagtcgatga tttccgcgcc ggtatttcgc ggtttccgta aggtgatgcc gccgatgtcg
     9361 cgcactgaga aagaagcgat tgatgcgggc accacctggt gggagggcga cttgttccag
     9421 ggcaagccgg actggaaaaa gctgcataac tatccgcagc cgcgcctgac cgctgaagag
     9481 caagcgtttc tcgacggccc ggtagaagaa gcctgccgga tggcgaatga tttccagatt
     9541 actcatgagc tggcggatct gccgccggag ttgtgggcgt accttaaaga gcatcgcttc
     9601 ttcgcgatga ttatcaaaaa agagtacggc ggactggagt tctcagctta tgcccagtct
     9661 cgtgtgctgc aaaaactctc tggcgtgagc gggatcctgg cgattaccgt cggcgtgcca
     9721 aactcattag gcccgggcga actgttgcaa cattacggca ctgacgagca gaaagatcac
     9781 tatctgccgc gtctggcgcg tggtcaggag atcccctgct ttgcactgac cagcccggaa
     9841 gcgggttccg atgcgggcgc gattccggac accgggattg tctgcatggg cgaatggcag
     9901 ggccagcagg tgctggggat gcgtctgacc tggaacaaac gctacattac gctggcaccg
     9961 attgcgaccg tgcttgggct ggcgtttaaa ctctccgacc cggaaaaatt actcggcggt
    10021 gcagaagatt taggcattac ctgtgcgctg atcccaacca ccacgccggg cgtggaaatt
    10081 ggtcgtcgcc acttcccgct gaacgtaccg ttccagaacg gaccgacgcg cggtaaagat
    10141 gtcttcgtgc cgatcgatta catcatcggc gggccgaaaa tggccgggca aggctggcgg
    10201 atgctggtgg agtgcctctc ggtaggccgc ggcatcaccc tgccttccaa ctcaaccggc
    10261 ggcgtgaaat cggtagcgct ggcaacgggc gcgtatgctc acattcgccg tcagttcaaa
    10321 atctctatcg gtaagatgga agggattgaa gagccgctgg cgcgtattgc cgggaacgct
    10381 tatgtaatgg atgctgcggc atcgctgatt acctacggca ttatgctcgg cgaaaaacct
    10441 gccgtgctgt cggctatcgt taagtatcac tgtacccacc gcgggcagca gtcgattatt
    10501 gatgcgatgg atattaccgg cggtaaaggc attatgctcg ggcaaagcaa cttcctggcg
    10561 cgtgcttacc agggcgcacc gattgccatc accgttgaag gggctaacat tctgacccgc
    10621 agcatgatga tcttcggaca aggagcgatt cgttgccatc cgtacgtgct ggaagagatg
    10681 gaagcggcga agaacaatga cgtcaacgcg ttcgataaac tgttgttcaa acatatcggt
    10741 cacgtcggta gcaacaaagt tcgcagcttc tggctgggcc tgacgcgcgg tttaaccagc
    10801 agcacgccaa ccggcgatgc cactaaacgc tactatcagc acctgaaccg cctgagcgcc
    10861 aacctcgccc tgctttctga tgtctcgatg gcagtgctgg gcggcagcct gaaacgtcgt
    10921 gagcgtatct cggcacgtct gggggatatt ttaagccagc tttacctcgc ctctgccgtt
    10981 ctgaagcgtt atgacgacga aggccgtaat gaagccgatc tgccgctggt gcactggggc
    11041 gtacaggatg cgctgtatca ggcagaacag gcgatggacg atttactgca aaacttcccg
    11101 aaccgcgtgg ttgccgggct gctgaatgtg gtgatcttcc cgaccggacg tcattatctg
    11161 gcaccgtctg acaagctgga tcataaagtg gcgaagattt tacaagtgcc gaacgccact
    11221 cgttcacgta ttggtcgcgg tcagtacctg acgccaagcg agcataaccc ggttggttta
    11281 ctggaagagg cgttggtgga tgtgattgcc gccgacccga ttcatcagcg gatctgtaaa
    11341 gagctgggta aaaatctgcc atttacccgt ctggatgaac tggcgcacaa cgcgctgacg
    11401 aaggggctga ttgataaaga tgaagccgct attctggtga aagcggaaga aagccgtctg
    11461 cgcagtatta acgttgatga ctttgatccg gaagagctgg cgacgaagcc ggtaaagttg
    11521 ccggagaaag tgcggaaagt tgaagccgcg taattgaata acggagccga aaggctccgt
    11581 ttctttatcc gctaattatt taaaattaaa gccatccgga tggttttcca ggctgccggt
    11641 caacgccgcg aacagcaccg ttttaccatc aatcgaaagc gcatcgttca cattcagcca
    11701 ggtgagtttc tcttgcgacg ttttctcatc aatagccgag aacagccccg tcatctgatt
    11761 agatttctcg gaccacatca cagcgatacg ttgcgagcca cagtcatgcg gtttgcacgc
    11821 gctcatcacc tgatacgtct catctcccaa cgttacggtt tgtgcgggag tataagtacc
    11881 gcctttcatc acccatgcag gtagcttatg cccttgcacc atctgattaa acgcagcttt
    11941 ggtggtttcg ccctttgcaa ggctgctaat ggttaaatca tcctgcgcca ttgcactggt
    12001 ggcgatgacc agtgcggcga ctgtcgttat tgccttaaac atcattcctc ccgagcttat
    12061 cctgcccatg ttattaacct cctaaagcct ggcgataaca cgataatttg caaatcagat
    12121 atttccaata tcagcatttg ttatcaattt attctgcgct aaggcaactg cctgataagg
    12181 tgttaaagtt ataaaaccct tcgcataaag gagttgtaca ccgtgcctgg tttgaagatt
    12241 acgcttttgc agcaaccgtt ggtgtgggtg gatggtcctg ccaacctgcg ccattttgat
    12301 cgtcaactgg aaggtattac cgggcgcgat gtgatcgttc taccggagat gtttaccagc
    12361 ggctttgcca tggaagcggc agcttcgtcg cttgcacaag atgacgtagt gaactggatg
    12421 acagctaagg cgcagcagtg caatgcgctg attgcaggca gtgttgcatt acaaacggag
    12481 tctggttcgg ttaaccgctt tttgctggtt gagccgggcg gcacggtaca tttttatgat
    12541 aagcgtcatc tgttccgcat ggcagatgag catctgcatt ataaagcggg caatgcgcga
    12601 gtgattgtgg aatggcgcgg ctggcgtatt ttgccgctgg tgtgctacga cttacgtttt
    12661 ccagtgtggt cgcgcaatct caacgattat gacctcgccc tgtacgtcgc caactggcca
    12721 gctccgcgct ctctgcactg gcaggcattg ctgacggccc gcgcgattga gaatcaggcg
    12781 tatgtggcgg gatgcaatcg cgtcggcagc gatggcaacg gctgccatta tcgcggtgac
    12841 agccgggtga ttaatccgca aggcgagatt atcgctaccg ccgacgcgca taaggcaacg
    12901 cgtattgatg cggagctgtc gatggtggca ttgcgggaat atagagaaaa gtttccggca
    12961 tggcgggatg cggatgagtt taggttgtgg tgagtggggg ttgtgtttag ggccgggggc
    13021 tgggctagat tacgaaagcc cctaacttaa acacaatatt acgccagaag tcaccatttc
    13081 caaaacacag cgtcgctgta ccgtgggtta tctgcccaac cgcagcgaca cgccggtgat
    13141 caaaatcagc gccaaatggc tgagaaaagc gggatttgag atcggcacgg gcgttaccgt
    13201 gaagatctcg gagggatgtt tgatcctgct tgggataaca acgaagtaca ggaactgcgc
    13261 agggaacttt atcaggttaa acagtcagtt aaggggatgc gagacgggat gtttagtgtg
    13321 ctgaacgaaa gttaaataat aaaaagccgg aaagatctgg aagaaatttc cggttattat
    13381 cgttgcta
//
LOCUS       DBKEKI010000107        13236 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.107, whole genome shotgun sequence.
ACCESSION   DBKEKI010000107 DBKEKI010000000
VERSION     DBKEKI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.107"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..325)
                     /locus_tag="ACUQPX_003065"
     CDS             complement(<1..325)
                     /locus_tag="ACUQPX_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057695.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNVRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIATI
                     FEEYLM"
     gene            complement(642..1466)
                     /gene="iclR"
                     /locus_tag="ACUQPX_003066"
     CDS             complement(642..1466)
                     /gene="iclR"
                     /locus_tag="ACUQPX_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HHV9057696.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1666..5349
                     /gene="metH"
                     /locus_tag="ACUQPX_003067"
     CDS             1666..5349
                     /gene="metH"
                     /locus_tag="ACUQPX_003067"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HHV9057697.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQVESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYARDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTQPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETCTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5569..7200
                     /locus_tag="ACUQPX_003068"
     CDS             5569..7200
                     /locus_tag="ACUQPX_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HHV9057698.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7291..7968)
                     /gene="pepE"
                     /locus_tag="ACUQPX_003069"
     CDS             complement(7291..7968)
                     /gene="pepE"
                     /locus_tag="ACUQPX_003069"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HHV9057699.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFGVTQTWDD
                     YTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGLLAPI
                     TDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTNALPE
                     GHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGEEAVP
                     LEAGHRF"
     gene            complement(8350..9579)
                     /gene="sorE"
                     /locus_tag="ACUQPX_003070"
     CDS             complement(8350..9579)
                     /gene="sorE"
                     /locus_tag="ACUQPX_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HHV9057700.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            complement(9631..10419)
                     /locus_tag="ACUQPX_003071"
     CDS             complement(9631..10419)
                     /locus_tag="ACUQPX_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HHV9057701.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            complement(10430..11227)
                     /locus_tag="ACUQPX_003072"
     CDS             complement(10430..11227)
                     /locus_tag="ACUQPX_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HHV9057702.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            complement(11293..11787)
                     /locus_tag="ACUQPX_003073"
     CDS             complement(11293..11787)
                     /locus_tag="ACUQPX_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HHV9057703.1"
                     /translation="MNITLARIDDRLIHGQITTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(11787..12194)
                     /locus_tag="ACUQPX_003074"
     CDS             complement(11787..12194)
                     /locus_tag="ACUQPX_003074"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HHV9057704.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNP
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            complement(12204..13010)
                     /locus_tag="ACUQPX_003075"
     CDS             complement(12204..13010)
                     /locus_tag="ACUQPX_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHV9057705.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(13080..>13236)
                     /locus_tag="ACUQPX_003076"
     CDS             complement(13080..>13236)
                     /locus_tag="ACUQPX_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HHV9057706.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
BASE COUNT         3162 a         3419 c         3483 g         3172 t
ORIGIN      
        1 ccattaggta ttcttcaaat attgtagcaa tggtttgagt gagtgtcgtg ttttcgtttt
       61 tattaattat cgtaatttct ttcttatcag cttgtaagac ggacgtggaa aatttaatga
      121 caaaatcctt gttgctaaag atatgacgaa cattttgtct acagttctct gagaagcttt
      181 ttaatagagg cgtcgccagc tccttgccag aaaatttatc ctcgagttct ttataaaaca
      241 attcactcag ggtttggtgt tcatttgtct gggctgtatt attaatattt gcagagaaag
      301 aactacgagg aatacgagta atcatttaaa tatctcattg tttattgatg tcttgaattt
      361 taactatcag aacagtgaaa aaatttaata tgattacaac taaaaaaata tcataaatcg
      421 ctcaatctca taatgcagcc gtaaaagttt cggtggaatg agatcttgcg attttgttaa
      481 taattaagtg gtttgatgtt taaaaataat gctgagttaa taaaagtgtg tgaagtgtat
      541 gacattaagt tattttggcg tcaatgcgat taacagacac ccttattcta ttgccactca
      601 ggtatgatgg gcagaatatt gcctctgccc gccagaaaaa gtcagcgcat tccaccgtac
      661 gccagcgtca cttccttcgc cgctttaatc accatcgcgc caaactcggt cacgcgatca
      721 tcggtaatac gtgaaatcgg tccggaaata gaaattgcgg caaacggttc gcggtgctca
      781 tcgaaaatac acgctgcaag gcaacgtagc cccagcgcat gttcctcatc gtcaaatgaa
      841 taaccccgtt tgcgcgtttg ggcgagatct tcttttaaat gcacaggaga caccagagtt
      901 gcgtgggtat aggcatgtaa ccctttgcgg tgcagcagct tcgtcacctg ttcttcgctc
      961 agttgggcta aaaaggcttt acccgcaccg gaagcgtgca tcggcaattt accgccgata
     1021 ggcgcggaca ttcgcatcag gtgggtacac tgtacctggt cgataataat cgcttcgtga
     1081 tcgctttgat caagcaccgc catattgacc gtttcgccag actcttccat tagattgcgc
     1141 aggatagggt gaacaattgc taacaaatta cggctctgga gaaagctgct gccgaccata
     1201 aaggcatgtg cgccgattgc ccagtgtccc agttcgccga cctgacgcac gaaaccctgc
     1261 tgttgcatcg tggttagcag gcggtgggtc gtggaattgg gtaacccggc ttgttgcgcc
     1321 agctctgtga gtgccacgct gccattggat tcggcaatcc actccagtaa tttcaggcca
     1381 cgcgttaaag actgaacctg tccagtcgct ggtgcggtgg caacggcggg ttttctgccg
     1441 cgtttcgcgg gaatgggtgc gaccatgaca gcctcctttt tctgtatcgt ggaaattatt
     1501 ttcattttta ttgttagcta atgcaatagt tgctgaactg atccgatgag ttaatgttga
     1561 acaaatctca tgttgcgtgg tggtcgcttt taccacagat gcgtttatgc cagtatggtt
     1621 tgttgaattt ttattaaatc tgggttgagc gtgtcgggag caagtgtgag cagcaaagtg
     1681 gaacaactgc gtgcgcagtt aaatgaacgt attctggtgt tggacggcgg tatgggcacc
     1741 atgatccaga gctatcgact gaacgaagcc gattttcgtg gtgaacgctt tgccgactgg
     1801 ccatgcgacc tcaaaggcaa caacgacctg ctggtactca gtaaaccgga agtgatcgcc
     1861 gctatccaca acgcctactt tgaagcgggc gcggatatca tcgaaaccaa caccttcaac
     1921 tccacgacca ttgcgatggc ggattaccag gtggaatccc tgtcggcgga aatcaacttt
     1981 gcggcggcga aactggcgcg cgcctgtgct gacgagtgga ccgcgcgcac gccggagaaa
     2041 ccgcgctacg ttgccggtgt tctcggccca accaaccgca cggcgtctat ttctccggac
     2101 gtcaacgatc cggcattccg caacatcact tttgaccagc tggtggcggc ctatcgcgaa
     2161 tccaccaaag cgctggtgga aggtggtgcg gatctgatcc tgattgaaac cgttttcgac
     2221 acccttaacg ccaaagcggc ggtctttgcg gtgaaaacgg agtttgaagc gctgggcgtt
     2281 gagctgccga ttatgatctc cggcaccatc accgacgcct ccgggcgcac gctctccggg
     2341 cagaccaccg aagcatttta caactcattg cgccacgccg aagcgctgac ctttggcctg
     2401 aactgtgcgc tggggcccga tgaactgcgc cagtacgtgc aggaactatc acggattgcg
     2461 gaatgctacg tcaccgccca cccgaacgcc gggctgccta acgcctttgg tgaatacgat
     2521 ctcgacgccg acacgatggc aaaacagata cgtgaatggg cgcaagcggg ctttctcaat
     2581 atcgtcggtg gttgctgcgg gaccacgccg caacatattg cagcgatgag tcgtgcagta
     2641 gaaggattag cgccgcgcaa actgccggaa attcccgtag cctgccgttt gtctggcctg
     2701 gagccgctga acattggcga cgatagcctg tttgtgaacg tgggtgaacg caccaacgtc
     2761 accggctctg ctaagttcaa gcgcctgatc aaagaagaga aatacagcga ggcgctggat
     2821 gtggcgcgtc agcaggtgga aaacggcgcg cagattatcg atatcaacat ggatgaaggg
     2881 atgctcgatg ccgaagcggc gatggtgcgt tttctcaatc tgattgccgg tgaaccggat
     2941 atcgctcgcg tgccgattat gatcgactcc tcaaaatggg atgtcattga aaaaggtctg
     3001 aagtgtatcc agggcaaagg cattgttaac tctatctcga tgaaagaggg cgtcgacgcc
     3061 tttatccatc acgcgaaatt gttgcgtcgc tacggtgcgg cagtggtggt gatggccttt
     3121 gacgaacagg gacaggccga tactcgcgca cggaaaatcg agatttgccg tcgggcgtac
     3181 aaaatcctca ccgaagaggt tggcttcccg ccagaagata tcatcttcga cccgaacatc
     3241 ttcgcggtcg ccaccggtat tgaagagcac aacaactacg cgcgggactt tatcggcgcg
     3301 tgtgaagaca tcaaacgcga actgccgcac gcgctgattt ccggcggcgt atctaacgtt
     3361 tctttctcat tccgtggcaa cgatccggtg cgcgaagcca ttcacgcagt gttcctctac
     3421 tacgctattc gcaatggcat ggatatgggg atcgtcaacg ccgggcaact ggcgatttac
     3481 gacgacctac ccgctgaact gcgcgacgcg gtggaagatg tgattcttaa tcgtcgtgac
     3541 gatggcactg agcgtttact ggagcttgcc gagaaatatc gcggcagtaa agcggacgac
     3601 actgctaacg cccagcaagc ggagtggcgc tcgtgggacg tgaataaacg tctggaatac
     3661 tcgctggtca aaggcataac cgagtttatc gagcaggata ctgaagaagc ccgccagcag
     3721 gctacgcgcc cgattgaagt gattgaaggc ccgttgatgg acggcatgaa cgtggtcggc
     3781 gatctgtttg gcgaaggcaa aatgttcctg ccacaggtgg tcaaatcggc gcgcgtcatg
     3841 aaacaggcag tggcgtatct cgaaccgttt attgaagcca gcaaagagca gggtaaaacc
     3901 aacggcaaga tggtgatcgc caccgtgaaa ggcgatgtcc acgacatcgg taaaaatatc
     3961 gttggtgtgg tgctgcaatg taacaactac gaaattgtcg atctcggcgt tatggtgcct
     4021 gcggaaaaaa ttctccgtat cgccaaagaa gtgaatgctg atttgattgg cctttcgggg
     4081 cttatcacgc cgtcgctgga cgagatggtt aacgtggcga aagagatgga gcgtcagggc
     4141 ttcaccattc cgttactgat tggcggcgcg accacctcaa aagcgcacac ggcggtgaaa
     4201 atcgagcaga actacagcgg ccctacggtg tatgtgcaga atgcctcgcg caccgttggt
     4261 gtggtagcgg cgctgctttc cgatactcag cgtgatgatt ttgtcgctcg tacccgcaag
     4321 gagtacgaaa ccgtgcgtat tcagcacggg cgcaagaaac cacgcacacc accggtcacg
     4381 ctggaagcgg cgcgcgataa cgatttcgca tttgactggc aggcttacac gcagccggtg
     4441 gcgcaccgtc tcggcgtgca ggaagtggaa gccagcatcg aaacgctgcg taattacatc
     4501 gactggacac cgttctttat gacctggtcg ctggccggga agtatccgcg cattctggaa
     4561 gatgaagtgg tgggcgttga ggcgcagcgg ctgtttaaag acgccaacga catgctggat
     4621 aaattaagcg ccgagaaaac gctgaatccg cgtggcgtgg tgggcctgtt cccggcaaac
     4681 cgtgtgggcg atgacattga aatctaccgt gacgaaacgt gcacccatgt gatcaacgtc
     4741 agccaccatc tgcgtcaaca gaccgaaaaa acgggattcg ctaactactg tctcgctgac
     4801 ttcgttgcgc cgaagctttc tggtaaagca gattacatcg gcgcatttgc cgtgactggc
     4861 gggctggaag aggacgcact ggctgatgcc tttgaagcgc agcacgatga ttacaacaaa
     4921 atcatggtga aagcgcttgc cgaccgttta gccgaagcct ttgcggagta tctccatgag
     4981 cgtgtgcgta aagtctactg gggctatgcg ccgaacgaga acctcagcaa cgaagaactg
     5041 atccgcgaaa actaccaggg catccgtccg gcaccgggct atccggcctg cccggaacat
     5101 acggaaaaag ccaccatctg ggagctgctg gaagtggaaa aacatactgg catgaaactc
     5161 acagaatctt tcgctatgtg gcctggtgca tcggtttcgg gttggtattt cagtcatccg
     5221 gacagcaagt actacgcggt ggcgcaaatt cagcgcgatc aggttgaaga ctatgcccgc
     5281 cgtaaaggta tgagcgtctc cgatgttgag cgctggctgg caccgaatct ggggtatgac
     5341 gcggactgat tcacaaatct gtcacttttc cttacaacaa acagggcgct caatgagtgc
     5401 cctgtctctt tattaatatg aaacacttat actggaaaca ggctggaata atcttagccg
     5461 gaaaaacgaa tgtcaaaagg cacagtaaag atgccagtca gcgtgtttcc ggtcctttgg
     5521 tcattcaatt gcttcaggaa catataacga taaggagaac ctgaactcgt gttaacgctg
     5581 cttcacctgc tttctgccgt cgccctgctg gtctggggga ctcatattgt tcgaaccggc
     5641 gtaatgcgcg tcttcggcgc gcgtttgcgt actgtcctta gccggagcgt cgaaaaaaag
     5701 ccgctcgcct tttgcgcggg gatcggcgtt accgcactgg tacagagcag taatgccacc
     5761 accatgctgg tgacctcgtt cgtcgctcag gatctggtag ccctcgcacc ggctctggtc
     5821 attgtgctgg gggcagatgt cgggacggcg ctaatggcgc gtattctcac cttcgactta
     5881 tcctggctgt caccgttact tatttttatc ggcgtgattt ttttcctcgg acgcaaacag
     5941 tcacgcgccg ggcaactggg ccgcgtcggt attggtcttg ggctgatttt gctggcgctg
     6001 gagttgattg tgcaggccgt aacgccgatc acccaggcaa acggcgttca ggtgatcttt
     6061 gcctcgctga ccggcgatat tctgctggat gcgctgattg gcgcgatgtt cgccattatc
     6121 agctactcca gccttgctgc tgtactgctg accgcgactc tgaccgctgc aggcattatc
     6181 tccttccccg tggcgctctg tctggtgatt ggagctaacc tcggttccgg cctgctggcg
     6241 atgctcaaca acagtgccgc caatgccgca gcccgccgtg tcgcgctggg tagtctgctg
     6301 tttaagctgg tgggtagcct gattatcctg ccgtttgtcc atttgctggc agagacaatg
     6361 gggaagctgt cattgccaaa agcggaactg gtgatctatt tccacgtctt ctacaacctt
     6421 gtacgttgcc tggtcatgct gccatttgtt gacccgatgg cacggttttg caaaacgatt
     6481 attcgcgatg aaccggaact ggatacccag ctacggccta aacatctgga tgtcagcgcg
     6541 ctggacacgc ccacgcttgc tctggcgaac gccgcgcgcg aaaccctgcg cattggcgac
     6601 gccatggaac agatgatgga agggctgaat aaagtgatgc acggcgagcc acggcaggag
     6661 aaagagctgc gtaagctggc agatgatatc aacgttctct acaccgccat taagctgtat
     6721 ctggcgcgga tgccaaaaga agagttggcg gaggaagagt cgcgccgctg ggcggagatc
     6781 atcgaaatgt cgctcaacct tgaacaggcc tccgatatcg tcgagcgcat gggcagcgaa
     6841 attgccgata aatcgctggc agcacggcgg gcattttcgc ttgatgggtt gaaggaactg
     6901 gatgcgctct atgagcaatt gctcagtaat ttaaagctgg caatgtcggt gttcttctct
     6961 ggcgatgtca ccagcgctcg tcgtttgcgt cgcagcaaac atcgttttcg cattcttaat
     7021 cgccgctatt cccacgccca cgtcgatcgc ctgcatcagc aaaacgtgca gagcattgaa
     7081 accagttcgc tacatttagg cttactggga gatatgcagc gcctgaactc gctgttttgt
     7141 tcggtggctt acagtgtgct ggaacagccg gatgaagatg aaggacggga cgagtattaa
     7201 tattgtgaat cctgtaggcc tgataagacg cgtcaagcgt cgcatcaggc actatgaaca
     7261 agatgccgga tcatgcatcc ggcaattcac ttaaaaacgg tgaccagctt ccagcggaac
     7321 cgcttcttca ccagccttaa acacataagt ggtgttcggg ccacccagcg tagcgtgacc
     7381 tttactcact gtgatccagt taccttccgg cagaccaata atcgtcagtt ctggcgcgac
     7441 gaccagcagt tcgcgaatac gctgctcacg ggtttcacct ttatggcctt ccggcagcgc
     7501 gttggtgaag tgcgggttga tttgcaacgg gaacagattt agcgcatcga aaccttgcgg
     7561 atcgacaatc ggcatatcgt tggtggtacg aatggttggg caagcaaggt tagcgcctgc
     7621 gctccagcca atatacagcg cgccacgttt aaccacgtca gtaattggag ccagcagccc
     7681 gcgctcgcgg cactgtttca gcaactggaa agtattaccg ccgccgacaa tcacaatttc
     7741 cgcattttca atcgcggcaa cgggatcgac aacgctatga ataccggtga cagaaacacc
     7801 cagcggagcg agaaccgcag ccgttttctc tgtgtaatca tcccaggtct gcgttacgcc
     7861 aaacaccgct gagcggcgac cctgcaactg ttcagcaatt agcggcagtg catgttccag
     7921 ccaggcttta cccggcagcg tcgagttact caataaaagc agttccatta cttctccggt
     7981 tagtaagaaa tcacggaagg catgctacca ctgcgaattt aaccacttac tgacatggct
     8041 cacgatgtta cgtattgcac cgggcgctga gactgatgac aaacacagca ttgcctgatg
     8101 cgctacgctt acctggccta ccagataact gcaatatatt gaattttcaa aattatgtag
     8161 accgaataag gcttcattcc gcatccggca taaacaatgt gtactttgcc accaatttga
     8221 gcgcccctta aggcgctgat attttttatc cttctcgtgc aaattgtgct gcttcagcaa
     8281 tcagccgcac atccggtcgc gaagttgctg gggcgaccag ccagcgacat gcagagttaa
     8341 gtagcgcgat taatcatggg aaattgcctc tgcatgggcg agcaggtatt gctccgcctc
     8401 accggaccag atcccctgat gacgcgccag aatcgccgcc agtgcttgat cctgaatctg
     8461 cgtgagtgac gtcagcggca ggtatttccc ggtatacacc agctttttgc cgccgccgac
     8521 ggcaggcaat tcaagcgtgg tttcgcccgc ggcattcagc ccaagaatat gtgttaccac
     8581 ttttgcggcc tgcacttttt tctcttcgat cagtttgacc gccgcgcgca tgtcgtcggt
     8641 attgccgcct gacgtgccca cgtagtgggt aaatgcataa tgcacatcgt agaaattaat
     8701 tggcgcgctg aaatgtttat cctgcggtcc ggcgaagaaa ttcaggcaac catctgtcgc
     8761 cagcaaggaa gaggcgagag tcaccagtcc ttcattaggc acaaagacga aaatatcatc
     8821 gaagccgtga ccgccactca gcgccattaa cgtatcaaat gctgcatcgg tggcattgag
     8881 ataatgaatc agtgtttgcg gttctgatgg atagtgcttg cgcgcataac tcaatttgtc
     8941 gttgtcggta tcggtaatga cgagcagcga cgggttaact ggtccatgca gcgcataatc
     9001 aatcgccaac agtcccattg gtccggtgcc gccgaggatc agcgtgcgcc cttgcgggcg
     9061 aatccccatc gtgtggttat aactaccttc ctgaagatga tagttggcgt tgaacgcgcc
     9121 aatcacgcag gaaagcggtt caaccagtga gccttcaaaa taggtttcgc cgtcgtatgc
     9181 cagcaggcaa tcttgttcca tgacctcgtt gggaataacc acatgcgtcg cttcgccgcc
     9241 tacccacggg aaggagtagc cggggcaatc cgggcggtcg gggagttgca ggttggcctg
     9301 aatcacataa cgctgacccg gctggaattt gtgctgccac tttttaccca cggccagaat
     9361 atcgccgcaa aattcgtggc cgatgatgat tgggttggtc gccacatcgt cgggtacttt
     9421 tttatggttt tcacccagat tggcctcttt ccaggaagag aggcacaggc tgtcagtgac
     9481 caccgttgcg aggatttcat cctcctgcat ttcaggaagg tcaaaggttt ccaggcgtaa
     9541 atcacgttta ccataaagac gcagagctgt tgttttcata aaccacctca tgttgagcga
     9601 tggcctcggc agtcgtgccg gggcgaagaa tcaggacagg aagcccagag cattgccgat
     9661 aatccctaag ccaaacaggg cgaaaatcag ccataccggg ttaatttttt tgttgagcag
     9721 acgaaccatt agtagcgtca gaccgagcgc cagcaaacca gggcaaagtt ggtcgagaat
     9781 gttctgcacg gtcatggtga cggtggagcc atcggcggca tgcgtttgtg aaaccaccaa
     9841 cggtacgttg attgacgtcc atttggtcac cagcacgccc atcacaaaca gtccgagaat
     9901 tgacgcgcct tcggtgagtt tttgcagcac attcccgccc atatcgctga cgatattcac
     9961 ccctttgcga aagccgagct gtaggccata ccacttcatc gccagacgca ccgcgttgaa
    10021 aataaagaag aacagcagcg ggccgagaat gttgcccgaa agtgccagag atgcgccgag
    10081 cgcggcggta atcgggcgca gcgttcccca gaccagtgga tcgccaactc ctgccaatgg
    10141 tcccatcaga ccgactttga tgccgttgat ggtaccgtca tcaatttccg cgccgatgac
    10201 cgggccacat acggctggcg tggtattgaa gaacaccagg tgtcgcctga gcgccgcaac
    10261 ctgatcctct tttaatgggt aaagtcgctt gatggcgggg atcatgtcgt agcaaaagcc
    10321 cagcccgtga atacgttcaa agttaaagga cgcctgttgc aggttggagc gcagaaacat
    10381 gctcaccaga tcgctgcgtg taatttttct ctgttccatg atggctccgt taatcgtcaa
    10441 gttggtcaag ggcggtggag gcggtggcct ggagttgagg ttcagcttta cgccactgtg
    10501 gatttagctg gatgtagacc agggccatga tcacgccgac gccaccgaac gccagcaggc
    10561 tgagatcgag gtagccacct gcgaggaaac cgaggaaaaa gaaaggcatc aaatatttca
    10621 cgcccatcat acgtagcacc atggcgtaac cgaccaccac gataaaaccg ccagcaatct
    10681 gtagtcctcg ggtcacaaat tcgggaatgg cgtttaacat attgctgacc atatcggcgc
    10741 tgacgaacag tgagacaatc agtgccggaa tggcgacgcg cagcgcctgc acgccaagcg
    10801 cggagacatg cagaatatcg agagtgcgaa accgcgcctc ttctgctgct ttatccgccg
    10861 cgtgctggaa cactacggtg atggtacggg caaacaccgt cagcacttgg cctgccgcag
    10921 ccacaggcaa cgcgatggcg attccggtgg cgatgctttg ctgaccaacg ataaccagaa
    10981 tggcggagat tatgctggcg agtgcagaat ccggagattg cgccgcgccg acgttcatcc
    11041 agccgagagc tatcaattcc agcgtaccgc cgagtattat tccggttttt aaatcaccga
    11101 gaattaaacc aatcaccgta caggcaatta acgggcgatg agtctgaaat tcatccagca
    11161 cgctgcccat tccggcaata caggaaaaaa gaaatatagc aatgatttgt agggtactta
    11221 tttccatgat ttgtcacctt ttaagtgttg tcatccctgc ctgttaaagc atatgtacac
    11281 aggcgctatt atttaatttg cgattaactg ttcgtttatt ttgtcgataa tattgattga
    11341 gggatctgac gctacgacgc gtaaatccag tatgacacca agattattta actcatgaaa
    11401 tgcgttaata tcatcatcat ctaatgaaac cgctttggtt aactgttttt taccgggccg
    11461 ccaggccatg ccgccaatat tcagggtgcc tatttttacc ccctggcgaa ccatcgctaa
    11521 ggcatcctgt gggcgagtga aaagataaaa aacggtttcg tcctgatatt gcgggttgtg
    11581 ataaaccgcg acggcttttt caatattgac cacgttaacc ttcattcccg gtggcgcagc
    11641 ctgacgcaat aatgtccggc gcacttcatc gttataaact tcgtcattgc agataataat
    11701 gcgctgggcg ttagccactt tcgaccagac ggtagtgatc tggccgtgga tcagacggtc
    11761 atcaatgcgg gcgagcgtaa tgttcatcag aaatcctcct ctgtcgttgc cagttgtttc
    11821 cagacaacgc aggtttgctt tgcgatttgc gtcaggtgtt cacacagttc atcaacattc
    11881 atgctgtccg ggttatcaac cagttcgagc gccagcggca gagaaaggcc gctgattacc
    11941 cgcaaacgag gattgcgcat cgcaagcatt gctgccgcat tccacgggct gccgcactgt
    12001 aaatccacgg caatcagcca ctcatcttga ttgtgaatgc ttactaactt ttccagcttt
    12061 accgcaatgt ctccggcgtt ttcaccgggc acaaattcaa cggcttcaac attggcgttg
    12121 ccgtaaacca tctgaaccga ttccagcatg gcgcaagcta atttgccgtg ggcacaaaaa
    12181 atggcgttga ccataaagcc tccttagccg cgcgttttgc cgcccgcaat gttagtggtt
    12241 actccggtga tgtagctggc gcgttcagac aacagataac aaacaaaatc agctacttct
    12301 gctaatcttc cggcgcgccc aataggaatg gcgtttttgg tatagccttc acgcagctgc
    12361 tcgacggtga tattgcgcgt ccacgccagc gcctcttcat attccggcgt gcgtagcccg
    12421 gttttttcca gaatcccggg cgcgataccg accacacgga taccgtgttt acccagctct
    12481 ttcgaccagg agcgcgtgaa gctattgagc gcggctttgg tagcggcgta acagctttgt
    12541 ccttctgagc cttccagccc actttctgag gaaacattca caatcacgcc atcatgttgt
    12601 ttgaccatct gtcgcgccac cgcctgcgac atcagaaaaa cacctttctg gttgatattg
    12661 accatttttt cgaatgcagc ttcgttgagt tcatactgcc cggcaggcgc tttctcatcg
    12721 accagcagac gcgggaaatt gaccccggcg ttattgacca gaccgtcgat gcgaccaaaa
    12781 cgctggataa tttccgctac cgtatgattt acctctttgg tgctagaaat atcggtcggc
    12841 caaaactgat agcctttatg accttcatat tgaccatcgc caccgtgaat atcgaccatc
    12901 tgtacattcg cgccttgtgc taataattcc tctacaatcg ccagaccaat accggatgcg
    12961 ccgccggtga caataatgat tttatcctgc aaatttaacc acgtttgcat aacagactcc
    13021 tgattttaga taatagggac ccgccgtaat ttttattacg gtaaacatcg ggaagatttt
    13081 tatttcagta atagttcagc tgtgctgcta ttcgttacca gacaattaat atattttcca
    13141 cgcagtgcac caataattcc gctgtatttt tcttcactca tggcaatgcc aatggaatat
    13201 cgcgcctgct ttaatttatt catttcgata gagagc
//
LOCUS       DBKEKI010000108        13144 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.108, whole genome shotgun sequence.
ACCESSION   DBKEKI010000108 DBKEKI010000000
VERSION     DBKEKI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.108"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..176)
                     /locus_tag="ACUQPX_003077"
     CDS             complement(<1..176)
                     /locus_tag="ACUQPX_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHV9057707.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLT"
     gene            267..431
                     /locus_tag="ACUQPX_003078"
                     /pseudo
     CDS             267..431
                     /locus_tag="ACUQPX_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
     gene            complement(539..940)
                     /gene="pliG"
                     /locus_tag="ACUQPX_003079"
     CDS             complement(539..940)
                     /gene="pliG"
                     /locus_tag="ACUQPX_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HHV9057708.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            1182..1475
                     /locus_tag="ACUQPX_003080"
     CDS             1182..1475
                     /locus_tag="ACUQPX_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HHV9057709.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            1547..2206
                     /locus_tag="ACUQPX_003081"
     CDS             1547..2206
                     /locus_tag="ACUQPX_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HHV9057710.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            2298..2744
                     /locus_tag="ACUQPX_003082"
     CDS             2298..2744
                     /locus_tag="ACUQPX_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HHV9057711.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2951..3851)
                     /gene="hlyE"
                     /locus_tag="ACUQPX_003083"
                     /pseudo
     CDS             complement(2951..3851)
                     /gene="hlyE"
                     /locus_tag="ACUQPX_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0090729 - toxin activity [Evidence IEA];
                     GO_process: GO:0044179 - hemolysis in another organism
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            4224..4643
                     /locus_tag="ACUQPX_003084"
     CDS             4224..4643
                     /locus_tag="ACUQPX_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HHV9057712.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            4643..5911
                     /gene="umuC"
                     /locus_tag="ACUQPX_003085"
     CDS             4643..5911
                     /gene="umuC"
                     /locus_tag="ACUQPX_003085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HHV9057713.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGRDIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMFSPRYTTRSSDLLRVK"
     gene            complement(5957..6487)
                     /gene="dsbB"
                     /locus_tag="ACUQPX_003086"
     CDS             complement(5957..6487)
                     /gene="dsbB"
                     /locus_tag="ACUQPX_003086"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HHV9057714.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(6633..8174)
                     /gene="nhaB"
                     /locus_tag="ACUQPX_003087"
     CDS             complement(6633..8174)
                     /gene="nhaB"
                     /locus_tag="ACUQPX_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HHV9057715.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            8395..9114
                     /gene="fadR"
                     /locus_tag="ACUQPX_003088"
     CDS             8395..9114
                     /gene="fadR"
                     /locus_tag="ACUQPX_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HHV9057716.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(9166..10698)
                     /locus_tag="ACUQPX_003089"
     CDS             complement(9166..10698)
                     /locus_tag="ACUQPX_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HHV9057717.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFLPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVQDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            11028..12326
                     /gene="dadA"
                     /locus_tag="ACUQPX_003090"
     CDS             11028..12326
                     /gene="dadA"
                     /locus_tag="ACUQPX_003090"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HHV9057718.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLVRMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFCDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            12336..>13144
                     /gene="alr"
                     /locus_tag="ACUQPX_003091"
     CDS             12336..>13144
                     /gene="alr"
                     /locus_tag="ACUQPX_003091"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HHV9057719.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDG
                     ISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIAN
                     TGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYLR"
BASE COUNT         3345 a         3094 c         3359 g         3346 t
ORIGIN      
        1 gtaagacttc cttcctggta ttgcttacgc cagagaaata actggctggc tgctacacca
       61 tgttgccggg caacgaggga gaccgtcatc cccggttcaa agctctgctg aacaattgcg
      121 atcttttcct gtgtggtacg ccgtctgcgt ttctccggcc ctaagacatc aatcatctgt
      181 tctccaatga ctagtctaaa aactagtatt aagactatca cttaaataag tgatactggt
      241 tgtctggaga ttcaggggcc agtctatcat tacaaggcta cgatcccagc gaatttacat
      301 tcgctaacgg tgtattttgc gatgttaaag aaaaattatg tcgtgatgat cgttattttg
      361 gtgtagatgg taagcggagt ggaaaaatca atcaaaccac cacaaagatg ttatttatgt
      421 gtcgtgagtg atgccttgtt tacattatgt tctctgctat tcgtcattaa cctcttgagc
      481 gacagagagg agtggggcgt taacgtctat gacggggata gtccccgtcg gttgacattt
      541 acttaatttg aatatcgacg ttatattttt tagttttgtt tttacgggcg tcgtttcgag
      601 tttgcagcac tcgcagctca tatttgccag aagcagggag tgagtattgt ccgtgactat
      661 cgagttctgg ggaatatctg gataaatcaa cagaatcatc gattcctggc ccgaagagat
      721 aagtatcagc gccttcatta gagatactca catgcacttt ctgacctttt ttggcataga
      781 aggtatatgt atcgtaatcg tatcccttta tttcgccgga gtactgagcg ctgctatggc
      841 ctttgctgaa ctcaacattg acattcttac ccgcagcaaa gctcgtggag gttagcagtg
      901 caagcagcaa tacagcctta ctgatgctct tgattttcat tggtagtctc ctgttcagaa
      961 tgagtacgat gaactgttat aatataacaa tcccttacgg gagaattcca gtggcttttc
     1021 tgcgttcggt aaaatcccgg cgcggcattt tttgccgggg ggattactta taaaggggca
     1081 tggactcaac aaaccgcaga aatctacagc tggctggctt gtggcgcggg tttcattgat
     1141 gttagcttat gccaaaacct ggcatcttaa agagtaaaag tatgttttgt gtgatttatc
     1201 gaagcagcaa gcgtgaccag acctatttat atgtcgaaaa aaaagacgat ttttcgcgtg
     1261 ttcctgagga actgatgaaa ggttttggtc agcctcagtt agcgatgatt ctgccgttgg
     1321 atgggcggaa gaaactggtc aatgctgata ttgaaaaggt taaactggca ttaaccgagc
     1381 aaggttacta tttgcagtta ccgccaccac ccgaagattt gctgaagcaa catctttccg
     1441 tcatggggca gaaaacagac gacactaaca aataaccgat atccggcggt ggcattatct
     1501 ttgtcggcgc gggttttcat atccacgata aggtgagggg aacgttatgt atcaacatca
     1561 caactggcaa ggtgcgctgc tggattatcc ggtgagtaaa gtagtctgtg ttggcagtaa
     1621 ctatgccaaa catattaaag agatgggcag cgcagtgccc gaagagccag tgctgtttat
     1681 taaaccagaa acggcactgt gcgatctgcg gcagccattg gcgatcccat ccgatttcgg
     1741 ttcagttcat catgaagtcg aactggcggt gttgattggc gcgacgctgc gtcaggcaac
     1801 ggaagagcat gtccgcaaag ccattgccgg ttacggcgtg gcgcttgatc tgacattgcg
     1861 tgatgttcag ggaaaaatga agaaagccgg gcagccgtgg gaaaaggcta aagcgtttga
     1921 taactcttgt ccgctttccg ggtttatccc cgcggcggaa tttaccggcg atccacaaaa
     1981 tacaacgctg ggcctgagcg taaacggcga acaacgccag caaggtacga ctgcggacat
     2041 gatccataaa atcgttccgt tgatcgctta tatgagcaag ttttttaccc tcaaggccgg
     2101 tgacgttgtg ttgacaggca cgcctgatgg cgtcggcccg ttgcaaagcg gtgatgagct
     2161 gacagtcact ttcgatgggc attctttgac aactcgcgtt ttgtaatact ttttgccgcc
     2221 agaaagcggc ggcaaaactt gcatcgctgt gccagactgg ttataaggtg cgttttaacg
     2281 taatggcgga acacctgatg agcgatgtac ctttctggca aagtaaaacc ctggacgaaa
     2341 tgagcgatgc ggaatgggag tcgttgtgtg atggttgcgg tcagtgttgc ctgcataaac
     2401 tgatggatga agacaccgac gaaatctact tcactaacgt cgcctgtcgc cagctcaata
     2461 ttaaaacctg tcaatgtcgg aactacgaac gtcgtttcga gtttgaaccc gactgcatta
     2521 aattaacccg tgaaaatctg ccaacattcg aatggctgcc aatgacctgc gcttatcggt
     2581 tgctggcgga aggtaaagat ttacctgcgt ggcatccgct acttactggt tcgaaagcgg
     2641 caatgcatgg tgaacgtatc tctgtgcgtc atatcgcagt gaaagaatca gaagtgattg
     2701 actggcagga tcatatctta aataaacctg actgggcgca gtgataaatt taatagatgc
     2761 tgatgggggc tttcttaata tacgtctgga tgctcgccat ttcgtagccc tgtttttgaa
     2821 agcgctctgt cataatgaga gttacccgat accttattat caacaggtaa ctctcaatct
     2881 ttaaaaaacg tctgtgtttt taaatgtgat aaaccttata ccttgagtac atggagagaa
     2941 taatcgctta tcagacttca ggtacctcaa agagtgtctt tttaccgtgt cttttctgat
     3001 actcattaca ggtgttaatc atttttttgg ccgcttcttt tagcaaagaa agcattaaat
     3061 catcataatc aacatagaat ctggttgttt cagtttccgt ttttatctcc ccgatggcgg
     3121 ctatttcggt ggttaatttc aatttggcgg catcgatatc tttattcgct tgtttaaccg
     3181 tgttagacag ggtggtaaag aaactctgca cagattttaa cttgttcttc aattctggaa
     3241 tcagtttccc ttcaactacg cccgcagcaa tagaatagga aatgattaaa ccaaatggac
     3301 cggcgacgac accggctgcg gcaccggcat acgcttcctt cctgatttta tctacctgtg
     3361 actggaaata gctgcttttt tctgaaaaat cattggttaa ctggctatct aacgccagca
     3421 gtttcccgga agcgttgttg aaactttgtg agcttaccag cagggatttt tgcgcttcat
     3481 tcagcttcgt gatgccgtca tccagtacct taatgagggg cggatgcttt cttctcattg
     3541 tactcatcaa atagcaaaat atacgctgcg agcaattgcg tcgcaacacc acaccattca
     3601 tacactgttt gggttgcttc aaaatactta ttctggctat ccataagtaa ggttttaata
     3661 tcgccgacta aaacggaggc tgcctgtgaa tactcctgtt taaagcgact taactctttt
     3721 atggtttcat caaaggtctg ccaggggatg acctgatcga gatatttatt ataaagatct
     3781 aatgctccat ctgcggtttc gattgcgttt ttaactactt ctaccgtttt atctgcaacg
     3841 atttcagtca taatcattcg cctctttaaa tatataaatt gtaatgaaac tcctgtttta
     3901 caactattaa taaattttac ttcatctaat tcatagttag ccgggcggga tgcgtcaatg
     3961 tctttatttc tattaatatg ataaatatca aacaatgttt aatgtcatta tggcgaatgc
     4021 ttctattcta tttttttgcc gggtgatatt tttcatttct gctggatgag cgtcgtcgcc
     4081 agaaggccac gtgagcacag gataagagaa cgaaaaatca gcagtctatg cagcgacaaa
     4141 tattgatagc ctgaatcagt attgatctgc tggcaagaac agactactgt atataaaaac
     4201 agtataactt caggcagatt attatgttgt ttatcaagcc tgcggatctc cgcgaaattg
     4261 tgacttttcc gctatttagc gatcttgttc agtgtggctt tccttcaccg gcagcagatt
     4321 acgttgaaca gcgcatcgat ctgaatcaac tgttgatcca gcatcccagc gcgacttact
     4381 tcgtcaaagc aagtggtgat tctatgattg atggtggaat tagtgacggt gatttactga
     4441 ttgtcgatag cgctattacc gccagccatg gtgatattgt catcgctgct gttgacggcg
     4501 agtttacggt gaaaaaattg caactacgcc cgacggtaca gcttattccc atgaacagtg
     4561 cgtactcgcc cattaccatc agtagcgaag atacgctgga tgtctttggt gtggtgatcc
     4621 acgtcgttaa ggcgatgcgc tgatgtttgc cctctgtgat gtaaacgcgt tttatgccag
     4681 ctgtgagacg gtgtttcgcc ctgatttatg gggtaaaccg gtggttgtgc tatcgaataa
     4741 tgacggttgc gttatcgccc gaaacgctga ggcaaaggcg ctgggcgtta aaatgggcga
     4801 tccctggttc aaacaaaaag atctgtttcg tcgctgtggt gtggtttgct ttagcagcaa
     4861 ttatgagctt tacgcagaca tgagcaatcg ggtgatgtcg acgctggaag aactatcgcc
     4921 ccgcgtcgag atttacagta ttgatgaagc attctgcgat ctgacaggtg tgcgtaattg
     4981 tcgcgatctg actgattttg gcagagatat tcgcgcaacg gtgctacaac gtacccatct
     5041 tactgttggt gtggggatcg cccagaccaa aacgttggct aagcttgcca atcatgcggc
     5101 aaaaaaatgg cagcggcaga cggggggggt ggtggattta tcaaatctgg aacgccagcg
     5161 taaattaatg tctgctctcc ccgtggatga cgtctggggg attggacggc ggatcagcaa
     5221 aaaactggac gcgatgggga tcaaaaccgt tctcgatttg gcggatacag atatccggtt
     5281 tatccgtaaa cattttaatg tcatgctcga aagaacggtg cgtgaactgc gcggcgaacc
     5341 ctgtttgcaa ctggaagagt ttgcaccgac gaagcaggaa attatctgtt cccgctcgtt
     5401 tggtgaacgc atcacggatt atacgtcgat gcggcaggcc atttgtagtt acgctgcccg
     5461 ggcggcggaa aaacttcgca gtgagcatca atattgtcgg tttatctcca catttattaa
     5521 gacgtcacca tttgcgctca atgaacctta ttacggcaat agcgcgtcgg taaaactgct
     5581 gacgcccact caggacagca gggatatcat taacgctgct acgcgatctc tggatgccat
     5641 ctggcaagcg ggccatcgct accaaaaagc gggcgtgatg ctgggggatt tcttcagtca
     5701 gggagtcgcg cagctcaatt tattcgatga caacgcaccg cgccccggga gtgagcaatt
     5761 gatggcggta atggatactc tgaatgctaa agagggcaga ggaacactct attttgccgg
     5821 gcaggggatc caacaacaat ggcagatgaa gcgagctatg ttttcaccac gttatacaac
     5881 gcgaagttct gatttactgc gggtcaaata aatatagcgg caggaaaaaa gcgctcccgc
     5941 aggagcgccg aatggattag cgaccgaaca gatcacgttt tttcgcttta aacggctggg
     6001 aaatcaccac cagcaccgcg acaatcaggt aagcgataaa aataccgagc agccactgcg
     6061 gcatttccat acctaaaaaa tcccactgac gctcggcgca atcgccagag gcgacaaaca
     6121 cttgcggcac ccatttatcc agcggcagcc attccgggaa acgaaccata aaatcacagg
     6181 tggcaaatgg cgaaggatag agctgaagca tggtgtgctc gtaagttaac tgcacaccgc
     6241 ggaacgcact atacaaccag ataaccatcg ctacataacg cagcggagtt ttcggggcga
     6301 tcgcgccaat cagcgcagca cccagaacgc cgaataacgc gcagcgttca taaatacaga
     6361 gcacgcaagg tttcagtaac atcacatgct ggaaccacag cgccgtcagt tccagtgcca
     6421 gagcagtaaa cgccatcaac agccacgcgc cccggccttg tgaacattgg ttcaaaaatc
     6481 gcaacataat catttccctg caatatgcat agagtgcgca gtttaaacca attcattcgc
     6541 tgcgccacca ggaggcagca aaaataggac gcaattggac aattatcggg cgaaaaggca
     6601 aaccgggcat ttatatgccc ggtaagttgt tatcaaagcg ttgctatcca gcccatttgc
     6661 ataaaccatt cggttacagg ggcaagcgta aactcgacgc agagcaagcc gacgagtgtc
     6721 agtacgaggg tgtaaggcag ggccatccac accatgcggc cataagagag gcgaatcaat
     6781 ggcgcgagtg cagaggtcag caggaacagg aacgcagcct gaccgttcgg cgtagcgaca
     6841 gagggcagat tggtaccagt attaatggcg accgccagca gctcgtattg cttcaacgtg
     6901 atagcgccac tttccattgc cgctttcgct tcgttgatat aaatcgtccc cacgaagacg
     6961 ttatccgaaa tggatgacag cagaccgttg aaaatataga acagcgacag ctgagcatgt
     7021 tcagatgcct gcaacacaaa ctgaataatc ggcgaaaaca gttgttggtc gataatcacc
     7081 gcgactaccg agaaaaacac cgtcaacagt gcggtgaatg gcagagattc ggtgaaggct
     7141 ttaccgatag catgttcatc ggtgacaccg gtcaatgatg ttgccagaat aatgactgac
     7201 aaaccaatca agccaacttc cgccagatgc aacgccagcg cagtcaccag ccagacgcca
     7261 ataatcgcct ggacaatcag acggatttta tcctgacggg tacgctggtg gcggctttga
     7321 tcgtcaaact gttgcaacac ttcgcggact ttctccggca gcgtttcacc gtagccaaac
     7381 caacgcagct tctctaccag caggcaggtt aacaggccac aaatcagaac cggaacggtc
     7441 accggcgaca tgcgcaggaa gaaatcgcca aaatgccagc cagccgcttt agcgatgatc
     7501 aggttctgtg gttcgcccac catggtcatt acgccgccta atgcggtgcc gacaccggca
     7561 tgcatcatca ggctacgcag aaagccacgg aactgttcca gaaccacttt gtaatgcttg
     7621 tcgatatgac tatcgtcttg caggtcggtg tcttcggtac gggaagaggc tacgcgatga
     7681 taaataccat aaaaaccgac tgcaacgctg atcaccaccg ccacgacggt taaggcatcg
     7741 aggaacgcgg agaggaacgc tgccgccacg caaaaagaga gcgacagcag cattttggag
     7801 cgaatgctta acagcaaacg ggtaaatatg aacagcaaca gctgtttcat aaaatagata
     7861 cccgccacca taaacatcaa taacagcaag acttcaagat ttgccgccac ctcttcacgg
     7921 acgtgttccg cgctggtcat gccgatgaat accgcttcga tagccaacag accaccgggg
     7981 agtagcgggt agcatttcag ggccatcgcc agagtgaaaa taaattccgc taccagcaac
     8041 caacccgcga cgaaagggct gatgaggaaa attaacgggt ttacgattaa gaaaatgatg
     8101 agggcgagtt tgtaccagtc gggggactgg cccaaaaaat tgcgccatag cgcgcggccc
     8161 caggagatct ccatgatggt ttcccttacc ttacaaataa tcaatgatgt ttttatgttt
     8221 aaacgcaaag cttaacggtc aggcaggagt gaggcaagtc ttgatagtca aggggaaaga
     8281 gatgcggaaa atgaagcctt gatccctttt tcttcttttt gtctgctatc agcgtagtta
     8341 gccctctggt atgatgagtc caactttgtt ttgctgtgtt atggaaatct cactatggtc
     8401 attaaggcgc aaagcccggc gggtttcgcg gaagagtaca ttattgaaag tatctggaat
     8461 aaccgcttcc ctcccgggac tattttgccc gcagaacgtg aactttcaga attaattggc
     8521 gtaacgcgta ctacgttacg tgaagtgtta cagcgtctgg cacgagatgg ctggttgacc
     8581 attcaacatg gcaagccgac gaaggtgaat aatttctggg aaacttccgg tttaaatatc
     8641 cttgaaacac tggcgcgact ggatcacgaa agtgtgccgc agcttattga taatttgctg
     8701 tcggtgcgta ccaatatttc cactattttt attcgcaccg cgtttcgtca gcatcccgat
     8761 aaagcgcagg aagtgctggc taccgctaat gaagtggccg atcacgccga tgcctttgcc
     8821 gagctggatt acaacatatt ccgcggcctg gcgtttgctt ccggcaaccc gatttacggt
     8881 ctgatcctta acgggatgaa agggctgtat acgcgcattg gtcgtcacta tttcgccaat
     8941 ccggaagcgc gcagtctggc gctgggcttc taccacaaac tgtcggcgtt gtgcagtgaa
     9001 ggcgcgcacg atcaggtgta cgaaacagtg cgtcgctatg ggcatgagag tggcgagatt
     9061 tggcaccgga tgcagaaaaa tctgccgggt gatttagcca ttcaggggcg ataatccctt
     9121 ccgtttaaag agcaaacccc tcaaacgagg ggttttttgt tgtttttaca gatttcccat
     9181 tcttggcggg caacgttcca gcaactcgat gctgccgtct tcgttttgct gttcgagcat
     9241 cacatcaaat ccccacaggc gatgcacatg cttcaggact tctttgcgcc cccgatccag
     9301 cggtgcgcga ttatgtggaa tataacgcag cgtcagcgaa cggtcgccgc gcaaatccac
     9361 gttccagatc tgaatattcg gctccagatt acttaagtta tattgcgacg ataaccggtt
     9421 acggatctcc cgataacctt cttcattatg aatagcggaa atctccagat aattatgccg
     9481 atcatcatcc tgcacggtga agaagcggaa atcacgcatc actttcggtg acagaaactg
     9541 gctgataaaa ctctcatctt tgaaatcacg catcgcgaaa tgcagcgttt ccagccagtc
     9601 ggaaccggcg atatccggga accagtattt gtcttcttcc gttggcgact gacaaatccg
     9661 tttaatatcc tggaacatgg cgaacccgag ggcatacggg ttgatgccgc tgtaccacgg
     9721 gctgttatag gggggcagga agaccacatt ggtgtggctg tgcaaaaact ccagcataaa
     9781 acgttccgtt actttccctt catcatacag atggttaagg atggtatagt gccagaaggt
     9841 cgcccagcct tcgttcatca cctgagtctg tttttgcgga taaaaatact ggctcacctt
     9901 acgcacaata cgcaggattt cacgctgcca tgattccagc agtggcgcat ttttctccat
     9961 aaaatagagc aggttttctt gtggttcgga cggatagcgg cgcgcttcag caaccgtttt
    10021 ctcttcctcg cgcttcggca gggtacgcca gagcatattg acctgacttt gtagatactc
    10081 ttcgcgactt ttctgccggg ctttctcttc ttgcagcgag attttttgcg ggcgtttgta
    10141 ccggtccacg ccgtagttca tcagcgcgtg gcacgagtcc agaagccgtt ctacttcatc
    10201 aacgccataa cgttcttcgc actcggtaat atatttacgg gcaaaaatca gataatcgac
    10261 aatcgaactg gcgtcggtcc aactgcggaa taagcaattg tttttaaaga aagagttatg
    10321 cccatagcag gcgtgtgcca tcaccagcgc ctgcatggta atggtgttct cttccatcaa
    10381 gtaagcgata cacgggttag agttaatgac gatttcatag gccagccctt gctgaccgtg
    10441 cttatacagc cgttcagtct cgataaactt tttaccgaat gaccagtgcg gatagttaat
    10501 tggcatgccg acgctggaat aggcatccat catctgttct gaggttatca cttcaatctg
    10561 gtgcgggtag gtatccagcc ggtagagttt cgccacccgg tctatctctg ccagataaac
    10621 atccagcagg tcgaacgtcc agtcgggtcc atcgctcaaa cgtgtggtgt ccttattcat
    10681 agaatcgatc gtcgccatac gcgcacctca ttgttgtcgg cgctctctgt gtggagcacc
    10741 tcatttcaag catagaacac ctgttaaaaa ccgcgtcgcc ggagaatttt tttctttgcg
    10801 atttcttatt atcagagtgc cactaatccg cttctgaacg gaattttatg ctggataaaa
    10861 agggcgttca gcaggagata ctaaagacgc catattgccg cagagtcagg gagatgtgag
    10921 tcagctcacc ataaaaaagc cgtatgttga ataatatttt caactgagtt atcaagatgt
    10981 aattagatta ttattctttt actgtatcta ccgttatcgg agtggctatg cgagttgtca
    11041 tactgggaag tggtgtggta ggcgttgcca gcgcctggta cttaaatcag gcaggacatg
    11101 aggtcactgt cattgatcgg gagccggggg ccgccctgga aaccagtgcc gcgaatgccg
    11161 ggcaaatctc ccccggatat gctgcaccgt gggcagcacc aggtgtgcct ttaaaagcga
    11221 ttaaatggat gttccagcgc catgcgccgc tggcggttcg tctcgacggt acgcagtttc
    11281 agttgaaatg gatgtggcaa atgttacgta actgcgacac cagccactac atggaaaaca
    11341 aagggcggat ggtgcgtctg gcggaataca gccgtgattg cctgaaagca ttgcgcgccg
    11401 aaaccaatat tcagtatgaa gggcgtcagg gtgggacgct gcaactgttc cgtaccgaac
    11461 aacagtatga aaatgcgacc cgcgatatcg ccgtgctgga agatgccggc gtaccgtatc
    11521 agctgctgga atccagccgc ctggcggaag tggatcccgc gctggcagaa gtagcgcaca
    11581 aactgacggg cggcctgcag ttacccaatg atgaaaccgg agactgccag ctgtttaccc
    11641 agaatctggt gcggatggca gagcaggcgg gggttaaatt ccgctttaat acgcccgttg
    11701 accaactgct ttgcgacggc gagcaaatct acggcgtgaa gttttgcgat gaagtgatta
    11761 aggccgatgc gtatgtgatg gcgtttggtt cttactcgac ggcgatgctc aaaggcattg
    11821 ttgatattcc ggtgtatccg ctgaaaggct actcgctgac tattccaatt gcgcaggaag
    11881 atggtgcgcc agtatccacc attcttgatg aaacctacaa aatcgccatt acccgtttcg
    11941 ataaccgcat tcgtgttggc ggaatggcgg aaattgttgg ttttaacacc gagctgttgc
    12001 aaccgcgtcg tgaaacgctg gaaatggtgg ttcgcgatct ctatccacgc ggcggtcatg
    12061 tcgagcaggc gactttctgg acgggtttgc gcccgatgac gccagacggt acgccggttg
    12121 tcgggcgtac acgctttaaa aatctgtggc tgaataccgg tcacggcacg ctcggctgga
    12181 cgatggcttg cggttccggt cagttgttaa gcgatctgct ctctggtcgt acgccagcga
    12241 tcccatatga ggatctaagc gtagcgcgct acagccgtgg atttacgcca tcacgtccgg
    12301 gccatttaca tggcgcacac agctaaggaa acgagatgac ccgtccgata caggccagcc
    12361 tcgatctgca ggcattaaaa cagaatctgt ccattgtccg ccaggccgcg ccgcacgcgc
    12421 gcgtctggtc ggtggtaaaa gcgaacgctt acgggcatgg tattgagcgt atctggagcg
    12481 cgctcggggc caccgatggc tttgcattac ttaacctgga agaggcaata acgttacgtg
    12541 agcgcggctg gaaggggccg atcctgatgc tggaaggatt tttccatgct caggatctgg
    12601 agatttatga ccagcaccgc ctgaccacct gcgtacacag caactggcag ctcaaagcac
    12661 cgttggatat ttatcttaaa gtgaacagtg ggatgaatcg gttgggcttc cagcccgatc
    12721 gcgtgcttac cgtctggcag cagttgcggg caatggcgaa tgttggcgaa atgaccctga
    12781 tgtcgcattt tgctgaagcg gaacatcctg atggaatttc cggcgcgatg gcgcgtattg
    12841 agcaggcggc ggaggggctg gagtgtcggc gttcgttgtc caattcggcg gcgactctgt
    12901 ggcacccgga agcgcatttt gactgggttc ggcctggcat tattttgtat ggcgcttcgc
    12961 cgtccggtca gtggcgtgat atcgccaata ccggattacg tccggtgatg acgctaagca
    13021 gtgagattat tggtgtccag acgctaaaag cgggcgagcg tgtgggctac ggcggtcgct
    13081 atactgcgcg cgatgaacag cgaatcggca ttgtcgccgc aggatacgcc gacggttatc
    13141 tgcg
//
LOCUS       DBKEKI010000109        13111 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.109, whole genome shotgun sequence.
ACCESSION   DBKEKI010000109 DBKEKI010000000
VERSION     DBKEKI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.109"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..149
                     /locus_tag="ACUQPX_003092"
                     /pseudo
     CDS             1..149
                     /locus_tag="ACUQPX_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005163699.1"
                     /note="catalyzes the formation of glutamate from
                     glutamine; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="ACUQPX_003093"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="ACUQPX_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HHV9057720.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="ACUQPX_003094"
     CDS             1828..3147
                     /locus_tag="ACUQPX_003094"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HHV9057721.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3221..3649
                     /gene="tqsA"
                     /locus_tag="ACUQPX_003095"
                     /pseudo
     CDS             3221..3649
                     /gene="tqsA"
                     /locus_tag="ACUQPX_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(3674..5062)
                     /gene="pntB"
                     /locus_tag="ACUQPX_003096"
     CDS             complement(3674..5062)
                     /gene="pntB"
                     /locus_tag="ACUQPX_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HHV9057722.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFGRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5073..6605)
                     /gene="pntA"
                     /locus_tag="ACUQPX_003097"
     CDS             complement(5073..6605)
                     /gene="pntA"
                     /locus_tag="ACUQPX_003097"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HHV9057723.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQSQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7129..8073
                     /gene="ydgH"
                     /locus_tag="ACUQPX_003098"
     CDS             7129..8073
                     /gene="ydgH"
                     /locus_tag="ACUQPX_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HHV9057724.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            8259..9641
                     /locus_tag="ACUQPX_003099"
     CDS             8259..9641
                     /locus_tag="ACUQPX_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHV9057725.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVSVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9678..10400
                     /gene="folM"
                     /locus_tag="ACUQPX_003100"
     CDS             9678..10400
                     /gene="folM"
                     /locus_tag="ACUQPX_003100"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HHV9057726.1"
                     /translation="MDKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10397..10732)
                     /locus_tag="ACUQPX_003101"
     CDS             complement(10397..10732)
                     /locus_tag="ACUQPX_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HHV9057727.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            10861..11604
                     /gene="rstA"
                     /locus_tag="ACUQPX_003102"
     CDS             10861..11604
                     /gene="rstA"
                     /locus_tag="ACUQPX_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HHV9057728.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            11829..>13111
                     /locus_tag="ACUQPX_003103"
     CDS             11829..>13111
                     /locus_tag="ACUQPX_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylate symporter family
                     transporter"
                     /protein_id="HHV9057729.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGGGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSMMSGVISARLLKD"
BASE COUNT         3218 a         3305 c         3156 g         3432 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc tgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cttggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccacca
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatca tcaccggcct
     3241 ggtcgcctgg gcgatgctcg ccgcactcga tgttcgcttc gcttttgtct ggggattgct
     3301 ggcctttgcg cttaattaca tccctaatat tggttcagtt ctcgcggcaa tcccccctat
     3361 cgctcaggta ctggtgttta atggcttcta cgaagcgttg ctggtgctgg cgggatatct
     3421 gctgattaat ctggtcttcg gcaatattct ggagccgcgc atcatgggac gtgggctggg
     3481 gctttcgaca ttggtggtat ttttgtcgtt gattttttgg ggatggttgt taggaccggt
     3541 gggtatgctg ctttccgtgc cgttgacaat tattgtcaaa attgcgcttg aacaaacagc
     3601 gggaggtcaa agcatcgccg ttctgttaag cgatctcaat aaagagtgac ggcctccgca
     3661 gaggccgtca gggttacaga gctttcagga ttgcatccac gctggctttg gcgtcaccaa
     3721 acagcatgtg ggtgttttcc ttgaagaaca gcgggttttg cacaccagca tagccagtgt
     3781 tcatcgaacg tttaaagaca atcacgttct gcgctttcca cacttccagc acaggcatac
     3841 cagcaatcgg actcttcgga tcatcctgcg ccgccgggtt aaccgtatcg ttagcaccaa
     3901 tcaccagtac ggtatcggta tcagcaaagt catcgttgat ctcgtccatt tccagcacga
     3961 tgtcatacgg tacttttgct tcagccagca atacgttcat atgtccaggc aaacgccccg
     4021 caaccgggtg gataccgaaa cgcacattaa taccacgagc gcggagtttc tcggtaattt
     4081 cagcgacagg atattgcgcc tgcgcgactg ccatgccgta ccccggagta atgatcactg
     4141 aatgggagtt tttcagcagt tccgctgtct cttctgcggt gatttcgcgg tgctcaccca
     4201 cttcctgatc atcgccagta gaagagccgt cggtgccgaa accacccgca ataacgctga
     4261 taaaggaacg gttcatcgcc ttacacataa tgtaagaaag gatagccccc gaagaaccga
     4321 ccagcgcacc ggtcacaatc agcaggtcgt tgctgagcat aaagcccgca gccgcagccg
     4381 cccagccgga gtacgagttc agcatcgaca ccaccaccgg catatctgca ccaccgatgg
     4441 aggcgactaa atgccagccg aataccagcg caatggcggt cattatcagc aatgccagca
     4501 cttgcaggcc gacgctgtcc gtgcgaccaa atacaatcag cagcaagaag gaaacgacca
     4561 gagccgccag gttcattttg tgacggtttg gcagcatcaa cggtttagac gaaatcttgc
     4621 cacacagttt gccgaacgcc accaccgaac ccgtgaacgt taccgccccg atgaagatac
     4681 cgaggaacac ttccgtcagg tgaatattga ccagaatcgg tgccattccc gcgtcatgat
     4741 gcagataact gttaaagcca accagcactg ccgccagacc cacgaagctg tgcaggatcg
     4801 ccaccagttc tggcatttcg gtcatttcaa ctttcttcgc cagccggata ccaattgccc
     4861 caccaatgac catcgccagc aagatccagc caacattacc cgtatccggt ccaaaaatgg
     4921 ttgcgattaa cgcaatcgcc atcccggcga taccgaagtt gttaccctgg cgagacgttt
     4981 catgtttcga aagaccggcc agactgaaga taaacaggat cgcggcaaca atgtatgcag
     5041 ctgtaactaa tcctccagac atatgttacc ccttaatttt tgcggaacat tttcagcatg
     5101 cgctgagtca cggtgaagcc accgaaaata ttaatgctgg ctataagcac cgcgataaaa
     5161 ctaaggaagc taacccagcc gccctggcca atctgcaaca gtgctccgac aacaataatc
     5221 cctgaaatcg cgttggtaac cgacatcaac ggtgtatgca gcgcgtgcga tacattccac
     5281 accacgtaat aaccgacaac gcaggccagc gcgaaaacgg tgaagtgccc aagaaactct
     5341 ttcggcgcaa cgcttgacag ccaaccgaac aggatgattg ccagcgccat caacgcgtat
     5401 ttacgccacg gtgagcaggc acatttttcc tcagttttca cttccggtgc cgctttttgt
     5461 gccgcctgcg actgagctga tacctgaatc ggcggtgccg gccaggtaat ttcgcccgca
     5521 cggatcacgg tcacgccgcg aatcaccaca tcatcaaaat caacagtgat attgccgtct
     5581 ttctctttgc acaacagttt cagcagatta acgaggttag taccgtaaag ctgtgaggat
     5641 tgcgtcggca gacggcccgg aagatcggta taaccaatca ctttgacacc attttccgta
     5701 ctgaagattt cacccggcac ggtgtattca cagttgccac cgttttgggc tgccaggtcg
     5761 acaatcacac tgcccgcctt catggagtca accatttcac gggtaattag cttcggcgct
     5821 ggtttgcctg gaataagcgc ggtggtgaca atgatatcga cctcttttgc ctgggcggca
     5881 aagagttcca tttccgcttt gatgaacgcg tccgacatca ctttggcata gccatcgccg
     5941 ctgcccgctt cctctttaaa atccagctcg aggaattccg cgcccatact ttgaacttgt
     6001 tctttcactt ccgggcgggt gtcgaatgca cgcacaatcg cgccgagact gtttgctgcg
     6061 ccaatggcgg ccagacctgc aacaccggca ccaatcacca tcacttttgc cggtggcact
     6121 ttcccggccg cagtgatttg cccggtaaag aagcgcccaa attcatgtgc cgcttcaaca
     6181 atggcgcgat aaccggcgat attcgccatc gagcttagtg cgtccagcga ttgtgcgcgt
     6241 gagatacgcg gcacagagtc catcgccatc acggtcacgt tacgttccgc aagtttttgc
     6301 attaattccg gattctgcgc aggccagata aaactcacca gcgttgtccc gggattcagt
     6361 aacgcaattt catcatctaa cggcgcattg accttcagaa tgatctctga ctgccagacg
     6421 ctattccctt ctacaatttc agcgcccgct tgcacaaacg ctttatcgtc aaaacttgcc
     6481 agttgacccg cgccgctctc taccgcgacg gtaaaaccca gtttcagcag ctgttccact
     6541 gtttttggcg ttgctgcaac acgggtttca ttggttaacc gttctcttgg tatgccaatt
     6601 cgcatgatat tcccttccat cggttttatt gatgatggtt tgcctgtgtc aggagccaca
     6661 caagccgctc atgtacgagc taaatgttac tccgttaaaa taaattagta acaaacgccc
     6721 tataacgtac tgaaaattat gcctgtgatc tagcgccaaa aatcagtatt tcggcgtgaa
     6781 ctcgcaaaat attaacgatt cagccgtgat agtgggataa acaccttaga acgccggata
     6841 aagactgata attgtcttcg acggtcgggt aaaacgagac atcgccccag cacgaatcac
     6901 tacttaacat taaattaact tatacaattc agttgcttca gtagtaatga tgctgatacg
     6961 gctgtttttt aagcatagac ggtcatttga gcaggattaa aattggttta aggaatgtga
     7021 tatgaaaaat gacgcagaca gttacaccgt ttaaatgcaa taatcagcca cgtttctcgt
     7081 taataacaat accagtacct ggtttgcgca aggcgaagga ttatttttat gaagcttaag
     7141 aacaccctcc tggcgtcggc actgctttct gctacggcat tctccgttaa cgcagcaaca
     7201 gaactgacac cggagcaagc ggcagcggtt aaaccttttg accgtgtagt ggttaccggt
     7261 cgttttaatg ctattggcga agcggtgaaa gccgtttctc gtcgcgcaga taaagaaggt
     7321 gccgcctctt tttatgttgt cgacacttct gattttggta acagtggtaa ctggcgtgtg
     7381 gtcgctgacc tctataaagc cgatgctgaa aaagcagaag aaacaagtaa tcgcgtaatt
     7441 aacggtgttg tcgaactgcc gaaagatcag gctgttctga ttgaaccgtt tgacacggtc
     7501 accgtccagg gcttctatcg tagccagcca gaagtcaatg atgccatcac caaagcggca
     7561 aaagcgaaag gtgcctactc tttctacatc gttcgtcaaa tcgatgccaa ccagggcggc
     7621 aaccagcgta ttactgcatt catctataaa aaagatgcta agaaacgtat cgtccagagc
     7681 ccggatgtga tcccggcaga ttccgaagca ggacgtgcag ctctggctgc cggtggcgaa
     7741 gccgcgaaga aagttgagat cccgggtgtt gcgactaccg catcaccaag ttctgaagtc
     7801 ggtcgcttct ttgaaaccca gtcatcaaaa ggcgggcgtt acaccgtcac gctcccggat
     7861 ggcactaaag tcgaagaact gaacaaagcg acagcagcga tgatggtccc gttcgacagc
     7921 atcaaattct ctggcaacta cggcaacatg accgaagtct cctatcaggt tgcgaaacgt
     7981 gccgcgaaga aaggtgctaa gtattaccac atcacccgcc agtggcagaa acgtggtaat
     8041 aacctgaccg tcagcgcaga tctgtataaa taactattca gttactccta taaggcggct
     8101 tgatgccgcc tttcgcattt ttagtcacaa atttccccgc gaccattgca tgcagacaca
     8161 tctcttcgta aaatcccgcg cttcagtggc aaactccatt tttatgcgtt ttgccctaat
     8221 atttattctt cttatcacgt tttaatcact ggatatcgat ggaaaagaaa ctgggactga
     8281 gcgcactcac cgcgctggta ttaagctcaa tgctgggcgc gggtgttttc agtctgccgc
     8341 aaaatatggc ggcagttgcc agcccggcag cactgctcat cggctggggt attactggcg
     8401 ctggcatttt attgctggcc tttgccatgc tgatcctcac gcgcattcgt cccgaacttg
     8461 atggcggtat ctttacctat gcccgcgaag ggtttggcga gctaatcggc ttttgttccg
     8521 catggggata ctggctgtgc gcggtcatcg ccaacgtctc ctatctggtg atcgtttttt
     8581 ccgcgttaag cttttttacg gacacgccgg aattgcgcct gtttggcgat ggtaatacct
     8641 ggcaatcgat agtcggtgca tcggctttat tgtggattgt tcactttttg atcctgcgcg
     8701 gggtgcaaac cgctgccagc attaacctgg tggcgacact ggcaaaattg ttgccgctgg
     8761 gtctgtttgt tgtgctggcg atgatgatgt tcaaactgga taccttcaag ctcgacttca
     8821 ccggacttgc acttggcgta tctgtttggg aacaggtgaa aaacaccatg ctgatcaccc
     8881 tgtgggtttt cattggtgtg gaaggtgccg tggttgtttc tgcgcgtgcg cgtaataaac
     8941 gtgatgttgg caaagcgaca ctgctggcgg ttctctccgc tctgggcgtt tacttgctgg
     9001 taacgctgct ttcgctgggt gtggtggcac gtcctgaact ggctgaaatt cgtaacccgt
     9061 caatggccgg tctgatggtg gaaatgatgg gaccatgggg cgaaatcatc atcgctgccg
     9121 gtttgatcgt ttccgtttgc ggcgcgtact taagctggac catcatggcg gcagaagttc
     9181 cgttcctggc tgccactcat aaagcattcc cacgcatttt tgcgcgccag aacgcacaag
     9241 ctgcgccatc cgcctcactg tggttgacca acatctgcgt gcaaatttgt ctggtactca
     9301 tctggctgac cggttccgat tacaacacgt tgctgaccat cgcctcagaa atgattctgg
     9361 tgccctattt cctggtcggc gcgttcttgc tgaaaatcgc aacccgtcca ctgcataaag
     9421 cggtaggtgt cggtgcctgc atttatggct tatggttatt gtacgcctct ggtccaatgc
     9481 acttgctgtt atccgttgtt ctgtatgcac cggggctgct ggttttcctg tatgcgcgca
     9541 aaacgcatac acatgataat gtactgaacc gtcaggaaat ggtgctgatt ggcatgctgt
     9601 taattgcctc agttccggcg acctggatgc tggtgggata acgttcatcc catcgttgga
     9661 taacaaagga gataacgatg gataaaaccc agcccttgcc aatattaatt actggcggag
     9721 gtcgtcgcat cggcctcgcc ctcgcatggc atttcattaa tcaaaagcaa ccggtgattg
     9781 tcagctatcg gacacactat ccagccattg atggactgat taatgcaggt gcgcagtgta
     9841 ttcaggctga tttttcgacc aacgacggtg tgatggcgtt tgccgatgaa gtactaaaaa
     9901 ccacccatgg tctgcgtgct attttgcata acgccagtgc gtggattgcg gaaaaaccgg
     9961 gtgcgccact ggccgacgta ctggcttgca tgatgcagat ccacgttaat accccatacc
    10021 tgctcaacca tgcgctggaa agattactgc gtgggcacgg acacgccgcc agcgatatca
    10081 ttcactttac cgattatgtg gtggagcgcg gtagcgacaa acatgttgcg tatgctgcaa
    10141 gcaaagcggc actggataat atgacccgct cgtttgcccg caagctggca ccggaagtga
    10201 aagtgaattc tattgcgcca tcgctgatcc tgtttaatga acatgatgat gccgaatatc
    10261 gacaacaggc gctgaataaa tcactgatga aaaccgcgcc tggcgagaaa gaagtgatcg
    10321 acctggtcga ttacttactt accagttgct ttgtcaccgg acgcagtttc ccacttgatg
    10381 gcggtcgtca tctgcgttaa tgcagcttta tccagcatat aatcaacacc catgcgctta
    10441 tcccccaaca cgcaacagaa ccaacaaaag cggcgggcaa gcgcatcatt ccggtgaaat
    10501 accacagcga caccaggtaa acaaaatagg gaataatcga ccacatacta aaaatgatgg
    10561 ttgcgcgtaa ggcttcaatg ccgcgttcgc tggcaacaat ataatgcgcg ataagcgcaa
    10621 aggtcgggaa aagtggaatc agcccggcga tataataatt tttcgttttt gctaaaacac
    10681 caatcaacag cactaccagc gcaccgagcg cggctttgat taccagcccc atctttttac
    10741 cttaacactt ccataacaag tcatcagtag aatacctgat gaaaacttgt ttagaaacga
    10801 ttgatagtaa gtaaaaacag cgcgatgtat tgtgacgttt ttatatctac cgtgaatgtt
    10861 atgaacacta tcgtatttgt ggaagatgat gcggaagtcg gttcactgat tgccgcgtac
    10921 ctggcaaaac atgatatgca ggttaccgta gaaccgcgcg gcgaccaggc cgaagaaacc
    10981 attttgcgag aaaatccgga tttggtgtta ctcgacatca tgctaccagg caaggacggc
    11041 atgaccattt gtcgtgattt acgcgcaaag tggtctggac cgattgttct tctaacctct
    11101 ctcgatagcg atatgaacca catcctggca ctggaaatgg gtgcctgcga ctatattctc
    11161 aaaacgacgc cccctgctgt tttgctagcg cgtttacgtt tgcatttgcg tcagaatgag
    11221 caagccacac tgaccaaagg tcttcaggaa acgtctctga ctccctacaa agccctgcat
    11281 ttcggcacgt taactatcga ccccatcaac cgcgtagtca ccttggctaa cactgaaatc
    11341 tcgctctcga cagctgattt cgaattattg tgggaactag ctacgcatgc cgggcaaatc
    11401 atggaccgcg atgcattgct gaaaaattta cgcggcgtca gttatgacgg actggatcgt
    11461 agcgtggacg tggctatttc gcggttaaga aaaaaactgc tcgataacgc cgcagaacct
    11521 tatcgcatta aaactgtgcg taacaaaggc tatctttttg cgcctcatgc atgggatagt
    11581 gacaataaac aatcagatga ctaatatttg ttcatctgaa aaagagaaca attaacgaat
    11641 tcaattaata agagcttatt caaattccaa accccctaca tttgatatga atatgagaaa
    11701 aaatttctct ataatcgcct cgtccctgcg tcttatggcg cagatttttt acaacaaact
    11761 ctttccggca tttcaggtgc tgaatgaggc aagaggctta aaaaacggtt acaactttca
    11821 catatattgt gctttttttg atctgctcat gggggatttt caaaacggat caaaagagcg
    11881 aatccctttc caaaagtgtt tttatcgcga ttttcctggg ggctatcatt ggtatctccc
    11941 tgcatttcat tcctgagaca agcagtgaaa ccgttatcga atggtataat attgctggta
    12001 acggttatgt taatttatta aaacttgttg ccgttccact catattcatt tcgattcttt
    12061 cagcgattaa taagctcgaa aatagcgctg gtattggcaa gatgtcttta actattgttg
    12121 cctgtatgtt gtgtttagtc atgattgcag gtttagttgg tctggctatt gccagtgcaa
    12181 ttggccttga tgccagcgcc tttggtcata tgcaatcctc tttaaccagc gatgacgtct
    12241 cgaaaacagc tgcggtatct ttaccgcgac tcattacgtc attgatccca accaacctct
    12301 tccttgatct gaccggggca agaagtgttt cggttattgg catcgtcatc tttacaattc
    12361 ttgctggtat tgcgctgtta aaagtaaaga aagatgcacc agcagagggc gaaaaattga
    12421 gcgcggggat caacgcgatt caaatatggg taatgaaaat ggtccgtatt gtcattgcgc
    12481 tgacgcctta tggggtaatg gcactaatgg cgaaagtctt ctcaacgtat cagctttcac
    12541 agtttaccaa tttgctcggg ttcatcgtcg cctgttatct ggccgttttt gtgatgttta
    12601 ttgttcactc gggcattttg ctgttaatag gtaaaaaccc cgttaactat tacaagaccg
    12661 tctggcaagt acttaccttt gcgtttgttt ctcgcagtag tgcggcttca attcctctgg
    12721 ctatttctgc attggaaaaa ttgggggttc caaatacgat tgctaatatt gcagcgtctt
    12781 ttggttccag catgggacaa aatggctgcg ctgggattta tcctgccgtg atggtggcga
    12841 tgattgctcc aacaatgggt atcgatccct tctcattgca attcctcgca actcttttac
    12901 ctgccatcgc gcttggctca ataggggtag caggcgtagg tggtggtggg acattcgcgg
    12961 cgctgattgt cctttcaaca cttaatttcc ctgtcgcatt agtagttatt tttattgcca
    13021 tagagccgat cgttgatatg gcaagaacag cgcttaatgt gaatggttca atgatgtccg
    13081 gtgtaatatc agcccgtctt cttaaggata g
//
LOCUS       DBKEKI010000110        12976 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.110, whole genome shotgun sequence.
ACCESSION   DBKEKI010000110 DBKEKI010000000
VERSION     DBKEKI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.110"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..159
                     /locus_tag="ACUQPX_003104"
     CDS             <1..159
                     /locus_tag="ACUQPX_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HHV9057730.1"
                     /translation="ETYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKN
                     GEWNYIGK"
     gene            complement(300..1073)
                     /gene="uxuR"
                     /locus_tag="ACUQPX_003105"
     CDS             complement(300..1073)
                     /gene="uxuR"
                     /locus_tag="ACUQPX_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HHV9057731.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1288..2748)
                     /locus_tag="ACUQPX_003106"
     CDS             complement(1288..2748)
                     /locus_tag="ACUQPX_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HHV9057732.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2829..4013)
                     /gene="uxuA"
                     /locus_tag="ACUQPX_003107"
     CDS             complement(2829..4013)
                     /gene="uxuA"
                     /locus_tag="ACUQPX_003107"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HHV9057733.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRLGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4353..5696
                     /gene="gntP"
                     /locus_tag="ACUQPX_003108"
     CDS             4353..5696
                     /gene="gntP"
                     /locus_tag="ACUQPX_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HHV9057734.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGMLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5947..6849)
                     /gene="fimH"
                     /locus_tag="ACUQPX_003109"
     CDS             complement(5947..6849)
                     /gene="fimH"
                     /locus_tag="ACUQPX_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HHV9057735.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6869..7312)
                     /locus_tag="ACUQPX_003110"
     CDS             complement(6869..7312)
                     /locus_tag="ACUQPX_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9057736.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(7385..7915)
                     /gene="fimF"
                     /locus_tag="ACUQPX_003111"
     CDS             complement(7385..7915)
                     /gene="fimF"
                     /locus_tag="ACUQPX_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HHV9057737.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7925..10561)
                     /gene="fimD"
                     /locus_tag="ACUQPX_003112"
                     /pseudo
     CDS             complement(7925..10561)
                     /gene="fimD"
                     /locus_tag="ACUQPX_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
     gene            complement(10628..11353)
                     /gene="fimC"
                     /locus_tag="ACUQPX_003113"
     CDS             complement(10628..11353)
                     /gene="fimC"
                     /locus_tag="ACUQPX_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HHV9057738.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11390..11929)
                     /locus_tag="ACUQPX_003114"
     CDS             complement(11390..11929)
                     /locus_tag="ACUQPX_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9057739.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11994..12542)
                     /gene="fimA"
                     /locus_tag="ACUQPX_003115"
     CDS             complement(11994..12542)
                     /gene="fimA"
                     /locus_tag="ACUQPX_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HHV9057740.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3117 a         3362 c         3153 g         3344 t
ORIGIN      
        1 gaaacctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
       61 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
      121 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
      181 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
      241 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
      301 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
      361 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
      421 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
      481 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
      541 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
      601 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
      661 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
      721 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
      781 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
      841 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
      901 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
      961 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     1021 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     1081 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     1141 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     1201 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     1261 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     1321 ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag
     1381 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     1441 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     1501 ctgctcatcc acgccctggg tgtaacgcat ccagccagcc acgcccagcg ccaggtgacg
     1561 ccagctgccg ccgttttgca agtgcagacg caccgggtcc agtagacgct gcggtaactt
     1621 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     1681 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     1741 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     1801 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     1861 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgcgcc
     1921 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     1981 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     2041 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     2101 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     2161 cttcgccaca tgaccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     2221 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     2281 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     2341 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     2401 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     2461 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     2521 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     2581 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     2641 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     2701 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     2761 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc
     2821 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     2881 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     2941 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     3001 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     3061 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     3121 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     3181 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     3241 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     3301 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     3361 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     3421 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     3481 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     3541 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctagacgttt caggatatgc
     3601 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     3661 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     3721 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     3781 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     3841 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     3901 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     3961 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     4021 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     4081 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     4141 ctaccttttg gtaagacatg tcaacctgaa ttctgaaatt ggttaaccac atcacaagaa
     4201 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     4261 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     4321 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     4381 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     4441 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     4501 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     4561 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     4621 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     4681 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     4741 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     4801 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     4861 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     4921 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     4981 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     5041 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     5101 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     5161 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     5221 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     5281 gtgcgggtgg catgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     5341 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     5401 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     5461 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     5521 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     5581 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     5641 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     5701 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     5761 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     5821 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     5881 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     5941 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     6001 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     6061 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     6121 gccaacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     6181 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     6241 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     6301 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     6361 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     6421 agctttaatc gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     6481 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     6541 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     6601 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     6661 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     6721 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     6781 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     6841 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     6901 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     6961 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     7021 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     7081 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     7141 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     7201 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     7261 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     7321 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     7381 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     7441 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     7501 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     7561 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     7621 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     7681 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     7741 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     7801 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     7861 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     7921 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     7981 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     8041 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     8101 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     8161 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     8221 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     8281 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     8341 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     8401 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     8461 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     8521 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     8581 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     8641 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     8701 agctggcact ggcatgtcac cactgagatt tactgtcaga acgcagccag tggctgaaag
     8761 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     8821 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     8881 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
     8941 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
     9001 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
     9061 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
     9121 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
     9181 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
     9241 tatccacaga cagagcgccc agtgccccca tgtttttccc gataccgaaa ttaaaagcac
     9301 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
     9361 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
     9421 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
     9481 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
     9541 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
     9601 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
     9661 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
     9721 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
     9781 gcagggaacg taaaggaatt atgcctcgct caatccaggt aatgatatgc tgccatttat
     9841 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
     9901 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
     9961 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
    10021 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
    10081 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcctggacca
    10141 ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg
    10201 tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
    10261 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    10321 cgacgcgata cgtccctggc ggtaattctt gcccatttta aaaacgcgat aaatcggcca
    10381 cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag
    10441 gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat
    10501 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    10561 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc
    10621 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    10681 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    10741 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    10801 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    10861 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
    10921 tgataattgc gagctgtagc gtattctcag tcaattttga tttatccatt gacggaatcg
    10981 ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
    11041 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
    11101 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac
    11161 tattttcatc attatttgtc acggcaagtt gcacttgttt ttgccctgcc ggataaatta
    11221 cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga
    11281 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    11341 ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg
    11401 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    11461 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg
    11521 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg
    11581 aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac
    11641 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    11701 caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc
    11761 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    11821 cgccctgaaa ttgcatattt ccgccgggaa gcgtggtatt ccatttattt ccggccagag
    11881 caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta
    11941 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga
    12001 tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca
    12061 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    12121 aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg cacgccaacg
    12181 tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca
    12241 attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc
    12301 agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta
    12361 cgaacctgtc ccaactgaac agtttgatca acagagcctg catcaactgc gcaagcggcg
    12421 ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga
    12481 gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
    12541 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
    12601 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
    12661 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
    12721 ttggcttaat attctattgt tatctttatt tatagatttt tatattgcat gaggtggttt
    12781 ttggagagaa gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg
    12841 gaggtgatgt gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt
    12901 tttgtcgttt tttaatattt ttatgcttga gaaaaaatac gtaacttatt tatgatatgg
    12961 acagtttggc cccaat
//
LOCUS       DBKEKI010000111        12947 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.111, whole genome shotgun sequence.
ACCESSION   DBKEKI010000111 DBKEKI010000000
VERSION     DBKEKI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.111"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..178
                     /locus_tag="ACUQPX_003116"
     CDS             <1..178
                     /locus_tag="ACUQPX_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHV9057741.1"
                     /translation="LDPSRDRSTGGCGLGLAIVHSIALAMGGTVNCDTSELGGARFSF
                     SWPLWHNIPQFTSA"
     gene            254..1183
                     /gene="tus"
                     /locus_tag="ACUQPX_003117"
     CDS             254..1183
                     /gene="tus"
                     /locus_tag="ACUQPX_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HHV9057742.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(1180..2583)
                     /gene="fumC"
                     /locus_tag="ACUQPX_003118"
     CDS             complement(1180..2583)
                     /gene="fumC"
                     /locus_tag="ACUQPX_003118"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HHV9057743.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(2726..4372)
                     /gene="fumB"
                     /locus_tag="ACUQPX_003119"
     CDS             complement(2726..4372)
                     /gene="fumB"
                     /locus_tag="ACUQPX_003119"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HHV9057744.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            4571..5746
                     /gene="manA"
                     /locus_tag="ACUQPX_003120"
     CDS             4571..5746
                     /gene="manA"
                     /locus_tag="ACUQPX_003120"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HHV9057745.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            5847..7355
                     /locus_tag="ACUQPX_003121"
     CDS             5847..7355
                     /locus_tag="ACUQPX_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HHV9057746.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7581..8846)
                     /gene="uidC"
                     /locus_tag="ACUQPX_003122"
                     /pseudo
     CDS             complement(7581..8846)
                     /gene="uidC"
                     /locus_tag="ACUQPX_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(8885..10064)
                     /gene="uidB"
                     /locus_tag="ACUQPX_003123"
                     /pseudo
     CDS             complement(8885..10064)
                     /gene="uidB"
                     /locus_tag="ACUQPX_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(10061..11872)
                     /gene="uidA"
                     /locus_tag="ACUQPX_003124"
     CDS             complement(10061..11872)
                     /gene="uidA"
                     /locus_tag="ACUQPX_003124"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HHV9057747.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVVLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAPLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(12260..12850)
                     /gene="uidR"
                     /locus_tag="ACUQPX_003125"
     CDS             complement(12260..12850)
                     /gene="uidR"
                     /locus_tag="ACUQPX_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HHV9057748.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
BASE COUNT         3256 a         3385 c         3073 g         3233 t
ORIGIN      
        1 cctcgatccc agccgggatc gctcaaccgg cggctgcggg ctggggctgg caattgtcca
       61 ctctatagca ctggcaatgg gcggtacggt taattgtgac accagcgaac tgggtggtgc
      121 tcgcttctcg tttagctggc cgttatggca taacatcccg caatttacct ctgcctgaca
      181 ctacgcgcac gatggtcaag tcaccacgac tgtgctataa aataagtatg ttgtaactaa
      241 agtggttaat attatggcgc gttacgatct cgtagaccga ctcaacacta cctttcgcca
      301 gatggaacaa gagctggcta catttgccgc tcatcttgag caacacaagc tattggttgc
      361 ccgcgtgttc tctttgccgg aggtaaaaaa agaggatgag cataatccgc ttaatcgtat
      421 tgaggtaaaa caacatctcg gcaacgacgc gcagtcgctg gcgttgcgtc atttccgcca
      481 tttatttatt caacaacagt ccgaaaatcg cagcagcaag gccgctgtcc gtctgcctgg
      541 cgtgttgtgt taccaggtcg ataacctttc gcaagcagcg ttggtcagtc atattcagca
      601 catcaataaa ctcaagacca cgttcgagca tatcgtcacg gttgaatcag aactccccac
      661 cgcggcacgt tttgaatggg tgcatcgtca tttgccgggg ctgatcaccc ttaatgctta
      721 ccgcacgctc accgttctgc acgaccccgc cactttacgc tttggttggg ctaataaaca
      781 tatcattaag aatttgcatc gtgatgaagt cctggcacag ctggaaaaaa gcctgaaatc
      841 accacgcagt gtcgcaccgt ggacgcgcga ggagtggcaa agaaaactgg agcgagagta
      901 tcaggatatc gctgccctgc cacagaacgc gaagttaaaa atcaaacgtc cagtgaaggt
      961 gcagccgatt gcccgcgtct ggtacaaagg agatcaaaaa caagtccaac acgcctgccc
     1021 tacaccactg attgcactga ttaatcggga taatggcgcg ggcgtgccgg acgttggtga
     1081 gttgttaaat tacgatgccg acaatgtgca gcaccgttat aaacctcagg cgcagccgct
     1141 tcgtttgatc attccacggc tgcacctgta tgttgcagat taacgcccgg ctttcatact
     1201 gccgaccatc tgttctggcc gtacccagct gtcaaactcg gcttcgctaa gataccccag
     1261 cgcaagggcc gcagctttta aggtcagccc ttctttatgc gcttttttgg cgatctccgc
     1321 ggctttgtca taaccaatgt gggtgttaag cgcagtcacc agcatcagcg attcattgag
     1381 taattgattg attcgctcac gattcggttc aatacccact gcgcagtgtt tgttaaaact
     1441 ttccatgcca tctgccagca agcgcaccga ttgcaggaaa ttatggatca ccatcggacg
     1501 gaagacgttc agttcaaagt taccggaagc gccacccatg ttgatcgcca cgtcgttccc
     1561 cattacctga cagcagagca tggttaatgc ttcacactgg gttgggttca ctttccccgg
     1621 catgattgag ctgcccggct cattttccgg gattgagatt tcaccaattc cgcagcgcgg
     1681 gccagaggcc agccagcgga catcattggc gattttcatc agtgacgcag ccaacccttt
     1741 caacgcgccg tgcgcctgaa ccagggcatc acaggtcgca agcgcttcaa atttgttcgg
     1801 cgcggtaaca aacggtgcac aggtaatgac tgccagttca tctgctacgc gacgcgcata
     1861 ctccggatgg gtatttagtc cagtacccac cgctgtaccg ccaagagcca gttccgctac
     1921 gtgaggcagg ctgtattcga tatgtttgag attatgctcg agcatcgcta cccagccgga
     1981 aatctcctgc cctagtgtta gcggcgtggc gtcctgcaag tgggttcgac cgattttgac
     2041 gatatcggca aatgcacgcg atttttcact cagcgtctgt gtcagggttt taagctgcgg
     2101 aatgagttgc ttgcgcagtg ccagcagcgc cgcaacgtgc atcgccgtcg gaaagacatc
     2161 gttggaactt tggcttttgt tcacgtcgtc gttagggtga actttacgtt ccatcccgcg
     2221 cacaccgccg agtaattcac tggcccggtt agccagcact tcgttcatgt tcatgttact
     2281 ttgcgtgccg gagccggtct gccagatagc cagcgggaat tcgtcgtcat gctgtcctgc
     2341 cagtacttca tccgccgcct gccgaatggc gctcgctttc tcttcagaca acaagtctaa
     2401 atcttcatta acttttgccg ctgcgcgctt ggttagcgcc agcgcatgaa tcagtgaggt
     2461 gggcattttc tccgtcgaaa tgcggaaatg ctccagcgag cgttgagttt gtgcgcccca
     2521 cagcttatct gccgggacat caatcgcccc catcgaatct ttttcgctgc gtactgtatt
     2581 catggcctgc tcctcacctg attaattttt tctttctgtt ttgctttcgt taagcaactt
     2641 ttagctcact cattatttac tatttgataa caaatgtttg gtctttcgtg ccatgtaaaa
     2701 aaaccgcccc gaagggcggc tctgtttatt tcacacagcg agtgcactgt gtgagttgta
     2761 tctgctggaa gaagtcattt cctttatcat ccacaaggat aaacgccggg aaatcttcca
     2821 cttcaatttt ccagatggct tccattccca gttccggata ttcaacacat tccaggctct
     2881 taatacttcc ctgcgccaat acagcggccg gaccaccgat actgccaagg tagaagccgc
     2941 cgtgtttttt acaggcatcc gtcacctgct ggctgcggtt gcctttcgcc agcatgatca
     3001 tacttccgcc ctgcgcttgc agttgatcga cataagaatc catccgtccg gcggtcgttg
     3061 ggccaagaga accggaggca taaccttccg gcgttttggc cggacccgcg tagtaaatcg
     3121 gatgatcttt aatgtattgc ggcagccctt caccgttatc catccgctct ttcagtttgg
     3181 cgtgagcaat atcacgacca acgataatcg tgccgttaag cgataagcgt gtagaaacgg
     3241 gatactgcga caactgtgcg aggatctctt tcatcggacg gttaaggtca acgcgcaccg
     3301 cttcgccttc tcccgcttta cgcagctctt cagggatata tttacctgga ttatgttcca
     3361 gtttttcgat ccagatcccc tgacggttga tcttcgcttt gatattacgg tcagcagagc
     3421 aggaaacacc cataccaacc ggacaggatg cgccgtgacg tggcaggcga atcacgcgga
     3481 tgtcgtgagc gaagtattta ccaccaaact gcgcacccag accgagattt tgcgcttcga
     3541 tcagtaattc tttttccagt tccacatcgc ggaacgcctg accgtgctca ttcccttccg
     3601 ttggcagttc atcatagtat ttcgcggaag ccagtttcac cgttttaagg ttcgtttctg
     3661 cagaagttcc accaataacg aacgcaatat gatacggagg acaggccgcg gtacccagcg
     3721 tgcgcatctt ctcaaccagg taatttttca gtttccccgg cgtcagtaac gctttggttt
     3781 cctgatagag atacgtcttg tttgccgaac caccaccttt tgcgatacag aggaatttgt
     3841 attcgtcgcc atcaacggca taaagatcga tctgcgctgg cagattggtg ccggtattca
     3901 cttctttata catatccagc ggcgcgtttt gcgagtagcg cagattatct tcgatataag
     3961 tgttatagac accgcgcgcc agcgccgctt catcaccacc accggtccat acacgctgcc
     4021 cttttttacc aacaataatc gcggtgccgg tatcctgaca ggttggcaga acgcctttcg
     4081 ccgcgatgtc ggagttacgc aggaattgca gcgccacata tttatcattt tcgctggcct
     4141 ccgggtcacg cagaatgtcg gccacctgtt gttggtgagc cggacgcagc ataaacgacg
     4201 catcatgaaa cgcctgacgc gccaacagag ttaacgcttc gggtgcgact ttcaaaatct
     4261 cctgcccttc aaattcagat acgctaacgt gttcactggt tagcaggtaa tactcagtat
     4321 catctttttt gagtggaaaa ggagactgat aatgaaaggg tttgtttgac attgttctct
     4381 cacttactgc ctggtttggt tatgctctgg gcgggtgttc cgttgccctg ttaaaagcga
     4441 gtaacaatat cctacacagt tttttaacaa aaactgagac tagtacgact ttttgcggct
     4501 ccaggttact tcccgtagga ttcttgcttt aatagcggga ttaatttcca cattaaaaca
     4561 gggattgatc atgcaaaaac tcattaactc agtgcaaaac tatgcctggg gtagcaaaac
     4621 ggcgttgact gaactttatg gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg
     4681 gatgggcgca catccgaaaa gcagttcacg agtgcagaat gccgccggag atatcgtttc
     4741 actgcgtgat gtgattgaga gtgataaatc gactctgctc ggagaggccg ttgccaaacg
     4801 ctttggcgaa ctgcctttcc tgttcaaagt attatgcgca gcacagccac tctccattca
     4861 ggttcatcca aacaaacaca attctgaaat cggttttgcc aaagaaaatg ccgcaggtat
     4921 cccgatggat gccgccgagc gtaactataa agatcctaac cacaagccgg agctggtttt
     4981 tgcgctgacg cctttccttg cgatgaacgc gtttcgtgaa ttttccgaga ttgtctccct
     5041 actccagccg gtcgccggtg cacatccggc gattgctcac tttttacaac agccacatgc
     5101 cgaacgttta agcgaactgt tcgccaacct gttgaatatg cagggtgaag aaaaatcccg
     5161 cgcgctggcg attttaaaat cggcgctcga cagtcagcag ggtgaaccgt ggcaaacgat
     5221 tcgtttaatt tctgaatttt acccggaaga cagcggcctg ttctccccgc tgttactgaa
     5281 tgtggtgaaa ttgaatcctg gcgaagcgat gttcctgttc gctgaaacac cgcacgctta
     5341 cctgcaaggc gtggcgctgg aagtgatggc gaactccgat aacgtgctgc gtgcaggtct
     5401 gacgcctaaa tacattgata ttccggaact ggttgccaat gtgaaattcg aagccaaacc
     5461 ggctaaccag ttgttgaccc agccggtgaa acaaggtgca gaactggact tcccgattcc
     5521 tgtggatgat tttgccttct cgctgcatga ccttagtgat aaagaaacca ccatcagcca
     5581 gcagagtgcc gccattttgt tctgcgtcga aggcgatgca acgttgtgga aaggttctca
     5641 gcagttacag cttaaaccgg gtgaatcagc gtttattgcc gccaacgaat caccggtgac
     5701 tgtcaaaggc cacggccgtt tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa
     5761 aattaacatc tcttgctaag cttattaaag gcttataaca ccttcaggcg gccagtccgc
     5821 ctgatttcat tttatggata atcattatga ataaatcgct ggtagcggta ggcgtcattg
     5881 ttgcgctagg cgtagtctgg acaggcggcg catggtatac aggcaagaag attgaaaccc
     5941 atctcgaaga catggtcgcg caggcgaacg cgcaactcaa actgacagct cctgaatcca
     6001 acctggaagt gagttatcaa aactatcatc gcggcgtatt cagcagccag ttgcaactgt
     6061 tggtgaaacc cattgccggg aaagaaaatc cgtggattaa aagcggtcag agcgtcatct
     6121 tcaacgaatc ggttgatcat ggtcccttcc cgcttgccca gcttaaaaaa ctgaacctga
     6181 tcccgtcgat ggcatcaatt caaaccacgc tggttaataa cgaagtaagc aaaccactgt
     6241 ttgatatggc aaaaggtgaa acgccttttg agattaactc gcgcattggt tacagcggtg
     6301 attccagttc cgatatttcg ctcaatccac tgaattacga gcaaaaggat gaaaaagtcg
     6361 cctttagcgg cggcgagttc cagttaaatg ctgacagaga cggcaaagcc atctcccttt
     6421 ccggggaggc gcaaagtggt cggatagacg cagttaacga atacaaccag aaagtgcagt
     6481 tgacctttaa taatctgaaa accgacggtt ccagcacgct ggcaagtttt ggtgagcgcg
     6541 taggaaacca aaaactgtca ctggaaaaaa tgaccatttc agtggaaggc aaagaactgg
     6601 cactgctgga aggcatggag atcagcggta aatcggatct ggtcaatgac ggtaaaacga
     6661 tcaatagcca actggattac tcgctaaaca gcctgaaggt acagaatcag gatctgggca
     6721 gcggcaagct gactttaaaa gtcggccaga ttgatggtga agcctggcat cagtttagcc
     6781 agcaatataa cgcgcaaact caggcgctgc tggcacagcc agaaattgcc aacaatcccg
     6841 aactttatca ggagaaagtg acggaagcct tctttagcgc cctgccgctg atgttgaaag
     6901 gcgatccggt gattactatc gcgccgctaa gctggaaaaa cagtcagggt gaaagtgcgc
     6961 tgaatctgtc gctgttcctg aaagatccgg caacgactaa agaagcgccg caaacgctgg
     7021 cgcaggaagt agatcgttcg gttaaatctc tggatgcgaa actgaccatt ccggtggata
     7081 tggcaactga gttgatgact caggtagcga agctggaagg ttatcaggaa gatcaagcga
     7141 aaaaactggc gaaacagcaa gttgaaggtg catcagcaat ggggcagatg ttccgtctga
     7201 ccaccttgca ggacaatacc atcaccacca gcctgcaata tgctaacggt cagataacgt
     7261 taaatgggca gaaaatgtcg ttagaagatt ttgttggcat gtttgcgatg ccagctctta
     7321 acgttccggc tgtaccggca attccgcagc agtaattcac ggccaaaacg ccggagaatt
     7381 ttcactccgg cgttttcatc tgaaaacaaa ccagagattg cctgatgcgc tacgcttatc
     7441 aggcctacct tttcctgcaa cactttgaat ttatgagttt ttgtaggctg gataaagcgt
     7501 ttacgccgca tccggcataa aaaacgcgca ctttgtcaac aatctgaaac gccggaggtt
     7561 tttctctccg gcgttatttc ttacttcagc ataaagtcat actgaatact cattttcaca
     7621 tcccagtaat tcaggcgatc gcccagcgta ctgccatcgc gcttttcccg ccctttatcg
     7681 gcagcaagaa agaaattcat cccttgtaac ggcccttcgc tgacccgata gttcatacca
     7741 cctaagatac ccgcgccttc aatccgcaca tctcgtttac tggattcata gccgcgagtc
     7801 acaatcggtg caaatgtcag cgtaaattgc ggagttacac ggtagttgac acctaattgc
     7861 caggactgca ttccttcacg gttacgatca agacttaatg aaaagggata gccaatgtcg
     7921 gtatccacca cggcaccatt cattgcatgg cgcaaataac caccactgcc aaatacgctt
     7981 ccccacggcg cattctaggt caattgtcca ctaaccaacg ccgtttcatt gggctggctg
     8041 gtattacgac ttaaaccttc gagctgcgcg taaaagccga gcaactcccc tttcaacgta
     8101 taattcgggt cgagcggtat gcttttctcg agaatacttt gtatctgatt ggaaacatct
     8161 ttctgtaccg atgcccccag agaatagcga ctggtatcat catgccaact ggcggctaaa
     8221 aaagaacgtg cgcgatgttt atcgtatttt cccgtctggc tgttatacac cccttcatca
     8281 cgaataccgg ttgttgaatc agtccataaa aatactcgtg aacgccacat tccccacagt
     8341 tcaatatttg gcgttaaagc ccaataacct aatgctcctt cagaaactgt cggtaatgta
     8401 cgttgcgaac tgccagccat taacggaata cgataaggta agctaccata actataatcc
     8461 gtcccgaagc gacctaattt aagcaggcta ttttcactgg gttttatttt tactgccccc
     8521 agggcaaagc caaaactttt atcaccatca agataccacc gggtactcat atcagcacga
     8581 gcacctaatt tataaacata atacgctccg ccttcaacgc caataatatt ggaataataa
     8641 ccactattga aatcgagcaa tcctccctgt acccatgcgt aaatatctct tcccgcgcca
     8701 gcactgggct tatcagccct gcgcaattcg tttttcagtc tgatgcgtag tcccgcttca
     8761 tcgtcagcca gttgcgccgc gtaagcagag gtaaggccag aggcagccgt caggcaaata
     8821 acggccatgg ccactatttt tctcatagtt gcacctttga tgttctgatt atttttattg
     8881 aatattaatt agtgatatcg ctgattaatt gctgctgcac ttttttacga ttatcaattt
     8941 caaccacgat ttctttgaat tttttatccg tgagcggata aaaccagata ataacgaatg
     9001 ccagtagcat aaatccgcaa ggtactaagg caattgatgt gcggatgccc ataataactt
     9061 ccggcgttta cacctgattg gcgatatatc cgcttaatcc caaaataaag gcaggaattg
     9121 aacctccgat tgcctgaccg catttacggg taaatgagaa tagtgaatag gtgagccctt
     9181 caattcgcac gccggtcagg tattcaccgt attctacggt atcagcttcc agtgcccaca
     9241 tcacagtcat agtaacgccc tgaccaagtg aacagcccgg tatcatttaa cacatagcgc
     9301 acgtagaaca acgacgaggc gctgaccgcg aaggtcgaaa tcagcacaca cagcgcaccg
     9361 atgcacaaca taaacagcgg gcgattccgt ttcagggttt gcaaactgat cttcaatgac
     9421 ggctgcgcca cgatacgtac cacattttca cgcgtcgatt tgaagcagat gaagtaaagc
     9481 accattccgg caatcgccag cacaatcgtc cagaaatggt ataccgacac catctcttcc
     9541 gggctggagt tcttaatgct cggtcctatc agaaatgcca ggcatacaaa ggtcaatgaa
     9601 gcggcaatcc aacgagccgc gcccagacgg gcgcgggatt gtggttgttg ggtcatcgcg
     9661 gtagcaagtg aaccataagg aatattcacc aggctgtagc aaagcccgag gcccatgtag
     9721 gtcaaatatg catacaccac tttgctacca tggctccagt cggtcggtac ccagaatacc
     9781 agcacgctga agatcattaa cggcgcagta ccgaagagta aaaacgggcg gaattttccc
     9841 cagcgggtat tcacactgtc caccactcgt ccggcaaaga cgtcggcgaa ggcatcgaat
     9901 acccgcacca gtaacagcat ggtgcctgcc gcagcggcac cgacgccagc gacgtcggtg
     9961 tagtaactca ataggaagag cgcccccatt gcgaaggcga agttattggc gacgtcaccg
    10021 aggctgtagc cgacgatggt gcgccaggag agttgttgat tcattgtttg cctccctgct
    10081 gcggtttttc accgaagttc atgccagtcc agcgtttttg cagcagaaaa gccgccgact
    10141 tcggtttgcg gtcgcgagtg aagatccctt tcttgttacc gccaacgcgc aatatgcctt
    10201 gcgaggtcgc aaaatcggcg aaattccata cctgttcacc gacgacggcg ctgacgcgat
    10261 caaagacgcg gtgatacata tccagccatg cacactgata ctcttcactc cacatgtcgg
    10321 tgtacattga gtgcaggccg gctaacgtat ccacgccgta ttcggtgatg ataatcggct
    10381 gatgcagttt ctcctgccag gccagcagtt ctttttccag taccttctct gccgtttcca
    10441 aatcgccgct ttggacatac caaccgtaat aacggttcag gcacagcaca tcaaagagat
    10501 cgctgatggt atcggtgtga gcgtcgcaga acattacatt gacgcaggtg atcggacgcg
    10561 tcgggtcgag tttacgcgtt gcttccgcca gtggcgcgaa atattcccgt gcaccttgcg
    10621 gacgggtatc cggttcgttg gcaatactcc acatcaccac gcttgggtgg tttttgtcac
    10681 gcgctatcag ctctttaatc gcctgtaagg gcgcctgctg agtttccccg ttgacagcct
    10741 cttcgctgta cagttctttc ggcttgttgc ccgcttcgaa accaatgcct aaagagaggt
    10801 taaagccgac agctgcagtt tcatcaatca ccacgatgcc atgttcatct gcccagtcga
    10861 gcatctcttc agcgtaaggg taatgcgagg tacggtagga gttggcccca atccagtcca
    10921 ttaatgcgtg atcgtgcacc atcagcacgt tatcgaatcc tttgcctcgc aaatccgcat
    10981 cttcatgacg accaaagcca gtaaagtaga acggtttgtg gttaatcagg aactgttctc
    11041 ccttcactgc cactgaccgg atgccgacgc gcagcgggta gatatcacac tctgtctggc
    11101 ttttggctgt gacgcacagt tcatagagat aaccttcacc cggttgccag aggtgcggat
    11161 tcaccacttg caaagtcccg ctggtgcctt gtccagttgc aaccacctgt tgatccgcat
    11221 cacgcagttc aacgctgata tcaccattgg ccaccacctg ccagtcaaca gacgcgtggt
    11281 tacagtcttg cgcgacatgc gtcaccacgg tgatatcgtc cacccaggtg ttcggcgtgg
    11341 tgtagagcac tacgctgcga tggatcccgg catagttaaa gaaatcatgg aagtaagact
    11401 gctttttctt gccgttttcg tcggtaatca ccattcccgg cgggatagtc tgccagttca
    11461 gttcgttgtt cacacaaacg gtgatacgta cacttttccc ggcaataaca tacggcgtga
    11521 catcggcttc aaatggcgta tagccgccct gatgctccat cacttcctga ttattgaccc
    11581 acactttgcc gtaatgagtg accgcatcga aacgcagcac gatacgctgg cctgcccaac
    11641 ctttcggtat aaagacttcg cgctgatacc agacgttgcc cacataatta cgaatatctg
    11701 catcggcgaa ctgatcgtta aaactgcctg gcacagcaat tgcccggctt tcttgtaacg
    11761 cgctttccca ccaacgctga tcaattccac agttttcgcg atccagactg aatgcccaca
    11821 ggccgtcgag ttttttgatt tcacgggttg gggtttctac aggacgtaac ataagggact
    11881 cctcattaag ataaaaatac tggtcaacct ttaatctgat tagttaacca attctgtgtt
    11941 gcacacagtt atctgtgaga aacccatgga agagaaatgg aaataaaaag aactgacacc
    12001 gcgatcaaaa aaccaaaaac tgcaataccc acagcgtgat atggatcgca ttaatcttta
    12061 aaacagcgtt gacagaagcc ataaaataag caatcgaacg ttcgttcgag agtattggga
    12121 acgataaact ctcatgacat atggctacgg cgaatatttt gggggggaat tctccgaaca
    12181 cggggaggat aatttttgca cagagaaatt acgatatgaa acgcgggcac agttatcacc
    12241 agcccgcgaa atgagaggat caggatgcgg ttaatatacc gccaatcatc gcgcgtaatc
    12301 cctgagcaaa cgacgcttca cgcgccaggt cttccgcttc gatatctgcc agtacaccgt
    12361 aggtcagatc cagcagtaaa cgtgaagtca ttgccgtgtt gatgtctggg cttatctcgc
    12421 ctttttgctg ggcatcacgc atccgctggg caacaaattc agtgatcgtc atatgcttat
    12481 ttttaagcat ggcggcgacc tgtgggttac gcatcccttc cgccataatt tcgaccacca
    12541 gcgcccgttg cccaaaggct tcatgggtga gagagacaat agactcgacc atatagtcaa
    12601 caaaatgaat cccttcaatc ggttcccgga aacgagccag cgccctctcc tggtcctgta
    12661 agataatcgc ctgaatcaag gcttctttgg agatgaaatg gtgatagagc gtcccaggac
    12721 taatggcgca agatttacag atggctttca tcgaggcact gtgaaatcca ttttctgaaa
    12781 aaatctctct ggcagcattg aggatccggg tccgtgtcgg ttgtgcttca gtctgcatgt
    12841 tatccatcat taaaccatta tagttagcgt gaataactag tttaccgcgt tgtcaggtga
    12901 gcgcctatca ttgctgttga atagtgcgaa ggtacactct attcgta
//
LOCUS       DBKEKI010000112        12849 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.112, whole genome shotgun sequence.
ACCESSION   DBKEKI010000112 DBKEKI010000000
VERSION     DBKEKI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.112"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..748)
                     /locus_tag="ACUQPX_003126"
     CDS             complement(<1..748)
                     /locus_tag="ACUQPX_003126"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HHV9057749.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASI"
     gene            complement(748..996)
                     /locus_tag="ACUQPX_003127"
     CDS             complement(748..996)
                     /locus_tag="ACUQPX_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HHV9057750.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(1028..1942)
                     /locus_tag="ACUQPX_003128"
     CDS             complement(1028..1942)
                     /locus_tag="ACUQPX_003128"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HHV9057751.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(1939..3626)
                     /locus_tag="ACUQPX_003129"
                     /pseudo
     CDS             complement(1939..3626)
                     /locus_tag="ACUQPX_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            4030..5172
                     /gene="yghO"
                     /locus_tag="ACUQPX_003130"
     CDS             4030..5172
                     /gene="yghO"
                     /locus_tag="ACUQPX_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HHV9057752.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQDFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(5296..6066)
                     /gene="glcC"
                     /locus_tag="ACUQPX_003131"
     CDS             complement(5296..6066)
                     /gene="glcC"
                     /locus_tag="ACUQPX_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HHV9057753.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            6317..7816
                     /gene="glcD"
                     /locus_tag="ACUQPX_003132"
     CDS             6317..7816
                     /gene="glcD"
                     /locus_tag="ACUQPX_003132"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HHV9057754.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVDVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            7816..8868
                     /gene="glcE"
                     /locus_tag="ACUQPX_003133"
     CDS             7816..8868
                     /gene="glcE"
                     /locus_tag="ACUQPX_003133"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HHV9057755.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            8879..10102
                     /gene="glcF"
                     /locus_tag="ACUQPX_003134"
     CDS             8879..10102
                     /gene="glcF"
                     /locus_tag="ACUQPX_003134"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HHV9057756.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            10107..10511
                     /locus_tag="ACUQPX_003135"
     CDS             10107..10511
                     /locus_tag="ACUQPX_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HHV9057757.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            10533..12704
                     /gene="glcB"
                     /locus_tag="ACUQPX_003136"
     CDS             10533..12704
                     /gene="glcB"
                     /locus_tag="ACUQPX_003136"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HHV9057758.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
BASE COUNT         3155 a         3267 c         3513 g         2914 t
ORIGIN      
        1 caatcgaggc cagactttta ctgaaagtac caacgataat atccacatca tcttcaacgc
       61 cgacagcttc agccagtccg cgccccgttg cgcctaacac gccaaacgaa tgcgcttcat
      121 cgacaatcaa ataaccaccg agacgacgtt tgatatcaac aatttccgcc agcggcgcaa
      181 cgtcaccgag catgctgtag atgccctcaa cgatgataat cgcctctttc gcccgttcac
      241 cgaggcgaac catgcgccgt tccagatctt tagcatcgtt atggcggaaa cgaataattt
      301 ccgcgccacc gagagaacag gcgtcataaa tactggcgtg gctgtcggca tccagtaaca
      361 ccacggcgtt atggtcggca agtgcgctga tgacgcctaa attagcggta taaccggttg
      421 aaaaaacgat agcggtaggg cgattgaaaa actcggcaat ctctttctct aacgccaggt
      481 gcggcgcata actgccgttt gccatacgag aaccggtggt gccggtgcct tgggccgcta
      541 acgcggcctg accttcgctg atggcatcat gattaaaggt taaaccgaga taattattgg
      601 tcccggccag aatcactttt ttattgccga tccggccttc ggtggcggaa tacacttcat
      661 caatacaggt gccaaacggt gttaacccgt tgtcactaaa ttgcagacgc tcgccagcaa
      721 ggcgcgcata tttatcgtat agccccatta tttattctcc agccatggaa gtagagtttc
      781 cattaattgc gcaggggttt tgacatccag cagaatatta ataggaatgg aaatatcgaa
      841 tctgtcttcc agcatcatta agagatccat cactttgatc gactccaggc caagatcgtt
      901 gacgagatcg ctgtcgggct taatttccac accgttttcg accaaatcct gtaaacagga
      961 aaggatgtaa tccattacta tttcacgatt taccatagaa acgttaacac acctttaaac
     1021 aagacaatca aaatagcccg ttgcgtaatg cgtgttccag gctaatcccg ggaaaccaat
     1081 taatatcttc agaaatacgg ttattacttg ccgaccagtc ggcgtgggtt aattcacgaa
     1141 ttttcgagcg ggtcagcata ggttctttac ctgccaggcg actcaacgcg gtactgatat
     1201 ccgcaaggcc tgtgagtacc ggcagaggaa tgtcaaccat tcgcacggaa ccacaacggg
     1261 ctttggcggc aagctgtcgt acgcgttgcc agtcatagcc accggcgacg ccatcgcata
     1321 attcataggt ttgcgtctgt acagtttcgg cgcttaacca ctgacccact gcttgcgcaa
     1381 aatcggtgac gtgaagaaaa gagagttgtg tatctggcgc accaagtcgt ggcagcaggc
     1441 cgcgtagcat ccagtcaaac agcggtttta actctttatc gccgggacca tacacggctg
     1501 tcgggcgaaa aacgcccagc gtaatttcgt ccgccattgc tgccagtcgt tgctcggcga
     1561 cgtatttgga ttttgcatac caggagagct cgggatggcg cgccgccagc gaagagataa
     1621 acagaaaacg ttggcaaaag ccgctctctt ttgctgcttg catcaggcgc aggctgccgt
     1681 caacgttaca gcgggtgaaa atctcttctt tgtgcccgcg cacttgcccg gcgcaatgga
     1741 ccactacgct ggctccggca accagctcgc taagtgaatg agtatcttcc agcgaaccgc
     1801 gcacccaggt gagattatca ttgacgtgag cgcgggcagt acgcgtcaat gcgcgaacgt
     1861 gaaagccgcg ggcgagcagg ttatcgataa tatatttacc gataaacccg gtagcgcccg
     1921 tcaccgcgac agtttgattc atgccaggga ttcctgcaca ttaagactgg cagcataagc
     1981 cttctgataa cgttttttcg cttccgcacg ggcaggcttg ccggaggacg ttcggggaat
     2041 actgtggggc ggcaacagat cgatagccgc ggtcacgcca aattcgcttt ggatccgtgc
     2101 cgccagcgcg tggataagct gcccgcgacg ttcttcgtcg ctgatccgac actggatctg
     2161 caaaatgatt ttttcctggg cggtaacaaa agcaatcgca tcgccagaat gaatttccgg
     2221 ttcctgttcc gctatatatt caatatcctg cggccagata ttacggccac gaataataat
     2281 cagatcttta atgcgtccgg tgacatacag ataaccgtcc agcagataac cgaggtcgcc
     2341 ggtgtctaac cagcccgtcg cggcaatctc gtcttgcgaa acctggtcgc caaagtaacc
     2401 gctcatcaga ctgggaccgg agatgcaaat atggcctacc acacgttccg cgaccggcat
     2461 acctgcttca ttgcggattt caataccatg ttccggcaac gctttgccgc agttgacgaa
     2521 agtcgatacg gcgcgtgtct ctgcacccgg cgcgacggct ttaccctgat attcgaggat
     2581 gtcgcgatcc acttcgttaa ccacaacccc ggaggcttca tcagagaagc tgacagccag
     2641 cgcattttct gccagtccgt agcacggcat gaaagtttta ttgtcaaagt taacctgacg
     2701 gaaacattca gcgaattgat ggagttgttc tgcggagatc ggctcagcac caataccagc
     2761 gacgcgccag caggaaagat ccagttcagc gagatctttt tcattcacgc ggcgttggca
     2821 caattcatag ccaaacggcg gcgcaacgga aacggtgccg cgatttttac tgatcaattt
     2881 aagccattgc aaaggacgca tggcaaaatc ctgagtgcac aaataatcta ctgaaagttg
     2941 cgtggcgacg gggtcaggag aaagccgacc agtcccatat catggtagaa aggctgccag
     3001 gagacgcagc ggtcgctagg gcgtaattta atgccatcgt ggcttatagc acgtagatta
     3061 gccatcactt cgcgatgggt gataatgacg ccacggggaa aacgggtgct gccggaggtg
     3121 tactggaggt aggcgatatc gttcggaact ggacgctgga gcgcaacatc ggcttccggt
     3181 aatgccttaa accaagcgtg gcttaaaaca tgtaattcgg ggttgttatg cgtcgcggca
     3241 ttgaccagtg gcaaccactc atcaccagtg ataatggctg cgggctggca acttgctagt
     3301 aaaccctgga gtttggcgct ccaggaatcc cgctgaccga cgcccattgg aatcgccaac
     3361 gggacggcga ctaagccagc atactgacag gcaaaaaaag cctctacaaa cccgctactc
     3421 gtttcggcaa tcagcgccac gcgttcgcct tttttcaggt tcagcgataa caaccgcttc
     3481 gcaccagctt cggcacgcgt ttttaatgtc tgatattcca gttgatcttc aagttggcaa
     3541 cgtctgtcat aaaaattcat tccggcgcta ctcagagcgg cgtagtccaa agcatcaacc
     3601 agcgttggaa aatcggcata gcgcataggt agggaatgcg taaagatttt attagacata
     3661 tacaccacga aaaaattgga tatcaaaaat aaaatgacaa taatgcggtg gattatatag
     3721 gcataaaaga taaatgagat agtattttca tcggtgtgaa aatgaacatt gatttaaata
     3781 atgagtttaa gttttttgtt tttattatga atgatattta atttatgaat atattaagaa
     3841 gaagccttgt ccggtcgcct taaatcgaca aaaggcattg aggcgtttct gtgtagcctc
     3901 tgaccttatc ataatcatta attaatacta tttattcgtc gtttgtattc attgagttga
     3961 ttattaagat gccgctcgtt gctgttgcaa cctgtttttc accattaatt atttgttatc
     4021 cacagagata tggaatgcga tctgctgatg attaaaattg aaaaagtaat taataaaaat
     4081 gaccttaagg catttatcgc ttttccgtca tcactttatc ccgacgatcc aaactggata
     4141 ccccctttat tcattgaacg caacgagcat ttgtctgcga aaaatccggg gacggaccat
     4201 atcatctggc aggcgtgggt ggcaaaaaaa gcggggcaga tagttgggcg tattaccgcg
     4261 caaatagata ccttgcaccg tgagcgttac ggtgaagata ccggtcattt cggcatgatt
     4321 gacgccattg atgatccgca ggattttgcc gcgttgtttg gcgcagcgga agcgtggttg
     4381 aagtcacaag gtgcaagtaa gatcagcggg cctttcagcc tgaatatcaa tcaggaaagc
     4441 ggattactga ttgaaggttt tgacacacca ccctgtgcga tgatgccaca cggcaaaccg
     4501 tggtatgccg cgcatattga acaactgggt tatcacaaag gcattgattt actggcgtgg
     4561 tggatgcagc gaaccgatct cactttctct ccggcgctaa aaaaactgat ggatcaggtg
     4621 cgcaaaaagg tgaccattcg ctgcatcaat cgtcagcggt ttgccgaaga gatgcagatc
     4681 ctgcgtgaga ttttcaactc tggctggcag cacaactggg gatttgtgcc gtttaccgaa
     4741 catgaattcg cgaccatggg cgaccaactt aaatatctgg tgccggacga tatgatctac
     4801 atcgctgaga ttgattctgc accctgcgcg tttattgtcg gcttgcctaa catcaacgag
     4861 gcgattgccg atctgaacgg atcgctcttt cccttcggct gggcaaaatt gctgtggcgt
     4921 ttgaaagtca gcggtgtgcg aactgcgcga gtaccgctga tgggcgtgcg cgacgagtat
     4981 cagttcagcc gcatcggccc ggtgattgcg ctgttattga ttgaagcctt acgcgatccg
     5041 tttgcccgcc ggaagatcga tgcgctggag atgtcatgga tcctcgaaac caatacaggc
     5101 atgaataaca tgctggaacg cattggggcg gagccgtaca agcgttaccg attgtatgaa
     5161 aagcaaattt aataccagat cctgtttagc gcaggggcgg tcattcggga gagggtatag
     5221 atgaacagat ttaacaccag atcctgttaa gtgcaggggc ggtcccctcg ccttggggat
     5281 agggtaaaac tctgctcaca ttcccacctt gttcatctcc agcggcaccg ccgagcgaat
     5341 caaatggtgg ccttccagct cgatatcgtg gagattcttt ttcacggtcc gtacatgatc
     5401 gcgcgctgcg cgctgagcga cgtgcggcag ccgctgcaac accgcgttgt agatccgcgc
     5461 atgctggcga tcgatctgct ttttttgctg tggtcgatgg tagagattat ttacgctggc
     5521 aaacactgaa ttaaacatca gatcggtcaa tgattgcagc gtaaacacca gcacctgatt
     5581 gtgagaagcc tgacaaatgg cgagatggaa agcgtgatcc aactgcgcat gttcgatcag
     5641 cgaaatctct ttgttgttct cactggcggc gagcattttt tcataacagc gggttatcac
     5701 aacaaaatca gcctgcgttc ccagcgttgc cgccagcctt gccgattcgc cctccagtaa
     5761 tgcgcgaacg tcgagcagat cgtacagcgt tcgcggctgc gtactgaaca gatggatcag
     5821 cgggctggtg tcctgcaccc gattaagccg tgcgacacga gaatcgcgac cctgcgccgt
     5881 ttcaataatc ccgcgcccgc gcagcacggt cagcccttca cgcagtgcgg agcgtgagaa
     5941 gccgagcttt tcacacagtc gacgttccga gggaagcggc tgaccgacct tcagtacgcc
     6001 gtcgataatt aaccgttcga tactctctgc aaccacttcg caaatagggc gacgttcatc
     6061 tttcattccc ggacctcgtg cacaggtagg accaattttt ctgttcttaa ctcgcaaaac
     6121 acgcacatca cgtaagtgat tatgttacat caatttaaca ttaagttaac caagacaagg
     6181 tcacagagct ggaaaaaaaa tggtctgacc ggtagctaaa gagatagacg aaaacgaaaa
     6241 gcccgcttaa taactgttca cagaagcagc gcgcaaaaat cagctgccac acaacacaac
     6301 aaagcgaagc ctactcatga gcatcttgta cgaagagcgt cttgatggcg ctttacccga
     6361 tgtcgaccgc acatcggtac tgatggcact gcgtgagcat gtccctggac ttgagatcct
     6421 gcataccgat gaggagatca ttccttacga gtgtgacggg ttgagcgcgt atcgcacgcg
     6481 tccattactg gttgttctgc ctaagcaaat ggaacaggtg acagcgattc tggctgtctg
     6541 ccatcgcctg cgtgtaccgg tggtgacccg tggtgcaggc accgggcttt ctggtggcgc
     6601 gctgccgctg gaaaaaggtg tgttgttggt gatggcacgc tttaaagaga tcctcgacat
     6661 taaccccgtt ggtcgccgcg cgcgcgtgca gccgggcgta cgtaacctgg cgatctccca
     6721 ggccgttgcg ccgcataatc tctattacgc gccggatcct tcctcacaaa tcgcctgttc
     6781 aattggcggc aatgtggcgg aaaatgccgg tggcgtccac tgcctgaaat atggtctgac
     6841 cgtacataac ctgctgaaaa ttgaagtgca aacgctggac ggcgaggcgc tgacgctggg
     6901 atcggacgcg ctggattcac ctggttttga cctgctggcg ctgttcaccg gatcggaagg
     6961 tatgctcggc gtgaccaccg aagtgacggt aaaactgctg ccgaagccgc ccgtggcgcg
     7021 ggttctgtta gccagctttg actcggtaga aaaagccgga cttgcggttg gtgacatcat
     7081 cgccaatggc attatccccg gcgggctgga gatgatggat aacctgtcga tccgcgcggc
     7141 ggaagatttt attcatgccg gttatcccgt cgacgccgaa gcgattttgt tatgcgaact
     7201 ggacggcgtg gagtctgacg tacaggaaga ctgcgagcgg gttaacgaca tcttgttgaa
     7261 agcgggcgcg actgacgtcc gtttggcaca ggacgaagca gagcgcgtac gtttctgggc
     7321 cggtcgcaaa aatgcgttcc cggcggtagg acgtatctcc ccggattact actgcatgga
     7381 tggcaccatc ccgcgtcgcg ccctgcctgg cgtactggaa ggcattgccc gtttatcgca
     7441 gcaatatgat ttacgtgttg ccaacgtctt tcatgccgga gatggcaaca tgcacccgtt
     7501 aatccttttc gatgccaacg aacctggtga atttgcccgc gcggaagagc tgggcgggaa
     7561 gatcctcgaa ctctgcgttg acgttggcgg cagcatcagt ggggaacatg gcatcgggcg
     7621 agaaaaaatc aatcaaatgt gcgcccagtt caacagcgat gaaatcacga ccttccatgc
     7681 ggtcaaggcg gcgtttgacc ccgatggttt gctgaaccct gggaaaaaca ttcccacgct
     7741 acaccgctgt gctgaatttg gtgccatgca tgtgcatcac ggtcatttac cttttcctga
     7801 actggagcgc ttctgatgct acgcgagtgt gattacagtc aggcgctgct ggagcaggtg
     7861 aatcaggcaa ttagcgataa aacaccgctg gtgattcagg gcagcaatag caaagccttt
     7921 ttaggtcgcc cagtcaccgg gcaaacgctg gatgtgcgtt gtcatcgcgg cattgttaat
     7981 tacgacccga ccgagctggt gataaccgct cgtgccggaa cgccgctggt ggcgattgaa
     8041 gcggcgctgg agagcgccgg gcaaatgctt ccctgcgagc cgccgcatta tggtgaagaa
     8101 gccacctggg gcgggatggt cgcctgcggg ctggcggggc cgcgtcgccc gtggagcggt
     8161 tcggtccgcg attttgtcct cggcacgcgc atcattaccg gcactggaaa acatctgcgt
     8221 tttggtggcg aagtgatgaa aaacgttgcc ggatacgatc tctcacggtt aatggccgga
     8281 agctacggtt gtcttggcgt gctcactgaa atctcaatga aagtgttacc gcgaccgcgc
     8341 gcctctctga gcctgcgtcg ggaaatcagc ctgcaagaag ccatgagtga aatcgccgag
     8401 tggcaactcc agccattacc cattagtggc ttatgttact tcgacaatgc gttgtggatc
     8461 cgccttgagg gcggcgaagg atcggtaaaa gcagcgcgtg aactgctggg tggcgaagag
     8521 gttgcgggtc agttctggca gcaattgcgt gaacaacaac tgccgttctt ctcgttacca
     8581 ggtaccttat ggcgcatttc attacccagc gatgcgccga tgatggattt acccggcgag
     8641 caactgatcg actggggcgg ggcgttacgc tggctgaaat cgacagccga tgacaatcaa
     8701 atccatcgca tcgcccgcaa cgctggcggt catgcgaccc gctttagtgc cggagatggt
     8761 ggctttgccc cgctatcggc tcctttattc cgctatcacc agcagcttaa acagcagctc
     8821 gacccttgcg gcgtgtttaa ccccggtcgc atgtacgcgg aactttgagg agcaggctat
     8881 gcaaacccga ttaaccgaag agatgcggca gaacgcgcgc gcgctggaag ccgacagcat
     8941 cctgcgcgcc tgtgttcact gcggattttg taccgcaacc tgcccaacct atcagcttct
     9001 gggcgatgaa ctggacgggc cgcgcgggcg catctatctg attaaacagg tgctggaagg
     9061 caacgaagtc acgcttaaaa cacaggagca tctcgatcgc tgcctcactt gccgcaattg
     9121 tgaaaccacc tgtccttctg gtgtgcgcta tcacaatttg ctggatatcg ggcgtgatat
     9181 tgtcgagcag aaagtgaaac gcccactgcc ggagcgaatg ctgcgcgaag gattgcgcca
     9241 ggtagtgccg cgtccggcgg tcttccgtgc gctgacgcag gtagggctgg tgctgcgacc
     9301 gtttttaccg gaacaggtca gagcaaaact gcctgctgaa acggtgaaag ctaaaccgcg
     9361 tccgccgctg cgccataagc gtcgggtttt aatgctggaa ggctgcgccc agcctacgct
     9421 ttcgcccaac accaacgcgg caactgcgcg agtgctggat cgtctgggga tcagcgtcat
     9481 gtcagctaac gaagcaggct gttgtggcgc ggtggactat catcttaatg cgcaggagaa
     9541 agggttggca cgggcgcgca ataatattga tgcctggtgg cccgcgattg aagcaggtgc
     9601 cgaggcaatt ttgcaaaccg ccagcggttg cggcgcgttc gtcaaagagt atgggcagat
     9661 gctgaaaaac gatgcgttat atgccgataa agcgcgtcag gtcagtgaac tggcggtcga
     9721 tttagtcgaa cttctgcgcg aggaaccgct ggaaaaactg gcaattcgcg gcgataaaaa
     9781 actggccttc cactgcccgt gtaccctaca acatgcgcaa aagctgaacg gcgaagtgga
     9841 aaaagtgtta cttcgtcttg gatttacctt aacggacgtt cccgacagcc atctgtgctg
     9901 cggttcagcg ggaacatatg cgttaacgca tcccgatctg gcacgccagc tgcgggataa
     9961 caaaatgaat gcgctggaaa gcggcaaacc ggaaatgatc gtcaccgcca acattggttg
    10021 ccagacgcat ctggcgagcg ccggtcgtac ctctgtgcgt cactggattg aaattgtaga
    10081 acaagccctt gaaaaggaat aaaaaaatga aaactaaagt cattcttagc cagcaaatgg
    10141 cgagtgcaat tattgccgca ggtcaggaag aggcgcagaa aaataactgg tctgtttcca
    10201 ttgctgttgc cgatgacggc ggtcatctgc tggcgttaag tcgcatggac gattgcgcgc
    10261 cgattgcggc ttacatctcc caggagaaag cgcgtaccgc cgcgctgggg cgtcgtgaaa
    10321 ctaagggcta tgaagagatg gtgaacaacg gacgtaccgc gttcgtgact gcgccgttac
    10381 tgacgtcgct ggaaggcggc gtaccggttg ttgttgatgg gcaaattatt ggtgccgtgg
    10441 gcgtttctgg tttaaccgga gcacaggatg cgcaggtcgc gaaagcggca gcagcggtgt
    10501 tggcgaaata agcgaaaacg aggagagaaa caatgagtca aaccataacc cagggccgtt
    10561 tacgcattga cgccaatttt aaacgttttg tggatggaga agttttgccg ggcgtggaac
    10621 tggacgctgc tgcattctgg cacaatgttg atgagatcgt tcacgatctg gcaccagaaa
    10681 atcgtcagtt gctggcagaa cgcgatcgca ttcaggcggc gcttgatgag tggcatcgca
    10741 gcaatccggg gccggtaaaa gataaagcgg cctataaatc tttccttcgt gaactgggct
    10801 acctggtgcc gcaaccggag cgcgtgacgg tggaaaccac gggcattgac agcgaaatca
    10861 ccagtcaggc ggggccacag ctggtggttc cggcaatgaa cgcccgctac gcgctgaacg
    10921 cggcgaacgc tcgctggggc tcactgtacg atgcgttata cggcagcgac atcatcccgc
    10981 aggaaggggc gatggtcagt ggctacgatc cgcagcgcgg tgagcaggtt atcgcctggg
    11041 ttcgacgttt cctcgatgaa tcactaccgc tggaaaacgg cagctatcag gatgtggtgg
    11101 cgtttaaggt ggtcgataaa caattacgca tccagttgaa aaatggtaaa gaaaccacgt
    11161 tacgtactcc agcacagttt gtcggttacc gtggcgatgc cgctgcgccg acctgcattt
    11221 tgctgaaaaa taacggcctg catattgaac tgcaaattga tgccaacggg cggattggca
    11281 aagacgatcc ggcgcacatc aacgatgtta tcgtcgaagc tgctatcagt accattctcg
    11341 actgcgaaga ttcggtcgcg gcggttgatg cggaagataa aatcctgctg taccgcaatc
    11401 tgctgggcct gatgcagggg actctgcaag agaaaatgga gaaaaacggt cggcaaatcg
    11461 tacgtaaact gaatgacgat cgtcagtaca ccgccgccga tggctctgaa atttctctgc
    11521 acggacgctc gttgctgttt atccgcaacg tgggtcattt gatgaccatt cctgtgattt
    11581 gggacagcga aggcaatgaa atcccggaag gtattcttga tggcgtcatg actggcgcga
    11641 ttgccctcta tgatttaaaa gtgcagaaaa actcgcgcac tggcagcgtc tatattgtga
    11701 aaccgaaaat gcacggtccg caggaagtgg cgttcgccaa caaactgttt acccgcattg
    11761 agacaatgct cggtatggca ccgaataccc tgaaaatggg cattatggat gaagaacgcc
    11821 ggacctcgct gaacttgcgt agctgtatcg ctcaggcgcg caaccgcgtg gcgttcatca
    11881 ataccggttt cctcgaccgt accggcgatg aaatgcattc ggtgatggaa gctggcccga
    11941 tgctgcgtaa aaaccagatg aaatcgacgc cgtggatcaa agcctacgaa cgtaataacg
    12001 tgctttccgg tctgttctgt gggctgcgcg gtaaagcgca aatcggtaaa ggcatgtggg
    12061 caatgccgga cctgatggca gacatgtaca gccagaaggg cgaccaactg cgtgccgggg
    12121 caaacacagc ctgggttccg tcaccaaccg ctgctacgct ccatgcgctg cactaccacc
    12181 aaaccaacgt acagagcgtg caagccaaca ttgcccagac cgagttcaat gctgaatttg
    12241 aaccgctgct ggacgatctg ctgactattc cggtggctga aaacgctaac tggtcggcgc
    12301 aagagatcca acaagagctg gataacaacg tgcaggggat tctggggtac gtggtgcgtt
    12361 gggtggagca ggggattggt tgttcaaaag tgccggatat tcacaacgtg gcgctgatgg
    12421 aagaccgcgc aacgctgcgt atctccagcc agcatatcgc caactggtta cgtcacggta
    12481 ttctgaccaa agaacaggtg caggcgtcgc tggagaatat ggcgaaagtg gttgatcagc
    12541 aaaacgctgg cgatccggct tatcgtccga tggcggggaa tttcgctaac tcgtgtgctt
    12601 ttaaagctgc cagcgattta atcttcctcg gcgtgaaaca gccaaacggt tataccgaac
    12661 cgttattaca cgcctggcgt ttacgcgaaa aagaaagtca ttaatagcgt caaaaaagcc
    12721 ccggcaagtt ttattgtcgg ggctatgata gtaactgcgg tagtacattt atcccggaaa
    12781 taaccggagc gattatcagg gtttaacaat attaaatacc cgtagcgcat tactagcggg
    12841 cctaaaaca
//
LOCUS       DBKEKI010000113        12804 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.113, whole genome shotgun sequence.
ACCESSION   DBKEKI010000113 DBKEKI010000000
VERSION     DBKEKI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.113"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..1269
                     /gene="citF"
                     /locus_tag="ACUQPX_003137"
                     /pseudo
     CDS             1..1269
                     /gene="citF"
                     /locus_tag="ACUQPX_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            1273..1824
                     /gene="citX"
                     /locus_tag="ACUQPX_003138"
     CDS             1273..1824
                     /gene="citX"
                     /locus_tag="ACUQPX_003138"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HHV9057759.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            1799..2673
                     /gene="citG"
                     /locus_tag="ACUQPX_003139"
                     /pseudo
     CDS             1799..2673
                     /gene="citG"
                     /locus_tag="ACUQPX_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
     gene            2724..4187
                     /gene="citT"
                     /locus_tag="ACUQPX_003140"
     CDS             2724..4187
                     /gene="citT"
                     /locus_tag="ACUQPX_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HHV9057760.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            4301..5107
                     /gene="rna"
                     /locus_tag="ACUQPX_003141"
     CDS             4301..5107
                     /gene="rna"
                     /locus_tag="ACUQPX_003141"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HHV9057761.1"
                     /translation="MKAFWRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            5336..5746
                     /gene="rnk"
                     /locus_tag="ACUQPX_003142"
     CDS             5336..5746
                     /gene="rnk"
                     /locus_tag="ACUQPX_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HHV9057762.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            5923..5973
                     /locus_tag="ACUQPX_003143"
     CDS             5923..5973
                     /locus_tag="ACUQPX_003143"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057763.1"
                     /translation="MAAIIVMAVLYLENHS"
     gene            complement(5977..7215)
                     /locus_tag="ACUQPX_003144"
     CDS             complement(5977..7215)
                     /locus_tag="ACUQPX_003144"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HHV9057764.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            7436..7864
                     /gene="uspG"
                     /locus_tag="ACUQPX_003145"
     CDS             7436..7864
                     /gene="uspG"
                     /locus_tag="ACUQPX_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HHV9057765.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(7985..9550)
                     /gene="ahpF"
                     /locus_tag="ACUQPX_003146"
     CDS             complement(7985..9550)
                     /gene="ahpF"
                     /locus_tag="ACUQPX_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HHV9057766.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(9795..10358)
                     /gene="ahpC"
                     /locus_tag="ACUQPX_003147"
     CDS             complement(9795..10358)
                     /gene="ahpC"
                     /locus_tag="ACUQPX_003147"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HHV9057767.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            10730..11476
                     /gene="dsbG"
                     /locus_tag="ACUQPX_003148"
     CDS             10730..11476
                     /gene="dsbG"
                     /locus_tag="ACUQPX_003148"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HHV9057768.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            11686..12587
                     /gene="citR"
                     /locus_tag="ACUQPX_003149"
                     /pseudo
     CDS             11686..12587
                     /gene="citR"
                     /locus_tag="ACUQPX_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor CitR"
     gene            12734..12796
                     /locus_tag="ACUQPX_003150"
                     /pseudo
     CDS             12734..12796
                     /locus_tag="ACUQPX_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3133 a         3283 c         3115 g         3273 t
ORIGIN      
        1 gggctttaaa aacctgaccc tggcgtccag ctccctgagt gattgccatg cgccgctggt
       61 agaacacatt cgccagggcg tggttacccg catttatacc tctggcctac gcggtccact
      121 ggcggaagag atctcccgtg gtctgctggc ggaaccggtg cagatccact ctcacggcgg
      181 tcgtgtgcat ctggtacaga gcggcgaact gaatatcgac gtggctttcc tcggcgtccc
      241 gtcctgtgat gaattcggta atgccaacgg ctacaccggt aaagcctgct gcggctccct
      301 cggctatgca atggttgatg ccgacaacgc aaaacaggtc gtgatgctta ccgaagaact
      361 gctgccttat ccgcataatc cggcaagcat tgagcaagat caggttgatt tgatcgtcaa
      421 agttgatcgc gttggcgatg ctgcaaaaat cggcgctggt actacccgta tgaccactaa
      481 cccgcgcgaa ctgcttattg cccgtagcgc tgcggatgtg attgtcaact ctggctactt
      541 caaagaaggt ttctccatgc aaaccggcac cggcggcgct tcgctggcgg taacccgttt
      601 cctggaagac aaaatgcgta gccgcgatat tcgcgccgac ttcgccctcg gcggtattac
      661 cgcgacgatg gttgatctgc acgaaaaagg tctgatccgc aaactgctgg atgtgcagag
      721 ctttgacagc catgctgcgc aatcgctggc ccgtaacccc aatcacatcg aaatcagcgc
      781 caaccagtac gctaactggg gtcgaaaggc gcatcggttg atcgtctcga cgtggtggta
      841 ctgagcgcgc tggaaattga caggcagttc aacgttaacg tgctgaccgg ttctgacggc
      901 gtactgcgtg gcgcttccgg tggtcactgc gataccgcga ttgcctctgc gctttccatc
      961 atcgtcgcgc cactggtacg cggtcgtatt ccgactctgg tggataacgt gctgacctgc
     1021 atcaccccgg gctccagtgt cgatattctg gtcacagacc acggtatcgc agttaacccg
     1081 gcacgtccgg aactagcaga acgtctgcag gaagcgggca ttaaagtggt ttccattgag
     1141 tggctgcgcg aacgtgcgcg tctgctgacc ggtgaaccac agccgattga attcaccgac
     1201 cgcgtcgttg ccgttgtgcg ttaccgcgat ggctcggtga tcgatgttgt gcatcaggtg
     1261 aaggaataag ccatgcacct gcttcctgaa ctcgccagcc accatgcggt gtcaattccc
     1321 gagctgctcg tcagccggga tgaaaggcaa gcacggcaac acgcctggct caagcaccat
     1381 cctgttccac tggtctcctt taccgtggtt gcgcctgggc cgattaaaga cagcgaggtc
     1441 acacgccgaa tttttaatca tggcgtgaca gccttgcgtg ccttagccac aaagcagggc
     1501 tggcaaattc aggagcaggc tgcactggtt tccgccagcg ggccggaggg catgttgagc
     1561 attgccgccc cggctcgcga cctcaagctc gccaccattg agcttgaaca tagtcatcct
     1621 ctcgggcggt tatgggatat cgatgtcctg acgcccgaag gcgaaattct ctcccgccgc
     1681 gactattcac tgccgcctcg ccgctgcctg ttgtgcgagc aaagcgcagc cgtctgcgcg
     1741 cgtggaaaaa cccatcaact gaccgattta ctcaaccgca tggaggcact gctgaacgat
     1801 gtcgatgcct gcaacgtcaa ctaaaaccac aaagcttgcg acgtcattaa tcgatgagta
     1861 cgccctgctg ggctggcgcg ccatgctgac tgaagtcaat ctgtcaccga aaccaggcct
     1921 cgtggatcgc attaactgcg gtgcgcacaa agatatggcg ctggaagatt tccaccgcag
     1981 cgcgctggcg attcagggct ggttaccccg tttcattgaa tttggtgcct gtagtgcaga
     2041 aatggcaccg gaagcggtac tccacggatt acgcccaatt ggtatggctt gcgaaggtga
     2101 tatgttccgc gccactgcgg gcgtaaacac gcataaaggc agcatttttt ctttaggcta
     2161 tgtgcggcaa ttggccgttt gcttcagctc aaccagccgg ttacgccaac aatcgtttgt
     2221 tctacggcgg caagtttctg ccgtggcctg accgatcgcg aactgcgtac caataattca
     2281 cgactgacgg caggtcaacg gttgtaccag cagcttggcc ttaccggcgc acgcggtgaa
     2341 gccgaagcgg gttatccact ggtgatcaac cacgccttgc cgcattacct cactctgctg
     2401 gatcaggggt tagatcctga actggcattg ctcgatacct tgctcctact gatggctacc
     2461 aacggcgata ccaacgttgc atcgcgcggt ggcgaggggg gcctgcgctg gctacagcgc
     2521 gaggcgcaaa cattattgca aaaagggggc attcgaaccc ccgccgatct cgattatctc
     2581 cggcagttcg acagggagtg tatcgaacga aatctcagtc caggcggcag tgccgacctg
     2641 ctgatcctta cctggttttt agcacagatt taattattta agcacttgat aaatttggaa
     2701 atattaattt tcggagaacc cgtatgtctt tagcaaaaga taatatatgg aaactattgg
     2761 ccccactggt ggtgatgggt gtcatgtttc ttatccctgt ccccgacggt atgccgccgc
     2821 aggcatggca ttacttcgct gtgtttgtgg caatgattgt cggcatgatc ctcgagccaa
     2881 ttccggcaac agcgatcagt tttattgcgg ttactatttg cgttattggc agtaattacc
     2941 tgctctttga tgccaaagaa ttagctgacc cagcgtttaa tgcgcaaaaa caggcgctga
     3001 aatggggcct ggctggtttt tccagcacca cggtatggct ggtatttggc gcatttattt
     3061 ttgcattagg gtatgaagtt tccgggttag gtcgtcgcat tgcccttttc ctggtgaaat
     3121 tcatgggcaa acgcacgctg acgttgggtt atgcgattgt cattatcgac attctgctgg
     3181 caccgtttac accgtccaac accgcgcgta ccgggggtac ggtttttccg gtcattaaaa
     3241 acctgccgcc gttgtttaaa tcattcccga acgatccgtc cgcgcgtcgt attggcggct
     3301 atttgatgtg gatgatggtc attagtacca gtctgagttc gtccatgttt gtcaccggtg
     3361 cggcaccaaa cgtgctgggt ctggagttcg tcagcaaaat tgccggtatc cagattagct
     3421 ggttgcagtg gttcctctgc ttcctgccgg ttggggttat cttgcttatc attgcgccgt
     3481 ggctttccta cgtgctgtac aaaccggaaa tcacacacag tgaagaagtg gcaacctggg
     3541 cgggtgatga actaaaaacc atgggtgcgc tgacacgcag agagtggacg ctgattggcc
     3601 ttgtattgct cagcttaggt ttgtgggtat ttggcagtga agtcattaat gctactgcgg
     3661 ttggtctgct ggcagtttcg ctaatgctgg ctctgcacgt tgtgccgtgg aaagacatta
     3721 cccgctataa cagcgcatgg aacacgctgg tcaacctggc aactctggtt gtgatggcta
     3781 acggcctgac tcgttctggt tttattgact ggttcgccgg taccatgagt acgcacctgg
     3841 aaggattctc accaaacgca acggtgattg tactggttct ggtgttctac tttgcacact
     3901 acctgtttgc cagcctgtct gcgcacaccg caaccatgct gccggttatt ctggccgtcg
     3961 gtaaaggtat tccgggcgta ccaatggaac aactgtgtat cctgctggtg ctgtctatcg
     4021 gtatcatggg ctgtctgacg ccgtatgcaa ccggtcctgg ggtgattatt tacggctgtg
     4081 gctatgtgaa atcaaaagat tactggcgtc ttggcgcaat cttcggggtg atttacatct
     4141 ctatgttgct gttggttggc tggccgattc tcgccatgtg gaactaatac gttcgccaaa
     4201 cgctgaataa atcatcacgc ccgccaggta agcccacctg gcgggctttt ttatgattta
     4261 atagatagta ttcagtgtat gtatgagttc cacacccatt atgaaagcat tctggcgtaa
     4321 cgccgcgttg ctcgcggttt ctctgcttcc cttatcttct gccaacgccg tagcgttgca
     4381 ggcaaaacag tatggcgatt ttgatcgcta tgtcctggcc ctctcctggc aaaccggatt
     4441 ttgccagagt caacacgatc gaaatcgtaa cgaacgagat gaatgtcgcc tgcaaaccga
     4501 aacgaccaac aaagctgatt ttctgaccgt acatggtctg tggccaggat tgcctaaatc
     4561 ggttgctgcc cgtggtgttg atgaacgccg ctggatgcgc ttcggttgcg ctactcgccc
     4621 aatcccgaat ctaccagaag cacgcgccag ccgaatgtgt tcatcgccgg aaaccgggtt
     4681 atcactggaa acggccgcta aactaagtga agtcatgcca ggagctggcg gacgttcctg
     4741 cctggaacgc tacgaatatg ccaaacacgg tgcctgcttt ggttttgatc cggacgcata
     4801 cttcggtacg atggtacgcc tgaatcaaga aattaaagag agcgaagccg gaaaattcct
     4861 tgcggataat tacggtaaaa cagtgagccg ccgtgacttt gacgccgcct ttgccaaaag
     4921 ctggggaaaa gagaacgtga aagcagttaa gctaacgtgc cagggtaacc ctgcgtattt
     4981 gactgaaatt cagatctcga tcaaagctga cgccatcaac gccccgcttt ctgcaaactc
     5041 attcttgcca caacctcacc caggtaactg tggcaaaacc tttgtgattg ataaagcggg
     5101 ttattaatcc tctgatttac aaaacacgct aattcgtctt ccataaccat gccgattaag
     5161 aattatacat aatgcgctgc attgaggaat tccctgaatg tgacgcaaat cacttcaaag
     5221 agtgagcaac agtcagtatc atcatgtgag ttaaaccctc gccgcctgac ggtgagggtt
     5281 ttcttttggg tatgtttatc tgcgacactc gcagtaccga cgacatggag taaaaatgtc
     5341 cagaccaact atcatcatta acgacctgga tgccgaacgc atcgatattc tgctggagca
     5401 acccgcctat gctggtttgc caatcgccga cgcgttaaac gcagagttgg atcgcgccca
     5461 aatgtgttcg ccagaagaga tgccacacga cgtggtgaca atgaacagcc gggttaaatt
     5521 ccgcaatctt agcgatggcg aagtgcgtgt gcgcacgctg gtgtatccgg caaaaatgac
     5581 cgatagcaat actcagcttt ccgttatggc tccggtaggt gccgcactgc tggggctgcg
     5641 cgttggcgat tccattcact gggaacttcc gggcggcgtt gcaacccacc ttgaagtgct
     5701 ggaactcgaa taccagccag aagctgctgg cgactacctg ctttaatccc atctgcacaa
     5761 gcgataactc gctttgccca gaggatgcac tcgcatcctc tttacgcaaa atcagcaatt
     5821 tttcctggtt ctcactgttc acttttgctg caaaattatt ccgtttcgtt tgtgggaata
     5881 accatggcag aagcatttta catcctgata ggatttctga ttatggcggc gattattgtc
     5941 atggctgtcc tttacctcga aaaccactcc tgacgatcat attgttcccc ccggcatcgc
     6001 attcaccaga cctgaaaccg ccttctgcgc cgcctctgcg ctttgtgcac cgggtaccag
     6061 aatcacctta cggcactcct cttcacgttt ttcgaaaatc tcatatcccc gggcggcctc
     6121 ttcaaacggc atatagtggg taacaatttc ttctggtttc agtaatcctt tctcaattaa
     6181 cggtaataat tctcccagcc atgcgtgaac gtgggtctgt cccattttaa acgtcaaccc
     6241 tttatcaaag gcgtcgccaa acaggaaacc gtgaataaat ccagcgtaga cgcccggtac
     6301 gctaacaatg ccgccacgcc tgaccgccgc aatacactgg cgcaacgctt taccgctgct
     6361 gccctccagt ttcaggttag tcagcaccgt ttccgtggtg ctgcctttcg cttcaaaacc
     6421 gacggcgtct attactgcat ccacgccccg gtgacctgcc gtttgttcaa taattgactg
     6481 tgccggatcg ctatcttcat cgaaattaat cgggatcgcg ccgtagcggt cagcggcgaa
     6541 acgcaagcgg tagggatgat gatcaacaac aaaaatctgt tccgcaccga gcaaccgtgc
     6601 acaggcgatt gtcaacaatc ccacaggacc agcaccatag actgcaacgc ttgaaccttg
     6661 ttggatctgc acattttttg ctgcctgcca tgccgttggc agaatatcag aaaggaaaag
     6721 tgctttatca tctgaaagca aaggcggtac tttaaacggc cccacattcc ctttagggac
     6781 gcggacatat tccgcctgcc cgccaggaac gccaccatac aggtgactat aaccaaacaa
     6841 tgccgcggga gcaggtatct gttttttatt gagcgcagcg cctttacccg cattggtatt
     6901 ttcgcaggcg gcatattgct gtaatcgaca gaaaaaacag tcgccacaag caatgacgaa
     6961 cggaattacc acccggtcgc ctttttgcaa attttttacg tccttcccgg tttcaacgac
     7021 ttcccccata aattcatgac caaaaatatc gccatgttta acctggggta ttttgcctcg
     7081 ataaagatgg aggtcagagc cacagatcgc cgttaccgta atacgcagaa taatatcatc
     7141 tgcctgttca atgcccggat cgggaacatt ttctacctga acgtgatgtg ggccgtgata
     7201 agtcaatgct ttcatgcaac ctccagagac agttcaaaaa agagtcagtc tttaagggta
     7261 gctatcacag cgggaaaacg ggcggtaccg caggttttgg gagaattcct ggtgttcagt
     7321 gagaatatat taatcacaga aaccgttcgt aaggaaaaag aaccagacaa atatcaggga
     7381 taaatattat aatcatggcg aatgatttgt tcatgattaa cagggagaaa gggttatgta
     7441 taagacaatc attatgccag ttgatgtatt tgaaatggaa ttgagcgaca aagctgttcg
     7501 ccacgctgaa ttcctcgccc aggatgacgg agttattcat ctacttcacg tactacccgg
     7561 gtcagccagc ctgagcctgc accgttttgc cgctgatgtg cgtcgttttg aagagcatct
     7621 gcaacatgaa gcagaagaac gtctgcaaac gatggtcagc cacttcacca tcgatccttc
     7681 ccgcattaaa caacatgtcc gttttggtag cgtgcgggat gaagtcaatg agttggcaga
     7741 agaactgggg gctgatgttg tagttatcgg ttctcgcaac ccatcgattt cgacccatct
     7801 gttaggttct aacgcctcga gcgtgatccg ccacgccaat ctgcctgtac tggtcgttcg
     7861 ctaacagata aaagtattta tgccgccctg cctggaacat cccggcagtg ctttttaatg
     7921 ccataaaaaa gccgccaggt ttgaccccgg cggctaagca attgcaggtg aatcttactt
     7981 cttcttatgc agttttggtg cgaatcaggt agtcaaaagc actcagagag gctttggcac
     8041 cttcgccagt ggcgatgatg atctgcttgt acggaaccgt cgtacagtca cccgctgcga
     8101 acacgccttt gacgttggtt tcacatttcg catcaatgat aatctcgccc atgcggttac
     8161 gttcgactgc gccttccagc cagttggtgt tcggcagcag accaatctgg acaaaaatac
     8221 cggccagttc gatgttgtga atatcgccgc tgacacgatc tcgatattcc agaccaacga
     8281 ctttgctgcc gtcgcctttc acttccgtgg tttgcgcatt cagaataatg tcgacgtttt
     8341 tcaggctgcg cagtttgtcc tgcagaacct ggtcggcttt catttctggc gcaaattcca
     8401 gcagcgttac gtgctcaacg atacccgcca ggtcgattgc cgcttccacg ccggagttac
     8461 cgccgccgat aaccgctacg cgtttgcctt taaacagcgg gccgtcgcag tgcgggcagt
     8521 aggtcacgcc tttagtgcga tactgatctt cgcccggaac gttcatgttg cgccattttg
     8581 cacccgtcgc cacgataatg ctgcgtgctt tcagtaccgc gccagaagct gtttcaatct
     8641 ggtgtagacc accttcaacc gctgccggga tcagtttgct ggcgctctgg ctgtcgatca
     8701 catcaacgtc gtattcatca acgtgaactt tcagtgcgcc tgccaacttc tgcccttcag
     8761 tcttcggtac agaaatgtag ttttcgatat caacggtatc gaggatctga ccaccaaaac
     8821 gttcgcccat cagaccggta cggatgcctt tacgtgcgga gtaaattgct gccgctgcac
     8881 ccgccgggcc ggaaccaacg attaatacgt cataagcatc acgcttgttc agctcttctg
     8941 ccgcacgttt ttccgcgcca gtatcaattt tggcaacgat ttcagtcaac gtcatgcggc
     9001 cctgaccaaa ctctttcccg tttacgaaca ctgccggaac gcccatcacg ttgcgatcgg
     9061 tgatttcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg cttgatgcgc gggttcaata
     9121 cgctcatcag gttcagcgcc tgcaccacgt ccgggcagtt atggcaagag agcgagtaat
     9181 aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg ctccagcaga gactgcgctt
     9241 ctttcgacgg atgaccaccg gtccacagca acgccagtac cagcgaggtg aactcgtggc
     9301 ccagcgggga gcctgcaaaa cgtggtccct ggttggaacc tgggttggtg atcaggaaag
     9361 acggcttacg cactggcaag ctgttatctt ctttaaaggt gactttgtct gacagttctg
     9421 cgatttcagc caacagttcc ttgatttctg ccgatttagc gctgtcatcc agcgtggcaa
     9481 ttaactcaac aggcttggtc aatttctcaa ggtaagcctt gagttgagtt ttcatatttg
     9541 tgtcgagcat gtttatctcc taggctttaa acatcatcat gcaagcggcc tctgcggcta
     9601 cctggatgca gcttgcatca tggtgcggtg aagaaaatcg ggtgccgcag cacccgaaga
     9661 attaggtgaa tttccgaagt atttcgcgtc agagcaaggc gactagcctg tgaatccccg
     9721 ggagcttaca caagtaagtg accggggtga gcgggcgacg ccaacgccgc tatggcgtga
     9781 aagacgaagg aaatttagat tttaccaacc aggtccagag acggagccag agttgcttca
     9841 ccttctttcc atttagccgg gcaaacttca cctgggtgag aagctacgta ctgagctgct
     9901 ttgattttac gcagcagatc agacgcgtca cggccaatgc cttcagcggt aacttcgatt
     9961 gcctggatga taccctgcgg gtcaacaacg aaggtcgcac ggtcagccag accttcatct
    10021 tcacgcatgt tgtcgaagtt acgggtcagg gcgccagtcg ggtcgccgat catcgcatat
    10081 ttgattttag cgatggtttc agagctgctg tgccatgctt tgtgggtgaa gtgagtatcg
    10141 gtagatactg cgtatacgtc tacgcccagt ttctgcagtt cttcgtagtg gtcagcaacg
    10201 tcacccagtt cggtcgggca tacgaaagta aagtcagccg ggtagaagaa gaagacgctc
    10261 cagcggcctt cggtatcttt ttcggtgatt tcgatgaatt cgccgttttt gaatgcctgg
    10321 tttttaaaag gtttaatttt ggtgttaatc aaggacatct atacttcctc cgtgttttcg
    10381 atgagatgta aggtaaccaa tttttgccga ttcggctaat gcgtttccat tatcaaatcg
    10441 ataagtttta ccttacaacc ttcgtaagac aactttgtga actttgcacc gccagcggcg
    10501 ataaaaagta aaatggcccc ctttccaggt gaccgtgtaa tctgaactac cctttggtat
    10561 cttcagcgcc tgcctgcagg ctgaggtgtt gcccttacaa atgcaacaac gacatggatt
    10621 acaacaccct cataaacaaa gggcaatcac ctgatctaag ctcttaccta tgacagtgat
    10681 aggttatgcc ttttactcga cttttgcact gactgaaaag gacaaattaa tgttaaaaaa
    10741 gatactttta ctggctctgc ttcctgcaat cgccttcgca gaggaacttc cttccccagt
    10801 aaaagcgatt gaaaaacagg gcattacaat catcaaaacg ttcgatgccc ccggaggaat
    10861 gaaaggttat ctcggaaagt atcaggatat gggcgtcacc atctacctga ctcccgatgg
    10921 taagcacgct atctctggtt acatgtacaa cgagaaaggt gaaaacctga gtaacacact
    10981 tatcgaaaaa gaaatttacg caccagccgg acgcgaaatg tggcaacgga tggaacaatc
    11041 ccactggctc ctcgacggta aaaaagatgc gccggtcatt gtctacgtct tcgccgatcc
    11101 gttctgccca tattgtaaac agttctggca gcaggcgcgc ccgtgggtag attctggcaa
    11161 agtgcaatta agaacattgt tggttggggt tatcaagcca gaaagcccgg cgacagcagc
    11221 ggcaattctt gcctccaaag atcccgcaaa aacctggcaa caatatgaag cctctggttg
    11281 caagcttaag ctaaacgtgc ctgcaaacgt aagtacagag caaatgaaag tgttaagtga
    11341 caatgagaaa ctgatggacg atctgggggc aaatgtcacg ccggctatct attacatgag
    11401 taaggaaaat acgctgcaac aggccgtggg gttgcccgat cagaaaacgc ttaatatcat
    11461 tatgggaaat aaataaaagg taattcatgg atggttgaac tatatccctg cttttcaacc
    11521 atcctaaaaa agcatgaatt gctcgctcta ccctaagtgg ctgtgctatt ttagaaaaac
    11581 ttaatcctga gttaagcaaa aaaacataat caataaaaat acatacgcaa aaaacattga
    11641 ttaagtgaat ataccatgga agaaaaatat aaaccggagt agtgtatggc caatctctac
    11701 gacttgaaaa agttcgactt aaatcttctt gtcatatttg agtgtattta ccagcattta
    11761 agcattagca aagctgctga atcactatat atcaccccat cagccgtgag ccagtcgtta
    11821 caacgcttac gtgcgcaatt taatgaccct ttattcattc gctcaggaaa aggtatcgcg
    11881 ccgacgacaa cagggttgaa tctgcatcac catcttgaga aaacctcaaa ggccttgaac
    11941 aaacaattaa tatagtcaat aagtccgaac taaagaaaaa ctttattatc tacggccctc
    12001 agctcatttc ttgctctaat aatagtatgc tgatccgctg cctacggcaa gatgcctctg
    12061 tcgagattga gtgccatgat atcctcatgt cagcagagaa tgctgaagaa ctgttggttc
    12121 accgcaaagc cgatctggtg atcacccaaa tgccagtgat cagccgttcc gtcatttgca
    12181 tgccattgca tacaattcgc aatactctaa tttgtagtaa taagcatccg cgaattactg
    12241 acaatagcac ttatgaacaa attatggcag aagagttcac ccaattaata tccaaatcag
    12301 caggagttga tgattttcaa atggaaatag acgagaagtt tatgaatcgc aaaatttcat
    12361 ttcgtggttc ctctttgctg acaataatta atagcattgc agttacagat ttactgggaa
    12421 tagtccccta tgaactttat aactcccacc gtgactttct taatttaaaa gagataaaac
    12481 tagaacatcc gttaccttca ataaagcttt acatttccta taataaatct tctttaaata
    12541 atttggtgtt ctcccgattt attgaccgtc taaatgagag tttttgatat ataactacag
    12601 acagctacta taactttcat ctatttattc acagcgcgaa tgaaaaataa aacactctgc
    12661 tttacagtgt ttactttata attaacagtc ttatctcact gttcaccctg taacgttcag
    12721 agagcgtctt cagatgtcta tttataaaat tcctcttccg ctcaatattc tggaagccgc
    12781 acgagagcgt attacctaga cact
//
LOCUS       DBKEKI010000114        12625 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.114, whole genome shotgun sequence.
ACCESSION   DBKEKI010000114 DBKEKI010000000
VERSION     DBKEKI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.114"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..311)
                     /locus_tag="ACUQPX_003151"
     CDS             complement(<1..311)
                     /locus_tag="ACUQPX_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HHV9057769.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     G"
     gene            complement(424..2358)
                     /gene="yeaG"
                     /locus_tag="ACUQPX_003152"
     CDS             complement(424..2358)
                     /gene="yeaG"
                     /locus_tag="ACUQPX_003152"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HHV9057770.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYDIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            2794..3540
                     /gene="mipA"
                     /locus_tag="ACUQPX_003153"
     CDS             2794..3540
                     /gene="mipA"
                     /locus_tag="ACUQPX_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HHV9057771.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3630..4391
                     /locus_tag="ACUQPX_003154"
     CDS             3630..4391
                     /locus_tag="ACUQPX_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HHV9057772.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(4442..5326)
                     /locus_tag="ACUQPX_003155"
     CDS             complement(4442..5326)
                     /locus_tag="ACUQPX_003155"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HHV9057773.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(5410..6405)
                     /gene="gapA"
                     /locus_tag="ACUQPX_003156"
     CDS             complement(5410..6405)
                     /gene="gapA"
                     /locus_tag="ACUQPX_003156"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HHV9057774.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            6747..7160
                     /gene="msrB"
                     /locus_tag="ACUQPX_003157"
     CDS             6747..7160
                     /gene="msrB"
                     /locus_tag="ACUQPX_003157"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HHV9057775.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            7202..7474
                     /locus_tag="ACUQPX_003158"
     CDS             7202..7474
                     /locus_tag="ACUQPX_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HHV9057776.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            7844..8851
                     /locus_tag="ACUQPX_003159"
     CDS             7844..8851
                     /locus_tag="ACUQPX_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HHV9057777.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FMCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVVARCQQI
                     CGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRVGMGFKPLDFSINDITAWNKSIIG
                     HMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQWREGFDAMVDKTAIKVIMTYDFD
                     E"
     gene            8878..10257
                     /locus_tag="ACUQPX_003160"
     CDS             8878..10257
                     /locus_tag="ACUQPX_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9057778.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            10274..11317
                     /locus_tag="ACUQPX_003161"
     CDS             10274..11317
                     /locus_tag="ACUQPX_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HHV9057779.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            11338..12174
                     /locus_tag="ACUQPX_003162"
     CDS             11338..12174
                     /locus_tag="ACUQPX_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HHV9057780.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            12179..>12625
                     /locus_tag="ACUQPX_003163"
     CDS             12179..>12625
                     /locus_tag="ACUQPX_003163"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HHV9057781.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLD"
BASE COUNT         3193 a         2906 c         3201 g         3325 t
ORIGIN      
        1 tggccctgac cactgccgga acctccgccg ccaccctggg gacgttcaat tcggtcgttc
       61 tgaacgaaat ggtcattgcc cggatgcacg cggtggcgca gaccgccacg cccctgatga
      121 aacattggtt cgctaatatc ttccgtggga atggatacgg attcgccgct gtcgacgtca
      181 gtcaccgaac gcttattaat ggcctcggag atcgactgtt taatttgcgc tttataacgg
      241 cgtaaaaaac gctggcgatt caccatgctt ttgtttttgc cgttcagacg ccggtcaata
      301 aaccaggtca tatgcccccc gtactacatt tgccaactgt atgctacgcc tgtgttgccg
      361 cttctcaggc ccacggttcg ctgtcgttgt aggcccggta gcatgtgtac cgtgccaggg
      421 ttgttaagac gatttacgta cgcgcaaata ccattcgcac agtaaacgca cctgtttacg
      481 ggtgtagcct ttttccatca tacggtcgac aaagtcgtcg tgtttcttct gctcgtcggt
      541 tgacgttttg gcgttaaacg agataaccgg caacagctcc tcggtattgg agaacatttt
      601 cttctcgata accgtgcgca gtttttcata gctggtccag ttcggattgc gtccgctgtt
      661 attcgctctg gcgcgcagta cgaagttaac aatctcgttg cggaaatctt ttggattact
      721 gatccccgcc ggtttctcga ttttctccag ctcggcgtta agagactcgc ggtcaaacag
      781 ctgcccggta tccggatcgc gatactcctg atcctgaatc cagaaatccg cgtaggtaac
      841 ataacggtcg aaaatgtttt gcccatattc ggaataggat tcaaggtagg ccgtctggat
      901 ctctttgccg ataaactcgg catatttcgg gatcagataa cctttcagga actccagata
      961 gcgttctgcc tgctcttgcg ggaactgctc gcgctcgatc tgctgttcca ggacgtagaa
     1021 cagatggacc gggtttgccg ccacttctac atgatcgaag ttgaacacgc gggagaggat
     1081 cttaaacgca aaacgcgtcg acagaccgtt catcccttca tcgacaccgg cgtagtcacg
     1141 atattcctga tacgacttgg ctttcggatc ggtgtctttc agactttcgc catcataaac
     1201 ccgcatcttt gaataaatgc tggagttttc tggctctttc aggcgcgaaa gaatggaaaa
     1261 acgtgacagt gtttcgagcg tgccaggggc gcatggggcg tgagtcaatt cactgtgatt
     1321 aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc aagcaatacg gcaccttcac
     1381 gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta ttacggaaag tgacccattc
     1441 ggactcgttc gagtgggcga gaataatccc gttgaacggc agggcggaga taccttccgt
     1501 cccgttgtag ttaccttcct gagtggcggt taacaaggga tgcagcactt ttatcggtgc
     1561 tttaaacatc tcaacgaatt ccatgatccc ctgattggcg cggcacagcg caccggaata
     1621 gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt ttacgaatat cgactttccc
     1681 aaccagcgcg gagatgtcct ggttgttctc atcaccgggt tccgttttgg cgatagcaat
     1741 ttgttgcaga attgacggcc agaccttcac tacccggaac ttagtgatat cgccaccaaa
     1801 ttcatgcagg cgttttgccg cccatggcga catgatggtg ccgagataac ggcgaggaat
     1861 gtcatactct ttttccagaa tctgagcatc ttcctgcgga ttgaaaagac agaacggatg
     1921 atcgttgacc gggctacgct caccgttggc gctcaataca taaatcggta cgagctgcat
     1981 taatgatttc agtcgctcag caagcgatga tttaccccca cccacaggcc ccagcagata
     2041 caggatttgt ttcttctctt ccagcccctg agccgcgtgt ttcagataag agacaatctg
     2101 ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc ggataacgtg caatgacccg
     2161 gttagaaaag agtcgagaaa gtctgggttc ctgggctgta tcgaccatga caggctcacc
     2221 gatagccatc aatagccgct cagccgcgtt ggcataagca ctgcgatctt gccgacaagt
     2281 ggtaagaaac tcctgcagtg tgaactcttc gtccttggca gcttcatatc gctggcgata
     2341 gtgatcgaat atattcatgg tatgccgtcc tttcgttttt tagcacaggt taagagccgt
     2401 tcatatgaat agtagaggct cccggaagag gtaaactgaa cgacgtctct tgctaaaaca
     2461 cagcaactct catgccattt tatcgacgat gagaacaacg tgcccggtga tacaccttca
     2521 aaattaagcg tagatggcaa atggaaaact tgcctgcacg gctttcttaa tttcaatggc
     2581 atatcaacaa ctcatccata aattttgcat aattaatgta aagaccaggc tcgccagtaa
     2641 cgctaaattc atttggctgt aagcgcggtg tcatccgcgg caggaaaatt aaacagttac
     2701 tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta ttgacgggta aactttgtcg
     2761 cccgctaaac atttgtttaa ggaatgatta attgtgacca aactcaaact tctggcactt
     2821 ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg aaggtaaatt ttccctgggc
     2881 gcaggcgtag gtgtcgttga gcacccatat aaagattacg ataccgatgt ttacccagta
     2941 ccggtaatca actatgaagg cgataacttc tggttccgtg gcttaggtgg tggttactac
     3001 ctgtggaatg acgcaacgga taaactttca attaccgctt actggtcgcc gctttacttc
     3061 aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg atgaccgtaa gagcaccatg
     3121 atggctggtc tgtcttatgc tcactttacc cagtacggtt acctgcgtac caccctggct
     3181 ggcgataccc tggataacag caacggcatt gtctgggata tggcctggtt gtatcgttac
     3241 accaacggtg gcctgaccgt gactccgggt attggtgtgc agtggaacag cgaaaaccag
     3301 aacgaatact attatggcgt atcgcgcaaa gagtccgctc gcagcggtct gcgtggctat
     3361 aacccgaatg acagctggag cccttacctt gagctgagcg ccagctacaa cttcctcggc
     3421 gactggagtg tttacggtac cgcgcgctac acccgtctgt ctgatgaagt tactgacagc
     3481 ccgatggtgg ataaatcctg gactggcctg atttctaccg ggatcaccta caaattctga
     3541 taatgcatct ttccagggca ttttgtgcat aaaaacaggg cgtcacgccc tgttttgcat
     3601 tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa aatgattcaa tttagtggcg
     3661 atgtctcact gccagccata gggcagggaa catggtatat gggcgaagat gccagtcagc
     3721 gcaaaacaga agttgctgca ctacgcgcag gcattgaact cggtttaacc ctcattgata
     3781 ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt tggggaagca ttaaccggtc
     3841 tgcgagagaa ggtctttctc gtctctaaag tctatccgtg gaatgctggc gggcaaaaag
     3901 cgataaatgc atgcgaagcc agtttacgcc gtctcaatac tgattatctc gatctctact
     3961 tattacactg gtctggcagt ttcgcttttg aagagactgt tgctgcgatg gaaaaattga
     4021 tcgcccaggg aaaaatccgt cgctggggcg tttctaacct tggttatgcc gatatgcagg
     4081 aactctggca gctgccgggg ggaaatcagt gtgccactaa tcaggtgctt taccatctcg
     4141 gttcacgagg gattgagtac gatctactcc cctggtgcca gcaacagcag atgccggtga
     4201 tggcttacag tccattagcc caggccgggc ggttgcgcaa tggactgtta aaaaacgcgg
     4261 tagtcaacga aattgcacat gtccaacaaa atgcagctgc gcttgaggtc gaactttcgt
     4321 cagcggaatt agctatgctg gataaggcat atccggcacc aaaaggaaaa actgcgctgg
     4381 atatggtgtg atgttgatac gtgccggatg catcgcaccc ggcacacaac attaacgcaa
     4441 attaacgttt ggcaacgcga atggattgcg ccagatgtgc aggtttctct ttggtgactt
     4501 tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa ggtcttgtag ccatcgtccg
     4561 gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt ccagccgaca acgttcagat
     4621 gatgctggtg gcctacggcg ataatacgat tcagcgcttc atcattaatc acgctgcaat
     4681 cttgcggatt cagatacacg cgatcggtac ggtcagggaa ggtctgaata ccgtcggtca
     4741 gtacatcttc tttcgcgtca ttcactttat caatgaagcg atcgcccagc ccactgacgc
     4801 ttaccttagc gatatcaccc acgttaaagt aggtatgcag ggcagaggtg gtttcaaatt
     4861 cgccatgtga ttcaagatcg atttcacagg ttttacccac gcggaaatgc gctaacagcg
     4921 taaagtcgtg cggccagaat tttttcgtct cttcgctttg tgtcaattca aaagtcagcg
     4981 ctacgccatc agcatcttca tgatgtgatt tcagcgtcca cggcaggttg cgggcaaaac
     5041 cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca cggccagcaa accggtacgc
     5101 caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt gctcaaccac agaacttctt
     5161 cttcacccgc aggtttccac gagagaaggt gtgcgccctg taatgcaaaa gaggctttta
     5221 cctggggatg atcgaccaca atgaggtcca gttcatccag tttacgacgg gagaggacag
     5281 gggagatttg ttcgatgacc ggaagggcaa aaattttctt aatcatgatg cagtccttta
     5341 acttcatttt atcaggtaaa aaaaagagcg accgaagtcg ctctttttag atcacagtgt
     5401 catctcaact tatttggaga tgtgagcgat caggtccaga actttgttgg agtaaccggt
     5461 ttcgttgtcg taccaggata ccagtttcac gaagttgtcg ttcagagcga taccagcttt
     5521 agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg gtagatacta cgtcatcttc
     5581 ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca gctttaacgg cagctttgat
     5641 ctgctcgtaa gttgcagctt tttccagacg aacggtcagg tcaactacgg atacgttcgg
     5701 ggtcggaacg cggaacgcca taccagtcag tttgccattc agttctggca gtactttacc
     5761 tacagcttta gcagcaccgg tagaggacgg gatgatgttc tgggaagcgc cgcggccgcc
     5821 gcgccagtct ttgtgagacg ggccatcaac ggttttctga gtagcggtag tagcgtgaac
     5881 ggtggtcatc agaccttcga tgatgccgaa gttatcgttg ataactttag ccagcggagc
     5941 caggcagttg gtggtgcagg aagcgttgga aacgatgtcc tggccagcat atttgtcgaa
     6001 gttagcgcct ttaacgaaca tcggagtgtt gtctttagac ggaccagtca taaccacttt
     6061 cttcgcacca gcggtgatgt gtttacgagc agtttcgtca gtcaggaaca gaccagttgc
     6121 ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg ttagccggat cacgttcagc
     6181 ggtaacacgg atttttttac cgttaacgat cagatgaccg tctttcactt caacggtacc
     6241 gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat gccatgtaat cagcgtctaa
     6301 caggtcgttg attgcaacga tctcgatgtc agaacgtttc tgagcagcac ggaaaacaat
     6361 gcgaccgata cggccaaaac cgttgatacc tactttgata gtcatatatt ccaccagcta
     6421 tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa ccttacgcag cgtcaagcgg
     6481 aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa gcattacgcg actgactcgc
     6541 ctcactcttc ctttgggctt gagaccacat ggggtcggcg ccccgaattt taaagggcaa
     6601 ttacgataaa aatgtgattt tcatcacgat ttaggtgcag ccatttcgtc gagatcaagt
     6661 ttaggacaaa attttgccag ttggtgttaa tgttttgtta gaatcagtca cgtaatgtga
     6721 gcacgattaa agtgagatgt gagtaaatgg ctaataaacc ttcggcagaa gaactgaaaa
     6781 aaaatttgtc cgagatgcag ttttacgtga cgcagaatca tgggacagaa ccaccattta
     6841 cgggtcgttt actgcataac aagcgtgacg gcgtatatca ctgtttgatc tgcgatgccc
     6901 cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg ctggcccagt ttctacgaac
     6961 cggtgagtga agaatccatt cgttatatca aagacttgtc acatggaatg cagcgcatag
     7021 aaattcgttg cggtaactgt gatgcccatc tggggcatgt ctttcccgac gggccgcaac
     7081 caacaggcga acgttattgt gttaactctg cttctttacg cttcactgat ggcgaaaacg
     7141 gcgaagaaat caacggttga aaaaacgatt cagcaactta ttccacagga gcggaaaaat
     7201 tatgaatctt gatgacatta tcaacagcat gacgcctgag gtataccagc gtttgtcgac
     7261 cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg accgaggaac aaaaagagaa
     7321 ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat accgaagcac agcatatgac
     7381 aattgacaca aatggtcaga tggtcatgaa gagcaaacag cagttaaaag aagattttgg
     7441 tatctcagca aagcctattg ccatgtttaa gtaagtggtt catccccttt ttgccgcggc
     7501 tatgaagggg atagccaaca atgaccccac gactcgtctc aattatctcg acccgacctg
     7561 gctaagtccc agtccgatct cttgcagtcg cgttcacgcc tgaatgactt ctcctgaact
     7621 taagtctcag acctatttgg ccggtaatcc ctctcgaatt tgctcttttt gcgtcccgcc
     7681 ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa ttgtgatcta agttgtaaaa
     7741 cgcaggtcat tatctgtgtt atgcagattt aatgttgacc tcaaaaatca tgtgggtaag
     7801 tatgatggcc atctaagcaa aacaaaacac agaggatgac gcaatgaaag cactggctcg
     7861 gtttggcaag gcctttggcg gctacaagat gattcatgtc ccacaaccca tttgtggccc
     7921 ggaagacgta gtgattgaaa ttaaagccgc ggcaatctgc ggcgcagaca tgaagcacta
     7981 caatgtcgat agcggttctg atgagtttaa ctctatccgc ggccatgagt tcgcaggttg
     8041 tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg gggcaacgcg tcgtgtcgga
     8101 taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa cagggtgatt ttatgtgttg
     8161 tacagaaaaa gtaaaccttg gtctggataa caatacctgg ggcggtggtt tttccaaata
     8221 ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat gcgttgtggg aaatccctga
     8281 tggtgttgat tatgaggacg cagccgtact tgaccctatc tgtaatgcct ataaatccat
     8341 cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc gtcattggca ctggcccact
     8401 cgggctgttt tccgtacaaa tggcgcggat tatgggggcg gtaaatatcg tcgtcgttgg
     8461 tctgcaagaa gatgtggtgg cgcgctgcca gcaaatttgt ggcaaagata atctgggact
     8521 ggtgattgaa tgctccggtg ccaatatcgc actgaaacaa gccatcgaaa tgctccgccc
     8581 gaatggggaa gtggtacgcg ttggaatggg cttcaaacct cttgatttct cgattaatga
     8641 cattaccgcc tggaataaaa gcatcattgg gcatatggcc tatgactcca cctcatggcg
     8701 taacgctatc cggctattag ccagcggcgc tatcaaagtc aaaccgatga tcacgcatcg
     8761 tatcggcctg tcgcaatggc gcgaagggtt tgatgcgatg gtcgataaaa ccgcaatcaa
     8821 agtgatcatg acttacgact ttgatgaata actactctcc tgaaggaacg ttatcgcatg
     8881 gaacagataa caaaaccgca ttgtggtgca cggctggatc gcttaccgga ttgccgctgg
     8941 cattcgtcaa tgtttgccat cgtcgcgttt ggcttgcttg tctgctggag taatgccgtt
     9001 ggtggcttga tcctcgcgca gctgaaagcg ttgggctgga cagataattc caccacagcc
     9061 acattctcag caatcacgac cgccggaatg tttctcggtg cgctggtggg cggcatcatt
     9121 ggcgacaaaa ccggtcgcag aaatgcgttc atcctctatg aggccattca tattgcctcg
     9181 atggttgtcg gtgctttctc accgaatatg gattttctta ttgcctgccg ttttgtgatg
     9241 ggcgttgggc tgggagcttt actggttaca ctgtttgctg gtttcaccga atatatgccc
     9301 ggtagaaatc gcggaacgtg gtccagtcgg gtttccttta ttggcaactg gtcatatccg
     9361 ctctgctcac tgattgcgat gggactcacg ccgctgatta gtgcagagtg gaactggcgg
     9421 gtacaactgc ttattcctgc aatattgtcg cttatcgcta cggcgctggc ctggcgctac
     9481 tttcctgaat ccccgcgctg gctggaatcg cgcggacggt atcaggaagc cgagaaagtc
     9541 atgcggagta tagaagaagg cgtcatacgc cagacgggta aacctttgcc gcctgtggtt
     9601 attgctgatg acggtaaagc gccacaagcg gtgccgtatt cagccttact gacaggagta
     9661 ttactgaaac gcgtgatatt aggctcttgt gtgctgatta ccatgaacgt tgtgcagtac
     9721 acactaatta actggttgcc aacaatattc atgacccagg ggattaattt aaaagactcg
     9781 attgttttaa ataccatgag tatgtttggc gcgccatttg gtatttttat tgccatgctg
     9841 gtgatggata aaattccgcg taaaacaatg ggtgtggggc tattaatcct gattgcggtg
     9901 ctcggatata tctattcact gcaaaccagt atgttgctca taacgctgat tggtttcttc
     9961 ctgattactt tcgtctatat gtacgtttgc tatgcctcgg cagtgtatgt ccctgaaatc
    10021 tggccgacag aggccaaact ccgtggctcc ggtctggcga atgcggtagg gcgaatcagt
    10081 ggtattgccg caccttatgc cgttgcagtg ctgctcagta gttatggtgt aacgggagtc
    10141 tttattcttc tgggggctgt ttcaattatt gtcgcaattg ctatcgccac cattggaatt
    10201 gaaaccaaag gtgtctccgt tgaaagttta agtattgatg cagtagccaa taaataatac
    10261 gggtggattc aaaatgaaaa attcaaaagc aatattgcag gtgccgggca caatgaaaat
    10321 tatttcagca gaaataccag tgcctaaaga agatgaagtt ttgattaaag tagaatatgt
    10381 cggtatttgt ggttcagatg tacatggttt tgaatcaggc ccgtttattc cgcctaaaga
    10441 cccaaatcaa gaaattggcc tgggtcatga atgcgccggg acggttgtgg ctgtgggaag
    10501 ccgcgtgcgc aaatttaaac cgggggatcg ggtaaatatc gaacctggcg ttccttgcgg
    10561 tcactgtcgt tactgtctgg aaggcaaata taacatctgc ccggacgttg attttatggc
    10621 gacacaaccc aactaccgcg gcgcattaac gcactatctg tgtcatccgg agagctttac
    10681 ttacaaactg cccgacaata tggacacgat ggaaggggcg ctggtggagc ctgccgcagt
    10741 cgggatgcat gccgcgatgc tggcagatgt taaaccgggt aagaagataa ttattctggg
    10801 agcaggttgt attggtttga tgacgttgca agcgtgcaaa tgcctgggcg caacggaaat
    10861 tgccgtcgtt gatgtactgg aaaaacgtct ggcaatggcg gaacagcttg gtgcgacagt
    10921 ggttattaat ggcgcaaaag aggacactat tgcacgctgt cagcaattta ccgaagacat
    10981 gggcgcagat attgttttcg aaacagcggg ttctgcggtc accgttaaac aggcacctta
    11041 tctggtaatg cgcggcggta aaattatgat tgttggtact gtacccggcg attcggcaat
    11101 caatttcctc aaaatcaatc gcgaagtcac tatccagacg gtattccgct atgccaatcg
    11161 ttatccggtc acgattgaag ctatttcttc agggcgattc gatgtgaaat cgatggtgac
    11221 gcatatttac gattatcggg atgtacaaca ggcatttgaa gagtcagtta acaacaaacg
    11281 cgacattatt aaaggcgtta ttaaaattag cgattaatta atacgggaga acagaacatg
    11341 ctcgcagata tcagatattg ggaaaacgat gccacaaata aacattatgc aattgcccat
    11401 tttaacgtat ggaatgcaga aatgttgatg ggcgttaaag acgcagccga agaagcgaaa
    11461 tccccggtta ttatttcttt tggtacaggt tttgtcggta acacctcatt tgaagatttc
    11521 tctcacatga tggtatcaat ggcacaaaaa gcaacggtgc cggtaataac tcattgggat
    11581 catggtcgga gtatggagat tattcataac gcctggactc atggtatgaa ttcattaatg
    11641 cgtgatgctt ctgcatttga tttcgaagaa aatattcgtt taaccaaaga ggctgttgat
    11701 ttctttcatc cgctgggtat tccggtagag gcggaattag ggcatgtcgg taatgaaacc
    11761 gtttatgagg aagcgctggc gggctatcac tataccgatc ctgaccaggc ggctgaattt
    11821 gttgaacgta cgggctgtga ctcattggct gtcgccatcg gcaaccagca tggggtttat
    11881 acgtcagagc cgcaattgaa ctttgaggtc gtcaaacgcg tacgcgatgc tgtttctgtt
    11941 ccgctggttt tgcatggcgc atcggggatc agtgatgccg acattaaaac tgcaatttcg
    12001 ttgggtattg cgaaaatcaa cattcatacg gagctctgtc aggccgcaat ggtagcagtg
    12061 aaagaaaacc aggatcagcc tttcctgcat ttagaacgtg aagtacgtaa agctgtaaaa
    12121 gaacgggcat tggaaaaaat tgaactgttc ggttctgatg gcaaagcgga ataatgatat
    12181 ggataatctc gacgttattt gtataggtgc cgctattgtt gatattccat tacaaccggt
    12241 cagtaaaaat atctttgatg tagattctta tcctcttgaa agaatcgcaa tgagcaccgg
    12301 gggcgatgca ataaatgaag caacaattat ttctcgtctg gggcatcgta ccgcattaat
    12361 gagccgcatt ggtaaagatg cctcagggca atttattctc gaccattgtc gcaaagagaa
    12421 tattgatatt caaagcctga aacaggacgt cagcatagat acatctatta acgttggact
    12481 ggtgacggag gatggcgagc ggacgtttgt cacgaaccgt aacggcagtt tgtggaaatt
    12541 aaatattgac gatgttgatt ttgcgcggtt tcctcaggcg aaattattat cactggccag
    12601 tattttcaac agtcctctat tggat
//
LOCUS       DBKEKI010000115        12619 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.115, whole genome shotgun sequence.
ACCESSION   DBKEKI010000115 DBKEKI010000000
VERSION     DBKEKI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.115"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..178)
                     /locus_tag="ACUQPX_003164"
     CDS             complement(<1..178)
                     /locus_tag="ACUQPX_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HHV9057782.1"
                     /translation="MKSMDKMSTGIAYGTSAGSAGYWLLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFK"
     gene            complement(295..370)
                     /locus_tag="ACUQPX_003165"
     tRNA            complement(295..370)
                     /locus_tag="ACUQPX_003165"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(335..337),aa:Thr,seq:tgt)
     gene            complement(376..450)
                     /locus_tag="ACUQPX_003166"
     tRNA            complement(376..450)
                     /locus_tag="ACUQPX_003166"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(415..417),aa:Asn,seq:gtt)
     gene            complement(711..1334)
                     /locus_tag="ACUQPX_003167"
     CDS             complement(711..1334)
                     /locus_tag="ACUQPX_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HHV9057783.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGLAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAGTR"
     gene            complement(1331..1519)
                     /locus_tag="ACUQPX_003168"
     CDS             complement(1331..1519)
                     /locus_tag="ACUQPX_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HHV9057784.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(1516..1878)
                     /locus_tag="ACUQPX_003169"
     CDS             complement(1516..1878)
                     /locus_tag="ACUQPX_003169"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HHV9057785.1"
                     /translation="MNEYRFTLPYPPSLNTYWRRRGSRYYISDKGQKYRKDVQQIIRQ
                     LKLDIFTKARLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            complement(1875..2645)
                     /locus_tag="ACUQPX_003170"
     CDS             complement(1875..2645)
                     /locus_tag="ACUQPX_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HHV9057786.1"
                     /translation="MNAVIIANAEIVPIEFNGERVVTYQMIADIHGVPVATIQNGYAR
                     NSEYFIDREDTYFIDFHENTFRRGFEIPPRGMRVFTESGYLMLVKTLRDETAWRVQRE
                     LVRNYFRGQKEDKSALPDFTNPAEAARAWANEYEQKVKLEQQLAIAAPKVEFADRVGE
                     ASGILIGNFAKVVGIGPNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVN
                     TNHGIQISFTTKITGCGQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(2660..2896)
                     /locus_tag="ACUQPX_003171"
                     /pseudo
     CDS             complement(2660..2896)
                     /locus_tag="ACUQPX_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            complement(2971..3651)
                     /locus_tag="ACUQPX_003172"
     CDS             complement(2971..3651)
                     /locus_tag="ACUQPX_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HHV9057787.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYGTEPVRIRQNHHEN
                     KVRFVEGKHFFKVVGNDLKELRVALNYSQNPVSPKARSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFSQTSKSAGHHEKKTSELSAKDANSLVWLWDYANRSQALFRELYPA
                     LRQIQSNYSGRCHDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSAWVRLKNKE
                     LPPSLHHY"
     gene            4072..4302
                     /locus_tag="ACUQPX_003173"
     CDS             4072..4302
                     /locus_tag="ACUQPX_003173"
                     /inference="COORDINATES: protein motif:HMM:NF017757.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HHV9057788.1"
                     /translation="MKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRI
                     IMLHSFVKKTQKTPPAERKKAETRMKEVKHDW"
     gene            4292..4600
                     /locus_tag="ACUQPX_003174"
     CDS             4292..4600
                     /locus_tag="ACUQPX_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9057789.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4642..4818)
                     /locus_tag="ACUQPX_003175"
     CDS             complement(4642..4818)
                     /locus_tag="ACUQPX_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HHV9057790.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKCKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4815..5342)
                     /locus_tag="ACUQPX_003176"
     CDS             complement(4815..5342)
                     /locus_tag="ACUQPX_003176"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HHV9057791.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLIDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            complement(5339..5779)
                     /locus_tag="ACUQPX_003177"
     CDS             complement(5339..5779)
                     /locus_tag="ACUQPX_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HHV9057792.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            complement(5857..7732)
                     /locus_tag="ACUQPX_003178"
                     /pseudo
     CDS             complement(5857..7732)
                     /locus_tag="ACUQPX_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
     gene            complement(7962..8738)
                     /locus_tag="ACUQPX_003179"
     CDS             complement(7962..8738)
                     /locus_tag="ACUQPX_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9057793.1"
                     /translation="MSMNLMAKAMNIKVGNPLRKLILIKLADNANDNGECWPSYQHVA
                     DQCEVSRSTVKSHIRALEEMGLLKRECRRKGELNQSNVFYLTLDNAQQIPPESGGAGA
                     ARGGAGAALGGGAGAAPRTYHSFEPVNEPKNIMFEHVRTECEKTPDRHEETDKAFEEI
                     FWCAGMRKAGKKNAASAFRTQFREWRKTTRGTASEFATMLADDIACRNGKQFGFDRLL
                     PSSYLNGQRWNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
     gene            complement(8901..9200)
                     /locus_tag="ACUQPX_003180"
     CDS             complement(8901..9200)
                     /locus_tag="ACUQPX_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HHV9057794.1"
                     /translation="MTQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYAIDGLTNKKRPVCKTERSEQIQMEF"
     gene            complement(9339..9563)
                     /locus_tag="ACUQPX_003181"
     CDS             complement(9339..9563)
                     /locus_tag="ACUQPX_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HHV9057795.1"
                     /translation="MNLVIQRALKIAGSQKRLADKCGVTQPAVHKWLKGGQVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            9675..10382
                     /locus_tag="ACUQPX_003182"
     CDS             9675..10382
                     /locus_tag="ACUQPX_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HHV9057796.1"
                     /translation="MKTFAERLNAAMRSAGVSQSQLADMVGISQPAIQKMSSGKTNGS
                     RKMVELANALKVRPEWLSSGIGEMRDGTHEEPSNIRESSLKAVVWEDIKKNDDEFVAL
                     PLLNVSLSAGSGSCEIEESSEFSLVFRKYYLKKMGVTESSAKLVRVTGQSMEPTLHDG
                     DVVGVNTQDTTIRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEF
                     HKTVRIIGRVFWSSHSW"
     gene            10420..10761
                     /locus_tag="ACUQPX_003183"
     CDS             10420..10761
                     /locus_tag="ACUQPX_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HHV9057797.1"
                     /translation="MAFTDLEYQAVKKEVHQFIESIRPPEHIRNELDIVYSINDQTID
                     IGEQRPVWQGNPGETNILPSARIKYIRSLDRWKIYWMRKDMKWHQYSTELSLTDALEL
                     VRADPDCCFFG"
     gene            10951..11484
                     /locus_tag="ACUQPX_003184"
     CDS             10951..11484
                     /locus_tag="ACUQPX_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057798.1"
                     /translation="MQTKSYTIKAFQDFLQMLPELGIVNDATARNLRDSSLRLLTVLG
                     SDFTDADIRDYSVANLASSYADSAESKPSESSLQAYKSRMQSAIDKFISYQNGEINVS
                     VGDVNKKKERKKMAPKKKAVADVQVGVKTFELPIPLRGDLIVTIGNLPRDLTKDEAKR
                     ISLIVESFAMIDGGTKE"
     gene            11658..11792
                     /locus_tag="ACUQPX_003185"
     CDS             11658..11792
                     /locus_tag="ACUQPX_003185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057799.1"
                     /translation="MAHDVNRDDFIYEHDKPVYVRSYCRIRFGELEHVRQHFRSYPSC
                     "
     gene            12289..>12619
                     /locus_tag="ACUQPX_003186"
     CDS             12289..>12619
                     /locus_tag="ACUQPX_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HHV9057800.1"
                     /translation="MTRRTQFKGNSRSRRRERLKAKALANGVLAREEAISSEVLHRPT
                     LSRAQIQAKGTHETPERIEDAKPIKFMAQDVIWQQKEYRRNLERAAIVYANEFGHKQP
                     ETGVCLPN"
BASE COUNT         3162 a         3143 c         2894 g         3420 t
ORIGIN      
        1 ttttgaaata cagatttgtc agataagtca gaagccccag aagcagactt cccagcacac
       61 caatcgcagc ccactgtgac ggactgacct gatcaagcca ctgtaaaagc cagtagccgg
      121 cactgccggc ggaggtgccg taggcaatgc ccgttgacat tttgtccata gatttcataa
      181 cctcacctcc gcaaataacg gatggcgtag ttcttatatt gggaagggga aaagaaggcc
      241 gcagcgtaac tgtcactgat gaattcagga tagccagtgg ctacggctca gttatggtgc
      301 tggttaacgg acttgaaccg ctacccattc gcttacaagg cgactgctct accattagag
      361 ctaaaccagc atatttggcg ggacagcgtg gactcgaacc acgataagaa ggttaacagc
      421 cttccgtaat gacctttata cgactgaccc aaataaaaaa tcccgaaagc aggctctatt
      481 cctatgtgct ctcgagttta tgatgcgcat gtcagtgcgc ttagcgaaaa acgccgtaaa
      541 ccctcgcccg acagggcggg gagcagtcac aacttaaaga tgaggtgggt aaaaaaccga
      601 aagaatgtga acggatataa acctgccatt cttgagtcaa atttacccaa ctttattcaa
      661 aaagtcaata tcatgccgtt aagatgttgc catccgtggc aatcatgccg ctaacgtgtg
      721 cccgcattca aaatgttgtc tgcgattgac tcttctttgt ggcattgcac caccagagcg
      781 tcatacagcg gcttaacagt gcgtgaccat gtgggttggg tgaggtttgg gattagcatc
      841 gttacagcgc gatatgctgc acttgctggc atccttgaat agccgacacc tttgcatctt
      901 ccgcactctt tctcgacaac tctcccccac tgctctgttt tggctatatc aactgccaga
      961 cctgttccgt ggcaatctct gcatcttgcg cccggcgtcg cggcactacg gcaataatcc
     1021 gcataagcga atgttgcgag cacttgcagt acctttgcct tagtatttcc ttcgagcttt
     1081 gccacaccac ggtatttccc cgataccttg tgtgcaaatt gcatcagata gttgatagcc
     1141 ttttgtttgt cgttctggct gagttcatgc ttaccgcaaa atgcagccat tccgaatccg
     1201 gcttgtgatt gcgccatccc catagcagcc atcacatcag taccggaaag agagtcagaa
     1261 gccgtagccc gtggtgagtc gctcatcatc gggctttttg gcgaatgaaa tttagctacg
     1321 ctttcgagtc tcatcgtctt cccctcttgc cctgtttgac catcaggacg ccgttaacta
     1381 ttacgtgacg ctcgcctttg ctgtctcggt tgtacttgag cactgttcct cttgcgcagg
     1441 aaagcatcct cgccacttcg gtctgattgc ctcgtgtctg gataagaagc tctggtatcg
     1501 tttgaattgt ggcgttcata cgttctccag ttcggtgatt tttattccaa gccttccgcc
     1561 tggtactttc acaccacgaa ttacgcgaat gtcatcgaat tgctcgtcgt cttccgcaaa
     1621 tccggcgtgg ataagagagt cgagtagccc cttcaggatg ttatcgaggt cgcggcggcg
     1681 ggagtctgga acgtctgcga tgactttgat gcggagtcgt gctttggtga aaatatctaa
     1741 cttgagttgg cggatgattt gctgaacgtc ttttcggtat ttctggcctt tatcgctgat
     1801 gtagtatcgg cttccccgtc ttcgccagta ggtattcagc gatggtgggt atggaagcgt
     1861 gaatctgtac tcattcatgc agcctcccct gttactttca gcattccgtt atcgagcagc
     1921 tttctggtca gccactgttg gccacacccg gtgatttttg tggtgaacga tatctgtatt
     1981 ccgtgatttg tgttgactgc tgtttctttc actgtgaaat agccgcgatc catatattcc
     2041 tgcattggca cattgcgccg ggaacctgaa gcaataagga ttttgtgatc acgcatccac
     2101 gcaaacagtt tgtttgggcc aataccaaca acctttgcaa agtttccaat caaaattccg
     2161 ctggcctcgc caacgcgatc ggcaaactca actttaggtg cggcaatcgc gagttgttgc
     2221 tccagcttca ctttctgctc atattcgttt gcccacgctc tggcggcttc agcagggttt
     2281 gtgaagtcgg gcaaggctga cttatcttct ttttggccac gaaaataatt gcgaacaagc
     2341 tcacgttgca ctctccacgc cgtctcgtcg cgcagggttt taaccagcat caaatagcct
     2401 gattcagtga aaactcgcat gccacgagga ggaatttcaa aaccccttct aaaggtattt
     2461 tcatgaaaat caataaaata tgtatcttcc ctgtctatga aatactcaga gtttcgcgca
     2521 tagccatttt gaatagtggc tactggaacc ccgtgaatat cagcaatcat ttgatacgta
     2581 acaacacgct cgccattaaa ctcaatcggc acgatttctg cgttggcaat aattacggca
     2641 ttcatgcaac acctccttca acgacaataa tgctgtcgcg cttaccttgt tcgccttcga
     2701 agacgtaatg ctcgtactga acattgaacc ctaagttatt gattctttcg gaaacctcaa
     2761 attgaggatg ccggataaca ccatttttag ccagcgtttc gatggtacgt ttcacattgt
     2821 catgacgctt acccaccaac tcagcgattt caatgctggt cattttgatg gcgttgctat
     2881 ttatcagctc gttcattgtc atgtcctctc atattgaaaa ttcagcaata aaaaacccag
     2941 ccgaagctgg gtcgttgcgt tggcaatctg tcagtagtga tgtagtgaag gaggtaattc
     3001 tttgttctta agtcttaccc atgcagaaag attcgttggt ccgtctggct cattaatatc
     3061 aacatctcgt gtgtgattga ttaaaacgtc tctcgccatt ccgataacat acgagaactc
     3121 atgaccgtag tcgtggcatc tgccggaata gttagactga atttgtctta gcgccggata
     3181 cagttcgcgg aataatgcct gtgagcggtt ggcataatcc cacaaccata ccagactgtt
     3241 tgcatctttt gcagaaagct cgctggtttt cttctcgtga tgacctgcgc ttttcgaagt
     3301 ctggctgaaa tagcagtctt ccagtttttc gaacacttcc cacgcctgat cggtttcgag
     3361 cattttggcg tggcgtgcgg ctccgcgttc tgtccagagg atgagggagc gggctttggg
     3421 tgaaactgga ttttgtgagt agtttaaagc gacccgcaat tctttaaggt cattaccaac
     3481 aactttgaaa aagtgtttcc cttcaacgaa gcgtactttg ttctcatgat gattctggcg
     3541 aatacgcacc ggctcagtgc cgtaaagctg cgccaaaagt tcggtggtaa taacaggaat
     3601 ctggttatgg gtgatcgggg agagagtttc aacagagatt tgagtggtca taacgataac
     3661 tccgtacatt tggtcattat cgccaccttc tggtctcaat catctggtgg cgagctgtgc
     3721 agggttgaga ctaccgggta cggaaaccgg cgagcctttc ggctccccca cacagcccac
     3781 cataaatcgc gaatgtgact gtgcaaacga tatgaaaaaa gacgcgggcg cgtctcatat
     3841 cgctccgtaa acatccgggg tctcaatccc gacgccagat tttgctggcg tatgaggaat
     3901 atagccccgg atatgtgttg tcgtcaacta caggccttga atgcatataa cgattttgtt
     3961 atgttgatac ctatgaacta cactatcgaa tactacagtg aagatgtaag gctggaagtc
     4021 gatcggcttc cattgagtat gcgtgcctga taccaacatc tcgttgaacg tatgaagata
     4081 tatggcagca atctcggaga acctcacacc agcgcctttg gtgacgggct tttcgaactc
     4141 agaattaaag gcaacgatgg gatcgcgcgt gttttttact gcactctgac agggaaacgc
     4201 atcatcatgc tgcatagttt tgtaaagaaa acgcagaaaa caccgccagc cgaacgcaag
     4261 aaagctgaaa ccagaatgaa ggaggttaag catgactggt aaacgcactc tccccaccat
     4321 gacacacgac gaaatggcag ccaaatggat ggaagacccg gcttttaaag cagaatacga
     4381 cgctatcgct gacgaattcg cactgcttga tgaaatgctg gcagcacgca aagaagctgg
     4441 cttaactcag gctgaagttg ccgagcgaat gggaacaaaa gcgaccgcga tcaccagaat
     4501 ggaaagtaat ctcgcatcag gtatcagcgg cccatcattt gccaccctaa aaaaattcgc
     4561 ccgcgctact ggaaaaaaac tccagatccg cttcgtttaa ccacaccgcg ccgtcattct
     4621 ggcggcgcac cggataaata gtcatttcct cgcacgatgt cttagccacc ggatatccca
     4681 caggtgagcc gtgtagttga aggtttttac gtcagattct tttgggattg gcttgcattt
     4741 atttctggag cgtttcgttg gaaggtattt gcagttttca cagatgatgt cggtgaaact
     4801 tcgtcgctgt cgcctcatgc tgccctcctg acgccctgcc cgatcgccat caatgccgct
     4861 ttggatacgg tagtaaacat ccgtcgagga ctgatgaacg gtcgccaaat cagcagcatg
     4921 gagcctttgc tgttgccctt cttctccagc cctgtcgatg gttcgataaa attaatccgt
     4981 ccatcagtga taatgcgaac ttcgtcgaca ctctccagag ccttgctgaa ccatccgact
     5041 gacatatcct ctggcacaag cataactacc gtctgtcgct gttgtatgca ctgctcagcg
     5101 gctttttcca cccacggcct gatattgctg tacggtgggt tattccagat tgctccgtgg
     5161 cttacccact cagaattgag cgcgtcgtcg gcctcagtta gccagtgagc gcacagagca
     5221 tttttgtcgc tcgctgccga atccagccag aatccaaact caatatccag cgcatcaata
     5281 agccaaagtg gcgtttgcca gcagtccttg tcgtgtgctg gcgtatttga tttgatagtc
     5341 atgcagcccg atctccccat cgcgctttcc actccagagc cagtcgcgct tcgtctgacc
     5401 acttaacgcc acgctctgta ccgaatgcct gtataagctc taatagctcc gcaaattcgc
     5461 ctacacgcat cctgctggtt gactggccta ttaccacaaa gccattcccg gcaaggttag
     5521 gaacaacgtc ctgctgcttt aatgctgcgg taaacacaca cttccagctt tctgcatcca
     5581 gccagcgccc atgccattca acctgacgag agacgtcacc aaggcaagcc caaagctttc
     5641 gattctggtc taagctgcgg ttgcgctcct gaatggttac tacgattggt ttggttgggt
     5701 ctggaagaat ttgctgtacc gcgtgaatag cgttttgctg atgtgctgga gatcgaattt
     5761 caaaggttag ttttttcatg acttccctct cccccaaata aaaaggcctg cgattaccag
     5821 caggcctgtt acaagctcag tgatgtagat ggtcatttaa tactccgtca cgttttcctg
     5881 tcgccacgcc tcgtcatatt ccgatttcgg catattggcg atgtagctat atggcgatcc
     5941 tgattcaagt tgcaggaact ggtgcgattg ctcgtcaagg aacaacggga caccaccttc
     6001 ccaaccttcg cagttacgtt gtttttcaag catcaaaaca gatgccggag atgccagtag
     6061 ctgttcgtcc ttctctgaca tcttttcacc actctgaact ctctgtaacg ctctctcgcg
     6121 agtcttgtta cgccagatga tgaaaaggtt gtctgtcagg tctgttatcg ctccagagcc
     6181 ttttacgtcc attttcccgg ttggtttttc ttcgctgtct ccttttcgcg agtgagtaac
     6241 gagaatgacg tgggagtttg ttttgttttt gaagtcgcaa atcgagtcaa caaacgcctt
     6301 ctgcccgtta tagtcatcgt cgcctatgcc acatttcatc aggctgtcga tgatgaataa
     6361 ctggatcccg tatcggcggc gagcgtagtc gaatatttcg atcagcctgt cggctttcgc
     6421 cgttccggtc aggccaaaca cccaaagtct ttcgtcataa aatttaaatg cagagtcaat
     6481 ttccagcact ggcggaatct tgcagcacgt cgcctgacgg gtaaggcgct taaggagaat
     6541 accaggcttc agctcaagtg acgcgatgca cgtcttcaca ccctgacgca ttgcctcaag
     6601 tgccatatgc ccgacaacct ccgtttttcc gtgaccgttc acaccattga ccagcgtcaa
     6661 ctctgcctca cggaactaga atttatctgc cagagattcc cacggtggat taaacagata
     6721 ctgctgcttg ccgtagaaag cgttgatagt gtcctggtaa aaccgcactg tagagttctt
     6781 caggatcgaa gtaggatgcc gtgccgatgt actgccagat ttcatcctcg gtaacaccgt
     6841 tcatcaggca ttcgttgatg tctttgtacg gcagagtaac aagacggcaa cgatgttcac
     6901 cgagtcggct tgcgatttcc cttgcggctt cacgaccaac atcatcaacg tccatcgaga
     6961 tgaatatttc ctcaaacctg tcgaggttgt gatactcaaa ctcaatccac tgttgcttag
     7021 cgcctttccc gccaccaaac ggcacggata acgccgagat gccgtattgc gcatagctca
     7081 tacaatcaat ttcgccttcg caaagcacaa ccgccctcac gccagcgtcc agagcctgcc
     7141 atccgaacag gcaaggttcg caatcacctt ctgccataat gactttcttc ccgtccgggc
     7201 gctcagtgct gattcgcttg acctgcaaca actcaccatc gcgtttgtac ggaagcacca
     7261 gagcatccag ttctcgttct ccattccaca ccttgccgct gacaacctcg tagcgcttta
     7321 cgacttctgg cgatatgcca cgcgattgca ggtactcaag atgggattct gttctggtaa
     7381 cgtagtgggc gattttcttg cgatcaggtc tggagaattt cttctcacgt ttggcatcga
     7441 aatggtgatc gtcatccttg ataccgagaa atgctttcgc ttcctgcata gcctgatgca
     7501 ggttaattcc acgacatgcc atccacaaat caagcatgtc accgccgtct ccctcagcga
     7561 aatcagccca ttttttctta ccgctaaggt tgaccttaag gctgtttccc ttgtcaccgt
     7621 tgacgttacc ggcaacccac tcatgcccct ctttcttgcc gtttggcaac aggtgcggag
     7681 ccaccctgtc aacctgcgcc caaagcaggt cgctaagttc tgatggactc atactttaac
     7741 cgtcctgaat cgcattctga cgcaatattt tttatcccgt tattttcccg tcagaccgtc
     7801 gtttatcgcg ctacagcgcg tctgatggcg tttaaacgcc attgacggag ctaatggctg
     7861 ctgaggtcga gatttttaaa ccagaaatcg acaaacgaaa tacttaacca gccgtggtta
     7921 taaccagcga ccagtagcga tttgattttt gatttcatgg ttcacctgtc gaaaaacacg
     7981 tagccagttt tcgatacggt aattgcggat gatggtctgg attgtggttg aatggtttct
     8041 ggcttctcgt cgttccagcg ctgaccgttc aggtagctcg acggtaacaa cctgtcgaat
     8101 ccgaactgct taccattcct gcatgcaata tcgtctgcca gcatcgtggc aaactcgctt
     8161 gccgtacccc tggtagtttt acgccattcc ctgaactgtg ttctgaatgc cgaagctgcg
     8221 tttttcttcc cggctttccg catgcctgca caccagaata tttcctcgaa tgccttgtcg
     8281 gtttcttcgt gacggtcagg agttttttca cactccgttc ggacatgttc gaacataatg
     8341 tttttaggtt cattgactgg ttcaaaagag tgataggttc tgggggcagc tcctgcccca
     8401 ccccctaggg cagctcctgc cccaccccgg gcagctcctg ccccacctga ttctggtggg
     8461 atttgttgtg cattatccag cgtcagataa aaaacgtttg actggttaag ctctcctttt
     8521 cttctgcatt cccttttcaa aagccccatc tcttccagtg ccctaatgtg actttttact
     8581 gtcgatctgc tcacctcaca ctggtcagcg acatgttgat atgaaggcca gcattcgcca
     8641 ttatcattgg cgttatcggc aagtttaatc agaatcagtt ttctcagtgg gttgccaacc
     8701 tttatattca tggccttagc cataagattc atgctcattt tgacttctcc gaagttttgt
     8761 acctgttaag tatttctctc agtggcacag ctatttctgg attaaccccc tgataaaact
     8821 ggtcacgtag cacatctttt cggtgattaa cgcgtttatt ttcctgcgtt tttcgcatat
     8881 aattacctcg ttggatgtta ttaaaattcc atctggattt gttcagaacg ctcggtcttg
     8941 cacaccgggc gttttttatt ggtgagtcca tcaatcgcat acttaaaagc cctgctaatc
     9001 ggactgatgt ctgatgccat tccgaaagca cacaagaccg aagcaataaa cctccagtcc
     9061 gttctgctta tcttcgattc atgacagcca atcatctttg ccagaccgcg ctgggtaagc
     9121 gttgacaggt tgatgagtaa atctgtttca gcgcgatcaa cgtcgcgctg tgatagtttg
     9181 ctgtaacttg tttgtgtcat ttcttaatat ttccagtagt gaatagttag ttgaaaggta
     9241 tgcatggaaa cgcatatggc cttagttggt cagatatctt ggggctcgct ttgtcagcga
     9301 cgtaggacga atgcccattg tgaaaatagc ggtgttactt atgctgcctg attcggtttt
     9361 ggaaacaggt gtggtaaatc tgggcgaatt tcgtaagcct tgatctgccc tccagtggcg
     9421 ttaacgatgg cggtaacttt ctctggagaa acctgcccgc ctttcagcca tttgtgtact
     9481 gctggctgcg ttacaccaca cttgtcggca agccgctttt gactaccggc aatcttcaag
     9541 gctcgttgaa ttactaaatt catgagcata cctctttcag ccattactta taaccaagga
     9601 taacccaagt tataaaagat ggcaataacc tttattattt tactttggat aaccgtagtt
     9661 atagatttgt ggatatgaaa acattcgcag aaagactaaa tgcagccatg agatcggcag
     9721 gggtgtcgca atcacagctt gcggacatgg ttggaatatc tcagccagcc atacagaaga
     9781 tgtcatccgg taaaacaaac ggatctcgca agatggttga gttagctaat gctttaaaag
     9841 tacgccctga atggcttagt tctggtattg gtgaaatgag ggatggtaca catgaagaac
     9901 catccaatat ccgtgagtca tctttaaaag ctgtggtatg ggaagacatt aaaaaaaacg
     9961 atgacgagtt tgtcgcattg cctcttctta acgtttcgct ttcggctgga agcggtagct
    10021 gcgagataga agaatcatca gagttctctt tggttttcag aaagtactat ctgaaaaaga
    10081 tgggggtaac tgaaagttct gccaagctgg taagggtaac aggtcaaagc atggaaccaa
    10141 ctcttcacga tggcgatgtt gttggtgtta acacacaaga taccaccatc agagatggta
    10201 aaacctacgc tatttgccag tctgatttgt tgcgagtaaa aacattaatc gccactccta
    10261 catcggtgat aatcagatca ataaatcgcg aagagtaccc ggatgaagta atggatagag
    10321 atgaatttca taaaaccgta aggattattg gcagagtatt ctggtcatcg catagctggt
    10381 aaccagtggc ctgaagaaac gtttgggtga ggaggataga tggcgttcac tgaccttgaa
    10441 tatcaagcgg tcaaaaaaga agttcaccaa ttcattgaaa gcataaggcc gcctgaacat
    10501 atccgcaatg aactggatat tgtttatagc atcaatgacc aaacgataga tatcggcgaa
    10561 cagcgccccg tgtggcaggg caacccaggt gaaacaaaca tcctgccatc agcaagaatc
    10621 aagtacatac gttctctgga tagatggaaa atctattgga tgcggaagga tatgaaatgg
    10681 catcagtaca gtactgaact ttcgctgact gatgcgcttg agcttgtgcg tgctgacccg
    10741 gattgctgct tcttcggatg agtgaagaga cattttggtg atggatggtc gcagaagtgc
    10801 ggcctttaat gtaatcgaag aaaagtaatc cttgtaaaac ttgatttgac tgtattgtca
    10861 caaagaaagg aatcaacgat cgtttcttgg gtcttgattt gttttgacga tcaaattagt
    10921 attgcctcat caactgtatg aggctttttt atgcaaacca aaagctacac catcaaagca
    10981 tttcaggact tcttacagat gcttcctgag cttggtatcg tcaatgatgc tacagcccgc
    11041 aatctccgtg actcttccct gcgccttctt actgttcttg ggtcggattt caccgatgcc
    11101 gatatccgtg attatagcgt tgccaactta gcaagttcgt acgcagactc agcagaaagc
    11161 aaaccctctg aatcatcttt gcaggcatac aaaagccgaa tgcaaagtgc tattgataaa
    11221 tttatctcct accagaatgg tgagattaat gtgtcagttg gtgatgtaaa taaaaaaaag
    11281 gagcgtaaaa agatggcacc taagaagaag gcggtagctg acgtacaggt tggtgtaaaa
    11341 acatttgaac tgccgattcc attgcgcgga gatcttatcg tcaccattgg caacctgcct
    11401 cgcgacctga caaaagatga agccaagcgt atcagtctga ttgtggaatc ttttgctatg
    11461 attgatggcg gaacaaaaga ataaagcccc gcggcaacgg ggcttttggg gtcggaaata
    11521 tttccagtat ttccgaagtg tatggaggat tacctaccgc tacacccttg gcaaccgcaa
    11581 gccaaggata gcggttctcc caacaaacta caacctgttc gggtaaacag gctgtataca
    11641 aaaacaggag aaaagctatg gctcacgatg ttaatcgcga tgacttcatc tacgaacacg
    11701 acaagccggt ttatgtgcgc agttactgcc gcatacgttt cggtgagctc gaacatgttc
    11761 gtcagcactt ccgctcttat ccgagctgct gaaaataact aaatctcaaa cccggcttcc
    11821 gcgccgggtt ttctttgcct cgcgatcgtc ccccatattt aacagccgca aatgcggtaa
    11881 accacgaacc aacacgccag caatacagcg ccccccttcc aataccgacc aatccgcaac
    11941 atttacaaaa ataaaatacc tttattatcc atcacttata acttatttat cagaatccat
    12001 aaattaagtt attgaccacc gctataacct aggttatctt taagccatca gcaggacgca
    12061 ctactcacca gggcggtgaa tatacagcga ttcgaatatg aatctacggc gctgacaaag
    12121 cgcaataacc aaagtgaact ttggggtgtg gtgaagggtt catggacggg aatatgtcgc
    12181 acgtaaagcg gcgaggcctg cgggactatt gccgaattga agtaggccga aacaggtcaa
    12241 aatgggtctc ccacctacca caccaccaaa gttcatcagg aggtccatat gacacgcaga
    12301 actcagttca aaggcaattc acgttctcgt cgtcgtgagc gtttaaaggc aaaggcatta
    12361 gctaacggcg tactggcccg cgaagaagca ataagttcag aagtattaca ccgccccact
    12421 ctaagcagag cgcagattca ggctaaaggt actcacgaaa cgcctgagcg catagaagac
    12481 gctaagccaa ttaagttcat ggcacaggac gtgatctggc agcagaaaga atacagacgc
    12541 aatctggagc gagcggccat tgtgtacgcg aatgagtttg gacataagca accagaaact
    12601 ggtgtatgtc ttccaaacg
//
LOCUS       DBKEKI010000116        12560 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.116, whole genome shotgun sequence.
ACCESSION   DBKEKI010000116 DBKEKI010000000
VERSION     DBKEKI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.116"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..793
                     /locus_tag="ACUQPX_003187"
     CDS             <1..793
                     /locus_tag="ACUQPX_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHV9057801.1"
                     /translation="ISGDAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPL
                     YQGMRDGKIVRFEEITRTPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIAT
                     ANTRDRGVNEMSAALKRRFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAV
                     LELLVHTFRDLRANGEKKTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLV
                     DCIAGTIVKDNEEDRARLRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            804..3083
                     /locus_tag="ACUQPX_003188"
     CDS             804..3083
                     /locus_tag="ACUQPX_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HHV9057802.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGVWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGNSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLAPFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            3076..4212
                     /locus_tag="ACUQPX_003189"
     CDS             3076..4212
                     /locus_tag="ACUQPX_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HHV9057803.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSIVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            4209..6208
                     /locus_tag="ACUQPX_003190"
                     /pseudo
     CDS             4209..6208
                     /locus_tag="ACUQPX_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
     gene            6333..6794
                     /locus_tag="ACUQPX_003191"
     CDS             6333..6794
                     /locus_tag="ACUQPX_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HHV9057804.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(6835..7305)
                     /locus_tag="ACUQPX_003192"
     CDS             complement(6835..7305)
                     /locus_tag="ACUQPX_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HHV9057805.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(7352..8071)
                     /gene="btsR"
                     /locus_tag="ACUQPX_003193"
                     /gene_synonym="yehT"
     CDS             complement(7352..8071)
                     /gene="btsR"
                     /locus_tag="ACUQPX_003193"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HHV9057806.1"
                     /translation="MIKVLIVDDEPLARENLCIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(8068..9753)
                     /gene="btsS"
                     /locus_tag="ACUQPX_003194"
     CDS             complement(8068..9753)
                     /gene="btsS"
                     /locus_tag="ACUQPX_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HHV9057807.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9975..10040
                     /locus_tag="ACUQPX_003195"
                     /pseudo
     CDS             9975..10040
                     /locus_tag="ACUQPX_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10268..10516
                     /locus_tag="ACUQPX_003196"
     CDS             10268..10516
                     /locus_tag="ACUQPX_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HHV9057808.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(10632..10901)
                     /locus_tag="ACUQPX_003197"
     CDS             complement(10632..10901)
                     /locus_tag="ACUQPX_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HHV9057809.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            10958..11626
                     /locus_tag="ACUQPX_003198"
     CDS             10958..11626
                     /locus_tag="ACUQPX_003198"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHV9057810.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            11941..>12560
                     /locus_tag="ACUQPX_003199"
     CDS             11941..>12560
                     /locus_tag="ACUQPX_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HHV9057811.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPASLEYINVSDNHLSVLPRLPMSLELLDAARNALEVIPDFPER
                     DDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQLSPRIMQTL"
BASE COUNT         3200 a         3201 c         3086 g         3073 t
ORIGIN      
        1 tattagcggc gatgccgggt taaccatcca gggcggcgca tctactaccg aagatcaaat
       61 caagtacggc tggaactacg ccctgcttat caatcatggt ccatcaacgg aagcgctggt
      121 ccctgccccg ctttaccagg gaatgcgcga cggcaaaatc gtccgttttg aagagataac
      181 gcgtacaccg ctggaagtgc aagactgtct gttaggtatg ctttctgaca gggtgatgac
      241 ggtgccggaa ctcactggtg aagccagtca gctctatgcg cgagaaggct ttaatattat
      301 tgccactgcc aatacccgcg atcgcggcgt caatgaaatg agtgctgcgc taaagcgtcg
      361 cttcgatttt gaaactgtct ttccgattat ggatttcgcc caggagctgg agcttgtcgc
      421 cagtgcttcg gcgcgtttgc tggcccatag cggtattccg cataaagtgc ctgacgcggt
      481 actggagtta ctggtccaca ccttccgcga tctgcgtgcc aacggcgaaa agaaaacgtc
      541 aatggataca ctaacagcga ttatgtccac cgctgaagcc gtgaacgtgg cccacgccgt
      601 aggcgtccgc gcctggtttt tagcgaatcg cgcaggtgag ccagccgacc tggtggactg
      661 cattgccgga accatcgtca aagataacga agaagatcgc gctcgtctgc gccgttactt
      721 cgaacagcgt gtcgccacac ataaagaagc tcactggcag gcttattatc aagcccgcca
      781 ccgcctgcca tgaggaaaga tgcatgagcg agccgttaat tgtcggcatc cggcatcata
      841 gtccggcctg cgcccggctg gtgaaatcgt taatcgaaag ccagcggcca cgatacgtgt
      901 tgattgaagg cccggctgat tttaatgacc gggtagacga actgttttta gcccaccagc
      961 ttccggtagc tatttacagt tattgccagt atcaggacgg tgcagccccc gggcgtggtg
     1021 tctggacgcc atttgctgaa ttttcgccgg agtggcaggc gctacaagcc gatcgtcgta
     1081 ttcaggcaca aacttacttc atcgatttgc cttgctgggc gcagagtgaa gaagaggacg
     1141 attcgcctga tacgcaagag gaaagccaga ccttgctgct gcgtgccacc cgcatggata
     1201 acagcgacac cctgtgggat cacttgttcg aagatgaaag ccagcaaact gcattaccct
     1261 ctgcactggc gcactatttt gcccaactgc ggggcaactc ccccggcgat gctctcaatc
     1321 gtcagcgcga agcctttatg gcccgctgga ttggatgggc gatgcagcaa aataatggcg
     1381 acgtgttagt tgtctgcggt ggctggcacg ctccggcact ggcaaaaatg tggcgcgaat
     1441 gtccgcaggt aattaacacg ccagaattgc cgtcgctggc agatgccgtt acaggttgtt
     1501 atctcacccc ctacagtgaa aagcgccttg atgtgctggc aggatacctt tcaggaatgc
     1561 ctgccccggt ctggcaaaac tggtgctggc agtggggctt acagcaggcg ggagaacaac
     1621 tgctaaaaac ggttctcacc cgtttgcgcc agcacaagtt gcctgcttcg accgcggata
     1681 tggctgccgc tcatctgcat gcgatggcac tggcacagtt gcgcggtcat acactaccgt
     1741 tacgcactga ctggctggat gccatagcag gatcgctgat taaagaagcc ctgaatgcgc
     1801 cgttgccgtg gagctatcgc ggcgttattc atcccgatac cgatccgatt ctgctaacgt
     1861 tgatagacac attagcgggt gacggattcg gtaaacttgc cccttctacg ccacaaccgc
     1921 ctctgccaaa agatgtcact tgcgaactgg aacgtaccgc aatctctctt ccggcggagc
     1981 ttaccttaaa tcgctttaac cccaatggac tagcgcaaag tcaggtgtta catcggctgg
     2041 caatactgga gatcccaggg attgtacgcc agcagggaag cacactgaca cttgcaggca
     2101 acggtgaaga acgctggaaa ttaacccgcc cgcttagcca gcatgcggca ttgattgagg
     2161 ccgcctgctt tggtgccaca ctccaggaag ccgcacgcca taaattagaa gccgatatgc
     2221 tggacgcggg tggaatcggc agcattacca catgtcttag ccaggcggcg tcagcgggtc
     2281 tggcgccctt cagtcaacaa ttactggagc aactcacatt attaatcgcc caggaaaatc
     2341 aatttgccga aatgggccag gcgctggaag tgctatatgc cttatggcgg ctggatgaaa
     2401 ttagcggtat gcaaggcgcg cagatattac aaacgacgtt atgcgcggct atcgatcgca
     2461 cgctgtggct gtgtgaatcc aacggcagac cggatgaaaa ggagtttcac gctcacttgc
     2521 atagctggca agcgttgtgt catatcctgc gcgatctaca tagcggtgtg caactccccg
     2581 gcgtttcgct ttctgcggcg gtagccttgc tggagcgaca cagtcaggcg attcatgtcc
     2641 tggcactgga tcgcggcgcg actcttggtg cgctgatgcg tctggaacat cccaacgcta
     2701 gtgccgaagc ggcgctgacg atgctggcgc agttatcccc ggcacaatcc ggcgaggcgc
     2761 tgcacggttt gctggcatta gcccgtcatc aactggcctg tcagccggca tttatcgccg
     2821 gtttcagcag tcatttaaat caactaagtg atgccgattt taccaatgcc ctgcccgatt
     2881 tacgcgcggc aatggcctgg ctaccgccac gagaacgcgg gacgctggcg catcaggtgc
     2941 ttgagcatta tcaactggcg caacttcccg tttcggcact gcaaatgccg ttgcattgtc
     3001 caccgcaagc cattgcacat catcaacaac tcgaacagca ggcactggca tcgctgcaac
     3061 actggggagt tttccatgtc tgaactgaac gatcttctga ccacccgtga gctacaacgc
     3121 tggcgattaa ttcttggcga agcggcagaa acgacgcttt gtgggctgga tgacaacgcc
     3181 cggcagatag atcacgcgct ggagtggctg tatgggcgcg atcctgaacg gctccagcgt
     3241 ggtgaacgct ctagtggatt aggtggctca aatctcacca ctcctgagtg gatcaacagt
     3301 attcacacgc tgtttccgca acaggtgatt gagcggctgg aaagcgatgc cgtactgcgc
     3361 tacggcattg aagatgtggt gacaaatctc gacgtgctgg aacgtatgca gccttccgaa
     3421 agcctgctac gcgccgtttt gcacaccaaa catctgatga accccgaagt actggctgcc
     3481 gcccgcagga ttgtgcgcca ggttgtggaa gaaattatgg cgcgactggc aaaggaagtt
     3541 cgtcaggctt tttctggtgt ccgcgatcgt cgtcgccgct catttattcc actggcgcga
     3601 aactttgatt tcaaaagtac tctgcgcgcc aacctgcaac actggcaccc gcaacacggc
     3661 aagttgtata tcgaattccc ccgctttaac agccgcatta aacgccaaag cgagcaatgg
     3721 caactggtct tactggttga tcaaagcgga tcgatggtcg attcggtgat ccactctgcg
     3781 gtgatggcgg cctgtttatg gcagttaccc ggcattcgta cccatctggt ggcgtttgac
     3841 acaagcatcg ttgatctcac ggcagacgtt gccgatccgg tagagttatt aatgaaagtg
     3901 caattaggcg gcggaaccaa tatcgccagt gccatggagt atggtcggca acttattgaa
     3961 caacctgcaa aaagcgtcat tatcctcgtg agcgattttt atgaaggggg ttcatcatca
     4021 ttgctgacgc accaagtgaa aaagtgtgtc cagagcggca tcaaagtgct gggactggca
     4081 gcgctcgata gcacagcaac accttgctat gaccgcgata cggcccaggc gctggttaat
     4141 gtcggcgcac aaatagccgc catgacaccg ggcgagctgg catcatggct tgcggagaat
     4201 cttcagtcat gaattcacta cgtccggaat tattagaact gacaccgcag gcactgacgg
     4261 cgttaagcaa tgccggtttt gttaagcgca gccttaagga actggaaaat ggcaacgtcc
     4321 cggaaatcag ccatgagaac agcgctttaa tcgccacctt cagtgacggt gtccgtaccc
     4381 agctggcgaa cggccaggca ctgaaagagg ctcagtgcac ctgtggggcc agcggcatgt
     4441 gccgtcatcg cgtgatgctg gtgttaagtt atcaacgact ttgtgctacc gcccagccta
     4501 cgggaaaaga agaggaatgg gatccggcaa tctggctgga agaactggct acccttcccg
     4561 atgccacccg caaacgcgca caggcgctgg tcgctaaaag catcaccatt gagttgttct
     4621 gtgcgccggg tgaaattccc tctgcccgct taccgatgag cgatgtgcgt ttttattccc
     4681 gcagcagtat tcgtttcgcc cgttgtgatt gtattgaagg cacactttgc gaacatgtcg
     4741 tactggcagt acaggccttt gtccaggcaa aagcgcagca agcggaattg actcatttaa
     4801 tctggcagat gcgcagcgaa cacgtcacat catctgacga tccgtttgcc agcgaagaag
     4861 gcaaaacgtg tcgtcaatat gttcaacaac taagccaggc attatggctg agcggcatca
     4921 gccagccgct tattcactac gaggccgctt tcagccgcgc gcagcaagca gcggaacgct
     4981 gcagctggcg atgggtgagt gaatcgctac ggcaactgcg cgccagcgtt gatgccttcc
     5041 acgcccgcgc cagccactat catgccggag aatgcttacg tcagcttgcg gcattaaaca
     5101 gtcgattaaa ttgcgcacaa gagatggccc ggagcgacag tgttggtgaa gttcctcctg
     5161 tgccgtggcg cacggtcatt ggctctggca ttgccggaga agcaaagctt gatcatctgc
     5221 ggctggtgtc tttaggtatg cgttgctggc aggatattga gcattatggt ttacgcatct
     5281 ggtttaccga tcccgacacc ggcagtattt tgcacctttc gcgcagttgg ccgcgaagtg
     5341 aacaggaaga ctcaccggca gctacgcgtc ggctgtttag ttttcaggct ggcgcactgg
     5401 cgggtgggca aattgtttca caagcagcaa aacgcagtgc cgatggcgag ctgctgttag
     5461 ctacccgcaa ccgcttaagc agcgttgtgc cgctgtcgcc tgatgcctga tgcctggcaa
     5521 atgttgagcg cgccgttacg ccagccgggc attgtggctt tgcgggaata tttacgccag
     5581 cgtccccccg cctgcatacg gcctcttaat caggtcgata acttatttat tctgccggtc
     5641 gctgagtgta tttcgctcgg ttgggatagc agccgccaga cgctggatgc gcaggtaatc
     5701 agcggcgaag gtgaagataa tctgctgacg ttatcactgc cagcctcagc cagcgcgcct
     5761 tatgccgttg aacgcatggc ggcgcttttg caacaaacag acgaccccgt gtgtctggtt
     5821 tctggctttg tcagttttgt tgatgggcaa ttgacactgg aaccacaggt aatgataaca
     5881 aagactcgtg cctgggcgct ggacgcagaa actgcgcctg tggtggtatc actaccttct
     5941 gccagcgttc tgcctgtgcc gtctactgct catcagttgc tgatgcgctg ccaggcgtta
     6001 cttattcaac tgctccataa cggctggcgc tatcaggaac agagcgctat tagtcaggca
     6061 gagttgctgg cgaatgacct caccgcggtg ggtttttatc ggctggcaca tgtgttggca
     6121 caatttcgta atacagaaag cgaggcacgg gtagaagcaa tgaataacgg tgttttgctt
     6181 tgcccccatg cttcagcaac aaggatgaaa tagtgctttt tactaagagt tctactccag
     6241 ttccggactg ctcacgccac ggtattaggc atatcctata tagcccctgg tgagagtcac
     6301 cagtttcttg attaaataaa atggagtttt acatgaaggc tttcaataag ctgttttccc
     6361 tcgttgttgc atctgttctg gttttctctc ttgctggctg cggtgacaaa gaagaatcga
     6421 agaaattcag cgccaatctg aacggcactg aaattgccat tacctatgtc tacaaaggtg
     6481 acaaggtgct taagcaatct tctgaaacca aaattcaatt tgcctccatt ggtgcaacca
     6541 ccaaagaaga tgctgccaag acacttgagc cgttaagcgc caaatacaaa aacatcgcgg
     6601 gtgttgaaga aaaatcaacc tataccgata cctacgcgca ggaaaacgtg actatcgata
     6661 tggaaaaagt ggattttaaa gccctgcagg gtatttcagg aatcaacgtt tctgctgaag
     6721 atgccaaaaa aggtatcact atggcgcaaa tggaactggt gatgaaagcc gctggtttta
     6781 aagaagtgaa ataatctgtc ggcggccatg tttcgcatgg ccgccatacc cgtcttagct
     6841 tttcttcaca tgctggcgcg ctgccagtcc acgcagaaaa taacgtaaaa attgatcgcc
     6901 gcattcgcgg aagtttttat gatccggtgc gcgcatcatc gctgtaattt ccggcatcga
     6961 aacgcggaac tgctgttcgg tgaggatagc cagaatgtca tcggttttca gcgaaaacgc
     7021 gatgcgtaat tttttcagca cgatgttgtt attaatgcga cgttccggct ccagtgccgg
     7081 agcagactca tccttgccgc gtttttcata aatcaggcca ttgaggaatg acgacaaaac
     7141 aatgtccgga caacgctgaa aaccctcttc gtcttcttta cgtagccaga cggcgatctg
     7201 ttccgcggtg gcttcgacat tatccagcgc cagaatacgc accaggtcat tattattggc
     7261 tttcaaaatg tagcgcacgc tgcgcagaat atcgttactt agcatgaggc cttcaggtgt
     7321 tgatgaggca aaaagccatt ttagcagtct tttacaggcc aatcgcctct tttaagcttt
     7381 tcagataacg gcggctgacc ggcacggtta aaccattacg caaaatcaac tcggcctggc
     7441 cgttatcttc caaacgaatc tcctgtaaat gcgcgaggtt aaccagatac tgacgatggc
     7501 agcgcagtag tggtgtacga ctttccaggg tacgtaatgt caattcggta aagccctctt
     7561 tcccttcgtg gctggtaacg tagacaccgc tcatccgact gctgacaaat gccacatctt
     7621 tcatttgcag caaataaatc cgactatgcc ccgtacaagg gataaatttc agcgcctgtt
     7681 gattttccgg taacagcgaa acatcctgct tgctgcgctc ctgacgcaat cgcgccagcg
     7741 ttttctccag tcgcgcttca tcaattggct tcagcagata atcaaaggca tgttcttcaa
     7801 aggctttaat agcgtattcg tcaaacgcag tgagaaaaac aatatacggg cgatgttccg
     7861 ggtcaagcat ccccaccatt tccagaccac tgatgcgcgg catctggata tcgagaaaca
     7921 gcacatccgg gcgcagttta tgcaccgcgc cgatcccttc cacggcgttt gaacactctc
     7981 caacgatttc aatatcgctc tgctcctgca aaaatataca caggttctcc cgtgctaacg
     8041 gttcatcatc aacaattaag actttaatca tgcctcgtcc ctccatggta gtcgtaacgt
     8101 tattcgggtg taactatcag gctcacaggc gacgcttatt ccatagtcat cgccaaaccg
     8161 ttcacgtaaa cgcttatcca ccagattcat ccccagccca ctggcattgg ttaccggttg
     8221 atacaaaccg gcattgtctt cgatctccag catcaaatgt tgcccctcac gtcgggcgct
     8281 gattgccact cgccctgtat ccagcagttg tgatgtccca tgtttaatgg cgttttccac
     8341 tatcggttgc agggtaaacg cgggcaattg ctgctgggat aattcttgcg gaatagcaat
     8401 gttgacctgc aaccgcgact ggaagcgcgc cttttcaatt tgcagataag cgttcacatg
     8461 ttcaatttcg tcggcgagag taacaaactc cgaaggccgc tttaagtttt tgcggaaaaa
     8521 agtggaaaga tactgcacca gctggctggc ctgttcgctg tcgcggcgga tcaccgcttt
     8581 aatggtgtta agcgcattaa acaaaaaatg aggattcacc tgggcgtgaa gcagtttgat
     8641 ttctgactgg gtgagcatcg ctttttgccg ctcatattgc ccggcaagaa tctgcgccga
     8701 aagcagttgc gcaatcccct cgcccagcgt gcggtttatt gaactgaata aacggttttt
     8761 ggcttcatac aatttgatgg tgcccatcac acgctgattt tcgccacgca acggaattac
     8821 cagcgtcgac cccagtttgc attgcggatg caaagagcaa cgataaggta cttcgttgcc
     8881 atcagcgtag accacttcac cggtttcaat cgcttttaag gtgtaagtcg aagaaatcgg
     8941 tttgccgggt aaatggtggt cgtcaccaat tccggtaaag gccagcaatt tctctcgatc
     9001 ggtaatcgcg actgcaccaa tatccagctc ctgatacagc acctgagcca ctttcatgct
     9061 gttcacttcg ttaaatccct gtcgcaaaat gccttccgtc gaggctgcca ctttcagcgc
     9121 agtggcagaa aaagccgaag tgtatttttc aaacatcgcg cgtttatcga gcaatatacg
     9181 cataaacagc gccgcgccga cggtattggt gaccatcatt ggcgcagcaa tattactcac
     9241 cagacgcacc gcatcttcat aaggtcgggc gatcgcaagg atgatcagca tttgcaccat
     9301 ttcagcgacg aacgtgacgg caccggcggt aatggggtta aagactttat cagtgcgccc
     9361 gcgacggatc aggatgctgt gtaccaggcc gccgagcaat ccttcaacga tggttgagat
     9421 catgcaactt agcgcggtca tgccccccat cgaatatcga tgtaagccgc cggtcagacc
     9481 aaccagccca ccgacgaccg gaccgccgag taagccgccc attaccgcgc ctatcgcacg
     9541 ggtattggca atagaatcgt caatgtgcaa cccaaaccag gtgcccatga tgcagaagat
     9601 ggaaaagacg atgtagcaga gaaatttatg cggcagacga accgtgacct gcattaacgg
     9661 tatgaataat ggcgttttac tcattaacca cgcaatgact aaaaaaacgc acatctgctg
     9721 aagcagcagc aacaccagat taaaatcgta catacccgca aaccacactt ccctttaaaa
     9781 cgcgtaacat acattgcctg cgtttaactt tctttgaact cttgcagaaa aatgagaatt
     9841 cgtgagtacg atcactcaaa atcgcctggc aaaaataaaa tcaccctata gatgcacaaa
     9901 aaacgggcaa aactacctgg ttcgcaaaac tgcgtctaaa gttaaactgg gacctcgcga
     9961 gcaagggtga gatgatggcg ctttacacaa ttggtgaagt ggcgttgctt tgtgatatta
    10021 atcctgtcac gttacgcgcg tgacgcaaca tttttaaaaa tcgacgaaga tcgtccagga
    10081 gcgccgtttt tcaagggttg gatagatgat tcgtttacca tgtcgacgaa cttcgacgaa
    10141 tttcgccgat aaaaagtgtc ccatacatgc cccattgatg ccccataaac aatatcagga
    10201 attgacactg gttatccata cagataaaaa taacacctgt acataactac agttacagga
    10261 gggtgttatg cgtgttgaga tctgtatcgc taaagaaaaa atcactaaaa tgccaaacgg
    10321 tgctgtggat gcgttaaagg aagaattaac ccgacgcatc agtaaacgtt atgacgatgt
    10381 agaggtgatc gtaaaagcca ccagcaacga tggcctttct gttacacgca ccgcagataa
    10441 ggattctgca aaaacatttg ttcaggagac tctgaaagat acctgggagt ctgctgacga
    10501 gtggtttgtt cactaataaa cacgtaaaat cggtaacggc tggaaatcat tcaatactcg
    10561 cactatcgaa agtttaccag ccaaccgcag cacgtcctgc atacgtcgtg tctgcggttt
    10621 ttctttttcg cttacattgt gtctggttct tccggccact caatatcagg tgcagttgat
    10681 gtatcaacac ggttcagcaa cacccgatac ttcttccagg cttccagcaa cgagttttct
    10741 tcctccgttg cgatctccag atctacagca tcctgaagtg gcgcaatatg ctcactgaat
    10801 ccctggatgt agaactgtgt ggtgacggtc ttccagccat tcggctcctg ctgtatcgaa
    10861 gcataccagg ctatttcaat atcgctatgc tgcggcagca tttaacccct tgtaattcat
    10921 cgccataatt gatttaattc acaaataaaa ctataatatg gtgaaattaa tgaaaaaaaa
    10981 cacagatgat ggggctaaaa tttacacacc acttacccta aagctttatg actggtgggt
    11041 tttgggagta tcaaatcggc ttgcatgggg atgtcctaca aaggaacacc ttcttccaca
    11101 ctttctggaa catttaggta acaaccatct ggatattggt gttggaactg ggttttacct
    11161 tactcacgta cctgagagta gtctgatatc tttaatggat ttgaacgaag ctagcctgaa
    11221 cgcggcatct acaagggctg gggaatcaaa aattaaacat aaaattagcc atgatgtttt
    11281 tgaaccttat cctgcggcgt tacatggtca atttgattcc atttccatgt tttaccttct
    11341 tcactgcctg cctggaaata tatctacaaa aagctgtgta atacgcaatg cggcgcaggc
    11401 cttaactgat gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa
    11461 tagcttcggt caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa
    11521 agattccgaa gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccaa
    11581 ggttcaaggt actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc
    11641 acgttcttgc ttaagacgtg ctacggtttc ctttacgatt tggggttgcg actttacctc
    11701 tattgataat gcattccggc agaacgttca aatatgaacg ttcgatattt aactaaccga
    11761 aaaacaaaat atagcacagg cgagacattt accatctaag aaaatagtat cgttttttac
    11821 agttaaatca acatcacttc cttaaaatga aaacaataaa tttcaatcct gaaacatttt
    11881 atttcagttt attatttccc agtatcacat tttatcagaa tatccttctg cgaggttatt
    11941 atgcttcctg taaataatcc ccccctatcc actggaaacg tctcttttta cagaactaca
    12001 tcaatcgaca atgttcacaa taattatctc tccgaatggg ttgaatggac taaaaacagc
    12061 atttccggag aaaacaggga aactgctttt acccggctcc aattatgtct ggagaacagt
    12121 gaaacatcgt tggacttatc ttgtttaggt ctcagatctc taccacgatt gcctgacaat
    12181 cttgatgaaa ttaatgtaag caataaccaa ctatcaatgc tccccgagct accaagggca
    12241 ttgaaagagc tgaatgcaag cagtaatcaa ttatctgcac ttcctgaatt acctgcgtca
    12301 ctagaatata ttaatgtaag tgacaatcac ctgtctgtac ttccgaggtt accaatgtca
    12361 ttggaattac ttgatgcagc cagaaatgct ttggaagtaa taccagattt tccagaaaga
    12421 gatgatcata ttataagaat attctggctt aatcagaacc ggatcacggc aattccggaa
    12481 agcatacttg gcctcagttc tgatagcgtt gtcaatctta gagaaaatca actatctccc
    12541 agaataatgc aaactttgtt
//
LOCUS       DBKEKI010000117        12475 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.117, whole genome shotgun sequence.
ACCESSION   DBKEKI010000117 DBKEKI010000000
VERSION     DBKEKI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.117"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..161
                     /locus_tag="ACUQPX_003200"
                     /pseudo
     CDS             1..161
                     /locus_tag="ACUQPX_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            159..707
                     /gene="tnpA"
                     /locus_tag="ACUQPX_003201"
     CDS             159..707
                     /gene="tnpA"
                     /locus_tag="ACUQPX_003201"
                     /inference="COORDINATES: protein motif:HMM:NF038385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HHV9057812.1"
                     /translation="MAGRRLGVPKSTVCSMFVRFRKAGLSWPLPAGMSERELDARLYG
                     SASTVPVVLTESTVMPEVPGVKKRPRRPNFPYEFKIALVEQSLQPGACVAQIARENGI
                     NDNLLFNWRHQYRKGGLLPSGKNMPALLPVTLTPEPDNHGFDIIYMLSTHHQRFTFVR
                     LFDPYLIGSRPTFSHLAHHHIS"
     gene            complement(801..1157)
                     /locus_tag="ACUQPX_003202"
     CDS             complement(801..1157)
                     /locus_tag="ACUQPX_003202"
                     /inference="COORDINATES: protein motif:HMM:NF012307.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HHV9057813.1"
                     /translation="MIQAQEYIGAGYHWVVDLDLEKFFDRINHDVLMSRIEKRVSDKR
                     VLSLIRRFLNAGVMDAGLVRPVTEGTPQGGVISPLLSNLFLHYAFDMWMQRQCPDVPF
                     ERYADDGVPRRRAPGT"
     gene            1119..1499
                     /locus_tag="ACUQPX_003203"
     CDS             1119..1499
                     /locus_tag="ACUQPX_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057814.1"
                     /translation="MIPGPDIFLGLYHCLVGRTTGTEPVAVVAERAISQCLQYLHHSL
                     LDEAIHHHLDAQQTFAAAGLQYGYSSHRGWAVSAGQQLRFQLWPVVPQVVRQFTYAHA
                     IDSRRTLIASCRWNSNQRARGRQR"
     gene            1447..2229
                     /locus_tag="ACUQPX_003204"
                     /pseudo
     CDS             1447..2229
                     /locus_tag="ACUQPX_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS421 family transposase"
     gene            2807..3232
                     /locus_tag="ACUQPX_003205"
     CDS             2807..3232
                     /locus_tag="ACUQPX_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HHV9057815.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIVIHPECNAIYAALD"
     gene            3279..3701
                     /locus_tag="ACUQPX_003206"
     CDS             3279..3701
                     /locus_tag="ACUQPX_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HHV9057816.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSEFVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMEVVLQALDGEHMS"
     gene            3698..3889
                     /locus_tag="ACUQPX_003207"
     CDS             3698..3889
                     /locus_tag="ACUQPX_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057817.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            complement(4259..5932)
                     /gene="ltrA"
                     /locus_tag="ACUQPX_003208"
     CDS             complement(4259..5932)
                     /gene="ltrA"
                     /locus_tag="ACUQPX_003208"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HHV9057818.1"
                     /translation="MNTLIKVSAAPHLGSWHTINWKRQYRTVRRLQTRIVKATQEKQW
                     RRVKALQRLLTRSFAAKALAVRRVTENSGRKTPGIDGKTWSTPAEKWNAINNLRRNGY
                     KPSPLKRIYIPKSNGKLRPLGIPTMKDRAMQALYLLALEPVAETTADRNSYGFRPHRS
                     CADAIEQVFNNLNRKTSATWVLEGDIKGCFDNISHKWLLEHIPMDKRILRSWLNAGFM
                     EKGRLFTTDAGTPQGAPCSPVLANMCLDGLETILTNRFGRKGTAKASKSKVNYVRYAD
                     DFIVTGISREILESEVKPLITAFMQERGLLLSEEKTTVTHINDGFDFLGQNVRKYKGK
                     LLIKPSKHNLKMFLNKIRATIEGNKTAKTDTLIKRLNPLIRGWTNYHQHVVAAETFRY
                     VNFQLWKKIWRWCLRRHPSRGKRWIRRKYFDESWRLHSKDADGHRWVLYKASTIPIRR
                     HIKIKSEANPYAPEWESYFEKRQALIWLKSQNGRNRIVAIWRKQEKRCPICGQSFSQE
                     TGWDIHHIVRKTQGGSDNLDNLVMLHPNCHRQLHHSSETGSQYWGLIKA"
     gene            complement(6536..6725)
                     /locus_tag="ACUQPX_003209"
                     /pseudo
     CDS             complement(6536..6725)
                     /locus_tag="ACUQPX_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6795..6953
                     /locus_tag="ACUQPX_003210"
     CDS             6795..6953
                     /locus_tag="ACUQPX_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HHV9057819.1"
                     /translation="MYIPGQLFPAVAVMPLAAKVLKKVMVNTGGNKRQVPAKRQNRGS
                     RNSTDDGG"
     gene            6978..7265
                     /locus_tag="ACUQPX_003211"
     CDS             6978..7265
                     /locus_tag="ACUQPX_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057820.1"
                     /translation="MSTRNIHVNTASYTLFVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            7334..8155
                     /locus_tag="ACUQPX_003212"
     CDS             7334..8155
                     /locus_tag="ACUQPX_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HHV9057821.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMEVMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSWRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(8451..9098)
                     /locus_tag="ACUQPX_003213"
     CDS             complement(8451..9098)
                     /locus_tag="ACUQPX_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HHV9057822.1"
                     /translation="MIQIANQQKLVFAWGVVLLVVRTTNLLSLFVEWVKLFTDKVTRG
                     GKMKKWMFAICLMFINEICHATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINP
                     VTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTSAYYLAIAFKKWGVSWEAV
                     GAYNAGFRKTERQNQRRLAYASDVYRIYTGIKSSKGIRIPTTKKSLPEINSVQNN"
     gene            9384..9767
                     /gene="traM"
                     /locus_tag="ACUQPX_003214"
     CDS             9384..9767
                     /gene="traM"
                     /locus_tag="ACUQPX_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HHV9057823.1"
                     /translation="MAKVQAYVSDEIVYKINEIVERRRAEGAKSTDVSFSSISTMLLE
                     LGLRVYEAQMERKESAFNQAEFNKVLLECAVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ANIVEDIREKVSPEMERFFPKNDDE"
     gene            9961..10647
                     /locus_tag="ACUQPX_003215"
     CDS             9961..10647
                     /locus_tag="ACUQPX_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HHV9057824.1"
                     /translation="MCALDPKERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKNRKIL
                     YANGAFIELFSREDKPLSGESYIRLQVEIFLSSLELECQALGHGSAFCRRFNFHGEIY
                     QIRMENISFYNDESVVLWQINPFPDYPFFALNQSGSNTNTSDKLTIWNDLSPGTLVVF
                     SFYMLGVGHATIARELGITDRASEDRIRPVKRKIKEFFEHFDLFRVSCIYKGEIDSLL
                     SIIREFYSVK"
     gene            10741..10968
                     /gene="traY"
                     /locus_tag="ACUQPX_003216"
     CDS             10741..10968
                     /gene="traY"
                     /locus_tag="ACUQPX_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HHV9057825.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVTEESESTFKEL"
     gene            11002..11367
                     /gene="traA"
                     /locus_tag="ACUQPX_003217"
     CDS             11002..11367
                     /gene="traA"
                     /locus_tag="ACUQPX_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HHV9057826.1"
                     /translation="MNAILSVQGASAPVKKKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMASAGKGTDLMAKGQETVKATFGKDSSIVTWVILAEVLVGAVMYMMTKNVKFL
                     AGFAILSVFIAVGMAVVGL"
     gene            11382..11693
                     /gene="traL"
                     /locus_tag="ACUQPX_003218"
     CDS             11382..11693
                     /gene="traL"
                     /locus_tag="ACUQPX_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HHV9057827.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            11715..12281
                     /gene="traE"
                     /locus_tag="ACUQPX_003219"
     CDS             11715..12281
                     /gene="traE"
                     /locus_tag="ACUQPX_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HHV9057828.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSVDASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            12268..>12475
                     /locus_tag="ACUQPX_003220"
     CDS             12268..>12475
                     /locus_tag="ACUQPX_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK domain-containing protein"
                     /protein_id="HHV9057829.1"
                     /translation="MRKNNTAVIFGGLFFSCSVMAGAGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALT"
BASE COUNT         3003 a         2971 c         3115 g         3386 t
ORIGIN      
        1 acggcacgga accacggcag cttgacggct gcaatgacgt gtgtcgtctg gcggtggaga
       61 atctgctgta tgcagcccga aaacgggggc tggaaatcgg catcttctgc accatccaca
      121 ctttacgcct tcactttgaa gaacatttac ccctcgttgt ggccggacgt cgcctgggcg
      181 taccaaaatc aacagtttgt agtatgtttg tgcgcttccg caaagctggc ctttcatggc
      241 ctctgcccgc aggtatgtcg gagcgggaac ttgacgcccg tctttacggg agtgcctcca
      301 cagtacctgt cgtacttact gaaagtaccg ttatgcccga agtccccggg gtaaaaaagc
      361 gtccccggcg ccccaacttc ccttatgagt tcaaaatcgc cttagtagag cagtcactgc
      421 agcccggagc ctgtgtggcg cagattgccc gtgaaaatgg aatcaacgat aacctgctct
      481 tcaactggcg ccatcaatac cggaaaggtg gcctgctgcc ttccggaaaa aatatgccgg
      541 cactgcttcc cgtgacgtta acgccggagc cggataacca tggttttgac atcatctata
      601 tgctttcgac acatcatcag cggttcactt tcgttcgtct cttcgatcca tacctgatcg
      661 gctcacgtcc gaccttttcc catctcgctc accaccacat ctcttaaatg cagcagcagt
      721 gggtggtttg gtgcctgcgc ctgcacgccg acaccgaggg gccttccctc atctcttgtg
      781 cagcatggct gcacagggac tcacgtccct ggtgcgcgcc ttcgtggcac accatcatcg
      841 gcgtaccttt cgaagggaac gtcgggacac tgacgttgca tccacatatc aaaggcatag
      901 tgcaggaaca gatttgagag caggggcgag atcacgccgc cctgcggcgt tccttctgtc
      961 accggcctta ccagacctgc gtccatcacc cctgcattca ggaacctgcg gataagtgac
     1021 aacacccgtt tatccgatac tcgtttctct atccggctca tcagcacgtc gtgatttatc
     1081 cggtcgaaga acttctccag atcgaggtcg accacccagt gatacccggc cccgatatat
     1141 tcctgggcct gtatcactgc ctggtgggcc gaacgaccgg gacggaaccc gtagctgttg
     1201 tcgctgaacg agctatctca cagtgcctgc aatacctgca tcatagcctg ctggatgaag
     1261 cgatccacca ccatctggat gcccagcaga cgttcgccgc cgccgggctt cagtatggat
     1321 actcttctca caggggatgg gcggtatctg ccggacagca gttgcgcttt cagctctggc
     1381 cagtggtgcc tcaggtagtc cggcagttca cttacgcgca tgccatcgac tcccgccgca
     1441 ccttgattgc gtcttgtcga tggaacagca atcagcgcgc ccgggggcgg cagcgctgaa
     1501 tggcgactac atatgggata tgatcctcat acctgtcagt tcactgattt tgagctaacc
     1561 gacagcagag acgctgaacg gctggatcga tttgcgcaaa cggcagacga gatacgaatt
     1621 gctgaccggg gattcggttc gcgtcccgaa tgtatccgct cacttgcttt tggagaagct
     1681 gattatatcg tccgggttta ctggcgagga ttgcgctggt taactgcaga aggaatgcgt
     1741 tttgacatga tggattttct gcgcgggctg gattgcggta agaacggtga aaccactgta
     1801 atgataggca attcaggtaa taaaaaagcc ggagctccct ttcctgcacg tctcattgcc
     1861 gtatcacttc ctcccgaaaa agcattaatc agtaaaaccc gactgctcag cgagaatcgt
     1921 cgaaaaggac gagtagttca ggcggaaacg ctggaagcag cgggccatgt gctattgcta
     1981 acatcattat cggaagatga atattcagca gagcaagtgg ctgattgtta ccgtctgcga
     2041 tggcaaattg aactggcttt taagcggctc aaaagtttgc tgcacttgga tgctttgcgt
     2101 gcaaaggaac ctgaactcgc gaaagcgtgg atatttgcta atctactcgc cgcattttta
     2161 attgacgaca taatccagcc atcgctggat tttcccccca gaagtgccgg atccgaaaag
     2221 aagaactaac tcgttgtgga gaataacaaa aatggtcatc tggagcttac aggtggccat
     2281 tcgtgggaca gtatccctga cagcctacaa aacgcaattg aagaacgcga ggcatcgtct
     2341 taacgaggca ccgaggcgtc gcgttcttca gatggttcaa cccttaagtt agcgcttatg
     2401 gggtaaatcc ccgccttttt cctggcggtc cccggacaga gcacaaagga agtcgccgcc
     2461 ggctccgctt tacccggcca tgcggtgcat ggccttgtgg gttttcagct ctgtagcctc
     2521 agcgtcgtgt gcgggctgtg ccgtgcctcc atcttagccg ggctggcggg gatgcaaggg
     2581 tacgcttcgc cgctgcggtc acccggtccc tccttcccgt ctgccgtgat tttccggtcg
     2641 ttcacccgct caggtttcgc ggtctcaccc tccaactccc gccagccgtg tgcgtcaggc
     2701 tgcggcttcc cttgcgccct gtgcatcccc gccttatcgc ccggctaaga tggaggcacg
     2761 gcaccgcccg gtgtcgcgga atatgtgaac aacggaggaa aacatcatgc aatatgcgaa
     2821 acctgtcact ctgaatgttg aagagtgcga ccgtctctcc tttctgcctt acctgtttgg
     2881 caacgatttt ctgtatgccg aggcgtatgt gtacgcgctg gcgaaaaaaa tgatgccgga
     2941 atatgaaggc ggcttctggc acttcatccg cctgccggac ggtggcggtt acatgatgcc
     3001 ggatggcgac cgtttccaca tggtgaacgg tgcaaactgg tttgaccgta ccgtgagtgc
     3061 tgatgccgca ggcatcattc tgacttctct tgtgattaac cgccagttgt ggctgtacca
     3121 cgacagcggg gatgcaggac tgacccagct ttaccggatg cgcgatgcgc agttgtggcg
     3181 tcacatcgta attcaccctg aatgcaacgc gatttacgcg gcactggact gattaacgga
     3241 cggggcggca atgccgcccc tgtaaaggag acgagaacat gtactgtact gttaaagaaa
     3301 ttatccgcga tgtactggat acagacgtgc cggacagtga atttgttttt gccgtggtgc
     3361 tgacccgtgg ggatgtgcgt cacatagccc aggactggag tctgacagac gatgagctgg
     3421 aaaccgtcat gcagcggctg gacgatgctt ttgtatatgg cgcatgtgac agagttgttt
     3481 ccgatattgt caatgaactg atggaggaaa agcgcgtaaa ccgtcttgta acagtccctg
     3541 cggtgatgct ggaaaaagtg atggcgctgg caggcagtga aatgaagcgc ctgtatgccg
     3601 tcgggagtga gaacgggggc gacggtgacg cattcgtcag ggaggaacgc gaagcaatgg
     3661 aggttgtgtt acaggcgctg gacggggagc acatgtcatg aatatcagca cagaaacccg
     3721 cgaaattctg cgtaattaca gggccgtgat taatgcgcgg cgtcgtgaaa tggggcagaa
     3781 accgctcacc accgcgcaga ttgttgatga aatctgcgat tttgtggcga atcagcaggc
     3841 ggttttcctc ggtggtcact acatccttca gggcagcagg cacaggtgat acagggcagg
     3901 cggtggaggc cgcctgtcag cccgcgcgaa aacccctggc aggctgacgc ctgccggaag
     3961 ggaagcgggc cacgctgacg cgtcgcccgt tacccttccc gtttatgatg tgtccggaat
     4021 atttttttct gatgttattt ttctgtggtt accgtgcctg ttattgttct tcttcctctt
     4081 cttctttccg ttcctctcct ctcctgttta tggctttccc gggcgtcccc ttggcggggc
     4141 tggctgtcgt gaacggagcc agcgaagctg tctccgccct ggcgggtcgg gtagcagcgg
     4201 cacattgctg cgccgccgcc ccctaagaac ggagcgtgcg agtttccccg cactccgctc
     4261 aagcctttat aagcccccaa tactgagagc cggtttcact actgtggtgg agttgacggt
     4321 ggcagttcgg atgcagcatc actagattat ccagattgtc gctcccccct tgtgtttttc
     4381 gcacaatgtg atggatatcc caacccgttt cctggctgaa cgattgtcca catattgggc
     4441 agcgtttttc ctgttttctc catattgcta caatcctgtt tctgccattt tgagatttca
     4501 gccatatcaa tgcctgtctt ttctcaaaat aagactccca ctcgggggcg tatggatttg
     4561 cttcggattt tattttgatg tggcgtcgta taggtattgt cgatgcttta tacagtaccc
     4621 aacgatgacc gtcggcgtcc ttcgagtgaa gcctccagct ttcgtcgaag tatttgcggc
     4681 gtatccatcg tttacctctg gaaggatgtc tcctgagaca ccatcgccat atctttttcc
     4741 agagttggaa gttgacgtac ctgaaggttt cggctgccac tacatgttgg tggtagttgg
     4801 tccaacctct gatcagaggg tttagccgtt tgatcagtgt gtctgttttg gcagttttat
     4861 taccttcgat ggtggctcta atcttattaa ggaacatttt caggttatgt ttagagggtt
     4921 ttataagtaa cttccccttg tatttcctga cattttgtcc aagaaagtca aagccgtcat
     4981 ttatgtgagt aaccgtcgtt ttttcttctg acaggagcaa ccctctttcc tgcatgaatg
     5041 cagttataag cggttttact tcgctttcca gtatttctcg cgaaattcct gtcacaatga
     5101 aatcatcggc gtaacgcaca tagttcactt tggatttact ggccttggct gtgcctttcc
     5161 gcccgaaacg gtttgtcaga attgtttcca gtccgtccag gcacatattt gccagtactg
     5221 gtgagcaggg ggcaccctga ggtgtaccag cgtcagtagt gaacaatctt cctttttcca
     5281 taaatccggc attcagccag ctacggagga tccgtttatc catagggata tgctccagta
     5341 accatttatg actgatattg tcgaaacacc ctttaatatc cccttccaga acccacgttg
     5401 ccgatgtttt ccggttcaga ttgttgaaaa cctgttcgat tgcatcagca caacttctgt
     5461 ggggacgaaa gccgtagctg ttacgatccg cagtcgtttc cgcgacaggt tccagagcga
     5521 gcaggtagag cgcttgcata gctctgtctt tcatcgtggg gatacccagc gggcggagtt
     5581 tgccattgga tttgggaata tatattcgct tcagaggact cggtttatag ccgtttctcc
     5641 tcaggttgtt gatagcattc catttctctg ccggcgtgct ccaagttttt ccgtcgattc
     5701 ccggggtttt acgcccggag ttctcagtaa cccgtctgac agccagtgct ttggctgcaa
     5761 acgagcgggt cagcagacgt tgcagagctt tgactctacg ccattgcttc tcctgtgttg
     5821 ccttcacaat ccgcgtctgg agccgtctca ccgttcggta ctgtcgcttc cagttgatgg
     5881 tgtgccagct accgaggtgc ggggccgcag acaccttaat tagcgtgttc atcttgcctg
     5941 cctcctgttt cagactgctt tccggcgttt catgtgatta aagaccagat ggaagtctgc
     6001 acgctttcgc gttagggcaa attttgaacc cctataccat ccattacaga tggccatttg
     6061 cgttttccat cctcctctac ccgcattttc aacagcgttc cttacggttc gcttgccctg
     6121 agggcaaaag tacgggctta cctgttttca cttcagatac agatcgggtt aggttccagc
     6181 tctcgaccag aggctttagt gtccgggtat cctcaggaag cataggataa ccagcctaac
     6241 actttttagt tcaggtctat cagcatcttt gacctgttac agggttatga tctttatcac
     6301 tggttcacgt gtgttaacca taccgatcag ccttgcctcc tgtccgcatg atgcttgcag
     6361 acgatcgtct cacctcacgg ctcgacttac ccttcctgag ggagagtacg ttgtcatcag
     6421 agcttatgac cttgccgtta ccagcaacgc cattctgatt aggctaccgc taccggaata
     6481 gcgggatttg tcatatgtct acgcaaatca atatctgaag cgactttcag tcgcacgctt
     6541 ccatccctga cgcgaaaccc cgggcttgca gcgaccgttc cggtcgcgcc gcctctgtcg
     6601 cgccgtggcc tgcgctcccg gcatcccggc aaaaggccgc cgggatgtaa ggcgcaacgc
     6661 cgtttcgcaa accgcgcttc acgtcgttgc ggcgggtccc tgcccgttgt tgtgtccggt
     6721 gccattcacc tgtttaagcc cctgtcgccg ggggcaaaga cagccacaac cgtctccgcc
     6781 agtccagggg ggaaatgtac atccccggac agttgtttcc ggccgtggca gtgatgcccc
     6841 ttgcggcgaa ggttctcaaa aaggtgatgg taaataccgg agggaacaaa aggcaggtcc
     6901 ccgcaaaacg tcaaaaccgc ggctcccgaa actcaaccga tgatgggggc tgaaaaccag
     6961 aaagaggaga aagcaggatg agtacccgaa atatccacgt taacaccgcg tcgtacacgc
     7021 tttttgttgc cgggaagaaa aaaaacaccg gggaagagtg ggacgtcctg gaattcagca
     7081 gcctgactga gctgaaaaaa tatcgcaaaa gccacccgga aaagatggcc ttcagctata
     7141 gctacgcgct cagccggggt gtggatacgc agttccgcca tatcaacatt gcggaagccg
     7201 atcacttcaa acagttcctg cgtcagatta agcgtgccgg cctcgatatc cgggcgatct
     7261 gctgaccgga tgtgatgata ttaagcggaa aaatttttta ttaaatttat gctttcataa
     7321 aggagtagct gttatgcgat tagcttcccg ttttggtcgg tataattcca tccgccgtga
     7381 acgtccttta acggatgatg aattaatgca gttcgtgcct tcggtatttt ccggtgataa
     7441 acatgagtcc cggagtgaac gttatacgta tattccaaca atcaatatca tcaataagtt
     7501 acgtgatgaa ggtttccagc cattctttgc ctgtcagagt cgggttcgtg atttgggacg
     7561 tcgcgaatac agtaaacata tgttacgtct tcgcagggaa gggcatatta acgggcagga
     7621 agttcctgaa attatcctgc ttaattcaca cgatggttca tccagttatc agatgatccc
     7681 cggaattttt cgttttgtct gcacaaatgg cctggtgtgc ggaaataatt ttggcgaaat
     7741 tcgcgttcca cataaaggtg atattgtcgg gcaggttatc gagggagcgt atgaagtgct
     7801 cggtgtcttt gataaggtca ctgaaaatat ggaggtgatg aaagaaattc atcttaacag
     7861 tgacgagcaa catttatttg gcagagctgc actgatggtc aggtatgaag atgaaaataa
     7921 aacgccagtg acgcctgaac aaataattac tccccgtcgt cgggaggata aacagaacga
     7981 tctctggaca acctggcagc gggttcagga gaatatgata aaaggtggat tatcgtggcg
     8041 aagtgcctcc gggaaaaata ccaggacaag agccattaca ggtattgatg gtgatataag
     8101 aatcaacaag gcgttatggg tgattgccga acagttcaga aagtggaagt catgataata
     8161 taagtaaagc cccgaaaaat tttcggggct ttacttatat tatcattctt cggcatcgct
     8221 gtgtcgtctg atatacctca atttcatcaa ctggctttcc ggttgtctgt aaatattgtg
     8281 cccgcgaaaa atggtctggc aggaggctgc gcagttaccg ggtatatata agatgggttc
     8341 ccgcttcgcg gggcttcggc cagtcagtat tgtcactcat atgatatttt tgtgtgtggc
     8401 cttccatgcc gcttttgcgg cataaccggt atctgacaat gtctgtaaga ttaattgttc
     8461 tgcacgctgt taatttccgg aagtgatttc ttcgtggtcg gaatccggat acctttactg
     8521 ctttttatcc cggtataaat ccggtaaaca tctgatgcgt aggcaagacg tctctggttc
     8581 tggcgttcgg tcttcctgaa tccggcattg tatgcaccaa cggcctccca ggagacgccc
     8641 cattttttaa aggctattgc cagataataa gcactggtat aaatgttcat gcagggatct
     8701 gttgtcagat gttccggctt aattccatag cgggccagtt cgttaaaatg ctgggaatct
     8761 acctgcatca gtccgctgcc atatcccgtt accggattaa taccgatggc attaacccgg
     8821 taacgggatt ctttccatga tattgctctc agtaaatcag gatctatttt gtaatcccgg
     8881 cctgcaagat caaagcaatc agtggcatgg cagatctcat ttataaacat caggcagatg
     8941 gcgaacatcc attttttcat ttttccacct ctggtgactt tatccgtaaa taatttaacc
     9001 cactccacaa aaaggctcaa caggttggtg gttctcacca ccaaaagcac cacaccccac
     9061 gcaaaaacaa gtttttgctg atttgctatt tgaatcatta acttatgttt taaataatgt
     9121 attttaattt attttacatt ataaaaagga ttcattggtg aatcgcatat gattcaccaa
     9181 tgaatttaac acaactcata gatgaatcag taataataaa acaacgagtt aaagcgaatt
     9241 gactctagat tcaattcgtg atgtgatttg ctgcatgaat cgtaattttc gtaacttgcg
     9301 tcacgattcg attcatcgat gaattgtcat ttttttatat gtgaaataaa tttcatgcga
     9361 taagaaaata aagaggtata catatggcta aagtgcaggc ttatgtcagt gacgaaattg
     9421 tctataaaat aaatgaaatt gtggaaagaa gacgtgcaga aggagctaaa agtacagatg
     9481 taagtttctc aagcatatcc actatgcttc tcgaattagg gcttcgtgta tatgaggctc
     9541 agatggagcg taaagagtca gcgttcaatc aggctgagtt taataaggta cttcttgaat
     9601 gtgcggtaaa aacacagtca acagttgcta agattttagg tattgagtca ctaagccctc
     9661 atgtttccgg gaatccaaag tttgaatatg cgaatatagt cgaagatatc agggagaagg
     9721 tgtcacctga gatggagagg ttctttccaa aaaatgatga tgaataaaat taaattaaaa
     9781 attgattccc atcaaataac agaatataga actgttctaa tttgatgaca attaaccaca
     9841 taaagagtat attcatcatg tggttaatcc cacattaaaa tttgaaactg aaaatcgccg
     9901 atgcagggag ttatcccctc cctgcatcga ctgtccatag aatccttaac ggaggttcct
     9961 atgtgtgcgc tggatcctaa agaaaggcca cttaacagtc aatctgtaaa taaatacatt
    10021 cttaacgttc agaatatcta taggaattct cctgttccgg tatgtgtccg caacaaaaac
    10081 cggaaaatcc tttatgccaa tggcgcgttt attgagctat tttccagaga ggataaacct
    10141 ttatccggag agagttatat acgcctgcag gttgaaattt ttctttcatc acttgagctg
    10201 gaatgccagg ctcttggaca tggctctgca ttttgtcgtc gttttaattt tcatggcgaa
    10261 atctatcaga taaggatgga gaatatttct ttttataatg acgaatctgt tgttttatgg
    10321 caaattaatc cgtttcctga ttatccattt tttgcgttaa atcagagtgg aagtaataca
    10381 aatacttctg ataaattaac gatatggaat gatctttctc caggaacatt ggttgttttc
    10441 tctttttata tgctgggtgt tggtcacgca acaattgcca gagagttggg tattacagac
    10501 agagcatctg aggatcgaat tagaccagtt aaacggaaaa taaaagaatt ttttgaacac
    10561 tttgatttat tcagagtgtc atgtatctat aaaggagaaa tagattcgct attaagtata
    10621 attcgtgaat tttatagcgt taagtaaatg ttaaataaga cacgccaatt ttggcgtgtt
    10681 ttttttcatc ggtttctgtg caatcagaat tttattagtc gggaaacaga ggtgaattga
    10741 gtgaggaggc gtaacgcgag aggcggtata agcagaacgg tctcggtgta tctcgatgaa
    10801 gatacaaata accgacttat tcgggccaaa gatcgtagtg gcagaagtaa aacgatagag
    10861 gtccagatcc gtctcaggga tcatctgaag cggtttcctg atttttataa tgaagaaatt
    10921 ttcagggagg tgacagaaga gagcgagtca acatttaaag aactttagtt tttattaatc
    10981 catctacaat agaggtaact tatgaatgct attttgagtg ttcagggtgc ttctgcgccc
    11041 gtcaaaaaga agtcgttttt ttctaaattt acacgtctga atatgcttcg cctggttcgt
    11101 gccgtgatcc cggttgctgt tttgatgatg ttgttccctg agttagctat ggcttctgct
    11161 ggtaaaggca cggacctgat ggctaaaggt caggaaacgg ttaaagcaac tttcggtaag
    11221 gactccagta ttgttacatg ggttatcctg gctgaagtgc tggtcggtgc agtcatgtac
    11281 atgatgacca aaaacgtcaa gttcctggcc ggttttgcca tactctctgt atttattgct
    11341 gtgggtatgg ccgtcgttgg cctctgacag gaaataaaac gatgtcggga gacgagaata
    11401 aacttaagaa atatcgtttc ccggaaacac tgaccaacca gagccgctgg tttggcctgc
    11461 cactggatga actgatcccc gcagcaatct gtattggctg gggtatcaca acatcgaaat
    11521 atctgttcgg tattggtgca gcggttctgg tttatttcgg gattaaaaaa ctgaaaaaag
    11581 ggcggggcag ttcctggtta cgtgacctga tttactggta tatgccaaca gccctgctgc
    11641 gcggtatttt tcataatgtt cccgattcgt gtttccggca gtggattaaa tagaactgat
    11701 atcaggattg ttatatggaa cacggtgccc gtttaagtac cagtcgtgta atggccatcg
    11761 cctttatatt tatgtcagtg cttattgttc tcagtctctc tgttaacgtc attcaggggg
    11821 tgaataacta ccgtcttcag aatgagcaac gcactgccgt gacgccaatg gcatttaatg
    11881 ccccctttgc agtgtcgcag aacagtgtcg atgcctctta tttacagcag atggcgctgt
    11941 catttattgc cctccgtctg aatgtttcat cagaaaccgt cgatgcctca catcaggcgc
    12001 ttctgcaata tatccgcccg ggcgcacaga accagatgaa agttattctg gctgaagaag
    12061 cgaagcgtat taaaaacgat aacgtgaact cagccttttt ccagaccagt gttcgtgtct
    12121 ggcctcagta tggccgtgtg gaaattcgtg gtgtgcttaa aacctggata ggtgattcaa
    12181 aacctttcac tgatatcaaa cattacatcc ttattctgaa gcgggaaaac ggggtgacct
    12241 ggctggataa tttcggggaa acagacgatg agaaaaaata atacggcagt aatattcggc
    12301 ggcctgtttt tttcctgcag cgtgatggcc ggagccggta cgctggcccc caccgtggtg
    12361 ccaatggtga acggtggtca ggccagtatt gccatcagca ataccagccc gaatctgttt
    12421 accgttcccg gtgaccggat tatcgccgtg aacagtctgg atggtgctct gacca
//
LOCUS       DBKEKI010000118        12457 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.118, whole genome shotgun sequence.
ACCESSION   DBKEKI010000118 DBKEKI010000000
VERSION     DBKEKI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.118"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1037)
                     /gene="ybaL"
                     /locus_tag="ACUQPX_003221"
     CDS             complement(<1..1037)
                     /gene="ybaL"
                     /locus_tag="ACUQPX_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HHV9057830.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIG"
     gene            complement(1275..2495)
                     /gene="fsr"
                     /locus_tag="ACUQPX_003222"
     CDS             complement(1275..2495)
                     /gene="fsr"
                     /locus_tag="ACUQPX_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HHV9057831.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWMGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            2713..4365
                     /gene="ushA"
                     /locus_tag="ACUQPX_003223"
     CDS             2713..4365
                     /gene="ushA"
                     /locus_tag="ACUQPX_003223"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HHV9057832.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEYGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(4402..4881)
                     /gene="ybaK"
                     /locus_tag="ACUQPX_003224"
     CDS             complement(4402..4881)
                     /gene="ybaK"
                     /locus_tag="ACUQPX_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HHV9057833.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(5085..5879)
                     /locus_tag="ACUQPX_003225"
     CDS             complement(5085..5879)
                     /locus_tag="ACUQPX_003225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HHV9057834.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            6017..6358
                     /locus_tag="ACUQPX_003226"
     CDS             6017..6358
                     /locus_tag="ACUQPX_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HHV9057835.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(6573..9077)
                     /gene="copA"
                     /locus_tag="ACUQPX_003227"
     CDS             complement(6573..9077)
                     /gene="copA"
                     /locus_tag="ACUQPX_003227"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HHV9057836.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITRGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            9339..10271
                     /gene="glsA"
                     /locus_tag="ACUQPX_003228"
     CDS             9339..10271
                     /gene="glsA"
                     /locus_tag="ACUQPX_003228"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HHV9057837.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            10274..11566
                     /locus_tag="ACUQPX_003229"
     CDS             10274..11566
                     /locus_tag="ACUQPX_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HHV9057838.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPCNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            11597..11694
                     /locus_tag="ACUQPX_003230"
                     /pseudo
     CDS             11597..11694
                     /locus_tag="ACUQPX_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11691..12098
                     /gene="cueR"
                     /locus_tag="ACUQPX_003231"
     CDS             11691..12098
                     /gene="cueR"
                     /locus_tag="ACUQPX_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HHV9057839.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(12099..>12457)
                     /locus_tag="ACUQPX_003232"
     CDS             complement(12099..>12457)
                     /locus_tag="ACUQPX_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HHV9057840.1"
                     /translation="SGVAAVITGLVVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRR
                     VREQKHSDSHLNQRGQQLIGRRFVLESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVE
                     VIAIEGITLHIRAVSS"
BASE COUNT         3028 a         3304 c         3313 g         2812 t
ORIGIN      
        1 tcaccaatct gcgccaggct ggcggcgatg gttaatgccg tacgttggga gtgaccaaac
       61 agacggacaa ggaacagggc cgcgaccgat ttaccaaaca gaataattgc cagcgtcgcc
      121 agcactgcca gcggttgctg aatcagaatt aacggatcaa acaacatccc gactgagaca
      181 aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat cgtgggcggc acggtgactc
      241 aattcagact cgttcagtac catcccggca aagaacgcac cgagtgcaaa ggagacatca
      301 aacagctcta ccgcaccaaa ggcaatccct aacgccagcg ccagcactga cagggtaaac
      361 agctcgcgag aaccggttgc cgcgctgcgt gccataatcc acggcaccag acggcgacct
      421 accagcatca taatggcgat aaatgcgatc accttgccga tggtgatccc catatcgact
      481 gcaagagtgg caaagcccac atcgccctgt tccatcattc ctgccactgc gggcagcaac
      541 accagcgtca gaaccatcac caggtcttcc acaatcaacc aaccgatggc gatttgccca
      601 cgctgactgt ctattaattg ccgttcttca agtgcgcgca gtaacaccac ggtactggcg
      661 gtggaaagac ataagccgaa cacgataccg gtcattaacg accagcccag cacggcggag
      721 agcgccatac ccagcagcgt cgccacggct atctgggcga tcgcgccggg aatggcgatg
      781 gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac cgacgccgaa catcagcaaa
      841 ataacgccca gttcagccag ttccggcgca agcttggtat cggcaacaaa gcccggagtg
      901 aatggtcctg ccagcacacc cgctaacaga tatcccacca gaggagaaat acgtagttta
      961 ttggccagca tgccgaggat aaaggcgagc acaaggccgc caacaatggt ggtgataagc
     1021 ggggtggcgt gatgcattcc gtctcctttt cctggtggtt attgtccatt tttggccggg
     1081 aaaaccaaaa ttacaggtaa tagtttatga caatttcatt gatgatgttt atgaataatt
     1141 gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag cacggatcgg aaaacagaag
     1201 gcggtttaac agaggaaagg cttatggcga ctgtaaaacg atgcagccaa agtttgcctt
     1261 tggctgcaat gaaatcagtc tttatgccgg ttatcaggca ggaatatggt caacatcccc
     1321 aatagtggca ggaaagcaca gattttatag actaactcga tgctggtgtg atcggcgata
     1381 agccccagaa ctgccgctcc cagacctccc atgccgaaag caaaaccgaa aaagagtcca
     1441 gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt agaccagaat ggcagagaat
     1501 gccgaagcga ggataaatcc aataatcacc gttaaaaccc ccatccagtg caggcgggcg
     1561 tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag agccccaaat cacatatttc
     1621 cgtccaattt tatcccctac aggcccgccg atcaccgtac ctgccgcaac ggcaaacagg
     1681 aaggcaaaca gatgaagctg ggcattctgg atagataatc cgaatttttg catcagataa
     1741 aaggtgtaat agctgctgat gctcgccata tagaaatatt tcgagaaaat gaggattaac
     1801 agaatgctga ctgccagtac cactttgttg cgcggcagtg gattgattat cgtcgctttg
     1861 ggttttcctt tattcattcg gtgctgtgcc gagtaccaac ggctaatttg cgccaacacc
     1921 acgatcgcca gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc ataaggcgca
     1981 ataatcaccg ccgccagcaa gggtcccagg gaactgccaa agttgccgcc gacctgaaag
     2041 atagattgcg ccaggccatg ccgcccgccg gaagccatac gggctacgcg agaagattcc
     2101 ggatgaaaga ccgatgaacc ggtaccgacc agcgccgccg ccagcagaac tgcgccaaaa
     2161 ctgcccgcca gcgcaagcag caccagacca cttaaggtaa agcacatgcc aattggcaac
     2221 gaccacggca tcggatattt atcggtccag tagccgacca ctggttgcag tagcgaagag
     2281 gcgagctgga aggtgagggt tatcatgcca atctgcataa atgtcagaga aaattctgac
     2341 tgaagcagcg gataaatcgc cagaatcagc gattggatca tgtcgttcag cagatgtgag
     2401 aggctgatag cacctaaaat accaaacgat gttcgggcct tggtcgttga cgcagccgcg
     2461 cccgccacag gctgggtttg ttcactcatt gccataggaa agtcactttt tcagggttgc
     2521 gatgtaaaga atgatcttat ttgtgattat tacccgacta acatacctgt atgcgtcgtc
     2581 tgaaggaagt ctcaacgccg aatacagaat ttctaatctg gatgcagatt tatcttcacc
     2641 ggacgcagac ttgtctatga tgtcgcgtca tactattttt caacacgttg aaatcaggtc
     2701 agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt aaccacattt
     2761 acactggcga gtgaaactgc tctggcgtat gagcaggata aaacctacaa aattacagtt
     2821 ctgcatacca atgatcatca tgggcatttt tggcgcaatg aatatggcga atatggtctg
     2881 gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg ttgcggctga aggcggtagc
     2941 gtgctgctac tttccggtgg cgacattaac actggcgtgc ccgagtctga cttacaggat
     3001 gccgaacctg attttcgcgg tatgaatctg gtgggctatg acgcgatggc gatcggtaat
     3061 catgaatttg ataatccgct caccgtatta cgtcagcagg aaaagtgggc caagttcccg
     3121 ttgctttccg cgaatatcta ccagaaaagt actggcgagc gcctgtttaa accgtgggcg
     3181 ctgtttaagc gtcaggatct gaaaattgcc gttattgggc tgacaaccga tgacacagca
     3241 aaaattggta acccggaata cttcactgat atcgaatttc gtaagcccgc cgatgaagcg
     3301 aagctggtga ttcaggagct gcaacagaca gaaaagccag acattattat cgcggcgacc
     3361 catatggggc attacgataa tggtgagtac ggctctaacg caccgggcga tgtggagatg
     3421 gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc gcaagatccg
     3481 gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg tgccgggtac gccatgcaaa
     3541 ccagatcaac aaaacggcat ctggattgtg caggcgcatg agtggggcaa atacgtggga
     3601 cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg ttaactacca gctgattccg
     3661 gtgaacctga agaagaaagt gacctgggaa gacgggaaaa gcgagcgcgt gctttacact
     3721 cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat caccgttcca gaacaaaggc
     3781 aaagcgcagc tggaagtgaa aataggcgaa accaatggtc gtctggaagg cgatcgtgac
     3841 aaagtgcgtt ttgtacagac caatatgggg cggttgattc tggcagccca aatggatcgc
     3901 actggtgccg actttgcggt gatgagcgga ggcggaattc gtgattctat cgaagcaggc
     3961 gatatcagct ataaaaacgt gctgaaagtg cagccattcg gcaatgtggt ggtgtatgcc
     4021 gacatgaccg gtaaagaggt gattgattac ctgaccgccg tcgcgcagat gaagccagat
     4081 tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg cgaaagacgg caaactgaac
     4141 gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat ggcgacatta
     4201 aacttcaatg ccaccggcgg tgatggatat ccgcgccttg ataacaaacc gggctatgtg
     4261 aataccggct ttattgatgc cgaagtgctg aaagcgtata tccagaaaag ctcgccgctg
     4321 gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc agtaatccga aagtgccgga
     4381 tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt tggcatcgag
     4441 gatctttgcc agatcgcctg ccgccagttc gatatccagt ccgcgcttgc cgccggaaac
     4501 ataaatagtg gcaaattctt gtgcgggggc gtcgataatc gttggcagac gttttttctg
     4561 ccccagtggg ctaatccccc caaccaggta tcccgtcgaa cgctgcgcga ccatcggatc
     4621 ggccatctca actttcttgg cacccagcgc ttttgctact tttttaagat ccagttgacc
     4681 tgcgaccggc gtaacggcca cggcaaggtg tttcatatca ccgttcactg ccaccagcag
     4741 cgttttgtag acctgatccg gattcaaacc taattttttg acgacttcat cgccaaaatt
     4801 ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt ttttttcgag
     4861 taatttaact gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac gaagcaagca
     4921 tacgcctgat ggcggtccaa aaaaaagagt catcttgcct aagagtattg gcaggatggt
     4981 gagattgagc gacaatcgag ttacaccgtc gcttaaagtg acggcataat aataaaaaaa
     5041 tgaaattcct ctttgacggg ccaatagcga tattggccat ttttttagcg caacatttgc
     5101 ggcaaattcc cttctccata caggtgtagt gcaccgaccg cgaccacata tcgccccggc
     5161 ggcatggcgc gtaatttatc ccgccaggcg agatttcgct gatgcatcag cacatcgtac
     5221 agcgactgac tgaacgtatt gggcagcgtt atatcattat tttgcggcgg tgcattcagc
     5281 caccagctca tcatttgttg cagcaaccgt gcgttggtat gccagtgggt cagcgtatcg
     5341 tccagcagcg ccagtccttt gtcagggagc tggagcaaca tggcaatctg gttttcagcc
     5401 ccttccagtt caatcacggg tttatgttgt tgcttcgccg cctgcaatag ctggtaatcg
     5461 ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag aaccatcgcg
     5521 atttgccaca gcggttgggt agaaaagagt gagggagaaa tgcccatctc ctggctaatg
     5581 tgctgcaggt tttggagttg ctcctcgcta atgcgctctt ccagcgcctc gcaggcaggc
     5641 aaattagcaa aaggcgtatc gctggtggaa acatccgcct cgacgatcag cgcatcggcg
     5701 tttttgagct ttttgagcaa acgggtgggc aggggagcca tatcgtggct acccatatga
     5761 atactgccaa tcagatgaaa atggcgattg ccggggaggg tgatatcgat ggcaggccag
     5821 gtgtaatgat taccgcgcag cgcggcccaa agcgttttta cccggtacaa cagatccata
     5881 cgacctccct ttgtgaaata tcatgctagc gcgcggtgag ggatggcgca aatggagttg
     5941 gggacttgtt taatgtgttt gtatgattca gtatgttctt gcatcgctat tcacagggaa
     6001 gcaacagtta aaaaccatga aacaggcaac aagaaaaccg acgacacccg gagatattct
     6061 tctctatgaa tatctggaac cgctcgattt gaaaatcaat gagttagcag agttgctgca
     6121 tgttcatcgt aatcgcgtca gtgcactgat caataacaat cgtaaactca ccactgagat
     6181 ggcattccgt ctggcgaaag tttttgatac cacagtcgat tttaggctaa acctccaggc
     6241 ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc caggaagaat tgggacggat
     6301 tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg tcgcgtaagg
     6361 ccaaaacatc gcttcatctc gtaggccgga taaggcgttc acgccgcatc cggcatccga
     6421 gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc ctacaaacct gtgcctgaac
     6481 cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca tgcgtcgatg ccaaatgcgc
     6541 cgccctaaag cagcgcatcc gcaatgacgt acttattcct tcggtttaaa ccgcagcaac
     6601 cggttggcgt tactcactac ggtaatcgac gagagcgcca ttgccgctcc ggcaactacc
     6661 gggttaagca gtgttccagt aaacggccac aaaataccgg cggcgaccgg aataccgatg
     6721 ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt tgcgcagcgt tgcgcgggaa
     6781 atcgcgagcg catccgcaac gcccatcagg ctatggcgca tcagggtaat cgccgcggtt
     6841 tcaatggcaa catcactgcc gccacccatc gcaatgccga catccgcctg ggccagcgct
     6901 ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac gtccttcact ttgcagacgt
     6961 ttgatcgctt cggctttacc gtccggcagc actccggcaa tcacttcatc aattccagct
     7021 tctttggcga tcgcattggc ggtggttggg ttatccccgg tcaacatcac cagacgatat
     7081 cccgctttat gcaggcgttg cagcgccgcc acgctatcac tacgcaacgg atcgcgtaca
     7141 gccagcaggg ctactgcttt cccgtcaatc gccagcagca caggcgttgc tccttgcgat
     7201 gcctgagctg aaatatcggc ttcaatcgct ttagtatcga cttgctgatc atttaacaac
     7261 gcctgattac ccagcaataa cgcatgacct tctgcttcac cgctcacgcc cagcccgcgc
     7321 aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc ctgctttatc aaggatcgct
     7381 cgtgccaatg gatggctgga accttgctcc agcgccgccg ccagacgcag cgcctgcgct
     7441 tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg gcttcccttc ggtcagcgtc
     7501 ccggttttat cgaacacgac ggtatcgagt gtactggcgc gttgcagcgc gtcggcgtcc
     7561 cgcaccagca cgccaaactc agccgcccgc ccgacgccgg aaataatcga catcggcgtc
     7621 gccagtccca gcgcacacgg gcaggcgata atcagcaccg tagtggcaat caccagggta
     7681 tagacaatct gcggtgccgg accaaagaaa taccagattg ccgcactgac aagcgcaata
     7741 accactacta ccggcacaaa tacggctgag attttatccg ccagctgacc gatttctggc
     7801 ttgctgctct gggcctggcg caccatgcga atgatccttg atagcgtggt atggctgcca
     7861 accgcactgg cacgaaacag cacactgccg tcctgtacca ctgtcccggc atggacgcta
     7921 tcgccttcgc ctttttgctg cgggattggt tcgcccgtca gcatcgcttc atccagccat
     7981 gcttcgcccc gggtaatctc gccatctacc ggcacgcgat cgccggtcgt caggcgcagc
     8041 aacatacctg gctgcacttc tgccagaggc acgctttttt caccttcgtc agtaaccagg
     8101 cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg ccttagaaga acgctgacgt
     8161 gcgcgtgctt ccagcatatg gccgagattg atcagaccga taatcatcgc actggcttca
     8221 tagtaaagat gtcgcgcttc catcgggaac cactgcggcc acaggttgac gctcatcgaa
     8281 tagagccacg ccacgcccgt tcccagcgcc accagcgtat ccatcgtcgc tgcaccgttc
     8341 agcaggcttt tccatgcact gcggtaaaaa tggccgccgg cgaaaaccat caccgccagg
     8401 gttatcaggc cgataaccaa ccacaggctg cggttgtcgg cggtgaccat catgttatcg
     8461 ccgatcatcc cccagaccat caccgggata cccaccgcca gtgcgacaat tgcctgccag
     8521 cggaagcgct tcatcgtagc gacggcggtt tcttgctggc gttcgcggcg tttcgcgtca
     8581 tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg cctgcactaa atcttgtggg
     8641 gaggcactgc ccatcaccag cgcagtacgc tccgccaggt ttacccgtgc ctgagtgacg
     8701 cccggtacgc tttgcagcgc attttgtacg cgggtgacac agctggcgca gctcatgccg
     8761 ctcagcagca actgctggct gtcatcgtca tcggcggtcg ctgccggaag cgcctcagaa
     8821 accgctgtca gtgcttccga cgggattgat gactccgcca gcggtttagc ctttgggtgg
     8881 cttacagatg cgtcataacc cgcttgtttg atggtttcga tcagctgttc tgcactggca
     8941 gtcccggtaa cgtgcgcttc agtgatagac acatccgctt gttccacatc cgggcgctgt
     9001 tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg acaggccgtc caggttcagg
     9061 tcgatagttt gtgacataaa acactccttt aagacagttt tgactggctg tgataaaggt
     9121 taaaccttcc agcaagggga aggtcaagaa attaataaac caggcgggta aaagtccgta
     9181 aagattaaaa aatcggctcg atttgcatca ggattagaca tttatttctt tgttttcctg
     9241 tagttaagtt gcgggtgcta agttaaatca ggatgcctga aaatcggcac cggggtgagg
     9301 aattacctcc cgcatctata aaaaggggtt aacaaaagat gttagatgca aacaaattac
     9361 agcaggcagt ggatcaggct tacacccaat ttcactcact taacggcgga caaaatgccg
     9421 attacattcc ctttctggcg aatgtaccag gtcaactggc ggcagtggct atcgtgacct
     9481 gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg ctttgcactg gaatccatct
     9541 ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg cccgcaggcg gtacaggaca
     9601 aaattggcgc tgacccgacc ggattgccct ttaactcagt tatcgcctta gagttgcatg
     9661 gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat tgccaccacc agcctgatta
     9721 acgctgaaaa cgccgaacaa cgctggcagc gaattttaca tatccaacag caactggctg
     9781 gtgagcaggt agcgctctct gacgaagtca accagtcgga acaaacaacc aacttccata
     9841 accgggccat tgcctggctg ctgtactccg ccggatatct ctattgtgat gcaatggaag
     9901 cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa tactgttgaa ctggcaacgc
     9961 ttggcgcgac gctggcggcg ggtggtgtga atccgttgac gcataaacgc gttcttcagg
    10021 ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga agggctgtat ggtcgctccg
    10081 gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg tgtaggtggc ggtattctgg
    10141 cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc accgctggac gaagaaggca
    10201 atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa gcaactcggc tataacgtgt
    10261 ttaagggctg atcatgatga acacggaagg taataacggt aacaaacccc tcggtctatg
    10321 gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg gggatcttcg cgctgctggg
    10381 gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc tttgcttttg gcggtattgt
    10441 ggcgatgttt tccggttatg cctatgcgcg tctgggggcg agctatccct gcaatggcgg
    10501 tattatcgac ttttttcgtc gcggattagg caacggcgtc ttttcgctag cgctctcgtt
    10561 gctatacctg ttgacgctgg cggtgagcat cgccatggtt gcccgtgctt ttggcgctta
    10621 tgccgtgcag tttttgcatg aaggcagcca ggaggagcac cttattttgc tctacgcgtt
    10681 ggggatcatt gcggtgatga cgcttttcaa ctccttaagc aaccatgcgg tagggcggct
    10741 ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta ttgccggtgt
    10801 ctggtcgctg caaccggcgc atatttccgt ctctgcgccc cccagctccg gtgcgttctt
    10861 ctcctgtatt gggataacct tccttgccta tgcgggcttt ggcatgatgg cgaacgcggc
    10921 ggataaagtg aaagatccgc aggtcattat gccacgggcg tttctggtgg cgattggcgt
    10981 taccacgttg ctttatatct cgctggcgct ggttttgctt agcgatgtat cggcattaga
    11041 gttagaaaaa tatgctgata ccgccgtagc gcaggctgct tctccgctgc tcgggcatgt
    11101 gggttatgtg atcgtcgtca tcggcgcttt actggcgacg gcttcaggca ttaacgcgaa
    11161 cctgttcgcc gtgtttaaca tcatggacaa catgggcagc gaacgcgaac tgccgaagct
    11221 aatgaataaa tccctgtggc ggcagagtac ctggggcaac atcatcgtcg tggtgttgat
    11281 aatgctgatg acggcggcgc taaatttaga ctcactcgcc agcgttgcca gcgccacctt
    11341 tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg atattcacgc
    11401 ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg ttggctttat
    11461 ctacagcctg tggtcccagg gcagccgtgc gttgatatgg attattggtg caatcttact
    11521 cagccttatt gtggcaatgg tcatgaagcg taataaaacc gtataacacc tctctgtgcg
    11581 cagtacttcc tgtattattg tggtggcggt cgatattcgc actggcaaaa aaacgtgctt
    11641 gaatatctgt tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg atgaacatca
    11701 gcgatgtagc aaaaattacc ggcctgacca gcaaagccat tcgcttctat gaagagaagg
    11761 ggctggtgac gccgccgatg cgcagcgaaa acggttatcg cacctacacg cagcagcatc
    11821 tcaacgaact gaccttactg cgccaggcac ggcaggtggg ctttaacctg gaagagagcg
    11881 gcgagctggt gaatctgttt aacgacccgc agcggcacag cgccgacgtc aaacggcgca
    11941 cgctggagaa ggtggcggag atcgaacgac acattgagga gctgcaatcc atgcgcgacc
    12001 agctgctggc actggcgaat gcctgccctg gcgatgacag cgccgactgc ccgattatcg
    12061 aaaatctctc cggctgctgt catcatcggg cagggtgatt aagacgagac ggctcggata
    12121 tgtagggtta tcccttctat cgcaatgact tcaacatgcg tacctgcacc gagatcctcg
    12181 ctggcgctga caggccatga actgtcaccg acgcgcatat gaccgcgccc gttgaccagc
    12241 ggagattcca gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt taaatgacta
    12301 tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt tccaccacag ccaggcggcg
    12361 agcagcgtca ggatggcaaa cattacccct tgccactccc aacccagcgg caccagccag
    12421 accaccaggc cggtaatcac cgctgccacg ccgctcc
//
LOCUS       DBKEKI010000119        12238 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.119, whole genome shotgun sequence.
ACCESSION   DBKEKI010000119 DBKEKI010000000
VERSION     DBKEKI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.119"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..203
                     /gene="xylF"
                     /locus_tag="ACUQPX_003233"
     CDS             <1..203
                     /gene="xylF"
                     /locus_tag="ACUQPX_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="periplasmic substrate-binding component of the
                     ATP-dependent xylose transport system; high affinity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-xylose transporter subunit XylF"
                     /protein_id="HHV9057841.1"
                     /translation="KPITLLANTAAEIAVELGNGQEPKADTSLNNGLKDVPSRLLTPI
                     DVNKNNIKDTVIKDGFHKESEL"
     gene            281..1822
                     /gene="xylG"
                     /locus_tag="ACUQPX_003234"
     CDS             281..1822
                     /gene="xylG"
                     /locus_tag="ACUQPX_003234"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HHV9057842.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            1800..2981
                     /gene="xylH"
                     /locus_tag="ACUQPX_003235"
     CDS             1800..2981
                     /gene="xylH"
                     /locus_tag="ACUQPX_003235"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HHV9057843.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            3059..4237
                     /gene="xylR"
                     /locus_tag="ACUQPX_003236"
     CDS             3059..4237
                     /gene="xylR"
                     /locus_tag="ACUQPX_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HHV9057844.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HTEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(4345..5169)
                     /locus_tag="ACUQPX_003237"
     CDS             complement(4345..5169)
                     /locus_tag="ACUQPX_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HHV9057845.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            5489..7519
                     /gene="malS"
                     /locus_tag="ACUQPX_003238"
     CDS             5489..7519
                     /gene="malS"
                     /locus_tag="ACUQPX_003238"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HHV9057846.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            7697..8950
                     /gene="avtA"
                     /locus_tag="ACUQPX_003239"
     CDS             7697..8950
                     /gene="avtA"
                     /locus_tag="ACUQPX_003239"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HHV9057847.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRANGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(9101..9574)
                     /locus_tag="ACUQPX_003240"
     CDS             complement(9101..9574)
                     /locus_tag="ACUQPX_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HHV9057848.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(9676..10524)
                     /gene="yiaJ"
                     /locus_tag="ACUQPX_003241"
     CDS             complement(9676..10524)
                     /gene="yiaJ"
                     /locus_tag="ACUQPX_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HHV9057849.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            10725..11723
                     /gene="yiaK"
                     /locus_tag="ACUQPX_003242"
     CDS             10725..11723
                     /gene="yiaK"
                     /locus_tag="ACUQPX_003242"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HHV9057850.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            11735..12202
                     /locus_tag="ACUQPX_003243"
     CDS             11735..12202
                     /locus_tag="ACUQPX_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HHV9057851.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGQEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
BASE COUNT         3088 a         3066 c         3069 g         3015 t
ORIGIN      
        1 ataaacctat tacattgctg gcaaatactg ccgcagaaat tgccgttgaa ttgggcaatg
       61 gtcaggagcc aaaagcagat acctcactga ataatggcct gaaagatgtc ccctcccgcc
      121 tcctgacacc gatcgatgtg aataaaaaca acatcaaaga tacggtaatt aaagacggat
      181 tccacaaaga gagcgagctg taagcgttac gccccggcgc ggagcggggg cgtgatttct
      241 ctccatgccg cgtgaatgaa ttggcttagg tggagtcgtt atgccttatc tacttgaaat
      301 gaagaacatt accaaaacct tcggcagtgt gaaggcgatt gataacgtca gcttgcggtt
      361 gaatgctggc gaaatcgtct cactctgtgg ggaaaatggg tctggtaaat caacgctgat
      421 gaaagtgctg tgtggtattt atccccatgg ctcctacgaa ggcgaaatta tttttgcggg
      481 agaagagatt caggcgagtc acatccgcga taccgaacgc aaaggtatcg ccatcattca
      541 ccaggaattg gccctggtga aagaattgac cgtgctggaa aatatcttcc tgggtaacga
      601 aataacccac aatggcatta tggattatga cctgatgacg ctacgctatc agaagctgct
      661 cgcacaggtc agtttatcca tttcacctga tacccgcgtt ggcgatttag ggcttgggca
      721 acaacaactg gttgaaattg ccaaggcact taataaacag gtgcgcttgt taattctcga
      781 tgaaccgaca gcctcattaa ctgagcagga aacgtcggtt ttactggata ttattcgcga
      841 tctacaacag cacggtatcg cctgtattta tatttcgcac aaactcaacg aagtcaaagc
      901 gatttccgat acgatttgcg ttattcgcga cggacagcac attggtacgc gtgatgctgc
      961 cggaatgagt gaagacgata ttatcaccat gatggtcggg cgagagttaa ccgcgcttta
     1021 ccctaatgaa ccacatacca ccggagatga aatattacgt attgaacatc tgacggcatg
     1081 gcatccggtc aatcgtcata ttaaacgagt taatgatgtc tcgttttccc tgaaacgtgg
     1141 cgaaatactg ggtattgccg gactcgttgg tgccggacgt accgagacca ttcagtgcct
     1201 gtttggcgtg tggcccggac aatgggaagg aaaaatttat attgatggca aacaggtaga
     1261 tattcgtaac tgtcagcaag ccatcgccca ggggattgcg atggtacccg aagacagaaa
     1321 gcgcgacggc atcgttccgg taatggcggt tggtaaaaat attaccctcg ccgcactcaa
     1381 taaatttacc ggtggcatta gccagcttga tgacgcggca gagcaaaaat gtattctgga
     1441 atcaattcag caactcaaag ttaaaacgtc gtcccccgac cttgctattg gacgtttgag
     1501 cggcggcaat cagcaaaaag cgatcctcgc ccgctgtctg ttacttaacc cgcgcatcct
     1561 cattcttgat gaacctacca ggggtattga tattggcgcg aaatatgaga tctacaaatt
     1621 aattaaccaa ctcgtccagc agggtattgc cgttattgtc atctcttccg aattacctga
     1681 agtgctcggc cttagcgatc gtgtactggt gatgcatgaa gggaaactaa aagccaacct
     1741 gataaatcat aacctgactc aggagcaggt gatggaagcc gcattgagga gcgaacatca
     1801 tgtcgaaaag caatccgtct gaagtgaaat tggccgtacc gacatccggt ggcttttctg
     1861 ggctgaaatc actgaatttg caggtcttcg tgatgattgc agctatcatc gcaatcatgc
     1921 tgttctttac ctggaccacc gatggtgcct acttaagcgc ccgtaacgtc tccaacctgt
     1981 tacgccagac cgcgattacc ggcatcctcg cggtaggaat ggtgttcgtc ataatttctg
     2041 ctgaaatcga cctttccgtc ggctcaatga tggggctatt aggtggcgtc gcggcgattt
     2101 gtgacgtctg gttaggctgg cctttgccac ttaccatcat tgtgacgttg gttctgggac
     2161 tgcttctcgg tgcctggaac ggatggtggg tcgcgtaccg caaagtccct tcatttattg
     2221 tcaccctcgc gggcatgttg gcatttcgcg gcatactcat tggcatcacc aacggcacga
     2281 ctgtatcccc caccagcgcc gcgatgtcac aaattgggca aagctatctc cccgccagta
     2341 ccggcttcat cattggcgcg cttggcttaa tggcttttgt tggttggcaa tggcgcggaa
     2401 gaatgcgccg tcaggctttg ggtttacagt ctccggcctc taccgcagta gtcggtcgcc
     2461 aggctttaac cgctatcatc gtattaggcg caatctggct gttgaatgat taccgtggcg
     2521 ttcccactcc tgttctgctg ctgacgttgc tgttactcgg cggaatgttt atggcaacgc
     2581 ggacggcatt tggacgacgc atttatgcca tcggcggcaa tctggaagca gcacgtctct
     2641 ccgggattaa cgttgaacgc accaaacttg ccgtgttcgc gattaacgga ttaatggtag
     2701 ccatcgccgg attaatcctt agttctcgac ttggcgctgg ttcaccttct gcgggaaata
     2761 tcgccgaact ggacgcaatt gcagcatgcg tgattggcgg caccagcctg gctggcggtg
     2821 tgggaagcgt tgccggagca gtaatggggg catttatcat ggcttcactg gataacggca
     2881 tgagtatgat ggatgtaccg accttctggc agtatatcgt taaaggtgcg attctgttgc
     2941 tggcagtatg gatggactcc gcaaccaaac gccgttcttg attttgataa aaattttctc
     3001 aaagccggtt acgtattacc ggttttgagt ttttgcatga ttcagcagga aaagaaccat
     3061 gtttactaaa cgtcaccgca tcacattact gttcaatgcc aataaagcct atgaccggca
     3121 ggtagtagaa ggcgtagggg aatatttaca ggcatcacaa tcagaatggg atattttcat
     3181 tgaagaagat ttccgcgccc gcattgataa aatcaaggac tggttaggag atgtcgtcat
     3241 tgccgacttc gacgacaaac agatcgagca agcgctggct gatgtcgacg tccccattgt
     3301 tggggttggc ggctcgtatc accttgcaga aagttaccca cccgttcatt acattgccac
     3361 cgataactat gcgctggttg aaagcgcatt tttgcattta aaagagaaag gcgttaaccg
     3421 ctttgctttt tatggtcttc cggaatcaag cggcaaacgt tgggccactg aacgcgaata
     3481 tgcatttcgt cagcttgtcg ccgaagaaaa gtatcgcgga gtggtttatc aggggttaga
     3541 aaccgcgcca gagaactggc aacacgcgca aaatcggctg gcagactggc tacaaacgct
     3601 gccaccgcaa accgggatta ttgccgttac tgacgcccga gcgcggcata ttctgcaagt
     3661 atgtgaacat ctacatattc ccgtaccgga aaaattatgc gtgattggca tcgataacga
     3721 agaactgacc cgctatctgt cgcgtgtcgc cctttcttcg gtcgctcagg gcgcgcggca
     3781 aatgggctat caggcggcaa aactgttgca tcgattatta gataaagaag aaatgccgct
     3841 acagcgaatt ttggtcccac cagttcgcgt cattgaacgg cgctcaacag attatcgctc
     3901 gctgaccgat cccgccgtta ttcaggccat gcattacatt cgtaatcacg cctgtaaagg
     3961 gattaaagtg gatcaggtac tggatgcggt cgggatctcg cgctccaatc ttgagaagcg
     4021 ttttaaagaa gaggtgggtg aaaccatcca tgcaatgatt cataccgaga agctggagaa
     4081 agcgcgcagt ctgctgattt caaccacctt gtcgatcaat gagatatcgc aaatgtgcgg
     4141 ttatccatcg ctgcaatatt tctactctgt ttttaaaaaa gcatatgaca caacgccaaa
     4201 agagtatcgc gatgtaaata gcgaggtcat gttgtagcga tgatgagaat tgtcggcgtc
     4261 acatcaggga actcagcaca gccacctgat gcgagcaaaa gccttatccg accgacaaat
     4321 cagataaggc tcgaggaaat gaaattacat atgcgccgcg attaaccgtt ggttatcttg
     4381 gtacattgcg aacaggtagt tgttataact cttccctttg gtcgagtagc ccttcagctt
     4441 gtgaatcatc gctgtggcag tcacttcctg atccgcttta cgcagctgtg cacgcgattt
     4501 acggaacgaa gagtaagccg ggtgcgtatt caggttagtg acataggcgc tcaccgattc
     4561 tttgacagaa ctaaactgtg agtacccttt cactttaccc ggcgcattgg tacaacgtcc
     4621 tttcatgcat ttcatgccga acagattgtt attgttgcgc gccagcttcg acgttcccca
     4681 accgctttct gctgcagcca tcgtcgccac catactggta gggataatgt ctacgcgttc
     4741 aagcaaggta ttccacggga tttttcgcgt attaccagac cacttcacct tgtagcgttt
     4801 ggcgatgtct ttcagacgcg cacgctcagc aggtgaccat tggccctgat actgttttga
     4861 aatcagccag ttacgttctg cagtaatggc tgcattttgg ctggtaatgt aaggcattac
     4921 ggtccggaga aacgcctttt tccttggtgt tccggaaggg tattttcgca aatcaggaag
     4981 tgaactactc tttgcactat tgcgagaata ctcttgttta ctgcttacct gtttattact
     5041 agctttagtt aagtgggact tttgactcgc tgttgttgtg tgcgtcttcg ctaacacctc
     5101 actcgaaaac accagagtga gtaacataag aatcatcgcc ccatatcgtc gtatgggagt
     5161 caaaatcatc aggtctcctg gtcggattta atcattccaa caccttatat ttttcacaaa
     5221 tttgagagtt gaatctcaaa tcatatcaaa aatagctgtc aagagcaccc caaggaatag
     5281 tccaaatctg aaactatgtc acgagttaac gattcagatt ggcgctaaat cgcagaaaat
     5341 gtgggggtta tcgcaaaatt cagccgtttt ttgcgcgcga tcgctcaccc ttgcttctca
     5401 tcctgtggac ttaccgctca gggatgagtt ttgtctggct tatcgctggc aaactgtctg
     5461 aaatcgcagc aataaggact catccaccat gaaactcgcc gcctgttttc tgacactcct
     5521 tcctggcttc gccgttgccg ccagctggac ttctccgggg ttccctgcct ttagcgaaca
     5581 gggaacggga acatttgtca gccacgcgca gttgcccaaa ggtacgcgtc cactcacgct
     5641 aaattttgac caacagtgct ggcagcctgc agatgcgata aaactcaatc agatgctttc
     5701 cctgcaacct tgtagcaaca cgccgcctca atggcgattg ttcagggacg gcaaatatac
     5761 gctgcaaata gacacccgct ccggtacgcc aacattgatg atttccatcc agaacgccgc
     5821 cgaaccggta gcaaacctgg tccgtgaatg cccgaaatgg gatggattac cgctcacgct
     5881 ggatgtcagc gccactttcc cggaaggagc cgccgtacgg gattattaca gccagcaaat
     5941 tgcgatagtg aagaacggtc aaataacgtt acaacccgct gctaccagca acggtttact
     6001 cctgctggaa cgggcagaaa ctgacacatc cgcccctttc gactggcata acgccacggt
     6061 ttactttgtg ctgacagatc gtttcgaaaa cggcgatccc agtaatgacc agagttacgg
     6121 acgtcataaa gacggtatgg cggaaattgg cacttttcac ggcggcgatt tacgcggcct
     6181 gaccaacaaa ctggattacc tccagcagtt gggcgttaat gctttatgga taagcgcccc
     6241 atttgagcaa attcacggct gggtcggcgg cggtacaaaa ggcgatttcc cgcattatgc
     6301 ctaccacggt tattacacac aggactggac gaatcttgat gccaatatgg gcaacgaagc
     6361 cgatctacgg acgctggttg atagcgcaca tcagcgcggt attcgtattc tctttgatgt
     6421 cgtgatgaac cacaccagct atgccacgct ggcggatatg caggagtatc agtttggcgc
     6481 gttatatctt tctggtgacg aagtgaaaaa aacgctgggt gaacgctgga gcgactggaa
     6541 acctgccgcc gggcaaacct ggcatagctt taacgattac attaatttca gcgacaaaac
     6601 aggctgggat aaatggtggg gaaaaaactg gatcagaacg gatatcggcg attacgacaa
     6661 tcctggattc gacgatctca ctatgtcgct ggcctttttg ccggatatca aaaccgaatc
     6721 aactaccgct tctggtctgc cggtgttcta taaaaacaaa acggatactc acgctaaagt
     6781 catcgaaggc tttacacctc gcgattactt aacccactgg ttaagtcagt gggtccgcga
     6841 ctatgggatt gatggttttc gggtcgatac cgccaaacat gttgagttgc ccgcctggca
     6901 gcaactgaaa accgaagcca gctccgcgct tcgcgaatgg aaaaaaacta accccgacaa
     6961 agcattagat gacaaacctt tctggatgac cggtgaagcc tggggccacg gcgtgatgca
     7021 aagtgactac tatcgccacg gcttcgatgc gatgatcaat ttcgattatc aggagcaggc
     7081 ggcgaaagca gtcgattgtc tggcgcagat ggatacgacc tggcagcaaa tggcggagaa
     7141 attgcagggt ttcaacgtgt tgagctacct ctcgtcgcat gatacccgtc tgttccgtga
     7201 agggggcgac aaagcagcag agttattact attagcgcca ggcgcggtac aaatctttta
     7261 tggcgatgaa tcctcgcgtc cgttcggtcc tacaggttct gatccgctgc aaggtacacg
     7321 ttcggatatg aactggcagg atgttagcgg taaatctgcc gccaacgtcg cgcactggca
     7381 gaaaatcagc cagttccgcg cccgccatcc cgcaattggc gcgggcaaac aaacgacact
     7441 ttcgctgaag cagggctacg gctttgttcg tgagcatggc gacgataaag tgctggtcat
     7501 ctgggctggg caacagtgac ttttccggct tcccgttcgt cagtacctcg ggaagccgcc
     7561 aaccaggata aaatgtcagc cctaatcagc gttgcgggat aaagcaccgc tcactcttca
     7621 acagaccgat ttgcacccca gcaaatgtag cgttattgtt accttccttg ctacagagtt
     7681 cgacagatat cccgctatga cattctccct ttttggtgac aaatttaccc gccactccgg
     7741 cattacgctg ctgatggaag atctgaacga cggtttacgc acgcctggcg cgattatgct
     7801 cggcggcggt aatccggcgc agatcccgga aatgcaggac tacttccaga cgctactgac
     7861 cgacatgctg gaaagtggca aagcgactga tgcactgtgt aactacgacg gtccacaggg
     7921 gaaaacggag ctactcacac tgcttgccgg aatgctgcgc gagaagttgg gttgggatat
     7981 cgaaccacag aatattgcac taacaaacgg cagccagagc gcgtttttct acttatttaa
     8041 cctgtttgcc ggacgccgtg ccaatggtcg ggtcaaaaaa gtgctgttcc cgctggcacc
     8101 ggaatacatt ggctacgctg acgccggact ggaagaagat ctgtttgtct ctgcgcgtcc
     8161 gaatattgaa ctgctgccgg aaggccagtt taaataccac gtcgattttg agcatctgca
     8221 tattggcgaa gaaaccggga tgatttgcgt ctcccggccg acgaatccaa caggcaatgt
     8281 gattactgac gaagagttgc tgaagcttga cgcgctggcg aatcaacacg gcattccgct
     8341 ggtgattgat aacgcttatg gcgtcccgtt cccgggtatc atcttcagtg aagcgcgccc
     8401 gctatggaat ccgaatatcg tgctgtgcat gagtctttcc aagctggggc tgcctggctc
     8461 ccgctgcggc attatcatcg ccaatgaaaa aatcatcact gccatcacca atatgaacgg
     8521 cattatcagc ctggcacctg gcggtattgg tccggcgatg atgtgtgaaa tgattaagcg
     8581 taacgatctg ctgcgcctgt ctgaaacagt catcaaaccg ttttactacc agcgtgttca
     8641 ggaaactatc gccatcattc gccgctattt accggaaaat cgctgcctga ttcataaacc
     8701 ggaaggagcc attttcctct ggctatggtt taaggatttg cccattacga ccgagcagct
     8761 ctatcagcgc ctgaaagcac gcggcgtgct gatggtgccg gggcacaact tcttcccggg
     8821 actggataaa ccgtggccgc atacgcatca atgtatgcgc atgaactacg taccagagcc
     8881 ggagaaaatt gaggcggggg tgaagattct ggcggaagag atagaaagag cctgggctga
     8941 aagtcactaa aaattgcctg atgcgctacg cttatcaggc ctacatgatt tctgcaatat
     9001 attgaatttg gaagaatttg taggccggat aaggcgttta cgccgcatct ggcataaaca
     9061 aagcgcacta ttccggcgtt gagaaacgcc ggaatacgtt tcaaacaggc tgctgctgta
     9121 gccgatgccg ctgcactttc tcgacatcca tgcactgcaa cgcctttgtc gggcaggctt
     9181 caacacaggc cggccccgtc tcccgatgcc agcacagatc gcacttaatc gccctcgcct
     9241 ttttgcgcga agaaacgacc tccatcgcac caaacgggca agccagcata cagcttttac
     9301 agccaatgca acgtgtttgt tcaacgaaaa tatgcccatg ttcgcggctt atcgcgtcaa
     9361 cagggcagac attcgcgcac ggtgcatctt cacactgatg acaggctact ggcgtggtcc
     9421 agctgtggtc tttaatgaca cgaatacggg aaataaactc gtctggtgac aacgcagcgc
     9481 aatcctgatt ctcctgatgc gacactgcgc aagccacttc acaggtacgg caaccgatac
     9541 atttcgtcgc atccgcaata ataaaccggt tcatgatcac tcctttgcac taaaacagca
     9601 gagtgccaga gagcgcgtcc ctactgcttt gatccaacga aggaaatgtt aaagttttgg
     9661 cacttgtcca aagcgttatg tgatcgcgcc caggtcatcc ctgacagtaa atcccagttc
     9721 attagaaata gcctgcgcgg tttcacgcag tggtttcagg agatttttct ctcccacctg
     9781 tttcagacgt gatgtcgaaa gcgaaatcga cacggcgtac ggcacccgcc catgaatatc
     9841 aaacaccgga acagcaatac aggagacgcc gagttcgttt tcttctctgt ccatcgccgc
     9901 tccgctttca cgaatgtgcg ccagttcgtc gaacatcgcg ggcagctcgg taatggtatt
     9961 gcgggttaac ggctggatct catgctgatg gttttcccag tatgacttca cgtagtccgg
    10021 gtgaccaaac gccatgtaga tcttgcccat tgcggaacag tagagcggca tatgctggcc
    10081 aatataggca cgggttcgca gcatcccggt tgtgggttcc agcttataaa tcaaaatagc
    10141 gtgatcgtct tcgcggctgg agaagttaat ggtttcacca gtggcgatgt tcagtgcctc
    10201 aagatgcgga gcggcgatat gaatgatatt cagcgaagac agcgccttct gcccgacggc
    10261 aataaatttg gtagtcaggc ggtaactccc tgcggcgggc gcggtggtca catagccgca
    10321 ggactgtaat ccctgcaata agcgatggac ggtactctta tttaaaccag ccagctccga
    10381 aagatgcgcc aacggacaac cgtttggata gttgctcaaa atctcaatca gcatcaaccc
    10441 gcgaaacaga ctctggcttc cggctggacg ctctttttcc tgcgccatct cgttctcttt
    10501 ttttcccatc acttctttcc ccattttgtc gcgtcctgat ggtagcgcaa agtgtgccgt
    10561 agttcacgat ctcgacagat aatttataac cacttgattt ttatgtcttt tgaaattcat
    10621 caatcagatt gctttgttaa aaagtgatcg atatatttga aatctagttt cgtatattga
    10681 aattttaagc caaaaaagcg atcaaaaaaa caaggaagcc tgggatgaaa gtgacatttg
    10741 agcagttaaa agcagccttt aatcgggtct taatttcacg cggcgttgac agcgaaacgg
    10801 ctgacgcctg tgcagagatg ttcgcccgca ccaccgaatc cggcgtttat tctcacggcg
    10861 ttaatcgttt ccctcgtttc attcaacaac tggaaaacgg cgatatcatt cctgatgccc
    10921 aacccaaacg tataaccagc ctcggcgcaa ttgaacagtg ggatgcccag cgttcgatcg
    10981 gcaacctgac ggcgaaaaag atgatggatc gcgccattga actggctgcc gatcacggta
    11041 ttggtctggt ggcacttcgt aatgccaacc actggatgcg cggcggcagc tacggctggc
    11101 aggcggcgga aaaaggctat attggcattt gctggaccaa ctccatcgcc gtaatgccgc
    11161 cgtggggcgc aaaagagtgt cgcatcggca ctaacccgct gatcgtcgcc attccttcca
    11221 cgccgatcac catggtcgat atgtcgatgt cgatgttctc ttacggcatg ttagaagtta
    11281 accgcctggc aggccgtcag ctcccggtcg atggtggctt tgatgatgag ggcaatttga
    11341 ccaaagaacc tggcgttatc gagaagaatc gccgcatttt gccgatgggc tactggaaag
    11401 gttctggcat gtcgattgtg ctggatatga tcgctactct cctttccgac ggcgcatcgg
    11461 ttgccgaagt cacccaggac aacagcgacg aatacggcat ttcacaaatt tttattgcca
    11521 ttgaagtgga caagcttatc gacggtccca cccgcgatgc caagctgcaa cgtatcatgg
    11581 attacgttac taccgctgaa cgcgctgacg aaaaccaggc catccgctta cccggccacg
    11641 aatttactac cctgctggcc gaaaaccgcc gtaacggcat cactgttgat gacagcgtgt
    11701 gggccaaaat ccaggcgtta tgaggagata agtcatgatt tttggacata tcgcgcagcc
    11761 caatccgtgc cgtttgcccg ccgccattga aaaggcgctc gattttctgc gcgctaccaa
    11821 cttcaacgtc ctagagccgg gcgttgtcga aatcgacggc aagaatattt atgcgcaaat
    11881 tatcgattta accacacgcg aagcggtgga aaaccgtccg gaagtgcatc gtcggtatat
    11941 cgatattcag tttctggcgt ggggtcaaga gaaaatcggc attgctattg atacaggaaa
    12001 taataaagtc agcgaatcac tattagagca gcgcgatatt attttttatc atgacagtga
    12061 acatgaatca tttatagaaa tgataccggg cagctacgct attttcttcc cgcaggatgt
    12121 tcaccgacca ggttgtattc tgcaaacagc ctctgagata cgaaaaattg tggttaaagt
    12181 cgcattaaca gcgctgaatt aatcaaataa tcgtctacgc cagaacgact gggtaatg
//
LOCUS       DBKEKI010000120        11734 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.120, whole genome shotgun sequence.
ACCESSION   DBKEKI010000120 DBKEKI010000000
VERSION     DBKEKI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.120"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1357)
                     /gene="cusC"
                     /locus_tag="ACUQPX_003244"
     CDS             complement(<1..1357)
                     /gene="cusC"
                     /locus_tag="ACUQPX_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HHV9057852.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEVERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YT"
     gene            1514..2197
                     /gene="cusR"
                     /locus_tag="ACUQPX_003245"
     CDS             1514..2197
                     /gene="cusR"
                     /locus_tag="ACUQPX_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HHV9057853.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2187..3629
                     /gene="cusS"
                     /locus_tag="ACUQPX_003246"
     CDS             2187..3629
                     /gene="cusS"
                     /locus_tag="ACUQPX_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HHV9057854.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKCEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            3779..6016
                     /gene="nrfB"
                     /locus_tag="ACUQPX_003247"
                     /pseudo
     CDS             3779..6016
                     /gene="nrfB"
                     /locus_tag="ACUQPX_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
     gene            6003..8975
                     /gene="nfrA"
                     /locus_tag="ACUQPX_003248"
                     /pseudo
     CDS             6003..8975
                     /gene="nfrA"
                     /locus_tag="ACUQPX_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            8976..9866
                     /locus_tag="ACUQPX_003249"
     CDS             8976..9866
                     /locus_tag="ACUQPX_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HHV9057855.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            10049..10810
                     /gene="envY"
                     /locus_tag="ACUQPX_003250"
     CDS             10049..10810
                     /gene="envY"
                     /locus_tag="ACUQPX_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HHV9057856.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPRLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(10856..10932)
                     /locus_tag="ACUQPX_003251"
     tRNA            complement(10856..10932)
                     /locus_tag="ACUQPX_003251"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10896..10898),aa:Arg,seq:tct)
     gene            11175..>11734
                     /gene="fimZ"
                     /locus_tag="ACUQPX_003252"
     CDS             11175..>11734
                     /gene="fimZ"
                     /locus_tag="ACUQPX_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HHV9057857.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKS"
BASE COUNT         2897 a         3020 c         2996 g         2821 t
ORIGIN      
        1 cggtatacaa ataaatttcg ttaacctgac gggcataatt caggtcaagt aaagtttgtc
       61 gggttgcaaa taaagaacgc tcggcatcca gcacttccag ataacttacc gcgccgtgct
      121 gatataatgc ccgcgcccgt tgcagggtaa tttgcagcga cgccagataa cgctgctggg
      181 cgctgatttg atcgtccagg ctttgacgta atgccagcgc atctgccacc tctttaaagg
      241 cgttctggat tttctgttca taattcacca ccgactgctg ctggcgaatt tcggcgatat
      301 ccagattggc ctggttgcgt ccggcattaa aaatggggat ctcaatttta ggaataaaat
      361 tccacatccc gctgctagcg ttaaataatg atgacagatc gctgctggcg gttgatattc
      421 cgctggtcag gctgatagac gggaaaaatg ccgcacgcgc agcaccaata ttagcattag
      481 ccgccattaa cgcgtgttca gcttccataa tatccgggcg ctgcaataag atttgcgacg
      541 acaagcccgc cggtaattta acgctttgca ggctgccgct gtttactgtc tgcgcttgcg
      601 gtagcttgcc gtagcttccc aataacagtt gcaatgcatt attcgcctgc gccagttccc
      661 cctgacgttt agcgatgtca ctgcgggtac tttctatcac cccgcgagcc tgttccagcg
      721 ccagaacatt gctgctaccg gtcaacagtt gtttttcgac aaacgcatat gactgctgat
      781 aattacgcag cgtttcttcg gctatttgca attgcgcata cgccagctgc tgattgaaat
      841 agctttgcgc gacattagaa accagcaaaa tatgcaccgc gcgctgggct tcctcagtgg
      901 ctaaataatt ttggcgctcg acttcgctca tgttctttaa gcgaccgaaa aaatcgagat
      961 caaagctggc gttaaggcca gtcgagaact cccgcgtcgt ggctgaatcg cctttaagat
     1021 tgccgctcca gctgccgctg ccctcgccat tgagctgtgg gtagcggtcg gcatcggtca
     1081 gacgatattg cgcccgtgcc tcctgcactt tcagcgtcgc catgcgcaaa tcccggttat
     1141 tcaccagcgc ctcgctaatc agcgtcttca cctgattatc aacaaaaaag gtgcgccagc
     1201 ccgcgttctg gtagttatcc gctgcgttaa ccaggccgtt ctggctgagt gagaactgct
     1261 gcggtacggg cattgccgga cgctgataat ccggtgccag tgaacaacca gttagcgcaa
     1321 gggccacaca aaatggcaga agtttacaag gagacatagg ctcataattt ctggtgattt
     1381 tatgtcgcca actttactcg ccaggctctg attttccggt gacaggaaaa tgacaaaatt
     1441 gtcattttgc caataagcga ttgccatctg atcccgctac tctagaattg cccgggcaac
     1501 atgcggagga aatatgaaac tgttgattgt cgaagatgaa aagaaaaccg gagaatactt
     1561 gaccaaaggg ttaaccgaag ccggttttgt ggtcgatttg gccgacaacg ggctgaatgg
     1621 ctaccatctg gcgatgaccg gtgattatga tctgataatc ctcgatatta tgctgccaga
     1681 cgtgaacggc tgggatatcg tgcgcatgtt gcgctccgcc aataaaggga tgccgattct
     1741 gttgcttacc gcgcttggca ccattgaaca tcgcgtcaag gggctggagt tgggggcaga
     1801 tgactacctg gtgaaaccat tcgcttttgc tgaactgctg gcgcgggtgc gcactctcct
     1861 gcggcgcggg gcggcggtga ttatcgaaag tcagtttcag gttgccgatc tgatggtcga
     1921 tctcgtcagc cgcaaagtca cccgcagcgg cacgcgcatc actctgacca gtaaagaatt
     1981 tactctgctg gagttttttc tccgccatca gggcgaagtg ctgccccgct cgcttatcgc
     2041 ctcgcaggta tgggacatga atttcgacag cgacactaac gccattgatg tggcggtgaa
     2101 gcggctacgc ggcaaaatcg acaacgactt tgagccgaag ctgattcaaa ccgtgcgcgg
     2161 cgtgggttac atgcttgagg tgccggatgg tcagtaagcc atttcagcgc ccgttttcgc
     2221 tggcaacccg cctgaccttt tttatcagcc tggccaccat cgcggcgttt ttcgcctttg
     2281 catggatcat gatccactca gtaaaagtgc attttgccga gcaggacatt aatgatttaa
     2341 aagagattag cgccaccctt gaacgggtac taaatcaccc tgacgaaacg caagcccgac
     2401 gcttaatgac gctggaagat atcgtcagtg gttattccaa cgtgttgatt tccctggcag
     2461 atagccacgg caaaacggtg tatcactccc ccggtgcgcc tgatatccgc gagtttgcac
     2521 gtgatgccat acccgataaa gacgcgcggg gtggcgaagt gtatctcctt tccggcccga
     2581 cgattatgat gccaggccac ggtcacgggc atatggaaca cagcaactgg cggatgatta
     2641 acttgccggt tggcccgttg gtggacggca aaccgattta tacgctctac atcgcgctgt
     2701 cgatcgattt tcatcttcat tacataaatg atttgatgaa taaacttatt atgaccgcat
     2761 cggtaatcag catcctgatc gtctttatcg tgctgttggc ggtacataaa ggtcacgcgc
     2821 cgatccgcag cgtcagccgt caaatccaga atatcacctc gaaagatctc gacgttcgcc
     2881 ttgacccgca gaccgtgccg attgagctgg aacagctggt actgtcgttc aaccatatga
     2941 tcgagcgtat tgaggatgtc tttactcgcc agtccaattt ctcagcggat atcgcccacg
     3001 aaattcgcac accaattact aatctcatta cgcaaacgga aatcgccctc agccagtcgc
     3061 gcagccagaa ggagctggaa gatgtgctct actctaatct cgaagagctg acgcgaatgg
     3121 cgaaaatggt cagcgatatg ctgtttctcg ctcaggccga taacaaccag ctaatccccg
     3181 aaaagaaaat gctcaacctg gcggatgaag tcggcaaagt gttcgatttt ttcgaggcgc
     3241 tagcggaaga tcgcggcgtg gagttgcggt ttgttggcga caaacgtcag gttgcgggcg
     3301 atccgttgat gctgcgtcgg gcgctaagca acctgctttc taatgccctg cgttatacgc
     3361 caaccagaga gacaattgta gtgcgctgtc agacggtcga tcaccaggtg caagtttccg
     3421 tcgaaaaccc cggtacgccc attgcgcccg agcacttacc gcgattgttt gaccgtttct
     3481 atcgcgttga cccctcccgc cagcgaaaat gtgaaggtag cggtattggg ctggcgatag
     3541 tgaaatcgat tgttgtcgcg cataaaggca cggttgcggt aacgtcagat gcgcggggga
     3601 caaggtttgt tatcacatta cccgcttaat tattcagcaa acggcaactt ttataaccag
     3661 tgtaaaaata acgtgccgca atagtcatac ctattaatgg taaaaagctg tcacaattca
     3721 taaaaaacct taatatacgc caccctaaac ataaccagcg ttaatgtaag gtttttgtgt
     3781 ggactggctt cttgatgttt ttgctacctg gctctacggc ttaaaagtaa tcgcgataac
     3841 gttagcggtc atcatgttca tcagcgggct ggacgatttt tttattgatg tcgtctactg
     3901 ggtacgccgc attaaacgca agttgagtgt ttatcgccgc tacccgcgaa tgagttaccg
     3961 cgaactgtat aaaccagatg aaaaaccgtt agcgattatg gttccggcgt ggaatgaaac
     4021 gggcgtcatc ggcaatatgg ccgagctggc agcgaccacg ctcgactacg aaaactatca
     4081 tatctttgtt ggcacctacc ccaacgaccc cgatactcaa cgtgatgttg acgaagtgtg
     4141 cgctcgcttt ccgaacgtgc ataaggtagt ctgcgcgcgt cctggtccca ccagcaaagc
     4201 cgactgtctg aacaacgtgc tggacgccat cacccagttt gaacgtagcg ccaatttcgc
     4261 ttttgctggt tttattctgc atgacgccga agatgtgatt tcaccgatgg aattgcgtct
     4321 gttcaactat ctggtcgagc gtaaagatct gattcagatc ccagtgtatc cgtttgaacg
     4381 cgaatggacg cacttcacca gcatgactta cattgatgag ttttcagaac tgcatggcaa
     4441 agatgttccg gtgcgtgaag ccctcgccgg acaagtgccc agcgcaggcg tcggcacctg
     4501 tttcagccgc cgcgccgtga ccgcactgtt agctgacggt aacggtattg ctttcgacgt
     4561 gcagagtctt actgaagatt acgacattgg cttccgcctg aaagaaaaag gtatgacgga
     4621 aatttttgtc cgttttccgg tggtggacga agccaaagaa cgcgagcagc gtaaattttt
     4681 acagcacgca cggacgtcaa acatgatctg cgtgcgcgaa tatttccccg ataccttttc
     4741 gactgcggtt cgacaaaaat ctcgctggat catcggcatt gttttccagg gctttaaaac
     4801 ccataaatgg acctccagcc tgacgctgaa ctactttctc tggcgcgacc gcaaaggggc
     4861 aatcagtaac tttgtcagct tcctcgcgat gctggtgatg atccagcttt tgctgttgct
     4921 ggcgtatgaa agtttgtggc ccgatgcctg gcatttcctt tctattttta gcggcagcgc
     4981 atggttaatg accctgctgt ggctaaactt tggcttgatg gttaaccgca tcgtgcagcg
     5041 agtgattttc gtcactggct actacggcct cacgcagggc ctgctttccg tcctgcgtct
     5101 tttctggggc aacctgatta actttatggc taactggcgc gtgctgaaac aggttcttca
     5161 gcacggcgat ccgcgtcgcg tggcgtggga taaaacaacg catgacttcc ccagcgtcac
     5221 tggcgatacc cgctcgttgc gcccgttagg tcaaattctg ctggaaaatc aggtcatcac
     5281 tgaagaacaa ctcgatacag cactgcgtaa tcgcgtcgaa ggtctacgcc tgggcggttc
     5341 aatgctgatg caggggctga ttagcgccga gcagctggca caggcgctgg cagagcaaaa
     5401 cggcgtggcg tgagaatcca tcgatgcctg gcagatcccg tcctcgctga ttgccgaaat
     5461 gccagcctcc gtagcgctgc attatgcagt actgccgctg cgtctggaaa atgatgagtt
     5521 aattgtcggc agtgaagatg gtattgaccc ggtttcgctg gcggccctga cgcgtaaagt
     5581 cggacgcaaa gtgcgttacg tcattgttct gcggggacaa attgtcacag ggttacgcca
     5641 ctggtatgca cgccgacgcg gtcacgatcc gcgggcaatg ttgtacaatg cggttcagca
     5701 tcagtggctc acggaacagc agaccggtga aatctggcgg caatatgtgc cgcatcagtt
     5761 cctgttcgcc gaaatactga ccacgctcgg tcatattaat cgttcagcaa ttaacgtgtt
     5821 gttattgcgc catgaacgca gttctctgcc gctcggcaag tttttggtca ccgaaggcgt
     5881 tatcagccag gaaacgttgg atcgcgtcct gacaattcaa cgcgaattac aagtttcgat
     5941 gcaatcacta ttactcaaag caggtttaaa cacagaacag gttgcgcaac tggagtccga
     6001 aaatgaagga gaataacctt aatcgcgtca tcggatggtc tggtttactg ctgacgtctt
     6061 tattgagtac cagcgcactc gcagacaata tcggcaccag cgcagaagag ctggggctga
     6121 gcgattatcg ccattttgtt atttatcccc gtctcgataa ggcgctgaag gcacagaaaa
     6181 ataacgacga agcaaccgcc atccgcgaat ttgaatatat acaccagcag gtgccggata
     6241 atattccgct gactttatac cttgcggaag cctatcgtca ttttggtcat gatgaccggg
     6301 cgcggctgtt gcttgaggat caactgaaac gtcacccagg agatgcccga cttgagcgca
     6361 gtctggcggc tattccagtt gaagtgaaaa gcgttacgac tgttgaagaa ctgcttgccc
     6421 agcaaaaagc gggcgacgct gcgccgaccc tgcgttgtcg cagtgaagtc gggcagaatg
     6481 ccctgcggct ggcacaatta cctgtcgcca gagcgcaact gaacgatgcg acgtttgctg
     6541 catcgccgga aggaaaaacg ctgcgaaccg atctgctgca acgggcaatc tacctgaaac
     6601 aatggtccca ggcagatacg ctatacaatg aagcacgcca gcagaacaca ttaagcgcgg
     6661 cagaacgccg tcagtggttt gacgtgcttc ttgccgggca gctggacgat cggatcctgg
     6721 cactgcaatc acaggggatc ttcaccgatc ctcagtcata tattacttac gcgaccgcgc
     6781 tggcttatcg tggcgaaaaa gcacgcctcc agcattatct cattgaaaat aagccgctgt
     6841 ttaccacggg cgcacaagag aaaagttggc tctatctgtt atctaaatac agcgccaacc
     6901 ccgttcaggc attggcgaat tatacggtgc agtttgccga taaccgccag tatgttgttg
     6961 gcgcgacgct accggtgctg ttaaaagaag gtcagtacga cgcagcgcaa aaactgctcg
     7021 ccaccctccc cgccaatgaa atgcttgagg agcgttatgc tgtcagcgtg gcgacccgta
     7081 acaaggctga atctctgcgt ctggcacgat tgctgtatca gcaagaaccg acaaatcttc
     7141 cccgcctgga tcaactgacc tggcaactga tgcagaacga gcagtcacgc gaagctgccg
     7201 atttattgct gcaacgctat cctttccagg gcgatgcgcg tgtcagccag actttaatgg
     7261 cgcgactggc gtctctgctg gaaagtcatc cttacctagc aacgccggcg aaggtggcga
     7321 ttttatcgaa acccttaccg ctggcggagc aacgtcagtg gcaaagtcag ttgccgggta
     7381 ttgcagataa ttgcccggca atagttcgct tgctgggcga tatgtcgcct tcctacgatg
     7441 ctgccgcctg gaaccgtctg gcaaagtgtt atcgggacac gctacccggt gtggcgttgt
     7501 atgcatggct tcaggccgaa caacgacaac cgagcgcctg gcaacatagt gcggtagcct
     7561 atcaggcgta tcaggttgag gactacgcca ccgcactggc ggcctggcag aaaatcagtc
     7621 ttcacgacat gagcaatgag gatctgctcg ccgccgccaa caccgctcag gcagcgggaa
     7681 atggcgcggc tcgcgatcgc tggcttcaac aggcagaaca acgtgggctg ggaaacaatg
     7741 ctcaatactg gtggctgcat gcgcaacgtt atatcccagc tcagccagaa ctcgcactga
     7801 acgatctcac gcgctcaatc aatattgccc cttctgccaa cgcttacgtt gcgcgggcga
     7861 caatttatcg ccaacgtcat aatgttccgg ccgcggtgag tgatttgcgc gccgcgctgg
     7921 aactggaacc gaataatagc aacacccagg cagcgcttgg ttacgccttg tgggatagcg
     7981 gtgatatcgc acagtcgcgg gaaatgctcg aacaggcgca taaagggctt ccggacgatc
     8041 cggcactgat ccgacaactg gcctacgtga accagcgtct ggatgacatg cctgcgacgc
     8101 agcactacgc ccggctggtg attgatgaca ttgataatca ggcgctgata accccactga
     8161 ccccagagca aaatcagcaa cgcttcaatt tccgctgttt gcatgaggag gtcggtcgcc
     8221 gctggacgtt cagtttcgat tcttccatcg gcttgcgttc cggcgcaatg agtaccgcta
     8281 acaataatgt cggcggtgca gcgcctggga aaagctatcg tagctacgga caactggaag
     8341 ccgagtaccg catcggacgc aatatgctgc tggaaggcga cctgctctca gtttatagcc
     8401 gcgtctttgc cgataccgga gaaaacgggg tgatgatgcc ggtgaaaaat ccgatgtccg
     8461 gcaccggtct gcgctggaag ccgctgcgcg atcagatctt tttcctcgcc gtcgaacagc
     8521 agttgccgct gaacgggcaa aatggcgcat ccgataccat gctgcgcgcc agcgcctcat
     8581 tctttaatgg cggcaaatac agcgacgaat ggcacccgaa cggttcaggc tggtttgccc
     8641 aaaacctgta cctcgatgcg gcgcaatata tccgctagga tattcaggcg tggacggcag
     8701 attatcgcgt cagctggcat cagaaggtag ctaacggaca gactattgag ccttacgctc
     8761 acgttcagga caacggctat cgtgataaag gcactcaggg cgcgcagctt ggcggtgtcg
     8821 gggtccgctg gaatatttgg accggcgaga cgcactacga cgcctagccg cacaaagtca
     8881 gtctcggcgt cgagtatcaa cataccttta aggcgattaa tcaacgtaac ggagagcgca
     8941 acaacgcgtt tctcaccatt ggagtacact ggtaaatgcg taagttcatt ttcgtattgc
     9001 tgacactgct tttggtcagc cctttttcct ttgcgatgaa aggtattatc tggcaaccac
     9061 aaaaccgaga tagtcaggtt accgataccc agtggcaggg gctgatgagt cagttacgtt
     9121 ttcaaggctt cgataccctg gttttgcaat ggacccgtta cggcgatgca tttacccagc
     9181 cagaacagcg cacgttattg tttaagcgtg ccgcagctgc gcaacagact ggcctgaagc
     9241 tgattgtcgg gctgaacgcc gatccggaat tttttatgca ccagaaacag tcgtctgcag
     9301 cgctggaaag ctatcttaat cgcctgctgg ctgccgatct ccagcaagcc agattatgga
     9361 gcgccgcgcc tggcataacg ccggatggct ggtacatcag cgcggaaatt gacgacctga
     9421 actggcgcag cgaagccgcc cgtcaacctt tgctaacatg gttaaacaac gcgcagcggc
     9481 tgattagcga tgtttcagca aaaccggttt atatcagtag ttttttcgcc ggaaacatgt
     9541 cgcccgatgg ctatcgccaa ctgctggaac aagttaaagc aaccggagtt aatgtctggg
     9601 tacaggatgg cagcggcgtg gataaactta ccgctgaaca gcgtgaacgt tatctacagg
     9661 ccagcgccga ttgccaaagc tccgccccag caagcggcat tgtttatgaa ctgtttgtcg
     9721 ccggcaaagg caaaaccttt acagcgaaac cgaaaccgga cgcagaaatt gcctcgctgt
     9781 tagcgaaacg ttcctcttgc ggtaaagaca tgctctattt ctctctgcgc tatttgcccg
     9841 tcgcgcacgg cattctcgag tattaaatcc cctccaggta agtcgggtac gacctggctt
     9901 acctctttcg ctctcgtcag aattccatca agattaattg ctaaaaagca tgctaaagaa
     9961 agaagatcgt tatcggcgaa cgggccgatc actcatgcag gaagcataga tcctttgagc
    10021 ggagtttctg atatctgagg agtgcgaaat gcaattgagc agcagtgaac cttgcgtggt
    10081 gatcctgacc gaaaaagagg tagaggtaag cgtcaataac catgctacgt ttacccttcc
    10141 gaaaaactac ctggccgcct tcgcgtgcaa caataacgtc attgaactct caacgttaaa
    10201 tcacgtatta atcacccaca tcaaccgtaa catcatcaac gattatctgt tgtttttaaa
    10261 taagaaatta acctgtgtaa agccctggtc gcggctggca accccggtta tcgcttgtca
    10321 tagccgtaca ccggaagtgt tccggctagc cgccaaccac agcaagcagc aacccagcaa
    10381 accctgcgaa gcggagttga cgcgcgcatt actttttacc gtattgtcta actttcttga
    10441 gcaatcgcgg tttattgccc tactgatgta tatcttacgc agcagcgtcc gcgacagcgt
    10501 ctgccgcatt attcaaagcg atattcagca ttactggaat ctgcgaattg tcgccagttc
    10561 gctatgttta agccccaggc tgctcaaaaa gaaattaaaa aacgaaaata ccagctatag
    10621 ccagattgtc acagagtgtc gtatgcgtta cgccgtacag atgttattga tggataacaa
    10681 aaatatcact caggtggcgc aattatgtgg ctatagcagc acgtcgtact ttatctctgt
    10741 ttttaaggcg ttttacagcc tgacaccgtt gaattatctc gccaaacagc gacaaaaagt
    10801 gatgtggtga agggcaaagc ggaaacggat aagacgggca taaatgaaga agaaatggcg
    10861 cgccctgcag gattcgaacc tgcggcccac gacttagaag gtcgttgctc tatccaactg
    10921 agctaagggc gcgttgatac cgcaatgcgg tgtaatcgcg tgaattatac ggtcaaccct
    10981 tgctgagtca atggcttttg atctggttgc tgaacaagtg aacgaccgcg tctgattttc
    11041 tgatttattt cgctatagcg gcaaacaaac gcacaccgct gcgcgtctga atcaagaaaa
    11101 cccgtatttt catgtatcaa agtacaattt accgccctaa cggaaaattg tccgctccta
    11161 tgagactggt aactatgaaa ccaacgtcgg tgatcattat ggatactcat cctatcatca
    11221 gaatgtctat tgaagttctg ttgcaaaaaa acagtgaatt gcagattgtc ctgaaaacgg
    11281 atgattaccg cataaccatc gattatctcc gaacccgtcc tgttgattta atcattatgg
    11341 atatagactt gcccggaaca gacggtttta ccttcctgaa aaggatcaaa caaatccaga
    11401 gcacagtgaa agtgttattt ttatcatcga aatcagaatg cttttatgct ggcagagcga
    11461 tacaagctgg tgctaacggt tttgtcagta aatgcaatga tcagaatgat atttttcatg
    11521 ccgttcagat gatcctctcc ggatacacgt tttttcccag cgaaacgctt aactatataa
    11581 aaagcaataa atgtagtacg aatagttcaa cggtcactgt gctatctaat cgtgaagtga
    11641 ccatattacg ttatctggtt agcggattat ctaataaaga aattgccgat aagttattgc
    11701 ttagcaataa aacagttagt gcgcataaat ctaa
//
LOCUS       DBKEKI010000121        11730 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.121, whole genome shotgun sequence.
ACCESSION   DBKEKI010000121 DBKEKI010000000
VERSION     DBKEKI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.121"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            195..440
                     /locus_tag="ACUQPX_003253"
                     /pseudo
     CDS             195..440
                     /locus_tag="ACUQPX_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            875..1378
                     /locus_tag="ACUQPX_003254"
     CDS             875..1378
                     /locus_tag="ACUQPX_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9057858.1"
                     /translation="MSNTGKGGGQRVVFNVDIVGSACHVVVDADGFGNSGRLIFGTYR
                     KSSGVPVLPRDFVVRLYESGATVQGCSAFRAGQVATLDFGNPGQLDAKGVVTRGAGDG
                     IRVDVRAVDTQADYRGRLTQDSHSVNYPVEFAVKGQFRFRAQPVFPANVKAGEYTGAL
                     TFVVTYQ"
     gene            1435..3831
                     /gene="faeD"
                     /locus_tag="ACUQPX_003255"
     CDS             1435..3831
                     /gene="faeD"
                     /locus_tag="ACUQPX_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial usher FaeD"
                     /protein_id="HHV9057859.1"
                     /translation="MFSRRAALAVSAAFASTTGVAGEKLDMSFIRGGAGVNPEVWAAL
                     NGSYAPGRYLVDLSLNGKEAGKQILDVTPQDSDELCLTEAWLAKAGIYVSADYFREGY
                     DATRQCYVLTKAPSVKVDFDVSTQSLALSIPQKGLVKMPENVDWDYGTGAFRVNYNAN
                     ANIGRNNTSTFGSADLKANIGHWVVNSSASASGGDSGDNSATINAFTATRAIRALSAD
                     LTVGKTSTGSSLLGSTGTYGVSLSRNNSMKPGNLGYTPVFSGIANGASRVTLTQNGRL
                     LHSEMVPAGPFSITDVPLYTSGDVTMKITGEDGREQTQLFPLSVMAGQLNPGQHEFSV
                     AVGLSDDDSDLKGGVFAASYGYGLDGLTLRTGGVFNRDWQGISAGAVLGLSYLGAVSA
                     DGAYAMAEYRDGSRSGNKVQLSWSKQLEMTNTGLRVSWSRQSEEYEDMSSFDPTELWS
                     QSNHGRRPKDEWNAGISQSVGGLFSLSVSGWQRSYYPAQMTGRYRYSDDNGLDTGITG
                     TLSTQIKGVSLNLGWSGSRNSQGENNRSASASISVPFTLFERRYSSSTSVSTSKGGGT
                     GFSTGVSGSLNDRFSYSLGGGRDSGGGVSSYLNASYSGDRAYLNGALNHSQSGGTSGS
                     VSVSGSVLVVPMAKDIMFSRTTGDTVAVVNVKDTPGVKVTSGDGQTDSDGNLVVPLNS
                     YDWNTVTIDAGTLPLSTELTNTSQKVVPTDKAVVWMPFDALKVKRYLLQVKQRDGEFV
                     PGGTWARDSKNTPLGFVANNGVLMINTVDAPGDITLGQCRIPAARLQDTEKLQEITCE
                     "
     gene            3824..4585
                     /gene="faeE"
                     /locus_tag="ACUQPX_003256"
     CDS             3824..4585
                     /gene="faeE"
                     /locus_tag="ACUQPX_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial chaperone FaeE"
                     /protein_id="HHV9057860.1"
                     /translation="MSKRNAVTTFFTNQVTKALGMTLALMMSCQSAVASLAADQTRYI
                     FRGDKDALTITVTNNDKERTFGGQAWVDNIVEKDTRPTFVVTPSFFKVKPNGQQTLRI
                     IMASDHLPKDKESVYWLNLQDIPPALEGSGIAVALRTKLKLFYRPKALLEGRKGAEEG
                     ISLQSRPDGRTMLVNTTPYIFAIGSLLDGNGKKIAADNETAQKLLMFMPGDEVQVKGN
                     VVKVDSLNDYGELQTWTINQKKSAISSAQKSSDKK"
     gene            4620..5111
                     /gene="faeF"
                     /locus_tag="ACUQPX_003257"
     CDS             4620..5111
                     /gene="faeF"
                     /locus_tag="ACUQPX_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeF"
                     /protein_id="HHV9057861.1"
                     /translation="MKKTMMVVALALSALSIQSTLAAEYSEKAQYLGVVNGQVVGNSV
                     VKVTRIPTDPVLYRSGDTTPLPDRLTIRNAESRAASGGLAYITVKQVLPDNGEARITL
                     KTALMVDGKKVAISARQQGEDMVITLPEAQKQIELRTDAPAELEVPVSYRGNLQIALQ
                     VED"
     gene            5349..6149
                     /locus_tag="ACUQPX_003258"
     CDS             5349..6149
                     /locus_tag="ACUQPX_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cshE pilin"
                     /protein_id="HHV9057862.1"
                     /translation="MKKTLIALTVAASAAVSGSAMAWNPSGLGGNGGTFSMSGTLSKT
                     EKTVPWAVEIGAPVTALDVAVKPDQKNVFILLEKDIPILGIRSNPTGFMGEKGIAPQI
                     DYNKAADVDYMDKGNLPAQVNVTSSDGKKIGTLAFVMRVACQAGEKRVESDDKLESNM
                     LFASSKGKAFFGGVAKDDKGVWSDAAAYTWATHLFSSIANSWDTKIPYTAGDTDETDF
                     SNGDKLYRGYYASGLAKDIPMELVLDQTITSATPVPWKASLPIVISYM"
     gene            6370..7167
                     /gene="faeH"
                     /locus_tag="ACUQPX_003259"
     CDS             6370..7167
                     /gene="faeH"
                     /locus_tag="ACUQPX_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeH"
                     /protein_id="HHV9057863.1"
                     /translation="MKITHHYKFFLSAVILVTIFYSAVPYANILDGGEIQFNGYVTDE
                     APKWAWQISSPDQTWSVDTADARTENGQLVFNLHDKGALPFLEGHLHDVAERGGPGFT
                     PFITFSSNGQPFTVTEGNGASAQHFRASVPVRDPETGNVSGQLFFTLNQGMAVSTGRQ
                     DDGASVPAGMSLVSGQSVTDVQPGSLPQGLKARLSSLLLMNQNFGNGMNAVDNGQVIN
                     QGILTDGRVMDLAAAYASEVSDFELRLPAEGTPAAWQAGLNVTVTVQ"
     gene            7197..7949
                     /gene="faeI"
                     /locus_tag="ACUQPX_003260"
     CDS             7197..7949
                     /gene="faeI"
                     /locus_tag="ACUQPX_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeI"
                     /protein_id="HHV9057864.1"
                     /translation="MKKVTLFLFAASHLPSCVLAWNTPGENFSGELKLEGPVTSTRNP
                     WVWKVGQGNGGLEVKQSGGGGGIQVALPAMTVLLGKTTLTTPAGREGLSPRVSYGKGT
                     EGFSLVWTAPGMAEVTLPVTGDNNARAGTFVFRIQAVGVLRHVQGGQPVYVGMYDDLN
                     TNGLPGESTAMKVSDIPGVLQKMFSGEGPGWLQTMTLSGYSGVSHFSDDFLRQVEGVY
                     GAQTVAGSGELRLNGEMPEHWRVSLSVNIEYQ"
     gene            8274..9500
                     /locus_tag="ACUQPX_003261"
     CDS             8274..9500
                     /locus_tag="ACUQPX_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HHV9057865.1"
                     /translation="MSGTIPKIKTGENVLSAAIQRIEWLFGTFPSVCLSFSGGKDSTV
                     LFHLVSEVARKKRRRFSVLFIDWEAQYQCTITHIQKMQKMYLDVTDRFYWVALPLTTV
                     NGVSQFQPEWICWEPGVTWVRHPPEYAITDMAYFPFYRYAMTFEEFIPAFSSWFARDK
                     YLVAVLTGVRADESLNRFMGLVSRRKQRYADDKPWTTASPEGFYYTMYPLYDWKARDI
                     WIYNARTRAIYNPLYDLMHRACVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCE
                     RVSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKIAV
                     YLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYE
                     RYLQRMKERRKEWGIL"
     gene            9485..10123
                     /locus_tag="ACUQPX_003262"
     CDS             9485..10123
                     /locus_tag="ACUQPX_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071453 - cellular response to oxygen
                     levels [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HHV9057866.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            10351..10743
                     /locus_tag="ACUQPX_003263"
                     /pseudo
     CDS             10351..10743
                     /locus_tag="ACUQPX_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10762..11235
                     /locus_tag="ACUQPX_003264"
     CDS             10762..11235
                     /locus_tag="ACUQPX_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9057867.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
     gene            11345..11497
                     /locus_tag="ACUQPX_003265"
     CDS             11345..11497
                     /locus_tag="ACUQPX_003265"
                     /inference="COORDINATES: protein motif:HMM:NF012464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHV9057868.1"
                     /translation="MELQDWRKEPRKKYLVQIVGSFAEFERAMLRERTKSGLAAARQD
                     GLVDGD"
BASE COUNT         2978 a         2471 c         3303 g         2978 t
ORIGIN      
        1 ggctgagcgc cgcagagggg aagttgcgtc catgttgagg ggaacggagc aaaaaaccat
       61 cagagatata ttgaatcaat caatgtaagc aatatcaagt gtctatacca attacgtgat
      121 gatcaggtcg tgttgaataa acgcgcattc ttcggtgctt ttctaatctt ttggggattt
      181 aagttcctgt cgatgaactg ctggcgctgc ttcccccttt taccatcggt atgttgacca
      241 ctgacgaatg gggtagttac accagagagt taccgaaaga gaagtatctg actggtaaga
      301 tttttactca gcgcattgag cgcaacaatc tgcccctgag aacccgaatc aaacgcctgg
      361 ctcgcagaac catctgtttc tctcgctctg taaaatttca tgaaaaagtt atcggtgctt
      421 ttatcgaaaa acatatgttc ctggagtcat cacccttttc ctcttggtgt tatcccatca
      481 atatttcggc cacgtggttt acgtttatta ttgataataa tcaatgaact gccgagtaaa
      541 aataactgct gatatacggg atcccatgtc aactcccgca ttatatatta tacctcaata
      601 gcgttacatt attaatgtat gtaaatgtta attaaccaaa gcattaatca attgttatat
      661 aattgcgtcg caggtaaacg ccaaatcaac gcaaatataa atagtaaaaa tggttcatgt
      721 tttggcacta tttggttttt ttgtttttaa cgggtggata tttgtttatt ttttttctaa
      781 attattttta tgtaattttc ataaaaatgg ggtgtgagtt attaactcta aattagtgag
      841 tattaaatat attgtgtttg tctctgcttt attaatttcc aatacgggaa aggggggggg
      901 gcagcgagtt gttttcaatg tggatattgt aggttctgcc tgtcatgtgg tggtggatgc
      961 ggacggtttc ggcaacagtg gtcgactgat attcgggact taccgtaaat ccagtggcgt
     1021 gcctgtattg ccacgtgact ttgttgtgcg cttgtatgag tccggcgcta cggttcaagg
     1081 ttgttcagcc tttcgagcgg ggcaggtcgc aactctggat tttggtaacc cgggccaact
     1141 ggatgcaaag ggcgtggtca ctcgcggtgc tggtgatggt attcgcgtgg atgtacgggc
     1201 ggtggataca caggcggatt atcgcggacg tctgactcag gacagtcatt cggtgaatta
     1261 cccggtggag tttgctgtta agggacagtt ccgttttcgt gcacagccgg tgttcccggc
     1321 gaacgtgaag gcaggggaat acacaggggc gctgaccttt gtggtcacgt atcagtaaca
     1381 gaggaaaggt gtgtgtgtgc aggaaagaag gtggttgcag agctggtttc gtctttgttt
     1441 tcccgtcggg ctgcgcttgc tgtatcagct gctttcgcca gtaccacagg tgttgcaggt
     1501 gaaaaactgg atatgtcctt tatccggggg ggggccgggg ttaatccgga agtctgggca
     1561 gcgctgaacg gcagctatgc gccgggacgt tatctggttg acctgtccct gaacgggaag
     1621 gaggccggga aacagatact ggatgtgaca ccacaggaca gtgatgaact gtgcctgaca
     1681 gaggcatggc tggcgaaggc cgggatttac gtcagtgcag attactttcg tgagggatat
     1741 gacgccacac ggcagtgtta tgtgctgaca aaagcgccgt cagtgaaggt ggattttgat
     1801 gtttccaccc agagtctggc gctgtccatt ccccaaaagg ggctggtgaa gatgccggag
     1861 aacgtggact gggattacgg gactggtgca tttcgcgtga actataacgc gaacgccaac
     1921 atcggccgga ataacacctc gacctttggc tcggcagacc tgaaggccaa tatcgggcac
     1981 tgggtggtga attcttctgc ctcagccagc ggtggtgaca gcggggataa ctccgccacg
     2041 ataaacgcgt tcacggccac ccgggccatc cgcgcactga gtgcggacct gacggtcggg
     2101 aaaacatcca ccgggagcag tctgctgggc agtacgggaa cgtacggagt gtcgctgagc
     2161 cggaacaaca gcatgaagcc gggcaatctg gggtataccc cggtgttcag cggcattgcg
     2221 aacggggcgt cgagggtgac actgacacag aacgggcggt tgctgcattc ggagatggtg
     2281 ccggcaggtc cgttctccat cacggatgtg ccgctgtaca ccagtgggga tgtgaccatg
     2341 aaaatcaccg gcgaggacgg tcgtgagcag actcagctct tccctttgtc ggtgatggcc
     2401 gggcagttaa acccgggaca acacgagttc agcgtggcag tcggtttgtc tgacgatgac
     2461 agtgacctga aaggcggcgt gtttgcggcg tcatacggtt acggtctgga tggactgacg
     2521 ctgcgcaccg ggggcgtgtt taaccgggac tggcagggta ttagcgccgg ggctgttctg
     2581 gggctgagtt atctgggggc agtgtctgct gacggggctt atgccatggc ggaataccgt
     2641 gacggcagcc gcagcggaaa taaggtgcag ttgtcctgga gtaaacaact ggagatgacg
     2701 aacaccggac tgcgggtgag ctggtcacgg cagagtgagg aatatgagga catgtcctcc
     2761 tttgacccga cagagctgtg gtcgcagtca aatcatggtc gccggccgaa ggatgaatgg
     2821 aatgccggta tcagtcagtc ggtggggggg ctgttcagtc tgtcggtgtc cggctggcag
     2881 cggagttatt acccggcaca gatgactggc cgttatcggt acagcgatga caatggtctg
     2941 gacacaggga ttaccggtac cctaagcaca cagattaagg gcgtcagtct gaatctgggc
     3001 tggtccggct cacggaattc gcagggagag aataacaggt cggcgtcagc ctctatctcg
     3061 gtgccattca cgctgtttga gcgtcgctac agcagcagta catcggtgag taccagtaaa
     3121 ggcggtggca cgggcttcag cactggcgtg tccggctcgc tgaatgaccg tttcagctat
     3181 agcctgggcg gcggtcgtga cagcggtggt ggtgtgagta gttatctaaa cgcgtcgtac
     3241 agtggtgacc gggcttatct gaatggtgcc ctgaaccact cacagtccgg cggaaccagt
     3301 ggttctgtat cagtcagcgg ttcggtactg gtcgtcccga tggcgaaaga cattatgttc
     3361 agccgcacga ccggggacac cgtggccgtg gtgaacgtga aggatacacc gggtgtgaag
     3421 gtgacgtccg gtgacggaca gactgacagt gacggcaacc tggtggtacc gctgaacagc
     3481 tatgactgga acacggtgac gattgatgcg ggcacactgc cgctgagcac cgaactgacg
     3541 aacaccagtc agaaggtggt gccgacggac aaggcggtgg tctggatgcc gtttgatgcc
     3601 ctgaaagtta agcgttacct gctgcaggtg aagcagcgtg acggtgagtt tgtgccaggg
     3661 ggaacctggg cacgtgacag taagaacaca ccgctgggct ttgtagctaa caatggtgtg
     3721 ctgatgatta acacggtgga tgcgccgggt gatatcaccc taggccagtg ccggatacct
     3781 gcggccagac tgcaggatac tgagaaacta caggagatta cgtgtgagta agcgtaacgc
     3841 agtaacgacg tttttcacta accaggtgac aaaagcactg ggaatgacgc tggcgctgat
     3901 gatgagctgt cagagtgcgg tggcttccct ggcggcagac cagacccgct atatctttcg
     3961 cggggacaag gatgcgctga ccatcacggt caccaacaat gacaaggaac gtacctttgg
     4021 tggtcaggca tgggtggaca atatcgtgga gaaagacacc cgtccgactt ttgtggtgac
     4081 gccgtccttc ttcaaggtga agccgaatgg tcagcagaca ctgcgtatca tcatggcctc
     4141 ggaccatctg ccgaaggata aagagtcggt gtactggctg aacctgcagg atattccgcc
     4201 ggctctggag ggcagcggta ttgcagtggc gctgcgcacg aaactgaaac tgttctatcg
     4261 cccgaaggca ctgcttgaag gtcgcaaggg ggcagaagaa ggcatcagtc tgcagagccg
     4321 cccggatggc aggaccatgc tggtgaacac cacgccatac atttttgcga ttggcagcct
     4381 gctggacgga aacggaaaga aaattgccgc ggataacgag acggcgcaga aactgctgat
     4441 gttcatgccg ggtgatgaag tacaggtgaa gggaaatgtg gtgaaagtgg actctctgaa
     4501 tgattacggt gaactgcaga catggacgat taaccagaag aaatcagcaa tatcgtctgc
     4561 acagaagtca tctgataaaa aataatcgcc ctccggcata cggaacagag gggtaataaa
     4621 tgaagaaaac aatgatggta gtcgccttgg ccctgagtgc actcagtatt cagtcaacac
     4681 ttgccgctga atacagcgag aaagcgcagt atctgggggt ggtgaatggc caggtggtgg
     4741 gtaacagcgt ggtgaaggta acccgcatac cgacagatcc ggtgctgtac cggagtggag
     4801 acacgacgcc tcttcccgac cgcctgacca tccgtaatgc agaatccaga gcagcatctg
     4861 gtggcttggc gtatatcacg gtgaaacagg tgctgccgga taacggggaa gcccgcatca
     4921 ccctgaagac ggccctgatg gttgacggaa agaaagtggc aatcagcgcc agacagcagg
     4981 gtgaggatat ggtgataact ttgccggaag cacagaagca gattgaatta agaacggatg
     5041 caccggcaga gctggaagta ccggtcagtt accggggaaa tctgcagata gcgctgcagg
     5101 tggaggactg aggattcatt tcctttataa atgcagtaca tcccgcgata ccggagcggg
     5161 tcatattggt aaagggattt accgtctttt aaaacgggaa taacgtaaaa ctattctgag
     5221 ttaaacactg tgtttaatga aatgcggtta tttaaatgaa tccgcagggg atggttttgc
     5281 ggtaattccg ggaaaataag gattaccgat tcagtttatt atttatgaaa cgtcaaggtg
     5341 attattttat gaaaaagact ctgattgcac tgactgtggc tgcttctgcc gcagtatccg
     5401 gctctgctat ggcgtggaat ccctctggcc ttggtggcaa cggtggcacc tttagcatga
     5461 gtggtactct gagcaaaact gagaaaacag taccgtgggc agtggaaata ggtgctccgg
     5521 taacagcgct tgatgtggcc gttaaacctg accagaagaa tgttttcatt cttttagaaa
     5581 aagacatacc gatacttggc attcgtagca acccgactgg tttcatgggg gaaaagggta
     5641 ttgccccaca aatcgattat aataaagccg ctgatgtgga ttatatggat aaggggaatt
     5701 tacctgcgca agttaatgtc acatcatctg atggtaagaa aattggtact ctggcgttcg
     5761 taatgcgagt tgcctgtcag gccggcgaaa agagggttga aagcgacgac aagttagaga
     5821 gtaacatgct attcgccagt tcaaaaggta aagctttctt tggtggagtt gccaaagatg
     5881 acaaaggtgt ctggagtgac gccgctgcct atacttgggc tacccacctt tttagcagta
     5941 ttgctaactc gtgggatact aaaattcctt acacggcggg cgatacagat gaaaccgact
     6001 tcagtaatgg agacaagctt taccgaggtt actatgcatc cggccttgcc aaggatatcc
     6061 ccatggaact agttcttgat cagactataa cgtctgctac cccagttccg tggaaagctt
     6121 ctctgccaat tgttatctcc tacatgtaat tcagcagtgt tcagcagtaa gcacttaccc
     6181 gtttactgct gattaaaact tcagaacaca gtttccggtg acccgacctg aatatacaga
     6241 accggtatgt cagaacaggt gttttttctg ttggagtgtc ttgtgacgca tattggttct
     6301 gtgctttttt taacaaaatt attttattga gctgttttgt tttatccgga taaacaggaa
     6361 tgaatgccga tgaaaataac gcatcattat aaattcttcc tttctgctgt tattttggta
     6421 acaatatttt attcggcagt gccatatgca aatattcttg atggcggcga aattcagttt
     6481 aatggctatg ttactgatga agcacccaaa tgggcctggc agataagttc tcccgaccag
     6541 acctggtctg tggatactgc cgatgcccgt acagagaacg ggcaactggt ttttaatttg
     6601 catgataaag gggctctgcc atttctggag ggacatctgc atgatgtggc tgagcgcggt
     6661 ggtcccggat tcacgccatt tattactttc agcagtaacg gacagccttt tactgtgacg
     6721 gaaggaaatg gtgcctcagc acagcacttt cgcgcctctg tcccggtgcg tgacccggag
     6781 acggggaacg tgtcaggaca gctttttttt accctgaatc agggaatggc ggtcagtacc
     6841 ggcagacagg acgacggggc atccgtaccg gctggcatgt cgctggtcag tggacagagt
     6901 gtgacggatg tacagccggg aagtctgcca cagggtctga aagcccgact ctcttccctg
     6961 ttactgatga atcagaattt tggcaatggc atgaatgcgg tggataacgg acaggttatc
     7021 aaccagggga tactgactga cggtcgggtg atggatctgg ctgcggcata tgcctctgag
     7081 gtgtcggatt ttgaactgcg gttgccggca gagggtacac ctgcagcatg gcaggcgggg
     7141 ctgaatgtga cggttacagt acagtgatgg ttgcagggat ataaggagag aagagaatga
     7201 aaaaggtgac gttgtttctg tttgctgcca gccacctgcc ttcctgtgta ctggcctgga
     7261 acacgccagg agaaaatttc agcggagagc ttaagctgga aggcccggtg accagtaccc
     7321 gtaacccgtg ggtatggaaa gtcgggcagg gaaatggagg tctggaggtt aaacagagtg
     7381 gcggtggggg ggggattcag gtcgcactgc cggcgatgac cgttttactg ggaaaaacca
     7441 ccctgaccac accggcagga cgtgaggggc tttcaccccg ggtcagttac ggaaaaggca
     7501 ctgaggggtt ttcacttgta tggacagcgc ctggcatggc tgaagtgacg ctacctgtca
     7561 ccggagataa taatgcccgg gcaggcacat ttgttttcag aattcaggct gtgggggtgt
     7621 tgcgccatgt gcagggcgga caaccggtgt atgttggtat gtatgacgac ctgaatacga
     7681 atggtttgcc aggtgaaagc actgccatga aggtttctga tattccgggc gttctgcaga
     7741 agatgttcag tggggaaggc cccggctggc ttcagacaat gacactcagt ggttattcag
     7801 gggtgagtca tttcagtgat gacttcctgc gccaggttga aggcgtgtat ggtgcacaga
     7861 ctgtggctgg cagtggtgaa ttacggctga acggcgagat gccggaacac tggagggtgt
     7921 cactgtcagt aaacattgaa tatcagtaag gagcaggagt gcgttttata atccggagat
     7981 aaggaacgtc ggttactgta agattgggga gttgtgttga tagcgggagt ggctggaata
     8041 agtggtatac atagataaaa attaaaacta tcactatttg agtgagtttt ttcctgctct
     8101 ccatgggctt tatagctcac aaaaaataga ataatattat taattattct gcttttttat
     8161 gggcattccc ccttgaaaaa tgagtttgtg ccagaatatt attaatatgt tttttgtgat
     8221 ttactaatca aggattgctg aatagcatcc agtactgaat attttgttca attatgtcag
     8281 gaaccatacc gaaaatcaaa acaggtgaaa atgttctgtc tgcagccatt cagcgcattg
     8341 aatggctatt tggaacgttt ccttctgtct gcttgtcctt ttctggtggt aaggattcta
     8401 ccgtgctttt ccatcttgtg tccgaagtgg cccgcaaaaa gaggcgtcgt ttttcggtat
     8461 tgttcattga ctgggaagca caatatcaat gcaccattac gcatattcag aagatgcaga
     8521 agatgtacct ggatgtgacg gacaggtttt actgggtggc ccttcccctg actacggtaa
     8581 acggtgtctc tcagtttcag ccggagtgga tatgctggga gcctggtgtg acctgggttc
     8641 gccatccacc ggaatatgct attacggata tggcgtattt tcccttttac cgatatgcca
     8701 tgacctttga ggaatttatc ccggcgtttt cctcctggtt tgccagggat aaatacctgg
     8761 tggctgttct gaccggagtc cgggcagatg agtccctcaa ccgctttatg ggactggtat
     8821 cccgtcgcaa acagagatat gccgatgata aaccctggac tacggcatca cctgaagggt
     8881 tttattacac catgtatccg ttgtatgact ggaaagcccg tgatatctgg atatataacg
     8941 ccagaacccg agctatctac aatcccctgt atgacctgat gcaccgtgcc tgtgtgccgt
     9001 tacgcaacat gcgtgtctgt gagccttttg gtcctgaaca gcgtaagggg ctgtggcttt
     9061 atcatgttct ggagccggaa acctgggcca ggatgtgtga gcgggtgtcg ggtgctgcca
     9121 gcggtgcgct ttatgccaat gagagcggtg cctattttgc cctgcgtaag cgtatcacaa
     9181 agccgcctca tcatacctgg cgtagctatg cgatgttcct gctggatgtg atgccggaaa
     9241 gaacggcaga acattaccgt aataaaattg ctgtctacct gcgctggtat cagacgcggg
     9301 gcttcccgga tgacatcccg gatgaacagg agaatgacct ggggagccgg gatatcccgt
     9361 cctggcgacg tatctgtaag acactcataa agaatgattt ctggtgccgg actctctcct
     9421 tcagtccgaa caaaccccgg cactatgaac gttacctgca gcgtatgaag gaaaggagga
     9481 aggaatgggg gattctgtga caccggagat acttggcaac atgattcgtc agtatttcag
     9541 tcaggtacga actgaaaaag agagcataca ggctctgaat catctgcgca gagtgcttca
     9601 tgaggtcagt ccttttgcac aggagcctgt ggactgtgtg ttgtgggtga aagcggatga
     9661 ggtggtggcc aacgattaca accctaatgt catggcacta ggtgagaaaa aactactgaa
     9721 acagtctctg gagaaagatg gtttcacaca accggtggtg gtgtcagagg agaagaatca
     9781 ttatctggtg gtggatggtt ttcatcgtca gttactgggg cgggaagcag atacaggaaa
     9841 acgcctgagg ggctggttgc ctgtagcctg tattaatccg gagagaaagg gacaggccgc
     9901 acgtattgcc gcgaccattc gacataaccg ggccaggggg aaacaccaga tcactttaat
     9961 gtctgatatt gtcagggatc tttcacgtct gggctggacg aatgaacgta ttggtacaga
    10021 gctgggaatg gaccaggatg aagtgctgag gctgaaacag attagcgggc tgacagagtt
    10081 gtttcaggaa gaggatttca gctcggcttg gacggttcgt tgatgttgtt aaatatgggc
    10141 ggtatttctg aaagttgata ctttgggtag aaaaaatgat accgaaaact atggaggatg
    10201 tgcttgtcag gtgactggac accataggtc ttaggctttg ataacgggat aagatgagct
    10261 gcttttctca taccatcagg tgtgatgatg gcgcagattt acaaaatctg attgattcta
    10321 aagatgtata agtaaaaatc aagctttgat atgaaaaaac agatatttat taataataaa
    10381 cctcctgtag tcccttactc agggacgcac gcgaaaatat ttaaatacat tgaaatccct
    10441 ctgccgtttt tctactttat ctatacatca ggcgaaccat tccacatatc tgtccaaaat
    10501 actgttattt atgtatcaaa atacaatggt atatttatta ataaattagt tcctttttct
    10561 cttttatttg acagggatat atctgttctt caaagaagag atatttgtgt tgtgcgattt
    10621 acgtcagagg aaataagtga acataacgtt ttatttgatc atgacattga acgattaaaa
    10681 aaaatttcca aagctcagct tatatctccc gattatgttt tgattgattt ttctagtggg
    10741 gggggagatg aaccctatgc aatgtcctgg ttaatatctc agtgtgctca tcagtgcact
    10801 gataataaaa agacggagac agatgccatt tacgataaag ttcggagttc ttatttgctt
    10861 tcatgtattc tgaaaaaaaa caaaaatgtt gggcttattc ttcatgcacc atcatttgtt
    10921 tctgtgtctg aaaaaattgc cagaattgtg atggcaaatt actcccgtaa ctggagtaat
    10981 tccgagttgg ccagtgcggt tttaatgagt gaatcttctt taaaaagaag aatgtacaaa
    11041 gaggtcggtt ctataagtac atttgtacat aaaataaagc ttacagaagc tatcaggaaa
    11101 ttacgaagga caaatactcc catttctgtc atatcatctg agttaggtta cagttcacca
    11161 tcgtattttt ctaaagtttt ctttaaatat ttaaagacat accctcaaaa cataaggaag
    11221 aaaaatggtc ggtaatgtca ccaattagga ggacttgcag agccgctttt ttacgatact
    11281 caccccgtaa gtaagcgata attggtaact taaacgccca tctgacatag atggacacct
    11341 gagtatggaa ttacaggact ggcgaaaaga acctcgtaaa aagtatttgg tgcaaatcgt
    11401 tggttccttt gctgaatttg aacgcgccat gcttcgtgaa cggacaaaga gtggacttgc
    11461 agcggcacgc caggacgggt tggtggatgg cgactaaagt taacacccca gcaacaaaaa
    11521 gagattgtct cgctcgtcac atcggggcag aaaacaggtg ctgatgccgc ccgtctgttt
    11581 cgggttcacc cttccaccgt ggtgcgtctt ctcgccagac accggatgac tgagatcaaa
    11641 cagacataat ctatgcggag atttacagca gggcatggtt cacaaatctg acagcgatga
    11701 attatcggca ctgtgggcag aaaatgctcg
//
LOCUS       DBKEKI010000122        11571 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.122, whole genome shotgun sequence.
ACCESSION   DBKEKI010000122 DBKEKI010000000
VERSION     DBKEKI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.122"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(127..894)
                     /gene="hdhA"
                     /locus_tag="ACUQPX_003266"
     CDS             complement(127..894)
                     /gene="hdhA"
                     /locus_tag="ACUQPX_003266"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HHV9057869.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(1006..2034)
                     /gene="malI"
                     /locus_tag="ACUQPX_003267"
     CDS             complement(1006..2034)
                     /gene="malI"
                     /locus_tag="ACUQPX_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HHV9057870.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPATLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            2209..3801
                     /gene="malX"
                     /locus_tag="ACUQPX_003268"
     CDS             2209..3801
                     /gene="malX"
                     /locus_tag="ACUQPX_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HHV9057871.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPAALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3811..4983
                     /gene="malY"
                     /locus_tag="ACUQPX_003269"
     CDS             3811..4983
                     /gene="malY"
                     /locus_tag="ACUQPX_003269"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HHV9057872.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            5087..6088
                     /gene="add"
                     /locus_tag="ACUQPX_003270"
     CDS             5087..6088
                     /gene="add"
                     /locus_tag="ACUQPX_003270"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HHV9057873.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(6124..7128)
                     /locus_tag="ACUQPX_003271"
     CDS             complement(6124..7128)
                     /locus_tag="ACUQPX_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HHV9057874.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEDWGYDMRDGVLTRVEGEERV
                     EETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGIESAKHRATLCLA
                     "
     gene            7371..7496
                     /gene="blr"
                     /locus_tag="ACUQPX_003272"
     CDS             7371..7496
                     /gene="blr"
                     /locus_tag="ACUQPX_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HHV9057875.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7769..7984
                     /gene="ydgT"
                     /locus_tag="ACUQPX_003273"
     CDS             7769..7984
                     /gene="ydgT"
                     /locus_tag="ACUQPX_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HHV9057876.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            8070..8510
                     /locus_tag="ACUQPX_003274"
     CDS             8070..8510
                     /locus_tag="ACUQPX_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HHV9057877.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8587..9168
                     /gene="rsxA"
                     /locus_tag="ACUQPX_003275"
     CDS             8587..9168
                     /gene="rsxA"
                     /locus_tag="ACUQPX_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HHV9057878.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            9168..9746
                     /gene="rsxB"
                     /locus_tag="ACUQPX_003276"
     CDS             9168..9746
                     /gene="rsxB"
                     /locus_tag="ACUQPX_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HHV9057879.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDESNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9739..>11571
                     /gene="rsxC"
                     /locus_tag="ACUQPX_003277"
     CDS             9739..>11571
                     /gene="rsxC"
                     /locus_tag="ACUQPX_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HHV9057880.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIAHPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KAHKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAE"
BASE COUNT         2697 a         2988 c         2919 g         2967 t
ORIGIN      
        1 ttcacccagc aattggttac ccaatagccg tgatttggct gccagttgtt tagcagagac
       61 gttgatgaat tgcacacgct atcccggtaa acaacacata cgaccgttta ccggttcgtt
      121 agtgtattaa ttgagctcct gtaccccacc accggagacg gtgagaattt gtccgcttac
      181 ccagctggct gcaggcgagc aaaggaacag cgccgcgtta gcaatatctt gcggttggcc
      241 cagacgtctg atcggcgtgt gctgtaacat tttttgttca atttctggtg taataacgga
      301 tttcagggca tcggttaata ttgcccccgg cgcaatgcca ttcacccgaa tatttttttc
      361 acccaggtca aatgccatat ttctgaccag atgactggcc gcagctttag atgatgcata
      421 ggaagtcatg tttatatttt tattttctgc cgccatagaa gtgatggtca gaataacgcc
      481 accgccattt ttttccattt ctggcgcaac aagttgtgac agatggaaaa aagaaaacac
      541 attcagttca taagcacggc gaaaatctgc cattggcata tcaaacggtt taggaccacc
      601 gccaccggcg ttgttaacca ggatatcaac tttacccagt ttactgacgg caaagtctgc
      661 cagtgcagag agttcctgtt cggaagtaat atcacaacgc caggcaaatg cctgaccacc
      721 cagttgttga atttcgtcta caacatggtt agctgcgtcg gcgttaatat cactgaccac
      781 cacagatgcg ccagctgtcg cgaatgtaat ggcgatttct ttaccaatac ctgcacccgc
      841 acctgtgatg atggcgcatt ttccgtcgag tctcaggttg tcagaattaa acacataaac
      901 ctcctgtgaa cttcagtaaa aataaaaggc accgcctgtg tgccacattt tttatcatta
      961 accttgagga aaggacacta ccaagtatag cagataaaga gaggattatt tcgccgcaat
     1021 gagccgggcg ggaataataa gattgcgtga atgcgtctct tcatgggtga ttttttgcat
     1081 catgcgatcc gcaagtgtga taccaagttc ccgcgctggc gtgctggccc aggtaacagg
     1141 tatatcatca agtgtggctg gtgtcgcatc ggtaaatgcc gccagcgaaa cctgttgctc
     1201 aaagtaacga tcgactccgc tttccccgct ttgccgccct gctttcagca aaccaaacca
     1261 tgcccccatc gcaatagttt cgttatagca aaccacggca ctgatggtcg ggttatgacg
     1321 taaaagcgcc gtgatagctt ccgcggcttg cttctggctg gaagtgcact ccaacaccca
     1381 atcgctgtga aacggcaggc caaattttag tagagttgca caatagcccc ccacccgttc
     1441 tgcacgggtt aatgaggaac tttgccctcc cagccaggcg atccgctgat gcccattgcg
     1501 aatgagatgc tccgtcaaca actgtgcagc ctgcatgttg tccgggcgaa ccgtatcaac
     1561 atcatcaaga taactggcac gggaagcgaa aatcaccggg atagcttttt cttctgccat
     1621 ccgtcgcagg tcatcgctgc ttcctgcagc cccggcaatt accacaccat cgacaccctg
     1681 attcagtaac agtgaaaacc gctgtgccag ctgctcgccg tctttaccgc cgtgaagcaa
     1741 aaaaaccatc cgtccctgcg cttccagagc ttccgtcaat ccggccgtca attcggcgta
     1801 aaacggcgca gataaatcac ggacgatcaa accaatgacg ccgctttgcc cgccgcgcag
     1861 cgccgacgcc tggcgattgc gcacaaatcc cagctcttca atggcggcgt taacgcgttc
     1921 tcctgtggcg gtagagattc gccctttgcc actaagcacc agcgaaacgg tacttaccga
     1981 cacgcccgca gccagcgcaa catcatgaat ggttattttt ttggcggtag ccatgcagat
     2041 gacctactcc ctgattatgt gacagataaa acgttttacc ttttatttat cttatacccg
     2101 ctattatcgt tgcgtaatgt gatttatgac tcactaaaat ttgataaaac gttttatctt
     2161 ctcgcgcaat ttactgaatc cagattgttc tctacgagga gtcgttttat gacggcgaaa
     2221 acagcaccga aagtcacgct gtgggagttc ttccagcagt taggcaaaac cttcatgtta
     2281 cccgtggcat tattgtcgtt ctgcggcatt atgctcggca ttggtagttc tcttagcagc
     2341 catgatgtca taaccctgat cccggtcctg ggcaaccccg tgttgcaggc tatctttacc
     2401 tggatgagta agattggctc gtttgctttt agtttcctgc ctgtcatgtt ctgtatcgcc
     2461 atcccgctgg gcctggcacg cgaaaataaa ggcgtagcgg cattcgctgg cttcgtcggt
     2521 tatgcggtaa tgaacctcgc ggtaaacttc tggttgacca ataaaggcat tctgccaacc
     2581 acagatgccg ccgttctgaa agccaataac atccagagca ttcttgggat ccagtcgatc
     2641 gataccggga tcctcggtgc ggtgatcgcc ggtattatcg tctggatgct gcatgagcgt
     2701 ttccataata tccgcctgcc ggctgcgctg gcattcttcg gcggtacgcg cttcgtacca
     2761 attatctcct cgctggtgat gggccttgtc ggcctggtga ttccattagt ctggccgatt
     2821 ttcgccatgg gtattagcgg cttgggccat atgataaaca gcgcgggtga tttcggaccg
     2881 atgctgtttg gtaccggtga acgtctgctg ttgccgtttg gtctgcatca cattctggtg
     2941 gcattaattc gctttaccga cgcaggcggc acgcaggaag tctgcggtca aaccgtcagc
     3001 ggcgcactga ccatcttcca ggcgcaattg agttgcccga ccactcacgg tttttctgaa
     3061 agcgtcacgc gtttcctttc gcaaggtaaa atgcctgcgt ttctcggcgg tctgccaggt
     3121 gcagcgttag ctatgtatca ctgcgcgcgc ccggaaaatc gccataaaat taaaggtctg
     3181 ctgatttctg gcctgatcgc ctgcgtcgtt ggcggcacta ccgaaccgct ggaattcctg
     3241 ttcctgttcg tagcgccagt tctgtatgtc atccacgcgc tgttaaccgg cctcggcttc
     3301 accgtcatgt ctgtgctcgg cgtcaccatc ggtaataccg acggcaatat catcgacttc
     3361 gtggtgttcg gtattttgca tggtctgtca accaagtggt acatggtgcc agtggtggcg
     3421 gcaatctggt ttgtcgttta ctacgtcatc ttccgtttcg ctatcacccg cttcaatctg
     3481 aaaaccccgg ggcgcgatag cgaagttgcc agctcaatcg aaaaagccgt tgccggtgcg
     3541 ccgggtaaat caggttacaa cgttcctgca atcctcgaag cattaggcgg tgccgacaat
     3601 attgtcagcc tcgataactg cattacccgt ctgcgtttgt ctgtggaaga tatgtcgctt
     3661 gttaatgtgc aggcactgaa ggacaatcgg gcaattggcg tagtacaact taatcaacat
     3721 aacctgcagg ttgttatcgg gccacaagtt cagtcagtaa aagatgaaat ggccggtctg
     3781 atgcatactg tccaggcata aggataagat atgttcgatt tttcaaaggt cgtggatcgt
     3841 catggcacat ggtgtacaca gtgggattat gtcgctgacc gtttcggcac tgctgacctg
     3901 ttaccgttca cgatttcaga catggatttt gccactgccc cctgcattat cgaggcgctg
     3961 aatcagcgcc tgatgcacgg cgtatttggc tacagccgct ggaaaaacga tgagtttctc
     4021 gcggctattg cccactggtt ttccacccag cattacaccg caattgatac gcagacggtg
     4081 gtgtatggcc cttctgtcat ctatatggtt tcagaactga ttcgtcagtg gtctgaaaca
     4141 ggtgaaggcg tggtgatcca cacgcccgcc tatgacgcat tttacaaggc cattgaaggt
     4201 aaccagcgca cagtaatgcc cgttgcttta gagaagcagg ctgacggctg gttttgcgat
     4261 atgggcaagc tggaagccgt tttggcgaaa ccagaatgta aaattatgct cctgtgtagc
     4321 ccacagaatc ctaccgggaa agtgtggacg tgcgatgagc tggagatcat ggctgacctg
     4381 tgcgagcgtc atgatgtgcg ggttatttcc gatgaaatcc atatggatat ggtctggggc
     4441 gagcagccgc atattccctg gagtaatgtg gcgcggggag actgggcgtt gctgacgtcg
     4501 ggatcgaaaa gtttcaatat tcccgcgctg accggtgctt acgggattat agaaaatagc
     4561 agcagccgtg atgcctattt atcggcactg aaaggacgtg atgggctttc ttccccttcg
     4621 gtactggcgt taactgccca tatcgccgcc tatcagcaag gtgcaccgtg gctggatgcc
     4681 ttacgcgtct atctgaaaga taacctgacg tatatctcag ataaaatgaa cgccgcgttt
     4741 cctgaactca actggcagat cccgcaatcc acttatctgg cctggcttga tctacgtccg
     4801 ttgaatattg acgacaacgc gttgcaaaaa gcgcttatcg agcaagaaaa agtcgcgatc
     4861 atgccggggt atacctatgg tgaagaaggt cgtggttttg tccgtctcaa tgccggctgc
     4921 ccacgttcga aactggaaaa aggtgtggct ggattaataa acgccatccg cgctgttcgc
     4981 taaccccaat tgcgcaacgt aaaaaatcgt tgcgcaatcg tggattttta ccctgctttg
     5041 tttttataat ggtgcgcact tttatatcca gaaaaagagt gcgaccatga ttgataccac
     5101 cctgccatta actgatatcc atcgccacct tgatggcaac attcgtcccc agaccattct
     5161 tgaacttggc cgccagtata atatctcgct tcctgcacaa tcccttgaaa cactgattcc
     5221 ccacgttcag gtcattgcca acgaacccga tctggtgagc tttctgacca aacttgactg
     5281 gggcgttaaa gttctcgcct ctcttgatgc ctgccgccgc gtggcatttg aaaacattga
     5341 agatgcagcc cgtaacggcc tgcactatgt cgagctgcgt ttttcaccag gctacatggc
     5401 aatggcacat aagctgcctg tagcgggtgt tgtcgaagcg gtgatcgatg gcgtacgtga
     5461 aggttgccgc acctttggtg tgcaggcgaa gcttatcggc attatgagcc gaaccttcgg
     5521 cgaagccgcc tgtcagcaag agctggaggc ctttttagcc caccgtgacc agattaccgc
     5581 acttgattta gctggtgatg aacttggatt cccgggaagt ctgttccttt ctcacttcaa
     5641 ccgcgcgcgt gatgcgggct ggcatattac cgtccatgca ggcgaagctg ccgggccgga
     5701 aagcatctgg caggcgattc gtgaactggg tgcggagcgt attggacatg gcgtaaaagc
     5761 cattgaagat cgggcgctga tggattttct cgccgagcaa caaattggta ttgaatcctg
     5821 tctgacctcc aatattcaga ccagcaccgt agcagagctg gctgcacatc cgctgaaaac
     5881 gttccttgag catggcattc gtgccagcat taacactgac gatcccggcg tacagggagt
     5941 ggatatcatt cacgaatata ccgttgccgc gccagctgct gggttatccc gtgagcaaat
     6001 ccgccaggca cagattaatg gtctggaaat ggctttcctc agcgcagagg aaaaacgcgc
     6061 actgcgagaa aaagtcgccg cgaagtaaca aaaatggatg gtgcaaattg caccatccat
     6121 ttttcacgca aggcacaacg tcgcgcgatg tttggcggat tcgatgccca gctcaatcaa
     6181 ctccattacc tggattgcct ggcttgccgg aaccggattt tcaccatcgc catttaacgc
     6241 atcacgaatc gccgcatagt aagccggata attcccaggc accgtcaaca gcgtttcttc
     6301 gacacgttcc tcaccttcca cgcgggtaag tacgccatca cgcatatcgt agccccagtc
     6361 ttcctgtgga tcgaggccat atttcacata actgcctcgg gatccatgca cgatataccg
     6421 tgctgactca gcggctgcca gcatggtacc gtgtaaaatg actcgccgct gtggatagga
     6481 caagatggcg tggaaataat cggtcgactg cgctccgggc cgtaactgtg ccaaatctac
     6541 cgtcatgctg accggtaaac caaatagcgt aatggcctga tcaagaagat gcggtgctaa
     6601 atcgtaccag ataccgctgc ctgggccgcc ctgttcacgc caacgatcgc gcacctgcgg
     6661 acggaagcgg tcaaaatgag actcaaagta agcaacttca cccagcacgc cttccgcgag
     6721 caaacctttt agcgtcaaga aatcgctatc ccaacgacgg ttatggaata cagacagcac
     6781 acgccccagg ctttttgcca gcgcatccag ctctcgcgct tgtgacagtg tcacggtaaa
     6841 gggtttatca acgaccacat gtttacccgc ctcaagcgcc gctttggcta acgggaaatg
     6901 ggtatcgttg ggtgtaggaa tgacaatcag gtctatgttg ggatcgttaa acagatgctt
     6961 cggctcagag acaaccgtaa ccgttggcca gtcggctttt acttttgttt catcactgct
     7021 ggagattacc gccagttcca gcccgggcgt gcccgcaatc aggggcgcat ggaaggtttt
     7081 gctcgcataa ccatacccaa tcaacccaac acggatgttg tcgctcatgt cattgcctct
     7141 cattgaagta tgatggctat ttgacactat cctttaccca cgctcaacag tttaataacc
     7201 tgccagcaat aagggatgtt gtttaactta agtcaaaaaa atagcgaatt ttccaacgac
     7261 aaaagctaaa tatcgcaaaa acctcagtaa aaatcttgct ggagctatta ttgctaagta
     7321 acatttaccc cctgaagtta atggatcaat caagagagat gtgggctgta atgaatcgtc
     7381 ttattgaatt aacaggttgg atcgttcttg tcgtttcagt cattcttctt ggcgtggcga
     7441 gtcacattga caactatcag ccacctgaac agagtgcttc ggtacaacac aagtaagctc
     7501 tgcacttgtg gagcgacatg ctgcccgtcc gggtgcatgt tttcacttgt cggatattaa
     7561 accaggaatt tattatcttg ttcgatgttg ttggtgattg tcagggatag taaagcgcgt
     7621 tactcttttc agagcagttt ttcatccctg aatatttgcc atcagcgcat tcttattatt
     7681 gtaataacac aagcgttcac ttttggtgaa attttatcat cactaataat acctacccct
     7741 ttggtattaa ttattttatg gaccttttat gactgttcag gactacttat taaaatttcg
     7801 caaaatcagt tcactcgaaa gtctggaaaa actctacgac catcttaatt acaccctgac
     7861 ggacgatcag gaactgatca atatgtatcg tgctgccgat caccgtcgcg cagagctggt
     7921 ttctggcggg cgtttgtttg acctcggcca ggtaccgaag tccgtctggc actatgtcca
     7981 ataaagaaag tagcgatcgg ttataaaagc cttataataa cgcccctaaa attagatttg
     8041 cctgtttgcg tcgctattct ggagaaccta tgaccaccac aacgccacaa cgtattggag
     8101 gctggttgct tggcccgttg gcctggcttt tagtcgctct gttgagtacg acgctggcgt
     8161 tgttgctgta taccgctgcg ttatcttctc cgcaaacatt tcaaacgctc ggcggacaag
     8221 cactgacgac acaaatctta tggggcgttt cttttattac cgctatcgcc atgtggtatt
     8281 acacactgtg gttgaccatc gctttcttta aacgtcggcg ttgtgttcct aaacactata
     8341 ttatctggct gcttatttcc gtattgctgg cagtaaaagc attcgctttt tcacccgtgg
     8401 aagttggcat agccgttcgc cagttgctgt ttactttgtt ggcaaccgca ttgatcgtcc
     8461 cctatttcaa acgctcgtcg cgggtaaaag cgacgtttgt gaatccgtaa taaccttaca
     8521 gttaacctgt tgtcgcctgc tctggattaa cagataatag gcggcttttt tatttcaggc
     8581 cgaaaaatga ctgactacct gttactgttt gtcggaactg tactggtcaa taactttgta
     8641 ctggtcaagt ttctcggtct ctgtccgttt atgggggttt ccaaaaaact ggaaaccgcg
     8701 atgggcatgg ggctggcaac aacgtttgtg atgacgctgg cgtctatttg cgcctggctt
     8761 atcgatacgt ggattttgat cccacttaat ctgatttacc tgcgcaccct ggcatttatt
     8821 ctggtgattg ctgtggtcgt gcagttcacc gagatggtgg tgcgcaaaac cagccctgtg
     8881 ctttaccgtc tgctggggat ttttttgccg cttatcacca ccaactgtgc agtgctcggc
     8941 gtggcgttgc tgaatatcaa tctcgggcac aatttcttgc agtcggcgct gtacggtttt
     9001 tccgccgctg tcggtttctc gctggtgatg gtgctcttcg ccgccatccg agaacgcctt
     9061 gctgtggctg atgtcccggc accttttcgc ggtaatgcca ttgcgttaat taccgcaggt
     9121 cttatgtctc tggcctttat gggctttagt ggtttggtga agttgtaatg aatgctatct
     9181 ggattgccgt tgccgccgtg agcctgctgg gcctggcgtt tggcgccatt ctgggttatg
     9241 cctcccgccg ttttgcggtg gaagacgatc cggtcgttga gaaaattgac gaaatcttac
     9301 cgcagagcca gtgtggtcag tgcggttatc ccggctgtcg cccctacgcg gaagccatca
     9361 gctgtaacgg tgaaaaaatc aaccgttgcg ccccaggtgg cgaagctgtg atgctaaaaa
     9421 ttgccgagtt gcttaatgtc gagccgcagc cgctggatgg cgaagcgcaa gagctaacgc
     9481 ctgcgcggat ggtggctgtt attgatgaaa gtaactgtat tggctgcact aaatgtattc
     9541 aggcttgtcc ggtagacgcc atcgttggcg ctacccgtgc catgcatacg gtaatgagtg
     9601 atctctgtac gggctgcaat ttatgtgttg atccgtgccc gacgcactgc atctcgttgc
     9661 aaccggtcgc agaaacacct gactcctgga aatgggatct gaacaccatt cccgtgcgta
     9721 tcattcccgt ggaacaccat gcttaagtta ttctctgcat tcagaaaaaa taaaatctgg
     9781 gatttcaacg gcggcatcca tccaccggag atgaaaaccc agtccaacgg tacccccctg
     9841 cgccaggtac cactggcgca gcgttttgtt attccactga aacagcatat tggcgctgaa
     9901 ggtgagttgt gcgttagcgt cggcgataaa gtattgcgcg gccagccgct tacccgtggt
     9961 cgcggcaaaa tgctgcctgt tcacgcgccc acctcgggta ccgttacggc tattgcgccc
    10021 cactctacgg ctcatccttc agctttagct gaattaagcg tgattattga tgccgatggt
    10081 gaagactgct ggatcccgcg cgacggctgg gtcgattatc gctctcgcag tcgcgaagag
    10141 ttaatcgagc gcatacatca gtttggtgtt gccgggctgg gcggtgccgg attcccgaca
    10201 ggcgttaaat tgcagggtgg tggagataag attgaaacgt tgattatcaa cgctgctgag
    10261 tgcgagccgt acattaccgc cgatgaccgt ttgatgcagg attgcgcggc tcaggtcgta
    10321 gagggtattc gcattcttgc gcatattctg cagccacgcg aaattcttat cggcattgaa
    10381 gataacaaac cgcaggcgat ttccatgctg cgcgcggtgc tggcggactc taacgatatt
    10441 tctctgcggg tgattccaac caaatatcct tctggcggtg ctaaacaatt aacctacatt
    10501 ctgaccggga agcaggttcc acatggcggg cgttcatccg atatcggcgt attaatgcaa
    10561 aacgtcggca ctgcttatgc agtgaaacgt gccgttattg atggcgagcc gattaccgag
    10621 cgtgttgtaa ccctgactgg cgaagcaatc gctcacccgg gcaacgtctg ggcacggctg
    10681 gggacgccag tgcgtcattt attgaatgat gccggattct gcccctctgc cgatcaaatg
    10741 gtgattatgg gtggcccgct aatgggcttt accttgccat ggctggatgt cccggtcgta
    10801 aagattacca actgtctgtt ggctccctct gccaatgaac ttggcgaacc acaggaagaa
    10861 caaagctgca tccggtgtag cgcctgtgct gacgcctgcc ctgtggatct tttgccgcaa
    10921 cagttgtact ggttcagcaa aggtcagcaa cacgataaag ctaccacgca taacattgcc
    10981 gactgcattg aatgtggggc ttgcgcgtgg gtttgcccga gcaatattcc cttggtgcaa
    11041 tatttccgtc aggaaaaagc tgaaattgcg gctattcgtc aggaagaaaa gcgcgccgca
    11101 gaagccaaag cgcgtttcga agcgcgccag gctcgtctgg agcgcgaaaa agcggctcgc
    11161 cttgaacgac ataagagcgc agccgttcaa cctgcagcca aagataaaga tgcgattgct
    11221 gccgctctgg cgcgggtgaa agagaaacag gcccaggcta cacagcctat tgtgattaaa
    11281 gcgggcgaac gcccggataa cagtgcaatt attgcagcac gggaagcccg taaagcgcaa
    11341 gccagagcga aacaggcaga actggagcaa actaacgacg cagcaaccgt tgctgaacca
    11401 cgtaaaactg ccgttgaagc agctatcgcc cgctccaaag cgcacaagct ggaacagcaa
    11461 caggctaatg cggaaccaga agaacaggtc gatccgcgca aagccgccgt cgaagccgct
    11521 attgcccgcg ccaaagcacg caagctggaa cagcaacagg ccaatgcgga g
//
LOCUS       DBKEKI010000123        11533 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.123, whole genome shotgun sequence.
ACCESSION   DBKEKI010000123 DBKEKI010000000
VERSION     DBKEKI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.123"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..727
                     /locus_tag="ACUQPX_003278"
     CDS             <1..727
                     /locus_tag="ACUQPX_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HHV9057881.1"
                     /translation="FASNNTGLSGPVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAP
                     VATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQ
                     KYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDT
                     DQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDK
                     QQGLSCTITFGKEIDESRNYICQ"
     gene            743..1776
                     /locus_tag="ACUQPX_003279"
                     /pseudo
     CDS             743..1776
                     /locus_tag="ACUQPX_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(1858..2196)
                     /gene="rcnB"
                     /locus_tag="ACUQPX_003280"
     CDS             complement(1858..2196)
                     /gene="rcnB"
                     /locus_tag="ACUQPX_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HHV9057882.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(2415..3263)
                     /gene="rcnA"
                     /locus_tag="ACUQPX_003281"
     CDS             complement(2415..3263)
                     /gene="rcnA"
                     /locus_tag="ACUQPX_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HHV9057883.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHDHEHHQDHEHHHDQGHHHHHEHGEYQDA
                     HARDHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLV
                     VSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHG
                     FMGIMR"
     gene            3385..3657
                     /gene="rcnR"
                     /locus_tag="ACUQPX_003282"
     CDS             3385..3657
                     /gene="rcnR"
                     /locus_tag="ACUQPX_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HHV9057884.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      3749..3845
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3880..4668
                     /gene="thiM"
                     /locus_tag="ACUQPX_003283"
     CDS             3880..4668
                     /gene="thiM"
                     /locus_tag="ACUQPX_003283"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HHV9057885.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            4665..5465
                     /gene="thiD"
                     /locus_tag="ACUQPX_003284"
     CDS             4665..5465
                     /gene="thiD"
                     /locus_tag="ACUQPX_003284"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HHV9057886.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            5530..6348
                     /locus_tag="ACUQPX_003285"
     CDS             5530..6348
                     /locus_tag="ACUQPX_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HHV9057887.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQWVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            6400..7146
                     /locus_tag="ACUQPX_003286"
     CDS             6400..7146
                     /locus_tag="ACUQPX_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHV9057888.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSHVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(7120..8085)
                     /locus_tag="ACUQPX_003287"
     CDS             complement(7120..8085)
                     /locus_tag="ACUQPX_003287"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HHV9057889.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(8082..9085)
                     /locus_tag="ACUQPX_003288"
                     /pseudo
     CDS             complement(8082..9085)
                     /locus_tag="ACUQPX_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(9082..10359)
                     /locus_tag="ACUQPX_003289"
     CDS             complement(9082..10359)
                     /locus_tag="ACUQPX_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HHV9057890.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPNTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10616..>11533
                     /gene="fbaB"
                     /locus_tag="ACUQPX_003290"
     CDS             10616..>11533
                     /gene="fbaB"
                     /locus_tag="ACUQPX_003290"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HHV9057891.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTA"
BASE COUNT         3034 a         2825 c         2925 g         2749 t
ORIGIN      
        1 ttttgcctca aataatacgg gattatcagg accagtaggg agtcgggatc agttcaatta
       61 tggtgtcaac ctgagtcatc aacatcaggg aaatgaaacg acagctgggg cgaatttgac
      121 ctggaacgcg ccggttgcga cagtgaatgg cagttatagt cagtcgagta cttatcgaca
      181 gactggagcc agtgtttcag ggggcattgt cgcctggtcg ggtggcgtta atctggcgaa
      241 ccgtctttcc gaaacgtttg ctgtgatgaa tgcgccagga attaaagatg cttatgtcaa
      301 tgggcaaaaa tatcgcacaa caaaccgtaa tggagtggtg gtatacgacg gaatgacacc
      361 ttatcgggaa aatcacctga tgctggatgt gtcgcaaagc gatagcgaag cagaattacg
      421 tggcaaccgg aaaattgccg ccccttatcg cggcgcggtt gtactggtta attttgatac
      481 cgatcagcgc aagccctggt ttataaaagc gttaagagcg gatggtcaac cattaacgtt
      541 tggttatgaa gtcaatgata tccatggtca taatattggc gttgtcggcc agggaagcca
      601 gttatttatt cgcaccaatg aaataccgcc atcggttaat gtagcaattg ataagcaaca
      661 aggactttca tgcacaatca ccttcggtaa agagattgat gaaagtagaa attatatttg
      721 ccagtaatag tcaggtggtt ctatggaaat tcgcataatg ctatttatat taatgatgat
      781 ggttatgcct gtgagctatg cggcatgtta tagtgagtta tctgttcagc acaatttggt
      841 tgttcagggg aattttgcac ttactcaaac acaaatagcg acatatgagc ataattttaa
      901 tgattcgtca tgcgtgagta caaatactat cacccctatg agcccatcgg atattattgt
      961 tggactttat aacgatacca ttaaattaaa tttacatttt gaatggacca ataaaaacaa
     1021 catcacgttg tcaaataatc agagcagttt caccagtggt tattcggtaa cggtgacacc
     1081 tgcggccagt aatgcaaaag tgaatatttc tgcggggggc ggtggttcag tgatgattaa
     1141 tggtgttgcg acattatcca gtgcttcatc atcgacacgc gggagtgccg cagtacaatt
     1201 tctactgtgt ttattaggtg gcaagtcatg ggatgcatgt gtaaatagct acagaaatgc
     1261 attggcacaa aatgcaggtg tctattcctt caatctgaca ttgtcataca acccgataac
     1321 cacaacctgc aaacaggacg atttattaat tactttagac agtattcccg tttcacaatt
     1381 accagccaca ggtaacaaag caacaataaa tagtaaaaaa agggatatta ttctgcgttg
     1441 taaaaattta ttaggtcaac aaaatcaaac atcacggaaa atgcaggtgt atttatcaag
     1501 ttctgacttg ttaaccaaca gcaacacaat actgaaaggt gcggaagata atgcgtagga
     1561 tttattcttg aaagtaatgg ttcgccagtc acacttttaa atatcactaa cagcagtaaa
     1621 ggatacacaa atttaaaaga aattgcggca aagtcaaaac ttacagatac aacggtttca
     1681 attccgataa cagccagtta ctacgtctac gatacaaaca aagttaaatc tggcgcactg
     1741 gaggcaaccg cattaatcaa cgtgaaatac gactaataac aagcaagacg agcaagtggc
     1801 taataataaa aaataacaag gtgtctgcat ttccccctca tgatgagggg gcttttttta
     1861 gcgatgataa aaaatctcac cgtcgtaggc tttaatgatt ttaccgtcgg tgtcgctgat
     1921 caacacgtac gcgccaccca tataggtcca gtgcgtcccg gcatcaggcg cgggcaggtt
     1981 acgtaactgc cactgcttaa tgttgtactc atcggtgcgg tacatctctg ggacggtatc
     2041 acctggtttg aaatgcttaa agtcagcaat gaaactactt aactcatact tagaaattcc
     2101 cgaggtattg gtcgaacccg cggtggctgg tgcggcccag gcggcactgg taaccagcaa
     2161 aagcacaccc agcaacatct tatttttaat ggtcataaac tctccacttt ggggatctgt
     2221 aatatcagtc gcatatcttt cctgccatac aatataactg acaagcgata ttttgttagt
     2281 gatcccatga tttcgtaaaa agtgtaaatg aaaggatgcc ctaaaagcat ccttagcgag
     2341 cagagagtca gcggaaatag ctgatttaag aatcatcctg ggctgaagat aacatgcaag
     2401 cctaaaggat tgagttatcg cattatgccc atgaagccat gtacgcccat atacacaccg
     2461 actaagccaa tcaacagact ggaaaaatag ggggcgcgtt tagcgagagt gttaaatccg
     2521 ctccagcgtt ttgcgacctg ctgaacgctg attgctgcgc caacgcctac ggtgacaagc
     2581 gttaacgcca ggccaatgct gaaactgacg accagtgttg cgcccagtgt cagggctttc
     2641 aactgaatgc atatcaacag cacggtaatt gctgccgggc aggggataag gccaccggtt
     2701 aagccaaata acaaaatttg ccagttgatg acctctctac catcaaagcg tcgtttaatg
     2761 tcattggcat ggtctcgtgc atgggcatcc tgatactcgc catgttcgtg atggtgatga
     2821 tgtccttggt cgtggtgatg ttcgtggtct tgatgatgtt cgtgatcgtg atgatggtga
     2881 tgatgctcat aatcatgccc gtgcatattc tccagccagt tgcgttcgcc gcgccaggta
     2941 cgccagaaca tccagaacgc ggtgccaata atgatcactg cggaaatcag ctggagccac
     3001 ggttctgctg attgagcagt aaagcgctta ctgatcacca tcccgccaaa ggcaatcaac
     3061 cagaccaccg cggtatgcga aatggttgct gccagtccga gcatcaccgc ctgtttaatg
     3121 gtgcctttga tggcgatgat aaacgccgcc atcatcgttt ttgagtgccc tggttccagg
     3181 ccatgaagcg caccaagtaa gatggcgctg gggatgaaga accaggcgtt tccttgctga
     3241 agaagagttg taaattcggt catgataata attcttagta ttaattcggc aatctgattc
     3301 tactcccccc cagtacctga tactactccc ccagtagatt aatagtgcta tgatttttca
     3361 tattcttgtt aaccaggtgt tgccatgtct catacaatcc gtgataaaca gaaactgaaa
     3421 gcgcgtgcca gtaagattca gggccaggtc gtggcgctca agaaaatgct cgacgagccg
     3481 cacgaatgcg ctgcagtttt acaacagatt gctgctatcc gtggagcggt aaacggtctg
     3541 atgcgggaag tgattaaagg tcatctgacg gaacacatcg ttcaccaggg ggatgagcta
     3601 aaacgtgaag aagatctgga tgtcgttctg aaggtgctgg attcatatat caaataattt
     3661 attaacgcga ttgtaaaact gccgtttttc ctcgtttaca acgggtgcgc tggacattac
     3721 catcctcctc tgcgatttat catcgcaacc aaacgactcg gggtgccctt ctgcgtgaag
     3781 gctgagaaat acccgtatca cctgatctgg ataatgccag cgtagggaag tcacggacca
     3841 ccaggtcatt gcttcttcac gttatggcag gagcaaacta tgcaagtcga cctgctgagt
     3901 tcagcgcaat ctgcgcacgc gttacacctt tttcaccaac attcccctct tgtgcactgc
     3961 atgaccaatg atgtggtgca aacctttacc gccaatacct tgctggcgct cggtgcatcg
     4021 ccagcgatgg ttatcgaaac cgaagaggcc agtcagtttg cggctatcgc cagtgccttg
     4081 ttgattaacg ttggcacact gacgcagcca cgcgcacagg cgatgcgtgc tgccgttgag
     4141 caagcaaaaa gctctcaaac acgctggacg cttgatccag tagcggttgg tgcgctcgat
     4201 tatcgccgcc atttttgtca tgaactttta tcttttaaac cggcagcgat acgtggtaat
     4261 gcttcggaaa tcatggcatt agctggcatt gctaatggcg gacggggagt ggataccact
     4321 gacgccgcag ctaacgcgat acccgctgca caaacactgg cacgggaaac tggcgcaatc
     4381 gtcgtggtca ctggcgaggt ggattatgtt accgatggac atcgtatcat tggtattcac
     4441 ggtggcgatc cgttaatgac caaagtggta ggaactggct gtgcattatc ggcggttgtc
     4501 gctgcctgct gtgcgttacc aggcgatacg ctggaaaatg tcgcatctgc ctgtcactgg
     4561 atgaaacaag ccggagaacg cgcagtcgcc agaagcgagg ggccaggcag ttttgttcca
     4621 catttccttg atgcgctctg gcaattgacg caggaggtgc aggcatgaaa cgaattaacg
     4681 ctctgacgat tgccggtact gatccgagtg gtggtgcggg gattcaggcc gatcttaaaa
     4741 ccttctctgc acttggcgct tatggttgct cagttattac tgcactggtg gcgcaaaata
     4801 cccgtggcgt acagtcggtg tatcgcattg agcctgattt tgtcgccgcc cagctcgatt
     4861 cggtgttcag cgatgtgcga atcgatacca ctaaaatcgg tatgttggcg gaaaccgata
     4921 ttgttgaagc ggtggcagaa cggttgcaac gttatcagat ccaaaacgtg gtactcgaca
     4981 ccgttatgct ggcaaaaagc ggcgacccgc tgctttcacc ttcggcggtt gctacgctgc
     5041 gcagtcgatt attgccacag gtttcattaa taacgccaaa cttgcccgaa gctgccgcct
     5101 tgctcgacgc gccacacgcg cgcaccgaac aggaaatgct ggaacaaggg cgatcgctgt
     5161 tggcgatggg ctgtggcgca gtgctaatga aaggtggtca tctggatgat gagcaaagcc
     5221 cggactggct gtttacccgc gagggtgaac aacggtttac cgcaccgcgc attatgacca
     5281 aaaacaccca cggcactggt tgtacactct ctgcggcgtt ggctgcacta cgcccgcgcc
     5341 atacaaactg ggctgacacc gtacaggagg caaaaagctg gctttcatcg gcgttagccc
     5401 aggccgacac gctggaagtt ggtcacggta ttggtccggt tcaccacttc cacgcctggt
     5461 ggtgagcacc gtactatagc tgctttggtt attgtcagcc tttggcactg acattcaggg
     5521 atgaaaccaa tgcagctaag aattaccagt cgaaaaaaac tcaccgctct attatgcgct
     5581 cttgggctaa tttccatcgt tgcaatttat ccgcgtcaga cggtgaattt tttctattcg
     5641 acagcagttc agattacaga ctacatccac ttctacggtt atcgcccggt taaatctttc
     5701 gctattcgca ttcctgccag ttacaccatt cacggattag atgtttcgcg ctggcaggag
     5761 cgtatcgact ggcagtgggt ggcaaaaatg cgcgacaacg atatccgctt acagtttgct
     5821 tttattaagg ctacggaagg cgaaaagctg gtggacccct atttttcgcg taactggcaa
     5881 ttaagccgcg aaaatggcct gctgcgcggg gcgtatcatt atttctcccc gtcggtatcc
     5941 gcttcagttc aggcgcgatt atttctgcaa acggtggatt tctcacaagg cgatttccct
     6001 gccgtgctgg acgtagaaga acggggaaaa ttatcggcaa aagaattacg caagcgggta
     6061 agtcagtggc taaaaatggt cgaaaaacgc acgggaaaaa agccgattat ttactcagga
     6121 gccgtttttt atcacaccaa tctggcgggc tatttcaatg aatatccgtg gtgggtggct
     6181 cactattatc aacgtcgtcc ggacaatgac ggcatggcct ggcgcttctg gcagcattcc
     6241 gaccgtggac aggtagatgg catcaatggt ccggtggatt ttaatgtgtt taatggcacg
     6301 gtggatgagt tgcaggcatt cgttgatggg attaaagaaa cgccttaaat aagaagcagt
     6361 gatatactcc caccagactc cctggcagga aaaagacaaa tggaacaagc gcatacccag
     6421 ctgattgccc aactgaacga gcgtatttta gcggcggata acacgccgct ttatattaag
     6481 tttgccgaaa cggtaaaaaa tgccgtgcgc agcggggtgc tggagcatgg caatattttg
     6541 cccggtgagc gtgatttaag tcagttaacc ggcgtttcgc gcattacggt gcgcaaggcg
     6601 atgcaggcgc tggaagaaga gggcgtggtg acgcgttcgc gcggttacgg cacgcagatc
     6661 aacaacatct tcgaatactc gttgaaagaa gcgcgaggtt tttctcagca ggtggtattg
     6721 cgcgggaaaa agcccgatac gctatgggtt aataagcgtg tcgtgaaatg tcccgaagaa
     6781 gtcgcgcagc agctggcggt cgaagcggga agtgatgtct ttttgcttaa gcgtattcgc
     6841 tatgtcgatg aagaagcggt atcgattgag gaatcgtggg ttccggcgca tttaattcat
     6901 gatgttgatg ccatcggaat ttctctttat gactatttcc gcagccagca catttaccca
     6961 caacgtacgc gttcccacgt tagcgcccgg atgccggatg ccgagtttca gtcacatatt
     7021 cagttagata gcaaaatacc ggtgctggtg atcaagcaag tggcgcttga ccaacagcaa
     7081 cggcctattg agtacagcat tagccactgt cgcagcgatc tatacgtttt tgtgtgcgag
     7141 gagtagttcc tcgcgcgttg gcgcacaatc accgccccga tgcccgacaa cccacgacgc
     7201 cactgcattg cccagtaata cggcatccgc cagtggcaga ccagaggcca gcccggcaag
     7261 tacgccacct gcatgactgt cgcccgcccc aatggtgtct acaacttgcg ttggaaatgc
     7321 cggaatgcag ccagaagcgt cgttgctgaa ataccatgcg ccttctttat cgagacgaac
     7381 gatcaacggc gcggcaaatt tctcctgcca ttgcttgcca agtgttgtta tctctgcgga
     7441 taaagcaaaa cgttcggcgg caatctcagc ctcttgacga ttgagtgaca ctaaaggtcg
     7501 acaggccatg atccgcgcca gtaatgcgtc cgggatatcg ccaatacgtg ggccaaaatc
     7561 gataaacggc gtcacgtctt gcagcttttc cagccattcc actaacaatt cgccgcaggg
     7621 cgaggccagt tgataaccgg aaaaatagag caggttgcca ggcgcaacgg ttaatcgtgc
     7681 caaccattgg cgattccatt gattttccac accgctgaat gacataaaag tgcgttcgcc
     7741 atccggctca accagtgcca gacaccagcc gttatcgctt tcggcgttat cgatcaggct
     7801 gattaacccc tctttcgcca tccggttgcg aataatctcc gcccacacgc cctgaccgag
     7861 cggcaaggca ttacccgctt cgatgccgag gcgctttaac gccacggcaa tattcagtgc
     7921 gcagccgcca acgttaacgc tctgctgttt cagttcgata tcgcacccac gccaggggag
     7981 ggcataagcg tcggcgatca cgtcaatgac cgccgcgccg acgaccatca ccggctgacg
     8041 cgtggttaat tcaggcagca gtgtgtgtaa tcgagcgccg ctcataccgc ctcccgttgt
     8101 tgacgatatt tcgccagcgc tgtggcatag cggttgaaat caagctgatt taccgcatcc
     8161 agctccgcct ttaacgcagg atcgacagcg ttaatgccat gcaacgcgcc gcaaatcgcc
     8221 gtcgccatag cacctatggt gtctgtgtcg ccgccaagat tggcgcacag gacggcgcag
     8281 cggtttgggt cggtttgtgc cagttcaact agcgcaatgg cgcacggaac ggactcaata
     8341 gtgctggtgc ctgcgccaac gacctggtaa agctgttcgc tggcggattc ggtgccgtcg
     8401 gcattacgca caattttcag cgcaatctcc agacgtgccg ccagtgaggc gctgaatgtg
     8461 gtggtgcgtt tttgctgtgc atgtcgcgca attgaaggca atgaatcaac aatcgctgac
     8521 cagcgttctc cgtcaatggc acgagaaatc gcccatgcga tgactaccgc gcctgcaacc
     8581 gccagatcgg atttatgggt cgggctggac gccagcgcca catcatcaat aaaggaatca
     8641 acatcacgcg ctggaagcaa acaacctaat ggcgagacgc gcatcgctgc gccgttggtt
     8701 acgccgttgt tttccagttc agcaacgggt ttaccgtcgc gaatggcgtt aagcgcaatc
     8761 ttcgaggtcg gacctagtac gtttttgtta aaggcgtcga aacgcaacgc ccagtcgaga
     8821 atattacgcc caatcagatt cggatcgatc ttgccttcac attccagtaa cgcatccgcc
     8881 agacacaggc catcgaggta tcgtcggtga attcggcgcg gttaaaataa caggccgcgt
     8941 tattctcctt tggtccaggc agaaaacggt caatccagcc aaagtgtgct ttaacgcggc
     9001 tgcgtggcca aagctcggag ggcatcccca tcgcatcccc taacgcctgc ccataaagag
     9061 caccgagaat acgttctgtt ttcatttaac ttccccttgt gtcaacgcaa tatctcgatc
     9121 atcgaccttg atagccgtaa tttcgttgtc ggattcgcgg aaaaaaatca tgaacagcac
     9181 ggcgataatg gcaatcatca ccgcgccgaa agtccacatc ccggaccagt tgaaagtcag
     9241 tccgtttacc ggttcctgat aagcgaacat cttttccatc atcacaccgc caagacgata
     9301 gccgagcaaa ctgccgaagc cttggcagca gagcgtgatc agcccctgcg cagcggtacg
     9361 catatgcacg ggggcttttt tatcgacata gatgtaagcg gtaacgtagt aaaaatcgta
     9421 acttacgccg tgaagcaaaa taccgaggaa cagtaacgcg taggtgaaat attcatccgc
     9481 actaccgtaa ataaagaagc catagcagat cgcagcggtg accagaccaa gcaataatac
     9541 ctttttgata ccaaagcgtt tagtgaaaaa cggcaatgcc agcataaaga agatttcaga
     9601 gaactggccg agcgtcatcc agccggtggc gtttttcatg ccaacttcgg tcagataacc
     9661 gttggcaaag atgtaataga acgccagtgg catcgcaaac aggaatgaac agaagaaaaa
     9721 gacgaggaag tttttatcgc gcagcaggat cagcgcatcc aggccgagca tgactttaat
     9781 gtccattttg ccggtgcttt ttggcggcgt gttgggcagg aaaaacgcaa acacaccgag
     9841 cagagcagaa cttccggcgg taatcagcag cgggatgtta gtcggtgaga tatcggcata
     9901 ccccagcatt tgcggcaaga aaccacatgc cagaccggag gcaatccagc cgatagtgcc
     9961 catcacacga atgcgtggga aatcacgttc cacatccggc acgttggcaa aagcgatgct
    10021 gttagtcagc gcaatggtcg gcatataggt tagcgagtag gccagcagta acggaaagaa
    10081 cccggcaaaa gtggtctgtt gcgcagcgaa atacatcagc acagcaccag cgaacatcaa
    10141 taccgccagc actttctgcg ccgagaaaaa gcggtcagtg atggagccaa ccagaattgg
    10201 cgacaggatc gccgcaatgg ctgtgcaggc atacgaccag ccaatttctc cggcactaaa
    10261 accgctttta cttaaccaca accacaatgg cacaaaccac gcgccccaga taaaccattc
    10321 aacaaacatc atgaacgaca gctttgctgt tgttttcatc ttttaattcc ttcatgacag
    10381 gtaatgtaag gtacgcgatg acaataccac ctaataatgc cttttgagta cctttgcggt
    10441 gaaaacttga ccgcgctaac attttttctg atgaatcgag ccaacagaaa acgctgaaaa
    10501 aacatccaaa agatggaaaa actcgcccgt aacgaccagg attatgattg cccgcaaaag
    10561 gcgggccagg acaatccata gccgatatcc aatcggaatt tacgggagca tagtaatgac
    10621 agatattgcg cagttgcttg gcaaagacgc cgacaacctt ttacagcacc gttgtatgac
    10681 tattccttct gaccagcttt atctccccgg acatgactac gtagaccgcg tgatgattga
    10741 caataatcgc ccgccagcgg tgttacgtaa tatgcagacg ttgtataaca ccgggcgtct
    10801 ggctggcaca ggatatcttt ctattctgcc ggttgaccag ggcgttgagc actctgccgg
    10861 agcttcattt gctgctaacc cgctctactt tgacccgaaa aacattgttg aactggcgat
    10921 cgaagcgggc tgtaactgtg tggcatcaac ttacggcgtg ttggtgtcgg tatcgcggcg
    10981 ctatgcgcat cgcattccat ttctcgtcaa acttaatcac aacgagacgc taagttaccc
    11041 gaatacctac gatcaaacgc tgtatgccag cgtggagcag gccttcaaca tgggcgcggt
    11101 ggcggttggt gcgactatct attttggctc ggaagagtca cgtcgccaga ttgaagaaat
    11161 ttctgcggcc tttgaacgcg cgcacgagct gggcatggtg acagtgctgt gggcctattt
    11221 gcgaaactcc gcctttaaga aagatggcgt tgattaccat gtttccgccg acctgactgg
    11281 tcaggcgaac catctggcgg caaccatagg tgcagatatc gtcaaacaaa aaatggcgga
    11341 aaataacggc ggctataaag cgattaatta cggttatacc gacgatcgcg tgtacagcaa
    11401 gttaaccagc gaaaacccga ttgatctggt gcgttatcag ttagctaact gctatatggg
    11461 ccgggctggg ttgataaact ccggcggtgc tgcaggcggt gaaactgacc tcagcgatgc
    11521 agtgcgtact gcg
//
LOCUS       DBKEKI010000124        11355 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.124, whole genome shotgun sequence.
ACCESSION   DBKEKI010000124 DBKEKI010000000
VERSION     DBKEKI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.124"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            597..3020
                     /gene="pgaA"
                     /locus_tag="ACUQPX_003291"
                     /pseudo
     CDS             597..3020
                     /gene="pgaA"
                     /locus_tag="ACUQPX_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            3029..5047
                     /gene="pgaB"
                     /locus_tag="ACUQPX_003292"
                     /pseudo
     CDS             3029..5047
                     /gene="pgaB"
                     /locus_tag="ACUQPX_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
     gene            5040..6365
                     /gene="pgaC"
                     /locus_tag="ACUQPX_003293"
     CDS             5040..6365
                     /gene="pgaC"
                     /locus_tag="ACUQPX_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HHV9057892.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQKQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLAGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6367..6780
                     /gene="pgaD"
                     /locus_tag="ACUQPX_003294"
     CDS             6367..6780
                     /gene="pgaD"
                     /locus_tag="ACUQPX_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HHV9057893.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6830..7618)
                     /gene="phoH"
                     /locus_tag="ACUQPX_003295"
     CDS             complement(6830..7618)
                     /gene="phoH"
                     /locus_tag="ACUQPX_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HHV9057894.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8226..9491)
                     /gene="efeB"
                     /locus_tag="ACUQPX_003296"
     CDS             complement(8226..9491)
                     /gene="efeB"
                     /locus_tag="ACUQPX_003296"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HHV9057895.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGSYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(9497..10624)
                     /gene="efeO"
                     /locus_tag="ACUQPX_003297"
     CDS             complement(9497..10624)
                     /gene="efeO"
                     /locus_tag="ACUQPX_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HHV9057896.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10682..>11355)
                     /gene="efeU"
                     /locus_tag="ACUQPX_003298"
     CDS             complement(10682..>11355)
                     /gene="efeU"
                     /locus_tag="ACUQPX_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HHV9057897.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         2969 a         2777 c         2659 g         2950 t
ORIGIN      
        1 ctttttccat aaagctacac caatctgtag gttgtagatc cctttctatt attaatagat
       61 ataagaactg tactgttgtc aatatgaaat tccgcccaca aacaataccg atgatgatag
      121 ttgctgaatg taattattat tgcctaagtg ctttatacat tgtttgcaac aagccaggct
      181 tgaaaatact tataaatggt tatagaaaat ccttataaaa cattaattca tagatacatt
      241 aggaataaca attaaatccg tgagtgccgt agcgcagcct ttcatcagga ctttcgaaaa
      301 atccgaaatc atgcatcgga atttactgat ttaattattt taatcctaat ttattttgaa
      361 aaaggcattg ggatttatgc cgtattcctg aagatcctca tcattggaat ggattttcgg
      421 gcgagaaaag gattttatat ggacactctg ctcatcattt cttcttctca tcatcaacaa
      481 ttcacgtctc tcttccgcgt ttaataacgg attatgaggt gcaaaaatat ctttcttttc
      541 agttacctgt aattagatac agagagagat tttggcaata catggagtaa tacaggatgt
      601 attcaagtag cagaaaaagg tgcccgaaaa ccaaatgggc tttgaaactt cttactgccg
      661 catttttagc agcgagtccc gcggcgaaga gtgctgttaa taacgcctat gatgcattga
      721 ttattgaagc tcgcaagggt aatactcagc cagctttgtc atggtttgca ctaaaatcag
      781 cactcagcaa taaccaaatt gctgactggt tacagattgc cttatgggcc gggcaagata
      841 aacaggttat taccgtttac aaccgctacc gtcctcagca attaccagcg cgtggttatg
      901 cagctgtcgc cgtcgcttat cgtaacctgc aacaatggca aaactcgctt acactgtggc
      961 aaaaggcgct ctctctggag ccgcaaaata aggattatca acggggacaa attttaaccc
     1021 tggcagatgc gggtcactat gatactgcgc tggttaaact taagcagctt aactctggag
     1081 caccggacaa agccaattta ctcgcagaag cctatatcta taaactggcg gggcgtcatc
     1141 aggatgaatt acgggcgatg acagagtcat tacctgaaaa tgcatctacg caacaatatc
     1201 ccacagaata cgtgcaggca ttacgtaata atcaacttgc tgccgcgatt gacgatgcca
     1261 atttaacgcc agatattcgc gctgatattc atgccgaact ggtcagactg tcgtttatgc
     1321 ctacgcgcag tgaaagtgaa cgttatgcca ttgccgatcg cgccctcgcc caatacgctg
     1381 cattagaaat tctgtggcac gataacccag accgcactgc ccagtaccag cgtattcagg
     1441 ttgatcatct tggcgcgtta ttaactcgcg atcgttataa agacgttatt tctcactatc
     1501 agcgattaaa aaagacgggg caaattattc cgccctaggg gcaatattgg gttgcatcgg
     1561 cttatctcaa agatcatcag ccgaaaaaag cacagtcaat aatgaccgag ctcttttatc
     1621 acaaggagac cattgccccg gatttatccg atgaagaact tgcggatctc ttttacagcc
     1681 acctggagag tgaaaattat ccgggcgcgc taactgtcac ccaacatacc attaatactt
     1741 cgccgccttt ccttcggtta atgggcacgc ctacgagcat cccgaatgat acctggttac
     1801 aggggcattc gtttctctca accgtagcaa aatatagtaa tgatcttcct caggctgaaa
     1861 tgacagccag agagcttgct tataacgcac caggaaatca gggactgcgc attgattacg
     1921 cgagtgtgtt acaagcccgc ggttggcctc gtgcagcaga aaatgaatta aaaaaagcag
     1981 aagtgatcga gccacgtaat attaatctgg aggttgaaca agcctagaca gcattaacgt
     2041 tacaagaatg gcagcaggca gctgtcttaa cgcacgatgt tgtcgaacgt gaaccgcaag
     2101 atcccggcgt tgtacgttta aaacgtgcgg ttgatgtaca taatcttgca gagcttcgta
     2161 tagctggttc aacaggaatt gatgccgaag gcccggacag tggtaaacat gatgtcgact
     2221 taaccaccat cgtttattca ccaccgctga aggataactg gcgcggtttt gctggattcg
     2281 gttatgccga tggacaattt agcgaaggaa aagggattgt tcgcgactgg ctggcgggtg
     2341 ttgagtggcg gtcacgtaat atctggctcg aggcggagta cgctgaacgc tttttcaatc
     2401 atgagcataa acccggcgcg cgcctgtctg gctggtatga ttttaatgat aactggcgta
     2461 ttggttcgca actggaacgc ctctctcacc gcgttccatt acgggcaatg aaaaatggtg
     2521 ttacaggcaa cagtgctcag gcttatgttc gctggtatca aaatgagcgg cgtaagtacg
     2581 gtgtctcctg ggctttcact gatttttccg acagtaacca gcgtcatgaa gtctcaattg
     2641 agggtcagga acgcatctgg tcttcaccat atttgattgt cgatttccta cccagtctgt
     2701 attacgaaca aaatacagaa cacgataccc catactacaa ccctataaaa acgttcgata
     2761 ttgttccggc atttgaggca agtcatttgt tatggcgaag ctatgaaaat agctgggagc
     2821 aaatcttcag cgcaggtgtt ggtgcctcct ggcaaaaaca ttatggcacg gatgtcgtca
     2881 cccaactcgg ctacgggcaa cgcattagct ggaatgacgt gattgatgct ggcgcaacgc
     2941 tacgctggga aaaacgacct tatgacggtg acagagaaca caacttatac gttgaattcg
     3001 atatgacatt cagattttaa ggataaatat gttacgtaat ggaaataaat atctcctgat
     3061 gctggtgagt ataattatgc tcaccgcgtg catcagccag tcaagaacat catttatacc
     3121 gccacaggat cgcgaatctt tactcgccga gcaaccgtgg ccgcataatg gttttgtggc
     3181 gatttcatgg cataacgttg aagacgaagc tgccgaccag cgttttatgt cagtgcggac
     3241 atcagcactg cgtgaacaat ttgcctggct gcgcgagaac ggttatcaac cggtcagtat
     3301 tgctcaaatt cgtgaagcac atcgaggagg aaaaccgcta ccggaaaaag cagtagtgct
     3361 gacttttgat gacggctacc agagttttta tacccgcgtc ttcccaattc ttcaggcctt
     3421 ccagtggcct gctgtatggg cccccgtcgg cagttgggtc gatacgccag tggatgaaca
     3481 agtaaagttt ggcgatgaga tggtcgatcg agaatatttt gccacgtggc aacaagtgcg
     3541 agaagttgcg cgttcccggc tcgttgaggt cgcttctcat acatggaatt ctcactacgg
     3601 tattcaggct aattccaccg gcagcttaat gcctgtatat gtaaatcgtg catattttac
     3661 tgaccacgca cggtatgaaa ccgcagcaga ataccgggaa agaattcgtc tggatgctgt
     3721 aaaaatgacg gaatacctgc gtacaaaggc tgaggtaaat ccacacgttt ttgtttggcc
     3781 ttatggcgaa gcgaatggca tagcgataga ggaattaaaa aaactcggtt atgacatgtt
     3841 tttcaccctt gaatcaggtt tggcaaatgc gtcacaattg gattccattc cgcgagtatt
     3901 aatcgccaat aatccctcat taaaagagtt tgcccagcaa attattaccg tacaggaaaa
     3961 atcaccacaa cggataatgc atatcgatct tgattacgtt tatgacgaaa accgccagca
     4021 aatggatcgc aatattgatg tgctaattca gcgggtgaaa gatatgcaaa tatcaaccgt
     4081 gtatttgcag gcatttgctg atcccgatgg tgatgggctg gtcaaagagg tctggtttcc
     4141 aaatcgtttg ctaccaatga aagcagatat ttttagtcgg gttgcctggc aattacgtac
     4201 ccgctcaggt gtaaacatct atgcgtggat gccggtatta agctgggatt tagatcccac
     4261 attaacgcga gtaaaatact taccaacagg ggagaaaaaa gcacaaattc atcctgaaca
     4321 atatcaccgt ctctctcctt tcgatgacag agtcagagca caagttggca tgttatatga
     4381 agatcttgcc ggacatgctg cttttgatgg catattgttc cacgatgatg ctttgctttc
     4441 agattatgaa gatgccagtg caccggctat cacggcttat cagcaagcag gctttagcgg
     4501 gagtctgagc gaaattcgat aaaacccgga gcaatttaaa cagtgggccc gctttaaaag
     4561 tcgtgcgtta actgacttca ctttagaact tagtgcgcgc gtaaaagcca ttcgcggtcc
     4621 acatattaaa actgcacgaa atatttttgc acttccggta atacaacctg aaagtgaagc
     4681 ctggtttgca cagaattatg ctgatttcct aaaaagctat gactggaccg ctattatggc
     4741 tatgccttat atggaaggtg tcgcagaaaa atcggctgac caatggttaa tacaattgac
     4801 caatcaaatt aaaaacatcc ctcaggctaa agacaaatct attttagaat tacaggcaca
     4861 aaactggcag aaaaatggtc agcatcaggc tatttcttcg caacaactcg ctcactggat
     4921 gagcctatta caactgaatg gagtgaaaaa ctatggttat tatcccgaca attttctgca
     4981 taaccaacct gaaatagacc ttattcgtcc tgagttttca acagcctggt atccgaaaaa
     5041 tgattaatcg catcgtatcg ttttttatat tatgtctggt gttatgcata cccctatgcg
     5101 tagcgtactt tcactctggt gaactgatga tgaggttcgt tttcttctgg ccgtttttta
     5161 tgtccattat gtggattgtt ggcggcgtct atttctgggt ctatcgtgaa cgccactggc
     5221 cgtggggaga aaacgcacca gctccccagt tgaaagataa tccgtctatc tccattatca
     5281 ttccctgttt taatgaggag aaaaacgttg aggaaaccat acacgccgct ttagcacagc
     5341 gttatgagaa cattgaagtt attgccgtaa atgacggttc aacagataaa acccgtgcca
     5401 tcctggatcg catggctgca caaattcccc atttgcgggt cattcatctg gcgcaaaaac
     5461 aggggaaagc cattgcgctt aaaaccggag ctgccgcggc gaaaagtgaa tatctggtgt
     5521 gcattgatgg cgatgcgtta ttagaccgcg atgcggcggc atatattgtg gaaccgatgt
     5581 tgtacaaccc gcgtgtgggt gccgtaaccg gtaatcctcg tattcgaaca cgttctaccc
     5641 tagtgggtaa aattcaggtt ggcgagtatt cctcaattat tggtttgatc aagcgaaccc
     5701 agcgtatcta tggaaacgta tttaccgttt ccggtgttat tgccgcattt cgtcgcagcg
     5761 ccctggcaga agtgggttac tggagtgacg atatgatcac cgaagatatt gatattagct
     5821 ggaagctgca gttgaatcag tggacgattt tttacgagcc acgggcgctg tgctggatat
     5881 taatgcctga aacgttaaaa gggctgtgga aacagcgcct gcgctgggct cagggcggtg
     5941 cagaagtatt cctcaaaaat atgacaaggt tgtggcgcaa agaaaacttt cgaatgtggc
     6001 cgctgttttt tgaatactgc ctgacgacaa tatgggcctt cacctgcctg gccggtttca
     6061 ttatttacgc agtccaactt gccggtgtac cgttaaatat tgaattgaca catatcgctg
     6121 cgacacatac tgccggaata ttattgtgta cgttatgttt actgcaattt attgtcagcc
     6181 tgatgatcga gaatcgctat gagcataatc tgacttcatc gcttttctgg attatttggt
     6241 tcccggttat tttctggatg ctgagcctgg caacgacatt ggtatcattt acacgagtca
     6301 tgttgatgcc taaaaagcaa cgcgcccgtt gggtaagtcc tgatcgcggg attctgagag
     6361 gttaatatga acaatttaat tattacgacc cgacaatcac cagtacgttt actggttgat
     6421 tatgttgcca caaccatctt gtggacatta tttgctttgt tcatattctt attcgccatg
     6481 gatctgctga cgggttatta ctggcaaagc gaggccagaa gccgacttca gttctatttt
     6541 ttgctggcag tggcgaatgc cgtcgtgtta attgtctggg cgctgtacaa taagctgcgt
     6601 tttcaaaaac agcagcatca tgcagcctac caatatacgc cgcaagaata tgcagagagc
     6661 ttagcaatac ctgatgagct ctatcagcaa ctacaaaaaa gccacaggat gagcgtacac
     6721 ttcaccagcc aggggcaaat aaaaatggtt gtttcagaaa aagcgctagt ccgggcataa
     6781 acacccaaaa caaagcccgg ttcgcccggg ctctgcaccg ataatacact taactgtatg
     6841 catgcagcgt acgttggcaa agtgccgaac gtacgcaatc ctcttttccg aaccggacga
     6901 tcccaaccat ttcatcttct tcgaaacgtt ccagcgcgtc acttaatccg gagcacacgc
     6961 cgcgaggcaa atcgcattgc gtgatatcac cgttgacgat aaccgtcacg ttctccccga
     7021 ggcgggttaa aaacattttc atttgcgcgg cagtcacatt ctgcgcctcg tcaagaatga
     7081 cgactgcatt ttcaaaggta cgtccacgca tataggcgaa cggcgcggtt tccaccttcc
     7141 ctatttccgg tcgcaggcag tactgcataa aggaagcccc taagcgccgg accagcacgt
     7201 cgtagaccgg gcgaaaatag ggagcaaact tttctgcgat atcaccaggt aagaagccaa
     7261 gatcttcatc ggcttgcaga actggacggg tgacgataat cctgtcgaca tccttatgta
     7321 tcagggcctc tgccgctttt gctgcgctga tccaggtttt tccgcacccg gcttcgcccg
     7381 tggcgaatat cagctgctta ctctcaatag cctgcagata gtgcaattgc gcttcatttc
     7441 gcgcaaggat gggcgaggta tcgcgactgt cgcgggccat accaatggct tctacgccgc
     7501 tcatctgcac aagcgaggtg accgattctt cttcacgctg cttatggctg cgcgaatccc
     7561 gtctcagcac acgttttgcc tcgcgacgag ctttgatcac tgctttttgt cttcccatgg
     7621 agagcacctt gagttgtttg tattcatcac acgcgccgtt ggcagcgcga ttatgcacac
     7681 gaacatcaga gggttggctt ccttgtaagc catagtttgc ttttggataa aatgccgaaa
     7741 aacggctacg cacaccgttt gcggcgtcgg taacacgagt aaagaaagga agtgagaaaa
     7801 atacagagtg acgaagttgc taccggagga gaaatctccg cgagtggtgg aacgttgctt
     7861 aacaaaacat tgtccagaac aggacgttac catccgcgat ctccatagtg actgactatc
     7921 actgccggga acttccgctg ctacttaata agtacaacag aactcgcatt cattgcaaca
     7981 atatattcac ttatatttaa cgataaatac ccatttcggt gacattaatt tctactggaa
     8041 agatgacaga gtgatgacag tgatggaaaa agctgtgtgc tttccgcagg attcgcaggg
     8101 ctggtaaaat gtgcctcgtg tttgccggat tcggcgcgaa cgccttaccc gacctacaag
     8161 tgattaataa tttgtgcaca aaactcatgc ccgataaacc tgcgcatcgg gcatatttgc
     8221 gtcgttcaaa cctgcaatag cgcccttccc agataatcgt tcgcgtcctt cactcccggc
     8281 agcgaaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg
     8341 agcctttttt gtactgtcag gaagcctttt tccagatcgt gttggtagca gacaaacagc
     8401 aaccccatat ccagttgtcc ggagttggtg acccccaacg aataactgta gccgcgacgc
     8461 agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc
     8521 agcgcgatca ccttcccttc cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc
     8581 attcccagcg gcgcaccggt ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc
     8641 ggcgttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca
     8701 ccgattgtcc acgcaggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac
     8761 ttatcattct ggctatcggg attggcagtg ccgtctttga aacccagcaa attaatcggc
     8821 gtctctttgc ctttactacg cgccgcgtga tcggaaataa acccttcccg cttccagcgc
     8881 acactgagca aatccggcgt gtgtttgatg atatcgcgca gcgcatggat caccgtgtcc
     8941 tgggtgttgg cgcaaatctg tagcaacaca tcaccatgac ataacgccgc atccagcgag
     9001 tcgttgggga aacgcgtcat cttctgcagc ttttttggca tctgtggcgc aaggccaaag
     9061 cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg
     9121 tagctgccaa gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt ttctggtgct
     9181 gctccgccct gactcagaaa agcaaaacgc tgagtcaaca agcgaaacaa ccgctcaaga
     9241 tcggctttat cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc
     9301 gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg tgcatccggt
     9361 gaaagcgtgc ccggtgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc
     9421 agcgcaccaa tccctttcag taaacgtcgg cgtgacggtt cattcacgcc gttttcatct
     9481 ttatactgca taacgcttaa tccagtccca gcacaccgcg aagttgcgcc agatcttccg
     9541 ccagcgcagt aatcggccct ttcagcgcat tccggtcggc atcggtcaat ttgtcgtagg
     9601 tttcaaaacc gtctttagta cggtatttcg ccagaatggt atcgaccttt ttaaagttgg
     9661 catcgacttt tgccaacagt tccgggttgg ctttttgcag ttgtggacgc agcagatcga
     9721 caattttctg cgagccttca acgttagcct ggaaatccca cagatcggtg tggctgtagc
     9781 gatcttcttc accgctgatt ttgctggctg ccacttcctc aatcagtccg gctgcgccgc
     9841 cgaccacttt tgaaggtggg aaagccagtt cactgatgcg tttttgcaaa tcgaccacat
     9901 cggtataaag ctgctcagcg tactgatcca tccctttggt ggtgttgtcg ccaaacaatg
     9961 ctttttccag acggtggaaa ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat
    10021 cttcacgggc gtcaatgctg ccatccagat cggagaacag ttcagcaatc ggttcaatac
    10081 gctcatagtg ctggcgcgtc ggcgcataca gtgctttcgc tttttcgata tcgcctgctt
    10141 taatcgcgtc ggtaaaggct ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat
    10201 acgctttata tgcagtaatt gcaccaccaa gacttaacag cgcatcactt tgcgccgcat
    10261 ccgccgttgc ctcacctttg acgatcaact tccctttcgg gttagtcagc agaccgcagg
    10321 tcatatcgta ttcgccaggc tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga
    10381 tattttcccg ctcttccacc accatcacgc ctttgaggat ctcccactcc agcgccttct
    10441 ggctatggtt ctgaataatg aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt
    10501 cgcactgctt atccgtcacg gtcactttga cctgcggcac atcagcggcg ttagccataa
    10561 aagcagaaga aaacagcgca gccacgctca actgcaatgc gttacggcgg aagttaatgg
    10621 tcatgacgag actatccctt taaagtatgt tgtaactaag caagagtttg cgtcgtattt
    10681 gttacgcgga gcgagacgct gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg
    10741 ccgggatgag ataaataaac cagacggcga cttcgctgac gctcggtgct tcctgatagc
    10801 caaaaatccc ttccatcagc gtgccaaaca gcgagtgagt tgagaggacc gcgctcatat
    10861 cgaaggcgat ttcctgaaag tggttccaca atccggcttc atgaaatgcg cgaatggcac
    10921 cagccgccag ccctgcggcg acgaagagaa taaacaggct ggtccattta aaaaatgcac
    10981 caagattgag gcgaataccg ccccagtaga gcaggaagcc gagcaccacg gcagtagcaa
    11041 gaccgagcat tgcacccagc ggcggccaga tcccgacatc ttgttgaaat gccgccagca
    11101 ggaaaaagac cgactccagc ccttcccttg caacggcaaa aaagaccatc atcaccagcg
    11161 cccagccatg atgatttcca cgctgcaatg cgctatcgac tgcctgttcc agttgcactt
    11221 tgacgttgcg cgacactttg cgcatccaga aaaccatcca ggtaaggatc accacggcga
    11281 tcaccgccac gataccttca aacagttcct gctctttttg cggaaattcg ccggtggttt
    11341 cgttaatgaa gatcc
//
LOCUS       DBKEKI010000125        11280 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.126, whole genome shotgun sequence.
ACCESSION   DBKEKI010000125 DBKEKI010000000
VERSION     DBKEKI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.126"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            51..5291
                     /gene="iatD"
                     /locus_tag="ACUQPX_003299"
     CDS             51..5291
                     /gene="iatD"
                     /locus_tag="ACUQPX_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
                     /protein_id="HHV9057898.1"
                     /translation="MASTNAHQIKNNDQNSLCGLGDKIRRLTAGVCLFTQIFFPVMAT
                     AQNVVHAKPQTTVSSAPPLLENNTVPYTLGALESAQSVADRFGISLEELRRLNQFRTF
                     ARGFDNVRQGEELDVPATTSQKSHEQQNAVPPANGENTLENQIASTSQRVGTLLSQDM
                     NSEQASGMTRGWASSEASGAMTDWLNNFGTARISLGVDEDFSLKNSQFDFLHPWYDTP
                     DYLLFSQHTLHRTDDRTQINTSLGWRHFTSSWMSGINLFFDHDLSRYHSRAGLGAEYW
                     RDYLKLSSNAYIGLTGWRSAPELDYDYEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFDKNDRQSNPHAITAGLNYTPFPLLTLSAEQRQGKQGENDTRFAVDLTWQP
                     SSSMQKQLNPDEVAGRRSLAGSRYDLIDRNNNIVLEYRKKELIRLSLLDPVKGKSGEI
                     KPLVSSLQTKYALKGYNIEAAALKAAGGKVSTSGKDITVTLPGYRFTNTPETDNTWSI
                     DVTAEDVKGNLSRHEQSMVVIQAPTLSQKDSLLSVNPLTVAADKKSTTTLTVTAHDSD
                     GTPVPGLALQTRSEGVQDITLSDWTDNGDGSYIQMLTAGTTSGSVTLTPQINGESAVK
                     ESIVVNIVPVVSSRDHSSITIDNVSYYAGDDIKVRVELKDDSNQPVAYQKEELVKAVT
                     VENSKPGATIVWHEEQPGVYAANYPAHKQGTALRAQLSLHNWNAPLQSHIYNIEANQN
                     KARVATLSATNNDVYADKKTFNTLTINVTDESDNPLTNHQVTFKNEKGSAEFVEPPQQ
                     NTDGYGVATINMVSQVAEENTISATLPNGFSQRIIAKFVSDSSTPKFKQLVADPDTII
                     AGNSQGSTLTAIVTDFHNNPLKDMKVNFVAPGGSQLDNTTATTDQSGIVRVHLTSSKA
                     GSYSVDASLEVDKNIHQSVTITVVPNREQSVMTLNAGSGSAIANNTNIVTLTASVKDV
                     YGHPLPDEDVKFTLPASMTGNLTLSSETARTDANGDAVVTLRGTKAGEFTVTATLTRN
                     NTVAYQQVTFIGDTNSAQLQPLTASLNSIVAGNSTGSTLTATILDAYQNPLKDQLVTF
                     QSNDVTLSETEVTTNTLGQATVTMTSNIAGQHNVVVSRKAQASDNKTFSLSVLPDESS
                     AKVISITGAEKTITVGKNITLRILVQDAFNNVIAGQRVRLSAQPTTNITIGDTAYTDN
                     NGYAYVNLLSTQPGVYQVTATLDNNSSSKVDVNVANGKLELTSSKPETTVHNSEGITL
                     TATARNARGELMPGQIITFSVTPEGATLSNTGEVLTDQSGQAKVTLTSDKVNVYTVTA
                     IMGKDVPVQSQVTVAVKADAKTAHVVSVVASPDTITADGIDSSTITSRVEDDYGFPVE
                     GVDVSHGLDTKGSPVVNIPTTRTDQSGQVTATITNTLAETLTVNVQVPGTANQSATIT
                     LVAGTADESKSILKSDVDTLKADYQQSAKLTLTLQDKYGNPIVTSDHLEFVQSGPFVN
                     FLKLSDIDYSQRNYGEYTVTVTGGKEGTATLIPMLNGVHQANLSVSLNLIHSIKEMSG
                     HVTANNHTFSTAKFPSEGFAGAYYTLNNDNFEAGKTVDDYMFSSSQSWVSVDASGKVS
                     FANIGDQTSVTISAVPRQGGTTYQTLIKLKGWWVNNGNHTNIWLAANALCHAKNDGYN
                     LPGITHLTSGENKRTQGSLYGEWGNVGAFSSNSQFTPGAYWTSESDDYSRHYYVQMLT
                     GMTGSDADSSPQLTACRKSL"
     gene            complement(5415..5999)
                     /gene="kptA"
                     /locus_tag="ACUQPX_003300"
     CDS             complement(5415..5999)
                     /gene="kptA"
                     /locus_tag="ACUQPX_003300"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HHV9057899.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            6248..6931
                     /locus_tag="ACUQPX_003301"
     CDS             6248..6931
                     /locus_tag="ACUQPX_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HHV9057900.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            6928..7389
                     /locus_tag="ACUQPX_003302"
     CDS             6928..7389
                     /locus_tag="ACUQPX_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HHV9057901.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTA
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            7402..8574
                     /gene="iadA"
                     /locus_tag="ACUQPX_003303"
     CDS             7402..8574
                     /gene="iadA"
                     /locus_tag="ACUQPX_003303"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HHV9057902.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEADVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            8639..9550
                     /gene="hypT"
                     /locus_tag="ACUQPX_003304"
                     /gene_synonym="qseD"
     CDS             8639..9550
                     /gene="hypT"
                     /locus_tag="ACUQPX_003304"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HHV9057903.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(9543..9935)
                     /gene="iraD"
                     /locus_tag="ACUQPX_003305"
     CDS             complement(9543..9935)
                     /gene="iraD"
                     /locus_tag="ACUQPX_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HHV9057904.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVYNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(9932..10015)
                     /gene="idlP"
                     /locus_tag="ACUQPX_003306"
     CDS             complement(9932..10015)
                     /gene="idlP"
                     /locus_tag="ACUQPX_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HHV9057905.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(10131..10274)
                     /gene="ytiD"
                     /locus_tag="ACUQPX_003307"
     CDS             complement(10131..10274)
                     /gene="ytiD"
                     /locus_tag="ACUQPX_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HHV9057906.1"
                     /translation="MADYAEINNFPPKLSSSGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
     gene            complement(10271..10354)
                     /locus_tag="ACUQPX_003308"
     CDS             complement(10271..10354)
                     /locus_tag="ACUQPX_003308"
                     /inference="COORDINATES: protein motif:HMM:NF048182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057907.1"
                     /translation="MPVNGIFDVFDMLSIYIIYKLIVSNNT"
     gene            10608..>11280
                     /locus_tag="ACUQPX_003309"
     CDS             10608..>11280
                     /locus_tag="ACUQPX_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HHV9057908.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIDI"
BASE COUNT         3050 a         2786 c         2692 g         2752 t
ORIGIN      
        1 tccgtgctag aaaaaacaag tttaattctg ttttttcacg gaaggtcact atggcttcga
       61 caaatgcgca tcaaataaaa aataatgatc aaaactcttt atgtggtctg ggtgacaaaa
      121 tacgccgcct gaccgccgga gtttgtttat ttacacaaat ttttttcccc gtcatggcga
      181 cggcacaaaa tgtggtacat gctaaacctc agacgacagt ttcatctgcc ccccctctac
      241 tagaaaataa tacggtgcct tacacgcttg gtgcgctgga atcagcgcaa agcgttgctg
      301 atcgcttcgg tatttcactg gaggagcttc gtcgtcttaa tcagttccgt acttttgctc
      361 gcggctttga taacgtgcgc cagggtgaag aactggatgt tccggcaaca acctcgcaga
      421 aaagccatga gcaacaaaat gccgtaccgc ctgcgaatgg cgaaaacacg ctggagaatc
      481 aaatagccag cacctcgcag cgagttggca ctctgctttc acaagatatg aatagtgagc
      541 aggccagcgg catgacgcgt ggttgggcgt cttcagaagc ctcaggcgcg atgactgatt
      601 ggttaaataa ctttggtact gcgagaatct ctctgggtgt ggatgaagat tttagcctga
      661 aaaattcgca attcgacttc ctgcatccgt ggtatgacac acccgattat ctgctcttca
      721 gccagcatac ccttcaccga acagacgatc gtacccagat caacaccagt ttgggctggc
      781 gtcatttcac ctccagctgg atgtcaggca tcaacctgtt ttttgaccac gacctgagcc
      841 gctatcactc ccgcgcgggg cttggcgcag aatactggcg tgattatctg aagttgagca
      901 gcaacgctta tattggcctg accggctggc gtagcgcacc agaattggat tatgactacg
      961 aagcccgccc ggccaacggc tgggatttac gcgcggaagg ctggttacct gcctggccac
     1021 aactgggggg aaaactggtc tatgaacaat actatggcga tgaagtggcg ctgtttgaca
     1081 agaatgatcg tcaaagtaac ccccatgcta ttaccgcagg cctcaactat acccccttcc
     1141 cgcttctgac tctcagtgcg gaacagcgtc aggggaagca aggtgaaaat gacacacgtt
     1201 ttgccgttga tctgacctgg caacccagca gttcaatgca gaaacagctt aatccggacg
     1261 aagtggccgg acggcgcagt ctggccggta gtcgttatga cctgattgat cgcaacaaca
     1321 acatcgttct ggaataccgc aagaaagagc tgattcgcct gagtctgctg gatccagtga
     1381 aagggaagtc tggagaaata aaaccgctgg tttcctcgct acagaccaaa tatgccctga
     1441 aaggctataa catcgaagcc gctgcgctga aagctgccgg aggtaaagtc agcacgtctg
     1501 gaaaagatat cacggtcacg ctgccaggtt accgcttcac taacacccca gaaaccgata
     1561 atacatggtc gatagacgtt accgccgagg atgtaaaagg taacctgtca cggcatgaac
     1621 aaagcatggt agttattcag gctccgacat taagccagaa agattctctg ttatccgtca
     1681 atccgctaac cgtggctgca gataaaaaat cgacgaccac attgaccgtt actgcgcacg
     1741 attccgacgg aactccggtg ccggggctgg cgctgcaaac ccgcagtgaa ggcgttcagg
     1801 atatcaccct gtctgactgg acagataacg gtgatggtag ttacatacag atgctgaccg
     1861 ccggaacgac atcaggttca gtaacactga cgccgcaaat taacggtgag agtgcggtaa
     1921 aagaatccat cgtcgttaat atcgtccctg ttgtctcatc ccgcgaccat tcatcaataa
     1981 caattgataa cgtatcgtat tatgccggag acgacatcaa ggttagggtg gaactgaaag
     2041 acgacagcaa tcaaccggtt gcatatcaaa aagaggaatt ggtaaaagcc gttactgtcg
     2101 aaaacagcaa acctggcgcc acgattgtct ggcacgaaga gcagccgggg gtttatgccg
     2161 cgaattatcc ggcccataag caagggactg cactaagggc acaacttagc cttcacaact
     2221 ggaatgctcc actgcaatca catatttata acattgaggc aaaccagaat aaggctcgcg
     2281 tcgccacatt atcagcgacg aataatgacg tttacgctga taaaaagaca tttaataccc
     2341 tcactatcaa cgtcactgat gagagtgata atcccctgac aaatcatcag gtcaccttta
     2401 agaatgaaaa aggaagcgcg gaatttgtcg aaccgccgca gcaaaatacg gatggatacg
     2461 gtgttgccac aatcaacatg gtaagccagg ttgcggaaga aaacacgatt agcgccacgc
     2521 tgccaaatgg tttctcacaa cggataattg cgaaattcgt tagcgattcg agtacgccaa
     2581 aattcaaaca actggttgcc gatccagata ccattattgc tggcaacagc cagggcagta
     2641 ctctgaccgc catcgtcaca gactttcata acaacccgtt aaaagatatg aaagtgaatt
     2701 ttgtggcacc tggtggctcg caactggaca acacgaccgc cacaacagac cagtccggta
     2761 ttgtgcgggt gcacctgacc agttcaaaag ctggtagcta ttccgtcgat gcctcgcttg
     2821 aggtggataa aaatattcac cagtcggtca cgatcaccgt ggtcccaaac agggaacaat
     2881 cggtaatgac cttgaatgcc gggtcgggca gtgcgatcgc taacaataca aatatcgtta
     2941 ccctgactgc cagtgtgaaa gatgtttatg gacacccgtt gccggatgag gatgtgaaat
     3001 ttaccttgcc agcctccatg accgggaacc tcacgctaag tagtgaaacc gcccgcaccg
     3061 atgcaaacgg tgatgccgtg gtcacattgc gaggcacaaa agcgggtgag tttacagtta
     3121 cggcgacgct gaccagaaat aataccgttg cttatcagca agtcactttt attggggata
     3181 caaacagtgc gcagctccag ccgctgactg cctcattaaa ttccattgtt gcgggtaaca
     3241 gtacggggag taccctgacg gcaacgatcc tggacgctta ccaaaatccg cttaaagacc
     3301 agttggtcac tttccagagt aacgatgtca ctctaagcga aacagaagtc accaccaata
     3361 cgctgggtca ggcgacggta acaatgacca gcaatattgc cggacaacat aacgtcgtgg
     3421 tgagccggaa agcgcaagct tccgataata aaacgtttag tttatcagtg ctgccggatg
     3481 aaagttcggc gaaggtaata agtataaccg gagccgaaaa aacgataacg gtgggcaaaa
     3541 acatcacgct acggatactc gtccaggacg cgtttaacaa tgtaatcgcg ggtcaacgcg
     3601 tcagattaag tgcgcagcca acaactaaca ttacgatagg cgatacggct tacaccgata
     3661 ataacggtta tgcgtacgtt aaccttctca gcacccaacc tggggtttat caggtgacgg
     3721 caacgctgga caataacagt agtagtaagg ttgacgtgaa tgtggcaaat ggcaaactcg
     3781 agttaacatc atcgaaacca gaaaccacgg tccataatag tgagggtatt acgctgaccg
     3841 caacggcgag aaatgcgcgg ggtgaattga tgccagggca aattatcacc tttagcgtaa
     3901 cgcctgaagg tgcaacgcta agcaatacag gggaagtcct tactgaccag tcaggtcagg
     3961 ccaaagtgac gctgaccagt gacaaagtga atgtctatac cgttacggcc ataatgggca
     4021 aagatgttcc cgttcagagc caggtaacgg ttgcggttaa ggcagatgct aaaacggcac
     4081 atgttgtgag cgtcgtggct tctcctgaca ccatcaccgc cgacggcatc gatagcagca
     4141 ccatcacttc acgagtagaa gatgattacg gattcccggt tgaaggtgtc gatgttagtc
     4201 atggcttaga caccaaaggc agcccggtag ttaatattcc aactacgcgt accgatcagt
     4261 ccgggcaagt cacggcgaca ataaccaata cattggcaga aaccttaaca gtcaatgtgc
     4321 aagttcctgg tacagccaac caatccgcaa ccattacatt ggttgccggc acggccgatg
     4381 aaagtaagtc aattttgaaa tccgatgttg acactctgaa ggctgactac cagcagagcg
     4441 caaaacttac gctaacattg caagacaagt acggtaaccc gatagtgacg tctgatcatc
     4501 tggaatttgt ccagtcaggc cccttcgtga actttctcaa gttgagcgat attgattaca
     4561 gccaaagaaa ttatggcgag tacaccgtga ctgtcactgg cggaaaagag ggaacagcga
     4621 ccctcattcc catgctgaac ggggttcatc aggcaaactt aagcgtatcg ctgaatctca
     4681 tccactcgat aaaagaaatg tccggtcatg tcactgcaaa caaccatacc ttctccacgg
     4741 ctaaattccc gagcgaaggc tttgcagggg cgtattacac actcaacaat gataactttg
     4801 aagcgggtaa aaccgttgat gattatatgt tttcaagttc acagagttgg gtgtctgtcg
     4861 atgcttcagg taaggtttct ttcgcaaata tcggcgatca aacgtcagtc acaataagcg
     4921 ctgttccccg acaaggaggt acaacctacc agaccttaat taagctgaaa ggctggtggg
     4981 tgaataatgg aaatcatacc aatatctggc tagctgccaa tgcgctctgt catgctaaaa
     5041 atgatggata taatcttcct ggcatcacac atttgacgtc tggcgaaaac aaacgcacgc
     5101 agggatcgct gtatggtgaa tgggggaacg ttggagcgtt ttccagtaat tcgcaattta
     5161 caccgggagc ttactggaca agtgaatctg atgattacag tcggcactac tatgtgcaga
     5221 tgctaaccgg tatgaccgga agcgacgctg attccagccc ccaactgacc gcctgccgta
     5281 aatcacttta atttcagcag ttgttattaa aggataccct tttacgaggg tatcccttca
     5341 gactgataac aaacgctaca ttgccggatg tgctgcgctt accaggccta tataacccct
     5401 gcaatatatt gaatttattg tttttgtacc ccagtcggca taaacgacca ctcaagaaac
     5461 tcaacagcaa ccgttgatgt cagccagacc ccgttttccg cttgccagaa agggatgcct
     5521 cgtttcgcca tttcctgtgc tttgacggtt aagacaaccg gcgatccgtg tcttgctccc
     5581 actttgcgtg ccgttgcttc atcagcagag agatgcacgt aatgacgatc ccctgcaatc
     5641 agcccctgtt tttttatctc gtcaaggaaa cggcttgccg taccgtggta gagaaactgt
     5701 ggcggtgttt tttcggcaaa ggaaatagcg acctgcgaag tcgaatgccc ctgaaccgcg
     5761 cggatgcacc taccatcact ggaataacta aaacgctttt tatcgctggt tgcgaccacg
     5821 gtatccagca atgcacgggt taatcgcttg cccgcttttt gggcacaaag tatgagctta
     5881 ttgatatccg cccatccttc acggtccagt acgataccaa tcgcctccgg tttgtgtcgg
     5941 aggacaaaac ttaaaaactt gctcgtatcc gccagctctt tttcgttgta ctttgccatc
     6001 gcgttttcgt ccctggattt tctgttttta tttactaatg tcattgtcac tgggtttatg
     6061 aattttctgc aactcaatcc tgcgcagaac cttttccaca cagcgtaatt tttccctatg
     6121 caaaaaacgc atagccagaa acatacttgc attttcccca tacccacatg gctgataagg
     6181 tttttcagtt ccaggaattt tcctggctca agcaacacac aaacacaaaa aaacaatggg
     6241 gattgttatg acacaacaag gggatgctgt agccggggag ctggcgacag aaaaagtggg
     6301 cattaaaggg tatctggctt tttttctcac cataatcttt ttttccggtg ttttttccgg
     6361 aacagacagc tggtggcgcg tttttgattt ttccgttctg aacggatcgt ttgggcaact
     6421 gcctggtgcg aatggagcca ccacctcatt tcgcggggtg ggtggcgcgg gggctaaaga
     6481 tggctttctc tttgcgctgg agctggcacc gtcagtcatt ctctctctgg ggattatttc
     6541 aattaccgat ggtcttggtg gattacgcgc cgcgcagcag ttaatgacac ctgtgctaaa
     6601 accgttgttg ggcattccag ggatctgctc gctggcactg atcgctaacc tgcaaaacac
     6661 ggatgccgcc gccggtatga ccaaagagct ggcgcaggaa ggtgaaatta ccgaacgcga
     6721 taaagtgatt ttcgccgcat atcaaaccag cggtagcgcc atcatcacca actacttctc
     6781 ttccggcgtc gcggtgtttg cctttctggg cacgtccgta atcgtacctt tagccgtcat
     6841 cctggtgttt aaatttgtcg gagccaacat tttgcgcgtc tggcttaact tcgaagaacg
     6901 tcgcaaccca acgcaaggag cgcaagcatg acaactcagg tccgcaaaaa cgtcatggat
     6961 atgtttatcg atggcgcgcg ccgtggcttt actatcgcca cgaccaacct gctgccaaac
     7021 gtggtgatgg cgtttgtcat cattcaggcg ctgaaaatta ccggcctgct cgactgggtg
     7081 gggcatatct gtgaacccgt aatggcgctg tggggattac ccggtgaagc ggcaaccgtc
     7141 cttttagccg cattaatgag tatgggcggc gcagtcggtg ttgccgccag tctggcaacc
     7201 gctgacgcat taaccggcca cgatgtcacc gctttactgc ccgcaatgta tttaatgggc
     7261 aacccggtgc aaaatgtcgg tcgctgcctg gggacagccg aagtgaatgc caaatattac
     7321 ccgcatatca ttacggtctg cgtgattaac gcattgctgt cgatctgggt tatgcagctt
     7381 attgtttaac aaggagttac catgattgat tataccgcag ccggttttac cctgctgcag
     7441 ggagcgcatt tgtatgcgcc ggaagatcgg ggaatttgcg atgtcctcgt cgctaacggc
     7501 aaaattatcg ccgttgccag caatatccct tctgacattg taccgaactg cacggttgtc
     7561 gatctcagcg ggcagatcct ctgcccaggt tttattgatc aacacgtcca tttgattggc
     7621 ggtggcggcg aagcaggtcc cacgacgcgc acgccggaag tggcgctaag tcgcctgacg
     7681 gaagcggacg tcacgtcagt ggttggtctg ctgggcaccg actctatctc tcgccacccg
     7741 gaatccctgc tcgccaagac ccgtgcgctc aatgaagaag gcatcagcgc ctggatgctg
     7801 accggcgctt atcatgtccc ttcccgcact attacgggtt ccgtggaaaa agacgtggcg
     7861 attatcgatc gtgtgattgg cgtgaaatgc gccatctctg atcaccgttc tgccgcaccg
     7921 gacgtttatc acctggctaa tatggcggca gaatcccgcg ttggcggttt gctcggcggt
     7981 aaacctggcg tcaccgtgtt ccacatgggc gacagtaaaa aggcgttaaa gcctatttat
     8041 gacctgctgg aaaactgcga tgtgccgatc agcaagctgc tgccgaccca cgttaaccgc
     8101 aacgtaccgt tgtttgagca ggcactggag ttcgcgcgca aaggcggcac catcgatatc
     8161 accagcagca ttgacgaacc ggtcgcccct gccgaaggta ttgcccgcgc cgttcaggcg
     8221 ggtattccgc tggcacgcgt caccctcagc tccgacggca acggtagcca gccgttcttc
     8281 gatgacgaag ggaatttaac ccatatcggt gttgcaggtt ttgaaacgtt gctggaaacc
     8341 gtgcaggtgc tggtcaaaga ctatgatttc agtatcagcg atgccctgcg cccgctcacc
     8401 agtagcgtag ccggtttcct taacatgagc gggaaaggcg aaattctgcc aggcaatgac
     8461 gcagacttac tggtcatgac gccagaattg cgcattgagc aggtatacgc tcgcggcaaa
     8521 ctgatggtca aagacggcaa agcctgcgtg aaaggaacgt ttgaaacggc ttaaacggtt
     8581 gtcagccgcc ctaatggacg tagaatgccc ccaagggcgg ctgacagagt aaaacgtaat
     8641 ggatgactgt ggtgcggttt tgcataatat tgaaaccaaa tggctttatg attttctgac
     8701 tctggaaaaa tgccgcaatt tttcccaggc ggcagtcagt cgcaacgtct cgcaaccggc
     8761 attcagccgc cgcatccgtg cactggaaca ggcgattggc gttgaattgt ttaaccgcca
     8821 agtgacgcca ctgcaactct cagaacaagg gaaaatcttc cattcgcaga tccgccatct
     8881 gttgcaacag ttagaaagca accttgcaga gctgcgtggc ggcagcgatt acgcgcaacg
     8941 taaaatcaag atagccgctg cacactctct ttccctcggt ctgttaccgt ccattatcag
     9001 ccagatgccg ccgctcttta cctgggcaat tgaagccatt gatgtcgatg aagcggtcga
     9061 taaactgcgt gaagggcaaa gtgactgtat tttttccttt cacgacgaag atttgctgga
     9121 agcgccgttt gaccacattc gcttatttga atctcaattg ttccctgtct gcgccagtga
     9181 cgaacacgga gaagcacttt ttaacctcgc gcagccacac tttccgttac tgaattacag
     9241 ccgcaactcc tacatggggc gattgattaa tcgcaccctg acgcgccaca gtgagttaag
     9301 tttcagcacc ttttttgtct cttcgatgag cgagctttta aagcaggttg ccctcgacgg
     9361 ctgtgggatt gcctggctgc cggagtatgc catccaacaa gaaattcgca gcgggcagct
     9421 cgttgtgctg aatcgggacg aacaggtgat cccgattcag gcctacgcat accggatgaa
     9481 tacccgcatg aatccagttg ccgaacgctt ctggcgtgaa ctgcgcgagc tggagattgt
     9541 gcttagctga cattctcaag cgtcgcactg cgccaaccgc cattatccca gaccagatca
     9601 aacgttagcg gttcatcgtc gtggtacagc attatctcaa ggctgatcgc cagagtgtgg
     9661 cagtacgctt ctttcaaatt aacctgcaac gccgcaatcc gtggctcatg aaataacagt
     9721 gcagaacgca gctgacggca ataccagctc tggagatgtt tgtcgctcgg gttatacact
     9781 tgccactcgg gcatgcctga aacaagtaat gacggtaatg ccgaatgagg agaattaaaa
     9841 agagtgagga gatcgctgca taccgattta cgcagcaatg acgttttatt gcctgaaatt
     9901 tcaggtaaaa cagcctgaag tgattggcgc atcattttgc gcactcctga cgtttagcaa
     9961 catatgcggc ttgccctgaa caccgggcac cactgtattg atgctcattt tccatagcca
    10021 ttcccttttg ttcattacat ccatgtcagt ctgagacagg cctgaatcct gtcagcaaag
    10081 tataaaacct tacttgccta tgaatatcta ctgacttttc aggaaaaaag tcatgatttg
    10141 ataaaaatca tcaaactcaa aaaaaaaccg ctagtatatt ctgaatattc cgaatagtta
    10201 cgtagatgaa aatacttgtc accgctgcta cttaatttcg gtgggaaatt atttatttcc
    10261 gcataatcag ctatgtatta tttgaaacaa ttagtttgta aataatataa attgataaca
    10321 tatcaaatac atcaaaaata ccattcacag gcattattaa tatagacctc aatatagcaa
    10381 catcaaattc caattagtaa taagatatgc ttttaaattg catgcaaata attttaaatg
    10441 agcgtttttt gatactttag agtgtttcct accctttaat atcactgtgt agagttgata
    10501 tcactacaac agaaacatgc atgtcatttg tttacttctc tttttactac agaaaaattt
    10561 atctattttt ctaaacatca gttaaatact cataaaaggt aactattatg tctatgccat
    10621 taagcaatgc cgctaataac caattcaata caaataatca cttccttcat catccaaaag
    10681 tcgacagtga gttaacacgt cgatatgaat atgcacgcct ggatacggaa aatatctatc
    10741 ttctgcctct tgctcgtggc aataaccata attacgatgg aaaaagcgtt gtcgaaatta
    10801 gaaaacttaa tattggcaaa cagtcctggc catttaatta tgtgacagat acatgcagag
    10861 aattcaatgg tattacaaca acaggacgta tgctctatcg aaacttgaaa atcacctcag
    10921 ctttagacga aatatatggt ggtatatgta aaaaggcgca tgctgctaca gagttagcag
    10981 aaggtttacg gctaaattta tttatgaaga gccctttcga tcctgttgag gactatacag
    11041 ttcatgaaat aacattaggc cctggttgca atgttcctgg ttatgccgga accacgatag
    11101 gatatatttc aacccttcca gcttctcagg caaaaagatg gaccaatgag cagccaagaa
    11161 ttgatatcta tattgatcag ataataactg tctcaggggt agcaaattct tccggattcg
    11221 cccttgccgc attacttaat gctaatattg agatgggaaa tgacccaatc attgatatag
//
LOCUS       DBKEKI010000126        11161 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.127, whole genome shotgun sequence.
ACCESSION   DBKEKI010000126 DBKEKI010000000
VERSION     DBKEKI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.127"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(364..645)
                     /locus_tag="ACUQPX_003310"
     CDS             complement(364..645)
                     /locus_tag="ACUQPX_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HHV9057909.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(907..2016)
                     /gene="apbC"
                     /locus_tag="ACUQPX_003311"
     CDS             complement(907..2016)
                     /gene="apbC"
                     /locus_tag="ACUQPX_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HHV9057910.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            2148..4181
                     /gene="metG"
                     /locus_tag="ACUQPX_003312"
     CDS             2148..4181
                     /gene="metG"
                     /locus_tag="ACUQPX_003312"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HHV9057911.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERTEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4322..8070
                     /locus_tag="ACUQPX_003313"
                     /pseudo
     CDS             4322..8070
                     /locus_tag="ACUQPX_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            8080..11161
                     /locus_tag="ACUQPX_003314"
                     /pseudo
     CDS             8080..11161
                     /locus_tag="ACUQPX_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532291.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         2967 a         2855 c         2678 g         2661 t
ORIGIN      
        1 tctgcagcaa atactccagc gctcataaaa aaagaagaaa agacagcggc agcaataatt
       61 gaacgtttca taatatgttc cttaataaaa tttatttttg gttgttaaga gtctgtatct
      121 cttggaaaca aatttctcac atcaaaaaag ctgccgcaat gtaaacatat tgttttattg
      181 atttaaaaca aattaaccaa tgcaatataa atacaacaaa tacttaagag tattaaatat
      241 ttatcacatc tattaattaa tgtagattta tgtaaatggt attgttgata tcaataaaaa
      301 agacgaaatc atttatataa atgatttcgt ctttctaaat gcaattaaaa tcaggttgcc
      361 aaattaatcg tagcgattta acacgattcg acgtttgcta tcgctacctg caacaaccac
      421 ttccgtcgta acacctttga cgggcgaaga aacctgatga cgggtgtcaa cattatacgt
      481 taactgacca caatgctgtt gtggttgact attaaccata gccatactgc atgttggggg
      541 actgatcctg ccattaatcg ttaatgtggc agtctctgaa gagactgcgg gagaagccaa
      601 tccataggca agaaaaataa ttcctgacag ccaatactta ttcattgaac gttatccctg
      661 tagtaaaggt tatgcctgga tagaatgagt gcataacaaa ctatagctgt acatccacta
      721 cacagcacga aggatgataa tgaagcattg cctgtacgat cactcgactt tgtagagttt
      781 cggacgaagg tccgcagaat attcgcagta ttaaataagt gttcattaat gcggcaaaaa
      841 caaatacacc gcatcaatat aacatctctg cggtgtatga agaataacca gaatggttaa
      901 taggcgttag accgcgcgga aggaaatctc gcctggaatg acttcaccct gccagtagag
      961 ctgagctgca acgcggtcag ccagctggcg gtagatagcg gtaaattcgc tctccggacg
     1021 gctgataacc gttggcgtgc ctttatcgag atcctcacgt aaggagatgt gtaacggcat
     1081 ctgccccagc agctgtgtat ggtatttttc agccagtttc tctgcaccac cggtaccgaa
     1141 aatcggttca tggtgaccac agttactgca aatatgcaca ctcatgttct cgacaatacc
     1201 gagcaccgga acctcgactt tctcgaacat cacaatgcct tttttcgcat cgatcaacgc
     1261 gatgtcctgc ggggtggtga ccacgactgc accagttact ggaatgtttt gcgccagcgt
     1321 caattgaatg tcaccggtgc ctggcggcat atcaagcacc agatagtcga gatccggcca
     1381 caaggtttcc tgcaacatct gcattaacgc cttgctcgcc attggtccac gccacaccat
     1441 cgcattgtcg tcagtgacca gataaccaat tgaattggtt gccaggccat gagacataat
     1501 cggtgccatg tgggtaccgt ccggcgaagt tggacgctga ttttctgcgc ccagcatggt
     1561 gggaattgat gggccgtaga tatcggcgtc cagaatgccg actttcgccc cttccgccgc
     1621 cagtgccagc gccaggttta ccgccgtgga cgatttaccc acgccgcctt taccggaact
     1681 caccgcgata atgttcttca caccgttaat tcccggctga ttcttcacgc gtttcagagt
     1741 ggctatgtta tgcgacagtt tccagtcgat agctttcgcc ccggtgatgc gcagcagttc
     1801 ggcgctacat tgctctttta actcttcgaa cgcgctatgc cagacaaacg gcataaccag
     1861 ttccacatgc aacgtgtcgt ccatccaggc gacatggtgc aacgctttca gcgtggtgag
     1921 gttatgcttc agggttgggt gctgaaaatt ggccagtgtc ccggcgacca ttgcgcgcaa
     1981 ggcttctggt gatttggcct gggattgttc gttcatcccg actccttttt tgtatagata
     2041 aaccatcagc tgatagttta cctgaagaat atagagaagt acttacttaa cattttccca
     2101 tttggtacta tctaacccct tttcactatt aagaagtaat gcctactatg actcaagtcg
     2161 cgaagaaaat tctggtgacg tgcgcactgc cgtacgctaa cggctcaatc cacctcggcc
     2221 atatgctgga gcacatccag gctgatgtct gggtccgtta ccagcgaatg cgcggccacg
     2281 aggtcaactt catctgcgcc gacgatgccc acggtacacc gatcatgctg aaagctcagc
     2341 agctcggtat caccccggag cagatgattg gcgaaatgag tcaggagcat cagactgatt
     2401 tcgcaggctt taacatcagc tatgacaact atcactcgac gcacagcgaa gagaaccgcc
     2461 agttgtcaga acttatctac tctcgcctga aagaaaacgg ttttattaaa aaccgcacca
     2521 tctctcagct gtacgatccg gaaaaaggca tgttcctgcc ggaccgtttt gtgaaaggca
     2581 cctgcccgaa atgtaaatcc ccggatcaat acggcgataa ctgcgaagtc tgcggcgcga
     2641 cctacagccc gactgaattg atcgagccga aatcggtggt ttctggtgct acaccagtga
     2701 tgcgtgattc tgaacacttc ttctttgatc tgccctcttt cagcgaaatg ctgcaggcat
     2761 ggacccgcag cggtgcgttg caggagcagg tggcaaacaa aatgcaggag tggtttgaat
     2821 ccggcctgca acagtgggat atctcccgcg acgcgccata ctttggtttt gaaattccga
     2881 acgcgccggg caaatatttc tacgtctggc tggacgcacc gattggctac atgggttctt
     2941 tcaagaatct gtgcgacaag cgcggcgaca gcgtaagctt cgatgaatac tggaagaaag
     3001 actccaccgc cgagctgtac cacttcatcg gtaaagatat cgtttacttc cacagcctgt
     3061 tctggcctgc catgctggaa ggcagcaact tccgcaagcc gaccaacctg tttgttcatg
     3121 gctatgtgac ggtgaacggc gcgaagatgt ccaagtctcg cggcaccttt attaaagcca
     3181 gcacctggct gaatcatttt gacgctgaca gcctgcgtta ctactacact gcgaaactct
     3241 cttcgcgcat tgatgatatc gatctcaacc tggaagattt cgttcagcgt gtgaatgccg
     3301 atatcgtgaa caaagtggtt aacctggcct cccgtaatgc gggctttatc aacaagcgtt
     3361 ttgacggtgt gctggcaagc gagctggctg accctgagct gtacaaaacc tttaccgatg
     3421 ccgctgaagt gattggtgaa gcgtgggaaa gccgtgaatt tggtaaagca gtgcgcgaaa
     3481 tcatggcact ggctgacctg gctaaccgct atgtcgacga gcaggctccg tgggtagtcg
     3541 caaaacagga aggtcgcgat gctgacctgc aggcgatttg ctcaatgggc atcaacctgt
     3601 tccgcgtgct gatgacttac ctgaagccgg tactgccgaa actgaccgag cgtacagaag
     3661 ccttcctcaa tacggaactg acctgggatg gtatccagca accgctgctg ggccacaaag
     3721 tgaatccgtt caaggcgctg tataaccgca tcgatatgaa acaggttgaa gctctggtgg
     3781 aagcctctaa agaagaagtg aaagccacta ccgcgccagt tactggccca ctggcagatg
     3841 atccgattca ggaaaccatc acctttgacg acttcgccaa agttgacctg cgcgtggcgc
     3901 tgatcgaaaa cgcagagttt gttgaaggtt ctgacaaact gctgcgcctg acgctggatc
     3961 tcggcggtga aaaacgcaat gtcttctccg gtattcgttc tgcttacccg gatccgcagg
     4021 cactgattgg tcgtcacacc attatggtgg ctaacctggc accacgtaaa atgcgcttcg
     4081 gtatctctga aggcatggtg atggctgccg gtcctggcgg gaaagatatt ttcctgctaa
     4141 gcccggatgc cggtgctaaa ccgggtcatc aggtgaaata atcccccttc aaggcgctgc
     4201 atcgacagcg ccttttcttt ataaattcct aaagttgttt tcttgcgatt ttgtctctct
     4261 ctaacccgta taaatactgg tagcatctgc attcaactgg ataaaattac agggatgcag
     4321 aatgagacac tttatctatc aggacgaaaa atcacataaa ttctggacgg tggagcaaca
     4381 gggaaacgag ttgcatatca gttggggaaa agttggcacc aaagggcaaa gtcagataaa
     4441 aagtttttca gatgctgcgg cggcggaaaa agcggaactg aagctgattg cggagaaggt
     4501 gaagaagggg tatgtggagc aagcgaaaga taattctttg caaccttccc aaacggtaac
     4561 ggactctctc aaggtagcgg acttatccac cattattcag gaacaaccct ctttcgtagc
     4621 agaaacccgt gcggctgaca aaaatacaga tgctgtttta ccttggctgg cgaaagatat
     4681 tgctgtcgtt tttccgcccg aagttgtaca caccacgtta agtcatcgcc gctttcccgg
     4741 agttcctgtt cagcaagcag acaaattgac ccaattacgt cgcttagcct gtagcgtgtc
     4801 gcaacgggat aataaaacag ccacatttga cttcagcacc tgttctttag aatggcaaaa
     4861 caccgtcgcc caagcgatca gtcagatcga cggcctgaaa acaacacagc taccatcacc
     4921 agtaatggct gtactcacgg cacttgaaat gaaaccagga aggcggtctg gaatatgcta
     4981 ctgatgtaat aatacacctt caacagattg atattaaatg ggattatgcg aataatgtca
     5041 ttattattct gccgtctggc attgcacctg actacttgga gcaatattcc agatttgaat
     5101 tacgcctacg taaacattta tcactggcgg aagagtctct ctggcaaaaa tgtgcacaaa
     5161 aacttattgc cgcaattcca catattccag aatggcggca accattaatt gctttgttat
     5221 tacccgaaaa accagaaatt gcacatgaaa ttgcccagcg tctactgggg caaaaaaaat
     5281 taccctcgct tgagtggtta aaaatagtgg cgactgatga gcacattctt gcctcattag
     5341 aaaaatatca cgagccatat gccatttttg atgattacta ttgtggtgcg atatggtcag
     5401 ccaccgtatt acaggagcaa ggtgttgcag ccctgccccg atttgctccc tatgccgcaa
     5461 gtgactactg cgccgatgtg ttgcgtcata tcaatcatcc gttcgcatta acactgctta
     5521 tacgtgtagc cgggcatact aaacgctgtc acgatcgaat gacgaaagcc tgcgctgcgt
     5581 tcccacacgc agcactggca gcactggcgg aacttctagt gcaaaaagaa gagaatagtt
     5641 ggcgcattat gctaatgacg atgtttatct cgcaaccagc actggcagaa caggtcattc
     5701 cctggctttc gacacccgca gttgcagtgc tgaaatcatg ccagcaccaa ctgacacagc
     5761 cctcaaacca tgccagcgcc gatctactgc cagccatagt agtctcccca ccatggattt
     5821 cgaaaaagaa aaaatcgccg attccggtgc tggatttagc gccattaggc atcgagccaa
     5881 tctgttatct gacagaagaa atcagtaatc aacttttggc gaaatatatc tggtattcaa
     5941 aacacatcac ggttagccat gaagaaagta ctaccaacct gttggcaagg atgggttttc
     6001 aacgacggat cgctggtaca tatattaaag ctcccgaagc ggtagttgag gcatggctaa
     6061 atgaagatta ttcaacctta ctaagtgaat ttaaggtgtt tcattcacct accggacatt
     6121 attggcagtt ggggattttg acaacattgc cgctggaggc tggagaaagc agtaaaagca
     6181 tggaatgccc ttactctatc tccacatacc gataccgaat acgccatgtt acattttgga
     6241 ctcaaaggtt tacctggttt agttaactca attacacgct atccgcagga agccttgccc
     6301 atcacgaatt acttcgcagc gagtgagctg gctcctgccg tcgcccgtgc cttcaacaaa
     6361 ctcaaaacgc tacgcaaaaa cgcccgcacc tggctgttga aatacccgga acatgccctt
     6421 accggcctgc tgcctgcggc gctcggcaaa gccggtgaag cacaggataa cgcccgcgct
     6481 gccttgcgta tgcttaccga aaacggtcat cagccattac tgcaagaaat cgcccggcgt
     6541 tataaccagt cggaagtaac agacgcggtg aacgctctgc ttgcgctcga tcccttagat
     6601 aatcacccga caaaaatccc cactcttccg accttttatc agccatcgat ctggacgcgc
     6661 ccggtattaa aagcaaacgc ccaatcactg ccagataacg ccctcctcca cctcggcgaa
     6721 atgctccgct tccctcagga agaggctctg tatccgggat tattgcaggt gaaagacgcc
     6781 tgtaccgctg actctctggc tgaatttgcc taggatctgt ttaccgcctg gcagaccgct
     6841 ggcgcgccgt cgaaagagag ttgggcgttc actgcgttag gcgttctcgg taacgatgac
     6901 accgcccgca aactgacgcc attaatacgc gcctggcctg gtgaatccca gcataaacgc
     6961 gccaccgttg ggttggatat tctcgccgct atcggtagtg atatcgccct gatgcagctt
     7021 aacggcattg ctcagaaact gaaattcaaa gcattacagg agcgggcaaa agaaaagatt
     7081 gccgacattg ccgagagccg cgaactcacg gtagcggagc ttgaagatcg gttagcaccg
     7141 gatctcggtc tggatgataa cggttcgttg ctgctggatt ttggcccacg ccagttcacc
     7201 gtcagctttg atgaaacctt aaaaccgttt gtgcgtgatg tttccggcag ccgcctgaaa
     7261 gacctgccaa aaccaaacaa aagcgatgat gagtcacagg cgaacgatgc tgttaaccgc
     7321 tacaaattgc tgaaaaaaga tgcgcgtacc gtcgccgccc agcaggtagc aaggcttgaa
     7381 tccgccatgt gcctgcgtcg ccgctggtcg ccggaaaact tccaactctt cctggttgtg
     7441 catccgctgg ttcgccactt aacccgccgt ctgatttggg gcgtttatag cgccgacaac
     7501 cagctactgg cctgtttccg ggtagcggaa gataacagct acagcaccgc tgacgatgat
     7561 cttttcaccc tgccggaagg cgatatctct atcggcattc ctcacgttct ggaaatatca
     7621 ccgacggatg ctgtcgcctt tggtcagctt tttgccgact acgaactgct accaccgttc
     7681 cgtcagcttg accgtaacag ttacgccctg acagaagccg agcgcaacgc cagcgaactg
     7741 acccgctggg caggcagaaa atgcccgagc ggccgggtca tggggctggc aaataaaggc
     7801 tggataaagg gcgaaccaca ggatggaggc tggatgatca aacctctcgg tcgctggtcg
     7861 ttaatcatgg aaatcgacga aggttttgcg gtgggtatgt caccagccga acttagcgct
     7921 gagcagctct taagcaagct gtggctatgg gaaggcaaag cggaaagcta tggctggggg
     7981 agtaattcaa cacaggaagc gcagttctcc gtactcgatg ccatcaccgc cagcgagcta
     8041 attaacgata ttgaagccct gtttgaataa ggaaagagca tggacaagga attaccgtgg
     8101 ctggcggata acgcccaact ggaactgaaa tataaaaaag gcaaaacacc actcagtcat
     8161 cgcaattggc cgggcgaacc agtgcctgtt atcactgaaa gcatcattca gacactgggt
     8221 gatgaattgc tacaaaaagc tgagaagaaa aaaacattgt ctggcgttat gaaaattttt
     8281 caccggagtg gcagtccgcc attacgcagg ccatcaactt gatcggcgaa cacaaaccct
     8341 cagtcccggc ccgcacaatg gcggcactag cctttatcgc gcaaaatgac aaccaacagt
     8401 tgctcgacga aatcgtccaa caagaggggc tagaatatgc gactgaggtg gtgattgcac
     8461 gccagtttat tgcacggtgt tatgagagtg atcctctggt agtcacattg cagtatcaga
     8521 acgaggacta tggctatggt tatcgctcag aaacctataa cgaattcgat ctccgactgc
     8581 gtaaacacct ctctctggca gaggaaagct gctggcagcg ttgcgccgac aaactcattg
     8641 ccgcactgcc aggaataacc aaagttcgcc gcccttttat tgcgctgatc ctccccgaaa
     8701 aaccagaaat cgccaatgag ttggtaagcc ttgaatgccc gcggactcat tttcattcta
     8761 aggagtggtt aaaagttgtt gctactgacc ccaaagcggt gagaaaactc gaacgctact
     8821 ggagccagga tatatttagc gatcgagaag ccagctacat gtcgcatgaa aaccacttcg
     8881 gctacgcggc ctgcgctgcc cttttgcgcg aacaaggact ggcagtcgtt ccgcgcctcg
     8941 cgatatatgc ccataaagaa gattgcggca gtctgctggt acaaattaac catccgcaag
     9001 tcatccgcac cttgctgtta gttgctgata aaaacaaacc cagcctgcaa cgtgtggcta
     9061 aatacagtaa aaacttcccc catgcgacac tcgccgcact ggcagaactg ctggcgttaa
     9121 aagaaccacc agcccgccca ggttatccaa tcatcgaaga caaaaagctg cctgcacagc
     9181 aaaaagctcg agatgaatac tggcgtacgc tgttacaaac gctgatggca tcgcagccac
     9241 aactggcagc agaagtgatg ccgtggttaa gtactcaagc ccgggcagtg ctgaatagtt
     9301 atttatcggc accgcccaaa ccagttattg atagcaccga taacagccaa atgcctgaaa
     9361 tcctcgtttc accaccatgc cgtgggagaa agaaaaagac ttccatacgt cacttttcgc
     9421 tgcctgatct tacgcttgca ccgcaaacgt acaggtcgcc gaataaacac tggacgccgg
     9481 aacagcaaac tgcaattgac cattttagcg ccatgtcttt tgacgaacgt cttgctacga
     9541 gaggagcgga tatcttcttg cgggaattag gttttgacgt tcatagcctg gaatttaata
     9601 agcgtcttct taatggacag ttggatgcct ttgatgctca gcaactgcaa gaatacacat
     9661 caataggact attgcgtact tatcacgcgc atcaggccaa atctaaacag taccgggaag
     9721 acgccaccaa tgcattactg gcacaagata gcattgcgtt gatgggaggg tggcgaactt
     9781 ttcagcacgg attttactat ggtaatgaac agcgttgggg gatttggaat ctgtacctta
     9841 tcgcgcagat gccgcgcgaa atggcggtat cgtgctggca acaaattgtc gaggctgatt
     9901 ttcattacac cggggtggaa tatttgttaa gtgtgctggg taccgatgct ttgcctggat
     9961 taaacaccgc ctttgcccga catccaaaag agatatttcc gctgctgatt catttcggcg
    10021 caacagaact ggccctgccc attgcccgcg tctggcacct ttttgcgggc cagcgtaatc
    10081 tggctcgcca gtggatttta caatggccgg aacatacggc tactgcactt atccctcttg
    10141 tcttcaccaa atctagcgat aaaagcgaag ctgcattact tgccctgcgt ttactttacg
    10201 aacatgggca tggcgaattg ctacaaactg tagccaatcg ttggcagcgt acagatctat
    10261 ggcctgccct ggaacaatta cttaaacaga gtccaataga gatttacccg gcacgcattc
    10321 caaaagcccc tgatttctgg catccgcaaa tgtggtccag gccgcgcctt atcactaata
    10381 atcaacctgt taccgatgac gctctggaaa ttatcggcga aatgctgcgc tttacccagg
    10441 ggggacgttt ttatagcggg ctggaacaac tgaaaacgtt ctgccagcca caaacgctgg
    10501 cagcttttgc ctgggatctc ttcactgcgt ggcaacaagc tggtgccccc gcaaaagaca
    10561 actgggcatt tctggcgtta agtctctttg gtgacgaaag cacggcacgg gatctaacga
    10621 cacatatcct cgcctggcca caagaaggca aatctgcccg tgcggtcagt ggcctgaaca
    10681 tccttaccct gatgaataat gatatggcgc tgatgcagct gcatcatata tcgcaacggg
    10741 cgaaatcccg ccccttacgt gataacgcgg cggaatttct tcaggtagtc gcagaaaatc
    10801 gcgggctaag ccaggaagag ttagcggaca gattagtccc aacactgggc cttaatgatc
    10861 cgcaggcgtt gagttttgat tttggtcccc ggcagtttac cgttcgcttc gatgaaaacc
    10921 tcaacccggt tatctttgat cagcaaaacg ttcgccagaa aagtgttccc cggttgcgcg
    10981 ccgatgacga tcaactgaaa gcgcccgagg cactggcccg actaaaaggg ttaaaaaaag
    11041 atgctactca ggtgagcaaa aacctgctcc cgcgtcttga agctgcccta cgtaccaccc
    11101 gacgctggtc gctggcagat tttcattctc tgtttgttaa tcatcccttt acccgtctgg
    11161 t
//
LOCUS       DBKEKI010000127        11158 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.128, whole genome shotgun sequence.
ACCESSION   DBKEKI010000127 DBKEKI010000000
VERSION     DBKEKI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.128"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..711)
                     /locus_tag="ACUQPX_003315"
     CDS             complement(<1..711)
                     /locus_tag="ACUQPX_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9057912.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVVFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNIFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFT"
     gene            complement(922..1374)
                     /locus_tag="ACUQPX_003316"
     CDS             complement(922..1374)
                     /locus_tag="ACUQPX_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HHV9057913.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(1427..2761)
                     /gene="adeQ"
                     /locus_tag="ACUQPX_003317"
                     /gene_synonym="yicO"
     CDS             complement(1427..2761)
                     /gene="adeQ"
                     /locus_tag="ACUQPX_003317"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HHV9057914.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            2936..4688
                     /gene="adeD"
                     /locus_tag="ACUQPX_003318"
                     /pseudo
     CDS             2936..4688
                     /gene="adeD"
                     /locus_tag="ACUQPX_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
     gene            complement(4734..6125)
                     /gene="uhpT"
                     /locus_tag="ACUQPX_003319"
     CDS             complement(4734..6125)
                     /gene="uhpT"
                     /locus_tag="ACUQPX_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HHV9057915.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(6263..7582)
                     /gene="uhpC"
                     /locus_tag="ACUQPX_003320"
     CDS             complement(6263..7582)
                     /gene="uhpC"
                     /locus_tag="ACUQPX_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HHV9057916.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(7592..9094)
                     /gene="uhpB"
                     /locus_tag="ACUQPX_003321"
     CDS             complement(7592..9094)
                     /gene="uhpB"
                     /locus_tag="ACUQPX_003321"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HHV9057917.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(9094..9684)
                     /gene="uhpA"
                     /locus_tag="ACUQPX_003322"
     CDS             complement(9094..9684)
                     /gene="uhpA"
                     /locus_tag="ACUQPX_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HHV9057918.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(9758..10048)
                     /gene="ilvN"
                     /locus_tag="ACUQPX_003323"
     CDS             complement(9758..10048)
                     /gene="ilvN"
                     /locus_tag="ACUQPX_003323"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HHV9057919.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(10052..>11158)
                     /locus_tag="ACUQPX_003324"
     CDS             complement(10052..>11158)
                     /locus_tag="ACUQPX_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HHV9057920.1"
                     /translation="AFSEESIRDAATMINAAKRPVLYLGGGVINAPARVRELAEKAQL
                     PTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQ
                     FCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQLIPQVEAQPRAEWHQLVADLQ
                     REFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQAYPLNRPRQWLT
                     SGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMN
                     NEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFGLETCDLNNEADPQAALQEII
                     NRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
BASE COUNT         2934 a         3162 c         2780 g         2282 t
ORIGIN      
        1 cgtgaagaaa gcaaactgcc cggcgaaaga catgaagatg gcgatcatcc ctgccatcac
       61 acccggacgt tgtaataagc ggaaaatatt ttgtttctga tgcgagggtt cgcctggcag
      121 tgaaggcaat gatttgataa tccagaaaat acacagcacg cccatcaccg ccgccgcatt
      181 aaagacattg cgccaaccga taagctcgcc taaaaaactg cccaacggcg cggcaatcac
      241 cagcgcaata gaaaccgcgc cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg
      301 taccagacgc atggtcagcg acgccgacat cgcccagaac ccgcccagcg ccagccccag
      361 acaggcacga ccgattaaaa gcaaactgaa tgagttagca aaggaaacca gcaagcagga
      421 gagcgtcagc aaaacggcaa acaaaataac aacgtagcgg cggtcagtgg cctgaattgt
      481 ctgggtaata aacaaactgg caaacattgc cacaaaggcg gtcacggtca ccgattgccc
      541 ggcaacccct tccgaaatgc ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa
      601 aaactcaacg ataatcagac aggcgacaca aaacaccacc gagaaaacgg ctgaccagtt
      661 cggtcgggtg atggcatccg cgccgcggtt ttcggcaata aattcactca tggtgttacc
      721 cgtggcatgt ttcaggggaa aagccgacca ctttaacatt gaaatgtgtg acgcatttaa
      781 cgtttttgca acttttacgg tgttgcttga gctggagtta agatcgaaac ggaacagggc
      841 aaagattacc ggatgcggcg tgaacgtctt atccacccga caggtcaggc gcggtctgta
      901 ggctgataag acgaaacggt ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa
      961 cgcgttatag cgcatctgaa aagcattaat atcctgcatt tcaggttcag tttcacgcag
     1021 aaaacccagc gccagcctga cctgagcatc ggtaatcggc gcggaaaggc gggctttttg
     1081 taaaatccgg tgccattgca gcaggttctg ttcgctatca tcgacaatga caatttgcca
     1141 gatgagcaac acctgcgcgg cgttttgcag gtgcgactca tccgggcgat gcaaataacg
     1201 taaaaattcg tcaccggctg actttcccat ttgatcgacc atcgactcca cgggtacgac
     1261 atcgatattc agccgatcgc tgagacggcg aatcgctttt ctggccccgg atgtcagcac
     1321 ccgaaaacca accacaaaca ctaccgccag cgtggcgagc attatccaga tcatgagttc
     1381 tcctgcgtaa tcagaactca tgatatctgg aaaccctcgc cagttactaa tccaccagaa
     1441 taatcttcaa tgcgaacaga gctgcgacca ccaccacaca caggttcaga tcgcgccagc
     1501 gcccggtgca cactttcatg atgcagtacg acataaagcc gagtgcaatc ccttcggtga
     1561 tcgagaaagt aaagggcatc atcaccgtgg taataaacgc aggcaccgat tcggtaaaat
     1621 catcccagtt aacgcgcgcc aggctcgaag tcatcagcac gccaacaaag attaacgctc
     1681 cagcggttgc gtaaggagga accatcgcca ccagcggtga gaagaacata actaacagga
     1741 acataacgcc gaccacgaca gccgttagcc ccgtgcgacc accgactgcc acaccagaag
     1801 tactttcaat ataggcagta acagacgagg taccgataaa tgcgcctgcc accgaactga
     1861 cgctatcaac atacagcgcc ttattcatat tggggaattt accgttgcta tctattaagc
     1921 ccgctttatc agttacacca attaatgttc ctgatgaatc aaataggttg atcagcataa
     1981 aggagaaaat gataccggcg agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa
     2041 tgacgccgct aatatcaggc ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa
     2101 ataatccaca gcaagacgtc accacaatag aaaccagcac cgcggcatga aaatgacgtg
     2161 atgacaacac ggttataata aaaaacccta aaatacctaa caacacgccg tgagaactta
     2221 aatcgccaat catcaccagc gtgtctttat tggcgacaat aacaccagtg ttttttaatc
     2281 ccattaaggc aataaataat ccgattccac tggtgatacc aatacgtaaa cttaatggaa
     2341 tgttggagat catccagtac cggatacgaa acagcgtgag caaaaatagc ccaattgcgc
     2401 cccagaatat cgcgcccatc ccggtctgcc aggagatccc catcgccccc acgaccacga
     2461 acgcaaagaa ggcgttcagc cccattgccg gagccagcgc cacgggtaag ttagcaaata
     2521 tccccatcgc aatactgccg ataccggcaa tcaaacaggt ggtaacaaac accactttcg
     2581 ggtccatttg tgccgcgccg aggatttgcg ggttcacaaa aacaatgtac accatggtta
     2641 aaaaagtggt catccccgca atcaattctg tgcggacggt ggtcccatgc tgaggtagtt
     2701 taaataatcg cgaaagcgtc cctgattcat tactcacgta atcggtattg tcattattca
     2761 tttttttgtc cattggagga gatttaatcc cttgcttttg taaaacaaat gacaacaacg
     2821 gttcagtgat aattatcaca tttcaattgc acattaatag atattcttta ataatctcgc
     2881 gacgtttctt tatgataaat aataatcaaa ttgataaaat caaaatgaga aaaatatgaa
     2941 taattccatt aaccataaat ttcatcacat tagccgggct gaataccagg aattgttagc
     3001 cgtttcccgt ggcgacgctg ttgccgatta tattattgat aatgtctcta ttctcgacct
     3061 gatcaatgcc ggagaaattt ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt
     3121 tggcgcagaa tacgctgatg ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc
     3181 ggtgccaggg tttattgatg ctcacctgca tattgaatcc agcatgatga cgccggtcac
     3241 ttttgaaacc gctaccctgc cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat
     3301 cgtcaacgtg atgggagaag ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag
     3361 gcaaaaccag tacttacagg tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa
     3421 cggtgccagt tttacccttg aacagatgct cgcctggcgg gaccatccgc aggttaccgg
     3481 ccttgcagaa atgatggact accctggcgt aattaacggg cagaatgcgc tgctcgataa
     3541 actcgatgca tttcgctacc tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga
     3601 acttaacgcc tatattgctg cgggtattga aaactgccac gaaagttatc agctggaaga
     3661 aggacgccgg aaattacaac tcggcatgtc gttgatgatc cgcgaagggt ccgctgcccg
     3721 caatctcaac gcactggcac cgttgatcaa cgaatttaac agcccgcaat gcatgctctg
     3781 taccgatgac cgtaacccgt gggagatcgc ccatgaaggg catatcgatg ccttaattcg
     3841 ccgcctgatc gaacaacaca atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc
     3901 gacggcgcgc cactttggtc tgaatcacct cggcttactg gcacccggca agcaggccga
     3961 tatcgtcctg ttgagcgatg cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga
     4021 accgattgat gcgcaaacct tacaggcgga agagtcggcg agactggcgc tgtctgcccc
     4081 gccatatggc aataccattg cccgccagcg actttgccct gcaattcacg cccggaaaac
     4141 gctatcgggt cattgacgtc atccataacg aattgattac gcactcccgc tccagcgtct
     4201 acagcgaaaa tggttttgat cgcgatgatg tgtgctttat tgccgtactt gagcgttacg
     4261 ggcaacggct ggctccggcc tgtggtttgc tcggcggctt tggcctgaat gaaggcgcgc
     4321 tggctgcgac agtcagccat gacagccata atattgtggt gatcggtcgc agtgccgaag
     4381 agatggcgct ggcggtcaat caggtgattc aggatggcgg cgggctgtgc gtggtacgta
     4441 acggccaggt acaaagtcat ctgccgttac ccattgccgg gctgatgagc accgacacgg
     4501 cgcagtcgct ggcggaacaa attgacgcct tgaaagccgc cgcccgtgaa tgcggtccgt
     4561 tacccgatga gccgtttatt cagatggcgt ttctttctct gccagtgatc cccgcgctaa
     4621 aactaaccag tcaggggcta tttgatggcg agaagtttgc cttcactacg ctggaagtca
     4681 cggaataata aaaaaagccc ggcgtcatgc cgggcaaaag tcaccagtta cgtttatgcc
     4741 actgtcaact gctgaatttt tttctcgcgg cggattttgc gttcttccat taccgccact
     4801 atcgccatca ggcagataca accaatcgcg gcgatatcca gcgcggcgaa ggtgcctgcc
     4861 cagccggtaa ggccgaatac cggcgtccca tcggcaatca ttcccagacc taacttggca
     4921 aagctgtcac caatcaggta agcaaaggtg cctttaatac catcggcagc gccaatcgct
     4981 tttttaggta caaagccaac agcagccaca ccaatcaaca attgcgggcc aaagaccaag
     5041 aaacccaacg caaagagaga agccagatag atatattcgt tactggcatg ttgatacaca
     5101 ccgagcgtgg cgataatcag cgccagcgcg atgcaggcca ccaggccacg gcgaccgttc
     5161 gccaggtcag agagccagcc ccacagcagc gtaccgacca gcgcaccagc ttcaaacagc
     5221 gtaaagccct gaatcgccac cgctttagag agtttcagtt cctggaacgc gtatacggtt
     5281 gaccactggt cgataccaat acgtaccaca tagaggaaaa tgttggcgaa gcacagcagc
     5341 cagatcactt tgtttttcag cacatactca acaaagatct gccacttggt catatcggta
     5401 gattctgtct ctttgtcctc ttcgctgatc tcctcgccga acagttcttc agctttgccg
     5461 aggccataag attccgggga gtcgctgccg taacgcaggc cgataaaacc gacaatcagc
     5521 gcgataatcg acgggaagat aaacatgccg atgacatggc catcgaacag gtaatttgcc
     5581 ccgaacagcg ccacacctgc tgcgcctgca ccgccaaggt tgtgagaaat attccagaaa
     5641 ccgaggaatg tcccgcgttt acgacgcggc gtccatttgg tgatggtgga gtaactgcac
     5701 gaaccgccgg tactctggaa aaagccgctt aaggcgtaga aggcaatcat caggaacagg
     5761 ctaaccgagc cgctgcccat actggcactg aagcccagca tacaaatggc agagaggata
     5821 agcatgaacg gcaggaattg tttggtgttt ttgccgtcgg cgtagtatga aaccaacgtt
     5881 ttacccacgc cataagtgat ggagaaaccc aggccgatca tccccagctg cgtcatgctc
     5941 aacccgtagg tcgaaatcat atcgttctgc gcgatgttaa agttcttgcg aatcaggtac
     6001 atcgtcaggt agccgataaa gaccaccagg taggattgca tgaacggttt gaaccacatt
     6061 ttgcgccgca cttcgagcgg aaggtccagg gtcggcttgc gaacctggtt taggaaagcc
     6121 agcatgggtt actcctgaaa tgaatacctg cgtgaggcat gcattgtaaa aatcagcagc
     6181 aaatcaggcc tgagatagcg tccaagaaaa acctggaaaa tttcttagtt ttgccggata
     6241 tgaagtgaaa aggtgagatg catcacgctt cacgcggtgt ctgggcgttc aaaaagggca
     6301 gtaacagcag tgcggaaatc ccggcggcga tagagataac cacaaaaaat ccgctccagt
     6361 gccaggtatc gagcactttc gccagcggcc aaccagcaag cgacgccccc agataagcaa
     6421 acaagccgac aaaccccgtc gccgcccctg ccgcctcttt gtgggaacac tctgccgccg
     6481 ccataccaat taacatctgc gggccaaaga caaaaaaacc aatggtgaag aagcaggttg
     6541 cctgcatcac gtagctggca aatggcatca gccacagcga gccgactgaa agcaaaattc
     6601 cggcggcgaa aatcaaattc atcggcccac ggttgccgtt aaacaatttg tccgagcccc
     6661 aaccggctac cagcgcaccg ataaatccgc ccagttcaaa catcgtcact gccgtattcg
     6721 ccgtgaccag atcgacgccc agcgtctcgg acatatacaa attgccccag tcgttgatcg
     6781 ccgcccggac cacatagacc agcacatagc aaaacgaaag cagccagata tacggattca
     6841 gcaacacata tttggtgagg atctctttac gcgtcaaccc tgccccttct tgttgttgag
     6901 caatttccag cgcgtcgtgc cgccattcac cgaccgccgg taaacctaac gcctgcgggc
     6961 gatcgcgtag ccgccagcag agaaaaatcc ccacgactat cgccatacaa ccagcaatca
     7021 tcatcccggc acgccagccg taatgcagcg cgctcgctgc catcacaatg ggaatgagtg
     7081 cgccgccgac gttatgcgcc gtgttccata atgcccacca accgccgcgc tcggtacgtg
     7141 aataccaggc cgttaacaga cgcgcacaca ccggtgaacc ccagccctgg aaaaaggcgt
     7201 tcagcaccca gagcacggca aacgcccata gcgacgtcga gaagccaaac agaatgttca
     7261 tgatgcccgt ggcaataagc cctatcccca taaaataacg ggcatttgag cgatcgctga
     7321 caatgccgga gacaaacttc gacacgccgt aagtaatgta aaacagggtc gctaacaggc
     7381 cgatatcgct acggctgagc acgccgttag caaggatttc tggtaccgcg gcgttaaaac
     7441 ttttccgcgt gaagtaaaac agcgcgtaac ccagccagat ggtcagcaga atatgccgac
     7501 gccagtagcg ataacgggca tcaatttcat gtttatcagt cattaatggc gcatcggcag
     7561 gcgctttcag aaacggcaac atcatcgaac cttagacata acgttgaggt agagaaacgc
     7621 tgacacgcgt gccgtgcaga caggaaatgt gtaatgtgcc gcccagcgcc gttacgcgct
     7681 cgcgcattcc ggtgaggcca aaaccttgtt gcccggaacc cggcggcaaa ccgctgccat
     7741 cgtcttcaat caccagcatc aaccgttcat cctgctgcca gccttgcagc gtaaccgcgc
     7801 tggcatcggc atgtttcaca atgttgttca gcccttcctg gcagacacga aacagcgtca
     7861 cgcgctggtt ttcgcttaat gctgattcat cgatacgcca ttcgagatgg ctgacaatgc
     7921 cgcgcccttc cagctccatt tcccgcatca gtgagcggat ggcctgctcc agggtgagat
     7981 catccagctg gcgcggacgt aaccgtccta acaaacggcg caccgcgtcg taaacgccca
     8041 gcgacagttg ttcgatgagc tgcccgctct gcttcacact ggcgttatct gccgccagcc
     8101 gctgaacaat gcccgcctga gtacgaatag cagtgatggt ctgaccgata tcgtcgtgca
     8161 gctcacgcgc cacgtcgcgg cgcacgctct cttcggtttc taacaaacgt tcagccagat
     8221 gctgattgcg cgccagttcc ttttgtagcg actggttaag ttcacgcaac cgctggatgc
     8281 ctgcgcccag taacaaccct gtcagacttt gcaccagcaa cgagagtaat aaatccaccg
     8341 gatgatcgcg ccaggtttga ctggcgatca gcgcgatggc gttcatcaac gtcgcaatca
     8401 gcgctccttg ccagccatag tgccaggcca gcgcgataat cggcagcgcc agacagaatg
     8461 gcgtaaagcg cgacagttcg tccggcaatc ccaactggag ccagagactg ataacaaaca
     8521 gcagcaagta ccagaccaga tgccgcccgc gccagttgat gggctgagaa accagtgacg
     8581 ggccgagcgg cagccaggtg ttattggcga gatagtgcca gaacaccaga catatcgggg
     8641 ccagcgtcag accgccagtt aaagtcagca acagcgcatt ccacgactct ttgccgtgcc
     8701 aaagccaggg cagcgactgc aacaacgccg ccgccgttaa cgccgcaccc tgtaacagca
     8761 aggtgcgcca gtcacgctga tggcgatagc gcgagatcag cgctacgggc agtaacgtca
     8821 gtaaactacc gatcatcaat aacggaaaat gggttaaacc gaccgcctgc gttagccagt
     8881 aaatcagcag ccactccgca cccagcaata cgggccagta tccgcgcggg cattgcagca
     8941 ttagccccag acgcagacca aacggaaata acagcaccgc catatcaggg cgctcaacca
     9001 gatgcaggct gatactccat aggcaaaacc atgcggcaga gaagataaaa aagcaggcaa
     9061 taacggtaat taagcgggag aacaacgtct tcatcaccag ccatcaaaca tgcggcgcgc
     9121 cagctctacg tcgttactga cgcccagttt ttccatcaga ttggcgcgat ggacgtgtac
     9181 cgttttcggt gacaagccca gttcggcggc aatctctttc accgccattc cttgcgccag
     9241 tttttccgcc acctggcgtt cacgtttggt cagcgggtcc tgacgaccgg atgccagttt
     9301 aatggcaata tccggcgtca gataacagcc gcccgtggca accgtatgca ccgcagcaat
     9361 gagttcatcc gggctacagc gtttggaaag aaagccgcgt gcccccgcgt taagcgcctg
     9421 ctcaaccagc gcaggactgt cgtgaacgga gagcataatc gtcgccatac ctttcggcag
     9481 ctggcttagc agctccagac cggagatatc gggcatggag atatcgcaaa tacacacctg
     9541 cacaccgcgc cccggcagcc ccgccagcgc ctcgcgcccc gaaccaaact cggcaactac
     9601 ctgcaaatca ggttccagcc ccagcagctg cgcaaagccg gagcggacga tgaggtgatc
     9661 gtctataagg gcaacggtga tcatggtctt gtcctggcgg gtaaaaaaaa cgcgcttacc
     9721 ttaacgataa gcgcgatgtt gttcaagcct tgagcggtta ctgaaaaaac accgcgatct
     9781 tgttaaacat cgtcggatcg gactgattac gctgcacttt cacgacatct tccagcttat
     9841 cgatttggct tatcatctgc tccagacgct ggtcgtcatt gaccagtagc cagatatggc
     9901 ttttgtcgct gtcctgaatc ggcagacaaa gaatgccttc aacgttaaaa gcgcggcggg
     9961 caaaaaggcc acaaacgtgg gtcattacgc ccggatggtt gcgaacggtg agctccagaa
    10021 ttacgttgtt atgagttgtg ttttgcatgg cttattcccc caccatttca gtattcgccg
    10081 cacctggcgg caccatcggg taaacttttt cttcggcatc aatgcgcaca tggatcagcg
    10141 ccgggccagg gcgattgatg atttcctgca atgcagcctg cggatcggct tcgttattca
    10201 aatcacaggt ttcgaggccg aatccggcgg caatctgcat aaagttgatt ttgcccggat
    10261 aggtggcggc aaaaacgcct tgctcgtaga acagactctg ttgctgatgc accagcccca
    10321 gcgcttcgtt gttcatcaga atgattttga catccagctg attttcactg gcggtcgcca
    10381 tctcctgaat attcatcatc aggctgccgt cgccggagaa acacaacact ttgcgatccg
    10441 ggttcgccag cgccgcgcca atcgccgcag gcaggccaaa acccatcgtg cccagcccac
    10501 cggaggtcag ccactggcgt gggcgattga gcggataagc ttgcgcggtc cacatctgat
    10561 gctgacccac atcggtggtg ataatcgcat tgtcatcgac acaggcggca acggcgttga
    10621 tcaggccgta atggcttaat ggatcgcacg ctttcgggat tggacacggg aattcgcgct
    10681 gcaaatccgc taccaactgg tgccactctg cacgcggttg cgcttccacc tgcgggatca
    10741 attgcgccag cacgtcatca acatccgcct gaatcgccac atgcggctgc ttgattttac
    10801 ccagctctgc gcgatcgata tcgacatgaa taattttggc attcggacag aactgctcgg
    10861 ttttgccaat cgcccggtca tcaaaacgcg caccgagcac aatcaacaaa tccgcctcct
    10921 gcaaaatata gttggtactg cgcacgccgt gcatccccag catacccagc gacaacggat
    10981 gcgcttttgg cagcatgccc agcgccatta aagtcatggt ggtaggcagt tgcgctttct
    11041 ccgccagttc acgcacccgc gcaggcgcat tgatcacacc accacccaga taaagcaccg
    11101 ggcgtttggc agcgttaatc attgtagctg cgtcacgaat gctttcttcg ctaaaggc
//
LOCUS       DBKEKI010000128        11013 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.129, whole genome shotgun sequence.
ACCESSION   DBKEKI010000128 DBKEKI010000000
VERSION     DBKEKI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.129"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            390..1604
                     /gene="manD"
                     /locus_tag="ACUQPX_003325"
     CDS             390..1604
                     /gene="manD"
                     /locus_tag="ACUQPX_003325"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HHV9057921.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLLNW"
     gene            1671..2954
                     /locus_tag="ACUQPX_003326"
     CDS             1671..2954
                     /locus_tag="ACUQPX_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9057922.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3206..4123
                     /gene="oxyR"
                     /locus_tag="ACUQPX_003327"
     CDS             3206..4123
                     /gene="oxyR"
                     /locus_tag="ACUQPX_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HHV9057923.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(4106..5506)
                     /gene="sthA"
                     /locus_tag="ACUQPX_003328"
     CDS             complement(4106..5506)
                     /gene="sthA"
                     /locus_tag="ACUQPX_003328"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HHV9057924.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5827..6477
                     /gene="fabR"
                     /locus_tag="ACUQPX_003329"
     CDS             5827..6477
                     /gene="fabR"
                     /locus_tag="ACUQPX_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HHV9057925.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6477..6836
                     /locus_tag="ACUQPX_003330"
     CDS             6477..6836
                     /locus_tag="ACUQPX_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HHV9057926.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6876..7976)
                     /gene="trmA"
                     /locus_tag="ACUQPX_003331"
     CDS             complement(6876..7976)
                     /gene="trmA"
                     /locus_tag="ACUQPX_003331"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HHV9057927.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      8090..8279
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8345..10189
                     /gene="btuB"
                     /locus_tag="ACUQPX_003332"
     CDS             8345..10189
                     /gene="btuB"
                     /locus_tag="ACUQPX_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HHV9057928.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            10134..10991
                     /gene="murI"
                     /locus_tag="ACUQPX_003333"
     CDS             10134..10991
                     /gene="murI"
                     /locus_tag="ACUQPX_003333"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HHV9057929.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLLA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2534 a         2595 c         2922 g         2962 t
ORIGIN      
        1 tctggtgacc ttaaatttaa cgcccaacca gaaccattat ttgtcacatt cctttttatt
       61 attgtgtctt ctttacctcc actctcacca tgaaaataat attttcaaat acatcaagca
      121 atacacattt taattaattg aatataatta atttctattt ctgtgctcat tttcaatgat
      181 taatggagtt tgttatgtga ttatttaacg tgttgtttta acaataatgg ttaattattc
      241 aggtggtttt tgagggtatt ttttctcctc ggtggaaatt ttttgttttt aatgtgaaca
      301 cggttgaata atcgcttttt ctctcttgta tggtagtagg cgtaaaatta agtttgaaac
      361 gtctatttac aacacaggag attgcaataa tgaaaattat tgctgcagat gtttttgtta
      421 cttgcccagg gcgtaatttc gttacgctta aaataaccac tgaaagcgga ctttgtggat
      481 taggagatgc cacgttaaat ggtcgcgaat tgtcggtcgc ttcttatctg aaagatcatc
      541 tttgtccaca actaattggc cgcgatgcca gccgtatcga agatatctgg caattctttt
      601 ataaaggtgc ttactggcga cgtggccccg tcacgatgtc agccatttct gccattgata
      661 tggcgttgtg ggatatcaaa gctaaagcgg caaatatgcc attgtatcaa ctgcttggtg
      721 gtgcttctcg tgaaggcgtg atggtttatt gccacaccac cgggcgtacg attgatgaag
      781 tccttgaaga ctatgcaaag caccagcaga tgggctttaa ggcgattcgt gtgcagtgcg
      841 gtgtaccggg tatgcaaacc acttatggcc tggcgaaggg caaaggactg gcgtatgaac
      901 cggcaaccaa aggcctgtgg ccagaagagc agttgtggtc ttcagaaaag tatctcgatt
      961 ttacgccgaa gttattcgaa gcggtgcgta ataagttcgg ttttaacgaa catctgttgc
     1021 atgacatgca ccatcgcctg acccccatcg aagcggcgcg ctttgggaag agcattgagg
     1081 attaccgcct gttctggatg gaagatccga cgcctgccga gaaccaggaa tgttttcgtc
     1141 tgattcgcca gcataccgtg acgccgattg cggtcggtga agtgtttaac agtatctggg
     1201 actgcaagca gttaatcgaa gagcagctga ttgactatat tcgcacgacg atcacccatg
     1261 caggcggcat taccgggatg cggcggattg ccgattttgc atcgctttat caggtacgta
     1321 ccggttcgca tggcccttca gatttatcgc ctgtttgtca tgcggcggcg ctgcattttg
     1381 acctatgggt accaaatttt ggcgttcagg agtacatggg ctattccgaa caaatgcttg
     1441 aagttttccc gcacagctgg cgcttcgagg aaggttatat gcacccaggc gatgaaccgg
     1501 ggctgggtat ttctttcgat gagaaactgg cggcgaaata tccgtatgac ccggcgtatc
     1561 tccccgtcgc acgactggaa gatggcacat tgttgaactg gtaataacgg ttcgcttaat
     1621 tattttctga acagtcatca aatcgattaa ttccgctcaa ggagctaaaa atggacgttg
     1681 atgtgtcaac cagtgttgcg ggtaataaac cgcaacggat tcgtcgtatt cagacagtga
     1741 ccctggtttt attatttatg gcggggatcg ttaattttct cgaccgttcg tcattgagcg
     1801 tggcaggcga agcgattcgt ggcgagttag gattatcggc gacagagttt ggtgttttgc
     1861 tttctgcatt ttctctgtct tatggttttt cacaactacc ttccggtatt ttgctagacc
     1921 gctttggccc acgaatagtt ttaggcgcag gcttaatatt ctggtcatta atgcaggcat
     1981 taacaggaat ggttaattct tttagccact ttattttaat gcgtatcggt ctgggtattg
     2041 gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactggtat gcacaaaaag
     2101 aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag gctatcgcgc
     2161 ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc gttatcatcg
     2221 gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac cgggcgcagt
     2281 ttgtcctgac tgacgaagaa cggacgtatc tctccgcgcc ggttaaaccg cgtccacagc
     2341 tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg atgattttag
     2401 gtttctctgg cgtcaattat accggttggc tctacattgc gtggttaccc ggttatttgc
     2461 aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt cctttcctcg
     2521 cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg aaaaaaggct
     2581 acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg tcggcattag
     2641 gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt atctcaatgg
     2701 cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag gtgatggtgt
     2761 cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt gtttttgctt
     2821 cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt aatctggcgc
     2881 tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt attgtcaaag
     2941 atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag aataaaaaaa
     3001 agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag ttgagaaact
     3061 ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc tgtgagcaat
     3121 tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta tcgccatgaa
     3181 ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc tggtggcatt
     3241 ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc agccgacgct
     3301 tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg agcggaccag
     3361 ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc gtaccgtgct
     3421 gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggcgagacga tgtccggacc
     3481 gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata ttatcccgat
     3541 gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga cccaccagtt
     3601 actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg tgaaagagag
     3661 cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta tctatgaaga
     3721 tcacccgtgg gcgaaccgcg aatgcgtacc gatggccgat ctggcagggg aaaaactgct
     3781 gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct ttgaagccgg
     3841 ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca acatggtggc
     3901 ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc gcaaacgcga
     3961 tggggttgtt tatctgccat gcattaagcc ggaaccacgc cgcactattg gcctggttta
     4021 tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca tccgcgcaag
     4081 aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt aacgcagcta
     4141 cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag tactcaatag
     4201 tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt tcggcagcgc
     4261 gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac aaaattttca
     4321 gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac tgggcgcggc
     4381 ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc acagagctga
     4441 tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg gtggcttcgc
     4501 cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat gccaggctcg
     4561 gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga tacatgctgt
     4621 tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac gccagcgaat
     4681 cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt tttttacccg
     4741 acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac tcttcgttgt
     4801 gacgaatcac tacgccactg ttccagaagt gataggagag agaatctgac atctcttgat
     4861 cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca tccataccgc
     4921 ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt acatggcgcg
     4981 gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg gtgaaatcaa
     5041 catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca gcggttagtg
     5101 tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca aagcgagcgt
     5161 tcccctgcaa tatttcacag tgattacgtt cgtaaaatcc ctgacgcatg cgcgtttgtt
     5221 gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg agcagtcggg
     5281 aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg gcgtgacgga
     5341 gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt tgataacgct
     5401 cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct tcgccgccgg
     5461 ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta gggcttacct
     5521 gttcttatac ataaaagcaa cagaatggta acattctatc gcgggtaagc caattgatcc
     5581 ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct tacttttgcc
     5641 ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag gtttccgctg
     5701 tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt cacaatactg
     5761 gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg caaggattca
     5821 gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc gctggtggaa
     5881 gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt gcgtgaagtg
     5941 gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga cgtagacgaa
     6001 ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat gcgccaggcg
     6061 cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac atttatggag
     6121 ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc cggcacctcc
     6181 gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga acttgcggac
     6241 tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc cgaagcaatg
     6301 gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga acaacgtcgg
     6361 caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc ttattactgg
     6421 tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga cgagtaatga
     6481 aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggc ttatcgatta
     6541 atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc ccgattattt
     6601 ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc atgtcggtag
     6661 gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac agcacggtag
     6721 ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc tccgtcatta
     6781 tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct gagtaatcgg
     6841 tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag caatacgccg
     6901 cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc gaccttgtgc
     6961 gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga gatatacaga
     7021 atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct gcgcggtggg
     7081 tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag gcggttaaac
     7141 tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat aatttgtacg
     7201 ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg tttggcgatt
     7261 tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt accgttgccg
     7321 cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc ccattccagc
     7381 atctgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg acgatagatc
     7441 atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag cgcgattttg
     7501 gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag tgcatcgcgc
     7561 agtgcttccg cttcctgacg ccactcatca tccagctttt tatggtacag cagggaaacc
     7621 accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg gcgcagaacg
     7681 ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag ttcactggcg
     7741 gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat gatgtgatac
     7801 aggtcatcgc catcgtgcca gatgcggaac tccgcgcgca tccggtaatg actgaccggc
     7861 gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg caaacgtacc
     7921 actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg ggtcatgatg
     7981 tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat gtccagatct
     8041 tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc tgtagcatcc
     8101 acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctggagctg acgcgcagcg
     8161 gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg aagtcatcat
     8221 ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg tcgcatctgg
     8281 ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt gtggatgctt
     8341 tacaatgatt aaaaaagctt cgctgctgac ggcgtgttcc gtcacggcat tttccgcttg
     8401 ggcacaggat accagcccgg atactctcgt cgttactgct atccgttttg aacagccgcg
     8461 cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc gctggcagtc
     8521 gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc aaaacggcgg
     8581 ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg tgctggtgtt
     8641 aattgatggc gtccgcctga atctggcggg ggtgagtggt tctgccgacc ttagccagtt
     8701 ccctattgcg cttgtccagc gtattgaata tatccgtgga ccgcgctccg ctgtttatgg
     8761 ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac ccggaacgga
     8821 aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta cgcagcaaca
     8881 actgggggat aaaacacggg taacgctgtt gggcgattat gcccatactc atggttatga
     8941 tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg gttttttaag
     9001 taaaacgctt tatggggcgc tggagcataa ctttactgat gcctggagcg gctttgttcg
     9061 cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg gttcaccgtt
     9121 gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct ataacggcga
     9181 actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca actacgatcc
     9241 ccattatggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat acaccgtcca
     9301 gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg actggcagaa
     9361 acagactacg acgccgggta caggttatgt tgaggatgga tatgatcaac gtaataccgg
     9421 catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg cagcacgcag
     9481 cgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg gttgggaatt
     9541 catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac caaatctggg
     9601 gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca aacagtggga
     9661 aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat atcgtaacga
     9721 tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg aagggaaagc
     9781 gcggattaag ggcgtcgagg cgacagccaa ttttgatacc ggaccactga cacatactgt
     9841 gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt tacgccgtgc
     9901 taaacagcag gtgaaatacc agctcgactg gcagttgtat gacttcgact ggggtattac
     9961 ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt atcaaaccgt
    10021 taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca cctctcacct
    10081 gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag tctatggcta
    10141 ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa ccacgtccca
    10201 ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag atccggcatc
    10261 tcttaccgga tctccattac atttatgctt tcgataacgt cgctttcccg tatggcgaaa
    10321 aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt caccgcggtg caagaacgtt
    10381 atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca cttcttgcat
    10441 tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa cctgctgcac
    10501 gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaacagtt aaacgttctt
    10561 atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg ctgggctcgg
    10621 cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct ctggatgcac
    10681 taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc gttgtattgg
    10741 gttgcaccca tttccctcta ctacaagaag aactgttaca agtgctgcca gagggaaccc
    10801 ggctggtgga ttctggcgca gcgattgctc gccgaacggc ctggttgtta gaacatgaag
    10861 ccccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg acgccagaag
    10921 ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa aaactggcag
    10981 ttttaggctg atttggttga atgttgcgcg gtc
//
LOCUS       DBKEKI010000129        10969 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.130, whole genome shotgun sequence.
ACCESSION   DBKEKI010000129 DBKEKI010000000
VERSION     DBKEKI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.130"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(68..1732)
                     /gene="glnS"
                     /locus_tag="ACUQPX_003334"
     CDS             complement(68..1732)
                     /gene="glnS"
                     /locus_tag="ACUQPX_003334"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HHV9057930.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1935..3881)
                     /gene="nagE"
                     /locus_tag="ACUQPX_003335"
     CDS             complement(1935..3881)
                     /gene="nagE"
                     /locus_tag="ACUQPX_003335"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HHV9057931.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAVGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISLVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4214..5014
                     /gene="nagB"
                     /locus_tag="ACUQPX_003336"
     CDS             4214..5014
                     /gene="nagB"
                     /locus_tag="ACUQPX_003336"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HHV9057932.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5074..6222
                     /gene="nagA"
                     /locus_tag="ACUQPX_003337"
     CDS             5074..6222
                     /gene="nagA"
                     /locus_tag="ACUQPX_003337"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HHV9057933.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6231..7451
                     /gene="nagC"
                     /locus_tag="ACUQPX_003338"
                     /gene_synonym="nagR"
     CDS             6231..7451
                     /gene="nagC"
                     /locus_tag="ACUQPX_003338"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HHV9057934.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7499..8251
                     /gene="nagD"
                     /locus_tag="ACUQPX_003339"
     CDS             7499..8251
                     /gene="nagD"
                     /locus_tag="ACUQPX_003339"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HHV9057935.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8648..10312
                     /gene="asnB"
                     /locus_tag="ACUQPX_003340"
     CDS             8648..10312
                     /gene="asnB"
                     /locus_tag="ACUQPX_003340"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HHV9057936.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10692..10768
                     /locus_tag="ACUQPX_003341"
     tRNA            10692..10768
                     /locus_tag="ACUQPX_003341"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10726..10728,aa:Met,seq:cat)
     gene            10777..10861
                     /locus_tag="ACUQPX_003342"
     tRNA            10777..10861
                     /locus_tag="ACUQPX_003342"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10811..10813,aa:Leu,seq:tag)
     gene            10885..10959
                     /locus_tag="ACUQPX_003343"
     tRNA            10885..10959
                     /locus_tag="ACUQPX_003343"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10917..10919,aa:Gln,seq:ttg)
BASE COUNT         2726 a         2842 c         2772 g         2629 t
ORIGIN      
        1 gaaatttatt gtctatttat tgccggatgc gacgtaaatg cctcacccgg cttggtagaa
       61 cttaaaatta ctcgcctact ttcgcccagg tatcacgcag cccaacggtg cggttaaata
      121 ccggtttttc ggccgtggaa tggcggctgt cgaggcagaa gtaaccttca cgctcaaact
      181 ggaatgcttt acccgctacc gcatctttca gcgacggttc agcaaagccc tgtttgatca
      241 ccagcgattc cgggttaatc accgacagga aatcatccgc agcacctggg ttaggcacgc
      301 tgaacagacg atcatacaaa cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt
      361 gaataacacc tttgacttta cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat
      421 aagtacagaa gatggtggtg atattacctt cggcatcttt ctccacgcgt tcagccttaa
      481 tgacgtaagc attacgcaga cgcacttctt tacccagcac cagacgtttg tactgcttgt
      541 tagcttcttc gcggaaatcg gcgcgatcga tccaaatttc accgctaaac ggcacctgac
      601 ggctccccat ttccggtttg ttcggatggt tcggcatggt gaccatttcg ccttcgccct
      661 gatagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc ggcgcatttt
      721 cgttgagatc ttcacggatg caggattcca gcgacgccat ctcaatggtg ttgtcctgct
      781 tggtcacgcc gatgcgtttg cagaactcac gaatagaagc cgcagtgtaa ccacgacgac
      841 gcagaccgga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca
      901 ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg cgcgagaatt
      961 cgtactggcg cggatgaaca ggaatggtga tgttatccag tacccagtcg tacagacgac
     1021 ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagc gcatcgctga
     1081 tgcaatgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc tggtggtgtt
     1141 cagcaaattt aatgcggtac agcaccggat cgcgcattac gataaatggc gacgccatgt
     1201 cgattttcgc acgcaggcag gctttacctt cttcaaaacc accggcacgc attttttcga
     1261 acagcgccaa gttctcttca acgctgcggt cgcggtacgg gctgttttta cccggctgcg
     1321 tcagggtacc gcggtattcg cggatctgtt ccggtgtcag ttcatcaacg tacgccaggc
     1381 ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg gaggagtaac
     1441 ggacgttacc agaccagtga aaacctaacc actctacgtc gtttttgatc gaatcgacat
     1501 actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac tgacctttat
     1561 agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata tgcagatagc
     1621 cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg cttaccactg gccagatctt
     1681 catcgatgat ctgacggata aagttagtcg ggcgggcttc tgcctcactc atcgtggatt
     1741 cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca ggctgacaac
     1801 tgttagtttt ttaaaattga taatctaaca aggattatgg ggatgattga tgaaaaaaaa
     1861 agcggcagag aagatctcca ccgcttgtca ttgttggatg cgacgctgcc gtgtcgcatc
     1921 aggcataaag cagattactt tttgatttca tacagcggtg tttgacccgc cacaacatgg
     1981 ccctgagctt taatgatcaa gccactgaaa tcgtcgatat tgctgcaaac caccaggcta
     2041 atcatcgagc gggcgttagc gttcaggtaa tccagatcca tttccagaat cggttgccct
     2101 gcgcttacct gcgcaccctc ttccaccaga cgtttaaagc ctttaccttc cagcgctacg
     2161 gtgtcgatac ccatatggac gacgatctcc gcgccttttt cggtttccag gcagaacgcg
     2221 tggttggtgt tgaagatttt cacgattgtc cctgcggctg gtgatacgac gattttatct
     2281 gtcggtttca ccgccacacc gtcacccacc gctttgctgg cgaatgcttc gtcaggaacc
     2341 tgatccagtg ccacgacatc accggtaatc ggcgatacca gctccgcgat agataccgcg
     2401 tttggtacag cctgcggttt tgctacaggc gcggcagttg ccggagttgc ttcagctgac
     2461 gcagcggcta ccggaccacg ggcaacgact ttcttcatcg catcgccgat ggattctgct
     2521 ttcgcgccaa caatcacctg aatagtctgt ttgttcagtt tcactacacc agaagcaccc
     2581 agacgtttac acatcgtatc gttgacgcgg gcagagtcag ccacggtcag gcgcagacgg
     2641 gtaatacagg catcaatcgc tttcaggttg tcagtaccgc caaccgcagc aatatagttg
     2701 gttgccagtt gagtcagacc ttcttcagta ttgctgttgg cttcttcagt aacgatctcg
     2761 tcttctttat cttcacgacc cggcgttttc aggttgaaca tgcggataac caaactgaac
     2821 accacgaagt agatagcgaa gaagataacg cccatcacca gcagcatcca gacgttctgg
     2881 ctggcggccg gcaggttata catcaacgcg tagtcgatag cccccgcaga gaaagagaag
     2941 ccggcatgga tacccagcag cgttgccaca aacaggctga taccggtcag cagtgcgtgc
     3001 aggaggtaca gcagcggcgc aaggaacatg aacaggaatt ccagcggctc agtcacaccg
     3061 gtcaggaacg cagtaacagc aacagaaagc agcatcccgc caaccatcgg acgacgctct
     3121 ttcggtgctg cgaagtacat cgccagcgcc gcacccggca gaccgaacat catgatcggg
     3181 aagaagccgg acatgaacat ccccgcggtg ccgtcaccag cgtagaaacg gttgatgtcg
     3241 ccgtggaaaa ccgtacccac cgcgttggtg aattcaccaa tctggaacca ggcgatggtg
     3301 ttcagcacct gatgcagacc ggttgggatc agcagacggt tgatgaaacc aaagataccg
     3361 gaacccagcg cgcccgcaga aacgatccac tcgccgcctg catggatagc gtgctgtacc
     3421 ggcggccaga cgtaaccaaa aatggccgcc agcaccagac agaagaagcc ggtggcgatc
     3481 ggcacaaagc gtttgccgcc gaagaagctc aggaagtccg gcagtttaat atcggaccaa
     3541 cggttatagg ctgcgccacc aaccagaccg gtaatgatac ccgccagtac acccatgtta
     3601 atttctgggt tgatggtcac catcgctttg gttaacacaa agtaacctac cgcacccgcc
     3661 agcgccgccg cacctgcgct gtctttcgac cagctggatg ccacaccgat ggcgaagatt
     3721 aatgcgaggt tatcaaaaat tgcaccgccc gcctgggcaa taaacgcaac gttaagtaaa
     3781 tctggctgac cgaatcgcag caacagcgcc gccaccggca gcaccgcgat agggagctgt
     3841 aacgccctac cgagtcgctg gaaaaaacct aaaatattca tcttattccc cctacgagaa
     3901 ccctatttgg ctcgtttaaa gccgtatttt tattttgctg caaattttac tgccgatgtt
     3961 ctgtaatcag attgttagat catctgctac agagtgtgtg aaaatttaat tcgtatcgca
     4021 aattaaacgc gtgtcttttg tgagttttgt caccaaatat cgttatcatc actccctttt
     4081 actggctaaa ccagaaaact tattttatca ttcaaaaaat caagtcggat tgacgcctgt
     4141 ctgcgcaaat ccaggttacg cttaaagatg cctaatccgc caacggctta cattttactt
     4201 attgaggtga ataatgagac tgatccccct gactaccgct gaacaggtcg gcaaatgggc
     4261 tgctcgccat atcgtcaatc gtatcaatgc gttcaaacca actgccgatc gtccgtttgt
     4321 actgggcctg ccgactggcg gcacgccgat gaccacctat aaagcgttag tcgaaatgca
     4381 taaagcaggc caggtcagct ttaagcacgt tgtcaccttc aacatggacg aatatgtcgg
     4441 tctgccgaaa gagcatccgg aaagctacta cagctttatg caccgtaatt tcttcgatca
     4501 cgttgatatt ccagcagaaa acatcaacct tctcaacggc aatgccccgg atatcgatgc
     4561 cgagtgccgc cagtatgaag aaaaaatccg ttcttacgga aaaattcatc tgtttatggg
     4621 cggtgtaggt aacgacggtc atattgcatt taacgaaccg gcgtcttctc tggcttctcg
     4681 tactcgtatc aaaaccctga ctcatgacac tcgcgtcgcg aactctcgtt tctttgataa
     4741 cgatgtcaat caggtgccaa aatatgccct gactgtcggt gttggtacac tgctggatgc
     4801 cgaagaagtg atgattctgg tgctgggtag ccagaaagca ctggcgctgc aggccgccgt
     4861 tgaaggttgc gtgaaccata tgtggaccat cagctgtctg caactgcatc cgaaagcgat
     4921 catggtgtgc gatgaacctt ccaccatgga gctgaaagtt aagactttaa gatatttcaa
     4981 tgaattagaa gcagaaaata tcaaaggtct gtaattgtta tccctgccct ggttccttgc
     5041 tcagggcaat attttttaaa atcgggggtc agaatgtatg cattaaccca gggccggatc
     5101 tttaccggcc acgaatttct tgatgaccac gcggttgtta tcgctgatgg cctgattaaa
     5161 agcgtctgtc cggtagcgga actgccgcca gagatcgaac aacgttctct gaacggggcc
     5221 attctctccc ccggttttat cgatgtgcag ttaaacggct gcggcggcgt acagtttaac
     5281 gacaccgctg aagcggtcag cgtggaaacg ctggaaatca tgcagaaagc caatgagaaa
     5341 tcaggctgta ctaactatct gccgacgctt atcaccacca gcgatgagct gatgaaacag
     5401 ggcgtgcgcg ttatgcgcga gtacctggca aaacatccga atcaggcgtt aggtctgcat
     5461 ctggaaggtc cgtggctgaa tctggtaaaa aaaggcaccc ataatccgaa ttttgtgcgt
     5521 aagcctgatg ccgcgctggt cgatttcctg tgtgaaaacg ccgacgtcat taccaaagtg
     5581 accctggcac cggaaatggt tcctgcggaa gtcatcagca aactggcaaa tgccgggatt
     5641 gtggtttctg ccggtcactc caacgcgacg ttgaaagaag caaaagccgg tttccgcgcg
     5701 gggattacct ttgccaccca tctgtacaac gcgatgccgt atattaccgg tcgtgaaccg
     5761 ggcctggcgg gcgcgatcct cgacgaagct gacatttatt gcggtattat cgctgatggc
     5821 ctgcatgttg attacgccaa cattcgtaac gctaaacgcc ttaaaggcga caaactgtgt
     5881 ctggttaccg acgccaccgc gccagcaggt gccaacattg aacagttcat ttttgcgggt
     5941 aaaacaatat actaccgtaa cggactttgt gtggatgaga acggtacgtt aagcggttca
     6001 tccttaacca tgattgaagg cgtgcgtaat ctggtcgaac attgcggtat cgcactggat
     6061 gaagtgctgc gtatggcgac gctctatccg gcgcgtgcga ttgacgttga aaaacgtctc
     6121 ggcacgctcg ccgcaggtaa agtagccaac ctgaccgcat tcacacctga ttttaaaatc
     6181 accaagacca tcgttaacgg taacgaggtc gtaactcaat aagagaaagt atgacaccag
     6241 gcggacaagc tcagataggt aatgttgatc tcgtaaaaca gcttaacagc gcggcggttt
     6301 atcgcctgat tgaccagtac gggccaatct cgcggattca gattgccgag caaagccagc
     6361 ttgcccccgc cagcgtaacc aaaattacgc gtcagcttat cgaacgcggg ctgatcaaag
     6421 aagttgatca gcaggcctcc accgggggcc gccgcgctat ctccatcgtc accgaaaccc
     6481 gcaatttcca cgcaatcggc gtacggcttg gtcgtcatga cgccaccatc actctgtttg
     6541 atctcagcag caaagtgctg gcagaagaac attacccgct gccggaacgt acccagcaga
     6601 cgctggaaca tgccctgctg aatgccattg ctcagtttat tgatagctac cagcgcaagc
     6661 tgcgcgagct aatcgccatt tcggtgatcc tgccagggct tgttgacccg gacagcggca
     6721 aaattcatta catgccgcat attcaggtag aaaactgggg gctggtagaa gcactggaag
     6781 aacgttttaa agtgacctgt ttcgttggtc acgatatccg tagtctggcg ctggcggagc
     6841 actacttcgg tgcaagtcag gattgcgaag actccattct ggtgcgtgtc catcgcggaa
     6901 ccggggccgg gattatctct aacgggcgca tttttattgg ccgcaacggt aacgtcggtg
     6961 aaattggcca tattcaggtc gaaccgctgg gtgaacgctg ccactgcggc aactttggct
     7021 gcctggaaac tatcgccgcc aacgctgcca ttgaacaacg ggtgttgaat ctgttaaagc
     7081 agggctacca gagccgtgtg ccactggacg actgcaccat caaaactatc tgcaaagccg
     7141 cgaacaaagg cgatagcctg gcctcggaag tgattgagta tgtcggtcgt catctgggta
     7201 aaaccatcgc cattgctatc aacttattta atccgcaaaa aattgttatt gctggtgaaa
     7261 tcaccgaagc cgataaagtg ctgctccctg ctattgaaag ctgcattaat acccaagcgc
     7321 tgaaggcgtt tcgtactaat ctgccggtgg tacgttctga gctggatcac cgctcggcaa
     7381 tcggcgcttt tgcgctggta aaacgcgcca tgctcaacgg tattttgctc cagcatttgc
     7441 tggaaaatta atgtgctttt atagtggcgc ttattgttgt caatattctg ggtagtccat
     7501 gaccattaaa aatgtaattt gcgatatcga cggcgtgctg atgcacgata acgtcgccgt
     7561 accgggtgca gcggaatttt tgcacgggat tatggataaa ggcctgccgc tggtgttgct
     7621 gaccaactat ccttcgcaga ctgggcaaga tctggcgaac cgctttgcca ctgccggtgt
     7681 cgatgtgccg gatagcgtgt tttatacctc tgcgatggcg accgccgatt tcctgcgtcg
     7741 ccaggaaggc aagaaagcgt atgtggtggg cgaaggcgca ctgattcatg aactgtacaa
     7801 agccggtttc actattaccg atgtgaaccc tgatttcgtg attgttggcg aaacacgttc
     7861 ctacaactgg gacatgatgc ataaagcagc ctatttcgtc gctaacggtg cacgctttat
     7921 cgccaccaac ccggacaccc acgggcgcgg tttttatccc gcttgtggcg cgttgtgtgc
     7981 agggattgag aaaatctccg ggcgcaaacc gttctatgtt ggtaagccca gcccgtggat
     8041 catccgcgcg gcattaaaca aaatgcaggc gcattcggaa gaaacagtga ttgtcggcga
     8101 taacctgcgt accgatattc tggcgggctt ccaggccggt ctggagacga ttctggtgct
     8161 ttctggtgtt tcatcgctcg acgatatcga cagtatgccg ttccgcccaa gctggattta
     8221 cccgtcagtc gctgaaatcg acgttatctg aataccaccc acgtctgcgg acgtggttgg
     8281 ctctgatgac aaacgccaaa ctgcctgatg cgctacgctt atcaggccta cgcagctcct
     8341 gcaatatatt gaattttcat gcttttgtag gccggataag gcgttcacgc cgcatccggc
     8401 atgaacaaat cgcactttat cagcaatttg gcccccgtaa aatataacca ttcaataaaa
     8461 cccgctaaaa attatcgatt catcaataaa tacatcctca caccagaccc tttttcacca
     8521 atcgacaatc accattacgt tttttatttt tgcactgcca aatagcttgc gcacagtgct
     8581 gctttgcggc atttttttaa acaagcaaac acaacaagca acaaatacca ggttaacgga
     8641 gaaggttatg tgttcaattt ttggcgtatt cgatatcaaa acagacgcag ttgagctgcg
     8701 taagaaagca ctcgagctgt cacgcctgat gcgtcatcgt ggcccggact ggtccggtat
     8761 ttatgccagc gataacgcca ttctcgctca cgaacgtctg tcaattgttg acgttaacgc
     8821 gggggcgcaa cctctctaca accaacaaaa aacccacgta ctggcggtaa acggtgaaat
     8881 ctacaaccac caggcattgc gcgctgaata tggcgatcgt tatcagttcc agacagggtc
     8941 tgactgtgaa gtgatcctcg cgctgtatca ggaaaaaggg ccggaatttc tcgacgactt
     9001 gcagggcatg tttgcctttg ccctgtacga cagcgaaaaa gatgcctacc tgattggtcg
     9061 cgaccatctg gggatcatcc cactgtatat ggggtatgac gaacacggtc agctgtatgt
     9121 ggcctcagaa atgaaatcgc tggtgccagt ttgccgcacg attaaagagt tcccggcggg
     9181 gagctatttg tggagtcagg acggcgaaat ccgttcttat tatcatcgcg actggttcga
     9241 ctttgatgca gtgaaagata acgtgaccga caaaaacgag ctgcgtcagg cactggaaga
     9301 ttccgttaaa agccatctga tgtctgatgt gccttacggt gtgctacttt ctggtggtct
     9361 ggattcctca attatttccg ctatcaccaa gaaatacgca gcccgtcgcg tggaagatca
     9421 ggaacgctct gaagcctggt ggccgcagtt acactccttt gctgtaggtc tgccgggttc
     9481 accggatctt aaagcagccc aggaagtggc aaaccatctg ggcacggtgc atcacgaaat
     9541 tcacttcact gtacaggaag gtctggatgc catccgcgac gtgatttacc acatcgaaac
     9601 ttatgatgtg accactattc gcgcttcaac accgatgtat ttaatgtcgc gtaagatcaa
     9661 ggcgatgggc attaaaatgg tgctgtccgg tgaaggttct gatgaagtgt ttggcggtta
     9721 tctttacttc cataaagcgc ccaacgctaa agaactgcat gaagagacgg tgcgtaaact
     9781 gctggccctg catatgtatg actgcgcgcg cgccaacaaa gcgatgtcag cctggggcgt
     9841 ggaagcacgc gttccgttcc tcgacaaaaa attccttgat gtggcgatgc gcattaaccc
     9901 gcaggataaa atgtgcggta acggcaaaat ggaaaaacac atcctgcgtg aatgttttga
     9961 gtcatacctg cccgcaagcg tggcctggcg gcagaaagag cagttctccg atggcgtcgg
    10021 ttacagttgg atcgacaccc tgaaagaagt ggcggcgcag caggtttctg atcagcaact
    10081 ggaaactgcc cgcttccgct tcccgtacaa cacgccgacc tcaaaagaag cgtatctgta
    10141 ccgcgagatc tttgaagaac tgttcccact tccgagtgcc gctgagtgcg tgccgggcgg
    10201 tccttccgtc gcgtgttctt cagctaaagc gatcgaatgg gatgaagcgt tcaaaaaaat
    10261 ggacgatccg tctggtcgtg cggttggcgt tcaccagtcg gcgtataagt aagaaatgcg
    10321 gtactgcccc ggagaaatcc ggggctgttt tagttattgc gctgcttgtt gattctgata
    10381 tgcagtaagg aaatgttccg ggtttgccac atcactgttt gacaactcat cactgtaggc
    10441 cagtatttca tagattaatt tcccaccact attgttgaat tccgaatttc atcctgtacg
    10501 acaaaagaat ggctgtaggg tggggaaaaa acacaaagta aacaataatt gacgaatata
    10561 gcgccacgct gttcgcaacc taaccaaaca gtcactttcg agcaattttc cttgaaaaag
    10621 aggttgacgc tgcaaggctc tatacgcata atgcgccccg caacgccgat aaggtatcgc
    10681 gaaaaaaaga tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc
    10741 acaggttcga atcccgtcgt agccaccatc ttttttgcgg gagtggcgaa attggtagac
    10801 gcaccagatt taggttctgg cgccgcaagg tgtgcgagtt caagtctcgc ctcccgcacc
    10861 attcaccaga aagcgttgta cggatggggt atcgccaagc ggtaaggcac cggtttttga
    10921 taccggcatt ccctggttcg aatccaggta ccccagccat cgaagaaat
//
LOCUS       DBKEKI010000130        10832 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.131, whole genome shotgun sequence.
ACCESSION   DBKEKI010000130 DBKEKI010000000
VERSION     DBKEKI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.131"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..120
                     /locus_tag="ACUQPX_003344"
     CDS             <1..120
                     /locus_tag="ACUQPX_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057937.1"
                     /translation="LPINLTQETLFSSKSHGKYALFPIFTASWRATRIKNKGI"
     gene            343..840
                     /gene="ubiC"
                     /locus_tag="ACUQPX_003345"
     CDS             343..840
                     /gene="ubiC"
                     /locus_tag="ACUQPX_003345"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HHV9057938.1"
                     /translation="MSHPALTQLRALRYFKEIPALDTQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            853..1725
                     /gene="ubiA"
                     /locus_tag="ACUQPX_003346"
     CDS             853..1725
                     /gene="ubiA"
                     /locus_tag="ACUQPX_003346"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HHV9057939.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1880..4303)
                     /gene="plsB"
                     /locus_tag="ACUQPX_003347"
     CDS             complement(1880..4303)
                     /gene="plsB"
                     /locus_tag="ACUQPX_003347"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HHV9057940.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVSINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4474..4842
                     /gene="dgkA"
                     /locus_tag="ACUQPX_003348"
     CDS             4474..4842
                     /gene="dgkA"
                     /locus_tag="ACUQPX_003348"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HHV9057941.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            4952..5560
                     /gene="lexA"
                     /locus_tag="ACUQPX_003349"
     CDS             4952..5560
                     /gene="lexA"
                     /locus_tag="ACUQPX_003349"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HHV9057942.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5633..6958
                     /gene="dinF"
                     /locus_tag="ACUQPX_003350"
     CDS             5633..6958
                     /gene="dinF"
                     /locus_tag="ACUQPX_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HHV9057943.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7074..7283
                     /locus_tag="ACUQPX_003351"
     CDS             7074..7283
                     /locus_tag="ACUQPX_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HHV9057944.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7325..7840)
                     /gene="zur"
                     /locus_tag="ACUQPX_003352"
     CDS             complement(7325..7840)
                     /gene="zur"
                     /locus_tag="ACUQPX_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HHV9057945.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8158..9143
                     /locus_tag="ACUQPX_003353"
                     /pseudo
     CDS             8158..9143
                     /locus_tag="ACUQPX_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105478490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            9506..9610
                     /locus_tag="ACUQPX_003354"
                     /pseudo
     CDS             9506..9610
                     /locus_tag="ACUQPX_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            9864..10436
                     /locus_tag="ACUQPX_003355"
     CDS             9864..10436
                     /locus_tag="ACUQPX_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HHV9057946.1"
                     /translation="MFCEEKVAQMAAYLLLKRGGRMAYLKLMKLLYLSNRQSILKHGR
                     MIGEDSLYSMKFGPVMSNTLNLIRGKAEGIGDYWYNLIETNGHNVSLRSDPREMDADE
                     VFDELSRADIRILDEIYSRYGHMNRFDLANMTHLESVCPEWHDPGNSRKPIDLKEMLI
                     SEGKSEDEANRIIGKMEESQKLKEFSLQLS"
     gene            10466..10810
                     /locus_tag="ACUQPX_003356"
     CDS             10466..10810
                     /locus_tag="ACUQPX_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004953517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057947.1"
                     /translation="MLAPTGPCNHLHVICNDPVYYPVNDCYCVLVVNISSIKDGVPHD
                     PSCVLNSGDHRFIKHPSYVVYAEAIIWRVDNMVRKQRSGEISVHDDMPEATFNRILDG
                     FDISDEVMLPTY"
BASE COUNT         2677 a         2432 c         2806 g         2917 t
ORIGIN      
        1 ttgcctatta atcttactca ggaaacactt ttcagctcca aatctcatgg taaatatgcg
       61 cttttcccaa tttttactgc gagttggcga gctactcgta taaagaataa aggtatttaa
      121 gtaaaggaaa acatcaccgt tcctagcatc atgggcggtg atgcccctat ggttgccctc
      181 gccagcacgg gcatcggcaa agcgtaaggt tcaacagcgt tttaccactt catgcgattg
      241 ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt aaacactttt
      301 ctgcgataca atgcctttaa gttatgtaac ggagagttcg gcatgtcaca ccccgcgtta
      361 acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccttggatac gcaactgctc
      421 gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg aaaaacggtg
      481 agtgtgacga tgatccgcga ggggtttgtc gagcagaatg aaatccccga agaactgccg
      541 ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc cgatggtgaa
      601 ccgtggcttg ccggtcgtac cgtcgttcct gtatcaacat taagcgggcc ggaactagcg
      661 ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc gacactaacc
      721 cgggatttta tcgagatagg ccgtgatgcc ggactgtggg ggcgacgttc ccgcctgcga
      781 ttaagcggta aaccgctgtt gctaacagaa ctgtttttac cggcgtcacc gttgtactaa
      841 gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt tcatcgctta
      901 atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt atgggcgttg
      961 tgggtggcga caccgggcgt tcctcagctc tggatcctgg cggtgtttgt cgcgggtgtc
     1021 tggctgatgc gcgctgctgg ttgcgtggtg aatgattatg ctgatcgcaa gtttgatggt
     1081 catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga gaaagaggcg
     1141 cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggttctgac gctgaatacg
     1201 atgaccattc tgttgtcgat tgccgctctg gcattggcgt gggtgtaccc gtttatgaag
     1261 cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc gattccaatg
     1321 gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt cctcgccaat
     1381 attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga tgatgatgtg
     1441 aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt gattattggt
     1501 attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa tggcttaggc
     1561 tggggatatt actggtcaat tgtggtggct ggcgcgctgt ttgtttatca acaaaaactg
     1621 attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa ctatgttggt
     1681 ctggtactgt ttttaggact ggcaatgagt tactggcatt tctgatgatg taaaaaagcc
     1741 ggatgatcat ccggctttct tctgggttgc ctgatgcgct gcgcttctca ggcctacccg
     1801 gtgaatcgca atttattgaa tttgcaggtc atggtaggcc ggataaggcg ttttcgccgc
     1861 atccggcaat tctctctgat tacccttcgc cctgcgtcgc actctcaatc gtcaaacgca
     1921 cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct gctggttcgg
     1981 catcgccgct atcgctgata tacccttcat cacgcagcgt cagcaccaga gaactgaaca
     2041 ccgccttgtc gaagaactcc ggcgcgttga tgccgtgcag cacggagaga cgttgcgcaa
     2101 cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttggca ctcaacaacc
     2161 agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc agctgtaggg
     2221 tgcgagaatg cgccgggttg atatgcaact catcatcttg cagggtaatc agcccctgac
     2281 gttgcatctc atttgccagc gcatcaataa cgtccggcaa ctcgtcgcga tcccagcgca
     2341 ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc aatacgtcgc
     2401 gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc accaacatat
     2461 gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgtggc agaatgatga
     2521 tgtcgccgat agtgtctttc tcgacttcaa acttgttcat ttgcagcgcg tgatcgataa
     2581 gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg ttgcgcatca
     2641 gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtgagtgag cgctgacgtg
     2701 atgccagtag cgcagtacag cacaggttca tggcgtttgc cgcgcctgcg ttgttaatgc
     2761 ttaccatcag atcggcagca atattattga ccgtcggcgt taaccaggcc ggacgcaccg
     2821 cttcgatggg atcgatagat tcacgccagt ctggtacgtg ctggttaagg taggtcatca
     2881 acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc ttgcttaaac
     2941 cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc gccgcgcagt tctttggcgt
     3001 aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc gtaatcggac
     3061 gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt ttcggatcca
     3121 gcaaacgccc cgtgcgggaa cgaccgcctt ccacgaagta ctcgacggaa taaccacggc
     3181 tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg cctttaaaag
     3241 tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc cagaagttca
     3301 ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc acgtaagaaa
     3361 gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca tgaccgtcgt
     3421 gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa aatcggttcc
     3481 aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa ttcgccgcaa
     3541 tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt tttttgctgc
     3601 gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta ttaaacagat
     3661 cctgacgagc aggcagacgt gggcctacgg cagccagacg ttgacgggca aagtgcatac
     3721 gcgccacgcg cgccagtttc tgagcgatag ttttatccgt gccgtgttca tccgccatac
     3781 ggcgcagcga cactgacggc gagaaacgca caaaactgtc gcgaccgagc cacagtacag
     3841 cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gttcacttcg cctttttcac
     3901 gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc agatttgggt
     3961 tgctacggtg caaatcgaga tagtcgtgga aaagcttaat agactcttct ttcggcgtgt
     4021 aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt agcgtgccgt
     4081 cgatttccag cggctctaac gggtcaggca aatcatgtgc cagacactgg gcgcgcaacg
     4141 tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga gaggtatcca
     4201 gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg atgcttaatg
     4261 gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat gtaaagcctc
     4321 tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat tctgtggtat
     4381 ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac ggtacactga
     4441 tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac tggattcacc
     4501 cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg gatcaacgaa
     4561 gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc ctgctggctg
     4621 gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt gatgattgtg
     4681 gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga ataccatgag
     4741 ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgccat tatcgtcgcc
     4801 gtgattacct ggtgcatcct gttatggtcg cattttggat aacccttcca gatttcgata
     4861 aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt atatactcac
     4921 agcataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca ggcaacaaga
     4981 ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga cgcgtgcgga
     5041 aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc tgaaggcgct
     5101 ggcacgcaaa ggcgttattg aaattgtttc cggcgcatca cgcgggattc gtctgttgca
     5161 ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac cgcttctggc
     5221 gcaacagcat attgaaggtc attatcaggt cgatccttcc ttgttcaagc cgaatgctga
     5281 tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatc ggcattatgg atggcgactt
     5341 gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg cacgtattga
     5401 tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac tgttgccaga
     5461 aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca ccattgaagg
     5521 gctggcggtt ggggttattc gcaacggcga ctggctgtaa catatctctg agaccgcgat
     5581 gccgcctggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct gcatggcatt
     5641 cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt tctccaatat
     5701 caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg atagtccggt
     5761 ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta tgctgttgct
     5821 gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca aaaatcctca
     5881 ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggccg gggcgttaat
     5941 tgcgctgttg cgtacgccga ttatcgatct ggcgctgcat attgttggcg gtagtgaagc
     6001 ggtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgctc cggcgtcgct
     6061 ggcgaatctg gtattactcg gttggttact cggcgtgcaa tatgcccgtg cgccagtaat
     6121 tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg tgatggggct
     6181 gcatatgaac gtgcagggcg cggcgctggc gacggttatt gcggaatatg caacattgct
     6241 gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg gcgaaatgct
     6301 gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg atatcatgct
     6361 gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg cgcgactggg
     6421 gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta ccgcctatgc
     6481 gcttgatggt tttgcctacg cggttgaagc gcactccggt caggcatacg gtgcgcgcga
     6541 cggtagccag ttgctggatg tatggcgggc agcgtgccgc cagtcgggga ttgtagcgtt
     6601 actgttttcg gtggtttatt tgctggcagg ggaacacatc attgcgttgc tgacgtcgtt
     6661 aacccagatt cagcagctgg ctgaccgcta tcttatctgg caggtgattt tgccgttggt
     6721 tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgtg ccgccgaaat
     6781 gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta cgctgccgtg
     6841 gctgggtaat catggtttgt ggctggcatt aaccgtcttt ctggcgttgc gcgggctttc
     6901 tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg caacgtgacg
     6961 gttaaaaatt ctgaataaat tatcctaagc caaattgctg actacactta atctcacgtt
     7021 cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg attatgaata
     7081 aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag caatggggca
     7141 aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg gtcggtaaaa
     7201 tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg gattgggaaa
     7261 cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa ggatgtacgc
     7321 tctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac tgttcaggat
     7381 gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca atcacattat
     7441 gccgcagggc aaaccccatt tttgccgcca gcgtatgcat aatgtcttcc acgccttctg
     7501 cacactcttc tttcaccgcg ccgcagcgat cgcaaataaa catggctgac gtatgcgtgg
     7561 gctggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta tgcacaaaac
     7621 cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct tgcggttcag
     7681 cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc agactcatca
     7741 ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg cagatttttt
     7801 cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc aaagtggggg
     7861 ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct tattgttgcc
     7921 gtaatgttga ttttctgttt tgtaggtaag gtgttgtgtt gccttgtcgt accattatca
     7981 acacgataat aattaataat tcatttttaa ataaattgct tcaagttaac tcgctattta
     8041 tttaactggg tttaaacgca tacctgtagt tcagaaatta tgagcgattt ttgatagtga
     8101 ccagatggca aaagaattat aaggaggaca cggtacgaca aaaaaggtag atgaatcatg
     8161 ttgaagataa tacccggtgc aactggatac tttaataaaa cactcaattc aaatcaattc
     8221 gaaaataaag atgcaaccaa agataaattg gataatagag gttcaatcaa aggtaaatta
     8281 aataatatat atggtaaatc tattgattat gcagcgctgc gtcatcggga tataattatt
     8341 gcaaaaatag atttgtttat tcaacgaatc acgcataatt tatggcatgc acgtaaaaaa
     8401 atgtgttttt aattgagcaa ataaatgatc tcaaaatgtg ggtcaataag tatactgacg
     8461 attgcactga tgaggattta aacgatcgtg actttattgc atcagttgtt gaccgggcta
     8521 tttttcattt cgcgattaat agtatatgta atcctgggga taataaagat gcaatgccca
     8581 ttgaacaatg tacttttgat gtagaaacta agaatggcct tccctccacg gttcagctat
     8641 tttatgagga atctaaggat aatgaacctt tagcgaatat acattttcaa gcaataggtt
     8701 ctggtttttt aacgtttgtt aatgcctgcc aggaacatga tgacaacagc ttaaaattat
     8761 ttgcttcgct gttaatttca ctttcatatt ctagtgccta cgcagattta tcagaaacag
     8821 tgtatattaa tgaaaataat gagagctacc tgaaagctca gtttgaaaaa ttatatcaac
     8881 gtgatatgaa gaagtacctg ggagagatga agcgtctggc agatggggga gaaatgaatt
     8941 ttgatggcta tctggataag atgtcacatc tggtgaatga aggaacgctc gatcctgata
     9001 ttttaagcaa aatgcgagat gctgcaccac aattaattag cttcgcgaag tcgtttgacc
     9061 caacctcaaa ggaagagatt aaaatactta cagacacttc taaattaatt tatgacttgt
     9121 tcggggttaa atcggagaaa taatatgtga agttcttcga tagtatggaa ggccttatat
     9181 aaaaggaccc aatatttatt gggttctttt ttctctatca atgctattag cagggagata
     9241 tatcaccaga gtttaatgtg tgatttttta tttatcgtcg aacatggatt gtttatcatt
     9301 ggccttaaca aagttaacgg ctaataagat tatttccatc acttcgtgag agcttcatgc
     9361 cttgagagga tctcaatttt cttttgcaat gagacaggcg cttcctgttg ttatggtata
     9421 gtaccccgct attgagcctc ctgaacagtg aagctgaata acataacccc atgatatatc
     9481 gataaaataa tctctacatt tgaaaatgca cggtaattct gaaatgcaaa aaatcaacca
     9541 aaccagagca atgcctgaaa aaactgacgt tcactggagt ggtcggttta gcgttgcacc
     9601 aatgctcgat aggatgtacc gtttttgaaa aacaactagt tatacacttt gtgggagcct
     9661 attgggaacc tgttattctt agaaagcatc atttgctcat cacttcaatt gaccatggtt
     9721 aaagccagct ataaacttga caaacgaggt aactggttgc ttaggcccaa gtgggcacac
     9781 cataagaagc tcatgttggt gatgaagcaa cccatgaagt atcgagaata attataaagt
     9841 tcactatctt tgaggtttct tatatgtttt gcgaagaaaa agtagctcaa atggctgcat
     9901 acctacttct taagagaggt gggcgcatgg catatctgaa attgatgaag ctgctctatc
     9961 tgtctaaccg ccagtcgatt ttgaagcatg gcaggatgat cggcgaagat agcctttact
    10021 ctatgaaatt tggaccagtc atgtcgaata cgctgaactt gattcgcggt aaggctgaag
    10081 gcattggtga ctactggtac aacttgatag agacgaacgg gcataatgta tcgttgcgtt
    10141 cagatccgag ggaaatggat gcagacgagg tctttgatga attgagtcgt gcagatatcc
    10201 ggattttaga tgaaatctat tctcggtatg ggcatatgaa ccgatttgat ctcgcaaata
    10261 tgacgcattt agaaagcgtt tgtccagagt ggcacgatcc tggcaattct cgtaagccta
    10321 tagacctgaa agaaatgctg atcagtgagg gtaaaagcga ggatgaggct aatcgcataa
    10381 ttggcaaaat ggaagaatct cagaaactta aggaattttc tttgcaatta tcatgacgga
    10441 ttatcagcca tacaggaaag gaactgtgct tgccccaact ggaccatgca atcatcttca
    10501 tgtgatttgt aatgatcctg tttattaccc cgttaacgat tgttattgtg ttttagttgt
    10561 taatatttct agtatcaagg atggtgtccc ccacgatccg tcttgcgtct tgaattctgg
    10621 tgatcatcgc tttatcaagc atccaagtta tgttgtttac gctgaagcta taatttggcg
    10681 agtggataac atggttagaa agcagcgatc gggtgagatt tctgttcatg atgatatgcc
    10741 agaagctaca ttcaatagaa ttctggacgg ttttgatatc tctgatgagg taatgctgcc
    10801 aacttactga tttagtgtat gatggtgatt tt
//
LOCUS       DBKEKI010000131        10809 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.132, whole genome shotgun sequence.
ACCESSION   DBKEKI010000131 DBKEKI010000000
VERSION     DBKEKI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.132"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            91..308
                     /locus_tag="ACUQPX_003357"
                     /pseudo
     CDS             91..308
                     /locus_tag="ACUQPX_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304813.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            524..1642
                     /locus_tag="ACUQPX_003358"
     CDS             524..1642
                     /locus_tag="ACUQPX_003358"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HHV9057948.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWNIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIVLVKKHCETWAGD"
     gene            1708..1956
                     /locus_tag="ACUQPX_003359"
     CDS             1708..1956
                     /locus_tag="ACUQPX_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HHV9057949.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2021..2389
                     /locus_tag="ACUQPX_003360"
     CDS             2021..2389
                     /locus_tag="ACUQPX_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HHV9057950.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDQQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2483..3136
                     /gene="nfsB"
                     /locus_tag="ACUQPX_003361"
     CDS             2483..3136
                     /gene="nfsB"
                     /locus_tag="ACUQPX_003361"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HHV9057951.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3244..4491
                     /gene="ybdG"
                     /locus_tag="ACUQPX_003362"
     CDS             3244..4491
                     /gene="ybdG"
                     /locus_tag="ACUQPX_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HHV9057952.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4559..5935)
                     /gene="pheP"
                     /locus_tag="ACUQPX_003363"
     CDS             complement(4559..5935)
                     /gene="pheP"
                     /locus_tag="ACUQPX_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HHV9057953.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASVLNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6036..9179)
                     /gene="cusA"
                     /locus_tag="ACUQPX_003364"
     CDS             complement(6036..9179)
                     /gene="cusA"
                     /locus_tag="ACUQPX_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HHV9057954.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9191..10414)
                     /gene="cusB"
                     /locus_tag="ACUQPX_003365"
     CDS             complement(9191..10414)
                     /gene="cusB"
                     /locus_tag="ACUQPX_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HHV9057955.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFEQAEPHAEKMPTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRHLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPNKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSGGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10430..10762)
                     /gene="cusF"
                     /locus_tag="ACUQPX_003366"
     CDS             complement(10430..10762)
                     /gene="cusF"
                     /locus_tag="ACUQPX_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HHV9057956.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
BASE COUNT         2620 a         2977 c         2630 g         2582 t
ORIGIN      
        1 cgtgccgaga tgatcctgta accatcatca gtagtgaagt agtgattcac gacttcaagg
       61 cgcttttcaa aagggtattt tggctttgac atattagggg ctattccatt tcatcgtcca
      121 acaaaatggg tgcagtacag ggttgataga aatatctcct ggggcttcat ctttccagac
      181 ctcagagtaa acctgaaatc ggaaagcttc aggcacccgc agttatctta cccttcctca
      241 acaaaatagc tgtttattta tacagcaaat cctgtttcgg acttctcact ccccatttga
      301 tcaactgaag ctgaacccgt ttttttctgc atgcctcaat cgtcaaaatt cttcgccacc
      361 agccagctct gccataactt gccacattga aaaagtagcc aaagtgcttc aggcaccatc
      421 agttcaccat caccaaaaaa aagaaacatt ccagaacttc cgcatctcgc ccagccgctc
      481 cgacttctat actgaataga aaacgtcaac ttaagagaaa cctatgccat tacccgattt
      541 tcatgtttct gaacctttta ccctcggtat tgaactggaa atgcaggtgg ttaatccgcc
      601 gggctatgac ttaagccagg actcttcaat gctgattgac gcggttaaaa ataagatcac
      661 ggccggagag gtaaagcacg atatcaccga aagtatgctg gagctggcga cggatgtttg
      721 ccgtgatatc aaccaggctg ccgggcagtt ttcagcgatg cagaaagtcg tattgcaggc
      781 agccgcagac catcatctgg aaatttgcgg cggtggcacg cacccgtttc agaaatggca
      841 gcgtcaggag gtatgcgaca acgaacgcta tcaacgaacg ctggaaaact ttggctatct
      901 catccagcag gcgaccgttt ttggtcagca tgtccatgtt ggctgtgcca gtggcgatga
      961 cgccatttat ttgctgcacg gcttgtcacg gtttgtgccg cactttatcg ccctttccgc
     1021 cgcgtcgcca tatatgcagg gaacggatac gcgttttgcc tcctcacgac cgaatatttt
     1081 ttccgccttt cctgataatg gcccgatgcc gtgggtcagt aactggcaac aatttgaagc
     1141 cctgtttcgc tgtctgagtt acaccacgat gatcgacagc attaaagatc tgcactggaa
     1201 tattcgcccc agtcctcatt ttggcacggt ggaggttcgg gtgatggata ccccgttaac
     1261 ccttagccac gcggtaaata tggcgggatt aattcaggcc accgcccact ggttactgac
     1321 ggaacgcccg ttcaaacatc aggagaaaga ttacctgctg tataaattca accgtttcca
     1381 ggcctgtcgc tatggtctgg aaggcgtcat taccgatccg cacactggcg atcgtcgacc
     1441 gctaacggaa gataccttgc gattgctgga aaaaatcgcc ccttcagcac ataaaattgg
     1501 cgcatcgagc gcaattgaag cactgcatcg ccaggtcgtc agcggtctga atgaagcgca
     1561 gctgatgcgc gatttcgtcg ccgatggcgg ctcgctgatt gtgctggtga aaaagcattg
     1621 tgagacctgg gccggtgact aagccgggaa ttgccttaca gaagaatatc cctgacaatg
     1681 gtgttttaac ttacatttaa caatgttatg aaacaccctt tagaaacctt gaccaccgca
     1741 gcaggcattt tgctgatggc tttcctctct tgcctgctgc tgcccgcccc cgcactgggg
     1801 ctgacgctgg cacaaaaact ggtgaccatg ttccatctga tggatcttag ccagctttac
     1861 actttattgt tttgtctgtg gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc
     1921 tttatctggc gacgctggtt ctcgctggcg gattaaacgt cgattatcgc cgaacttcgc
     1981 ggcatacttt gcttattctc cttcgcctaa aggaacgttt atggataagc aatcactgca
     2041 cgaaacggcg aaacgcctag ctcttgagtt accctttgtc gagctttgct ggccttttgg
     2101 cccggagttc gatgttttta aaattggcgg caagattttt atgctgtcgt cggagctacg
     2161 cggcgtcccc tttatcaatc tgaagtccga tcaacaaaaa tccctgttaa atcagcaaat
     2221 atacccaagc attaagccag ggtatcacat gaataaaaag cactggattt cggtgtattc
     2281 cggcgaggaa atctccgaag cgttacttcg cgatctgatc aacgattcgt ggaatctggt
     2341 ggttgatggt ctggctaaac gcgatcaaaa aagagtgcgt ccaggctaaa gcgggaatct
     2401 atagcgcatt tttctcgctt accatttctc gttgaacctt gtaatctgct ggcacgcaaa
     2461 attacttaca catggagtct ttatggatat catttctgtc gccttaaagc gtcattccac
     2521 taaggcattt gatgccagca aaaaacttac cccggaacag gccgagcaga tcaaaacgct
     2581 actgcaatac agcccatcca gcaccaactc ccagccgtgg cattttattg ttgccagcac
     2641 ggaagaaggt aaagcgcgtg ttgccaaatc cgctgccggt aattatgtgt tcaacaaacg
     2701 taaaatactt gatgcctcgc acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc
     2761 ctggctgaag ctggttgttg accaggaaga tgccgatggc cgctttgcca cgccggaagc
     2821 gaaagccgcg aacgataaag gtcgcaagtt cttcgccgat atgcaccgta aagatctgca
     2881 tgatgatgca gagtggatgg caaaacaggt ttatctcaac gtcggtaact tcctgctcgg
     2941 cgtggcggcg ctgggtctgg acgcggtgcc catcgaaggt tttgacgccg caatcctcga
     3001 tgcagaattt ggtctgaaag agaaaggcta caccagtctg gtggttgttc cggtaggtca
     3061 tcacagcgtt gaagatttta acgctacgct gccgaaatct cgtctgccgc aaaacaccac
     3121 cttaaccgaa gtgtaattct ctcttgccgg gcatctgccc ggctatttcc cctcagatta
     3181 tcctgatttg cataaccctg tttcagccgt catcataggc tgctgttgta taaaggagac
     3241 gttatgcagg atttaatatc ccaggttgaa gatttagcgg gtattgagat cgatcacacc
     3301 acctcgatgg tgatgatttt cggtattatt tttctgaccg ccgtcgtggt gcatattatt
     3361 ttgcattggg tggtactgcg gaccttcgaa aaacgtgcca tcgccagttc acggctttgg
     3421 ttgcaaatca ttacccagaa taaactcttc caccgtttag cttttaccct gcaggggatt
     3481 atcgtcaata ttcaggcggt attctggctg caaaaaggca ccgaagcggc agatattctg
     3541 actacctgcg cgcagttgtg gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg
     3601 gatgttattt tgaatctggc gcagaaattc ccggcggcat ctcagttacc gctgaaaggg
     3661 atatttcagg ggattaaact gatcggcgca atcctggtcg gcattttgat gatctcgctg
     3721 ctgattggtc agtcgccagc gattctgatc agcggtcttg gtgcaatggc tgccgtgctg
     3781 atgttggtat ttaaagatcc gattcttggt ctggtggcag gtattcagct ttccgcgaac
     3841 gatatgctga aactgggcga ctggctggaa atgccgaaat acggcgcgga tggcgcggtg
     3901 atcgatattg ggttaaccac cgtcaaagtg cgtaactggg acaataccat taccactatt
     3961 cccacctggt ctctggtttc tgactccttt aaaaactgga gcgggatgtc agcatctggc
     4021 gggcgacgta ttaagcgcag tatcagtatt gatgtcacca gtattcgttt tcttgatgaa
     4081 gacgaaatgc aacgtctgaa taaagcgcat ttgttaaagc cttatttaac cagccgccat
     4141 caggaaatta atgagtggaa tcgccagcaa ggttctacgg agtcggtatt aaacctgcgc
     4201 cgaatgacca atattggaac ctttcgtgcc tatctgaacg aatatctgcg taaccatccg
     4261 cggattcgta aagatatgac cttaatggta cgccaactgg ctccaggtga taacggttta
     4321 ccgctcgaga tctacgcgtt tactaacacc gtggtgtggc tggaatatga aagcattcag
     4381 gctgatatat tcgatcacat atttgcgatt gtcgaagagt ttggtctgcg acttcatcag
     4441 tcgccaaccg gcaatgatat tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa
     4501 ccgctctcat cgaatggatg agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt
     4561 atttccgacg cagcgtttta aatgccacaa acaggaatac aatccacacc ggcagcagga
     4621 tcgctgacaa gcgcatatca tccatcgtgc acatcagcag caaaatcatg ccgaggaagg
     4681 cgatgcaaag atagttgccg aacggataga gcagcgcctt aaactgtgtt tcacgcccct
     4741 gacgtcgcat cgccgcgcga aaacgcagat gcgccaggca gatcataatc cagttcaaca
     4801 gcaacgttgc taccaccagc gccatcagca gaccaaacgc tttttgcggc agcagatagt
     4861 tgattaacac caccagcgaa gtgatcgctc cggaaagcat cagcgagtta atcggcacac
     4921 cgcgacggct gacgcgagtc aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca
     4981 gcatgcggct gttagagtaa accccgctgt tatacaccga cagcgatgcc accagaatga
     5041 cgaagttcag cacagaagct accacattgc tgtcgagatt atggaaaatc atcacaaacg
     5101 ggctactgtt ggatttcact tccacccacg gatagagtgc cagtaaaacc accagtgaac
     5161 cgatataaaa cagcaggatg cgatacacca cctgatttac cgcttttgga atgctttttt
     5221 ccggatcgcg cgcttcagcg gctgtaatcc caatcagctc cagaccgccg aaggagaaca
     5281 taattaccgc cagcgacaaa atcagcccat tccagccggt ggcgaagaaa ccaccgtagc
     5341 gccagaggtt gtcgatactg gctttctcgc cgccgtgacc agaaaacagc agccacaggc
     5401 caaagccgat cataccgatg atcgccagca ctttaatcag cgcaaaccag aactcggttt
     5461 cgccatataa gcgcacgttc accaggttaa cggcgttgat gataataaag aaggcggcag
     5521 cccaaatcca cgttggaaca tccgggaacc agtactgcat atagatgccc gcagcggtca
     5581 gctctgccat tcccaccagc acgaacatta cccagtagtt ccagccagag aggaagcccg
     5641 caaacggtcc ccagtattta taggcaaagt gggcaaatga accggatacc ggctcttcga
     5701 ccaccatctc gccaagctgg cgcataatca ggaaagcgat gatcccggcg acgccgtagc
     5761 ccagcaatac agccggaccc gccatctgaa tcgccgggcc aatgccaaga aacagaccag
     5821 taccaattgc gccacccagc gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa
     5881 gcgtcggctc ttgattcgac gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt
     5941 tcccctgtgt gtcttttttg ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa
     6001 gacacaatcc acacggttaa acgaggtatc ctgctttaat tccgtacccg atgtcggtgc
     6061 agccacatca gcttatacgc cgccgggata ataaacagcg acagcaaagg tgcggtgatc
     6121 atgccgccaa tcatcggtgc ggcaatccgg ctcatcacct ctgaaccagc ccccgttccc
     6181 cacagaatcg gcagcagacc ggcgataatc accgccaccg tcatcgcttt cgggcgcacg
     6241 cgcaggaccg cgccgtgata caacgcctca tccagcttct gctcgctgaa tgtttgtgga
     6301 ttattcaacg acggctcggc ctctatggcg tgacgtaaat acatcagcat caccacgcca
     6361 aattcggcgg cgaccccggc aagggcaata aagccggtac ccgtcgccac agaaagatga
     6421 aagcccatcc accacagtaa ccagatgccg cccaccagcg caaacggtac gctgctgata
     6481 atcagcaacg cttcgcccac ccgacggaat gccagataca acagcacgaa gatgatcatc
     6541 agtgtcatcg gcaccatgag tttcagctta tggttggcgc gctccagtag ctcgaactgc
     6601 ccggagaatg ccacgctggt gcccggtttt aactgcactt tttcagctat cgctttttgc
     6661 aaatcgtgaa ccaccgacac catgtcacga tcgcgggcat cgatataaat ccagctcgtc
     6721 gggcgcgcat tctcggtttt cagcatcgac ggtccggtag agactttaac gtcggccacg
     6781 tctgccaggg tgatttgctg cttcatcggc gtcaggatcg gcaactggcg cagtgcctgc
     6841 ggactatcgc gccagctttg cggataacgc agattaattg gataacgggc aatcccttcc
     6901 accgtttcgc caaccatcgc cccgcccacc gcagaagtca caaacaactg cacatccgcc
     6961 accgtcatac cgtaacgtgc ggctttttca cggttaatct caacgttgat atagcgccca
     7021 ccttccagcc gctcggcaag cgcagaagct acgcctggca ccgttcgcgc cacttcttca
     7081 atttgctcag ccatcgcgtc gatatccgcc agcacagtgc cggaaacttt aatgccgatg
     7141 gggcttttaa tgccggttga gagcatatcg atacggttac gaattggcgg cacccacaga
     7201 ttcgccagcc ccggcagacg cacggtgtta tccagttcct caatgatttt gtccattgtc
     7261 atgcctggcc gccactgatc ctgtggctta agctggatgg tcgtttctac catctccagc
     7321 ggagcagaat cggtggcggt ttccgctttc ccggttttgc caaatacccg cgccacttca
     7381 ggtacgctca taattagctt gtcggttttt tgcagcatac tcgccgcctc tgctgcggaa
     7441 atccccggca gcgtcgatgg catatacaac aagtcgcctt cattgatctg cggtaaaaat
     7501 tccccgccaa ctttattgag cggccagaga accgtcagca ccgaaagcgc cgccaccagc
     7561 agcgtggttt tcggccagtg cagtactttc agcaacagcg gatgataaac acgaatcaaa
     7621 aagcgattga gcggattact gctttccggc ggaattttgc cacggatcca gtaacccatc
     7681 aggataggga tcactacgat cgccagcagc gccgcacccg ccatcgcata cgttttggtg
     7741 aacgccaacg ggccaaacag acgcccttcc tgcccttcca gggtgaagat cgggataaac
     7801 gacaacgtga taatcagcag actgataaac agcgccggcc ccacttcaac agacgcatcg
     7861 gtgatcacct gccagcgcgt tttattatcc agcgtggcgt caggatgctg gtgctgccac
     7921 tcttccagcc gtttatgcgc attctcgatc atgacgatag cggcatcgac catcgccccg
     7981 acggcaatcg caatgccacc cagcgacata atattggcat tcagcccctg gaagggcatg
     8041 acaataaaag caatacacaa ccccagcggc aacgaaataa tcgccaccag cgccgagcgc
     8101 acatgccaga gaaacagcgc gcagactacc gccaccacaa taaactcttc cagcaacttg
     8161 ccgctgaggt tgtcgatggc ccggtcaatg agctggctgc ggtcgtatgt tgtaactatc
     8221 tccacgcctt ccggcagact acttttcagc gtttccagtt tgtccttcac ggcggcgatc
     8281 acttctcggg cgtttttgcc ggatcgcagg atcaccaccc cgcccgccac ttcgccttcg
     8341 ccgtttagtt cggcaatgcc ccggcgcatc tccgggccaa tctggacctt cgcaacatcg
     8401 cgcagataaa cgggcacgcc attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg
     8461 agcgtttgta gatagccgct ggcgcgcacc atatattctg cttccgccag ttcgattgac
     8521 gaaccgcccg cttcctggtt tgaagcatcc agcgcgcttt ttacttcagc gagactgatg
     8581 ccatactgcg ccaggcgctg agggtcgata accacctgat actctttcac cacaccgcct
     8641 accgacgcca cttccgcaac gtcaggaatg gttttcagct catatttgag aaaccagtcc
     8701 tgcaatgagc gtaaatcggc aagatcgtgc ttaccgctgc gatccaccag tgcatattca
     8761 tagatccagc caacacccgt ggcatctggt cccagctcgg cgctgactcc cgcaggcagc
     8821 ttaccctgta cctggttgag gtactccagc acccgcgaac gcgcccagta cggatcggtg
     8881 ccatcttcga aaatgacata cacataagag tcgccaaact gcgagaaacc acgcacagtc
     8941 ttcgcgccag gcaccgacaa catggtggtg gttagcggat aagtcacctg attttcaacg
     9001 atttgcggtg cctggccggg atagctggtt ttaataatca cctgcacatc ggagagatcc
     9061 ggcaacgcat ccactggcgt attaatgatg gtccaggtgc cccagatgct cagaaacaac
     9121 gcgcccatca gcaccaaaaa acggttcgcc accgagcgac gaataatcca ttcaatcatt
     9181 ggttattccc tcaatgcgca tgggtagcac tttcagagcg catccgctcc agtgcgccag
     9241 aaatattggc ttcggaatca atcaggaaca ggccgccgga aaccaccttt tcaccttccg
     9301 ccagaccaga gcgtaatgcg gtgacgcctt gcgatgcctg gaaaacagca acgcgtttcg
     9361 gtacaaagcg cccgtcggca tcaacggtaa tcacccgctg ttcgttgccg gtatcaatca
     9421 gcgcctgtga cggaatgagc agcatcggtt cgctggcggt gttgagttgc aaccaggcgt
     9481 tcatgcccgg ttttagcgcc tcgtcggcgt tgtcgacttc cagacgcagc tgcagcgtgc
     9541 gggtcgcggc atccacgcca ggtagcagcg tccatttgcg gatggtgagt gttttattcg
     9601 gtcgcgccgg aacggtgagg gtaaactgcg aggcatcttt caccagccag gcgatggact
     9661 ccgggatcgc agcagtgacc cacaccgggt ccataccctg aattttcgcg accacgttat
     9721 ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg
     9781 ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga atatccgcct
     9841 ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc gccgtaccgc
     9901 cggtttcgcg cagcagtaaa tactcactct gcgcttccac ccagtcggga atggtcaggt
     9961 cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac accttgtcga
    10021 taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa ctgacattcg
    10081 ccgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt ttcaccccca
    10141 gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct tcatcggcat
    10201 atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg aaccgcgtat
    10261 tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc attttttctg
    10321 cgtgcggttc cgcctgttca aaccaggtaa aacctgccgc tgaaataata ccgcccgcga
    10381 tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt tactggctga
    10441 ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac gccactttgt
    10501 cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc atggtcatct
    10561 ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg ctttccagat
    10621 caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg ctcatggttt
    10681 catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga ctgaacattg
    10741 cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt gctgccaacc
    10801 gccccctaa
//
LOCUS       DBKEKI010000132        10806 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.133, whole genome shotgun sequence.
ACCESSION   DBKEKI010000132 DBKEKI010000000
VERSION     DBKEKI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.133"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACUQPX_003367"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACUQPX_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HHV9057957.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="ACUQPX_003368"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="ACUQPX_003368"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HHV9057958.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIIARMAA
                     TIDSTSGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="ACUQPX_003369"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="ACUQPX_003369"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HHV9057959.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="ACUQPX_003370"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="ACUQPX_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HHV9057960.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4020
                     /gene="rutD"
                     /locus_tag="ACUQPX_003371"
                     /pseudo
     CDS             3215..4020
                     /gene="rutD"
                     /locus_tag="ACUQPX_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
     gene            4030..4620
                     /locus_tag="ACUQPX_003372"
     CDS             4030..4620
                     /locus_tag="ACUQPX_003372"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HHV9057961.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4631..5125
                     /gene="rutF"
                     /locus_tag="ACUQPX_003373"
     CDS             4631..5125
                     /gene="rutF"
                     /locus_tag="ACUQPX_003373"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HHV9057962.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     SVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5146..6474
                     /gene="rutG"
                     /locus_tag="ACUQPX_003374"
     CDS             5146..6474
                     /gene="rutG"
                     /locus_tag="ACUQPX_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HHV9057963.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6556..6729)
                     /locus_tag="ACUQPX_003375"
     CDS             complement(6556..6729)
                     /locus_tag="ACUQPX_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HHV9057964.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            7102..7698
                     /gene="wrbA"
                     /locus_tag="ACUQPX_003376"
     CDS             7102..7698
                     /gene="wrbA"
                     /locus_tag="ACUQPX_003376"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HHV9057965.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7719..7946
                     /locus_tag="ACUQPX_003377"
     CDS             7719..7946
                     /locus_tag="ACUQPX_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HHV9057966.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7984..9225)
                     /gene="agp"
                     /locus_tag="ACUQPX_003378"
     CDS             complement(7984..9225)
                     /gene="agp"
                     /locus_tag="ACUQPX_003378"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HHV9057967.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9518..10774)
                     /locus_tag="ACUQPX_003379"
     CDS             complement(9518..10774)
                     /locus_tag="ACUQPX_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HHV9057968.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYVYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
BASE COUNT         2554 a         2762 c         2711 g         2779 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccggcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgtgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcatcgccc
     1321 gtatggccgc aaccatcgac tccacctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtgtccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccgcag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcattttg cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag tatttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttaacg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctgcat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttgcg ttaacggctg gctacgaata aacgcccata cgcgccgctg ttttcaggtt
     3601 cgcgaacgat tactgtatag cggcggcgcg caagcatggg tggaagcgca gccgttgttc
     3661 ctctatcccg ccgactggat ggcggcccgc gcacctcgcc tggaggcaga agacgcgctg
     3721 gcactggcgc attttcaggg caaaaataat ttactacgtc gacttaacgc cctcaaacgc
     3781 gctgacttta gtcaccatgc ggatcgcatc cgctgcccgg tgcaaatcat ctgcgccagt
     3841 gatgatctgc tggtgccaac agcatgttcc agtgaacttc atgccgccct gcccgatagc
     3901 cagaaaatgg tgatgcccta tggcggacac gcctgcaacg tgaccgatcc cgaaacgttt
     3961 aatgctctgt tactcaacgg gcttgccagc ctgttacatc accgtgaagc cgccctgtaa
     4021 ggaattgcta tgaacgaagc cgttagccca ggtgcgctta gcaccctgtt caccgatgcc
     4081 cgcactcaca acggctggcg ggagacaccc gtcagcgatg agacgttaca ggagctttat
     4141 gccctgatga aatgggggcc gacatcagct aactgttctc cggcacggat cgtgtttatc
     4201 cgcactgcag aaggaaaaga acgtctgcgc ccggcgcttt ccagcggcaa tctgcaaaaa
     4261 accctgaccg cgcccgtcac cgctatcgtc gcctgggaca gtgaatttta tgaacggtta
     4321 ccactactgt ttccccacgg tgatgcccgc agttggttta cctccagccc acaacttgcc
     4381 gaagaaacag cgtttcgcaa cagttccatg caggcggcct atctgatcgt cgcttgccgt
     4441 gcgctgggac tggataccgg cccgatgtcg ggttttgacc gtcaacacgt tgacgacgcc
     4501 ttttttgcgg gcagcacgct gaagagcaat ctgctgatta atatcggcta tggcgatagc
     4561 agcaaacttt ttgcgcgcct gccacgtctg tcctttgaag aagcctgcgg gctgttgtaa
     4621 ggagcaaact atgaacatag ttgatcaaca aacctttcgc gatgcgatgt cctgtatggg
     4681 cgcggcggtc aatatcatca ccacggacgg tccagccggg cgcgccgggt tcaccgccag
     4741 cgccgtctgc agcgtgaccg acacgccgcc cacattactg gtgtgcctga atcgtggggc
     4801 gtccgtctgg ccggtattca atgaaaatcg aacgctgtgt gtaaatacac tgagcgccgg
     4861 gcaggagccg ctttcaaatc tttttggcgg caaaacgccc atggaacacc gctttgccgc
     4921 cgcccgctgg cagacgagtg tgaccggatg cccgcaactg gaagaggctc tggtttcgtt
     4981 tgactgccgt atcagccagg tggtgagcgt cggcacccac gacattctgt tttgcgccat
     5041 cgaagcgatt catcgtcaca ccacacccta cgggctggtg tggttcgatc gcagttatca
     5101 cgcgctgatg cgccctgctt gttaacctcc ttaaggagac agctgatggc aatgttcggt
     5161 tttcctcact ggcagttaaa atcgacctct acagagagcg gcgtggtcgc gccggatgaa
     5221 cgattgccgt ttgctcagac ggcagtaatg ggcgttcaac acgcggtggc gatgtttggc
     5281 gcgacggtgt taatgccgat tctgatgggg ctggatccca atctttccat tttaatgtcg
     5341 gggatcggca cgttactgtt tttctttatt accggcgggc gcgtccccag ttatctcggc
     5401 tccagcgcgg cttttgtcgg cgtggtgatt gccgcgacgg gctttaacgg tcagggcatt
     5461 aacccgaata tcagcatcgc gctgggcggg attatcgcct gtgggctggt ttataccgtc
     5521 attggcctgg tggtgatgaa aattggtaca cgctggattg aacgactaat gccgccggtg
     5581 gtgacgggtg cggtggtgat ggcgattggc ctgaacttag cgcctatcgc agtgaaaagt
     5641 gtatcggctt cggcctttga tagctggatg gcggtaatga cggtgctgtg tattgggctg
     5701 gtggcggtgt ttacccgtgg catgatccag cggttattga ttctcgtagg attaattgtc
     5761 gcctgtctgc tgtatggcgt gatgaccaac gttttagggc tgggaaaagc cgtcgatttt
     5821 actctcgtca gctatgctgc ctggttcggt ctgccgcatt tttccactcc tggttttaat
     5881 ggccaggcga tgatgttgat tgcgcccgtg gcggtgattc tggtggcgga aaacttaggt
     5941 catctcaagg cggtcgccgg gatgaccgga cgcaatatgg acccgtatat ggggcgagca
     6001 ttcgtcggtg atggtctggc aacgatgctt tccggctctg tcggcggtag cggcgtcacc
     6061 acctatgctg aaaatatcgg tgtgatggcc gtgacaaaag tctactcgac gctggtgttt
     6121 gttgcagctg ccgtcatcgc tatgctgttg ggcttttcgc cgaaatttgg cgcgctgatc
     6181 cataccattc cggccgcggt gattggtggt gcatcaattg ttgtgttcgg gctgattgcc
     6241 gtcgcaggcg caaggatatg ggtacaaaac cgtgtcgatt taagccagaa cggtaatttg
     6301 attatggtcg cagtaacctt cgtgctgggc gcgggtgatt ttgctctcac gcttggcggt
     6361 tttacgttgg gagggatcgg tacagcaacc tttggcgcga ttttactcaa tgcgttatta
     6421 agcagaaggt tggttgacgt tccaccgcca gaagttgttc atcaggagcc ataaacagaa
     6481 aaccgccggg ttaccggcgg tgatgacttt ttgacgagct taagaaaaaa atgcattcgt
     6541 cattgattag cccggctagt tgtcgctttt gccgtgactg ctcttgccac cttttttacc
     6601 tgcttcagat gcgcgctgcg gatcattctt gaaattaccc ccgctgtgct gtccaccttt
     6661 tttacctgct tctgatgctc tttcgcggtc ttctgcaaaa ttgccggaac cgcctcgatg
     6721 gtttgccatt actgacctcc gtctgggtat agacatcata ttgcatagaa ttaacgcggc
     6781 gtatggcggc tgccatattt tcgctacgtg ggactaagct tagtgaactg acgcgggcag
     6841 acccgcgttt ggtaatatcc tgcaacagct tcttagcgag aatagatatc atctccaata
     6901 attatccata agccgctctt atgattaaaa aaacgcgatg agataaaaat gtgtcatttt
     6961 gcgacaaaat tacgtgcttg tgaaagttgt tataaatcaa ataagtggtt gtgaaatttg
     7021 cactctgaaa aggacgtctt atctttaaat aagtggtagc gaatcgctac ggaatagaga
     7081 taacacgagg agtggttaga aatggctaaa gttctggtgc tttattattc catgtacgga
     7141 catattgaaa cgatggcacg cgcagtcgct gagggtgcaa gcaaagtgga tggcgcagaa
     7201 gttgtcgtta agcgtgtacc ggaaaccatg ccgccgcaat tatttgaaaa agcaggcggt
     7261 aaaacgcaaa ctgcaccggt tgcaaccccg caagaactgg ccgattacga cgccattatt
     7321 tttggtacac ctacccgctt tggcaacatg tccggtcaaa tgcgtacctt cctcgaccag
     7381 acgggcggcc tgtgggcttc cggcgcacta tacggaaaac tggcgagcgt ctttagttcc
     7441 accggtactg gcggcggtca ggaacaaacc atcacatcca cctggacgac ccttgcgcat
     7501 cacggcatgg tgattgtccc cattggctac gcagcgcagg aattatttga cgtttcacag
     7561 gttcgcggcg gtacgccgta cggcgcaacc accatcgcag gcggtgacgg ctcacgccag
     7621 ccaagccagg aagaactgtc tattgctcgt tatcaagggg aatatgtcgc aggtctggca
     7681 gttaaactta acggctaatc ttcaacagga ggatacgcat gccaactcaa gaagcgaaag
     7741 ctcatcacgt cggtgaatgg gcatctctgc gcaatacgtc gccggaaata gccgaggcca
     7801 ttttcgaagt cgccgggtac gacgaaaaaa tggcagaaaa gatttgggaa gaaggtagtg
     7861 atgaagtgtt agtcaaagcc tttgctaaga ccgataaaga ttcgcttttt tggggcgaac
     7921 agaccatcga acgtaaaaac gtttaaccag cgactccccc gcttctcgcg ggggagtttt
     7981 ctgttatttc accgcctcat tcaacacgct atcaaactta tccatcgggc agaaaccgtt
     8041 ggcatctatc gggcaaccgc ttaattccag cgtcacgcgc tgcgcaggtg cctgcagggt
     8101 taacgcatcg gcattacgta actgttccgc actctggtac acatattcaa ttttcatcaa
     8161 atcgcgattg gctttgctgt catgccaacg ctggaaaacg attttgccgc caatcggcgt
     8221 gcgttcgttc tggtcatgca actgatacgg tttgaaatcc agcgccgtta acagagaggc
     8281 tatgttggag tcgtgcccaa ctaacaccgt gattttcgga gcactggcgc gatcggtgac
     8341 cagggctttg tcgatataac tgaccagcgg tttcgcaaca ttgcgcgcca cttccggtga
     8401 ggtaaacagg ctgtcctggt aaccgttttt cagcttcgac aataccttcc actgctggtc
     8461 agatttgatt tctccccagg ccacctgatc catcggaaaa ccttcgtagt attgcaaagt
     8521 aaacgcatct accagcgagt tgccgacttt cagcggcccg gaaacacctg gttcttgttg
     8581 atacttggcg ctaaaggtat ttttgccatc caccagcgaa cactgttgtt tctctttaca
     8641 ggcaggggaa tctttatagt taacgatttt ttccagtagc tggtagctgt cggtaagctg
     8701 gagtttgctg agctctttct ccattgccgc caccgcctgt tcactgaatg cggcggaatc
     8761 atcggtgatc atcgggttaa aggttgggtc catagtgccc attttttcct ggtgatgcac
     8821 aggaatatca cacccaggga atgcaccagt aataaagaac tgggcggtag cgacggtacg
     8881 ttgcagacta ttggcatagg cataaacggt gtccggcggc gggcattccc ccgatttcac
     8941 tatcccctgc tctgccagcc attcacgcat gtaatggccc atatacactt cgagcacgcc
     9001 acctttggtg gtgagttgcc caccggggac gtcccattcg ggccatttat tcggcgtcga
     9061 ctgctccagc acactgccat tgttcgccag cggcgcacgt aagttatggc ggctcatcat
     9121 gagcacttgc tgtagctgat agccttccgg tacggtttgt gcctgagcat ttgaagcgag
     9181 taaaactatc cctgccacag ttgcggcgat tagcgttttg ttcattcctg gcacctcttt
     9241 tgttatcaat cattaaagag tgtgacagaa atatgacttt tttccgggaa caggttcgca
     9301 aaatggcttg ttgaacgcct gtttttctat atacatcttc aaaaacaggc aaggtttgac
     9361 gcaaactatg cctgattagc ccctgcctcg cgtacgccgc tgataattgc acctgtggct
     9421 aaacagaata tctgcggcgt taccaataca gagacaatat ttaccaataa aaaagtgtga
     9481 gactaataac aatggttttt agcatatgcc tttgccatta agttgcataa cgatttaaat
     9541 agtcaagttg cgcattcatt tccttatttt ttaacttata agcctgagaa aaaagttcag
     9601 gagggaggta aacatcataa tgattgccag tgtttattaa ataaacatac tcaagatctt
     9661 catctgttga aaaggattca tttcccaagg agattgatgc cacattacta ttaacaataa
     9721 ataatgagca aatgctatct gaaagaacag caagaaaatt cttcaccgct ttaatcctat
     9781 ccgacgtgtc ggtatctaaa gcaagcaaat tcttgttatc attaaatata aactcgggta
     9841 aatgcagtat gcattttttg tcattcaagc aactatcaat gaattgtttt ttaaaaaaca
     9901 gaaagctcca tgacataaaa ctacccacac cctgaatttt gttgtcagat tgcagatcaa
     9961 cgccaaataa tgtctttaca tcttccctga gattatagaa tgccgtatca acaagagcct
    10021 cgatcgtttg tttatcaaca attttaataa actcttcgta tacttgcttt tgcgtcttat
    10081 ggctgcccag cgcccatgaa tactcatgtt cttttaaaac cagtatggct cttaacagac
    10141 aatatccatc gccatctact ggagcgtatt ttaatccata tgttttatta atttcactct
    10201 gaataaaaga tatgccagac aaatcaatac cagtatctaa atcagatatt cccggcatat
    10261 ttatctgttc tggctggata ctcatatttt catcatctaa acaatactca gaaaaaacat
    10321 catcacacgc attaatgttt tcaatatatt ccataaagcc ataccaataa taacctttaa
    10381 aatctgtgat agactctgaa cctctgcccg gcaaggacac tctaacaata tcaacatctt
    10441 gaacatattg tatagtatat gtaatacctc ctgaaacccc tgaataaatt tgtgtatcat
    10501 tagtacgcaa tgttctcata aataatgata gatcattatc attctgcatt tcctcaaatt
    10561 ctttttctag tgattcacat tccagacatg gagtatcctc ttcatttact ttacgtctga
    10621 gttcattgta caaccgcata aaacacacca gtgtgttttc aaaaagcgtg attgaatatg
    10681 tttgattttt ttcgtactca ttttttatat cgttccaggc ctgtttcaaa taattatttg
    10741 cagtgcaact aattccatgt atattactac ccatatatag cgtctataaa atttaataaa
    10801 taatga
//
LOCUS       DBKEKI010000133        10783 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.134, whole genome shotgun sequence.
ACCESSION   DBKEKI010000133 DBKEKI010000000
VERSION     DBKEKI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.134"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            78..854
                     /gene="exuR"
                     /locus_tag="ACUQPX_003380"
     CDS             78..854
                     /gene="exuR"
                     /locus_tag="ACUQPX_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HHV9057969.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1094..1177
                     /gene="yqiM"
                     /locus_tag="ACUQPX_003381"
     CDS             1094..1177
                     /gene="yqiM"
                     /locus_tag="ACUQPX_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HHV9057970.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1199..1861
                     /gene="yqjA"
                     /locus_tag="ACUQPX_003382"
     CDS             1199..1861
                     /gene="yqjA"
                     /locus_tag="ACUQPX_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HHV9057971.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1865..2248
                     /gene="mzrA"
                     /locus_tag="ACUQPX_003383"
     CDS             1865..2248
                     /gene="mzrA"
                     /locus_tag="ACUQPX_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HHV9057972.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2395..2763
                     /gene="yqjC"
                     /locus_tag="ACUQPX_003384"
     CDS             2395..2763
                     /gene="yqjC"
                     /locus_tag="ACUQPX_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HHV9057973.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2801..3106
                     /locus_tag="ACUQPX_003385"
     CDS             2801..3106
                     /locus_tag="ACUQPX_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HHV9057974.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3109..3513
                     /locus_tag="ACUQPX_003386"
     CDS             3109..3513
                     /locus_tag="ACUQPX_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HHV9057975.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3503..3802
                     /locus_tag="ACUQPX_003387"
     CDS             3503..3802
                     /locus_tag="ACUQPX_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HHV9057976.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3988..4380
                     /locus_tag="ACUQPX_003388"
     CDS             3988..4380
                     /locus_tag="ACUQPX_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HHV9057977.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4450..5436
                     /gene="yqjG"
                     /locus_tag="ACUQPX_003389"
     CDS             4450..5436
                     /gene="yqjG"
                     /locus_tag="ACUQPX_003389"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HHV9057978.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5729..6097
                     /locus_tag="ACUQPX_003390"
     CDS             5729..6097
                     /locus_tag="ACUQPX_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HHV9057979.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6304..7200)
                     /gene="yhaJ"
                     /locus_tag="ACUQPX_003391"
     CDS             complement(6304..7200)
                     /gene="yhaJ"
                     /locus_tag="ACUQPX_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HHV9057980.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7305..8006
                     /locus_tag="ACUQPX_003392"
     CDS             7305..8006
                     /locus_tag="ACUQPX_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HHV9057981.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            8029..8193
                     /gene="yhaL"
                     /locus_tag="ACUQPX_003393"
     CDS             8029..8193
                     /gene="yhaL"
                     /locus_tag="ACUQPX_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HHV9057982.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8405..9714)
                     /gene="cyuA"
                     /locus_tag="ACUQPX_003394"
                     /pseudo
     CDS             complement(8405..9714)
                     /gene="cyuA"
                     /locus_tag="ACUQPX_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
     gene            complement(9742..>10783)
                     /locus_tag="ACUQPX_003395"
     CDS             complement(9742..>10783)
                     /locus_tag="ACUQPX_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9057983.1"
                     /translation="DYPSVISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASY
                     LHTFGVTEGLLSDSPFYGLVLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSM
                     VGMWHLYNVGSLPPLGLLVKNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARH
                     KALRAMNIAFGILFVTVFFYAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAW
                     VKVVSVILNIFAVMTAFFGVYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFA
                     ILLAWSAIVLNAPVLSFTSICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTG
                     LLLCVSLFLAFS"
BASE COUNT         2719 a         2841 c         2675 g         2548 t
ORIGIN      
        1 tttatatatg accaatggca tacccatgaa taaagtggta tgataacttc gtccgcattc
       61 cactccggga gacgcatatg gaaatcactg aaccacgccg tttgtatcaa caacttgctg
      121 ctgacctgaa agagcgcatt gaacagggcg tctatctggt gggtgataaa ctgcctgccg
      181 aacgctttat tgccgatgaa aagaacgtca gccgaacggt agttcgtgaa gccatcatca
      241 tgctggaagt tgaaggctat gtggaggtgc gtaaaggttc aggtattcat gtggtttcca
      301 accagccgcg ccatcagcag gcggctgaca ataatatgga attcgccaat tacggtccgt
      361 ttgagttgct tcaggctcgc cagctcatcg aaagtaatat tgccgagttc gcggcaactc
      421 aggtaacgaa acaagacatc atgaaactga tggccattca ggaacaggcg cgcggcgaac
      481 aatgcttccg tgattccgag tgggatttac agttccacat tcaggtagcc ctggcgacgc
      541 agaactccgc cctggcggcc atcgttgaaa aaatgtggac ccagcgtagc cataacccgt
      601 actggaaaaa actacacgaa cacattgatt cccgtaccgt tgataactgg tgtgatgacc
      661 acgatcaaat tctcaaggcg ttaattcgca aagatcctca tgctgctaag ctggcaatgt
      721 ggcagcatct ggaaaacacc aaaatcatgt tatttaacga aaccagcgac gatttcgagt
      781 tcaatgccga ccgctatctg ttcgccgaaa acccggtagt gcatctcgat acggccacca
      841 gcggcagcaa atgaaattcc tgtccgacag gcgctgcaat gcgcctgttt gtctcgtcgg
      901 taagcgaaaa gtataaagtg tcagcctgtg taaatcctct cgccaccctc ccctgcaatc
      961 agcaaaatca gactccacgg acatgtaatt ttgataacga acaacgttga cctttgttac
     1021 aattagattc aatttgaatt tatgcttttg aatgctttct tatctcacgt tttaacaggg
     1081 aatagttcag gctgtgttga tgtatcaaac ccgcagaaca taccaaaaca gcaataacat
     1141 tgcggtagtg catcttttaa aaccagcgtg gcgttaaccg attcaccagg aataatgaat
     1201 ggaacttttg acccaattgc tgcaagccct gtgggcgcag gattttgaaa ccctggccaa
     1261 tccatcgatg attggcatgt tgtattttgt cttgtttgta attttgttcc ttgaaaatgg
     1321 cttgcttccg gcggcctttt taccgggcga cagtttactg gtattggtcg gcgtgttgat
     1381 tgcgaaaggc gcgatgggtt acccgcaaac gattctgctg ctgaccgttg ccgccagcct
     1441 cggttgctgg gtcagctata ttcaggggcg atggctgggc aatacccgca ccgtacaaaa
     1501 ctggctatct catttacccg cgcattatca tcaacgcgca caccatcttt ttcataaaca
     1561 cggtttatcg gcgctgttaa ttggtcgctt tattgcattt gtcagaacac tgctgccgac
     1621 gattgccggg ttatcagggc tgaataacgc gcgctttcag ttcttcaact ggatgagcgg
     1681 tctgctgtgg gtattgatcc tgacaactct gggttacatg ctcggcaaaa cgccggtatt
     1741 tttaaagtac gaggaccagc tgatgtcatg cctgatgctg ctcccggtgg tgctgctggt
     1801 gtttggcctg gcaggttctc tggtcgtgtt atggaaaaag aaatatggaa atcgggggta
     1861 agggatgcaa atacctcgca tgtcgcttcg ccagctagcc tggtccggca ctgttttact
     1921 tctggtcggc acgctgttgc tggcctggtc cgcggttcgc cagcaagagt ctacgctggc
     1981 gattcgtgcc gttcatcaag gcacaacgat gccagacggt ttttcgatct ggcatcacct
     2041 tgacgctcat ggcattcctt tcaaaagtat cacccccaaa aacgacactc tgttaattac
     2101 atttgactcc agcgaccaga gcgccgccgc aaaagcggtc ctcgacagaa cattgcccca
     2161 tggctacatc attgcgcagc aggacaataa cagtcaggct atgcagtggc tgacccggtt
     2221 acgggataac tctcatcgct tcggataact tcctgttatc cgaaacatat cactcacttt
     2281 ggtgatttca ccgtaactgt ctatgattaa tgaggcggtg gttaacacta ccgcttcgtt
     2341 gtctggacaa tcgttccttt gtaataggtc cgaatcacaa tggaaggttc aagaatgaaa
     2401 taccgcatcg ctttagctgt ttctctcttt gctcttagtg ccggtagtta tgccactacc
     2461 ctgtgtcagg aaaaggagca aaatatcctt aaggagatca gctatgccga aaaacaccaa
     2521 aaccagaatc gtattgacgg tctgaataaa gccctgagtg aagtccgggc caactgttca
     2581 gatagccagc tgcgtgccga tcatcacaag aaaatcgcaa agcagaaaga tgaggtggcg
     2641 gaacgccagc aagatttagc cgaggcgaag caaaaaggcg atgccgataa gattgccaaa
     2701 cgcgaacgga aactggcaga agcgcaggaa gagctgaaaa agctggaagc gcgcgactac
     2761 taactcacaa tagtcactac ttactcacct ggagaaaact atgtcgaaag aacacactac
     2821 ggaacatctg cgtgctgagt tgaaatccct ttccgatacg ctggaagagg tgcttagctc
     2881 atctggcgag aagtcgaaag aagagttgag taagattcgt agcaaagcgg agcaggcact
     2941 gaaacagagc cgttatcgcc tgggtgaaac cggtgatgcc atcgccaaac aaacccgtgt
     3001 cgcggcggcg cgtgctgatg agtatgtgcg tgaaaatccg tggaccggcg tgggaattgg
     3061 cgctgcaatc ggtgtagtgc tcggcgttct gctgtcgcgt cgttaattat ggcggacact
     3121 catcacgcgc aagggcccgg taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc
     3181 atggttgaaa tggtggaaac acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa
     3241 gctaatctct ttcaactttt actgatgctg ggcctgacga tgcttttcgc tgcatttggt
     3301 cttatgagcc tgatggtgct aattatttgg gcggttgatc cgcaatatcg cctgaatgcg
     3361 atgattgcca ccaccgtggt gttgctgcta ctggcactga ttggcggtat ctggacgtta
     3421 cgtaaatcgc gtaaatccac cttgctgcgc catacacgcc atgagctggc aaacgatcgg
     3481 caactgctcg aggaggagtc ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca
     3541 actgcttagc cagatccagc aacaacggct ggatctttcc gccagtcgtc gtgaatggct
     3601 ggaggcaaca ggcgcttacg atcgtcgctg gaatatgctg ctaagtctgc gctcctgggc
     3661 gctggttggc agtagcgtga tggcgatctg gacgattcgc catcctaata tgctggtccg
     3721 ctgggccaga cgcggttttg gcgtatggag cgcctggcgt ctggttaaaa cgaccctcaa
     3781 gcagcaacag cttcgcggtt aacttccctc tggccggagc aattccggcc ttatccctca
     3841 aattttttga atatttttga cagttttcct tgctaacaat catcattcac cacgtttatg
     3901 attctctcca tcgacagcaa cgacgctaat accgcgccct tgcacaaaaa aacaatcagc
     3961 agcctgagtg gcgcagtgga gagtatgatg aaaaaattag aagatgttgg tgtactggta
     4021 gcgcacattt taatgccgat tctgtttatt accgcaggct ggggaaaaat caccgcttac
     4081 tctggtaccc aacaatatat ggaagcaatg ggtgtcccgg gttttatgct gccgctggtg
     4141 attctgcttg agttcggtgg tggtctggca atcctgttcg gtttcctgac tcgcaccaca
     4201 gccctgttta ctgcgggctt tacgctgctg acggcatttt tatttcacag caactttgct
     4261 gaaggcgtca actcgctgat gttcatgaaa aacctgacaa tttctggcgg attcctgctg
     4321 ctggcaatta ccggtccggg cgcgtatagc atcgaccgcc tgctgaataa aaagtggtaa
     4381 gcacgcgcta tacttaacga taaaaaacga ggaggaagct cctcgttttt gctattggag
     4441 gagagaaaaa tgggtcaact gattgacggc gtctggcatg acacctggta cgataccaaa
     4501 tctaccggcg gtaaatttca acgttcagct tccgcatttc gtaactggct cactgccgat
     4561 ggcgcacctg gccccactgg cacaggcggt tttatcgcag agaaagatcg ttatcatctc
     4621 tatgtttcac tcgcctgccc gtgggcgcac cgcacgctga tcatgcgcaa actcaaagga
     4681 ctggaaccgt ttatttccgt ttccgtagtg aacccgctga tgctggaaaa cggctggacc
     4741 tttgatgaca gttttccggg agcaaccgga gatacgctct atcaacatga atttctgtat
     4801 cagctttatc tccacgccga tccacactac agcggacgag ttactgttcc cgtgctgtgg
     4861 gacaaaaaga accacaccat cgtcagcaac gaatcagcag aaatcatccg catgttcaat
     4921 accgcgtttg atgcgctggg cgcaaaagcg ggtgattact acccaccagc cctgcaaacg
     4981 aaaattgacg aacttaacgg ctggatttat gacaccgtta acaacggcgt gtataaagct
     5041 ggttttgcca ccagccagca agcctacgac gaggcggtgg cgaaagtgtt tgaatcgctg
     5101 gcgcgactgg agcagatttt aggccagcat cgttacctga ccggcaacca gctaaccgaa
     5161 gccgatattc gcctgtggac tacgctggtg cgttttgatc cagtgtatgt gacccacttc
     5221 aagtgtgata agcaccgcat cagcgattac ctgaatctgt atggcttcct gcgcgatatc
     5281 taccagatgc cggggatcgc cgaaactgtc aatttcgatc acatccgtaa tcattacttc
     5341 cgcagccata agaccatcaa ccctacgggg attatttcaa ttggtccgtg gcaggatctc
     5401 gatgaaccgc atggacgaga tgttcgcttc ggttaaaaaa aggcacctta cgaggtgcct
     5461 ctgataatta gctaagtgca tgttgttcat gtcggatgcg gcgtgaacgc cttatcaaac
     5521 ctacaaagcg tttatattca atatgttaca atgaccgcgt aggccagata agcgtagcgc
     5581 atctggcgat tttgcgcttg ccatcagtct tagcgcgcct tcacggcact ttattaattc
     5641 cttgaacgaa tatttactgc catttcattc acgtctattc ttaatttgct gctttaaaaa
     5701 caagtgattg agcaaaattg aggcaaaaat ggactggtat ctgaaagtac taaaaaatta
     5761 tgtcggtttc cggggtcgtg cgcggcgcaa agagtactgg atgtttattc tggtcaacat
     5821 catctttacg ttcgtgctgg ggctgctgga taaaatgtta ggctggcaac gcgctggtgg
     5881 cgaaggcatc ctgacgacaa tctacggtat tctggtgttt ttaccgtggt gggcggttca
     5941 gttccgccgc ctgcacgaca ccgaccgctc ggcgtggtgg gcactgctat tcttaatccc
     6001 gtttatcggc tggcttatta tcatcgtctt taactgccag gcgggtacgc caggcgaaaa
     6061 ccgctttgga ccagatccaa aactggaaca agaataatga ttaagcacct tgtaaaaggt
     6121 gcttcaaatt gttgacaaag tgcgctttgt ttatgccgga tgcggcgtaa acgccttatc
     6181 cagcctacaa attgccaaaa ttcaatatat tgcagtgact gcgtaggcct gataagcgta
     6241 gcgcatcagg cagttttgcg tttgtcagca gtcagaagca ccttgtgaaa ggtgcttaat
     6301 agattatttt ccagcgaaca actttggaat ttcccgcaga caccaggatt tcgcttcccc
     6361 catactgtca cgacgccagg ccataataat atcgatctcg ctggtcgatt ccgggctgac
     6421 gacacgcaac cgcccttccg caatatcttt ttcgaccatc ggatacggca tcgtcgccac
     6481 gccaagcccc gccagtaatg cctgacgttt atcttcaatc gtgctcaccg ttaagcgcgg
     6541 ctgtttgtcc agcagctgta cggtcaacac cgggcgctca cgagcggtat ccgctaccgc
     6601 aattccacga tatttcacac gcgtcacttc agataacggt tccggctcct gatgaatcgg
     6661 gtgatcaggc gcggcaacgt agacgttcat taacgtatag agcttgcgcg agttgatctc
     6721 cgacgaggaa cgaaaatgca tatccggcgc gataacgata tccgcccgcc cctgctccag
     6781 ccgttcccac gcccccgcca gcacttctgt gatgattgcc agttgggtat tggcttttgc
     6841 cgccagtttg tcgattaacg ggaaaaaggc aggtgtcggt accagcgctt cggtcacaat
     6901 ggtgagatgc gtttcccaac cgcgcgcgag agcttccgca tcagtagtca gtttatctgc
     6961 ggcttccagc aaaacgcgcc ctcgctccag caacatcctc ccgacattgg tgaatttggt
     7021 gcgatggccc gagcggtcaa acagcaccac atccagctct tcttccagtt tttgcatggt
     7081 gtagctaagt gcggaaggca cgcgtcccag ctcatccgct gccgccgcaa aactgccccg
     7141 gcgatcgatc gcatccataa cccgtagtgc ttccagcgtt aatgcccttt ctttggccat
     7201 ttcgttctca ttcaggaaat ttgaacatac cgggcagaat atctggctaa caatgtagcg
     7261 tccagcccct taccataaaa ggaagtaaag agaggtcaag aattatgatt actacccgaa
     7321 ctgccaggca gtgtggacaa gcagactacg gatggttgca ggcccggtat actttttcct
     7381 ttggacacta cttcgacccg aaattgttag gctatgcctc cctgcgtgtg cttaaccagg
     7441 aagtgctggc cccaggtgcc gcctttcagc cgcgaaccta tcccaaagtc gatattttaa
     7501 atgtgattct ggatggagaa gcagagtatc gcgacagcga aggcaatcat gttcaggcca
     7561 gcgccggaga ggcgttgctg ctctctaccc agccgggtgt cagctatagc gaacacaatc
     7621 tcagcaaaga caaaccgtta acgcgaatgc agctttggct ggatacctgc ccgcagcgag
     7681 agaatccgct gattcaaaag ctggcgctta atatggacaa gcagcaatta atcgcctcgc
     7741 cagagggtgc gatgggaagc ctgcaattac gccaacaagt gtggctgcac catatcgtgc
     7801 tcgacaaagg cgaaagtgcg aatttccagt tgcatgggcc acgcgcgtat ttgcaatcga
     7861 ttcacgggaa atttcatgcg cttacgcatc atgaagagaa agcggcgcta acctgcggtg
     7921 atggggcgtt tattcgtgac gaggctaaca ttacgctggt tgccgattcc ccactgcgcg
     7981 ctttgctgat agatttgcct gtctagttgt ttttacaggg agatgatgat gagtaaaaaa
     8041 tcgaccaaaa agcgccagcc agcgaagccc gtggtggcga aagaacctgc tcgcaccgcc
     8101 aaaaattttg gctatgaaga gatgttgagc gagctggaag ctatcgtcgc ggatgctgaa
     8161 acgcgtttag ccgaggatga agctaccgcg taaagacagg ttgccggatg cggcgtaaac
     8221 gccttatccg gcctacaaaa cacaacaaca tcaacaaatt aaacaccccg taggccggat
     8281 aagatgcgcc agcatcgcat ccggcatcag gtacaaaaca caacaacatc aacaagttga
     8341 gcaccccgta ggccggataa ggtgcgccag catcgcatcc ggcacaatac tcaaaacctg
     8401 gcgtttatct ggccttgctc gccataatct cgataatctg ccgatccgtt tgctgcatcg
     8461 aatggcttgc taacgcacac aggttggcaa tcgactgctc aacaccatgc gccacgatcc
     8521 cttcattgcc ggtcacggcg gtatcatcca gcgccattaa caccgctttc cacgcagccg
     8581 aagcactggt cgaaaccttc atcgcacagc tgttcgacgc accatcgcaa atcatgccgc
     8641 tgacatcgcc gatcatactg ctgatcgcca tcgagatagt ttcataacgc ccatccacca
     8701 gccatgccat cccggcggcg gccccattgc tgcggtcgtt gcggcacaaa gtgcagacaa
     8761 acgcggtaac tggttatgga tgtaaattgc gctcaaatga gaaagcatca gcgcacgcgc
     8821 cagtcgttca tcatccgctc cgaagtgttc tgctacaacc accacaggca ttgttgcggt
     8881 gatcccctgg ttacccgagc cggagttact catagccgga agcgtagcgc cgcccatacg
     8941 cgcatcggat gccgcgctgg tacgaatcac aatggatgaa gagagatctt tcgccagcaa
     9001 accgcgctcg cactgttttt ccagcgtcgc gccaatatgc agcccccact taccgctcaa
     9061 accttcctgc gataacgcac aatttagctt cgcggaatcg agaataaagc ggatcgccgc
     9121 aaacgggagt tcattgacga acttcaggat ctcagccagc gtcgttctgg aaagcaccgt
     9181 cagcggagac tcttgctcgc cctctgccac acacgcctgc tgggtaaaca ccacaccatt
     9241 gtgcgtctca atatgcacaa tgttggtatg cccgccgacg atggtgacac acgcccactt
     9301 ctcaccgttc cagactttgg cgcgtgagaa gaggatttca ttgcaaggtt cctggatctt
     9361 aacagagact ttccccgccg ccagcagtgc tttggcatcg gcaattgcct gcgcagttgc
     9421 gtctttcagc acttccagcc cggcgttggc atttccacct aacgccccca gcgccgccgc
     9481 aatcggcagc cccaccattc ccgtgccggg aacggtgacg cccagaccgt tcttcatcag
     9541 atttggcgaa acccaggctt ctacacgttc aaccggacct tccagttctg ccgcagcaac
     9601 cgccgccgcc agcgccagtg aaatcggttc agtacatccc agcgccggtt ttacttcctc
     9661 ctgaacggcg aggatgtaac gctgccataa cggatttaaa gtcgaatcaa acatattaaa
     9721 aaccttaaaa tttcaggtaa atcaagaaaa tgccaggaac agagaaacac aaagcaacaa
     9781 accagtgacg ataatcaggt acagagacat ccctttgtat ttgtgcaatg ccggtacttt
     9841 gtaaaccagc cacgccggga tcaggcaccc taccatgccg aaaatcgggc tacagataga
     9901 ggtgaagctc aacaccggtg cgttcagtac gatggcgctc caggccagca aaatggcgaa
     9961 aatcatgatg ccgcgctgaa caagattttc gttaatcttc tcggcaggca tcttgcgacg
    10021 caggatgttc attacgatcc cttgcgttgc ttcgcgaaag cctaaataga cgccaaagaa
    10081 tgcagtcatg acagcaaaga tattgagaat gacgctgacc actttcaccc acgctgcgcc
    10141 gtcaccgcta ataaactgtg cggcaatcgc cagcgcggaa atattctgct cgtaggcttt
    10201 taccgcttcg tcatgtccca tcgccagcgt gaacgacacg gcgtagaaaa agacggtgac
    10261 aaacaaaatg ccaaacgcga tattcattgc ccgcaatgct ttatgccgcg ccacttcaat
    10321 tgatttttcc cgcgagcgat aagagatcac catcgggctt aacgtctgga taaacagaat
    10381 cgacgtcagg gtaaacggca gcgtaataat ggcgtttttc accagcagcc ccagcggcgg
    10441 tagcgaaccg acgttataca gatgccacat tcccaccatc gacacgccca gcgcagcgac
    10501 taccagcagc ttggtcagca ccatgccggt cgaaattttg aataacaatt tctcgccgcg
    10561 tgaagagatc gccaccagaa tgcaaatcag taccagacca taaaagggac tgtctgacag
    10621 caacccttcc gtcacgccga aggtatgcag ataggaagca ctatcgttgg tgatggcggt
    10681 ggaatagacg aacatccaaa tcaccagcat tacgaaatag agcgcaccta acaggatgcc
    10741 ccagttttta cctaaataac cgctaatgac actcgggtaa tct
//
LOCUS       DBKEKI010000134        10764 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.135, whole genome shotgun sequence.
ACCESSION   DBKEKI010000134 DBKEKI010000000
VERSION     DBKEKI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.135"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACUQPX_003396"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACUQPX_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HHV9057984.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACUQPX_003397"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACUQPX_003397"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HHV9057985.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRINMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACUQPX_003398"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACUQPX_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HHV9057986.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDGLTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGVGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACUQPX_003399"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACUQPX_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HHV9057987.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACUQPX_003400"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACUQPX_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACUQPX_003401"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACUQPX_003401"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HHV9057988.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACUQPX_003402"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACUQPX_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HHV9057989.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNMETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACUQPX_003403"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACUQPX_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HHV9057990.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWISLTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGIAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACUQPX_003404"
     CDS             complement(9457..10149)
                     /locus_tag="ACUQPX_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HHV9057991.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLMAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            10629..>10764
                     /locus_tag="ACUQPX_003405"
     rRNA            10629..>10764
                     /locus_tag="ACUQPX_003405"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2662 a         2584 c         2835 g         2683 t
ORIGIN      
        1 aagggggggg ctttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctact aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg gtgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgctg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgttgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccactgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtac tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag cgggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgccagacgc ctcctttctt cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaagcc gcggatcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcaata tggcattaac ccaaggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg cagcgttaaa tgtctatccg ggccgtgtga
     2701 tggcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct
     3001 ttaaaagcga caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgggctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 caggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgta ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgct gatgaacagc
     3721 aggctgtgag tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt attgatcctt gatgagccta cccgtggagt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagtatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cttcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatag acttgtcggt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcac gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgtccaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggctgggat cattgaagtg gcgcgtctct cctctgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt gggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacattccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 ggtgaagtgg tgagccacat tgcatctgat aacgtactgg gcggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgccggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcaccaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgttg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtactggc gatgacagca ttggcgagag cgttcgccag cagctcgcca ctgataacat
     6361 cgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggcgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ctctttcccc
     6481 atcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcgttat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaag ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc agccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgtttggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagctctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaata tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttactgtt actgtgtacc gaaacgcatc agccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc taccgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgctgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggcata
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa actgcgtcga catagctatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaatgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg gtacacagcc ggtccccaga cgggttgcaa tattgcctac gataccgatc
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt acgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggaaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg aaaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattaattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatacc ggaagaagtt cattacgagg ataagcacgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccttg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caacgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgacat actacgcttc
     9421 tttttatcgc tcattcaatg tcctgttatc gggttatcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg ataatcgcat cgacaatcgc
     9541 ctggtgcaga tccagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccattaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccgggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gcacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aattccgaca gagaaagcaa aaataaatgc ttgactctgt agcgggaaag
    10441 cgtattatgc acaccccgcg ccgctgagaa aaagcgaagc gcactgctct ttaacaattt
    10501 atcagacaat ctgtgtgggc actcgaagat acggattctt aacgtcgcaa gacgaaaaat
    10561 gaataccaag tctcaagagt gaacacgtaa ttcattacga agtttaattc tttgagcatc
    10621 aaacttttaa attgaagagt ttgatcatgg ctcagattga acgctggcgg caggcctaac
    10681 acatgcaagt cgaacggtaa caggaagcag cttgctgttt cgctgacgag tggcggacgg
    10741 gtgagtaatg tctgggaaac tgcc
//
LOCUS       DBKEKI010000135        10740 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.136, whole genome shotgun sequence.
ACCESSION   DBKEKI010000135 DBKEKI010000000
VERSION     DBKEKI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.136"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..913)
                     /locus_tag="ACUQPX_003406"
                     /pseudo
     CDS             complement(1..913)
                     /locus_tag="ACUQPX_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1185..1279)
                     /locus_tag="ACUQPX_003407"
     tRNA            complement(1185..1279)
                     /locus_tag="ACUQPX_003407"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1243..1245),aa:Sec,seq:tca)
     gene            1572..2955
                     /locus_tag="ACUQPX_003408"
                     /pseudo
     CDS             1572..2955
                     /locus_tag="ACUQPX_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            2965..5283
                     /gene="yicI"
                     /locus_tag="ACUQPX_003409"
     CDS             2965..5283
                     /gene="yicI"
                     /locus_tag="ACUQPX_003409"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HHV9057992.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(5336..7045)
                     /locus_tag="ACUQPX_003410"
     CDS             complement(5336..7045)
                     /locus_tag="ACUQPX_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HHV9057993.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTSLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(7166..8557)
                     /gene="xanP"
                     /locus_tag="ACUQPX_003411"
     CDS             complement(7166..8557)
                     /gene="xanP"
                     /locus_tag="ACUQPX_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HHV9057994.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            8837..10042
                     /gene="gltS"
                     /locus_tag="ACUQPX_003412"
     CDS             8837..10042
                     /gene="gltS"
                     /locus_tag="ACUQPX_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HHV9057995.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(10076..>10740)
                     /locus_tag="ACUQPX_003413"
     CDS             complement(10076..>10740)
                     /locus_tag="ACUQPX_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HHV9057996.1"
                     /translation="RVRHACITEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPE
                     LNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMIIENPERLGL
                     AQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPG
                     ELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYS
                     NA"
BASE COUNT         2501 a         2691 c         2833 g         2715 t
ORIGIN      
        1 atgatgtttt gagattggtt caagctgctt aaatagcata atgacctgct cagagagcgg
       61 cacaatatga ggtcgcttca tcttcattac ttctgccggg atctcccaca attttgtctc
      121 aaaatcgata tcttcccagc gtgcaaaacg cagttcttgt gttcgtacgc cggttaacat
      181 aatgatctga gtagctgtct tagtgattat acttccggta taacccgcca gatcgttgag
      241 gaagtgggga agttcattag cagtaagaaa gggaaaatgt acttttttcg gtgtagctaa
      301 agcactggca agatcggggg ctgggttata gtctgcccgg ccagtaacaa ttgcatagcg
      361 gaacacctca ccacagcgct ggcggacctt gcgcattttc tccagagctc cacgtttttc
      421 cattttacgc agtgcttcca gtaactccat tggtttgatt tcggcaatag ggcgcttgcc
      481 aatgtaggga aaaatgtctt tttcaaaggt atcaatgatt tcatcacgat agcgtaatga
      541 ccaccgatca gcttttaact ggtgccattc gcgagcaata gcctcgaacg tatttttcag
      601 actcatttgc tgcgcgattt tttcttcctt ttttgcctcc cctggatcgc ctccagcagc
      661 gaggagtttc ctggcttcac tgcgtttctc gcgagcatcg gctagcgtta catcggggta
      721 tacgccaata gaaagcatct tttctttacc agcaaagcgg tacttcatgc gccagtattt
      781 agaaccatta gtgttaatca gtagatacat accgccgcca tcagcaagct tatagggttt
      841 gtctttgggt tttgcggttt tcactttgat atcggttaat gccatgacat cctccggctt
      901 ttgatgaatt catgttcctg agggggattt ctgccagtaa ctaacctgat acccccaata
      961 caccccttgt ataacaaaga tattgttaaa agccgttgtt atgtatcaca tttttcctat
     1021 tatgggggtg cttgtatttc aataggaaat aacaccccct aaagtacccc caaaagaaac
     1081 ttgatacggg gtgaagtagg ttgacctcag gatacaaata aatactgata tacaagggat
     1141 tagaagagat ttattgacat tgggagagtt cagaagaggt aaactggcgg aagatcacag
     1201 gagtcgaacc tgcccgggac cgctggcggc cccaactgga tttgaagtcc agccgcctca
     1261 ccggagacga cgatcttccg cgcctcgatt gctacatgga ggcggggcgc attatagcta
     1321 cttccttgag tttctacatc ccccagatca gttttttccg cctaataaac gcagcgtatt
     1381 atcacgaaag cattataatt catataaaac ccatatttta caatgatata tgagcggaat
     1441 taacgctacc acgatcacat aaaacaagaa tcataaatta ataaccagat atcggaatat
     1501 tcgctctcac agggatggtt tacaaaatgc gttatcggtc tcagcacccc tatagcatca
     1561 aggaaaagca gatgaagagt gaagtgttgt ccgttaaaga gaaaattggt tatggcatgg
     1621 gagacgccgc cagccacatt attttcgata acgtaatgtt atatatgatg ttcttttata
     1681 ccgatatttt tggcattcct gccggatttg tcggaaccat gtttttggtc gctcgtgcac
     1741 tggatgcgat ttccgatcct tgcatggggt tgttggccga tcgaacgcgc tctcgctggg
     1801 gtaaatttcg tccgtgggta ctgtttggcg cactgccatt cgggatcgtc tgtgtactgg
     1861 cctatagcac gccagatctc agtatgaacg gcaaaatgat ctatgcagca attacttaca
     1921 ccctaactta ccttacttta taccgtcgtc aatatccctt actgcgcatt gggtggtgta
     1981 atcaccaatg acccgactca gcgtatctcg ctgcaatcct ggcgttttgt gctggcgacc
     2041 gcgggaggca tgctttctac tgttctgatg atgccactgg ttaatttaat tggcggtgat
     2101 aataaaccac tcggtttcca gggcggtatc gtggtccttt ccgtggtggc attcatgatg
     2161 ctggcatttt gtttcttcac cactaaagaa cgcgttgaag caccacctac aacaacgtct
     2221 atgcgggaag atttacgtga tatctggcaa aacgaccagt ggcggattgt cggtttacta
     2281 accattttca atatcctagc ggtgtgcgta cgcggtgggg cgatgatgta ttacgtcaca
     2341 tggattttgg gcacgccgga agtgtttgtc gcttttctca ccacttattg cgtgggtaac
     2401 ctgattggtt ccgcactggc aaaacctctg accgactgga aatgtaaagt cactatcttc
     2461 tggtggacga acgccctgct ggcagtgatt agcctcgcga tgttctttgt tcccatgcag
     2521 gccagcatca ctatgtttgt cttcatcttc gtgattggtg tgttgcatca actggtgaca
     2581 cctatccagt gggtaatgat gtccgatacc gtcgactacg gcgagtggtg caatggtaaa
     2641 cgcctgaccg ggatcagttt tgctggcacg ctgtttgtgc tcaaactggg gttggccttc
     2701 ggcggcgctc ttatcggctg gatgctggct tatggcggat atgatgcggc agaaaaagcg
     2761 cagaacagcg ccacgattag catcattatt gcgctattca cgattgttcc ggcgatctgt
     2821 tatttgctga gcgcgattat cgctaaacgc tactactcac tcacgacgca caatctgaaa
     2881 accgttatgg aacagctggc tcagggtaaa cgccgttgcc agcaacaatt cacctctcaa
     2941 gaagtgcaga actaaggaac ggcaatgaaa attagcgatg gaaactggtt gattcaacct
     3001 ggcctcaatt tgattcaccc gcttcaggta ttcgaggttg aacagcagga taatgaaatg
     3061 gtggtctatg ctgccccccg tgatgtgcgt gaacgtacct ggcagcttga tacgccttta
     3121 tttacgctgc gctttttctc cccacaggaa ggtattgtcg gtgtgcggat tgagcatttt
     3181 cagggggcgc tgaataacgg tcctcattat ccgctcaata ttttgcagga tgtgaaggtc
     3241 acaatcgaaa acacagaacg ttatgctgag tttaaaagtg gcaacttaag cgcgcgtgtc
     3301 agcaaaggtg agttctggtc actggatttt ctgcgcaacg gcgaacgtat taccggtagt
     3361 caggtgaaaa ataatggcta cgtgcaggac acgaataatc aacgcaatta tatgtttgag
     3421 cggcttgatc ttggcgttgg cgaaacagtt tacggtctgg gagagcgctt tactgccctg
     3481 gtgcgcaatg gccagacggt agagacctgg aaccgggacg gcggcacaag tactgaacag
     3541 gcgtataaaa atatcccgtt ctacatgact aaccgtggtt atggggtact ggtcaatcat
     3601 ccccagtgtg tctcttttga agtgggatcg gagaaagtct ccaaagtgca gttcagcgtt
     3661 gagagtgaat atctcgaata ctttgttatc gacggcccga cgccgaaagc ggtacttgat
     3721 cgttataccc gctttactgg tcgtccggcg ctgccgcccc cgtggtcctt cggcctgtgg
     3781 ctaaccactt catttaccac caactacgac gaagcgacgg taaacagctt tatcgatggt
     3841 atggcggaac gcaatctgcc gctgcatgtt ttccactttg actgtttctg gatgaaagcc
     3901 ttccagtggt gcgattttga gtgggacccg ctgactttcc ctgacccgga agggatgatc
     3961 cgccgcctga aagcgaaagg gctgaaaatc tgcgtctgga ttaaccccta tatcggtcaa
     4021 aaatcccccg tctttaaaga gttacaagag aaaggctatt tactcaaacg cccggacggt
     4081 tcgctatggc agtgggataa atggcagcca ggtctggcga tttatgactt taccaatccg
     4141 gatgcctgca aatggtacgc cgacaaactg aaaggtctgg tcgcgatggg cgttgattgc
     4201 tttaagaccg actttggcga acgtatccca actgatgttc agtggtttga cggttccgat
     4261 ccgcagaaaa tgcataacca ttatgcgtac atctacaacg aactggtgtg gaacgtgctc
     4321 aaggacaccg ttggtgagga agaagctgtc ttgtttgccc gctcggcctc cgtcggtgcg
     4381 cagaaattcc cggtacactg gggtggcgat tgttacgcta actacgaatc aatggcggaa
     4441 agcctgcgcg gtggtttgtc tattggcctt tcaggttttg gcttctggag ccacgatatc
     4501 ggcggctttg aaaataccgc tccggcgcac gtttacaaac gctggtgcgc gtttggtttg
     4561 ctctccagcc atagccgttt acacggtagc aaatcttatc gtgtgccgtg ggcctacgat
     4621 gatgagtcct gtgatgtggt gcgcttcttc acgcaactga aatgccgcat gatgccgtat
     4681 ctgtatcgtg aagctgcgcg tgcgaacgcg cggggtacgc cgatgatgcg ggccatgatg
     4741 atggagttcc cggacgatcc ggcttgtgat taccttgacc gtcaatacat gttaggcgac
     4801 aacgtgatgg ttgcgccggt gttcactgaa gcgggcgatg tgcagttcta cctgccggaa
     4861 ggtcgctgga cacacctgtg gcacaacgat gaactcgacg gtagtcgctg gcataaacag
     4921 cagcacggct tcctgagtct gcccgtttat gtgcgtgata acactctact ggcgctgggc
     4981 aacaacgatc aacgtcccga ttacgtgtgg cacgaaggca cggcattcca cctctttaat
     5041 ctgcaagacg ggcatgaagc cgtctgtgaa gtgcccgctg ctgacggatc ggtgatcttt
     5101 actttaaaag cagcacgtac tggcaacacg attactgtga ctggtgcggg cgaggcgaag
     5161 aactggacgc tgtgcttgcg caatgttgtg aaagtaaatg gtctgcaagg cggttcgcag
     5221 gctgaaagtg agcaggggct ggtggtgacg cctcaaggga atgcgctgac aattacgttg
     5281 taatctattg acggaatgcc gggtacgtct gagtcgtacc cggcgagatt gatgtttacc
     5341 gcaccggctc cgtcgatgta acttcaccgc tggaaacgac gcccgcattc atggtttgag
     5401 tcacttgctg ctttgcggcg ttcacggcat gcaattgccc gctaaccgta ggtttcagcg
     5461 gggcattggc ctgaatatct ccactggcgg taagctgaat attgccgtcg ccagtcaacg
     5521 gtaattcagg ccatccccat tgttgcaaaa tattcaccgg cacaccgcgt ccattcaggc
     5581 tgatatgggt ctgacgttgt ggcgtttgtg aaacacttgc ggtggcttcc agaatgcctt
     5641 tttcagtaaa tgcactcagt tcgctgatat tcaccgtgct gctgttggcg gtcagcgcca
     5701 gcgacgggcg acgaacatca acacgattga atgtcgcggc ggcggcattc agattcgccg
     5761 agccactcca gacgccccat ttatgatcgg taaccagcgt caggttggca ccgtaaccat
     5821 cgagcgaggt gagctgccac gggaagtcag ggtcgatatc aatgatcaga ttgcggctgg
     5881 cgctaaatct cttcagttgc aggctgttta accaaccggg tgtcgtttcc atccacaact
     5941 gttgccagtt tttcggcaag gtatattcca gcccggcaat tgccgcatca tcaaggatca
     6001 acgttttacc gtcacgcagc cagttccctg acgttctgac cataccccct tcccagcggc
     6061 tggtgaactg gcgcagcgct acgccctgcg gggaaaattc cgcgtttata atcgggtcaa
     6121 ataaatgcag cgaaccataa atgaactcgc tggcgttcat cgacagtttg ccttcctgtg
     6181 tctgccagtc atctttactg aaggtcatgt tgcgcaagct gagatcgagg tcggtcaccg
     6241 cccagtccgg accttgcaga cgagcatcga tgacttccaa gcgaccaatc tgcaacgacg
     6301 ggacagagcg taatggcgca aagaagtcgg ttagcgattt ttcgctttgt agacggatat
     6361 ccgccatgcg cagattttcc acttgccagc tgccgtcggc gttacgctgc gcgtttccgg
     6421 ttaatgtccc gcgggcgatg tcggcaccca ggttagtcag cgtaacgcga tcgttatcaa
     6481 tactgccttc aatcagtaca ttggtggcag gaacatcatt gagcgaaagc gatccggcac
     6541 taaactgaat ctgcgccttc gtacccagca ctttaccggc ttctggtgac cacggaacca
     6601 cgccgccatt tacccgctgc gcgctcagtt tccattcgct attcgggcta ttaaacgcca
     6661 tatcacgcag ttgcagacga tcggctttga acggtagcgg cgcggtctgg tcggtgagat
     6721 tcagcgtccc gttttccagc aggatggtat cgacatggcg tggttcggtc agttggcgac
     6781 tgcttagcgc aatgtcgaca ctttttgcca ccaggggcgc gggctgacca tcacgaccaa
     6841 acgtgacatt ctccagcacg atatgagatg gcgcggaaaa acggtgatcc atcgccccga
     6901 aggccagatg atagtcgcta ttctcggaaa cccatgcgct gatatgttct gctccccagc
     6961 gggtttgcag aagaaaatag aggccagcga tcaccactaa cagagcgatg agaatgtaga
     7021 gaagcagctt cccaataaat ttcatggtct tccatcccgt gaaatgcaca taagggagtt
     7081 atgcacgatt tacgcgcaat cctcaaggcg ggaatggtga aagaggtgag actgcggcgg
     7141 taattatctt accgccgcca gtgaattact gtttttctgg tgggaaaatc agattcagca
     7201 cgatggcagt aataccgccc gcggcgatcc cggaggagag caggtttttc agccattcag
     7261 gggcaaactg caaaatcagc ggctgctgag acacgcccag accaaccgcc agcgacagcg
     7321 cgataatcag aatcgcccga cggttcagcg gctcacgaga aacgatacgc acaccggagg
     7381 cggcgatggt gccaaacatt acaagcgttg cgccgcccag aaccggttct ggaatgtgtt
     7441 gtacaaaacc gctcactgcc gggaacagac ccagcacgat caacatcagc gcgacgacaa
     7501 aaccgacata gcggctggca acaccagtca actggatcac tccgttgttt tgcccgaagc
     7561 aggagttcgg gaaggtgtta aacaccgccg aaacaaacga gttcaggccg tttgccagca
     7621 cgccgccttt caggcgtttc atgtacagcg gaccggacac tggctgttcg gaaacgtcag
     7681 aggtcgccgt gatatcgcca atggtttcca gcgaagtgat cataaagacc agcatcagcg
     7741 gcagcagcag actccattca atgccaagac cgtaatagag cggcgttggc accataatca
     7801 gttcttgcgt catcggttcg ttgctttctg gcaacatgcc cataaaccac gccagcgcat
     7861 atccggccgc catcgcaatt accagtgagg ccacgcgtaa gtaagggtta cgttgacggt
     7921 taagcaggat aattaaggct aaaaccacgc ctgccagcag cagatttttc ggtgcgccga
     7981 aggtgttatc gctcatggct gcgtaaccgc cgccaatgga ggtcagcccc acctgaatta
     8041 gcgacaggcc gataatcatc accacaacgc cagaaaccag cggcgtaata atgcggcgcg
     8101 ccagatgcag aacgcgggag atcaccatct cggtgcaact tgccagcatc aacgtgccga
     8161 acaaagccgc catcatggta ggaacatcag caccaccggt tttcagcgcg gtaccgccca
     8221 taatcagcgg ggcaacaaag ttgaagctgg tgccctgaat agacaacagc ccggagccaa
     8281 ccggacccca ggccttaatt tgaataatcg atgccacacc ggaggcaaac agcgacatac
     8341 taataatgtg ttgcgtgtct tgtgccggta aacccagcgc ctggcagatt aatagcgctg
     8401 gcgtgatcac cgcaacgaac atcgccagca gatgctgaca ggcggcaaac agggtttgag
     8461 gaagcggcgg acgatcttca agacggtaaa tcagttcgct gttttgagtc tgcgcaaccg
     8521 gttgcgcatt ttctgactcg agggtggaaa cagacatcga aaacaatccc gtggtagcaa
     8581 agcgggcatt ttagctgacc gaaagagaaa agcaaacgtt tgccacatcc tcatctaaat
     8641 atttaaatga gataaatgta cttttttata taacttttgg tatttttctg cttaaaatcc
     8701 atgccatttg ttcattaaca gctgagaatt tgacccagga aagcataact cgatacccgt
     8761 catagttcat gaagttgcca ctgcgcgatg aagtatgacg agtatgaaag agtgatgcgg
     8821 atacaaagga gtaactatgt ttcatctcga tactttagca acgcttgttg ccgcaacgct
     8881 gacgttgctg ctcgggcgta agttggtcca ttccgtctcc tttttgaaga aatacaccat
     8941 accggaacct gttgcgggtg gtttgttggt ggcgctggcg ctactagtac tgaaaaaaag
     9001 catgggctgg gaagtcaact ttgatatgtc cctgcgcgat ccgttaatgc tggctttctt
     9061 cgccaccatt ggcctgaacg ccaacattgc cagtttgcgt gccggtgggc gtgtggttgg
     9121 catcttcttg attgtggttg ttggtctgtt ggtgatgcaa aatgccattg gcattggtat
     9181 ggctagcctg ttagggcttg atccgctgat ggggctgttg gccggttcta ttacgctttc
     9241 cggcggtcac ggtacgggcg ctgcgtggag taaattgttc attgaacgtt atggcttcac
     9301 caatgcgacg gaagtggcga tggcctgtgc aacgttcggt ctggtgctgg gcggcttgat
     9361 tggcggtccg gtggcgcgct atctggtgaa acactccacc acgccgaacg gtattccgga
     9421 tgaccaggaa gtcccgacgg cgtttgaaaa gccggatgtg ggacgcatga tcacctcgtt
     9481 ggtgctgatt gaaactatcg cgctgattgc tatctgcctg acggtgggga aaattgttgc
     9541 gcaacttttg gctggcactg cttttgaact gccgaccttc gtctgtgtac tgtttgttgg
     9601 cgtgattctg agcaacggtc tgtcaataat gggcttttac cgcgtctttg agcgtgcggt
     9661 atccgtgctg ggtaacgtaa gcttgtcgtt gttcctggcg atggcgttga tggggctgaa
     9721 actgtgggag ctggcttcgc tggcgctgcc gatgctggcg attctggtgg tacagaccat
     9781 cttcatggcg ttgtatgcca tcttcgttac ctggcgcatg atgggcaaaa actacgatgc
     9841 ggcagtgctg gctgcgggtc actgtggttt tggcctcggt gcaacgccaa cggcaatcgc
     9901 caacatgcag gcgatcactg aacgctttgg cccgtcgcac atggcgtttt tggtggtgcc
     9961 gatggtcggt gcgttcttta tcgatatcgt caatgcgctg gtgattaagc tgtatttaat
    10021 gttgccgatt tttgccggtt aaccgatgaa gtggcggtct tctcaccgcc gccttttacg
    10081 cattcgagta acgttctgtc tccggcatcc agcgttctat caaggctttt gcctgttgtg
    10141 ggtaacgttc gtgaatatgt cgcgccaggc gctgaacttc cgggatcatc gcctgatcgc
    10201 gcagtaaatc cgccacttta aattcagcat tacccgtctg acgcgtgcct aacaattcgc
    10261 cagggccgcg aatctccaga tctttttgcg caatcacaaa accgtcgtta ctgtcgcgca
    10321 gcacttgcag gcgaatttgc gccgttttag aaagcggcgt tttgtagagc agcacgcagt
    10381 gagaagccac cgcgccacga cctacgcgcc cgcgcagctg gtgtaactgc gccagaccca
    10441 gacgctcagg gttttcgata atcatcaggc tggcgttagg cacatcaaca ccgacttcaa
    10501 taacggtagt ggcaaccagc agatgtagct caccttgttt aaacgacgcc atcaccgcct
    10561 gtttctcggc tggtttcatc cgcccgtgta ccaggccaac attcaattct ggtagcgcca
    10621 gtttcaactc ttcccaggta gcttccgccg cctgcgcttc cagtaactct gactcttcaa
    10681 tcaacgtaca aacccagtac gcctgacgac cttcagttat gcaggcgtgg cgcacgcggt
//
LOCUS       DBKEKI010000136        10637 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.137, whole genome shotgun sequence.
ACCESSION   DBKEKI010000136 DBKEKI010000000
VERSION     DBKEKI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.137"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1195)
                     /locus_tag="ACUQPX_003414"
     CDS             complement(<1..1195)
                     /locus_tag="ACUQPX_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HHV9057997.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLERPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAEEQGRGF
                     AVVAGE"
     gene            complement(1241..2902)
                     /gene="tar"
                     /locus_tag="ACUQPX_003415"
     CDS             complement(1241..2902)
                     /gene="tar"
                     /locus_tag="ACUQPX_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HHV9057998.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(3047..3550)
                     /gene="cheW"
                     /locus_tag="ACUQPX_003416"
     CDS             complement(3047..3550)
                     /gene="cheW"
                     /locus_tag="ACUQPX_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HHV9057999.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(3571..5535)
                     /gene="cheA"
                     /locus_tag="ACUQPX_003417"
     CDS             complement(3571..5535)
                     /gene="cheA"
                     /locus_tag="ACUQPX_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HHV9058000.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSTEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFALGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(5540..6466)
                     /gene="motB"
                     /locus_tag="ACUQPX_003418"
     CDS             complement(5540..6466)
                     /gene="motB"
                     /locus_tag="ACUQPX_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HHV9058001.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(6487..7350)
                     /gene="motA"
                     /locus_tag="ACUQPX_003419"
     CDS             complement(6487..7350)
                     /gene="motA"
                     /locus_tag="ACUQPX_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000906339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HHV9058002.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSGETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRVVKNPQ"
     gene            complement(7477..8055)
                     /gene="flhC"
                     /locus_tag="ACUQPX_003420"
     CDS             complement(7477..8055)
                     /gene="flhC"
                     /locus_tag="ACUQPX_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HHV9058003.1"
                     /translation="MSEKSIVQEARDIQLAMELITLCARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(8058..8408)
                     /gene="flhD"
                     /locus_tag="ACUQPX_003421"
                     /pseudo
     CDS             complement(8058..8408)
                     /gene="flhD"
                     /locus_tag="ACUQPX_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
     gene            9188..9616
                     /gene="uspC"
                     /locus_tag="ACUQPX_003422"
     CDS             9188..9616
                     /gene="uspC"
                     /locus_tag="ACUQPX_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HHV9058004.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(9623..>10637)
                     /locus_tag="ACUQPX_003423"
     CDS             complement(9623..>10637)
                     /locus_tag="ACUQPX_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HHV9058005.1"
                     /translation="AHELRKRGVNNRIGFFLHIPFPTPEIFNALPTYDTLLEQLCDYD
                     LLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKAFRTEVYPIGIEPKEIAKQAAG
                     PLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQHHGKIRYTQIA
                     PTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVG
                     LVTPLRDGMNLVAKEYVAAQGPANPGVLVLSQFAGAANELTSALIVNPYDRDEVAAAL
                     DRALTMSLAERISRHAEMLDVIVKSDINHWQECFISDLKQIVPRSAESQQRDKVATFP
                     KLA"
BASE COUNT         2556 a         2790 c         2556 g         2735 t
ORIGIN      
        1 cttcacctgc cactaccgca aaaccacgcc cctgctcttc ggcgcgagcc gcttccactg
       61 ccgcattcag ggccagaata ttggtctgga aagcaattcc gtcgataacg ctgataatgt
      121 cgccaatttt ttgcgagctg gtggcgatct cctgcatggt gtgagtcatg gtactgacct
      181 gaacaccgcc cgcctgcgcc gttgtcgcgg cattttttgc cagttccgac gcctgtcgtg
      241 cgttatcggc gttttgccct accgtggcgg ttaattgctc catactggcg gccgtttgtg
      301 ccagcgatgc cgcctgctgt tcggtacgac ttgagagatc gttattgcct gcgacaatct
      361 ccgcgatccc aatgtgcatc tcatggcttc ccttacgcac atcactcacc gtcccacgca
      421 aagcctgctg catggtcttc aggctggcaa aaatggcggt gatctcatta cgaccatata
      481 ccgcaatcgg acgctccaga ttacccgcag caatgctgtc aaaatggcta ccgataatgg
      541 ccagtggttg aacaatcatc ttgcgcgtcc accacagcgc actgctgatg tagatcgccg
      601 caacaataat catgctgata aacaccagcg ccgaaatctg atagttacgc tggctttgcg
      661 cactggcggc ttccagcaca tggttgatct ccagctgcca ggcctcaaag ttaacgtcaa
      721 acgcattctg tgatccctgc accggcgcag tgaggaaatc cgaaagttga ttactttcaa
      781 gccaggtcgc ctgatgttcg agatcgttgt gccagcgggc aaaacttttc tccgtctctt
      841 tttgcaatgc cctgacgtgc tcgttgcccg cagtcatcgc cataaaactt ttaaacagcg
      901 tggtggattg cgtcagactg gcgcgcgccg tcgtcatcaa cgttttaatg tcatccgccg
      961 gatagctaag cgctgtcaga gtgcctgctt tgttcagcgc ggtactggcc tgtaacatta
     1021 ccgcccgagt ttgcgctaat gccgcacgtt gctgattgct ctgctcgccg tgattcagtc
     1081 gttgcaaatc gtcgcgaaat gcccaaaaag acatgccgtt actgccaatc tgcaagatcc
     1141 cgcagagaat caaaattaaa aacagcgtgg tcgaaattcg aatacgatta aacatcaacg
     1201 ctccccatca ggcggcaatg accgcgttag taaaaactcg tcaaaatgtt tcccagtttg
     1261 gatcttgttc agtaattcgc agtcgtggct gtgccggtgg ttgctcactg gcaggacggg
     1321 atggtgtttg cggtttattg gtgagtgggc tggctgccag acggaacgcg gaaaccgctt
     1381 gcgttaaacg actcgcctgt tcttccagcg cagctgcggc ggcagctgat tcctgcacca
     1441 gcgatgcgtt ctgttgcgtg acgcgatcca tttccgaaac cgccaatgcg acttgatcga
     1501 tgccacggct ctgttcatcc gatgccgatg caatctcgcc cataatgtca gtcacgcgag
     1561 tgacagcatt gacgatattg ttcattgttt ccccggcgct ttcgaccagc accgaaccgg
     1621 tatcaacgcg cgagacggag tcttcaatga gggctttgat ctcttttgcc gcctgggcgc
     1681 tgcgactggc aagattacgc acttcacccg ccaccacggc aaaaccacgg ccctgttcac
     1741 ccgcacgcgc ggcttcaacc gcggcattca gcgcgaggat attagtctgg aaggcaatac
     1801 cgtcgataac gctgataatg tcggcaattt tcttcgaact atcggcgatc tcatgcatcg
     1861 ttttcactac gccatccacc actttgccgc cgtgctgggc ggtgtcggag gcactttgcg
     1921 ccagttgcga ggcctggcgg gcgttatcgg cgttttgctt cactgtcgcg gtgagctgct
     1981 ccatgctggc ggcagtttct tccagcgcgg atgcctgctg ttcagtacgg gaggaaagat
     2041 cggtgttgcc cgccgcaatt tcacgggtac cggcatagat ggcatctgaa ccttcgcgga
     2101 catgagtgac ggtgtcagtc aaagagcgtt gcatatgtga aacgctctgc gccaggtcac
     2161 ccatttcact gcgcccgtca atggtcaggg tattcgccag gttaccaccg gcgatttcgc
     2221 gaatgtgagc aataattttt gccagcggag taagcaacat acggcgaatg ccgtaccacg
     2281 ccaccagcag aatcaatacc accaccagcg cgataaccgc cagttgccac tgggcaaatc
     2341 ggtaatcatc tgcgttgtca gtgacgatat cgcgatacag tttttcactg ctgagggcgt
     2401 actgagcaaa cgcttcgccc attgcatttt gcattccctg ggttggctga gcgaaataag
     2461 ctccagtatt gccataatca agataatcaa tcagttcagt taacgctgtg taatagtttt
     2521 tatatttttc atcaaaatta cggctggtag cgaccatttc aggtaacggt gccatgcttt
     2581 tgaatttttt ataatgcgtc gcggcctggg ccaatgtttt cctggcgcta tcgagcaatt
     2641 caactttggc gttactttgt tgattggagg aatccatcat catccgcacc gctgaacgac
     2701 tcaggttaat gcgcgtttgc agcattaaat cccaggttga cgtcagctcg ccctgctgtt
     2761 cccgtaattg attggaaacc acaaagctct tctggctatg gtgaagggaa gaaaaaaaca
     2821 aactgccgga aataagctgt aacagtgcga atacccccag caccattacc aacagcgtga
     2881 ctacgcggat acggttaatc ataaggcacc ttcctgaaaa caagttgatc tcgttatcgg
     2941 caagaagggg ggaaacttta ttgctgatgc cacccgccgc gaaattgaaa taaaaaaccc
     3001 gatgcgcaga tcatcgggtt catttcaatt gaggaaatcg ggagaattac gccacttctg
     3061 acgccgcgct atctaacagc gccatctctt cgctgttcag cagtttttcg atgttcacca
     3121 gaatcaacat acggtcgccc agtgcgccca gtccagtgag atattctgtt gaaagcgtca
     3181 cggcaaactc tggtgccgga cgaatttgct ccgccgtcaa tgaaagcacg tctgaaacgc
     3241 cgtcaaccac gatgccgacc acccgctgtc cgagattcag gacgataact accgtgttgt
     3301 cgttatagtc cacatccacc tggctgaact taattcgtaa gtcaacaatc ggcacaataa
     3361 cgccgcgcag attcgtgacg cctttgataa acgctggcgt gttcgcaatc cgtgttacct
     3421 gatcgtagcc acggatctcc tgcactttca ggatatcaat accgtactct tcatcaccaa
     3481 gggtaaatac cagaaattcc tggcctgacg gctcgctggc cagctttgtt acattcgtca
     3541 taccggtcat attgttacct ttttactcat tcaggcggcg gtgttcgcca tacgttgttc
     3601 gcggtttatc gcctgcaagg cggagacatc aacaatcagt gccacgctgc cgtcgccaag
     3661 aatggtcgca gcagaaatgc cggggacttt gcgatagtta ctttcaaggt ttttaaccac
     3721 aacctggtgt tgaccaatta attgatccac cagcaaggcg tagcggcgac cgccactttg
     3781 taagatcacc acaattccct gggtggcttc ggttttcgcg cccgcgacgt tgaacacttt
     3841 ccacagttcg acgatgggca gatattcacc ccgcacttcc agcacccgct cgccgccggc
     3901 cagtggatgg agatcggctt cacggggttg cagtgattcc ataacagcat tcagcggcag
     3961 aatgaaaact tcatccgcaa cgcgtacgga catgccgtcg aggatggcca gcgtcagcgg
     4021 cagtaaaatg cggatcgtag tgccagtacc ctgcttcgac tggatttcga catgaccgcc
     4081 catctcctgg atattacgtt taacgacgtc catgccgacg ccgcgcccgg agacgtcggt
     4141 gacctgctct gccgtggaga agcctagtgc aaatatcagc attgcaactt cgtcgtcgct
     4201 catgttttcg ctgacagtca aaccttgcga ggccgctttt gccagaattc gctcacggtt
     4261 tagccccgcc ccatcgtcgg tcacttcaat gcaaatgttg ccgccctgat gttcggtaga
     4321 cagaattaaa tttccgacgc tgtttttacc tgcggcgagc cgtttttctg gcagttcaat
     4381 accgtgatcg aggctattgc gtaccaggtg ggtcagcggg tcgataatgc gttctatcag
     4441 gctcttgtcg agctcggtgg aactgcccac cagcgtcagt tctacctgct tgccgagttt
     4501 tcccgccaga tcacgcacca gccggggata gcgactaaag acatattcca tcggcatcat
     4561 gcgaatcgac atcactgatt cctgcaaatc acgggcgtta cgttgtaact gccccatgct
     4621 ggtaatcaaa tcaccatgat taaccgggtc cagttcgctg gaacgctggg caagcatgga
     4681 ctgggtgata accagctcgc cgacgaggtt aattaattga tcaacctttt ctaccgctac
     4741 acggatgctg gtggattcac tgctgcgcgt cgttttttcc cgctccacgc ggccggttgg
     4801 cgcttgttcg gctgccagtt taagcacagg tggggtagct atttttggcg aaacttctac
     4861 tgtttcaaag gtaatctgat cggcttcaat cacaaaacag agtaccgctg tgatgtcatc
     4921 ttcggcgata tcgcccggta atattgccga gagagaatcc gcccctttca ccacgtcatt
     4981 taacgttgtc agatgcccca gctcttcttc cagcaggtcg acttcgccgg ccttcaggcg
     5041 cgaaaggata attcgtcgcg gcaactgact gcgactttgc tcatcttgcg gttcactttt
     5101 ggcaaccaca cttaatcggg tcactgcgga tggcgtttcg cctttcgctt ctaatgccag
     5161 ttgacgcaag gcctggcaga tataatcgta gctggcggca tccggctctt gcgactgttt
     5221 ataagcgtcg agctgttctt gcatgatgtc cttcgtttcc aaaaacagat tgataatgtc
     5281 ggtgttgagt tgcatctcac ctcgtctggc ttcatcgagc aggttttcca tcagatgcgt
     5341 ggtttcctgc aaaacgctga agccaaaagt tcctgcccct cctttgatcg agtgggcagc
     5401 ccgaaagatg gcattcaatt gttcggcatc tggcgcttcc ggctgcaaaa ccagcagatg
     5461 ctgctccata tcagccaaca gttcgtccgc ttcatcaaaa aatgtctgat aaaaatcgct
     5521 tatatcgatg ctcacgctgt cacctcggtt cggctgatgg cattgtggga acactgacct
     5581 gtggtgcaac ctcaggtttt tccagggcgc ttactggctc attctggctt tcggcgtttt
     5641 catgcaaaat ggcctgttcg gcttgtttgt tcagtaccag caggctgatg cgacggttga
     5701 cggcatcatc aggtccgcga tcgcttaagc gcatcgttgc cgccatgccg acgacacgta
     5761 acactttgcc gctatccaac cctccgacca tcagttcgcg gcgggatgca ttggcccgat
     5821 cggcagaaag ctcccagttg ctgtaacctt tctcaccgct ggcgtaggga aaatcatcgg
     5881 tatgacctga aaggctaata cggttgggaa taccgttcag cacaggcgcg atggcgcgca
     5941 gaatgtcgcg catatagggt tcgacatcgg cactgccggt tctaaacatc gggcgattct
     6001 ggctatcgat gatctgaata cgtagacctt cctggaccag atcgattttg agatggagac
     6061 gtaacgcccg cagtttcgga tcggactcta tcaactggtc gagatcaccc cgcaatttcc
     6121 gcaatcgact ttgctccatg cgttttttca gctcttcgat gttcggctgc ttattcactt
     6181 ccccctggct ttgggtgtaa tcatcaccac cgccgggaat tgggctttca ctattggaaa
     6241 tgcgatcgcc gcccgtaacc gctgtcgcca gtggagtccg gaagtactcc gcaatctgaa
     6301 tcaactcttt tgggctggag atggagatca gccacatcac cagaaaaaag gccatcatcg
     6361 cagtcataaa gtcggcataa gcaatcttcc acgatccatg tgctgccccg tggcttttgg
     6421 ctttgcgtcg tttgacgaca ataatcggat gcgcttgatt cttcatgctt cctcggttgt
     6481 cgtctgttat tgcggatttt tcaccacacg cacatgctct tccagttcaa taaacgacgg
     6541 acgttcgctg gaatagagcg ttttgcgacc aaactcaacg gcgataggcg gtgcgtaacc
     6601 gttcagatta gaaagcagag tgactttgac gcactgcatc attttgctgg tttcgccgct
     6661 tttctgacgt aaaacactcg ctagtgggga aataaatccg taagccaata aaataccgag
     6721 gaaagtcccc accatcgcat gtgcgataag cgcccccagc tcggcggcag gacgatcggc
     6781 tgaacctaac gcgtgaacgc cccccattac agccgcgaca ataccaaacg ccggaagtga
     6841 gtccccgacc agcgccagac tgttcgccgg gacttctgcc tcgctttcgt gcgtctcaat
     6901 ctcttcatcc atcagggctt cgatttcgaa ggtgttcatg tgaccgctga taatcaggcg
     6961 cagataatcg acgataaaat caagcatgac gctatccgcg aggatgcgtg ggtagctggc
     7021 gaagatctcg ctctcacggg gattttcaat atcacgttcc agcgaaaaca tccccatctg
     7081 ccgcgatttc gccatcaacc gataaagcag agccagcaga tccatataca ttgctttggt
     7141 gtatttggag cgacgaaaca gcaacggcag cgctttcagc gtgcctttaa tcgctttgcc
     7201 attattgccg acgataaacg acccaatccc tgcaccggca ataatcacca gttcagcggg
     7261 ttgatagagt gctccaaggc ttccaccggt catcaaataa ccgccgaaaa ctgtaccgag
     7321 aacaaccagg taacctaata agataagcac gacatcatcc ttccactgtt gaccatgaca
     7381 ggatgttcag tcgtcaggcg ttaacgcgcg attggggcaa aaaaaagcag cggtacgtcg
     7441 ttaccgctgc tggaatgttg cgccacaccg tatcagttaa acagcctgta ctctctgttc
     7501 atccagcagt tgtgggataa tatcggcagg attctgggaa agtttacgtc tttttactgc
     7561 ccgggatggc ggttgacata agctgcaggc aaagctgcca acaggctggt gagcgtgggt
     7621 aataaaattg ccgccgcagc agttgcagct ggaaagttgc agtaatccac tttcaacaaa
     7681 ccgcaccaat gtccaggcac gggttaatgc cagcagtggt ccttcttctg cttgtgggca
     7741 ctgttcaagg tataaacggt aggctttgat caccgcatcg acgccattac acaaaccggt
     7801 tttcagtaaa aactgccatg cattacagaa catcgaagca tgaacgtttt gttcccaggt
     7861 cataaaccag tcggttgaga atggcagcat gcctttcggc ggtgggcttc cgcgcagttc
     7921 tttataaagt tttatcaggc gtccgcgact taactgtgtt tcgctttcca gcatctgcaa
     7981 acgagcgcac agggtgatca attccattgc cagctgaata tcccgcgctt cctgaacaat
     8041 gcttttttca ctcatgatca ggcccttttc ttgcgcagcg cttcttcagg ctgattaaca
     8101 tcattcagca agcgtgttga gagcatgatg ccggtatgaa tttgctggag atcgtcaacg
     8161 cgggaatctt gcgtcaactg agtaatcgtc tggtggctgt caaaacggaa gtgacaaacc
     8221 agctgattgg tttctgccag cttaaccatt tgcggaagag tcagtgccgc taacgttgtc
     8281 gccatttctt catttatgcc gagacgaaac atagcggacg ctttgtccta aacaatcaga
     8341 cgctgtgcaa gtagtaaata tgacaagttg atgtcataaa tgtgtttcag caactcggag
     8401 gtatgcatta ttcccaccca gaataaccaa ctttattttt atgcggtctc accgcacccc
     8461 gtgatgtcgc cgggaagctc cggtaaaaaa taattagcat tagaatagtt gcgataagct
     8521 gcaataagca gagccacctt tttggtttaa taagtcctta caaatagaag ggggtcttta
     8581 cacttatcta agatttttcc taaatcgacg caactgtact cgtcactaca cgcacataca
     8641 acggaggggg gctgcgattt tcaataatgt gtgatgcaga tcacacaaaa cactcaatta
     8701 cttaacataa atgtataagt catacttttg ttttgggtgt atttcaatct gttaaaaagt
     8761 ttttcgctac gctagcaagc aaaaatgaaa caggaataat cgaaatggga tgttgcgcac
     8821 ggttaaaata actcaccgta aataatcatc tgctataaat aatcactttc atgcaatacc
     8881 agataagcta tttttaaaca gacacttacc gcacaacaaa ctaattaaat acaaagaaag
     8941 gcaacaatca acttaatgtt aataaaatat ttttcatata ttaaccactt gtttaatgcg
     9001 gggaatcagt acattatttt cttataatta cagttgaaat catatgatca ccgagagtga
     9061 ttgattaatt gtctttacta ataatcagac taatatttac ctgtttgact gagttgggat
     9121 tgcgtcgttt ctccattagg agtaaagctt taatgtcacc tgaagttcac agaataagga
     9181 acaggttatg agctatagca atattcttgt cgctgttgcg gttacaccgg aaagtcagca
     9241 actgctggca aaagcggtat ctatcgccag gccagtaaag ggacacatca gtttaattac
     9301 tctcgcttcc gacccggaaa tgtacaatca attagccgcg ccgatgcttg aagatttacg
     9361 cagcgtgatg caagaagaaa cgcaaagctt tcttgataag ttaattcagg atgcggggta
     9421 tcccgttgac aagacgttta ttgcctacgg tgagttaagc gaacatattc tggaagtatg
     9481 tcacaaacat catttcaatt tggtgatttg cggtaatcac aatcacagtt tcttttcgcg
     9541 agcatcctgc tcggcgaaaa gagtgattgc ctcaagtgag gtcgatgtgc tgttagttcc
     9601 acttacggga gattaaccgc tcctacgcaa gctttggaaa ggtagcaact ttatcgcgct
     9661 gctggctttc cgcgcttcgc ggaactatct gctttaggtc gctaatgaag cactcctgcc
     9721 agtggttaat atcgcttttc acgataacgt ccagcatttc tgcatgacgg gaaatacgtt
     9781 ccgccagcga catagtcaat gcacgatcca gcgcagctgc aacttcgtca cgatcgtagg
     9841 ggttaacaat taacgccgac gttaactcgt ttgccgctcc cgcaaattgc gaaagaacaa
     9901 gaacgcccgg gttggctggg ccctgagcag caacatactc ttttgctacc aggttcatcc
     9961 cgtcacgcag tggcgtcact aagcccacgt cagagtagcg gaatattttc atcagtaatt
    10021 tacggtcaaa atgctgattc aaataataaa gcggcgtcca gcctaattgc ccgtatttac
    10081 cattaattcg tccagcttca ttttcgagct gatgacgaat atcctggtag gcttgcacat
    10141 caccacgcga cgttggtgca atctgggtat aacgaatttt accatgatgc tgcggatatt
    10201 tttccagcaa tgcctcatag gcgagaaaac gctccggcaa acctttggaa taatccagcc
    10261 gttcgacaga aaagatattt tgtacgtttt tcaattccgc tttaagttgc gccagttttg
    10321 gcggcagtgg cccggcagcc tgtttggcta tttctttcgg ttcaatgccg atcgggtaga
    10381 cttctgttcg aaatgctttt ccccaggctg tatggctttt tgcgctacgt gtcgtgacgc
    10441 gggtcaggtt agaaaggcaa tccaggaacg ccagacgatc gttttctgtc tggaaaccca
    10501 gcaaatcata atcacaaagc tgttcaagca aggtgtcata tgtcggcagc gcgttgaaaa
    10561 tttccggtgt cgggaaagga atatgcaaga aaaaaccaat gcgattattc accccacgtt
    10621 tgcgtaattc atgcgca
//
LOCUS       DBKEKI010000137        10592 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.138, whole genome shotgun sequence.
ACCESSION   DBKEKI010000137 DBKEKI010000000
VERSION     DBKEKI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.138"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            218..1138
                     /gene="cbpA"
                     /locus_tag="ACUQPX_003424"
     CDS             218..1138
                     /gene="cbpA"
                     /locus_tag="ACUQPX_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HHV9058006.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPSSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1138..1443
                     /gene="cbpM"
                     /locus_tag="ACUQPX_003425"
     CDS             1138..1443
                     /gene="cbpM"
                     /locus_tag="ACUQPX_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HHV9058007.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(1536..2135)
                     /gene="torD"
                     /locus_tag="ACUQPX_003426"
     CDS             complement(1536..2135)
                     /gene="torD"
                     /locus_tag="ACUQPX_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HHV9058008.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIATLTVRDDARLELAADFCGLFLMTDKQVALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFAALCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(2132..4672)
                     /gene="torA"
                     /locus_tag="ACUQPX_003427"
     CDS             complement(2132..4672)
                     /gene="torA"
                     /locus_tag="ACUQPX_003427"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HHV9058009.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPDGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            complement(4672..5844)
                     /gene="torC"
                     /locus_tag="ACUQPX_003428"
     CDS             complement(4672..5844)
                     /gene="torC"
                     /locus_tag="ACUQPX_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HHV9058010.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            5974..6666
                     /gene="torR"
                     /locus_tag="ACUQPX_003429"
     CDS             5974..6666
                     /gene="torR"
                     /locus_tag="ACUQPX_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HHV9058011.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(6639..7667)
                     /gene="torT"
                     /locus_tag="ACUQPX_003430"
     CDS             complement(6639..7667)
                     /gene="torT"
                     /locus_tag="ACUQPX_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HHV9058012.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGNPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLECHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            7750..10461
                     /gene="torS"
                     /locus_tag="ACUQPX_003431"
                     /pseudo
     CDS             7750..10461
                     /gene="torS"
                     /locus_tag="ACUQPX_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
BASE COUNT         2506 a         2877 c         2756 g         2453 t
ORIGIN      
        1 aacttaaagt gcctggttca actatcaaaa atcgctcacc ctttttcacc tgtttaaaat
       61 atgttcagca acccatcttg atggcgacct cctctccgcg atgatttcaa taacatattc
      121 tgtgttggca tatgaaattt tgaggattac cctacactta taggagttac cttacagggg
      181 ttccttcaat ttgtgttgat ttacgcgaga taacgctatg gaattaaagg attattacgc
      241 catcatgggc gtgaaaccga cggacgatct caagacaatc aagaccgcct atcgtcgact
      301 ggcccgtaaa taccatcctg atgtcagcaa agaaccggat gccgaagccc gcttcaaaga
      361 ggtcgctgaa gcctgggaag tgttaagtga tgaacaacgt cgcgctgagt atgatcagat
      421 gtggcaacat cgcaacgatc cgcaatttaa ccgtcagttc catcatggcg acggtcagag
      481 ttttaatgcc gaagattttg acgatatctt ctcgtcaatt ttcggtcagc atgcccgcca
      541 gagccgtcaa cgccccgccg cacgcggcca cgatattgaa atcgaagtgg cggtattcct
      601 cgaagaaacg cttactgagc ataagcgtac catcagctat aacctgccgg tttataacgc
      661 ctttggcatg atcgaacagg aaatcccgaa aacgctgaat gtgaagatcc cggcaggcgt
      721 cggcaatggt caacgcatcc gcctgaaagg ccaggggacg ccgggcgaaa acggcggtcc
      781 aaatggcgat ttgtggctgg tgattcatat tgcgccacat ccgctgttcg atattgtcgg
      841 ccaggatctg gaaattgtgg tgccggttag cccgtgggaa gcggcgctgg gtactaaagt
      901 caccgttcca acactgaaag aaagcatttt gctgactatc ccgccaagca gccaggccgg
      961 gcaacgattg cgcgttaaag gcaaaggtct ggtgagcaaa aaacagaccg gcgatctgta
     1021 tgcggtactg aaaatcgtga tgccgccgaa accggatgaa aacactgccg cgctgtggca
     1081 gcaactggca gacgcccagt cgtcttttga tccacgtaaa gattggggga aagcataatg
     1141 gctaatgtta cggtgacttt tactattacc gaattttgcc tgcataccgg catctctgaa
     1201 gaggagttga atgaaattgt cggtttgggg gtggttgaac cgcgtgagat ccaggaaaca
     1261 acctgggtat ttgacgacca tgccgccatt gtggtgcaac gcgcggtacg cctgcgtcat
     1321 gaactggctc tggactggcc ggggatcgcg gtggcgctga cgttaatgga tgatattgcg
     1381 cacctgaagc aggaaaaccg cctgctacgc cagcggcttt cccggtttgt agctcatccg
     1441 tgatgtgtgt ttgccggatg tgatgacgca tccggcaact gcgccataat gcctgatgcg
     1501 acgctaaacg cgtcttttca ggcgcataaa cgacgttatc tgttttggtg gtcgcactcc
     1561 actaacacca gcaataactg gcttagtgcc gcgtaaaaac caaagctgtc atactgtcga
     1621 caaagcgcag caaactctgg taaccattgc cgcagcgccg tcagtgtttt ttgccgcaaa
     1681 ctgtcgattc ttcgcgcagg aacggtcccc tctcccagcg aaaaatgcag atgactcaac
     1741 aactcgagat agatcgccag atgatctgcc ggttcgttga aattgccact ggtttccatc
     1801 cctgcctcaa ctaacaagcg tttaatctct tgctcgtcct gtttgtaggc cgatgcatac
     1861 ggcagcgcca cttgtttgtc ggtcatcaga aacaggccgc aaaagtcagc ggccagttcc
     1921 agacgggcat cgtcacgaac tgtcagcgtg gcaatactgt tttccagatc attcaccgcc
     1981 gcagtgagcg gcggttcgct tttcagcaac gaaaaccatt cagccatctg cgcactggcg
     2041 atttgcgtca gttgttcatc gtccagttca cgggagaaca actgcgccag ccaggcgtaa
     2101 acacaggcaa tctgttgtgc tgtcagcgtg gtcatgattt cacctgcgac gcgggaacat
     2161 attcgcactg cgccaccatc tccacggggc cgttaaacgc cgtcacctgc tccactgttc
     2221 cattgtactt ctcaatttcc accagcgtag tgtgcgcact ggtcgcctgc gccagctgcg
     2281 aagtaccgat gtcgatggtc agcacgttgg ggttaccgta tttgcacagc gcaccaggct
     2341 cgccgccttt atctggatcg taccatgccc cttcgtgaat tcgcgccacg ccgggtgcat
     2401 agcggtcaga aactactgct cctgccaaca cctgaccgcg agcgttaaag acgcgtacca
     2461 catcaccgtt acgaataccg cgcgcgctgg catcctgcgg gttaatgaac actggctctt
     2521 tacccgctac cgtatattgc tgacgcagcg tttccgactc acataactgc gagtgaagtc
     2581 ggaaatccgg atgcacagat tgcagatgca acggatactt ctgcgagccg ggcccaccgt
     2641 gggagcgttc gattttctca aaccacatcg gatgcccctg acaatcgtcg tagttcatat
     2701 cggcgatggt tttcgagtag atctcaatca ggccactcgg cgtgcccagc ggttcgagat
     2761 ccggatcttc gcggaatgcc tggtggcgaa caaacatctg cggatggtca aactcgacgt
     2821 actctttgtt attccagaag tcatcaaacg ctggcagatg aacgccgcgt cctttgcctt
     2881 gttgtacacc ttcctgccag atgcgtttca gccagcccat ttcgtccagc ccttcggtaa
     2941 aggcttcttc acgattaaag cgacggcaca gctcgcggaa aatatcaaag tcgttgcgcg
     3001 cctcgaactg cggcggcacc acctgtttca tggcgataat gccacggttg gagtggttgc
     3061 cgtactggtc gagatcgtta cgctcaaact gcgtggtcgc aggcagcacg atatcggcaa
     3121 agcggcaggt tgaggtccac tggttatcta tggcgataac cgtttccagc ttgcgccagc
     3181 cttcaataat gcggttgatc tgctgatggc gatggaatgg gttagttccg gcaaaaatac
     3241 acattttcag cggcggcagt tttaccgatt taccgttcca gttgatcact ttccccggtt
     3301 cgaggatcgc atcgataaaa cgggcaatcg gaatagtgct gctgtagcct ttgtagtcac
     3361 tgttgtcgtg aacaggcgga atcgacgtag agccggagaa accactcaga ataacgcctt
     3421 tacgccccgg cgtgcctgcg ccgttgtagt gccagccaaa accaccatct ggcaggccaa
     3481 tttgccccag catcgccgcc agaaccacaa tcatccacgc ccactgttca ccgtgctgca
     3541 tacgctgcac gcaccagcca gcaataattt gcgttctgtt cgccgccatc tgccgcgcca
     3601 gcccacgaat ggtttcggca tcaatgccgg tcagtttttc agcccatgcg gcatctttcg
     3661 gctgaccgtc tttctcaccc agcagatacg gcaggaactg ctcaaaaccc acacagtagt
     3721 tagcgaggaa gtttttgtcg tacaggtttt cactgtacag cgtatgtgcc agcgccagtt
     3781 gcagcggcac gtcagtttgc gggttaaccg caatgtgctt cacatgctcg cgccccagat
     3841 actcatgggt ggatgtgaca accggatcga tgctgatgac ctcaacttca ccggcggcga
     3901 ctttcgcttt tagctgctcg taatattcat aaacatcgtg atccgggcac caccagttcg
     3961 cttgctggtt tttcagcaaa tcggagcccc acagcacaat ggttttgctg ttctgcaata
     4021 ccagcggcca ggaggtttgc tgttcataca cttccattga accgactacg cgcggcagga
     4081 tcacctgcgc agcaccggta gagtaatctc cgcccgtacc aacgctatta ccatgcaagg
     4141 caatagcttt cgccagcatc cccgaagcgt tatggaacat ccccgtcgat tgccaaccac
     4201 tggcggtcag caaggcactc ggcccgtgag ttttctgtac gcgttccagt tcttcataga
     4261 acatgtcgag ggcttcatcc cagctcacgc gcacaaaacg gttatcaccg cgctgggagg
     4321 tgtcgctcag atggcgctta cgcagccagt ccacgcgtac catcggataa cgaatacgtg
     4381 ccgcgttgtg tacgtgatcc ggcaatccgg cgatcatttt cgacggatat ttatccagtt
     4441 cgaacggttt tgccgccaca aagcgaccat ccttcaccgt cgcgcggata gccccccagt
     4501 gcgacccggt aagaatgccc tctttcgaga tgacagcctc agtcgccgct tgcgccgcag
     4561 ttgcacggcg cggcgttaac aatgacggcc ccagcatccc ggcgacggtt aagccgccga
     4621 gttgtgccag aaaacgccga cgtgatgcct gaaagagatc gttattgttc attatttttc
     4681 ttccttctta tcgccgtgag ccttacctgc ggtgtcagac gcattcattt gcagatattt
     4741 caacaaagtg cgttcttcac gtttatcgag actggtaaag ccaatcatgc cgttgagcgt
     4801 gccgatccaa ccgttagcgt caaagtgggc gatttccggt gcgccgtggc actggttaca
     4861 ggtgccgttg tacaacgaat ccgcataggc ccagatcggt ttgatatcgt tcaccatgtc
     4921 gcctttcttc atccacgcgg tggcctgcaa cttgctccac tcggtattgg tgtcggcaac
     4981 ggtggttttc tccagcgttt tcacctgctg ctgcacatca ccacgaatcg aggcaacaaa
     5041 gatgcgttta cctgggaatt gggtgagtac acgctgacgt ccggcgcttt ccgtccagcc
     5101 ggtaatttca atttgcagcc agtcgccgtc acgtttaagg actttcactt ccgaagcagg
     5161 cagcagagaa ccagaggctt ctttatcgcc tttcgccgca taaattggct taatatcaat
     5221 agagtacagc gtgtcaccac tgtcattagc actggcgcgc agctcatcga actgcttacg
     5281 gaagccgcta ctcatatccg gtaactggtg ggcaatacct ttatgacagt cgatgcagga
     5341 ttgattatct ttcgctgcca ccttcatctg acgtgccgct tcaggatgct gcctggcatg
     5401 atccatcgca tcgtagttat ggcaggagcg acaggttgcc gagttgtttt ctttcattcg
     5461 cgcccattca cgctcggcaa gttccgcgcg tttggcttcg aatttttcag gtgtatcaat
     5521 ggagtgggca ataaaggtct ggtaaatgtc gttgctcgct tccagtttgc gcttcaccat
     5581 gcctggaata tccggcggga tatgacagtc atggcattca gctcgcacgc cggaggcgtt
     5641 ctggaaatgc accgactgtt tatattcttc atacaccggt tgcatactgt ggcaactgac
     5701 acaaaattcg gttgtgctgg tgactttgat cccaacgtgt ggcaatacaa tcagcgcaat
     5761 gccaatcaca atcccaattg cgaccagcgc cagtaccgac caacgagcac tgggtcggcg
     5821 tagcgcgttc cagagtttcc gcataataac ccctgtagaa ttatggttta gtgaagcgat
     5881 cttaatgagc aaatatgaac agcggcactg gtcaggatga acggcttacg gcagaatatg
     5941 aacagatatg aacagaatga gtaaaaccct ctgatgccac atcacattgt tattgttgaa
     6001 gatgagccgg ttacccaggc gcgattacaa tcctacttca ctcaggaggg gtataccgtt
     6061 tccgttacgg cgagcggtgc cgggctacgg gaaattatgc agaatcagcc ggtagattta
     6121 attctgctgg atatcaactt acccgatgaa aatggcctga tgttaacccg cgccctgcga
     6181 gaacgctcaa cggtggggat tattctggtt accggacgca gcgatcggat tgaccgtatt
     6241 gttgggctgg aaatgggcgc agacgattac gtcaccaaac cgctggaact gcgcgaactg
     6301 gtagtacggg tgaaaaatct gctctggcga atcgacctcg cgcgacaagc tcaaccgcac
     6361 actcaggact actgctatcg ctttgcgggt tattgcctga atgtgtcgcg ccatacgctg
     6421 gagcgggatg gcgagccgat taaactgacc cgcgcagagt atgaaatgtt ggtggcattt
     6481 gtgactaatc cgggcgaaat tctcagccgt gagcgtctgc tgcgtatgct ttctgcgcgt
     6541 cgggtggaaa accctgacct gcgcaccgtc gatgtgttaa ttcgtcgttt acgtcataaa
     6601 ctcagcgcgg atttactggt gacgcaacat ggtgaaggtt atttcttagc cgctgatgtg
     6661 tgctgataaa aatagaccgg acgaaatccc cccggtgaca gcgagcggcg gatatgttct
     6721 cggtcggcat ttttcggcgt cagaactaaa atcggtgggc tgacattatc agacaccgat
     6781 tgcccctgta attgcctgat ggcctgctca actgccagtt ccccctgcca gaccatttga
     6841 tcgctggcag ccataatcac tcttccccgc ttcagcccgc gatacacctg atgtgaaaga
     6901 taaaacgaca ccacggtaag cggcgttttc aggttacgcc cttcacccat tgccgcctct
     6961 gccgcaatgg ccgttccggc aacgacgtcg atttctgggt ggcattccag catctcctgc
     7021 aacaggttgc gctggatttc aatatcgtta tcaccaagcg caatatcaac aatacgcacc
     7081 gggcttccgg caatggctgc gcgaaaaccc tcgaccatct ccttactgcc cccggcgtta
     7141 tcgggtccgg gcatcagcag cacgttcagt gggttaccgt gcgcccattg caccaaatat
     7201 cgcccaggtt gatagcccat ctgaaaccag ggtacaccaa cgcggctttt cacctgggga
     7261 tcatcaagag catttaccag ttcgatcacc ggcagatttg ctacctgctt ttgcaggtcg
     7321 ggaaatgacg tcgtgctact accgagcaaa atggcctctg cgccccactg tttacactgg
     7381 tcgatttgtg cttgctgggt agccaactgg ctgtagccgc ctgcctccag cacttttaaa
     7441 tccacaccgt agcggcgagc agcctcctgc ataccatagt tcaacgataa ccagtatgaa
     7501 tctttcaggc tgggataaag cgcgcacagt ttccatacgc gtttggcttt aagcggcata
     7561 gaggcttgca ccgtgaaatg ctgcgcatca tgccagcgca acaggttatc agccgaaaat
     7621 gccgacaaca tgaaaaggga aagaagtaaa aatagcagta cgcgcatgat agcctcatca
     7681 ataataaggc tttatgctag atgcattcca ctttgcgact caaccttttt caccttaagt
     7741 gcaccgaccg tgaatttaac cctgacccga agactctgga tgggctttgc cctgatggcg
     7801 ctgttaaccc tgaccagtac cctggtggga tggtacaacc tgcgctttat cagccaggtg
     7861 gaaaaagaca atactcaggc attgattcct accatgaata tggcgcgcca gttgagcgaa
     7921 gccagcgcct gggaactttt cgccgcgcag aacctgacca gtgccgataa cgaaaagatg
     7981 tggcaggcgc aggggcgaat gctcaccgca caaagcctga agattaatgc gttgctgcaa
     8041 gcgttacggg aacaaggttt tgataccacc gctattgaac aacaggagca ggagatctcc
     8101 cgttcattac gtcagcaagg ggaactggtg gggcggcgtc tgcaactacg ccagcaacaa
     8161 cggcaactca gtcagcagat agctgccgcc gatgagatcg cacgcctggc gcaaggtcag
     8221 gcgaataatg cgacaacttc cgctggagcg acccaggccg ggatttacga tttgatcgaa
     8281 caagatcagc gtcaggctgc tgaaagtgca ctcgatcggc tgattgatat cgatcttgag
     8341 tatgttaacc agatgaatga actgcgcctt agcgctctgc gggtgcagca aatggtgatg
     8401 aatctggggc tggagcagct ccagaaaaat gcaccaacgc tggaaaagca gctcaataat
     8461 gcggtgaaaa ttctgcaacg tcggcaaata cgcattgaag atccgggagt tcgtgcgcag
     8521 gtcgcaacaa cgttaactac cgttagccaa tatagcgatc tgttggcgct gttccagcag
     8581 gacagtgaaa tcagcaatca cctgcaaact ctcgcccaaa ataatatcgc ccagttcgcg
     8641 cagtttagta gcgaagtcag tcagctggtc gacaccattg agctgcgcaa tcagcatgga
     8701 ctggcacatc tggaaaaagc cagtgcacgc gggcaataca gcctgttatt gctggggatg
     8761 gtttcacttt gcgcactgat tctgatcctc tggcgcgtgg tttatcgctc agtcacgcgt
     8821 ccacttgccg aacaaacgca ggcgctgcaa cggctgctgg acggtgatat tgactcccct
     8881 ttcccggaaa ccgctggcgt acgggagctg gataccatcg ggcggctgat ggatgcgttt
     8941 cgcagcagtg ttcatgcact gaatcgccac cgcgaacagc tggcggcgca ggtcaaagcg
     9001 cgtacagctg aattgcagga actggtgata gaacaccgac aggcacgggc ggaagcagaa
     9061 aaagccagct aggcaaaatc ggcgtttctg gcggcgatga gccatgagat ccgcacaccg
     9121 ctgtacggta ttctcggcac tgctcaactg ctggcagata accccgcact taacgcccag
     9181 cgtgatgatt tgcgggcgat taccgactct ggcgaatcgt tgctgaccat cctcaacgat
     9241 attctcgatt attcggctat cgaagcaggt ggcaaaaatg tttcagtcag cgatgagccc
     9301 tttgaaccgc gcccgctgct ggaaagtacc ctgcaattaa tgagcggacg ggtgaaaggt
     9361 cgcccgattc gcctggcaac ggcaattgcc gatgatgtac cgagcgcgtt aatgggcgat
     9421 ccacgacgta ttcgtcaggt cattaccaac ctattgagca atgccctgcg ttttactgac
     9481 gaagggcaca ttatcctgcg tagtcgcact gatggcgagc aatggctggt cgaagtggaa
     9541 gacagcggct gcggtattga tcccgcgaaa ctggcagaaa tcttccagcc atttgtgcag
     9601 gtaagcggca aacgcggcgg caccgggctg gggctgacta tcagtagccg tctggcccag
     9661 gcgatgggcg gcgaactgag cgccaccagc acgccggagg ttggaagctg tttttgttta
     9721 cgcttgccgt tacgtgttgc cactgcaccc gtgccaaaaa cagccaatca ggcggtgcgt
     9781 cttgacggtt tacgtttgct gttaattgaa gataacccgc taacccagcg aattaccgtt
     9841 gagatgctga acaccagtgg tgcgcaggtt gttgctattg gcaatgccgc gcaggcttta
     9901 gagacactgc aaaatagcga accgtttgct gccgcactgg tggattttga tctgccggat
     9961 atcgacggca ttacccttgc ccggcaactg gcacagcaat atccgtcgct ggttttgatt
    10021 ggctttagcg cccatgtcat tgacgaaacg ctgcgccagc gtaccagttc actatttcgc
    10081 gggattatcc ctaaaccggt gccgcgtgaa gtgctcggtc agttactggc gcactatctc
    10141 caactgcaag ccaataacga tctaccactg gatgtctcgc aactcaatga agacgctcag
    10201 ttaatgggga cggagaagat ccacgaatgg ctggcattat ttaaacaaca tgccctgccg
    10261 cttctcgatg aaatcgacat tgcccgcgcc agccaggaca gcgagaaaat aaagcgtgcc
    10321 gcgcatcagc taaaaagcag ttgctcgagt ctgggaatgc gtagcgccag tcagctatgc
    10381 gcacaactgg agcagcagcc attatctgcc ccccttccac acgaagaaat tacacgcagt
    10441 gttgccgctc tggaagcatg attgaataag aaagacctga acgcgatttg aatcaatatc
    10501 agccagttaa gaataactag aatagtggca tttggtatgc aacttaacgg actggcaaaa
    10561 tcatggcaga gaaaaaaaga acccgctggc ag
//
LOCUS       DBKEKI010000138        10473 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.139, whole genome shotgun sequence.
ACCESSION   DBKEKI010000138 DBKEKI010000000
VERSION     DBKEKI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.139"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            22..186
                     /locus_tag="ACUQPX_003432"
                     /pseudo
     CDS             22..186
                     /locus_tag="ACUQPX_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
     gene            204..1163
                     /gene="lsrB"
                     /locus_tag="ACUQPX_003433"
     CDS             204..1163
                     /gene="lsrB"
                     /locus_tag="ACUQPX_003433"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HHV9058013.1"
                     /translation="MNVQAAERIAFIPKLVGVGFFTSGGNGAQQAGKELSVDVTYDGP
                     TEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPALKRAMQRGVRVLTWDSDTKPEC
                     RSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYSSPTVTDQNQWVKEAKAKIAKE
                     HPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAIIAPDANALPAAAQAAENLKNDK
                     VAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISVYVADALLKKGSMKTGDKLDIQ
                     GVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKENIGKYDF"
     gene            1190..2065
                     /gene="lsrF"
                     /locus_tag="ACUQPX_003434"
     CDS             1190..2065
                     /gene="lsrF"
                     /locus_tag="ACUQPX_003434"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HHV9058014.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            2089..2379
                     /gene="lsrG"
                     /locus_tag="ACUQPX_003435"
     CDS             2089..2379
                     /gene="lsrG"
                     /locus_tag="ACUQPX_003435"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HHV9058015.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            2418..3176
                     /gene="tam"
                     /locus_tag="ACUQPX_003436"
     CDS             2418..3176
                     /gene="tam"
                     /locus_tag="ACUQPX_003436"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HHV9058016.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(3180..4094)
                     /locus_tag="ACUQPX_003437"
     CDS             complement(3180..4094)
                     /locus_tag="ACUQPX_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HHV9058017.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(4301..5752)
                     /gene="uxaB"
                     /locus_tag="ACUQPX_003438"
     CDS             complement(4301..5752)
                     /gene="uxaB"
                     /locus_tag="ACUQPX_003438"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HHV9058018.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(5979..7397)
                     /locus_tag="ACUQPX_003439"
     CDS             complement(5979..7397)
                     /locus_tag="ACUQPX_003439"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HHV9058019.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(7536..7895)
                     /locus_tag="ACUQPX_003440"
     CDS             complement(7536..7895)
                     /locus_tag="ACUQPX_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HHV9058020.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(7895..8821)
                     /gene="glsB"
                     /locus_tag="ACUQPX_003441"
     CDS             complement(7895..8821)
                     /gene="glsB"
                     /locus_tag="ACUQPX_003441"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HHV9058021.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(8885..9850)
                     /locus_tag="ACUQPX_003442"
                     /pseudo
     CDS             complement(8885..9850)
                     /locus_tag="ACUQPX_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            9924..>10473
                     /gene="yneK"
                     /locus_tag="ACUQPX_003443"
     CDS             9924..>10473
                     /gene="yneK"
                     /locus_tag="ACUQPX_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HHV9058022.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYHVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPD"
BASE COUNT         2811 a         2636 c         2533 g         2493 t
ORIGIN      
        1 taaaaaacgg gaggccgcat aatgcgtatt cgctacggtt gggaactggc tcttgccgca
       61 ctgctcgtta ttgagattgt cgcatttggt gcaattaacc cgcgaatgtt agatctcaat
      121 atgttgctgt tcagcaccag tgactttatc tgcattggca ttgtcgccct accgctgacg
      181 atcttggcat tgccgcaatc tctatgaatg tgcaggccgc agagcgtatt gcatttattc
      241 ccaaactggt tggcgtggga ttttttacca gcggtggcaa cggcgcacaa caagcgggta
      301 aagagctgag cgttgatgtg acctacgacg ggccgacaga acccagtgtt tctggtcagg
      361 tacagttgat taataacttc gtcaatcaag gttataacgc cattatcgtt tctgcggttt
      421 cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca acgtggtgtg agagtgctga
      481 cctgggactc tgatactaaa ccggagtgcc gctcttacta cattaatcag ggaacgcccg
      541 cccagttggg aggtatgttg gtggatatgg cggcgcgtca ggtgaataaa gacaaagcca
      601 aagtcgcgtt tttctactca agccccaccg ttacggacca aaaccagtgg gtgaaagaag
      661 cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat tgtcactacg cagtttggct
      721 ataacgatgc cactaaatcg ttacaaaccg cagaaggaat attaaaagcg tatagcgatc
      781 tcgacgccat tatcgccccc gatgccaacg ccctgcccgc tgccgcacaa gccgcagaaa
      841 acctgaaaaa tgacaaagta gcgattgtcg gattcagtac gccaaatgtg atgcgcccgt
      901 atgtagagcg cggcacggtg aaagaatttg gactgtggga tgtggttcag caaggcaaaa
      961 tttcagtgta tgtcgcggat gcattattga aaaaaggatc aatgaaaacg ggcgacaagc
     1021 tggatatcca gggcgtaggt caggttgaag tctcgccaaa cagcgttcag ggctatgact
     1081 acgaagcgga tggtaatggc atcgtactgt taccggagcg cgtgatattc aacaaagaga
     1141 atatcggcaa atacgatttc tgatgtgcat aacttaaccg gagtaagtta tggcagattt
     1201 agacgatatt aaagatggta aagattttcg taccgatcaa ccgcaacaaa atatcccttt
     1261 taccctgaaa ggttgcggtg cgctggattg gggaatgcag tcacgcttat cgtggatatt
     1321 taatccgaaa acgggtaaaa ctgtgatgct ggcctttgac catggttatt ttcaggggcc
     1381 gactaccgga cttgaacgca ttgatataaa tatcgccccg ctgtttgaac atgccgatgt
     1441 attaatgtgt acgcgcggca ttttgcgcag cgtagttccc ccggcgacca ataagccggt
     1501 ggtactgcgg gcgtcaggtg cgaactctat tctggcggaa ttaagtaatg aagccgtggc
     1561 gttatcgatg gatgatgccg tgcgcctgaa cagttgcgcg gtggcggcgc aggtttatat
     1621 cggcagcgaa tatgaacatc agtcgatcaa aaatattatt cagctggtcg atgccggaat
     1681 gaaagtggga atgccgacca tggccgtgac tggcgtgggc aaagatatgg tgcgcgatca
     1741 gcgttatttc tcgctcgcga ctcgaatcgc cgctgaaatg ggggcgcaaa ttatcaaaac
     1801 ctattatgtc gaaaaaggtt ttgaacggat tgttgccgga tgtccggtac ccattgttat
     1861 tgctggcggt aaaaaattac cggagcgcga ggcgctggaa atgtgctggc aggctatcga
     1921 tcagggcgct tctggtgtgg atatggggcg taatattttc cagtctgacc atccggtggc
     1981 gatgatgaaa gccgtacagg cggtggttca ccataacgaa acggctgatc gggcatatga
     2041 actctatctg agtgaaaaac agtaactgcg gatttaagga gaagaattat gcacgtcaca
     2101 ctggttgaaa ttaacgttca tgaagacaag gttgacgagt ttatcgaagt ttttcgccag
     2161 aaccacctgg gctctgtaca ggaagaaggt aatttgcgct tcgatgtctt acaggacccg
     2221 gaagtgaatt cgcgctttta tatttacgaa gcctataaag atgaagacac agtggcattc
     2281 cataaaacca cgcctcacta caaaacctgt gtcgcgaaac tggaatcttt aatgaccggg
     2341 ccgcgtaaaa aacgtctgtt caatgggttg atgccgtgag gctacaattg gggtaatgcg
     2401 ctgacgggag taaaaaaatg tctgactgga acccctcttt atatctgcac ttttccgctg
     2461 aacgatcgcg tccggcggtg gagctgcttg ccagagtgcc gctggagaat gtcgaatatg
     2521 ttgccgatct tggctgtggt ccaggtaaca gcaccgccct gctgcatcaa cgttggcctg
     2581 cggccaggat aacaggcatc gactcgtctc cggcgatgat tgctgaagcg cgcagtgctt
     2641 tgccagactg ccagttcgtg gaagcagaca tccgcaactg gcaaccggaa caggcactcg
     2701 atctgatttt tgctaatgcc tcactgcaat ggctgcccga ccactacgaa ttgtttcctc
     2761 acttggtttc tttacttaat ccgcagggcg tgctggcagt acagatgcca gataactggc
     2821 tggagccgac ccatgtgctc atgcgcgaag ttgcctggga acaaaactac ccggatcgcg
     2881 ggcgtgagcc gttggctggc gttcatgctt actacgatat tttgagcgaa gccggatgtg
     2941 aggtcgatat ctggcgaacc acttactatc accagatgcc gtcgcaccag gcgattatcg
     3001 attgggtgac cgccactgga ttacgtccgt ggttacagga tctgaccgag agcgaacagc
     3061 agctttttct tacgcgctac catcagatgc tggaagagca gtatccactg caagagaacg
     3121 gacagatact gctggcattt ccgcgtctgt ttattgttgc ccgccgtacg gagtaattat
     3181 cacgtcagct ggtaatgacg atcgggaaga atttttgctg gaatttctgc ttcatcgttc
     3241 atctgtaaca ggtcaatttc aatagcgttg cagatggcat ccagcggtaa atcattgttt
     3301 tcagtaccga acggatcttc cagttcttcc gccagacaat ccagcgaaat aaaagtgtag
     3361 gaaatcagca cagagataaa aggcgtcatg taatgcaggt ccacgaccag cgcgaacggc
     3421 agcatgatac aaaacagata aacggtacga tgcaaaatca gggtgtaggc aaagggaatt
     3481 ggcgtgtagg caatgcgctc acagcctgcc agaaccgctg aaatatcatt aagccgatcg
     3541 ttgaggctaa taaacaggat atctgaaagc tgtccattgc ggcgctgaac cgccaaccat
     3601 tctcccatta ttaacaagat acggttagct ggagagttcg aagccagtac acgctgaaga
     3661 tcttcagtct tgagataatg agccagcact tccgcctgtg gctgtttgcg taatgtcatg
     3721 cgtaaacagt gggcgaaggc gatttgcagc ctggcaaact cccttacact tgccgaatcc
     3781 ggcaatgtcg tttttacctc tcgcagtaac gaccgtgagg caatcatcaa ctgtccccaa
     3841 agttttcgcg cttcaacgta acgggcgtac ccggcattat tacgaaaacc aagaaaaatg
     3901 gcgatggcga caccgagaat gctgaacggt gcgagggtga atttgatgcc cagatgcgtg
     3961 taccagggca gcatgaatat aacagcaata gaaaagagaa aattgagtag taagcgcgag
     4021 gatatcttgg ataatactga gccgtgccag acaaaaatac ggcgcagcca gtgttgttgt
     4081 ggacgaacaa tcatggttat cttcaggcgt ggaaaagtcg ccctattaaa cgtgattacg
     4141 atcacattct caagacgcta cttacaaatt acctacaagc ttacaacatt aacaggcgct
     4201 gcatgtgaca cctgttaatg ataaaggcat ataggatgtt gtaactaata tagtgaaata
     4261 agaaacccgg tcgaaaccgg gttcagaagt aacggtgtta ttagcacaac ggacgtacag
     4321 cttcgcgcat ccctttttca agaatcgcat ccaggtcatt agcaacctgc tcgaccagac
     4381 caggcacttg cgtcaggtcc tgctcccagt ggtctttttc tgccaacaca atcgctacca
     4441 gttcctgggt accgataaca cggtcacgat gctggctcca gagttgctga taacgttcca
     4501 gccagtgtgc atcgtcctgt accggatatg tttctccgtt acgctcacca cgatagaacg
     4561 caatcaatgc cgctaatgcg aaagtaaggc gcgccggaag tgtgccgttt gccttctgcc
     4621 ctgccagcag ctgcggcagg atgcgggtgc ggaacttggt cataccgttg agcgcgatag
     4681 acagcagctg atgcttaatg tacgggttac ggaaacgccc ggtgactgca ctggcgaaag
     4741 attccagttc atcacgaggc aaatccagta ccggaataat ttcttcgtag atggcttttt
     4801 caacgaatgc gcaaatttca gcatcgttca tcgcttcacc tacggtatcc agccctgcct
     4861 ggaacgccac cggcaccagc gcggtgtgcg caccgttgag gattgccact ttgcgttctt
     4921 tatacggttt aatatcgtca acaatcagca cgttgagcgg atatttgtcc aggcgcagct
     4981 cagtcgctaa ggatttcggc ccctgaatca caaacaggta aaagtgttca gcggtgtcga
     5041 ggaaaccatc gtgataaccc agttcttctt ccagtttagc cacttcatcg cgcggataac
     5101 cggtaacgat acggtccacc agcgtagaac agaagctgtt agcctgatcc agccattgaa
     5161 taaatgcttc tggcagtgcc cactcttgcg catagcgcag caccagttca cgcaacgcgt
     5221 cgccattgta gtcaatcaac tcacacggaa tgatgatcca acctttatcc agcgcaccgt
     5281 tgaaatggct aaagcgttcg aacagcagac gggtcagttt tgccggatag cttaccgctg
     5341 gcgcgtcatc gaatttatcg cccgcatggt agctgatacc cgcttcggtg gtgttagaga
     5401 aaacaaagcg catttccggg ttgtgcgcca gtttcaggaa ttcatcgtat tcactgtaga
     5461 cgctgatttc acgattaacc gagcgaatca gacgcgcgtc gctgaccgct tcccccttct
     5521 cattcaggcc acggataatg gtggtgtaca gaccatcctg cgtgctcagt gacggcggga
     5581 atgaagtttc aatcggacga acaacgacca cgccagaatt cagatcggtg tgctcattca
     5641 ggagatcgat ttgccagtca acaaaggcgc gcaggaagtt accttcacca aactgaatga
     5701 tacgttctgg atactgtgca ccgggaaaat cgcgacgatt tagtgttttc acaatgggtt
     5761 cccttctgat tagtcataca acctgtttga attggtgcga caggttagca aactttaata
     5821 cgccgacccc ctgttttgat caactcctga tgattaatga gcagttttat gagaaaagtg
     5881 tggcgcggat catggtttaa tcgaggaaat acgccttttc ctggatcata aagtggtaga
     5941 acacattgca ttcaaatcgc gcgaaatgaa taaagatgtc agacaacttc ctcaccgtaa
     6001 cgcatagtgc tggtacggtt gcgcccatct ttcttcgagc gatacagaca cgtatcggct
     6061 tcgaccatca atttattaaa atcatcggtc agcgttcggt acgatgcgcg accactaccg
     6121 acgccaatac ttaccgtgag ataaagcgtt ttttgttgcc aggtgaatgg ttgcagttca
     6181 acgcctttac ggattttttc cgccattagc agaccatcga caggattcac cgacggcact
     6241 gcaacagcaa attcttcgcc gcccatgcgc gccaccagcc ccttatcacc gacaatcttc
     6301 tgaatatgcc gggcaaacac gcttaacact ttatcgccac attcatgtcc atagttatcg
     6361 ttaatgcttt tgaaatagtc gatatcaagc agcatgaccg tcagatgttg tgtctgtttc
     6421 agcgatggac ttttcagcgc ctcataaaga ccggaccgtg agtaaacctg agtcagaaaa
     6481 tcaaagtcgg ctcgcagcgc aacttgcttc attagcaaat tgatcgctgc cacgctaaaa
     6541 gaaaccataa ttgggcatat cgccatcgtg gcaataccga gacgtgcgga gaacatttcc
     6601 ggaatggaga acggcgaacc gaccgagata tcaatcaccg aatttgccac cagtacgatt
     6661 tccaccgcac cggtgacaaa ggtcaacaga catgttacct gcggcgtata gcgcactgca
     6721 caccagatta atgcaggcag cgggaacgcc agactccccg ctccgccaat gactaccgag
     6781 gcaataaccg acacaataag cgcgatggcg ggcatcatct gttctgcttt aaagcgcggc
     6841 agcactccag gaatagccaa cgtcagcata caaggcacga tcaacacgcc cgttgagaat
     6901 tgctcactga accagtccgc aagcaaaggc cagaaatcca gactatcaat actgaccgaa
     6961 ccaatcgccc cgacaatagc gcataataat gcggcaatca gacagtaatt aaatagccgt
     7021 aaggcactta ctggctcata cttatttttc cccagacgct tatcccgcac gaccagtaag
     7081 gcgacggtaa caataaacat catattggag aaattgataa ccagtgaaac cagtccccat
     7141 tcggtggtta tggcatcata aaccagcatc gccacataac tgatcgcata gtaatgcagt
     7201 cgattaagcc agacatagcg ggcaaacacc cccgccatta cgccattcag cggccagaaa
     7261 agagaaagtt cgtgaaccag ccgtaactct gcaccaataa aatagaacag cgtggttagc
     7321 gcaaaaatag cgatgctatt acgtagcaga tgaccttcct ggaacaaacg gaaagttgaa
     7381 atggggtgta catgcattaa ttatctgtcc atacaattgc gggtggcaat tatctcaacg
     7441 taataagtaa attctcataa aggatagcat gttcgctaac agcggcggca aataaaggta
     7501 ccctgggaat gctattttat cgttttccgc aattctcatg gctgattcat ctgtacaacc
     7561 agccagtgat agattaccgc cacgatataa cgttgttgtt cttcgcttaa cgaaacaccc
     7621 tgcggaatgt tatgccactt agccagacaa ccgcgacagc aggtcgcggt ggcgtgctga
     7681 gcgataaaca ccggatgccc gcgcatgggg gtttgcttac cgtcattagc cggtaatgcc
     7741 ggagcaagac gtttagcgac aaaatccgcc gcatgttgtt cgatgactgg cgcgcctttc
     7801 tccaggcaat actgacgctc tttcatgccc agacgaaagc gagagcgaaa ttttgaacgg
     7861 gataaacgcg cgaagagtgg atcgagagac tgcattaata aaccgaacgc cctaactgtt
     7921 tcgtcaattg ttcaaggacg gcgatacccg caagcgagtt acctgcatca tccagttccg
     7981 gactccagac tgcgatggcc atttcatgcg gaacaatcgc cacaataccg ccaccaacgc
     8041 cagatttcgc cggtagtccc acccgccagg caaactcccc cgcgttctgg tacataccac
     8101 tggtcgccat cagcgcgtta atttgccgcg cctgcattgg cgtcaccaca ggttcatcaa
     8161 tatgaatagc tttcccctga ttagccagaa agacaaacgt ccgggccagc tctacacagc
     8221 tcattttcag agcgcagtaa tgaaagtagt tttgcagaac ggttgtcacg tcatgatgga
     8281 aattgccaaa cgacttcatc agccaggcga tagccgcatt tcgcgcggaa tgttcaaatt
     8341 cggaacgcgc taccaccgta tcgtatgaaa tatcagacac accgcttaag ccgcgcacga
     8401 cttccagcat acgttgccgt ggtgcgctta gtcgcccttg caacatatcg cagaccacca
     8461 gcgcaccggc attaatgaac ggattacgcg gaataccctg ttccatttcc agttgcacta
     8521 aagaattgaa cggtgatcca gacggatctt tgccgacgcg ttgccagatt tcctcttcgg
     8581 agtaatgacg catggcgaca acgagactca gcactttgga aatagactga atggaaaaac
     8641 gttcttgcgc gtctccggcc tgaaaaagct gtccgtcaac ggtacagata gcaatcccca
     8701 atcgggaacc gtctactgta gccagcgccg gaatataatc cgcgacttta ccctgaccaa
     8761 tgagcggccg cacttgccgc aagatgtttt ctaaaattgc attatccatg gcgactgcca
     8821 ctttctactc ttggaccgca ggtctgaaaa gacctgcgag tatatcagag ctgaatatgt
     8881 cgcgtcagat ccggtctttc cacactgtct ggatattaca gaattcgtgt aagccgaaat
     8941 gggaaagctc acgaccaaag ccactctttt tcacgccacc aaaggccact cgcgcgtcgc
     9001 tggcacaata accattgata aacaccccac cgcattccag acgtgccgcc atctgtctgg
     9061 cctgtgtttc gtcggtggta aaaatggtcg ctgaaaggcc gaactcacta tcattagcca
     9121 gttccagcgc atgttctgca tctttcgcaa tggtgattgc agcaacgggg ccaaacattt
     9181 cttcccgaaa cgcggtcatt tctggggtaa cattcgccag aaccgttggc ggatagtagt
     9241 tacctgcccc agccatcttt tccccgccca gtaacaaacg cgcaccctga gccagggttt
     9301 tctccacctg atgatgcagc tcatcacgta aatcaaaacg agccattggt ccgagagcgt
     9361 tctcttcatc acggggatcg cccattttca aggctgccgc agctgacaca aaacgttcgg
     9421 taaatgccga agcaattccc tcttcgataa taaagcgttt tgccgctgca caaacctgtc
     9481 cggtattctg ataacgtccg gctaccgccg ctttaaccgc cagttccaga tcggcatcgt
     9541 taagcacaat aaatggatcc gaaccgccca gttccagtac gcattttttc agtgccgctc
     9601 cagcctgtgc gccaatagcc gctccagcac gaacacttcc ggtcaccgtg acagcagcaa
     9661 tgcgcgagtc tttaatcatc tgactgacac cgtcgttgtc ggcattcagc cagccatata
     9721 cgccttgcgg gattcccgca tctttaaaca cttgggcaat gagctgagca cagcccatca
     9781 cattcggcgc atgtttaagt aagtagccgt tacctgcaag aatgatggga acagcgccac
     9841 gcatcacctg cctttgatag ttcttgcagc tcaaatgccc tatatagtga tgttccgatg
     9901 acttatgact atatggggca aatatggtta cgccagtaag catcagcaat tacatatctc
     9961 ttcctgatga ttttcccgct cgtaatattg cacctcaagt aaaagaagtt ttaaaggatt
    10021 ttattgatgc actcagtacc ataatatgtg atgaagagtg gcgcacgtct ttaaacacca
    10081 actcagccac aaaaaagata tttaacaacc ttgataactt atcatatatt cagaggactt
    10141 cttttcgggg taacgacaca ctatacaatg aaaaggttca gtttaaactc acttatcacg
    10201 tcaagaatgg aagacacaaa gaaaatattg aatttcaggt agtaataaat ttaagcccta
    10261 tttatttaga taattttcgc catgatggag aaattaatat tttttgcgct cccaacccaa
    10321 agcctgtcac tatggggcgc gtatttcaga ccggcgtcga gcgtgtgctc tttctgttta
    10381 tgaatgattt tattgaacaa ttcccaatga tcaaccttgg cgcccccatc aaaagagcgc
    10441 atacaccaca tattgaaccc ctgcctccgg atc
//
LOCUS       DBKEKI010000139        10404 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.140, whole genome shotgun sequence.
ACCESSION   DBKEKI010000139 DBKEKI010000000
VERSION     DBKEKI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.140"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(38..364)
                     /locus_tag="ACUQPX_003444"
                     /pseudo
     CDS             complement(38..364)
                     /locus_tag="ACUQPX_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(374..922)
                     /locus_tag="ACUQPX_003445"
     CDS             complement(374..922)
                     /locus_tag="ACUQPX_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HHV9058023.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(922..1629)
                     /locus_tag="ACUQPX_003446"
     CDS             complement(922..1629)
                     /locus_tag="ACUQPX_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HHV9058024.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAISAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIANGLGQLPATIVYSWAGSMLTGCTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1644..2321)
                     /gene="ydjY"
                     /locus_tag="ACUQPX_003447"
     CDS             complement(1644..2321)
                     /gene="ydjY"
                     /locus_tag="ACUQPX_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HHV9058025.1"
                     /translation="MSQHYSVSWEKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2326..3036)
                     /locus_tag="ACUQPX_003448"
     CDS             complement(2326..3036)
                     /locus_tag="ACUQPX_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HHV9058026.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3203..4009)
                     /gene="xthA"
                     /locus_tag="ACUQPX_003449"
     CDS             complement(3203..4009)
                     /gene="xthA"
                     /locus_tag="ACUQPX_003449"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HHV9058027.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4455..5675
                     /gene="astC"
                     /locus_tag="ACUQPX_003450"
                     /pseudo
     CDS             4455..5675
                     /gene="astC"
                     /locus_tag="ACUQPX_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            5672..6706
                     /gene="astA"
                     /locus_tag="ACUQPX_003451"
     CDS             5672..6706
                     /gene="astA"
                     /locus_tag="ACUQPX_003451"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HHV9058028.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            6703..8181
                     /gene="astD"
                     /locus_tag="ACUQPX_003452"
     CDS             6703..8181
                     /gene="astD"
                     /locus_tag="ACUQPX_003452"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HHV9058029.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            8178..9521
                     /gene="astB"
                     /locus_tag="ACUQPX_003453"
     CDS             8178..9521
                     /gene="astB"
                     /locus_tag="ACUQPX_003453"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HHV9058030.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            9514..>10404
                     /gene="astE"
                     /locus_tag="ACUQPX_003454"
     CDS             9514..>10404
                     /gene="astE"
                     /locus_tag="ACUQPX_003454"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HHV9058031.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHD"
BASE COUNT         2393 a         2658 c         2916 g         2437 t
ORIGIN      
        1 aaaaagagcc agtcagttgc ttaactgact ggcattactc ttttaaggtg cggaagtttt
       61 cgccgttcac ccagagcaga tccaccgagc cgcccgtttt acgaccggca gcagcttcgg
      121 tctgaatgcg cttcaccgcg tctgcggcat ccgcgagacg aacaatcttc aggtttatag
      181 cgtaatgggt tttcatctcg ccgctaaccc agtcgagata gcggttaatt gcggtatcgc
      241 cgccccaggc gttaaaccag acggtttgcc ctttggcctc atttttaatt tcctgccagt
      301 cactggcatg tgttgccaaa gaaaacagcg ataacaatcc cagcaaccac ccacaatggc
      361 gcatcttccc cccttaagaa cgtttttccg gaatacggca cagcccctga gcgggaatgt
      421 ccatggcaga attaaaacga gccgacaggt tttgcaggcc gatcaatgcc gtcagttcgg
      481 tgagcgcctg agcgtcaaaa tgcgcagcca gtcgggtacg cagggcatca tcgactgttg
      541 gcggtgtgac gcttgcggct tcggcgtact ccagcgccag ccgttcttca tcgctaaaga
      601 gcgggctttg ccgccagtca gccactgcca gcagtttatc ggtgctgccg gtacgctcgg
      661 cgactttcat actggtgata tccacgcaaa actcacacag gcacatttgc gcaatgcgcg
      721 cgctgacaag cgatcgcact accggatcga gcggtgagcg tttgcgctcc agccacccaa
      781 caaacattga caccaaataa aagataaacg ggatccgccc ccaccagcga atcgggctta
      841 gcacctcgcc gtaatggcga cgatgcagcc acgcctgcgg acgtaaaatg aaaggaattt
      901 tgctaagcgg cggtaaaccc atcagtcatt cctcttctgg cgctcaagcc atattttctt
      961 cgccataaaa atcaccacgg tcagggcaaa cagaataaac agtccggtga caaaccagaa
     1021 agtacagcct gttaacatgc tgcccgccca ggaataaaca atagtcgccg gtaattgacc
     1081 aagcccgttg gcgataaaaa acgagcgaaa acgtattgaa gtcagaccgg cagcatagct
     1141 gattggatcg aaagggacaa aaggcaataa ccgacagacc aggatggtgt gtttgccgta
     1201 gcgagtgaaa aagccgtcca tactgtcaag cacggttttg ccggttaatt tttccaccac
     1261 ttcgcggccc atcactctgg cgataaaaaa gcacagcgcc gcgccggcca tcgaactggt
     1321 ccacgacagc aagccgcccc agaacgcgcc aaacagcgac gcattggcaa aggtgatcaa
     1381 aaacgcaggt agcggtgcag aaatggcctg taaaatcatc aacaagaacg agacaactgc
     1441 cgccagtgcg ccgtaagact gaataaagcg ttctataccc tgttggtcca cggcggcaaa
     1501 cgccgcaacg ctgcgattga taaactcatg gacgccggga agcagcgccc atgcgagcag
     1561 catagcgaac aacaggatga tgagggttat acggtagtac cagattttac gcgattgtat
     1621 cttcatcatc ctttatcctg gctttattcg gtgattttaa aggtaaccgt tgccagcgtg
     1681 ttatccgccg ggagaactga ggcattgcct ttgaatttca cttcgccgcg tttttccacc
     1741 gcaccataag tgtatgttgc attgctgacg atgccgaccg ggcagctatc cagacacacc
     1801 aggcaacccg ttttcttctc ttctgctgcc gttaaattac cgccaaagcg catgtccagt
     1861 ttcttgccat tactgtcaac aatcacttca tcgaaggaat acgcttttgc cgccccttgc
     1921 cagttgaccg aaatatccag tttgctgcct gtgacatgag tcgtttcttt attatccatc
     1981 gtcatgtttt cgcccggcgt accacctgcc tctttcaggg cttcataaaa tgctttcggg
     2041 gtcgcataag ccataaacag cgatttatgt ccgttggagc catctttaaa cacaataccg
     2101 tgacgagtgt cgtcggtgag gaaacgacca ttaatttgca ccagcatggt taccgtatgg
     2161 ttattggcat cgacgcgcag cggttggctg ttcgaaagtc cgtcgtattc cacttttgcc
     2221 gccgcattct ctttttgatc gcagccgcta agcccggcaa cagccagtaa gcagagtgca
     2281 gccagtcctt tttcccatga cactgaataa tgttgcgaca tcgttctatc cttcattttt
     2341 tgggtgagta agtgggctgc ggcgcgaagc agacagatcc acatgtttgt gtcgggcgta
     2401 gagttttgcg agctggatga gaataaacag cgccaggccc gccagacaga gttgtaaaat
     2461 aaagcgcagc gtaatgcctt cattggcgag atcgcttgcc atcacggtat aaataacaat
     2521 accgggcagg gtcgtgagtg ccgaaataag ggtataaggc cagaaggcga tggtggttaa
     2581 tccgtaagcg taattttgaa tattgtaagg aaacaacggg attaagcggg tcagaataag
     2641 aaaatcaata ccgttacgcg caatgccttt ttcaatggcc tggaaggtat ggctatggcc
     2701 cacgtatttc agcagtaaat cacgtcccag ccagcgcgcc agcaggaatg agcacgagga
     2761 ggccagcgtt gcggcaatta atgagagtag tgtccctaag agcgggccaa aaactattcc
     2821 tccagcgatc accagtatgc ttcctggtaa cagaaagagg gtggcattga tgaataacag
     2881 aatatagagg ctataaccga acaatccgct ctggcgaatg agtgtctgta agtggggtaa
     2941 atcggtgagc agatcgaata aaccgaaagc gtggatagcg taaatgacca gcgcaaaaat
     3001 aagacaggca aacagaaagt tacgctcagc gttcatcgtg gagtttccta tatcaaactt
     3061 gataatagag aagtggtcag acaactaaaa acgcagtgac ggtataacaa aggacggcag
     3121 gcaacaaatc attactgaca gtttgttgat ttttctcaag gttaattctc ctgacccagt
     3181 ttgagccagg agagctgcta aattagcggc ggaaggtcgc ccagacgggg gcgtgatcgg
     3241 acggtttttc catgctgcga atttcatagt cgatgccggt ttctacgcaa cattctgcca
     3301 gcggttggct ggcgagcagc aggtcgatgc gcagaccacg gttatcgtca aaaccttttg
     3361 agcggtaatc aaaccatgag aaacgatctg ctgtttgcgg attagcatgg cggaaggtat
     3421 cgaccacccc ccagctcatc agcctgtcca tccattcgcg ctcttccggc aggaaagagc
     3481 atttaccggt acgcagccag cgcttacggt tttcttcgcc aatgccgata tccagatctg
     3541 tagggctgat attcatatcg cccataatca gtaccggatt atcacgtttg agttcggttt
     3601 ccaggtagtt ttgcagattc tgataaaact gcgcttttgc cgggaatttt atcggatggt
     3661 cgcggctttc accctgcggg aagtaaccgt tgatcacggt gacattaccc agcagtgagg
     3721 ggatttccgc cataataatc cgccgctgcg cctcttcgtc gtcaccggga aagccgcgac
     3781 gcacggcaat cggcgtctct ttggtcagca gcgccacgcc ataatggcct ttctgcccgt
     3841 gataaaacac gttgtagccg agcttcgcca cctcttcgag cggaaacata tcgtcatgaa
     3901 cttttgtctc ctgcaggcca atcacatccg gttggtgctt ttcgacgatg gcttcaagct
     3961 ggtgaggtct ggcgcgcagg ccgttgatat taaaagagac aaatttcata gtcgctgcca
     4021 tttattcaga taaagagtgc gtggatggta gcagaatttc gcgttgctta ccgcctgttg
     4081 ttaagtgatt tgatcgatgg ttgttaatga tgtcaacgat ggcgcaaaaa atgcccgctt
     4141 ttggtgcgcg ctgcgtcaga atggcgcagt aatttccagt aaatttcgtc aatgatcaca
     4201 attccagaat tatattttac ttctgcataa cattgcgttt tttattcttt ttattcaccc
     4261 acatcgcaaa cgtattcact ttatatgcac tttaaatgca tatggtttgg ttataactcc
     4321 ttgatttcca gttagcctcc gccgtttatg cacttttatc actggctggc acgaaccctg
     4381 caatctacat ttacagcgca aacattactt attgttaaca tacaaataac gaattattta
     4441 ctgtagaggt cgctatgtct cagccaatta cgcgtgaaaa ctttgatgaa tggatgatac
     4501 ctgtttacgc tccggcaccc tttataccgg tacgtggcga aggttcgcgc ttgtgggatc
     4561 agcaggggaa agagtatatc gacttcgcgg gtggcattgc ggtgaacgcg ctgggccatg
     4621 cgcatccgga actgcgtgaa gcgctgaacg aacaggcgag taagttctgg cataccggca
     4681 acggttacac caacgagccg gtactgcgac tggcgaaaaa attgatcgac gccacgtttg
     4741 ccgatcgcgt cttcttttgt aactccggtg cggaagccaa cgaagcggcg ctaaaactgg
     4801 cgcgtaaatt cgctcacgac cgctacggca gccataagag tggcatcgtg gcgttcaaaa
     4861 atgcgtttca tggtcgcacg ctgtttactg tcagtgcggg tgggcagcca gcctattcac
     4921 aggattttgc gccactgccg gcggatattc gtcatgctgc atataacgat attaactctg
     4981 ccagcgcgct gattgacgac gccacctgtg cggtgattgt cgagcccatc tagggcgaag
     5041 gcggtgtggt gccagccagc aacgcgtttt tacaaggtct gcgtgaattg tgtgaccgcc
     5101 acaatgcctt gctgattttt gatgaggtac aaaccggcgt cgggcgcact ggtgagctgt
     5161 atgcctatat gcactacggc gtgacgcccg atctgttaac caccgccaaa gcgctgggcg
     5221 gcggtttccc ggtcggtgcg ttgttggcaa ccgaagagtg cgcccgcgtg atgaccgttg
     5281 gcactcatgg caccacctat ggcggtaatc cgctggcctc ggcggtggca ggcaaagtgc
     5341 tggagctcat caacacacca gagatgctta atggcgttaa acagcgtcac gactggtttg
     5401 ttgagcgtct taatactatt aatcaccgct atggtttgtt cagtgaagtt cgcggcttag
     5461 gtttgttgat tggctgtgta ctgaatgccg attacgccgg gcaagcgaaa cagatctctc
     5521 tggaagcggc gaaagcaggc gtgatggtac tgattgcggg tggcaacgtg gtgcgttttg
     5581 cgcctgcgct caatgtcagc gaagaagagg tgacgaccgg actggatcgc tttgcagctg
     5641 cttgcgaaca ctttgttagc cgaggttcat catgatggtc atccgtcccg ttgagcgctc
     5701 agatgtctcg gcgctgatgc agcttgccag caaaacgggc ggcggcctga cgtcgcttcc
     5761 cgccaatgaa gccacgcttt cggcgcgtat cgaaagggca atcaaaacct ggcaaggcga
     5821 actgcccaaa agcgagcagg gctatgtgtt cgtgctggaa gatagcgaga caggcaccgt
     5881 ggcggggatt tgcgctattg aggtggcggt tgggctgaac gatccctggt acaactatcg
     5941 cgtcggcacg ttggttcacg cctcaaaaga gctgaatgtc tataacgcat tgccgacgct
     6001 gtttcttagt aacgatcaca ccggcagcag cgagctgtgc acgctgtttc tcgacccgga
     6061 ctggcgtaaa gagggcaacg gctatttgct gtcgaaatcg cgctttatgt ttatggcggc
     6121 ttttcgcgac aagtttaatg acaaagtggt tgctgaaatg cgcggggtga ttgacgaaca
     6181 cggctattca ccgttctggc aaagcctcgg taaacgcttc ttttcgatgg attttagccg
     6241 cgccgatttt ctctgcggca ccgggcaaaa ggcatttatt gcagaactga tgccgaaaca
     6301 tccgatctat acccactttt tatcccagga agcccaggac gtcatcggtc aggtacatcc
     6361 gcaaaccgcg cctgcccgcg cggtgctgga gaaagaaggt tttcgttacc gcaactatat
     6421 cgacatcttc gacggcgggc caacgcttga gtgtgacatc gaccgcgtgc gcgccatccg
     6481 taaaagtcgg ctggtggaag tggcagaagg gcagcctgcg cagggcgatt ttccggcctg
     6541 cctggtcgcc aatgaaaatt atcaccattt ccgcgtggtg ctggcgcgta ccgatccggc
     6601 aaccgagcgt ttgattttaa ccgccgcaca actggatgcc ctcaaatgcc acgcagggga
     6661 tcgcgttcgt ctggtgcgcc tgtgcgcaga ggagaaaaca gcatgacttt atggattaac
     6721 ggtgactgga taacgggcca gggcgcatcg cgtgtgaagc gtaatccggt atcgggcgag
     6781 gtgttatggc aaggcaatga tgccgatgcc gctcaggtcg aacaggcttg tcgggcagcc
     6841 cgtgcggcgt ttccgcgctg ggcgcggctc tcattggctg aacgtcaggt cgttgtcgaa
     6901 cgctttgccg gattgctgga aagcaataaa gccgaattaa ccgcgattat tgccagagaa
     6961 acgggtaagc cgcgctggga agcggcaacc gaagtgacgg cgatgatcaa taaaatcgcg
     7021 atatcaatta aggcgtatca cgttcgtacc ggcgagcagc gtagtgaaat gccggacggt
     7081 gcggcgagcc tgcgacatcg cccgcacggc gtgctggcgg tgtttgggcc gtataatttc
     7141 cctggtcatt tgccgaacgg acatatcgtt ccggcattgc tggcaggtaa caccattatc
     7201 tttaaaccca gcgaactgac accgtggagt ggcgaagcgg taatgcgttt atggcagcag
     7261 gctggcttgc cgccgggcgt gctgaacctg gtgcagggcg ggcgtgaaac gggtcaggcg
     7321 ctgagtgcgc tggaggatct cgacggtttg ctgtttaccg gtagcgccaa taccggctac
     7381 cagctgcatc gccagctctc cggtcagccg gagaaaattc tcgcccttga gatgggcggt
     7441 aataatccgc taattatcga tgaggtggcg gatatcgacg cggctgtcca tctgaccatt
     7501 cagtcggcgt ttgtcacagc cgggcaacgc tgcacctgcg cccgccgttt attgctcaaa
     7561 agcggagcgc agggcgatgc gtttcttgct cgtctggttg ccgtcagcca gcgattaacg
     7621 ccgggcaact gggatgacga accgcagccg tttattggcg ggctgatttc tgaacaggcc
     7681 gcacagcagg tggttactgc ctggcagcaa ctggaagcga tgggcggacg aaccctgctt
     7741 gcgccgcgct tattacaagc agggacatcg ttgctgacac cagggatcat tgaaatgacg
     7801 ggcgttggcg gtgtgccgga tgaagaggtg ttcgggccgc ttttgcgcgt ctggcgttat
     7861 gatacttttg atgaggcgat tcggatggcg aacaacactc gcttcggact cttttgcggt
     7921 ctggtttccc ccgagcggga aaaattcgat caactgttgc tggaggcgcg ggcggggatt
     7981 gttaactgga acaaaccgct tactggtgct gccagtaccg cgccattcgg cggcattggt
     8041 gcttccggta accatcgccc cagcgcctgg tatgccgcag attactgcgc atggcctatg
     8101 gcgagcctgg agtcggactc gttaacattg cccgccacgc ttaaccccgg gctggatttt
     8161 tccgatgagg tggtgcgatg aacgcctggg aagttaattt cgacgggctg gtagggctga
     8221 cgcatcatta cgcgggcctg tcgtttggta atgaagcctc tacccgtcac cgttttcagg
     8281 tgtctaaccc gcgactggcg gcaaaacagg gcttactgaa aatgaaagcc cttgccgatg
     8341 cgggattccc ccaggccgtg atcccgccgc acgagcgtcc gtttattccg gtgctgcgtc
     8401 agttgggatt cagtggtagc gatgagcagg tactggaaaa agttgcacgc caggcaccgc
     8461 actggctttc cagcgtcagt tccgcttcgc caatgtgggt agccaatgcg gcaacgatcg
     8521 cgccatctgc cgatacgctg gatggcaaag tgcatctcac cgttgccaac ctgaacaata
     8581 aatttcaccg ttcgctggaa gcgcccgtca ctgaatcgct gttaaaagcg atttttaagg
     8641 acgaagagaa atttagcgtc cattcggcgt tgccgcaggt agcgttgctc ggtgatgagg
     8701 gggcggcaaa ccacaatcgt ctcggcggtc attacggtga accgggtatg ctactttttg
     8761 tctacgggcg agaagaaggc aatgataccc ggccttcccg ttatccggcg cgacagactc
     8821 gcgaagccag cgaggcggtg gcaaggctta atcaggtgaa tccccaacag gtgattttcg
     8881 cccagcaaaa cccggacgtt atcgaccagg gcgtttttca taatgacgtg attgccgtga
     8941 gtaaccgcca ggtgctgttt tgccaccaac aggcgttcgc tcgccagtca cagttactgg
     9001 caaacctgcg ggcgcgggtg aacggtttta tggcgataga agttccggca actcaggttt
     9061 ccgtgtcgga tgcggtgtct acctatctgt ttaacagcca actgttgagc cgcgatgatg
     9121 gttccatgat gttggtgctg cctcaggagt gtcgggaaca cgccggagta tggggttatc
     9181 tcaatgaact ccttgccgct gacaacccga ttagcgaact aaaagtcttt gacttacgtg
     9241 aaagcatggc gaatggcggc ggcccggcgt gcctgcggtt gcgggtggta ttgacagaag
     9301 aagaacgccg ggcggtgaat ccggcggtga tgatgaacga taccttgttt aatgcgctca
     9361 atgactgggt ggatcgttac taccgcgatc gtcttactgc tgccgatctg gccgacccgc
     9421 aattgctgcg cgaagggcgg gaagcactgg atgtattgag ccaattactg aatctcggtt
     9481 cggtttatcc gttccagcgc gagggagggg gcaatggata attttcttgc tctgacctta
     9541 acgggtaaaa aaccggttat caccgagcga gaaatcaacg gcgttcgctg gcgctggctg
     9601 ggcgatggtg tgctggaact gacgccatta acgccaccgc aaggcgcgct ggtgatttct
     9661 gcggggatcc acggtaatga gacggcacct gtggaaatgc tggacgcgtt gcttggcgcg
     9721 atatctcacg gcgagatccc gttacgttgg cggttgctgg tgatcctcgg gaatcctcct
     9781 gcgctgaagc aagggaaacg ttattgccat agcgatatga accgaatgtt tggcggtcgt
     9841 tggcagctat ttgctgaaag cggagaaacc tgtcgggcgc gcgaactgga acagtgcctg
     9901 gaagattttt atgaccaggg caaagaatct gtgcgttggc accttgatct acataccgca
     9961 attcgtggct ccttgcatcc gcagttcggt gtattaccgc aacgcgacat tccctgggac
    10021 gagaaatttc tgacgtggct gggtgcggcg gggctggagg cgctggtgtt ccatcaggag
    10081 cctggtggta cgtttaccca tttcagcgtc agacattttg gcgcgctggc ctgtacgttg
    10141 gaacttggca aagcgttgcc ctttgggcaa aacgatcttc gccagtttgc agtaactgcc
    10201 agcgcaattg ctgcgctgct atctggtgag agtgtcggta tcgtgagaac accgccgctc
    10261 cgttatcggg tggtttcgca aattactcgc cactcgccgt ccttcgaaat gcatatggca
    10321 agtgacacgc tgaattttat gccgtttgag aaaggaacat tgctggcgca ggacggagag
    10381 gaacgtttta ccgtaaccca tgat
//
LOCUS       DBKEKI010000140        10209 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.141, whole genome shotgun sequence.
ACCESSION   DBKEKI010000140 DBKEKI010000000
VERSION     DBKEKI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.141"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..295
                     /locus_tag="ACUQPX_003455"
     CDS             <1..295
                     /locus_tag="ACUQPX_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHV9058032.1"
                     /translation="GGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGA
                     YRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            313..951
                     /locus_tag="ACUQPX_003456"
     CDS             313..951
                     /locus_tag="ACUQPX_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHV9058033.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1242..1970
                     /gene="mpaA"
                     /locus_tag="ACUQPX_003457"
     CDS             1242..1970
                     /gene="mpaA"
                     /locus_tag="ACUQPX_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HHV9058034.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AAHWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(1945..2910)
                     /gene="ycjG"
                     /locus_tag="ACUQPX_003458"
     CDS             complement(1945..2910)
                     /gene="ycjG"
                     /locus_tag="ACUQPX_003458"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HHV9058035.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3029..3535
                     /gene="tpx"
                     /locus_tag="ACUQPX_003459"
     CDS             3029..3535
                     /gene="tpx"
                     /locus_tag="ACUQPX_003459"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HHV9058036.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3579..5120)
                     /gene="tyrR"
                     /locus_tag="ACUQPX_003460"
     CDS             complement(3579..5120)
                     /gene="tyrR"
                     /locus_tag="ACUQPX_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HHV9058037.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VTCATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5268..6329)
                     /locus_tag="ACUQPX_003461"
     CDS             complement(5268..6329)
                     /locus_tag="ACUQPX_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HHV9058038.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6326..7723)
                     /locus_tag="ACUQPX_003462"
     CDS             complement(6326..7723)
                     /locus_tag="ACUQPX_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HHV9058039.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            7879..8877
                     /locus_tag="ACUQPX_003463"
     CDS             7879..8877
                     /locus_tag="ACUQPX_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHV9058040.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(8988..9893)
                     /gene="ompG"
                     /locus_tag="ACUQPX_003464"
     CDS             complement(8988..9893)
                     /gene="ompG"
                     /locus_tag="ACUQPX_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HHV9058041.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(9938..>10209)
                     /locus_tag="ACUQPX_003465"
     CDS             complement(9938..>10209)
                     /locus_tag="ACUQPX_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HHV9058042.1"
                     /translation="QESLHKPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLY
                     TTVGGHELVVRAGALNDYHAGENITIHFDMTKCHFFDAETEIAIR"
BASE COUNT         2552 a         2747 c         2417 g         2493 t
ORIGIN      
        1 gggaggtgcg acaaaaatgt tgttctcaca tgcacagaaa acgctggcct gggatgcctt
       61 cgcatttact gaagtgttac tgacccagcc ggtgatggta gtagttggtg aaaaagtcgg
      121 tgccttcggt gcttatcgtg atggactgga agtctatggc cgggcgatgg tttcgcagga
      181 tcgtcagtta gtttcgttac ccgatttctc acattatgaa ctatatgaca aaccggaagc
      241 agttcaggag gcgctggcaa aagttattcc ttttttcaac acccatctgg gataagtttc
      301 atgagaaata ttatgaaaaa tgtactgatt cttggtgctg gcggtcagat agcccgccat
      361 gtgattaatc aactggcaga taaacaaacc attaaacaaa ctttatttgc ccgtcagcca
      421 gcaaaaatac acaaacccta tccaactaat agccagatta ttatggggga tgtactgaat
      481 catgcagcac tgaagcaggc catgcaggga caggatattg tgtatgccaa ccttacgggg
      541 gaagatttag atattcaggc aaattcagtg atcgctgcga tgaaagcctg tgatgttaaa
      601 cgtctgatct ttgttctttc cctcggtatt tatgatgaag ttccaggaaa atttggcgaa
      661 tggaataatg cagttattgg tgaaccccta aaaccattcc gcagggctgc ggatgcaatt
      721 gaggcttctg gactggaata tacaattctt cgccctgcct ggctgactga tgaagatatt
      781 attgattatg agcttacctc tcgcaatgaa ccattcaaag gtacgatcgt atcacgtaag
      841 agtgtcgcag cattaattac agatatcatc gataaacctg agaaacacat tggtgagaat
      901 ataggcatca atcaacccgg gactgatggc gataagccat tcttcatgta aatcaattat
      961 atagatacca tcgtactgta ctatgtgcag tacgatttat taaacttatt aaggtgaact
     1021 gatcaatcag atgcgattaa catgtgtaga accagtaaga ataaaatacg ccatgtatat
     1081 aacattacga caattctaat atctgcacat caatgaaaat gcaaacaact tccggcaaaa
     1141 aagtgataca agatcgatga catagcgttg aagaaaagga tttgataatc cgcaaaattt
     1201 gcacaacact gcctgtaata cttcctgaac cagagacgat catgaccgta acccgcccac
     1261 gcgccgaacg cggcgcattt ccgcccggaa cagaacatta cggacgttca ttattgggcg
     1321 cgccgttaat ctggtttcct gcccctgcag ccagccgcga aagtggtttg attctggctg
     1381 gcactcacgg tgatgaaaac tcttcggtcg tcacgctctc ctgtgctctg cggactttga
     1441 caccttcttt gcgccgccat catgtggtgc tgtgtgtgaa tcctgacggc tgccagctgg
     1501 ggttacgggc caatgctaat ggtgtggatt taaacagaaa ctttccggcg gcgcactgga
     1561 aggaaggtga aacggtttat cgctggaaca gcgccgctga agaacgtgat gtcgtattac
     1621 tgacaggtga caaacctggc tccgaacctg aaacccaggc gctgtgccag cttatacacc
     1681 gcattcaacc tgcctgggtg gtctcattcc acgatccact ggcttgtatt gaagatccca
     1741 gacacagcga attaggcgaa tggctggccc agtcgtttga attgcctctg gtaaccagcg
     1801 tcggttatga aacgcctggc tctttcggca gttggtgtgc agatctcaac ttgcattgca
     1861 tcaccgcaga gtttccgccc atctcctccg acgaagccag cgaaaaatat ctctttgcga
     1921 tggcaaactt gctgcgctgg catcctaaag atgcaattcg cccgtcgtga actgaagcgc
     1981 cggttccaca tctaccgcca gccaggtcgg tccgtcaaga tcggcgaaac tgacctgcgg
     2041 caccagcggt aaagcggcgc taatggcacg agaggtacac aacatgcagc ccagcatcag
     2101 actgaaacct tgtgcacgcg cttcagtcgc cagcgccagc gcttccgtca gaccaccggt
     2161 tttatcgagc ttaatgttaa ccatctcata gcgccctttc agcgccttca gattgctacg
     2221 agtatgacaa ctttcatcag cacaaatcgg caacggatga ataaaattct ccagcgccgc
     2281 atcgtcctgc gcaggaagcg gttgttcaag catcgcaacg cctaaatccg ccaatagctg
     2341 gcaacgcgcc gccaaccctt ctgcacgcca ggattcattt gcatcaacga tcagcgtggc
     2401 atcgggcaca gcggtgcgaa ttgccaccat ccgctcactg ataagatggt tatccagctt
     2461 cactttcagt aatttcgcgc ctgcctgcca gagtgttgat gcactattgg ccatctgatc
     2521 aggcgtaccg atgacaaccg tctgtgcagt gataactgtc tcgggaagcg ttatgccgat
     2581 caaatcagcc agcgattgct gctgttttcg cgccgcgaga tcccacaatg cacaatccag
     2641 cgcattacgt gctgcgccgg cagggagaat tttttgcaac tcctcccgtg tcagcccttt
     2701 ctctaattgc ggcacgacgc tcataatttg cgccattacc gaggcatcac tttctccata
     2761 acgcggatac ggcgtgcatt cgccggtgcc tttaataccc tcttcttcca gttcaaccac
     2821 caccacgcgc gcttcactgc gacttccccg ggcaatcaca aacggggtat gtaagggcca
     2881 ggcttcctca aataccttaa cgattctcat ctttactcct tgttgtcgcg gtggtctgcg
     2941 gattaagcgc aaatagcgtt tgctgtgtta ttgacagtta gcataaacta ggtgtgacgt
     3001 taactatatg taaacaggaa agataatcat gtcacaaacc gttcatttcc agggcaaccc
     3061 ggttacagtc gccaattcca tcccgcaggc gggtagcaaa gcgcagactt ttactctcgt
     3121 ggcaaaagat ctgtctgacg tcaccctcgg tcagtttgcg ggtaaacgca aagtgctgaa
     3181 cattttcccg agtattgata ccggtgtttg cgccgcatca gtacgtaagt ttaaccaact
     3241 ggcaactgag atcgacaaca ccgttgtgct gtgtatctct gccgatctgc cgttcgccca
     3301 gtctcgtttc tgcggcgcag aaggtctgaa caacgttatc accctctcca ctttccgtaa
     3361 cgctgaattt ctgcaagcct acggtgtggc aattgctgat ggcccactga aaggtctggc
     3421 agcgcgtgcc gttgtggtta ttgacgaaaa tgacaatgtg attttcagcc agctggtgga
     3481 tgaaatcacc accgagccgg attacgaagc agctctggct gtactgaaag cataagttaa
     3541 tatgcctgat ggtgttgcac catcaggcat attcgcgctt actcttcgtt cttcttctga
     3601 ctcagaccat attcccgcaa cttattggca atcgcggtat gtgaaacgcc gagacgtttt
     3661 gccagtttgc gcgtgctggg ataattgcga taaagctggg ttaataccga gcgttcaaaa
     3721 cggctggtga tttcgtccag cgaaccttcc atcgcatctt cgcccacggc taccgttgcg
     3781 gcgtcataat ccggcaacaa aatatcctgt ggacgcagct cataaccgtc cagttgtgtc
     3841 agtgcgcgat agatagcgtt ctttaactgc cgcacatttc ccggccacgc ataacgtgta
     3901 agtacagtat tcaggtcagc ggccagtttc ggacgcggca cgccctgctc gtcggcaaag
     3961 cgggcgacga acagctcagt taacggcata atgtcctgcg gacagtcacg tagcggcggc
     4021 agattgagcg tcaacacgtt cagacgataa tagagatctt cacggaacac gcctttttgc
     4081 accagttcga ccagattctt ctgcgtagcg caagtcaccc gcacatcgac atgcacctca
     4141 tggtcttcgc caacccgacg gaaagtgcca tcattaagaa aacgcagtaa tttcgcctgc
     4201 atccgtggtg acatttcccc tatttcatcc aacagcaccg aaccaccgtt cgcctgctca
     4261 aagaaccctt tcttcccttc cggagcatga ccaaacagtt cactctcgac cgcatcttct
     4321 ggtatagacg cgcaattcag cgccaggtaa ggtttgcccg ctctggggct tgcctgatgg
     4381 caggcgtagg caaaaagatc tttacctgta cctgtgtcac ccgtaatcag cagcggcgcg
     4441 cttagcatcg ccagtttctg cgcctgttcg acaacgtgct tcattttcgg gctgacggcg
     4501 acaatttggc tgaaggcgct gacgtcctgg gcggcgacat tttgcaactg gcggcccata
     4561 cgaatcgttg atcgcaacat caccaccgca ccggtcagga cgtgttgatc attttcatcc
     4621 tgaagataaa caggcgtaat ctccatcagg aaattctgcc cattaataac gacatgctcg
     4681 ttatgcgaat cttgcggttc gctttccagc caacgtaaaa aattaaagcc gttaatcaat
     4741 tgtgcggcgg tatggttgcg caggcgatcc aatttttgcc caaaaagctg acagctcgcc
     4801 gggttcgcca tatccacttt gcttttcata tcgacagaga gcacaggttc aggcaacgcc
     4861 tccagtaacg cgctcaacgc cagatgctca cgttcggaag gcatccacgg gacagtacgc
     4921 acatcggtaa cacctgcaat acggcgtatt tcggccatca gactgctgaa actctcaaac
     4981 tccagttcag caaaattgag gtagattcgc ccaatgggat caatctcaat accgcgtaaa
     5041 tcaatgcctc ttagcacgag tagatcgagt aattcgcggg tcagaccgag tcggtcttca
     5101 caaaagactt ccagacgcat gggaaccttc acctgaaaaa agaacaatta atatgatgat
     5161 atgacactat ttggatagca ggaaggtttc tgtcaacaat cattgacaca aagacggaaa
     5221 aacgggcact tcggcgtaaa gatatccggt cagcacccga tattgcatta tttttcgctg
     5281 ggcgttttgc ctttttgcag cgtttctttc acctgaccga taagctgacg acggaaatcc
     5341 ccaaggcgag gtttgtcatc gtcaatccac ggcagcgggc ggcaaagctc catagcttta
     5401 atcccgagtc gtgccgtcag aagtccagca ccaattccct gagctgcgcg ggtagacaaa
     5461 cgagcagcga gatcttgcga catccagtcc atccccactt cgcgcaccag ttcgctggct
     5521 ccggcaaaag cgatattcag caataccagc ttaaacagac gcaaacggct gtaatacccc
     5581 agttcaatgc catacagcgt ggcaatgcga ttaattaaac gcagattgcg ccaggcgata
     5641 aacgccatat cgaccaacgc cagcgggctg accgcaatca tcaacgttga ttccgccgcc
     5701 gaacggctga tttcgcgccg cgcctgggca tctaaaactg gctggaccaa atgcgcatac
     5761 aggctgacca cttcacgatc gttctgcgtt tcatggattg aggcatacca gcgttgcagc
     5821 gccggatgcg actgatcaat acccgcctgc tgcgccagtt tttcgcaaaa tgcgcgacct
     5881 ttgcccgtgc cgtggctatg caataaatca cgcgcttcgt cgcgttcatg ggcgcgctgt
     5941 cgcaagcgcc ataagcgccg ccactctgtt accacagaac ctacgccagc gccgataatc
     6001 aatgccccag cggcacatcc acccagcgct acccagtcct gggtttgcca ggcattcatt
     6061 gtccactgta taccctgccc gacaacgctt gcgccaaaca gagccagccc gcccatcacc
     6121 attttgcgcc acaggctgcg tttcggacgt aacgctgcgt ccattaccgc ttcgacttgc
     6181 ccctcttctt cctgcgcttc gtcgagcgtg gccggggcaa aattttgcgc ctgattttcg
     6241 tcaaaggtct gctgcgccct gaattttgga ttctgttcga cctccagcgg accgtcgaaa
     6301 tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc tcctattaaa aattccagcg
     6361 cggcatcaag acgaatatgc ggcagcggtt tgtcgacatc cattacctgc gggcgaaacg
     6421 cttcaaactg gaacccttgc ttgtcccaga acgcctgacc gggcaaacgc gcaggaactt
     6481 caccggggta aacggtgaga ggcgcaccat cgctgagccg gttaccgcgc aatgccggaa
     6541 ttttctcgcc gttgacgtca ataatgccgc tggtagtcgc ctgcaccgag gccagcccca
     6601 ggcaatccat actaatccct tcaaacgccg cattttgcca ggcatcctga atcagttgtt
     6661 gcagtaatga aaccatattg gcgtgctgat cgaccgttac atggtccgct ttcgtcgcgg
     6721 caaacagcag tttgtcgata accggcgaga ataagcggcg aaacagcgtg cgctgcccgt
     6781 agtggaaact ttgcatcagc tgcgtcagtg ccagacgcat atcattaaat gcctgtggcc
     6841 cactgttgag aggttgcagg caatccacca gcacaatctg gcggtcaaag cgcagaaaat
     6901 gattcttata gaaccccttc accactttct cgcagtaata attaaaccgc tcgcgcagca
     6961 ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga ctcgccccag gcatcgacat
     7021 ccggccacgg gaagaattgc agcgcgggcg cacctgccat atctcccggc aagacaaagc
     7081 gcccaggctg aataaagtgc agcccctgct gtttacagtg gtggagataa tcggtccaca
     7141 cggcggcaat gtctgccagc cggttttcgt cggcaggcgc tagcgggtca agtccttcgc
     7201 acatcattcg ccatttcgcc gaccattcgc cgcgctgacc attgagtaag cccgtcatct
     7261 ggcgcgacca gcttaagtaa tcctgcgcca gcatcggcag gtcgagcaac cattcgccgg
     7321 gataatccac aatttccaga tacagcgtgg aggtatcctt aaagtggcgc agcagcgaat
     7381 cgttcgattt aaaacgtaac gccaggcgaa tttcactgac gccgcgcgta ggcgtcggcc
     7441 aggcgggagg atcaccatat aactgcgcca gtccttcatc ataggtgaag cgcggaatgc
     7501 caaagtcacg ctggggaatg cgttttacgc ccagcaagcg ctcttcacgc accgcgctta
     7561 acagcggcaa acgtgctccg gcatgaatat tgagcaactg attaaccatc gcggtgataa
     7621 acgctgtttt gccgctgcgg ctaagtccgg ttacagcgag gcgcagatgt ctgtcgacac
     7681 cccgattcac cagcgcattg agttcatttt taagtcgctt cattgctgtc ctgtgctgct
     7741 ctgaagacca gaaaaaaaat cattcgtaag gatatgaatg tgggggccag gccattgatt
     7801 atcaactgcc cgtgttgtca tcgacgggcg aactccataa gatgtgcagc attcacgttc
     7861 ggacggagtt cctgacctat gtcccctact atttatgata ttgccagggt tgcaggcgta
     7921 tcaaaatcca ccgtatcacg cgtgctgaat aagcaaacta atatctcccc ggaagcacgc
     7981 gaaaaagtgt tacgggccat tgaagaatta cagtatcagc caaacaagct ggcccgcgcg
     8041 ctgacctctt cgggttttga tgccattatg gtgatttcta cccgttcgac caaaactacg
     8101 gcgggtaatc cgtttttctc tgaagttttg catgccatca ccgccaaagc ggaagaagaa
     8161 ggtttcgacg tgatattaca aacgtcgcac aatccggcag aagatctgca aaaatgcgaa
     8221 agcaaaatta aacagaaaat gattaaaggc attattatgc tcagttcgcc agcggatgag
     8281 tcattttttg cccaactcga taaatacgat attccggtgg tggtaattgg caaagttgaa
     8341 ggtcaatatg cccatgttta ttctgtcgat accgataatt ttggtgacag cattgcgttg
     8401 accgatgcat taattgaaag tgggcatcaa aatattgcct gcctgcatgc tccgcttgat
     8461 gtccatgttt cggttgatcg cgttaatggc tataagcaaa gtctgtccgc gcataatatt
     8521 gcagtgcgtg ataaatggat tgttgacggc ggttataccc atgaaacagc actgcaagca
     8581 gcacggcaat tattaagtca gtcaccgttg cctgaggcag tgtttgccac tgacagcctg
     8641 aaattaatga gcatttatcg tgcggcggca gagaaaaata ttgctattcc gcagcagtta
     8701 gcggtggtgg gttatagtaa tgaaacgctg tcgtttattt taacgcctgc accaggcggc
     8761 atcgatgttc cgacgcagga gttaggtcaa caaagctgcg agttattatt ccgcttaatt
     8821 tcgggaaaac cgtcaccaca gaatattacc gttgccacgc atatgacgct gaaataaata
     8881 ccagctacct tgcctgaatg ccggatgcga tgctggcgca tcttatccgg cctacattta
     8941 tagatcggat aaagtactcg cgccgcatcc ggcaatttag cccattatca gaacgagtaa
     9001 tttacgccga cacctgcata atggaattta tcgctgtcgc cttcgtcgtg atcctgccac
     9061 tcaaacgcgt attccagcga aacggaaagt ccgttctgga aatcataacc gtaaaataaa
     9121 cccacacggt taaaatcatg gccttcacgt tcaatatcat cctgccagtc ccagttactc
     9181 cagcgatcca gcccaatgcg cgtatacggc gtcaccgagt ggttgccgag cgtcagcggc
     9241 aaataggcgc gaatttcttg tgtggaaaac tcaccgttat tgcggctgtc gtccatattg
     9301 aagccgcgct cgagataata gttcactcgc aaagcaaccg tttcgttgaa ggtatattgc
     9361 agacctgttt ccgtttcgac acgggtatca gcgtaaccgg tcgtgttcaa atcgttggca
     9421 aatttataca tcgacaacca accgttgaaa cgtaaatcat cagtcagttt cacatcccag
     9481 tctggcgcga ttttccagcg ctgcatattc gccgtgtctt tgcccggttc atcaacgtag
     9541 tgataaccat aattacggaa accgccggtc aggccgaaac tgaaatcatc gctttcgagg
     9601 aactgataat gcacctccag ctccgggcga tcaaaccacg ttccacgttt acccgcgcta
     9661 taatctaccg gcccttcctg ataataggcc agagcaattc tccacggccc gttggcggca
     9721 ttaaaataga ctgaaggctc cgccagccca tccatatctt cgccataacc ctcgacgttt
     9781 tctatttcgt acatcgcacc gatattaaag tgccagtcgt tcttttcctc ggcctgtgcg
     9841 caggccattc ccgcacacat caccagtgcg gtacagggta ataacttttt cattattgtt
     9901 ccttgtatta tcctggggga atgccttccc cctgtattta gcgaattgct atttccgttt
     9961 ctgcatcaaa gaaatgacat ttcgtcatat caaaatgaat agtgatattt tctcctgcat
    10021 gataatcatt taacgcacca gcacggacca ctaactcgtg ccccccaacc gtggtgtaga
    10081 gcataaattc cgcaccggtt aattctgcca cgctaatttt ggcggaaatg ttattttcct
    10141 cttgcgcgtc cggatgaata tcttccgggc gtattcccat cacgatgggc ttatgcaaac
    10201 tttcctgtg
//
LOCUS       DBKEKI010000141        10206 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.142, whole genome shotgun sequence.
ACCESSION   DBKEKI010000141 DBKEKI010000000
VERSION     DBKEKI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10206
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.142"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            333..473
                     /locus_tag="ACUQPX_003466"
     CDS             333..473
                     /locus_tag="ACUQPX_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HHV9058043.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            476..643
                     /gene="yciX"
                     /locus_tag="ACUQPX_003467"
     CDS             476..643
                     /gene="yciX"
                     /locus_tag="ACUQPX_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HHV9058044.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            757..852
                     /gene="ymiC"
                     /locus_tag="ACUQPX_003468"
     CDS             757..852
                     /gene="ymiC"
                     /locus_tag="ACUQPX_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HHV9058045.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            1016..3691
                     /gene="acnA"
                     /locus_tag="ACUQPX_003469"
     CDS             1016..3691
                     /gene="acnA"
                     /locus_tag="ACUQPX_003469"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HHV9058046.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYVLAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3755..4345)
                     /gene="ribA"
                     /locus_tag="ACUQPX_003470"
     CDS             complement(3755..4345)
                     /gene="ribA"
                     /locus_tag="ACUQPX_003470"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HHV9058047.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHIALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            4515..5279
                     /gene="pgpB"
                     /locus_tag="ACUQPX_003471"
     CDS             4515..5279
                     /gene="pgpB"
                     /locus_tag="ACUQPX_003471"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HHV9058048.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5428..5736
                     /gene="lapA"
                     /locus_tag="ACUQPX_003472"
     CDS             5428..5736
                     /gene="lapA"
                     /locus_tag="ACUQPX_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HHV9058049.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            5743..6912
                     /gene="lapB"
                     /locus_tag="ACUQPX_003473"
     CDS             5743..6912
                     /gene="lapB"
                     /locus_tag="ACUQPX_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HHV9058050.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            7105..7842
                     /gene="pyrF"
                     /locus_tag="ACUQPX_003474"
     CDS             7105..7842
                     /gene="pyrF"
                     /locus_tag="ACUQPX_003474"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HHV9058051.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7842..8168
                     /gene="yciH"
                     /locus_tag="ACUQPX_003475"
     CDS             7842..8168
                     /gene="yciH"
                     /locus_tag="ACUQPX_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HHV9058052.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(8294..8512)
                     /gene="osmB"
                     /locus_tag="ACUQPX_003476"
     CDS             complement(8294..8512)
                     /gene="osmB"
                     /locus_tag="ACUQPX_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HHV9058053.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8781..9530)
                     /locus_tag="ACUQPX_003477"
     CDS             complement(8781..9530)
                     /locus_tag="ACUQPX_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HHV9058054.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(9620..9793)
                     /gene="yciZ"
                     /locus_tag="ACUQPX_003478"
     CDS             complement(9620..9793)
                     /gene="yciZ"
                     /locus_tag="ACUQPX_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HHV9058055.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            9831..9959
                     /gene="ymiD"
                     /locus_tag="ACUQPX_003479"
     CDS             9831..9959
                     /gene="ymiD"
                     /locus_tag="ACUQPX_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HHV9058056.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            complement(9941..>10206)
                     /locus_tag="ACUQPX_003480"
     CDS             complement(9941..>10206)
                     /locus_tag="ACUQPX_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HHV9058057.1"
                     /translation="FPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEG
                     VESAKEDAFLTKNGINERQGFLFAKPMPAVAFERWYKCYLKRA"
BASE COUNT         2579 a         2369 c         2625 g         2633 t
ORIGIN      
        1 tgccggaaaa ataatttctt gcgttatttt cggcaccttt tatgtagcga aggtgccgga
       61 atatattctc ttttgttact tatttctata ccgtgcacaa tatttttatc cctcatattt
      121 atagggtaaa ttacctgtaa agtagtgtct aattacccgc ctcgcaaatt ccagttttca
      181 aatatctatt tcaggtcaaa agattgaatt attcactgga gacgattcgt aaattcgctg
      241 gatttttgac taaagtttct ttaggatttg tctcatcgta tgattaatta caccaatcat
      301 tcaacgtctt gctgataagt gatggcgatt atatgaggtt agcaatgcct tctggaaatc
      361 aggaaccccg ccgtgatcct gaattaaaac gtaaagcctg gctggcggtt tttcttggtt
      421 ctgcactttt ctgggtggtt gtcgcactgc tgatttggaa agtgtgggga taactatggt
      481 cggtcaggag caactggagt cgtcaccatt atgccagcat agtgacaatg aaccagaaac
      541 gaaacgggaa tgttccgtcg ttattccaga cgactggcaa ctaacatcgc agcagcaagc
      601 ctttatagaa ctgtttgctg aagatgatca gccgaaacaa taattatcat cattcttatt
      661 acccattttt aatgaattaa agggctttta atacaccgca gcaataacag cttgagttat
      721 ctcaacacaa aataataacc gttaagggtg tagcctatga tcaacacaaa tatgaaatat
      781 tggtcctgga tgggcgcgtt ttctctgtcg atgctcttct gggccgaact cctctggatc
      841 attactcact gatccttgac cccgctgcgg cggggttgtc atttgctttg ccacaaggtt
      901 tctcctcttt tatcaatttg ggttgttatc aaatcgttac gcgatgtttg tgttatcttt
      961 aatattcacc ctgaagagaa tcagggcttc gcaaccctgt cattaaggag gagctatgtc
     1021 gtcaacccta cgagaagcca gtaaggacac gttgcaggcc aaagataaaa cttaccacta
     1081 ctacagcctg ccgcttgctg ctaaatcact gggcgatatc acccgtctac ccaagtcact
     1141 caaagttttg ctcgaaaatc tgctgcgctg gcaggatggt aactcagtta ccgaagagga
     1201 tatccacgca ctggcgggat ggctgaaaaa tgcccatgct gaccgtgaaa ttgcctaccg
     1261 cccggcaagg gtgctgatgc aggactttac cggcgtacct gccgttgttg atctggcggc
     1321 aatgcgcgaa gcggttaaac gcctcggtgg cgatactgca aaggttaacc cgctctcacc
     1381 ggtcgacctg gtcattgacc actcggtgac cgtcgatcgt tttggtgatg atgaggcatt
     1441 tgaagaaaac gtacgcctgg aaatggagcg caaccacgaa cgttatgtgt tcctgaaatg
     1501 gggaaagcaa gcgttcagtc ggtttagcgt cgtgccgcca ggcacaggca tttgccatca
     1561 ggttaacctc gaatatctcg gcaaagcagt gtggagcgaa ttgcaggacg gtgaatggat
     1621 tgcttatccg gatacactcg ttggtactga ctcgcacacc actatgatca acggccttgg
     1681 cgtgctgggg tggggcgtgg gtgggatcga agcagaagcc gccatgttag gccagccggt
     1741 ttccatgctt atcccggacg tagtgggctt taaacttacc ggaaaattac gtgaaggtat
     1801 taccgccaca gacctggttc tcactgttac ccaaatgctg cgcaaacatg gcgtggtggg
     1861 gaaattcgtc gaattttatg gtgatggtct ggattcacta ccgttggcgg atcgcgccac
     1921 cattgccaat atgtcgccag aatatggtgc cacctgtggc ttcttcccaa tcgatgcagt
     1981 aaccctcgat tacatgcgtt taagcgggcg cagcgaagat caggtcgagt tggtcgaaaa
     2041 atatgccaaa gcgcagggca tgtggcgtaa cccgggcgat gaaccgattt ttaccagtat
     2101 gttagaactg gatatgaatg acgttgaagc gagcctggca gggccaaaac gcccacagga
     2161 tcgcgttgca ctgcccgatg taccaaaagc atttgccgcc agtaacgaac tggaagtgaa
     2221 tgccacgcat aaagatcgcc tgccagtcga ttatgttatg aacggacatc agtatcagtt
     2281 acctgatggc gctgtggtca ttgctgcgat aacctcgtgc accaacacct ctaacccaag
     2341 tgtgctgatg gccgcaggct tgctggcgaa aaaagccgta actcttggcc tcaagcggca
     2401 accgtgggtc aaagcgtcgc tggcaccggg ttcgaaagtc gtttctgatt atctggcaaa
     2461 agcgaaactg acaccgtatc tcgacgaact ggggtttaac cttgtgggat acggttgtac
     2521 cacctgtatt ggtaactctg ggccgctgcc cgatcctatc gaaacagcaa tcaaaaaagg
     2581 cgatttaacc gtcggtgcgg tgctgtccgg caaccgtaac tttgaaggcc gtatccatcc
     2641 gctggttaaa actaactggc tggcctcgcc gccgctggtg gttgcctatg tgctggcggg
     2701 aaatatgaat atcaacctgg cttctgagcc tatcggccat gatcgcaaag gcgatccggt
     2761 ttatctgaaa gatatctggc catcggcaca agaaattgcc cgtgcggtag aacaagtctc
     2821 cacagaaatg ttccgcaaag agtacgcaga agtttttgaa ggcacagcag agtggaagga
     2881 aattaacgtc acacgatccg atacctacgg ttggcaggag gactcaacct atattcgctt
     2941 atcgcctttc tttgatgaaa tgcaggcaac accagcacca gtggaagata ttcacggtgc
     3001 gcggatcctc gcaatgctgg gggattcagt caccactgac catatctctc cggcgggcag
     3061 tattaagccc gacagcccag cgggtcgata tctacaaggt cggggtgttg agcgaaaaga
     3121 ctttaactcc tacggttcgc ggcgtggtaa ccatgaagtg atgatgcgcg gcaccttcgc
     3181 caatattcgc atccgtaatg aaatggtgcc tggcgttgaa ggggggatga cgcggcattt
     3241 acctgacagc gacgtagtct ctatttatga tgctgcgatg cgctataagc aggagcaaac
     3301 gccgctggcg gtgattgccg ggaaagagta tggatcaggc tcaagccgtg actgggcggc
     3361 aaaaggacct cgtctgcttg gaattcgtgt ggtgattgcc gaatcgtttg aacgaattca
     3421 ccgttcgaat ttaattggca tgggcatcct gccgctggaa tttccgcaag gcgtgacgcg
     3481 taaaacgtta gggctaaccg gggaagagaa gattgatatt ggcgatctgc aaaatctaca
     3541 acctggcgcg acggttccgg tgacgcttac gcgcgcggat ggtagccagg aagtcgtacc
     3601 ctgccgttgt cgtatcgaca ccgcgacgga gttgacctac taccagaacg acggcatttt
     3661 gcattatgtc attcgtaata tgttgaagta acaagtattt gcttgccggt tattttgctt
     3721 ccggcaagca aatgaattac acaatgcaag agggttattt gttcagcaaa tggcccattt
     3781 tctcggcttt ggtatcgaga taatgttcgt tattggggtt acgacctaca atcaatggta
     3841 cgcgttcaac aatattaatc cctgcttcgg tcagaatttc gacttttttc gggttattgg
     3901 ttaacaagcg gacttcattg acgccaagga gtttgaacat atcagcgcaa agagtgaagt
     3961 cgcgctcatc agcggcgaag cctaactggt ggttagcctc tacggtatcg taaccttgat
     4021 cctgcagtgc gtaagcgcgg attttattca gcagaccaat gttacgacct tcctgacggt
     4081 gatacagcaa aataccacgg ccttcctcgg caatttgcgt caatgccgct tcgagctgga
     4141 agccacaatc gcagcgcaag ctgaacaggg cgtcaccggt cagacattcg gaatggacgc
     4201 gcgcaagtac cggggtatgc ccggaaatat cgccatagac tagcgcgata tgatcgtgtc
     4261 cggttgccag ttcttcaaat cccaccatca ggaaatcgcc ccatggggtt ggcagtttgg
     4321 cttctgccac acgtttaagc tgcatgaaat tctccagata atgctggttc tgtattggct
     4381 tattttgcca taacgagaag gggttcacct aatcacgacg cgtcgatcgt tcacggaatg
     4441 gcacaaatct gtcaattttt cttggaactg gcattttcag ttatgattgt gggacttatc
     4501 aaaaaggaga ggccatgcgt tcgattgccc gacgtaccgc agtgggagct gcactattgc
     4561 ttgtcatgcc agtagccgta tggatttctg gctggcgttg gcaacctgga gaacaaagtt
     4621 ggctactaaa agcggctttt tgggttactg aaactgtcac ccagccctgg ggcgtcatta
     4681 cacatttgat tttgttcggc tggtttctct ggtgtctgcg ttttcgcatt aaggctgcct
     4741 ttgtattatt tgccattctg gcggcggcaa tccttgttgg acaaggcgtt aaatcctgga
     4801 tcaaagacaa agtccaggaa ccacgacctt ttgttatctg gttggaaaaa acacatcata
     4861 ttccggttga tgagttctac actttaaagc gagcagaacg cggaaatcta gtaaaagaac
     4921 agttggctga agagaaaaat attccacaat atttgcgttc acactggcag aaagagacgg
     4981 ggtttgcctt tccttccggt cacacgatgt ttgctgccag ttgggcactg ctggccgttg
     5041 gtttgctgtg gccgcgtcgg cgaacgttaa ccattgctat cttgttggtc tgggcaacgg
     5101 gagtcatggg aagccgcctg ctgctcggga tgcattggcc acgcgatctg gtggtagcta
     5161 cgttgatttc gtgggcgctg gtggcggtgg caacgtggct tgcgcaacga atttgtgggc
     5221 cattaacacc acctgcggaa gaaaatcgcg aaattgcaca acgagaacaa gaaagttaac
     5281 gctggttgat tttccgaatt taggccttaa atcatcaaca atgcgcgtgg atgccatttc
     5341 gcagacggcg cgaaaatggt actttaaagg gctattgcgg taagttgacc ataatttatt
     5401 cgctctaacc acataacggg aagtaatgtg aaatatttac tcattttctt actggtgtta
     5461 gcgatcttcg tgatttcggt cacgttgggt gcgcagaacg atcaacaggt gacgtttaat
     5521 tatctgttag cgcaagggga gtaccgtatt tccacattgc tggcggtatt gtttgctgcg
     5581 gggtttgcta tcggttggtt gatttgtggc ctgttctggc tgcgagttcg tgtttccctg
     5641 gcgcgcgctg aacgtaaaat aaagcgactg gaaaaccagc tttcacccgc gactgacgtg
     5701 gctgtagtgc cgcactcgtc agcggcgaag gaataacttt ctatgctgga gttgttgttt
     5761 ctgcttttgc ctgtagccgc tgcctatggc tggtatatgg gccgcagaag tgcgcaacaa
     5821 aacaagcaag atgaagccaa ccgcttgtcg cgtgattacg tagcgggggt taacttcctg
     5881 cttagtaatc aacaggataa agcggtagac ctgtttctcg atatgcttaa agaggataca
     5941 ggcaccgttg aagcccacct tacgctcgga aacctgttcc gttcgcgtgg cgaagttgat
     6001 cgcgctattc gcatccatca gaccctaatg gaaagcgcct cgctgaccta tgaacagcgt
     6061 ctgttggcga ttcaacaact ggggcgtgat tacatggccg ccgggttata cgaccgcgcg
     6121 gaagacatgt tcaatcagct gaccgatgaa actgacttcc gcattggcgc gctgcaacag
     6181 ttgctacaaa tctaccaggc taccagtgag tggcagaaag caattgatgt tgccgaacgc
     6241 ctggtgaagc tgggtaaaga taaacagcgc gtcgaaattg cccatttcta ctgtgagtta
     6301 gccctgcagc atatggccag cgacgatctc gatcgtgcca tgaccttgct aaaaaaaggg
     6361 gcggcggcag ataaaaacag cgcccgcgta tccatcatga tgggacgcgt gtttatggcg
     6421 aaaggagaat acgccaaagc cgtcgaaagt ttgcaacgcg tcatatccca ggacagagaa
     6481 ctggtcagcg aaacgctgga aatgttgcaa acctgctatc agcagttggg taaaactgcc
     6541 gaatgggcag aattcctgca acgcgcggtg gaagagaaca ccggtgccga tgctgaattg
     6601 atgcttgctg atatcatcga agcgcgcgac ggtagtgagg ctgcacaggt ctatattaca
     6661 cgccagcttc agcgtcatcc gaccatgcgt gtattccata agctaatgga ttaccactta
     6721 aatgaagcgg aagaagggcg cgccaaagag agtctgatgg tgctgcgtga catggttggc
     6781 gagaaggtgc gtagtaagcc tcgttatagc tgccagaaat gtggttttac cgcatacacc
     6841 ctctactggc attgtccgtc ttgtcgtgcc tggtcaacta ttaaaccgat tcgcggtctt
     6901 gatggcctgt aatttttaaa aaaatccgac tttagttaca acatactagt tattgatgtc
     6961 ccattgtgct ccggcaacga cggagcagaa aaagcctgct aggggaaaaa tcgcaactgt
     7021 taatttttta tttccacggg tagaatgctc gccgtttacc tgtttcgcgc cacttccggt
     7081 gcccatcatc aagaaggtct ggtcatgacg ttaactgctt catcttcttc ccgcgctgtt
     7141 acgaattctc ctgtggttgt tgcccttgat tatcataatt gtgatgacgc gctgtccttt
     7201 gtcgacaaga tcgacccacg cgattgtcgt ctgaaggtcg gtaaagagat gtttacattg
     7261 tttgggccac agtttgtgcg cgaacttcaa cagcgtggtt ttgatatctt tcttgacctg
     7321 aaattccacg atatccccaa cactgcagcg cacgctgtcg ctgctgcagc tgacttaggc
     7381 gtgtggatgg tgaatgttca tgcctccggt ggggcgcgta tgatgaccgc agcgcgtgag
     7441 gcactggttc cgtttggcaa agatgcaccg cttttgattg ctgtgacagt gttgaccagc
     7501 atggaagcca gcgacctggc cgatcttggc gtgacactgt cacctgcaga ttatgcagaa
     7561 cgtctggcag cactgacgca aaaatgtggc cttgatggtg tggtgtgttc tgctcaggaa
     7621 gctgtgcgct ttaaacaggt attcggtcag gagttcaaac tggttacacc gggcattcgt
     7681 ccgcagggga gtgaagctgg tgaccagcgc cgcattatga cgccagaaca ggcgttggcg
     7741 gctggtgttg attatatggt gattggtcgc ccggtaacgc aatcggtaga tccagcgcag
     7801 acactgaaag cgatcaacgc ctctttacag cggagtgcat gatgagtgat tccaacagcc
     7861 gtctggtcta ctcaacggaa accgggcgta ttgatgagcc caaagcggcc ccagtacgcc
     7921 ctaaaggcga tggagcggtg cgtattcagc gtcagaccag tggacgtaaa ggtaagggcg
     7981 tttgcctgat taccggtatc gatctcgatg atgccgaact gacaaaactt gcagcggaac
     8041 tgaagaaaaa atgcggctgc ggcggagcag ttaaagatgg agttattgaa atccagggcg
     8101 ataagcgtga tttattaaaa tcactacttg aagcgaaagg gatgaaggta aaactcgcag
     8161 gcggttaaca taaaaagcca cggatatatc cgtggctttc gaatatttta ctgtgtgtat
     8221 tattcagtta tttcgtactg aacagaatca agcgtattat aattgcgagt cgataatcga
     8281 catttattta cgattattta ccgacctggt gaccaataac accaccgaca gctgcaccac
     8341 ctaatgtacc caacgtactg ccatcggtca gtactgcacc gcctaatgcc cctgcacccg
     8401 cgccgattgc ggtgttgcgg tcccgtttag accagttaga acaggcactc agagacattg
     8461 ccaaagtaat tgccagaaca gccgcggtca tttttttgct cgttacaaac ataatactct
     8521 ctcctgaatt tatgattcac ggaagtaagc tctctataac tataatagct aagaataagc
     8581 ctggtgaaat tattccgtga aatctgctcg cgcgcagggt tatatcacgc aggtgataat
     8641 tacttcctgt tatatcgctg ataataattt tatatcttga gagtgttaat aacaggtaaa
     8701 tagtcttaat tatcaaccag gaatcatctt agagcggatg atttgccaaa ctgcaaatca
     8761 tccgtagaga agggaaatgg ttaaatgtca acgacgtgaa cggtgagttt actgtgctca
     8821 agatgcatca gatcgctggc gcgtattttc gaatcggtaa tcacgcgatt gaatcgctca
     8881 acgggaccga tggagtatga atgtacagca ccaaatttcg agctgtcagt caggactatc
     8941 gcttcgcact ctttttccag cacggcattg accacatcgg tgcgcatcat atcgcgaccg
     9001 gtgaatccag tttcaggttg ccagccatca ataccaataa atgctttgct gaaatgtacc
     9061 tgctggatgc actggcgtgt caaagggcca accatacttt cgcttttttt ctggtacacg
     9121 ccaccgagca aaatgacttc acaaggcgcg tctttcagca aatgcgcgat gtagctgctg
     9181 accgtgatga tagtgacatt tttcttctgc tcccccagag tccgggcgag cagggcattg
     9241 ctgctgccat tttcgataaa gatggtttcg cccggttgaa ccagtgacgc ggcaaactcg
     9301 gcgagttcac gcttcagcgt atagttgctc atcatacggg tttcgacgtc atcactatca
     9361 agcgaaactg caaagccatg tgcacggcgg aggtaactca gtttttcgag ggtgttgaga
     9421 tcctggcgaa tggtaacttc agaaactcca gtggcttttg ccagatcggt tacgctaacc
     9481 tgaccctggt caatgaccat ctgtagaata gtttgttgtc gggaattcat cgctgtaatt
     9541 cttataacgt tataatacta aataaaaaaa tactgacgcg ccgggcatcc tcaaatagga
     9601 aaacctgaag gcacgattgt taaatctccc acggtgtctt cgggatgtct ggcgtagatt
     9661 cagcaacctg gcgcaagagt tctgctttaa gtatctcgag attatggatc gcagagtggt
     9721 aatcgcctgc cacaaggata tctaacacaa tatcaatacg ttcggcaacg cgctgggcat
     9781 caaattcaga catcaaggac atccttatag caaaaggggg aaaatgaata atgcaaaaat
     9841 tgccgctaaa agagaagtgt ttaacagcaa cggctaatta tcatccagga atacgatata
     9901 taatgacggg atatagcgct aagtatatat attcatctac ttatgcgcgc ttcagatagc
     9961 atttatacca gcgttcgaag gcgacggcgg gcatcggttt ggcaaacaaa aatccttgcc
    10021 gctcattgat cccgttcttg gttaaaaaag catcttcctt tgcactctct acaccttcgg
    10081 cgatcacctg aagattcaat gcctgggcca cagcgacgat cgcccggacc agtgactgcg
    10141 agacaggttg tttgtgaata tctcgaacaa aaacctggtc aagtttgatg gcatcgatcg
    10201 gaaagc
//
LOCUS       DBKEKI010000142        10134 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.143, whole genome shotgun sequence.
ACCESSION   DBKEKI010000142 DBKEKI010000000
VERSION     DBKEKI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.143"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..373
                     /locus_tag="ACUQPX_003481"
     CDS             <1..373
                     /locus_tag="ACUQPX_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HHV9058058.1"
                     /translation="KLVLPSWKFALAQMLISSVNWMVMGAIIWLLLGQSVNYFFVLGV
                     LLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGSIIAALLAYRVLYYFIPLLLAL
                     ICYLLLESQAKKLRAKNEAAM"
     gene            complement(409..1122)
                     /locus_tag="ACUQPX_003482"
     CDS             complement(409..1122)
                     /locus_tag="ACUQPX_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HHV9058059.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPSSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAITITLVWQRHTRFFH"
     gene            complement(1327..2031)
                     /locus_tag="ACUQPX_003483"
     CDS             complement(1327..2031)
                     /locus_tag="ACUQPX_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HHV9058060.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(2168..2620)
                     /gene="moaE"
                     /locus_tag="ACUQPX_003484"
     CDS             complement(2168..2620)
                     /gene="moaE"
                     /locus_tag="ACUQPX_003484"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HHV9058061.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(2622..2867)
                     /gene="moaD"
                     /locus_tag="ACUQPX_003485"
     CDS             complement(2622..2867)
                     /gene="moaD"
                     /locus_tag="ACUQPX_003485"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HHV9058062.1"
                     /translation="MIKVLFFAQVRELVGADATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(2860..3345)
                     /gene="moaC"
                     /locus_tag="ACUQPX_003486"
     CDS             complement(2860..3345)
                     /gene="moaC"
                     /locus_tag="ACUQPX_003486"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HHV9058063.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(3348..3860)
                     /gene="moaB"
                     /locus_tag="ACUQPX_003487"
     CDS             complement(3348..3860)
                     /gene="moaB"
                     /locus_tag="ACUQPX_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HHV9058064.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREFEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(3882..4871)
                     /gene="moaA"
                     /locus_tag="ACUQPX_003488"
     CDS             complement(3882..4871)
                     /gene="moaA"
                     /locus_tag="ACUQPX_003488"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HHV9058065.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(4859..5003)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            5268..6176
                     /locus_tag="ACUQPX_003489"
     CDS             5268..6176
                     /locus_tag="ACUQPX_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HHV9058066.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASTMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(6368..8389)
                     /gene="uvrB"
                     /locus_tag="ACUQPX_003490"
     CDS             complement(6368..8389)
                     /gene="uvrB"
                     /locus_tag="ACUQPX_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HHV9058067.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(8968..9645)
                     /gene="bioD"
                     /locus_tag="ACUQPX_003491"
     CDS             complement(8968..9645)
                     /gene="bioD"
                     /locus_tag="ACUQPX_003491"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HHV9058068.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTV
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGELPWLAENPENAATGK
                     YINLALL"
     gene            complement(9638..>10134)
                     /locus_tag="ACUQPX_003492"
     CDS             complement(9638..>10134)
                     /locus_tag="ACUQPX_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HHV9058069.1"
                     /translation="HYLAGDIESLPLATATFDLAWSNLAVQWCGNLSTALRELYRVVR
                     PGGVVAFTTLVQGSLPELHQARQAVDERPHANRFLPPDEIEESLNGVRYQHHIQLITL
                     WFDDALSAMRSLKGIGATHLHEGRDPRILTRSQLQRLQLAWPQQQGRYPLTYHLFLGV
                     IARE"
BASE COUNT         2479 a         2661 c         2498 g         2496 t
ORIGIN      
        1 aaagctggtg ctgccttcat ggaaattcgc cctcgctcag atgctgattt ccagcgttaa
       61 ctggatggta atgggggcga ttatctggct gttacttggt caaagcgtga actatttctt
      121 tgtactgggc gtgttactgg ttagtagtat tgctggcgtc atcgtgcata ttccggcggg
      181 gatcggtgtg ctggaagcgg tgtttatcgc actactggct ggggagcata cctcgaaagg
      241 gagcattatc gccgccctgc tcgcttatcg cgtcctgtat tactttatcc cgctgctgct
      301 ggcgctgatc tgttacctgc tactggaaag ccaggcgaag aagctacggg cgaaaaatga
      361 agcggcgatg tgaagtttag ttaagttctt tagtatctgc atttacgact aatgaaaaaa
      421 acgcgtatgc ctttgccaga caagcgttat agttatcgcg gcaagtatcc caaagaagcc
      481 gataaaatag taatagagtg ttaatgcacc tgtaaaagcg caacgaaccg gactccacaa
      541 ctcttcacta tggcattttc ctgccagttc aaggagctta tttcgtccat gcgagataat
      601 gcctggccat aacacaatca ttaagcaact tattacccaa atgggccgtt cactcattaa
      661 tgccgcattc acgatgatta ccagggccaa ccctgtcaac gtcatcataa tgataaaacg
      721 gtgagaacta ggatcaacat taaataataa accggccagc atactgatta aaaacatcgc
      781 gcccgcagtg ctgaacacga tgataatggc atatacagga actaaacttg caacgacaat
      841 cgccaccaaa gagagtaaaa acagatatat tacgcccgcg atggcggcat ttatcttgtt
      901 gatatcctta cttaagccat acatacaaag taataataac cctatggcga catacattgg
      961 tggtgaatca tatttggttg ccgtaatttc gagatactta gctccccagg ctagtcctgc
     1021 ggttgccacc aaacctaaag cggtaatgag ccatataccg tttaatatcc tggcctcgtg
     1081 ctgaaaatct aatttattgc tgttttgcga gtatgattcc attcttttag ttgtccttag
     1141 aaaaaatatt aatattcacc atcaataaat ttaaaaccac gcatacaaaa tacttttgta
     1201 tttatggata cggaaaacga ttccgctcgc tcataaggca tattttatac ctggtacttg
     1261 ttaagtgtcg tttttagtaa gaaaaaaatc ccgacacatg gccgggatag tgatggtgaa
     1321 aaagaattaa cggcggttgc cgaagatccg taacaacatc aggaacaggt tgatgaagtc
     1381 cagatacaag gttaacgcgc caagaatgga atatttgcgc aggttcgacg tgtcgcgggt
     1441 atcaatctgc tcacccatat ttttcaattt ctgagtgtca tacgccgtca atccgacaaa
     1501 gacaatcacg ccgatgtagg taactgccca catcaatgct tcgcttttca gccagaagtt
     1561 gaccagcgat gccagcacaa tgccgattaa cgccataaac agcatattgc caaagccact
     1621 taaatcgcgc ttcgtggtgt aaccgtacag gctcattgcg ccgaacatcc cggcagtaac
     1681 gacgaaagta ctggcgatag aagcagcggt atagacaatg aatatactgg aaagcgtaag
     1741 acccgtcagc gccgaataaa gcataaagag catcgtcgtt acacctgcgc tcagcttttg
     1801 aatcatcgct gataacacaa taaccaatgc taattgcgcg atgatcagac cgattaaaaa
     1861 gacacggtta gtgaacaaca gctccatcac ggccgcggaa ttagccgcat accaggcaac
     1921 aaatgcggtc agcaacaagc caacggtcat ccagccatag acttgcgcca tataagtttg
     1981 caagccagcc cggggttgta cgattgaatc agaacgtggg aatctgtcca tgacgatctc
     2041 ctgaagatat aaggaatatc ttaaggatac tgcaaaatga tgaggctgtg catcgacgca
     2101 gcgtaaacgc atgtactgag cggtgaaatt gccggacgca gcggtgcctt atccggctaa
     2161 caaaaaacta ccagcgtttt gccgcctgca gatcgctctc ccgggcttca acccagcggt
     2221 cgccttccgg cgtggcttcg cgcttccaga acggtgcgcg ggttttgaga taatccataa
     2281 taaactgccc ggcttcaaac gcactgctgc gatgcgcact ggtgacaccg acaaaaacga
     2341 tttcatcgcc cggccataat tccccgatgc ggtgaatcac cgtgacgcgc cccagcggcc
     2401 agcggttacg cgcttcatca acaatttctg ccagtgcttt ttcagtcatc cccggatagt
     2461 gttcgagggt taatgctttg acgctgtcgc ccaggttatg gttgcgcacc tttccggtga
     2521 aggtgactac cgcaccgtct tcgtcacgct ccgccagcca cgggtactct tctcctacgc
     2581 tgaacggctg cgggccgaca acaattttgg tttctgccat cttaacctcc ggttaccggc
     2641 gggaagaaag ctacttcgtc gccgtcagtc agcggatggt caaaactcac cagcgtctgg
     2701 ttgacggcag ccagcaattt gccatcttcc agcgccagcg cccagcgatc gctctgcgca
     2761 gccaggtgct ggcgtaacgc ttcaacggtt gggtaatccg cagccacttc ggttgcatct
     2821 gctcccacca actcgcgcac ctgggcgaaa aaaagaactt taatcatccg cttccacctt
     2881 aaagtcaccc gacttgccgc cgctcttcgc cagcaaacgt accggaccaa tcaccatatc
     2941 tttttgcacc gctttgcaca tgtcataaat ggtcagcgcc gccacggagg ccgcggttaa
     3001 cgcttccatt tcgacaccgg ttttcccggt caggcggcat aaggtttcta tacgcacccg
     3061 attgtgctcc ggctcggcct gtaaattgac ttcaactttg ctgagcatca gcgggtgaca
     3121 cagcgggatc agatcccaag tgcgttttgc cgcctgaata ccggcaatac gcgcagtggc
     3181 aaatacgtcg cctttgtggt ggcgaccatc aataatcatc gccagcgtct cgctgcgcat
     3241 ggtgacaaag gcttccgccc gcgcttcacg cacggtttcc gctttggcgg agacatccac
     3301 catgtgcgct tcgccagcgg cgttgatatg ggtcagttgc gacatactta tttcttcaaa
     3361 tgtggatgga aattacacgg acgcgtacgg gcatccagct gcggcgcgat gatattttcc
     3421 catgcggtac ggcacgcttt ggtcgaaccc ggcatggcga aaatcagcgt tttgttagcg
     3481 acgcccgcta ccgcacgaga ttgcaacgtg gaagtgccaa tctcttcaaa cgacaacata
     3541 cggaacactt caccaaaacc ttcaaactca cggtcgaaca acggcagcag cgcttcggga
     3601 gcctgatcac cttccgtcag gccagtacca cccgtaatca ataccacttg tacatcgtcg
     3661 ctggcgatcc acgcagatac ctgagcgcga atagcgtagc ggttttcttt cacaatggct
     3721 ttatcgacaa cgtgatggcc cgcttcttgc gccgaatcgc gcagatagtg accggaggta
     3781 tcgtcttctt caccgcgacg attagaaacc gtaagaatag caatacgggt cgggataaat
     3841 tcagtgctta cctgactcat ctgatctctc cttttgacgt tttagccgcc aatgtacgat
     3901 aagttttgcg taataccggt gttgttttga tgcaggaaat gggtctgttt cttctcccgc
     3961 agcgccgctg aaatacgcgc ttccagcgcc tgttgctggg tatcgtcttc cagcagatcg
     4021 cgcaggttaa cgccgccttc accaaacagg cagagatgga gtttaccaat ggaggaaacg
     4081 cgcaggcggt tgcaagtggc gcagaagtct ttttcatacg gcatgataag gccaatctct
     4141 ccggcgtaat ctggatggca aaagacttgc gcgggaccgt cgctgcgttg acgtaattgg
     4201 tggatccagc cgcgacgcag tagctcgtca cgcagaacct gaccagagat gtgatgctta
     4261 cggaagagct cgctgccctc gcccgtttcc atcagttcga tgaaacgcag ctggataggg
     4321 cgatgctgga tccagttcag aaaggtgtcg agctggtgat gattaacatc acgcatcagc
     4381 acggtattga ctttgacctt ctcaaaaccg gcctcaaatg cagcatcaat ccctgccatg
     4441 acctggttga atttatcctg cccggtaata gcgtgaaact ggcgggcgtc caaactgtcg
     4501 acactgacgt taatgccagt aagtcccgca tcgcgccagt tcgccacatc gcgttccaga
     4561 cggtaaccat tggtggtgac cgcaatctgg cggatagcgt cgttttcccg cacagcggcg
     4621 atgatatcgg taaagtcgcg gcgtaaagac ggctctcctc ctgtcaggcg cactttttcg
     4681 gtgcccagac tggcgaaggc gcgcgtaacc cggcgaattt catcgacggt aagaaagcct
     4741 ttattggtga cgccgctcgg tttgtagcca tccggcaggc agtaggtgca acgaaagtta
     4801 cacacatcgg taatcgacag gcgcaagtag taaaacttac gcgcaaatgc atcagtcagt
     4861 tgtgaagcca tgtacacctt tccagatacg ggaggcgaag tcatttcttc cttcgccctg
     4921 gtggcaatct ttacgagcat tgtcacggcc aaagcaccgt atcagttgac ccaggtgcag
     4981 aggctagagt gtttagtggt taagccgata ctagcgtgta aatgagattt ttaccatcca
     5041 cactttcgct atgtaatcat gtatatagcg tcatgatcgt gctatttcgc tacagaataa
     5101 gggaaaatgg cggttttact ttgatataca tcatcaggga ggcatagcgt gcatcgtcct
     5161 ttagagatag aagaaaatag tttgtatcct caatattggc aggttaattg ctgtttcccc
     5221 gcaatttgcg ctactgtagc gcggataatc tgactccagg aaactatatg cgcaatcgta
     5281 cgctggctga tcttgatcgt gtcgttgctc tcggcggagg gcatggactg ggacgcgttc
     5341 tctcatcact ttcgtctttg ggttctcgtt taacgggtat cgtcaccacc acggataatg
     5401 gtggctcgac ggggcgtatt cgccgttcag aaggcggcat tgcttggggc gatatgcgca
     5461 actgcctcaa ccagctgata acggaaccga gcgtcgcctc cacgatgttt gaataccgtt
     5521 ttggcggcaa tggcgaactt tccggtcata atctcggaaa cttgatgtta aaggcgttgg
     5581 atcaccttag cgtgcggcct ctggaagcca tcaatttaat tcgtaatctg ctgaaagtgg
     5641 atacgcattt gattccaatg tcagagcatc ctgttgatct gatggcgatt gacgatcagg
     5701 ggcatgaagt ttacggcgag gtcaatatcg accagttaac tacgccgatt caagagttat
     5761 tgttaacgcc taatgtaccc gcaacgcgtg aggcggttca cgctatcaat gaagcggatc
     5821 tcatcattat tgggcctggc agtttttata ccagcctgat gccaattctg ctgctgaagg
     5881 aaatcgccca ggcattacgc cgcacgccag cgccgatggt ttatatcggc aatctggggc
     5941 gtgagttgag tttacctgcg gctaatttga agctggaaag caagctggca attatggagc
     6001 agtatgttgg taaaaaggtc attgatgcgg tcatcgtcgg gccaaaagtg gatgtctcgg
     6061 cggtgaaaga gcggattgtg atccaggagg tactggaggc cagcgatatt ccgtatcgtc
     6121 atgaccgcca gttgttacat aacgcgctgg aaaaggcgtt acaggcttta ggttaacgga
     6181 aatccgaagg aaaattccgg cttcctattg aagacaaagt gcgcgttgtt tatgccggat
     6241 gcggcgtgaa cgccttatcc ggcctacaaa ccgcgcaaat tcaatatatt gcggagaaaa
     6301 tgtaggcctg ataagcgtag cgcatcaggc tgttttccgt ttgtcatcag tcttcttcgc
     6361 tatcctgtta cgatgcggca ataaacaact cacgcaactg atgcaactgg tcacgaattt
     6421 gtgccgcttc ttcgaactcc agattctgcg cgtgttgcat catcagccct tccagttcat
     6481 ggattttctg ctgcaacgct ttcggcgaca tatccatcgg cacattatcc ggctcaacaa
     6541 tcgggcgcga ttttcctctg cccttcgctt tggttttggc aatgttctgc cccagcgcca
     6601 ggatatcgac cactttcttg ttcaagcctt gcggcgtaat gccgtgttcc tcgttgtact
     6661 tctgctgttt ctcacggcga cgttcggttt cgccaatcgc tttcgccatt gatggagtga
     6721 tcttatcgcc gtagagaatc gctttaccgt taacgttacg tgccgcacga ccaatggtct
     6781 ggatcaacga acgttcggaa cgcaggaagc cttctttgtc agcgtcgagg atcgccacca
     6841 gcgacacttc cggcatatcc agaccttcac gcagtaagtt gatccctacc aatacgtcga
     6901 actcacccag acgcaagtcg cggataatct ccatacgctc gacggtgtcg atatctgagt
     6961 gaagataacg cacgcgctca ccgtgttctt cgagatattc ggtaagatct tccgccattc
     7021 gcttggtcag tgtggtgacc agcacgcgtt cgttaattgc cgctcgctga cgaatctccg
     7081 aaagaagatc atcaacctgt gtcgccaccg gccgcacttc gataatcggg tcaagcaatc
     7141 cggttggacg caccacctga tccaccacat cgccgccgga tttttccagc tcgtaattac
     7201 ccggcgtcgc cgaaacatag atggtttgcg gcgctaatgc ttcgaactct tcaaacttaa
     7261 gcggacggtt atccagcgct gatggcaggc ggaagccgta ctccaccagt gtctctttac
     7321 gcgcccggtc accgcgatac atgccgccaa tttgtggaat ggtgacgtga gattcatcga
     7381 cgaccagcag cccatcggca ggcaggtaat caaacagcgt cggcggtggc tcacccggtc
     7441 cacgaccgga gaggaagcgc gagtagtttt caatccccga acagtagccc agctcgttca
     7501 tcatctccag atcaaactgg gtacgctggg tcagccgctg ctcttccagc agtttgttgt
     7561 tttccaacag cactttgcgt ctggcggcca gttcttcttt gatctcctcc atcgcctgta
     7621 cgatgcgctc gcgcggtgtg acgtagtgcg ttttcgggta gatggtaaaa cgtggaatag
     7681 tggaaacaat ctgcccggtc agcgggtcaa ataacgacaa tcgttccact tcctcgtcaa
     7741 acagttccac gcgaagtgca atgtcatccg attctgccgg gaagatatct atcacctcgc
     7801 cacgaacgcg gaaagtacca cgctggaatg cttgatcatt acgagcgtat tgcagctccg
     7861 ccagtcggcg cagaatcgcg cgctgatcga taatcatacc gaccgtgaga tggagcatca
     7921 tcttgagata taaatcagga tcgcccagac cataaatcgc ggaaacagac gccactacaa
     7981 tcacatcacg ccgctccagc atcgctttgg tggcggacaa acgcatctgc tcaatatgtt
     8041 cgttaaccga ggcatctttc tcaatgaagg tgtcggaact cggtacatag gcttccggct
     8101 gatagtagtc gtagtaggag acgaaatact ccaccgcgtt ttccgggaag aactctttca
     8161 tttcgccata cagctgggcc gccagcgttt tgttgggcgc aagtaccatg gttgggcgct
     8221 gaaggtcagc aatgacattg gcaatggtga aggttttccc ggagccagtc acgccaagta
     8281 acgtctggtg cgccaggcca tcttccagcc cctcttcgag acgtcgaatc gcctctggct
     8341 gatcgccaga aggtttaaaa gcggaattca gtttgaacgg tttactcatg agtcgctacc
     8401 tgaaggagtt gggcgggcag gtatgtaatt ttactcgtcg tacttaatta tgccaacaaa
     8461 ttatactgga taaaaaaaca gttcatcacc ataatatttc tgatacagcg taaactccgc
     8521 tgtcaatcat gagcaaaatt tactctgtgg cgagataaaa ctccggcctt accgggttat
     8581 ccccaaagca acggcttttt taacatttgt caagatgagt gatgacagtt ttatggcaag
     8641 agatgcctgt tcagtgactg ccattgcatt tatctaacca gttaaaaaat aaaagatatt
     8701 tctttgagcc gtctttaacg ccaattcgcg tgcaagccgc gtattctctc gcttgcctcg
     8761 tgttttctaa ctctaataca catggttatc cacaggaata gtggataact gtctccagcc
     8821 cctatccccc gccgcctggg caattcccac attcgccgca tgaggcggaa gtaaaatttt
     8881 tttcgtgatt attttagaat ttaattggtt aaattgcagt caatcgaaga cgcgatctcg
     8941 ctcacaattt aaccaaatac agaatggcta caacaaggca aggtttatat actttccggt
     9001 tgccgcattt tctggatttt ctgcaagcca ggggagctct cccagcagcg gcgcgggaat
     9061 catgcgggtg agcgtggtca tatattcagc gtgacgtttt cccggaggcg taacatcgtt
     9121 cgccacccaa cccgccagag tcagtccggc gtgttgtatt gcctgcacag tcaacatcgc
     9181 gtgattaata cagccgagtt tcacaccaac taccagtatc accggcagtt gttcctgtgt
     9241 tacccaatct gcaaaagtga aagtgtcaga aagcggcgta aaccagccgc cagcaccttc
     9301 cactaacacc cagtcagcct gttgttcaag cgcgcgtaat ccggcgctca ttaccaatga
     9361 ttctatcggc ctgccctctt gggcgctgat gatgtgcggc gaggtgggtt ctgcgaaggt
     9421 gtaaggattt accgttgcgt aatccagctg caggctgctg ttgtgctgta acgccagcgc
     9481 gtcactatta cgtaagcctt ccggggtctt ttcgctgcca gaggcgaccg gtttataacc
     9541 tgccgtccga taacctactg ccttcgcggc ttgtaaaagt gcacaactgg cgacggtttt
     9601 ccccacttcg gtatccgttc cggtgacaaa ataacgttta ctcacgagca atcactccca
     9661 aaaaaagatg atacgtcaga ggatatcgcc cctgttgttg cggccaggcc agttgcaatc
     9721 gctgcaactg cgaacgcgtt aatattcgcg ggtcgcgccc ttcatgaaga tgcgtagcac
     9781 cgatgccttt cagcgaacgc atggcactga gcgcatcatc aaaccatagt gtgatgagct
     9841 gaatatgatg ttgataacgc acgccgttca gcgactcttc gatttcatct ggcggtaaaa
     9901 agcgattagc atgcggacgc tcgtccaccg cctgccgcgc ctgatgcagt tcgggtaacg
     9961 atccctgcac cagcgtggta aacgcgacca cgcctccggg gcgcaccacc cgatacagct
    10021 cgcggagtgc cgtggataaa ttaccgcacc actgcaccgc gagattgctc catgcaagat
    10081 cgaacgtcgc agtcgctaac ggcagggatt cgatatctcc cgccagataa tgat
//
LOCUS       DBKEKI010000143        10103 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.144, whole genome shotgun sequence.
ACCESSION   DBKEKI010000143 DBKEKI010000000
VERSION     DBKEKI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.144"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(77..1360)
                     /gene="aroA"
                     /locus_tag="ACUQPX_003493"
     CDS             complement(77..1360)
                     /gene="aroA"
                     /locus_tag="ACUQPX_003493"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HHV9058070.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1431..2519)
                     /gene="serC"
                     /locus_tag="ACUQPX_003494"
     CDS             complement(1431..2519)
                     /gene="serC"
                     /locus_tag="ACUQPX_003494"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HHV9058071.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2718..3345)
                     /locus_tag="ACUQPX_003495"
                     /pseudo
     CDS             complement(2718..3345)
                     /locus_tag="ACUQPX_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3475..5235
                     /gene="ycaO"
                     /locus_tag="ACUQPX_003496"
     CDS             3475..5235
                     /gene="ycaO"
                     /locus_tag="ACUQPX_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HHV9058072.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYGKLQRAKAAFWAK"
     gene            5641..6498
                     /gene="focA"
                     /locus_tag="ACUQPX_003497"
     CDS             5641..6498
                     /gene="focA"
                     /locus_tag="ACUQPX_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HHV9058073.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6553..8835
                     /gene="pflB"
                     /locus_tag="ACUQPX_003498"
     CDS             6553..8835
                     /gene="pflB"
                     /locus_tag="ACUQPX_003498"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HHV9058074.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRNFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9027..9767
                     /gene="pflA"
                     /locus_tag="ACUQPX_003499"
     CDS             9027..9767
                     /gene="pflA"
                     /locus_tag="ACUQPX_003499"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HHV9058075.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9849..>10103)
                     /locus_tag="ACUQPX_003500"
     CDS             complement(9849..>10103)
                     /locus_tag="ACUQPX_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HHV9058076.1"
                     /translation="GDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYL
                     GIEDADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT         2593 a         2535 c         2395 g         2580 t
ORIGIN      
        1 ttaactgatc ggcattacga caatgtgccg acgttttgat aaagaaagat ttggctattt
       61 attgcccgtt gttcattcag gctgcctggc taatccgcgc cagctgctcg aaataatccg
      121 gaaatgtttt ggccgtgcat ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca
      181 ccagcgagaa acacatcgcc atccggtgat cattgtatgt cgcgatctcg gcaaagttca
      241 gtttttccgg aggagtgata cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt
      301 tacgcagttc tgttgccatc gcaaacaggc gatcggtctc tttaacacgc cagttataga
      361 tattgcgcag cgtggtggtg ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg
      421 catcaggaat atggttcata tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa
      481 tataatcatc gccccagcaa atggtcgcgc ccattttttc cagcacatca gcaaagcgaa
      541 tatcaccctg catactgtta cgtccaatac cggtcacttt tacagtgccg cctttgattg
      601 ctgctgctgc caggaagtaa gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg
      661 gagactgata agactgcccg ccttttacga caaattgttg atagtgctga ttttcaattt
      721 caacaccaaa cgtcttcatc agattgagtg tgatgtcgat ataaggttta gaaaccagat
      781 cgcctttaat acgaatcacc gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg
      841 tgaggaattg gctggaaacg gagccatcaa cgtcaacgtt gccgccagta aagccgccct
      901 gtaaacgcaa cggcggataa ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc
      961 gcagcgcatc caccagatga ccaatcgggc gctctttcat acgcggctca ccggtcagca
     1021 caatatcatt gctatccaga caaagagctg ccgccagcgg acgcattgcc gttccggcgt
     1081 taccgaggaa caactccagg gcaccttctg cgtgtaatgg accgccgtta ccgataattt
     1141 cgcaacgcgt acgatcggct gaaagcgtat agcttaaccc taacgctgtt aatgcattca
     1201 gcatatggcg cacgtcgtcg ctatccagca gattggttaa tactgttttg ccgtgtgcta
     1261 atgccgcaag caataaagcg cggttagaaa cgctcttgga accgggcaga ttaatagtgc
     1321 catcgacacg agcgatgggt tgtaacgtca gggattccat gaactcaact ctctacaaca
     1381 gaaataaaaa ccccacagac tggctgtggg gattaagcaa aatttcggca ttaaccgtga
     1441 cggcgttcga actcaaccat gaagtctgtc agcgctttaa cgccttccag cggcatggca
     1501 ttataaatag acgcgcgcat tccgccgacc acacggtgtc ctttcagtgc atgaaggcct
     1561 gcagcaaaag actcttcaag gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc
     1621 acgttcatcc gcgaacggtt agctttcgcc acgtcattgc ggtagaaatc gctgttatca
     1681 atcaccccat atagcagttc tgctttttgc tgattgattt tatccatttc agctacaccg
     1741 ccgttcgctt tcagccattt aaagaccaga ccagatagat accaggcaaa tgtcggcggc
     1801 gtgttaaaca tggagccgtt atcgttgagg atggaataat ccagaatcga cggacacgcg
     1861 atattcgctt tgcccagcaa atcttcacga acgatgacga ttgtcaggcc agccgggccg
     1921 atatttttct gcgcgccagc gtaaattaca ccataacggc tgacgtcaat cggacgggaa
     1981 agaatggttg aagagaagtc ggcggcgacc accacatctt tgccgaagtc tggcgtctcg
     2041 tcgatggcga taccgtcgat ggtttcattc gggcagtaat gcatataagc cgcattatca
     2101 gagagttgcc attcacgcat tggcttaacc gcgcgcagac catcaacagt cactttggcg
     2161 tcaaagacat taggcgtgca gtattttttc gcttctttaa tggcacttgc cgcccagtaa
     2221 ccggcatcaa cataatctgc ggtggtttta tcaccgagaa tattcagcgg taccgcagca
     2281 aactgaccgc gaccaccgcc atggcagaat aataccttgt agttggaggg gacattaaga
     2341 agatcgcgaa aatccttctc ggcttcctct gcaacctgaa tgaactcttt gccacggtga
     2401 ctcacttcca tcaccgacgt accaagaccg ttccagtcgc gcagttcctg ttgagcctgt
     2461 ttaagcacct ctgccggtag cattgccgga ccagaactaa aattgaagat ttgagccatt
     2521 tcccctcacc acgttgcgtt gctattaacc cgcggtcaat cgattgcgac cacaggtaat
     2581 acattattcc tgtggctatc ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac
     2641 cgttgagggc aaaaatgtgg cttgcatcac acaaaagaat ctctcggatt attagcaaaa
     2701 agccgatgtt tttactgtca ggtcacccgt tttgcccgac ttgccttcgt ccattctgga
     2761 tttgcacagc gcggacataa ttgtttttcc cccgcgttca tatgaatgat ttgactacaa
     2821 cgaatgcagg catagtccgc cgactccggc accactttgt aacgctgcgt acaatcactg
     2881 ggtaaaataa gtaaacccgg tttcaccttg tcatcttcaa agaaatagac actgatttgc
     2941 ccgttggttt cgagaatcgc cagacgtacc tgccccagct gctccacgcc acgcaatcgt
     3001 agctccataa agaactcaaa ttccgtcatg ttggagttat tgagtttcga ccaggccagc
     3061 tcgccatcac attaccaggc ggtataacaa cgccagagtg ataaagacga taagtaccgg
     3121 caccatcggc acatcatcat aaaacgccac atctcccgcc gctgaaccca gcgtgagaat
     3181 gattaacact tcaaacagcg acatctgtcg cacaccgcgt cttccggtca ttttgaggaa
     3241 caaaaaaacc agtacaaagg tataaagact acgtagtgca acttcgccaa ggaaatcaaa
     3301 aggcacttta tcaaatgcca tacggtggag atcgaacgcc ttcattttta tgctcttaac
     3361 gtggtttatc cgctaaaagc atagtcaaca gttcatcggc atggcggcgg tgatagcgcc
     3421 tttgagataa aacccgtatt attgcgcgct ttccgtacga ctaaagtgat tttcatgacg
     3481 caaacattta tccccggcaa agatgccgct ctggaagatt ccatcgctcg cttccagcaa
     3541 aaactttcag acctcggctt tcagattgaa gaggcctcct ggctgaatcc cgtgcctaac
     3601 gtctggtctg tacatattcg cgacaaagag tgcgcactgt gttttaccaa cggtaaaggc
     3661 gcaaccaaga aagcggcgct ggcttctgca ctcggtgaat atttcgagcg tctctcaacc
     3721 aactactttt ttgctgattt ctggctgggc gaaaccatcg ccaacggtcc atttgtgcat
     3781 tatcccaacg aaaaatggtt cccactgagc gaaaatgacg atgtgccaga agggctgctc
     3841 gatgaccgtc tgcgcgcatt ttacgatccg gagaatgaat tgaccggcag tatgctgatt
     3901 gacctacaat ccggtaacga agatcgtggt atttgcggtc tgccgtttac gcgccagtcc
     3961 gacaatcaga ccgtttatat tccgatgaat atcattggta acctgtacgt ttctaacggt
     4021 atgtccgctg gcaatacccg caacgaagca cgcgttcagg ggttgtccga agttttcgaa
     4081 cgctacgtga aaaaccgcat tattgctgaa agcatcagct tgccggaaat cccggcagac
     4141 gtgctggcgc gttacccagc ggtggttgaa gcgatcgaaa cactggaaac agaaggtttc
     4201 ccgatcttcg catatgatgg ttcccttggc ggccagtatc cggtgatttg cgtggtactg
     4261 tttaatcctg caaacggcac ctgctttgcc tctttcggtg cgcatcctga ttttggcgta
     4321 gcactggaac gtaccgtgac cgagctgctg caaggtcgtg gcctgaaaga tttggatgtg
     4381 tttactccgc caaccttcga tgatgaagaa gtcgctgaac ataccaacct cgaaacgcac
     4441 tttatcgatt ccagcggttt aatctcctgg gacctgttca agcaggatgc cgattatccg
     4501 tttgtggact ggaatttctc cggcaccacg gaagaagagt ttgctacgct gatggctatc
     4561 ttcaacaaag aagataaaga agtttatatt gccgattacg agcatctggg cgtttatgct
     4621 tgccgtatta tcgtgcctgg catgtccgat atttatccgg ctgaagatct gtggctcgcg
     4681 aataacagta tgggcagcca tttacgtgaa acgattcttt cgctaccagg cagcgagtgg
     4741 gaaaaagaag attacctgaa cctcatcgag caactggatg aagaaggttt tgatgacttt
     4801 acccgcgtgc gtgagctgtt gggtctggcg accgggtcgg ataacggttg gtacaccctg
     4861 cgtatcggtg aattaaaagc catgctggcg ctggctggtg gcgatctgga acaggctctg
     4921 gtctggaccg aatggacgat ggagtttaac tcatcagtat ttagtccgga acgcgccaac
     4981 tattatcgct gcctgcaaac gttgttatta ctggcacagg aagaagatcg ccagccgctg
     5041 caatatctga atgcgtttgt tcgcatgtac ggcgcagatg ccgtagaagc cgccagtgcg
     5101 gcaatgagcg gcgaagcggc gttttacggc ctgcaaccgg tagatagcga tctgcacgcg
     5161 tttgctgcac atcagtcgtt gttgaaggcc tacggaaagc tgcagcgcgc caaagcagca
     5221 ttctgggcaa aataaaatca aatagcctac gcaatgtagg cttaatgatt agtctgagtt
     5281 atattacggg gcgttttttt aatgccccgc tttacatata tttgcattaa taaaataatt
     5341 gtaattataa ggttaaatat cggtaatttg tatttaataa atacgatcga tattgttact
     5401 ttattcgcct gatgctccct tttaattaac tgttttagcg gaggatgcgg aaaaaattca
     5461 actcatttgt taatttttaa aatttatttt tatttggata atcaaatatt tactccgtat
     5521 ttgcataaag accatgcgag ttacgggcct ataagccagg cgagatatga tctatatcaa
     5581 tttctcatct ataatgcttt gttagtatct cgtcgccgac ttaataaaga gagagttagt
     5641 gtgaaagctg acaacccttt tgatctttta cttcctgctg caatggccaa agtggccgaa
     5701 gaggcgggtg tctataaagc aacgaaacat ccgcttaaga ctttctatct ggcgattacc
     5761 gccggtgttt tcatctcaat cgcattcgtc ttctatatca cagcaaccac tggcacaggc
     5821 acaatgccct tcggcatggc aaaactggtt ggcggcattt gcttctctct ggggctgatt
     5881 ctttgtgttg tctgcggagc cgatctcttt acttccacag tgttgattgt tgttgctaag
     5941 gcgagtgggc gcatcacctg gggtcagttg gctaaaaact ggctaaatgt ctattttggc
     6001 aacctagtcg gcgcactgct gtttgtactt ttaatgtggc tttccggcga gtatatgacc
     6061 gcaaatggtc aatggggact aaacgtccta caaaccgccg accacaaagt gcaccatact
     6121 tttattgagg ccgtctgtct tggtatcctg gcaaacctga tggtatgtct ggcagtatgg
     6181 atgagttatt ctggccgcag cctgatggac aaagcgttca ttatggtgct gccggtcgcg
     6241 atgtttgttg ccagcggttt tgagcacagt atcgcaaaca tgtttatgat cccgatgggt
     6301 attgtaatcc gcgacttcgc atctccggaa ttctggaccg ctgtcggttc tgcaccggaa
     6361 aatttttctc acctgaccgt gatgaacttc atcactgata acctgatccc ggttacgatc
     6421 ggtaatatta tcggcggtgg tttgttggtt gggttgacat actgggtcat ttacctgcgt
     6481 gaaaacgatc accattaatg gttgtcgaag tacgcagtaa ataaaaaatc cacttaagaa
     6541 ggtaggtgtt acatgtccga gcttaatgaa aagttagcca cagcctggga aggttttacc
     6601 aaaggtgact ggcagaatga agtaaacgtc cgtaacttca ttcagaaaaa ctacactccg
     6661 tacgagggtg acgagtcctt cctggctggc gctactgaag cgaccaccac cctgtgggac
     6721 aaagtaatgg aaggcgttaa actggaaaac cgcactcacg cgccagttga ttttgacacc
     6781 gctgttgctt ccaccatcac ctctcacgac gctggctaca tcaataaagc gttggaaaaa
     6841 gttgttggtc tgcagactga agctccgctg aaacgtgctc ttatcccgtt cggtggtatc
     6901 aaaatgatcg aaggttcctg caaagcgtac aaccgcgaac tggacccgat gatcaaaaaa
     6961 atcttcactg aataccgtaa aactcacaac cagggcgtgt tcgacgttta cactccggac
     7021 atcctgcgtt gccgtaaatc cggtgttctg accggtctgc cagatgctta tggccgtggc
     7081 cgtatcatcg gtgactaccg tcgcgttgcg ctgtacggta tcgactacct gatgaaagac
     7141 aaatacgctc agttcacctc tctgcaggct gatctggaaa acggcgtaaa cctggaacag
     7201 actatccgtc tgcgcgaaga aatcgctgaa cagcaccgcg ctctgggtca gatgaaagaa
     7261 atggctgcga aatacggcta cgacatctct ggtccggcta ccaacgctca ggaagctatc
     7321 cagtggactt acttcggcta cctggctgct gttaagtctc agaacggtgc tgcaatgtcc
     7381 ttcggtcgta cctccacctt cctggatgtg tacatcgaac gtgacctgaa agctggcaag
     7441 atcaccgaac aagaagcgca ggaaatggtt gaccacctgg tcatgaaact gcgtatggtt
     7501 cgcttcctgc gtactccgga atacgatgaa ctgttctctg gcgacccaat ctgggcaacc
     7561 gaatctatcg gtggtatggg cctcgatggt cgtaccctgg ttaccaaaaa cagcttccgt
     7621 ttcctgaaca ccctgtacac tatggggccg tctccggaac cgaacatgac cattctgtgg
     7681 tctgaaaaac tgccgctgaa cttcaagaaa ttcgccgcta aagtgtccat cgacacctct
     7741 tctctgcagt atgagaacga tgacctgatg cgtccggact tcaacaacga tgactacgct
     7801 atcgcttgct gcgtaagccc gatgatcgtt ggtaaacaaa tgcagttctt cggtgcgcgt
     7861 gcaaacctgg cgaaaaccat gctgtacgca atcaacggcg gcgttgacga aaaactgaaa
     7921 atgcaggttg gtccgaagtc tgaaccgatc aaaggcgatc tcctgagcta cgatgaagtg
     7981 atggagcgca tggatcactt catggactgg ctggctaaac agtacatcac tgcactgaac
     8041 atcatccact acatgcacga caagtacagc tacgaagcct ctctgatggc gctgcacgac
     8101 cgtgacgtta tccgcaccat ggcgtgtggt atcgctggtc tgtccgttgc tgctgactcc
     8161 ctgtctgcaa tcaaatatgc gaaagttaaa ccgattcgtg acgaagacgg tctggctatc
     8221 gacttcgaaa tcgaaggcga atacccgcag tttggtaaca atgatccgcg tgtagatgac
     8281 ctggctgttg acctggtaga acgtttcatg aagaaaattc agaaactgca cacctaccgt
     8341 gacgctatcc cgactcagtc tgttctgacc atcacttcta acgttgtgta tggtaagaaa
     8401 actggtaaca ccccagacgg tcgtcgtgct ggcgcgccgt tcggaccggg tgctaacccg
     8461 atgcacggtc gtgaccagaa aggtgctgta gcgtctctga cttccgttgc taaactgccg
     8521 tttgcttacg ctaaagatgg tatctcctac accttctcta tcgttccgaa cgcactgggt
     8581 aaagacgacg aagttcgtaa gaccaacctg gctggtctga tggatggtta cttccaccac
     8641 gaagcatcca tcgaaggtgg tcagcacctg aacgttaacg tgatgaaccg tgaaatgctg
     8701 ctcgacgcga tggaaaaccc ggaaaaatat ccgcagctga ccatccgtgt atctggctac
     8761 gcagtacgtt tcaactcgct gactaaagaa cagcagcagg acgttattac tcgtaccttc
     8821 actcaatcta tgtaattaga tttgactgaa atcgtacagt aaaaagcgta caataaaggc
     8881 tccacgaaag tggggccttt tttagcacga gagccttttt tgtcagctat ctatacttta
     8941 aggtgactgc caaaacagac tcgacgtagc cttcgagctg cgcaccaaca cggcctcaga
     9001 tgggccacat ctggagaaac accgcaatgt cagttattgg tcgcattcac tcctttgaat
     9061 cctgtggaac cgtagacggc ccaggtattc gctttatcac ctttttccag ggctgcctga
     9121 tgcgctgcct gtattgtcat aaccgcgaca cctgggacac gcatggcggt aaagaagtta
     9181 ccgttgaaga tttgatgaag gaagtggtga cctatcgcca ctttatgaac gcttccggcg
     9241 gcggcgttac cgcatccggc ggtgaagcaa tcctgcaagc tgagtttgtt cgtgactggt
     9301 tccgcgcctg taaaaaagaa ggcattcata cctgtctgga taccaacggt tttgttcgtc
     9361 gttacgatcc ggtgattgat gaactgctgg aagtaaccga cctggtaatg ctcgatctca
     9421 aacagatgaa cgacgagatc caccaaaatc tggttggagt ttccaaccac cgcacgctgg
     9481 agttcgctaa atatctggcg aacaaaaatg tgaaggtgtg gatccgctac gttgttgtcc
     9541 caggctggtc tgacgatgac gattcggcgc atcgcctcgg tgaatttacc cgtgatatgg
     9601 gtaacgttga gaaaatcgaa cttctcccct accacgaact gggcaaacac aaatgggtgg
     9661 cgatgggtga agaatacaaa ctcgatggtg ttaaaccacc gaagaaagag accatggaac
     9721 gcgtgaaagg cattcttgag cagtacggtc ataaggtaat gttctaattt ccccgtaaag
     9781 cggcaacttt attgagttgc cgctttttta ttccgcgctt cattctgcta cagcgttttt
     9841 aacacatctt atagtgcatc taccatatcc cgtacctgag ataataacga ctgataatag
     9901 ctcgcgtgaa gttcttcccc atcgaatacc actgtattac acaagaaagt gacatcggca
     9961 tcttcaatac caagataact acacatattt tttaaataat cgcttatatt tttttcccag
    10021 cccatctgga caaatgattc tttgtctcct ccaaccagcg ccacccaacg aactttattg
    10081 aatggtaatt tgtgcccatc tcc
//
LOCUS       DBKEKI010000144         9936 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.145, whole genome shotgun sequence.
ACCESSION   DBKEKI010000144 DBKEKI010000000
VERSION     DBKEKI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.145"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..443
                     /locus_tag="ACUQPX_003501"
     CDS             <1..443
                     /locus_tag="ACUQPX_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="member of the major facilitator superfamily (MFS)
                     of transporters; unknown function; may be associated with
                     transport or processing of arabinose polymers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HHV9058077.1"
                     /translation="VLGNMLSGRISGRYSPLRIAAVTDFIIVLALLMLFFCGGMKTTS
                     LIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNLGSAVGAYCGGMMLTL
                     GLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPLG"
     gene            complement(586..1494)
                     /gene="mak"
                     /locus_tag="ACUQPX_003502"
     CDS             complement(586..1494)
                     /gene="mak"
                     /locus_tag="ACUQPX_003502"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HHV9058078.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGILGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAVGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            1619..2530
                     /gene="rdgC"
                     /locus_tag="ACUQPX_003503"
     CDS             1619..2530
                     /gene="rdgC"
                     /locus_tag="ACUQPX_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HHV9058079.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(2608..3063)
                     /locus_tag="ACUQPX_003504"
     CDS             complement(2608..3063)
                     /locus_tag="ACUQPX_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SF0329 family protein"
                     /protein_id="HHV9058080.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(3060..3395)
                     /locus_tag="ACUQPX_003505"
                     /pseudo
     CDS             complement(3060..3395)
                     /locus_tag="ACUQPX_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(3550..3834)
                     /gene="ppnP"
                     /locus_tag="ACUQPX_003506"
     CDS             complement(3550..3834)
                     /gene="ppnP"
                     /locus_tag="ACUQPX_003506"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HHV9058081.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(3906..4583)
                     /locus_tag="ACUQPX_003507"
     CDS             complement(3906..4583)
                     /locus_tag="ACUQPX_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HHV9058082.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIYAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(4841..5032)
                     /gene="yaiA"
                     /locus_tag="ACUQPX_003508"
     CDS             complement(4841..5032)
                     /gene="yaiA"
                     /locus_tag="ACUQPX_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HHV9058083.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(5082..5606)
                     /gene="aroL"
                     /locus_tag="ACUQPX_003509"
     CDS             complement(5082..5606)
                     /gene="aroL"
                     /locus_tag="ACUQPX_003509"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HHV9058084.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(5789..6247)
                     /locus_tag="ACUQPX_003510"
     CDS             complement(5789..6247)
                     /locus_tag="ACUQPX_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HHV9058085.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            6367..7176
                     /gene="proC"
                     /locus_tag="ACUQPX_003511"
     CDS             6367..7176
                     /gene="proC"
                     /locus_tag="ACUQPX_003511"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HHV9058086.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(7193..8308)
                     /gene="adrA"
                     /locus_tag="ACUQPX_003512"
                     /gene_synonym="dgcC"
     CDS             complement(7193..8308)
                     /gene="adrA"
                     /locus_tag="ACUQPX_003512"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HHV9058087.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(8410..8730)
                     /gene="psiF"
                     /locus_tag="ACUQPX_003513"
     CDS             complement(8410..8730)
                     /gene="psiF"
                     /locus_tag="ACUQPX_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HHV9058088.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(8849..9936)
                     /gene="phoA"
                     /locus_tag="ACUQPX_003514"
                     /pseudo
     CDS             complement(8849..9936)
                     /gene="phoA"
                     /locus_tag="ACUQPX_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase"
BASE COUNT         2401 a         2637 c         2407 g         2491 t
ORIGIN      
        1 tggtgctggg aaatatgcta agtggcagga tttcaggacg ttattcacca ctgcgcattg
       61 cagcagtgac tgactttatc attgtactgg cactgctgat gctctttttc tgcggcggca
      121 tgaaaacaac gtcgcttatt tttgctttta tttgttgcgc gggattattt gccctttcag
      181 cacctctgca aatattgtta ctgcaaaatg ccaaaggcgg agagttatta ggtgccgcag
      241 gtgggcaaat agcgtttaac ctcggtagcg ccgtcggcgc atattgcgga ggtatgatgc
      301 tgacgctggg gctggcatat aattacgtgg cgctgcctgc cgccctgctt tcgtttgctg
      361 cgatgtcatc gttgctgctg tatggtcgct ataagcgcca gcaagcggcg gatagtccgg
      421 tgctggcgaa accactgggg tagattactg tcacggtgcg cgttgcttat tgccggatgc
      481 ggcgtgaacg ccttatccgc cctacgcggt tctggcacat tttgtaggcc tgataagacg
      541 cggcaagcgt cgcatcaggc atgatgcgcc aattgcctac gttttttact cttgtggcca
      601 taaccacgca gcgccgcgta cgccgctgga atcaccgtgc ttcgccttac gcaccggcgt
      661 ttcacattcg ccgccgaaga caaattgttt aatcaactgc ccaaccgttt gatataaacg
      721 gtctacattg ctcatcccgc cccccaggac aatcacatcc ggatcgagaa tattcacgac
      781 atgtgccagc gattttgcca gccgcagctc gtagcgacgc aatgccagtt ccgctaccgg
      841 atcgctttct tcaaccaggc ggataatttc actgcctttc agcgcatgtc cgctcaaacg
      901 acgataatcc gtcgcgaatc ccgtgcccga aataaaggtt tcaatacaac cttgtttacc
      961 gcaataacaa gggacttcct cgcgataacg cagttcgtct tcgtccatcc acggtagcgg
     1021 attgtgtccc cactcacctg ccgtgccatt gccgccgata tgcgtccgcc cattgaatgc
     1081 cacgcccgcg ccgcatcccg tgccgataat cacggcaaat accgtctgcg ctcccactgc
     1141 cgcgccatct actgcttctg aaaccgccag acagttggca tcatttgcca gccgcacttc
     1201 ccgctgcaac ctcgcgctta agtctttatc gaatggctga ccgttgagcc aggttgaatt
     1261 ggcattcttc accacaccgg tgtagggcga aatcgaacct agaatgccca tacctaccgt
     1321 tccgcgctgc cctgtcgcct gctccgccat atcaaccaac gtggcgatcg tttcaatagt
     1381 ctgccggtaa tcatcacgcg gcgtgggcag acgatggcgg tacaactgct cccctgcatc
     1441 gcccagtgca atcacttcag ttttggtgcc gcctaaatcg atacctatac gcactgtact
     1501 ctccttattt ttttcaatat caatagcgta gagacggaca accggattgg caatgcaagg
     1561 ccgccgacaa ttcgttatca tgcccgctaa atttaacgac aaggccgtgg aaattatcat
     1621 gctgtggttc aaaaatttaa tggtttaccg tcttagccgc gagatttcgc tgcgtgcaga
     1681 agagatggaa aaacagctag cctcgatggc atttacccca tgcggcagcc aggacatggc
     1741 gaagatgggc tgggttcctc cgatgggatc gcacagcgat gcgttaacgc acgttgccaa
     1801 tggtcaaatt gttatctgcg cgcgcaaaga agaaaaaatc ctcccgtctc cggtgattaa
     1861 acaggcgctg gaagcgaaaa tcgccaaact ggaagcggaa caggcgcgta agctgaagaa
     1921 aaccgaaaaa gattcgctga aagacgaagt gctgcactct ctgctgccgc gtgctttcag
     1981 ccgttttagc cagacaatga tgtggctcga cacggttaac ggtttgatta tggtggactg
     2041 cgccagtgcc aaaaaagcgg aagatacgct ggcattactg cgtaaaagcc tggggtcgtt
     2101 accggttgta ccattgagca tggaaaaccc gattgaactg acgctgaccg aatgggttcg
     2161 ctccggtagt gcggcacagg gcttccagct gcttgatgaa gccgagctga aatcgttgct
     2221 ggaagatggc ggcgtgatcc gcgcgaagaa acaagatctg accagcgaag agatcaccaa
     2281 tcacattgaa gccggaaaag tggtgactaa actggcgctc gactggcagc agcgcattca
     2341 gtttgtgatg tgcgacgatg gttcgctcaa gcgtcttaag ttctgcgacg agctgcgcga
     2401 tcaaaacgaa gatatcgacc gtgaagattt cgcccagcgt tttgatgccg atttcatcct
     2461 gatgactggt gaactggcag cgttaattca aaacctgatt gaaggattag gtggcgaagc
     2521 acaacgttaa ttgctgattt tcctttaatg ccggatgcga cgcctgccgc gtcttatccg
     2581 gcgtacgaag ccacaccagg catataatta ttcgctacgg cgagcaataa tttttagcgc
     2641 agcaatatta tgcgttttac gccgtaactg atctaatcgc ctcatcccta ttcgcctgtc
     2701 agcagcacgc agaatattca ctaaccccca tttatcgctg gtaaattgtt ttgttaacag
     2761 ctccgctttt ggggtatcaa tatattcacg caataaatca gaaattgaac aaatatccgt
     2821 caggtaagga taccagcaag tttcaccgtg aaaattacgc actccagcgt tacctgcaat
     2881 aaagtctttc gggtaatccc agatcacgtt tttatccaac gtgatccaat aacgcggtaa
     2941 atcggtactt ccaccatttt ggctgcgcat gggataacgg gtacagtgaa tttgcaggtt
     3001 gatcgttggc gtcagtaagt catagatctc ccgctgtaac ttgctccatg gacgttgtgt
     3061 cattgttttt cctcaagcca gtgacgcgct tcactgcgaa ttttccgcgt tgccccggtt
     3121 ttcactaaat cacgtaactg cgaaagatcg ggttgttcga caaataagag taatgatgga
     3181 tcctctttta accacgccgt tttcacatcg gcagccagct gtggattatt aatagccagc
     3241 gaccgatcct gaggctcagt caatgtcgtt aacaacgacg ccggggtatg tgcgttgcgt
     3301 aatgccgttt ggcgcaaatg atcattgcga ctttgcagca atgcacgcca ccattcatca
     3361 tctgatttat cttttatcgt agccagttcc gcaaaaaata cctggagatc ttcactgata
     3421 tttatatcaa ccttgatgaa ttatgaatat atcggaaatt gaaaaaatcg cagcaaagga
     3481 aatattatta tgggttaaag agaggatatt tcagaaaaat gccctcccgt tcaaggggaa
     3541 ggcgaggaat tacagatagc ggcacagata agaggtgggt tcggcaactt gaagatgaaa
     3601 ctcactgtga ccgggaacat taaacaccga accggcttca tacacttgcc agtcggtcgc
     3661 gtcaggcagt aacacattca gcgcgccact gattaccgtc atctcttccg gctcagcggt
     3721 gctgaaggtg tattcgcctt caaccataac acccacgctg gcgcgaccag tgctgctgct
     3781 ggaaaagccg atcgatttca ctttgccgga aaagtactca ttactttgaa gcataaactg
     3841 gccctgtctg gtagttgaac atctacttca ctataggggc cagaggcgct ggctgtcacg
     3901 caaaattaca ccagtaattc cgcagccagc cgtgcaatca atacgttaga gagcaagaca
     3961 ggaacatcga gctgtttttg cagtaaatca cgatgacgtt ggtgaaatcc taaacaatcc
     4021 agcatgatga catcagcccc ttttgccagt aattctttcc cagcataaat gattttttgt
     4081 tctgaatcat gaatggggtt acccaatgaa aataccggcg atttctgcaa aatttgccat
     4141 ttttgcgcct gaacgggcag catctcctca accggaacga taacccccac ctgatgatct
     4201 tcaacaatag aggaaaccag tggaggcaat attcgcgacg gctcaagaaa gatcgtatta
     4261 cgcgcagtca tactactaat gtttgctgta ctcattaata aaatgacgtc ataaccctga
     4321 ttatcgagca cttcaaccac accttgcaga tctcgctcca ctttacgacg cgaaacatgg
     4381 gccagctggt tgtcatttaa taatgtgaga atggtgtctt cgcctgcttc tggcgcgtac
     4441 tccgccatca cttcttcacg acttaacttc cccagcaggc tatgatggga aatattatct
     4501 tcgtcaatgt attccgtcag gagcggcaaa acctcctgca taggtacaat gccgatggtc
     4561 aggatcgcca acgacgcact cattgttcat ccaccttact tttctttcgt cgttaattac
     4621 cgggcaagtg tgaagcacca ctgctgacac ttacttgtgc tacaaatgac aaaaagcgta
     4681 gcagcagacg tgcacggcat aatgtaaaga ttccaaatga ttccagtaat ggaatgttat
     4741 tgtttaatat tctaattatt agaaaaacat gaattatgaa aaatgtgacg cagatccctt
     4801 gtagtggcac tcagaatccc ttccgagcag tctgatgcgg tcactctttg tcagggtcct
     4861 catagcgttt tttcgcatcc atcgcttcct gagccgtcgg atgttcactg atcaacgagt
     4921 ctggtttagg atgatcggct ctgagttggt accaggttac cgtctgtcct ggctttcctt
     4981 tttcaatcgt cactatatat gcttcacgag gataaggcgg tttcgttggc atcgttcttc
     5041 cttatttcac gggatgaacg ttaagtatag gcgctctaaa atcaacaatt gatcgtctgt
     5101 gccagggcgc tacgaatttc agaaatcacc tggctgggtt cgtttgttgc gtcgatgata
     5161 atatgcgcaa cttcgcgata tagcgcatcg cgttcttcca gcacttcctg aacttcttcg
     5221 ctcagcggtt ttcccgttaa ggttggccgt aaatcttctt ccggtgcagc ttgcagtcgg
     5281 ttaaccagga ctgatactgg cgcacacaaa taaaccacga tcccgttatt ttgcatgaag
     5341 tgacgattaa attccgtcag aataatgccg ccgcctgtag cgataacggt ggatggcgca
     5401 gttaccgctt ccagcgccgc cgtttctctg gcgcgaaatc ccgcccactc ttccctttcg
     5461 acgatctccg cgaccgtcat attgagctgt gattgcaacc actgatcggt atcgacaaac
     5521 cgacggttaa gcgaatcggc aagggccatt ccgaccgttg ttttaccaca gccccgaggc
     5581 ccgatcagaa aaagaggttg tgtcatcgtg ggttttcccc aataggtcgc aatgcggcga
     5641 aagccggtgt catgagaata gcgatcatac catcaaacta gtacaatttc gattgtaaag
     5701 aaaaaattcc acttaaagtg aaaatctcaa tacatccctt actataccaa taaatattca
     5761 agaatgaagt gtaaataata aattacattt agccacgact acgttgcact tccagccacc
     5821 acttctccag ctccgcggca aaggcctggc ggtcacgttg tgaaaggcta tctggtccgc
     5881 cggtctggat cccactggca cgtaaggtat ccataaaatc gcgcatcgtc aggcgctcac
     5941 gaatggtcgc tggcgtgtaa cgttcgccgc gcggattaag cgccgctgcg cctttctcga
     6001 tggcttcagc agccaaaggt atatctgcgg tgatcaccaa atcgcccgct tcacactgcc
     6061 ggacaatttc gttatcggca acgtcgaaac ctgccgcgac gcgcagcgta cgaataaatc
     6121 gcgatggcgg cacgcgtaaa ctctggtttg ctaccagtac cagcggcatc tgcatacgtt
     6181 ccgccgcgcg atacaaaatc tctttaatta cattgggaca cgcgtcggca tccacccaaa
     6241 ttgtcataaa gtcatccttt gttgggtaat cctctattgt gtcgcgcttt tgccttccgg
     6301 catagttctg tttatgcttc tgccagcgat tatcaaaaca atgaatttca cggcaggagt
     6361 gaggcaatgg aaaagaaaat cggttttatt ggctgcggca atatgggaaa agccattctc
     6421 ggcggtctga ttgccagcgg tcaggtgctt ccagggcaaa tctgggtata caccccctcc
     6481 ccggataaag tcgccgccct gcatgaccag ttcggcatca acgccgcaga atcggcgcaa
     6541 gaagtggcgc aaatcgccga catcattttt gctgccgtta aacctggcat catgattaaa
     6601 gtgcttagcg aaatcacctc cagcctgaat aaagactctc tggtcgtttc tattgctgca
     6661 ggtgtcacgc tcgaccagct tgcccgcgcg ctgggccatg accggaaaat tatccgcgcc
     6721 atgccgaaca ctcccgcgct ggttaatgcc gggatgacct ccgtaacgcc aaacgcgctg
     6781 gtaaccccag aagataccgc tgatgtgctg aatattttcc gctgctttgg cgaagcggaa
     6841 gtaattgctg agccgatgat ccacccggtg gtcggtgtga gcggttcttc gccagcctac
     6901 gtatttatgt ttatcgaagc gatggccgac gccgccgtgc tgggcgggat gccacgcgcc
     6961 caggcgtata aatttgccgc tcaggcggta atgggttccg caaaaatggt gctggaaacg
     7021 ggagaacatc cgggggcact gaaagatatg gtctgctcac cgggaggcac caccattgaa
     7081 gcggtacgcg tactggaaga gaaaggcttc cgtgccgcag tgatcgaagc gatgacgaag
     7141 tgtatggaaa aatcagaaaa actcagcaaa tcctgatgac tttcgccgga cgtcaggccg
     7201 ccacttcggt gcggttacgt ccggctttct ttgctttgta aagcgccaaa tctgccgatt
     7261 tcaaccactc acgatagtga ctcatttgtg ggttcagcgg cgcaaccccc acactaatcc
     7321 gtaaagttac ctgtggcgta ttcggcaagc gtaacgtgtt tagcccttca cgcacccgta
     7381 acatggcggt aatggcgctc tcagctggcg taccggacat gattactgca aactcatcgc
     7441 cgccaaaccg accaatcaca tcgctaccgc gcagggtaat ttgtaactgt cgggtaagcg
     7501 ccacaatcgc ttcatcgccc acatcatgac cccaggtatc gttgatgctc ttgaaatggt
     7561 cgatatcgat aatcagtaac gttgcatcgc gattatgccg ccgacagtta tcaaattcat
     7621 tgcgtaacat agtttcccaa tgacgtcggt tatacacgcc cgtcatgccg tcgcgagtac
     7681 tcatgacctg caacctgcgt ttatgttccg ccagtttggt tgccgtctgg tagctgaccc
     7741 agccaaacag cagaggataa acgacaataa tgggaaggga gagccaccat tccagcggcg
     7801 cactattgaa cgacacggta atgcccgtaa gctcgagggt gacaaggcag gaaaccacca
     7861 tcaacaccag acccgcgaca aacagacggg ggccgcctgc ccccatcaaa ttcagacaca
     7921 taatcatcaa catagcggtg gaaggcagca cgtttacgcc cattacgcct acccacattc
     7981 ccgctaatac tgcatcggtt tttaagttgt aaatttcccg gctaagcgga tcgacggccc
     8041 tgctcgctat ctgccaagct aaatgcggcc agacgaacgc ccagccgacc aacaccagcc
     8101 accaccagcc cggcggcggg tgcgaaacca gcgttgaagc aatcggtaag aacatgccag
     8161 ccaggccaac cgcacggggt agtctgacgc gacgggcgaa gcgcagcccg gaccgctgat
     8221 gttcgttttg aggagttaaa ggaggttcct ccccatgcgc cgccgctttt ttgaaaaagt
     8281 tttcatcatt cattattttt gggaacattc agaaacaatt ttcccaaatt atagagacgg
     8341 ggctaaaggc agaagtatga aatttcgggt gagccacttt cgtagctcac ccgttagcac
     8401 tatcaggtat caggcggctt tcttgaggca ggcactcata aacttattac gatcatcacc
     8461 tttcagagat tgttgcgttg cttgattatt acattcgcgc atcttttgct gctgtggcgt
     8521 caaacttttt tcaccaggcg cagacttgct gttcttcagg caatcactca tgtaggtctt
     8581 acgagcatcc cctttcaacg cctgcgccgt cgcctgctga ttacaggagg tcatacgctg
     8641 ttgttgtggg gttaaggttc tctcggcagc gccgacggtg gttaaaaaaa ccagaccaaa
     8701 aagcaaggta accagtaatg ttattttcat agcaccatcc ctcttcatgt tttaaccatg
     8761 agcgtttgcg cccgtgatct gccattaagt ctggttgcta acagcaaaaa aaccacccgg
     8821 cagcgaaaat tcactgccgg gcgcggtttt atttcagccc cagagcggct ttcatggtgt
     8881 agaagagatc ggtctggtcg gtcagtccaa caacattggc ggcatgcggg ccatacgccg
     8941 caatacgcaa ctgactgccg gtatgttctt gtgaatcctc ttcggaattc ccgtaactca
     9001 tcaccatcac tgcgccatct ttagtattta gcgcctgagt gaggcccggc gctttggtat
     9061 ccggcgcaac aacctggctg gcgtgggcgt gatcagcggt gactatgacc agcgtgttac
     9121 cctccttttt agcgaattcc agcgcccgtt gtacggcttc atcgagatcg accgtctcgc
     9181 caatttgccc acaaggattc gcagcgtgat cctgtttatc gattgacgca ccttcaactt
     9241 gcaggaaaaa tcctttctca tttttactca acaattcaat ggctttgtcg gtcatctgtg
     9301 ccagggttgg tacactgtca ttacgttgcg gattaggcgt ataggtgact gcgggcttat
     9361 cgatattgcc gtggtacgtt gctttcggcc ctagccagcg cactggcata ttgccgtcag
     9421 caaacaggcc aagcaggggt ttttgctgat tcgcttccgt caccgaattc agtgaggcag
     9481 catcgttcac caactgataa ccacgcgcct gtgcctattc acgcagcgtt tttccctgcc
     9541 attcaccagc ggttgccgtt tcagcaaagg tttttgcgcc gccgccaagc gtaacgtcgg
     9601 cacgagcgtt aagcagctgt tcggtaatcg atccttttcc gcctttttcc agagcgttac
     9661 ccggacattt ttcactggtc gcgctcggac cgtagcattt gcgcgaagtc acatgtgcca
     9721 ccagcgcagc gggcgtggca tcctgcaact ctgcggtaga aacgttaccg gtcgccagac
     9781 ctgcggcttt tgccatttcc agaatcgttg ggtgatcttt ttcgtgaata tcgacgccca
     9841 gcgcgccgtt ataggttttg acaccggttg accaggcggt tgctgatgca gccgagtcgg
     9901 tgacgtagtc cggtttgcca gtttttttat tcagcg
//
LOCUS       DBKEKI010000145         9919 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.146, whole genome shotgun sequence.
ACCESSION   DBKEKI010000145 DBKEKI010000000
VERSION     DBKEKI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.146"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            560..2128
                     /gene="cydA"
                     /locus_tag="ACUQPX_003515"
     CDS             560..2128
                     /gene="cydA"
                     /locus_tag="ACUQPX_003515"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HHV9058089.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            2144..3283
                     /gene="cydB"
                     /locus_tag="ACUQPX_003516"
     CDS             2144..3283
                     /gene="cydB"
                     /locus_tag="ACUQPX_003516"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HHV9058090.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3298..3411
                     /gene="cydX"
                     /locus_tag="ACUQPX_003517"
     CDS             3298..3411
                     /gene="cydX"
                     /locus_tag="ACUQPX_003517"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HHV9058091.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3411..3704
                     /gene="ybgE"
                     /locus_tag="ACUQPX_003518"
     CDS             3411..3704
                     /gene="ybgE"
                     /locus_tag="ACUQPX_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HHV9058092.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3854..4258
                     /gene="ybgC"
                     /locus_tag="ACUQPX_003519"
     CDS             3854..4258
                     /gene="ybgC"
                     /locus_tag="ACUQPX_003519"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HHV9058093.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4255..4947
                     /gene="tolQ"
                     /locus_tag="ACUQPX_003520"
     CDS             4255..4947
                     /gene="tolQ"
                     /locus_tag="ACUQPX_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HHV9058094.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4951..5379
                     /gene="tolR"
                     /locus_tag="ACUQPX_003521"
     CDS             4951..5379
                     /gene="tolR"
                     /locus_tag="ACUQPX_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HHV9058095.1"
                     /translation="MARARGRGCRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5444..6685
                     /gene="tolA"
                     /locus_tag="ACUQPX_003522"
     CDS             5444..6685
                     /gene="tolA"
                     /locus_tag="ACUQPX_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HHV9058096.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKVAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            6818..8110
                     /gene="tolB"
                     /locus_tag="ACUQPX_003523"
     CDS             6818..8110
                     /gene="tolB"
                     /locus_tag="ACUQPX_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HHV9058097.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGLGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            8145..8666
                     /gene="pal"
                     /locus_tag="ACUQPX_003524"
     CDS             8145..8666
                     /gene="pal"
                     /locus_tag="ACUQPX_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HHV9058098.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8676..9467
                     /gene="cpoB"
                     /locus_tag="ACUQPX_003525"
     CDS             8676..9467
                     /gene="cpoB"
                     /locus_tag="ACUQPX_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HHV9058099.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNATIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9632..9707
                     /locus_tag="ACUQPX_003526"
     tRNA            9632..9707
                     /locus_tag="ACUQPX_003526"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9665..9667,aa:Lys,seq:ttt)
     gene            9743..9818
                     /locus_tag="ACUQPX_003527"
     tRNA            9743..9818
                     /locus_tag="ACUQPX_003527"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9776..9778,aa:Val,seq:tac)
     gene            9821..9896
                     /locus_tag="ACUQPX_003528"
     tRNA            9821..9896
                     /locus_tag="ACUQPX_003528"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9854..9856,aa:Lys,seq:ttt)
BASE COUNT         2432 a         2347 c         2661 g         2479 t
ORIGIN      
        1 gatttatgcc tttattaata atcctgaaac tctgcgtcgt attagccagt gaccaaaaaa
       61 agaattaagg tcaaccgtgc tgtttttgct tcgtctcttt ttatctttaa ttgccaaccg
      121 aaactaattt cagccttata attcacacat tttaaacata aatgtcacta aagttacctt
      181 attgaaacat gattaacata atttgtagaa attgatattt atcaatgtat aagtcttgga
      241 aatgggcatc aaaaagagat aaattgttct cgatcaaatt ggctgaaagg cggcaattta
      301 gctataaatt gatcaccgtc gaaaaatgca aatttgcttc aacaaaaacc tgtttattgt
      361 aaggattttg cggcgtaata tatacgtggg atcaatttga gtttttatta acatgtttgc
      421 aacctttctt tacgccgttt ttgtgtgcat tcacatggta tgatgaaagt gttcaaacaa
      481 atttctattg gggcatgcgt gtgacccttt ctaacggggt tcactctcgg agtcttcatg
      541 cgatgagcaa ggagtcatga tgttagatat agtcgaactg tcgcgcttac agtttgcctt
      601 gaccgcgatg taccacttcc tttttgtgcc actgacgctc ggtatggcgt tcctcctggc
      661 cattatggaa acggtctacg tcctctccgg caaacagatt tataaagata tgaccaagtt
      721 ctggggcaag ttgtttggta tcaacttcgc tctgggtgtg gctaccggtc tgaccatgga
      781 gttccagttc gggactaact ggtcttacta ttcccactat gtaggggata tcttcggtgc
      841 gccgctggca atcgaaggtc tgatggcctt cttcctcgaa tccacctttg taggtctgtt
      901 cttcttcggt tgggatcgtc tgggtaaagt tcagcatatg tgtgtcacct ggctggtggc
      961 gctcggttca aacctgtccg cactgtggat tctggttgcg aacggctgga tgcaaaaccc
     1021 aatcgcgtcc gatttcaact ttgaaactat gcgtatggag atggtgagct tctccgagct
     1081 ggtgcttaac ccggttgctc aggtgaaatt cgttcacact gtagcgtctg gttatgtgac
     1141 tggcgcgatg ttcatcctcg gtatcagcgc atggtatatg ctgaaaggtc gtgacttcgc
     1201 cttcgctaaa cgctcctttg ctatcgctgc cagcttcggt atggctgctg ttctgtctgt
     1261 tattgttctg ggtgatgaat ccggctacga aatgggcgac gtgcagaaaa ccaaactggc
     1321 tgctattgaa gccgagtggg aaacgcaacc tgcgcctgct gcctttactc tgttcggcat
     1381 tcctgatcag gaagaggaga cgaacaaatt tgcgattcag atcccttacg cactgggcat
     1441 cattgcaacg cgttccgtgg ataccccggt tatcggcctg aaagagctga tggtgcagca
     1501 tgaagaacgc attcgtaacg ggatgaaggc gtactctctg ctcgaacaac tgcgttctgg
     1561 ttctaccgac caggcggttc gtgaccagtt caatagcatg aagaaagacc tcggttacgg
     1621 tctgctgctg aaacgctata cgccaaacgt ggctgatgcg actgaagcgc agattcaaca
     1681 ggcaaccaaa gactccatcc cgcgtgtagc gccgctgtac tttgcgttcc gtatcatggt
     1741 ggcgtgtggc ttcctgctgc tggcaatcat cgcgctctct ttctggagtg tcatccgcaa
     1801 ccgcattggc gagaaaaaat ggcttctgcg cgccgcgctg tacggtattc cgctgccgtg
     1861 gattgctgta gaagcgggct ggttcgtggc tgaatatggc cgccaaccgt gggctatcgg
     1921 tgaagtgctg ccgacagctg tggcgaattc gtcactgacc gcaggcgatc tcatcttctc
     1981 aatggtgctg atttgcggcc tgtataccct gttcctggtg gcagaattgt tcttaatgtt
     2041 caagtttgca cgcctcggcc caagcagcct gaaaaccggt cgctatcact ttgagcagtc
     2101 ttccacgact actcagccgg cacgctaaga caggagtcgt caaatgatcg attatgaagt
     2161 attgcgtttt atctggtggc tgctggttgg cgttctgctg attggttttg cagtcactga
     2221 cggtttcgac atgggggtgg gcatgctcac ccgtttcctc ggtcgtaacg acaccgagcg
     2281 tcgaattatg attaactcca ttgcaccaca ctgggacggt aaccaggttt ggctgatcac
     2341 cgcgggcggc gcactctttg ctgcctggcc gatggtctat gccgctgcgt tctccggctt
     2401 ctatgtggcg atgatcctcg tgctggcgtc tttgttcttc cgtccggtcg gttttgacta
     2461 ccgctccaag attgaagaaa cccgctggcg taacatgtgg gactggggca tcttcattgg
     2521 tagcttcgtt ccgccgctgg taattggtgt agcgttcggt aacctgttgc agggcgtacc
     2581 gttcaacgtt gatgaatatc tgcgtctgta ctacaccggt aacttcttcc agttgcttaa
     2641 cccgttcggc ctgctggcag gcgtggtgag cgtagggatg atcattactc agggcgcaac
     2701 ctatctgcaa atgcgtaccg tgggcgaact gcacctgcgt acccgtgcaa cggctcaggt
     2761 ggctgcgctg gtgacactgg tctgtttcgc actggctggc gtatgggtga tgtacggtat
     2821 cgatggttat gtcgtgaaat cgacaatgga ccattacgca gcctctaacc cactgaataa
     2881 agaagtggtt cgtgaagctg gcgcatggct ggttaacttc aacaacacgc caattctgtg
     2941 ggctattccg gcactgggtg tggttctgcc gctgctgacc atcctgactg cacgtatgga
     3001 taaagccgcg tgggcgtttg tgttctcctc cctgacgctg gcctgcatca tcctgacagc
     3061 cggtatcgca atgttcccgt ttgtgatgcc gtccagcacc atgatgaacg caagtctgac
     3121 aatgtgggat gcaacttcca gccagctgac gcttaacgtc atgacctggg ttgcggtggt
     3181 tctggtaccg atcattctgc tctacaccgc ctggtgttac tggaaaatgt tcggtcgtat
     3241 caccaaagaa gatattgaac gtaacaccca ctctctgtac taagtaagga gctaaaaatg
     3301 tggtatttcg catggattct gggaacgctt cttgcctgtt cgtttggggt aatcaccgcg
     3361 ctggcgcttg aacacgtcga atcaggcaaa gccggtcaag aagacatctg atgagtaaga
     3421 ttatcgcgac cttgtatgcg gtaatggaca agcgccccct gcgggcgctt tccttcgtga
     3481 tggcgcttct gttagcagga tgtatgtttt gggacccatc tcgttttgcc gcgaagacca
     3541 gtgaactgga aatctggcat ggtttattgc tgatgtgggc cgtctgtgct ggtgtgattc
     3601 acggcgtggg ctttcgtccg cagaaggttc tttggcaagg gattttttgc ccattgcttg
     3661 ccgatattgt tctcattgtc gggctgattt tcttcttctt ttaaatcaga attctcttta
     3721 aaaaattatg ggccgctcca ggcccataaa tttttacgct cccttaactt gccctcattc
     3781 ccaaacctca atcgcgcgcg tatagtagca gcgtttaaaa gctctaactt ttgttgcatt
     3841 accgggatgt aaagtgaata caacgctgtt tcgatggccg gttcgcgtct actatgaaga
     3901 taccgatgcc ggtggtgtgg tgtaccacgc cagttacgtc gctttttatg aaagagcacg
     3961 cacagagatg ctgcgtcatc atcacttcag tcagcaggcg ctgatggctg aacgcgttgc
     4021 ctttgtggta cgtaaaatga cggtggaata ttacgcacct gcgcggctcg acgatatgct
     4081 cgaaatacag actgaaataa catcaatgcg tggcacctct ttggttttca cgcaacgtat
     4141 tgtcaacgcc gagaatactt tgctgaatga agcagaggtt ctggttgttt gcgttgaccc
     4201 actcaaaatg aagcctcgtg cgcttcccaa gtctattgtc gcggagttta agcagtgact
     4261 gacatgaata tccttgattt gttcctgaag gctagccttc tggttaaact tatcatgttg
     4321 attttgattg gtttttcaat cgcatcctgg gccattatta tccagcgaac ccgtattctt
     4381 aacgcagcgg cgcgtgaagc cgaagcgttt gaagataaat tctggtctgg aatcgaactc
     4441 tctcgcctct atcaagagag tcaggggaaa cgggataatc tgactggttc ggaacaaatc
     4501 ttttacagcg ggttcaaaga gtttgtgcgc ctgcatcgtg ccaatagcca tgcgccggaa
     4561 gccgtagtgg aaggggcgtc gcgtgctatg cgtatctcca tgaaccgtga acttgaaaat
     4621 ctggaaacgc acattccgtt cctcggtacg gttggctcca tcagcccgta tattggtctg
     4681 tttggtacgg tctgggggat catgcacgcc tttatcgccc tcggggcggt aaaacaagca
     4741 acactgcaaa tggttgcgcc cggtatcgca gaagcgttga ttgcgacggc aattggtctg
     4801 ttcgccgcaa ttccggcggt aatggcttat aaccgtctca accagcgcgt aaacaaactg
     4861 gaactgaatt acgacaactt tatggaagag tttaccgcga ttctgcaccg ccaggcgttt
     4921 accgttagcg agagcaacaa ggggtaagcc atggccagag cgcgtggacg aggttgtcgc
     4981 gatctcaagt ccgaaatcaa cattgtaccg ttgctggacg tactgctggt gctgttgctg
     5041 atctttatgg cgacagcgcc catcatcacc cagagcgtgg aggtcgatct gccagacgct
     5101 actgaatcac aggcggtgag cagtaacgat aatccgccag tgattgttga agtgtctggt
     5161 attggtcagt acaccgtggt ggttgagaaa gatcgcctag agcgtttacc accagagcag
     5221 gtggtggcgg aagtgtccag ccgtttcaag gccaacccga aaacggtctt tctgatcggt
     5281 ggcgcaaaag atgtgcctta cgatgaaata attaaagcac tgaacttgtt acatagtgcg
     5341 ggtgtgaaat cggttggttt aatgacgcag cctatctaaa catctgcgtt tcccttgctt
     5401 gaaagagagc gggtaacagg cgaacagttt ttggaaaccg agagtgtcaa aggcaaccga
     5461 acaaaacgac aagctcaagc gggcgataat tatttcagca gtgctgcatg tcatcttatt
     5521 tgcggcgctg atctggagtt cgttcgatga gaatatagaa gcttcagccg gaggcggcgg
     5581 tggttcgtcc atcgacgctg tcatggttga ttcaggtgcg gtagttgagc agtacaaacg
     5641 catgcaaagc caggaatcaa gcgcgaagcg ttctgatgaa cagcgcaaga tgaaggaaca
     5701 gcaggctgct gaagaactgc gtgagaaaca agcggctgaa caggaacgcc tgaagcaact
     5761 tgagaaagag cggttagcgg ctcaggagca gaaaaagcag gctgaagaag ccgcaaaaca
     5821 ggccgagtta aagcagaagc aagctgaagt ggcggcagcg aaagcggcgg cagatgctaa
     5881 agctgcggaa gaagcagcga agaaagcggc tgcagacgcg aagaaaaaag cagaagcaga
     5941 agccgccaaa gccgcagccg aagcgcagaa aaaagccgag gtagccgctg cggcactgaa
     6001 gaagaaagcg gaagcggcag aagcagctgc agctgaagca agaaagaaag cggcaactga
     6061 agctgctgaa aaagccaagg cagaagctga gaagaaagcg gctgctgaaa aggctgcagc
     6121 tgataagaaa gcggcagcag agaaagctgc agccgacaaa aaagtagcag aaaaagcggc
     6181 tgctgataag gcagcagctg ataagaaagc agcggcagaa aaagccgccg cagacaaaaa
     6241 agcggcagcg gcaaaagcag cagctgaaaa agccgctgca gcaaaagctg ccgcagaggc
     6301 agatgatatt ttcggtgagc taagctctgg taagaatgca ccgaaaacgg ggggaggggc
     6361 gaaagggaac aatgcttcgc ctgccgggag tggtaatact aaaaacaatg gcgcatcagg
     6421 ggccgatatc aataactatg ccgggcagat taaatctgct atcgaaagta agttctatga
     6481 cgcatcgtcc tatgcaggca aaacctgtac gctgcgcata aaactggcac ccgatggtat
     6541 gttactggat atcaaacctg aaggtggcga tcccgcactt tgtcaggctg cgttggcagc
     6601 agctaaactt gctaagatcc cgaaaccacc aagccaggca gtatatgaag tgttcaaaaa
     6661 cgcgccattg gacttcaaac cgtaatcgcg atgttgactg ttcggacggt caacatcagg
     6721 caccggttgc cacggggttc tggtagtttt gtgtatttta gtttgttaac attctgctaa
     6781 attatcgtgg gccatcggtc cagataaggg agatatgatg aagcaggcat tacgagtagc
     6841 atttggtttt ctcatactgt gggcatcagt tctgcatgct gaagtccgca ttgtgatcga
     6901 cagcggtgta gattccggtc gtcctattgg tgttgttcct ttccagtggg cggggcttgg
     6961 tgcggcacct gaagatattg gcggcatcgt tgctgctgac ttgcgtaaca gcggtaaatt
     7021 taatccgtta gatcgcgctc gtctgccaca gcagccgggt agtgcgcagg aagtacaacc
     7081 agctgcatgg tccgcactgg gcattgacgc tgtagttgtc ggtcaggtca ctccgaatcc
     7141 ggatggttct tacaatgttg cttatcaact tgttgacact ggcggcgcac cgggtactgt
     7201 acttgctcag aactcgtaca aagtgaacaa gcagtggctg cgttatgctg gtcataccgc
     7261 cagtgatgaa gtgtttgaaa aactgaccgg cattaaaggt gcgttccgta cccgtattgc
     7321 ctacgttgtt cagaccaacg gcggtcagtt cccgtatgaa ctgcgcgtat ctgactatga
     7381 cggttacaac cagtttgtcg ttcaccgttc accgcagccg ctgatgtctc cggcgtggtc
     7441 accagacggt tctaaactgg cttatgtgac cttcgaaagc ggtcgttccg cgctggttat
     7501 tcagacgctg gcaaatggcg ctgtacgtca ggtggcttca ttcccgcgtc acaacggtgc
     7561 acctgcattc tcgccagacg gcagcaaact ggcattcgcc ttgtcgaaaa ccggtagtct
     7621 gaacctgtac gtaatggatt tggcttctgg tcagatccgc caggtgactg atggtcgcag
     7681 taacaatacc gaaccgacct ggttcccgga tagccagaac ctggcattta cttctgacca
     7741 ggccggtcgt ccgcaggttt ataaagtgaa tatcaacggc ggtgcgccac aacgtattac
     7801 ctgggaaggt tcgcagaacc aggatgcgga tgtcagcagc gacggtaaat ttatggtaat
     7861 ggtcagctcc aatggtgggc agcagcacat tgccaaacaa gatctggcaa cgggaggcgt
     7921 acaagttctg tcgtccacgt tcctagatga aacgccaagt ctggcaccta acggcactat
     7981 ggtaatctac agctcttctc aggggatggg atccgtgctg aatttggttt ctacagatgg
     8041 gcgtttcaaa gcgcgtcttc cggcaactga tggacaggtc aaattccctg cctggtcgcc
     8101 gtatctgtga taataattaa ttgaatagta aaggaatcat tgaaatgcaa ctgaacaaag
     8161 tgctgaaagg gctgatgatt gctctgcctg ttatggcaat tgcggcatgt tcttccaaca
     8221 agaacgccag caatgacggc agcgaaggca tgctgggtgc cggcactggt atggatgcga
     8281 acggcggcaa cggcaacatg tcttccgaag agcaggctcg tctgcaaatg caacagctgc
     8341 agcagaacaa catcgtttac ttcgatctgg acaagtacga tatccgttct gacttcgctc
     8401 aaatgctgga tgcacatgca aacttcctgc gtagcaaccc gtcttacaaa gtcaccgtag
     8461 aaggtcacgc ggacgaacgt ggtactccgg aatacaacat ctccctgggt gaacgtcgtg
     8521 cgaacgccgt taagatgtac ctgcagggta aaggcgtttc tgcagaccag atctccatcg
     8581 tttcttacgg taaagaaaaa cctgcagtac tgggtcatga cgaagcggca tactccaaaa
     8641 accgtcgtgc ggtactggtt tactaagaga attgcatgag cagtaacttc agacatcaac
     8701 tattgagtct gtcgttactg gttggtatag cggccccctg ggccgctttt gctcaggcac
     8761 caatcagtag tgtcggctca ggctcggtcg aagaccgcgt cactcaactt gagcgtattt
     8821 ctaatgctca cagccagctt ttaacccaac tccagcaaca actttctgat aatcaatccg
     8881 atattgattc cctgcgtggt cagattcagg aaaatcagta tcaactgaat caggtcgtgg
     8941 agcggcaaaa gcagatcctg ttgcagatcg acagcctcag cagcggtggt gcagcggcgc
     9001 aatcaaccag cagcgatcaa agcggtgcgg cggcatcaac gacgccgaca gctgatgctg
     9061 gtactgcgaa tgctggcgcg ccggtgaaaa gcggcgatgc aaacacggat tacaatgcaa
     9121 ctattgcgct ggtgcaggat aaatcccgcc aggatgacgc aatggtggca tttcagaatt
     9181 tcatcaaaaa ttaccctgat tcaacttacc tgccaaacgc caattattgg ctgggtcagt
     9241 taaactacaa caagggtaaa aaagatgatg cggcgtacta ttttgcttcg gtagtgaaaa
     9301 actatccgaa gtcaccaaag gctgctgatg cgatgtttaa agtcggcgtc atcatgcagg
     9361 acaaaggtga caccgcaaaa gcgaaagccg tataccagca ggttatcagt aaataccctg
     9421 gtactgatgg cgctaaacag gcacaaaaac gtctgaacgc gatgtaatgc ataacacacg
     9481 accagaagtc gcattatttc tggtcgtgtc gtgcgaatca taagcagttg agtgatctgc
     9541 atcgaaattt ttgttgcgct caagtctgaa atcagtaata tatgccgccg ttgccacggg
     9601 atatcaaaca aaccgaaagc aacgaaaaag tgggtcgtta gctcagttgg tagagcagtt
     9661 gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccacta acatagttag
     9721 ttgtagtatc cagcgtagtg tcgggtgatt agctcagctg ggagagcacc tcccttacaa
     9781 ggagggggtc ggcggttcga tcccgtcatc acccaccacc gggtcgttag ctcagttggt
     9841 agagcagttg acttttaatc aattggtcgc aggttcgaat cctgcacgac ccaccactaa
     9901 ttacggtggg ttcggtgga
//
LOCUS       DBKEKI010000146         9872 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.147, whole genome shotgun sequence.
ACCESSION   DBKEKI010000146 DBKEKI010000000
VERSION     DBKEKI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.147"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..517
                     /locus_tag="ACUQPX_003529"
     CDS             <1..517
                     /locus_tag="ACUQPX_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HHV9058100.1"
                     /translation="SILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVKAT
                     GSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSWLVQPVRSEET
                     LHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYKSA
                     ESVVQCLGRPD"
     gene            603..1193
                     /gene="prfH"
                     /locus_tag="ACUQPX_003530"
                     /pseudo
     CDS             603..1193
                     /gene="prfH"
                     /locus_tag="ACUQPX_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1250..2707)
                     /gene="pepD"
                     /locus_tag="ACUQPX_003531"
     CDS             complement(1250..2707)
                     /gene="pepD"
                     /locus_tag="ACUQPX_003531"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HHV9058101.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2969..3427
                     /gene="gpt"
                     /locus_tag="ACUQPX_003532"
     CDS             2969..3427
                     /gene="gpt"
                     /locus_tag="ACUQPX_003532"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HHV9058102.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3519..4763
                     /gene="frsA"
                     /locus_tag="ACUQPX_003533"
     CDS             3519..4763
                     /gene="frsA"
                     /locus_tag="ACUQPX_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HHV9058103.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4821..5222
                     /gene="crl"
                     /locus_tag="ACUQPX_003534"
     CDS             4821..5222
                     /gene="crl"
                     /locus_tag="ACUQPX_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HHV9058104.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5261..6316)
                     /gene="phoE"
                     /locus_tag="ACUQPX_003535"
     CDS             complement(5261..6316)
                     /gene="phoE"
                     /locus_tag="ACUQPX_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HHV9058105.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            6604..7707
                     /gene="proB"
                     /locus_tag="ACUQPX_003536"
     CDS             6604..7707
                     /gene="proB"
                     /locus_tag="ACUQPX_003536"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HHV9058106.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7719..8972
                     /gene="proA"
                     /locus_tag="ACUQPX_003537"
     CDS             7719..8972
                     /gene="proA"
                     /locus_tag="ACUQPX_003537"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HHV9058107.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9087..9162
                     /locus_tag="ACUQPX_003538"
     tRNA            9087..9162
                     /locus_tag="ACUQPX_003538"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9120..9122,aa:Thr,seq:cgt)
     gene            complement(9177..>9872)
                     /locus_tag="ACUQPX_003539"
     CDS             complement(9177..>9872)
                     /locus_tag="ACUQPX_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HHV9058108.1"
                     /translation="LRAAEREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPE
                     PLKSVVEFALATGLRRSNIINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRV
                     LKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDL
                     RHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPN
                     LSQSKNKEGTNDV"
BASE COUNT         2362 a         2457 c         2589 g         2464 t
ORIGIN      
        1 gtctatttta cagcggcata ttgcctcgtt ttcgcatcat ggtttgcctg aaggcagtga
       61 cgacgcgctg gcgtttgcgc gttttaatcg ccacctgatc gctttgcgca taatgcaaca
      121 ggttaaggcc accggaagtc cggttctgga tgtggcgcat aactttgtta gcgcgtgtcg
      181 aatcggtgat caacaagggg tcttacatcg taaaggtgcc acgcctgatg actgtggtct
      241 ggtggttatt cccggttcac gcggtgatta ctcctggctg gttcagcccg tcaggagtga
      301 ggaaacattg cattcgctgg cgcatggggc tgggcgtaaa tgggggcgca ccgagtgtaa
      361 agggcgtctg gcagcgaaat acacagcgac gcaactctcg cgtactgaac ttggcagtcg
      421 ggtaatttgt cgcgataaac aactcatctt cgaagaagcg ccacaagcct ataaatcggc
      481 tgaaagtgtg gtgcaatgtc tgggcaggcc ggattaatta ttcctgtcgc gcggctgcgt
      541 ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta taactctcct
      601 cagtgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggac aggctaatta
      661 aagaagctgc ccgacaagac gtcgcggtaa cggtgctgga aacagaaaca ggccgctact
      721 ctgacacgct gcgttcggcg ctgatttctc tggatggcga caacgcatgg gcgttaagcg
      781 aaagctggtg tggcactatt cagtggattt gcccgagccc gtatcgccca catcacgggc
      841 gtaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggag caatccgatg
      901 cgatccggta tgagacgctg cgttcgtcgg ggccgggcgg tcaacatgtc aataaaaccg
      961 actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt cagtcagagc
     1021 gcagccagca tgctaacaaa cggctggcgc gattgctgat tgcctggaag ctggatcagc
     1081 agcaatagga aaatagcgcg gtgctgaaat cacagcggcg aatgttccat caccagattg
     1141 aacgtggcaa cccgcgacga acgtttacag ggatggcttt tatcgaagga taatgaagga
     1201 tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat tacttcgccg
     1261 gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt tcgatgtgaa
     1321 cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc atgtccattt
     1381 ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc tggatgttcg
     1441 gcgtcttgtt gaacaggcgc tgataggttt cacgtaccag atgcatcacc ggagaattag
     1501 cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca gccagtttac
     1561 ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcagt gaacggatca
     1621 ggcagtgaat ttctacgtta ttgtcagtca tggtcaccac accgacgttc agggaggttt
     1681 cgaccacacc ttttgccacg tcggaattgc ggatcacacc gttcggggtg gcgttcagca
     1741 gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc gctacagagt
     1801 ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg atctcctgat
     1861 aggtattcac cagagatttc agtgcgtcga ctttatcagc tgcgacagca atggtcgcaa
     1921 aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata aggcgcaggt
     1981 ccagttcttc tgcatgaccc gccaggaagc gcaccagcag tttgttggca ttacccaggc
     2041 caacgtggat ttcaccgccg gagtgaccgc ctttcagacc ttttaaggtt aacttgaagg
     2101 tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg aagtcgatac
     2161 cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta atcagaatat
     2221 cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct tcttcggtca
     2281 tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga accgccagtg
     2341 cagaggccat accaatgccg ttatccgcac ccagcgtggt gccgcgcgct ttaacccatt
     2401 cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg tcgttatttt
     2461 tctgcggcac catatcgagg tgggcctgta agacgaccgg tttacgattt tccatacctg
     2521 cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca tggaaacctt
     2581 tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag gacgggtgag
     2641 gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat tgagacagtt
     2701 cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt cacagggtta
     2761 gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtattgagaa taccacttta
     2821 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
     2881 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
     2941 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
     3001 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
     3061 cattattgcc gtaagccgtg gcggtctggt accgggtgcg ttactggcgc gtgaactggg
     3121 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
     3181 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
     3241 ggataccggt ggtactgctg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
     3301 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
     3361 agatacctgg atcgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
     3421 tcgctaatct tttcaacgcc tggcattgcc gggcgttgtt ctttttaact tcaggcaggt
     3481 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
     3541 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     3601 atcacggcgc acaaccgcct gtgcagtcgg ctcttgatgg taaaaccatc cctcactggt
     3661 atcgcatgat taaccgtctg atgtggatct ggcgcggtat tgacccacgt gaaatcctcg
     3721 acgtccaggc acgtattgtg atgagcgatg ctgaacgtac cgacgatgat ttatacgata
     3781 ccgtgattgg ctaccgtggt ggcaactgga tttatgagtg ggccacccag gcgatggtgt
     3841 ggcaacaaaa agcctgtacg gaagaagatc cgcaactcag tggtcgacac tggctgcatg
     3901 cggctacgtt gtacaacatt gccgcctatc ctcatctgaa aggagatgac ctggccgagc
     3961 aagcgcaggc tttatcaaac cgcgcctatg aagaggccgc ccagcgtctg ccgggcacga
     4021 tgcggcagat ggagtttacc gtacccggcg gtgcgcccat caccggcttt ttgcatatgc
     4081 cgaaaggcga tggcccgttc ccgacagtat taatgtgtgg tggtctggat gcgatgcaga
     4141 cggactatta cagcctgtat gaacgttatt ttgcgccgcg cggcattgcg atgctgacta
     4201 ttgatatgcc gtcggtgggc ttttcttcaa aatggaagct cacccaggac tccagcctgt
     4261 tgcatcagca cgtcttaaag gcgctgccta acgtaccgtg ggtggatcac actcgcgtcg
     4321 cggcctttgg tttccgtttc ggcgctaacg ttgccgtgcg tctggcatac cttgaatcgc
     4381 cgcgtctgaa agcggttgcc tgtcttggtc cggtagttca taccctgttg agtgatttta
     4441 agtgccagca acaggtgccg gaaatgtatc ttgacgttct ggcgagtcgt ttggggatgc
     4501 atgatgcttc cgacgaagcg ttacgcgtgg agctgaatcg ctattcatta aaagtgcaag
     4561 gattgctggg gcgtcgctgc ccaacgccaa tgttatcagg ctactggaag aacgatccgt
     4621 tcagcccgga agaggactca cgcttaatca cctcatcatc tgctgacggt aaattattag
     4681 agatcccatt taacccggtg tatcggaatt ttgacaaagg tcttcaggaa atcaccggct
     4741 ggatcgaaaa acgcttgtgt taaaaatttg ctaaattttg ccaatttggt aaaacagttg
     4801 catcacaaca ggagatagca atgacgttac cgagtggaca cccgaagagc agattgatca
     4861 aaaaatttac cgcactaggc ccgtatattc gtgaaggtaa gtgcgaagat aatcgattct
     4921 ttttcgattg tctggctgta tgcgtcaacg tgaaaccggc accggaagtg cgtgaattct
     4981 ggggctggtg gatggagctg gaagcgcagg aatcccgttt tacctacagc taccagtttg
     5041 gtctgttcga taaagcaggc gactggaaga gtgttccggt aaaagatact gaagtggttg
     5101 aacgactgga gcacaccctg cgtgagtttc acgagaagct gcgtgaactg ctgacgacgc
     5161 tgaatctgaa gctggaaccg gcggatgatt ttcgtgatga accggtgaag ttaacggcgt
     5221 gagtgaaatg tgccggatgc atcacatccg gcaatattca ttaaaactga tacgtcatcc
     5281 caaccgcgac agtatcatca ttattaatat taagcttatt atcgctatcc agttggttga
     5341 ttttataatc aacaaacgct gacatatttt tgttgaaata atacgtagca ccgacgtcga
     5401 tataattgac cagatcttca tcaccgatgc cctcaatatc tttccctttc gataagacat
     5461 aacccagcga tggacgcaga ccaaagtcaa actggtattg agcgaccgct tcaaagttct
     5521 gtgtcttatt ggcaaagccg ccagttattg gcgtcatttt gcgtgtttca gaatagaaag
     5581 ttgccagata aatattattg gcatcgtatt tcagacctgt tgcccatgct tctgcacgct
     5641 tgcctgtgcc acggctttgc aggttctgct cgttggtgcg atctgagttg gtataggccc
     5701 cactaatggc gaaatcgctg ccgccaaagt catatgtcaa tgacgtgccg aagccatcgc
     5761 cgttttgctt tttaacgtcg cggttttcgt ttttcccttg atattgcagg gttaagttca
     5821 ggccatcgat aacgccgaag aagtcggtgt tccgatacgt cgccagaccg ctggcgcgtt
     5881 tggtcataaa gttgtcggtc tgcgccgagg aatcgccacc aaattccggg aacatatcgg
     5941 tccaggcttc cacgtcatac aacgcgccca ggttacgacc ataatcgaaa gaacccaaat
     6001 ctttatattt caacccggca aaagcgagac gcgttttttg ctgtgcagta tcactctccg
     6061 ctttatttcc ggcaaactcc gcttcccacc gaccataacc agtcagttga tcgttaattt
     6121 gagtttcacc tttaaaacca aaacggatat aactctggtc gccatcttta ctggcgttat
     6181 cactcatata atgcatggct ttaactttgc catagacatc cagtttatta ccgtctttat
     6241 tatatatttc tgcagcctgt acagatgcag atgccacaat gcccatcacc actaatgcca
     6301 gagtgctctt tttcattttc attcctgatt ttaattaacg cgcgaatatt cagcgggaga
     6361 gccccgttga aaacaggaaa gtttttaacc tgagattgtt aaagatatat tacagattaa
     6421 tgatattctg aaaatgtggt aatttattaa atctgtaata aaagcgtaaa caactgccgc
     6481 tacgcttgct gatcccgcgc aacaaaacgc catgctttgc tcgcagatgg ttggcaaccg
     6541 acgacagtcc tgctaaaacg ttcgtttgat atcatttttc ctaaaattga atggcagaga
     6601 atcatgagtg acagccagac gctggtggta aaactcggca ccagtgtgct aacaggcgga
     6661 tcgcgccgtc tgaaccgtgc ccatatcgtt gaacttgttc gccagtgcgc gcagttacat
     6721 gccgccgggc atcggattgt tattgtgacg tcgggcgcga tcgccgccgg acgtgagcac
     6781 ctgggttacc cggaactgcc agcgactatc gcctcgaaac aactgctggc ggcggtaggg
     6841 cagagtcgac tgattcaact gtgggaacag ctgttttcga tttatggcat tcacgtcggg
     6901 caaatgctgc tgacccgtgc tgatatggaa gaccgtgaac gcttcctgaa cgcccgcgac
     6961 accctgcgtg cgttgctcga taacaatatc gttccggtaa tcaatgagaa cgatgctgtc
     7021 gctacggcag agattaaggt cggcgataac gataaccttt ctgcgctggc ggcgattctg
     7081 gcgggtgccg ataaactgtt gctgctgacc gatcaaaaag gtttgtatac cgctgatccg
     7141 cgcagcaatc cgcaggcaga actgattaaa gatgtttacg gcattgatga cgcactgcgc
     7201 gcgattgccg gtgacagcgt ttcaggcctc ggaactggcg gcatgagtac caaattgcag
     7261 gccgctgacg tggcttgccg tgcgggtatc gacaccatta ttgccgcggg cagcaagccg
     7321 ggcgttattg gtgatgtgat ggaaggcatt tccgtcggta cgctgttcca tgcccaggcg
     7381 actccgcttg aaaaccgtaa acgctggatt ttcggtgcgc cgccggcggg tgaaatcacg
     7441 gtagatgaag gggcaactgc cgccattctg gaacgcggca gctccctgtt gccgaaaggc
     7501 attaaaagcg tgactggcaa tttctcgcgt ggtgaagtca tccgcatttg caacctcgaa
     7561 ggtcgcgata tcgcccacgg tgtcagtcgt tacaacagcg atgcattacg ccgtattgcc
     7621 ggacaccact cgcaagaaat tgatgcaata ctgggatatg aatacggccc ggttgccgtt
     7681 caccgtgatg acatgattac ccgttaagga gctggccgat gctggaacaa atgggcattg
     7741 ccgcgaagca agcctcgtat aaattagcgc aactctccag ccgcgaaaaa aatcgcgtgc
     7801 tggaaaaaat cgccgatgaa ctggaagcac aaagcgaaat catcctcaac gctaacgccc
     7861 aggatgttgc tgacgcgcga gccaatggcc ttagcgaagc gatgcttgac cgtctggcac
     7921 tgacgcccgc acggctgaaa ggcattgccg acgatgtacg tcaggtgtgc aacctcgccg
     7981 atccggtggg gcaggtaatc gatggcggcg tactggacag cggcctgcgt cttgagcgtc
     8041 gtcgcgtacc gctgggggtt attggcgtga tttatgaagc gcgcccgaac gtgacggttg
     8101 atgtcgcttc gctgtgcctg aaaaccggta atgcggtgat cctgcgtggt ggtaaagaaa
     8161 cctgtcgcac taacgcggca acggtggcgg tgattcagga cgccctgaaa tcctgtggct
     8221 taccggcggg tgccgtgcag gcgattgata atcctgaccg tgcgctggtc agtgaaatgc
     8281 tgcgtatgga taaatacatc gacatgctga tcccgcgtgg tggcgctggt ttgcataaac
     8341 tgtgccgtga acagtcgaca atcccggtga tcacaggtgg tataggcgta tgccatattt
     8401 acgttgatga aagtgcagag atcgctgaag cattaaaagt gatcgtcaat gcgaaaactc
     8461 agcgtccgag cacatgtaat acggttgaaa cgttgctggt gaataaaaac atcgccgata
     8521 gcttcctgcc cgcattaagc aaacaaatgg cggaaagcgg cgtgacatta cacgcagatg
     8581 cagctgcgct gacacagttg caggcaggcc ctgcgaaggt ggtggcggtt aaagccgaag
     8641 agtatgacga tgagtttctg tcattagatt tgaacgtcaa aatcgtcagc gatcttgacg
     8701 atgccatcgc ccatattcgt gaacacggca cacaacactc cgatgcgatc ctgacccgcg
     8761 atatgcgcaa cgcccagcgt tttgttaacg aagtggattc gtccgctgtt tacgttaacg
     8821 cctctacgcg ttttaccgac ggcggccagt ttggtctggg tgcggaagtg gcggtaagca
     8881 cacaaaaact ccacgcgcgt ggcccaatgg ggctggaagc actgaccact tacaagtgga
     8941 tcggcattgg tgattacacc attcgtgcgt aaataaaacc gggtgatgca aaagtagcca
     9001 tttgattcac aaggccattg acgcatcgcc cggttagttt taaccttgtc caccgtgatt
     9061 cacgttcgtg aacatgtcct ttcagggccg atatagctca gttggtagag cagcgcattc
     9121 gtaatgcgaa ggtcgtaggt tcgactccta ttatcggcac cattaaaatc aataagttac
     9181 acatcattag taccttcctt attttttgac tgggacaaat ttgggaccga tgggttcagg
     9241 atcgagtcta tttgccgtgc gtgttcggta aggtgattag gtgcaaggtg agcatatcga
     9301 cgaaccattt cgatagactc ccagcctccc atttcctgta acactgacaa cgggactccg
     9361 gcttgaacca gccaacttgc ccaggtgtgt ctcaagtcgt gaaatctgaa atcatcaata
     9421 ccagcccgtc tcagcgccgc tttccaggct gtgtttgcgt cataccgcat cttccttact
     9481 gttggcgctt tcgttccgtc tggtttggta cagctttcct tgtacacaaa tacccaacgg
     9541 tgatgattcc cgatttgttt tttcaaaacg cgacatgcag tatcattcag cgcaacgccg
     9601 attgcgcggt ttgatttact ctcttccggg tttatccatg ccacccggcg ctgcatatct
     9661 atttgttgcc attcaaggtt gatgatgttc gagcgtctta agcctgttgc cagtgcaaat
     9721 tcaacaacag actttaatgg ctccggacat tcatcaatca gcctttgtgc ttcatggggc
     9781 tccagccagc ggatccgttt attctttggt tgaggcactt taataattgg tgccttatcc
     9841 agcattttcc attcacgctc tgcggctctt ag
//
LOCUS       DBKEKI010000147         9852 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.148, whole genome shotgun sequence.
ACCESSION   DBKEKI010000147 DBKEKI010000000
VERSION     DBKEKI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.148"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..198)
                     /locus_tag="ACUQPX_003540"
     CDS             complement(<1..198)
                     /locus_tag="ACUQPX_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAT RNA binding domain-containing protein"
                     /protein_id="HHV9058109.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLS"
     gene            complement(485..1210)
                     /gene="phoU"
                     /locus_tag="ACUQPX_003541"
     CDS             complement(485..1210)
                     /gene="phoU"
                     /locus_tag="ACUQPX_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HHV9058110.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(1225..1998)
                     /gene="pstB"
                     /locus_tag="ACUQPX_003542"
     CDS             complement(1225..1998)
                     /gene="pstB"
                     /locus_tag="ACUQPX_003542"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HHV9058111.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(2090..2980)
                     /gene="pstA"
                     /locus_tag="ACUQPX_003543"
     CDS             complement(2090..2980)
                     /gene="pstA"
                     /locus_tag="ACUQPX_003543"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HHV9058112.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2980..3939)
                     /gene="pstC"
                     /locus_tag="ACUQPX_003544"
     CDS             complement(2980..3939)
                     /gene="pstC"
                     /locus_tag="ACUQPX_003544"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HHV9058113.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(4026..5066)
                     /gene="pstS"
                     /locus_tag="ACUQPX_003545"
     CDS             complement(4026..5066)
                     /gene="pstS"
                     /locus_tag="ACUQPX_003545"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HHV9058114.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(5314..6387)
                     /gene="lpfD-O113"
                     /locus_tag="ACUQPX_003546"
     CDS             complement(5314..6387)
                     /gene="lpfD-O113"
                     /locus_tag="ACUQPX_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HHV9058115.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVVLAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(6398..8920)
                     /gene="lpfC-O113"
                     /locus_tag="ACUQPX_003547"
     CDS             complement(6398..8920)
                     /gene="lpfC-O113"
                     /locus_tag="ACUQPX_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HHV9058116.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDMDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(8945..9676)
                     /gene="lpfB-O113"
                     /locus_tag="ACUQPX_003548"
     CDS             complement(8945..9676)
                     /gene="lpfB-O113"
                     /locus_tag="ACUQPX_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HHV9058117.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTAIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(9724..>9852)
                     /locus_tag="ACUQPX_003549"
     CDS             complement(9724..>9852)
                     /locus_tag="ACUQPX_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9058118.1"
                     /translation="YTAIDAEGKAEMKFIACYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         2552 a         2484 c         2238 g         2578 t
ORIGIN      
        1 acttaagagt tcgcttaatc gcccgttcag ttcatgactg ctcaaggcat actctttttc
       61 tattccactt gagttaattc tttcgccagc gcgtttttga aagccaattc cgcgccccat
      121 gacgactttt tcccgctgtt gatcatcaat aaccaccaca acattattgt tgagaatttt
      181 ggtgatttgc atgttcatag caaggacctt ttttataaac aaaaaaaacc cgacttcacc
      241 agtattctct ggttatgtca ggttttgcct gcgaatgcag taacaatcca gtcatttatt
      301 aatggttttt ataacgaaca tccaggttcg gaaattaatt taattgcgtg cagagaaaat
      361 agcaatgcgc tattgataaa aatatgacca tgctcgcagt tattaacttt gtgtaatttt
      421 aggaatttat aaagttatat ataacaaatc ccaataatta agttattggg atttgtctgg
      481 tgaattattt gtcgctatct tttcccgcca gcagtttatc cagctcatca ccgcctacat
      541 gacggaaatc ctgacccttc acgtagtaga agataaactc acaaatattc tggcagcggt
      601 cgccgatacg ttcgattgaa cgcgcacaga acagcgcggt aagtacgctc ggaatagtgc
      661 gcgaatcttc catcatgtag gtcatcagtt gacgaacaat accttcgtat tcctgatcga
      721 cttttttatc ttcacgataa atacgtaccg cttcgtcaat gtccatccgc gcgaacgcgt
      781 ccagcacgtc gtgcagcatc tggatggtat gacggcccag cgactccaga cttaccagca
      841 acggctgatg ctgctgggag aatttctcca gcgcagtacg gcagattttg tccgccacgt
      901 cgccaatacg ctccagctcg gcaatggttt tactgatcac cataaccagt cgcaggtcgc
      961 tcgccgtcgg ctgacgtttg gcgataatgc gcacgcaggc ttcatcgatc gccacttcca
     1021 tcatgttgac gttcttgtcg ccttcgatca cgcgcttcgc cagatcgctg tcctggttat
     1081 gcatcgcggt gattgcatca gaaagctgct gctccaccat gccgcccatg gtcatcacct
     1141 gcgtgcggat actttccagt tcggcgttga actggccgga aatatgttta ttaagattga
     1201 gactgtccat aacgcactcc tgaatcaacc gtaacgaccg gtgatgtagt cttctgtttg
     1261 tttcttcgct ggcttggtga acagatcgtc cgtgttgctg aactcaatca attcgcccaa
     1321 gtacataaat gccgtgtggt cggaacaacg cgcagcctgc tgcatgttgt gggtgacgat
     1381 caccacggtg taatcctgct tcagttcggt gatcagctct tcaatacgcc cggtagagat
     1441 ggggtcgagc gccgaacacg gttcgtcgag cagcagcact tccgggcgaa tggcgatacc
     1501 acgcgcaata cacagacgct gttgctgacc accagagaga gagtaaccgc tctggtgcaa
     1561 tttatctttg gtttcgttcc acaatgcggc tttggtcaat gcccactgca cgcgctcgtc
     1621 catgtcggca cgggagagct tctcaaacag acgaacgcca aaggcgatgt tgtcgtagat
     1681 ggacatcggg aacggcgtcg gtttctggaa caccatgccc actttcgcac gcagcagggc
     1741 gatatcctga gagttggtca ggatgttgtc gccatcaagc agaatttcac cttccgcacg
     1801 ctgctccggg tacagttcaa acattttgtt gaaggtacgc agcagcgtcg atttaccgca
     1861 gccggacggc ccgataaacg ccgtaacctg gtttttggcg atatccaggt tgatgttttt
     1921 cagggcatgg aatttgccgt agtagaagtt caaattacga acctgaattt tactcggggc
     1981 agtttcaacc atactcattg caatcttttc ctcatcgtac ctgatgcttt tcgctaatca
     2041 ggcttaccgt agggccgggt aaggcgttca cgccgcatcc ggcaaagtgt taaccgtgtt
     2101 tattcttcgc aaaaacaacg cgcgccagaa tgttcagcag caggacgcac agggtaatga
     2161 tcaatacccc ggcccaggcc agttgttgcc attccgcaaa cgggctcatc gcaaacttaa
     2221 agatcgtcac cggcaggttg gcgatcggct gcatcatgtc cgtgctccag aactggttgg
     2281 agagcgcggt aaacagcagc ggcgcggttt caccggcaat acgggcaatc gccagcagga
     2341 taccggtcat aatcccggac accgacgctt tcagcgtaat cgcggagatc atcttccact
     2401 tcggtgtacc cagcgcataa gccgcttcac gcaggctgtc cggcaccagt ttcagcatgt
     2461 tctcggtggt gcggataacg atcggcacct gcaacagcgc cagcgcaatt acgcccgccc
     2521 agccggagaa gtgctccatc tgtgccacca cgatggtgta gacaaacaga ccaaccacaa
     2581 tcgacggcgc ggagagcaga atgtcgttaa tgaagcgaat cacttctgcc agccaggatt
     2641 tacgaccata ttccgccaga taaatccccg ccataatgcc cagcggcgta ccgaataccg
     2701 tggcccacaa aattaacagc ccgctacccg ccagggcgtt cgccagacca ccaccttccg
     2761 tattaggcgg cggcgtcatt tcagtgaaca gcgccagcga cataccgtcg ataccgcgag
     2821 tgatggtgga cattaaaatc cagatcagcc agaacaggcc gaaggccatc gtcgccatcg
     2881 agagcgtcag cgcaatacgg tttttgaggc ggcgacgcgc ctgcattttg cggcgagatt
     2941 cagccagcgc cgcagtggtt tgcatttcaa ccatagccat tagcgtgccc cctcattctt
     3001 agccaggcgc ataatcataa acttcgatgc ggcgaggacg ataaaggtaa tcacaaacag
     3061 gatcaggccc agttccatca gtgcggcaac gtgcagaccg gattccgctt ccgcaaattc
     3121 gttcgccagc gcagaggtga tactattgcc cggcatgtac agcgaggcgc tgtcgagctg
     3181 gtaggtgtta ccgatgataa aggtcaccgc catggtttca ccgagcgcgc ggcccagccc
     3241 cagcatgatg ccgccgataa caccattttt ggtgaacgga agaacgatac gccagataac
     3301 ttcccaggtg gtgcagccaa taccgtaggc cgactctttc atcatcaccg gggtttgttc
     3361 gaacacatca cgcattaccg ccgcaatgta cggaataatc atgatggcga ggatcacgcc
     3421 tgccgcgagg ataccgatac caaatgcggg gccagagaac agcgcgccaa caatcgggat
     3481 attcgacatg atattgccga ccggctcctg aaagtaaacg gcgaacagcg gcgcaaagat
     3541 aaacaggccc cacatgccgt aaacgatact tggaatggct gccagcagct caatggcgat
     3601 acccagcggg cgtttcagcc agccaggcgc aagctcagtc aggaacaggg cgataccgaa
     3661 actcaccggg acggcgatca gcagcgcgat aaacgaagtc accaacgtac cgtagatcgg
     3721 caccagcgcc ccgtagatat cgttcggtgc atcccactct ttggtccata ggaaagccag
     3781 accaaatttc tgaatgctcg gccaggagga gatgatcaga gagacaataa tgccacccaa
     3841 catcaatagc acaatcagcg ccgccagttt taccagcacg ctgaaaatta tgtcgccctt
     3901 tttacccggt gggttaaaag cgggcttggt tgcagccata agttactctt cagttaaacg
     3961 cgtttacaaa gtcattttgt aatgccggat gcggcgtaaa acaccgtacc cggcctggag
     4021 ttttattagt acagcggctt accgctactg tctttaatat tggtcttcca cgcagcgcga
     4081 acctgttcaa ctacactatc cggcaggctg gcgtaatcca ggtcgttcgc ctgtttagcc
     4141 ccggttttgt acgcccagtc gaagaatttc agcacctctg tgccttgttc tggtttcttc
     4201 tgatctttgt ggatcagaat gaacgtggta gaggtaatag gccatgcatc ttcgcctttc
     4261 tggttggtca gatcctgagc gaaggttttg ctccagtctg caccttttgc tgcattagcg
     4321 aagttttctt cggtcggact aaccggttta ccatcagcgg agatcagttt ggtgtacgcc
     4381 aggttgttct gcttcgcgta agcatattca acataaccaa ttgcacccgg cagacgctga
     4441 acgaacgcgg cgataccgtc gttaccttta ccgcccagac cgatcggcca ttttacggta
     4501 gagccagtac caacgttgtt tttccactct tcgttcactt tcgccaggta gctggtgaag
     4561 acgaaggaag tcccggagcc atctgcgcgg cgtactacag caatgttttg tgaaggcagt
     4621 ttcagacccg gattcagttt ggcgatggct tcatcatccc acttcttgat tttgcccagg
     4681 tagatgtcgc cgagggtttt accatccagc accagttcgc cagacttcag ccctggaatg
     4741 ttaaccgcca gcaccacgcc gccaatcacg gtcgggaact ggaacagacc ttcctgagcc
     4801 agtttttcgt cagacagcgg cgcgtcagag gcaccaaaat caacggtatt agcgataatc
     4861 tgttttacgc caccggaaga accgataccc tggtagttaa ctttattacc ggtttctttc
     4921 tggtaagtgt cagcccattt ggcatacacc ggcgcaggga aggttgcacc tgcacctgtc
     4981 aggcttgctt ctgcaaacac agagaaagca ctcatcgata aggtcgcggc gacaacagtt
     5041 gcgacggtgg tacgcataac tttcataatg tctcctggga ggattcataa agcattgttt
     5101 gttggctacg agaagcaaaa taggacaaac aggtgacagt tatatataag gaatatgaca
     5161 tttttatgac agaaaggaat taccaaataa cttactgatt ttaaatgtga agatgaaaat
     5221 cccacatgcc atctttttgt catgatgcat ttaaaagtga taaagaaatg ggtgagatga
     5281 caacgatgtg atgaacaatc gttgtcagac ctattagcgc acaatctcaa cagttatcgt
     5341 cgctgttgca gtaaattgcc ccggggctgg gcgagctcca gtaaggctcg caggagcagc
     5401 tgaaaaagtt acactacctg atttttcacc atacactgtt gatttaccca tgtctgttgg
     5461 caactcacca ccgttcactt tgatttcctt attatttttg tcgtaaattt ttatccccac
     5521 gttagagttt gaagttacga ccattgaatt gtccgtattg ggttcggtag cataaaaaga
     5581 cgtactgata tcatcggtat tcagtatccc caggttttca cactcaatag tgactgtttt
     5641 agtaatttta tgtgttgtta ccgcacttcc tacaacagat gaaaaatctg cagctggtat
     5701 ttctttaaaa tcaaaacaaa ctggctttaa attatttatt tcgcaacttt gcggagctgt
     5761 aatatcgccc cgaatataaa catcagccat cggctgtgag taatccaccg gggtgtcacg
     5821 gctaattgtt ccatacaatg tcactatctt tgtttcagga ataatcaatt ttcctgcaaa
     5881 tgtcttcttg atataaaaat atactatcgc tttagcccca ctggatatac ttaaaggttc
     5941 aatgcggttg caattgtact ttgtattacc agacgggttg ttagcatgat attcaaaagg
     6001 tgcattcaca tacccaaccc caagaatatc tatagcaatc ccaacatcaa ggttcggatt
     6061 aagatcataa taattaatat tagagtgaac cccagcataa gttaatgcct ggtttatttt
     6121 tgacgtataa tagatatagg ggaattctcc tacgttacca gtatcacaat gacagcgtcc
     6181 aagatatgag gatccattag ttacagtggt gttaaatgtc gttccaacct tattttcgtt
     6241 cgcggtaata agatatcccg agaaatcaag cgtactgtga aatacgccat tatcaggggt
     6301 acagatacct ttaggagctg caagtactac actacttaac gagagcataa acacagcaaa
     6361 gcaccactgt ttgatatact tgttcataaa aatcctttta atgacatgtc acaccctgtt
     6421 caattaatcc gctctcactt tctggtgtcg aaagtgaata attgaagatg cattttgttg
     6481 aggcgttttc tccccaggat aacgtaacct gtcctttttc tggtagacca gaaagataaa
     6541 gttcgccacg gtcaccgaca attcctgaat ctgccaaact tgcatcttgt atcgcagcaa
     6601 tggctccaaa aggtaccggt ttaccatttg catttaacac tctgaagaga acgcgataac
     6661 ccacatgagt cacaaactcc gctttaacca ctgcaccacg cgtgggaacc atcgtaacga
     6721 cggtattatc aagttctata ttcgacttcg agaaatagtt atcgctgatt gctacccgat
     6781 tttcatcata agggtttagc tgtgtcttga ccgtatagcc gcgccagtcc gtcgcagcac
     6841 cgcgcatatt atctatttct aacccggcgg ccccaggtgc tttaacaaga gcaatagttt
     6901 cacctaactc ctgacttaac gttacccctt cgctgtgtgc cagaattccc cctctgatac
     6961 cataattcag cctctgggaa ttttggctgt agttatagct gccgttcacc gtgccataag
     7021 agccatcata ggtcagggac atattaccgc caacctcatc atcatgactg ttataggact
     7081 gcattaaatt ccatgaaagg ttatttcttt ccagcgccgt accgctgagt ccgagttgat
     7141 gtgagctacc ggaattatcg ttttgcgaaa cagaataggt ggcgtaacta ttaccccacc
     7201 agcccgataa aggaacagat aaagagagca tgtagatctg ctcactggtg ttatcactgg
     7261 catggttgtt ccccgtcccc tgtctggcag tattggaata actccaggag acgttccacg
     7321 agagacgctt ccagctactg ctgtagccaa attgcattaa tcgttcgaca tcatcggtat
     7381 gccagtattg ttgccggctt aatgtcaggt ataaattgcc ataatctctc cccaacgact
     7441 gcgttagcgt tccctccacg cgactacgtc ggttacctgt ttcccaaaag tcttcagggc
     7501 tgttgcgccg cgatgcccac tcattgaggg tatagtagcc ctcggtggag taacgatagc
     7561 caaccaggcg caagctagtg tctgtgtcgt caaatagttt tgaatagaga aagcgatatg
     7621 actgacccgt ttctgtatca tcatggctaa aattcgcacg agcatgtgtc acatcgaaag
     7681 aaattgcccc caaagcgcca agatctttac cgacacccag agctgcagaa ccataatttt
     7741 cagcagcctg catacctcca tataaagtga tcccgtggga aaaaccatga agtatttgcg
     7801 cctgaaggac gtccggtgta aatccgtctt catcgcgcaa ttcacccacg ctgacatcaa
     7861 catctgtctg accttcacgt ttaagaatcg ccaatgaagc ataaggttgg gtgaatcgtt
     7921 gttcgctacc gtcttcttct ttgatggtta cgtccaaatc gccacctgta cttgcggtgt
     7981 tgagatcggt gatctcaaaa gcaccttgtg gaacggctga ctgataaatc acgtagccat
     8041 tctgccgaat gatgacggtg gcgttggact ttgcaatccc acgaaccaca ggagcaaagc
     8101 cattctggct ggcgggtagc atatcgttat cagtatacaa acgcgcgccg cgaatttgcg
     8161 tactgtcgaa aatatcgctc gccgtccacg tatcaccaat cataatctgg cttcttaacg
     8221 cggcaatatc gcgctgtatc caggtactaa tggagttgta ttccgcacgg ccatcgctgg
     8281 tgtttaaagt actgtaatta cgtagccgcc acggtccaat attcagtcca ttgcgcagat
     8341 tgagataact ggtatctgtc tcttgtgaac cataatctga acgtagtgta tgtgaaccag
     8401 aaaaatcgta gttgatggtg agcgcattaa tcccctcatc ccaacgcgtg gggctgatgt
     8461 aatcataagc catcgcgtcc atgtagattt ggtgaatgga tatattcagc tgcaatttat
     8521 cactgtcaaa ctgccaggat gagccttcaa tacgtggagc aggatcgagg caaaccgtct
     8581 catccatatc attgaacgat ttgagcgcat caacttttat gccaaaggag gacagtgttt
     8641 ttttaatggc aataaagcaa gcgtgcagat cagcatctgg attatcctga acaaatgtca
     8701 catcctgacg ttcaaacatt ttaccgttga taaaaatatc aacgctataa acacctgggg
     8761 gcatcaggtt tgcccgatta aactggcgaa tatcgactcc ggattgatgg tcgatttcca
     8821 gaagcgccgg attgaataca ctgacctcgg ccatcaggtt ttgcgagaaa atcagacacg
     8881 tccctgccgt gaggccaacc actattctgg tcgtcatcat aatacgccta tccttatccc
     8941 ttgcttacag cgtcattttt atcgggtcac tgactgcgcc gtaatcatta ataaatcgcc
     9001 aggtcatttg gcctcccgca gaattagcag gtaaaggtaa cgtgagcgtt tcaaaaggac
     9061 ggatcatatt gaggggcaca tcatggccat caacgatgac atccatcaca ctgatatgaa
     9121 ttgggttggg attctctgct atcagatttc gcccttcccg gcgccatttc actttcggcg
     9181 cagcatcttc gggaataagg cggatatttg ctggtcgcca gaacagcttg atacgcgttt
     9241 ttactgctat ttccagcgag ttcgtcgctt tgttgctcgc tgatggcgga atatttttaa
     9301 tattcagcca gaagactgac tcgcgatcat caggcaatga cacacctttg gtgtgaataa
     9361 tgcgtaatat tttttcttgc gaaggttcta atcgtgatac tggcggaata gcggtaaacg
     9421 gcgctttgct tttatcctct ttattaaatg gatctaccca agcctggatg agataaggta
     9481 ttttttcttc tttgctaaat acagaaatag atttatcagc attattactt aagtaaacca
     9541 cccgcgtacc accaatgaca acgcctgcat aagttaatgc tggttggcct aaaaaaacgc
     9601 tgagtataat cgcggtaaat aacttacatg aaactgttgt tttaaagagc ctgattaaac
     9661 aactttttct ggacataacg actccctggc caaagccaac tgaataaaaa ggcccttgat
     9721 aaattatttc ttatattcga cagtaaattg tgaatcagcg ttcgccgtac cagccgttac
     9781 ggtcgcattg gttgccacat agcatgcaat aaacttcatt tcagccttgc cttcagcatc
     9841 gatagcggta ta
//
LOCUS       DBKEKI010000148         9782 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.149, whole genome shotgun sequence.
ACCESSION   DBKEKI010000148 DBKEKI010000000
VERSION     DBKEKI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.149"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..324
                     /locus_tag="ACUQPX_003550"
     CDS             <1..324
                     /locus_tag="ACUQPX_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HHV9058119.1"
                     /translation="LNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDN
                     AVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQT
                     YMPRV"
     gene            421..573
                     /gene="hokE"
                     /locus_tag="ACUQPX_003551"
     CDS             421..573
                     /gene="hokE"
                     /locus_tag="ACUQPX_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HHV9058120.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            924..1076
                     /gene="hokE"
                     /locus_tag="ACUQPX_003552"
     CDS             924..1076
                     /gene="hokE"
                     /locus_tag="ACUQPX_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HHV9058121.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1198..1818)
                     /gene="entD"
                     /locus_tag="ACUQPX_003553"
     CDS             complement(1198..1818)
                     /gene="entD"
                     /locus_tag="ACUQPX_003553"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HHV9058122.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1975..4215)
                     /gene="fepA"
                     /locus_tag="ACUQPX_003554"
     CDS             complement(1975..4215)
                     /gene="fepA"
                     /locus_tag="ACUQPX_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HHV9058123.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADARDINQGHQFARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASTGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4458..5660
                     /gene="fes"
                     /locus_tag="ACUQPX_003555"
     CDS             4458..5660
                     /gene="fes"
                     /locus_tag="ACUQPX_003555"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HHV9058124.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5663..5881
                     /gene="ybdZ"
                     /locus_tag="ACUQPX_003556"
     CDS             5663..5881
                     /gene="ybdZ"
                     /locus_tag="ACUQPX_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HHV9058125.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5878..9759
                     /gene="entF"
                     /locus_tag="ACUQPX_003557"
     CDS             5878..9759
                     /gene="entF"
                     /locus_tag="ACUQPX_003557"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HHV9058126.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQWYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGCSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
BASE COUNT         2161 a         2539 c         2679 g         2403 t
ORIGIN      
        1 cttaatcctc acgagcatcc tgttctgcac tctgaccagg gatggcagta tcgtatgaga
       61 agatatcaaa atatccttaa agaacatggt attaaacaaa gcatgtccag aaaaggcaat
      121 tgtctggata atgctgtggt ggagtgtttc tttggaacct taaagtcgga gtgtttttat
      181 cttgatgagt tcagtaatat aagcgaactg aaggatgctg ttacggaata tattgaatac
      241 tataacagca gaagaattag cctgaaatta aaaggtctga ctccaattga atatcggaat
      301 cagacctata tgcctcgtgt ataactgtcc aactttttgg ggtcagtaca ccctggggct
      361 gccactccaa accagacaat ttggatggta gtcccttctt cgcatggagg caatataaac
      421 atgctgacga aatatgccct tgtggcagtc atagtgctgt gtttgacggt actgggattt
      481 acgcttctgg tcggagactc gctgtgtgag tttacggtga aggaacgtaa tattgagttc
      541 agggctgttc tcgcttacga accgaagaag tagccgttgt gcggggagta atccccgcat
      601 atccggttgt caggtcagga tggtaaggca cctgctttac actttcgccc gcggtcagtg
      661 atggctgcgg gcgatttcgt tttaagagca atgatgagtt ttgccgacag aagtccgaaa
      721 caggactcac tgtataaata aacagctatt ttgctaagga agggtaagat aacggcgggt
      781 gcctgaagct ttccggtttc aggttaactc tgaggtttgg aaagatgaag ccccaagaga
      841 tatttctatc aatcctgggg ctgccactcc gaacctcaac aactcggatg gtagtccctt
      901 cttcgcatgg aggcaatata aacatgctga cgaaatatgc ccttgtggca gtcatagtgc
      961 tgtgtttgac ggtgctggga tttacgcttc tggtcggaga ctcgctgtgt gagttcaccg
     1021 taaaggaacg taatattgag tttaaggctg ttctcgctta cgaaccgaag aagtagccgt
     1081 tgtgcgggga gtaatccccg catatccggt tgtcaggtca gagtggtaag gcacctgctt
     1141 tacactttcg cccgcagtca gtgatggctg cgggcgaatc gtaccagatg ttgtcaatta
     1201 atcgtgttgg cacagagtta tgactatttt ttcttttatc tgccagtgca cagcaaacat
     1261 ctcattctca cgatgaatga tgacctgctg tttattccag ctaattatct gatagtccag
     1321 aaaacctgca tcagtttgga tctcacttgc cttaaatgcg ctctctttgg cggaaaatgc
     1381 cagtgtcagc gccagagaaa aggctaaacc gcagtctgcg agtcgctcgt gttctgctgg
     1441 tgtaataatg ttgtctgtca attcggtcgc tgtttgcgca gaaaaaattt cttcgatatc
     1501 aacgccaatc ggttgacggg aaaccacggc caatgccgta gccccgcagt ggctaatact
     1561 gccgtatacc tccgcaggcc acaccggttg gcgtagctcg ccgattgcgg gcacacattt
     1621 atagccatat tcccgcaagg cataaacagc agcgatccgt ccggctaaat gctctgtttt
     1681 acgtttacgt ccagcgtgtt gcagttgtgc gtagtgcggc agccagagta aatcctgctc
     1741 acaaaaattc gccggatcga actcaacaaa atgcagcgta tgtccggcaa aggggaggga
     1801 ggtatgcgta gttttcatat cgaccatatt cgagactgat gacaaacgca aaactgcctg
     1861 atgcgctacg cttatcaggc ctacgtgatc ctgcaatata ttgaattggc aagatctttg
     1921 taggccgcat ccggcgtgaa cgacgcgcac tttgtcaaca atctgacgtt agcatcagaa
     1981 gtgagtatta atgctcatat accacgtacg tcccggctcg ttataggtat acgcgccggc
     2041 accggcgata tagttggctc ctgccagatc gcccgtggtc tgggcattac ctgcacgcca
     2101 caaacgtttg tcgaacaggt tgtccacgcc gccggtcaga ctgacattct tcgtcacatc
     2161 ccaggtcgcg ctcagaccca caatgctgta aggactgatt tctttggttt ccgggccaac
     2221 cgctggctga cctttatagt tgtacttctt cggctgctgc ttgccgtacc aggtgaaggt
     2281 cgtttgcatc gacaaatctt cccgtgcctg ccagctcagc gttgagttca acgtgtactc
     2341 cgggatgatc gacaaacggt cgcccgtggt tttgttttca ctcttcagca tataagtgat
     2401 gttattggtc cacatcaccg tttcgctaac tggtacgttt aacgatcctt ccagaccttc
     2461 aaccactgct ttcggcacgt tatcccactg atagagatca gtgccgactg cgttttgccc
     2521 tacagccaca tagcccgctt caatcttatt gcgataatcg ttacggaacc aggtgacgcc
     2581 cgccagccag ccgtcgcgtt tgaactccag accaatctct ttgttgatgc tggtttctgc
     2641 tttcaggtca tcgttacctt gcagatagca gccgcccgtg ctggcatagc agccctgacc
     2701 tttactgtag agaatgtagt tcgggttagt ctggtacagg ctcggcgctt tataggcgcg
     2761 ggcaatgcct attttcagcg tgaagtcatc gcctaaacct tgcgatatgt tcagcgccgg
     2821 gctccagtta ttgccgacaa tactgtgatg atcgaaacgc agccccggcg ttacgatggt
     2881 gctgtcagtc agctccatgt tgttttcggc aaacagcgag aaaatttctg cttttgaata
     2941 cgggctacgg tcggtggcac tcacgccatc aatagcgcca ccggtattcg ttccggtcag
     3001 tgcctgggtg ttggaactta agtccttcat ccgttgctga ttccactccg tacccagcgt
     3061 cagcgtctgg ttaacgagga aatcaatcgg caggttaact tcgctgtgca gcatcacgtc
     3121 atcgagatcg atatcgacga aatcctgtgt cgctttttcg ttaaatttcc cttcggtacc
     3181 gcccgccaga ccttccggaa tacgcgagtt acgggtgtgt tcatactgca cccagttgct
     3241 ggtggtcacg ccgttatccc agccaccgtt ccaggtcagc gcgtagttct ggcgatacag
     3301 gcggttggtt tcatcgccat atttcgagcg agtgtaagcg tcagagttgg tgttctgggt
     3361 atcgcccgca tacaggttac cctggcggct gtaacccgct tccagttcca gcgattgcaa
     3421 tggtgcgaag tcccagcgca ccacgccgtt aatatcttta ttgattaccc cttcgcgccc
     3481 ggctggtaac gtcgtggcat acgttccggc acgcgcgaac tgatggccct ggttgatatc
     3541 cctcgcgtca gcctgagttt tgtcgaggtt gccatacaag cggaagctga attcgtcgcc
     3601 cagcggaccg gtcaggctaa agttagtgcg tttggtggca ccttcctctt tatgttctgg
     3661 cgcattgaaa tatgcgtccc aagagccgtg ccactcgccg ctgccttttt tggtaatgat
     3721 gttaaccacg ccgcccgccg cgccgttgcc ataacgcgca gctgccggac cacgcagaac
     3781 ttcaatacgt tcaatcattt caggtggcac ccaggaggta tcaccacggg tatcgcgctc
     3841 gccacgccag ccctgacgca ccgagttacg gctgcttacc ggcttgccgt caatcaaaat
     3901 cagcgtgttt tccggaccca taccgcgaat atcaatctgt cggttattcc cacgctgacc
     3961 actggtggag ttaccggtca ggttaacgcc tggcatggta cggatgatct ccgacacatc
     4021 gcgggcaacc gggtttttgc ggatttcatc tgcggtgatg gtcgaaacgc caggtgcctg
     4081 caagttctgc tcggcggcgg taacgacaat ggtatcgtca tgtgaaacag gagtatcggt
     4141 cggctcttgt gcctgcgcta ccccataaat ccccagattg accaacaagg ccagggaatg
     4201 aatcttcttg ttcattgttt tattcctgca tttttaccac gaattgcaac tgtcgggcat
     4261 ggtcgtcatc aacacgacgc atcccgttac cgcgaaaacc tttgatcctg aaagacacgc
     4321 agtgcagttg gttaattaat gtccgcgctt cccacggcgc gccattacgc tattgtaaat
     4381 gcaaatagtt atcaataata ttatcaatat atttctgcaa tcaatgaaaa attgcaaagt
     4441 aaacatgggg ttatggtgtg acggcgttaa aagtaggaag tgagagctgg tggcagtcga
     4501 aacatggccc ggaatggcag cgtctgaatg acgaaatgtt tgaggtcact ttctggtggc
     4561 gtgatcccca aggttctgaa gaatactcaa cgataaagcg cgtatgggtc tacatcactg
     4621 gtgtgaccga tcatcatcag aacagccagc cccagtcgat gcagcgaatt gcaggtactg
     4681 atgtctggca gtggacgaca caactcaatg ccaactggcg cggcagctac tgctttattc
     4741 ccaccgaacg cgatgacatt ttttctgcac catcccccga tcgcctcgaa ttacgtgaag
     4801 gctggcgaaa actattaccc caggcaatag ccgatccgct gaatccacaa agctggaaag
     4861 gcgggcgagg gcacgctgtt tctgcactcg aaatgccgca agcgcctctg caaccgggat
     4921 gggattgtcc gcaagcgcca gaaataccag ccaaagaaat tatctggaaa agtgaacggt
     4981 tgaaaaattc acgtcgtgta tggattttta ccaccggcga tgtaacagca gaagaacgcc
     5041 cgctggcagt tttgctcgat ggcgaatttt gggcgcaaag tatgcccgtc tggcctgtgc
     5101 tgacttcgct gacccatcgt cagcaacttc ctcccgccgt gtatgtgttg atcgacgcta
     5161 tcgacaccac gcaccgcgcc cacgaactgc cgtgtaatgc ggatttctgg ctggcagtac
     5221 agcaagagtt attacccctg gtgaaagtta ttgccccttt tagcgatcgc gccgatcgca
     5281 ccgtggtcgc cgggcagagt tttggtgggc tgtccgcgct gtatgccgga ctgcactggc
     5341 ctgaacgctt tggctgtgta ttaagccagt caggatcgta ctggtggccg catcggggcg
     5401 ggcagcaaga gggcgtgtta cttgaaaagc taaaagctgg tgaagttagc gccgaaggtc
     5461 tgcgcattgt gctggaagcg ggtattcgcg agccgatgat catgcgggcc aatcaggcgc
     5521 tgtatgcgca attacaccct ataaaagaat ccattttctg gcgtcaggtt gacggcggac
     5581 atgatgcgct ttgttggcgc ggtggcttga tgcaggggtt aatcgacctc tggcaaccac
     5641 ttttccatga caggagttga atatggcatt cagtaatccc ttcgatgatc cacagggagc
     5701 gttttacatt ttgcgcaatg cgcaggggca attcagtctg tggccgcaac aatgcgcctt
     5761 accggcaggc tgggacattg tgtgtcagcc gcagtcacaa gagtcctgcc agcagtggct
     5821 tgaagcccac tggcgtactc tgacaccggc gaatttcacc cagttgcagg aggcacaatg
     5881 agccagcatt tacctttggt cgccgcacaa cccggcatct ggatggcaga aaaactgtcg
     5941 gatttaccct ccgcctggag cgtggcgcat tacgttgagt taaccggaga ggttgatgcg
     6001 ccattactgg cccgcgcggt ggttgccgga ttagcgcaag cagatacttt gcggatgcgt
     6061 tttacggaag ataacggcga agtctggcaa tgggtcgatg atgcgctgat attcgaactg
     6121 ccagaaatta tcgacctgcg aaccaatatt gatccgcacg gtactgcgca ggcattaatg
     6181 ctggcggatt tgcaacaaga tttgcgcgtc gatagcggta aaccactggt ctttcatcaa
     6241 ctgatacagg tggcagataa ccgctggtac tggtatcagc gttatcacca tttactggtc
     6301 gatggcttca gttttccggc cattacccgc cagatcgcca acatttactg cgcattgctg
     6361 cgtggcgaac caacgcctgc ttcgccgttt acgcctttcg ctgatgtagt ggaagagtac
     6421 cagcaatacc gcgaaagcga agcctggcag cgtgatgcgg cattctgggt ggaacagcgt
     6481 cgccaactgc caccgcccgc gtcactttct ccggcacctt tagcggggcg cagcgcctcg
     6541 gcagatattt tgcgtctgaa aatggaattt accgacgggg aattccgcca gctggctacg
     6601 caactttcag gtgtgcagcg taccgattta gcccttgcgc tgacggcttt gtggctgggg
     6661 cgattgtgca atcgcatgga ctacgccgcc ggatttatct ttatgcgtcg actgggctcg
     6721 gcggcgctga cggctaccgg acccgtgctc aacgttttgc cgctgggtat tcatattgcg
     6781 gcgcaagaaa cgctgccgga actggcaacc cgactggcag cacaactgaa aaaaatgcgt
     6841 cgtcatcaac gttacgatgc cgaacaaatt gtccgtgaca gcgggcgagc ggcaggtgat
     6901 gaaccgctgt ttggtccggt actcaatatt aaggtatttg attaccaact ggatattcct
     6961 ggtgttcagg cgcaaaccca taccctggca accggtccgg ttaatgacct tgaactggcc
     7021 ctgttcccgg atgaacacgg tgatttgagt attgagatcc tcgccaataa acagcgttac
     7081 gatgagccaa cgttaatcca gcatgctgaa cgcctgaaaa tgctgattgc ccagttcgcc
     7141 gcggatccgg cgctgttgtg cggcgatgtc gatattatgc tgccaggtga gtatgcgcag
     7201 ctggcgcaga tcaacgccac tcaggttgag attccagaaa ccacgctgag cgcgctggtg
     7261 gcagaacaag cggcaaaaac accggatgct ccggcgctgg cagatgcgcg ttacctgttc
     7321 agctatcggg aaatgcgcga gcaggtggtg gcgctggcga atctgctgcg tgagcgcggc
     7381 gttaaaccag gggacagcgt ggcggtggca ctaccgcgct cggtcttttt gaccctggca
     7441 ctccatgcaa tagttgaagc tggcgcggcc tggctaccgc tggataccgg ctatccggac
     7501 gatcgcctga aaatgatgct ggaagatgcg cgtccgtcgc tgttaattac caccgacgat
     7561 caactgccgc gctttagcga tgttcccaat ttaaccaacc tttgctataa cgccccgctt
     7621 accccccagg gcagtgcgcc gctgcaactt tcacaaccgc accacacggc ttatatcatc
     7681 tttacctctg gctccaccgg caggccgaaa ggggtaatgg tcgggcagac ggctatcgtc
     7741 aaccgcctgt tgtggatgca aaatcattat ccgctcacag gcgaagatgt cgttgcccaa
     7801 aaaacgccgt gcagttttga tgtctcggtg tgggagtttt tctggccgtt tattgccggg
     7861 gctaaactgg tgatggctga accggaagcg caccgcgacc cgctcgctat gcagcaattc
     7921 tttgccgaat atggcgtaac gaccacgcac tttgtgccgt cgatgctggc agcatttgtt
     7981 gcctcgctga ctccgcaaac cgctcgccag agttgcgtga cgttgaaaca ggttttctgt
     8041 agtggtgagg ccttaccggc tgatttatgc cgcgaatggc aacagttaac cggcgcgccg
     8101 ttgcataacc tgtatggccc gacggaagcg gcggtagatg tcagctggta tccggctttt
     8161 ggcgaggaac tggcacaggt gcgcggcagc agtgtgccga ttggttatcc ggtatggaat
     8221 acgggtctgc gtattcttga tgcgatgatg catccggtgc cgccgggtgt ggcgggtgat
     8281 ctctatctca ccggtattca actggcgcag tggtatcttg gacgacccga tctgaccgcc
     8341 agccgcttta ttgccgatcc ttttgctcct ggtgaacgga tgtatcgtac cggagacgtt
     8401 gcccgctggc tggataacgg cgcggtggag tacctcgggc gcagtgacga tcagctaaaa
     8461 attcgcgggc agcgtatcga actgggcgaa atcgatcgcg tgatgcaggc gctgccggat
     8521 gtcaaacaag ccgttaccca cgcctgtgtg attaaccagg cggcagccac tggtggtgat
     8581 gcgcgtcagt tggtgggcta tctggtatcg caatcgggcc tgccgttgga taccagcgca
     8641 ttgcaggcgc agcttcgcga aacattgccg ccgcatatgg tgccagtcgt tctgctgcaa
     8701 ctgccgcagt tgccacttag cgccaacggc aagctggatc gcaaagcctt accgttgcct
     8761 gaactgaagg cacaagcgcc agggcgtgcg ccgaaagcgg gcagtgaaac gattatcgcc
     8821 gcggcattcg cgtcgttgct gggttgtgac gtgcaggatg ccgatgctga tttctttgcg
     8881 ctgggcggtc attcgctact ggcaatgaaa ctggcagcgc agttaagtcg acagtttgct
     8941 cgtcaggtga cgccggggca ggtgatggtc gcgtcaaccg tcgccaaact ggcaacgatt
     9001 attgatggtg aagaggacag ctcccggcgc atgggattcg aaaccattct gccgttgcgt
     9061 gaaggtaatg gcccgacgct gttttgtttc catccggcgt ccggttttgc ctggcagttt
     9121 agcgtgctct cgcgttatct cgatccacaa tggtcgatta ttggcattca gtcgccgcgc
     9181 cctcatggac ccatgcagac agcgacaaac ctggatgaag tctgcgaagc gcatctggca
     9241 acgctacttg agcaacaacc gcacggccct tattacctgc tgggttgttc acttggcggt
     9301 acgctggcgc agggcattgc ggcgcggctg cgtgcccgtg gcgaacaggt ggcatttctt
     9361 ggtttgctgg atacctggcc gccagagacg caaaactggc aggaaaaaga ggctaatggt
     9421 ctggacccgg aagtgctggc ggagattaac cgcgaacgcg aggccttcct ggcggcacag
     9481 cagggaagta cttcaacgga gttgtttacc accattgaag gcaactacgc tgatgctgtg
     9541 cgcctgctga cgactgctca tagcgtaccg tttgatggta aagcgacgct gtttgttgct
     9601 gaacgcacgc ttcaggaagg tatgagcccc gaacgcgctt ggtcgccgtg gattgcggag
     9661 ctggatatct atcgtcagga ttgtgcgcat gtggatatta tctctccagg ggcgtttgta
     9721 aaaattgggc cgattattcg cgcaacgcta aacaggtaaa ttaatattat ctataaaccc
     9781 at
//
LOCUS       DBKEKI010000149         9780 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.150, whole genome shotgun sequence.
ACCESSION   DBKEKI010000149 DBKEKI010000000
VERSION     DBKEKI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.150"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..638
                     /locus_tag="ACUQPX_003558"
     CDS             <1..638
                     /locus_tag="ACUQPX_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9058127.1"
                     /translation="LVDVFREAIAEGHVATNPVTATRTAKSEVRRSRLTANEYIEIYH
                     AAEPLPIWLRLAMDLAVITGQRVGDLCRMKWSDINDNHLHIEQGKTGAKIAIPLTLTI
                     DALNISLVDTLQKCREASGSETVIASTHHEPLSPKTVSKYFTKARNASGLSFDGDPPT
                     FHELRSLSARLYRNQIGDKFAQRLLGHKSDSMAARYRDSRGREWDKIEIDK"
     gene            complement(696..1256)
                     /locus_tag="ACUQPX_003559"
     CDS             complement(696..1256)
                     /locus_tag="ACUQPX_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HHV9058128.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKLHV"
     gene            complement(1253..2941)
                     /locus_tag="ACUQPX_003560"
     CDS             complement(1253..2941)
                     /locus_tag="ACUQPX_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HHV9058129.1"
                     /translation="MSGEKKTKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(3090..5717)
                     /gene="gyrA"
                     /locus_tag="ACUQPX_003561"
     CDS             complement(3090..5717)
                     /gene="gyrA"
                     /locus_tag="ACUQPX_003561"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HHV9058130.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALYHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            5864..6586
                     /gene="ubiG"
                     /locus_tag="ACUQPX_003562"
     CDS             5864..6586
                     /gene="ubiG"
                     /locus_tag="ACUQPX_003562"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HHV9058131.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(6714..9780)
                     /gene="yfaL"
                     /locus_tag="ACUQPX_003563"
                     /pseudo
     CDS             complement(6714..9780)
                     /gene="yfaL"
                     /locus_tag="ACUQPX_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2276 a         2732 c         2407 g         2365 t
ORIGIN      
        1 cccttgttga cgtttttcgt gaagcaatag ccgaggggca tgtggctacg aatccggtaa
       61 cagcaacccg cacagcaaag tcagaagtaa ggcgctcaag gcttacagct aatgagtata
      121 tcgagattta ccatgcagcc gaacctctcc ctatctggct gaggctggca atggatttgg
      181 ccgtcattac agggcagaga gtgggcgatt tatgcagaat gaagtggtca gacataaacg
      241 acaaccatct tcacattgag cagggtaaaa caggagccaa gattgccatt ccgctgacgc
      301 taacgattga cgcgctcaac atctcactgg ttgatacact acagaaatgc agggaggcca
      361 gcggcagtga aactgtaatc gcctcaacgc atcacgaacc gctttccccg aaaacagtat
      421 cgaagtattt tacaaaggcg agaaatgcct ccggactctc atttgatgga gacccgccaa
      481 cattccatga actgcgtagc ctgtcagcga ggctataccg gaaccagatt ggcgataaat
      541 ttgctcaacg tcttctcgga cataaatcag attcaatggc ggcgcggtat agggacagcc
      601 gtggacggga atgggacaaa attgaaatcg acaaatgatt ttattttgac caataatgac
      661 ctgctcatct taacagattg ataattaatg caattttaaa catgcaactt ccatgtcata
      721 agttgttgca gactgactgc gcgcattccg ttgtcagttt tcgtggcgct tccggtgtgg
      781 tagatgacgt aacgccccat ccagaccatt aagtgctggg catcgccctg atcgaaaaaa
      841 atcatatcgc caggcagcgc ctggtttatg tcttggccaa taaactgact gttgtactga
      901 atcaaattaa tcgcggtcac gtaggggccg tttttcccgt tcccctgatt ccagttttgc
      961 gccagttgac gctgttcagg tgttagcgtc atctctggcg gcaaatactg gctagataaa
     1021 ccgttacttt ttagccattt actgtcgtga actttcagcg tctcgttcgc cgcaaatcgc
     1081 accaggcccg cacaatcctg ctgataccag cgtggacttg gcccctggcg gagctgttct
     1141 tgtgcaatgc gtacaaacca ggcgcgaaac agcccggatt gttcgacatt cagcatttcg
     1201 ctatgggcaa caacacaaca cagccagcaa atcagcgcca gcagcccgtg tctcatagcg
     1261 gctgccaggt tatgggtagc cactgccagg cgacaccggg ttccatctgg gccagcttca
     1321 tgacataacg cggttgttga gataaagcgt ccagcttcgg cattaataaa gtttgtgcgg
     1381 cgttataaaa aaccggttcg agattcttcg gcagactggt caacgtttcg ttacgcagca
     1441 gtttcgccac gccttgtgga ttgatataga gcggaacgat gccatcagtg ggtattacat
     1501 ccaccattgc cgggcgggtt ttattcagtg tttgcagggc gttattaacc agcgtgtcat
     1561 cgagggagaa aagcagcgtt ttgttttgca tcgccagcga cacacggaaa aaataatccg
     1621 acattaattg atcgggttgc gccgcctgcg ctttcggata ctgaccgtat cgggaactca
     1681 cttcgcgaca ccagatttgc gcttcgccct gctgagtctg gcttaccggc aaaacacctt
     1741 ctggcgcttt gctttcgtgc gcaccaatat tttgcgtaaa cagtttccct ggcaattgcg
     1801 cctgttcggc agtgccatca aactgaccga caaacagcgg ggtttgcaat tttgagtctt
     1861 cataccagca cagccccgcg gcaccgtcta acgccccatt cagcttgtcg ttttcctggc
     1921 tgatgtgcga aagcatctct tcggcaatac cgtgtgaata cggcaccgcc acacagaagc
     1981 tggctccggc aggcatactg ttccagaccg gcgtaaaatc gaaactggca tctacgctgg
     2041 cggattcatc atttaacgcc acaaaactgt gccagccgtc gttacccatt tcgaagcgta
     2101 cgccagcaaa agaaggcatt aaccgctggt agccaaaacc cagcaacctg gcgctggcca
     2161 cgatgcgcag gcgtactggc gttttttcgg cagtacgctc ttccaggcca aagctggctt
     2221 gccagcgttt tttaccgctc aacaaatcac ttgcgatcgc cgtggcttcg gtatcctgct
     2281 gatcatcttt aaacaacata tccgtactgg aaaacaccag catcttgtcc tgatatgtcg
     2341 cgaacatcag ggcgttattg ccgttatagc gcaactgata aacaggaata gtttcactat
     2401 ttatcttgat gctactgatt tccgttttgc ttaactggct gtcgctggta gcggcaaaca
     2461 acaatggctc cagcagtttg cttaaaccac tgcgctggat cagcaccatg taatgtgaaa
     2521 gatggccttg tttatcgtgc catagcgccg cctgcgcggg ctgatctaag agtgacgaaa
     2581 agagcttatc tttcagcgtg agatcgtgtt catagacaat gcggcgaatg ctgccttcaa
     2641 tgcccagacg atcggcatga ttctgataat agaaaacgaa atcttcgctc agaacatcgt
     2701 ggagaaacgg aatggtgagg agatctttgg gaagctggct cagagagtcg ctgtctagaa
     2761 agaggtccgg ctcattgaga tcgatttgca gattgttgtg caccaccagc ggcgacaacg
     2821 ttttttctgg cccactgcca gcatattgca acgcccagac gccagcggaa agcagtgcta
     2881 ttgcgccaaa acctacaaga ccatagaacc gccagccttt cgtctttttt tcaccactca
     2941 ttgccacatt ccttgtgtat agccagccat tttttacggg cacagccaaa ctttaccgcg
     3001 ccctaatacg acaaaagccc agactttgca gcctggactt ttcaattcaa acaagggaga
     3061 tagctccctt ttggcatgaa gaagtaaaat tattcttctt ctggctcgtc gtcaacgtcc
     3121 acttccggag cgatttcatc atccccttcc gcggcactgc cgtcgatggt atccagatct
     3181 tcctcgtcaa ccggttcagc aacacgctgc agacccacta cgttttcatc ttccgcagta
     3241 cggatgagga tcacgccctg ggtgttacgg cccacgatgc tgatttctga aacgcgagta
     3301 cgtaccagcg taccggcatc ggtgatcatc atgatctggt cgcagtcatc tacctgcacc
     3361 gcgccaacca ctaaaccgtt acgttcggta accttgatgg agataacccc tttcgtcgca
     3421 cgcgacttgg ttgggtattc cgccactgca gtacgtttac cgtaaccgtt ttgcgttgcg
     3481 gtgaggattg cgccatcgcc acgaggcacg atcagagaga cgactttatc gccttcacct
     3541 aagcgaatac cgcgcacacc agtggtattg cagcccatcg cacggacaga agactcttta
     3601 aagcgcacca ctttaccttc ggcggagaac agcattactt catcttcgcc actggtcaga
     3661 tcaacgccga tcagctcatc gccgtcaacc agtttgatcg ccactttacc ggcggtacgc
     3721 agacggttga attcggtgag gacggttttc ttcacagtac cgttagcggt cgccatgaag
     3781 actttcacgc cttcttcaaa ctcggtcacc ggcaggatcg cggtgatacg ttcgtcctgt
     3841 tccagcggta gcaggttgac gatcggacga ccgcgcgcgc cacgagtggc ttccggcaac
     3901 tggtaaactt tcatcgaata gacgcgacca cggctggaga agcacagaat atggtcgtgc
     3961 gtgttcgcca ccagcagtcg gtcgataaag tcttcttctt taatacgtgc ggcagattta
     4021 cctttcccgc cacgacgctg cgcttcgtat tcagaaagcg gctgatattt aacgtagccc
     4081 tggtgagaga gcgtcacgac cacatcttcc tgggtgatca gatcttccag gttgatgtct
     4141 gcgctgttgg cggtgatttc agtacgacgt ttgtcaccga actgttcacg aaccagctcc
     4201 agctcttcgc ggatcacttc catcagacga tcggcgctac caagaatacg caacagttcc
     4261 gcgatctgat ccagcagctc tttgtattcg tcgagcagtt tttcgtgctc aaggccggtc
     4321 agtttctgca aacgcagatc cagaatcgcc tgagcttgct gttcggtcag gtagtacaga
     4381 ccatcacgca cgccgaactc tggctccagc cattccggac gcgcagcatc gtcgccagca
     4441 cgttccaaca tggcggcaac gttgcccagc tgccacggat tagcaaccag cgcagttttc
     4501 gcttctgcag gcgtcggcgc atgacggatc agttcgatga tcgggtcaat gttcgccagc
     4561 gccacggcta atgcttcaag gatatgagca cgatcgcgag ctttacgcag ttcgaaaata
     4621 gtacgacggg tcaccacttc acggcggtga cgaacaaacg ccgcgatgat gtctttcagg
     4681 ttcatgatct tcggctgacc atggtacaat gccaccatgt tgataccgaa agaaacctgc
     4741 aactgggtct gggagtagag gttgttgagc acaacttcac cgaccgcatc gcgtttcact
     4801 tcaatcacga tgcgcatacc gtctttgtca gactcgtcac gcagcgcgct gatgccttcc
     4861 acgcgttttt cttttaccag ttccgcaatc ttctcgatca ggcgcgcttt gtttacctga
     4921 tacggaattt cgtggacgat aatggtttca cgtccggttt tggcgtcaac ttccacttca
     4981 gcacgggcgc ggatatatac cttgccgcga ccggtacggt aagcttcttc aataccgcga
     5041 cgaccgttaa tgattgccgc cgtcgggaag tccggccccg ggatgtgttc catcagccct
     5101 tcaatgctga tgtcttcatc atcgatatac gccagacaac cgttgatgac ttccgtcagg
     5161 ttgtgcggcg ggatgttggt tgccatacct acggcgatac cggaagaacc gttcaccagc
     5221 aggttaggaa ttttggttgg catgacgtcg ggaatttttt ccgtgccgtc atagttatca
     5281 acgaaatcga ccgtctcttt ttcgagatcg gccatcagtt catgggcaat tttcgccaga
     5341 cggatttccg tataacgcat tgccgccgca gagtcgccgt cgatggaacc gaagttaccc
     5401 tgaccgtcta ccagcatgta acgcagcgag aatggctgcg ccatacggac gatcgtgtca
     5461 taaaccgccg agtcaccatg gggatggtat ttaccgatta cgtcaccaac gacacgggca
     5521 gattttttat aggctttgtt ccagtcattg cctagtacgt tcatggcgta aagtacgcga
     5581 cggtgtaccg gcttcaggcc atctcggaca tctggcagcg cacggccaac aatgaccgac
     5641 atcgcataat ccagatagga gctctttagc tcttcctcaa tgttgaccgg tgtaatttct
     5701 ctcgcaaggt cgctcatcta accgctatcc ctctactgta tcccggattc aaaggtcgca
     5761 aattataaca cagccgcgca gtttgaggta aacctatacg ctttattcac atccaatgcc
     5821 tgatatactc gtttgtcttg ccaattacgg agtagaagtg ccaatgaatg ccgaaaaatc
     5881 gccggaaaac cataacgtag accacgaaga gatcgctaaa tttgaagccg tcgcctcccg
     5941 ctggtgggat cttgaaggtg agttcaaacc gttgcaccgc attaacccgc tgcgtctggg
     6001 ctatattgcc gagcgtgctg gcggtttatt tggcaaaaag gtgctcgatg tcggttgtgg
     6061 cggcggcatt ctggccgaga gtatggcgcg cgaaggcgcg acggtgaccg gtctggatat
     6121 gggctttgag ccattgcagg tggcaaagct gcacgcgctg gaaagcggca ttcaggtgga
     6181 ttacgtgcag gaaaccgtgg aagagcacgc ggcaaaacat gccgggcagt atgatgtggt
     6241 gacctgcatg gagatgctgg agcacgtacc cgatccgcag tcagtggtca gagcctgtgc
     6301 gcaactggtg aaaccaggcg gcgatgtctt tttctcgacg cttaaccgca acggcaagtc
     6361 atggctgatg gcggtggttg gcgcggaata tattttgcgc atggtgccca aaggtacgca
     6421 tgatgtgaag aagtttatta aaccggcaga gctgcttggc tgggtggatc agaccagttt
     6481 aaaagagcga catataaccg ggctacatta caacccgatc actaattcct ttaagctcgg
     6541 ccccggcgtg gatgtgaact atatgctgca cacgcagaat aagtgaggtt gatgtttggc
     6601 cgcgccaatg cctgatgcga cgcttgcagc gtcttatcag gcctacaaat gctctccgta
     6661 ggccagataa ggcgtttacg ccgcatccgg caattgtgat ggtcagacag cgattaccat
     6721 ttcaccgtca tcgacaaaaa ccctgccgtc tgggcaaaat catcactccc tttctgccac
     6781 gccacgccac gccacgctgc cgcgcaggga cactcgctga ctgatattgc ccgtgactcc
     6841 cacttttatt tcaccccgta gcttcaccgc atcgtcactg atagtgctgc catcttcttc
     6901 aatttccgtc gcatggggat cgtgataata attcagatct aatgttggtg tgacaccaac
     6961 cgcggtacgc cattcgctgt gtaaacccag ccgcgtctga atatcatccc cctgcgattg
     7021 tgacacgcgc gcatggttag cggcggtaaa atcatcctgc tgcacgccct gataaatcac
     7081 ctgcgcctgc ggttcaatca ccacaccacg ccccggtaac cactgatacc cggcttccag
     7141 cgaggcgata atccccgacg aatggtaacg atccacgcca tcttcatgtt cggaaacatc
     7201 attgttaaac cacgcgtatt gcagccagct atccagccag gccccctgct tctgattacc
     7261 gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt tatcggcgcg
     7321 agttccggtc atattcgagc ggctgtcgcc ctgattatca ctgtagccac caaccgcccc
     7381 aagcatccac tcgccatccg tgccccagcg cccgctaaac aagccgccgc taagctgcac
     7441 cgtagaagtg tcttcctgct gagccagttg ccctgccgcc gtgtaatgat aacgtccgcc
     7501 gataacacgt aaattcagcg tctgaccatc gccaccagcg tgatcgcgtc gctccatcat
     7561 aaacgcctaa tttgccgccc gcaggttatt gagataaccg ccaactttgg cattcagcac
     7621 cggctggtaa gcaggcgtag gggccgggtc gggggttggg tcaggatccg gcgtgggatc
     7681 aggatcggga gtcgggtctg gctcaggtgg cgatggcggc gttacttctt gcgatcgcag
     7741 ataccagtcg ttgttatctt ccactagcgt gtagtcatac gctcccatgt tgacgtagcc
     7801 actgccagcc agactgaact gtgcattgtt ttgaaactgc gtgggatcag ctgcgaaatc
     7861 aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatgaagt taaccaccac
     7921 agttgtgttg ccagcagtat taccgttcat caccaattga tcgcttaccg agtcatcgcc
     7981 gtttaattcg ctgtcgaata gtagcgtacc gccgccggta taatcaccgt ttaccgtcag
     8041 cgtatcgcca gcgacaccat tttgcaggct aaccgcaccg gacgtattgg tcagatcacc
     8101 attaaccgtc acgtcagcaa gataggtcag gctgtcagtt tcaaggttgg tattaagctc
     8161 gcctgtcaac gtcacgccat cggtagcatt taccagcgtc ccatcgccgt taaatagtga
     8221 ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac catcgctgat
     8281 gtcgatagtg ccgctggaag tagcatcaat tgactgaata tcctgatccg cgccagtaac
     8341 gaacgttgcc ccatcaccaa cccacagtga cgaagcataa ggaaagatat ccgcaacatc
     8401 gcctttcagg gtgccttctt cgacacgtac cgccgactgc gttgtaccgc tggcggtcag
     8461 ctccagcgta cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat gctgcccgct
     8521 gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg ccacttcacc
     8581 gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag tatcgacatt
     8641 ggcagtaatc tccatcacgc tttcttgttg cttatcggta aagataacct ggcggttata
     8701 gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg cgatttgtgt
     8761 tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg catcgaccac
     8821 cacgtagacg ccatctttcc cgtcgccgcc gatatgcaaa ttgtcgccac ctgcaagatc
     8881 taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa ggccatcatt
     8941 gtatgatgtg ccgctctgcc aggtggagaa atcagagaga tcgagcacac cgccgtttaa
     9001 cacgatcgac tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc cttccagcga
     9061 aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact gcgcccctgc
     9121 cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga tgatgccagc
     9181 aaaactgccc tgattaacag taacgttgcc accagcatcg atatttaaag tgccattctg
     9241 aaagcccgtc aatgcgtgca caaaagtttg ttgggtcgag ccaacgttta gctcagcctg
     9301 attctgatac tgatcaatac tccctatcgt cagaccgtag cagtcttgcg gatcgtcctg
     9361 gcaatgcgta tcgccgacat tcatcaggga gttgctgcga cccagggtaa cttcaccgtt
     9421 ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca gatcgccgga
     9481 acctgttttg gtgattaacc cggtaccagc aatagagtca acagctccgt cattctctgt
     9541 attgccgata accagcgttt ttccgtcggc aatatcaaag gtaacttcac ttaagccgag
     9601 atacataaag ccacccgccg cagtggaagg accatctcca tagcctgctg cgctattgtt
     9661 ctcatcgact aacacgccgc cgttctggct gtagctgtca tcaacagaaa tatcaataag
     9721 atagggagcc gtcgcgctat tggtatatat cgccccgcca taaccttcgg ctgtgttatt
//
LOCUS       DBKEKI010000150         9721 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.151, whole genome shotgun sequence.
ACCESSION   DBKEKI010000150 DBKEKI010000000
VERSION     DBKEKI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.151"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..480
                     /locus_tag="ACUQPX_003564"
                     /pseudo
     CDS             1..480
                     /locus_tag="ACUQPX_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2085..2303
                     /locus_tag="ACUQPX_003565"
     CDS             2085..2303
                     /locus_tag="ACUQPX_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HHV9058132.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2521..2763
                     /gene="yiiF"
                     /locus_tag="ACUQPX_003566"
     CDS             2521..2763
                     /gene="yiiF"
                     /locus_tag="ACUQPX_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HHV9058133.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3093..4022)
                     /gene="fdhE"
                     /locus_tag="ACUQPX_003567"
     CDS             complement(3093..4022)
                     /gene="fdhE"
                     /locus_tag="ACUQPX_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HHV9058134.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4019..4654)
                     /gene="fdoI"
                     /locus_tag="ACUQPX_003568"
     CDS             complement(4019..4654)
                     /gene="fdoI"
                     /locus_tag="ACUQPX_003568"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HHV9058135.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTNAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4651..5553)
                     /gene="fdoH"
                     /locus_tag="ACUQPX_003569"
     CDS             complement(4651..5553)
                     /gene="fdoH"
                     /locus_tag="ACUQPX_003569"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HHV9058136.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5566..8616)
                     /gene="fdnG"
                     /locus_tag="ACUQPX_003570"
     CDS             complement(5566..8616)
                     /gene="fdnG"
                     /locus_tag="ACUQPX_003570"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8029..8031),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HHV9058137.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTETYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8810..9643
                     /gene="fdhD"
                     /locus_tag="ACUQPX_003571"
     CDS             8810..9643
                     /gene="fdhD"
                     /locus_tag="ACUQPX_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HHV9058138.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2416 a         2356 c         2492 g         2457 t
ORIGIN      
        1 taaataagga tggcagtctc cagagtaagc actacacact tgatgtaggt gaagttataa
       61 acagttataa tattgaccct ctttgtgaat gcttcaagta ttaatcaaac ttacttacat
      121 gataaaaatc ctggtgacgt tattagcctg tatggcactt tagataacat tccccttgta
      181 gttaaggaat gcacgggtaa aaatcatcaa attaatgtta aagccaaatt gccacaagaa
      241 cgaagtgaaa aagttgagga tatgtgtgaa agaatgaaag gtggcatctc tatgtttaac
      301 cacaccacaa aaacagctga taatatagag cataatttgc agctaagttt tctcgtgggt
      361 tcacgtccgt cgatcaccaa taaatactca gcaaaagatg taggtccaga tatacttgca
      421 ttgcccgtca acgtatatca tgcggatttt aaaattaaaa tcaataatgc agtggagaag
      481 gcttcatcaa ttgtgttatg tgctgtgcaa aaaaatctcc atgcagcaag gaaggttctt
      541 agcgggataa taggaaatgt gccaaaaaat aataaaccaa aaacaattat ttttgatttg
      601 cgttttctta gtgcaattaa gcagaaagtt tttttgggca gtgaaaaacc gttgtttgta
      661 aagcataccg caatgatgag ggaggcatgt aaggaattac cgcagtcagt ggaatatgtt
      721 cctttagcct gtgaagcgca tagccatcgc agcgtcgccc ttgccaaaac cgtggctgcg
      781 cccatcatgg cgacaggtgt gggcttggca ttaatgtatg caacatcatc aggaccgtat
      841 tatgcgcaaa gtctttctgg ttcgccggat atcgagatga taaaaaatcg catggctgaa
      901 ttgtttaacc agtcggatat ttctgtgcgc aacaaatata gcccggcttt tgataaatgg
      961 aatgaactgg tagataaatt aaatcacgaa agaaatacag ttccgtttgc gtgtttgacc
     1021 accattttag cgacggcgat aaatgaaact cttgaggggg atacgaatgt tgccgttgtg
     1081 atggggtgta aatctgcgaa agatcgcacg atatccattg tccttggcaa ctcgatgttg
     1141 caaacactcc ttgaaaaacg cctcaccgat agtggagaaa tagaaaatct ttttgatcaa
     1201 cagggatatt ttagttgcga ttcattaaca cccgaagaat taatgatgtt aaaagacctt
     1261 tttgatattc gagttcttca tgtctctaac aaattcaatg ttggattgca aggtaatatc
     1321 aacactgatg ttttacaaga ttcgtttttt aaaaatgtcg atttcattca tgaatataac
     1381 tcgtataccg tagggatggg agtctgacga gtaaaactca ttaggattat cagaattcgg
     1441 acaatgcatt gcaaaaatga catacaaaga gagatgtgtg caggtactta gccgcgaggg
     1501 gcatggcgaa gccatacaga aagtaccagt attcagaact ccagtgaaag cggcatagtg
     1561 gccgcctgct caggagtttt gctatgaccc gtaatcgtgc tgaacgtcgc caccctatga
     1621 tgcgattaaa aaaactcatc gtcgcgctga tgattgacgt caatgctatc ggatgatgcc
     1681 gggggtgttc cggaaggaat tccttgtttc ccgaactgtt accaacaaaa ttataacgat
     1741 gagcaagtaa agaccggttg gtggcagtac tgaccggtct gtgatgaaag attaatttca
     1801 ccttaaatcc tggtgctgtt aggatgctga cgtttttcgg ctacaccatt cagattatgc
     1861 aaataaatat ccctgaatat aatatattca tagcacccat aactcatcct gggcgataac
     1921 taaaccattt ttgagtaata aatgaaacaa ctgtaacgct cttattattt ctggaaaacg
     1981 cctcgcaaaa acaaaatgac atgcaaaaat ccgctttttt taaatgcttt tccggcgtaa
     2041 ttttcgcaca tacattaatg agtaatatat gtaaggtggt ggtaatggcg atgaatacgg
     2101 tttttcttca tttatcagag gaggcaatta aacggctgaa caagcttcgg ggctggcgta
     2161 aggtttcacg atctgcaatt ttacgcgaag cggttgagca atatctggag cggcagcaat
     2221 ttccggtgcg taaagcaaag ggaggcaggc aaaaggggga ggtagttggt gttgatgatc
     2281 agtgtaagga gcataaggaa tgaaaaaaat ttttcattct tgaatataaa aaacagatgc
     2341 ccttattctg gtattaatac aaggctgttt tacttaaact tataataact gcaactgtta
     2401 catcgtatct ggaaaacgcc tcgcaaaaat aaaaaatgat gcgtaaatga gctttcttta
     2461 aagattcggc aagggtactt ttctctccat agattcaaaa atgatattgg cgagatattt
     2521 atgaactctc tggcaggtat agatatgggc agaattttac tcgatttatc gaatgaggtg
     2581 attaagcaac ttgatgatct cgaggtgcag cgtaatcttc ctcgtgcgga cctattaagg
     2641 gaagcggtag atcaatacct gataaatcaa tcgcaaacag caagaaccag tgttcctggc
     2701 atctggcaag ggtgtgagga agatggtgtc gaatatcagc gtaagctgcg cgaggaatgg
     2761 taatgggaaa aaatgatgtc aatcagattg ctgacaacgt gcgcgttgtt catgccggat
     2821 gcggcgtgaa cgccttatcc ggcctacaaa gtcatgcaaa ttcaatatat tgaagtttat
     2881 tggtaggcct gataagcgca gcgcatcagg caatcatacg cttgtcatca gtgacatacc
     2941 cataacctta tcgatgtgtt ggagggaaga acgaggcaaa cagacaattg cggcatgatc
     3001 ttgaatgggt aacgcggaat acgagagatg gtgtcgtcac accctatgag ctgtaatccg
     3061 ggcgacctgc gccacccgga aaacgtcaga gattactctc cttcacccgg aaacaggaac
     3121 gggttaatgg aactacgggc gtagccttct tgctccattc gcgcgtccag taccagagag
     3181 gcgaggtcat ctgccacggc ttcaactttc ggttcttttt cctgataaag aatcttcagg
     3241 taagtgccgc agtcatcgca gctttcggct ttaatcgcgg cctgttcgtc atccagcgac
     3301 cagtaatgca gtttgccgct ctgctcgcag ttgctgcatt ttacgcgcac tacgtgccat
     3361 tcggtttcac acaggttgca gtgcaggtaa cgcagaccct gagtggtgcc aatttgcacc
     3421 atgctggaca ccggcataga gccacatacc gggcaatatt gacgttgttc gccgtattca
     3481 gcgcgggctt tgccggggat cagattggcc atctgcgccc agtagagcga cagcgcagcc
     3541 cagataaacg gcgctttatc gctgctgacg gacgagaaat cagaggcaaa cagcgcgctg
     3601 gccatatctt ccagctcctg agtcgatgcc ttctccagat tctcaatcac tgccagcgcc
     3661 gggccgctca tttcaggttt cagctcagca attagcgcca tcagcagctt ttgccagtgc
     3721 ttatcacgcg gcagaacgtg aatatccagc gggggcttgc cctgtgcgct ggcttcttta
     3781 atgcgcgcgg tcagatccat ctccagcgga tggtcgtaca gcaccacttc ctgggcgtgg
     3841 gcgataagcg cagcaaagcg caggtaatca cccagcggat tattttctgc cagctcgcgc
     3901 agacgctcgg cgcggcggtt gtataaattc ttgagccgag ggaacaataa cggcggaatc
     3961 atatccgccg tacgtttctc gctcgaaccc agctcatctt gcgggattat gcgaatactc
     4021 attcagcttt cttttccgtt gtcttgcgga cctcacggta ccagcgcggg tgatgtttct
     4081 tcgcccaggc gttggtaacc catccttcca ccatggcggt aatcgtgcct ttcacccaaa
     4141 gggcggcgta gatatgcacc atgataacca caattaacgc cactgcggca aatgaatgca
     4201 gcattaacgc gaatcggatc accgggattg agaaagcagg cgcaaaataa ggacgccaga
     4261 taatcacgcc gctcaccagc agcagaacca ggaaaataat cgccgcccag aaaacgcatt
     4321 tctgaccgaa gttataacgc ccggtgtcac ctacttcctc gttgacgacg atcttacgaa
     4381 tattcttcgc ccaaaagata tcatcccgat tgattaggtt ttgatgccag taacggaaaa
     4441 acatgatgat gaacgaggca aacataacca cgccgacaaa cgggtgcaga attcgcgcca
     4501 gctgcggtgt gcccatgatt tgcatcaacc agttgaagga cgggaacaaa aagcccagcc
     4561 cgctcaccgc cgccaggatg aagcagaagg cggtgatcca gtggttgata cgttccggcg
     4621 cggtgtagcg cacgatggtg tcacgtcgtt tcatttgcgc tcctcgtctt tctcttcgtg
     4681 cagattattc tcttcctcat ccgcacggtt cggaccgaca cccacgtagt ggaagatact
     4741 ggccgcaaag gtagccgcaa agccaacagc cgcgagcggt ttccagatgc ctttccagaa
     4801 tttcacggtt tcgctgattt ccgggttctc cggcaagcca tgatacagat ttggcttgtc
     4861 agcatggtgc agcacgtaca tgacgtgtgt accaccgacg cctgccggat cgtacagacc
     4921 cgcattgtcg taaccgcggg ttttcagctc agcaacgcgc tcgctcgcca gcgttttcat
     4981 cgactctttc gtaccgaagt gaatcgcgcc cgttgggcag gtcttcacgc aggctggttc
     5041 ttgcccaacc accacgcggt caacgcacag cgtacatttg tagacgcggt tgtcttccgg
     5101 gttgaggcgc ggaatgtcga acggacagcc cgcaatgcaa taaccgcagc caatgcactg
     5161 ctcggactgg aagtcgacga taccgttggc atactgaata attgcacctt ccgccgggca
     5221 cgctttcagg cagcctggat cggaacagtg catacagccg tctttgcgga tcagccattc
     5281 cagtttgtcg ttctgctcca cttccgagaa gcgcattacc gtccacgatt tggcgcttaa
     5341 atcattgggg ttgtcgtaca ccccaatgtt attgccgacg gtatcgcgaa tgtcgttcca
     5401 ctctgaacac gccacctgac aggctttaca gccgatacag gtggtaacgt cgatgagttt
     5461 cgccacttct tcctggaagt cccgcgcctg aggcgcgggg gtcagaccgt tagtcgcgga
     5521 acgacgaatg atatcttgag attgataagc catatgtcgt ctccgttaca ccttttccac
     5581 attcacaagg aaggacttaa actccggcgt ctgcgtgttc gcatcaccaa cgaatggcgt
     5641 taaagtattg gcaataaagc cttttttagc tacgccttca tagccccagt gaatcggaat
     5701 accgatggta tcgatatctt tgccgttcgc tttcagcgtg cgaatacgtt tggtcaccac
     5761 cgctttggct ttgatatagc cacggttgga ggagactttc acggtatcgc cctgggcaat
     5821 gccaagttta ttcgccagcg actccccgat ttccacaaac tgctctggct gcaaaatcgc
     5881 gttcaacagc gcgtgtttgg tccagtagtg gaagtgctcg gtcagacgat aggtggttcc
     5941 gacatacggg aacttatcgg ctttacccaa tgcttcggcg tcgtctttaa agatacgtgc
     6001 agccgggttc gagataacgt ttggatgcag cgggttagtt cccagcggcg tttcaaacgg
     6061 ctcgtagtgt tccgggaacg gaccttccgc catcttatcg agggcaaaca gacgccccat
     6121 gccttcctgc tgcatgataa acggcccgac gccgctaccc ggaggcgctg cgctgtagtc
     6181 cggaatatcc cagccggtcc acttagtgcc gtcccatttc agcaactgac gcttcggatc
     6241 ccacgggtta ccctgcggat ctgcggaggc gcggttatac agaatgcggc ggttaagcgg
     6301 ccatgcccat gcccagccca gcgtgttacc gaggccagac ggatcggcgt tatcacgacg
     6361 cgccatctgg ttgccttccg gcgtccagct accggcgaaa atccagcagc cacaggaggt
     6421 tgtaccgtca tcgcgcaatt gggcgaacga gctaagttgt tggcctttct tgacgataac
     6481 cgcaccggtt gccggatcgg taatatcggc cagcgcctta ccgttgctct ccatcgctac
     6541 ttcttccgac ttcggctcat gcggaatggc gtagttccag gtcatgttca gcacctgatc
     6601 cgggttcgcg ccaccctgtt cggcatacat cttgcgcaag cgcaggaaga taccggagag
     6661 gatctcaccg tcagtcagcg caatccccgg ggcgtccgca cctttccagt gccactgcaa
     6721 ccagcgacct gagttaacga ttgaaccgtt ctcttctgcg aagcaggtcg atggcagacg
     6781 gaacacttcg gtctggatct tcgacgagtc aacttcgttc agctcaccgt ggttctgcca
     6841 gaagttagag gtttcagtgt tcagtgggtc gatggtgacg aggaacttca gtttcgacag
     6901 acaaccgatc actttgtttt tgttcgggaa tgaggcaaca gggttaaagc cctggcagat
     6961 atagccattg accttgccct ctttcatcat ctcgaagtat tgcaggacgt cgtagccttt
     7021 atcccacttc ggcaaccagt caaagcccca gctattttcc gccgtcgctt tatcaccaaa
     7081 gaaggccttc atcatagaga cgaagaattt cgggtagttg ccccagtagt ttacctggcc
     7141 ttccagcagc ggttttggcg tgttggcggt aaggtaggtt tgcagatcgg tctgcttctc
     7201 gcttggcagc gtcatgtaac ctggcagact ctgcgacagt agccccaggt ccgtcagccc
     7261 ctgaatattg gagtgaccgc gcagggcgtt aacgccgccg ccggccatcc ccatattgcc
     7321 gagcagcagc tggatcatcg ccatcgtacg aatgttttgc gcaccaacgg agtgttgcgt
     7381 ccagccgagg gcatacagga acgaggcagt tttatcgtga gcactggttt ctgcgatgta
     7441 ttcgcagact ttcaggaacg cgtcttttgg cgtaccacag atgttttcaa ccacatctgg
     7501 cgtgtagcgg gaaacgtgct gtttcagcaa gttccacacg cagcgcggat gttgcagcgt
     7561 ggtatcgcgt ttggcgaagc cgttttcgtc cagttcataa gtccaggagg atttatcgta
     7621 cttgcgtttt tccgcgtcgt agccggtgaa caggccatct tcaaagccgt aatcctcacg
     7681 cacgatcagg ctggcgttgg tataggtttc ggtgtattcg tggttgaatt tttcattgtt
     7741 cagcaggtac agcaatacgc ctgacaggaa agcaatgtca gtaccggaac gaataggggc
     7801 atagtagtca gctaccgccg ccgtacgcgt aaagcgagga tcgatcacaa tcagcttcgc
     7861 gccgttgtga attttggctt ccatcgccca gcggaacccg accgggtgag cttcagcggc
     7921 gttaccgccc atcaccacga cgaggttggc gttcttgatg tcgacccagt ggttggtcat
     7981 cgcaccgcga ccaaatgttg gagcaagact tgctaccgtt ggtccgtgtc agacacgcgc
     8041 ctggttgtcg accgcgagca tacccagcgc gcgggagaat ttttgcgtta aatagccggt
     8101 ttcgttactc gacgcggaag cacacagcat cccggtggag agccagcggt taacagtcac
     8161 gccttcggcg ttttgcgcaa tgtagttagc atcgcggtct tctttcatca gtttggcgat
     8221 gcgatcaaac gcctcttccc aactgatttg ctgccattta tcagaacctg gcgcacggta
     8281 ttccggaaac ttcagacggc tttcggagtg gatgaaatcc accaggccag cgcctttcgg
     8341 acaaagtgca ccgcggttga ccgggtgatc cggatcgcct tcgatatgga agatagatgc
     8401 tttggcgttt tttgctccgt caccgaggct gtacatcaac agcccacagc ctacggaaca
     8461 ataggtgcag gtattacggg tttcgcgggt gcgcagcagt ttatactgcc gggtttccgc
     8521 gagtgctacg ctgggtgcaa aacccagtgc cgctgccgtg gtgcctgcca taccgccagc
     8581 gcagatctta aagaactgcc ttctgctgac ctgcatggat tgctccttgt ttcgacattg
     8641 tcacgtccca tttacattcg cttgctgcgt gtgcagggag tgggagttat ttttctttgc
     8701 ggaaggggcc gcaaaggtcc agaattggct caatttccct ccatctagga agggtttgta
     8761 acagaatacc ataatgttgg tgtgtgtgtt cttatctggt taagagaaag tgaaaaaaac
     8821 acagcgaaaa gaaatcgaaa atgtgacaaa tatcacaggt gttcgtcaaa ttgagttatg
     8881 gcggcgtgat gatttacaac acccacggtt agatgaggtc gcggaagaag ttcccgttgc
     8941 gctggtctac aacggtattt cgcatgtggt gatgatggcg tcgcccaaag accttgagta
     9001 ctttgcgctc ggtttttcgc tttccgaggg gataatagaa agtccgcgcg atatcttcgg
     9061 catggatgtc gttccttcct gtaatggtct tgaagtacaa attgagcttt ccagccgccg
     9121 ctttatgggg ttgaaggagc gccgccgggc gctggcggga cgtacgggct gtggcgtatg
     9181 cggcgtggag caacttaatg acatcggaaa accggtgcag ccgctaccgt tcacccagac
     9241 gtttgatctc aacaaactgg atgatgcatt acgtcatctc aacgattttc agccagtggg
     9301 gcaactgact ggttgtactc acgccgctgc ctggatgttg ccatctggcg aactggtcgg
     9361 cgggcatgaa gacgtgggtc gccatgtggc gctggacaaa ctgttaggcc gccggtcaca
     9421 agaaggggaa agctggcagc aaggtgcggt actggtttcc agtcgtgcca gttatgaaat
     9481 ggtgcaaaag tcggcgatgt gcggcgtaga gattttgttt gcggtgtctg ccgcgaccac
     9541 gcttgctgta gaagtggctg agcgctgtaa tctgacactg gtaggttttt gtaaaccggg
     9601 tcgggcaacg gtttataccc atccgcagcg tttgagcaat taattaaaat aagtcctgcg
     9661 atatatatta aattgcagga cttattcatt tcgtgaattt tattatttta tttataaata
     9721 a
//
LOCUS       DBKEKI010000151         9658 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.152, whole genome shotgun sequence.
ACCESSION   DBKEKI010000151 DBKEKI010000000
VERSION     DBKEKI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.152"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(281..1039)
                     /locus_tag="ACUQPX_003572"
     CDS             complement(281..1039)
                     /locus_tag="ACUQPX_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HHV9058139.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1047..2150)
                     /locus_tag="ACUQPX_003573"
     CDS             complement(1047..2150)
                     /locus_tag="ACUQPX_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHV9058140.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2160..3341)
                     /locus_tag="ACUQPX_003574"
     CDS             complement(2160..3341)
                     /locus_tag="ACUQPX_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHV9058141.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3409..4434)
                     /locus_tag="ACUQPX_003575"
     CDS             complement(3409..4434)
                     /locus_tag="ACUQPX_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HHV9058142.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4865..5086)
                     /locus_tag="ACUQPX_003576"
     CDS             complement(4865..5086)
                     /locus_tag="ACUQPX_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058143.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5339..8435)
                     /gene="acrF"
                     /locus_tag="ACUQPX_003577"
                     /pseudo
     CDS             complement(5339..8435)
                     /gene="acrF"
                     /locus_tag="ACUQPX_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(8447..9604)
                     /gene="acrE"
                     /locus_tag="ACUQPX_003578"
     CDS             complement(8447..9604)
                     /gene="acrE"
                     /locus_tag="ACUQPX_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HHV9058144.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLYPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2561 a         2511 c         2318 g         2268 t
ORIGIN      
        1 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccagac atcgaataaa
       61 acaaaaggcc cagtcttccg actgagcctt ttgttttatc tgttgtttgt cggtgaacac
      121 tctcccgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
      181 agggtggcgg gcaggacgcc cgccataaac tgccagacat caaatcaagc gaaaggccat
      241 ccgaaaggat ggcctttttg cttttcgaac taacattcaa ttaatggatt acctgcgata
      301 aaaatgccct cgtacgctct gatttaggat gcgcaaaaaa ttcatcaggt gtagcttgtt
      361 ccactatctc cccacgatcc ataaaaatta cccggtcagc gacggtacgt gcaaacccca
      421 tctcatgtgt tacacacaac attgtcatac ccgactgcgc cagcccaatc attgtatcca
      481 gcacctcttt caccatctcc ggatcgagcg ccgacgttgg ttcatcaaac aacataattt
      541 tcggcttcat acacagcgaa cgcgcaatgg caacgcgttg ctgctgacca cctgaaatct
      601 gtccgggaaa cttatgcgca tgttcggcaa ttctcacccg ctcaaggtaa tgcatcgcca
      661 gagcttcagc ctctttctta ggcatcttgc gtacccaaat cggtgccagg gtacagttct
      721 gtaaaacggt cagatgaggg aagagattga aatgctgaaa taccattccc acttcctgcc
      781 tgacgcgctc aatattgcgg atatcttcat taagttcgat gccatctacg acaatccgtc
      841 cctgttgatg ttcttccaga tgattaatac aacgaatggt tgtcgattta ccggaaccag
      901 aagggccaca cagaacgatc cgttctcccg gttgcacggt taaatttata tttttcaaaa
      961 catggaattg tccataccat ttatggacgt tttccagcgt aatcatcgcg ttagcaggtt
     1021 gcagtaaaat ttggctcata gtgtcctcaa tgcggtgtac gcccggtgtt aaaacgtttt
     1081 tccagatgtt ggctataacg tgacatgctg aaacaaaaga tccagtagat cagcgcggcg
     1141 aaaacatacc cttccgtcga catacccaac caggcgggat caacggttgc ctgctgaacg
     1201 ctactgaaaa gatcgaacaa gccgatgatg atcaccaggc tggtatcttt gaagagtgcg
     1261 atgatggtat ttaccagccc aggaattacc agcttcaacg cctgtggcag aataaccagc
     1321 ccctgagttt tccagtaacc caacgccagc gactctgccg cttcatattg ccctttaggc
     1381 agcgcctgta atccacctcg cacgacttcc gcaacatatg ctgactgaaa caggatcacg
     1441 ccaaccagcg cgcggatcag tttgtcgata ctggtgcctt ctgccataaa caacggcagc
     1501 atgaccgaag acataaacaa tacggtaatc agcggtacgc cgcgccagaa ctcgataaaa
     1561 atgaccgata agatacgcac aatcggcata tgggagcggc gacctaatgc cagtaagatc
     1621 ccccacggta gcgcccccgc aatcccaacg gatgcaataa ttaaagttag cgtcagcccg
     1681 ccccattgcc gggtttcaac ccgctcaaga ccaaaaaaac cgccatacat cagccaccag
     1741 acaatcagtg ggtaaatcac cgcccaggcc gcaatatagc gaccgcgatg cgggagtatt
     1801 ttccagaaca ttggtgcgat cgatacaagc ccaatcagta atgcgaggtt aattcgccag
     1861 cgttggtcgt gtgggtaaag cccatacata aactgaccaa atcgttcgtg aatgaagacc
     1921 caacaggcac cggcttttgt gcagtcagca cgcgttgagc caacccaatt agcctgcaaa
     1981 aatgcccagt tcagcaacgg tgggatcagt tcccacatca accatatgca gccaatagtc
     2041 agcaggctat tgctccagct ggagaacaga ttttttcgca cccacaccat cgcgcggctt
     2101 gagttatggc tcgccgggcg cgagggctga gacagcaata cttttgtcat catgactcct
     2161 tagcgttcaa cgatcgcgat gcggcggtta tagatattca tcagcagcga gatagtcagg
     2221 ctgataatca gatagaccga catggtcatg gctatcgtct cgatggcttg ccccgtctgg
     2281 ttcagcacgg tgccagcaaa cagcgaaacc atatcgggat agccaatagc ggcggcaaga
     2341 gaggagtttt tgacgatgtt gagatactgg ctggttaacg gcggaataat tactcgcagt
     2401 gcctggggaa taatgacctg gcgtagcgta accgggttgg gtaaccccag tgaccgagcc
     2461 gcttcatgtt gaccataagg cactgcctgg atcccagcgc ggataatctc ggcgataaat
     2521 gcagaggtat aaaccgaaag tgccagcgtt aaggctgcca gttcaggaat taaaaccatt
     2581 ccgccgcgga agttaaagcc ttgtagggct gggacatccc agtgtagtgc tgcgccaaaa
     2641 agccattgcg ccagtaaagg caaaccaatg atcaacactg cggcgatcgg ccaggagcgg
     2701 cgcagttgtc cggtctttat ctggtatgtt ttattaaagc ggaatagccc aacagaaagg
     2761 actatagcca taacaacagc caggataaac gcaataaatc cgtctcccag ctgcggtgac
     2821 ggaatataaa gcccacgatt gctcaaaaag gccagatcaa acgcgctcac agcttggcgt
     2881 ggtccgggca aattgcgcaa cacggcaaag taccagaaga agatttgcag cagcggggga
     2941 atattacgga agatctcaat ataaattgtg gaaagctttc gtagcagcca gttatccgaa
     3001 agtctcgcca gaccgataaa gaagcccaaa acagaagcga acacaataca caatgcagaa
     3061 accagtagcg tattgagtaa gccgacaata aaaacgcgtc cgtaggtgtc gccctgctgg
     3121 taatcgatca aatgctggac aataccgaag ccagcgccgc gatccagaaa ggcaaaacct
     3181 gaagtaatgc cacgattatt gagattcgtt acagtgttgt gaaataacca accaacaatg
     3241 ccgacaacag caacaacggc aaggatctgg aataaccagg cacgaaccgt agggttggca
     3301 aaggagagtg agcctttaac ggttgagcgg cgatgagaca tagaaacctc ggaaagcata
     3361 acgctgaaga gagcgccgcc gtagcgacgc tcgttacctc acaccgactt aacgcacggg
     3421 cggcgcgtac tgaataccgc cgttattcca gagattattt tgcccacgtt taattttcag
     3481 cgggctttct gaacctacgt tacgctcaaa aatttccgag tagttaccca cctgtttgat
     3541 gatgttatag gcccatttat tatccaactt cagatccttg ccgtaatcgc cttctttacc
     3601 caacagatgt gccatatcag gcgttgctgg attagccgct ttttcatcga cgttctggga
     3661 attgatgccc atctcttcag catttagcat ggcgaaaagc gtccagcgta caatcgagaa
     3721 ccattcatca tcgccacgac gaactaccgg cccaagcggt tctttagaga taacctccgg
     3781 taagacaatc cattcagccg ggttgcttaa tttgatgcgc aaggcataca gttgcgactg
     3841 atccgaagcc agcgtatcgc agcgaccaga ttccagtgcc ttcgctgatt cgtcagagcg
     3901 atcgaaagtc actggtgtgt acttcatatt gtttgccttg aaatagtcgg cgacgttgag
     3961 ttcggtatca gtacccgcct gaatacagac ggtagcgcca tccagttctt tcgcgctttt
     4021 tagccccgct ttatcgtgcg tcaggaagcc aatgccgtcg taataagtga cgccggtaaa
     4081 tgccattccc atcccggcat cgcgagatga agtccaggtc gtattacggg agagcaaatc
     4141 cacttcccct gactgtaaag cggtgaagcg ttcttttgct gtgagtgggg tatatttcac
     4201 tttcgtgtcg tcaccaaata cagcagcggc aacaccacga caaacatcaa catcaatacc
     4261 tgaaaactta ccgtctgcat cggcataaga gaacctaggt aatccatcac tgatcccgca
     4321 ttgcacaaaa ccttttttct gaacggcatc aagcgtcgcg ccagcatgcg cctgatttgc
     4381 aacggcaagc agcacgctgg cggcagccag tgtggctatc atcatctttt tcataatgct
     4441 tcctgtgagg cgaaaattat cgttatagga cgtttgggaa acgtctttta cctgtctgtg
     4501 gccatcctct tataagcaaa agtagtgcca gcttttgcgc acgatttcgc gtcgcaataa
     4561 gctgagtgta gacaggaaat aataaaaaca gcgccccgca atggtgcgaa aatgaaacat
     4621 gcgccacatt ttggcgcaaa atatttacgc gtgttttact aagcagaata atgattgtta
     4681 gcgtgagtat atttctggtg ggaagtgtga aagttaaatt gatgcgttat ttcgaaagca
     4741 gcgaaataac gcgatataac atgctaaatc agcagagaat tagccagcag aatatagcaa
     4801 tattttatct tatgtaaaga tactgtatcg ttaaacagag cgcgaaactc cgcgccctgt
     4861 tcagttattt cgacagagcg atgatcccca tcgtcaggcc agcaacagcc gctgccccgc
     4921 cagcaatagc cccagccgtt tcccagttat cctgagtcgt gcctttcata caggtattgg
     4981 tgtgaaccag acgaccctga tcgtcataaa ccggaacgca gggcgaatcg tgagcacagc
     5041 ccgccagcaa tgcggtgagc aatgccacag gaattaatct tttcatggtg ttacctcagt
     5101 aataaacgct aacgcttaat aagttaactc tcttattaac gagctgaatg ccattttatc
     5161 accggaggtt ataaatcttg cggaatgaat aatgacaaat tatgtgaatt gtaaaaatag
     5221 tgaagggagc gggaactata gaaagctttt aaagagtgag cagaagaata tcttattaat
     5281 ttttatgggt ttcactggaa ataataaagg cacccgaaag cgcctttatg tttctgattt
     5341 atcctttaaa gcaacggcga atcaccacaa agaacaccgg tacgaagaag attgccagca
     5401 acgttgcaga gaccattcct cccattaccc cgatacccac agcgttctgc gcgccactgc
     5461 cggcaccgtt actgatagct agcggtaata cgccgagaat aaaggcgaga gaggtcatca
     5521 ggataggacg cagacgcata cgtactgcca tcagtgtcgc ttcaacaaca cctttaccct
     5581 ctttctccat gagatcttta gcgaactcaa caatcaaaat agcgtttttg gccgacaagc
     5641 caattgtcgt tagcaagccc accataaagt agacgtcatt tttttgatta aagagtgtcg
     5701 ccgccagcag cacgccgaca atccctaacg gcacgaccaa cataaccgag acaggaattg
     5761 accagctttc atagagtgca gcaaggcaca ggaaaacaac cacaaaggaa attgctacca
     5821 gagcgggagc ctggtttccc gataagcgtt cctgatacga catacccgtc cagtcataac
     5881 caatgcccgc aggtaatttt gacgcaaggt tttccatcaa cgccatggca tcgccggaac
     5941 tggttcctgg cgcggcttcc ccctgaatct ccattgacgg cagaccgttg tagcgttcca
     6001 gtcgcggaga gccatacacc caatgtgaag tggtaaaggc agagaatggc accatttcgc
     6061 cgttggcgct gcggacataa agtttatcga catcttctgg cagcatacgg aatttggcat
     6121 ccgcctgaac atacaccttt ttcacgcgac cacggtcgat gaagtcgtta acgtaagtcc
     6181 cacccaacgc cgttgaaatg gtctgattga tgtcagaaag tgaaacacct aatgccgcgc
     6241 cttttcctgg tcaacttcca gtttaaactg cgcggtgtct tccaggccat taggacgcac
     6301 gctgactaag ctggcaggat gttgcgccgc cataccaagc aactggttac gggcctgggt
     6361 tagggcatcg tgacccagcc cagcctgatc aattaactca aagtcgaaac ccgttgccgt
     6421 gcccagttca acaatggctg gcatattgaa tggaatgaca aaaccgtcgc ggatcttgcc
     6481 caattccatt ttggcacgat ggattaccgc ttccgcactg ttttcatcac cattacgctc
     6541 ttcccacggt ttcagactga cgaaggccat accggcgttt tgtgcctggc cgctgaagct
     6601 aaagccgtta accgtaaaga cactttcaac gttcgctttc tcgttcttca gatagtaatc
     6661 cgtcacttga tccaacactt tttgcgtccg ctcttgcgtc gcgccagcgg gtaactgaat
     6721 catggtcaga aagacaccct gatcctcttc aggtaagaag gaagacgtaa aaacaacacc
     6781 accattcctg caacaatcag cgcatagatc agtaaatatc gtcctgtgga accgaggatt
     6841 ttgccgacgc tgttggtgta gtggttaacg ctatgatcga aggtggtatt aaaccagccg
     6901 aagaaaccgc ccttattttc gtgatgctca gcagagacgg gtttaagcag cgttgcacat
     6961 aacgcagggg taagaatcaa tgccaccaga acagaaagtg ccattgccga aacgatggtg
     7021 atagagaact ggcgataaat tgccccagta gaaccgccga agaatgccat cggaataaat
     7081 accgctgaca gcaccatcgc gatacccacc agtgcgccct gaatttgcga catcgatttt
     7141 tccgtcgctt cttttggcgg gagcttatcc tccatcatca cgcgctcgac gttctccacc
     7201 accactatcg catcatcgac gagcagcccg atggcaagca ccatcccgaa catcgttagt
     7261 gtgttgatgg agtaaccaaa agcggcgagg atggcaaacg tccctaacaa caccacgggt
     7321 accgcaatgg tggggatcag cgttgctcgc atattctgca agaacagata catcaccagg
     7381 aacaccagca taatggcttc gaacagcgtt tttaccactt cgtgaataga aagctggacg
     7441 aatggcgtgg tgtcataagg gtagagaacc ttcattccct gcgggaagaa tggctgtaat
     7501 tccgccagtt ttgccttaat ggctttcgcg gtatcgagag cattcgcgcc ggttgccagc
     7561 ttaatcccca ggcccgccgc cggttttccg ttgatacgag cgataacgtt atagttttca
     7621 ccgccaagtt caacccgtgc gacatctttc aggcgtacca ccgagccgtc actgtttacg
     7681 cgcagggtca ctttgccgaa ttcttccgga ttttttaacc gcgtttgagc aataatcgag
     7741 gcattcaact gttgccctgg taacgctggc gttccgccca actgtccggc agcgatctga
     7801 tcgttctgta ccttcaactg gttaatcaca tcaaccggtg tcagtttata tttgtttagc
     7861 agatcggcgt ccagccagat acgcatcgca tactgtgcgc cgaaaagctg tacgtcaccg
     7921 acgccattca gacggctaag cgtatcttta acgttagagg ccacatagtc cgagatatcg
     7981 tcctgtgtgg tgtctgggtt atcagagaca aagcccgcca ccatcaaata gctgctactg
     8041 gacttttcaa cactgatccc ctgctgctga acctcctgcg gcagcaacgg cgtggcgagc
     8101 tggagtttgt tctgcacctg cacttgcgcg atatcaggat cggtcccgga ctggaaggta
     8161 agggtaattg tcacgctacc ggcggaatcg ctggtggagg acatatacat caggttatcg
     8221 ataccattca tattctgttc gataacctgc gtcaccgtat cctgcacggt ctgcgcatca
     8281 gcacctggat agtttgccga aacagaaacc gcaggcggtg caatggttgg atacagagcg
     8341 acgggcaatt gtaggatcgc cagtgcgccc gccatcatca gaataatggc cagcacccat
     8401 gcaaatatcg gtcgtcgaat aaaaaagttt gccatgtcag attaccttac ttcgatgcag
     8461 tatctgcggg ggtatcggta gtggctttaa cctgctctcc cggacgcgct ttttgcaggc
     8521 cgctgacaat gacttgatcg ccagatttca gaccttcgct aatcaaccat ttatcgccaa
     8581 tcgcctgact ggcaacgaca gggcgcgctt ccacctgact tttatcgtta acaatcagca
     8641 cggttgcatc accacgcggt gtgcggctaa cgccttgttg cgggataaga atggcgtcag
     8701 gttggacgcc ttcatcaatc cgtgcacgca caaacatacc cggcaaaagc gtatgttgcg
     8761 ggttagggaa gacagcacgt agggttatgg agccggtgct ttcatcaacg gtcacatcag
     8821 agaattgcag cgtacctttc aggggatagg tttgaccgtt ttccatgacc aactctacgt
     8881 tgctggtggc gttttcctta tgcaaatttc cttgctctac ggattgcttc agcctcataa
     8941 aatcgttgct ggattgggtc acatcaacgt agataggata gagctgctgg acagtcgcca
     9001 gttcagtcgt ttgcccatta gtgacaagag cgccttcggt cacggtcgat ttgccgatac
     9061 gtccgctaat tggcgcagtc actttggtat aagcgagatt gatgcgagcg ctttcgactg
     9121 tggcttttgc ggcaatcacg gcggcatcgg cctggcgagc atcagcaatg gcctggtcgt
     9181 actcctgctg gctgatgtat ttcgtaccca cgagcggaac gtaacgtttt accgtcaaat
     9241 gcgcgatggc ggcggcggct tcacttttcg ccagttcgcc tttcgcgctg tcataatttg
     9301 cctgataggt cgcgggatcg atctggtata gggactggcc tgcttgtaca tcgctgcctt
     9361 cagtgaaatt acgattcagt acgatcccgc taacctgtgg gcgaacttcg gctatacggt
     9421 aagcattggt gcggcctggt aattcagtct taacttctaa cggggccgtt ttcacaatat
     9481 gaacggtaac ctgcggttca ccgacgtgag ctttctcttc tcccttatcg ttacaaccgg
     9541 cgattaaagc cgcggagatc agaataaagg agggcaggag gaaaaacctg gcatgtttcg
     9601 tcattactat tcctcaaaaa accaaaagcg cgttatttac ccagaaaggc aacgcgtt
//
LOCUS       DBKEKI010000152         9629 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.153, whole genome shotgun sequence.
ACCESSION   DBKEKI010000152 DBKEKI010000000
VERSION     DBKEKI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.153"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..1419)
                     /gene="betT"
                     /locus_tag="ACUQPX_003579"
                     /pseudo
     CDS             complement(1..1419)
                     /gene="betT"
                     /locus_tag="ACUQPX_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
     gene            1547..2134
                     /gene="betI"
                     /locus_tag="ACUQPX_003580"
     CDS             1547..2134
                     /gene="betI"
                     /locus_tag="ACUQPX_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HHV9058145.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2148..3620
                     /gene="betB"
                     /locus_tag="ACUQPX_003581"
     CDS             2148..3620
                     /gene="betB"
                     /locus_tag="ACUQPX_003581"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HHV9058146.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            3634..5304
                     /gene="betA"
                     /locus_tag="ACUQPX_003582"
     CDS             3634..5304
                     /gene="betA"
                     /locus_tag="ACUQPX_003582"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HHV9058147.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRANTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALSRSTAGYFVANGMPVRAKK"
     gene            5518..6194
                     /gene="ykgH"
                     /locus_tag="ACUQPX_003583"
                     /pseudo
     CDS             5518..6194
                     /gene="ykgH"
                     /locus_tag="ACUQPX_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
     gene            complement(6437..7132)
                     /locus_tag="ACUQPX_003584"
     CDS             complement(6437..7132)
                     /locus_tag="ACUQPX_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutC/YkgG family protein"
                     /protein_id="HHV9058148.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7125..8552)
                     /locus_tag="ACUQPX_003585"
     CDS             complement(7125..8552)
                     /locus_tag="ACUQPX_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HHV9058149.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(8563..9282)
                     /locus_tag="ACUQPX_003586"
     CDS             complement(8563..9282)
                     /locus_tag="ACUQPX_003586"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HHV9058150.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCIYAVKSYPTYLADEPEWASRAE
                     KVAVRMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         2439 a         2597 c         2396 g         2197 t
ORIGIN      
        1 atcacgaaga aaatcacaaa gctgaacggc agccccatga ttaccgtggt gttttgcagc
       61 gcggatatcc cgttggtcat cagcatgccg agcgtcagca gaccaatcgc caccgaccag
      121 aagacgcgca gccagccggg ggcgtcgctg ttgatatctt taagctgcga ggtgaaattc
      181 cccagcacca gcgcaccgga gtccgccgag gtcacataaa acagcaggcc agtaatggtg
      241 gcgacggagg cgctaaaggt aaacgccgga tactgcgcca gcaggctgta gaagccgcgc
      301 tccggatgga ccatcgcttc ctcggcaaat gccgcgccgc cgtggatgat ttcatacagc
      361 gcgctattgc cgaacaccga gagccataac agcgtgaagg taaacggaat aatcaacgtg
      421 cccagcacga actggcgaat ggtacgccca cgcgagatac gcgccaggaa caagccgaca
      481 aacggcgacc atgccaccca ccatgcccag aagaagagcg tccagttatt catccactca
      541 accggacggt cgaaggcaaa actgttgagc gtcatgccca taaagcgatt cacatagtcg
      601 ccaacattca gcaccagcgc attaagcagg aatgaggtgt cgcccataaa caatacgaac
      661 aggatcaatc ccagcgccag cgcaacgtta agttccgata acacacgaat ccccttatcg
      721 acaccggagg tgacagagat cgtggcgatt atcaccgaca aggcgatcag tgccgctttt
      781 gccgccatag aatcgggaat atcaaacagt acgctcaagc catagttaag ctgcaccaca
      841 ccgataccga gcgtagtggc aataccgaag atggtgccga tcaccgctgc aatatccact
      901 gagtgcccta tcggcccgtt aatccgttta ccgaagatcg ggtacagcgc cgagcggatg
      961 gtgagcggca aattataacg ataactaaag tatccgagcg ccatgcccat cagcgcatac
     1021 atcgaccagc cggttaagcc gtagtgaaac agcgtccaga ccatcgcctg acgcgcggcc
     1081 tcaatcgtct gtcccgcgcc ttccggcggc tgcatatact gcgttaccgg ttcggctacg
     1141 gagaagaaca tcaggtcgat accgatcccg gcagcaaaca gcatcgccgc ccaactcagc
     1201 agactgaatt ccggtttgga ttgttctggc ccgagcttca ccgaaccaaa acgcgaacaa
     1261 gcgatacaga ccacaaagac aatatagagc gttgccgcca gcagatagta ccaaccgaag
     1321 gttttagaaa cccagtccag cgtgcggcca atccacaggg ccgagaagtc gcgaaacagg
     1381 atcgttgtcc ttttccctgc tgtgtgaaag gtccgtcatc cagtatcccc acttttttgt
     1441 taccattaaa tatcaactta gaaacaatta agacacattt tatattgaac gtccaatcaa
     1501 taaccgcttt aatagataaa caacgctgat gaatggagtg gcgaaaatgc ccaaattggg
     1561 gatgcagtcg atccggcgca ggcaactgat cgacgccacg ctggaagcaa tcaatgaggt
     1621 gggcatgcac gatgcaacga tcgcgcagat cgcccgccgt gcaggcgttt ctacggggat
     1681 catcagccac tatttcaggg acaaaaatgg tctgctggaa gcaaccatgc gcgatatcac
     1741 cagtcagctg cgtgacgcgg ttttgaatcg attacatgca cttccgcagg gcagtgcaga
     1801 acaacgatta caggcgattg ttggcggaaa cttcgatgaa acgcaggtga gcagtgcggc
     1861 gatgaaagcc tggctggcgt tctgggccag cagtatgcat cagccgatgc tctatcgctt
     1921 gcagcaggtc agtagccgcc gcttattgtc gaatctggtg agcgaatttc gccgcgagtt
     1981 accgcgccaa caggcacagg aagcgggcta cggccttgcc gcgctgattg acggattatg
     2041 gctgcgcgcg gcactgagcg gcaaaccgct ggataaacct ctcgcacact cgctgacccg
     2101 tcactttatt actcagcatt tacccaccga ttaaccgagg agacgtgatg tcccgaatgg
     2161 cagaacagca gctttatata catggtggtt atacctccgc caccagcggt cgcaccttcg
     2221 agaccattaa cccggccaac ggtaacgtgc tggcgaccgt gcaggccgcc gggcgtgagg
     2281 atgtcgatcg cgccgtgaag agtgcccaac aggggcaaaa aatctgggcg gcgatgaccg
     2341 ctatggagcg ctcgcgtatt ctgcgtcggg ccgttgatat tctgcgtgaa cgcaatgacg
     2401 aactcgcaaa actggaaacc ctcgataccg gaaaagcata ttcggaaacc tcaaccgtcg
     2461 atatcgttac cggtgcggac gtgctggagt actacgccgg gctgatcccg gcgctggaag
     2521 gcagccagat cccgttgcgt gagacgtcat ttgtttatac ccgccgcgaa ccgctgggtg
     2581 tggtggcggg gattggcgca tggaactacc cgattcagat tgccctgtgg aaatccgccc
     2641 cggcgctggc ggcaggcaac gcaatgattt tcaaaccgag cgaagtcacc ccgcttaccg
     2701 cgttaaagct ggctgaaatt tacagcgaag cgggcctgcc ggacggcgta tttaacgtgt
     2761 tgccgggggt gggcgcggag accgggcaat atctgaccga gcatccgggc attgccaaag
     2821 tgtcatttac cggcggtgtc gccagcggca aaaaagtgat ggctaactcg gcggcctctt
     2881 ccctgaaaga agtgaccatg gaactgggcg gtaaatcacc gctgatcgtt ttcgatgatg
     2941 cggatctcga tctcgccgcc gatatcgcca tgatggcaaa cttcttcagc tccggtcagg
     3001 tgtgtaccaa tggcacccgc gtcttcgttc cggcgaaatg caaagccgca tttgagcaga
     3061 aaattctggc gcgcgttgag cgcattcgcg cgggcgacgt tttcgatccg caaactaact
     3121 tcggcccgct ggtcagcttc ccgcatcgcg ataacgtgct gcgctatatc gccaaaggca
     3181 aagaggaagg cgcgcgcgta ctgtgcggcg gcgatgtact gaaaggcgat ggcttcgata
     3241 acggcgcatg ggttgcaccg accgtgttca ccgattgccg cgacgaaatg accatcgtgc
     3301 gcgaagagat cttcggtccg gtgatgtcgc ttctcactta tgaatctgag gacgaagtga
     3361 ttcgccgcgc caatgatacc gactacggcc tggcggcggg catcgtgacg gcggacctga
     3421 accgcgcgca tcgcgtcatt catcagctgg aagcgggtat ttgctggatc aacacctggg
     3481 gcgaatcccc agcagagatg cccgttggcg gctacaaaca ctccggcatt ggtcgcgaga
     3541 acggcgtgat gacgctccag agttacaccc aggtgaagtc catccaggtt gagatggcta
     3601 aattccagtc catattctaa ccaggaggtt tatttgcaat ttgactacat cattattggt
     3661 gccggctcag ccggcaacgt tctcgctacc cgtctgactg aagatccgaa tacctccgtg
     3721 ctgctgcttg aagcgggcgg cccggactat cgctttgact tccgcaccca gatgcccgca
     3781 gcgctggcgt tcccactaca gggcaaacgc tacaactggg cctatgaaac agagcctgaa
     3841 ccgtttatga ataaccgccg catggagtgc ggacgcggta aaggcctggg tggatcgtcg
     3901 ctgatcaacg gcatgtgcta catccgtggc aatgcgctgg atctcgataa ctgggcgcaa
     3961 gaacccggtc tggagaactg gagttatctc gactgcctgc cctactaccg caaggccgag
     4021 actcgcgatg tgggcgagaa cgactaccac ggtggcgatg gcccggtgag cgtcactacc
     4081 tccaaacccg gcgtcaatcc gctgtttgaa gcgatgattg aagcgggcgt gcaggcgggc
     4141 tacccgcgca cggacgatct caacggttat cagcaggaag gttttggtcc gatggatcgc
     4201 accgtcacgc cgcagggccg tcgcgccaat accgcgcgcg gttatctcga tcaggccaaa
     4261 tcgcgcccta acctgaccat tcgtactcac gctatgaccg atcacatcat ttttgacggc
     4321 aaacgcgcgg tgggcgtcga atggctggaa ggcgacagca ccatcccaac ccgcgcaacg
     4381 gccaacaaag aagtgctgtt atgtgcaggc gcgattgcct caccgcagat cctgcaacgc
     4441 tccggcgtcg gcaacgctga actgctggcg gagtttgata ttccgctggt gcatgaatta
     4501 cccggcgtcg gcgaaaatct tcaggatcat ctggagatgt atctgcaata tgagtgcaaa
     4561 gaaccggttt ccctctaccc tgccctgcag tggtggaacc agccgaaaat cggtgcggag
     4621 tggctgtttg gcggcactgg cgttggtgcc agcaaccact ttgaagcagg tggatttatt
     4681 cgcagccgtg aggaatttgc gtggccgaat attcagtacc atttcctgcc agtagcgatt
     4741 aactataacg gctcgaatgc agtgaaagag cacggtttcc agtgccacgt aggatcgatg
     4801 cgctcgccaa gccgtgggca tgtgcggatt aaatcccgcg acccgcacca gcatccggcg
     4861 attctgttta actacatgtc gcacgagcag gactggcagg agttccgcga cgcaattcgc
     4921 atcacccgcg agatcatgca tcaacccgcg ctggatcaat accgtggccg cgaaatcagc
     4981 cccggtgtcg aatgccagac ggatgaacag ctcgatgagt tcgtgcgtaa ccacgccgaa
     5041 accgccttcc atccgtgcgg tacctgcaaa atgggttacg acgagatgtc cgtggttgac
     5101 ggcgaaggcc gcgtacacgg gttagaaggc ctgcgtgtgg tggatgcgtc gattatgccg
     5161 cagattatca ccggcaattt gaacgccacg acgattatga ttggcgagaa aatagcggat
     5221 atgattcgcg ggcaagaagc gctgtcgagg agcacggcgg gatattttgt ggcaaatggg
     5281 atgccggtga gagcgaaaaa atgagtcgtg atttgaacta acgcaggaac cgatcagatt
     5341 gagagttacc gttccagata tggggaccga atcgttatat aaaacactga ggtaactctc
     5401 atgcttcata tccagtattc atgatgctgg ttttttgctg atatttaacc cgaacattcc
     5461 tgatacagac tattaataca acattatctg cgacagattt tattttctgg atatattatg
     5521 agtgaacaaa tcaaacagga tatcgatctg attgagattt tatttcatct gaagaaaaag
     5581 attcgtgtta tcctttttat tatggctatc tgtatggcaa tggtgctgtt gtttctgtat
     5641 atcaatatat caataaagac aatataaaag tgatttacag ccttaaaata aaccagacaa
     5701 cgccaggtat acttgttagc tgtgatagca ataataattt tgcctgtcag accacaatga
     5761 ctgaagatgt tattcagcga attactacat tttttcagac cagcccagat gtcaagaaca
     5821 gagaaataag gctggaatgg tcaggagata agagagattt accaactgct gaagaggaaa
     5881 tatctcgcgt gcaggcgtct attatcaaat ggtatgcgtc agaatactat aatggcaggc
     5941 aagttctcga tgagatacaa acgccttcag caattaacag tgagctttat acaaaaatga
     6001 tatacctgac caggaactgg tcattgtatc cgaacggtga tggctgtgta actataagct
     6061 caccagaaat aaaaaataaa taccctgctg ccatttgcct ggctctggga ttttttctaa
     6121 gcattgtaat ttctgtaatg ttttgccttg tcaaaaaaat ggtagatgaa taccaacaaa
     6181 actctgggca gtaataaaca gcattctgat agagagtgcc tgtataaagc tgaagtgaca
     6241 atgaccggga agggaacccg ttttcaggaa tggatgcctg tctttttcac tgtaaatatc
     6301 ctggcattac taatgccagg atatttacag aagagcagca taaccatgac ggaacggggt
     6361 taagtcaggt tgtccttaac gctattttca acctcgtatg ccttcttcag gtttatgttc
     6421 agatctcata tctctctcag caatcctcaa taatcagata caccgctttc accggaccat
     6481 gaaccccgac gactttgata agctcaatat ccgccgttga gctgggacca ctgatgatgt
     6541 taatacatga cggcattcgt tcaccggcct gcgctttctg atgcaatttt tctgcgagtt
     6601 gcgctacacg cggcaggatt gtgcttttac gcaggataaa aagagaagat tccgggagca
     6661 ggctcaatga acgcccgcgc tcggcggcgg aaaaaagaac cacgcctccc gattcggtta
     6721 aaccatattc agcatacacg acacccactt tagcctgctc tgcctgcgag atattctcgg
     6781 cacctttcgc cggatcccaa acaacggcat tgcattcctg ctgcaaacgt tcactaatcc
     6841 ccaattcctc cagcctcgtg tcaccgctaa tcacgaccga ctgatctccc agctctttac
     6901 acagacgtat tgcagcttct gccgccttcg cctcgctggt cagctcacag cgcgtcaaca
     6961 taacatcgct ggcaaactga ataaacgcgt cacagcgctg ctgttggtta agttgggtaa
     7021 gccgctcgtt agcatagttg ttaagcggcg catcttctgc ttgcggttca agtcgcagcg
     7081 ggcgacccag tgcctgagca acgttattca aaaattcgct ccgattatcc attctttttc
     7141 tcctgcgcct gatgtttctt aaaccaacta cggaaactct ctccgtcagc ttcaggaaga
     7201 tcgcgtgctt ccatccagtc gctaatcgcg ccaaatttga gtggtgtttt gccgccattg
     7261 ataaaccagc ttgccgcatg agcgccggcc atcatcccga ctttccacaa tcctggatga
     7321 ctattggcat aagcgaacat ttttatcgcc cgttgctctg cttttgcggt gatccctttt
     7381 tcagccatca cccgacgatg acgcaaaatc agttttgaca gcggaatacg caccggacac
     7441 acgctgtcac aagcagtgca taaagagcag gcgtagggta aatctttaaa atctttatag
     7501 ccgccaagta gcggagaaat caccgcacca attggccctg gataaataga gccatatcca
     7561 tgaccgccaa tatggcgata tgccggacaa gtattcatac aagccccgca gcgaatacag
     7621 cgcagcacat cccgaaattc agaggccagc acctcagaac gcccgttatc gacaataacc
     7681 agatgaaact cttcaggacc atcaacgtgc cccgcttcgc gcggccctgt cagccaggtg
     7741 ttgtatcccg tcaaacgtgc accaacggca ctgcgcgcca gcatggtgat caatacatct
     7801 acctcggcaa acgtgggggc aatacgctcc attcccatca ctgcaatatg cgttttaggc
     7861 agcgtggtac acattcgcgc attaccttca ttggtcacca ggcataccga accggtctct
     7921 gccaccgcga aattacagcc ggtaatacct atttcagcac tgaggaaatc ttcacggatt
     7981 ttttgccgga tgaataaggt catcgcttca ggcgtttccg gcccctcata gcccagacgt
     8041 tcgtgtagca ctcgacggat ctgatggcga tctttatgaa ttgccgggac cacaacatga
     8101 gatggcggat cttgatccag ctggagaata tattcaccca gatcggtttc aatcacctga
     8161 atgccagcat cctgcaacac atgattgaca ccaatctctt cggtcaccat cgatttagat
     8221 ttcaccacct tccgggcatt tttgcgttgg gcaacctgta aaatgtagcg ggtagcgtct
     8281 tctttggttt ttgcaaaata gacgtgaccg ccgttttgcg tcactttttc tgagagctgg
     8341 tacagataag cgtcgagatt actcagaaca tgatcacgta tctgggcggc ccgatcgcgc
     8401 cactcctccc agtgccccaa ttcatcgacc attttttgcc gatttgcccc aatacgctgc
     8461 tgcgcgtttg ccaccgcttt gcgcatgatc ggatcttcaa tttgctgacg gatgcgtgtc
     8521 ttaaaatctg tattactggt tttgatcgac atctttatat cctcagcggc tcatcaacac
     8581 ttcagcaata tgcatcactt tgactttctg cccttcccgt tgtaatcgcc cactgatgtt
     8641 cagcaggcaa ctcacgtcag caccaattaa atactcaggg cggacttcca tcaggtgcac
     8701 aaccttttct ttcaccatct cgccggatat ttcggccatt ttgaccgaga acgtgccgcc
     8761 aaatccgcag caggtatcct gttcagcaaa ggtcaacagc tccagtccac gcacattttt
     8821 cagcagcgta agtggctcgt ccttcactcc cagcttacgg gccaggctac aagatgggtg
     8881 atacacagct ctcccttgca aactggcacc tacatcgact acccctaatt tattaacaat
     8941 aaaagaggtg agatcctgca tacgcacggc aaccttttcg gcacgtgatg cccattcagg
     9001 ttcatccgcc agatacgtcg ggtaactttt tacggcatag atgcaagagc cagccggtga
     9061 aataatggga tcgtcgttat cctccagtgc ggcgatcaga tttttcatcc ctggaatcgc
     9121 ttctttgata taaccgctat tgatcgcagg ctgaccgcag catccctgtt tctccgggaa
     9181 atttacgcga cagccgagtt tttccagtag cagcacggag tctcgtgcca ttcttgattt
     9241 cagggcgtca ccaatacagg tgacaaagaa attgacattc acaactaata ctccattact
     9301 tcatgcccat ttatgcccga tgtttccccc ccaccggggg atcaatcatc aataaatatg
     9361 gcgttctttt ttaattggct agcaacatac cacccctcat ttgtatgaca aatgataatc
     9421 tttcatccaa acttcaaatg caataactaa ttttatttct ttcggcatcg ctcacatttt
     9481 atggggaaat cgacgatcat ttttggcata agaaatccat ttaaaaatgg ctacatccct
     9541 tatacctatt cagagctact tattcagggg ttattgagat atacactgat ggtgtacaaa
     9601 aaaatcaccg acaataatca cgtatcaag
//
LOCUS       DBKEKI010000153         9509 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.154, whole genome shotgun sequence.
ACCESSION   DBKEKI010000153 DBKEKI010000000
VERSION     DBKEKI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.154"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..197
                     /locus_tag="ACUQPX_003587"
     CDS             <1..197
                     /locus_tag="ACUQPX_003587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058151.1"
                     /translation="VVPLSAALILTTAVLFCLPGAPSAPGCLAASGRQARLRQAAAGA
                     PASLRRTRWRGFGLSLRSDA"
     gene            complement(101..349)
                     /locus_tag="ACUQPX_003588"
     CDS             complement(101..349)
                     /locus_tag="ACUQPX_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058152.1"
                     /translation="MSRGPLQRRRARARNGVAPYIPASFCRDAGSAGHGATEAARPER
                     SLQAGGFHASDRSDSPKPRQRVRRRLAGAPAAACRSRA"
     gene            861..1091
                     /locus_tag="ACUQPX_003589"
     CDS             861..1091
                     /locus_tag="ACUQPX_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058153.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            1143..2504
                     /locus_tag="ACUQPX_003590"
     CDS             1143..2504
                     /locus_tag="ACUQPX_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HHV9058154.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEETAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAETEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            2551..3114
                     /locus_tag="ACUQPX_003591"
     CDS             2551..3114
                     /locus_tag="ACUQPX_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HHV9058155.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            3114..3344
                     /locus_tag="ACUQPX_003592"
     CDS             3114..3344
                     /locus_tag="ACUQPX_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058156.1"
                     /translation="MTQGWREDKCPSVRAGKRPRAGGAERGRAHAPPLSLRGRSDGGF
                     CLFRSVICLLLMMLLFLLLLLLLLLLFFFLPL"
     gene            3266..3514
                     /locus_tag="ACUQPX_003593"
     CDS             3266..3514
                     /locus_tag="ACUQPX_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058157.1"
                     /translation="MSVTDDVVVPATATATATALLFPAALILTTAVLSCLPGAPSAPG
                     CLAASGRQARLRQAAAGAPASLRRTRWRGFGLSLRSDA"
     gene            complement(3511..3685)
                     /locus_tag="ACUQPX_003594"
                     /pseudo
     CDS             complement(3511..3685)
                     /locus_tag="ACUQPX_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3756..3961
                     /locus_tag="ACUQPX_003595"
                     /pseudo
     CDS             3756..3961
                     /locus_tag="ACUQPX_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            3987..4514
                     /locus_tag="ACUQPX_003596"
     CDS             3987..4514
                     /locus_tag="ACUQPX_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HHV9058158.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGTQTQPEEAQQFSGQPQPESQPEPKKGGAKTKGRGRKAAQPEPQ
                     QPSEPAYDFDDDIPF"
     gene            4570..4803
                     /locus_tag="ACUQPX_003597"
     CDS             4570..4803
                     /locus_tag="ACUQPX_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HHV9058159.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNRYIRDFGILK"
     gene            4862..6826
                     /locus_tag="ACUQPX_003598"
     CDS             4862..6826
                     /locus_tag="ACUQPX_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HHV9058160.1"
                     /translation="MPVTKCEPETTRKASRKYAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHSSLTSDAPSGKEGAAFLSMMAEKARL
                     TALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCALRPQAETETTEYRDNQADAA"
     gene            6880..7314
                     /gene="psiB"
                     /locus_tag="ACUQPX_003599"
     CDS             6880..7314
                     /gene="psiB"
                     /locus_tag="ACUQPX_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HHV9058161.1"
                     /translation="MKTELTLNVLQTMNAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVI
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQA"
     gene            7311..8030
                     /locus_tag="ACUQPX_003600"
     CDS             7311..8030
                     /locus_tag="ACUQPX_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HHV9058162.1"
                     /translation="MSARSQALVPLSAEQQATWRAVAETEKRRHQGNLLAEYPYAGAF
                     FRCLNGSRRMSLSDLRFFMPSLTAEELRGNRSQWLYAVDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREVEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLATRE
                     AGHAVREMERWMVPNKLREAA"
     gene            8243..8392
                     /locus_tag="ACUQPX_003601"
                     /pseudo
     CDS             8243..8392
                     /locus_tag="ACUQPX_003601"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            8334..8459
                     /locus_tag="ACUQPX_003602"
     CDS             8334..8459
                     /locus_tag="ACUQPX_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HHV9058163.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            complement(8700..8948)
                     /locus_tag="ACUQPX_003603"
                     /pseudo
     CDS             complement(8700..8948)
                     /locus_tag="ACUQPX_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355714.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9006..9245
                     /locus_tag="ACUQPX_003604"
     CDS             9006..9245
                     /locus_tag="ACUQPX_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058164.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDILEFSNLTELKKYRK
                     SHPEKMAFSYSYSLSRGVDTQFRHINIAEADHFKQ"
     gene            complement(9273..>9509)
                     /locus_tag="ACUQPX_003605"
     CDS             complement(9273..>9509)
                     /locus_tag="ACUQPX_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HHV9058165.1"
                     /translation="RHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMR
                     ATVFNYIECDYNRWRRHSWCGGLSPEQFENLNLA"
BASE COUNT         2108 a         2619 c         2863 g         1919 t
ORIGIN      
        1 ttgttgttcc tttatctgct gctctgattc tgactactgc agtgttgttc tgtctgccgg
       61 gcgcaccttc ggctcccggc tgcctggcgg catccggtcg tcaggcccga ctccgacagg
      121 cagctgctgg cgcacccgcc agtctccgcc gaacccgctg gcgcggtttc gggctgtcgc
      181 tacggtccga tgcgtgaaaa cccccggctt gcagcgaccg ttccggtcgc gccgcctctg
      241 tcgcgccgtg gcctgcgctc ccggcatccc ggcaaaaaga cgccgggatg taaggcgcaa
      301 cgccgtttcg cgcacgcgct cttcgtcgtt gcagcgggcc cctgctcatt gcctgccggg
      361 tgccacacac ccgtatgagt ccctgtcgcc gggggcatag acagccacaa cctgccgcac
      421 tgactgtcca gggtaaatga acagcccaca aaatttttta cccaaaaacc ggtaaaaaat
      481 tttgcgttct gcccaggaca ggtgcgtcag gccgtggcag tgatgcccct tgcggcgaag
      541 gttctcaaac gggtgaagtt cataccacag ggaatacagc agcaggttcc cgcgaaacgt
      601 caaaaccgcg gctcccgaaa ctcaacgggt gatgttcttt aaaaatcagg atttatttca
      661 ggctccggca atacgcattt atgtgttctg tcagtcatcc ggctggcagg taatgagaac
      721 gcagaaacag aaggaggaaa acaccggaga acgtcagacg cagatatgaa aaaagcaggt
      781 cagggacctg cttaatccac accgcaaaaa ctgtttgcgc agtttctgcg gttccattca
      841 acagtcataa ggatgcattt atgacagtaa gtattgtttc accttccgca gcggctgtaa
      901 agccccgccg tcatccccgt ttcagacgcg aagacatccc cgcgccggaa attgaccccg
      961 tgctgaaagc cttcgggcga cacatagccc ggagttttca ccgggggcgt ggtgtacaca
     1021 tccccgccat gaaaaacacg gccttcgggc aggtgctccg cacactcgaa ctgaaacgcg
     1081 cttttaactg atgaggtgag ccgccccgcg aggggcggca acagacggaa ggagaacatt
     1141 ttatgacatt agcagcaatc accatgaccg ccccggaagc cgccagccct gtgcaaatgt
     1201 accgcgcgac ctactcaccg gatgacaaca aactgcgcct gtatgccgcg tcacgtcttg
     1261 acccggagac gtataaaaaa gtgcatgatg ccggttttcg ctgggcaccc aaacaggcgc
     1321 tgtttgtcgc gccagcctgg acaccgggcc gggaagacgt gctcctctca cttgccggag
     1381 agattgagga tgaagacagc acgctcgctg aacgtcagga agcacgggcg gagcggttta
     1441 ccggatacag cggaaagcgg gccagtgaat ccgcacaggc acttgatgaa gtggaaagac
     1501 tggccgcgat gatcccgccc ggtcagccca ttcttgtggg gcatcacagc gaacgccgcg
     1561 cccgtcgtga tgcacagcgt attgaaaacg gtatgaaacg tgctgtgatg ctctttgaac
     1621 gtgcggaata ctgggaagag cgggcgcggt cggcactgct tcacgcgaag tataaagaac
     1681 gtccggacgt tcgctggcgt cgtatcaaaa aaatcgaagc tgatttgcgc aaggcggaaa
     1741 agaccatcgc acagtcgcag aaatatctga cgatgtggcg ggctgaatcg ctggatctga
     1801 atatggcaaa actcatcagc agtcatgacc atatcagcgc ctgtttcccg ctggatacgt
     1861 atccgcgccc ggcagaaaaa agccagtatg aaggaagtcg gtcgttatgg tcggccctgg
     1921 atgatgacat catcaccacg gagcaggccc gcgaaattgc gatccgctgt catgaacggc
     1981 agattcagca tcagcaacgc tgggttaacc actatcagaa ccgcctgatc tatgagcgtg
     2041 ccatgctgga cgaaagcggc ggcgtggtta cccggacaca ggattttgag ccgggcggac
     2101 aggttttcag ccggggcgag tggctgacca tcatccgcgt gaacaaaagc aacggggcgg
     2161 tgagttcagt cacaacgcca aattacagtt ttctcgggta cagcggcacg atgaaagtga
     2221 cgcccgatcg catcacggac tacaaagcac catcggcaga agagactgcc gtcgccagtc
     2281 aggccgcgaa gcgtccgccg gtagtcaact atccggggga aggtttccgg gaaatgacaa
     2341 aggcgcagtg ggccgccctg ccccgggact gtaaggccgt gcgcagtgtg gcagagacag
     2401 aagaccacgg ggcataccgc taccgccgca caatggacaa taatttccgc ctggtgaatg
     2461 tgtatattac cgacatgaaa atcacggaaa tcccacagaa ataaggtata tccccccggg
     2521 aaatccccgg gggatgacgg agataatgac atgcacagtc agttaagaga acgtatccgg
     2581 ctgatgcgcg caaggctgga taacgccgcg ccggttgctg aaatccgggc tgaatctcag
     2641 ctttttgtga ctcctgcccc ggtctgtgat cgcctggtga cgctggcgga aatcagcaac
     2701 cgtgatcaca ttctggaacc ctctgccggc accggggcca tactgcgggc gattcgggat
     2761 accgcgccgg aggccatgtg tgatgcggtg gagattaaca gcgggctggt ccggtatctg
     2821 cgggaaaatt ttaacggtgt cagggtacag tgtggcgact tcatggaatg gcagccggtg
     2881 cagtattaca gccggatcat tatgaatccg ccgttcagtc acgggcagga tatccggcat
     2941 atcctgcggg ctttttccct gttgcgtccg gggggcgttc tggttgccgt ctgtctcaac
     3001 gggccacgcc agcaggagaa gctgttaccg ttttctgacg tccgcgagga gctgccgcgc
     3061 ggcacgtttg cttataccga tgtcccgacg atgattattc gtttgcgtgc ctgatgacac
     3121 agggctggcg ggaggataaa tgcccgtctg tccgcgccgg gaaaagaccg cgcgccggtg
     3181 gcgctgaaag gggccgcgct cacgcgccgc ccctttcatt acgcggacgt tctgatgggg
     3241 gcttctgtct tttcagatct gtcatttgtc tgttactgat gatgttgttg ttcctgctac
     3301 tgctactgct actgctactg ctcttctttt tcctgccgct ctgattctga ctactgcagt
     3361 gttgtcctgt ctgccgggcg caccttcggc tcccggctgc ctggcggcat ccggtcgtca
     3421 ggcccgactc cgacaggcag ctgctggcgc acccgccagt ctccgccgaa cccgctggcg
     3481 cggtttcggg ctgtcgctac ggtccgatgc gtgaaaaccc cgggcttgca gcgaccgttc
     3541 cggtcgcgcc gcctctgtcg cgccgtggcc tgcgctccct gcatcccggc aaaaggccgc
     3601 cgggatgtaa ggcgcaacgc cgtttcgcaa accgcgcttc acgtcgttgc ggcgggtccc
     3661 tgcccgttgt tctgtccggt gccattcacc tgtttaagcc cctgtcgccg ggggctgata
     3721 cagccacaac ctgccgcact gactgtccag ggtaaatgaa cagcccacaa aattttttac
     3781 ccaaaacccg gtaaaaaatt ttgcgttctg cccaggacag gtgcgtcagg ccgtggcagt
     3841 gatgcccctt gcggcgaagg ttctcaaaca ggtgatggtg aataccggag ggaacaaaag
     3901 gcaggtcccc gcaaaacgtc aaaaccgcgg ctcccgaaac tcaacagatg atgggggctg
     3961 aaaacataaa cagaaggaga cacatcatgg cagttcgtgg cattaacaag gtcattcttg
     4021 tcggtcgtct gggaaaagat ccggaagtcc gttacatccc aaacgggggc gcagtggcaa
     4081 acctgcaggt ggccacgtca gaaagctggc gtgacaaaca gacgggggag atgcgggagc
     4141 agacagaatg gcatcgcgtg gtgctgttcg gcaagctcgc ggaagtggct ggtgagtatc
     4201 tgcgcaaggg cgcgcaggtc tacatcgagg ggcaactccg tacccgtagc tgggaagata
     4261 acggtatcac ccgttacgtc actgaaattc ttgttaagac cacgggcacc atgcagatgc
     4321 tgggacgtgc cgcaggtact cagactcagc cggaagaggc gcaacagttc agcggtcagc
     4381 ctcagccgga atcacagccg gaaccgaaaa aaggtggcgc gaaaacgaaa ggccgtggac
     4441 gtaaggccgc gcagcccgag cctcagcagc cgtctgaacc cgcgtatgac ttcgatgacg
     4501 acattccgtt ctaacgaaag acagtgacaa ccgccccgtc cagtgcgggg catcaccgga
     4561 gagatgagga tgagcgaata tttcagaata tttcaggggc tgcctgacgg ctcctttacc
     4621 cgcgaacagg ctgaagccgt tgccgcacag taccggaatg tctttatcga ggatgatcag
     4681 ggaacgcatt ttcgcctggt tgtccgtcag gatggcacgt tgatctggcg ctcctggaat
     4741 tttgaggacg gtgccgggta ctggatgaac cggtacatca gggatttcgg gattcttaag
     4801 taagagaggt gccggacgcg cgaacgtccg gcaggacata agcaattata aggggatgat
     4861 tatgcctgta acgaagtgtg aaccagaaac cacccgcaaa gcaagccgta aatatgcaaa
     4921 aacgcaggaa acagtcctgt ctgccctgct ggcgcagacg gaggaagtga gtgtgccgct
     4981 ggcttcactg attaaatcac cgctgaatgt gcgcacggtg ccgtattctg cggagtccgt
     5041 cagcgaactg gcggactcca ttaaaggtgt cggcctgtta cagaatctgg ttgttcatgc
     5101 cctgccaggt gaccgttacg gtgtcgccgc aggcggtcgc cgactggcag cactcaacat
     5161 gctggcagag cgcaacatcc ttccggctga ctggcctgta cgcgtgaagg tcattccgca
     5221 ggagctggcg actgccgcat cgatgaccga gaacggtcag cgtcgggata tgcaccctgc
     5281 cgaacagatt gccggatttc gtgcaatggc gcaggaaggc aaaacgcctg cacaaatcgg
     5341 tgatttgctg ggctattcgc cccgccacgt tcagcgaatg ctgaaactgg cagaccttgc
     5401 gcctgtcatc ctcgatgcgc tggcagaaga ccgcatcacc acagagcact gtcaggcgct
     5461 ggcgctggag aacgacaccg cgcgtcaggt gcaggtgttt gaagccgcct gccagtcggg
     5521 atggggcggt aaaccggaag tgcagaccat tcgtcgtctg gtgaccgaaa gtgaagtggc
     5581 ggtggcgggg aacagtaaat tccgcttcgt gggggctgat gccttctcgc cagacgaact
     5641 gcgcaccgat ttgttcagcg acgacgaggg tggctatgtg gactgcgtgg cgctcgatgc
     5701 cgccctgctg gaaaaactcc aggctgtcgc agagttcctt cgggaagccg aaggctggga
     5761 atggtgcgcc ggacgcatgg agcctgtcgg tgagtgccgt gaggatgccg gaacataccg
     5821 ctgtctgccg gagccggaag cggtgctgac ggaggcggaa gaagaacgtc tgaacgaact
     5881 gatgacgcgt tacgacgcgc tggaaaacca gtgtgaggaa tctgacctgc tggaagcaga
     5941 aatgaagctg atgcgctgca tggcgaaggt cagagcgtgg acgccggaga tgcgtgccgg
     6001 aggtggcgtg gtggtgtcct ggcgttatgg caacgtgtgt gtccagcgtg gtgtgcagtt
     6061 gcgcagtgag gatgatgcgg ctgacgacgc tgaccgcacg gaacaggtgc aggaaaaagc
     6121 gtcagtggag gaaatcagtc tgccactgct gacgaaaatg tcttccgaac gcacgctggc
     6181 agtccaggcg gcactgatgc agcaaccgga caaatccctg acactgctgg catggacgct
     6241 ctgcctgaat gtgtttggca gcggagcgta cagtaaacca gcacaaatca gcctggaatg
     6301 taaacattct tcgctgacca gcgatgcgcc gtcagggaag gaaggagccg cattcctgtc
     6361 gatgatggca gaaaaagccc gtcttactgc cctgctgccg gagggatggt cacgggacat
     6421 gacgacgttc ctgtcactca gccaggaggt gctgttatcc ctgctcagct tctgtaccgc
     6481 gtgcagtctt aacggagtcc agacccgcga gtgtggtcac acgtcacgca gcccactgga
     6541 cacgctggag agcgccatcg gttttcacat gcgcgactgg tggcagccga caaaagcaaa
     6601 cttcttcgga cacctgaaaa agccgcagat tatcgcagcc ctgaatgatg ccggactgtc
     6661 cggtgccgca cgggacgcag agaagatgaa gaaaggtgat gcggctgaac atgcagagtt
     6721 ccatatgaaa gacaaccgct gggtgcctgg ctggatgtgt gcactacgtc cacaggcaga
     6781 gacggaaacc acggaatacc gtgacaacca ggccgatgcc gcctgatgaa caaccacacc
     6841 gcccgccgga gacggggcgg cagcaaggga gataccgtga tgaaaactga actgaccctg
     6901 aatgtattac agaccatgaa cgcacaggaa tatgaagata tccgggctgc cggaagtgat
     6961 gaacgccgtg agctgactca cgccgtaatg cgggagctgg atgcgccgga taactggacg
     7021 atgaacggcg agtacggcag cgagtttggc ggctttttcc ccgtccaggt ccgtttcacg
     7081 cctgcccacg aacgttttca cctggcgtta tgttcaccgg gcgatgtctc tcaggtctgg
     7141 gtgctggttc tggtgaatgc cggtggtgag ccgtttgcgg tgattcaggt acagcggcgg
     7201 tttgcaccgg aagccgtcag ccattcactg gcactggcgg cgtcactgga tacgcagggg
     7261 tacagcgtta acgacatcat ccatattctg atggcggaag gaggtcaggc atgagcgcac
     7321 gttcacaggc actggttccc ctcagcgcag agcaacaggc cacatggcgg gcggtggcgg
     7381 agacggaaaa acgccgccat cagggaaact tgctggcgga atatccgtat gcgggggcgt
     7441 ttttccgctg tctgaacgga agccgcagga tgtcgttgtc tgacctgcga tttttcatgc
     7501 cgtccctgac cgcagaagaa ctgcgcggca accgttctca gtggctgtat gcagtcgatg
     7561 tgctgattga aactcagggc gaggtctgcc ttcttcccct tcccggcgat gcagcagagc
     7621 ggctgttccc gtcggtgcgg ttccgtgtca gggagcgcag tcgccacaaa tccgcactgg
     7681 tcatgcagaa gtacagccgt cagcaggcac gggaggtaga gcagaaagcc cgtgcgtacc
     7741 aggcgctggt cgctcaggcg gaaatcgaac tggcgttcca ttcaccggaa acagtcggaa
     7801 gctggcatgc ccgctggagt gacagggtgg cagagcatga tctggaaacc ctgttctggc
     7861 agtggggcga gcgttttccg tcactggccg gaatggaacg ctggcagtgg caggatatgc
     7921 cattctggca ggttattgcg gaagccagtc ttgcaacaag agaggctggt cacgctgtcc
     7981 gtgagatgga gcggtggatg gtgccgaaca aactccggga ggcagcgtga tgcggcaata
     8041 atcacacgga ttacccgtag cctgaatgag cgggttattt tcagtaaaag tgagtgtggt
     8101 cagcgtgcag gtatatgggc tatgatgtgc ccggcgcttg aggctttctg cctcatgacg
     8161 tgaaggtggt ttgttgccgt gttgtgtggc agaaggacaa aagccccgta gttaattttt
     8221 cattaaccca cgaggcccct gcatgcttag acaacatcag gatagcctct taccgcgctt
     8281 tgcgcaagga gaagaaggcc atgaaactac cacgcagctc tcttgtctgg tgtgtgttga
     8341 tcgtgtgtct cacactgttg atattcactt atctgacacg aaaatcgctg tgcgagattc
     8401 gttacagaga cggatacagg gaggtggcgg ctttcatggc ttacgaatcc ggtaagtagc
     8461 aacctggagg cgggcgcagg cccgcctttt caggtctgat gctggtctga ctgcactgaa
     8521 gcgcctttat aaaggggctg ctggttcgcc ggtggcccct ttctccttgc tggcattgta
     8581 cgggcatgaa caaccagact gccaggaagg ccgtttccct tcgggaacgg ggatgaacgt
     8641 gcgcctgcgg cgcccgttct tttcccccgc cttctctgtt tatgacctgt cggaaaccac
     8701 atcaccttcc ctgtgattat tctctttttc ctcctgttct gactctgatt actgctgccc
     8761 tgattcctgt ctgtcttgtt cttcttctgc ctgtgccttt cccgggcgtc cccctggcgg
     8821 ggcgggctgt cgtgaacgga gccagcggag ctgtctccgc cctggcggac ttccatccat
     8881 gacgcgaaac cccgggcttg cagcgaccgt tccggtcgcg ccgcctctgt cgcgctgtgg
     8941 cctgcggcgg ctcccgaaac tcaaccgatg atgggggctg aaaaccagag agaggagaaa
     9001 gcaggatgag tacccgaaat atccacgtca acaccgcgtc gtacacgctt cttgttgccg
     9061 ggaagaaaaa aaacaccggg gaagagtggg acattctgga attcagcaac ctgactgagc
     9121 tgaaaaaata tcgcaaaagc cacccggaaa agatggcctt cagctatagc tactcgctca
     9181 gccggggtgt ggatacgcag ttccgccata tcaacattgc ggaagccgat cacttcaaac
     9241 agtgatccta cccacgtaat atggacacag gcctaagcga ggttcaggtt ttcaaattgt
     9301 tccggactga ggccgccaca ccaactgtgc cgccgccacc gattgtaatc acattcgata
     9361 taattaaata ccgttgcccg cattatttcc cggctgataa agtgttctcc atggatacat
     9421 tccactttca gcgaatgaaa gaagctttcc acgcaggcat tatcgtagca gcaacctttt
     9481 gcgctcatac ttccacgcag attatgccg
//
LOCUS       DBKEKI010000154         9467 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.155, whole genome shotgun sequence.
ACCESSION   DBKEKI010000154 DBKEKI010000000
VERSION     DBKEKI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.155"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            243..1742
                     /gene="pitB"
                     /locus_tag="ACUQPX_003606"
     CDS             243..1742
                     /gene="pitB"
                     /locus_tag="ACUQPX_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HHV9058166.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1791..2483)
                     /locus_tag="ACUQPX_003607"
     CDS             complement(1791..2483)
                     /locus_tag="ACUQPX_003607"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HHV9058167.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNHVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2657..3370
                     /locus_tag="ACUQPX_003608"
     CDS             2657..3370
                     /locus_tag="ACUQPX_003608"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HHV9058168.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3402..4160
                     /locus_tag="ACUQPX_003609"
     CDS             3402..4160
                     /locus_tag="ACUQPX_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058169.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4206..5555
                     /locus_tag="ACUQPX_003610"
     CDS             4206..5555
                     /locus_tag="ACUQPX_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HHV9058170.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5555..6375
                     /locus_tag="ACUQPX_003611"
                     /pseudo
     CDS             5555..6375
                     /locus_tag="ACUQPX_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6387..6946
                     /locus_tag="ACUQPX_003612"
                     /pseudo
     CDS             6387..6946
                     /locus_tag="ACUQPX_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
     gene            6977..8047
                     /gene="lptF"
                     /locus_tag="ACUQPX_003613"
     CDS             6977..8047
                     /gene="lptF"
                     /locus_tag="ACUQPX_003613"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HHV9058171.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            8044..9035
                     /locus_tag="ACUQPX_003614"
                     /pseudo
     CDS             8044..9035
                     /locus_tag="ACUQPX_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
     gene            complement(9071..>9467)
                     /locus_tag="ACUQPX_003615"
     CDS             complement(9071..>9467)
                     /locus_tag="ACUQPX_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HHV9058172.1"
                     /translation="EVLRYGSRPYIFTASPSPSCIATVRSSLRTIATQPELRQKLMDN
                     ANHLYDGLQKLGYELSSHISPVVPVIIGSKEEGLRIWRELISLGVYVNLILPPAAPAG
                     ITLLRCSVNAAHSREQIDAIIQAFATLKQ"
BASE COUNT         2224 a         2244 c         2471 g         2528 t
ORIGIN      
        1 gattaatcaa aatattcaat atttatatat tccaatattt attcatttca tatgtgaata
       61 tattaaccag tggaatacct gtgttttgat tatttctaaa ggttttgaat gaatgcttat
      121 tgtctgatac acaagaaata acactctttt tatcgttaaa aaatgatatt tcactttgcc
      181 catgccgtta aagtcactga taatgcgtcc gttcgtaaat tcaaaatggc gtaatctaat
      241 atatgctaaa tttatttgtt ggccttgata tatatacggg gcttttgtta ttacttgctc
      301 tggcatttgt gttgttctac gaagcaatca atggttttca tgacacggcg aatgcggtgg
      361 caaccgttat ttatactcgt gccatgcagc cacaacttgc tgtggtgatg gcggcatttt
      421 ttaacttttt tggcgtgtta ttgggcggac ttagcgttgc ctatgccatt gtccatatgt
      481 taccaaccga tttgttgctg aatatggggt caactcacgg tctggcgatg gtcttttcca
      541 tgctgctggc ggcgattatc tggaacctgg gaacgtggtt ctttggttta ccggcctcca
      601 gttcgcacac cttgattggt gcgattatcg gcatcggttt aaccaacgcg ctgttaaccg
      661 gctcatcggt gatggatgcg ttaaacctgc gtgaagtaac caaaattttc tcctcgctga
      721 ttgtttcccc tatcgtcggc ctggtcattg cgggtggtct gattttcctg ctgcgccgtt
      781 tctggagtgg gacgaaaaag cgtgaccgta ttcaccgcat tccggaagat cgcaaaaaga
      841 aaaagggcaa acgtaaaccg ccattctgga cgcgtattgc gctgattgtt tccgctgcgg
      901 gcgtggcgtt ttcgcacggc gcgaacgacg ggcagaaagg gatcggcctg gtaatgctgg
      961 tactggtggg gattgcccct gctggcttcg tcgtcaatat gaatgcgtcc ggctatgaaa
     1021 ttacccgtac ccgcgatgcc gttaccaact tcgaacacta cctgcaacag catcctgaac
     1081 tgccgcagaa gttgattgcg atggaacctc cattgcctgc agcatcgact gatggcacgc
     1141 aagtaacaga gtttcactgt catccggcaa atacctttga tgcgattgcg cgcgttaaaa
     1201 cgatgctgcc aggcaatatg gaaagttacg agccgttaag cgtgagtcag cgcagccagc
     1261 tgcgccgcat tatgctgtgc atctctgata cctccgcgaa gctggcgaaa ctgccaggcg
     1321 tcagtaaaga agaccagaac ctgctgaaaa aactgcgcag cgatatgtta agcaccattg
     1381 agtacgctcc ggtgtggatc atcatggcgg tagcattggc gctcggcatt ggcaccatga
     1441 ttggctggcg tcgtgtagcg atgaccatcg gtgagaagat tggtaagcgc ggcatgacgt
     1501 atgcgcaagg catggcggca caaatgacgg cggcagtgtc tatcggcctt gccagttata
     1561 ttgggatgcc tgtctccaca acacacgtcc tctcgtctgc ggtagcaggg acgatggtgg
     1621 tggacggcgg tgggttacag cgtaaaacgg taaccagcat cctgatggcg tgggtgttta
     1681 ctttaccggc ggcaattttt ctttctggtg ggctgtactg gatagcattg cagttgattt
     1741 aaccttcctg aaaatgcccg gtcctggcga tcgggcattt ccatttttga ctagtgataa
     1801 ccacgcgcgg tcataaaatc cgtaatcgct ttttctgcat caaccaacac ctgttccagt
     1861 ggctgattgg catcgatatc aaccagttgt gcaccaccaa aggttaactg tggcgttatg
     1921 gcaatcttcc tcgccagcga ttcccgttta tggtcgggtt tacgtgcaca ggcaacttca
     1981 aggtcaacat tgagtttgat gaccagatca ggcttatggc tcgccatcca gtgaaacgct
     2041 ttacgttcct ggcttgccag ccatgagaca aaacgaccac cttcaacgtt aggtgggaac
     2101 accgtaccat cgtaagcgcc aggaatttgg tcctgaggat aacggtcggt tagaacaatt
     2161 aacccgcgac gacgacaggc aagcatatga cgaaagcgca gtaagcgacg ggcgacaaac
     2221 gctgtaatta ccagcgccgg aactggtcca ggcaattttt ttgctgtttt cacatgattt
     2281 cgttcaattg ttttatgtaa ggattttccc atcaaaggta attttgtcac tgcacgaccg
     2341 acatttccag cctgttttcc taaatgaact ctttcggcag caccatattt ttcgacaacg
     2401 gtaataagat gttcacacac cgttgacttg cctgaaccat cgctaccaat aacggcaatt
     2461 aatggaggtg ttattgactg catgaatata tcctttaatt taaatatcca ttaaaaatat
     2521 ttatttggtt aatatgtttt tatgaaagcg taattcaggt caatgtcaca attaaccatt
     2581 gtcacaataa ggttgaacgg atattctgag cacggaccac gtaatattca atacattatt
     2641 tactgccggg tttttcgtga atcaacgtaa tatgtcaata attaattcca cgccagtgcg
     2701 tgttattgcc attgtaggat gtgatggttc aggtaaatcg accctcacgg caagcctggt
     2761 aaatgaactg gcagcaagaa tgccaacaga acacatttat ctcgggcaat cgtccgggcg
     2821 aattggcgaa tggatttcac agctccctgt tattggcgca ccttgtgggt gttatctgcg
     2881 aagtaaagcg gcacatgtac acgaaaagcc ctcaacaccg cctggcaata ttactgcgct
     2941 ggttatctat ctgctttcct gctggcgggc gtacaagttt cgcaaaatgt tgtgtaaaag
     3001 ccagcaaggc tatctgctca tcaccgaccg ctacccgcag gttgaagtgc cgggttttcg
     3061 ctttgatggc ccgcaattgg caaaaaccac gggcggtaac ggttggataa aaatgttaag
     3121 gcagcgcgag ttgaagctgt accaatggat ggcgtcttat ttacccgtat tgttgattcg
     3181 tcttggcatt gatgaacaaa ccgcgtttgc gcgtaaacct gaccaccaac tggcagcgtt
     3241 acaggaaaaa atcgccgtta cgccgcaact gacattcaat ggcgccaaga tccttgaact
     3301 ggatggtcgg caaccagccg atgaaattat gcaagcgtca ctacgcgcaa ttcacgccgc
     3361 gctttcctga tcaccaaaat ggatagggga tgatttaacg aatggatgca ctacaaacac
     3421 aaactgttaa tagtacaacc gcgccgcagc ccaactacat tccggggctg attgcggtgg
     3481 tcgggtgtga tggcaccggt aaatccacgc tgaccaccga cctggtgaaa tcgctgcaac
     3541 aacactggca aaccgagcgc cgctatctgg ggctgctctc cggcgaagac ggcgacaaaa
     3601 tcaaacgatt gccgttggtt ggcgtctggc tggaacggcg actggcggcc aaatcctcga
     3661 aaacccaaag catgaaaacc aaatctccgg cgctatgggc ggcggtgatt atgtactgct
     3721 tctcgctgcg aagaatggcg aatctacgca aggttcagcg actggcgcaa agtggcgttc
     3781 tggtggtcag cgatcgcttc ccgcaggctg aaatttcggg cttttattat gacggaccgg
     3841 ggatcggcgt cgaacgtgcg accgggaaaa tcagcatgtt tctggcgcag cgcgaacggc
     3901 gtttatacca aaaaatggcg caataccgtc ctgaattaat cattcgtttg gggattgata
     3961 ttgatactgc catctcccgc aagcctgacc atgagtatgc cgagctgcag gacaaaatcg
     4021 gcgtcatgtc gacgattggc tataacggca caaaaattct tgagattgat tccagagcgc
     4081 cctacagcga agtgctggaa caggcacaga aggcggtttc tctggttgcc atcgtttctg
     4141 accgccgaag tttaacttaa ggctggcggg atatttttta ctatcaacga taaggtcagg
     4201 ctgaattggc gggttttaac atcaaacatt ggtttgcaga tggcgcgttt cgcaccatta
     4261 ttcgcaatag cgcctggtta ggctccagta atgtcgagag cgccttgctg gggctgttgg
     4321 cgctctcgtg tgccggtaaa gggatgacgc ccgccatgtt tggcgtactg gtgattgtgc
     4381 aatcgtacgc caagtcgatc agcgatttta ttaagtttca gacatggcag ctggtggttc
     4441 agtacggaac gccagccttg accaacaata atccgcagca atttcgcaat gtcgtctcat
     4501 tttccttctc gctggatatc gttagcggcg cggtggcgat tgtcggcggc attgccttac
     4561 tgccattcct ttcccattca ttaggtctgg atgaccaaag cttttggctg gcggcgctgt
     4621 attgcacgct cattccttca atggcttcct ccacgccgac cggcattctg cgtgcggtag
     4681 atcgctttga tttgattgct gtacagcagg cgacgaaacc ttttctgcgc gcagcgggga
     4741 gcgtcgcagc ctggtatttt gactttggtt ttgcgggttt tgttattgcc tggtacgtgt
     4801 cgaatctggt tggcggcacc atgtactggt ggtttgccgc gcgcgaatta cgccgtagaa
     4861 atatccataa cgccttcaaa ttgaatctgt ttgagtctgc ccgacacatt aaaggcgcgt
     4921 ggagttttgt ctggtcaacc aacattgccc actccatctg gtcggcgcgt aactcgtgca
     4981 gcacagtgtt ggtggggatc gtgttaggac ccgctgccgc cgggttattt aaaatcgcca
     5041 tgacattctt cgacgccgcc ggaacgccag cgggtttgct gggtaaaagt ttttacccag
     5101 aggtgatgcg tttagatccg cgcaccacca gaccgtggtt gttaggtgtg aagtccggtt
     5161 tactggcggg cggaatcggt attctggtgg cgctcgcggt gttgatcgtc ggcaagccgc
     5221 tcatttcgct ggtgtttggc gttaagtatc tcgaagcgta tgacctgatt caggtgatgc
     5281 tgggcgcaat tgtgatttcg atgctgggct tcccgcagga gtcattgcta ttgatggcgg
     5341 gaaaacagcg cgcttttctc gtggcgcaaa ccatcgcctc aatcggctac atcgtattgc
     5401 tgttcatgtt ctgtcatctg tttggtgtgc tgggtgcagc gtttgcttac ttcggtgggc
     5461 aatgtctgga cgtggcgctc tcgttaattc caaccttaaa ggcatttttt cagcgccatt
     5521 ccttgcttta taacgcagcc ggagagaaat cctgatgatg agaaaatatt tcccgcttga
     5581 ggcgagcgtc tatttgttgc gattgaagaa gatgatgtcg ttgatgcaca ggttagtttg
     5641 ccgccgacta ttgcactaag ttgtaccacc gaaatcattc atgataatta cgcgttatgc
     5701 ctgcaattct ggctcaatgg cgttgatcgt caggaattac tgcgtttagt tcgcaaacaa
     5761 gccaaaggcg atgagctgac tgctgatgag cgcaaacaat ttaaatacat gcgagcgcgc
     5821 tacaagcacc tgcgttttgc gcaacggctg tatttaaaga agcatcaggc cggattttta
     5881 ttcggcaaaa cgaccgtttt tctggggcgt tttcaggacg gctttcgtaa cggtaagaaa
     5941 aatattgtgt cgtattacgg caacctgtta cggatttatt taagctctcc ggtctggtcg
     6001 ttggtgaact attcattacg ccatagccag ttagaaagcg taagcagttt tattgcctat
     6061 cggcaaaagc aaatgcatac cctgaaagag attattgcca aaccgcgact aaccggcagg
     6121 gaatttcatg atgtgcgaaa aatcatcagc cagcaggttt cttattacga caccttaaga
     6181 tcgcttgatc ctgaaaataa agaggcgtta cagatttccc gtttcctcgc cgccattaat
     6241 ggcctgatgg gagataaaca cgacgacatg gtggcggatg atatggaaaa ccgccagtcg
     6301 tatgatgcac cgttggcgct ggatagcgat attcgtcagc gcttagagct gctgatttcg
     6361 cgttttccgc tatagccgga gcaatgatgt catctcgttt gattattgcg ttgattatca
     6421 tgctcttagc cccgggtgtg caggcacaca atttcgtcac tggcaaaacg gttacccctg
     6481 tttatatcca ggaaggcggc gagctgcttc tgaatagcga cgacgaaatt cactaccaaa
     6541 agtggaacag cacacaatta gcgggaaaag tccgcattat tcagtatatc gccgggcgta
     6601 agtcggcgaa gaaaaagaac tcactgttaa ttaaagcggt tgaagcggca aactttccgc
     6661 aggatcgttt tcagcccacc accattgtga acaccgatga tgccatcttt ggtactggct
     6721 attttgttgt tggcaaaatc gaaaaaaata aacgcgctat ccgtgggcgc aattcgttat
     6781 tgacggcaat ggacagggac gcgttgcctg gcgcttgccg gagcagagtt caaccattct
     6841 ggtgctgaat aaagccgggc aaattcagtg ggcaaaggac ggcagcctga cgccggaaga
     6901 ggtagatcat gtcattgcgt tggcgcagaa actgatcaat gaataaaaaa ctcaaaggat
     6961 tgagggcgtt agtgagatga agctggttga acactacatc atgcgtggta cgcgccgtct
     7021 ggtgctgatt atcgtcggtt ttctgatctt tattttcgcc agctactccg cacagcgtta
     7081 tctgaccgaa gcggcaaatg ggacattagc tcttgatgtt gtgctggata tcgttttcta
     7141 caaagtactg attgcactgg agatgttgtt acctgttggt ctgtatgtgt cagttggcgt
     7201 gacgctaggg cagatgtata ccgactcgga aattaccgct atctctgcgg cggggggcag
     7261 tccgggacgc ttgtacaaag ccgttcttta tctggcgata ccgctaagta tttttgtcac
     7321 ccttctgtcg atgtatggtc gtccgtgggc ttatgcgcag atttatcaac tggagcagca
     7381 gtcgcagtcg gagctggatg ttcgccagtt gcgggcaaag aaatttaaca ctaacgataa
     7441 cggacgaatg atcctttcgc agacggttga tcaggataat aatcgcctga ctgacgcgct
     7501 gatttatact tctactgcca atcgaacccg tattttccgc gcccgttcgg ttgatgtggt
     7561 tgacccatca cccgagaaac cgaccgttat gttgcacaac ggcactgcct atcttctcga
     7621 tcatcagggg cgtgacgaca acgaacagat ctaccgtaat ctgcaattac atctgaatcc
     7681 gctggatcaa agcccgaacg tcaaacgcaa agcgaaatcg gtcacggagc tggcgcgctc
     7741 cgcctttcct gccgatcatg ccgaactgca atggcgacaa agccgtggcc tgaccgcatt
     7801 gttgatggcg ctgctggcca tttcattaag tcgggtaaaa ccgcggcaag ggcgattttc
     7861 aacgttattg ccactgacgt tgctgtttat tgccattttt tatggcggcg acgtctgccg
     7921 tacactggtg gctaacggcg cgattcccct cattcctggt ttgtggttag tgcccggact
     7981 catgctaatg ggcctgctga tactggtcgc acgtgacttc tctttgctgc agaaattttc
     8041 ccgatgaatg ttttcagtcg ctatttaatc cgtcatctct ttctcggttt tgccgccgct
     8101 gcagggctat tgctgccgct ttttaccacc ttcaacctga ttaacgaact ggatggtgtc
     8161 agcccgggcg gttatcgctg gactcaggcg gtgctggtgg tgctaatgac cttaccgcca
     8221 ttcgcagcac ggggttttct atcttccgta ttgcactggt ggcgctggtt gccgggatat
     8281 tgtggactgt ttcgttaggc gcgatagatg agtgggtggc gtcgccattg cagcaacagg
     8341 cgttgcaaat caaatcgacc gccaccgcgt tgggggagga cgatgacatt accggcaata
     8401 tgctgtgggc caggcgtggt aatgaatttg tgacggtgaa atcgctgaac gagcagggcc
     8461 agccggtggg cgtggagata tttcattatc gcgacgatct ctcgcttgaa tcctacattt
     8521 atgcacgcag tgccaccatt gaagacgaca aaacgtgggt cctgcatggt gtgaatcata
     8581 aaaaatggct gaatggcaaa gaaacgctgg aaacattaga taatcttgcc tggcaatcgg
     8641 ccttcaccag tatgaatctt gaagagttat caatgccggg gaatactttt tctgtccgtc
     8701 agcttaatca ttacatccat tatttgcagg aaaccgggca acccagcagt gaataccgcc
     8761 ttgcactgtg ggaaaaactg gggcaaccca tcctgaccct ggcgatgatt ttgctggctg
     8821 tgccgtttac ctttagtgcc ccgcgttcgc ctgggatggg tagccgtctc gctgtaggcg
     8881 tcatcgtggg cttactcacc tggatcagct atcaaatcat ggttaatctg ggattgctat
     8941 ttgcgttaag cgcacctgtt accgcgctcg gtttaccggt agcgtttgtg ttggtggcgt
     9001 tgagcctggt gtattggtat gacagacaac attaaacccg ccgtggcgca aacaccacgg
     9061 cgggctcaca ttactgcttc agagtagcaa atgcctgaat gatggcatcg atttgctcac
     9121 gactgtgggc agcatttacg ctacagcgca gcagcgtgat gcctgcaggc gcggcaggcg
     9181 gcaggataag attcacatac acgcccagcg agataagctc gcgccagatg cgtaaacctt
     9241 cctctttcga accgattatt actggcacta ccgggctaat atgtgaactt aactcataac
     9301 ccagtttttg cagaccgtca tacagatggt tggcgttatc catcagcttt tgccgcagct
     9361 ctggttgtgt ggcaatagtt cttaacgacg agcgcacggt cgcaatgcag gacggagaag
     9421 gagaggcggt aaaaatatac gggcggctgc cgtagcgcag cacttcc
//
LOCUS       DBKEKI010000155         9364 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.156, whole genome shotgun sequence.
ACCESSION   DBKEKI010000155 DBKEKI010000000
VERSION     DBKEKI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.156"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(169..1545)
                     /gene="trkA"
                     /locus_tag="ACUQPX_003616"
     CDS             complement(169..1545)
                     /gene="trkA"
                     /locus_tag="ACUQPX_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HHV9058173.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1567..2856)
                     /gene="rsmB"
                     /locus_tag="ACUQPX_003617"
     CDS             complement(1567..2856)
                     /gene="rsmB"
                     /locus_tag="ACUQPX_003617"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HHV9058174.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2902..3849)
                     /gene="fmt"
                     /locus_tag="ACUQPX_003618"
     CDS             complement(2902..3849)
                     /gene="fmt"
                     /locus_tag="ACUQPX_003618"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HHV9058175.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3864..4373)
                     /gene="def"
                     /locus_tag="ACUQPX_003619"
     CDS             complement(3864..4373)
                     /gene="def"
                     /locus_tag="ACUQPX_003619"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HHV9058176.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4504..5628
                     /gene="dprA"
                     /locus_tag="ACUQPX_003620"
     CDS             4504..5628
                     /gene="dprA"
                     /locus_tag="ACUQPX_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HHV9058177.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5600..6073
                     /gene="smg"
                     /locus_tag="ACUQPX_003621"
     CDS             5600..6073
                     /gene="smg"
                     /locus_tag="ACUQPX_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HHV9058178.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6102..6644
                     /locus_tag="ACUQPX_003622"
     CDS             6102..6644
                     /locus_tag="ACUQPX_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HHV9058179.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6649..7221
                     /gene="tsaC"
                     /locus_tag="ACUQPX_003623"
     CDS             6649..7221
                     /gene="tsaC"
                     /locus_tag="ACUQPX_003623"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HHV9058180.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7226..8044
                     /gene="aroE"
                     /locus_tag="ACUQPX_003624"
     CDS             7226..8044
                     /gene="aroE"
                     /locus_tag="ACUQPX_003624"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HHV9058181.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEEVAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8041..8298
                     /locus_tag="ACUQPX_003625"
     CDS             8041..8298
                     /locus_tag="ACUQPX_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HHV9058182.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8274..8828)
                     /locus_tag="ACUQPX_003626"
     CDS             complement(8274..8828)
                     /locus_tag="ACUQPX_003626"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HHV9058183.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            9301..>9364
                     /locus_tag="ACUQPX_003627"
     rRNA            9301..>9364
                     /locus_tag="ACUQPX_003627"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2371 a         2267 c         2321 g         2405 t
ORIGIN      
        1 gcgcattgca aattcgcgaa attctttaat aatgctcatg ttattctccc tatgaagccg
       61 acaaccataa gtctaacaaa tgttaagcca ttttcctgcc aggaatgaat ttaattaatc
      121 cttgataatc aaagggaaat aataaggcgt cattagacgc cttattaatt acaagaagaa
      181 agggcttggc tggaagagtc tttcgacgtc ggtaataaac tttttatctg tgaggaacat
      241 aattacgtga tcgccttgct caatgcgcag attgtcattg gcaatcatca cgtcgtttcc
      301 acgtaccacc gctccaataa tcgttcctgg cggtagcttg atttcgtcaa tgactctgcc
      361 gacaacgcgt gaggtgcttt catcaccgtg agcaacggct tcaatagctt ctgctacgcc
      421 gcggcgcaat gaggaaacac caacaatatc tgcttttcgc acatggctaa gcaacgcaga
      481 aatagttgct tgttgtggtg aaattgcaat atcgatcacg ctcccctgaa ccagatccac
      541 ataagcgcga cgctggatca ataccatcac ctttttcgcc cccatacgtt tggcaagcat
      601 ggcggacatg atattggcct cgtcatcgtt ggtgacggca ataaacagat caacttgatc
      661 gatatgttct tcggccagca gttcttgatc cgacgcatca ccaaaaaaga cgatcgtatt
      721 ctgcaacttt tccgccagtt cggcagcgcg ctgctgatta cgttcgatga gtttaacgct
      781 gtaatctttt tccagacgac gcgccagccc tgcaccgata ttaccgccac caaccagcat
      841 gatccgctta tacggttttt ccagtcgctg taattcactc atcaccgcgc ggatatgctg
      901 tgaagcggca ataaagaaca cttcatcacc agcttcaaca atggtcgaac cttgtggacg
      961 aatggggcga tcgtggcgga aaattgctgc cacacgagta tcgatatgtg gcatatgttc
     1021 gcgcatggtc gaaagtgcat taccaatcag cgggccgcca taataggctt taactacagc
     1081 gaggctgact ttaccctcag cgaagttcac cacctgcaat gcgcctggat actcaatcag
     1141 tcggtaaata ttatcgataa ccaactgctc tggtgcgatc agatgatcaa tcggtacagc
     1201 atctgaatga aatagcttat cggcatcgcg tacgtagtct ggtgagcgga tgcgagcgat
     1261 gcgattaggg gtgttgaaaa gtgagtaggc tacctggcag gcaaccatat tggtttcatc
     1321 tgaactggtt acagcaacca gcatatcggc gtcgtcggca cctgcctccc gcaatacgcg
     1381 tggatgagag ccatgcccct gcacgactcg caggtcaaat ttatcctgca aggtccgcag
     1441 acgctcaccg ttggtatcga caacagtaat atcgttgttc tcgccaacca ggttttccgc
     1501 cagtgtgccg ccaacctggc cggcacccag aatgataatt ttcatcagtc gcgacccgtt
     1561 atctcatcac tttttgatta gcttagcgta aaagaagccg tcgccctctt cggcaccagg
     1621 ctgattttgt ttacccggtt gctctggtgt tcctgtttcg caaagttcgg catcagcggt
     1681 acgttgcaaa aaggctttaa tctgcaggct attctcttcc ggtaacaccg aacaggtggc
     1741 atagaccagg gttccaccgg attttaaatg cggccaaatg gcgtcgagaa tttcagactg
     1801 caattgcgcg agttccggga tatcgcgatc gcgacgtaac catttaatat ctggatggcg
     1861 acgaatcacc cccgttgctg aacaaggcgc atctaataaa atgcgatcaa actgttgctc
     1921 gccacaccat tgggaagggt aacggccatc accttgtttc acggttgcct tcataccaag
     1981 gcgttttaaa ttgtcgtaaa cgcgagagag gcgctgttcg tcaatatcaa ccgcaacaac
     2041 ctgcgcttct ggtgccacct caaggatatg cgttgtttta ccgccggggg cggcacaaag
     2101 atccaagatg tgttcaccgt tttgtggcgc aagccaggtc atgcaacctt gtgctgatgc
     2161 atcctgaacg gtaacccatc cgtcttcaaa accaggtagc gcatgaacag gtgcaggtgt
     2221 ttccagccgt acagcatcag ggtaatccgc atgcgggaaa cctttcattc ctgcttcatc
     2281 cagcaatgca agccagctgt cgcgggaatg atgcgtacga ttaatacgca gccacattgg
     2341 cggacgctgg ttattggctt cgacgatgga ttgccactgc tctggatacg ctttttgcag
     2401 acgcttcagc aaccaggaag gatgcagata acgtgcatca cgggtattaa actcggctaa
     2461 taactcttct tgccgacgct ggaactggcg taatacaccg ttaatcaacc ctttaagttg
     2521 cggacgctta attgcgatag cgccttcaac cgtttcagcc agcgcagcat gaggtggaat
     2581 gcgggtataa agcagttgat acaaaccaac cataatcagg taatgcacag tgcgctgttt
     2641 gccggtcatc ggacgggcca ttaacttatt aatcagccag tctaactgcg aaagcgtacg
     2701 cagtacgcca aagcacaact cttgaagaag tgctttgtct ttatcggaaa ctttttgctg
     2761 gagcggtggc agaatgttgc ttaatgattg cccttgctcg acgacttgtt caacggcctg
     2821 ggccgccatg ctacgtaaat tacgttgttt tttcataagt ataaaaatgc tcggcaagac
     2881 cgggcttaga agagtggact atcagaccag acggttgccc ggaacaaacc attcccgacg
     2941 agagttcagg aggtcttgcg cgctcatcgc tttcttaccc gcaggttgta acgagagcag
     3001 gttcaggatg ccatcaccag tcgcaacctg aatgccttgt ttgttggctt caaggatcgt
     3061 tcctggtgca gcgttggttg ccgtatcaat gaccgacgct ttccagactt taaccggctg
     3121 tccttcaatt tccagccagc tcattggcca tgggttgaaa gcgcgaatgc agcgttcaag
     3181 ctgtgctgcc gaaagtgacc agtcaatacg cgcttcttct ttactcaact tctcggcgta
     3241 agtgacaaga gtttcgtcct gaacttctgg tttcgccgtg ccgtctgcca attgtttcaa
     3301 cgtggtgata agcccttgtg ggccaagctc tgccagcttg tcgtacagcg taccactggt
     3361 atcttctgca gtaatcgggc aggagagctt atagagcatg tcaccggtgt ctaaaccaac
     3421 atccatttgc ataatggtta caccagtttc tgcatcaccc gcccatagtg agcgttggat
     3481 tggtgcagca ccgcgccagc gtggcagcag tgaaccatga acgttgatac agccaagacg
     3541 cggcatctcc agcacagctt taggcagaat taaaccatag gcgacgacga ccataacatc
     3601 agcctgcaaa tcggcgacca gttgctggtt ttcttgtgga cgcagggaaa caggttgaaa
     3661 aacgggcaga ccttcttcct cagccagaac tttaatcggg ctgggcatca gttttttacc
     3721 gcgtcctgcc ggtcggtctg gctgggtgaa cacgccaacg acgttatgac cagaagacaa
     3781 cagcgcgtcg agatgacgcg ctgcaaagtc aggtgtaccc gcaaaaataa tacgtagtga
     3841 ttctgacacg ttagttctta tccttaagcc cgggctttca gacgatccag tttttcaact
     3901 ttctgacgaa ttcgttgttg tttcagcggt gacagataat ccataaacag tttgccaacc
     3961 aggtgatcca tctcatgctg aatacagata gctaacagac cctctgcttc cagttcaaat
     4021 ggtttaccgt cgcggtcaag ggcgcgaatt ttaactttct ctgcgcgcgg cactaaagca
     4081 cgttgttcag ggatcgacag gcaaccttct tcaatgcctg tttcgccgct tttttctaaa
     4141 agctccggat tgattaacac cagccgttcg tcacggtttt ccgaaacatc aataacaatg
     4201 atacgttgat ggatatcaac ctgggttgcc gccaggccaa taccttcttc tgcgtacatc
     4261 gtctcgaaca tatcatcgac gatacgctga atttctgcat tcacttcttc taccggttta
     4321 gcaactttgc gaagccgctc gtccggaata tgtaacactt gcaaaactga cataaatctc
     4381 cagagatgtg ttcaggagtt agaaagatta tttcttctat tctagacaaa tccccctctg
     4441 attgacagca tcactgacca atcgcaaaga ttgctaaggc ttgcttatgg cagggagata
     4501 aggatggtcg atacagaaat ttggctgcgt ttaatgagca tcagcagctt gtacggcgat
     4561 gatatggtcc gtatcgctca ctggctggca aaacagtcgc atattgatgc ggttgtattg
     4621 cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc acgaaagagt
     4681 atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc tgcggacagc
     4741 gaattttatc ctcctcaact tcaggcgacg acagattacc ccggcgcact gtttgttgaa
     4801 ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg ggcgcattca
     4861 tggtatggcg agcgatgggg gcgattattt tgcgaaactc tggcgacgtg tggagtgaca
     4921 attacgagtg gactggcgcg tggaatcgat ggtgtggtgc ataaagcggc cttacaggta
     4981 aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc gcgtcgccat
     5041 gcccgactgg ctgccagtct gtttgaacag ggtggcgctc tcgtctcgga atttcccctc
     5101 gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag tggtctaagt
     5161 aaaggtgtac tggtggtgga agcggctttg cgcagtggtt cgctggtgac agcacgttgt
     5221 gcgcttgagc aggggcgtga agtttttgcc ttgccaggtc caatagggaa tccgggaagc
     5281 gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc ggaagaaatt
     5341 ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa ttcattttat
     5401 tcaccagatc agcaagacgt ggcattgcca tttcctgagc tcctggctaa cgtaggagat
     5461 gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc agaggtagtt
     5521 actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg cggctatgtc
     5581 cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa cctatattca
     5641 cactgaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta ccgacgcagg
     5701 ttttgagcga gaagatatct acaatgccct gctatggctg gaaaaacttg ctgattatca
     5761 ggaagggttg gcagaaccga tgcaactggc ctcggatcct ctctccatgc gtatttatac
     5821 accggaagag tgtgaacgac tggatgccag ctgccgtggg tttctgcttt tccttgagca
     5881 gattcaggtg ctcaaccttg aaactcgtga aatggtgata gagcgagtgc tcgcgctgga
     5941 taacgcagag ttcgaactgg atgatctgaa atgggtgatc ctgatggtgt tgttcaacat
     6001 cccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag tgaatgaagg
     6061 tatgctgcat taatcatttc tttaattcag cataagttgt tatggcaaaa tcagcactgt
     6121 tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt attcgatccg
     6181 gaaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac gtccgtcctc
     6241 tgaaatcttc agcggatgga catattgtca aagttctgga ggggcaggtt tgccccgtat
     6301 gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt tgcagtaact
     6361 accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca attacatgcc
     6421 cccaatgtcg gacgggccat ctggtccagc gccgctcccg ttatggcaaa acatttcact
     6481 cctgtgatcg ctacccggag tgtcaatttg ccattaactt caaaccaata gctggagaat
     6541 gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt gtaaaacact
     6601 tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt gaataataac
     6661 ctgcaaggag acgctatcgc agctgcgata gatgtcctca atgaaaaacg tgtcatcgcc
     6721 tatccaacgg aagccgtttt cggtgtcggg tgcgatcctg atagcgaaac agcagtgatg
     6781 agactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat cgcggcaaat
     6841 tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgtgca gcgtgaaacc
     6901 attttttcct gctggccagg tcctgtcacc tttgtctttc ccgcgcctgc gacaacaccg
     6961 cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca tccgttggtg
     7021 gttgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa cttgagtgga
     7081 ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc gttcccggtt
     7141 gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc cctgacgggt
     7201 gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta atccgatagc
     7261 ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata ttgaacatcc
     7321 ctatgggcgc gtgttggcac ccatcaatga tttcatcaac acactgaacg ctttctttcg
     7381 tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt ttgccagagc
     7441 ggatgagctt actgaacggg cagcgttggc tggtgcagtt aataccctca agcggttaga
     7501 agatggacgc ctgctgggtg acaataccga tggtgtaggc ttgttaagcg atctggaacg
     7561 tctgtctttt atccgccctg gtttacgtat tctgcttatc ggcgctggtg gagcatctcg
     7621 cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacta atcggacggt
     7681 atcccgcgcg gaagaggtgg ctaaattgtt tgcgcacact ggcagtattc aggcgttggg
     7741 tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgcaacat ccagtggcat
     7801 cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt attgctatga
     7861 catgttctat cagaaaggaa aaactccttt tctggcatgg tgtgagcagc gaggctcaaa
     7921 gcgtaatgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct ttcttctctg
     7981 gcacggtgtt ctgcctgacg tagaaccagt tataaagcaa ttgcaggagg aattgtccgc
     8041 gtgaatcagg ccatccagtt tccggacagg gaagagtggg acgagaataa aaaatgtgta
     8101 tgttttcccg ctctcgtgaa tggtatgcaa ctgacatgcg cgatctctgg cgagagtctg
     8161 gcgtatcgct ttactggaga tacgccagaa cagtggttag cgagttttcg tcagcatcgc
     8221 tgggacctgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga tgatcaaggc
     8281 tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt attcgcggaa
     8341 tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac ggggctaccg
     8401 agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc cgcaccaatc
     8461 atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc aaccaaaact
     8521 cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac atcttcgcca
     8581 atggttaatg ggttgccatc tgggttgtac gaggatttat gagtgacatg caacatactg
     8641 ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc acgaatcaca
     8701 acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac gacactgcta
     8761 tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg gcgtaaaaca
     8821 tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc attattggcc
     8881 ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgattgc agcaaaaagg
     8941 gtgaaaaaac aacaaacaga aaaaaagatc aaaaaaatac ttgtgcaaaa tattgggatc
     9001 cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaggatg gtcggaacaa
     9061 cgaagagaaa aaatcctgaa attcagggtt gactctgaaa gaggaaagcg taatatacgc
     9121 cacctcgcga cagtgagctg aaagccgcgt cgcaactgct ctttaacaat ttatcagaca
     9181 atctgtgtgg gcactcgaag atacggattc ttaacgtcgc aagacgaaaa atgaatacca
     9241 agtctcaaga gtgaacacgt aattcattac gaagtttaat tctttgagca tcaaactttt
     9301 aaattgaaga gtttgatcat ggctcagatt gaacgctggc ggcaggccta acacatgcaa
     9361 gtcg
//
LOCUS       DBKEKI010000156         9004 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.157, whole genome shotgun sequence.
ACCESSION   DBKEKI010000156 DBKEKI010000000
VERSION     DBKEKI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.157"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..364)
                     /locus_tag="ACUQPX_003628"
     CDS             complement(<1..364)
                     /locus_tag="ACUQPX_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HHV9058184.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARR"
     gene            complement(561..1361)
                     /gene="ydcF"
                     /locus_tag="ACUQPX_003629"
     CDS             complement(561..1361)
                     /gene="ydcF"
                     /locus_tag="ACUQPX_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HHV9058185.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIAHDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTVRTTGRAEATIL
                     VDIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(1633..5535)
                     /gene="hrpA"
                     /locus_tag="ACUQPX_003630"
     CDS             complement(1633..5535)
                     /gene="hrpA"
                     /locus_tag="ACUQPX_003630"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HHV9058186.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5736..6341
                     /gene="azoR"
                     /locus_tag="ACUQPX_003631"
     CDS             5736..6341
                     /gene="azoR"
                     /locus_tag="ACUQPX_003631"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HHV9058187.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(6395..7705)
                     /locus_tag="ACUQPX_003632"
     CDS             complement(6395..7705)
                     /locus_tag="ACUQPX_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HHV9058188.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWSHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALPYVVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKL
                     PPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAVLDVTFEF
                     PRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALV
                     VAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(7695..>9004)
                     /locus_tag="ACUQPX_003633"
     CDS             complement(7695..>9004)
                     /locus_tag="ACUQPX_003633"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HHV9058189.1"
                     /translation="ARPALALWHRLRGLFFINSYVKGRYLTHDRQRVASFNNDPLITR
                     AIAVNILLDLYKTSERIVSDAAAITLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKEL
                     HLLPGFYHDTLGEENRAQAFEKMQSFICRLYANKSQKFDYQHEDRTGPSADRWRLLSG
                     GPVPLSPVDLAYRFMRKAMKLFGAHSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFG
                     RLIDKIYLNSVGWRGIRQRKTHLQILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDAL
                     ANEPAVSDILLRDYSELNVAQGQEMIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLA
                     IVSGLYELFPENEQVKNSLAGLANAIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKP
                     WVMRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT         2211 a         2428 c         2182 g         2183 t
ORIGIN      
        1 aacgccgtgc ccactccgcc atgtaatcga tatagtcggc agtaaaagcc acttcgactt
       61 cagccagctg ctggatcttg cccccttctt caacaatcag cgcactgatt tcactggcgc
      121 gttcgcggat cccggcggag attttgcgca accaactggc gcgttcaata gcaggcaacg
      181 cttcccattc tggttgtgca cgttctgctg catcgattgc cttacgggca tcctcggcct
      241 gaccatcggg tatgcgggaa atgacagcct ctgtagcagg gtttaccaca tcaatccatg
      301 cgtctccacg ccaggtaaca aactgtccat cgatatacat aggatgttga acgggtactg
      361 acatgggcga ctcctgtgat ttatatgttt gttttcggtg aatatattgt taattaacat
      421 caatcatcag agagacggca tcgggaaagg acaattctgt ggagggcttc ataaaaacaa
      481 gtgtaaaagg aataaaaggc agaaataaaa gtcaggagtc atgcccgttt gcaaacaagt
      541 ggttcacaaa cgggcaattt ttaacgtaac gagcgacttt ccatcgcctc gatgagcacc
      601 gcatcatgtt tcagcgtttg ccatgcatgg atgacttctg ccggaaaatc aacgtgaacg
      661 ataaaatctc gcccgcgggg accgtagcca tcgctatcat cgtgtaaacg cggcagctcg
      721 ccagtgagta gtgagagata acgctcaacc ggccataatc cttgtaactg attaataaag
      781 attacactgt ctgcgttatt tcctaactga ggaacgaatc cgggataact taaccagcgt
      841 ggtgcatcgg gattgtcccc ggtcatacgg cggaacgttg ccatcgtgcg ccgctgcatg
      901 gtggggtcct gtacaacgat agccgtatga actcgttcta cggcctgatt caatagcgcg
      961 atgctaaagc gtgcgttttc accgcagttt gttgactggt cttcaatcca gattttttca
     1021 tgcggaatat gccagaactg atgagcgata tccaccagga tggtcgcttc tgctctgcca
     1081 gtggtgcgga cagtgttgta gtgcggatgc tgtgcgatgg cgctatacaa aaaagtggtc
     1141 gagtgaccga taccaccact aatcagtaaa ggaatttgct gatcgtgagc aatcttacat
     1201 gccgcatcga tagtcggcat aaccgcattt cctgcaagta tgacgcaatc agcctgatac
     1261 ggcacctcac cggagaaatc atcctgcgcc agccactgtc cgataacatt tatggcatct
     1321 atagttgccg tcgaaagcgt cggaaacggg gtaatgttca tggttcctcc ttatcgttcc
     1381 cttaagaata ctgcgtgaga taatagtgag aagcggagca ctctgaaagg aggaagtcgt
     1441 atttccttaa ccgccactcc gcaagcctga catatctttt aaggaaatgc tgtagataaa
     1501 cattattcat cttgcagctg agaattgtat ttttctgaat tcctgaattt ttgtccttac
     1561 cgttttagaa ttaataaaaa acccggcgct aaatgcttac cgggttttag atttattagg
     1621 caaatagcag ggttaaccgc taatctgctc catcgcctgc aaaatacgct tatctgaaat
     1681 cggataaggc gtaccaagtt gttgagcgaa gtaactaacg cgcagctctt ctatcatcca
     1741 acggatctct ttcacgtctt catcctcacg acgtgcaggc ggcagtttgt tgatccattg
     1801 ctgccacgcc tgctggacgt tttcgacttt cagcatctgt gcacgatcgc gatgtggatc
     1861 aaccgccagt ttttccagtc gtttttcaat cgcctgcaaa tatcgcagcg tgtcgcccag
     1921 ccgtttgaag ccgttaccag tgacaaaacc gcgatatacc aacccgccca tctgcgcttt
     1981 aatgtcagaa agccccagtg ccatggtcat atccacccgc cctttcagac gtttgttgat
     2041 attgaacacc gcagtgagga tttgctcgac ctgcttcgca atatccacca ccgtgtcatt
     2101 cagttcggcg cgcacttttt catgcagcgc agcaaagcct tcttccgtcc agaccgggcc
     2161 accattggcg tcgatcagtt gatccacacc gcaggagata cagtcgtcga tcagctccag
     2221 cactttgcca tacgggttaa agtacagccc cagcttggct ttgtttggta acttctcatg
     2281 taaatacttg atcggcgagg aaatatttag cagcagtaga cggcgaagac cattccacat
     2341 tgcctgtttt tgctccagcg ggttatcaaa cagtttgatc gccacgctgt cgcgttcatc
     2401 caccagcgcc ggccacgcct tcactttata gttgccacgc ttctgttcgt aactttccgg
     2461 cagctgacca aaactccaga tatgtaagcc gctctgctcg ataccgtcat ccgccaccgc
     2521 ggacagcgtt tcctgcactt tgcctttcag cgcatctttc agatcttgta gcgagcgccc
     2581 ttcttttagc ttcttgtttt tgtcatccac cacgcggaag gtgattttca ggtgatcggg
     2641 cacctgatcc cagtgccagt cttcgcggtc aacggtaacg ccggtcatcc gccgtaactc
     2701 gcgctcaagg ctgtcgagca acggtaactc cagcggtgtg acgcggccta aaaacgcttc
     2761 ggcgtagttt ggtgcaggca caaaattacg gcgtaccggt ttcggcaacg atttaatcag
     2821 agcaatcacc aattcgcggc gcagaccggg gatctgccat tcaaacccgc tttcctcaac
     2881 ctggttaagt aacggcagcg gaatatgtac ggtcacaccg tccgcgtccg cgcctggctc
     2941 aaactgatag ctcaaacgca gcttgagatt gccctgatgc cagaagttcg ggtaatccag
     3001 cttgctgatt ttttccgccc cttctttgat caacatgctc ttttcaaagt tgagcaaatc
     3061 aggcgtttcg cggctgactt ttttccacca gctatcgaag tgacgagcgg agattacatc
     3121 gtggctgatg cgctggtcgt agaactcaaa cagcgtttcg tcatcaacca gaatatcgcg
     3181 gcgacgtgat ttgtgttcca gctcttccac ttcggcccgc agtttcaggt tttcacggaa
     3241 gaatgcgtga cgcgtctgcc agtcaccttc caccagagcg tggcgaataa agagttcacg
     3301 acataacgcc gaatcgatct ggctgtagtt gaccttgcgc gcagcaacaa tcggcaaacc
     3361 ataaacagtg actttttccg ttgccatcac cgcgccctgc gcccgttccc agtgcggttc
     3421 gctgtaggtg cgtttaatca aatgctgagc aacgggttct acccattccg ggtcgatacg
     3481 cgcagcaatg cgcccccaca ggcggctggt ttctaccagt tccgccacca ttacccattt
     3541 cggcggcttt ttgaataaac cagaaccggg gaagatggag aaacgcgcgt tacgtgcacc
     3601 ggtatattcc tgtttatcgg catctttcat gccgatatgg gaaagcaaac cggtcagtaa
     3661 ggcgatgtga atttcacgat actccgccgg ttcgctgttg accggaatgc caagttcttt
     3721 caccacctgg cgcaactggg tgtagatatc ctgccattcg cgcacgcgca gatagttgag
     3781 gtaatcggta cgacacaggc gacggaaggc gttggaagaa agcgcctttt gctgctcgcc
     3841 aagataattc cacagattta caaacgcgag gaagtcggat tctttgtcgt ggaagcgacg
     3901 atgtttttcg tccgatgcct gctgtttgtc catcggacgt tcgcgcggat cctgaatgga
     3961 gagcgcggac gtgataatca tcgcctcacg cacgcagcca tgtttttgcg cttccagcac
     4021 catacgcgcc agacgtgggt cgacaggcaa ctgcgagagc tggcgaccga gcggcgtcag
     4081 tttataggcg ctggcctgtt catccgtggt gatcgcgccc agttcttcga gcagacgcac
     4141 gccatcctgg atattgcgtt tatccggtgc ttcgacaaac gggaacgcag cgatatcgcc
     4201 cagccccagc gcggtcatct gcaaaataac cgaggccagg ttggtacgca gaatctccgg
     4261 atcggtaaac tccgggcgcg agaggaaatc gtcttcggaa tagagacgaa tacagatccc
     4321 ttcggacaca cgaccacagc ggcctttacg ctgattggca gacgcctgcg aaatgggttc
     4381 aatcggcaaa cgctgcactt tggtgcgata gctgtagcgg ctgatacgcg ctgtaccggg
     4441 gtcgatgacg tatttaatcc ccggtacggt cagcgaggtt tccgcgacgt tggtcgccag
     4501 cacaatgcgc cgtccgctgt gcgactggaa cacgcggttc tgttcgctgt tcgaaagccg
     4561 cgcataaagc ggcaagattt cggtatggcg taagttcagc ttgttcagcg catcggcggt
     4621 atcgcgaatt tcccgctcgc cgctcataaa gatcagaatg tcgccggggc tttcctggct
     4681 tagttcgtct acggcgtcaa aaatcgcctg caactgatcg cgctcggtgt catcggcttc
     4741 ttcaacaatc gggcgatagc gcacttccac cggataggtc cgaccggaga cttcaataat
     4801 cggcgcatta ttaaagtggc gcgaaaagcg ttccgggtcg atagtcgcgg aagtgataat
     4861 gatttttagg tcaggacgcc gcggcagcaa ctctttcaaa tagccgagca gaaaatcgat
     4921 attcaggctg cgttcgtgcg cttcgtcaat aatgatggtg tcgtactgca tcagcaggcg
     4981 gtcttgctgg atctccgcca gcaggatccc gtcggtcatc agcttgacca tcgtgttatc
     5041 acttacgtga tcgctgaaac gcactttgta accgatgcaa ccgcccggct ccgttttcag
     5101 ctcttccgca atacggttgg ccaccgttcg tgccgccagg cgacgcggct gggtatgacc
     5161 gatcagccct ttaataccgc gccccagctc catacagatt ttcggtagct gagtcgtttt
     5221 accggagccg gtttccccgg cgacgatcac cacctggtga tcacgaatcg cttcgagtat
     5281 gtcctgtttt ttctgactaa ccggtaaatt gtcaggataa gtgatttccg gtcgtgccgc
     5341 ttcacgcagc aggactttcc ctgccgcctg gtcaatctct ttcgccatct cctggaaaat
     5401 ggcctgttgt gcatcaggat ttttaacctt cttaacacaa tgcagacggc gagaaaaacg
     5461 cagtctgtcg cgcagcatca gcgaatccag ccgctgttgc aaggcagtaa aggtcaattt
     5521 ttgttgttct gtcataacgt tagtgggcag tagtctgcct gattaatttt cttttaaatg
     5581 atagatatag agtaccacat cgcggctttc tatgcgttgt tcaataaatt cgaacataga
     5641 cttcgattta ttgcgctatc tctgtggcag gattgtgaat aaagtgtcag caagcaacgg
     5701 ggcatcgtcc attcaaacat ctataaggaa acaccatgag caaggtatta gttcttaaat
     5761 ccagcatcct ggcagggtac tctcagtcta atcagttgtc cgattatttt gttgaacaat
     5821 ggcgcgaaaa gcactccgct gatgaaatca ccgttcgcga cctagctgca aatccgattc
     5881 cggtactgga tggcgaactg gttggcgctc tgcgtccgag cgatgcgccg ctgactccgc
     5941 gtcagcagga agctctggcg ctttccgatg agctgattgc cgagctgaaa gcccacgacg
     6001 ttatcgttat tgcggcaccg atgtataact tcaacatctc aactcagttg aaaaactatt
     6061 ttgacctggt tgcacgcgca ggcgttactt tccgctatac cgagaacggt ccggaaggtc
     6121 tggtaacggg taaaaaagcc atcgttatta ccagccgcgg tgggatccac aaagatggac
     6181 caacggacct ggtgacgccg tatctgtcca cgttcctcgg ctttatcggc attaccgatg
     6241 tgaaatttgt cttcgccgaa gggatcgcat acggtccgga aatggcagcg aaagcacagt
     6301 ctgacgcaaa agcagccatc gacagcattg ttgctgcata accggacacg gtcttgatgt
     6361 ggactgtaga tattcagtcc acatctcaat ccacttacct gttttcccat aatttcaaca
     6421 tcgctttgtg ctcttcctta agcacaatat gcgagcgtct ggctcgaata aagctaatcg
     6481 cttcatcaac tgttttacag tgtccgtaac ataacagcca tgccgccact accagcgcac
     6541 tgcgcgataa tcccaatgcg caatggacca gaacgctgcc ttgctcttcg cgtaatgttt
     6601 ccagcatcgc cacggcctgt cggagctccc cctcttccgg aaccaccaga tccaacatcg
     6661 gtacacagaa atagagtcga tcttttgtcg ctcgtccacg agggaattca aaggtgacgt
     6721 ccagaaccgc attctgtgcc ggaatatgtc gtggaaacgc ccttaaataa acaccagcag
     6781 tcattttgct caccggctcc aggcgacgac aaaaccaacg catagatagc cacatcccga
     6841 tgcgccaggg caatgtcagc cagtaaacgg cgggcgggag tttcccctga ctatctttcc
     6901 ctgttgttat cgcgccaagc ccaccgtacc cacggccaat aatgagtagc gataatactg
     6961 gccaacataa ccagactgac caccataact gaaccatcat cattagctcc atcaacacaa
     7021 tgcacgcgcc tacgacatat ggcagtgcta ttttgattcg acgttgatca agtttctgat
     7081 aattccaacg acggtcgacg ggcaccatcc agtcaatcaa catacctacc gccagccctg
     7141 tgatgacatc aataaaatga tgctgccatg tcgtcagcgt cgaaatggcg atgagtaaaa
     7201 accatccgcc acagacttta cgccacctca cagccagatg ctgacgaaag tgactccaaa
     7261 gtagccagca gagaataata tgcagcgaag gagactggtt ataaggcaga tcaaacagtt
     7321 caagttgcga aaatagccat cctgtcaccc cactcacttc aggacggata aaactaaact
     7381 tcagcgggta gagaaaaaaa ccgcagcagg ccattaccgt tgccagaata agccggtgga
     7441 caagtcggcg ttgttcgaat gtcgagctac aaacgaacag cgaaaatcca tataaaagat
     7501 ccagactcca gtaaggaaca atagtccagg gaagaaaagg gatcgccgtt tcccaaccga
     7561 atacctgact ggggatatca tggctgttaa ggtcctgaac cgcggtgaac tgattaagag
     7621 atccataggt gaagaagaaa aacggggcca gcaacaataa ccagccagcg ccttgtagca
     7681 atacgttccg acgttcagtt atcacgacgc accgccatcg aaaccgtaaa aatgccccac
     7741 acatcaatcc gttgagtgca tttatcaaat ccggcattac gcacgagcga atccatctcc
     7801 ccttgcgaac gcacgcgcat tacccacggt ttaccatctt tatgactggt taacaccccg
     7861 gcaatcagct ccagttgtgg gtgccacggc tgcccggtgt agatgagaat gccacccggt
     7921 tcgatggcat tggctagacc tgcgagtgag ttttttacct gctcattttc gggaaaaagc
     7981 tcatataggc cagagacaat cgccagcgta ggccgcggag ttaacgcgct gagttcctcc
     8041 gggttaaacg catcgccctg ttcaaaacgc acccgcccag acattccccg ttgggcaatc
     8101 atctcttgcc cctgtgcaac atttaactca ctgtaatcac gtaacaaaat atcgcttacg
     8161 gcaggctcgt ttgccagcgc atccagtaca tagcgtccat gccctgcggc aatgtcaacc
     8221 acgcggacgg ctaaaccttt ggcgtggaga tcggcaacgg cttgtttaat caatatttgc
     8281 aaatgggttt tgcgctggcg aataccgcgc catccaacac tgttcaggta gattttgtcg
     8341 attaaacgcc cgaatgcgtt actgccttgc ggttgatttt gatagacata atccagggaa
     8401 ctgcctgaat caaagccggt actcattccg agatgcaggc ccgcagagtg cgcaccgaac
     8461 aatttcatcg cctttcgcat aaagcgatac gctaaatcaa ccggcgataa tggcacgggt
     8521 ccaccagaaa ggagccgcca tcgatccgct gatggtcctg tgcggtcttc atgctgataa
     8581 tcaaattttt gcgatttgtt agcatataag cggcagataa agctttgcat tttttcaaat
     8641 gcctgcgccc tgttctcttc acccaatgtg tcgtgataaa agcctggcag cagatgcagc
     8701 tctttcagag ggctacgtaa tctctgataa aaatcaatct gcggttggcg atgcaccaca
     8761 tagtcatcac ctgatatcag aagttgcgtg gggagcgtaa tcgccgccgc atcgctaaca
     8821 atacgttcag acgttttata gagatcgagc aagatattga cggcaatcgc ccgtgtgatc
     8881 agcggatcat tattgaaact cgccacccgt tgccgatcgt gggtcaaata gcgccctttc
     8941 acataggaat taataaaaaa tagaccacgc agacgatgcc ataacgccag cgcaggacgt
     9001 gcca
//
LOCUS       DBKEKI010000157         8903 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.158, whole genome shotgun sequence.
ACCESSION   DBKEKI010000157 DBKEKI010000000
VERSION     DBKEKI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.158"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..2894
                     /locus_tag="ACUQPX_003634"
     CDS             <1..2894
                     /locus_tag="ACUQPX_003634"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HHV9058190.1"
                     /translation="VVLLTPGDDPLRAFLIWVLSESIGALALVPLGLLFKPHYLLRHR
                     NPRLLFESLLTLAITLTLSWLSMLYLPWPFTFIIVLLMWSAVRLPRMEAFLIFLTTVM
                     MVSLMMAADPSLLATPRTYLMSHMPWLPFLLILLPANIMTMVMYAFRAERKHISESET
                     RFRNAMEYSAIGMALVGTEGQWLQSNKALCQFLGYSQEELRGLTFQQLTWPEDLNKDL
                     QQVEKLISGEINTYSMEKRYYNRNGDVVWALLAVSLVRHTDGTPLYFIAQIEDINELK
                     RTEQVNQQLMERITLANEAGGIGIWEWELKPNIFSWDKRMFELYEIPPHIKPNWQVWY
                     ECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIAVKDGIRHIRALANRVLNKEGEVERLL
                     GINMDMTEVKQLNEALFQEKERLHITLDSIGEAVVCIDMAMKITFMNPVAEKMSGWTQ
                     EEALGVPLLTVLHITFGDNGPLMENIYSADTSRSAIEQDVVLHCRNGGSYDVHYSITP
                     LSTLDGSNIGSVLVIQDVTESRKMLRQLSYSASHDALTHLANRASFEKQLRILLQTVN
                     STHQRHALVFIDLDRFKAVNDSAGHAAGDALLRELASLMLSMLRSSDVLARLGGDEFG
                     LLLPDCNIESARFIATRIISAVNDYHFIWEGRVHRVGASAGITLIDDNNHQAAEVMSQ
                     ADIACYASKNGGRGRVTVYEPQQAAAHSERAAMSLDEQWRMIKENQLMMIAHGVASPR
                     IPEARNLWLISLKLWSCEGEIIDEQKFRRSFSDPALSHALDRRVFHEFFQQAAKAVAS
                     KGISIALPLSVAGLSSATLVNDLLEQLENSPLPPRLLHLIIPAEAILDHAESVQKLRL
                     AGCRIVLSQVGRDLQIFNSLKANMADYLLLDGELCANVQGNLMDEMLITIIQGHAQRL
                     GMKTITGPVVLPLVMDTLSGIGVDLIYGDVIADAQPLDLLVNSSYFAIN"
     gene            complement(3004..3852)
                     /gene="alkA"
                     /locus_tag="ACUQPX_003635"
     CDS             complement(3004..3852)
                     /gene="alkA"
                     /locus_tag="ACUQPX_003635"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HHV9058191.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3986..5338
                     /gene="yegD"
                     /locus_tag="ACUQPX_003636"
     CDS             3986..5338
                     /gene="yegD"
                     /locus_tag="ACUQPX_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HHV9058192.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5350..7290)
                     /gene="yegI"
                     /locus_tag="ACUQPX_003637"
     CDS             complement(5350..7290)
                     /gene="yegI"
                     /locus_tag="ACUQPX_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HHV9058193.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKLALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALWSFGIETAAGVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(7287..8048)
                     /locus_tag="ACUQPX_003638"
     CDS             complement(7287..8048)
                     /locus_tag="ACUQPX_003638"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HHV9058194.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8045..8704)
                     /locus_tag="ACUQPX_003639"
     CDS             complement(8045..8704)
                     /locus_tag="ACUQPX_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HHV9058195.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRTINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2167 a         2354 c         2328 g         2054 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gtgattcgtt gcaatcacgc tcgcccttta
     1081 agctggaatt tcgcattgcc gtgaaagatg gcatccgcca tatccgcgcc ctcgccaacc
     1141 gggtactgaa taaagaaggc gaagtcgaac gcctgctcgg cattaatatg gatatgaccg
     1201 aggttaaaca gcttaacgag gcattgtttc aggaaaaaga gcgcctgcac attacgctgg
     1261 attccatcgg tgaagccgtg gtctgtattg atatggcgat gaaaattacc tttatgaatc
     1321 cagtggcgga gaagatgagc ggctggacgc aggaagaagc attaggtgtt ccgctcctga
     1381 cggtgttgca tattactttt ggcgacaacg gaccattaat ggagaacatt tacagtgccg
     1441 acacctcacg ttccgcgatt gaacaagatg tggtgttgca ctgccggaac ggcggcagtt
     1501 acgacgtgca ttacagtatt acgccgttaa gtactctgga cggcagcaat ataggctcgg
     1561 tactggtgat tcaggacgtc accgaatcac gcaaaatgct gcgccagctg agctacagcg
     1621 cctcccatga tgcactgacg catctcgcca atcgcgccag ttttgagaag caactgcgta
     1681 tcctgctgca aacggtaaac agtacacatc agcgacatgc cctggtgttt atcgatcttg
     1741 atcgctttaa agcggtgaat gacagcgccg ggcatgcggc gggtgacgct ttactgcgcg
     1801 aactggcgtc gttgatgctg agtatgctgc gctccagcga cgtgctggcg cgactcggtg
     1861 gtgatgaatt tggtctactg ttgccagact gtaatatcga aagtgcgcgt tttatcgcta
     1921 cacgcattat cagtgccgtg aatgattatc actttatatg ggaaggccgt gtgcatcggg
     1981 taggtgccag tgccgggatt accttgattg atgacaacaa tcatcaggcg gcagaagtga
     2041 tgtcgcaggc tgatatcgcc tgttatgcct ccaaaaatgg tggacggggc cgggtgacgg
     2101 tttacgaacc acagcaagct gccgcacata gcgaacgggc ggcgatgtcg cttgatgaac
     2161 agtggcggat gattaaagag aatcagttga tgatgatcgc ccacggtgtc gcttcgccgc
     2221 ggatcccgga agcgcgtaat ttgtggctga tttcacttaa gctctggagt tgcgaaggcg
     2281 agattattga tgaacaaaaa tttcgtcgta gcttcagcga tccggcactt agccatgctc
     2341 ttgaccgacg ggtattccac gaatttttcc agcaggccgc aaaagcggtt gccagtaaag
     2401 gcataagcat cgccctcccc ctttccgttg ccggtttgag tagcgccacg ctggtgaatg
     2461 atctgcttga gcagctggaa aatagccctc taccaccacg gttattacat ctgattattc
     2521 cggctgaagc gattttagat cacgcagaaa gcgtgcaaaa actgcggctg gcgggatgtc
     2581 ggatagtgct cagccaggtg ggccgcgatc tgcaaatctt caactcgctg aaagcgaata
     2641 tggcagatta cctgctactt gatggtgagt tatgcgccaa cgtgcagggt aatttgatgg
     2701 atgagatgct gattacgatt attcaggggc acgctcagcg actcgggatg aaaaccatca
     2761 ccgggccagt cgttttaccc ttagtgatgg atacgctttc tggcatcggc gtcgatctga
     2821 tttatggtga tgtgattgcc gatgcccaac cgctggattt gctggtgaat agcagttatt
     2881 tcgcgattaa ctgagagagc gtttgcccat tgcctgatgc gatgctaacg cgtcttatca
     2941 ggcctacaaa tcgctcattc cgcaggcggg ataaggcgct cgcaccgctt ccggcgacca
     3001 acgtcatgct tcgtctggtt gccagccttc cgtataccag atatgcaaca gcgcataaga
     3061 acgccagggc ttccagcgct cggcataacg gcggatttgt gccggtgtca tccccagaaa
     3121 tcgctgttta atcagataat catccggcag aaaaacatct ttcgcctgcc agccgcgcaa
     3181 ggcgaaataa ttcgccgtcc agcgcccgat acccggaaaa gtttgcagcg ttttcatcgc
     3241 ctgctccaca tcgcccggta ttgtcattgg taaggtgccc tccagcgccg catttgccag
     3301 atgaatcagc gcctctgccc gtttcaacgg catacctaac gcttttaatg cctgcgggtc
     3361 ggctgctgcc agccgctgag gcgtggggaa gcagatatac tccggaaaat catccagccg
     3421 ttcgccataa agctgtgcca ctctggcggt caattttgcc gccatcgcca cgctcaccag
     3481 ttggcctaaa atcgcccgca cgccctgctc aaaagcatca acactgccgg gtaaacgcaa
     3541 tccgggccgc gccgcgccta acttgcccaa cgcaccgtta acaatctgcg ggttacattg
     3601 cagatcaagc aggcggctca ttttcgccag acactcagcg gcaacgggtt ctaaacctgc
     3661 acttaaattt atgtgcagag tatggcgggc tatatccgga atagcagtca ccacgccgcg
     3721 atattcgccc actgccagac tacgggcata ataactgtcc gcgaccgttt ccacaccact
     3781 caccgcacgg gcggcgagaa atcccaacat ccacgaccag tcatacggcg gctgccagtt
     3841 caggatatac atcgcatctc cttattcatc cgctttcagc ataaacgtta ttcagacgtt
     3901 acgctttgct ttcatattcc ggttgtcgcg acggcaacat ttcgctaaag tcacgcccct
     3961 tcttcaccgg catggggatt atttcgtgtt tattggtttt gattacggta cagcaaactg
     4021 ttcagtggcg gtcatgcgtg acggtaaacc gcatttgcta aaaatggaaa acgacagcac
     4081 gctgctgcct tcaatgcttt gcgcgccaac gcgtgaagcg gtaagcgaat ggctgtaccg
     4141 ccatcatgac gttccggcag acgacgatga aacgcaggcg ttgttacgac gggcgattcg
     4201 ttataaccgc gaagaagata tcgatgttac agcgaaaagc gtgcagtttg gtctttcctc
     4261 actggcacag tacattgatg atccagaaga agtgtggttt gtgaaatcac caaaatcgtt
     4321 cctcggtgcc agcggcttaa aaccgcagca ggtagcgctg tttgaggatc tggtctgcgc
     4381 aatgatgttg cacattcgcc agcaggcgca ggcgcagctg ccagaagcga ttactcaggc
     4441 ggtgattggt cgcccgatca acttccaggg gctgggcggc gatgaagcaa acgcccaggc
     4501 gcaggggatt ctggaacgcg cggcgaagcg tgccgggttt aaggatgtgg tattccagta
     4561 cgagccggta gcggctgggc tggattacga agccaccttg caggaagaaa aacgggtgct
     4621 ggtggtggat atcggcggtg gtacgactga ctgttcattg ctgctgatgg ggccgcagtg
     4681 gcgttcgcgt ctcgatcgtg aagccagtct gctgggtcac agtggttgtc gtattggcgg
     4741 taacgatctg gatatcgccc tggcgtttaa aaacctgatg ccactgctgg gtatgggtgg
     4801 cgaaaccgaa aaaggcatcg ccctgccgat cctgccgtgg tggaatgcgg ttgccatcaa
     4861 cgacgttcct gcacagagtg atttctacag tagtgccaac ggtcgtctgc ttaacgatct
     4921 ggtacgcgat gcccgcgaac cggaaaaagt ggccctgtta cagaaagtct ggcgtcagcg
     4981 tttaagctat cgcctggtgc gtagcgcaga agagtgcaaa attgctcttt caagcgtagc
     5041 ggaaacccgc gcctcactgc cgtttatcag caatgaactg gctacgctga ttagccagcg
     5101 agggctggaa agcgccctca gccagccact ggcgcggatt ctggaacagg tgcaactggc
     5161 cctggataac gcccaggaaa aaccggacgt tatctacctg accggcggta gcgcccgttc
     5221 tccgctgatt aaaaaagcgc tggcagaaca gttgccgggc attccgattg caggcggcga
     5281 tgactttggc tccgtcaccg ccgggctggc acgctgggcg gaagtggtat ttcgttaaaa
     5341 gaaaatggct cagcagcggc ttaaatcagc ctgcgcctga gccagttttt ctgccgcctg
     5401 acgtaacggt tccatcaacg gcatggtccg tgctggagca tgaaggcgga atcgctgcaa
     5461 ctctgtcgcg ccgacagtca acgtcgattc cagccgatgt cgtttggcgg ccattttcgc
     5521 cattaccgcc tgtctgtctg ctggcgttac cgcttcgttc ggcctgaaca caaaacggcg
     5581 ttcacagctc gctttccagt cgatgaccgc ctgggtcaga tgatcaccaa accctttaac
     5641 ttgcttaacg ccacgacggg taacacctgc tgctgtttca ataccaaaag accataacgc
     5701 cgctttacgc gcagggccaa cgccgggaat agaggcaaca tcaataaaaa atccctccag
     5761 aaacttctgc ttctgccgtt cccttgcagt gtcatgaagt gctgccagag cgagtttctc
     5821 ttcttccggt aacccgagaa tttcgtcctt cattttttcg agcatcgtcc gtttggcgat
     5881 aaaaccttcc aggccgccta cctgctggat ctggcggact aaatggtcat agtccatttt
     5941 cgcacgatta aatgcctcac ggcgttgctg aacttctgct ttgtacgctt tgcttgtcag
     6001 actgccaata atccagatag ccgccagcac aggaaccaaa ataatataac gcggttctgc
     6061 ctgaaggccg caaagcagca gcgataatgc tgacagtgcg atctcaagca gaatgatgta
     6121 ttcgcgccgt aacaggccta aaggaagcgg cctgccagtc ggttggaaat gatcgggtaa
     6181 tggcagttgc aatgctggcg gtgctactga cgccatcacc atcgcccaga cttttgccag
     6241 cacaaaatcc ccgccggtgg taatgacctc ttcgccgaga tcaataaaat agataacgcc
     6301 ttgattatcc agcgtacacc acgggcagtc ggtcaaatga gcgggataaa catgcattgc
     6361 cgaaacggga catttcttta actgttggcg cagagaatca agtgccgcta cccacgcttt
     6421 agccgtcgga cgcccggttg ccacgccact ttccgtgaat gcctgctgaa acatggcttc
     6481 aacatcactc ggtaacatcg atagcggaat cgatcgtggc ggcggtttta aaccacgtcg
     6541 ctgattatct gacgcgtagg cataacggaa atgggtaata tccgtctcca gcgcattacc
     6601 cgcatcagag ataagcggca caccagaata aggatgccgg ccaccaaaca agacgtgaaa
     6661 aatcagcaac gcaaggccaa aattatcgtg attttctgtg cgttcaaagc cgacaaatga
     6721 cgacaaggtt tgtagctctg gcggcgtaaa atgcgacacg ccgacttcgc ataaatgcag
     6781 tgtgccattg gcgttaatct gaaaggagtc gctatcaatc aacaccactt tgctgtcgcg
     6841 acctaccata aaactgttct ggtttacgtc acccacgaca tgcccgtgct cgtgaaccgt
     6901 agcaaaagat gaagcaatat tgcgcgcaac atagagtaga aaatcccacg cacaatgagg
     6961 gtaacgttga cgacgatgtg ccgggctata gatcatatga atcggttctt taccagaaac
     7021 ttttggcatc ataaaaccga taacttttcc gcctcgtcca ccgtgaagcg ttgcctgcgg
     7081 ccaggcgaca taattcaaca attgcgcgtc agctgtcgca gccataaagg caagtttgtc
     7141 ctgttttaag gcgggtggcg gcgtgtgata aatcttggcg acgctatcga caaactcatt
     7201 gatatcataa accgcgcctt caccgccttt gcccagttca cggcctggcg taacgcactc
     7261 accagtagca gtatataaag tgggtttcat ggtaaccaca gcgccagtgc caacgtttta
     7321 tcatcatccg tacgctcgtt taccgccggg ctggacaaaa actgctttaa caattcaggt
     7381 aataaatcga gttgttcctg cgttgctgac gccagtccat tgaaaaacgg ggtaaagaaa
     7441 ggcacatgcg gagaattatc caacatattt aacgccagtc gctggatacc atccgtaaat
     7501 gctgcaactt tatgcacacg ctcagtgctg gtgaaagttt ccagtctgga aacggcatct
     7561 tcatcggtaa tgaagtgcgt catattggca tattcaccca ccatcggcgt aagcggcaat
     7621 tgcagaccgt gaccaaaatc caccaccacg ccgccatcgc caatctgcat aatcaaggtg
     7681 ccattagccg atgaaatcaa tcccagaaac gtgcaggcaa aatcgcgaac tgctaattct
     7741 ttggcttccg cctcggcaaa aagtcgctga cgaacggtca gcacaatatc cgtggcgagg
     7801 atatcattaa gccccaattc cccaccctgc actttttgcg acatataagc catcgcttca
     7861 ttgacggcga gcatcgcgcc ttcgcccccc tgcgaaacgc tacctgcgcc atcagcaagg
     7921 aacatcacca gtaatggctg ctggtcattt agccaggcaa cctgcatctg gcaggcatcc
     7981 tggcaaggca agtccgcgct gatgtgcgac gttccaacag cggatgcata aaccagacgc
     8041 cagctcacac tgacgtccag ccttttggcg cttccagtac aacttccgtt cccggcgtgg
     8101 agcgggaaac cgaacgcagc gaactggata accagctaaa cagttcacgg aattgtaatc
     8161 cttgcagagg caaaggctga cgaacgctga tttgtgccag agtcttcata tccgccccct
     8221 gaacaccgat ggaaaagaaa gcaaaccttt tatcttcttc cccccgaaac actttgttgg
     8281 ctgcagcctg ccactcatcg gttggtgcac catcggtaat caggaaaatc cacgggcgat
     8341 aatacgaaat accatttgca cgatactcac gtttccgctc ttctaccata tccagggctt
     8401 tggtaatggc cgcacccatt ggtgtatcgc cctgggcaaa caggatgggc ggaaaaaaat
     8461 tagcggcgct ggtaaagggt tgttccacat gcaccgggcc gaacgtcaca atgccgagtt
     8521 ccactctttt taaagccagc gaatcggcaa gcagctcatc gcgaaaggta accaatccgg
     8581 tgttaagttc attaatagtt ctgccactca tcgagccaga aacatccagc agcagaatgc
     8641 aggggcaacg tggttccggg ttactggcaa aatcgctggt ggcaaacgtg atttgttcgc
     8701 tcataagaaa tcctttctta gagaattatt gaggtccggt atcataccga ctgcaataat
     8761 gaaataagag caaacgaaga aactttcaga attagtcccg gcggcgcaga aaattttgct
     8821 attcgcctcg caagaacgga gagccgtcga ttgacactaa gggcggagtg acataatttc
     8881 aggagtgagg gttagggaga ggt
//
LOCUS       DBKEKI010000158         8824 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.159, whole genome shotgun sequence.
ACCESSION   DBKEKI010000158 DBKEKI010000000
VERSION     DBKEKI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.159"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1751
                     /gene="traD"
                     /locus_tag="ACUQPX_003640"
     CDS             <1..1751
                     /gene="traD"
                     /locus_tag="ACUQPX_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HHV9058196.1"
                     /translation="QLTDNPKDVARMLKKDGRDSDIRIGDLPIIRDSEIQNFCLHGTV
                     GAGKSEVIRRLANYARQRGDMVVIYDRSGEFVKSYYDPSIDKSLNPLDARCAAWDLWK
                     ECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLS
                     IKIEKLRTYLRNSPAANLVEEKIEKTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRD
                     WMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDE
                     LPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRS
                     PSHKIAEFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPD
                     LTCYVTLPGPYPAVKLSLKYQERPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMA
                     SLFEPDVPEVVSGEDVTQAEQPQQPQQPQQPQQPQQPQQPVSPVINDKKSDSGVNVPA
                     GGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNI
                     NVHRERGEDVEPGDDF"
     gene            complement(1760..2158)
                     /gene="vapC"
                     /locus_tag="ACUQPX_003641"
     CDS             complement(1760..2158)
                     /gene="vapC"
                     /locus_tag="ACUQPX_003641"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HHV9058197.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(2158..2385)
                     /gene="vapB"
                     /locus_tag="ACUQPX_003642"
     CDS             complement(2158..2385)
                     /gene="vapB"
                     /locus_tag="ACUQPX_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HHV9058198.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            2467..7744
                     /gene="traI"
                     /locus_tag="ACUQPX_003643"
                     /pseudo
     CDS             2467..7744
                     /gene="traI"
                     /locus_tag="ACUQPX_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            7764..8510
                     /gene="traX"
                     /locus_tag="ACUQPX_003644"
     CDS             7764..8510
                     /gene="traX"
                     /locus_tag="ACUQPX_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HHV9058199.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            8565..>8824
                     /gene="finO"
                     /locus_tag="ACUQPX_003645"
     CDS             8565..>8824
                     /gene="finO"
                     /locus_tag="ACUQPX_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HHV9058200.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPG"
BASE COUNT         1994 a         2289 c         2796 g         1745 t
ORIGIN      
        1 gtcaactgac agacaatccg aaagacgttg cccggatgct gaaaaaagac ggcagggact
       61 ccgatatccg gattggcgat ctgccgatta tccgggattc cgaaatccag aacttctgtc
      121 tgcacggcac ggtcggggcc ggtaagtcgg aggttatccg tcgtctggcc aactacgccc
      181 gtcagcgtgg tgatatggtg gtgatttatg accgttcagg ggaatttgtt aaaagttact
      241 atgatccctc catcgataag agcctgaatc cgctggatgc acgctgtgcc gcctgggatt
      301 tgtggaagga gtgcctgaca cagccggatt ttgataatac cgcaaatact ctgatcccga
      361 tgggcacaaa agaggacccg ttctggcagg gttcaggacg taccattttt gcggaagcgg
      421 cgtatctgat gcgtaatgac cccaaccgca gctacagcaa actggtggac acactgcttt
      481 ccatcaaaat tgaaaaactg cgtacctacc tgcgtaattc accggcggcc aacctggtgg
      541 aagagaaaat cgagaaaacg gccatctcca tccgtgctgt gctgaccaac tatgtgaaag
      601 ccatccgtta cctgcagggg attgagcata acggtgagcc cttcaccatc cgtgactgga
      661 tgcgtggtgt ccgggaggat cagaaaaacg gctggctgtt tatctcgtcg aatgccgaca
      721 ctcatgcctc cctgaagccg gtgatctcca tgtggctgtc cattgccatt cgtggtctgc
      781 tggcaatggg agaaaaccgt aatcgtcgtg tgtggttctt ctgtgatgag ttacccacat
      841 tacacaaact gccggacctg gtggagatcc tgccggaagc ccgtaagttc ggtggctgtt
      901 atgtgtttgg tatccagtcc tatgcccagc tggaagatat ctacggtgag aaagcggctg
      961 ccacgctgtt tgacgtcatg aacacccgtg cctttttccg ttctcccagc cataagattg
     1021 cggagttcgc tgccggtgaa attggtgaga aagagcacct gaaagccagc gaacagtatt
     1081 cctacggtgc tgatccggta cgtgacgggg tatcgaccgg taaggatatg gagcgccaga
     1141 cgctggtcag ttattccgac attcagtccc tgccggatct gacctgctat gtcaccctgc
     1201 ccggaccgta tccggcagtc aaactctcac tgaaatatca ggaacgtccg aaggtcgctc
     1261 cggagtttat tccgcgtgac atcaacccgg aaatggagaa ccgtctgagt gccgtacttg
     1321 ccgcaaggga agcagaaggt cgtcagatgg ccagcctctt cgaaccggat gtcccggagg
     1381 ttgtttccgg agaagacgtg actcaggctg aacagccgca acagccgcaa cagccgcaac
     1441 agccgcaaca gccgcaacag ccgcaacagc cggtgtctcc tgtcatcaac gataagaagt
     1501 cagattcagg tgtgaatgtt ccggcagggg ggatcgagca ggagctgaaa atgaaaccgg
     1561 aagaagagat ggaacagcaa ctgccacccg ggatcagtga atccggtgaa gtggtggata
     1621 tggccgctta tgaggcatgg cagcaggaaa atcatccgga cacctggcag cagatgcagc
     1681 gtcgtgaaga ggtgaacatc aatgtgcacc gggaacgcgg ggaggatgtt gagccgggag
     1741 atgatttctg atgaacaggt cagctccagt cttcagttct caggccgccc acacgttcaa
     1801 attcccgggt gttattagtc acaataatca gcccccgact gcgggcatga cctgcgatca
     1861 tttgatcaaa tggcccgaca gggcgtcctt gacgggcaag ttctgctctt atctggccgg
     1921 tgtgtgtggc tgcagcagcg tcgtaatcca gaacatcaat gcgggaaaca aatccctcga
     1981 tcacagcgag attacgttca ggcatctggc ttttttctgc accatatatc agctccatca
     2041 gggtgaccga actgatacac attctcccct ggttcaggtt aaaacgctcc ctgacgctgg
     2101 cgggtttgtt ctttatcgta aaaatgcaga tgttggtatc gagcataaac ttcagcatca
     2161 gaatgactcc ctttcctgca tgccgggctg ttccctgtta tccataaaat cggcgctgac
     2221 gctgtgtccg tcgaaccatt cgtcccatgt ctctccggct ggcgtaatga ttctggttcg
     2281 tccgacagca atcacttcaa cgcgttttac gttttccggc aatgcaaccg cttttggcag
     2341 tctgaccgcc tggctgcggt tgctgagaaa tacggtggtt tccattattt gcctccttat
     2401 gtggatatgt cttgtgtata tctattgtgg gattgatacc gggatatgtc aaagggatat
     2461 acgtttatga tgagtatcgc gcaggtcaga tcggccggaa gtgccggtaa ttattatacc
     2521 gacaaggata attactatgt gctgggcagc atgggagaac gctgggccgg ccagggggct
     2581 gaacaactgg ggctgcaggg cagtgtcgat aaggatgtct ttacccgcct tctggagggc
     2641 aggctgccgg acggggcgga tttaagccgc atgcaggatg gcagtaacag gcatcgtccc
     2701 ggctacgacc tgaccttctc cgctcccaaa agtgtctcca tgatggccat gctcgggggg
     2761 gacaaacgtc ttattgaagc acataaccag gccgtggatt ttgctgtccg tcatcacccg
     2821 tgtggaggca ctggcctcca cacgggtgat gacggacgga cagtcagaaa cggtgctgac
     2881 cggtaatctg gtgatggcac tgtttaacca cgacaccagt cgcgatcagg aaccacagtt
     2941 acacacgcat gcggtggtga ctaatgttac gcagcataac ggcgaatgga agacactgag
     3001 cagtgacaaa gtggggaaaa cggggttcat tgagaatgtg tacgctaatc agattgcctt
     3061 tggcaggctc taccgggaaa aactgaaaga gcaggttgag gcgctgggct atgaaactga
     3121 agtggtcggt aaacacggta tgtgggaaat gccgggtgta ccggtggagg ccttttccgg
     3181 acgctcacag actatccggg aggccgtcgg ggaagacgcc tcgctgaaat cccgggatgt
     3241 ggcggcgctg gatacgcgta aatccaaaca gcacgtcgat cctgaagtca gaatggccga
     3301 gtggatgcag acgctgaagg aaaccgggtt cgacatccgg gcatatcgtg acgcagcgga
     3361 acaacgggca tatacccgca cgcagacacc cggacccgct tcacaggacg ggccggatgt
     3421 gcagcaggcg gtgacacagg ccattgccgg attaagtgaa cgcaaagtgc agtttatgta
     3481 tacggacctg ctggccagga cggtcggcat actgccgccg gaaaatggtg tgattgaacg
     3541 ggcacgcgcc ggtatcgatg aggccatcag ccgtgagcag cttatccccc tcgatcgtga
     3601 gaaggggctg ttcacgtccg ggattcatat gctcgatgag ctgtcagtcc gggcactcag
     3661 tcgtgacatc atgaaacaga accgggtgac cgtacatccg gagaaaagtg tcccccggac
     3721 ggccggttac agcgatgccg tcagtgttct ggcacaggac cgtccgtcgc tggccattgt
     3781 gtccgggcag ggcggtgcag ccgggcagcg tgagcgggtg gctgaactgg tcatgatggc
     3841 ccgggagcag gggcgggagg tgcagattat cgctgctgac cgtcgctcgc agatgaacct
     3901 gaagcaggat gaacggctgt ccggtgaact gataaccgga cgtcgtcagt tgctggaagg
     3961 catggccttc acgccgggca gtaccgttat cgtcgaccag ggcgaaaaac tctccctgaa
     4021 agagacgtta accctgctgg atggtgccgc acgtcataac gtacaggtcc tgataaccga
     4081 cagcgggcag cgaaccggta caggcagtgc actgatggcc atgaaggatg ccggggtgaa
     4141 cacatatcgc tggcaggggg gagaacagcg accggccacc atcatcagtg aaccggaccg
     4201 taatgtccgc tatgcccggc tggccggaga ttttgcggcc agcgtgaaag ccggagaaga
     4261 gagcgtggca caggtcagcg gagtacggga acaggccata ctgacacagg tcattcgcag
     4321 tgagctgaaa acacagggcg tgctcggaca cccggaggta accatgaccg ccctgtcacc
     4381 ggtctggctg gacagccgga gccgttatct gcgggatatg taccgcccgg gaatggtgat
     4441 ggagcagtgg aacccggaga cacgcagtca tgaccgctat gtgacagaac gtgtgacggc
     4501 gcagagtcac agcctgaccc tgcggaatgc gcagggcgaa acgcaggtgg tgcgtatttc
     4561 ttccctggac agcagctggt cgctgttccg gccggaaaaa atgccggtgg cagacggtga
     4621 gcgtctgagg gtgacaggga aaattcccgg actccgcgtc tccggcggtg accgcctgca
     4681 ggtggcatca gtcagtgaag atgcgatgac ggttgttgtg ccgggacggg ctgaaccggc
     4741 caccctgcct gtgagcgatt caccgttcac ggcactgaag ctggagaacg gctgggtgga
     4801 aacgcccggg cattccgtca gcgacagtgc gacggttttt gcctccgtca cacagatggc
     4861 aatggacaat gccaccctga acggtctggc ccgcagtggt cgtgatgtcc ggctgtattc
     4921 ctcactggat gaaacccgta ctgcggaaaa acttgcccgc catccctcct ttacggtggt
     4981 ttctgagcag ataaaggcgc gtgccggtga gacattgctg gaaaccgcta tcagtctgca
     5041 gaaagccggg cttcacacgc cggcacagca ggccattcat ctggcccttc ctgtgctgga
     5101 aagtaaaaac ctggccttca gcatggtgga cctgctgaca gaggcgaagt cgtttgctgc
     5161 agaaggaacc ggttttgctg acctgggagg ggaaatcaat gcgcagataa aacggggtga
     5221 tttactgtat gtggatgtgg caaaaggcta tggcacaggc ctgctggttt cccgtgcgtc
     5281 gtatgaggca gaaaagagca ttcttcgcca tattctcgaa ggtaaggagg cggtcacgcc
     5341 gctgatggag agagtacctg gcgaactcat ggagaaactg acatcaggac agcgcgccgc
     5401 cacccgtatg atactggaaa cgtccgaccg tttcacggtg gtgcagggct atgccggtgt
     5461 gggtaagacc acacagttcc gggcggtgat gtcagccgtg aacatgctgc cggagagtga
     5521 acgtccccgt gtcgtggggc tggggcccac acaccgtgcg gtcggggaga tgcgcagcgc
     5581 cggcgtggat gcacagacac tggcgtcctt tctgcatgac acgcagctgc agcagcgcag
     5641 cggagaaacg ccggatttca gcaacacgct gttccttctc gatgagagct ctatggtggg
     5701 caataccgac atggcacggg catacgccct gattgcggcc ggtggcggtc gggctgtggc
     5761 cagcggtgac acggaccagc tgcaggccat cgcgcccggt cagcctttcc gtctccagca
     5821 gacgcgcagt gctgccgatg tggtcatcat gaaggagatt gtgcgtcaga cgccggaact
     5881 gcgggaggcg gtatacagcc tgattaaccg ggatgtggaa agggcgctgt ccgggcttga
     5941 gcgtgtgaaa ccgtctcagg tgccacgtct ggagggcgca tgggcaccgg agcactccgt
     6001 gacggagttc agtcacagcc aggaagcgaa actggcagaa gcgcagcaga aggcgatgct
     6061 gaaaggcgag gcttttccgg atgtccccat gacactgtat gaagccattg tccgcgacta
     6121 taccggcagg acaccggaag cacgggagca gacgctgatt gtcacgcacc tgaatgagga
     6181 ccggcgcgta ctgaacagca tgattcatga tgcacgggaa aaggccggag aactggggca
     6241 ggtgcaggtc atggtgcctg tcctgaacac agcgaatata cgcgacgggg agctgcgtcg
     6301 tctctccacc tgggagaata acccggatgc ccttgccctg gtggataatg tgtatcaccg
     6361 gattgccggt atcagcaagg atgacgggct gataaccctg caggatgcgg aaggtaacac
     6421 gcggctgatt tcgccccggg aggcggtggc tgaaggcgtc acgctgtaca ccccggacac
     6481 catccgggtg gggaccggtg acaggatacg cttcacgaag agtgaccggg agcgcggtta
     6541 tgtggccaac agcgtctgga cggtgacagc ggtttccggt gacagtgtca cactttctga
     6601 cggtcagcag acccgtgtga ttcgccccgg tcaggagcgg gcagagcaac atattgacct
     6661 ggcctatgcc atcaccgccc acggtgcaca gggggcgagt gaaacctttg ccatcgcgct
     6721 tgaaggcacg gaaggtaacc ggaaactgat ggccggcttt gagtcagcct acgtggccct
     6781 gtcgcgtatg aagcagcatg tgcaggtgta caccgataac cgtcagggct ggacggatgc
     6841 catcaacaat gccgtacaga aaggaactgc ccacgatgtg tttgagccga aaccggaccg
     6901 ggaggtcatg aatgcagagc ggctgttcag tacggcgcgg gagctgcggg acgtggcggc
     6961 aggccgtgcc gttctccgtc aggcggggct ggccggggga gacagtcctg cacggtttat
     7021 tgctccggga cgtaaatatc cgcaaccgta tgtggcactg ccggcgtttg accgtaacgg
     7081 caagtccacc ggtatctggc tgaacccgct gaccacggat gacggaaacg ggctgcgggg
     7141 attcagcggt gaaggtcggg taaaaggcag cggggatgcg cagttcgtgg ccctgcaggg
     7201 cagccgtaac ggagaaagcc tgctggctga taatatgcag gatggcgtcc ggattgcccg
     7261 tgataatcct gacagtggtg tggtggtaag aatcgccggt gaaggccgtc cgtggaatcc
     7321 cggtgccatc accggtggcc gcgtgtgggg ggatatcccg gacaacagcg tccagccggg
     7381 agccggaaat ggcgaaccgg tcacggcaga ggtactggca cagcggcagg ctgaagaggc
     7441 catccgccgt gaaacggaac gtcgcgcaga tgaaattgtc cgtaaaatgg cagagaacaa
     7501 acctgacctg ccggacggca aaacagagca ggctgtcagg gaaattgccg ggcaggagcg
     7561 tgaccgggct gccataactg aacgggaagc cgcgctgccg gagagtgtac tgcgtgaacc
     7621 acaacgggtg cgggaagcgg tccgggaagt tgcccgggaa aatttgctgc aggagcgtct
     7681 gcagcagatg gagcgggata tggttcgtga cctgcagaaa gagaaaaccc cgggcggaga
     7741 ctgatacagg aagacaaaca ctgatgacaa cggataacac gaacacgaca cgtaacgatt
     7801 cactggctgc acggaccgat acctggttgc agtcatttct ggtctggtca cccggacagc
     7861 gggatatcat caaaacggtg gcactggtgc tgatggtact ggaccacatc aatctgatat
     7921 tccagctgaa gcaggaatgg atgtttctgg ccgggcgagg agcctttccg ctttttgccc
     7981 tggtgtgggg gctgaatctg tcccgtcatg cgcatatccg gcaaccggcc attaaccggc
     8041 tgtggggatg gggaatcatt gcccagttcg catattatct ggcaggcttt ccctggtatg
     8101 aggggaatat cctgtttgcc tttgcagtgg cggcacaggt gctgacgtgg tgtgaaacgc
     8161 gcagtgggtg gcgtacagca gcggccatac tcctgatggc cctgtggggg cctttgtccg
     8221 gcaccagtta cggtattgcc gggttgctga tgctggcagt cagcaaccgg ctgtaccggg
     8281 cggaagacag agcggaacgt ctggcgctgg tcgcctgcct gctggctgtt attccggcgc
     8341 ttaaccttgc caccagtgat gcagcggcgg tggccggtct tgtgatgacg gtgctgaccg
     8401 ttggtctggt gttgtgtgcc ggaaaatcat taccccgttt ctggcccgga gactttttcc
     8461 cgacgttcta tgcctgtcat ctggctgtgc tgggcgttct ggcgctgtga cgggtgtggt
     8521 atctttggcc gcaagaggat gattcgtcag aggcagaaca cagcatgaca gagcagaagc
     8581 gaccggtact gacactgaag cggaagacag agggaacagc gcctgtccgc agccggaaaa
     8641 ccatcatcaa tgtcaccacg ccaccaaagt ggaaggtgaa aaagcagaag ctggccgaga
     8701 aagcggcccg tgaagcagag ctggcggcaa aaaaagcgca ggccagacag gcgctgtcca
     8761 tttatctgaa cctgcccacg ctggatgagg ctgtgaatac cctgaagccc tggtggccgg
     8821 gatt
//
LOCUS       DBKEKI010000159         8773 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.160, whole genome shotgun sequence.
ACCESSION   DBKEKI010000159 DBKEKI010000000
VERSION     DBKEKI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.160"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..945)
                     /gene="istA"
                     /locus_tag="ACUQPX_003646"
                     /pseudo
     CDS             complement(1..945)
                     /gene="istA"
                     /locus_tag="ACUQPX_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
     gene            complement(1032..1393)
                     /locus_tag="ACUQPX_003647"
                     /pseudo
     CDS             complement(1032..1393)
                     /locus_tag="ACUQPX_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1733..2287)
                     /locus_tag="ACUQPX_003648"
                     /pseudo
     CDS             complement(1733..2287)
                     /locus_tag="ACUQPX_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            2430..2552
                     /locus_tag="ACUQPX_003649"
                     /pseudo
     CDS             2430..2552
                     /locus_tag="ACUQPX_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2814..3380)
                     /gene="ipgB2"
                     /locus_tag="ACUQPX_003650"
     CDS             complement(2814..3380)
                     /gene="ipgB2"
                     /locus_tag="ACUQPX_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HHV9058201.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYCISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(3662..3976)
                     /locus_tag="ACUQPX_003651"
     CDS             complement(3662..3976)
                     /locus_tag="ACUQPX_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058202.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(4054..4164)
                     /locus_tag="ACUQPX_003652"
                     /pseudo
     CDS             complement(4054..4164)
                     /locus_tag="ACUQPX_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4488..5165)
                     /gene="ospD1"
                     /locus_tag="ACUQPX_003653"
     CDS             complement(4488..5165)
                     /gene="ospD1"
                     /locus_tag="ACUQPX_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HHV9058203.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMISNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(5536..5673)
                     /locus_tag="ACUQPX_003654"
                     /pseudo
     CDS             complement(5536..5673)
                     /locus_tag="ACUQPX_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5758..6053)
                     /locus_tag="ACUQPX_003655"
                     /pseudo
     CDS             complement(5758..6053)
                     /locus_tag="ACUQPX_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6534..7929)
                     /gene="tnpC"
                     /locus_tag="ACUQPX_003656"
                     /pseudo
     CDS             complement(6534..7929)
                     /gene="tnpC"
                     /locus_tag="ACUQPX_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8054..8401)
                     /gene="tnpB"
                     /locus_tag="ACUQPX_003657"
     CDS             complement(8054..8401)
                     /gene="tnpB"
                     /locus_tag="ACUQPX_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HHV9058204.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(8401..>8773)
                     /gene="tnpA"
                     /locus_tag="ACUQPX_003658"
     CDS             complement(8401..>8773)
                     /gene="tnpA"
                     /locus_tag="ACUQPX_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HHV9058205.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
BASE COUNT         2224 a         2075 c         1968 g         2506 t
ORIGIN      
        1 tcaccaggat gcacgtcata ctcttttttc tccggaggca gtgccagcat ggactgctgc
       61 tcttcgagcc agcgatcaca gggacgggcc tagattgttt catgctttcg ttggttagcg
      121 acatcgtgca gccagcgcag accgtggcgg ttggctgttt caacatcgac agtgcttccc
      181 catcgggcgc aggcgagtca ttagtgggat gtaaaaactg ttacgggtgt actgcaccat
      241 ccgttccacc ttacctttag tctgtgccct gaaggggcga cacagtcggg gagagaagcc
      301 catctccttg ccgaactgcc acagcgaagg atggaaccgg tgctgaccgg tctgatatgc
      361 gtcacgttgc agaaccacag ttttcatatt gtcatacaac acttcgcgcg gcacaccacc
      421 aaagaagcgg aacgcattac gatggcaggt ctccagcgtg tcataacgca tattgtcagt
      481 gaattcgatg tacaacattc ggctgtatcc gggaacagca acgaacacgt gaagcggtga
      541 gcgaccatta cgcatagtgc cccagtcaac ctgcatctgt cgtccgggtt cagtttcgaa
      601 ccgaacggca ggctcctgct cctgaggaac cgagagagaa cgaatgaatg ccctgagaat
      661 ggtcattccg ccacgatatc cctggtctct gatctcgcga gcgattaccg ttgccgggat
      721 tttgtaagga tgagcatcgg cgatgcgttg acgaatataa tcccggtatt catccaggag
      781 tgaagcaaca gcaggtcgcg gcgtatattt tggcggctca gattttgcct gcaaataacg
      841 tttaacggta ttgcgggaga gccccagttc tctggcaatc gcccggctac tcattccctg
      901 cttgtgcagg attttaattt ccataactgt ctcaaaagtg accataagct ctcctgaatc
      961 aggagagcag attaccccct ggatctgatt tcaggcgttg ggtgtggatc actattgcac
     1021 cgttcgtgac agtgttgttg cgccttgctg ccaacgaagc tccactgccc atcaagttca
     1081 cagataaggg caacatcagc gtgagcgact ggcgcagatg ttatccgccg gggcgggagc
     1141 tttttaatgt ccgtatgacg gtattgatgc cgattttcag cgtacgggca gtatcgcgaa
     1201 ctccggcacc gttgaaagcc atttcagtga tttggttttt aactcccggt ttacgggcct
     1261 cgtaacagta agtgagctga aacacccggt gacagtcacg gcaacggaat cgatcatgtc
     1321 cttttgggtt ctgcccgtga cggtaaacct gaacggactg gcaacggggg caatgaaagt
     1381 taactgtgac catgaaagaa ccttaaaagc gggtattata acccctaatt caactaattg
     1441 gaggcattgt catttttaat ctatggatac acttaaaaat cctctcacct tacgttaaaa
     1501 accttctcat gtttagagtt ttttattatg tttatcataa tgatatgacg atctgagttt
     1561 ttaaaatcaa gaagaaaaga taagtttttt aaatgcacag tgcctgtgga taactttgcg
     1621 taaatccgga ggacagatca ctctgaagca agacaacagc atctgaaaac aatatgctca
     1681 cgtttcaacc acagggcgta agtgatccta cccacgtaat atggacacag gcctaagcga
     1741 ggttctggtt ttcaaattgt tccggactga ggccgccaca ccaactgtgc cgtcgccagc
     1801 gattgtaatc acattcgata taattaaaca ctgttgcccg cattatttcc cggctgataa
     1861 agtgttctcc gtggagacat tccactttca gcgaatgaaa gaagctttcc acgcaggcat
     1921 tatcgtagca gcagcccttt gcgctcatac tgccacgcag attatgccac ttcagcagcg
     1981 cctgataatc tgctgaacag tactgacttc ctcggtccga gtgaacgata acgttccggg
     2041 gtctcttacg ccgccacagc gccatctgca gggcatcgca ggccagttgc gccgtcaggc
     2101 gtggtgacat ggaccagcca ataacggcac gtgaccacag gtcaatgacc acagccagat
     2161 acagctagcc ttcatctgta cgtaagtacg tgatgtctcc cgcccacttc tggttcggac
     2221 cactggcgta aaaatcctgc tccagcagat tttctgacac aggcaggcca tgtgcgcggt
     2281 aactgaccgt attgctgggt aagatcaatt tgctcactgg tatttactac gtgcatcgaa
     2341 tgagtgatca agagccgtat gacgcgagag tgtcacgtac ggttccgtga gcagccggag
     2401 agtgaaactc tctccggctg actcgactcg caggttccga cagcggtgac gaacatgcag
     2461 cggtgttgtt ctcgctgatc ggcacatgcc gtctgaacaa tgtggagcca gaaaaatggc
     2521 tgcgttacgt tattgaacat atccaggact gaccggcaaa ccgggtacgc gatctgttgc
     2581 cttggaaagt tgatctgact tctcagtaaa tatcaatatg attctgacga gccgcttacc
     2641 tttgagcaga gaaaggggag agtgatccca catccatgag ctaggaaaat acaaaaactg
     2701 gctatacaac aattcaatat attaggactt acttgcgagg agaaatatag caccgctcag
     2761 gcaattccta ggtatttact tttcttagtt tctttactcg ggatgttctg tcatcagaaa
     2821 ggcgattcta aatttgtaat atagtcactc atattcccac gtaatttctt tagatcaata
     2881 tattgcacat ttttctcaaa aaactgatcg gaaataagat ttgcaacatt gcttgttgta
     2941 tcatttttaa cgcttctatt catcccttca caaagttcgt ccattttctt tttgaaataa
     3001 ctgcttcctg atacaagatg atttatggaa ctttgtgcag cattgatgtc tagagaaatg
     3061 caataatctt tctcaacctg tttaaatatc tgtttacgga gattatcgcc gatttttcgg
     3121 ctgtgttctg cacaaaactt atcaatacat tggtttacca cccgggatac gtagactttt
     3181 ttttcatcat ttttccaatc tgtaatattt tcaccgattt gttctgaaac agagctcaaa
     3241 atactagtat ttattttggt tgatgatatt tttactatcc ttttccccat tgtacagcgg
     3301 ataatctcat ccacctttcc tgaaaaatat cttttgtgag ataaactgat tccaaaatta
     3361 ttaaaagatg ttccaagcat tataatcact ttgtcatgta aaatagtaac attgtcatta
     3421 ttttagctga ttcaaacaat tctgtaaatg ctttgagttc tttactccct tggctcaatg
     3481 atggaccgtc tggaatccca gagactaaac tctctgagaa agagagtatg aaaaaacttg
     3541 gggcagtccc taaatgaagg gaccaacagc ggtaaaataa ccggcatcat tcataatgat
     3601 gccctgttag ttgtagttat gaagatttac gtcattaaaa ttgctgttcg tagtgtcagt
     3661 cctatggtct ggcatcagct gaggatcgta gccgatacat cactggcttc acgacattcg
     3721 tgttgaggct attcatgaaa actccacgct gaaaacgccg ttttgtataa acggtcacgg
     3781 tatgcccgga gccactgttg ctgatgaatt cgataaaacc ttggcgtttc ttgaagctat
     3841 cgttaatgcg gataatgaaa caacgattgg tgagattcgc tcttttgccg acgccctgga
     3901 cgctgtccgg ttcaatcgca acaaaatcaa ccggcagctg agcaaaccca accttgcctc
     3961 tctggcgctg gagcatgaag tgatttggct gggacgtagc cgctgacagc ttctataaaa
     4021 gtatgatcct acccacgtaa tatgaacaca ggcttaagtg aggttctggt tttcaaattg
     4081 ttcaggactg agacctccac accaactgtg ccgtcgtcag cgattgtaat cgcacttgat
     4141 ataattaaac actgtatatt tcatttgtgg agtctccagt ctgagggggc tttatgcatt
     4201 ccgtattggg cactatgatg ccagaaatct gcaaggctcc acaccttcct gtatttccct
     4261 tcctctatca cagtgcactt tacttaatag ggtgggatgt gttcattaca tttattgtgt
     4321 tttttatgat tgtttttgcg tccagtgtcg ttcgtcatgg gaaactggtt ctatgggtag
     4381 tatcgctcag tctagttaac tgtttgtttc tttttagaaa ttaagcagat cacacaattc
     4441 ggggagagat ccttctaagt gcaattcttt gcagctattt agcacactta aaacaactct
     4501 tgtattttgt cagaaataat attaaacatg tcttgttcat tattgttaag tctgtctgct
     4561 atttctgtaa gttttatccc actatgatgg catatattta tatatgccat catagcctcg
     4621 atattccctt tccctgcggc cagaaataat cctggtaacc cctcattatc tcttacatcc
     4681 aatagtttaa tcgtttcttt tggtgataaa tcaaaagttt taataaaatc tccataagct
     4741 tgaatcgcag cggaatgacc ttcttgaaga gctatgaaca aagcagattc cccagcatta
     4801 tttcgtgctg caagaacttc tagtttttcc tctttgggta tttcggctgc caacagcgat
     4861 tcaccgtatt tacgcgctgc atcggcattt ccatgccaaa tcaatgaaaa caaattatgg
     4921 cggttatact ccttattatt ctttgtcgga atggatatgc tctgtatttt ggccaatttt
     4981 ctgaaagaaa caccactata tcccccccct gatttttctt cattgatggc gtgggatata
     5041 gcggtatttg tcacgttaat gatattactt atcattcctt tattttcatt agcagtattg
     5101 ctgcctatta ttaggtgcat atttttgttg tttgctggat gtaatccata gttatttatt
     5161 gacatttttg ttcatccttt ttgctaaaaa aaacaataac ctatttttgt aatgtaatag
     5221 atattagtct attgctaatc taaaaaaacc attgccacat tgaatggcta tattcaccaa
     5281 taaattcttt cgatttcgta tattgacata tcgtagatat atcttatccc taagattcaa
     5341 taatcgagtc cgtttaccat tctgtgcaag cgccttcact cagaagtgac cgtcaaggcg
     5401 atcaccaaac ttgataataa agcagctcat tgccatacgc cagtccttaa ggggcgttgt
     5461 ccatttctgt gatgctgcct agatttaact gccagctccg gcttatctac atctaaatat
     5521 gtgagcctaa taccgctaca ctttggccag actatgtttt tctccgggaa gtgggctaac
     5581 cgtctccata aagtgacgaa cttttttcaa cagtggccat attgaacggc actgatgatt
     5641 acgggttatt gtctcatgaa gagccggcca catcactgac agcagtgact tcaggccttc
     5701 agcaccagac agcctgaacc agttaatcca tcgcccgaca gatttagtaa tctagggctg
     5761 gtttttcttc agctgttgcg gttcgaaagt gccattacga tcacgcagag tacggatttc
     5821 cagaggttca tctccagtag tgataatctt cgttaagcaa ccattgcggg aattgctgcc
     5881 agatctaagc tgatttttct cgcacccaag atgatgagtc atattagcat tgagtacggt
     5941 ttcattttca gcatccaatc aaactgactg agatcttcag gatttttgag acttttggct
     6001 agttcattag ctaaagcccg caacttatta gatgtcaact gtttttcatt cataaattca
     6061 ccttcatttg atatcaaatt aaatagttca acatcagaga attatacaaa cttatgtaca
     6121 ggctcctatg atcttcgaat cctgaagatt atagtgaaaa taagttttca cgttgaaatc
     6181 ccacatagtt agtatatgat aaataatcat ggatgacctg attaacctct tggggtagtg
     6241 cttttataga agaataaata ttctactaat ttaatacatc acctaaaata gtattgtttt
     6301 ttatggttgg ttgaaggtcg tggtgatgat gttagtcctt ttgagaatat tccagtgcca
     6361 gatcttactt ataataaagg ggcttggcag tttggataaa ctattgttct tattccacta
     6421 atacaaactt tttgtaaagt atattaaata tacttcattt catactgaaa tccccggaag
     6481 tattcaatca aacaattaca ccataaaagg aagagggggc cctaattaag ttgtcaagca
     6541 tgtttatgat acctgcaggg tataaatttt ctggctgcaa gcagacacat ataaaacgtc
     6601 tttattgatc ctacccacat aatatggaca cagtcgtaag tgaggctctg gttttcaaat
     6661 tgttccggac tgagaccacc acaccaacgg tgccgtcgcc agcgattgta atcgcactcg
     6721 atataactaa acactgttgc ccgcactatt tcccgatgga taatagtgtt ctccgtggag
     6781 acattccatt ttcagcgaat gaattttcat ctgttgctgt atccagtcat acagtgactg
     6841 catcagtggc gcggctctgg cttttcttgc cgccagacgc tgttctgctg aacatccccg
     6901 gacttctgcc tcgatggcat acagttcacc gatacgctgc agggcttccg tggtgatgtc
     6961 ggttggcact cttgcatgca catcgtggat ttttctccgg acatgagcca tacatgcggc
     7021 ttccgttatt ctgccggatt cgtacaacgc ccggtaacca ccgtaaacat cggcctgaag
     7081 cactccgctg taaccggcca gatgattctg tggatggata cctttccggt ccggtgagta
     7141 cgcgaaccag accgccggag gcatttccga accggcgtta cggtcatcac gaacgtagac
     7201 ccacagccgg gcagtccttg ttttaccgct gcccggctcc tggaccggaa cggggatatc
     7261 atcggcatgg actttaccgg gcatcagcac atactggcgc aggatgtcat acagcggttc
     7321 cagcagttca gcaacagcac ctgtccagcg ccccagtgtg gcacggctca gctccacgcc
     7381 ctgacgacgg tatatttctg actggcggta taacggcaga tagtctgcat attttccagc
     7441 gacaacatgg gccagaagcc ccgctccggc ataactgcgt gcaatgggtt ttgaaggcac
     7501 cggtgcctgc acgatatggt cacaccggca acaggccagt ttcggacgtt gtgtttcgat
     7561 aaccttaaag gcgctgctga taagctccag ctgctctgac acatcacatc ccagagaact
     7621 gagttcgcca ccacaggccg ggcagcattc ctcttccgga cggatgaccc gggtttcacg
     7681 gggaagtgag gcaggtaacg gtttacgggc tgaagactgg cgcaggggag atggcagtac
     7741 cgggtcatat tgctcaccca gcgtttccgc catttcttcc tgaagtgcgc tgattcgctc
     7801 ctgtgcttcc agtatctgcc gttcggtttt tgcgcgaagt ttttctgagc ttttaccgaa
     7861 ctgcatgcgc tgcagtttcg caaccagcgc cttcagccgg ttgatttcgg aagcataagc
     7921 cgccacccgc tgtgagagca ggcggttgta tttcgccatc tggcggatgg tgtcctgttg
     7981 cgtctgcaac agtgcccgca ggcgggcatt ctcatgagca agtgaggtgt ccatatcctc
     8041 actttacaac gggttatatg cggatgccag cgcgttccgt tcgtttcggg tgtttccagt
     8101 cgatgccctc aagaagcatg gataactggg ccggagtaag gtgcaccttg ccgtcacggg
     8161 tgaccggcca gacgaagcgg ccccgctcca ggcgtctggt aaagaggcac agtccgtcac
     8221 tgtcagccca caacactttt atctggtcac cccggcgtcc gcggaagatg aacagatgcc
     8281 cggagaacgg gtcatccttc aggacgttct gaacttttga ggccaggccg ttaaagccat
     8341 ttcgcatatc ggtgatacct gcaaccagcc agatacgcga acctgcaggg aaagatatca
     8401 tcagtggctg ctccctttta tttcgcggat aagtatctgt aataacgccg gcgtcagttt
     8461 acctttaagc ctgagagttc cggccggcag aaccagctca cagcacagac tgtcggacgg
     8521 tgtatttatc tgctctggtt cctgtgccgg agccgggatt ttattatccg gctccggcgt
     8581 taacgtcacg ggaagcagtg caggcatatt ttttccggaa ggcagcaggc cacctttccg
     8641 gtattgatgg cgccagttga agagcaggtt atcgttgatt ccgttttcac gggcgatctg
     8701 cgccacacag gctccgggct gcagtgactg ctccactaag gcgattttaa attcataagg
     8761 aaaattaggc cgc
//
LOCUS       DBKEKI010000160         8583 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.161, whole genome shotgun sequence.
ACCESSION   DBKEKI010000160 DBKEKI010000000
VERSION     DBKEKI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.161"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..111
                     /gene="rcsA"
                     /locus_tag="ACUQPX_003659"
     CDS             <1..111
                     /gene="rcsA"
                     /locus_tag="ACUQPX_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="with RcsB is a positive regulator of capsular
                     polysaccharide synthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HHV9058206.1"
                     /translation="HKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(155..343)
                     /gene="dsrB"
                     /locus_tag="ACUQPX_003660"
     CDS             complement(155..343)
                     /gene="dsrB"
                     /locus_tag="ACUQPX_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HHV9058207.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            506..733
                     /gene="yodD"
                     /locus_tag="ACUQPX_003661"
     CDS             506..733
                     /gene="yodD"
                     /locus_tag="ACUQPX_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HHV9058208.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            1031..1846
                     /locus_tag="ACUQPX_003662"
                     /pseudo
     CDS             1031..1846
                     /locus_tag="ACUQPX_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(1843..3541)
                     /gene="dgcQ"
                     /locus_tag="ACUQPX_003663"
                     /pseudo
     CDS             complement(1843..3541)
                     /gene="dgcQ"
                     /locus_tag="ACUQPX_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(3712..3894)
                     /locus_tag="ACUQPX_003664"
     CDS             complement(3712..3894)
                     /locus_tag="ACUQPX_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodC family protein"
                     /protein_id="HHV9058209.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(3973..4593)
                     /locus_tag="ACUQPX_003665"
     CDS             complement(3973..4593)
                     /locus_tag="ACUQPX_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HHV9058210.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALSGIALVVTVGVYGLVGVIVKIDD
                     LGYWLAEKSSALMQALGKGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAI
                     EHIAGQQSAVVAMILPTVLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4766..5686
                     /gene="yedA"
                     /locus_tag="ACUQPX_003666"
     CDS             4766..5686
                     /gene="yedA"
                     /locus_tag="ACUQPX_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HHV9058211.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(5675..6145)
                     /gene="vsr"
                     /locus_tag="ACUQPX_003667"
     CDS             complement(5675..6145)
                     /gene="vsr"
                     /locus_tag="ACUQPX_003667"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HHV9058212.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(6126..7544)
                     /gene="dcm"
                     /locus_tag="ACUQPX_003668"
     CDS             complement(6126..7544)
                     /gene="dcm"
                     /locus_tag="ACUQPX_003668"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HHV9058213.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(7611..8306)
                     /locus_tag="ACUQPX_003669"
                     /pseudo
     CDS             complement(7611..8306)
                     /locus_tag="ACUQPX_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(8346..>8583)
                     /gene="drpB"
                     /locus_tag="ACUQPX_003670"
     CDS             complement(8346..>8583)
                     /gene="drpB"
                     /locus_tag="ACUQPX_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HHV9058214.1"
                     /translation="WAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWL
                     GALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         2058 a         2315 c         2115 g         2095 t
ORIGIN      
        1 cataaaggta atattaaacg taagatcaaa acgcataata aacaggttat ctaccatgtc
       61 gtccgactga cggataatgt gactaatggt atttttgtca acatgcgcta acacattctg
      121 actggtggtt tccctccagt caggctgaat aagattactc tgctttctca acaaagatac
      181 cgtcctgatg ccctgcttca ttaaagaacc agatgccgag cgggtagtct tccagcgaaa
      241 ccaggtacat tgtgccttca ctaaactcct caactgccag taccactcca ggacgacgcg
      301 gaccgccatc cgttttgact gttacccgat cattcacctt catcgttttc ctcctgtggc
      361 tttgtgccag tgtagaacaa tttcgttttt ctggcagcgc caggcgcgcg cgagtgctga
      421 ttttctcgac ggtctatact taagagatgc caggcggact taacgactgg cggcaacaac
      481 agagtaacgg ttgcgaggaa agatgatgaa aaccgcaaaa gtgtacagcg ataccgcaaa
      541 acgtgaggtc agcgtcgatg tcgatgccct gctggcggcg atcaatgaaa ttagcgaaag
      601 cgaagttcat cgcagccaga acgattctga acacgttagc gtcgatggac gtgaatatca
      661 tacatggcgt gaattggcgg atgccttcga actggatatt catgacttca gcgtctctga
      721 agtgaatcgt tgaatgcaca ataaaaaaat cccgaccctg agggtgtcgg gatgaaactt
      781 gcttaagcaa gaagcactta aaaaattcgt tacaccagga aatctgatgt gttcatcacc
      841 ttatctgcaa tttttttcgc tgacaagaaa atattcgcgc tatgaatgat taacgctact
      901 tatgaataga aatgtgacgc gcgtcacccc ttggtggctc tggtgtcaac gggatcgctc
      961 ccgaaaaaat catctttgat tatttgtgct gttttgagaa tcatcctggt aaggggagta
     1021 ctgcgcgacc atgttttcaa ttcaacaacc actactggtt tttagcgatc ttgatggcac
     1081 cctgctggac agtcatagtt atgactggca accggcagcc ccctggctca gccgtttaca
     1141 cgaagccaat attcccgtca ttctctgtag cagtaaaact tcagcggaaa tgctgtactt
     1201 gcaaaaaatg ttggggctac aaggcttacc gctgattgca gagaatggcg cagtgatcca
     1261 gcttgctgaa caatggcagg atatagacgg ttttccacgc atcatctcag gtattagcca
     1321 tggcgaaatc tgccaggttt taaatacgct acgcgagaaa gaacatttta aattcacgac
     1381 ttttgatgat gtcgacgatg caaccattgc cgaatggacg ggattaagcc gtagctaggc
     1441 ggcgctgacg cagctgcatg aggcgtcggt aacgctaatc tggcgcgaca gtgacgagcg
     1501 tatggcacaa tttatcgctc gtctgaacga actgggcttg cagtttatgc agggtgcgcg
     1561 cttctggcac gtactggatg cctctgccgg aaaagatcag gctgccaact ggattatcgc
     1621 gacctatcaa caattgtcag gcagacgccc aaccacactt ggcctgggcg atgggccaaa
     1681 cgatgcgcct ttactggagg taatggatta cgcggtgatt gtgaaagggc taaaccgtga
     1741 aggggtacat ctgcatgatg aggatccggc ccgcgtctgg cgaacgcagc gtgaaggacc
     1801 ggaaggctgg cgtgaagggc tggaccattt tttctccgcc cgttaagcgt tatcgctcgc
     1861 gaatacccga ttgcgcccgg cctgttttgc gaggtaaagc cgacggtcgg ccaatgactg
     1921 gagttgttca aaatcataat caccggtttc ctcgctgcta cttaccccca gcgaggcact
     1981 gatgcgtatc gttgtactct tggcgatcag catctctttt tcatttaact tcaggcgaat
     2041 acgttctgcg acctccgcag cctgcgtcag attcgcgcct ggcagaatca cacaaaactc
     2101 ctcaccaccg acccgcccgg caacgtcctg cgcacgcaag gaactgctaa ttaatccggc
     2161 agcatgagaa agaacacggt cgcccgcctg atgaccaaag cggtcattaa tcgctttaaa
     2221 atggtcaaga tcgacctgaa tgacagaaaa aggatgttgg tgcgtctgac acaatttagc
     2281 gagcggacgg gctttttcga acagtgcgcc acggttatat aaacgcgtta aggtgtcgtg
     2341 ccacgcctgc cactgcaacg agctttgcag aacatacatg ttgctgacca tccggcgaat
     2401 cacataccag gagaggagta acatggtggt aaagagcgcc cacagcaggg ttaatgcaat
     2461 gctgatactg ccgaaatcgc cgcgcacgcc ttcgcttagc gtatggacgc gcaccagcac
     2521 accgtcgaaa tggtccagac gttcccagct aacatagcga ctgtccatac gaatgccgcc
     2581 ccgcgtgtca tgttccattg cctgcgccag caaggccagt tcacgaggat caaaaatatt
     2641 ccctgttgga tggtcaggat tggaagaggt caaaaatctc agcttactgt catagagctg
     2701 atactcacca tcgaggtttt tatcgatggc gtttcttaaa aattgctgca tagtacgcac
     2761 gggaatactc atccccagca cgccatacca gtagttatta ctgtctaccg gaacactgac
     2821 ggtaacctgc ggttcagtat tgctggcgtg ttccggttgc gaagtgaacc agcgtaccgc
     2881 gcggtgacga ttttctcgtt gcgaatggcc gataaaccag ggttgggtga catagccgta
     2941 ataacgcgtt ggtacattgc gcgtaaacaa ggtcggctgc gtcgaaacgt aaaatccagc
     3001 acgcgagaca tacatcgcct gttcaaccat cgacgaggag ttgtgcgcca gtcgcagcaa
     3061 gtaaccaact tccagtgcag cggtaatttc attgtcgagg ctttcatttt cgcgagacag
     3121 gaaattcccc tcgctgacta atgcatccga tacgccattg actggcaggg tgcgccgtcg
     3181 gttgagttcg atttgccagg cgtgctcgtc gcgatgctgt tcgaactcgg ttacggcatt
     3241 acgcagagag gtgaaatcca gtggcgccac gagagcttcg cgcatgccat tacgcaaaaa
     3301 gatcagtttg tcgacgttcg ttatactgca aatgcttatc gagcgcgttg gctacgtttt
     3361 ccagatgatt acgctggctg gagatatagg catcttccag caccaccact tcacgccagg
     3421 tgagcaaggt ggaaaaaagc aataccacga taaagcagag attaacgaca tgaccgggcc
     3481 ccaggcggcg tgcgagtttt ttcaaccagc tctggttttc catttttgtc tcgtgctgca
     3541 ccctgacgac tcccaacctg cttttttatg attctggcat aacccggcga tagcgtaccg
     3601 caaaacagcg ataattttat aatggtggca tgataagacg cgactatcat tcattgcagg
     3661 cattgggaca ccaggccgga tgtgatccgg cctggtgtta atgataccct ttcaaacttc
     3721 ttccgcagaa cgactcccct cccccggaac aagatcggtt tcatgaaaag cttcccgctt
     3781 gaccccgtaa ccgtcatacc aacgacactc aaccataccg ctggagtatc cagtgacaat
     3841 catccgcggg ccgccctctt taaccgtaac ttcctcacta accataaagc tcatactcgc
     3901 ctcctttttt cgagtgaaac ttgttcacct tagttgaaga tggcgaattt tgcataatag
     3961 ccagtgcgag tattaatgtg cctgaccgcg cattttcgct actgctttca ctcccagcac
     4021 cacgatgccg ccgatgataa atccaagaat cagatttaaa acagtcggta atatcatcgc
     4081 caccactgca ctttgctgcc cggcgatatg ttcaatagcg tgatgcagcg gcgcaatacc
     4141 atgtaccaca atcccgccgc cgacgaggaa catcgccagc gtgccgacaa tcgataatgc
     4201 tttcatcagc caaggcgcga taatcaataa ccctttacct aatgcctgca tcagcgcgct
     4261 ggatttttcc gccagccaat accccaggtc atcaatctta acgataaccc ctaccagacc
     4321 gtagacgccc acggtcacta ccagcgcgat gcctgaaagc gccagcggca ccagaatcac
     4381 tttattgatc agcgatcctt tcgccacgcc ccagaccacg ggaagttccc ggttggcccg
     4441 cacgcctgaa acttgttgcg cattgagcga taaatcatcc cctaatacac cggcggtttt
     4501 cttcgccgcc agtttgccca tcacagagat atcgtccagc agtgtggcga tatcatcgag
     4561 caacgtcagt aaactactcc cggccaaaag cattccctct catttttatt gttgaagggt
     4621 gtaagtatga agcaaaaacg gcagctctga tacttgcttc acaggtcaac atttatttaa
     4681 caatcaagtg caattatatc tctcgccagc agaatgattt tcacgtttac tatgaaccac
     4741 ctttttattt ccgccgtgag ggattatgcg tttccgccag ttgttaccgc tttttggcgc
     4801 gctgtttgcg ttgtatatca tttggggctc aacctatttt gtcattcgga ttggcgtggg
     4861 aagctggcct ccgttaatga tggcgggcgt tcgattcctg gcagccggta ttttattgct
     4921 ggcatttttg ctactgcgcg gacacaaact tcccccgcta cgtccgctgc tcaatgccgc
     4981 gctgattggc ctgttattgc tggctgtcgg taatggcatg gtgacggttg ccgaacatca
     5041 aaatgttcct tccggcatcg ccgccgtagt gattgcaacc gtgcccctct ttaccctgtg
     5101 cttcagtcgc ctgtttggca ttaaaacgcg caaactggaa tgggtgggta ttgccattgg
     5161 gcttgccgga atcatcatgc tcaatagcgg tggaaattta agcggcaatc cgtggggcgc
     5221 gattctgatt ttaatcggct cgattagctg ggcgtttggc tcagtttatg gctcgcgcat
     5281 taccttacct gtagggatga tggcgggtgc gattgagatg ctggcggcag gcgtggtgtt
     5341 aatgatcgca tcgatgattg cgggtgaaaa actgacggcg ctcccttccc tgtcaggctt
     5401 ccttgcggtc ggctatctgg cgctgtttgg ttcgattatc gccattaacg cttatatgta
     5461 tttaatccgt aatgtcagtc cggctctcgc caccagctac gcctacgtta acccggtggt
     5521 cgcggtcttg ctgggtacgg gactgggtgg cgaaacgctg tcgaagattg aatggctggc
     5581 gctcggcgta attgttttcg cggtggtact ggtcacgttg ggaaaatatc tcttcccggc
     5641 caaacctgtt gtcgcgccag ttattcagga tgcatcaagc gagtaaatga atcccctgcg
     5701 tgtcgatctg cgcgctggca ccttcgccgc agatccactc ttccagacgc tcggtaagcg
     5761 ccgcatccgt cagcttctcg cgcctacgta acgcacactc ccagacaatc agtacgcgcc
     5821 agccgagctc ctgcaagcga ctgatatcgc ggcgatcgcg ttcaacattt ttacctatct
     5881 tctccagcca gaactcagtt cgagtcgcag gcactttaaa cagatagcag tgatgatgat
     5941 gccagaagca gccgtgggta aatatcacgc agcgatattc atcaacgaca aaatccgggc
     6001 gtccgggcag actggcgtcc tgaacgcgaa acgcaaggcc ctgcccggtt aacaaactgg
     6061 cgaggcgctt ctcaatcgcc gtatcacgcg tggcaatcgc gcgcatattt ttgctgcggg
     6121 tggccttatc gtgaacgtcg accatgttgt gcctcttgct gacgcaacgc caccgcctgt
     6181 ttgatttttg gctcaagcag ttttgccacc gcggcaaaga ccggcacgac caccgagttg
     6241 ccgaactggc gataggcctg agtgtccgaa accggaatgc ggaatttcgc ttctcccggc
     6301 gcttcaaaac ccattaagcg cgcgcattca cgaggcgtta accgacgtgg acgatgttgc
     6361 tgattcagcg gatcgtcaaa gtctttctca cccgtggcca tatcccaacc gcgatcgatt
     6421 aaaatttccg cgccatcttt gtaataacga gcagacagcg tacgggtgac gctttgcgga
     6481 ttgttcggat aaaccattcc ataaccgaag ccgttaccgc gcgcctgatg ttttttcgca
     6541 tatcgataga ggtacttcca cagcaccggc gtcaggatat atttcgcctc aaccatcggg
     6601 tccagcagct gcgccagcgt cactcgctgt gcagggaaac attcgctgat atcacgcaga
     6661 gtaaaatcgg ctttcagatt cagatcgcga cgaaaaccca ccagcacgat gcgttcacgg
     6721 tgctgcggca ggaaatgttt accatcgatg attttcggat cgtccggccc gttatcttct
     6781 gcatcggcaa cgtcatagcc cagttcgtcc agcgtctgca tgatgatgcg gaacgttttt
     6841 cccttatcgt gactcttcag gtttttgacg ttttcgagca caaacatcgc cggacgacgc
     6901 gcgtcgataa tgcgcaccac atcaaagaac agcgtgccct gggtatcgca ggcaaaaccg
     6961 tgcgcccgcc cgagcgagtt ctttttcgat acgccagcca gcgaaaatgg ctgacaaggg
     7021 aaaccggcca gtaaaacatc gtgttcagga atgtgttgac gaatatgttc cgccgccgcc
     7081 tcatcactca cgccttcttg atgactgagg gtgatgtcgc ggatatcttc attaaaatga
     7141 tgcgtcgccg ggtcgcaata atggttggct ttataagtgc gtaccgcatg tttgttccat
     7201 tcgctggtaa acacgcactg tccaccaatc gattcaaaac cacgacggat gccgccaatt
     7261 ccggcaaata gatcgataaa gcgaaacgca taatgcggat gatgttccgg tggtttgggt
     7321 aacaacgttt gcagatgggc aaactctttc tcacttaaac ggtgccatgc cgagtcattc
     7381 gccagcgcac gttttaaaat tgccgcgctc cagtgattct cacccacacc attaagctgc
     7441 gccaccagca ttttaacatc ataaatttgc agcagtttct ccagcattgc ctgtgcggca
     7501 ttcccggtgc tgtatgaatc ggttactgat atattttcct gcatagattt aaccggccat
     7561 ctgagatggc cgacaggtta acataattac tgccgtttag ggccacgcgc tcagccagcg
     7621 ggtgaaaacg catctatcac cttgtgatcg ataccttcat tctcccccgc cagttcggca
     7681 ccgagcttcg ccataaactc gactaaaaat tgcgcgttgt gctgtgccag ctgcttgcct
     7741 cttgcggttt gcatggtttg cggtaatttc agtaactttg tctggaaatg atccagcgcg
     7801 tagcgtttat catcaagcgg gcgatgctgt gcgaacggat cttcgccatc aaacagcgcc
     7861 aagcccaatg cccctgaaac ggcaaatacg cgcgccaggc caatcgcccc cagggcttcc
     7921 agccgatccg catcctagac gattttagct tccgttgtta agggggcgat ttgcgcgctg
     7981 aaactgtgag cggcaatggc atgacaaacg gcctcaattt tctccgccgg aaattgcacg
     8041 aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga acgctgccgt
     8101 tgcggatgat ttttcgccag gctcacgata tcgtgaaaat aacaggcggt taaaatcacc
     8161 agcatatcga catcgtcatc tgccgccagt ttctgtgccg tagcccagac acggcgaaaa
     8221 tgacacaggt catgcgccgc gtcctgatgt tgatgatggt ttttcaacca gttctcgaac
     8281 tgtgcctgcc agtgttgtaa ctccatacgc gctccgttgc caaagagtcg caaccttagc
     8341 agtttttatt catagcgtct gcaacgtgcg ggacgaggtc gggtgtacca tgcaatgccg
     8401 cctaacaagg caccaaccag ggccataaac agaaatccga ccaatgctcc taaccacttc
     8461 cccgcggtag atcctaaatc actttcaaat cccgacacgg gggcatcggg tatccggctc
     8521 atcacccaga tgtatcgcgc cattgcccag acgaaatagc cacaccatgt atagaaagcc
     8581 cac
//
LOCUS       DBKEKI010000161         8560 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.162, whole genome shotgun sequence.
ACCESSION   DBKEKI010000161 DBKEKI010000000
VERSION     DBKEKI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.162"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..3282)
                     /gene="nifJ"
                     /locus_tag="ACUQPX_003671"
                     /pseudo
     CDS             complement(1..3282)
                     /gene="nifJ"
                     /locus_tag="ACUQPX_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
     gene            3556..3822
                     /locus_tag="ACUQPX_003672"
     CDS             3556..3822
                     /locus_tag="ACUQPX_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hemolysin"
                     /protein_id="HHV9058215.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            complement(3819..4241)
                     /gene="hslJ"
                     /locus_tag="ACUQPX_003673"
     CDS             complement(3819..4241)
                     /gene="hslJ"
                     /locus_tag="ACUQPX_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HHV9058216.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4352..5341)
                     /gene="ldhA"
                     /locus_tag="ACUQPX_003674"
     CDS             complement(4352..5341)
                     /gene="ldhA"
                     /locus_tag="ACUQPX_003674"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HHV9058217.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5549..8195
                     /locus_tag="ACUQPX_003675"
                     /pseudo
     CDS             5549..8195
                     /locus_tag="ACUQPX_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
     gene            8192..8377
                     /locus_tag="ACUQPX_003676"
     CDS             8192..8377
                     /locus_tag="ACUQPX_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HHV9058218.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            8382..>8560
                     /locus_tag="ACUQPX_003677"
     CDS             8382..>8560
                     /locus_tag="ACUQPX_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HHV9058219.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINA"
BASE COUNT         2075 a         2122 c         2257 g         2106 t
ORIGIN      
        1 ccagaagccg gtagcagtga gttggcgcat ctggtcgtgg ctgagagcca gatcgtaacc
       61 atgctcttca cacgggctat aagcaatgat cagcgatggt cccggatacg cttccgcttc
      121 ctgaatcgct ttcaccgtct ggttcagctg cgcgccgaga gaaatctgcg ccacataaac
      181 atgaccgtac atcatcatac tgacgccaag atctttacgc gctttacgtt tgccgtgctc
      241 gccgaactta gttactgcac ccagcggtgt cgctttcgac gcctgaccac cggtgttgga
      301 atagcattgc gtatccagca ccagaatgtt gacgttttcc gtcaaactca atacatgatc
      361 cagaccgcca aagccgatat cgtaagccca gccgtcgcca ccaatcagcc agattgattt
      421 ttccaccagt gcatctgcgt cacgcagcag ttcatgtgct tcggcaacat cgttgagttg
      481 ctggcgtaaa gctgcgacct gttctcgacg aacctctggc gtagcgtctg atttcaacgc
      541 cgtcagtaat tccgccggga ttttatcggc aaattgatcc tgcagacgca gcacgcggac
      601 acggtgttga tcgaccgtca ggcggaaacc aaggccaaat tcggcattat cttcaaatag
      661 agagttcgcc catgccggcc cacgaccgtt ggcatcggtg gtatacggtg tagagggcag
      721 gttaccgcca taaattgaag aacagccggt ggcgttagcg atcaacatcc ggtcaccata
      781 gagctgagtc agcaatttaa tatacggcgt ctcgccacaa ccggagcaag cacctgaata
      841 ttcaaacagc ggtgtaatca gctgcgatgt acgaatatca atacgttcca gtttgctacg
      901 gtcgatttct ggcaggttga ggaagaaatc gtaattgatt ttctcttctt cgacatgttc
      961 caggcgagac atcatattga tggctttaat ctctggattc tgacggtctt tcgccgggca
     1021 aacttcgacg cacaggttac aaccggtgca atcttccggt gccacctgca agacatattt
     1081 ctgcccgcgc atatcacgcg atttcacatc tagcgaatgc aggctggcag gggcgttttc
     1141 catcgcttca ggcggtacca cttttgcgcg aatagctgag tgtgggcaag cggcgacgca
     1201 gtggttacat tgggtacaga gttcctcttt ccagatgggg atctcttcgg cgatattgcg
     1261 tttttcccag cgagtggtgc ccatcggcca ggtgccgtct ggcggcagcg ccgaaacggg
     1321 gagggcatca ccaagcccgg cgagcatcgc cgcggttacg gttttcacga aatcaggggc
     1381 ggcatcggaa accactggcg gtcgattggc gctgtgcgga tttaccggtt gcaacggaac
     1441 ttcttctacg gattcacgcg ccagcgccag agcctgccag ttgcgctcca ccagatcctg
     1501 acctttgcta ctgtaacttt tggcaatcgc accctgcaat tctgcgaggg cgctatcgcc
     1561 aggcagaatt tgcgtcagat ggaaaaaagc catctgcatg acggtattaa tacgggccgc
     1621 caggccacat tcgcgggcga ttttcgccgc gttaatcaca tagaagcgcg cttttttctg
     1681 gtttaacacg gcctgaactt cctgcggcaa gcgcgaccac acttcatctg cgctgtacgg
     1741 cgtattaagc aggaaaatgc cgccaggttt taaacgctca gccatctgat atttatcgat
     1801 aaactgcaac tggtggcagc caacaaaatc agcctaggaa atgagataag cggaacgaat
     1861 cggctgttcg ctcacgcgaa ggtgagaaac cgtcaggccg cccgcttttt tggagtcgta
     1921 aacaaaatag ccctgtgcgt accacggcgt ggaattaccg ataatcttga tattgttttt
     1981 ggttgcggaa acgctgccat cactaccaag gccataaaac aaggcttcaa gtttcgccga
     2041 gtttggcaga gtgttttccg gcaacggcag tgacagattg gtcacatcat cgtaaatacc
     2101 aaccgtaaag cgtgctttag gtttagccgc gttgagctcg gcaaataccg ccagtacaca
     2161 gtccgggcca aattctttgg atgaaagacc atagcgccca ccaatgacac ggggcagggt
     2221 ttcgcgctca ccattattaa aggcttctgc cagtgcggtc atcacatcca gatagagcgg
     2281 ttctgcctgg gcaccgggtt ctttggttct gtccagtacc gccacgctgc gtacggatcc
     2341 cggcagagct tgcagcaagt gtttggcgga gaagggacgg tacagacgaa ctttcagcac
     2401 gccaactttt tcgccacggg ttagtaattc atcaaccact tcttcacagg tgccaatggc
     2461 agagcccatc aggataatca cccgttccgc ttgcgggtgc ccgtaatact caaacggctg
     2521 atactgacga cctgtcgcgg cagcgaaatc attcatcgcc tgttcaacat ggtcatagac
     2581 cgcgttgtac caggggttgg tggcttcgcg agactggaaa taagtgtcag gattggcgga
     2641 cgtaccgcgg atcaccggat gttccgggtt gagcgcccgg gcgcgatgag catcaatttc
     2701 ggcctgcggc atgagatcaa gaatagtgtc atcggccagc gggacaattt tattgatttc
     2761 gtgggacgtg cggaaaccat caaagaaatg aataaatggc acgcggctct tcagcgttgc
     2821 gatgtgcgaa atgagagcaa agtcttgcgc ttcctggacg ttagccgcac acaacatcgc
     2881 gcaacccgtc tggcgcaccg ccataacgtc ggaatgatcg ccaaaaatag aaagtgcatg
     2941 tgtggcaacg gtacgtgccg ctacatgcag gacaaatggc gtcagttcgc ctgccagttt
     3001 gtacatcgtc gggatcatca gcagcaaacc ctgcgatgac gtaaacgatg ttgaaagggc
     3061 acccgtctgc aaagcgccat gcacggtaac gatagcaccc gcttccgact gcatttcaac
     3121 cacgcgtggt gtgtctcccc aaacgttctt caagccgttt ccggcccagg catcagcctg
     3181 ttctgccatc gtggaactgg gggtaatagg gtagatggcg ataacttcac tggtgcgaaa
     3241 cgccaccgaa gcaaccgcgc cattaccgtc aatagtaatc atatgacacc cttacattgc
     3301 gcaaatgagg ggcgcacgaa attgctgcgc gcccagtagt aatccttcaa ttttagcaaa
     3361 tggctttctt ctgcattttc gcttttgtgt cccccacatc agagtaatga atgttttgat
     3421 caaacagagg gcaaaaaaat agccataaaa aagtaaaaat gcggggcaac gcgcaggaca
     3481 gctctcgacg ccgccctctg tgctgcctat tatttatggc ggtgtcgttt ggcttgaaga
     3541 tcagaggaag aagagatgcg agcagcgttt tgggtagggt gtgccgcttt attgttgtcg
     3601 gcgtgtagta gtgagcctgt tcagcaggcg actgcggcgc acgtagcgcc aggtttaaaa
     3661 gcgtcgatgt ccagtagtgg agaagcaaat tgtgcaatga tcggcggttc gctttctgtt
     3721 gcccgtcaac tggatggtac ggcgattggg atgtgtgcat tacccaacgg caaacgctgt
     3781 agcgaacagt cacttgccgc cgggagctgt ggcagctatt aattcattaa atccgccagc
     3841 ttataagtta atgtctgttt cgcggtcgcc agcgttaact ggttcgcggt cagatccact
     3901 tgtgcacctt ctttcagcat ttcgctaatg gtgttatcga gttcattaag ctgcgggtta
     3961 gcgcacatca tacgggtcat tgccagccct ttggctgtca gttcaccatt agacagtttg
     4021 ccttcaccgc taaagcggtt acacatgctg ccggaaatca tcattttttc accaaagctg
     4081 atttctggcg gatttttatc gctggtcacg ggcttaccgt ttacgctttc cagcacaaag
     4141 cgatgatgct gtagctgttc tggcgtaaca gcaattttgt cattacttac acatcccgcc
     4201 atcagcaggc ttagcgcaac aaacgcggct actttcttca ttgtggttct caattacagt
     4261 ttctgactca ggactatttt aagaatagag aatgaaaggt cattggggat tatctgaatc
     4321 agctcccctg gattgcaggg gagcggcaag attaaaccag ttcgttcggg caggtttcac
     4381 ctttttccag attgcttaag ttttgcagcg tagtctgaga aatactggtc agagcttctg
     4441 ctgtcaggaa tgcctggtgc ccggtaaaca gcacgttgtg gcaggcagac aggcgacgga
     4501 atacgtcatc ctggatcacg tcgttggatt tatcttcaaa gaacagatcg cgttcgttct
     4561 catagacgtc catacccaac gaaccaattt tctgattttt cagcgcttca attgccgcct
     4621 gagaatcaat caatgcaccg cgactggtat tgacgatcat cacgccattt ttcatctgat
     4681 cgaaggcggc ttcgttcaac agatggtagt tttccggtgt cagcgggcag tgcagagaga
     4741 taacgtctga ttcagagaac agggttggca gatcgacata ctccacaccg agttccagcg
     4801 ccgctgcact tggatacgga tcgaacgcca gcagacgcat accaaaacct ttcagaatgc
     4861 gcagcatcgc cacaccgatt ttaccggtac cgataacgcc tgccgttttg ccatacatag
     4921 taaagccggt cagaccttcc agagagaagt tagcgtcacg ggtacgctga tacgcgcggt
     4981 gaatacggcg gttcagcgtc atcatcatac cgatggcgtg ttcagcaacg gcctctggat
     5041 cataggctgg aacacggact actttcagcc ccagttcttt tgccgcatca aggtcgacgt
     5101 tattgaaacc ggcacagcgc agggcgatat atttaacgcc gtgctttttc agctcttcca
     5161 gcaccgggcg gctgccgtca tcgtttacga aaatacatac cgcttcgcag ccattggcag
     5221 ttttagcggt tttttccgtc agcagaaagt caaaaaattc cagctcaaag ccaaaggact
     5281 cgttcacctg ttgcaggtac ttcttgtcgt actgttttgt gctataaacg gcgagtttca
     5341 taagactttc tccagtgatg ttgaatcaca tttaagctac taaaaatatt ttacaaaatt
     5401 tcaaatataa ttgaaagcca tggcgatatt gaaaaattca tcaacaacta tgcttagtgt
     5461 aggcgcaacc ttcaactgaa cggttaaaca tgccacaata cccgtattga atgcttaatt
     5521 tttcgctaaa tcaggatgtt aactacccat gctgggtaaa tataaagcct ttctcgcgct
     5581 gttattactg attattcttg tgccgttgac gctgctgatg acgctcgggc tgtgggttcc
     5641 cacgctggcg ggcatctggc taccgctcgg gacacgtatt gcattagatg aaagcccacg
     5701 cattacgcgt aaaggtttaa tcattcccga tctccgttat ctggtgggag attgtcagct
     5761 tgcgcatatc accaacgcca gcctttcaca tcccagccgc tggttattga acgtcggcac
     5821 ggtagaactt gattctgctt gtctggcgaa attgccgcag acggagcaat cgccagctgc
     5881 tccaaaaacc ctcgcgcagt ggcagtccat gctgcctaac acctggatca atatcgataa
     5941 actgattttt tctccctggc aggaatggca gggaaaactc tctctcgcat taacctctga
     6001 tatccagcaa ctgcgttatc agggcgaaaa agttaaattt caaggccagc tgaaagggca
     6061 acaacttaca gtcagcgaac tggatgtcgt cgcgtttgaa aatcagccgc cggttaaact
     6121 ggtgggggaa tttactatgc cgctcgtgcc ggatggactt cctgtaagtg ggcatgctac
     6181 tgcgacgtta aacttgccgc aggaaccgtc actggtggat gccgagctgg actggcagga
     6241 aaatagcggg caattgatcg tgctggcacg ggataacggc gatccgttgc tcgatttgcc
     6301 gtggcaaatt actcgtcaac aattgaccgt aagcgatggt cgctggagct ggctgtatgc
     6361 aggttttcct ttgagtggcc gactgggtgt caaagtcgac aactggcagg cagggcttga
     6421 gaacgctctg gtcagcggac gactgagtgt gctgacctag gggcaagcgg gtaagggcaa
     6481 cgcggtgctt aattttggcc caggaaaatt aagcatggat aacagtcaac tgcctctgca
     6541 gctgaccggt gaagcgaaac aggcggacct cattttatat gcccgtttac ctgcgcagct
     6601 aagtggaagt ctgtctgacc caacgctgac ctttgagcca ggcgcgttac ttcgttcaaa
     6661 gggaagagtc atcgattcgc tggacatcga tgaaatccgc tggcctttag cgggtgtaaa
     6721 agtcacccaa cgtggtgttg acggacgttt gcaggccatt ttgcaggcgc atgaaaatga
     6781 actgggcgat ttcgtgctgc atatggatgg gctggcgaat gattttctcc ctgacgctgg
     6841 acgctggccg ctggcagtgg cgctactggg gaaaagggag ttttacaccg atgaatgcca
     6901 cctgggatgt cgcaggaaaa ggtgagtggc atgacagcac gattacgctg accgatctct
     6961 ccaccggttt cgaccagtta caatacggta cgatgacggt agaaaagccg cgattaattc
     7021 tcgacaagcc cgtcgtctgg gtacgtgacg cacagcatcc ctccttcagc ggcgcgctgt
     7081 cactggacgc cgggcaaacg ctgttcactg gcggcagtgt gttaccgcca tcaaccttaa
     7141 aatttagcgt cgatgggcgc gatccgacct atttcctctt taaaggcgat ttacatgctg
     7201 gtgagattgg cccggttcgg gtaaatggtc gctgggacgg tattcgtctg cgcggtaacg
     7261 cctggtggcc aaaacaatca ctgactgtat tccagccgct ggtgccaccc gactggaaga
     7321 tgaacttacg cgatggtgaa ctgtatgctc aggttgcatt ttctgctgcg cctgaacaag
     7381 gattccgcgc gggagggcac ggcgtgttga aaggcggtag tgcctggatg ccagataatc
     7441 aggttaacgg tgtcgatttt gtcctgcctt tccgttttgc cgatggagcc tggcatctgg
     7501 ggactcgcgg ccccgttacg ttgcgaattg ccgaagtgat taatctggtg acagcgaaaa
     7561 atattactgc tgatttgcaa gggcgttatc cgtggactga agaagaaccc ttgctgttga
     7621 ctgatgttag cgtcgatgtg ttaggcggta acgtgctgat gaaacaatta cgtatgccgc
     7681 aacatgaccc ggcgctgttg cggctgaata atctctcatc cagcgaactg gttagcgccg
     7741 tcaatccgaa acaattcgcc atgtccgggg catttagtgg tgcattgccg ttatggctaa
     7801 acaacgaaaa atggatagtg aaagatggtt ggctggcaaa tagcgggcca atgaccttgc
     7861 ggctggataa agataccgca gatgcggtgg tgaaagacaa tatgactgcg ggttcagcaa
     7921 ttaactggtt gcgctatatg gaaattagcc ggtcatcgac aaaaattaac ttagataatc
     7981 tcggtttatt aaccatgcag gccaacatta caggtaccag tcgcgttgat ggtaaaagcg
     8041 gtacggtaaa ccttaattac catcatgaag agaatatttt tacgctgtgg cgcagtttac
     8101 gctttggcga taatctccag gcatggttgg agcagaacgc acgtctgccg ggaaatgact
     8161 gtccgcaagg aaaagagtgt gaggaaaaac aatgaaaatt ttactggctg cgttgacgtc
     8221 atcttttatg ctggttggct gtacgcctcg cattgaagtc gctgcaccta aggaaccgat
     8281 cactatcaat atgaacgtta aaattgagca tgagatcatc atcaaggcag acaaagatgt
     8341 cgaagagctg cttgaaactc gtagcgatct tttctgaggc gatgatgaag aaaacattac
     8401 ttctttgtgc gtttcttgtt gggctggtaa gcagcaatgt aatggcattg actctggatg
     8461 aagccagaac tcaggggcgg gtaggtgaaa cattttatgg ttatctggtt gcgctgaaaa
     8521 cggatgctga aacagagaaa ttagtaaccg aaattaatgc
//
LOCUS       DBKEKI010000162         8529 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.163, whole genome shotgun sequence.
ACCESSION   DBKEKI010000162 DBKEKI010000000
VERSION     DBKEKI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.163"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..473
                     /locus_tag="ACUQPX_003678"
     CDS             <1..473
                     /locus_tag="ACUQPX_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHV9058220.1"
                     /translation="GGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKTGET
                     ATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPEPVAK
                     VCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRYMPA"
     gene            1348..1482
                     /locus_tag="ACUQPX_003679"
     CDS             1348..1482
                     /locus_tag="ACUQPX_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HHV9058221.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(1727..2836)
                     /gene="wzz(fepE)"
                     /locus_tag="ACUQPX_003680"
     CDS             complement(1727..2836)
                     /gene="wzz(fepE)"
                     /locus_tag="ACUQPX_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HHV9058222.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(3336..3635)
                     /locus_tag="ACUQPX_003681"
     CDS             complement(3336..3635)
                     /locus_tag="ACUQPX_003681"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058223.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYG
                     LNISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFM"
     gene            complement(4019..4168)
                     /locus_tag="ACUQPX_003682"
     CDS             complement(4019..4168)
                     /locus_tag="ACUQPX_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HHV9058224.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMMQVTEENLHAPVDFGPPV
                     GREAL"
     gene            complement(4413..5104)
                     /locus_tag="ACUQPX_003683"
     CDS             complement(join(4413..4855,4855..5104))
                     /locus_tag="ACUQPX_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HHV9058225.1"
                     /translation="MASISISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARFIGVGLNTIFRHFKKLRPQSVTSRIQPGRDV
                     IVCAEMDEQWSYVGAKSRQRWLFYAYDRVRRTVVAHVFGERTMFTLERLLSLLSAFDV
                     VVLMTDGWPLYESRLKEKLHVISKRYTQRIERHNLRQHLARPGRKSLSFSKSVELHDK
                     VIGHYLNIKHYQ"
     gene            5395..6915
                     /locus_tag="ACUQPX_003684"
     CDS             5395..6915
                     /locus_tag="ACUQPX_003684"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HHV9058226.1"
                     /translation="MFLISIIQMFSVMIIMLNIKIKDAFRAIILLLLLLLSMVISIEI
                     SSIISIGEYIPTLALANISETNSVGWKIKLLPFLVFLSFMIASLILKRLIKEKKIRNI
                     MVVVFLLSLLIPISPINAFFSSSYEVYSEAKEYNMSPHEISDIKSKYTKETVTNDSYS
                     HEIINGNGKNILVIFIEGMSSLVISKELTPNLYEFRKKSISFNNYYNHTAATFRGLRG
                     QLTSSYQMTGGYHKDNSGLGQASKVEIEHKLNTYNYITKLPIILEENGYHTYFQASNS
                     IDDPLSLMLSTLKFNHIYGREDYNKKSGELTDKESFDLLFKKLNEAKQPFFYGVYTVG
                     THLGLDSPDVKFYDGRNEYLNHFHQLDHWFGDFISHFNKTSLSKDTIVIITSDHASYP
                     SEDYKKTFKTDNTFFVDQIPLIIWSNGVEFKDINAHGLNSLSLTPTILDILGIKESHN
                     MFIGESLFEKEKNKNELNCTSAIGDLYVKTCNGKMEIEKRKEKTELIKQLQLFGDR"
     gene            complement(6984..7736)
                     /locus_tag="ACUQPX_003685"
                     /pseudo
     CDS             complement(6984..7736)
                     /locus_tag="ACUQPX_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009652796.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            7787..8059
                     /locus_tag="ACUQPX_003686"
     CDS             7787..8059
                     /locus_tag="ACUQPX_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058227.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMHPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            8040..>8529
                     /locus_tag="ACUQPX_003687"
     CDS             8040..>8529
                     /locus_tag="ACUQPX_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISSbo1 family transposase"
                     /protein_id="HHV9058228.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYNCDKPDCPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLN
                     WHP"
BASE COUNT         2472 a         1933 c         1854 g         2270 t
ORIGIN      
        1 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
       61 cctcccggct ggcacattac aacggagggg cgagcctgag cttccgttac ctggaccaca
      121 aaacgggaga aacggcgacg gaaacgctga cacagcgtga gctggtcgcg aggctgaaac
      181 agcacatccc ggagaagttt tttaagatgg tgaggtactt cgggttcctt gccaaccggg
      241 tgtgtggaga gaagctgccg caggtgtacc gtgctctggg gatggataaa ccggaaccag
      301 tggcgaaggt gtgctatgca caaatggtga agcagttcct gagtcgtgac ccgttcgaat
      361 gcgtgctgtg tggcggccgg atggtatacc gccgggccat tgcggggctg aatgtggagg
      421 ggctgaagaa aaacgcgcgg gatatcagtc tgctgaggta tatgccggcc tgacacaggc
      481 taagtgtgcc cgggaatcgg tgcaagtata aaaaacagcc tgttgcggta taagaatcga
      541 ccatgcagag agagaaaaca ccggagtgga gggaaaagca gaaaagctca cgtggcataa
      601 ggcgtggaca gcggtatcgt ctggtttttc aatttcctat acgtgaactc cagaacagca
      661 tccagattcg ctttgaggcc gaaagtgaaa caggaacata acgaagctga cggaggcggt
      721 cacggctttc ccgtgggcgt tttcgtcagc agacaagccc gtcagggcgc gtcagggggt
      781 tttagcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
      841 ggcttaatta tgcggcggca gagcagatgg tgagagaaca gcgacatatg aggtgtgaaa
      901 tgctgcacag atacgtaagg agaaaatgca gcttccggcg ctcttccgct tcctcgctca
      961 ctgactcgct acgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaagacgg
     1021 taatacggtt atccacagaa tcaggggata aggccggaaa gaacatgtga gcaaaaaggc
     1081 aaagggcaac gaaaggctgc gccgctggcg tttttccata ggctccgccc ccctgacgag
     1141 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact taaagatacc
     1201 aggcgtttcc ccctggtggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
     1261 gtgtcattcc gctgttatgg ccgcgttcgt ctcattccac gcctgacact cagttccggg
     1321 taggcagttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1381 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt aacgccattg
     1441 gcagcggcca ctggtaaaca ggatgtgcga gagatgtatt gagagttctt gaagtggcag
     1501 ccttactgag gctacactag aaggacagat ttggtgacat gtctcgcaca agactgttac
     1561 cacggttcag aagttcccca acttactgaa ccttcgaaaa accacctccc caggtggttt
     1621 tttcgttttc tagagtagaa gactacggct gtaagggaat gatctcaaga agatcctttg
     1681 aactttttat aaaagttgct tataaaatga tgcatcagca caacttttac tcaatcacac
     1741 tcatctttct ggcctccatg gcatgacgta aaagtatata gccacatgca agcatccctc
     1801 caatgacaga agaaagaatt acaattatgg cattacttgg cccatccttt ctgaccggta
     1861 acgttgggct caattgatac tgaaacggca taaaatcaac atcccccact ttcgccacaa
     1921 ccaattgatt cacaacatat tgacgatttc gcaactcccc actcaattcc gaaacatcct
     1981 tgattgattt tttgatgttc aatttagttg ctataccatc agaaccgaga gccacaggaa
     2041 aatctgggtc atccttcata atctgaccat tgctgtatac aggttttttt attccagccg
     2101 cattagcaac ctccagtgaa taattgaggc gtcgaatatc tgcattcagc tggtttctaa
     2161 tttttatctc atccagtgca agaatttctt tttcaacatt agttttaact tctagtttat
     2221 ttcttatatc ttccatcaaa tcccttacaa caagggaaga aacataatta atatacccag
     2281 acaacacatc gtgagcttct ttacttgttg gagcagtaaa actcagagtc catgatacat
     2341 acaatgatga cttgtcgttt ttcttactgg cattatcatc aatcgacttc atatttttac
     2401 tcaactcaac aattgcacga tgcagttcga gagcgctaat gttattcttt ttaataatct
     2461 ccataacata aggagatgat ctcatatact cattcaacga attaacggac tgaaatttct
     2521 taataaacag agcaaatgcg ctatcacgat tgatatcaaa atccactccc agaacatgaa
     2581 gcttggaaag ctccttattt aattcactcc attgaacaga ctcagcagga gtaaccacag
     2641 cagaactagt ccatttttgt ggcaaaacaa aagaaacaag aaagccaata caagcaaaaa
     2701 atagaatcac cccaacaatt tttatttttg ctgcccataa aacgctaatc aagccaaaaa
     2761 ggtcaatttc ttcattacca ttcggcaaca tgttgtaatt agaaaaatgt tcgtcagaga
     2821 cctttgagat tttcattgaa aaaaacacaa tatattaaca tatggttttt ttattctatc
     2881 atatgttagg aaaaaaccta aaaaccattc agcagacatt taacataaca ttttattgcg
     2941 tgcaccgcct cagaggctct cagggcattt ctgacgggaa accggcaaaa caggcaacaa
     3001 accacccgaa aacccgccag aaacgcgcag gctctgtttt tactgcatgc atgagtatgc
     3061 acgcaagtgc atagcgcgaa aattcacccc attcagccag ggtaagtcct gtttccggca
     3121 ttcttcccat ccgaaaacgg tgaacgggga cgcaatttat tgcgtggcag tgaggcattg
     3181 accaagccgt aaggcacggt aggtaattgc aggtatccga caatttcgag tagaggatcc
     3241 actatttcac tacttagaat tcaaaatcag aatcctttct tcctgctaag gctatctctg
     3301 aatgactcaa ccactctttg agattcttct tctgctcaca tgaagtcatt cccggacaga
     3361 aaaaaaccat tgtcaggccc actggcttca gagaaattcg tttctgcagc agacacagaa
     3421 tccacatcag caagagacgg ttcatctgta ctttttctca atctgtaaag agccttatca
     3481 aacgcaacca gtgagatatt caggccgtaa cgttcattca gcacagtcac aatctcttcc
     3541 ctacggatac catttttctt ccaggtttct atatcgttct tggaatccct gacagccgca
     3601 atggctgttc ttggaccagg gttaaaattc gccatatacc gcccataatg tcttaaattt
     3661 gtctcataat agtccaaaaa taagattgaa acgcaaaaaa tgtcttacaa ttatcttatt
     3721 gttgtccttt ccggttcatg tatccatgaa ccgcctgatg tggaacgctt tttatcgatc
     3781 tcatgcgcag ccaaagatag attcattctt taaaagtcag gttacattat cccttaaatg
     3841 gtcatacacc atacgcaacc gcgattttca tcgcaattgc atgaattaaa aggtaaaaac
     3901 tgaagtcccc gtttatttat gtaatacgca acgtattaca accacctgta attacatgaa
     3961 aataaagaaa taagatgtaa taccggcgta ttaccacgta cgcgggtaag cgaaaaagct
     4021 ataacgcttc ccggccgaca ggcggcccaa aatccaccgg ggcgtgcagg ttctcttcag
     4081 taacctgcat cattaaatca aaaaggtcat attcgccggg aagctgcgta tcagtcgaat
     4141 ccagttgttc atcatgttca gatggcatgg ggtgatatcc agtagctgag aggtggttag
     4201 cgggtttcgg gacgcagccc taaaccagtc acgtagcgct agcggagtgt atactgactt
     4261 aggcggtagt gtagtatgac agattctgtg gttacggcaa agtaacagcc ctgtctagga
     4321 gagggctgtt atctgatata ggcttgtttt tttctcaccg tttttgcgag cgctttttcc
     4381 tgccaagagg taggtagggt aatgaatcca acttactgat agtgttttat gtttagataa
     4441 tgcccgatga ctttgtcatg cagctccacc gattttgaga acgacagtga cttccgtccc
     4501 ggccttgcca gatgctgcct cagattatgc cgctcaattc gctgcgtata tcgcttgctg
     4561 ataacgtgca gcttttcctt caggcgggat tcataaagcg gccatccgtc cgtcatcaat
     4621 accaccacgt caaaggccga cagcaggctc agaagacgct ccagcgtgaa cattgtgcgt
     4681 tcaccaaaaa cgtgggccac gacagtcctg cgtaccctgt catacgcgta aaacagccag
     4741 cgctggcgtg atttagcccc gacgtagctc cactgttcgt ccatttccgc gcagacgatg
     4801 acgtccctgc ccggctgtat gcgcgaggtt accgactgcg gcctgagttt tttaaatggc
     4861 ggaaaatcgt gttgaggcca acgccaataa agcgggcggt tgcccggcat ccgacgccat
     4921 tcatggccat atcaatgatt ttctggtgtg taccgggttg agaggcggtg taagtgaact
     4981 gtagctgcca tgttttacgg cagtgagagc agagatagcg ctgatgtccg gcagtacttt
     5041 taccgttacg cacaacgccg tcagtagcgg aacaggaggg acagctgatg gaaatggaag
     5101 ccaccggagc acctcaaaag caccgtcatc cactaaatca gtaagttgga agcatcaccg
     5161 cactagcaga cagttaatta aaaaagtata atccctctcc ggatttattg aataatggag
     5221 aattatgctg ttttctatgt cttatttagt caaatgcgct tatcaatatt gtgataagct
     5281 cccgagtcta aacttaactt actgatttta ggttgaggaa gaccggcatt tttgtagatc
     5341 cacggcataa tatctattta ttcaacaacg ccccccaaaa aaaggttttt ataaatgttt
     5401 ttaatttcaa tcattcaaat gttctcggtt atgattataa tgcttaatat aaaaataaaa
     5461 gatgcattta gagcaattat tctgctttta cttctattac tatccatggt tatatctatt
     5521 gaaataagca gcattatatc aataggtgag tacataccca cacttgcact agcaaatata
     5581 tcagagacaa atagcgttgg ctggaaaata aaactactac cttttcttgt gttcttgtca
     5641 tttatgattg catcattgat tttgaaaaga ttgataaaag aaaaaaaaat aagaaatata
     5701 atggttgttg tttttttatt atcattgtta ataccaatat ctccaataaa tgcttttttt
     5761 tcttcatcat atgaagttta ttcggaagca aaagaataca atatgtcacc acatgaaata
     5821 agtgatataa agtcaaaata cacaaaagaa acagtaacaa acgacagtta ctcacatgaa
     5881 ataataaatg gaaacggaaa aaatatttta gtaatattta ttgaaggtat gtcttcattg
     5941 gtaatatcta aggagctaac cccgaatcta tatgaattta gaaaaaaatc aatatcattt
     6001 aacaactatt acaaccatac cgcagctaca tttagaggtt taagaggaca gttaacctca
     6061 tcatatcaaa tgaccggtgg gtaccataaa gataattctg gccttggcca agcatctaaa
     6121 gtagagatcg aacataaact aaatacttac aattacataa caaaattacc aatcatactt
     6181 gaggaaaatg gttaccatac atattttcaa gcatctaatt caatagatga cccattgagt
     6241 ttaatgctat caactttaaa gtttaatcat atatacggaa gggaggacta taacaaaaag
     6301 tcaggtgaac tcacagacaa agagtctttc gatttattat ttaaaaaatt aaatgaggct
     6361 aaacaacctt tcttctatgg tgtttatact gtaggtacac accttggctt agatagccct
     6421 gatgtaaagt tttatgatgg gagaaatgaa tatttaaatc actttcatca attagaccat
     6481 tggtttggtg attttatatc tcattttaat aagacaagct tatcgaaaga tacaattgtc
     6541 ataatcacaa gtgaccatgc aagttaccca tcagaagatt acaaaaaaac attcaaaact
     6601 gataatactt tttttgttga tcagattcca ttaataatat ggagtaatgg tgttgagttc
     6661 aaagacatta atgcacacgg tttaaattca ctatctttaa cgccaacaat actagatatt
     6721 cttgggatca aggagtcaca caacatgttc ataggtgagt ctctatttga aaaagaaaaa
     6781 aataaaaatg aactcaactg tacatctgca ataggtgacc tgtatgtgaa aacgtgtaat
     6841 ggaaaaatgg aaatcgagaa aagaaaagaa aaaacagaat taataaaaca attgcaactt
     6901 tttggtgata gataattaaa aggcggtttt accccatgtt gattattagt ccacgtgggg
     6961 ttagtttaaa atgataataa aacttacttt ttttcattct tgagccgcct gtagaatgtg
     7021 gctcgactaa cacctaaaga ctcccattct ttttttaaag ttatatgaat taatattaaa
     7081 actaccttta ggccgtcctc ctgaattgct tagctttcct cctttacccc ctctctttct
     7141 ttgaatttct gaacgggcgt attttttaca tactcaataa aacgatcctc tgaaaaatta
     7201 ttcgctgtcc attttgagat gctttttgat attccaagaa cttcattttc acttaatggt
     7261 gatacgaatt gtaaattgta ggcacaggct cgttcatagc aggcttgtaa ccactgctca
     7321 tactcaggcc acccctgacg gatggcacga tatgcccact tgcgggtttt gtcgaacaga
     7381 gtgcagttac ggcctaaacc ataatcggcg gcaattttcc agtgactgtg gttcggattc
     7441 ttgcaaatta gccccgaata tcccgtgtca gctcccaatt ttctacgtag cgcattctct
     7501 acggcagctg cgtagcgtaa cggtttcatt tttccgtcag gtgctgtgcg tatagaggtt
     7561 tccagcgcat aaagcaggtg cgcgtgtccg ttttcggggt tggtgatggt cagcgtaggt
     7621 gcgggagcgt tgcggtcgct ccagtcaatg gctgcgccga tgcggtcaac atcaaatccc
     7681 agccagaaca tggcgtgagg ctgattgaac cacggcagcc tggcggctgc aaatcggggt
     7741 gtggcagaat atgagctttc agagtaacca tccagaatga gttcccatgt cacacaacct
     7801 ggagcaccag aaagtccaca ccagaatggt gaaagaggtt ctgaaagccg tcgccagagc
     7861 caataatcac ccttataagt ccgtatttgc agactttatc accggacatc cctcctgcac
     7921 ggtctgtttc tgggaaacct tccacaaaat gcacccggat tcaccgtacg aatacgtcac
     7981 gttctgccat acctgccgtc gttttgattt atacgaaaca gaagcggaga tgaaggcgga
     8041 tgacccgaag tggtggtgat tttcagcccc gaccgctgaa acgcctgttc acagccaacc
     8101 agtgctggac atccttcctg gatgcgggcg gtctgcgcga tatcgaggtt gaggctgtca
     8161 caaaaatgct ggcctgcggc acgcggatac tgggcgtgaa ggaatacaac tgcgataaac
     8221 ctgactgccc ccacgtcaga tacgtcacta actcatgcgg cagccgtgcc tgcccgtcct
     8281 gcggaaaaaa ggccacagac ctgtggatag cgacacagct gaatcgtctt cctgactgcg
     8341 actgggtaca cctggtcttc accctgccgg acacgctgtg gccggtgttc gaaagcaacc
     8401 ggtggctgct gaatgacgtg tgccgtctgg cggtggagaa tctgctgtat gccgcccgga
     8461 aacgggggca ggaacccggt atcttctgcg ccatccacac gtatggccgt cgtctcaact
     8521 ggcatccgc
//
LOCUS       DBKEKI010000163         7933 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.164, whole genome shotgun sequence.
ACCESSION   DBKEKI010000163 DBKEKI010000000
VERSION     DBKEKI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.164"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            249..1751
                     /gene="proP"
                     /locus_tag="ACUQPX_003688"
     CDS             249..1751
                     /gene="proP"
                     /locus_tag="ACUQPX_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HHV9058229.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            1863..1952
                     /gene="pmrR"
                     /locus_tag="ACUQPX_003689"
     CDS             1863..1952
                     /gene="pmrR"
                     /locus_tag="ACUQPX_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HHV9058230.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(1928..3028)
                     /gene="pmrB"
                     /locus_tag="ACUQPX_003690"
     CDS             complement(1928..3028)
                     /gene="pmrB"
                     /locus_tag="ACUQPX_003690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HHV9058231.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(3029..3697)
                     /gene="basR"
                     /locus_tag="ACUQPX_003691"
     CDS             complement(3029..3697)
                     /gene="basR"
                     /locus_tag="ACUQPX_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HHV9058232.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(3694..5337)
                     /gene="eptA"
                     /locus_tag="ACUQPX_003692"
     CDS             complement(3694..5337)
                     /gene="eptA"
                     /locus_tag="ACUQPX_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HHV9058233.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(5441..6778)
                     /gene="adiC"
                     /locus_tag="ACUQPX_003693"
     CDS             complement(5441..6778)
                     /gene="adiC"
                     /locus_tag="ACUQPX_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HHV9058234.1"
                     /translation="MSSDVDAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYTRRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDRLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(6915..7676)
                     /gene="adiY"
                     /locus_tag="ACUQPX_003694"
     CDS             complement(6915..7676)
                     /gene="adiY"
                     /locus_tag="ACUQPX_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HHV9058235.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
BASE COUNT         1974 a         1926 c         2008 g         2025 t
ORIGIN      
        1 aatgagtcct aaacgaaatc catgtgtgaa gttgatcaca aatttaaaca ctggtagggt
       61 aaaaaggtca ttaactgccc aattcaggcg tcaactggtt tgattgtaca ttccttaacc
      121 ggagggtgta agcaaacccg ctacgcttgt tacagagatt gcatcctgca attcccgctc
      181 cccttttgcg gccgtcgcgc tgatttttct ggcgtttgcg gaaatgggcc aactctgcga
      241 ggaaagctat gctgaaaagg aaaaaagtaa aaccgattac ccttcgtgat gtcaccatta
      301 ttgatgacgg taaactgcgt aaagccatta ccgcagcatc actgggtaat gcaatggaat
      361 ggtttgattt tggtgtttat ggttttgttg cttacgcatt aggtaaagtt tttttcccgg
      421 gggctgaccc cagcgtgcag atggttgctg cacttgccac tttctccgtt ccctttctga
      481 ttcgaccgct tggcggactc ttctttggta tgttgggcga taaatatggt cgccagaaga
      541 tcctcgctat cactattgtg attatgtcga tcagtacgtt ctgtattggc ttaataccgt
      601 cctacgacac gattggtatt tgggcaccga ttctgctgtt gatctgtaag atggcacaag
      661 gtttctcggt cggcggtgaa tatactgggg cgtcgatatt tgttgcggaa tactcccctg
      721 accgtaaacg tggctttatg ggcagctggc tggacttcgg ttctattgcc gggtttgtgc
      781 tgggtgcggg cgtggtggtg ttaatttcga ccattgtcgg cgaagcgaac ttcctcgact
      841 ggggctggcg tattccgttc tttattgctc tgccgttagg gattatcggg ctttacctgc
      901 gccatgcgct ggaagaaact ccggcgttcc agcagcatgt tgataaactg gaacagggcg
      961 accgcgaagg tttgcaggat ggcccgaaag tctcgtttaa agagattgcc actaaatact
     1021 ggcgcagcct gttgacatgt attggtctgg taattgccac caacgtgact tactacatgt
     1081 tgctgaccta tatgccgagt tatttgtcgc ataacctgca ttactccgaa gaccacgggg
     1141 tgctgattat tatcgccatt atgatcggta tgctgtttgt ccagccggtg atgggcttgc
     1201 tgagtgaccg ttttggccgt cgtccgtttg tgctacttgg tagtgttgca ctgtttgtgt
     1261 tggcgatccc ggcgtttatt ctgattaaca gtaacgtcat cggcctgatt tttgccgggt
     1321 tactgatgct ggcggtgatc cttaactgct ttatgggcgt tatggcttct accttgccag
     1381 cgatgttccc gacgcatatc cgttacagcg cgctggcggc ggcatttaat atttcggtgc
     1441 tggttgccgg tctgacgcca acactggcgg cctggctggt cgaaagctcg cagaatctga
     1501 tgatgcctgc ctattacctg atggtagtgg cggtgattgg tttaatcacc ggcgtaacca
     1561 tgaaagagac ggcaaatcgt ccgttgaaag gtgcgacacc ggcggcgtca gatatacagg
     1621 aagcgaagga aattctcgtc gagcattacg ataatatcga gcagaaaatc gataatattg
     1681 accacgagat tgccgatttg caggcgaaac gtacccgctt ggtgcagcaa catccgcgaa
     1741 ttgatgaata agctgaaacg gatggcctga tgtgacgctg tcttatcagg ccaattgaac
     1801 tcttaaggtt cacttaatct ctgacgcgca tactctcctc caggttaacg gaggagagtg
     1861 caatgaaaaa ccgtgtttat gaaagtttaa ctaccgtgtt cagcgtgctg gtggtcagca
     1921 gctttcttta tatctggttt gccacgttct gatctttctt cagccgtacc caggcccgcg
     1981 tgccggaagt ctcttgccgg ttttgcagga aaaactgccc gtgatgcaac tgtgtaatgc
     2041 ggctgacaat acttaacccc agaccaatcc cgccataacg gctgtccata cgtacaaacg
     2101 ctttactcaa ctccccgcat ttactctcat caatacctgg tccttcatct tcaactgcca
     2161 tgaccgctcc gccatcttct tgcagcttaa tcataatgtt gctgccttgc gggctgtaac
     2221 gatgggcgtt ttctaccaag tttcgcaata acatccgcag cagggttgca tcaccctgaa
     2281 cggtgatgtc ggcggcgctc tctggcaata gcagggtttg ctgtcgctgg tcgagcatgg
     2341 tactgagttc gtcatacgag gggagaatga catcttccag cagttttaca tgttgataat
     2401 taccggaaga aaatgactgt ccggcacgcg ccagttgcag cagctgggag acgctctcca
     2461 tcatctgatc aagccgtgcc actaacggtg ctacatcaat gtgatgcgtt ttcgccagca
     2521 gttccagatg caaacgcacc cccgccagtg gcgttcgcag ttcgtgcgcg acgtcagcgg
     2581 taaacaacct ttcgttatcc agcgtgctgg tcaggcgact gaccagatcg ttcaacgccg
     2641 aaaccaccgc ttcgatttcg agggtggcgc tgtgaatggc aatgggcgtt aagttgtcgg
     2701 cggtgcgcgc ttccagctct ttttgcagct ccgccagcgg gcgggtgatg cggcgtaccg
     2761 cctgatagca gataaatagc gtcaggctga ccataaagac gccggggaca atcaggctgg
     2821 cgaccgcctc gcggatctca cgcatgatgt ggcgatcgtt gttgcgattg tcgcgtagcg
     2881 cctgctcaaa cagctgaatc tgctcggtac tttcatgcca tagccagaag acgctgatca
     2941 gctcaaacac caacaaaatg gccccgatgg tcaatatcag ccgttggcgc agcgatattg
     3001 gtcggcgcag aaaacgcatc agattcaatt agttttcctc attcgcgacc agcatatagc
     3061 caaagccgcg cacggtgcgg atacgggctt tgcccacttt gtcgcgcaga ttgtggatat
     3121 gcacttccag ggtgttggtc gagggttcat tgtcccagtt atagatgtcg ttgtagagaa
     3181 tttcccgatg taccggactg cctgctttga gcattaaccg tgacagcaga gcatattctt
     3241 tgggcgtcag aatcaactct tcaccgccca tccatacctg acggcgaccc atattcagcg
     3301 tcagattgcc aacaatcagc tcactttcgc cctgattatt atggcgtcgt agcagggcgc
     3361 ggatacgggc gtgtaactct tccagcgcaa aaggcttcac cagatagtca tcggcaccga
     3421 catccagccc ggcgattttg tcggtcagcg tatcgcgagc ggtgaggatc agtaccggca
     3481 gggtgtattt tttctgccgg atacgggcga gaaaatgcag tccatcttcg tcgggtaacc
     3541 ctaaatccag taccaccagg ctgtaatgac cgtcctcaag gctttgttcc gccatccgcg
     3601 cggttgtcac gccatcgcac gcgtagcctt cggtttgcgc cgccagaatc agtccctgca
     3661 ataacagcgt atcgtcttca acaatcagaa ttttcattta ctcactctcc tgcaagtttg
     3721 cagaatatca tccgcggcct ggtaatactt cgtctcaacg ccagttaatc ccaatagcgt
     3781 ggagaataaa ttgtcttgtg aatagtgttg cgtttgcgcc tgtttttgca ggcagttctg
     3841 gtcaacctga taccgctttt gataatcctc cgacagccac agcagcattg gcacctgttt
     3901 ttggctatcc ggggcgatgg cataaggcag accgtgcaga tagatgccat tttcacctaa
     3961 cgattcaccg tggtcagaaa gataaaccag gctggtggta aatttatcct gatgttcttt
     4021 aagcagatta atcgctttat caacaatata gtcgacgtaa accagcgtgt tgtcgtaagt
     4081 gttcaccagt tgctctttgg tacaggtctg gatctcattg gtgtcgcagg ttggggtaaa
     4141 tttcctgaac tgcggcggat agcggttgta ataggtcgga ccgtggctgc cgatggtgtg
     4201 taagacaatc acgccatcac cctgcaggtt attgatgtac tcttccagcc cgtggaacag
     4261 cacttcgtca tagcattcgc cgttgatgca ctgatcaggc agattcagcg cggtgacgtt
     4321 ctggtgaggc acgcggtcgc aggcaccttt acagccgcca tcgttgtcat tccacagcac
     4381 gttgatgccc gctcgctgaa tgatatccag cacgccttcc tggtgctgtg ccagctcttc
     4441 tttgtagtgc tcacgcggca tatccgagaa catgcacggt actgaaactg ccgttgccgt
     4501 gccgcaagat gcggtattag ggaaatagac cacgttatct ttcgccagcc gcgggttagt
     4561 ttcacgcggg tagccgttga gggagaagtt ctccgcccgc gaggtttcgc cgacaatcag
     4621 gatggtcaaa tttttacgtt tttcgttctg cattaacggg ttgcggtgcg cgtcttcacc
     4681 aattcgcacc agcggcagat ttgccagtcg ctgatgggag taccatgacc agctggcaac
     4741 aatgctgtta gaggggctta aggatttcac cagctctttg ttattgcgga acaacgaggc
     4801 gtagtcttta taaaacagtg cggcgaccag caaaatcagc agtacagaaa ccagaatatt
     4861 ggctccacgg aaaagaacac tgcgcagacg cgaggtggca ggtttgattt ttagccagca
     4921 ggcaatcagc gcagcaagca cgccgctgaa tcccagcgtt aataacattt gcggtgtcat
     4981 cagcgcataa ctttctgctg gagtggtatc aataatattg gcaatcatcg agcggtcgat
     5041 gacgatgccg taagtcatta tgaaatattg tgcagccgcg ccaaccagaa taaacaggca
     5101 ggccagtggt cgattaagcc ataagaaaga gcttagtgtc aggacaatat taatcacgct
     5161 gaaagcgacg accggcatcg acaagaaaac cagtacgtta tgcagcgaat ccagcggcag
     5221 cgcctgcaac acctgtttaa aaaaggcaat attcaggcag atagagatat aaaaagcggc
     5281 caacaatagc caggcgagta aattcaaaga gggtcttttt agtaggcgct tcaacatcac
     5341 ggtgtttcca tcgaacaaag tgcgcatatg ctcgcaaagc aaaattaagc caaccttaag
     5401 ttcttaaggt tggcttttat gtttgctgga ttaaggggaa ttaatctttg cttattggtg
     5461 catctaaggg atacgggttt ttatgtagcc ggttgtaatt cagcgcatac atggcggtga
     5521 tgaccatcag ggtgacaaat gaccacataa cctctttcgc cccggacccc accacggccc
     5581 aaatgcagta gaggaaggca atggtagtaa cagccagata tgccgggcgt gctttaccaa
     5641 agtgaccgtg gccgagcagc agtaacgccg cacaggtgta aagatatggc accagtgtaa
     5701 agatgaccga gacggaagaa accagaccga actctttggt cgcgtttggt gaaatgctgc
     5761 tgagctggaa gatggtcatc aaaataccga cgataatcag ccccgccact ggcgtacccg
     5821 ctttatttac acgggcaaaa atcggtggga acagtctgtc atcggcagcg gctttcgccg
     5881 tttgacccgc cagcaacgtc cagccgccca gtgaacctaa gcaacccgca gctgcgcaga
     5941 aggaaacaat ggccccagcg gtgtcaccca acgccatccg tgcggcatca ccgaatggcg
     6001 aggcagaaac gcgcagtgcg gcattaggga tcatccccat aatcgcggtg gtggagagca
     6061 catagcaaac ggcggcaatc aataccccac caatggtggc gatagggaca ttgcgtttcg
     6121 ggtttttcac cacacctgcg gcaacggagg cactttccac accgatgaac gaccacagcg
     6181 taacgttaag ggtactttga atcgcaccga aggtgcccag gccgctgacg ttccatgccg
     6241 ccatataggt ttcaccacgg aaccagaacc agccaaatac ggcaatcccg acgatgggga
     6301 tcagcgccag cacagtggca acggcctgca cgcgggtgat cattttcgga ccgacaatgt
     6361 tcagcaggac gaagatccac agcaccacga cgcaggtgat ggttaatacc aatggatctt
     6421 tcagaatcgg gaagaagtaa cttaaatatc ctacgccaat gaccaccatg gcgatattgc
     6481 cgatccagca ggccagccag tagaggacgt tggtttgata accgagaaac gggccaaagc
     6541 agcggcgggt gtaagcgtaa gaaccaccag gacttgggtc gaggaacgac attttggcgt
     6601 ataccatcga gagccccagc gcaccgataa tcgtcaccaa ccatccataa atggcaatcc
     6661 cgccagtgga ggccaggttt gcaggtaaca gaaaaacacc tgaccccata atattccccg
     6721 acaccatcag ggtgacgggg attaagccca ctttgtgagc atcaacatcc gaagacataa
     6781 ttaaactcct gcgaaggcga gcttcgtgac aataaattac gtcatatcat acgcctgcat
     6841 tcgatagttg aattattatc gaccgggtta tcgctgatgc cgttattaat atagctcttc
     6901 gttaaggtta aaaatcaggc gacagtgcgt tctctgtgct gactaacata atgcagcggc
     6961 gtcataccgt agaagtcttt aaagacagaa ataaagtacg acgtactgtt gtagccgcag
     7021 gactgtgata cctgagagat atttttaccg tccatcatta attcatttac ggcataacgc
     7081 atgcggcagg tggtgattat ttggctataa ctggtgtttt cgcttttcaa ctttttcttt
     7141 aacagacttg ggctaagaca taaacaactg gctaccatac taagattcca gtctttgtga
     7201 atatcgcttt caataatttg ataaacgctg tcacttacac aattacgtaa catatacatc
     7261 attaatgaaa ggaatttttt actgtcgaga aaacgggata gcaccgtgaa taataatgcg
     7321 cgtgttcgtt ccttttctga ctcagtttct gcgggcatca tgctgtgttg cgcggcaacg
     7381 cgaaagacgt ctggcgtcag gcatggcagg gtgagtatgg gcgtagcgct acgttgccat
     7441 actggtattt gcgagagatc tttattcaga aaccggaggt aatctttgat gatgtcgtga
     7501 ctaatatgag caaccaaagt gttgttaaga gaggagacgt cgataatatt attttcacaa
     7561 tttaataacg ccatatggtt agctttaagg ctaataggtt ctttcccatt cacccttatc
     7621 cagacatctt tttcagtcaa taaaacaata caaggttggt cgctgcaaat cctcatatat
     7681 gtactcctga gtgcgaataa aagaccagtt ctcgctaaag caaagcgata ccgggaaatg
     7741 gttatataat tgtatgatga atataaacat gcataaaaat aacgagtttt actaatatta
     7801 caaattttta tagtaatttc tcatgtacat atttttttac aggataaata acaggcaaaa
     7861 aaaagcgggt aatgactcca acttactgat agtgttttat gttcagataa tgcccgatga
     7921 ccttgtcatg cag
//
LOCUS       DBKEKI010000164         7914 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.165, whole genome shotgun sequence.
ACCESSION   DBKEKI010000164 DBKEKI010000000
VERSION     DBKEKI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.165"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(180..371)
                     /locus_tag="ACUQPX_003695"
     CDS             complement(180..371)
                     /locus_tag="ACUQPX_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058236.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(368..790)
                     /locus_tag="ACUQPX_003696"
     CDS             complement(368..790)
                     /locus_tag="ACUQPX_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HHV9058237.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            complement(837..1262)
                     /locus_tag="ACUQPX_003697"
     CDS             complement(837..1262)
                     /locus_tag="ACUQPX_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HHV9058238.1"
                     /translation="MQYAKPVTLNVEECDRLFFLPYLFGQDFLYVEASVCALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(1674..2444)
                     /locus_tag="ACUQPX_003698"
     CDS             complement(1674..2444)
                     /locus_tag="ACUQPX_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058239.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWISIYVR
                     LDAPRLATLLIKRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRFCL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSECIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRG"
     gene            complement(2489..2923)
                     /locus_tag="ACUQPX_003699"
     CDS             complement(2489..2923)
                     /locus_tag="ACUQPX_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HHV9058240.1"
                     /translation="MYGTCETLCRELAAQYTENTPLMLVVWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEEQRIESGISSAAVMEIINNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(2937..3158)
                     /locus_tag="ACUQPX_003700"
     CDS             complement(2937..3158)
                     /locus_tag="ACUQPX_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058241.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(3159..3842)
                     /locus_tag="ACUQPX_003701"
     CDS             complement(3159..3842)
                     /locus_tag="ACUQPX_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HHV9058242.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     TPPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRATQQGAAN
                     DDWFMPEAA"
     gene            complement(3918..4154)
                     /locus_tag="ACUQPX_003702"
     CDS             complement(3918..4154)
                     /locus_tag="ACUQPX_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058243.1"
                     /translation="MRRLTRRGINAACGQCPASGRARDGRFYRSRLASVTVYASPSPF
                     SDERPSSRFRGITLPSNRRRLRYSTVGLTRYRTR"
     gene            4046..4276
                     /locus_tag="ACUQPX_003703"
     CDS             4046..4276
                     /locus_tag="ACUQPX_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HHV9058244.1"
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRLCRAASAGAVKRGARRH
                     PLSEKKRAVARPSPSYFIRHNAPPCRLSTQVR"
     gene            complement(4227..5129)
                     /locus_tag="ACUQPX_003704"
     CDS             complement(4227..5129)
                     /locus_tag="ACUQPX_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HHV9058245.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEELPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            complement(5224..5499)
                     /locus_tag="ACUQPX_003705"
     CDS             complement(5224..5499)
                     /locus_tag="ACUQPX_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058246.1"
                     /translation="MFRGKRCRRAPLFTAPADAARHKRCLWSVSRVRRARDGRFYRSR
                     LASVTVYASPSPFSDERPSSRFRGITLPSNRRRLRYSTVGLTRYRTR"
     gene            5759..6730
                     /gene="parM"
                     /locus_tag="ACUQPX_003706"
     CDS             5759..6730
                     /gene="parM"
                     /locus_tag="ACUQPX_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HHV9058247.1"
                     /translation="MIKIFCDDGSTAVKLAWFENGELKTLISHNSFKEGWNTSGFGSE
                     KTYNYLVDGEKYAFDTHNPSAIVTTNIEFQYSVENRLAVHHALLQTGMEPGDVELWVT
                     LPVSEYFTTDMQENSDNIRKKIENLKKEITLNKGSVFSIKKVHVLPESLPAALTSLEE
                     DKVSDLETSLVVDLGGTTLDCGAIAGRYKNVTQVSGEPGLGVTLVTTATMNALKQAGT
                     HTSYYIADQLVRNRNADDLFEIVVNDSSKIDDVKSVIENSITRLSDRVINHLHSYKGI
                     NRIYMTGGGAELIYPAIKKAFPLLKDKVKILPDAQLALVISIAEINR"
     gene            6742..7293
                     /locus_tag="ACUQPX_003707"
     CDS             6742..7293
                     /locus_tag="ACUQPX_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HHV9058248.1"
                     /translation="MSDERRKLWLYLYPDEPQQKSAIETIEEIPQRQRSDFFRDSIVA
                     GIALSKLDKRLPALVSMLLQQDSNIDTLVRILKILFPKLDIDSTEDKSTLCAVAWQKR
                     CRDELQQGTPWSAWFAISEDEYNHHLNHPESNSQVRALWAYDENEQKKDIQDKERTPF
                     PEANTNTSSETLSNAKKLFGNSN"
BASE COUNT         2011 a         2187 c         1976 g         1740 t
ORIGIN      
        1 acgacaacat caggaacagt tccataacat aagaaagaaa cactcctgag tcagacacat
       61 cacagacggg aagggtaacg ggcgacgcgc cagcgtggcc cgcttccctt ccggcaggcg
      121 tcagcctgcc aggggttttc gcgcgggctg acaggcggcc tccaccgcct gcccctgtat
      181 cacctgtttc tgctgccctg taagatgtag tgaccgccca ggaaaaccgc ctgctgattc
      241 gccacaaaat cgcagatttc atcaacaatc tgcgcagtgg tgagcggttt ctgccccatt
      301 tcacgacgcc gcgcattaat cacggccctg taattacgca gaatttcgcg ggtttctgtg
      361 ctgatattca tgacatcgtc tccccgtcca gcgcctgtaa cacaacgtcc attgcttcgc
      421 gttcctccct gacgaatgcg tcaccgtcgc ccccattctc actcccgacg gcatacaggc
      481 gcttcatttc actgcctgcc agcgccatca ctttttccag catcaccgcc gggactgtca
      541 catgacggct ggcgcgcttt tcttccatca gttcattgac aatatcggaa acaactctgt
      601 cacatgcgcc atatacaaaa gcatcgtcca gccgctgcat gacggtttcc agctcatcgt
      661 ctgtcagact ccagtcctgg gctatgtgac gcacatcccc acgggtcagc accacggcaa
      721 aaacgcattc actgtccggc acgtctgtat ccagtacatc gcggatgatt tctttaacag
      781 tacagtacat gttctcgtct cctttacagg ggcggcattg ccgccccgtc cgttaatcag
      841 tcaagtgccg cgtaaatcgc gttgcattcg gggtgaaatt cgatgtggct ccacaactgc
      901 gcatcgcgca tcctgtacag gtgagtcagt cctgcatccc cgctgtcgtg gtacagccac
      961 aactggcggt taatcacaag ggaagtgaga atgattcctg cggcatcagc actcacggta
     1021 cggtcaaacc agttttcacc gttaaccaga tgaaaacggt cgccatccgg catcatgtaa
     1081 ccgccaccgt ccggcaggcg gatgaagtgc cagaatccgc cttcatattc cggcatcatt
     1141 tttttcgcca gcgcgcacac agacgcttcg acatacagaa aatcctggcc aaacaggtaa
     1201 ggcagaaaaa acagacggtc gcactcttca acattcagag tgacaggttt cgcatactgc
     1261 atgggatttc ctccgttgtt cacatattcc gcgacaccgg gcggtgccgt gcctccatct
     1321 ttgccgggcc tgcagggttg caagggcgca agggaagccg cagcctgacg cacacggctg
     1381 gcgggagttg gagggtgaga ccgcgaaatc tgagcgggtg aacgaccgga aaatcacggc
     1441 agacgggaag gagggaccgg gtgaccgcag cggttcacct taccttgcaa cccgcccgca
     1501 gaagcccggc tacgatggag gcacggcaca gcccgcacac gacgctgagg ccacataact
     1561 gaaaacccac aaggccatgc cccgcatggc cgggtaaagc ggagccggcg gcgacatcct
     1621 tttgctcttt ccggggaccg gcagaaaaaa agcggggatt ttccccgcca tcatcagccc
     1681 ctgatttttg ccagggcatc cgccatgctg cggatttcgg tccgggcttc ccggaccacc
     1741 tcccgcagaa caccacggtc aaaccagcgg cggggcggtt catccggtat acgcagcaca
     1801 tcgcaggcca gcccactgga caggatatgc tcaatacact cactgaccag atacatttcc
     1861 accgacgaaa aacgtgctcc ccggttcacg atggcatcga gcaccacggg caggttttcg
     1921 tcatcttcat cgagccagca ggggtgatcg ctttccttca cgaccaggca aaagcggcgc
     1981 gtctggcggg tcagcaacgt ctgaccggtg gtggtcagcg ccgcacggat acgccgcgca
     2041 tcttctgcca cctggtaaca gatgcttcgt accgcatcgt gcaccgcatc aagaacagcc
     2101 ctgatttcgt cttcggtcag ccattctccg gcatacggga aagacacctg cgttccgtct
     2161 gccggaagta ccggcagcga ctgccactga ctgccggaca gtaccagttc cgccccggtt
     2221 ccggtcagtc tctgaatggc agaggccagg agcggcggca gtacgccgcc gtgacgcttg
     2281 ataagtaacg tcgccagccg gggcgcatcc agccgcacgt aaatgctgat ccagcccgga
     2341 tacttcgggt tccgctggtc aacatccgtc tcttccggcc agccctgcgc cagcagcagg
     2401 ttgctggcgt ggcgataaat cagtgcattc agcgtttcat tcataattca tgaactccct
     2461 gtatcgccgc ccggaggcgg cgataacatc agtcgttttc gtgtgtgttg tttttcagca
     2521 gggtgcgggc ctgattaacc acctcctgac ggcgggtgac gcattccggg acggcgaggc
     2581 catgatcccg ggcggcccat tcacgtttcc acaatgcctg ttcagcggtc tgaatcaggg
     2641 acgccagcag ttcagcaggg acggtcacct ggcggttttc gctcacgtta ttgatgatct
     2701 ccatcaccgc agcggaagaa ataccggact caatccgctg ttcttccggg atgtcctcca
     2761 ggcgtgccag gacggtcctg atttcatgac cggtcagaga aatttccata ccgtcagcca
     2821 gtgcctgaat ctcttccggg gaccagacaa ccagcatcag cggcgtgttc tccgtatact
     2881 gcgctgccag ctcacggcag agcgtttcgc atgttccgta cattctcgtc tccttctcaa
     2941 tctttgaagc aggaatccag ttccagaatt tcggtatatg cctccatcag gaggcggttt
     3001 gcgcgaaaca gcgtctgacg caccagacgt tccggcaggg tgtcggcatt aaagctgtac
     3061 cgctgctcca tctcattcat ggttgtacgc aggtcgcaca tggcattggc cacctccgcc
     3121 acttcggcgc gcagtttttc gtgtcctgca tagttcattt acgcagcctc cggcataaac
     3181 cagtcatcat tcgcggcccc ctgctgcgtg gcccgttgca cggcggcaag gcgttgctgc
     3241 ccggcacggt gatactgctc aagcagctcg ataccgatat accggcgtcc ggactggagg
     3301 gcggccacgc aggttgagcc gctgcctgca aacgggtcca gcacaattgc gttcgggggg
     3361 gtgaagctct caatcagcgg ttgcaggctg gtaacgggct tttccgtcgg gtgatgacga
     3421 ttgcctgaat atttccagcc cagcacgtcc ggcagcggtt tttgtggcag acgtggacgg
     3481 ccttttgcca ggatgtaggc acattcgtgg cgatagccca catatgcggc cttcgatgtg
     3541 tagtttttgg tgaagaccag gtgaccaaca acgctgaatc ccgcattttt ccaggcagac
     3601 ataaaacgat cgacgcgatt ccagccatag aagctcacca ttaacgcgtc ttttttgagt
     3661 acgcggtaca tctcattaca ggccggttgc agccattcat cggttttatc gccggcaatg
     3721 gtacgcccct gacggtcacg aaaaccgacg aggtacggcg ggtcggtgag gatgaaatca
     3781 atggcgttat cagggatacg ggccataaca tcgatgcagt taccgaggac aaaacgggac
     3841 atacaaaact ccttctctta cagagggcac cgggagggca cccctgtggt gcccgttccc
     3901 cgctgcctgt gggctgttca ccgcgtccgg taacgggtca ggccgaccgt ggaataccgc
     3961 agccgtcgtc tgtttgacgg caaggttatg ccgcgaaagc ggcttgaagg gcgttcatca
     4021 gaaaagggcg acgggctggc gtacactgtc accgacgcca gccgggagcg gtaaaagcgc
     4081 ccgtcccgtg cccggccgga cgcgggacac tgaccacagg cagcgtttat gccgcgccgc
     4141 gtcagccggc gcagtaaaaa gaggagcacg gcgacatcct ttgtcggaaa agaaacgggc
     4201 ggttgcccgc ccgtctccgt catacttcat ccgccataat gcgccacctt gtcgactatc
     4261 cacgcaggtc cggtgacttc cggcagctca tcgtcatctg tcggggaaga ccattccagt
     4321 tccccccaca gcacatcaac gagctctccg cgctcataca actgccagcc acagaaattg
     4381 cagccctgtt cggcgtacca gtgctccagc gtgcagccga aacggcggct taattctgca
     4441 acaacgtcgc tttccggctg acaccacggc gtgtcgaaat ctacctgaat gaagttttcc
     4501 ccctgactgc tgatattcag gtcataccca caaggccact tcacgccata ccgttctgta
     4561 taccagtgat atgcagaagg aacaccgttc agaacaccgc cgttaaagcc attcacttca
     4621 atatccaggc gggtcggcaa aaccgtcagc aggtcaaacg gcatggtttt ttccggcagt
     4681 acgttgtcac acagacggtt ccaccacacc gatacatcct cgttacccca gatgtcacac
     4741 cagtcacctc tttttgcggt gaaatgcaca gtgatagttt ccctgacatc atccggtaac
     4801 tcttcccagc gacgctgtcc agtaccgctc tgcagccaga gttcatgcag catcaggcag
     4861 ttttgctcat ccagcagtac accgttctgc aggtgtgtca gccagcgggt gaacgcgata
     4921 ttttccggtg atacagcacc acgtccggca gccgtcactc cggggcactg ctcagagcgg
     4981 atattttcag tgatctgcag aagtccggca cttccggcca gaaacagctg aataccttca
     5041 tttgtggcgc ggcgataaag cggtgtgact gcaccgctgg ccagtcgttt aatctcagcg
     5101 atctgtgctg gttcaccaga aaaatacata cgattcgagc accagtttgg catgggaatt
     5161 ctcctcacag gagcaccggg agggagcccc tgtggcgccc gttccccgct gcctgtgggc
     5221 tgttcaccgc gtccggtaac gggtcaggcc gaccgtggaa taccgcagcc gtcgtctgtt
     5281 tgacggcaag gttatgccgc gaaagcggct tgaagggcgt tcatcagaaa agggcgacgg
     5341 gctggcgtac actgtcaccg acgccagccg ggagcggtaa aagcgcccgt cccgtgcccg
     5401 ccggacgcgg gacactgacc acaggcagcg tttatgccgc gccgcgtcag ccggcgcagt
     5461 aaaaagagga gcacggcgac atctttttcc cctgaacatc cgtcagcctt tctctgcggc
     5521 aagatcaatc cttaacagaa aaaaaccaac aagcacccac ataaaaaaca aaaaacccaa
     5581 gcaacaccca aacaaacaaa aaaacaccca gaaaacaccc aaagacaaac aacaataaag
     5641 taacgaaacc cataaaacac ccaaaaaaaa gacaaaaaag attaaatagc acttgaacgc
     5701 caccaaaaaa atactattat gggtatagat acccaaacga cacccaaaag aggattttat
     5761 gattaagata ttctgtgatg atggttctac agcggttaag ctggcatggt ttgaaaacgg
     5821 ggaactaaaa accctcatta gtcacaattc tttcaaggaa ggctggaaca catcaggatt
     5881 tggcagtgag aaaacttata attacttagt cgatggagag aaatatgcgt ttgatacaca
     5941 taacccatca gcaatagtca caaccaatat tgagtttcaa tatagtgtag aaaatcgtct
     6001 cgctgtacat cacgcactac tacaaacagg aatggagcct ggggatgtag agctttgggt
     6061 tacattacct gtatctgaat atttcactac agatatgcaa gagaattcgg ataacataag
     6121 aaaaaaaata gaaaacctga aaaaagaaat tacactgaat aaaggcagcg tattttcgat
     6181 caagaaagtt catgtattac ccgagtctct acctgcagcg ttgacctctc tcgaagagga
     6241 taaagtcagt gatcttgaaa cgtcccttgt cgtcgatctt ggtggaacaa ctcttgattg
     6301 tggcgctata gctggcagat ataaaaatgt aacacaagta tctggagaac ctggtcttgg
     6361 cgtaactctg gtaacaacgg cgacaatgaa tgccctgaaa caggcaggaa cgcataccag
     6421 ctactatatt gcagatcaac tggttagaaa cagaaatgct gatgatttat tcgaaattgt
     6481 tgttaatgac agcagtaaaa tagatgatgt gaaatcagta attgaaaaca gtattacaag
     6541 actttcagac agagttataa atcatttaca ctcgtacaaa gggataaatc gtatatacat
     6601 gactggtggt ggtgcggaac taatctaccc tgcaataaaa aaagcattcc ccttattaaa
     6661 ggataaagta aaaattttac cagatgcgca attagccctt gttatttcta ttgctgaaat
     6721 aaatcgataa tgaggtacag gatgagtgat gaacgtcgta aattatggct atacttatat
     6781 cctgatgagc cacagcaaaa atccgcaata gaaaccattg aagaaatacc tcaaagacaa
     6841 cgttctgatt tcttcagaga tagtattgtt gcaggtattg cattatcaaa actcgataaa
     6901 cgattacctg cacttgtatc tatgttacta cagcaagata gtaatataga tactcttgta
     6961 cgtattttaa aaatactatt ccccaagctt gatattgact caacggaaga taaatctaca
     7021 ctctgtgccg ttgcttggca aaaaagatgt cgggatgaat tacaacaagg cacaccatgg
     7081 tctgcctggt ttgcgattag tgaggatgaa tataatcatc atcttaatca tccagaatca
     7141 aacagtcagg tacgtgcatt atgggcttat gatgaaaacg agcagaaaaa ggatatacag
     7201 gataaagaga gaacgccctt tccggaggcc aacaccaata caagttcaga aacattgagt
     7261 aatgctaaaa aattatttgg taattcaaat taattattca aagaaaatcc cggtcgaagg
     7321 gccgggatgt ttacacgtta accaccctgc aattctgcca ctcctgatgc ggaataacag
     7381 gacaggaccg ggaacagtca ggctcacttt tttctccgtc gtctgccacg cgctctgctg
     7441 cctgtatttt tttcccggga tgccggctca ctctgcaatg ccgggggagg tacctgtttc
     7501 aagagctggc gcagcacact gttgaggatc agcgtccgcc gagcccgtgt ggcagccaca
     7561 taggggtttg aagcccaatg gagcggaaac gtacgctaag aagctaacta tatgatttta
     7621 agtcaataaa gtcgtggcgt tgttaggcag gtttacgttg atttgattac cgagtttatt
     7681 aacactgaaa gtccctgaaa ccgcatggct actggcattg acgggaagag taaacaaaac
     7741 cctcgttttc tttactcacc tcttctgctc aaaaaatgaa cttagcgtac gatctcgtac
     7801 ggttgggcct caaaccccat ttccggataa ctgacgatat atggggcatt caggcggttc
     7861 cggcgtagaa ccccattcag gtgcatgctc atgattaaaa ctggacagat aaag
//
LOCUS       DBKEKI010000165         7888 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.166, whole genome shotgun sequence.
ACCESSION   DBKEKI010000165 DBKEKI010000000
VERSION     DBKEKI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.166"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..128)
                     /locus_tag="ACUQPX_003708"
                     /pseudo
     CDS             complement(1..128)
                     /locus_tag="ACUQPX_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            126..450
                     /locus_tag="ACUQPX_003709"
                     /pseudo
     CDS             126..450
                     /locus_tag="ACUQPX_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(480..3381)
                     /locus_tag="ACUQPX_003710"
                     /pseudo
     CDS             complement(480..3381)
                     /locus_tag="ACUQPX_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
     gene            3864..4706
                     /locus_tag="ACUQPX_003711"
     CDS             3864..4706
                     /locus_tag="ACUQPX_003711"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HHV9058249.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAEGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4709..5797
                     /gene="capU"
                     /locus_tag="ACUQPX_003712"
     CDS             4709..5797
                     /gene="capU"
                     /locus_tag="ACUQPX_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HHV9058250.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5802..6752
                     /gene="virK"
                     /locus_tag="ACUQPX_003713"
     CDS             5802..6752
                     /gene="virK"
                     /locus_tag="ACUQPX_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HHV9058251.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6817..7761
                     /gene="lpxM"
                     /locus_tag="ACUQPX_003714"
                     /gene_synonym="msbB"
     CDS             6817..7761
                     /gene="lpxM"
                     /locus_tag="ACUQPX_003714"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HHV9058252.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2126 a         1763 c         1869 g         2130 t
ORIGIN      
        1 acaggtactg tggaggcact tccgtaaaga agggcatcaa gctcctgctc cgacatacct
       61 gcaggcaaag gccatgaaag ccctgatttc cggaagcgag cgaacagtcc acaaactgtt
      121 gaacgtggtg tcagcggcgg acagggataa gccctcgtca gcagttccgt cagcactgcg
      181 acagcgttct ttacccggtc aaaacagcgt tgcggtgcac cacgcctgac ggatgaactg
      241 cgtgctcagg gttaccactt taacgtaaaa accgtggcgg caagcctgcg ccgtcagggg
      301 ctgagggcaa aggcctcccg gaagttcagc ccggtcagtt accgcgcaca tggcctgccc
      361 gtgtcagaaa atctgctgga gcaggatttt tacgccagtg gtccgaacca gaagtgggcg
      421 ggagacatca cgtacttacg tacagatgaa gttcggttac atcctgtcag cacggaacct
      481 cacgcttttt gagcggcacg acggatcccc cccacccagt atccgacaga cttttcactg
      541 cgttcgtaaa aacccttctt ccctcccagc atatccacta attccggtga catatcctgc
      601 agataagtct gcaaatcatg ccccagatct tccggtgact gcaactcata aaccgcgcgc
      661 cacacatcac cctgccttat tttaagcccc gggttaagaa tatactgtcc gtggttcagc
      721 ctcagaaaaa gcttttttcc ataaggattt ttgctgttaa cttcatgttg agagagctgg
      781 ctgctgatat aactccttcg tttgcggcgt tccggcagca acgcttcagg aagatgaacc
      841 acacactctg ccagtcgtgc cgcactgaag gcttccgaaa aaaacagagc acttcccaga
      901 tgcgtgtgga acagcgcctg catcaggatc accagaaaca ttgccatctg tcggttgtcc
      961 agtttgatga ggcgtccttc cacctgcagt gacagacttt caggtttcag cagggtatac
     1021 aactgcgcac ttttctgttg cgcataacgg ggcgtactga ccgccgctga cagaacttga
     1081 tgataagccg ccagcccgtc attaccggtc agcaagggat ctgcaccacg ctccagcagt
     1141 aactgaacca gtgcaatatt acctgcccgg gctgctgaca tcagcggagt aagattaaac
     1201 ggcgaacggt gatcaacacc ataacgttca atatcccgca acacggcaac cgggttattc
     1261 attgagtatg gccggaaata cttcagctcc agtaactgca gagtcttcgt tctgggctgc
     1321 cttgtgggct taaaatcttc ccggtacagt gcattataca gcgggaacag ggaataaatc
     1381 agactgtact ccagcgcctg cagttgtatg gttttatcgg ctgtacctgc gaggatatgc
     1441 tgtttccact gtcgtgcatc ctctgcggtg ataatctgcc atggcatctc agatgtctgc
     1501 aggatccggc tacggatggt gtccgcctgc tcctgcttcc cctgtttttc aagcagatgt
     1561 gcggttttct gccactcatc acggctggaa atctcttcct gcagattcag aggctcttcc
     1621 tgatgcgtga gcgcaagcag actccacaga ggatgctcca cctgctgttc aacaagatac
     1681 acattgtgtg tagcacgggt gagcgcaaca tacagcgcgt tggtaaaaaa tttatagatt
     1741 tccgctgaac gatcctgcct gtcccgcgga cgggaataac gtgcctcgcc ctcaagatca
     1801 gccggtgtaa gaccctcgca aatatcatca aatgcctgcc gggcagcgct tactatattg
     1861 taaaggatca ccgtttcgta ttccagcccc ttaacctcct gaaccgaaaa gaccagcggc
     1921 gtactgaacc agcatcgcgc ctgtgctttc tgttccggat gcatcataat gaccgctaca
     1981 cgattcgaaa gtgaggtttt ggcattcagt tcctgctttg tctcttccct gtcgtccagc
     2041 aaccggatgg tcccttctgc ctgaccgcag cttctgacaa aatggtgact ttcccgatca
     2101 atagcactaa agcggacctg tttgagtctc agcaaccggt tagccagtgc agtgacgtgg
     2161 tgcccgttac ggtaattcgc ctgcaggata ttaacggttg tgtcgtttcc ctgttgctgc
     2221 cggaaaaaga gacttttgag cgaactccat gagaaaaagc tcggatgaac tatctgattg
     2281 gcatcaccac agagtaaaaa ctgaccggga tgacgtagcg ttttcagtac cagttgcagt
     2341 tgaacaggcg tcatatcctg aacttcatca acaaaaatga catcataacg gggagttgcc
     2401 aacgaaagat actgatgact gagcagatta ctgtcatact gatgcgactg tttcagccat
     2461 gcgatataac gttcaaacag gacatagacc gtcgggcggt cctccatgcc atagagcgac
     2521 tgccgtatcc ccagtgacag atacgcctcc cgggacagcg gcccattccc ggaggcaacc
     2581 gcaccgatca cgcccctgaa ctcctcatac agggtatggg cagcccccag tgcggcgata
     2641 gccctgttgc gcgctgaacg cactgaaagt cacctccctg ccttccggaa tgcgcagagt
     2701 ttccagaaat tccgtcagcg aaagaaagtc aatattctgt acctcactcc cttcccctga
     2761 agcgtcatag agagtacgcg ctttttccac cagaaatgag gaaaggctga gatacagtat
     2821 atcaccagca gcctgcttca ttttttccag taccagcgcc gtcttaccac ttcccgccga
     2881 gccaacaatc accagcggta gcgggtgttc gtacagtgcc tgctgatcat catcaaagga
     2941 cagcatttta tccaggcggt ggaaacgccc atgagacgga ttgatatacg tcagcgcttc
     3001 agtcgcgata tcaacaggat ccggcactgg ttgttgttgt aagcgccctt catcaatcac
     3061 cacgtttctg cgaagaaaat gtgaagtgtt ataggcatga ttgcgaatat attccagaac
     3121 aaggcagata gtttcaccgt ggtagcgata cagcgaaaac agtaatcggt cccggatact
     3181 gagccgggcg cggtaaagat tggtgtcaat tttacgaaca tcagcctgaa caaagttatc
     3241 ggtggcaata gcctgtgcaa attttttata acccgggatc gtgtcaggat tcagagtatg
     3301 ataaagacag attttcatgg ctgccggttt ccgtcgtgca aaaaacatgc tgattttacg
     3361 aaggaatgcc ttactgaaca accttgcccg gaataccccc cgaaatcaga tggaacaatc
     3421 agaaaaatgc tctgcagtta tggaataaag aaggtggggt ggtacgccgg gagggcgatc
     3481 cgctggaaat ggcgcagtag ctgcaggcct gtcatgatgc cggaatagaa gttcgtgtac
     3541 aggttaatga aagcgtcacc ccgtaaataa actctgcgct gaaattcctt tttttcattg
     3601 gagcacctgt gttgttctga ggtgaacata tcacctctgt tcaggtagcc aaattcagtg
     3661 tgccacctca aggttcttag tgagtcgttt ttaaaagata tgataatata tcattgttta
     3721 tgaattgttg atgtttaatt tagtaaatta tatcaaatat gccatgggtt tgctatgtta
     3781 ttcatccagt atctggaaat aaaatgcacc agctttattg ggttttcctt ttgtctctgc
     3841 ttactaggtg ccagaaactt taaatgttaa acgaaggggg cattttgttc aaagccaatc
     3901 atgttccggt attaatgtat catcatgtca gtcattgccc cggactggtt acactttctc
     3961 ccgtgacatt ccggaagcaa atgaaatggt tggctgaaaa caactggaaa acactgtcat
     4021 cagatgaact ggagtttttt tatcggggcg gaaaactccc ccggaaaagc gttatgttga
     4081 catttgatga tggatacctt gacaactggt ttcaggtata tcctttatta aaagagttta
     4141 atctcaaagc acatattttt ttaattaccg gttttattgg gaatggtccg gttcgtcact
     4201 ctcctggaaa ggaatattct catcgcgatt gcgaacatca aatagcgaca gggaatgctg
     4261 ataatgttat gcttcgctgg tctgaagtaa acgaaatgtt gcaatctggt ctggtcgaat
     4321 ttcatgttca tacgcatact catacacgct gggataaaaa gttcagttca cgagaagaac
     4381 agtgtaaaca tcttcggcag gacttgttga gcgggagaga atatttgaaa gaaatgacag
     4441 gaaaatgcag taaacatttg tgttggccgg aaggttatta taacaaggat tatattcagg
     4501 tcgctgaaga acttggattc tattatttat atacaacaga aagaagaatg aatgctcctg
     4561 ctgaaggaac tacacggata ggaagaataa gcaccaaaga acgggaaagt tgcgcatggc
     4621 tgaaaagacg acttttttat tatacaacac cgtttttttc atcgttgctg gcctttcata
     4681 aggggccaag attaccggat gattaataat gaatatacta tttacggaat catcaccgaa
     4741 tataggtggt caggaattac aggctgttgc tcaaatgaag gccctgaaga aaatggggca
     4801 ttcagttctg cttgtctgca gggaaaacag caaaattgct tttgaagcca gtaaattggg
     4861 aattgatatc acattcgcgt tatttcgaaa cagtcttcac atccctactg catggagatt
     4921 actcggaata gttcatggtt ttcagcccaa tgcaatcgtt tgtcacagtg ggcatgatag
     4981 caatattgtt ggtttagtac ggttattcac ttggaaacat ccattcagaa ttatcaggca
     5041 aaagacatat ttgacacgaa aaacaaaagt tttttcaata aatcattttt gcgatgaggt
     5101 gattgttccc ggaacaagta tgaagacaca tctggagcag gaaggatgtc gaacccgggt
     5161 tactgttgtg cctccaggct ttgatttcca gaaattatac gttgattccc gaaacagttt
     5221 gcctccaaat gttctttctt ggctggcgtc ccgaagggga tgccccgtta ttgctcaggt
     5281 aggaatgttg cgcccggaaa aagggcacga atttatgttg aatttactgt tccatttaaa
     5341 aatgaatgga cgacagttct gttggttgat tgtggggtct ggttcgcctg aactgcggga
     5401 gcatttacag tatcagattg acagtatggg catgcatgat gatgttttta ttgctgacaa
     5461 tgtttttcct gccgcccccg tatatcgggt tgccagtctg gtggttctgc cttcagaaaa
     5521 cgaatcgttt ggtatggtac tggcagaagc atcggcattt tctgtgcctg tactggccag
     5581 tcagattggt ggaatccctg atgttattca gaacaaccag accgggacat tgttaccagc
     5641 aggtaataag cacgcatgga tgtgcgccct gaatgatttt tttaatgacc ctgggcgttt
     5701 ttatcagatg gctcgccagg caaaacagga tatagaagag cggtttgata ttaataaaac
     5761 tgcgttaaaa atactcacat tagcgaagca caagtaacaa tatgttttct gtaagtaact
     5821 tatcatttat cggtttcctt aaaaggattg ttttttcctc agattcactc ccggggaagt
     5881 gggaacacag aaaattccgg ttcatgtaca ttttgcgatg tgctataaat ccggttgcca
     5941 gtattcgata ttattacgaa ctgcgttcct tgcagtgcat tgaggatatt ctggcaatac
     6001 aacccacgtt gccagcaaga attcatcgac cttatttgca taaagggggg cgagcctggt
     6061 cccgggggca atatattctt gagcattatc gctttgtgca aaatttgcca gaaaaatatt
     6121 ctgaattttt gtttccacaa aaatcggtat cattagtcca attcatcggg aaagatggag
     6181 aggattttga tatccagtgt tctcccagtg gttttgaccg ggaaggagag ttgatgttgt
     6241 cattgttttt caataaaata gtgattgccc ggttaacatt ttcagtcatt ctgactcaaa
     6301 atggacacac tgcttttatt ggcggattac agggagcccc taaaaatacc ggacctgatg
     6361 ttattcggtg cgcaacccgg gcctgttatg ggattttccc aaaacggatt atttttgaag
     6421 cattttgtgc gttgatgaaa gcttgcaata tctctgagtg tctggcagtc agtgagcaca
     6481 gtcatgtttt ccggcaattg agatactggt atcagaaacg caaaaccttt gttgcggtct
     6541 atagtgattt ctgggagtct gtagcaggaa aaacctgtgg tgactggtac agattaccaa
     6601 cccaggtgat tcgaaagccg ctaagcgata tagccagtaa aaaacgctct gagtatcgaa
     6661 aacgatacgc tttgttggat tatatccatg aaactgctat ccgctctttg gatgcatatc
     6721 ctgtaaactc agaacatcag gacttaaatt aaggtttgat gtattctctg aataaaatat
     6781 taatgatgat tttggtaggg gcattcgcac taaataatga aaaaatacaa atccgagttt
     6841 attcctgaat ttaagaaaaa ttatctttcc cctgtttact ggtttacatg gttcgttttg
     6901 ggaatgattg caggtatttc aatgtttccc ccttcattca gagatcctgt cttggccaaa
     6961 atagggcgtt gggtgggcag attgagcaga aaagctcgtc gcagggcgac gattaattta
     7021 tcgctttgtt tcccggaaaa gagtgataca gaacgggaaa taattgtcga caatatgttt
     7081 gccacagcat tgcaatccat agtgatgatg gcagaactgg caattcgtgg tccggaaaag
     7141 ttccagaaac gagtgttctg gaaaggcctc gaaattcttg aggaaatccg gcacaataac
     7201 agaaatgtga tttttctggt tccccatggc tggagcgtgg atattcctgc aatgttgctg
     7261 gcagctcagg gggaaaaaat ggccgccatg tttcatcagc aacgaaatcc agtgattgat
     7321 tatgtctgga attctgtacg gcgtaaattc ggggggcgct tacattcccg ggaggatggg
     7381 ataaaaccat ttattcagtc agtacgccag ggatactggg ggtattacct tccagatcag
     7441 gatcatggtc ctgaatacag tgaatttgct gatttttttg cgacctataa agcgacatta
     7501 ccaattattg gacgtctgat gaacatcagt caggctatga ttataccgct tttcccggtt
     7561 tatgatgaaa aaaaacattt cctgactatt gaagttcggc caccaatgga tgcatgcatt
     7621 gccagcgcgg acaataaaat gattgcccga caaatgaaca aaacagtgga aattttggtg
     7681 gggtcacatc cggaacagta tatctgggtt ttaaaattgt taaaaacgcg caaatcaaac
     7741 gaagcggacc cgtacccttg aaattataaa gtacaggtat ttccactagt tgtttcttac
     7801 aggttaccaa tcgaaacaca tcccccttcc gtgtgttgcc cgtttggcac ggaggactgt
     7861 gtagatgcag ttcgttaaat ttaattta
//
LOCUS       DBKEKI010000166         7843 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.167, whole genome shotgun sequence.
ACCESSION   DBKEKI010000166 DBKEKI010000000
VERSION     DBKEKI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7843
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.167"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..638)
                     /gene="yihS"
                     /locus_tag="ACUQPX_003715"
     CDS             complement(<1..638)
                     /gene="yihS"
                     /locus_tag="ACUQPX_003715"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HHV9058253.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNN"
     gene            complement(655..1533)
                     /gene="yihT"
                     /locus_tag="ACUQPX_003716"
     CDS             complement(655..1533)
                     /gene="yihT"
                     /locus_tag="ACUQPX_003716"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HHV9058254.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(1557..2453)
                     /gene="yihU"
                     /locus_tag="ACUQPX_003717"
     CDS             complement(1557..2453)
                     /gene="yihU"
                     /locus_tag="ACUQPX_003717"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HHV9058255.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVARTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            2621..3517
                     /gene="yihV"
                     /locus_tag="ACUQPX_003718"
     CDS             2621..3517
                     /gene="yihV"
                     /locus_tag="ACUQPX_003718"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HHV9058256.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITSQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            3551..4336
                     /locus_tag="ACUQPX_003719"
     CDS             3551..4336
                     /locus_tag="ACUQPX_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HHV9058257.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            4435..5034
                     /gene="yihX"
                     /locus_tag="ACUQPX_003720"
     CDS             4435..5034
                     /gene="yihX"
                     /locus_tag="ACUQPX_003720"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HHV9058258.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            5028..5900
                     /locus_tag="ACUQPX_003721"
     CDS             5028..5900
                     /locus_tag="ACUQPX_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HHV9058259.1"
                     /translation="MLKTIQDKARHRTRPLWVWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            5897..6334
                     /gene="dtd"
                     /locus_tag="ACUQPX_003722"
     CDS             5897..6334
                     /gene="dtd"
                     /locus_tag="ACUQPX_003722"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HHV9058260.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            6379..7320
                     /gene="fabY"
                     /locus_tag="ACUQPX_003723"
                     /gene_synonym="yiiD"
     CDS             6379..7320
                     /gene="fabY"
                     /locus_tag="ACUQPX_003723"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HHV9058261.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            7736..>7843
                     /locus_tag="ACUQPX_003724"
     CDS             7736..>7843
                     /locus_tag="ACUQPX_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058262.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQI"
BASE COUNT         1838 a         1949 c         2093 g         1963 t
ORIGIN      
        1 tgattatttc tggcgacgtc gtggataatc acggaagcca cgcgaatcgc gcgatccagc
       61 cattttttgt cgtgagtgac gtcataaaca atcaagaaag cttccaccgc gtgcatattg
      121 gcattgccgc cgcggtactc ttcggttttg ctgaaggctt cgtcccagga ttccaggcac
      181 atctgctctt cttcgctcca gaaatatttc tcgataatct caatggtgta atcgagcagc
      241 ttgcgcgctt ccgggtgacc cgttgtgacg gcgctggcgg cacccagcag agcaaagaag
      301 tgttgatagc cctgtttgga ggcatccacc acgccctcgt cattcacgca ggcataccag
      361 ccgccatatt ttttatcacg cagtgcgccg ttcatggctt taataccgtg atcaaccaac
      421 gcgtaagcgc caggtcgacc catcgccgca gcaacggaat aaacgtgcaa catacgagct
      481 gtgatccaca gatgggtgcc catctcttct ttgatttgcc ctttattgcc taaccagcca
      541 aaaccagtcg gcactacgga atttttacca aaatcaaaga tgcggtcggt ttcctgttcc
      601 agccaacggt tgtggcttag ggtgttaaac catttcattc cttttatcct catcttagcg
      661 gcgtttagcc atcatttcgt cgacgatatc gccaagttgt tgtaatttcg gtgcgcaaac
      721 gtcacggagc atcagctcgt tgtctggtaa accgacgacc gatgcccaga ctgcacggcc
      781 tgccaggaat cccgatgcgc ccgccgtcat tgccacgcgt acggcacgcg ggaacagttt
      841 ttcgtcgaca ccggaagaga ggatcaccca tggcatattg atatggtcat tcagacgttg
      901 tgaagcacag agaagctctt gttgcggacc tttgccataa agcggcattt caactttgta
      961 gaggtcagcg ccactgtcgc ccagctcttt ggcggcatcg atgatcgctt gttcgcgatc
     1021 gaatttatcg ccacgacgcg gtgggcggac gactggctca atgatgctta ccagaccgtg
     1081 tgagtggcac agttcgttga actctttcac catatccaga cgttgctgcg catcttcatc
     1141 actacgccac agcaccagca gttttaaggc tttgccaccg tcttgtttga tctgtagcgg
     1201 attgattttg cggtcaataa ccacgctatc gaccggaata ccgttgccag gaatgaactc
     1261 atcggcggcg acaatcatgg cgcaactttt ggcaatcgcg ttttgctcaa ccacctggcg
     1321 gtagcagaat tgttgatcta ccagaatcgc cgaggcataa ggcgagaggg cctttgcagc
     1381 gttaactttg aaatcagtta aaacgctatc ggctaccgga gcgggtgccc cagccgcggc
     1441 aaacatcatg cgcatggctt cgcgctgatc gaccgccagc atggcaaaac cgcccgatgc
     1501 gcgcgtaatg tcgttgatgg tgtacttatt cattcattta ttcctttatc cagtcgttac
     1561 atttttactt tggcagtcat cccggcactg acacggacct gttccagaat ggcggaccag
     1621 tcctggcgac cgcgacccgc tgcgcgcgcc tggctataaa cctcccgtga ggcggccccc
     1681 agcggcattg gcacatgcag ctggttggcg acatcaaggg cgatgccaag atccttatgg
     1741 gcaagatcga tcatgaaggc gggagaaaga tctccgctga ggactttgtt tggccaggaa
     1801 gtggtgaagt gacctttacc ggcggcggta ccgctcatca ctttgacggc aacatcgaag
     1861 ggaagattca gggcttcgca caaaacggcg gcttctgccg aaagcgcatt gagcgcgatg
     1921 ctcatgtagt tgttgatgag cttaacgcgg atccccatgc ccggaccgcc agcgttgatc
     1981 aactcactgc ccatcgccat caggatcggt gtggcacgtt caacttgttc agcggtgccg
     2041 ccagccagca gtaacagagt accggtaatg gcatttgcag aagtacgggc taccggaaca
     2101 tccatcatgc tgaagccttt ggcttgcata tcggcaatca atttatcggt ttgcagcgga
     2161 tggatggtgg acatatcaat gaccagcgca tcggtagata agccttcgca aacgccgttt
     2221 tcaccgaaca acacgttgcg caccagatcg ccattcggca acatggtaat gataaattcg
     2281 gcatctttag cggcctgcgc cgggttggcg gcgggagtcg cacctttgtc taccagatgc
     2341 cgcacagctt cggcgttcac atcaaagacg cgaagttgat gcccttgctg caataaattg
     2401 ctcgccattg gcgaacccat ttgtcctaaa ccgataaatg cgattgctgc cataaccctc
     2461 tcctgaatac agtgatgtca ctttttgtca tttatgacat gctttgcttg tctgtttttg
     2521 atcgcatttg taatttatcg tcaaaaaatt gacagccgtc actttttaaa caattggtga
     2581 aattaaaatg aacgcatccc aaaatgttta aggaatgacc atgattcgtg ttgcttgtgt
     2641 aggtataacc gtgatggatc gcatctatta cgtggaaggg ttaccgacgg agagcggtaa
     2701 atacgtggcg agaaattata cggaagttgg tggcgggcca gcggcgacgg cagcggttgc
     2761 ggcggcaagg ctgggggcgc aggtcgattt tattggtcgc gtaggtgatg acgacgcggg
     2821 caacagcctg ctggcagagc tggaatcctg gggcgttaac acccgttaca ccaaacggta
     2881 taaccaggcg aaatcttcgc aatccgccat catggtggat accaaaggcg agcggataat
     2941 cattaactac cccagcccgg atctgctgcc tgacgcagag tggttggagg aaattgattt
     3001 ctctcagtgg gatgttgtgc tggcagatgt acgctggcac gacggcgcta aaaaagcctt
     3061 caccctggcc cgtcaggcgg gtgtgatgac cgttctggac ggggacatta cgtcgcagga
     3121 tatcagtgag ctggtggcat taagcgatca cgcggccttt tcagaaccgg gtctggcgcg
     3181 cttaacgggc gtgaaagaga tggccagtgc gctaaaacag gcacaaacgc tcacaaatgg
     3241 tcatgtctat gtgacccagg gtagcgcagg ctgcgactgg ctggaaaatg gtgggcgtca
     3301 gcatcaaccg gccttcaaag ttgatgtggt agataccaca ggtgcgggtg atgtttttca
     3361 cggcgctttg gcggtggcgc tggcaacaag tggggattta gcggagtcag tccgcttcgc
     3421 cagcggtgta gcggcgttaa aatgcacacg tcccggtgga cgagccggga tccctgactg
     3481 tgatcaaacc cgatcttttt tgtcactttt tgtataaaat gccagggtga tggtttttcg
     3541 aggaattttc atgagcctta ccgaactaac cggtaacccg cggcacgacc aactcctcat
     3601 gctgatcgcc gagcgtgggt atatgaatat tgatgagctg gcaaatctgc tggatgtctc
     3661 cacgcagacg gtccgccggg atattcgtaa attaagcgag caaggcctga ttacgcgcca
     3721 tcacggtggc gcgggtcggg cttccagcgt cgttaatacg gcgttcgagc agcgtgaggt
     3781 ttcgcaaacc gagaaaaaaa aagcgattgc cgaagcggtg gcagactata ttcctgatgg
     3841 ttcaacaata tttatcacca ttggtacgac tgttgagcat gttgcccggg cgttacttaa
     3901 ccataatcat ttgcggataa tcaccaacag cctgcgtgtg gcgcatattc tttaccacaa
     3961 cccgcgcttt gaagtgatgg tgcccggcgg tacgttgcgc tctcataata gcgggatcat
     4021 tggcccttca gcggcgtcct ttgtggctga ttttcgtgct gattatctgg taacaagcgt
     4081 tggggcgatt gagagcgatg gcgcgttgat ggagtttgat gtaaacgaag ctaacgtggt
     4141 gaaaacgatg atggcgcacg cgagaaatat tctgctggtc gccgatcaca ctaagtatca
     4201 tgcttcggcg gcggttgaaa ttggtaacgt ggcacaggtc actgcgctct ttaccgacga
     4261 gctgccgccc gctgcgctaa aatcacgctt acaagacagc caaattgaaa tcattcttcc
     4321 ccaggaagac gcgtagattt ttgtgaccgt taacctggct tcataccttg ccacatagcc
     4381 aaacccatcc tttcccgcta cagttaattt cttgtggcgc gaaaggaggc aaaaatgctc
     4441 tatatctttg atttaggtaa tgtgattgtc gatatcgact ttaaccgtgt gctgggagcc
     4501 tggagcgatt taacgcgtat tccgctggca tcgcttaaga agagttttca tatgggggag
     4561 gcgtttcatc agcatgagcg tggggaaatt agcgacgaag cgttcgcaga ggcgctgtgt
     4621 catgagatgg ctctaccgct aagctacgag cagttctctc acggctggca ggcggtgttt
     4681 gttgcgctgc gcccggaagt gatcgccatc atgcataaac tgcgtgagca ggggcatcgc
     4741 gtggtggtgc tttccaatac caaccgcctg cataccacct tctggccgga agaatacccg
     4801 gaaattcgtg atgctgctga ccatatctat ctgtcgcaag atctggggat gcgcaaacct
     4861 gaagcacgaa tttaccagca tgttttgcag gcggaaggtt tttcacccag cgatacggtc
     4921 tttttcgacg ataacgccga taatatagaa ggagctaatc agctaggcat taccagtatt
     4981 ctggtgaaag ataaaaccac catcccggac tatttcgcga aggtgttatg ctaaaaacca
     5041 ttcaggacaa agccaggcac cgtacccgtc cactatgggt ctggctaaaa ctactctggc
     5101 aacgtattga tgaggacaac atgacaaccc tggcaggtaa ccttgcctat gtgtcgttgc
     5161 tctcattagt gccgctggtt gccgttgttt ttgcgctttt cgccgctttt cccatgtttt
     5221 ccgacgtcag cattcagtta cgtcacttta tttttgccaa ctttctgcct gctactggcg
     5281 atgttattca gcggtatatc gaacaatttg ttgctaattc caacaagatg accgccgttg
     5341 gggcgtgcgg gctgatcgtc accgcgttat tgttgatgta ctccatcgat agcgcgttga
     5401 ataccatctg gcgcagtaaa cgagcgcgac caaaaattta ctcgttcgcc gtgtactgga
     5461 tgattttaac gctggggccg ctgctggcag gggccagtct ggcgatcagt tcctatttgc
     5521 tctccctgcg ctgggcgagc gatctcaata ctgtcatcga caacgtgctg cgtatttttc
     5581 cgttgctgtt gtcgtggatc tccttctggt tgctgtacag cattgttcct accatccgcg
     5641 tacctaaccg cgacgcgatt gtcggcgcgt ttgtcgccgc actcctgttc gaagcaggaa
     5701 agaaaggttt cgcgctttat atcaccatgt tcccgtcata tcagctcatt tacggtgtgc
     5761 tggcggtgat ccccattctc tttgtttggg tctactggac atggtgtatc gtcttgcttg
     5821 gcgcggaaat tactgtcact ctcggggaat accgcaaact aaaacaagca gctgaacaag
     5881 aagaagacga cgaaccatga ttgcattaat tcaacgcgta acccgtgcca gcgtcaccgt
     5941 ggagggagaa gtgacgggcg aaattggcgc gggacttttg gtgttattgg gtgtcgaaaa
     6001 ggatgacgac gaacagaaag caaaccgtct gtgcgagcgt gtgctcggct accgcatctt
     6061 tagcgatgcc gaaggcaaga tgaatctcaa cgtgcaacag gcgggcggca gtgtactggt
     6121 ggtttcccag tttaccctcg ccgcagatac cgaacggggg atgcgcccaa gtttctccaa
     6181 aggcgcatca ccggatcgcg cagaggcgtt atatgactat ttcgtcgaac gctgccgcca
     6241 gcaagagatg aacacgcaaa caggacgctt cgctgccgat atgcaggtat cgctggtcaa
     6301 tgatggcccc gtgacattct ggttgcaggt atgagccagc ttccagggtt gtcacgggaa
     6361 acaagagaga gtatcgctat gtatcacctt caggttccac aaacagaaga agagttagag
     6421 cgttactatc agtttcgctg ggaaatgttg cgtaagcccc tgcatcaacc aaaaggttcg
     6481 gaacgcgacg cgtgggatgc gatggcgcat caccagatgg tcgtcgacga gcagggtaat
     6541 ctggtggcgg taggccgatt gtatattaat gccgacaatg aagcgtccat tcgctttatg
     6601 gccgttcacc cggacgtgca ggacaaaggg ttaggaacgc tgatggcgat gaccctggag
     6661 tcggtggcgc gtcaggaagg cgttaagcgc gtgacctgta gcgcccgtga agacgcggtg
     6721 gagtttttcg ccaagctggg gtttgttaat cagggagaaa tcaccacgcc aaccaccacg
     6781 ccgattcgcc attttttgat gattaagccc gtcgccactc tggatgatat tttgcatcgc
     6841 ggcgactggt gcgcgcagct gcaacaggcg tggtacgaac atatcccgct tagtgaaaaa
     6901 atgggcgtgc gtattcaaca atataccggg caaaaattta tcaccaccat gccggaaacc
     6961 ggcaatcaga atccgcacca tacgctgttt gccgggagtt tattctcgct ggcgacgctc
     7021 accggttggg ggcttatctg gctgatgctg cgtgaacgcc acctcggcgg aacgattatt
     7081 ctggcggatg cgcatatccg ctacagcaaa ccgattagcg gtaaacccca tgcggtagcc
     7141 gaccttggtg ccttaagcgg cgatctcgac cgtctggcgc gcggacgaaa agcacgggtg
     7201 cagatgcagg tcgaaatctt tggcgacgag acgccgggtg cagtgtttga aggcacgtat
     7261 atcgttctgc ccgcgaagcc atttggcccg tatgaagagg gcgggaacga agaagagtag
     7321 cttatggaga ggggcggtgc tgcctctctc attcattccg gtcatttaca tcagtccctg
     7381 actattccgg ttatattgaa taagaacttt ataaattttt attcttcgtt ggcaccatac
     7441 gtgtcttgct acatcactgt tcaggagcat ttaaatatta tttgtagagc tgagtggaaa
     7501 ttattgcaga taatgcttaa ctcatcatct tagttcaggt ttgagcgatt tttgatagtc
     7561 aaaaccccct ctggtgccat acttttttca taaatatttt taaaatctat agctcacaaa
     7621 cattatgcaa agataatggg ctggcataca ttgtcagtat cattcattta ctgctctgta
     7681 tgttatgaat cattatgttg tatgtttgct cttaaaccgt gggaaggagg ctataatggt
     7741 tgccaatatt aatctcatta aacaagaaag ttattctgtt gttaatttag aaaaacaact
     7801 aatgagtaat aaatcaccga agcaaacgct attaaatcaa att
//
LOCUS       DBKEKI010000167         7770 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.169, whole genome shotgun sequence.
ACCESSION   DBKEKI010000167 DBKEKI010000000
VERSION     DBKEKI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.169"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..1081
                     /gene="espL4"
                     /locus_tag="ACUQPX_003725"
                     /pseudo
     CDS             1..1081
                     /gene="espL4"
                     /locus_tag="ACUQPX_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(1050..2786)
                     /gene="aceK"
                     /locus_tag="ACUQPX_003726"
     CDS             complement(1050..2786)
                     /gene="aceK"
                     /locus_tag="ACUQPX_003726"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HHV9058263.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2970..4274)
                     /gene="aceA"
                     /locus_tag="ACUQPX_003727"
     CDS             complement(2970..4274)
                     /gene="aceA"
                     /locus_tag="ACUQPX_003727"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HHV9058264.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4304..5905)
                     /gene="aceB"
                     /locus_tag="ACUQPX_003728"
     CDS             complement(4304..5905)
                     /gene="aceB"
                     /locus_tag="ACUQPX_003728"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HHV9058265.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6174..7103)
                     /gene="metA"
                     /locus_tag="ACUQPX_003729"
     CDS             complement(6174..7103)
                     /gene="metA"
                     /locus_tag="ACUQPX_003729"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase MetA"
                     /protein_id="HHV9058266.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7260..7703
                     /locus_tag="ACUQPX_003730"
     CDS             7260..7703
                     /locus_tag="ACUQPX_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HHV9058267.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         1921 a         1942 c         1961 g         1946 t
ORIGIN      
        1 tacatagaac agaaatgttg actgcctata atagtattaa cctccccggt ttatatttgg
       61 ctataaatta tggcaatgcg gatatcgttg agactatttt caattcattg tcagaaacag
      121 gatatgaagg gttactctcg aaaaaaaatc tcatgcatat tctggaggca aaagataaaa
      181 atggtttttc tggattattt ttagcgatat cacgtaagga taaaaatgtt gtaacctcga
      241 ttctgaacgt cttacctaaa ctggccgcaa cacatcattt agataatgaa caggtgtata
      301 aattcctgag tgccaaaaat agaacgtcca gccatgtttt ataccatgtt atggcgaatg
      361 gtgatgccga catgctgaaa attgttttgg tcgcgttacc tttgttaatt cgcacatgtc
      421 atttgactaa agaacaggta ctcgatctcc tgaaggcaaa ggatttttat ggttgcccaa
      481 gactatacct ggcgatgcaa aatggacata gcgatatcgt gaaagttatt cacgaagcat
      541 tgccctgtct ggcccaggaa attaacattt cagcttccga tattgtagat cttctgaccg
      601 ctaaaagtct tgcgcgcgac acgggtttgt ttatggccat gcagcgcgga catatgaacg
      661 ttattaatac tatttttaac gcattaccca ctctttttaa tacgtttaaa ttcgataaaa
      721 aaatatgaag cccctcctcc tggcaaataa ttctaatgaa tatcccggtt tgttttcagc
      781 gatacagcat aaacaacaaa acgttgtaga gacggtttat cttgctttat ctgaccatgc
      841 acgcctgttt ggatttaccg ctgaagatat tatggatttt tggcaacaca aagcgccaca
      901 aaaatactct gcctttgagt tggcttttga atttggtcac cgggttattg ctgaattaat
      961 ccttaataca ttaaataaga tggctgaaag ctttggcttt acggataacc ctcgatacat
     1021 tgcggagaaa aattatatgg aagctttact caaaaaagca tctccccata ccgtacgcta
     1081 aatctttgcc tgcgccgata cgcataaaca tcttccacat gcccttcacg tatgcggttt
     1141 tgtagtgcgc gccagtaatc agcgcggaac aggtcggcgt gcatctcttc aaacagcgga
     1201 ccaatacgcg ggtcggcgca tagccagtgg cgaaactctt ccgggaaaac atcgcccggc
     1261 gagacgctgt accacggttc gctggcaagt tcgtcttccg gatagcgcgg cggcgggatg
     1321 tcgcggaaat tcacttccgt catgtagcaa atttcatcgt aatcataaaa aaccacacgc
     1381 ccgtgacggg tgacaccgaa gtttttaaac agcatgtcgc cagggaaaat gttagcagcg
     1441 gcaagctggc gaatagcgtt accgtattct tcaatggcgt cgcgcaactg ctgaccttcc
     1501 acttgttcca gccagatatt gagcggcacc atccgccgct cgatatagag atggcgaatt
     1561 acaatctgct caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt
     1621 aataccgggg aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc
     1681 attcggccca cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca
     1741 gacatctctt tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc
     1801 ggcagcgtaa acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca
     1861 ttacagccct gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg
     1921 cagccgatag ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac
     1981 tcgaccagcg ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa
     2041 acaatgctcg cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc
     2101 gtctggtgga tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc
     2161 agccaggcgg ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg
     2221 aggttgtctg tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt
     2281 ttattctgcc agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc
     2341 cagccgtgat cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc
     2401 tctggctgag agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac
     2461 cgacagtaca cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac
     2521 agccgcgtgt aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta
     2581 gtaatgcagc gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg
     2641 tttttcatcg cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg
     2701 gaagtcactt cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca
     2761 atcagtaatt ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga
     2821 tgcggcgtaa acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc
     2881 gcaagcgtcg catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg
     2941 gcctacagtc agcaacgttt gtaggttgct tagaactgcg attcttcagt ggagccagtc
     3001 agcgcggtga ctgaagacgt gccgccctga ataatagtcg tcactttatc gaagtaacct
     3061 gtacccactt cctgctggtg agatacgaag gtatagccat ctttcgcggc ggcaaattcc
     3121 ggctgctgca ctttctcaac atagtgcttc ataccttcgc cctgcgcata agcgtttgcc
     3181 aggtcgaaca tgttaaacca catgctgtgg atacctgcca gggtgatgaa ctggaacttg
     3241 tagcccatat ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc gaggtttttc
     3301 tgccagttga acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg
     3361 atagcttgtg caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccagact
     3421 aggtcagcat atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga
     3481 gtacggaaga agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg
     3541 caatctgagg tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga
     3601 acgcccgtca cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt
     3661 ggcactaaaa ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg
     3721 aagtgaactg ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg
     3781 acaccgccaa aaccggcttc cgcatcggca acgatcggca ggaagtaatc gacatagcgc
     3841 ggatcgcccg gctcaatgcc cacggaccat tggatctgat cggcacgacg gaaggtgttg
     3901 ttgatccgct ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac
     3961 atgctggccg ccaggttagc gtccgccgct acctgccatc ccgacagata gactgcttca
     4021 atacccgctt tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gctgttgatg
     4081 tagccttttt tcgactcacc gtgcagcaga cgccacattt tcgctgcgcc cagttgcgcc
     4141 agcgtgcatt caggattgac tgaaccgcgt aatttcacca catcttccgc actgtatggg
     4201 cgagtaatgc cttcccaacg cggttgagtc cactctttct gtaattcttc aatttgttgt
     4261 gtacgggttt tcatgtgcag atgctccata gttatgtggt ggtttacgct aacaggcggt
     4321 agcctggcag ggtcaggaaa tcaattaact catcggaagt ggtgatctgt tccatcaagc
     4381 gtgcggcatc gtcaaaacgg ccctgggaga aacgttcttc gcccagttcg ctggcaatga
     4441 ctttcatctc ttcgcccagc atctggcgga acaaggcttt ggtcaccggt ttgccattgc
     4501 tcaacgtttt ttgatgatgg atccactgcc agatcgaggt acgggaaatt tcagccgtcg
     4561 ccgcatcttc catcaggcca taaatcggca cgcagccgtt gccggagatc cacgcttcga
     4621 tgtactgcac agccacgcga atgttggcgc gcataccttc ttcggtgcgt tcaccatcac
     4681 aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg ttcgcgcaat acttcaagct
     4741 gatttttacg ggagccgaga atgtcgttga ataccgccat tgccgtgtcc gcaaggcctg
     4801 ggtgagcgat ccatgtgcca tcgtgaccgt tattggcttc cagcgcttta tccgctttta
     4861 ctttgttgag cacctggtta ttacgctctt catctttgct cggaataaac gctgccatgc
     4921 cgcccatcgc aaaagcaccg cgtttatggc aggttttaat cagcaggcgt gagtaagcat
     4981 tcaggaatgg tttatccatc gtcacggcct gtctgtctgg caggacacga tcgggatagt
     5041 ttttcaacgt tttgatatag ctgaagatgt aatcccagcg accgcagttc agaccaacaa
     5101 tatggtcgcg cagcgcgtga aggatttcgt ccatctggaa cacggcgggc agcgtttcaa
     5161 tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa gcgatcttct gcatagctga
     5221 agacttcgct ccaccaggcc gcttcctgcc aggactgggt tttcggcaga tagaaatagg
     5281 gaccgctgcc ctttgccaac agtgcctgat agttgtggaa gaaatagagc gcaaaatcaa
     5341 acaggctacc ggggattgcc tcaccacgcc aggtgacatg tttttccggc aagtgcagac
     5401 cgcgtacccg acaaatcaaa accgctggat tgggcttgag ctgataaatt ttgcctgctt
     5461 cattggtata gctgatggta ccgttaaccg catcgcgcag gttaatttgc ccgtcgatca
     5521 ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc cataaagact ttcacattgg
     5581 cgttcagtgc gttgatcacc atcttgcgct ctaccgggcc agttatctct acgcggcggt
     5641 cttctaagtc cgcaggaatc ccgcgaattt tccaatcagc atcgcgaatg gaagccgttt
     5701 ccgaaataaa atcaggcaac gttccgttat caatatcttg ctgctgctga atgcgcgctg
     5761 ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag ctcagtcaga aattctaccg
     5821 cttcggcagt aagaatttgc ttctcctgct cgccatacgg ccttatgaaa gccagttcat
     5881 cggttgttgt tgcctgttca gtcatcgtgc agctcctcgt catggatccg aaaacttccc
     5941 ctctgtgaac gaaggaacgt tgtgcgcttt tcgttcagca caactaagac tactcattta
     6001 aaatgcaaaa tcaaaaacaa tttccatttt aattaaaaat acttgataac ttattgataa
     6061 caatatgaat aaaatttatt cacttgctga ggtgcgtttc ggaaataaaa aaggcacccg
     6121 aaggtgcctg aggtaaggtg ctgaatcgct taacgatcga ctatcacaga agattaatcc
     6181 agcgttggat tcatgtgccg tagatcgtat ggcgtgatct gatagacgta atagttgagc
     6241 cagttggtaa acagcaaatt accgtgacta cgccagctcg ctcgcggttt gttttgcgga
     6301 tcgttgtgcg ggaaatagtt atacggtaca tccgggtcta gtccggcttc cacatcgcgg
     6361 aaatattcct gcgccagcgt ttgcgcatca tattcgggat ggcccgtcac aaaggcaatg
     6421 cgcttatctt tgctggcaaa cagatatgca tccccttctt ccgtctctgc cagaatttcc
     6481 agatcggtgt aatcacgaat caacgctgcc ggaaagtcag catagcgcga atgcggtgcc
     6541 aggaatgaat catcaaagcc acgcgtcaga agcgcatgag gatggagaat atgatgctcg
     6601 taaacgccag agagtttgtc ggtgcgagtt tgcttaggaa tgccgtagag gatattgagc
     6661 gcggcctgta ccgcccagca gacaaacagc gtcgaggtga cgtgatcttt cgaccactcc
     6721 agcacctgtt tgatctgcgg ccagtaagcg acatcattaa actccaccag gcccagcggc
     6781 gcaccggtta caatcagacc gtcaaagttc tgttcctgaa tatcttcaaa gttacagtag
     6841 aagttgttca gatgctctgc gggcgtgttg cgcgattcac gggaatcgat gcgcaacagc
     6901 tgaatatcga cctgcaaagg tgagtttgaa agcaggcgca gaaactgatt ttcagtttca
     6961 atcttcttcg gcatcaggtt aaggatcaga accttaagtg gacgaatttc ctgaccagac
     7021 gcacgagaag ttgtcatcac aaagacgttt tcttcacgca agaaattgac ggcgggtagc
     7081 tcgtccggca cacgaatcgg cataacctga ttacctcact acatacgctt atacgtttag
     7141 acatccagat agctaaagat aaccagaaaa tttgccaatg tcgagcctgc atgttgaagg
     7201 tgagaaagtt tcaaggaaaa ggctacgtta gaatataaga atgacgaaaa ggagagaaaa
     7261 tggttattag tattcgccgc tcacggcatg aggaagggga ggaactcgtt gcgatttggt
     7321 gtcgatctgt cgatgccacc cacgattttt tatcagcaga gtatcgggcc gagctggaag
     7381 agctggtgcg ttcctttctg ccggaagcac cgttgtgggt cgcggttaat gaacgggagc
     7441 agccggttgg gtttatgttg ttaagtgggc agcatatgga tgcgctgttt atcgatcctg
     7501 atgtgtgcgg ctgcggcgtg ggtcggatgc tggtggagca tgcgctctcg atggcaccgg
     7561 aactgacaac caacgttaat gagcaaaatg agcaggcggt tgggttctat aagaaggtgg
     7621 gttttaaggt tacgggacgc tctgaggtgg acgatttggg gaaaccgtat ccgttgctga
     7681 atctggcgta tgtgggggaa taagccgccc ctgccgtgcc tgcccaagct ggagaagacg
     7741 cgccagcgtc gcacccggca tttttttcac
//
LOCUS       DBKEKI010000168         7757 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.170, whole genome shotgun sequence.
ACCESSION   DBKEKI010000168 DBKEKI010000000
VERSION     DBKEKI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.170"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..846)
                     /locus_tag="ACUQPX_003731"
     CDS             complement(<1..846)
                     /locus_tag="ACUQPX_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HHV9058268.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSR"
     gene            954..1232
                     /locus_tag="ACUQPX_003732"
     CDS             954..1232
                     /locus_tag="ACUQPX_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058269.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="ACUQPX_003733"
     CDS             complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="ACUQPX_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HHV9058270.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="ACUQPX_003734"
     CDS             complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="ACUQPX_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HHV9058271.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3386..3718
                     /gene="hdeA"
                     /locus_tag="ACUQPX_003735"
     CDS             3386..3718
                     /gene="hdeA"
                     /locus_tag="ACUQPX_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HHV9058272.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3834..4160
                     /gene="hdeB"
                     /locus_tag="ACUQPX_003736"
     CDS             3834..4160
                     /gene="hdeB"
                     /locus_tag="ACUQPX_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HHV9058273.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4224..4870
                     /locus_tag="ACUQPX_003737"
                     /pseudo
     CDS             4224..4870
                     /locus_tag="ACUQPX_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4912..5442)
                     /locus_tag="ACUQPX_003738"
     CDS             complement(4912..5442)
                     /locus_tag="ACUQPX_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9058274.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5598..6164)
                     /gene="slp"
                     /locus_tag="ACUQPX_003739"
     CDS             complement(5598..6164)
                     /gene="slp"
                     /locus_tag="ACUQPX_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HHV9058275.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6411..7634)
                     /locus_tag="ACUQPX_003740"
     CDS             complement(6411..7634)
                     /locus_tag="ACUQPX_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058276.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSENKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         2241 a         1532 c         1624 g         2360 t
ORIGIN      
        1 gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt caccatttgg
       61 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg tcgggtcgga
      121 gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga gctgtactgg
      181 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
      241 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
      301 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
      361 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
      421 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
      481 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
      541 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
      601 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
      661 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
      721 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg agagtgtgac
      781 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
      841 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
      901 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
      961 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     1021 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     1081 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat caagtcgttt
     1141 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     1201 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     1261 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     1321 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     1381 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     1441 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     1501 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     1561 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     1621 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     1681 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     1741 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     1801 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     1861 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     1921 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     1981 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     2041 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     2101 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     2161 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     2221 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taatcgccca
     2281 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     2341 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     2401 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     2461 atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg gatcatagtc
     2521 acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt aacgaacaaa
     2581 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     2641 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     2701 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     2761 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     2821 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     2881 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     2941 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     3001 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
     3061 attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt tgccttatct
     3121 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     3181 ccaaaaacgc gtctaagaat acagtcgatt taataaaaat ttcctaattg cagtatctga
     3241 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca
     3301 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     3361 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     3421 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     3481 accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     3541 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     3601 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     3661 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     3721 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     3781 aagcagctat tcctcctgtt catatataat ctctatattg aatgggtcac aaaatgaata
     3841 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     3901 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
     3961 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
     4021 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
     4081 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
     4141 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
     4201 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
     4261 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
     4321 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
     4381 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
     4441 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
     4501 taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg ttattggcag
     4561 cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc tggaagtctt
     4621 ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga cattagtgaa
     4681 cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa ctgatttagt
     4741 ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc ttcatgccac
     4801 aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga aaggcgtttc
     4861 aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa ttcacaccag
     4921 ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga ggatcatcat
     4981 gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct ccagaatatc
     5041 ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc gcgaaagtga
     5101 cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa aatcaatgat
     5161 agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt taatattaaa
     5221 gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga gaaaattaga
     5281 atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat gcaacattac
     5341 gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa tgtttttcat
     5401 cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact cctggtgcgg
     5461 acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt ataataataa
     5521 tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg agaaagcatc
     5581 tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac cgcattggtg
     5641 tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta atcccacagg
     5701 ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg catattcact
     5761 tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg aatggtaccg
     5821 aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct ttgtctggcg
     5881 agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc caacggtaat
     5941 acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac cttcccacca
     6001 aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac aaaacttttt
     6061 tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca tgcggcaagc
     6121 aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact atccttatca
     6181 acaaatatta aaaccataat agatatttct ttatagtttt catctgattc tgaggtgata
     6241 tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag ttcaattccg
     6301 ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt gttttaaaat
     6361 tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata ttatggtatt
     6421 atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc tatttccacc
     6481 ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg gtatgaaatc
     6541 ttttcgggtt gaagtgtctc gtaatgaaat gctatgaatt catttttaga cattgttgcc
     6601 catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg tttataacat
     6661 atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt cactaccttt
     6721 gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa agagttgtca
     6781 ttaatagtta acaatgtaga aaacaagcca ttgatttgtt tgtcaagatg ctgttcatat
     6841 ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa taaatttgcg
     6901 tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt taggactagc
     6961 ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc aagggcttcc
     7021 atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa gtacaagctt
     7081 tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt tttattttcg
     7141 gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag aattttagtt
     7201 atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac agaatcctta
     7261 tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg ttgcgtaagt
     7321 ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac cacaactttt
     7381 gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg ttggagttgt
     7441 tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt ggaattaatg
     7501 ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt tttaacatta
     7561 tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat acctggggta
     7621 aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt ttttaaattg
     7681 gataattaga acaggaatgg attttcttct acacacaata aatataatgc gttatatctt
     7741 cctgatataa ttacctt
//
LOCUS       DBKEKI010000169         7715 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.171, whole genome shotgun sequence.
ACCESSION   DBKEKI010000169 DBKEKI010000000
VERSION     DBKEKI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.171"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..1704)
                     /locus_tag="ACUQPX_003741"
                     /pseudo
     CDS             complement(1..1704)
                     /locus_tag="ACUQPX_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1650..1871)
                     /locus_tag="ACUQPX_003742"
     CDS             complement(1650..1871)
                     /locus_tag="ACUQPX_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058277.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLRKAEYITARRPA
                     GQKKKHDAEHSECGTFCDEPEKTRNQDVM"
     gene            complement(1916..3852)
                     /locus_tag="ACUQPX_003743"
                     /pseudo
     CDS             complement(1916..3852)
                     /locus_tag="ACUQPX_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(3916..5577)
                     /locus_tag="ACUQPX_003744"
     CDS             complement(3916..5577)
                     /locus_tag="ACUQPX_003744"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140657 - ATP-dependent activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HHV9058278.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYGAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(5574..6137)
                     /locus_tag="ACUQPX_003745"
     CDS             complement(5574..6137)
                     /locus_tag="ACUQPX_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HHV9058279.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQQAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            complement(6426..6791)
                     /locus_tag="ACUQPX_003746"
     CDS             complement(6426..6791)
                     /locus_tag="ACUQPX_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HHV9058280.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKHHIWLR
                     DAGQCRLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     TTLPEVSGGSGRADDIIGL"
     gene            6833..7033
                     /locus_tag="ACUQPX_003747"
     CDS             6833..7033
                     /locus_tag="ACUQPX_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HHV9058281.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            complement(7165..7491)
                     /locus_tag="ACUQPX_003748"
     CDS             complement(7165..7491)
                     /locus_tag="ACUQPX_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HHV9058282.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVRFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
BASE COUNT         1691 a         2427 c         1862 g         1735 t
ORIGIN      
        1 tcaagcatcg gacgcacatc atccggggtg agacttttac cgtccgccgg ctgtggaata
       61 tttgcgaccg catcattcac cgccttctgc agtacttccg gatcgtagtc acgaccgtca
      121 cgcggaacag ggatatggct taccgcctcc ttcaccatct gctcaagcat cggacgcata
      181 tcatcgcccg tcacgcactt ctgtaatacc acagacaggg acgccagttt ttcttcaaac
      241 gtttgtgcct gcgcggctat tttcccctca aatgtgcgct gtaaatccgc cagcactgcg
      301 gaaaattctt cacccagcgc acggataatg gacagttcac gttcattcat tttttcagaa
      361 tccccctgaa catcgccttc accgcatcat gctctgtttc agtgattgcc ttattaccgt
      421 cagatgcgct gtcaggcggt tgcgctaagg ccgttttccc ggtcgacgcg aacggatctt
      481 cacgggcatc acgacgggac agcgcctcca gactgtagtt ctgctgctga agatacagtg
      541 catcacctcc cgcaaggggc ggcaggttct cacgtttacg ggcctcattg ggcgtgagaa
      601 gcgtattttt caccgactca cccagcgttt tcatgcgccg ttcgctgtcc attctcagca
      661 gcgtggtgac gtcaaactcc gtactctcgt tttcccccgt ttccagcgcc tcatccagta
      721 acagttcaat ggactcaatc agcgtctgca ggcactggga ataatactgc tgctccagcg
      781 cctccacgtt gtcactggaa ggtggctggc caacgccaat cttgtaggcc gggacacgga
      841 acaccgaaca gacaatttca gcggtcatct tcagttgttc caccgtctgc gcatccaccg
      901 gtgaaaacgt cgtggggttg tattttgccc cgttgctcaa aatggccgtt ttccccgcat
      961 tttcgccggt atacccgctg tcccagttgc tcttcagttt tttcgcattt tcttccgtaa
     1021 tactgccggg gatctcaatc accccggacg gcctgccgcc atttctgaaa aaagacgtcg
     1081 aattttcctg aatatgatgc ccctgcgtgg ccgccagccc ggcggcatac accggcggca
     1141 gccccacaag cggatgaaaa aaacagttaa accggtcgct tcccgggcag gcaccgtcac
     1201 cgcctccgtg atcccgcagt tccggtccgg cgtgatgcga tagaacacct cgccgtcatc
     1261 cgccaccaga ggttcaaccc ggctccagtc cagaatacgc agttctttga tctgcccccg
     1321 ggagttacgg attttcagca ccaccgtatt gccgtgacgc agtttggcgt tcagccacag
     1381 ttcaaaaaac tggatacgat tctgctgtgc attgggacga cgacagagac gggcaatatc
     1441 cccctgccgt ttttcacggc ggatcccctg tgtatccgtc tgcatcaagc gcagccgcat
     1501 tttggcgata tcctgggata tcagcgaaat gcaagaaaac accgcatgaa aggagaggac
     1561 actttccgga tcggctttca caccctgctg ccaggcgcca gcaaagggct cagccaccgc
     1621 ctgaaacagg ctggtccagc ccacctcttt tacatcacgt cctgatttct ggttttttcg
     1681 ggttcgtcgc aaaaagttcc acattcgcta tgctccgcat catgtttctt tttctgaccc
     1741 gccggacgtc gcgctgtgat gtactccgcc ttcctcaggc gaaccagcac ctccgcacac
     1801 ggctgtgcca catcacggat atccccggcc cgggcatcat gcgtgccctg cagatatcgg
     1861 atctttgcca taacctgtta cgggaggcgc acgcctcccg tcctccttat caaactcagc
     1921 cgccggacgc actgccgtag ttcactccgg tgatcaccgc caccgccgcg gtacggcgac
     1981 gacgccagtt gatccagcgc tccgcacgga tggccacact gcctgtctgg aacatggaaa
     2041 ccagctccac cggcgacggc gtggtactgt cgccggtcgg ctcagactgc atttccagtg
     2101 atgcctcgcg ggacatatcc actgccacgc cgccgtcatc cgccagataa atatccgggg
     2161 cattcaccag caccagctgg tcacccacgt actgggagac aatcaccggc agcccctgga
     2221 aggagccacc cagcagggtc atgtccgggt attctttctg acccagcgca tttttacgca
     2281 tggacagcgc cagggcattg gtgctggaca tcagccagac agcaccggtg ggctgcaggt
     2341 ttgctgccac aaactgtcca aacgccgcct ctgcatccgc atccgggtta ccgcttgatg
     2401 ccgtgccctt cacatcatgg gtgatggacc cggggagaca tctgccaccg ccgctttttt
     2461 cgggtccaca aattctgtat ccagacgcgc caccaccgct tccgccagcg cattacggac
     2521 cagtgcatca gcagccggac tggaaaaacg gatcaattct tccgtcagta ccgcaatggc
     2581 cgacaccttc gcatgactga aggtgatgga ttcaaaatca aacttcgtca ggggttttgc
     2641 cttaccctca cccacccagc cggcagcacc gcccgacacc tgggcgtgca cacggatatt
     2701 gaacggcacc tgacgaagtg cagggatccc gccctgacca aatcgcccga taatggtctg
     2761 aggacgcagg taatcaataa agtcctgtgc gtattcctga tattcagaca ggctgcctgc
     2821 ccactgcgga tccgtggtgg tccccgcgcc cactgccgat ttcaggacat gatgcagacg
     2881 actgtcatcc ggatactgac gacgggccac ttccagggct tcagagcgga cacctttagc
     2941 cgcagccagc gatttggcaa aacgtgcgaa accaatcccc ttatccagtt tctgctccac
     3001 acggatcacc ggcgcagaag ccaccgcggc cacattcccg ttaccggcct gtttcaccgg
     3061 ctgcgccgtg gcggccttac cggcttccag ttcacgcagg cgcttcaggt gcgcatccac
     3121 ctgacgtatt tccgctgcgg tgttgtcgta atgctcttcc tcctccacat ccagcgtgcg
     3181 cccttcctct gcggctttgg tcatgacctc ctcaagggag gctgccagtg ctgcacgctt
     3241 gttttcaaaa cttttaatct gttcgccaat attcattatg gtcttttcct tatgaaaaac
     3301 ggttgttgac tgtgccgcag cgccggcaga agatgcgatt ttcaccaccg gtttccggtt
     3361 gccggacgcg gcagaaaact ggcggtcgta agatttaatg gtccggatgg tgcattccgc
     3421 attcgcgggc acggtgacgg cagacacctc catcagctcc cagcgcagaa aatgcagtcc
     3481 gcctccgtcc agaaaggtgt attcatgagg acggaagccc acggacagcc ccttgaccag
     3541 cccggtctta atggccgccc aggcctcatc cagccgggca gccagttgcg atggcatatc
     3601 cggcacgggc ttcgccagtg ttgccgtgat ttccagccct tcgctgaccc ggcgcaccgt
     3661 acactgcccc accgggcggg aatggtcatg ctgccagaga aacgggatcg cactgccaaa
     3721 ctccgcgccc tccggctcca ggatgtcacc atcccgatcc ggagaaggcg ttgacgcaat
     3781 cccggtgatc acccgctcat cctcactgaa ggatttcacc gtcagcaggg agcaggcccg
     3841 tttaagagtc acatcagcct cctgaaaata aaaaaaccgc cgcagcggtc catgatggtt
     3901 acagggtgaa cagggttata tgaaaaaaac cgcatattct ttctttttcg gctccggatt
     3961 cagggacatc agggataccg cattgaagag cgccatcagc gggtcaattt tcccccgccc
     4021 gctggcctgt ttggtaataa ggatagcgtt accttttggc tccacccggg cattgccaac
     4081 gcaccaggcc atcagtggct gaccaccatg caccagtact ccctcagcca gtttgcgctc
     4141 ggtggttttt atggccccgc ccagcttcca gccctggctt atccccacaa caattccgtc
     4201 ggggatcccg gcttccgcca gtgaatccag aatctgcccc acacctgacg ggtcaatacc
     4261 gatatgctcc agtaactcag cctcatgaat acgacgcaca tactccgcca cttccgccgt
     4321 gtcatccccg acccgacgga caatcgtcat gtctccacag gccacaaaat cctgaaaacg
     4381 ggatgcctca ctcttccgtc tgaccaccgc ggtttcatgc gcccaggcat ggccccagcc
     4441 cagccattcg cgggtttccc tgtcacggcc aatcacgtac attcccagca gatcatccag
     4501 gcccccgccg tcaatcccca ccgtcaccac atcagcgcgc tgcaggatat cgtccaggct
     4561 gacgcgcctg ccctgctgct cccagaaatc cgcacccgcc cagcggtcag aacgcagggc
     4621 aagaccaatt tccacattgg catgttttga catgaagcca cgaaatgctt cctcaccagc
     4681 ctcccgggct ttacggtact cccggtacag aaaagcctca tccaccgaat aaccgagatt
     4741 cggattgacc atggcgaggt tttccatcag caggtgagcc ccgctttcca ccatttcagg
     4801 agggtgttca aatatcaccg gcagaaagtg cggatcatga attttgccgt cacggacatc
     4861 ccgggcgtac tgcagtttct gtctgaacac cccggctggc ggctcattcg actgggtggt
     4921 cgtatacacc acaaaccctt ccgggcggga ggcaaggccg cctatggctt cacgtaacat
     4981 gtcctccgct ttgtactgct tgccaaataa ccacagttca tcaatcagcg tccccacgga
     5041 cttgataccg gataccgtat tcggatcggc agccaccacc ttaagggtgg tgtccgtcac
     5101 cctgtgggtg atggtccgga tatgtgtctg cacctgacag aggtcatcca gatcatcgtc
     5161 ccgtcgtacc atatccctag cagggttgaa ggcgttagcc gccacctcca cggtcggggc
     5221 cagaatggtg tagcccgccg cctgccgcca gttcagtaac agcgccgtca tcatgatccc
     5281 ggcagccagc gtggacttgc tgtttttctt ggggataagg ataaaaactt ccttgatatg
     5341 gcgtacaccg gtctgcgcac cgtaggagcc aaacagggcc gccaccaggt caaacaccca
     5401 ctgtgcgcag gactccccga acgtcgggct gcccggtgca tccacaatcc gcagttgttt
     5461 aaaaatcgcc agggcatgtg cggcctgctc cggataaatc ggagccggaa taatcgacag
     5521 cccctttttc aggcgctctg cccagtccgg gcaggccgtg ctccacacag gtatcatccg
     5581 ttgccctcat tatcattatt caccaccagg cggggtggtg gtggcaccgc aaaacggtta
     5641 gccgcttttt tcgccgcgtc accttttgcc gattttttac cggcatcccc ttttttgtgg
     5701 tgcgtgaact gcgccagctt ataagccgca tccagcgcca gcctggggtc ggtattaatg
     5761 ttctccacca gaagacgccc catcgctttc accggatcgg gaagaccgtc ctccatatac
     5821 tcaataccag gagatatcac cacgggcggt ggcatctccg gatttgtttc gtccggctgt
     5881 ggtattgcag ccgcctcacg gcgacggggt ttatcctccg gctctgattt tttctgccga
     5941 taaacaggaa cctcatccac ctccaccgtc tcgcactgtt tacgggctat aaacgcaagc
     6001 acctccggat cttttgcctg ctgcgagcct ttaaccctgg ctgtcttcgc cgaataaccg
     6061 gcggcaaggg ctgacgctgt tttgtttttc ccggacatga gcgccagcgc aaattttcgt
     6121 ttttgcgttg tcagcacagc ctcctcccgg gtccagaacg cactcagccg ggtatggttc
     6181 agcccatttt tcccggcgtc tcatgccgca aatgttaact gctgcctggt taacatttgc
     6241 tgaaaaatcc tgttaacatt ttttccacgc aacaaactga ataataaaga taaaaaccgc
     6301 aaaaatgccc ggacagccag ttaacatgtt aactgccctg aaacgggaat tttttctctg
     6361 cgtgagaggg ggcgcggtgt ccggagcgat cgttttttac gccgaatgat acccccccgg
     6421 tcgggttaca gtccgatgat gtcgtccgct ctgccactac ctccggacac ctccggcagc
     6481 gtcgtgtccg gcataccacc cgccgcttca cgagcagact tttgtcgatg gcattcggta
     6541 cagagcgtcc agagattcgt ctcctcatta ccaccaccga actgaagtgc aattcggtga
     6601 tcgagttcac tgtcacagag gtcaaccaca cgaccacaga gacggcactg cccggcgtcc
     6661 ctgagccaga tatgatgctt gagggaaaca cgtgcactgc cactgacacg acgctgttca
     6721 cccttcagaa tattcacccg tcgggtattc agtgttttga ttctgctctg gagtgtacga
     6781 agctcagcca tgtaaaatcc ccgtcatatg gcaatcagta aaggaaataa atatgtcatc
     6841 gaaaaaccgt acccgcagaa ccacaacccg caatatccgt ttccccaatc acatgattga
     6901 acagatcaac atcgcccttg agcataaagg gtccggtaac ttttcagcgt gggttattga
     6961 agcctgcagg agaaggctgg caacagatgc aacgcatctg cgcccggcca gcatgacaaa
     7021 taacgagaaa tgaacgttcg gttacaggag caggtaccca ctgtcctcca acaatatttc
     7081 atcttcatat ccgacggaac aagacttacc ctgccgggat gtacagaata acaacagagt
     7141 gataattaat ttctgatgaa ataatcaggg tgcagaagga ctaaagataa acgttttctt
     7201 cacgccttta cacggcctgt ccttctcaaa tcgccatttt gccatcgcct ttacaacctg
     7261 ctcatcaaac agatggtgcg gctctgaacg aataaactca attcgggtga cagtaccatc
     7321 agcaccaata tcaaacctca catcaacccg tccctttata taatttgccg ctgcataggc
     7381 cggatattgt ggtaatgcct taaccaactg tcggggcata tctgttttat gttgcgtaca
     7441 gcccataacc agagaagaca acaaaataat taacggaaga tttcttttca ttttcattcc
     7501 ccgcacagat aagaataagt cttattctaa caatgccgcc ctgtcggtca tcaatcctct
     7561 gcttaatggc aacgacaatt atccgactta aatcacaaat cagacacatg acataacagg
     7621 gcttgcgagg taacacatcg tcctgtttct tccaccatcg caccggactg gcgactatga
     7681 ggggacaacg ccgcgctccg ttaacgcggt aaacc
//
LOCUS       DBKEKI010000170         7673 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.172, whole genome shotgun sequence.
ACCESSION   DBKEKI010000170 DBKEKI010000000
VERSION     DBKEKI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.172"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..290
                     /locus_tag="ACUQPX_003749"
     CDS             <1..290
                     /locus_tag="ACUQPX_003749"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9058283.1"
                     /translation="IGRNDPRKPISKESINQVIELLGYKGRLTGHGFRHTMSTILHEQ
                     GFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSRES"
     gene            702..1745
                     /locus_tag="ACUQPX_003750"
     CDS             702..1745
                     /locus_tag="ACUQPX_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HHV9058284.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQWEPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1889..2059)
                     /locus_tag="ACUQPX_003751"
                     /pseudo
     CDS             complement(1889..2059)
                     /locus_tag="ACUQPX_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            2260..2688
                     /locus_tag="ACUQPX_003752"
                     /pseudo
     CDS             2260..2688
                     /locus_tag="ACUQPX_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(2718..3869)
                     /locus_tag="ACUQPX_003753"
                     /pseudo
     CDS             complement(2718..3869)
                     /locus_tag="ACUQPX_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
     gene            3988..4257
                     /locus_tag="ACUQPX_003754"
                     /pseudo
     CDS             3988..4257
                     /locus_tag="ACUQPX_003754"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4331..4588
                     /locus_tag="ACUQPX_003755"
     CDS             4331..4588
                     /locus_tag="ACUQPX_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058285.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            4770..5008
                     /locus_tag="ACUQPX_003756"
                     /pseudo
     CDS             4770..5008
                     /locus_tag="ACUQPX_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            5305..5493
                     /locus_tag="ACUQPX_003757"
     CDS             5305..5493
                     /locus_tag="ACUQPX_003757"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058286.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            5821..6015
                     /locus_tag="ACUQPX_003758"
     CDS             5821..6015
                     /locus_tag="ACUQPX_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058287.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            6328..6692
                     /locus_tag="ACUQPX_003759"
                     /pseudo
     CDS             6328..6692
                     /locus_tag="ACUQPX_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611151.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(6814..7150)
                     /locus_tag="ACUQPX_003760"
                     /pseudo
     CDS             complement(6814..7150)
                     /locus_tag="ACUQPX_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2252 a         1702 c         1602 g         2117 t
ORIGIN      
        1 ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga aagcattaac caagtaattg
       61 aattacttgg atataaggga agactcacag ggcacggttt cagacacacc atgagtacaa
      121 ttctgcatga acaaggcttt aattccgcct ggattgaaat gcagttagct catgtggata
      181 aaaattccat caggggtacc tataatcatg ccctgtatct cgatggccgc cgtgaaatga
      241 tgcaatggta cgctgattac attgattcac tctccagccg ggaaagttaa gtcagaaata
      301 cttttccctg atgttttcct caattaagta ttttatcctc tggcctacaa ggctggagga
      361 tttttgttat tatattattt ttttgcgtct tcaaacttat tacaaaaaca taattaaaaa
      421 caaattgaca tttttaaatt ggcaattaca aaatacagcc ttgcagttat ttcgatacct
      481 ttagcaaagg aaaagttgat tcagatataa tttagcattt caatcatctg acatcattat
      541 aaaaaatgag attcacataa agataaaatt attgttctca aggaaagcat gaaggggctt
      601 cgtaagcaat attcattcgg tgccaagaca ggacttgata cacagaatct caacttttat
      661 gctctcagac tatatcacca catgttttac aggctcagaa aatgaacgat agattatgct
      721 ttgaagtgca cgataacaag ggttatttcg tatttccgga tacctggttt ggcccattgc
      781 tgggggagtt tgaagaagtg cttgatgcat acgacgctga tgaaatcagc gagacgagtt
      841 acatcaacaa actccgacgc cttgctcaat gggagccgga ctttattgat atacacgctc
      901 atctggcata tgcatttctg gaacagaatg ccccgcgtaa ggcactgaac gccgctctta
      961 aaggactggc ggcaggtaat cgtatcatcc cggaaagttt ctgtggtgaa ataatctgga
     1021 tgcatcccga aaaccggcct tatctgcgcg cgctttatgc agcaattctc gcaaatgtgc
     1081 acttacagcg ccatcaggat gcggttatgc tcactgataa aatactggca tacaatcctg
     1141 aagataatca gggggctcgc tggttgttgg gttcagagct gttacgcacc ggcgatcatg
     1201 agcgagcctt cagtgtactg aaagagcatg ctgatgaatt ttcaccttac tggtatgaac
     1261 tcggtctcct gcattttctg aacggggaac atgttaaggc cgccacagct tttcgccatg
     1321 gctttgccac aaacacttac atcgcagaaa tgctgtgcgg aaatctgcat ccatttccac
     1381 ttgctgtgtg gcatgacttc tccggctctc tggatacggc agaagattac tatgcaacat
     1441 actcaccatt atggggacaa tatccggaag cattgctgtt tgtaaactgg ctctataacc
     1501 attcctccgt gctgcatgaa cgatcagaaa tcattaaatg cgccgaaatg ctgattcagg
     1561 aagatgattt cgaaatatgc gaaagtattt taagacagca ggagcacctg tggaaaagaa
     1621 ttgacaagac actttctgag gaaattgtac agaaatgcag aaatatgaac ggtgaatata
     1681 tctggccctg gatattgccc ttttccgcag cagggatgaa acattcaagc atccagtatc
     1741 agtagatatt gcattagtgt aactttaaaa caagtaataa tttattatcc aataataaca
     1801 ctgtgttcct tccccaagtg attaccctgg ggttattttc tgaaagtttc ctttgcaaaa
     1861 ggatagcgtt gtaaattact ttaacgaatt actccatgaa ctcattaaag ttctttcgta
     1921 atactttatc gtcaaacacg ttatagctgg catcaaaaac cacagattta ccctgccagc
     1981 gggtaatccc ctcctctggc gttttcggtt gaactggtat ccccgtccaa cacaggcagc
     2041 cattataaac agtaaaaaat tgtattcgct gttatgaaat caatagtcct gacgtactgt
     2101 tgaatgtgct tgtgttctga acctcgtaca accagtacat ttgatatcga attataaaca
     2161 aaacattgca tccaactttt ttaatatgga caatagttgt ccatacggtg gttttatatt
     2221 cgataaatca atggatttca ttgagtcaca ggactggtaa tggatgaaaa tgctttaagg
     2281 tttgcctcat actggcgcaa ctcgcttgca gatgctgagt caggaaaggg ctgttttgaa
     2341 cggaaagacg cccaaaattt cactcactgg catgggatag cggcgggacg tcttgacgaa
     2401 gcgattgtca ataaattttt taagggagaa aaagacgatg tcgaaactgt caatgtcatc
     2461 ttacgcccaa aagtttattt ccggttactg cagcatggta aggaccgttc cgcaggcacg
     2521 cctgatattg ttacaccgat agtgacgcca gccttgctaa gccgtgaggg ttttttatat
     2581 ccgacgccag cgacctccat tcccagagac ctgcttgaac ctttgccaaa aggagcattt
     2641 tcgattggtg agattgggca gtatgacaaa tacaagacga cccatactga tcctacccac
     2701 gtaatgtgga cacggcccta agcgagatta tggttttcaa attgttccgg gctgagaccg
     2761 ccacaggcac tgtgacgacg ccagcgattg taatcacact cgatataatt aaacaccgtt
     2821 gcccgcatta tttcccggct gctaaagcgt tccccgtgga tacattccac cttcagcgaa
     2881 tgaaagaagc tttccacaca ggcattgtca taacagtagc ctttcgcact catactgcca
     2941 cgcaggttgt gtcgcttcag cattgcctga taatcccctg aacagtattg accaccacgg
     3001 tccgtatgaa caatgacgct ttccgggcgt cttctccgcc acaacgccat ttgcagtgca
     3061 tcacaggcca gttgtgctgt cattcgcggt gacatcgacc agccaatgac ggcgcgtgac
     3121 cacaggtcga tgactactgc gagatacaac cagccctcat cggtacgcaa gtacgtgatg
     3181 tcacccgccc acttctggtt tcgggccact ggcgctgaag tcctgctcca gcagattctc
     3241 caatacgggc aggccatgtg cacggtagct gaccgggctg aacttccggc tggctttcgc
     3301 ccgcagcccc tgacgacgca ggctggcggc aatggtttta atattgaact ccggcagttc
     3361 gtcagcaagg cggggagcac cgtatcgctg ctttgcctca aagaatgcct tatggacagc
     3421 ggcatcgcag gtgagccgaa actgttggcg caggctcatc tggtgacgac gcctgagcca
     3481 gacataccag ccgctgcggg caacccgaag tacacgacac atcgctttga tgctgaactc
     3541 tgcccgatga ttttcgataa agacatactt catttcaggc gcttcgcgaa gtatgtcgcg
     3601 gccttttgga ggatggacag ttcctcagcc tgctccgcca gttgtcgttt aaggcggaca
     3661 ttttcagcgg ccagttcgcc ttcgcgctct gacgaactca tttgttgctg ctgtttactg
     3721 cgccaggcat aaagctgaga ttcatacagg ctgagttcac gggctgcggc ggccacaccg
     3781 atgcgttcag cgagtttcag ggcttcgtta cgaaattcag gcgtatgttg tttacggggc
     3841 ttcttgctga ttgatactgg ttttgtcatg agtcacctct ggttgagagt ttactcactt
     3901 agtcctgtgt ccactattgg tgggtaagat caccctccat taaaactaaa acgggcgctt
     3961 tctgatatat catctgtaag cgcagtaata ccattagtag cacctctcgg cggcgcattg
     4021 attattgaat attataactg gaatgttata tttcttgtta tgatgctgtt cagtattata
     4081 ataatgttat tatcaccaaa gataactcat gcaaatgaga aagtaatatg tcaaaacagt
     4141 gcatcctttg tgaccaaaga gtttatctgc ggcactatac ttgtctcatt aatactcagt
     4201 tctctatttt gctacatatc aatatcacca tcaattttca tgcatactta tggcctgagt
     4261 acttttagtt actcaattat tttctccttc tcagttttat ttttcataat cggaaatcaa
     4321 ttatcaaaga ttgaaaaatt atccaaccta tggtttttat tacccaatag ccaattttat
     4381 cgcattacaa ataaatggtt caaaaacagg atttgccgca tctattacag gttttactca
     4441 aacaataagc gctggtacaa tcagtttcct gtcagtaacc gtaagcgaca acggtgttgg
     4501 tcccgggact actttaggtc ttggttcact aataacttca ttactgtcaa tagccgtaat
     4561 cgccattgca ggaaataata aaaagtaagt gtactctgac tgcgtatgta tctactttgt
     4621 gtaatgcagg tgtctattga aaataattct tttcgtgggc atctgttgta taaaataaaa
     4681 aattatcgta agtactccat ctgcgatgtc ttgtgaaaat tatcctgtct ggcaacaatc
     4741 gcgtccatct gtattaatgg actcgaacga tgaactctca aacaacacta gatatcaccg
     4801 gctttcgcac ttatttgcca gaatcattac gtctcagatt cgacgagcaa ttatctgtcc
     4861 gggctatcgc acagcagtta agactcagtt attcccccat tcacaatctc tttcaacgaa
     4921 ttaacgcctc aggtattgtc tgaccatgac ccgagtccct ttcattcgcc caaccgatac
     4981 catactttat gccaacagaa agaatgaatg aaccgcgcct aaaatcagcg aggacacatg
     5041 gcacaaagtg cgacatgtct cacatttaac cccagtgggg gcaacaagta taaaaccgtt
     5101 ttttgattaa gtggtcggat atgaaaaggg gaaggtttat gtgaaaaata cctgtgtttt
     5161 ctttgaaaac acacaacagt atcaggttat ttatgtttat acagtgcgtt ccaaactgac
     5221 ctgaataatt acgcaagttg caacaataaa acatttttgc cgtagtgtga ggtactccgg
     5281 taaattgctt tccaggtgca gacaatgact aacgaagaac tacaggcaat cgcattatcc
     5341 atgcttgagc gccagcgcga aaaagtacac cagcaacgag agtctttgct taacccatct
     5401 ccattcacca ggccaaaacg attggagctt gaagcattct tggacaaata cccacggcgg
     5461 cttaaatcag gcaaaaccag accaccaggc tgatagctca cagtttgttc tccgattcaa
     5521 taaaacggat aagaaccatc agcgcggtag cgtaaggcac tacagcgcat tgctttacga
     5581 gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact gcaacaccgt
     5641 atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga cccattgcga
     5701 tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa aacacgtcca
     5761 ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga ggaaaaccgg
     5821 atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca acgggagttt
     5881 gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt tccacttgaa
     5941 gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt cttagaatgc
     6001 aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg gcatcgttat
     6061 cagcgcactt gattttcttt atatccactg acgagcttgc cggggcacct ttgtttgccg
     6121 gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg acattgtaat
     6181 aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat cgttgacaat
     6241 atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct gaagcatccg
     6301 acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg aaactttgac
     6361 aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg gaaaagtggt
     6421 acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag cttgtcagaa
     6481 aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag tgcccggcca
     6541 gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc aggctatagg
     6601 caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac ggtctgaata
     6661 acgcagtgat gaagaacttc ttcagcacac tgctaaaaca catagtgatc gagcgctgat
     6721 tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa atatgaaaag
     6781 atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc gtttaatatt
     6841 cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt acgaccacag
     6901 tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata agcagttgct
     6961 tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg atttccacat
     7021 tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc tgaagttctt
     7081 tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa cccactgtac
     7141 tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact ccatacagga
     7201 taattagaga tcagtttgat tattcacaat tcatcatcag cattttctat ttctgacgaa
     7261 atcaacatga aaataaccat atatgataat tattataata acggctttaa ttggaataca
     7321 tatattacaa cgtattatat ataattggta ttctgggaac tatattctca aaatacagta
     7381 gaaacgaggt atgtttctgg tggaaaggac agttggatta aaaagtaaga attgataaaa
     7441 aaaaacgcca gcaacacata gtgccggcgg agggaatacc ccatggagaa aatgtgatgc
     7501 ctagagcatc atgaatatat aattaaaaat agttagcatt gtcaatactt tcacaaaaat
     7561 aattttcgta gtatagaaag atatttttat gcatgaccta cctgaatttg ctccgggtag
     7621 aggttataaa taaaaattga atcacgacaa acacaatatt catagtatgg cga
//
LOCUS       DBKEKI010000171         7672 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.173, whole genome shotgun sequence.
ACCESSION   DBKEKI010000171 DBKEKI010000000
VERSION     DBKEKI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.173"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            35..343
                     /locus_tag="ACUQPX_003761"
                     /pseudo
     CDS             35..343
                     /locus_tag="ACUQPX_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(374..1018)
                     /locus_tag="ACUQPX_003762"
                     /pseudo
     CDS             complement(374..1018)
                     /locus_tag="ACUQPX_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
     gene            complement(1069..1584)
                     /locus_tag="ACUQPX_003763"
     CDS             complement(1069..1584)
                     /locus_tag="ACUQPX_003763"
                     /inference="COORDINATES: protein motif:HMM:NF022866.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058288.1"
                     /translation="MLEALRSGIPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPE
                     EKALQELDTEDQRHLYLMLKEDYDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEG
                     ETVVSELEKPSKSKKVSAIKVVKPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVI
                     VDMASLFKQAG"
     gene            complement(1646..2737)
                     /locus_tag="ACUQPX_003764"
     CDS             complement(1646..2737)
                     /locus_tag="ACUQPX_003764"
                     /inference="COORDINATES: protein motif:HMM:NF017437.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHV9058289.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALAQ"
     gene            complement(2734..4842)
                     /locus_tag="ACUQPX_003765"
     CDS             complement(2734..4842)
                     /locus_tag="ACUQPX_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HHV9058290.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(4829..5650)
                     /locus_tag="ACUQPX_003766"
     CDS             complement(4829..5650)
                     /locus_tag="ACUQPX_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HHV9058291.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            5771..7141
                     /locus_tag="ACUQPX_003767"
                     /pseudo
     CDS             5771..7141
                     /locus_tag="ACUQPX_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061586541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISCR2 family transposase"
     gene            complement(7317..7619)
                     /locus_tag="ACUQPX_003768"
     CDS             complement(7317..7619)
                     /locus_tag="ACUQPX_003768"
                     /inference="COORDINATES: protein motif:HMM:NF014877.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058292.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
BASE COUNT         1992 a         1883 c         1731 g         2066 t
ORIGIN      
        1 aagatatgat gataagcagt ttcatacaac ggttatcaaa gatgttctat tatggattga
       61 acataattta gatcagtctt tactgcttga tgatgtggcg aataaagcgg gttataccaa
      121 gtggtatttt cagcggctgt tcaaaaaagt aacaggggtc acactggcta gctatattcg
      181 tgctcgtcgt ttgacgaaag cggctgttga gttgaggttg acgaaaaaaa ctatccttga
      241 gatcgcatta aaatatcaat ttgattccca acaatctttt acacgtcgat ttaagtacat
      301 ttttaaggtt acaccaagtt attatcggcg taataaatta tgggaattgg aggcaatgca
      361 ctgagagatc ccctcataat ttccccaaag cgtaaccatg tgtgaataaa ttttgagcta
      421 gtagggttgc agccacgagt aagtcttccc ttgttattgt gtagccagaa tgccgcaaaa
      481 cttccatgcc taagcgaact gttgagagta cgtttcgatt tctgactgtg ttagcctgga
      541 agtgcttgtc ccaaccttgt ttctgagcat gaacgcccgc aagccaacat gttagttgaa
      601 gcatcagggc gattagcagc atgatatcaa aacgctctga gctgctcgtt cggctatggc
      661 gtaggcctag tccgtaggca ggacttttca agtctcggaa ggtttcttca atctgcattc
      721 gcttcgaata gatattaaca agttgtttgg gtgttcgaat ttcaacaggt aagttagttg
      781 ctagaaccca tggctccttt gccgacgctg agtagatttt aggtgacggg tggtgacaat
      841 gagtccgtgt cgagcgctga ttttttcggc ctttagagcg agatttatac aatagaattt
      901 ggcatgagat tggattgctt ttagtcagcc tcttatagcc taaagtcttt gagtgactag
      961 atgacatatc atgtaagttg ctgataggtt tccagttttc cgctcctagg tctgcatatg
     1021 tgctataaat cagctcattc gagtacggaa caggaaagtt tctcatggct aacctgcttg
     1081 tttaaataag ctcgccatat ccactattac ccctttccct tttaaccgtt catgcatggt
     1141 tttttcaggt tggcgttgtg aatagatata acgtaaatcc gtatcaggca agctatccca
     1201 gtcgctgggc ttgactacct ttatagccga aaccttttta ctcttggagg gcttttctag
     1261 ttctgacact accgtttcgc cttccatcaa ccactgcaaa acaagaggca gtaacttttg
     1321 tcttgtcatc gttggattct ggctaaacgc ttttttaata gtgggaatta acaggcttga
     1381 atcgtaatcc tctttcagca tcagatataa atgacgctga tcttcggtat ctaactcttg
     1441 aagggctttt tcttctggtg tttgttcttg tatcgctgcg atatctagct gaagttggat
     1501 taaccgttta tcaatctcgg gaacgactag atcagaataa cgagcaatgc gttctgggat
     1561 acccgagcgt aatgcctcta gcatggggtg cacaggcttt aactcatctt gatacacttg
     1621 ccgcaataaa ccagcggtaa tacgctcatt gcgctaaagc tagcgcacgg agctgagcga
     1681 gtacaaaaag ttttactaca atgtccatca ctccttggct tagctcatac cacacatcac
     1741 ggacctcatc cgataacagc gcatctttgc gttgtaaaag ctgtaattgc caaagattat
     1801 ccgtaaaagc gatccactct tgattgggct ttccacgttg cgtttgttgt ataggatccc
     1861 agaatatagc tccaaaccct gcccctctac gtgcagaccg caaatcagcc tcaaaaatct
     1921 ctcgtgcttt aggggtacca atcaacatca ctggtacgcc aataatattc accatcgtca
     1981 caaaaaagtt cagcatctct tgagatccac ccgaacgaga gcggcttaaa tgctgaattt
     2041 catcaataac caacaaccct aaagcatgtg cattggctat ttgcgacatc aaagccaaca
     2101 tggtttctat accatgacgt tttaagccat aacgacgctc atagttcgag cccaaggctc
     2161 gatccaacgc tctgaaaaaa ttcaagcaga tttcttttag cgaaccatta tgcgagcagt
     2221 ctattttcaa atacaccacc tgctctacat tgagttcacg atggtaaatc acctgaggat
     2281 acgtggctag aatacgatga agagaggtcg tcttcccact accagaacaa ccaattaaca
     2341 ataagctttg tgccgtagat cgtgcctcct caaagcgaaa tgtctccaac tctcccgttt
     2401 gaacacgctc ataaccattt tgtaaatgct tttgtaaatc tcctgtttta ggatttctgc
     2461 ctacgtagcc acctcgaatc atgaccgaaa tacgctcact tagtagcaaa tgcgtaccta
     2521 atggctgaaa atagtcatct ggaatacgac aaatggtatg agctctgata acacgggact
     2581 tttgcaagtc agaggaagta agctgtaaag aggatttcag tgatgcagca ctattcactg
     2641 actcttgtaa tggtggtaag gcctcgataa aaggattatc acgataagcc tctacccccg
     2701 tatcacgata aactgcttga atccgggtag cactcatgac tcatcctttt ctggtggatc
     2761 ctgaaataat tcaggcacgt atgtgggtag gctgtaatct tcttgatcat ccgcttccac
     2821 tgcgttgaaa ggaataactt tagcctcatc acctgaagag cttggcttca aatgctccgc
     2881 acgtttttta cgctccgagg tcacggcttc ttttttatta gttttaatct gcttaatgcg
     2941 tgttgatttg ggctcagtag tactgggcgt taacttattc gctttctgaa tggtttgctg
     3001 aataaacgcc tcaagctccc tgcgtttagt taactcatcc tgcttcgcat tggctttatt
     3061 gtgtttttct tgtgcttgta tatcccaaac ctcccaaaat gagagacctt taaactgccg
     3121 actacgttcc gtcagattac agcgccaaaa tacacggctg ccaacttgcg gaaacaaata
     3181 aatcgtatca accagcactg ggtcataagc cgcttctaaa tgttgaggtc tagctatatc
     3241 agtgctccgc tgcaaccaac cctcacgcag aatctctgac cccgagtaat acaaacccca
     3301 caaattaacg ccaaatgaag aaatagagac ctttcggcga ggcagtaacg ctactcgcaa
     3361 ctgctcttgc tccacagccc ttaaactacc tgtacgatgc tgcatacccc attgccatag
     3421 ctggacagga atagacggta aatctgtagg aaaatcagca tctcgatcgt atttatccat
     3481 caccagatgg ttatttctga ataagatcgt acgcaaaata atttgtgtga actcaaatac
     3541 cgacagagat gcatctaacc tatagtctgt ttcaccatgg cttttgatcc gactgccctc
     3601 tacaatgcca ggtgcaaagg acttaaactc ggcttgtagt gttctaaaag tgctttccac
     3661 tatgccttta gcatcgccac gtctaggtgg agcactttcc actcgcacat taaaactaga
     3721 aactaaggct tcgacctgat gactcattaa ttcgccacgg tccgctagca acacatctgg
     3781 caaacctaca cacggccagt ctgagctact aatctcaata tcatgctggg cacaaatggc
     3841 cgttttgtca gagcaagcat ttacaaaagc ctgcatcgcc accacataag acggattttc
     3901 aaagccgata taaaagcccg tgatcatccg actaaacaca tcaatcacaa tgtaaagcgt
     3961 tggtcttcct atgatttttt ggcgatcatg atgatccact aaataaatat cagcaatcgt
     4021 ggcatcaatc tcataacgac tcccagggcc taacgcctga gaagtggctg tactacttaa
     4081 gggtcgtacg tcttttttat atacccctgc tttaactcta gactttaagc gctgagcttt
     4141 agggtattct cgatcataaa aataacgaaa ctgacgtagt gttgggtaat cctcttgggg
     4201 aatgcgagga aaatactgag caaacaagtc cacaaatcgt ctataggcaa cggtggtctt
     4261 tgtacctttt tgatttaaca ggtgcttttc tatggtcaac ctaaaaaggc gttcaatctc
     4321 gggcgttacc ttggttcctt cacccttacc atattctctg gctcggccaa tctttgctgt
     4381 tcctgtcgct gaacgtcttt cccctggtgc accgctgttt ttgtagtcag gaattaatgc
     4441 attaggcgtt tgaccacgct gccagtaacg gcgtaacaac ttataaactg tagccttagt
     4501 aaccttatgt tcttggacca catgctcaac gagttcgctt ctgactttag ggtcgaaacg
     4561 atccttacta ttaataatag gaagaatttt tcgataatcc tcctcgcgct tctggaagct
     4621 gacagaatct acagacggct cttctaaatc aagatgtaca taagggtcat cagttctaac
     4681 taagcggcct tcatctaaat actgcattag caacaactca gccctagctt gtggaactcc
     4741 tttatcagcg cttatttgca tccagacaac ttggccatcc tctatagcca aaatgcgata
     4801 cggatcatta tcaaatagca caacctcatt aatttgccac atagcgcaac tcctccatat
     4861 tcactacttg gctaatacag agatctgcac acttatttgc cctgaaagac ttataaatat
     4921 tgaacttaat aaaaccattt gcggttaagg ctcgtatctc actcaatgtt ttgcctaact
     4981 ccaaatcata agcaatatca acctgcttac agacattgat aatgttttca tctccttttt
     5041 cttgcaggat atgggccaat ggggatagtt gtgctaaaag ctccgcagaa acttcttctg
     5101 ttttcactga ataaagccat tcaatatttt cttttactac gggatttatt tctttatcag
     5161 taaaaatgaa ccaaggaatt tgcttttgct gccaatagcg acgctctagt tctagttttt
     5221 ctaaggtacg ctcgtcttgt aaggctgctg caggtttgac ttgaatagca aactgctcaa
     5281 aaggaccatc tttgcagtcc actaaaaaat cagtagacat aacctgatct acaccacgaa
     5341 taacaggatg cttaatacca ctatctattg caatctgcct ggtatcacta ggtaataagg
     5401 ggaactgctc gcgtatatct agcacgctgc tctcccactc aagactgaga aaaacagcaa
     5461 gctctaagtc agatagcaaa tgatggactc gtcccgtctt atgagaataa atacggtggg
     5521 aacgacctga agaaggaact tcttgtactg ttagccatgg aatatagtct ttaccatgcc
     5581 cttggccacg cccctcttta atacgacggg caatttgcac ttcagaaaat gaagagtttg
     5641 ctttagccat aacaaaagtc cagtatgctt tttcacagca taactggact gatttcagtt
     5701 tacaactatt ctgtctagtt taagacttta ttgtcatagt ttagatctat tttgttcagt
     5761 ttaagacttt attgtccgcc cacagggaaa ggaattgccg ggctatgtgc aacgggaatt
     5821 tgaagaattt ctccaatgcg ggcggctgga gcatggcttt ctacgggttc gctgcgagtc
     5881 ttgccacgcc gagcacctgg tcgctttcag ctgtaagcgt cgcggtttct gcccgagctg
     5941 tggggcgcgg cggatggccg aaagtgccgc cttgctggtt gatgaagtac tgcctgaaca
     6001 acccatgcgt cagtgggtgt tgagcttccc gtttcagctg cgtttcctgt ttgccagccg
     6061 gcccgagatc atggggtggg tgctgggcat cgtttaccgc gtcattgcca cgcacctggt
     6121 caagaaagcg ggccataccc accaagtggc caagacgggc gcggtcaccc tgatccagcg
     6181 ttttggatcg gcgctcaatc tgaatgttca cttccacatg ctgtttctcg acggtgtgta
     6241 tgtcgagcaa tcccacggct cagcgcgttt ccgctgggtc aaggcgccga ccagcccaga
     6301 gctcacccag ctgacgcaca ccatcgccca ccgggtgggt cgctatctgg aacggcaagg
     6361 cctgctggaa cgggatgtcg aaaacagcta tctggcctcg gatgcggtgg atgacgaccc
     6421 gatgacaccc ctgctggggc actcgatcac ttaccgtatc gctgtcggtt cacaggcggg
     6481 gcgaaaggtg ttcactttgc aaactctgcc gaccagtggt gatccgttcg gtgacgggat
     6541 tggcaaggta gccgggtcca gcctgcacgc cggcgtggcg gccagggccg atgaacgcaa
     6601 gaagctcgaa cggctgtgcc ggtacatcag ccgcccggcg gtatccgaga agcggctgtc
     6661 gttaacacga ggcggcaacg tgcgctacca gctcaagacg ccgtaccggg acggcaccac
     6721 gcacgtcatt ttcgaaccat tggatttcat tgcaaggctg gccgccctgg taccgaagcc
     6781 cagagtcaac ctaacccgct tccacggggt gttcgcaccc aacagtcggc accgggcgtt
     6841 ggtcacgccg gcaaaacggg gcaggggcaa caaggtcagg gtggctgatg aaccggcaac
     6901 accagcacaa cggcgagcgt cgatgacatg ggcgcaacgg ctcaagcgtg ttttcaatat
     6961 cgacatcgag acctgcagcg gctgcggcgg cgccatgaaa gtcatcgcct gcattgaaga
     7021 ccctatagtg atcaagcaga tccttgatca cctgaagcac aaagccgaaa ccagcgggac
     7081 cagggcgtta cccgaaagcc gggcgccacc ggctgagctg ctcctgggtc tgtttgactg
     7141 acgagcctga aggccaacga taccaatcaa aatgctgcgt tcacagcgcc gcggcaggga
     7201 tccgccgtgc tggttgtcgg aaaaggagcc gctagtggga aagaggaggg taaattttca
     7261 gcgttgctgg ctccccgtca gccggattgg gttgcatcgc aggggtgtcg aaagagtcaa
     7321 ctgcggtcca aagctgttgg acttgggtga aaagggcgtt tattcttcct atacgttgtc
     7381 ggcagcgggc caaaaaggaa tacgtccatg cccatcgagg tgaaaccggc tgtgagcgcg
     7441 ggttcaagca tatagcccga caggcgcgta tccttgccga tcacgacacg atggcggtgg
     7501 tcaccgcgac gaaagacacg gccagccgcc atgccgacgc gcaaggcggt ttccgccgtc
     7561 atcgcgcctt cgttggcttt gccacgaata ccgtctgtgc cgaaatattt gcgcaccata
     7621 aggtcgatta tcctgtcgtc gggtcgccct caaaggggac atgcctgctg aa
//
LOCUS       DBKEKI010000172         7526 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.174, whole genome shotgun sequence.
ACCESSION   DBKEKI010000172 DBKEKI010000000
VERSION     DBKEKI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.174"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            556..1326
                     /locus_tag="ACUQPX_003769"
     CDS             556..1326
                     /locus_tag="ACUQPX_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine ThrE exporter family protein"
                     /protein_id="HHV9058293.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1317..1790
                     /locus_tag="ACUQPX_003770"
     CDS             1317..1790
                     /locus_tag="ACUQPX_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HHV9058294.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1897..2436
                     /gene="dnaT"
                     /locus_tag="ACUQPX_003771"
     CDS             1897..2436
                     /gene="dnaT"
                     /locus_tag="ACUQPX_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HHV9058295.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2439..3176
                     /gene="dnaC"
                     /locus_tag="ACUQPX_003772"
     CDS             2439..3176
                     /gene="dnaC"
                     /locus_tag="ACUQPX_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HHV9058296.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3225..3719
                     /gene="yjjA"
                     /locus_tag="ACUQPX_003773"
     CDS             3225..3719
                     /gene="yjjA"
                     /locus_tag="ACUQPX_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HHV9058297.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3973..6264
                     /gene="opgB"
                     /locus_tag="ACUQPX_003774"
     CDS             3973..6264
                     /gene="opgB"
                     /locus_tag="ACUQPX_003774"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HHV9058298.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6402..7424)
                     /gene="lgoD"
                     /locus_tag="ACUQPX_003775"
     CDS             complement(6402..7424)
                     /gene="lgoD"
                     /locus_tag="ACUQPX_003775"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HHV9058299.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
BASE COUNT         1913 a         1949 c         1833 g         1831 t
ORIGIN      
        1 tttccagcaa attaatagat taagcgaata aattatcctt agcattcact ttcatatcag
       61 catactcctg ctgcacaaca actccactgc atgtgtcaga aggttagcat caaatgataa
      121 cctggtgaaa tagcctggag acatttaaaa tatctacatt aagttgttag aaatacttaa
      181 tgaaaaacag aaatctcatt catttaattt ataattaaat gatatctctt cccgcagaat
      241 gcgggaatca cacaatcaat atttattcaa taaaatctga tgattttaac ttgttaacac
      301 taaaaaacgc gcccagctat ctattatcct gacccgtaaa ggctaaacaa taaattcatt
      361 atagtgataa tattcatgtg atagtttgtt tgcggcgaga gataattcgc tttttatcac
      421 cgagcttgtc ccaataacca ggattaatca gcgcaatttt gatcttaacc taccgcaaaa
      481 caaccgtgct tcatgataca aatggattct cattatctga acaatacgca gcacgtctat
      541 gacaaagggc gagttatgca aactgagcaa cagcgagccg taacgcggct ttgtatccag
      601 tgtggattat ttcttttgca acatggtgcg gaaagcgcgt tggttgatga gctttcctca
      661 cgactgggtc gggcgctggg aatggacagc gtcgaaagtt ctatctcttc gaacgccata
      721 gtgctgacaa ctattaaaga tgggcaatgc ctgacatcga cacgtaaaaa tcacgatcgc
      781 ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattcttgc ggagcatcat
      841 ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcaaccatt acgttaccca
      901 agatggctgg tagccttaat ggttggcctt tcttgcgcct gtttctgtaa actcaataac
      961 ggtggctggg atggtgccgt catcaccttc tttgccagta cgaccgcgat gtatatccgc
     1021 cagctgctgg cacaacgtca tcttcatcca cagatcaact tttgccttac cgctttcgcc
     1081 gccaccacca tttccggatt gcttttgcaa ctccccactt tcagcaatac ccccaccatt
     1141 gcgatggccg ccagcgttct gctgctagtg ccgggctttc cgttgattaa tgccgtcgcc
     1201 gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc cagtctgctg
     1261 acactggcta cctgcgtcgg cgtagtgatg gcactgacga tttgggggct acgcggatgg
     1321 gtgtgatcga atttctgtta gcgttggcgc aggatatgat cctcgccgcc attcctgcgg
     1381 tcggctttgc gatggtgttc aacgttcccg tgcgggcgtt acgctggtgt gcgctgcttg
     1441 gctcgatagg tcatggttcc cgaatgatct tgatgaccag cgggttgaat attgagtggt
     1501 caacctttat ggcttctatg ctggtcggta ccattggtat tcaatggtcg cgctggtatc
     1561 tggcgcatcc gaaagtgttt accgtggcgg ccgttatccc tatgttcccg ggcatatcgg
     1621 cttataccgc aatgatttcg gcggtaaaaa tcagccagtt aggttacagc gaaccgttga
     1681 tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgcg ttatccatcg
     1741 gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa aattgcctcg
     1801 cttaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata aaagttatct
     1861 cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt tttgaccccg
     1921 gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc aaaagctgaa
     1981 ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt cacgcctgca
     2041 cgcctggctg aactgctggc gctggaagaa aagctagcgc gtccgggaag cgatgtcgct
     2101 ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc catggggaaa
     2161 ttcgccatgt atccggactg gcaacccgat gccgatttta tccgcctggc ggcgctatgg
     2221 ggcgtggcgc taagagagcc ggtgaccacc gaagaactgg cctcattcat tgcctactgg
     2281 caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc gcgcagcctg
     2341 caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac ggtcagtgaa
     2401 cctgacagcc aaattccacc aggattcaga gggtaacgat gaaaaacgtt ggcgacctga
     2461 tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa acgggtgaag
     2521 agctactggc ctggcagaaa gaacaagggg cgatccgctc cgccgctctc gaacgtgaaa
     2581 atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcgtcca ctgcatcaga
     2641 actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg ttaagcaaag
     2701 cgcgccagta tgtcgaagag tttgacggca acatcgccag cttcatcttt tctggtaagc
     2761 caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg ctacgcggta
     2821 aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat accttcagga
     2881 atagcggtac cagcgaagaa caactgctta acgatctgag caacgttgat ctgctggtga
     2941 tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc aaccagatcg
     3001 tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt aatatggaag
     3061 agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt aacagtttat
     3121 gggtgatctt caactgggat agctaccgta gccgggtaac gggtaaagag tattaatccc
     3181 cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg aaaactatta
     3241 aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg gtgcatgctg
     3301 cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa aacagcacga
     3361 cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc ggaaaccagg
     3421 ccttaagcgc agataacatg aacaatgccg caggcattct gcaatactgc gcgaagcaaa
     3481 agctggcttc ggtaaccgat gccgaaaaca tcaagaacca ggtgctggaa aagctgggcc
     3541 tgaacagtga agagcaaaaa gaagacacca actatctgga cggtattcag ggtttgctga
     3601 aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg aacgactccg ctggcagaaa
     3661 aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc atttcctgaa
     3721 ctgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg gctctctcct gcccttttcc
     3781 ctgctcctgg acggttttcc ccctgttaca cgggaagcga ccgcgatcaa aaaagtctgt
     3841 ttataaacta attcctaaca acatcgcatt tttttgacac tagaattgca gcaatatggc
     3901 gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg gctaaacggc ccggtttagt
     3961 gaggatcgcc cgttgtcaga actactctct ttcgcccttt ttctcgcctc tgtgctgatt
     4021 tacgcatgga aagcgggacg taacacctgg tggtttgcag ccacgttaac ggtgctgggg
     4081 ctatttgtcg ttttaaatat caccctgttt gccagcgact attttactgg cgatggtatt
     4141 aacgacgcgg ttctctatac cttaaccaac agcctgaccg gtgctggcgt cagcaaatac
     4201 atcctgccgg gtatcggcat tgtgctgggg ctgacggcgg tgttcggtgc gctgggctgg
     4261 atcctgcgcc gtcgtcgtca tcatccgcac cattttggtt acagcctgct ggcgctctta
     4321 ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc agataacgga actggtgaaa
     4381 tcccagtcac gcgatggcga cccggacttt gcggcttatt ataaagagcc gtcgaaaact
     4441 atccctgacc cgaaactcaa cctggtttat atctacggcg aaagtctcga gcggacctat
     4501 tttgataacg aagctttccc ggatctcacg cctgaactgg gcgcgttgaa aaatgaaggc
     4561 ctggatttca gccacacgca gcagctgcca ggaacggatt acacgattgc gggcatggtg
     4621 gcttctcagt gcggcatacc gctgttcgcc ccctttgaag gcaacgcctc cgcctctgtc
     4681 tccagcttct tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc gggttatcag
     4741 aactatttcg tgcagggcgc aaatctgcgt tttgccggta aagatgtgtt cctgaaatcg
     4801 cacggcttcg accacttata cggctcagaa gagctgaaaa gcgtggtagc cgacccgcac
     4861 tatcgcaacg actggggatt ctacgacgat accgttctcg atgaagcgtg gaaaaagttt
     4921 gaagagcttt cccgctcagg tcagcgattc tcactgttta ccctgacagt cgatacccat
     4981 cacccggatg gttttatctc gcgcacctgt aaccgcaaaa aatatgattt tgacggtaag
     5041 ccgaatcagt cattcagcgc ggtaagttgc agccaggaga atatcgcgac gtttatcaac
     5101 aaaatcaaag cgtcaccgtg gtttaaagat actgtcatcg tcgtctcttc tgaccattta
     5161 gcgatgaaca acacggcgtg gaaatacctc aataagcagg atcgcaataa cctgtttttt
     5221 gtcattcgtg gcgacaagcc gcagcaaaag acgctggcag tgaagcgtaa cacgatggat
     5281 aatggcgcga cggtgctgga cattctcggt ggcgataact atctcggact tggtcgtagc
     5341 agtttatccg ggcagtcgat gtcggaaatc ttcctcaata tcaaagagaa aacattggcg
     5401 tggaagccag atatcatccg cctgtggaaa ttccctaaag agatgaaaga gttcaccatc
     5461 gaccagcaga aaaacatgat tgccttctcg ggtagccatt tccgtttgcc gctgctgttg
     5521 cgggtttcag acaaacgcgt ggaaccgctg ccggaaagcg aatactcagc accgctgcgt
     5581 ttccagctgg ccgatttcgc tccacgcgac aatttcgtct gggttgacag ttgctacaag
     5641 atggcacaac tctgggctcc ggaactggca ctttccaccg actggtgtgt ctcgcagggg
     5701 cagcttggcg gtcagcaaat tgttcagcat gttgacaaaa caacatggaa gagcaagacg
     5761 gcatttaaag atacggtcat cgacatggcg cgttacaaag gcaatgtcga tacgctgaag
     5821 attgttgata acgatattcg ctacaaagcc gacagtttca tctttaacgt cgccggtgcg
     5881 ccagaagagg tgaaacagtt tagcgggatt tcccgtccgg agtcgtgggg ccgctggtcc
     5941 aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc atccgctgcc gaagaaattt
     6001 gacctggtga ttaccgccaa agcatacggc aataacgcca gccgtcctat tccggtacgc
     6061 gtaggcaatg aagaacaaac tcttgtgctg ggcaatgaag tgaccaccac cacactgcat
     6121 ttcgataacc caaccgatgc cgacacactg gtaattgtgc cgccggaacc tgtctcaacc
     6181 aacgaaggga atatcctcgg acactcgccg cgtaagctcg ggatcggcat ggtggaaatt
     6241 aaagtggtag aacgtgaagg gtaattcctg actgatgtgt aggtcgcata gggtgcgacc
     6301 tacacgcctt ctccggctta caacacatta ttaagaaaca aaaaaaacag cgcccaacac
     6361 ttccccggac gctgcatggc aactttaata atctcgcctt atcagaaagt aattacaccc
     6421 ttaatcaact cacgattgtt aatcacatcg cgctcgtagg tttctgccag cgtggcgaac
     6481 ggatagcgat gggttaacat catgtcggca gtgattttcc cttccgccat cagtcgaccg
     6541 actttagcga aatcttccgg cgtggcgttg cggctgccca tcatcgtcgt ttctttttta
     6601 tggaattccg gatcggagaa ctgcaactca cctttaaaca gaccgacaaa taccaccgtg
     6661 ccgccgtgac gaatcagatt cacggtgtta ttcatcgcat gttgattacc tgtcgcgtcg
     6721 atcactttct gcgccagcga accaccaaac tgcgcccgca gctgcgcgtc aaaatcttcg
     6781 gctgacgggt ccagcacggg caactccaga cgcgttgcca catgttcacg gcgcgccgga
     6841 ctggtatccg ccaccaccac ctgtgcgcca tcggctttag cgattgccgc cgcgcccaga
     6901 ccgattggcc ccgccccgac caccagcacc tgctcgccgg gagcaatggc tgcgcgacgc
     6961 accgcatgag cgctaatagc gaaaggttca atcaatgcgg ccgcctgcgg gtcaataccg
     7021 tctgcgggca aaatgttcgc caccggcacg ctcaaatact cactaaaacc gccatcctga
     7081 tgcacgccaa tgactgaaat tttttcacag cagttggtac gcccgctttt acacgccggg
     7141 cattgctgac aggcaacata agggatcaca gcaacttgct gaccattttt aagatcagca
     7201 atatttttac ccagcccaac aatctcccca catatttcat ggcctaaaac ccgtggataa
     7261 ctaaaaaatg gttgatttcc accccaggca tgaatatcgg taccgcaaat cccgacagac
     7321 ttaattttta ttaatgcttc attgtcaccc ggaataggta tctcgcgttg tttccagact
     7381 aattctttcg gctgctggca aattaaaaca ttcatcgtag acatgattcg gctctccatt
     7441 ttttgttgcc ttagatatca atgaaaatga aaaatggcag cggaacggaa aatctgtttt
     7501 gtgaaaacac acgcataaaa atgttt
//
LOCUS       DBKEKI010000173         7474 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.175, whole genome shotgun sequence.
ACCESSION   DBKEKI010000173 DBKEKI010000000
VERSION     DBKEKI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.175"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..333
                     /locus_tag="ACUQPX_003776"
     CDS             <1..333
                     /locus_tag="ACUQPX_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HHV9058300.1"
                     /translation="TLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYLNSLRLVAAN
                     SYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYDEENAHLYER
                     LLTQQGDE"
     gene            275..1756
                     /gene="clsC"
                     /locus_tag="ACUQPX_003777"
     CDS             275..1756
                     /gene="clsC"
                     /locus_tag="ACUQPX_003777"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HHV9058301.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(1764..2921)
                     /gene="mdoC"
                     /locus_tag="ACUQPX_003778"
     CDS             complement(1764..2921)
                     /gene="mdoC"
                     /locus_tag="ACUQPX_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HHV9058302.1"
                     /translation="MNPVPAQREYFLDSIRTWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPRLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3315..4850
                     /gene="mdoG"
                     /locus_tag="ACUQPX_003779"
     CDS             3315..4850
                     /gene="mdoG"
                     /locus_tag="ACUQPX_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HHV9058303.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KQHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4843..7386
                     /gene="mdoH"
                     /locus_tag="ACUQPX_003780"
     CDS             4843..7386
                     /gene="mdoH"
                     /locus_tag="ACUQPX_003780"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HHV9058304.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMLEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1846 a         1791 c         1967 g         1870 t
ORIGIN      
        1 actcttgcag gcgatcttcc cgctaaagcc gtagtacaca ccgtcgggcc agtctggcgt
       61 ggtggtgaac aaaacgaaga ccagcttttg caggatgcct atctgaatag cctacgactg
      121 gtggcggcaa acagctatac gtcagtggct tttcctgcaa tcagtactgg ggtttatggt
      181 taccctcgtg cggcagcggc tgaaatcgca gttaaaaccg tttcagaatt tattacccgt
      241 cacgctttac ccgaacaggt atactttgtc tgttatgatg aagaaaacgc ccacctctac
      301 gaaagactcc ttacccaaca aggagatgaa tgatttgccc cggctggcga gcgcggtgct
      361 gccactgtgt tcgcaacatc ccggtcagtg tggtcttttc cctctggaga aaagtctgga
      421 tgcgtttgcc gcccggtatc gtctggccga aatggcagag catacgctgg atgttcagta
      481 ttacatctgg caggacgata tgtcggggcg gttactgttt tccgccctgt tagccgcagc
      541 aaagcgtggc gttcgcgtcc gtttgttgct ggacgacaac aatacgcccg gacttgacga
      601 cattttacgc ttgcttgaca gtcatccacg cattgaagtc cggcttttta atcctttctc
      661 gtttcgcttg ctacgtccgc ttggatatat caccgacttt tcccgtctca atcgccggat
      721 gcacaataaa agtttcactg tcgatggcgt ggttacccta gtgggaggac gaaatattgg
      781 tgatgcctat tttggtgcag gggaagagcc acttttttcg gatttagatg tcatggcaat
      841 aggccccgtg gtagaggacg ttgccgatga tttcgcccgc tactggtatt gcaaatcggt
      901 ttcaccctta cagcaggtgc tggatgtccc ggaaggtgaa atggcggatc gcatcgagtt
      961 acccgcctcc tggcataacg atgccatgac gcatcgttat ttacgcaaaa tggaatccag
     1021 tccatttata aatcatctgg ttgatggaac gttgccgctt atctgggcga agacacgttt
     1081 attaagtgat gatccggcga aaggggaggg caaggcaaaa cggcattcac tgttaccgca
     1141 gcgtctgttc gatatcatgg gctcacccag tgaacgcatc gatattatct cttcctattt
     1201 tgtaccgaca cgcgcaggtg tggcgcaact cttacggatg gtgagaaaag gggtaaagat
     1261 tgcgatccta accaattctc ttgccgctaa cgatgttgct gtcgtccatg ccggatacgc
     1321 gcgctggcgc aaaaaattgc tccgctatgg cgtggaatta tatgaactca agccgacgcg
     1381 tgaacaaagc agtacgttac acgatcgcgg cataaccggt aattccggtg ccagcctgca
     1441 tgctaaaact tttagcatcg atggtaaaac ggtgtttatc ggctctttca atttcgatcc
     1501 gcgttcaaca ttgctcaata ctgaaatggg cttcgtgata gagagcgaaa cgctggcaca
     1561 gttaattgat aaacgcttta ttcagagcca gtacgatgcg gcctggcagc tccgtctgga
     1621 caggtgggga cggatcaact gggttgatcg tcatgcaaag aaagaggtta ttctcaaaaa
     1681 agaacccgcc accagtttct ggaaacgggt tatggtcaga ctggcgtcga tattgcccgt
     1741 ggaatggtta ttgtaaatgt ggcttaacgg gctggtgctt tatcgttctc acgcttaaca
     1801 accggtttac cagagaacaa aaacttcagt aacgggatgc gtaaatgaat ttcatacaga
     1861 attatcgcaa tccctactac gaatatcagg ccacagagaa aaccaagcca gttggaggtg
     1921 atgtgcggtg taatgtatgc gccgaaaaac agcgttaacg ggtggtgaac cagatagata
     1981 aacagcgatg cgttaacaaa ataagtcacc cgcgctgact ggaagttaag caaacggtgg
     2041 ccaaaggaga agaccacatt caccatccac aggccgagga ccatggtgat caccgactcg
     2101 gtttcgtaca tccaggcatc gccactgcca tagcgctggt tgagtaaata agcgacaaac
     2161 gccaatgctg cagcgagggt acagccacga gacggcgtgg taaacaaggc tttaagatga
     2221 gggaaaatga aagccagtgc gccgaggata aagaacggta aataaaatag cgtttgcatg
     2281 acaataaaat tgaacatgcc attactcaga atgggtggat acacaataaa aatcgttctt
     2341 cttattaccg catagccgat gccgaggcat aaaaaaatca ccgatagttt taccatcgag
     2401 aattttttat tcgttttatc agaattttct aaattatttc tgatgcgctt aaatatccat
     2461 acacacagcg tcgtcatgac tactaacacc agtaaaaacc acaggtgtga tattaattcc
     2521 caggccaacg tattatattt gtcatacaat gatagcctag gccaactttc cgctttccct
     2581 ttgacatatt gcagcataat aaattgcggt aatgtcagta gggggatggc tgttaacatc
     2641 gggataccta cacgttcgac acgtactttc caccattttt tcaagggata gcgtaaaaaa
     2701 agcatgtagg aaaagtatcc ggatataacg aaaaatacct gcatgcggaa cgagtggatg
     2761 aagtcattaa aaagggtcag ccacaatgac ggttcggcgc tattcacatg ccatgtatgg
     2821 ctcgaataga ttaaagaaat atgaaaaggg atccctaaca acatcagcca ggtgcggatg
     2881 gagtcgagga aatattcacg ttgcgcgggt actggattca tatatggtta actaatctcg
     2941 gatttttcgt cttatccctg tcgggttatg cctttaggct tgttgccata gcgacaccga
     3001 cctgaccgcg ccaggcgcag gcttcaaggt ttttatgcat agcatcatcg ctaccactaa
     3061 ccagaatgga agcgtctgta agacggttga taaataaatt tgctggcaaa ccctacacga
     3121 agtcgatgct tctgtcttta ggagaagcac ggaaagtgaa aacggttgca atcaggtgct
     3181 taatccatga gccagcgtgc tgaacgatac cgggattcag ttgtcggaat ggctggttat
     3241 ccattaaaat agatcggatc gatataagca cacaaagggg gaagtgctta ctaattatga
     3301 aacataaact acaaatgatg aaaatgcgtt ggttgagtgc tgcagtaatg ttaaccctgt
     3361 atacatcttc aagctgggct ttcagtattg atgatgtcgc aaagcaagct caatccttag
     3421 ccgggaaagg ctatgaggcg cctaaaagca acttgccctc cgttttccgc gacatgaaat
     3481 acgcggacta tcagcagatc cagtttaatc atgacaaagc gtactggaac aatctgaaga
     3541 ccccattcaa actcgagttc taccatcagg gtatgtactt cgataccccg gtcaaaataa
     3601 atgaagtgac tgccaccgca gtcaaacgaa tcaaatacag cccggattat ttcactttcg
     3661 gcgatgttca gcatgacaaa gacacggtaa aagaccttgg ttttgccggt tttaaagtgc
     3721 tttacccgat caacagcaaa gataaaaacg atgaaatcgt cagcatgctc ggggccagct
     3781 atttccgcgt gattggtgca ggtcaggttt atggcctttc tgcccgcggc ctagcaattg
     3841 ataccgcttt gccatcgggt gaagaatttc cacgcttcaa agagttctgg atcgagcgtc
     3901 caaaaccgac tgataaacgt ttaaccattt atgcattgct tgactcgccg cgtgcgacag
     3961 gtgcttacaa attcgtggtt atgccagggc gtgacacggt tgtggatgtg cagtcgaaaa
     4021 tctatctgcg cgataaagtc ggcaaactgg gggttgcacc gttaaccagt atgttcctgt
     4081 ttgggccgaa ccaaccgtcg cctgcaaata actatcgtcc ggagttgcac gactctaacg
     4141 gtctgtctat ccatgctggt aatggcgaat ggatctggcg tccgttgaat aacccgaaac
     4201 atttagcggt cagcagcttc tccatggaaa acccgcaagg ctttggtctg ttgcagcgag
     4261 gtcgtgattt ctcccgcttt gaagatctcg atgatcgtta cgatctccgt ccaagcgcat
     4321 gggtgactcc gaaaggggag tggggcaaag gcagcgttga gctggtggaa attccaacca
     4381 acgatgaaac caacgataac atcgtcgctt actggacgcc ggatcagctg ccggagccgg
     4441 gtaaagagat gaactttaaa tacaccatca ccttcagccg tgatgaagac aaacagcatg
     4501 cgccagataa cgcatgggtg caacaaacgc gtcgttcaac gggggatgtg aagcagtcga
     4561 acctgattcg ccagcctgac ggtactatcg cctttgtggt cgattttact ggcgcagaga
     4621 tgaaaaaact gccagaggat accccggtca cagcgcaaac cagcattggt gataatggtg
     4681 agatagttga aagcacggtg cgctataacc cggttaccaa aggctggcgt ctggtgatgc
     4741 gtgtgaaagt gaaagatgcc aagaaaacca ctgaaatgcg tgctgcgctg gtgaatgccg
     4801 atcagacgtt gagtgaaacc tggagctacc agttacctgc caatgaataa gacaactgag
     4861 tacattgacg caatgcccat cgccgcaagc gagaaagcag cattgccgaa gactgatatc
     4921 cgcgccgttc atcaggcgct ggatgccgaa caccgcacct gggcgcggga ggatgattcc
     4981 ccgcaaggct cggtaaaggc gcgtctggaa caagcctggc cagattcact tgctgatgga
     5041 cagttaatta aagacgacga agggcgcgat cagcttaagg cgatgctaga agcaaaacgc
     5101 tcctcgatgt ttcccgaccc gtggcgtacc aacccggtag gccgtttctg ggatcgcctg
     5161 cgtggacgcg atgtcacgcc gcgctatctg gctcgtttga ccaaagaaga gcaggagagc
     5221 gagcaaaagt ggcgtaccgt tggtaccatc cgccgttaca ttctgttgat cctgacgctc
     5281 gcgcaaactg ttgtcgcgac ctggtatatg aagaccattc ttccttatca gggttgggcg
     5341 ctgattaatc ctatggatat ggttggtcag gatttgtggg tttcctttat gcagcttctg
     5401 ccttatatgc tgcaaaccgg tatcctgatc ctttttgcgg tactgttctg ttgggtgtcc
     5461 gccggattct ggacggcgtt gatgggcttc ctgcaactgc ttattggtcg cgataaatac
     5521 agtatatctg cgtcaacagt tggcgatgaa ccattaaacc cggagcatcg cacggcgttg
     5581 atcatgccta tctgtaacga agacgtgaac cgtgtttttg ctggcttgcg tgcaacgtgg
     5641 gaatcagtaa aagccaccgg gaatgccaaa cactttgatg tctacattct gagtgacagt
     5701 tataacccgg atatctgcgt cgcagagcaa aaagcctgga tggagcttat cgctgaagtc
     5761 ggtggcgaag gtcagatttt ctatcgccgc cgccgtcgcc gcgtgaagcg taaaagcggt
     5821 aatatcgatg acttctgccg tcgctggggc agccagtaca gctacatggt ggtgctggat
     5881 gctgactcgg taatgaccgg tgattgttta tgcggcctgg tgcgcttgat ggaagccaac
     5941 ccgaacgccg ggatcattca gtcgtcgccg aaagcgtccg gtatggatac gctgtatgcg
     6001 cgctgtcagc agttcgcgac ccgcgtgtat gggccactgt ttacagccgg tttgcacttc
     6061 tggcaacttg gcgagtcgca ctactgggga cataacgcga ttatccgcgt gaaaccattt
     6121 atcgagcact gcgcactggc tccgctgccg ggcgaaggtt cctttgccgg ttcaatcctg
     6181 tcacatgact tcgtagaagc ggcgttgatg cgccgtgcag gttggggggt ctggattgct
     6241 tacgatctcc cgggttctta tgaagaattg ccgcctaact tgcttgatga gctaaaacgt
     6301 gaccgccgct ggtgccacgg taacctgatg aacttccgtc tgttcctggt taagggtatg
     6361 cacccggttc accgtgcggt gttcctgacg ggcgtgatgt cttatctctc cgctccgctg
     6421 tggtttatgt tcctcgcgct ctctactgca ttgcaggtag tgcatgcgtt gaccgaaccg
     6481 caatacttcc tgcaaccacg gcagttgttc ccggtgtggc cgcagtggcg tcctgagctg
     6541 gcgattgcac tttttgcttc gaccatggtg ctgttgttcc tgccgaagtt attgagcatt
     6601 ttgcttatct ggtgcaaagg aacgaaagaa tacggcggct tctggcgcgt tacattatcg
     6661 ttgctgctgg aagtgctgtt ttccgtgctg ctggctccgg tacgcatgct gttccatacg
     6721 gtcttcgttg tcagcgcgtt ccttggctgg gaagtggtgt ggaattcacc gcagcgtgat
     6781 gatgactcca cttcatgggg cgaagcgttc aaacgccacg gctcacagct gctgttgggg
     6841 ttagtgtggg ctgttgggat ggcgtggctg gatctgcgtt tcctgttctg gctggcaccg
     6901 attgtcttct cgttgatcct gtcaccgttt gtttcggtga tttccagccg tgccaccgtt
     6961 ggtctgcgca ccaaacgctg gaaactgttc ctgatcccgg aagagtattc accgccgcag
     7021 gtgctggttg ataccgatcg gttccttgag atgaatcgcc aacgctccct tgacgatggc
     7081 tttatgcacg cagtgtttaa cccgtcattt aacgctctgg caaccgcaat ggcgacagcg
     7141 cgtcaccgcg ccagtaaggt gctggaaatc gcccgtgacc gccacgttga acaggcgctt
     7201 aacgagacgc cagagaagct gaatcgcgat cgtcgcctgg tgctgctaag cgatccggtg
     7261 acgatggccc gtctgcattt ccgcgtctgg aattcccctg aaagatattc ttcatgggtg
     7321 agttattacg aagggataaa gctcaatcca ctggcattgc gtaaaccgga tgcggcttcg
     7381 caataaaaac gtagttgcct gatgcgctac gcttatcagg cctacatcgt tcctgcaatt
     7441 tattgatttt gcaagatttt gtaggtcgga taag
//
LOCUS       DBKEKI010000174         7396 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.176, whole genome shotgun sequence.
ACCESSION   DBKEKI010000174 DBKEKI010000000
VERSION     DBKEKI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.176"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            24..107
                     /gene="rz1"
                     /locus_tag="ACUQPX_003781"
                     /pseudo
     CDS             24..107
                     /gene="rz1"
                     /locus_tag="ACUQPX_003781"
                     /inference="COORDINATES: protein motif:HMM:NF017860.6"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap.; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
     gene            92..232
                     /locus_tag="ACUQPX_003782"
                     /pseudo
     CDS             92..232
                     /locus_tag="ACUQPX_003782"
                     /inference="COORDINATES: protein motif:HMM:NF015218.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            complement(321..833)
                     /locus_tag="ACUQPX_003783"
     CDS             complement(321..833)
                     /locus_tag="ACUQPX_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HHV9058305.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1028..1792
                     /locus_tag="ACUQPX_003784"
     CDS             1028..1792
                     /locus_tag="ACUQPX_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058306.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGKKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1743..3146
                     /locus_tag="ACUQPX_003785"
     CDS             1743..3146
                     /locus_tag="ACUQPX_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HHV9058307.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3146..4612
                     /locus_tag="ACUQPX_003786"
                     /pseudo
     CDS             3146..4612
                     /locus_tag="ACUQPX_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4503..5246
                     /locus_tag="ACUQPX_003787"
     CDS             4503..5246
                     /locus_tag="ACUQPX_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HHV9058308.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQP
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5250..6470
                     /locus_tag="ACUQPX_003788"
     CDS             5250..6470
                     /locus_tag="ACUQPX_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HHV9058309.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6474..6981
                     /locus_tag="ACUQPX_003789"
                     /pseudo
     CDS             6474..6981
                     /locus_tag="ACUQPX_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="structural cement protein Gp24"
     gene            6993..>7396
                     /locus_tag="ACUQPX_003790"
     CDS             6993..>7396
                     /locus_tag="ACUQPX_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid family protein"
                     /protein_id="HHV9058310.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DISIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNQLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQM"
BASE COUNT         1951 a         1774 c         2034 g         1637 t
ORIGIN      
        1 tgctcttcgg cgcaagcttg ataatggtgg tcgggtgctc gtcaaaggaa aatgccctgt
       61 gccatcctca gccgaaacct ccagcgcctc cggcatgggc aatgatgcca ccgtcgaact
      121 ctctccagtt gctggacgaa acgttctcgg tatccgggac ggaatcatca gcgaccagac
      181 agcattgaga atgcttcagg agtacatcag gatccaatgc cttggggggt agcggtaatt
      241 ttactcatta tccttcaaat cagattctgt tgtcagagga atgggggaag ctggattcgc
      301 aagagctttt tatgggcagt ttagctaaaa aaaggcaaca cgaagctaaa actaccaaca
      361 taaagtgctg accagctagc agctgaaatc agactcactg tatacacttt cactggatga
      421 agttgcaaca ttcctgccac caaaggagca atgtaacgca gcacggcgat aaagcgcgaa
      481 gtgaaaagta ttgacacaga gttattttgc aactgaaggc gaacccgttc tattgtattg
      541 gaacggctcg atattataca tgcaagttga gggatatgcc caattattat accaagatgg
      601 taattaacga tagtaccgca ccatgcgcca ctcatgaccg tgattccagc ttcccatggt
      661 gaaagagtcg tcttgctgac tgtaattaca gtggccatca taattgatgc tggtggcaat
      721 atcgccgaaa tgagtaaagt ggattttgtg aacgctatta taaaaagaag cccccatagg
      781 cgatatggat gaagtacaaa atagttcatc aaagcattta tccactccat caatgtcttt
      841 ccttctgtga atttttcttt gctatttaac cgtgatacga cttaatttag gcttaaacac
      901 cttacattta caaggtactc ctgtgggggg ccctgccacg gagcgtcgca ggagtgagtg
      961 atgaggaaga attaaagcaa cctattgtga caggcagcga taacagtaaa tcacaggaag
     1021 cataatcatg gcaaaaccgg actgggaggc catcgaatcg gcataccggg ccggagtcct
     1081 tagcctccgt gatataggca agaaatacgg cgttacagaa ggggctatca ggaagagggc
     1141 caaaaagctt ggttgggcac gcagtggcgg tacgcaggtt tgcaaaaatg gtacgcaaaa
     1201 aaggaaagtg cgtaccagca gaaagcctgc cattactggc cttacacaaa aaagtacgca
     1261 actaaaaaca gaatctacac cggatacgaa accgatacgc ggaatgcgta ccgatccccc
     1321 gactaaccca ttccaacccg gtaaccagca ggcattaaaa cacggtggtt atgcccgtcg
     1381 ccttctgctc aaagatgagg tgatagagga cgctaaagcg ttgacgctcg aggacgaatt
     1441 atttcgcctt cgtgctaaca accttgtcgc tgcagagaat attggtcggt ggttggtgtc
     1501 gctggaagat gctaatgggg accaggaaag gaagatgctg attgaaaata tcagcgccgc
     1561 cgagaaagca atgatgcgca atacagttcg tattgagtcc atcgttggca cgcttgcgac
     1621 ggtaggaaaa atatttgctg atacagccta ccgcaaggcc gccactgata aggtgtctct
     1681 ggaggctgat cgtcttcgcc gtgatgcagg tattgatgat ggcaatggag agcgtgacct
     1741 caatgacttc tactctgaca tccaaaccga cgctgaatcc ggctttacgt agtttctgga
     1801 ctatgcgggc acgtaacaaa gtgctttatg gtggtcggtc atcgtcaaaa tcatgggatg
     1861 ccgctggcat tgccatattt ctgtcgaata aatacaccct gcgtttttgt tgtgcccgtc
     1921 agatccagaa taaaatcgaa gagtcggtgt ataccctgct caaaattcag atagacaggt
     1981 ttggtctgcg gcaccgtttc cgtattctga acaacaaaat cattaaccgg gttactggct
     2041 cggaatttgt tttttatgga ttatggcgca acatcgaaga aattaagtca ctggagggga
     2101 tcgatgtgtt gtggctggaa gaagcccacg cactgacgga ataccagtgg aaaattctgg
     2161 agccaacgat ccgtaaagag ggttcggaat gctggttcat attcaacccc ggacttgtta
     2221 ctgatttcgt ctggcgcaac ttcgttgttg atccgcccga aggcactctc atccgcaaaa
     2281 ttaactatga cgaaaatccg tttctgtctg acaccatgct taaggttatc gacgcggcgc
     2341 gacgccgtga tccggatggt tttaaacatg tgtatgaggg cgttccggag tctgatgatg
     2401 atgcggcaat catcaaactg tcttggatag aagccgcagt ggatgcgcac aaaacgttaa
     2461 atttcgaacc cagtggaaga aagcgtattg gctttgacgt ggctgacagt ggtacagata
     2521 agtgcgctaa cgtttaccgt cacggatccg ttgttttctg ggccgacgaa tggaaggcca
     2581 aagaagatga attactgaag agctgccagc gtacttatca ggcggcgctg gagcgtgaag
     2641 cagatattgt ttacgactct atcggtgttg gtgcgtctgc cggtgctaaa ttctctgaaa
     2701 ttaacgctga ccggaagagc gagaacgcat acgcgcgacg tgtgaattac cagaggttta
     2761 acgccggtgc tggtgtgcat gagccagatg acgagtacaa cggcatcccc aacaaagact
     2821 ttttcgcaaa tcttaaggct caggcatggt ggctggtggc tgaccgtttc agaaatacgt
     2881 ttaacgccat taacaacgga gaacagtatc ctgtggatga gctgatcagc atagattctc
     2941 gttgtccgtt gcttgaaaag ctgaaactgg aactgacaac acctcatcgt gatttcgacc
     3001 gtaacggacg tgtgatggtc gaaagtaaaa aagacctcgc aaaacgcgag ataccgtcac
     3061 caaacgttgc tgatgcattc attatggcct tcgcgccaat cgatacatcg ctggatatct
     3121 gggaacagct ggggagacag gcctgatggc acgaaacaaa caagccctgc ggcgaactgt
     3181 gcaggccaca gctgatggtt atgagaattt tattgcccgc gtagggatgc agacacctaa
     3241 ccagcactca gcatccacct accgggctaa tttcactagt cgtaaccgca tgctggtgga
     3301 atggtcctat cgttcatcct ggatcatcgg cgaagcagtc gatgctatcc cggatgatat
     3361 gacccgcaaa ggcattcgca tcacttcgga aattgatgca aaagatcgtg gcattctcga
     3421 atcacaactg gatgagttgc aaatctggga tgcgctgaat gacgtgctga aatggtcgcg
     3481 cctctacggc ggcgcggtgg gtttcatcat gattgagggg caggcaccaa tgaccccgct
     3541 gcgacccgaa accatcggta agggcaagtt taaggggatt ctcccgctcg accgctggat
     3601 ggtcgacccg gcactgaccc gccgcattaa agatatgggg ccggacctgg gtaaacctga
     3661 gttttacgat gtggtgacca cagcaacggg aattcctgcc tagcgcattc atcacagtcg
     3721 cctgattcgc tttgatggcg tcacgttgcc atttcagcag aagatgaccg agaacgaatg
     3781 gggaatgtcg gttgtagagc gtatctggga tcgtcttacc gcgttcgaca gcgctactgt
     3841 cggcgcggcg cagctggtct acaaggcgca tttgcgtacc tacagcgtgg agaagctacg
     3901 cgagcttatc gcacttggtg gtcctgcgta tgaagcgttg ctgaagaata tcgacctgat
     3961 tcgacagttc cagagtaatg aaggcatgac gctcatggac tcgcgggata agtttgaaac
     4021 ccatcagtac agcttcagtg gtctggatga catcctttcg cagtttgcag aacagattag
     4081 tggcgctgtt ggtatcccac tggtgcggtt gttcggacag tccccgaaag gattttctac
     4141 cggtgatgca gaccttgcca actattacga tcgcatcagt tcgttgcagg agaggcgttt
     4201 acgtcttccg gtgcggcgga tactggacat catgcatcgt tcggaacttg gcaagccgct
     4261 gccggacgat ttcacgtttg agtttaaccc gctctggcaa atgtctgatg tcgatcgctc
     4321 aacggtggcg ttaaacacca ccaacgcaat cagtacggcg ctgggtgatg gtctgatgac
     4381 actgaaagcc gctatgactg atttgcgaga aaattctgac gtaaccggca tcggggcatc
     4441 cattaccgac gaggacatcg agaatgccga agatgaagcg ccgcccggca tcggcgaacc
     4501 tgatgacgaa ccgcaggagc cgtcaggcgg aaatccggta tcgaaccagc ctacgcagga
     4561 tagcgagggc ggtcggagac atcgtaaatg gtcgctacga tggttcaaat gacagtatca
     4621 cggaaattat tgcggcgctg gagcgttaca gtgaaatcat caccccctgg gcgacaaagg
     4681 tcgcggaaaa ctttactgcg gacctaaccc ggcagaacga gaaagtttgg cggcaacaca
     4741 gcaagaacat cagtcgcgag ctccgcaatc ttgtggaaag cgctcctgtg ggccaggtga
     4801 tgcaacccat catcgccgaa caggtcaagt acatcaaatc tctgcctctc gaggccgcag
     4861 acagggtgta cgacatccag aacaaagcga tagaggctgt tgtcaccggt ggtcgggcgg
     4921 agcagtttgc taaagagatt gcatctaccg gcgatgttgc taagtctagg gccgatctga
     4981 ttgcccgaac ggaactggga agagcaacgg gcgcgctgga tatggcccga gcgatagcta
     5041 ttggctcgga tggttatatt tggcgtacag ccgatgatgg cgatgtccga gattcccacg
     5101 atcacatgaa aggtaaattc gtccgctggg attcacctcc aactttggac ggcatgaccg
     5161 gccacgcggg cgagctgcca aactgccgct gctataaaga gatcgtgttt gttcgcgttc
     5221 cattcgcaat gaaaagggca gcataaccca tgaaatactt ttttgagacc aggctcgggg
     5281 aaacccgata ccgcctggct gacggctcgt tgctgtgcaa agacgtgccg ataggacgaa
     5341 caggtaagca gctctatggt gctgatgacc tgccaaaact gaaacccgat aagttcggtg
     5401 aaatagtcgt cacgcgttct cctgagcagg tattccatcc ggccacgctt gcctcattcg
     5461 aagggatgag catcacgatc ctgcatcctg aagatgaaaa cgggaatgtg cggctggtaa
     5521 atcccgagaa ctggaaagag cttgctgtcg ggcacctcca gaatgtccgg cgcgggacgg
     5581 gtgagcagtc tgatttgatg ctggctgacc ttatcgtcaa agacgaaagc gccattcagc
     5641 ttatcgaaga tggcctgcgc gaagtgtcgt gcggctatga cgcggagtac gagcagaccg
     5701 agccaggtaa agccgagcag gtcgatatta ccggaaacca tgtggctctt gtccctaaag
     5761 gcagagccgg aaatcgttgt gcaattggag acagagacac aatggcaaat caaaagaaaa
     5821 actggtggaa ccgcatgcgt gcggccatca agacaggaga tgccgacacc atgaacgaac
     5881 tggtggagtc ggctcccgca tcggttacag gagatgaggg ggatttgccg cagggcgtta
     5941 atctcaacat caacctgtcc ccgcagcaac cactaccgga caaagcacca gagatgggcg
     6001 gagatccaac cggcgacagt gatgatgacc tcaaaacatt actgaaagcc ctgctggcta
     6061 agctggaaag aaatgccacg ggcgataacg ataataagcc tgacgataat ccgaccggtg
     6121 acggcgagga cgatgaagag gaaaccacga ttactggtga ctcagcctgg cgtgccgaag
     6181 ttatcgttcc gggtatcgat ctgagccgta agatgaaacc gaccgcgttc aaacgcgagg
     6241 ttctggcttc tgctgacaaa acgctggttc gccagatagt cggtgatgcg gatatccgca
     6301 aattaccgaa acaatcggtc gacatggcgt ttaatgccgt gtctgaggtt gccaaagggc
     6361 gaaacacccg cgccaccacc ggcgatgcac agcgcctaaa catgggcatg accagtatcg
     6421 cttccctgaa caaacaaaac gctgaattct gggcaaaccg taaagggtaa aaaatgaata
     6481 atgtatttct gtaccggatg cctgttggca ttgccggggc tgtctctcgc ccgcaggact
     6541 taaccgtcga accggtggtc cttaaatccg ataacgcctt cgctgcctat ggcctggctg
     6601 gtaaatacga tgatgacggt tttttcgtgc cgctggcaga tggtgatacc gcagacaagg
     6661 tgaaggggat ctacgtgcgc ccttatccga ccacgtcgca gccggacatg gttcgccagg
     6721 tgggaacagg caagaacttc ccgggcgacg ccatgaagcg tggctacgtg accgttaatc
     6781 tcggttctga ttttgatgcc agcactatca aaaaaggcga tccggtatac gttgtcgtca
     6841 tccactgatg aatccatcaa agtgccgctg ggtggattca tgtccacgtc agtcagtggc
     6901 aaaaacgtgg tgctgaccaa cgctgaattc acaggtgccg gtgatgctga cggcaatgca
     6961 gaaatttcct ggaagattta aggaacagac gaatgattac ttttgatcag gcaaccgttg
     7021 acagctctgg tgcctttctc atcggggagc tggagcgact cgaccagacg ctgaacctgc
     7081 cactggtggg gtacacctgg agccgcgata ttcaactgcg tgaagacatc tccatcgcag
     7141 atgacatttc cagctggacg aataccagct tcgccgctgc gggtactggc gcaaatccga
     7201 atggaaaaaa ctgggtaggc aaagactcaa ccgctattgc tggcgtgaac gtggatatcg
     7261 gcaaatccgg taaccagctg aacctgtggg ggatggaact tggctggacg gtcatagaat
     7321 tgcaggctgc tcagcaggtc ggccgcccga ttgatacgca gaagtatgac ggtatgcaac
     7381 tgaaatggca gatgga
//
LOCUS       DBKEKI010000175         7383 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.177, whole genome shotgun sequence.
ACCESSION   DBKEKI010000175 DBKEKI010000000
VERSION     DBKEKI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.177"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            46..150
                     /locus_tag="ACUQPX_003791"
     CDS             46..150
                     /locus_tag="ACUQPX_003791"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HHV9058311.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(199..1452)
                     /locus_tag="ACUQPX_003792"
     CDS             complement(199..1452)
                     /locus_tag="ACUQPX_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9058312.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTHYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1559..2452
                     /locus_tag="ACUQPX_003793"
     CDS             1559..2452
                     /locus_tag="ACUQPX_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHV9058313.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2587..3807
                     /gene="mlc"
                     /locus_tag="ACUQPX_003794"
                     /gene_synonym="dgsA"
     CDS             2587..3807
                     /gene="mlc"
                     /locus_tag="ACUQPX_003794"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HHV9058314.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3932..4627
                     /gene="bioD"
                     /locus_tag="ACUQPX_003795"
     CDS             3932..4627
                     /gene="bioD"
                     /locus_tag="ACUQPX_003795"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HHV9058315.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4580..5872)
                     /gene="clcB"
                     /locus_tag="ACUQPX_003796"
     CDS             complement(4580..5872)
                     /gene="clcB"
                     /locus_tag="ACUQPX_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HHV9058316.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6031..6645)
                     /gene="dmsD"
                     /locus_tag="ACUQPX_003797"
     CDS             complement(6031..6645)
                     /gene="dmsD"
                     /locus_tag="ACUQPX_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HHV9058317.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6688..>7383)
                     /gene="ynfH"
                     /locus_tag="ACUQPX_003798"
     CDS             complement(6688..>7383)
                     /gene="ynfH"
                     /locus_tag="ACUQPX_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HHV9058318.1"
                     /translation="MGIGFIASVMHLGSPLRAFNSLNLIGASGLSNEIAAGSIFFAVG
                     GLWWLVAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFL
                     TVLLSGPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQA
                     SALVPDYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLF
                     YGLHMTVGMAIAG"
BASE COUNT         1971 a         1873 c         1934 g         1605 t
ORIGIN      
        1 agcggtagaa taactgcgca tcagtaataa atgacacaca gcaaaatgaa tccgtttatt
       61 tgggtactta taatcctgat gacgctagat gcgctgcggg aattggctgg cgcttcgtct
      121 attttaggat ggctattaac gttggtttga gcaggcaata agtccggacg ggtatttacc
      181 gcagtccgga cttatttttc aggcgtgcag acgacgatgc aaacgcgtcc cgaccagcag
      241 agcaatgacc agcatcagag caataaatgc gccgacgccg ttccagccat agttatgcca
      301 gaaaacacca cccagcgtcc cggcaatact cgaccccaga tagtaactga acagatacag
      361 cgaggaggcc tggcctttag cgcgttttgc gcgggggccg atccagctgc tggctactga
      421 gtgggctgcg aagaatccgg ctgagaagag taacattccg gcaaagatca gccacagcga
      481 gctaaataag gtcatcagta aaccaaacag cataactccc gtcgaaaaca acatcaccgg
      541 accgcgccca tagtgggtgg tcatggttcc ggctttgggt gagctccatg taccggtcaa
      601 ataagccagc gataataagc caaccacggc ctgactgaca tgccaggggg aaagcatcaa
      661 ccgatagccg atgtaattaa acagcgtgac gaacgacccc atcagcaaaa agccttctgc
      721 gaacaataac ggtaatcccc ggtcacgcca gtgcagacga aagttgataa ataacgtctt
      781 agggcgcagc gaagtcgggc aaaaatggcg tgattcaggg aggattttcc agaacatcaa
      841 cgccgaggcc agcgcgaaac aaccgattgc cgccagagca attcgccagt tgaaaaagtc
      901 cgtgaagaca ccactaatta agcgtccgct catgccgcca attgagttgc cgctgatata
      961 caaccccatc gaaaaggcca cgaaactggg gtggatttcc tcgctaagat aagtcatgcc
     1021 aacagctgcc acgccactta acgaaagccc aatcaaggcg cgcataatca aaatgccgtg
     1081 ccagctggtc atcattgtcg aaagtaacgt acaaatggag gccaacagta gcgccgtgac
     1141 catcactggt ttgcgaccaa tggcatcgga tagcgggcca gtaaatagca aaccgatagc
     1201 caacatcgcc gtggaaatgg acagtgaaat actactgttc gcgggcgtta agccaaactc
     1261 ctgcgaaagc accggaagga taggctgcac acaatagaga agtgcaaagg ttgccagtcc
     1321 ggcagagaac agcgccaggg tgacgcgcat aaattgcggc gtaccgcgtt taataaattg
     1381 atttggctga gaaatgcttt gcttgtcagt gtcgcttgcc ggagcgccat caacagttgt
     1441 agtacggctc acttgaaatc cttgctaaat atgcctgtgg atcaggctta tacatagggt
     1501 aggaaaatcg aattgttctg tctaatatat taataatctc aaataagatg ttttaaatat
     1561 gaatattgaa cttcgtcatc tgcgttactt tgttgctgtt gcggaagagc tgcattttgg
     1621 gcgcgcggct gcccgcctga atatttcgca accgccgcta agtcagcaga ttcaggcact
     1681 ggagcaacaa atcggtgccc gactgctggc acgaactaat cgcagtgtat tgctgacggc
     1741 agcaggaaaa cagtttcttg cagatagtcg gcaaatcctg tctatggtgg atgacgctgc
     1801 cgctcgcgct gaaagactgc atcagggtga agcgggggag ttgcgtattg gttttacttc
     1861 gtccgctcct tttattcggg cggtgtccga tacgttatcg ctgtttcgcc gtgattatcc
     1921 tgatgtccat ttacaaaccc gcgaaatgaa cactcgcgag caaatcgctc cgctcattga
     1981 aggaacgctg gatatgggat tgctgcgtaa cacagcgtta ccggagacgc ttgaacacgc
     2041 agtcatcgtc catgaaccgc ttatggcgat gatcccgcac gatcatcccc tggcaaataa
     2101 tccgagtgta acactggctg aactggcgaa agaacccttt gtcttttttg atccgcacgt
     2161 cgggactggg ctgtatgacg atattctcgg actgatgcga cgttaccatt tgacgcccgt
     2221 catcactcag gaggtgggcg aggcaatgac catcatcggt ctggtttccg ccggtctggg
     2281 tgtttcaatt ttgcctgcgt catttaaacg tgttcagctc aacgaaatgc gctgggtgcc
     2341 gattgctgaa gaggatgcgg tttctgaaat gtggttggtc tggccgaaac atcatgaaca
     2401 aagtccagct gcgcgtaact ttcgtattca tctgctgaat gctctcaggt gagggaaatt
     2461 tcagcgaaaa agcccgaaaa atgtgctgtt aatcacatgc ctaagtaaaa atttgacgac
     2521 acgtattgaa gtgcttcacc atagcctaca gattatttcg gagcgcgaaa atatagggag
     2581 tatgcggtgg ttgctgaaaa ccagcctggg cacattgatc aaataaagca gaccaacgcg
     2641 ggcgcggttt atcgcctgat tgatcagctt ggtccagtct cgcgtatcga tctttcccgt
     2701 ctggcgcagc tggctcctgc cagtatcact aaaattgtcc gtgagatgct cgaagcacac
     2761 ctggtgcgag agctggaaat caaagaagcg gggaaccgtg gccgtccggc ggtggggctg
     2821 gtggttgaaa ctgaagcctg gcactatctt tctctgcgca ttagtcgcgg ggagattttc
     2881 cttgctctgc gcgatctgag cagcaaactg gtggtggaag agtcgcagga actggcgtta
     2941 aaagatgact tgccattgct ggatcgtatc atttcccata tcgatcagtt ttttatccgc
     3001 caccagaaaa aacttgagcg tctaacttcg attgccataa ccctgccggg aattattgat
     3061 acggaaaatg gtattgtaca tcgcatgccg ttctacgagg atgtaaaaga gatgccgctc
     3121 ggcgaggcgc tggagcagca taccggcgta ccggtttata ttcagcatga tatcagcgca
     3181 tggacgatgg cagaggcctt gtttggtgcc tcacgcgggg cgcgcgatgt gattcaggtg
     3241 gttatcgatc acaacgtggg ggtgggcgtc attaccgatg gtcatctgct acacgcaggc
     3301 agcagtagtc tcgtggaaat aggccacaca caggtcgacc catatggtaa acgctgttat
     3361 tgcgggaacc acggctgcct cgaaaccatc gccagtgtgg acagtattct tgagctggca
     3421 cagctgcgtc tcaatcaatc catgagctcg atgttacatg ggcaaccgtt aaccgtggac
     3481 tcattgtgtc aggcggcatt gcgcggcgat ctactggcaa aagacatcat tatcggggtg
     3541 ggcgcgcatg tagggcgcat tcttgccatc atggtgaatt tatttaaccc acaaaaaata
     3601 ctgattggct caccgttaag taaagcggca gatatcctct tcccggtcat ctcagacagc
     3661 atccgtcagc aggcccttcc tgcgtatagt cagcacatta gcgttgagag tactcagttt
     3721 tctaaccagg gcacgatggc aggcgctgca ctggtaaaag acgcgatgta taacggttct
     3781 ttgttgattc gtctgttgca gggttaacat tttttaactg ttctaccaaa atttgcgcta
     3841 tctcaatttg ggccaggaaa gcataactta gactttcaag gttaattatt ttcctagttt
     3901 atatttgtga agcataacgg tggagttagt gatgctgaag cgtttcttta ttaccggtac
     3961 agacacttct gtagggaaaa cggtggtttc ccgcgcattg ctacaagcgt tagcctccca
     4021 gggaaaaacg gttgcgggat ataaacccgt agcgaagggg agcaaagaga cacccgaagg
     4081 gctgcgtaat aaagatgcct tggtgttgca gagtgtttca accatcgaac tgccttatga
     4141 agcagttaat cctatcgcgt taagcgaaga agaaagtagc gtggcgcaca gttgcccaat
     4201 caattacacc ctcatttcaa acggcctggc aaacctgacc gataaagtcg atcatgtcgt
     4261 ggtagaaggg actggcggct ggcgcagtct gatgaatgat ttgcgtccac tctctgaatg
     4321 ggtagtgcag gaacaactgc cggtgttgat ggttgtcggt attcaggaag gttgcattaa
     4381 ccatgcactg ctaacagctc aggcgatcgc caacgacggg ctgccgctca ttggctgggt
     4441 ggctaaccga atcaacccag gactggcgca ttatgcggaa atcattgatg tgctgggtaa
     4501 aaaacttccg gcaccgctca ttggtgaact gccttatctg ccgcgcgccg aacagcgtga
     4561 actggggcaa tacatccgct tagctatgct gcgcagtgtg ctggcggtag atagagtcac
     4621 ggtgtaacgt ccgcgaaatt accgacgcaa ttacgcaggc aatcaataaa ccggggagta
     4681 gctgatactc cccggtcatt tcacatatca tcaacgtcga cataatcggc gcgtgcgtgg
     4741 ttgccgccaa cagtgtcgcc atcccggtca atccgagtaa aagcgtaatt tcttcgccat
     4801 cagggaacca taatcccagg ctacgaccat acaacatgcc aatggcaaga ccgataaata
     4861 gcgtcggtgt aaagacacca ccgggtgcgc cggaaccgct actcgccagc acggcacaca
     4921 gtttacaaag gaagatcccg gcaatgatca ttaacagtgg tggggcggtt aaaaaggatt
     4981 gtacggtgct atagccgttg ccccacactg caggtgtgaa cagggaaagc agacccacga
     5041 tcaacccgcc tagtgccagt tgccagggcg gcgcaagttt gagactcaca aatccacgat
     5101 gacaggcgtt cattaacgtt aacaacagtg gtccgcacag acctgccagc acacctgtac
     5161 tgataatcag cgcatagtca cgagcctgaa ccgtcactga gagttgtacg ttgtagagta
     5221 acgcgtcgct atgattaatc agattgctaa ccagcaatgc cacgacggcg gaaataatca
     5281 ccgggccgag agaggccaac atcatagtgc caaacagcac ttcggctata aataaactgc
     5341 cagcaagcgg ggcacgatag gccgcagcca ttcccgccgc ggccccacag gcgatccata
     5401 atttccattc ctggcgtggc gtaaaacgtt gggcaaaaca ggaggcggca agggcagcta
     5461 aaagaatcat cgcaccttcg cgaccaattg cactgccgct ggttactacc agcagcgagg
     5521 caagcgattt aaccaggctt gctgcgtaat cgaactgtcc atcggtttgc aacgcttcca
     5581 tgtaatcggt cggcgcatga gggcgttgtt gggtaaattt ctgccagccc atcagcaaca
     5641 aacccgccgc cagtccgccg agcgccggag ttagcagccg tcgccagggg gaaaggtttg
     5701 tcgctgcatt gaccagactg ccggagtcat tattgaggaa caaccactcc agtagcagca
     5761 tcgcatgacg aaacccggca acggcaaaga ccgcgagaat accgacgact gttgcgataa
     5821 gcagacggcg gaacatagtg cgcagatccg gatagatgtg tagatgatgc atgagcagaa
     5881 atgggcaatg aaacacgggc gactattttg cggcaaaaga ggccaaccgc aaaagcagtg
     5941 cgtgctttaa gagataaaaa aacgtgacat tgtaacgtat tattgccgaa tcgcgcgtaa
     6001 tcgtcaccat ccggcaatat tacggtgatc ctatcgaaag agtggtttaa ccgcgacagg
     6061 aatcaacagt tgtgattgcc attgcgccag cgttaagcga gccagttcac ccagtgcacg
     6121 gtagaaaggg tgttctgctt tttcgataaa aacatcaaga aaacgtgttg accacggaaa
     6181 aaggtgccat gccagcaatt cttcgcattc tgtctggcga ccattctccg ccaaccacgc
     6241 agccatcaac agcagtgaac caaaatgatc ttccggttcg ttttgcttca tttcaaactg
     6301 aatgcctttc tcgcgcatcc actgacgaag tgccaatgtt gaatcgccaa acagcacaga
     6361 ttcgcgatcc agccagaccg acccccacgg cggagacggc agtgcccacg ggccgacaaa
     6421 caaacgctgc caggcctggg cgtgagtctc ttcacactgt gtctgaaaag ccgtcaccag
     6481 cggcgctaat gacgcttctg gtagaggcca ctgagtttcc cagccgtcac tggtgagtac
     6541 cgcaaccaga ggtgcggctt ccgcactctc tggagcgtaa taaaacagcg cgcccaacac
     6601 gcgcgccgcg acagaaaaat tatcttgctg tgaaaaatgg gtcattccta acatcctgaa
     6661 atgtgcgggt tgccccgcac caatttgtta acctgcaata gccattccta ctgtcatgtg
     6721 caggccataa aagagcacac gcccgatcat ctcaccaaca agtatgagga ttagccccag
     6781 aacaagtccg gcaacgtgag gttcacggcg acgaatgagc ggacaaagcc agcatccaag
     6841 acctgcgcat aacaatacca cacgccagac ctgtaaggag gcgtaatctg gtaccagtgc
     6901 gctggcttgt tgcacggatg agtgaataga cgccagagac aaaccttgca gcacaatcac
     6961 ctcggcacaa gcgatcagcg ccagaacgct aatgatggca aatggtgtgg tattaaatgt
     7021 aacgcgggct gcccgcagaa ttgcggcagc aagtattgga ccgctcaaca ataccgtcag
     7081 gaagaaagcc agcgtagtgt aaccgttatg ccaggttggc acggtgtcga tttgatacac
     7141 gcaggtcatc atccagacga aaatgacgcc gagcgccatg ctgacaagta accagagttt
     7201 ccccaacgct tgcggcattt taccgatgac cgccaccagc caccatagtc cgccaacggc
     7261 aaaaaatatg gaaccggcag caatctcgtt actcagacca gaagctccga ttagattcag
     7321 cgagttaaac gctcgcagtg gcgatcccag gtgcatgaca gaggcaataa acccgatgcc
     7381 cat
//
LOCUS       DBKEKI010000176         7374 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.178, whole genome shotgun sequence.
ACCESSION   DBKEKI010000176 DBKEKI010000000
VERSION     DBKEKI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.178"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..866)
                     /gene="glxR"
                     /locus_tag="ACUQPX_003799"
     CDS             complement(<1..866)
                     /gene="glxR"
                     /locus_tag="ACUQPX_003799"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HHV9058319.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMAN"
     gene            complement(966..1742)
                     /gene="hyi"
                     /locus_tag="ACUQPX_003800"
     CDS             complement(966..1742)
                     /gene="hyi"
                     /locus_tag="ACUQPX_003800"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HHV9058320.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDILGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWIGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(1755..3536)
                     /gene="gcl"
                     /locus_tag="ACUQPX_003801"
     CDS             complement(1755..3536)
                     /gene="gcl"
                     /locus_tag="ACUQPX_003801"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HHV9058321.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGDIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QALRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3626..4441)
                     /gene="allR"
                     /locus_tag="ACUQPX_003802"
     CDS             complement(3626..4441)
                     /gene="allR"
                     /locus_tag="ACUQPX_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HHV9058322.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4519..5001)
                     /gene="allA"
                     /locus_tag="ACUQPX_003803"
                     /pseudo
     CDS             complement(4519..5001)
                     /gene="allA"
                     /locus_tag="ACUQPX_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
     gene            5231..6157
                     /gene="allS"
                     /locus_tag="ACUQPX_003804"
     CDS             5231..6157
                     /gene="allS"
                     /locus_tag="ACUQPX_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HHV9058323.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6226..7320
                     /gene="mnmH"
                     /locus_tag="ACUQPX_003805"
     CDS             6226..7320
                     /gene="mnmH"
                     /locus_tag="ACUQPX_003805"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HHV9058324.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
BASE COUNT         1880 a         1955 c         1785 g         1754 t
ORIGIN      
        1 tggttagcca ttaattccag cgcctgcact aacgcagagt gatccaactg gctgccaccg
       61 tttgccgcac aggtattaaa taactcctgg caggtcgcag tgtttggcag gttcagcgca
      121 agtgctttcg cactttgcag tgccaggttg agatctttct ggtgcagagc gattttgaag
      181 cccggattaa aggtgcgttt aatcatgcgc tcaccgtgaa cttccagaat acgtgaggaa
      241 gcaaagccgc ccatcagcgc ctggcgcaca cgtaccgggt ccgcaccggc ttttgaagca
      301 aatagcaggg cttcagaaac cgcttcaata ttgagcgcca caataatctg atttgccact
      361 ttgcaggttt gaccatcgcc gttaccgccc acgagggtga tatttttacc gagcagttca
      421 aacagcggtt taacacgttc aaataccgct tcatcaccgc caaccataat cgacaacgtc
      481 ccttcacgcg caccgatttc accgccggag actggcgcat cgagataatc gccgcccagt
      541 tcattcacct gacgagcgaa acgcttagtt tcaatcgggg aaatggagct catatcaaca
      601 atggttttgc ccttcagcga ggctttggta caaccatttt caccgaacag aacttcttca
      661 acctgaggtg tgtccggcac cataataaaa atgatgtccg atgcttccgt tacctggcga
      721 gcagtttcaa cactgacggc acccagtgac agtaattcat cagcaaccgg tccaatggtc
      781 gtgacatgta attgatgacc ggcacgcgcc agattaatgg ccatcggtgt acccataatg
      841 cctaagccaa taaatcccag tttcataatt aacctctttt aaatttcgct tttcctgaat
      901 tcagacaaca cgatcccctg ccaacttagc ggcctaaaaa agcattgtct gaatagcgtt
      961 acgttttaac ggtacggatc catccagcgt aaaccagctt ccgtggtggt ttggggttta
     1021 tattcacacc caatccaacc gttgtagtca gagttttcaa ttaccgtaaa gagatattcg
     1081 taattaattt ctccggttcc cggttcgccg cgatgcggat tatcagcaat ttgcaggtga
     1141 ccaattttat cggcccactg agtcatggtg ttggttaatt caccttccat ccgctgcatg
     1201 tgataaatgt catactgaat tttcaaattg cagcaaccaa catcatcaat caatttcagc
     1261 gcctgccgag ttccggtgag atggaaacca agaatatcaa aatggttaat aggttcaatc
     1321 agtaataaaa tatcttcttt catcagcata ttcgcggcat aacgcaggtt ttctacaagc
     1381 gttgcgtgaa tctgttcact gctgaaacca gccggcgttt taccgaccag acagttaatt
     1441 tttttattac ccagcgcacg ggcataacga atcgctgctg ctacgccatc ccgaaactct
     1501 tcttcacggc caggaataca ggcaataccg cgctcgcccg ccgcccagtc acctgccggt
     1561 aaattgtgca gcgtatgttc gagtttatta ctcgccagta cctgttttaa ttcttcaatg
     1621 tcgtagtcat aaggaaacat aaattcaacg ccgcgaaaac cacactgcgc agctttctca
     1681 aaacgggcga gaaaatcata ttcaccaaat aacatcgata aattagcaga gaaacgtaac
     1741 atcatttatc tcccttattc atagtgcatg aagcaggttt cagtcggtgc atccgctgcg
     1801 ttatcggcga tatcttcaaa ttccatgacg ttatccagtt cgctgcccat cgaaatattg
     1861 gtcacacgct cgagaataac ttccacgact accggtaccc gatattgcgc cattaaggct
     1921 ttcgcctgtt caaaggctgg cgcaatatct tccggtttga agacccgaat agctttacaa
     1981 cctaaacctt ccgctacttt tacgtggtca acaccgtagc cattcacttc actggagttg
     2041 atattctcga aagcgagttg cacgcagtag tccatgtcaa aagcgcgttg tgactgacga
     2101 atcaggccga gataggcgtt attcaccagc acatggatgt acggaatgtt gaactgcgcg
     2161 ccaacggcta actcttcaat caggaactgg aagtcaaagt cgccagaaat cgccaccaca
     2221 ttgcgtttcg gatcagcggc acaaaccccc agcgccgccg gaatcgtcca gcctaacgga
     2281 ccagcctgac cacagttgat ccagtggcgg tctttaaaga catgcagcat ttgtgccgca
     2341 gcgatttgtg acagaccaat agtggtgaca taacaaacat cgcgaccaaa ggctttgttc
     2401 atctcttcat acacgcgctg cggtttcacc ggcacgttgt cgaagtgggt tttgcgcagc
     2461 aaagtgcgtt tacgctgctg gcagtcggcg acccattctt tacgacacgg cagacgaccc
     2521 gctttttgca tctcctgcgc cacttcaacc agcagtgtca gcgccgcttt agcatcagag
     2581 acaatgccga gatccggaca cagcacgcga ccaatttgcg tcggctcaat atcaatatga
     2641 acgattttgc gcccttcggt gtatttctct accgaaccgg tatgacggtt agcaaaacgg
     2701 ttaccgatac caaacaccat gtcggacgcc agcaacgttg cgttaccgta acgatgcgcg
     2761 gtttgcagac cgaccatccc ggccatcagt tcatgatcgt ccgggataca gccccagccc
     2821 atcagcgttg ggatcaccgg aacgctggtc agttcagcaa actgttgtaa cagtgcagct
     2881 gcgtcagcat tgattacccc gcccccggca acaatcaccg gacgttcggc ctggattaac
     2941 atttctacag ctttttcgat ctgcatacgg ctggcagcag gtttgtagac cggcagcggt
     3001 tcgtacatgt caggatcaaa ctcgatttcc gcaacctgaa cgtcgaacgg taaatccacc
     3061 agtaccggac ccggacgacc agaacgcatc aggtgaaatg cctgttgcag cacgcgaggc
     3121 accagcgccg cttcacgaac tgtaaccgcc attttgctga ccggtttagc aattgcttca
     3181 atatctacgg cctgaaaatc ttctttatgc agacgggcgc gcagtgcctg gccggtaatg
     3241 cacagaatag gaatggaatc agcagaagcg gaatagagcg cggtgatcat gtccgtgccc
     3301 gcaggaccgg aagtccccag acatacgccg atatcccctg ccgttgcgcg ggtataacct
     3361 tccgccatgt gcgaaacacc ttccacatga cgcgccagaa tgtgacgaat accgccgtgc
     3421 ttacgcatcg ctgagtagaa cggattaatt gcagctcccg gaacgccgaa ggcggtggtg
     3481 ataccttctt tctccagcac atacattgcc gcgtcaacgg ctctcatttt tgccatttta
     3541 ttcctacctc tattaattgg aaaaattttc caactttcaa attttgtcaa aaaaacccgc
     3601 tccgcagagc gggatgcgac agacactatg gatgtgcttt cagtcccaac gccgtgctga
     3661 tatcgcgggc ggtgtctctg accagctcac cctgactgac aaaacgatct tctgtcagtc
     3721 ttgatgaagg cccggagatg gagatagcgg caacaacgct accgacatca tcgtaaattg
     3781 ctgaagctat gcaattcaga cctacaacat gctcttcttt atctacggta tagcccagtt
     3841 cacgcgcttg ttccaggtcc ttcagcaagg tgggcatatc cacaagcgta gttggcgtaa
     3901 actgctgcaa accggtttgc agaatgatgc tcatcaactc ctcttccgcc agcggataaa
     3961 gcagcgcttt gcccgcaccg gaagcatgca gcggcagacg actgcccagt ggcgcacaca
     4021 tcctgaccat cgatttacac tctaactgac caattaatac cgcttcattg ccgttacgga
     4081 tcgcgacatt gaccgtttcg ccggaaagta acatcaggcg gcgcataaac ggcccggcga
     4141 cggagaggac atcgcggtta tggatgtacg ccgcaccgac gttaaagaca cctaatccta
     4201 tatgccacca gcctaattga ctgtcctgat agacaaaatc cgctgcttgt aaaaccttca
     4261 gcaagcgaaa ggtcgtggag agcggcaaat ccagattgag agaaatatcg ctaaccgacg
     4321 aacttccccc acttttttcc agatattgca gaatcgcaat tccccgctct aacgcctgtg
     4381 cgcccttctg tgccacaggc tccgcctgtc ctggcctgcc gcgccgtcta acttccgtca
     4441 tacctacgtt aactccgacc tacaagtgag cataaacaat gaacaattag tttatcttat
     4501 gcaagtcggt tgcaggcgtc acgcaaaact gagttcctgt tcaggaatac tttcaacatc
     4561 acagttgtca ctgccgccgc gatcgatggt cagaaaatcg gtgacgcgct gccaggcgaa
     4621 aagtgggtga tgctagacgt tacgatggta attcaccccc tgttcaccgt tggtgataaa
     4681 cgcccgcagc gttgacaggt ctggtttgtc gtcgcctaac gccacgacca ccacaaacac
     4741 ttcacctttc attgggataa aggcctgagt gcccagcgga tgacgttcga gttcgtgaat
     4801 ggtcagcggc agattcgccg gttgcgcgcg gttaatgctg ataagcgtac ggtcttgctc
     4861 aagaatctca accagcgcca aatcgtggta acgctccacc aggccattgt taatatggaa
     4921 aaaatcccgt tgctgcgttt cgattacgtc gccataagca ctaaaggctt cctgacttaa
     4981 cggtaatacc tgaagtttca tctttttgtc cttgtaacat aattccgttc aaccaccgcc
     5041 gaccggtcag cttggtttca ataacaggca atctaaaacc ttaagagaac aacatcaata
     5101 caaaagttgg aatgtttttc caaaactaga tttagtccac aaattgaaca gggctatcat
     5161 gtaaaaatac tgataaatca gaggataaga aaatcacgca acttgcggat ttaaaagggg
     5221 atacctgaag atgttcgatc cagaaacctt gcggactttc attgcggttg ctgaaacagg
     5281 aagtttttca aaagcggcag aacgattatg taaaaccacg gcgacgatca gttatcgcat
     5341 taaacttctg gaagagaata ccggagtagc gctgtttttc cgtacgactc gtagcgtaac
     5401 gttgacagcg gctggcgagc atctactttc ccaggccaga gactggctga gctggctgga
     5461 aagtatgccc agcgagctgc aacaggtgaa tgatggcgtg gaacgccagg tgaatattgt
     5521 catcaacaac ctgctctata acccccaggc cgtcgcccag ttgctggcgt ggctgaatga
     5581 gcgttacccc tttacccagt ttcacatctc ccgacaaatc tatatgggcg tctgggactc
     5641 gctattgtac gaaggttttt cgctggctat cggggtcacg ggaactgagg cgctggcaaa
     5701 tacctttagt cttgatccct taggatcggt gcaatggcga tttgtcatgg cggcggatca
     5761 tccgctggcg aacgttgaag agccgctaac agaagcgcag ttgcgtcgct ttccggcggt
     5821 caatattgaa gacagcgccc gcaccttaac caaacgcgtc gcctggcgat tgccggggca
     5881 aaaagagatt attgttcccg atatggaaac gaaaatcgcc gcccatctgg cgggtgttgg
     5941 cattggtttt ttgccaaaat cactttgcca gtcaatgatc gataatcaac aactggtcag
     6001 ccgggtaatc ccaacgatgc gccctccttc gccattgagt ctggcatggc gcaaatttgg
     6061 cagcggcaaa gcggtagaag atattgtgac cttgtttacc caacgcaggc cggaaatcag
     6121 cggattttta gaaattttcg gcaacccacg cagttaaaaa tcaagtttcc ttgcgcgttg
     6181 gcgcgtaaaa tagcgcgcaa gctgatttga ggttcacttt taactatgca agagagacac
     6241 acggaacagg actatcgtgc cctgctgatt gctgatacgc ccattattga tgttcgcgcc
     6301 cctatcgagt ttgagcaagg cgcaatgccc gccgctatca atctgccgtt aatgaataac
     6361 gatgaacgcg ccgccgttgg cacctgctat aaacagcaag gctcagacgc agcgctggcg
     6421 ctggggcata aactggtggc gggtgaaatt cgtcagcagc gtatgtacgc ctggcgggca
     6481 gcgtgcctgc aaaatccgca aggtattctc tgctgcgccc gtggcggtca acgttcacat
     6541 attgtgcaac gctggctgca tgaagcgggg attgattatc cgctggtgga aggcggttat
     6601 aaggcactgc gccagaccgc gattcaggcg accattgaac tggcacaaaa accgatagtg
     6661 ctgattggcg gttgtaccgg cagcggtaaa acgctattag tgcaacaaca gccaaacggt
     6721 gttgatctgg aagggctggc gcgtcatcgc ggttcggcgt ttggtcgcac gttacaacca
     6781 caacttagcc aggcgagttt tgaaaacctg ctggctgccg aaatgctaaa aaccgacgcc
     6841 cgtcaggatt tgcgcctgtg ggtgctggaa gatgaaagcc ggatgatcgg ttcgaatcac
     6901 ctgccggaat gtctgcgcga gcgaatgact caggcggcga ttgcggtggt agaagatccg
     6961 tttgagatcc gtcttgaacg cctgaatgaa gagtatttct tgcgtatgca tcatgatttt
     7021 acccacgctt atggcgacga acagggctgg caggaatatt gcgaatacct gcatcacgga
     7081 ctttcggcga ttaagcgtcg gctggggtta cagcgctata acgaactcgc tgcccagctg
     7141 gatacggcgc tgacaacgca actcaccacc ggcagcaccg acggtcatct ggcctggctg
     7201 gtgccgttac ttaaagaata ttacgaccca atgtatcgct atcagcttga gaaaaaagcg
     7261 gaaaaagttg tctttcgcgg tgagtgggcg gaagtggcgg tatgggttaa ggcgcagtga
     7321 tgggcggtat tctgaaacgg cgattgtcgt gagaacaaaa tggttgcacc agaa
//
LOCUS       DBKEKI010000177         7359 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.179, whole genome shotgun sequence.
ACCESSION   DBKEKI010000177 DBKEKI010000000
VERSION     DBKEKI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.179"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            515..1489
                     /gene="hemB"
                     /locus_tag="ACUQPX_003806"
     CDS             515..1489
                     /gene="hemB"
                     /locus_tag="ACUQPX_003806"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HHV9058325.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1595..2446)
                     /gene="tauD"
                     /locus_tag="ACUQPX_003807"
     CDS             complement(1595..2446)
                     /gene="tauD"
                     /locus_tag="ACUQPX_003807"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HHV9058326.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2443..3270)
                     /gene="tauC"
                     /locus_tag="ACUQPX_003808"
     CDS             complement(2443..3270)
                     /gene="tauC"
                     /locus_tag="ACUQPX_003808"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HHV9058327.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3267..4034)
                     /gene="tauB"
                     /locus_tag="ACUQPX_003809"
     CDS             complement(3267..4034)
                     /gene="tauB"
                     /locus_tag="ACUQPX_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HHV9058328.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4047..4997)
                     /gene="tauA"
                     /locus_tag="ACUQPX_003810"
                     /pseudo
     CDS             complement(4047..4997)
                     /gene="tauA"
                     /locus_tag="ACUQPX_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
     gene            complement(5241..5756)
                     /locus_tag="ACUQPX_003811"
     CDS             complement(5241..5756)
                     /locus_tag="ACUQPX_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058329.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAVARAWGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQKTQSDLSQGDQLTEMLSFLTSEER
                     EALTNMLARKGVETVLRLLDKETQELLQLEGCKRLAALMLVDMPDEQVREILEEIEAS
                     ILSESVNAKAG"
     gene            5950..6201
                     /locus_tag="ACUQPX_003812"
     CDS             5950..6201
                     /locus_tag="ACUQPX_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9058330.1"
                     /translation="MRQREDSGRDWPSLMIQQALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            6203..>7359
                     /locus_tag="ACUQPX_003813"
     CDS             6203..>7359
                     /locus_tag="ACUQPX_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HHV9058331.1"
                     /translation="MQSYVTVNDLLGVPGMPATAKGIRQALQRFSRALGDVSRRRAGT
                     KAIEYHIDCLPEVTQKALRERYVEQLVATENNVSEVKTVTRTARNPDAVQAVEAYRGS
                     PQLMEERLNALTENQRRVSEARAALAVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAINANAKRGTNRTISRDPLYQWVLKYNRSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQANGRPMSEAYEDFVTEWQRRYADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCVHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETAD"
BASE COUNT         1815 a         1952 c         1835 g         1757 t
ORIGIN      
        1 ctcctcaggg acgtaataag ttgatccatc aattatcaat gcatagaaaa catattatgt
       61 ttcgcgctaa cgattatgct gcgttacaga cgaatggttc aattattctt tcccctaagt
      121 ccgaatgtgg acatatataa tttatggaga aaaacacgca tcaaattgat aaaaggacga
      181 tatttcagca tgttgcagat aatgttattt ttgatacata cgcgttataa atatcaaaaa
      241 cacaaaccag ataaatatct tatcaatata caacaaagtt tgaatcattc aatttatagc
      301 agcacaacca aacgccaaaa aataataacc aagagctata aataaagatt aaaaatattt
      361 tcagtgaata tgaaattatt gctatttcta ttacaaagat gtcctggtct ctctttcatt
      421 ctcttcttgt caacatcgcg acaactttcg taaaacactc ctgccccgct tcaggtatac
      481 tgtgcacctt aattccacaa acatcaggca gaccatgaca gacttaatcc aacgccctcg
      541 tcgcctgcgc aaatctcctg cgctgcgcgc tatgtttgaa gagacaacac ttagccttaa
      601 cgacctggtg ttgccgatct ttgttgaaga agaaattgac gactacaaag ccgttgaagc
      661 catgccaggc gtgatgcgca ttccagagaa acatctggca cgcgaaattg aacgcatcgc
      721 caacgccggt atccgttccg taatgacttt tggcatctct caccataccg atgaaaccgg
      781 cagcgacgcc tggcgggaag atggactggt ggcgcgtatg tcgcgcatct gcaagcagac
      841 tgtgccagaa atgatcgtca tgtcagacac ttgtttctgt gaatacactt ctcacggtca
      901 ctgcggtgtg ctgtgcgagc atggcgtcga caacgacgcg actctggaaa atttaggcaa
      961 gcaagccgtg gttgcagctg ctgcaggcgc agacttcatc gccccttccg ccgcgatgga
     1021 cggccaggta caggcgattc gtcaggcgct ggacgctgca ggctttaaag atacggcgat
     1081 tatgtcgtat tcgaccaagt tcgcctcttc cttttatggc ccgttccgtg aagctgccgg
     1141 aagcgcatta aaaggcgacc gcaaaagcta tcagatgaac ccaatgaacc gtcgcgaggc
     1201 gattcgtgag tcactgctgg atgaagccca gggcgcagac tgcctgatgg ttaaacctgc
     1261 tggagcgtac ctcgacatcg tgcgtgagct gcgtgaacgt actgaattgc cgattggcgc
     1321 gtatcaggtg agcggtgagt acgcgatgat taagttcgcc gcgctggcgg gtgctataga
     1381 tgaagagaaa gtcgtgctcg aaagcttagg ttcaattaag cgtgcgggtg cggatctgat
     1441 tttcagctac tttgcgctgg atttggctga gaagaagatt ctgcgttaat ttttttcctc
     1501 tcagaaaggg ggagttaacc tgttgaaagt acggtgaacg ccccctcacc ctaaccctct
     1561 ccccaagggg gcgagggaac cgtccgcact cgtatcaccc cgcccgataa aacggtttat
     1621 ccccaaggat cgtcgcccga tgcataatcc gccgctgtgg caggtaatcg gcattggcat
     1681 agtgctgagt cacgcggtta tcccaaatcg caatatcatt cggttgccag cgccaacgca
     1741 cctgaaactc cggtttagtg atatgggcga ataaaaagcc taacaaggct tcgctctctt
     1801 tctcgctgac atcgacaatt cgcgtagtaa agccttcatt cacaaacagc gcctgtttac
     1861 cgctcaccgg atgcgttcgc accaccggat gtagcaacgg aggatttttc gcgaccgcct
     1921 cgcgccagcg ttgatgttcc tcctcggttt tgcggtattt gtattccggg aatgatttac
     1981 ggaaatcatg ctccgcacgc agccagctca gcagctggcg gaagggaaca gagagcgcct
     2041 cataggccgc aataccgctg gtccagagcg tatcaccgcc ggtcgaaggt aactctttag
     2101 ctgccagaat cgcccctgcg ggtggcgttt caataaatgt cacatcggta tgccagttgt
     2161 cgttatccgg cggattatcg ttatgggtat ccagcacgat gatctcgtca accccttcgg
     2221 catgagggta aacagggtga atatgcagtt cgccaaaacg ctgggccagc gcgcgttgct
     2281 gctgcggcgt aatagcttga tcgcgcagaa ataccacctg atggcgcagc accgcatggt
     2341 aaagctgttc aaactgatta tcgcttaacg ggcgcgtcag gtcggcaccc gaaatttgtg
     2401 cgccgatata cggccccagc ggggtaatgc tcagacgttc actcattgta cttctccatg
     2461 ccagggcgtc agacggcgct gtaacgcgcg cagacccagt tctaaaagaa aggcgataat
     2521 cgcaatcacc gcgatccccg ccagtaccac gtcagtcgcg agaaattcac ccgctgactg
     2581 aaccataaat cctaaaccgc gtgtcgcggc aatcagctcc gccgctacca gcgtagacca
     2641 gcccacaccc agaccaatgc gtaatccggt gaggatttcc ggcagcgcgc cgggcaaaat
     2701 gacaaaccac agcacctgcg cacggctggc acccagcgac tgggcggcac gaatgcgaac
     2761 ctgctgcgcg cttttcaccc ctgccagcgc cgacatcgcc accggcgcaa aaatcgctaa
     2821 atagatcagt aagatcttcg aggtttcacc aataccaaac cagatcacca tcagcggcaa
     2881 ataagccagc ggcggcaccg gacgataaag ttcgattacc ggatccagaa tgccgcgcac
     2941 cgtcgggctt agccccatgg cgatcccgac cggaataccg atcaccaccg ccgcgagtag
     3001 cgccagcaca atgcgcgtca ggctggctgc cagatgctgc cacagcgtgg cgtccataaa
     3061 tccctgcggt ccggcaatgg tgagcagttt tgccagtact tgttgcggcg gcggcaaaaa
     3121 cagcgggcta atcagttgca gcgccgccac cgtccaccat acagtgagta aaaccgctaa
     3181 cgtgccaatg cttaaggtca cctgacgcga gagcggccag cgccatttca gccgccgcga
     3241 atgcagtttt tcattaatga gcacactcat gagaacgcct cccgttgctc aaatacgcgg
     3301 cttaaaacat attcgcgcat ggcgatgaat tgtggatcgg acttgatgct gcggctcgac
     3361 tctcccgcaa caaagcggcg agcaaagttg agcggcagcc gctccagcac acggccaggg
     3421 ccggatgaaa gcagaaccag ttcagtcgcc ataaacaccg cttcttctat atcgtgggta
     3481 atcaacagca cctgcttgcc cgtctcctgc cagagtttca gcagcagagt ttgcatctgg
     3541 tcgcgggtga aggcgtccag cgcaccaaac ggttcgtcga gtaataacag ctggggattc
     3601 gccgccagcg cgcgggcaat ccccacccgc tgacgttgac catcggaaag ctgccagata
     3661 tagcgttttt ctgcgccttc cagccccact tttttcagca tctggtgcgc gatttccagt
     3721 cgctgcattt tctctatacc tgccaattgc aggccgaacg ccacgttgtc ctgtacattg
     3781 cgccacggta gcagcccttc attctgaaaa actacgccac gctctgctcc cggtccctca
     3841 atacgcttac ccgccagttg aatgctgcca tgctgataag gcacaaaacc ggcaatcaga
     3901 ttcagcaggg tggttttacc gcagccggac ggccccagca ccaccagtag ctcgccgctt
     3961 tccagcgtca ggttgatatc ttccagtgcc ggtttgccgc cataatcggc atagagatga
     4021 gagatttgca gcatctgcgc ctccttttat tgcacgaagc gcgaggtaac gtactggctg
     4081 taatcattcg ctacagccgg gaccttgccc tgctctttca aaaactgcgc ggtgtcgatg
     4141 attgctttgt tcaccggtcc ggtcagttct gccgtttgtt gctgcggcgt cagataggta
     4201 tttcccttca ccagccccgg aacgtcacct tcaggcacgc cgcttaaacg cgccagtttg
     4261 ctgatgtttt ccggctgttt cagccacgcg tcagggttag caatgtacgg ttgctgtgca
     4321 tcgatggcgc ttttggcgaa cgctttcacg acctcaggat gtttctcggc aaaatctttg
     4381 cgcaccaccc agacgtccag cgttggcgca ccccactgcc cgacctgttc agaatcggtc
     4441 agcaccttgc cgtctttttc cagggcgtta accgccggtg cccagacata agcaccatca
     4501 atatcacccc gctgccatgc ggcgataatc gcgggcggct gcaggttcac aatctccact
     4561 agccctggtt taatccccca gtgtttcagc gccgccagca ggctgtagtg ggtggtggag
     4621 ataaacggta cggcaatgcg tttaccaatc aaatcttcgg gtttgctgat agttttcttt
     4681 accaccagcg cttcggagtt acccagtttt gacgccagca agaagacttc aatcggcacc
     4741 tgttggctgg ctgcaaccgc taatgggctg gaaccgaggt tgccgatttg cacgtcgcca
     4801 gaagccagcg cccgcacgat gctggctccg ctgtcaaact tacgccagtc cacgctcgcc
     4861 ccgctctctt tagcaaaggt gttgtcggcc tgagccactt tcgccggttc ggctgaggtt
     4921 tgatacgcca cggtgacgtt caccgcctgt gcctgaaaag cgatgaatgc cagtgcggca
     4981 agaagtgtgt tatatgacta ggtaatttat tcaagataac cgggaatggg taaaaaaaga
     5041 cgggatttga tgggattcaa taagttatgc gccgattgca ttcaagataa aacgagttaa
     5101 aaatgctcaa tgcttcgata tcgataaaac attcaagata ggcgcgccgt gtattttaat
     5161 tcttttaaga ctcttcaaaa atgtcatcgt agcctttaaa aacgaatttt aaaggctacc
     5221 ctaaaggcag gtcaatgaag ttaacccgcc ttcgcgttta cactctctga gagaatacta
     5281 gcttcaatct cttccaaaat ctctctgact tgctcgtcag gcatatcaac gagcatcaac
     5341 gctgccaggc gtttacagcc ctctagttgc agtagctctt gagtttcctt atctaacaag
     5401 cgtagcacag tctcaacacc tttccttgcg agcatgtttg taagggcttc tcgttcctca
     5461 ctggtaagaa acgacaacat ttctgttagc tgatcccctt ggcttaaatc actttgtgtt
     5521 ttttgtgttt ttggtggatt cccgttaccg gtttttagcc attcaagact aacaccttca
     5581 ctttctgcaa tgcgtaccaa tatgctttct cgaggttctg gtgtatttcc ctttttaaaa
     5641 tagctttgta acgtattgat atttatgccc caggctctgg cgacagcact tcttgatgga
     5701 taccgcctaa taagataagc catgcgctcc acgataggtt cttttgcctg ctccataaaa
     5761 gtacctctag gtatttttct gtataaaggt tcttttccat ctatctaatt gaatggaaag
     5821 gtaattttaa aaatccaaat aaaatacaaa aaaaatacct tgtattggtg ctttttgtga
     5881 tctgcgattt tatttactcg gatatccacg cgaatatccg cgcgggtaat tttgtagggt
     5941 aaaggaaaga tgagacaaag agaagatagt ggccgcgatt ggccttcgct gatgattcag
     6001 caagcactca aagataatgg gtggtcttcc tttcgggagc tttcactcca tgccggttat
     6061 gctcaggatt cactgaaagc ggtagttcgt acgccaaaca aaaaatacga acgtgttatt
     6121 tctgacgcta taggtgtacc gccagaaatc atctggccca gccggtatcg taacgatgga
     6181 tttatcagga aggcgtcgtg atatgcagtc gtacgttacc gtaaatgacc tgctcggtgt
     6241 tcctggaatg ccagccaccg cgaaaggcat ccgacaggcg ttacagcgtt tttctcgtgc
     6301 tcttggtgac gttagtcgcc gtcgagcagg tacaaaagcc attgagtacc atatcgattg
     6361 cctgcctgag gtaacccaaa aggcactgcg tgaacgctat gtggaacaac tggtggctac
     6421 agagaacaac gtctccgaag tgaagaccgt taccaggacg gcgcgtaacc ctgatgctgt
     6481 tcaggcagtt gaagcatatc ggggttctcc gcaactaatg gaagaacgcc tgaatgccct
     6541 gactgaaaat cagcgccggg tgtctgaagc aagagcggcg ctggcagtgg aagtgctgaa
     6601 gctggaaagc gccggtaacc tcgggcgact gaaagccatt aactttctgg ttgaaaaagc
     6661 ccgtaaaggt gagttgccgg aacgactgca acaggctgcg attaacgcta atgcaaaacg
     6721 tggcactaat cgcaccatca gccgtgatcc gctttaccag tgggtactga aatacaaccg
     6781 gtcgcagaac gccgcagaac gactgttgtt actggcacca ggaaaacgcg acgaaataaa
     6841 accggaagaa attagctggc tgcctgaatt tctggcgcag tatcgtcagg ctaacggcag
     6901 acctatgtcg gaagcctacg aggattttgt cacggaatgg caacgtcgct atgcggacga
     6961 accgtacatg ctggaggtga tgccttccta tgatgttgtc cggtatgcca tgaaaaaatt
     7021 gccggaagtg gtgaaacaaa aaggacgcgt taccggcagt gaataccgcc agcttgaggg
     7081 attcacccgc cgcgactgga cagctatgcc ggtgaattat gtctggattg gtgacggcca
     7141 cggtatgaag ctgaaatgtg tgcatccgat ccacgggcgt ccattttcac cggaagtcac
     7201 gtttgttatc gacggtggaa cccggtttgt tgttggctgg agtcttgatc ttgcggagaa
     7261 cgtgtttgct gttgccgggg cgatacagca cggaattcgc aatcacggaa aacccttcct
     7321 gtattactcg gataatggtt ccggtgaaac ggcagatat
//
LOCUS       DBKEKI010000178         7251 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.180, whole genome shotgun sequence.
ACCESSION   DBKEKI010000178 DBKEKI010000000
VERSION     DBKEKI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.180"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1611
                     /locus_tag="ACUQPX_003814"
     CDS             <1..1611
                     /locus_tag="ACUQPX_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HHV9058332.1"
                     /translation="AIATQHDDGYAASRINASGRMLINGSVLSKGGLINLDMHPGSVW
                     TGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFASHGSTAGTFTTLNV
                     ENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRNQGSEATTGNEVLT
                     VVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPTPNPEPTPAPAQPP
                     IVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQSKDGNIW
                     LRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIGSTHASPDYSGG
                     DGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNGVNANGTANGMSIS
                     LEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLLGRASMIL
                     GYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGWNNGVGVSAQYNKE
                     HTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(1711..3630)
                     /gene="dhaR"
                     /locus_tag="ACUQPX_003815"
     CDS             complement(1711..3630)
                     /gene="dhaR"
                     /locus_tag="ACUQPX_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HHV9058333.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            3858..4928
                     /gene="dhaK"
                     /locus_tag="ACUQPX_003816"
     CDS             3858..4928
                     /gene="dhaK"
                     /locus_tag="ACUQPX_003816"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HHV9058334.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYITRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            4939..5571
                     /gene="dhaL"
                     /locus_tag="ACUQPX_003817"
     CDS             4939..5571
                     /gene="dhaL"
                     /locus_tag="ACUQPX_003817"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HHV9058335.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            5582..7000
                     /gene="dhaM"
                     /locus_tag="ACUQPX_003818"
     CDS             5582..7000
                     /gene="dhaM"
                     /locus_tag="ACUQPX_003818"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HHV9058336.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         1833 a         1840 c         1822 g         1756 t
ORIGIN      
        1 gccatcgcaa cgcaacatga cgatggttat gccgccagcc gcatcaacgc ctcgggtcgt
       61 atgcttatca acggtagcgt tctttccaaa ggtgggctaa tcaatctgga tatgcaccct
      121 gggtcggttt ggacaggttc ctccctcagc gataatgtca atggcgggaa actggacgtt
      181 gcaatgaata acagcgtctg gaacgtaaca agtaattcta atctcgacac gctggcgctg
      241 agccattcaa ctgtcgattt tgccagccac gggtcaactg ccggcacatt taccacatta
      301 aacgtagaga acctgagcgg taacagtacc tttattatgc gtgctgatgt tgttggcgag
      361 ggtaatggcg ttaataatag aggggattta ttgaatatca gcgggagtag tgctggtaat
      421 cacgtattgg ctatccgcaa ccagggcagc gaggccacaa cgggaaatga agttctgaca
      481 gtggtaaaaa ccactgacgg cgcggcctcg ttcagcgcgt cttctcaggt tgagttgggg
      541 ggatatctgt acgatgtgcg taaaaatggc actaactggg agctttacgc ttccgggaca
      601 gttccggaac cgactcctaa tcctgaaccc acaccagctc ccgctcagcc tcccatagtc
      661 aaccccgatc ctacgcctga acccgctccc acgcctaaac ccaccacgac cgcagatgct
      721 ggcggcaatt atctcaatgt cggttactta ttgaactatg ttgaaaaccg tacgctgatg
      781 caacggatgg gtgacttgcg aaatcagagt aaagacggta atatctggtt gcgcagttat
      841 gggggaagct tggactcctt tgccagtggc aaactgagcg gctttgacat gggttacagc
      901 ggtatccagt ttggtgggga taaacgtctc tctgatgtaa tgccgttgta tgtcggtctg
      961 tatattggct caacacatgc atcgccggac tatagcggag gcgacggtac cgcacgttca
     1021 gactacatgg gaatgtacgc cagttacatg gcacaaaacg gtttttacag cgatctcgtt
     1081 ataaaagcat cgcgccagaa aaatagtttc cacgtactgg acagtcagaa caacggcgtt
     1141 aacgccaacg gcactgcgaa tggaatgagc atctccctgg aagccgggca gaggttcaac
     1201 ctgtccccta ctggttatgg gttctatata gagccgcaaa cccagcttac atacagccac
     1261 cagaatgaga tggctatgaa ggcgagtaat ggcctcaata tacatctgaa tcactacgaa
     1321 tcgctgctgg ggcgtgccag catgatactg gggtatgaca tcaccgcagg caacagccag
     1381 ctgaatgtct atgtgaagac tggcgctatc cgcgagtttt caggggatac cgaatatctg
     1441 ttgaacgact cccgggagaa gtacagtttc aaaggtaatg gctggaataa cggcgtggga
     1501 gtcagtgcac agtataacaa agagcacaca ttctatctcg aagcggatta cacgcagggt
     1561 aacctctttg atcagaagca agtcaacgga ggatatcgct tcagctttta agtgcgggat
     1621 agacaagccc ttaaacgatg ctgaaaattg ttagcgtgcg ccggaatgaa atacagatca
     1681 ctctgatgta tcagaaatcg tttctgtctt tcatacccgg cgcttaaact gccctgcatc
     1741 aatgccatgt tgcttcattt tccgccacag cgtagtgcgg ccgatcccaa gtaaagccga
     1801 catttcctga atgcgaccgc ctgtgacctg ggctgcgtta ataattgcct ctttttcaac
     1861 ttccgcaaat gacagactgg tggaaaggcg ggtggcgctg acatcatctg tcgcctgctc
     1921 ggtaaacaga tgttcaggta aatcactgac gcgaatgcgc ccgttatcac tactcagagc
     1981 aagattctcg atgacgctgt aaagttcaaa atcgttgcct ggccatgcac aagaaaccag
     2041 gcgagcgagg gcatcgtcat caattttcag ccgcgtagag aagcgttttt caagactgcg
     2101 taatttgtta ttcaccagcg ccggaatgct gccacgccgc atacgcagag gcgggatggt
     2161 aatttcaaat gcatgcagcg cgtaatacag ctggcgacta aaacgatttt gttccaccag
     2221 cattgcgagg tccgcggtcg ttgttgcaat cactttgaca tcaattggta ttaaacgccg
     2281 cgcatccagt cgcgtgataa ccccctgctt gataacctga agcaaagcag actgtaactc
     2341 caccgccaga tattcaatct tttcaagaaa cagcgtgccg ccgtgtgcca gttccagccg
     2401 actcagacgg ccattttcat tgtccgtgcg atcgccacca ataaattctt ccgccagcgc
     2461 agcatcacca tataactcac aattgacggc gatataagga cctgcagcac gctcgctttc
     2521 attatgaatt gcctgactta gcagtgcctt gcccacgccc tcttctccac aaagcaggac
     2581 aggaaagcta ctgcgcgccg cctggcgacc aaaatgaatc aagcggcggg tttgcggatc
     2641 gtcctgtggc atatgagcga aggtatggct gacttttcct aattgactgg tcattaactg
     2701 ccgcatctgt tccacaggat ggagcaacaa aataaagctg gttccctgcg tttctattat
     2761 cggtttaagg gttatcaccg catcaataaa ctggtgctgg ctttcaaagg ttgcttctac
     2821 gtgtttgagc ggatgtgcct gttttattgc ttgttgcaat acggcgggta acgttaagag
     2881 ttcagtgatt gcccgtccct gacttgccgt cgcgtcaagg cgcaagaccc gcgccgcctg
     2941 ggcattaata aattgcaaat taccctgctc gtcccagcta atcacgccat catccatact
     3001 ttctaacagg gcattaagtt gatttaaatg acggttagtt tcagcgagca aactgtccgt
     3061 cagcagtaaa ttaccgacct cgcgggcgat tgccaacgtc aacggcaaat cagctgcggt
     3121 agtttgctca accggacacg ccagcgctat tgttcccgtc aatcggccct tgctgtcaaa
     3181 caacggcgtt gcacaaaagg cccagttcca gagtgcctgt ttgaaatgtt gatcggccat
     3241 cgttttcacg gcctgaccag agatagccgc taacgatagc gcacaagtac caataattcc
     3301 ctcggcgcaa tacgtgccgt cattgaatcc cagtgcactt agctgctgca aggtttgcgg
     3361 atcgccatta cggctgagaa tgcaggcggt ttcatcgagg ataaacagcg cacactctcg
     3421 cggtgccata tattcccagg catcttccag cgcagcctgc ccgagcgtca gcatcgcttt
     3481 cttacgccga taaatagaag caaatgtcac gccctgggcc tgatgtggta cgttccatgt
     3541 ctcccgtttc atcagcttat tgcatcgctc ccaggaggtt gcaattaagg gagatatgcc
     3601 ccgaccatcg ttgttaaaag cgccactcat atcccgcatc ccttatgaca aaaggtgttc
     3661 cggctcgcaa ttttaagtat taatacacgt aaaagtttca atatggaaca ttcattaaag
     3721 catatgtttc actatgaaac ataatttggg ctaattttct tttcccttgc ctgatgcaca
     3781 atggattcat cggcagtcag tggtcgccgt gtcgttgaac atcatccatg ccctaccgta
     3841 attgctggag caaaataatg aaaaaattga tcaatgatgt gcaagacgta ctggacgaac
     3901 aactggcagg actggcgaaa gcgcatccat cgctgacact gcatcaggat ccggtgtata
     3961 tcacccgagc tgatgcccct gttgcaggaa aagtcgccct gctgtcgggt ggcggcagcg
     4021 gacacgagcc gatgcactgt ggttatatcg gtcaggggat gctttcgggg gcctgtccgg
     4081 gcgaaatttt cacctcaccg acgcccgata aaatctttga atgctccatg caaattgatg
     4141 gcggcgaagg tgtactgttg attatcaaaa attacaccgg cgatattctt aactttgaaa
     4201 cagcgaccga gttactgcac gatagcggcg taaaagtgac cactgtggtc attgatgacg
     4261 acgttgcggt aaaagacagt ctttataccg ccgggcgacg cggcgttgcc aacaccgtat
     4321 taattgaaaa actcgtaggc gcagcggcag agcgtggcga ctcactggac gcctgtgcgg
     4381 aactggggcg taagttgaat aatcaaggcc actcaatagg tatcgctctc ggtgcctgta
     4441 ccgttcctgc cgcgggcaaa ccttctttta cccttgcgga taatgagatg gagtttggcg
     4501 tcggcattca tggtgagcca ggtattgacc gccgcccctt ctcttccctt gatcaaaccg
     4561 tcgatgaaat gttcgacacc ctgctggaac atggctcata ccatcgcact ttgcgtttct
     4621 gggattatca acaaggcagc tggcaggaag aaccacaaac caaacaaccg ctccagtctg
     4681 gcgatcgggt gattgcactg gttaacaatc ttggcgcaac tccgctttct gagctgtacg
     4741 gcgtctataa ccgcctgacc acacgttgcc agcaagcggg attgactatc gaacgtaatt
     4801 taattggcgc gtactgcacc tcactggata tgaccggttt ctcaatcacc ttactgaaag
     4861 ttgatgacga aacgctggct ctctgggacg ccccggtcca caccccggcc cttaactggg
     4921 gtaaataagg agaaagcaat gtcactgagc agaactcaaa ttgttaactg gctcactcgt
     4981 tgtggcgata ttttcaacac cgagagcgag tatcttaccg ggctggatcg cgagattggc
     5041 gacgctgacc acgggctaaa tatgaaccga ggctttagca aagtggtgga aaaactccct
     5101 gctatcgcag ataaagatat cggtttcatt ctcaaaaata ccggtatgac gctgctttcc
     5161 agcgtcggtg gtgccagtgg tccgctgttc ggtaccttct ttatccgcgc cgcacaggcg
     5221 acccaggcac ggcaaagcct gacactggaa gagctttatc agatgttccg cgatggcgcg
     5281 gacggcgtaa tcagtcgcgg gaaagccgaa cctggcgata aaaccatgtg tgatgtgtgg
     5341 gtgccggtgg tggaatcgtt acgtcagtcc agcgagcaaa atctctctgt tccggtggcg
     5401 ctcgaagctg ccagtagcat cgccgaatcc gctgcacaaa gtacgattac gatgcaagcc
     5461 cgcaaaggcc gcgccagtta tctcggtgaa cgcagtattg gtcaccagga tcccggcgcg
     5521 acctcggtga tgtttatgat gcaaatgttg gcgttagccg caaaagagta aggaattggt
     5581 gatggtaaac ctggtcatag tttcacatag cagccgactg ggagaaggtg tcggtgaatt
     5641 agcccgtcag atgttaatga gtgatagttg taaaatcgcc attgccgcgg gaattgacga
     5701 tccacaaaat cccattggta ccgatgccgt caaagtgatg gaggccatcg aatctgttgc
     5761 tgatgccgac catgtgctgg tcatgatgga tatgggtagc gcattattga gtgctgaaac
     5821 tgcgctggaa ttgctggctc ccgagatcgc cgcaaaagta cgtttgtgtg ctgcgccgtt
     5881 ggtcgaaggt acactggcag caacggtcag cgcggcctcg ggggcggata tcgacaaagt
     5941 tatctttgac gctatgcatg cgctggaagc caaacgtgaa caactgggtt taccgtcctc
     6001 cgacactgaa atctctgaca catgtcctcc gtacgatgaa gaagcccgtt ctctggcggt
     6061 ggtcataaaa aaccgtaacg gcctgcatgt acgtccggcc tcccggctgg tttatacctt
     6121 atcgacattt aatgccgata tgttgctgga aaaaaacggc aaatgcgtca caccagagag
     6181 tattaaccag attgcgttac tacaagttcg ctataacgat acgctgcgcc tgattgcgaa
     6241 agggccagaa gctgaagagg cactgatcgc tttccgtcag ctggctgaag ataactttgg
     6301 tgaaacggag gaagtcgctc cacctactct gcgtcccgtt ctgcctgttt cgggtaaagc
     6361 cttttattat caaccagttt tatgtacggt acaggcaaaa tcaaccctga ccgcggaaga
     6421 agaacaagat cgattacgcc aggctattga cttcacgtta ttagatctga tgacgttaac
     6481 agcgaaagca gaagccagcg ggcttgacga tattgccgca atcttttctg gtcaccatac
     6541 actgttagat gatccggaac tgctggcggc ggcaagcgaa ctccttcagc atgaacattg
     6601 cacggcagaa tatgcctggc agcaagttct taaagaactt agccagcaat accagcaact
     6661 ggatgatgaa tatctacaag ctcgctatat tgatgtggac gatcttctgc atcgcaccct
     6721 ggtccacctg acccaaacga aagaagaact cccgcagttt aactcgccaa ctattctact
     6781 ggcggagaac atttatcctt ccacagtact gcaactggat ccggcggttg taaaaggtat
     6841 ctgccttagc gccggaagtc cggtatccca cagcgcccta atcgcccgtg aactggggat
     6901 tggctggatt tgtcagcagg gtgagaaact gtatgcgata caaccagaag aaacgctaac
     6961 gctggacgtt aaaacgcaac gtttcaaccg tcagggttaa gccaccgttc ggatggcatc
     7021 gttctgatgt catccggcaa tttacccagc actgatgttt tttaactacc tgttatattt
     7081 tttaccaatt tatgatgatt tttcttcgct aatcgcgaac caaatgtcgc tggctggcta
     7141 ataaacagac gttatttgat cactctattt cgccgttatt tatcccttta attccctttt
     7201 ctaaaatgcc tgaccgttcg cagaatgaga tttcgatcat gcagctagtg c
//
LOCUS       DBKEKI010000179         7131 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.181, whole genome shotgun sequence.
ACCESSION   DBKEKI010000179 DBKEKI010000000
VERSION     DBKEKI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.181"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(32..1672)
                     /gene="pgm"
                     /locus_tag="ACUQPX_003819"
     CDS             complement(32..1672)
                     /gene="pgm"
                     /locus_tag="ACUQPX_003819"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HHV9058337.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLSFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(1698..2243)
                     /gene="seqA"
                     /locus_tag="ACUQPX_003820"
     CDS             complement(1698..2243)
                     /gene="seqA"
                     /locus_tag="ACUQPX_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HHV9058338.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            2428..3192
                     /gene="ybfF"
                     /locus_tag="ACUQPX_003821"
     CDS             2428..3192
                     /gene="ybfF"
                     /locus_tag="ACUQPX_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HHV9058339.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            3332..3625
                     /gene="ybfE"
                     /locus_tag="ACUQPX_003822"
     CDS             3332..3625
                     /gene="ybfE"
                     /locus_tag="ACUQPX_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HHV9058340.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            3765..4295
                     /gene="fldA"
                     /locus_tag="ACUQPX_003823"
     CDS             3765..4295
                     /gene="fldA"
                     /locus_tag="ACUQPX_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HHV9058341.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            4505..4591
                     /gene="uof"
                     /locus_tag="ACUQPX_003824"
     CDS             4505..4591
                     /gene="uof"
                     /locus_tag="ACUQPX_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HHV9058342.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            4584..5030
                     /gene="fur"
                     /locus_tag="ACUQPX_003825"
     CDS             4584..5030
                     /gene="fur"
                     /locus_tag="ACUQPX_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HHV9058343.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(5114..5440)
                     /gene="chiQ"
                     /locus_tag="ACUQPX_003826"
     CDS             complement(5114..5440)
                     /gene="chiQ"
                     /locus_tag="ACUQPX_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HHV9058344.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(5477..6883)
                     /gene="chiP"
                     /locus_tag="ACUQPX_003827"
     CDS             complement(5477..6883)
                     /gene="chiP"
                     /locus_tag="ACUQPX_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0052778 - diacetylchitobiose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin ChiP"
                     /protein_id="HHV9058345.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         1729 a         1824 c         1777 g         1801 t
ORIGIN      
        1 cttgcgaccg cccttttttt attaaatgtg tttacgcgtt tttcagaact tcgctaacaa
       61 tctcaaccgc ttctttctca atctgcttgc gatgttcttc accgaggaag ctttcgcagt
      121 agatcttata tgcgtcttcc gtgcctgacg gacgcgcggc gaaccagccg ttgtcagtca
      181 tcactttcag accgccaata gaagcaccgt tgcccggagc agcagtcagg cgcgcggtga
      241 tcgggtcacc tgccagggtg ctggcgctca ccatttccgg agacagctta gacagcgccg
      301 ctttttgtgc ggaagtcgca gctgcctgca aacggttgta gctcggcgca ccaaagcgtt
      361 ttgccagttc gttgtagtgt tcctgcgggt tcttaccggt gacagcggtg atttccgccg
      421 ccagcagaca cataatgatg ccgtctttgt cggtggacca cggcgtgccg tcgaaacgca
      481 ggaaggaagc ccctgcgctc tcttcgccgc caaagccgaa gctgccgtcg aacagaccat
      541 caacaaacca tttgaaacct accggtactt ctaccagctt acggcccaag tcattgacca
      601 cacggtcgat catcgcagaa gaaaccagcg ttttaccgac agcaacatct ttgccccact
      661 gcggacgatg ctggaacagg tagttgatcg ccaccgccag gtagtggttc ggattcatca
      721 aacctgccgg agtgacgata ccgtgacggt cataatccgg gtcgttagca aacgacagat
      781 cgaacttatc acgcagtgcc agcagacccg ccatcgcaca ctcggaggag cagtccatac
      841 ggatcgcacc gtctttatcg aggtgcataa agcggaaggt ttgatcgacc tgatcgttaa
      901 cgatggtcag gttgaggttg taatactcgc caatgcgctt ccagtattcg ataccggaac
      961 cgcccaacgg atcaacgccc agcgtcaggc ccgctttctg aatcgccgcc atatcaacga
     1021 tatcggccag cccttccacg aacggctgca ccagatcctg ctctttcaca tggccggatg
     1081 ccatcgcttc gtcgagggag atacgcttca cgcctttcag gccatcggcc atcagtgcgt
     1141 tggccctgtc ttccaccact ttggtgacgt tagtatcagc cgggccacca tttggcggat
     1201 tgtacttgat accaccatct tccggcgggt tatgggacgg tgtaatcacg ataccgtctg
     1261 ccagcgggcc acctttttta ttgtgaacca ggatggcatt ggaaacggca ggcgtcggag
     1321 tgaagccatt gttttcctgc acaatgacat caacgccgtt cgctgccagc acttccagta
     1381 cggaaatgaa cgcaggctcg gacagggcgt gagtatcttt acccacatag caagggccag
     1441 tgatgccgtt tttcgcacgt tcttcagcaa ttgcctgagc gatcgccaga atgtgcggct
     1501 cgttaaagct gtggcgcgct gcactgccac ggtgaccgga agtaccgaat ttcaccgcgt
     1561 gctccgcatt ccctgcttct ggtttcagta cataatattg cgccgtcagt tgggcgacgt
     1621 taatcaaatc actctgttgt gcaggttggc ctgcacgatt gtggattgcc attgctttgt
     1681 cctttgtctg caacgtttta gatagttccg caaaccttct caatcaattc cgccgggaat
     1741 tgcatcgact gcatgatgtg ttcgatcatg ctgcatttac ggccggtgtt ggtgttggtg
     1801 atcacccaat acggcgtgcc tggcacatgt ttcggcttgg tctgattacc atttttcagc
     1861 agcgtttgtt catctgccgc aaagtacacg cgtgtacgac cgtgcaacga ttccgttgct
     1921 tcggcaaacg cctgggcgtc aagagaatat agtgtagaca acagcagcat aaagcgattg
     1981 accgctcgct tttgctctgc gtattcgtcc gaaagcagaa gttcacgcat tgcgcgaacc
     2041 ttgtctttaa tcgttttgac cggcttcgct tcgacgatag caggtgacgc aacgcgaacc
     2101 tctttcgtca ccggagcagc aggctgtgat gcggcggaaa atttcaacat acgccgtaaa
     2161 atgtcggatg cgctctcgcc gatatgctta gtgtggctgg caatatagct gtagagttca
     2221 tcatcaactt caatcgtttt catcttaatc cagtgcagtg tcttagctga atacaagtcg
     2281 ccaagagtat atgaataaat cccggcagcg gatagcgtca agcctggctt gcagaaaaac
     2341 tcggaataaa ccttatttgt tgcagccctg tggcacaatg gtcaacataa taccctaacc
     2401 cgacaacgcg aaaaaaagaa ctttgccatg aaattaaata tccgcgcgca aactgcacaa
     2461 aaccagcaca ataattctcc catcgttctt gtccatggtc tgtttggtag cctcgacaac
     2521 cttggcgtac tggctcgcga tctggtaaac gatcacaata tcatccaggt tgatatgcgt
     2581 aaccacggtc tttcaccgag agagccggta atgaattacc cggcgatggc gcaggatctt
     2641 gttgataccc tggatgcact gcagatcgac aaagcaacat ttatcggtca ctccatgggc
     2701 ggtaaagcgg taatggcact tactgcactg gcccccgatc gcatcgataa actggtggcg
     2761 atcgatatcg caccggtcga ctatcacgta cgccgtcatg atgagatttt cgcagctatc
     2821 aacgcggtca gtgaatcgga cgcacaaact cgccagcaag cagcagcaat aatgcgccag
     2881 catcttaatg aagaaggggt gattcagttt ctgttgaaat cttttgttga cggggagtgg
     2941 cgctttaacg tgccggtatt gtgggatcag tatccgcata ttgtgggttg ggagaaaatc
     3001 ccggcatggg atcaccctgc cctgtttatc cctggcggca attctccgta tgttagcgag
     3061 cagtaccgtg atgatttact ggcacaattt ccacaggcac gggcgcatgt gattgcaggc
     3121 gcgggtcact gggtccatgc tgaaaaaccg gatgcggtat tacgcgctat ccgtcgctat
     3181 ctcaatgatt aacgcaactg attaaaaacc cagcgtccgc gtcgctttcc gcgcgtgggc
     3241 gttggcgtga ctcatcgcgc tgatgtatta tggcgcgcta tccatccggg cagaggcctg
     3301 gctgattgtt tcccccgaag tcaccaagat catggccaaa gaacaaacgg accgtacgac
     3361 attagatctg ttcgcgcacg agcgtcgacc gggacgaccg aaaactaatc cgctttcgcg
     3421 cgatgaacag ctgcgtatta ataaacgcaa ccagctaaaa cgcgacaaag tacgtggcct
     3481 taagcgtgtc gaactgaagc tgaacgcgga agctgtcgag gcgctgaacg agctggcgga
     3541 atcgcgtaat atgagccgta gcgaactgat cgaagagatg ttaatgcagc aactggcggc
     3601 gctgcgtagt cagggcatcg tttaaatttc cactttcatg tagcacagtg tgcagtcctg
     3661 ctcgtttgct gattccgcaa gactgcctgt tctgctatga ttgcctttat ccgtgggcaa
     3721 ttttccaccc ccatttcaat aagtttcaag aggttatttc actcatggct atcactggca
     3781 tctttttcgg cagcgacacc ggtaataccg aaaatatcgc aaaaatgatt caaaaacagc
     3841 ttggtaaaga cgttgccgat gtccatgaca ttgcaaaaag cagcaaagaa gatctggaag
     3901 cttacgacat tctgctgctg ggcatcccaa cctggtatta cggcgaagcg cagtgtgact
     3961 gggatgactt cttcccgact ctcgaagaga ttgatttcaa tggtaaactg gttgcgctgt
     4021 ttggttgtgg tgaccaggaa gattacgccg aatatttctg cgacgcattg ggcaccatcc
     4081 gcgacatcat tgaaccgcgc ggtgcaacca tcgttggtca ctggccaact gcgggctatc
     4141 atttcgaagc atcaaaaggt ctggcagatg acgaccactt tgtcggtctg gctatcgacg
     4201 aagaccgtca gccggaactg accgctgaac gtgtagaaaa atgggttaaa cagatttctg
     4261 aagagttgca tctcgacgaa attcttaatg cctgatgtga tgcggcgtag actcatgtct
     4321 acgccgtatt aatagataat gccaatcaaa ataattgcta caaatttgta acttttgctg
     4381 ttgtacctgt acaatgtccc agtgttcaag tggccttgcc gttgtaaatg taagctgtgc
     4441 cacgttttta ttaacaatat ttgccaggga cttgtggttt tcatttaggc gtggcaattc
     4501 tataatgata cgcattatct caagagcaaa ttctgtcact tcttctaatg aagtgaaccg
     4561 cttagtaaca ggacagattc cgcatgactg ataacaatac cgccctaaag aaagctggcc
     4621 tgaaagtaac gcttcctcgt ttaaaaatcc tggaagttct tcaggagccg gacaaccatc
     4681 acgtcagtgc ggaagattta tacaaacgtc tgatcgatat gggtgaagaa attggtctgg
     4741 ctacggtata tcgcgtactg aaccagtttg acgacgctgg tatcgtcacc cgccacaatt
     4801 ttgaaggcgg taaatccgta tttgaactga cacagcaaca tcaccacgat cacctgattt
     4861 gcctcgactg cggcaaggtt atcgaattta gtgatgattc catcgaagcg cgtcagcgtg
     4921 aaattgccgc aaaacatggc attcgcctga ctaaccacag tctctatctt tacggtcact
     4981 gtgccgaagg cgattgccgc gaagatgagc acgcgcacga aggcaaataa gccagcctga
     5041 acgagaaaag ccaacctgcg ggttggcttt tttatacaag ggaaataaga aaagcgagag
     5101 ttacagctct cacttatttg ttattactgc gtatttcctg ccagacctta tcacaatcgg
     5161 ctttcaccgc ctgatcattt ccggtttgcg ttgcacgcag acactgctga taatccagta
     5221 cgcgtacact ttcctggtcg gcaaactgct gatgttgttt ctctttcttc agcacgttta
     5281 acacgctctg gcaggcttca attttttctg gcgaaccttg cgcggtgttg atacaggcgc
     5341 tgtatgcctc tttcagacgg ctgtcttcct gtggtgcggt tgattgcgca caagctacca
     5401 gccccgatgc catcatggcg atgagaatca gttttttcat tgaatgcggc aggcacaaaa
     5461 cctgccgcgt cgggcatcag aagatggtga atggtgcgat taccataaat ttcacgtcac
     5521 gctcatcctg gaagatgtta ccgtaaccgc cgccccagct tgggatgtcg gagtggttgt
     5581 cgtattcggt gaagtgcagt ttgaacatcg tgcctttggc gcgaccgtcc tgaatggtgt
     5641 agaccgcatc caggctgtag gcagactctt caatagtccg gtttttgtcg tagtacgcat
     5701 ccggattgct ctgccaggtc gcaggtttag catcccatgc gtaaacgtag gaagcgccga
     5761 tggcgaagcc tggaagattc cagtttttca ggtcatacat cgcaccgaag aagaccgctt
     5821 tttcgccgtt agcgttgaag tcggaacggt tatcccacca gatatccagg cggccgttgg
     5881 aggaagcgta ggttggggtc atacgttgca ggaagtatcc ctgctgactg tcagccttaa
     5941 cccaggtgcc ttcgaggcgc aaatccacta cgtcagccgc ccggtaacca aaggtcaacg
     6001 cttgcagcca ggcggtgccg tcataaagat cgttgacgct gcgatcgtca actttatcgc
     6061 gggtaccata gaactggtag ctggtggtta acgggctacc ggcgatatca aatttgtagc
     6121 tggctttggc aaaatattga tcgatatacc cttccgcctg accaaatgcc gcttccagta
     6181 cgaagttatt tttgaagtcg tatttcgcac cgatggagtg cagataatca actttggtgg
     6241 ttttatcgtt ctgataaaac tcatccattt ccagatgcca cggcgctttg tattcgttgg
     6301 tccacatgta ggagaaactc aaggcaccag catcgccgta atcaaaattc gccccggctt
     6361 ccgccccctg ataagtaccc ggcataaagc tccagtgcgg cgctaacagc gtttgaccgg
     6421 ttggctgaat gtaacctgcc ctcgcccaaa ccggaccgta tttaaatttg gccgcagctt
     6481 tatacaggct tataccgctt ttgtcgccgg accagtcttc gtcataggct ttattacttt
     6541 ttgaaaacgc gatttcgttc gggtgggagc tgtcgccgtt ttccgccatt tcaatcgccg
     6601 taaacgcagc aatatcaagg ccgaacatat cagcagcata accggactga aaatcgaggt
     6661 tggcattcca ggtggagtga gaaaggttgg ttttgtattt gtcgccgtcg gtaacatctt
     6721 tacggtcgcg ttcacgctgc cagtaataga taccgccggt taaggttgaa tcgtcgatga
     6781 atccttcggc gcgagcctcc ggcattgcca taaagcccga cattgctgta acaccggcaa
     6841 tagccagcgc cagcgtacta cgtttgccac taaacgtacg catgggttaa tcctctttga
     6901 cgtataaatt gctgcaccaa aggtgaacag cgaaaaaaaa taaaaattaa aaaagtagcg
     6961 ttgagaaaag tctcgctgac tacaggaaca gactattttt cgcgacgcga attatcaatc
     7021 tctttctgta accagaatgt aagaacatga gcaaccgcac atattttgat gatttttgtt
     7081 acaaggctta agtttgcggt tgaaaggctt gcagaagaat aaaaacgtga a
//
LOCUS       DBKEKI010000180         6996 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.182, whole genome shotgun sequence.
ACCESSION   DBKEKI010000180 DBKEKI010000000
VERSION     DBKEKI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.182"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..82
                     /locus_tag="ACUQPX_003828"
     CDS             <1..82
                     /locus_tag="ACUQPX_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HHV9058346.1"
                     /translation="YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            214..426
                     /locus_tag="ACUQPX_003829"
     CDS             214..426
                     /locus_tag="ACUQPX_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HHV9058347.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            713..1390
                     /locus_tag="ACUQPX_003830"
     CDS             713..1390
                     /locus_tag="ACUQPX_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HHV9058348.1"
                     /translation="MEENNTSTDKNTLTALIDYENCGSLKNISLEQYGELIIFVGPQQ
                     NVVVLPADSFPEGARITIRQVSGVSRNNVDFHLVLELGRISCCAAGKDKTYHIISSDK
                     GYDGVIRTLKEAGITCIRRDVPESIAAAGESGADKSNCLKQPSVPESDIVSRWAGKLQ
                     QIAGSGAGNMPVSVETFNNYLKSHMRDEWNKTRAVAIRNELVRRGVTEVREKKIIWKK
                     AKKENKA"
     gene            2056..2247
                     /locus_tag="ACUQPX_003831"
     CDS             2056..2247
                     /locus_tag="ACUQPX_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058349.1"
                     /translation="MNGFRNSSRNGQVWRYQSAGSRAVILEAAEAWRRAAGVAPRTDW
                     QQFARKRAEQCHRRCRGRV"
     gene            2491..2952
                     /locus_tag="ACUQPX_003832"
     CDS             2491..2952
                     /locus_tag="ACUQPX_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HHV9058350.1"
                     /translation="MMKYIPLVLFIFSWPVLSEDIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVFQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            2998..3207
                     /gene="hha"
                     /locus_tag="ACUQPX_003833"
     CDS             2998..3207
                     /gene="hha"
                     /locus_tag="ACUQPX_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HHV9058351.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            3245..3835
                     /locus_tag="ACUQPX_003834"
     CDS             3245..3835
                     /locus_tag="ACUQPX_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HHV9058352.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            4075..4323
                     /locus_tag="ACUQPX_003835"
     CDS             4075..4323
                     /locus_tag="ACUQPX_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HHV9058353.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            complement(4340..4606)
                     /locus_tag="ACUQPX_003836"
     CDS             complement(4340..4606)
                     /locus_tag="ACUQPX_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HHV9058354.1"
                     /translation="MSTERSLELFRAYNYTPRIEQQLLCGFLLAPLIFFSVAKLVEDY
                     RGFHFSGSCKSTSEPVPCHLTAWPATKFLKRSAIYHAHQKQARM"
     gene            4597..4644
                     /locus_tag="ACUQPX_003837"
     CDS             4597..4644
                     /locus_tag="ACUQPX_003837"
                     /inference="COORDINATES: protein motif:HMM:TIGR03475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058355.1"
                     /translation="MCSLLLCNISAGRCD"
     gene            4637..5494
                     /gene="repA"
                     /locus_tag="ACUQPX_003838"
     CDS             4637..5494
                     /gene="repA"
                     /locus_tag="ACUQPX_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HHV9058356.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSVLFAALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANGEAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            6410..6679
                     /gene="yacA"
                     /locus_tag="ACUQPX_003839"
     CDS             6410..6679
                     /gene="yacA"
                     /locus_tag="ACUQPX_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HHV9058357.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            6679..6789
                     /gene="yacB"
                     /locus_tag="ACUQPX_003840"
                     /pseudo
     CDS             6679..6789
                     /gene="yacB"
                     /locus_tag="ACUQPX_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
BASE COUNT         1969 a         1530 c         1816 g         1681 t
ORIGIN      
        1 gtatgcggca gcgcgtctgg ataaaatccg ccgccagaac aggataaagg cagagcttca
       61 ggccgtgctt gatgagaaat aaaaaaaagc ctccatcaga gaggaggcag ggaaataagg
      121 cttaaaagga aataacttcc gtacccgaaa ggaagccaat tcagtagatt atttaacacc
      181 ataatactga cgggttaaag aggagtgata aagatgaatg gattcagaaa cagttcacgg
      241 aacggtcagg tctggcgctg gcagcgtgcc ggaagccggg ccgttattct ggaggtcagt
      301 ggacgctgga tggaagcggc agaagcatgg cgacgggctg ccggtgttgc gccccggaca
      361 gactggcagc agtttgcccg aaaaagggct gaacactgcc accggcgcag ccggggaagg
      421 gtgtaacgaa aaaagcagcc ttcattactc cagggattca gaaggctgaa gtatgcatga
      481 gatttttctt ttttatatct gacctggcag tatgaccagt cagcattcat tatcacagga
      541 ttattgctgt attaagtaat aacgtgctgt tttgtgattg atttcataaa acaagacgtc
      601 gtttaatatg cgcctgaata ttcacgaggg aagtctttct ttttatatgg tgttcccgga
      661 aataaaatat tttgtgttat tgctcacccg gttatgaata aaaggtaaaa acatggaaga
      721 aaataatacg agtacagata aaaacaccct gacagccctt attgattatg aaaactgtgg
      781 cagtctgaaa aatatttccc ttgagcagta tggggaactg attatttttg tcgggccaca
      841 gcagaacgtc gttgttcttc ctgcagactc ttttcctgaa ggtgcgagaa tcactatcag
      901 gcaggtgagt ggtgtgtcca ggaataatgt cgattttcat cttgttctgg agctggggcg
      961 gataagctgt tgtgcagcag ggaaagataa aacataccac atcatttcat cggataaggg
     1021 atatgacggt gttatcagga cgctgaaaga agccggtatt acctgcatcc gtcgtgatgt
     1081 accggaaagt attgctgctg ccggtgagtc tggtgcagac aaaagtaact gcctgaaaca
     1141 accttctgtt cccgaaagtg atatcgtcag ccgttgggca ggaaagctgc agcagatagc
     1201 cggcagcgga gccggtaata tgcctgtgtc cgtcgaaaca tttaataatt atttaaagtc
     1261 acacatgcgt gacgaatgga acaaaacccg ggcagtggcg ataaggaatg aactggttcg
     1321 tcggggagtc acagaagtca gggagaaaaa gataatctgg aaaaaggcga aaaaggagaa
     1381 taaagcatga ggtatcgcag ctgtactcag ataaaaacag cccccgttgc ggttgcggag
     1441 cgaagggggc tgaaaagggg aattaaggat gattgttatt atggtgaacc agaaggttat
     1501 ccctgtctgt aagctgaatc ggactaaacg acataatacc tgtaacaata cctgtaacat
     1561 attttcctgg caggacaagc aggaacaata aaatgtgtga tttaattcaa aaaatgtgcg
     1621 cggcaaagaa aaaccgggaa attgcttccc cggtccggtc catttagcca gttccctgag
     1681 caggatctga tatgagcatt attcactttg ccagacatgg tgaaaaaagt gaatatatgg
     1741 aggagaaaaa catcgcctga atcacagatt cgggtgctca gcgcgtaaga agacgggaaa
     1801 gaaaagcgcc tttccgtttc agcatcggga ataatcactg aaacatcagc agtatatgtg
     1861 gtgtgtatga atatcccggc agcattaatt cttctgtgag gaaatgcatt gataagaaac
     1921 agaataagtc ggtggcaagg aaaatgaatt tattaattca gtatccgttt atcagataaa
     1981 aaagggaaag agtatggtga aactgattat ttttattatc acaataatac tgacaactaa
     2041 aggggggaca ggaaaatgaa tggattcaga aacagttctc ggaacggtca ggtctggcgt
     2101 taccagagtg ccggaagccg ggccgttatt ctggaagcgg cagaagcatg gcgacgggct
     2161 gccggtgttg caccccggac agactggcag cagtttgccc gaaaaagggc tgaacaatgc
     2221 caccggcgct gccggggaag ggtgtaacga aaaagcagcc ttcattactc cagggattca
     2281 gaaggctgaa taatgcatga gatttgcttt ttttaatatc cggtcgggca gtatgaccgg
     2341 acagttctga gtagcacaga gttttttccg tattaagtcg tgattttata ttttgtgatt
     2401 tatttcacaa aataaggtgt tattcattgt gtgctgcaat gttcaggatg cctgaaatac
     2461 aggtgccatt tatctgatat ggagaataac atgatgaaat atattccact ggtattattt
     2521 atcttttcat ggccggtatt gtctgaagat attcatggac gggttgttcg tgtactggac
     2581 ggtgatacca tcgaggtcat ggactcacgg aaggcggtgc ggatcaggct ggtaaatatt
     2641 gatgcaccgg aaaagaaaca ggattatggc cggtggtcaa cggacatgat gaaatcactg
     2701 gttgcaggaa aaacggttac cgtcacatat tttcagcgag atcgttatgg tcgtattctc
     2761 gggcaggttt atgcaccaga tggaatgaat gttaatcagt ttatggtccg ggccggggct
     2821 gcctgggtat atgagcagta caacacagat cccgtgctac ctgtctttca gaacgaagcc
     2881 agacaacaaa aacgtgggct ctggtcagat gctgatccgg ttcccccctg gatatggaga
     2941 caccgtaaat aaagcgctgg caatcttatc attcatctgt tctcagcggg aataaaaatg
     3001 gcaaaaacaa aacaggaatg gctgtatcag ttacggcgat gttcatcagt caatacgctg
     3061 gaaaaaatta ttcataaaaa cagagacagt ttattaaaca gtgaacgtga atcatttaat
     3121 tcagcagcag accaccggtt agcggaatta atcaccggaa agttatatga caggatacca
     3181 aaagagatat ggaaatatgt aaggtaataa ttcagaataa ttaattacca ggagcagata
     3241 aacaatgaag ctgattattt ttattctcat tgttctgatt attgcagcgt tacttatccg
     3301 gattatcctc agaagtgtaa atcagcattc tcctttgctg atgcagttgc atgcggcagg
     3361 tatacggacc ggtgatgcag agcgaatact gtccggcggt gaatactggc aacgtcagaa
     3421 gacgctgctg acagaaaggg aagtcagttt tatgaaagga ctgttcagaa ttgtggatat
     3481 gaagcggtgg tatctgtgtc cgcaggtacg ggtcgcggat atcgtccagc tgaacgggaa
     3541 tatccggcca cgatcacgcc agtggtggca gttattcagg atggtgtctc agtggcatgt
     3601 tgatgtggtt atcgttgagc ggcgttcgtt cagtattgtt gcagcagtag agctggatga
     3661 tgccagccat ttacgaccgg aacgcagacg ccgggatatt cttctggaag aggttctgag
     3721 gcaggctggt attccgttgc tcagaagcca cgatgccaga aaactgctgc agatgaccgg
     3781 agaatggctg aatacaacag gggctgatca gcagtccccg gaacatcgta gctgacgcct
     3841 tcgcgttgct cagttgtcca accccggaaa cgggaaaaag caagttttcc ccgctcccgg
     3901 cgtttccata actgaaaacc atactatttc acagtttaaa tcacattaaa cgacagtaat
     3961 ccccgttgat ttgtgcgcca acacagatct tcgtcacaat tctcaagtcg ctgatttcaa
     4021 aaaactgtag tatcctctgc gaaacgatcc ctgtttgagt attgaggagg cgagatgtcg
     4081 cagacagaaa atgcagtgac ttcctcatca ggtacaaagc gagcatacag aaaaggtaat
     4141 cctctgacgc ttgcagaacg tcagcaggca tccctggcaa gaaaacgagc tacacataaa
     4201 gaactcagag tgttcattcc tgctgcgtta aaagcgcaat tgcaggtgat gtgtgaagct
     4261 gagggcgtta cccaggctga gatgattgcc gaactgataa agcagaagag tgcttttagc
     4321 taactaaaat gtagcgacat cacattcttg cctgtttctg gtgtgcgtga tagattgctg
     4381 atcgtttaag gaattttgtg gctggccacg ccgtaaggtg gcagggaact ggttctgatg
     4441 tggatttaca ggagccagaa aagtgaaaac cccgataatc ttcgacaagt ttggcgactg
     4501 agaagaagat taacggggct aacaagaaac cgcatagaag ctgttgctct atgcggggag
     4561 tatagttata tgcccggaaa agttcaagac ttctttctgt gctcactcct tctgtgcaac
     4621 ataagtgcag gaaggtgtga ctgatcttca acaaacgtat taccgccagg taaagaaccc
     4681 gaatccggtg tttacacccc gtaaaggtgc cggaacgctg aagttctgcg aaaaactgat
     4741 ggaaaaggcg gtgggcttca cctcccgttt tgatttcgcc attcatgtgg cgcatgcccg
     4801 ttcgcgtggt ctgcgtcggc gcatgccacc ggtgctgcgt cgacgggcta ttgatgcgct
     4861 gctgcagggg ctgtgtttcc actatgaccc gctggccaac cgcgtccagt gctccatcac
     4921 cactctggcc attgagtgcg gactggcgac ggagtctgct gccggaaaac tctccatcac
     4981 ccgtgccacc cgggccctga cgttcctgtc agagctggga ctgattacct accagacgga
     5041 atatgacccg cttatcgggt gctacattcc gaccgatatc acgttcacat ctgtactgtt
     5101 tgctgccctc gatgtgtctg aggatgcagt ggcagctgcg cgccgcagtc gtgttgaatg
     5161 ggaaaacaga cagcgcaaaa aacaggggct ggataccctg ggtatggatg aactgatagc
     5221 gaaagcctgg cgttttgtgc gtgagcgttt ccgcagttac cagacagagc ttaagtcccg
     5281 tggaataaaa cgtgcccgtg cgcgtcgtga tgcgaacagg gaacgtcagg atatcgtcac
     5341 cctggtgaaa cggcagctga cgcgtgaaat ctcggaaggg cgctttactg ctaatggtga
     5401 ggcggtaaaa cgtgaagtgg agcgtcgtgt gaaggagcgc atgattctgt cacgtaaccg
     5461 caattacagc cggctggcca cagcttcccc ctgaacctga cctcctctga ataatccggc
     5521 ctgcgccgga ggcatccgca cgtctgaagc ccgccaacgc acaaaaaaac agcaccacat
     5581 acaaaaaaca acctcaccat ccaccttctg gtgcatccgg ttccccctgt tttcgataca
     5641 aaacacgcct tacagacggg gaattttgct tatccacatt aaactgcaag ggacttcccc
     5701 ataaggttac aaccgttcat gtcataaagc gccagccgcc agtcttacag ggtgcaatgt
     5761 atcttttaaa cacctgttta tatctccttt aaactactta attacattca tttaaaaaga
     5821 aaacctattc actgcctgtc ctgtggacag acagatatgc acctcccacc gcaagcggcg
     5881 ggccccgacc ggagctacat tagttacaac atgcaaacat agacctcgga aaaaccccgg
     5941 accagcgcag aactgaagcc acaaagcccc tccctcataa ctgaaaagcg gccccgcccc
     6001 ggcccgaagg gccggaacag agtcgctttt aattatgaat gttgtaacta tactccgtca
     6061 tcgctgtcag tcttctggct ggacgtactg aatacacgct cgtaagcggc cctcacggcc
     6121 cgctaacgcg gagatacgcc ccgacttcgg gtaaaccctc gtcgggacca ctccgaccgc
     6181 gcacagaagc tctatcatgg ctgaaaacgg gtatggctta gcaggactgg ggatgggtaa
     6241 ggtgaaatct atcaatcagt accggcttac gccgggcttc ggcggtttta ctccggtatc
     6301 acatgtaacc acagcgtgcc gccttccatg ccgctgacgc ggcatattct ggtgacgata
     6361 tctgagtcgt tatatactgt gtatatacgt agtaatgacg aggtgataaa tggcacaggt
     6421 taatatgagt ttaagaatcg acgctgaact gaaggatgct tttatggctg ctgcaaaaag
     6481 catggaccgt aatggctctc agttaatccg ggattttatg cgtcagaccg ttgaacggca
     6541 gcataatacc tggttccgtg accaggttga ggcaggacgt cagcaactcg agcacggcga
     6601 tgtacttccc catgacatgg tcgaatcctc tgctgcagca tggcgagatg aaatgagcag
     6661 gaaggttgcc ggtaaatgat ggagatattc tggaccatgc tggccagcca ggacaggaag
     6721 cgcattcgtg aatacattgc ggagcagaac ctgatagcag ccattgaact ggacgaacgg
     6781 attggttatt tcggacgaac gcaccggctt acgccggttt tacatctgta ctacatgtaa
     6841 cctcatacac acataaatga gtaacatact gattaatatg gtaaatcatg acgattgaaa
     6901 gcttttttat tggcacccgc cgctctgata aacgttacgg gcctcagagc aaggatatgc
     6961 aggtttctga attcatttct ctgatttctc ccaaaa
//
LOCUS       DBKEKI010000181         6990 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.183, whole genome shotgun sequence.
ACCESSION   DBKEKI010000181 DBKEKI010000000
VERSION     DBKEKI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.183"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..184)
                     /locus_tag="ACUQPX_003841"
                     /pseudo
     CDS             complement(1..184)
                     /locus_tag="ACUQPX_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            262..1356
                     /gene="ddlA"
                     /locus_tag="ACUQPX_003842"
     CDS             262..1356
                     /gene="ddlA"
                     /locus_tag="ACUQPX_003842"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HHV9058358.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1380..1592)
                     /locus_tag="ACUQPX_003843"
     CDS             complement(1380..1592)
                     /locus_tag="ACUQPX_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HHV9058359.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGGLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1852..2160
                     /locus_tag="ACUQPX_003844"
     CDS             1852..2160
                     /locus_tag="ACUQPX_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HHV9058360.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2219..3313)
                     /gene="yaiW"
                     /locus_tag="ACUQPX_003845"
     CDS             complement(2219..3313)
                     /gene="yaiW"
                     /locus_tag="ACUQPX_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HHV9058361.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3326..4546)
                     /gene="sbmA"
                     /locus_tag="ACUQPX_003846"
     CDS             complement(3326..4546)
                     /gene="sbmA"
                     /locus_tag="ACUQPX_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HHV9058362.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4898..6055
                     /gene="ampH"
                     /locus_tag="ACUQPX_003847"
     CDS             4898..6055
                     /gene="ampH"
                     /locus_tag="ACUQPX_003847"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HHV9058363.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6056..6559)
                     /locus_tag="ACUQPX_003848"
     CDS             complement(6056..6559)
                     /locus_tag="ACUQPX_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9058364.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6647..>6990)
                     /locus_tag="ACUQPX_003849"
     CDS             complement(6647..>6990)
                     /locus_tag="ACUQPX_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HHV9058365.1"
                     /translation="DYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKL
                     AYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQD
                     WSANVGVKYTW"
BASE COUNT         1768 a         1787 c         1683 g         1752 t
ORIGIN      
        1 aagctatgaa atatattctc ttcgttaact tataattaag agaaaaaaac tcaccgcatt
       61 gccatttaag ctaggaataa attagcttcc cgctcatcat ttttgtgatc ttgcgcacat
      121 ctttgagata acgcactttc ttcaccagta cgttgcacgc tttgtcagca gaagattgca
      181 acaatctttc agaaggccgc gaatcccccg ttttgtcgtg gtaatgtatg cactttgtgt
      241 tagacgggat aggtttttaa gatggaaaaa ctgcgggtag gaatcgtttt tggtggtaaa
      301 tcagcggaac atgaagtgtc tctgcaatcg gcaaaaaaca ttgtcgatgc cattgataaa
      361 agtcgcttcg acgttgtgct gctgggcatt gataaacaag ggcaatggca cgtcagcgat
      421 gccagcaatt atctgctaaa tgcagacgat cctgcccata ttgcgttgcg cccttcggcg
      481 accagccttg cgcaggtgcc aggtaaacat gagcatcagc ttatcgacgc gcaaaacggt
      541 cagccgttgc cgacggtgga tgtcattttc ccgattgtcc acggtacgct gggcgaagat
      601 ggttccttgc agggaatgct gcgggtcgcc aatttaccgt ttgtaggttc tgatgttctg
      661 gcttcagcag cctgtatgga taaagatgtc accaaacgtc tgctacgcga tgccgggctg
      721 aacattgcgc catttattac cctgacgcgc gctaatcgtc acaacatcag ttttgccgaa
      781 gtggagtcta aactggggtt accactgttt gtaaaaccgg ctaatcaggg ctcttctgtt
      841 ggtgtcagca aagtaaccag tgaagaacag tacgcaattg ccgtcgatct ggcgttcgag
      901 ttcgatcata aagtgatcgt tgagcaaggg atcaaaggtc gtgagatcga atgcgcagtt
      961 ctgggcaacg acaatccgca agccagcacc tgtggcgaga tcgtactcac cagcgatttc
     1021 tatgcctacg acaccaagta cattgacgaa gatggcgcga aagtggtagt tccggcagcc
     1081 attgcaccag agatcaacga taagatccgg gcgattgccg ttcaggctta tcagacgttg
     1141 ggatgcgcag gcatggcgcg tgtagacgtg tttttaaccc cagagaacga agtggtgatc
     1201 aacgagatca acacactgcc tggcttcacc aacatcagta tgtatccgaa gctgtggcaa
     1261 gccagcggtc tgggttacac cgatctgatc acacgtttga ttgagctggc gctggagcgt
     1321 cacgctgcgg ataacgcatt gaaaaccaca atgtaatatt ttaacgcccg ataatattct
     1381 tattgggcgt tttcagtttc ttcatcgcga cgacgaataa tcaaccccgc cagccagaaa
     1441 ctaatcaccc acgtcaccag accgacggca taggtctgcc aaccttttgc ttcaaatccc
     1501 agtcccccca ccacgccatt aagaatgaat ataaggccaa tggcgaacgc atagtagtgc
     1561 cagtcacggc ggatttttac aggcagattc atggcagctc cgttaacgaa aaacaacatc
     1621 cttgcatatt ctgtaacaac cgtctgtggt gtagacaaca ttttcgtaat tgttaataaa
     1681 tttaacttaa ggttgccatt ttgtgactca caacgaagat tcacgcttcg ggaatattcc
     1741 caacatcaat ttaccatgat tctgatattg acaatctaaa tgacctgtca gacttgccat
     1801 aagccgctgg tatcacacca gaagagaagc agatgacttc cggaggtttt tatggctgat
     1861 ttcaccctgt caaaatcgct gtttagcgga aaatatcgca atgcctcttc aacgcctggc
     1921 aacattgcct atgcgttgtt tgtgctgttt tgcttttggg ctggggcgca attgctgaac
     1981 ctgttagtgc atgcgcccgg cgtctatgag cgtttaatgc aggtccagga aacaggtcgc
     2041 ccacgggtgg aaattggttt aggtgtcggc accattttcg ggctgatccc gtttttagca
     2101 ggctgcctca tttttgcagt ggtggcgcta tggctgcact ggcgacatcg ccgccagtaa
     2161 aatttacgtt gccagatgcg gcctgaaacg ccttatctgg cctgacattc ctgtttgatc
     2221 actgtttcat acaacgcgcc cgccgttcgt ctacgcgctt cgcaaaccag gccgtagtca
     2281 ggttgcgggt gattttcggg ctttccagtt gaatgccagg caacatttcg cggggtagtg
     2341 atttacccgt tttcgcttcg gcaagttgat aaactttctt gtatagcgcc gtctcctcaa
     2401 aagagaagct gtcacctttc tctaactgac ggcgaatttc gctgtcgctc atccccagtt
     2461 ttcccgccag tttgcgcgtt gccagttcgg ttttcccggg ttctttactg tcgtaacgaa
     2521 ttaaatcacc gtccagtgcc agcttcacgc cgctggcctt actgactgcg ttctgaaacg
     2581 cggcattacg gctggcgtac cagccagcgt taaaatcagc aaagcggtat atcggtgcgc
     2641 tatagctggc gggataattc agtaaatggt aagtaccaaa ccacaacccg ccgcggcgac
     2701 tgaagacttc ctgacgaact gtaccgtcca ttttccacgg ataccctttg gtatgctgtt
     2761 cggcaaaagc aatgctgacc tgcatcggcc caccggtgcg caccggatta agcgaaccaa
     2821 acagcgtctg ccccattggt accatgctga tcagatcgtc aaaaatcgcg ctcaattgct
     2881 tttctgtacg caccgaatct aaccgttcgc tataactttt gccgtttggc gatttgattt
     2941 ttagcgcggt atgaaccaga aaagcaggaa tgtgcatccg ttcagcacga cggtcaatct
     3001 cttgccaggc gatcttgctt aagcccggaa cggccggatc tgcctgataa tttgactcct
     3061 gctgcgccac cgccagcacc gagcagacgt tttccagcgt cggtgccagc ccctgacttt
     3121 caaaggtggt ggcgagatct ttcgcccagg catcgcggtc tttcacgctc gcaggcatct
     3181 tctgccgcac gacgctcgct acatctatcg ctttttcgcc ctttttcaac ggttgcggag
     3241 cctgactact acaccccgct aaaaccagaa ccgccaacaa tgacagcgaa gatagatgat
     3301 tgacgcgcga cattacttct cctttttagc tcaaggtatg ggttacttcc tgaattttgt
     3361 caccatccag ctcatgttca aagctgcgca gacgtttgta gatagacatc aactcaacca
     3421 gtgtggtcca tgagttaatc aggtactgga aagcaccgcg aacctgacca aaaacgttgg
     3481 taatctgcgt catcaggccg agcgtaatcg taccggcaac aattgacgga aacagcaaga
     3541 acaaaccgaa aacgttatcg acctgcaaat agagaatgcg ggcgatgttg aaatacatat
     3601 agtgaaaata gaggcgaaaa tagtttttcc gtacggcgct aaacagctcg cgtaccgtag
     3661 gcggcgtcgc gcgcgtggca tcgtcttcac cataaaccag ctctttacgg taggcggcct
     3721 ctactcgctg gtttttaaac tccagccccg gcagtttgat ccctaccact gccagcaatc
     3781 cggtccccat cagcgaccag acgatagcgg caatcaccag accatacgga atgtgcccga
     3841 taatcggcag ctccggcaca tgcgcggaga gcgttaccag caccggcagg aaggcgatca
     3901 acgtcatgat ggcgttgata aaactgacgc ccatattctc cagcgttgaa gcaaaacgca
     3961 tggtgtcttc ctgcacacgc tgtgcggcac cttcgatatg acgcagttgt tgccagttcg
     4021 ccatgtaata ttcgttcatc gccgtacgcc agcggaacac gtagtgactg acaaagaagt
     4081 tgttcagtac actgatcacc accgcgatta gcgcaatccc cagaaagacg cccacttcgc
     4141 ggtaaaattg ttcgatagtg actttatgcg gcgaacttag cgcggtttga atcagatcat
     4201 agaacggcgc ataccaggcg ttgacggcga ccccgacttc caccaaaaac caagtgacga
     4261 agataatcag cgcagtaccg agtattgacc agtattgcca acggtgcggg ctgtagataa
     4321 accagaaaaa tgcaaaaagt cctacgcaaa caatgtagta agcgtaaaaa atcaggaaat
     4381 ccaacgacca gaaacgcgcg gcgctaatcg ggatctgccc ggaagcgccg gtgatacgcg
     4441 ccacccagtc acccccaccg gcttgccaga agataacggc aatcaatgcc caaacaaagg
     4501 ccgagagaaa aaacgttccc ggctttggga aaaaagactt aaacatatgc actcctgcta
     4561 acttcttatc gttttaacaa ctgctgtgta ttaaccgcat gaccgctaca aagcgtcagc
     4621 caaatgaaac cgcccattgt gacccatagc aaggcgctta gttcgccagc ctgaccataa
     4681 gaattgtttc gacggatttc acaaaccaaa gattgtttac ataagcggat taacacgcgc
     4741 aggatcacaa atgcaggcat tggtgtgcag gaaaaagatc gtgtttacgc gccgttacgt
     4801 ttttaacact attattgcac taattcgccc tttgcaatcc atcaatgagt agtataaata
     4861 cgctcagtta ccttcattca atctatggac accaccgttg aaacgtagtc tgcttttttc
     4921 tgccgtgctg tgtgcggcgt cattgacctc cgtccatgcg gcgcagccga tcactgaacc
     4981 ggagtttgcc tctgatattg tcgatcgtta tgccgatcat attttttacg gcagcggtgc
     5041 cacggggatg gcgctggttg ttatcgacgg taaccagcgc gtctttcgta gttatggcga
     5101 aacgcgacct ggtaataacg ttcgcccgca gctggattcg gtcgtgcgta ttgcttccct
     5161 caccaaacta atgaccagtg aaatgctggt gaaattgctc gaccagggga ccgtaaagtt
     5221 aaacgatccg ctaagtaaat atgctccgcc aggcgcacgc gtgccgacct acaatgggac
     5281 gcccattacc ctagtgaatc tggcaaccca taccagcgcc ctgccccgtg aacagcccgg
     5341 tggcgcggca catcgtccgg tgtttgtctg gccaacgcgc gagcaacgct ggaaatacct
     5401 ttctacggcg aagctgaaag ccgcgccagg cagtcaggcg gcgtactcta accttgcgtt
     5461 cgacctgctg gcagatgcac tggcgaatgc gtccggcaag ccttataccc agttgtttga
     5521 agagcagatt acccgtccat tggggatgaa agacaccacc tacaccccct caccggatca
     5581 gtgccgccgt ttaatggttg ctgagcgcgg tgccagtccg tgcaataaca cgctggcggc
     5641 aattggcagc ggcggcgtct attccacgcc tggcgatatg atgcgctgga tgcagcagta
     5701 tttgtcatct gatttttatc agcgcagtaa ccaggcagat cgcatgcaga cgttgattta
     5761 tcagcgcgcg cagtttacga aagtgattgg catggatgtc cccggcaaag cggatgcgct
     5821 tggtttaggc tgggtatata tggccccgaa agagggtcgt ccggggatta ttcagaagac
     5881 aggcggtggt ggcggtttca ttacctatat ggcgatgatc ccgcagaaga atattggcgc
     5941 gtttgtggtg gtcactcgtt cgccgttgac gcgctttaaa aatatgagcg atggcatcaa
     6001 tgatctggtg accgagctaa gcgggaataa accgttggtt atccccgcat cctgattaat
     6061 actctgaagg caaacggttg atagcgacca gtttgccttt attcatttcg atatagccgc
     6121 ctttacgcaa agcagcgaga acttctgcga cgacagaacg cgatatgcgt gtacgttgat
     6181 ggatataatt catcacacca atacgtgatc gcaattcttc attccagtca atcattgata
     6241 ataatgttgc gcgggtttgt ttggctggta gatgatatcc cgtacaattt ccttctgata
     6301 ttaatttgta tggtatatcg tttttcatta aaccatcagc cagccccata atataaggag
     6361 cctgggtaat accgataagt acgttttctt ctctacgcag agagataact ccttctaaaa
     6421 taacgaaagt atcttcgtta ttgacatcac tggaaaatat aacttgcttt tcattattaa
     6481 actcgaagcg cgtaccgtat ctggacaaac atttatcgag cttaccaaat tcctgaagag
     6541 gtttaactac agataacatt tgcgcgtcct ttgcagtaat gcccgtcaaa tccttgacgg
     6601 gcattattta gattaaatta ccagtatttc ttcggagtga agaatattac caggtatatt
     6661 taacacccac gttcgcggac cagtcttgat ctacgtcacc accaccgagg tagttagcat
     6721 cggtataggc gctgaagttc ttggtgaagc tgaactgagt acccagacca acacgtaccg
     6781 cagacccttc agtaccgtta tcgatggaat caccgttcac atcgttatcg ttgttagagt
     6841 cgtcgtagac gtaagccagt ttgaagtacg gagtcagagc ctgatcttcg ctgtaggtga
     6901 aggtataacc tgcatctaca cccagttcat aacgcatgct gtcgtaagac tgaccgtcaa
     6961 ctttcatgtc gttgctcagc tggtagtcat
//
LOCUS       DBKEKI010000182         6967 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.184, whole genome shotgun sequence.
ACCESSION   DBKEKI010000182 DBKEKI010000000
VERSION     DBKEKI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.184"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..76)
                     /locus_tag="ACUQPX_003850"
     CDS             complement(<1..76)
                     /locus_tag="ACUQPX_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HHV9058366.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEM"
     gene            complement(73..990)
                     /locus_tag="ACUQPX_003851"
     CDS             complement(73..990)
                     /locus_tag="ACUQPX_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HHV9058367.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASCLMGSIAGIAELVKDSANKRTQP"
     gene            1136..1813
                     /gene="fetA"
                     /locus_tag="ACUQPX_003852"
                     /gene_synonym="ybbL"
     CDS             1136..1813
                     /gene="fetA"
                     /locus_tag="ACUQPX_003852"
                     /gene_synonym="ybbL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:1903988 - iron ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron efflux ABC transporter ATP-binding subunit
                     FetA"
                     /protein_id="HHV9058368.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            1800..2579
                     /gene="fetB"
                     /locus_tag="ACUQPX_003853"
     CDS             1800..2579
                     /gene="fetB"
                     /locus_tag="ACUQPX_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:1903988 - iron ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron efflux ABC transporter permease subunit
                     FetB"
                     /protein_id="HHV9058369.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVIQLKKK"
     gene            complement(2642..3496)
                     /gene="cnoX"
                     /locus_tag="ACUQPX_003854"
     CDS             complement(2642..3496)
                     /gene="cnoX"
                     /locus_tag="ACUQPX_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HHV9058370.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(3557..4366)
                     /gene="ybbO"
                     /locus_tag="ACUQPX_003855"
     CDS             complement(3557..4366)
                     /gene="ybbO"
                     /locus_tag="ACUQPX_003855"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HHV9058371.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(4356..4979)
                     /gene="tesA"
                     /locus_tag="ACUQPX_003856"
     CDS             complement(4356..4979)
                     /gene="tesA"
                     /locus_tag="ACUQPX_003856"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HHV9058372.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            4950..5636
                     /gene="ybbA"
                     /locus_tag="ACUQPX_003857"
     CDS             4950..5636
                     /gene="ybbA"
                     /locus_tag="ACUQPX_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HHV9058373.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            5633..>6967
                     /locus_tag="ACUQPX_003858"
     CDS             5633..>6967
                     /locus_tag="ACUQPX_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9058374.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSR"
BASE COUNT         1567 a         1800 c         1915 g         1685 t
ORIGIN      
        1 gcatctcggc tgccagcagc aacccgccga gactgagcca gaaaatatgt ggatgaacga
       61 ccattaactc catcatggct gagtccgttt gttggcgctg tctttcacca gctcggcaat
      121 cccggcaatc gaccccatca ggcagctggc ctctaatggc atcatcacta ctttgctgtt
      181 actggaggaa ccgatctgct gtaacgcttc ggtgtatttt tgcgctacga agtagttcac
      241 cgcctgaata tcaccggagg cgatagcctc agacaccatt ttggtggcgc gggcttctgc
      301 ttcggcggaa cgttcacgcg cttcagcctg taaaaacgcc gactgacgtt cgccttccgc
      361 tttcaggatt tgcgactgtt tttcaccttc ggctttgagg atttccgcct gacggatccc
      421 ttccgcttca agaatgtaag cgcgtttggt acgttccgct ttcatctgcg cgttcattga
      481 agagataagc tctgccggtg ggcgcacgtc gcgaatttca atacgggtga ctttaatccc
      541 ccacgggttg gtggcttcat cgacaatacg cagcaggcgt gagttgatgc tgtcgcgctg
      601 agagagcatt tcgtcaagtt ccattgaacc caacacggta cggatgttag tcatggtcag
      661 gttgatgatc gccagctcca gattgctgac ttcataggca gcgcgtggcg cgtcaatcac
      721 ctgaataaaa cagactgcgt cgatggtaac gttggcgtta tctttcgaga taacttcctg
      781 ggaagggata tcgagcactt gctccatcat attgatcttg cgaccaatgc gatccataaa
      841 cggcaccacc agactgagcc ccggctgtaa cgttttggta tagcgaccaa aacgttctac
      901 cgtccattga tagccctgcg gcacgatttt gacgcccgcg ccgacaatga ccagcgcgac
      961 aaaaatgaga atcgggataa agataagcat cggaaaaacc tcctgttgta ccgtccataa
     1021 tcagcaaaat tgctgcttga ttaaacaaat tatacctgat tactgaaaga gagttccccc
     1081 ttattcctgc gaaggataaa ctgtttttag taaaaatcag aaaaagggaa cagcgatgca
     1141 ggaaaatagt cctttgcttc agctacaaaa cgtaggatat ctggcgggtg atacgaagat
     1201 tcttaataac atcaattttt cgctgcgtgc tggcgaattt aagttaatta ccggtccttc
     1261 tggttgtggc aaaagtacgc tgctaaaaat agttgcttca ttgatcagcc caaccagcgg
     1321 aacgttactg tttgaaggtg aggatgtcag cacactaaaa ccagaaatct accgccagca
     1381 agtctcttac tgcgcccaga cgccgacgct gtttggcgat acggtatacg ataatctgat
     1441 ctttccctgg cagatccgtg accagcagcc tgacccggcc atttttctcg attttctcga
     1501 acgcttcgct ttgccggaca gcattttgac gaagaatatc gccgagctat ctggtggtga
     1561 aaaacaacgc atctcattga ttcgtaacct gcaatttatg ccgaaggttt tattgctgga
     1621 tgaaataacc agtgcgctgg atgaaagtaa taaacataac gtcaatgaga tgatccatcg
     1681 ttatgtgcgc gagcaaaata ttgccgtgct gtgggtgaca cacgataaag acgaaattaa
     1741 tcatgcggat aaagtgatta cactgcaacc gcatgccgga gaaatgcagg aagcacgcta
     1801 tgaactcgca taatattact aacgaatcat tagcactggc attaatgctg gtggtggtgg
     1861 caatcttaat tagccataaa gaaaaactgg cgctggagaa agatattctc tggagcgtcg
     1921 ggcgagcgat aattcagctg attattgtcg gctatgtgct gaagtatatt ttcagcgtgg
     1981 atgatgccag cctgacatta ttgatggtgt tatttatctg ctttaatgcg gcgtggaacg
     2041 cgcaaaaacg cagtaaatat attgctaaag cttttatctc atcgtttatt gccattacgg
     2101 tcggggcggg aattaccctg gcggtgctga ttctctcagg gtcgattgaa tttatcccga
     2161 tgcaggtgat ccctgtcgcc gggatgattg ccggtaacgc catggtagcg gtggggttgt
     2221 gttacaacaa tttagggcaa cgggtcatta gcgaacagca acagattcag gagaaactga
     2281 gtcttggcgc gacgccgaag caggcttcag cgatattgat tcgcgacagt attcgcgcgg
     2341 ctttaattcc gacggtcgat tcagcaaaaa cggttggctt agtgagttta ccaggaatga
     2401 tgtccgggct gatatttgcc gggattgatc cggtgaaggc gattaaatat cagattatgg
     2461 tgacctttat gctgctctca accgccagct tgtcgaccat tattgcctgc tatttaacct
     2521 atcgtaagtt ttataattcg cgccaccagt tggtggtgat tcaattgaag aagaaatgat
     2581 gcgatgccgg atgcgcagca tcgcatccgg cgttgtggtt catgtgccga acaacggaat
     2641 atcaatacaa caatgcatac agctggcggc gatacttcga cgccagtgca tcacccgtac
     2701 ccagcgcagc gaggatctcc tggaacgttt tacgcgtctg accgtctgcg gcggtgagat
     2761 ctttacgcag atgcccgaac agcaactcca gcgcctcttc attgcgccca acctgatgca
     2821 gttgcagcgc cagttgcgtc gccagtgcgg catcttctgg attctccgcc acctgctgtt
     2881 gcaactgttg aatttccggc ttatcagccg cctgcttcag cagttcgatt tgcgctacca
     2941 gcccctggta gcgggtgtcc tgatcctgca acggaatggt tttcagcacc gcttccgcat
     3001 cttcagaacg gttcagcgca atcagcgttt ctgccagcag caggccgatc tccccgttct
     3061 gattcgacaa ctgccaggcg tctttcagca atggcagggc atcggtgtaa ttgccttcct
     3121 gcatcagttg catcgcctgc tgcgctttca gctcttcttc gcgcggcagc actttatcca
     3181 gcagggcgcg gatcgcctct tccggttgcg gcccctggaa gccatctacc ggttgcccgt
     3241 tctggaacag atacacggtc ggaatcgcgc gcagaccaaa ctgggcggca atcatctgct
     3301 ccgcgtcgca gtccagcttc gccagaataa attgcccgtt gtactgcgcc gcgaggcttt
     3361 ccaggatcgg ggttaactgc aaacagtgct ggctacgttc agaccaaaaa tagaacagca
     3421 ccggagtggt catcgactgt tcaagaacct gttgcaggtt agattcgtta atgttgacaa
     3481 tattttctac ggacatggag tcgctctctg ttgtcgatat tttctttgac atgggggctt
     3541 aagcgcgcgc ttcaactcac ccctgcaata ttttgtccat cacgcgccca ggcagcaggc
     3601 gctcgagcac cattactgcc caggtcacca gcgttaccgg atagcgcatc ttcggcttct
     3661 cgctaataaa agcatggcgc actttgtcga ccaccgcttc cggtcccaac gtaaagcggg
     3721 cggcgatgcc gggattttcg actggtttat cactttgcgt ctggttgacg ttgtcggtga
     3781 agcgagtacg aatgggaccg ggttcgatca ggctgacttt aattccgctg tggcgtagct
     3841 ccatgcgcag cgcgtctgac cacgcctcca gcgcatattt actggccgcg taagcgccac
     3901 gacccggcgt ggagattaat cccatcaccg atgatgtcat cacaatacgc ccttcaccgt
     3961 gcggtaacat cgcgggtaac aggcgcatgg tgagctggtg tgcgccgaaa aagttggcgg
     4021 aaaactgctg ttccatctgc gcacggctga tggtggaaag ggggccatac atgccgaatc
     4081 cggcattgtt aaagatccca tacagacaat tatcggtcag ggcgatcacc tcgtctgctg
     4141 cgcgattaac actttctggt gaatccagat cgatcaacac gccggtaaat cccatgttgt
     4201 tcatacgctc aacatcattc ggttttcggc aacctgccag cacatgaaaa ccctggcgtt
     4261 ttaattcgag cgcgctttcc aggccaattc cactggaaca tccggtaatt aagaccgatt
     4321 tttgcataac tttacctgtc aggatctccg ttgctttatg agtcatgatt tactaaaggc
     4381 tgcaactgct tcgccatcca gtcggcaata aacggctggg cgtcgcggtt gggatgaata
     4441 ccgtcatcct gcatccattg tggcttgagg tagacctctt ccataaaaaa gggcagcagc
     4501 ggaacatcaa actctttggc gagtttgggg taaatggcgc taaaggcttc attataacgg
     4561 cgaccatagt ttgcaggcag acgtatttgc attaacaatg gttcagcgtt ggcggctttg
     4621 acatcctgca aaatctggcg cagcgtttgc tcggtttgct gtggctgaaa accacgcaaa
     4681 ccatcattgc cgcccagttc aaccagcacc caacgcggct gatgctgttt gagcagagcc
     4741 ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga tgctggcatt aactaccgac
     4801 gttttactct gccacttatc attcaacaag gcaggccagg ccgcgctggc agacattcga
     4861 tacccggcgc tcaggctatc acccagaatc aataacgtgt ccgctgcggc ggcacggaag
     4921 gttaacagga ccaggaacag gaagggcaaa tgccagcgga aaacattgtt gaagttcatc
     4981 atcttaagaa gtccgtcggt cagggggagc atgaactctc catcctcacc ggagttgagc
     5041 tggttgtcaa acgtggcgag accatcgcac tggtgggcga gtcgggatcg ggtaagtcaa
     5101 ccttgctggc gatcctcgcc gggcttgatg acggcagcag tggcgaagtg agtctggtgg
     5161 gacaaccgct acataatatg gacgaagaag cgcgggcaaa gttgcgcgcg aagcacgtcg
     5221 gctttgtttt tcagtcattt atgttaattc ctacccttaa cgcgctggaa aacgtcgagc
     5281 ttccggctct gctgcgcggt gagagtagcg cggaaagtcg taacggggcg aaagcgttgc
     5341 tcgaacagtt agggctgggt aaacgtctgg atcatcttcc ggcgcagctt tccggcggtg
     5401 aacagcaacg agtggcgctg gcacgagcct ttaatggtcg acctgatgtg ctgtttgccg
     5461 acgaacccac cggcaacctt gaccgccaga cgggcgataa aattgccgac ctgctgtttt
     5521 ccctcaaccg tgaacatggc accacgttga ttatggtgac ccacgacctg caactggcgg
     5581 cacgctgcga ccgctgctta cggctggtga acgggcagtt gcaggaggaa gcatgattgc
     5641 acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta attgtctggc tggcgctaag
     5701 cctggcggtg gcctgcgtgc tggcgctggg caatatcagc gatcgcatgg agaagggctt
     5761 aagccagcaa agccgtgagt ttatggcggg cgatcgggcg ttgcgcagtt cacgcgaagt
     5821 gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg aaagtcggca agcagctgac
     5881 tttcgccaca atgacctttg caggcgacac accgcagctg gcgaacgtca aagcggtgga
     5941 tgatatctac ccgatgtatg gcgatctgca aactaatccc cctggcctga aaccgcaggc
     6001 gggcagcgta ttgctggccc cacgcctgat ggcactgctt aacctgaaaa cgggcgacac
     6061 cattgacgtg ggcgatgcca ccttgcggat tgccggagaa gtgattcagg aaccggattc
     6121 cggttttaac cccttccaga tggctccgcg tctgatgatg aatctggcgg atgtcgataa
     6181 aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt tataaattcg gcggcaacga
     6241 gaaccagctc gacggctatg agaaatggtt gttacctcag cttaaacccg aacaacgctg
     6301 gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg atggaacgct cgcaacagtt
     6361 cctgctgctt tcggcgcttc tgaccttgct gctggcagtg gcagcggtgg cggtagcgat
     6421 gaatcattac tgtcgcagtc gctacgatct ggtggcgatc ctcaaaacgc tgggggcagg
     6481 gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg atggtgctga cgctttcagc
     6541 cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg ttgatggtgc tgctcaagcc
     6601 cgttctgcct gctgcactac cgccagccag cctctggccg tggctgtggg cgcttggcac
     6661 catgacggtc atctcgctgc tggtggggct acgaccatac cgcttgttgc tggcaacgca
     6721 gcctttacgc gtattacgta atgatgtggt cgcgaacgtc tggccgctga agttttatct
     6781 gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga ttaatgggtg gcagcatgct
     6841 gctttgggcg gtgctggcgg gcgcggtagt actggctttg ctgtgcggtg tgctgggctg
     6901 gatgctgctg aatgtacttc gccgcatgac gctgaaatcg ctgcctctgc gcctggcggt
     6961 tagccgc
//
LOCUS       DBKEKI010000183         6903 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.185, whole genome shotgun sequence.
ACCESSION   DBKEKI010000183 DBKEKI010000000
VERSION     DBKEKI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.185"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(225..1067)
                     /gene="purU"
                     /locus_tag="ACUQPX_003859"
     CDS             complement(225..1067)
                     /gene="purU"
                     /locus_tag="ACUQPX_003859"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HHV9058375.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1117..1575)
                     /locus_tag="ACUQPX_003860"
     CDS             complement(1117..1575)
                     /locus_tag="ACUQPX_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HHV9058376.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1688..2593
                     /gene="rssA"
                     /locus_tag="ACUQPX_003861"
     CDS             1688..2593
                     /gene="rssA"
                     /locus_tag="ACUQPX_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HHV9058377.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            2685..3698
                     /gene="rssB"
                     /locus_tag="ACUQPX_003862"
     CDS             2685..3698
                     /gene="rssB"
                     /locus_tag="ACUQPX_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HHV9058378.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            3900..4808
                     /gene="galU"
                     /locus_tag="ACUQPX_003863"
     CDS             3900..4808
                     /gene="galU"
                     /locus_tag="ACUQPX_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HHV9058379.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(4952..5365)
                     /gene="hns"
                     /locus_tag="ACUQPX_003864"
     CDS             complement(4952..5365)
                     /gene="hns"
                     /locus_tag="ACUQPX_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HHV9058380.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            5970..6587
                     /gene="tdk"
                     /locus_tag="ACUQPX_003865"
     CDS             5970..6587
                     /gene="tdk"
                     /locus_tag="ACUQPX_003865"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HHV9058381.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
BASE COUNT         1822 a         1507 c         1691 g         1883 t
ORIGIN      
        1 gaaccttcga agtcgatgac ggcagattta cagtctgctc cctttggccg ctcgggaacc
       61 ccaccacggg gtaatgcttt ttactggcct gctcccttat cgggaagcgg ggcgcatcat
      121 atcaaatgac gcgccgctgt aaagtgttac gttgagaaaa atgaactggt tgcgtaattt
      181 tcatccgtaa cggattaaag gtaaccagtt atttttgctg gcgattaaag aataatcgtc
      241 cgattaccgt aaacaaagac gcgttgcgcc agtactttgt atagcgcacg acttaagacg
      301 tttttctcga cgtcacgacc tgcgcgcatc atatcttcag ctgtgtaggt atgatcgaca
      361 tgaataacgt cctgcatgat gattgggcct tcgtccagat tgtcattcac atagtgagcg
      421 gttgcgccaa taatcttcac accacgttca taggcctggt gataaggacg tgcgccgata
      481 aacgcaggca ggaatgaatg gtgaatattg atgattttat tcgggaagcg tgccacaaat
      541 tccggcgtta atacccgcat atacttcgcc agcaccacat agtcaggttg ataagcatca
      601 atggcgtccg ccatcttttg atcgtgctcg ttgcggctta acccttcatg gcttaccagc
      661 tcaaacggaa tatcaaaacg ctcaaccaga gaacgtaaag tatcgtggtt accaataact
      721 gccgcgattt cgacatccag accgccgtaa ttggctttca tcaacaaatc gccaaggcaa
      781 tgcgcttctt tagtgaccag aatcactatc cggcgacgac cggcaggatt cagctcacgc
      841 acggagcctt ctggcaatgc gctatcgaga tccgccagca gggtggaatc attaaaaatc
      901 ccttccagtt ccgtgcgcat aaaaaagcgc ccggtacggt gatcaacaaa ttcattgttc
      961 tgtacgatat ttaactcgtg cttgtagcaa atattggtaa tacgtgcgat cagacctttt
     1021 tggtccggac aaatagtacg cagaacttta cgttggagtg aatgcattgc tggaaaaacc
     1081 ttgttgagag tgtttgctaa accgtaccgt caaccattat tggccgcagc actttttaaa
     1141 ttttttacct gaaccacaag ggcagggatc gttgcgacca aactgcggac gtgtaccgtc
     1201 aatatagtac cattgaccgt tttctttcaa aaatcgcgaa cgttcaatta ttgcacctgt
     1261 tttgccacct tcggtaaatc gagcaacaaa actgacaaat ccgatattgt cggcgtcctg
     1321 ccaacaatgt tcaaatacgg tcaatcccaa ccattcagta tgcgcaaatc cggtcatcaa
     1381 ttcggcacgt aacgccgctg ctccacaaga gggatgccag gtctttatta aataatcagc
     1441 gtcttgcatc acaaaagcgc agtaacgcga acgcatgaga tgttctggat caggtgcaac
     1501 cttttcacca gacacataag ggtggcaaca taggctatac tcgacagcac taccacaggg
     1561 acaaagctga gacacaaata atctccctgg aaacaataac ggcgtattaa ccgcctgagt
     1621 agcactatgt taaccgagca gtagcgatgt ggctacgatt gcattccagg ggaatcttgc
     1681 gggaataatg agaaagataa aaatagggct ggcgctggga tctggcgcgg cgagaggttg
     1741 gtcgcatatt ggcgttatta atgcgctaaa aaaagtgggt attgaaattg atatcgttgc
     1801 aggatgttca attggttcgc tggtgggcgc ggcctatgca tgcgatcgat tatctgcgct
     1861 ggaagattgg gtaacctctt tcagttattg ggacgtttta cgcctgatgg acctctcctg
     1921 gcagcgcggt gggttactgc gcggcgagcg tgtcttcaat caatatcgcg aaataatgcc
     1981 ggaaacagag atcgaaaatt gttcccgtcg ctttgcggct gttgccacca atttaagtac
     2041 ggggcgtgaa ttatggttta ctgaaggcga tctccatctt gctattcgcg catcatgcag
     2101 tattccagga ctcatggcac ccgttgcaca taacggctac tggctggttg atggggcagt
     2161 agttaaccca attcctattt ctcttacgcg tgcattgggg gctgatattg tgatagcggt
     2221 cgacctgcag cacgatgctc atttgatgca acaagatctg ctctccttta atgtcagtga
     2281 agaaaatagc gagaatggtg attctctacc gtggcatgcg cgtctgaaag aaaggttagg
     2341 cagcataacg acacgtcggg ctgtgacagc gccaacggca acagagatta tgaccacgtc
     2401 tatccaggtg ttggagaacc gccttaaaag gaaccgcatg gcaggtgatc cgcccgatat
     2461 tctgattcaa cctgtttgcc cgcaaatatc tacgcttgat ttccatcgcg cgcacgctgc
     2521 cattgcggcc ggacagctgg cagtggaaag gaaaatggac gaacttttgc cgttggtacg
     2581 caccaacatt tgaccagaat ttttatctac acttaagtta attctgacag gcgcaggtgg
     2641 caatagcatg ccactattga gtaaagccag tcaggggaga gaacatgacg cagccattgg
     2701 tcggaaaaca gattctcatt gttgaagatg agcaggtatt tcgctcgctt ctggattcat
     2761 ggttttcctc attgggagcg acaacggtac tggcggctga tggggtggat gcccttgagt
     2821 tgctgggagg tttcactcca gacctgatga tatgtgatat cgcgatgcca cgaatgaacg
     2881 ggcttaaact gctggagcat atacgtaaca gaggcgacca gaccccagtt ctggtgatat
     2941 ctgccactga aaatatggca gatattgcca aagcgttacg tctgggcgtt gaagatgttt
     3001 tgctgaaacc agttaaagat ctgaatcgct tgcgcgagat ggtttttgcc tgtctctatc
     3061 ccagcatgtt taattcgcgc gttgaggaag aggaaaggct ttttcgcgac tgggatgcaa
     3121 tggttgataa ccctgccgca gcggcgaaat tattacagga actacaaccg ccggttcagc
     3181 aggtgatttc ccattgccgg gttaattacc gtcaattggt tgccgcggac aaacccggcc
     3241 tggtgcttga tattgccgca ctttcggaaa acgatctggc attttattgc cttgatgtca
     3301 cccgagctgg acataatggc gtacttgctg ccttgttatt acgcgcattg tttaacggat
     3361 tattacagga acagcttgca caccaaaatc aacggttgcc agagttgggc gcgttattga
     3421 agcaggtaaa ccatttactt cgtcaggcca atctgccggg gcagtttccg ctattagttg
     3481 gctattatca tcgcgaactg aaaaatctca ttctggtttc tgcgggtctg aatgcgacgt
     3541 taaataccgg cgaacaccag gtgcaaatca gtaatggtgt tccgttaggc actttaggta
     3601 acgcttattt gaatcaattg agccagcgat gcgatgcctg gcaatgccaa atatggggaa
     3661 ccggtggtcg actgcgcttg atgttgtctg cagaatgagc aaacgataac gcgggctaaa
     3721 tttgcattac ctgctaatgt cggctggtgg tactatcgtc gccattcgta taagtaattg
     3781 tcttaattat gctatctcgc ctccttttca gaacttagcc ccttcggggt gctgatatac
     3841 tgggatgcga tacagaaata tgaacacgtt caaaacacga acagtccagg agaatttaaa
     3901 tggctgccat taatacgaaa gtcaaaaaag ccgttatccc cgttgcggga ttaggaacta
     3961 ggatgttgcc ggcgacgaaa gccatcccga aagagatgct gccacttgtc gataagccat
     4021 taattcaata cgtcgtgaat gaatgtattg cggctggcat tactgaaatt gtgctggtta
     4081 cacactcatc taaaaactct attgaaaacc actttgatac cagttttgaa ctggaagcaa
     4141 tgctggaaaa acgtgtaaaa cgtcaactgc ttgatgaagt gcagtctatt tgtccaccgc
     4201 acgtgactat tatgcaagtt cgtcagggtc tggcgaaagg cctgggacac gcggtattgt
     4261 gtgctcaccc ggtagtgggt gatgaaccgg tagctgttat tttgcctgat gttattctgg
     4321 atgaatatga atccgatttg tcacaggata acctagcaga gatgatccgc cgctttgatg
     4381 aaacgggtca tagccagatc atggttgaac cggttgctga tgtgaccgca tatggcgttg
     4441 tggattgcaa aggcgttgaa ttagcgccgg gtgaaagcgt accgatggtt ggtgtggtag
     4501 aaaaaccgaa agcggatgtt gcgccgtcta atctcgctat tgtgggtcgt tacgtactta
     4561 gcgcggatat ttggccgttg ctggcaaaaa cccctccggg agctggtgat gaaattcagc
     4621 tcaccgacgc aattgatatg ctgatcgaaa aagaaacggt ggaagcctat catatgaaag
     4681 ggaagagcca tgactgcggt aataaattag gttacatgca ggccttcgtt gaatacggta
     4741 ttcgtcataa cacccttggc acggaattta aagcctggct tgaagaagag atgggcatta
     4801 agaagtaaca tccgtatcgg tgttatccac gaaacggcgt tgagcaatcg acgccgtttt
     4861 tttatagctt attcttatta aattgtctta aaccggacaa taaaaaatcc cgccgctggc
     4921 gggattttaa gcaagtgcaa tctacaaaag attattgctt gatcaggaaa tcgtcgaggg
     4981 atttaccttg ctcatccatt gcttttttga ttacagctgg agtacggcct tggccagtcc
     5041 aggttttagt ttcgccgttt tcgtcaacgt agctatattt tgccggacgc tgagcacgtt
     5101 tagctttggt gccagattta acggcagcaa ggctattcag cagttcgttc gggtcaatac
     5161 cgtcagcgat cagcatttcg cgatattgct gcagcttacg agtgcgctct tcaacttcag
     5221 cagcagccgc gctttcttct tcgcgacgtt cgttaacaac aacttctaat ttttccagca
     5281 tttcttccag cgtttcaagt gtacattctc ttgcctgcgc acgaagagta cggatgttgt
     5341 tcagaatttt aagtgcttcg ctcattgtag taatctcaaa cttatattgg ggtggtttgt
     5401 tgaggtaata atagagcctt aaattcagtt gtgcaatagc caggaatgta aggaattcaa
     5461 aattgttctt tattttgtgc cgccaataaa tatcttttca taaaattagc cagaaaagac
     5521 gcggcatata gccctattta caccgatgat ttcgcagcac gtgaggttaa aacttcctga
     5581 ttcatgtcac attttatggg gagattatcg taggctgacg acctttcagt cttctgtatt
     5641 agttgtgttt acgagaattc cctattaagc gaatgatgaa aagtagaaca gtcgcaataa
     5701 gagcatggac ttagtattgc actatctctt ggaggtcaac agagggctat tacttgcgca
     5761 acaggttaaa gattgtgaat agttaccagc agtcatttac ccgcttataa caagcgaggc
     5821 agttgtaatg atagttcaga aggattatgc aaggcttcgt aagggagaac gcatataccc
     5881 acttctgtgc atactgttga gctgaaaaac tgacgaatta tgataaactc cagccaactt
     5941 tatttcatat cattgagggc ctgtggctga tggcacagct atatttctac tattccgcaa
     6001 tgaatgcggg taagtctaca gcattgttgc aatcttcata caattaccag gaacgcggca
     6061 tgcgcactgt cgtatatacg gcagaaattg atgatcgctt tggtgccggg aaagtcagtt
     6121 cgcgtatagg tttgtcatcg cctgcaaaat tatttaacca aaattcatca ttatttgatg
     6181 agattcgtgc ggaacatgaa cagcaggcaa ttcattgcgt actggttgat gaatgccagt
     6241 ttttaaccag acaacaagta tatgaattat cggaggttgt cgatcaactc gatatacccg
     6301 tactttgtta tggtttacgt accgattttc gaggtgaatt atttattggc agccaatact
     6361 tactggcatg gtccgacaaa ctggttgaat taaaaaccat ctgtttttgt ggccgtaaag
     6421 caagcatggt gctgcgtctt gatcaagcag gcagacctta taacgaaggt gagcaggtgg
     6481 taattggtgg taatgaacga tacgtttctg tatgccgtaa acactataaa gaggcgttac
     6541 aagtcggctc attaacggct attcaggaaa ggcatcgcca cgattaataa gaatttcttt
     6601 actgacaggg tgagcagggc acttttatcc tgtcagttcg ttttacgcac ttcttccggg
     6661 ctatataccc ttctcggcag ttttttaacg ccgctatacg cctcacaggg ctcttaagca
     6721 ccgacgttga cttgtgacct gtaaagtaca atatccctgt gtttaggcgt tatacatcgt
     6781 cgcaaatatg atgaaggcta atgctgtcgg gttgctttgg gtaaacaaaa aaacggcccc
     6841 agaaggggta atgccagtca gttaagcaac tgactggctc tttttcgggg ctgtggggta
     6901 ttt
//
LOCUS       DBKEKI010000184         6895 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.186, whole genome shotgun sequence.
ACCESSION   DBKEKI010000184 DBKEKI010000000
VERSION     DBKEKI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.186"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            82..816
                     /locus_tag="ACUQPX_003866"
                     /pseudo
     CDS             82..816
                     /locus_tag="ACUQPX_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1119..1889)
                     /locus_tag="ACUQPX_003867"
     CDS             complement(1119..1889)
                     /locus_tag="ACUQPX_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058382.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1934..2420)
                     /locus_tag="ACUQPX_003868"
                     /pseudo
     CDS             complement(1934..2420)
                     /locus_tag="ACUQPX_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2380..2601)
                     /locus_tag="ACUQPX_003869"
     CDS             complement(2380..2601)
                     /locus_tag="ACUQPX_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058383.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2602..3285)
                     /locus_tag="ACUQPX_003870"
     CDS             complement(2602..3285)
                     /locus_tag="ACUQPX_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HHV9058384.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SRAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3349..3660)
                     /locus_tag="ACUQPX_003871"
     CDS             complement(3349..3660)
                     /locus_tag="ACUQPX_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058385.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(3657..4559)
                     /locus_tag="ACUQPX_003872"
     CDS             complement(3657..4559)
                     /locus_tag="ACUQPX_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HHV9058386.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LKVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4999..5958
                     /locus_tag="ACUQPX_003873"
     CDS             4999..5958
                     /locus_tag="ACUQPX_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HHV9058387.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAIIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFISDELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQLALVKAMVHA"
     gene            5958..6353
                     /locus_tag="ACUQPX_003874"
     CDS             5958..6353
                     /locus_tag="ACUQPX_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HHV9058388.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6536..>6895
                     /locus_tag="ACUQPX_003875"
     CDS             6536..>6895
                     /locus_tag="ACUQPX_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HHV9058389.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLNA
                     LYCCAKEKIGLLSDNRDA"
BASE COUNT         1881 a         1789 c         1674 g         1551 t
ORIGIN      
        1 gtcgaaccaa gtgcatgaac tgctgccctg gaacttctcg tctgtaaaat aatccttacg
       61 ctacgtactt ctcggggcgc ttacgttgat gcagaagcca tatgtgaagc ggcttcacga
      121 ccatcaatgc gttttgtcca gccaagaact gagacccagc aggcaataag ggcattacac
      181 cgggtcagag aatcgctcat cagagataag gtgaaaacaa ccaaccagat ccacggtttc
      241 ctgctggaat ttggaatcag cctgccgaca ggggatgccg taataaaacg gttgtctctc
      301 gtattggctg aacacgaaat ccccgaatat ctgagccgcc ttttggtgag gttgcatacc
      361 cactaccttt accttgttga gcaaatagca gagctggaat cagaattaag tcagtcaatc
      421 aacgctgatg ataccgctca gcgcattatg acaattcctg gtgttgggcc gattactgcc
      481 agcttactat cgtcgcaact tggtgacggg aaacagtttt catgcagcag agattttgcg
      541 gcttccacag ggctcgttcc ccggcagtat agcactggag gaaaatcgac tctgttgggt
      601 atcagtaagc gaggtgataa aaatttacgc cggttactgg ttcagtgtgc ccgctcattc
      661 atgatgcagc ttgaacgcca gcatggaaaa cttgctgagt gggttcggga gcaactcaac
      721 aagaagcatt cgaatgtggt tgcctgcgct ctggcaaata aactggcccg aattgcctgg
      781 gcaataacta ctcagcaaaa agaatatcag gcctgatata tagatgctga caaaactcaa
      841 ttagcttatt gcaaacaata agatacaagt taccgtctgg ttttgcgaag attgattatt
      901 gatgacatga acggcaaatc gacctgatta aaaacctaac aggggaaaat ggctcactga
      961 agccgagggg atttttagga ttatcaggtg cggaactcat cgaggcgcgg gcatcccttg
     1021 cccacagaga cgccggatag atttaagcaa gcccaccatt aatcaaaaat cagtgttgca
     1081 aaaacggggg tgaccataga ttttccccgc catcgtcatc atttccggat ttttgccagg
     1141 gcatccgcca tgctgcggat ttcggcccgg gcttcccgga caacttcccg cagaacacca
     1201 cggtcaaacc accggcgggg cggttcatcc ggtatacgca gcacatcgca ggccagccca
     1261 ctggacagga tatgctcaat acaatcgctg accagataca tttccaccgc cgaaaaacgt
     1321 gcgccccggt tcaggatggc atcgagcact acgggcaggt tttcatcgtc ttcatcgagc
     1381 cagcaggggt gatcgctttc cttcacgacc agccgaaagc ggcgcgtctg gcgggtcagc
     1441 aacgtctgac cgcttgtggt cagcgccgcc cggatacgcc gcgcatcttc tgccccccgg
     1501 caactgacac tgcatactgc atcgcgcacc gcatcaagaa ctgccctgat ttcgtcttcg
     1561 gtcagccatt caccggcata cgggaaagac acctgcgttc cgtccgccgg aagtaccggc
     1621 agcgactgcc actgactgcc ggacagaaca agttccgctc cggttccggt cagtttctga
     1681 atggcggagg ccaggtgcgg cggcagcacg ccatcgtggc ggttaacgag taacgtcgcc
     1741 agccggggcg catccagccg cacgtaaatg ctgatccagc ccggatagtt cgggttacgt
     1801 tggtcaacat ccgtctcttc cggccagccc tgcgccagca gcaggttacg ggcgtgacga
     1861 caaatcagag cattcagcgt ttcattcata attcatgaac tccctgtatc gccgcccgca
     1921 ggcggcgata acatcagtcg ttttcgtgtg tgttgttttt cagcaggctg cgggcctgat
     1981 caaccatcgc ctgtcggcgg gtaacgtatt ccgggagggg gaaaccgtga tcccgggcag
     2041 cccattcacg tttccacagt gcctgttcag cgatctgagt cagggtctcc agcagttcag
     2101 cagggacggt cacctggcgg ttttcgcgca cgttgctgat aatctccatc gccgcggcgg
     2161 aagaaatacc ggactcaatc cgctagtctt ccgggatgtc ctccaggtgc gccaggacgg
     2221 tcctgatttc atgatcggac agggaaatat ccattccgtc agcgagggcc tgaatctctt
     2281 ccggggacca gataaccagc atcagcggcg tgtctcccga atactttgct gccagcgccc
     2341 ggcatagcgt ttcgcatgtt ccgtacatac actctcccct caatctttga agcaggaatc
     2401 cagttccaga atttcggtat atgcctccat caagaggcgg tttgcgcgaa acaacgtctg
     2461 acgcaccaga cgttccggca gggtgtcagc attaaagctg taccgccgct ccatctcatt
     2521 catggttgta cgcaggtcgc acatggcatt ggccacctcc gccacttcgg cgcgaagttt
     2581 ttcgtgtccg gcatagttca tttacgcagc ctccggcata aaccagtcat cattcgcggc
     2641 cccctgctgc atggcccgct gtacggcagc cagtcgttgc tgtccggcac ggtgatactg
     2701 ctcaagcagc tcgataccga tataccggcg tccggactgg agggcggcga cgcaggttga
     2761 accgctgcct gcaaacgggt ccagcactat cgcattcggg tgtgtgaagc tctcaatcag
     2821 cggttgcagg ctggtaacgg gcttttccgt cgggtgatgg cggttgcccg aatatttcca
     2881 gcccaacacg tccggcagcg ggttttgtgg cagacgtgga cggccttttg ccaggatgta
     2941 ggcgcattcg tggcgatagc ccacatatgc ggccctcgat gtgtagtttt tggtgaagac
     3001 caggtgacca acaacgctga atcccgcatt tttccaggca gacataaagc gatcgacgcg
     3061 gttccagcca tagaagctca ccatcagcgc gtcttttttg agtacgcggt acatttcatt
     3121 acaggccggt tgcagccatt catcagtttt atcaccagca atggtacgcc cctgacggtc
     3181 acgaaaaccg acgagatatg gcggatcggt gaggatgaaa tcaatggcgt tatcaggaat
     3241 acgggccata acatcgatgc agttaccgag gacaaaacgg gacataacag atccttctct
     3301 tacagagggc acccctgtgg tgcccgttcc ccgctgcctg tgggctgttc accgcgtccg
     3361 gtaacgggtc aggccgaccg tggaataccg cagccgtcgt ctttttgaca gcaaggttat
     3421 gccgcgaaag cggcttgaag ggcgttcatc agaaaagggc gacgggctgg cgtacactgt
     3481 caccgacgcc agccgggagc ggtaaaagcg cccgtcccgt gcccgccgga cgcgggacac
     3541 tgaccacagg cagcgtttat gccgcgccgc gtcagccggc gcagtaaaaa gaggagcacg
     3601 gcgacatcct ttgtcggaaa agaaacgggc ggttgcccgc ccgtctccgt catacttcat
     3661 ccaccataat gcgccacatt gtcgactatc cacgcaggtc cggtgacttc cggctgctca
     3721 tcgtcatctg tcggggaaga ccattcaagt tccccccaca gcacatcgac gagctctcca
     3781 cgctcataca actgccagcc acagaaatca cagccctgtt cggcatacca gtgctccagc
     3841 gtgcagctga aacggcggct taattctgca ataacgtcgc tttccggctg acaccacggc
     3901 gtgtcgaaat ccacctgaat gaagttttct ccctgactgc tgatattcag gtcatatcca
     3961 caaggccact tcacgccata ccgttccgta taccagtgat atgcagaagg aacaccgttc
     4021 agaacaccac cattaaagcc gttcacttca acatccaggc gggtcggcag aaccttcagc
     4081 aggtcaaacg gcatggtttt ttccggcact acgttgtcac acagacggtt ccaccacacc
     4141 gatacatcct cgttacccca tatgtcacac cagtcacctc tttttgcggt gaaatgcaca
     4201 gtgatggttt ccctggcatc atccggtaat ccttcccagc gacgctggcc agtaccgctc
     4261 tgtaaccaga gttcatgcag catcaggcag ttttgttcat ccagcaacac accgttctgc
     4321 aggtgtgtca gccagcgggt gaacgcgata ttttccgtcg atacagcacc acgtccggca
     4381 gccgtcactc cggggcactg ttcagagcgg atattttcag tgatctgcag aagtccggca
     4441 cttccggcca gaaacagctg aataccttca tttgtggcgc ggcgataaag cggtgtgact
     4501 gcaccgctgg ccagtcgttt aatctcagcg atctgtgccg gttcaccaga aaaatacata
     4561 cgattcgagc accagtttgg catgggaatt ctcctctcac atgctgcctc catcaggccg
     4621 tgccgcacct gtaaccgggc gaccgcatac gaatgcaggt gcggccccga agccgcagcg
     4681 cgtggaggag gaacgacgaa acgtgaccgg gtgaggaggc cggcgacagc gacacacttg
     4741 catcggatgc agcccggtta acgtgccggc acggcctggt taagtagtat ccgtctcagt
     4801 tcgtggcgca aaatgagaaa ccaataagaa ctcaaaatga accacaacaa gaacataaag
     4861 agactctaaa agagcaacac cgcttgacat agactcaaaa tgagtcacaa taagaaacaa
     4921 taaaaaactc aaaaagatgc accgacataa aggatgctca atttgagata aacacaaact
     4981 caaaacgagg caaaaagaat gctaaaagta tcatgcgatg atggttcaac taacgtaaaa
     5041 ctggcctggc ttgaagatgg cgaggtaaga accagtctgt caggaaacag ctttaaagaa
     5101 ggctggaacc cgggactgtt taatgccggc aaagtttaca attacgtcgt ggatgaaaaa
     5161 aaatacacct atgatttggg aagcactgct atcataggta caacacatgt cagttatcaa
     5221 tacagcacca ccaatctgct tgccatacat catgcactgc tgacatccgg ccttcagcca
     5281 caggatgtag aactgacagt aacattacct gtgaccgaat tctttgataa tgacaatcaa
     5341 ccaaatgaag aaagaataga aagaaaaaag gcaaatgttc tccgtgagat atctctgaat
     5401 aaaggggaaa catttaaaat taaaaaagtt aatgtaatgc ctgaatcttt gccagctgca
     5461 tttgaatcac taaaaaaaga taaagtaaac aaacttgaac gcagcctgat tatcgatctt
     5521 ggaggaacca ctctggattg tggtttaatt ctcggtgcat ttgaaggcat ttctgaaatc
     5581 cgtggctatt cagaaatcgg gacatcccgc atcactcata ccgtcatgaa tgcgctgaca
     5641 aaagcctcca caccatgtaa ttattttatt tctgatgagt taataaaaaa cagacacgat
     5701 aatgaatatc tccagacatt aataaatgat gtagctgaaa taaaaaacat ttctcatgta
     5761 atagacagag aagtgaaatc tctggcagaa agcatcaggc aggaaatttc aacattctca
     5821 ggaatgaacc gaatctatct cactggcgga ggagccgagc ttatttaccc tcacataaag
     5881 caatatttcc cgaatctgaa agtgaacaag gtggatgaac ctcagttggc attggtgaaa
     5941 gcaatggtgc atgcgtaatg gaaagcagtg atccaaaaaa aagaaagaaa gttgtggcat
     6001 acctgcaccc ggcactttat ccgcaggaca acctgacaca acaaacaata gactctctcc
     6061 cagtacaaat gcggggagat ttctacagac aatcccttat ttgcggagca gctctttatt
     6121 ctgttgaccc ccgactgtta acgctaatca gcggtttttt cagtgagaaa atcactgcag
     6181 aaaatctggt gaaacttata gaacaaacaa caggatacac atccacatca attgatataa
     6241 gtgtgttaaa aaacatcata gaggcatctt cagaaaacaa atcagagagc atcacttcaa
     6301 aagatgattt cgaagaacaa accagacgca atctttctat gttaaaaaaa taataaaaga
     6361 agagcaggtt cagtcaatta cctgctcttc ttttatacat actaaaaaat cagattttta
     6421 cgaatattca gattatcata aaaaatgcaa cagacaggaa tgcaataatt ttgcattatc
     6481 tcgttgcttg taatgcaaat atgcattatt gtcagtgtgt gacaataaca gagagatgac
     6541 gatggcgacc attaacgcac gtattgatga tgatataaag aaccaagcag atgaagtgct
     6601 gaagctgatg aatatcagtc aaactcaggc tattgcagct ttttatcagt atattactga
     6661 acaaaagaaa ctgccatttg tgatcacctc aattgtaaaa acgccgcatg atttattacg
     6721 tgaaagtaca gacatgttag ctgaagctct tgcagtgatt tccaatctac aggtgtggac
     6781 tgaacagcag gatgggattg gaaaagcaaa gctaatggag tattaccgcc gcctcaacgc
     6841 actgtactgt tgtgcaaaag agaaaatagg tcttctttca gataaccggg atgct
//
LOCUS       DBKEKI010000185         6734 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.187, whole genome shotgun sequence.
ACCESSION   DBKEKI010000185 DBKEKI010000000
VERSION     DBKEKI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.187"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(112..1567)
                     /locus_tag="ACUQPX_003876"
                     /pseudo
     CDS             complement(112..1567)
                     /locus_tag="ACUQPX_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
     gene            complement(1564..2592)
                     /locus_tag="ACUQPX_003877"
     CDS             complement(1564..2592)
                     /locus_tag="ACUQPX_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HHV9058390.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            2689..3354
                     /locus_tag="ACUQPX_003878"
                     /pseudo
     CDS             2689..3354
                     /locus_tag="ACUQPX_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
     gene            3365..3880
                     /gene="ogt"
                     /locus_tag="ACUQPX_003879"
     CDS             3365..3880
                     /gene="ogt"
                     /locus_tag="ACUQPX_003879"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HHV9058391.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            4075..4827
                     /gene="fnr"
                     /locus_tag="ACUQPX_003880"
     CDS             4075..4827
                     /gene="fnr"
                     /locus_tag="ACUQPX_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HHV9058392.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            4979..5929
                     /gene="uspE"
                     /locus_tag="ACUQPX_003881"
     CDS             4979..5929
                     /gene="uspE"
                     /locus_tag="ACUQPX_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HHV9058393.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(5979..6236)
                     /locus_tag="ACUQPX_003882"
                     /pseudo
     CDS             complement(5979..6236)
                     /locus_tag="ACUQPX_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            6480..6734
                     /locus_tag="ACUQPX_003883"
                     /pseudo
     CDS             6480..6734
                     /locus_tag="ACUQPX_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
BASE COUNT         1929 a         1605 c         1459 g         1741 t
ORIGIN      
        1 tgttagcagt atcgaacaat ttagcccgag tttaattcag cacaggctcc gatcaggatg
       61 atttattcat agcaggaacc agcaaaaatg cgataaataa cagaccactg attagcagaa
      121 aggtatccga aaatgccata gtttgcgcct ccctcagcat caattgactc aataatttgg
      181 ttgaagcaag tatttccgat gtctggtcac ttctactgcg attaaaaaat aaaacacttc
      241 tggaaatgaa gtcattcacc gtatggggaa cagaaaccat tttttcaccc attctcgaaa
      301 aatgaaaatt agttcgatta ttgagaatag agccacacag ggcaatacca atcgcgcccc
      361 caagattacg tgttaaatta aatacaccag aggctaactt cagccgttct tttggaattc
      421 cccccagcgt taatgttacg atgggggcca tcgcaaactg ctgcgaaatc cctctaattg
      481 cctgtggaaa cagtaattcc tgccatcccc attcgtgggt tataggggta aaaagataca
      541 ttgaaaacac aaatccgccc agccccgcca tcaacagcca tcgcagattt atttttttac
      601 ttaaccagaa gtaaaaggga acagaaaata actggaaaat acctgttgtg caaacggaaa
      661 aacctatctc ttcggcattt aatccgcgaa tctgccccaa aaacacaggt attaaatata
      721 cagtagagaa tatcccaacc cctccagaaa aagaaaaaaa acatcccaga gtgaaatttt
      781 tatccttaaa agcatgaaga cccattatcg gattggatat tgtaagtgta cgcgcagcaa
      841 ataaaataaa actcaccaat gccagaactg atgtcagcaa aatagtatta tcatccagcc
      901 atccccagcg cgctccttct tcaagggtat attccagaca tcccagagtc atggccaaca
      961 ggataatact cgaataatca gcaactttca gtaaagagag atcaggttta tcaaaattaa
     1021 ccagaaaagg aatactcaaa actaaatata tgccaggtaa tatattgata taaaatagcc
     1081 atcgccagtc gaggttatca gtgagccagc cccccagagt tggccctagc gtcggtgaca
     1141 gtgaggccag cgcactgact accgcagcag ccaaccccaa ctctttaccc tggtaataaa
     1201 taaacgccgt cgtgaaaact aaagggatca tggatgcgcc agcagctccc tgtaaagcac
     1261 gaaacaaaat catgatctga atattccatg caagtccaca cgcgatactc attaaagtaa
     1321 atattcccgc ggatagagta aaaagccaac gagtggaaaa tacccgtgac agccacccag
     1381 agagcggaat aacaataatc tcagcaatta aatagctggt ctgaagccag gccatttcgt
     1441 ctttacctgc ggacagacca ccacctattt catttaggga tgacgataca atttgaatat
     1501 caagctgagc aataaataat cccatacaca tactggcgaa agcaatatgt ttacgcatag
     1561 acatcatctc tcatcaacac tgacggtgac ggacagtccc gggcgaagaa gttgctttaa
     1621 ttcctttgcg tcgtcaaatg caatccgtac cgggacacgc tgaacaattt tggtgaaatt
     1681 gccagtggca ttttctatcg gaatcaagct gaaactggct cctgtcgctg gggaaaggct
     1741 ttcaatgtga ccgtgaaaca cttccccttt gagaatatca gcacggatct cagccttcat
     1801 tcctggcttc attcccgcta tttggttttc tttataattt gcatcaaccc acaactcact
     1861 gactggtact agtgaaacta acgatgtgcc gccttccacc caactgcctg tatgggcgct
     1921 gcggtttgcg acaatgccat caatcggcgc acgaacaagg gtatattcca gatccagtag
     1981 tgcctgagtc aaagcggcct gaacttcttc gatactcgca cgtacgtttt cttcctgagc
     2041 agataaaact gccagccgtt gcttctccac caggaaatcg ttcgcggctt tgcgctcctt
     2101 tgctgcgacc tgctggtagt caaacctggc gttatcaata atttgttgcg atattgccgc
     2161 tgattgtgcg agtttttcat aacgttcagt atctcgcagg cgcttttgct cctcatgttt
     2221 tactgcctgc catgtttcac tggcactttg aattatcgac tgctgcatcg taatagtggc
     2281 ttgaatgcct tcgagattgg ctttgctact ttttattttt gcctcaagcc tggcgacatt
     2341 tgcacgataa tcccgatcat cgagacgcag gataatatcg ccttttttta cgctctgatt
     2401 atcgcgcacc tctatagcag aaatataacc agaaacttta cttgccaccg tggtgatatt
     2461 accaccaatg taagcatcat ccgtcgtctg tataaatctt cctttgctta ccctccagat
     2521 aaaaaagaca acgccagtca aaagaatacc gatcgctacc accccaataa gctgtttttt
     2581 agaaattatc atcactcttt acaccgtata tatccggaaa cttgcatacg tcatatttaa
     2641 aatttaacat ggcttatttg atgatttctg acaaaatacc tactgtaaat gacaattaca
     2701 ctgcaaccca aagcatcacc agggcatcac attatcggac tggattcgga acatctgaat
     2761 ggaggtacag ttccctggca taaacatctc tatgcccagt tgctttaccc ggctgaagga
     2821 gtcgttcgtg tatgggctgg agaaagcgtc tggctagtcc atgccagcag cgcgctttgg
     2881 ctgcccccac aaatgccgca taagtttgtt gcgacaggca acgtactttt aaaaaccgta
     2941 ctggtttcag aagcggaaag tgaaacgctg ggaacagttt gcttcatgac gggtatttca
     3001 cccctgttaa gggaattact aattgcaatt aaccaacttc caccgtcgca aagtaccaca
     3061 gataaacaac agttacgttt ttcagccctc gagacactga tacttcagga aataaaaatg
     3121 ggggtgaaaa tgtcacttga actcccttgg ccaaatgatg agcggctaca gcagctttgt
     3181 gagaatttgc taaacaacca agggtatctg ccaacactgg ataatctagc ggataaaatt
     3241 aacgtcagta gccgtacact catgcgctta tttgttaaag agacgggatt aacatttcgc
     3301 cactgggttc agcaaatgca cgttatttct gcagtgacgc ttcttgatga tggagagaga
     3361 acggatgctg agattacttg aagaaaaaat tgccacgcca ctgggtccac tgtgggtgat
     3421 ttgcgatgag caatttcgcc tgcgggcggt tgaatgggaa gagtacagcg aacgcatggt
     3481 gcagctgctg gacatccatt atcgcaaaga aggctatgag cgcatttctg ccaccaaccc
     3541 aggtggttta agcgacaagc ttcgtgaata ttttgccggt aatcttagca ttattgatac
     3601 gcttcccact gccacggggg ggacgccatt tcagcgcgaa gtctggaaaa cactacgcac
     3661 tatcccctgc gggcaggtaa tgcattacgg ccaactggct gaacaattgg gccgccctgg
     3721 cgcggcgcgt gccgttggtg cggcaaacgg atcgaatccc atcagcatcg tcgtaccttg
     3781 ccatcgggtt attggccgaa acggcaccat gaccggatat gcaggcggag ttcagcgaaa
     3841 agagtggtta ttgcgccacg aaggttatct tttgctgtaa acattaaaca atttgtgcca
     3901 gcttgttcac acttttatgt aaagttaccc ttgacaactt aaggagtttc aaatagatag
     3961 acatatattt acatctaata tcggaattct ctgctgttaa ggtttgctta gacttacttg
     4021 ctccctaaaa agatgttaaa attgacaaat atcaattacg gcttgagcag acctatgatc
     4081 ccggaaaagc gaattatacg gcgcattcag tctggcggtt gtgctatcca ttgccaggat
     4141 tgcagcatca gccagctttg catcccgttc acactcaacg aacatgagct tgatcagctt
     4201 gataatatca ttgagcggaa gaagcctatt cagaaaggcc agacgctgtt taaggctggt
     4261 gatgaactta aatcgcttta tgccatccgc tccggtacga ttaaaagtta tacaatcact
     4321 gagcaaggcg acgagcaaat cactggtttc catttagcag gcgacctagt gggatttgac
     4381 gccatcggca gcggccatca cccgagcttc gctcaggcgc tggaaacctc gatggtatgt
     4441 gaaattccgt tcgaaacgct ggacgatttg tccggtaaaa tgccgaatct gcgtcagcag
     4501 atgatgcgtc tgatgagcgg tgaaatcaaa ggcgatcagg acatgatctt gctgttgtcg
     4561 aagaaaaatg ccgaggaacg tctggctgca ttcatctaca acctgtcccg tcgttttgcc
     4621 caacgcggct tctcccctcg tgaattccgc ctgacgatga ctcgtggcga tattggtaac
     4681 tatctgggcc tgacggtaga aaccatcagc cgtctgctgg ggcgcttcca gaaaagcggt
     4741 atgctggcag tcaaaggtaa atacatcacc atcgaaaata acgatgcgct ggcccagctt
     4801 gctggtcata cgcgtaacgt tgcctgattt ttccgcataa ctcactatcc ttctgtcata
     4861 tcattaaatt tttctgattt attgatctgg cagaaggttc atcactgttt cattcaccag
     4921 atatgggtta atcttttaat tacaaactgc gttgacagtt gttgtaagga gaccctgtat
     4981 ggctatgtat cagaacatgc tcgttgttat cgatcctaac caggacgacc aaccagcatt
     5041 gcggcgagct gtttatttac atcaacggat tggtggcaaa attaaagcct ttttgccgat
     5101 ctatgacttc tcatacgaaa tgaccaccct gctctccccg gacgaacgta ccgctatgcg
     5161 tcagggcgtc atcagccagc gtacagcctg gatccacgag caggcaaaat attatctcaa
     5221 tgctggcgtt cccattgaaa ttaaagtggt ctggcataac cgtcctttcg aagccatcat
     5281 tcaggaagtg atcagcggcg gacacgattt ggtgctaaaa atggcgcacc aacatgaccg
     5341 tctggaagcg gtgattttta cgccaacgga ctggcatctg ttacgcaaat gcccaagccc
     5401 ggtgtggatg gtgaaagacc agccgtggcc ggaaggaggc aaggcgctgg tggcggtgaa
     5461 tctcgccagt gaagagccgt atcataatgc gctcaatgaa aaactggtca aagagacgat
     5521 cgaactggca gaacaagtca accataccga agttcatctg gttggcgctt atccggtaac
     5581 gccaattaat atcgcgattg aactgccgga atttgacccg agcgtttata acgatgccat
     5641 tcgtgggcaa catttgctgg caatgaaagc cctgcggcag aaattcggca ttaatgaaaa
     5701 catgacgcac gtagaaaaag gtctgcccga agaggtgatt cctgatttgg cggagcattt
     5761 acaggcggga attgtggttc tgggcacggt cggacgcacc ggtatttcag cagcattcct
     5821 cggcaacacg gcggaacagg tgattgatca tcttcgctgc gacctgctgg tcattaaacc
     5881 tgaccagtat cagacacccg ttgaactgga tgacgaagaa gacgattaac gactcttcaa
     5941 taactgacaa ggcccgagtt atcgggccta ttttttactt actgctcttc gccgccaagg
     6001 aaataaattc ccaacgggat tgccagcaac acagtaaaga ccaggctata aacaaaaatc
     6061 atcgatgact aaatcacata cattgatgtc gtccactcag acagcggaat attgtactgt
     6121 tctataacgc cgccgatggt tgcgcgagtc acaaccgacg ccgcaataat gaaaaccgcc
     6181 aacaaaccaa agagaaattt cttccctttc gctgacttca gtttcgccat aatcatgatg
     6241 taatccttgt gcagacaaat ttcttgttac ctaaacgtaa caataccatg acgtttcacc
     6301 gttgtctgct ctgaaaacct cacctcttag ctggatttac aatcaacgca ttgattagtt
     6361 agctaatgtt gctacttcct tccctgacta ttgatgatct gaatgaattg tcgcggtaaa
     6421 atagccctgc caggggtttc cggcatgacg acaattatct ggaggagcct gataaaacaa
     6481 tgatcgctga actgtttaca aataatgcgc ttaatctggt cattattttc ggtagctgcg
     6541 cagcattgat tctgatgagc ttttggtttc gccgtggaaa tcgtaaaaga aaaggatttt
     6601 tattccatgc ggtgcaattt ttaatctaaa ccataattat cagtgctgtt ggtagcatca
     6661 ttaattatgt tatagaaaac tataaactca aatttatcac cccaggcgtt atcgatttta
     6721 tctgtacgtc cctg
//
LOCUS       DBKEKI010000186         6712 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.188, whole genome shotgun sequence.
ACCESSION   DBKEKI010000186 DBKEKI010000000
VERSION     DBKEKI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.188"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..106
                     /locus_tag="ACUQPX_003884"
     CDS             <1..106
                     /locus_tag="ACUQPX_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058394.1"
                     /translation="SREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            331..1986
                     /gene="opgD"
                     /locus_tag="ACUQPX_003885"
                     /gene_synonym="mdoD"
     CDS             331..1986
                     /gene="opgD"
                     /locus_tag="ACUQPX_003885"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HHV9058395.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            2126..2350
                     /locus_tag="ACUQPX_003886"
     CDS             2126..2350
                     /locus_tag="ACUQPX_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HHV9058396.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            2413..2949
                     /gene="rimL"
                     /locus_tag="ACUQPX_003887"
                     /pseudo
     CDS             2413..2949
                     /gene="rimL"
                     /locus_tag="ACUQPX_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
     gene            complement(2944..3924)
                     /gene="ydcK"
                     /locus_tag="ACUQPX_003888"
     CDS             complement(2944..3924)
                     /gene="ydcK"
                     /locus_tag="ACUQPX_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HHV9058397.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWLDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            4048..5040
                     /gene="tehA"
                     /locus_tag="ACUQPX_003889"
     CDS             4048..5040
                     /gene="tehA"
                     /locus_tag="ACUQPX_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HHV9058398.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            5037..5630
                     /gene="tehB"
                     /locus_tag="ACUQPX_003890"
     CDS             5037..5630
                     /gene="tehB"
                     /locus_tag="ACUQPX_003890"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HHV9058399.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            5933..6601
                     /locus_tag="ACUQPX_003891"
     CDS             5933..6601
                     /locus_tag="ACUQPX_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HHV9058400.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(6619..>6712)
                     /locus_tag="ACUQPX_003892"
     CDS             complement(6619..>6712)
                     /locus_tag="ACUQPX_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HHV9058401.1"
                     /translation="QQTPGQVENRALYPRPEGWGFTAHRIKSAI"
BASE COUNT         1719 a         1556 c         1641 g         1796 t
ORIGIN      
        1 cagtcgcgaa gcatttgagc aggcgttggg ttgtccggtt ttggatgagt tccagcttta
       61 ccaggaagcg gaagaacgca gtaaacgtcg ctgtggtttg ctttaaattc tgaccatccg
      121 cctttgcaaa aatttgcctg attttacaaa cgaatcaggc tcatcccatc gacataaaaa
      181 aaatgccgat ttatgcatat tctctcagtt caacaattgg attattaata aatattgtct
      241 agagtgagcg gtcataaata agcactttct tgccgctgaa aacgaccagc gcgggaccat
      301 tcacaacacc agaaggactc actttcaggt atggatcgta gacgatttat taaaggttca
      361 atggctatgg ccgccgtgtg cggtaccagc ggcattgctt ctcttttttc tcaggcggca
      421 ttcgcggcag attctgatat tgccgacggg caaacccagc gttttgactt ctccattcta
      481 cagtcaatgg cgcacgactt agcgcaaaca gcgtggcgtg gtgcgcctcg tccgttacct
      541 gacacgctgg cgacaatgac gccgcaggct tataacagta ttcaatacga cgccgaaaaa
      601 tcgctctggc ataacgttga gaaccgtcaa ctggacgctc agttcttcca tatgggaatg
      661 ggattccgtc gccgcgttcg tatgttttct gtagatccag caacacatct ggcgcgtgaa
      721 attcactttc gcccggagtt gttcaaatac aacgatgcag gtgttgatac caaacaatta
      781 gaagggcaaa gcgatctcgg ctttgccggt tttcgcgtgt ttaaagcccc cgaactggcg
      841 cgccgtgatg tagtatcatt tctcggcgcg agttatttcc gcgccgttga tgatacatat
      901 caatacggtt tgtcggcccg cggcctggcg atcgacactt acaccgacag taaagaagag
      961 ttccccgact ttaccgcctt ctggtttgat acggtaaaac cgggggcaac tacctttacc
     1021 gtttatgcgt tgctcgatag cgccagcatt actggtgcct ataagttcac tatccattgt
     1081 gagaaaagtc aggtgattat ggatgtggaa aatcacctgt atgcgcgcaa agacattaaa
     1141 cagctgggca ttgcgccgat gaccagtatg ttcagctgcg gtactaatga acgtcggatg
     1201 tgcgatacaa ttcatccgca aattcatgac tctgatcgtc tgtccatgtg gcggggcaat
     1261 ggcgagtgga tttgccgtcc gctgaataat ccgcaaaaat tgcagttcaa tgcttacacc
     1321 gacaacaacc cgaaagggtt tggtttattg caactggatc gtgacttctc ccattatcag
     1381 gacattatgg gctggtataa caaacgccca agtctgtggg tggaaccgcg taacaagtgg
     1441 ggtaagggca ccatcggcct gatggaaatc ccaacaacgg gcgaaacgct ggataacatt
     1501 gtctgcttct ggcagccaga aaaagctgta aaagcaggtg atgagtttgc attccagtat
     1561 cgtctgtact ggagtgcgca accgcctgtt cattgcccat tagcgcgcgt tatggcgacg
     1621 cgtaccggca tgggcggttt cccggaaggt tgggcgccag gtgaacacta tcccgaaaaa
     1681 tgggcgcgtc gttttgccgt cgatttcgtt ggtggtgatc tgaaagctgc cgcgccaaaa
     1741 ggcattgagc cggtgattac gctttccagt ggggaagcga agcaaatcga aattctctat
     1801 attgaaccca tcgatggtta tcgtattcag tttgactggt atccgacttc ggactccact
     1861 gatccggtcg atatgcggat gtatctacgt tgtcaggggg acgctatcag tgaaacatgg
     1921 ctgtatcagt atttcccgcc agcgccggat aaacgtcagt atgttgacga ccgcgtgatg
     1981 agttaatcgt tttttcttcg gcaccttctt cgggaggtgc cgtctggtta aacacgatcc
     2041 cgctcgcatt tttccctaag ttaaatgagt aatctgatgg tgtgtatttc agatacaccg
     2101 tgtcagccac taacagggag tgcgtatgtt tccagaatac cgagatttaa tatcccgtct
     2161 gaaaaacgaa aatcctcgct ttatgtcctt gttcgataaa cacaataaac ttgatcatga
     2221 aattgccaga aaggaaggtt ccgacggtcg agggtacaat gcggaagtgg tccgcatgaa
     2281 aaaacaaaag ctacagttaa aagatgagat gctcaaaatc ctgcagcagg agagcgtcaa
     2341 agaggtgtaa actttcctaa gccgccagcc aggcggcttt tttaacaaca gcatggattg
     2401 actggagata agatgactga aacgataaaa gtaagcgaat cacttgaatt acatgctgtt
     2461 gcggaaagtc acgtcacacc tctttatcag ttaatctgta aaaataaaac ctagttacag
     2521 cagtcgctaa actggccgca gttcgttcag agtgaagagg acacgcgaaa aacggtgcag
     2581 ggtaatgtga tgttgcatca acgcggctat gccaaaatgt tcatgatttt caaagaagat
     2641 gaacttatcg gcgttatctc gtttaatcgt attgaaccac tgaataaaac tgctgaaatc
     2701 ggctactggc tggacgaatc tcatcagggg caggggatca tttctcaggc gctgcaggca
     2761 ttgattcatc attacgctca gtctggtgaa cttagacgct tcgtgatcaa atgtcgggtg
     2821 gacaatccgc aaagcaacca ggtcgctttg cgcaatggtt ttatccttga aggttgcctg
     2881 aaacaggctg agttcctgaa tgatgcctat gatgatgtga acttatacgc gcgtattatc
     2941 gattcataat aaccctgcca gcggcgttcg cgtaatgcgc tcctcgccgt taatgacttt
     3001 tggaccacgg acatgaaccg tgtcgccatc aaaggcttca acgacggcgt ggtcggttaa
     3061 ctccacatgg ttttcaatta tcaccgcacc tgtaatacgg ctctcacctt gtattacaac
     3121 gtgctcgtcg agcagaatcg gtccaccacg taccacggca tttccgccaa tcaagacatg
     3181 atgttttaac acacaattac cttccacaat ggcatattcc gccacctgcg aactgtaatg
     3241 aatcgtcgga atggcatctt cttctatgcc agctttcacc tgcgcgtgac cgtagacttt
     3301 agcgcaatcg catagccaga cgttgttctc ttcattacct tcaacactgg caaaatcaaa
     3361 aacttcggcg cgatgttcaa taaaagcata ccggacgacg gcatcgccat aaatttgtgc
     3421 ctggtggaca atacgcgagg cgctaaccct tgcgcgatca taaatttgga gcaggagttg
     3481 atggtcgggc gttaagcctt gtgcggcgac gatcatagaa tgttgatcaa ttaaggcgtg
     3541 accaaatatt ctacattgcc cgtaaactaa tgagtcatga atggtgacac tgtcacttat
     3601 atatgcaccc tggctgattt cactattatc gatccagacg ttatccgttg cgtaaacctc
     3661 tccccacagc acgctggtgc cggtgatacg cgtattaccg gaaatcactg ttccgccgaa
     3721 cgcaatagca ttctggtcgt aaatccagca atttccttcc tgtgctaaca ccgtttcgcg
     3781 atcgagccag ccgccagcgg ttcctgctat cacatcgtta aaatcgctaa tggcgataat
     3841 ctgccgtaat aacacacttt ttttagtgcc atcttcctga taactaaagg ctcgctgttc
     3901 ttcacttagg cgatatttgc gcatagcgtt ttcccacagg tgacttacta taaccgtagc
     3961 aaattctgcg gctctggcta tgctcgagaa aattccataa aatgcatttc aaatatactt
     4021 tataaattaa acaaaatgag taagaagatg cagagcgata aagtgctcaa tttgccggca
     4081 ggctactttg gtattgtgtt ggggacgata gggatgggat ttgcctggcg ctatgccagc
     4141 caggtttggc aggtcagcca ctggttaggg gatgggctgg tgattctggc gatgatcatc
     4201 tggggattat tgactagcgc atttattacc cgactcatac gctttccgca tagcgtgctg
     4261 gcggaagttc gccatccagt gctgagcagt tttgtgagtt tgtttccggc aacgacgatg
     4321 ctggtggcga ttggttttgt tccgtggttt cgcccactgg cggtgtgcat gttcagtttt
     4381 ggtgtcgtgg ttcagttggc ttatgccgcc tggcaaactg cgggattatg gcgcggatct
     4441 caccctgaag aagctaccac gcctggactg tatctgccga cagttgccaa caactttatc
     4501 agcgcaatgg cctgtggtgc gttgggctac accgacgccg gtctggtgtt tttaggcgca
     4561 ggcgttttct catggctaag ccttgaaccg gtgatcttgc agcgtctgcg cagttcggga
     4621 gaattaccca cggcactgcg gacatcactc ggcattcagc tcgctcctgc gctggtggcc
     4681 tgtagtgcct ggctgagcgt caacggcggc gagggtgaca cgctggcgaa aatgcttttc
     4741 ggttatggac tgctgcaact gctgtttatg ctacgtctga tgccatggta tctctcccag
     4801 ccatttaatg cttcattctg gagtttctcg ttcggcgtat ctgcactggc aaccaccggt
     4861 ttgcatctgg ggagtggcag cgataatgga tttttccata cgctggcggt gccgctgttt
     4921 atctttacca attttattat tgcaatactg ctcatccgta cttttgcgct tctgatgcag
     4981 ggaaaattgt tagtcagaac cgagcgcgcc gttttaatga aagcagagga caaagaatga
     5041 tcattcgtga cgaaaactat tttactgata aatatgaatt aacccgcaca cactctgaag
     5101 tactggaagc ggtgaaagtg gttaaaccgg gtaaaacgct ggatctgggc tgtggcaatg
     5161 gtcgtaacag tctttacctg gcagccaatg gttatgatgt tgacgcatgg gataaaaatg
     5221 ccatgagtat cgccaacgtc gagcgcatta aatccattga aaatctggat aatttacaca
     5281 cccgggtcgt tgatctgaat aacctcacat ttgatggaca gtacgatttt attctttcga
     5341 ctgtggtgct gatgttcctt gaggctaaaa ccatcccagg gttgattgcc aatatgcaac
     5401 gttgcactaa acctggtggt tacaacctga ttgtggcggc gatggatacc gctgattatc
     5461 catgtaccgt cggcttcacg tttgccttca aagagggaga attacgtcga tattacgaag
     5521 gctgggagag ggtgaaatac aatgaagacg tcggcgagct gcaccgcacc gacgccaacg
     5581 gtaatcgtat taaactgcgt ttcgccacga tgctggcacg taaaaaatga cccggtaagc
     5641 acaaaacgcg tgaaaattcc ccacgctgag atgatttact gttcttcttt tcggtaagca
     5701 tattttttat cgaagggatg tgaaattaat cacagtagtc gaagtttttt agcagcttaa
     5761 cttactgaaa tttaactact gatgattgac ttagcccctt tttcggcatt gactatgtcg
     5821 tctgaaaagg ggctgaaaaa tttattttca ccaacacttt ttttgccaca acacgaagcg
     5881 gcgctttttg ctataactta gaaagtaata taatcatctc aggaaactat tcatgcgtac
     5941 cacatcattt gcgaaagttg cagctttatg cggcttgttg gctctgtctg gttgtgcctc
     6001 taaaatcacc cagccagata aatattctgg ttttttaaac aattactctg atttaaaaga
     6061 aacaacctca gcaacaggta aacctgtttt acgttgggta gacccgagtt ttgatcaaag
     6121 caaatatgac agcatcgtct ggaacccaat cacttattat ccggtaccaa aaccttctac
     6181 ccaggtaggg cagaaagttc tggataaaat tttgaactat accaacaccg aaatgaaaga
     6241 agcgatagcg cagcgtaaac cagtggttac caccgctggg ccgcgtagtc tgattttccg
     6301 tggggccatt accggtgtag ataccagcaa agaagggctg caattctatg aagtggttcc
     6361 tgttgcatta gtggttgcgg ggacgcaaat ggctacaggc caccgtacca tggatactcg
     6421 cctctatttt gaaggtgagc tgattgatgc agcgactaat aaaccggtta tcaaagtcgt
     6481 tcgtcagggc gaaggtaaag acctgaataa cgaaagtacg ccaatggctt tcgaaaatat
     6541 taaacaagtt attgatgaca tggcgaccga tgccaccatg tttgacgtta acaaaaagta
     6601 gttccagacg cgccatcgtt agatggcgct ttttatccgg tgcgccgtaa aaccccatcc
     6661 ttcagggcgg ggatataagg cgcggttttc cacctgacca ggtgtttgct gt
//
LOCUS       DBKEKI010000187         6679 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.189, whole genome shotgun sequence.
ACCESSION   DBKEKI010000187 DBKEKI010000000
VERSION     DBKEKI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.189"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..276
                     /locus_tag="ACUQPX_003893"
     CDS             <1..276
                     /locus_tag="ACUQPX_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HHV9058402.1"
                     /translation="PAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIV
                     VMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            646..1470
                     /gene="gadX"
                     /locus_tag="ACUQPX_003894"
     CDS             646..1470
                     /gene="gadX"
                     /locus_tag="ACUQPX_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HHV9058403.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1838..2566
                     /gene="gadW"
                     /locus_tag="ACUQPX_003895"
     CDS             1838..2566
                     /gene="gadW"
                     /locus_tag="ACUQPX_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HHV9058404.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2929..5903)
                     /gene="mdtF"
                     /locus_tag="ACUQPX_003896"
                     /pseudo
     CDS             complement(2929..5903)
                     /gene="mdtF"
                     /locus_tag="ACUQPX_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5982..6239)
                     /locus_tag="ACUQPX_003897"
                     /pseudo
     CDS             complement(5982..6239)
                     /locus_tag="ACUQPX_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(6464..>6679)
                     /locus_tag="ACUQPX_003898"
     CDS             complement(6464..>6679)
                     /locus_tag="ACUQPX_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058405.1"
                     /translation="RLSSILQHEDQEAFEEIFKTSCFMPKKGLKVDKVVSMGWTSGVA
                     HVKVYVKGNLYDLWTNTENLQSEKAGK"
BASE COUNT         1764 a         1562 c         1572 g         1781 t
ORIGIN      
        1 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtacgacctc
       61 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
      121 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
      181 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgatcaccc gaaactgcag
      241 ggtattgccc agcagaacag ctttaaacac acctgataac ataacgttgt aaaaaccgaa
      301 tgcccagcct ttaaaaaaac agctgggcat tcggttgctt attaatcgca ataatatatt
      361 ggctgtctat tcatcgtgtt gataagatat atgttatatg aatgtttatt aaatgtagcg
      421 attaccttta atgtcacgaa aagggattat ttgtttgcta ttaatttcac tgcgttctta
      481 aacacgtttt tgtattataa atccagtcat cctgcccgta gaacatcaca cattatcatc
      541 ctgttctccc gcttcgttta aatttattta tcaatcaatt tgacttaaga gggcggcgtg
      601 ctacattaat taacagtaat atgtttatgt aatattaagt caactatgca atcattacat
      661 gggaattgtc taattgcgta cgcaagacat aaatatattc tcaccatggt taatggtgaa
      721 tatcgctatt ttaatggcgg tgatctggtt tttgcggatg caagccaaat tcgagtagat
      781 aagtgtgttg aaaattttgt attagtatca agggatacgc tttcattatt tctgccgatg
      841 ctcaaggagg aggcattaaa tcttcatgca cataaaaaaa tttcttcatt actcgttcat
      901 cactgtagca gagatattcc tgtttttcag gaagttgcgc aactatcgca gaataagaat
      961 cttcgctatg cagaaatgct acgtaaaaga gcattaatct ttgcgttgtt atctgttttt
     1021 cttgaggatg agcactttat accgctgctt ttgaacgttt tgcaaccgaa catgcgaaca
     1081 cgagtttgta cggttatcaa taataatatc gcccatgagt ggacactagc ccgaatcgcc
     1141 agcgagctgt tgatgagtcc aagcctgtta aagaaaaaat tgcgcgaaga agagacatca
     1201 tattcacagt tgcttactga gtgtagaatg caacgtgctt tgcaacttat tgttatacat
     1261 ggcttttcaa ttaagcgagt cgcagtgtcc tgtggatatc acagcgtgtc gtatttcatt
     1321 tacgtctttc gaaattatta tgggatgacg cccacagagt atcaggagcg atcggcgcag
     1381 ggattgccga accgtgactc ggcggcaagt attgttgcgc aagggaattt ttacggcact
     1441 aaccgttctg cggaaggaat aagattatag agttttactc agacataaaa aaaacccggc
     1501 ataggggacc gggaagagga tagtctgccg tctccagact aataaaccgt tataacactc
     1561 cctgttggca cgggaaactt tgtgctctca gtaagttaaa tataactttt actggaaata
     1621 agatcagcca tttttttata aacataagct atacgctgta cgaaaatata ttcgtgctgc
     1681 atttacttat tatcaattaa ctgttatgca aaactacttt gtggataaat tttggtccta
     1741 ccaaatctgg cagtttttgc gctaagaaac agtctggcat catttcatta gtatactgaa
     1801 attgaaataa tcgcagtatg aaatataagg gataatcatg actcatgtct gctcggtgat
     1861 cctcattcgt cgttcattcg atatttatca tgaacagcaa aaaatatcgc tgcataacga
     1921 gagtatcctg ctgctggaga aaaatttggc agacgatttt gcgttttgtt caccggatac
     1981 gcgacgactg gatatcgatg agctgacagt ttgccattac ttacaaaata ttcgtcagct
     2041 accacgcaat ttagggttac atagcaaaga ccgtctgtta attaaccagt caccccccat
     2101 gccgctggtg acggcgattt ttgatagctt caatgaatcc ggggtaaatt caccgatact
     2161 gagcaatatg ctctaccttt cctgtttatc gatgttttct cataagaaag aactgatccc
     2221 cttacttttc aatagcatca gcactgtttc aggaaaagtt gaacgcctta ttagctttga
     2281 tatcgccaaa cgttggtatc tgcgcgatat cgcggaaaga atgtatacca gcgagagtct
     2341 catcaaaaaa aagttgcagg atgaaaatac ctgtttcagt aaaatattac tcgcttccag
     2401 gatgtcgatg gccagacgat tactcgagtt acgtcaaatt cctctgcata ctattgcgga
     2461 aaaatgtggc tatagcagta cgtcgtactt tataaacaca tttcgacaat attatggtgt
     2521 aacgccacat cagtttgcgc aacattcgcc aggtaccttt tcctgacata ttttgcattt
     2581 gaatattggt caggatctca cacctgcttc atgtgaaact cttccctgat gatttctgcc
     2641 gggctaccgg ctagttctct ttcgcagttt gccaggcggt ataggtattt tcaggaggaa
     2701 aagactcggc atgtttggga ttattaagct gacaattcat acgattacgg gaatgtgggt
     2761 cagtattgtg ctgttcaaat tgatgacgaa tggctggagt ggtttctact ttcagtgctg
     2821 tgtactaagc ctggtattcc tgacggttag ctggttgttg agtggtgaat ggttagcagg
     2881 aaagagtaag gctgaacctt cacgttcaac cttactctca tttacacgtt acgctttttt
     2941 aaagcgggca aagagatgtt ccactacaac gaaaaagacc ggaacgaagt aaattgccag
     3001 cactgttgcg gcaaacatcc cgcccatcac gccggtacct accgcgtttt gcgcgccaga
     3061 accggcacca tgactgataa ccagcggcag cacgccgaga ataaaggcca gagaggtcat
     3121 caggattggg cgtaaacgca tccgcgccgc ttcgatgatt gcctctatcg gcgttttccc
     3181 ttctttctgc atcatctcaa cggcaaattc gacaatcagg atggcgttct tggcggaaag
     3241 cccgatggtg gtcagcaaac caacctggaa gtagacgtca ttacttaagc cgcgcagatc
     3301 ggtggccagt aatgcgccaa cgacgcctaa cggaacaacc aacatcaccg agaacggaat
     3361 tgaccagctc tcatagagtg cggcgagggc gaggaacacc acgaccagtg aaatggcata
     3421 cagtgcagga gcctggtttg aggataacgc ttcctgatac gacagtccgg tccatgagta
     3481 gccgacgcct gccggaagtt tagcgaccag gtctgccata aatttcatag cgtcaccggt
     3541 acttttcccg gccgccgctt cacctaaaat ctccattgac gggatgccgt tgtagcgttc
     3601 caatcgcggt gaaccatagg tccattcggt agacgagtag gcagaaagcg gtgccatcgt
     3661 gccagaggcg ttgcgtacat accattggtt gatgttatcc ggcaacatac ggaacggcgt
     3721 gcctgccggg acatacactt ttttcacccg cccctggttg aggaagtcgt tcacgtagct
     3781 gctgccgaag gcggtggaaa ttgtctggtt gatatcagac agcgccacac ccattgcctc
     3841 agctttcgcg gcgttgacgt tcactttgaa catcggcgta tcttccaggc cgttcgggcg
     3901 taccccggtg acctggtcag gcgattgcgc tgccagtgat aacagctcgt ttcgtgcctg
     3961 ggttagtttt tcgtgcccca ggttaccgtt gtccagcagt tccatatcaa aacctgacgc
     4021 ggtacccagt tcagccaccg cgggtaagtt gaacgggaag acgacggctt gagactgata
     4081 aacgccaggc cgttgttttg cccctggccg ctgaagccaa agccgccaac ggtaaacacc
     4141 gactgaacat tatctttctc ttttgtaaga taataatccg tcacctgttg cagtactttc
     4201 gtggtgttaa ccatggtggc accggaaggt aactgcgcgg tggtcataaa taccccctga
     4261 tcctcttctg gtaagaaaga ggtcggtgta cgcaggaaca gcaccgccat cccggcgcaa
     4321 atcagcaggt agaccaccat gtagcgaccg gtacaacgca acagcgagcg ggtgctatcg
     4381 gtatagtgct gagttgattt ttcaaacagc gtgttgaagc gtgcgaacag ggcgttaggt
     4441 ttgtgaccgc cttcctgcgc ggctttcaga atggtggcgc acagggcagg ggtcaggctc
     4501 attgccacaa atactgaaag cagcatggag gagatcagcg tgatggagaa ctggcggtag
     4561 atctccccgg ttgcaccgct cataaaggcc atcggcataa acactgcaga aagaacaacg
     4621 gcaatcccga ccagcgcacg ttggatctgc cccatcgatt tatgcgtcgc ttccttcggc
     4681 ggtagcttat cttccgcaat gacacgctcg acgttctcca ccaccacgat ggcgtcatcc
     4741 accagtaacc ctatcgccag caccatcccg aacatagtca acgtgttgat ggtgaaaccg
     4801 accgccgaca agatcgcaaa cgtcccgaga ataaccaccg gtacggcaat tgtcgggatg
     4861 atggtggcac ggaaattctg caaaaacaga tacatgacca ggaagacgag gatgatagcc
     4921 tcaaccagtg ttttgaaaac ttcctgaata gaaatttcga taaacggcgt ggtgtcgtaa
     4981 ggataaaccg tcttcagact tgcggggaaa taggctgata agcggttcag ctcctctttg
     5041 actgcccgcg aggtatccag ggcgtttgct ccggcagcca gtttgatggc gatcccggca
     5101 gcaggtttgc cgttatagcg cgccacggtg gaataatctt ccgccccaag ttcgacgcgg
     5161 gcgacatcac gcagcagcac ttgcgaacca tcttgctgaa ctttcaacag gattttgcca
     5221 aattcttccg gcgtttgcag acgcgtctgc acaatgatcg aggcgtttag ttgctggtct
     5281 gccgcctgtg gcatgccacc cagttgacca ccagaaatct ggttgttttg caccttaatc
     5341 tgggaaataa catcggaagg taccaggtta tatttattga gtttttgcgg gtccagccag
     5401 atacgcatgg cgtactcgga accaaagagc tgtacgctac caacgcccgc ggtacggctt
     5461 agcgggtctt tgatattaga cgctacatag tccgcgatat cgtactggtt gaggctgccg
     5521 ttatcagaaa taaacgccgc taccatcagg atattactgc tcgacttatc gacgctaatc
     5581 ccctgttgct gcaccgcttc aggtaatgaa ggcatagcga gttgcagttt attttgcacc
     5641 tgaacctgtg cgatatcagg agatgtccca gtctcgaagg tcagagtgat agaggcatta
     5701 cccgccgcat cactggttga agacatgtac atcaggccat caagcccatt catattttgc
     5761 tcaatcacct gagtgaccga gtcttctacc gtttgcgcat cggcacccgg ataggtagcg
     5821 ctgacggtaa tggtcggtgg cgcaatctgc ggatactgcg caaccggtaa gttcatgatc
     5881 gccagaccac ctgcaagcat cataataatg gcaagtacct aggcaaaaac cgggcgatca
     5941 ataaaatagt tagccatgaa agtcccctta agcctgcaac gttattgttt cgattcggtg
     6001 ctggcgtttt cctggctgga ggaaattgct cgtgctttga tacccggacg aatgcgttgc
     6061 aaaccggaaa cgatcacccg atcgccagcc tgcaagccag aggtgacaac ccactggtcg
     6121 ccgatggctt tgctggcttc aatttcgcgt agctgcacga catcgtcttt atccagaatg
     6181 agcgccgttg ctttaccctg ggcgttgtgg gtgacgcctt cctgcggcac cagtaatata
     6241 aagcgcgcaa aattattcaa gatatttttg aaaatcattc aagataggct atcgcgtgtg
     6301 atagcaaaaa cattcaagat aaaaatgctt gattattaag caatcaggaa tgggagtatt
     6361 aagtttcgtg tttccagtaa aaccagagag tattgaaata gctatcacct aactttatca
     6421 ggcgatagca ggtagatgat gtgattacag gttgactact gctttattta ccagccttct
     6481 cggactgaag attttcggtg ttagtccaaa gatcgtaaag attgcctttc acataaactt
     6541 tgacatgcgc aaccccactg gtccacccca tagaaaccac tttatcaact tttagcccct
     6601 ttttaggcat aaagcatgat gttttaaaga tctcttcaaa tgcttcttga tcttcgtgtt
     6661 gcaaaattga acttaatcg
//
LOCUS       DBKEKI010000188         6633 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.190, whole genome shotgun sequence.
ACCESSION   DBKEKI010000188 DBKEKI010000000
VERSION     DBKEKI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.190"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..658)
                     /gene="ipaH9.8"
                     /locus_tag="ACUQPX_003899"
     CDS             complement(<1..658)
                     /gene="ipaH9.8"
                     /locus_tag="ACUQPX_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HHV9058406.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     H"
     gene            complement(920..1015)
                     /locus_tag="ACUQPX_003900"
                     /pseudo
     CDS             complement(920..1015)
                     /locus_tag="ACUQPX_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1051..1356)
                     /gene="ccdB"
                     /locus_tag="ACUQPX_003901"
     CDS             complement(1051..1356)
                     /gene="ccdB"
                     /locus_tag="ACUQPX_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HHV9058407.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1358..1576)
                     /gene="ccdA"
                     /locus_tag="ACUQPX_003902"
     CDS             complement(1358..1576)
                     /gene="ccdA"
                     /locus_tag="ACUQPX_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HHV9058408.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2112..3204)
                     /gene="tnpC"
                     /locus_tag="ACUQPX_003903"
                     /pseudo
     CDS             complement(2112..3204)
                     /gene="tnpC"
                     /locus_tag="ACUQPX_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354984.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            3203..3781
                     /gene="parM"
                     /locus_tag="ACUQPX_003904"
                     /pseudo
     CDS             3203..3781
                     /gene="parM"
                     /locus_tag="ACUQPX_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
     gene            3784..4136
                     /locus_tag="ACUQPX_003905"
                     /pseudo
     CDS             3784..4136
                     /locus_tag="ACUQPX_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
     gene            4243..4380
                     /locus_tag="ACUQPX_003906"
     CDS             4243..4380
                     /locus_tag="ACUQPX_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058409.1"
                     /translation="MKLIHGAYEKAAPIIKIGEFAMSGNNVSDGLFASPERDIAASHE
                     R"
     gene            complement(4512..4787)
                     /locus_tag="ACUQPX_003907"
     CDS             complement(4512..4787)
                     /locus_tag="ACUQPX_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HHV9058410.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            complement(4787..5071)
                     /locus_tag="ACUQPX_003908"
     CDS             complement(4787..5071)
                     /locus_tag="ACUQPX_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HHV9058411.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDHPLPVPR"
     gene            5523..5774
                     /locus_tag="ACUQPX_003909"
     CDS             5523..5774
                     /locus_tag="ACUQPX_003909"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058412.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGSFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
     gene            5833..6033
                     /locus_tag="ACUQPX_003910"
                     /pseudo
     CDS             5833..6033
                     /locus_tag="ACUQPX_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(6088..6318)
                     /locus_tag="ACUQPX_003911"
                     /pseudo
     CDS             complement(6088..6318)
                     /locus_tag="ACUQPX_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6549..>6633
                     /locus_tag="ACUQPX_003912"
     CDS             6549..>6633
                     /locus_tag="ACUQPX_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HHV9058413.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKL"
BASE COUNT         1742 a         1545 c         1547 g         1799 t
ORIGIN      
        1 catgggatga taatgggtta tcactaatat gtattgaaca ttcattcctc agattaagaa
       61 tgctttccgg gatatgactt atctgatttc tatcaaaaaa atactctctg acaacgggat
      121 tatttccctc agaaaatgct ggtagctcag tgaggaaatt acgggtcgct ctgagatttt
      181 taagagcctg tgggagcgat ggtaaggaga ctatttcgtt atagctgata ttcatagtca
      241 ataacgaatc gggtaaagct ggcaggtttt ccagctcatt gtgttgtacc tgaagtgact
      301 ccagcgcagg aggtagcacg ggcaattctg ataatttatt gctggcggaa tataattttt
      361 ttagcgtaac aggcagttca ggtaggttag ttaattgatt atatgataca ttgagcagcg
      421 ttatctgagc tggtaagttg tcaggtagcg aggacagatt taaacggtcc agtcgaagtt
      481 catcggaatt attgataaga cattctttaa gtcgggagac agcctcatca cgctcttcac
      541 cggggagcgc ttgtttttcc catttatccc atgctgaaaa gtaatcagca tatgtaccgg
      601 aaatagtgtt ataaaaagaa ttttggggca atgaaaagtt attatttatc ggtaacatga
      661 tgggcatcca gttaaagccg gtggacaaat ggctttgtta aaagaaataa gtaggagttt
      721 ttatgaagtt cattccatga aatgaacttc agcacttttt tattataata aggataaaca
      781 aaattggctg taaaaaaacg atactgtttc agttaaccgt gaatggcctg tttgcaccgt
      841 attgctcttg tttttcggtt agttaaacat cgtacttcat atttgaacgg taagcggctc
      901 gtcagaaccg tattgatatt tactgagagg tcagatcaac tttccagggc aacagatcgc
      961 gtacccggtt tgccggccag tcctggatat gctcaatgac gtaacgcagc cattttgtca
     1021 acgccacgat ttttgaccct agcctgacat ttatattccc cagaacatca ggttaatggc
     1081 gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga taacgaagat
     1141 cggcacactg gccatatcag tggtcatcat gcgccagctt tcatccccga catgcaccac
     1201 cgggtaaagc tcacgggaga ctttatctga cagcaggcgg gcactggcca gggggatcac
     1261 catccgtcgt ccgggcgtgt caataatgtc actctgtaca tccacaaaca gacgataacg
     1321 actctctctt ttataggcgt aaaccttaaa ctgcatttca ccagtccctg ttctcatcgg
     1381 caaaagagcc gttcatttca ataaaacggg cgatctcagc catcccctgc tgattttcag
     1441 cctgccagcg ttccgcacgc agacggcggg cctctttttg catggccgta tttaccaggc
     1501 ctgagatatt cacgtttgcg gatttaagta actgatagct gtcactgtct atagtgacag
     1561 taatacgctg cttcatgata agccctcaaa aacatactta tgatatgtat atgagtatat
     1621 attaatggta caaaccatca atatccataa aaagtacacc attaattcgc tataagtttt
     1681 taatacaatt ttttctgctg cgattttttt atacgataat tagcatactt ataacaccgt
     1741 aagtaaatta catgaaagca ggcacagcgt gatccgattt ttctgcgtat aacgattttc
     1801 cggaagtgta atacggttgc gccccgggcc gggcaccctt ttctgttgtt gtgtacgggc
     1861 ccacagagaa tggtaccgga ggcgggctca gaagtactga gtcgggtgat cgcgtcaggc
     1921 cgcgaagtac tgacacgggt attggtgact ggtgatgaag tactgccccg ggtatgattt
     1981 caggctgatt caggttctga ttctctgtca gtccgcgtgg ttgctggcct agctgatttc
     2041 ctgttgtcag gcgttcaaca accgactgca ggttatagcc cggttcacga acacctgacg
     2101 gcaggtatca ctcacccggt gaaggtaaat ccctcaagag gcagcaactc tgccagtcgc
     2161 tcctccggcc attccggcag acgcgtcagg acgtccgtca gccaggcatg tggctccaga
     2221 ccattacgtt ttgctgtttc cagaaggctc attatctgtg ccgcccggtt tccggccatc
     2281 agtgaaccgg cgaacagcca cgctttccgt cccataacaa ccgggcgaat ggcacgctcg
     2341 cagatattgt tgtccagggg caccgctccg tcacccagga agcagctcag ttcatcgcgc
     2401 cggttcagaa tatagttaat cgccttattt aatggcccgt cgggcggaca gcacggctcc
     2461 tgctcttcaa gccaggcaaa caggtcattc actatcggcc gggagtaccg ttatcgccac
     2521 tgccgtattt tttccacagg gcgctcacgg atgagtttct caatgcggta aagacccgcg
     2581 atttttctca gctccacgcc tgcacggggc tccctgctgg ccttatacag atttgcgaag
     2641 ccgcgccctg cgtgactcca gcaacaggct gatgtgatgc cggggtgatt atccgccaga
     2701 gatttgtaca cgctgtatcc gtcactcacc agcgtaccgc cacaccagca ctgcagccag
     2761 gtctcagggt atctgagggc ccgtccggtc tgcgagtcaa aacagacgac cggcggaccg
     2821 cttctttcac cgctgacgta agcacacagc cagccggagc aggatttacc gcctttccgg
     2881 gtatcaagga gcctcaggga cgtttcatct gcatgaataa catcccgtgt cagaagctca
     2941 tggcgtagca gtttcgccag cggggccagt gcagctccgg caacaccgac catatccgcc
     3001 attgtactga cggggagctc cacacccatt cgggagaaaa tgtgctgctg gcgatacagg
     3061 ggcagatggt cggtatattt gctgatgaca acctgtgcaa ttaccgacga ctcaacaagc
     3121 actttcaggg aggatatgcg ccggcatttt cccgctgaac actttctcac agcagggaca
     3181 gctgtactgc ggacggatat agcggattgc attaaatggt ggggatacat tcacaataaa
     3241 agatgtaaaa gtcatgcctg aatctatacc ggcaggttat gaagttctac aagaactgga
     3301 tgagttagat tctttattaa ttatagatct cgggggcacc acattagata tttctcaggt
     3361 aatggggaaa ttatcaggga tcagtaaaat atacggagac tcatctcttg atgtatctct
     3421 ggtcacatct gcagtaaaag ataccctttc tcttgcgaga acaaaaggaa gtagctatct
     3481 tgctgacgat ataattattc acaaaaaaga taacaactat ctgaagcaac gaattaatga
     3541 tgagaacaaa atatcaatag tcaccgaagc aatgaatgaa gcacttcgta aacttgagca
     3601 acgtgtatta aatacgctca atgaattttc tggttatacg catgttatgg ttataggcgg
     3661 tggcgcagaa ttaatatgcg atacagtaaa aaaaaacaca cagattcgta atgaacgttt
     3721 tttcaaaacc aataactctc aatatgattt agttaacggt atgtatctca taggtaatta
     3781 ataatggaca agcgcagaac cattgccttc aaactaaatc cagatgtaaa tcaaacagat
     3841 aaaattgttt gtgatacact ggacagtatc ctgcaagggg aacgcagccg ccttaaccgg
     3901 gccgcactga cggcagatct ggccttatac agacaagatc cccggccccc ccatttcttt
     3961 tatgtgaact gctgacgaaa gaaaccacat tttcggatat cgtgaatata ttgagatcgc
     4021 tatttccaaa agagatggtc gattttaatt cttcaacaat cactcaatcc tcttcacaaa
     4081 aagagcaaaa aagtgatgaa gagacccaaa agaacgtgat gaagctaata aactaattca
     4141 attattattg agtttccttt atccactatc aggctggata aaggaaactt aatcaagtta
     4201 ttttcttgcc aatcattaca taaaataaac agggtcgtta ttatgaaatt aatccacggt
     4261 gcttatgaaa aagcggctcc aataatcaaa attggtgagt tcgcaatgtc tggcaataac
     4321 gtttctgatg ggttatttgc cagcccggag cgcgatattg ccgccagtca cgagcggtaa
     4381 ttttcgttat ttcatcgtaa tttattttat ctcctacaag attatttaat ggaatgcaaa
     4441 atatctggcc tgataacaat gctccagaat taatacctgc aggctatgtt aaagttgcct
     4501 ccacacccaa tctacctgtt ctctcgtgtg tgccacaggc gcaatacata aatagtctgg
     4561 ccagtaagtt cgtaacggat ttcgtactcg ccagtaaaaa tccgtctgac ctccctgggt
     4621 tcaaactgaa acaactgttc tcccatacgt ggatgagtta acagaatgac cggcgcctgt
     4681 gtcagggact gcaccgttct ggcggcagca ggtttactgg ccagcaccag aaaatcatat
     4741 aaccgcgcca aatcagaaag tgccttactg gtccacttca gttccatcag cgtggcaccg
     4801 gtaacggatg gtcagtactg aggctgtccg cccaggcctg tacagcctga tggtcgataa
     4861 cctgtccgga tgtcacatcg tccagggctt ccagcgtcag gcgattacgc tcctcctctt
     4921 gggcaagcca tgcagaaagc gcctgtttga taacccagcc ccgggagcgt tccagtctgg
     4981 cggccatctg gtcgacttta tcagccatag gtaatggcac atgcgcggta attacttttg
     5041 ttgcttgtgc ggtattgttt ttcatttgca tacccgatta gttgttaatc atatttaatc
     5101 atagtgatta ctttcagacg gaacaaggca aataatgcga gtcagcgcat ataaggcggc
     5161 atacggtggt tacatgtagc acaggtgtaa aaccgccgaa gcccggcgca agccggtacg
     5221 gtcgtccgaa tatcaccagt ccccctccgg ctctgcgagg ccatgcggcc tgtcagcgat
     5281 gaaaatgcct ctgtgcgcgg tcggagtggt cccgacaagg gtttacccgc agtcgaggcg
     5341 tatctccgcg ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagta
     5401 gttttgtccg ctcacaccag tacaaaccag tcgaaatacc ggtaacgacg attactcttg
     5461 tctctgatgc cggtggacta cgagatttta tttactggcg tcctgatgcg gtcaggactg
     5521 gtatggaacc tgtttatgtg atacttaatg ctcttctgga ttccgggcga tttacgcgta
     5581 aacttattct gttagggctg tcaggctcat tttcctatat cttcggctca attgttgcga
     5641 cactgggaat gggacttgtt gtcgattatc tgggatgggg ggccacattt atcgtactga
     5701 tactgtctgc tgtttttgcc atcattttca cactgatgag tcgtgaacgc tcactggagt
     5761 tcgaaaaaga gtaatttttt caggaaaaac ataaaattac tgactaaata tttatttttt
     5821 gaatcgcagg gcattccgga taaggttgat atcgtgtcac ggctgaagac ctatggatac
     5881 agtatttctg gtgtggagac ggatgacgga tataaggcgc ttgtccgcgc ttttcagctt
     5941 catttccggt agaaaaatta tgacggcata atggatgctg aaaccgctgc catcctgtat
     6001 gcattgctgg agaagtattt cccgggtaaa taaggagtga gtcgtcagag ctgcgatata
     6061 tccccttcct ccatgggctg tctcttatac acaaatcccc acccggggat ttgtgatgtc
     6121 agatgtcccc tgtaaaacta tgttttcatt gcgctctatg aagctgagtg agcgtaacga
     6181 ctggagctgc ttctgcggtg ctccggtggc gaacgtgaag tcatactctt cgaacatttt
     6241 caccgccggg aaggctacca ttcgggtata catcgcctgt ttacgttgat gacgtgccag
     6301 tttttcttca tgaagcatga tgcttcaaga agggcactgt tgcaaagtta tcgatgaggt
     6361 agctgaccat cactgctgtc ggaatcgatc ttgcccttct gtttccagtc gcctcagcaa
     6421 actatttttc agttaccacc tgtcctgcgg taagcgtaaa ccgaccgtaa gcgccccatc
     6481 agcgacgtct tgtgaaaatt gtcctgtctg gcaacaatcg cgcccatcta tattgatgga
     6541 cacgaacgat gaattcccag acaacaaaag atattccctg cttccgttct tatttgcctg
     6601 atgccctgcg tttaagattt gaagataaac tga
//
LOCUS       DBKEKI010000189         6606 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.191, whole genome shotgun sequence.
ACCESSION   DBKEKI010000189 DBKEKI010000000
VERSION     DBKEKI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.191"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..756
                     /locus_tag="ACUQPX_003913"
     CDS             <1..756
                     /locus_tag="ACUQPX_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HHV9058414.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSEAQHYDFIKKKSIYEKLNSKSSFD
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACUQPX_003914"
     CDS             complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACUQPX_003914"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HHV9058415.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHDYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSHSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQMGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1792..1995)
                     /locus_tag="ACUQPX_003915"
     CDS             complement(1792..1995)
                     /locus_tag="ACUQPX_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HHV9058416.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACUQPX_003916"
     CDS             complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACUQPX_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HHV9058417.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4588..5100)
                     /locus_tag="ACUQPX_003917"
     CDS             complement(4588..5100)
                     /locus_tag="ACUQPX_003917"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HHV9058418.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(5118..>6606)
                     /gene="hpaB"
                     /locus_tag="ACUQPX_003918"
     CDS             complement(5118..>6606)
                     /gene="hpaB"
                     /locus_tag="ACUQPX_003918"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HHV9058419.1"
                     /translation="TNGSVRYCQYTSDVTTHPAFRNAAASVAQLYDALHKPEMQDSLC
                     WNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAEWSRLSYGWMGRTPDYKAAFGCALGA
                     NPGFYGQFEQNARNWYTRIQETGLYFNHAIVNPPIDRHLPTDKVKDVYIKLEKETDAG
                     IIVSGAKVVATNSALTHYNMIGFGSAQVMGENPDFALMFVAPMDADGVKLISRASYEM
                     VAGATGSPYDYPLSSRFDENDAILVMDNVLIPWENVLIYRDFDRCRRWTMEGGFARMY
                     PLQACVRLAVKLDFITALLKKSLECTGTLEFRGVQADLGEVVAWRNTFWALSDSMCSE
                     ATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNIIERNVTSGLIYLPSSARDLNNPQID
                     QYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEFGGRHELYEINYSGSQDEIRLQCLRQ
                     AQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHNNDDINMLDKLLK"
BASE COUNT         1623 a         1712 c         1727 g         1544 t
ORIGIN      
        1 tatactaaga ttttagagaa tgattttgaa cacccttcaa tacgacgaaa aatagcatcg
       61 ctaagtaatt taggggtaga tgagtggatt cctccggtta tggcattcat gaatcgaatg
      121 gctcgtacag aagattttaa tttagatgat ttttctcaat ttattaccgc ctttgagaag
      181 gtatatatgc atgggtggtt aaaaaaacaa attaaaagcc aaagagaaat ggtttgttat
      241 tcagctctgg tagcaataaa taatgatatg ccttttgatt ctgtgataaa tcagataaac
      301 caacatgcag ataattcagg ttttatagct gctctggatg aagatttata tgagcctcgt
      361 ccgaatcaag tgaacttaat aaaagcaatt ttgcttcgct tagatatgga gcaacaagat
      421 gagagcgtga ttaaaaccta tacggggaga atcacaattg agcatattct tcctcaagca
      481 ttagttaatg aatactggat taatagattc caacctcaag agcatgtcta ctggctgcat
      541 aaaataggta atttaacact cataagtggt tccaaaaatt ctgaagcaca acactatgat
      601 tttatcaaaa agaaatctat ttatgagaaa cttaatagta aaagctcttt tgatttaaca
      661 aaagatgttt gtaattcttc agaatgggga ttggctgaac tgaaaatgag acatgaaaaa
      721 atgaaaacgc aattgaagaa gctttggttg gtctgacata tctaaagagt tccactcgta
      781 ataatgttgc ggtggaactc tcaaaaagtt gatctttccc gaacttactt cctcaatccc
      841 gcaaacgccg cccgaatctc ttcctccggc aactgaatcc cgataaacac cattgtgcta
      901 tgcggttttt catcgcccca cggcctgtcc cagtcggcgc tgtagaggcg ctggacgccc
      961 tggaacagca ggcggttagg ttcgccgtca atccacagca tccctttgta acgcagcagt
     1021 ttatccgccg attccagcag cagattttcc atcacgcggg aaacttcgct gatatctacc
     1081 gggtaatcca gttccaccac aattgacgaa atatcgtttt gtttatccgc gataaagtgg
     1141 aaacgcggtt tggtgctgac gacgttctct tccagcataa aaccgttggt gttgaacagc
     1201 aggcccaggt cgatgtcgcc gtgggtgacg gtgtagaccg gtgcgcgggc gttgatgcgc
     1261 gccaggcgtt cacgcagttt ctctgcttcg cctgcgacgt cggttttagt cagcagaata
     1321 cggtcggcgt agcccatctg ggactgggcg atggtgaact ggttcatctg ctcatcggca
     1381 tgtaccgcat ccaccagcgc aatcacgccg tccagcaggt agcgctggca taaaatctca
     1441 tgggagaaaa aggtctgaat aatcgggccg ggatcggcca tgccggtgca ttcaatcacc
     1501 agacgatcga actgaatatt gcccttgtcg agattatcca gcaggtcgag tagtgcgtcc
     1561 tccagctcgt tggagtgcga acagcagatg cagccgttgg tcagcgtttt gatctgcgtg
     1621 gcgcggtcgc caatcagttg atcatcaacg gagacttcgc cgaattcgtt ttcaatcacg
     1681 gcaatcttgt agtcgtgttg ttcgttaaga atatggcgca gcagggtagt tttgcccgcg
     1741 cctaaaaaac cggtgagtag ggtaactgca atcgggttca tgctctctcc attaacaaca
     1801 gcgcataccg cctttaccat cgccgccgta gcgcgcgttc tggcgttcgc ggaagaattc
     1861 ttcatagctc atgtacggct tgtcgggatg gttggtcttc atatgctcga cgtagttgtc
     1921 gtagtctgga ataccaatca acatcttcgc cgcctggccg agatattttt ttgcctgtcc
     1981 taagttacca aacatagttc actctgataa gaacaaagcc ccgccgaagc ggggctaaac
     2041 acggttagtg gtgcgaagag atcttcacgc cgccttccgg gattggaacg tacggtgttt
     2101 ctttgtcagt acgtttgtcg ctgttacgca ccgcaagcca ggttttgaaa ccgtagaaga
     2161 tgatgctgta caccacaatc aggaacagaa tactcaggcc tgcgttggtg tagttgttca
     2221 caacgatgtg gttcatgttg gcaatctgct gcgccgtcag gtcagtaccg ttagcaatct
     2281 tctctttgta ctggcttgcc atgtagaaga agccttccat ctgcgggttg gtgctgaaca
     2341 gtttcagacc cagcgcccag gtggtgcaga taagcagcca tacagccgga acaacagtta
     2401 cccagatgta ttgggtgcgc ttcatcttaa tcagcacaac ggtgcccagt accagcgcta
     2461 cggctgccag catctggttg gagataccga acagcggcca caggctctta acgccgccca
     2521 gcggatcgac cacgccctga tacagcaggt agccccacag acccacacag cccgcagtac
     2581 cgatgatacc ggcaaccaga gagtcggttt ttttcaggaa cgggatgaag ttacccagca
     2641 ggtcttgcag cataaagcgg ccagaacggg tacccgcatc cagcgcggtc aggatgaaca
     2701 gggcttcgaa cagaataccg aagtgatacc agaagcccat gtcagccatc ggcagcactt
     2761 tgtggaacac gtgagcgata cctaccgcca gcgtcggcgc accacctgca cggttcagga
     2821 cagaaggctc accaatgtct ttcgcggttt gcaggatctg ctctggcgaa atcacgaagc
     2881 cccaggagct gacggtcgct gccgcgtgtg cggtaacgtc tttcagctgc gccatgatga
     2941 tcggcgcgtt ctcgccaccc atttcatgca ggttaggcat ggtgatgcca aggccagcag
     3001 gcggggtgtt catcgcgaag taaagacccg gttcgatgat ggacgctgca accagcgcca
     3061 taatcgccac gaaggactcc atcagcattg cgccgtagcc aatgaaacgc gcgtcggttt
     3121 cgttagccag cagtttcggc gtcgtaccgg aagagatcag cgcgtggaag ccagataccg
     3181 caccacaggc gatggtgatg aacaggaacg ggaacagagc gcctttccac agcgggccag
     3241 taccgtcaat gtactgggtc atcgcaggca ttttcagttc cgggttcagc accacgatac
     3301 ccagcgccag gccgacgata acgccgattt tcaggaaggt tgccagatag tcgcgcggtg
     3361 cgaggatcag ccacaccggc agcagtgcgg aaacaaacgc atagccaatc agcgcgaagg
     3421 taatggtggt gtctttaaag gtcagtgccg gaccccagta cggatcgtga gcaatcacgc
     3481 caccgaagta gatagaggca accagcagca cgataccaat aacagagact tcacccacac
     3541 gccccggacg gataaagcgc atgtagatac ccataaacag cgcaatcggt acggttgagc
     3601 aaacggtgaa gacaccccac ggactttcgg ccagggcttt aaccacaatc agcgccagaa
     3661 cggcgaggat gatgatcatg attaagaaac agccaaacag cgcgatagtc cccggtactg
     3721 gccccatctc ttctttgatc atctcaccga gagatgcgcc gttacggcga gaggagataa
     3781 acagcaccat aaagtcctgt accgcaccgg ccagcactac ccccgccagc agccacagcg
     3841 tgccaggcag gtagcccatc tgcgcggcga gaaccggtcc caccagcgga ccagcaccgg
     3901 cgatagcggc gaagtggtga ccaaacaaca cgtaacggtt ggtcggaacg tagttcagac
     3961 cgtcgttgtt aataaccgca ggcgtcgcgc gcgtggggtc gagtttcatc accttctggg
     4021 cgatgtacag actgtagtag cgatacgcca ccagatacac cgacacagag gcgaccacga
     4081 tccacagggc gctgacgtgc tccccccgac gtaatgcaac taccgcgagg cagaatgcac
     4141 cgatgattcc gagaatcacc cagggtatgt gcttgaatag ttttttcgta tccatagtaa
     4201 aacctggcat tttactcgtc gtcttttggc ttacaggtgc ggcagctgcg ttcgttcacc
     4261 tctgacactt acttttgtaa gctcccgggg attcactcac ttgctgcctt cctgcaaacc
     4321 aaaatccata gagtaatttg ttaatcatag gccgaagccg ttctaggttt gcgttgcgtt
     4381 tgaggaggta aattgaccgc tatcctgcag tgatgttgcc agagatacgt gcgcgtaaag
     4441 taaggtaaat aactgagtgg ttattttagg gatgtaagcg gtcaggaatg gcggtgagcg
     4501 gttgggcaag aattattcat cgggtagata tgtgattgag atcacaataa aaatgcccct
     4561 tcgacgaatg cgtgaagggg ctggagctta aatcgcagct tccatttcca gcatcaccgg
     4621 atggaaacgg cgtttaaagt aaataagtcc gtgaccttct gcactgagga tgatgttttt
     4681 aatctccacc agatacacca gatgtgtgcc aatcgcctgc acatcgcgga tctctccttc
     4741 aagactggcc agcgaacctt ttagcaccgg ctgcgccagc ggaccttttt gccagcatga
     4801 gaggctaaaa cgctcttcca tcgccatgcc tgtcatgccc gcgaagtggc gcgccatcag
     4861 ttcctgctca tggttgagga cgttgacgca caacttgccg ttgccctgaa aaaccgggtt
     4921 catcgcactg ttggcgttaa tgcacaccat cagcgacggt ggtgtatccg tgaccgagca
     4981 gacagccgtt gccgtaatcc cgcattgtcc ggcgtcgccc tcggtggtga taatatttac
     5041 cgctgccgac aggctggcca tcgcgtcatg aaagcgcagg cgttgttcat ctaattgcat
     5101 cttaacctcc tgctgcgtta tttcagcagc ttatccagca tgttgatatc gtcgttgttg
     5161 tgcaggtgcg gcacagtcca gccgttctgg tcgtattccg acaggcagcg atcaaccatc
     5221 gccatcatct tgtccatatt gccggagctt tgtgcctggc gcagacattg caggcgaatc
     5281 tcatcctggc taccggagta gttgatttca tacagctcgt gacgaccacc aaattcgctg
     5341 ccaatggcgt cccacatcag tttgaggatc ttgatgcgct ggacgtgatc cataccgttc
     5401 gaaccgcgca catacttcgc cagatactgg tcgatctgcg gattgttcag gtcacgggca
     5461 ctggaaggga ggtagatcag gccactggta acgttgcgtt ctataatgtt tttgatcttc
     5521 gcgtaggcca ttggtgccag tacgcgatag gtttgcagtg cggcatgatc cggtaaataa
     5581 gccccgttga cccacggcgt cgcttctgaa cacatcgagt cactcaatgc ccagaaggtg
     5641 ttgcgccacg ccaccacttc accgagatcg gcctgcacac cacggaactc cagggtgccg
     5701 gtacattcga gtgatttttt cagcagtgcc gtaatgaagt cgagtttcac tgccaggcgc
     5761 acacaggctt gcagcggata catacgggcg aaaccgcctt ccatcgtcca gcgacggcag
     5821 cgatcaaaat cgcggtagat cagcacgttt tcccatggga tcagtacgtt atccatcacc
     5881 agaatcgcat cgttctcatc gaagcggctg gagagcgggt agtcgtatgg cgagccggta
     5941 gcacccgcga ccatctcata agaggcgcgg gagattaatt tcacgccatc ggcatccatt
     6001 ggcgcaacga acatcagtgc gaagtccggg ttttcgccca tcacttgtgc cgagccgaag
     6061 ccaatcatgt tgtagtgagt cagcgccgag ttggtggcaa ccactttcgc accgctgacg
     6121 ataatcccgg cgtcagtctc tttttccagc ttgatgtaaa cgtctttcac tttatcggtc
     6181 ggcaaatgac gatcgatcgg tgggttaaca atcgcgtggt taaagtagag gccagtttcc
     6241 tgaatacggg tataccagtt acgggcgttc tgctcgaact gaccgtaaaa gcccggattc
     6301 gcgcccagtg cgcaaccgaa agcggctttg tagtctgggg tacggcccat ccagccatag
     6361 ctcaggcgtg accattcagc gatggcgtcg cgttgctggc gcaggtcgtc ggcacttttc
     6421 gccacgcgga agaatttatg ggtatagccg ccgctgccgg tgtcggtgtt ccaacacaga
     6481 gagtcctgca tctccggttt gtgcagcgcg tcgtacagtt gggcaacaga cgctgccgca
     6541 ttacgaaatg ccggatgagt ggtgacgtct gaggtgtact ggcaatagcg gacactacca
     6601 tttgtt
//
LOCUS       DBKEKI010000190         6569 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.192, whole genome shotgun sequence.
ACCESSION   DBKEKI010000190 DBKEKI010000000
VERSION     DBKEKI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.192"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(64..1281)
                     /gene="ligB"
                     /locus_tag="ACUQPX_003919"
                     /pseudo
     CDS             complement(64..1281)
                     /gene="ligB"
                     /locus_tag="ACUQPX_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
     gene            1539..2162
                     /gene="gmk"
                     /locus_tag="ACUQPX_003920"
     CDS             1539..2162
                     /gene="gmk"
                     /locus_tag="ACUQPX_003920"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HHV9058420.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            2217..2492
                     /gene="rpoZ"
                     /locus_tag="ACUQPX_003921"
     CDS             2217..2492
                     /gene="rpoZ"
                     /locus_tag="ACUQPX_003921"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HHV9058421.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            2511..4619
                     /gene="spoT"
                     /locus_tag="ACUQPX_003922"
     CDS             2511..4619
                     /gene="spoT"
                     /locus_tag="ACUQPX_003922"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HHV9058422.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            4626..5315
                     /gene="trmH"
                     /locus_tag="ACUQPX_003923"
     CDS             4626..5315
                     /gene="trmH"
                     /locus_tag="ACUQPX_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HHV9058423.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAAQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            5321..>6569
                     /locus_tag="ACUQPX_003924"
     CDS             5321..>6569
                     /locus_tag="ACUQPX_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HHV9058424.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALYLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQ"
BASE COUNT         1610 a         1823 c         1701 g         1435 t
ORIGIN      
        1 ggaagccacg caagcacctt aaaatcacca tcatacacta aatcagtaag ttggcagcat
       61 taccgaagca aagtagcagg tcaacgagtt aaagcggttt tctggtggtg tcggttttgt
      121 acgttctgca ccgcgccaga ccacatcatc aatgcgagga ataccctgac cggcaaggct
      181 gacgaggatc tgatcaccag gcgcaatatc ccactcctgc cagcacctga cggaaccaat
      241 attcacccgc tggacttttt tatcatccag catgacaggt gcgagtgacg caaccaccga
      301 tattttaccg ctcttaccca ccgcaaattg aatcgccttc acttcggcaa cctgagctac
      361 aggttgatat ttccaggcca ccagccactc cgcctggccc ggtagccaat gacgggattc
      421 gggttcctta gcccctcgca caaccacgcc atcggtgacg aagggtaatt tcgctttcca
      481 ccactcattg cgtacgcgcg caacctcatc agcattttta accgcacggg tatacctctg
      541 cgttagagta aaacctgcgg tagccagctc ctttaaacga tcggtcatta actgcggtcc
      601 atccggccat gcccagacaa aaacagccag tgagtttagc gtgtcgctat tcccctggcg
      661 catcatcagg ccagcaactt ttgcgcgggc atttattccc cccatttgtt gttggatatg
      721 cccctcgcgc tggagaaata tttccccctg aagcgtactg ttggctaaag gcccgctaac
      781 ggtttgcggc acagcggaaa ttaagctaac tttctgcgtc cagtcctcgc ctttcaggcc
      841 attaccgcga ctgattgctt tgttcagttt cccgtcccga taaaccaggg ttaccgcaac
      901 gccatcaact tttggctgca cccaaagatc gctacgttct cgcatccaca gacttaatgc
      961 gtttttatcc gccattttgc gtacacccgt atgggcaacc ggatgcataa ccgcaccatt
     1021 taaaggcggc atcatcacgt cgcggggctc gctcacaaag cagcgttgcc actgcgtaag
     1081 acgggcgctt aactgatcgt aaacaccgtc ttccacctca ctttttcctt ccttccagta
     1141 gtcatcgtcc cactgtttta tttgctgttg caggcgggaa atttcttcct gtgctctggc
     1201 tggcgaccag gccggacaga ccgcccacac agatgattgc cagcacaaga tacttattaa
     1261 tatcgccatc catactttca tcttcacctc cgctgtagtc aggctggcag atatacaacg
     1321 tggtgaaggc aaagccgagt ggcaaaaacg gagtctgcga ggacgcttcc tgagaatcgt
     1381 ctttattgca gtaaatcaca ggcaaatgcg gaaacagcta cgcaaaacgc aacaactttg
     1441 cgcaaaaagt gtgagcaagg gctacgtcac atggccgcgc cgtgtataat aagctcgtat
     1501 gtaggcttta tttcgctaat cacatacgaa agatactcat ggctcaaggc acgctttata
     1561 ttgtttctgc ccccagtggc gcgggtaaat ccagcctgat tcaggcttta ttaaaaaccc
     1621 aaccgttgta tgacacccag gtttctgttt cacacaccac acgccaaccg cgtcctggtg
     1681 aagtccacgg tgaacattat ttctttgtta atcatgatga atttaaagaa atgattagca
     1741 gagatgcgtt cctcgaacac gcagaagttt ttggtaatta ctatggcact tcgcgtgagg
     1801 ccattgagca agtactggcg accggtgtcg atgtttttct cgatatcgac tggcagggcg
     1861 cgcagcaaat tcgccagaag atgccgcacg cgcggagtat ctttatttta ccgccgtcca
     1921 aaattgaact ggaccgccgt ctacgcggtc gcggtcagga cagcgaagag gtcattgcaa
     1981 agcgtatggc gcaagctgtt gcagaaatga gccattacgc cgaatatgat tatctgattg
     2041 tgaatgatga cttcgatacc gcgttgaccg atttgaagac cattattcgc gccgaacgtc
     2101 tgcgcatgag ccgccaaaag cagcgtcatg acgctttaat cagcaaattg ttggcagact
     2161 gaacctgatt tcagtatcat gcccagtcat ttcttcacct gtggagcttt ttaagtatgg
     2221 cacgcgtaac tgttcaggac gctgtagaga aaattggtaa ccgttttgac ctggtactgg
     2281 tcgccgcgcg tcgcgctcgt cagatgcagg taggcggaaa ggatccgctg gtaccggaag
     2341 aaaacgataa aaccactgta atcgcgctgc gcgaaatcga agaaggtctg atcaacaacc
     2401 agatcctcga cgttcgcgaa cgccaggaac agcaagagca ggaagccgct gaattacaag
     2461 ccgttaccgc tattgctgaa ggtcgtcgtt aatcacaaag cgggtcgccc ttgtatctgt
     2521 ttgaaagcct gaatcaactg attcaaacct acctgccgga agaccaaatc aagcgtctgc
     2581 ggcaggcgta tctcgttgca cgtgatgctc acgaggggca aacacgttca agcggtgaac
     2641 cctatatcac gcacccggta gcggttgcct gcattctggc cgagatgaaa ctcgactatg
     2701 aaacgctgat ggcggcgctg ctgcatgacg tgattgaaga tactcccgcc acctaccagg
     2761 atatggaaca gctttttggt aaaagcgtcg ccgagctggt agagggggtg tcgaaacttg
     2821 ataaactcaa gttccgcgat aagaaagagg cgcaggccga aaactttcgc aagatgatta
     2881 tggcgatggt gcaggatatc cgcgtcatcc tcatcaaact tgccgaccgt acccacaaca
     2941 tgcgcacgct gggctcactt cgcccggaca aacgtcgccg catcgcccgt gaaactctcg
     3001 aaatttacag cccgctggcg caccgtttag gtatccacca cattaaaacc gaactcgaag
     3061 agctgggttt tgaggcgctg tatcccaacc gttatcgcgt aatcaaagaa gtggtgaaag
     3121 ccgcgcgcgg caaccgtaaa gagatgatcc aaaaaatcct ctcggaaatc gaagggcgtt
     3181 tgcaggaagc gggaattccc tgccgcgtca gtggtcgcga gaagcatctt tattcgattt
     3241 actgcaaaat ggtgctcaaa gagcagcgtt ttcactcaat catggacatc tacgctttcc
     3301 gcgtgatcgt caatgattcc gacacctgtt atcgcgtgct cgggcaaatg catagcctgt
     3361 acaagccgcg tccgggtcgc gtgaaagact acatcgccat tccaaaagcg aacggctatc
     3421 agtctttgca cacctcgatg atcggcccgc acggtgtgcc ggttgaggtc cagatccgta
     3481 ccgaagatat ggatcagatg gcggagatgg gtgttgccgc gcactgggct tataaagagc
     3541 acggcgaaac cagcaccacc gcgcaaatcc gcgcccagcg ctggatgcaa agcctgctgg
     3601 agctgcaaca gagcgccggt agttcgtttg aatttatcga gagcgttaaa tccgatctct
     3661 tcccggatga gatttacgtt ttcacaccgg aagggcgaat tgtcgagctg cctgccggtg
     3721 caacgcccgt cgacttcgct tatgcagtgc ataccgatat cggtcatgcc tgcgtgggcg
     3781 cacgcgttga ccgccagcct tacccgctgt cgcagccgct taccagcggt caaaccgttg
     3841 aaatcattac cgcaccgggc gctcgcccga atgccgcgtg gctgaacttt gtcgttagct
     3901 caaaagcgcg cgccaaaatt cgtcagttgc tgaaaaacct caagcgtgat gattctgtaa
     3961 gcctgggccg tcgtctgctc aaccatgctt tgggtggtag ccgtaagctg aatgaaatcc
     4021 cgcaggaaaa tattcagcgc gagctggatc gcatgaagct ggcaacgctt gacgatctgc
     4081 tggcagaaat cggacttggt aacgcaatga gcgtggtggt cgcgaaaaat ctgcaacatg
     4141 gggacgcctc cattccaccg gcaacccaaa gccacggaca tctgcccatt aaaggtgccg
     4201 atggcgtgct gatcaccttt gcgaaatgct gccgccctat tcctggcgac ccgattatcg
     4261 cccacgtcag ccccggtaaa ggtctggtga tccaccatga atcctgccgt aatatccgtg
     4321 gctaccagaa agagccagag aagtttatgg ctgtggaatg ggataaagag acggcgcagg
     4381 agttcatcac cgaaatcaag gtggagatgt tcaatcatca gggcgcgctg gcaaacctga
     4441 cggcggcaat taacaccacg acttcgaata ttcaaagttt gaatacggaa gagaaagatg
     4501 gtcgcgtcta cagcgccttt attcgtctga ccgctcgtga ccgtgtgcat ctggcgaata
     4561 tcatgcgcaa aatccgcgtg atgccagacg tgattaaagt cacccgaaac cgaaattaat
     4621 gttttatgaa cccaacacgt tatgcacgca tctgcgaaat gctcgccagg cggcagcctg
     4681 atctgaccgt ctgcatggag caggtccaca aacctcataa cgtttctgcg attattcgta
     4741 ccgcagatgc cgttggcgta catgaagttc acgccgtctg gcctggtagc cgcatgcgca
     4801 ccatggcttc ggcagcggcg ggtagtaaca gctgggtaca ggtgaaaaca caccgcacca
     4861 ttggcgatgc cgtcgctcat ctcaaaggcc agggcatgca gattctggca acccatcttt
     4921 ctgataatgc tgtcgatttc cgcgaaattg attacactcg cccgacctgc attttgatgg
     4981 gacaggagaa aacgggcatc acgcaggaag cattggccct ggcggatcag gacatcatca
     5041 ttccgatgat cggcatggtg cagtcgctga atgtttccgt tgcctcagcc ctcattcttt
     5101 acgaagccca gcgtcagcgg caaaatgcag gcatgtacct gcgtgaaaac agcatgttgc
     5161 cggaagcagc gcaacaacgc ctgttgtttg aaggcggcta tccggtgctg gcgaaagtcg
     5221 caaaacgcaa aggcctgcct tatccccacg tcaatcagca aggcgagatc gaagctgatg
     5281 ccgactggtg ggctactatg caggctgcag ggtaagtgcc atgaaaggtc gcctgttaga
     5341 tgctgtccca ctcagttccc taacgggcgt tggcgcagca cttagtaaca aactggcgaa
     5401 aatcaacctg cataccgtgc aggatctact cttacacctt cccctgcgct acgaagatcg
     5461 cacccatctc taccccatcg gagaactact gccgggcgtt tatgccacgg tggaaggcga
     5521 agtgctgaac tgcaatatct ccttcggcgg tcggcggatg atgacctgcc agatcagcga
     5581 cggttccggc atcctcacca tgcgcttttt caatttcagc gcggcaatga aaaatagcct
     5641 ggcgacgggc cgccgtgtac tggcttatgg cgaagcaaag cgcggtaaat atggtgcgga
     5701 gatgatccac ccggaatacc gcgtgcaggg cgatctcagc acgccagaat tacaggaaac
     5761 gctcacgccg gtttatccaa caacggaagg cgtaaagcag gccacgctgc gtaaattaac
     5821 cgaccaggcg ctgtatctgc tcgacacctg cgccattgaa gaactcctgc cgccggaact
     5881 gtcacaagga atgatgacgc taccggaagc gttgcgcact ttgcaccgcc cgccaccgac
     5941 gctacagctt agcgatctgg aaaccgggca gcatccggcg caacgtcgtc tgattctgga
     6001 agaactgctg gcgcacaacc tcagcatgtt agccttacgt gccggagcac agcgttttca
     6061 tgcccagccg ctgagcgcca atgacacgct gaaaaataaa ctcctcgccg ccttaccgtt
     6121 caagccaacg ggcgcacagg cacgcgtagt ggcggagatc gagcatgata tggcgctgga
     6181 tgtgccgatg atgcgcctgg tacagggcga tgtaggttcc ggtaaaacgc tggtcgccgc
     6241 cctcgccgcg ctgcgtgcga ttgcccacgg caaacaggtg gcactgatgg caccaaccga
     6301 attactcgcc gagcagcacg ccaataactt ccgcaactgg tttgcaccgc tcggtatcga
     6361 agtgggctgg ctcgccggta agcagaaagg caaagcgcga ctgtctcagc aggaagccat
     6421 cgccagcggt caggtgcaga tgattgtcgg tacacacgcc atcttccagg aacaggtgca
     6481 gtttaacggc ctggcgctgg tgattatcga cgaacagcat cgttttggcg tgcatcagcg
     6541 tctggcattg tgggagaaag gccagcagc
//
LOCUS       DBKEKI010000191         6514 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.193, whole genome shotgun sequence.
ACCESSION   DBKEKI010000191 DBKEKI010000000
VERSION     DBKEKI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.193"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(102..1316)
                     /gene="rspA"
                     /locus_tag="ACUQPX_003925"
     CDS             complement(102..1316)
                     /gene="rspA"
                     /locus_tag="ACUQPX_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HHV9058425.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPLCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1522..1848)
                     /locus_tag="ACUQPX_003926"
     CDS             complement(1522..1848)
                     /locus_tag="ACUQPX_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HHV9058426.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1983..2324
                     /locus_tag="ACUQPX_003927"
     CDS             1983..2324
                     /locus_tag="ACUQPX_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HHV9058427.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2359..2919
                     /gene="speG"
                     /locus_tag="ACUQPX_003928"
                     /pseudo
     CDS             2359..2919
                     /gene="speG"
                     /locus_tag="ACUQPX_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2922..3632)
                     /locus_tag="ACUQPX_003929"
     CDS             complement(2922..3632)
                     /locus_tag="ACUQPX_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HHV9058428.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3740..4045
                     /locus_tag="ACUQPX_003930"
     CDS             3740..4045
                     /locus_tag="ACUQPX_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HHV9058429.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4244..5656
                     /locus_tag="ACUQPX_003931"
                     /pseudo
     CDS             4244..5656
                     /locus_tag="ACUQPX_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(6385..>6514)
                     /locus_tag="ACUQPX_003932"
     CDS             complement(6385..>6514)
                     /locus_tag="ACUQPX_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058430.1"
                     /translation="DLEILGKVFVVAEAATTVKMGTGSATAVLPVVKRLKFTKTEA"
BASE COUNT         1734 a         1660 c         1526 g         1594 t
ORIGIN      
        1 cttcgggata acgcttaccg ctcatgggca cctctcttta agccatctta aatgactctg
       61 aggtgtctgt taaacccgtg gcgattcaat tatcttactc cttacctgtt ccacagcgtg
      121 ccatcttcca gacgtgcgac tggtagataa gcaggttcat agggatattt cgccgccagc
      181 ttttcatcga attcgatacc aagacccggt ttgtctcccg gatgcatata gccgttatcg
      241 aaagtccagt tgtgcgggaa gacttcgagc atttgttcgg aataacccat gtattcctgg
      301 acaccgaaat tggggaccca cagatcaaag tgcagcgccg cagccatgca gagtggtgac
      361 aaatcggaag gaccgtgtga gccagtacgt acctgataca gcgaagcaaa atcggcaatc
      421 cggcgcatac cggtaattcc gcctgcatgg gtcagcgtgg tgcggatata atcgatgagt
      481 tgctcttcaa tcagttgttt gcagtcccag atgctgttga agacttcacc cactgcgatg
      541 ggtgtgacgg tatgttggcg aatgagacgg aagcattcct ggttttccgc tggcgtcggg
      601 tcttccatcc agaacatgcg ataatcttca atgcttttac caaagcgcgc cgcttcaata
      661 ggcgttaagc gatggtgcat gtcatgcagc aaatgttcat taaaaccaaa cttgttacgt
      721 accgcgtcaa acaatttcgg catgaaatcg aggtatttct ccgtcgacca cagctgctct
      781 tccggccact gtcctttggt tgcgggttca taagccagac ctttaccttt cgacatgccg
      841 taggtggttt tcataccagg gattccgcac tgcacgcgga tggctttgaa tcccagctcc
      901 tgatgacggg cataatcatc cagagcttca tcaatactgt gaccggtggt atggcaataa
      961 accatcaccc cttcacgaga cgcgccgccg agtaactggt aaagcggcat gttggcagct
     1021 ttggctttaa tatcccacag cgccatatca accgctgaaa tggccgacat cgtaaccgga
     1081 ccgcgacgcc agtaagcacc tttatagaaa aactgccaga tatcttcgat acggtgcgca
     1141 tcgcgaccaa taagccgcgg acaaagatga tcctgcaaat aagaggccac ggaaagctca
     1201 cgtccattga gggtggcatc cccaaggccc gtaataccgt cctcagtggt gatttttaat
     1261 gtgacgaaat tacgccccgg acaggtaaca aaaacttcag cctttacgat cttcatgttc
     1321 gattccttgc atcgcttgtc gtgatgcatg aaatctacgc aactgagcca ctgccataca
     1381 agtatgaaga tcgaaaaaag ccggagtgat cacaaaaaaa ggcgtatatt tgcgctgtga
     1441 atggttgaca aaagatgaaa tagaatacct tttgtcagct gacacttcct cgtatcttat
     1501 tgataaaatg gatttatgtt cctacgtgcg cccccagccc gcaacaatga tcaacattcc
     1561 gcaaagcgca atcaacgcac ccgtccagtc ataaagactt agtttcacgc catccacaac
     1621 gcgcagccac atcaatgccg tgcagacata aacgccacca taagccgcgt aaacacgccc
     1681 actcgccgca ggatgcaacg ttaacaacca gacaaacagc gccagtgaaa tccccgccgg
     1741 aagcaacagc cagatactgg cgtttcgttt taaccacaac cagggcagaa agcatccaat
     1801 aatttcacac agcgcagtag caaaaaatag taacgttgtt ttaatcatct ttgtcactta
     1861 ttgacatcat gtatagttat agggcgacat aatatcatca atataaacat cctcctggta
     1921 cgttttgcgt tcgcagtgga tggtgtagaa tcacctttaa tcattcatac agggaatgaa
     1981 ttatgaaaat cactctcagc aaacgaatcg gcctgcttgc ttttctgctg ccttgcgcac
     2041 tggcattgag cacaactgtt catgccgaaa ctaacaaact ggtgattgag tctggcgaca
     2101 gtgcacaaag ccgccagcac gccgctatgg aaaaagagca atggaatgac acgcgcaatc
     2161 tgcgccagaa agtgaataaa cgcactgaaa aagagtggga taaagccgac gccgcttttg
     2221 ataaccgcga taaatgtgag caaagcgcca acatcaatgc ctactgggag cccaatactt
     2281 tgcgctgcct ggaccgtcga actggccgcg ttattacccc ctaacctgtt attgatttaa
     2341 ggaatgtaag gacacgttat gccaagcgcc cacagttaag ctacgcccgc tggagcgtga
     2401 agatttacgc tatgtacatc aactcgacaa taacgccagt gtgatgcgtt actggtttga
     2461 ggaaccctac gaagcctttg ttgaactctc tgatctgtat gataagcgca tattcacgat
     2521 cagagcgaac ggcgctttgt ggtggaatgt gacggcgaaa aagccggtct ggtggagctg
     2581 gtggaaatta accatgttca tcgccgggca gaatttcaga taattatctc cccggagtat
     2641 caggggaaag gtctggcaac ccgtgccgcc aaattagcaa tggactatgg cttcaccgtt
     2701 ctcaatctct ataagctgta tctgatcgtc gataaagaga atgaaaaagc gattcacatt
     2761 taccgcaagc ttggctttac ggttgaaggt gaattgatgc atgagttttt tattaatggt
     2821 caatatcgta atgccattcg catgtgtata ttccagcatc agtatctggc agagcacaaa
     2881 acaccgggtc agactctcct gaagccgacc gcacaatagc attaataata atcgatcgta
     2941 tttttgatgg tgtaaacccg ttcgacggcg ggttttactc cttcatcaac aatgatcagc
     3001 tgacagtcca ccggattggc gtgactgtca tattcacagc tctgttttac attaccaact
     3061 cgttgattat ttagtaaagt aaccgctgtg taatctaatt ttttgatcgg atccgttgat
     3121 ggcgtggcgc tgacagataa tgttttgtcg ttacttttcg tggttttacc cagcggatac
     3181 ccctgatcat catagcgata ttccatctgc atttgtttgc tgctggcttt aatcacgaag
     3241 ccattatcat cggtttccca actcaccccg gcagaaggta attctgctag ctggcatttt
     3301 ccctgtaaac gtactctctt ctccagcgtc tcggcatcac ggtaataatt ggcgtccagt
     3361 accagagcga ccacggtatt attttccaga tccagtaatt cgagtgaatc aaaacagcct
     3421 tcttccgaca aagtcccaga aacacgtttc gtcacttcac cttgctcatc cattaatgtc
     3481 tgagtgaaat cttttaccgg accacgcagc ggatcaaaat cgaattcatt agagaaactg
     3541 gccatctcag gggtaaatga aagtgttacc tctgtgcggt cacatcctgt gaggaatatc
     3601 gcgagtaagc atggtagtaa tttgtatttc acaacagtca ccagagagta gagatgattc
     3661 tcaatcatag tagcaaatac agtactttac acgttaaatg ctatgcttaa agaagttatc
     3721 ttcgcgtaag gagcttatga tgaaactctc aacctgctgt gccgcgcttc tgctcgccct
     3781 cgcctcaccc gcggtactcg ccgcgccggg atcctgtgag cgcatacaga gcgatatatc
     3841 acagcgcatt atcaataatg gcgtacccga aagcagcttc acgttaagta ttgtacccaa
     3901 tgaccaggtt gatcagcctg attcccaggt cgtcgggcat tgcgctaatg atacgcataa
     3961 aattctctac aaccgcacca ccagcggtaa cgtctctgct cccgcgcagt ctacccagga
     4021 tggcgcgcct gccgaaccgc agtaacacat tatcgactga acgccggata tgacacatcc
     4081 ggcgatttga acatacaaca taatctcacc ttattactca tacccttcta ttgatgtgga
     4141 ttaataattc ttaacccaaa atgggtaggc tccctctatt gttagcgcgc taaatattca
     4201 atatataaac atttatataa cgataaagaa cagggagtga gttatgtcca aaaatgaccg
     4261 aatggtgggc atcagccgca gaacactcgt taaatcaacc gcaataggtt ctctggcgct
     4321 ggctgcaggc ggtttttctt taccgttcac cctgcgcagt gcagcggcag cggtacaaca
     4381 ggccagcgaa aaagtgatct ggggttcctg ttccgtcaac tgtggtagcc gctgtgcact
     4441 tcgtctacat gttaaagata atgaagtgac ctggttggaa actgacaata ccggcagcga
     4501 tgagtacggc aaccatcagg tacgcgcctg tttgcgcggt cgctccatcc gccggcgtat
     4561 taatcatccc gatcgcttga attacccaat gaaacgcgtg gggaaacgtg gtgaaggcaa
     4621 attcgaacgg attagctggg atgaagccct ggatactatc gccagtagcc tgaaaaaaac
     4681 cgtcgaacaa tatggcaatg gagctgtata tattcagtac tcttcgggga ttgttggcgg
     4741 caatatgacc cgctcttcgc catcagcctc ggcggtcaaa cgcctgatga actgctacgg
     4801 cggttcactc aatcagtatg gctcctacag caccgcgcaa atttcctgtg ccatgcccta
     4861 cacctacggc agtaatgatg gcaacagcac cacggatatt gaaaacagca agctggtcgt
     4921 gatgtttggt aacaacccgg caaaaacccg catgagcggt ggtggcatca cttatcttct
     4981 tgaaaaagcg cgcgagaaat cgaacgccaa aatgattgtt atcgatccgc gatataccga
     5041 tacggctgcc ggtcgtgaag acgaatggct ccctattcgc ccgggcaccg atgccgcgct
     5101 ggttgcgggt attgcctggg tattgattaa cgaaaatctc gttgatcaac cttttctcga
     5161 taaatactgc gtcggctatg acgaaaaaac cttacccgca gatgcaccca aaaatggtca
     5221 ctataaagcc tatattcttg gtgaaggtga cgataaaaca gcgaaaacgc cgcagtgggc
     5281 ttcgcaaatt accggtatcc cggtagaccg tatcatcaaa ctggcgcgtg aaattggcac
     5341 aacaaaaccc gcttatatct gccagggctg ggggccgcaa cgccaggcaa acggcgagct
     5401 gactgcacgc gctattgcta tgttacctat tttgacgggc aatgtcggca tcagcggcgg
     5461 aaatagtggc gcgcgtgaat cgacctatac cattacgata gaacgcctac cggtgctgga
     5521 taatccggtc aaaacgtcaa tctcctgctt cagctggacg gatgctatcg atcatggccc
     5581 gcaaatgacg gcactccgcg acggtgtccg gggcaaagat aaactggatg tgcccattaa
     5641 gttcatctgg aacttcaagg acttgaagcg gacccttagt aggcctgagg tcctgcattg
     5701 agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc attagacggg atgtctagac
     5761 ggctggtcag gacccccggt ggcctcggtc tggacttgag gtgggcctat ggggggaagt
     5821 ctgctggctt aatgtattag atggtcattc acatttttct gtcagaaacc atcggtccca
     5881 ctgtgagtcc aaacctaagg acctagaagt ccacccacag ccttagacag agtgtcgaac
     5941 acatccacac cgagtcgacc agagaggacc atgtagagac ccacagagag gaacacgagt
     6001 ttatagacca gagtcaggag ggacttcagg tctgcggaag tcatagacat aaagccccta
     6061 tctgttatgt tatattatta cattaacaac agatccagac cattggtcca gaccattggt
     6121 ccagaccata gttagttaat cacagtgata gcccaccacc tgaagtcctg ctgatattca
     6181 gtccagactt acctctgctc atggtcttac ctccctcggt ctcctgacct tgggtatcga
     6241 ctacagcttg ctgagcgtca tctggaggag ctacaggagg agctacagga ggcgggaccg
     6301 aagccccacc ttgagtccca ccaccacctg tcaggccctt ggcgatggac ctgaagcctt
     6361 tactaatgct cttacccatt ggtcttacgc ttcggtcttg gtgaacttca ggcgtttgac
     6421 tacaggcagg acggcagtag ctgagccagt gcccatcttg acggtcgtag cagcttcagc
     6481 gacaacgaac actttaccga ggatttccaa gtcg
//
LOCUS       DBKEKI010000192         6506 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.194, whole genome shotgun sequence.
ACCESSION   DBKEKI010000192 DBKEKI010000000
VERSION     DBKEKI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.194"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..313
                     /locus_tag="ACUQPX_003933"
     CDS             <1..313
                     /locus_tag="ACUQPX_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHV9058431.1"
                     /translation="VAMIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKT
                     HDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNV
                     R"
     gene            complement(389..853)
                     /gene="nanQ"
                     /locus_tag="ACUQPX_003934"
     CDS             complement(389..853)
                     /gene="nanQ"
                     /locus_tag="ACUQPX_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HHV9058432.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEADYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(850..1725)
                     /gene="nanK"
                     /locus_tag="ACUQPX_003935"
     CDS             complement(850..1725)
                     /gene="nanK"
                     /locus_tag="ACUQPX_003935"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HHV9058433.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1722..2411)
                     /locus_tag="ACUQPX_003936"
     CDS             complement(1722..2411)
                     /locus_tag="ACUQPX_003936"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HHV9058434.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2459..3949)
                     /gene="nanT"
                     /locus_tag="ACUQPX_003937"
     CDS             complement(2459..3949)
                     /gene="nanT"
                     /locus_tag="ACUQPX_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HHV9058435.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4059..4952)
                     /gene="nanA"
                     /locus_tag="ACUQPX_003938"
     CDS             complement(4059..4952)
                     /gene="nanA"
                     /locus_tag="ACUQPX_003938"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HHV9058436.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5074..5865)
                     /gene="nanR"
                     /locus_tag="ACUQPX_003939"
     CDS             complement(5074..5865)
                     /gene="nanR"
                     /locus_tag="ACUQPX_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HHV9058437.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            6245..>6506
                     /locus_tag="ACUQPX_003940"
     CDS             6245..>6506
                     /locus_tag="ACUQPX_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="YhcL; uncharacterized member of the DcuC family of
                     anaerobic dicarboxylate transporters; probable role in
                     transport of carboxylates across the inner membrane from
                     the periplasm; its encoding gene is not essential for
                     growth and is probably cryptic; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HHV9058438.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYA"
BASE COUNT         1637 a         1751 c         1623 g         1495 t
ORIGIN      
        1 agttgccatg atttacattg aacagcttga attaatccat aaaagtggtg atgtgttgta
       61 tccagtaaaa ataaccagaa aatcatcagg aaaaactgcc ttccaccttg ttccattcgg
      121 cttaaataag actcacgacc ttctagaggt ggaagatgct tcagaagcaa ttcggctggt
      181 gattgatgaa cgtcattcca ttcgctgcag tacacttaca gcaactatta cgaataaaaa
      241 gggcaagcga attaagcgga caggtatata cagtatcaag ggtgttaaca taaaagaata
      301 caacgtcagg taatggcaca gagcggtccg tcagttcagg tttggctctg tgccaaagat
      361 gggtcaggtc acacctgatc taataaattt aagccattaa atcagcctta accttcacca
      421 ccaccttttt aatctcaccc ggctcgccaa caacgcatcc cggcttatgc ggctctcctg
      481 gcataaacac ggcgaacatt cccggtttca ggatgatggt ttgctcgttt tcaatggcgc
      541 tacaaagctg gtaatcagct tcatggtgga actcttcaca ttggcgcgca gtgcctgcca
      601 tgccaaacag aatccgttcc tcaccgttta ataacagctg gatatcaatg tattgctcgt
      661 gcaattccgc ttttttctcg acgggcgatt gagtgttaaa cgtcatgaca ttcataaaaa
      721 tattgtcgcc ctgtaattcg taacgacccg gcgctttttc ttgcggtctg gcagctaagg
      781 ccagcgttaa cgcgtcctgt aacgcaggat gtaacccagc agacggtaat gactgtactt
      841 cacccatcat cataattttt ctccctgggc caacagcgca gccccaagta aacctgcatc
      901 atggcggtaa tgcgccgcca gtaaatcaac atgaaatgcc gctggctcct gcgccagata
      961 catttccacc aacgccagat acccttctgc cagaccaacg ctgccaccaa ccaccacgca
     1021 ctggcaatca gttgtggctt taatatcagc gatcagcctt gcaagcgtac gtgcggagcg
     1081 atgaatcagt tgctgcgcct gctcatcgcc ctgcccggcg cgtgtgaaaa tagttctcgc
     1141 atccgcgcca gccagctcgc cctgcgctgc cgccgcaatg ccgcgaccag aagcaattgc
     1201 ttccacgcaa cctgtgcgtc cacagccgca gactgggccg tgtggatcgg caagcgtatg
     1261 cccgatgtgc cccgccagac cgccagggcc ggtaagcagt ttgccgccgc tcactacacc
     1321 gccgccgacg ccagtggaaa cggtgataaa gaccatatcg gttatatcgc cttccagcgc
     1381 ctgatactct gcccatgctg cggcctgcgc gtcgttaatg gcaatggtcg gcaaattggt
     1441 aagttgttcc agcgttttga ctaacggaaa gtgtagcaat ccaccgagat tatgcggatt
     1501 cagcgccagc aaactgccgt cgcgaataat tccggttgaa gcgatggcaa cccgctgcgc
     1561 atgagcttgc aacggagaga ctaatgcgga taaggcatca cgcaaggctt ctggtgtctg
     1621 gctggctggc gtaggaagtt cacgacgatc gcggatctgc ccgtcagcgc caatcagcgc
     1681 ggcggcaagt ttagtaccgc cgatatcaat cgccagtatg gtcatagcac cgcctttttc
     1741 atcgctgtgt tgtaccactg acaaatgtgt tcaagacgcg taatggcaga accgaccgtc
     1801 accgcccacg cgccgtggcg catcgcatct gccgcctgag caggcgtgtt gtaacgccct
     1861 tcggcaatca cccgacatcc ggcttcgctc aacgttttca ccagcgccag atccggctct
     1921 tctggcgttt caggcgtggt atagccagaa agcgtagtgc caataatttc ggctcccagc
     1981 ttttggcatg ccaggccgtc ttccggcgtt gagcagtcgg tcatcgccag taaaccgtga
     2041 tgatggatac gtgccagcag cgtttcaaca ggcaccggac gcgggcggtc ggtgccgtca
     2101 atggcgataa tgtccgcgcc cgcctgcgcc agcgcatcga catcttcaat ataggccgtg
     2161 atgcgtaccg gagaatcctc cagatcgcgt ttcacaattc caataatcgg cacgctcacc
     2221 accgcacgcg tggcttgcag atttgccaca ccttcgatgc gaatggcaac cgcgcccgcc
     2281 tgctctgccg ctaatgccat ggctgcgacg atttcgggtt tatcgagcgg gctgtcagga
     2341 accggctggc aggagacaat caggccaccg ttagcagcga ttttttgatc cagttgtgca
     2401 agtaacgaca tacatcttcc cttagcgaaa ggcccggtac atagaccggg caacaggatt
     2461 aacttttggt tttgactaaa tcgtttttgg cgctgccaaa tggcacggca ccgctaaatg
     2521 gtttgccgtc gatagcgtca tgagtacgca atgcttccgg gcgcagccaa cgctgaacgc
     2581 gggaaggcat atccagacca atcagcagga tcaccacaaa tgtcaggctg aacgagagcg
     2641 atgccagcgc agtacccaga tccagacgtt gagcgatcaa cgcgccgatg attggggcca
     2701 gtgcgccacc caatgcgcca acgttgtagg taaagcccag gcccgctgca cgctggtcgg
     2761 tatcgaaata accgccaatc agtttcggta agatcccggc gatcccttgt ccaagcattt
     2821 gctggaagaa cagtaacaga ccgagcaccc agacgtttgc gccgccaatc gcaaataccg
     2881 gaataatcag cagctgcgag gccagcaggc tacaaacgta cgctttgcgg gttcccagcc
     2941 agtcaccgag gaagccacct acgcagcatc ccacggccgc gccaaagcca ctaaagaaca
     3001 gcacattggc gacagtatgc gggttataag ccagatcggt tttcagatac gttggcagca
     3061 gcgcctgaat cggccatgag tagaggaaag caaacaacac gaccaccatc agcattacgc
     3121 ccgttggcca gcgtttaccc gtactttgca ccataaagct gataaagatt gcggcgcata
     3181 acagcccaag aacagcgacg attgcagcat tttgcaggtt accggcgaag cagaaccaca
     3241 gcgcagtagc cgccgccagt gtcattacga tattggcaat gcgatgttca ccacggtaga
     3301 gaatatccac cattgtgcgt actggtgctt tacctgcgtg tttctctttc cagtcttccg
     3361 cttccgggat gtttttacgc agccagagag caaagatgat tggcaaaatg ccgataaaga
     3421 acagcgcacg ccaaccccag accggaacca ccaggctata gacctgagcg gcaacgacgg
     3481 cccccacaga gaagcctgaa atcaaaaaac cactggcttt gttacgcaga tgttttggcc
     3541 agctttcaat gacataggtg gcgctggaac cgtattcacc cgccatcccc atgccgatga
     3601 ccagacgagc gataaacatg gtgatgtagc ctggcgcaaa gccgcaggcc agcgtcccgg
     3661 ccgagaagag aacgatgctg gtgaccattg ccagacgacg cccgtagcgg tcacccatag
     3721 cgccgagcat caggccgccg aaccagcgag agataaaggc tgcggagatc agacttgccg
     3781 cctgcaccgt cgtcagcccg aattcaccct gtacttcggt gagtaccagg gcgattaaaa
     3841 cgaaatcaaa accgtcaagc agatatccca accaggcagc ggaaaatgcg cgccattgtg
     3901 cacggttgag atggcgatac cacgggatat tctgggttgt agtactcatt gtgagtctcc
     3961 cgcggtgggc gatgcccaca cgctttggta tgaaaattgt agggtacaga tgcgtttatt
     4021 tcccctcacc cggtaggggc gagcgagggg aaaacaactc acccgcgctc ttgcatcaac
     4081 tgctgggcca gcgccttcag ttctggcaga tatttttcat ctaccggtcc aaacggtttg
     4141 cggcacagcg gcacagaaac gacatccata taatgcagga cagttttcag gccgcggaat
     4201 acgcccgttt tgatcagtaa atcaatgact ttgttgcact cagtttgcag tttctgcgcg
     4261 gtctggatat cgccttcttt cagcgccttc acgatcccct gatatcgcca gcccatgatg
     4321 ttgtaagtac tgccgatacc accatcagcg cctgccagca agccggaagc gaagatttcg
     4381 tcgtaaccgt tatagagcac cagatcagga tgttcacgac ggatctgttc catctgatag
     4441 agatcgccag aagtctgttt cagcgcaccg acgccaggca acgtaaccag ggtgttgatc
     4501 tgatccaggg tcagttttac cccactcagg gctggaatgt tgtacaccac catcggcaaa
     4561 ccatccgctg aatcaataat tgcccgatag tgatcgcagt gttcttcaaa gctgaaagga
     4621 tagtagaacg gcgtgacggc agagacggca tcgaagccat aacgtttagc cgatgccgca
     4681 agttgttggc tttcggcggt gctgacgcaa ccgacgtggg cgatgagttt aatcttacct
     4741 ttcgcctctt cggcgacgat ttccagtacc tgttcacgct cggaaaggct ttgtacaaag
     4801 gcttcgccgg tcgaaccacc cacgtataaa ccgtcgatgc cctgctgaat attgaactga
     4861 accaggcggc gcagactcgc tttatccagt gcttgttgtt gatcaaaagg agtcaggagt
     4921 gcagccatta cgccacgtaa attcgttgcc ataaatacct ctgaagtgat gcttgtctga
     4981 taaacgatat acctttatac ctgttatacc agatcaatta agcaacaccc catacagaaa
     5041 gcttataatg cgatctgctt cactaaagtg gcattatttc tttttgttgg tggtctgacc
     5101 gaaagcgtgc caggtagcag agacgctgtt gagatgcgat tgcaacgcac gatcggcttc
     5161 gtcaggatca tgacggcgga tcgcatcaac gatcgcaata tgctgttgat aactaacgtt
     5221 gttatgttcg tgcagtgcct gatcggtaac cgttgggcgt gcggcaataa gccagtcgag
     5281 cagggcaacg tggatcgcca tgaagattgg gttaccgggg atctccgcca gcacgcggtg
     5341 gaaatcaacg tctgaacgaa tgaatgccgc gttgttatcc agcgactgac tgttgatttc
     5401 cagtgctttt gccagcaaat cgatttgctc atcggtggcg tgttcagccg catagcgcac
     5461 caggctggat tcaaagaaca gacgtaattg ttcgaaatgg gcaatcccac caggatgaga
     5521 aaggaaatct ttcgccatgc cggaaagctc gccgatgatg gtgtccgcag aaggtcgaga
     5581 gacacgagcg cgttcgccgt tgtttatttg caccagacct ttgcgtttta acgctgccag
     5641 cgcttcacgc acagaaggac gcccgacgtt aaagaacgcc atcagttcgc gttcagacgg
     5701 taattgttca ccttcgccaa attcacgacg gcggatcatc tgttccagct cttcttccac
     5761 catttcggag agttttttac gcgccagcgg gcggctacgc aagttgcgac caattgcagg
     5821 tgaagaatct tcggtttgcg aatcaaatgc attcataagg cccattctgt aaggtcagtg
     5881 tgattaacat catcagtgac atcctatcac aggattgaaa gtaggggaaa atggcaggtt
     5941 tttctctttg tgcctcatca ttaccataat taacggaata attaactatt gcgaaaaatt
     6001 aatgtaacgc agataaacac atcccgattg aattattcat aagactattc acaagcatta
     6061 tgaatattat gaatgtgttc ttacaaaata atcataatcg catatttttt aatgaaaaat
     6121 cacctcacct acaattaaaa acacgatatc cgcaccataa atagacttgc aaaaaatata
     6181 acatcattgt tttcaatctg ccgtttatgg gattgaccgt tttcttttga cacggagttc
     6241 aacaatgttc ggcataatta tatctgtcat cgtattaatt acgatgggct atttgatcct
     6301 gaaaaactac aaacctcagg tggtgctggc tgccgcaggt gtcttcctga tgatgtgcgg
     6361 agtctggtta gggttcggtg gtgtactcga tcccgccaaa agcagcggct acttgatcgt
     6421 cgacatttat aatgaaatcc tgcgcatgct gtccaaccgc attgccggat tggggctgtc
     6481 gattatggcg gtgggcggtt atgccc
//
LOCUS       DBKEKI010000193         6430 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.195, whole genome shotgun sequence.
ACCESSION   DBKEKI010000193 DBKEKI010000000
VERSION     DBKEKI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.195"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            9..161
                     /locus_tag="ACUQPX_003941"
                     /pseudo
     CDS             9..161
                     /locus_tag="ACUQPX_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1542..2489
                     /gene="icsP"
                     /locus_tag="ACUQPX_003942"
     CDS             1542..2489
                     /gene="icsP"
                     /locus_tag="ACUQPX_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HHV9058439.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3050..3422)
                     /locus_tag="ACUQPX_003943"
                     /pseudo
     CDS             complement(3050..3422)
                     /locus_tag="ACUQPX_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3644..3772
                     /locus_tag="ACUQPX_003944"
                     /pseudo
     CDS             3644..3772
                     /locus_tag="ACUQPX_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4022..4888
                     /gene="ospB"
                     /locus_tag="ACUQPX_003945"
     CDS             4022..4888
                     /gene="ospB"
                     /locus_tag="ACUQPX_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HHV9058440.1"
                     /translation="MNLDGVRPYCRIGNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5217..5957
                     /gene="phoN2"
                     /locus_tag="ACUQPX_003946"
     CDS             5217..5957
                     /gene="phoN2"
                     /locus_tag="ACUQPX_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HHV9058441.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            6030..6152
                     /locus_tag="ACUQPX_003947"
     CDS             6030..6152
                     /locus_tag="ACUQPX_003947"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058442.1"
                     /translation="MGTPRFTLEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(6176..6430)
                     /locus_tag="ACUQPX_003948"
                     /pseudo
     CDS             complement(6176..6430)
                     /locus_tag="ACUQPX_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
BASE COUNT         2024 a         1207 c         1265 g         1934 t
ORIGIN      
        1 gttcctaagt ggaatgtctc cacggagaac actttatcta tcgggaaata gtgcgggcaa
       61 cagtgtttaa ttatatcgag tgcgattaca atcgctggcg acggcaccgt tggtgtggcg
      121 gcctcagtcc ggaacaattt gaaaaccaga acctcgctta ggcctgtgtc catattacgt
      181 gggtaggctc acagagcctc acttacgact gcgtccatat tatgtgggta ggatcaataa
      241 agacgtttta tatgtgtctg cttgcagcca gaaaatttat accctgcagg tatcataaac
      301 atgcttgata acttaattgg ggctccttct tccttttatg gtgtaattgt ttgattgaat
      361 acttccgggg atttcagtat gaaatgaagt atatttaata tactttacaa aaagtttgta
      421 ttagtggaat aaggacaata gcttatccaa actgccaagc ctctttatta taagtaagat
      481 ctggcactgg aatattctca aaaggactaa catcatcacc acgaccttca accaaccatt
      541 ttattgcatt taaatcccag aatagttcag tagtggtcaa tttgggaaac gcacaatatt
      601 gtacggttac gttatcgcaa tagagtgaac tcccgcccta tacggcgttt tcaaggatta
      661 gtccttaatc ggacaaccac attaaagcat aataaataat taagctttaa taatatacta
      721 tagatacagt tctattataa aaaatactat tttaggtgat gcattaaatt agtagaatat
      781 ttattctttt ataaaagcac caatgaattg tccaagtaaa catgaggtaa aatgtgtaga
      841 ggataactga gtttggtcac tttaactgta ttagtcgcag tctgactctc gactttaaaa
      901 ggatgggaat tacctgtact gatagtgaga aagctttcca ttgaaataag tcaaataaaa
      961 gttgatggct tcagtgctaa agtgaggtgc ccgctagccg tattcactcg aatcggaatt
     1021 caatgtattg cttctgccat ttccataata tttcacggga aaaacaaaat ctgaaaaact
     1081 atacttaagt gttgtattga gtcagttgag atacaaatac atatttatca gcacacgtta
     1141 cgtcaaatac aggaggttgt catgttggta ctgaaaggca cgttggctat tgcagccgtg
     1201 atgacaattt attgtcttat cgttgttctt atggaacgct tttctgattg atttcatatt
     1261 ggcgagagaa caagaaggtg agtagaattg tcgcggaatc ctgaaactat cagcctcagg
     1321 tatgaacacc aaaaggtagg tagagaaatc tatggccccc gttactttat tcgcacaatg
     1381 aggtattttt ttatttttac aaaagttcaa taaatattat aataaaagat atgttcttgg
     1441 taacataaac aaatatttaa ttaaaataat gcttggcatt gacgtttatc ttataaagta
     1501 agaagatcat ggacatttca accaaaaaag tagagttctc gatgaaatta aaattccttg
     1561 tacttgcact ttgtgtacct gcgatcttta ctacacatgc taccactaac tatccacttt
     1621 tcataccgga caacatcagt acggatatta gtctaggatc tctgagtggc aaaacaaaag
     1681 aacgcgttta tcatcccaag gaagggggga gaaaaattag tcaactggac tggaaataca
     1741 gtaatgcgac tattgttaga ggtggcatcg attggaagct aattccaaaa gtgtctttcg
     1801 gagtttccgg ttggactact ttaggtaacc agaaagcaag catggttgat aaagactgga
     1861 acaattccaa tacccctcag gtatggaccg accagagctg gcatcccaat acgcatctcc
     1921 gtgatgctaa cgaattcgag ctgaatctta aaggttggtt attaaataat ttggattatc
     1981 gacttggact aatagcaggt taccaagaga gtcgttacag ttttaatgca atgggaggga
     2041 gttatattta tagtgaaaac ggggggaaca ggaataaaaa aggggcacat cctagtggtg
     2101 aaagaacaat aggttacaaa cagctcttca aaatacctta tattggatta actgctaatt
     2161 accgccatga gaattttgag tttggagcag aactgaaata tagtggttgg gttcgttcat
     2221 ctgatacaga caaacactat cagactgaga caatttttaa agatgaaata aaaaaccaaa
     2281 attactgctc tgttgctgcg aatattggat actatgtcac ccccagtgca aaattttata
     2341 tagaaggctc cagaaattac atttctaata aaaaaggtga tacatctctt tatgagcaaa
     2401 gtaccaatat atctggcacc attaaaaata gtgcaagtat tgaatatatt ggttttctca
     2461 cttccgcagg tataaagtat attttttgaa ttggttcacc gagttgatta cgttttcatg
     2521 gctaaaaatt gccgcccatt gtgagtcata ttcgccctga ctttccagaa ccatacgaac
     2581 tgcccgttga cggacttcgg gggaaaaacg tgtattttta gtcattctgt ttacctcttt
     2641 ctcagggcgt ttagtctcca ggatttccgg ggcggttcaa aaaactcttc gttgattact
     2701 gtggacttac tgttggcatt accgaccctg agaccggaga aataagaacc gcttagatca
     2761 tcgtggctgt tctcggggta tcaagttaca catgggcaga gcctacccaa ggtctactat
     2821 tatcgcctga aagcgctgat ggaacagtgc tatcagggac atgctgatga aaatacctaa
     2881 ataatcagcc agcactctat ctttccaaat ccacagcata gcaaagagag caaaagagcc
     2941 tgtaaattca gaaattaagg aaagcctctt ggtgcacata atctatctta tgttaaatga
     3001 aatctaattt cttgattata tgctggatcg ttcgtgggtg gctttgcccc tatatttcca
     3061 gacacctgtt atcattggcc cgctgccgca gatattcccg tggcgagcga taatccagcg
     3121 cactatgcgg atgccattcg ttataatgct cgaatgcctc tgcaaggttc tttgctgccg
     3181 ttaacccgtc aggtttgggc atgatactga tgtagtcaca ctttattgtt ttcacgaagc
     3241 tctttgctat tctgttactc tccggactcc gcaccgccgt gctcttcggt tcaacaaagt
     3301 cccaacatcc gggtgaactg gcgtgtttca ttagcccggt agcatgaacc attatccgtc
     3361 agtcacttca ctggagacgc cggaagctcg ttgccgaagc ggtgttccac cgctcccagc
     3421 atgtcgccaa cgggagagca tctacggtct gattgaactg tttattcaaa ttgtccaccg
     3481 tctgtcagta cgctctgaac gtcggctggt taaaacactg ctcgctgatt ttcagagggt
     3541 tcacggtaaa acagcacttt tattccggat tgcggaagcg gctctgaata atccggatgg
     3601 gctggttaaa atctgcatat tgttgaacca gatcgcagcg gtaagagaag ttcatcatac
     3661 ttggcgcagt tacgcgatgt ttctgctaga ggtgatgccg taaagaacgg cagaacatta
     3721 ccggaacaag atagcggtct acctgcactg gtatcagacc cggggcttcc ccggatgatc
     3781 tccacggtac tttaggtact gtgtctatat tatgtaggta ggatcaatta actttgtagt
     3841 taaaattctt tcattgaaat aatgaataaa aaagacaaac cgcattaaac aatgccagaa
     3901 tataattgca tttttgttcc gtttttttaa atcatagtat acaaagaagc ttgcatacta
     3961 tatgaaaata taacaccctt tactatgatg tttataaaac aatatatgga gtcatgtagg
     4021 tatgaattta gatggtgtta gaccatactg tagaataggc aataaaaaga atgaaagcat
     4081 atcagatatt gcatttgcac atataataaa aagggtaaaa aattcatcat gtactcaccc
     4141 aaaagcagca ttggtttttt taggagagaa aggtttttgt gatagcaatg atgttctatc
     4201 tataatggga caacaaatac caagagtatt taagaacaag atgttatatg attatgtttt
     4261 taaaaatgaa aaaagtaaaa atgattttct aaaaatggct gaatcatggc taccacagag
     4321 tgaaccaata gtaataaata atgatgatga cgcattgaat gctgctgctt atttttctgt
     4381 aaaaaaagcg aaaataaaaa cagtaaacga tactgatttt aaagagtata ataaggttta
     4441 tattcttggg cacggtagtc ctggttctca tcaattaggc cttggttcgg aacttattga
     4501 tgtacaaaca atcatttcaa gaatgaaaga ctgtggtatt ctaaatgtga aagatatccg
     4561 ttttacttca tgcggctccg ctgataaagt ggctcctaaa aattttaaca atgcccctgc
     4621 tgaaagtctt tcttgtatcc ttaactctct gccttttttt aaggaaaaag aatctttgct
     4681 agagcagata aaaaaacacc ttgaaaacga tgagtcattg agtgatggtc taaaaatatc
     4741 cggctatcat ggatatggag ttcactatgg tcaagagctt tttccctact cacattatcg
     4801 ttcaacttca attcctgctg atccggagca tacagtaaaa agaagctctc agaaaaagac
     4861 ttttattatt aataaagaac tggattagta taaaattttt aacctatagt taattgtttg
     4921 catttaagat gaagttattt tacctttagt agaaaagtgc ttccctatga gggaaaatca
     4981 caaatcatca taatacagag acaaaacgat acgaacaaat agataaaaaa catcattctt
     5041 ttaccacatt atttttcgtg aatatgaaaa ataatgttat tactttaata taagactatt
     5101 ttttgttttt ccatcactct gttcaaattt ttccgcatga cttgtatttt ttgtaacaca
     5161 gctcgttttt tacagctgac caaaatcatc aattaattat gctaaggaaa taaattatga
     5221 aaaccaaaaa ctttcttctt ttttgtattg ctacaaatat gatttttatc ccctcagcaa
     5281 atgctctgaa ggcagaaggt tttctcactc aacaaacttc accagacagt ttgtcaatac
     5341 ttccgccgcc tccggcagag gattcagtag tatttcaggc tgacaaagct cattatgaat
     5401 tcggccgctc gctccgggat gctaatcgtg tacgtctcgc tagcgaagat gcatactacg
     5461 agaattttgg tcttgcattt tcagatgctt atggcatgga tatttcaagg gaaaataccc
     5521 caatcttata tcagttgtta acacaagtac tacaggatag ccatgattac gccgtgcgta
     5581 acgccaaaga atattataaa agagttcgtc cattcgttat ttataaagac gcaacctgta
     5641 cacctgataa agatgagaaa atggctatca ctggctctta tccctctggt catgcatcct
     5701 ttggttgggc agtagcactg atacttgcgg agattaatcc tcaacgtaaa gcggaaatac
     5761 ttcgacgtgg atatgagttt ggagaaagtc gggtcatctg cggtgcgcat tggcaaagcg
     5821 atgtagaggc tgggcgttta atgggagcat cggttgttgc agtacttcat aatacacctg
     5881 aatttaccaa aagccttagc gaagccaaaa aagagtttga agaattaaat actcctacca
     5941 atgaactgac cccataaagc tggacagcct gtatcaggct atggagggcc catagacaaa
     6001 tctaccctat atgagcatag gaggagtcta tgggcacacc acgttttacc cttgaattta
     6061 agggattact ggaaaggctg ggacatatcc ctccggcaga agcagaaaaa gcttattatg
     6121 ctgccatcgg aaacgatgat ctggcaacct gagttcacag ataaaacatt ctctaggaaa
     6181 ctttattgtt aatctcttca taattagatg ctttaatcgc atcctccaaa gatatgtctc
     6241 tgagttttat ttttttgaag ttatctgtac ttagcattct tggtatatga tactcaagct
     6301 gaaacacaaa gtttataatt ctatccagac taacaggatc aatgtttttg ttataacaaa
     6361 actctcggaa tccttgatct ttactctttc ttataaaatc aataagatgc aacccaattc
     6421 caagcctcat
//
LOCUS       DBKEKI010000194         6358 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.196, whole genome shotgun sequence.
ACCESSION   DBKEKI010000194 DBKEKI010000000
VERSION     DBKEKI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.196"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(21..1340)
                     /gene="potE"
                     /locus_tag="ACUQPX_003949"
     CDS             complement(21..1340)
                     /gene="potE"
                     /locus_tag="ACUQPX_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HHV9058443.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSTTLYVDSWNPHHAPFFSTVGSSIAMTLWAFLGLESACANTDVVEN
                     SERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(1337..3524)
                     /gene="speF"
                     /locus_tag="ACUQPX_003950"
                     /pseudo
     CDS             complement(1337..3524)
                     /gene="speF"
                     /locus_tag="ACUQPX_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            complement(3835..3939)
                     /gene="speFL"
                     /locus_tag="ACUQPX_003951"
     CDS             complement(3835..3939)
                     /gene="speFL"
                     /locus_tag="ACUQPX_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HHV9058444.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            complement(3918..4052)
                     /locus_tag="ACUQPX_003952"
     CDS             complement(3918..4052)
                     /locus_tag="ACUQPX_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HHV9058445.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA
                     "
     gene            complement(4214..4891)
                     /gene="kdpE"
                     /locus_tag="ACUQPX_003953"
     CDS             complement(4214..4891)
                     /gene="kdpE"
                     /locus_tag="ACUQPX_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HHV9058446.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(4888..>6358)
                     /locus_tag="ACUQPX_003954"
     CDS             complement(4888..>6358)
                     /locus_tag="ACUQPX_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HHV9058447.1"
                     /translation="AALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSV
                     VATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARY
                     REQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQ
                     GMTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLM
                     IPEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTV
                     LFGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWL
                     TLEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQA
                     EIGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAI
                     VDVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
BASE COUNT         1658 a         1673 c         1535 g         1492 t
ORIGIN      
        1 ccctttttta ctttgctttt ttaaccgtgt ttatttttca gttcaaagcg tggtgatacc
       61 agaccataca gtgtccaacc aaggaaggtc acgatggaac cgtacagcat ggcttcttcc
      121 ccggatgagt acagcgcata aaagctatac atcgcgccaa caaaagcaac aaagtttgca
      181 actttcgctt ttgagggggg cacattagcg accttctgaa taatgactaa tgccgccatt
      241 gacagaatat acggaatgat attggtcacc acggccaggt taaccagcac gttgaactga
      301 ctgtttagcg acgggctaat cgtcatcagc gccaacccgc tctggataat cacaatggtc
      361 aacattccct gcaccggtgc atccactttg gttacacggg agaaaatttt agggaagtag
      421 ccttcatcag atgaagattt aaacacctgg gcaatggtga actgccagcc aagcagcgaa
      481 ccgcagcagg acatcaccat cagcgccata atgactttac ccacttctgg tgtgaacatc
      541 tgtgcgaagg ccaggccaaa tggtgccgtt gaatttgcca gctccatatt aggcacaatc
      601 cctgcaatca cgttggtgga gacgatataa atcaccgccg cacctaacgt accgccgagt
      661 accgcgattg gcacattacg ttccgagttt tccactacat cagtattcgc acacgcagac
      721 tccagaccaa gaaaagccca cagcgtcata gcgatggaag aacctactgt actgaagaac
      781 ggtgcatgat gcggattcca ggaatcaacg taaagcgtcg tgctaaacca gaaccagccg
      841 ataatgcaca ggcctaccac cggaataatg accccccaca cggtaatgct actgatttgc
      901 ccggtaatgc gcgcaccacc aaagttagcc acggtacaaa tccacagcac cccgatggtc
      961 gcaagaccaa tctgcactgg cgacaaactc gcgccgagca gttcggtgcc gtaaccaacc
     1021 gccgaaatag caatcgcgac gttagcaatc agcagtgaga cgccgtaggt atagttcgcc
     1081 ataaagttac cggatttacc gaaagcatac tcggcataac cgcccatacc acctgattta
     1141 cggctgaaca taccgcattt tgcgaatgcc catgccagtg ccattgagcc tacggctgtc
     1201 accagccagg agataattga aattgtccca acttcggcaa gctttgtcgg cagcatgatg
     1261 ataccggagc ccatcatgtt gaccatcgtc agtatggtta actgaacgac gcccattttg
     1321 ttcgatttag cctgactcat aatttttccc ctttcaacag ggtgctttgc gcatcacgag
     1381 gcttgatgac atagcaccaa acttgcttac gaccatcatg ttcttcgata tagacaccct
     1441 gcagctccgg tgcaaaacct ggcagcaggt tgatcccttc ttccagagcg ctgaagtaac
     1501 gcaaaacagc accaccccag atttcacccg gaacaacaca cagcacaccc ggaggataag
     1561 gaagcgcccc ttcggcagcg atacgacctt cggcatccgg cagacgaacc agctccactt
     1621 caccgcgtaa ataggcgtag ttggcttctt gcggattcat gctgacgtgt gggaagtgcg
     1681 ctttgcggaa catctctttt tgcagttgtt tcacgttgtg gcgggcatac aaatcatgca
     1741 tttcctggca caactgacgc agggtataac cggcgtagcg ctcttcatgc tgtttgtaga
     1801 tggaaggcag cacttctgct aatggcgcgt cggactcaag cagtttttcg aagcgtacca
     1861 gcagggcaac aagttgctga agtttggcca tatcttctgc cggagtcagc aggaagagga
     1921 tggagttaag atcgcatttt tccggcacta cgcaggaagt tagcaagaat cgtcgcgggt
     1981 acaccgaacg cttcatattc gccgttacgt gcatcaatac ctggggttgt cagcaacagt
     2041 ttgcaaggat cgacaaagta ttgattctct gcatagcctt caaaagaatg ccagtgttcc
     2101 cctggtacaa actggaagaa gcgcagatca accgcaattt gcgctgtttc atacgactgc
     2161 cagggtttac catccaccag ttcaggtacg aacggacgaa tatgctgaca gttatcgagg
     2221 atcagtttgc gggcattaat gccatttacc acacaatcca tccacatatt acgaccgctg
     2281 acaccttcat gcattttggc gttgatatcc agtgcggcaa acagcggata gaacgggctg
     2341 gtggaggcgt gcatcataaa ggcgttgttc atgcgtttgt gcggtacata acgctgttgc
     2401 cctttgatgt ggctgtcttt tttatggatt tgtgaagttt gagaaaaacc agcctgctgt
     2461 ttatgtacgg attgcgtaac cagaataccc ggatcgttct cattaagatc cagcaacagc
     2521 ggcgaacagt cagccatcat cgggataaat tgctcatagc caacccatgc ggagtcaaac
     2581 agaatgtagt cacacagatg accaatttta tccaccacct ggcgggcgtt ataaatcgta
     2641 ccgtcgtagg tgcctaactg aatcacagcg aggcggaaag gacgagcctc ttttgcccgc
     2701 tgcggtgcga cttccgcgat cagctcacgc aggtaacttt cttcaaaaca gtgcgcatca
     2761 atgccaccga taaagccata cgggttacgt gccgtttcca gataaaccgg tgttgcacca
     2821 gcctgtagca acgctccgtg gtggttagat ttgtggttat tgcgatcaaa cagcaccaga
     2881 tcacccggtg ttagcagggc gtttaaaacc gctttgttag aagatgaagt gccatttaga
     2941 acgaagtatg ttttatcggc attaaacact tttgccgcat gttgctgtgc aatgcatggc
     3001 gcgccttcgt gaatcagcag atcgcccatt gctacgtctg cgttgcataa gtcggaacgg
     3061 aacagcgttt ctccgaagta ctccacgaac tgattccctg ccggatgacg acggaaaaac
     3121 tcacctccct gatggcccgg gcagtcaaaa gcgctattgc cctgattgac ataatcgacc
     3181 agtgcgcgga agaagggcgg gcgcagttgc gtttcataat ggctagcagc ggtttctaac
     3241 tgacgtccat aaaactcttt ccgtgattcg caatgctcga agacaccaga aatacgtggc
     3301 agatattctg ctggtacacg ttcttcattt tccgttgcaa taaaaacagg aatgccatag
     3361 ccagtagcgt ctattttatc gagttttcca cgttcaacat cattgactga taaaataatg
     3421 gcggcaacgt caatataatt actttcgtca atattcacgc atgcacgatg agtggaaaaa
     3481 caatccgggc aagaacggtt aacggcaatt tttaattctg acattttatt catctcttta
     3541 tttcaggtaa tagcaggtcc tcaatttctc aaaggagaaa tcgatcacgc cggaaaaaaa
     3601 gcaaagctcc accgctgata aataaatcag tagtttgctt tttgcagatg caataaccca
     3661 gaccagcccg gtaactggct atactcgtca tacctcaagt tgcaagtgct gcgactcgaa
     3721 ttatttagag tttaaaaggg ttagcagtat atgagcaccg aggaatacag gtattaacct
     3781 gtaatgctta aagtcaggga gacctgtacg ttggggtgcc ctttagtcag aagactagcg
     3841 ggcattaaag aaatgaaagt caaaactatt acgatgggca aacttcatga tatgggttgt
     3901 ccgccttata tgaggcatta agcggttatt attttccatt tacctttacc gcttgctgtg
     3961 tcagttgaag gtagagttat tctatccatg aaatggctat aatctgtgaa gaagatcaac
     4021 aaaaaacgat cataccccag ataataaatc attaattatg aatatcgcca tattagatgc
     4081 tgttttgtta cttaaatgtt gtaatttcgt gttttatatt tgcgttaaga aattaaacct
     4141 gccaaataat aatattcggc gcaggtttaa taataaatta atcactattt aagcgaattt
     4201 attgaataac aattcaaagc ataaaccgat acccaatacc ggtttcagta atgaagtggc
     4261 gaggccgggc cggatcctgt tccagttttt gtcgcagatg tcccatataa atacgcaaat
     4321 agtgactgtg ttcgaccgcg tttggccccc acacctggtt aaggagctgg cgctgggtga
     4381 gtacttttcc ggcattgttg agcagcaccg ccagcaggcg gaactcaatt ggtgtgagat
     4441 gcacctcttc ctcaccccgg tgaatcacgc gggcggctaa atcgacggta acatcggaaa
     4501 atttcaccag cggatcgggc gcggcggtgg cggagtggcg gcgtaaagcg acgcgcagac
     4561 gggcctgcaa ttcgccaatg ccaaacggct tactcagata atcatccgct ccggcatcca
     4621 gcgcggcgat cttgtcgctc tcttcgctgc gtgcggaaag cacaatcacc ggcaccgggc
     4681 tccactggcg caggtcgcgg ataaactcaa tcccatcacc atcgggcagg ccgagatcga
     4741 gaataatcaa atctggctta cgggttgccg cttccagcaa gccgcgttgc agagtttcgg
     4801 cctcaaagac gcgcatcccg tcgccctcca gcgccgtgcg cagaaagcga cgaatagcct
     4861 gttcatcttc aacaatcaga acgtttgtca catatcctca tgaaagtctt caagttcagg
     4921 agcggtttgc tggggaagtg taacacgaaa acaggcacca ccttccggtc ggttgaacgc
     4981 ggtaatggtg ccgccgtgta catccacaat cgcccgacaa attgccaacc caagcccgac
     5041 acccggcacc gccgactctt tattcccgcg agcaaactta tcaaatatcg tctgctcctg
     5101 gcctggcgga agaccggggc cgttatccca gacatccagt tgtagatttt cgccctcaac
     5161 gtgggcatcg ataccaattt cggcctgcgc acccgcatat ttcaccgcgt tctccagcag
     5221 attaatcagc acccgttcaa agagtggccc gtcaacgtgg attaaggtca gcggttctgg
     5281 cagagaaaga ttgatgggcg acgataaacc cggttccagc atttgcagcg cgctgccgac
     5341 tacttcttcc agcgttaacc actctttctt caaattaaag ccgccggact gaattcgcgc
     5401 catatccagt agattattca ccagtcgggt agtgttcagc acatgctgac gaatctcgct
     5461 ggcctggcgg gcgtggggtg atccttcgct tgccagatcg agcgttaaga tttctgcctg
     5521 accaaacagc accgtaagcg gcgtgcgtaa atcatgcgaa agcgctgcca gcagggcgtt
     5581 gcggatctgt tcacgttcgc ttgccatccg cgcctgttct tcgctggcgg ttagcgtcag
     5641 ccgctcaagg gcattggcga ctaacagcgt aaacgtctcc agcaggcgct gctgttccgg
     5701 gatcatcaac tggcgcagat tccccggctc caccaccacc agcccgtagg ttttctcgcc
     5761 gctttttagc ggcaaaatct ggtacggtac accgggtaac gtgtcggtgc ccgcgcccgc
     5821 aggcaggcct ttatcaaaac tccactgcgc gatggcatcg tcccatggcg tcattccttg
     5881 cggatgtgtt aacggctgca atttaccgtt gtcatcgggc aacaacacct gactgcgggc
     5941 atgaaacgtg gaggcaataa attgttcgct ggtggcggcg atgtcctgcg gactgcggcc
     6001 caccgccaga gctttcgaca tttcatataa gtgccgtgtg cgttgctcgc ggtaacgggc
     6061 tacccgcgcc tgataacgca cgccagcagt aaggttcccg atcaccagcc cgacggttaa
     6121 catcaccgcg aaggtcagca gatattgcac atcagagacg gcgagcgtgc cgcgtggggc
     6181 gataaaaaag agatcgaaac tcaccacatt aatgacggtg gcaaccaccg aaggccagcg
     6241 tccataaaat agcgccacca ccaccacgcc aagcagatac agcatcacca ggttggcggc
     6301 atcaaacgcc atcagccact gcatggcaat taaggtgata acggcgcata acgcggcg
//
LOCUS       DBKEKI010000195         6302 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.197, whole genome shotgun sequence.
ACCESSION   DBKEKI010000195 DBKEKI010000000
VERSION     DBKEKI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.197"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(173..1012)
                     /locus_tag="ACUQPX_003955"
     CDS             complement(173..1012)
                     /locus_tag="ACUQPX_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HHV9058448.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGISTPKVTRAHNRAEAVFLC
                     VMHSHIQIMVRRAGQPQGWPGSRVTGSANPVRLTTHEISTSAGELINLSLEDVIMATT
                     LSHPDVTIENGRAVTTSISIAEFFGKQHHHVVQKIESLECSELFLTSNFSRVKFEHRG
                     NSYNAYQITKNGFVFLVMGFTGKKAATFKEAYIAEFDRMEAELRQNNTPPADKMIPGD
                     GRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQLL
                     SLK"
     gene            complement(1434..1586)
                     /locus_tag="ACUQPX_003956"
     CDS             complement(1434..1586)
                     /locus_tag="ACUQPX_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HHV9058449.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LAKNTS"
     gene            complement(1780..2187)
                     /locus_tag="ACUQPX_003957"
     CDS             complement(1780..2187)
                     /locus_tag="ACUQPX_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9058450.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNDLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPDPPVALNSALDLSEDELEMLRLYRALPKS
                     EQQAQISELRARVENFNRLFTELLEARKRNKHQ"
     gene            2265..2492
                     /locus_tag="ACUQPX_003958"
     CDS             2265..2492
                     /locus_tag="ACUQPX_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HHV9058451.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWGELVPEGRAMRLQK
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            2476..2898
                     /locus_tag="ACUQPX_003959"
     CDS             2476..2898
                     /locus_tag="ACUQPX_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HHV9058452.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGASDDSVVVH"
     gene            2989..3744
                     /locus_tag="ACUQPX_003960"
     CDS             2989..3744
                     /locus_tag="ACUQPX_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9058453.1"
                     /translation="MSMKLMAKAMEIKVGNPLRKLVLIKLADNASDQGECWPSYQYIA
                     DQCEISKRSVINHIAALCESGLVKKITRKGEKGNTSNIYLLHLDGAENLPGNSANNSL
                     HCASDASGSAGVAPVASAGVAPRTSHSFEPVNEPDVGGSADADPQVSHRAKNKIDWQR
                     VLDSYHEILPEMPSVKILTDTRKKNLRTFWQKFGFDQQRWESYLRYVAENCRWMLEDR
                     PNGSGGFWKRKNLDYLITERCYVAVKEARANDR"
     gene            3734..5137
                     /locus_tag="ACUQPX_003961"
     CDS             3734..5137
                     /locus_tag="ACUQPX_003961"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HHV9058454.1"
                     /translation="MTDNTFPVPYRVDAEQAVLGCLMVNTDTERAGLVYSMLKPESFY
                     VAAHRVIFREIRGLFRAGKPTDLLSLANVIEAKKLDAETGGFAYLAEISRSATLSAMV
                     HYAGIVREKSILRYAVEKLHACIGIMNQPTDTSATERLGAVQQVIGTVAEYARTGKTG
                     GLRPVSDVVNDWIDDLERRFSSPENAAGLTLGISSLDRLMAPKHVLRGSLVVIGARPK
                     MGKSASLNRIVTHFALNHRLPTLVFTLEMTDRSLVERMVAQEARVNSEIFYVGASDDS
                     DMARAMAKAAELAESNLMIDSTPGVTLAHVIAECRKVKRQRGVVGLVAIDYLTLMKAE
                     AAERRDIAFGDITTGLKNLAKELDCVVLLLTQLNRKLEDRADKRPTPSDSRDTGQIEQ
                     DCDVWIGLYRDAVYNRNADSQLMELILRLNREGATGTAYALMDNGSVTDVSAEDAARR
                     LRLGGPARKPYAQTEDF"
     gene            5137..5298
                     /locus_tag="ACUQPX_003962"
     CDS             5137..5298
                     /locus_tag="ACUQPX_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058455.1"
                     /translation="MQSLCDVLEKTLLQFGRGETAEQKTRNQKNLVHLRKMIDAARLA
                     EKLEKQNRR"
     gene            5304..6065
                     /locus_tag="ACUQPX_003963"
     CDS             5304..6065
                     /locus_tag="ACUQPX_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HHV9058456.1"
                     /translation="METVFDALKAMGKATSTELAARLDISREEVLNELWELKKAGFVD
                     KSAYTRRVADNNVQQEQPAQAELPEETTTATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQGGKFRYCIPGDNLPAEPKAASVAETDGKAFPQ
                     PAGVALPVQEAATQEDIKTETVADIVQSLPLFTETRADDLVLPSLHMANRELRRAKSH
                     VQKWECVCAALRELNKHRDIVRQITDISRRVASEK"
     gene            6081..>6302
                     /locus_tag="ACUQPX_003964"
     CDS             6081..>6302
                     /locus_tag="ACUQPX_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HHV9058457.1"
                     /translation="MAKVFTPEEREEVKARIVELVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSDCGVFPSEQAYRVWR"
BASE COUNT         1640 a         1412 c         1705 g         1545 t
ORIGIN      
        1 ctgtttattg cagagatggt aatcacattg tattaaatcc gggtcacctt tttgctggag
       61 aatttttctg attctccaca gccatttttt aatcccggta tgactgatgg tctgcctgag
      121 ggcaggtgta aaacaataat caagcatggg gttcatatcc atagctccat ttttatttta
      181 aggaaagcaa ctgctctacg gtcatgtttt taattgcgcc ccggtttaca agagtccagc
      241 cctgtttttc cagataaaac cggaaagtct ccagggtaca gaccagtgcg ccatcaggaa
      301 cggtttcggt gaattcgaca ttgccgaatt tgtcgaagtg aacaaccaga gtgcgaccat
      361 cacccggaat catcttgtca gcaggtgggg tgttattctg gcgcagttcg gcctccatgc
      421 ggtcgaactc agcaatgtag gcttccttga atgtggcggc ttttttgccc gtgaagccca
      481 tcaccaggaa aacgaagccg tttttggtga tttggtaggc gttataagaa ttgccgcggt
      541 gttcgaattt aacccgcgaa aagttgctgg ttaaaaatag ttcagaacat tcgagggatt
      601 ctattttttg aacaacatgg tgatgctgct tgccaaaaaa ctcagcaatc gaaatagaag
      661 tagtgacggc gcgaccattt tcgatggtta cgtcagggtg agaaagggta gtagccataa
      721 tgacatcctc aagtgataag ttaattaact caccagcaga ggtgctaatc tcatgggtgg
      781 tgagacgcac agggttagca ctaccggtca ctcgagaacc cggccagcct tgcggctgcc
      841 ccgcacgcct caccataatt tgaatgtggc tgtgcattac gcataaaaaa accgcttcag
      901 cgcggttatg cgctcgagta actttcgggg tgctaatccc ggcacccgtt ttatgaggtg
      961 caggtgcact ataattccac ccgttctggt tttcaatagc tacattcaac attttctctt
     1021 tcctttcatc accgaagtga attttgtgat gcggtgcctg gtgcctccag gtgacgttaa
     1081 ccagttaaca attacagtcg gctttcccac ccaaaacaat aaggactaac atgactttta
     1141 actgtgccgc gtgcgcttag ccgcattcac cgcatcacaa aattcacttt aaaaagggcg
     1201 gacatcagcc agcaattaaa ccgatgccgc caactggtac ttcacacagc aatgtcgtta
     1261 tttacaaccg gaagcgcact cccaccattt aaatttcaca gacaagaccg actctttatg
     1321 aataccggaa atgcgccttc gtgttgtgcc cggttttatt tcaccacctc cgggctttgg
     1381 tggcctcggc tatacctcta cagcgagaat attgaattaa tccaataaat ggattagctg
     1441 gtatttttgg caagccagcg acgtgcgcca gcttcggttt taaacgtttt gcttttggta
     1501 tacgtcatgg cggtgaatgt gccgtcctga ttgggaaaca cgccacacac tagggattcg
     1561 ttgttgccaa gatcgatagt atccatgctg acctcatttc cccttaacgc cggggtggcg
     1621 gaactgttta ctgagagcac cgtgcggtgt cttgatagat gttaatttag ttttctcatg
     1681 gtaattggtc aagtattttt gatgagaaat ctcaatattt aatgcaaaat aaagccaata
     1741 cattgaaatg taaggcttta aaatttgtga aggggggggt tattgatgtt tgttacgttt
     1801 gcgagcttct agtagctcgg tgaataggcg attaaaattc tcaacgcggg cacggagttc
     1861 gctgatttgt gcttgctgct ctgattttgg aagtgcgcga tacaatcgca acatctccaa
     1921 ctcatcttcc gataagtcta aggcgctgtt gagtgcaact ggtggatctg gtgttttatc
     1981 ctcgtcacca aacagtatcc aagttggtga acattgcaat acctcagcca ggcgatgcaa
     2041 attttgcccg cgcggggctg tatggtcgct ttcccatagt gaaattgatg agccagatac
     2101 gccagcagct ttgcttaaat cgttttgact taaaccaacc tgtttgcgtc tttctctaat
     2161 tcgttgacct aaagttttct cgttcatatt tagatatctt aataaccctt gacttgagat
     2221 tccttgagtg attactattg agaaaactca actctggagg ggtgatgttt aaatcagacg
     2281 taattaattt ttatgggacg aaagccaaag tagcgaaagc tgctggtgtt gatccatctg
     2341 ctgtttctca atggggtgaa ctggttcccg aaggtcgcgc gatgcgccta caaaaagcat
     2401 ctggcgggga gcttcagtac gaccccaaag tttatgacga atatcgtaag gcaaaacgac
     2461 ctgggaaggt gattcatgaa aatcaggcat gagcacatcg aatcagtgct gttagcccta
     2521 gcagccgaaa aagggcaggc gtgggtcgct aacgcaatta ctgaagaata tctgcgccag
     2581 gggggcggcg aattgcccct ggtaccaggc aaggactgga acaatcagca gaatatctat
     2641 caccgttggt tgaaaggtga aacgaaagcg caaagggaaa aaattcagaa actgatccct
     2701 gcggttctgg caattcttcc gcgcgagctg cgtcaccgac tctgtatctt cgataccctg
     2761 gaacgccgtg cattactggc ggcgcaggaa gcgttgagta cggcaattga tgcgcatgat
     2821 gacgcagtcc aggccgttta ccgtaaagcg catttcagcg gtggtggggc gtccgacgat
     2881 tctgtcgttg tgcattgatg tttatgccga acctcctctg gttctgttga ttggggaatc
     2941 acagattata tccggaggaa ggttcggcac cagacgaggt ttctatatat gagcatgaaa
     3001 ctaatggcaa aagcaatgga aattaaagtg ggtaatccat tgcgtaagct ggttctgatt
     3061 aagctggctg ataatgccag cgatcagggc gagtgctggc ccagctacca gtatatcgct
     3121 gaccagtgcg agattagcaa gcgttctgtg attaatcata ttgcggcgct ttgtgagtcc
     3181 gggctggtaa aaaaaatcac ccggaaaggt gaaaaaggta atacaagtaa tatctatctc
     3241 cttcatctgg atggtgcaga aaatttaccg gggaatagtg caaataattc gctacattgt
     3301 gcatcagatg catcgggtag cgcaggagtt gcacctgttg ccagtgcagg agttgcaccc
     3361 agaaccagtc actcttttga accagtcaat gaacctgatg ttggtggatc tgctgacgca
     3421 gatccacagg tcagtcatcg cgcaaaaaac aaaatcgact ggcagagggt tctggacagc
     3481 tatcacgaaa tcctgcctga aatgccatca gtgaaaattc tgacggatac caggaaaaaa
     3541 aatctgcgga cgttctggca gaaattcggt tttgaccagc agcgatggga gtcctatctg
     3601 cgatacgttg ctgagaactg tcgctggatg ctggaagatc gtcccaatgg cagcggtggg
     3661 ttctggaagc gtaaaaacct ggattacctg atcaccgaac ggtgctatgt cgctgtcaaa
     3721 gaggcgcggg caaatgacag ataacacctt tccggtgcca taccgggtcg atgccgaaca
     3781 ggcagtgctg gggtgcctga tggtaaacac cgacactgag cgtgcggggc tggtgtattc
     3841 gatgctgaaa ccggagtcgt tttacgttgc ggcgcacagg gttattttcc gtgaaattcg
     3901 cggacttttc cgtgctggaa agcctacgga cctgctcagc cttgcgaacg tgatcgaggc
     3961 gaaaaaactg gatgctgaga ctggtggctt tgcgtacctt gctgaaatca gcaggagtgc
     4021 cacgctatcg gcgatggttc actacgccgg gattgtgcgg gaaaaatcca tcctgcggta
     4081 cgcggtggaa aaattacatg cgtgcatcgg gatcatgaat cagccgacag acacgagtgc
     4141 gacagaacga cttggcgcag ttcaacaggt cattggcacg gtggcagagt atgccagaac
     4201 aggaaaaaca ggcggtctgc gcccggtaag tgatgtggtt aacgactgga ttgatgatct
     4261 tgagcgtcgc ttttccagcc cggagaacgc agcgggtctg acgctgggga ttagctctct
     4321 ggatcggctt atggccccca aacatgtgct acgtggttcg ctggtagtga ttggtgcaag
     4381 gccgaaaatg ggtaaaagcg cctcgctgaa caggattgtg acgcattttg ctctgaatca
     4441 tcgtctgccc acactggtat tcaccctgga aatgacagat cgcagcctgg ttgagcgcat
     4501 ggttgcgcag gaggcgaggg taaattctga aattttttat gtcggtgcca gtgatgactc
     4561 ggatatggcg cgggcgatgg cgaaagccgc agaactggca gaatcaaacc tgatgattga
     4621 cagcacacca ggggtaacgc tggcccatgt gatcgctgaa tgccggaagg ttaagcgtca
     4681 gcgtggcgtt gttggccttg tggcgatcga ttacctgacg ctgatgaagg cggaagccgc
     4741 ggagcgtcgt gatattgctt tcggggatat cacaacgggc ttaaaaaatc tcgctaaaga
     4801 gctggactgc gtggttttac ttctgacgca acttaaccga aaactcgagg acagggcaga
     4861 taaacgcccc acaccaagcg acagtcgcga caccgggcag attgaacagg attgcgatgt
     4921 gtggattggg ttgtaccggg atgctgtcta caaccgtaac gcagactctc agcttatgga
     4981 gctgattctc cgtctgaaca gggagggagc aacaggaaca gcatacgccc tgatggataa
     5041 cggttcagtg actgatgtca gcgcagagga tgctgccaga cgtctgagac ttggtggtcc
     5101 ggcacgtaaa ccctacgcac agacggagga tttctgatgc agtcactttg tgatgttctg
     5161 gaaaaaacgc tgcttcagtt cgggcggggg gaaacggcgg agcagaaaac acgaaaccag
     5221 aagaatctgg ttcatctgag aaaaatgatt gatgcagcca ggctggctga aaagctggaa
     5281 aaacaaaacc ggaggtaatt ttaatggaaa ctgtttttga cgcactgaaa gcaatgggaa
     5341 aagccacatc cacagaactt gctgcgcgac ttgatatcag tcgtgaagaa gtgctgaacg
     5401 aactatggga actgaaaaag gctggttttg ttgataaaag cgcgtacacc cggcgtgtgg
     5461 ctgataacaa cgttcagcag gaacagccag cgcaggcaga actgccggaa gaaaccacca
     5521 cagcaacagt agcgaaaatc tcagagtgcg atttaaccgc gacgattgaa caacgcggac
     5581 cacaaacggc tgatgagctg gctacattgt ttggtaccac atcacgcaaa gtggcttcaa
     5641 cgctggcaat ggcaatcagc aaaggtcgtc tgattcgcgt aaatcagggc ggtaaatttc
     5701 gttactgcat accgggcgat aatttaccag cagagccgaa agcagcatcg gtagcggaaa
     5761 ctgatggtaa ggcctttcct cagcccgcag gtgttgcatt accagtacag gaggctgcaa
     5821 cacaggaaga tattaaaaca gaaacggtgg cggacattgt gcagtcgctg ccattgttca
     5881 ccgaaacgcg agcggatgac ctggttttac catcgctgca tatggcaaac cgcgaactgc
     5941 gtcgggcgaa aagtcatgtc cagaagtggg agtgtgtctg cgccgcgctg cgggagctga
     6001 acaagcaccg ggatattgtt cgacagatta ctgatatttc tcgccgtgtt gcatcggaaa
     6061 agtgattgcc ggaggcacct atggcaaaag tatttacacc agaagagcgg gaagaagtga
     6121 aggcgcgcat tgtggaactc gtgcgcctga gcggacgaga aacttttcga caactggcag
     6181 ataaaacggg tgtcagtaag accgctattc gtcgtttatc tggtgcgctt gcggccagtg
     6241 gtgatgtctg gctctctgat tgcggggtat ttccatcaga gcaggcgtat cgcgtatggc
     6301 gt
//
LOCUS       DBKEKI010000196         6253 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.198, whole genome shotgun sequence.
ACCESSION   DBKEKI010000196 DBKEKI010000000
VERSION     DBKEKI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.198"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..962
                     /locus_tag="ACUQPX_003965"
     CDS             <1..962
                     /locus_tag="ACUQPX_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HHV9058458.1"
                     /translation="GEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHR
                     AGYTANGVPANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEE
                     RLRKEIAELRAKIERVPFIDTFDLRYKNYEKRPDPSSQTVMFCLMDVSGSMDQSTKDM
                     AKRFYILLYLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDE
                     VVKERYNPAQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITHRAHQT
                     LWREYEHLQSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            1109..2584
                     /locus_tag="ACUQPX_003966"
     CDS             1109..2584
                     /locus_tag="ACUQPX_003966"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HHV9058459.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVVIHKPISDSTLIQTKSNNVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            2765..4251
                     /gene="dgcJ"
                     /locus_tag="ACUQPX_003967"
                     /pseudo
     CDS             2765..4251
                     /gene="dgcJ"
                     /locus_tag="ACUQPX_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
     gene            4294..4797
                     /gene="yeaK"
                     /locus_tag="ACUQPX_003968"
     CDS             4294..4797
                     /gene="yeaK"
                     /locus_tag="ACUQPX_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HHV9058460.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(4798..4902)
                     /gene="yoaI"
                     /locus_tag="ACUQPX_003969"
     CDS             complement(4798..4902)
                     /gene="yoaI"
                     /locus_tag="ACUQPX_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HHV9058461.1"
                     /translation="MNDQMFVETLIITSSFFTIAVVLVLSVLLIERTG"
     gene            5072..5518
                     /locus_tag="ACUQPX_003970"
     CDS             5072..5518
                     /locus_tag="ACUQPX_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HHV9058462.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPITSGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(5475..>6253)
                     /gene="nimR"
                     /locus_tag="ACUQPX_003971"
     CDS             complement(5475..>6253)
                     /gene="nimR"
                     /locus_tag="ACUQPX_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HHV9058463.1"
                     /translation="HEAAVAFCIHAGTDELTSPVHQHRKGQLILALHGAITCTVENAL
                     WMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTCCTLKISPLCRELILT
                     LANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKIRTMVEMMAKGPVEWG
                     SLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQGLVKGDTVQKVAHTLG
                     YDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
BASE COUNT         1648 a         1443 c         1384 g         1778 t
ORIGIN      
        1 atggtgaagg tcaggatgaa tttgtctttc agatttcgaa agatgagtat cttgatctgc
       61 tctttgaaga tttggcctta ccgaatctga aacaaaacca acaacgccag ctgaccgaat
      121 ataaaacgca tcgggcgggt tataccgcta acggcgttcc agccaatatc agcgttgtgc
      181 gttccttgca gaactcactg gcgcgacgca cagccatgac ggcaggcaag cggcgggaac
      241 ttcatgcact ggaagagaat ttggccatca tcagcaacag tgaacctgcg cagctgctgg
      301 aagaggaacg tctgcgcaaa gaaattgcag aattacgtgc caaaattgaa cgcgtccctt
      361 ttattgacac cttcgattta cgttacaaga actacgagaa gcggcccgat ccctccagcc
      421 agacagtgat gttttgcctg atggacgttt ccggttcaat ggatcaatcc actaaagata
      481 tggctaagcg tttttatatt ctgctgtatc tgttcctcag cagaacgtat aagaacgtgg
      541 aagtcgtata catccgccat catacccagg cgaaagaagt cgatgaacat gagtttttct
      601 actcgcagga aacaggcggc accattgttt ccagcgccct gaaactgatg gatgaggtag
      661 tgaaagagcg ttataacccg gcacagtgga atatttacgc tgcacaagca tcggacggcg
      721 ataactgggc cgatgactct ccgctttgcc atgaaatcct ggcgaaaaaa ttattacctg
      781 ttgttcgtta ttacagctat atcgaaatta cccatcgtgc acatcagaca ttgtggcgag
      841 aatatgagca tctgcaatct actttcgaca actttgcgat gcagcacatc cgcgaccagg
      901 atgatattta tccggtgttc cgtgaactgt ttcataaaca aaatgcaaca gctaaagact
      961 aaaactatca gccgggtcat tatcgcctgg ctgattttta gcttactgta aattatctca
     1021 tttattacat acattagctt acaatcgctt taaatatgac agcataacct ttacacaatt
     1081 tagttccaga aaacaatcat tcggaaaaat gattcagtca acacgtattt ccctggggtt
     1141 attctttaaa tattttttat cgttaacgaa aattgatctt ggtcaaaact atatatctct
     1201 gccatcaata aaatccagca ctcacattgc tctccttttt atggtttcta tgggtacaca
     1261 aaaattaaaa gctcaaagct tttttatttt cagtttattg ctgacgttaa ttttattttg
     1321 catcaccaca ttatataacg aaaagacgaa tgtaaaactc atcccacaaa tgaattatct
     1381 gatggtcgtt gtggctttgt ttttccttaa cgccatcatt tttcttttca tgttaatgaa
     1441 atatttcact aacaaacaaa ttctaccaac actcatttta agtcttgcat ttttaagtgg
     1501 ccttatctat ttagttgaaa ctattgttgt tatccataaa cctatcagcg acagtacact
     1561 gatccagaca aagtcgaata atgtttctat tttctatatt ttccgccaac tcagttttat
     1621 ttgtttaacc tcgttggcgc tcttttgtta tggtaaagac aacatccttg acaacaataa
     1681 gaaaaaaact ggcattctgt tgctggcgct gattcctttt ttagtttttc cccttctggc
     1741 acacaatctg agcagttata acgctgacta ttctttgtat gtcgtcgatt actgtcctga
     1801 caaccatact gcgacctggg gaatcaacta tacaaaaata ttggtttgtc tgtgggcatt
     1861 tttactgttc tttattatca tgcgtacacg attagccagc gaactatggc cattaatagc
     1921 attattatgt ctggcatcgc tatgctgcaa cttacttcta ctgactctgg atgagtataa
     1981 ttacaccatc tggtacatca gtcgcgggat tgaagtctcc agtaaactgt ttgttgtgtc
     2041 ttttctgatt tataacattt ttcaggaact gcaactctcc agcaaactgg cagttcatga
     2101 tgtgctgacc aatatttata atcggcgcta ctttttcaac agcgtagagt cattattgtc
     2161 gcgacctgtt gttaaggact tctgtgtcat gctggttgat attaatcagt tcaaacgcat
     2221 caatgcccaa tggggacatc gtgtgggtga taaagtgctg gtttcaattg tcgatattat
     2281 ccagcaaagc atccgccccg atgatatttt agcgcgactg gagggtgagg tgtttggctt
     2341 gctatttacc gaactcaata gtgcccaggc aaaaatcatt gcggaacgta tgcgtaaaaa
     2401 tgtcgaactc ctgaccggct tcagtaacag atatgatgtt cctgaacaaa tgaccatcag
     2461 tattggcacg gttttttcaa cgggtgacac gcgtaatatc tcgcttgtca tgacggaagc
     2521 agataaagcc ttacgcgaag cgaaaagcga ggggggcaac aaagtgatta ttcataatat
     2581 ttaagtgcaa aaatattcag agccatgctt tttgcgtggc ttttgcatac aatttattac
     2641 tattacccct aaatttcccc tccccagcgg cgcagagatg agtataatta gcgcccctgt
     2701 gccaggccgc aatcgaactt tatctggttt tctcgtttca ctaaccgaag gagtgccatt
     2761 tatcatgaaa ttgcaccata gaatgctccg gcattttatc gccgcaagtg tcattgtgct
     2821 gacatcttcc ttatttttga acttgtcgcc agcgacagag caatgagtgc ctatctgcgc
     2881 tatatcgtgc agaaagcaga ctcctccttt ctttatgata agtatcagaa tcagagtatt
     2941 gccgcgcatg tgatgcgcgc tctcgctgcg gagcagtcgg aagtgtcgcc agaacagcgg
     3001 cgcgccatct gcgaggcttt tgagtctgcc aataataccc atggcttaaa cctgactgcc
     3061 cataaatacc cgggcttacg cggcacacta caaaccgcat ccactgactg cgacacaatt
     3121 gtggaagctg cagcactatt acccgctttt gatcaggcag tggaaggcaa ccgccaccag
     3181 gatgattacg gttcaggtct tgggatggcc gaagagaaat ttcactatta tctcgatctc
     3241 aatgaccgct atgtctattt ttatgagccg gttaatgttg aatactttgc gatgaataac
     3301 tggtccttcc tgcagtcagg aagtattggc atcgatcgca aagatattga aaaggtattt
     3361 accgggcgta ccgtattgtc gagcatttac caggatcagc gtactaaaca gaacgtgatg
     3421 agtttgctga cgccggtata tgtcgcaggg cagctaaaag ggattgtgct gctggatatt
     3481 aacaaaaaca atttgcggaa tatcttttac actcatgacc gccctctcct ctggcgtttt
     3541 ctcaatgtca cgctaaccga taccgattcg gggcgcgaca ttatcatcaa ccagagcgaa
     3601 gataatctgt tccagtatgt cagttacgtc catgacttac cgggcggcat tcgtgtctcg
     3661 ttatccattg atattcttta ctttatcacg tcttcgtgga aaagcgttct gttctggatt
     3721 ttgacggcgt taattttgct gaatatggtc cggatgcact tccgtttata ccaaaatgtg
     3781 tcgcgagaaa atattagtga tgcgatgact ggactgtata atcgcaaaat tttaacccct
     3841 gaactggagc agcggttgca gaaactggtg caatccggtt cttcggtgat gtttattgca
     3901 attgacatgg acaagttaaa gcaaataaat gacaccctcg gtcatcagga gggggattta
     3961 gcgattacgt tattagctca ggcgattaaa caatcgattc gtaaaagtga ttatgccatc
     4021 cgactcggtg gcgatgaatt ctgcatcatt cttgtcgatt cgacgccgca aattgcagca
     4081 caactgcctg aacgtatcga aaaacgtctg caacatatcg cgccgcagaa agagatcggc
     4141 ttctcttccg gtttttatgc gatgaaagaa aacgatacgt tacatgatgc gtataaagct
     4201 tccgatgagc gtttatatgt caataagcag aacaaaaaca gccgttcatg ataaccttct
     4261 gtggttgttt gcttgtaatc tcaggagcgt gaaatgactg aaatggctaa aggaagcgtg
     4321 acgcatcagc agttaatcgc tttattatca caagaaggtg ctaacttccg cgtcgttacc
     4381 catgaagcgg taggtaaatg tgaagcggtg tcagaaattc gtggcaccgc tctaggccag
     4441 ggtgctaagg cactggtatg taaagtcaaa ggcaatggcg taaatcaaca tgttctggca
     4501 atcctcgcag ccgatcaaca ggccgatctg agccaactcg ccagccatat aggcggatta
     4561 cgcgcttcgt tggccagccc ggcggaagtt gatgaactca caggctgtgt cttcggcgcg
     4621 atcccccctt tcagcttcca tccaaaactc aaactggttg ccgaccctct actcttcgaa
     4681 cgatttgatg aaatcgcctt caatgcaggg atgctggata aatccgttat tttgaaaacc
     4741 gcggattatt tgcgcattgc gcaaccagaa ctggtcaatt tccgccgcac tgcgtagcta
     4801 gcctgtccgt tcaataagca gaacggacaa aaccagcaca accgcaatag taaaaaacga
     4861 tgatgtgata atcagtgtct cgacgaacat ttgatcgttc atagcatgcc cccgggagat
     4921 agctgttttc acgttattgt tagcgtgcac aaatggcagt ttgatgacag ttcgcctgat
     4981 tttttatgca aaaaacgtaa agattttcta cttccttcct gcagcaagcg taaagtaagc
     5041 aggcttatta ttttttggca aggaaaccac gatgtttgat gtcactttgc tgatcctgct
     5101 cgggttagct gcgctgggct ttatcagtca taacaccact gtcgccgttt caattctggt
     5161 gttgatcatt gtccgcgtca caccgttaag cacctttttc ccgtggattg aaaaacaggg
     5221 gctaagtatc gggataatca ttctgacgat tggcgttatg gcccccatca ccagcgggac
     5281 gctaccgccc tcaaccttga ttcactcgtt tctgaactgg aaatcgctgg tggcaattgc
     5341 agtaggcgtt attgtctcct ggctgggtgg gcgcggcgtg acgttaatgg gcagccagcc
     5401 gcaactggtt gctgggctgc tggtgggcac tgttttaggc gtagcgctgt ttcgcggcgt
     5461 accggttggc ccgcttattg ccgcgggtct ggtttcgctg attgtgggga aacagtagtt
     5521 aatcccgcaa tatatcggcc tggcgtttgc cccaggcctt tcttaaacat agtaataaag
     5581 gccgttgttg aatcataccc cagcgtatga gcaactttct gtacagtatc gcctttcact
     5641 aatccctgaa gcgccataat cagctgtaac tgctgacgcc actggcgaaa actcaacccc
     5701 gtctctttca ctatcagtcg ggcaagattg cgttcactca tcgcaaaaaa gccagcccat
     5761 tgccccaatg acccccactc gacgggccct ttcgccatca tctccaccat cgtgcggatt
     5821 ttaggatgag aagaaacggg caggtgcagc tgttgttgtg gctgctgagg gagttcatca
     5881 aacaggacct gaattaatct gcgggtcatc ggctcagcac gctgagtcgt cgtcctgttt
     5941 gccagggtta aaatcaattc ccggcataag ggcgatattt tcaatgtgca acatgttgtc
     6001 ggcattgtca ctgcgctggg ttcgataaac agaaaacaga gttcagcgtt agctgtcact
     6061 tgattgctat gcttcacccc gcccggtatc caaaccgcat attgtggcgg caccatccac
     6121 aaagcatttt ccaccgtaca ggtaattgca ccatgtaatg ctagtatcag ctgtcctttg
     6181 cggtgctgat gtacaggact ggttaactca tccgttcctg cgtgaataca aaatgcgacc
     6241 gcggcttcat ggt
//
LOCUS       DBKEKI010000197         6179 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.199, whole genome shotgun sequence.
ACCESSION   DBKEKI010000197 DBKEKI010000000
VERSION     DBKEKI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.199"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..180
                     /locus_tag="ACUQPX_003972"
     CDS             <1..180
                     /locus_tag="ACUQPX_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HHV9058464.1"
                     /translation="IDELLRIKWWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQE
                     RDYKKFNLSEVFRGL"
     gene            complement(582..677)
                     /gene="yncL"
                     /locus_tag="ACUQPX_003973"
     CDS             complement(582..677)
                     /gene="yncL"
                     /locus_tag="ACUQPX_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HHV9058465.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            872..1045
                     /gene="ortT"
                     /locus_tag="ACUQPX_003974"
     CDS             872..1045
                     /gene="ortT"
                     /locus_tag="ACUQPX_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HHV9058466.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFTVALLFSLF"
     gene            1131..1364
                     /gene="ydcY"
                     /locus_tag="ACUQPX_003975"
     CDS             1131..1364
                     /gene="ydcY"
                     /locus_tag="ACUQPX_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HHV9058467.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1365..1814)
                     /locus_tag="ACUQPX_003976"
     CDS             complement(1365..1814)
                     /locus_tag="ACUQPX_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HHV9058468.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1811..2329)
                     /locus_tag="ACUQPX_003977"
     CDS             complement(1811..2329)
                     /locus_tag="ACUQPX_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HHV9058469.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            2510..3547
                     /gene="curA"
                     /locus_tag="ACUQPX_003978"
     CDS             2510..3547
                     /gene="curA"
                     /locus_tag="ACUQPX_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HHV9058470.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLAPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGEMLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMVQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            3745..4409
                     /gene="mcbR"
                     /locus_tag="ACUQPX_003979"
                     /pseudo
     CDS             3745..4409
                     /gene="mcbR"
                     /locus_tag="ACUQPX_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
     gene            complement(4692..5879)
                     /locus_tag="ACUQPX_003980"
     CDS             complement(4692..5879)
                     /locus_tag="ACUQPX_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HHV9058471.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPLVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQTDVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TARKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(5879..>6179)
                     /locus_tag="ACUQPX_003981"
     CDS             complement(5879..>6179)
                     /locus_tag="ACUQPX_003981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058472.1"
                     /translation="VVKLHEDDFFDEEDAEVLRFDSFDDAVECCADLNIPFFVDAGNK
                     KLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMYCPECGTVHWPDGVAPPF"
BASE COUNT         1682 a         1516 c         1482 g         1499 t
ORIGIN      
        1 attgatgaac tgttgagaat aaaatggtgg aattataact actcagatct tccggataat
       61 aacaagtgtg atgatataaa ttattttgtt gaagagatga atcgattgat atcaaatgga
      121 aatattcaag agagggacta taaaaaattc aatctttctg aggtattcag aggcctataa
      181 ttatctgttt tgcatgataa atgggcaaga taaccagggc ggggtttgca atttcaatgg
      241 ggcagcgtca tcaaggaggt tgcaaacatc ctgccatgca tatactttac gccatctttt
      301 tatatttacg ttattccttg aaagtataaa gcatttatag actcccgcgc gtataaagta
      361 ttcgtgatag aaaaatgtga aaacaagatt ccgtttgaat cagtaagctg tttgtataga
      421 cgacagtagg gggatgtttt ctactcattt caatgtagat gccagcaaaa agaagtaaat
      481 attaaggata atgttgcaaa cctggagagt atgagaagat cttgcgtact gtcagagagt
      541 tgatttctct gacagtacgt gctgcgtgca aaatcactac atcaaataaa ccagccaaat
      601 ctcatagaga taagagtaaa caaaccaaca gcagcaaaga catttatcag tactacagtt
      661 ctactggaaa cattcatttt tgccaccttt tatcattgcc cctatgtgta tagtacgggt
      721 taagaaaatc cgcaataata ctcattctga agatggataa gggcaagttg ctgtttgatg
      781 attttttctc aaactggtga aaaaacttga tgcacatcaa aaaatgacgc atatttgcgc
      841 gcgttttatt catatggctg gacgcccgta catgtctctc tatcaacata tgcttgtttt
      901 ttatgcggtt atggcagcaa tcgcatttct tatcacctgg tttctttctc acgataagaa
      961 acgcatccgt ttcttaagcg cctttctggt gggggccaca tggccgatga gtttcacggt
     1021 ggcgctgttg ttttcactgt tttaaatctt actcactggc ttgacggcac cacgcagact
     1081 tatatcattt ggattaatcg ataaatttca caagtggcta aggagaaagt atgtcgcatc
     1141 tggatgaagt catcgcgcgc gtggatgccg cgattgaaga gagcgtcatt gcccatatga
     1201 acgaattatt aatcgccctg agcgatgacg cggagttaag tcgggaagat cgctacaccc
     1261 agcagcagcg tttgcgcacc gctattgctc atcacggtcg caagcataaa gaagatatgg
     1321 aagcgcgcca cgaacagtta accaaaggcg gcaccatcct ctgattaaaa tgagcgtctg
     1381 gccaccaacc aggcgccaac cacaagtaaa attgcgccac atattgggcc gaacaacgcc
     1441 cgtagtggta cgccatgact gcggaagata tccagcacca gaccgccaat cagctggctg
     1501 gcgaccagta ccgcaatagt tgtcgccgct cccacattct gatatccgct gatactggcg
     1561 aagacaaaaa acgaacccag caagccaggt ataagtgtcc accagcgcac gctggacacc
     1621 agttcgccaa accccgccat gccttgttta aaccataaaa tagaaacaaa gagcacaatc
     1681 cccaccagtg agttcagcaa catggcgatg agaatggtag aggaggtctg ggtgatgcgc
     1741 accatcagcg tgttctggac taccagacca attccggcgg caatcaaaaa agcaagggtg
     1801 agcgactgat tcatccaatc gcgtccggtt cagtgcgctc gtcgagttgc aactgcataa
     1861 atgtcagatc cagccaacga ccaaatttag tgcctacctg cggcatttgt gcggtgacga
     1921 caaatcccag cgactggtgc agatgcagcg aagcctgatt ttgcgattcg atcccggcga
     1981 ccatgacatg cttcccgcaa tcccgcgctt catcaatcaa tcggcttaac aatttacgcc
     2041 ccagaccttt gccctgatga tcgggatgga cataaaccga atgttccacg gtatggcgaa
     2101 aaccatcgaa actacgccag tcgccaaacg aggcatatcc cgtaactacg ccatcttctt
     2161 cacttaccag caccggataa cctgctatgg tccgcgcttc aaaccaggca atgcggttat
     2221 ctgcatccac cgtttggtca ttccagatag ccgccgtata caacacggcg tggttataaa
     2281 tttccgcaat ggcagcacag tcggctttgc gggcaaaacg gatggacatg tctggcctca
     2341 ataaaataat gatgatgatg ttatcaatac tatcgaaaac agcccgaagc aacggattcc
     2401 tcgatgaaat tattgcgtgt tgcagagggg atgtgacggc tgcaaacaaa accaatcccg
     2461 tgagcctgct actattgatg gattcatgcc aaaagcacag gagagcatta tggggcaaca
     2521 aaagcagcgt aatcgacgtt gggttctggc ctcgcgtcca catggcgcac cggttccgga
     2581 gaatttccgt cttgaagaag atgatgtcgc cacaccgggt gaaggacagg tgttactgcg
     2641 cacagtttat ttgtccctgg ccccgtatat gcgtggacgt atgagcgatg agccatctta
     2701 ttcaccgccg gttgatattg gcggcgtgat ggtcggcggt acggtgagcc gtgtcgtgga
     2761 gtcgaatcat cctgattatc agcctggcga ctgggtgctg ggctacagtg gatggcagga
     2821 ctatgacata tccagtggtg atgacctggt gaaacttggc gatcatccgc aaaatccatc
     2881 gtggtcgctg ggtgtgctgg ggatgccagg ctttaccgct tatatggggc tgctggatat
     2941 cggtcagcct aaagagggcg aaatgttggt ggtagctgcg gcgacaggac cagtgggggc
     3001 gacggtgggg caaatcggca aacttaaagg ttgcagagtg gtgggggtag ccggtggcgc
     3061 ggaaaaatgc cgccatgcta ccgaggtgtt aggcttcgat gtttgccttg atcaccacgc
     3121 ggacgatttt gccgaacaac tggcgaaagc gtgcccaaaa ggtattgata tctattatga
     3181 aaacgtgggc ggtaaggtat ttgatgcagt gctaccatta cttaatacat ctgcgcgcat
     3241 tcccgtttgt ggcttagtga gcagctataa cgctacagag ctaccacccg gtccggatcg
     3301 tttacccctg ttgatggcaa cggtgctgaa aaaacgtatt cgcttgcaag ggtttattat
     3361 cgctcaggat tatggtcacc gcatccatga gtttcagaag gagatggtgc aatgggtgaa
     3421 agaggataaa atccactacc gcgaagatat tactgatggt ttagagaatg cgccacagac
     3481 gtttatcggc ctgctgaagg gtaaaaactt cggcaaagtg gtgatccgcg tcgcgggtga
     3541 tgattaaagt ttcaacggcg gcgtaagccg ccataaaaat ccaggcaaaa agcttatttt
     3601 cagctttaat taactaacca cgtcattgtt ctgctgctaa taacaacaaa ggtgggtggt
     3661 ttatgattat tcactttaat acaccaggtg aactccttct gccatgcagg cagggttgga
     3721 cagaaaacac gcgaaggaat taaaatgcct ggaacgggaa aaatgaaaca tgtcagtttg
     3781 actctgcagg ttgagaacga cctgaaacat cagcttagta ttggcgcatt aaaacctggc
     3841 gcacgcctga ttactaaaaa tctggcggag caattaggta tgagtattac acctgtgcgt
     3901 gaagcattat tacgtctggt ttcggtgaat gcgctttctg tcgcacctgc acaagcattt
     3961 acagtaccgg aagtgaggca aagtcagtta gacgaaatca atcggatccg ctacgaactg
     4021 gaattaatgg cagttgccct ggctgttgaa aacctctcct cgcaagacat agcagaactt
     4081 caggaattgc tcgggaaatt acagcaggcg caagaaaagg gcgacatgga acaaatcatt
     4141 aatgcgaaca ggctatttcg cttagcgatt tatcatcgct caaatatgcc catcctgtgt
     4201 gagatgattg agcaactgtg gatcaggatg ggacctgctt tacattatct ttatgaagcg
     4261 attaacccag cgtaattacg ggagcgcata gaaaactatc atctattact cgctgcgtta
     4321 aaagcaaaag acaaagaggg atgcaaacat tgtcttgctg aaattatgca acaaatattg
     4381 ctattttata ccagcaatac aatcgttaaa ttaaatgccc ggcaaatacc gggcatcaag
     4441 taattactca aatcgccacg caatattcat gccgacacca taatttcgtc cgggcgcagg
     4501 ttcatcgata ggaattaaaa ccccaaaaag attaaaaaaa caccacaaaa cggatgtttc
     4561 ttcaacacca cttttgctcc atatgaacgg aaccgacgat taaactggat ggctctgatt
     4621 gattcagggt atgaatggcg gttttttgct ccgtttccct caaaatggac gcaacttccc
     4681 ctctgcggct ctcagccgca ccaccgcatc cgggccagcg gctcatgcat caggacctgc
     4741 tctgccagac ggtagccccg cttcagcccc gtaaaacgca tctgactccc gcacagcacg
     4801 cacttcagcg ggtcaacctt cagtaacctc tgatacatcc ctctccaggt gatttgcatc
     4861 gccgtttttc tcgctgtctc cgttatgatg tacaccactt cttccagtaa ccgccgtttc
     4921 gccggactca aaaaaccgta gtacctcacc atacggaacc ccttatccgc cacatgccag
     4981 gagaaccttt ccatgaactc atctccactc atcaacaggt attcttcccg ttttgttcgg
     5041 tgactgttgt aacgcagacc gatttcatcc tgaccggcat aatgctccag acgactcatc
     5101 ggcaccggtg gctttttcag gtaagagcca aagtacaccg ccacatgggt ggcattatcc
     5161 atcacccggg atacgttgac attccagcca cggcggtaat gcgtgtccag gaagcgattc
     5221 cattcccgtt tactgcttcc ttctgctgcc agcgcatccg gcatcaccag gtcagggtat
     5281 ttccgtgaca gcaaccgtgt tatccggtag cgccacatgc tcatcacctt acgggcgtaa
     5341 aaatgaagat ttttccaggt gtggcccgac gtcacaccac cggcagttgt cgataaatgg
     5401 atatgcggat gccactgctg gtcacgcccc catgtgtgga tcaccgtgaa tatccccggc
     5461 tccacatctg tctgatggca gatttccagt atcacatccg ctgcaatgcg gctcatctct
     5521 gccagtaacc accggttgtg gaacaccagg gaccagtact ggcagggaag tgtgaacaca
     5581 atatgctgcc acgggcagtc ggggaccagg ctcagcagat actgtatcca ctgtgcgcca
     5641 gccttcaccc cgcagtgcgg gcaggagcgg cttttacacc ggaagcagac cttttttgta
     5701 tggcaacagt ccggtgatga acaacaccac tgcgtgtagc ccatcagtgt ggtcccgcag
     5761 gccatgattt ttgtcactga ttcaattacc aacggacgga ctgccccttc cggttgtttc
     5821 tccagccagt taagccagcg gtttccctgc tgaaaaatat cggcaaaacg gggaagcatc
     5881 agaagggcgg ggcgactccg tccggccagt gaaccgtgcc acactccggg cagtacatac
     5941 cgccggcgct gataccggca agaatggtcg caaattcccg ctccgtgcag cgggcgattt
     6001 ccggataccc ttcgtcatca acacgtacaa accagaagac cagctttttg tttcccgcat
     6061 ccacaaagaa cggaatattc aggtctgcgc agcattcaac ggcatcgtca aaactatcaa
     6121 agcgcagaac ttctgcgtct tcttcgtcaa aaaaatcatc ttcgtgaagc ttcacgaca
//
LOCUS       DBKEKI010000198         6166 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.200, whole genome shotgun sequence.
ACCESSION   DBKEKI010000198 DBKEKI010000000
VERSION     DBKEKI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.200"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1103)
                     /locus_tag="ACUQPX_003982"
     CDS             complement(<1..1103)
                     /locus_tag="ACUQPX_003982"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - gamma-aminobutyrate shunt
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HHV9058473.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICT"
     gene            complement(1465..4248)
                     /locus_tag="ACUQPX_003983"
     CDS             complement(1465..4248)
                     /locus_tag="ACUQPX_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HHV9058474.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(4305..>6166)
                     /locus_tag="ACUQPX_003984"
     CDS             complement(4305..>6166)
                     /locus_tag="ACUQPX_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HHV9058475.1"
                     /translation="KRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYF
                     SPDFKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDT
                     ALSKFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWA
                     KLRTTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRL
                     DWQKFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLG
                     IDNYTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGY
                     NRYYGGNILNMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIA
                     RANYVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDI
                     NPQIVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNM
                     DFTHQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTR
                     LSWTPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1544 a         1434 c         1431 g         1757 t
ORIGIN      
        1 gtacagatga actcatacgg ccccagtttg gcgatctcat ccgccagata ggcagcgacc
       61 tggtaggaag cattctgtac tttggtatag ccttcacgac caaggcgcag gaattcatag
      121 tactgtgcaa tcacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatctga
      181 ccaccgaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccagaaag
      361 ccaccgctgg cggcgtcgat gtgcatgtca atgtcgatac cggtgtcggc ctggaattta
      421 tccagtgcat cgtgcagcgg ttgcgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt attttcgtcg caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgcac cggaccgcac accaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatgtt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt gttaatggat aaatccatca acttgtggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggccaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctgaaatgc gacatcgtcg cgcatttcgt gcagcggaaa acgttttgat
     1021 tctgcgatag tggaaataga cttcgcacca aaacgtgaat cgagtagttc cgaccttaaa
     1081 tccgttactt gcttcttatc cattttaaac tccttaaaat gattggaacg cattaaaaaa
     1141 gtaggattta tcgataaagt aagcaagtgc aaaggactcg tgtttaaata acaaaatcct
     1201 aatgttattt atcgtgagat attgttggaa tgatattttt tcattaaaaa acaatgcaat
     1261 agagaattct tgggtggtgg caaggtgttt tatgttgtta tttttatgcg ctttctgtgc
     1321 ctcctgaatt atcacgtaaa aatcagacat taaaatatca ctattagtac ttggttatta
     1381 ttttgaacgc atttataaaa ttattacata aaaatagcga atattgctaa aatccccgcc
     1441 aacgatgtgt tgacggggct gttattattt tggcaataat actccggtat aagtatttac
     1501 cggatgagaa agatattgtt taacggcagt gttaacattc tctaccgtca tttgtttcaa
     1561 caattgctcc tgctcagtcc atgctgcagg atcgtcatat tgaataagac tatttataat
     1621 agtgttcgct aattgttgaa cgctacgctg ttggatatcg aggctgcgct gaacgttttg
     1681 ctggtattcg ttcagttctt gctcactgat ccccttagtc aaacgcttaa ccatcacttc
     1741 attcgctaac gttaacagtt catcatgtcg ttctggttga caagtaaaag ccagcaaatg
     1801 actgatatct ttagcctgag gatcaaccga gaggcgagaa gaaacgctgt atgccccaga
     1861 tgcctgttca cgaatattaa cgcgtagatc ttttgccagt gcgacgttaa aagcatcgag
     1921 cgccatacgc gtcgccagat taacaggtgt ccgggaatca taacgcttcc actgtgaaac
     1981 ctgtgccaca ggttcatttt gttcttttac agtaaccgat gcgttgtccg tcgcgcgagt
     2041 taatggttta cctgcggcta atggcgaatc agagtgtttg attgagccta agtaacgcgt
     2101 aattaacgcc acgagtttgt cttctgcgac attaccgaca atgacaaacg tgatatccgc
     2161 tggagatgaa aacaactggc gatcggcagc cagcgcatct gcggcagtaa actgtgcaat
     2221 ctgattttct tgcagtaatt tcgtgcggtc atcagcatag cgcgtctcat acatcagctg
     2281 ggcgaatttc tccgctggac gctggtcgag cgttttcaac gcctgaattt gagcattttg
     2341 tagcgatgcc caaatattgt cgttaatcgt gctgtgggtg attcgctggt taatcaactg
     2401 gaaaccaggt tcagggttat tagtccgcgc gctaacggag agcaacgtat tcatgccact
     2461 gactttactg ctcatggtta ccgagttttc cgcactccag cgtttcaggc tggaagagga
     2521 gagttcgcca acgccgcttc cgctaactgc tttatttgcc agcgcgataa gtgatttttg
     2581 ctgcgcaggg aaacttaaat cgcctttatt cgatacggca ataatttgta gcttttgctc
     2641 ttcacccgcg gattttgcca gaataacctt ggcaccattg gaaagtgtta atgatgtcag
     2701 attctcagcc agcgtttctt tgctgctaat ttccgcctgt gggtcagcgt ctactgttaa
     2761 cgataaattt ctgcctggga agatgtaagc cgccagcttt ttgttggcgt actctttttc
     2821 cagcgccagg atagccgcag gagacaatgc ttttttggcg gcaagctcat tgtttaccat
     2881 ttgctcccaa aatgcgtcct ggttctttct taactgctgc catttttccg ccagactttg
     2941 cacggtaatt tgctgccaca gacgtttcga aagttgatat gtctcttccg gcgacaagaa
     3001 cggcgtatta tttaatgagc tggatgccag gcgactggtc agcatacgta aatcacgttc
     3061 agcttgctga tcaaccgcat ttttcagcca ggtgaggcgg gtagatttga catcatcgag
     3121 ttcttcagca gaaaaaccat gctgatcaat ggttgccaac tctgccatta atgcattcgc
     3181 agcatcctgc atattatcgt ctcgtgcatt tacacggaaa aacagcgact gataatcggg
     3241 tgcaatttta acgctgcgcg cagtgccgcc agaaatagtc ttcaactcgc ccgactgtat
     3301 gcgttcctgc agacgttgat tgaacagctg aactaacatg ctccattcag cttgttcgac
     3361 aaagctttgc tcatcgttca cttgtaccat tggcaggcga taatagagtg cgatgccgtt
     3421 cacccggttt tctttatcat taatgatatt aaagcgcagg tggttttcgg cttttgtcgg
     3481 ccagacgcga ttttcagctg ctttgttagc cggaagctta cttaaattat cctttatcag
     3541 cgccagcgct tctttactgt cgatatcgcc gaccacgata aaggtcatat tatttggttg
     3601 ataccagcgt tgataaaatt ggcgcaattg tgccggtgtg accgtggcga cagtatccat
     3661 caggccgata ggttcacggt ctaaattacg ggtattagcc agcaggaaag ggcggcgcgc
     3721 ctgagaggtg cgccatttcg catcctgatg ggcacgccat tcctcagtaa ttacgccacg
     3781 ttcagcgtct acttcgagtt tttcaaaggt tgcggcatta ctccattcac tgaagattgc
     3841 catcacttgt tgcagatttt gtttctgcgt agtcggcaaa ctcacctgat acaccgtttc
     3901 gtcatagctg gtataggcat taacatcgcg accaaaacgc aggcccattg actcaaatgt
     3961 ttcgatgact ttattacccg gccatgtttt tgtgccgtta aacatcatat gttctacaaa
     4021 atgagccacg ccgcgctcat tgtcttcttc ctgcaatgaa ccggtatgaa tttgcagcca
     4081 taaatttacc tgatcctttg gatgagcatg cggataaatc atatatcgca agccattgtc
     4141 cagttgcccg gtaattaact tttcatcctg cggtaaggcg gcggcaatca gccgcccggg
     4201 gagcaacaga gttgccacta acgtcagtaa gaaacagagg tttctcataa ttatctccat
     4261 gcgaaaaccg ggcgaattta cccggttaag taaaatccga actattaaaa tttcatgctg
     4321 acatcaagcc agaaagtacg gccactggcg taggtcgcca caccggtgtt cgttgtatca
     4381 acagcggttt tgctatccag tacattgaga atatcggcac tgatcgtcag gttttgttgt
     4441 ttcagaaatt gtggcgtcca ggacaagcgg gtgtcccagg tcaggctgct atccagtttt
     4501 tcgtcaacat actgcttgta atctgaatat tcgctgatgt attgcgcatt tgtcttacca
     4561 aggataatgc gagctttacg cgcttcttgc caggccagcg tattcgccca cactaacccg
     4621 ctcggttgat gcgtgaaatc catgtttaag gagatcttta atgggttatt aaaatctgcc
     4681 actggaacgc tatcgtaaga gaccagatta ccgttataaa ccacctggtt atcaccggta
     4741 ttgctctcct cataaccatt gtttaacgac aagttgccct tgctcttgat atagctaaag
     4801 acaatttgcg ggttaatatc tacctggcgg atatgcaggg gttcggccag ttcaaaactg
     4861 aggttaaacg aatgcgtttt ggttttgcca tcgttgttat attcagtaat ggtggtttta
     4921 gtcgcgctgt cggtacgact gcttttgctg atttgatcat gcgcttcacg gtaaacatag
     4981 tttgcgcgtg caataacgtt cttatcgatt ttttgctgca atcccattgc cagttcatcg
     5041 ttataaggcg ttttcaaatt ctgataacgc gtcagggttt tattacctga taccgattcc
     5101 gtccagctat tgcggatatc acgtaatccc atattaagaa tattcccgcc atagtaacgg
     5161 ttataaccgg cggtaatcat tgaggtttga tcagcaaaaa tatcccattc cgtcataaag
     5221 cgcggggaga tattgtggtt tgacagatag ttgtcatagt cataacgcac accgggcatt
     5281 aacgacacat tccgccagct aatgtgatcc gccatataca gcgtgtagtt gtcaattccc
     5341 aggctgcctt tacctttatg gtaaatggta tggttagttt tctttcctgc agcattaatc
     5401 acataggatt cagactggtt atggcgttca gtccatgcat cggaatagat gtattccgcg
     5461 ccgaagtagg gttgatgcga aacatcaccc acggcgaatt tttgccagtc caggcgtgtt
     5521 ttgaaggtgt aattatctac agcctgggaa atgtgtccta atccgccacg ggtacaacgc
     5581 cctgtaatat caccatatgt acatgaaagt tcggtgtacc agatgtcatg atcgtgacgg
     5641 gtataatcac taatatgatc ccaaccaacg gtggtacgta gtttggccca tgcgagttgc
     5701 gtatccatat cccatgccag accataggat ttattaccca tttcgcgatc agactgcggg
     5761 aaggtgctgg tattataatc acggctggag ccggtatatt ttaaggttaa atcgtgggta
     5821 aagcggtcgc tggcaaacca ggtaaatttg ctcaatgcag tatcgataac gtttttatac
     5881 tgtgcccgac cggcgacaat gccgtcattc gaaacataat ccgcgcgggt gatatcagac
     5941 tggcggcgcg ataaaccggc ggtaacgcca aagttatcag ccagctcctg attaaacgac
     6001 aaggtataaa agttcttttt aaaatctgga gagaaatagg tacttcctga agaaccttga
     6061 ttaaatgcgc tcttgttatt ctcatcgata tgcgatgtta accagtccaa acgcgtagtg
     6121 cgataaccca atttcacctt gctatcatca gcgttgaagc gtttga
//
LOCUS       DBKEKI010000199         6123 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.201, whole genome shotgun sequence.
ACCESSION   DBKEKI010000199 DBKEKI010000000
VERSION     DBKEKI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.201"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..173
                     /locus_tag="ACUQPX_003985"
                     /pseudo
     CDS             1..173
                     /locus_tag="ACUQPX_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(490..828)
                     /locus_tag="ACUQPX_003986"
     CDS             complement(490..828)
                     /locus_tag="ACUQPX_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058476.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHYRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            750..1877
                     /gene="ltrA"
                     /locus_tag="ACUQPX_003987"
     CDS             750..1877
                     /gene="ltrA"
                     /locus_tag="ACUQPX_003987"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HHV9058477.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDATRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFKGERYRKCMPGASNAAM
                     RKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRL
                     LKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE"
     gene            1960..2139
                     /locus_tag="ACUQPX_003988"
                     /pseudo
     CDS             1960..2139
                     /locus_tag="ACUQPX_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2166..2651)
                     /locus_tag="ACUQPX_003989"
     CDS             complement(2166..2651)
                     /locus_tag="ACUQPX_003989"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HHV9058478.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(2907..3887)
                     /locus_tag="ACUQPX_003990"
     CDS             complement(2907..3887)
                     /locus_tag="ACUQPX_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HHV9058479.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3887..5086)
                     /locus_tag="ACUQPX_003991"
     CDS             complement(3887..5086)
                     /locus_tag="ACUQPX_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHV9058480.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5260..5394)
                     /locus_tag="ACUQPX_003992"
                     /pseudo
     CDS             complement(5260..5394)
                     /locus_tag="ACUQPX_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5408..5749)
                     /locus_tag="ACUQPX_003993"
                     /pseudo
     CDS             complement(5408..5749)
                     /locus_tag="ACUQPX_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5813..>6123)
                     /locus_tag="ACUQPX_003994"
     CDS             complement(5813..>6123)
                     /locus_tag="ACUQPX_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHV9058481.1"
                     /translation="LQRGVMAPRLLIIDEIGYLPFSQKEAKLFFQVIAKRYEKSAMIL
                     TSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE
                     "
BASE COUNT         1662 a         1406 c         1461 g         1594 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtggagg tgggccctcc ggtcagggtt tacaggagca
      241 ctgaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtggggttc gggaagctga acgaaagtaa actgtccgat
      361 gaagcatcga aatcttagat gatgtcaaaa tggggccgta cagccgtccc ctgataagcg
      421 cagaagacac ctgcttactg actgcgcggc atccggtgtg aaggcagcag gatcccgggc
      481 cagaccatct cagggaacgt gagaacctgt ttccggcacg tatcgggaaa caggggcgga
      541 cccattcgta gtagtgatga aagatctgta atgggtctgg agcgaaggga tggggatcac
      601 attcattgca gaagacacaa cccaaaaggg gatgacgtaa ctgatgcaac gaaaatcatt
      661 tgaaatacca aaagcactgg tctgggcatc atatctggat gtgcgtcgca acaagggagc
      721 tccccggatg cgacgggcag actttgaaaa tgttcgacca gcaacgtgat ggcaatctat
      781 acaagatctg gaaccggcta tgttcgggaa catggtttcc gccaccagtg ctggaaaaac
      841 ggatcccgaa gtcgaatggc aaggaacgca ttctggggat cccaacagta tccgatcgaa
      901 ttgctcaggg agcgataaaa cttttcatgg aagaaaagct tgacccgatt ttccatgctg
      961 attcatatgg ttaccgacca ggcaaatcag ctcatgacgc tctgaaacaa tgtgccatcc
     1021 ggtgctggcg ttatagctgg atactggaag tggacatcag tgcgtttttc gaccatgtga
     1081 gacatgatct ggtgctcaaa gctctggaac accacgggat gcctaaatgg gtcatcctgt
     1141 actgtcgacg ctggatggaa gcgccaatgc agagttgtga aaatggagaa ttaataaccc
     1201 gaacacgagg cactccgcag ggcggggtca ttagtccgct acttgctaac ttgttccttc
     1261 actatgcatt tgatttgtgg atggaaagag aatatcgggg ggtaccgttt gagaggtacg
     1321 ctgacgatat tgtagtacat tgttcacgaa tgagtgatgc aacaaggcta aagaacaggt
     1381 tatcggagcg cttctcggaa gtcgggctgg tcctgaatgc agggaaaacg aatatcgcct
     1441 acattgacac gtttaaaagg agaaacgtcg caacgagttt cacctttctc ggatatgact
     1501 tcaaagtgcg tacgctgaag aatttcaaag gcgaacggta ccgaaaatgc atgccaggtg
     1561 cgtcaaatgc agcaatgcgc aaaataacag aaacaatcaa gaagtggcgt atacatcgct
     1621 caacagctga gagtttgctg gattttgcga ggcgctacaa tgcgatagtg agaggctgga
     1681 tcgagtacta cggaaagttc tggtccagaa atttcaacta tcgactgtgg agtgcaatgc
     1741 agtcacgtct gctcaagtgg atgcagtcta aatacagact ttcgaaccgg aaggctcagc
     1801 gaaagctgac gctggtaagg aaggagtatc cgaagctatt tgttcactgg tatttactac
     1861 gtgcatcaaa tgagtgatca agagccgtat gacgcgagag tgtcacgtac ggttccgtga
     1921 gcagccgggg agtgaaattc tctccggctg actcgactcg cgggttccga cagcgatgac
     1981 gaacatgcgg cggtgttgta ctcgctgatc ggcacatgcc gtctgaacaa tgtggaacca
     2041 gaaaaatggc tgcgttacgt cattgagcat atccaggact ggccggcaaa ccgggtacgt
     2101 gatctgttgc cctggaaagt tgatctgagc tctcagtaaa tatcaatacg gttctgacga
     2161 gccgcttact tttggcgtaa ttctgctgcg cctttactga acacaatgcc gtattcagca
     2221 agaagtccac gaatgtgatt tatcagttgt gtcctttcca ttgaaaccaa ccgacgcata
     2281 ttcaaaaaag tctggacgtc ctgttgctcc agagttttta ttggtacggg tcgtatagtt
     2341 ccatgttggc aggcctcagc tattgcctgt gcgtcgttat aatcattttt ctggcaccgc
     2401 tgataagctt taacatgttg tgccggaagc aatattacat catggtcgag tttcctgatt
     2461 tcccgggccc agtaatgaga tgccccgcca gcctccatcg caacagtcat tttaggctgt
     2521 tgcaccagcc agttaagcag ttgattgcga gaaagtttgg tctttcctgc gggtttgcca
     2581 tgagcattga tactgaaaag ataaaagttc gttttagcca gatcaatgcc ggtgattttc
     2641 tgttcagaca tggtatcccg cttacttttg tggttaaagt ttatccatca tggcgcaatt
     2701 gaaggctgcc gtgaaagagg gggaagacta tctcatcggt tactgttaaa cggctaagag
     2761 aaactatctt tttcaagcaa agaagatacg gtgaaacagg cgatttcacc gtgaaatact
     2821 gttcaaaaaa aacgctaata aaagcaaaat ttcttttaaa acagccaatt aaaaactggt
     2881 gagttgctgg cgaaaaactt gaattttcac agcgaattca gattatcttt tagtacaaga
     2941 gcaatcatac gatctaactt atcctgaact tccaacggta atctgccgaa ttcatatgag
     3001 aacgtacgtc cctttactcg ttttcgagca aatttatctt tactatcaaa tttccataac
     3061 aaagttacaa ccgctttatc ctttactcct gaatcaagca atgaagacat ttccttggtt
     3121 ataagccgca taatactgtt tttaacctcg tcttcagaaa gattgtcatc agcagtaata
     3181 agctcaatac tcggcgagat attcttcaca acttcatcaa tagatatatc acccagcctc
     3241 agtcgttcac ttaattcagc cagttgcctg taatcggcgt atgtcagctc agactgaaca
     3301 ggaaaaagag acacaaaatc tttaggtacg gacgctgctt ggagggcacg agtcacctta
     3361 gctgccgaca atccctctct ttccgcaatt tgcttctggt tcataccggc ctctttgaga
     3421 cgaactaaac gcaacccaat ttcacgaata ttatgttcaa gtgacgtctg caaatctttc
     3481 gccagatgct gagcttcaga aacagtaagc tcatcatccg ttacaagtac acgtaatcca
     3541 accttgcata gcaaagctgc tgcccgacga cgggaaccat ccaaaatctc aataagatcg
     3601 ccagtccgaa tcccaataca tggatagaat tgttgtaaac gtatagttct ggtgatgtct
     3661 tttagtgagt ccggcgtcag ggctgtctgt tcgcggccat tcacctcttc aaccacaaaa
     3721 gtttgagtgt ccactttgtc atgatcaacc ttgatctcag taaattttgc ctttcggcca
     3781 gtattgaggg taaatacatg aactggagcc gatatttctt cagtattatt caggatattt
     3841 gtattcagcg tacggccaat agtcggtcgg tgctttctgt tttccatcag tagttggccc
     3901 ttataaattc aatacgatca aaaactgctt tagcgaaatc ttctgctgcc atacgtgcat
     3961 ttttcaaagc ttcaccactg ccaacataag taacaggatt cgcagatatc acggtatcaa
     4021 acgactcgcc gcttctctca aaaccatcaa gacgtggcat tgaaacatca agcatgtcac
     4081 caccgaaaat ctcttttgtc aggctatggc agtatttatg atctgactta ttcgccagtt
     4141 tcgacatgaa gccaatattt gcctgcaaac ggcatgaaca tccatcctgt tcaataatct
     4201 gtacaagttc aggtaaacga gccaaatatt taagcgtaga atggaaatca acttgtgctg
     4261 gcggcaccgg tgtaaacatg atatctgcgg cagcaatggc atttttcaaa aatgcgtcca
     4321 gatgcgggcc tgtatcaata aggatgaagt cgaaatcatg cttcagctta tcaataatat
     4381 tctcacgcaa tatcgcgtgt ttgttttgtc ctaatagatg ctcctcgcac agagtatccc
     4441 agtttgatgc aataaaggcg tcgtcaatag aagccggcat aacataaacg ccagggataa
     4501 ctgacggaac gataaaatct tccagtaatt cctcacgact cacgttctgc aacatagcct
     4561 gcggtgcggt tgtatcaacc agccccaccg catgcaagta gttcagaaac atagtggctg
     4621 atgactgggg atctaaatca aggagcagaa tacgtaaatc ttctgacagc aggtgaggat
     4681 gagcacgcag ggcgtgagct acggaaacac tactcacggt tttagacaca ccgcctttta
     4741 aactaccgat gaatatggaa tatgcctcac tataacgatc gcgatattta ggaatgccac
     4801 gatgtgcata gatatcgata atgttctgga tggttaatgc atattttgtc gcggttcctg
     4861 ctgggcgttt ttcaaactga tagccctgag cctccatctc accgactgca ccatccacgc
     4921 ttgcgcgcgt taattttgga agcttagcta cagcagactt tgaatagact tgataaaaaa
     4981 catcagcctg aaactcctgc ttttgctcct gaatctgttt tgttagggcg agcaacattt
     5041 tatctgcacg ctgcgcaacc ttacttaact gctcaaaact agtcattccc cctccgtgct
     5101 atgtaggatt ttaacttatt acaaaaaaaa ctgcattagc agggtaatgc atgcaagtac
     5161 atattgtaca tgcttatatt aaaaatgcgc gcattgctga tttattaatc atcggaatga
     5221 atattactgt aagatagtat gcctttcggg gagaaagacg ctgatttccc ccgaataatc
     5281 cagttcacca ccacactccg gacaacagct ttcttcaggc tccagatggt gtatttcgcg
     5341 ggaaacatgt tccggtaacg gacggcgatg acgtgactgt cgcaacaggc gggggtagtg
     5401 cttccaatca gtagaacatg tgcttttcaa tgaaggagcc gatgactttt tcgtggattt
     5461 ccacggaacg cgagaagcag attgttttac gtgccagccg cttgatgcgg gtgcggaggg
     5521 tccggttgtt acgttcaata cgttgcgtga agattttgcc ggtcaggtgt ttctccttcg
     5581 gcacttctct ggcgtagctg ccccagtcat cgctggtcac catgccgata cagaacggag
     5641 tcaacagggc taacagttcc cgacaggtct catcattccg tggcccgaaa gtgtaagcca
     5701 acacaccgcc agttttagtg ttgtacgcat accagagcca gtgttgtgtc aacgacggat
     5761 gaaaagtgat ccacttatat ctccaccaac ggcccaatat tgatccaccg ttttactcag
     5821 gattagcttc tgctataacc ccggcctttc gtttctgtct gagtcgatag ctttctcctt
     5881 tgatttgaac gacatgtgag tggtgtaaga tacggcccag catcgctgag gtcagtgctg
     5941 catcaccggc gaacgtttga tcccactgcc cgaacggtag attggatgtc aggatcattg
     6001 cgctcttttc gtaacgttta gcgatgacct ggaagaacag ttttgcttct ttctgactga
     6061 acggcagata gcctatttca tcaatgatga gcaggcgggg ggccattact ccacgctgaa
     6121 gcg
//
LOCUS       DBKEKI010000200         6094 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.202, whole genome shotgun sequence.
ACCESSION   DBKEKI010000200 DBKEKI010000000
VERSION     DBKEKI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.202"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..225)
                     /locus_tag="ACUQPX_003995"
     CDS             complement(<1..225)
                     /locus_tag="ACUQPX_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HHV9058482.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIA"
     gene            412..1617
                     /gene="yedE"
                     /locus_tag="ACUQPX_003996"
     CDS             412..1617
                     /gene="yedE"
                     /locus_tag="ACUQPX_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HHV9058483.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            1614..1847
                     /gene="yedF"
                     /locus_tag="ACUQPX_003997"
     CDS             1614..1847
                     /gene="yedF"
                     /locus_tag="ACUQPX_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HHV9058484.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            1954..2624
                     /locus_tag="ACUQPX_003998"
                     /pseudo
     CDS             1954..2624
                     /locus_tag="ACUQPX_003998"
                     /inference="COORDINATES: protein motif:HMM:NF014626.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(2664..3872)
                     /locus_tag="ACUQPX_003999"
                     /pseudo
     CDS             complement(2664..3872)
                     /locus_tag="ACUQPX_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            3880..4309
                     /gene="tnpA"
                     /locus_tag="ACUQPX_004000"
                     /pseudo
     CDS             3880..4309
                     /gene="tnpA"
                     /locus_tag="ACUQPX_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            4883..5965
                     /gene="ompC"
                     /locus_tag="ACUQPX_004001"
     CDS             4883..5965
                     /gene="ompC"
                     /locus_tag="ACUQPX_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HHV9058485.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
BASE COUNT         1492 a         1484 c         1463 g         1655 t
ORIGIN      
        1 ggcaatcacc cgctgccact gacggcaatc cagggtatcg ccctctttag tgacaatcag
       61 gctgcctatc gcttccggac tgaccagcgc acgttgcggc cctttggtct gccagtagcc
      121 cgccagccag tccggcgcgg gggttttcac gacattgtta tagttttcga cttcggcaca
      181 gcccgccagc aacatgagcg cgcctgcaat tgcgagtttt ttcatcattc gtcctgcatg
      241 agaagaaaag aggattgtgg cattaaagct ctgatgtcgc cagcctttcc aggttcagag
      301 agaaatttga tctaactatt atttttgtga attattaccc cgaaaataaa tttgcatggt
      361 gtatgattcg cggctcttgt ttcaaacctt ctgagttcag aggctacatt catgtcatgg
      421 cagcaattca aacacgcctg gttgattaaa ttctgggcac ccatccctgc ggtcatcgcg
      481 gcgggtattc tctccactta ctattttggc attactggca ccttttgggc tgtcacgggt
      541 gaatttaccc gttggggcgg tcagctcctg cagctgtttg gcgtccatgc tgaagagtgg
      601 ggttatttta aaattatcca tctggaagga tcgccattaa cccgcatcga cggcatgatg
      661 atcctcggta tgtttggcgg ctgctttgct gctgcgctgt gggccaacaa tgtcaaacta
      721 cgcatgccac gcagccgtat ccgcattatg caggccatca ttggcggcat tatcgccggt
      781 tttggcgcgc gtctggcaat gggctgtaac ctggcggcgt tctttaccgg cattcctcag
      841 ttctcgctgc atgcctggtt ctttgccatc gccactgcca ttggttcatg gtttggcgcg
      901 cgctttaccc tgctgcccat cttccgtatt cccgtgaaaa tgcagaaagt ttctgccgcc
      961 tcaccgctga cgcaaaaacc ggatcaggcg cggcgtcgtt ttcgtctcgg gatgctggtc
     1021 tttttcggca tgctgggctg ggcgctgctc acggcgatga accaaccaaa actggggctg
     1081 gcaatgctgt ttggcgtcgg ctttggttta ctgattgaac gtgcgcaaat ctgctttact
     1141 tcggcgttcc gcgatatgtg gatcaccgga cgtacccata tggcgaaagc aatcattatc
     1201 ggtatggcgg tgagtgccat cgggatcttc agttacgtac agttaggcgt tgaacctaaa
     1261 atcatgtggg cgggaccaaa tgcggtaatt ggtggtttac tttttggttt tggcatcgtg
     1321 ctggcaggcg gctgcgaaac cggctggatg taccgcgcgg tagaaggcca ggtgcactac
     1381 tggtgggtcg gtctgggcaa cgtgatcggc tcaacgattc tggcgtatta ctgggatgat
     1441 ttcgctccgg cgctggccac cgactgggac aaaatcaacc tgctgaaaac ttttggtccg
     1501 atgggaggcc tgctggtgac atatttgctg ctgtttgctg cgctgatgtt gattatcggc
     1561 tgggaaaaac gcttcttccg ccgtgcggca ccgcagactg ctaaggagat cgcatgaaaa
     1621 atatcgttcc tgattaccgc cttgatatgg tgggtgaacc ctgcccttat ccggcagtcg
     1681 caacccttga ggcgatgccg caattaaaaa aaggtgagat cctggaagtg gtgagcgact
     1741 gtccgcagtc gatcaataat attccactgg atgcgcgtaa tcacggctac acggtgctgg
     1801 atattcagca agacgggccg accattcgtt atttaattca gaagtaatcc ctcattcccg
     1861 ctgtggccca cagcgggaac atcgttacct ctgacacata gattttaccc gccccctctc
     1921 ctattctttt cagtatgatc tcaggaggtt gctatgtgtg gacgctttgc ccaatcccaa
     1981 acgcgtgagg attaccttgc gcttctcgcg gaagatattg aacgcgatat tccctacgat
     2041 cccgaaccca ttggcagata caatgtcgcg ccgggaacca aagttcttct gctcagtgaa
     2101 cgtgatgaac accttcatct ggatccggtt ttctggagat atgctcccgg atggtgggat
     2161 aaaccgccgc tgattaacgc ccgcgtagaa actgcctcaa ccagtcgcat gtttaaaccg
     2221 ctctggcaac atggtcgggc aatctgtttt gccgatggct ggtttgagtg gaaaaaagaa
     2281 ggcgacaaaa aaagccttat tttatctatc gcgctgacgg acaacctgtt tttatggccg
     2341 cgataggtag cataccgttt gagcgtggtg acgaagccga aggatttttg atcgtcactg
     2401 cggcggcaga tcaaggtctg gtagatattc atgaccgccg cccgctggtt ctgtcgccag
     2461 aagctgcacg ggaatggatg cggcaagaca ttggtggtaa agaagcctca gaaatcgcta
     2521 ccagaagctg tgtgccagca aaccagttca cctggcaccc tgtgtcgcgc gcggtgggta
     2581 atgtcaaaaa ccagggcgcg gagttaattc aacctgtttg ttgacatcct ccacgccctg
     2641 aatgacgagg acccctgcta cgttcaggct gtcgcctgaa tcatttcggt gggttcctgc
     2701 ttcaacgagc ggcctgactg caccatctct ccacaggcaa gaacggcgtg ccccgccgct
     2761 aaaatgttac gagcgccatt tacatcggcg ttcgctgtat atccacatac ctggcatctg
     2821 aatttacttt gtgacaggcg attttctttc gctgtatgac cacagcacgc gcaacgctgg
     2881 cttgtgtacg ctggcggaac agcaagcacc tgaccgccac gccagagctg cttgtactca
     2941 agctggcggc gcatttcata ccagccctga tccagtatcg aacggtttaa acctgatttt
     3001 gcccggacat tgcgccccgg ctggctgacc gtacccgctg ctgactttga catgttgctg
     3061 accttcaaat cctcaatgac tatcattacg tggtttttgc tgacggtcgt tgtgactttg
     3121 tgaaggtagt ccctgcggat atttgcgata caggaatgca gtcgctgtat tttgcgtttc
     3181 tgcttctgcc agttgttgct gaatttgacc ttgcggctta actgtcgctg aagtctcgcc
     3241 agcttcttct ggtttttctg aaaactgttt acaggctcaa agactgtgcc atctgacagc
     3301 gaggcaagtt tagccacgcc agcatccagc ccgaccattg atgctgaagg gtgaaccgga
     3361 gttgatactt cactttctgt ctgaatactg atgtaccact taccgcagga ctggctgaca
     3421 gtgacatttt tcacaacacc cgtgacctga cggctgttcc ggtagcgcat ccagccaagt
     3481 tttggcagaa aaatacggct gttttcctga gcgagcttaa cgccttgcgg atagcggaat
     3541 gcatcattct gtcaccgctt tttgaatcag gggaacgccg accgcttctg gaaaaagttt
     3601 ttgcaggcct gctcagggtc tttcagtgac tgttgcaatg gctgtgacgg agcatcttta
     3661 atccattgcg tttcagtggc atttttccac tcaaccaatc aggaagccat tttcgcgtaa
     3721 gggatgtctt tgttaccggc cttatgattc tcattctgac gtgccagagt acgactgaaa
     3781 acgaaacgac atgcgcctgc gaagcgcctc atttcacgcg cctgttgacc accgggtctt
     3841 aaccggaatt tgaatacctg tagtcgtttc acagggcaaa ttatactttg gtctgtgaaa
     3901 aaaactgata tccgacgcgg aaggcactgt gttttcctga tgcatgctca ccgggtcttt
     3961 gttaccaagt accgacgcca gatttttgac catgacgcga cagaaaaact acgtacttac
     4021 ttttcaaatg tatgtgctga ttttgaagct gaactggttg aaatggatgg cgaaccagag
     4081 cacgtccatc tgttaattaa ctatcccccc aaactggcga tatccagtct ggtaaacaaa
     4141 cagcctcaaa ggcgtatcgg gtaggttact gcgacgggat cgaccagata ttgctgccag
     4201 gtattaccgc aaagatgttt tgtggcgtcc cggctatttt gccagtagct gcggaggtgc
     4261 gccaatatcc gtcatccgcc aatacattga acaacagcaa acacctggtc aggtgaaaaa
     4321 acgagcctta tatccccgcc ctgaaggacg ggcttttacg gtgcactgga taagcctgga
     4381 gattatctac acctgcatgg acatcattgt actatgcccc gtacatgccc catcacatcc
     4441 tcaacatcaa agtattagag ccttgcaaac cattatgcgt ctcagtttgt ctcatctatc
     4501 ttacgcaaaa aaaatagaac taatatcaag cggcgctata ttgcgtatta aataaaacca
     4561 cctcaattac attaataaat ctaaataaat atcatgcaaa taatttccat ggttattttt
     4621 aggctattta aattaaaaaa ataaattatg agtcaatcat aaataacaac ctttaatata
     4681 atttgactac caaatttata ttcgttcaca aaatagtcat gcattgtgtg caatagaaac
     4741 agttcagata aaaataggga tctacttcac aatttaaatg agaaactaaa acttacatct
     4801 tgaaataatc tcattgattg gatgaatatt tgtagtgcag tgacatcatt tttaaataat
     4861 agttcaaata aaagggctca cgatgaaaaa attaacagtg gcaatttctg ctgtagctgc
     4921 atcagtactg atggcgatgt ctgctcaggc agcagagatt tacaataaag acagtaacaa
     4981 gctggatctg tacggaaaag ttaatgctaa gcactacttc tcttctaacg atgcagatga
     5041 tggcgacaca acttatgttc gtctgggctt caaaggcgaa actcaaatca acgatcaact
     5101 gactggtttc ggtcagtggg aatatgaatt caaaggcaac cgtgctgaat ctcaaggttc
     5161 ttccaaagat aaaacccgtc ttgccttcgc tggcctgaaa ttcggtgact acggctccat
     5221 cgattatggc cgtaactacg gtgtagcata cgatatcggt gcatggaccg acgttctgcc
     5281 agaattcggt ggcgatacct ggacccaaac agatgtgttc atgactggtc gcaccacagg
     5341 tgttgcaacc tatcgtaaca acgacttctt tggtctggtt gatggtctga actttgctgc
     5401 tcagtatcag ggtaaaaatg accgcactga cgtaactgaa gctaacggtg atggtttcgg
     5461 tttctccact acttatgagt atgaaggatt cggtgtaggt gcaacctatg ctaaatctga
     5521 ccgcactaat gatcaggtta tctacggtaa caacagcctg aatgcatctg gtcaaaatgc
     5581 tgaagtatgg gcagctggtc tgaaatatga tgcgaacaac atctatctgg ctaccaccta
     5641 ttctgaaacc cagaacatga ctgtttttgg taataaccat attgccaaca aagcacaaaa
     5701 cttcgaagta gttgcacaat atcagttcga cttcggtctg cgtccgtccg ttgcttacct
     5761 gcaatctaaa ggaaaagact tgggtgcgtg gggtgatcag gacctgattg aatatattga
     5821 tgtaggtgca acctattact tcaacaaaaa tatgtccact tttgttgatt acaaaatcaa
     5881 cctgattgat aagagcgatt tcacgaaagc atctggcgtt gctaccgatg atatcgttgc
     5941 tgtaggattg gtttaccagt tctaatctga ttactcaaag atatgttgtg ggaggctttg
     6001 cctccccaac atataagtgg cgccctcaag ccactttcct tagaagcact atcttgcttc
     6061 tgactatata aaccttctgt tatatattac cctt
//
LOCUS       DBKEKI010000201         6072 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.203, whole genome shotgun sequence.
ACCESSION   DBKEKI010000201 DBKEKI010000000
VERSION     DBKEKI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.203"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..569
                     /gene="yneK"
                     /locus_tag="ACUQPX_004002"
     CDS             <1..569
                     /gene="yneK"
                     /locus_tag="ACUQPX_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HHV9058486.1"
                     /translation="HHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKD
                     PNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPKVCICFLSLLAEQESPHFQDLFL
                     FFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHE
                     DLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            719..1909
                     /gene="ydeA"
                     /locus_tag="ACUQPX_004003"
     CDS             719..1909
                     /gene="ydeA"
                     /locus_tag="ACUQPX_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HHV9058487.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(1934..2599)
                     /gene="marC"
                     /locus_tag="ACUQPX_004004"
     CDS             complement(1934..2599)
                     /gene="marC"
                     /locus_tag="ACUQPX_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HHV9058488.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2811..3245
                     /gene="marR"
                     /locus_tag="ACUQPX_004005"
     CDS             2811..3245
                     /gene="marR"
                     /locus_tag="ACUQPX_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HHV9058489.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3265..3648
                     /gene="marA"
                     /locus_tag="ACUQPX_004006"
     CDS             3265..3648
                     /gene="marA"
                     /locus_tag="ACUQPX_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HHV9058490.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3680..3898
                     /gene="marB"
                     /locus_tag="ACUQPX_004007"
     CDS             3680..3898
                     /gene="marB"
                     /locus_tag="ACUQPX_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HHV9058491.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(3929..4756)
                     /locus_tag="ACUQPX_004008"
     CDS             complement(3929..4756)
                     /locus_tag="ACUQPX_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HHV9058492.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATLVPVVGLASAALLLDERLTGLQFLGAVLIMTGLYINVFGLRWRKAVKVGS"
     gene            4951..>6072
                     /gene="ydeE"
                     /locus_tag="ACUQPX_004009"
     CDS             4951..>6072
                     /gene="ydeE"
                     /locus_tag="ACUQPX_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HHV9058493.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNNLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVII"
BASE COUNT         1546 a         1529 c         1394 g         1603 t
ORIGIN      
        1 atcaccatac cgctgcagat tacttacgcc agtttgattt gcttgtcctg aattttatct
       61 ctcgcggtaa ttttgtcata ctcccccgat tatggaataa ctctgaggtt cacagatggt
      121 tcgtcaataa agatcctaac ttgatcaccg ccattctcga cataacggac agcgaattaa
      181 aagaggattt attgcaaagc ctgatggatt cattaggttc taacaaacat gtacaaccca
      241 aagtctgcat ctgcttctta tcccttttag cagaacaaga atctcctcat tttcaggact
      301 tgtttttatt tttcgccaat atgttattgc actatcacca atttatgaat cccaatgaaa
      361 gtgatctgaa tgacgtgttg atgccagcat cattaagtga tgataaaatt atcaaacata
      421 tggcgcgcag gaccctcaaa ctgtttgtaa aaaatgaaac gccgccaaaa gttactcacg
      481 aagacctggt gaaaaacagg cctcgctccc ctgtcagacc acctataccc gcaaccgcca
      541 aaacgccaga cctccctgaa cgtcattaaa ccgtgatgtt accgactctc tgacgcggaa
      601 cagaatcagc gtcagagaaa cggaaaacgc gatccagatc acaaatgcat tgtattcaca
      661 tcattaaccg ttttaagatc atttcatcac tttttcgcaa ctcacccgat aatctgttat
      721 gacaacaaac actgtttccc gcaaagtggc gtggctacgg gtcgttacgc tggcagtcgc
      781 cgccttcatc ttcaacacca ccgaatttgt ccctgttggc ctgctctctg acattgcgca
      841 aagttttcac atgcaaaccg ctcaggtcgg catcatgttg accatttacg catgggtagt
      901 agcgctaatg tcattgcctt ttatgttaat gaccagtcag gttgaacggc gcaaattact
      961 gatctgcctg tttgtggtgt ttattgccag ccacgtactg tcgtttttat cgtggagctt
     1021 taccgttctg gtgatcagtc gcattggtgt ggcttttgca catgcgattt tctggtcgat
     1081 tacggcgtct ctggcgatcc gtatggctcc ggccgggaag cgggcacagg ccttgagttt
     1141 aattgccacc ggtacagcac tggcgatggt cttaggttta ccgctcgggc gcattgtggg
     1201 gcagtatttc ggctggcgaa tgaccttctt cgcgattggt attggggcgc ttgttaccct
     1261 tttgtgcctg attaagttac ttcccttact gcctagtgag cattccggtt cattgaaaag
     1321 cctcccgcta ttgttccgcc gcccggcatt gatgagcatt tatttgttaa ctgtggtggt
     1381 tgtcaccgcc cattacacgg catacagcta tatcgagcct tttgtgcaaa acattgcggg
     1441 attcagcgcc aactttgcca cggcattact gttattactc ggtggtgcgg gcattattgg
     1501 cagcgtgatt ttcggtaaac tgggtaatca atatgcgtct gcgttggtaa gtacggcgat
     1561 tgcgctgttg ctggtgtgtc tggcactgct gctacctgcg gcgaacagtg aaatacacct
     1621 cggggtgctg agtattttct gggggatcgc gatgatgatc atcgggcttg gtatgcaggt
     1681 taaagtgctg gcgctggcac cagatgctac cgacgtcgcg atggcgctat tctccggcat
     1741 atttaatatt ggaatcgggg cgggtgcgtt ggtaggtaat caggtgagtt tgcactggtc
     1801 aatgtcgatg attggttatg tgggcgcggt gcctgctttt gccgcgttaa tatggtcaat
     1861 cattatattt cgccgctggc cagtgacact cgaagaacag acgcaatagt tgaaaggccc
     1921 attcgggcct tttttaatgg tacgttttaa tgatttccaa gatgccgtta ataataaact
     1981 gcacacccat acataccagc aggaacccca tcaggcggga gatcgcttca atgcctccct
     2041 tgcccaccag ccgcataatt gcgccggagc tgcgtaggct tccccacaaa ataaccgcca
     2101 ccaggaaaaa gatcagcggc ggcgcaacca tcagtaccca atcagcgaag gttgaactct
     2161 gacgcactgt ggacgccgag ctaataatca tcgctatggt tcccggaccg gcggtacttg
     2221 gcattgccag tggtacaaag gcaatattgg cactgggttc atcttccagc tcttccgact
     2281 tgcttttcgc ctccggtgaa tcaatcgctt tctgttgcgg aaaaagcatc cgaaaaccga
     2341 taaacgcgac gattaagccg cctgcaattc gcagaccggg aatcgaaatg ccaaatgtat
     2401 ccatcaccag ttgcccagcg taatacgcca ccatcatgat ggcaaatacg tacaccgagg
     2461 ccatcaacga ctgacgatta cgttcagcac tgttcatgtt gcctgccagg ccaagaaata
     2521 gcgcgacagt tgttaatggg ttagctaacg gcagcaacac caccagcccc aggccaattg
     2581 ctttaaacaa atctaacatt ggtggttgtt atcctgtgta tctgggttat cagcgaaaag
     2641 tataaggggt aaacaaggat aaagtgtcac tctttagcta accttgcatc gcattgacca
     2701 aaacttgaac cgatttagca aaacgtggca tcggtcaatt cattcatttg acttatactt
     2761 gcctgggcaa tattatcccc tgcaactaat tacttgccag ggcaactaat gtgaaaagta
     2821 ccagcgatct gttcaatgaa attattccat tgggtcgctt aatccatatg gttaatcaga
     2881 agaaagatcg tctgcttaac gagtatctgt ctccgctgga tattaccgcg gcacagttta
     2941 aggtgctctg ctctatccgc tgcgcggcgt gtattactcc ggttgaactg aaaaaagtgt
     3001 tgtcggtcga cctgggagca ctgacccgta tgctggatcg cctggtctgt aaaggctggg
     3061 ttgaaaggtt gccgaacccg aatgataagc gcggcgtact ggtaaaactt accaccagcg
     3121 gcgcggcaat atgcgaacaa tgccatcaat tagttggcca ggacctgcat caagaattaa
     3181 caaaaaacct gacggcggac gaagttgcaa cacttgagca tttgcttaag aaagtcctgc
     3241 cgtaaacaaa aaagaggtat gacgatgtcc agacgcaata ctgacgctat taccattcat
     3301 agcattttgg actggatcga ggacaacctg gaatcgccac tgtcactgga gaaagtgtca
     3361 gagcgttcgg gttactccaa atggcacctg caacggatgt ttaaaaaaga aaccggtcat
     3421 tcattaggcc aatacatccg cagccgtaag atgacggaaa tcgcgcaaaa gctgaaggaa
     3481 agtaacgagc cgatactcta tctggcagaa cgatatggct tcgagtcgca acaaactctg
     3541 acccgaacct tcaaaaatta ctttgatgtt ccgccgcata aataccggat gaccaatatg
     3601 cagggcgaat cgcgcttttt acatccatta aatcattaca acagctagtt gataacgtga
     3661 caacgtcact gaggcaatca tgaaaccact ttcatccgca atagcagctg cgcttattct
     3721 cttttccgcg cagggcgttg cggaacaaac cacgcagcca gttgttactt cttgtgccaa
     3781 tgtcgtggtt gttcccccat cgcaggaaca accaccgttt gatttaaatc acatgggtac
     3841 tggcagtgat aagtcggatg cgctcggcgt gccctattat aatcaacacg ctatgtagtt
     3901 tgttctggcc ccgacatctc ggggcttatt aacttcccac ctttaccgct ttacgccacc
     3961 gcaagccaaa tacattgata tacagcccgg tcataatgag caccgcacct aaaaattgca
     4021 gacccgttaa gcgttcatcc aacaatagtg ccgcacttgc cagtccgact acgggcacca
     4081 gtgtcgccac aaacgccaga tacatcagcg acaagatggt ggtcatatca atagtaacca
     4141 gactgtgaat catggatgcg gaaccatcga gaatcagcga ggcaacaaag aagggaatga
     4201 ttgggattaa agcgctccag attaccagcg acatcaccgc cggacgcgtt gagtgcgaca
     4261 tgatcttttt attgaagatg ttgccacacg cccaactaaa tgctgcagcc agggtcaaca
     4321 taaagccgag catcgccaca tgctgaccgt tcagactatc ttcgattaac accagtacgc
     4381 caaaaatcgc taaggcgatc cccgccaatt gtttgccatg cagtcgctcc ccgaaagtaa
     4441 acgcgccaag cacgatagta aaaaacgcct gtgcctgtaa caccagcgaa gccagtccag
     4501 caggcatacc aaagttaatg gcgcagaaca gaaaggcaaa ctgcgcaaaa ctgatggtta
     4561 atccataccc caagagcaaa ttcagtggta ctttcggtcg tgcaacaaaa aagatggccg
     4621 gaaaagcgac cagcataaag cgcaaaccgg ccagcatcag cggtggcatg ttatgaagcc
     4681 ccactttgat gaccacaaaa tttagccccc atacgaccac taccagtagc gccaacaccc
     4741 catcttttcg cgacattcta ccgcctctga atttcatctt ttgtaagcaa tcaacttagc
     4801 tgaatttact tttctttaac agttgattcg ttagtcgccg gttacgacgg cattaatgcg
     4861 caaataagtc gctatacttc ggatttttgc catgctattt ctttacatct ctaaaataaa
     4921 acataacgaa acgcactgcc ggacagacaa atgaacttat ccctacgacg ctctaccagc
     4981 gcccttcttg cctcgtcgtt gttattaacc atcggacgcg gcgctacgct gccatttatg
     5041 accatttact tgagtcgcca gtacagcctg agtgtcgatc taatcggtta tgcgatgaca
     5101 attgcgctca ctattggcgt cgtttttagc ctcggttttg gtatcctggc ggataagttc
     5161 gacaagaaac gctatatgtt actggcaatt accgccttcg ccagcggttt tattgccatt
     5221 cctttagtga ataacgtgac gctggttgtg ctcttttttg ccctcattaa ctgcgcctat
     5281 tctgtttttg ctaccgtgct gaaagcctgg tttgccgaca atctttcgtc caccagcaaa
     5341 acgaaaatct tctcaatcaa ctacaccatg ctaaacattg gctggaccat cggtccgccg
     5401 ctcggcacgc tgttggtgat gcagagcatc aatctgccct tttggctggc agctatctgt
     5461 tccgcgtttc ccatgctttt cattcaaata tgggtaaagc gcagcgagaa aatcatcgcc
     5521 acggaaacag gcagtgtctg gtcgccgaaa gttttattac aagataaagc actgttgtgg
     5581 tttacctgct ctggttttct ggcttctttt gtaagcggcg catttgcttc atgcatttca
     5641 caatatgtga tggtgattgc tgatggggat tttgccgaaa aggtggtcgc ggttgttctt
     5701 ccggtgaatg ctgccatggt ggttacgttg caatattccg tgggccgccg acttaaccca
     5761 gctaacatcc gcgcgctgat gacagcaggc accctctgtt tcgtcatcgg actggtcggt
     5821 tttatttttt ccggcaacaa cctgctattg tggggtatgt cagctgcggt atttactgtc
     5881 ggtgaaatca tttatgcgcc gggcgagtat atgttgattg accatattgc gccgccagga
     5941 atgaaagcca gctatttttc cgcccagtct ttaggctggc ttggtgccgc gattaaccca
     6001 ttagtgagtg gcgtagtgct aaccagcctg ccgccttcct cgctgtttgt catcttagcg
     6061 ttggtgatca tt
//
LOCUS       DBKEKI010000202         5972 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.204, whole genome shotgun sequence.
ACCESSION   DBKEKI010000202 DBKEKI010000000
VERSION     DBKEKI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.204"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..145)
                     /locus_tag="ACUQPX_004010"
     CDS             complement(<1..145)
                     /locus_tag="ACUQPX_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HHV9058494.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFA"
     gene            complement(204..1553)
                     /gene="chbC"
                     /locus_tag="ACUQPX_004011"
                     /pseudo
     CDS             complement(204..1553)
                     /gene="chbC"
                     /locus_tag="ACUQPX_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(1638..1958)
                     /gene="chbB"
                     /locus_tag="ACUQPX_004012"
     CDS             complement(1638..1958)
                     /gene="chbB"
                     /locus_tag="ACUQPX_004012"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HHV9058495.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(2257..2595)
                     /gene="osmE"
                     /locus_tag="ACUQPX_004013"
     CDS             complement(2257..2595)
                     /gene="osmE"
                     /locus_tag="ACUQPX_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HHV9058496.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            2797..3624
                     /gene="nadE"
                     /locus_tag="ACUQPX_004014"
     CDS             2797..3624
                     /gene="nadE"
                     /locus_tag="ACUQPX_004014"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HHV9058497.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVAYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            3854..4741
                     /gene="cho"
                     /locus_tag="ACUQPX_004015"
     CDS             3854..4741
                     /gene="cho"
                     /locus_tag="ACUQPX_004015"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HHV9058498.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(4701..5276)
                     /gene="ves"
                     /locus_tag="ACUQPX_004016"
     CDS             complement(4701..5276)
                     /gene="ves"
                     /locus_tag="ACUQPX_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HHV9058499.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGELSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(5479..5964)
                     /gene="spy"
                     /locus_tag="ACUQPX_004017"
     CDS             complement(5479..5964)
                     /gene="spy"
                     /locus_tag="ACUQPX_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HHV9058500.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEECRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1536 a         1482 c         1424 g         1530 t
ORIGIN      
        1 cggcaaaatc gccctgcttc gcctgtttca atgcggcata cgccaggcta cgcgcttgtc
       61 cagagttgat gataagcccc atcaccactt cttccagctc ttcagcttct gtttgcgtat
      121 caggaatatt atcgagatcc atcatacatc gttcctctct cttcttaccg gcaagattac
      181 ccgtaccggc atcgattaaa atttcagggg ttagcgatat cttcttcgct ctcttcttta
      241 tcaatcgcat tttgtgcttt gttagccacc acaacaaagg gcagataaat taacgttgcg
      301 atgccaaggt tgaagagtgc gaccagcaat gcggcgacgc taccgttggt gttaaagaag
      361 gctcccagac cggttggcat ggtccacggt gcaatattgg tcaccggagg aatgatgccc
      421 atgtagtacg ctgcgagggt gattgccgcc agaatcggtt gtaccagtac aaacgggata
      481 aacatcaccg ggttcatgat aattggcaga ccaaacagaa tcggttcgtt aatctggaag
      541 atgccggacg gcagcgccag ttttgccacc tgacgataat cagcacggcg agaggcgata
      601 aagatagcca ggatcaggcc taaagtcgca ccactgcccc caaggaaaat aaaggagtcc
      661 agcatcggct tggctcagat atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa
      721 ccatattgct gataggtcgc gatattttcc agcgcccacg gcgtcataat gccgttgtcc
      781 agtgcggtca gcgccagcgc gccatgaata ccgaagaacc agagtaatgg aacaaagatc
      841 acataggccc agcccaccac gctacccaac gatgccagtg gggttgagat ggtatccata
      901 atgatctgat ggaagttggt gccccaggta ttcaacgccc aggcaataat ccccatcacg
      961 gaaagaataa taaagccggg aattaatgcc gagaaagaac gcgatactga agcaggtacg
     1021 ctgtcgggca gtttaatgac ccaattgcgg cggacaataa aggtaaacat ttctgccacc
     1081 accaggccaa taataatccc ggagatgata ttcgccccac ctaaccagtt tgcgccaacc
     1141 gcataggcct caccgacact atatggggtg acggtcataa atgctgcaac ggataacaac
     1201 ccagccgcca gcgcatcgac tttacgctct tctgccagcg ccatgccaat aaagaacggt
     1261 gccattaaag acattattcc taatgttccg ttatatacgt tgccgccaat acctttcaga
     1321 ccattaagtg tttcaatggt tgaggcgtcg aggcgaatac ctaaggaata aaaaaacgac
     1381 ccctccccaa agcttagaaa aacgttgtta attaatacaa acatggcccc cgcaagggct
     1441 aacggcatta agcgaataaa gccatttttg attgcattaa cgtgtggctg ctttcctatt
     1501 ttaactgcaa aagggaggag taccttttca agcgatgcaa taacattact catagaaaaa
     1561 tacccttaaa aaccgcaatt taaatattgc ggtattgatt tatgaaataa ctctttgacg
     1621 ggaaaattta aaataaatta atttgctgcg gcttttttaa tcgctgcaac cgcagcctta
     1681 agcacgccta aaccatcgac tttgccataa agcagcgagt caattacttc aaccggtttg
     1741 ttgggtaaca aacgctggat ttcgggcaac atataagcaa tctgcggccc taataacacg
     1801 acatcggcat tctgaccttt ttcaccagcc agtgtttccg gaaatgcttc aataatgacc
     1861 ggaacttcat atttttctgc ctgtgcgcgc atttttgata ccagtaaaga gatagacatg
     1921 cccgcagaac aaaacagata aatgtgtttc ttttccataa aactgccctc atcgacgatt
     1981 atctgtcagc cagacactcc gcaagcctta acctgcttcc atgctctggg taacttgcga
     2041 aaccaacatg actttttttg tggctgaaag gagtatacgg tatagacctg agcaatagta
     2101 tttctttgac cttataaatt gtttctcctt gacctgtggt tatgaccctc ttcacatttc
     2161 gggcaaataa ttcgcgaaga taattaagaa ctcaggccaa aaaaaaccgg cgcaatggcc
     2221 ggtttccgtt gttactcaag ctttcagccg aattgattac ttcgcagcct gtggatcagt
     2281 gtcgtattca gcacaggtct gataaccgga gttgatgaca tgtccggtat catctaacgc
     2341 gacaaagtag gtttctgctt taccatcacg ttgacccagg atgtaggtct ggcaagtacc
     2401 gcgagcatgg atcatgctca cttcagacga aggtttaccc gcaatttgtg caacctgcgc
     2461 ccggctcatg ccttttttga catctttcac cacaggctgt acaaactggt ctttggtacg
     2521 atcataagct gtacaacccg ccagcatggt taataccgcc gctgcactca gaattcctgc
     2581 catattcttg ttcatattcc gtcctcttgt ttatcagcgt gttagataag cctggaatac
     2641 attgggcgct ttttcaagcc cgtgaacgaa acggctccgc tttcagagga ttcctgtatg
     2701 acgttttaac caccattcag cccgctgtcg cttgtcgttt cagtagcaac gggttagctt
     2761 taaggaagtt ttgtcttttc tgtctggagg ggttcaatga cattgcaaca acaaataata
     2821 aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg aaattcgtcg tagtgtcgat
     2881 tttctgaaaa gctacctgca aacttatccg ttcattaaat cactggtgct cgggatcagc
     2941 ggcggtcagg actccacgct tgccggaaag ctgtgccaga tggcgattaa tgagctgcgc
     3001 caggaaactg gcaacgaatc actgcaattt attgccgtac gcctgcccta tggtgttcag
     3061 gctgacgaac aagattgcca ggatgccatt gcctttattc aaccggatcg cgtattaacc
     3121 gttaatatca agggcgcggt attggctagc gagcaggcat tgcgggaagc aggcattgaa
     3181 ctgagcgatt ttgtccgtgg caatgaaaaa gcgcgtgagc ggatgaaagc acaatatagc
     3241 attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc atgcagcaga agccattacc
     3301 ggattcttca ctaaatatgg tgacggcggt acggatatta atccgctgta tcgtctcaac
     3361 aaacgccagg gtaaacagtt actgacggca ttaggttgcc cggaacacct ttataagaaa
     3421 gccccaactg ccgatctgga agatgatcgc ccttctctgc cagatgaagt ggcactcggc
     3481 gtggcctatg acaatatcga cgactatctg gaagggaaaa acctacctga acaggtcgcc
     3541 agaacaatag agaactggta tctgaaaacc gaacataaac gccgtccgcc aattaacgtt
     3601 ttcgatgatt tctggaaaaa gtaataattt gcacctattg gattgtgcga aaaagagtaa
     3661 tttgttcacg ccggatgcgg cgtgaacgcc ttattcgacc tacaaaacta tgcaaattca
     3721 atacattgca ggagtcgaat aggcctgaca ggcgtagcac gtcagacggt gtaacctttg
     3781 tcatcaaccc gcctcttttt taatcgcctc ccgcctgtta cactggatag ataaccagca
     3841 ttcggagtca acagtggtac ggcgtttaac ttctccgcgg ctcgaatttg aagctgcggc
     3901 aatttatgaa tatcccgaac atttacattc attccttaat gacttaccca cccgaccagg
     3961 ggtgtatctg tttcatggcg aaagtgacac catgccgctc tatatcggca aaagcgttaa
     4021 tatccgcagt cgggtgcttt ctcatttacg caccccggat gaagccgcca tgctacggca
     4081 atcccggcga attagctgga tctgcaccgc aggcgaaatc ggcgctctgc tccttgaagc
     4141 gcgattaatc aaagaacaac agccgctgtt taataaacgg ttgcgccgca atcgccagct
     4201 ctgtgccctg caattaaatg aaaagcgcgt cgatgtggtg tatgccaaag aggtggattt
     4261 ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg cgcgcagctt tgcaagcatt
     4321 gcagagcatc gctgatgaac aaaaactttg ttatggcctg ttgggactgg aaccgttaag
     4381 tcgcggtcgt gcatgttttc gttcagcgct aaaacgttgc gccggagcat gctgcggtaa
     4441 agagagccat gaggaacatg cgctacgctt gcgccagtct ctggagcgtt tgcgggtggt
     4501 gtgttggcct tggcaaggtg cggtggcact gaaagaacag cacccggaaa tgactcaata
     4561 tcatattatt caaaactggc tgtggctggg ggcggttaat tcgctggaag aagcgacaac
     4621 gttaattcgg acacccgccg ggtttgatca cgacggttat aaaattcttt gtaagccgct
     4681 gctttccggt aactatgaaa ttactgaact tgatccggcg aatgaccagc gagccagttg
     4741 atttcactga acaacagttt accttgcggt tgtaaaagcc gcagggtgtg cctgccatcg
     4801 aaccagcacg cgccctggtc ggtagtgagc agtttgtcgc ccagttgcca cgccccgtta
     4861 atcacaaaca ccaccccgcc gcgtgagcca aaggtagtaa acgtacgttc ggcaattctg
     4921 actttcgcct tacagacatc caggcgcgtc ataatattga aatccatcga catctgccct
     4981 gccgtcagtt tcgcttttac cacctggtcc gccgtaaagg caaaaggctg taacggtttt
     5041 aaggtatggt taaagcgatc tgcgctttca aggagcatct cgccgccttc cagcaacgtc
     5101 actatccttt ccatgccggg aaacaaagaa agctccccat tcgccgcgat ggatgcaatg
     5161 ctggcacgcc agtaaaaatc acgttttgcc ggagggaaag tgcaaatttc gcgcgtttca
     5221 ccggcagcgt ttcgccacag attcaccgac attttacgca tatcaaagta ttccatactc
     5281 gctcccggcc cgttcttttc attgttatcg ttcaggtaac cgtggatggt tatcggcaac
     5341 tcgggtgatt tacgttagtg gtgatcaggc tttttttaca actgtttgag aagagaaaag
     5401 aaaaccgccg atcctgtcca ccgcattact gcaaggtagt ggacaagacc ggcggtctta
     5461 agttttttgg ctgaaagatt attcagcagt tgcaggcatt ttaccttttg ccgctggacg
     5521 ttctgtcaga cgcttctcaa aattagcatt aaattgcttt ttctgttccg gcgtcaggat
     5581 gttgtaaatt ttgttctggg tttccatgtg cgccagcatg ttagctttgc gctgttcttc
     5641 catttttgcg atctgcgctt cagcttttgc tttatcgaag gtatcgctgg caatgatgtc
     5701 atgcattgcg cggcattctt ccagcggcgg acgtttcatc tggtcacgct ggcctttcat
     5761 gatttcgtgg atctgctgtt tctgcgcgtc ggtcaggttc aggtctttga acatcatgtc
     5821 ctgatgcgga ccgaacttgc ctttgtggtg catcatcggc ttcgcgtcag ccggtgctgc
     5881 ggtagtggtg tctgcggcat gggccaggtt agccgcgcca agagccaggg tagaggcaac
     5941 aaacagtgca gttaatttac gcatattcta ta
//
LOCUS       DBKEKI010000203         5886 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.205, whole genome shotgun sequence.
ACCESSION   DBKEKI010000203 DBKEKI010000000
VERSION     DBKEKI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.205"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..121
                     /locus_tag="ACUQPX_004018"
     CDS             <1..121
                     /locus_tag="ACUQPX_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9058501.1"
                     /translation="GTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            455..2734
                     /gene="ydeP"
                     /locus_tag="ACUQPX_004019"
     CDS             455..2734
                     /gene="ydeP"
                     /locus_tag="ACUQPX_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HHV9058502.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINTAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2982..3179
                     /gene="safA"
                     /locus_tag="ACUQPX_004020"
     CDS             2982..3179
                     /gene="safA"
                     /locus_tag="ACUQPX_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HHV9058503.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3253..4014
                     /gene="ydeO"
                     /locus_tag="ACUQPX_004021"
     CDS             3253..4014
                     /gene="ydeO"
                     /locus_tag="ACUQPX_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HHV9058504.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSVLAI"
     gene            4416..>5886
                     /locus_tag="ACUQPX_004022"
     CDS             4416..>5886
                     /locus_tag="ACUQPX_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HHV9058505.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDY"
BASE COUNT         1707 a         1280 c         1275 g         1624 t
ORIGIN      
        1 ggggacagta aataaaagca aagtacctct gggtttaagc gcaacctatg gacaaacagg
       61 aaacaaggtt tcagccggaa ccgtccagtc agtcattggt gtcactttta tctatgaata
      121 attgtgattt tgaccttagt ctatgcgctc cattcacgaa aattgcgcgg ataattattt
      181 tgtgaaggct attatcctac acctgtaaga aaatccgcgc acaattgaag ccagagtctt
      241 attttttata ttgaaaaata tgttaattta aacactatga gagatgatgc ttaacttatc
      301 gtatttaatc tattgtttaa cgataataga ttaaaaatag aaagagagta gtcccctact
      361 cttatatatc catgttggcg ataatccctt tgtatgtact gtgcatcatc gctattacaa
      421 atcctaataa ttcatttcca cacaggataa gtagatgaag aaaaaaattg aatcctacca
      481 gggtgctgca ggtggttggg gtgctgttaa atccgtagcg aatgcagtac gtaagcagat
      541 ggatatacgc catgatgtta ttgccatgtt tgacatgaat aagccagagg gctttgactg
      601 tccgggttgt gcatggccag atcctaagca cagtgcgtca ttcgacattt gtgaaaacgg
      661 cgcaaaagca atcgcctggg aagtcacgga taagcaggta aatgcctctt tctttgctga
      721 gaatacggtt caatcattac ttacctgggg agatcacgag cttgaggctg cggggcgtct
      781 cactcagcct ttgaaatatg atgccgtcag cgactgttac aagccattaa gctggcaaca
      841 agctttcgac gaaattggcg cacgccttca aagctatagt gatcccaatc aggttgaatt
      901 ctatacttcg ggccgcactt ccaatgaagc tgcctttctt tatcagcttt ttgcccgtga
      961 atacgggagc aataactttc ccgactgctc caacatgtgc catgaaccga caagcgtggg
     1021 tttggcagcg agtatcggtg taggtaaagg gaccgtgttg ctggaagact ttgagaagtg
     1081 cgatttagtc atttgcattg ggcataaccc tggtacaaac caccctcgca tgctgacttc
     1141 gttgcgcgct ttagtgaaac ggggagcgaa aatgatcgcc atcaatcctc tacaggaacg
     1201 tggcctggag cgatttaccg caccgcaaaa cccgtttgaa atgctgacga actctgagac
     1261 tcagttggcc agtgcctact acaacgtgcg cattggtggc gatatggcgt tgctcaaggg
     1321 gatgatgcgc ctgttaattg agcgcgatga tgctgcaagt gccgcaggtc ggccctcatt
     1381 gcttgatgac gaatttattc aaacgcatac cgtcggcttt gacgagctac gccgtgacgt
     1441 tctcaattcc gagtggaaag atatcgaacg tatttctgga ctaagtcaga cacaaatcgc
     1501 cgaactggct gacgcatatg ccgctgccga acgcaccatt atctgttacg gaatggggat
     1561 cactcagcac gaacatggta cccagaacgt gcagcaactg gtcaatttgc tgttgatgaa
     1621 aggtaacatt ggcaagcctg gtgcgggtat ctgcccacta cgtggtcact ctaatgtaca
     1681 gggcgaccga accgtcggta tcaccgagaa accgtctgca gagtttctgg ctcgcctggg
     1741 tgagcgctat ggcttccccc cacctcatgc acctggacat gctgcaattg ccagcatgca
     1801 agcaatatgt acggggcagg ctcgagcatt gatctgcatg gggggcaact ttgcactggc
     1861 aatgccagat cgggaagcga gcgctgtacc gttaacgcaa ttagatttgg cggtacacgt
     1921 agccactaag cttaaccgct ctcatctgct gaccgcacgg catagctata ttctgccggt
     1981 tctggggcgt agcgagattg atatgcaaaa aagcggtgcg caggcggtaa ccgttgagga
     2041 ttcaatgtcg atgattcatg cctcgcgtgg cgtgttaaaa cccgccggtg taatgctgaa
     2101 atcagagtgt gcagtggtcg cgggaatcgc gcaggcagca ctaccccaga gcgtggtagc
     2161 ctgggagtat ctggtggaag attatgatcg cattcgcaat gacattgaag ctgtgctgcc
     2221 agagttcgcc gactataacc agcgcatccg tcatcccggt ggttttcacc tgataaatac
     2281 agctgctgaa aggcgctgga tgacgccgtc aggtaaggct aatttcatta ccagcaaagg
     2341 gctgttagaa gatccctctt cagcgtttaa cagtaagctg gtcatggcga cagtacgcag
     2401 ccacgatcag tacaacacga cgatttatgg tatggatgat cgctatcgag gggtattcgg
     2461 tcaacgagat gtggtcttta tgagtgctaa acaagctaaa atttgccgtg taaaaaacgg
     2521 cgaaagagtt aatcttattg cgcttacgcc agacggtaag cgcagctcac gccgcatgga
     2581 tagattaaaa gtggtcattt accctatggc tgaccgctca ctggtgacct attttccaga
     2641 atcgaatcac atgctaacac ttgataacca cgatccatta agtggcattc ctggctataa
     2701 aagtattccg gttgaattag aaccatcaaa ttaatgcctc ttctcatttc ttctgctgtc
     2761 atccgcacag cagaagaatt cctcattgac tattattgcg caatttgctc acatggatta
     2821 aattaaacta catactataa gatataaact tctgcctaca gctgtaagaa actccgctca
     2881 gtactgaagc accagtccta tttcttcttt tctccagcct gttatattaa gcatactgat
     2941 taacgacttt taacgttatc cgctaaataa acatatttga aatgcatgcg accacagtga
     3001 aaaacaaaat cacgcaaaga gacaactata aagaaatcat gtctgcaatt gtggttgtct
     3061 tattactgac acttacgttg atagccattt tttcggcaat tgatcagctg agtatttcag
     3121 aaatgggtcg cattgcaaga gatcttacac atttcattat caatagtttg caagactgaa
     3181 aataaacagc aaattataaa tgtgaaatat ttaaaaggta tcgataaaac tttattgttt
     3241 taaggagata aaatgtcgct cgtttgttct gttatattta ttcatcatgc tttcaacgct
     3301 aacattttag ataaagatta cgccttctct gacggcgaga tcctgatggt agataacgct
     3361 gttcgtacac attttgaacc ttatgagcgg cattttaaag aaatcggatt taatgaaaat
     3421 accattaaaa aatatctaca atgcactaac atccagacag tgacggtgcc tgttcctgcg
     3481 aagtttttac gtgcttcaaa tgtaccgact ggattgctta atgaaatgat tgcttatctc
     3541 aactcggaag aacgcaatca tcataatttt tcagaacttt tgcttttttc ttgcctgtct
     3601 atttttgccg catgcaaagg tttcattaca ctattaacta acggtgtgct atccgtttcc
     3661 gggaaagtga gaaatattgt caacatgaag ctggcgcacc catggaagct gaaagatatt
     3721 tgtgactgcc tgtacatcag tgaaagcctg ttgaagaaaa aactaaagca agagcaaacg
     3781 acattctcac agattctatt agatgccaga atgcagcacg caaaaaattt gatacgcgta
     3841 gaaggttcag tcaataaaat tgccgaacaa tgtggttatg ccagtacatc ttattttatt
     3901 tatgcgttcc gcaaacattt cggcaacagt ccgaagagag tttctaagga gtaccgttgt
     3961 caaaggcaca cgggtatgaa tacgggcaac acgatgagtg ttttagctat ttgattattt
     4021 gctaacgagt agtcaaccac gcacgctacg taagaattat atggggcagc cattccctgc
     4081 cccgcgttgt ttttaggcga tatatttatt gaaataaata agtgacatcc atcacatatt
     4141 tatgcacttg cataacctgt tgcatgatta tttatgatct caattcggca ttttgtcagt
     4201 aaaatgcaat aatttattaa atatcaataa attagttgtt tatcggcgag aaattactta
     4261 atagaacaga aagtaatgtc aacgctttat ggactgttat ttcccttttt ttagctaaat
     4321 ctgctatctc tttatgtgac taacttcact tacatccact tatttctctt cgtaaaatta
     4381 cttcggaatt aagtacaata agaagaggaa catttatgaa gtctgcatta aagaaaagtg
     4441 tcgtaagtac ctcgatatct ttgatactgg catctggtat ggctgcattt gctgctcatg
     4501 cggcagatga tgtaaagctg aaagcaacca aaacaaacgt tgctttctca gactttacgc
     4561 cgactgaata cagtaccaaa ggaaagccaa atattatcgt actaaccatg gatgatcttg
     4621 gttatggaca acttcctttt gataagggat cttttgaccc aaaaacaatg gaaaatcgtg
     4681 aagttgtcga tacctacaaa atagggatag ataaagccat tgaagctgca caaaaatcaa
     4741 cgccgacgct cctttcatta atggatgaag gcgtacgttt tactaacggc tatgtggcac
     4801 acggtgtttc cggcccctcc cgtgccgcaa taatgaccgg tcgagctccc gcccgctttg
     4861 gtgtctattc caataccgat gctcaggatg gtattccgct aacagaaact ttcttgcctg
     4921 aattattcca gaatcatggt tattacactg cagcagtagg taaatggcac ttgtcaaaaa
     4981 tcagtaatgt gccggtaccg gaagataaac aaacgcgtga ctatcatgac aacttcacca
     5041 cattttctgc ggaagaatgg caacctcaaa accgtggctt tgattacttt atgggattcc
     5101 acgctgcagg aacggcatat tacaactccc cttcactgtt caaaaatcgt gaacgtgtcc
     5161 ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc aattggcgtt gttgatcgtg
     5221 ccaaaacact tgaccagcct tttatgcttt acctggctta taatgctccg cacctaccaa
     5281 atgataatcc tgcaccggat caatatcaga agcaatttaa taccggtagt caaacagcag
     5341 ataactacta cgcttccgtt tattctgttg atcagggtgt aaaacgcatt ctcgaacaac
     5401 tgaagaaaaa cggacagtat gacaatacaa ttattctctt tacctccgat aatggtgcgg
     5461 ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg ctataagagt cagacctatc
     5521 ctggcggtac tcacacccca atgtttatgt ggtggaaagg aaaacttcaa cccggtaatt
     5581 atgacaagct gatttccgca atggatttct acccaacagc tcttgatgca gccgatatca
     5641 gcattccaaa agaccttaag ctggatggtg tttccttgct gccctggttg caagataaga
     5701 aacaaggcga gccacataaa aatctgacct ggataacctc ttattctcac tggtttgatg
     5761 aggaaaatat tccattctgg gataattacc acaaatttgt tcgccatcag tcagacgatt
     5821 acccgcataa ccccaacact gaggacttaa gccaattctc ttatacggtg agaaataacg
     5881 attatt
//
LOCUS       DBKEKI010000204         5876 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.206, whole genome shotgun sequence.
ACCESSION   DBKEKI010000204 DBKEKI010000000
VERSION     DBKEKI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.206"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1247
                     /locus_tag="ACUQPX_004023"
     CDS             <1..1247
                     /locus_tag="ACUQPX_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HHV9058506.1"
                     /translation="MGRYFKDAGYHTCYIGKWHLDGHDYFGTGECPPEWDADYWFDGA
                     NYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNRAVDFLQQPVRAEEPFL
                     MVVSYDEPHHPFTCPVEYLEKYADFYYELGEKSQDDLANKPEHHRLWAQAMPSPVGDD
                     GLYHHPLYFACNDFVDDQIGRVINALTAEQRENTWVIYTSDHGEMMGAHKLISKGAAM
                     YDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEKPEILPGENILAVKEPR
                     GVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDELYDRRNDPNEMHNLIDD
                     IRFADVRSKMHDALLDYMDKIRDPFRSYQWSLRPWRKDARPRWMGAFRPRPQDGYSPV
                     VRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(1294..1707)
                     /gene="yidI"
                     /locus_tag="ACUQPX_004024"
     CDS             complement(1294..1707)
                     /gene="yidI"
                     /locus_tag="ACUQPX_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HHV9058507.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            1816..2163
                     /locus_tag="ACUQPX_004025"
     CDS             1816..2163
                     /locus_tag="ACUQPX_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HHV9058508.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            2153..2515
                     /locus_tag="ACUQPX_004026"
     CDS             2153..2515
                     /locus_tag="ACUQPX_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HHV9058509.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            2512..3009
                     /locus_tag="ACUQPX_004027"
     CDS             2512..3009
                     /locus_tag="ACUQPX_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HHV9058510.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(3017..4201)
                     /gene="emrD"
                     /locus_tag="ACUQPX_004028"
     CDS             complement(3017..4201)
                     /gene="emrD"
                     /locus_tag="ACUQPX_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HHV9058511.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVRTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            4283..4357
                     /gene="ysdE"
                     /locus_tag="ACUQPX_004029"
     CDS             4283..4357
                     /gene="ysdE"
                     /locus_tag="ACUQPX_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HHV9058512.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(4481..4570)
                     /gene="tisB"
                     /locus_tag="ACUQPX_004030"
     CDS             complement(4481..4570)
                     /gene="tisB"
                     /locus_tag="ACUQPX_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HHV9058513.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            5135..5233
                     /gene="ivbL"
                     /locus_tag="ACUQPX_004031"
     CDS             5135..5233
                     /gene="ivbL"
                     /locus_tag="ACUQPX_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HHV9058514.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            5339..>5876
                     /locus_tag="ACUQPX_004032"
     CDS             5339..>5876
                     /locus_tag="ACUQPX_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HHV9058515.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVF"
BASE COUNT         1489 a         1547 c         1528 g         1312 t
ORIGIN      
        1 ctatggggcg ctactttaag gatgccggct atcacacctg ttacatcggc aaatggcatc
       61 tcgacggtca tgactatttc ggcactggcg agtgtccgcc ggagtgggat gctgattact
      121 ggtttgatgg ggcgaactat cttagcgaac tgacggaaaa agagatcagc ctgtggcgca
      181 atggcctaaa cagcgtcgaa gatttacagg cgaaccatat tgacgaaacc ttcacctggg
      241 cgcaccgcat cagcaatcga gcggtagatt ttctgcaaca gcccgtgcgc gccgaggaac
      301 ccttcctgat ggtggtttcg tatgatgagc cgcatcaccc gttcacctgt ccggtggagt
      361 atttagagaa atacgctgat ttttactacg aacttggcga gaaatcacag gatgacctgg
      421 cgaacaaacc ggaacatcac cgcttatggg cgcaggcgat gccatcgcca gtcggtgatg
      481 acgggcttta tcaccatccg ctctattttg cctgcaatga ctttgttgat gaccaaatcg
      541 gacgtgtcat caatgcctta acggcagagc aacgtgaaaa tacgtgggtc atttatacct
      601 ccgatcacgg tgaaatgatg ggcgcacata agctgatcag taaaggagcg gcgatgtatg
      661 acgacatcac ccgtattccg ctgatcatcc gttcgccgca aggggagcgg cgacaggtcg
      721 atacgccagt cagtcatatt gatttactgc cgacaatgat ggcgctggca gatattgaaa
      781 aaccagagat tctgccgggg gaaaatatcc ttgccgtgaa agagccacgc ggcgtgatgg
      841 tggaatttaa ccgctacgag attgagcatg atagctttgg cggttttatt ccggtgcgtt
      901 gctgggtgac ggatgacttt aaactggtgc tcaacctctt caccagtgat gaactttacg
      961 atcgccgtaa tgacccaaat gaaatgcata acctgatcga tgatatccgt tttgcagacg
     1021 ttcgcagcaa aatgcatgac gccttattgg attacatgga caaaattcgc gatccgttcc
     1081 gcagttacca atggagtctg cgtccgtggc gtaaagatgc acgaccgcgc tggatggggg
     1141 cgtttcgccc acgcccacaa gatggctatt cgccagtggt acgcgactat gacaccggcc
     1201 taccgacgca aggagtgaag gtggaggaga aaaaacagaa attctgacga taatgcccgc
     1261 tgatgatcac ccggcgggca ttattcaggc tgcttaaacc agcgaaccgc cggtcaggaa
     1321 ggaaaaaatc aggaatatca gcaaaatgct gcacacgatg ccgataccaa aaaccagcgc
     1381 gccgcgaatg ccccagcgat tttccttacg cataccgcca ataaatgcgc aaagtagcgc
     1441 agcaatggcc agtgcgatac cgctttgatc gaggatttta tcgatatcca tgtcctcttt
     1501 tttctccaca gtggtaattt tttcgccttt tattccggcg agaagacctc ttttaatttc
     1561 agccgttttg tccgccacga tgctttcaag cgttggcggt ggtgcggaaa aagggccgaa
     1621 tgaaaagtgg ataatcccca tcattaaagc gatggcaccg aacagcatgc ccaatgaact
     1681 gattttattt tgcgctatga tacccatctg acctccttgt agcggatggc aaaagcatat
     1741 caggaaacgg gcggacgaaa ttcttaaaaa aaaccgctaa agatagagcg ttagtgaaat
     1801 tgcaaaggag caacaatgaa aatttcccgc cttggagaag cgcccgatta ccgcttctcg
     1861 ctggctaatg agcgtacttt tctggcgtgg atccgtaccg cactaggatt tctggcggcg
     1921 ggggttgggc ttgatcaact tgcgccagat ttcgccacgc ccgtcattcg tgaactgctg
     1981 gcgttgttgt tgtgtctgtt ttccggcggg ttagcaatgt atggctacct gcgctggtta
     2041 cgcaatgaaa aagcgatgcg tctgaaagag gacctgccct acaccaacag cttattaatc
     2101 atcagcttaa tattgatggt tgtggcggtg atcgttatgg gactggtgtt gtatgccgga
     2161 tagccgtaaa gccagacgca ttgccgaccc agggctgcag ccggagcgca catcactggc
     2221 gtggtttcgt accatgctgg gctacggcgc gttgatggcg ttggctatca aacacaactg
     2281 gcaccaggcg ggcatgttat tctggatttc gattggcatc ctcgccatcg tggcgctgat
     2341 cctctggcac tacacccgta atcgcaattt aatggatgtc acgaatagcg atttttccca
     2401 atttcacgta gtccgtgaca aatttttgat ctccctcgcg gtgttatctc tcgcaatact
     2461 gtttgctgta acgcatatac atcaacttat cgtatttatt gagagagtcg catgacagga
     2521 agtcaggtca ttgatgctga agaggacagg cataagctgg ttgtagagta caaggatgct
     2581 ctgcaacctg ccgattttta tcacaatttc aaacagcggg gcatccgctc cgtacagctc
     2641 attccccatc ttgaatttga tgaccggggc gatctgacgg ccgcttcggt gacagcagag
     2701 ctgtggggga aatttttaat tgccctgttt gagtgttggg tgcgggcaga tatcagtcgt
     2761 atctcgattg agctttttga cgccacctta caaaaatggt gtggcagcga aaacccgcaa
     2821 ccgcgccgtg actgccaggc gtgcgactgg catcgcctgt gcccgcacgc tcggcaggag
     2881 acgccagaca gcgtgctttg tgcgggctat caggcgtttt attcttactc cgcaccgcat
     2941 atgcgggtga tgcgtgatct gataaagcag catcgttctc ccatggagct gatgacgatg
     3001 ctgcggtgac gtgcgcttaa acgggctgcc cctgatgcga catccgcgtc gccagcggca
     3061 gccagcacag cacgatcaac aatcccatta aggtcatcaa caaccccagg ctgccctgac
     3121 cggtttgcgg caacatcgca gagagcgacg ccagcacgcc ggaaccaatg ttttgcagac
     3181 cgccgaccag cgcaccagcc gtgcccgcca ggaaggggaa cggctccatc gcgccgctgg
     3241 tcgccagcgg aaacagcatc ccggcaccga aaaagaacag cgcggcggga acgagcagcg
     3301 tcctgacatt catcacgcca aaccagtcgg ggatccacat cagcaagcca gccagcaggc
     3361 agcagataac cgactgccac atcaacgttg agaagcgttt attaggacgc ccggcaaacc
     3421 atgccccaaa aaatgccgcc ggaatcggca gaataaacaa aatactgacc gtcatactgc
     3481 tcagccctaa caccgcgccc atcagcacgc cggagcaggc ttcaaaggca gcaatcccgg
     3541 ccagaccgcc aatcagcatc agcaaataac agttaaaacc gctgttaccg aaaagcgttt
     3601 tataactggt aagcaggcgc gtgcgcggtg catcgaccgg acgcgtttcc ggcatccagc
     3661 gggccatact gaaggtcaca ccagcacaaa gaaccaacaa aaagagataa caggcgcgcc
     3721 agttccacat cgtatccagc agaccgccga ttagcggtgc gagcaacgga ctgacgagaa
     3781 tccccatgtt taacaggctg ttagcatggc gcaactgtgt ccgttcatat aaatcacgcg
     3841 gcaaagtacg cgccattacg ccgccaacgc cggtgcccat cccctgcatc gcgctggcgg
     3901 caatcaatac cgtcaaactg gaggtcgtga ccgcgaccag cgttgccagc ataaaaatgg
     3961 acattccgac gaggatcact ggtcggcgac cgacacggtc ggatatcggg ccataaaaca
     4021 gctgtgagac accgtaagtc agcagataag cgcccattac gctctgcacc gccccttcac
     4081 ggacgttgag atcgcgcgcc atatcggcaa tagctggaat ataaatggtt tgcgccatct
     4141 gaccgacggc cacgagtaat accaacatca ataacaaatt gacgtttctt tgccttttca
     4201 ttatcacgga tgcttttata aaaataatta gaataagtca ctacgcattc ccataaatgc
     4261 gggagaggaa tctatcacat tgatgaatgt tagccagata tacgccagga atggggaatt
     4321 gtttagcggc agaatatgta aacaaaagcg gcaataaatg ttgccgggag ggatggtagg
     4381 gcggataaga tgcgtcagca tcgcatccga ctccaacccg cacgctaaat ataaaagggg
     4441 agcggttacc cgctcccctt tggtgcgact tgaatctgaa ttacttcagg tatttcagaa
     4501 cagcatcaag cagttgcagt gctgcaacaa tgagtttgag gataagaatg gcgatatcca
     4561 ccaggctcat acgcgtctcc tgtggttcag gagcactgcc agctgacgta cctttccgct
     4621 gcctgttgcc agctgttgtg cacgcggtat ctcaaggaga aacgttgtgc ttgttgacat
     4681 aacacagtgt gctcacgcgg ggttaaggcg ctgacggcac cacccgtttc agccaggact
     4741 tagtgcgccg ggtaacgatg cggcccccgc ataacacatt gcgtacagtg atattataga
     4801 agtttactgt ataaataaac agtaatattt ggacaaaacg caaactgtga tcgatactca
     4861 actatgcact aaatacgtca aaattcgtgc cgaaattgcg cgttctgcgc ggaacacgta
     4921 tactttcagt gttgacataa tacagtgtgc tttgcggtta ccagccgcag gcggctgacg
     4981 aaacctcgct ccggcggggt tttttgttat ctgcaattca gtacaaaacg tgatcaaccc
     5041 ctcaattttc ccttgctgaa aaattttcca ttgtctcccc tgtaaagctg tgcttgtata
     5101 aatattgtta aacacaaaac caacaaggtc cccaatgact acttccatgt tcaacgcaaa
     5161 actactacca actgcgccat ccgccgcagt ggtcgtcgtg cgtgtggtgg tggtcgtcgg
     5221 caatgcgccg tagggactgg aacaacacac gattccaaaa ccccgccggc gcaaaccggg
     5281 cggggttttt cgtttaagca cctcccggaa agtcggccca gaagaaaagg actggagcat
     5341 ggcaagttcg ggcacaacat cgacgcgtaa gcgctttacc ggcgcagaat ttatcgttca
     5401 tttcctggaa cagcagggca ttaagattgt gacgggcatt ccgggcggtt ctatcctgcc
     5461 tgtttacgac gccttaagcc aaagcacgca aatccgccat attctggctc gccatgaaca
     5521 gggcgcgggg tttatcgctc agggaatggc gcgcaccgat ggtaaaccgg cggtctgtat
     5581 ggcctgtagc gggccgggtg cgactaacct ggtgaccgcc attgctgatg cgcggctgga
     5641 ctccatcccg ctgatttgca tcactggtca ggttcccgcc tcgatgatcg gcaccgacgc
     5701 cttccaggaa gtggacacct acggcatctc tatccccatc accaaacaca actatctggt
     5761 cagacatatc gaagagctcc cacaggtcat gagcgatgcc ttccgcattg cgcaatcagg
     5821 ccgccctggc ccggtgtgga tagacattcc taaggatgta caaacggcgg tttttg
//
LOCUS       DBKEKI010000205         5845 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.207, whole genome shotgun sequence.
ACCESSION   DBKEKI010000205 DBKEKI010000000
VERSION     DBKEKI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.207"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..873
                     /locus_tag="ACUQPX_004033"
                     /pseudo
     CDS             1..873
                     /locus_tag="ACUQPX_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
     gene            1056..3236
                     /locus_tag="ACUQPX_004034"
     CDS             1056..3236
                     /locus_tag="ACUQPX_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HHV9058516.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(3377..4660)
                     /locus_tag="ACUQPX_004035"
     CDS             complement(3377..4660)
                     /locus_tag="ACUQPX_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HHV9058517.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4794..>5845)
                     /locus_tag="ACUQPX_004036"
     CDS             complement(4794..>5845)
                     /locus_tag="ACUQPX_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9058518.1"
                     /translation="ERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIAITEAIQA
                     NIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAIRR
                     GLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPVAA
                     TRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIV
                     DGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSG
                     TVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFSQHLLGHKSDTMASQ
                     YRDDRGREWDKIEIK"
BASE COUNT         1387 a         1412 c         1490 g         1556 t
ORIGIN      
        1 aaaaccacca gctatatgtt ctttaccgca atggagggga acattctggc gctgaaaatg
       61 atcaacgaca ttctgcacct gcaaattagc tggggtggat gggcgctggc cgccggattg
      121 ccgggcatca ttatgctgct ggtcaccccg ctggtaattt acaccatgta tccaccagaa
      181 attaaaaagg tggataacaa aaccatcgct aaagcgggcc ttgcagaact gggaccgatg
      241 aaaatccgcg aaaaaatgct gctcggtgtc tttgtgctgg cgctactggg ctggattttc
      301 agtaagtctc tgggggttga tgaatccact gtggcaatcg ttgttatggc gaccatgctg
      361 ctgctgggta tcgttacctg ggaagacgtg gttaaaaata aaggcggctg gaatacctta
      421 atctggtacg gcggtattat cggcttaagc tccttattat cgaaagttaa atttttcgaa
      481 tggttagctg aagtctttaa aaataacctg gcatttgatg gtcacggtaa cgttgctttc
      541 ttcgttatta ttttcctcag catcatcgtg cgttatttct tcgcttccgg tagtgcctat
      601 atcgttgcta tgttacctgt atttgccatg ctggcgaacg tctccggcgc accgttaatg
      661 ttaaccgcgc tggcactgtt gttctccaac tcctatggcg gcatggttac tcactatggc
      721 ggcgcggcag gtccggtcat ctttggcgtg ggttacaacg atattaaatc ctgatggttg
      781 gtcggtgcgg tactgacgat attaaccttc ctggtgcata tcaccctcgg cgtgtggtgg
      841 tggaatatgc tgatcggctg gaacatgctg taaatatact cgtcatactt caagttgcat
      901 gtgctgcgtc tgcgttcgct caccccagtc acttacttat gtaagctctt ggggattcac
      961 tcacttgtcg ccttcctgca actcgaatta tttagagtat atccatttat tatctttctg
     1021 cgcacttcac ggtgcgcaga tatctggagc atttgatgat caagttatct gaaaaaggcg
     1081 tgtttctcgc cagtaataac gaaataattg ccgaagaaca tttcaccggc gaaattaaaa
     1141 aagaagaagc aaaaaaaggc actattgcct ggtctattct ctcttctcat aatacgtccg
     1201 gaaatatgga taaacttaaa attaagtttg attcattagc ctctcacgat attacctttg
     1261 ttggtattgt acagaccgct aaagcgtccg gaatggaacg tttcccgctg ccgtatgtgc
     1321 tgaccaactg ccataactca ctctgcgccg tcggcggcac cattaacggt gatgaccatg
     1381 tttttggttt atcggcagct cagcgttatg gcggtatttt tgtgcctccg catattgcgg
     1441 tcatccatca atatatgcgt gagatgatgg caggcggggg caaaatgatc ctcgggtcag
     1501 acagccacac ccgttacggt gcattaggga caatggcagt cggtgagggt ggcggtgagt
     1561 tggtaaaaca gctgcttaat gacacctggg atatcgacta tccgggcgtg gttgcggtgc
     1621 atctgaccgg aaaacctgcg ccgtatgtgg ggccacagga tgtggcgctg gctatcattg
     1681 gcgcggtgtt caaaaacggt tacgtcaaaa acaaagtcat ggagttcgtt ggaccgggcg
     1741 ttagcgcgct ctctaccgat ttccgtaaca gcgttgacgt gatgaccacg gaaaccacct
     1801 gtttaagctc cgtctggcaa actgatgaag aagtgcataa ctggctggcg ctgcacggtc
     1861 gcggccagga ttactgccag cttaaccctc aaccgatggc gtactacgat ggctgcatca
     1921 gcgttgattt aagcgccatc aaaccaatga ttgcgctgcc gttccacccg agcaacgtgt
     1981 atgaaatcga cacactgaac cagaacctga ccgacattct gcgtgagatt gaaattgagt
     2041 ccgaacgcgt ggcgcacggt aaagccaaac tctcgctgct ggataaagtg gaaaatggtc
     2101 gcctgaaagt gcagcagggg attatcgcgg gctgttctgg cggtaactac gaaaacgtca
     2161 tcgcggcggc gaatgcactg cgcggtcaat cctgtggcaa tgacaccttc tcgctggcgg
     2221 tttacccgtc atcacagccg gtgtttatgg atctcgccaa aaaaggtgtg gtagcagatt
     2281 tgattggcgc aggcgcaatc atcagaaccg cattctgtgg cccatgcttt ggcgcgggcg
     2341 atacgccaat caacaacggt ttgagtattc gccacaccac gcgtaacttc ccgaaccgcg
     2401 aaggctctaa gccagccaat gggcagatgt cagcggtggc gttgatggac gctcgttcta
     2461 tcgctgcgac tgcggcaaac ggtggctatt taacctctgc cagcgaactt gattgctggg
     2521 acaacgtgcc ggagtacgcc ttcgatgtaa cgccgtataa aaaccgtgtt tatcagggct
     2581 ttgtgaaagg ggcaactcag caaccgctga tttacggacc gaacattaaa gactggccgg
     2641 aattgggtgc gctgactgac aatatcgtcc tgaaagtgtg ctcgaagatc ctcgacgaag
     2701 tcaccaccac cgacgaactg attccttccg gtgaaacctc ttcttatcgt tcaaatccga
     2761 ttggtctggc ggggaatgtc agcgagctga cagaggtgtt tgcgcgcatt aagcagattg
     2821 ctggtcagga gcatattgat ccgctgcaaa ctgaaattgg cagcatggtc tatgcggtga
     2881 aaccaggcga tggttctgcg cgtgaacagg cggcgagctg ccagcgtgtg attggcggtc
     2941 tggcgaatat tgccgaggag tacgcgacta aacgctaccg ttctaacgtc atcaactggg
     3001 ggatgttacc gctgcagatg gcggaagtgc caacctttga agtgggggat tacatttaca
     3061 tccctggcat taaagcggcg ctggataatc cgggtacgac gtttaaaggt tatgtgatcc
     3121 atgaagatgc gccggtaacg gaaattacgc tttacatgga aagcctgacg gctgaagagc
     3181 gcgagattat caaggcgggt agtttgatta acttcaataa aaaccgtcag atgtaaaaag
     3241 cgccatgtga atgtaggtcg cattcggcac ttattgtcgg atgcgatgct ggcgcatctt
     3301 atccgaccta caaatcgcat cgaaccgtag gccggataag gcgtttacgc cgcatccggc
     3361 aaatagttaa tagctcttac ttcttcgcct ctgcaaccac tttgctgccc acgccgcggt
     3421 tattgtattc ccacatgcgg ttgtagttag tgtcattcag attgcgctgt atttcgtcgt
     3481 tatcatctac gctgccggta ttacccgcaa acggacgatt agagatcacc gcatcggccc
     3541 acggtttagc cgtgttaaaa ccttcgttga tggcgctatc acggatcacc acctgaccgt
     3601 tggtattagc atcaacatcc agcgagcggc ccagttgcgc cacgccatca ccggaagcat
     3661 tgaaacggct gtttacggcg aggaaaccgt aatagatgtt agacagcgta gccggtgcaa
     3721 acacatatgc ttcttgctgg gtacgtgagt tcaccacgcg gaattcggtg ttatcgaaca
     3781 ccactgcgcc gcgaccagaa acgatatcca catccccttc aatatagctg ttggtcacca
     3841 gcgtacgcgg ctgacggttg gtttccagac ggttctgcac accgctgttg gtgacaaaga
     3901 aggtgttctg acgaccgaga atgttaacgt tgttaatctg tacctggtca ccgtcagtac
     3961 gcagtgctac cgccggatgg ttacctgcat ctacgctatc gcccagcgtg ttttcgatgg
     4021 tcagattttg cagttgcagt ccattgtttt gtgaccagaa gaccgcagaa cacagaacac
     4081 cgatactgtc gctgcgtttg ctctggcagc tatcgtacat ataccacgct ggtttacctg
     4141 gcatatattt gccgcgcggg ttgacgtcgt gacgccagtc ggcagggctc atgcccccat
     4201 caagggaaag cccaatcttc acatcaatcg gtttttcacc tgtaccgtac agagtgattc
     4261 cacctggagc agcagggaca tacaccgttc cctgatactc accaggcatc acggcaatat
     4321 actggcgctt gttggtgcgc ttgataattg ccgcatctac cgccgcctga atcgtggtat
     4381 gcgttacacc ttgagtaccc gccgggccga caacaaagtc aggttgcgca ggcagggtta
     4441 tcggggacgg attccacgct gcagcacctg gtgtcaggga tgcaaaatag tgttgcgcat
     4501 cgaaattcag cgcttctttt gccgacagaa tcgggcgaga agaggtacca ggcgcagttt
     4561 gatcagaagg acgttgatcg ggtggtgttg agctacaggc ggtcagcgtc acgccaaaag
     4621 ccaatgccag cgccagacgg gaaactgaaa atgtgttcac aggttgctcc gggctatgaa
     4681 atagaaaaat gaatccgttg aagcctgctt tttatactaa gttggcatta taaaaaagca
     4741 ttgcttatca atttgttgca acgaacaggt cattatcagt caaaataaaa tcattatttg
     4801 atttcaattt tgtcccactc cctgcctctg tcatcacgat actgtgatgc catggtgtcc
     4861 gacttgtgcc cgagaagatg ttgagaaaac ttatcgctta tctgcttctc atagagtctt
     4921 gcagacaaac tgcgcaactc gtgaaaggta ggtggatccc cttcgaagga aagacctgat
     4981 gcttttcgtg cgcgcataaa ataccttgat actgtgccgg atgaaagcgg ttcacgacga
     5041 gtagatgcaa ttatggtttc tccgccaaga atctctttgc atttatcaag tgtttccttc
     5101 attgatatcc cgagagcatc aacatgcaat gttgtaggga tggcaatttt tacgcctgtt
     5161 ttgctttgct cgacataaag atatccatct acgatatcag accacttcat ttcgcataaa
     5221 tcaccaactc gttgcccggt aacaacagcc agttccattg caagtctgag ccaacatggt
     5281 gatgattctg ctgcttgata aattttcagg tattcgtcag ccgtaagtct tgatctcctt
     5341 acctctgatt ttgctgcgcg agtggcagcg accgggtttg ttgttatatg gccttcagct
     5401 attgcctctc ggaatgcatc gctcagtgtt gatctgatta acttggctga tgccgccttg
     5461 ccctcgtcta tgtatccatt gagcattgcc gcaatttctt ttgtggtgat gtcttcaagt
     5521 ggagcatcag gcagccccct ccttattgct ttaattttgc tcatgtaatt tatgagtgtc
     5581 ttctgcttga ttcctctgct ggcgaggatt ttttcgtagc gatcaagcca tgaatgtaac
     5641 gtaacagaat tatcactgtt gattctcgct gtcagaggct tgtgtttgtg tcctgaaaat
     5701 aactcaatgt tggcctgtat agcttcagtg attgcgattc gcctgtctcg gcctaatcca
     5761 aactctttac ccgtccttgg gtccctgtag cagtaatatc cattgtttct tatataaagg
     5821 ttagggggta aatcccggcg ctcat
//
LOCUS       DBKEKI010000206         5794 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.208, whole genome shotgun sequence.
ACCESSION   DBKEKI010000206 DBKEKI010000000
VERSION     DBKEKI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.208"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            221..310
                     /gene="kdpF"
                     /locus_tag="ACUQPX_004037"
     CDS             221..310
                     /gene="kdpF"
                     /locus_tag="ACUQPX_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HHV9058519.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            310..1983
                     /gene="kdpA"
                     /locus_tag="ACUQPX_004038"
     CDS             310..1983
                     /gene="kdpA"
                     /locus_tag="ACUQPX_004038"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HHV9058520.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAELFFMLLGQHYLPRNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2006..3756
                     /gene="kdpB"
                     /locus_tag="ACUQPX_004039"
                     /pseudo
     CDS             2006..3756
                     /gene="kdpB"
                     /locus_tag="ACUQPX_004039"
                     /inference="COORDINATES: protein motif:HMM:NF012905.6"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4062..4634
                     /gene="kdpC"
                     /locus_tag="ACUQPX_004040"
     CDS             4062..4634
                     /gene="kdpC"
                     /locus_tag="ACUQPX_004040"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HHV9058521.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4627..>5794
                     /locus_tag="ACUQPX_004041"
     CDS             4627..>5794
                     /locus_tag="ACUQPX_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="sensory histidine kinase in two-component
                     regulatory system with KdpE; signal sensing protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HHV9058522.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFSACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSR"
BASE COUNT         1200 a         1516 c         1700 g         1378 t
ORIGIN      
        1 atcgggcatt atctgaacat aaaacactat cagtaagttg gagtcattac ccacactgta
       61 attaaattac acaaaagtta aattaatact aaccatcagt taaatcatgg cctttgccat
      121 ttttatactt tttttacacc ccgtccgcag atttttgcga aatctttgca gccagaattc
      181 tactcttccg gtatcacttt taggccactg gaggtgcact gtgagtgcag gcgtgatagc
      241 cggcgtgttg ctggtgtttt tattactggg ttatttggtt tatgccctga tcaatgcgga
      301 ggcgttctga tggctgcgca agggttctta ctgattgcca cgtttttact ggtattaatg
      361 gtgctggcgc gtcctttagg cagcgggctg gcgcggctga ttaatgacat tcctcttcca
      421 ggtacagcgg gcgttgaacg tatactcttt cgtttacctg gcgtctctga ccatgagatg
      481 aactggaagc aatatctttg tgccattctc ggcctgaaca tgctggggct ggcggagctg
      541 ttttttatgt tgctcggtca gcactatctg ccgcgtaatc cacagcagtt gccggggctg
      601 tcgtgggatc tggcgctgaa taccgccgtc agctttgtca cgaataccaa ctggcaatct
      661 tatagcggtg aaaccacgtt gagctatttc agccagatgg caggcttaac ggtacaaaac
      721 tttctttctg ccgccagcgg gattgcggtg atttttgcct tcatccgtgc gtttactcgc
      781 cagagcatga gcacactcgg gaatgcctgg gtcgatctgc tacgcatcac gttatgggtg
      841 ctagtccctg tggcgttgtt gatcgcactg ttttttattc aacaaggtgc gctgcaaaac
      901 tttctgccct atcaggcagt gaataccgtt gaaggagcgc aacagctgtt acccatggga
      961 ccagtagctt ctcaagaagc gatcaagatg ctcggtacta acggcggtgg cttctttaat
     1021 gccaactcgt cgcatccgtt tgaaaaccca accgcactga ccaacttcgt gcagatgctg
     1081 gcgatcttct tgatcccaac agcgctgtgc tttgcctttg gtgaagtgac gggcgatcgc
     1141 cgccaggggc gcatgttgct gtgggcgatg tcagtgattt ttgtcatctg cgtaggcgtg
     1201 gttatgtggg cagaagttca gggtaatcct catctgctgg cgctcggcgc agacagcagc
     1261 atcaatatgg aaggtaaaga gagccgtttc ggcgtgctgg tcagtagcct gtttgcggtc
     1321 gtgactacgg cggcgtcctg tggcgcggtg attgcgatgc atgattcgtt taccgctctc
     1381 ggcggcatgg tgccgatgtg gctaatgcaa attggtgaag tggtgttcgg cggtgtcggt
     1441 tctggtcttt acggcatgat gctgtttgtc ctgctggcgg tgtttattgc cgggctgatg
     1501 attggtcgta caccggaata tctgggtaaa aaaatcgacg tacgcgagat gaaactgact
     1561 gcactggcga ttctggttac tccaacactg gtactgatgg gcgcggcgtt ggcgatgatg
     1621 accgacgccg gacgtagcgc catgcttaac cctggcccgc atggttttag cgaagtgctg
     1681 tacgccgtgt cgtccgccgc taacaacaac ggcagcgcct ttgccggatt aagcgccaac
     1741 tctccgttct ggaactgttt actggcgttc tgcatgtttg tcggtcgctt cggggtgatt
     1801 atcccggtga tggcaattgc cggttcgctg gtgagtaaaa agagccaacc cgccagctcc
     1861 ggcacgctgc caacgcacgg cccgctgttt gttggcctgt taatcggcac cgtgttgctg
     1921 gttggcgcac tgacctttat ccctgccctg gcgcttggtc cggtggcgga atatctctcc
     1981 tgatgatatt gagtgagcac tgaatatgag tcgtaaacaa ctggcgctat tcgaaccaac
     2041 acttgtcgtt caggcgctga aagaagcggt gaaaaaatta aacccgcagg cgcaatggcg
     2101 caatccggtg atgtttatcg tctggatcgg cagtctgctg accacctgta ttagcatcgc
     2161 gatggcaagc ggtgcgatgc ctggcaatgc gctgtttagc gcggcaatta gcggctggct
     2221 gtgggtcacc gtactgttcg ctaatttcgc cgaggcgctg gcagaaggcc gcagtaaagc
     2281 gcaggccaac agtcttaaag gggtgaaaaa aactgccttt gcccgcaagc tgcgtgagcc
     2341 gaaatatggc gctgcggcgg acaaagttcc tgccgatcaa ctgcgtaaag gtgacatcgt
     2401 gctggtagaa gctggcgata ttatcccctg cgatggtgaa gtcattgaag gcggcgcatc
     2461 ggtcgatgaa agcgccatca ccggggaatc ggcaccggtg atccgtgaat ccggcggcga
     2521 ttttgcctcc gtcaccggcg gcacgcgtat tctttctgac tggctggtga ttgagtgtag
     2581 cgttaacccc ggtgagacat ttctggatcg gatgatcgcg atggtggaag gcgcacagcg
     2641 acgcaaaaca ccgaacgaga ttgccctgac tatcctgctg attgctctga ccatcgtctt
     2701 tttactggca accgccacgc tgtggccgtt ttccgcgtgg ggcggtaatg ccgtgagtgt
     2761 aacggtactg gtggcgctgc tggtctgtct gatcccaacc actattggtg gcctgttgtc
     2821 agcgatcggc gtcgccggga tgagtcggat gctgggcgcg aatgtgattg ccaccagcgg
     2881 acgtgcagtt gaagcggcag gtgacgttga cgttctgcta ctggataaaa ccggcaccat
     2941 cacactcggt aaccgtcagg cgtcggagtt tatccccgcg cagggcgtgg atgaaaaaac
     3001 gctggctgac gccgcacaac tggcttcgct ggctgatgaa acgccggaag gccgcagtat
     3061 tgtgatcctc gccaagcaac gttttaacct gcgcgagcgc gatgtgcagt cgctccatgc
     3121 cacctttgta ccgtttactg cgcaaagccg gatgagcggg atcaacatcg acaaccgcat
     3181 gatccgtaaa ggttctgtcg atgccattcg ccgccatatt gaggctaacg gcggtcactt
     3241 ccctgccgat gttgatcaaa aagtcgatca ggttgcgcgt caaggagcca cgccgctggt
     3301 ggtggtggaa ggttctcgtg tgctgggcgt tattgcgctg aaagatatcg tcaaaggcgg
     3361 tattaaagag cgcttcgccc agctgcgcaa atgggcatta aaacggtgat gattaccggc
     3421 gataaccgtc tgactgccgc cgcgatagct gcggaagcgg gtgtcgatga ttttctcgcc
     3481 gaagcgacac cggaggccaa gctggcattg atccgtcagt atcaggcgga aggtcgtttg
     3541 gtagcgatga ccggcgacgg caccaacgat gctccggcgc tggcgcaggc agatgtcgcg
     3601 gtggcgatga actccggcac ccaggcggcg aaagaggcgg gcaatatggt ggatctcgac
     3661 tctaacccga ccaagttgat cgaggtggtg cacattggca aacagatgct gatgacccgt
     3721 ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg
     3781 gcggcgttcg cggcaacata tccgcagtta aatgcgctga acatcatgcg cctgcattcg
     3841 cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt
     3901 cctctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt
     3961 aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt
     4021 gatttactgc taaccgtttg tagtctggtg tgaggtttac catgagtgga ttacgtccgg
     4081 cattatcgac atttctcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca
     4141 ccgcactggg gcaatggtgg tatccctggc aggccaacgg ttcgttgatt cgtgaaggtg
     4201 atacggtgcg cggttcggca ttaatcgggc aaaattttac cggcaacggc tattttcatg
     4261 gtcgcccgtc ggcaacagca gaaatgccct ataatccaca ggcttctggc gggagcaatc
     4321 tggcagtcag taacccggag ctggataaac agatagccgc acgcgttgcc gcattacggg
     4381 ccgctaaccc ggacgccagc acgaacgttc cggttgaact ggtgacggcg tcggcaagcg
     4441 gactggacaa taacatcacc ccgcaagcgg cggcctggca gatcccacgc atcgcaaaag
     4501 cgcgtaatct cagcgttgaa cagctcacgc agctgatcgc aaaatacagc caacaaccgc
     4561 tggtgaaata tatcggccag ccggtcgtca acattgtcga actcaatctg gcactggata
     4621 agcttgatga ataacgaacc cttacgtccc gaccccgacc gtctgctgga acaaactgcc
     4681 gcgccgcatc gggggaagct gaaagttttc ttcagtgcct gtgcaggcgt cgggaagacc
     4741 tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggt
     4801 gtggtagaaa cccatgggcg gaaagatacc gccgccatgc ttgaagggct ggctgttctg
     4861 cctctgaaac gccaggcgta ccgtgggcgg catatcagcg agtttgatct cgatgccgcc
     4921 ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt
     4981 tctcgtcatc ctaaacgctg gcaggatatc gaagaactgc tggaagctgg cattgatgtt
     5041 ttcacaaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc
     5101 ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtggtg
     5161 ctggtggact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt
     5221 gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg
     5281 cgcgaactgg cactgcgccg tactgccgat cgcgttgatg agcaaatgcg cgcctggcgg
     5341 gggcatcctg gcgaagagaa agtctggcac acgcgcgacg cgatcctttt atgcatcggc
     5401 cataacaccg gcagcgaaaa actggtccgc gcggcggcgc ggctggcgtc acggctgggg
     5461 agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa
     5521 cgtcgggcaa ttctcagcgc cttacgtctg gcgcaggagc tgggcgcgga gacggcaaca
     5581 ctttctgatc cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca taatctcggc
     5641 aagattattc tcggtcgccc agcctcgcgc cgctggtggc gtcgggaaac atttgctgac
     5701 cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc
     5761 gcccgcacga ttaacaacgc gccggatagc cgct
//
LOCUS       DBKEKI010000207         5763 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.209, whole genome shotgun sequence.
ACCESSION   DBKEKI010000207 DBKEKI010000000
VERSION     DBKEKI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.209"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(219..2288)
                     /gene="glyS"
                     /locus_tag="ACUQPX_004042"
     CDS             complement(219..2288)
                     /gene="glyS"
                     /locus_tag="ACUQPX_004042"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HHV9058523.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2298..3209)
                     /gene="glyQ"
                     /locus_tag="ACUQPX_004043"
     CDS             complement(2298..3209)
                     /gene="glyQ"
                     /locus_tag="ACUQPX_004043"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HHV9058524.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3304..3603)
                     /locus_tag="ACUQPX_004044"
     CDS             complement(3304..3603)
                     /locus_tag="ACUQPX_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HHV9058525.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3778..4773
                     /gene="wecH"
                     /locus_tag="ACUQPX_004045"
     CDS             3778..4773
                     /gene="wecH"
                     /locus_tag="ACUQPX_004045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HHV9058526.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4815..5252)
                     /gene="yiaA"
                     /locus_tag="ACUQPX_004046"
     CDS             complement(4815..5252)
                     /gene="yiaA"
                     /locus_tag="ACUQPX_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HHV9058527.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5298..5639)
                     /gene="yiaB"
                     /locus_tag="ACUQPX_004047"
     CDS             complement(5298..5639)
                     /gene="yiaB"
                     /locus_tag="ACUQPX_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HHV9058528.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT         1424 a         1462 c         1392 g         1485 t
ORIGIN      
        1 tgcctttcgg tgaatacctg agacaaacag tctcaagcac ccgtggctat tctagcttaa
       61 taagtttgtt tcttctcctt gatataatcc taaaaaaatc tcataaaatt aatatatgag
      121 ataatcttta ttcagcagaa gattattaaa ggttgctgta ttatttagcg ataataaaaa
      181 aagcctgcca gatggcaggc tttttaataa cggcgttatt actgcaacag cgaaatatcc
      241 gcaacgcgca ggaacagttc gcgcagtttc tccagcatgg tcagacggtt gagacgcaat
      301 tctttgtcat caaccatgac catcacttta tcgaagaaag catcaaccgg ctcacgcagc
      361 tctgccagtt cgaccagcgc atcctggtaa cgaccttccg caaagtacgg ctccagcttg
      421 tcacgtagca ccacaacctg catcgccagt ttaatttctt ccggctcttt cagggtagag
      481 gcattcacgc gatcactcag cacttcgtca gatttcgcca ggatgttaga tacacgtttg
      541 ttcgccgccg ccagtgctgc agctgcttcc agggtacgga agtgcgatac cgctttcatg
      601 cgggcatcga aatcagccgg acgagtcgga cgacgcgcca gtaccgcctg gatggtgtca
      661 acggtataac cttcgtcctg ataccaggcg cggaagcgac cgagcataaa gtcgataaca
      721 tcatcaacta cgttggcatt agtcagctta tcgccataca gacgcaccgc ctcttcggtc
      781 agcgtttgca gatcaaggtt gaggttcttc tcaacgataa ttcgcagcac gccaagtgcg
      841 gcacgacgca gcgcaaacgg gtctttgtcg cctttcggat gctgaccgat accgaagata
      901 cccgccagag tatccatctt atcagcaatc gccagcgcac aagctaccgg gttggacggc
      961 aggtcatcac cggcaaagcg cggctgatac tgctcattca gcgcgacggc tacgtcttcc
     1021 gcttcgccat cgtgacgcgc gtagtgcatc cccataacgc cttgggtgtc ggtgaactcg
     1081 aagaccatgt tggtcatcag gtcacactta gacagcaggc ccgcacgggt tgcgtggtta
     1141 acgtcagcgc caatctgttc ggcgatccag ccagccagcg cctggatgcg gtcagtcttg
     1201 tcacgcagcg tacccagctg ttgctggaac agcacggttt gcaggcgcgg caggttatct
     1261 tccagacgtt ttttgcggtc ggtgttgaag aagaactcgg catccgccag acgtggacga
     1321 acgactttct cgttaccaga gataatttgc tgcggatctt tcgattcgat gttggcaacg
     1381 aagataaagt tcggcagcag tttgccgtcg ttcgcataca ccgggaagta cttctggtca
     1441 cctttcatgg tgtaaaccag ggcttcagcc ggcaccgcga ggaatttctc ttcgaatttt
     1501 gcggtcagaa cgaccggcca ctccaccagc gaagccactt cttccagcag gctttcgctt
     1561 aagtcagcgt taccgccaat cttacgcgct gcttcttcgg catcggcttt aatcttcgct
     1621 ttacgttctt cgtaatcggc gatgactttc ccgcgctcac gcagaatttc cggatactga
     1681 tcggcgttgt cgatggtgaa ttccggttcg cccataaagc ggtggccgcg aatcacgcga
     1741 tcggactgaa tgcccagaat ggttgccgga atgactttgt cgcccagcag cagggtcacg
     1801 gtgtgcaccg gacgcacgaa gtgcacgtcg cttgcacccc agcgcatcag tttcgggatc
     1861 ggcagtttcg ccagcgaagt cgcaaccata ttcggcagca gtgcttcggt gctttcgccc
     1921 ttcacatggg cgcgatacag cagccattcg cctttatcgg tagtcagacg ctcagcctgg
     1981 tcaacggtaa taccacaacc acgcgcccaa ccttctgccg ctttgctcgg ttttccttca
     2041 gcgtcgaacg cctgggcaat cgccgggccg cgtttttcga tttcacgatc tggttgtgct
     2101 tccgccaggt tagctacttt cagcgccaga cgacgcggag cagcaaacca ttgaacggtg
     2161 ccgtgtgcga ggcctgcgtt atccagctcc gcagtaaagt tcgcagcaaa ggactctgcc
     2221 aggctgcgca gtgcttttgg tggcagctct tcagtgccga tttccaccag aaaagttttc
     2281 tcagacatag ccgcctctta cttatctttg ttgcacatcg ggaagccgag ggcttcacgg
     2341 gaagcgtaat atgcttctgc cactgctttg gtcagggtgc gaatgcgcag aatgtagcgc
     2401 tgacgctcgg tgacggagat ggctttacgc gcatccagca ggttgaagct gtgggcggct
     2461 ttcagaatac gctcgtaggc tggcagcggc agcggatttt ccagcgccag cagctgctgc
     2521 gcttctttct cgtactgctc aaagcaggtg aacaggaagt ccacatccgc gtattcgaag
     2581 ttgtaagtgg actgctccac ttcgttctga tggaacacgt cgccgtaggt ggttttaccc
     2641 agcgggccgt cgctccagac caggtcgtaa acgctgtcta cgccctgaat gtacatggcc
     2701 agacgttcca gaccgtaggt gatctcgccg gtaaccggtt tacactccag accaccaacc
     2761 tgctggaagt aagtgaactg cgtcacttcc atgccgttta gccacacttc ccagcccagt
     2821 ccccaggccc ccagagtcgg gttttcccag ttatcttcca cgaaacggat gtcgtgaata
     2881 gtcgggtcca tgcccagctc tttcagagaa ccgaggtaca gctcctgaat attgtccggc
     2941 gatggcttaa tgaccacctg gaactgatag tagtgctgta aacggttggg gttttcgccg
     3001 tagcgaccat cggtcgggcg acgagaaggc tgaacataag cagccgccat cggttctggc
     3061 cccagcgcgc gcagacaggt cattgggtga gaggttcccg cgccgacttc catgtccaat
     3121 ggttgaacaa tggtgcagcc ctggcgagcc cagtaatcct gtaaggtcag gatcaagccc
     3181 tggaaggtcc tggtatcaaa cttttgcata ttatttcgtg ctggatacgt gtggatttaa
     3241 aggaagggat cagtataccc gctggatgga agatatacag tacgaaacgg gaaaaagcag
     3301 ggcttaacgc atggaaagat gcaaaaaatg gccatctgcg tcaaaagaac agacaaactg
     3361 ctctttacgg aaggtataac cgcgcatttc atagcttccc tggaactgtt cgaaggcggt
     3421 gacgtcgatt ttttgctcgc cggtgttata acgttttgcc gcctgatcct tgcataacgc
     3481 ttccatattc aacgaacgca gaggatcaac ctttacccgt tgtgctttct gcacgggtga
     3541 cggtgtagaa caacccacta gcaccataag cgccattgcc ggaaagaaag cgttcatcat
     3601 cattaaatta ccgtctggtt tgcaggtgag tcttattatt tatatcggta tagtggctga
     3661 tatcttacga atttccttaa gcctctatag gctgtagtga taaaactcag attattccag
     3721 tgcgccaacc aaatgattta tcggtcagac tttttttaac cagatacaga gacacagatg
     3781 cagcccaaaa tttactggat tgataacctg cgagggatag cgtgtttaat ggtggtgatg
     3841 attcacacca ctacctggta tgtgaccaat gctcatagtg ttagccccgt cacatgggat
     3901 atcgccaatg ttctgaattc tgcctctcgt gtcagcgtgc cgctattttt catgatttcc
     3961 ggctatctct tttttggcga acgcagcgcc cagccgcgcc atttcttgcg tatcggctta
     4021 tgtctgattt tttatagcgc aatcgcactg ctctacattg cgctgtttac ctccatcaat
     4081 atggagttag cgctgaaaaa cctgctgcaa aagccagtgt tttaccactt gtggtttttc
     4141 ttcgcgattg cggtgattta tctggtttca ccgctgattc aggtgaagaa cgtcggcgga
     4201 aaaatgttgc tggtgctaat ggtggtgatt ggcattatcg ctaacccaaa cacagtgccg
     4261 cagaaaattg acggttttga atggctgcca attaacttat atatcaatgg cgatactttt
     4321 tactacattc tgtatggcat gttgggccgc gctataggga tgatggacac acagcataaa
     4381 gcactgtcgt gggtgagcgc cgcgctgttt gcgactgggg tttttattat ctcacgcggg
     4441 acattatatg aattgcagtg gcgcggaaat tttgccgata cctggtatct ttactgtggg
     4501 ccgatggttt ttatctgcgc aatcgcgcta ttgactctgg ttaaaaacac gctggatacg
     4561 cgtaccattc gcggacttgg cttaatctcc cgccattcat tgggtatata cggattccac
     4621 gccttgatta tccatgcgct gcgcacccgg ggaattgagc ttaaaaattg gccaatactg
     4681 gatattattt ggatcttttg cgcgacgttg gcagcgagtt tgttactttc tatgctggta
     4741 caacgaatcg acagaaacag attagtgagt taagtaaaag cccgttcaca ttggactgac
     4801 cgggcttacg tgaattattc gctgtattct tcctgggtaa cctgtgtttc ttttggcggg
     4861 tttattccgg catcacgaat attcttctgc accgctactg caccaaacaa gcttaagaag
     4921 aaagccagtc cataaaaacc tttttcactg agtaataatg tcgcattcca cagacctacc
     4981 atcagtagtg caacagagat aataaagaca gtcaggcagg tcatataata aatggaagtg
     5041 gtcggtatgc cttcatactt gtcccgaacg gtcttttgat aagacgcagc agaaaacagt
     5101 cctaacacca gtacggcaaa ataataccct ttttcattta actgcatctc tgcattccat
     5161 agccctaaca ggtaggtaac gataccacca acgagagcta tccatgacac aatactaaag
     5221 gccggtgaat aggttgatat tttgttgtcc atcacagtat tcctttcatt cctgaatgtg
     5281 taagagcttt atgttgctca taccgagcga aacaaacgca tttgaccaaa caagcagaca
     5341 aaaaaggcga tggggtagat caccatttca taagcattga taggtgcatt taaaacaccg
     5401 ataaacaaga gaccgatagt gattaacgca actaactggc agatatgggt aaaaaaatta
     5461 tccagttgcc cgagtttttc tgcgcgaaga tatgcatagt taccaaaagt tgctgtcatt
     5521 aacacgccaa gaaaatagcc ttttccactt agcaatgggc atgagatcca tagcccaacc
     5581 agataaacca gcgccccgac aacaaataat agttttgcca cagtttttga ggttttcata
     5641 acgatctcca tatctaccag cgatacatta cgagtaacca acgaaagaca aaactgaaaa
     5701 atgccattaa caaatgattt tcagaataaa ttcatactaa atattaatta attagtgaga
     5761 tat
//
LOCUS       DBKEKI010000208         5750 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.210, whole genome shotgun sequence.
ACCESSION   DBKEKI010000208 DBKEKI010000000
VERSION     DBKEKI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.210"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..207)
                     /locus_tag="ACUQPX_004048"
     CDS             complement(<1..207)
                     /locus_tag="ACUQPX_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HHV9058529.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQS"
     gene            727..1422
                     /gene="minC"
                     /locus_tag="ACUQPX_004049"
     CDS             727..1422
                     /gene="minC"
                     /locus_tag="ACUQPX_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HHV9058530.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1446..2258
                     /gene="minD"
                     /locus_tag="ACUQPX_004050"
     CDS             1446..2258
                     /gene="minD"
                     /locus_tag="ACUQPX_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HHV9058531.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2262..2528
                     /gene="minE"
                     /locus_tag="ACUQPX_004051"
     CDS             2262..2528
                     /gene="minE"
                     /locus_tag="ACUQPX_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HHV9058532.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDTEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2900..3244)
                     /locus_tag="ACUQPX_004052"
                     /pseudo
     CDS             complement(2900..3244)
                     /locus_tag="ACUQPX_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3279..3398)
                     /locus_tag="ACUQPX_004053"
     CDS             complement(3279..3398)
                     /locus_tag="ACUQPX_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HHV9058533.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3359..3544)
                     /gene="ymgJ"
                     /locus_tag="ACUQPX_004054"
     CDS             complement(3359..3544)
                     /gene="ymgJ"
                     /locus_tag="ACUQPX_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HHV9058534.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3645..3818
                     /gene="ymgI"
                     /locus_tag="ACUQPX_004055"
     CDS             3645..3818
                     /gene="ymgI"
                     /locus_tag="ACUQPX_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HHV9058535.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3820..4164
                     /locus_tag="ACUQPX_004056"
     CDS             3820..4164
                     /locus_tag="ACUQPX_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HHV9058536.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4168..4503
                     /locus_tag="ACUQPX_004057"
     CDS             4168..4503
                     /locus_tag="ACUQPX_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HHV9058537.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4560..>5750)
                     /locus_tag="ACUQPX_004058"
     CDS             complement(4560..>5750)
                     /locus_tag="ACUQPX_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HHV9058538.1"
                     /translation="GIEIISVEGESNGEFIKDSRIFAGAYEYSLTRGNTEATNKNWYL
                     TNFQATSGGETNSGGSSAPTVAPTPVLRPEAGSYVANLAAANTLFVMRLNDRAGETRY
                     IDPVTEQERSSRLWLRQIGGHNAWRDSNGQLRTTSHRYVSQLGGDLLTGGFTDSDSWR
                     LGVMAGYARDYNLTHSSVSDYRSKGSVRGYSAGLYATWFADDISKKGAYIDSWAQYSW
                     FKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSFGLEAAKYTWIFQPQAQAIWMGV
                     DHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEKNSGPHGDDFEPFVEMNWIHNSK
                     DFAVSMNGVKVEQDGVSNLGEIKLGVNGNLNPAASVWGNVGVQLGDNGYNDTAVMVGL
                     KYKF"
BASE COUNT         1568 a         1367 c         1332 g         1483 t
ORIGIN      
        1 tgattgtaag ttttcagcgg ctttttcgcc gagatatttc cttgttaact ctggggaaat
       61 gccatctgag ttcacacaaa tatacgggtc gcaaacaacg ccgcacgccg gagattgaag
      121 tccgccagcg tcatgtccag acgccattga tgagaacatc atccccacac ctgacaatcc
      181 gatataaata atacgcatca tattcatagc acatcctgat aatccaaccg tacaacactt
      241 atgctcattt cgtcatcaat ccgtaatcaa aaccgctgac ctggacgagt tacgatgacc
      301 tgacagaagt atcagacatc ttctgcggtt tctatgatag agcaattcta tggcggatat
      361 taagataacg ggtatctgtt ctttaatgat gctgcaaaaa atggaaagtt aagaaagcct
      421 ggcgtggtga aaaaatacac ttgcctcaat actggatatt taaacaggtc tggagatgct
      481 gtcacgatta gggtgcggaa aaaggaagcg taaataagaa gaatcaaaag tacgacggca
      541 atgggttgat tgacaagggt attttttaag ctatgaatca gcgccattta tcacagaata
      601 gacttttact ctgaataaat gggagggtga cttgcctcaa tataatccag actataacat
      661 gccttatagt cttcggaaca tcatcgcgcg ctggcgatga ttaatagcta attgagtaag
      721 gccaggatgt caaacacgcc aatcgagctt aaaggcagta gcttcacttt atctgtggtt
      781 catctgcatg aggcagaacc taaggttatc catcaggcgc tggaagacaa aatcgctcag
      841 gcccccacat ttttaaaaca tgcccccgtt gtactcaacg tcagtgcact ggaagacccg
      901 gtaaactggt cagcgatgca taaggcggtt tcggcaaccg gtttgcgggt tattggcgta
      961 agtggctgca aagatgcgca acttaaagcc gaaattgaaa aaatggggct gcctatcctg
     1021 acggaaggaa aagaaaaagc gccacgtcca gctcccgcac cgcaggctcc agcgcaaaat
     1081 acaatgccgg tcacaaaaac gcgtttaata gataccccgg tgcgttccgg tcagcgtatt
     1141 tatgctccac aatgtgatct gattgttaca agccacgtta gcgctggggc cgaattgatt
     1201 gccgatggga acattcatgt ctatggcatg atgcgcggtc gtgcgctggc aggggcaagt
     1261 ggtgaccggg aaacgcaaat attttgtacg aacctgatgg cggaattggt gtccatcgca
     1321 ggtgaatact ggctgagtga tcaaatccca gcagaatttt atggcaaagc ggcgcgactg
     1381 cagttagtcg aaaacgcttt gaccgttcaa ccgttaaatt gatccctttt tgacaaggaa
     1441 tttctatggc acgcattatt gttgttactt cgggcaaagg gggtgttggt aagacaacct
     1501 ccagcgcggc catcgccact ggtttggccc agaagggaaa gaaaactgtc gtgatagatt
     1561 ttgatatcgg cctgcgtaat ctcgacctga ttatgggttg cgaacgccgg gtcgtttacg
     1621 atttcgtcaa cgtcattcag ggcgatgcaa cgctaaatca ggcgttaatt aaagataagc
     1681 gtactgaaaa tctctatatt cttccggcat cgcaaacacg cgataaagat gccctcaccc
     1741 gtgaaggggt cgccaaagtt cttgatgatc tgaaagcgat ggattttgaa tttatcgttt
     1801 gtgactcccc ggcagggatt gaaaccggtg cgttaatggc actctatttt gcagacgaag
     1861 ccattattac caccaacccg gaagtctcct cagtacgcga ctctgaccgt attttaggca
     1921 ttctggcgtc aaaatcacgc cgcgcagaaa atggcgaaga gcctattaaa gagcacctgc
     1981 tgttaacgcg ctataaccca ggccgcgtaa gcagaggtga catgctgagc atggaagatg
     2041 tgctggagat cctgcgcatc aaactcgtcg gcgtgatccc agaggatcaa tcagtattgc
     2101 gcgcctctaa ccagggtgaa ccggtcattc tcgacattaa cgccgatgcg ggtaaagcct
     2161 acgcagatac cgtagaacgt ctgttgggag aagaacgtcc tttccgcttc attgaagaag
     2221 agaagaaagg cttcctcaaa cgcttgttcg gaggataagt tatggcatta ctcgatttct
     2281 ttctctcgcg gaagaagaac acagccaaca ttgcaaaaga acggctgcag attattgttg
     2341 ctgaacgccg tcgcagcgat acagaaccgc attatctgcc gcagttgcgt aaagatattc
     2401 ttgaggtcat ttgtaaatac gtacaaattg atcctgagat ggtaaccgta cagcttgagc
     2461 aaaaagatgg cgatatttct attcttgagc tgaacgtgac cttaccggaa gcagaagagc
     2521 tgaaataagc ccgctgtaaa aacgcattta tcttcaaggc agaatcattt ctgccttgag
     2581 tttttcatcc ctctcatcca cgttgtggta aagcggcgag tattcttgct gatactcctc
     2641 attgctattt ccacctcccc ctttacacct taaggctgtt cccacgcaca gaacaaaatg
     2701 tttatgcctg gttgagtaaa taaccatgtt gttagtgtgg atatactggg agatgataac
     2761 cccgcaattt cattattcat tgttctccca taggatgagg tttcccgccg taactggcgg
     2821 gctttttttg ccccagaatt ttctctttca acatcctgta aacgaaaact gcgccgaagc
     2881 gcagtttaat cgttgatggt cagaacgacc agttcacacc agccaccgcg ttccacgggg
     2941 actccacacc ggcaccatgg ctatacccca ccccgaaatg cccgcttaac gtactgctga
     3001 atgaggcttt aatacctgcc tggtatattc cacgtctgcc cgacaaatca ttgacaaaat
     3061 taccgtcact attcactttc acccgattat catcgacaaa ttctttgcgc acagccgcct
     3121 tcaggcaggg ttcaatttcc ataccgttcc ccagacgcat gttgtaactc agcgttgcac
     3181 ccagttcacg atatatactg cgggtatcga ctgatttcga ttccatgcca ttggataaat
     3241 gatagtagcg tctaagtgac ctaagctctg ctgcaacatc agcccgcagg caccagacca
     3301 ggggattcat cctgaagaga cagcgcaggt gtattgtgtt caccgctcac caaagacatc
     3361 atgatgaaat gatgatatcc cgcataagaa agaggcattt tttacacgca gtgcgctgaa
     3421 gtgtagttgg ataaaaaatc actcttttca gataatactg tcgtacgctt atctttagac
     3481 aatgaggccg tcggcaaaat aaaatgacta atgtaatcgt tgctgatgaa tatcttctgc
     3541 tcacataaaa atcacacaat aatattgata aatcagatta tcttaccttt acaacattgc
     3601 gttaacagtc aatgacactt cttgaataaa aatattgtca atcaatgagt tattcctcat
     3661 ttaagattat tctcattcac gtaaaaaaca taattcctat catcacagcc acgctcattt
     3721 tgaattatct gaataatagc gagcgttctt tagttaagca gatcttaatt gaagacgaaa
     3781 ttattgtgtg tgcaacttac ttaataccgg acatctaagt tgaagaaaaa aatacttgcc
     3841 ttcgggctta tctcagcatt gttctgctcc acgccagcga tggcggatat gaaccgcacc
     3901 acgaaaggtg cattgctagg cgcaggcgtt ggtttactta ctggcaatgg tgttaatggc
     3961 gtactgaaag gtgcagctgt tggcgctggt gttggtgcag taacagaaaa aggccgcgac
     4021 ggtaaaaatg cgcgtaaagg tgcaaaagtg ggtgccgccg ttggtgctgt cactggcgtt
     4081 ctgaccggca atggcctcga aggggcgatt aaaggtgccg tgattggcgg tacgggtggt
     4141 gcaattctgg gtaaaatgaa ataaggaatg atgatgaaaa aatttgcttt actggcgggc
     4201 ctgtttgttt tcgcaccaat gacctgggcg caggactaca atattaaaaa cggtttgccg
     4261 tcagaaacct atattacttg cgcagaagcc aacgaaatgg caaaaactga cagcgcacag
     4321 gttgcagaaa ttgttgcggt aatgggtaat gccagcgttg ccagccgtga tttaaaaatt
     4381 gagcaatctc cggaactgag cgccaaagtg gtagaaaaac tgaatcaggt ttgcgctaaa
     4441 gacccgcaaa tgctgttgat cacggcaatt gatgacacca tgcgtgctat cggcaaaaaa
     4501 taatatttca tatgtaccgc gtcgatgaat acgacgcggt tttttagcta acggcgggat
     4561 cagaacttat atttcaggcc caccatcact gcggtgtcat tgtagccatt atcacccagc
     4621 tgcacgccca cattccccca gacgctggcc gctggattca ggttgccatt tacgccaagt
     4681 ttaatttccc ccaaattact caccccatct tgttcgactt tcacaccgtt cattgagaca
     4741 gcaaaatctt tactgttatg gatccagttc atttcaacaa aaggttcaaa gtcgtcaccg
     4801 tgcggaccgc tgtttttctc ctgagtacga ataaaggtgc ggaagccgag tcgggtctgg
     4861 atgttgttat ttgcgtcatt ctcaatacgt gagccattgg cttccgtgtg cgcattatga
     4921 tcgacgccca tccagatagc ctgtgcctgt ggctggaaga tccacgtata tttcgccgct
     4981 tccagaccaa aggatttatt cagggcaaaa ccgtaacccg cttccagcga gacggttgca
     5041 cctttcgcgc tataggattc ataggctaat tcatcccctt tcacggagtt tttaaaccag
     5101 ctatattgcg cccaggagtc aatgtatgcg ccttttttac tgatgtcatc ggcaaaccag
     5161 gtggcataca gccctgcgct atagcctctg acactccctt tcgaacgata atccgacacg
     5221 ctggaatgag ttaagttgta gtcgcgggca taaccagcca tcactcccaa acgccaactg
     5281 tcactatcgg taaaaccacc ggttaacaga tcgcccccta actgcgagac gtagcgatgc
     5341 gaggtcgttc tcaactgtcc gttgctgtca cgccaggcat tatgcccgcc aatttgacgt
     5401 agccaaagtc ggcttgaacg ctcctgttca gttacaggat cgatgtagcg cgtttcaccc
     5461 gcacggtcgt tcagacgcat aacaaaaaga gtgttagcgg ctgccaggtt ggcgacgtaa
     5521 cttccagctt cggggcgcag gacgggggta ggcgcaacag taggcgctga acttcctccg
     5581 gagtttgttt caccgccgct cgttgcctgg aagttagtca gataccagtt tttattggtc
     5641 gcttcggtat tacctcgggt taatgaatat tcgtaggcac cggcgaaaat cctcgaatcc
     5701 ttaataaatt ccccatttga ttccccctca acgctgataa tctctatccc
//
LOCUS       DBKEKI010000209         5701 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.211, whole genome shotgun sequence.
ACCESSION   DBKEKI010000209 DBKEKI010000000
VERSION     DBKEKI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.211"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1536)
                     /locus_tag="ACUQPX_004059"
     CDS             complement(<1..1536)
                     /locus_tag="ACUQPX_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HHV9058539.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPALGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPMQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHFSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQLYMLDVGQ
                     GLAM"
     gene            complement(1743..2027)
                     /gene="ihfB"
                     /locus_tag="ACUQPX_004060"
     CDS             complement(1743..2027)
                     /gene="ihfB"
                     /locus_tag="ACUQPX_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HHV9058540.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2187..3860)
                     /gene="rpsA"
                     /locus_tag="ACUQPX_004061"
     CDS             complement(2187..3860)
                     /gene="rpsA"
                     /locus_tag="ACUQPX_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HHV9058541.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3971..4654)
                     /gene="cmk"
                     /locus_tag="ACUQPX_004062"
     CDS             complement(3971..4654)
                     /gene="cmk"
                     /locus_tag="ACUQPX_004062"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HHV9058542.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4827..5591)
                     /gene="ycaL"
                     /locus_tag="ACUQPX_004063"
     CDS             complement(4827..5591)
                     /gene="ycaL"
                     /locus_tag="ACUQPX_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HHV9058543.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1445 a         1505 c         1343 g         1408 t
ORIGIN      
        1 cattgccagc ccttgcccga catcaagcat gtacagctgc cactcgtcag gtcgaggttt
       61 ttgccacagc ggccagcaca tcagcaagcc tccagccaca cacattgctg gcaacgttcg
      121 ccaggcgttt aatcgccata ccaccagtaa gaaccatggg gaaaatgata gccattgcca
      181 acattcagca atgttgatcc acccttccgg caaactcttt aacccccaga aaagtaaagc
      241 caaagaccgg tcggcaagaa accataaccc ctgttccagg attaacggcc cgctaaaatg
      301 cacaaccatc gcggcgagga tcaacggaac cgtgataaat gtcaccaagg gaattgcaaa
      361 tagatttgca ataaacgagg tcagactaat gccatgaaat atgacgattt gcatgggcat
      421 aagcaggagt gtgattccca gttgcagatg gatgagggaa acaactgcac gcaataccgg
      481 cggcagttgc cactcaggac agggaaacca ctgataccaa aaaatcagtg ccgcgaccgc
      541 agcggcagag agccataaac tttgcgagag aatggcaaca ggatccatca gcaaaattgc
      601 cgccagacaa catatccata catcccagcc actccactgt cgcccactta acttaagcat
      661 tccccacgta gcaagcgcca ccacggtacg caatgcagga ggttgcattc ccgtcaacca
      721 ggcataaaaa gcagcacagc agattccgcc aattaatggt atttgccagt ggatccagcg
      781 cccaggcaga aaaatttgtc cactgcgaat gagtccggca gccagcaacg ccgcaaaagc
      841 gatgtgcaat cccgatatcg ccattaaatg cgccgttcca gtatcgcgca tgatattttt
      901 gatttctttg gggacggata accgttcccc catacctaaa ccaagaataa ccgcattcca
      961 cggatagggt tgcagcgttg tttgtagtga cgccagatac tgtgcacgca ggctacaatt
     1021 cggttcaatg acacttgcct gcagaaaacg gccggtgagc ggctgatgct gggcaatggc
     1081 ataacgctgg ctatcaaagc cgccatcatt aagttggcca tgaactgcac gaactttgag
     1141 tttcattgac cattgttgtc cagcacaaac cgcttgcgga agatattcgc catacagcac
     1201 aaggcctagc gcagggaata tacgtcgacc ttgtagataa gtaatttgac cgtaatgagt
     1261 ggtcatgccg tcagttgcag tgatctcaac aattgcatcc tgcgtcgcgc cagttaaggt
     1321 ttctcctgcc cacaaaattt gctttgctga taatatgccc caaacgaaaa acagcaacgt
     1381 cagcgcgata taacggacgg ttttaacagg gataaatgcc agtacgcagg cgaagagagt
     1441 cagaaacgca agggttaatg tcccaggcaa ttggggcaaa atcagcaacg gaaaaattcc
     1501 gcaaattatg catacgccga ctgtcgttat tttcatgcac ccctccctgt ttatggaaag
     1561 tgtgcaactt tgttgggcgg ttgtctggtg ggaattttgc gttccccgag gcacattcag
     1621 aagaatttaa cgaaagggtg aaaactgctc aaaactctgc gaggcagctt ccagttcctg
     1681 attagagaat tgaaaaaagc acccgacagg tgcttttctc gcgttcaagt ttgagtaaaa
     1741 acttaaccgt aaatattggc gcgatcgcgc agttctttac caggtttaaa gtgaggaacg
     1801 tattttcctt ccagttctac tttatcgcca gtcttcggat tacgtccggt acgtggtgcg
     1861 cggtagtgca aagagaaact gccgaaaccg cggatttcaa tacgctcgcc ctgcgcaaga
     1921 gtcgaggcca tatgctccag catctctttt actgcatctt caaccgtctt ggcgggaatg
     1981 tgcgattgct gggtggcaag tctttctatc aattctgact tggtcatgat tcctccggtt
     2041 ccttaaaggc aaattaagcg gctgctgttg ctgcattgat taagggcggc cgtagccgcc
     2101 cttagtgctt gattacagga cgaaacctgc aatctgtcaa gtaaactcaa caaacttcgg
     2161 aataaaaatc ccgaagagtc agagaattac tcgcctttag ctgctttgaa agcttcagcc
     2221 attgcgttgt tggagaagtt tgcatcttcc tgtttgttaa cagttgcgat tgcatctttc
     2281 tcgtcagctt cgtctttcgc acgaacagac aggctgattg cgcggttttt acgatcaacg
     2341 ccggtgaatt tagcttcaac ttcgtcgcca acgctcagaa ccagggtagc gtcttcaacg
     2401 cggtcacggg atgcttcaga agcacgcagg taaccttcaa cgccgtcagc cagttctacg
     2461 gttgcgcctt tagcgtcaac tgcagttact ttaccggtta cgatagcgcc tttcttgttc
     2521 agagcaaccc agttgttgaa cggatcttct gcgagctgtt taacgcccag ggagatacgt
     2581 tcacgttctg cgtcaacctg cagaacaact gcagcgattt cgtcgccttt tttgtattca
     2641 cgaactgctt cttcgcctgc aacgttccag gagatgtcag acaggtgaac caggccgtcg
     2701 atgccgccgt ccaggccgat gaagataccg aagtcagtga tagacttgat tttaccttca
     2761 acacggtcgc ccttgttgtg ggtttccgcg aactgctgcc acgggttagc tttgcactgt
     2821 ttcagaccca gggagatacg acgacgttct tcgtcgatat ccagaaccat aacttccact
     2881 acatcgccaa cgttaacaac tttggacggg tggatgtttt tgttggtcca atccatttcg
     2941 gaaacgtgta ccagaccttc aacgccttct tcgatttcaa cgaagcagcc gtagtcagtc
     3001 aggttggtca cgcgaccagt cagtttggta ccttccggat aacgtttagc gatagctacc
     3061 cacggatctt cgcccagctg tttcaggccc agggatacac gggtacgttc gcggtcgaac
     3121 ttcagcactt taacagtgat ttcgtcgccc acgttgacga tttcgctcgg atgcttaacg
     3181 cgtttccagg ccatgtcagt gatgtgcagc aggccgtcaa cgccgcccag atcaacgaat
     3241 gcaccgtagt cagtgaggtt cttaacgata cctttaactt ccatgccttc ctgcaggttt
     3301 tccagcagct gatcgcgctc tgcgctgttt tcggattcga taacggcacg acgagaaaca
     3361 acaacgttgt tgcgcttctg atccagcttg attactttaa attcaagctc tttgccttcc
     3421 aggtgcagag tgtcacgcac cggacgaacg tctaccagag aacctggcag gaacgcacga
     3481 ataccgttca gctcaacagt gaagccgccc ttaactttgc cgttgataac accggtaaca
     3541 gtttcagcat cttcgtaagc tttttccagc gtgatccagg cttcgtgacg tttagctttc
     3601 tcacgggaca gcagagtttc accgaagccg tcttctactg cgtccagagc aacgtcaact
     3661 tcgtcaccta cctggatttc cagctcgccc tgggcgtttt tgaactgctc agccgggatg
     3721 gcggactcag atttcagacc agcgtcaacc agtactacgt ctttgtcgat agcaacaaca
     3781 acgccacgaa cgatagaacc cgggcgggtt tcgatttctt ttaaggactc ttcaaagagt
     3841 tgagcaaaag attcagtcat gtttaatctt caggtttata tttaacgtcc acctggctcc
     3901 atgccggatg gggttgttta acatacccgc tgtcattcca ttgcaacggg ggtactgcaa
     3961 attcggtcgc ttatgcgaga gccaatttct ggcgcgcgta ttgtagcgct ttttcaatca
     4021 cttgctcaat gcttaaggtg gtggaatcca acactaaagc atcggctgcc ggaaccagtg
     4081 gcgctaccgc tcggttacga tcgcggtcgt cgcgttcttt gatctcggcc aaaaggcgct
     4141 cgaagttaac actaaagccc ttctcctgca actgtagcat gcggcgatgc gcacgttctt
     4201 ccgaggaggc gtcaaggaaa attttcactg gtgcatcagg gaataccacc gttcccatgt
     4261 cgcggccatc ggcaatcaga cctggtaatt cgcgaaacgc gcgttggcga cgcaataatg
     4321 cttcacgaac gcgtgggaat gccgcgactt gtgaagccgc attcgccact tcctgagtac
     4381 gaatttcgcc gctgacatct tccccttcga ggatcacttc cagattgcca ttggtcgaca
     4441 caaaacgtac atccagatgg gatgccagcg gtaccagcgc atcttccgac gcaacatcaa
     4501 catggtgatg taatgccgcc aatgccagta cgcgataaat tgcacccgag tccagcagat
     4561 gccattgcaa cgcttccgcc atagccttac acaaggtgcc tttccctgca ccgcttgggc
     4621 catcaatggt aataaccggg gcaattgccg tcatctttat ctccttaaaa caggcgtacc
     4681 gttaacataa ccgcgcgcat tatacgcgcc gacgccacaa atcgttaacg ttgtgtgcaa
     4741 aaaccagttt tgtctgtatt cagatgggga tttattgcgc aattatagcg ggctggcaga
     4801 agaccagccc gaaagatgac aatgatttac ttaccagagg cgatacgatc acggatgtgt
     4861 tgcgcacgct ctgttgatgg tgggtgagag tcaaacatgg actgggtgcg accgccatcc
     4921 aggctagcca gtttttcaaa gctgccaacc agcccctggg tgctgatgcc acgtttcttc
     4981 aacagatcaa aagagaaatc atctgcttcg gactctttat cacgggagta tttagcatta
     5041 atagcgcctt ctgcgatatc acccaattgt gagcgggaaa gctgggaagc cacaccgctg
     5101 gtagctgaaa tggcatcgcg tgcggcaacg atcgcataag aagctttcat ttcagccagc
     5161 gagtgaccca acgcgacgtg gcccagttca tggcccagaa caccttcaat ttcgttgtca
     5221 ttcatcatgt ccatcaggcc actgtagaca cgaacacagc cgttcgccat cgcccatgcg
     5281 ttgacgtcgc tggtcatata aaccttatag ttgaccggcg tgccattaat attgttaccc
     5341 aatgctttgg cgatttttgc cagacgttta ccgtacttgc tggatttact tgcgacttga
     5401 ttgccgctgt ccatttcggc acagccctga ttggcaatcg cttttgcatc ggcatcgctt
     5461 aatgttgctg ctttataggc atttaaaccg gagctgatag ccatattggt atcaataccg
     5521 tgggtatttt gacaccctgt cagtaatgct gcagaggtcg caatcgccag cagtaattta
     5581 gtattcttca tgtgaatcat ccgttgatta atcatatata aatattatgt atcaacaact
     5641 tacattgaca agcgacgcta ccattaatca aaatgactca caaggtccga aaaaaaacgc
     5701 c
//
LOCUS       DBKEKI010000210         5694 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.212, whole genome shotgun sequence.
ACCESSION   DBKEKI010000210 DBKEKI010000000
VERSION     DBKEKI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.212"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..307)
                     /locus_tag="ACUQPX_004064"
                     /pseudo
     CDS             complement(1..307)
                     /locus_tag="ACUQPX_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(451..3759)
                     /gene="icsA"
                     /locus_tag="ACUQPX_004065"
                     /gene_synonym="virG"
     CDS             complement(451..3759)
                     /gene="icsA"
                     /locus_tag="ACUQPX_004065"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HHV9058544.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNDEKLLTPVDGLSPLGAGEDGMDAWYITSSNHSHAS
                     RTKLRINTDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSMINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKDRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4288..5490
                     /gene="virA"
                     /locus_tag="ACUQPX_004066"
     CDS             4288..5490
                     /gene="virA"
                     /locus_tag="ACUQPX_004066"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HHV9058545.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1773 a         1288 c          936 g         1697 t
ORIGIN      
        1 tttataccta ctctgaccaa gtctggataa gaaaggcaca cctgcacatt tctacttgga
       61 aatgttttct tttaccattt tgctcaaaaa tttcaacatc caatattcct cgtacagaac
      121 tactcaagtc ctgaatagaa aaagttccag ctggtacagt agagtcatat attactcgac
      181 cttgttgaga tacaacaaca cgtgcattag tttctgcaat accagtaatc tgaggcgcat
      241 aaccacgaat atttggcggt agcattcgtt catcgctttc taatgcaatt cctgtgtaac
      301 gccaagcaac agcgcattct ggcgcatgat ctggtaaaca cgtttggcat tgatcgcagg
      361 cataccatca tgtgcacaaa aacccaccga gcttttacac ggtgggtccc agagaaatgc
      421 aggacatcaa cacgccctgc atttttatta tcagaaggta tatttcacac ccaaaatacc
      481 ttgggtgtct ctgtaactgt tattacccaa ttgctgagat acgtttcccc agatgcttag
      541 gttatctgca agacgccccc ccacaccagt tttgagttca atcacattac ggttgcctat
      601 ctggtgattt gacatgccat tcataattac accatattgc tttgagctcc atttccaatt
      661 cacttccaaa aatggttcga acttacgaag agtctcctta tcgatacaac tctgactaat
      721 taaggatgta cgcaatccca attttgcttg aatattattg accccagagg atgtgaccat
      781 agtgccattg tgttcaatat agtcgtttgc ttttacccca tgcctagtta tctggacttg
      841 tggattgaga tacaatgcgt tgtcaatatt attatgagca gtccagcgta tggttgggta
      901 gatatagcca gcttccaaag ctcctgttat tcctttgctg gaatatttct caccagttaa
      961 cccatctcct ttgactgtat tattaaacca accgtactgc aaccaagcat ccatatatag
     1021 acctgttcgt tctttttcat cctgaaacca agaactgtac aaaccggcag tataaccgct
     1081 cacagttcct cgtgaactat aactagtata cttattatga gttttgctat cctgatatcc
     1141 ataagctccc ataataccca catgcaagcc atcatgatca gtgaagtttg tcttcaaaat
     1201 atctccacca atctgataaa ccatcctgtt agctgtggtt tttaattgtc tatcagctaa
     1261 attatgctca ttatgtccac caacagtacg aatccacatg gtggtttcgc tgagctgacc
     1321 tgtagtagga tcaacaatag tgctaccgtg tcggtcatag agagaaggac taaacatcgt
     1381 gttcattgct atcaggttag caatataact gccagcttca gggcgccatg tgtgaatact
     1441 accttcaccc aaattaagtg aagatacaag ctgtgaacta gctcggcgac tactcatttg
     1501 agtagactct tgattgctca tctgttttga ttcttgatta tccatctgac tagttagata
     1561 ccacttattg gtattcagtc cagatacagt gccctgtttc aggcgatagt cataactacc
     1621 agcaacaata cggtctttct gaataaaagc attcttatca gaagaatctg tcgaaataat
     1681 ttgaacacct tcaagagttt tatctccaga gcctccctca tttgtcactg caacataggt
     1741 aataccagac gcatggcctg caatttccag ccggtcagtt ttactgttgt cattgccgag
     1801 aaccgcgccc aaagataata ttccgccctt tccatgccaa ttacctttct gaacataggt
     1861 ctgaccgaca tttgacgaac tattattgag aataacatga ccgtttttct ccagagtcat
     1921 gttacctgtg acaatgacgg ggtcaggcaa aaaaggggca ttaatattat tgattagcac
     1981 agtcccacta tttaataaag tgttaacagt ttggttcatg cctgaaaaat tcaaggtagc
     2041 acctttatta acaataacac cagcggtacg tgtcatagct tcaactgtcc ccatttttag
     2101 aatacctgca ttgatgttgg tgaaaccaga gtaggtattt ttttccgcca aaatgagagt
     2161 tccctcccct gattttgtta gggattttcc gtcccagcta tttatattat gatggctgag
     2221 attatctgcc agaatagtac taacagtaag ttcagcgttt tctttcagat tgaactctcc
     2281 atgactgtcg ccatctgtat cattccagct caaaccgtag atcaatttat tctggtcact
     2341 gatatagctt tttgtatagt caatagcacc agtagtgagt aacggattga tgatagatat
     2401 attagaaaca ctgaaacctg ttatcccttt atctgcactt agcagtttat agtccttctg
     2461 cgcccagtta tttataatag cttccacttt tacattgttg ccaaatgaca tatttccaga
     2521 agcttccaga ctactgattc cagctaaatt taaagaactg ttctcaccaa aagaaacgtc
     2581 acctgaaaca gtgaatttgt tacctgatag tgaaacagtt gtacaatcac cagcacataa
     2641 atcaccttca atagttggtg tggtcccagt gtaatcattg ttgataattt tcagaatgga
     2701 tgaattgtta acctgtacat caccagtaat gacagaacct tcatggagta ttaatgaatt
     2761 gtttccacca gtgatgtgga tagcattacc tgctacattt tgtgttccat catcttcttt
     2821 acctgaaatt atatatccgc tattagcaat ggtcatattg ctaccattta tagcatcccc
     2881 ccaatgtcca ccattaccgc ctaaaatagc tccattgttg atcatagaca ggttatctcc
     2941 tgtgatagca ttaccaccgt agccatcata accataaccg taagcagcac ctccgttgcc
     3001 tccagcgaac gtgcccttat tgattacaga gaggctgctt cctgtgatgg catcacctcc
     3061 attaccgcca tcaccctcac cataattgtt acctccatta ccaccggaaa tagtcccttt
     3121 gttgattatg ggtagattga ctcctgtgac ggcatcacct ccattccccc catcactatc
     3181 actataacta tcgccaccat gtccacctga aataatttct ccatttatta taaacagatt
     3241 gtctcctgta acagcctcac caccatcacc gttatggtca gcaccgctac cgccgctacc
     3301 accaagaatc atgccttgat tgattataga caagtcagat ccggtaatag agtcaccacc
     3361 attaccgcca caactattac catcattatt atcaccagca ccaccatgac ctgcgctaat
     3421 cataatatca gtgttaatcc gtagcttagt tctagatgca tgagagtggt tggaagaagt
     3481 tatataccac gcatccattc catcttcacc agctcccaag ggagaaagtc catcaacagg
     3541 tgttaataat ttttcatcat tgtcctctga aaaatgaagt tcttgcgtac ccgaaagagg
     3601 aatagcaaaa gctattggcc ccccgagcaa cagggatgca ccaacaacaa aaggagaaaa
     3661 agacaatttg catgtttttt cctttaccgt aggtaattct ccggcccccc cctgtgaaca
     3721 ttgggtcata ttacaaaaaa atttgtgaat ttgattcatg cactatatta tcagtaagtg
     3781 gttgataaac ccctaaaaag attccgtttt atccggaata aagggacgat atatgcaaaa
     3841 acatattaaa caaagcctca accaaaacat gagagctgtg ttatatatca atgcaacggg
     3901 agaaatttac ctgtgtcttg ctttgaaaaa aagaggggga aagctatatg ttcgataata
     3961 aattaataat tttactatga tagtctgctt cagaccgcaa cttttcagtt ctttctgtac
     4021 gcttgcccaa agtttaacca ctttgttttt ctatacaccc aatggttcta tctgcccctt
     4081 gatgttaatc cccaaagcca ataacatgaa tgtattcata tcacaacttt cctaccagac
     4141 tctccaatgc aataactgca taattgtttt tagtggaaat gtatcgtttt cttaaagaga
     4201 agaataacat tccactttat tatgttccca atttaattat attaatatat attggcatta
     4261 atagaaaaat acatcaggag aaatcaaatg cagacatcaa acataactaa ccatgaaaga
     4321 aatgactcat catggatgtc aactgtcaaa tcaactacag aagtaagttg gaataaattg
     4381 agtttttgtg acgttttact gaaaataata actttcggca tatattctcc acacgaaact
     4441 ctagccgaaa aatatagcga aaaaaaacta atggatagtt tctcaccatc actatcacaa
     4501 gataaaatgg acggagagtt tgcccacgca aacatagatg gcatttcaat acggctatgc
     4561 cttaacaaag gcatatgttc ggttttttat cttgatggag ataaaataca atccactcaa
     4621 ctttcatcaa aagaatataa taacttgtta agttcactcc cccccaaaca attcaactta
     4681 ggaaaagtac atactattac agctccggta tcagggaatt ttaaaactca caagcctgca
     4741 ccagaagtta ttgaaacagc cattaattgc tgtacctcga taataccaaa cgatgattac
     4801 ttccccgtta aagacactga ttttaatagt gtttggcatg atatttacag agatattcga
     4861 gcatctgaca gcaatagtac aaaaatatat ttcaacaata ttgaaatacc actaaaatta
     4921 attgcagatc taattaatga gttaggaatt aatgaattca ttgatagcaa aaaagagctt
     4981 caaatgctaa gttacaacca agtaaataaa ataatcaatt caaacttccc ccaacaagac
     5041 ctatgttttc aaacagaaaa acttttgttt acttcactct ttcaagaccc tgcatttata
     5101 tctgcactaa catctgcatt ctggcaatct cttcacatca cgtcttcctc tgtggaacac
     5161 atatatgccc aaattatgtc cgaaaacata gaaaacaggc ttaatttcat gcctgaacaa
     5221 cgagttatta acaattgtgg acatattata aaaatcaacg cagttgtccc taaaaacgac
     5281 actgcaatct ctgcctctgg ggggagggct tacgaagtta gctcatcaat attaccatcg
     5341 cacataacat gtaatggtgt tggtattaat aaaatagaaa caagctatct cgttcatgct
     5401 ggaacacttc caagcagtga agggcttcgc aatgctatcc ctccagaaag tcgtcaagta
     5461 tcatttgcca tcatatctcc tgatgtttaa gccaatatta tattggctgc cagactgtaa
     5521 aaaaacgttc ttcagaacag ctctataaac tattaaacca aagctaaaag gatggggacc
     5581 aatagaatct cagcccatcc tttttatatc attatgaacc gccccggaaa tcctggagac
     5641 taaactccct gagaaagagg taaacaggat gactaaaaat acacgttttt cccc
//
LOCUS       DBKEKI010000211         5675 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.213, whole genome shotgun sequence.
ACCESSION   DBKEKI010000211 DBKEKI010000000
VERSION     DBKEKI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.213"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(23..760)
                     /gene="bamD"
                     /locus_tag="ACUQPX_004067"
     CDS             complement(23..760)
                     /gene="bamD"
                     /locus_tag="ACUQPX_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HHV9058546.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            895..1875
                     /gene="rluD"
                     /locus_tag="ACUQPX_004068"
     CDS             895..1875
                     /gene="rluD"
                     /locus_tag="ACUQPX_004068"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HHV9058547.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHQTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1872..2603
                     /gene="yfiH"
                     /locus_tag="ACUQPX_004069"
     CDS             1872..2603
                     /gene="yfiH"
                     /locus_tag="ACUQPX_004069"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HHV9058548.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2733..5306
                     /gene="clpB"
                     /locus_tag="ACUQPX_004070"
     CDS             2733..5306
                     /gene="clpB"
                     /locus_tag="ACUQPX_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HHV9058549.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1411 a         1363 c         1537 g         1364 t
ORIGIN      
        1 cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg attttcgcta
       61 ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc agcggcagcg
      121 catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg cggttaacga
      181 cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca tatttcgcca
      241 gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga ctgttcggat
      301 agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga ggatcgcgat
      361 cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc atattggtca
      421 ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg cgaataaaac
      481 gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg tagatgagat
      541 ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac gcttccagtt
      601 gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc gcgtaaattt
      661 catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc aaaaacaggc
      721 ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga ctttcctcaa
      781 aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac aatagcacac
      841 tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt atatatggca
      901 caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg cttagatcag
      961 gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg gatcctcgac
     1021 cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt attgggtggc
     1081 gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc gcaggatatc
     1141 cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc gcgcgaccta
     1201 gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt gcttcattac
     1261 tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga taaagacacc
     1321 actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt cgaatctttg
     1381 caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat gaccgctggc
     1441 ggtacagtag atgaacccat cagccgccac cagaccaaac gtactcacat ggcggtgcat
     1501 ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg tgtgcacacg
     1561 cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca tatggcccat
     1621 atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc gccaaaaggt
     1681 gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct tcatgcaacc
     1741 atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc gcctattcca
     1801 caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca taaggatgaa
     1861 gtggactggt tatgagtaag ctgattgttc cgcagtggcc gctgccaaaa ggtgttgcgg
     1921 cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca ctcaacctcg
     1981 gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga ctttttgctg
     2041 cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa gatgtgctta
     2101 agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc aatacgcctg
     2161 gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc aatcgagcgg
     2221 gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc gtgctggaag
     2281 agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta gggccggcaa
     2341 ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg gcagtagacg
     2401 ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat atttatcagc
     2461 ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc gaccgttgta
     2521 catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc ggtcgtatgg
     2581 caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg cgtgattttc
     2641 ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca tttaatctcc
     2701 agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt actaataaat
     2761 tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac caattcatcg
     2821 aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt agtcctttat
     2881 taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag gcattaaatc
     2941 gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat ctggtgcgcg
     3001 ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc tcgtcagaac
     3061 tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg aaagcagcag
     3121 gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt gaaagcgtga
     3181 acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc gaccttaccg
     3241 aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa attcgccgta
     3301 ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt gaacccggcg
     3361 tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc gaagtgccgg
     3421 aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg gctggggcga
     3481 aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt gccaaacagg
     3541 aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg ggtaaagccg
     3601 atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt gaattgcact
     3661 gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat gctgcgctgg
     3721 aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc attgcgattc
     3781 tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact gacccggcaa
     3841 ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg ccggataaag
     3901 ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac tcaaaaccag
     3961 aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa caggcgttaa
     4021 tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa gaactgagcg
     4081 acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag gcatcgcttt
     4141 ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt gaacaggctc
     4201 gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc ccggaactgg
     4261 aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg ttgcgtaata
     4321 aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt ccggtttctc
     4381 gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg caccatcgcg
     4441 tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt agccgtgcgg
     4501 ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca acgggtgtgg
     4561 ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac gaggcgatgg
     4621 tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg gttggtgcgc
     4681 ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg cgtcgtcgtc
     4741 cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc ttcaatattc
     4801 tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg gtcgacttcc
     4861 gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag gaacgcttcg
     4921 gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc cataacttcc
     4981 gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg ggtgaacagc
     5041 acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg gaagaacgtg
     5101 gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac ggttacgatc
     5161 cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa aacccgctgg
     5221 cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg gaagttaatg
     5281 aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct cgtttttgtc
     5341 tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaagtg agcgaacgat
     5401 aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc gccaccactg
     5461 acacggaaca acggcaaaca cgccgccggg tcagcggggt tctcctgaga attccggcag
     5521 agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca caccccgcgc
     5581 cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat ctgtgtgggc
     5641 actcgaagat acggattctt aacgtcgcaa gacga
//
LOCUS       DBKEKI010000212         5674 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.214, whole genome shotgun sequence.
ACCESSION   DBKEKI010000212 DBKEKI010000000
VERSION     DBKEKI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.214"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..980
                     /gene="narU"
                     /locus_tag="ACUQPX_004071"
     CDS             <1..980
                     /gene="narU"
                     /locus_tag="ACUQPX_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HHV9058550.1"
                     /translation="CGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQL
                     VAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSR
                     ASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPF
                     IGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGL
                     FLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQREAVTETAAALGFISAIGAV
                     GGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK"
     gene            1062..4802
                     /gene="narZ"
                     /locus_tag="ACUQPX_004072"
     CDS             1062..4802
                     /gene="narZ"
                     /locus_tag="ACUQPX_004072"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HHV9058551.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVCQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EEIGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFQEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            4799..>5674
                     /gene="narH"
                     /locus_tag="ACUQPX_004073"
     CDS             4799..>5674
                     /gene="narH"
                     /locus_tag="ACUQPX_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HHV9058552.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDL"
BASE COUNT         1329 a         1531 c         1548 g         1266 t
ORIGIN      
        1 tatgcggttt tgcaggtgca aactttgctt cgagcatggg caatatcagt ttcttctttc
       61 caaaagccaa acaagggagc gctcttggga ttaatggcgg attaggaaac ttaggtgtaa
      121 gtgtaatgca gctggttgca ccgctggtca tttttgtacc tgtatttgcc tttctcggcg
      181 tcaatggcgt accgcaggcc gacggttcgg tgatgtcgct ggcgaatgcc gcatggattt
      241 gggtaccgct actggcgatt gccacgatcg ccgcctggtc agggatgaat gatatcgcca
      301 gttcacgcgc ctcaattgcc gaccagctcc ctgtcttaca acgcctgcat ctctggctgc
      361 tgagcctgct ttaccttgcc accttcggtt cgtttatcgg tttttctgcg ggttttgcca
      421 tgctggcaaa aacccagttc ccggatgtga atattctgcg cctggcgttc tttggcccat
      481 ttattggtgc catcgcgcgg tcggttggtg gtgctatttc cgataaattc ggcggcgtgc
      541 gggtgacgtt gatcaacttt atttttatgg cgattttcag tgccctgctg ttccttacct
      601 taccgggcac aggctccggt aatttcatcg ccttttacgc cgtatttatg gggctgtttc
      661 tgaccgcggg tctgggaagt ggttctactt tccagatgat cgccgtcatc tttcgccaga
      721 taaccattta tcgggtgaag atgaaaggcg gtagtgatga gcaagctcag agagaagccg
      781 tcaccgaaac ggcggcagcg ctgggcttta tctcagccat tggcgcagtg ggcggctttt
      841 ttattccgca ggcatttggc atgtcgctca atatgaccgg ctctccggtc ggcgcgatga
      901 aagtgttttt aatcttctac atcgtttgtg tgctgctgac ctggctggtt tatggtcggc
      961 ggaagttcag ccaaaaataa gagatcgatg cctgatgtac aaccgtgtcg catccgacaa
     1021 ggcatcagat cccaattcat tcctggagca ggagtcatgt catgagtaaa cttttggatc
     1081 gctttcgcta cttcaaacaa aagggcgaaa cctttgccga tggtcacgga caggtgatgc
     1141 atagcaaccg cgactgggag gacagctatc gccagcgttg gcagttcgac aaaatcgtgc
     1201 gttccaccca cggcgttaac tgtacaggct cctgtagctg gaaaatctac gttaaaaatg
     1261 gtctggtgac ctgggaaatc caacagaccg actacccgcg cactcgccct gacctgccca
     1321 atcatgaacc tcgcggctgc ccgcgtggcg caagttactc ctggtatctt tacagcgcta
     1381 accgcctgaa atacccgctc attcgtaaac gactgattga actgtggcgc gaagcactca
     1441 aacaacacag cgatccggta ctggcgtggg catcgattat gaacgatccg caaaagtgcc
     1501 tgagctacaa acaagtgcgt gggcgcggcg ggttcatccg ctccaactgg caggaactaa
     1561 accagctgat tgccgccgct aacgtctgga ccatcaaaac ctacggcccg gatcgcgttg
     1621 ccggtttctc gccgatcccg gcgatgtcga tggtttctta cgccgccgga acgcgttatc
     1681 tgtcgctgct tggcggcacc tgtttaagtt tctacgactg gtattgcgac ctgccgcccg
     1741 cctcgccgat gacctggggc gagcaaaccg acgtaccgga atctgccgac tggtataact
     1801 ccagctacat catcgcctgg gggtctaacg taccgcagac acgtacgccg gacgcccact
     1861 tctttaccga agtacgctac aaaggcacta aaaccatcgc cattacccct gactactctg
     1921 aagtggccaa attgtgcgac cagtggctgg caccgaaaca aggcactgat agcgccctgg
     1981 cgatggcaat ggggcatgtg attttaaaag agtttcatct cgataatccc agcgactact
     2041 ttatcaacta ctgccgccgc tacagcgaca tgccgatgct ggtaatgctg gagcctcgcg
     2101 acgatggtag ctacgttccc gggcgcatga tccgcgcatc tgacctggtg gatggactgg
     2161 gcgaaagcaa caatccgcag tggaaaaccg tagcagttaa taccgcaggt gaattggtag
     2221 tgccgaacgg ttcgattggt ttccgctggg gagaaaaagg caaatggaat ctggaatcca
     2281 ttgccgccgg tacggaaacc gaattgtcgt taaccctgct cggtcaacat gacgctgttg
     2341 caggcgtggc cttcccctac tttggcggca ttgaaaatcc gcattttcgc agcgtaaaac
     2401 acaatccggt gctggtgcgc caattgcccg ttaaaaacct gacgttagtc gatggcaaca
     2461 cctgtccggt ggtcagcgtt tatgatttgg tactggcgaa ttacggcctc gatcgcgggc
     2521 tggaagatga aaacagcgcg aaagattacg ctgaaatcaa accgtacacc ccagcctggg
     2581 gtgagcaaat taccggcgta ccgcgccagt atattgaaac catcgcccgc gaatttgccg
     2641 ataccgccca taaaacgcat gggcgctcaa tgattatcct cggcgcaggt gttaaccact
     2701 ggtatcacat ggacatgaac taccgcggga tgatcaatat gctgatcttc tgcggttgtg
     2761 tcgggcaaag cggtggcggc tgggcacact atgtctgcca ggaaaaactg cgcccacaaa
     2821 ccggctggtt gccactggcc tttgcgctcg actggaaccg accaccgcgc cagatgaaca
     2881 gcacctcgtt tttctacaat cattccagcc aatggcgcta tgaaaaagtc tctgctcagg
     2941 agttactttc accgctcgcc gatgccagta agtacagcgg tcatctgatt gatttcaacg
     3001 ttcgcgccga acgtatgggc tggctacctt ctgcgccgca gctggggcgt aacccgctcg
     3061 ggattaaagc tgaagccgac aaggccggat tatcccccac agaatttacc gcccaggcgc
     3121 tgaaatcggg cgatttacgt atggcctgcg aacaaccaga tagcggcagc aatcatccgc
     3181 gtaatttgtt tgtctggcgc tctaacctgc ttggctcctc cggcaaaggc cacgagtata
     3241 tgcagaagta tctgctgggg accgaaagcg ggattcaggg cgaggaaatc ggtgccagcg
     3301 acgggatcaa accggaagaa gtcgagtggc aaactgcggc gattgaaggc aagctcgacc
     3361 tgctggtgac gctcgacttc cgcatgtcca gtacctgcct gttctccgat atcgttctgc
     3421 ccaccgccac ctggtatgaa aaagacgata tgaacacctc ggatatgcat ccatttattc
     3481 atccgctttc tgcggcggtc gatccggcct gggaatcacg cagcgactgg gaaatctaca
     3541 aaggtattgc caaagcattt tcgcaagtgt gcgtgggcca tcttggcaaa gaaaccgacg
     3601 tggtattaca accactgctg catgactctc cggcagagct ctcacagccg tgtgaagtgc
     3661 tcgactggcg caaaggcgaa tgcgatctga tcccgggtaa aaccgcgccg aatattgtgg
     3721 cggtggagcg cgactaccct gctacgtatg aacgctttac ctcgctcggg ccattgatgg
     3781 acaaacttgg caatggcggt aaagggattt cgtggaatac ccaggatgaa atcgatttcc
     3841 tcggcaaact caattacacc aagcgtgatg gcccggcaca ggggcgtccg ctgattgaca
     3901 ccgccattga cgcttcagaa gtgattctgg cactggcacc ggaaaccaac ggtcatgttg
     3961 cagtcaaagc gtggcaggcg ctgggcgaga tcaccgggcg cgaacatacc catctggcgc
     4021 tgcacaaaga ggacgagaag attcgctttc gcgatattca ggcgcagccg cgtaaaatta
     4081 tctccagccc cacatggtcc ggtctggaaa gcgatcacgt atcctataac gcgggataca
     4141 ccaacgttca tgagttaatt ccgtggcgca cgctgtcggg acgccagcag ctctatcagg
     4201 atcatccgtg gatgcgtgct tttggtgaaa gcctggtggc ttatcgcccg cctatcgaca
     4261 cccgtagcgt cagtgagatg cgccagatac cgccaaacgg cttccaggaa aaagcactta
     4321 acttcctgac gccgcaccag aaatggggca ttcactcaac ctacagtgaa aacctgctaa
     4381 tgctgacgct ctctcgcggt ggaccgattg tctggatcag cgaaacagat gcccgagaac
     4441 tgaccattgt cgataacgac tgggtggaag tatttaacgc caacggcgcg ctgactgccc
     4501 gcgcggtggt cagccaacgt gtaccgccgg gcatgaccat gatgtatcac gcccaggaac
     4561 gcattatgaa tattcctggt tcggaagtaa ctggcatgcg cggcggcatt cataactcgg
     4621 tcacccgcgt ttgcccgaaa ccgacgcata tgattggcgg ttacgcgcag ctggcctggg
     4681 gctttaacta ctacggcacc gtcggctcga accgcgacga attcatcatg atccgcaaga
     4741 tgaagaacgt taactggctg gatgatgaag gtcgcgatca ggtacaggag gcgaaaaaat
     4801 gaaaatacgt tcgcaagtcg gcatggtgct taacctcgat aaatgtatcg gctgccatac
     4861 ctgttcggtg acctgtaaaa acgtctggac cggacgcgaa ggcatggagt acgcatggtt
     4921 taacaacgtc gaaaccaaac cgggcattgg ttatccgaaa aactgggaag atcaggaaga
     4981 gtggcaaggc ggctgggtcc gcgatgtgaa tggcaagatc cgtccgcgtc tgggcagcaa
     5041 gatgggcgta ataaccaaaa tcttcgccaa cccggtggtg ccgcagcaga ttgatgatta
     5101 ctacgaacct ttcaccttcg actacgaaca tttgcatagc acaccggaag gcaaacatat
     5161 ccctactgct cgcccgcgtt cactgattga cggcaaacgg atggacaaag tgatctgggg
     5221 cccaaactgg gaagaactgc tgggcggcga gtttgaaaaa cgtgcccgcg accgcaactt
     5281 cgaggccatg caaaaggaga tgtacgggca gtttgaaaac accttcatga tgtacctgcc
     5341 gcgcctgtgc gaacactgcc tcaatcccag ctgcgtggcg acctgcccaa gcggcgctat
     5401 ctacaaacgc gaagaagacg gcattgtgct gattgatcag gataaatgcc gtggctggcg
     5461 tttgtgcatt agcggttgtc cgtacaaaaa aatctacttc aactggaaaa gcggcaagtc
     5521 agaaaaatgt atcttctgtt atccgcgaat tgagtcaggc caacccaccg tgtgctcaga
     5581 aacctgcgtg ggtcgcatcc ggtatctggg cgtgctgctt tacgacgccg accgcatcga
     5641 ggaagcggcg agcaccgagc gcgaagttga cctc
//
LOCUS       DBKEKI010000213         5446 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.215, whole genome shotgun sequence.
ACCESSION   DBKEKI010000213 DBKEKI010000000
VERSION     DBKEKI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.215"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..557
                     /gene="msrP"
                     /locus_tag="ACUQPX_004074"
     CDS             <1..557
                     /gene="msrP"
                     /locus_tag="ACUQPX_004074"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HHV9058553.1"
                     /translation="SMVVPWIGFPLHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDR
                     FIGGGLKYPYVEGLRLDEAMHPLTLMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKS
                     IVSIKLTRERPPTTWNLAAPDEYGFYANVNPHVDHPRWSQATERFIGSGGILDVQRQP
                     TLLFNGYADQVASLYRGLDLRENF"
     gene            558..1193
                     /gene="msrQ"
                     /locus_tag="ACUQPX_004075"
     CDS             558..1193
                     /gene="msrQ"
                     /locus_tag="ACUQPX_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HHV9058554.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            1451..2101
                     /gene="zinT"
                     /locus_tag="ACUQPX_004076"
     CDS             1451..2101
                     /gene="zinT"
                     /locus_tag="ACUQPX_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HHV9058555.1"
                     /translation="MAIRLHKLAVALGVFIVSAPTFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2771..2860)
                     /locus_tag="ACUQPX_004077"
     tRNA            complement(2771..2860)
                     /locus_tag="ACUQPX_004077"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2824..2826),aa:Ser,seq:cga)
     gene            2954..3751
                     /gene="mtfA"
                     /locus_tag="ACUQPX_004078"
     CDS             2954..3751
                     /gene="mtfA"
                     /locus_tag="ACUQPX_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HHV9058556.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEIAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3852..3927
                     /locus_tag="ACUQPX_004079"
     tRNA            3852..3927
                     /locus_tag="ACUQPX_004079"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3885..3887,aa:Asn,seq:gtt)
     gene            4089..5351
                     /locus_tag="ACUQPX_004080"
     CDS             4089..5351
                     /locus_tag="ACUQPX_004080"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9058557.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1441 a         1270 c         1254 g         1481 t
ORIGIN      
        1 ggtcgatggt ggtgccgtgg attggttttc cgctacacaa attgctggcg cttgccgaac
       61 ccaccagcaa tgcgaagtat gtcgctttcg aaacgattta tgcgccggaa cagatgcctg
      121 gccagcagga ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa ggattgcgtc
      181 tcgacgaagc aatgcatccg ctcacactga tgaccgtagg tgtttatggc aaggcgttac
      241 caccgcaaaa tggcgcgccg gtgcgactga ttgtgccgtg gaaatatggc tttaaaggga
      301 ttaaatcgat cgtcagtatt aagctgaccc gcgagcgtcc gccaaccacc tggaatctgg
      361 cagcgcctga cgaatacggt ttttacgcca acgttaatcc gcatgttgat cacccgcgct
      421 ggtcacaggc taccgaacga tttattggtt caggcggcat tctcgatgtt cagcgccagc
      481 caacactact gtttaatggt tacgccgacc aggtggcatc gctgtatcgt ggcctggatt
      541 tgcgggagaa tttctaaatg cgtctgacag caaaacaggt gatatggctg aaagtttgcc
      601 tgcatcttgc cggattgttg ccgtttctct ggctggtctg ggcgatcaat cacggtggac
      661 tgggtgccga tccggtgaaa gatattcagc attttaccgg tcgcactgca ctgaaatttt
      721 tgcttgcggc cttgttaatc acacctctgg cacgctacgc aaaacagccg ttattgatac
      781 gcactcgccg cctgttaggc ttatggtgct ttgcctgggc gacactgcat ttaaccagtt
      841 acgcattgct ggagttgggc gtgaataatc tggcgttatt aggtaaggag ttaattaccc
      901 ggccttattt aacgttagga attatcagct gggttatttt gcttgcttta gcgttcactt
      961 caacccagtc gatgcagcga aaactcggca aacactggca acagttgcat aacttcgtct
     1021 atcttgtcgc gatcctggcc ccaatacatt atctgtggtc agtgaagatt atttcaccgc
     1081 agccactcat ctacgctggg ctggctgtac tgcttttagc cttacggtat aagaagttgc
     1141 tttccctttt caaccggtta cgcaagcagg cccacaacaa attatctcta taacttcata
     1201 ttgccgacaa acttcgcgtt atcccggccg gatgcggcgc gagcgcccta tccggcccac
     1261 acagatcgca tcaagttatg tttcgtttgt catcaacctt tcatcaccag aataatttat
     1321 tttatttcaa taagatagtt caatacacct gagaatacca tgctctcatt tcctaatgag
     1381 ttgttgcatt ttttcatatg ttacaatata acattacaca taatgtacat taactctgga
     1441 ggaaattgtt ttggcgattc gtcttcataa actggctgtt gctttaggtg tctttattgt
     1501 tagcgctcct accttttcgc atggtcatca ttctcatggc aaacccttaa cagaggtcga
     1561 acaaaaagct gctaatggtg tttttgatga taccaatgta caaaaccgaa cgctcagtga
     1621 ctgggatgga gtctggcaat ccgtttaccc tttactgcaa agtggcaaac ttgatcccgt
     1681 ctttcagaag aaagcggatg cagataaaac caaaacattt gctgaaatta aagattatta
     1741 tcgcaaaggt tatccgacag atatcgaaat gattggcatt gaggacggca ttgtcgaatt
     1801 ccatagaaat aatgaaacaa catcctgtaa atatgattac gatggataca aaatactcac
     1861 ctataaatca ggcaagaaag gcgttcgtta cttattcgaa tgtaaagatc ctgaaagcaa
     1921 agcccctaaa tatatacaat ttagcgatca tataattgca ccacgaaaat catctcattt
     1981 tcacattttt atgggtaatg attcccagca atctttattg aatgaaatgg aaaactggcc
     2041 aacgtattat ccctatcagt tgagtagcga agaagtggtc gaggagatga tgtctcattg
     2101 atttttttgc gctgacttca gttatttaac agaacagtgc ctggtagtaa caacaataat
     2161 ttaccaagac aaataagtaa gacaaactca gacacgctca tacttgagtc gtgtcttttt
     2221 tattatgtct gtagaaaaaa actgagaaaa aggcgaacaa taaatacaca actcagccat
     2281 acagactggc agcgcaaatg tttccaggag caagtcatct gtcatggtaa agctaaccag
     2341 aaatataggt taattttggt gtttaaacct tttttacttg ttagtatgat acaaaggctt
     2401 tcaaaaaagc tgggaaattc aacaactctt ggaaattatg gactttgtcc cgcggcttta
     2461 atctgacggc cgcgttcctt ttttggctca attattgacc ccttctctac gcaacttcag
     2521 ttcccgccag caactttgcg gcagtttcat aggatcaatg tctaaaagaa taatggtgac
     2581 cgataagaaa acgactgaat aactgtagat tttcgctcga aaccttcctg tcagctccat
     2641 agcgaatcaa gcgctgaatg tcacagtatc gaacagaaaa cagtgacgat ctaacccttc
     2701 aagaatattc tacgattgtt ctgtttagga aaagcaaggc gggaagtcgg gagataagtc
     2761 attgataaag tggcggagag agggggattt gaacccccgg tagagttgcc cctactccgg
     2821 ttttcgagac tggtccgttc agccgctccg gcatctctcc gttcagatgg ttgccatgat
     2881 gccaggaaat ttggcatttt aacagtccct gtccgtgcaa ttttgttcaa gtgacgagtt
     2941 tgcgagcaaa acgatgatta agtggccctg gaaagtacaa gaatcagcac atcaaactgc
     3001 ccttccctgg caggaagcac tatcgatccc ccttttaacg tgtctgacag aacaggaaca
     3061 aagcaaattg gtcgctcttg ccgaacgttt tttacagcaa aaacggcttg ttcctttaca
     3121 gggctttgag ctgaattcat taagaagctg ccggatagca cttctatttt gcctgcccgt
     3181 tctggagtta ggactggaat ggctggatgg ttttcatgaa gtcttaattt atcctgcgcc
     3241 atttgtggtc gatgatgaat gggaagacga tatcggtctg gtgcataacc aacgtattgt
     3301 tcagtcaggt cagagctggc agcaagggcc tatcgttttg aactggttgg atatacaaga
     3361 ttcttttgat gcttctggtt ttaacctgat tattcatgaa atcgctcata agctggacac
     3421 ccgtaacggc gatcgcgcca gcggagttcc ctttatttcg ttgcgtgagg ttgctggctg
     3481 ggaacacgat cttcatgctg caatgaacaa cattcaggaa gaaatcgaat tagttggtga
     3541 gaatgcggcg agcattgatg cttatgctgc cagtgatcct gctgaatgtt ttgccgtact
     3601 ttctgaatat ttctttagcg ccccagaact ttttgctcct cgtttccctt cattgtggca
     3661 acgtttctgt caattttatc aacaagatcc tttgcagaga ctgcatcacg ctaatgatac
     3721 agactcgttt tcggcgacga atgttcatta attaacaact ttgcagatta attaaccaat
     3781 tgaaatggct tatgaaattt agtgttgaca gacaaggtac cgctaagtaa tatgcgcccc
     3841 gttctcacga ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca
     3901 ctggttcgag tccagtcaga ggagccaatt ttctgttttc atgcatcctt gcgaatcttt
     3961 atctgatgtt gattcaacag gttagtgtga aaactcttct cggtgcattt tgattttacc
     4021 ctacgcatcg ggaaaaattg gtggtcaaat ctggggtcag gttagttcga taatggagtg
     4081 acccccatat atcccttacc gacgcaaaaa tccgcaccct caagccttct gataaaccct
     4141 ttaaagtctc cgattctcac ggtctgtatc tgctggtcaa gccgggtggc tcccgccact
     4201 ggtatctcaa ataccgtatt agcggtaaag aatcccgcat tgcgctgggt gcctatccag
     4261 ccatctccct gtctgatgcg cgacagcaac gtgaaggtat ccgtaaaatg ctggcgctga
     4321 atatcaaccc ggtacagcag cgggctgctg aacgtggctc acgaacaccg gagaaagttt
     4381 ttaaaaacgt ggcgctggcg tggcataaaa gtaacaggaa atggtcgcag aacaccgccg
     4441 accgtctgct tgccagcctg aacaatcaca tctttccggt catcgggaac ctacctgtat
     4501 cagaacttaa accccgtcat ttcattgacc tgctgaaagg gatcgaggaa aaaggtctgc
     4561 tggaggttgc gtcccgcaca cggcagcacc tgagtaacat aatgcgccat gcggtccatc
     4621 aggagttaat cgatacgaac cctgcagcaa accttggcgg cgtgaccaca cctcctgtca
     4681 gacggcacta tcctgccctg ccgctggagc ggctgcctga actgcttgaa cgtattgggg
     4741 catatcatca gggccgtgaa ctgacccggc atgccgttct gctgatgctg catgtgttca
     4801 ttcgctccag tgaactgcgt ttcgcccgct ggtcagagat tgatttcaca aaccgagtct
     4861 ggacgatacc cgcgacgcga gaacccatta ttggcgtgcg ttattccggc cgcggggcaa
     4921 aaatgcgaat gccgcatatc gtccccctct cagaacagtc catcgccatt ctgaaacaga
     4981 ttaaggatat caccggtaat aatgaactga tcttccccgg cgaccataac ccgtataagc
     5041 caatgtgtga aaacacggtc aataaggcac tgcgggtgat gggttacgac acgaaaaagg
     5101 atatctgcgg tcacggcttc cgggcaatgg catgcagtgc gctgatggaa tcgggtttat
     5161 gggcaaagga cgcagtagaa cgccagatga gtcatcagga gcgcaatacc gtgcgcatgg
     5221 cttatattca taaggcagag cacctggaag cccgcaaagc gatgatgcag tggtggtcgg
     5281 attatctgga agcatgccga gaatcttatg caccgcctta tacaattggt aaaaataagt
     5341 ttatcccata gtatactaac gaatcccaca ctctggcaaa ttaccaccac ctctacagga
     5401 ggtggttcta tatagacttt tatcaacttc atgacgaaaa acacct
//
LOCUS       DBKEKI010000214         5429 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.216, whole genome shotgun sequence.
ACCESSION   DBKEKI010000214 DBKEKI010000000
VERSION     DBKEKI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.216"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..359)
                     /locus_tag="ACUQPX_004081"
     CDS             complement(<1..359)
                     /locus_tag="ACUQPX_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHV9058558.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKE"
     gene            complement(356..1024)
                     /gene="tcyL"
                     /locus_tag="ACUQPX_004082"
     CDS             complement(356..1024)
                     /gene="tcyL"
                     /locus_tag="ACUQPX_004082"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HHV9058559.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1039..2025)
                     /gene="dcyD"
                     /locus_tag="ACUQPX_004083"
     CDS             complement(1039..2025)
                     /gene="dcyD"
                     /locus_tag="ACUQPX_004083"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HHV9058560.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2130..2930)
                     /gene="tcyJ"
                     /locus_tag="ACUQPX_004084"
     CDS             complement(2130..2930)
                     /gene="tcyJ"
                     /locus_tag="ACUQPX_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HHV9058561.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3018..3569)
                     /gene="fliZ"
                     /locus_tag="ACUQPX_004085"
     CDS             complement(3018..3569)
                     /gene="fliZ"
                     /locus_tag="ACUQPX_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HHV9058562.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPSTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(3615..4334)
                     /gene="fliA"
                     /locus_tag="ACUQPX_004086"
     CDS             complement(3615..4334)
                     /gene="fliA"
                     /locus_tag="ACUQPX_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HHV9058563.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNEREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(4499..>5429)
                     /gene="fliC"
                     /locus_tag="ACUQPX_004087"
     CDS             complement(4499..>5429)
                     /gene="fliC"
                     /locus_tag="ACUQPX_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HHV9058564.1"
                     /translation="TNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDTAKGTITIDG
                     SAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAANNTKATTIDI
                     GGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGYSVNGTTGAV
                     TKGVDSVYVDNNGALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTTDAETKAATT
                     ADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEV
                     SNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
BASE COUNT         1300 a         1422 c         1314 g         1393 t
ORIGIN      
        1 tcttctttcg gttcgccttt gacgatcacc ggcccttcaa taatgttttc cagcaccgta
       61 cgatgcggaa acaaattaaa gttctggaag acaaacccga cgtgctgacg caactggcga
      121 atcagcgatt tttgctgact taatgaacgt gcagtatcaa tagtgatatc gccaacggtg
      181 atcgttcccg cttccggttg ttccagcaga tttatgctac gtagcaatgt ggttttgccg
      241 gaaccactcg gaccgataat tgccaccact tcgccaggct ttacctcgag gtcgataccg
      301 tgcagcaccg tctgaccgtg gaattttttc accaggttct taacttcaat ggcactcatt
      361 ttggttctct ctcctggcga ttaagttgat tctcaaaatg gttctgcaac gtcgataaca
      421 cagtcgccat gatccagtag atgagcgaag ccgccaggta catggtgaac acctccagcg
      481 tgcgcgaggt aatcaactgc gcctgacgga acagctccgg cacctggatt gtcgcggcca
      541 gcgacgtatc ttttaccaga ctgataaagc tgttcgacag cggcggcaac gccacccgcg
      601 ccgcctgtgg caaaatcgcg cgacgcatgg tctgccacgg cgtcatacca atactggcag
      661 cggcttccca ctgaccttta tcaatagaag aaatggcggc gcgcagcgtt tcggcggcat
      721 aggcggcagt gttcagcgac agaccaatca ttgctgacgg aatgggatct aattcgatac
      781 caaactgcgg caggccgtaa tagatcataa acagttgtgc gatgagcggc gtaccacgaa
      841 aaatagagat ataaaaacgc gccagccagc gcacgggcca gattggcgac aggcgcatca
      901 gcgcgagaat aaacccgagt agtaagccaa aaaacatgcc gccgatactg agttgcaggg
      961 tatacccagc ccctttcaac aggaacggca aggaatcaat aaccagttgt atactttctt
     1021 gcatgagcgt tcccggagct aaacgtgggg atgataggcg aacagcgcag gcgcgccgcc
     1081 ggtatgaata aacagaatcg gcccttcatc tttgaagcgt ttctgactga taccgtcaat
     1141 cagccccgcc atcgcttttc cggtatacac aggatcaagc agaatgcctt ccagacgcgc
     1201 cagcaatttc actgcttcca tgccttcgtc gttcggcacg ccgtagccag gtgcaaaata
     1261 gtcatcccag agtaaaattt ccgctgatgc ggtcagctcc agttctttcg caatcgcctg
     1321 ttgtaggtta accactttcg gcaattgatc ggcaacggaa cgcgacacgg tcacgccaat
     1381 cagttcgctt tcaggcatca ggtgttccag cccaacagcc agtccggcgt gagttccggc
     1441 actgcccgat gcgactacca ccgacgaaat attaaccgcc ccttcacact gttgcgcgat
     1501 ttccagcgca ctctccacat aacctagcgc gccaagagca ttagaaccgc caaccggaat
     1561 gacatacggg cgaaagcctt gtgcttcgac tcgcgtcgcc agctcttcca gttgggcatt
     1621 gggatcggtc agtgcgtcgc acatttcaat ctgggtattg aacagatcca gcaacaaacg
     1681 attgccgttg gttaaatagt tttctgcggt tgtgccaata ggattttcca gcagcgccac
     1741 gcagtgcaga ccgagtttcg ccgcgactgc ggcagtctgg cgcacatggt tagactggat
     1801 cgcgccggca gtaatcagcg tatcggcacc ttcgcgcagg gcatctgccg cgagaaattc
     1861 cagcttacgt aatttattgc cgcccattgc catgggggtg acgtcatccc gtttgatgaa
     1921 aatttcccgt ccaagataat cagaaaagcg cggcagatat tcaagcggcg ttggcgcgcc
     1981 gataaactcc aaccgtggaa aacgggttaa attatgcagt ggcataacag cctccgatgt
     2041 gtgttgttgt gattttctta ttatgcacgc tgaaaacgcg taaataaaaa aggcgctagt
     2101 gaaagcgccc ttttttgtca ttatgctgat tatttggtca catcagcacc aaaccatttt
     2161 tcggaaagag cttgcagagt gccgtctttt tgcatttccg caattgcatc attcaccgct
     2221 ttcagcaggt cttcatttcc tttacgcagc gccacgccag actcctgacg ggagaatgct
     2281 tcaccggtta ctgccagcgt atcgttggtt ttcttcacca gatccagcgc cgccagacga
     2341 tcaacgagga tcgcatcgat acgccctacg cgcagatcct gatatttggt cgggtcatca
     2401 tcataggtac gcacatcgac accctgaaca ttctgccgca gccactcttc atagttggtg
     2461 cccagaccga cacccacttt tttgcctttc agatcagcag ccgttttaat ggtgccttcg
     2521 ttaccttttt tcaccagcgc ctgaataccg gaaatggtgt aaggggtaga gaaatcgtat
     2581 tttttcttgc gctcgtcaga aatggtgacc tgattaatca ccacatcaat gcgtttagag
     2641 tccagcgacg ccagcatacc gtcccatttg gtcggtttta gtgacgcctc aacgccaaga
     2701 tgttttgcca gctgttgggc aaattccact tcaaaaccgg ttaatttgcc gtcatccccc
     2761 tgaaaactga acggcggata agtgccttcc agccctacca gcagcgtgcc gcgctctttg
     2821 actttattaa gcagaccttc atctgcaaaa cttttaacgc tcatgcccgc aaccagcgcc
     2881 acggccatca cacccatcaa tgcctgacgt cccagatgtg ctaatttcat attcaccccg
     2941 aatgttgtta tgtctgtttg cagtgtagag ccattgtttg taaacacaaa aacaactccg
     3001 ctacatctta ttcttattta atatatatca gaagcaggca ggctggagga tttctgcaca
     3061 agtccggtac aggtttggcg ctgatagtgt tgatacttcc gtaatgcgat gcggtaattg
     3121 ttggtgctgg tggtgggcag ccacggggcg aggatttcat caaggaaacc gtcctgcagc
     3181 tgatcgaggg aaatattttg ctcatgtaga tgattaccca aacggcgcag acgaacgaca
     3241 tattcacgca ccgtacttgg gctcatttca gtttgctcaa aaagaaattt cttaaagccg
     3301 ataatatcga aaaaatcact ctgcgttttg caatgtaaat caccgcaaaa tcggcacaat
     3361 gccgcccatg attgttgttc cgtttgccag cctttttcat caagcagcgt gtccaggcgg
     3421 gaaatctcga ttttattcac tattttgccg tcgcgaacta aggtaatgcg atcgagtaat
     3481 ttacggcaat gcgcgcaatg ggtctggctg tgtttaaagt ctttaagata gcggcttaat
     3541 ggccgtcttt tcaggtgctg caccatcatt aagaactcct ggtagtcaaa gttaaagtgc
     3601 ggcatttact gacgttataa cttacccagt ttagtgcgta accgtttaat ggcctggctg
     3661 tgtaactgac tgacccgcga ttccccgacc tccagcaccg cgccaatctc tttgagattc
     3721 agctcttcct ggtaatagag ggttaatacc agtttttcgc gctccggcaa cgtttcgatg
     3781 gcttccatca cccgctggcg cagattactg tccagcagtt gttgtagcgg gttttctcgc
     3841 tgatgatcat cagtaaccag ttcgatgcta tcgccgtgct cttcacgcca ctcatcgtag
     3901 gagaagagct ggctgttatt ggtgtcgagc agcatttgac gataatcggc aatatcgatc
     3961 cctaaacgtt ccgctacctc agtttccgtg gcgttgcggc caagttcctg ctccagttgc
     4021 cctattgcct gtgccacttc acgctcgttg cgtcgcacgc tgcgcggcac ccagtcacgg
     4081 ctgcgaagtt catccagcat agcgccacgg atacgctgca ctgcgtaagt tgtaaatgcc
     4141 gttccttgta gggcgtcata gcgttcgaca gcattaagta acccaatgcc gcccgcctgt
     4201 agcagatcgt caagttccac gctcgcgggc agtcgaacct gcaggcgcaa tgcttcgtga
     4261 cgcaccagcg ggacataacg ctgccacagc gagtgtttat ccattacacc ttcagcggta
     4321 tagagtgaat tcacgataaa cagccctgcg ttatatgagt tatcggcatg attatccgtt
     4381 tctgcagggt ttttaatcgg acgattagcg ggtgaaatga ggggttattt gggggttacg
     4441 caaaaagccg aatgtaaaaa accccgccgg tggcggggtt tgagaggcat tcagacgatt
     4501 aaccctgcag cagagacaga acctgctgcg gtacctggtt ggcttttgcc agcacggagt
     4561 taccggcctg ctggataatc tgcgctttcg acatgttgga cacttcggtc gcatagtcgg
     4621 cgtcctgaat acgggactgc gcttcagaca ggttggtagt ggtgttgttc aggttggtga
     4681 ctgcggaatc caaacggttt tgcaccgcac ccagggagga gcggaatttg tcgatggagc
     4741 tgatggcttc atccagagct ttcaggggat cggctgtcgt agctgctttt gtttcagcat
     4801 ctgtcgtcaa tttaccatca gcatctttat aaactgcctt accgctgccg ttagtcactg
     4861 aaccatcatc ctgtaggtaa aaatctacag tatcagatgt ggtcaacgcc ccgttgttat
     4921 caacataaac cgaatcaaca ccttttgtta cagcgccagt tgtgccgttg acgctataac
     4981 ctgcagtagt gaaatcaaca ttgtttcccg aggttgatac agctttatcg aaagctgcct
     5041 gatcaacttt tgcacctgtt actgaataag taatagtgtc cggcgtagta ctattaccgg
     5101 taaaggagat agaggtaccg ccaatgtcaa tggttgtcgc tttggtatta ttggctgcaa
     5161 gtgtggacag actagcctca gtcaaagaag caccagagcc gtttttcgtt aagcgcccag
     5221 ttgtatcaat gtagagttta tctccattgc ttgccgtaat tttaccatca ctgctgatct
     5281 gaacatcctg tgcagaacca tcgatagtaa tagtgccctt agcggtatca ccaactcccg
     5341 gggtcaaata tttctgaaca tcggcagctg aagccgttgt ggtatcaaaa gagtatgact
     5401 tacttgcact gtcatacttg taattcgtt
//
LOCUS       DBKEKI010000215         5286 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.217, whole genome shotgun sequence.
ACCESSION   DBKEKI010000215 DBKEKI010000000
VERSION     DBKEKI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.217"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..411
                     /locus_tag="ACUQPX_004088"
     CDS             <1..411
                     /locus_tag="ACUQPX_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HHV9058565.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            437..742
                     /gene="istB"
                     /locus_tag="ACUQPX_004089"
                     /pseudo
     CDS             437..742
                     /gene="istB"
                     /locus_tag="ACUQPX_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(911..5005)
                     /gene="sepA"
                     /locus_tag="ACUQPX_004090"
     CDS             complement(911..5005)
                     /gene="sepA"
                     /locus_tag="ACUQPX_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HHV9058566.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSWADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1295 a         1330 c         1232 g         1429 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cggaagcgta tctgcgccat attctgagcg tactgccgga atggccctcc
      361 aaccgtgttg acgaactcct gccatggaac gtggttctca ccaataaata agcgtcaata
      421 cggtgctccg ttgacgctta cactgaatga gctgctactg aaactggatc gctaccgggt
      481 gatagtggtg gatgatcttg gctatgtcaa acgcgacagc gccgaaacgg gtgtgctgtt
      541 cgagttaata gcgcatcgct atgaacgtgg gagcctggtg ataaccagta accatccgtt
      601 cagcatgtgg ggcagcatct tcgtggatga gactatggcg gtggcggcag cagaccgact
      661 gatccatcac ggatatatgt tcgaactgaa aggggaaagc tacaggaaaa agacagcgaa
      721 ggcagtaaca agcgtgactt gatgtcgcac tgaaggggcg gtcagtatag ttggcgccag
      781 tcggcaaaac tagttgacgt ctaatacctg gacggtcact tctgagaaaa ggcatttaca
      841 cagaatctta aacaggctca cggagttctt tcagaaaaag gcctgccgaa tggcaggcct
      901 atcccattcg tcagaaaaca taacggaagt tggcgtttat cgcattatcc acattatatt
      961 tcccgaacgc tgatttctcc agctccagtc ccaaacgcat attatcctta atttccgcat
     1021 tcatccctac cgtcatcagc atcctgctat ctttttcacc ttcgaaacgt ttctctccgg
     1081 aagcatcctg cagaaccgtt tctccgttcg ccagcaggtc gaactgataa cccagcccgg
     1141 ctcgagctgt gattttccag tcgtctccgg agaaggctct tcccacgtca acaccagtac
     1201 ggccaatcag tgggttataa tccttgtctt tcatgctcag agccattccc cggtcttccc
     1261 agctaaaagc ttttcctgat acagaaccgt aaaccagctc tatctgtggc tccacccagg
     1321 actctttcgt caggtggtaa cgataaccaa cttcagcacc ggcataccag gaatgagagc
     1381 tgtagtcttt tgttcccagt gaggcaaaat tcgccgtgtg ctgattatca tgatggagat
     1441 atttaccaat caggtcaaaa taagctccgg aattaaagag tgcagaggca tacagaccgc
     1501 cacccacgga tttgtttttt ccactgaatg cgtggctgct tgcattgctg tccgtatagg
     1561 tcagcaatgc ccccgtaaat aaatccacac cgtccagctc atgctttctg tcgacaccaa
     1621 tctgaacgtg agtgtagtta tcgctgtagt caccatctgc cgaaccggta ccattcatta
     1681 tgcgtgccca gacaccggca tccccctgag tatcccgcag gtcacccata cgtttgttca
     1741 ggttattgac ctctgtaagg aaggacttat aacctgatgc cataaagtct tttgcggctt
     1801 tcgaggctcc ggcatcagcg gtagtctgat atcctgtcag cacccatttt gtggcatcat
     1861 ccgttttttc cgtgctgatt actggcgtaa cgttactgaa accaatctgc tgcgtaccgg
     1921 cagtgaacgt tttctcatca ctccctttcg gtgcggtaat taacgtcacg ctcatttcac
     1981 tggctggttt gtttaaaaag tcaaccagga cgctattatt actgcctgac agtttgtctg
     2041 tgacattcaa ctggtcagcc cagctgttgt tggtatgcat caggaacgtg gtattatctg
     2101 cagaaagctc ttttacttgt aaagtggcaa agtgtttccc gtcccccagt tcaattcgcg
     2161 agcccttagc cttcagtgca ccggcctctg attttccgtc agagtaccac agagcattga
     2221 tcatatttac cgtactctgt gcccccgtaa tactgcctct cagaacagcg ttatatccct
     2281 tcattaagga gctgaataac ggagagtccg tcttccctgc atcagcatca ctgtctccca
     2341 gattaatggt tgccgcagtg gttcctgcat gaatatccga actcagatag cccatatttt
     2401 taacagaaaa gctggcgtta tcttcgaggt taatcccttc tgttgtagaa attacgggtg
     2461 agtaataccc ctgtttctgc gcagaaggca tccccgtcag ggtcagagaa gcattttctt
     2521 ttacatccac agccccggtg ctgaaaatac cgctctgagc cgtaagtttt gctccttttc
     2581 cgagggtcag gctgctgttg acaaagctgc caacacggtc aaaaacagca ttctgagagg
     2641 tcacactcac ggtgctatca taagcctcaa taccaccggt gaatctctcc atgatttgca
     2701 gggatgattt attttccagc gtaacatttc cgctgtaatc actctggtca ccaactgtgg
     2761 ttgcagtggc actcccttca acagaggaga ccgcattacc ggttccgtca ctcctgtcag
     2821 taaagacacg gctacttccc agaatgactg tacttccatt ggtggcgtta atatctccct
     2881 gcatcttgcc gtttctggac agatggaatg ttgccgcatc gagtttcagt ttttccagcg
     2941 taaactgacg ggtttcccag tcgctctggc tggaagaagt cgtcgttcca gcatgaataa
     3001 ctggatgacc ctggaatatc agcgtgccgg acttcttggt tatcgaacct tctggcaggt
     3061 taactgaacc atcaagagcc agtacatcct tacctgagag ctgcggaata ttcacatcca
     3121 tattgccgtt tagttggccg tgatacatat aaggctggct gctggcctca attttttgtt
     3181 gcttcacagt atcaatggct ttattacgat cttttccgac attctgccag acactgctgt
     3241 tgttcagatc ggagaagaac ggggaatatg aactcgcttt caggatgaaa tattgcttgg
     3301 tagaactgtc ataatatagg tctcccggcg ctcctctccc cccaaaaata ctgacagtat
     3361 taaccggtac attgatgtca ctggctttta atgtctgaag gtcgagtgtt accgttgatt
     3421 ttttctcact gttattggta atgatggccc cgtagtcagc cgcctgaaga cgggtaaagg
     3481 tcagattatt accattcagt tccagacgtc ctccacggta tccccacgaa atattatccg
     3541 gattaacttg ctgtgagtca gaaagcacaa ctgtcggacg accgctggca ataccaacag
     3601 agctgaaagc ctgtaccttt ccgtcagcat ccgcctgctg gttgagaata acggtaccgt
     3661 cccccacctt gagtccgcct gcattcacac cggtcccgtt tactgtcagg gtaccttcgc
     3721 cagttttatg caggttatca ccggcaactc cgttcacctg ccacagaacg tgggttcctt
     3781 tatcggtgat aatcccgccc cccatccatg tctggccgtt cagggcggac accctgtaat
     3841 cgccggcaaa ctgcagatac ccagctccct gattaacgga attctgcaga acgacctcgc
     3901 cattattgcc ggtgaacagg aggttttttc ctgcgttcag atcatttcct ttctttccat
     3961 gcatatccca agtcgtgttt ccctgagtca gtgtgccggt gccattagcc gcatcatatt
     4021 tccactgcag tactccttca ccagaggtgt aggctatagt tttgtcaaaa tcattttgcg
     4081 gttgctgacc aaggaaatcc tgagtggtga cgacccagtt atttccatag ttagttcctg
     4141 aggaaagcac gccaatcagt acccattttt tttgcaggga atcataagca aacagggggg
     4201 agccgctgtc ccccatattc agataattag ccagaggtcc attcgcggga ttaaaaatgt
     4261 caccggtctg agctgtaatc atctgtccgt tgttatagga gttcagcgcg ctgacggttc
     4321 ccccaacaag aaatccgcca ttggtataca cttgggtgcg attaccattt ttgtctttga
     4381 cgtactgcat ccccccccca aggcgataga attcagtaaa gcgtccgccg gcctgatagg
     4441 caccgctcac agccccgatg tcagacactt ctgccggcgc cacctctgtc accagtttac
     4501 tcaggcggcg ggttttaata tcaagaccgc tattattatt tgtaccaacc gcagtatagg
     4561 tgtttttggc gtatccgaag ctcatcgtgt cagaaccgct gacatgtttg gcggtaacca
     4621 catactgagg ggagtaaagt gtatggtctc ccggaggcag gctaccggta gtaatagtgg
     4681 cactactgaa atcagccatc ggggctttat caagtttacc gaccagattg ccctgcttgt
     4741 cgtaaatgga aatattcgtg gtccccggcg taaactgacc tttattctcg gcaaagtcgc
     4801 ggaatatctg ataagggatc tctgcactga ccgttgctga cagagctggc catgccgaag
     4861 aaagtgataa agctgcaaca gacgtaagaa taacccgacg tgaaagtctg cgatggcttt
     4921 tgcaggtaac cctgcgggcc agttcggaga cagcaatcag gcttttggtt atatggcaat
     4981 acttaagata atatattttg ttcatataga aacatcaacc taattttgac aaagattaat
     5041 tacataggat gtagatggag cagtccgaga ttctactaat atcaattatt aagcatctgt
     5101 tttttagttg gtgtaatata atttttatta aggtaagcgg aaaaacaacc aaaaaaaacg
     5161 caagatgtta tttttttatc attttgagcc agtttggctg gggggatgct cttctcttaa
     5221 gcttcttgct caactttaaa aatagttatg ccagttcggt atcgataagg ggcagtttgg
     5281 atatcg
//
LOCUS       DBKEKI010000216         4994 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.218, whole genome shotgun sequence.
ACCESSION   DBKEKI010000216 DBKEKI010000000
VERSION     DBKEKI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.218"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..826
                     /locus_tag="ACUQPX_004091"
     CDS             <1..826
                     /locus_tag="ACUQPX_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9058567.1"
                     /translation="LLHGETLYLDNPRSNKKGRTVRTVDNYIALLCSLLRFAYQSGFI
                     STKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALA
                     WEDVDFEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKS
                     EITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRHNPY
                     HTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLDARLS"
     gene            1289..2203
                     /gene="sitA"
                     /locus_tag="ACUQPX_004092"
     CDS             1289..2203
                     /gene="sitA"
                     /locus_tag="ACUQPX_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HHV9058568.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDMVKKNHIPAVFSESTISDNPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            2203..3030
                     /gene="sitB"
                     /locus_tag="ACUQPX_004093"
     CDS             2203..3030
                     /gene="sitB"
                     /locus_tag="ACUQPX_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HHV9058569.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            3027..3884
                     /gene="sitC"
                     /locus_tag="ACUQPX_004094"
     CDS             3027..3884
                     /gene="sitC"
                     /locus_tag="ACUQPX_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HHV9058570.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            3881..4738
                     /gene="sitD"
                     /locus_tag="ACUQPX_004095"
     CDS             3881..4738
                     /gene="sitD"
                     /locus_tag="ACUQPX_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HHV9058571.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
BASE COUNT         1281 a         1174 c         1212 g         1327 t
ORIGIN      
        1 actgctgcat ggagaaacgc tttacctaga taatccaaga tccaacaaaa aaggaagaac
       61 cgtgcgcaca gttgataact atatcgccct gctctgttcg ttgttacgtt ttgcgtatca
      121 gtcgggattt atatcaacca aaccatttga aggagtaaaa aaattacagc gaaacagaat
      181 aaagcctgac ccgttatcta aaacagaatt caatgcatta atggaaagtg aaaaaggaca
      241 gagccaaaac ttgtggaaat ttgccgttta ctccgggctt cgtcacgggg aactggcagc
      301 tctggcgtgg gaggatgtgg atttcgagaa gggaattgtg aatgtcagaa gaaacctgac
      361 gatacttgat atgttcggtc ccccaaaaac aaatgccggg atccggacgg taacattact
      421 gcagccggct cttgaagcac tgaaggagca atacaaactg accgggcatc atcgcaaaag
      481 cgaaatcact ttttatcatc gggagtacgg cagaactgaa aagcaaaaac tgcattttgt
      541 tttcatgccc agggtgtgta acgaaaaaca gaaaccttat tactcggtaa gcagtttggg
      601 tgcaagatgg aatgcagcag taaaacgtgc tggtattcgc cgccataatc cgtaccatac
      661 gcgacatact tttgcctgct ggctgttgac ggcaggagcg aacccggcat ttatagccag
      721 ccagatgggg catgaaactg cgcaaatggt gtatgaaatt tacggtatgt ggattgatga
      781 catgaacgac gaacagatag ccatgttgga tgcgcggtta tcgtagttgc aaagtttgcc
      841 cccaatttgc cccatttagt accagagaac tgaaataatg caagaaaatc aacaaattac
      901 aaagaaagaa caatacaacc tgaacaagta agggcaaaaa tcacaactat ctgactcata
      961 agtattttat ttatttttca atatgttaaa cattatcagc gacaacaata agctacgata
     1021 acccactctt tttttgcccc ttttttgccc cttctacagc gttttgcccc atttttgcca
     1081 tcaacaattt tcctcataaa gtcatttcaa agcctaatag ctgctctact tttttcctca
     1141 atatcatgtc gccagcagga aacaacctaa tccctgtttt aggcctcaaa gctgttaatg
     1201 ataattattt tcattttgta taggttatgc tataaaacat agccgttgct atgtttgttc
     1261 taattttgct cactataggt actaaattat gcactcaata aaaaaagtaa ccatgctctt
     1321 ggggtgcctc gccctcacct gctcgatcgc atttcaggca agtgcaactg aaaaattcaa
     1381 ggtcattaca acatttacca tcatcgcgga tatggccaaa aacgtggctg gagatgctgc
     1441 agaagtctca tccataacca agcctggtgc agaaattcat gagtatcagc ctacccctgg
     1501 cgatattaaa cgtgcgcagg gggcacaact gattctcgcc aatggtatga atctggaatt
     1561 gtggttccaa cgcttttacc agcatctcaa tggggttcca gaagtaattg tctcttcggg
     1621 tgtgacgcca gtagggatca ccgaaggacc ctatgagggc aaacctaatc cccatgcatg
     1681 gatgtcgcca gataatgctc tgatttacgt cgataatatt cgtgatgcgt tgataaaata
     1741 cgatccggca aatgcacaaa cctaccaacg taatgccgat acttataaag ccaagattac
     1801 ccaaaccctt gcccccctgc gtaagcagat tacggaactc cctgagaatc agcgatggat
     1861 ggtcaccagt gaaggggctt tttcttatct cgcacgcgat ttggggctaa aagagcttta
     1921 tctgtggccg attaatgccg atcaacaagg aacaccgcag caggtacgta aggttgttga
     1981 tatggttaag aaaaatcata tcccggcagt ctttagcgag agtacgattt ccgataatcc
     2041 agcgcgtcag gttgcgcgtg aaaccggcgc acactacggc ggtgtgctct atgtcgattc
     2101 cctgagcaca gaaaacggcc cggtacctac gtatatcgac cttcttaagg ttactaccag
     2161 tacgctggta cagggaatta aagccgggaa aagggagaaa taatgatgca atctgcaggc
     2221 attgtcgtta atgatgttac tgtcacctgg cgtaacgggc acacagcact gcgcgatgca
     2281 tccttcacag taccaagtgg atctattgcc gctctggttg gggtaaacgg ttccggaaaa
     2341 tcgacgttgt ttaaagcgat tatgggattt gtgcgtctga ccagcggaaa gatatctgtt
     2401 ctgggtattc ccacgcgaca ggcgctacag aaaaacctgg ttgcctacgt ccctcagtca
     2461 gaagaggttg actggtcatt tcctgtactg gtggaagatg tggtgatgat gggccgctat
     2521 gggcatatgg gtatgttgcg tatagccaaa aagagggatc gtcagattgt caccgatgcg
     2581 ctggaacgtg tcgatatggt ggattttcgc catcgtcaaa tcggcgagct ttccggtgga
     2641 caaaagaaac gcgtctttct ggcgagagcg attgcacagc agggtgatgt gatcctgctg
     2701 gatgaacctt ttaccggcgt tgatgtgaaa accgaagcga aaatcatcag cctgttgagg
     2761 gagttacgcg cggaaggcaa aacgatgctt gtctcaacgc acaatctcgg ctcagtcacg
     2821 acattttgcg attatacggt catggtcaaa ggcaccgtcc tggcaagcgg gccgacagac
     2881 accactttta cagccgagaa cctggagctg gcttttagcg gcgtactgcg ccatgtcaca
     2941 cttaacggct cggaagaaag cattattact gacgatgaac gcccttttgt ggcacatcga
     3001 ccgtcagcgg tgcagaggga agcaagatga acgtgctact ggaacccttc agctatgaat
     3061 atatgctcaa tgcgatgtgg gtctcggcaa tggtcggcgg tctctgcgct ttcctgtcat
     3121 gctatctgat gctaaaaggc tggtcgctga ttggcgatgc gctgtcgcac tctattgtcc
     3181 cgggtgttgc gggggcatat atgctggggc ttccgttttc tctgggagcc ttcttttccg
     3241 gcggactggc agcgggcagc atgctgtttc ttaaccaacg cacccgctta aaagaagatg
     3301 ccatcatcgg cctgatcttc tcgtcctttt ttggcctggg gctgtttatg gtgtcattga
     3361 acccgacatc cgtgaacatt cagaccatcg tcctgggcaa tattctggca atcgatccgg
     3421 cggatatcct gcaactcact atcattggca ttctctcgat catcgtgctt tttttcaaat
     3481 ggaaagatct tatggtgacc tttttcgacg agaatcacgc ccgtgctatt ggattacatc
     3541 cgggcagatt aaaaatcctc ttcttcaccc tgctgtcggt ttcgaccgtt gcggcgctgc
     3601 aaacggttgg tgcctttctg gtgatctgtc tggtggtgac tcccggtgca acagcatggt
     3661 tgttaaccga tcgttttccc cgcctgctca taattgctgt aactattggc agtgtaacca
     3721 gtttcctcgg cgcgtgggtc agttactttc tggacggcgc gacaggtgga atcatcgtgg
     3781 tggcgcagac actgctgttt cttttagcat ttgtatttgc gcccacccat ggacttttgg
     3841 caaatcgtcg gcgtgcgcat aaagcgctgg aggatcgctc atgatggcat tattactgga
     3901 gccactgcag ttcaccttta tgagtcacgc actgctgatt tcactggtag tttccatacc
     3961 ctgtgcgctg ctgtcggtct ttctggtcct gaaaggttgg gcactgatgg gtgacgcgat
     4021 gagtcatgcg gtttttcctg gcatcgtgct tacctggatt ctggggttac ctctggctac
     4081 aggggcattc gtcgccggag tattctgcgc ggtggcgacg ggatacctga aagacaacag
     4141 ccgaatcaag caagatacag ttatggggat tgttttttct ggcatgtttg ccgcaggtct
     4201 gatcttgtat attgcaatta aaccagacgt acatcttgac catattcttt tcggcgacat
     4261 gctcgggata accatcggcg atataatcca gacggtgatt attgccgggc tggttacact
     4321 tgttattagc gtaaaatggc gggatttttt gctgttcagc tttgattatc aacaggcgca
     4381 ggtaagcggt ttgcatacga gatggctgca ttatgggctg ctgtgcatgg tttccctgac
     4441 cattgtggcg acgctgaaag cagtaggtat cattctctct atttctctgc tcattgcgcc
     4501 tggcgccatt gcagtactcc tgacacaacg ctttcatatt gcgctcctac tggcgactgg
     4561 catatcggta atagtgtcaa tgactggtgt ctggctgtcc ttttttatcg acagcgcacc
     4621 agctccgacg attgtcgtct tattcgcggt cgtgttcatc atgacgttta ccgttaccag
     4681 catcaacgca cgcacaaagg gaaacgccca tacacaggat ctgttatcac ccaattagca
     4741 caacatccga ggacagcatc gtccgccagc ctacagaagt ccggaaagcc atggaaggtc
     4801 gttgggagga ggtacgaatt ctatcatgca aaaaatacgt aaaatcgata acgcctagaa
     4861 atcattcaat actcgcacta tcgaaagttt accagccaac cgcggcacgt tcctgcatac
     4921 gacgtgccgc agcttctgtt attccacgat aaaattccta accgaattca aatccttcag
     4981 actattcagc tctt
//
LOCUS       DBKEKI010000217         4858 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.219, whole genome shotgun sequence.
ACCESSION   DBKEKI010000217 DBKEKI010000000
VERSION     DBKEKI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.219"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..229
                     /locus_tag="ACUQPX_004096"
     CDS             <1..229
                     /locus_tag="ACUQPX_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HHV9058572.1"
                     /translation="GGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKM
                     PEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            318..1778
                     /locus_tag="ACUQPX_004097"
     CDS             318..1778
                     /locus_tag="ACUQPX_004097"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HHV9058573.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKTAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1814..2017)
                     /gene="ydfZ"
                     /locus_tag="ACUQPX_004098"
     CDS             complement(1814..2017)
                     /gene="ydfZ"
                     /locus_tag="ACUQPX_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HHV9058574.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2195..2881)
                     /gene="rspR"
                     /locus_tag="ACUQPX_004099"
                     /gene_synonym="ydfH"
     CDS             complement(2195..2881)
                     /gene="rspR"
                     /locus_tag="ACUQPX_004099"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HHV9058575.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2970..3716)
                     /gene="ydfG"
                     /locus_tag="ACUQPX_004100"
     CDS             complement(2970..3716)
                     /gene="ydfG"
                     /locus_tag="ACUQPX_004100"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HHV9058576.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3853..>4858
                     /locus_tag="ACUQPX_004101"
     CDS             3853..>4858
                     /locus_tag="ACUQPX_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HHV9058577.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQT
                     W"
BASE COUNT         1219 a         1165 c         1245 g         1229 t
ORIGIN      
        1 tggtggtctt atcgcttccg gttttggtcc tatcctggcg ggtattttct gcaccatgac
       61 ggaatcctgg tatccgatcg ccattatgat catggcatat tcagtgattg gtttaatctc
      121 tgcgctgaaa atgccagagg tgaaagaccg tgatttaagt gcgctggaag acgctgcgga
      181 agatcaaccg cgtgttgtaa gagctgcgca accttccaga agtctgtaaa ccctcaatcc
      241 cttctcttac cggagagggg atttttattc atataacaaa acatatagct tgccatattt
      301 aaatttaagg aattgttatg ggaaataatt tgttatcagc aaaagcgaca ctccctgttt
      361 atgatcgtaa taacctggcc ccaagaattg ttcatttagg ctttggtgca tttcaccgtg
      421 cgcatcaggg cgtgtatgcc gatattcttg ctacagaaca tttcagtgac tggggatatt
      481 atgaggtcaa cttaatcggc ggcgaacagc aaattgccga tttacaacag caagataatc
      541 tttataccgt tgcggaaatg tcggccgatg cgtggacggc tcgcgtcgtt ggcgtcgtta
      601 aaaaagcctt gcacgtacag atggatggct tagaaaccgt gttggctgcg atgtgtgaac
      661 cgcaaatcgc gattgtctct ctgacaatca ccgaaaaagg gtatttccac tctccggcga
      721 ccggacagtt aatgctcgat cacccgatgg tcgctgccga cgtgcaaaat ccccaccagc
      781 cgaaaacagc aacaggggtg attgttgagg cgctggctcg ccgtaaaacg gcaggacttc
      841 ccgcatttac cgtcatgtca tgtgacaaca tgccagaaaa cggtcatgtt atgcgtgacg
      901 ttgtcacttc ctacgcgcaa gccgttgatg taaaactggc acaatggatc gaagataacg
      961 tgactttccc atcaacaatg gtggaccgta ttgtgcccgc agtgacagag gatacgctgg
     1021 cgaaaatcga acaacttacc ggtgtgcgcg atcctgctgg cgttgcctgt gaacctttcc
     1081 gccagtgggt tatcgaagat aactttgtag ccggacgtcc ggaatgggaa aaagcgggag
     1141 ccgaactggt tagtgatgtg ctgccttatg aagagatgaa gttgcgcatg ctcaacggca
     1201 gtcattcatt cctggcatat ctgggttatc ttgctggata tcagcacatt aatgactgta
     1261 tggaagatga acattatcgt catgcggcgt atggcttgat gttgcaggaa caagcgccga
     1321 cgctgaaagt gcagggcgtt gatttgcaag attacgctaa ccgattaatt gcacgctata
     1381 gcaacccggc gttacgtcat cgaacctggc agattgcgat ggatggcagc cagaaattgc
     1441 cacagcggat gttggattct gttcgctggc atctggcgca tgacagcaag ttcgatctgc
     1501 tggcgctggg cgtcgcgggt tggatgcgtt atgtcggtgg tgttgatgaa cagggaaatc
     1561 cgatagaaat cagtgaccca ctgttacctg ttattcagaa ggctgtacaa agtagtgccg
     1621 aagggaaagc gcgcgtccag tcattgctgg cgattaaggc aatctttggt gatgatttgc
     1681 cagacaatag cttgtttact gcaaaagtga cggaagcgta cttgtcttta ttagcgcatg
     1741 gcgcgaaagc gaccgtggcg aaatattccg tgaagtaaaa aaagcaatgc cgccatcggc
     1801 ggcattcaca cacttagttc atgccgagac gaatcaggtc cagaggtgcc agtttctctt
     1861 cacaaccttc aacaactacg gtttttccgc ggcggatttg ctcagcggtc agaccttcag
     1921 tatcaatcga caggatcttg ccagtgtgac cggtgccgct aaccataaca cggctgccag
     1981 tggtgattgc gttacggtta cgatcgtagg tcgtcatagg tgttttctcc tttctgattt
     2041 acagtcgaag cccacgtaac gggcgcaata taaatactcc tgtcgagtga aatatttttt
     2101 gtttttgatc aagctcacat cttttttgat gcatgcatga gccgtaaggc ggggggaaaa
     2161 gcctctcatc ccggatggga tgagagtaaa tgaattactc ttcgctaaac cagtcgctgt
     2221 tttcctggcg gatctggcgc acggattcgc tgatttcctg caaatgttgc gtcattgcac
     2281 gttctaccgc atcgccatca cgtttttgca gggcagagaa aatatcaaga tgctggcgta
     2341 acagcatttc tggtggagaa acatggtcga aactcatata gcgcacgcga tcaacggtcg
     2401 ctttcaggtt ctcaatggta tcccacgcca gttgacagtc ggcaatctgc gtcaggagtt
     2461 gatggaagtt gtcatcaagt tcaaaaaaat catccagttg cttgcgctca atggcaatgc
     2521 gttgctggtg aagattttgt tccagttggt agcactggct ttcggtaatc atgctcgccg
     2581 cccgacgcgc caccgcgcac tcaatggcct gacggataaa actgccgttg cgcacctggg
     2641 ccatggaaat tttgttgacg tagctgccac gctgcggacg aatttgaatc aggccgtttt
     2701 ccgccagttt aataaaggct tcacgaaccg gctggcgtga cacattgaaa cgaacagaaa
     2761 cttctttttc cgacaacggt gtgcctggtg caatcaggca atggacaatg tcgcgacgaa
     2821 gaatacgata aatctgctga ttgacaggct gtgtgggatt aagttgcgtt tcgacggtca
     2881 ttcgttcttg cttgtgagtg agttaactgc atgagtctac cacttttttg tgacaagcaa
     2941 taacccggca gttacgccgg gtataaaaat tactgacggt ggacattcag tccggcatag
     3001 ctttgggtaa ccggcatcat ttccagggta ttgatattga cgtgagcagg cagcgttgac
     3061 acccaccaga cggcttcgct gacatcttct ggcgtcaatg caacggtatt ttgataggtt
     3121 ttttccgctt taccgtcatc gcctttaaag cggacattgg aaaactcggt gccacccacc
     3181 agacccggtt cgatgtcggt gacgcgcacc gccgtaccat gcagatccgt acgcagattc
     3241 aggctaaact gacgaacaaa cgctttcgtc gcaccgtaaa cgttaccacc ggcatacggc
     3301 cagctgcctg ccgttgagcc aatgttaata atatgaccat gattacgttc aaccataccc
     3361 ggtaagacgg cgcgcgtcat atatactagg cctttgttgt tggtatcaat catcgtttcc
     3421 cagtcttcaa cgctggcttt atgcgcaggc tccatgccca acgccaggcc ggcattattt
     3481 accaggatat caatattgct ccactcggca gggagcgatg ccagcatctc ttcaatagcg
     3541 gcgcggttgc gaacgtccag ttgggcgata tacagattat ctcccagttc gtcttttaac
     3601 tcctgcaacc gctcctggcg acggccagtg gcgataactt tatgcccttg ttgaataaaa
     3661 cgacgagtaa tgcattcacc aaaacctgcc gttgctccag ttactaaaac gatcatctca
     3721 ctgttcctca acgcttttgt gtgtcaataa catagcacgt gatgcttaag agcgggtaat
     3781 actggtttgc aagattgcac tccgttatca gtaagcctac gctatggaac acctcgcgtt
     3841 caggagaaga aaatgacaac aatgaatcct ttccttgtgc aaagcacact gccgtatctg
     3901 gctcctcatt ttgatcaaat tgccaatcat cactatcgcc cggcattcga tgagggaatg
     3961 caacaaaagc gggcagaaat tgctgccatc gcgcttaacc cgcaaacgcc tgatttcaac
     4021 aatactattc tggcactgga acaaagcgga gaattactta cccgcgttac cagcgtcttt
     4081 tttgcgatga ctgcggcgca taccaatgat gaattacagc gtcttgatga acagttttcc
     4141 gctgaactgg cggaactggc taatgatatc tatctgaacg gtgaattatt cgcgcgggta
     4201 gatgctgtct ggcagcgccg tgaatccctg gggcttgata gtgaatccat ccgcctggtg
     4261 gaggttattc atcaacgttt tgtccttgcc ggagccaaac ttgcacaagc tgataaagca
     4321 aaattaaaag tactgaatac agaagctgcg accctgacca gccagtttaa ccagcggtta
     4381 cttgcagcaa ataaatccgg cggtctggtt gtgaacgata tcgcgcagct ggcaggaatg
     4441 agtgagcaag agattgcgct ggcggcagag gcggctcgcg agaaaggtct ggataacaaa
     4501 tggctgattc cgctgctgaa taccacccaa caaccggcgc ttgccgaaat gcgcgatcgt
     4561 gcgacgcgtg aaaaactgtt tattgcgggc tggacgcgag cggaaaaaaa tgatgccaat
     4621 gatacccgcg ctatcattca acgtctggta gagattcgcg cacagcaggc gaaactgctt
     4681 ggttttcctc attatgccgc atggaaaatc gccgatcaga tggcaaaaac gccagaagca
     4741 gcactcaact ttatgcggga aattgttcca gcggcgcgtc aacgtgcgag cgatgaatta
     4801 gcctccatac aggcggttat cgataagcag cagggcgggt ttagcgcgca gacgtggg
//
LOCUS       DBKEKI010000218         4842 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.220, whole genome shotgun sequence.
ACCESSION   DBKEKI010000218 DBKEKI010000000
VERSION     DBKEKI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.220"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..240)
                     /locus_tag="ACUQPX_004102"
     CDS             complement(<1..240)
                     /locus_tag="ACUQPX_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058578.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLPRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLM"
     gene            755..1597
                     /locus_tag="ACUQPX_004103"
     CDS             755..1597
                     /locus_tag="ACUQPX_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HHV9058579.1"
                     /translation="MKKELFGRCPFDLDEDVKKIAAINKYIHAKFEEHKNKIDNLKGV
                     EQKLMVLQYFNVLNELSNQAISSLNIGAFSASEVLARVIIEQAANQFYIAIDDGENAQ
                     ALLKASKKLVHSNGKRWLECLKSKGMTNPAANERIRMGKELTDLFNRLWPNTPEYPGT
                     RKLFELIGWETHYHAHYVPLCDSIHTFSDDMVNIVSLYNAIQSDETTAIEFILAVKQE
                     NKRLAIYNFVIATALRCEALVNVFNSLGYQDIITEMTPTIDTVNQTIIRYDDFDHSRI
                     DDCQ"
     gene            1704..1967
                     /locus_tag="ACUQPX_004104"
                     /pseudo
     CDS             1704..1967
                     /locus_tag="ACUQPX_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            2102..2290
                     /locus_tag="ACUQPX_004105"
                     /pseudo
     CDS             2102..2290
                     /locus_tag="ACUQPX_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            2299..2442
                     /locus_tag="ACUQPX_004106"
                     /pseudo
     CDS             2299..2442
                     /locus_tag="ACUQPX_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            2594..3139
                     /gene="cobU"
                     /locus_tag="ACUQPX_004107"
     CDS             2594..3139
                     /gene="cobU"
                     /locus_tag="ACUQPX_004107"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HHV9058580.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDDGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            3136..3879
                     /gene="cobS"
                     /locus_tag="ACUQPX_004108"
     CDS             3136..3879
                     /gene="cobS"
                     /locus_tag="ACUQPX_004108"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HHV9058581.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            3891..>4842
                     /gene="cobT"
                     /locus_tag="ACUQPX_004109"
     CDS             3891..>4842
                     /gene="cobT"
                     /locus_tag="ACUQPX_004109"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HHV9058582.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQVGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLG"
BASE COUNT         1273 a          985 c         1261 g         1323 t
ORIGIN      
        1 catcagaggg atggcggcat gtccgggaag cactgtcatg agtgcactga ccggggtgac
       61 ctccccctga aacagtgcat tacagagtga ggatttaccg gccccgcttt tacccataat
      121 gccaggcacg ggttcgtgac gggggagttt gcgcagatgt tccaggatgt gacgggaaat
      181 cgggtaaggc agggaggaga gcggtttttc aattgcctca atggcatcag acggattcat
      241 acatgtattc ccagtgaaaa aagacaaaac cccgtgcacc ggaaaggtgg cgggggattc
      301 acggagataa tatctttctg aaattatttt gaatgcaggt atatgtggct gatttgtatc
      361 agctaatgag tacaggtctg aatctaatct gacagtccgc tttgtgccag gagcggactg
      421 acctgctccc cgatgattaa tacacagcta tgctagtaat gtctgtagat cgctgtgtgt
      481 tgcatctatc ggttgaactc acagtccaaa ccagactgtc agctttgatt agattctacc
      541 tactcaacct gtcagataag gtctgagcta atacatctaa tatatggctt tgtgcagggg
      601 acggctatcg cgaacgttag gctaggtatc tgaatacgca cagttcactt ggttgtactt
      661 cttgcactga tctgctatcc cgcacagcta tttataggta atcttcagtg tataatctgc
      721 cgacttatgg aagcttgtac aacaggagaa atatatgaaa aaagaattgt ttggacgttg
      781 cccgtttgat ctcgacgaag atgtaaaaaa aatagccgcg ataaataaat atatccatgc
      841 aaaatttgag gagcataaaa ataaaataga caacctaaag ggtgtggaac aaaaattgat
      901 ggtcttacag tactttaatg tactgaatga acttagtaat caggctatct cttctttgaa
      961 tataggagcc ttttcagcat cggaagtatt agctagggtt ataatagaac aggctgccaa
     1021 tcaattttat atagcaatag atgatggcga aaatgcacaa gcattactca aggctagtaa
     1081 gaaattagtt catagtaatg gaaaacggtg gctggagtgt ttaaaatcca aaggaatgac
     1141 taatccagca gcaaatgaaa ggattcgcat gggaaaagaa ctaactgatc tatttaatag
     1201 attgtggcca aatacacctg aatatccagg aaccagaaaa ctttttgaat taattggctg
     1261 ggaaacacac tatcatgccc attacgttcc cctttgtgat tcaatacata cattctctga
     1321 tgatatggta aatatagtat ctctatataa tgcaatccaa agtgatgaaa ctactgctat
     1381 cgaattcatt cttgcagtta aacaagaaaa taaacggctg gcaatttata attttgtaat
     1441 tgctacggcc cttcggtgtg aggccttagt aaatgtattt aactctttag gttatcagga
     1501 tattataaca gaaatgacac caacaataga tacagtcaac cagactataa ttcgatatga
     1561 tgattttgat cattccagaa ttgatgattg tcaataatta attgacagaa tacgtacatg
     1621 cgcaacaact agtgatagtt gacttatttt cgctcttgag agacaaccat actcaaatct
     1681 cccacattgc aggagatttg aatatgaaaa cgtcaccgtg gaataaagac cgtaccatcg
     1741 gtcaaaaaag gccacttcag atatctcata tctgggggat ccggattaga cttgagttgg
     1801 aaggtaaaac acgcgatcta gctctgttca acatggcttt ggacagtaag ctacggggct
     1861 gtgatttggt caaactcaaa gtatcagatg ctgcatatgg cagttctgtc tcaagcagag
     1921 caacggtgtt gcaacagaaa accggcagcc ctgtccaatt tgagatgcct ttaataacac
     1981 atcttttaaa tgttgaagaa ttatcccgcc tgctgaagaa cgttgctctg agtgtgataa
     2041 acctttttat tcttgatgat gtcagcgaga agatggaata tatccccgac tgggtcgtgg
     2101 ttacccggtt aaacatctgg tggcgctaat aaatctggca agtcacattc tgttgaatgc
     2161 tgtgacagtg ccttaccgag agagcgaaat tgtggtgttc tagtgcatag tcgccaccat
     2221 tccggataat tctattgcgt tgttttacaa gttcttttac agcgcagatc tgtcgctgac
     2281 gttgaatcaa tagcctggat ttcagaacac aatgcccgtt tgctggttgg tccggcagta
     2341 gcattaattc attatctgaa tgaaatgtgt caatccacag aaaccgctgt caatgacgca
     2401 ttttgtcgga cgttaacacg agaaagtctg gctattccgt aatgccatct tatttcgtaa
     2461 tggattgaga atgctggatt tttaattata ttacgcgcag tattaatcgc gtaaatgttt
     2521 tgcggtcagc aacggtgggg gatccattgc tctgttcaat gaataccatc atacatttga
     2581 atcaggagtc ggtatgatga ttttggtgac gggaggcgca cggagcggga agagtcgcca
     2641 cgcagaggtg cttattgggg actcttcaca ggttctgtat atcgctacct cgcaaatcct
     2701 tgatgatgag atggctgcac ggatagaaca tcatcggcaa agccgcccgg agcactggcg
     2761 cacagtggag cgctggcaac atcttgatga attaattcat gcagacatta acccgaatga
     2821 ggctgtgttg ctggaatgcg ttaccacaat ggtgactaat ctgttgtttg atgatggcgg
     2881 cgataaagac cctgatgaat gggattatca ggcgatggaa caggcgatta atgctgagat
     2941 tcagtcgttg attgctgcct gccaacgttg ccccgcaaag gttgtattag tgactaacga
     3001 agtgggaatg gggattgtgc cggagagtcg tctggcacga cattttcgtg atattgccgg
     3061 gcgggtaaat cagcagttgg ccgctgcggc aaatgaagta tggctggtgg tttcgggtat
     3121 tggagtaaaa atcaaatgag caaattattt tgggcgatgc tctcatttat tacgcgtctg
     3181 cctgtaccgc gtcgctggtc ccagggactg gatttcgagc attattctcg cggtattatt
     3241 acttttcctt tgattggatt attgcttggc gcgattagcg ggctggtctt catggtgctg
     3301 caggcatggt gtggcgcacc actggcggca ctgtttagcg tacttgtgct ggcgctgatg
     3361 accggggaat tccatctgga cggcctcgct gatacctgtg atggcgtatt ttctgcacgt
     3421 agccgcgatc gcatgttgga aatcatgcgt gatagccgtt taggcaccca cggcggtctg
     3481 gcattgattt ttgtggtact ggcaaagatt ctggtgttaa gcgagttggc tctgcgtggc
     3541 gaaccgatcc ttgcatcgct ggcggcagca tgtgcggtca gccgcgggac tgccgcatta
     3601 ttgatgtatc gtcatcgtta tgcgcgggaa gaagggcttg gcaatgtgtt cattggcaaa
     3661 attgatgggc gacaaacctg cgtcacactc ggcttagccg ctatttttgc tgccgtatta
     3721 ttgccgggta tgcatggtgt ggctgctatg gtggtgacga tggtggcgat tttcatcctc
     3781 ggtcagcttc tcaaacgtac gctgggcggg caaacgggcg atacgctggg agcagccata
     3841 gagcttggtg aactggtctt cctgctggca ctgctataaa gagacgtgac atgcaaataa
     3901 ttgccgattt actgaatacg atcccagcca tcaattctgc cgctatgtcg cgtgcacaac
     3961 ggcatgttga cgggttactc aaacctgttg gtagcctagg aaagctggag gcgcttgcca
     4021 tacaactggc aggaatgccg ggggtgaatg gcataccgca tgtgggcaaa aaagcggtac
     4081 tggttatgtg tgccgatcac ggcgtctggg aggaaggggt cgctatttct ccaaaagaag
     4141 tgacagccat acaggctgaa aatatgaccc gtggaacaac cggcgtgtgt gtgctggcag
     4201 cacaagtggg cgctaacgtc cacgtaattg atgttggtat tgatactgct gagcctatcc
     4261 ccgggcttat caacatgcgt gtcgcacgag gtagcggcaa tattgcttca gctccggcaa
     4321 tgagtcgccg tcaggctgaa aagttgcttt tggacgtcat atgttatacg cgggagctgg
     4381 caaaaaacgg tgtcacgctg tttggtgtag gtgaactggg gatggcaaac acgacaccgg
     4441 cagcggcaat agtcagcaca atcactggcc gggatcctga agaagtggtt gggattggcg
     4501 caaacctgcc gacagataaa ctggctaata aaattgatgt tgtgcgtcgg gcgattacgt
     4561 tgaatcaacc aaatcctcag gatggtgttg atgtcctggc aaaagtgggt ggatttgatt
     4621 tggtcggaat agctggagtg atgttaggtg ctgcttcctg cggtttaccc gtgttgctgg
     4681 atggatttct ttcttatgct gctgcgctcg cagcctgcca gatgtctcct gcaatcaaac
     4741 cgtatctcat tccttctcac ttgtcggcag aaaaaggcgc gcgtatagcg ctctcgcatt
     4801 tggggctgga gccttatctc aatatggata tgcgtttagg tg
//
LOCUS       DBKEKI010000219         4656 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.221, whole genome shotgun sequence.
ACCESSION   DBKEKI010000219 DBKEKI010000000
VERSION     DBKEKI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.221"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            39..773
                     /gene="ycgR"
                     /locus_tag="ACUQPX_004110"
     CDS             39..773
                     /gene="ycgR"
                     /locus_tag="ACUQPX_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0071945 - regulation of bacterial-type
                     flagellum-dependent cell motility by regulation of motor
                     speed [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein YcgR"
                     /protein_id="HHV9058583.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYNLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(775..1386)
                     /gene="emtA"
                     /locus_tag="ACUQPX_004111"
     CDS             complement(775..1386)
                     /gene="emtA"
                     /locus_tag="ACUQPX_004111"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HHV9058584.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            1486..2400
                     /gene="ldcA"
                     /locus_tag="ACUQPX_004112"
     CDS             1486..2400
                     /gene="ldcA"
                     /locus_tag="ACUQPX_004112"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HHV9058585.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            2495..4231
                     /locus_tag="ACUQPX_004113"
     CDS             2495..4231
                     /locus_tag="ACUQPX_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HHV9058586.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(4166..4303)
                     /locus_tag="ACUQPX_004114"
                     /pseudo
     CDS             complement(4166..4303)
                     /locus_tag="ACUQPX_004114"
                     /inference="COORDINATES: protein motif:HMM:NF049075.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4469..>4656)
                     /locus_tag="ACUQPX_004115"
     CDS             complement(4469..>4656)
                     /locus_tag="ACUQPX_004115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058587.1"
                     /translation="KENTRVNDPEIIVIKQRNNYKKSLMHPVLQADETPIVWAMSVRQ
                     VAWGNMFRRCQAGAPDG"
BASE COUNT         1038 a         1189 c         1193 g         1236 t
ORIGIN      
        1 taaaccaaag acagtttgtc agtcaggagt ttttccgcgt gagtcattac catgagcagt
       61 tcctgaaaca aaatccgtta gccgtcctgg gcgtgttacg cgatttgcac aaagccgcaa
      121 ttcctttgcg tctcagttgg aatggcgggc agctgatcag caaaatactg gcaataaccc
      181 cggataaact ggtgctggat ttcggcagtc aagccgaaga caacatcgcc gtgctaaagg
      241 cacagcacat taccattacc gccgaaactc agggtgcgaa agtcgagttt actgttgaac
      301 aactacagca gagtgaatac ttgcagcttc cggcatttat taccgtaccg cctcccacct
      361 tatggtttgt acaacgacgc cgatatttcc gcatctccgc ccctctccat ccgccttatt
      421 tttgccagac caaactggcg gataacagta cgttacgttt ccgcttgtat aatttgtcgt
      481 taggcggcat gggcgcatta ctggaaacag caaagcctgc cgaattacaa gaaggtatgc
      541 gcttcgctca aattgaagtc aacatggggc aatggggtgt ttttcacttt gacgcccagt
      601 taatctccac cagcgagcgc aaagtgattg atggcaagaa tgaaaccatc accactcccc
      661 gtctgagctt ccgttttctt aacgtcagcc cgacggtgga gcggcaatta cagcggatta
      721 ttttctctct cgagcgagaa gcccgggaaa aagcggacaa agtgcgcgac tgatttacat
      781 cgcgtccagt gcctgctcaa gtttgtagat atagcgcgga gcctgcggcg caggatgatt
      841 tcgcgctacg tgatcgagga actcgtcagc atccaaatcg ttgattttgc tgatcgcctt
      901 tttccgatct gacgagaaag tccgtagcag cgcacctgcc ccgttagcgt atgacaccac
      961 cagcgcatat tgcagtacct tcggatcttc aatgcctgcc agcgggccgg tttccagaat
     1021 attcaggtaa gccgccccca ttgaaatatt acgctctgga tttttcagct cgctggtcgt
     1081 cggctcacca ctccagccca tacggcgata aacatcacgt ccggaggttg aagcttttaa
     1141 ctgcatcaaa ccaatggcat tcgatttact caccgcgttc ggattaccac ccgattcgat
     1201 agcgataatc gccgtgatca attgtggatc gacgccccag gctgcaccgg ctttctggct
     1261 tattggcatc cactgcatcg cacgttgcac cggaactttc gcgttccacg gcgggttcgt
     1321 atagtcatgc tttgatgaac aacccgctaa taacacaatc aaaaaggcaa accatctcaa
     1381 tttcacttct tctatcctta tagcctggaa tcgccgttga ggtgacaacg gcacacctaa
     1441 gcggcatgat atgcatttcg tttctgttgt cagcaaggaa ttgccatgtc tctgtttcac
     1501 ttaattgccc catcgggtta cagcattaaa cagcacgccg cgctgcgtgg tatccagcgc
     1561 cttaccgacg cggggcatca agtcaataac gtcgaggtta ttgcccgtcg ttgtgagcgt
     1621 tttgcgggta cggaaacgga gcgtctggaa gatctcaatt cccttgccag actgactacc
     1681 cccaacacca tcgtgctggc tgtacgcggc ggttacggtg ccagtcgttt actggcagat
     1741 attgactggc aggcactggt ggctcgccaa caacatgacc cgttactcat ttgcggacat
     1801 agcgatttta ccgccattca gtgtggtctt ctggcgcaag gcaatgtcat cacctttagc
     1861 ggccccatgc tggtagcaaa ctttggtgcg gatgaactga acgcctttac cgagcaccat
     1921 ttctggctgg cgttacgcaa tgaaaccttc accattgaat ggcaagggga aggcccgaca
     1981 tgtcagaccg aaggcacgtt gtggggaggc aatcttgcga tgctgatttc actgattggt
     2041 acaccgtgga tgccaaaaat tgagaacggt attctggtgc tggaagatat taacgagcac
     2101 cctttccgcg tcgaacgtat gctgttgcag ctctatcatg cgggaatttt gccacgtcag
     2161 aaggcgatta ttctcggtag ctttagcggc agcacgccca atgattatga cgcaggttac
     2221 aacctggagt cagtgtacgc gtttttgcgt tcccgcctgt cgattccact cattaccggc
     2281 ctcgattttg gtcatgaaca acgcacggtt acgctgccgt tgggcgcaca tgccattctg
     2341 aataacaccc gggaaggcac tcagttgacg attagcggtc atcctgttct taaaatgtaa
     2401 aaaaatcacg cttcagggct aagtaaaacg ctgtctctgc cgctaatatg gtagggttta
     2461 tataagagac agcgtaatca ggagtaaccg accgttggat gccacaacaa taattagcct
     2521 tttcatctta ggatccatcc ttgtcaccag cagtatatta cttagttcat tttcttcccg
     2581 tcttggcatt cctattctgg ttatttttct ggcgatcggc atgctggcgg gagtcgatgg
     2641 cgtcggcggt atcccgtttg ataattaccc cttcgcctac atggtaagta acctggcgct
     2701 ggcgattatt ttgctcgacg gcgggatgcg cactcaggcc agttcctttc gtgtggcgtt
     2761 aggaccggcg ctgtcgctgg cgacgctggg cgtacttatc acctctggtt taaccggcat
     2821 gatggcggcg tggctgttta atcttgattt gattgaaggc ttattaatcg gcgctatcgt
     2881 cggctccacc gatgctgcag cggtcttttc tctgctgggt ggtaaggggc ttaacgaacg
     2941 tgttggctcg acgctggaaa ttgaatccgg cagtaatgat ccaatggcgg tctttctgac
     3001 gattaccctg attgcaatga tccagcagca tgaaagcagt gtcagttgga tgttcgtggt
     3061 cgatattctg cagcaatttg gcctcgggat tgtcatcggt cttggcggtg gttatttact
     3121 gctgcaaatg attaatcgca tcgccctgcc tgccggatta tatccattgc tggcattaag
     3181 cggcggtatt ttaatttttg ctttaaccac ggcgctggaa ggcagcggta ttctggctgt
     3241 ttatctgtgc ggttttctgc tgggtaatcg gccgattcgc aaccgctacg gcatcctgca
     3301 aaatttcgac ggcctcgcct ggctggcgca aatcgccatg ttcctggtgc tggggctatt
     3361 ggttaaccca agcgatctgc tgcctattgc cattccggcg ctcattttgt cggcatggat
     3421 gatattcttc gcccgtcccc tttcggtatt tgctggattg ctccctttcc gcggtttcaa
     3481 tctgcgtgag cgcgtgttta tcagctgggt gggattacgc ggcgcggtgc caatcatcct
     3541 ggccgtgttc ccgatgatgg cggggctgga gaatgcgcga ctgttcttta atgtcgcctt
     3601 ctttgtggtt ctggtttcac tgctcttgca gggaacatca ctctcgtggg cggcgaaaaa
     3661 agccaaagtg gtggttccgc ccgtcggacg tccggtgtca cgcgttggcc tggatattca
     3721 tccggaaaat ccgtgggagc agtttgttta tcaattgagt gctgataaat ggtgtgtagg
     3781 tgcggcactg cgcgatttgc atatgccaaa agagacgcgt attgcagccc tgtttcgtga
     3841 taaccagttg cttcatccta ccggcagcac ccgactgcgc gaaggcgatg tattgtgtgt
     3901 aattggtcgg gaacgcgatc tcccggcgct cggtaaactg ttcagccagt cgccaccggt
     3961 cgcgctggat caacgcttct ttggtgactt cattctcgaa gccagcgcca aatatgctga
     4021 tgttgcgctg atatatggcc tggaagacgg gcgagaatat cgtgataaac agcaaacgct
     4081 gggtgaaatt gtccagcagt tgctaggcgc ggcaccggtt gtcggtgacc aggtagagtt
     4141 tgccgggatg atctggacgg tggccgagaa agaagataat gaagtgttga agattggcgt
     4201 tcgggtggct gaagaggaag ctgaatctta aatttaatgc agcatctatc gccggatgcg
     4261 atgctttact gaacgcagcc tgagcctgta ataaacttgt catttcctga ttttgatatg
     4321 ctcatcccaa catcagaaga aggttaaatt atggatgaca aaccattgca ggctctggct
     4381 aatgaactca aaacaccggc tcaagaaagt cagcgtaagg gctttttccg actgtaacag
     4441 gtaataaaaa ccagccccgc attaccggtc aaccatcagg ggcccctgcc tgacatcgcc
     4501 tgaacatatt cccccacgca acctgccgca ctgacatagc ccacacaata ggcgtttcat
     4561 ccgcctgtag caccggatgc atgagcgatt tcttatagtt attcctttgc ttaataacga
     4621 ttatttccgg gtcgttcact ctcgtatttt ctttaa
//
LOCUS       DBKEKI010000220         4627 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.222, whole genome shotgun sequence.
ACCESSION   DBKEKI010000220 DBKEKI010000000
VERSION     DBKEKI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.222"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..736)
                     /gene="yeeO"
                     /locus_tag="ACUQPX_004116"
     CDS             complement(<1..736)
                     /gene="yeeO"
                     /locus_tag="ACUQPX_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HHV9058588.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVL"
     gene            897..972
                     /locus_tag="ACUQPX_004117"
     tRNA            897..972
                     /locus_tag="ACUQPX_004117"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:930..932,aa:Asn,seq:gtt)
     gene            complement(1010..1960)
                     /gene="cbl"
                     /locus_tag="ACUQPX_004118"
     CDS             complement(1010..1960)
                     /gene="cbl"
                     /locus_tag="ACUQPX_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HHV9058589.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2062..2979)
                     /gene="nac"
                     /locus_tag="ACUQPX_004119"
     CDS             complement(2062..2979)
                     /gene="nac"
                     /locus_tag="ACUQPX_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HHV9058590.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3307..3382
                     /locus_tag="ACUQPX_004120"
     tRNA            3307..3382
                     /locus_tag="ACUQPX_004120"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3340..3342,aa:Asn,seq:gtt)
     gene            complement(3438..4373)
                     /gene="ldtA"
                     /locus_tag="ACUQPX_004121"
     CDS             complement(3438..4373)
                     /gene="ldtA"
                     /locus_tag="ACUQPX_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HHV9058591.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLIVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(4435..4554)
                     /gene="cobT"
                     /locus_tag="ACUQPX_004122"
                     /pseudo
     CDS             complement(4435..4554)
                     /gene="cobT"
                     /locus_tag="ACUQPX_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="catalyzes the synthesis of
                     alpha-ribazole-5'-phosphate from nicotinate mononucleotide
                     and 5,6-dimethylbenzimidazole; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
BASE COUNT         1272 a         1186 c         1016 g         1153 t
ORIGIN      
        1 ccagcaccca caaaattgca actgcgccaa tataacgaga aatggttaaa cccaacccag
       61 ccccgacaaa tcccagtccc ggccaggaga aaaggccgta aatcaatatg ccgctaataa
      121 taatattaag aatattcagg ctaccgttaa tcagtagcgg tattttcgta ttccctgcac
      181 cacgaagtgc cccgctacca ataagagtga tggcagcggc tggataactg agtacggtca
      241 gctccagata agtcaacgcc agtgctttaa cttctgtcgt ggcatcaccc gcgacgaaat
      301 caataatttg ttcgccaaaa tgatgaatta gcgttgccaa cagtacggca aacaacgtca
      361 tgatcaccaa tgactgccgc gtcgccaccc tcgctcgtcg tcgatcccgc ttaccgagac
      421 taaatgctac aacgacagta gtaccaagat cgatagctgc aaaaaaagcc ataatgacca
      481 tattgaagct gtccgccaac cccacgccgg ccatcgcatc ttttcccagc cagctgacca
      541 gaaaagtgct caggaccccc atcaacagga cacaggcgtt ctccatgaag ataggaacag
      601 caagcggggt tatctcgcgc cagaacaaca ctttgtagct cttgcgttta gagtgccagc
      661 gagtgccgtg aacaacctgg cgtaaagcag aggagatatt caaagccgac cttaattgca
      721 gaaagtgaaa ccacatttca aatgatgagg gagaatcagc aaagctgcaa agattttcgc
      781 caacaaattg tctgcaaatg caacaaactg ttgatagaaa cggcaaacag ttgggaaatt
      841 taaaaatcgg gtttgacaaa atatttttcg ccgttaagat gtgcctcaac agcgattcct
      901 ctgtagttca gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag
      961 tcagaggagc caaatttctg aaaagcccgc ttttatagcg ggctttttgc tatatctgat
     1021 aatcaatttc ctcttcactg ttttccatca cctggcgctt gatatcctca actgacagtc
     1081 ctgcattaca aagttccaga aagcgccaaa catagttacg ctgaagttgt cctcgcttca
     1141 gtcccaacca gacagtatta gcatcgaaaa gatgccgcgt atccaggcgg attaaattct
     1201 cttcctcttg ttcgccgctg gattgttcgg caactaatcc gatcccaagc ccaagagcaa
     1261 cataggtttt aatgacatca gaatcctgtg cacttaatac aatatctgcc ggcaaacctt
     1321 tgcgggcaaa tgcgtcatca gtgcgtgagc gccccgtaat tccctgtcgg taagtaatta
     1381 acggccactt cgctattgat tccagcgtca atggtgtaat ttgcgttaag ggatgatcga
     1441 gtggaacaag caaactatgg tgccaacaaa accacgggaa ggcgacgagc tgcgggtcat
     1501 tactcaaacg ctcgctggcg ataccaatat cagcttcgcc attttgcaac aacgtcgcaa
     1561 tttcctgtgg cgaaccctgg attagctcga gccgaacctc cgggaaaagt tcgcgaaaag
     1621 ctttaatgac ctctggcaag ctataacgtg cctgagtatg cgtcgttgca atagtgagaa
     1681 caccagacgt atcgttggta aacaggtctg caagccgacg aacattactg gcttcattca
     1741 gaatacgttc tgcaatgacc agtaatgctt tgcccggttc agtcatgccc agcagtcgct
     1801 tacctcgtcg aacaaatatt tcgatgccaa gttcatcctc cagttcccga atatgacggc
     1861 taacgcctga ctgtgaggta aaaagcatat tcgcaacctc tgtcaggttg taatcctgac
     1921 gtgcagcctc gcggattatc tttagttgtt ggaaattcac ggtaaactcc gggcagttca
     1981 gatatcccgt tattgttaaa gtctaatgcc cggcataaca aataataaaa acccgcatct
     2041 tattccatcc cgatataacg cttagctcac caattgccac tgcctttttt ccatcactgg
     2101 agaactaatc actgacatta acaactcttt cactgcctgt gcctgtggtg acaagtttgc
     2161 tctggcgggt aaatttaagg acaaagagag actcattgaa ggagtggtaa tgcgtgacat
     2221 ccacccattt actgcgccac ataacgaacg cgcagccgat tcaggtaata ctgcaacgcc
     2281 catgccgctg gcaatcgctg cggtaagcgt ggcaatagac tcaatttcac caatgacttt
     2341 tgccgtgagc cgccgtaggg aaaaagcctc atcaacacga agtctaacag cgctgtaatc
     2401 actggggaga aaaaggttca tttgcgcaat agcattaaca tcaacgtttt gccccgggca
     2461 atcttgagtt cctaccagga aaagatcttc tttcagcaaa gcctgactgg ataccccagc
     2521 cacaggggaa tgctcataaa tcaccgccat atcgagttgg tgatttatca gtttttcgtt
     2581 aagcactgca ccactatttt catgaagata gataacgacc tccgggaatt cagcgcgaac
     2641 cgcctgtaat aagggcatgg tgatggatga cgcagcggtt cctggcgcaa agccaatcga
     2701 gacttgcccc gataatgact gaccaacgtt atgcaccgcc agttgggcct gctcacactg
     2761 acgtaaaatg gcccgtgcat gggtatagag aatttttccg gcgtctgttg gcgtaacgcc
     2821 ccgttttgtg cgaatcaaaa gctgttgatt taactcacct tccagtatgg caacctgctg
     2881 gctgagcgct ggttgtgcga tatgcaatac ttcagcagcc tgggtcaggc taccaatatc
     2941 tacaattttt acgaagtatt tcaggcgtct gaagttcatg ttgcctccgg tttttaagaa
     3001 tcggcccaag tgccgccatt acttacaacc agtatgcaag atgcctgcca gttctatttt
     3061 ggcgttgatg cgcaagctaa ccaactgtca aataagagat tatgatagat tcgtcattgg
     3121 ccctttttat cagcagtcgc attcacctgc cctataaaag gagggtatgc accacgatgg
     3181 ttcattaccc aataagattg aaagctcacc actttgttga aattggcagc aaacaaacaa
     3241 aaaaaatgca tttcaccctt tgacatcacc atgcactgcc attaatatgc gccccgttca
     3301 cacgattcct ctgtagttca gtcggtagaa cggcggactg ttaatccgta tgtcactggt
     3361 tcgagtccag tcagaggagc caaattcaaa aaagcctgct ttcgagcagg ctttttactt
     3421 tctaattacc aacgctctta aaacatctgt cttgaaccag aactaatttg cacaggcatt
     3481 cccgatcgat gttgcaacgc agcatttgcg cgatttacat caacttcttg cccgttgata
     3541 aacgcccgca aagatggggt taccggcaat ggcacttttc ggtcagactc atattctgca
     3601 cgattgcgcg acagcggctc atgaacttcc agccactttg aaccatccgg ttccgttgtg
     3661 tatttcactg gctgatcaat aatttgtaca cgagtgccta caggaacatt atcaaacaga
     3721 tatttgatat cgtcattacg cagacgaata cagccctgac ttacacggag cccaatacca
     3781 aaattggcat tggtaccgtg gatggcatac aacctgccaa tataaatcgc gtacagcccc
     3841 atggggttat cgggccccgc aggaacaaat gcgggcaaac tctcccctcg tttcgcatat
     3901 tcgcgccggg tgttcggcgt tggcgtccag gttggtgctt cttgtttacg ttcaacggta
     3961 gtcacccagt tacgcggggt ttctcgccca gcctggccga taccaatagg aaagacttcc
     4021 actgtattac tgtctggcgg atagtaataa agacgcattt cagcgacgtt aaccacaatc
     4081 cctttacgaa cagtggcggg caaaatcagt tgctgcggaa tggtgagttg tgagccaggc
     4141 ttcggcaaaa aaacatcagc gcccggattc gcttccagca tattacttaa cccttgcccg
     4201 tattgtgcgg caaaagtctc cagcggctga gtattgtgat caggaacagt tacaataagc
     4261 gactgcccca ctaaacggct accctctgga ggtaatggat aagttaccgc caggctattt
     4321 tggctggcaa aaagcagagc aaatgagcaa agaatattta cacgacgcat catgtccctt
     4381 tcctaagtcg cgaatactat ccgataagta tagcttttat cagacttttc gtttttagct
     4441 gttcaaatca gaagtcgtat tccccggtag aacaatatta ctggcagcaa gttcgcccat
     4501 gttgttgtat atcgcacaag cagcttcgat gatggacatc gccagagctg caccacgtta
     4561 ctttattcgt accccttata atggggtgtt agccagccag acccggcatg attactgccc
     4621 cccagtc
//
LOCUS       DBKEKI010000221         4612 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.223, whole genome shotgun sequence.
ACCESSION   DBKEKI010000221 DBKEKI010000000
VERSION     DBKEKI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.223"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(361..837)
                     /locus_tag="ACUQPX_004123"
     CDS             complement(361..837)
                     /locus_tag="ACUQPX_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HHV9058592.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(896..2185)
                     /gene="bioA"
                     /locus_tag="ACUQPX_004124"
     CDS             complement(896..2185)
                     /gene="bioA"
                     /locus_tag="ACUQPX_004124"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HHV9058593.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAVGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2272..3312
                     /gene="bioB"
                     /locus_tag="ACUQPX_004125"
     CDS             2272..3312
                     /gene="bioB"
                     /locus_tag="ACUQPX_004125"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HHV9058594.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3309..4463
                     /gene="bioF"
                     /locus_tag="ACUQPX_004126"
     CDS             3309..4463
                     /gene="bioF"
                     /locus_tag="ACUQPX_004126"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HHV9058595.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4450..>4612
                     /locus_tag="ACUQPX_004127"
     CDS             4450..>4612
                     /locus_tag="ACUQPX_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="methyltransferase; acyl carrier protein involved in
                     an unidentified step in the synthesis of pimeloyl-CoA, a
                     biotin precursor; member of the bio operon (bioABFCD); in
                     Escherichia coli, bioC-null mutants require biotin for
                     growth; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HHV9058596.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPG"
BASE COUNT         1089 a         1230 c         1273 g         1020 t
ORIGIN      
        1 gcatcatttc tctgcgctta tcgagatact gagcatggtt gtaaattcag ctaacccagt
       61 gcgttgacaa aaaattagcg caagaggaca aaaaatcacc ttgcgctaat gctctgtatc
      121 aggtcactaa taccatctaa gtggttgatt catagtgact ggatatgttg tgttttgcag
      181 tattatgcag tctatttttt aagctaaatg tattataatg cattgatatt aatgattttt
      241 actgtttctc gttcagcttt tttatactaa cttgagcgaa acgggaaggt aaaaagacaa
      301 aaagttgttt ttaatacctt taagtgatac cagatggcat tgcgccatct ggcagagtga
      361 ttaactaaac atcgcggtaa tcgatgcgct tgccagagag tggaaatgaa cgttaaaccc
      421 gaccatcgcg ccgctggctc cttcatcgac atcaatacgc tctacatcca gcgcgtgaac
      481 ggtaaaaatg tagcgatggg tttcgccttt cggcggcgct gcgccatcgt atccggtttt
      541 accaaaatcg gtacgcgtct gcaaaacgcc gtctggcatt gctaccagac cagagccaaa
      601 cccttgcggt aatacgcggg tatcagcggg taaattaaca actacccagt gccaccagcc
      661 ggagccggtt ggcgcatccg ggtcgtagca ggtgacaaca aaacttttcg ttcccgcagg
      721 aacatcatcc cacgccagat gcggtgaaat attatcgcca tcgtaaccca tgccgttaaa
      781 gacatgacga tgcggcaact tatcgccatc gcgcagatcg ttactgatga gtttcatgaa
      841 ccctcctttc ttgtttgcag aaagtgtagc cagaaaccct cacgctgact tcccgttatt
      901 ggcaaaaaaa tgtttcatcc tgtaccgcgc ggttaaccgc tgcggtcaga cgctgcaact
      961 gttgcgggag aataatatag ggcggcatca ggtaaatcag tttgccaaaa ggccggatcc
     1021 ataccccctg ttcgacaaag aatttttgca gcgccgccat attcaccgga cgagtggttt
     1081 cgaccacacc gactgccccc agtacgcgca catcggcaac catttcggca tcacgggctg
     1141 gtgccagttg ctcgcgcagc tgcacttcaa tagccgccac ctgctgctgc cattcgccgg
     1201 attcgataat cgccaggctg gcgtttgctg ccgcgcaggc cagcggattg cccataaaag
     1261 ttggcccatg cataaagcag ccggcttcgc cgttactgat ggtttctgca acctcgcgcg
     1321 tggtaagtgt ggcggaaagg gtcattgtgc cgccggttaa ggctttaccg aggcacaaaa
     1381 tgtccggcgc gatttctgca tattcacagg caaacagttt gccggtacga ccaaatccgg
     1441 tggcgatctc gtcggcaatc agcaagatac cttcgcgatc gcacattttg cggattcgtt
     1501 ttaaccattc cggatggtac atgcgcatcc cgcctgcgcc ctggacaatc ggctcaatga
     1561 tcaccgctgc gatttcatga cgatgtgccg ccatcaggcg ggcaaaacca accatatcgc
     1621 gttcatccca ttcaccatcc atgcggcttt gcggggcggg agcaaacaag ttttctggca
     1681 gatagccttt ccacaggctg tgcattgagt tgtccggatc gcacaccgac atcgcgccaa
     1741 aggtatcgcc atgataacca ttgcggaagg tcagaaaacg ctggcgcgct tcgcctttgg
     1801 cttgccagta ctgcaacgcc attttcatcg ccacttccac cgctacggaa ccggagtccg
     1861 cgagaaaaac gcactccagc ggttgcggcg tcattgccac cagtttgcgg catagctcga
     1921 tggcgggcgc atgggtgata ccgccaaaca tcacatgcga catggcatca atttgcgtct
     1981 tcatcgccgc attaagctgc gggtgattgt agccgtggat cgccgcccac caggacgaca
     2041 taccgtcaac caggcgtctg ccgtcagaca aaattagctc gcaaccttcg gcgctcgcca
     2101 ccggataaac cggcagaggg gaggtcatgg atgtgtatgg gtgccagata tggcgttggt
     2161 caaaggcaag atcgtccgtt gtcataatcg acttgtaaac caaattgaaa agatttaggt
     2221 ttacaagtct acaccgaatt aacaacaaaa aacacgtttt ggagaagccc catggctcac
     2281 cgcccacgct ggacattgtc gcaagtcaca gaattatttg aaaaaccgtt gctggatctg
     2341 ctgtttgaag cgcagcaggt gcatcgccag catttcgatc ctcgtcaggt gcaggtcagc
     2401 acgttgctgt cgattaagac cggagcttgt ccggaagatt gcaaatactg cccgcaaagc
     2461 tcgcgctaca aaaccgggct ggaagccgag cggttgatgg aagttgaaca ggtgctggag
     2521 tcggcgcgca aagcgaaagc ggcaggatcg acgcgcttct gtatgggcgc ggcgtggaag
     2581 aatccccacg aacgcgatat gccgtacctg gaacaaatgg tgcagggggt aaaagcgatg
     2641 gggctggagg cgtgtatgac gctgggcacg ttgagtgaat ctcaggcgca gcgcctcgcg
     2701 aacgccgggc tggattacta caaccacaac ctggacacct cgccggagtt ttacggcaat
     2761 atcatcacca cacgcactta tcaggaacgc ctcgatacgc tggaaaaagt gcgcgatgcc
     2821 gggatcaaag tctgctcagg tggcattgtg ggcttaggcg aaacggtaaa agatcgcgcc
     2881 ggattattgc tgcaactggc aaacctgccg acgccgccgg aaagcgtacc aatcaacatg
     2941 ctggtgaagg tgaaaggcac gccgcttgcc gataacgatg acgttgatgc ctttgatttt
     3001 attcgcacca ttgccgtagc gcggatcatg atgccgacct cttacgtgcg cctttctgcc
     3061 ggacgcgagc agatgaacga acagactcag gcgatgtgct ttatggcagg cgcaaactcg
     3121 attttctacg gttgcaaact gctgaccacg ccgaatccgg aagaagataa agacctgcaa
     3181 ctgttccgca aactggggct aaatccgcag caaactgccg tgctggcagg ggataacgaa
     3241 caacagcaac gtcttgaaca ggcgctgatg accccggaca ccgacgaata ttacaacgcg
     3301 gcagcattat gagctggcag gagaaaatca acgcggcgct cgatgcgcgg cgtgctgccg
     3361 atgccctgcg tcgccgttat ccggtggcgc aaggagccgg acgctggctg gtggcggatg
     3421 atcgccagta tctgaacttt tccagtaacg attatttagg tttaagccat catccgcaaa
     3481 ttatccgtgc ctggaagcag ggggcggagc aatttggcat cggtagcggc ggctccggtc
     3541 acgtcagcgg ttatagcgtg gtgcatcagg cgctggaaga agaactggcc gagtggcttg
     3601 gctattcgcg ggcgctgctg tttatctctg gttttgccgc gaatcaggca gtcattgccg
     3661 cgatgatggc gaaagaggac cgtattgttg ccgaccggct tagccatgcc tcattgctgg
     3721 aggctgccag tttaagcccg tcgcagcttc gccgttttac tcataacgat gtcgctcatc
     3781 tggcgcgact gcttgcttcc ccctgtccgg ggcagcaact ggtggtgaca gaaggcgtgt
     3841 tcagcatgga cggcgatagc gcgccactgg cggaaatcca gcaggtaacg caacagcaca
     3901 atggctggtt gatggtcgat gatgcccacg gcacgggcgt tatcggggag caggggcgcg
     3961 gcagctgctg gctgcaaaag gtaaaaccag aattgctggt agtgactttt ggcaaaggat
     4021 ttggcgtcag tggggctgcg gtgctttgct caagtacggt ggcggattat ctgctgcaat
     4081 tcgcccgcca ccttatctac agcaccagta tgccgcccgc tcaggcgcag gcattacgtg
     4141 cgtcgctggc ggtcattcgc cgtgatgagg gggatgcacg gcgcgaaaaa ctggtgtcac
     4201 tcattgcgcg ttttcgtgcc ggagtacagg atttgccgtt tacgctggct gattcatgca
     4261 gcgccatcca gccattgatc gtcggtgata acagccgtgc gttacaactg gcagaaaaac
     4321 tgcgccagca aggctgctgg gtcacggcga ttcgcccgcc aaccgtaccc gctggtactg
     4381 cgcgactgcg cttaacgcta accgctgcgc atgaaatgca ggatatcgac cgtctgctgg
     4441 aggtgctgca tggcaacggt taataaacaa gccattgcag cggcatttgg tcgggcagcc
     4501 gcacactatg agcaacatgc agatctacag cgccagagtg ctgacgcctt actggcaatg
     4561 cttccacagc gtaaatacac ccacgtactg gacgcgggtt gtggacctgg ct
//
LOCUS       DBKEKI010000222         4511 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.224, whole genome shotgun sequence.
ACCESSION   DBKEKI010000222 DBKEKI010000000
VERSION     DBKEKI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.224"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..63)
                     /locus_tag="ACUQPX_004128"
     CDS             complement(<1..63)
                     /locus_tag="ACUQPX_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HHV9058597.1"
                     /translation="MKKNQFLKESDVTAESVFFMK"
     gene            complement(172..585)
                     /gene="hiuH"
                     /locus_tag="ACUQPX_004129"
     CDS             complement(172..585)
                     /gene="hiuH"
                     /locus_tag="ACUQPX_004129"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HHV9058598.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            718..1389
                     /gene="hprR"
                     /locus_tag="ACUQPX_004130"
     CDS             718..1389
                     /gene="hprR"
                     /locus_tag="ACUQPX_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HHV9058599.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1389..2747
                     /gene="hprS"
                     /locus_tag="ACUQPX_004131"
     CDS             1389..2747
                     /gene="hprS"
                     /locus_tag="ACUQPX_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HHV9058600.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2855..3706)
                     /gene="hchA"
                     /locus_tag="ACUQPX_004132"
     CDS             complement(2855..3706)
                     /gene="hchA"
                     /locus_tag="ACUQPX_004132"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HHV9058601.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(4306..>4511)
                     /locus_tag="ACUQPX_004133"
     CDS             complement(4306..>4511)
                     /locus_tag="ACUQPX_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HHV9058602.1"
                     /translation="GDSGGQSTGYKDSDRLNYIEIGTWYYFNKNMNIYTAYQINLLDK
                     SDYVLAHGLNTDDQLAVGIVYQF"
BASE COUNT         1277 a          872 c         1048 g         1314 t
ORIGIN      
        1 cttcataaag aataccgact cggccgtaac atctgattct tttaaaaatt gattcttttt
       61 cataagccat cacacttttt tgtcgttaac caacaagagc tgtttactgt taatctgtca
      121 gggagtcacc tccctaacag tagcagagat cagcttttac atatttacgc attaactgcc
      181 acgataggtt gaatacccat attggctgag taacaaaggc acatgataat gctcgttcac
      241 tttattaata tgaaattcaa cgggaatctc agggaagaaa ctttcaagat tttgtttctt
      301 gaaatagtcc ccggttttaa atacgacacg gtaatcgcca gtagtcgcag tttgctcggg
      361 ccacagtgcc ttaattcgtc catccttatc tgttttggcg gtattaagtt gtaaccagcc
      421 gttatccgct ttcttttcaa gagtgactgt cacgtcggca gcgggttttc ctgtttgctg
      481 gttcaaaatg tgcacgctaa gaatgttttg ttgtgcggca taaaccaaag aaggtaatga
      541 aaatgctgcc gttgctaccg agagtactaa ataacgcttt aacatgttta tatccttgtc
      601 atgtgaatga gttttgagga tagcgaaaaa acgtccaggg taaccttaca gcagcattac
      661 aattttgtaa tgaatcaggt tgtttctata acatatgatt tatggcatat tattttcatg
      721 aagattctac ttattgaaga taatcaaagg acccaggaat gggtaacgca ggggctttcc
      781 gaagcgggtt atgtcattga tgccgtttct gatggcagag atgggcttta tcttgcgctg
      841 aaggatgatt atgcattgat cattctggat attatgcttc cgggtatgga tggctggcag
      901 atcttacaaa cgttaagaac ggcaaagcaa acccctgtta tttgccttac tgcaagggat
      961 tctgtcgatg acagagtcag agggttggac agtggggcaa atgattatct ggtaaaacct
     1021 ttttcatttt ctgagttgct agcaagggtt cgggcacaat taaggcaaca tcacgctctg
     1081 aattcaacat tagaaatcag tggcttaaaa atggactctg tcagtcaaag tgtgagcagg
     1141 gacaatatca gtattacact gacgcgcaag gagtttcagt tactttggct actggcctcc
     1201 agagctggcg agattatacc cagaacggtt attgcgagtg aaatttgggg aatcaacttt
     1261 gatagtgata ccaatacggt ggatgtcgcc attcgcaggc tccgcgcaaa agttgatgat
     1321 ccttttcctg aaaagctaat cgccacaatc cgggggatgg gctattcatt cgtagcggta
     1381 aaaaaataat gaaaagatta tctataaccg tccgtttaac cttgcttttt atatttctgc
     1441 agtctgttgc tggcgctgga attgtatgga ctctctataa tggcctggca agtgagttga
     1501 aatggcgcga tgatacaaca ctcattaacc ggacagcgca gatcaagcag ttgttaattg
     1561 atggggtaaa tccagatacg ttacctgtgt actttaaccg gatgatggat gttagtcagg
     1621 atattttgat cattcatagt gatggcatca ataaaattgt taaccggaca aatgtcagtg
     1681 atgacatgtt aaataacata cctgctagtg agacaatcag cgcagcaggc atttacagaa
     1741 gcattattaa tgatacagag atagatgctt tacgaattaa cattgatgaa gtttcgccat
     1801 cattaacggt tactgtggct aaattggctt cagccagaca taatatgctt gaacagtata
     1861 aaattaatag cattataatt tgcattgtcg ccattatact gtgctcagta ttaagtccgt
     1921 tgttaatcag gacggggttg cgagagatca aaaagttgag tggcgtaacg gaagcgctga
     1981 attataacga tagccgagtg ccggttgagg ttaacgcatt accgagagaa ctaaaacctc
     2041 ttgggcaggc gttgaataaa atgcatcatg ctttagtcaa agattttgag cgccttagtc
     2101 aatttgctga cgatctcgct catgaactta gaacgccaat taatgcatta ctgggtcaga
     2161 atcaggttac gctcagtcaa accagaagta tcgctgaata tcaaaaaaca attgccggaa
     2221 acattgaaga gctggaaaat atttcgcggt taacagagaa tatactgttt cttgccaggg
     2281 cagacaaaaa caatgttttg gtgaaactgg attcgctttc tctcaataag gaagttgaaa
     2341 atcttttgga ctatcttgaa tatctttcag acgagaaaga gatttgcttt aaggtcaagt
     2401 gcaatcaaca aatctttgcg gataaaattt tactgcaacg aatgttatcg aatcttattg
     2461 ttaacgccat tcgatattca ccagaaaaat cgcgtattca tataaccagt tttcttgatg
     2521 ccaacggctc tcttaatatt gatatcgcca gtcctggaac gaaaattaat gagcctgaaa
     2581 aactcttccg tagattttgg cggggagata attcgcgtca ttccgtaggt cagggacttg
     2641 gcctttcttt agtcaaagcg attgccgaat tacatggagg aagtgctacg tatcactatc
     2701 tcagtaagca taacgtgttc cgaattacgt taccgcaaag aaattaacat cgggcttcag
     2761 tacgccataa gttaaccgtg aaaaaacgca gccacgtcgt gagtattaac gtggctgcga
     2821 ttgattatgc gcttacatca caatgtaaca gggattaact cgcgtaagct gccagcattt
     2881 cctgcgccgc cagtttaccc aacgcattcg ctgcaaaagg actgtcgccg gtgagaagtt
     2941 tacggtcctt atgtactcgc ccggtgatgt cgtcattaat gatgttcatg cccattttct
     3001 tcaactcttc gccgaagtac caggtgagat gacccggcat atagccaata tctggcgttt
     3061 gtttgtctgc ggcgtctggg aatgcgcaaa tagaataacc atttagtggg ttatcgctgt
     3121 ggcgaagtgc cagaaaagcc gccggaccgt ggcaaaggga gataacaaaa cggtcatttt
     3181 tgattgccca ctgtaaagcg gcagccacgt cctggctttc tggtaaacca ataagtgcgc
     3241 catgcccacc aggaacaaag attgctgcat attcgctatc agcgttgagg ctggcgacaa
     3301 catccgcgag tttcttcgga ttacggaata acgatttatg ctgttcaaag aatggcatta
     3361 ctttttcatc tttgtgcggc atagcccagt attcaaactt ggtcatcaga ccggaaatgg
     3421 tcgccacttc gaattcgaaa cctgcagcat ggagatgata caacggcagc aacgtttcaa
     3481 tcggatggtt accggtcgag aacagttttc cgttatcggt cggcaaataa cgttcgtccg
     3541 cggcgatcac cagaatttta tgtttaccgc gatacggttt tggatagtcc acgccatcaa
     3601 gatcagagac gggactcgta tattggctaa gcgaatattc tgaagggaag aacgcattat
     3661 cttcagcaat atcgacctgc gggtttttac ttgtttgaac agtcatagtg tattccttat
     3721 tgttgcttag ttagggtaat cactatattt gcgactgagc tggtcgctat ttttaaccag
     3781 gctgaaaaat tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat
     3841 gatgctgcac tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc
     3901 gcaagcgcaa gtggcctcaa tgattttaga ccattatcag aggtccggag cggcgggaag
     3961 agatttattg ctgtggaata attgtggtac tgatgattat ttatatcccg gattggtagg
     4021 gaaaagcttt tttcatgcac tatttttgca aatatttcta tatgggaagc ggttttgtta
     4081 cagattaaat ttataaagaa atgcatttgg ggtgttgggt tgaagagtag ctggtagatg
     4141 ttattgtgcc agcaggggca ggggaatgtg ccgtatcgat atgctttggc aattgcgtgt
     4201 gccgcagatg gagatgaaat gccaataaaa aagcctgagt tcgatgaggg acgaactcag
     4261 gctggtgtta tgttttacag taacacgcct cggcgcgttg gctgattaga actggtagac
     4321 gataccaact gccagttggt cgtcggtgtt cagtccgtga gcgagaacat aatcactctt
     4381 atcaagcagg ttgatctgat aagcggtata tatgttcata ttcttattga agtaatacca
     4441 ggtaccgatt tcaatatagt tcagacgatc agaatctttg taacctgtag actggccccc
     4501 tgaatctcca g
//
LOCUS       DBKEKI010000223         4377 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.225, whole genome shotgun sequence.
ACCESSION   DBKEKI010000223 DBKEKI010000000
VERSION     DBKEKI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.225"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1064
                     /gene="aldA"
                     /locus_tag="ACUQPX_004134"
     CDS             <1..1064
                     /gene="aldA"
                     /locus_tag="ACUQPX_004134"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HHV9058603.1"
                     /translation="IQSDRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTG
                     NTIVIKPSEFTPNNTIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMT
                     GSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCN
                     CAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAALERVEQKVARA
                     VEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAIS
                     MANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHAGWRKSGIGGA
                     DGKHGLHEYLQTQVVYLQS"
     gene            complement(1106..2107)
                     /gene="gap"
                     /locus_tag="ACUQPX_004135"
                     /pseudo
     CDS             complement(1106..2107)
                     /gene="gap"
                     /locus_tag="ACUQPX_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            2296..2826
                     /gene="cybB"
                     /locus_tag="ACUQPX_004136"
     CDS             2296..2826
                     /gene="cybB"
                     /locus_tag="ACUQPX_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HHV9058604.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3071..3244
                     /locus_tag="ACUQPX_004137"
     CDS             3071..3244
                     /locus_tag="ACUQPX_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058605.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            3270..3338
                     /gene="yncP"
                     /locus_tag="ACUQPX_004138"
     CDS             3270..3338
                     /gene="yncP"
                     /locus_tag="ACUQPX_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HHV9058606.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(3316..3465)
                     /gene="hokB"
                     /locus_tag="ACUQPX_004139"
     CDS             complement(3316..3465)
                     /gene="hokB"
                     /locus_tag="ACUQPX_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HHV9058607.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(3565..4377)
                     /locus_tag="ACUQPX_004140"
                     /pseudo
     CDS             complement(3565..4377)
                     /locus_tag="ACUQPX_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT         1123 a          995 c         1057 g         1202 t
ORIGIN      
        1 ttattcaaag cgatcgtcca ggagaaaata ttcttctgtt taaacgtgcg cttggtgtga
       61 ctaccggcat tctgccgtgg aactttccgt tcttcctcat tgcccgcaaa atggctcccg
      121 ctcttttgac cggtaatacc atcgtcatta aacccagtga atttacgcca aacaatacga
      181 ttgcattcgc caaaatcgtc gatgaaatag gccttccgcg cggcgtgttt aaccttgtac
      241 tggggcgtgg tgaaaccgtt gggcaagaac tggcgggtaa cccaaaggtc gcaatggtca
      301 gtatgacagg cagcgtctct gcaggtgaga agatcatggc gactgcggcg aaaaacatca
      361 ccaaagtgtg tctggaatta ggtggtaaag caccggctat cgtaatggac gatgccgatc
      421 ttgaactggc agtcaaagcc atcgttgatt cacgcgtcat taatagtggg caagtgtgta
      481 actgtgcaga acgtgtttat gtacagaaag gcatttatga tcagttcgtc aatcggctgg
      541 gtgaagcgtt gcaggcggtt caatttggta accccgctga acgcaacgac attgcgatgg
      601 ggccgttgat taacgccgcg gcgctggaaa gggttgagca aaaagtggcg cgcgcagtag
      661 aagaaggggc gagagtggcg ttgggtggca aagcggtaga ggggaaagga tattattatc
      721 cgccgacatt gctgctggat gttcgccagg aaatgtcgat tatgcatgag gaaacctttg
      781 gcccggtgct gccagttgtc gcatttgaca cgctggaaga tgctatctca atggctaatg
      841 acagtgatta cggcctgacc tcatcaatct atacccaaaa tctgaacgtc gcgatgaaag
      901 ccattaaagg gctgaagttt ggtgaaactt acatcaaccg tgaaaacttc gaagctatgc
      961 aaggtttcca cgccggatgg cgtaaatccg gtattggcgg cgcagatggt aaacatggcc
     1021 tgcatgaata tctgcagacc caggtggttt atttacagtc ttaatgagtg aaagaggcgg
     1081 aggttttttc ctccgcctgt gcgcgtcaga gtttagcgaa tttttcgagg gtgcgaataa
     1141 gctgcgtgac gaagccatat tcgttatcgt atcaggcgac cgttttcacc agttgtaaat
     1201 cgcccacggc ggtaatttcc gtttgcgtgg catcaaacac tgaaccgaaa tggctgccaa
     1261 tgatatcgga agagactatt tcttcatcgg tataaccaaa tgactcgtta ttggtggttg
     1321 cttgtttaag tgcgttattc acctcttcgg cagtcacttt ttttccgaga atggacacca
     1381 gctcagtgac cgaacctgtt ttcaccggca cgcgttgcgc atgacctttc agtttgccgc
     1441 tcagttccgg gatcaccaga ccaatggctt ttgccgcccc cgtagtgtgg ggaatgatat
     1501 tttctgccgc tgcgcgtgaa gcacgtaaat ctttaccacg tgggccatcc accagtgact
     1561 gggtgccagt ataggcatga atggtcgtca tcgtgccgac ttctatcccg aaactgtcat
     1621 gcaaggcttt ggccatcggc gcaaggcagt tagtggtgca tgacgccacg gaaacaatgg
     1681 tgtcgttgcc atccagagtg tcgtcattaa cgttataaac gatggttttc atttcaccgg
     1741 caggagcgga agtcaacacc ttcttcgcac cagcatcaag atgcgcctgc gatttatcgg
     1801 cggaggtata aaagccagta cattcgacaa tgatttctgc ccctttcgct ttccacggaa
     1861 tatttttagc ctctttttcg gcgtaaaccg cgatactttt cccatcaacg ataagtgaat
     1921 cttccgtaaa atcaacgctc caggggaacg gaccatagtt tgaatcatgt ttcagcagat
     1981 aggcgagaat ttttggggaa gtaagatcat taatagcgac aacgtctatg ttgcttttga
     2041 cttcaagtaa acgacgcaac accagtcgac cgatacgacc aaaaccgtta ataccaactt
     2101 tactcatggt tttctcctgt caggaacgtt cggatgaaaa ttgatccttt ccaggcttag
     2161 accaggattg tgggatgggc aatctccatt cttacagtga aacgtaacgt actgaaaacg
     2221 ggtgaacaat atttaatgaa attttgagaa aagcccgtta tgttaacgga aaattatgtt
     2281 aaagcaggaa atgttatgga aaataaatac tcaaggttac aaatcagcat tcactggctg
     2341 gtctttttgc tggttatcgc agcgtattgc gcaatggagt ttcgtggttt cttcccacgt
     2401 agcgatcggc cgctcatcaa catgattcat gtttcctgtg gcatctcaat tctcgtgctg
     2461 atggtggttc gtcttttgtt aagactgaaa tacccaaccc cgccgattat acctaagccg
     2521 aaaccgatga tgacgggact ggcgcatttg ggacatttgg tgatttatct cctgtttatt
     2581 gcgctgccag tgattggttt ggtgatgatg tataaccggg gcaacccgtg gtttgcgttt
     2641 ggtttgacga tgccttacgc ttcagaggcc aatttcgaac gggttgatag cttaaagtcg
     2701 tggcatgaga cgctggcgaa tctgggatat tttgtcatcg ggttgcacgc tgcggctgca
     2761 ctggcacacc actatttctg gaaggacaac acacttctac gcatgatgcc gcgtaaacgt
     2821 tcctgaagga tatttaaaga aaacgcctgt actaaaaccg acccgtggta caggcgaaga
     2881 atacgggtct acatcggaag cgcctattat atttatttgt atgacaaata aaaccataat
     2941 ccttgcccat acgtccatct ggcttatttt taatcaattc acccgatctt tgatctcatc
     3001 aacggtatca aaataaacca gcgtataacg ttcaaatcgc acataagatt cgataaacca
     3061 tggaggttat atgaaaaaac tggcacttat tttgtttatg ggaacgcttg tttcctttta
     3121 tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg gggatctcac actgtacggc
     3181 aggcggcaaa tttgtctgta atgatggttc tattagtgca tcgaaaaaaa catgcactaa
     3241 ctgaagtgta aaaggggtgc catgagaaaa tgaatctgct agtcagatgc gcggggaaaa
     3301 tccccgcgct tgcccttacc tggacgtgca ggccatgagc gcagcaacct ccttatcacc
     3361 gtcccggaac cgcagttcgt agagcgtttg tcgggtcagg agtgtgaatg ccagaatcgt
     3421 aatgcagata atgagcagac acaccaccag agggttgcgc ttcatagcct ttctccttgc
     3481 cggatggcgg gtaagaggct aacatctgaa ttgcttggtt cattcgttgg cctcggtttg
     3541 tactgacccc aaaaagttgg acagttaaac acgaggcata taggtctgat tccgatattc
     3601 atttggagtc agacctttta atttcaggct aattcttctg ctgttgtagt attcaatata
     3661 ttccgtaaca gcatccttca gttcgcttat attactgaac tcatcaagat aaaaacactc
     3721 cgactttaag gttccaaaga aacactccac cacagcatta tccagacaat tgccttttct
     3781 ggacatgctt tgtttaatac catgttcttt aaggatattt tgatatcttc tcatacgata
     3841 ctgccatccc tggtcagagt gcagaacagg atgctcgtga ggattaagca tttttgaatg
     3901 cctgatcgag catattctca accatgttca tcactggtct ttccgaaagg ctgtaagaaa
     3961 taacttcgtt gttgaagaga tctattactg gagacaaata cagcttgcgc ccattgactg
     4021 caaattcagt aacatcggta acccacttct cgtttgcccg cgtagccttg aaatctcttt
     4081 ggagaacatt aggggcggtt tgccctacct ctcctctgta agagcggtat cgcttgacct
     4141 taatcgctgc tttaagtgag agggttccca tcaggcgctg aacagcttta tggttaatct
     4201 gtttcccttc tcgatgaaga gacagcgtta ccctacggta tccgtatcgg cctctattct
     4261 cgtgataaat ctcaccaata cgctttttaa cgtccgcata cttgtcaggc ttgctgagag
     4321 cctttagatg gtaataaaac gtactgcgcg gtatctccgc agccctgaga agctcat
//
LOCUS       DBKEKI010000224         4376 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.226, whole genome shotgun sequence.
ACCESSION   DBKEKI010000224 DBKEKI010000000
VERSION     DBKEKI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.226"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1232)
                     /gene="hglS"
                     /locus_tag="ACUQPX_004141"
                     /gene_synonym="ydcJ"
     CDS             complement(<1..1232)
                     /gene="hglS"
                     /locus_tag="ACUQPX_004141"
                     /gene_synonym="ydcJ"
                     /EC_number="1.13.11.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoadipate dioxygenase/decarboxylase HglS"
                     /protein_id="HHV9058608.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEAPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHG"
     gene            1449..2372
                     /locus_tag="ACUQPX_004142"
     CDS             1449..2372
                     /locus_tag="ACUQPX_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HHV9058609.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(2410..4050)
                     /gene="trg"
                     /locus_tag="ACUQPX_004143"
     CDS             complement(2410..4050)
                     /gene="trg"
                     /locus_tag="ACUQPX_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HHV9058610.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SKQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRNRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
BASE COUNT         1048 a         1153 c         1036 g         1139 t
ORIGIN      
        1 ccgtgactgc gtgtctgcgt ttcattaccc agatttgact ggaagatccc cgccgcgcta
       61 acgggcaaga aatcttcata ggtgatgggt tgcgccacta cccaaccacg ttcaattaag
      121 ggctgtggat cgtctccagg atgaatcgcc tgacgatgcg cctcacccga aggcgtcaga
      181 cggtaccgga accatgccaa cccttgctga cgcattaaaa actcactgtc aggaaaagtg
      241 cggaaggttt cctgtaaatg catttggtga gtgagattat cctgcccggt tccagcgtta
      301 cgcagaagat catcatacag ttgtcgccct ttcggcgtta atgccacgcc acgctgctca
      361 atttcaccaa agcgcgcggt atgcgtgccc tgtttctgcc ccgcaaacaa caccgtctct
      421 tccagtgctt taaagctggt ctggcgtagt aaaatcggta cctcgcggcg cggcggcccc
      481 tcgatgagaa ttttgggttc aattccgcat tcaggcatca tcgactgcac ccggtcaata
      541 tccagcgtac gtggcgtcag gtggttgata tggcatccag gaaaacagac cacatcagca
      601 atcaaccgat gttcgttgtg caaggcgcga taggtttctt catctaccgt tgctaactgg
      661 tgccagcgaa acgtttccag ggcttcctgc acaaactcct gtgcctgtgt ttcgttaaaa
      721 ccgccctgct gctcatattc ctctaacagt tgtcgacaac gtggggtgaa gatatcgcgc
      781 tgacgcagaa tctccgccgc tttctggcgc aaaatttcgt tctcgataag ctcaaggcgg
      841 agtaaggatg taaaaacgcg gaagggatta cgcgccagag aagcatcatc aatgggccga
      901 aatgctgtcg aatgtaccgg cacccctgcc tgcgagagat cgtaatagct caccgggtac
      961 atccccataa tggcaaacat ccgccgaaga gtagcaagct cttgtgcagt cccaacgcga
     1021 atcgccccat gacgttcaac attcagtcgc gccagctcgt ctgcatttac cattttttcg
     1081 tgcagttgag gattgttttc cagcacagcc agattcacat cagctaccag ttccagcagc
     1141 gtgccatatt gcggagcttc ttgctggtac atggctgaca ttgcctgcga aaactgttcc
     1201 cgaatctcat ccgccgtgat gctgttcgcc atagtgtctg cctccagtgg tcagtaatct
     1261 ggagtgtagg taaccgcatt cactctggcg ggaagaattt acaaactgtg atctcgccgc
     1321 gaaaacatca atattatcca ttttgctgta acataattgc tttaattgtt aataatattt
     1381 tgcaatcaag ttatcataat caaacaactt cacttgtcag cgacaccgct tcgtttttaa
     1441 catcgcttat ggaaaaaaat agtctgttta gtcagcgcat ccgtttgcgc caccttcata
     1501 cattcgtagc tgtcgcacaa caaggaactt tggggcgcgc ggctgaaacc cttaatttga
     1561 gtcaacctgc gctctctaat acattgaatg aactggagca gctgacgggc gctcgcttgt
     1621 ttgagcgtgg tcgtcagggg gcgcaactta ccttacccgg cgaacaattt ttaacgcatg
     1681 cagtcagagt tcttgacgcc atcaacactg ccggacagtc gcttcatcgt aaagaaggtc
     1741 ttaataatga tgtcgtcagg gttggtgcac tacctactgc ggcactgggg atattacctt
     1801 cggttatagg tcagtttcat cagcaacaaa aagagacgac cttgcaagtt gcgacaatga
     1861 gtaaccctat gattctggcg ggtttaaaaa ccggggaaat cgatatcggc attggtcgga
     1921 tgtcagatcc tgaactgatg accgggctta attacgaact gctgtttctt gaatcgttga
     1981 agctggttgt ccgccctaat cacccgctac ttcaggagaa cgtaacgcta agccgggtgc
     2041 tggaatggcc ggtcgttgta tcaccagaag gcactgcgcc acgccagcat tcagatgcat
     2101 tagtgcagag ccagggctgt aaaattcctt cgggttgtat cgaaacgctg tctgcttcgc
     2161 tatctcgtca acttaccgtt gaatacgatt acgtgtggtt tgtcccttct ggcgctgtaa
     2221 aagacgacct gcgtcatgcc acgctggtgg ccctgcctgt tccgggacat ggtgcaggcg
     2281 aaccgattgg aatactgacc cgcgtagatg cgacgttctc ttctggttgc cagttgatga
     2341 ttaacgctat tcgaaaatca atgccgttct gaaaggtgaa gggatctgtc gatccctcct
     2401 tgaacatttt cacaccgtag cgaaactaac tggttcaccc gctccgcgag gttctgccga
     2461 cacagaatgt ttgttcagac ggaatacgtt caccgcctca gttaatcgtg cagcctgttc
     2521 ttcaagggac accgccgctg ctgaggcctc ttctaccaga gaagcattct gttgcgtcac
     2581 cttatccatt tcagaaatcg cctggctaac ctgagttatg cctctacttt gttcatccga
     2641 ggcggcggcg atttcctgca tgatatgtgt gacactcgcg acggcatcaa caatagtgct
     2701 catggttttc cctgccgttg ccacctcatc cgaccccagg tcaattaacc tgactgattc
     2761 actgatcaag ccttcaatct ctttcgctgc ctgagcgctg cggcttgcga gtgtccgtac
     2821 ttcgctggcg acaacggcaa atccacgccc ttgctcaccc gctcgcgcgg cttcaacggc
     2881 agcattcagt gccagaatat tcgtctggaa agcaatactg ttgatgacgg cggtgatctc
     2941 agaaattttc ttcgaactcg tggagatagc gcccatcgtt tttactacac cggaaaccgt
     3001 ctgcccgcca tcgctggctt taatagaagc ctcttgcgcc agtttgctgg catgatgcgc
     3061 gttatccgca ttctgtttca ccgtcgcagt gagttgctcc atgctggcgg cagtttgttc
     3121 gatagccgcc gcttgttctt cggtgcgaga tgacagatcc gcattgccag ctgaaatttc
     3181 gctggtgcca cgataaatct cttccgcgcc ctgtcgaaca gtccctactg tcatccccag
     3241 tgaatgctgc atttgctgta aatgacgact taagcgaccg atttcattac gacccgccgg
     3301 ttcatcattc atcgtcagat cgccactggc gattttttca atccgttgtg cggcatgttg
     3361 cagtggacga atgacgatcc gacgtagcac cataaatgtt atcagcgtca tgaccagggc
     3421 aagcacaaac gcgccaatca tgaacatccc acccaggcgg ttgcgctgat gggccagttc
     3481 cgccagttgg ttggctctgg tgctacgtat cttaacggct ttgttcaaaa tatcggtata
     3541 agcattatcc agcggtcgga tctgcttact ttcatgattg ataatcgctt caaacatgcc
     3601 atttttggca tatttcaaca taggttgcat acccgtgata taagcctgaa agcgttgatt
     3661 taattcagtg tcgagggctt catcagcagg tgttttcacc ggtcgattct gataagcacg
     3721 ataaccttgc tgcgactgtt taatctccga ttcggcttgc gcaatatttc gcttcattgc
     3781 ttccatttcc gcaatacgac tcgcagcccc ggcctgaatc atattgatcc gggctgaacg
     3841 caaaaaatct gaactgttcg ctaacccggt ccgaatctca atctccgcag taacatcacg
     3901 ctgatcgcga tcggcctgcc agaggaaata gccagccaga gcagaactta atgcgaatag
     3961 aactaagata ccgccaagaa tgcaggcaaa taacggaacc agcctgatgt gatgcaaaaa
     4021 acctaatcgc tgtgagggag ttgtattcat taccgttgtc tctcgtccag gtttactcct
     4081 gatagaaagt catcggcact tgacggtaat tacttatgac caaaaacctc tttgcgtcac
     4141 atttttacaa caattattgc agagaaaatt aatgctattg gaatgaagag agtaacgaat
     4201 ggctaaactg gctaacccga atgacgaaaa attctggaaa acagcacgca atttcgtgaa
     4261 gtgattattt tcgtccctaa ggaaacctca taggatgcct gcgggtgcct gagactttct
     4321 gttttcaggc taaccgggaa tcagaaagac gaaggccccg accgatattt ctatca
//
LOCUS       DBKEKI010000225         4358 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.227, whole genome shotgun sequence.
ACCESSION   DBKEKI010000225 DBKEKI010000000
VERSION     DBKEKI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.227"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1103)
                     /locus_tag="ACUQPX_004144"
     CDS             complement(<1..1103)
                     /locus_tag="ACUQPX_004144"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - gamma-aminobutyrate shunt
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HHV9058611.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMELSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
     gene            complement(1314..2711)
                     /gene="ccp"
                     /locus_tag="ACUQPX_004145"
     CDS             complement(1314..2711)
                     /gene="ccp"
                     /locus_tag="ACUQPX_004145"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HHV9058612.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            3116..>4358
                     /gene="treF"
                     /locus_tag="ACUQPX_004146"
     CDS             3116..>4358
                     /gene="treF"
                     /locus_tag="ACUQPX_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HHV9058613.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPAFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALF"
BASE COUNT         1108 a         1137 c         1025 g         1088 t
ORIGIN      
        1 gtacagatga actcatacgg cccctgtttg gcgatttcat ccgccagata agcggcaacc
       61 tggtaagagg cgttctgtac tttggtatag ccttcacgac cgaggcgcag gaattcatag
      121 tactgtgcaa ttacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatttga
      181 ccacccaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccaggaag
      361 ccaccgctgg cagcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagcgcat cgtgcagcgg ttgtgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt gttttcgtca caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgtac cggaccgcac actaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatatt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt attgatcgac aactccatca atttatggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggctaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctggaatgc gacatcatcg cgcatttcgt gcagcggaaa tcgttttgac
     1021 tccgcgatag tagaaatggc ctttgcgcca aaacgtgaat cgagtagttc tgagcggaaa
     1081 tccgttaaca gcttctggtc catttcgaac tccttaaatt tatttgaagg caataaaaaa
     1141 gtaggattta tccgcaatgg aagcaaggca ttacaggctt aatttaaata acaaaatcct
     1201 aagcagaaaa acgacttttt ataatataaa catttaacat gataatatta aaaacagact
     1261 ttattatcgt aataaaaatc gcccaaacat tcctgttcgg gcgatcaaat taattattgt
     1321 ttatcctgca tatacggcgt gtacaccccg ttcagactgt gcaggaaagc tacgatatca
     1381 tccacatcct cctgcggcag ctctttgcct acctgatagc gcagcatcag tttcaccgcc
     1441 ccgtccagcg tcggcacgtc accgcgatgg aagtacggtg ccgttaaagc aacgttacgt
     1501 aaaccgggta ctttctgacg caatttatca cgctcttctt tagtcacatt catacgacca
     1561 atatccgccg ccgtaatttc cccaaagtta aagtcttttt tcagccccaa cggttcaaag
     1621 gaacgtccgc cgagaataat accaccatga caagttgcac atttattatc tttaaataat
     1681 tgatagcctt ttttctgttg cgccgtcagc gcattttcat ctccacgcaa ccatttatca
     1741 aatggggaat ccggcgtaat taatgttttc tcaaattcag caatggcatc agtaatattt
     1801 tcgccactga aaccttgcgg ataaacttcg aggaactgcg ctttaagctg cggatctttt
     1861 tccagcttag caataatttc gtcccaggat ttcgacgcca tttcaatcgg gttcaacggc
     1921 ggtccacctg cctgatcctg caatgttgcc gcacgaccat cccagaactg ctcaacgtta
     1981 aatactgagt taaataccgt cggcgcatta atcggcccaa ctgcgccacc aacaccaatc
     2041 gatgtttttc tgccatcgac gccccccgca ttcaacgcat ggcaatgagc gcatgaaatg
     2101 gtgctatcag ccgataaacg gggatcgtga tacagcgcaa agcccaacgc cactttttgc
     2161 gcatcggtag gcagtttttg cgggatgggc tgcaccggtt cattgcgatg ctccggagca
     2221 gtatcattgc tggcgtaata ttccgcgcgc tgttttgcaa tccaggccag tatttccgcc
     2281 cgctcttcat cactcacctt acccgcccag tgtagcgcgg tataacgcgt tggtggcata
     2341 gtttcatact gcatcaccca ttcaatctta ttcagatcgc tttgcgaaac gggtttatca
     2401 gccagcagtg ccgcacgcac ggcctcgagg ttaaaagatt tatatccaag cttaatgtcg
     2461 taatccatca actgtttcgc gccaggaata taataatagg cgggtaattc tgccgaaggc
     2521 gtgtggcaat agtcgcatcc tttttcgcgg agaaagccta aaaccttatt attttcactg
     2581 acagcagatg cctgaacatc ggcctgttta ctgcgtttat tatcgtggta ccacacataa
     2641 ccagataacc ctaaatagca aatcgcgacg ccagccaggc cgatcgcggt aatacgtgag
     2701 accattttca ttattcttcc ctcacggtgg tgagttattg tgacccagaa aattttccag
     2761 aatgctgcaa acgtctctgc gagacaaagc catcataaaa aacagggaga tacgcttttg
     2821 gataggaatc aatcaattac atgaatataa actatcaata agatagcctg tgcctattac
     2881 ccagatcagg aatggcatat catgattgcg gcgagagcag gattgaatgt tctgaaaatg
     2941 aaaaacaaca ccaaatccgt atttttataa attccattga tattagtgcg ttatattttt
     3001 tgacaagtta ataaacgtga tccaccgcac gctttgtcgc ccaccaggcg gagcgaatga
     3061 ctacccttaa agaaaagccc gataattagc gacgaatttc ggaggttgga tccttatgct
     3121 caatcagaaa attcaaaacc ctaatccaga cgaactgatg atcgaggtcg atctctgcta
     3181 tgaactggac ccgtatgaat taaaactgga tgagatgatc gaggcagaac cggaacccga
     3241 gatgattgaa gggctgcccg cctctgatgc gctgacgcct gccgatcgct atctcgaact
     3301 gttcgagcat gttcagtcgg cgaaaatttt ccccgacagt aaaacctttc ccgactgcgc
     3361 acccaaaatg gacccgctgg atattttaat ccgctaccgt aaagtgcgcc gtcatcgtga
     3421 ttttgacttg cgcaagtttg ttgaaaatca cttctggctg ccggaggtct actccagcga
     3481 gtatgtatcg gacccgcaaa attccctgaa agagcatatc gaccagctgt ggccggtgct
     3541 aacccgcgaa ccacaggatc acattccgtg gtcttctctg ctggcgctac cgcagtcata
     3601 tattgtcccg ggcggccgtt ttagcgaaac ctactattgg gattcctatt tcaccatgct
     3661 gggactggcg gaaagtggtc gggaagattt gctgaaatgc atggccgata acttcgcctg
     3721 gatgatcgaa aactacggtc acatccccaa cggcaaccgc acctattatt tgagccgctc
     3781 gcaaccaccg gcttttgcgc tgatggtgga gttgtttgaa gaagatggtg tacgcggtgc
     3841 gcgccgctat ctcgaccacc ttaaaatgga atatgccttc tggatggacg gtgcagaatc
     3901 gttgatccct aatcaggcct atcgccatgt tgtgcggatg ccggacggat cgctgctcaa
     3961 ccgttactgg gacgatcgcg acacgccgcg tgacgaatcc tggcttgagg acgttgaaac
     4021 cgcgaaacat tctggtcgcc cgcccaacga ggtgtaccgc gatttacgcg cgggagcggc
     4081 ctccggttgg gattactctt cccgttggct gcgtgatact ggtcgtctgg cgagcattcg
     4141 taccacccag ttcatcccca tcgatctgaa tgccttcctg tttaaactgg agagcgccat
     4201 cgccaacatc tcggcgctga aaggcgagaa agagacagaa gcgctgttcc gccagaaggc
     4261 cagtgcccgt cgcgatgcgg taaaccgtta cctctgggat gatgaaaacg gcatctaccg
     4321 cgattacgac tggcgacgcg aacaactggc gctgtttt
//
LOCUS       DBKEKI010000226         4183 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.228, whole genome shotgun sequence.
ACCESSION   DBKEKI010000226 DBKEKI010000000
VERSION     DBKEKI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.228"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..209)
                     /locus_tag="ACUQPX_004147"
     CDS             complement(<1..209)
                     /locus_tag="ACUQPX_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HHV9058614.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACUQPX_004148"
     tRNA            complement(324..399)
                     /locus_tag="ACUQPX_004148"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACUQPX_004149"
     tRNA            complement(406..480)
                     /locus_tag="ACUQPX_004149"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACUQPX_004150"
     tRNA            complement(597..681)
                     /locus_tag="ACUQPX_004150"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACUQPX_004151"
     tRNA            complement(690..765)
                     /locus_tag="ACUQPX_004151"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACUQPX_004152"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACUQPX_004152"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HHV9058615.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACUQPX_004153"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACUQPX_004153"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HHV9058616.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACUQPX_004154"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACUQPX_004154"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HHV9058617.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAVVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1079 a         1040 c          932 g         1132 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaac ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tag
//
LOCUS       DBKEKI010000227         4168 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.229, whole genome shotgun sequence.
ACCESSION   DBKEKI010000227 DBKEKI010000000
VERSION     DBKEKI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.229"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            244..1344
                     /gene="ompC"
                     /locus_tag="ACUQPX_004155"
     CDS             244..1344
                     /gene="ompC"
                     /locus_tag="ACUQPX_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HHV9058618.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1508..1993
                     /locus_tag="ACUQPX_004156"
     CDS             1508..1993
                     /locus_tag="ACUQPX_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HHV9058619.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            2103..3434
                     /locus_tag="ACUQPX_004157"
     CDS             2103..3434
                     /locus_tag="ACUQPX_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HHV9058620.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYTPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            3758..>4168
                     /gene="narU"
                     /locus_tag="ACUQPX_004158"
     CDS             3758..>4168
                     /gene="narU"
                     /locus_tag="ACUQPX_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HHV9058621.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTINLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALL"
BASE COUNT         1341 a          840 c          708 g         1279 t
ORIGIN      
        1 tacggcccgg cagttaatca gataaattcc gacaatatta aaaaataaat ttcaaatacc
       61 agtaattcac cttatatctc atttcgactt cgatcacata tcgataaata atattatttc
      121 atctattttt ctcattgttt ttaatcacca ttctatttta aaaacagtat gtcatttata
      181 aatgacgccg tttaaaattc gtttagaaaa tacgtttaac gataattaaa aggattctgt
      241 taaatgaaat taaaaatagt tgcggtggtt gtaactggtt tgttagctgc gaacgtagca
      301 cacgctgccg aagtctataa caaggatggt aataaactcg acctttatgg caaggttacc
      361 gctctacgtt attttactga tgataagcgt gacgatggtg ataaaactta tgcccgtctc
      421 ggctttaaag gagaaacgca aatcaatgat caaatgattg gttttggtca ctgggaatat
      481 gattttaaag gctataacga tgaagccaac ggctcgcgcg gcaacaagac ccgtctggcc
      541 tatgctggtt taaaaattag tgaatttggc tctctggact atggtcgtaa ctacggtgtc
      601 ggctacgaca ttggttcatg gaccgatatg ttgccagaat ttggtggcga tacctggagt
      661 cagaaagatg tcttcatgac ataccgtacc accggtctag caacctatcg caactacgat
      721 ttctttggct taattgaagg gctgaacttt gccgcgcaat atcaaggcaa aaatgaacgt
      781 actgacaaca gtcatcttta tggtgctgac tacacgcgtg ccaacggtga cggtttcggt
      841 atctcctcaa cttatgttta tgatggcttt ggtatcggtg cggtatatac caaatccgat
      901 cggacaaatg cgcaggaaag agccgctgct aatcctctca atgcctccgg taagaatgca
      961 gaactatggg ccacaggtat aaagtatgat gccaacaaca tctactttgc agctaattac
     1021 gctgaaacat taaacatgac cacctatggg gatggttata tctctaacaa agcacaaagt
     1081 tttgaagtgg tggcgcaata tcaattcgac ttcggcttgc gcccatcact cgcttacctg
     1141 aaatcgaaag gcagagatct gggccgctac ggcgaccagg acatgattga gtatatcgac
     1201 gttggtgcga cgtatttctt caacaaaaat atgtcgacct atgttgatta taaaatcaac
     1261 ctgattgatg aaagcgactt tacccgtgcc gtagatattc gcaccgataa catcgtcgct
     1321 acgggcatta cctatcagtt ctaacgtcct ctcctgacca gcccttctct gggctggttt
     1381 ttgatttaag tgctgctgta tgactcctta agtaaaaatg agcaattttt gatatttctg
     1441 gtcattcacc tcaattaata ttattccata tcctccgggg acatcttact ataatattta
     1501 ttgatgagtg attatgacca atattaatac agcttgtgta aaaaataatg ccagttatca
     1561 acttaataac gcattaccta acaaagaaac catctccagc aatttttgtg aacgactaga
     1621 acaatggggt aataaatcgg ttaataatgg tgaagaaaga gcaattgccg tggagcgaat
     1681 taaagaagct tacaattcga acatggcatc tcttgatttg tcatatcttg atttgagtga
     1741 attaccgcct atcccctcga cagttaatac gttaaatctg gaaaacaatt gtctaacttg
     1801 tcttgactta gctgacaatg ccagtctcgt taatatcaac ctcagcgtta ataaaattaa
     1861 cacgataacc tttccaaaag aaccaaaact ggaaaacatt tatattgatc ataacaatct
     1921 ggaaagttta gatttaaaaa atcagcattc attggttaat ctggaagcgc aaaataatag
     1981 cctaaaaaaa tgatatttct gatagttata aactgaaatt tcttaatctt gattataaca
     2041 agctagcatc gctggatgtc tcccggcaag aatctctgat tgagttaagt gcccatcaca
     2101 atatgatcac tgaccttata ttacacaatc atcccaggat gaaaacaatc actttaaacg
     2161 acaaccatat tgcacattta aacgccaaaa acactacaaa actggaatat ttaaacttaa
     2221 gcaataacaa tttactgcca accaatgaca ttgatcaact aatatcatca aaacatcttt
     2281 ggcatgtatt agttaacggc atcaacaatg atccacttgc acaaatgcag tactggactg
     2341 cagtaagaaa tataattgat gacactaatg aagtgaccat tgatttatca ggacttaatt
     2401 taaccactca accaccaggg ctgcaaaact tcacctctat caatcttgat aataaccaac
     2461 tcacacattt tgatgcaacc aactacgata gactcgtaaa gctaagtctg aatagtaatg
     2521 ctcttgagtc aataaatttt cctcaaggca gaaatgtaag tattacacat atatctatga
     2581 ataataatgc tctcagaaat attgatatag ataggctttc atcagttact tattttagtg
     2641 cggcacataa tcaactagag tttgtgcaat tagaatcttg cgaatggctg caatacctga
     2701 atctcagcca taatcaatta actgatattg ttgcaggtaa taaaaatgaa ctcttactgc
     2761 tggatctatc ccataataaa ctaacaagtt tacacaatgt cttatttccc aacttgaata
     2821 cgttacttat taacaacaat ttgctttctg aaattaaaat attctatagc aacttccgca
     2881 atgttcagac attaaacgct gctaacaacc agttgaaata tataaatctt gatttcctga
     2941 cttatcttcc atctatcaaa agtttaagac tggacaataa taaaataacc cacattgata
     3001 ctaataatac atccgatatt ggaactttat tccccataat aaaacagagc aaaaacttaa
     3061 attttttaaa tgtttctggg aagaacaatt gccctactat gcagctcatg ttatttaatt
     3121 tgttttcccc agcacttaag cttaatactg gcctggcaat tctttcgcct ggtgcatttg
     3181 aagttcactc tgacggaata gatgtggata acgaattgtt tcactatact attaaaaaag
     3241 catatacccc atataatata cacacttaca agacagagga agttgtaaac cagaggaata
     3301 taaaagttaa aaacatgacc ttagatgaaa taaacaatac ttactataat aacgattatt
     3361 acaatcaggc aataagagag gaaccgatag actttctgga cagatcgttt tcctccaatt
     3421 catggccttt ttagtgaata aatcatcact aattctataa gtgtctggtc ttgttgagaa
     3481 tatagttaat atgtaaccct ttagattcac gatctgaagg gttgaaacat gacatacgtt
     3541 caaagattaa cccccgtttg aaggttagct tgttagttac atttagtaac acatatctgg
     3601 ctacttaatc cccattctgc ccccgcggtt acgctttctt atctattctt tcaatcagca
     3661 cttcacccaa ttcatcctta attctaataa ctggctattg cgccttacct cctgttttgt
     3721 cttttttcat taccaatgtg tgcatgtgag gaacaatatg gcactgcaaa atgagaaaaa
     3781 tagtcgttat cttttacgcg actggaaacc agaaaatccg gccttctggg aaaataaagg
     3841 aaaacatatt gctcgaagaa acctctggat atcagtcagt tgtctacttc ttgccttctg
     3901 tgtctggatg ctatttagcg cagttaccat taatctcaat aaaatcggtt ttaattttac
     3961 taccgatcaa ctctttttat taaccgcatt accctccgtt tctggcgcat tattgcgtgt
     4021 tccctactcc tttatggtgc ctatattcgg tggacgccga tggacggttt ttagtactgc
     4081 aatcctgatt attccttgcg tctggctcgg aattgccgtg caaaatccga atactccttt
     4141 tgggatattt atcgttatcg ctttgcta
//
LOCUS       DBKEKI010000228         4138 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.230, whole genome shotgun sequence.
ACCESSION   DBKEKI010000228 DBKEKI010000000
VERSION     DBKEKI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.230"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..330
                     /gene="uspF"
                     /locus_tag="ACUQPX_004159"
     CDS             <1..330
                     /gene="uspF"
                     /locus_tag="ACUQPX_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HHV9058622.1"
                     /translation="HFLTVIPSLPYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKF
                     KLPTDRVHVHVEEGSPKDRILELAKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAE
                     CSVLVVR"
     gene            506..1441
                     /gene="ttcA"
                     /locus_tag="ACUQPX_004160"
     CDS             506..1441
                     /gene="ttcA"
                     /locus_tag="ACUQPX_004160"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HHV9058623.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1570..2943)
                     /gene="dbpA"
                     /locus_tag="ACUQPX_004161"
     CDS             complement(1570..2943)
                     /gene="dbpA"
                     /locus_tag="ACUQPX_004161"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HHV9058624.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(2973..3146)
                     /gene="ynaL"
                     /locus_tag="ACUQPX_004162"
     CDS             complement(2973..3146)
                     /gene="ynaL"
                     /locus_tag="ACUQPX_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HHV9058625.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3421..>4138)
                     /gene="zntB"
                     /locus_tag="ACUQPX_004163"
     CDS             complement(3421..>4138)
                     /gene="zntB"
                     /locus_tag="ACUQPX_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HHV9058626.1"
                     /translation="LRCINGSTDERPDQLVAMRVYMDGRLIVSTRQRKVLALDDVVSD
                     LEEGTGPTDCGGWLVDVCDALTDHSSEFIEQLHDKIIDLEDNLLDQQIPPRGFLALLR
                     KQLIVMRRYMAPQRDVYARLASERLPWMSDDQRRRMQDIADRLGRGLDEIDACIARTG
                     VMADEIAQVMQENLARRTYTMSLMAMVFLPSTFLTGLFGVNLGGIPGGGWQFGFSIFC
                     ILLVVLIGGVALWLHRSKWL"
BASE COUNT         1089 a         1080 c          994 g          975 t
ORIGIN      
        1 catttcctga cggtaatacc ttcactgccc tactatgcct ctctgggttt agcgtattcc
       61 gcagaattac cggcaatgga tgacctgaaa gcggaagcca aatcgcaact ggaagagatc
      121 attaaaaaat ttaaactgcc aaccgacaga gtgcatgtcc atgttgagga aggctcgccc
      181 aaagaccgca ttctggaatt ggcgaagaag atccccgctc atatgatcat cattgcttcc
      241 catcgaccgg atatcaccac ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac
      301 gcagagtgct ccgtgctggt tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt
      361 ttgattcatt tcgcaaatgt gctgacattt tcccctctaa tccgtaccat acacgccaca
      421 gtttttatat cagatttctt atgctgggcg ttccggcatg acgcatactc ttctgatgcc
      481 atataacgaa ttgagtcgct tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca
      541 atacaacctg aacaaattac aaaaacgtct gcgtcgtaac gtgggcgaag ccattgctga
      601 cttcaatatg attgaagaag gcgatcgcat catggtttgc ctctccgggg gtaaagacag
      661 ctataccatg ctggagattc tgcgcaattt gcagcaaagc gcgccaatca atttttcgct
      721 ggtggctgtt aacctcgatc aaaagcaacc gggcttcccg gaacacgttc tgcccgagta
      781 tcttgaaaag ctgggcgttg agtacaagat tgttgaagag aacacttacg gtatcgtgaa
      841 agagaagatt ccagagggca aaaccacttg ctcactgtgt tctcggctac gtcgcggtat
      901 cctttatcgc accgcaacgg aactggggac gacgaagatc gcgttgggtc accatcgtga
      961 cgatatcctg caaacgttgt tcttaaatat gttctacggc ggcaagatga aaggtatgcc
     1021 tccgaaactg atgagcgatg atggcaaaca tatcgttatt cgtccactgg cctactgccg
     1081 cgagaaagat attcagcgat ttgccgatgc aaaagcgttc ccgattattc cgtgcaacct
     1141 gtgcggttca cagcctaacc tgcaacgtca ggtgattgct gacatgttgc gtgactggga
     1201 taaacgttat ccagggcgta tcgagacgat gttcagcgcg atgcagaatg tggtgccgtc
     1261 gcatctgtgc gataccaacc tgttcgattt caaaggcatt acccacggtt ctgaagtggt
     1321 taacgggggt gatctggcgt ttgatcgcga agagatccca ctacaaccgg cgggctggca
     1381 gccagaagaa gatgaaaatc agttggatga gttacggctg aatgtggttg aagtgaaata
     1441 accaggatag cgcccgatgc gcaagcgtat cgggctactc ttatggaggc cggataagac
     1501 gcggccagcg tcgcatccgg caatcccgaa taagatgttt actcttgcac ccggcaattc
     1561 aacatttcat tattttaata accgcacccg gcacgttttt cctttaatct tcccgccctg
     1621 taactgtttc catgctttat gagcaacagc ctgacggacc gcgacataga catgcgccgg
     1681 atgcacggcg attttgccaa tatctgcgcc atcaagcccg atatctcctg tcagtgcacc
     1741 taatacatca cccgggcgca ttttggcttt tttcccgcca tcgatacaca acgttgccat
     1801 ttctgcttcc agcgtcacaa tggaactatt agctggcggc gtttgccagt taagttttat
     1861 ctgcaacatg tcagaaatga tattggcccg ctgtgcttct tccggagcac agaaactgat
     1921 cgccagaccg ctatttcctg cacgagctgt acgaccgatg cgatgtacat gaacttcagg
     1981 gtcccacgcc agctcaaagt tcaccaccag ctcaagcgat ttaatatcca gaccacgcgc
     2041 agcaacatca gtcgcaacca gtacacgggc gctaccgtta gcaaaacgta ccagggtctg
     2101 atcgcgatcg cgttgctcca gatcgccgtg taacgacaat gcactttgct ctacttcatt
     2161 cagcgcgtcg cagacagcct ggcaatcttt tttggtattg caaaacacca cacaagagga
     2221 tggctgatgc aagcttaata accgttgcaa cagaggaatt ttgcctttac tggatgtctc
     2281 ataaaattgt tgttcaatgg gtggcaaagc atctgttgag tcaatttcaa tcgccaaagg
     2341 atcgcgttgc actcgtccgc tgattgcggc gatggcttcc ggccaggttg ccgaaaacag
     2401 aagcgtctgt cgagatgcag gcgcaaaacg gatgacatca tcaatggcat cgctaaatcc
     2461 catatccagc atgcggtcgg cctcatccat caccaaagta ttcagcgcat ccagtgatac
     2521 cgtgcctttt tgcaagtgat ccagcaaacg acccggcgtt gccacgataa tatgcggcgc
     2581 atgttgcaac gaatcacgct gcataccgaa cggttgacca ccgcacaacg tcaaaatttt
     2641 ggtatttggc agaaaacgcg ccagccgacg caattcacct gccacctgat ccgccagttc
     2701 acgcgtagga cacagcacta aagcctgggt ttgaaatagc gacgcatcaa tttgctgtaa
     2761 caagccgagg ccaaaagccg ccgttttgcc gctgccggtt ttcgcctgca cgcgaacatc
     2821 ttttccggca aggatcgccg gaagcgcggc ggcctgcacc ggcgtcatgg ttaaataacc
     2881 caactcatta aggttcgtga gttgggcggg aggcaaaaca ttcagggtag aaaaagcggt
     2941 cacaatctat tctcgtggtc atcgacgcaa agttagcagg cgcgtatcct cgcagatcta
     3001 cgctcacgat gcgacaattt aatcggttct tcatcgggtg gtgggtcagg catgggttgc
     3061 gggcgaggga tcggatcggg cactggaaca ggatcgccag gaatcggttc agggacagga
     3121 atttgcaaat aaataagtgt cgtcatattt ccctccggtc attgggtgga ctcttaaagg
     3181 gtagacgctg ataaataaca ggcaaaaaaa agccgactca tcaaagtcgg cgtcgtacga
     3241 atcaattgtg ctatgcagta attcaaaaaa ggaagtaaga caatatggag cgcaacgccc
     3301 atcgcttgac gttgcattca cctgcaaggg agatattgcc ctgaatgggt agagagttta
     3361 ttgacttcgc tcaaactttg cggcgttttt gtatacagac agccggaaaa attgcttttg
     3421 ttacaaccat ttactacggt gcaaccataa agcaacacca ccaataagaa caactaacag
     3481 aatacaaaaa attgaaaatc cgaattgcca cccgccgcca gggatcccac caaggttgac
     3541 gccaaataac ccggtcagaa aggtactggg taaaaagacc attgccatca acgacattgt
     3601 ataggtacga cgagctaaat tttcctgcat cacctgagcg atttcatccg ccatcacgcc
     3661 agtccgtgct atacaggcgt cgatttcgtc aaggccgcgc ccaaggcgat cggcaatatc
     3721 ctgcatccga cggcgttggt catcgctcat ccacggcaaa cgttcactgg caagacgagc
     3781 ataaacatca cgttgcggtg ccatatagcg acgcatcaca attaattgtt tgcgcagcag
     3841 agccaagaat ccacgcggtg gaatttgctg atcaaggaga ttatcttcaa ggtcgataat
     3901 tttatcgtgc agctgctcga taaattcact ggaatgatcg gtcaacgcat cgcacacatc
     3961 caccagccat cccccgcaat cggtcggacc cgtgccctct tccagatcgc tcaccacatc
     4021 gtccagcgcc agcactttgc gttgtcgggt cgaaacaatt aaccgcccgt ccatatatac
     4081 acgcatggcg accagttgat cggggcgttc atcggtgctg ccgtttatac agcgcaat
//
LOCUS       DBKEKI010000229         4127 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.231, whole genome shotgun sequence.
ACCESSION   DBKEKI010000229 DBKEKI010000000
VERSION     DBKEKI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.231"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            82..354
                     /locus_tag="ACUQPX_004164"
     CDS             82..354
                     /locus_tag="ACUQPX_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058627.1"
                     /translation="MDNNTISLQELLDCISRLRDDVNALTVAFSSLALSIPREQMQPT
                     LASLLLESRNPKWSQEQQNSFKWLAALLEEKYAGKITISVESSENQ"
     gene            complement(320..664)
                     /locus_tag="ACUQPX_004165"
     CDS             complement(320..664)
                     /locus_tag="ACUQPX_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HHV9058628.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARMSIRQVFLDVAAGLCEGDEQSPEKRPIILEAQNVWITYKGKLPG
                     RITDSLKTPPKW"
     gene            complement(669..884)
                     /locus_tag="ACUQPX_004166"
     CDS             complement(669..884)
                     /locus_tag="ACUQPX_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HHV9058629.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(1034..1195)
                     /locus_tag="ACUQPX_004167"
     CDS             complement(1034..1195)
                     /locus_tag="ACUQPX_004167"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058630.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(1195..1380)
                     /locus_tag="ACUQPX_004168"
                     /pseudo
     CDS             complement(1195..1380)
                     /locus_tag="ACUQPX_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            1640..1975
                     /locus_tag="ACUQPX_004169"
     CDS             1640..1975
                     /locus_tag="ACUQPX_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HHV9058631.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(2256..2342)
                     /locus_tag="ACUQPX_004170"
     CDS             complement(2256..2342)
                     /locus_tag="ACUQPX_004170"
                     /inference="COORDINATES: protein motif:HMM:NF024464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HHV9058632.1"
                     /translation="MSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2887..2963)
                     /locus_tag="ACUQPX_004171"
     tRNA            complement(2887..2963)
                     /locus_tag="ACUQPX_004171"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2927..2929),aa:Arg,seq:tct)
     gene            complement(3064..3139)
                     /locus_tag="ACUQPX_004172"
     tRNA            complement(3064..3139)
                     /locus_tag="ACUQPX_004172"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3104..3106),aa:Ile,seq:cat)
     gene            complement(3283..4104)
                     /locus_tag="ACUQPX_004173"
     CDS             complement(3283..4104)
                     /locus_tag="ACUQPX_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HHV9058633.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT         1169 a         1099 c          771 g         1088 t
ORIGIN      
        1 ttctgaaccc tggatatgtt tatctgtccc agcctgttga atcaggctgg gaaaaggtaa
       61 agacaatcaa gaggattatt tatggacaat aacaccattt ctctacagga gttgctcgac
      121 tgcatttcca ggcttcggga tgatgtaaat gccctcactg ttgcattttc atctctggcc
      181 ttatcaattc ccagagaaca aatgcaacca acactggcat cgctcctgct tgaatcacgc
      241 aaccccaaat ggtcccagga acaacaaaat tctttcaagt ggctggcggc attactggaa
      301 gaaaaatatg ctggtaaaat taccatttcg gtggagtctt cagagaatca gtaattcttc
      361 ctggtagctt tcctttgtag gttatccaca cattctgcgc ctctaaaatt atggggcgct
      421 tttccggcga ctgctcatcc ccttcacata acccggcagc aacatccagg aagacctgtc
      481 tgatgctcat tctggctgct gcctcataaa actccagcgc ggcaccttca acacggtcca
      541 gcgagatgtc caggtcaaaa atttcaccgt caaagcgttt tttgtcccgt aacgctaaag
      601 ttaccgtaac tttattctca aaattgcgga tccctttcac aatcagttca tagttttgag
      661 tcattgaatt actctccccg tgcagcctta cgacggtcct ctctgatttt gaaatacagg
      721 ttagtcagat atgtcagcag cccaaacagc agactcccca gcacgcctat tgccgcccac
      781 tgagacgggg aaaccctgtc cagcaactgc aggaaccagt agcccgttcc caccgctgac
      841 gtggtgtatg acacacctgt tgtgattttt tccatctggt ccataccccg tctcccgtta
      901 tccggaagct gacaacaata aaaaagccac cagttaacta ctgatggctc tgataactca
      961 tgcaagcgtc tcagacgacc cactgacact accggtgagt ttaacgatac cttccatttg
     1021 actggctcac tttttatgat gatgccggtg catttatctc cagcaccaga ctttctatct
     1081 caacgccata cgttgcattt ttggtaatat ccgtcagcgt cagtgcattt agtcccactg
     1141 ccagactgtc ttttatggcc tggaatgccg ggccagtacg atgacgtagt atcactccgg
     1201 ctcagttgca ccactgacca ccacatcacc ttctgctgca atcgcctgca tcagggtata
     1261 aggggttatg gccaccggac taccaaacgg ctgccagccc tctttcagtt tatgtgtcag
     1321 cttttccgca agatctgacg gcggcgccgc cctgacaaca tcatagtgtt taaatgccat
     1381 ggttctttcc accatctgaa aaataattct ttaaaatacc tgacatgtaa tacagaaaaa
     1441 acacaaaacc ataccttaaa taaaaacctg attatcaagc agatatgcat ggataaacta
     1501 caagacgaga tataaaccac cctgcattta aataaacaat aaacaacatc agaaaaataa
     1561 ttctgctcta tggtttaatt caaaaatatc atttatactt ttcagaacat caccagcaag
     1621 gcataaacaa ggaaactaaa tgaagtggat tgtgattgat acagttatcc agccatcatg
     1681 cggaatatct ttttcagtca tatggagtaa aataaaatta ataatctggt atcaatcgga
     1741 tgctttctta cctcctgaaa gtatatttac actgactcac acaggcatca tgctcaataa
     1801 caaagtgcta cctgtaacca tttacaacgt agtaccattc aataaaacat tctggaattt
     1861 aatcaaaaac agccaggaat gccctacaaa tacagataac gtattgaatg aatgctttaa
     1921 taaccgttgc actctgcaaa tatgtcctta tggactaaaa caacaaagtc cataaggagt
     1981 ttactcacat ctgacaaaat caatataaac agcccctccg gagaggggct ggagagtggc
     2041 gctatgtgcc attgcatggt gccgggtgcc tcccggtgaa ttcagtacca gcacctgaat
     2101 ccgcgattat cccatatacc tactcgctga ttgcccctcc gcacaggggg attcaccatg
     2161 ccagtttctt ttaacaaact cctcgcaaac cagacaacag tcaaccgcct gaattgtgaa
     2221 gtatttaaaa atttctcccg ctaactgata cccggctaac agtctggcgt tttctttttc
     2281 agcaacggga aagcaacaac caccacaccc gccaccagca caccgtcagc cggcactgac
     2341 attatccggc tgctgcaatg ccattcacaa aaacagtaag caatcacttt ttaccgtaac
     2401 aggtgataat ccagatatgt atctacccca gatgagtaat ccgaagttca tccataccac
     2461 aggtcctggc tattctgttg tactcctgaa caagagcaaa taattctgaa ttagcaacca
     2521 tgaactcatc gcaaaccctc tgtatagcat cactattcag aataataacg tctcttcccg
     2581 aaagacgatc aggagtacag aacaaaactg tcaaacggct gaaggccttt gctcgtgctg
     2641 cattgactat atcaatacgc tgcctaagga tgaaacaccc cgacgcctca tcaatattca
     2701 ctctacccac accatatgaa tgataaatac ttaatgctga aaaaaccatt agaccgtata
     2761 acaaatactc aatcaatact taacagaact tttatttttg acaaacatat aatattttca
     2821 acaatatcct gagccaggta tatttcagta taaggctctg ccggaaggaa tctggaagaa
     2881 tgaatatggc gcgctgtact ggattcgaac cagtgaccga ttgcttagaa ggcaattgct
     2941 ctgtccggct gagctaacaa cgctgaatac cgataatgga ccgccatcgg ggacccgccc
     3001 ccgcaccaac aaccctgtta tcgtgtcgtc tgctcttcct gataagctaa tggcggtttg
     3061 tgatggtggc ccttgctgga tttgaaccag cgacctggcg attatgagtc gctcgctctc
     3121 accactgagc taaagggccg ggagcagaat aataacggtc cgtaattaat ttcgcaataa
     3181 aaaacccgct cagcggcggg ttgtagaaac tcttctaacg tcaggcataa aacgcccatc
     3241 attatgacga atttaccaca gattccggaa aaatcaacct tgttacctag ttaccttttt
     3301 taactgccgc tcagcccatg cttcttcaat atcaaaccgg gtcaccagcg catcataaaa
     3361 tttcttaact gttttttccc atgacgcgcg tgttatctgg tttgtcacct cgcatatagc
     3421 attaaatgcc tccgttgatg gtagtctttc atagccacga ccaccacaac gctggcagtc
     3481 tctgataaca ggcataccac gttttaccga ctcttcacga tgaatggcga caccacgccc
     3541 acggcaatcc ttacaggcgg tggaaacctc accctttccg ccacactccg gacaggcaac
     3601 ttttaccacc tccctgactt ttttccattc ttcccagtaa gacggataca cacctttcgt
     3661 acactttgcc cataccggcg gcttaccatc cggatactgg atcttatttg taaaaactat
     3721 gctttcaata aatttttccc catagcaaca agggcactgc tttttactcg ctgcgctgcg
     3781 ggcataatcc tcaaaagcgt acgaagccat aatgcgcatc actaccggtt ttatttctgc
     3841 cggaagtttt ctcaacgccg ccacacgatc gcaccgactg agtgcataat ctgccagtaa
     3901 ttctgttgcc cgcgccctgt cattcatact gatgcccatt ttccccagga acgcagaaaa
     3961 acccatctca gcccgattct gtgtcatgcc ctgcgcggcc atcacatcag tgatactcag
     4021 cgcatctttt gacgttgagg ccgatgcatc ggtcaggcca ggggattttg gggagtagta
     4081 tttcggtaaa tcttccagtt tcattttttg acctgctctt aatgcat
//
LOCUS       DBKEKI010000230         4099 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.232, whole genome shotgun sequence.
ACCESSION   DBKEKI010000230 DBKEKI010000000
VERSION     DBKEKI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.232"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            127..1533
                     /gene="fliD"
                     /locus_tag="ACUQPX_004174"
     CDS             127..1533
                     /gene="fliD"
                     /locus_tag="ACUQPX_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HHV9058634.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     ALAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            1558..1956
                     /gene="fliS"
                     /locus_tag="ACUQPX_004175"
     CDS             1558..1956
                     /gene="fliS"
                     /locus_tag="ACUQPX_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HHV9058635.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSI"
     gene            1968..2333
                     /gene="fliT"
                     /locus_tag="ACUQPX_004176"
     CDS             1968..2333
                     /gene="fliT"
                     /locus_tag="ACUQPX_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HHV9058636.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            2411..3898
                     /gene="amyA"
                     /locus_tag="ACUQPX_004177"
     CDS             2411..3898
                     /gene="amyA"
                     /locus_tag="ACUQPX_004177"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HHV9058637.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(3932..>4099)
                     /locus_tag="ACUQPX_004178"
     CDS             complement(3932..>4099)
                     /locus_tag="ACUQPX_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HHV9058638.1"
                     /translation="MSDDLTNVTVKRELYEVERDGNTIEYDGMTMERVDRPTAECAAA
                     LDKAPLPTPLP"
BASE COUNT         1110 a         1042 c         1036 g          911 t
ORIGIN      
        1 gacgaaatac tcttttctct gccccttatt cccgctatta aaaaaacaat taaacgtaaa
       61 ctttgcgcaa ttcaggccga taaccccggt attcgtttta cgtgtcgaaa gataaaagga
      121 aatcgcatgg caagtatttc atcgctggga gtcgggtcag gtctggattt aagttcaatc
      181 cttgatagcc tcaccgccgc gcaaaaagcg acgctaaccc ctatttcaaa tcagcaatcg
      241 tcgtttaccg ctaaacttag cgcctacggt acgctgaaaa gcgcgctgac gactttccag
      301 accgccaata ctgcattgtc taaagccgat cttttttccg ccaccagcac caccagcagc
      361 accaccgcgt tcagtgccac caccgcgggt aacgccatcg ccgggaaata caccatcagc
      421 gtcacccatc tggcgctggc gcaaaccctg accacgcgca ccaccagaga tgatacgaaa
      481 acggcgatcg ccaccagcga cagcaaactc accattcaac aaggcggcga caaagatccg
      541 attaccattg atatcagcgc ggctaactca tcgttaagcg ggatccgtga tgccatcaac
      601 aacgcaaaag cgggcgtaag tgcgagcatc attaacgtgg gtaacggtga atatcgtctg
      661 tcagtcacat caaatgacac cggccttgat aatgcgatga cactctcggt cagcggtgat
      721 gatgcgctac aaagttttat gggctatgac gccagtgcca gcagcaacgg tatggaggtc
      781 tcggtcgccg cccagaatgc gcagctgacg gtcaacaacg tcgccatcga gaacagcagc
      841 aacactatca gcgacgcgct ggaaaacatc accctgaacc tgaacgatgt caccacgggc
      901 aaccagacgc taaccatcac tcaggacacc tccaaagcgc aaacggcgat taaagactgg
      961 gtgaatgcct ataactcgct aatagatacc ttcagcagcc tgaccaaata caccgccgta
     1021 gatgcgggag ctgatagcca gagttctagc aatggcgcac tgctcggcga ctccacgctg
     1081 cggacgattc agacgcagtt gaagtcgatg ctgagtaata ccgtcagttc ttccagctat
     1141 aaaacgctgg cgcagattgg tatcacgacc gatcccagcg atggcaaact ggaactggat
     1201 gccgacaaac tcaccgctgc actgaaaaaa gatgccagcg gcgtaggtgc attgattgtt
     1261 ggcgatggta aaaaaaccgg catcacaacc accatcggca gcaacctgac cagttggctt
     1321 tcgacaacgg gcattattaa agccgctacc gatggcgtta gtaaaaccct gaataagtta
     1381 actaaagact acaacgccgc cagcgatcgc attgatgcgc aggtcgcgcg ctacaaagaa
     1441 caatttaccc aactggacgt tttaatgacc tcgttaaaca gcaccagcag ctacttaacg
     1501 cagcagttcg aaaacaacag taattccaag taatcattat tcatcgggag acaggtcatg
     1561 tacgcggcaa aaggcaccca ggcctatgca caaattggcg tcgaaagcgc cgtaatgagc
     1621 gccagccaac agcagttggt caccatgcta tttgatggag tgctgagcgc actggttaga
     1681 gcgcgactgt ttatgcagga caacaatcag cagggcaaag gcgtctcttt gtcaaaagcg
     1741 atcaacatca ttgagaacgg actgcgggtg agtcttgatg aagagagcaa agacgaatta
     1801 acccaaaact taattgctct ttatagctat atggtcaggc gtttgctgca agccaattta
     1861 cgcaacgatg tctccgcagt cgaagaggtg gaagcattaa tgcgtaatat tgccgatgcc
     1921 tggaaagagt ctttactctc cccttcttcg atttaggacc cagtctgatg aacaatgcac
     1981 cgcatttgta tttcgcctgg caacaactcg tcgaaaaaag ccagctcatg ttacgcctgg
     2041 caacggaaga acaatgggac gaactcatcg ctagcgaaat ggcgtatgtg aatgcggtgc
     2101 aggagatcgc acacctgact gaagaggttg ccccgtccac tacgatgcag gagcagctcc
     2161 gcccgatgct gcacctgatt ctcgacaacg aaagcaaggt aaagcagtta ttacagattc
     2221 ggatggatga actggcgaaa ctggtcggtc agtcatcggt gcaaaaatcg gtgttaagtg
     2281 cctatggcga tcagggcggc tttgtgctgg ctccgcagga taacctcttt tgaatcttaa
     2341 tgagccgatg gctcgcgaat aatccgatta cggctacgct tctaatattc cccttgaatg
     2401 gagtcgaaga atgcgtaatc ccacgctgtt acaatgtttt cactggtatt acccggaagg
     2461 cggtaagctc tggcctgaac tggccgagcg cgcagacggt tttaatgata ttggtatcaa
     2521 tatggtctgg ttgccgcccg cctataaagg cgcatcgggc ggctattcgg tcggctacga
     2581 ctcctatgat ttatttgatc taggtgagtt tgatcagaaa ggcagcatcc ctactaaata
     2641 tggtgataaa gcacaactgc tggccgccat tgacgccctg aaacgtaatg acattgcggt
     2701 gctgctggac gtggtagtca accacaaaat gggcgcggat gaaaaagaag ctattcgcgt
     2761 gcagcgtgta aatgctgatg accgtacgca aattgacgaa gaaatcattg agtgtgaagg
     2821 ctggacgcgt tacaccttcc ccgctcgtgc cgggcaatac tcgcagttta tctgggattt
     2881 caaatgtttt agcggtatcg accatatcga acaccctgat gaagatggca tttttaaaat
     2941 tgttaacgac tataccggag aaggctggaa cgatcaggtt gatgatgaac tcggtaattt
     3001 cgattatctg atgggtgaga atatcgattt tcgcaatcat gccgtgacgg aagagattaa
     3061 atactgggcg cgctgggtga tggaacaaac gcaatgcgac ggctttcgtc ttgatgcggt
     3121 caaacatatt ccggcatggt tttataaaga gtggatcgaa cacgtacagg aagttgcgcc
     3181 aaagccgctg tttattgtgg ctgaatactg gtcgcatgaa gtcgataaac tgcaaacgta
     3241 tattgatcag gtggaaggca aaaccatgct gtttgatgcg ccgctgcaga tgaaattcca
     3301 tgaagcatcg cgcatgggtc gcgactacga catgacgcag atcttcactg ggacgctggt
     3361 ggaagccgat ccttttcacg ccgtaaccct cgttgccaac cacgacaccc aaccgttgca
     3421 agccctcgaa gcaccggtcg aaccgtggtt taaaccgctg gcgtatgcct taattttgtt
     3481 gcgggaaaat ggcgttcctt cggtgttcta tccggacctc tacggtgcac attacgaaga
     3541 tgtcggtggt gacgggcaaa cctatccgat agatatgcca ataatcgaac agcttgatga
     3601 gttaattctc gcccgtcagc gtttcgccca cggtgtacag acattatttt tcgaccatcc
     3661 gaactgcatt gcctttagcc gcagtggcac cgacgaatat cccggctgcg tggtggtcat
     3721 gtcgaacgga gatgatggcg aaaaaaccat tcatctggga gagaattacg gcaataaaac
     3781 ctggcgtgat tttctgggga accggcaaga gagcgtagtg acagacgaaa acggcgaagc
     3841 gaccttcttt tgcaacggcg gcagcgtcag cgtgtgggtt atcgaagagg tgatttaaat
     3901 ccatccccgg cggcaagccg gggagatttc attacggcag tggcgtcggt aacggcgctt
     3961 tatccagcgc ggcggcacac tctgccgtcg ggcgatccac acgctccatc gtcataccat
     4021 cgtactcaat ggtgttaccg tcacgctcaa cctcgtacag ctcacgtttt acggtaacgt
     4081 tagtgagatc atcagacat
//
LOCUS       DBKEKI010000231         4094 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.233, whole genome shotgun sequence.
ACCESSION   DBKEKI010000231 DBKEKI010000000
VERSION     DBKEKI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.233"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            2..766
                     /gene="cheR"
                     /locus_tag="ACUQPX_004179"
     CDS             2..766
                     /gene="cheR"
                     /locus_tag="ACUQPX_004179"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HHV9058639.1"
                     /translation="MIYQRAGIVLADHKRDMVYNRLVRRLRSLGLTDFGHYLNLLESN
                     QHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYRVWSAAASTGEEPYSIA
                     MTLADTLGTVPGRWKVFASDIDTEVLEKARSGIYRHEELKNLTPQQLQRYFMRGTGPH
                     EGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMIYFDQTTQQEILRRFVP
                     LLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD"
     gene            769..1818
                     /gene="cheB"
                     /locus_tag="ACUQPX_004180"
     CDS             769..1818
                     /gene="cheB"
                     /locus_tag="ACUQPX_004180"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HHV9058640.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            1833..2222
                     /gene="cheY"
                     /locus_tag="ACUQPX_004181"
     CDS             1833..2222
                     /gene="cheY"
                     /locus_tag="ACUQPX_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HHV9058641.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            2233..2877
                     /gene="cheZ"
                     /locus_tag="ACUQPX_004182"
     CDS             2233..2877
                     /gene="cheZ"
                     /locus_tag="ACUQPX_004182"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HHV9058642.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(3024..3716)
                     /locus_tag="ACUQPX_004183"
     CDS             complement(3024..3716)
                     /locus_tag="ACUQPX_004183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058643.1"
                     /translation="MNVVSNTQQLEQRIADFFTLSDEHKKARVLLDTLACSCPAWIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFREKLIFCEDESSLLNTTLMSWDAVLYDLRSEKIISKDNYLTDLNRRH
                     LELVLHTNPNPSGSVTKILRTIFTKQVKSLGPELCHFLHHELTKNVMKYGITTMYCTT
                     SHRHFLVHNLMH"
     gene            complement(3685..>4094)
                     /locus_tag="ACUQPX_004184"
     CDS             complement(3685..>4094)
                     /locus_tag="ACUQPX_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HHV9058644.1"
                     /translation="KMFDRTRGYLHRSVLQQELRGSNYITNLMDMLWIGIHGHKTKKQ
                     RDGERESASADNLFVSREEYLSDTPFGRYAYGTISENYRRVFEDPANTLPVLYKEAQL
                     LTDAISGMTDSYLMRLHDELKSLYECGLKHPTA"
BASE COUNT         1035 a          932 c         1130 g          997 t
ORIGIN      
        1 attgatctat caacgagccg ggatcgttct ggctgaccat aaacgcgaca tggtttacaa
       61 ccgactggtt cgtcgtttgc gttcgctggg actgacggat ttcggtcatt atctgaactt
      121 gctggaatct aatcagcaca gcggtgagtg gcaggcgttt atcaattcgc tgaccacgaa
      181 tctgacggca tttttccgtg aggctcatca tttccctctg ctcgcggatc acgcacgtcg
      241 ccgttctggc gagtatcgcg tatggagcgc ggcggcttcg accggcgaag agccgtacag
      301 cattgcgatg actctggctg acacattggg caccgtgccc ggacgctgga aagtgtttgc
      361 cagtgatatc gacaccgaag tgctggaaaa agccagaagc ggtatctatc gccatgaaga
      421 gttgaaaaac ctgacgccgc agcaactgca acggtatttc atgcgaggga cgggaccgca
      481 tgaagggctg gtacgcgtgc gtcaggagct ggcgaactat gttgattttg ccccgctgaa
      541 tttactggcg aaacagtaca ccgtgccggg gccgtttgat gcgatcttct gtcgtaacgt
      601 catgatctac ttcgatcaaa ctacccagca ggagattttg cgccgctttg ttccgctcct
      661 taaacccgac ggattgctgt ttgcgggtca ctctgaaaac tttagccacc tcgagcgccg
      721 cttcacgctg cgtggtcaga cggtgtatgc gctaagtaag gattaacgat gagcaaaatc
      781 agggtgttat ctgtcgatga ttcggcactg atgcgccaga tcatgacaga aatcatcaac
      841 agccatagcg acatggaaat ggtggcgacc gcgcctgatc cgctggttgc gcgtgacttg
      901 attaagaaat tcaatcccga tgtgctgacg ctggatgttg aaatgccgcg gatggacgga
      961 ctggatttcc tcgaaaaatt aatgcgtttg cgtccaatgc ccgttgtgat ggtttcttcc
     1021 ttgaccggca aaggatcaga agtcacgctg cgcgcgctgg agctgggggc aatagatttt
     1081 gtcaccaaac cgcaactggg tattcgcgaa ggaatgctgg cgtatagcga aatgattgct
     1141 gaaaaggtgc gtacggcagc aaaggcgagc cttgcagcac ataagccatt gtcagcaccg
     1201 acaacgctga aggcagggcc gttgttgagt tctgaaaaac tgattgcgat tggtgcttca
     1261 acgggtggaa ctgaggcaat tcgtcacgta ctgcaaccgt tgccgctttc cagcccggca
     1321 ttgttaatta cccaacatat gccgcccggt ttcacccgct cttttgccga cagacttaat
     1381 aagctttgcc agatcggggt taaagaagcc gaagacggag aacgtgtctt gccggggcat
     1441 gcctatattg cgccgggcga tcggcatatg gaactggcgc gtagtggcgc aaattatcaa
     1501 atcaaaattc acgacggccc ggcggttaac cgtcatcggc cttcggtaga tgtgttgttc
     1561 cattctgtcg ccaaacaggc ggggcgtaat gcggttgggg tgatcctgac cggtatgggc
     1621 aacgacggcg cggcgggaat gttggcgatg cgtcaggcgg gggcatggac ccttgcgcaa
     1681 aacgaagcaa gttgcgtggt gttcggcatg ccgcgcgagg ccatcaatat gggtggtgtc
     1741 tgcgaagtgg tcgatcttag ccaggtaagc cagcaaatgt tggcaacaat tagtgccgga
     1801 caggcgatac gtatttaaat caggagtgtg aaatggcgga taaagaactt aaatttttgg
     1861 ttgtggatga cttttccacc atgcgacgca tagtgcgtaa cctgctgaaa gagctgggat
     1921 tcaataatgt tgaggaagcg gaagatggtg tcgacgctct caataagttg caggcaggcg
     1981 gttatggatt tgttatctcc gactggaaca tgcccaatat ggatggcctg gaattgctga
     2041 aaacaattcg tgcggatggc gcgatgtcgg cattgccagt gttaatggtg actgcagaag
     2101 cgaagaaaga gaacatcatt gctgcggcgc aagcgggggc cagtggctat gtggtgaagc
     2161 catttaccgc cgcgacgctg gaggaaaaac tcaacaaaat ctttgagaaa ctgggcatgt
     2221 gaggatgcga ctatgatgca accatcaatc aaacctgctg acgagcattc agctggcgat
     2281 atcattgcgc gcatcggcag cctgacgcgt atgctgcgcg acagtttgcg ggaactgggg
     2341 ctggatcagg ccattgccga agcggcggaa gccatccccg atgcgcgcga tcgtttgtac
     2401 tatgttgtgc agatgaccgc ccaggctgcg gagcgggcgc tgaacagtgt tgaggcgtca
     2461 caaccgcatc aggatcaaat ggagaaatca gcaaaagcgt taacccaacg ttgggatgac
     2521 tggtttgccg atccgattga ccttgccgac gcccgtgaac tggtaacaga tacacgacaa
     2581 tttctggcag atgtacccgc gcataccagc tttactaacg cgcaactgct ggaaatcatg
     2641 atggcgcagg attttcagga tctcaccggg caggttatta agcggatgat ggatgtcatt
     2701 caggagatcg aacgccagtt gctgatggtg ctgttggaaa acatcccgga acaggagtcg
     2761 cgtccaaaac gtgaaaacca gagtttgctt aatggacctc aggtcgatac cagcaaagcc
     2821 ggcgtggtag ccagtcagga tcaggtggac gatttgttgg atagtcttgg attttgattt
     2881 gtattgcctg aacttcggcc tgcgcgatga tgccaggccg atagcagagt ggcgggtatc
     2941 gttcagggag tacgtggagg atggttattt gtagtttgat aattttagat cagcgaagcg
     3001 tgattgttgc gccagtaaat ttatcaatgc attaaattgt gaacgagaaa atgacgatga
     3061 ctggtagtgc agtacatcgt agtgatgcca tacttcataa cgtttttcgt cagttcatgg
     3121 tgcaagaaat gacagagttc cggccctaaa ctctttacct gcttagtaaa aatagtgcgt
     3181 aaaatcttcg ttaccgaacc gcttggattg gggttagtat gaagaacaag ttcaagatgt
     3241 ctgcgattta agtcggtcaa gtaattatct ttactgataa ttttttcact acgtagatca
     3301 tagagcacgg catcccaact cattagcgta gtgtttaaca gacttgattc atcctcacaa
     3361 aatataagtt tttcccggaa tgcccatgtc tcattgaggt tccagatatc aaattcgaag
     3421 tcgtgataac gaaaacggat cccaccaaac ttattaaaac gcagttgttt gacgggcagt
     3481 tcagccaatg tctggaagag ttcctcacgg gagcgaccga tgacgatatc aaggtctgaa
     3541 gagaatccat cgacgccata caagcctaag tcacgcacca tcccgccaaa aatccaggcc
     3601 ggacaggaac aggccagagt atcaagtaat actctggctt tcttatgctc atcactaagc
     3661 gtaaaaaaat cggcgatacg ctgttcaagc tgttgggtgt ttgagaccac attcatacag
     3721 tgattttagc tcatcgtgaa ggcgcattaa ataactatcc gtcatgcctg agattgcgtc
     3781 ggtaagcaac tgcgcttctt tataaagaac agggagtgta ttggcgggat cttcaaacac
     3841 gcggcgataa ttttcagata ttgtcccgta agcatagcga ccaaagggag tatccgacag
     3901 gtattcttca cgagaaacaa aaaggttatc tgcagacgcg gactcgcgtt ctccgtctcg
     3961 ttgtttcttt gttttgtgtc catgaatgcc aatccagagc atatccatca agttagtaat
     4021 gtaattagac cccctaagtt cctgttgcaa gactgaacga tgaaggtagc ctcgtgttcg
     4081 gtcgaacatt ttca
//
LOCUS       DBKEKI010000232         4050 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.234, whole genome shotgun sequence.
ACCESSION   DBKEKI010000232 DBKEKI010000000
VERSION     DBKEKI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.234"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..395)
                     /locus_tag="ACUQPX_004185"
                     /pseudo
     CDS             complement(1..395)
                     /locus_tag="ACUQPX_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            734..925
                     /locus_tag="ACUQPX_004186"
                     /pseudo
     CDS             734..925
                     /locus_tag="ACUQPX_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233446.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1085..2794)
                     /locus_tag="ACUQPX_004187"
     CDS             complement(1085..2794)
                     /locus_tag="ACUQPX_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HHV9058645.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(3227..3946)
                     /locus_tag="ACUQPX_004188"
     CDS             complement(3227..3946)
                     /locus_tag="ACUQPX_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HHV9058646.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1171 a          835 c          742 g         1302 t
ORIGIN      
        1 ggcatgctgt acccggtcgt aataaccgct cctggataca ccaggtaccc gacacatcgc
       61 ttcaacaggg aattgttgtc gccattgttc gactaacgcg tatttttcag cgactcctgg
      121 gcaaaatacg ctgttgcttt ttttaatata tctcgctcaa gtcgtgcttc atttaaagcc
      181 ttacgcagtt gcataacttc agattccagc tcagccactg tgcgggagcc tggtgtattg
      241 agcccttttc ttgcggcggt aacccattgt cctaaagtgc cttcaggaac agataatcgg
      301 gaagcgcctt cactgatcga aagctgattt tcaggaaccg ttttgacggc ttcggcttta
      361 aactccgtag agtaacgttg gttttttctg ctcattattg gctccttctg atgccattct
      421 atttcaggaa ggagtgtccg ttaaactcag gctacctcag ataagcttga tacaactggt
      481 ctggtatata cagtttcttt gagtgcggtt acgtccacag aggtaacggt aaaagtaacc
      541 ccttgtcggg accactccga ccgcgcacag aggcattttc atcgctgaca ggccgcattg
      601 cctcgcaggg ctggaggagg atcggtgata tccggacgac cgtaccggct tacgccgggc
      661 ttcggcggtt ttactcctct gccatgtgca acaacggagt gccgccttac atgccgctgg
      721 cgcggcatat cctggtaaca tttttgttga taccaccttc cggccttatc ctgaccagtg
      781 ggctttcctg gcatcgatga taccgatgaa cgcgcatgat attgagccga cgatattacg
      841 ggcaactgga aatactcacc ctctggatgt gacctttatc catgaagaag atttggccac
      901 accatggaaa cctgaacaat catcagttta cgcccatgta aacccctacg ggatatttga
      961 actcgatatg gaaacaaggt tgccaatcga ggtggttgct tgaaagttgg cagttacccg
     1021 gtcactacac cttgttggag tgaacatatc aaaaacaggt aattacacaa tttaaattac
     1081 agtctcataa aaaatgatta aatctattta ttatttcaat aaacgttttt ttcatcttct
     1141 ttgaaaattg ttctgctata gtatccggtt ccagattgtt ctcttttatt attctacaat
     1201 actctaatat tgtttctttg tctctattta ctacagatac aaatacacca ggaataccat
     1261 ctttattagt tgcggacagc atttcgacca atcgtcttgt ggtaaaacta tagtttttta
     1321 gaatattaca aaaggcacgt attgtttcgg tatggccatt ataaaatgcc ataaaaagtc
     1381 ctgatgtacc gttgggattt tttatccata ataattcatc tatatattcc tggtgagggg
     1441 ggagtatttt tagtatatct ccatacgcca taatggcctt tgtgtgccca ttttgaagtg
     1501 ccataaacaa cccatgcttt actttcaata attctttaat ttgttcacta ctaagcattt
     1561 tcttattaag cagacttccg aacatacgaa tactatctgc atgtccattc tgcaatgcaa
     1621 aaaataaccc aggtaaatca tggctgtctt tcgcagctaa tagatataat ctcttatcgg
     1681 aagttaacgg ggttttaaag actatttccc cataggcttt tattgtgtct acatgtccgt
     1741 tcataaaaga catgcataaa cccggcacat tgtttgcccc taccgcagca agcaatgtaa
     1801 gtattgtttc ttgactaaga ttgcacatat ttaatatctt accatatact tgaataatgt
     1861 ctgaacatcc gttttgaagt gctatataaa atgcaggagt accatcagag cttctcgtgg
     1921 ccagaaggtt caccagttta gattgagaaa tttcaccatt tttgtatttt tccatcagta
     1981 taaataatac ttctgtcaga ttctttgtta tcgcatgata tataactctg gaattgatca
     2041 attcatctgg taaaagtttc actgtgtttg ttagagggga attatgccta tcttcaaata
     2101 ttacctgccc gggttgctcc agaccataaa ttttccaata aaaatcatca aaaaaatcct
     2161 tagcggataa ttgactcagt tcgaaatggc tatcacaagt aaatacagta cgtttatgag
     2221 taacagttcg attaggatcg aaaaactgaa caacccattt ttcactttct tgtacgcgct
     2281 ttattttcaa ccctaacgcc atttggtggt ttaatgtgca tgctgataat gttctgacaa
     2341 agtctccatt ttttttcatg ttataaaaaa aattagtaat aactgttccc cactttttat
     2401 tttccacaaa ataaatataa gctggtgatt ttgaaaaatc ttccgttttt tccaaacata
     2461 cattttttgc aatagcatca ggggtgctaa gaagctgata gtttaccttc ccatttgttt
     2521 ccataaaatt catttcccaa taggaagcta ggtgacggca aactatttgt cttccatata
     2581 tatcttttgc cactccattt atatttacct tcacatttct cttttctgaa aaataaggtg
     2641 ctgttttatt cttaaactga attagttcac tggagtttga gaccctcata atagaacttg
     2701 ttattgttct attatctctg ttaacagaca tatctgttga taacgaattt tttgttgaaa
     2761 atatactaga tgaaaatgtt ttatttaacg gcatagaaga caaccataga gatgttataa
     2821 tactattaga ttgcagttac taatagtaaa agcctcctta caaggaggta gcttattcag
     2881 aatgctcgct ataagatagc gagctcgtca acgatacaga tgatctgatt atattgtgca
     2941 aaaggagaac aggaaaatag gcactggaaa agatgaaggc ctgatgggag cattaactaa
     3001 tgcaaaatcc gacaggttaa taacccgaaa agcaatcaaa cgactcaaac aggagatgca
     3061 cgctgaagtg gtggacaact gttcggattt cagatgacta gctcagcagt ttatgagtcg
     3121 aatgctctgt cacgggcagg aatcaggctg catacgctgt gccaaatcta atcgtagtta
     3181 ttctaaccag ttttcaagct acaaggtggt gtagctggca tcttctctac tctatcatca
     3241 aacgataaaa tggttcctct cgtaacattt gctcttgcct ttcacttcca tctcgatcgc
     3301 ttcgtagttc attacgatag ctgacatatt tccagtcttc aggtctaaca tctgacgccg
     3361 gatactcccc gggagcgatt ttgcttctta atagattaga ctcgagacac attataaatt
     3421 gtcgtatttt atgaagtaat aaagcactat attgcgagca ttcctgatct gattttacat
     3481 atagcgtaaa ctgagctcct atccccacac gagattgctg agaaactcga ttcatatcag
     3541 ttatcttcca tttatctata ggactgtcct ctgaaaataa taaaccagac aaaatttgga
     3601 atgccagagg aacttgctcc ctagctatac taatatgaaa cttgtcccca acaaaatccc
     3661 ccttactctg aggattttca cgacatgcat gaataaacac atctccacta tggttcacaa
     3721 tctggaatcc ttggtacatg acagaaaaaa attgacatat atcagagtaa gttggcacgt
     3781 tcgatgctac tttgaaatag tacgctgggt attgccgtat ttggttgtac aatatattga
     3841 agttattttt caatctttca ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat
     3901 tcaaagaatc taaatttagt tttagacagg gcttttttat gggcatagaa aacgtcctct
     3961 ataaaataga tatattatct tatattgatt gtttatcatc ttttataaaa cgatacatta
     4021 tatgtaatgt atgtaagcgt aagggtcttc
//
LOCUS       DBKEKI010000233         4019 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.236, whole genome shotgun sequence.
ACCESSION   DBKEKI010000233 DBKEKI010000000
VERSION     DBKEKI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.236"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(32..1486)
                     /gene="xylB"
                     /locus_tag="ACUQPX_004189"
     CDS             complement(32..1486)
                     /gene="xylB"
                     /locus_tag="ACUQPX_004189"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HHV9058647.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAMVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1558..2880)
                     /gene="xylA"
                     /locus_tag="ACUQPX_004190"
     CDS             complement(1558..2880)
                     /gene="xylA"
                     /locus_tag="ACUQPX_004190"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HHV9058648.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3246..>4019
                     /gene="xylF"
                     /locus_tag="ACUQPX_004191"
     CDS             3246..>4019
                     /gene="xylF"
                     /locus_tag="ACUQPX_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HHV9058649.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGT"
BASE COUNT         1055 a         1062 c          919 g          983 t
ORIGIN      
        1 atcccccacc cggtcaggca ggggataacg tttacgccat taatggcaga agttgctgat
       61 agaggcgacg gaacgtttca cgtcgtggct gataagcggc ataacgctgc gcatctggta
      121 gatgcgactg ttctaacggt agctgcggca acaactcaat gagcgatttc tctggattcg
      181 ccgcaatctg cgccagcctt gctgcgccca gtgctggccc cacatccccc cccgtacggt
      241 aatcgagctg ctgaccgctg atatccgcca gcatctgacg ccagtactca ctacgcgccc
      301 cgccaccaat caacgtaaca ctttgcggtt taatgccgca ggcatgcacg acatccatgc
      361 catctgccag cgcataaccc acgccttcca gcactgctcg cgccagttca ttggggccat
      421 gttgatgagt caaaccaaag aaaaccccct tcgcctgggg attattgtgt ggtgtacgct
      481 cgccggaaag ataaggcaga aaccaaactg gctcggcact ttcatcagcc tgttgagctg
      541 cagcgattaa agctgggaca ttgcacaggc cggttaattt cgcgacccaa tccagacacg
      601 atgctgcact cagcattaca gacattaaat gccaacgctg cggtagcgca tggcaaaagc
      661 tgtgtacagc gctttctggc ttgcttaaga acccttcgct gacagcaaaa tagacccccg
      721 acgtccccag cgataacatt gcctgattag catcaaccat tcccacacca actgcaccag
      781 ctgcattgtc gccaccgcct gcgacaactg gcaccatcgc cataccccac gctttcgcaa
      841 cttcaggtaa caaagcacca gtaatttcgc tgccttcgta taatgcgggc atctggtcac
      901 gagataagtc gcaagcctgc agcatgacgt cactccagtc acgctttgcg acatccagcc
      961 acatggtgcc agctgcgtca gacatatcgc tggcaaactc ccccgtcata cgcagacgca
     1021 agtaatcttt cggtaataat actttgtcga tttgacggaa tatctccggc tcatgccgct
     1081 gaacccatag caatttaggc gcagtaaatc cgggcatcat caggttgccg gtaatcactc
     1141 gtgattgcgg aactctcgct tccagcaaag tgcactcttg cgcacagcgc ccgtcgttcc
     1201 acaaaatggc agggcgcaat acccgttgtt gagcatccag taaggttgct ccatgcatct
     1261 ggccggcaat acccaatgct ttaacgtcct gcagagaatg ctgatcgccc agagctttca
     1321 ttgcgcgatc agttgcctgc caccactgtt ccgggtcttg ttccgaccaa agtggatgcg
     1381 ggcgcgaaac ggtcagcttt tccgtttgcg aagcaaccac ctcaccctgc tcgttgagca
     1441 aaataacttt tacgcccgag gtgccaagat ctatcccgat atacatatcg atcgttcctt
     1501 aaaaaaatgc ccggtatcgc taccgataac cgggccaacg gactgcacag ttagccgtta
     1561 tttgtcgaac agataatgat ttaccagatt ttccagttgc tcctggcgac cactctgatg
     1621 caccggagac aaattatgtt cctgagcata tttggctaaa tctgccagtg acatttggcc
     1681 tttcaggatt tgctggccca attcgctatt ccagccggaa taacgctgcg cgatgcgttt
     1741 atccagctcg ccatcttcaa tcatgcgcgc tgcaattttc agcgccagtg ccatcgtatc
     1801 catcgcgccg atatgaccgt aaaacagatc atatttatca gtactttgac gacgtacttt
     1861 ggcatcgaag ttcagaccac cggtggtgaa accgcctgct ttgagaattt catacatcac
     1921 cagcgcattc tcttccacac tgttcgggaa ctggtcggtg tcccagccca gttgcgcatc
     1981 gccacggttg gcgtcgacag aaccgaacag gccaagcgca atggcggtgg ctatttcatg
     2041 atggaaagag tgacctgcca gcgtcgcgtg gttagcttca atgttcagtt taatctcttt
     2101 ttccagacca aactgtttca ggaagccata gaccgtcgcg gcatcgtaat catattgatg
     2161 tttagtcggt tcttgcggtt tcggttcgat aagcaacgta ccctggaagc cgattttatg
     2221 tttatgctca accaccatct gcataaagcg gcccagttgt tcacgctcct gacgcaagtc
     2281 ggtatttaac agcgtttcgt aaccttcacg accaccccac aggacatagt tttcaccgcc
     2341 caatttatgg gttgcttcca tcgctgtaac aacttgcgtt gccgcccagc tgaagacttc
     2401 aggatctggg ttcgtcgccg cacccgcgcc atagcgaggg tttgtaaagc agttagcggt
     2461 tccccacagc agcttcacgc cgctctcttc ttgcttggct gccaggacat caaccatttg
     2521 cgcaaaatta ttgatgtact cttttaacga cgcgccctca ggggaaacat ccacatcgtg
     2581 gaagcaataa aatggcacat gtaacttgtg gaaaaactca aatgcgacat ctgctttacg
     2641 cttcgccaac gccagtgcct caccaggctg ctgccacgga cgattaaacg cccccacacc
     2701 aaacatgtcc gccccgttcc agcagaaggt gtgccagtag caggcggcaa aacgcaagtg
     2761 ctcttccata cgcttaccca acaccagttc gtcgggattg tagtgacgga atgctaacgg
     2821 gtttgaggat tttgagcctt cataacgaac gcgatcgagc tggtcaaaat aggcttgcat
     2881 attgaactcc ataatcaggt aataccgcgg gtgatggatg atgtcgtaat attgggcact
     2941 ccctttcagt tgctcaatta tgttatttca cactgctatt gagataattc acaagtgtgc
     3001 gctcgctcgc aaaataaaat gggatgatga aactgggtaa ttcctcgaag ataaaaatgc
     3061 aataagtaca attgcgcaac aaaagtaaga tctcggtcat aaatcaagaa ataaaccaaa
     3121 aatcgtaatc gaaagataaa aatctgtaat tgttttcccc tgtttagttg ctaaaaattg
     3181 gttacgttta tcgcggtgat tgttacttat taaagctgtc ctctaactac agaaggccct
     3241 acaccatgaa aataaagaac attctactca ccctttgcac ctcacttctg cttaccaacg
     3301 ttgcggcaca cgccaaagaa gtcaaaatag gtatggcgat tgatgatctc cgtcttgaac
     3361 gctggcaaaa agatcgagat atttttgtga aaaaggcaga atctctcggt gcgaaagtat
     3421 ttgtacagtc tgcaaatggc aatgaagaaa cacaaatgtc gcagattgaa aacatgatta
     3481 accggggtgt cgatgttctt gtcattattc cgtataacgg tcaggtatta agtaacgttg
     3541 taaaagaagc caaacaagaa ggtattaaag tattagctta cgaccgtatg attaacgatg
     3601 cggatatcga tttttatatt tctttcgata acgaaaaagt cggtgaactg caggcaaaag
     3661 ccttggtcga tattgttccg caaggtaatt acttcctgat gggcggctcg ccggtagata
     3721 acaacgccaa gctgttccgc gccggacaaa tgaaagtgtt aaaaccttac gttgattctg
     3781 gaaaaattaa agtcgttggt gaccaatggg ttgatggctg gttaccggaa aacgcattga
     3841 aaattatgga aaacgcgcta accgccaata ataacaaaat tgatgctgta gttgcctcaa
     3901 acgatgccac cgcaggtggg gcaattcagg cattaagcgc gcaaggttta tcagggaaag
     3961 tagcaatttc cggccaggat gcggatctcg caggtattaa acgtattgct gccggtacg
//
LOCUS       DBKEKI010000234         3969 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.237, whole genome shotgun sequence.
ACCESSION   DBKEKI010000234 DBKEKI010000000
VERSION     DBKEKI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.237"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..473
                     /locus_tag="ACUQPX_004192"
     CDS             <1..473
                     /locus_tag="ACUQPX_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019453456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9058650.1"
                     /translation="ANTAAEGGHGHGTVHFKGEIIDAPCSISPESADQTVDLGQVANS
                     ALKDKGTSSPVAFNIKLQNCDISTYKTVTATWSGTPDVNMPEAWGITGTASGAAIVLR
                     DASEKEITLGQDTAPTTLTADDTTIAMSAFLKGDGEAIVPGAFTGAADFVLSYQ"
     gene            549..1070
                     /locus_tag="ACUQPX_004193"
     CDS             549..1070
                     /locus_tag="ACUQPX_004193"
                     /inference="COORDINATES: protein motif:HMM:NF012635.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9058651.1"
                     /translation="MLRIVFLLLLLVSSTGRAVSHGHGHVQMQGTIIDTACAIATGDV
                     EQGIDFGALTASELINNGRGPSLPFTVHLVNCVLDGTDIRGLNHWKDVRITFVGDADS
                     ENHFALQGDGRGAALVIADSQGVEAKPGEPMPSTPITPGSMALHYQLWLTGGKTIRPG
                     HIHTTVRYFMEYD"
     gene            1070..3667
                     /locus_tag="ACUQPX_004194"
     CDS             1070..3667
                     /locus_tag="ACUQPX_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015378835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HHV9058652.1"
                     /translation="MFNQKSGNNGGLNNVRLNSLTWMLLLVLGGIPAAQSQDNMSTPA
                     NSNIPDEVVEQPNITEFNTDVMDVNDRNNIDVSHFSRAGYVMPGEYPLILVVNGTEIR
                     EVTVSFAPPEDDPQGSSPCLTPEMVKLFGLKDEATKKLIWKGNGDDRCLDEKSLPGMT
                     ATGDLSTGSLKISLPQAWLEYSAPDWDPPSRWDNGVADLLFDYNLNASKSHALSEGGS
                     SATSLSGSGTAGANFGPWRLRADWQASRYSDRGYTSQSMDWSRFYLYRALPKLGARLT
                     LGESDLDSSLFDSFRFIGGSLVTDDNMLPPNLQGYAPEVTGVARSNAKVVISQQGRVI
                     YESQVAAGPFRIQELSSSVSGTLDVRVEEQDGRVQHFQVDTASIPYLTRPGLVRYKAS
                     LGKPTDWKHHVQDVSFATGEFSWGMTNGWSLFGGLVAAKKYAALAVGIGRDLLSFGAV
                     SFDVTESKATLDTGEKKMGGSYRLSYSKRFEKIDGQITFAGYRFSEKDFMSMNDFLET
                     EYDAGSRSDTAWGRQKELYTITVSKAFPDWNMNLYGNYSHRTYWNRPDADRYSLSLSK
                     YMDVGRWKGISLSLTGYRSEAWGVKDDGVYASLSIPWSDGGSLSFNGQSTTAGTTQMV
                     GWYDRIDSNNNYSLQAGTSVGNKASASGYVSHNGDLTNGTLSASYYEGNNTSLGLSLQ
                     GGMTVTRYGAALHPGAMNGGTRLMIDSGDVAGVPLSHSGVLQRTNAFGVGVIGNLNSY
                     WRNSVGVDVNALDDDMEVSHPLTELTMTESAIGYRKIDVLEGRKLMAVVKFADGSSPA
                     FGATVWRGSHQTGVVDDNGSVWLAGVKPGVTMEVREDENPVCQVGIPERLPPENTDQS
                     LLLTCQQKS"
     gene            3781..>3969
                     /locus_tag="ACUQPX_004195"
     CDS             3781..>3969
                     /locus_tag="ACUQPX_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005184879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HHV9058653.1"
                     /translation="MNRNKSIKSQPFLKSLLVMALVSQSAFQVADAAVALDRTRVIFE
                     GGNKSMSLNIRNDNTKLPY"
BASE COUNT         1006 a          841 c         1109 g         1013 t
ORIGIN      
        1 ctgctaatac tgcggcagaa ggcggccatg gtcacggcac tgttcacttt aaaggtgaaa
       61 tcattgatgc gccatgctct atttctccgg aatctgcaga ccagacggtt gatcttggtc
      121 aggtcgctaa ctctgccctg aaagacaaag gtacgtcttc tccggtcgct ttcaacatta
      181 aactgcaaaa ctgcgacatc agcacttaca aaactgtaac tgcaacctgg agtggtacac
      241 ctgacgttaa catgccggag gcatggggta tcactggtac ggcgagcggt gcagctattg
      301 ttctgcgcga tgcgtccgaa aaagagatca ccctgggtca agacactgcc ccaactacgc
      361 tgaccgctga tgacaccact attgctatgt ctgcgttcct gaaaggcgac ggcgaagcga
      421 ttgtcccagg tgcgttcacc ggtgcggcag acttcgttct ttcctaccag taagtagccg
      481 cttaactgtg ctggctccct ggttagcagg gagccgttaa gaacgtgaaa tcagacggaa
      541 gtatggtgat gttgcgtatt gttttcctcc tgcttttgct ggtgagttcg acagggcgcg
      601 ctgtttcaca tggtcacggg catgtacaaa tgcaaggaac cattattgat acagcatgtg
      661 ccattgctac aggagatgtg gagcagggga tagatttcgg cgcacttacg gcaagtgaat
      721 taattaataa tggccgtggt ccttctttgc catttaccgt ccatttagtg aactgcgttc
      781 ttgatgggac agatattcga ggcctgaatc actggaaaga cgtacgtata acatttgtag
      841 gcgatgctga tagcgagaac cattttgctt tacagggaga tggacgtggc gcggctctgg
      901 tcattgctga tagccagggg gttgaggcga aacctggaga gcctatgcct tctacaccca
      961 ttacacctgg gagtatggca ttgcattatc aattatggct aaccggcggg aaaacaatcc
     1021 ggcctgggca tattcatacc accgttcgct acttcatgga gtacgactga tgtttaatca
     1081 gaaatccgga aataacggcg gattaaataa cgtcagattg aattcgttaa cgtggatgct
     1141 gttgctcgta ttgggtggta ttcctgctgc gcagtcacaa gataacatgt caacgccagc
     1201 gaattcgaat attccagatg aggtagtcga gcagccgaat ataaccgagt tcaacaccga
     1261 tgtgatggac gtaaacgatc gaaataatat cgacgttagc catttttccc gtgccgggta
     1321 cgttatgccc ggggaatatc cgctcatatt agttgtgaat ggaactgaaa ttcgcgaggt
     1381 cacggtttca tttgcaccgc cggaagatga cccacaaggc agctccccgt gtttaacacc
     1441 ggaaatggta aagctttttg gtcttaaaga tgaagcaacg aaaaaactaa tctggaaagg
     1501 taacggtgat gatcgttgtc tggatgaaaa gtcacttccc ggcatgaccg ctaccggtga
     1561 ccttagtacc ggttcactta agatttcttt accccaggcc tggctggaat atagcgcgcc
     1621 ggactgggat ccgccatccc gctgggataa cggagtggca gacttattat ttgattacaa
     1681 cctgaacgca tcgaaaagcc atgcgttatc tgaaggtggt tccagtgcga ccagcttaag
     1741 tggtagcgga acggcaggcg caaattttgg gccatggcgt ttacgtgccg actggcaagc
     1801 ctcgcgttat agcgacaggg gatacacgtc gcagagtatg gactggagtc gattttatct
     1861 ctaccgcgcg cttcctaagc ttggcgccag gttaactctg ggtgaatctg atcttgattc
     1921 atcactgttt gatagcttcc gctttatcgg cggtagtctg gtgactgatg acaatatgct
     1981 gccgcctaat cttcagggat atgctcctga agtgacgggg gtagccaggt caaatgcgaa
     2041 ggtcgtgata agccagcaag ggcgcgtgat atatgaatcg caggtggcag ctggtccttt
     2101 tcgtatccag gaattaagca gttccgtcag cggtaccctg gatgtccggg tggaggagca
     2161 agatggacgg gtacaacatt ttcaggttga tacggccagt attccctatc tgacgcgtcc
     2221 ggggttggtg agatacaaag cgtccttagg aaagcctacg gactggaaac accatgtaca
     2281 ggatgtgtct tttgcgacgg gagagttctc ttggggtatg acaaacggtt ggtcactatt
     2341 tggtggcctg gtcgcagcca aaaaatatgc ggctcttgca gtgggtattg gtcgcgattt
     2401 gctgtcattt ggtgcggttt cgtttgatgt caccgaatct aaggcgacgc ttgatactgg
     2461 cgaaaaaaaa atgggtggtt cataccgcct gagttattcc aaacgctttg agaaaataga
     2521 tggacagata acgttcgcgg gctatcgctt ttcagaaaaa gactttatgt caatgaacga
     2581 ttttctcgaa acagagtatg acgccggtag caggagtgat actgcctggg gaaggcagaa
     2641 agagctttac accatcacag tcagtaaagc ttttcctgac tggaatatga atctgtatgg
     2701 caattatagc caccggacct actggaatcg tcccgatgca gatcgctata gcctgtcttt
     2761 atcgaaatac atggacgttg ggcgttggaa ggggattagc ctgagcctga ctggctatcg
     2821 cagtgaggca tggggtgtga aggatgatgg tgtatatgcc agtctgagca ttccctggag
     2881 tgatggcggt tcattgagtt tcaacgggca aagcacaacg gctggcacaa cacagatggt
     2941 tggttggtac gacagaattg atagtaacaa taattacagt ctgcaagccg gtacaagtgt
     3001 tgggaataag gcctcggcga gtggatatgt gtcacacaat ggtgatctca cgaatggaac
     3061 gctgagtgcg agttattacg aaggaaacaa tacgtcgtta gggctgtctt tgcagggcgg
     3121 aatgacagta actcgttacg gagcggcgtt gcatcctggt gcgatgaacg gcggtacgcg
     3181 actgatgatt gattctggtg atgttgctgg tgtgccgtta agccatagtg gggttctgca
     3241 acgcacgaat gcctttggcg tcggcgtcat tggtaacctt aatagctact ggcgcaacag
     3301 tgttggggtc gatgttaatg ccctggacga tgatatggaa gtcagccatc cgttaacgga
     3361 attgaccatg actgaaagtg cgatcggtta tcgaaaaatc gatgttcttg aaggtcgaaa
     3421 attgatggcg gtagtgaagt ttgcggatgg tagtagtccg gcatttggcg ccaccgtctg
     3481 gagagggagc catcagaccg gcgtcgttga tgataacggt agcgtgtggt tagccggggt
     3541 taagccgggt gtcacgatgg aagtccgtga agatgaaaat ccggtatgtc aggtaggtat
     3601 tcctgagcga ttaccgccgg aaaataccga tcaatcgctg ttattaacat gccagcagaa
     3661 atcataactc attatgtgag tggggcactg tattggaaaa acagtgtgtg tagagattag
     3721 gagaaaagga cgcggccatg gacggtacat taatgttgat aattaaattc agagaatcaa
     3781 atgaacagaa ataaatcaat taagtcccag ccgttcctga aatctcttct ggtcatggct
     3841 ttagtcagtc agtccgcatt tcaggttgct gatgcggcag ttgctctgga tcgtactcgg
     3901 gtaatttttg aaggcggtaa taagagcatg agcttaaata ttcgtaacga taatacgaag
     3961 ctcccttat
//
LOCUS       DBKEKI010000235         3943 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.238, whole genome shotgun sequence.
ACCESSION   DBKEKI010000235 DBKEKI010000000
VERSION     DBKEKI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.238"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            433..1845
                     /gene="ospC1"
                     /locus_tag="ACUQPX_004196"
     CDS             433..1845
                     /gene="ospC1"
                     /locus_tag="ACUQPX_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HHV9058654.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(1949..2098)
                     /locus_tag="ACUQPX_004197"
                     /pseudo
     CDS             complement(1949..2098)
                     /locus_tag="ACUQPX_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2174..3871
                     /gene="ospD3"
                     /locus_tag="ACUQPX_004198"
     CDS             2174..3871
                     /gene="ospD3"
                     /locus_tag="ACUQPX_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HHV9058655.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFAENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1417 a          659 c          690 g         1177 t
ORIGIN      
        1 aagtacaata attatatatg gccaaactaa cctctattaa aaaaacatca gtttttcaca
       61 cttagttgag cttcatgaaa aaatcatcga gggtttcatt gcagaataca cattccacta
      121 actggaagca ctccataaga agaatcagag aaagtatcgt ttttttatag taaaattcat
      181 tgctgttcaa taaaattgat atagttatta aattatgaaa ataatttcac aaaaaagatt
      241 aacttgagtt cttaagcatc ggattttaga tattttgaaa tatctggcat cacataacat
      301 caaaagtata taataccctt atctaattac atgcagtata ttgtatatat actcaaaaat
      361 tgtaattcat accctgctat atcttgtagg gaaagatatt aattaaaact gttttcatat
      421 aaggctcatt ttatgaatat atcagaaaca ctgaactcag caaataccca atgcaatata
      481 gattctatgg ataacagatt acatacattg tttccaaaag tgacatcagt gcgaaacgct
      541 gcacaacaaa ctatgccaga tgaaaaaaat ttaaaagata gtgcaaatat tattaaagat
      601 ttctttagga aaactatagc agcacagagt tatagtagaa tgttctctca aggctctaac
      661 tttaaatctt taaatatagc aattgatgca ccatcagacg ctaaagcctc atttaaggct
      721 attgagcacc ttgacagatt atcgaagcat tatatatctg aaataaggga aaaacttcat
      781 cctctttctg cagaggaact caatttgctt tcgctaatta ttaattctga tttaatcttc
      841 agacatcaaa gtaattctga tttgtctgat aaaattttaa acattaagtc attcaataaa
      901 attcagtctg aaggaatatg cacaaaacga aacacatacg ctgatgatat aaaaaaaata
      961 gctaatcatg actttgtgtt ttttggcgtt gaaatctcta gccatcagaa aaaacacccc
     1021 ctgaatacaa aacatcacac tgttgatttt ggtgcaaatg cgtatatcat tgatcatgac
     1081 tctccatatg gatatatgac attaaccgat cactttgata atgctattcc acctgttttt
     1141 taccatgagc accaatcatt tttagataaa ttttcagagg ttaataaaga agttagtcga
     1201 tacgtacatg gaagtaaagg aattatagat gtaccaatat tcaatactaa agatatgaag
     1261 ttagggctcg gattatacct gattgacttt attagaaaaa gtgaagacca aagcttcaag
     1321 gagttttgct atggaaaaaa tcttgcccct gtggatctgg atagaatcat aaactttgtt
     1381 tttcagccag agtaccatat acctaggatg gtaagtacag aaaacttcaa aaaagttaag
     1441 attagagaaa tatccttaga ggaggctgtt acagcatcta attacgaaga aattaacaag
     1501 caggtcacta acaaaaaaat tgctctccag gctctttttc tttcgattac taatcaaaaa
     1561 gaggatgtcg ccttatatat attatctaat tttgagataa ctagacaaga tgttatttcc
     1621 ataaagcatg agttgtatga tattgagtat ctacttagcg ctcataattc aagctgtaaa
     1681 gtacttgagt attttatcaa taagggattg gttgatgtaa acacaaagtt caaaaaaact
     1741 aatagtgggg attgtatgtt ggataacgca ataaaatatg agaatgcaga aatgataaaa
     1801 ctattattga aatatggtgc aacatctgac aataaatata tttaatcaaa attgaatatc
     1861 gtttagcaaa aggcagtatt tgcacaaaat atcgatatat tgtttattgt cagtatggct
     1921 caatgtgata atagttggaa agtttgatgg gtttcgcccc gttgtagcgg tagtcgagct
     1981 ggaaggtctt caggcactgc ttacagcgat agagcagccc ccctgaactg gaatggccgt
     2041 tccggatacc cccctgagtt tcagagtaac ggggacaaac cacatcaatc tttgccatca
     2101 atcatccaaa gggcaaagag tacaacaaca ctaagtctgc gtcacaaccc atcaatgaaa
     2161 ggaatatata catatgccat cagtaaattt aatcccatca aggaaaatat gtttgcaaaa
     2221 tatgataaat aaagacaacg tctctgttga gacaatccag tctctattgc actcaaaaca
     2281 attgccatat ttttctgaca agaggagttt tttattaaat ctaaattgcc aagttaccga
     2341 tcactctgga agacttattg tctgtcgaca tttagcttcc tactggatag cacagtttaa
     2401 caaaagtagt ggtcacgtgg attatcatca ctttgctttt ccggatgaaa ttaaaaatta
     2461 tgtttcagtg agtgaagaag aaaaggctat taatgtgcct gctattattt attttgctga
     2521 aaacggttca tggggagata ttatttttta tattttcaat gaaatgattt ttcattccga
     2581 aaaaagcaga gcactagaaa taagtacatc aaatcacaat atggcattag gcttgaagat
     2641 taaagaaact aaaaatgggg gggattttgt cattcagctt tatgatccca accatacagc
     2701 aactcattta cgagcagagt ttaacaaatt taacttagct aaaataaaaa aactgactgt
     2761 agataatttt cttgatgaaa aacatcagaa atgttatggt cttatatccg acggtatgtc
     2821 tatatttgtg gacagacata ctccaacaag catgtcctcc ataatcagat ggcctaataa
     2881 tttacttcac cccaaagtta tttatcacgc gatgcgtatg ggattgactg agctaatcca
     2941 aaaagtaaca agagtcgtac aactatctga cctttcagac aatacgttag aattactttt
     3001 ggcagccaaa aatgacgatg gtttttcagg attgctttta gctttacaaa atgggcattc
     3061 agatacaatc ttagcatacg gagaactcct ggaaacttct ggacttaacc ttgataaaac
     3121 ggtagaacta ctaactgcgg aaggaatggg aggacgaata tcgggtttat cccaagcact
     3181 tcaaaatggg catgcagaaa ctatcaaaac atacggaagg cttctcaaga agagagcaat
     3241 aaatatcgaa tacaataagc tgaaaaattt gctgaccgct tattattatg atgaagtaca
     3301 cagacagata cccggactaa tgtttgctct tcaaaatgga catgcagatg ctatacgcgc
     3361 atacggtgag ctcattctta gcccccctct cctcaactca gaggatattg taaatttgct
     3421 ggcctcaagg agatatgaca atgttcccgg acttctgtta gcattgaata atggacaggc
     3481 tgatgcaatc ttagcttatg gtgatatctt gaatgaggca aaacttaact tggataaaaa
     3541 agcagagctg ttagaagcga aagattctaa tggtttatct ggattgtttg tagccttgca
     3601 taatggatgt gtagaaacaa ttattgctta tgggaaaata cttcacactg cagaccttac
     3661 tccacatcag gcatcaaaat tactggcagc agaaggccca aatggggtat ctggattaat
     3721 tatagctttt caaaatagga attttgaggc aataaaaact tatatgggaa taataaaaaa
     3781 tgaaaatatt acacctgaag aaatagcaga acacttggac aaaaaaaatg gaagtgattt
     3841 tctagaaatt atgaagaata taaaaagctg aatattatct cgtggtgata cactgaattt
     3901 gaggtaaccg gctcatttaa accgtctggt ctgttacctc cgg
//
LOCUS       DBKEKI010000236         3931 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.239, whole genome shotgun sequence.
ACCESSION   DBKEKI010000236 DBKEKI010000000
VERSION     DBKEKI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.239"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..820
                     /locus_tag="ACUQPX_004199"
     CDS             <1..820
                     /locus_tag="ACUQPX_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058656.1"
                     /translation="KNAPHKVVLPDFTGLAIRLDAQIRNQFHQLKEDEHFLRYRQLSE
                     RWYQAGSISDRNNRSKRFEKIMDDSLDFLLYSQDVMPNINPDDLQWHDYEKASSKGKM
                     YCVALLFHVIARAAYEPESVGKDPTLPEYCRWMKNWIEKTLGHDFLDRMMIYCALFAP
                     AYFPALQRLSGEKETRDVHEFLAEHVRTLAQKNSSEVNYRDQRQFTFGYTMFTKQQFE
                     FLDMMRDVHYRIDCIEQLLLDLIERKVVDFTDASVAGTWIEKQIQRLESNDVKQ"
     gene            1229..1642
                     /locus_tag="ACUQPX_004200"
     CDS             1229..1642
                     /locus_tag="ACUQPX_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HHV9058657.1"
                     /translation="MIFLLPYIFVRLALKPRELSKGNAGAAGLYAFFAEHIDPTCGAV
                     VCDQQFLADHLEVSTRTIRNWLKYLETERALVRIPVAGRVCAYALDPHEVWKGYDNAK
                     EYAAFVTKTLVNKDGDIKRRIMSMFSPEERPDDRE"
     gene            1754..2158
                     /locus_tag="ACUQPX_004201"
                     /pseudo
     CDS             1754..2158
                     /locus_tag="ACUQPX_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061586541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(2265..3101)
                     /locus_tag="ACUQPX_004202"
     CDS             complement(2265..3101)
                     /locus_tag="ACUQPX_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HHV9058658.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSVAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3101..3904)
                     /locus_tag="ACUQPX_004203"
                     /note="aph(3'')-Ib"
     CDS             complement(3101..3904)
                     /locus_tag="ACUQPX_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HHV9058659.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT          984 a         1000 c         1021 g          926 t
ORIGIN      
        1 caaaaatgcc cctcataagg ttgtgttacc tgattttact ggtctggcaa ttcggcttga
       61 tgcacaaatc aggaaccagt ttcatcaact gaaagaggat gagcacttcc ttcgatatcg
      121 gcagttgtcg gaaaggtggt atcaggcggg ctctattagt gacagaaaca accgcagtaa
      181 gcgttttgag aagatcatgg atgactcact tgattttttg ctttacagcc aagacgtcat
      241 gcctaacatc aatccagatg atctccaatg gcatgattac gaaaaggcta gcagtaaggg
      301 taaaatgtac tgtgtagcgc ttctgttcca tgtcattgca cgggctgcat atgaacccga
      361 atctgtgggt aaagatccga cacttccaga atattgccgg tggatgaaaa attggataga
      421 gaaaacatta ggtcatgatt ttttagatag aatgatgatc tattgtgcgc tgtttgcccc
      481 agcgtatttt cccgcgttac aacgtctgag cggagagaaa gaaacgcgtg atgtccatga
      541 attcctggct gagcatgttc gcactttagc tcaaaaaaat tcttcagagg taaattatag
      601 agatcaacgg caattcacgt ttggctacac catgttcacg aagcaacaat ttgaatttct
      661 ggacatgatg cgcgatgtcc attaccggat tgattgtatt gaacagctcc ttctggacct
      721 tatagaacgt aaggttgtgg atttcactga tgcatctgtt gcaggcacgt ggattgagaa
      781 gcaaattcag cggcttgaat caaacgacgt aaagcagtaa ccctctgaac tgtaaattgg
      841 gtagtcagat ttaattattg gcacctgttc ataggtgcca cctttctgaa agaatgtatc
      901 ctaatttttc gatatccaaa ctgaccccta ttttagatgt ttcatttatc acaccctaaa
      961 gctgaatatt tatcacaagc catttttacc gataaataac taaggcgatc aatgccaatg
     1021 gacttacaat aataaacaga ttcttttttg ctattcaggt tcgtaacacg gggtctgaag
     1081 tccaatggaa cgaaaacgta cgttagtgaa gtaactgtat gatatatcaa gacataatgg
     1141 acatagaaaa acgccatcaa aacggtgtct ttttaatcga aaattggcac ttaacggacg
     1201 gcattgtcta ctaatcgatt tttagacgat aattttcctc ttaccgtaca ttttcgtacg
     1261 gttggccctc aaaccccggg agctgtcaaa gggcaatgcg ggggccgctg gcctgtatgc
     1321 cttcttcgct gaacacatcg accctacctg tggcgctgtt gtctgcgatc agcagttcct
     1381 ggctgaccat ctggaggtta gcacccgcac gattcggaac tggctcaagt accttgagac
     1441 agaacgcgcc ttggtcagaa tcccagtcgc tgggcgggtt tgcgcctacg ctcttgaccc
     1501 ccacgaggtc tggaaagggt acgacaacgc caaagaatac gcggccttcg tgaccaagac
     1561 gctggtcaac aaggacggcg atattaagcg ccggatcatg tcgatgttta gcccagagga
     1621 acgcccagac gacagggagt gacgggcact ggctggcaat gtctagcaac ggcaggcatt
     1681 tcggctgagg gtaaaagaac tttccgctaa gcgatagact gtatgtaaac acagtattgc
     1741 aaggacgcgg aacatgcctc atgtggcggc caggacggcc agccgggatc gggatactgg
     1801 tcgttaccag agccaccgac ccgagcaaac ccttctctat cagatcgttg acgagtatta
     1861 cccggcattc gctgcgctta tggcagagca gggaaaggaa ttgccgggct atgtgcaacg
     1921 ggaatttgaa gaatttctcc aatgcgggcg gctggagcat ggctttctac gggttcgctg
     1981 cgagtcttgc cacgccgagc acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc
     2041 gagctgtggg gcgcggcgga tggccgaaag tgccgccttg ctggttgatg aagtactgcc
     2101 tgaacaaccc atgcgtcagt gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg
     2161 ggtcgtttgc gggaaggggc ggaatcctac gctaaggctt tggccagcga tattctccgg
     2221 tgagattgat gtgttcccag gggataggag aagtcgcttg atatctagta tgacgtctgt
     2281 cgcacctgct tgatcgcggc cgcgatagct agatcgcgtt gctcctcttc tccatccgcg
     2341 ttccaagcta cggaaaggca cccataagcg tacgcctggt cgagcaggcg acgcggatcg
     2401 acgtccagcg cacgagagaa tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa
     2461 aggtcgtctc tgtcagccgg atcgtagaac atattggcgg cgccaaagcc cacttcaccg
     2521 accagaccga cgggatctat caccagccag ccgcgactgg agaacatgat gttttcatga
     2581 tgcagatcgc catgtagccc acgcagttcc gaggcattgc tcatcatttg atcggctata
     2641 atcgccgcgt ggacgtagtc agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga
     2701 aacaaagctg caaagcgatc ccggatcggg agaagggcag aaggcagggg ttcctcagat
     2761 gcggcataca gcttcgccat tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc
     2821 tcggcaacga tgtgagagag cattcgctcc ccggcatatt cgagcaacat cagattgttc
     2881 tcacgaccga gcaaccggac tgctcccctc ccattgcgcc ataccagata gtcggccccg
     2941 cgcagttcat cagcaatgtc ttttataggt ttcaatccct tgacgattgc aggagtcccg
     3001 tctggcaatg aaactttcca aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc
     3061 gaaacgtgcc aatgagcagg aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt
     3121 ccaatcgcag atagaaggca aggcgttcgc ggtcgggggc ttcgatcccc aatacattga
     3181 ataggacagc gaaggcgcgc tctgcttcat ctggcgctgc ccagttctct tcggcgttag
     3241 caatcatgag tgccaaatcg gcatagcgat ctgctgttcc gagccgccca aggtcgatca
     3301 gacccgtgca ttgaagagtt ttagggtcca ccatgaagtt cggcatgcag ggatcaccat
     3361 ggcaaacaac catatcggtg cgctcttggt cgagccgcac cggtagctct cgttcgacac
     3421 gagccaaaag atcgagctgc ggcgtactct tgtcctcgtc cggtaagaag tcgggattga
     3481 cggcattgcg ggacaccaca tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa
     3541 acggacattg atcaaccgat aggctgtgaa cagcgccaag ttgctgcccc attgacggcc
     3601 acgctttgag caaatccgct ccagacagat cagccgccgg tactcccgga attgccgtta
     3661 tcaccaagca tgcaccctcc tgttcctcct gccagttgat gacctcgggg caagccacac
     3721 ctcgaccttt gagccaaatg aggcggtcac gctctccagc gagctcaccg cggcgggaag
     3781 caggtgcgat tttcgcgaag gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt
     3841 ctccgcctct gacaggcaac cagtcagaat gcgattcacc aaaaaaaata ttagttcgat
     3901 tcaatggagg ttccttcagt tttctgatga a
//
LOCUS       DBKEKI010000237         3886 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.240, whole genome shotgun sequence.
ACCESSION   DBKEKI010000237 DBKEKI010000000
VERSION     DBKEKI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.240"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..1514)
                     /locus_tag="ACUQPX_004204"
                     /pseudo
     CDS             complement(1..1514)
                     /locus_tag="ACUQPX_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            complement(1776..3366)
                     /locus_tag="ACUQPX_004205"
                     /pseudo
     CDS             complement(1776..3366)
                     /locus_tag="ACUQPX_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            3784..3886
                     /locus_tag="ACUQPX_004206"
                     /pseudo
     CDS             3784..3886
                     /locus_tag="ACUQPX_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
BASE COUNT         1206 a          714 c          639 g         1327 t
ORIGIN      
        1 taatttggca attatgcatg ctgaacattc ttccagcatg tttactgtca cgggcgttaa
       61 gacctaaaac tgtccccgtt tcacctatgc gttctctccc accatttccg ttatgtgcaa
      121 caacttgatt tatacctaac atttgtgaac atgctaatag tgcttcaagt ttaggtctgt
      181 aatctgtcga aacagaaaaa taacgattaa aggatcgaca agagatagcc cggctagttt
      241 taaacttttt gccattaatt atagcatgat gctttttatt aataatttcc gctaattcga
      301 ccggatcgag agcattggtc atttcatcaa tagatgacag ttatcggtcc tataatgtaa
      361 cgttttcgct gatcatcaaa agttatccca tgatgatttg ccattatttt gtatttgggg
      421 tcatagaaac atacaggata atcttttatc ccgtcgtagg tatcctcatc aagtggtttc
      481 atgcgcgtga agttttgcat atagttacca ttgcaattcg tttcatgatt tccagctaat
      541 acatgaacat tttcattgat actcattatg cgttttaaca aatcgatgat aaattgatcg
      601 ccacagatat tactaaatcg atcgccggtt tggtcgccaa gaaacgtaca tggcgtgact
      661 gcgtctttat taataaccaa ataaggctga agactttcta aaacattatc tcttctgttc
      721 agtatactga ttattgtatt aacattagtg tcaagttgct cattaaagtt ctcccttagc
      781 aatacgttga gaacttcatc ttctatatta agcaactggc taaggaaatt ttctccttca
      841 gccatgattc ccaaatgtcc tgttgcaaaa agcgcgtaca aaactgcacc gactgagcca
      901 tcggtatcgc caaaataggt cggcctggta cttgtgggat caacaggtga acaattggag
      961 aaataggttc tgtcagcgaa ctggggtttt ataattgttt cgaatttaat atcaaataca
     1021 ttgtattgcg atatttccca gttccagatg ggtttgtacg actcagtaac tgcaacatca
     1081 ttagcattat catttactgt catattatta agtaaataaa caatgctgca ccaattaata
     1141 tcggtattat caagagggca accgtaattt ttactattaa ttgtcacaat aaactgataa
     1201 cagttattcc tacgtagaat atgagcgcgc atgcccgata gcatactata tttttctgaa
     1261 tgattcgttt gtatggtaaa tgaaattttg tcatcttgaa tagattgttg gaaacgttct
     1321 atttgccaat catcaatttt ttccacaact tcaccgagca ttgtcctagc aatacttgat
     1381 atatcatcac ctataccatt tgcaatatac ccaaacagtt ctttagtgaa gttggtaaca
     1441 tgctcttgca ccatatcatt tgttgcgatt gtcgttgtac tgccattagt gcctgccaca
     1501 gttccaattt tcatctttta cctcatatta ttcaccgcga ggaatacata gttaacattt
     1561 tatcttatga catacacctc atattcgatg ggaaaataat tatattttgc acaaatcatt
     1621 cagccttaaa gcataatatg aaatttctta tctcatagca gagaaagtcc ctaaagattg
     1681 tagaggcgtc atcagtacat acctctacaa tcgattaaga aaacctatga aatactggca
     1741 aaattaaaaa gagataccac agcactcatg aagttttatt gaggttgaaa acaactaagc
     1801 cctgggacac cttttttatc accacgttcg ttaggtgcta tcttccagga atttagcccc
     1861 atggtataat tatcaatttt ttgatgctgg tctaccccta atccataatg gccaaatttc
     1921 tgtttagttt ttttgagcgt ctgtccacat ttgaagatat cttcaggttt tggtctgaaa
     1981 gcagtagtgt tattaaaata attttcatat acaggtgtgg gtgctatagc attgcttcta
     2041 attagatgga gaagatgatc atccatgtgt tgtttatgtt ttttattaaa tatatttgct
     2101 aattcctcag gatcaattgg gtctttcatc tgattaaatg gaacctgcaa agcaccgaga
     2161 caataacatt gattttcttc atttcttatt attccgtgat gactggttaa aactttagtt
     2221 tctgagttat aattacaaac atcgagcgtt ttaattaagt tatacgtatc accggcagag
     2281 agtttataat tttctagtcg tacagtatag ttaccattaa aattgatttc atgatttcca
     2341 gccagaacga tgacattttt attaatacgg ctatctttat taccctccat atttcgcata
     2401 gaatttagca aagtaaggat atatttatca ccaaaaatag tactaaatct atcgcctgta
     2461 tgatccccaa ggaagataca aggggttatc gcatcgtcct ttaaaaaaac atgttgatgc
     2521 aactcattca atatcatgtc tctcgtattt aataaattgt agagcaattt attattttta
     2581 tgtatgtatg cagatgaagc catgtcttcg tgttttaata attcacataa taaactccaa
     2641 cccttttccc tgatgcctat atgccctgaa acgagcaatg aataaagtac agcgcctact
     2701 cttccatctg tatcgccaaa ataagccgtg ttggcatttt gtgggttaat cacaccacat
     2761 tgtttataaa agtctatttt agaaaacaca gatttaacat agctatcaaa cttatcctta
     2821 ggataacgtc tctttagaga catcccaacg ccagtcagct ttaaaattac cgctaagaag
     2881 agtaaagaaa ttttcaggta tgatagaaag cccattaact ttataaatta tgtctgattt
     2941 atctccggag aaattaataa aataataacc atccttacac tcaatcttaa ctgtaatggg
     3001 agacgataat tttatcacat ggttattgtc tggtataatt gtaaagataa acgtgttagt
     3061 agtattatcg gcaaaactat tatattcttg aatatctata gatggctcac tattgtaaaa
     3121 ccatattttc tccatctgaa gactatgttc atttaccctc cagtgaccat gttgcaggaa
     3181 atagatgata tgcatgccaa aatcgttatc tatttgcgaa tcattatcca gtttatcaat
     3241 ttcaactatc tttgaacgat gtgttttatc aaccttgcta tggtgttgat gattattaac
     3301 tttatctgaa tgcttatctt tatggtttga agggatgtac ttaaatatat tgtcaattgc
     3361 tttcataaat aacccgcgat atttttgacc tgttagcaac aaataaatga tacatgtctg
     3421 aaagacatat ttacgacgat ataaaacaat aatacttata tgttcgctga ttttaagcac
     3481 cgagactatc cggtctttta ctaaataatg gaaaacaatc catccctgga agacaattgt
     3541 aaaaaaaaac aacagaataa gaattaatct taggataaat atttatttat catggccttt
     3601 tgaccgctaa aaaatggcac tcggcacagt aaaccccgct atagttatgc tatgcatttg
     3661 tctttaatga tatctcgtcg ttatattaac aggctgaaat tatcattaat gcacatttca
     3721 atattagcag ggatacctga ggagtttaaa gtgaacatat atatcgggta gcttttcaaa
     3781 ttaatccctt tgattatagg cttaatttgc atcgcgttag gtggctttgt gctggaaagt
     3841 tcagggcaac gtcgcgggtc atgtgctgat ttctctggcg gccata
//
LOCUS       DBKEKI010000238         3865 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.241, whole genome shotgun sequence.
ACCESSION   DBKEKI010000238 DBKEKI010000000
VERSION     DBKEKI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.241"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..512
                     /locus_tag="ACUQPX_004207"
     CDS             <1..512
                     /locus_tag="ACUQPX_004207"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HHV9058660.1"
                     /translation="SISYAGNMVGVALTTEGECGLDMELQRATRGFHSPHAPDNHTFS
                     SNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLNDDPRDLQLLPIAGRLKCAHVNH
                     VEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWKSLPDIHSRANNPTSRMMRFAQL
                     STVKSFSPN"
     gene            534..1100
                     /gene="yieF"
                     /locus_tag="ACUQPX_004208"
                     /gene_synonym="chrR"
     CDS             534..1100
                     /gene="yieF"
                     /locus_tag="ACUQPX_004208"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HHV9058661.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMDVIGGACCQYHLRQILVFLDAMVMNKPEFIGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1154..2491)
                     /gene="adeP"
                     /locus_tag="ACUQPX_004209"
     CDS             complement(1154..2491)
                     /gene="adeP"
                     /locus_tag="ACUQPX_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HHV9058662.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            2656..3321
                     /gene="yieH"
                     /locus_tag="ACUQPX_004210"
     CDS             2656..3321
                     /gene="yieH"
                     /locus_tag="ACUQPX_004210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HHV9058663.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            3388..3861
                     /locus_tag="ACUQPX_004211"
     CDS             3388..3861
                     /locus_tag="ACUQPX_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HHV9058664.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1030 a          966 c          972 g          897 t
ORIGIN      
        1 tttctatttc ctatgccggg aatatggttg gcgtggcgtt aacaaccgaa ggtgaatgtg
       61 gcctcgatat ggaactacag cgtgcgacgc gcgggtttca tagcccacac gcgcccgata
      121 accacacctt ttccagcaat gaatcgctat ggatcagtaa acaaaacgat cctaacgaag
      181 cgcgggcgca gctcatcacg ctgcgccgaa gcgtgctaaa actaaccggt gatgttttga
      241 atgacgatcc gcgcgatctg cagctgctgc ccattgccgg acgcctgaaa tgtgctcatg
      301 taaatcatgt agaagcgtta tgcgacgcgg aggacgtgct ggtgtggtcc gtggcggtca
      361 cgcccgcgat tgaaaagctc agtgtctggg agttagatgg caaacacagc tggaaaagcc
      421 tgccggatat tcacagccgc gccaacaatc ctaccagccg gatgatgcgt tttgcccagc
      481 tctctaccgt gaagtctttt tcgccaaatt gatagacaac cacaggagtc attatgtctg
      541 aaaaattgca ggtggttacg ttactgggga gcctgcgcaa aggctcattt aatggcatgg
      601 ttgcacgtac cctgccgaaa attgctccgg cgagcatgga agtcaatgcg ttaccttcca
      661 ttgccgacat tcccctgtat gacgctgacg tacagcagga agaaggtttt ccagcaacgg
      721 ttgaagctct ggcggaacag atccgtcagg ctgacggtgt ggtgatcgtc acgccggaat
      781 ataactactc ggtaccgggt gggctgaaaa atgccatcga ctggctttcc cgcctgccgg
      841 atcaaccgct ggccggtaaa ccggtgttga ttcagaccag ctcaatggac gtgattggcg
      901 gcgcgtgctg tcagtatcac ctgcgccaga ttctggtttt cctcgatgca atggtgatga
      961 acaagccgga atttataggc ggcgtgattc agaacaaagt tgatccgcaa accggagaag
     1021 tgattgatca gggtacgctg gaccacctga ccgggcaatt gaccgcattt ggtgagttta
     1081 ttcagcgagt taagatctaa ataaaaaacc cgccagtaat catgcatggc gggtttttaa
     1141 tgcgctatcg attttaatga gcgtcgataa atacaatctt caggataaac agcagcgcaa
     1201 cgatgattac gcacgggcta aggtcacgca gacgcccggt accaatcttc atcacgcagt
     1261 aggagataaa gcccagcgca ataccttcgg taatcgaaaa gctgaacggc atcatcacgg
     1321 cggtaataaa cgccggaaca gattcagtaa gatcctgcca gttcacgcga gcaagacttg
     1381 aggtcatcaa cacgccaacg tagatcaacg cgccagctgc agcgtagcct ggcaccattc
     1441 ccgccagcgg cgacagaaag ataaccagca ggaacagcag accaacaacc actgccgtca
     1501 gaccggtacg accgccaacc gatacgccgg aagaggactc aatataagcc gtaacggaag
     1561 aagtaccgat aaacgaaccg gtcacggaag agatactgtc gacatacagc gcctgcttca
     1621 tgcgcgggaa tttccccttc tcatccgcca gacctgcttt atcggtcacg ccaatcagcg
     1681 taccggagga gtcaaacaag ttgaccaaca tgaaagagaa aatcacccct gccagcccga
     1741 ggttaaacga cccggctaaa tctacatgac ccacaactgt cattacgctc ggcggtgcag
     1801 aaacgatgcc attgtagtgc acatcaccca gcatccagcc cagcagcgtc gtcaccacga
     1861 tagaaaccag caccgctgcg tgaatgttgc gcgaggccag aatagcaatg atgaaaaagc
     1921 cgaggatacc cagaagtacg ctgtgagaag tcagattacc gatgctcacc agcgtttccg
     1981 agttagcgac aatcacacct gcgtttttca gccccatcat gccgataaac aggccgatac
     2041 cgctggtaat acccacacgc agacttaccg gaatgttagc aatcatccag taacgaacgc
     2101 ggaaaatggt caaaagaagc agtccgacag cgccccagaa gattgcgccc ataccgatct
     2161 gccacggcag ccccattgcc tggacaacca caaaagcgaa aaaggcgtta agccccatcg
     2221 caggagccag cgccaccgga aggtttgcaa acaatcccat cataatgcta ccgaaagcag
     2281 caatcagaca ggttgttacg aagacggcac tggtgtccat gccagcaacg cccagaattt
     2341 gtgggttaac aaatacgatg tagaccatcg tcaggaaggt ggtgaacccg gcaatcactt
     2401 cagttcgtgc cgtagtacca tgttcgcgca gtttaaaaac gcgttccagc atcctctgac
     2461 cagaagtctg ggtggtatgt tgatgactca ttatctattt ccgaacaagg agggaaaatc
     2521 cgtcgctatc gtataccaaa atgcgacaat aggtgcgttt gtgagagact tttttattgg
     2581 atttacttat acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga
     2641 ttaaaaagga aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg
     2701 gtcgacagtg aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt
     2761 acgctcgatc ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc
     2821 gatattgttt cccttgaaca tggtgttacg ttagcgaaaa cagaagctga acacgtttac
     2881 cgtgcagaag tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctggagcg
     2941 ctcctgtcag cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg
     3001 cagcattcta tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc
     3061 tacgatattc agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg
     3121 aatgtaaatg tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt
     3181 atcgacgcag gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt
     3241 cacccgaaag tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt
     3301 ggttgggata ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg
     3361 ctcaaaggta agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga
     3421 ctttattttg cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc
     3481 ttcgcaaaag aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta
     3541 ttgtcgatta ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg
     3601 ccggaaaaga tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc
     3661 gttatcaccg agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag
     3721 ttgtttgaaa acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg
     3781 gcaggtgtgg tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct
     3841 gaaacagact ctttaagtta agcgg
//
LOCUS       DBKEKI010000239         3859 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.242, whole genome shotgun sequence.
ACCESSION   DBKEKI010000239 DBKEKI010000000
VERSION     DBKEKI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.242"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            136..990
                     /gene="gatY"
                     /locus_tag="ACUQPX_004212"
     CDS             136..990
                     /gene="gatY"
                     /locus_tag="ACUQPX_004212"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HHV9058665.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            1019..2281
                     /gene="gatZ"
                     /locus_tag="ACUQPX_004213"
     CDS             1019..2281
                     /gene="gatZ"
                     /locus_tag="ACUQPX_004213"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HHV9058666.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMDKIYDVLRAYRYGCAE"
     gene            2291..2743
                     /gene="gatA"
                     /locus_tag="ACUQPX_004214"
     CDS             2291..2743
                     /gene="gatA"
                     /locus_tag="ACUQPX_004214"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HHV9058667.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2774..3058
                     /gene="gatB"
                     /locus_tag="ACUQPX_004215"
     CDS             2774..3058
                     /gene="gatB"
                     /locus_tag="ACUQPX_004215"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HHV9058668.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            3062..>3859
                     /locus_tag="ACUQPX_004216"
     CDS             3062..>3859
                     /locus_tag="ACUQPX_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HHV9058669.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKP"
BASE COUNT          970 a          849 c         1017 g         1023 t
ORIGIN      
        1 atcgggaggc cgtttttttg cctttcactc ctcgaacaat tttcatattg tcgtttttgt
       61 gatcgttatc tcgacattta aaagcaaata atttcattat attttgaaat cgaaagcaaa
      121 cgacaagata tgaaaatgta cgtggtatcg acaaagcaga tgctgaacaa cgcacagcgc
      181 ggcggttatg cggttccggc attcaatatt cacaatctcg aaacgatgca agtggtggtg
      241 gaaaccgctg ccaacctgca tgcgccggtc atcatcgccg gaacgcctgg cacatttact
      301 catgctggta cagaaaatct gttggcgctg gtcagcgcga tggcgaagca atatcaccat
      361 ccactggcaa ttcatctcga ccatcacacg aaatttgacg atatcgctca gaaggttcgt
      421 tctggcgtgc gctcagtcat gattgacgcc tcgcatttgc cttttgcgca aaatatttca
      481 cgggtcaaag aggtggtgga tttttgccat cgctttgatg tcagcgtcga ggcggagctg
      541 gggcaacttg gcggccagga agatgatgtg caagtcaatg aagccgatgc gttgtacacc
      601 aaccccgcac aggcgcgtga atttgccgag gcaaccggga ttgattccct ggcggtcgcc
      661 atcggcacgg ctcacggtat gtatgccagc gcaccggtgc ttgatttttc tcgactggag
      721 aacattcgcc agtgggtgaa cttaccgctg gtgctgcatg gcgcgtcagg attatcgact
      781 aaggatattc agcaaaccat caaactgggg atatgcaaaa tcaacgttgc aacggagctg
      841 aaaaatgcct tctcgcagtc gttaaaaaat tacctgaccg agcaccccga agcgaccgat
      901 ccccgggatt atttgcagtc ggctaaatcc gcaatgcgcg atgtggtgag caaagtgatt
      961 gccgattgtg gctgcgaggg cagggcataa cgcacctgcc atttaacaag gaaaaaacat
     1021 gaaaacgtta attgcccggc ataaagctgg tgaacatatc ggcatatgtt cagtctgttc
     1081 tgcccatccg ttggttatcg aagcggcgct ggcatttgat cgcaacagca cgcgcaaagt
     1141 gctgattgaa gcaacgtcaa accaggtcaa tcaatttggc ggttataccg gaatgacacc
     1201 ggcagacttt cgcgaatttg tttttacgat tgccgataaa gttgggtttg cacgcgaacg
     1261 cattattctc ggcggcgatc atctggggcc aaactgctgg cagcaagaaa atgcggatgc
     1321 ggcgatggaa aaatccgtcg agctggtaaa agcatatgtt cgtgctggct tcagtaaaat
     1381 tcatcttgat gcgtcaatgt cctgcgcggg ggatcccata ccgttagcac cagaaacggt
     1441 tgcggaacga gctgctgtgc tttgctttgc tgcggaaagt gtggcgacag attgccagcg
     1501 tgagcaactg agctatgtca ttggcactga agttccggtt ccgggcggtg aggccagcgc
     1561 cattcagtca gtacacatca cccatgttga agatgccgcc aatactttac gtacgcatca
     1621 aaaggccttt attgcccgtg ggctgacaga ggcgttaaca cgtgtgattg ccatcgtggt
     1681 gcagccgggt gtggaatttg atcacagcaa tattatccat tatcagccgc aggaagcgca
     1741 ggcgctggcg caatggatag aaaacacccg aatggtttat gaagcacatt ctaccgatta
     1801 ccagacccgg acggcttatt gggaattagt ccgcgatcac tttgcaatat tgaaagtcgg
     1861 tcccgcatta acctttgctt tacgcgaggc gatatttgca ctggcacaaa ttgagcagga
     1921 acttatcgcc cccgaaaatc gcagcggttg cctggcggta attgaagaag tgatgctcga
     1981 cgaaccgcaa tactggaaaa aatattatcg cacgggtttt aacgattcat tactggatat
     2041 tcgttacagc ctgtcggatc gtattcgtta ttactggccg catagtcgga ttaaaaatag
     2101 cgttgaaacg atgatggtga atctgcaagg cgtggacatc ccactgggca tgattagtca
     2161 gtatcttccc aaacaatttg aacgcattca gtccggggaa ttatcagcaa tgccgcatca
     2221 gcttattatg gataaaattt atgatgtttt gcgcgcctat cgctacggct gtgcggaata
     2281 aggacggtat atgactaacc tgtttgttcg tagcggaatt tcttttgtcg atcgtagcga
     2341 agttttaacc tatatcggta atgagatgct cgcgaaaggt gtggttcatg atacctggcc
     2401 acaggcatta attgccagag aagcagaatt ccctaccggg ataatgcttg agcagcacgc
     2461 cattgcaata ccgcattgtg aggcgattca tgctaagtcg tcagccattt atctgttaag
     2521 gccaacaaat aaagttcatt ttcagcaagc ggatgatgat aacgacgtgg cggtatcgtt
     2581 ggttattgcg ttgattgtgg aaaatccgca gcagcaattg aaacttttac gctgtttatt
     2641 tggcaagtta caacagcccg atatcgtcga gacactaatt actcttcctg aaactcagtt
     2701 aaaggaatac ttcacaaagt atgttttaga ttcagacgaa taaatccctc tgtaacaata
     2761 aaaaggacta tttatgaaac gcaagattat tgtcgcttgc ggaggcgcgg ttgcgacctc
     2821 tacgatggcg gcggaagaaa ttaaagagtt gtgtcagagt cataatattc ctgttgaatt
     2881 aatccagtgt cgggttaatg aaatagaaac ctatatggat ggtgtgcatt tgatatgtac
     2941 cactgccagg gtggatcgta gttttggcga tattccgtta gttcacggca tgccttttgt
     3001 ttctggtgtc ggtatcgaag cattacaaaa taaaattctg actatcttac aggggtgacc
     3061 tatgttttca gaagtcatgc gttatattct cgacctcggc cctacagtga tgctgccggt
     3121 tgtcatcatt attttttcta aaatattagg catgaaggca ggcgattgct ttaaagcggg
     3181 tctgcatatc gggattggct ttgttggcat tggccttgtg attggcttaa tgctggattc
     3241 cattggtccg gcggcgaaag cgatggcgga aaatttcgac ctgaatctgc atgtggtcga
     3301 tgtcggctgg ccgggctctt caccaatgac ctgggcgtcg caaattgcgc tggtggcgat
     3361 tccgattgcg atattggtta acgtggcgat gttactgacc cgtatgacgc gggtggtaaa
     3421 tgttgatatc tggaatatct ggcatatgac cttcaccggc gcgttgctgc acctggcaac
     3481 cggttcatgg atgataggga tggcgggtgt ggtaattcac gcggcgtttg tttataagct
     3541 aggcgactgg tttgcccgcg atacccgaaa tttctttgag ctggaaggta ttgctattcc
     3601 gcacggtacg tcggcgtata tggggccgat tgcggtgctg gtcgatgcta tcatcgagaa
     3661 aatcccaggc gttaaccgaa ttaaatttag cgccgacgat attcagcgca aatttggccc
     3721 ttttggcgaa cctgtcaccg tgggttttgt gatggggctg attatcggca tcctcgcggg
     3781 ttacgatgtc aaaggtgtat tgcagctggc ggtaaaaacg gcggcagtta tgctgctgat
     3841 gccacggatg attaaaccc
//
LOCUS       DBKEKI010000240         3564 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.243, whole genome shotgun sequence.
ACCESSION   DBKEKI010000240 DBKEKI010000000
VERSION     DBKEKI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.243"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..592
                     /locus_tag="ACUQPX_004217"
     CDS             <1..592
                     /locus_tag="ACUQPX_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0043711 - pilus organization [Evidence
                     IEA];
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis chaperone"
                     /protein_id="HHV9058670.1"
                     /translation="LPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQE
                     KIAQLPNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYE
                     KVEIIRRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPS
                     AFQVGYINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            594..1658
                     /locus_tag="ACUQPX_004218"
     CDS             594..1658
                     /locus_tag="ACUQPX_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9058671.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(1701..1901)
                     /gene="glgS"
                     /locus_tag="ACUQPX_004219"
     CDS             complement(1701..1901)
                     /gene="glgS"
                     /locus_tag="ACUQPX_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HHV9058672.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            2170..2799
                     /locus_tag="ACUQPX_004220"
     CDS             2170..2799
                     /locus_tag="ACUQPX_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HHV9058673.1"
                     /translation="MNLFADHNTPYLFAISFVLLIGLLEIFALICSHMLSGTLDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLNGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLQEPEEGKIFTKGDKVLIICRLSATRYLVENNPWPQIL"
     gene            2826..>3564
                     /locus_tag="ACUQPX_004221"
     CDS             2826..>3564
                     /locus_tag="ACUQPX_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0016600 - flotillin complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HHV9058674.1"
                     /translation="MDDIVNSVPSWTFTAIIAVCVLFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIE"
BASE COUNT         1070 a          738 c          728 g         1028 t
ORIGIN      
        1 tctaccctat ctggcgcaaa catggataga agatacgaaa ggcaataaat cacgtgactt
       61 tattgtcacg gttcccccta tggtacgttt aaatcccagt gagcaaattc aaataagaat
      121 gataagtcag gaaaaaattg ctcaactgcc taatgacaga gaaacgctct tctattttaa
      181 cgtgcgagaa ataccgccaa aaacagataa aaaaaatgtg atgcaagtca ctatgcagca
      241 cgcattgaaa ctattctggc gaccaaaagc tattgaatta gaagatgatg gggtaatgtc
      301 ttacgaaaaa gttgaaatca tccgtagaaa tgatggttcg atccgcttta acaataagat
      361 gccttatcac gttacgctgg gttatatcgg aacaaacgga atcacaatgt tgccacaaac
      421 gcaaagttta atggtaaccc cttatagcca tgcagatacg cagtttaaaa atgttccgtc
      481 agcttttcag gttggttata tcaatgattt tggtggatta agtttttatg aaataaactg
      541 cccgacggtt aataactctt gtaatgtttc cgtagccaaa cgagataaat aatatgcgct
      601 acttattaat tgtaattata tttattatag gttttagtgc attacctgta tgggcaatgg
      661 actgctatgc tgaacatgaa gagggaaata ccgtagtcat tggttacgta ccaagaattt
      721 ctatccccag taatggtaaa aaaggtgata agatctggca aagcagtgag tattttatga
      781 atgtcttctg taataatgca ttacctgccc catcacctgg agaagaatac ccatcagcct
      841 ggactaatat aatgatgttt ttaccaggcg gtcaggactt ttataatcag aactcctata
      901 tttttggcgt aacatacaat ggagttgatt atgattcgac tgcccctaat gctatcgcag
      961 caccagaatg cattgatata aaaggagcgg gtacctttaa taatcactat aaaaaccccg
     1021 ctgtctgtag tggaggtcct gagcctcaat tatcagtaac ctttccagct cgcgtacaac
     1081 tctatattaa attagcaaaa aatgccaata gagttaataa aaacctggtg ttgcctgatg
     1141 aatatatcgc tctagagttt aaaggtatga gtggcgcagg agctatagaa gtagacaaaa
     1201 atttgacctt caggattcgt ggattaaata atattcatgt ccttgactgc tttgttaatg
     1261 tcgcactgga accagctgat ggcgttgttg actttggtaa aataaattcc cgaacaatta
     1321 aaaataccac cgttagtgag acgtttagtg tagtcatgac aaaagatcct ggtgcggcct
     1381 gtactgagca attcaatatt ttagggagct ttttcactac ggatattttg agtgattata
     1441 gccatctgga tatgggtaat ggactgctat tgaagatatt tcataaagat ggaacagcaa
     1501 cggaatttaa ccgcttctca caatttgctt ctttttcatc gtctagtgca ccttcggtca
     1561 ccgcaccatt cagggcagaa cttagtgcga acccggcaga gactgttgtt gagggaccgt
     1621 ttagtaaaga cgtaatcctc aaaatcacct ataactagca tctaatacaa acactaaaac
     1681 gggccatcag gcccgttata tcagtgctct aactccagct ctcttgcctg catcatctgt
     1741 tggcaatacc aggcataacg tgcgcaaaac caggttctat gttcttcatc catgttacca
     1801 gtaacggcca gaatatcgac cgggcgacct tctgcgtgca ttctggcaaa actacgcgcc
     1861 aggaaatcga aattattcaa agaattaaga ctatgatcca taagcacatc ctccggttat
     1921 cgcatacctt ttaaggccat atgctttttc tgatttcagt attccccaat cagtgtagtg
     1981 cctttaaaca taacgtgcgt aaatatattg tccccgatca cactttttag tgaaaaataa
     2041 aaaaccatat taaaatactt tctcttccag aacttctcta acatgtgtta ttattaacaa
     2101 agacaaatgt aagcagggaa actattccaa ggtaaatatt gcgtcactaa atggacattg
     2161 gagtgacata tgaatttatt cgccgaccat aacacccctt atcttttcgc tatttccttt
     2221 gtactgttga tcggtctttt agaaattttt gcgcttatct gtagccacat gctctccggt
     2281 actcttgatg cacatcttga ccattatgat tccataacca caggtcatat tagccaggca
     2341 cttcattatc tcaatatcgg aagattgcca gctctcgtcg ttctctgctt actcgctggt
     2401 ttttttggtc ttaatggcat cctgttgcaa catgcctgca tcatggtctg gcaatcacca
     2461 ctgtcgaacc tgttcgtggt tcctgtcagt ttactgttta cgattattgc ggtgcattat
     2521 accggtaaaa tagttgcgcc ctggattcca cgcgaccata gttcagctat tacagaagaa
     2581 gaatatattg gtagcatggc gttaattacc ggtcatcaag ccacttcagg taatccctgc
     2641 gaaggaaaac tcaccgatca atttggccaa atacattatt tactacagga gcctgaagaa
     2701 ggaaaaattt tcactaaagg agacaaagtg ttaattattt gtcgactttc tgcaactcga
     2761 tatttggtgg aaaacaatcc ctggccccaa atattgtaat ttattaatca aaaggaaatt
     2821 taataatgga tgatatcgtt aattctgtgc cttcctggac gtttaccgca attattgccg
     2881 tatgcgttct gtttattatt ggaattattt tcgccaggct ctatcgtcgc gcttcggcag
     2941 agcaagcatt tgttcgtact ggtttaggtg ggcaaaaagt ggtgatgagc ggtggcgcaa
     3001 tcgtgatgcc gatctttcat gaaataatcc ccatcaatat gaatactttg aagctggaag
     3061 tcagccgctc aaccattgat agcctgatta cgaaagatcg tatgcgtgtc gatgtagtag
     3121 tcgctttctt tgtacgggta aaaccgtctg tagaaggcat cgccactgca gcgcagacgt
     3181 tggggcaacg cacgctatca ccagaagact tacgtatgtt ggttgaagat aaatttgtcg
     3241 atgccctccg tgcaacagct gcacaaatga ccatgcatga gttacaggat acccgcgaga
     3301 acttcgtgca gggagtacaa aatactgtcg cagaagacct gtcgaaaaac ggtcttgagc
     3361 tggaaagcgt ttcacttacc aactttaacc agacctcgaa agaacatttc aatccgaaca
     3421 atgcctttga cgccgaaggt ttaaccaaac tgactcagga gacggagcgc cgtcgtcgcg
     3481 aacgtaacga agttgaacag gatgtagaag ttgcggtgcg tgagaaaaac cgtgatgcac
     3541 tttcgcgcaa gttggagatt gaac
//
LOCUS       DBKEKI010000241         3555 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.244, whole genome shotgun sequence.
ACCESSION   DBKEKI010000241 DBKEKI010000000
VERSION     DBKEKI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.244"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..226
                     /locus_tag="ACUQPX_004222"
     CDS             <1..226
                     /locus_tag="ACUQPX_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HHV9058675.1"
                     /translation="EKEQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADYPHQ
                     LLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            236..1777
                     /locus_tag="ACUQPX_004223"
     CDS             236..1777
                     /locus_tag="ACUQPX_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HHV9058676.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGSGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRWVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGLHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            1774..>3555
                     /locus_tag="ACUQPX_004224"
     CDS             1774..>3555
                     /locus_tag="ACUQPX_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HHV9058677.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSKLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQHGRRVFMDFNRNPEAVPGDLPFTLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNSGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVIAVRLARFIGCTQKRSVEGDVAQLVASS
                     LNIVREVIAQALVNENGMALVTVKEKIQARMSDYAGFICHADKVRHATRDALLLSEFV
                     QQHGLTINHAGEITELFMWRHMALTSAA"
BASE COUNT          842 a          899 c         1001 g          813 t
ORIGIN      
        1 agaaaaagaa caggtggaaa gctggatggc gaaccagtta gcgcagctgg aaaaatgcgg
       61 tccggtggca ctggcgcata ttaagcaact gattctggca ctggaaaata aacatgccga
      121 ttacccgcac cagctactgg ccggattaat gtctgccact caggattgcc agcaggcgac
      181 gcgcgccttt gccgaaaaaa gtagcgtgag cttccataat caataaggaa attgtatgcg
      241 taagataaca accgccgagg cactggcggc gcaaattcag gacggtgcga ctattgctat
      301 tagcggtaac ggcggcggta tggtagaagc cgaccatatt ctggctgcta ttgaagcgcg
      361 tttcctgcaa accggacacc ctcgcgacct gacgctaatc cattcgctgg gtattggcga
      421 tcgcgactgc aaaggcacta accgttttgc gcacgcagag atgctcaaac gcattattgc
      481 cgggcacttt acctggtcgc ccaaaatgca ggcgctggta aaaaataaca cgattgaagc
      541 ctactgcttt cctggtggcg tgattcaggc actgctacgg gagatcggct ccggacgtcc
      601 ggggcttttt acccacgttg ggctgggatc gtttgttgat ccacgcaatg gcggcggtaa
      661 gtcgaatgaa tgcactaccg acgacctggt agaactgatt gaaatcgacg gtgaaaccaa
      721 acttcgttat cgccctttca aagtggatta cgcgattttg cgtggcactt atgccgatcc
      781 tcgaggcaac gtcagccttg aagaagaagc gattgatatg gatagctatt ccatggcgct
      841 ggcagcacac aacagcggcg gcaaagtgtt cgtacaggta cgcgatgtgc tggaagctgg
      901 cgcgattgaa ccacgctggg tcaaattacc gggcattctg gttgatggca tcgttgaaca
      961 ccgcgaacaa ccacaaacct atcttggcgg ttacgacctg accatcagcg gccaacatcg
     1021 ccgtctgagt tctaacgacg ccattgaact ggttagtcat ccggtacgtc gcctgattgc
     1081 ccgtcgggcg gcacgcgaac tggtagctgg cgcttcaacc aactttggct ttggtattcc
     1141 tgggggtatt ccaggcgtag cgctgcgcga aggcgtgcct taccaaagtt tatggctgag
     1201 cgtcgagcaa ggcctgcata acggtatgat gctggatgat gcgtttttcg gctgcgcccg
     1261 caacgccgat gccattattc catcactcga tcaatttgag ttctacagtg gcggagggat
     1321 cgatatcacc ttcctcggca tgggagagat ggatcagtac ggtaacgtca acgtatccca
     1381 tctcaatggc aatctcattg gtcccggcgg ttttctcgaa attgcgcaaa acgcccgtaa
     1441 agtggtgttc tgcggcacgt tcgacgccaa aggtagcaag attgatgtaa cgccagatgg
     1501 cttgcatatc acccagtcag gtcaaatccc taaactggtt acccaggtgg aaaaaatcac
     1561 ttttagcgcc gcctacgcac agcaaagtgg tcaggaagtg ttgtatatca ctgaacgcgc
     1621 agtattccag ttaacggcag aaggcgttga attaattgaa atcgcgccgg gtgtggagat
     1681 tgagcgcgac attctgccgt atatggcttt ccgtccaatt atcaagcacc cacgcctgat
     1741 ggaaagtagc ctgtttacgc cgatggagga tgcatgagtc agcttgatga caccatcctt
     1801 gatgcgctga cgcacgttac tttcccaaag ggttttgcac aggcagagcc cgcatgggtt
     1861 gtcacggtag acggtgttga ttacccactc tggcaaacag atgccctggt agtcggcagt
     1921 ggtgcagccg ggctgcgtgc agctgtggaa cttaaacgcc gccagcaaaa tgtgctgatc
     1981 gccaccgccg ggttatatat ggggacgtcg gcatgttctg gttccgataa acagacactg
     2041 tttaccgccg ctaccgcggg caacggcgac aacttcacca aactggcaga ggcactggcg
     2101 agcggtgggg cgatggatca cgacaccgct tatgtcgaag cggtaggttc tctgcacact
     2161 cttggcgggt tgcaatatct tggtctggaa ttaccggaag atcgctatgg cgcgattctt
     2221 cgttatcaaa ccgaccatga cgaagccggg cgtgcaacct cgtgtgggcc gcgtacctca
     2281 aagttaatgg tgaaagtgct gttggaagaa gtacagcgcc tcgccattcc agtgttgacc
     2341 agtgcaacag tgattaaact gctgcatcag cgtgacgaaa acggcgaaga ccgtgtggcg
     2401 ggggcaatcc tcgcgaccgg tcatcgcgcc cataaccctt gggggctggc aattgtgact
     2461 gcgcccaatg tggtactggc aacgggcgga ccgggcgagc tttatcgcga cagtgtttat
     2521 ccacataaat gcttcggctc gctggggctg gcgctggagg aaggtctgac gctaaccaat
     2581 ctgaccgaaa gccagtttgg tataggcaca ccgcgcagca cgtttccgtg gaatttgtcc
     2641 ggcacctatg tgcaggtgat cccgtatatc tattccgtgg atgctgaggg taacgagtat
     2701 aacttcctcg cggattacta tcgcaccacc caagagctgg cttcaaacat cttccgtaaa
     2761 ggctatcagt ggccgttcca cgccacgcgg gtaatggatt ttggctccag cttgttagat
     2821 atggcggtgg cgcaagagca gcaacatggt cgtcgggtgt ttatggattt caatcgcaat
     2881 cctgaagcgg tgcctggtga tttgcccttc acattagatc gactggacga cgacgttcgc
     2941 gcgtatctgg aaaataatga cgctctggca ccatcgccca ttgaacggct gcaacgaatg
     3001 aatccgctgt ctatctcgct gtataagatg cacggttacg atctcaccac gcagccattg
     3061 cagtttgcca tgaataatca gcacatgaat agcggcattg aagtggatat ctggggacaa
     3121 acatccctgc ccggttgttt tgcggtggga gaagtagccg gaactcacgg cgttacccga
     3181 ccaggcggtg cggcgctgaa tgccggacag gttattgccg tgcgtctggc gcgctttatt
     3241 ggttgcacac aaaagcggtc tgttgagggt gatgtcgcgc agctggtagc ctcatctctg
     3301 aatatagtca gagaagttat cgctcaggca ctggtaaatg agaacggaat ggcgctggta
     3361 acggtgaaag aaaaaattca ggcgcgaatg tctgattacg ccggatttat ttgtcatgcc
     3421 gataaagttc gtcacgccac ccgtgacgcc ctgctgctaa gtgagtttgt acaacaacac
     3481 ggtttaacca tcaatcacgc aggcgaaatt accgaactat ttatgtggcg gcatatggcg
     3541 ctgacttctg ccgcc
//
LOCUS       DBKEKI010000242         3548 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.245, whole genome shotgun sequence.
ACCESSION   DBKEKI010000242 DBKEKI010000000
VERSION     DBKEKI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.245"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..692
                     /locus_tag="ACUQPX_004225"
     CDS             <1..692
                     /locus_tag="ACUQPX_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9058678.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPCELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     EMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAM
                     TALLMKDNQRS"
     gene            794..2248
                     /gene="amn"
                     /locus_tag="ACUQPX_004226"
     CDS             794..2248
                     /gene="amn"
                     /locus_tag="ACUQPX_004226"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HHV9058679.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPSKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQISIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2591..3307
                     /locus_tag="ACUQPX_004227"
     CDS             2591..3307
                     /locus_tag="ACUQPX_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHV9058680.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3352..>3548)
                     /locus_tag="ACUQPX_004228"
     CDS             complement(3352..>3548)
                     /locus_tag="ACUQPX_004228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058681.1"
                     /translation="IKRKMPYAGCSCRVFIGKLYVIATLSSVIRKRRPAILINIISLG
                     TGIFYKQYDELNIIFFIRII"
BASE COUNT          968 a          771 c          785 g         1024 t
ORIGIN      
        1 tactgggagc attgtgggtg cgcaatggca tggaggagtc cgcggaattt gaacaacagc
       61 aacataatca agcggccgcg aaaaaacgca tcccggttat cgaagctctg ttacgacatc
      121 ccggtgcttt cctgaagatt attgcactac gactgtgcga gttgctgacg atgtacattg
      181 ttactgcctt tgcacttaat tattcaaccc agaatatggg gttaccgtgc gaacttttcc
      241 ttaatattgg tttgctggta ggtggattaa gctgcctgac aattccctgt tttgcctggc
      301 ttgccgatcg ttttggtcgg cgcagggttt atatcacagg cgcgttgatc ggaacgttga
      361 gcgcatttcc tttctttatg gcgcttgaag cacaatctat tttctggata gttttcttct
      421 ccataatgct ggcaaacatt gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta
      481 ccgaaatgtt tggtgccagt tatcgctaca gtggtgctgg agtcggttat caggttgcca
      541 gtgtggttgg cggtggattt acacctttta ttgccgctgc actcatcact tactttgccg
      601 ggaactggca tagcgtcgcc atttatttgc tggctggatg cctgatttct gcaatgaccg
      661 ctttattgat gaaagacaat caacgctcgt gatagcctgg cgaagatcat ccgatcttcg
      721 ccttatactt ttgtttcaca ttcctgtgac atactatcgg atgtgcggta attgtatgga
      781 acaggagaca cacatgaata ataagggctc cggtctgacc ccagctcagg cactggataa
      841 actcgacgcg ctgtatgagc aatctgtagt tgctttacgc aacgccattg gcaaatatat
      901 tacaattggc gaattacctg atgaaaacgc ccgtaaacaa ggtctttttg tctatccatc
      961 actgaccgta acctgggacg gtagcacaac aaatccctcc aaaacgcgcg catttggtcg
     1021 ttttactcac gcaggcagct acaccaccac gattactcgc cctactctct ttcgttcgta
     1081 tctgaatgag caactaacgt tgctttatca ggattatggt gcgcatatct ctgtgcaacc
     1141 ctcgcaacat gaaatccctt atccttatgt catcgatggc tctgaattga cacttgatcg
     1201 ctcaatgagc gctgatttaa ctcgctactt cccgacaaca gaactggcgc aaatcggcga
     1261 tgaaactgca gacggcattt atcatccaac ggaattctcc ccgctatcgc attttgatgc
     1321 gcgccgcgtc gatttttccc tcgcacggtt gcgccattat accggtacgc cagtagaaca
     1381 ttttcaaccg ttcgtcttgt ttaccaacta cacacgttat gtggatgaat tcgttcgttg
     1441 gggatgcagc cagatcctcg atcctgatag tccctacatt gccctttctt gtgctggcgg
     1501 gaactggatc actgccgaaa ccgaagcgcc agaagaagcc atttccgacc ttgcatggaa
     1561 aaaacaccag atgccagcat ggcatttaat taccgctgat ggtcagggta ttactctggt
     1621 gaatattggc gtgggaccgt ccaatgctaa aaccatctgc gatcatctgg cagtgctacg
     1681 cccggatgtc tggttgatga ttggtcactg tggcggatta cgtgaaagcc aggccattgg
     1741 agattatgtc cttgcacacg cttatttacg cgatgaccac gttcttgatg cggttctgcc
     1801 gcccgatata cctattccga gcattgctga agtgcaacgt gcgctttatg acgctaccaa
     1861 gttagtcagc ggcaggcctg gtgaggaagt caaacagcgg ctgcgtacag gtactgtggt
     1921 aaccacagat gacaggaact gggagttacg ttactcagct tctgcacttc gttttaactt
     1981 aagccgggcc gtagcaattg atatggaaag tgcaaccatt gccgcgcaag gatatcgttt
     2041 ccgcgtgcca tacgggacac tactgtgtgt ttcagataaa ccgttacatg gtgagattaa
     2101 gcttcccggc caggctaacc gtttttatga aggcgctatt tccgaacacc tgcaaattag
     2161 cattcgggcg atcgatttgc tgcgcgcaga aggcgaccga ctgcattcac gtaaattacg
     2221 aacctttaat gagccgccgt tccgataata agttatgaac cttcttatca gaaatagatg
     2281 tgaaggatta agtaagacat ctggcaaata gcctgcaata aacgcgggct gcttgcttcc
     2341 ttgggcagat gcgagtttta ttatcgtctt aatgatttcc acatattaaa agcaagtatg
     2401 ctttcaaaac acaattataa aaaatcccgc caacaacatt agtttttata aaattaaata
     2461 taagattatg gctttagaat atttttattt ctaatagacg agatttttcc tgttatgata
     2521 taatatgtcg aattaacaca tgttaacgat ttaccagtaa tgtaaataaa ttttcgagga
     2581 gatcattcca gtgggacgta aatgggccaa tattgttgct aaaaaaacgg ctaaagacgg
     2641 tgcaacgtct aaaatttatg caaagttcgg tgtagaaatc tatgctgctg ccaaacaagg
     2701 tgaacccgat ccagaattaa acacatcttt aaaattcgtt attgaacgtg caaagcaggc
     2761 acaagttcca aagcacgtta ttgataaagc aattgataaa gccaaaggcg gcggagatga
     2821 aacgttcgta cagggacgtt atgaaggctt tggtcctaat ggctcaatga ttattgctga
     2881 gacattgact tcaaatgtta accgtacgat tgctaacgtt cgcacaattt tcaataaaaa
     2941 aggcggcaat atcggagcgg caggttctgt cagctatatg tttgacaata cgggtgtgat
     3001 tgtatttaaa ggaacagacc ctgaccatat ttttgaaatt ttgcttgaag ctgaagttga
     3061 tgttcgtgat gtaactgaag aagaaggtaa tattgttatt tatactgaac ctactgacct
     3121 tcataaagga atcgcggcgc taaaagcagc tggaatcact gagttctcaa caacagaatt
     3181 agaaatgatt gctcaatctg aagttgagct ttccccagaa gatttagaaa tctttgaagg
     3241 gcttgttgat gcccttgaag atgacgacga tgtacaaaaa gtttatcata acgtcgcaaa
     3301 tctctaatta tattttaaag aaatctgtct ttatggcaga tttctttaat ctcatataat
     3361 tcttataaaa aatataatgt ttaactcgtc atattgctta taaaaaatcc ccgttcccag
     3421 agaaataata tttattaaaa tggcaggtct tctttttctg attaccgaag acaaagtggc
     3481 aattacgtat agtttcccga taaagacgcg acagctacat cccgcataag gcatttttct
     3541 ctttatct
//
LOCUS       DBKEKI010000243         3530 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.246, whole genome shotgun sequence.
ACCESSION   DBKEKI010000243 DBKEKI010000000
VERSION     DBKEKI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.246"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..451
                     /locus_tag="ACUQPX_004229"
     CDS             <1..451
                     /locus_tag="ACUQPX_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0043711 - pilus organization [Evidence
                     IEA];
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis chaperone"
                     /protein_id="HHV9058682.1"
                     /translation="FVLNPANENLLRIMYIGAPLAKDRETLFFTSVRAVPSTTKREEG
                     NTLKIATQSVIKLFWRPKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKIG
                     GKVVKNQMISPFDKYQFSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            476..3075
                     /locus_tag="ACUQPX_004230"
                     /pseudo
     CDS             476..3075
                     /locus_tag="ACUQPX_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3066..>3530
                     /locus_tag="ACUQPX_004231"
     CDS             3066..>3530
                     /locus_tag="ACUQPX_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9058683.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGG"
BASE COUNT          973 a          776 c          894 g          887 t
ORIGIN      
        1 atttgtgctg aatccggcta atgagaatct gttacgcatt atgtacattg gtgcgccgtt
       61 ggcgaaagac agagaaaccc ttttcttcac tagcgtacgg gcagtccctt caacaacgaa
      121 gcgggaagag ggaaataccc tgaagattgc cacacaaagc gtcatcaaac ttttctggcg
      181 accaaaaggt ttagcgtatc ccttaggcga ggctccggcg aaactgcgtt gcacttcgtc
      241 agctgacatg gttacggtca gtaacccaac accttatttc attaccctga cagacctgaa
      301 aataggtgga aaagtagtta aaaatcaaat gatttccccc tttgataaat accaattctc
      361 tctgccaaag ggggccaaaa atagcagcgt aacgtatcga accatcaatg actacggggc
      421 ggaaacgccg caactcaact gtaaatcgta agccgtcttc agttaagaga gcgagatgta
      481 tagaactcat cgacaacaca gcctgttaag ctctggtgga gtgccatcgt ttattggtgg
      541 gctggtggtg tttgtgtcgg cagcgttcaa tgcacaagct gaaacctggt tcgatcctgc
      601 ttttttcaaa gatgatccct caatggtggc tgatttgtct cgtttcgaaa aaggacaaaa
      661 aataacgcca ggggtttatc gtgtcgatat tgttctgaat cagacaattg tagatacgcg
      721 caacgtcaat tttgttgaga taacgccaga gaaggggatt gccgcctgtt tgacgactga
      781 aagcctggat gcaatgggtg ttaatactga tgcgtttccg gcttttaaac aactggacaa
      841 acaagcgtgt gtgccattgg cggagattat tccggatgcc agcgtaacgt ttaatgtgaa
      901 taaactccgt ctggaaattt cagtaccgca aatcgccatc aaaagtaatg cccgtggtta
      961 tgtcccccct gaacgttggg atgaagggat caacgcgcta ttactgggat attcgtttag
     1021 cggggctaac agtattcata gcagcgcaga cagtgattct ggcgacagct attttctgaa
     1081 tttaaacagt ggcgttaatt taggcccatg gagattgcgc aacaattcaa catggagtcg
     1141 cagtagtggc caaaccgcag aatggaagaa tctcagcagc tatttgcagc gggcggttat
     1201 tccactaaaa ggcgaactga ccgtaggtga tgattatact gcaggcgatt ttttcaatag
     1261 tgtcagcttt cgtggtgtgc agctggcgtc agatgacaac atgctgccag acagcctgaa
     1321 agggtttgcg cctgtggtgc gtggtatcgc caaaagcaat gcccagataa cgattaagca
     1381 aaatggttac accatttacc aaacttatgt atcgcctggt gcttttgaaa ttagtgatct
     1441 ctattccacg tcgtcgagcg gtgatttgtt agttgaaatc aaagaagcgg acggcagcgt
     1501 caatagctac agcgtaccgt tttccagcgt gccattactc cagcgtcagg ggcgaatcaa
     1561 atacgcggtg acactggcga aatacagaac caatagtaat gaacagcagg agagcaaatt
     1621 tgcccaggcc acgttgcagt ggggcggacc gtggggaacg acatggtatg gtggtggaca
     1681 atatgctgaa tattaccgtg ccgccatgtt tggtctggga tttaaccttg gcgatttcgg
     1741 agcaatttcg ttcgatgcga cccaggcgaa gagtacgctg gcagaccaaa gcgaacataa
     1801 aggtcagtca tatcgttttc tgtatgccaa aacgctcaac caattgggca ctaattttca
     1861 attgatgggc tatcgctatt cgacgtcggg tttctacacc ctttccgaca ccatgtataa
     1921 acatatggat ggctacgaat ttaatgacgg tgatgatgaa gatacgccga tgtggtcgcg
     1981 ttattacaat ttgttttaca ccaaacgtgg caaactgcag gtcaatatct cccagcaatt
     2041 aggcgagtac ggttcgtttt atttaagtgg tagccagcaa acttactggc ataccgatca
     2101 acaggatcgg ctattacagt ttggctacaa cacgcaaatt aaagatctct cgctgggggt
     2161 ttcctggaac tacagtaagt cccgtggtca acctgatgct gatcaggtgt ttgcactaaa
     2221 tttttccctg ccgctcaatc tgttgctccc cagaagtaat gatagctata ccaggaaaaa
     2281 aattacgcct ggatgacctc taacaccagt atcgataacg aagggcacat tacacaaaac
     2341 ctgggtttaa cggagacact actcgatgac ggtaacctga gctacagcgt gcaacaggga
     2401 tataacagcg aggggaaaac ggctaatggt agcgccagca tggactacaa aggggcgttt
     2461 gcagatgccc gagtgggcta caactacagc gataacggca gtcaacaaca actgaactac
     2521 gctctttcag gcagtttagt tgcccattca cagggcatta ccctggggca atcgctgggg
     2581 gaaactaacg tgctgattgc agcaccaggc gcagagaata ctcgtgtggc gaacagcacc
     2641 gggctgaaaa ctgactggcg cggatatacc gttgtcccct atgccacttc ttatcgggaa
     2701 aatcgaatcg cacttgatgc ggcatcgtta aaacgtaacg tggatcttga aaatgcagta
     2761 gtcaacgtgg ttcccaccaa aggggcgttg gttctggcgg agttcaatgc ccatgcggga
     2821 gcaagggtat taatgaaaac atcaaagcag ggtataccgc tgcgttttgg cgcgatagcg
     2881 acgctggacg gcatacagac taatagcggc attattgatg atgatggctc gctctatatg
     2941 tccggtttgc ccgctcaggg agcgataact gtacgctggg gcgaagctcc cgatcaaatt
     3001 tgtcatatca gttaccagct taccgaacaa caaattaact ctgcgattac gcgaatggat
     3061 gccatatgca gataatcttt ggagaaaaat gcgtgttatt actacgacta ttttttgccg
     3121 ccgtcttaat gctatggtgc gctcaaactg ctgcttatat cgggcagtgt cataccactc
     3181 aggggaatcc gtatattggc gtcaattttg gcgttaaaac tctggaggaa gaagaaaata
     3241 cggctggagt agttaaagac aaattttatc agtggaacga atcgaatgat tattatgttt
     3301 cctgtgattg cgataaagac aatgtcagaa gtggccgatg ggcattcgcc gcggattcac
     3361 cgttagtcta tttaggcgac aactggtaca aaattaatga ctatcttgcc gccaaagttt
     3421 tattgcaggt taaaggcagt tctcctactg cggttccttt cgaaaacgtg ggcacagggg
     3481 cagatacacg atggcatatt tgcgatcccg gcggtcaacg tttaggtggc
//
LOCUS       DBKEKI010000244         3408 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.247, whole genome shotgun sequence.
ACCESSION   DBKEKI010000244 DBKEKI010000000
VERSION     DBKEKI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.247"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            122..673
                     /locus_tag="ACUQPX_004232"
     CDS             122..673
                     /locus_tag="ACUQPX_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HHV9058684.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            733..3237
                     /locus_tag="ACUQPX_004233"
     CDS             733..3237
                     /locus_tag="ACUQPX_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HHV9058685.1"
                     /translation="MYKKLKLTAISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DLENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQATNLDFPRIYLFRPIPAMNAKLTIGQYDTESSIFDSFHFSGISLKSDENMLPT
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGRVTQWQVASNSIPYLTRKGQIRYTTTMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDLGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNEISHTASWYNSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3253..>3408
                     /locus_tag="ACUQPX_004234"
     CDS             3253..>3408
                     /locus_tag="ACUQPX_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058686.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSK"
BASE COUNT         1052 a          716 c          687 g          953 t
ORIGIN      
        1 tttagataaa ctaattttat aaaaatcaca aaacaaaata taatatttta atgattggta
       61 gtccttaaat aacagttact aatcattctc ttttttactt atctttatat aaaggaatat
      121 tatgtctgct tttaaaaagt cacttcttgt tgctggtgta gcaatgattc tttccaataa
      181 cgtgtttgct gatgaaggtc acggtattgt taaatttaaa ggtgaagtta tctctgctcc
      241 atgttctatt aaaccaggtg acgaagattt aactgtaaat ctgggtgaag tagcagatac
      301 cgtactgaaa agcgatcaga aatctttggc tgaacctttc actattcacc tgcaagactg
      361 catgttgagc cagggtggaa ccacttattc aaaagcgaag gttactttca ccacagcgaa
      421 tactatgacg ggtcagactg acctgctgaa aaacaccaaa gaaactgaaa tcggtggtgc
      481 cacaggcgtt ggcgtacgta ttctggatag ccagagcggc gaagtgactt taggtactcc
      541 ggtagtaatt acattcaaca atactaacag ctatcaggaa ctgaacttca aagctcgtat
      601 ggaatccccg ggcaaagatg ctaccccggg caacgtttac gctcaggctg actataagat
      661 tgcttacgag taatcacatt aggccggggt cgcccggcca catcattaac ggattaatga
      721 taagtggaac agatgtataa aaaattaaaa ttaaccgcaa taagcgaatt gattaaaaat
      781 atttattgtt cattatccgt tctcatcatt ggttgtgcgt cagcttatgc cgttgaattc
      841 aacaaagatt taatcgaagc cgaagatctt gaaaacgtta acctttccca atttgaaact
      901 gatggccaat tacccgtagg taaatactca ttaaacgctc ttattaataa taaaaggacg
      961 ccaatccatc tggacctgca atgggtatta attgataacc aaactgcagt ttgtctgact
     1021 ccagagcaat taacattatt aggctttact gatgagatta ttgaagaggc tcagcaaaac
     1081 ctgatcgatg gttgttaccc tatcgaaaaa gaaaaacaaa ttacaactta tctcgataaa
     1141 gggaaaatgc aattatccat atctgcacct caggcatggt taaaatacaa agatgcaaac
     1201 tggacgcctc ctgaactttg ggatcatggt attgctgggg catttcttga ctacaattta
     1261 tatgcctctc attatgcacc acatcagggc gataattcac aaaatataag ttcctatggg
     1321 caggctgggg ttaatcttgg ggcctggcgc ctgcgtactg attaccagta cgatcagtca
     1381 tttaacaatg gcaaaagcca ggcgaccaac ctggattttc cgcgtattta tttgtttcgc
     1441 ccaatcccag caatgaatgc aaaactaact ataggtcaat acgatactga atcctctatt
     1501 ttcgactctt tccatttttc tggcatttcg ttgaaaagcg atgagaatat gttaccgaca
     1561 gacctacgtg gttacgcacc gcaaatcacg ggtgtcgcac aaacgaatgc aaaggtcact
     1621 gtctcacaga acaaccgtat tatttatcaa gaaaatgttc ctccaggccc atttgctatt
     1681 accaatttat tcaatacatt acaggggcaa cttgacgtca aggttgaaga agaggacgga
     1741 cgcgttacgc aatggcaagt tgcatctaat agtattcctt atctgacgcg taaagggcag
     1801 attcgctaca ccactactat gggtaaaccg accagcgttg gtggtgattc cttacaacaa
     1861 cccttcttct ggactggtga attctcatgg ggttggctga acaatgtatc cctgtatggt
     1921 ggttcagttt taacaaaccg tgattatcaa tctctggctg ccggcgttgg ttttaacctt
     1981 aactcattgg gttcattatc ttttgatgtc acacgatctg atgctcagtt gcataatcag
     2041 gataaagaaa cgggttatag ctaccgcgct aactattcaa aacgttttga atctaccggt
     2101 agccagctca ctttcgctgg ttaccgtttc tctgataaaa actttgtgac aatgaatgaa
     2161 tatatcaatg acactaacca ttacacgaat tatcagaatg aaaaagagag ttatattgtc
     2221 acgtttaacc agtatcttga atcattaagg ttaaatacat acgtaagttt ggctcgtaat
     2281 acttactggg acgccagcag taatgtgaat tattcattat cacttagccg cgattttgat
     2341 ctcgggccat taaaaaacgt ctccacttct ctaacattta gccgaataaa ctgggaagaa
     2401 gacaatcagg atcaactgta cctaaatatt tcgattccct ggggaaccag tagaacattg
     2461 agctatggta tgcaacgaaa tcaggataac gagatttcgc atactgcttc gtggtataac
     2521 tcttccgatc gaaataattc ctggagcgtt tctgcttcag gcgacaatga tgaattcaaa
     2581 gatatgaaag cgtcactacg cgccagttat cagcataata ccgagaacgg tcgtctctat
     2641 ctctccggga catcacagcg agacagttat tattctctga atgccagttg gaatggttca
     2701 ttcacggcga ctcgtcacgg agccgctttc catgactata gtggtagtgc tgactcgcgt
     2761 tttatgatcg acgcagatgg tgcagaagat attccgttga acaataaacg cgcggtaact
     2821 aatcggtatg gcatcggagt tattccatca gtcagcagtt acataacaac atcattaagc
     2881 gttgataccc gaaatcttcc agaaaatgtg gatatcgaaa actcggttat caccaccacc
     2941 ttaaccgaag gtgctattgg ctacgccaaa cttgataccc gcaagggcta tcaaatcatg
     3001 ggggttattc gcctagcaga tggtagtcac ccaccactgg ggattagcgt aaaagataaa
     3061 accagccata aagaattagg actggttgct gatggcggct ttgtatacct caacggcatt
     3121 caggatgaca gcaaacttac tttacgctgg ggtgataaat cctgttttat tcaaccaccc
     3181 aatagcagca acttaaccac cggaacggtt attttaccgt gtattagcca aaattaattc
     3241 aggatgaaac taatgcgcta cttaaacaca aagaatctca ttgcagcagg agtattactc
     3301 gcctgcatga gcagcattgc ctggggtgcc atcatcccgg accgtacccg tatcataatg
     3361 aatgagtcag ataaagggga agcattaaaa cttactaatc aaagcaaa
//
LOCUS       DBKEKI010000245         3347 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.248, whole genome shotgun sequence.
ACCESSION   DBKEKI010000245 DBKEKI010000000
VERSION     DBKEKI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.248"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..67
                     /locus_tag="ACUQPX_004235"
     CDS             <1..67
                     /locus_tag="ACUQPX_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HHV9058687.1"
                     /translation="AQQQQLTSWQEQGHDIFPLGI"
     gene            127..234
                     /locus_tag="ACUQPX_004236"
     CDS             127..234
                     /locus_tag="ACUQPX_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HHV9058688.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(215..946)
                     /gene="yehW"
                     /locus_tag="ACUQPX_004237"
     CDS             complement(215..946)
                     /gene="yehW"
                     /locus_tag="ACUQPX_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HHV9058689.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQATLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGFSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(951..1877)
                     /gene="yehX"
                     /locus_tag="ACUQPX_004238"
     CDS             complement(951..1877)
                     /gene="yehX"
                     /locus_tag="ACUQPX_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HHV9058690.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(1870..3027)
                     /gene="yehY"
                     /locus_tag="ACUQPX_004239"
     CDS             complement(1870..3027)
                     /gene="yehY"
                     /locus_tag="ACUQPX_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HHV9058691.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3034..>3347)
                     /locus_tag="ACUQPX_004240"
     CDS             complement(3034..>3347)
                     /locus_tag="ACUQPX_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HHV9058692.1"
                     /translation="IKAAAQQTSGVNAAMAYGTDGPVAALGLQTLSDPQGVQPIYAPA
                     PVVRESVLKEYPQMAQWLQPVFASLDAKTLQQLNASIAVEGLDAKKVAADYLKQKGWA
                     K"
BASE COUNT          832 a         1034 c          838 g          643 t
ORIGIN      
        1 cgcccaacag cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg
       61 tatttaatga ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag
      121 tgcattatgc gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc
      181 ggaattggtg gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct
      241 gcaccagcct ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc
      301 cctggatcac atacgcggta ttaaatccgc taaacccgat gatgatgggc gtacccagcg
      361 tactggcccc taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc
      421 gcacgcccgc cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac
      481 ctcgactcat tcccatacct ttcgcaactt ctgtcacgct gggatcaatc gctcccagcc
      541 cggcaagtgt cgcctgcagg atgggcagca caccgtaaag gatcaaggcg ataatcgctg
      601 gttgcagacc aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag
      661 tctgtccaac ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag
      721 taacagcaat tccggcacct gtgccaatga tcaccgcaaa caaactcgaa attcccacca
      781 gccagaaatg agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca
      841 gttgtgggaa caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagcgcca
      901 caaaaagagc aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc
      961 accagcagat cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc
     1021 agcacctcgc atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct
     1081 gccagtgcct caccttctgc ccgttcttcg cgacgcacgt aatccgccac gctacgtaac
     1141 gaaagcaggc gcacccccag ttcactacgt ccaaaaaact ggcggacaaa atcattcgcc
     1201 ggacgagtca gcatcgtcag cggattcccc tgctgcacca cttcaccgtg atccatcaat
     1261 accagatgtt ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatagtg
     1321 cgccccagca aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg
     1381 tccagtgcgc caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcacgc
     1441 gccactccca cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgcaaa
     1501 tttgactcca gccccagtag cgccattaat tcgtcgatac gatcgtcaat ccgcgcccgc
     1561 gaccattttt gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgtgggaac
     1621 aggccaatag attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag
     1681 cgaatttctt ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc
     1741 attttcaggg tggtggattt gccggagcct gatgtgccaa tcagcaccga aaaactccct
     1801 tcctgaaaat tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca
     1861 tggctaaatt caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc
     1921 gtcggtaagc accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc
     1981 gctgcttagc agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc
     2041 aatcaccgcc atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg
     2101 taacgccagc ggtaactgaa catgcaggaa acgctgcgcc ccgctcatcc ccatcgctct
     2161 ggcgctctcc agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag
     2221 cggcagcaag gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat
     2281 gccgagcttc cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag
     2341 gccaaagagc gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc
     2401 ctgccgcgca gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg
     2461 cagcaccgca ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg
     2521 acggttggcg tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca
     2581 cagcggaata atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat
     2641 tcggcggatg gcatcgctac aggccagcaa tgccagcgcc gcagccagcc agaaaccact
     2701 gccgaggctg gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc
     2761 agctccccac accaacaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc
     2821 ttttttagcg gggacaaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca
     2881 gatcgtttgc ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg
     2941 cgcgtaactg ataaacggca gcgccgctgc gattgccgtc agcaacaaca gcagcgccag
     3001 aacaggatta atacgtagat aagtcacggg aaattacttc gcccaccctt tttgtttcag
     3061 gtagtcggca gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg
     3121 ctgcaatgtt tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg
     3181 atactccttc aacaccgatt cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc
     3241 ttgcggatcg cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc
     3301 cattgcggca ttaacgccag aggtttgctg ggcagcggct ttaatcg
//
LOCUS       DBKEKI010000246         3278 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.249, whole genome shotgun sequence.
ACCESSION   DBKEKI010000246 DBKEKI010000000
VERSION     DBKEKI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.249"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1417..2133
                     /gene="nanC"
                     /locus_tag="ACUQPX_004241"
     CDS             1417..2133
                     /gene="nanC"
                     /locus_tag="ACUQPX_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HHV9058693.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2153..3259
                     /gene="nanM"
                     /locus_tag="ACUQPX_004242"
     CDS             2153..3259
                     /gene="nanM"
                     /locus_tag="ACUQPX_004242"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HHV9058694.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSYAPMGMAGHVTFVHNGKAYVTGGVNQNIFNAYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
BASE COUNT         1034 a          634 c          680 g          930 t
ORIGIN      
        1 ctgggttagc aataatccaa catttttatc ctgattatgt ttataaaagc gaacgtttgc
       61 ttaataactt acgtaagtgg accagttctt ctgagcgaac ttaaatggag tagcgatagt
      121 tacttataat aactttgcca tacaaacgcc atgctcttgc atgctatgta cctttatata
      181 tttatcaatc tgatacgaat ctaataataa ccctcatcat taatgcaact aatcttatct
      241 atatatcatg tgatatgttc tgtaacaagt aaaatcaaca taaaaaattc agcaaaattt
      301 aatatttgtt caactagccg tctcaaatat gacattatta gttaactgca tttgccacaa
      361 aatggctatt tcatggaacg cagtaatttc ttagtggcta ttatcatgct aattcttaga
      421 tccctagcga ttattcctgc ctggttcgtc agcttaggcc atgagattgt tacattcctt
      481 tttatcctgg agatatgggg gaacgcaggc gacgcttttt catctacatc cgttggtaaa
      541 tatagctttg gtcactacga cttaagcctt cctcaatcac agctattcgc cgggcgatac
      601 ggcaaacaaa ctaagtgaaa catcatcctg aaaacaccaa catcaacaag cctctccaga
      661 tcgacatcag gagtgaccag ttacaagcca caaaccaaga aacatatttc atgatgagaa
      721 ttatgctcag cctgatggcg ggtaaaccct tattttcacc actctcactg agttagcttg
      781 caaagctttc tttgtttaga ttctaaataa attatttata aaacatatag ttaaaagaaa
      841 atctttagtt tttgaagacc accgcaagtg ttcgtctggc ttcacatggc atcttcttct
      901 tagaaaaaga tcgacatatt ttgtgacgcg aattgcaaat ctggttttgt tgtatggatt
      961 gcgtgatttt tgatctggta taacaggtat aaagatgcac cacgatagtc aatgagactg
     1021 ggcatctcgt aatctatggc aaacatcact tcagttcttt cacatcgggt gatgaaaatg
     1081 cacttcagtc tgaaaggaat atgaaaatga gatcaacaga cactctattt tatgactctg
     1141 ggtaaaatgg gttgagtaag tgatataact tacgaatatt caaatcaatt aaaaatcaga
     1201 agagatttta tactcaggta tttaatctgg atctctgttt atttaaataa tgtgaaaaga
     1261 gaattttcac aggagacctt atacaaaaaa tataaaatac agttacccgt tgccaaagac
     1321 actataagcc tggcaaaaaa atattacaca acataaatgc taattgttta tgcgggcttt
     1381 gtattgcttt ctgtatccta caaatgagtg aaatttatga aaaaggctaa aatactttct
     1441 ggcgtattat tactgtgctt ttcgtcccca ttaatttctc aggctgcgac actggacgta
     1501 cgtggtggat atcgtagtgg aagccacgcc tatgagactc gactcaaagt cagtgaggga
     1561 tggcaaaatg gatggtgggc aagcatggaa agtaatacct ggaataccat tcatgataat
     1621 aaaaaggaaa atgccgcact caatgatgtt caggttgaag ttaattacgc aattaaactt
     1681 gatgatcaat ggacggtgcg cccgggaatg ttaacgcatt ttagcagcaa cggcacacgc
     1741 tacggaccct acgtaaaact gtcctgggac gcgacaaaag atcttaattt tggcattcgc
     1801 tatcgttacg actggaaagc ttaccgacaa caagacttat ccggtgatat gtctcgtgat
     1861 aacgttcatc gttgggatgg atatgtcact taccatatta atagtgattt caccttcgca
     1921 tggcaaacga cgctatacag caaacagaat gattatcgct atgcaaacca caagaaatgg
     1981 gcaacggaaa atgcctttgt tctacaatac catatgacgc ccgatattac gccatacata
     2041 gaatatgact accttgaccg ccagggtgtt tacaacggca gagataattt atcggaaaac
     2101 agttatcgca ttggtgtgtc atttaaactg tagtagacag gagacagtca caatgaataa
     2161 aactataatg gcgcttgcta tcatgatggc ttcatttgcc gcaaacgcgt ctgtattacc
     2221 ggaaactcct gtgccattta aaagtggtac cggagcaatt gataacgaca ctgtctacat
     2281 tggtttaggt agcgcaggta cagcatggta caagctagat acacaggcca aagataaaaa
     2341 atggactgcg ttagctgcat tccctggtgg accgagagag caagcaacct ctgcatttat
     2401 tgatggcaat ctgtatgtgt ttggcggcat tggcaaaaac agcgagggat tgactcaggt
     2461 atttaatgac gtacacaaat acaaccccaa aaccaatagc tgggttaaat tgatgtcgta
     2521 cgcgccgatg ggcatggcgg gtcatgtgac ttttgtacac aacggcaagg cttatgttac
     2581 tggcggtgtt aaccagaata tcttcaatgc ctattttgaa gatctcaacg aggctggaaa
     2641 agattcaacc gctatagata aaatcaacgc ccactatttt gacaaaaaag cagaagatta
     2701 tttcttcaat aagtttctgt tgtcttttga tccctcaaca cagcaatgga gttacgctgg
     2761 cgaatcgccc tggtacggaa cggctggtgc ggcggttgtg aataaaggtg ataaaacctg
     2821 gcttattaat ggcgaagcca aaccaggatt gcgaacggat gccgtatttg aacttgattt
     2881 caccggtaat aatttaaaat ggaataagct tgatcccgtc tcatcaccag atggcgtcgc
     2941 tggcggtttt gcggggataa gcaatgattc tcttatattt gccggagggg ccggattcaa
     3001 aggttcacga gaaaattacc agaacggtaa gaactatgcg catgaaggcc tgaaaaaatc
     3061 atatagcact gatattcatc tttggcataa cgggaaatgg gataaatcgg gtgaattatc
     3121 gcaaggtcgg gcctacggag tatcattgcc ctggaataat agtttattaa ttattggcgg
     3181 tgaaactgca ggcggcaaag cggtgacgga ttcagtttta atttctgtga aggataataa
     3241 agtcacagta caaaactaac gcttcagggc cccggtaa
//
LOCUS       DBKEKI010000247         3219 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.250, whole genome shotgun sequence.
ACCESSION   DBKEKI010000247 DBKEKI010000000
VERSION     DBKEKI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.250"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..411
                     /locus_tag="ACUQPX_004243"
                     /pseudo
     CDS             1..411
                     /locus_tag="ACUQPX_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_213850417.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            996..1712
                     /locus_tag="ACUQPX_004244"
     CDS             996..1712
                     /locus_tag="ACUQPX_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058695.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGSYEINDVYVRK"
     gene            1946..2074
                     /locus_tag="ACUQPX_004245"
                     /pseudo
     CDS             1946..2074
                     /locus_tag="ACUQPX_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2264..2668
                     /locus_tag="ACUQPX_004246"
     CDS             2264..2668
                     /locus_tag="ACUQPX_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058696.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2731..3219
                     /locus_tag="ACUQPX_004247"
                     /pseudo
     CDS             2731..3219
                     /locus_tag="ACUQPX_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
BASE COUNT          909 a          653 c          738 g          919 t
ORIGIN      
        1 gaagacccag atatgaacag gcacattcag agttacttta aagagcttac tgaacgtctg
       61 aggtgttaga gccatgactg ccgcagcatt gctggcggaa atgcctgaat cgagcagcct
      121 ctcgagacgt gaaatcagcg cacttgtcgg tgttgctcag gtaaaccggg actcgggaac
      181 tctgcgcggt cgacgaacca ttttcggtga ttgtgctggg gaagaacagc tctgtacatg
      241 gcggcgcttg aggcaccccg gtttaatctg gtgataaagg cgttttacat gcgcctactt
      301 gcagccggaa acgccaaaaa agtggcactg gttgcctgta tgcgtaagtt gctgaccatt
      361 atgaacgcaa tgctcagaaa aaacgaagaa tggaatgaat cgtatttatg aaaactggat
      421 tgtgggccat aaatattcca aaaaaatagc ccgctccgtt tattaccgga acgggcgaga
      481 gcatgacatt gtctgtaatt ataatgagac tgctgattct acttattttt aatttcaaga
      541 aaagcgtttt ctacaaagaa ttatgagatt taatgttttg tttagacaat attatcattc
      601 tgttcaataa tgaaagtctt catcaacaac aagtaaaaac aacgtccctc tcatcgaacc
      661 ggacttgcag atttcccgca tccggctctc ttgcaacaca tcagaccttc acgttcgggc
      721 ggttatgggg aaggtccagc aagctgtatg agccctaact gtttgaatac cagtagatta
      781 ccagaaactt ctatcgattg aagacattac caactagcgt tactaaatgt tgaccaagtt
      841 ttgttcactt gtctggtttt gatagtagca ttctgcagag tattttgata tgataaatat
      901 gcattaacaa tgatattatt gttaatttcc tagcatgaaa attaaaagat caacttttta
      961 tctcgaatat tttttacatt atcagttggt ttcttatgaa tgacaattca ttgcttagaa
     1021 attcttcatt attcatcgcc tatatgggat gtgtagggtg ggtcagtgct tattcttatg
     1081 gctggggaac ctctttttac tatggatttc catggtgggt tgttggtgca ggtttggatg
     1141 atgttgccag aagtttactc tatgctatca tagtaatggg tatacttttt accggttggg
     1201 ggattggtat tttatttttt cttcttataa agaaaagaag taaaatacaa gacttgagtt
     1261 tttttagact attttttgct attaccctac tgtttttccc tgtaatattc gaacttttaa
     1321 ttctcaaaca atatttcata cttccattat cactttcatg cattattagt tcattggtta
     1381 tttcaataat aattcgtata tatggacgga tcttttctgt atcatgtttt tcagatatcc
     1441 cttttgttag agaacaccgc attaagttaa ttatggctgg ttttttggtt tatttttggc
     1501 ttttttcctt tcttgttggc tggtataaac ctcaattaaa aaaagaatat cagatgcttt
     1561 gttataataa tagttggtat tatgttcttg ctcgttatga ttcacggctc gttttgtcta
     1621 gttcatttaa agatgacagt aacagattct taatatttaa tacagaacag tcgggatctt
     1681 atgaaatcaa tgatgtttat gtcagaaagt aataatcctc tcccacaaag tgaattaaat
     1741 gaatatataa tgcgttcgaa gtatatgtat tgagctgtca tactgagaaa caaaccttag
     1801 ggcagcacaa ttaaagccag aaacaactat gcaagaaagt tactaaataa gtgctcggaa
     1861 cagtaaaaca ctcagggggg actcagtcca gattatgtaa tcagataaat tgccaaacat
     1921 cacaaatcac cagccggact gagtgatgcc gagcattgca ccaatacccc gtgatgaacg
     1981 atgcctgatg cagaaagctc cctataaaac aaagaacaaa aactacgacc ggcgattcac
     2041 cgtcatactg atgctgcatc gtggtgacac tgtctgtcac tttcatgttg actgaacaac
     2101 tctgctgcga agaaggtgta ccgccggcgg gatttgattg tccggcgtct agcttccact
     2161 tccctgatgg aactgaaagt tgttatggcc ggtcagcttg ccctgtccgc aggacatgag
     2221 gtgctgtgac ggacagcacc agtaacggaa agtgacaatg aaaatgaata ctgcgctgat
     2281 agttgcacta atgtgtatgt ggtatgcggt acccgctgct gcaaaagaga ctttactggc
     2341 tatgccacgt aattcaacag aacattgcta tgcggagatt aatgtgcacg gaccatatgg
     2401 ggtgtatttc cgagtggtcc cgcatccccc cgggggaaag agctgggtcg aatgtaacag
     2461 tgactactat tactctgata aaccaccggg agtacagata ctcggaaccc gtgcagggtg
     2521 tcgggtatac ggaatctgcg ggaccacctc gacactgcat gtggctggaa gaggggttgt
     2581 gtgtattaaa aatatatgca gtccacgtgg gatgataatt cacaggatac ggaaacgtcc
     2641 tgtagtggct gtaagcgatg agatgtgatc ttacccagca atagtggaca cgcggctaag
     2701 tgagtaaact ctcagtcaga ggtgactcac atgacaaaaa cagtatcaac cagcaaaaaa
     2761 acacgtaaac agcattcacc tgaattttgc agtgaagccc tgaagcttgc tgaacgcatc
     2821 ggtgttgctg ccgcagcccg tgaactcagc ctgtatgaat ctcagctcta tgcgtggcgc
     2881 agcaaactgc agcaacagat gacctcgtct gagcgtgaaa gtgaactggc cgctgaaaat
     2941 gcccgcctga agcggcaact ggcggagcag gcagaggaac tggctatcct ccaaaaggcc
     3001 gcgacatact ttgcgaagcg cctgaaatga agtatgtctt tattgaaaaa catcaggctg
     3061 agttcagcat caaggcaatg tgccgcgtgc tccgggtggc ccgcagcggc tggtatacgt
     3121 ggtgtcagcg gcggacaggg ataagccctc gtcagcagtt ccgtcagcac tgcgacagcg
     3181 ttgtcctcgc ggcggccttt acccggtcaa aacagcgtt
//
LOCUS       DBKEKI010000248         3163 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.251, whole genome shotgun sequence.
ACCESSION   DBKEKI010000248 DBKEKI010000000
VERSION     DBKEKI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.251"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..2065)
                     /locus_tag="ACUQPX_004248"
     CDS             complement(<1..2065)
                     /locus_tag="ACUQPX_004248"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HHV9058697.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPLGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLIPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGKEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKPMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDI"
     gene            complement(2086..2712)
                     /locus_tag="ACUQPX_004249"
     CDS             complement(2086..2712)
                     /locus_tag="ACUQPX_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HHV9058698.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT          844 a          790 c          658 g          871 t
ORIGIN      
        1 caatatcttc cagccccaga cgctgcaatg tttcgcgggt tggacaacca agctgtgggt
       61 cccagcccat ttctttgtag aacatcgtga gcgaagcatg catatcgtca cgatccattt
      121 tgtcagtacc ttcagtaaag accgggatct gcggatcctt gtcgaaaacc caggaacaga
      181 taagatcgtg ttcattacgc atatctttgg tttgcatcgg ttttaccgtg taggcacgat
      241 gcaacgtaaa gatacgctct gcagctaaat ctaatttttc ctgagtcatc tcttcgccag
      301 tgatcgcttt gaagaatttg gcttcaagcg ccagatcgcc ccggtaatta cggcttttca
      361 gcggcgaaac ggtcattggc cagacccagt tgcacagggt gacggcgtta tgcaaacaga
      421 cccgcaacag cgaccacttt gcatatttaa tttttgcgtc attgattggc gtgtagtttt
      481 tggtttcatc gtaagcatct tcagaaccaa aaagttcttt cgccacttca cgttgcagtt
      541 tcaatggcaa gccggaacca ataaagttga tatgggtatg cgtcatgcag tcacggttga
      601 acatacagtt aacaatggaa ccgacctgcg ctgacgcttc attggcatgg tgaaccggat
      661 agccaaacgg cgaccagagt ttatttttcg cgtagcccca gtactcttta cccaaattcc
      721 agcgttctgc gatggcatat gaaccatcag ccaggtgact cagctcaccc acacgatgcg
      781 ccagacggta gtaaaaatct ttaatgaagt taacgtcacc cgcttccagt tgatcccagc
      841 gaatttctgc atactcttca gctggcagaa cacgcttgaa cacacctttg ctgtaacaat
      901 aagtaaagtc gcgatgcaac tgcccgtagt tacaccatag gccgtagtca tcgaacaaat
      961 tcagaccaac caggttacca atcacacgac catcgtcttt atcttcaaaa tctttcggcc
     1021 cgttcgggaa gatggtggta tggacaaagt ttgctacaca agtgttacca cctgtgctgg
     1081 gcacgccaaa ctctttcacc cgaggaatat tcatttgggt catacaacgg atcgggcaag
     1141 agtggcagcc gctcattttt actgtgtatt tctctgccgc cggtcctaag tcaaaaacgg
     1201 atttataggt acgaaagccg accgtattct gattgcctgg cggaatttca cccgtttcaa
     1261 tcggaccacc ttcagccgcg ccccaaaaca gccctttacg tgctgtccag cgtgacttgg
     1321 gatctgaata ctctgcccac gattgtggcg tacttggcac gacatggttg ttattcgcac
     1381 caataagttc agtcatcatg taatcattga gacgcttcat ctcctgacga tcggcaatgt
     1441 tgaccccttt cgtcccttca accgcaatcg ctttcaggtt tttcgaaccc attattgcgc
     1501 cagttcccgc accgccgctg tggttacggc tattcaacat gccagagaga ggaataaggt
     1561 tttccccagc ctgaccaata gccgccacac aggtttccgg actggtcaat cgacaaattt
     1621 cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc ggctttttcc aggctaactt
     1681 tgtcatcttt aatcttcagc cataccggtg atttcgcctt cccttcgata ataatgacgt
     1741 cgtatccagc gaatttcatt tgcgctgcaa aaaaaccccc catatgggca tcgacgacta
     1801 aatttccttt ggtaaaagta gaaagtgagg tgatatttac gcgagaacta cagggggcac
     1861 cagatccagt taatgggccg gtagcaaaga ctaatttatt cgcttcatcg aaaggtttcg
     1921 tgcctagcgg tacttcgtca tacataattt tgtagccgaa gcccatgcca ccgacaaaac
     1981 ttttaaactt actggaatct tcgagggtaa tatttcctgt cgtgagattg actcttaata
     2041 tattacctgt ccaaccgtta gccatgattt ttttctttgc aagattcaca cagtaatatc
     2101 tttccactcg ataattttta acgcccctgt tgggcaggcg tttgcgcatt caccgcataa
     2161 cacacatttc gaggattttt tactttcggt atttacggtg gccatcatcc acgggcatgc
     2221 cgtagtacag gcgctacagc caatacaacg tttatgatcg acggtaatac agccttcttt
     2281 ctgctgccag gtaatcgcac caatcgggca gacgttcatg cattgcggct ctttgcattg
     2341 acggcaggtg tccgcggtat agttgagatc gccatacagg ccgccgccag agccaacccc
     2401 gttgtcgcca aagaaataat tgcgatggat tttgatacgg gagaagaatg tccctactga
     2461 gccatcgttg aagttggtac aagagatttc acagcggtgg caacccgtgc atcgcgctcg
     2521 ctgggtcacc agcacccctt tgggtgtatt tatcaaccct actgtgccgc tatcaatatc
     2581 ttcctgtttg caacccaaaa gtgacagtaa cgcaggggca attgttagac ctgcaaggcc
     2641 ttttcctgat atacgcaaga attctaatcg cgttaagcca atatctaata gtggacgatc
     2701 aaccgggttc atttatttcg catcctcttc gcaaacacag tgctgacaat tcagcagcct
     2761 gatattaagc attcaactaa tacgctgctg actctgtccc caacaccaga tattttccct
     2821 aacccgatag tgtatgagaa aagatttaac gccgataaac taatccttta tggttaggag
     2881 aaaaaataat gtgattattc tgagccctta acattgatcg ttatcaatta aaattacaaa
     2941 gcaagaaatt ataagcgaac tgatatttat tatcatttga aataaattta acttaagcga
     3001 caatcatttg tgacgttcat tgcagaaaat aaaaataaca ttatcgctat aaattaaaat
     3061 acataccgaa attcgtataa tttcagcagt tgttatttag aatgattaac tggaccgcaa
     3121 ctgaagcaag aatatcagga tgaagcttac cgcctcatcc tga
//
LOCUS       DBKEKI010000249         3109 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.252, whole genome shotgun sequence.
ACCESSION   DBKEKI010000249 DBKEKI010000000
VERSION     DBKEKI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.252"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            112..360
                     /gene="ymgC"
                     /locus_tag="ACUQPX_004250"
     CDS             112..360
                     /gene="ymgC"
                     /locus_tag="ACUQPX_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HHV9058699.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            693..2216
                     /locus_tag="ACUQPX_004251"
     CDS             693..2216
                     /locus_tag="ACUQPX_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HHV9058700.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPLLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            2348..2567
                     /gene="ymgF"
                     /locus_tag="ACUQPX_004252"
                     /pseudo
     CDS             2348..2567
                     /gene="ymgF"
                     /locus_tag="ACUQPX_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
BASE COUNT          860 a          608 c          657 g          984 t
ORIGIN      
        1 ttctaattat taagtataag tttatagaaa actcattcat cttatttttg tctgtcgctt
       61 tagactatac acagcataat tttattgggt taatatttct acgaggctga catgaataat
      121 tcaatcccag agagatttat ttttcaatgt gctttgttta aaaatctcga gagggaggtg
      181 ttcatgacgc atgggtatgt tgatagtcat attattgatc aggctttacg cttgcgttta
      241 aaagatgaaa ccagtgtgat tctttctgat ctctatctgc aaatattgca gtacattgaa
      301 atgcataaga ctacactaac ggatatcatt attaatgaca gggaatccgt gctctcttag
      361 aacttgagta aggttttaga ataaacatca gctgtatcac catgctgatg caaagtgagc
      421 atttcaggcg ttatgcttcc ttattatgtc cgcaatatca ggtgtcaaga atggagagtt
      481 ctcgctctcc attcttgaca cctgatatcc cgcctaactt attttgtact gcctacatta
      541 tgctcaataa ttaatgggtt agatcaagat ggagaaaatt ttatcttcgg cgtggatatt
      601 tcttatgctc tctaccatgc atctctgtag atgccagcgc gagcgaatga atggcatttt
      661 catgtattaa accctttggc cgggacaagc aaatgcgcaa tacactcata cccatcctcg
      721 ttgcgatatg cttgtttatt accggtgtcg ctattttgaa catccagctc tggtattccg
      781 caaaagcaga gtacctggcg ggagcaagat atgccgccaa caatatcaat catatacttg
      841 aagaagcgtc acaggcgact caaacagcgg ttaacattgc cgggaaggaa tgcgacctcg
      901 aggagcaata tcagcttggc actgaagcag ctctgaaacc tcacctgcgc acaatcatca
      961 ttctcaaaca gggaatagtc tggtgtacat ccctgcctgg gaatcgggtc ctgctgtctc
     1021 gtattcctgt tgtcccggac agtaatttac tgttggctcc ggcaatcgac accgttaata
     1081 gattacctat cctgctctat cagagccaat ttgcagatac gcgcattttg gttacgataa
     1141 gtgatcaaca tattcgcggg gcgcttaatg tacccttgaa aggggtaagg tatgtattac
     1201 gcgtggcgga tgacattatt ggaccaacgg gtgatgtgat gacgcttaat ggacattatc
     1261 cctataccga gaaggttcac tccacaaaat atcatttcac tattatcttt aacccgccgc
     1321 tactctttag cttctacaga cttatcgata aaggctttgg gattttaata tttattctgt
     1381 taatcgcctg cgccgctgcc ttcctgcttg atagatattt caataaaagc gcaacgcctg
     1441 aagagatcct gcgacgggca ataaataatg gggagatcgt ccctttttac caacctgtgg
     1501 taaatggtcg ggaagggaca ttgcggggag ttgaggtgtt agcccgctgg aaacaacctc
     1561 acggtggata tatttcaccc gcggcattta ttccacttgc tgaaaaatcc ggattaatcg
     1621 ttccgcttac acaaagcctg atgaatcagg ttgccagaca gatgaacgct atcgcgagta
     1681 aactgccgga aggttttcat attggaatta attttagcgc ttcgcatatt atttcgccga
     1741 cgtttgtcga cgagtgttta aatttccgtg acagttttac ccgccgcgat ttaaaccttg
     1801 ttctggaagt caccgagcgt gagccattga atgttgatga aagtctggtt cagcggttga
     1861 acattctgca tgaaaatggt tttgtcatcg cgctggatga tttcggtact ggctactcag
     1921 ggctttctta tcttcatgac ctgcatattg attatatcaa aattgatcac agtttcgttg
     1981 gccgcgtaaa cgcagaccca gaatcaaccc gaattctgga ttgtgtattg gatctggcgc
     2041 gtaaactttc gatcagtatc gtcgctgaag gtgtcgaaac gaaagaacaa cttggctatc
     2101 tgaaccaaaa taatatcaca tttcagcagg gttattattt ctataaacct gttacataca
     2161 tcgacctggt caaaattatc ctttctaaac cgaaggtgaa ggttgtggtt gagtgaaaat
     2221 tgatcagtaa ggccatagtg cggtgtaatt atagacagct aattagctcg ttgcctcttg
     2281 ttactattgt tcattatttt gtttgctata attgtttgaa agttttgaca ggattgccat
     2341 tagtagcatg aacaatagta ataatctgga ttatttcact ctctatatca tcttttccat
     2401 tgcatttatg ctgatcaccc tcctggtcat ccttattgca aaacccagta ccgggctggg
     2461 agaagtgctt gtgacgataa atttgcttaa tgcccttgtt tggctggcga tcaatctggt
     2521 taatcgattt aagagaaaga ctcgtcagcc acagggatca gcaataatct ttcagtttct
     2581 cactgtcagt atgcggctga atgggttgct ggcagtgaac gcctggatca ttgaaggaaa
     2641 ggaattattg cgcaaatagt tgtcaacctt ggtgttatca cagttgtttt tatatatcac
     2701 cgaaataatt accatggcaa ctattaataa ttttgatgtc gaagagttat ttgttaaaca
     2761 aaatagtcac cccaaagtga tcaatgtcat gaaaataagg tgaaaaatga taatgccgac
     2821 ttatttatca tttatatatt gtcgctgttt atctatttaa attttttgtg cttttgtttt
     2881 tgtctttacc ctccgttttg gtaatctgag ctcataggtt atttgaccag gaatgtttgt
     2941 taatccaatg aatggatggt taatctatga aactgaaaaa actccccggg tttagtttgg
     3001 gacttattgc tctggcggtg ggtaatgcat atgctgaaca gtttattcag cacgatgaat
     3061 ataaacatgc tgtggcaata aaagatggaa aagtaaaaga tattgataa
//
LOCUS       DBKEKI010000250         2964 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.253, whole genome shotgun sequence.
ACCESSION   DBKEKI010000250 DBKEKI010000000
VERSION     DBKEKI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.253"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            32..562
                     /locus_tag="ACUQPX_004253"
     CDS             32..562
                     /locus_tag="ACUQPX_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HHV9058701.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETN
                     WTKDNGKLNAVRLVQE"
     gene            569..802
                     /locus_tag="ACUQPX_004254"
     CDS             569..802
                     /locus_tag="ACUQPX_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HHV9058702.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFGELQKMFSLTQRDN
                     AMLNGRVMKLSQQVQHLSERTERLSQLYSENRR"
     gene            complement(1925..2473)
                     /locus_tag="ACUQPX_004255"
     CDS             complement(1925..2473)
                     /locus_tag="ACUQPX_004255"
                     /inference="COORDINATES: protein motif:HMM:NF013884.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HHV9058703.1"
                     /translation="MSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIPSFYGGVL
                     GQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLLILLSYLN
                     KEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIGCVIMLYV
                     ISPIVFHVMNTFTFKRNIFFYL"
     gene            complement(2612..2704)
                     /locus_tag="ACUQPX_004256"
                     /pseudo
     CDS             complement(2612..2704)
                     /locus_tag="ACUQPX_004256"
                     /inference="COORDINATES: protein motif:HMM:NF015365.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          927 a          520 c          680 g          837 t
ORIGIN      
        1 gggaagaata tcagactgaa aggagcgttt tatgagcaat cttttacaga cgggtgcgga
       61 gttcgagaaa aagctgaaag agagagccga atctacagag aaaatgctga acgacgagtt
      121 caggaagctc gaagaatctg taaacaggga actgacctca aacgaatctt taatcaggaa
      181 cgctatcaac gaccatacat cagccctgaa agaacttctg gagaggtatc agaaaacgac
      241 cgtcgatacg atggacgcac actggaaaac cgtactgaaa atgagcgtga aacgctggct
      301 gtggctgatt atcgtctcag tactgatgtt tgccacgacc gggagcctgc tgtggtacca
      361 gggcatgaag ataaacgcga acatgaatat tctccgcgag cagaaagaga gtctggagaa
      421 gctgaacgcg aagacatggg gcgtgcgtta tcacgaggac agcaacggac gttttctggt
      481 gttgccgaag gggatgaaag ccgaaacgaa ctggacgaag gacaacggca aactgaacgc
      541 agtgagactg gtacaggagt gacagagcat gacggagctg gaaagacatt tgctgaacgc
      601 attagagcaa ctgcaacagg actatatgca gcggctgaac gaatgggaga gcgccttcgg
      661 ggaattgcag aaaatgtttt cgcttacgca acgggacaac gcgatgctga acgggcgggt
      721 catgaagttg agtcagcagg tgcagcactt gagcgagcgg acagaacgct tgagccagtt
      781 atacagcgag aacaggagat aagggatgaa cggctgatgc aggaacgcga acatgagtta
      841 tctctggaac gggagcgcca gccggaaatt caggaacgga cactggatgg cccttcgctg
      901 ggatggtaac cagagacagg ctaaaatgtt ggccgcgcgt ttccggtgca ttttttggga
      961 taatggttag ttgggggcac cgggaacgcg cttcagtcag accttgctga aaacacagga
     1021 acgcgttaaa acgcatacgg gcgcgattct ggtgtgtctt tgttgctgtg ttttgccgtt
     1081 attgccagat cgcgctcatt cccctgaatt tctgtttttc cgctccggtt ccgtcttctg
     1141 cctttccgaa agtatgaaaa aatccgtttc agcagtctct ggctggcaat tcctgcgtga
     1201 ttcacatggc tgcatagcta tgcatgtggt taaaatttat cgggtgcgat cgcggcagtt
     1261 tttcgggtgg tttgttgcca tttttacctg tctgctgccg tgatcgcacc gaacgcgttt
     1321 tggcggtgcg tacaatgaga tgttatggta aattcaagtc ttagatttta tgatgtttaa
     1381 ttagcttttt ttgcgtttat aatgttcatc ttttttatta aaattgattc aatctttgat
     1441 gtgggtatta atattttctt tgacaaatag aaagttaaaa aaaacaccat taatagtata
     1501 aataaatacc caaaaacaga agcgttatta atttcaatta ttgatgttaa tctgtatatt
     1561 actacatgct gaatctacat ggatatgtag atgcatttag catatgagtt gaaaaaatag
     1621 aggttaaatt tcttattaat tattaatgct gctttgtaaa tgataatgct tatgcacatt
     1681 aaaggtatta ttgataactg gtgattggct atgtttgttt tgaactcatt aggccctatt
     1741 aataatactg tcaatataca tataacaacc gatagaataa ctgtggtgtt tttttgtaat
     1801 ttatttttgt atttaaatgc aagcatccca accataaact caggaagtcg gcaagcaaaa
     1861 aaccaaaatg ggaagtttaa aaatgatgat gagatatttg atattgggaa tgttgtaatt
     1921 gttatcacaa ataaaaaaat atgttgcgtt taaatgtaaa ggtattcata acgtgaaaaa
     1981 ctatcggtga tatcacgtac agcataataa cgcacccaat aaaccactcg cctgtaagga
     2041 aaagaaaatc ataagagaaa tgggcgttta atataccatc aagggcaaaa aatactggaa
     2101 ttattctagt gtactggcct cctgttgtca ttattgatac gagagggact gtattaatta
     2161 tctctttatt taaataagac aataaaatta agagtaaaga gatgaaaaag taagtgataa
     2221 tataaggggt aaggatcctt actcctttgt caaggaaata atcaatggta ttctttctat
     2281 ctagtgaaat aaatgccaaa tatccagata taaaaaaaaa caagctcaca cctacctggc
     2341 caagaacccc tccataaaat gatggtatga aagatagcct ggtttggcta aatatgtagc
     2401 cataatgaga tataataaca aaaatgcagg ctgttactcg caatgtatca attgacggat
     2461 ctctattaga cataaccttt atttcgctgt tcaaattatc gcctttagtt gatgatgtaa
     2521 aattaacgct ataaataagc tacacattct agagcagcga aaggatgatt caagtatttt
     2581 taataataaa acgcattgat aatgtttatg ttcagatggt gctgatagct ttgtcatgct
     2641 gttccactga tgttgataac gacagagact tttgtccttt tcttgttaga tgctgcctca
     2701 gattcggatt atggcgttaa aggggtttga cgctcaaccg aacggtttca cacgttaaga
     2761 gctttttgtc tgataaatca aaatataatg gacattgaaa aacgccctca aaacggtgtc
     2821 tttttaatcg aagattggca caaaacggac accaccgtcc attagtcgat tttttagacg
     2881 gcttttctcg cctaacgtga gttttcgttc ggttgagcat cagacccctt ttatattgta
     2941 gatttctatt tttagacagt tgga
//
LOCUS       DBKEKI010000251         2955 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.254, whole genome shotgun sequence.
ACCESSION   DBKEKI010000251 DBKEKI010000000
VERSION     DBKEKI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.254"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(371..1168)
                     /locus_tag="ACUQPX_004257"
     CDS             complement(371..1168)
                     /locus_tag="ACUQPX_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9058704.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGNKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGISFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1178..1729)
                     /gene="ybcL"
                     /locus_tag="ACUQPX_004258"
     CDS             complement(1178..1729)
                     /gene="ybcL"
                     /locus_tag="ACUQPX_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HHV9058705.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKISVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1898..2230)
                     /gene="emrE"
                     /locus_tag="ACUQPX_004259"
     CDS             complement(1898..2230)
                     /gene="emrE"
                     /locus_tag="ACUQPX_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HHV9058706.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2575..2889)
                     /gene="fliE"
                     /locus_tag="ACUQPX_004260"
     CDS             complement(2575..2889)
                     /gene="fliE"
                     /locus_tag="ACUQPX_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HHV9058707.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
BASE COUNT          876 a          660 c          565 g          854 t
ORIGIN      
        1 tttctgggac gtttcaacgc cccattttta ataatactca gtgaacaatt aacgtatcaa
       61 ttagatttaa taacaacgat atccgtacct aacctgatat ctaatgtcat aagcatccct
      121 ttaattatcc cctcaacctc ttgtaaattt cccactcttg agctccacat agtccaaacg
      181 ccggatgcaa aaaaaatcac acgtgcatct ctgaaattgt gatggaggaa gtgagtatca
      241 attatatgtg cttataaacg ttcccgattg gcacagaagt caccggattg ttcaggctcc
      301 aataggttga gtatgtcgta tttattttcc aaaataaaaa tcgatgctgt tgctccttaa
      361 taaaatactt ttactctctg gacgcttttc ttagctctct tggtgttatc ttaaaacgat
      421 tatggaacct ttcagtaaaa cgagaaggac acttgtaacc attttccctg gcaatctcgc
      481 tgatgggttt tactgtcgtt tgtatagcag acaacgcatt atttaacctc acgtcgtcca
      541 atatactttg aaaacttatt ccctcgcttg ccagacggcg atgcaatgtg gaaacagaaa
      601 tgtagagata tctcgctacc ttgtttgctg tccattttgt tccaggttcg gatagcatta
      661 ggttataaca acgacttatt aatgattgtt tactatctga taaaagtaaa caattaacat
      721 gatgtgctcc taatgaaagt agaacgccca ttgctaagtg ttcctgaatt tgcgttgaga
      781 agcctcggga aatagatgtt tttagttgct cccaacaata tattaactcg gggttctgag
      841 gtaagcagaa acctgtcgtg ttacgaattt gatcagttat cgcataaaat ttttgaaact
      901 tttcaattag atcaatggga taataaagca tttctgcaag ataaagacct gcttctggat
      961 aattcacaat ataaaattca tatccacagg gaaataatat tatctgatta ttatcaacag
     1021 ttagactatg cgtctcccaa ttgataactt tatttccctg acggatacga cataaagctg
     1081 gcataagagg cttaaccctg tgaatctcat gatgtttatg catccgtatt tcttcaatct
     1141 ttaggttaga cttatctttt gccagcatac tcttacccta ctttatctca taaactggtg
     1201 ttatctcagc ggttgcaatt ttattggcat taagcatata accaactaac gctccgctgg
     1261 aattagaatc cacagaaatc ttatcagttt ttagagccca tactttaaac tggtaatgat
     1321 ggggtttatc tcctttggga ggacatgctc caccaaatcc tgcataacca aaatcatttc
     1381 ggccttgaac agcaccagtc ggaagttttg taccatcacg tcttcctgca tcagtgggca
     1441 aatatgttac agttgctgga atattagcaa cagtccagtg ccaccatcca ctgcctgtag
     1501 gagcatctgg atcatacaca gttacggcaa agcttttggt gccttcagga gcaccagacc
     1561 aggttaatga gggcgatgta ttaccacctt cacacccaaa tccagaaaat acatgagacg
     1621 ttgtaagttg ctctcctgtt tttatttcat tactagtgac ctgaaatgct gcggcctgcg
     1681 cagaaaatgt tatgaatgcc aatacacttg aaacgataag ttttttcata aaaacctcat
     1741 tgttgtgacc tatcgttatt ttatttgatg tgccttgatc tcattatgca taaaggcgca
     1801 atgatcgcgc aaatgcaatc ataattataa aggtgccccg aacattagcc ataatatgca
     1861 caaatcagat tcctgttatc tcaaggataa ttatttttta atgcggtgca cttcgtgaca
     1921 acaagttaat gactaacaca ccggcacaaa tcaacatcat gcctatgata gctggcaggt
     1981 ccagtcgttg accaaaaaat ccccaggaaa gcaaactaat cagaacgata ccgactcctg
     2041 accagatagc ataagcaatc cctgtaggta tataagccag cgtctgagct aataaccaga
     2101 atgatgcaca atagcaaata attgtgccaa cagagggcca taaccgtgta aacccctctg
     2161 aaaacttcat taaggttgta ccaatgacct ctgcaagtat tgcgccacca agataaatat
     2221 aagggttcat agcatattct ttcctgttca tactggagag aattgtacta cagtttgaac
     2281 acaactcacc gttttcatcc tggtgtatgt gtatccaagg ggctctggcg tggcaagaga
     2341 taaaggcagg aacaataatg atcagaagga accccaggct aacgaatgag ttcactcttc
     2401 ttgggagcaa agcgaagaca aaacatgtta tcgcgtttac ggatcgtgga tggcagaaaa
     2461 caaccaggat caggtatcca ttttaaacca gaaattgagt gagtttgccc catccatgcc
     2521 ccacgctgtg ggatcggata taattaaaca agcatagtca acaggttaca caacctacac
     2581 ctgcatggac atcacttcct gatacgccgc caccagctta ttacgcacct gaatccccat
     2641 ttgcatagaa actgaggctt tttgcatatc ggtcatcaca tcgtttagcg ccacgccggg
     2701 ttcaccgagg gtgaattttt ctgcctgcgt gcgggcgact gtttgtgtat cgcttatgcg
     2761 atcgagcgcg gcgtgcagct gcccggcaaa actaatggtc ggttgcggca gtgattcctg
     2821 cgcacgcgca ctcatcgccg tagcctgtaa ctggctgata accccttcaa tcccctgtat
     2881 cgctgacatt ttcgtctccc ggataatttc tggtaacaaa gcctaccagt aagtcaataa
     2941 gacaaaggcg ctaaa
//
LOCUS       DBKEKI010000252         2867 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.255, whole genome shotgun sequence.
ACCESSION   DBKEKI010000252 DBKEKI010000000
VERSION     DBKEKI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.255"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..200)
                     /locus_tag="ACUQPX_004261"
     CDS             complement(<1..200)
                     /locus_tag="ACUQPX_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HHV9058708.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYP"
     gene            complement(219..2096)
                     /gene="bglF"
                     /locus_tag="ACUQPX_004262"
     CDS             complement(219..2096)
                     /gene="bglF"
                     /locus_tag="ACUQPX_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HHV9058709.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2230..>2867)
                     /gene="bglG"
                     /locus_tag="ACUQPX_004263"
     CDS             complement(2230..>2867)
                     /gene="bglG"
                     /locus_tag="ACUQPX_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HHV9058710.1"
                     /translation="IPLEVMATCDRLISLAQERLGKLQDSIYISLTDHCQFAIKRFQQ
                     NVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQMSGN
                     MEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASINDSDE
                     SLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
BASE COUNT          774 a          757 c          640 g          696 t
ORIGIN      
        1 tccgggtaac ggtgataaaa atcgatggcg acatctttga tattctcttt ccccaggatg
       61 cgcggttcca tttttcccat tacgccatga ggctgtaaat ctgaggtcgt gatccctttg
      121 ccatcttcca gccaggcacc ttccacctga ttggcagctg ttgcgccacc ccaaagaaat
      181 gtttctggaa atgctttcat aattaactcc ttttatcgtt agcgaatgat ggataacagc
      241 ggttcacctg cgcttatctg cgccgtgccg tggggtaata cgtctgtaaa atcatcgcta
      301 ttactgatta ataccggcgt cgtcagatca aatccggcct cgcgaatagc agggatatca
      361 aaagaaatca gccgatcgcc tgtattgacc ttgtcaccca cgttgacgtg agcggaaaag
      421 aatttgccgt ccagttttac ggtgtcgata ccgacatgaa tcaggatctc cacaccatca
      481 tctgactcaa tgccaatggc gtgtaatgtg gcgaacaacg aagcaattcg acccgcaacc
      541 ggagaacgca cttcaccaac cgagggcaga atggcgatac ctttacccaa caggccactg
      601 gcaaacgtgg tatcagcgac gtgaatgagc ggcacaatct ctcccgtcat cggtgaacag
      661 ataccgccct gctcaggtgg tgtaataacc tctggtgttt tctcttgcgg ggcaccctgc
      721 gctggctgac gtttactggt gatgaaatga agcatcaacg taccaacaaa tgcgcaacct
      781 atggcaatga caccgccaat aacgctggcc cagacggtga aatcaatccc cgttgacggg
      841 atggtctgca tgaaggtgaa aatacttggc aaaccaaagg agtaaacttt cgtttgcgcg
      901 tagccaatga tggtggcccc caatgctcca ctgatacagg cgataacaaa ggggtactta
      961 cgcggcaggt tgacgccata taccgctggt tcggtgatac caaacagacc cgtcaacgcc
     1021 gctgatcccg ccaccacttt tttctgcgca tcgcgttcgc agaggaagac gccgagcgcc
     1081 gccccgacct gcgccataat ggcgggcatt aacagcggga tcatggtgtc gtagcccagc
     1141 acggtgaagt tattgataca cagcggcacc aggccccagt gcagtccgaa catgacgaag
     1201 atttgccaga agccgcccat taccgcgccc gcaaatgcag gaaccgcctg ataaagccag
     1261 agataaccgg cggcaatcat ttcgcttatc caggttgata gcggccccac cagcagaaag
     1321 gtgacgggtg tgataaccat cagacatagc aatggtgtga agaaattttt gattgccgac
     1381 ggtaaccacg cattaagtcg gcgttccaga atgctgcaca accaggcaga aaaaataatg
     1441 ggaataaccg atgacgagta attcaacaat gtgaccggaa tacccaggaa atccagcccc
     1501 ggcgcatccg ctttttgccc gttctcgaag gcagtcagaa ttaatggatg cactaacgct
     1561 ccaccaatca ccatggcagt aaatggatta ccgccgaagc gtttgcccgc ggtgtatccc
     1621 aggattatcg ggaagaacca aaacaaggca tcactggcgc tgaataaaat taaataagta
     1681 ccactttgtt cggtcgtcca ctgaaaagtg agcgccagag ccagcatacc tttcaggatc
     1741 ccggttgccg ccatcagacc gatcagaggc gtaaaaatac ctgaaataac ataaacaaag
     1801 cggtttagca gattaccttt ctcatcattt tccggtgcct gttgcgcttt ttcgccaagg
     1861 cctgccacgc tgttaaccgc caggaagaca tcggccacat ggttacctat gaccacctga
     1921 aactgaccac cgctttccac caccataata ataccggggg tctttttcag tacctcagct
     1981 tgcgctttgc tttcatcctt taacttaaaa cgtaatcgcg ttgcgcaatg catcagactc
     2041 acaatgttat ctgcgccccc gactcctgcg actatttttc tggctaactc cgtcataact
     2101 tgccctctac cgctttgcgg caaaactcca aaaaaaaacc tgaaaaaaac ggcctgacgt
     2161 gaatcaagca atttttttca ggttttgccc gcttagtgcg gtaacaatcc tttactcagt
     2221 aataatattt cagtgttctt tgcgcacgcg ctctatattt atggctaaaa acataatctc
     2281 tgcgggtgaa attttacgtt gatactgcaa accaataaaa atggcgatcc gttccgcaca
     2341 ttgccatgct tgcgggtaat tttgttttac tgcttgttgt aatgattcat cactatcgtt
     2401 aattgaagca tgttcaagaa tacgccagga taaaaacttc agatgtgtaa ccagtcgctg
     2461 ataactcaag ctttcttcct ggtaattaag gctgaactga aattttatta attgcagcat
     2521 ttcgcgcatt aactgcgtga cacctgcaac atcctccata tttccgctca tttgggcact
     2581 gaccagatgc atggcaataa agcccacttc atctttcggt aactgcacgc ccaaccgttt
     2641 atcaataatg gttaacgctt cttcccctag ctggaactct ttcgggtaaa gccgctggat
     2701 atcccacagc aacgggttgg gcagcaacac gttttgctga aagcgtttaa tcgcaaactg
     2761 gcaatggtca gttagcgaga tataaatact gtcctgtaat tttcccaagc gctcctgcgc
     2821 taaagagata agacgatcac aggttgccat cacctcaaga ggaatat
//
LOCUS       DBKEKI010000253         2842 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.256, whole genome shotgun sequence.
ACCESSION   DBKEKI010000253 DBKEKI010000000
VERSION     DBKEKI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.256"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..114)
                     /gene="mokC"
                     /locus_tag="ACUQPX_004264"
     CDS             complement(<1..114)
                     /gene="mokC"
                     /locus_tag="ACUQPX_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HHV9058711.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQ"
     gene            700..1866
                     /gene="nhaA"
                     /locus_tag="ACUQPX_004265"
     CDS             700..1866
                     /gene="nhaA"
                     /locus_tag="ACUQPX_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HHV9058712.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1932..2831
                     /gene="nhaR"
                     /locus_tag="ACUQPX_004266"
     CDS             1932..2831
                     /gene="nhaR"
                     /locus_tag="ACUQPX_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HHV9058713.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
BASE COUNT          652 a          648 c          713 g          829 t
ORIGIN      
        1 ctggccagtt cggatgtgaa cctcacagag gtcttttctc gttaccagcg ccgccactac
       61 ggcggtgata cagatgacga tcagggcgac aatcatcgcc ttatgctgct tcattgctct
      121 cttccccttg accttacggt cagtaagagg cactctacat gtgttcagca tataggaggc
      181 ctcgggttga tggtaaaata tcactcgggg cttttctcta tctgccgttc agctaatgcc
      241 tgagacagac agcctcaagc acccgccgct attatatcgc tctctttaac ccattttgtt
      301 ttatcgattc taatcctgaa gacgcctcgc atttttgtgg cgtaattttt taatgattta
      361 attatttaac tttaatttat ctcttcatcg caattattga cgacaagctg gattattttt
      421 gaaatattgg cctaacaagc atcgccgact gacaacaaat taattattac ttttcctaat
      481 taatccctca ggaatcctca ccttaagcta tgattatctg ggcttagggt cactcgtgag
      541 cgcttacagc cgtcaaaaac gcatctcacc gctgatggcg caaattcttc aatagctcgt
      601 aaaaaacgaa ttattcctac actataatct gattttaacg atgattcgtg cggggtaaaa
      661 tagtaaaaac gatctattca cctgaaagag aaataaaaag tgaaacatct gcatcgattc
      721 tttagcagtg atgcctcggg aggcattatt cttatcattg ccgctatcct ggcgatgatt
      781 atggccaaca gcggcgcaac cagtggatgg tatcacgact ttctggagac gccggttcag
      841 ctccgggttg gttcactcga aatcaacaaa aacatgctgt tatggataaa tgacgcgctg
      901 atggcggtat ttttcctgtt agtcggtctg gaagttaaac gtgaactgat gcaaggatcg
      961 ctagccagct tacgccaggc cgcatttcca gttatcgccg ctattggtgg gatgattgtg
     1021 ccggcattac tctatctggc ttttaactat gccgatccga ttacccgcga agggtgggcg
     1081 atcccggcgg ctactgacat tgcttttgca cttggtgtac tggcgctgtt gggaagtcgt
     1141 gttccgttag cgctgaagat ctttttgatg gctctggcta ttatcgacga tcttggggcc
     1201 atcattatca tcgcattgtt ctacactaat gacttatcga tggcctctct tggcgtcgcg
     1261 gctgtagcaa ttgcggtact cgcggtattg aatctgtgtg gtgtacgccg cacgggcgtc
     1321 tatattcttg ttggcgtggt gttgtggact gcggtgttga aatcgggggt tcacgcaact
     1381 ctggcggggg taattgtcgg cttctttatt cctttgaaag agaagcatgg gcgttctcca
     1441 gcgaagcgac tggagcatgt gttgcacccg tgggtggcgt atctgatttt gccgctgttt
     1501 gcatttgcta atgctggcgt ttcactgcaa ggcgtcacgc tggatggctt gacctccatt
     1561 ctgccattgg ggatcatcgc tggcttgctg attggcaaac cgctggggat tagtctgttc
     1621 tgctggttgg cgctgcgttt gaaactggcg catctgcctg agggaacgac ttatcagcaa
     1681 attatggtgg tggggatcct gtgcggtatc ggttttacta tgtctatctt tattgccagc
     1741 ctggcctttg gtagcgtaga tccagaactg attaactggg cgaaactcgg tatcctggtc
     1801 ggttctatct cttcggcggt aattggatac agctggttac gcgttcgttt gcgtccatca
     1861 gtttgacagg acggtttacc ggggagccat aaacggctcc cttttcattg ttatcaggga
     1921 gagaaatgag catgtctcat atcaattaca accacttgta ttacttctgg catgtctata
     1981 aagaaggttc cgtggttggc gcagcggagg cgctttattt aactccacaa accattaccg
     2041 gacagattcg agcgctggaa gagcgcctgc aaggcaaatt atttaaacgc aagggacgtg
     2101 gtctcgaacc cagcgagctg ggagaactgg tctatcgcta tgccgataaa atgttcacct
     2161 taagccagga aatgctggat attgtgaact atcgcaaaga atccaattta ttgtttgacg
     2221 ttggcgtggc tgatgcactt tccaaacgcc tggtcagtag cgtacttaac gccgcagtgg
     2281 tagaaggcga gcccattcat cttcgctgct tcgaatccac ccacgaaatg ctgctggagc
     2341 aattaagtca gcataaactg gatatgatca tttctgactg tccgatagac tctacgcagc
     2401 aggaaggcct gttctccgtg agaattggcg aatgtggcgt gagtttctgg tgtacaaatc
     2461 caccaccaga aaaaccgttc ccggcttgtc tggaagaacg gcgacttttg attcctgggc
     2521 gacgttcaat gttagggcgc aaattgctta actggtttaa ctcccaggga ttaaacgtag
     2581 aaatcctcgg cgagtttgat gatgccgctt tgatgaaagc ttttggtgcg atgcacaatg
     2641 caatcttcgt tgccccaacg ctttatgcat atgactttta tgccgataaa actgtcgtag
     2701 aaattggtcg cgtcgagaat gtgatggaag agtaccatgc tatttttgct gagcggatga
     2761 ttcagcaccc ggcggtacag cgaatctgca atacggatta ttctgcgctt tttagtccag
     2821 cggtgcgtta atcggcagct cc
//
LOCUS       DBKEKI010000254         2835 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.257, whole genome shotgun sequence.
ACCESSION   DBKEKI010000254 DBKEKI010000000
VERSION     DBKEKI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.257"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..122
                     /locus_tag="ACUQPX_004267"
     CDS             <1..122
                     /locus_tag="ACUQPX_004267"
                     /inference="COORDINATES: protein motif:HMM:NF041288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HHV9058714.1"
                     /translation="PEKAFTGELIYMHGNMIARKKRKYCGAACAEKDKMAHEL"
     gene            197..493
                     /locus_tag="ACUQPX_004268"
     CDS             197..493
                     /locus_tag="ACUQPX_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HHV9058715.1"
                     /translation="MSIIECEIELDVINDSLAEFKCGNTLHSFIQISQKENVTVIIDD
                     GYVLGTFDCCVCAIKEIALLTARIEEADKKYGISYQRLKELSHIASSVRFFYAI"
     gene            517..1992
                     /locus_tag="ACUQPX_004269"
                     /pseudo
     CDS             517..1992
                     /locus_tag="ACUQPX_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
     gene            2009..2422
                     /locus_tag="ACUQPX_004270"
     CDS             2009..2422
                     /locus_tag="ACUQPX_004270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058716.1"
                     /translation="MKSKFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLK
                     EKRAKEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAG
                     RNALWIELEGVEYAFPDPQTQNETQQAGYDENIPV"
     gene            2473..2661
                     /locus_tag="ACUQPX_004271"
     CDS             2473..2661
                     /locus_tag="ACUQPX_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HHV9058717.1"
                     /translation="MYKITATIEKEGGSPTSWTRYSKTKLTKSECEKMLSGEKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            2766..>2835
                     /locus_tag="ACUQPX_004272"
     CDS             2766..>2835
                     /locus_tag="ACUQPX_004272"
                     /inference="COORDINATES: protein motif:HMM:NF019442.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HHV9058718.1"
                     /translation="MYLTLKEWNARQLRPRNPETVRR"
BASE COUNT          882 a          642 c          640 g          671 t
ORIGIN      
        1 tacctgaaaa agcttttact ggcgagctta tttatatgca tggaaacatg attgccagaa
       61 agaaacgaaa atattgcggt gcagcttgtg ccgaaaaaga caagatggca cacgaacttt
      121 aattaactga atattcgaaa ctgaatttat gccagcaatg gcagggattc actcaacctt
      181 aaaaaaggaa acagcaatga gcattattga atgtgaaatt gaacttgatg ttattaacga
      241 ttctctcgcc gaatttaaat gtgggaatac attacatagt ttcattcaga tttcccaaaa
      301 agaaaacgtc acggtaatta ttgatgacgg ttacgttctg ggaacatttg attgttgtgt
      361 atgtgccatt aaagaaattg cattactcac agctcgcatt gaagaggctg acaagaaata
      421 cggtatcagt tatcaacgac tgaaagaact ctctcatatc gcatcatcag ttcgtttctt
      481 ttatgcaatc tgaatatgag ttgaggttaa aaaacaatga gcaccgataa acaagtttac
      541 ccactgtatt acgaagcaaa aaatgacaaa gtaagaaaac gcctcggtat taaaggcggt
      601 ttttactggg ctgaagcgaa aaaattatcc attgccatct cccgtggtgc tgttgcgatt
      661 gacgatgctg gctacgatga agatgacttt aaaaaacctg ttcgcgtcaa tttgcccgtt
      721 gttgatgacc tcccaccaga aggcgtgttt gatacggaat tctgcaaccg ttacgaaaaa
      781 ggcggggaag atggcatcac aatggtattt atcgcatctt ctccctctgt tcaggacaaa
      841 ccagccagca ctgacaatac caacgtcaac ggcgaagaca tgacagaaat tgaggagaac
      901 atgctcctgc cagtttccgg tcaggaactg cccattcgtt ggcttgcgca acacggcagc
      961 gaaaaaccgg taacgcacgt ttcacgcgac gaactccagg cattacacat tgctcgggat
     1021 gaagaactac cggctgttac tgctctggct gtttcgcata aaaccagtct gctcgacccg
     1081 ctggagattc gcgacctcca caaactcgtg cgtgatactg acaaagtttt ccctaatcct
     1141 ggcaattcaa atctgggact gatgactgct tttttcgaag catatctgga tgccaactac
     1201 accgatcgcg gtctgctgac aaaagagtgg atgaaaggaa atcgtgtttc gcgtatcact
     1261 cgcacggctt ccggtgctaa tgctggcggc gggaacctca ccgatcgcgg cgaaggtttc
     1321 gttcacgatc tgacgtcact ggcgcgcgac gtagccactg gcgtactggc tcgttcaatg
     1381 gacgtggaca tttataacct tcatccggca cacgctaaac gtgtcgagga aattatcgct
     1441 gaaaataaac cgcccttttc tgttttccgc gacaaattca tcaccatgcc tggcgggatg
     1501 gattattccc gcgccatcgt ggttgcgtca gtgaaagatg caccgattgg tatcgaggtc
     1561 atccccgcgc acgtcactga atatctgaac aaggtactga ctgaaaccga tcatgccaac
     1621 cctgatccgg aaatcgtgga tattgcctgc ggtcgctcct ctgccccgat gccgcaacgt
     1681 gtaacagaag aaggaaaaca ggatgatgaa gaaaaaccgc aaccatcttg cgcaatggca
     1741 gatgaacagg caacggctga aacagtggaa ccggatgcaa ctgaacatca tcaggacacg
     1801 cagccgctgg atgctcagtc acaggtaaat gctgttgatg cgaaatatca gaaactgcgg
     1861 acagaactcc atgaagcccg gaaaaacatt ccgcccaaaa atcctgtcga tgcagacaaa
     1921 ttactggctg cctctcgcgg agaatttgtt gaagggatta gcgacccgaa tgattcgaaa
     1981 tggattcccg gtcatcatat ctcctcaaat gaagtcaaaa tttaaaaact ttcttcctca
     2041 tatcgagttt cttaacctgg gaggtgaaca cctcggcatc gatacctcac gggataacag
     2101 tgaattattt gccaacgacg gtacaccgac atggctaaaa gaaaagcgtg caaaagaaat
     2161 cgcacttgat gaaattatcg agttgctcaa caagcaccat gggggcacca gtaacgacgc
     2221 caaacgcgca aaagctgatt tgctggaaaa aacattcacc tcccgctcat gggaaagaat
     2281 caaaggtatg gactggccga caatcaaagc tggtcgtaat gcactatgga ttgaactgga
     2341 aggcgttgaa tatgcattcc cggatccaca aacacaaaac gaaacgcaac aagcaggata
     2401 cgatgagaat attccggtgt aaaaccaagt gcattttgga cacggttttt gaaaatatta
     2461 atggaggtgt tcatgtataa aattacagcc actattgaaa aggaaggtgg atctcctacc
     2521 agctggacaa gatactcaaa gacaaaacta acgaaatcag aatgcgaaaa aatgctctca
     2581 ggggaaaaag aagcaggcgt ttccagagag cagaaagtaa aactgataaa ttttaattgc
     2641 gagaaacttc agtcctcgtg aatttcatta tatccagatt agaacttcat agctgattat
     2701 taaaaatcaa ccacacccgc cagtattctg tatatttact ggcggtcata ttgcagagat
     2761 atgacatgta tttgacgctc aaagaatgga acgcccgtca gttacgtcca cggaacccgg
     2821 aaacggttcg ccgct
//
LOCUS       DBKEKI010000255         2832 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.258, whole genome shotgun sequence.
ACCESSION   DBKEKI010000255 DBKEKI010000000
VERSION     DBKEKI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.258"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            180..1406
                     /gene="eutH"
                     /locus_tag="ACUQPX_004273"
     CDS             180..1406
                     /gene="eutH"
                     /locus_tag="ACUQPX_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HHV9058719.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMR
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            1403..2815
                     /gene="eutA"
                     /locus_tag="ACUQPX_004274"
     CDS             1403..2815
                     /gene="eutA"
                     /locus_tag="ACUQPX_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HHV9058720.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGVAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPSRIR"
BASE COUNT          546 a          773 c          850 g          663 t
ORIGIN      
        1 acgggattgc acatgtaggg cggataaggc gttcacgccg catccgccag tggcgcggtg
       61 caattgccgg atgcgacgct tgccgcgtct gatccggcct tcgggattgc acatgtaggg
      121 cggataaggc gtttacgccg catccgccaa taaataacaa ctaacaggga gtaaaggcga
      181 tgggaattaa cgaaatcatc atgtacatca tgatgttctt tatgctgata gctgccgtag
      241 acaggatcct gtcgcagttc ggcggttctg ctcgtttcct cggtaagttc ggtaaaagta
      301 tcgaaggatc aggcggtcag ttcgaagaag gctttatggc aatgggcgct ctgggtctgg
      361 cgatggtcgg tatgaccgcg ctggcaccgg tactggctca cgtactcggg ccggtaatta
      421 tcccggttta cgaaatgctc ggtgcaaacc cgtcaatgtt cgccggaaca ctgctggcgt
      481 gcgatatggg cggcttcttc ctcgccaaag agctggcggg cggcgacgta gcagcgtggc
      541 tatactctgg gttaattctc gggtcgatga tggggccaac gattgtgttt tccattccgg
      601 tggcgctcgg cattatcgaa ccttctgatc gtcgttatct ggcgctcggc gtgctggcgg
      661 gcattgtgac cattccaatt ggctgtatcg ccggtggcct ggtggctatg tactccggtg
      721 tacagatcaa cggccagccg gtggaattca cctttgcgct catcctgatg aacatgatcc
      781 cggtgcttat cgtcgcggta ctggtggcgc tggggctgaa attcatcccg gaaaaaatga
      841 tcaacggctt ccagatcttc gccaaattcc tcgttgcatt gatcaccctc ggtcttgctg
      901 ccgcggtagt taagtttctg cttggctggg aactgatccc cggtctggac cctatcttta
      961 tggcccctgg cgataaaccc ggtgaagtga tgcgcgccat tgaagttatc ggttctatct
     1021 cctgcgttct gttaggggcg tatccaatgg tgctgctgct gacccgctgg tttgaaaaac
     1081 cgctgatgag cgtcggtaaa gtgctgaata tgaacaacat cgcggcagcc ggcatggtgg
     1141 caacgcttgc caacaacatc ccgatgttcg gcatgatgag gcagatggat acccgcggca
     1201 aagtcatcaa ctgcgccttc gccgtttccg ctgctttcgc cctgggcgac cacttaggct
     1261 tcgccgctgc caacatgaac gccatgatct tcccgatgat tgtcggcaag ttgatcggcg
     1321 gcgtaacggc gattggcgtg gcgatgatgc tggtgccaaa agaagacgcg accgcgacta
     1381 aaaccgaagc ggaggcacaa tcgtgaacac tcgccagcta ttgagcgtcg gtatcgatat
     1441 cggcaccacc accacccagg tgattttctc ccacctggag ctggttaacc gtgcggcggt
     1501 gtcgcaggtg ccgcgctacg aattcattaa acgcgaaatt agctggcaaa gcccggtgtt
     1561 ctttacccct gtcgataaac agggtggttt aaaagaagcg gaactgaaat cgctgatttt
     1621 agagcaatat caggctgcgg gcgttgcgcc ggaaagcgtt gattctggtg ccatcatcat
     1681 caccggtgaa agcgcgaaaa cccgcaatgc tcgcccggcg gtgatgacgc tctctcagtc
     1741 gctgggcgat tttgtcgtcg ccagcgccgg gccgcacctc gaatccgtga tcgccggtca
     1801 cggagccggg gcgcaaaccc tttctgaaca acggctgtgt cgggtactga atatcgatat
     1861 cggcggtggc accgcgaact acgccctgtt cgatgccgga aaaatcagcg gcactgcctg
     1921 tctcaacgtc ggtggtcgcc tgctggaaac cgacagccag gggcgcgtgg tttacgctca
     1981 taaaccgggg cagatgattg tggatgagtg cttcggtgca ggcactgacg cccgttcgct
     2041 gaccggcgcg cagctggtgc aggttacccg gcggatggcg gcgctgattg tcgaagtgat
     2101 tgacggaacg ctttcgccgc tcgcgcaggc attgatgcaa accggtttgc tgcccgcagg
     2161 tgttacgccc gaaatcatta cgctttctgg aggcgtgggc gaatgttatc gccaccagcc
     2221 cgccgacccg ttctgttttg ccgatattgg cccacttctg gcaacggcgc tgcatgacca
     2281 tccgcgcttg cgtgagatga atgtgcagtt tccggcgcaa accgtacgcg ccacggtgat
     2341 tggcgcgggt gcacataccc tttcgctctc tggcagcaca atctggctgg agggcgtaca
     2401 actgccgctg cgcaatttgc cggtggcgat cccgattgat gaaacggatc tggtgagtgc
     2461 ctggcaacag gcgctgcttc agctggatct tgatcccaaa actgacgcgt acgtgctggc
     2521 gcttcccgcc tcgctgcctg tgcgttacgc cgcggtactg acggtcatca acgcgctggt
     2581 cgatttcgtc gcgcgttttc cgaatccgca tcccctgctg gtggtggccg ggcaggactt
     2641 tggtaaagct ctgggcatgt tgttgcgccc acagctacaa caactcccgt tggcagtcat
     2701 tgacgaagtg attgtccgcg cgggggacta tatcgacatt ggtacgcctc tttttggcgg
     2761 atcggttgtg ccggtgacgg tgaaatcact cgcatttcct tcccgaatac ggtagttatc
     2821 aggtatggtg ct
//
LOCUS       DBKEKI010000256         2767 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.259, whole genome shotgun sequence.
ACCESSION   DBKEKI010000256 DBKEKI010000000
VERSION     DBKEKI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.259"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..484
                     /locus_tag="ACUQPX_004275"
     CDS             <1..484
                     /locus_tag="ACUQPX_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HHV9058721.1"
                     /translation="KTAPFSALLSVNGERHSQKSLAEWIEDWADYLVGFDANGDAIQA
                     TKAAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLK
                     ERPFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA
                     "
     gene            612..1136
                     /gene="yfdR"
                     /locus_tag="ACUQPX_004276"
     CDS             612..1136
                     /gene="yfdR"
                     /locus_tag="ACUQPX_004276"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HHV9058722.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPTPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPTVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPGHA
                     YGMFMERFKELTES"
     gene            1245..2111
                     /locus_tag="ACUQPX_004277"
     CDS             1245..2111
                     /locus_tag="ACUQPX_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058723.1"
                     /translation="MNQNPFSFYDFLGYLIPGGFFILLMYFCGLTFDLDIVIDLTELL
                     RGQSQIFGILNYGSIVIISYIAGHFISITSAFFIEKYMNEELQYPSEYLFNKLSDDKK
                     NVLTPSSDKQNEDTKRKIKYCIIKVMLFPIIPWDYATQKLCYSQSLPFQLANTTWLMI
                     KEGYEKKFRIDDKLLNIRTGLHDDLFRLAYHYVYEFSNQHQTKIQNYVALYGFCRNIC
                     LVFVITFWISFLTLIYRAIEGGAVSYISISTLLSFFFVYVFYVGFVKFYRRYTLEVLM
                     AFAVLQSKDAIN"
     gene            2152..2439
                     /locus_tag="ACUQPX_004278"
     CDS             2152..2439
                     /locus_tag="ACUQPX_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HHV9058724.1"
                     /translation="MNTLFLLMAEFNTSNIELSAVSQKYFGMSPATAEAKAKANACKL
                     PVPTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTGKQKGKEHH"
BASE COUNT          864 a          544 c          551 g          808 t
ORIGIN      
        1 aaagacagca ccgttctctg ctctgttgtc tgttaatggc gagcgtcatt cccagaagtc
       61 actggcagaa tggattgaag actgggccga ctaccttgtg ggctttgatg ctaatggtga
      121 cgctattcag gcaacaaaag cggctgcggc tgtccgtaaa atcacgattg aagcaaacca
      181 gaccgctgat tttgaagata atgacttcag cggcaaacgc tccctgatgg agtctgtcga
      241 agcgaaaacc aaagatatta tgccagtggc atttgaattt aaatgcgttc cgtttgaagg
      301 tctgaaagaa cgtccattta aattacgcct cagcattatc actggcgatc gtcctgtact
      361 ggttctgcgc attattcagc tggaggcggt gcaggaagaa atggctaacg aatttcgtga
      421 tctgcttgtt gagaaattca aagacagcaa agtagaaacc tttattggta ctttcaccgc
      481 ctgatttcat tactgcaaat gcccctgcgg gggcatttat ggaaacgtaa ttgactcaat
      541 aatcgccgga tggtgagggc ttccttttac cagaattcag cgtggtgcag cacatatacg
      601 cggagaacaa aatgtcattt attaaaactt tttccgggaa gcatttttat tatgacagga
      661 taaataaaga cgacatcgtg attaacgata tcgcggtttc cctttcaaat atctgtcgct
      721 ttgccggtca tctttcacac ttctacagtg tcgcccaaca tgcggtgctt tgcagccagc
      781 tggtgccgca ggaatttgct tttgaagcgt taatgcatga tgcaacagaa gcgtattgcc
      841 aggacatccc cacgccgctg aaacgccttc ttcctgacta taaacggatg gaagaaaaaa
      901 tagacgccgt aatccgtgag aaatacgggt tacccacggt tatgagcacg cctgtgaaat
      961 atgccgatct catcatgctg gcaaccgaac gccgcgatct cgggcttgat gatggctctt
     1021 tctggcctgt actggaaggt atcccggcaa cagagatgtt caaagttatt ccactgtcgc
     1081 caggccatgc ctatgggatg tttatggaac gctttaaaga gttaactgag tcgtaaaaat
     1141 cagcacgtac gaattcaaac tctgccataa aagaacatat aagtaattta ttaacatata
     1201 gatataggtt atattacaaa ttgaaaaatt attggagagc aacaatgaat caaaatccat
     1261 tctcattcta tgactttctt ggatatttaa taccaggtgg tttttttatc ctattaatgt
     1321 atttctgtgg tttgacattc gatctagata ttgttattga cttaactgaa ttgctcagag
     1381 gtcaaagtca aatctttggc attttgaact atggttcaat agtaatcata tcttacatag
     1441 ctggacactt tatatctatc acatccgcat tttttattga aaaatatatg aacgaagaat
     1501 tgcaatatcc gtcagaatat ctatttaata agttatcaga tgacaaaaaa aacgtcttaa
     1561 caccatcaag tgacaaacag aacgaagaca caaagagaaa aataaaatac tgcataatca
     1621 aagtaatgtt attcccgata ataccatggg attacgccac tcaaaaacta tgttattcac
     1681 aatcattgcc atttcaatta gctaatacca catggctaat gatcaaagaa ggttatgaaa
     1741 aaaaattcag aatagacgat aaattattaa acatcaggac aggtcttcat gatgatcttt
     1801 tcagattggc ttatcattac gtctatgaat tttctaatca acatcaaaca aaaattcaaa
     1861 actatgttgc attatatggt ttttgcagaa atatttgttt agtatttgta attacatttt
     1921 ggatatcatt tctaacatta atttatcgtg ctatagaagg tggggcggtg tcttatattt
     1981 ctatttcgac gctacttagc tttttcttcg tctatgtttt ttatgttgga ttcgttaagt
     2041 tttatagacg atatacttta gaggtattaa tggcatttgc tgtacttcag agcaaagatg
     2101 ctattaatta atatattacc ctcgggtgca gccgggataa tggagaaatt tatgaacacc
     2161 ttgtttttac tgatggctga attcaatacc tcaaacattg aactctcagc agttagccaa
     2221 aagtactttg gtatgagtcc agccacagca gaagcaaaag caaaagcaaa cgcttgtaag
     2281 ttgcccgttc caacatatcg catcggcaca tcacaaaaag caaaacgttg catcaatatt
     2341 caggatcttg cggaatacat agacaaaaga cgagaagaag gacgtatcga gtgggaacag
     2401 gtcagaacag gcaaacagaa gggcaaagaa catcactaaa gaaaaaaccc gcctaaaggc
     2461 gggttttcaa aaagcaccag ctatgatcat gctgctttgc gacgacgaag cttaccctgc
     2521 tgctctttac cagagacagt agcgtgagtg aacgcattag gagcagcctt catcagaact
     2581 tcaaagcagc ccccatacct gcgaatgctt tcattgtgtc gaacttaagc tgtggcttgg
     2641 ttgctttttg atcttccata gaaaactcca gaagttatac cgaaacaatt cctgttgttt
     2701 actcatcatc aatagatgat acgcaatatt tatttttaaa tttaaggttc tttggcgtaa
     2761 cttcatc
//
LOCUS       DBKEKI010000257         2734 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.260, whole genome shotgun sequence.
ACCESSION   DBKEKI010000257 DBKEKI010000000
VERSION     DBKEKI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.260"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1682)
                     /locus_tag="ACUQPX_004279"
     CDS             complement(<1..1682)
                     /locus_tag="ACUQPX_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HHV9058725.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVVIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENA
                     TAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIA
                     LGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVG
                     NSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTY
                     NLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSK
                     DAVNGSQLKATNDDVEANTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKI
                     TNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT"
     gene            complement(1726..2409)
                     /locus_tag="ACUQPX_004280"
     CDS             complement(1726..2409)
                     /locus_tag="ACUQPX_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HHV9058726.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKNLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
BASE COUNT          679 a          611 c          583 g          861 t
ORIGIN      
        1 gtcaggttag agatgttggt ggtgttagtg gcaatattgg aagtgttatt ggcgatattg
       61 gtggtattcg tcgccacagc atcgttggtg gttttcagct gagagccgtt aacagcatca
      121 gtgctgtcag ccgtcaggtc ggcatcttta acgttggtga ttttgctggt ggtatcctgg
      181 ccgtgagctg cactgaatgc ctttttagtt tcgttccaga gcagggcatc agcctgaagg
      241 tcaccaacgg aatccgtcag gttggtgata ttggtggtgt tggcttcgac atcgtcattg
      301 gtagctttca gttgggaacc gttaaccgca tccttactgg aggctgaaat cgtgccatcc
      361 gcaacatcgg tgattacgct ggcagttggg ctactagtac catgagcagc gctgtatttg
      421 cctttggttt ggtcccattg cagggtgttt tcatcaagta cagcgagcgc agcccctacg
      481 ttatttttgc taccattttt taaattgtag gttggtgctg taacagtacc gtcatcaaca
      541 tctactgcag cccctcctcc aagccttttt gctaccgagt cgctaatggc ataaagctgt
      601 gaaccattaa tggcatcgta actgtcagac ttgatcgcgc catttttcac attaacgatc
      661 ttgcgtttta atgaactatt gccgacagag acggtattgt ctaaatccgc aatggaatct
      721 gcgcccagcg cgacagagtt aataccgttg gcactgctat tactaccaag cgcaagactc
      781 ttatcaccag ttgcattact ggctgtgcct aaggcaatgg tgtttaagcc tctcgactgg
      841 ctaccattac ccagagcgat ggcatccacg ccatcagctt tgttgccctg gcctatggcg
      901 attgcattat ctgccagagc ctggctgcca tttcctaatg cgatagtatt gactttatcg
      961 gcaaggcttc cgaaccccat tgccatgcta ttagtccctt ttgccttcgc attattgcca
     1021 atagcagttg cattttcagc ggcttcggtc tcagcaccta tcgcaatagc gttcgctgcg
     1081 ctggcaagtg attgtctgcc aaatgcgagg ctgtcagtac cgctagctac actgctacgc
     1141 cccagggcaa tggcgtttgc tccttccgct ttggaagagt cccccatagc aatagacaat
     1201 ttacccaatg cttttgaata acggccgagg gccattgagt attggcccaa tgaatacgaa
     1261 gaggcaccca gtgctatact tctatcgcct tctgatattg cactaacccc aaaggccatt
     1321 gatgcaccac caatcgcatg ggtttttgac ccgatggcag agctatattg gccttcagct
     1381 atagcgtcat aacccacagc agtactggaa ccactggtgt tcataaaagt atttgctttc
     1441 gcccctttgc ctataacaac ccagccgtcg ccagctgatc cactaccgta atcaacaccc
     1501 tgaccgttgt cattcccggc attcgccaat gccccaaacg acgacaacat tccacccgca
     1561 accagcgcag aaattaacag cttactgcgc ccagatttct tgccacggct ttttgccgtt
     1621 tcgctggtaa cagtataatt ccctgtcgca gggttccaga taactttaaa tattttgttc
     1681 attaatgatg ctcgctattc cttgttataa gcgtactaac tgcatttatt ttttgcttct
     1741 tttctgccga ttttcagcag agggaagatt gtcgaactga atattttcca tgttgatggc
     1801 taacttcaga tatcccaggt tattgggcga tacacccttc agggtcaaat taatgtcttg
     1861 ccattgacct ggtaataatt tcaatgtgga tttcattgat gtgttgaaaa gtaattgttg
     1921 ccaccgggca aactctttgg catcgacagg tttttcacca ccccatgtcg cattaaaacc
     1981 aatgtttgag ataggtgtac ttaataagtt gaccagtgag atatgaataa cactaccatt
     2041 ctctgtggcg tcaatggata acactcgacc cgctaaagcc accgaaccgt tgttcaggat
     2101 gaaatacttc gtttttgctg ggttaaactc ggtaaaaata aaagtatttt gttggtcttt
     2161 aacggattta attacgtgag ttaaattctt tgtactgttt attatgccag tttgttgttt
     2221 ttgaatagtt tcgagttgac tctttagctg tgcttgttgt ttttcaagta gcacaatttt
     2281 attattatgt tcttcttgtt gtttcagtaa actactttct ggcttgcctt cttcaatgta
     2341 gtcacatccc gttaatgcgc agagaactga aacagtgaca gcactgaaaa aaatcttctt
     2401 caagttcatg taaaaatcct tatttcctcc gaccagcgat caatgccggt agagttgtcc
     2461 attgtattat tggtgagcta tgttgcttta cattaatgac agtgatgtgt gtcgattaaa
     2521 tattgtttta agcattcatc ataaaaatgt atcattcgtt tcaaaagatg tctgtgtttg
     2581 tatgaatata ttatttttcg tggattctaa tgatgattat taataaatgt caaatatgaa
     2641 agaaaccatg tctgggaaaa attttacaga taattgattt tgtgaatatg atgattaaaa
     2701 tttcacaatt aaatccgaaa aaaagagacg taaa
//
LOCUS       DBKEKI010000258         2702 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.261, whole genome shotgun sequence.
ACCESSION   DBKEKI010000258 DBKEKI010000000
VERSION     DBKEKI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.261"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..294
                     /locus_tag="ACUQPX_004281"
     CDS             <1..294
                     /locus_tag="ACUQPX_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HHV9058727.1"
                     /translation="HRPRSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHL
                     RPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            534..794
                     /locus_tag="ACUQPX_004282"
     CDS             534..794
                     /locus_tag="ACUQPX_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HHV9058728.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1018..1092
                     /gene="tap"
                     /locus_tag="ACUQPX_004283"
     CDS             1018..1092
                     /gene="tap"
                     /locus_tag="ACUQPX_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HHV9058729.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1085..1942
                     /gene="repA"
                     /locus_tag="ACUQPX_004284"
     CDS             1085..1942
                     /gene="repA"
                     /locus_tag="ACUQPX_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HHV9058730.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YNRLATASP"
BASE COUNT          693 a          706 c          690 g          613 t
ORIGIN      
        1 caccggccac gatcacgcca gtggtggcag ttattcagga tggtgtctca gtggcatgtt
       61 gatgtggtta tcgttgagct gcgttcgttc agtattgttg cagcagtaga gctggatgat
      121 gccagccatt tacgaccgga acgcagacgc cgggatattc ttctggaaga ggttctgagg
      181 caggctggta ttccgttgct cagaagccac gatgccagaa aactgctgca gatgaccgga
      241 gaatggctga atacaacagg ggctgatcag cagtcaccgg aacatcgtag ctgacgcctt
      301 cgcgttgctc agttgtccaa ccccggaaac gggaaaaagc aagttttcct cgctcccggc
      361 gtttccataa ctgaaaacca tactatttca cagtttaaat cacattaaac gacagtaatc
      421 cccgttgatt tgtgcgccaa cacagctctt cgccacaatt ctcaagtcgc tgatttcaaa
      481 aaactgtagt atcctctgcg aaacgatccc tgtttgagta ttgaggaggc gagatgtcgc
      541 agacagaaaa tgcagtgact tcctcattga gtcaaaagcg gtttgtgcgc agaggtaagc
      601 ctatgactga ctctgagaaa caaatggccg ctgttgcaag aaaacgtctt acacacaaag
      661 agataaaagt ttttgtcaaa aatcctctga aagatctcat ggttgagtac tgcgagagag
      721 aggggataac acaggctcag ttcgttgaga aaatcatcaa agatgaactg cagagactgg
      781 atatactaaa gtaaagactt tactttgtgg cgtagcatgc tagattactg atcgtttaag
      841 gaattttatg gctggccacg ccataaggtg gcagggaact ggttctgatg tggatgtaca
      901 ggagccagaa aagcaaaaac cccgataatc ttctttaact ttggcgagtc agaaagatta
      961 ccggggccca cttaaaccgt atagccaaca atcaagctat gcggggagta tagttatatg
     1021 cccggaaaag ttcaagactt ctttctgtgc tcgctccttc tgcgcattgt aagtgcagga
     1081 tggtgtgact gatcttcaac aaacttatta ccgccaggta aagaacccga atccggtgtt
     1141 cacaccccgt gaaggtgccc ggacgcttcc gttctgcggg aaactgatgg aaaaggcggt
     1201 gggcttcacc tcccgttttg atttcgccat tcatgtggca catgcccgtt cgcttggtct
     1261 gcgtcggcgc atgccaccgg tactacgtcg ccgggccatt gatgcgctgc tgcaggggct
     1321 gtgttttcac tatgacccac tggccaaccg cgtccagtgt tccatcacca cgctggccat
     1381 tgagtgcgga ctggcgacgg agtctgctgc cggaaaactc tccatcaccc gggccacccg
     1441 ggccctgacg ttccttgcag agctgggact gattacctac cagacggaat atgacccgct
     1501 tatcgggtgc tacattccga ccgatatcac gttcacatct gcactgtttg ctgccctcga
     1561 tgtatcagag gaggcagtgg ccgccgcgcg ccgcagtcgt gtggaatggg aaaacagaca
     1621 gcgcaaaaag caggggctgg ataccctggg tatggatgaa ctgatggcga aagcctggcg
     1681 ttttgttcgt gagcgtttcc gcagttacca gacagagctt aagtcccgtg gaatgaagcg
     1741 tgcccgtgcg cgtcgtgatg cggacaggca gcgtcaggat attgtcaccc tggtgaaacg
     1801 gcaactgacg cgtgaaatct cggaagggcg cttcactgcc agtcgtgagg cggtaaaacg
     1861 tgaagtggag cgtcgtgtga aggagcgcat gattctgtca cgtaaccgta attacaaccg
     1921 gctggccaca gcttccccct gaaagtgacc tcctcagaat aatccggcac gcgccggagg
     1981 cattcgcacg cctgaagccc gccagtgcac aaaaaatcag cgctacatac aaaaaacaac
     2041 ctcatcatcc accttctggt gcatccggtc ccccctgttt ttgatacaaa acacgcctca
     2101 cagacgggga attttgctta tccacattaa actgcaaggg acttcaccat aaggttacaa
     2161 ccgttcacgt cataaagcgc cagccgtcag ccctacaggg tgcgatgtat cttttaaaca
     2221 cctgtttata tctcctttaa actacttaat tacattcatt taaaaagaaa acctgttcac
     2281 tgcctgtcct ttggacagac agatatgcac ctcccaccgc aagcggcggg ccccgaccga
     2341 agctggttta gttacaacat gcaaacataa acccctaaaa aacccaagtc cgacgcagaa
     2401 ctgaaaccac aacgcccctc ctcataactg aaaagcgccg ccgcccccgc ccgaagggcg
     2461 ggaacagagt cgcttttaat tatgaatgtt gtaactacat catcatcgct gtcagtcttc
     2521 tggctagacg tatcgagtac acgctcgtaa gcggccctca cggcccgcta acgcggagat
     2581 acgccccgac ttcgggtaaa ccctagtcgg gaccactccg accgcgcaca gaagctctgt
     2641 catggctgaa aacgggtatg gcttggcagg gctgggggtg ggtaaggtga aatctgtcaa
     2701 tc
//
LOCUS       DBKEKI010000259         2679 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.262, whole genome shotgun sequence.
ACCESSION   DBKEKI010000259 DBKEKI010000000
VERSION     DBKEKI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.262"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            140..1321
                     /gene="nimT"
                     /locus_tag="ACUQPX_004285"
     CDS             140..1321
                     /gene="nimT"
                     /locus_tag="ACUQPX_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HHV9058731.1"
                     /translation="MTCSTSLSGKNRIVLIAGILIIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            1376..1723
                     /locus_tag="ACUQPX_004286"
     CDS             1376..1723
                     /locus_tag="ACUQPX_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HHV9058732.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(1745..1999)
                     /gene="yoaF"
                     /locus_tag="ACUQPX_004287"
     CDS             complement(1745..1999)
                     /gene="yoaF"
                     /locus_tag="ACUQPX_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HHV9058733.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            2182..2679
                     /gene="dgcP"
                     /locus_tag="ACUQPX_004288"
                     /pseudo
     CDS             2182..2679
                     /gene="dgcP"
                     /locus_tag="ACUQPX_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
BASE COUNT          614 a          680 c          669 g          716 t
ORIGIN      
        1 gtcggtcggg ttcatagcca ttgagattca acctgtgcat cattttgtcc gaacttagcg
       61 ataatttgtc attttagctt gattcaacat aacaataaaa acggtaaggt acagcctcgt
      121 ttgtaacaat gagaagcata tgacctgttc aacttcatta agcggcaaaa acaggattgt
      181 ccttatcgct ggcattctga taattgccac aacattacgc gtcaccttta ccggcgcagc
      241 gccgttactg gatacgattc gttccgctta ctcgctgacg acagcgcaaa ccggcttatt
      301 aaccaccctg ccattattgg cctttgcgct aatctcacct ctggctgccc cggtggcgcg
      361 acgttttggt atggaacgta gcctgtttgc cgcgttactt ttgatctgtg caggtatcgc
      421 aattcgttct ctcccttcgc cttacttatt atttggcggt acagcggtca ttggcggtgg
      481 gattgcatta ggcaatgtct tactgccagg attaattaaa cgcgatttcc ctcattccgt
      541 cgccagactt accggcgcat attccctgac aatgggagct gcagcggcac tgggatcggc
      601 tatggtcgtg ccgctggctt tgaacggttt tggctggcaa ggcgcgttgt tcatgctgat
      661 gtgttttcct ctgctggctc tttttttatg gctgccacag tggcgaagtc agcaacatgc
      721 aaatttgagt acctcacgcg ccttacatac tcggggtatc tggcgttcgc cacttgcctg
      781 gcaggtaaca ttgtttcttg ggatcaactc actggtctat tacgtgatta ttggctggct
      841 tccggcgatc ctcatcagtc acggctatag cgaagcacag gcgggttcac tgcatggttt
      901 gctgcaacta gccacagcag ctcccggttt gctgatccca cttttcttac atcatgtgaa
      961 agaccagcgt ggtattgcag cgttcgttgc cttgatgtgc gcagtgggcg cggttgggct
     1021 ctgctttatg ccagcgcacg cgatcacctg gactctgctt ttcggttttg gttccggcgc
     1081 aacaatgata ctggggttga cgttcattgg tcttcgggca agttctgcgc atcaggcggc
     1141 ggcactctcg gggatggcac aatccgtcgg gtatttgctg gcagcctgtg ggccgccgct
     1201 aatgggtaaa atacacgatg ctaacggtaa ctggtccgta ccactgatgg gtgttgcaat
     1261 actttcacta ctgatggcga ttttcggact ttgcgccggg agagacaaag aaattcgcta
     1321 atatccggtg ctatagtgac gtgacaaatc ttgcgtgaaa gggagaacaa acacgatgaa
     1381 tattcagtgc aaacgcgttt atgatccggc tgaacagagc gatggttatc gcgtactggt
     1441 cgaccgcctc tggccgcgcg gtatcaaaaa aaccgattta gcccttgatg agtgggataa
     1501 agaaatcacg ccgtcaacgg aactgcgcaa agcctttcac ggcgaagtcg tcgattttgc
     1561 aacctttcgc gagcaatatc gcgcagaact ggcgcaacac gagcaagaag gaaagcggct
     1621 ggcggacatc gccaaaaaac agccgctgac cctgctctac gcagcaaaaa acaccacgca
     1681 gaaccatgcg ctggtgctgg ccgactggct acgtagcttg tgattttagt acggcatccg
     1741 gcggttattt ttcaccagcc ggatggtccc gtcgccacaa tgcccattca tcaatcgttt
     1801 caccgcccgg taatttgcaa ttgttgctga ccccttgcgc tgtctgcact ggaatgagcg
     1861 tcccgccctt ctgctggcaa tagaccgacg ccggatttgc cataccaatc tgcggcggtt
     1921 taggtgcttc tggctgagaa ggggttgaac aaccagccag gaccagcaag caaggcagaa
     1981 caaaactgat aattttcatt tattgatctc acatatttat ccaagcttag agtatcgcgg
     2041 tatcgttttg ttttgcagca ctatttttat tacattcact caaaacatat tacgtcttgt
     2101 ttcatctttg ttgatgatgt tttatcatgc ctgcaaagat taaataatca gcacttaccc
     2161 gccgtatcct ggtgttgttc cgtgtcagat cagattatcg cccgcgtctc gcaatccctt
     2221 gccaaagaac agtcactgga aagtctggtc cgacagcttc tggagatgct ggaaatggtc
     2281 actgatatgg aatcaaccta cctgaccaaa gtggatgtcg aagcgcgcct gcagcatatt
     2341 atgtttgccc gtaacagcca gaaaatgcac atcccggaga attttaccgt ctcgtgggat
     2401 tactcgttat gcaaacgcgc cattgatgaa aactgctttt tcagcgatga agtccctgac
     2461 cgttggggcg actgtattgc ggcacgcaat cttggcatca ccacatttct gagcacgcca
     2521 attcacttac cggatggatc attctatggc acgctttgcg ccgccagcag tgagaagcgc
     2581 cagtgaagtg aacgcgcgga acaggtttta cagttattcg ccggactgat tgcacaatat
     2641 attcaaaaag aggcactggt tgaacagctg cacgaagcc
//
LOCUS       DBKEKI010000260         2665 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.263, whole genome shotgun sequence.
ACCESSION   DBKEKI010000260 DBKEKI010000000
VERSION     DBKEKI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.263"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..428)
                     /locus_tag="ACUQPX_004289"
     CDS             complement(<1..428)
                     /locus_tag="ACUQPX_004289"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HHV9058734.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGA"
     gene            complement(525..1415)
                     /gene="garR"
                     /locus_tag="ACUQPX_004290"
     CDS             complement(525..1415)
                     /gene="garR"
                     /locus_tag="ACUQPX_004290"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HHV9058735.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADCNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(1445..2215)
                     /gene="garL"
                     /locus_tag="ACUQPX_004291"
     CDS             complement(1445..2215)
                     /gene="garL"
                     /locus_tag="ACUQPX_004291"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HHV9058736.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(2231..>2665)
                     /gene="garP"
                     /locus_tag="ACUQPX_004292"
     CDS             complement(2231..>2665)
                     /gene="garP"
                     /locus_tag="ACUQPX_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HHV9058737.1"
                     /translation="AGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYT
                     NNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVI
                     GYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT          617 a          774 c          641 g          633 t
ORIGIN      
        1 gcgccgccat catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc
       61 tccagcgcct gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt
      121 ttttccgcag gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg
      181 ccatcgccgg agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag
      241 gcgtgacgtt cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg
      301 tcggcaaccg gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct
      361 atcgcctgcg caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg
      421 attttcatac ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg
      481 gattccaggc ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg
      541 actttcgcca gtttttcgta gtagcacgcc agggcgctat gatccgccgt tcctaaacca
      601 tctgctcgca gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgcca
      661 acgccgtggg aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag
      721 cccggcttga agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc
      781 gccagtccac cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc
      841 agcgttaacg cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt
      901 ttggtgacgt tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc
      961 atcaaatcat agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta
     1021 ccgtcgatgg ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc
     1081 gctttcagcg cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc
     1141 aataccgtac ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc
     1201 acatgagggg agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta
     1261 gccgtagagg ctgtttctgc acctgcagca atcacatcag caatagcttc tgggttacag
     1321 tcagcaacca ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata
     1381 atccccaggc caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg
     1441 gtggttattt tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat
     1501 cgctgccgac agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga
     1561 ccggcgcgag gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa
     1621 ttgctttttg tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat
     1681 cgctggggcc gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat
     1741 ctacgccctg ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga
     1801 aataatccgc cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc
     1861 cttccggtgg gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa
     1921 aaggaatcag gaagttatag aaaccgatat ccagaagacg cttaataatt accggctcgt
     1981 tagtcggcac tcgcactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa
     2041 taaacgtgga gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag
     2101 ccaaaccaag aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta
     2161 cctgtttcgc agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt
     2221 atacctttgc ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc
     2281 agaccatcgc catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca
     2341 gttcacttac caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga
     2401 cgttaaagac gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg
     2461 gccagcccag agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca
     2521 gcgtggtgtt gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca
     2581 caatcggtag cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga
     2641 agacacctcc cagcacgccg cccgc
//
LOCUS       DBKEKI010000261         2602 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.264, whole genome shotgun sequence.
ACCESSION   DBKEKI010000261 DBKEKI010000000
VERSION     DBKEKI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.264"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..555
                     /locus_tag="ACUQPX_004293"
     CDS             <1..555
                     /locus_tag="ACUQPX_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9058738.1"
                     /translation="DLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELL
                     MCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSER
                     KVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGAREL
                     NLPSVYGDAAEWNTAELRREMSHS"
     gene            513..1190
                     /gene="bglJ"
                     /locus_tag="ACUQPX_004294"
     CDS             513..1190
                     /gene="bglJ"
                     /locus_tag="ACUQPX_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HHV9058739.1"
                     /translation="MEHSRIKKRNVALIEKCIMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1228..2016)
                     /gene="fhuF"
                     /locus_tag="ACUQPX_004295"
     CDS             complement(1228..2016)
                     /gene="fhuF"
                     /locus_tag="ACUQPX_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HHV9058740.1"
                     /translation="MTYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2157..2393
                     /locus_tag="ACUQPX_004296"
     CDS             2157..2393
                     /locus_tag="ACUQPX_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HHV9058741.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2432..2518)
                     /locus_tag="ACUQPX_004297"
     tRNA            complement(2432..2518)
                     /locus_tag="ACUQPX_004297"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2482..2484),aa:Leu,seq:cag)
BASE COUNT          667 a          584 c          665 g          686 t
ORIGIN      
        1 gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta ttctttaatc
       61 tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta ttcccaggct
      121 gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc cattgagaat
      181 ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc catgctgacc
      241 tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct cactctttca
      301 gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat agcttcattg
      361 cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg tcgactggca
      421 attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc aagagaactt
      481 aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt aagaagagaa
      541 atgtcgcact catagaaaaa tgcatcatga gtagtatcgg tattgagagt ttattcagaa
      601 agtttgcggg caacccttat aagctccata cctataccag tcaggagtca tttcaggatg
      661 ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg agaagtgagc
      721 gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt acccggcgtt
      781 tagttattgc ggatgatgat attgaagctc ggctgattgg ttcattgtcg ccatcaccgc
      841 tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa ctctttttgt
      901 cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac attaaccaaa
      961 gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt ggctactcaa
     1021 tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg cataaattta
     1081 atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc gcagatattc
     1141 tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa tccgcatgat
     1201 gccggtttta cttccccggc agtgctttca tttcagcgta caatagccac attgctgcac
     1261 atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt cgcgcagtac
     1321 cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa aaaagagggc
     1381 atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag tgagatacca
     1441 gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc cggtcgcttc
     1501 tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc gctgctgcgg
     1561 cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg cgacgcgtcc
     1621 ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt tttcctgcgt
     1681 cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg cccatagtga
     1741 aatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat gatcggaata
     1801 gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg tcatggcgtt
     1861 aagcggagcg ggttcatcca agcggataaa ctccagcaaa tgttcacgat gtttggcgat
     1921 catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg ttcgccagat
     1981 gacatcttca tagagcggtg cggaacgata ggtcataatc gtgatagtaa tctaaatgat
     2041 aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc tggcttgcaa
     2101 atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat gaaaccatgt
     2161 tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg attgcagggc
     2221 tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg ggattagtat
     2281 tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta ccatcgtgcc
     2341 tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga tgacaaaaca
     2401 gggaagaaac aagaaaggaa gtaaagataa ttggtgcgag gggggggact tgaaccccca
     2461 cgtccgtaag gacactaaca cctgaagcta gcgcgtctac caattccgcc accttcgcac
     2521 agtcatctta ctttttttga tatcgcctcg tttggtgcga ggggggggac ttgaaccccc
     2581 acgtccgtaa gaacactaac ac
//
LOCUS       DBKEKI010000262         2574 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.265, whole genome shotgun sequence.
ACCESSION   DBKEKI010000262 DBKEKI010000000
VERSION     DBKEKI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.265"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            747..2255
                     /gene="ltrA"
                     /locus_tag="ACUQPX_004298"
     CDS             747..2255
                     /gene="ltrA"
                     /locus_tag="ACUQPX_004298"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HHV9058742.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            2336..2574
                     /locus_tag="ACUQPX_004299"
                     /pseudo
     CDS             2336..2574
                     /locus_tag="ACUQPX_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          641 a          643 c          766 g          524 t
ORIGIN      
        1 ccgcaggcga ccgcgtaaac cgtcagttcg tggcagaacg acctgaccag ctgtgggtgg
       61 ctgattttac ttacggtgtg cccggagttc agggcgggca tggatgctta aatgaaccgc
      121 gagtctgtct ggaatattga accggtaact cacgatgaga aacccaacaa tcccaccggg
      181 tgtgacggtg gagaacctga gcggcagtga cctgcagcat gcccgcaggg tgatgtaacc
      241 cgctgacaac ggggattgag gcgagatcac taagccgaga tgatcctcaa ggttaagtac
      301 tgaaaggctg aagaacatga acccgttaat ccgcctctgt gggttgaaaa cgtcaccacg
      361 gcctacgtga tctgacaggc cgtgcaggag gaactggcag tgatacgtaa gcactgccgg
      421 tcgaaggtgt tttgacatgt atgcgaaaca ccggggcagc agcgtctatc acgcttgcgt
      481 tgctgacttc tgccaacttg cggcaagcaa ggataaagag tgcgacgggc agcctcctca
      541 gtatgcctga gtccaggcag gtaaaccggg gaaggtcagc gacggatgtt aagggggcat
      601 ggctccgatg acgcgctggc tggcggagct tccgtagtag tccgcgatgg ggaaagccca
      661 ttacatggcg aagggaagca gtttgaatgt gtttgcgacg tgaattaact gacctaacga
      721 ggtgaagacc tttgataatc agcgaaatgc aacgcaagct tgccacatgg gcagccaccg
      781 atccgtccct acggattcaa cggctgctgc gtctgataac acaaccagaa tggctggctg
      841 aagcggcgcg gatcacgctt tcatcaaagg gggcgcatac ccccggcgtt gatggcgtga
      901 acaaaacaat gctacaggcc agactggctg ttgagctgca aatcctcagg gatgaattac
      961 tctcaggcca ctaccagccc ttgcccgcca gacgggttta catccctaaa agcaacggca
     1021 aactgcgacc actgggtatc cccgcgttgc gggatcgtat tgttcagcgg gccatgctga
     1081 tggcgatgga gccgatatgg gagagtgatt ttcatacgct ctcatatggc ttccggcctg
     1141 agcgcagtgt ccaccacgcg atccgcacgg tgaaattaca gctcacagac tgcggtgaaa
     1201 cccggggacg ctgggtgatt gaaggcgacc tgtccagtta cttcgacacc gtacatcatc
     1261 gactgctgat gaaggctgta cgccgcagga tcagtgacgc acgtttcatg actctgctgt
     1321 ggaaaaccat caaggcggga catatcgatg tcggtctctt tcgggcggcc agtgaaggtg
     1381 tgccacaggg cggtgttata tcgccgctat tatcgaacat catgctgaat gagttcgatc
     1441 aatacctgca tgagcgctac ctgagcggga aagccagaaa agatcggtgg tactggaata
     1501 acagtatcca acggggccga agtacggcgg tcagagaaaa ctggcagtgg aaacccgcgg
     1561 tggcgtactg ccgctatgcc gatgattttg tcctcatcgt caaaggcacc aaagcacagg
     1621 cggaagccat cagggaggag tgtcggggtg tgctcgaagg cagtctgaaa ctcaggctga
     1681 acatggataa gactaaaatc acccatgtta atgacggctt tatctttctg gggcacagga
     1741 tcattcgcaa acgcagtcgt tatggcgaga tgcgagtggt ctcaacgatc ccgcaggaga
     1801 aagccagaaa cttcgccgca tcgctgacag cactgttatc aggcaactac agtgaaagca
     1861 aagtcgatat ggctgaacaa ctcaaccgaa aactgaaagg ctgggccatg ttctatcagt
     1921 tcgttgattt taaggccaaa gtcttcagtt atatcgaccg tgtcgtgttc tggaagctgg
     1981 ctcactggct ggcccgcaaa taccgtacag gtatcgcttc cctgatgagg tggtggtgta
     2041 aatcaccgaa accgggtcag agcaaaacgt gggttttatt tggtaaaacc aatcacggca
     2101 agctcagcgg cgaaatactg taccggttgg tggggcaagg caagaagctg ttccgctggc
     2161 ggctacccga aggtaatccc tatctgagga cggagaccag aaacacgtat acatcgcgct
     2221 ttacagaagt ggcaatggcg ttcgccagca tttaaatgga gagccggatg cgctgaaagg
     2281 tgcacgtccg gttcggggag gagaggcagg gaaatagtcc gactacgccc tgcctcttac
     2341 tctactctgg acgggtaagc gctgggcgta cctcgccgtt gttcccgacc tgttcgcaag
     2401 aaaaccagtg ggctgggcca tgtcgttctc gccggacagc aggctcacca tgaaagcgct
     2461 ggaaatggca tgggaaaccc gtggtaaacc cggcggggtg atgttccaca gcgatcaggg
     2521 tagtcattat acgagcaggc agttccggca gttattgtgg cgataccaga tcac
//
LOCUS       DBKEKI010000263         2563 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.266, whole genome shotgun sequence.
ACCESSION   DBKEKI010000263 DBKEKI010000000
VERSION     DBKEKI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.266"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            312..515
                     /locus_tag="ACUQPX_004300"
     CDS             312..515
                     /locus_tag="ACUQPX_004300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058743.1"
                     /translation="MTDPLKALFGKPDYSHIVRDTTATISITAAEMAAVLEAYDRGID
                     TLDGTTRTALDSVISKLKDEVWP"
     gene            506..727
                     /locus_tag="ACUQPX_004301"
     CDS             506..727
                     /locus_tag="ACUQPX_004301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058744.1"
                     /translation="MAMSMYYIKTGLEYWKPKGYGYTKSREEAGVFTLKDMEELNLDG
                     CTLERAEVAFLDEDGPSPDLPHRVTLPRD"
     gene            complement(962..1321)
                     /locus_tag="ACUQPX_004302"
     CDS             complement(962..1321)
                     /locus_tag="ACUQPX_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization domain protein"
                     /protein_id="HHV9058745.1"
                     /translation="MSLAEKVFGAVETVEGVAQRLNAKIMGSVQPMAEKAAVSAQNER
                     RAKELRETLAQQQKRLQALQEPFKGLSKDQVAGLIRQAVKLRQENEQEKQERAQQIKE
                     RFKAKRERERSDRSRGR"
     gene            complement(1478..2338)
                     /locus_tag="ACUQPX_004303"
     CDS             complement(1478..2338)
                     /locus_tag="ACUQPX_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HHV9058746.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          639 a          637 c          627 g          660 t
ORIGIN      
        1 gagcatgagt atcatacgga tgctcgggtc gaaagggaat ccccaggcga gtaacctgtt
       61 tgcggtgatc cattagctgc aggagcagaa gagcatacat ctggaagcaa agccaggaaa
      121 gcggcctatg gagctgtgcg gcagcgctca gtaggcaatt tttcaaaata ttgttaagcc
      181 ttttctgagc atggtatttt tcatggtatt accaattagc aggaaaataa gccattgaat
      241 ataaaagata aaaatgtctt gtttacaata gagtgggata cagacacgct tgatatgttt
      301 ggagatcagc catgacagat ccactcaaag cccttttcgg caagcctgat tactcccata
      361 tcgtaaggga caccacggcc actatcagca tcacagccgc tgaaatggcc gctgttttgg
      421 aggcttacga caggggtata gacacgctgg acggcaccac cagaacggcg ctggattccg
      481 ttatatcgaa actcaaggac gaggtatggc catgagcatg tattacatca agacaggtct
      541 ggagtactgg aaacccaagg gctacgggta caccaagagc cgcgaggagg cgggggtttt
      601 caccctcaag gacatggaag aattgaacct cgatggctgc accctggaac gcgccgaagt
      661 ggcgttcctg gacgaggacg gccccagtcc cgacctaccc caccgcgtca ccctgccccg
      721 cgattgagcg gggcgctgga gggtttcaaa ggggcaccct ttggcacggg atatgtgtca
      781 aaaacggagt ttttgtaaac atatccgacg tgcctgtaaa tccgcgcgat tactgccagc
      841 ccttacaccc cggttgctta gggttggttg aaagtggttt agggttggtt gaaagtggtt
      901 taatgttggt taaacaatgg tttaaggttg ttcaaggttg gttaaagttg gttgaaaagg
      961 gttaacggcc cctgctgcgg tcgctgcgct cccgttcccg cttggccttg aatcgctctt
     1021 tgatctgctg ggcgcgctcc tgcttctcct gctcgttctc ctgccgcaat ttcaccgcct
     1081 gccggatcag tcccgctacc tgatccttgg atagcccctt aaacggctcc tgaagggcct
     1141 gtaagcgttt ttgctgctgg gctagggttt cccttagctc cttggctctt cgctcgttct
     1201 gggcgcttac agcggctttc tcggccatcg gctgcacaga ccccattatt ttggcgttca
     1261 acctttgcgc caccccttcg accgtctcca cggctccgaa caccttctcg gccagtgaca
     1321 cccccttgac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1381 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1441 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1501 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1561 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     1621 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1681 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1741 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     1801 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1861 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     1921 tcgttgtcag aagtaagttg gcagcagtgt tatcactcat ggttatggca gcactgcata
     1981 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2041 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     2101 ataataccgc accacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2161 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2221 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2281 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg aaaatgttga atactcatac
     2341 tcttcctttt tcaatattat tgaagcattt accagggtta ttgtctcatg agcggataca
     2401 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     2461 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     2521 tcacgaggcc ctttcgtctt caagaatttt ataaaccgtg gag
//
LOCUS       DBKEKI010000264         2562 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.267, whole genome shotgun sequence.
ACCESSION   DBKEKI010000264 DBKEKI010000000
VERSION     DBKEKI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.267"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..2561
                     /locus_tag="ACUQPX_004304"
     rRNA            <1..2561
                     /locus_tag="ACUQPX_004304"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          665 a          564 c          808 g          525 t
ORIGIN      
        1 ccccgtacac aaaaatgcac atgctgtgag ctcgatgagt agggcgggac acgtggtatc
       61 ctgtctgaat atggggggac catcctccaa ggctaaatac tcctgactga ccgatagtga
      121 accagtaccg tgagggaaag gcgaaaagaa ccccggcgag gggagtgaaa aagaacctga
      181 aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg tgactgcgta ccttttgtat
      241 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
      301 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      361 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      421 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      481 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      541 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      601 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      661 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      721 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      781 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacgct
      841 tatgcgttgt tgggtagggg agcgttctgt aagcctgtga aggtgtactg tgaggtatgc
      901 tggaggtatc agaagtgcga atgctgacat aagtaacgat aaagcgggtg aaaagcccgc
      961 tcgccggaag accaagggtt cctgtccaac gttaatcggg gcagggtgag tcgaccccta
     1021 aggcgaggcc gaaaggcgta gtcgatggga aacaggttaa tattcctgta cttggtgtta
     1081 ctgcgaaggg gggacggaga aggctatgtt ggccgggcga cggttgtccc ggtttaagcg
     1141 tgtaggctgg ttttccaggc aaatccggaa aatcaaggcc gaggcgtgat gacgaggcac
     1201 tacggtgctg aagcaacaaa tgccctgctt ccaggaaaag cctctaagca tcaggtaaca
     1261 tcaaatcgta ccccaaaccg acacaggtgg tcaggtagag aataccaagg cgcttgagag
     1321 aactcgggtg aaggaactag gcaaaatggt gccgtaactt cgggagaagg cacgctgata
     1381 cgtaggtgaa gcgacttgct cgtggagctg aaatcagtcg aagataccag ctggctgcaa
     1441 ctgtttatta aaaacacagc actgtgcaaa cacgaaagtg gacgtatacg gtgtgacgcc
     1501 tgcccggtgc cggaaggtta attgatgggg ttagcgcaag cgaagctctt gatcgaagcc
     1561 ccggtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa
     1621 gttccgacct gcacgaatgg cgtaatgatg gccaggctgt ctccacccga gactcagtga
     1681 aattgaactc gctgtgaaga tgcagtgtac ccgcggcaag acggaaagac cccgtgaacc
     1741 tttactatag cttgacactg aacattgagc cttgatgtgt aggataggtg ggaggctttg
     1801 aagtgtggac gccagtctgc atggagccga ccttgaaata ccacccttta atgtttgatg
     1861 ttctaacgtg gacccgtgat ccgggttgcg gacagtgtct ggtgggtagt ttgactgggg
     1921 cggtctcctc ctaaagagta acggaggagc acgaaggttg gctaatcctg gtcggacatc
     1981 aggaggttag tgcaatggca taagccagct tgactgcgag cgtgacggcg cgagcaggtg
     2041 cgaaagcagg tcatagtgat ccggtggttc tgaatggaag ggccatcgct caacggataa
     2101 aaggtactcc ggggataaca ggctgatacc gcccaagagt tcatatcgac ggcggtgttt
     2161 ggcacctcga tgtcggctca tcacatcctg gggctgaagt aggtcccaag ggtatggctg
     2221 ttcgccattt aaagtggtac gcgagctggg tttagaacgt cgtgagacag ttcggtccct
     2281 atctgccgtg ggcgctggag aactgagggg ggctgctcct agtacgagag gaccggagtg
     2341 gacgcatcac tggtgttcgg gttgtcatgc caatggcact gcccggtagc taaatgcgga
     2401 agagataagt gctgaaagca tctaagcacg aaacttgccc cgagatgagt tctccctgac
     2461 cctttaaggg tcctgaagga acgttgaaga cgacgacgtt gataggccgg gtgtgtaagc
     2521 gcagcgatgc gttgagctaa ccggtactaa tgaaccgtga gg
//
LOCUS       DBKEKI010000265         2473 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.268, whole genome shotgun sequence.
ACCESSION   DBKEKI010000265 DBKEKI010000000
VERSION     DBKEKI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.268"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..496
                     /locus_tag="ACUQPX_004305"
     CDS             <1..496
                     /locus_tag="ACUQPX_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HHV9058747.1"
                     /translation="ALTEIFTQAKARQMIICADMIKPRLNETLDDICEALSYVDYLFP
                     NFAEAKLLTGKETLDEIADCFLACGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAI
                     DTIGAGDNFASGFIAALLEGKNLRECARFANATAAISVLSVGATTGVKNRKLVEQLLE
                     EYEG"
     gene            506..1486
                     /gene="ydjG"
                     /locus_tag="ACUQPX_004306"
     CDS             506..1486
                     /gene="ydjG"
                     /locus_tag="ACUQPX_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019170 - methylglyoxal reductase (NADH)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HHV9058748.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKLIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            1623..2381
                     /locus_tag="ACUQPX_004307"
     CDS             1623..2381
                     /locus_tag="ACUQPX_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HHV9058749.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
BASE COUNT          725 a          486 c          627 g          635 t
ORIGIN      
        1 agcgctaaca gaaattttta cgcaagcgaa agcccggcag atgatcattt gtgccgatat
       61 gatcaaaccg cggttgaatg aaacgctgga tgatatttgc gaagcattga gttatgtcga
      121 ttatctgttt ccaaattttg ccgaggcaaa attactcaca gggaaagaga cactggatga
      181 aattgctgac tgctttcttg cgtgcggcgt aaaaacggtg gtgattaaaa cgggtaaaga
      241 cggctgcttt atcaagcgtg gtgacatgac gatgaaggtg ccggcggtcg caggaataac
      301 cgccatcgac accattggcg cgggcgataa ctttgcttca ggttttattg cggcactgtt
      361 agaaggcaaa aatctgcgtg aatgcgcacg ctttgccaat gcaacggcgg ctatctcggt
      421 tctaagcgtc ggtgccacca ccggcgtaaa aaacagaaag ctggtcgaac aattgctgga
      481 agaatacgaa ggataatgaa ggcaaatgaa aaagatacct ttaggcacaa cggatataac
      541 gctttcgcga atggggttgg ggacatgggc cattggcggc ggtcctgcat ggaatggcga
      601 tctcgatcgg caaatatgta ttgatacgat tcttgaagcc catcgttgtg gcattaatct
      661 gattgatact gcgccaggat ataactttgg caatagtgaa gttatcgtcg gtcaggcgtt
      721 aaaaaaactg ccccgtgaac aggttgtagt agaaaccaaa tgcggcattg tctgggaacg
      781 aaaaggaagt ttattcaaca aagttggcga tcggcagttg tataaaaacc tttccccgga
      841 atctatccgc gaagaggtag aagccagctt gcaacgtctg ggtattgatt acatcgatat
      901 ctatatgacg cactggcagt cggtgccgcc attttttacg ccgatagctg aaactgtcgc
      961 agtgcttaat gagttaaagg ctgaagggaa aattcgcgct ataggcgctg ctaacgtcga
     1021 tgctgaccat atccgcgagt atctgcaata tggtgaactg gatattattc aggcgaaata
     1081 cagtattctc gaccgggcaa tggaaaacga actgctgccg ctatgtcgtg ataatggcat
     1141 tgtggttcag gtttattccc cgctagagca gggattgttg accggcacca tcactcgtga
     1201 ttacgttccg ggcggcgctc gggcaaataa agtctggttc cagcgtgaaa acatgctgaa
     1261 attgattgat atgcttgaac agtggcagcc actttgtgct cgttatcagt gcacaattcc
     1321 cactctggca ctggcgtgga tattaaaaca gagtgattta atctccattc ttagtggggc
     1381 tactgcaccg gaacaggtac gcgaaaatgt cgcggcactg aatatcaact tatcggatgc
     1441 agacgcaaca ttgatgaggg aaatggcaga ggccctggag cgttaaatac tcaacacgat
     1501 tcacctctgt ttctgtgaaa actgtgatga aaatctgtga ttcaaatagg ttattttgaa
     1561 agtaaacatc ggttatgcga taatcgcgct aatgtgatgt aaaaagatac aggggtgtaa
     1621 tcgtggcggc aaaagacagg attcaggcaa ttaagcaaat ggttgccaac gataaaaagg
     1681 tgacagtctc aaatttgagt gggatttttc aggtaaccga agaaaccatt cgccgcgatc
     1741 ttgagaagct ggaagatgaa ggctttttga ccagaaccta tggtggtgct gttttaaata
     1801 cagcgatgtt gacggagaat atccattttt ataagcgcgc ttcatcgttt tatgaagaga
     1861 agcagcttat tgcacgcaag gcactaccct ttatcgacaa taaaaccacc atggcagccg
     1921 attccagtag taccgtaatg gaattgctca aattgttaca ggaccgtagt gacctgacgt
     1981 tgctaaccaa ctcggcggaa gcgattcatg ttctggctca gtcagaaatt aaagtcgttt
     2041 caacgggtgg ggaactaaac aaaaatacac tttccctgca aggaagaatt actaaagaga
     2101 tcatcagccg ctaccatgtc gatatcatgg taatgagttg taaaggtctt gatattaaca
     2161 gcggcgcgct ggactctaat gaagcggaag ctgaaatcaa aaagacaatg atccgtcagg
     2221 cgacagaagt tgcgttattg gttgatcact ctaagtttga tcgtaaagct tttgtccagt
     2281 tagctgactt tagtcatatt aattatataa taactgataa atcaccgggt gcagagtgga
     2341 tagcattttg caaagacaat aatatccagc tggtgtggta atttaagcac acgttgtcaa
     2401 tattatttgt tcctgcgcct ggaaataaaa tgatgataaa gatgcggcaa gtgaatttgc
     2461 cgcaattgct acc
//
LOCUS       DBKEKI010000266         2451 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.269, whole genome shotgun sequence.
ACCESSION   DBKEKI010000266 DBKEKI010000000
VERSION     DBKEKI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.269"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..332
                     /gene="ynaI"
                     /locus_tag="ACUQPX_004308"
     CDS             <1..332
                     /gene="ynaI"
                     /locus_tag="ACUQPX_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HHV9058750.1"
                     /translation="RRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
     gene            complement(383..1996)
                     /gene="mppA"
                     /locus_tag="ACUQPX_004309"
     CDS             complement(383..1996)
                     /gene="mppA"
                     /locus_tag="ACUQPX_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HHV9058751.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2161..2235)
                     /locus_tag="ACUQPX_004310"
                     /pseudo
     CDS             complement(2161..2235)
                     /locus_tag="ACUQPX_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004170112.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(2333..>2451)
                     /locus_tag="ACUQPX_004311"
     CDS             complement(2333..>2451)
                     /locus_tag="ACUQPX_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058752.1"
                     /translation="LDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT          657 a          582 c          539 g          673 t
ORIGIN      
        1 accgccgcat taccacgacc attggtttac gttatgagga tgcggcaaaa gtgggcgtta
       61 ttgtcgaagc cgtacgtgag atgctgaaaa atcacccggc catcgaccag cgacaaacct
      121 tactggttta tttcaaccag tttgctgact cgtcattgaa tattatggtt tattgcttta
      181 ccaaaaccac ggtatgggct gagtggcttg ctgcacagca agacgtttat ttgaagatta
      241 tcgatattgt acagtcacac ggcgcggact ttgccttccc cagccagacg ctgtatatgg
      301 ataacattac accgccggaa cagggtcgct aaatcagaca tcactggcgt tatccacaaa
      361 ggtaacgcca gttcccatca catcaatgct tcacaatata catagtccga ctgtacgcta
      421 catcttcagg gttattaatg ggataacctt tcagccacgg tttgattaat cgtccattgg
      481 tatattgata aattggtgca atcggtgctt gctccatgag gattttttct gccgcgttgt
      541 aatcggcatt acgcgcttta acggtatttt ccgtcgatgc ctgggccaga actttgtcgt
      601 atgccggatt gttaaagcgt gaaatatttc ctgaatgcat tgacgttaat aatgtcagga
      661 aagtggaggg ttcattataa tcacccaccc atgaggcgcg gataacatca aaattgccgg
      721 tgttacggct atcgatatag gttttccatt cctgattttg caatttaaca tctacgccaa
      781 ggttcttttt ccacatcgat gctacagcaa tcgcaatttt ttgatggttt tctgaagtgt
      841 tatacaaaag cgtcagcttc agcggttttt gcggaccata accagctgcg ctcaacaaag
      901 tttttgcctg cgcattcagt tcttcctgac tcatttgttc aaacggcgaa ggttccggcg
      961 taaatccggc ggtaacatct ggtataaaat gccacgctgg cttttcgccc gtccccaaca
     1021 ctttttcggt catcaggcgg cgatctatcg tcatacttaa tgccaggcga acgcgctgat
     1081 ctgccgtcgg ccctttttgc gtgttaaagg cataataata ggtcccgagc tgcggtggcg
     1141 tataaacctg ccccggaata tccttcaaca gcttctgata catatttttc gggaaggatt
     1201 cggtgatatc aatatccccc gcgaggtaac gcttagtggc tgcggattcc tgattaattg
     1261 gcaggaaggt cactttttgc agcaccgttt tggcgttatc ccaataatgg gtattcggta
     1321 ccacgaccag tttttcattg actacgcgct ctttaagaac ataagcgcca ttgccgatca
     1381 gatttccggg tttcgtccac tctttaccgc tttctacgtt ggctttttgc accgggaaga
     1441 aggcaaagtt agcggttaaa ttcacaaacc acggcaacgg tttatcaagc tgaattttca
     1501 aagtatgggc atcaactgcg gtgacgccaa gctgatcagg cgtagcttta ccatcaataa
     1561 tcgcctgtgc gttgttgatt cccgccagcg cggcaaacca tgcaaatggc gacaatgttt
     1621 ttgggtccac cagacgttgc cagctgtaga caaaatcttg cgccgttacc ggtgtgccat
     1681 ccgcccattt tgcgttatcg cgcagggtaa aagtccagat acggttgtca ttacttttcc
     1741 attgagtcgc gacgccgggg acaatctccc ctttttcgtt ctgattcacc agaccttcaa
     1801 acagatcgcg aatgacctga atctctggca ggcccacggc tttagcggga tccagcgacg
     1861 caggctcatc tttaatgtgg cgcaccagct cctgcttctc tgccagtact gtgccgctcg
     1921 gaacttctgc agcatacgaa agagaaatgc tgctgaccaa cagcgcacaa cacgtaactg
     1981 aaacagagtg cttcataagt ttccctctaa ccgacattaa aattgatgcg taattatttg
     2041 tttcgcctta aagaaatgca aacaacttcc atcaaaaaag tgacaaagga agcatcaaat
     2101 gttttaagta tcaatatatc aaatcagatt tagtgtaaat aatagacgcc aaaatgcggc
     2161 aaagcacata gaacaccgaa cactacgttc gggcttcgca cgttcaaacc agttttcggt
     2221 atcataatca gcatctgtcg ccattgtacg aagaataaaa aacaaccaaa acgttgaaag
     2281 gaactgagaa tacgttaaga gaagaattaa attaatgtat cagcaagtag tgctaaacag
     2341 cacctttata cttcagccta tctataaata atgagaatgc cgagccagca tgtcgacggt
     2401 gtggatagta caggtgataa ccgggtaaat ctggagtgaa tttatccaaa a
//
LOCUS       DBKEKI010000267         2450 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.270, whole genome shotgun sequence.
ACCESSION   DBKEKI010000267 DBKEKI010000000
VERSION     DBKEKI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.270"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            32..550
                     /locus_tag="ACUQPX_004312"
     CDS             32..550
                     /locus_tag="ACUQPX_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HHV9058753.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     SFQGKEWVHLKE"
     gene            557..790
                     /locus_tag="ACUQPX_004313"
     CDS             557..790
                     /locus_tag="ACUQPX_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HHV9058754.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
     gene            complement(1092..1484)
                     /locus_tag="ACUQPX_004314"
     CDS             complement(1092..1484)
                     /locus_tag="ACUQPX_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058755.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQQLQ
                     IQEVCNSTLWETAKQQLLQESDGRYFAINSKHLDFPGSGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFSHNLT"
     gene            complement(1801..1959)
                     /locus_tag="ACUQPX_004315"
     CDS             complement(1801..1959)
                     /locus_tag="ACUQPX_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HHV9058756.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAASFGNWIRWIEI
                     LRVLEVVA"
BASE COUNT          629 a          508 c          700 g          613 t
ORIGIN      
        1 gggaagaata tcaggctgaa aggagcattc tatgagcaat cttttacaga cgggcgcgga
       61 gttcgagaaa aagctgaaag agagagccga atctacagag aaaatgctga acgacgagtt
      121 caggaagctc gaagaatctg taagcaaggc tgtgacatca aacgagacga aaatcaaaga
      181 cgctatagcc ctgttcacag cttcgacaga ggaatcactg aaaaaacacc gggaaggggt
      241 gaaagaggcg atgatgcagc acaggaagga cgtgttaaag ctggcaggga atacgggcat
      301 gatgttactg gggatggtca ttttcctgtt taccgtgagt ggcgggacgc tctggtacct
      361 tggcgggatg atacaggcga acctggaaga aatcaggaag cagaacgaga gtctggagaa
      421 gctgaacgcg aagacatggg gcgtggagtt tgtgcaggac gggaacagga aatttctggt
      481 ccttccttac gggaagtcag cagaggtgat ttcctttcag gggaaggaat gggtacatct
      541 gaaggagtga cacagagtga cagagctgga aacacatttg ctgaacgcct tagagcagct
      601 gcaacaggac tatatgcagc ggctgagcga atgggagagc gccttcgtgg aattgcagaa
      661 aatgttttcg cttacgcaac gggacaacgc gatgctgaac gagcgggtca tgcagttgag
      721 tcagcaggtg cagcacttga gcgagcagac agaacgcttg agccagttat acagcgagaa
      781 ctggagataa gagaggaacg gctgatacag gagcgcgaac atgcgttatc cctggaacgg
      841 gagcgtcagc cggaaataca ggaacgcacg ctggatggtc cttcgctggg atggtgaaac
      901 catgaaaaat ggcagcttca gtggattagg aggggttaat gtggcctgta ccatctggtt
      961 gcataggtat tcatgcggtt aaaatttatc aggcgcgatc gcggcagttt ttcgggtggt
     1021 ttgttgccat ttttatctgt ctgctgccgt gatcgcacca aacgcgtttc agtggtgcgg
     1081 gcaataagat attacgttaa attgtgagag aatggtcccc atatactgac tttatcatac
     1141 ggatagggtt ggtagtgggt cgattgggtt tcggcattgt ttccgttaat gccaattgca
     1201 tttttgtatt tgatgcaaca gaaggcgtga gattctcctg agcctggaaa gtctaagtgt
     1261 ttagaattga tggcaaaata tcttccgtcc gattcttgaa gtagttgttg tttggctgtc
     1321 tcccaaaggg tgctgttaca aacttcttga atttgtagtt gttgcaggac tcttattact
     1381 gcgttgtcat gttccaattc tttaggggag tgtattattc tctcttttaa taagctgttt
     1441 accacaggat ttagttgttg tctaaagcac gcagataatg ccattgctac acaattttgt
     1501 tcccgaggaa gcatgtttgg cggggtatgc cgtgtttgat gtgatatgtc tattctcata
     1561 gaaggtctct ggcttatagc atcaacaaat acggctataa aacaaaattt tcatagagtg
     1621 attcaagatc ttcttgagat ctttttttgc ggtgcgtaat ctgctgcctg caaacaaaaa
     1681 aaccaccgtt atcaacggtg gtttgtttgc cggaatctag agctaccaac tctttcttcc
     1741 gaaggtaact ggcttggtgg agcacagata ccaaatgctg tccttctagt gtagccgcag
     1801 ttaggccacc acttcaagaa ctcttaatat ctcaatccac cttatccagt taccaaagga
     1861 tgctgccagt ggcgttaagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1921 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1981 gacctacacc gaactgagat accaacagcg tgagctatga gaaagcgcca cgcttcccga
     2041 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2101 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2161 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2221 cggcgcagcc tttcgttgcc ctttgccttt ttgctcacat gttctttccg gccttatccc
     2281 ctgattctgt ggataaccgt attaccgtct ttgagtgagc tgataccgct cgccgcagcc
     2341 gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga agagcgccgg aagctgcatt
     2401 ttctccttac gcatctgtgc ggcatttcac atcggctatg gtgcactttt
//
LOCUS       DBKEKI010000268         2448 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.271, whole genome shotgun sequence.
ACCESSION   DBKEKI010000268 DBKEKI010000000
VERSION     DBKEKI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.271"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1374)
                     /gene="caiC"
                     /locus_tag="ACUQPX_004316"
     CDS             complement(<1..1374)
                     /gene="caiC"
                     /locus_tag="ACUQPX_004316"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HHV9058757.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDHPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSI"
     gene            complement(1448..>2448)
                     /gene="caiB"
                     /locus_tag="ACUQPX_004317"
     CDS             complement(1448..>2448)
                     /gene="caiB"
                     /locus_tag="ACUQPX_004317"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HHV9058758.1"
                     /translation="FKDEGREAFLKLMETTDIFIEASKGPAFARRSITDEVLWQHNPK
                     LVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQPMPAFPYTADYFSGLT
                     ATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNGGEMCPRMSKGKDPYY
                     AGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPEGTQLIHRIECPYGPL
                     VEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQYVARESITQWQTMDGR
                     TCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDIQELVSKGLAKVED"
BASE COUNT          553 a          644 c          629 g          622 t
ORIGIN      
        1 aatcgaatct ttaataccca caaccacgat gtcctgaatt ttcggatggg tggcgatgat
       61 attttccagc tccacgcagg agacattctc gccaccgcgt ttgatcatat tgcagcggcg
      121 atcgacgaaa taaaaaaagc cttcttcgtc gcggtatccg gtatcgccgg tatgtagcca
      181 gccgtcggct tccagcactt tcgcagtggc tttcgggttg agaaaatact ctttgaagat
      241 ggttttccct ggtacgcctt taatacagat ttcaccgatc tcacctgccg ggagcgggcg
      301 attgtgatcg tcgcggatct ccgcttcgta gcaaaacccc gcccgaccaa tcgacggcca
      361 gcgtcgttta tcgctagggt gatcgccgat aatgcccaca atggtttccg tcatcccata
      421 agacgtcagc aagcgaacgc cgaagcgttc acaaaatgcg tctttttcct gctccgacaa
      481 gttgagataa aacatcactt cccgcaggcg gtgttgccga tcgttcgctg aaggcggctg
      541 caccatcaac gtacggatca tcatcggaat acattcggta atggtggcgc ggtacttctg
      601 cgcctgtccc cagaaggcgc gggcgctgta tttctcgacc agcacaaagg tggccccggc
      661 agaaaacgcc gccatcgccg cagtacactg gcaatcgata tgaaacgcag gcattaccgt
      721 catgtagacg tcatcgtcac gcagtgcaca ctgccaggcg gagtaatatc cagcgaagcg
      781 caggttgtaa tgggtaatca ccacaccttt cggtcgggag gtggtgccgg aggtgaagag
      841 aatttccgcc gtatcgtcag tcgatagcgg cggtgcatag cacaaggtgg caggttgttg
      901 atttttcagt tgagtaaacg aactcactcc atcatcagcg ggaagtgcca catctgtcag
      961 gcaaatgtgc cgcaattgag tggcatcttc ctgctgaatc tgttgataca taggataaaa
     1021 ttgcgcactg gtcaccagca ggcacgcctg gctattttgc aagatccact cgctttcctc
     1081 gcgtaacagg cgggcgttaa tcggcaccat aatcgcgcca atttttgcca gcccgaacca
     1141 gcaaaagata aattccgggc agttgtcgag atgtagtgca accttgttgc ctttgcgaat
     1201 ccccagcgta taaaacaggt ttgccgtgcg gttaatctcc tgatttaact caagataact
     1261 ataccggtta acgactccgc cgctggattc acaaatcagc gccgttttat gaccgtaaac
     1321 gtccgccaga tcgtcccaca tttgacgtag atgttgtccg ccaatgatat ccattgcacc
     1381 tctatccatt tttgttcgtt tgttattggg cgggcgctag tcaggcaagc cgactgacgc
     1441 cacgcgttta gtcctcaact ttggccagac ctttgctgac caactcctga atgtcgtttt
     1501 cgctgtagcc gatatttttc aaaatggcag ccgtgtccat gccatgtgag ggcattccgc
     1561 gccagatttg tccggggtta tttttgaatt tcggcatgat gttcggccct ttgcaggtgc
     1621 gaccatccat cgtttgccac tgagtgattg attcacgggc aacatactgt ggattgcttt
     1681 ccagttccgg tacggtcagt actttggcac aggcgatatt cagttcggca aagcgttctt
     1741 taacttctgc gatggtatgt gccgccagcc aggcatcgag tttttcttct accagtgggc
     1801 cgtaagggca ttcgatacgg tggataagct gagtgccttc cgggatttcc ggcgtaccga
     1861 gtagatgtgc gaggccaata tctttaaagc actcttcaat ttgggtaatg cccaccagtt
     1921 ccatcacgat gtagccgtcg gcacatttat acagaccgca accggcgtag tagggatctt
     1981 tacctttgct catgcgcggg cacatttcgc cgccgttgaa gtaatccatc atgaagtact
     2041 ggcccatacg cagcatcact tcatacatgg cgatgtcgat actttcgcct ttaccggttt
     2101 cacgcacttt atgcagtgct gccagcgccg ccgtggtggc ggtcaggcca gaaaagtaat
     2161 cggcggtata cgggaaggca ggcattggct ggtcaacatc accgttctga atcaggtaac
     2221 cactgaaggc ctgggcgatg gtgttatagg ccggaagatt ggtgtactcc tcggtgccgt
     2281 actgaccaaa accggacagg tgagcgataa ccagtttcgg gttgtgctgc cacagtactt
     2341 catcggtaat gctacgacgg gcaaaggccg gacctttact ggcttcgatg aagatatcgg
     2401 tggtttccat taatttcaga aacgcttcgc ggccttcatc tttgaaaa
//
LOCUS       DBKEKI010000269         2397 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.272, whole genome shotgun sequence.
ACCESSION   DBKEKI010000269 DBKEKI010000000
VERSION     DBKEKI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.272"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..445
                     /locus_tag="ACUQPX_004318"
     CDS             <1..445
                     /locus_tag="ACUQPX_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HHV9058759.1"
                     /translation="YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERD
                     KHLRSADFLNTTKYPQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDD
                     PWGGKRAGFEAEGKIKLKDFNIKTDLGPASQEVDLIISVEGIQQK"
     gene            complement(487..1470)
                     /locus_tag="ACUQPX_004319"
     CDS             complement(487..1470)
                     /locus_tag="ACUQPX_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HHV9058760.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPDALFIDMRNHYEYEVGHFENALEIPADTFREQLPK
                     AVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGFNKVWHIEGGIIEYARKAREQGLP
                     VRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDSHTNCKNDGCHLLFIQCPVCAEKY
                     KGCCSEICCEESALPPEEQRRRRAGRENGNKIFNKSRGRLNTTLGIPDPTE"
     gene            1695..>2397
                     /gene="lpxL"
                     /locus_tag="ACUQPX_004320"
     CDS             1695..>2397
                     /gene="lpxL"
                     /locus_tag="ACUQPX_004320"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HHV9058761.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RITRWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGA"
BASE COUNT          622 a          589 c          588 g          598 t
ORIGIN      
        1 ctatagctgg ttatatggca cctttaaaga tttcgacggt acttttacct ttgatgaaaa
       61 aaatccggct gccgataaag tgaatgtgac aattaacacc accagcgtcg atactaacca
      121 cgccgaacgc gataaacatc ttcgcagtgc ggattttctc aataccacaa aatatccaca
      181 ggcaacattc acctccacca gcgtgaagaa agacggtgac gaactggata ttaccggcga
      241 tctgacgctg aatggcgtaa ccaaacccgt cacgctggaa gcgaaattaa ttggtcaggg
      301 cgacgaccca tggggcggta aacgtgcagg ctttgaggcc gaaggcaaaa ttaagctcaa
      361 agacttcaat atcaagacag atttaggtcc agcttctcag gaagtggatc tgattatttc
      421 agtggaaggc atacagcaga agtaagagtg aaatgttgcc ggatggcacg catccggcaa
      481 tgatatttat tctgttggat caggaatacc cagtgttgta ttcagacgtc cacgagactt
      541 attaaagatc ttattgccat tttcacgtcc tgcccgacgg cgtcgctgtt cttctggcgg
      601 taacgcgctt tcttcgcagc aaatctcact acaacaacct ttgtattttt ccgcgcatac
      661 tggacactga ataaacagca ggtggcagcc atcattttta cagttggtat ggctgtcgca
      721 cggcgcgccg cactggtggc aatgcgcgat aatctcatcc gatatacgtt cgcccatccg
      781 ctcgtcaaaa acaaaatttt tgccaataaa acgcaccggc aagccctgct cgcgcgcctt
      841 acgggcgtat tcaataattc cgccctcgat atgccagact ttgttgaatc cgttatgttt
      901 catccaggca ctggcctttt cacaacgaat gccgccggtg cagtacatga cgattttttt
      961 atctttatgt gcctgcatca tctcgactgc ttttggcagc tgctcacgga aggtatctgc
     1021 cggaatttcg agcgcgtttt caaagtgccc cacttcatac tcatagtggt tacgcatgtc
     1081 gataaatagt gcatcgggat cgtcaatacc gtcggcaaca atgcgatcgc gtaccttcat
     1141 gcgcagtacc cagaaggatt tcccgtcatc atccaacgcg atattcaggc gtaaaccctc
     1201 taaagccggg tcgaaggcat agagctgcgc gcgaaatgtt tcaacattgc tcgcaggtac
     1261 gctgatttgc gcgttaatgc cctcatgcgc cagatacact cgcccaaaaa cattcagcgc
     1321 ggtaaacagc tgatataaag cgtcacgggt cgccttagga tcggcgatgt ggaaatactt
     1381 gtaaaacgaa atggtggttc gcggttcgct ctcagccaac attttggctt ttaacgcgtc
     1441 gttggaaatg cggttgtgta acactggcat ggtgtacggt tcctgcgaga tgggaaagta
     1501 aaaatccgcg gcatgatata gcaattatcg ataattaaca tccacacatt ttacgctaca
     1561 tttgcgcatt aaaaattatt tgttatttac aagcgcggca atttcgccca gtcttcagcc
     1621 acaaattttg gttgcgggcg aaaaaatgcg acaatacata caattgcccg tataggttga
     1681 aaaacaggat tgatatgacg aatctaccca agttctccac cgcactgctt catccgcgtt
     1741 attggttaac ctggttgggt attggcgtac tttggttggt cgtgcaattg ccctacccga
     1801 ttatctaccg cctcggttgt ggattaggaa aactggcgtt acgttttatg aaacgacgcg
     1861 caaaaattgt gcatcgcaac ctggaactgt gcttcccgga aatgagcgaa caagaacgcc
     1921 gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg cctgatggaa accggtatgg
     1981 cgtggttctg gccggaccgc cgaatcaccc gctggacgga agtgatcggc atggaacaca
     2041 ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt tggcatccat tttctgacac
     2101 tggagctggg cgcgcggcag tttggtatgc aggaaccggg gattggcgtt taccgcccga
     2161 acgataatcc actgattgac tggctacaaa cctggggccg tttgcgctca aataaatcga
     2221 tgctcgaccg caaagattta aaaggcatga ttaaagccct gaaaaaaggc gaagtggtct
     2281 ggtacgcacc ggatcatgat tacggcccgc gctcaagcgt tttcgtcccg ttatttgccg
     2341 ttgagcaggc tgcgaccacg accggaacct ggatgctggc acggatgtcc ggcgcat
//
LOCUS       DBKEKI010000270         2387 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.273, whole genome shotgun sequence.
ACCESSION   DBKEKI010000270 DBKEKI010000000
VERSION     DBKEKI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.273"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..241
                     /locus_tag="ACUQPX_004321"
     CDS             <1..241
                     /locus_tag="ACUQPX_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HHV9058762.1"
                     /translation="GCHHTAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEG
                     WTTMTITTPTGEGVTSARTFVRLRRCVLVDAFRIV"
     gene            252..1088
                     /gene="eutJ"
                     /locus_tag="ACUQPX_004322"
     CDS             252..1088
                     /gene="eutJ"
                     /locus_tag="ACUQPX_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HHV9058763.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVM
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            1078..2265
                     /gene="eutG"
                     /locus_tag="ACUQPX_004323"
     CDS             1078..2265
                     /gene="eutG"
                     /locus_tag="ACUQPX_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HHV9058764.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQLRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSERKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDSD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
BASE COUNT          481 a          631 c          769 g          506 t
ORIGIN      
        1 cgggtgccac cacacggcgg caatgcactc gcgcaatatc gagaacatga accagatggc
       61 gaatgccatt gataccagca ttttcgttaa gaacggaccg tgcattgccg ggctgggact
      121 gggcggggaa ggctggacca ccatgaccat caccacgcca accggtgaag gggtaaccag
      181 cgcgcgtacg tttgtccgtc tgcgtcgctg tgtattagtc gatgcgtttc gcattgttta
      241 aggagtaacc gatggcgcac gacgaacaat ggctcacccc acgtctgcaa acagcggcaa
      301 cgctgtgtaa ccagacgcct gccgcgacgg aatccccgct gtggctgggg gtggatctgg
      361 gcacctgcga tgtggtgtcg atggttgtcg accgcgacgg tcagccggtg gcggtatgcc
      421 tcgactgggc cgatgtggtc cgcgacggca tcgtctggga tttcttcggc gcggtcacca
      481 ttgttcgtcg ccatctcgac acgctcgagc agcaattcgg tcgtcgtttt agccatgcgg
      541 cgacttcatt tcctccaggt acggacccgc gtatttccat caacgtgctg gaatctgccg
      601 ggctggaagt gagccatgtg ctggatgagc caaccgcagt cgcggatcta ctgcaactgg
      661 ataacgccgg tgtagtggat atcggcggtg gcactaccgg cattgccatc gtgaaaaagg
      721 gcaaagtgat gtactcggcg gatgaagcca ccggcggaca tcacatctct ctgactctcg
      781 ccggaaatcg ccgtatttcg ctggaagagg cggagcagta caagcgcggt cacggtgaag
      841 agatttggcc tgcggtgaaa ccggtgtacg aaaaaatggc ggacattgtc gcccggcata
      901 ttgaagggca gggcattact gatttatggc tggcgggcgg ctcctgtatg caaccgggcg
      961 tggcagaact gtttcgcaaa caattcccgg cgttacaggt gcatttaccg cagcacagtt
     1021 tgtttatgac cccgctggcg atcgccagta gtgggcgaga gaaagcggag gggctatatg
     1081 caaaatgaat tgcagaccgc gctctttcag gcgttcgata ccctgaatct gcaacgggta
     1141 aaaacattta gcgttccacc ggtgacgctt tgcggtccgg gctcggtgag cagttgcgga
     1201 cagcaagcgc aaacgcgtgg gctgaaacat ctgttcgtga tggcagacag ctttttgcat
     1261 caggcaggga tgaccgccgg gctgacgcgt agcctggccg ttaaaggtat cgccatgacg
     1321 ctctggccat gtccggtggg cgaaccgtgc attaccgacg tgtgtgcagc cgtggcgcag
     1381 ttgcgtgagt caggttgtga cggggtgatc gcatttggcg gcggctcggt gctggatgcg
     1441 gcgaaagccg tggcgttgct ggtgacgaac ccggatagca cgctggcaga gatgtcagaa
     1501 accagcgttc tgcaactgcg cttgccgctg attgccattc caactaccgc cggaaccggc
     1561 tctgaaacca ccaatgtaac ggtgattatc gacgcggtga gcgagcgcaa gcaggtgtta
     1621 gcccatgcct cgctgatgcc ggatgtggcg atcctcgacg ccgcattgac cgaaggtgtg
     1681 ccgtcgcatg tcacggcgat gaccggcatt gatgcgttaa cccatgccat tgaagcatac
     1741 agcgccctga acgctacacc gtttaccgac agtctggcga ttggtgccat tgcgatgatt
     1801 ggcaaatcgc tgccgaaagc ggtgggctac ggtcacgacc ttgccgcgcg cgagagcatg
     1861 ttgctggctt catgtatggc gggaatggcg ttttccagtg cgggtcttgg gttgtgccac
     1921 gcgatggcgc atcagccggg cgcggcgctg catattccac acggtctcgc gaacgctatg
     1981 ttgctgccaa cggtgatgga gtttaaccgg atggtttgtc gtgatcggtt tagtcagatt
     2041 ggtcgggcgc tgcgaactaa aaaatccgac gatagtgacg ctattaacgc ggtaagtgag
     2101 ctgattgctg aagttgggat tggtaaacga ctgggcgatg ttggtgcgac atctgcgcat
     2161 tacggcgcat gggcgcaggc cgcgctggaa gatatttgtc tgcgcagtaa cccgcgtacc
     2221 gccagcctgg agcagattgt cggcctgtac gcagcggcgc aataaatgcc ggatgcgacg
     2281 cttgccgcgt cttatccagc ctacgggatt gcacatgtag ggcggataag gcgttcacgc
     2341 cgcatccgcc agtggcgcgg tgcaattgcc ggatgcgatg cttgccg
//
LOCUS       DBKEKI010000271         2362 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.275, whole genome shotgun sequence.
ACCESSION   DBKEKI010000271 DBKEKI010000000
VERSION     DBKEKI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.275"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..457
                     /gene="istB"
                     /locus_tag="ACUQPX_004324"
     CDS             <1..457
                     /gene="istB"
                     /locus_tag="ACUQPX_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HHV9058765.1"
                     /translation="GPSGVGKTHLAIAMGYEAVRAGIKARFTTAADLLLQLSTAQRQG
                     RYKTTLQRGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQW
                     DQTFAGDAALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            619..1827
                     /locus_tag="ACUQPX_004325"
     CDS             619..1827
                     /locus_tag="ACUQPX_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HHV9058766.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
                     GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIR
                     EQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVS
                     DAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKR
                     LTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWILATNLPVEIR
                     TPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWL
                     AGVHAQKQGWDKLFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDSLVAATLLTQNL
                     FTHGYVLGKL"
     gene            1827..2362
                     /locus_tag="ACUQPX_004326"
                     /pseudo
     CDS             1827..2362
                     /locus_tag="ACUQPX_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          691 a          541 c          515 g          615 t
ORIGIN      
        1 ggggccatca ggtgtgggga aaacccatct ggcaatagcg atgggctatg aagcagtccg
       61 tgcaggtatc aaagctcgct tcacaacagc agcagatctg ttacttcagt tatctacggc
      121 acaacgtcag ggccgttata aaacgacgct tcagcgtgga gtaatggccc cccgcctgct
      181 catcattgat gaaataggct atctgccgtt cagtcaggaa gaagcaaagc tgttcttcca
      241 ggtcatcgct aaacgttacg aaaagagcgc aatgatcctg acatccaatc tgccgttcgg
      301 gcagtgggat caaacgttcg ccggtgatgc agcactgacc tcagcgatgc tggaccgtat
      361 cttacaccac tcacatgtcg ttcaaatcaa aggagaaagc tatcgactca gacagaaacg
      421 aaaggccggg gttatagcag aagctaatcc tgagtaaaac ggtggatcaa tattgggccg
      481 ttggtggaga tataagtgga tcacttttca tccgtcgttg acactaatga tttttatcaa
      541 aatcattaag ttaaggtaga tacacatctt gtcatatgat caaatggttt cgccaaaaat
      601 caataatcag acaacaaaat gtgcgaactc gatattttac acgactctct ttaccaattc
      661 tgccccgaat tacacttaaa acgactcaac agcttaacgt tggcttgcca cgccttactt
      721 gactgtaaaa ctctcactct taccgaactt ggccgtaacc tgccaaccaa agcgagaaca
      781 aaacataaca tcaaacgaat cgaccgattg ttaggtaatc gtcacctcca caaagagcga
      841 ctcgctgtat accgttggca tgctagcttt atctgttcgg gcaatacgat gcccattgta
      901 cttgttgact ggtctgatat ccgtgagcaa aaacggctta tggtattgcg agcttcagtc
      961 gcactacacg gtcgttctgt tactctttat gagaaagcgt tcccgctttc agagcaatgt
     1021 tcaaagaaag ctcatgacca atttctagcc gaccttgcga gcattctacc gagtaacacc
     1081 acaccgctca ttgtcagtga tgctggcttt aaagtgccat ggtataaatc cgttgagaag
     1141 ctgggttggt actggttaag tcgagtaaga ggaaaagtac aatatgcaga cctaggagcg
     1201 gaaaactgga aacctatcag caacttacat gatatgtcat ctagtcactc aaagacttta
     1261 ggctataaga ggctgactaa aagcaatcca atctcatgcc aaattctatt gtataaatct
     1321 cgctctaaag gccgaaaaaa tcagcgctcg acacggactc attgtcacca cccgtcacct
     1381 aaaatctact cagcgtcggc aaaggagcca tggattctag caactaactt acctgttgaa
     1441 attcgaacac ccaaacaact tgttaatatc tattcgaagc gaatgcagat tgaagaaacc
     1501 ttccgagact tgaaaagtcc tgcctacgga ctaggcctac gccatagccg aacgagcagc
     1561 tcagagcgtt ttgatatcat gctgctaatc gccctgatgc ttcaactaac atgttggctt
     1621 gcgggcgttc atgctcagaa acaaggttgg gacaagctct tccaggctaa cacagtcaga
     1681 aatcgaaacg tactctcaac agttcgctta ggcatggaag ttttgcggca ttctggctac
     1741 acaataacaa gggaagactc actcgtggct gcaaccctgc ttactcaaaa tctattcaca
     1801 catggttacg ttttggggaa attatgaggg gatctctcag cactgagcta agttctgtgg
     1861 cgaatatatc agcttcaacg gcgagcagtc atttatctaa attattagat tgccagctaa
     1921 tcacagtagt agctcaaggc aagcatcgtt attttcggct agcaggaaaa gatattgctg
     1981 aattgatgga aagtatgatg gggatctcct taaaccatgg cgtacatgcc aaagtttcca
     2041 cgccagtgca tttacgaaaa gcacgtactt gctatgatca tttagctggc gaagttgccg
     2101 ttaagatcta tgattccctt tgtcaacagc aatggatcac tgaaaatggt tcaatgatca
     2161 cattaagtgg tattcaatat tttcatgaaa tgggaattga cgttccttcc aaacattcac
     2221 gtaaaatctg ttgtgcgtgt ttagattgga gtgaacgccg tttccattta ggtgggtacg
     2281 ttggagccgc attattttcg ctttatgaat ctaaagggtg gttaactcga catcttggtt
     2341 accgtgaagt taccatcacg ga
//
LOCUS       DBKEKI010000272         2305 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.276, whole genome shotgun sequence.
ACCESSION   DBKEKI010000272 DBKEKI010000000
VERSION     DBKEKI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.276"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            17..970
                     /locus_tag="ACUQPX_004327"
                     /pseudo
     CDS             17..970
                     /locus_tag="ACUQPX_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1011..2006)
                     /gene="pgl"
                     /locus_tag="ACUQPX_004328"
     CDS             complement(1011..2006)
                     /gene="pgl"
                     /locus_tag="ACUQPX_004328"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HHV9058767.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          625 a          576 c          532 g          572 t
ORIGIN      
        1 attttccgga gtaataatga agcatgaatt atcaagtatg aaggcatttg tcatactggc
       61 agagtccagt tcatttaaca atgccgctaa attactcaat attacgcaac ctgcattaac
      121 gcgaagaata aaaaagatgg aggaagattt acatattcaa ctatttgagc gtacaactcg
      181 caaggttacg ttaacaaaag caggaaaaag gttgctcccg gaggcgcggg aattaataaa
      241 gaaattcgat gaaacgcttt ttaatattcg tgatatgaat gcttatcatc gtggtatggt
      301 gacattagcg tgtattccga ccgcagtatt ttatttttta ccgctggcaa tcggtaaatt
      361 taatgagctg tatcccaata ttaaagtgcg gattctggaa caaggcacga ataattgcat
      421 ggaatcggtg ctgtgtaacg aatcagactt tggcataaac atgaacaacg tcacaaattc
      481 atccattgat tttaccccac tggtcaacga gccattcgtg ctggcctgtc ggcgtgacca
      541 tccattggcc aaaaagtagc ttgtagaatg gcaggagtta gtgggttaca aaatgattgg
      601 cgtacgttcc tccagcggca accgattgtt gatagaacaa cagctagccg acaagccctg
      661 gaagctggac tggttttacg aagtgcgtca tctttcgacg tcattaggac tagtggaggc
      721 aggactgggg atttcggcgc ttcctggtct ggcaatgccc catgcgccct attcttcgat
      781 tattggtatc ccactggtgg aacccgttat acgtcggaca ttggggatta ttcgccgcaa
      841 agatgccgtg ctttctccgg cagctgaacg cttttttgcc ttgctgatta atttgtggac
      901 tgacgataaa gacaatttgt ggaccaatat cgtcgaacgc cagagacatg cgttacagga
      961 gataagttaa tacaccggcg caggagagac tgcgccggtt aaatcagcgg ttagtgtgcg
     1021 ttaaccacca cccacattgg tccctgcccg accgcatagc ggcctttttc atgcagtagc
     1081 ccctgctcgc caacaatttc gtataccgag atgtggtgag atttttgccc agcggcaatc
     1141 agatacttgc cgctgtaatc aacattgaag ccgcgcggct gggtttccgt tggctggaag
     1201 ccttctttac tcaacacgct gccatcttcc gaaacgctga aaacggtaat cagactggcg
     1261 gtacggtcgc aggcgtataa atggcgacca tccggggtga tatgaatatc agccgcccaa
     1321 cgggtgtcgg agaagttttc cggcatcata tccagcgtct ggacacattc gatattgccg
     1381 tgcggatctt tcagttccca gacatccact gagctgttta actcattgac gcaatacgca
     1441 tattgttcgt ttggatggaa taccatatga cgcgggccgg ccccttcaac ggtagtcact
     1501 tccgcagggt cctgcgccac cagatgacca tcatcgctga ccgtaaacag gcaaatgcga
     1561 tcctgcttta atgccggaac ccacagcgta cggttgtccg gtgagatatt ggcggaatgg
     1621 caaccgtcca gcccctcgac cacatcgacg acgcccactg gcaggccatc ttccagacgc
     1681 gttacgctca cgttacccgc attgtaagaa cctacaaaga caaactgccc ctggtgatcg
     1741 gtggaaatat gcgtcggact acccggcagc acagactctg cggcaaaggt cagtgcgcca
     1801 tcgtccgggg caatacgata cgccaggacg cgaaactcag ggcgaacacc aacatagaga
     1861 taacgtttgt ccgggctgac caccatcggc tgcacctgcc ccggcacatc gacaacctgt
     1921 gtcagcgtca gtgcgccttc atgattcagg ttccagacgt gaatttgctg gctctcaggg
     1981 ctggcgatat aaactgtttg cttcatgaac acacctcaaa aatgataaat cccttatctt
     2041 tcatacatat aacaacactt actggataaa aaacagacag caaaacacga taaaatacaa
     2101 tatcgcacat caattaaatc cataagttag cactccattt cgaatggcat attactaaaa
     2161 tattagtcac cgacacgaat ttgacaatgc cacagatatt ttcgcgacta ttcagggagt
     2221 atgactctcc ccgcttttgt ttacctgtat caacgaccgt aaacactatg ccataacaac
     2281 ttctggccaa cttcccattc gctca
//
LOCUS       DBKEKI010000273         2259 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.277, whole genome shotgun sequence.
ACCESSION   DBKEKI010000273 DBKEKI010000000
VERSION     DBKEKI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.277"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..519
                     /locus_tag="ACUQPX_004329"
     CDS             <1..519
                     /locus_tag="ACUQPX_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0043711 - pilus organization [Evidence
                     IEA];
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis chaperone"
                     /protein_id="HHV9058768.1"
                     /translation="WVDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENKE
                     SLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWKSLEFKRSD
                     HSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDY
                     GGSSGTETRPLQ"
     gene            548..>2259
                     /locus_tag="ACUQPX_004330"
     CDS             548..>2259
                     /locus_tag="ACUQPX_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HHV9058769.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVLGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIP"
BASE COUNT          678 a          530 c          494 g          557 t
ORIGIN      
        1 tgggtagata atatagatgg taaaagccgt gccccattta ttataacccc accgctattt
       61 cgtcttgagg ctggcgatga ctcatcactg cgaattatta aaacagctga taacctgcct
      121 gaaaataaag agtcgctgtt ctacattaat gttcgtgcca ttccagcaaa gaaaaaatca
      181 gatgatgtta atgctaacga gttgacgctg gtatttaaaa cacggatcaa aatgttttat
      241 cgccccgcac acctgaaggg acgggtaaac gatgcgtgga aatcactgga atttaaacgt
      301 agtgaccatt cactcaatat atataaccca actgaatatt acgtcgtatt tgccggactg
      361 gcagtcgata aaaccgatct cacaagcaaa attgaatata tcgcgcccgg agaacataaa
      421 cagttaccac ttccagcatc tggcggaaag aacgtgaaat gggctgcgat caacgattat
      481 ggcggcagtt ccgggacaga aactcgtcca ctgcaataaa aaatataaaa acacaggtca
      541 tcagggaatg cctcaacgac accaccaggg acataaacgc acaccgaaac agttggcgct
      601 cattatcaaa cgttgtttgc cgatggtgct cactggcagc ggcatgcttt gcactaccgc
      661 taacgccgaa gagtattatt tcgaccccat tatgctggaa actacaaaaa gtggtatgca
      721 aacaaccgat ctgtcacgtt tttcaaaaaa atacgcacaa ctaccaggaa cttatcaggt
      781 tgatatctgg ctgaataaaa agaaggtttc acagaaaaaa attacattta ccgccaatgc
      841 agagcaactt ctgcagccac agtttacggt agaacaacta cgtgagctgg gtattaaggt
      901 ggatgaaatc ccggcgctgg ctgaaaaaga tgacgatagc gtgatcaact cgcttgaaca
      961 aatcattccc ggtacagctg ctgaatttga tttcaatcat cagcgactta atttgagcat
     1021 tccccaaatt gcactgtacc gtgatgcaag aggttacgtc tccccttctc gttgggacga
     1081 tggtatacca acgctgttta ccaactactc gtttacaggt tctgataacc gttaccgcca
     1141 gggcaatcgt agccaacgac agtacctgaa tatgcaaaat ggtgctaatt ttggcccctg
     1201 gcgattacgt aactattcta cgtggacacg caacgatcag gcgtcaagct ggaacactat
     1261 cagtagttat ttacaacgtg atatcaaggc gttgaagtct cagttgcttc tgggagaaag
     1321 cgccaccagc ggcagtattt tttccagcta cacctttact ggcgtgcaac tcgcttccga
     1381 cgataatatg ttgccaaaca gccagcgcgg atttgcccca acggtactcg gtatcgcaaa
     1441 cagtagtgca atcgtgacta tcaggcaaaa tggttatgtg atctatcaaa gcaacgtgcc
     1501 agcgggtgcc tttgaaatta acgatctcta cccctcttcc aacagcggcg atttagaagt
     1561 cacgattgaa gaaagtgacg gtacacaacg tcgctttatc cagccttatt cttcattacc
     1621 catgatgcag cgacctgggc atctaaaata tagcgcgacc gctggacgct atcgcgctga
     1681 tgcaaacagt gatagcaagg aacccgaatt tgctgaagcc acggcaatat atggtttgaa
     1741 taatactttt acgctgtatg gcggcctgct cggttctgaa gattattatg cgctggggat
     1801 cggtatcggc ggcacacttg gcgcactggg cgcgttgtcg atggatatca acagagctga
     1861 cacccaattc gataaccagc actcttttca tggctatcaa tggcgtacgc agtacatcaa
     1921 agatatcccg gaaaccaaca ccaatatcgc tgttagctac tatcgctata ccaacgatgg
     1981 ctattttagt tttgatgaag ccaatacccg caattggggc tataacagtc gccaaaaaag
     2041 tgaaattcaa ttcaacatca gccagacaat atttgatggg gtaagtctgt atgcctccgg
     2101 ttcacagcaa gactattggg gcaataacga gaaaaacagt aatatctctg ttggggtttc
     2161 cggccagcaa tggggaattg gttacagcct gaattatcaa tacagccgct acactgatga
     2221 aaataatgac cgtgcactct ctttgaatct cagtattcc
//
LOCUS       DBKEKI010000274         2208 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.278, whole genome shotgun sequence.
ACCESSION   DBKEKI010000274 DBKEKI010000000
VERSION     DBKEKI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.278"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..433
                     /locus_tag="ACUQPX_004331"
     CDS             <1..433
                     /locus_tag="ACUQPX_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HHV9058770.1"
                     /translation="VIREYIEIFYNRQRRHSRLGNISPAAFRIKYCQYTSVLGLARSS
                     YFYHRAALKAGDKYATIRKMLTDIFNSNYQCYGYRHLHAMLRHEGGRLSEKVVRRLMV
                     EEQLVVSRNRRRRYSSYCGEIGPAPDNLIARDFKAEQPNQK"
     gene            443..910
                     /locus_tag="ACUQPX_004332"
     CDS             443..910
                     /locus_tag="ACUQPX_004332"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HHV9058771.1"
                     /translation="MTEFQFPAGKVWLSPVVDCFDGKVVSWSLSTRPDAELVNTMLDS
                     AVETLNAGERPVIHSDRGGHYRWPGWLERVNAAGLIRSMSRKGCSPDNAACEGFFGRL
                     KTEMYYGRKWSGITPEKFMQQVDAYIRWYNERRIKLSLGAVSPKMYRQQCGLE"
     gene            1010..1366
                     /locus_tag="ACUQPX_004333"
                     /pseudo
     CDS             1010..1366
                     /locus_tag="ACUQPX_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase domain-containing protein"
     gene            1349..2186
                     /locus_tag="ACUQPX_004334"
                     /pseudo
     CDS             1349..2186
                     /locus_tag="ACUQPX_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          548 a          533 c          658 g          469 t
ORIGIN      
        1 agtaatacgg gaatacattg agattttcta caatcgtcag cgtcgtcact ctcgtctggg
       61 gaatatctcc ccggcagcct tcaggataaa atattgccag tacacctcag ttctgggcct
      121 tgcccgtagc agttattttt atcaccgggc tgcactgaaa gccggtgata aatacgccac
      181 gatacgtaag atgctgacag atatctttaa cagtaattac cagtgttatg gctatcgtca
      241 cctgcatgcg atgctcaggc atgagggggg gcggctatca gaaaaggttg tacgcagact
      301 tatggtggag gaacagcttg ttgtcagtcg taaccgtcgt cgtcgctaca gctcatattg
      361 cggagaaatc ggaccggctc cggataacct tatcgccaga gattttaagg cggagcaacc
      421 taatcagaaa tagctgacag atatcacgga gttccagttc cctgcaggta aagtctggct
      481 gtcaccagtg gtggactgct tcgatggaaa agttgtgagc tggtctctca gtacacgccc
      541 tgatgctgaa ctggtcaaca ctatgctgga tagcgctgtc gaaacgttaa atgctggcga
      601 acgaccggtg atacacagtg acagaggtgg gcattatcgc tggccaggct ggctggaaag
      661 agtgaatgca gcaggtctta ttcgctcaat gtcccgtaaa ggatgttcac ctgataatgc
      721 tgcatgcgaa ggctttttcg gcagactgaa aacggaaatg tattatgggc gtaaatggtc
      781 gggcatcacg ccagaaaagt tcatgcagca agtggatgct tacatcagat ggtataacga
      841 gcggcgtata aaattatcgc tgggtgccgt cagccccaaa atgtaccgcc aacaatgcgg
      901 gctggaatga taaagcagtc caggaaatcg tccgcatccc cccttcacca tttggattgt
      961 ccgctccacc caacatgttg tttccttaag gttcctacac cacaaaggaa actctcatgc
     1021 tgcgcagtgg atggctgacg ttgccgacag gcgggaactt cgccagttca ggcagacacc
     1081 ggaacagcgc ttcacgcagg aacaggagca tctgcagccg ctgctgggta cggacttcga
     1141 tatccgtcat gtctcctggg atggctatat cgaggttggc gggaaccgtt acagtgtgcc
     1201 ggaaagcctg tgtggtcagc tggtatcaat acggatctcg ctggatgatg aactgcggat
     1261 ctacagtaat gagcaacagg tagcatcgca ccgtctctgt tcggctgcat acggctggca
     1321 gaccgtgccg gagcatcacg ctccactctg gcagcaggct tcggagcgca tggccgaacg
     1381 gctgggggag atacagaaac gggtaatcac tgtctgtgac cgggaagcgg atatctggca
     1441 ttacctgtat tataaagtca gccacggaca gcgtggtgcg tgctgcacag aatcgccggc
     1501 tggaagaggc acccggcaag ctcttcgaac tgccggaagt cctggcaacc gcaggaagcc
     1561 acacgctgaa tgtgatgcaa aaaggcgggc gggcagcccg tcaggcccgg atgttcatca
     1621 gttacagcga agtcagcata aaaaatcccg acaacagcgg ccaggcgctc ccgctcacgt
     1681 atgtctgttg ccgggagcag gcagaggacg gtgcctgctg gcatctgctg acgtcaggaa
     1741 aagcggcgag tgccgccgat gcacgacgta ttgtcagcca ttacgagcga cgctggctga
     1801 cagaggaata ccacaaggcg tggaaaagtg gtggtacatg gaatcgctgc gaatgcagac
     1861 ccgggataac cttgagcgca tggtggttat ccaggcgttt atagcggtac gggtgctggg
     1921 cctgcggcag ggaggtgtca gtgaagaaac acaaaatgac agctgtgaga aaatactgac
     1981 gccgacagag tggaaactac tgtgggttaa gctggaagga aagccgcttc cggtgcaggc
     2041 accgaccctg aaatgggcct ggggcgatgg catgacagca aacgcacagg tcgtcccggt
     2101 tggggcgtca tgtgggatgg ctggtcagac ttcaggatat ggttgagggt tacctggtga
     2161 tgaagtctct tgatcaggag ctctgatcaa gagacagcag ctttgggg
//
LOCUS       DBKEKI010000275         2169 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.279, whole genome shotgun sequence.
ACCESSION   DBKEKI010000275 DBKEKI010000000
VERSION     DBKEKI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.279"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(220..384)
                     /locus_tag="ACUQPX_004335"
     CDS             complement(220..384)
                     /locus_tag="ACUQPX_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058772.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="ACUQPX_004336"
     CDS             1286..1570
                     /locus_tag="ACUQPX_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HHV9058773.1"
                     /translation="MIYGGFMKSSVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="ACUQPX_004337"
     CDS             1558..2043
                     /locus_tag="ACUQPX_004337"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHV9058774.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          559 a          454 c          616 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgct gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgtgaga gtgtcacgta cggttccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgttt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caagtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 ggtgaagctg tacttgatga ctggttaaag cagaaggggc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagagagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggcttt
//
LOCUS       DBKEKI010000276         2145 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.280, whole genome shotgun sequence.
ACCESSION   DBKEKI010000276 DBKEKI010000000
VERSION     DBKEKI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.280"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            209..997
                     /gene="virF"
                     /locus_tag="ACUQPX_004338"
     CDS             209..997
                     /gene="virF"
                     /locus_tag="ACUQPX_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HHV9058775.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(990..1362)
                     /locus_tag="ACUQPX_004339"
                     /pseudo
     CDS             complement(990..1362)
                     /locus_tag="ACUQPX_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1361..2115
                     /locus_tag="ACUQPX_004340"
                     /pseudo
     CDS             1361..2115
                     /locus_tag="ACUQPX_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          678 a          390 c          441 g          636 t
ORIGIN      
        1 tccctttcag tgcaaatact tagcttgttg cacagagaaa tagaagctgc ataagctctt
       61 tcttcaaaaa atgtaaataa agttaaatat aggaaaaatt acttaatcta tcttaataac
      121 ggaaagattt tgtatacaat cactgttaca gaaatttctt agttactctg taaacactaa
      181 atatagtttg gtatattctg ttgaatttat gatggatatg ggacataaaa acaaaataga
      241 tataaaggtt cgcttgcata actatattat tttatatgca aaaaggtgtt caatgacggt
      301 tagctcaggc aatgaaactt tgactatcga tgaagggcaa attgctttta tagagcgaaa
      361 tatacaaata aacgtctcca taaaaaaatc tgatagcatt aatccatttg agattataag
      421 ccttgacaga aatttattat taagcattat tagaataatg gaaccaattt attcatttca
      481 acactcctat tctgaggaga aaagggggtt aaacaaaaaa atattcctcc tctctgagga
      541 ggaggtttct atcgatttgt tcaaatctat aaaagagatg cctttcggca aaagaaagat
      601 ctatagttta gcttgccttt tatcagctgt ttctgatgag gaagctttat atacttcgat
      661 atcgatagct tcttctctta gtttttctga tcagataagg aagattgttg aaaaaaacat
      721 cgagaagaga tggcgtcttt ctgatatttc aaataacttg aatttatcag aaatagctgt
      781 tagaaaacga ttggagagtg aaaaattaac atttcaacaa atccttcttg atattcgcat
      841 gcatcatgca gcaaagcttt tattgaatag tcaaagctat attaatgatg tatcaagact
      901 tatcggaata tcaagcccat cttattttat aaggaaattt aatgaatatt atggtataac
      961 tccaaagaaa ttttacttat atcataaaaa attttaaatg cttcatagcc catcgctatt
     1021 gccagatggg ttttccccac acctgatggc cccagtaata cgatattttc attacgttct
     1081 atgaagctga gtgagcgtaa cgactggagt tgcttctgcg gtgctctggt ggcgaatgtg
     1141 aagtcatact cttcgaacgt tttcaccgcc gggaaggctg ccattcgggt atacatcgcc
     1201 tgtttacgtt gatgacgtgc cagtttttct tcatgaagca tgatgctcca ggaagtccat
     1261 ataactccat tcctggtcta ctgcctgttg tgacagcgca ggcgctgcgc ttatgtactg
     1321 cacccatttt gttggacgat gaaatggaat agcccctgat atgtcaaagc caaaataccc
     1381 ttttgaaaag cgccttgaag tcgtgaatca ctacttcact actgatgatg gttacaggat
     1441 catctcggca cgttttggtg tcccccgacc ccaggtcagg acatgggttg ctctctatga
     1501 aaaacatgga gaaaaaggct taattcccaa acctaaaggt gttagtgctg atccagagtt
     1561 gcgtattaag gtcgtgaaag ctgtgattga gcagcacatg tcccttaatc aggctgctgc
     1621 tcactttatg cttgctggta gtggttctgt agccaggtgg ctgaaggttt atgaagagcg
     1681 cggagaagct ggtttacgcg cgctcaagat tggcaccaaa agaaacattg caatatcagt
     1741 tgatccagaa aaagcggcat cagcattgga gctgtcaaaa gaccgacgca ttgaggatct
     1801 tgaaaggcaa gttcgatttc ttgaaacgcg gcttgtgtat ctaaaaaagc tgaaagcctt
     1861 agctcatccc acgaaaaagt gaaagtactc aacgagctaa ggcagtttta tcctcttgat
     1921 gagcttctca gggctgcgga gataccgcgc agtacgtttt attaccatct aaaggctctc
     1981 agcaagcctg acaagtatgc ggacgttaaa aagcgtattg gtgagattta tcatgagaat
     2041 agaggccgat acggataccg tagggtaacg ctgtctcttc atcgagaagg gaaacagatt
     2101 aaccataaag ctgttcacgt ataggaaatt gaaaaactaa gcgat
//
LOCUS       DBKEKI010000277         2094 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.281, whole genome shotgun sequence.
ACCESSION   DBKEKI010000277 DBKEKI010000000
VERSION     DBKEKI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.281"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..220
                     /locus_tag="ACUQPX_004341"
                     /pseudo
     CDS             1..220
                     /locus_tag="ACUQPX_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            398..644
                     /locus_tag="ACUQPX_004342"
                     /pseudo
     CDS             398..644
                     /locus_tag="ACUQPX_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            758..1016
                     /locus_tag="ACUQPX_004343"
                     /pseudo
     CDS             758..1016
                     /locus_tag="ACUQPX_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1065..1643
                     /locus_tag="ACUQPX_004344"
     CDS             1065..1643
                     /locus_tag="ACUQPX_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058776.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1752..1983)
                     /locus_tag="ACUQPX_004345"
                     /pseudo
     CDS             complement(1752..1983)
                     /locus_tag="ACUQPX_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          667 a          358 c          514 g          555 t
ORIGIN      
        1 attactggaa gatgtggtgt acgccataac ggagacagtg agaaaaacgg cgaggcaaat
       61 cacaaggaga gggatgtatc agcagtgact gaaggttgac ccgctgaagt gcgagctgtg
      121 cggaggtcag atgcggttta cggggctgaa gaggggttac cgtctggcag agcaggttat
      181 gatgcatgag ccgctggctc gaatacggtg gtgcggctga gagtcgcaga ggggaagttg
      241 cgtccatgtt gaggggaacg gagcaaaaaa ccatcagaga tacattgaat caatcaatgt
      301 aagcaatatt aagtgtctat accgattact tgatgatcag gttgttttga ataaacgcgc
      361 attcttcggt gcttttttaa tcttttgggg atttaaattc ctatcgatga actgtcgata
      421 tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg gagaaatgtt
      481 cggagatcac gtgctggcaa cagcgatcct agaccggctg ctacacgctg aatatcaaag
      541 gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa aacacaacgc
      601 caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag tatcttagca
      661 ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg aaggagggag
      721 aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc ttgcccccaa
      781 acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga aatatgatta
      841 aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc gcgaaggtgt
      901 tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg ctgcggaaat
      961 atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa atatagagga
     1021 aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa gtctcgttta
     1081 agtctttagg ctatattttt catgatatat ataataaaaa acacactatc gatgagttca
     1141 acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat gcatgtcata
     1201 aagtggcaat ttttcttgcc gaaaaagata atgagataac aaagaaagac aaagccaaga
     1261 taatagatac attaactgaa aactattcca ttgaatttca acaattaatg aatattagtg
     1321 agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt gtcagttttg
     1381 ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat aatagcatgg
     1441 catattacca tgcaaattat agcagcattg acaaatatat tacggatatg tgcggcctta
     1501 tttgtatgag gcatattgag tctactggca ttatttttta tatgcttgat gaaaaagttt
     1561 tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt tgttcagcaa
     1621 agaacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc aacaagagtc
     1681 gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca gttgcaggtc
     1741 gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat cttgtagtgc
     1801 atatcatcag actttaagaa gcatatggtt ttgcgggtaa gccgcttaag atgtgtccgc
     1861 aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataat atgactggtt
     1921 gaggacaata aagttcggta taccggctca ggcatcggtc atgtagaagg caatgctaaa
     1981 ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg agaaaatagc
     2041 cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa cccg
//
LOCUS       DBKEKI010000278         2061 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.283, whole genome shotgun sequence.
ACCESSION   DBKEKI010000278 DBKEKI010000000
VERSION     DBKEKI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.283"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..289)
                     /locus_tag="ACUQPX_004346"
     CDS             complement(<1..289)
                     /locus_tag="ACUQPX_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate plug family protein"
                     /protein_id="HHV9058777.1"
                     /translation="MLEIVLSPVKAQQFTVTLGAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLP"
     gene            complement(289..999)
                     /locus_tag="ACUQPX_004347"
     CDS             complement(289..999)
                     /locus_tag="ACUQPX_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HHV9058778.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGGVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FTVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     VTQPSDADRRSATNRGIT"
     gene            complement(996..>2061)
                     /locus_tag="ACUQPX_004348"
     CDS             complement(996..>2061)
                     /locus_tag="ACUQPX_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HHV9058779.1"
                     /translation="ANKAAGELGGWQNVIITLIGLKVASWVRGLTKALSGPGGLLFAI
                     TALYPVVDGLLTSIVGRENKDWLDSHGFFWASDGTFFFNKKEMETYQTKLDAGEKPGN
                     ITRAQSPTVWQQGMLDTQASQATGREAAFGESWLQGMRATQEKLGNAMQNRQRPTKTG
                     EALLGWLQPKLSQLEEKYNLPPGLLRSVAITESRGNQFAVSPAGAMGLFQFMPKTASE
                     FGLRGNDAFDPEKAADAAARKLGGLIRFFHGDLAKALAAYNWGEGNVQRKGLAAAPEE
                     TRNYIPRVLANLPYPGAAMAIRSRHPAPVSQSTVTETTHIGTLNVTTTSDNVKGITDD
                     ARRRITNSALVSVYSSGVTG"
BASE COUNT          469 a          603 c          506 g          483 t
ORIGIN      
        1 caggtaacag ccagtagagc cggtaccggg atccgagccc ccgccagtca gggtcactat
       61 tcccctccgt gtcggtaaaa aacaggtccc cctgaaacgg caggtatccg taccggacaa
      121 tccggttatt attcagacac agcacgccat acaggcacgg ttcaccatta acaatgatat
      181 cgatatacat ccccgtagta cgctgattca ggcgaatggt gcagacctga gcgcccagtg
      241 tcaccgtaaa ctgctgggct ttaacgggag ataaaacaat ttccagcatc aggtgatccc
      301 cctgttcgtg gcgcttcgtc tgtcagcgtc tgacggttgt gtcaccgacg ccgtaactgg
      361 ctgcgtttta accgatgcgg ccccttttgc tttatcgttg tccgtgggag acgacttatc
      421 cgtacttccc actgacacct ctcctgtatt cattacggtc tgaaataccg cagtaaccgt
      481 cagtaaggtc ggaccattat tacttcgcat tcggtagtca tatttcacaa ggtcgcagga
      541 tgtccatgtc ttgtccggcg tctcaatatc gtagagtcct gccgtggtac gcatcctttc
      601 aagcgtttcc agcacattcg tccgcgaggt ggtggaaaaa tttgtcaggt tcgggacgga
      661 tccggaaaag gccgtccacc cctctacagt gaaagtcaca tgcagttccg gcgatctctg
      721 gattttatta aaggtggtat aagccccctg ctccacgggg acggtggaaa cagaagcctc
      781 cgcgcccacc tcaacgacaa caaaagaatc cggggagaaa ggtttgccac ccttctgatg
      841 ggcacctgcc ggatcattcc gtgcgtaata aataccgaat gacggtgcca gtacactgtt
      901 aatgagtccc aggacaccgc cgccacgaac tgcactcagt acgttacttt cactgagcga
      961 aaagttattc agggaaatat tatcgaaaga aaaactcatc ctgttacccc gctggaataa
     1021 accgacacaa gcgccgaatt cgtgatacgc cgacgtgcgt catcggtaat acccttcaca
     1081 ttgtccgacg ttgtggtgac attcagtgtc ccgatatgcg tggtttccgt tacggtggac
     1141 tgcgatacag gcgcaggatg gcgcgaccgt atggccattg ccgcacccgg ataaggcagg
     1201 ttcgccagca cccgcggaat atagttgcgg gtctcctccg gagcagcagc cagtccctta
     1261 cgctgaacat ttccctctcc ccagttatat gccgccagag ccttagccag atcgccatga
     1321 aaaaaacgta tcaggccacc aagttttctc gcggcggcat cagcggcttt ttcgggatca
     1381 aaggcatcgt tccccctcag accaaattca ctggccgtct tcggcatgaa ctgaaacagt
     1441 cccatcgcac cagcaggtga gacggcaaac tgattaccac gggattcggt gatcgcaacg
     1501 ctgcgcagca gtccgggcgg caggttatat ttttcctcca gttgagacag tttcggttgc
     1561 agccagccta acagggcttc cccggtcttc gtcggtcgct ggcggttttg catggcatta
     1621 ccgagttttt cctgcgtcgc acgcataccc tgtagccagg attcaccgaa ggctgcttcc
     1681 ctcccggttg cctgagaagc ctgagtatcc agcatgccct gctgccatac tgtgggtgat
     1741 tgtgcccggg tgatgttgcc aggcttttct ccggcatcca gttttgtctg atacgtctcc
     1801 atctctttct tattgaaaaa gaaagtcccg tctgaagccc agaaaaaacc atgtgaatcc
     1861 agccagtcct tattttccct gccaacaatg gatgtcagta atccgtcaac aaccgggtaa
     1921 agcgccgtta tcgcaaaaag aaggcctccg gggccactga gggcttttgt cagcccccgc
     1981 acccatgacg ccactttcag cccgatcagc gtaataatga cattctgcca gccccccagt
     2041 tctccggcag ccttattcgc c
//
LOCUS       DBKEKI010000279         2056 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.284, whole genome shotgun sequence.
ACCESSION   DBKEKI010000279 DBKEKI010000000
VERSION     DBKEKI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.284"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            119..712
                     /gene="tetC"
                     /locus_tag="ACUQPX_004349"
     CDS             119..712
                     /gene="tetC"
                     /locus_tag="ACUQPX_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HHV9058780.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            complement(825..2030)
                     /gene="tet(B)"
                     /locus_tag="ACUQPX_004350"
     CDS             complement(825..2030)
                     /gene="tet(B)"
                     /locus_tag="ACUQPX_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HHV9058781.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          719 a          435 c          364 g          538 t
ORIGIN      
        1 tttgatagcg agaatgctgt tcaatataca taaaataacc taaatgttct taagattgtc
       61 acgaccacat catcatgata ccataaacat actgacggta tgttatttta aatctatcat
      121 ggaaaataaa aatcatcaac aagaaaattt taagagtacc tatcaatcac tggttaactc
      181 agcacgaata ttgtttgttg aaaaaggcta tcaagctgtt tcaatagatg agatctcggg
      241 aaaagcgttg gtgaccaaag gtgcctttta tcatcacttt aaaaataaaa aacaattact
      301 cagtgcctgt tataagcagc aattaattat gattgatgcc tacatcacaa caaaaactga
      361 tttaacaaat ggttggtctg ccttagaaag tatatttgaa cattatcttg attatattat
      421 tgataataat aaaaacctta tccctatcca agaagtgatg cctatcattg gttggaatga
      481 acttgaaaaa attagccttg aatacattac tggtaaggta aacgccattg tcagcaaatt
      541 gatccaagag aaccaactta aagcttatga tgatgatgtg cttaaaaact tactcaatgg
      601 ctggtttatg catatcgcaa tacatgcgaa aaacctaaaa gagcttgccg ataaaaaagg
      661 ccaatttatt gctatttacc gcggcttttt attgagcttg aaagataaat aaaatagata
      721 ggttttattt gaagctaaat cttctttatc gtaaaaaatg ccctcttggg ttatcaagag
      781 ggtcattata tttcgcggaa taacatcatt tggtgacgaa ataactaagc acttgtctcc
      841 tgtttactcc cctgagcttg aggggttaac atgaaggtca tcgatagcag gataataata
      901 cagtaaaacg ctaaaccaat aatccaaatc cagccatccc aaattggtag tgaatgatta
      961 taaataacag caaacagtaa tgggccaata acaccggttg cattggtaag gctcaccaat
     1021 aatccctgta aagcaccttg ctgatgactc tttgtttgga tagacatcac tccctgtaat
     1081 gcaggtaaag cgatcccacc accagccaat aaaattaaaa cagggaaaac taaccaacct
     1141 tcagatataa acgctaaaaa ggcaaatgca ctactatctg caataaatcc gagcagtact
     1201 gccgtttttt cgccccattt agtggctatt cttcctgcca caaaggcttg gaatactgag
     1261 tgtaaaagac caagacccgc taatgaaaag ccaaccatca tgctattcca tccaaaacga
     1321 ttttcggtaa atagcaccca caccgttgcg ggaatttggc ctatcaattg cgctgaaaaa
     1381 taaataatca acaaaatggg catcgtttta aataaagtga tgtataccga attcgattgc
     1441 gtctcaaccc ctacttcggt atctgtatta tcacgtgtat ttttggtttc acggaaccaa
     1501 aacataacca caaggaaagc gacaatattt agcaacgcag cgataaaaaa gggactatgc
     1561 ggtgaaatct ctcctgcaaa accaccaata ataggccccg ctattaaacc aagcccaaaa
     1621 cttgccccta accaaccgaa ccacttcacg cgttgagaag ctgaggtggt atcggcaatg
     1681 accgatgccg cgacagcccc agtagctcct gtgatccctg aaagcaaacg gcctaaatac
     1741 agcatccaaa gcgcacttga aaaagccagc aataagtaat ccagcgatgc gcctattaat
     1801 gacaacaaca gcactgggcg ccgaccaaat cggtcagaca tttttccaag ccaaggagca
     1861 aagataacct gcattaacgc ataaagtgca agcaatacgc caaagtggtt agcgatatct
     1921 tccgaagcaa taaattcacg taataacgtt ggcaagactg gcatgataag gccaatcccc
     1981 atggcatcga gtaacgtaat taccaatgcg atctttgtcg aactattcat ttcacttttc
     2041 tctatcactg ataggg
//
LOCUS       DBKEKI010000280         1940 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.285, whole genome shotgun sequence.
ACCESSION   DBKEKI010000280 DBKEKI010000000
VERSION     DBKEKI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.285"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(67..336)
                     /locus_tag="ACUQPX_004351"
                     /pseudo
     CDS             complement(67..336)
                     /locus_tag="ACUQPX_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(451..1089)
                     /gene="ospI"
                     /locus_tag="ACUQPX_004352"
     CDS             complement(451..1089)
                     /gene="ospI"
                     /locus_tag="ACUQPX_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HHV9058782.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1242..1717)
                     /locus_tag="ACUQPX_004353"
                     /pseudo
     CDS             complement(1242..1717)
                     /locus_tag="ACUQPX_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          564 a          471 c          366 g          539 t
ORIGIN      
        1 aaaaactagt attaagacta tcacttattt aagtgatact ggttgtctgg agattcaggg
       61 ggccagtcta cccagcaagc gctgtaactc ttttatttgt tttatagcgg cagccagttc
      121 tgaggcagga acgacatcct ctccggcagc aacagcagtg agacttcctt ccagatatta
      181 ttttttccac ttaagcaaca ggctgggctg aataccatgc aaacgcgcca catgggacac
      241 cgtcatgcca ggctccattg tctgctgaat aatggcaatt ttttcctgag gagttttacg
      301 tttacgaagc tccagcccca gcaaagttcc agtcatctca aaattagaac tagtgttaga
      361 catatatcca agcctatctc ttattttaag atacatccac tgtctggcgt ttcaaggggc
      421 cggctcaaat ctatgtacag gctctcagca tcagcaaagc ctcttactct tccaactact
      481 actgttagca tagtgatcaa attcctgatc agatacatga attacctgaa ccgaactatt
      541 ttcatctaca aatgcatctg gcgatttttc tacaggatca taaagattaa atccctttcc
      601 tgtttgaaca tccaaaaaca taattttttc accatcgtta aatccagcta tccaatgagt
      661 ggcctgttca atagaaatca ttactacact tccttttgaa gtgttttgta aaatattttc
      721 tgcaatacgc tcacgaaatt tactataagt ttcattagga tgtgacacta tgccttcact
      781 atcaatttgc tctaaatgcc tatgaaaaaa tccggtcata tactctgata tttcattagg
      841 tacgggagcc tctcttactc cataccctaa catagccata caagcatgaa gcgcacaccc
      901 tgaacagtta ccatccccac cagacggatt tatcattttc tgtaaagtcg ctgccaattc
      961 agaatcttta tccatatcac caagactttt agagtttaaa gattccagca attttttaac
     1021 atttacattt ccctcctctg tatgtgcctc atatccagca gttccctgta acgacacccc
     1081 attaatcata ttgtatcctg acagtatata tactcactat attttcttca taacaaatat
     1141 ccctctgata tgacaacaaa aaacggagcc ggattcctgt ttttgtgaac tgcagctatt
     1201 cgagtctaaa aagtgatcca cttaccacca acggcctaat atatacagaa cggagtaaac
     1261 agggctaaca gttcccgaca ggtctcatca ttccgtggcc cgaaagtgta agccaacaca
     1321 ccgccagttt tagtgttgta cgcataccag agccagtgtt gttgcagcct tgctgccaac
     1381 gaagcgccac tgctcatcaa gttcacagat aagggcaaca tcagcgtgag cgactggcgc
     1441 agatgttatc cgccggggcg ggagcttttt aatgtccgta tgacggtatt gatgccgatt
     1501 ttcagtgtcc tggcggtatc acggccccct gcgccgttat gtgccatttc aacgatctgc
     1561 tccttaacgc cgagtttctg gcttcataag agtaagtgag ctggaaaaca cgtttacatg
     1621 agcgacaacg gaaacgctca tgccgggaac aactacggcc atgtcgataa acttcatcgg
     1681 aattacagcg cggacaatga acagtaacgg tagccatgga aaaccttaaa taccagtaga
     1741 ttaccagaaa cttctgtcga ttgaagacat cacccgaagt ttgaattcat tcgaatactt
     1801 tttacgagta agcgtacagc ctgaaccgtc tggtcagaat ctgacgaatt agacaaagtg
     1861 gtgtccacca aataagtagt gggaaccaaa gtgtcagata tgcagaaaaa tgtgactccc
     1921 ggcaggcgaa aaggctgccc
//
LOCUS       DBKEKI010000281         1938 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.286, whole genome shotgun sequence.
ACCESSION   DBKEKI010000281 DBKEKI010000000
VERSION     DBKEKI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.286"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(123..1577)
                     /gene="ospC2"
                     /locus_tag="ACUQPX_004354"
     CDS             complement(123..1577)
                     /gene="ospC2"
                     /locus_tag="ACUQPX_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HHV9058783.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYCINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          617 a          333 c          306 g          682 t
ORIGIN      
        1 ccggggcgtt caccacatgc aatgtgttgt tgcaggggaa cggtctgcac catcccctat
       61 atcgatcaat ataacaaaaa tgacaacgtc agattcaggg cacaacctaa atatatttta
      121 ggtcacctcc caaatcgttt gcctgatacc gctccatttt ttaataaaaa atcaattgtt
      181 tttgaatctt tgcttttcat tgcattgtca agcatagtgt ccccactatt tgctttttga
      241 aatcttttgt ttacatcaac caatccatta ttaatacaat attctagcac tttatagttg
      301 gcactatctt cactaagtaa atactcaaca tcatacagct cacaatacat attgttgttc
      361 attttctcca tctctgcgac atcttgtttt gtaaaattaa atttagatag taaataaagt
      421 gccatatcag attttgcatt gcctagagaa taagcaagag cttgatgagc cattttttta
      481 tcagtcacct tattgttaat ttcttcataa ttggatgctt taatggcatc ctccaaagat
      541 atatctctga gtcttatctt tttgaagtta tctgtgctta gcattcttgg tatatgatac
      601 tcaagctgaa acacaaagtt tataattcta tccagactaa caggatcaat gtttttatta
      661 taacaaaact ctcggaatct ttgatcttta ctctttctta taaaatcaat aagatgcaac
      721 ccaattccaa gcctcatatc tttagtatta aatattggca catcagtttt tccctgatta
      781 ccatgcacat acctgctaac ttcatccaca acctctttaa agttatctaa gaaaaatgat
      841 tggtgctcat ggtaaaaaac aggtggaata gcattatcaa agtggtcggt taatgtcata
      901 tatccatatg gagagtcatg atcaatgata tacgcattag caccaaaatc cacagtatga
      961 tgtgttttat ttagtgggag tgtttcttga tgattggaaa tttcaacccc aaaaaaaaca
     1021 aaatcgtggt tagctatttc ttcaatatcc tcagagaatg tgtttttata tgtttgtata
     1081 ttctcagact taattttatc aaaggactta atattaagag tattatttga caggttagcg
     1141 ttactttgat gtctgaagtt aaaatcagaa ttaataataa tagagagcaa gtgacgttca
     1201 tccgaagaaa gaggatgagt tttttgtatt atttcagaaa gatagtgtct ggacacctta
     1261 tcgaggtgct caagtgaccg aaatgatgac cgtgctccag atggcgcttc aatagaaaga
     1321 tttaatgatt tgaaactggt cccttgagag aacattttac tataactttg cgctgctata
     1381 gacttcctta aaaagctttt cacagtatca gcacagtgat ctaaatgttt ttcacttatg
     1441 ccagaagagg atgagtttgt tacgcatgat atgttttttt gtaatgcttt tgtatctttg
     1501 ttcggattta tttttccatc aacaagatca atattgttct gaacattaat atgatttact
     1561 gcttcaggta ttttcataaa acatccccca ttatgtgcgt ttttatctgc agttatttag
     1621 aataatatta tctaacttaa tattttcttg tatgtattat aacgaacata ttttaatata
     1681 ttgtacgatt ttcactctga aatcccctga gtttaatcac acttccttta ctgaaaggaa
     1741 ttattgcaac agtattattt gtttttggat atatatcttg cgccttgtta tgtagttaat
     1801 gctcagaata tgatagataa aatagagacg cagaacaggg catgctcata acatgatgat
     1861 aacataaaaa attagtaaca ataaatgaaa gaatcatggg cgctcaacaa aaagagagct
     1921 aaatagctgc gcggaata
//
LOCUS       DBKEKI010000282         1933 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.287, whole genome shotgun sequence.
ACCESSION   DBKEKI010000282 DBKEKI010000000
VERSION     DBKEKI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.287"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..783)
                     /locus_tag="ACUQPX_004355"
     CDS             complement(<1..783)
                     /locus_tag="ACUQPX_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HHV9058784.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSITTGEHIISHTASSIKRTHMELGGKAPVIVFDDADI"
     gene            complement(805..1599)
                     /locus_tag="ACUQPX_004356"
     CDS             complement(805..1599)
                     /locus_tag="ACUQPX_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9058785.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(1589..>1933)
                     /locus_tag="ACUQPX_004357"
     CDS             complement(1589..>1933)
                     /locus_tag="ACUQPX_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9058786.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT          502 a          568 c          484 g          379 t
ORIGIN      
        1 aatatccgca tcatcaaaaa caatcactgg cgctttgcca ccaagttcca tatgagtacg
       61 cttaatggac gacgcggtat ggctgatgat gtgctcgccg gtggtgatag agcccgtcag
      121 cgacaccatc cgcactttgg gatgaccggt cagcggatca cccaccgttt tgcctctgcc
      181 aaacagtatg ttaatcacgc ctgccgggaa gatatctttc gccagctctg ccaacttcaa
      241 cgcggtcagc ggggtaattt ctgatggttt aagcactacg cagttccctg ccgccagcgc
      301 cggagcaagt ttccacgcgg ccatcatcag cggataattc cacggtgcga tagaagccac
      361 gacccccaac ggatcgcgac ggatcatcga agtatgacct tcaagatatt cacctgccgc
      421 cagaccattc agacagcgcg ccgcacccgc gaaaaagcga aaaacatcga caatcgccgg
      481 gatttcatca ttgaacgcac tatgcagcgg tttgccacaa ttacgggatt ccagttcggc
      541 aaaaacctga ccattttctt cgataacatc cgccagtttc agcaggcatt ccgcacgcgc
      601 tttcggtgtg gtttgccccc attcagcaaa tgctgcatct gccgcacgca cagcagcatc
      661 gacctgctct gcggatgcct cggcaatctc cagtaaaacg tcccccgttg ccggattata
      721 gacaggctgt ttttcccctt cgccgctaac cagttctccg ttaatcagta acttatgttg
      781 catagcttat tcctgtatca gtggttattg accattgtcg ccttcgcggg ttagccacca
      841 ggcccccagg atcggcaagg ttgttaccaa cataaccagc agtgccacca cgttggttac
      901 cggtacatca cgcggtcgcc caagctgatt gagcaaccac aacggtaacg ttcgttcatg
      961 acctgccgta aaggtcgtaa cgatgatttc atcgaacgac aaggcaaacg ccagcattcc
     1021 tcctgccagt aacgccgaac tgagattcgg caacactacg tagcggaagg tttgccagcc
     1081 attggcccca agatccattg acgcctcaac cagactccac gaggtgcggc gaaaacgggc
     1141 gatgacattg ttaaacacca ccactacgca aaaagtcgca tgaccgacca cgatggtgaa
     1201 aaatcccggc tccagattga tggttttaaa ggcggttaat agcgccagac cagtgacaat
     1261 gcccggcagc gcaatgggca gcagcaataa cagcgaaatg gcgtttttgc cgaaaaagtc
     1321 tcgtctccac agcgcggcag ctgccagcgt ccctaacacc agcgcaatta atgtcgccag
     1381 cgccgccact ttaagtgaca gcgtcacggc atcaagaata tcactacgct gtgctgccac
     1441 gctaaaccag cgcagcgtca ggccctgcgg tggaaaacta aacgccgcat cttcagtgtt
     1501 aaacgcatag gcggcgatta tcaggatggg aaaatgtagg aaaacaacgc cgccccaggc
     1561 cgccagtttg aggaaaaacg gagcgcgttc agagtgcatc gaacgctccc agacgtttca
     1621 cgaacgccag gtacagtgcg atgagaataa tcggcaccag ggtgaatgcc gccgccatcg
     1681 gcatattgcc aatcgccccc tgctgggaat aaaccatatt gccgataaaa tatcctggag
     1741 gaccaaccag ctgcgggacg ataaaatcgc ccagtgtgag tgagaaggta aagatagagc
     1801 cagcggcaat acccgggatt gccagcggca gcaccacata gcgaaaggtt tgtcgtggac
     1861 gtgcgccgag atcagccgac gcctgcaaca atgacggcgg caaacgctca agcgccgcct
     1921 gaacgggcag gat
//
LOCUS       DBKEKI010000283         1929 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.288, whole genome shotgun sequence.
ACCESSION   DBKEKI010000283 DBKEKI010000000
VERSION     DBKEKI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.288"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..1212
                     /gene="dgcM"
                     /locus_tag="ACUQPX_004358"
                     /pseudo
     CDS             1..1212
                     /gene="dgcM"
                     /locus_tag="ACUQPX_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(1233..1796)
                     /gene="smrA"
                     /locus_tag="ACUQPX_004359"
     CDS             complement(1233..1796)
                     /gene="smrA"
                     /locus_tag="ACUQPX_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HHV9058787.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKAAQAKQENWERHAKRSR"
BASE COUNT          479 a          494 c          464 g          492 t
ORIGIN      
        1 taccctggac ttactcacca gtcctgtctg gattgtttcg ccctttgagg aacagttaat
       61 ttatgccaat agcgcggcgc gactgttgat gcaagacctc acgtttagtc agctacgaac
      121 cggaccctat tccgtctcct cacaaaaaga actgccgaaa tacctctccg atctgcaaaa
      181 ccaacacgat attatcgaaa tcctcactgt tcagcgtaaa gaagaggaaa cagcattaag
      241 ctgtcggctt gttttgcgag agctgacaga aacagaaccg gtgattattt tcgaaggtat
      301 cgaagcgccg gcaacgctgg gtttaaaagc cagtcgctcg gcaaattatc agcgcaaaaa
      361 acaaggtttt tatgcgcgct tttttctgac taactctgca ccaatgttgt tgattgaccc
      421 gtcacgagat ggacaaatcg tcgatgttaa cctcaccgcg ctcaatttct atggttataa
      481 ccatgaaacg atgtgccaga aacatacctg ggaaataaat atgcttgggc gtcgcgtcat
      541 gcctatcatg catgaaattt cgcatttacc cggcggtcat aaccctttga attttgttca
      601 taaactggcg gatggatcta ctcgtcatgt gcagacctat gccggaccga ttgaaattta
      661 tggcgacaag ctcatgttat gtattgtgca tgatattact gagcaaaaac ggctggagga
      721 gcagctggaa catgctgctc accatgacgc gatgaccgga ttactgaatc ggcgacagtt
      781 ttatcacatt acggaaccag gccaaatgca gcatctcgcc atcgctcagg attacagctt
      841 gttgctcatc gacaccgatc gttttaaaca cattaacgat ctctatgggc attctaaagg
      901 gatgaggtgt tatgcgccct cgcccgcccc ctcgaaagtt gcgctcgcaa aggcgatttg
      961 gtgtttcgtt ggggaggcga agagtttgtc ttattgctac caagaacccc actggatacc
     1021 gcgctttcgc tggctgaaac tatccgcgta agcgtggcaa aagtgagtat ttcgggctta
     1081 ccacgcttta ccgtcagcat tggtgtggcg catcacgaag gaaatgaaag catcgatgaa
     1141 ctgtttaaac gcgttgatga tgctttgtat cgggcgaaaa atgatggacg caaccgcgtg
     1201 ctggcggcat aagccgcgga tgcgtctcga gatcaacgac tgcgcttagc gtggcgctcc
     1261 cagttttctt gcttcgcctg cgccgcttta cgtagtgcga cgtaacacgc cccgctgccg
     1321 ccatgatgcg gtagcgcggt gcaatatgcc tgaacatcat caaattcggt cagccagcgc
     1381 gccacatagc tgcggacaat attggcatgt gatttatctt cccgtccttt accatgaata
     1441 atcagcacat tacgcaaacc atccgccagg gcttgttgaa tgaaactgaa caccattttg
     1501 cggcactctt ccaccggctg gcgcaaaaga ttcaggctcg cctgttgcgg atatttacca
     1561 ctgcgcagct tatccagcac cccatgctgc aacccttcct gccgaaactc cagcggctga
     1621 cttagcggga tgatgtcgag aaatccggta gtgaggaaat tatcaagctg cagcgtgtcg
     1681 atacgctgcg gcgcacgttg gttacgcgtt ggatgccagt ggacatctgt agcacgtttc
     1741 agcggctgga catcttccat ggcgtcaaga aacagcgatt tgtcgtcaag gttcatgtaa
     1801 cctcctatgc accttcacac gtcgtgattc agacgggtta attgttcata cttactgcat
     1861 cgttcccttc acagattgaa acaatactct acttcatacc tgtgagtatc tgcgcaggcg
     1921 gggcatttt
//
LOCUS       DBKEKI010000284         1918 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.289, whole genome shotgun sequence.
ACCESSION   DBKEKI010000284 DBKEKI010000000
VERSION     DBKEKI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.289"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..849)
                     /locus_tag="ACUQPX_004360"
     CDS             complement(<1..849)
                     /locus_tag="ACUQPX_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HHV9058788.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIHSLTPFSLPMDLTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
     gene            complement(1238..1504)
                     /gene="ospE2"
                     /locus_tag="ACUQPX_004361"
     CDS             complement(1238..1504)
                     /gene="ospE2"
                     /locus_tag="ACUQPX_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HHV9058789.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(1613..1794)
                     /locus_tag="ACUQPX_004362"
                     /pseudo
     CDS             complement(1613..1794)
                     /locus_tag="ACUQPX_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          527 a          320 c          410 g          661 t
ORIGIN      
        1 gtgttctgga gcggaaattg tggcagaatt tcccatagag aaaaatattc gaggaccaga
       61 ataatctggt ccggtggtta tttgttgtag tgtacgcatg gtatggccag acaatggatt
      121 acctgcaaca tttacgaagg cattctgagc taatctcagg acactttccg gaagtgttgt
      181 cagattatta ttcattagca cagccctgtt catactaaaa ggtaattccg gtagttgttc
      241 tataaaatta ttagccagag ccagcccttc aagcttatct ggaaaggggg gaatgctgtc
      301 taatctgttt ccagaaacta ccagtaattt tagttccggt ggcaatagtg gcagaatatg
      361 caatcggtta ttatgaaccc gtaaatgagt tagattttca ggaagaaatg gtaattcttt
      421 tatcttgtta tcacccgcat taagctcttc taacgtctcg ggtaaggcag gtaagctttc
      481 cagttgatta aattgagcat gtattacctt aagcattggt ggtaaatcag ggagatgtgt
      541 taataggttt tttcttattt ccagtgttgt tatctgaggc ggtaaatctg gtaatgtttt
      601 taaatctaac tcagataaat ttaattctgc ctcttgattt tgtaaacata ttttcagcct
      661 attgaaggcg atattgcgtt gctcgcctgg ggtaccgttc ttttcccatt cactccaggc
      721 ttttaaataa aattcattac aagatggagt cagatccata ggtaaagaaa atggagttaa
      781 cgagtgtata ttgtttattt gatgcataat accagaaccg attgcctgta tattggttga
      841 tttaatcatt ataactagaa ttcttatcaa cgataagaat tctagtgaaa tagaatatag
      901 cttattaatt ttaaaacagt ttgctttgta ccctttctcc gggtattgct ttccccctaa
      961 gccgtctatg ggaaggagtc tgctgttatt cctgagaatg gtaattcagc cataaaaagg
     1021 tatttaaggt cattgcgtct ctccctgtac tcgaataatg gtgaagaaag catgatgaat
     1081 atatcacgat aacgttcagt acgtatcgag gataaatgga gttactcctc tctgaaatag
     1141 aattactgct ctattttttt ctgttttcag aagttattag taacaaaccc tgatatattt
     1201 ttttgaaaat ggagcttaaa cgagtctgaa attaacttca gaaatcagaa taccgttggc
     1261 tttgtttttc atcaattccg gcttcattta tctttgtcaa ccattgcatt tctgtagcag
     1321 tgaatttcaa cgttttcgtc cggttggcag caggtatttg taattcgtat atagctgctt
     1381 ttttattgaa aacagtatat ccatctgtag taacagtaga agataataat acaggatttg
     1441 atacttcaag gatgatattt tcttgttttt gtggaaggca agggaatata gtttgtgtaa
     1501 gcatattact tccttatgtt atattttggt aaaaaattta tttttttaca ttatagtcgg
     1561 ctaaatgaag tttactgtaa aaaaacgata ctttttcggg tggcgcttcc aatcagtaga
     1621 atatgtgttt ttcaatgaag gagccgatga cttttcgtgg atttccacgg aacgcgagaa
     1681 gcagattgtt ttacgtgcca gccgcttgat gcgggtgcgg agggtcaggt tgttacgttc
     1741 aagatatagc atgtgctgga tgaagatccc ttttctggtc acctgtttat ctttcgtggc
     1801 cgacggggtg tgaaccgccc cgggtttcct ggagagtgtt ttatctgtga actcaggctg
     1861 ccagatcatc gtttccgatg gaagcataat aagctttttc tgcttctgcc ggagggat
//
LOCUS       DBKEKI010000285         1874 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.290, whole genome shotgun sequence.
ACCESSION   DBKEKI010000285 DBKEKI010000000
VERSION     DBKEKI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.290"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..430
                     /locus_tag="ACUQPX_004363"
     CDS             <1..430
                     /locus_tag="ACUQPX_004363"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HHV9058790.1"
                     /translation="GSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGV
                     THRFVGTEPFCRVTAQYNQDMRYWLETPTISAAPIELVEIERLRYQEMPISASRVRQL
                     LAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
     gene            445..741
                     /gene="citD"
                     /locus_tag="ACUQPX_004364"
     CDS             445..741
                     /gene="citD"
                     /locus_tag="ACUQPX_004364"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HHV9058791.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            738..1646
                     /gene="citE"
                     /locus_tag="ACUQPX_004365"
     CDS             738..1646
                     /gene="citE"
                     /locus_tag="ACUQPX_004365"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HHV9058792.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            1657..>1874
                     /locus_tag="ACUQPX_004366"
     CDS             1657..>1874
                     /locus_tag="ACUQPX_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HHV9058793.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRG"
BASE COUNT          432 a          557 c          489 g          396 t
ORIGIN      
        1 cggctcagaa tacatcatct cccgcgctac gttcccttgc tacttcatta aagaacagag
       61 cgtcattaac cattgttaca ccgaaattga tctgaagatt ttccgtcagt acctcgctcc
      121 cgcgcttggt gtaactcacc gctttgtcgg tactgaaccc ttttgtcgcg ttaccgccca
      181 gtacaaccag gatatgcgct actggctgga aacgccgact atctccgcag cgcccatcga
      241 actggttgaa attgagcggc tgcgttacca ggagatgccg atatccgctt cccgggtacg
      301 tcaactgctg gcgaaaaacg atctcacggc tatcgcgccg ctggtccctg cagtcacgct
      361 gcattatttg cagaacctgc ttgagcactc ccgccaggac gcggcagctc gtcaaaagac
      421 ccccgcatga gaaacaggtg aaaaatgaaa ataaaccagc ccgccgttgc aggcaccctt
      481 gagtctgggg atgtgatgat acgcatcgcc ccactcgata cgcaggatat cgacctgcaa
      541 atcaatagca gcgttgagaa acagtttggc gatgcaattc gcaccaccat tctggacgtt
      601 ctcgcccgct acaacgtgcg cggcgtacag ctgaatgtcg atgacaaagg cgcactggac
      661 tgcattttac gtgcacgact ggaagccctg ctggcacgcg ccagcggtat cccggctctg
      721 ccatgggagg attgccaatg atttccgctt cgctgcaaca acgtaaaact cgcacccgcc
      781 gcagcatgtt gtttgtgcct ggtgccaatg ccgcgatggt cagcaactcc ttcatctacc
      841 cggctgatgc cctgatgttt gacctcgaag actccgtggc attacgtgaa aaagacaccg
      901 cccgccgcat ggtttatcac gcgctgcaac atccgctgta tcgcgatatt gaaaccattg
      961 tgcgtgtcaa cgcgctggat tccgaatggg gtgttaacga cctggaagcc gtcgttcgcg
     1021 gtggtgcgga cgttgtgcgt ctgccgaaaa ccgataccgc tcaggatgtt ctggatattg
     1081 aaaaagagat cctgcgtata gaaaaagcct gtggtcgtga acccggcagc accggcctgc
     1141 tggcggcgat tgaatctccg ctgggcatta cccgcgcagt ggaaatcgct cacgcttccg
     1201 agcgtttgat cggtatcgcc ctcggtgcag aagactatgt gcgcaacctg cgtacagaac
     1261 gctccccgga aggaactgaa ctgctgttcg cacgctgttc cattttgcag gccgcgcgct
     1321 ctgcgggtat tcaggcgttc gataccgtct attccgacgc taacaacgaa gccggattcc
     1381 tgcaagaagc cgcccacatc aaacagctgg gctttgacgg caaatcgctg atcaacccgc
     1441 gtcagattga tctgctgcac aacctctacg caccgaccca gaaagaagtg gatcacgccc
     1501 gccgcgtcgt agaagccgct gaagccgccg ctcgcgaagg cctcggcgtg gtttccctga
     1561 acggcaagat ggtggacggt ccggttatcg atcgcgcccg tctggtgctc tcccgtgcag
     1621 aactttccgg catccgcgaa gaataaggca atcaaaatga cgcagaaaat tgaacaatct
     1681 caacgacaag aacgggtagc ggcctggaat cgtcgcactg aatgcgatct tgccgctttc
     1741 cagaactcac caaagcaaac ctaccaggct gaaaaagcgc gcgatcgcaa actgtgcgcc
     1801 aacctggaag aagcgattcg tcgctcaggt ttacaggacg gcatgacggt ttccttccat
     1861 cacgctttcc gtgg
//
LOCUS       DBKEKI010000286         1853 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.291, whole genome shotgun sequence.
ACCESSION   DBKEKI010000286 DBKEKI010000000
VERSION     DBKEKI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.291"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..246
                     /locus_tag="ACUQPX_004367"
     CDS             <1..246
                     /locus_tag="ACUQPX_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HHV9058794.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACUQPX_004368"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACUQPX_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HHV9058795.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACUQPX_004369"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACUQPX_004369"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HHV9058796.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
BASE COUNT          507 a          467 c          464 g          415 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtcgga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aat
//
LOCUS       DBKEKI010000287         1831 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.293, whole genome shotgun sequence.
ACCESSION   DBKEKI010000287 DBKEKI010000000
VERSION     DBKEKI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.293"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            149..301
                     /locus_tag="ACUQPX_004370"
                     /pseudo
     CDS             149..301
                     /locus_tag="ACUQPX_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
     gene            295..1074
                     /locus_tag="ACUQPX_004371"
                     /pseudo
     CDS             295..1074
                     /locus_tag="ACUQPX_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1288..1491
                     /locus_tag="ACUQPX_004372"
     CDS             1288..1491
                     /locus_tag="ACUQPX_004372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058797.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQIPGNKVSRCN"
     gene            1509..1829
                     /locus_tag="ACUQPX_004373"
     CDS             1509..1829
                     /locus_tag="ACUQPX_004373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058798.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
BASE COUNT          547 a          357 c          520 g          407 t
ORIGIN      
        1 ctttatcacg caggaatttg cttaaggata aagcttatag aaaccccata taatccagca
       61 ctctgaagta atgcgggcgc acaacttgcc ctcatataga cgcagggaat acaagctgtt
      121 gctggaaata ccatagcaga gcgagcgcag gatgtatctt tacgactcgg agcaggcgta
      181 caccccggtg gcgagcgtga cggggcgctg agaaagccga aaaatctggt attactacac
      241 agacctgaca ggcacggtgc aggaggtgac cgcgtcggac gggacgctgg tgtgggctgg
      301 ctgaccagcc gcacgggtca gatgtacgac catgaccatt attattacga caaagcgggc
      361 aacctgctga cggacgagta tcagagcgcg gtgatggata accgcctgcc gggctacggg
      421 cgtgaccgtt accgctataa cgaatggggc gaactgacag agcgcaggga ccagcaactg
      481 gagtggaacg cgcagggcca gttaacgcgg gtcatcagca gcaacagcga tacgcggtat
      541 cagtatgatg cgctgggcag gtgcatcagc aaggcgacat atggccgcca tacggaccgg
      601 ggagaacgaa gccggacgac gtttgtgtgg gaagggttcc ggctgttgca ggagacaacg
      661 tggcagggaa agcggacata cctgtacgac gcggaacagc cgtacacgcc ggtggccgcc
      721 atcacggggc gcggagagag ccagaaaatc tggtattacc acacggacct gacgggcacg
      781 gtacacgagg taaccgcgcc ggacgggacg ctggtgtggg cgggctacca ggcgggattt
      841 ggtgaaaacc ggggcgacat cagcaacagc ggggcgtact ttgagcagcc gcagcggctg
      901 ccggggcagt actttgacga agaaacaggg ctgcattaca atctgttcag gtattatgcg
      961 ccggagtgcg ggaggttcgt aagccaggac ccgataggat taaacggtgg gttgaactta
     1021 tatgcttatg cgcctaatcc gttaggttgg atcgatccgc taggactgaa ttctttagga
     1081 atagattcaa ataatatatt tagaggagat ggtaactata aaggtggcgg aattggacag
     1141 ccaaagagtg gcacaatatc agcacaggac attattgatc atctctaaga aaaaggtgat
     1201 ggtcgtttaa catcttttag tactaaaaaa acatctagtt ccaactctcg tggtgcagat
     1261 tttttctcaa aaaaccatgt cttcaaagtg gcagtttctg atctcgatga tttagttaaa
     1321 aataatcaga tcactgtttt aacacaagaa aaagtaaaag caatatttgg tttagaatca
     1381 agcaagaaaa ttagaaaaga acttggtaat attcttcaga atatggctag gaataatgag
     1441 gtgttagttc atgggcaaat tcctggaaat aaagttagcc gatgtaatta gttagtggag
     1501 aggaagttgt ggacgaaatt agaataagaa ttgctggtaa tacggctgcg ccttgtttcc
     1561 atgtgctaaa aaataaggga tataaagtaa caatggaaac tcatgcatta tctgacgatt
     1621 gtttagattg tgtatatgat tggtatgcgg aaaaaaatgg acaatttttt agtgcaacga
     1681 gtccagaaga gttacttgga ttagttgcaa tgtgggaagt tcgtggtaat gactggagag
     1741 ctaatttgaa tgagataaat acttatcaaa aagccataga agaatccccg ttttttgata
     1801 aaaacggcaa tgaaatagag tctgactaaa t
//
LOCUS       DBKEKI010000288         1736 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.294, whole genome shotgun sequence.
ACCESSION   DBKEKI010000288 DBKEKI010000000
VERSION     DBKEKI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.294"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1091
                     /gene="gsk"
                     /locus_tag="ACUQPX_004374"
     CDS             <1..1091
                     /gene="gsk"
                     /locus_tag="ACUQPX_004374"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HHV9058799.1"
                     /translation="AEALYQELKQKNLITHQFAGGTIGNTMHNYSVLADDRSVLLGVM
                     CSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESGERTFAISPGHMNQL
                     RAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKKYNVPVVLTLGTKFV
                     IAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALDWVDLVLCTAGPIGL
                     YMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPLRVYSHIAPYMGGPE
                     KIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYSSLAQVCKYANRVSY
                     QVLNQHSPRLTRGLPEHEDSLEESYWDR"
     gene            complement(1224..>1736)
                     /locus_tag="ACUQPX_004375"
     CDS             complement(1224..>1736)
                     /locus_tag="ACUQPX_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HHV9058800.1"
                     /translation="KYLAKTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLL
                     GEKLLASDIPLVVIETSRTRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIP
                     NGYEAGEIVASARAKNPDIEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLE
                     TPPAGEVVTG"
BASE COUNT          400 a          481 c          438 g          417 t
ORIGIN      
        1 tagccgaagc gctttatcag gagctaaaac agaaaaatct gattacccat cagtttgcgg
       61 gtggcaccat tggtaacacc atgcacaact actcggtgct cgcggacgac cgttcggtgc
      121 tgctgggcgt catgtgcagc aatattgaaa ttggcagtta tgcctatcgt tacctgtgta
      181 acacttccag ccgtaccgat cttaactatc tacaaggcgt ggatggcccg attggtcgtt
      241 gctttacgct gattggcgag tccggggaac gtacctttgc tatcagtcca ggccacatga
      301 accagctgcg ggctgaaagc attccggaag atgtgattgc cggagcctcg gcactggttc
      361 tcacctcata tctggtgcgt tgcaagccgg gtgaacccat gccggaagca accatgaaag
      421 ccattgagta cgcgaagaaa tataacgtac cggtggtgct gacgctgggc accaagtttg
      481 tcattgccga gaatccgcag tggtggcagc aattcctcaa agaccacgtt tctatccttg
      541 cgatgaacga agatgaagcc gaagcgttga ccggagaaag cgatccgttg ttggcatctg
      601 acaaggcgct ggactgggta gatctggtgc tgtgcaccgc cgggccaatc ggcttgtata
      661 tggcgggctt taccgaagac gaagcgaaac gtaaaaccca gcatccgctg ctgccgggcg
      721 ctatagcgga attcaaccag tatgagttta gccgcgccat gcgccacaag gattgccaga
      781 atccgctgcg tgtctattcg cacattgcgc cgtacatggg cgggccggaa aaaatcatga
      841 acactaatgg agcgggggat ggcgcattgg cagcgttgct gcatgacatt accgccaaca
      901 gctaccatcg tagcaacgta ccaaactcca gcaaacataa attcacctgg ttaacttatt
      961 catcgttagc gcaggtgtgt aaatatgcta accgtgtgag ctatcaggta ctgaaccagc
     1021 attcacctcg tttaacgcgc ggcttgccgg agcatgaaga cagcctggaa gagtcttact
     1081 gggatcgtta agttatcgtc ggttcgtagg ccagataagg cgttcacgcc gcatctggca
     1141 tttggctctc gatgcctgat gcgacgctgg cgcgtcttat catgcctaca tatttttcat
     1201 attttacatc cggcaaccac cgtttacccc gtcaccactt cacccgccgg tggcgtttcc
     1261 agcagttcca gcatagtacg ggcaatttca cgttcaccca tcactacctg attcgcgcca
     1321 cgttcggtga tatacgtcac ttcatcgtca taatgggcgc gggcgataat ctcaatatcc
     1381 gggtttttcg cgcgggcaga cgccacaatc tcacccgctt cataaccgtt ggggatcgtc
     1441 aggatcagcc attttgcaca ttccagatgt gccagttgca taatttcttc gttcgctgca
     1501 ttgcccaata cagcgcggac cccacgctct cgcagctcat caacacgggt tcgtgacgtc
     1561 tcaatcacca ccagcggaat atcagaggcg agcaatttct cccccagcag gctgcctaca
     1621 cgaccgtaac ccaccaatag cgcatggttg caaatatcca ctgggatctg tttctcttct
     1681 tcgattgcct cttccagcgt ctgctcttcc agcgtttcgg tcttcgccag atattt
//
LOCUS       DBKEKI010000289         1684 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.295, whole genome shotgun sequence.
ACCESSION   DBKEKI010000289 DBKEKI010000000
VERSION     DBKEKI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.295"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            240..509
                     /gene="yacA"
                     /locus_tag="ACUQPX_004376"
                     /pseudo
     CDS             240..509
                     /gene="yacA"
                     /locus_tag="ACUQPX_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
     gene            509..678
                     /gene="yacB"
                     /locus_tag="ACUQPX_004377"
                     /pseudo
     CDS             509..678
                     /gene="yacB"
                     /locus_tag="ACUQPX_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="ACUQPX_004378"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="ACUQPX_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HHV9058801.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            complement(1419..>1684)
                     /locus_tag="ACUQPX_004379"
     CDS             complement(1419..>1684)
                     /locus_tag="ACUQPX_004379"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058802.1"
                     /translation="CPLQNDGHYFCGTGGNRPDGLNEPVTFQVSLAGNPCVIRLAAQV
                     CMWLKFIIRDRGGFSGGLLLFLPVCCRDRTERILAVHTIKILR"
BASE COUNT          496 a          351 c          393 g          444 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgct gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tgtatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg accaggttgc ggcaggccgt cagtaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gccgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 gcaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cact
//
LOCUS       DBKEKI010000290         1669 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.296, whole genome shotgun sequence.
ACCESSION   DBKEKI010000290 DBKEKI010000000
VERSION     DBKEKI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.296"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1205)
                     /locus_tag="ACUQPX_004380"
     CDS             complement(<1..1205)
                     /locus_tag="ACUQPX_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HHV9058803.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSHSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNS"
BASE COUNT          427 a          369 c          367 g          506 t
ORIGIN      
        1 tggctgttgg tcatggcata aatacctctg gctcctgccg cgccggtgat agccaaactg
       61 tctccggtta ttctaccgcc gctgagcgcc cacaaaccca gcgccagact gccattacga
      121 tcaaccgtaa tatcggtatt ttgcatgttg ataacagccg ttgccgtctg ggccgaggca
      181 ccataagaac cgccggaaaa gatgctgttt cgttgcgctg ccgtgccaga aaaattgatt
      241 gtcgcgtctg aaccactggt gacgaggcca ccgccctgcg cggaagaaat atggctatct
      301 gcaccgatag tggttgtacc accacgaact tcgacgccat tggccgcggc tccagttaca
      361 tcaacagtga gtgcattcgc gctaacttgg ccaaagctcc agaggccgtg agcattatcg
      421 ccattggttt taatggtact gtttgttccg agatcgacaa cagagttttt ctgtacgttt
      481 atccccatgg cgctgtagcc ctgaacatcg attgtcaggt ctgtcgccgt aaaacgcgca
      541 gcaccattgg cgttattgcc gttgagacca ccgatataaa cacctgtact tccatcggtc
      601 ttgattttac ttccgcttcc aagatcgaca ctggtgccat agtcattgat ggttaggcct
      661 ataccgttcg agttttcaat ggtgaattga gtggctgtta gtgttgagct atgcccaata
      721 atgatgccgt catcgttact cttaatggtg ctgcctgtgc ctaagtcagc atgggtatag
      781 tcaccaatta agttaatgcc gatggcagag gtttgaccaa caacatctac tgtgagtcgg
      841 ttagctgtca gggagctgtt tttgccctga ataatgattc cttttgcata tccagaggcg
      901 tcgttgacat tgacggtgac atcattaccc aggttgatat tgccaggcgt gtttcctgct
      961 ggggtcatta caccatagag gtttcctgat ggatcggcgg tatctcctgt aattctgtca
     1021 ccatcattaa tagtaatatt ggtgccatat tgacttcctg tatcagcagc tatcagcagg
     1081 ggggaaaaga taattgcatt caaaccaata gcgccaaatt ttatcagttg aattgagtgc
     1141 gaacggattt ttaattcagc gagacgatcg gctttggtat tcccattgtg ttgtttgatg
     1201 cccataccac tcctatatag tacccaggct atgggtctat ggattttgtc attttgttta
     1261 agtcaatgaa taatatcctc ttatcatcat gatatctgta tggtgcaatc atgatgagct
     1321 tctctgagaa taattctcta ttaccgggca aaatcaggaa cacaggaatt aatatatatt
     1381 taacaccccc cttacaaagg tgagtttata tcgagttatt ccctaagttg tcgttactta
     1441 ttccaattcg ttactatgaa tatggcaaaa atattacaac aaacagcggt taaaaaattt
     1501 ggtgaagatt ttttgtaata atcctaatta gttatgggat tttcgcttga tgtatctgca
     1561 taacaaaaac aatatgcgcc atttttgcat tactaataag aaaaagcaaa aaacatcgat
     1621 attcattttt ggtcaatgga ttttctggcc tatcgttatt attgtgcta
//
LOCUS       DBKEKI010000291         1662 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.297, whole genome shotgun sequence.
ACCESSION   DBKEKI010000291 DBKEKI010000000
VERSION     DBKEKI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.297"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..738)
                     /gene="ycdX"
                     /locus_tag="ACUQPX_004381"
     CDS             complement(1..738)
                     /gene="ycdX"
                     /locus_tag="ACUQPX_004381"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HHV9058804.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHLVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(793..>1662)
                     /gene="ghrA"
                     /locus_tag="ACUQPX_004382"
     CDS             complement(793..>1662)
                     /gene="ghrA"
                     /locus_tag="ACUQPX_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HHV9058805.1"
                     /translation="PQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAVFALGAGV
                     DSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQQNSSH
                     WQPLPEYHREDFTIGILGAGVLGCKVAQSLQTWRFPLRCWSRTRKSWPGVQSFAGREE
                     LSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAALD
                     SGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVEYISRTIAQLEKG
                     ERVCGQVDRARGY"
BASE COUNT          423 a          439 c          393 g          407 t
ORIGIN      
        1 ttaaaggtct gcaaattccg caatcggtgc cataccgcga gattcaagga agttcagtaa
       61 gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa tctaccgcgt cgaggatttt
      121 aagacactct tcaaattccc ccatggtaaa cgcggtgtga gaatccgagc ctaatgccac
      181 ccaacctcca gcatcacgta ccgctgcagc taccgcacga cagttgtctt cactgccctt
      241 acgtgagtgt aaaaatgagg aattattgat ttccagcgcc accagatgtt tcgcggctgc
      301 ttcagcaacg gctttaacat ctatttcata tttcggattt ccgggatggc ttattatatg
      361 cacattgccg ctggcgatag tggcgatcat cgcttgtgta ttggtcgctt tgtcatgtgg
      421 cgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc agcgagtcaa acattttacc
      481 gctgcagtca atttcaccat caacattttt aatgttagct tcaatgccgc gcaggatccc
      541 taccccatca accactcgcg gccagatacg catgttaatg aagtgccagt gatgcggcgc
      601 atcttccata tccgggccat gatcggtgat cgcaaaaagt ttaatgccct tttgtttggc
      661 ctgggcaatg taatcactca atgtgctata tgcatgtgtg ctggcaacgg tatgcatatg
      721 aaggtcgacg ggatacataa ctctctcctg tattcaattc ccgccaagga tagcaggaat
      781 cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc ccgcagaccc tctccccttt
      841 ttcgagctgg gcaatagtgc gagaaatgta ctccacagct tcagcgggac gggtaatcgc
      901 ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag agcggacttt caggcggtaa
      961 gggttcacga ttaaaaacat ccagcattgc gcctttaact ttcccgctat ccagcgccgc
     1021 gagcaggtca tcttccacaa catgaacacc acgcgccagg ttgaggagat acgcgccatc
     1081 aggtaatttt tcgagtaatt gttgattaat aatgccgacg gtttcagggg tattcggtaa
     1141 caaattaatc aatacccgac attggctcag aaatgcagac agttcttccc gtccggcaaa
     1201 gctttgcacg ccaggccacg atttacgggt tcgactccag caacgcagcg gaaagcgcca
     1261 ggtttgcaga ctctgagcaa ctttacagcc cagtacgcct gcgcccaaaa tgccgatggt
     1321 aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc gaactatttt gctggatgcg
     1381 ataatcgtca aaacgtcgaa accaatgcag cacctgactg acagcatatt cctgcatttg
     1441 ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca gaagggttca gcatttcagg
     1501 gtgtgcctgt agcttgctca aaatagaatc aacaccggcc ccgagtgcga acaccgcttt
     1561 aagatcgcgc cctgccagca tttcaacagg aggatgccag actaaagcat aatcagcaga
     1621 gtcattatct ccgcttttcc atgccctgac tcttgcctga gg
//
LOCUS       DBKEKI010000292         1642 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.298, whole genome shotgun sequence.
ACCESSION   DBKEKI010000292 DBKEKI010000000
VERSION     DBKEKI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.298"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            261..1400
                     /gene="arnB"
                     /locus_tag="ACUQPX_004383"
     CDS             261..1400
                     /gene="arnB"
                     /locus_tag="ACUQPX_004383"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HHV9058806.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1404..>1642
                     /locus_tag="ACUQPX_004384"
     CDS             1404..>1642
                     /locus_tag="ACUQPX_004384"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HHV9058807.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYG"
BASE COUNT          446 a          402 c          386 g          408 t
ORIGIN      
        1 tgagcgacat ttctggcgat aattagcagt tggtgtaata ttaaaaatcc tacgatgtcg
       61 gcaaaatgcc tcaaattttt gccaaatgca aagcctaaat aagaaaaaat ataaaaattt
      121 caatatttac gtctaatatt agtttcttaa ggttaagtta atattctatc cttaaaattt
      181 cgctccaaat ggcaaaatat acacaacact ctttatagca aatataagtg gacaggtatt
      241 caatggcgga aggaaaagca atgtcagaat ttttgccttt ttcgcgacca gcaatgggcg
      301 tggaggaact cgctgcagtt aaagaggttc tcgaatccgg ttggatcaca accggtccga
      361 agaatcaggc gcttgagcaa gccttttgcc agttgacggg aaatcagcat gccatcgcgg
      421 tcagttcagc caccgccgga atgcatatca cgctaatggc gttggaaatt ggcaagggcg
      481 atgaagtgat tacgccttcc ctgacctggg tttcaaccct caatatgatt tccttgctgg
      541 gtgcaacgcc ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg cctgaagcga
      601 tcgaatcagc cattacgcca cgcactaaag ccatcattcc ggtgcattat gccggtgcgc
      661 cagcagatat tgacgccatt cgcgccattg gcgaacgtta cggcatcgca gttatcgaag
      721 atgctgccca tgccgtcggt acgtattaca aagggcgaca tattggcgca aaaggtaccg
      781 ctattttttc atttcatgcc attaaaaata ttacctgtgc tgaaggtggc ctgattgtaa
      841 ctgataatga aaaccttgcc cgccagctac ggatgctgaa atttcacggt ctgggtgtcg
      901 atgcctatga cagacaaacc tggggccgtg caccgcaggc tgaagtctta acaccgggct
      961 ataagtacaa tctgaccgat attaacgccg cgattgccct gacacagtta gtcaaattag
     1021 agcacctcaa cacccgtcgg cgcgaaattg cccagcaata tcagcaagca ctggcagctc
     1081 tcccctttca gccattaagc cttcccgcct ggccgcacgt tcacgcctgg catctgttta
     1141 ttattcgtgt cgatgaacaa cgttgtggta tcagtcgcga tgcgttgatg gaagcgttaa
     1201 aagaaagagg cattggtacc gggttacatt tccgcgccgc tcacacacaa aaatattatc
     1261 gcgagcgttt tcccacgctg tcgttaccga ataccgaatg gaatagcgaa cgcatctgtt
     1321 cgttgccgct gttcccggat atgactaccg ccgatgccga ccgcgtcatc acagcccttc
     1381 agcaactcgc aggacaataa gccatgtttg aaatccaccc tgttaagaaa gtctcggtgg
     1441 ttattcccgt ttataacgag caggaaagct taccggaatt aatcaggcgc accaccacag
     1501 cctgtgaatc gttggggaaa gagtatgaga tcctgctgat tgatgacggc agtagcgata
     1561 attccgcgca tatgctggtc gaagcctcac aagcggagaa cagccatatt gtgtctattt
     1621 tgcttaaccg caattacggg ca
//
LOCUS       DBKEKI010000293         1634 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.299, whole genome shotgun sequence.
ACCESSION   DBKEKI010000293 DBKEKI010000000
VERSION     DBKEKI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.299"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..1004)
                     /locus_tag="ACUQPX_004385"
                     /pseudo
     CDS             complement(1..1004)
                     /locus_tag="ACUQPX_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196353.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1202-like element ISEsa1 family transposase"
BASE COUNT          447 a          390 c          347 g          450 t
ORIGIN      
        1 cactgctttt gtactggaaa gtgagatttt tagacagaac gcgcttcgcc tgtaggctga
       61 agatataccc cagttcctct tcagaatggt gcacatcaag atgagcatta tcagtagtac
      121 gaggcgacgt agcgaaccgg ttgttatagg cttcaataaa ggtcggcaac catgcatttg
      181 ctgtttcaat atcactgata ttctgaagcc gcatttcttt gaccagcctg tcctgtagtg
      241 tctgattggc gcgctctacc cgcccttttg cctgcgggct gttggcatgg attggctcga
      301 tgcccagtgt ctttatcgca cgagtgaact gggtcagctc accttcccgc tctgggttat
      361 ttaccctgaa tatactgtgt ctatcagagt agagagcgag cggtacgcca tgatcattaa
      421 ggtaaccccg gagggtttcc atgtaagccc gggttgtttc agcaggcaca aaacgcaacg
      481 ccatcaacgc actggtggca tcatcaatga aaacgatcag tgtacatctg gggcctcgat
      541 tttcaaacca gtcatgaggt gagccatcaa tctggatcag ttcaccgtaa gatggtcgtc
      601 gcatgcggcg ttgatatata cgggcaattt tacgacggcg ttcacgccat accccctctt
      661 ctatcatcca ctttctgaga gtttcaacgg ataaagataa accgtgtatc tcgcgcaatt
      721 tttcgcacgc aaacgtaggt ccaaaatcag cgtaacggcc tttgaggagt gaaattactg
      781 ttgctctgaa ttcagtagaa aaggaattat taggacgctt tccacgtcgg tgggaaacca
      841 gaccagaagg cccttcattt ctataccgtt gcaccagacg tttaactaga aatgccaatg
      901 cgtgccgcag cttgttcctg agtaatatgt cgattaattg actcctgaat aatctgaagt
      961 cggtggagtt ccttatgact catcgtaaca gtctctttga tcatgaaaaa tcccccagag
     1021 aattgtctgg ggacatttta gaatggttca aaggggacat tacagcttgg tgttaacaat
     1081 agcctgcttc tccttgcctt tcggcacgta agaggctaac ctacatgtgt ctggcatgaa
     1141 attggcctca gattaatgtt aagcgtcttg caggacgcgt aatgttaact ggggcttttc
     1201 tctacctgcc gttggtgttc gtgcccgagg cagatagcct caagcacccg cagtcattct
     1261 acttaactaa gatttccccg caaaccgttt ttgtccagca cagtaaatat ccaactaaac
     1321 caatggagtt cgctgtattt accgccagta ttcaatgcac atgaccgcca tgaacacccc
     1381 taaaaaaagg gcatttatat atccaaatat taatatcaaa acatcaactt tttccatata
     1441 ccttgctgtg aagatgatgg gcatacatga tgcgaacaac cagaacgcaa caaacaaaaa
     1501 ctgcaatgcg tttttcatta ttcctcctac aatcaatgtg caattacatt taaacacacc
     1561 tcaatttggc cggacatata aatatctaaa ccagaaaaaa tcacttacat agcgttacaa
     1621 actctttagt ctaa
//
LOCUS       DBKEKI010000294         1618 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.300, whole genome shotgun sequence.
ACCESSION   DBKEKI010000294 DBKEKI010000000
VERSION     DBKEKI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.300"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(847..1326)
                     /locus_tag="ACUQPX_004386"
                     /pseudo
     CDS             complement(847..1326)
                     /locus_tag="ACUQPX_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248301.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
     gene            complement(1254..1618)
                     /locus_tag="ACUQPX_004387"
                     /pseudo
     CDS             complement(1254..1618)
                     /locus_tag="ACUQPX_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          364 a          451 c          384 g          419 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacataac ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctactcgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gaggggcccc aattaagttg tcaagcatgt ttactgcgca atcgcttggt
      421 gctccgtccc tgtaccccgc attggtattc tggcgctcat ggggcttcca cttgcgcttc
      481 tcccttcaca tcggggtgac aggtccacgt tccccacaag agcctgaatc gacttcacgc
      541 cgcttttatg ccggatgccg cgcaggcagt aaacatatcc cacaggaacg actccaccat
      601 tgttttgaca tcatctatat gctttcgaca catcatcagc ggttcacttt cgttcgtctc
      661 ttcgaaccat acctgatcgg ctcacgtccg accttttccc atctcgctca ccaccacatc
      721 tcttaaatgc agcagcagtg gatggtttgg tgcctgcgcc tgcacgccga caccgagggg
      781 ccttccctca tctcttgtgc cgcatggctg cacagggact cacgtcccca ggacgcgcct
      841 tcgtggcaca cagtcatcgg catagcaaca gtaagcaacg gcaggtttcc attgccagtt
      901 ttctttgaca gcagtacttc ggcctcgctg gatactgtga ttccagtacc agcgatcctt
      961 tctggctttt ccactcaggt agcgtttatt cagatactgg tcgaactcat tcagtatgat
     1021 gttcgacagc aacggcgaga tcacaccgcc ctgtggcata ctttcactga ccgcccggaa
     1081 gagaccaaca tcaacacggc gttttccaca gcagtctcat aaaacgtgca tcactgatcc
     1141 tgcggcagac ggctttcatc agcagccggt gatgtactgt gtcgaagtag ctggacaggt
     1201 caccttcaat aacccggcgc cccgtgtttc cccgcagtcg gtgagctgta atagtacgtg
     1261 atgtctcccg cccacttctg gttcgggcca ctggcgtaaa aatcctgcgt cagcagattt
     1321 tctgatacag gcaggccgtg tgcacggtaa ctgatcgggc tgaacttctg ggaggctttt
     1381 gcgctcagtt cctgacggcg cagactggct gccacggttt ttacgttaaa gtggtaacca
     1441 gagcacacag ttcatccgtc aggcgtgggg caccgtaacg ctggactggg taaaagcctt
     1501 gcggacaacg ctgtcgcagt gctgacggaa ctgctgacgt gtgtttatcc ttgtccgccg
     1561 ctgacaccac gtataccagc cgctgcgggc cacccggagt acgcggcaca ttgccttg
//
LOCUS       DBKEKI010000295         1611 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.301, whole genome shotgun sequence.
ACCESSION   DBKEKI010000295 DBKEKI010000000
VERSION     DBKEKI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.301"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1050
                     /locus_tag="ACUQPX_004388"
     CDS             <1..1050
                     /locus_tag="ACUQPX_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HHV9058808.1"
                     /translation="IVLAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQSEIYAR
                     QGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTG
                     RLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYET
                     GRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRK
                     ARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGE
                     NALRSVAIGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPS
                     NQVHELLPWNFSSVK"
     gene            1330..1608
                     /locus_tag="ACUQPX_004389"
                     /pseudo
     CDS             1330..1608
                     /locus_tag="ACUQPX_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          434 a          360 c          463 g          354 t
ORIGIN      
        1 atcgttctgg caccacttcc ccctaaaccg atcgaacgcg gttatgccag tgcagggtta
       61 cttgcacgga tcctggtcag caaatatatg gaacatatcc ctttatatcg ccagtcagaa
      121 atatacgcgc gacagggcgt ggagctgagc cgtaatacca tggtgcgctg ggtatcagaa
      181 atggcagaca aactccgtcc tctgtatata gcgctgaatg actatgttct ggaggcagga
      241 aaggtgcacg cagatgacac tccggtgaaa gtactggccc cggggaacgg aaagacgaaa
      301 acgggtcgtc tgtgggtata cgtcagggat gatcgtaatg cgggttcatc cctgccggca
      361 gccgtctggt tcgcgtattc ggcagatcgc aaaggagaac atccgcagct ccacctggca
      421 aagtatcagg gcgtactgca ggctgatgcc tatgcaggtt ataacgtact gtacgaaacg
      481 ggccgggtga aggaagccgg gtgcctggcc cacgcccgcc gaaaaatcca tgacgaggat
      541 gtgcgccgtc cgacagaaat gactcaggaa gcgctcagac ggatagcaga gttatacgac
      601 atagaagcgg agatacgtgg cagtccggca gaggaacggc ttgcagtcag aaaagccaga
      661 agcgtccagt tgatgcagtc gttgtacgac tggatacagt tgcagaggaa aacgctgtcg
      721 aaacatgcgg agatggcgaa ggcgttcgac tatatcctga atcactggaa tgcgctgaac
      781 gagttctgtc gtgacggctg ggtggaaata gacaacaaca tcggtgaaaa cgcgttacga
      841 tcggtggcga ttggaagaaa aaattatctc tttttcggct cagacaaggg aggagaaagt
      901 gcggcgatca tctacagtct gctggtcacc tgcaaacaga acgaagtgga gccggaggac
      961 tggttgcgcg aagtgatcga gaagctcaat gactggccgt cgaaccaagt gcatgaactg
     1021 ctgccctgga acttctcgtc tgtaaaataa tccttacgct acgtacttct cggggcgctt
     1081 accgctggcg gccgggttaa agacctgatg aatctcaaat gggatacact aaatattctg
     1141 gttaatgccg tcattaatgg ttgtcttgga attggtacga cgaatgcgtt aggtggaagt
     1201 tcaatcgttt taggagataa cgataccggg tttaaacaaa atggcgatgg tctgcttgat
     1261 gtttatgcga atggacagcg tgtatttcgt ttccagaatg gcagtatcca gagtaataaa
     1321 gctgtaaacg ttacaggacg ggtaacaccg tcagactacg gaaactttga tgcccgttac
     1381 cagcagcgaa atggtggcgt gcaggatgtg cggtatggtt atgaaatgta ttacaaccct
     1441 ggcagcaaca ccgtttcatg gacgtttcgc tcaccttcgg gacacgggct gtcagggata
     1501 tcgatatcgg ataccggccg taactcagcg gataacgtca acggtgtgta ttaccgaccg
     1561 ctgcaaaaac tgattaatgg cacctggtat aacgtagcga gtatttaaca a
//
LOCUS       DBKEKI010000296         1589 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.302, whole genome shotgun sequence.
ACCESSION   DBKEKI010000296 DBKEKI010000000
VERSION     DBKEKI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.302"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..217)
                     /locus_tag="ACUQPX_004390"
     CDS             complement(<1..217)
                     /locus_tag="ACUQPX_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHV9058809.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYYRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQEK"
     gene            complement(235..1383)
                     /locus_tag="ACUQPX_004391"
     CDS             complement(235..1383)
                     /locus_tag="ACUQPX_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9058810.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT          504 a          406 c          351 g          328 t
ORIGIN      
        1 gtttttcctg aacattccca gccatagaca aatccttcgc caaaattaaa ggggcatctg
       61 taaaacgagg gattatgcgc ggcaaataag aagtgcggta atagcgaaat gcaaaattat
      121 aacctgctgc acattttatt tttaaacgaa taaaatttgg aaaaaagcaa gtcagataga
      181 agaaacaaaa aagcagagtg tgaaacagta agttcacact ttgcttgaat tcatttacac
      241 gtgagcaacg ggtttcggcg tatgaccggc gttgcgaagc agcatcagca aatagataaa
      301 cgatacgctg gcgatcatga taaacaataa attatcggag aaattctgca ttagcatagc
      361 ggtaaatgac gggccaagca gacttcccac ggtatagctc aacagtaagg cttggttcat
      421 cgccaccagt tgatgatgtt caactttctc acaagcccag gccatcgcca ccggatatag
      481 cgtaaagcca gcggcaccga ggatgaataa cgctggggcc atcgccgcct ggctaagcat
      541 cgcaatactg ccgagaatga cgacaaagac ctgaacacgc aaaaccagca gtcgaccaaa
      601 cttatccgcc agacgtccaa tcggccattg tccgaggata cccgcactga ccagtaccgc
      661 catccagaaa ccaatactgg cattgctcac ccctttgtga ttgaggtaca gaggcatcag
      721 gccatataga gatcccagaa cgattcctga gataatgcag ccattcacgc caagccgcgc
      781 ctgacggagt tttagcattg ccgtaattga cgtcgaatca tggttttcag cctgctgatt
      841 caggacacgc gtaaacaaca gcggtaagat ccctgccaat gtcaaacctg taacccacgg
      901 caatacgctc atcagctcgg ttgagacttt gctgaccagt aactggccta aaaacgttcc
      961 cacgtaataa accatcatat acgcagcaag caaacgccca cggttacgtg acgtcccact
     1021 gcacatcagc gcgctctcaa caaccaccca aatcatggca cagccgacgc ccgcgacaaa
     1081 acgccacgcc aaccagctcc agaaaccaat catcaggcca aggccggcac agccagcagc
     1141 aaaaatgaag gaggacagat aatagctgcg gttaaagcca atgcgtttaa tgacataccc
     1201 tgtcagcaat gtaccgacaa ggttgccggt aaaataggat gagctgacaa cgcctacctg
     1261 ccatgtggac atgtgttcct gggcgagcca aagcggcacg agtgtattta acaccgcgat
     1321 cgccagagtc aacaaaagca ggccagacag caaaagcatg acaggccggg tatacgtgga
     1381 catgaattaa aaccgtgagg aagttcaaat ttcatgcgca tcatgccacc gccaaaaaca
     1441 ttgtcaatgg gtgaaaatac gcgcatatcg cagcggcggt attatcgatt tatattactc
     1501 attcataacg ctaatgctga agagatgatt aatctcatct gattgttttt attgatctaa
     1561 cacaaatttc atacgcttcg ttcagtcga
//
LOCUS       DBKEKI010000297         1580 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.303, whole genome shotgun sequence.
ACCESSION   DBKEKI010000297 DBKEKI010000000
VERSION     DBKEKI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.303"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            47..133
                     /locus_tag="ACUQPX_004392"
                     /pseudo
     CDS             47..133
                     /locus_tag="ACUQPX_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(143..223)
                     /locus_tag="ACUQPX_004393"
                     /pseudo
     CDS             complement(143..223)
                     /locus_tag="ACUQPX_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            434..523
                     /locus_tag="ACUQPX_004394"
                     /pseudo
     CDS             434..523
                     /locus_tag="ACUQPX_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            547..>1580
                     /locus_tag="ACUQPX_004395"
     CDS             547..>1580
                     /locus_tag="ACUQPX_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HHV9058811.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHS"
BASE COUNT          388 a          366 c          410 g          416 t
ORIGIN      
        1 tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt taagaacagg aagaactgtt
       61 ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg ctcaatgtgc tgactgaact
      121 gaaaaaacgc ggttggtcgc cctacctttt catcaagcca gtagtctgcc caccactgca
      181 tcatttctct gcgcttatcg agatactgag catggttgta aattcaacta acccagtgcg
      241 ttgacaaaaa attagcgcaa gaagacaaaa aatcaccttg cgctaatgct ctgtatcagg
      301 tcactaatac cagctaagta gttgattcat agtgactgca tatgttgtgc tttgtcgcat
      361 tttgtagtct attatttaat ctgtaacaac gataatacaa tgaattttaa ttggtttttg
      421 tgattttgtc cttcttctga catggaagga cacccgatgg atacccaacg attccaatcc
      481 caatttcatt ggcatttatc gtttaaattt tctggcgcga tagcagcgtg cttgtccctg
      541 tctcttgtgg gaacagggct tgctaaagct gatgattcgc ttccttccag taactatgcg
      601 ccgcccgccg ggggaacatt ctttttgctt gctgacagca gttttagcag cagtgaagag
      661 gcgaaagtgc gactggaagc gccggggcgt gattatcggc gctatcagat ggaagagtac
      721 ggcggcgtgg acgttcgcct gtatcgtatt cctgacccga tggcattttt gcgccagcag
      781 aaaaacctgc atcgcattgt ggtgcaaccg caatatctgg gcgacgggct gaacaatacg
      841 ctgacctggc tgtgggataa ctggtacggc aaatctcgcc gcgtgatgca gcgtactttc
      901 tcttctcagt cacggcagaa tgtgactcag gcattacccg aattacatct cggcaatgcc
      961 attattaaac cttcccgtta tgtacagaac aaccagtttt ccccgctgaa aaaatatccc
     1021 ttggtggaac agttccgtta tccactatgg caggctaaac cggtcgagcc gcagcaaggg
     1081 gtaaaactgg aaggcgcatc cagcaatttc atctcgccgc agccgggtaa tatttatatt
     1141 cctctcggcc aacaagagcc ggggctgtac ctcgtcgagg cgatggtagg tgggtatcgg
     1201 gcgacgacgg tggtgtttgt ttccgatacc gtggcgctta gcaaagtgtc aggtaacgag
     1261 cttctggtat ggaccgcggg taaaaaacag ggtgaagcga agcccggctc agagatcttg
     1321 tggactgacg gtcttggcgt gatgacccgc ggggtgaccg atgacagcgg tactttgcag
     1381 ttacaacata tatcgccaga acgttcatac attctgggta aggatgctga aggcggcgtt
     1441 tttgtctccg agaacttctt ctacgaaagc gaaatctaca acacccgctt gtatattttt
     1501 accgatcgcc cgctatatcg cgcaggcgat cgtgtcgatg ttaaagtgat gggccgcgag
     1561 ttccgcgatc cgttgcattc
//
LOCUS       DBKEKI010000298         1579 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.304, whole genome shotgun sequence.
ACCESSION   DBKEKI010000298 DBKEKI010000000
VERSION     DBKEKI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.304"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..1579
                     /gene="ehaG"
                     /locus_tag="ACUQPX_004396"
                     /pseudo
     CDS             1..1579
                     /gene="ehaG"
                     /locus_tag="ACUQPX_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
BASE COUNT          421 a          418 c          399 g          341 t
ORIGIN      
        1 ttcacggcag ctcatggcac cgagaccacc agcaaaataa ccaacgttaa agatggcgac
       61 ctgacgactg gcagcaccga tgccgttaac ggctctcagc tgaaaaccac caacgatgcc
      121 gtggcgacga ataccaccaa tatcgccact aacaccacca acatctctaa tctgactgag
      181 acggtgacta atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtcttcact
      241 gcagctcatg gcaacaatac cgccagcaaa atcaccaata tcctggacgg cacagtcact
      301 gcaaccagtt ccgatgccat taacggtagc cagctttatg acttaagcag caatatcgcc
      361 acctacttcg gcggcaatgc ttctgtgaat actgacggtg tgtttaccgg tccaacctac
      421 aaaatcggtg aaacaaatta ttataacgtc ggcgatgcac tggctgcgat taactcctca
      481 tttagcacgt ctctcggcga tgctctgctt tgggatgcca ccgcaggtaa attcagtgcc
      541 aaacacggta ctaatggtga cgcaagcgtg atcactgatg tcgcagatgg tgaaatttca
      601 gactccagtt ctgacgcagt aaacggctca caactccacg gcgtgagcag ttatgttgtt
      661 gatgcgctgg gggggtggtg ccgaagtcaa tgcagacggc accatcactg cgccgacgta
      721 caccattgct aatgctgatt acgataatgt cggtgatgcc ctgaatgcta tcgataccac
      781 tcctgacgac gctctgctct gggatgcgga cgccggtgaa aatggtgcat ttagcgccgc
      841 tcacggaaaa gataaaactg ccagtgtaat cactaacgtc gctaacggtg caatctctgc
      901 tgccagcagc gacgcgatta acggctcaca actctatacc accaataagt acatcgctga
      961 tgcgctgggt ggtgacgcag aagtcaacgc tgacggcacc atcaccgcac cgacttacac
     1021 cattgcgaac gccgagtaca acaacgtcgg tgacgccctg gatgcgcttg atgataacgc
     1081 cctgctgtgg gatgagactg ccaatggcgg tgctggagcc tacaatgcca gccatgacgg
     1141 taaagccagc atcatcacta atgtcgctaa tggcagtatt agtgaggaca gtaccgatgc
     1201 agtgaacggt tctcagttga atgcgacgaa tatgatgatt gagcagaaca cccaaattat
     1261 caatcagctc gctggtaaca ccgacgcaac ctatatcgaa gaaaatggcg ctggtatcaa
     1321 ctacgttcgt actaacgaca acgatttagc ctttaacgat gcaagcgcct ctggtgtcgg
     1381 cgctacagct gtaggttata acgctgtcgc gtctggtgcc agcagcgtag ccattggtca
     1441 gaacagcagc agcaccgttg ataccggtat tgcgctgggt agcagctccg tttccagccg
     1501 tgtgattgcc aaaggttctc gtgacactag cgtaacggaa aatggcgtgg tgattggtta
     1561 tgacaccact gacggcgaa
//
LOCUS       DBKEKI010000299         1573 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.305, whole genome shotgun sequence.
ACCESSION   DBKEKI010000299 DBKEKI010000000
VERSION     DBKEKI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.305"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..121)
                     /locus_tag="ACUQPX_004397"
     CDS             complement(<1..121)
                     /locus_tag="ACUQPX_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058812.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINS"
     gene            complement(147..1478)
                     /gene="tdcC"
                     /locus_tag="ACUQPX_004398"
     CDS             complement(147..1478)
                     /gene="tdcC"
                     /locus_tag="ACUQPX_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HHV9058813.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(1500..>1573)
                     /locus_tag="ACUQPX_004399"
     CDS             complement(1500..>1573)
                     /locus_tag="ACUQPX_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058814.1"
                     /translation="VSIISGGNIDLSRVSQITGFVDA"
BASE COUNT          487 a          383 c          379 g          324 t
ORIGIN      
        1 ccgagttaat accgtcggca atacctgaca ttaatacttc acagtcgctg gcatcgagta
       61 cggaaaactt aatcgaagac gaaccacagt taataaccaa aacaaccgga aattcattca
      121 tctcttttct catcctgagt tacggattaa aacagtttgt atacgatgtt caggatggtc
      181 agcagaccaa tcacggtaac aaacacgtta tccagacgac cacggtattt cgccagagac
      241 ggcgctttac ggatagcata catcggcaac aggcacagca gggatgcgat aatcggtgcg
      301 cccatggctt caatcaggtc gaggatgttc gggttggcgt aggcaacaac ccaggtggag
      361 cccatgatga agatcatgct gagagtattc agtttaccca gcgacacttt ggttttgtca
      421 cctttataac cgaacttcag aatcagacca ttcaagcctt ccagcgtccc cagatagtga
      481 ccgaagaaag atttgaagat agccacgagt gcgatgatgg aagccgcata ttccagtgta
      541 atcgcgaacg ttgttttcgt accggtcatg gacgcaaagt ggttagccag ataagaaagc
      601 actggaatat tctgcgcttt ggcttccgcc atgttggccg gagacagagt aaacaggcag
      661 ctaaaggcaa agaacatcac cactgcaacc atcagcatgc tggcacgaga aatgatttgg
      721 gaacatttac gttcggtgaa gtcgcgaccg aagtctttct catactcttc acgtttagaa
      781 accacaaagg aagagacgat tggcgagaag ttaaaggaga aaaccatgat ggaaatcccc
      841 agccagacag taatcaggat accgtcatga ccggttaacg acagcgaacc gaggtcaacc
      901 tggtcgataa ctgcagagtt ccagtaaggg atcagcgaca gagaaatcag caccaggctg
      961 gcgataaacg gccataccag gtagctcatc actttaacca tcagatcctt accaaaccag
     1021 atgacgaaag ccatcagcag caacaggaac agcgccacaa agccgcgatt cagcggtgca
     1081 aatccgagct ggttttccca gaacgtcata aaggtattgg taatagtaac gccataaatc
     1141 cacagcagtg ggcaaatcgc gaagaagtac aggaacgtga taaccacgcc gccagtttta
     1201 ccaaaatgct cttccaccgt ttccgtaatg ttgccggaag ggttagagcc agaaagacac
     1261 agacgcgcca gcgcccggtg gcaataaaat gcgatggggt atgccaatac caacatcaga
     1321 agaatcggga tcagtccgcc aaaacctgcg cggataggga agaacagcac cccggcgccg
     1381 attgccgtac caaacaagcc taacgtccat gtggtatctg atttacgcca ggacgattgt
     1441 tttgtctggc tggatacaat gctatctgaa gtactcatat cctatcctca acgaattaat
     1501 taagcgtcaa cgaaaccggt gatttgagag acgcgagaaa gatcgatatt gccgccggaa
     1561 ataatactga cgg
//
LOCUS       DBKEKI010000300         1549 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.306, whole genome shotgun sequence.
ACCESSION   DBKEKI010000300 DBKEKI010000000
VERSION     DBKEKI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.306"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            351..476
                     /locus_tag="ACUQPX_004400"
     CDS             351..476
                     /locus_tag="ACUQPX_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HHV9058815.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="ACUQPX_004401"
     CDS             complement(921..1397)
                     /locus_tag="ACUQPX_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9058816.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          358 a          406 c          387 g          398 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctctgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 actgggtcag ggcttcgccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 aacgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atccaggatg
     1501 cgacaacgtt gtgtcatcct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DBKEKI010000301         1547 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.307, whole genome shotgun sequence.
ACCESSION   DBKEKI010000301 DBKEKI010000000
VERSION     DBKEKI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.307"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>1547
                     /locus_tag="ACUQPX_004402"
     CDS             <1..>1547
                     /locus_tag="ACUQPX_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HHV9058817.1"
                     /translation="GSQQGYFLAGRSLKAPVIAASLMLTNLSTEQLVGLSGQAYKSGM
                     SVMGWEVTSAVTLIFLALIFLPRYLKRGIATIPDFLEERYDKTTRIIIDFCFLIATGV
                     CFLPIVLYSGALALNSLFHVGESLQISHGAAIWLLVILLGLAGILYAVIGGLRAMAVA
                     DSINGIGLVIGGLMVPVFGLIAMGKGSFMQGIEQITTVHAEKLNSVGGPTDPLPIGAA
                     FTGLILVNTFYWCTNQGIVQRTLASKSLAEGQKGALLTAVLKMLDPLVLVLPGLIAFH
                     LYQDLPKADMAYPTLVNNVLPVPLVGFFGAVLFGAVISTFNGFLNSASTLFSMGIYRR
                     IINQNAEPQQLVTVGRKFGFFIAIVSVLVAPWIANAPQGLYSWMKQLNGIYNVPLVTI
                     IIMGFFFPRIPALAAKVAMGIGIISYITINYLVKFDFHFLYVLACTFCINVVVMLVIG
                     FIKPRATPFTFKDAFAVDMKPWKNVKIASIGILFAMIGVYVGLAEFGGYGTRWLAMIS
                     YFIAAVV"
BASE COUNT          303 a          395 c          435 g          414 t
ORIGIN      
        1 ccggatcgca acaaggctat tttcttgccg gacgttcact aaaagcgccg gttattgccg
       61 cttcgttaat gttaaccaac ctttccacgg aacaactggt tggtctttcc gggcaggcct
      121 acaaaagcgg catgtcggtg atgggctggg aagtgacttc agcggtgacg ctgatcttcc
      181 tcgcgctaat ctttttaccg cgctatctga agcgcggcat tgccaccatc cccgattttc
      241 tggaggaacg ttatgataaa acgacgcgta ttatcatcga cttctgcttc ctcattgcca
      301 ccggcgtctg ctttctgccg attgttctct actccggcgc gttggcgctc aacagcctgt
      361 ttcacgtcgg ggaatcgcta cagatttctc acggtgcggc tatctggcta ctggtaattt
      421 tgcttggtct ggcgggaatt ttgtatgcgg tgatcggcgg actgcgcgca atggcagtgg
      481 cggactccat caacggtatt gggctggtta tcggcgggtt gatggtgccg gtatttggcc
      541 tgatcgcgat gggcaagggc agctttatgc agggcattga gcaaattacc accgttcacg
      601 ccgagaaatt aaactcagtc ggtggcccga ccgatccctt gccgattggc gcggcattta
      661 ccggtttaat tctggtgaac accttttact ggtgtacaaa tcagggcatc gtgcaacgca
      721 cgctggcgtc aaaaagcctg gcggaagggc aaaagggggc gctgttaacg gcggtgctga
      781 aaatgctcga cccgctggta ctggtgctgc cagggttgat tgcgtttcat ctgtatcagg
      841 atctaccgaa agccgacatg gcctacccga cgctggtcaa taacgttctg ccagtgccac
      901 tggtgggttt cttcggcgcg gtgttatttg gtgcggtgat cagtaccttc aacggctttc
      961 tgaatagcgc cagtacatta ttcagtatgg gtatttaccg tcgcatcatt aaccagaatg
     1021 ccgagccgca gcagctggtc accgtcgggc gcaaatttgg tttctttatc gctatcgttt
     1081 cggttctggt agcgccgtgg atcgccaacg cgccgcaggg gctgtatagc tggatgaaac
     1141 agctcaacgg catttacaac gtgccgctgg ttaccatcat cattatgggc tttttcttcc
     1201 cgcgcatccc ggcgctggcg gcaaaagtgg cgatggggat tggcataatc agctacatca
     1261 ccatcaacta tctggtgaag ttcgacttcc atttcctcta tgtgctggcc tgtacgttct
     1321 gcatcaacgt ggtcgtgatg ctggtgatcg gttttatcaa accgcgcgcc acgccgttca
     1381 ccttcaaaga tgcgtttgcg gtggacatga aaccgtggaa aaacgtcaag atcgcgtcaa
     1441 ttggtatcct gttcgcgatg attggcgtct atgtcgggct ggctgaattc ggcggctacg
     1501 gtacgcgctg gttagcgatg atcagttatt tcatcgctgc cgtagtg
//
LOCUS       DBKEKI010000302         1491 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.308, whole genome shotgun sequence.
ACCESSION   DBKEKI010000302 DBKEKI010000000
VERSION     DBKEKI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.308"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            257..1117
                     /gene="tesB"
                     /locus_tag="ACUQPX_004403"
     CDS             257..1117
                     /gene="tesB"
                     /locus_tag="ACUQPX_004403"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HHV9058818.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1166..>1491)
                     /locus_tag="ACUQPX_004404"
     CDS             complement(1166..>1491)
                     /locus_tag="ACUQPX_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HHV9058819.1"
                     /translation="TMLKRLLRVDDPCDVFGVHGVCGIVGCIMTGIFAASSLGGVGFA
                     EGVTMGHQLLVQLESIAITIVWSGVVAFIGYKLADLTVGLRVPEEQEREGLDVNSHGE
                     NAYNA"
BASE COUNT          357 a          407 c          369 g          358 t
ORIGIN      
        1 cgcaaccgct aaaccactgg ccatgtgcac gagtttcatt catttctcct tattatcaat
       61 gcaccagcgg gctaactttc ctcgccggaa gagtggttaa caaaatagta acgtcaacaa
      121 gtgtggcaca catcacgcat ttctgcctgt aattagcccg taattcagac tattgcaccc
      181 atcggaccag tggcaaattt gcgttatact caactcactt tggcttgctg cggcagcttt
      241 gttactggag agttatatga gtcaggcgct aaaaaattta ctgacattgt taaatctgga
      301 aaaaattgag gaaggactct ttcgcggcca gagtgaagat ttaggtttac gccaggtgtt
      361 tggcggccag gtcgtgggtc aggccttgta tgctgcaaaa gagaccgtcc cggaagagcg
      421 gctggtacat tcgtttcaca gctactttct tcgcccaggc gatagtaaga aaccgattat
      481 ttatgatgtc gaaacgctgc gtgacggtaa cagcttcagc gcccgccggg ttgctgctat
      541 tcaaaacggc aaaccgattt tttatatgac ggcctctttc caggcaccag aagcgggttt
      601 cgaacatcaa aaaacaatgc cgtccgcgcc agcgcctgat ggcctgcctt cggaaacgca
      661 aatcgcccaa tcgctggcgc acctgctgcc gccagtgctt aaagataaat tcatctgcga
      721 tcgtccgctg gaagtccgtc cggtggagtt tcataaccca ctgaaaggtc acgtcgcaga
      781 accacatcgt caggtgtgga tccgcgcaaa tggtagcgtg ccggatgacc tgcgcgttca
      841 tcagtatctg ctcggttacg cttctgatct taacttcctg ccggtagctc tacagccgca
      901 ctgcatcggt tttctcgaac cggggattca gattgccacc attgaccatt ccatgtggtt
      961 ccatcgcccg tttaatttga atgaatggct gctgtatagc gtggagagca cctcggcgtc
     1021 cagcgcacgc ggctttgtgc gcggtgagtt ttatacccaa gacggcgtgc tggttgcctc
     1081 gaccgttcag gaaggggtaa tgcgtaatca caattaaaaa aatagccgga ggtgaaaacc
     1141 gtccggctat tttttgcagt gcttgttacg cgttataggc attctcgccg tggctgttga
     1201 cgtccagccc ttctcgctct tgttcttccg gcacacgtag gccaaccgtc agatccgcca
     1261 gtttgtagcc aataaatgcc accacaccgg accagacgat cgtaatggcg atgctttcca
     1321 actgtaccag caactgatgg cccatcgtca caccttcagc gaagcccacg ccgcccagag
     1381 aactggcggc aaaaatcccg gtcatgatac agccgacaat gccacaaacg ccgtgcacac
     1441 cgaagacatc gcagggatca tccacccgca gcaagcgttt gagcatggta a
//
LOCUS       DBKEKI010000303         1479 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.309, whole genome shotgun sequence.
ACCESSION   DBKEKI010000303 DBKEKI010000000
VERSION     DBKEKI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.309"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            50..664
                     /locus_tag="ACUQPX_004405"
                     /pseudo
     CDS             50..664
                     /locus_tag="ACUQPX_004405"
                     /inference="COORDINATES: protein motif:HMM:NF015585.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
     gene            complement(949..1047)
                     /locus_tag="ACUQPX_004406"
     CDS             complement(949..1047)
                     /locus_tag="ACUQPX_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058820.1"
                     /translation="MNKLPAHLSRQNCKIASTNLSEIIPRRAAVLK"
     gene            1140..1345
                     /locus_tag="ACUQPX_004407"
                     /pseudo
     CDS             1140..1345
                     /locus_tag="ACUQPX_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
BASE COUNT          408 a          345 c          344 g          382 t
ORIGIN      
        1 gtaaaggggc cctgctatca atttgtcatt tgtattacgc gaaaattata tgaacgcaat
       61 aatatcgccc gattattact atgttcttac cgttgctggt cagtctaatg ccatggcgta
      121 tggcgaagga ctgccattac cggacaggga agatgcgcct cattccagaa ttaaacaatt
      181 agcgagattt gcgcatacgc atccaggagg cccgccatgt cactttaacg acattattcc
      241 actgactcac tgcccacacg atgttcagga tatgcagggt tatcaccatc ctctggcaac
      301 gaatcatcaa acacagtacg gcaccgttgg ccaggcactg catattgcac ggaaattact
      361 gcccttcatt cctgataatg cagggattct tatcgttccg tgttgccgtg gcggatcggc
      421 ttttaccgcg ggcagcgaag ggacatattc agaacggcac ggagccagcc atgatgcttg
      481 ccgttgggga acggatactc cgctgtacca ggatttagtc agcagaacgc gagccgcact
      541 ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg aatttgactt
      601 aatgaccagt gactacgcgt cacaccctca acactttaat catatggttg aagcctttcg
      661 tagggatcta aaacaatacc attctcagct taataatatt actgacgcgc cgtggttttg
      721 cggcgatacc acctggtact ggaaagaaaa tttcccgcat tcgtatgaag ctatttatgg
      781 caattatcaa aataatgttt tagccaatat tattttcgtc gacttccagc aacaaggtga
      841 aagaggactg acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg gatattacgg
      901 ttcagcgtac cggtcaccgg agaactggac gacggcactg cgaagcagtc atttcagcac
      961 ggcagcccgt cgggggatta tttctgacag atttgtagaa gcaattttgc agttttggcg
     1021 cgaaaggtga gcggggagtt tattcatggg ggatgtcctt gttcgttctc agagtagaca
     1081 acaaaaaaca aatgagatca aggtaatatc tcttcacttg cgggctcgaa cagcaggcaa
     1141 ttgccaatat aataatctgg cacaagggtg aaaaacgcgc accgcataaa ccgttgttat
     1201 tgcttacaga ttagccggat gccttaatag acatccgcgc cttttcgatt atggctcgga
     1261 aatctacgaa catctacaca gtttactgga acgttttggg ccacagcgtt cgcagcaccg
     1321 tcctgatatg ccgttctggc gattataagt cgacggattc tggcaaatag acaatgatga
     1381 tatgcttaat gatgttcaaa gaaattaggc cattattata ttattgcttg cattttcttt
     1441 cattcctaaa agcacccgcc attaatccca ggcgcatta
//
LOCUS       DBKEKI010000304         1462 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.310, whole genome shotgun sequence.
ACCESSION   DBKEKI010000304 DBKEKI010000000
VERSION     DBKEKI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.310"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..195
                     /locus_tag="ACUQPX_004408"
     CDS             <1..195
                     /locus_tag="ACUQPX_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HHV9058821.1"
                     /translation="MLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTDEVLALRLPENGSQLHHS"
     gene            complement(375..998)
                     /locus_tag="ACUQPX_004409"
     CDS             complement(375..998)
                     /locus_tag="ACUQPX_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HHV9058822.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQRIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE"
     gene            complement(949..>1462)
                     /locus_tag="ACUQPX_004410"
     CDS             complement(949..>1462)
                     /locus_tag="ACUQPX_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058823.1"
                     /translation="TRFEGVLSIRWTDARPETTEPRYRAKSLTFYGINGPIYHTRYCY
                     WPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIGGLIIAAYQGEADGDKVIRLV
                     RGQSYRGSRLGPVGISVPSTPTGTYIASPQFFITGCSEHSLPGSYCALSGVPDAHVSG
                     AMPGLFIRTS"
BASE COUNT          318 a          421 c          372 g          351 t
ORIGIN      
        1 atgctggaga atgagtaccc tcagagggtg gctgaccggc tgaaagcatc aggtctgagc
       61 ggtgatgcgg atgcggagag ggaagccggt gcacaggtga tgcgtgagac tgaacagcag
      121 atttaccgtc agctgactga cgaggtactg gccctgcgat tgcctgaaaa cggctcacaa
      181 ctgcaccatt cataatcaca tcgcataaac cacagaccgg actgactccg gaaaaacaga
      241 ggcccgcccc cgggcctccc cggattcatc cgtttccctg ttcagcctga cagcacgccc
      301 ccgcggccgg atgacagact ccgcttcggt aagcaaagcg gtcttctgtg attccgccag
      361 ttgcggctta ttcattactc aacgtcaaac gcccgaattg aagccaaatc atccagaccg
      421 ctcagctcct ctttcatttc acgctgacgg cgataaatct catcgttgcg atcgacctgc
      481 gcctgcacca ttgctgccgc cagttcttcc agttccggca tcgacagttt cacctgctga
      541 ttatcggcat cgctccacgc catatgtgtt tgtgctgtga cagattttgc cagcatgact
      601 accggggaca ggcggcccag tgagtcggga ccagcattcc agatacgacc gttccattca
      661 aacgtgaacg gcttcgcctc ctgttctgcg cgccatgctt caatttcctg acgtctggcc
      721 tctctggccg cttccagcat ttctggtgtc acagtgaatg gggctatctc accccatttg
      781 ccactttgca gttcctgcca gattcgctga cccgtcggtg cgacatcatc agcggtggct
      841 gtgtagggga ctgcctggtc cctgtcgtca aaaaaaacgt cacagtctac tgcgccactt
      901 tcggtataac ggggattaat gattttttta atttccacgg tgcattcctc acgatgtgcg
      961 aataaaaagc ccgggcattg cgccagagac atgtgcatcc ggcaccccgg acagggcgca
     1021 atatgacccc ggtaatgaat gctctgaaca tcccgtaatg aaaaattgtg gggatgctat
     1081 atacgttccg gtgggagtac tgggcactga aatccccacc ggtcccagtc gtgagcctct
     1141 gtatgactgc cccctgacaa gtctgatgac tttatcaccg tcagcttctc cctggtacgc
     1201 agcaataatc agcccgccaa tgtcagggtc tccccatctg ttgcggacag agctcgccac
     1261 gattctgtaa ataatatctt ctgtggttat atttattttc acccagccag tcagtctgga
     1321 tatgggccag tagcagtagc gggtgtgata aatggggccg ttaatgccgt aaaaagtaag
     1381 ggatttggct ctgtacctcg gttctgttgt ctcagggcgt gcatcagtcc accggatgct
     1441 gagcacccct tcaaaccgtg tg
//
LOCUS       DBKEKI010000305         1412 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.311, whole genome shotgun sequence.
ACCESSION   DBKEKI010000305 DBKEKI010000000
VERSION     DBKEKI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.311"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..135)
                     /locus_tag="ACUQPX_004411"
                     /pseudo
     CDS             complement(1..135)
                     /locus_tag="ACUQPX_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            510..1259
                     /gene="phoC"
                     /locus_tag="ACUQPX_004412"
     CDS             510..1259
                     /gene="phoC"
                     /locus_tag="ACUQPX_004412"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HHV9058824.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1314..>1412)
                     /locus_tag="ACUQPX_004413"
     CDS             complement(1314..>1412)
                     /locus_tag="ACUQPX_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058825.1"
                     /translation="KLPTRRERAFPRVVKERPWKYPTALKKSQSVA"
BASE COUNT          389 a          293 c          295 g          435 t
ORIGIN      
        1 tgttttcaga ccaaactgct cctgtatgac ccggtaatcg tatgcgcagt actgtgaacc
       61 tcgatcggag tggtggatta gcccggcagg tgggcgctgg ctcctgagca ccataaacag
      121 ggctttaccc gtcaatattg gctccttctg atgccattct atttcaggaa ggagtgtccg
      181 ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat atgttctact
      241 aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt ccccttgttg
      301 gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct ttttatgctt
      361 gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt tatttaaatt
      421 atgtgatagc gcacacaatt ttattatcca gtactttata ctcttaataa aataattata
      481 aatatttatc ctctataagg agatgttata tgaaaagaca gctttttact cttagtattg
      541 tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat gtgacaacaa
      601 aacctgacct ttactacctg acaaacgata atgctattga cagtctggca ttattaccgc
      661 ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat gaaaaggggc
      721 gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat ctcagtagtg
      781 gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct aaagattcac
      841 cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg gcaacgcgtt
      901 cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt tctacgtgta
      961 atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt catacatcga
     1021 ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag gataccattc
     1081 tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac tggcaaagtg
     1141 atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac tcaaatcctg
     1201 tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag aaaaaataat
     1261 gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt cagttaagca
     1321 actgactggc tctttttcag ggctgtgggg tatttccagg gcctctcctt taccactctc
     1381 gggaaggccc tttcccttct tgtcggtaat tt
//
LOCUS       DBKEKI010000306         1407 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.312, whole genome shotgun sequence.
ACCESSION   DBKEKI010000306 DBKEKI010000000
VERSION     DBKEKI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.312"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(273..>1407)
                     /gene="ospC4"
                     /locus_tag="ACUQPX_004414"
     CDS             complement(273..>1407)
                     /gene="ospC4"
                     /locus_tag="ACUQPX_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HHV9058826.1"
                     /translation="VSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSN
                     NILNIKSFDKIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTV
                     DFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGN
                     QGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQL
                     EYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKA
                     DIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVD
                     VNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          440 a          248 c          229 g          490 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt tgctttttga aattttttgt ttacatcaac caatccatta
      421 ttgataaaat attctagcac tttatagttg gcaccatctt tactaagtaa gtattcaaca
      481 tcatacagat tacaatatct attgttgttc attttctcca tctctgcgac atcttgtttt
      541 gtaaaattaa atttagatag taaataaagt gctatatcag cttttttatt gcctaaagaa
      601 taagcaaggg cctgatgagc cattttttta tcagtcactt tattgttaat ctcttcataa
      661 ttagatgctt taatcgcatc ctccaaagat atgtctctga gttttatttt tttgaagtta
      721 tctgtactta gcattcttgg tatatgatac tcaagctgaa acacaaagtt tataattcta
      781 tccagactaa caggatcaat atttttgtta taacaaaact ctctgaatcc ttgatcttta
      841 ctctttctta taaaatcaat aagatgcaac ccaattccaa gcctcatatc tttagtgtta
      901 aatattggaa catcagtttt cccctgatta ccatgcacat acctgctaac ttcatccaca
      961 acctctttaa agttatctaa gaaaaatgat tggtgctcat ggtaaaaaac aggtggaata
     1021 gcattatcaa agtgatcggt taatgtcata tatccatatg gagagtcatg atcaatgata
     1081 tacgcattgg cgccaaaatc tacagtatga tgtgttttat ttagtgggag tttttcttga
     1141 tgattggaaa tttcaacccc aaaaaaaaca aaatcgtggt tagagatttc tttaatatcc
     1201 tcagagtatg tattcttatg tgtttgtata ttttcagact gaattttatc aaaggattta
     1261 atattaagaa tattatttga cagattagag ttactttgat gtctgaagtt aaaattagaa
     1321 ttaataataa tagaaagtaa gtgacgttcg tctgaagaaa gaggatgaac tttttgtatt
     1381 atctcagaga tatagtgcct ggacacc
//
LOCUS       DBKEKI010000307         1395 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.313, whole genome shotgun sequence.
ACCESSION   DBKEKI010000307 DBKEKI010000000
VERSION     DBKEKI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.313"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            202..>1395
                     /locus_tag="ACUQPX_004415"
     CDS             202..>1395
                     /locus_tag="ACUQPX_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HHV9058827.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQGS"
BASE COUNT          291 a          435 c          377 g          292 t
ORIGIN      
        1 ggggtgttag ccagccagac ccggcatgat tactgccccc agtcgtccat gatccggggg
       61 gtgatgtcac cgggtctggt ggggcgctgg taaccgctaa taggggtcag gtcaggcact
      121 tttgccggga ccgtctgtaa cgtggatgcc ggtacctgct ccccgtggtt atctggttaa
      181 cccatataca agggagacag aatgaccgaa tccagcgatt acgaatccgt ccaggtcttt
      241 atcggcgttg atgtcggtaa agatacgcat cacgctgttg ccattaatcg ttcaggtaaa
      301 cgcctgttcg ataaagcatt acccaacgac gaaaacaaac tcaggtcgct aatatctgac
      361 ctgaaacaac atggtcagat actgctggtt gttgatcagc cagctaccat cggtgcgtta
      421 cctgtcgccg ttgcccgctc agaaggagtc cttgtcggat acctccctgg actggccatg
      481 cgccgcatag ccgacttaca cgccggtgaa gctaaaactg atgctcgtga cgctgccatc
      541 attgccgaag ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag
      601 caaatcgccg aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg
      661 caggccagca accgtatccg cggccttctg acccagatac atccggcact ggagcgcgtt
      721 ctcggtccga gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca
      781 gaaaaactcg cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct
      841 cgtctgggta aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc
      901 gttcccggca cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg
      961 ctgcgtaagc aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct
     1021 ctttacccgg tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc
     1081 accgaggtcg cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt
     1141 gcgccggtaa ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt
     1201 aataaagctc tcaaacgggc gttgttcctg tcggccttcg ccgcgctcag ggatccgctc
     1261 tccagggctt actacacccg caaaatgagt cagggaaaac gacacaatca ggcgcttatc
     1321 gccctggcga gacgacgctg cgacgttctg ttcgccatga tgcgcgacgg gactttttat
     1381 accccgcagg ggtca
//
LOCUS       DBKEKI010000308         1385 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.314, whole genome shotgun sequence.
ACCESSION   DBKEKI010000308 DBKEKI010000000
VERSION     DBKEKI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.314"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1210
                     /locus_tag="ACUQPX_004416"
     CDS             <1..1210
                     /locus_tag="ACUQPX_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HHV9058828.1"
                     /translation="AALCEKHNVELVAIGNGTASRGTERFYLDVQKQFPKVTAQKVIV
                     SEAGASVYSASELAAQEFPDLDVSLRGAVSIARRLQDPLAELVKIDPKSIGVGQYQHD
                     VSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTRVAGLTRMMAQNIVAWRDENGQFQ
                     NRQQLLKVSRLVPKAFEQCAGFLRINHGDNPLDASTVHPEAYPVVERILAATQQALKD
                     LMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEKPGRDPRPEFKTAQFADGVETMND
                     LQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISSLSNKFVEDPHTVVKAGDIVKVKV
                     LEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQNNRPAAKPRGREAQPAGNSAMMD
                     ALAAAMGKKR"
BASE COUNT          341 a          378 c          383 g          283 t
ORIGIN      
        1 tgctgccttg tgtgaaaaac ataacgttga actggtggcg atcggcaacg gtacagcttc
       61 ccgcggaact gaacgtttct atctcgacgt gcagaagcag ttcccgaaag tgaccgcaca
      121 gaaagtgatc gtcagcgaag ctggcgcgtc ggtttactca gcttccgagc tggcagcgca
      181 ggagttcccg gatctcgacg tttcgctgcg tggtgcggta tctatcgccc gccgtttgca
      241 ggatccgctg gcggagctgg tgaaaatcga tccgaaatct atcggcgtag gtcagtatca
      301 gcatgacgtc agccagacgc aactggcccg caaactggat gcagtagtag aagactgcgt
      361 aaacgccgtt ggcgtcgatc tcaacaccgc ttctgttccg ctattaaccc gcgtggcggg
      421 cctgacgcgc atgatggcgc aaaacatcgt ggcctggcgc gatgagaacg gccagttcca
      481 gaaccgtcag caactgctga aagtcagccg tctggtgccg aaagccttcg agcagtgcgc
      541 gggcttcctg cgcattaacc acggtgataa cccgctggac gcctctaccg ttcatccgga
      601 agcctatccg gtggtggaac gcattctggc agcaacacag caggcactga aagatctgat
      661 gggtaacagc agcgaactgc gtaacctgaa agcgtctgac tttactgatg aaaaattcgg
      721 tgtgccgaca gtaactgaca tcatcaaaga gctggaaaaa ccgggtcgcg atccgcgtcc
      781 ggaatttaaa accgctcagt ttgccgatgg cgtcgagaca atgaacgacc tgcaaccggg
      841 tatgatcctc gaaggcgcag tgaccaacgt caccaacttt ggcgcgtttg tcgatattgg
      901 cgtgcatcag gacggcctgg ttcacatctc ttcattgtcg aacaagtttg tggaagatcc
      961 gcataccgtg gtgaaagcgg gcgacattgt gaaggtgaaa gtgctggaag tggatcttca
     1021 gcgtaaacgt atcgccctga ctatgcgtct ggacgagcag cctggcgaaa ccaactctcg
     1081 tcgcggcggc ggtaatgaac gcccgcaaaa caaccgcccg gcagccaaac cacgcggtcg
     1141 tgaagcgcag cctgccggta atagcgcgat gatggatgcg ctggcggcag cgatgggtaa
     1201 aaaacgttaa acgcccggac tggcccctaa acccgcgcga taaggcgttc acgccgcatc
     1261 tggcaacgaa cgctttgcct gacataaaag tgccggagaa tatctccggc acttttattc
     1321 cacagccaaa ctcacaatat actccgttaa tatttatcac ttcattaaca actgaaacct
     1381 taatt
//
LOCUS       DBKEKI010000309         1362 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.315, whole genome shotgun sequence.
ACCESSION   DBKEKI010000309 DBKEKI010000000
VERSION     DBKEKI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.315"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..269
                     /locus_tag="ACUQPX_004417"
     CDS             <1..269
                     /locus_tag="ACUQPX_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HHV9058829.1"
                     /translation="PSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTD
                     PEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            271..875
                     /locus_tag="ACUQPX_004418"
                     /pseudo
     CDS             271..875
                     /locus_tag="ACUQPX_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            862..>1362
                     /locus_tag="ACUQPX_004419"
     CDS             862..>1362
                     /locus_tag="ACUQPX_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHV9058830.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKE"
BASE COUNT          325 a          319 c          379 g          339 t
ORIGIN      
        1 tgccttcaga tattacccga caggtacagc aatccgtgct ggacgcattt aatggtacag
       61 atggtggtct gcgggcaagg gttgcctctg ttgtctctgc cgggcgctac tatgccggca
      121 tttacaaaac cgatccggaa catattgata ttctgggcct tactgtgagc cgtgacggtt
      181 cgtcatggac aacggctgtc actttcggga tagatgagat tccggttctg gatgtgtcgg
      241 atatcagtgt gaaactccag gaggcataac gtgcagaatg tggctgccac tgtgcttgcg
      301 cagtatgctg ccagcccccg actcaatgcc ctcattaaca gctttaacgc agcgctttcc
      361 cccgacagtt ttatcagtga tttttatggt cttatctgga acatcgatac cgcagaaaag
      421 tatggtcttg atgtctgggg aaagattgtg ggtgtcagtc gccggctgac ggtaaaggac
      481 gattttaatt acctggcttc agcgagtcca ggatggacac cccggtaatg gatgatccct
      541 gtccgtttaa tcaggcaccg ttttacaacg gaaaatcgga tacccggact gttgacctgt
      601 ctgatgctgt ataccggcgg ctgatactga tgaaagccat gtcgaacatt actgactgtt
      661 ccgttccgga tattaaccgg atgctgagat ttatgttcgg aaaaaaacgg cgggcttatg
      721 ttctgaataa tggtgggctg aggatgagtt acatctttga gtccgcgctc tcgttggcag
      781 aactggcgat tatccagttg tcgggtgcac tgccatcccc gccgggtgtt tatgtttcag
      841 tagttttaaa ggagtcccgt aatgaaggcc agtgataaac cccgccagct ggcggtcccc
      901 tttgcgagta ccggagataa aaaccgtatc ccggacaagg cgacacagca gaccagagag
      961 agcggtaatg ctgcgtatga ttcaggtttt cctccggtga ccatgacagc ggtctcagcg
     1021 ggaggtatac cgccacacgg caaggatttt aacggtctga tgtacgatat taccgcagca
     1081 atacggttcg cccaggctgg cggtttgtac acgtataatg ccggttttgc gggggccatt
     1141 ggtggatatg caaaaggagc cattctcgcc ggagtcgcaa caacagcggt ctggctgaat
     1201 accacagacg ataacctgac cgatcctgaa ggctccgaca gtgcgggctg ggtaaatctt
     1261 cttgaggatc cgaaaaggat attcctgcgg cagaagaaca atctgtcaga ccttcagaat
     1321 aaagggacgg cacgggataa tcttcaggtt tacagtaaag ag
//
LOCUS       DBKEKI010000310         1349 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.316, whole genome shotgun sequence.
ACCESSION   DBKEKI010000310 DBKEKI010000000
VERSION     DBKEKI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.316"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..502)
                     /locus_tag="ACUQPX_004420"
     CDS             complement(<1..502)
                     /locus_tag="ACUQPX_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp138 family membrane-puncturing spike protein"
                     /protein_id="HHV9058831.1"
                     /translation="MAQPLSNPTDVNSEINAQDFMLRQFLGRHAFITLGRVVAVEEGF
                     IEARPMVMGVAADGSPVEHEVIYNIPVWRLQGGGNAVIMPPHVGDIGFLAICDRDISA
                     VKATRQAAMPGSKRTHNYADAIWLGGVLNGDPVQFVVFDDNQIRIVSPWKVEISAPEG
                     VINAPKS"
     gene            complement(483..>1349)
                     /locus_tag="ACUQPX_004421"
     CDS             complement(483..>1349)
                     /locus_tag="ACUQPX_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate hub protein"
                     /protein_id="HHV9058832.1"
                     /translation="SYRQRKITVEFTLSDGRTFGNGQGNMLTITDASCFASIAVYGGV
                     AGTQITLYIWGMSPAHMTNLSWRGVWRQEQSTANKMRLWADGRLIFEGDITDAYADYN
                     QAPDIPLILTGQIHFNLRNQPAADFSAKGDVAVADIIRALASTVGLGFENQGVSRSLS
                     DPHFSGNVVQQMLDVASAADINIDLGNVEKVTIWPKGQNRNIPPVLISPDHGLTGYPV
                     YTMTGLSATTIFCPDLFTGRPAHLEPSLPDMTGDYTITGVIHTITSRTVGGPWSSNCT
                     MMRAEENGTTTQ"
BASE COUNT          318 a          393 c          317 g          321 t
ORIGIN      
        1 agcttttcgg cgcattaatc acaccctcag gcgcggatat ctccactttc cagggagata
       61 caatccgtat ctggttgtca tcaaatacca caaactggac gggatcaccg ttgagcacgc
      121 cacccagcca gatggcatca gcataattat gggtacgctt tgatcccggc atcgcagcct
      181 gacgcgtggc ttttaccgca ctgatatccc gatcgcagat ggcaagaaaa ccgatatccc
      241 ccacatgtgg cggcataatc accgcattac cgcccccctg tagtcgccat acgggaatgt
      301 tataaatcac ctcatgttca accggggaac cgtctgctgc aacgcccatt accatcggtc
      361 gggcctcaat aaatccctct tccacggcta ccacccgtcc cagagtgata aacgcatgac
      421 gtccgagaaa ctgccgcagc ataaagtcct gtgcattgat ttcgctgttc acgtctgtcg
      481 ggttactgag tggttgtgcc attttcttca gccctcatca tggtacagtt ggaactccac
      541 ggaccgccca cggttcgcga agtaatggtg tgtatcaccc ctgtgatcgt ataatcgccc
      601 gtcatatcag gcagtgacgg ttccagatgc gctggcctgc cagtaaagag atcggggcag
      661 aatatcgtgg tggcgctgag tccggtcatg gtgtaaaccg gatagccagt cagtccgtga
      721 tccggcgaaa tcagtaccgg cggaatattc cggttctgcc ctttcggcca gatggtgact
      781 ttctccacat tccccagatc aatgttaata tcggcggctg aagcgacatc cagcatttgt
      841 tgtacaacat taccggaaaa atgcgggtca gacagactgc ggctgacccc ctggttttca
      901 aatccaagcc cgacagttga cgccagcgca cggataatat ctgcaaccgc aacatcgccc
      961 tttgcgctga aatctgccgc cggctgatta cgcagattaa aatgaatctg tcctgtcaga
     1021 atgagcggaa tatccggcgc ctggttgtag tcggcgtatg catcagtaat atcaccttca
     1081 aaaataagcc gcccgtcggc ccacagccgc attttatttg cggtactctg ctcctgtcgc
     1141 cacacaccgc gccagcttaa attcgtcata tgcgccggtg acattcccca gatatacagc
     1201 gtgatttgtg taccagccac ccctccatat acggcaatac tggcaaaaca gcttgcgtcg
     1261 gtaatggtca gcatattgcc ctggccatta ccaaacgttc gtccgtctga aagagtgaac
     1321 tccaccgtta ttttacgttg tctgtagct
//
LOCUS       DBKEKI010000311         1301 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.317, whole genome shotgun sequence.
ACCESSION   DBKEKI010000311 DBKEKI010000000
VERSION     DBKEKI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.317"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(98..289)
                     /locus_tag="ACUQPX_004422"
     CDS             complement(98..289)
                     /locus_tag="ACUQPX_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HHV9058833.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            354..677
                     /locus_tag="ACUQPX_004423"
     CDS             354..677
                     /locus_tag="ACUQPX_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HHV9058834.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQR
                     VQDDS"
     gene            667..>1301
                     /locus_tag="ACUQPX_004424"
     CDS             667..>1301
                     /locus_tag="ACUQPX_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HHV9058835.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAENELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGR
                     LELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLP
                     DNLPETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGLEVVRVTRSS"
BASE COUNT          299 a          315 c          419 g          268 t
ORIGIN      
        1 gacacccgtt aaaacctgct gacgcgccct gacgggcttg tctgctcccg gcatccgctc
       61 acagacaagc tgtgaccgtc tccgggagct gcatatgtca gaggttttca ccgtcatccc
      121 cgaaacgtgc gaggcagctg cggtaaagct catcggcgtg gtcgtgaagc gattcacaga
      181 tatcagcctg ttcatccgcg tccagttcgt tgagcttctc cagcagcgtt aatgtctggc
      241 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttagtcact gatgcctccg
      301 tgtaaggggg atttctgttt tatggggtaa tgataccgat gaatcgcgag aggatgctca
      361 ccatacgggt tactgatgat gaacatgccc ggttactgga acgctgtgag ggtaaacagc
      421 tggcggtatg gatgcggcgg gtctgcctgg gtgagccggt tgcacgctcc gggaagctgc
      481 cgacactggc accgccgtta ctgcgtcagc tggccgccat tgggaataac ctgaatcaga
      541 cagcgaggaa ggtgaacagc gggcagtggt cttccggtga ccgggttcag gtggtggccg
      601 cactgatggc catcgagaga gagttgcgca gcttacggca ggtggtcagg gaacagagag
      661 tgcaggatga tagttaagtt tcatgccagg ggaaaaggtg gtggcagtgg tccggttgat
      721 tacctgctgg gaagggagcg aaaccgggaa ggggcaacgg tgcttcaggg taatccggaa
      781 gaagtacggg aactcatcga tgccacgcca tttgcgaaga aatacacgtc cggtgttctg
      841 tcgttcgcgg agaatgagct gccgccggga ggacgtgaaa aagtgatggc gagctttgag
      901 cgtgtactga tgcccggtct cgaaaaaaat cagtacagca tcctgtgggt ggagcaccag
      961 gacaagggac ggcttgagct gaattttgtc attccgaaca tggagctaca gaccggaaaa
     1021 cgcctccagc cgtactacga ccgcgcagac aggcctagaa ttgatgcctg gcagacgctg
     1081 gttaatcacc attacgggct gcatgacccg aacgccccgg agaaccgcag gacgctgaca
     1141 ctccctgata atctgccgga aacgaaacag gcgcttgcag agagcgtcac acgcgggata
     1201 gacgctcttt atcatgtcgg ggagataaaa ggccgtcagg atgtgattca ggcgctcaca
     1261 gaggcagggc ttgaagtggt cagggtgacg cgaagcagta t
//
LOCUS       DBKEKI010000312         1299 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.318, whole genome shotgun sequence.
ACCESSION   DBKEKI010000312 DBKEKI010000000
VERSION     DBKEKI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.318"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..715)
                     /locus_tag="ACUQPX_004425"
     CDS             complement(<1..715)
                     /locus_tag="ACUQPX_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HHV9058836.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTN"
     gene            1207..>1299
                     /locus_tag="ACUQPX_004426"
     CDS             1207..>1299
                     /locus_tag="ACUQPX_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HHV9058837.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIV"
BASE COUNT          381 a          209 c          261 g          448 t
ORIGIN      
        1 gatttgtcgg gattgtagtt atttggttgt tttcaaaata aaaaaatctt ctcatgataa
       61 tattttcttg gatgtttggt aagttgctta atctattgct atatgcaaaa agaagttgca
      121 gtctcctagg taaagggggc agttcagtaa gatggttgtg cgcaacctta agtatcttaa
      181 gattagaagg cagagatgga agagtttgca gttcgttgtt cctaacctca agttttttta
      241 aattcaaggg taatgaaggc agtgtggata acctattata ctctgcgctt agtgatttga
      301 tggtgtctgg taataacggc agattctcta attgattttc ggacacatcc aaagttatta
      361 gataatgtgg taatgcagat atatgtgtta agaaattatc tcttgcaata agttctttaa
      421 tattttcagg taaatcagta aggatggtta atttattaca gctcacatca agctttttaa
      481 tgcaagcagg gagtacaggt aacgaggaaa gcgccaaggc tcgcaggtcc agtttatgcc
      541 ccttagaggt aagacatgca cgaagtcttc ttaccgcctc agtacgctgt tcttccggta
      601 acccctgttt ctcccaatca tcccattcgg acaaataatg gttgggggat gtaattgtgc
      661 tgatatgtgg aaataaacag gatgaaatat ttctgagcat attaatttct cgcatatgga
      721 tatggaatta gattgtgaat aaattgtatt taatgtatct ctcctaatga ggagggtgtt
      781 tcagaatatt cgccataagg gaatgagtgt tatatcatgc tttctaccct ggtaagtata
      841 tttgcttttt tcgtatattt ggttcatctt tttttgatgt tttacatatt cacaatgcag
      901 aggatttata ccagaggaca ttagtcttga gtaagatact acaacatctc caactatttt
      961 agccagcctg actacttgac ttggtaatat ttgttgctaa taatcatgta taaaataagc
     1021 ttatatacat agtaacttca gcaaaggatt tagtaatgat ccaaatcata tctcaatgta
     1081 ctgttttttt ctttaaaaaa acaatacatt ctattttata atgactgtgt attcccatgg
     1141 aaggagatca cccaattctc cccccccgac ttttgacgca gtgtcttgtt aacaggggga
     1201 tttaccatgc ttattggcta tgtacgcgta tcaacaaatg accagaatac cgatctacaa
     1261 cgtaatgcgt ttatttgtgc cggaagtgag ttgattgtt
//
LOCUS       DBKEKI010000313         1296 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.319, whole genome shotgun sequence.
ACCESSION   DBKEKI010000313 DBKEKI010000000
VERSION     DBKEKI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.319"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..1296
                     /locus_tag="ACUQPX_004427"
                     /pseudo
     CDS             1..1296
                     /locus_tag="ACUQPX_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          393 a          264 c          308 g          331 t
ORIGIN      
        1 taacaacggg taatggaagt tacgggtatc cgttgcagga gagggtagca agttaaccat
       61 taacaatggc gtcattgtaa caacgggcgg tttatatcct tctgacaata gtcctctaag
      121 cccagcatta acagctagcg caatcgtaag tgaatttggc ggtgctgtta ttttgcaggg
      181 taaaaacacg attgtcacaa cgggggatta ctcaattggt ttgctaagtc aaacgagtgg
      241 aaacctgaat actgacacca taataagagt caactctgac ggttcggtta ccccctcttt
      301 ttctgacgga gatgatacat ttattgttac tgcgggtaat cacgcagtcg gcgttcttgc
      361 gtgtgcatct cccggaagcg cgcgtgcgtg tgtatcttct cttgatgaag aaagtactgc
      421 cgatacagga agtaatgaaa ataatgccat agcaaaactg gatatggcaa aaggggagat
      481 cacaacccac ggaacagaaa gttatgccgc gtacgctaat ggcactgtcg tcaaggctgg
      541 tgacacgctt gattatacca atgccagcgt tacattaacc gatgttgata tcaccacgca
      601 tggggacaat gctcatgcca tcgctgcccg ccaggggaca gtttcattta accagggaga
      661 aatttataca acgggtcctg acgccgcgat tgctaaaatt tataatggcg gcacggtgac
      721 gctgaaaaat acatctgcag tcgcgcatca ggggtctgga attgtgctgg agtcttccat
      781 aaatggtcag gaagcaacgg tagatatttt atctggtagc tcactacggt cagcaaatga
      841 aatcctctac cataaaaatg agacgagtaa cgtgaccatt acggatagtg aggtatcatc
      901 ggctgcagat gtttttatca ataatattaa aggtcatttg accgtcgatg caactaattc
      961 aaaaataacg ggttcagcta atatttcaac agatgataac actcatacct atctgtcgct
     1021 ttcagataat agtacctggg atattaaagc tgactcaacg gtgagcaacc tcacggttga
     1081 taatagtacg gtctatattt cccgggcaga tggacgggat gtcgaaccaa caagattaac
     1141 aataactgaa aattacgttg gtaataatgg cgtattgcat ctcagaactg aattggatga
     1201 tgataattca gctacggata aagtcgttat taatggaaat acctctggaa caacccgagt
     1261 taaagttacc aatgcaggag gcagcggagc ttacac
//
LOCUS       DBKEKI010000314         1292 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.320, whole genome shotgun sequence.
ACCESSION   DBKEKI010000314 DBKEKI010000000
VERSION     DBKEKI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.320"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..974
                     /locus_tag="ACUQPX_004428"
                     /pseudo
     CDS             1..974
                     /locus_tag="ACUQPX_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
BASE COUNT          451 a          263 c          209 g          369 t
ORIGIN      
        1 atccgactta ttaaaaatgc ctgtttaaaa ttatataatt tggcacatac cgcaaccaac
       61 tcccacctta aaccaactgg tccggataat tcagatgttc tcctgttcaa aaagctattc
      121 tccccttcga aattaatgac aattatcggt aatgaaattc ctcttatatc tgaaaaacag
      181 tcgctttcaa aggtgctttt aaatgatgag aataatgaac tgagtgatgg tacaaacttc
      241 tgggataaaa atcgtcaatt aaccacagat gaaatagctt gctatcttca gaagatcgcc
      301 gctaatgcaa aaaatactca agtcaattat cctactggtc tctacgtccc ctactccacc
      361 agaactcacc tggaagacgc tctcaatgaa aatattaaga gcgatccatc atggccgaat
      421 gaagtccagt tattccccat aaatactggc ggacactgga tattagtttc gctacagaaa
      481 acagtaaata aaaaaataat aaactacaaa taaaatgcgt catattcaac tcattgcgtg
      541 cactaggcca tgataaagaa aattcactta agcgtgtcat taatagtttt aattctgaac
      601 tcatgggaga aatgtcgaat aataatataa aagttcattt aaatgaatca gagataatat
      661 ttttacatgc cgatcttcag caatacttaa gccaaagttg cggtgcgttt gtgtgcatgg
      721 cagcccagga agtgattgaa caaagggaaa gcaattctga cagcgccccc tatacgttat
      781 taaaaaacta tgctgacaga tttaaaaaat attcagcaga agagcagtac gaaattgatt
      841 ttcaacatcg actggtaaac agaaattgtt atttagataa atatggcgat gcacgaatca
      901 atgcttctta tacacaatta gaaataaaac actcacaacc caaaaataga gcatccggca
      961 aaagagtgag ttaacatcat tgttaatcaa ttcgaccagt cagtaaatat gctgactggt
     1021 cgaacataat ttgctaaaaa gtcatcactg actgatgtca gtcccaccat ctgccagttc
     1081 aatctacggg tatttccagc gtatcgcggc acgcgattgc tggtctgttg atgtagtgga
     1141 ttgtagttgc tgacttttct taccctcaga tttctgcccc acttgtcaat tcaatctgcc
     1201 aataaataac ataattatat ttttattcct cacagaaatt cctttttaaa aatagcgaac
     1261 ccactgtaaa aaacactttt tgtatacctc ta
//
LOCUS       DBKEKI010000315         1289 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.321, whole genome shotgun sequence.
ACCESSION   DBKEKI010000315 DBKEKI010000000
VERSION     DBKEKI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.321"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            215..>1289
                     /gene="rstB"
                     /locus_tag="ACUQPX_004429"
     CDS             215..>1289
                     /gene="rstB"
                     /locus_tag="ACUQPX_004429"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HHV9058838.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDG"
BASE COUNT          326 a          306 c          313 g          344 t
ORIGIN      
        1 gagactaaag aacaacaaag cgtcacgtta aattaaacct tagcataata aacaagcccc
       61 ggtggaaaat tgaatttact gaatatgaat acgccgggga tacatccgtc tgttccggac
      121 attatcttcc aaacaaggtc ttatccagtt ggcggtttac attgctccac agtcgtgaat
      181 taatacgaat agtattctgt tgcgggcgat gccaatgaaa aaattgttta tccagtttta
      241 cctgttattg tttgtctgct tccttgtgat gtctctgctg gttgggctgg tgtacaaatt
      301 taccgccgaa cgcgcgggca aacagtcgct ggatgatttg atgaacagtt cgctgtatct
      361 gatgcgcagc gaattgcgag agatcccccc acacgactgg ggtaagacgc tgaaagagat
      421 ggatttaaat ctctctttcg atctgcgtgt cgagccactg agtaaatacc atcttgatga
      481 tatttccatg caccgactgc gtggcggcga aattgtcgcc ctggacgatc agtacacgtt
      541 tttgcagcgt atcccgcgca gccactacgt gctggcagtt ggtcctgttc cttatcttta
      601 ttacctccat caaatgcgat tgctggatat cgccctgatc gcttttattg ctatttccct
      661 cgcctttccg gtgtttatct ggatgcgtcc gcactggcag gatatgttaa aactggaagc
      721 agcggcgcaa cgatttggcg atgggcatct caatgaacgt atccactttg atgagggttc
      781 gagctttgaa cgacttggta tcgcatttaa ccagatggcg gacaatatca acgccttaat
      841 tgccagcaaa aaacagctta ttgacggtat cgctcacgaa ctgcgaacac cgttagtgcg
      901 cctgcgttat cgactggaga tgagcgataa cctgagcgcc gccgaatccc aggcgttgaa
      961 tcgtgatatc agtcaacttg aagctttaat tgaagagctg ctgacttatg cccgactcga
     1021 tcgcccacaa aacgagcttc atcttagcga accagacctg ccgttgtggc tgtcaacgca
     1081 tctggcagat attcaggcag taacgcccga taaaacggta cggataaaaa cgctcatgca
     1141 aggccattat gcggcgttgg atatgcgctt aatggagcgc gtgctggata atttgctcaa
     1201 taacgccctg cgctactgcc attcaacggt tgaaaccagc ctgctactgt cggggaatag
     1261 agcgacatta attgttgagg atgatggcc
//
LOCUS       DBKEKI010000316         1288 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.322, whole genome shotgun sequence.
ACCESSION   DBKEKI010000316 DBKEKI010000000
VERSION     DBKEKI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.322"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..735
                     /gene="icd"
                     /locus_tag="ACUQPX_004430"
     CDS             <1..735
                     /gene="icd"
                     /locus_tag="ACUQPX_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HHV9058839.1"
                     /translation="EKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEY
                     AIANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKE
                     IVIKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDEC
                     ALFEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVT
                     YDFERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(838..1161)
                     /locus_tag="ACUQPX_004431"
     CDS             complement(838..1161)
                     /locus_tag="ACUQPX_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HHV9058840.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDIKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
BASE COUNT          373 a          259 c          285 g          371 t
ORIGIN      
        1 gagaaagtga ttaaattcct gcgtgaagag atgggcgtga agaaaattcg cttcccggaa
       61 cattgcggta tcggtattaa gccgtgttct gaagaaggca ccaaacgtct ggttcgtgca
      121 gcgatcgaat acgcaattgc taacgatcgt gactctgtga ccctggtgca caaaggcaac
      181 atcatgaagt tcaccgaagg cgcgtttaaa gactggggct accagctggc gcgtgaagaa
      241 tttggcggtg aactgatcga cggcggcccg tggctgaaag ttaaaaaccc gaataccggc
      301 aaagagatcg tcattaaaga cgtgattgct gatgcattcc tgcaacagat cctgctgcgt
      361 ccggctgaat atgatgttat cgcctgtatg aacctgaacg gtgactacat ttctgacgct
      421 ctggcagcgc aggttggcgg tatcggtatc gcccctggag caaacatcgg tgacgaatgc
      481 gccctgtttg aagccaccca cggtactgcg ccgaaatacg ccggtcagga caaagtaaac
      541 cctggctcta ttattctctc cgcagagatg atgttacgcc atatgggctg gactgaagcc
      601 gcagacctga ttgttaaagg tatggaaggc gcgatcaacg ccaagaccgt aacctatgac
      661 ttcgagcgtc tgatggaagg cgctaaactg ctgaaatgtt cagagtttgg tgatgcgatc
      721 atcaagaata tgtaatcact acatgtgtta aatatttaaa cgggcgtata acacgcccgt
      781 tgttttattt atgtggatat tattaatagc atatcgagca tatttatata aaacctatta
      841 cttgagccca tatgggcata tttttataat acaactctta tgcaaacatt tatttgttat
      901 ttcgctttct cctggaggac actcttgact gcttttgagt aaactccata aattcttgtt
      961 gaatggtgca atgttataaa tagtaatagg atattctttt tccttaagga taataccaag
     1021 cttaaccggt gtaaatatcc ttccaggagg aagaaatata gtagattgat accagatgat
     1081 catttttata tcaccccata tggctgaaaa agatatgccg catgtaggtt gaattaccgt
     1141 gtcaattact acccacttca tttgttatgt cttatcccac ggtatttaat atagttcatt
     1201 tggatgttca tttctttatt ttgcatatga gtatattacc ccttcaaaaa ataaattaat
     1261 taaaacgatt gcttatataa aacaaaat
//
LOCUS       DBKEKI010000317         1268 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.323, whole genome shotgun sequence.
ACCESSION   DBKEKI010000317 DBKEKI010000000
VERSION     DBKEKI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.323"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..780)
                     /locus_tag="ACUQPX_004432"
     CDS             complement(<1..780)
                     /locus_tag="ACUQPX_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HHV9058841.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQ"
     gene            complement(1045..>1268)
                     /locus_tag="ACUQPX_004433"
     CDS             complement(1045..>1268)
                     /locus_tag="ACUQPX_004433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058842.1"
                     /translation="PPPPIMIGQYQYSTSKDRKLAVSAKHRPDGEKTKRYAKMPRFQK
                     NKKPLMDSLLNTVFYIHDSLIPVALIAII"
BASE COUNT          341 a          194 c          303 g          430 t
ORIGIN      
        1 ttgcagagca tgggatgaca atgggttatc actaatatct attgaacatt cattcctcag
       61 attaagaatg ctttccggga tatgacttat ctgatttatg tcaaaataac cgttccttaa
      121 attttccggc aggtatggaa tatcggttaa catattgtcg attgcccata tggagtgaat
      181 gttgtgaggt agagggggga gttcactaag aagattccct attgcgctaa tctcttgtaa
      241 agaaaatggt aaaggtggta aaactgccag cccattacat gatacatcta atgtttccag
      301 tagttctggt aaaacaggaa gagaacataa ttgattattt gagacatgaa gctccttcaa
      361 agatatgggg agtgtgggta gtgtgattag ttgattgtgg gacgcattca ataatttaag
      421 tccttgaggc aaagcaggca gttcaataag tctgttatag ctgacattaa gctgtgtaag
      481 ggacgcaggc aatggggaga ttaagcttaa attattttta cttatattaa ttgatttaat
      541 tcccgggggg atttcaggta atgttgtcag gcctaattca gacaagtcca ggttcgtctc
      601 ttggttttgt agacatgata ctagtcgctg aaaagcgatg tctcgttgtt cttcttgtat
      661 gcggttattt ttccattcag tccaatgggt tagataattt tcatatgcgc ggctagtgtc
      721 gattgaataa gtggagaacg aatttgaaat taatctgtga ttgttatttg tcgggagcat
      781 aaatatcagg gcgtattctt tatgttgaga ggtgttggat tcttttttat ctgctttgca
      841 atgttattgg ttcccttcag cagtagacag gaacttctgg aggaggcgga tatatagttt
      901 gtgtaagcat attaactcca tatgttatat attgaagaac ttctgcttta cactatccta
      961 aactggatcg gtttaatgta aaaaaacgat actttttggg ggagggggca atttttcctt
     1021 aaggtatgaa aaaaggagct gaagctatat aatagctatt aatgccactg gtatcagtga
     1081 atcatgtata taaaaaactg tgttaaggag gctgtccatt agcggttttt tattcttctg
     1141 gaaccgcggc atctttgcgt atctttttgt cttctcaccg tctggtcggt gttttgctga
     1201 gactgctaat ttcctgtctt tgctagtgct gtactggtac tgcccaatca tgattggcgg
     1261 gggcgggc
//
LOCUS       DBKEKI010000318         1266 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.324, whole genome shotgun sequence.
ACCESSION   DBKEKI010000318 DBKEKI010000000
VERSION     DBKEKI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.324"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..432)
                     /locus_tag="ACUQPX_004434"
     CDS             complement(<1..432)
                     /locus_tag="ACUQPX_004434"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HHV9058843.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAG"
     gene            complement(603..>1266)
                     /locus_tag="ACUQPX_004435"
     CDS             complement(603..>1266)
                     /locus_tag="ACUQPX_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HHV9058844.1"
                     /translation="LSHRKNNPKADSDAYETSADMILSELISHGEVDDQMLLNAAALI
                     RLEDWDFLESALVSWDNLPAVVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVY
                     YALDPDALAQLDVLAKQPDRIWWSTLAKSNLTFFKFGALSNRHTPPAALAAEIDPEWW
                     IVAMNNPRFPVDVLKARLKRDPLLALELVNPELDLVRQLALNGKTRAIREQAMKKLDE
                     LY"
BASE COUNT          365 a          307 c          276 g          318 t
ORIGIN      
        1 accggcataa ctgcgaacac cgttaatcgt ctgcgcgagt aaaccgcaaa actcggtcaa
       61 tatggtgcaa aataacatga gtatcacgag tgatgcgtta ctttccggta aaaataaaaa
      121 cggtaaatag agcgcaatat cggaaataac atcgccagtc tcattcaaaa tcgcccccag
      181 acgtgtttgc tgattgcact cacgcgccaa catgccatcc agcgcattga gcgccatacg
      241 gataaaaagc acgatgggca atagcaaaaa gaggatgggt tgtgccacca acaccaacag
      301 caatccggta aaaagagaaa gcgccagtgc cgtaagagtg atgtgattcg ctgtaacgtg
      361 gtgcttataa agccaaaaca tcgtcggcct taacagcgac tgaaagagcg gttttatctg
      421 gtagagcgtc attatcaatc cttgataatt cggtttgcgc tatggtgcat caattcccct
      481 gacggagata tcgcaacacg ccgcttcatt aaaaattcag aatatatcaa ggtattgggg
      541 gctcactgtc aggaatgatt atccccaata aatcaagtag ttaaaaatta ttacgctgtt
      601 attcaataca actcatcaag tttcttcatc gcttgttccc gtattgcgcg cgtcttaccg
      661 ttaagcgcca gctggcggac taaatccagt tcagggttaa caagctctaa cgccagcaaa
      721 ggatcacgtt tcagcctcgc cttcaataca tcaactggaa agcggggatt attcatcgcc
      781 acaatccacc actcgggatc aatttctgcc gccagtgccg cagggggggt gtggcggttg
      841 ctaagtgcgc cgaacttgaa aaatgtgaga ttgcttttcg ccagtgtact ccaccatata
      901 cgatccggtt gttttgccag tacatccagt tgtgctaacg catcggggtc gagtgcataa
      961 taaacacgta atgcttcatc aacctgcgca cgggagctat tttcctgcct cagaaaaaac
     1021 ttcgcccaaa tatcattgcg tggcgtgttt tgctgtaatt ccttgagaac aacagcgggt
     1081 aaattatccc aactaactaa tgcactctct aaaaagtccc agtcttccaa acgtatcagg
     1141 gcggcggcat ttaatagcat ctggtcatca acttcgccgt ggctaatcag ctcagacaaa
     1201 atcatatcag cgctggtttc atatgcatct gaatctgctt tcggattatt tttccgatgc
     1261 gataaa
//
LOCUS       DBKEKI010000319         1253 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.325, whole genome shotgun sequence.
ACCESSION   DBKEKI010000319 DBKEKI010000000
VERSION     DBKEKI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.325"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..232
                     /locus_tag="ACUQPX_004436"
     CDS             <1..232
                     /locus_tag="ACUQPX_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HHV9058845.1"
                     /translation="MATGAIERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQ
                     NYTAVMHFAGDCETVLALAVRFALAQLLQLLL"
     gene            complement(277..>1253)
                     /locus_tag="ACUQPX_004437"
     CDS             complement(277..>1253)
                     /locus_tag="ACUQPX_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HHV9058846.1"
                     /translation="PYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYHPDVRVWEIF
                     DHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQKPAAGEPAL
                     LLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHWATHPQVFAR
                     YARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEENEAMQDVDD
                     FELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADDGYQWFVEQG
                     GLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT          319 a          326 c          309 g          299 t
ORIGIN      
        1 aatggcaaca ggtgccatag agcgtgcgga tgctgatgtc agtattgcca ttaccggcta
       61 cggcggaccg gagggcggtg aagatggtac gccagcgggt accgtctggt tcgcgtggca
      121 tattaaaggc cagaactaca ctgcggttat gcattttgct ggcgactgcg aaacggtatt
      181 agctttagcg gtgaggtttg ccctcgccca gctgctgcaa ttactgctat aaccaggctg
      241 gcctggcgat atctccaggc cagccattgg tggtgtttat atgttcaagc cacgatgttg
      301 cagcatcggc ataatctgag gagccttacc gcgccattgt cgatgcaggc gttccagatc
      361 ttcgctgtta cctctggaaa ggatcgcctc gcgaaaacgc tgcccatttt cacgcgttaa
      421 tccgccctgc tcaacaaacc actggtaacc atcatcggcc aacatttgcg tccacagata
      481 agcgtaataa cctgcagcat atccgccacc aaaaatatgg gcgaaataac tgctgcgata
      541 gcgtggcggt atagcaggaa gatccatatt ttccgccacc agcgcccgca attcaaaatc
      601 atcgacatcc tgcattgctt cgttttcttc caggcaatgc cagcgcatat cgagaagtgc
      661 ggcgctaagc agttcgctca tctcataccc tttgttgaac aggctggcat tacgcatttt
      721 ctgttgcagt tcgtcaggca ttgctgcccc gctctgataa tgccgggcgt agcgagcgaa
      781 tacctgcgga tgcgttgccc agtgttcgtt gatttgcgac ggaaattcga caaaatcacg
      841 cggcgtgttg gtgccggaaa gcgtggcata acgctggcgg gcaaaaaggc cgtgcagcgt
      901 atgaccaaat tcatggaata aggttattac atcatcccag agtaacaacg caggctcacc
      961 ggcagcgggt ttctgataat tgcagacgtt ataaattacc ggatgcgttt cattaagcgt
     1021 tgattgctca acaaaattgc ccatccatgc accgccgctt tttgaatcac gggcgaagaa
     1081 atcaccgtaa aataacgcca gtcccacgcc attatgatca aaaatttccc acacacgaac
     1141 gtcaggatgg tagaccggaa tatcaaaacg ttcgacaaac ttaataccga agagctgatt
     1201 cgcggtccag aatacacctt catttaacac cgtgtttaat tcaaaatatg gct
//
LOCUS       DBKEKI010000320         1251 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.326, whole genome shotgun sequence.
ACCESSION   DBKEKI010000320 DBKEKI010000000
VERSION     DBKEKI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.326"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1202
                     /locus_tag="ACUQPX_004438"
     CDS             <1..1202
                     /locus_tag="ACUQPX_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HHV9058847.1"
                     /translation="QFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELI
                     KATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMD
                     ERGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARP
                     FVHIMQDKDIEENYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCV
                     WKTWAWETAFDQIREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPG
                     NKAILPILWSLFPHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLD
                     KTSGKFAEQKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIE
                     PLIVVKK"
BASE COUNT          315 a          294 c          353 g          289 t
ORIGIN      
        1 gccagtttga cggtaaatgg ctggatgaaa aagatccgct acaaaacgcc tatgtgcagg
       61 ccaacggtca ggtgatcaat caggatccgt atcattacta caccattacc gagagtgccg
      121 agcaggagct aattaaagcc accaacgagc tgcatctgat gtatcttcac gcaaccgaca
      181 aggtgctgaa agatgacaac ctgctggcgc tgttcgacat tccgaaaatc ctctggccac
      241 gtttgcgtct ctcctggcag cgtcgccgtc accatatgat cactggtcgt atggatttct
      301 gcatggatga gcgtggcctg aaggtttacg aatacaacgc cgactccgcc tcctgtcata
      361 ccgaagcggg cttgatcctc gaacgttggg cggagcaggg ctataaaggc aacggcttca
      421 atccggcgga agggctgatt aacgaactgg ctggtgcctg gaaacacagt cgtgcacgtc
      481 cgtttgtcca tatcatgcag gacaaagata tcgaggaaaa ctatcacgcg cagtttatgg
      541 agcaggctct gcaccaggcg ggctttgaaa cgcgtatctt gcgcgggctg gatgaactgg
      601 gctgggatgc tgccgggcaa ctgattgatg gggaagggcg actggttaac tgcgtgtgga
      661 aaacctgggc gtgggaaacc gcgtttgatc agattcgtga agttagcgac cgtgagtttg
      721 ctgcggtgcc aatccgtacc ggtcatccgc aaaacgaagt gcgtcttatc gacgtattgc
      781 tgcgcccgga agtgctggtc tttgagccgc tgtggacggt gatccccggc aacaaagcga
      841 ttctgccgat cctctggtcg ctgttcccgc accatcgtta cctgctggat accgatttca
      901 ctgttaatga tgaattggtg aaaacaggtt acgcagtgaa acctatcgcc ggtcgctgtg
      961 gtagcaatat cgacctggtc agccatcatg aagaggtgct ggacaaaacc agcggtaaat
     1021 ttgccgagca gaaaaacatc tatcagcaac tgtggtgttt gccgaaagtg gacggtaaat
     1081 acattcaggt atgtaccttc accgttggcg gcaactacgg tgggacgtgt ttgcgcggtg
     1141 atgaatcact ggtcatcaaa aaagagagtg atattgaacc gttaattgtg gtgaaaaagt
     1201 aatacatgtt gtagcaaccc cgtactgatt atgaagaata attagtacgg g
//
LOCUS       DBKEKI010000321         1241 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.327, whole genome shotgun sequence.
ACCESSION   DBKEKI010000321 DBKEKI010000000
VERSION     DBKEKI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.327"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(129..747)
                     /locus_tag="ACUQPX_004439"
                     /pseudo
     CDS             complement(129..747)
                     /locus_tag="ACUQPX_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816255.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
     gene            complement(985..1161)
                     /locus_tag="ACUQPX_004440"
                     /pseudo
     CDS             complement(985..1161)
                     /locus_tag="ACUQPX_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          407 a          245 c          206 g          383 t
ORIGIN      
        1 gacattttta tactctcgaa tatttttaaa atagaaacat ataattacga tataaatata
       61 aaaactaata ttaacttgac acttatctta aaaacactaa cgcaatctgc tttacaaaaa
      121 taaaaacaag taatacctaa agtcgtaacg acaccaatag tgttttcatc gacatgcttc
      181 aaaacctctt cagcattcat tatcaggcga tgatcataaa gtgaaatttt acgcaatttc
      241 acatcacagt aacggcagaa tttatgccag catatttgca caggaccgca aatcaggttc
      301 ggttgattta ccttgtactt cgcgtttttt acaccactgc cattttaacg ccaagcggcc
      361 gagcatagca gcttcagatg aaccaatggt tgagcaacct aaggtgtgtc gcgaatcggg
      421 gaaactccac gaatcggaaa gcatacgcac acagcgacct tcaggttccg ccatttgtgg
      481 atattcatct ttataaatca tgtttctatc aatagataaa tttagcaact cacggatttc
      541 atcatcaatc catgtttgac agaaagtagc taagttttgt cgtgaatccc catcaaggat
      601 gagctcatca cgtatggcgc tgaatacatt tcgagatttc ttctcatcta cacgcaaatg
      661 atatttatgc aatataaatg aaagctcaag tgagctatga atatcgaccg cttctctata
      721 ataagaatta cctgatttat ttttcataat aaacgccact caatataatc agattaaaca
      781 ttacaaacaa aatatggatt ttgcaatttt aaaactatca attttagata tgttttaatt
      841 aatataaaac tataaacata tcaaaaaatt actttattat ggtcatgata gatgtgtttc
      901 aatgccaacc tggtctattc ctacatatgt gagagcatat ttacaaaaaa atcgttattg
      961 tgatttcaac ggtgatgcat ccaattagta gaacatgcat ttttccatga atgccccgat
     1021 gactttttca tgaagctcaa tagaacgtga aaaacagagc gttttgcggg ccagccgttt
     1081 gattctggtt ctcagcgtca ggttattgcg ctcaatgcgc tgagtaaaaa tcttgccggt
     1141 caggtgcttt tctttcggaa gtgaagtggt caacaaaaac tggccaccgc gttagagttt
     1201 ttccagtatc gattttccga ttcgtttggg ggtaacccac c
//
LOCUS       DBKEKI010000322         1241 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.328, whole genome shotgun sequence.
ACCESSION   DBKEKI010000322 DBKEKI010000000
VERSION     DBKEKI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.328"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..689)
                     /gene="ipaH7.8"
                     /locus_tag="ACUQPX_004441"
     CDS             complement(<1..689)
                     /gene="ipaH7.8"
                     /locus_tag="ACUQPX_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HHV9058848.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRI"
     gene            1019..1198
                     /locus_tag="ACUQPX_004442"
                     /pseudo
     CDS             1019..1198
                     /locus_tag="ACUQPX_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          405 a          179 c          247 g          410 t
ORIGIN      
        1 ttaatcctgt aacttgaatc taattgtgaa aaactctccg gaagagtggt tatctgatta
       61 aaatgaaaat aatattcctt ttgtctgata ttgtttctat cagaaaattg aggaaaagta
      121 accagattat tgcgctgagc acagagaatt tccagtttat ccggtaagtc gggtaatgtt
      181 gtcagcctgt tataataaac atgtaaaaat ttcaattttt cgggaagagc cggtaagtta
      241 tatagcctgt tattttcaac aaataatgat gttaaatggt ttggcaaaac aggaaggttt
      301 tccagttgat tagaacgaac acttaaagtt gttaatgact caggaagttc tggtatcaca
      361 gaaagcctgt tattatcagc attaagttct ttgagaaatg caggcagctt tggcaatgat
      421 gttaatttat tattttctac aatcaaagca gaaatatgct ttggtaattc gggaagagac
      481 gtcaaattta ggtctgataa atttaatact gcttcttgat tctgaaagca ctgggagagt
      541 ctgttaaaag caatgcctcg ctcttccccg ggagaagagt tcttttccca ttcagtcagg
      601 attctcagat aatgttcatt actggttgag tttgagttaa tagaggggga gcaagaaact
      661 gatgagtgtg tattatttac agagaacata atgctaggtc aaccttatat ttgtgagaat
      721 aaatatttaa aattgtatat ttatacaaat ttcacttacc atttccgatg ttaagtttta
      781 atagtttcca ttttacacat aacagaagtt atgttgtaat ttatttattg aggttttcta
      841 tggaattctt tttttattgt gactgcagtc aataataaca ctgaattcaa cattccagtg
      901 agcaaggaaa tgtaatccgg gtaagtatat ttgttttaca tcatatttta gccaagctca
      961 tttattccta ttatagaatg ataaattaaa attactgtaa aaaaacgata cacatttaat
     1021 acgagggagg ccggcagaaa aacggcttgc ggtaaggcag gagaaatgtg tgccgcgaat
     1081 gaagtccttg tatgactgga taaagctcca gaggaaaact ttgtcaaacc atgctgaaat
     1141 ggtaaaggcg ttcaactata tcctgaatca ctggaatgca ctgaatgagt tttgccgggt
     1201 agtacttcca atcattagaa tatgtgcttt tcaatgaagg a
//
LOCUS       DBKEKI010000323         1223 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.329, whole genome shotgun sequence.
ACCESSION   DBKEKI010000323 DBKEKI010000000
VERSION     DBKEKI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.329"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..831)
                     /gene="caiA"
                     /locus_tag="ACUQPX_004443"
     CDS             complement(<1..831)
                     /gene="caiA"
                     /locus_tag="ACUQPX_004443"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HHV9058849.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     Q"
     gene            complement(862..>1223)
                     /locus_tag="ACUQPX_004444"
     CDS             complement(862..>1223)
                     /locus_tag="ACUQPX_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HHV9058850.1"
                     /translation="EQYGVARAIIETWAALPLSTATMWGFFILCFIATVTLVNACSYT
                     LAMSTCREVRDGEEPPLLVRIGWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVN
                     IMVTLSFIKDAKQNWKD"
BASE COUNT          292 a          346 c          306 g          279 t
ORIGIN      
        1 ctggaaacga ccaatagcct cgccaaactg cacgcgctgg ttggcgtagc gcgcagcatc
       61 ttcaaaggcg cacatcgccg taccgtagtt ggtcagggct accaggaaac gttcatggtc
      121 gaactcttct ttgacgcggt taaagccgtt accttcccga ccgaacatgt ctttctcatc
      181 cagttccaca tcgtcaaagg tgatttcaca gcagctatcc atacgcagac cgagcttctc
      241 aagtttggtc actttgatgc ccggtttgct catatcaaca aaccattcgg tgtagacagg
      301 tttgtccgga gaagccccgt cgcgcgccat caccacgatg tacggggtgt aggcgctact
      361 ggtaataaaa cacttactac cattaagata aatcttacca tttctacggg tataagtcgt
      421 tttcaggcta cccacgtcgg agcccgcgcc cggttcggta atcgctgagt tccacatctg
      481 cttaccggtg ccgcggaaag ccataatctt gtcgatctgc tcttgtgtgc cttcgcgcag
      541 gaaggtattg aacccgcccg gcagctggta cagtacatag gttggtgccc ccagacgtcc
      601 cagctccatc cacacggcgg cgagagtaac aaaccccgcg tccagaccac cgtgctcttc
      661 agggatcagc agactgtcga tacccatatc cgccagtgct ttgacaaaac gttccgggta
      721 gacgctgtca cggtcgcact cggcaaaata ggcctcccag ttttcgctgg ccatcagttc
      781 gcggataccg gcgacaaaca gttcctgctc atcatttaaa ttaaaatcca tctttcaacc
      841 tcttgatatt ttgggggtta attaatcttt ccagttctgt ttcgcgtctt taataaagga
      901 gagcgtcacc ataatgttga cgaagaacag cgggcatcct ccggcgataa tggcggtttg
      961 aatcggtttc aggccgccga gcgccagcag aacaataccg ataatgccaa ccagaattga
     1021 ccaaccgata cgcaccagca gcggtggttc ttcaccatcg cgtacttcgc ggcaagtaga
     1081 catcgccagg gtataagagc aggcgttaac cagcgtaacg gtggcaataa agcagaggat
     1141 gaagaagccc cacatggtgg cggtgctgag tggcagagcg gcccaggttt cgatgatggc
     1201 gcgcgccaca ccgtactgtt cga
//
LOCUS       DBKEKI010000324         1199 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.330, whole genome shotgun sequence.
ACCESSION   DBKEKI010000324 DBKEKI010000000
VERSION     DBKEKI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.330"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="ACUQPX_004445"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="ACUQPX_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HHV9058851.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNV"
BASE COUNT          341 a          214 c          259 g          385 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagtctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaataatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtc
//
LOCUS       DBKEKI010000325         1184 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.331, whole genome shotgun sequence.
ACCESSION   DBKEKI010000325 DBKEKI010000000
VERSION     DBKEKI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.331"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..330
                     /locus_tag="ACUQPX_004446"
     CDS             <1..330
                     /locus_tag="ACUQPX_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HHV9058852.1"
                     /translation="ISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNF
                     SFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYNEMV
                     DDYNSLR"
     gene            complement(380..>1184)
                     /locus_tag="ACUQPX_004447"
     CDS             complement(380..>1184)
                     /locus_tag="ACUQPX_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HHV9058853.1"
                     /translation="ITLLREQLDIPLFDSDLIGLYFACALERHQNERQPIILLSDQNA
                     IATINQLAIERDVLHCRVIIARSLSELVAIREEIDPLLIINNSHYLLDDAVNNYITVK
                     NIITAAGIEQIKHFLATAFIRQQPERFFSAPGSFHYSNVRGESWQHITRQICAQLVAQ
                     HHITADEAQRIIAREGEGENLIVNRLAIPHCWSEQERRFRGFFITLAQPVEVNNEVIN
                     HVLIACAAADARHELKIFSYLASILCQHPAEVIAGLTGYEAFMELLHKG"
BASE COUNT          337 a          261 c          291 g          295 t
ORIGIN      
        1 atttcagcga aacaaattaa taacctgata tcgcaagaga agtttgatgt tgatgcggca
       61 atgaagaaag tgtctgaact ggaaacgctg gtggcgcagg ccaaagaagc ggataaaggc
      121 ggcatgaatt tctcgtttat taattcggca ggccagtatc aactcgaggc taaaaaatac
      181 gttcgccgca tcagagataa agtcccgtac tctgactggg ataaagagca acttcaggat
      241 gcaaactcaa gctggatggt cgaagactct ttcccgagag cattacgcga gtacaacgaa
      301 atggttgatg actataatag cctgcgttaa acgtttttga tgataaatgc agtgtcggat
      361 gcggcgcatc cgacacagtt caccccttgt gaagtaactc cataaatgcc tcatatcctg
      421 ttaacccggc aataacctcc gccggatgct gacacaatat gcttgccaga tagctaaata
      481 tttttagctc atgacgcgca tcggcggcgg cgcaggcgat caagacatgg ttaatgactt
      541 cgttattcac ctcaactggt tgggcgaggg taataaaaaa tccacgaaaa cgtcgctcct
      601 gttcgctcca gcaatgtggg atggcgaggc gattaacaat caggttttca ccttcgcctt
      661 cgcgggcgat gatccgttgc gcttcatcgg cggtaatatg gtgttgtgcg actaattgcg
      721 cacaaatttg ccgggtaata tgttgccagc tttcaccgcg tacattcgaa taatgaaaac
      781 ttccgggggc agagaaaaaa cgctccggct gttggcgaat aaatgccgtc gccagaaaat
      841 gttttatttg ttcgataccg gcagccgtaa tgatattttt tacggtgatg taattattta
      901 ccgcgtcatc cagtaaataa tggctgttgt taatgatcaa taacggatca atctcttcgc
      961 gaatggcaac aagttcgctt aagctacggg caataatcac ccgacaatgt aaaacatcgc
     1021 gctcaatggc gagctgatta atagtggcaa tcgcgttctg gtccgagagc aaaatgatcg
     1081 gctggcgttc gttttgatgc cgttccagcg cacaggcaaa atacaaaccg atcagatcgc
     1141 tgtcgaaaag gggaatatcg agctgttcac gcagtagcgt aata
//
LOCUS       DBKEKI010000326         1182 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.332, whole genome shotgun sequence.
ACCESSION   DBKEKI010000326 DBKEKI010000000
VERSION     DBKEKI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.332"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..463)
                     /gene="pdeC"
                     /locus_tag="ACUQPX_004448"
     CDS             complement(<1..463)
                     /gene="pdeC"
                     /locus_tag="ACUQPX_004448"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HHV9058854.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYV"
     gene            892..1173
                     /locus_tag="ACUQPX_004449"
     CDS             892..1173
                     /locus_tag="ACUQPX_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HHV9058855.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          336 a          264 c          264 g          318 t
ORIGIN      
        1 ccacataatt tccccgctgc atataggtca ttttatagcc agagaaaaaa ggcgtatcgc
       61 ggtaataata gatagaaacg ttaggttcac gcttataatc ggccggtgca atcgtatagc
      121 cgtttacagg cgctatcagc gatgagcata aaaaatggtt atcacgggca tagatcaatt
      181 cattaatata aagatagcca cgaataatat tcaacattcg cttttgatgg gctggagtgc
      241 aaacttgccc ctgatagcgt tcagctgcat cgctcactaa atctgcctgg cgaatgacca
      301 gctcggattt atccagtgcc agttgagcaa aggttcggag ctgattattc acttctgatt
      361 ttgcccaaag gaaggcaatc catagcgaaa gaatgatggg aaagagaact aaaaagataa
      421 tgcccggcaa cgccagtaat tggtgtcgtg cacgatgact cattgctatc aatttcctgc
      481 ctgtttatac ccggccattg caaggtgaaa ccctggctac cgacgtcttt agataaatct
      541 tacggtataa atagcacttt tgcctgattt gaaattgata cctttttaat cgtcacctag
      601 atgaaaataa ttcattgata ttcaaaatta attttctaca ggaatacaga ggcggcggga
      661 agatatgccc ttaatgccac tgaaaaacca cataatctgt acagcaataa aagtcacggc
      721 ctaatatggc taacaaacaa tcaattgtga tatagttcac aaaattaatg aaacaaacag
      781 agtgtttcat ttttgtgttc tgcaagcccc gcgatagcca gagtgtacca cgcctcccgt
      841 gaacaacgcc gcgctgtcca gggcatcggc tcttttcaga ggaattgttt gatggctacc
      901 ctcaccactg gcgtggttct tcttcgctgg caacttctta gtgccgtaat gatgtttctg
      961 gccagcacac tcaacatccg ttttcgtcgg tctgattatg tcgggcttgc ggtgatcagc
     1021 agcggtctgg gcgttgtttc cgcctgttgg ttcgcaatgg ggttgcttgg catcacaatg
     1081 gcggacatca ccgctatctg gcacaacatc gagtcggtga tgatagaaga gatgaatcag
     1141 acaccgccac aatggccaat gattttgacc tgatacgtag aa
//
LOCUS       DBKEKI010000327         1179 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.333, whole genome shotgun sequence.
ACCESSION   DBKEKI010000327 DBKEKI010000000
VERSION     DBKEKI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.333"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..82
                     /locus_tag="ACUQPX_004450"
     CDS             <1..82
                     /locus_tag="ACUQPX_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HHV9058856.1"
                     /translation="YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            213..425
                     /locus_tag="ACUQPX_004451"
     CDS             213..425
                     /locus_tag="ACUQPX_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HHV9058857.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            669..1130
                     /locus_tag="ACUQPX_004452"
     CDS             669..1130
                     /locus_tag="ACUQPX_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HHV9058858.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
BASE COUNT          333 a          240 c          327 g          279 t
ORIGIN      
        1 gtatgcggga gcgcgtctgg cgaaaattcg tcgccagaac cggataaagg cagaacttca
       61 ggccgtgctt gatgagaaat aaaaaaagcc tcccccagag agaggaggca gggaaataag
      121 gcttaaaagg aaataacttc cgcttccgaa aggaagccag tccactgaat tatttatcac
      181 aataatactg acaactaaag ggggacaaga aaatgaatgg attcagaaac agttcacgga
      241 acggccaggt ctggcgttac cagcgtgccg gcgggcgggc cgttattctg gaagtcagtg
      301 gacgctggat ggaagcggca gaagcatggc gacgggctgc ctgtgttgcg cctaggacag
      361 actggcagca gtttgcccgg aaaagggctg aacactgcca ccggcgctgc cggggaaggg
      421 tgtaacgaaa aaagcagcct tcattactcc aggagtcaga aggctgaaag gagtttatga
      481 gatttgcttc ttaatatccg gtcgggcagt atgaccggac agttctgagt agcacagagt
      541 cttttccgca ttaagtcgat tttttatatt ttgtgattaa tttcacaaaa taaggtgtta
      601 ttcaatgtgt gctgcactat tcagggggtc tgaaatacag gtgccattta tctgatatgg
      661 agaataacat gaggaaatat attccactgg tattatttat cttttcatgg ccggtattgt
      721 ctgcagatat tcatggacgg gttgttcgtg tactggacgg tgataccatc gaggtcatgg
      781 actcactgaa ggcggtgcgg atcagactgg taaatattga tgcaccggaa aagaaacagg
      841 attatggccg gtggtcaaca gacatgatga aatcactggt cgcaggaaaa acggttaccg
      901 tcacatattt tcagcgagat cgttatggtc gtattctcgg gcaggtttat gcaccagacg
      961 gaatgaatat taatcagttt atggtccggg ccggggctgc ctgggtatat gagcagtaca
     1021 acacagatcc cgtgctacct gtccttcaga acgaagccag acaacaaaaa cgtgggctct
     1081 ggtcagatgc tgatccggtt cccccctgga tatggaggca ccgtaaataa agcgatggca
     1141 atcctatcat tcatctgttc tcagcgggaa taaaaatgg
//
LOCUS       DBKEKI010000328         1124 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.334, whole genome shotgun sequence.
ACCESSION   DBKEKI010000328 DBKEKI010000000
VERSION     DBKEKI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.334"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            289..>1124
                     /gene="garP"
                     /locus_tag="ACUQPX_004453"
     CDS             289..>1124
                     /gene="garP"
                     /locus_tag="ACUQPX_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HHV9058859.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LV"
BASE COUNT          272 a          263 c          259 g          330 t
ORIGIN      
        1 gaatgtcagg agtttaaacg cagaaatgtg atcaatgtca atcatttcag ggattgcagt
       61 gccgcccggc aaggtaaagc gcaaatctgt gctttagcgc gcaaccttat gtgcatatgc
      121 tcaaaataat atttaaaaag tattaattaa attagcattt gcacaatgat attctcgcgc
      181 tgcttatact aattcccgag ttcattgtcg ttacggcata aatgaaactt ataaataaca
      241 ccttccccat aataataaaa atcagcataa gtacccgagg taaataaaat gattctggac
      301 accgttgacg aaaaaaggaa aggtgtgcat acccgctatt taatattact gattattttt
      361 attgttaccg ccgttaacta cgccgatcgt gcaacgctgt ctattgctgg taccgaagtg
      421 gcaaaagagt tgcagttaag tgcggtttcg atgggttaca tcttctccgc ttttggctgg
      481 gcttacttgc tgatgcaaat ccccggcggc tggttgcttg ataagtttgg ctcgaaaaaa
      541 gtttacacct acagcctctt tttctggtcg ctgttcacct tcctgcaagg ctttgttgat
      601 atgttcccgc tggcctgggc agggatctcc atgttcttta tgcgctttat gctgggattc
      661 tcggaagctc catcattccc ggcgaacgcc cgtattgtcg ccgcctggtt cccgacgaaa
      721 gaacgtggta ctgcctccgc catctttaac tcggcacaat atttctcgct ggcgctcttt
      781 tcgcctctgc ttggctggct gactttcgcc tggggatggg agcacgtctt taccgttatg
      841 ggggtgattg gttttgtgct gacggcgctg tggatcaagt tgattcataa cccgacagat
      901 cacccacgta tgtctgagga agagctgaag tttatctctg aaaatggcgc ggtggtcgat
      961 atggaccaca aaaagccggg cagcgcggca gcaagcggtc ccaaactgca ttacatcaag
     1021 caattgctct ctaaccgcat gatgttgggc gtatttttcg gacaatattt tatcaacacc
     1081 atcacctggt tcttcctcac ctggttcccg atttatctgg tgca
//
LOCUS       DBKEKI010000329         1111 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.335, whole genome shotgun sequence.
ACCESSION   DBKEKI010000329 DBKEKI010000000
VERSION     DBKEKI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.335"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..120
                     /locus_tag="ACUQPX_004454"
     CDS             <1..120
                     /locus_tag="ACUQPX_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058860.1"
                     /translation="NIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            303..497
                     /gene="bfd"
                     /locus_tag="ACUQPX_004455"
     CDS             303..497
                     /gene="bfd"
                     /locus_tag="ACUQPX_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HHV9058861.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            570..1046
                     /gene="bfr"
                     /locus_tag="ACUQPX_004456"
     CDS             570..1046
                     /gene="bfr"
                     /locus_tag="ACUQPX_004456"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HHV9058862.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1043..>1111)
                     /locus_tag="ACUQPX_004457"
     CDS             complement(1043..>1111)
                     /locus_tag="ACUQPX_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HHV9058863.1"
                     /translation="PFGPFLAAAGFVIGWDSLLIGR"
BASE COUNT          319 a          235 c          262 g          295 t
ORIGIN      
        1 aacatcaaaa tggttgttac cctgatccac ccgatcgcga tggacgacgg tctgcgtttc
       61 gcaatccgtg aaggcggccg taccgttggc gcgggcgttg ttgctaaagt tctgggctaa
      121 ttacacgtta attagttttg aattgaaaag ggcgcttcgg cgcccttttt gctgcccgta
      181 atttgagctc gaaatattta gtcgtaattt tgctcgctga actatggcgc aaataaaact
      241 gtgctattgt aatcataacc attctcattt acactttgcg cagaatttga acgggagcaa
      301 tcatgtacgt ttgtttgtgt aatggtgtaa gtgacaaaaa aattcgccag gcagtccgtc
      361 agtttcatcc gcaatctttc caacaattgc gtaagtttat tcctgtggga aatcaatgcg
      421 gtaagtgcat tcgcgccgcg cgcgaagtga tgcaggacga gttgatgcag atgccagaat
      481 ttaaagagat tgcctgaagc ccacgcttat tttttgacat cccgctagcc ccatctacgc
      541 tctaatagtg gaagcggagg gactataaaa tgaaaggtga tgttaaaatc ataaattatc
      601 tcaataaact attgggaaat gagcttgtcg caatcaatca gtattttctc catgcccgga
      661 tgtttaaaaa ctggggcctg atgcgtctca atgacgtcga ataccatgaa tccatcgatg
      721 agatgaaaca cgctgataaa tatatcgagc gtattttgtt tttagaagga atccccaact
      781 tacaggattt aggcaaactg gggattggtg aagatgtcga ggagatgctg caatctgacc
      841 tgcgattaga actggaaggc gcgaaggatc tgcgtgaggc tatcgcatat gccgacagcg
      901 ttcatgacta tgttagccgc gatatgatga tcgagatcct tgctgatgaa gaaggtcata
      961 tcgactggct cgaaactgaa ctggatctca tcggtaaaat cggcctacaa aattacctgc
     1021 aatcacaaat taaagttaaa gattaacggc ctatcagcaa actatcccag cctataacga
     1081 aacccgcagc cgccagaaat ggcccaaaag g
//
LOCUS       DBKEKI010000330         1110 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.336, whole genome shotgun sequence.
ACCESSION   DBKEKI010000330 DBKEKI010000000
VERSION     DBKEKI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.336"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            94..>1110
                     /locus_tag="ACUQPX_004458"
     CDS             94..>1110
                     /locus_tag="ACUQPX_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HHV9058864.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     GNPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFP
                     GGKHG"
BASE COUNT          302 a          286 c          297 g          225 t
ORIGIN      
        1 cgcggaatag tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc
       61 caaatcaaaa caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt
      121 gacgaacgac gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc
      181 agactgactg ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg
      241 ctctgctgcg cccgctcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt
      301 gagggactga aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc
      361 acactgttac gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc
      421 tggagtacag aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc
      481 ggaaccgttc gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact
      541 ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac
      601 gaatgcagcg cagaacatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc
      661 aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag
      721 aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt cagctatgtg
      781 ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca
      841 taccgtcggg cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg
      901 gaaacacaga gctggctgaa ggggaacccg aagttcaggg tcatttatca gccggtttac
      961 tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt
     1021 aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc
     1081 gtcagcccat tccccggagg caaacatgga
//
LOCUS       DBKEKI010000331         1108 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.337, whole genome shotgun sequence.
ACCESSION   DBKEKI010000331 DBKEKI010000000
VERSION     DBKEKI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.337"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..390
                     /locus_tag="ACUQPX_004459"
     CDS             <1..390
                     /locus_tag="ACUQPX_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HHV9058865.1"
                     /translation="VCGDEMSIQRIFSRTHIVYACTGEGDDGYFKTGRTFADEHYLKS
                     RVTVVDVSDPDVLALLKELEVKDKRIAELTDVLTQMINAHKTTIRFGHERITECGGDC
                     DSPEKMISENPDIRMAEAVLRAGIKTE"
     gene            488..795
                     /locus_tag="ACUQPX_004460"
                     /pseudo
     CDS             488..795
                     /locus_tag="ACUQPX_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367210.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            877..1020
                     /locus_tag="ACUQPX_004461"
                     /pseudo
     CDS             877..1020
                     /locus_tag="ACUQPX_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
BASE COUNT          335 a          212 c          292 g          269 t
ORIGIN      
        1 gtttgtggcg atgaaatgtc gatacagcgc atattttcca gaacgcatat tgtgtatgcc
       61 tgcacaggtg agggggatga tggatatttt aaaactggtc gaacttttgc ggatgagcat
      121 tacctgaaat cgcgcgtaac cgtcgttgat gttagcgatc ctgacgtact ggctttactg
      181 aaagagctgg aagttaaaga caaacgcatt gcagaattga cagatgtgct tacacaaatg
      241 attaatgcgc acaaaaccac aattcgtttt ggtcatgaac gcataactga atgtggtggt
      301 gattgcgact cgccggaaaa gatgatttca gaaaatccgg atatcagaat ggcagaggct
      361 gttttgagag caggaataaa aactgaataa ttaaatttag cactgtaaat aaaatttaat
      421 ccttaaccgg agggatttct gcaccctcag aacatcagga ggccgcccga aatggcggta
      481 atgaaaaatg actgaattaa caaaagagca attaatcgaa gaagctaaat taaaaatagc
      541 gattgcgaaa tgccatccca attcaggcgt ggcacgagta gagggtgagt tattcaaaat
      601 tgcactggca tcgctggaag ctgagccggt ggcgtggaag gcaatcttca cgcaaattga
      661 ccatgaatat aatacgttca ccgctctgta ttctgacaaa gcagaagcag aacggtgggt
      721 gcgactgcat aaagtaggtg attttcgggc ataacaccac tttatgcagc atcgccagcg
      781 ctggtagtgc ctgataagtt gccgcgtgaa tacataagag gttggcctct tgcgtatagt
      841 gattatgctg aaggatggaa cgattgtcgt gcagccatgc ttcagggatg gcagccatgc
      901 catcaaatta ccgcctcaat ggcaaaagcc attgcgctta aacttggtgc tgaactgaat
      961 aacgaagaag cagagatttt cgcggatggg tataacgccg ccatgcttca tgccggaaac
     1021 tgaaccgtag caggaggtga atcgatgagc tggcctgaag cattcacaac ggtaggaatt
     1081 gcaatggcgg tggcgctggt tgtgtatt
//
LOCUS       DBKEKI010000332         1101 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.338, whole genome shotgun sequence.
ACCESSION   DBKEKI010000332 DBKEKI010000000
VERSION     DBKEKI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.338"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..534
                     /locus_tag="ACUQPX_004462"
                     /pseudo
     CDS             1..534
                     /locus_tag="ACUQPX_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            901..1101
                     /locus_tag="ACUQPX_004463"
                     /pseudo
     CDS             901..1101
                     /locus_tag="ACUQPX_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          344 a          239 c          225 g          293 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggcg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      661 tcatattctg aggattaact acataacaag gcgcaagata tatatccaaa aacaaataat
      721 accgttgcaa taattccttt cagtaaagga ggtgtgatta atttcagggg atttcagagt
      781 gaaaattgta caacatatta aaatatgttc atttcgttat aatacacaca agaaaatatt
      841 aagttagata atatattcta aataaccata gataaaaacg cacataatgg ggaatatttt
      901 atgaaaatac ctgaagcagt aaatcatatt aatgttcaga ataatattga tcttgttgat
      961 ggaaaaacga atccgaataa agcaacaaaa gcattacaaa aaatatatta cgcgtaacaa
     1021 actcatcttc ttctggcata agtgaaaaac atttagatca ctgtgctaat actgtgaaaa
     1081 actttttaag gaagtctata g
//
LOCUS       DBKEKI010000333         1101 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.339, whole genome shotgun sequence.
ACCESSION   DBKEKI010000333 DBKEKI010000000
VERSION     DBKEKI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.339"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..514
                     /locus_tag="ACUQPX_004464"
     CDS             <1..514
                     /locus_tag="ACUQPX_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HHV9058866.1"
                     /translation="LQAKFGGQEFLIGLDLALLLGHTAVVSASLIFIPLTILIAVCVP
                     GNQVLPFGDLATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAA
                     NAGALKAGGMVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGF
                     TKQEKAVLAE"
     gene            562..>1101
                     /locus_tag="ACUQPX_004465"
     CDS             562..>1101
                     /locus_tag="ACUQPX_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HHV9058867.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCA"
BASE COUNT          241 a          227 c          290 g          343 t
ORIGIN      
        1 tttacaggcg aagttcggcg gtcaggagtt cctgattggc ctggatctcg cattactgct
       61 ggggcatacg gcggtggtat cggcaagcct gatttttatt ccgctcacca ttttaattgc
      121 tgtttgtgtt ccgggtaatc aggtactgcc gtttggcgat cttgccacaa tcggcttctt
      181 cgtggcgatg gcggtcgcgg tgcatcgtgg aaatctgttc cgcaccttaa tctcgggtgt
      241 catcattatg agcatcaccc tgtggatcgc gacgcaaact attggtttgc acacccaact
      301 ggcggctaat gctggggcgt taaaagccgg gggtatggtg gcttcaatgg atcagggcgg
      361 ttctcccatt acctggttac tgattcaggt tttctccccg caaaatattc ccggtttcat
      421 tattatcggc gcaatttatc tgaccggtat tttcatgacc tggcgtagag cgcgtggttt
      481 tactaaacaa gagaaagccg ttctcgcaga ataattttta cctgaggggg aggttatccc
      541 ccttaacaat caggagtttt tatgaaatca gtagtgaatg atactgatgg tatcgtgcgc
      601 gttgcaaaaa gcgtcattcc tgaaattaaa catcaggatg aggtgcgggt aaaaattgcc
      661 agctcgggct tatgtggttc cgatttaccc aggatattta aaaatggtgc acattattat
      721 ccaataacgt taggccatga atttagcggc tatattgatg cggtgggatc cggtgttgat
      781 gatttacacc ctggcgatgc ggttgcctgt gtgccgttat taccctgttt tacttgtcca
      841 gagtgtttga aagggtttta ttcccagtgc gcaaaatatg attttattgg ctcgcggcgt
      901 gatggtggat ttgctgaata tattgtcgtt aagcgaaaaa atgtctttgc tctacccacg
      961 gatatgccta ttgaggatgg ggcttttatt gagccgatta ccgttggcct gcatgctttt
     1021 catttagcgc aaggttgtga gaataaaaac gttattatta ttggtgccgg aaccattggc
     1081 ctgctggcta ttcagtgcgc t
//
LOCUS       DBKEKI010000334         1069 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.340, whole genome shotgun sequence.
ACCESSION   DBKEKI010000334 DBKEKI010000000
VERSION     DBKEKI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.340"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..1069
                     /locus_tag="ACUQPX_004466"
                     /pseudo
     CDS             1..1069
                     /locus_tag="ACUQPX_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          261 a          262 c          324 g          222 t
ORIGIN      
        1 ttgatgtctt agggccggag aaacgcagac ggcgtaccac acaggaaaag atcgcaattg
       61 ttcagcagag ctttgaaccg gggatgacgg tctccctcgt tgcccggcaa catggtgtag
      121 cagccagcca gttatttctc tggcgtaagc aataccagga aggaagtctt actgctgtgg
      181 ccgccggaga acaggttgtt cctgcctctg aacttgctgc cgccatgaag cagattaaag
      241 aactccagcg cctgctcggc aagaaaacga tggaaaatga actcctcaaa gaagccgttg
      301 aatatggacg ggcaaaaaag tggatagcgc acgcgccctt attgcccggg gatggggagt
      361 aagcttagtc agccgttgtc tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg
      421 aaccgatgac tggatggatg gccgccgcag tcgtcacact gatgatacgg atgtgcttct
      481 ccgtatacac catgttatcg gagagctgcc cacgtatggt tatcgtcggg tatgggcgct
      541 gcttcgcaga caggcagaac ttgatggtat gcctgcgatc aatgccaaac gtgtttaccg
      601 gatcatgcgc cagaatgcgc tgttgcttga gcgaaaacct gctgtaccgc catcgaaacg
      661 ggcacataca ggcagagtgg ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt
      721 cgagttctgc tgtgataacg gagagagact gcgtgtcacg ttcgcgctgg actgctgtga
      781 tcgtgaggca ctgcactggg cggtgactac cggcggcttc aacagtgaaa cagtacagga
      841 cgtcatgctg ggagcggtgg aacgccgctt cggcaacgat cttccgtcgt ctccagtgga
      901 gtggctgacg gataatggtt catgctaccg ggctaatgaa acacgccagt tcgcccggat
      961 gttgggactt gaaccgaaga acacggcggt gcggagtccg gagagtaacg gaatagcaga
     1021 gagcttcgtg aaaacgataa agcgtgacta catcagtatc atgcccaaa
//
LOCUS       DBKEKI010000335         1065 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.341, whole genome shotgun sequence.
ACCESSION   DBKEKI010000335 DBKEKI010000000
VERSION     DBKEKI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.341"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..949
                     /locus_tag="ACUQPX_004467"
     CDS             <1..949
                     /locus_tag="ACUQPX_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9058868.1"
                     /translation="PNYFLINIATEQVAPLKAFLAEHHIVPESFYPVVRARLTAINDK
                     PTEGNEDEALNRELNLTWQNTRPDHNPIVAGNWPPKADEVSMEEGLAKRLNVALGDTV
                     TFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFPEGALDGQPQSWLTSFRWENGNGMLT
                     QLNRQFPTISLLDIGAILKQVGQVLEQVSRALEVMVVLVTACGMLLLLAQVQVGMRQR
                     HQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSGLVAAIGAETALAVLQAKVFDFPWEP
                     DWRLWIVLPCSGALLLSLFGGWLGARLVKGKALFRQFVG"
BASE COUNT          226 a          245 c          339 g          255 t
ORIGIN      
        1 cccgaactac tttttaatta acatcgccac agaacaggta gcaccgctaa aagcgttcct
       61 cgcggaacat catatagtcc cggaatcatt ttatccggta gtgcgggcgc ggctgacggc
      121 gattaacgat aagccgacag aaggtaatga agatgaggcg cttaatcgcg aactcaatct
      181 tacctggcaa aatacgcggc ccgatcataa tccgattgtc gccggtaact ggccgccaaa
      241 agccgatgaa gtgtcgatgg aagaggggct ggcgaaacgc ttaaacgttg ccctcggtga
      301 taccgtgact tttatgggcg atacccagga gttccgcgct aaagtgacca gcctgcgcaa
      361 agtggactgg gaaagtctgc ggcctaattt ctattttatt ttccctgaag gggcattaga
      421 cgggcaaccg cagagctggc ttaccagttt ccgctgggag aatggcaacg gcatgttgac
      481 gcaactcaac cgccagttcc cgaccattag cctgttagac attggcgcga ttttaaaaca
      541 ggtcggtcag gtgctggagc aggtaagtcg ggcgctggag gtgatggtgg tgctggtcac
      601 cgcctgcggt atgttgctgt tgctggcaca ggtgcaggtg ggtatgcgcc agcgtcatca
      661 ggagctggtg gtatggcgca cactcggtgc ggggaaaaaa ctgctgcgca cgacattgtg
      721 gtgtgagttc gccatgcttg ggtttgtttc cggcctggtg gccgcaattg gtgcggaaac
      781 ggcactggca gtgttgcagg cgaaagtgtt tgatttcccg tgggagccag actggcgatt
      841 gtggattgtt ctgccgtgca gcggtgcgct gctgctgtcg cttttcggcg gctggctggg
      901 tgcgcgactg gttaagggta aggcgctgtt caggcagttt gtggggtgat gacaggtgtt
      961 agattgcctg atgcgctacg cttatcaggc ctacgaagag cgtgcaatat attgattttg
     1021 caagattgtg taggccggat aaggcgttta cgccgcatct ggcat
//
LOCUS       DBKEKI010000336         1059 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.342, whole genome shotgun sequence.
ACCESSION   DBKEKI010000336 DBKEKI010000000
VERSION     DBKEKI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.342"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..768)
                     /locus_tag="ACUQPX_004468"
     CDS             complement(<1..768)
                     /locus_tag="ACUQPX_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHV9058869.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLIDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECV"
     gene            868..>1059
                     /locus_tag="ACUQPX_004469"
     CDS             868..>1059
                     /locus_tag="ACUQPX_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HHV9058870.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDK"
BASE COUNT          307 a          239 c          238 g          275 t
ORIGIN      
        1 tacacattct aaacgaccag tatcaaggta ctccttgatc tgttcataga gcagataccc
       61 gataccagca cccatcaaaa cggcctccag ttcagcatca acatcatcca gaatgatatt
      121 tcctgcgaca gcaatttcca gctcctttgc aaattgccag tggaaaggtt tgccgctggg
      181 ataacgaaaa acaacacatt gatgattaag cagatcatga ggatggcggg gtttgccata
      241 tcttgcaaag tactctggcg ttgctgctac acataatttc actggtgggc cgatcgccac
      301 gctgatcata tctttttcga cgatgccact aagtcgaacg ccagcgtcaa agccctgttg
      361 gacgatatcg ataagagaat cgtcagttgt gagctctact ttaatatcag gatattcacg
      421 tgtgaaacca accagtaaag gcattaaaaa tatacgtgca gctacccggg cggcattgat
      481 tttgagcgtt cctgtcggtg tcagacgaaa atcattcatc tcatctaaca tgatctgaat
      541 ttcgtcgaaa gctgggcgca atcgttcata gagattagaa cccgcttccg ttaacgatac
      601 gcttcgggtt gtccgattga ataagcgaat tttaagacgt tgttccagtg ttttaatact
      661 atggctaatg gcggacgagg ataagcctaa ctcatcgcct gctgcacgaa agctttgatt
      721 acgggccacg gcaaagaaag tggcaaagtc agacatattc atccgcatta gtgaaaatcc
      781 ttcattaact catcctgaat catcacgttt acaggattat gccgcctgcg taaagtgcgc
      841 tccagaactt aacgtggagg taaaattatg cagtctgaac gtatttattt ggtatgggcc
      901 catcctcgtc atgattcatt gaccgcaaat attgctgatg cgatccatca gcgggcaatg
      961 gagcggaaaa tacaggtgac ggaactcgat ttatatcggc gtaatttcaa cccggtgatg
     1021 acgtcggaag atgaaccaga ctggaagaat atggataaa
//
LOCUS       DBKEKI010000337         1045 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.343, whole genome shotgun sequence.
ACCESSION   DBKEKI010000337 DBKEKI010000000
VERSION     DBKEKI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.343"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(81..563)
                     /locus_tag="ACUQPX_004470"
     CDS             complement(81..563)
                     /locus_tag="ACUQPX_004470"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHV9058871.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(554..856)
                     /locus_tag="ACUQPX_004471"
     CDS             complement(554..856)
                     /locus_tag="ACUQPX_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HHV9058872.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          309 a          256 c          210 g          270 t
ORIGIN      
        1 tttttctgct cattattggc tccttctgat gccattctat ttcaggaagg agtgtccgtt
       61 aaactcaggc tacctcagac tcagacttta taaaacaagg tattaggtgc cacaactgac
      121 tgaacaaaac caaaacgctc ataaaatgct ctggcgtcat catcaattgc atgtaccata
      181 acagccttaa tgccaacctg atcagccaga ttgacaaccc ggtgaatggc atcacttagt
      241 agccatttac cgaaaccatg cccctgggta caaacatcaa cagccagacg ccctaacaga
      301 acaacaggga caggattggg catgttatgc cgcaaactgc gaccaagctc ggcatgagtg
      361 acggaaccag tagctaatga gtagtatccc acaacacgta aagtatcctc caagcaaata
      421 acgaacgtgc gagaagcatt gagcatctgg tttttcatag cccgaccacg taaccagtta
      481 cttagcacct catttccaca gtcaaatggc tgaagtatat gctcatccgt caacaatgct
      541 ggcgcggtga catttatttc cattcaggct ttaccgccat cagacgttca cgcccttctg
      601 cattctgcac aggagcttcc agttgcgtaa taaacgaatc ataacgttcg ttatccagca
      661 caaataaacg ttgatccagg ataatgtttt tagcttcctg gtacgccagt tccatgatga
      721 aatcggtacg gttcttattg accaagctcg cagcataatc aataacggct cgttcctcat
      781 cagttgcccg gatgttaatc tgatgttctc gcggtgtttt acggacgctt gcagctgtag
      841 acataaaaac ctccattggt aacaatttgc tatgcaatta atgttatcgc aaatggtaac
      901 agaacgctat acagaatcac ttacgggaaa agcgatcccc tgataaaaac aatggcagag
      961 acctgaacat tgtgacgatg atctgtgtcg gcgcacaaat catgtctgat tacggccgtt
     1021 taatgtgatt ttgataatga aatgc
//
LOCUS       DBKEKI010000338         1007 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.344, whole genome shotgun sequence.
ACCESSION   DBKEKI010000338 DBKEKI010000000
VERSION     DBKEKI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.344"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            42..551
                     /gene="dfrA8"
                     /locus_tag="ACUQPX_004472"
     CDS             42..551
                     /gene="dfrA8"
                     /locus_tag="ACUQPX_004472"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA8"
                     /protein_id="HHV9058873.1"
                     /translation="MIELHAILAATANGCIGKDNALPWPPLKGDLARFKKLTMGKVVI
                     MGRKTYESLPVKLEGRTCIVMTRQALELPGVRDANGAIFVNNVSDAMRFAQEESVGDV
                     AYVIGGAEIFKRLALMITQIELTFVKRLYEGDTYVDLAEMVKDYEQNGMEEHDLHTYF
                     TYRKKELTE"
     gene            548..>1007
                     /locus_tag="ACUQPX_004473"
     CDS             548..>1007
                     /locus_tag="ACUQPX_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HHV9058874.1"
                     /translation="MTFVAISDTHLHNWSQFAIPTESGINSRLLQILKAIEEAACAAD
                     YHAPAGVVPTVYHGGDLFHVRGSLTPSVLNAVLDFFKTIHRDYGVRFRMIAGNHDLET
                     KDSCPMGNAAAALNSLPFVEVVSEKTLFEDHKVALLPWRDSMDDLRADLAH"
BASE COUNT          244 a          266 c          260 g          237 t
ORIGIN      
        1 acgcaataat caccaattcc tcttttgata ggtaaacaac aatgatcgag cttcatgcca
       61 ttttagctgc caccgccaat ggttgcattg ggaaggacaa cgcacttccc tggccaccac
      121 taaaaggcga tctggccaga ttcaaaaaat tgaccatggg gaaggtggtc attatggggc
      181 gcaagaccta tgagagcttg cccgtcaaat tagaaggtcg cacctgcatc gttatgacgc
      241 gccaagcgct ggagcttccg ggtgttcgtg acgctaacgg cgctatcttc gtgaacaacg
      301 tcagcgacgc catgcggttc gctcaagaag agagcgtggg cgatgtggcc tacgtcattg
      361 gtggcgctga gatattcaag cgacttgcct tgatgatcac gcagattgaa ttgacctttg
      421 ttaagcgact gtacgaaggc gacacctacg ttgatctggc cgaaatggtc aaagactacg
      481 agcagaatgg catggaagaa catgaccttc acacttactt cacttaccgt aaaaaggagc
      541 ttacagaatg accttcgttg ccatttcaga tacgcacctg cacaactggt cgcagttcgc
      601 gataccaaca gagtcgggca ttaactccag gctgttgcag attcttaaag ccattgaaga
      661 agcggcttgc gccgccgatt accacgcccc agcgggcgtg gttccaaccg tctatcacgg
      721 cggcgatctc ttccatgtgc gaggttcttt gaccccttct gtgctaaacg cggtgctcga
      781 cttttttaag accatccacc gtgactacgg cgttcgcttt cgcatgatcg ccggcaatca
      841 cgatctggaa accaaagact catgtcctat gggcaatgca gctgcggcgc ttaattcgtt
      901 gccgtttgtg gaggttgtat ccgaaaaaac gctgttcgag gaccacaaag tcgctctgct
      961 gccatggcgc gattccatgg acgatctgcg tgcggatctt gcgcacg
//
LOCUS       DBKEKI010000339          982 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.345, whole genome shotgun sequence.
ACCESSION   DBKEKI010000339 DBKEKI010000000
VERSION     DBKEKI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.345"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(19..822)
                     /gene="dkgB"
                     /locus_tag="ACUQPX_004474"
     CDS             complement(19..822)
                     /gene="dkgB"
                     /locus_tag="ACUQPX_004474"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HHV9058875.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          258 a          219 c          222 g          283 t
ORIGIN      
        1 aggagctgtc agagaggctt aatcccattc aggagccaga ccttccgggc taactaggcg
       61 gtcgttgcaa tccagtgcgg cgatcgcttt tttatcttcg gcatcaagct gtaaattttg
      121 tgccttaaga ttactttcca ggtttttacg tttagtagaa gaaggaatta ctgagtaacc
      181 ttcccccata gcccacgcca gaatcacttg tgccggaatc gcattgtgtt tagctgcaat
      241 acgagcaata acctcatctt tcagtgcctt accgtaagcc agcgtcatat aggaagtgat
      301 atgaataccg tgctgtttag cccaggcaac cactttacgg ttttgcagat aaggagagag
      361 ttcaatctgg ttggtagcga tgttttcagc gccaacagca gcaatcgctt tttccatcaa
      421 tgggatcgtg aagttggaaa taccgatctc acgcgtcagc ccttgttttt tggcttccag
      481 cagcgcctgc ataaactctt caacagagac ttcatcgttt ggtgacggcc agtggattag
      541 cgtcagatca acataatcgg tacgcaattt ttgcaggctc tctttcagac tcgggatcaa
      601 tttgtctttg ctgagatttt caatccagat tttagtggtg atgtagagtt catgacgtgg
      661 cacgccactt tctgcaatcg cctgacctac tgcggcttcg ttatcataga tttgtgcggt
      721 atcaattgcg cgataaccaa gttcaagcgc cgttttcaca gatgaaataa caacgtcgtc
      781 tttcagacgg aaagtaccta aaccaaatgc agggatagcc ataatatgcc tcttttaatg
      841 ctattttcgt taactgaggt gattatgggc ttcagactgt tgcggaaaaa gagcgtaaaa
      901 cgcagaggat ttttgcgatt ctagcaataa tagatatcca gacgaaaaaa aacctcgagc
      961 gttaactcga ggcttcttaa ta
//
LOCUS       DBKEKI010000340          978 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.346, whole genome shotgun sequence.
ACCESSION   DBKEKI010000340 DBKEKI010000000
VERSION     DBKEKI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.346"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(10..150)
                     /gene="yqcG"
                     /locus_tag="ACUQPX_004475"
     CDS             complement(10..150)
                     /gene="yqcG"
                     /locus_tag="ACUQPX_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HHV9058876.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            289..960
                     /gene="queE"
                     /locus_tag="ACUQPX_004476"
     CDS             289..960
                     /gene="queE"
                     /locus_tag="ACUQPX_004476"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HHV9058877.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          278 a          201 c          229 g          270 t
ORIGIN      
        1 aatttctcat taaatctgga taaataaatt gacggcggca tcggtaattt tagattcgtt
       61 atcattaaaa actaatacag aaaaaataaa tataattttg gtgaatgttt tgacatgatt
      121 aaatccattt tctttatttt cttctgacat aatacgttac tccctttaat tattaacaat
      181 atgtttcgta tttttcttta aatctgcatg taacagaatg ttaataagac atgattgttg
      241 cggcaggatc tgcaataatt aacaattatc cacttcacag agaatgctat gcagtacccg
      301 attaacgaga tgttccagac cctgcaaggt gagggttact ttaccggcgt ccccgccatt
      361 tttattcgtt tacagggatg cccggttggc tgtgcctggt gcgacaccaa acacacctgg
      421 gaaaagcttg aggatcggga agtctccctt ttcagcattc tggcgaagac caaagagagt
      481 gataagtggg gggcggcgag cagtgaagac ttgctggctg tcattggtcg tcagggatat
      541 accgcgcggc atgtggtgat tacgggtggt gagccttgca ttcatgattt gctgccactg
      601 actgacctgc tcgaaaagaa cggttttagc tgccagatcg aaaccagtgg aactcatgaa
      661 gtacgctgca caccgaatac ctgggttacc gtatcgccaa agctgaacat gcgcggcggc
      721 tatgaagtgt tgtcacaggc actggagcga gccaacgaaa tcaagcatcc agtggggcgc
      781 gtacgcgata ttgaagcact ggatgaacta ctggcaacgc tgaccgatga taaaccgcga
      841 gtcattgcac tgcagccgat tagccaaaag gatgatgcca cacgattgtg cattgaaacc
      901 tgtattgcgc gtaattggcg tttgtcgatg caaacacata aatatttaaa tattgcctga
      961 ttacacattt ataagcgt
//
LOCUS       DBKEKI010000341          976 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.347, whole genome shotgun sequence.
ACCESSION   DBKEKI010000341 DBKEKI010000000
VERSION     DBKEKI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.347"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            225..>976
                     /gene="folD"
                     /locus_tag="ACUQPX_004477"
     CDS             225..>976
                     /gene="folD"
                     /locus_tag="ACUQPX_004477"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HHV9058878.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAARLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIHG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAA"
BASE COUNT          242 a          262 c          249 g          223 t
ORIGIN      
        1 atcttaatcc ttaaacaagg aaataaatca gttccagatt tacaacgcca tcatgtacga
       61 aaaatgaagc tttcaatctc agcgacctca gcgacggtgc gcctaacctt cgcaagaggt
      121 cgcttcacgc gataaatctg aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag
      181 acgcatcccc ttcgacctac gtaacagatg gaattctctc tctgatggca gcaaagatta
      241 ttgacggtaa aacgattgcg cagcaggtgc gctctgaagt tgctcaaaaa gttcaggctc
      301 gtattgcagc cagactgcgg gcaccaggac tggccgttgt gctggtgggt agtaaccctg
      361 catcgcaaat ttatgtcgca agcaaacgca aggcttgtga agaagtcggg ttcgtctccc
      421 gctcttatga cctcccggaa accaccagcg aagcggagct gctggagctt atcgatgcgc
      481 tgaatgccga caacaccatc gatggcattc tggttcaact gccgttaccg gcgggtattg
      541 ataacgtcaa agtgctggaa cgtattcatc cggacaaaga cgtggacggt ttccatcctt
      601 acaacgtcgg tcgtctgtgc cagcgcgcgc cgcgtctgcg tccctgcacc ccgcgcggta
      661 tcgtcacgct gcttgagcgt tacaacattg ataccttcgg cctcaacgcc gtggtgattg
      721 gcgcatcgaa tatcgtcggt cgcccaatga gcatggaact gttgctcgca ggttgcacca
      781 ccaccgtgac tcaccgcttc actaaaaatc tgcgtcatca cgtagaaaat gccgatctgt
      841 tgatcgttgc cgttggcaag ccaggcttta ttcacggtga ctggatcaaa gaaggcgcaa
      901 ttgtgattga tgtcggcatc aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt
      961 ttgaagacgc ggctaa
//
LOCUS       DBKEKI010000342          968 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.348, whole genome shotgun sequence.
ACCESSION   DBKEKI010000342 DBKEKI010000000
VERSION     DBKEKI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.348"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(222..377)
                     /gene="hokD"
                     /locus_tag="ACUQPX_004478"
     CDS             complement(222..377)
                     /gene="hokD"
                     /locus_tag="ACUQPX_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HHV9058879.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(832..921)
                     /locus_tag="ACUQPX_004479"
     CDS             complement(832..921)
                     /locus_tag="ACUQPX_004479"
                     /inference="COORDINATES: protein motif:HMM:NF016343.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HHV9058880.1"
                     /translation="MAFVDKDGVEVESSTHWMPLPEPPQEVNQ"
BASE COUNT          226 a          252 c          217 g          273 t
ORIGIN      
        1 ttaatctgaa aaacatttat ttcacgccgt atatttgatt gtttaatgtg caagtacaat
       61 gacttttatt ttttgttgtg tatataatca aatatatggt tatttttcac cctgcgtatt
      121 cagcacgcaa caaaaaaccc gccgaagcgg gtttagtgcg ggtgcgttga ggatgcctga
      181 cacatgagag gtggcgaggg atttctcccc cgcttggtct cttactcctc aggttcgtaa
      241 gctgtgaaga cagcgacctc cgtctggccg gttcggattc gtacctcgca gaggtctttc
      301 ctcgttacca gtgccgtcac tatgacggtt aaacagatga cgatcagggc gattaacatc
      361 gccttttgct gcttcatagc ctgcttctcc ttgcctttcg gcacgtaaga ggctaaccta
      421 catttgtgag acatagattg ggcctcagat taatgttaag cgtcttgcag gacgcgtaat
      481 gttaactggg gcttttctct gtctgcctta cagtggcatg cccgaggcag acagcctcaa
      541 gcacccgcag caatcttacc gataccgata agaaaacgct attttttatt gccagcacct
      601 tctatccagg ctaatttatc tgcgtcagaa cggcgcaatg cgctcgcctg agatacgttt
      661 acttgccatg gtaacaacag gtaacggcaa ttgctgtttc tgttttgttt ccttcaaaaa
      721 ccccggacca tcaatccggg gtttttgttt gttaacccca acggcaaatc gaatacacca
      781 ccagcgccac cgccatcgca attcctaccg tggtgaatgc ctcaggccag gtcattgatt
      841 cacctcctgc ggtggttccg gtagcggcat ccagtgagtt gagctctcta cttcaacgcc
      901 gtctttgtcc acaaaagcca tcttattccc gccacgagtg gggcaaaaaa ccttatccca
      961 ggaaacgg
//
LOCUS       DBKEKI010000343          958 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.349, whole genome shotgun sequence.
ACCESSION   DBKEKI010000343 DBKEKI010000000
VERSION     DBKEKI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.349"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(65..>958)
                     /gene="solA"
                     /locus_tag="ACUQPX_004480"
     CDS             complement(65..>958)
                     /gene="solA"
                     /locus_tag="ACUQPX_004480"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HHV9058881.1"
                     /translation="RHNEDDPIFVRSGVINLGPADSAFLANVAHSAEQWQLNVEKLDA
                     QGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAKEAGCAQLFNCPVTAIRH
                     DDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRKVFAWYQADGRYSVKNKF
                     PAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERVPFAEVASDGSEAFPFLR
                     NVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITGLSGHGFKFASVLGEIAA
                     DFAQDQKSDFDLTPFRLSRFQ"
BASE COUNT          236 a          251 c          242 g          229 t
ORIGIN      
        1 tgcaacagcc gacgcattaa atactcctgt aaaaacggcc tcattctgga ggccgtaatg
       61 atgattattg gaagcgggaa agcctgaatg gcgtcaaatc aaaatcgctt ttttggtctt
      121 gcgcaaaatc ggcagctatt tcccctaaaa ctgacgcaaa tttaaaaccg tgcccactca
      181 ggccggtaat gagcagtgta ttatcgtggc cgggtagggt atcgataata aaatcttcgt
      241 caggcgaatt atcataggtg caggcagcgc cgtacaggca gcaaccgata cccggcaata
      301 cattgcgcaa gaacgggaag gcttctgacc catcactggc cacttccgca aacggaacac
      361 gttcatccgc tgaatggata acctggcctc cgttatgttt gccaatcttc aacgcgtcgt
      421 tttctgccgg aaaaccataa tattgatcgc cattgggcag ttcaccggta aacgccggga
      481 atttattctt cacgctatag cggccatcgg cctgatacca ggcaaatact ttacgtacgg
      541 gctggacagg cagctccggg agcaggtctt ttacccatgt tcccgcgcag acaatcgctt
      601 ttttcgcctg atactcaccg tcaaccgttt caatagttac gccatcatcg tcatgacgaa
      661 ttgcggtgac cgggcagttg aacagttgcg cacagcccgc ttccttcgcc agttggatcc
      721 aggttttaat cgccagttcg ctgcgcaaaa aaccggaatc agtctcaaat aagccgatgt
      781 agttgtccgg gacgcgtatt tctggccagc gggccataat cccttgcgca tcgagctttt
      841 caacgttgag ttgccactgt tcggcgctgt gggcgacgtt ggcgagaaat gcggagtcag
      901 ccgggccgag gttaatgaca ccagagcgta caaaaatggg atcatcttcg ttgtggcg
//
LOCUS       DBKEKI010000344          908 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.350, whole genome shotgun sequence.
ACCESSION   DBKEKI010000344 DBKEKI010000000
VERSION     DBKEKI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.350"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..84
                     /locus_tag="ACUQPX_004481"
     CDS             <1..84
                     /locus_tag="ACUQPX_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHV9058882.1"
                     /translation="SADNIAGVYYRPIQKYINGTWYNVASV"
     gene            86..613
                     /locus_tag="ACUQPX_004482"
     CDS             86..613
                     /locus_tag="ACUQPX_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HHV9058883.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWAEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEERARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(642..>908)
                     /locus_tag="ACUQPX_004483"
     CDS             complement(642..>908)
                     /locus_tag="ACUQPX_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HHV9058884.1"
                     /translation="ISGKWMFKDGIVIKRTYTGEEQRQQMENEKQSLLQLVRDKTQLW
                     DSQLRLGIISAGNKQKLTEWMLYAQKVESTDTSSLPVTFPEQPE"
BASE COUNT          254 a          195 c          223 g          236 t
ORIGIN      
        1 tctgccgata atattgcggg tgtttattac agaccgattc aaaagtatat aaatggcacc
       61 tggtataatg tagcgagcgt ttaatatgat gcacttaaag aacattaaag cgggtaacgc
      121 taaaacactg gaacagtacg aattaacaaa gaattttgac gtaatctggt tgtgggcaga
      181 agacggaaaa aactggtatg gggaagtgaa gaactttcag ccagatacaa taaagattgt
      241 ttacgatgaa aataatatta ttgtcgccat cactaaagat gcctccacgc ttaatcctga
      301 aggctttagc gtcgttgagg tccccgatat aacagcaaat cgtcgtgccg atgattccgg
      361 gaagtggatg tttaaggacg gagctgtggt taagcggatt tatacggcag acgaacagca
      421 gcaacaggcc gaatcacaaa aagccgcatt gctttccgaa gctgaatcag tcatccagcc
      481 gctggagcgc tctgtcaggc tgaatatggc aacagatgag gaacgcgcac gactggagtc
      541 atgggaacgc tacagtgttc tggtcagccg tgtggatacg gcaaagccag aatggccaca
      601 aaaaccagag taaaaattaa ggcccgatag cgggccttct ctcattctgg ttgttcggga
      661 aacgttactg gcagactgga ggtgtctgtg gattcgacct tctgcgcgta gagcatccac
      721 tcggttaatt tctgcttatt cccggcggaa atgatgccca gccgtagctg tgagtcccat
      781 agctgggttt tatccctgac aagttgcaac aggctttgct tttcattttc catttgttgc
      841 ctctgctctt ccccggtata agttcgcttt atcactatgc catctttgaa catccattta
      901 cctgaaat
//
LOCUS       DBKEKI010000345          890 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.352, whole genome shotgun sequence.
ACCESSION   DBKEKI010000345 DBKEKI010000000
VERSION     DBKEKI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.352"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..788)
                     /locus_tag="ACUQPX_004484"
     CDS             complement(<1..788)
                     /locus_tag="ACUQPX_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9058885.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENV"
BASE COUNT          250 a          203 c          188 g          249 t
ORIGIN      
        1 acattttctc ccgtagccag tatttttcca ttacgcatta gagtgacacc cactcccgat
       61 gcttttgttg catcaggtgc tgtgttagca aacacttgtc ggctactgtc agtggtggca
      121 cccgaaagat aaaatgagag cttttgttcg cttgagcaat atacgccaag gggtatttct
      181 gcgcttccag gaaagtccgg gagatccacg gtgacattac gactatcaac ggtgcagccg
      241 cctgttggca taacaacact gttatttgaa ataatattcc acgtaaaatt acgcggattt
      301 ccgcttccta atgttgcaat tttatacata tgaatacggg caatgacttc ccccgctttt
      361 atgacaactc caccggccgc cccaactggg gtaatataga gttttaacgg taatggcatc
      421 ggtgtcttat caccaatatc caatacgttg gtatttgtcg tcaacgggaa ggggtaggtc
      481 acattattcc agtacagcga tcctttatat gactgtaggg agcctgcgaa agctgagcct
      541 tgtaccaggt ttatatgatc agtgtcgtac cagccgccgt aatcattcca gcaacttata
      601 tgctgagaca agtctacaac cagattctgg cccggctgta ttacagggtc gagattaaca
      661 taaacagagg ttgtacctgc tccaatactt gacccgccat cgacattaca actgaaggca
      721 aaaactttgc ctgccattaa tagaagataa ataccgaaca ggacctttat actgattgtt
      781 ttacccatga tatatcctaa ggttaaaaat tgatttaaaa agaaggctaa gaaaatcgaa
      841 tcgacgttat tgccaggtgt agatcacatt gattagcgcc tcgatcgttc
//
LOCUS       DBKEKI010000346          873 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.353, whole genome shotgun sequence.
ACCESSION   DBKEKI010000346 DBKEKI010000000
VERSION     DBKEKI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.353"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..342)
                     /locus_tag="ACUQPX_004485"
     CDS             complement(<1..342)
                     /locus_tag="ACUQPX_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HHV9058886.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNHVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYS"
     gene            complement(342..>873)
                     /gene="clsB"
                     /locus_tag="ACUQPX_004486"
     CDS             complement(342..>873)
                     /gene="clsB"
                     /locus_tag="ACUQPX_004486"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HHV9058887.1"
                     /translation="HALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVKGGVQV
                     FEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRDNLNGI
                     IAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDPPAQPT
                     METQDRVETENTGVKP"
BASE COUNT          205 a          245 c          234 g          189 t
ORIGIN      
        1 ggagtacaaa cgatagcgca taccaatgcc gccaacccag gtactgagcg tcaggttgaa
       61 ggcgtagcag ataaacgaca ccagcatcac ctggcgcttc gccagtttgt gaccgcagta
      121 aaagcgggcg agcaggtcat agcagccgta aatcagatag ctgacgacca ccagcccgac
      181 cgcactaagc agcgcaacgt gattgtagtc gcggatgacc ttccagacct cttcccagtc
      241 cacttttttg gcgtagacca ccagcaacac aatcaccgcg ataaaaaaca gccaggtgag
      301 gatcttcttt gctaagcgcc agcgcgggtg tgatttactc atcagggttt tacccccgtg
      361 ttttcagttt ctacccgatc ctgcgtttcc attgtcggtt gtgcgggggg atcaacctgc
      421 gtcagacgtg gtgtgtgtgc cggaagccag ccaaccagcg ccgggaagtg gcgtaaaaag
      481 tggaacgcca gcacgctttt ggtcaggttc caccaggtgc gtttgggcag catggtttca
      541 tccacctgct gacaatctgc ggcaataatg ccgttcagat tatcgcgcag cgtctggtta
      601 aaatgacgat cgtggatgat gacatttgct tcgagattca gtgacaaact gagcggatcg
      661 agattactgg accctactgt cgcccagtga tcgtccatca atgccacttt gccgtggagc
      721 gggcggcggc ggtactcaaa aacctgaacg ccgcctttaa ccagatagtt atacagcaag
      781 cgcgcaccga ctctgacaat cggcatatcc ggttcgccct gaatgatcag tttgatccgc
      841 accccgcgcc gtgccgcttt acgcaaggcg tgt
//
LOCUS       DBKEKI010000347          865 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.355, whole genome shotgun sequence.
ACCESSION   DBKEKI010000347 DBKEKI010000000
VERSION     DBKEKI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.355"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            105..>865
                     /gene="ltrA"
                     /locus_tag="ACUQPX_004487"
     CDS             105..>865
                     /gene="ltrA"
                     /locus_tag="ACUQPX_004487"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HHV9058888.1"
                     /translation="MPHCADGAAATTGDNADGRTAVKSAKPFPVSKRQVWEAYKRVKA
                     NRGAAGIDGQTLAGFDENVTDNLYKLWNRMASGSYMPQAVRRVDIPKADGGVRPLGIP
                     AVSDRIAQMVVKQILEPVLEPLFHADSYGYRPGKSAHQAIAQARKRCWKFDWVVEVDI
                     KGFFDDIDHHLLLKTVQHHTQARWVVMYIERRLKAPVQMPDGAMLARGRGTPQGGVIS
                     PLLSNLFLHYAFDMWMQRQFPGVPFERYADDVVCH"
BASE COUNT          210 a          206 c          268 g          181 t
ORIGIN      
        1 acaacgctcc gatggaaagc ttctggggaa cgctgaaaaa tggtacaggg acggagtaaa
       61 ccgtagtagc ggtgaaggac cgtaatgggt ccggagcaaa gggcttgccc cattgtgctg
      121 atggtgcggc ggcaaccacg ggcgacaatg ccgatgggag gactgctgta aaatcggcaa
      181 agccatttcc tgtaagtaaa cgacaggtat gggaagctta taaacgagta aaagccaacc
      241 gtggggcggc aggcattgac gggcagacac tggccgggtt tgacgagaat gttacagaca
      301 atctctacaa actgtggaac agaatggctt ccggcagtta tatgccgcag gcggtaaggc
      361 gtgtggacat tcccaaagct gacggtggtg tacgtccgct cggtattccg gccgtgtcgg
      421 accggatcgc ccagatggtc gttaagcaga tactcgagcc ggtactggaa cccctgttcc
      481 atgcagactc gtatggttat cgaccgggta aatccgcaca ccaagcgatt gcgcaggcga
      541 ggaaacgctg ctggaagttt gactgggtgg tggaagtcga tattaaaggc ttctttgatg
      601 atatcgatca tcacctgcta ctgaagacag tgcagcatca tactcaggca cgctgggtgg
      661 tgatgtacat tgagcgcagg ctgaaggcac cggtgcaaat gccggatgga gcaatgctgg
      721 cacggggtcg aggaacgccg cagggcggcg taatctcccc cctgctctca aatctgttcc
      781 tgcactatgc ctttgatatg tggatgcaac gtcagtttcc cggcgttccc ttcgaaaggt
      841 acgccgatga tgttgtatgc cactg
//
LOCUS       DBKEKI010000348          859 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.356, whole genome shotgun sequence.
ACCESSION   DBKEKI010000348 DBKEKI010000000
VERSION     DBKEKI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.356"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..296)
                     /locus_tag="ACUQPX_004488"
     CDS             complement(<1..296)
                     /locus_tag="ACUQPX_004488"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HHV9058889.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDA"
     gene            complement(397..657)
                     /gene="iraP"
                     /locus_tag="ACUQPX_004489"
     CDS             complement(397..657)
                     /gene="iraP"
                     /locus_tag="ACUQPX_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HHV9058890.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          243 a          197 c          160 g          259 t
ORIGIN      
        1 gcatcgatac ctttaaaaaa gccgcccgca ccttcggcat aattacgtgc ggcagtaatt
       61 tccgaatccc ccatcccatc gccaatcagc aaaataatat tttttgcagg tttatcgcta
      121 agagaatcac gcagagcggc ggtctgatca cccgttaagc ggcgagcacc gccgggtgca
      181 gtaatatcgc cctgagcagc ccggttttcc agaacaggca tttctggtgt ccgggctttt
      241 gtcacagggg taaacagtaa cggtaagagt gccagtgcaa tagtgctttg tttcacttta
      301 ttttctccat gtacaaatac attaaaaaat aaaaacaaag cgactataag tctcggccgt
      361 gacaacttta tgacagctgt tgaaaagatt aactttttac tgacgaggat gcttcaataa
      421 cttctttacg taatcgcgca gcagctccgt atcgtcgtca ggaatgctgg catcgggctt
      481 tacctcgtac agcgccccct ctacctgatc aatcaaccgc tgttggtcat tttgcgccat
      541 attgcgaagc attgcagtga cgataatctc caaagcttct acctgcgcac acagttcttt
      601 cgactcttct tctttttggg caagcttaaa taacaactca gcaatgagat ttttcatgtc
      661 tgtatttcct tatccaaagt atggagaagt taacattatg tttcacaact tcatagtgtc
      721 tggaagtatt atttttatga caaaggaaat actttgcgca aatatttttt gtttgattac
      781 cagcgaaacg tttctcttat ttaaataatt acccacacaa aatataggct tttaattatc
      841 aacctttatt ataaataaa
//
LOCUS       DBKEKI010000349          851 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.357, whole genome shotgun sequence.
ACCESSION   DBKEKI010000349 DBKEKI010000000
VERSION     DBKEKI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.357"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            141..>851
                     /gene="fliC"
                     /locus_tag="ACUQPX_004490"
     CDS             141..>851
                     /gene="fliC"
                     /locus_tag="ACUQPX_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HHV9058891.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGT"
BASE COUNT          239 a          189 c          217 g          206 t
ORIGIN      
        1 tcgactaaca aaaaatggct gtttgtgaaa aaaattctaa aggttgtttt acgacagacg
       61 ataacagggt tgacggcgat tgagccgacg ggtggaaacc caaaacgtaa tcaacgactt
      121 gcaatatagg ataacgaatc atggcacaag tcattaatac caacagcctc tcgctgatca
      181 ctcaaaataa tatcaacaag aaccagtctg cgctgtcgag ttctatcgag cgtctgtctt
      241 ctggcttgcg tattaacagc gcgaaggatg acgccgcggg tcaggcgatt gctaaccgtt
      301 ttacttctaa cattaaaggc ctgactcagg ctgcacgtaa cgccaacgac ggtatttctg
      361 ttgcacagac cactgaaggc gcgctgtccg aaatcaacaa caacttacag cgtatccgtg
      421 agctgacggt tcaggcttct accgggacta actctgattc ggatctggac tccattcagg
      481 acgaaatcaa atcccgtctc gacgaaattg accgcgtatc cggtcagacc cagttcaacg
      541 gcgtgaacgt actggcaaaa gacggttcga tgaaaattca ggttggtgcg aatgacggcc
      601 agactatcac tattgatctg aagaaaattg actctgatac gctggggctg aatgggttta
      661 atgtgaatgg tggcggggct gtggctaata ctgctgcatc taaagctgac ttggtagctg
      721 ctaatgcaac agtggtcggc aacaaatata ctgtgagtgc gggttacgat gctgctaaag
      781 cgtctgattt gctggctgga gttagtgatg gtgatactgt tcaggcaacc attaataacg
      841 gcttcggaac g
//
LOCUS       DBKEKI010000350          844 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.358, whole genome shotgun sequence.
ACCESSION   DBKEKI010000350 DBKEKI010000000
VERSION     DBKEKI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.358"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..487
                     /locus_tag="ACUQPX_004491"
     CDS             <1..487
                     /locus_tag="ACUQPX_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HHV9058892.1"
                     /translation="DSKWIPSHHISSNEVKKTENEVRQTEDKQHQNSEPEEGTPCNQT
                     GEDNCPDCGAVMGDATYQETFDEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNN
                     ETGEDELNWKKQVLIAAVYGLCANPACIAYAPAIPDIAMMIANKLENFGVTSDERLPD
                     F"
     gene            465..761
                     /locus_tag="ACUQPX_004492"
     CDS             465..761
                     /locus_tag="ACUQPX_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HHV9058893.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
BASE COUNT          273 a          181 c          216 g          174 t
ORIGIN      
        1 tgattcgaaa tggattccca gtcatcatat ctcctcaaat gaagtcaaaa aaacggaaaa
       61 tgaagtgcga cagactgaag ataaacagca ccagaacagc gaaccggagg agggaacacc
      121 ctgcaatcag actggcgagg ataactgccc tgactgtggc gcggtgatgg gagacgcaac
      181 ttatcaggaa acttttgacg aaaaaaaacc agatgaagca caggaagaag agccgaagaa
      241 aacggaaaaa gctgatgatt tgttaccgga gaacgctggc agcgatcagc ataatgatag
      301 caataatgaa actggcgaag atgaactcaa ctggaaaaaa caggtgttga ttgcggcggt
      361 ttacggttta tgtgcgaatc ctgcgtgcat agcctatgcg cctgcaatcc ctgacatcgc
      421 aatgatgatt gccaacaagc ttgaaaattt tggagtgacc agtgatgagc gcttacccga
      481 tttttgatcg tattgaagaa atggcatggg ctcgccacta ccagcagatc gttcgtgaag
      541 aaaaagaaac ggaactggcg gacgacctgg aaaaaggtct gcctcagcac ctgtttgaat
      601 cgctctgcat cgaccatttg caacgccacg gtgccaataa acaggcaatc agccacgcat
      661 ttgatgatga tgttgagttt caggagcgca tggcagaaca catccggtac atggctgaaa
      721 ccattgccca ccaccaggtt gatattgatt tggaggacta aaatctatgg cgctttttca
      781 acgagcaata aacacacagg cctatctaaa agcaggaatt atggggtttg ctggcgacgg
      841 caaa
//
LOCUS       DBKEKI010000351          828 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.359, whole genome shotgun sequence.
ACCESSION   DBKEKI010000351 DBKEKI010000000
VERSION     DBKEKI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.359"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>828
                     /gene="ompN"
                     /locus_tag="ACUQPX_004493"
     CDS             <1..>828
                     /gene="ompN"
                     /locus_tag="ACUQPX_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HHV9058894.1"
                     /translation="LLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYFSDNSAKD
                     GDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGLKFADYGS
                     FDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFFGLVNGLN
                     FAVQYQGNNEGASNGQEGTNNERDVRHENGDGWGLSTTYDLGMGFSAGAAYTSSDRTN
                     DQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYVVANKTQNFEV
                     "
BASE COUNT          236 a          182 c          202 g          208 t
ORIGIN      
        1 acttttaatt cctgccctgc tcgccgcagg tgctgcacat gcagccgaag tttataataa
       61 agacggcaac aaattagatc tgtatggcaa agttgatggc ctgcattatt tttctgataa
      121 ttcagcgaaa gatggcgacc agagctatgc gcgtctgggt tttaaaggcg aaacccaaat
      181 taacgatcaa cttactggct acggtcaatg ggaatacaat attcaggcaa acaacactga
      241 atcttcaaaa aaccagtcat ggacccgtct ggcatttgcc ggactgaaat ttgcagatta
      301 cggttctttc gattacggac gtaattatgg cgtaatgtac gacatcgaag gctggaccga
      361 tatgctgcct gaatttggcg gtgactctta taccaatgca gacaacttta tgactggtcg
      421 agccaatggc gtcgcgactt atcgtaatac tgatttcttc ggtctggtaa atggtctgaa
      481 cttcgcggtg cagtatcaag gtaacaacga aggagccagt aatggtcagg aaggcaccaa
      541 taacgaacgc gatgttcgtc atgaaaacgg tgacggctgg ggtctttcca caacatatga
      601 tttaggcatg ggctttagcg ctggtgcggc atacacctct tctgaccgca ccaatgacca
      661 ggttaaccat actgcggcgg gtggtgataa agcagacgcg tggactgctg ggctaaaata
      721 cgatgctaac aatatttacc tagcaaccat gtattcagaa acgcgtaata tgaccccgtt
      781 tggcgacagc gattatgtag ttgcgaacaa aacccagaac tttgaagt
//
LOCUS       DBKEKI010000352          827 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.360, whole genome shotgun sequence.
ACCESSION   DBKEKI010000352 DBKEKI010000000
VERSION     DBKEKI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.360"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(12..827)
                     /gene="sul2"
                     /locus_tag="ACUQPX_004494"
     CDS             complement(12..827)
                     /gene="sul2"
                     /locus_tag="ACUQPX_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HHV9058895.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          181 a          249 c          253 g          144 t
ORIGIN      
        1 cgaatgtgca gttaacgaat tcttgcggtt tctttcagcg ccgccaatac cgccagcccg
       61 tcgcgcaagg ggcgcggctc gtgtgtgcgg atgaagtcag ctccacctgc ggcggcggca
      121 agctctgcag cgagtgtcgc ggccccgaca tcccccggac cacggcctgt gagcgcgcgc
      181 agaaaggatt tgcgcgaaac agacagaagc accggcaaat cgaagcgcag ccgcaattca
      241 tcgaaccgcg ccagcaccga gagcgaggtt tcgggagcag cccccagaaa aaaccccatg
      301 ccgggatcaa ggacaaggcg gttgcgtttg ataccggcac ccgtcagcgc cgcgatgcgc
      361 gcgtcaaaga acgccgcaat gtgatccatg atgtcgccag cgggtgcctc gcgccgatct
      421 gcctgcccgt cttgcaccga atgcataacg acgagtttgg cagatgattt cgccaattgc
      481 ggatagaacg cagcgtctgg aaaaccgcga atatcattga gataggccac accacgcgac
      541 aaggcatagg cttgcgtcgc gggttgataa ctgtcgagcg agacgggaat gccatctgcc
      601 ttgagcgcgt ccagcaccgg cgcgatacgc gcgatttctg tgtcggacga aacaggcgcg
      661 gcgtcgggat tgctggatgc cggaccgagg tcgatcacat ctgccccctc ggccatcagc
      721 ttacgcgcct gcgcaatggc tgcgtctggc gccagatacc ggcctccatc ggagaaactg
      781 tccgaggtta tgttgacgat gccgaaaatg atgagcgatt tattcat
//
LOCUS       DBKEKI010000353          814 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.361, whole genome shotgun sequence.
ACCESSION   DBKEKI010000353 DBKEKI010000000
VERSION     DBKEKI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.361"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            170..766
                     /gene="fimE"
                     /locus_tag="ACUQPX_004495"
     CDS             170..766
                     /gene="fimE"
                     /locus_tag="ACUQPX_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HHV9058896.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          223 a          152 c          192 g          247 t
ORIGIN      
        1 atgttgtcac atcagtgata ttttattttt gtatgatgtt taatgtaatt gactgatagc
       61 cacataactt cgtgtgtggt tatcttttta tctattgggc taattttgac cgattgaggt
      121 ttcctatagg tattcattca aatatatctc agttaggagt actactattg tgagtaaacg
      181 tcgttatctt accggtaaag aagttcaggc catgatgcag gcggtttgtt acggggcaac
      241 gggagccaga gattattgtc ttattctgtt ggcatatcgg catgggatgc gtattagtga
      301 actgcttgat ctgcattatc aggaccttga ccttaatgaa ggtcgaataa atattcgccg
      361 actgaagaac ggattttcta ccgttcaccc gttacgtttt gatgagcgtg aagccgtgga
      421 acgctggacc caggaacgtg ctaactggaa aggcgctgac cggaccgacg ctatatttat
      481 ttctcgccgc gggagtcggc tttctcgcca gcaggcctat cgcattattc gcgatgccgg
      541 tattgaagct ggaaccgtaa cgcagactca tcctcatatg ttaaggcatg cttgcggtta
      601 tgaactggcg gagcgtggtg cagatacccg tttaattcag gattatctcg ggcatcgaaa
      661 tattcgccat actgtgcgtt ataccgccag taatgctgct cgttttgccg gattatggga
      721 aagaaataat ctcataaacg aaaaattaaa aagagaagag gtttgattta acttattgat
      781 aataaagtta aaaaacaaat aaacacaaga caat
//
LOCUS       DBKEKI010000354          814 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.362, whole genome shotgun sequence.
ACCESSION   DBKEKI010000354 DBKEKI010000000
VERSION     DBKEKI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.362"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..737
                     /gene="yeeO"
                     /locus_tag="ACUQPX_004496"
     CDS             <1..737
                     /gene="yeeO"
                     /locus_tag="ACUQPX_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HHV9058897.1"
                     /translation="ALRISLKSYFKPLNFSIIWEVMGIGIPASVESVLFTSGRLLTQM
                     FVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVGQIAQAEIQLRHVF
                     WLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALFMPIWSASWVLPAG
                     FKGARDARYAMWVSMLSMWGCRVVVGYVQGIMLGWGVVGVWMGMFADWAVRAVLFYWR
                     MVTGRWLWKYPRSEPQKCEKKPVVSE"
BASE COUNT          172 a          162 c          237 g          243 t
ORIGIN      
        1 ctgcgctaag gatttcgtta aagagctatt ttaaaccgct gaattttagc attatctggg
       61 aagtcatggg gattggtatt ccagcgagtg tcgaatcagt gttatttacc agtggtcggt
      121 tattaaccca aatgttcgtt gccgggatgg ggaccagcgt tattgccgga aattttatcg
      181 cgttctcaat tgcggctctt atcaacttac ccggaagtgc gctcggttct gcttctacga
      241 tcattacagg ccgaaggttg ggggtagggc agatagcgca agcagagatt cagttgcggc
      301 atgtgttctg gctgtccact cttggattaa cggccatcgc ctggctaacg gctccctttg
      361 ccggggttat ggcatcgttt tacacccagg atccacaggt taaacacgtc gttgtgattc
      421 tgatttggct aaatgcttta tttatgccta tttggtccgc ctcatgggtg ctacccgctg
      481 gatttaaggg tgctcgtgat gcccgttacg ccatgtgggt ttctatgttg agcatgtggg
      541 gttgccgggt tgtagtcggt tatgtgcagg gaatcatgct tggctggggt gtggttggtg
      601 tctggatggg aatgtttgcc gactgggctg tgcgggccgt gctgttttac tggcgaatgg
      661 ttactggacg ttggctgtgg aaataccctc gatccgagcc gcaaaagtgt gaaaaaaagc
      721 cagttgtgtc ggaataaacg acaaaatgca gattatttca gcaaaagatt tcaaatttaa
      781 aaaacaggct ttgacattgt gggtgggcat cgct
//
LOCUS       DBKEKI010000355          811 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.363, whole genome shotgun sequence.
ACCESSION   DBKEKI010000355 DBKEKI010000000
VERSION     DBKEKI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.363"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(177..338)
                     /locus_tag="ACUQPX_004497"
     CDS             complement(177..338)
                     /locus_tag="ACUQPX_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HHV9058898.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
BASE COUNT          197 a          212 c          214 g          188 t
ORIGIN      
        1 tggaacagga caattaacaa cagatctact tgagatcatt tgcttgtcgt cgtagtctct
       61 ttcccagaaa cgaaaaaacc gccctgcaag gcggtttctc gaaggttctc agagcaacca
      121 actctttgaa ccaaggtaac tggcttatga ggagcgcagt aaccaaattt gtcctttcag
      181 tttagcctta acaggcgcat aacttcaaga ctaactcctc taaatccgtt accaatggct
      241 accgccactg gcgatttgtc gtgtctttcc gggttggact caagttgata gttaccggaa
      301 cgggcgtggt agtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact
      361 gcctacccgg aactgagtgt caggcgtgga atgagataaa cgcggccata acagcggaat
      421 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccac cagggggaaa
      481 cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagattct
      541 gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc agcgccgcat cctttttctg
      601 ccccgcttgt catgccgtat gcctttgcac ttatccgcga gcaggtaacg cccgccgcag
      661 tcgaacggca ggagcggcag cgaccgagtg agcgaggagg cgtcatatca ccgtaacctc
      721 cattttctcc tgtacgccct gcagctgcat tttcccctgt cacatgaagc actttcatta
      781 agtgttactc cgctgtaaca tattaagcca g
//
LOCUS       DBKEKI010000356          808 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.364, whole genome shotgun sequence.
ACCESSION   DBKEKI010000356 DBKEKI010000000
VERSION     DBKEKI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.364"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..135)
                     /locus_tag="ACUQPX_004498"
     CDS             complement(<1..135)
                     /locus_tag="ACUQPX_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HHV9058899.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     P"
     gene            complement(135..734)
                     /locus_tag="ACUQPX_004499"
     CDS             complement(135..734)
                     /locus_tag="ACUQPX_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HHV9058900.1"
                     /translation="MAHIQLVKQTSSGLLLPATPENCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          198 a          212 c          192 g          206 t
ORIGIN      
        1 tggttttgtc gctgttccgc acgtccccgc cagcctgtaa tgcgccagca cagaagtttc
       61 gggattgtga ttgcagtagc caggaattct gacggtgcac atctgccccc gcgccgcttt
      121 acgtaaatcc accattacgc aaactccagt aactgcgcgg ccacattttc gacttcctcc
      181 ggagaggaaa atttacggaa caggatccag ttccacagca cattcagtac agatttataa
      241 acctgctgaa actcggtttc gtccatgttc gcaaatgcga tagattttgc cctgcgccca
      301 cgactaccat caggataaat atgctcggtg taaaatccgg cctgaatggt tacccactcg
      361 cggaaagcgt caaacgactt tagcaatgcc gtatcccggg ttctgcatgt cgcaactgta
      421 ttaaggtatt gctctgcggc atcactcagg gctggagtgt gttcccgacc tactgattcg
      481 cacaggtaat caacgaagcc tgataccagt tttcgttcgc gaggcgtgat cgccccaccg
      541 accggagtcc agtaatcaaa tcccagttgc aggagtttga aaaaacgctt gtggaatgcg
      601 tagttacgca cacgcttaaa gtctgcgtgt atccactcac ctattttgat ttgatgcaaa
      661 aaatcgcaat tctccggcgt cgccgggaga agtaatccgg aagaggtttg tttgaccagt
      721 tgtatatgcg ccatcgtagt tctccgctgg cgcagtagaa tgggtgttca gcccgttatg
      781 tagtatacca gaattaatgc caatacta
//
LOCUS       DBKEKI010000357          799 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.365, whole genome shotgun sequence.
ACCESSION   DBKEKI010000357 DBKEKI010000000
VERSION     DBKEKI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.365"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..427
                     /gene="otsB"
                     /locus_tag="ACUQPX_004500"
     CDS             <1..427
                     /gene="otsB"
                     /locus_tag="ACUQPX_004500"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HHV9058901.1"
                     /translation="GMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQQGKCVVEIK
                     PRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVKIGTGATQAS
                     WRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            402..799
                     /locus_tag="ACUQPX_004501"
                     /pseudo
     CDS             402..799
                     /locus_tag="ACUQPX_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
BASE COUNT          210 a          182 c          220 g          187 t
ORIGIN      
        1 agggatggct tttgcgctgc attatcgtca ggctccgcag catgaagacg cattaatgac
       61 attagcgcaa cgtattactc agatctggcc acaaatggcg ttacagcagg gaaagtgtgt
      121 tgtcgagatc aaaccgagag gtaccagtaa aggtgaagcg attgcagcct ttatgcagga
      181 agctcctttt atcgggcgaa cgcccgtatt tctgggcgat gatttaaccg atgaatctgg
      241 cttcgcagtc gttaaccgac tgggcggaat gtcagtaaaa attggcacag gtgcaactca
      301 ggcatcatgg cgactggcgg gtgtgccgga tgtctggagc tggcttgaaa tgataaccac
      361 cgcattacaa caaaaaagag aaaataacag gagtgatgac tatgagtcgt ttagtcgtag
      421 tatctaaccg gattgcacca ccagacgagc acgccgccag tgccggtggc cttgccgttg
      481 gcatactggg ggcactgaaa gccgcaggcg gactgtggtt tggctggagt ggtgaaacag
      541 ggaatgagga tcagccgcta aaaaaggtga aaaaaggtaa cattacgtgg gcctctttta
      601 actcagcgaa caggaccttg acgaatacta caaccaattc tccaatgccg ttctctggcc
      661 cgcttttcat tatcggctcg atctggtgca atttcagcgt cctgcctggg acggctatct
      721 acgcgtaaat gcgttgctgg cagataaatt actgccgctg ttgcaagacg atgacattat
      781 ctggatccac gattatcac
//
LOCUS       DBKEKI010000358          780 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.366, whole genome shotgun sequence.
ACCESSION   DBKEKI010000358 DBKEKI010000000
VERSION     DBKEKI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.366"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            67..>780
                     /locus_tag="ACUQPX_004502"
     CDS             67..>780
                     /locus_tag="ACUQPX_004502"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHV9058902.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAE"
BASE COUNT          225 a          189 c          183 g          183 t
ORIGIN      
        1 aatgatcaat atctatagtg atatcaccaa ccgtcaaaac gaaccgacaa ctccatgagg
       61 aactgaatga tgaataataa agtcagcttc actaacagca ataatccaac catctctttg
      121 tctgcagtga tctatttccc tccaaaattt gatgaaaccc gtcagtatcc ggcaattgtg
      181 gtatctcacc caggtggggg cgttaaagag caaacggccg gaacctatgc cgaaaagctg
      241 gcggaaaagg gatttgtcac tattgcttat gacgcatctt atcaaggtga aagtggcggc
      301 gagccgcgtc agttagaaaa cccatacatt cgtactgaag acgtcagtgc cgtgattgat
      361 tacctgacaa cgctttctta tgtcgacaat acccgaattg gtgcgatggg aatttgtgcc
      421 ggtgcaggat atacagccaa tgctgcaatt caggatcgcc gcatcaaggc catcggtacc
      481 gttagtgcgg tcaatattgg ttcgatgttc cgcaatggtt gggaaaacaa tgtgaaatct
      541 attgatgctc tgccacacat tgaagctggc tcaaacgccc gaaccagcga tattagcagc
      601 gaagaatacg ccattatgcc actggctcca atgaaggagg ccgatgcgcc gaacgaagaa
      661 ctgcgtcagg cctgggagta ttaccacaca cctcgtgcgc agtatccaac agcaccgggt
      721 tacgctactt tgcgcagcct taaccagatt attacctatg atgcttacca tatggcggaa
//
LOCUS       DBKEKI010000359          777 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.367, whole genome shotgun sequence.
ACCESSION   DBKEKI010000359 DBKEKI010000000
VERSION     DBKEKI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.367"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>777
                     /gene="tdcB"
                     /locus_tag="ACUQPX_004503"
     CDS             <1..>777
                     /gene="tdcB"
                     /locus_tag="ACUQPX_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HHV9058903.1"
                     /translation="KFENMQRTGSFKIRGAFNKLSLLIDAEKRKGVVACSAGNHAQGV
                     SLSCAMLGIDGKVVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRI
                     FIPPYDDPKVIAGQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVI
                     GVQSENVHGMAASFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSED
                     EIRNSMIALIQRNKVVTEGAGALACAALLSGKLDQYIQNRK"
BASE COUNT          215 a          161 c          202 g          199 t
ORIGIN      
        1 aagtttgaaa atatgcagcg tacgggttca tttaaaattc gtggcgcatt taataaatta
       61 agtttactga tcgatgcgga aaaacgcaaa ggcgtggtgg cctgttctgc gggcaaccat
      121 gcgcaagggg tttcgctctc ctgcgcgatg ctgggtatcg acggtaaagt ggtgatgcca
      181 aaaggtgcgc caaaatccaa agtagcggca acgtgcgact actccgcaga agtcgttctg
      241 catggtgata acttcaacga cactatcgct aaagtgagcg aaattgtcga aatggaaggt
      301 cgtattttta tcccacctta cgatgatccg aaagtgattg ctggccaggg aacgattggt
      361 ctggaaatta tggaagatct ctatgatgtc gataacgtga ttgtgccaat tggtggtggt
      421 ggtttaattg ctggtattgc ggtggcaatt aaatctatta acccgaccat tcgtgttatt
      481 ggcgtacagt cagaaaacgt tcacggcatg gcggcttctt tccactccgg agaaataacc
      541 acgcaccgaa ctaccggcac cctggcggat ggttgtgatg tctcccgccc gggtaattta
      601 acttacgaaa tcgttcgtga attagtcgat gacatcgtgc tggtcagcga agacgaaatc
      661 agaaacagta tgattgcctt aattcagcgc aataaagtcg tcaccgaagg cgcaggcgct
      721 ctggcatgtg ctgcattatt aagcggtaaa ttagaccaat atattcaaaa cagaaaa
//
LOCUS       DBKEKI010000360          772 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.368, whole genome shotgun sequence.
ACCESSION   DBKEKI010000360 DBKEKI010000000
VERSION     DBKEKI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.368"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>772
                     /locus_tag="ACUQPX_004504"
     CDS             <1..>772
                     /locus_tag="ACUQPX_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HHV9058904.1"
                     /translation="IDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYPSQ
                     EHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTDAG
                     FQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLVKE
                     RKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRARE
                     IIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRIL"
BASE COUNT          230 a          157 c          186 g          199 t
ORIGIN      
        1 gtattgatcg cctcatgggt aatgaggcgc tccatcgcga tattccaatg attttcagga
       61 atatcacttc aatgctgaca cggcagttat ctttatgtgt aatagccgta gactggagtg
      121 gctatccctc ccaggagcat catgttcttc gtgccagcct tctgtgtgac gggcgttcca
      181 ttccgttatt gagtaaggtt gttccttctg aaaaacaaaa taatccattg atacaacatg
      241 atttccttga ttcccttgct caatccctgc cccccgatgc cagggttatt attgtcacgg
      301 atgctggatt tcagagcgca tggtttcacc atattacttc tctgggctgg gattttattg
      361 gtcggatcag aaataacgtt cagtattgcc tcgataacgc gcctgaaaga tggttaaaag
      421 tgtcggatag ccctgaatgt aaaacaccgg aatatatggg ggctggaagg ctggttaaag
      481 aaaggaaaaa aagcatccgg gggcattttt acacctataa gaaatcggca aaagggcgaa
      541 aaaagaaacg ttcgaaaggt cagagtggtt taaacaaaac agacaaagaa cagagcaaat
      601 cagcaaaaga agcctggttg atcttcagca gtacaaatga cttcagagcg cgagaaatca
      661 tcaagttata cagtcggcga atgcagattg agcaaaactt tcgtgatgaa aagaacgggc
      721 gcttcggatt tggtcttcgg gccagcaaaa gtcgttcaac aggaagaatt ct
//
LOCUS       DBKEKI010000361          747 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.369, whole genome shotgun sequence.
ACCESSION   DBKEKI010000361 DBKEKI010000000
VERSION     DBKEKI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.369"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..342
                     /locus_tag="ACUQPX_004505"
     CDS             <1..342
                     /locus_tag="ACUQPX_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HHV9058905.1"
                     /translation="AKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRR
                     GIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIAR
                     VRSVEEILHAL"
     gene            339..434
                     /locus_tag="ACUQPX_004506"
                     /pseudo
     CDS             339..434
                     /locus_tag="ACUQPX_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          218 a          176 c          165 g          188 t
ORIGIN      
        1 gctaaagtgt tgcccgaaaa ctggtggcaa catccagctg cattaggtgc aactgacagt
       61 gatatcgaaa tcatcaaacg tcagtggggt gcgttttacg gtacggatct ggagttgcaa
      121 ctgcgccgcc gaggtatcga tacaatagtg ttatgtggga tctcgaccaa tatcggtgtt
      181 gaatccaccg cccgcaatgc ctgggaactt ggctttaatc tggtgattgc cgaagacgcc
      241 tgtagtgccg ccagcgccga gcaacacaat aacagcatta atcatatcta cccgcgcatc
      301 gcccgtgtgc gtagcgtgga agagatcctt cacgcgttat gatttacatc ggtctaccgc
      361 aatggtcgca tcctaaatgg gtgcggttgg ggatcaccag ccttgaagag tatgcccgcc
      421 actttaactg cgtgatacgg gatttttaaa aatcactaaa gaacgcccaa gagcatgtgt
      481 tttctttaga ataatcaaag cgttggattg ccatgtcata tgaaaatcgg caaagttcat
      541 atgtaacaaa gtgtcccaat catgccccaa aatgccccac aacaaatatt tttgccccat
      601 gcatgcccca aaaaatcact ttccctcact cttgacactg tttatccata cagttaaaaa
      661 taatactgta taaaaacaca gtgtagaggg tttttatgcg tattgaaatc tgcatagcca
      721 aagagaaaat gactaaaatg ccaaccg
//
LOCUS       DBKEKI010000362          746 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.370, whole genome shotgun sequence.
ACCESSION   DBKEKI010000362 DBKEKI010000000
VERSION     DBKEKI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.370"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..341
                     /gene="dgcP"
                     /locus_tag="ACUQPX_004507"
     CDS             <1..341
                     /gene="dgcP"
                     /locus_tag="ACUQPX_004507"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HHV9058906.1"
                     /translation="LFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLR
                     ERIQQQIRGEYHLGDVDLYYPSASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKT
                     PFVAHPALHS"
     gene            374..472
                     /locus_tag="ACUQPX_004508"
     CDS             374..472
                     /locus_tag="ACUQPX_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HHV9058907.1"
                     /translation="MRIGIIFPVVIFITAVVFLACFFIGGYAAPGA"
     gene            475..549
                     /gene="yoaJ"
                     /locus_tag="ACUQPX_004509"
     CDS             475..549
                     /gene="yoaJ"
                     /locus_tag="ACUQPX_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HHV9058908.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT          193 a          155 c          180 g          218 t
ORIGIN      
        1 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
       61 ttggtcgtct cggtggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
      121 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
      181 ttgatttgta ttatcccagt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
      241 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
      301 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
      361 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttca ttacagcggt
      421 cgtattttta gcatgttttt ttattggcgg ctatgctgcc ccgggagcat aaagatgaaa
      481 aaaacaacga ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga
      541 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
      601 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataacgg
      661 ctgaagcggc gcacgatcgt aatcgtgcgc ttccagcgtg gggcgggcga tttcaaaata
      721 cccctggctt gtttctattt gctcag
//
LOCUS       DBKEKI010000363          740 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.371, whole genome shotgun sequence.
ACCESSION   DBKEKI010000363 DBKEKI010000000
VERSION     DBKEKI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.371"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..502)
                     /locus_tag="ACUQPX_004510"
     CDS             complement(<1..502)
                     /locus_tag="ACUQPX_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HHV9058909.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLV"
BASE COUNT          208 a          167 c          181 g          184 t
ORIGIN      
        1 gaaccagcag ttgcagatgc tgcgcatcca gacctgccag tgcaaacagt tcagcgttga
       61 tgatttgatc aacttgttgc agttcagcga aatgattgcc accaccgata gagccaagcg
      121 agctgcgcca cggatgctgt gcaaatgctg acggcaggtt ttcctccagc cagctttctt
      181 cagcaacgtc atccaaagct gataatcgct tttcaaactt atcggcgttg tatttgcgag
      241 cgagaatatc tgtttgccat agcgccatgc cacagccgat atcattgccg accagtgctg
      301 ggtaaaaacg gcctgcggag aagaacgctg cgccaatcgg gtagccgcgt ccggggtgta
      361 aatctggcat ccccactacg cgctgcatgt tgggtaaatt tgccgtggtg tgtaattgtt
      421 ggatcgctaa actctcgatc cacaggtcat cagatgcgat ggtaaatacc gcatcggata
      481 agggacgaat ataattgccc atataccatt ccgtaaatag actcgtcata cttcaagttg
      541 catgtgctgc gacttgaatt attgagagta acaattaaaa aatcaggaaa tggcagacga
      601 atctagggca agaaaaaaag aattaatcct ggaaaggtct gcaaagagtc attgataaag
      661 gatttgtcat gatttacctc cttgcagttt gtggaataag aaaacgcgca catactaggc
      721 gggcgttatt ttcattgcaa
//
LOCUS       DBKEKI010000364          738 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.372, whole genome shotgun sequence.
ACCESSION   DBKEKI010000364 DBKEKI010000000
VERSION     DBKEKI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.372"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(236..>738)
                     /gene="aqpZ"
                     /locus_tag="ACUQPX_004511"
     CDS             complement(236..>738)
                     /gene="aqpZ"
                     /locus_tag="ACUQPX_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HHV9058910.1"
                     /translation="VTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGF
                     DAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIG
                     LALTLIHLINIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRT
                     LLEKRD"
BASE COUNT          199 a          222 c          180 g          137 t
ORIGIN      
        1 ctgcaccccc gggctctggc gaacaaaatc gccgctgcgc ggtaccttca gcttatcccc
       61 ttcggcaacc gggtcgggcg cgaggtaacg tcctgtaaga cgcgccctca gcccacatcc
      121 atgtgggctg ccccggcttt cggggaacgc ttcagcgatt ttgacgccac caatacccgt
      181 gcggtttgta atgtcgaaga taaaacaacc agggcagata aaatgccaat gctaattaat
      241 cacgcttttc cagcagagtc cggtaaatca gaccaccgat aatgccgccg acaattggca
      301 ccacccagaa gaaccacagt tgttctaatg cccagccgcc ctggaagata gcaaccgcgg
      361 tgctgcgcgc cgggttaaca gaagtgttag tcaccggaat attgattaag tgaatcaggg
      421 ttaaggccag accaatagcg atcggcgcaa aacctgccgg agcgaatttg tcggttgcac
      481 cgtggatcac taacaggaag cccgcactca ataccagttc aactaccagc gcggaaagca
      541 tggaataacc gcctggtgaa tgctcgccat aaccgttaga agcaaaaccg ctggctgccg
      601 cgtcaaaacc cgttttacca ctggcaatta aatacagcag cgccgctgca acaataccgc
      661 cgacaacctg ggcaattacg tagccaacga cttcttttgc cggaaaacgt ccgccagccc
      721 ataaaccaat agtgaccg
//
LOCUS       DBKEKI010000365          724 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.373, whole genome shotgun sequence.
ACCESSION   DBKEKI010000365 DBKEKI010000000
VERSION     DBKEKI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.373"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..270)
                     /locus_tag="ACUQPX_004512"
     CDS             complement(<1..270)
                     /locus_tag="ACUQPX_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HHV9058911.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAF"
     gene            complement(372..>724)
                     /gene="ycdY"
                     /locus_tag="ACUQPX_004513"
     CDS             complement(372..>724)
                     /gene="ycdY"
                     /locus_tag="ACUQPX_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HHV9058912.1"
                     /translation="IGDECAVPPYRSAWVEDATEAEVRAFLSERGMPLADTPADHIGT
                     LLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFWRTMAPLTRDA
                     ISAMWDELEEDSEE"
BASE COUNT          188 a          209 c          177 g          150 t
ORIGIN      
        1 aaacgcgaca ataccggtta tgacataacc gagaatttcc agatgtggtg ccagtgcact
       61 accgtaaata atgaccatcg cccacaccac gccactaagc agggttgcag cggagatcgc
      121 cagccctttc agcccacctt gcgggcaagc aaagtaggcc gtacagccca ggaagcccgc
      181 ccagcttagt aagcccaggg aaacagccac ccatccccag ataccggaga gaatgcctgt
      241 tgtgattgca atagagagaa gtatgttcat gccgcgcatg atagcagaaa acgcggccat
      301 gaatgagatc gagcacacat tttatgcaac aacaaattat ctgttgcagt tatgagtgac
      361 gatgatcaca tttactcttc agaatcttct tccagctcgt cccacattgc actaatggca
      421 tcacgggtta gcggtgccat ggtgcgccag aaaggcgtgg ttgcatgggc ctccactttg
      481 ccgaggaacg caccacacca gggtaacaga tactcactga acagtgtttc cagtgcttcg
      541 ctctcatctt ccgttgactg atcttccagc caggaagccg caagcagcaa tgtgccgatg
      601 tgatcggctg gcgtatccgc cagtggcatc cctcgttcgg aaagaaaagc gcgcacttcc
      661 gcttccgttg cgtcctcaac ccatgcgcta cgatatggcg gcacggcaca ttcatcgccg
      721 ataa
//
LOCUS       DBKEKI010000366          722 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.374, whole genome shotgun sequence.
ACCESSION   DBKEKI010000366 DBKEKI010000000
VERSION     DBKEKI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.374"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..380
                     /gene="dinD"
                     /locus_tag="ACUQPX_004514"
     CDS             <1..380
                     /gene="dinD"
                     /locus_tag="ACUQPX_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HHV9058913.1"
                     /translation="AAQQAGVATATDFAIFQNHGYQGLYGGLDQKAIHQLKELKKSQK
                     ILDHMGSTELAANLFRATQTEEKLKRDSVNSKQQANTTHFDVGSKVRQTIQELGGTMP
                     EELPTPQVSIKQLENSVKITEKK"
BASE COUNT          235 a          160 c          158 g          169 t
ORIGIN      
        1 aggcagcaca gcaagctggg gtagcgacag ctactgattt cgccatattt cagaatcatg
       61 gttaccaggg gctgtatggt ggattagatc agaaagccat ccaccaactg aaggagctga
      121 aaaagagtca gaagatcctg gatcatatgg gttcaacaga actggcggct aatctctttc
      181 gagctaccca aacagaagaa aaactcaagc gggatagcgt gaattcaaaa cagcaagcta
      241 ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac cattcaggaa cttggcggaa
      301 ccatgcctga ggagttgccg accccgcagg tcagcatcaa gcagttggaa aacagtgtaa
      361 aaattacaga gaagaaatag cagaaacgaa aaactctttt tgttgattga gacacccgat
      421 gcgtaagcca aggtccaggt gcagtgaaca caatggctaa acattgcacc tttcttttcc
      481 cccctcagtt ttaacctatt ttttcttatg cattttctca gacaagaagt cagaagaatg
      541 catctacgct acagaaaata gcgatttcac ataactaaaa gttatcaatt tcctctcacc
      601 ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac cctgccagac agatttttag
      661 ggagagaacc atgctgttac acattttgta tttggttggc attactgccg aagccatgac
      721 gg
//
LOCUS       DBKEKI010000367          715 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.375, whole genome shotgun sequence.
ACCESSION   DBKEKI010000367 DBKEKI010000000
VERSION     DBKEKI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.375"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..206)
                     /locus_tag="ACUQPX_004515"
     CDS             complement(<1..206)
                     /locus_tag="ACUQPX_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058914.1"
                     /translation="MSENKSFSTAVPAVQISDSGLNVPDEADILSGRLADFSAALGGA
                     MSTSLSSPQGQLASSESAIIADKN"
     gene            complement(203..559)
                     /locus_tag="ACUQPX_004516"
     CDS             complement(203..559)
                     /locus_tag="ACUQPX_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058915.1"
                     /translation="MQSRSLLLDTGTWDILLDDTGNLAITDNPHAVAQDVACACSTFL
                     GECWYDSTAGIPYWPRILGHWPGTQLVNATLQQEALKLPTVSAAVCQVTADQTRTVTG
                     VLRITDTNNDIFTVLL"
     gene            complement(572..>715)
                     /locus_tag="ACUQPX_004517"
     CDS             complement(572..>715)
                     /locus_tag="ACUQPX_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HHV9058916.1"
                     /translation="GDTAVSKGLDVTGQSRLSGGSNIGGIDFGNHVHGGVESGGSTTQ
                     GPR"
BASE COUNT          159 a          205 c          184 g          167 t
ORIGIN      
        1 tcgtttttat ccgcaataat ggcgctttcg ctggatgcaa gttgtccctg cggactgctc
       61 agactggtac tcatggcccc gcccagcgcc gccgaaaaat cagcaagcct gccgctcaga
      121 atatccgctt catccggcac attcagcccg ctatccgaaa tctgtacagc gggcactgcg
      181 gtagaaaaag atttattttc actcatagca gtaccgtaaa aatgtcatta ttggtatctg
      241 taatacgcag cacacctgtt actgtccggg tttgatcagc tgtgacctgg cagacagcgg
      301 cgctcacggt cggcagttta agtgcttcct gttgcagagt ggcattcacc agttgtgtgc
      361 cgggccagtg tccgaggatg cgtggccagt aaggtatgcc agccgttgag tcgtaccagc
      421 actcccccag aaaagtactg cacgcacacg ccacatcctg agctaccgca tggggattat
      481 cagtaatggc aagatttccg gtatcatcaa gcaggatatc ccatgtcccg gtgtcaagaa
      541 gaagtgatcg tgactgcatg ttttctcctg tttaccgggg tccctgcgtg gttgagccgc
      601 cggactcaac gccgccgtga acgtggtttc caaaatcaat acccccgatg ttcgaaccgc
      661 cggaaagcct ggactgcccg gtaacgtcaa gccccttact gacggcggta tcgcc
//
LOCUS       DBKEKI010000368          689 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.376, whole genome shotgun sequence.
ACCESSION   DBKEKI010000368 DBKEKI010000000
VERSION     DBKEKI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.376"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..298)
                     /locus_tag="ACUQPX_004518"
     CDS             complement(<1..298)
                     /locus_tag="ACUQPX_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHV9058917.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKVLVKAGDTLLIESLDRLSRQDIDIASE"
     gene            complement(334..519)
                     /locus_tag="ACUQPX_004519"
                     /pseudo
     CDS             complement(334..519)
                     /locus_tag="ACUQPX_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          200 a          161 c          163 g          165 t
ORIGIN      
        1 gctcagaagc aatatcaata tcctgacgtg acagacggtc taaactctcg attagtagcg
       61 tatcaccagc tttaacaagc actttctcga tagcggttaa aaagtcacct agaccacctt
      121 tcaggtgctt accactaaaa gcacttttcc ccaaatcttt aaagctcaag gatgaatcca
      181 agtaaaactc agggttctta tcaagccagt cagtaaccag cttagattgt cggcgtacag
      241 agtccccttt cagttgtcgt tcacttgaaa atctgatgta gctgattgcc cgtctcatat
      301 tcaataccta aaatgatgtg tttaggaacc cattatactg aagacaatac cggaccacat
      361 attcagggcg gagtggccgg taacaccaag ctgctcaacg tagaggggta agaagggcat
      421 taccagactg aaggcggccc cggtaagaaa acagcctagc caggcgacga tcaggtttcg
      481 tttccagttt ataggggtgt cattttcaca gggtgacata gcaatccgct gttggtgcgc
      541 caggcgcggt gaacataaga agaaaagata aacacactaa ttatgcgccc gacttccagg
      601 gggcgcaatc cagagagctt ttatcgctaa atcaggggga tttgctgtgg taatgccgga
      661 tgccattctg aagcatccgg catgggaga
//
LOCUS       DBKEKI010000369          683 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.377, whole genome shotgun sequence.
ACCESSION   DBKEKI010000369 DBKEKI010000000
VERSION     DBKEKI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.377"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..128
                     /locus_tag="ACUQPX_004520"
     CDS             <1..128
                     /locus_tag="ACUQPX_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HHV9058918.1"
                     /translation="GRESAPDASSVIHATGGDRVATSQTDRTAWHPDITRDKTRE"
     gene            125..>683
                     /locus_tag="ACUQPX_004521"
     CDS             125..>683
                     /locus_tag="ACUQPX_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HHV9058919.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQL
                     DIINTAFKVIETIRPKLACSRCDVIV"
BASE COUNT          200 a          176 c          176 g          131 t
ORIGIN      
        1 cgggaaggga aagtgcacct gacgccagct cagttatcca tgctactgga ggggatcgcg
       61 tggcaacatc ccaaacggac agaacggcct ggcatccgga tataacccgt gataaaacaa
      121 gggaatgaac aatgaactcc ccgatgatat tgagctgctt aaagccatgt tgcgtaagca
      181 acagagtcgg cttcgacagt atgcctgtca ggtcgcgggc tatgagcagg aaattgaacg
      241 gctgaaagcg caactcgaca ggttgcgtcg tatgttgttc ggccagagtt cagagaaaaa
      301 gcgtcataag cttgaaaatc agatccgaca ggcagaaaaa cgactgtcgg aactggaaaa
      361 ccggctgaac acagccagaa atcttctgga agatgcatcg tcagtcacag attcacctga
      421 caccagtccc ccgtcagaaa acccgatcgc cagtaagcct gaatccccgg gacgaaaatc
      481 ttcacgaaaa ccgctgccgg cagaacttcc ccgggagaca catcgccttc tgcctgctga
      541 aaccagttgc ccggcctgtg gaggtgttct gaaagaaatg ggggaaacaa tctcagagca
      601 actggatatc attaataccg cctttaaagt tatcgaaacc atacgtccca aactggcctg
      661 tagccggtgt gatgtcatcg ttc
//
LOCUS       DBKEKI010000370          680 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.378, whole genome shotgun sequence.
ACCESSION   DBKEKI010000370 DBKEKI010000000
VERSION     DBKEKI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.378"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..656
                     /locus_tag="ACUQPX_004522"
     CDS             <1..656
                     /locus_tag="ACUQPX_004522"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HHV9058920.1"
                     /translation="GGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIF
                     GPQKGASEAMIVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELK
                     SGIEIVTTALNLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAG
                     SLTDDVGVVHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
BASE COUNT          160 a          155 c          203 g          162 t
ORIGIN      
        1 gcggcggtag tcttaatact ctgaatgata ttgatatttc cggcctcgat ccgcgcttaa
       61 aagattgcgt cattcgcgtc gcttgtgatg tcaccaatcc gctggtgggc gataacggcg
      121 catcgcgcat ctttggccca caaaagggag ccagtgaagc gatgattgtt gagctggaca
      181 ataacctctc tcactatgcc gatgtcatta aaaaagcgct gcatgttgat gtaaaagatg
      241 tccccggcgc aggagctgcg ggcggtatgg gtgcggcgct gatggcgttt cttggtgcgg
      301 aactgaaaag tggtattgaa atcgtcacta cggcgctgaa tctggaggaa catattcacg
      361 attgtacgct ggtgatcacc ggtgaagggc gtattgacag ccagagtatt cacggcaagg
      421 taccgattgg cgtcgcaaac gtggcgaaga agtaccataa accggtgatt ggcattgcgg
      481 gtagcctgac cgatgatgtt ggcgttgtac atcagcatgg cattgatgcg gtcttcagcg
      541 tattgaccag cataggtacg ttggacgaag cattccgcgg ggcttatgac aatatctgcc
      601 gtgcttcacg taatattgcc gcgacactgg cgattggaat gcgcaacgcg gggtgacaag
      661 ggcgcgcaaa ccctctatac
//
LOCUS       DBKEKI010000371          668 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.379, whole genome shotgun sequence.
ACCESSION   DBKEKI010000371 DBKEKI010000000
VERSION     DBKEKI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.379"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            295..582
                     /locus_tag="ACUQPX_004523"
     CDS             295..582
                     /locus_tag="ACUQPX_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HHV9058921.1"
                     /translation="MAQVYFDTLKFVETLEAAGMPAAQARAISAAVKDSHEAAEVATK
                     RDLNDVRTELKNDIAALDAKIDKLSLQLTVRFGGMLVVAVGALTAIIKLPL"
BASE COUNT          179 a          186 c          162 g          141 t
ORIGIN      
        1 accgacgacg ccaatcgaac tggtggcgaa ctgtgcaggg ttggcgtaac cgggtacgga
       61 aaccggcgag cctttcggct cccccacaca gcccgccata aatcgcgaat gtgactatgc
      121 aaacgatatg aaaaaagacg cgggcgcgtc tcatatcgct ccgtaaacat ccggggtgct
      181 aatcccgacg ccagattttg ctggcgcgtg aggaatatag ccccggatta acgttgtcgt
      241 caacagccac tacaggctaa actaacaata ttccccaaca acgaaggatt taacatggct
      301 caggtctatt ttgatacact gaagttcgtc gaaaccctgg aagccgcagg aatgccggca
      361 gctcaggcgc gcgctatatc tgccgcagtg aaagattctc acgaagccgc agaagtcgcc
      421 acaaagcgcg acctgaatga tgtcagaact gaactaaaaa acgacattgc agcccttgat
      481 gcaaaaatag acaaactcag cctgcaactg accgtcagat ttggtggcat gctcgtcgtt
      541 gctgttggcg cactgacagc catcatcaaa ttacctcttt aaattaccag cccgttcggg
      601 gctggtttac cttaccccgt tactttattc gtacccctta taatggggtg ttagccagcc
      661 agacccgg
//
LOCUS       DBKEKI010000372          667 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.380, whole genome shotgun sequence.
ACCESSION   DBKEKI010000372 DBKEKI010000000
VERSION     DBKEKI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.380"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            39..203
                     /locus_tag="ACUQPX_004524"
     CDS             39..203
                     /locus_tag="ACUQPX_004524"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013964.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HHV9058922.1"
                     /translation="MCRQRITHETGWNVHHKVKKVMGGGEELSNLVLLHPNCHRQLHS
                     CEAGSLKKGL"
     gene            345..566
                     /locus_tag="ACUQPX_004525"
                     /pseudo
     CDS             345..566
                     /locus_tag="ACUQPX_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          193 a          169 c          167 g          138 t
ORIGIN      
        1 caggctgcct actcgagtca caaggctctc tgtgtccgat atgccggcag cgcattaccc
       61 atgaaactgg ctggaacgta caccataagg tgaagaaagt tatggggggc ggtgaagaat
      121 tatctaacct tgtcttgtta catccaaatt gccatcgaca gttacacagt tgtgaagccg
      181 gctctctcaa aaagggctta taaaggcttg agggtgtacc gggaaaccgg tatgcaccgt
      241 tcttagggga gggcagtcca gcaatgaact gtccttaccc gacaaacatc caaaacgaac
      301 ggaacgtgct gaaatccgca tataacccgt tgtaaagtga ggaaatggac acctcacttg
      361 ctcatgagaa cgcccgcctg cgggcactgt tgcagacgca acaggacact atccaccaga
      421 tggctgaata caaccgcctg ctctcacagc ggatggcggc ttatgcttcc gaaatcaacc
      481 gactgaaggc gctggttgcg aaactgcagc gtatgcagtt cgataaaagc tcagaaaaac
      541 ttcgcgcaaa aaccgaacgg cagattccat tttcacgggc gatctacgcc acacaggctc
      601 tgggttgcag tgactgctcc attaaggcga ttttgaactc ataagggaag taacaaaaaa
      661 actgcaa
//
LOCUS       DBKEKI010000373          667 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.381, whole genome shotgun sequence.
ACCESSION   DBKEKI010000373 DBKEKI010000000
VERSION     DBKEKI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.381"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..116)
                     /locus_tag="ACUQPX_004526"
     CDS             complement(<1..116)
                     /locus_tag="ACUQPX_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial assembly protein"
                     /protein_id="HHV9058923.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMV"
     gene            complement(197..>667)
                     /locus_tag="ACUQPX_004527"
     CDS             complement(197..>667)
                     /locus_tag="ACUQPX_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9058924.1"
                     /translation="VDTGTLTIKGNIAESPCKFEAGGDSVSINMPTVPTTVFEGKAKY
                     STYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIASSNDTVGYYLYLGDN
                     SDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVLSSLVMRVAQD"
BASE COUNT          193 a          143 c          123 g          208 t
ORIGIN      
        1 tgcaccataa cttctttatt ttctgccggg taaataatgc gcgtcccgta gataacaatt
       61 ccggcatgtg caggaaggac catagaaaaa atgagtaaag ataataatcc tttcatctta
      121 atgcctctta aattaaaaag ccgccatggg gatagcggcc atgtttatta cctggagaaa
      181 cttgcaattt tatttattaa tcctgcgcca cacgcataac cagagaagat aacacatcac
      241 ctgattgcac tgagttatct gtcagtttca ggtattttgc tttaaacgga atagcatatt
      301 gaccttccgc tgttttataa ctctcaatgt taaaaggtgc agaaacatcc aggacatcgc
      361 tgttatcacc taaatagaga tagtaaccca cggtatcgtt gctactggct atcgccttat
      421 cgttaccggt tattttgtcg tttgtgatca acgagagttt tacaccagca acttctttcg
      481 ggcagctaat ttttaacatg ctgctggtta caccgactgc atcatcatag gtagaatatt
      541 tagctttacc ttcaaagaca gtggttggta cagtcggcat attaatactt actgaatcac
      601 cacccgcttc gaatttacac ggagattctg caatatttcc tttaatggtt aatgttccgg
      661 tatcaac
//
LOCUS       DBKEKI010000374          666 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.382, whole genome shotgun sequence.
ACCESSION   DBKEKI010000374 DBKEKI010000000
VERSION     DBKEKI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.382"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            16..582
                     /locus_tag="ACUQPX_004528"
     CDS             16..582
                     /locus_tag="ACUQPX_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HHV9058925.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLLRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            586..>666
                     /locus_tag="ACUQPX_004529"
     CDS             586..>666
                     /locus_tag="ACUQPX_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HHV9058926.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKI"
BASE COUNT          139 a          168 c          165 g          194 t
ORIGIN      
        1 gtattggatg cacttatgtc attcacaaat acccctgaac gctatggtgt tatatctgcg
       61 gccttccatt ggctaagcgc aattattgtc tacggcatgt ttgctttagg tctgtggatg
      121 gtcacgctca gttattacga tggctggtat cacaaagcac ccgaactgca taaaagcatc
      181 ggtattttgt taatgatggg gctggttatt cgcgttctgt tgcgtgtcat atctccaccg
      241 ccgggtccgc tgccgagtta ttcgcccatg actcgtcttg ccgcaaaagc tggacatctc
      301 gcattgtacc tattgctgtt cgccatcggc atcagcggct atctgatctc aactgccgat
      361 ggtaaaccga tcagcgtttt tggctggttt gacgtccccg cgacccttgc cgacgctggc
      421 gcacaggcag actttgccgg tgccctgcat ttttggctcg cgtggagcgt cgtagtactg
      481 tctgttatgc acggatttat ggccctgaag catcatttca tcgataaaga cgacactctg
      541 aagcgcatgc tgggaaagtc gtcatctgac tatggagtat aaaaaatgaa aaaaagcctg
      601 cttggtttaa ccttcgcgtc cctgatgttc tctgccggtt cagcggttgc cgccgattac
      661 aaaatt
//
LOCUS       DBKEKI010000375          659 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.383, whole genome shotgun sequence.
ACCESSION   DBKEKI010000375 DBKEKI010000000
VERSION     DBKEKI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.383"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>659
                     /locus_tag="ACUQPX_004530"
     CDS             <1..>659
                     /locus_tag="ACUQPX_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HHV9058927.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNELPASPVEWLTDN
                     GSCYRANETRQFARMLGLEPKSTAVRSPESNGIAESFVKTIKRDYISVMPKPDGLTAA
                     K"
BASE COUNT          161 a          162 c          205 g          131 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggatca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aacccgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgtg aggcactgca
      361 ctgggcggtc actacgggcg gcttcaacag tgaaacagta caggacgtca tgctgggagc
      421 ggtggaacgc cgcttcggca acgagcttcc ggcgtctcca gtagagtggc tgacggataa
      481 tggttcatgc taccgggcta atgaaacacg gcagtttgcc cggatgttgg ggcttgaacc
      541 gaagagcacg gcggtgcgga gtccggagag taacggcata gcagagagct tcgtgaaaac
      601 gataaagcgt gactacatca gtgtcatgcc caaaccagac gggttaacgg cagcaaaga
//
LOCUS       DBKEKI010000376          658 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.384, whole genome shotgun sequence.
ACCESSION   DBKEKI010000376 DBKEKI010000000
VERSION     DBKEKI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.384"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            92..274
                     /locus_tag="ACUQPX_004531"
     CDS             92..274
                     /locus_tag="ACUQPX_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HHV9058928.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     RVTELFALLHTEEGCC"
     gene            complement(375..656)
                     /locus_tag="ACUQPX_004532"
     CDS             complement(375..656)
                     /locus_tag="ACUQPX_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HHV9058929.1"
                     /translation="MFRRKNKLQREIQDVEKKIRDNQKRVLLLDNLSDYIKPGMSVEA
                     IQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGEMKNGEAK"
BASE COUNT          169 a          145 c          162 g          182 t
ORIGIN      
        1 ccgttcagac ggcaggtgcc tgcttttccg gcaacaagca ggaatctcag cgctgaagca
       61 ggaattagag gtaaaaacgc cctaccgggc catgaatcac ccggtcattg gagtagtaac
      121 caaagcagat ttagccagca tggaacaaat atctttggtg aaaagttggt tacgggaagc
      181 tggagcgcac aacgtgttag taaccagtgc agttaataac aatagagtta cagaactttt
      241 tgccttgctg catacagaag aaggctgttg ttaatttcga ttggttctca gatgagaatg
      301 atgacggtga tacacagata agaatcaatg agcaatgagg gttgccgggc aaccctcatt
      361 gaataaaacg ggaattactt cgcttcgccg tttttcattt cgcccatagc tttcagcttt
      421 ttggagatat cgcggcgttc tttggagagc tcggcatttt tgatgatgta atcgtcaacg
      481 cgatcttcat agtcaccttt catgctggcg atgatgccct ggattgcttc aacgctcatc
      541 cctggcttga tgtaatcgct caggttgtcc agcaacagga cgcgcttctg gttgtcacgg
      601 atctttttct caacgtcctg aatttcacgt tgcagtttgt tcttacgacg gaacagac
//
LOCUS       DBKEKI010000377          656 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.385, whole genome shotgun sequence.
ACCESSION   DBKEKI010000377 DBKEKI010000000
VERSION     DBKEKI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.385"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(27..614)
                     /gene="cbrC"
                     /locus_tag="ACUQPX_004533"
     CDS             complement(27..614)
                     /gene="cbrC"
                     /locus_tag="ACUQPX_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HHV9058930.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
BASE COUNT          193 a          168 c          117 g          178 t
ORIGIN      
        1 ccggcctgtt ctcattattt aaataactac gaaaaatcac cccacagtct cagcttgccg
       61 cagtggagac agcggaagag ataaccctga caatcaccac ccttttgcag gcatttagct
      121 agatcagaat ttctaatacc gaaattctca caatcttctt caaggttggc aaattcatcg
      181 aggcgatctt ttatatcatt ccagcccaca tagccgataa aagcacagaa atcgccacaa
      241 tgcgcaagcc agaattcctg ttgccatcca tgataacctg gcgtgcgttc aaccaactct
      301 ttcagcattt catcaggata tgtgttctta ataccggcaa attcgtcctc ttcatcatac
      361 tcaaattcaa caccttctat gctggcatca tcctgaaaac tacctgcaaa cttttcagca
      421 gcagaaccgt ccgcaataca caacggacag agatgttcaa cttcatcaac gcaataaaag
      481 ggacccgagt aataaactga cgtctgttgt tcacagcaat cgcactctac ggttttatcc
      541 tgttcaaatg cgcctgtttc cagtggcttg ggatgatatt tgaattgggg taacggcctg
      601 atattttgag tcatagtaac atcctgtctg cttttgagta gcccaaagat aaagta
//
LOCUS       DBKEKI010000378          655 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.386, whole genome shotgun sequence.
ACCESSION   DBKEKI010000378 DBKEKI010000000
VERSION     DBKEKI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.386"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            68..>655
                     /gene="fimH"
                     /locus_tag="ACUQPX_004534"
     CDS             68..>655
                     /gene="fimH"
                     /locus_tag="ACUQPX_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="involved in the regulation of fimbriae length and
                     mediates adhesion of type 1 fimbriae; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HHV9058931.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPRQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGEMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGLMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVP"
BASE COUNT          179 a          127 c          141 g          208 t
ORIGIN      
        1 cttacagcaa gccgtcactg ttatttcggc agtttgcaca catcctggct cataaaggaa
       61 attatcaatg aagataatct gcagattatt attggcgatg gcatgtttgt gtctggcaaa
      121 catatcctgg gctactgttt gtgcaaatag tactggcgta gcagaagatg aacactatga
      181 tctctcaaat atctttaata gcaccaataa ccagccaagg cagattgttg ttttaccgga
      241 aaaatccggc tgggtaggtg tctcagcaat ttgtccaccc ggtacgctgg tgaattatac
      301 ataccgtagt tatgtcacca actttattgt tcaggaaact atcgataatt ataaatatat
      361 gcaattaaat gattatctat taggtgagat gagtctggtt gatagtgtga tggatattca
      421 gttccccccg caaaattata ttcggatggg aacagatcct aacgtttcgc aaaaccttcc
      481 attcgggctg atggattctc gtttaatatt tcgtttaaag gttattcgtc cctttattaa
      541 catggtggag atccccagac aggtgatgtt taccgtgtat gtgacatcaa cgccttacga
      601 tccgttggtt acacctgttt ataccattag ttttggtggc cgggttgaag taccg
//
LOCUS       DBKEKI010000379          652 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.387, whole genome shotgun sequence.
ACCESSION   DBKEKI010000379 DBKEKI010000000
VERSION     DBKEKI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.387"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..471
                     /gene="ligB"
                     /locus_tag="ACUQPX_004535"
                     /gene_synonym="yicF"
     CDS             <1..471
                     /gene="ligB"
                     /locus_tag="ACUQPX_004535"
                     /gene_synonym="yicF"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="this ligase is similar to LigA but it lacks the
                     C-terminal BRCT domain; catalyzes strand joining of nicked
                     DNA in the presence of a divalent cation and NAD+; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HHV9058932.1"
                     /translation="ASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHI
                     FSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDE
                     RSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(468..>652)
                     /locus_tag="ACUQPX_004536"
     CDS             complement(468..>652)
                     /locus_tag="ACUQPX_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HHV9058933.1"
                     /translation="KELYAGVSFASAVLYIALQHYVSNHDVVIISTLVFGFFARLLAL
                     RLKLGLPVFYYSHEGH"
BASE COUNT          166 a          146 c          187 g          153 t
ORIGIN      
        1 gcttctgatg tttgtcagga acagttcatt tcacgcttag tctggctggg gtcaaaacag
       61 gttctggggc tggatggcat tggtgaggcc ggttggcgcg cgctgcatca gactcatcgc
      121 tttgagcata tcttttcctg gcttttatta acgccagagc aattacagaa cacgccgggg
      181 atcgcgaaaa gtaaaagtgc gcagttatgg catcagttta atctggctcg taaccagcct
      241 tttactcgct gggtgatggc aatgggaata ccgctaaccc gggcggcgct taatgccagt
      301 gatgaacggt cctggtcgca acttttattt agcacggagc agttctggca gcagctgccg
      361 gggactggat ctgggcgcgc cagacaggtt attgaatgga aggaaaatgc gcaaatcaag
      421 aagctgggca gttggctggc tgcccagcag atcacaggtt ttgaacctta gtgaccttcg
      481 tggctgtagt aaaagaccgg taatcccagc ttaagacgta gcgccagtaa acgagcaaaa
      541 aagccgaata ctagggtgga gataatcacc acatcatggt tagaaacata gtgttgcagc
      601 gcgatgtaca gcacggcgga ggcaaatgag acaccggcat acaactcttt tt
//
LOCUS       DBKEKI010000380          649 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.388, whole genome shotgun sequence.
ACCESSION   DBKEKI010000380 DBKEKI010000000
VERSION     DBKEKI010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.388"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(54..242)
                     /locus_tag="ACUQPX_004537"
     CDS             complement(54..242)
                     /locus_tag="ACUQPX_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058934.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            358..649
                     /locus_tag="ACUQPX_004538"
                     /pseudo
     CDS             358..649
                     /locus_tag="ACUQPX_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          172 a          154 c          181 g          142 t
ORIGIN      
        1 gtgaacagac gtttcagcgg gcggggagta aaagcggaca acatgaggac ggattaaccc
       61 ccggtatcct gtagccgggt aagggctgct gtgtcgccac ggcgctccag gatggaggcc
      121 gcgaccatcg caggtggtgg gtccatgggt ttgagcatct tcatccagtc ccattcacgt
      181 tgtgagagct tctcagcacc aacagagagc agaacggtat ctatactttt gcgggtcaac
      241 ataagaggat gagtccttaa taaaacaggt cctcagagca tacatattct gcggagcgtg
      301 cggcaacaac aggacgaaca ggggcggttt tcaatttgca gccgtcaggc tgccgtggtt
      361 cagcgcctga tgggaatcct ctcacttaag gcagcgatta aggtcaagcg ataccgctct
      421 tacagaggag aggtagggca aaccgccccc aatgttctcc aaagagattt caaggctacg
      481 cggccaaacg agaagtgggt taccgatgtt actgaatttg cagtcaatgg gcgcaagctg
      541 tatttgcctc cagtaataga tctcttcaac aacgaagtta tttcttacag cctttcggaa
      601 agaccagtga tgaacatggt cgagaatatg ctcgatcagg cattcaaaa
//
LOCUS       DBKEKI010000381          642 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.389, whole genome shotgun sequence.
ACCESSION   DBKEKI010000381 DBKEKI010000000
VERSION     DBKEKI010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.389"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            24..>642
                     /gene="tetR(B)"
                     /locus_tag="ACUQPX_004539"
     CDS             24..>642
                     /gene="tetR(B)"
                     /locus_tag="ACUQPX_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HHV9058935.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
BASE COUNT          213 a          115 c          140 g          174 t
ORIGIN      
        1 tcaacaaaaa ttaggaatta atgatgtcta gattagataa aagtaaagtg attaacagcg
       61 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
      121 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg
      181 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
      241 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
      301 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
      361 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg
      421 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
      481 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
      541 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
      601 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gt
//
LOCUS       DBKEKI010000382          616 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.390, whole genome shotgun sequence.
ACCESSION   DBKEKI010000382 DBKEKI010000000
VERSION     DBKEKI010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.390"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>616
                     /locus_tag="ACUQPX_004540"
     CDS             <1..>616
                     /locus_tag="ACUQPX_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HHV9058936.1"
                     /translation="QINPDFSSGRFQDAIGRIYFLERRGATGTTVTATCTGLVGTLIP
                     AGSMAQDEAGYKYVSQSDVTIGASGQVDAVFLNLSTGPVGCPVGTLNKIYKAIPGWSG
                     VTNASAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVENVVDVYVTHNP
                     KKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDM"
BASE COUNT          142 a          155 c          184 g          135 t
ORIGIN      
        1 cagatcaacc ctgacttttc cagtggtcgc tttcaggatg caataggcag gatttatttc
       61 ctggagcgcc gcggggccac aggtacgaca gtaacagcga cctgtaccgg gctggttggc
      121 acgctgatcc cggcgggcag tatggcgcag gatgaggccg gttataagta tgtcagtcag
      181 tcagacgtca ctatcggcgc atcagggcag gttgatgcgg tatttctgaa tttgtccacc
      241 ggccccgtcg gctgtcctgt ggggacgctg aataaaattt ataaggcaat acctggctgg
      301 tcaggtgtca ctaacgccag tgccggtgtg ccgggcagcg atgaggaaac ccgtgcggac
      361 tttgaaaacc gccgacgtaa ttcagttgcc cgtaatgccc ggaatattct ggaagccatc
      421 cggggtgaaa tactctccac ggtggaaaac gtggtggatg tttacgtcac ccataatccg
      481 aaaaaaacag aacaaaaagc cggagtcagt cagtatccat taacacccgg ttcgctttat
      541 gttggcgtgt acggcggcag cccggcagat atcgcggcgg ccatctggcg taaggctccg
      601 ccgggtattg atatga
//
LOCUS       DBKEKI010000383          609 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.391, whole genome shotgun sequence.
ACCESSION   DBKEKI010000383 DBKEKI010000000
VERSION     DBKEKI010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.391"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..>609)
                     /gene="ipaH3"
                     /locus_tag="ACUQPX_004541"
     CDS             complement(<1..>609)
                     /gene="ipaH3"
                     /locus_tag="ACUQPX_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HHV9058937.1"
                     /translation="AVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNAL
                     ISLPELPASLEYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNN
                     QLTMLPELPTSLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEET
                     EIFFRCRENRITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          162 a          102 c          167 g          178 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgct
//
LOCUS       DBKEKI010000384          608 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.392, whole genome shotgun sequence.
ACCESSION   DBKEKI010000384 DBKEKI010000000
VERSION     DBKEKI010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.392"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..608
                     /locus_tag="ACUQPX_004542"
                     /pseudo
     CDS             1..608
                     /locus_tag="ACUQPX_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          160 a          148 c          170 g          130 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccggtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccggggcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt attcagggcg cgcattgcgc aacgagctga
      541 aaaataacgg tataaaggca gtaatccccc gaaagtcaaa tgagaaaatg gcatcggatg
      601 gacgtgca
//
LOCUS       DBKEKI010000385          604 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.393, whole genome shotgun sequence.
ACCESSION   DBKEKI010000385 DBKEKI010000000
VERSION     DBKEKI010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.393"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..565
                     /gene="kduD"
                     /locus_tag="ACUQPX_004543"
     CDS             <1..565
                     /gene="kduD"
                     /locus_tag="ACUQPX_004543"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HHV9058938.1"
                     /translation="RKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDALEFSEKDWD
                     DVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTASKSGVMGV
                     TRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRWGLPSDLMG
                     PVVFLASSASDYVNGYTIAVDGGWLAR"
BASE COUNT          146 a          141 c          167 g          150 t
ORIGIN      
        1 gcgaaagatt gatggcattc ctggactgct ggatcgcgcg gtagctgaat ttggtcatat
       61 tgatatcctg gtgaataacg ccggattgat tcgccgcgaa gacgctctcg agttcagcga
      121 aaaagactgg gacgatgtca tgaacctgaa tatcaagagc gtattcttca tgtctcaggc
      181 agcggcgaaa cactttatcg cgcaaggcaa tggcggcaag attatcaata tcgcgtcaat
      241 gctctccttc cagggcggga tccgtgtgcc ttcttatacc gcatcaaaaa gcggcgtgat
      301 gggtgtgacg cgattgatgg caaacgaatg ggctaaacac aacattaatg ttaatgcgat
      361 agctccgggt tacatggcga ccaacaatac ccaacaactg cgggcagatg aacaacgtag
      421 cgcggaaatt ctcgatcgca ttccagctgg ccgttgggga ctgccgagtg acctgatggg
      481 gccggtagtg tttcttgcct ccagcgcttc agattatgta aatggttata ccattgctgt
      541 ggatggcggt tggctggcgc gttaattcat tcctcttact tttatgaccc tgccgcatgg
      601 cagg
//
LOCUS       DBKEKI010000386          598 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.394, whole genome shotgun sequence.
ACCESSION   DBKEKI010000386 DBKEKI010000000
VERSION     DBKEKI010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.394"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            255..>598
                     /locus_tag="ACUQPX_004544"
     CDS             255..>598
                     /locus_tag="ACUQPX_004544"
                     /inference="COORDINATES: protein motif:HMM:NF015371.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHV9058939.1"
                     /translation="MRAETAAKRAEDIADVVSLEDASTTKKGIVQLSSATNSASESLA
                     ATAKAVKVVMDETNKKAPLNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAAIVDS
                     SPDALNTLNELAA"
BASE COUNT          158 a          167 c          184 g           89 t
ORIGIN      
        1 ccagggatgc cggcacatct gccagtgagg cggcaaccaa tgcgactgat gctgcaggct
       61 cagcacgcgc agccagcacg tcagccggtt cggcagcgtc atcggcatca tctgcgtctg
      121 cttcaaaaga tgaggcgact aaacaggcgt cagcagcgaa gggcagtgcc acgacggcat
      181 cctctaaggc gacagagtct gcaggcagtg cgacggcggc agcgcagagc aaaagcgcgg
      241 cagaatccgc ggcaatgcgc gctgagacag cagcaaaacg ggcagaggat attgctgatg
      301 tcgtatctct ggaagatgcg agcacgacga aaaaggggat agtgcagctc agcagtgcga
      361 ccaacagtgc atccgagtca ctggccgcga cagcgaaagc ggttaaggtg gtaatggatg
      421 aaacaaacaa gaaagcgccc ctgaacagtc cggctctgac cggaacgcca accacaccca
      481 ctgcgccaaa ggggactaat aatactcaga tcgccagtac ggcttatgtt atggccgcga
      541 ttgctgccat cgtggactcg tcgcctgatg cactgaacac gctgaatgaa ctggcggc
//
LOCUS       DBKEKI010000387          596 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.395, whole genome shotgun sequence.
ACCESSION   DBKEKI010000387 DBKEKI010000000
VERSION     DBKEKI010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.395"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..283
                     /locus_tag="ACUQPX_004545"
     CDS             <1..283
                     /locus_tag="ACUQPX_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HHV9058940.1"
                     /translation="IKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDS
                     AMQVLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            412..>596
                     /locus_tag="ACUQPX_004546"
     CDS             412..>596
                     /locus_tag="ACUQPX_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HHV9058941.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLS"
BASE COUNT          136 a          159 c          174 g          127 t
ORIGIN      
        1 gatcaaatta aactccatga aaaacatgct gtatgaagca gcgtggaaag cagacaacgg
       61 caccatcacc tctggcgatg cagcgatgtg caaatacttc tgcgccaatg cggcatttga
      121 agtggtggat agcgcaatgc aggtgctggg cggtgtcggg attgcgggca accaccgcat
      181 cagccgcttc tggcgtgacc tgcgtgtaga ccgcgtttcc ggaggatctg acgaaatgca
      241 gatcctgacg ctgggtcgtg cggtgctgaa gcaataccgc taagtccgtg cgctgcctga
      301 tgcgacgctg acgcgtctta tcaggcctac gggagcacga atgtaggccg gataaggcgt
      361 ttacgccgca tccggcagtc atgcgctaca taaatttttc aggagctaat aatggatcat
      421 ctacccatgc cgaaattcgg accgctggcc ggattgcgcg ttgtcttctc cggtatcgaa
      481 atcgccggac cgtttgccgg acaaatgttc gcagaatggg gcgcggaagt tatttggatc
      541 gagaacgtcg cctgggccga caccattcgc gttcaaccga actacccgca gctctc
//
LOCUS       DBKEKI010000388          595 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.396, whole genome shotgun sequence.
ACCESSION   DBKEKI010000388 DBKEKI010000000
VERSION     DBKEKI010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.396"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(4..595)
                     /locus_tag="ACUQPX_004547"
                     /pseudo
     CDS             complement(4..595)
                     /locus_tag="ACUQPX_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
BASE COUNT          151 a          165 c          127 g          152 t
ORIGIN      
        1 agcttaaccg aacgctaatt ctgcggcagc ggctttatca gcgattacca tcagcgaagg
       61 atgcagctgc aacacggaag ctggaacatc ttcggtaacc ggaccttgca gcacattttt
      121 cagtgcctgc gctttacctg cgccactgac aattatcagt aagtttttcg ccgccatgat
      181 gcttttcggc cccatcgtca cgtagctgtc aggcactaac gaaaagtcgc cgcccaactc
      241 accatgcgcc acaatatcaa ccatctctcc ctggatcggg aactccacgg tttgctcatg
      301 gaaatgggtc gtattcggca aattgccaca aaaatgaatc tgcacctaat cccagcacca
      361 ccaaatccag gccgccttca cgcgccagtt tctgatcatg ctcgcggtag ttatcaatag
      421 tgagcttctg gatattctct tctttgatcc ccgcaggggt gaaaaacaga ttacgcagat
      481 tggtaatcgt tacgccttct ccctcttttc cgcgaaaggg aatttcatcg aaattataga
      541 aatagcagtt atcgtaccaa ggcttacctt taaccagggt ggtgaggtat tcata
//
LOCUS       DBKEKI010000389          591 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.397, whole genome shotgun sequence.
ACCESSION   DBKEKI010000389 DBKEKI010000000
VERSION     DBKEKI010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.397"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            432..>591
                     /locus_tag="ACUQPX_004548"
     CDS             432..>591
                     /locus_tag="ACUQPX_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HHV9058942.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKE"
BASE COUNT          205 a           87 c          117 g          182 t
ORIGIN      
        1 atgattggat gcttttgtgg tgaaaaatgt tttggtgttt tagcataacc ctgaagaaag
       61 aataaaaata aaaaggaaaa aaccaaaacc agaaaagaag agaaccggaa agagaagaaa
      121 agagaaaatg aaaaataaaa accaaaaaag aaagaatact taaatcttcg catgttcttc
      181 gggtggtacg caagtttgtt agtctgaccg cacaataaat tttcgcttag aaaataataa
      241 gttacataat attctgcttt tttagataaa tataaccaga aaaaatcatt gttaatctta
      301 tgcatttatt atttgattgt aagttgtgat tattataatt tagttattgt tagtatgttt
      361 tataatgctg gatgggtgat gcatctaatt agttgaatct gatgtataat acgggctttt
      421 gaggttatct catgaccagc gttaatattc attgtccccg ttgtcagtca gctcaggttt
      481 accgccatgg tcagaaccct aaaggccgtg acagacttcg ctgccgtgac tgccaccgtg
      541 tgtttcagtt cacttatact tatcaagcac gtaagccggg tatgaaagag c
//
LOCUS       DBKEKI010000390          591 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.398, whole genome shotgun sequence.
ACCESSION   DBKEKI010000390 DBKEKI010000000
VERSION     DBKEKI010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.398"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>591
                     /locus_tag="ACUQPX_004549"
     CDS             <1..>591
                     /locus_tag="ACUQPX_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HHV9058943.1"
                     /translation="TPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTM
                     KNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQYEEL
                     RKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG
                     EMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPEL"
BASE COUNT          177 a          149 c          170 g           95 t
ORIGIN      
        1 tacaccagag aacgatgcag ccttcccccg ccagacacat gccgggaacc cggcgctcta
       61 cccgcaggtc aaaatggtct gccagatgga actgaccagc catctgctga cggctgcagc
      121 cttcggcacg atgaagaaca gcgaaaatga gcttgctgag caacttatag aacaaaccgg
      181 cgataacact ctgacgttaa tggataaagg ttattactca ctgggactgt taaatgcctg
      241 gagcctggcg ggagaacacc gccactggat gatacctctc agaaagggag cgcaatatga
      301 agagctcaga aaactgggta aaggcgatca tctggtgaag ctgaaaacca gcccgcaggc
      361 acgaaaaaag tggccgggac tgggaaatga agtgacagcc cgcctgctga ccgtgacgcg
      421 caaaggaaaa gtctgccatc tgctgacgtc gatgacggac gccatgcgct tccccggagg
      481 agaaatggcg gatctgtaca gtcatcgctg ggaaatcgaa ctgggataca gggagataaa
      541 acagacgatg caactgagca ggctgacgct gagaagtaaa aagccggagc t
//
LOCUS       DBKEKI010000391          578 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.399, whole genome shotgun sequence.
ACCESSION   DBKEKI010000391 DBKEKI010000000
VERSION     DBKEKI010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.399"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..>578)
                     /locus_tag="ACUQPX_004550"
     CDS             complement(<1..>578)
                     /locus_tag="ACUQPX_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HHV9058944.1"
                     /translation="DTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFS
                     HKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTL
                     AVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSV
                     LFPYPGFQSGFVEVNDSSQGNQQGTTNIINGA"
BASE COUNT          143 a          157 c          124 g          154 t
ORIGIN      
        1 ctgcaccgtt aatgatgttt gttgtcccct gctgattgcc ctggctactg tcgttaacct
       61 caacaaaacc agactgaaat ccaggataag ggaacaacac actgtcactt tgaccaagct
      121 gcaaagttcg gctgcctgcc acccgcgctt cgagttgcag atcctgactc tcttcatcct
      181 gttcagagaa gcctttcaca tccaccagta ccgcacccag caaatcgtct gtaccactgg
      241 ctcccaaatt aacgccatag cgactgacag ccagcgtaga gctataagtg cccgtaaagg
      301 ccgaaagatg atcatgattc ttacggtcat aactatcaga tacggtagcg tagacatttc
      361 cgtattgccc atcccaacgc ccgttaaccg cgctgtttat ggtatcggtg ttcaggcctc
      421 cgacggttac gccaagttct ttatggctga atgaatcgtt ataaaaggta tgcgataact
      481 gccatgacgt ttgatcgcca tcctgttcgc tataacgata atccgtagac acattggttg
      541 aatggctgtt gttattgcta tccatcgttg gcgtgtca
//
LOCUS       DBKEKI010000392          576 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.400, whole genome shotgun sequence.
ACCESSION   DBKEKI010000392 DBKEKI010000000
VERSION     DBKEKI010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.400"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(86..576)
                     /gene="tqsA"
                     /locus_tag="ACUQPX_004551"
                     /pseudo
     CDS             complement(86..576)
                     /gene="tqsA"
                     /locus_tag="ACUQPX_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
BASE COUNT          162 a          135 c          132 g          147 t
ORIGIN      
        1 tatggttttt catattcata catttttatt agggatttat agctgtttaa ctaagtgtgg
       61 ttaattgcgt ggttaatttg acttatgtaa gcatgctgat ggctgttttc agcaccagat
      121 aatgagaaac actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca
      181 tcatttgctg aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca
      241 gcagcaataa aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca
      301 acgtcatcgc cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc
      361 ctacgcgttg caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt
      421 gcggtaacgt ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca
      481 tcgccatcac gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc
      541 ggaccatatg ttgcaccagc gggttaagaa taacag
//
LOCUS       DBKEKI010000393          559 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.401, whole genome shotgun sequence.
ACCESSION   DBKEKI010000393 DBKEKI010000000
VERSION     DBKEKI010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.401"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..322
                     /locus_tag="ACUQPX_004552"
     CDS             <1..322
                     /locus_tag="ACUQPX_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HHV9058945.1"
                     /translation="TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEI
                     TRHSAWDRMSQAGAQLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTL
                     TKQK"
     gene            complement(358..>559)
                     /locus_tag="ACUQPX_004553"
     CDS             complement(358..>559)
                     /locus_tag="ACUQPX_004553"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HHV9058946.1"
                     /translation="DYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLA
                     GELIGRGVFYGLHMTVGMAVAS"
BASE COUNT          150 a          132 c          149 g          128 t
ORIGIN      
        1 gacaggtaaa aaacagttaa ttattgccgg tgtggtaacc gaagtttgcg tggcattccc
       61 ggcgctttca gccattgaag aagggtttga tgtctttgtc gtgactgatg catcagggac
      121 ctttaatgaa attacccggc attcggcgtg ggatcgcatg tcgcaagctg gtgcgcaatt
      181 gatgacatgg ttcggcgtgg cttgcgagct gcaccgtgac tggcgtaatg atattgaagg
      241 actggcgacg ttgttctcta accatattcc tgattatcgt aatttgatga ccagttatga
      301 cacgttaacg aagcagaaat aaaagagaac cggggccgaa accccggttg ttactgatta
      361 gcttgcgacg gccatcccca cggtcatatg caaaccatag aatacgccgc gaccgattaa
      421 ttcccctgcc agaagcagaa tgaatgagac ggatagtagc ggaaccgcag gctgataacc
      481 ttttagctgt ggtgcaatcc acaagcacag ggcaacggcc aaaagcacga tccgccagga
      541 catcagcgca ccatagtcc
//
LOCUS       DBKEKI010000394          551 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.402, whole genome shotgun sequence.
ACCESSION   DBKEKI010000394 DBKEKI010000000
VERSION     DBKEKI010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.402"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>551
                     /locus_tag="ACUQPX_004554"
     CDS             <1..>551
                     /locus_tag="ACUQPX_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HHV9058947.1"
                     /translation="SYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMAT
                     RRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWC
                     GDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVM
                     FHSDQGSHYTSRQFRQLLWRYQI"
BASE COUNT          133 a          140 c          171 g          107 t
ORIGIN      
        1 agctacagat actggaaaaa ccgtcctgaa aaaccagacg gcagacgggc tgtattacgc
       61 agtcaggtac ttgagctaca tggcatcagc cacggttcgg ccggagcaag aagcatcgcc
      121 acaatggcaa cccggagagg ctaccagatg ggacgctggc ttgctggcag gctcatgaaa
      181 gagctggggc tggtcagctg tcagcagccg actcaccggt ataaacgtgg tggtcatgaa
      241 catgttgcta tccctaacta ccttgaaagg cagttcgccg tgaccgagcc aaatcaggtg
      301 tggtgcggtg atgtgaccta tatctggacg ggtaagcgct gggcgtacct cgccgttgtt
      361 ctcgacctgt tcgcaagaaa accagtgggc tgggccatgt cgttctcgcc ggacagcagg
      421 ctcaccatga aagcgctgga aatggcatgg gaaacccgtg gtaagcccgg cggggtgatg
      481 ttccacagcg atcagggcag tcattatacg agcaggcagt tccggcagtt attgtggcga
      541 taccagatca g
//
LOCUS       DBKEKI010000395          546 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.403, whole genome shotgun sequence.
ACCESSION   DBKEKI010000395 DBKEKI010000000
VERSION     DBKEKI010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.403"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..66
                     /locus_tag="ACUQPX_004555"
     CDS             <1..66
                     /locus_tag="ACUQPX_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HHV9058948.1"
                     /translation="RLMHAEGVFRTQEIWRKEGYL"
     gene            63..503
                     /locus_tag="ACUQPX_004556"
                     /pseudo
     CDS             63..503
                     /locus_tag="ACUQPX_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
BASE COUNT          163 a          103 c          163 g          117 t
ORIGIN      
        1 cgcctgatgc atgcagaagg tgtttttcgc acacaagaaa tctggagaaa ggaaggttat
       61 ttatgattta cccaacaaat acaggcaaaa gcggggaaca ccttcgtctc accacgctgg
      121 aaagtgtctg gattcaggga aaactgcgca tgtgggggcg ctggtcgtat attggcggcg
      181 gtaagacggg aaatatgttt aaccagttgc tggcatccaa aaaattgacg aaaacagccg
      241 tcaatgaagc cctgcgcagg atgaaaaaag cgggaataga gaaacctgag ctggaagcgt
      301 ttttgcgaga gatgatcaat ggcaagcaaa agacctggct ggcgcattgt actgatgcag
      361 aggcgttatg tattgataga gtcataagtg aggtgctggc agagcatcct ggattgatta
      421 gtgtcctccg gcaacggtat gaagggcggg ggatgactaa gcgtaaaatg gctgaattgc
      481 tgaatgacgc acaccctgag tggtgaatag cagacatgga cacttgaaag aacccgcttt
      541 atgcgg
//
LOCUS       DBKEKI010000396          546 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.404, whole genome shotgun sequence.
ACCESSION   DBKEKI010000396 DBKEKI010000000
VERSION     DBKEKI010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.404"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..115
                     /locus_tag="ACUQPX_004557"
     CDS             <1..115
                     /locus_tag="ACUQPX_004557"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="RNase BN; member of metallo-beta-lactamase family;
                     the purified enzyme from Escherichia coli forms dimeric
                     zinc phosphodiesterase; in Bacillus subtilis this protein
                     is a 3'-tRNA processing endoribonuclease and is essential
                     while in Escherichia coli it is not; associates with two
                     zinc ions; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HHV9058949.1"
                     /translation="THVSSRYDDKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            306..>546
                     /locus_tag="ACUQPX_004558"
     CDS             306..>546
                     /locus_tag="ACUQPX_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HHV9058950.1"
                     /translation="MVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDEE
                     SIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLS"
BASE COUNT          172 a          115 c           98 g          161 t
ORIGIN      
        1 tacccacgtc agctcgcgct atgatgacaa aggttgtcaa cacctgttac gtgaatgcag
       61 gtcaattttc ccggcgactg aactggcgaa tgatttcacc gtgtttaacg tttaactctg
      121 cttattatta aaaacagggt gaaacttgcc ctgttatagc aacccgcgct acatatatcc
      181 acccacacag gcatattgag cgatttttga tatttattca tctccttacg cactatattc
      241 agagcaataa gttgcctctt ttcatcttga ttcagcacaa cctttgcaac taaaaggagc
      301 ccatgatggt tacagttgtc agcaattatt gtcaattatc tcaaacccaa ctcagtcaga
      361 catttgcaga aaaatttact gtgaccgagg aattactgca gtctttaaaa aaaacagcgt
      421 tatccggaga tgaagaaagt attgagttac tgcataatat tgcgttaggt tatgatgaat
      481 ttggaaaaaa agctgaagat attctttacc atattgttag aaacccaaca aatgagaccc
      541 tatcga
//
LOCUS       DBKEKI010000397          543 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.405, whole genome shotgun sequence.
ACCESSION   DBKEKI010000397 DBKEKI010000000
VERSION     DBKEKI010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.405"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..500
                     /locus_tag="ACUQPX_004559"
     CDS             <1..500
                     /locus_tag="ACUQPX_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="proton-motive-force-driven choline transporter;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="high-affinity choline transporter BetT"
                     /protein_id="HHV9058951.1"
                     /translation="DTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAME
                     EVAQELRLRGAYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYR
                     ARSGKSTYYRLETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSV
                     MFPDA"
BASE COUNT          127 a          136 c          163 g          117 t
ORIGIN      
        1 gcgataccgc gccgcgaccg ctggggcttc aggatcgcct gagctggaaa aaacgtctct
       61 cgcgcctgat gaattatccg ggcacgcgtt acactaaaca gatgatggag acggtctgtt
      121 acccggcaat ggaagaagtg gcgcaggagt tgcggttgcg cggcgcgtac gtggagctaa
      181 aaagcctgcc gccggaagag ggacaacagt tggggcatct ggatttgttg gtgcatatgg
      241 gcgaagagca aaactttgtc tatcagattt ggccgcagca atattcggtg ccgggcttta
      301 cctaccgcgc acgtagcggt aaatcgactt actaccggct ggaaactttc ctgttagaag
      361 gcagccaggg caacgacctg atggactaca gcaaagagca ggtgatcacc gatattcttg
      421 accagtacga gcggcacctt aactttattc atctccatcg tgaagcgccg ggccatagcg
      481 tgatgttccc ggacgcgtga ttgtttttgc ataaaaccgc gccatgtctg catatggcgc
      541 ggg
//
LOCUS       DBKEKI010000398          542 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.406, whole genome shotgun sequence.
ACCESSION   DBKEKI010000398 DBKEKI010000000
VERSION     DBKEKI010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.406"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..332)
                     /locus_tag="ACUQPX_004560"
     CDS             complement(<1..332)
                     /locus_tag="ACUQPX_004560"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="4-deoxy-L-threo-5-hexosulose-uronate
                     ketol-isomerase; catalyzes the interconversion of
                     4-deoxy-L-threo-5-hexosulose uronate to
                     3-deoxy-D-glycero-2,5-hexodiulosonate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HHV9058952.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGA"
BASE COUNT          164 a          121 c          115 g          142 t
ORIGIN      
        1 tttgcacctt taccaacata cagggcgtcg cggtgaccga tttcatagca ttgaccatcg
       61 acagtaatcg taccggcacc gccaatattg ataacaccta actcgcgacg ttcaaggaaa
      121 tagcttacgc cgagttgttt accaacttcc ccgccaacgg aaaccgtttt agttatcggc
      181 ataatgccgc caacaataat tcggtcaatg tggctgtaaa ccatggtgta ctcatcggcg
      241 acaaatacct tttcaaccaa aaattcattg cgcagccctt gggtatccag cgtttttgcg
      301 tgcgcaccgt ggatgctctg tcttacgtcc acatcaacct ccgataatca gtgaagcgtg
      361 aaaatagaaa acgatagtga aacgctgttt cgttttctct ataaagggat gatatgccca
      421 tgggctaggg ttttcaatta aaaataaaac aatgtttcat ttttaagttg aggattaaaa
      481 aaagtgcagt gtcgatcacg aatatgtcat gagaatggaa aaatgaggga gcccagaaaa
      541 tg
//
LOCUS       DBKEKI010000399          539 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.407, whole genome shotgun sequence.
ACCESSION   DBKEKI010000399 DBKEKI010000000
VERSION     DBKEKI010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.407"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..207
                     /locus_tag="ACUQPX_004561"
     CDS             <1..207
                     /locus_tag="ACUQPX_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHV9058953.1"
                     /translation="VYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHM
                     DLYDGKAYVAEAISVLAPFFEETL"
     gene            207..>539
                     /locus_tag="ACUQPX_004562"
     CDS             207..>539
                     /locus_tag="ACUQPX_004562"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHV9058954.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGV"
BASE COUNT          144 a          112 c          143 g          140 t
ORIGIN      
        1 gtgtacctga ctcagccgat gcagattgtg gcgggcagcc aggcagggag caaatggatg
       61 agtgacgacc tgtacgatcg ggcatcaagc caggataaac gctatcacat tgttgaaggc
      121 gcaaaccata tggatcttta tgacggcaaa gcctatgtgg ctgaagcaat ttcagtatta
      181 gctccgttct ttgaggaaac tctgtaatga aacatattca aatccgcaat agtgatatgg
      241 cctggcatat tgctgccaat attcagtttc caccaaattt tgatgagtca aaacagtatc
      301 cggcgattat cagcgtccat ccttttggca gttgtaagga gcagacgtct ggcaatatct
      361 atggcaaggc attggctgaa aaaggctatg tcgttctcgc ttatgacgcc agtttccagg
      421 gagaatctgg tggggagcct cgttggatag aagatccgac acagcgcgta gaggatatta
      481 gccgtgttat cgattatgca gtaactctgc catatgttga tgctgaacgc attggcgtg
//
LOCUS       DBKEKI010000400          531 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.408, whole genome shotgun sequence.
ACCESSION   DBKEKI010000400 DBKEKI010000000
VERSION     DBKEKI010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.408"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..333)
                     /gene="dosC"
                     /locus_tag="ACUQPX_004563"
     CDS             complement(<1..333)
                     /gene="dosC"
                     /locus_tag="ACUQPX_004563"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HHV9058955.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMG"
BASE COUNT          156 a          129 c          104 g          142 t
ORIGIN      
        1 ccccatctcg acaatttcta ccggaattcc tatgcgggca tgcacttccg cgacggtatg
       61 ctggatttgt attagccttt cgacatcgtc aacctgggca gaaagcacgt taataatcca
      121 gcgttccatc gcacttttca actgccgctc aacttgttca ttactcaaga attcttcggc
      181 atgcgggtcg atgcggacaa ttcgataaaa ctcgatactc agataatgag catgcgcaag
      241 ggcaatttct gcggctttag cacgaatgag aggatctgcc tgttcgacaa gtccggtcca
      301 ctcatctttc attcttttaa aatacatctc cataattcac acccttataa ggctgggaaa
      361 tcagacggaa taaaaacgaa acgcaacgtg cgagatcgac taactgcacc atattctcct
      421 gaaatatgaa gatatactga aaagaaataa gcgacttaag acagtttcaa tctacgctac
      481 tgttcttcag aagagtatag cccatcgtaa ttatttttcg gtgacagcga a
//
LOCUS       DBKEKI010000401          531 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.409, whole genome shotgun sequence.
ACCESSION   DBKEKI010000401 DBKEKI010000000
VERSION     DBKEKI010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.409"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..>531)
                     /locus_tag="ACUQPX_004564"
     CDS             complement(<1..>531)
                     /locus_tag="ACUQPX_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HHV9058956.1"
                     /translation="LLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLL
                     NPIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPA
                     KTSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLP
                     YLLLGVLIGSFIYGFI"
BASE COUNT          170 a          113 c          119 g          129 t
ORIGIN      
        1 gaataaagcc ataaataaaa gatcctatta atactcctaa caaaagatat ggcagtacat
       61 ctttaaactg ttgcaacgca tctttggtgg ctagttttat gggattaata cttactacag
      121 ctttggtact gcaacaggta actacggggt tctcaatggc tttagccgct ggactacaac
      181 aactcacttt tatgggcgta tatgtcgttc ccggcgaagt ttttgctgga gcacaacaat
      241 ttgctgatga gcttttactg gcaatgatat gacgttcaaa ccccagagaa tctaggataa
      301 tactggcaag aacggagacg ccggctgcga taatcgcgta caacaaggtg actttccaac
      361 caaaggtcat ccacattagt ccgacgataa tgggattaag taatggtgaa acaaataaaa
      421 aagtgagagt cggaccaaac ccggctttcg ctgacaacaa tccacgtaac atgggtattg
      481 tcgagcaact acagaacggg gtaacggctc ccaacagagc agccaggaga t
//
LOCUS       DBKEKI010000402          527 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.410, whole genome shotgun sequence.
ACCESSION   DBKEKI010000402 DBKEKI010000000
VERSION     DBKEKI010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.410"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..304
                     /locus_tag="ACUQPX_004565"
     CDS             <1..304
                     /locus_tag="ACUQPX_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HHV9058957.1"
                     /translation="PSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTK
                     GMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          149 a          116 c          113 g          149 t
ORIGIN      
        1 tccctcagcg tggattgccg ctcatgcagg agcagataat cccttcgcca tcccattaag
       61 cgccgttgtc ggtattccgc tgtatatccg ggctgaggcg gttatccctc tggcatctgt
      121 tctgatgaca aaaggaatgg gtctgggagc attaatggca ttaatcatcg gcagtgccgg
      181 cgcaagcctg acggaagtga tattacttaa atcaatgttc agaatgccga tgatagctgc
      241 attcctgacg gttatattag gtatggctat cttgatgggc tatttgactc aattgctatt
      301 ttaaatagat gccagcagta aactataaga tgcatagtta gtgccatatc cttctaagta
      361 gaaaatcata gacaggataa tccttaaaaa tcacaaatga tttaactaat gcattgagaa
      421 tgatgacggc tcggtcaaca agtcatcaac agacaataac cacgtaatca tgcttctctg
      481 acattttatc ggagaagcaa tgactctttg tctcagtacc ctcctcc
//
LOCUS       DBKEKI010000403          514 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.411, whole genome shotgun sequence.
ACCESSION   DBKEKI010000403 DBKEKI010000000
VERSION     DBKEKI010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.411"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            245..472
                     /locus_tag="ACUQPX_004566"
     CDS             245..472
                     /locus_tag="ACUQPX_004566"
                     /inference="COORDINATES: protein motif:HMM:NF025073.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9058958.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
BASE COUNT          154 a          104 c          111 g          145 t
ORIGIN      
        1 gcgttccttg aaacgcatta tttccgaggg cataaaaatg cgctctgcat ttttgctttc
       61 tttttcacct gtcatgctca atctctctgt tatataaaga attttaaaga attctttgat
      121 ttgcattggt taaaataaaa atgcgcatag ctcttgaaat gcgcattttt ctgcgctatc
      181 gtttttcccg atagcggtag ccggtatcag taaccgaaaa cgttaagaga gtaaggatcg
      241 cagaatgtca gcaaaaaacc aagactggca cgcagagcag ataaaggctg ctgttcgtat
      301 gaaggggaag acactatctc aattatccag agaggcaggc ttaaagcctg acactatgcg
      361 taacgtattt cgctgccata taccacgata cgaagcaatc attgctagat atttggatat
      421 ggaaccagcc cagatttggc cttctcggta tatggagaaa acatcatgtt aacccattgg
      481 gtaacagcgc aagaatgcgt aggaatgtca tgct
//
LOCUS       DBKEKI010000404          513 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.412, whole genome shotgun sequence.
ACCESSION   DBKEKI010000404 DBKEKI010000000
VERSION     DBKEKI010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.412"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..278
                     /locus_tag="ACUQPX_004567"
                     /pseudo
     CDS             1..278
                     /locus_tag="ACUQPX_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(353..428)
                     /locus_tag="ACUQPX_004568"
                     /pseudo
     CDS             complement(353..428)
                     /locus_tag="ACUQPX_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          123 a          114 c          139 g          137 t
ORIGIN      
        1 gcctgaacgt gtactgtcgt aatggctagg tggaaatcga caacaacatc ggtgaaaacg
       61 cgctgcgatc ggtggcggtt ggaagaaaaa attatctctt cttcggttct gacaacggtg
      121 gagaaagcgc ggcgattatc tacagtctgc tggtcacctg caaactgaac ggagtggagc
      181 cggaggactg gttgcgcgaa gtgatcgtga agctcaatga ctggccgtcg aaccgagtgc
      241 atgaactgct gccctggaac ttctcagctg taaaataatc tttccgctac gtacttctct
      301 gggcgcttac cactcaacgg catcgtcaaa attatcaaag cgcagaacat agatttatca
      361 tgtgttttat ggatggcttt ctccatcagg catcgttcgt cacgggaaat tggtgctatg
      421 atcggcattg ctcagtccgg ttggtgattt gttttgattt ggcgattgat cagatcgcac
      481 aatccgggct gagttccctc taagtgatct agg
//
LOCUS       DBKEKI010000405          511 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.413, whole genome shotgun sequence.
ACCESSION   DBKEKI010000405 DBKEKI010000000
VERSION     DBKEKI010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.413"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..511)
                     /locus_tag="ACUQPX_004569"
                     /pseudo
     CDS             complement(1..511)
                     /locus_tag="ACUQPX_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT          158 a          144 c          107 g          102 t
ORIGIN      
        1 cagtaatcgc aggcgcggtt accgccatcg ccacatacag gagcgacata acaaacatcg
       61 caatccctgc cacagaaccg acgattttca gcgatttcat accgcgtgaa gcaacccaca
      121 taaagaagat aaacagcacc agcgttaacc cctgtaacgc tacgactgaa tattctttga
      181 ttagcgaacc gtcgcctttc atcgcccaac cgagcgcaat cagaattgcc tagggttttt
      241 gtgccagata gggaatatgc accacccagt aggtccacgc ggcgagataa gccagtccgg
      301 gtcccatcgt gtgtttaatc caggtactga ccccgccctt cccatctttg aaggtcgagc
      361 ctaactgacc aacaattagc gcataaggtg tgaaatagag tgcaaagata aacacccatg
      421 aaaaaacaac caccagcccc tggttggcat agttattaac aacgttgcca aaaccccaga
      481 cagtgataaa agacatcagc gcaatgttgt a
//
LOCUS       DBKEKI010000406          511 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.414, whole genome shotgun sequence.
ACCESSION   DBKEKI010000406 DBKEKI010000000
VERSION     DBKEKI010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.414"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..>511)
                     /locus_tag="ACUQPX_004570"
     CDS             complement(<1..>511)
                     /locus_tag="ACUQPX_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HHV9058959.1"
                     /translation="ADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFANN
                     TVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAM
                     FRNNIANDGKGGAIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKH
                     PSGYTIINNT"
BASE COUNT          143 a          115 c           98 g          155 t
ORIGIN      
        1 cggtgttatt tattatcgta taacctgaag gatgcttgct gtcgctatta ttatcggtaa
       61 catcgattgc cccgccatcg ccatcactgt aacttgttga tgtatatgct tggttattgt
      121 taaaaacatc atcacttaaa tagacgtcat tattaatggt ataaattgcg ccacctttgc
      181 catcattagc gatattattg cgaaacatgg cgttagtgac acgtaaatcg acggcaccag
      241 tatcattagt accagaagaa tagattgcgc caccatagcc gcctgcgacg ttaccggaaa
      301 aaataacatc agtaagattc agcgttgagt tttctttagc aaatatggcc ccgccgttat
      361 tatattctcc tgtaacggtg ttattggcaa acagagtcat tccagtttca ttttctggta
      421 atagtgaaaa ttccgctcct tgttgcaaaa atacggcccc accgctggca ttattggtca
      481 tatccgaaaa aaccagccat tgcccatcgg c
//
LOCUS       DBKEKI010000407          508 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.415, whole genome shotgun sequence.
ACCESSION   DBKEKI010000407 DBKEKI010000000
VERSION     DBKEKI010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.415"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            47..>508
                     /locus_tag="ACUQPX_004571"
     CDS             47..>508
                     /locus_tag="ACUQPX_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HHV9058960.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYL"
BASE COUNT          137 a          129 c          135 g          107 t
ORIGIN      
        1 cataaaacac tatcagtaag ttggagtcat taccgaggaa agcatcatgc cactcttaaa
       61 gctctgggca ggttcactgg ttatgttggc agccgtgagc ctgccgctac aggcggcttc
      121 ccccgttaaa gtcggttcaa aaatcgatac cgaaggcgcg ctgctcggca acatcatctt
      181 gcaggtactc gaaagccacg gcgtaccgac ggtgaataaa gtgcaactcg gaacgactcc
      241 tgtggtgcgc ggggcgatta cttccggtga actggatatc tatccggaat ataccggcaa
      301 tggcgctttc ttctttaaag atgaaaacga tgcagcgtgg aaaaatgccg ggcagggcta
      361 cgagaaagtc aaaaagctcg atgcggagca caacaagtta atctggctga cgcccgcgcc
      421 tgcaaataac acctggacta tcgccgtgcg tcaggatgtg gcagagaaaa acaaactcac
      481 ttcgcttgcc gacctgagtc gttatctg
//
LOCUS       DBKEKI010000408          499 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.416, whole genome shotgun sequence.
ACCESSION   DBKEKI010000408 DBKEKI010000000
VERSION     DBKEKI010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.416"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..416
                     /locus_tag="ACUQPX_004572"
     CDS             <1..416
                     /locus_tag="ACUQPX_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9058961.1"
                     /translation="CELNAGQIVEFDFGDIGASLFSAAGPGNRPAGVMPQTKSIAVKC
                     TNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITPNDLNSVIPFRLDAA
                     AAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
     gene            427..>499
                     /locus_tag="ACUQPX_004573"
     CDS             427..>499
                     /locus_tag="ACUQPX_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HHV9058962.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPL"
BASE COUNT          112 a          111 c          143 g          133 t
ORIGIN      
        1 actgcgaatt aaatgccggg cagattgttg aatttgattt tggtgatatc ggcgcatcgt
       61 tatttagtgc ggcagggccg gggaatcgac cagctggcgt catgccgcaa accaagagca
      121 ttgcggtcaa atgtacgaat gttgctgcgc aggcttattt aacaatgcgt ctggaagcca
      181 gtgccgtttc tggtcaggcg atggtgtcgg acaatcagga tttaggtttt attgtcgccg
      241 atcagaacga tacgccgatc acgcctaacg atctcaatag cgttattcct ttccgtctgg
      301 atgcagctgc ggcagccaat gtcacacttc gcgcctggcc tatcagtatt accggtcaaa
      361 aaccgaccga agggccgttt agcgcgctgg ggtatttacg cgtcgattat caatgaggta
      421 cggagaatga gaagagtatt ctttagctgt ttctgcgggt tactgtggag ttccagtgga
      481 tgggcagctg accctttag
//
LOCUS       DBKEKI010000409          483 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.417, whole genome shotgun sequence.
ACCESSION   DBKEKI010000409 DBKEKI010000000
VERSION     DBKEKI010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.417"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>483
                     /locus_tag="ACUQPX_004574"
     CDS             <1..>483
                     /locus_tag="ACUQPX_004574"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="RNase BN; member of metallo-beta-lactamase family;
                     the purified enzyme from Escherichia coli forms dimeric
                     zinc phosphodiesterase; in Bacillus subtilis this protein
                     is a 3'-tRNA processing endoribonuclease and is essential
                     while in Escherichia coli it is not; associates with two
                     zinc ions; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HHV9058963.1"
                     /translation="GPQGIREFVETALRISGSWTDYPLEIVEIGAGEILDDGLRKVTA
                     YPLEHPLECYGYRIEEHDKPGALNAQALKAAGVPPGPLFQELKAGKTITLEDGRQING
                     ADYLAAPVPGKALAIFGDTGPCDAALDLAKGVDVMVHEATLDITMEAKANSRGHSSTR
                     "
BASE COUNT          125 a          121 c          133 g          104 t
ORIGIN      
        1 atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc ggctcatgga
       61 ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat gatggcctgc
      121 gcaaagtaac cgcttatccg ctggaacacc cactggaatg ttatggctat cgtattgaag
      181 aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc gtgccgcctg
      241 gcccactgtt tcaggaatta aaagcgggca aaacaatcac gctggaagat ggaaggcaga
      301 ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct attttcggcg
      361 ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc atggtgcacg
      421 aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat agctctacac
      481 gcc
//
LOCUS       DBKEKI010000410          477 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.418, whole genome shotgun sequence.
ACCESSION   DBKEKI010000410 DBKEKI010000000
VERSION     DBKEKI010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.418"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..315)
                     /locus_tag="ACUQPX_004575"
     CDS             complement(<1..315)
                     /locus_tag="ACUQPX_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HHV9058964.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCI"
     gene            complement(332..>477)
                     /locus_tag="ACUQPX_004576"
     CDS             complement(332..>477)
                     /locus_tag="ACUQPX_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HHV9058965.1"
                     /translation="SLTQEGDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITY
                     RFG"
BASE COUNT          133 a          112 c          108 g          124 t
ORIGIN      
        1 aatacagaaa aaaacttccg ctcttgccgc ttttagcatc gatgctttgg tgctgataat
       61 tccatccgcc tcggtcacca gtttcaaaaa ctgaatcacc acttctttat ttgacgcgcc
      121 ttccagcaaa tcaacatgaa taaaagcata tttcccggcg ttcttaatct ttttaacgat
      181 attactgatg gtgcagatat tgccgtacaa cacggaaata aactggcatt cagaatcgat
      241 ggccagttgc aggctggcat tatctttaac ggcagcaatc accggattct ggcggagcag
      301 gtgtaagagg ggcataaatt atccttatta tttatccaaa gcggtaggtg atgccgaatc
      361 cggaaggcgg atagcgccac tgttgtagtg tggattcgtc gcacagcaaa cggcaggtgc
      421 cacactccag gcagccgcga tagtcaatac gtaagtcacc ttcctgtgtg agagaaa
//
LOCUS       DBKEKI010000411          476 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.419, whole genome shotgun sequence.
ACCESSION   DBKEKI010000411 DBKEKI010000000
VERSION     DBKEKI010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.419"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..338
                     /locus_tag="ACUQPX_004577"
     CDS             <1..338
                     /locus_tag="ACUQPX_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058966.1"
                     /translation="IYFDKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYA
                     FHHALSDSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNAC
                     RAAMLKGDK"
     gene            338..>476
                     /locus_tag="ACUQPX_004578"
     CDS             338..>476
                     /locus_tag="ACUQPX_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058967.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPLGDMVEIVEMAMRKAGN
                     SP"
BASE COUNT          135 a          104 c          121 g          116 t
ORIGIN      
        1 ctatatattt tgacaaagcg gaagttgagc gttgggtacg actgcataaa gcatgtgatt
       61 ttcgggcaga tataacaccg ctttacgcag cgccggcagt gccggttgca gtaaacgacg
      121 acatggctta cgcattccat cacgcactgt cagattcatc gcttggctct gatgaaatcg
      181 aagaaattaa aaccggtttg cgtgctgcct ttgccaacgt cactatccaa ccagagccga
      241 tagtgccgga tgaaatcggg ccaaacgata gtaatacgtt tgattatgtt gatggctgga
      301 acgcctgccg cgctgccatg cttaagggag ataaataatg attaatcgaa ccaaactgga
      361 gcacattctc gaatatgcca ggcagcagaa atgtattggg caactttgta aaattccact
      421 aggagatatg gttgaaatcg tggaaatggc catgcgtaag gctggcaact ctccgg
//
LOCUS       DBKEKI010000412          472 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.420, whole genome shotgun sequence.
ACCESSION   DBKEKI010000412 DBKEKI010000000
VERSION     DBKEKI010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.420"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            207..254
                     /gene="ydaN"
                     /locus_tag="ACUQPX_004579"
     CDS             207..254
                     /gene="ydaN"
                     /locus_tag="ACUQPX_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HHV9058968.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            268..>472
                     /gene="zntB"
                     /locus_tag="ACUQPX_004580"
     CDS             268..>472
                     /gene="zntB"
                     /locus_tag="ACUQPX_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HHV9058969.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRD"
BASE COUNT          133 a           88 c          114 g          137 t
ORIGIN      
        1 tgtgcgtaat cattgagatc ccgttatttt tatcaatttt tgttgctatc cgatcgcaaa
       61 aaagccacgt catatgatca gataattctg ataatgatag acgctattta acacttcaca
      121 cggtttgtat acggaaaagc attttgcttt ttgtattcaa tttagacaga attttattaa
      181 tcatttcagg gtaatggggt gatgagatgt tgcgtaacag ggccagaagg ctagactaca
      241 aaataatgcg ttgatgatgg aggcactgtg gaagcgatta agggatcgga cgttaatgtc
      301 ccggatgcag tatttgcctg gatgctggat ggtagaggcg gcgttaaacc gctggaaaat
      361 acagatgtga ttgatgaagc gcatccctgc tggctccacc ttaattatgt acaccatgat
      421 agcgcccaat ggctggcgac aacaccgctg cttcccaata acgtacgtga tg
//
LOCUS       DBKEKI010000413          465 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.421, whole genome shotgun sequence.
ACCESSION   DBKEKI010000413 DBKEKI010000000
VERSION     DBKEKI010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.421"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(32..>465)
                     /locus_tag="ACUQPX_004581"
     CDS             complement(32..>465)
                     /locus_tag="ACUQPX_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HHV9058970.1"
                     /translation="YRAVDSRGRTVDFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFI
                     NTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMK
                     TAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM"
BASE COUNT          101 a          121 c          121 g          122 t
ORIGIN      
        1 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
       61 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
      121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
      181 tggcgccgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
      241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
      301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
      361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
      421 gataaaaatc gacagtgcgg ccccggctgt cgacggcccg gtaca
//
LOCUS       DBKEKI010000414          462 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.422, whole genome shotgun sequence.
ACCESSION   DBKEKI010000414 DBKEKI010000000
VERSION     DBKEKI010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.422"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            59..>462
                     /locus_tag="ACUQPX_004582"
     CDS             59..>462
                     /locus_tag="ACUQPX_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HHV9058971.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKSSEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQ"
BASE COUNT          134 a          112 c          129 g           87 t
ORIGIN      
        1 ctattttgca atcaatgcaa agtgcgggat gtctgcgggc aaaactggaa atcatctgat
       61 gatcagcgac agcaaactac tggcaagtgc ggcctgggat gcgcaatcac tcaacgaact
      121 aaaggcgaaa tcgagcgaag atccggcggc aaatatccgt ccggtggccc gtcaggtgga
      181 agggatgttc gtgcagatga tgttgaaaag catgcgcgac gctttaccaa aagatggcct
      241 gttcagcagc gagcacactc gtctgtatac cagtatgtat gaccagcaaa ttgcccaaca
      301 gatgacggcg ggcaaaggtc tggggcttgc agagatgatg gttaaacaga tgacgccaga
      361 acaaccattg ccagaggagt ccacgccagc agcaccgatg aaattcccgc tcgaaaccgt
      421 ggtgcgttat caaaaccaga cgctttcgca gctggtgcaa aa
//
LOCUS       DBKEKI010000415          458 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.denovo.423, whole genome shotgun sequence.
ACCESSION   DBKEKI010000415 DBKEKI010000000
VERSION     DBKEKI010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.denovo.423"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>458
                     /gene="dcuC"
                     /locus_tag="ACUQPX_004583"
     CDS             <1..>458
                     /gene="dcuC"
                     /locus_tag="ACUQPX_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HHV9058972.1"
                     /translation="LFPTLVSLGVSRLSAVAVIATTMSIEWGILETNSIFAAQVAGMK
                     IATYFFHYQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQAEQKTLDNVPPLYYAI
                     LPVMPLILMLGSLFLAHVGLMKSELHLVVVMLLSLTVTMFVEFFRKHNLR"
BASE COUNT          100 a          119 c          111 g          128 t
ORIGIN      
        1 ccttatttcc gacgctggtg agtctgggag taagtcgcct ctctgcggtg gcggttatcg
       61 caaccacgat gtccattgag tgggggattc tggaaacgaa ctccattttt gcagcccagg
      121 tcgcgggaat gaaaattgcc acatacttct tccactacca gcttccggtc gcctcttgcg
      181 tcattatctc ggtggcgatc tcccactttt tcgtgcaacg cgcttttgac aaaaaagata
      241 aaaatatcaa tcacgaacag gcagagcaaa aaactctcga taatgtcccg ccgctctatt
      301 acgccatttt acctgtgatg ccgttaatcc tgatgctcgg ctcgctgttc ctcgcccacg
      361 tcgggctgat gaagtcagaa ctgcatctgg tggtggtgat gttactaagt ttgacggtga
      421 cgatgtttgt tgagttcttc cgcaagcata acttgcgc
//
LOCUS       DBKEKI010000416        11314 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.guided.125, whole genome shotgun sequence.
ACCESSION   DBKEKI010000416 DBKEKI010000000
VERSION     DBKEKI010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.guided.125"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            215..1063
                     /locus_tag="ACUQPX_004584"
     CDS             215..1063
                     /locus_tag="ACUQPX_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HHV9058973.1"
                     /translation="MNQALPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKRNIIRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            complement(1149..1484)
                     /locus_tag="ACUQPX_004585"
     CDS             complement(1149..1484)
                     /locus_tag="ACUQPX_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HHV9058974.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRNTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            1717..2049
                     /gene="mobA"
                     /locus_tag="ACUQPX_004586"
     CDS             1717..2049
                     /gene="mobA"
                     /locus_tag="ACUQPX_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HHV9058975.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            2061..4772
                     /locus_tag="ACUQPX_004587"
     CDS             2061..4772
                     /locus_tag="ACUQPX_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HHV9058976.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRIRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVELGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPRMGFEPEGSYQQFNDVFAEMVAWHNAAGITGSGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            4840..5271
                     /locus_tag="ACUQPX_004588"
     CDS             4840..5271
                     /locus_tag="ACUQPX_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HHV9058977.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            6213..6452
                     /locus_tag="ACUQPX_004589"
     CDS             6213..6452
                     /locus_tag="ACUQPX_004589"
                     /inference="COORDINATES: protein motif:HMM:NF020425.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HHV9058978.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(6640..7617)
                     /gene="intI2"
                     /locus_tag="ACUQPX_004590"
                     /pseudo
     CDS             complement(6640..7617)
                     /gene="intI2"
                     /locus_tag="ACUQPX_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            7949..8422
                     /gene="dfrA1"
                     /locus_tag="ACUQPX_004591"
     CDS             7949..8422
                     /gene="dfrA1"
                     /locus_tag="ACUQPX_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HHV9058979.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            8517..9041
                     /gene="sat2"
                     /locus_tag="ACUQPX_004592"
     CDS             8517..9041
                     /gene="sat2"
                     /locus_tag="ACUQPX_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HHV9058980.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            9099..9934
                     /gene="aadA1"
                     /locus_tag="ACUQPX_004593"
                     /pseudo
     CDS             9099..9934
                     /gene="aadA1"
                     /locus_tag="ACUQPX_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            9963..10460
                     /locus_tag="ACUQPX_004594"
     CDS             9963..10460
                     /locus_tag="ACUQPX_004594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058981.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
BASE COUNT         3035 a         2491 c         2920 g         2868 t
ORIGIN      
        1 ggggagagat gtaccagcca caaaactttt tgccgctggc gtcaaaaact tttgcatctg
       61 cccccggaca actgccccgt gacgtggtga gcgtcccccg tcggaaaggt ccactgcggg
      121 gaaataccct aagacccgga cggcagaagg agcttcgctc ctccgggaaa tgacctggct
      181 gacccggcag tcgtgacgat gaggagacaa tgtgatgaat caggctttac ccactgctga
      241 cctgaatact gccggcacga cagatgttat tccgtctgtg gctatcgacc gcatcatcgc
      301 gcagcgtaac gaaggtattg cactgttcat gcaggcgatg gaatgcctgg cgacagcgcg
      361 caggattctg ctcgatgctt ccggtgacat ttttctctac gggtttgaag actgtgtgac
      421 tgactccgtt cgatgcatgg ataaaccgga agaagcgaaa aggaatatca tccgtcttgc
      481 cgaccggaaa atctgggacc gcctgatgac agatacgggc atgtacacct tcatgagttc
      541 atgccagcgt gatgagtgga acagccagct gatgagcgac acctgtcctg aaatcaccct
      601 ggacaatgta ctggcaacat tccgccatct gaatgccagc aagatgcaga cgtttgaaca
      661 gggactgatt gatgtctacc ggaaattgtc atgggattac agaaccaata atccctgccg
      721 tctgggcaag aaaatcatta ttgaaaacct gctgtaccgc tggagtaacg ggcgtgtgac
      781 gctggactgc agcggacggg aggcactgga tgacctggta cgtccgtttt atctgctgga
      841 ggggcgcaac gttcctgact tcaggaacag catcggggcg cagtatggtg aatttctcgg
      901 gaacggcgat aatgtcggta agctgttaga aggggaatat tttacggtgc gtggctacca
      961 gaaagggacc gtacatattg tctttaagcg ttctgacctt gttgaaaaac tgaatgatat
     1021 tattgcacgg cattatccag gtgtattgcc gccacgagtc tgattaaaca gaaaagccct
     1081 ggtatttatg ccggggcttt tttttctgta atcatcataa tctcagaaca cgatgtgaca
     1141 ttgtcacatc aattgttgtt catatcgatg ccatctttcc tgcctgaatc tgtcttttta
     1201 ggtgctgttt tagaggaaga acggcgggat ggttttttcg attctgcttc ccgaataatg
     1261 tcacgaagtg ctttctctcc tacttcatac cctgcatttt tgagcgtgtt acgaacatct
     1321 gccagtgtat aaccctttgt ccttaccagg acaataatgt catcacgaat ggcatcaaga
     1381 aaatcacgca atgttttccg ttgagcggtg agatctggca attccgataa agcagctttt
     1441 gccagctgga tgtcatcatc tgagtaaaat tttttagacg ccattggcac tttctctccg
     1501 tttaatattg aagcctgatt ttatcaccaa aaactgactc ctggcagggc tgcgccctgc
     1561 ctctgccgtc tgcataagac tatgatgcac aaaaataaca ggctataatg gcctgaaaaa
     1621 cgggacagga tgtgggaatg tcggaccgcg cacgcgcact cagacattcc ccacctggtc
     1681 agggctttgc cccgacaccc cggtaaggag cagaccatga gtgagaagaa aacccgaacg
     1741 ggcagtgaga acaggaagag aattgtaaaa ttcacggcga gattcacgga ggatgaggct
     1801 gaaatcgtaa gagaaaaagc ggagtcaagt ggacagacgg tttcaacatt tatacgaagt
     1861 tcttcactgg ataaggtggt taactgccgt actgacgacc gtatgattga tgagattatg
     1921 cgtcttggcc gccttcagaa gcatctgttt gtggagggaa aacgcactgg tgataaggag
     1981 tatgcagagg ttcttgttgc cattactgaa tatgtcaatg ctctgcgaag agacttaatg
     2041 ggacgctgag ataactgctg atgaatgcca ttattccgca caaaagacgt gacggcaggt
     2101 cttcctttga agacctgatt gcgtacacgt ctgtgcggga tgatgtgcag gaaaatgagc
     2161 tcacagaaga cagcgaaatc aagagtgatg tccctcaccg caaccgtttc agacggcttg
     2221 ttgattatgc aacacgcctg cgtaacgaaa aatttgtctc cctgattgat gtgatgaagg
     2281 acggcagtca gtgggtaaat ttctatggcg taacctgttt ccataactgc aattcactgg
     2341 aaacagctgc tgaagagatg cagtacaagg cggacaaagc tgttttttcc cgtaatgata
     2401 cagaccccgt tttccactac attctttcct ggcctgcgca tgaaagcccg cgtcctgagc
     2461 agctttttga ctgtgtgcgt cacacactga aatctcttga actctcaaaa catcagtacg
     2521 tggctgccgt tcatacggac acggataatc tgcatgtgca tgttgccgtg aatcgtgtac
     2581 atcctgaaac tggttatatt aactggcttt cctggagcca ggaaaaactc agccgtgcat
     2641 gtcgtgaact tgaacttaag cacggattcg cgcctgataa tggctgcttt gtgcatgcac
     2701 ccggcaaccg tattgtgcgc aaaacagcgc tggtacgtga gcgccgtaat gcctggcgac
     2761 gcgggaaaaa gcagacgttc agggaatata ttgcgcagat gtcgattgcc gggctgcgtg
     2821 aagagcccgc acaagactgg ttgtctttgc ataaacggct ggccagtgat ggtctgtata
     2881 tcacgatgca ggaaggggaa ctggtggtga aggatggctg ggacagagcg cgggaaggtg
     2941 ttgcactcag ttcgttcgga ccgtcatgga cagctgaaaa actggggcgg aaactggggg
     3001 aatatcagcc tgtaccgaca gatattttca gtcaggtggg gacacccggt cgttatgatc
     3061 cggaagccat aaacgttgat atccggccgg aaaaagtggc agaaacggag agtctgaaac
     3121 agtacgcctg ccgtcatttt gccgaacgct taccggaaat ggcgagaaat ggtgagctgg
     3181 aaagttgcct ggatgtgcac agaactctgg caaaggctgg attatggatg gggatccagc
     3241 acgggcatct ggtactgcat gatggatttg ataaacaaca gacaccagta cgtgcagaca
     3301 gtgtgtggcc gttaatgacg ctggattata tgcaggatct ggatggtgga tggcagcctg
     3361 taccgaagga tatttttact caggttatac ccggagaacg attccgggga cgtaatcttg
     3421 gtactcaggc tgtcagtgat tacgaatggt atcgtattcg gatggggaca ggtccacagg
     3481 gagcaataaa gcgcgaactg ttttccgata aggaaagtct gtgggggtac acgactgttc
     3541 agtgtgagtc gttgattgaa gatatgatcg cgggcgggaa tttcagctgg caggcgtgcc
     3601 atgagatgtt tgcgcgtaaa gggcttatgt tgcagaaaca gcatcatggt cttgtgattg
     3661 tggatgcgtt taatcatgag ctcacaccag tgaaggccag cagtatccac ccggatttga
     3721 cgttatccag ggctgagccg caggcagggc catttgagat tgcaggtgca gatatatttg
     3781 aacgggtgaa gcctgaatgt cgctacaatc cggaacttgc tgccagcgat gaagtggagc
     3841 tcggcttccg gcgcgatcct gtactgcgtc gtgaacgccg tgaagcccgt gcagctgcgc
     3901 gggaagattt gcgcgcgcgt tatcttgcat ggaaggagca ctggcgtaaa cccgacttac
     3961 gttacggaga acggttacgg gaaattcatg ctgcctgccg tcggcgcaag gcgtatatcc
     4021 gtgtgcagtt ccgggatcca caattgcgta agctgcatta ccatattgca gaagtgcagc
     4081 gtatgcaggc gctgatcagg ctgaaggaga gtgtgaagga agagcggttg tcactgattg
     4141 cggagggtaa gtggtatcct ttgtcctacc ggcagtgggt ggagcaacag gcggtacagg
     4201 gagacagagc tgcattatca cagttacgtg gctgggatta tcgtgatcgt cgcaaagaca
     4261 agcgacgaac aacgaatgcc gaccgctgcg tgattctctg tgaaccggga ggaactccac
     4321 tttatgaaga cactggcgtt ctcgaagccc gtctgcagaa agatggcagt gtgcgttttc
     4381 gtgatcggag gaatggcgag ttagtctgtg tggattatgg ggaccgtgtg gtgttctacc
     4441 atcatcagga cagaaatgaa ctggtggata agctgaatct gattgctcct gtattgtttg
     4501 atcgtgagcc aagaatgggt tttgaaccgg aaggctcata tcaacagttt aatgatgtat
     4561 ttgcggaaat ggtggcctgg cataatgcag ctggaataac cggaagtgga cactttgtaa
     4621 tcagccgccc ggatgtggat ttacatcgtc agcgtagtga acagtactac cacgaatata
     4681 tcaggcaaca gaaaagcata tccggggggc atggcgcatc ttatgctcca gttcaggata
     4741 atgagtggac gccaccatca cctggaatgt agggatgagg agtggatatt aaaaatctta
     4801 acttgtatta gttaagattt tctttgctct gacagagcta tggtactgag atctatatgg
     4861 attgaggagc aatatatggg ggtaaagaaa atcgttatgc tggctatttt agtcagcttt
     4921 gtggcgggat gtgcgccatt acatccttca aattgccata aaactacagc cctgggtagt
     4981 tgcagttcag gacgttggga tgatcaggat gaatgggggg agcaagcacg gggaatcagg
     5041 gatgcaatta atgctaaact tgatgatccg caaaaatgga aaaggaaaaa atgcaggttg
     5101 catatggaat tctcccggga tggcacggcg ttaaaaatat caaccagtaa tggcgataaa
     5161 gcctattgcg aagcgataaa gtcagcagcc cataaagcta aattcccggc cttcaacaat
     5221 ccggaagttt atagagattt tcaaaaatcc ggctttgaca tgcgcggtta gctccacgac
     5281 atatctgtag ggctaacaga agactgtcag gttggctgat tactacactg gaacctgagg
     5341 tactttgcct caggttatgt tgacatactc aaaaatagat tcttacatta tcccggtaac
     5401 ggcgtccttc atcggcctgc cagttaccat caatggtatt ggtaccctcg ttctttctgt
     5461 ctgtgatgtt aataagggca aaattaatca gcatgtctgg caggaatgat tccgcgtctg
     5521 gcacagccag gcacctgatg gcatgaaatg ccgctaagcc cgcgtaattg cgggcttttt
     5581 tttgtttgtg tttggcacga tctggcaacg ggaggaagcg ccaagcaccg tttgagcatg
     5641 gcattaaagc tggtattatg gtttggcgat gccatgattg atgccgatac catgctgcgt
     5701 tcttgtgggc ggacaaaata gttgggaact gggaggggtg gaaatggagt ttttaaggat
     5761 tatttaggga agagtgacaa aatagatggg aactgggtgt agcgtcgtaa gctaatacga
     5821 aaattaaaaa tgacaaaata gtttggaact agatttcact tatctggtta actaatctag
     5881 ctaacctgcc gctcagctta gtacgatcct atatccaact gatcataatc ctttcatcag
     5941 tatcccaaat taaatccccc tcctagactg cctcaccaac actttacagt gatcccgtat
     6001 ttccggacag gtgattgatt tttttcgctt gtacaggtgt taagccacag ttatcttgat
     6061 gtggacatta tgcattgtaa tagcccatta acatgcgcta agagcataaa ttaatataca
     6121 gtaacaagca ttacgaggtg ctaatgtttg gaaagcagcc tcttccaact acaataataa
     6181 gttaactatc ctccacccac aaataattac aaatgacccc tttccgctat aactccgatc
     6241 tcaccagcgg ctcgctacaa acccgagagt gtcgcattat tactggcttg ttactccaag
     6301 agcttgatga agctgcctgg gataaagcca tgtataagga aaatgtgctt cagaaacgca
     6361 cgcaatctac ggtaagacgt atttcttcag cgcttagaaa acgcctagaa cacctaagct
     6421 ctgacttttg ggcttttgcg tttttatgtt aggcattgtc tggcaactgg aggaagtgta
     6481 atgaccatga atggtcactg tgattgacag tatcttgctt aaacttatct gaacaatggt
     6541 agaactggct catatttatc ccctctatga ttgtttatct ttttcaccat aaaaagtatc
     6601 aggggctact cagggctaca taaagatatt tatctacttt tactgattga taagtagcat
     6661 cagtccatcc gcaggactgg tggtgccggc aaaatgctga cccaacacat gcgtatagat
     6721 ttgcgtggtc ttaacatcgt tatgccctaa gagttcttgc acagtgcgaa tatcacgccc
     6781 cgcttgtaat agatgcgtag caaacgagtg acgaaatgta tgacaagtga cacgcttgct
     6841 aacgatgcct gctttttgta cggctgcctt caatgccttt cgcgcaacgg agtcatgcag
     6901 atgatggcgg cataatttgc cgttatacgg gtggttgcag agcgtgctgg agggaaagac
     6961 aaacatccac gccgcttgtc gataagcaga agggtatttg tgatctaaag caaaaggcag
     7021 cgatggccct acgccttgta agttgtcgtc ttgctgaata agccgcgctt gctcaatgag
     7081 ttattttatt gctgggatta ggcgcgtggg cagtaggctg tttctgcttt tcccaccctt
     7141 accgtcatgc acagtgatgc agccattatc aaaatcaaaa tctttaaccc gcaaacgcaa
     7201 gcattcatta atgcgcaaac ctgcaccata cagcagcgta aaaataactt ggttgcgagt
     7261 atccataacc tgcaaaatgc gttgcacttc atttgcagag ataacagagg gtagccgtct
     7321 aggcttgctt gcagggatat aatcaatatc gcccaacggc tgttgtaaaa acctgttgta
     7381 caaaaaagct agggcattta aagcgatttt ctgcgtgttt atggctacat gtctgctgtt
     7441 tgctaagctg gataaaaaca gcctgacctc ttcactgccc atggtctgag gatgacgttt
     7501 tttgtgaaac agaataaaac gcttaatcca gtgcaggtaa gttttttcag ttttcagcgc
     7561 ataacctttt tgtcgcatat ccgtgcgtat agaatttaaa aatggactgt tagacataaa
     7621 acgctccttg tcttgcaact gtctgcctat acagcctatt ctagctggga tttaaaaaag
     7681 tgcctgtttt ttacgcctag agatgcttgt ttaccggtag agttttaatt taatgctaaa
     7741 taaattaaaa tgttatgagt tctttgggtg agataatgtg catcgtgcaa gcaggataga
     7801 cggcatgcac gatttgtaat aacagagtgt cttgtatttt taaagaaagt ctatttaata
     7861 caagtgatta tattaattaa cggtaagcat cagcgggtga caaaacgagc atgcttacta
     7921 ataaaatgtt aacctctgag gaagaattgt gaaactatca ctaatggtag ctatatcgaa
     7981 gaatggagtt atcgggaatg gccctgatat tccatggagt gccaaaggtg aacagctcct
     8041 gtttaaagct attacctata accaatggct gttggttgga cgcaagactt ttgaatcaat
     8101 gggagcatta cccaaccgaa agtatgcggt cgtaacacgt tcaagtttta catctgacaa
     8161 tgagaacgta ttgatctttc catcaattaa agatgcttta accaacctaa agaaaataac
     8221 ggatcatgtc attgtttcag gtggtgggga gatatacaaa agcctgatcg atcaagtaga
     8281 tacactacat atatctacaa tagacatcga gccggaaggt gatgtttact ttcctgaaat
     8341 ccccagcaat tttaggccag tttttaccca agacttcgcc tctaacataa attatagtta
     8401 ccaaatctgg caaaagggtt aacaagtggc agcaacggat tcgcaaacct gtcacgcctt
     8461 ttgtaccaaa agccgcgcca ggtttgcgat ccgctgtgcc aggcgttagg cgtcatatga
     8521 agatttcggt gatccctgag caggtggcgg aaacattgga tgctgagaac catttcattg
     8581 ttcgtgaagt gttcgatgtg cacctatccg accaaggctt tgaactatct accagaagtg
     8641 tgagccccta ccggaaggat tacatctcgg atgatgactc tgatgaagac tctgcttgct
     8701 atggcgcatt catcgaccaa gagcttgtcg ggaagattga actcaactca acatggaacg
     8761 atctagcctc tatcgaacac attgttgtgt cgcacacgca ccgaggcaaa ggagtcgcgc
     8821 acagtctcat cgaatttgcg aaaaagtggg cactaagcag acagctcctt ggcatacgat
     8881 tagagacaca aacgaacaat gtacctgcct gcaatttgta cgcaaaatgt ggctttactc
     8941 tcggcggcat tgacctgttc acgtataaaa ctagacctca agtctcgaac gaaacagcga
     9001 tgtactggta ctggttctcg ggagcacagg atgacgccta acaattcatt caagccgaca
     9061 ccgcttcgcg gcgcggctta attcaggagt taaacatcat gagggaagcg gtgatcgccg
     9121 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     9181 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     9241 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     9301 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     9361 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     9421 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     9481 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     9541 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     9601 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     9661 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     9721 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     9781 ggcaggctta tcttggacaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     9841 ttgttcacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc
     9901 gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagagag ctggggaaga
     9961 ctatgcgcga tctgttgaag gtggttctaa gcctcgtact tgcgatggca tcggggcagg
    10021 cacttgctga cctgccaatt gttttagtgg atgaagctcg tcttccctat gactactccc
    10081 catccaacta cgacatttct ccaagcaact acgacaactc cataagcaat tacgacaata
    10141 gtccatcaaa ttacgacaac tctgagagca actacgataa tagttcatcc aattacgaca
    10201 atagtcgcaa cggaaatcgt aggcttatat atagcgcaaa tgggtctcgc actttcgccg
    10261 gctactacgt cattgccaac aatgggacaa cgaacttctt ttccacatct ggcaaaagga
    10321 tgttctacac cccaaaaggg gggcgcggcg tctatggcgg caaagatggg agcttctgcg
    10381 gggcattggt cgtcataaat ggccaatttt cgcttgccct gacagataac ggcctgaaga
    10441 tcatgtatct aagcaactag cctgctctct aataaaatgt taggcctcaa catctagtcg
    10501 caagctgagg ggaaccacta gtgtcatacg aacctccaag agacggttac acaaacgggt
    10561 acattgttga tgtcatgtat gacaatcgcc caagtaagta tccagctgtg ttcagaacgt
    10621 acgtccgaat tccagactat gtcgaactag ataacgcgga ggagctggcc gacgcaatca
    10681 ttcgagaggt tgagttaaag gaggcatggc tcacaagggt cttggggaac tcagccgatc
    10741 ctgacgcttc tgtgggcaag tgggaccggc gcctagcgca gtgcggtggt attgatgaga
    10801 tgtcaagtgg ggtgcaggca acatacttca gtccaaacgg tcagctggtc ggcttagtgg
    10861 catacattga tgccgaatgg atggatgaat agctctgacg ttacgattca ggtgagacaa
    10921 ccagatgggc attgcgtaca agctcagttg ccaccgatgt ggctacgagt ctgatcttct
    10981 ttacctaggc caaggtatgg cgatgcttcc tgagcaaata gtcggaacgt gcacatgctg
    11041 cgacaatcta acgacgattt ctataaatga aagctacgga gtatgctcac tttgtggggc
    11101 taaggatcgt gtgatattta caagctatca gcatgaggca aggccaagaa atccctgcta
    11161 tctagagaga agtttcaaat atcaatgccc aagatgccat gccttcagta tggagcctcc
    11221 ggatctaccg gagatgcttt gggactaatg ctccgctctg atgaatcccc taattgtcac
    11281 gaacggtgca atagtgatcc acacccaacg cctg
//
LOCUS       DBKEKI010000417         7809 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.guided.168, whole genome shotgun sequence.
ACCESSION   DBKEKI010000417 DBKEKI010000000
VERSION     DBKEKI010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.guided.168"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..603)
                     /locus_tag="ACUQPX_004595"
     CDS             complement(<1..603)
                     /locus_tag="ACUQPX_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HHV9058982.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGF"
     gene            865..1323
                     /gene="gpt"
                     /locus_tag="ACUQPX_004596"
     CDS             865..1323
                     /gene="gpt"
                     /locus_tag="ACUQPX_004596"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HHV9058983.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1415..2659
                     /gene="frsA"
                     /locus_tag="ACUQPX_004597"
     CDS             1415..2659
                     /gene="frsA"
                     /locus_tag="ACUQPX_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HHV9058984.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            2717..3118
                     /gene="crl"
                     /locus_tag="ACUQPX_004598"
     CDS             2717..3118
                     /gene="crl"
                     /locus_tag="ACUQPX_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HHV9058985.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(3157..4212)
                     /gene="phoE"
                     /locus_tag="ACUQPX_004599"
     CDS             complement(3157..4212)
                     /gene="phoE"
                     /locus_tag="ACUQPX_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HHV9058986.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            4500..5603
                     /gene="proB"
                     /locus_tag="ACUQPX_004600"
     CDS             4500..5603
                     /gene="proB"
                     /locus_tag="ACUQPX_004600"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HHV9058987.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            5615..6868
                     /gene="proA"
                     /locus_tag="ACUQPX_004601"
     CDS             5615..6868
                     /gene="proA"
                     /locus_tag="ACUQPX_004601"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HHV9058988.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            6983..7058
                     /locus_tag="ACUQPX_004602"
     tRNA            6983..7058
                     /locus_tag="ACUQPX_004602"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7016..7018,aa:Thr,seq:cgt)
     gene            complement(7073..7771)
                     /locus_tag="ACUQPX_004603"
                     /pseudo
     CDS             complement(7073..7771)
                     /locus_tag="ACUQPX_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         1885 a         1944 c         2002 g         1978 t
ORIGIN      
        1 aaaaccagct ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt cgataccccc
       61 cgcacaaccc atgtagattt caccttcttc ttcggagtcg gtgttaatca gaatatcagc
      121 ctgcaaccag ttgctctgta agccgaacgc accgtccata ccggcttctt cggtcatggt
      181 cagcagcact tccagcgggc cgtgaaccac gttttcgtca gccagaaccg ccagtgcaga
      241 ggccatacca atgccgttat ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc
      301 atcaatataa ggctggatag gatctttcgt gaagtcatgc acggtgtcgt tatttttctg
      361 cggcaccata tcgaggtggg cctgtaagac gaccggttta cgattttcca tacctgcggt
      421 agcaggttta cgaatcagga tattacctac ctgatcgcgt tcgacatgga aacctttctc
      481 ttttgcccaa ccaacaatgt attcagcgag ttgctcttca tgataggacg ggtgaggaat
      541 agaacagatt ttggcaaaaa tatcccacag cggctgtgga gataattgag acagttcaga
      601 cacgttaagt ctccttgtcg atcacccgca aaacagtatt gcaggtcaca gggttagcag
      661 aaaatgttgt caacacaaga caggcttgcg agatatgtat tgagaatacc actttatccc
      721 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
      781 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
      841 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
      901 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
      961 attgccgtaa gccgtggcgg tctggtaccg ggtgcgttac tggcgcgtga actgggtatt
     1021 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
     1081 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
     1141 accggtggta ctgctgttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
     1201 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
     1261 acctggatcg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
     1321 taatcttttc aacgcctggc attgccgggc gttgttcttt ttaacttcag gcaggttaca
     1381 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
     1441 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
     1501 cggcgcacaa ccgcctgtgc agtcggctct tgatggtaaa accatccctc actggtatcg
     1561 catgattaac cgtctgatgt ggatctggcg cggtattgac ccacgtgaaa tcctcgacgt
     1621 ccaggcacgt attgtgatga gcgatgctga acgtaccgac gatgatttat acgataccgt
     1681 gattggctac cgtggtggca actggattta tgagtgggcc acccaggcga tggtgtggca
     1741 acaaaaagcc tgtacggaag aagatccgca actcagtggt cgacactggc tgcatgcggc
     1801 tacgttgtac aacattgccg cctatcctca tctgaaagga gatgacctgg ccgagcaagc
     1861 gcaggcttta tcaaaccgcg cctatgaaga ggccgcccag cgtctgccgg gcacgatgcg
     1921 gcagatggag tttaccgtac ccggcggtgc gcccatcacc ggctttttgc atatgccgaa
     1981 aggcgatggc ccgttcccga cagtattaat gtgtggtggt ctggatgcga tgcagacgga
     2041 ctattacagc ctgtatgaac gttattttgc gccgcgcggc attgcgatgc tgactattga
     2101 tatgccgtcg gtgggctttt cttcaaaatg gaagctcacc caggactcca gcctgttgca
     2161 tcagcacgtc ttaaaggcgc tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc
     2221 ctttggtttc cgtttcggcg ctaacgttgc cgtgcgtctg gcataccttg aatcgccgcg
     2281 tctgaaagcg gttgcctgtc ttggtccggt agttcatacc ctgttgagtg attttaagtg
     2341 ccagcaacag gtgccggaaa tgtatcttga cgttctggcg agtcgtttgg ggatgcatga
     2401 tgcttccgac gaagcgttac gcgtggagct gaatcgctat tcattaaaag tgcaaggatt
     2461 gctggggcgt cgctgcccaa cgccaatgtt atcaggctac tggaagaacg atccgttcag
     2521 cccggaagag gactcacgct taatcacctc atcatctgct gacggtaaat tattagagat
     2581 cccatttaac ccggtgtatc ggaattttga caaaggtctt caggaaatca ccggctggat
     2641 cgaaaaacgc ttgtgttaaa aatttgctaa attttgccaa tttggtaaaa cagttgcatc
     2701 acaacaggag atagcaatga cgttaccgag tggacacccg aagagcagat tgatcaaaaa
     2761 atttaccgca ctaggcccgt atattcgtga aggtaagtgc gaagataatc gattcttttt
     2821 cgattgtctg gctgtatgcg tcaacgtgaa accggcaccg gaagtgcgtg aattctgggg
     2881 ctggtggatg gagctggaag cgcaggaatc ccgttttacc tacagctacc agtttggtct
     2941 gttcgataaa gcaggcgact ggaagagtgt tccggtaaaa gatactgaag tggttgaacg
     3001 actggagcac accctgcgtg agtttcacga gaagctgcgt gaactgctga cgacgctgaa
     3061 tctgaagctg gaaccggcgg atgattttcg tgatgaaccg gtgaagttaa cggcgtgagt
     3121 gaaatgtgcc ggatgcatca catccggcaa tattcattaa aactgatacg tcatcccaac
     3181 cgcgacagta tcatcattat taatattaag cttattatcg ctatccagtt ggttgatttt
     3241 ataatcaaca aacgctgaca tatttttgtt gaaataatac gtagcaccga cgtcgatata
     3301 attgaccaga tcttcatcac cgatgccctc aatatctttc cctttcgata agacataacc
     3361 cagcgatgga cgcagaccaa agtcaaactg gtattgagcg accgcttcaa agttctgtgt
     3421 cttattggca aagccgccag ttattggcgt cattttgcgt gtttcagaat agaaagttgc
     3481 cagataaata ttattggcat cgtatttcag acctgttgcc catgcttctg cacgcttgcc
     3541 tgtgccacgg ctttgcaggt tctgctcgtt ggtgcgatct gagttggtat aggccccact
     3601 aatggcgaaa tcgctgccgc caaagtcata tgtcaatgac gtgccgaagc catcgccgtt
     3661 ttgcttttta acgtcgcggt tttcgttttt cccttgatat tgcagggtta agttcaggcc
     3721 atcgataacg ccgaagaagt cggtgttccg atacgtcgcc agaccgctgg cgcgtttggt
     3781 cataaagttg tcggtctgcg ccgaggaatc gccaccaaat tccgggaaca tatcggtcca
     3841 ggcttccacg tcatacaacg cgcccaggtt acgaccataa tcgaaagaac ccaaatcttt
     3901 atatttcaac ccggcaaaag cgagacgcgt tttttgctgt gcagtatcac tctccgcttt
     3961 atttccggca aactccgctt cccaccgacc ataaccagtc agttgatcgt taatttgagt
     4021 ttcaccttta aaaccaaaac ggatataact ctggtcgcca tctttactgg cgttatcact
     4081 catataatgc atggctttaa ctttgccata gacatccagt ttattaccgt ctttattata
     4141 tatttctgca gcctgtacag atgcagatgc cacaatgccc atcaccacta atgccagagt
     4201 gctctttttc attttcattc ctgattttaa ttaacgcgcg aatattcagc gggagagccc
     4261 cgttgaaaac aggaaagttt ttaacctgag attgttaaag atatattaca gattaatgat
     4321 attctgaaaa tgtggtaatt tattaaatct gtaataaaag cgtaaacaac tgccgctacg
     4381 cttgctgatc ccgcgcaaca aaacgccatg ctttgctcgc agatggttgg caaccgacga
     4441 cagtcctgct aaaacgttcg tttgatatca tttttcctaa aattgaatgg cagagaatca
     4501 tgagtgacag ccagacgctg gtggtaaaac tcggcaccag tgtgctaaca ggcggatcgc
     4561 gccgtctgaa ccgtgcccat atcgttgaac ttgttcgcca gtgcgcgcag ttacatgccg
     4621 ccgggcatcg gattgttatt gtgacgtcgg gcgcgatcgc cgccggacgt gagcacctgg
     4681 gttacccgga actgccagcg actatcgcct cgaaacaact gctggcggcg gtagggcaga
     4741 gtcgactgat tcaactgtgg gaacagctgt tttcgattta tggcattcac gtcgggcaaa
     4801 tgctgctgac ccgtgctgat atggaagacc gtgaacgctt cctgaacgcc cgcgacaccc
     4861 tgcgtgcgtt gctcgataac aatatcgttc cggtaatcaa tgagaacgat gctgtcgcta
     4921 cggcagagat taaggtcggc gataacgata acctttctgc gctggcggcg attctggcgg
     4981 gtgccgataa actgttgctg ctgaccgatc aaaaaggttt gtataccgct gatccgcgca
     5041 gcaatccgca ggcagaactg attaaagatg tttacggcat tgatgacgca ctgcgcgcga
     5101 ttgccggtga cagcgtttca ggcctcggaa ctggcggcat gagtaccaaa ttgcaggccg
     5161 ctgacgtggc ttgccgtgcg ggtatcgaca ccattattgc cgcgggcagc aagccgggcg
     5221 ttattggtga tgtgatggaa ggcatttccg tcggtacgct gttccatgcc caggcgactc
     5281 cgcttgaaaa ccgtaaacgc tggattttcg gtgcgccgcc ggcgggtgaa atcacggtag
     5341 atgaaggggc aactgccgcc attctggaac gcggcagctc cctgttgccg aaaggcatta
     5401 aaagcgtgac tggcaatttc tcgcgtggtg aagtcatccg catttgcaac ctcgaaggtc
     5461 gcgatatcgc ccacggtgtc agtcgttaca acagcgatgc attacgccgt attgccggac
     5521 accactcgca agaaattgat gcaatactgg gatatgaata cggcccggtt gccgttcacc
     5581 gtgatgacat gattacccgt taaggagctg gccgatgctg gaacaaatgg gcattgccgc
     5641 gaagcaagcc tcgtataaat tagcgcaact ctccagccgc gaaaaaaatc gcgtgctgga
     5701 aaaaatcgcc gatgaactgg aagcacaaag cgaaatcatc ctcaacgcta acgcccagga
     5761 tgttgctgac gcgcgagcca atggccttag cgaagcgatg cttgaccgtc tggcactgac
     5821 gcccgcacgg ctgaaaggca ttgccgacga tgtacgtcag gtgtgcaacc tcgccgatcc
     5881 ggtggggcag gtaatcgatg gcggcgtact ggacagcggc ctgcgtcttg agcgtcgtcg
     5941 cgtaccgctg ggggttattg gcgtgattta tgaagcgcgc ccgaacgtga cggttgatgt
     6001 cgcttcgctg tgcctgaaaa ccggtaatgc ggtgatcctg cgtggtggta aagaaacctg
     6061 tcgcactaac gcggcaacgg tggcggtgat tcaggacgcc ctgaaatcct gtggcttacc
     6121 ggcgggtgcc gtgcaggcga ttgataatcc tgaccgtgcg ctggtcagtg aaatgctgcg
     6181 tatggataaa tacatcgaca tgctgatccc gcgtggtggc gctggtttgc ataaactgtg
     6241 ccgtgaacag tcgacaatcc cggtgatcac aggtggtata ggcgtatgcc atatttacgt
     6301 tgatgaaagt gcagagatcg ctgaagcatt aaaagtgatc gtcaatgcga aaactcagcg
     6361 tccgagcaca tgtaatacgg ttgaaacgtt gctggtgaat aaaaacatcg ccgatagctt
     6421 cctgcccgca ttaagcaaac aaatggcgga aagcggcgtg acattacacg cagatgcagc
     6481 tgcgctgaca cagttgcagg caggccctgc gaaggtggtg gcggttaaag ccgaagagta
     6541 tgacgatgag tttctgtcat tagatttgaa cgtcaaaatc gtcagcgatc ttgacgatgc
     6601 catcgcccat attcgtgaac acggcacaca acactccgat gcgatcctga cccgcgatat
     6661 gcgcaacgcc cagcgttttg ttaacgaagt ggattcgtcc gctgtttacg ttaacgcctc
     6721 tacgcgtttt accgacggcg gccagtttgg tctgggtgcg gaagtggcgg taagcacaca
     6781 aaaactccac gcgcgtggcc caatggggct ggaagcactg accacttaca agtggatcgg
     6841 cattggtgat tacaccattc gtgcgtaaat aaaaccgggt gatgcaaaag tagccatttg
     6901 attcacaagg ccattgacgc atcgcccggt tagttttaac cttgtccacc gtgattcacg
     6961 ttcgtgaaca tgtcctttca gggccgatat agctcagttg gtagagcagc gcattcgtaa
     7021 tgcgaaggtc gtaggttcga ctcctattat cggcaccatt aaaatcaata agttacacat
     7081 cattagtacc ttccttattt tttgactggg acaaatttgg gaccgatggg ttcaggatcg
     7141 agtctatttg ccgtgcgtgt tcggtaaggt gattaggtgc aaggtgagca tatcgacgaa
     7201 ccatttcgat agactcccag cctcccattt cctgtaacac tgacaacggg actccggctt
     7261 gaaccagcca acttgcccag gtgtgtctca agtcgtgaaa tctgaaatca tcaataccag
     7321 cccgtctcag cgccgctttc caggctgtgt ttgcgtcata ccgcatcttc cttactgttg
     7381 gcgctttcgt tccgtctggt ttggtacagc tttccttgta cacaaatacc caacggtgat
     7441 gattcccgat ttgttttttc aaaacgcgac atgcagtatc attcagcgca acgccgattg
     7501 cgcggtttga tttactctct tccgggttta tccatgccac ccggcgctgc atatctattt
     7561 gttgccattc aaggttgatg atgttcgagc gtcttaagcc tgttgccagt gcaaattcaa
     7621 caacagactt taatggctcc ggacattcat caatcagcct ttgtgcttca tggggctcca
     7681 gccagcggat ccgtttattc tttggttgag gcactttaat aattggtgcc ttatccagca
     7741 ttttccattc acgctctgcg gctcttagta gtaatgccag tcagttaagc aactgactgg
     7801 ctctttttc
//
LOCUS       DBKEKI010000418         4023 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.guided.235, whole genome shotgun sequence.
ACCESSION   DBKEKI010000418 DBKEKI010000000
VERSION     DBKEKI010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.guided.235"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            53..1186
                     /locus_tag="ACUQPX_004604"
     CDS             53..1186
                     /locus_tag="ACUQPX_004604"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HHV9058989.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1275..1808
                     /gene="blc"
                     /locus_tag="ACUQPX_004605"
     CDS             1275..1808
                     /gene="blc"
                     /locus_tag="ACUQPX_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HHV9058990.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1805..2122)
                     /gene="sugE"
                     /locus_tag="ACUQPX_004606"
     CDS             complement(1805..2122)
                     /gene="sugE"
                     /locus_tag="ACUQPX_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HHV9058991.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2298..2444)
                     /gene="ecnB"
                     /locus_tag="ACUQPX_004607"
     CDS             complement(2298..2444)
                     /gene="ecnB"
                     /locus_tag="ACUQPX_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HHV9058992.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2555..2680)
                     /gene="ecnA"
                     /locus_tag="ACUQPX_004608"
     CDS             complement(2555..2680)
                     /gene="ecnA"
                     /locus_tag="ACUQPX_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HHV9058993.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(2732..3298)
                     /gene="efp"
                     /locus_tag="ACUQPX_004609"
     CDS             complement(2732..3298)
                     /gene="efp"
                     /locus_tag="ACUQPX_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HHV9058994.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3340..>4023
                     /locus_tag="ACUQPX_004610"
     CDS             3340..>4023
                     /locus_tag="ACUQPX_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HHV9058995.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINH"
BASE COUNT         1024 a         1045 c          997 g          957 t
ORIGIN      
        1 atgtaaatcc ggcccgccta tggcgggccg ttttgtatgg aaaccagacc ttatgttcaa
       61 aacgacgctc tgcgccttat taattaccgc ctcttgctcc acatttgctg cccctcaaca
      121 aatcaacgat attgtgcatc gcacaattac cccgcttata gagcaacaaa agatccccgg
      181 tatggcggtg gcggtaattt atcagggtaa accttattac tttacctggg gctatgcgga
      241 catcgccaaa aagcagcccg tcacacagca aacgttgttt gagttaggtt cggtcagcaa
      301 aacatttacg ggcgtgcttg gtggcgacgc tattgctcga ggggaaatca agttaagcga
      361 tcccgcaaca aaatactggc ctgaacttac cgctaaacag tggaatggga tcacactatt
      421 acatcttgcg acctacaccg ctggcggcct gccattgcag gtgccggatg aagtgaaatc
      481 ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct gcatgggctc caggaacaca
      541 acgtctgtat gccaactcca gtatcggttt gttcggcgca ctggctgtga agccgtctgg
      601 tttgagtttt gagcaggcga tgcaaactcg tgtcttccag ccactcaaac tcaaccatac
      661 gtggattaat gtacctcccc cagaagaaaa gaattacgcc tggggatatc gcgaaggtaa
      721 ggcagtgcat gtttcgccag gggcgttaga tgctgaagct tatggtgtga agtcgaccat
      781 tgaagatatg gcccgctggg tgcaaagcaa tttaaccccc cttgatatca atgagaaaac
      841 gcttcaacaa gggatacaac tggcacaatc tcgctactgg caaaccggcg atatgtatca
      901 gggcctgggc tgggaaatgc tggactggcc ggtcaatcct gacagcatca ttaacggcag
      961 tgacaataaa attgcactgg cagcacgccc ggtaaaagcg attaagcccc caactcctgc
     1021 agtacgcgca tcatgggtac ataaaacggg ggcgaccggc ggatttggta gctatgtcgc
     1081 gtttattcca gaaaaagagc tgggtatcgt gatgctggct aacaaaaact atcccaatcc
     1141 agcgagagtc gccgccgcct gtcagattct caacgctcta cagtaaaatt tcatcgggtc
     1201 cgaattttcg gaccttttct gcgcttttcc ttgctgtcat ctacacttag aaaaaaacca
     1261 gtaaagaaac atttatgcgc ctgctccctc tcgttgccgc agcgacagct gcatttctgg
     1321 tcgttgcctg tagttctcct acgccgccgc gtggcgtgac cgtagtgaat aatttcgacg
     1381 ccaaacgtta tctcggtacc tggtatgaga ttgcccgttt tgatcaccgt tttgaacgtg
     1441 gactggaaaa agtcaccgca acatacagcc tgcgtgatga cggcggcctg aatgtcatta
     1501 ataaaggcta taaccctgac agaggaatgt ggcagcagag tgaagggaaa gcgtacttta
     1561 ccggcgcacc aactcgcgct gcgctgaaag tgtcattctt tggtcctttc tatggcggtt
     1621 ataacgttat tgcactcgat cgggaatacc gccatgcgct ggtttgcggc ccggaccgcg
     1681 actacctgtg gatactctcc cgcacgccaa ccatttctga cgaagtgaaa caggagatgc
     1741 tggcagtcgc gacccgggaa gggtttgatg tcagtaagtt tatttgggta cagcagcccg
     1801 gtagttagtg agtgctgagt ttcagaccaa taatccccaa tacgattaac gccagactag
     1861 ccagacgcat cgggttagcg gactcaccga gcagcacaat gccggtgatg gccgcgccga
     1921 ctgcgccaat acccgtccac acggcataag ccgtccctac cggtaacgat ttcatcgccc
     1981 aggcaagtag cgccatactg actatcatcg ccgtcacggt aataacactc ggcgtcaaac
     2041 ggctaaagcc gtgggtatat ttcaggccaa cggcccatac cacttccagc agaccagcaa
     2101 taactaagat aatccaggac atatcaggct ccggaaattt ggggccgtcc ccggtgaaaa
     2161 gaggcgtttg caggtcgtcc tgcaaagctg atggttgaac ggatattttg ccaggtggca
     2221 ggggaatttc aactattacc tttatgacga tcgcagaaaa aacaaacggc acgacactgc
     2281 tgtcgtaccg ttattgctta ttgctgcgct ttcgttgctg cgccagaaat cgcgttaccg
     2341 ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa tactgttgaa
     2401 agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc ttccttttag
     2461 gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc caaaaatgat
     2521 ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcga gagatggagt
     2581 tgccgagatg tttgatgtct tcgccgaaac cgcaagcggt gttacagccc gtgagcagcg
     2641 tgctggcaag caaaacaaga acgataaggc gtttcatcat ctctgacctg aataagtgat
     2701 ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg tattcaccag
     2761 agcgggtatc cactttgatg acttcgccga tttgtacgaa cagcggaact ttaaccacag
     2821 cgccagtaga cagggtagcc ggtttgccgc cagtacctgc ggtatcacct ttcaggcccg
     2881 gatcggtatc aacgatttcc agttcaacga agttcggcgg agtaacggag atcggctgac
     2941 cattccacag agttacgata cactctgcct gatccagcag ccatttagcg ttgtcgccaa
     3001 ttgcttttgc atcagcagac agctgctcga aagtttcgtt gttcatgaag tgccagaact
     3061 caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcgcct tcagcggaat
     3121 cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc agtttaacgc
     3181 gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc gcgtaaggtt
     3241 cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac gttgccataa
     3301 ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat tgtaacccta
     3361 aataccccat ccagagaaga ttggttaacg caacttgccg atgttgtgac cgatcctgat
     3421 gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac tgttggccgg acgcagcgcc
     3481 aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg atcgcatgga gaaaggcaat
     3541 ccggacgatc ctcttttgcg tcaggtactt acctcgcaag atgagtttgt cgtcgcgtcc
     3601 ggattctcca ccgacccact ggaagaacag cacagcgtag tgcctggttt gttgcataaa
     3661 taccacaacc gggcgctttt gctggtcaaa ggcggctgcg cggtaaattg ccgctattgc
     3721 ttccgtcgcc acttccccta tgccgaaaat cagggcaaca agcgtaactg gcagactgca
     3781 cttgagtatg ttgctgcgca tccggaactg gacgagatga ttttctccgg cggcgatccg
     3841 ctgatggcga aagatcacga gctggactgg ttgctcacac aactggaagc catcccgcat
     3901 atcaaacgtc tgcggattca cagccgtctg ccgattgtga tcccggcacg tatcaccgag
     3961 gcgctggttg aacgctttgc ccgttctacg ctgcaaatct tgctggtgaa tcacatcaat
     4021 cat
//
LOCUS       DBKEKI010000419         2365 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.guided.274, whole genome shotgun sequence.
ACCESSION   DBKEKI010000419 DBKEKI010000000
VERSION     DBKEKI010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.guided.274"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            267..1127
                     /locus_tag="ACUQPX_004611"
     CDS             267..1127
                     /locus_tag="ACUQPX_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HHV9058996.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            1284..1643
                     /locus_tag="ACUQPX_004612"
     CDS             1284..1643
                     /locus_tag="ACUQPX_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization domain protein"
                     /protein_id="HHV9058997.1"
                     /translation="MSLAEKVFGAVETVEGVAQRLNAKIMGSVQPMAEKAAVSAQNER
                     RAKELRETLAQQQKRLQALQEPFKGLSKDQVAGLIRQAVKLRQENEQEKQERAQQIKE
                     RFKAKRERERSDRSRGR"
     gene            complement(1878..2099)
                     /locus_tag="ACUQPX_004613"
     CDS             complement(1878..2099)
                     /locus_tag="ACUQPX_004613"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058998.1"
                     /translation="MAMSMYYIKTGLEYWKPKGYGYTKSREEAGVFTLKDMEELNLDG
                     CTLERAEVAFLDEDGPSPDLPHRVTLPRD"
     gene            complement(2090..2293)
                     /locus_tag="ACUQPX_004614"
     CDS             complement(2090..2293)
                     /locus_tag="ACUQPX_004614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9058999.1"
                     /translation="MTDPLKALFGKPDYSHIVRDTTATISITAAEMAAVLEAYDRGID
                     TLDGTTRTALDSVISKLKDEVWP"
BASE COUNT          614 a          573 c          598 g          580 t
ORIGIN      
        1 atgataagtt tatcaccacc gactatttgc aacagtgccc gctccacggt ttataaaatt
       61 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      121 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      181 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tggtaaatgc
      241 ttcaataata ttgaaaaagg aagagtatga gtattcaaca ttttcgtgtc gcccttattc
      301 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      361 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      421 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      481 ttctgctatg tggtgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
      541 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
      601 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
      661 ctgccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
      721 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
      781 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat
      841 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
      901 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
      961 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1021 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1081 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1141 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1201 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1261 gagcgtcaga ccccgtcaag ggggtgtcac tggccgagaa ggtgttcgga gccgtggaga
     1321 cggtcgaagg ggtggcgcaa aggttgaacg ccaaaataat ggggtctgtg cagccgatgg
     1381 ccgagaaagc cgctgtaagc gcccagaacg agcgaagagc caaggagcta agggaaaccc
     1441 tagcccagca gcaaaaacgc ttacaggccc ttcaggagcc gtttaagggg ctatccaagg
     1501 atcaggtagc gggactgatc cggcaggcgg tgaaattgcg gcaggagaac gagcaggaga
     1561 agcaggagcg cgcccagcag atcaaagagc gattcaaggc caagcgggaa cgggagcgca
     1621 gcgaccgcag caggggccgt taaccctttt caaccaactt taaccaacct tgaacaacct
     1681 taaaccattg tttaaccaac attaaaccac tttcaaccaa ccctaaacca ctttcaacca
     1741 accctaagca accggggtgt aagggctggc agtaatcgcg cggatttaca ggcacgtcgg
     1801 atatgtttac aaaaactccg tttttgacac atatcccgtg ccaaagggtg cccctttgaa
     1861 accctccagc gccccgctca atcgcggggc agggtgacgc ggtggggtag gtcgggactg
     1921 gggccgtcct cgtccaggaa cgccacttcg gcgcgttcca gggtgcagcc atcgaggttc
     1981 aattcttcca tgtccttgag ggtgaaaacc cccgcctcct cgcggctctt ggtgtacccg
     2041 tagcccttgg gtttccagta ctccagacct gtcttgatgt aatacatgct catggccata
     2101 cctcgtcctt gagtttcgat ataacggaat ccagcgccgt tctggtggtg ccgtccagcg
     2161 tgtctatacc cctgtcgtaa gcctccaaaa cagcggccat ttcagcggct gtgatgctga
     2221 tagtggccgt ggtgtccctt acgatatggg agtaatcagg cttgccgaaa agggctttga
     2281 gtggatctgt catggctgat ctccaaacat atcaagcgtg tctgtatccc actctattgt
     2341 aaacaagaca tttttatctt ttata
//
LOCUS       DBKEKI010000420         2087 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.guided.282, whole genome shotgun sequence.
ACCESSION   DBKEKI010000420 DBKEKI010000000
VERSION     DBKEKI010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.guided.282"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            37..1242
                     /gene="tet(B)"
                     /locus_tag="ACUQPX_004615"
     CDS             37..1242
                     /gene="tet(B)"
                     /locus_tag="ACUQPX_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HHV9059000.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1355..1948)
                     /gene="tetC"
                     /locus_tag="ACUQPX_004616"
     CDS             complement(1355..1948)
                     /gene="tetC"
                     /locus_tag="ACUQPX_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HHV9059001.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          551 a          371 c          436 g          729 t
ORIGIN      
        1 ttttaccact ccctatcagt gatagagaaa agtgaaatga atagttcgac aaagatcgca
       61 ttggtaatta cgttactcga tgccatgggg attggcctta tcatgccagt cttgccaacg
      121 ttattacgtg aatttattgc ttcggaagat atcgctaacc actttggcgt attgcttgca
      181 ctttatgcgt taatgcaggt tatctttgct ccttggcttg gaaaaatgtc tgaccgattt
      241 ggtcggcgcc cagtgctgtt gttgtcatta ataggcgcat cgctggatta cttattgctg
      301 gctttttcaa gtgcgctttg gatgctgtat ttaggccgtt tgctttcagg gatcacagga
      361 gctactgggg ctgtcgcggc atcggtcatt gccgatacca cctcagcttc tcaacgcgtg
      421 aagtggttcg gttggttagg ggcaagtttt gggcttggtt taatagcggg gcctattatt
      481 ggtggttttg caggagagat ttcaccgcat agtccctttt ttatcgctgc gttgctaaat
      541 attgtcgctt tccttgtggt tatgttttgg ttccgtgaaa ccaaaaatac acgtgataat
      601 acagataccg aagtaggggt tgagacgcaa tcgaattcgg tatacatcac tttatttaaa
      661 acgatgccca ttttgttgat tatttatttt tcagcgcaat tgataggcca aattcccgca
      721 acggtgtggg tgctatttac cgaaaatcgt tttggatgga atagcatgat ggttggcttt
      781 tcattagcgg gtcttggtct tttacactca gtattccaag cctttgtggc aggaagaata
      841 gccactaaat ggggcgaaaa aacggcagta ctgctcggat ttattgcaga tagtagtgca
      901 tttgcctttt tagcgtttat atctgaaggt tggttagttt tccctgtttt aattttattg
      961 gctggtggtg ggatcgcttt acctgcatta cagggagtga tgtctatcca aacaaagagt
     1021 catcagcaag gtgctttaca gggattattg gtgagcctta ccaatgcaac cggtgttatt
     1081 ggcccattac tgtttgctgt tatttataat cattcactac caatttggga tggctggatt
     1141 tggattattg gtttagcgtt ttactgtatt attatcctgc tatcgatgac cttcatgtta
     1201 acccctcaag ctcaggggag taaacaggag acaagtgctt agttatttcg tcaccaaatg
     1261 atgttattcc gcgaaatata atgaccctct tgataaccca agagggcatt ttttacgata
     1321 aagaagattt agcttcaaat aaaacctatc tattttattt atctttcaag ctcaataaaa
     1381 agccgcggta aatagcaata aattggcctt ttttatcggc aagctctttt aggtttttcg
     1441 catgtattgc gatatgcata aaccagccat tgagtaagtt tttaagcaca tcatcatcat
     1501 aagctttaag ttggttctct tggatcaatt tgctgacaat ggcgtttacc ttaccagtaa
     1561 tgtattcaag gctaattttt tcaagttcat tccaaccaat gataggcatc acttcttgga
     1621 tagggataag gtttttatta ttatcaataa tataatcaag ataatgttca aatatacttt
     1681 ctaaggcaga ccaaccattt gttaaatcag tttttgttgt gatgtaggca tcaatcataa
     1741 ttaattgctg cttataacag gcactgagta attgtttttt atttttaaag tgatgataaa
     1801 aggcaccttt ggtcaccaac gcttttcccg agatctcatc tattgaaaca gcttgatagc
     1861 ctttttcaac aaacaatatt cgtgctgagt taaccagtga ttgataggta ctcttaaaat
     1921 tttcttgttg atgattttta ttttccatga tagatttaaa ataacatacc gtcagtatgt
     1981 ttatggtatc atgatgatgt ggtcgtgaca atcttaagaa catttaggtt attttatgta
     2041 tattgaacag cattctcgct atcaaaataa agctaataac atccaat
//
LOCUS       DBKEKI010000421         1853 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.guided.292, whole genome shotgun sequence.
ACCESSION   DBKEKI010000421 DBKEKI010000000
VERSION     DBKEKI010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.guided.292"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            69..872
                     /locus_tag="ACUQPX_004617"
                     /note="aph(3'')-Ib"
     CDS             69..872
                     /locus_tag="ACUQPX_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HHV9059002.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            872..1708
                     /locus_tag="ACUQPX_004618"
     CDS             872..1708
                     /locus_tag="ACUQPX_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HHV9059003.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSVAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          393 a          500 c          524 g          436 t
ORIGIN      
        1 ttcgttaact gcacattcgg gatatttctc tatattcgcg cttcatcaga aaactgaagg
       61 aacctccatt gaatcgaact aatatttttt ttggtgaatc gcattctgac tggttgcctg
      121 tcagaggcgg agaatctggt gattttgttt ttcgacgtgg tgacgggcat gccttcgcga
      181 aaatcgcacc tgcttcccgc cgcggtgagc tcgctggaga gcgtgaccgc ctcatttggc
      241 tcaaaggtcg aggtgtggct tgccccgagg tcatcaactg gcaggaggaa caggagggtg
      301 catgcttggt gataacggca attccgggag taccggcggc tgatctgtct ggagcggatt
      361 tgctcaaagc gtggccgtca atggggcagc aacttggcgc tgttcacagc ctatcggttg
      421 atcaatgtcc gtttgagcgc aggctgtcgc gaatgttcgg acgcgccgtt gatgtggtgt
      481 cccgcaatgc cgtcaatccc gacttcttac cggacgagga caagagtacg ccgcagctcg
      541 atcttttggc tcgtgtcgaa cgagagctac cggtgcggct cgaccaagag cgcaccgata
      601 tggttgtttg ccatggtgat ccctgcatgc cgaacttcat ggtggaccct aaaactcttc
      661 aatgcacggg tctgatcgac cttgggcggc tcggaacagc agatcgctat gccgatttgg
      721 cactcatgat tgctaacgcc gaagagaact gggcagcgcc agatgaagca gagcgcgcct
      781 tcgctgtcct attcaatgta ttggggatcg aagcccccga ccgcgaacgc cttgccttct
      841 atctgcgatt ggaccctctg acttggggtt gatgttcatg ccgcctgttt ttcctgctca
      901 ttggcacgtt tcgcaacctg ttctcattgc ggacaccttt tccagcctcg tttggaaagt
      961 ttcattgcca gacgggactc ctgcaatcgt caagggattg aaacctataa aagacattgc
     1021 tgatgaactg cgcggggccg actatctggt atggcgcaat gggaggggag cagtccggtt
     1081 gctcggtcgt gagaacaatc tgatgttgct cgaatatgcc ggggagcgaa tgctctctca
     1141 catcgttgcc gagcacggcg actaccaggc gaccgaaatt gcagcggaac taatggcgaa
     1201 gctgtatgcc gcatctgagg aacccctgcc ttctgccctt ctcccgatcc gggatcgctt
     1261 tgcagctttg tttcagcggg cgcgcgatga tcaaaacgca ggttgtcaaa ctgactacgt
     1321 ccacgcggcg attatagccg atcaaatgat gagcaatgcc tcggaactgc gtgggctaca
     1381 tggcgatctg catcatgaaa acatcatgtt ctccagtcgc ggctggctgg tgatagatcc
     1441 cgtcggtctg gtcggtgaag tgggctttgg cgccgccaat atgttctacg atccggctga
     1501 cagagacgac ctttgtctcg atcctagacg cattgcacag atggcggacg cattctctcg
     1561 tgcgctggac gtcgatccgc gtcgcctgct cgaccaggcg tacgcttatg ggtgcctttc
     1621 cgtagcttgg aacgcggatg gagaagagga gcaacgcgat ctagctatcg cggccgcgat
     1681 caagcaggtg cgacagacgt catactagat atcaagcgac ttctcctatc ccctgggaac
     1741 acatcaatct caccggagaa tatcgctggc caaagcctta gcgtaggatt ccgccccttc
     1801 ccgcaaacga ccccaaacag gaaacgcagc tgaaacggga agctcaacac cca
//
LOCUS       DBKEKI010000422          906 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.guided.351, whole genome shotgun sequence.
ACCESSION   DBKEKI010000422 DBKEKI010000000
VERSION     DBKEKI010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.guided.351"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            83..592
                     /gene="dfrA8"
                     /locus_tag="ACUQPX_004619"
     CDS             83..592
                     /gene="dfrA8"
                     /locus_tag="ACUQPX_004619"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA8"
                     /protein_id="HHV9059004.1"
                     /translation="MIELHAILAATANGCIGKDNALPWPPLKGDLARFKKLTMGKVVI
                     MGRKTYESLPVKLEGRTCIVMTRQALELPGVRDANGAIFVNNVSDAMRFAQEESVGDV
                     AYVIGGAEIFKRLALMITQIELTFVKRLYEGDTYVDLAEMVKDYEQNGMEEHDLHTYF
                     TYRKKELTE"
     gene            589..>906
                     /locus_tag="ACUQPX_004620"
     CDS             589..>906
                     /locus_tag="ACUQPX_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HHV9059005.1"
                     /translation="MTFVAISDTHLHNWSQFAIPTESGINSRLLQILKAIEEAACAAD
                     YHAPAGVVPTVYHGGDLFHVRGSLTPSVLNAVLDFFKTIHRDYGVRFRMIAGNHDLET
                     KDSC"
BASE COUNT          228 a          242 c          226 g          210 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccc gacgcaataa tcaccaattc
       61 ctcttttgat aggtaaacaa caatgatcga gcttcatgcc attttagctg ccaccgccaa
      121 tggttgcatt gggaaggaca acgcacttcc ctggccacca ctaaaaggcg atctggccag
      181 attcaaaaaa ttgaccatgg ggaaggtggt cattatgggg cgcaagacct atgagagctt
      241 gcccgtcaaa ttagaaggtc gcacctgcat cgttatgacg cgccaagcgc tggagcttcc
      301 gggtgttcgt gacgctaacg gcgctatctt cgtgaacaac gtcagcgacg ccatgcggtt
      361 cgctcaagaa gagagcgtgg gcgatgtggc ctacgtcatt ggtggcgctg agatattcaa
      421 gcgacttgcc ttgatgatca cgcagattga attgaccttt gttaagcgac tgtacgaagg
      481 cgacacctac gttgatctgg ccgaaatggt caaagactac gagcagaatg gcatggaaga
      541 acatgacctt cacacttact tcacttaccg taaaaaggag cttacagaat gaccttcgtt
      601 gccatttcag atacgcacct gcacaactgg tcgcagttcg cgataccaac agagtcgggc
      661 attaactcca ggctgttgca gattcttaaa gccattgaag aagcggcttg cgccgccgat
      721 taccacgccc cagcgggcgt ggttccaacc gtctatcacg gcggcgatct cttccatgtg
      781 cgaggttctt tgaccccttc tgtgctaaac gcggtgctcg acttttttaa gaccatccac
      841 cgtgactacg gcgttcgctt tcgcatgatc gccggcaatc acgatctgga aaccaaagac
      901 tcatgt
//
LOCUS       DBKEKI010000423          869 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003641-006961
            SAMN51231842-rid27608723.guided.354, whole genome shotgun sequence.
ACCESSION   DBKEKI010000423 DBKEKI010000000
VERSION     DBKEKI010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51231842
            Sequence Read Archive: SRR35312013
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918969.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:44:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,554
            Genes (coding)                    :: 4,185
            CDSs (with protein)               :: 4,185
            Genes (RNA)                       :: 67
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 369
            CDSs (without protein)            :: 369
            Pseudo Genes (ambiguous residues) :: 0 of 369
            Pseudo Genes (frameshifted)       :: 174 of 369
            Pseudo Genes (incomplete)         :: 149 of 369
            Pseudo Genes (internal stop)      :: 103 of 369
            Pseudo Genes (multiple problems)  :: 53 of 369
            Pseudo Genes (short protein)      :: 1 of 369
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51231842-rid27608723.guided.354"
                     /strain="EF0003641-006961"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-06-07"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            22..837
                     /gene="sul2"
                     /locus_tag="ACUQPX_004621"
     CDS             22..837
                     /gene="sul2"
                     /locus_tag="ACUQPX_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HHV9059006.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          155 a          262 c          255 g          197 t
ORIGIN      
        1 attattatta tagaagcccc catgaataaa tcgctcatca ttttcggcat cgtcaacata
       61 acctcggaca gtttctccga tggaggccgg tatctggcgc cagacgcagc cattgcgcag
      121 gcgcgtaagc tgatggccga gggggcagat gtgatcgacc tcggtccggc atccagcaat
      181 cccgacgccg cgcctgtttc gtccgacaca gaaatcgcgc gtatcgcgcc ggtgctggac
      241 gcgctcaagg cagatggcat tcccgtctcg ctcgacagtt atcaacccgc gacgcaagcc
      301 tatgccttgt cgcgtggtgt ggcctatctc aatgatattc gcggttttcc agacgctgcg
      361 ttctatccgc aattggcgaa atcatctgcc aaactcgtcg ttatgcattc ggtgcaagac
      421 gggcaggcag atcggcgcga ggcacccgct ggcgacatca tggatcacat tgcggcgttc
      481 tttgacgcgc gcatcgcggc gctgacgggt gccggtatca aacgcaaccg ccttgtcctt
      541 gatcccggca tggggttttt tctgggggct gctcccgaaa cctcgctctc ggtgctggcg
      601 cggttcgatg aattgcggct gcgcttcgat ttgccggtgc ttctgtctgt ttcgcgcaaa
      661 tcctttctgc gcgcgctcac aggccgtggt ccgggggatg tcggggccgc gacactcgct
      721 gcagagcttg ccgccgccgc aggtggagct gacttcatcc gcacacacga gccgcgcccc
      781 ttgcgcgacg ggctggcggt attggcggcg ctgaaagaaa ccgcaagaat tcgttaactg
      841 cacattcggg atatttctct atattcgcg
//
